LOCUS       DAPHDW010000001        95548 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.001, whole genome shotgun sequence.
ACCESSION   DAPHDW010000001 DAPHDW010000000
VERSION     DAPHDW010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 95548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.001"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            50..568
                     /locus_tag="RH057_000001"
     CDS             50..568
                     /locus_tag="RH057_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV3126426.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQVGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            584..1123
                     /locus_tag="RH057_000002"
     CDS             584..1123
                     /locus_tag="RH057_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HDV3126427.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEASADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(1218..2795)
                     /gene="guaA"
                     /locus_tag="RH057_000003"
     CDS             complement(1218..2795)
                     /gene="guaA"
                     /locus_tag="RH057_000003"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HDV3126428.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPVFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(2864..4330)
                     /gene="guaB"
                     /locus_tag="RH057_000004"
     CDS             complement(2864..4330)
                     /gene="guaB"
                     /locus_tag="RH057_000004"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HDV3126429.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            4492..5862
                     /gene="xseA"
                     /locus_tag="RH057_000005"
     CDS             4492..5862
                     /gene="xseA"
                     /locus_tag="RH057_000005"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HDV3126430.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAEGLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTAVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     TRQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRTGQQQQRLTQRLNQQNPQPKIHRAQTRIQQLEYRLAETLRVQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKAGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            complement(5859..6074)
                     /locus_tag="RH057_000006"
     CDS             complement(5859..6074)
                     /locus_tag="RH057_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HDV3126431.1"
                     /translation="MELHCPQCQHVLDQDNGHARCPSCGEIIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(6143..7615)
                     /gene="der"
                     /locus_tag="RH057_000007"
     CDS             complement(6143..7615)
                     /gene="der"
                     /locus_tag="RH057_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HDV3126432.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVANKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            complement(7733..8911)
                     /gene="bamB"
                     /locus_tag="RH057_000008"
     CDS             complement(7733..8911)
                     /gene="bamB"
                     /locus_tag="RH057_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HDV3126433.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGLTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWTQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            complement(8922..9542)
                     /gene="yfgM"
                     /locus_tag="RH057_000009"
     CDS             complement(8922..9542)
                     /gene="yfgM"
                     /locus_tag="RH057_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HDV3126434.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGVILGVGALIGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            complement(9560..10834)
                     /gene="hisS"
                     /locus_tag="RH057_000010"
     CDS             complement(9560..10834)
                     /gene="hisS"
                     /locus_tag="RH057_000010"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HDV3126435.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGISEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELPGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            complement(10945..12063)
                     /gene="ispG"
                     /locus_tag="RH057_000011"
                     /gene_synonym="gcpE"
     CDS             complement(10945..12063)
                     /gene="ispG"
                     /locus_tag="RH057_000011"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HDV3126436.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVEK"
     gene            complement(12090..13103)
                     /gene="rodZ"
                     /locus_tag="RH057_000012"
     CDS             complement(12090..13103)
                     /gene="rodZ"
                     /locus_tag="RH057_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HDV3126437.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPAPTATTTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            complement(13388..14542)
                     /gene="trmG/rlmN"
                     /locus_tag="RH057_000013"
     CDS             complement(13388..14542)
                     /gene="trmG/rlmN"
                     /locus_tag="RH057_000013"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HDV3126438.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKPFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPGAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            complement(14692..15123)
                     /gene="ndk"
                     /locus_tag="RH057_000014"
     CDS             complement(14692..15123)
                     /gene="ndk"
                     /locus_tag="RH057_000014"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HDV3126439.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            complement(15272..17584)
                     /gene="pbpC"
                     /locus_tag="RH057_000015"
     CDS             complement(15272..17584)
                     /gene="pbpC"
                     /locus_tag="RH057_000015"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HDV3126440.1"
                     /translation="MPRLLTKRGCWITLAAAPFLLFLAAWGADKLWPLPLHEVNPARV
                     VVAQDGTPLWRFADADGIWRYPVTIEDVSPRYLEALINYEDRWFWKHPGVNPFSVARA
                     AWQDLTSGRVISGGSTLTMQVARLLDPHPKTFGGKIRQLWRALQLEWHLSKREILTLY
                     LNRAPFGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQAPSRLRPDRWPERAEAA
                     RNKVLERMAVQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTL
                     DAGLQRRLEELAQNWKGRLPPRSSLAMIVVDHTDMRVRGWVGSVDLNDDSRFGHVDMV
                     NAIRSPGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPISMSEA
                     LVRSLNLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAA
                     AYTAFARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAKPLPDSALPRVAPL
                     AWKTGTSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLS
                     RSANLPEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLPEQEG
                     INGIRFPIWLDENGKRVAADCPQARQEMINVWPLPLEPWLPASERRAVRLPPASTICP
                     PYGHDAKLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRN
                     VTLHLTDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            complement(17585..22546)
                     /gene="yfhM"
                     /locus_tag="RH057_000016"
     CDS             complement(17585..22546)
                     /gene="yfhM"
                     /locus_tag="RH057_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha2-macroglobulin"
                     /protein_id="HDV3126441.1"
                     /translation="MKKLRVAACMLMLALAGCDNNDNAPTAVKKDAPSEVTKAASSEN
                     ASSAKLSVPERQKLAQQSAGKVLTLLDLSEVQLDGAATLVLTFSIPLDPDQDFSRVIH
                     VVDKKSGKVDGAWELSDNLKELRLRHLEPKRDLIVTIGKEVKALNNATFSKDYEKTIT
                     TRDIQPSVGFASRGSLLPGKVVEGLPVMALNVNNVDVNFFRVKPESLPAFISQWEYRN
                     SLANWQSDKLLQMADLVYTGRFDLNPARNTREKLLLPLGDIKPLQQAGVYLAVMNQAG
                     RYDYSNPATLFTLSDIGVSAHRYHNRLDIFTQSLENGAAQQGIEVSLLNEKGQTLTQA
                     TSDAQGHVQLENDKNAALLLARKDGQTTLLDLKLPALDLAEFNIAGAPGYSKQFFMFG
                     PRDLYRPGETVILNGLLRDADGKALPNQPIKLDVIKPDGQVLRSVVSQPENGLYHFTW
                     PLDSNAATGMWHIRANTGDNQYRMWDFHVEDFMPERMALNLTGEKTPLTPKDEVKFSV
                     VGYYLYGAPANGNTLQGQLFLRPLREAVSALPGFEFGDIAAENLSRTLDEVQLTLDDK
                     GRGEVSTESQWKETHSPLQVIFQGSLLESGGRPVTRRAEQAIWPADALPGIRPQFASK
                     SVYDYRTDSTVKQPIVDEGSNAAFDIVYSDAQGVKKAVSGLQVRLIRERRDYYWNWSE
                     DEGWQSQFDQKDLIENEQTLDLKADETGKVSFPVEWGAYRLEVKAPNEAVSSVRFWAG
                     YSWQDNSDGSGAVRPDRVTLKLDKASYRPGDTIKLHIAAPTAGKGYAMVESSEGPLWW
                     QEIDVRAQGLDLTIPVDKTWNRHDLYLSTLVVRPGDKSRSATPKRAVGVLHLPLGDEN
                     RRLDLALETPAKMRPNQPLTVKIKASTKNGEKPKQVNVLVSAVDSGVLNITDYVTPDP
                     WQAFFGQKRYGADIYDIYGQVIEGQGRLAALRFGGDGDELKRGGKPPVNHVNIVAQQA
                     LPVTLNEQGEGSVTLPIGDFNGELRVMAQAWTADDFGSNESKVIVAAPVIAELNMPRF
                     MASGDTSRLTLDITNLTDKPQKLNVALTASGLLELVSDSPAAVELAPGVRTTLFIPVR
                     ALPGYGDGEIQATISGLALPGETVADQHKQWKIGVRPAFPAQTVNYGTALQPGETWAI
                     PADGLQNFSPVTLEGQLLLSGKPPLNIARYIKELKAYPYGCLEQTASGLFPSLYTNAA
                     QLQALGIKGDSDEKRRASVDIGISRLLQMQRDNGGFALWDKNGDEEYWLTAYVMDFLV
                     RAGEQGYSVPTDAINRGNERLLRYLQDPGMMSIPYADNLKASKFAVQSYAALVLARQQ
                     KAPLGALREIWEHRADAASGLPLLQLGVALKTMGDATRGEEAIVLALKTPRNSDERIW
                     LGDYGSPLRDNALMLSLLEENKLLPDEQYTLLNTLSQQAFGERWLSTQESNALFLAAR
                     TIQDLPGKWQAQTSFSAEPLTGEKTLNSNLNSDQLATLQVRNSGDQPLWLRMDASGYP
                     QSAPLPANNVLQIERHILGTDGKSKSLDSLRSGDLVLVWLQVKASNSVPDALVVDLLP
                     AGLELENQNLANGSASLEQSGGEVQNLLNQMQQASIKHIEFRDDRFVAAVAVDEYQPV
                     TLVYLARAVTPGTYQVPQPMVESMYVPQWRATGAAEDLLIVRP"
     gene            22753..23598
                     /gene="sseA"
                     /locus_tag="RH057_000017"
     CDS             22753..23598
                     /gene="sseA"
                     /locus_tag="RH057_000017"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HDV3126442.1"
                     /translation="MSTTWFVGADWLAEHIDDPEIQIIDARMASPGQEDRNVAQEYLN
                     GHIPGAVFFDIEALSDHTSPLPHMLPRPETFAVAMRELGVNQDKHLIVYDEGNLFSAP
                     RAWWMLRTFGVEKVSILGGGLAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDV
                     LLASHENTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDE
                     LDAIFFGRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLP
                     VEPVK"
     gene            complement(24091..24867)
                     /gene="sseB"
                     /locus_tag="RH057_000018"
     CDS             complement(24091..24867)
                     /gene="sseB"
                     /locus_tag="RH057_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HDV3126443.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKSVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIVPFYERRWGGFLRDFKQNRII"
     gene            complement(25009..26292)
                     /gene="pepB"
                     /locus_tag="RH057_000019"
     CDS             complement(25009..26292)
                     /gene="pepB"
                     /locus_tag="RH057_000019"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HDV3126444.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKAPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAITRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(26470..26670)
                     /gene="iscX"
                     /locus_tag="RH057_000020"
     CDS             complement(26470..26670)
                     /gene="iscX"
                     /locus_tag="RH057_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HDV3126445.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(26682..27017)
                     /gene="fdx"
                     /locus_tag="RH057_000021"
     CDS             complement(26682..27017)
                     /gene="fdx"
                     /locus_tag="RH057_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HDV3126446.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(27019..28869)
                     /gene="hscA"
                     /locus_tag="RH057_000022"
     CDS             complement(27019..28869)
                     /gene="hscA"
                     /locus_tag="RH057_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HDV3126447.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQAGIPDRSDNRVQRELLDAAIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDDAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(28886..29401)
                     /gene="hscB"
                     /locus_tag="RH057_000023"
     CDS             complement(28886..29401)
                     /gene="hscB"
                     /locus_tag="RH057_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HDV3126448.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSQAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(29497..29820)
                     /gene="iscA"
                     /locus_tag="RH057_000024"
     CDS             complement(29497..29820)
                     /gene="iscA"
                     /locus_tag="RH057_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HDV3126449.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(29837..30223)
                     /gene="iscU"
                     /locus_tag="RH057_000025"
     CDS             complement(29837..30223)
                     /gene="iscU"
                     /locus_tag="RH057_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HDV3126450.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(30251..31465)
                     /gene="iscS"
                     /locus_tag="RH057_000026"
     CDS             complement(30251..31465)
                     /gene="iscS"
                     /locus_tag="RH057_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HDV3126451.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(31577..32065)
                     /gene="iscR"
                     /locus_tag="RH057_000027"
     CDS             complement(31577..32065)
                     /gene="iscR"
                     /locus_tag="RH057_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HDV3126452.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRIQDAIDVKL
                     RA"
     gene            complement(32366..33103)
                     /gene="trmJ"
                     /locus_tag="RH057_000028"
     CDS             complement(32366..33103)
                     /gene="trmJ"
                     /locus_tag="RH057_000028"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HDV3126453.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKA"
     gene            33222..34025
                     /gene="suhB"
                     /locus_tag="RH057_000029"
     CDS             33222..34025
                     /gene="suhB"
                     /locus_tag="RH057_000029"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000553460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HDV3126454.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAMLANMRDELSDALKR"
     gene            34170..35024
                     /locus_tag="RH057_000030"
     CDS             34170..35024
                     /locus_tag="RH057_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417029.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3126455.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     YPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQRLVLFGQSIGGA
                     NILDVIGRGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGDVYREQMVDFI
                     LSALNPQN"
     gene            35215..36495
                     /gene="csiE"
                     /locus_tag="RH057_000031"
     CDS             35215..36495
                     /gene="csiE"
                     /locus_tag="RH057_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HDV3126456.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATTATFSELNGVDD
                     DIASLDISATGQEILRYHQLTLTAGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSHFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFETRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQPVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYTHLAQALNRSFF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(36487..37626)
                     /locus_tag="RH057_000032"
     CDS             complement(36487..37626)
                     /locus_tag="RH057_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HDV3126457.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLTLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     ALLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQGSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(37786..38676)
                     /gene="hcaR"
                     /locus_tag="RH057_000033"
     CDS             complement(37786..38676)
                     /gene="hcaR"
                     /locus_tag="RH057_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HDV3126458.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KPALRDFIAIVQERLASVTA"
     gene            38812..40173
                     /locus_tag="RH057_000034"
     CDS             38812..40173
                     /locus_tag="RH057_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HDV3126459.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNTRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLRDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            40170..40688
                     /locus_tag="RH057_000035"
     CDS             40170..40688
                     /locus_tag="RH057_000035"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HDV3126460.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QISETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            40688..41008
                     /locus_tag="RH057_000036"
     CDS             40688..41008
                     /locus_tag="RH057_000036"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HDV3126461.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            41005..41817
                     /gene="hcaB"
                     /locus_tag="RH057_000037"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             41005..41817
                     /gene="hcaB"
                     /locus_tag="RH057_000037"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HDV3126462.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTASK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            41827..43029
                     /locus_tag="RH057_000038"
     CDS             41827..43029
                     /locus_tag="RH057_000038"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HDV3126463.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLRHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVVDGEKV
                     ELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIFAGGDV
                     AITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQFIGDM
                     RGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDAKLLID
                     ENIALKSL"
     gene            43126..43548
                     /locus_tag="RH057_000039"
     CDS             43126..43548
                     /locus_tag="RH057_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV3126464.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(43596..44468)
                     /locus_tag="RH057_000040"
     CDS             complement(43596..44468)
                     /locus_tag="RH057_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV3126465.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQPAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDDHHRPEGGDLLALAPG
                     ESTTSEMSLRVALL"
     gene            complement(44480..45574)
                     /locus_tag="RH057_000041"
     CDS             complement(44480..45574)
                     /locus_tag="RH057_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV3126466.1"
                     /translation="MKINRIVNEGFMKTMLAAYLPGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGVMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYALMASGKCGKVVINFPD"
     gene            complement(45607..46605)
                     /locus_tag="RH057_000042"
     CDS             complement(45607..46605)
                     /locus_tag="RH057_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3126467.1"
                     /translation="MSASSLSLPQGKSVSLKQFVSRHINEIGFLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAVACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMIVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(46630..48141)
                     /locus_tag="RH057_000043"
     CDS             complement(46630..48141)
                     /locus_tag="RH057_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3126468.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASC
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLEEYEQGEIVINGEKITRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISANGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(48164..49147)
                     /locus_tag="RH057_000044"
     CDS             complement(48164..49147)
                     /locus_tag="RH057_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV3126469.1"
                     /translation="MPKKMRTTRNLLLMATLLGSALFARAADKEMTIGAIYLDTQGYY
                     AGVRQGVQDAAKDSSVQVQLIETNAQGDISKESTFVDTLVARNVDAIILSAVSENGSS
                     RTVRRASEAGIPVICYNTCINQKGVDKYVSAYLVGDPLEFGKKLGNAAADYFIANKID
                     QPKIAVINCEAFEVCVQRRKGFEEVLKSRVPGAQIVANQEGTVLDKAISVGEKLIIST
                     PDLNAIMGESGGATLGAVKAVRNQNQAGKIAVFGSDMTTEIAQELENNQVLKAVVDIS
                     GKKMGNAVFAQTLKVINKQADGEKVIQVPIDLYTKTEDGKQWLATHVDGLP"
     gene            complement(49244..52525)
                     /locus_tag="RH057_000045"
     CDS             complement(49244..52525)
                     /locus_tag="RH057_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HDV3126470.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKSWQAVWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPA
                     LVGLLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMT
                     GFTLRPDRAALEIASRVYNGNATPRHFLWWANPAVKGGEGHQSVFPPDVTAVFDHGKR
                     AVSAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLH
                     VANHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEE
                     KRFEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCN
                     ALLDDAVALMPATAIQGVLHGINPERLTIELSDADGNIVLSYQEHQPQELPLPDVAKA
                     PLSAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNR
                     ADFPQAVAYATQALKRAHALNKNPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGN
                     SKAGGYYGLARLAARNGNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARV
                     QREKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTL
                     ATEMLNALDCQRTLSLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESI
                     EECWFARHLLACFYYNKRSYNKAITLWQSCVEMSPEFADGWRGLAIHAWNKQHDYELA
                     ARYLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLN
                     LWHLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDARQPQQASELL
                     HAALHYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHS
                     YYNDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLL
                     SLYGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFI
                     FNYVH"
     gene            52643..53836
                     /locus_tag="RH057_000046"
     CDS             52643..53836
                     /locus_tag="RH057_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HDV3126471.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDAPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVMVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGCLETVASLSALKKQARVWLK
                     SQPVNTQLDPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(53900..55153)
                     /gene="glyA"
                     /locus_tag="RH057_000047"
     CDS             complement(53900..55153)
                     /gene="glyA"
                     /locus_tag="RH057_000047"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HDV3126472.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            55481..56671
                     /gene="hmpA"
                     /locus_tag="RH057_000048"
     CDS             55481..56671
                     /gene="hmpA"
                     /locus_tag="RH057_000048"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HDV3126473.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISAGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGDVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(56716..57054)
                     /gene="glnB"
                     /locus_tag="RH057_000049"
     CDS             complement(56716..57054)
                     /gene="glnB"
                     /locus_tag="RH057_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HDV3126474.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(57115..58449)
                     /gene="glrR"
                     /locus_tag="RH057_000050"
     CDS             complement(57115..58449)
                     /gene="glrR"
                     /locus_tag="RH057_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HDV3126475.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDIDVRIISAT
                     HRDLPKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNQFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(58439..59152)
                     /gene="qseG"
                     /locus_tag="RH057_000051"
     CDS             complement(58439..59152)
                     /gene="qseG"
                     /locus_tag="RH057_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HDV3126476.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQSHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIDALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYGKLQQASDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHEGEKPAPSTHEVTPDEP"
     gene            complement(59317..60744)
                     /gene="qseE"
                     /locus_tag="RH057_000052"
     CDS             complement(59317..60744)
                     /gene="qseE"
                     /locus_tag="RH057_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HDV3126477.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFSGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSRNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(61320..65207)
                     /gene="purL"
                     /locus_tag="RH057_000053"
                     /gene_synonym="purI"
     CDS             complement(61320..65207)
                     /gene="purL"
                     /locus_tag="RH057_000053"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HDV3126478.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFALDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWVIDGEQQPKSLFKMIKNTFETTPDYVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYDFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILNDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALAREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAAGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLSTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVAQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIATLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFHRAGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITSITTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            65465..67021
                     /gene="mltF"
                     /locus_tag="RH057_000054"
     CDS             65465..67021
                     /gene="mltF"
                     /locus_tag="RH057_000054"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HDV3126479.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKDTKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(67018..67554)
                     /gene="tadA"
                     /locus_tag="RH057_000055"
     CDS             complement(67018..67554)
                     /gene="tadA"
                     /locus_tag="RH057_000055"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HDV3126480.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(67579..68214)
                     /gene="yfhb"
                     /locus_tag="RH057_000056"
     CDS             complement(67579..68214)
                     /gene="yfhb"
                     /locus_tag="RH057_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HDV3126481.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            68423..69271
                     /locus_tag="RH057_000057"
     CDS             68423..69271
                     /locus_tag="RH057_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV3126482.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAAVRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            69327..69587
                     /gene="yfhL"
                     /locus_tag="RH057_000058"
     CDS             69327..69587
                     /gene="yfhL"
                     /locus_tag="RH057_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HDV3126483.1"
                     /translation="MALLITKKCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            complement(69781..69861)
                     /gene="shoB"
                     /locus_tag="RH057_000059"
     CDS             complement(69781..69861)
                     /gene="shoB"
                     /locus_tag="RH057_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HDV3126484.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(70281..70661)
                     /locus_tag="RH057_000060"
     CDS             complement(70281..70661)
                     /locus_tag="RH057_000060"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HDV3126485.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            complement(70661..71392)
                     /gene="pdxJ"
                     /locus_tag="RH057_000061"
     CDS             complement(70661..71392)
                     /gene="pdxJ"
                     /locus_tag="RH057_000061"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HDV3126486.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQRDKMRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELARIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            complement(71404..72132)
                     /gene="recO"
                     /locus_tag="RH057_000062"
     CDS             complement(71404..72132)
                     /gene="recO"
                     /locus_tag="RH057_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HDV3126487.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGATGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDD
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            complement(72144..73049)
                     /gene="era"
                     /locus_tag="RH057_000063"
     CDS             complement(72144..73049)
                     /gene="era"
                     /locus_tag="RH057_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HDV3126488.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLRDGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGYDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            complement(73046..73726)
                     /gene="rnc"
                     /locus_tag="RH057_000064"
     CDS             complement(73046..73726)
                     /gene="rnc"
                     /locus_tag="RH057_000064"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HDV3126489.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            complement(73999..74973)
                     /gene="lepB"
                     /locus_tag="RH057_000065"
     CDS             complement(73999..74973)
                     /gene="lepB"
                     /locus_tag="RH057_000065"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HDV3126490.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            complement(74989..76788)
                     /gene="lepA"
                     /locus_tag="RH057_000066"
     CDS             complement(74989..76788)
                     /gene="lepA"
                     /locus_tag="RH057_000066"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HDV3126491.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            complement(76986..77465)
                     /gene="rseC"
                     /locus_tag="RH057_000067"
     CDS             complement(76986..77465)
                     /gene="rseC"
                     /locus_tag="RH057_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HDV3126492.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAILGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            complement(77462..78418)
                     /gene="rseB"
                     /locus_tag="RH057_000068"
     CDS             complement(77462..78418)
                     /gene="rseB"
                     /locus_tag="RH057_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HDV3126493.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DIESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            complement(78418..79068)
                     /gene="rseA"
                     /locus_tag="RH057_000069"
     CDS             complement(78418..79068)
                     /gene="rseA"
                     /locus_tag="RH057_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HDV3126494.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQPATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            complement(79101..79676)
                     /gene="rpoE"
                     /locus_tag="RH057_000070"
     CDS             complement(79101..79676)
                     /gene="rpoE"
                     /locus_tag="RH057_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HDV3126495.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            complement(79673..79828)
                     /gene="rseD"
                     /locus_tag="RH057_000071"
     CDS             complement(79673..79828)
                     /gene="rseD"
                     /locus_tag="RH057_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HDV3126496.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            80084..81706
                     /gene="nadB"
                     /locus_tag="RH057_000072"
     CDS             80084..81706
                     /gene="nadB"
                     /locus_tag="RH057_000072"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HDV3126497.1"
                     /translation="MNTLPEHSCDVLIIGSGAAGLSLALRLADQHQVIVLSKGPVTEG
                     STFYAQGGIAAVFDETDSIDSHVEDTLIAGAGICDRHAVEFVASNARSCVQWLIDQGV
                     LFDTHVQPNGEESYHLTREGGHSHRRILHAADATGREVQSTLVSKAQNHPNIRVLERS
                     NAVDLIVSDKIGLPGTRRVVGAWVWNRNKETVETCHAKAVVLATGGASKVYQYTTNPD
                     ISSGDGIAMAWRAGCRVANLEFNQFHPTALYHPQARNFLLTEALRGEGAYLKRPDGTR
                     FMPDFDERGELAPRDIVARAIDHEMKRLGADCMFLDISHKPADFIRQHFPMIYEKLLG
                     LGIDLTQEPIPIVPAAHYTCGGVMVDDHGRTDVEGLYAIGEVSYTGLHGANRMASNSL
                     LECLVYGWSAAEDITRRMPYAHGVSTLPPWDESRVENPDERVVIQHNWHELRLFMWDY
                     VGIVRTTKRLERALRRITMLQQEIDEYYAHFRVSNNLLELRNLVQVAELIVRCAMMRK
                     ESRGLHFTLDYPELLTHSGPSILSPGNHYINR"
     gene            complement(81691..82428)
                     /gene="trmN"
                     /locus_tag="RH057_000073"
     CDS             complement(81691..82428)
                     /gene="trmN"
                     /locus_tag="RH057_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HDV3126498.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTDDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            82560..83894
                     /gene="srmB"
                     /locus_tag="RH057_000074"
     CDS             82560..83894
                     /gene="srmB"
                     /locus_tag="RH057_000074"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HDV3126499.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKVKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            complement(83927..84808)
                     /locus_tag="RH057_000075"
     CDS             complement(83927..84808)
                     /locus_tag="RH057_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3126500.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQSLNCYVIRDALLNDEADVGVFYRVGNDDVLNRRELG
                     EQPLVLVASPQIADIDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPRFAVGNELASGELIELPFGEQTQTITAMCAHHAGKAVS
                     PAMHTFIQCVEECFVAG"
     gene            84911..85498
                     /gene="eamB"
                     /locus_tag="RH057_000076"
     CDS             84911..85498
                     /gene="eamB"
                     /locus_tag="RH057_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HDV3126501.1"
                     /translation="MTPTLLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLIYCAVRIFY"
     gene            complement(85553..85936)
                     /gene="grcA"
                     /locus_tag="RH057_000077"
     CDS             complement(85553..85936)
                     /gene="grcA"
                     /locus_tag="RH057_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HDV3126502.1"
                     /translation="MITGIQITKAANDDLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            86241..86930
                     /gene="ung"
                     /locus_tag="RH057_000078"
     CDS             86241..86930
                     /gene="ung"
                     /locus_tag="RH057_000078"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HDV3126503.1"
                     /translation="MANELTWHDVLAEEKKQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAESE"
     gene            complement(86978..88015)
                     /locus_tag="RH057_000079"
     CDS             complement(86978..88015)
                     /locus_tag="RH057_000079"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV3126504.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVSQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            88222..88641
                     /gene="trxC"
                     /locus_tag="RH057_000080"
     CDS             88222..88641
                     /gene="trxC"
                     /locus_tag="RH057_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HDV3126505.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            88710..89408
                     /gene="tapT"
                     /locus_tag="RH057_000081"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             88710..89408
                     /gene="tapT"
                     /locus_tag="RH057_000081"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HDV3126506.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTDPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAEGQYCTAEVAIALLDMAGDTGAAAGLGELFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            89440..92100
                     /gene="pat"
                     /locus_tag="RH057_000082"
     CDS             89440..92100
                     /gene="pat"
                     /locus_tag="RH057_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HDV3126507.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSHNGKLATLSEETCQKLRDALPGHV
                     AISNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQELIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RIHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDIAPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRDHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLAQREES"
     gene            92214..93569
                     /gene="pssA"
                     /locus_tag="RH057_000083"
     CDS             92214..93569
                     /gene="pssA"
                     /locus_tag="RH057_000083"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HDV3126508.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGIPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIREHTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            93615..93938
                     /locus_tag="RH057_000084"
     CDS             93615..93938
                     /locus_tag="RH057_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HDV3126509.1"
                     /translation="MRILFVCSLLLLSGCSHMANDSWSGQDKAQHFIASAMLSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(93935..95233)
                     /gene="kgtP"
                     /locus_tag="RH057_000085"
     CDS             complement(93935..95233)
                     /gene="kgtP"
                     /locus_tag="RH057_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HDV3126510.1"
                     /translation="MVESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
BASE COUNT        22779 a        26054 c        24030 g        22685 t
ORIGIN      
        1 ttgatgatct taaatgtctt atttttcgta atgtgtataa caaggaatag tgatgaaatt
       61 taaaaaatgt cttctgcctg tggcaatgtt agcgtcattc actctggcag gatgccagtc
      121 aaatgctgac gatcatgccg ccgatgttta tcaaaccgat caactgaata ccaaacaaga
      181 aactaaaacc gttaatatta tttccattct tcccgcaaaa gttgccgtag acaactccca
      241 aaataaacgg aacgcacaag ccttcggcgc gcttattggc gcagtcgctg gcggtgttat
      301 cggccacaac gtcggttctg gcagcaattc cggaacgacg gcaggggcag ttggcggcgg
      361 agctgtaggc gcggcagcgg gttctatggt gaatgataaa accttagtgg aaggtgtttc
      421 tttaacatat aaggaaggca ccaaagtgta tacctctacc caggtgggta aagagtgcca
      481 gtttacgaca ggtttagccg ttgttattac cacaacgtat aacgaaacgc gtattcagcc
      541 aaatactaaa tgtcctgaaa agagctaata atcaggagga gtcatgaaga aagtttttct
      601 ttgcgccatc ttggcctcct taagctatcc ggctatcgcc tcatcattgc aggatcaact
      661 ctctgctgta gcagaagctg aacagcaagg taaaaatgaa gagcaaaggc agcatgacga
      721 atgggtcgcg gagcgcaaca gggaaatcca gcaagagaag caacgtcgcg caaatgccca
      781 ggccgccgct aacaaaagag cggcaacggc agcagcaaat aagaaagctc gtcaggataa
      841 actggacgcc gaagcctctg cggacaaaaa acgcgatcaa agttatgaag atgagctacg
      901 cagcttagag attcagaaac aaaaactggc gctggcgaaa gaagaagccc gcgttaagcg
      961 agaaaacgaa tttatcgatc aggaactgaa gcacaaagct gcgcaaaccg atgtggtgca
     1021 atctgaagct gacgccaaca gaaatatgac tgaaggcggt cgcgatctga tgaaaagcgt
     1081 gggcaaagca gaagagaaca aatcggacag ctggtttaat taatcgatgt tagtaacttc
     1141 aagcctatga ttcttgaaga taaaaaaccc tctgtagtaa cagagggttt tgttcattca
     1201 tagtgcaggg attaaaatca ttcccactca atggtagctg gcggcttgcc gctgatgtca
     1261 tacaccacgc gggaaatacc gttcacttca ttgataatgc ggttggaaac gcgaccgagg
     1321 aaatcgtacg gcagatgcgc ccagtgtgcg gtcataaagt cgatggtttc gacagcacgc
     1381 agagagacaa cccagtcata cttacgacca tcgcccatta cgccaacgga acgtaccggc
     1441 aggaacacag tgaacgcctg gctgactttg tcgtacaggt ccgctttacg cagttcttca
     1501 atgaagatgg cgtcagcacg gcgcagcagg tcacagtact ctttcttcac ttcacccagc
     1561 acacgaacgc caaggcctgg tcccgggaac gggtgacggt acagcatgtc gtacggcagg
     1621 cccagctcca gaccaatctt acgcacttcg tctttgaaca gctctttcag cggttcaacc
     1681 aggcccatct tcatctcttt cggcaggccg cccacgttgt ggtgagattt gatgacgtgt
     1741 gctttaccgg ttgcagaagc cgcagattcg ataacgtcag ggtagatggt gccctgcgcc
     1801 agccacttca cgtcttccag tttcagcgct tcttcatcga atacttcaac gaaaacgcgc
     1861 ccgatgattt tacgttttgc ttccggatcg ttttcgccag ccagcgctga caggaagcga
     1921 tcttctgccg gtacgtgaac aatgttaaga ccaaagtgat cgccaaacat atccagaacc
     1981 tgctctgctt cgttgagacg cagcaggccg ttgtcgacga atacgcaagt caggttttta
     2041 ccgatagcgc ggtgcagcag cattgcggtt acggaggaat ccacaccacc agagaggccg
     2101 aggatgactt tatcgtcgcc gacctgctcg cggatgcgag ctacagcatc gtcgataatt
     2161 ttcgctggcg tccacagggc ttcacactgg cagatatcac gcacaaaacg ctccagcatg
     2221 cgcataccct ggcgggtgtg agtcacttcc gggtggaact gtacgccata gaagcgtttt
     2281 tcttcgttag ccataatggc aaacgggcag ctttcggtgc tggctacggt gacgaagtcg
     2341 gacgggatag cggtaacttt atcgccgtgg ctcatccaga catcgagcag cggttttccg
     2401 tctgcggtca gcgcatcttc gataccgcga accagtgcgc tgtcgtttac gacttcaacc
     2461 tgcgcgtagc caaattcacg ttcgttagag gcttcaacgt gaccgcccaa ctgcattgcc
     2521 atggtctgca tgccatagca aacgccgaat accggtacgc ctgcttcaaa gacatactgc
     2581 ggcgcacgcg ggctgttttc ttcggtggtg ctttccgggc cgccggaaag aataatgccg
     2641 cttggattga agtcacgaat ttgtgcttct gtcacatccc acgcccacag ttcgcagtaa
     2701 acacccagct cacgcacgcg gcgcgcaacc agttgagtgt actgagaacc gaagtccaga
     2761 atgaggatgc gatgcttatg aatgttttcc gtcattgacg cttattccga ggcaagtgaa
     2821 acagataata taaatcgccc gacatgaagt cgggcgaaga gaatcaggag cccagacggt
     2881 agttcgggga ctctttagta atggtcacgt cgtgaacgtg gctttcctga atgcccgcac
     2941 cgctgatacg tacaaactcc gctttagtac gcagttcgtc gatagtacca cagccggtca
     3001 gacccataca ggagcgcagg ccgcccatct gctggtgaat gatctctttc aggcgacctt
     3061 tataagctac gcgaccttcg ataccttccg gcaccagttt gtcggcagcg ttatcgctct
     3121 ggaaataacg gtcagaggaa cctttggaca tcgcgcccag ggaacccata ccacggtaag
     3181 atttgtaaga acggccctgg tagagttcga tttcacccgg agattcttca gtacccgcca
     3241 gcatggaacc taccatcacc gcgcttgcgc cagcggcgat agctttggcg atgtcgccgg
     3301 agaagcgaat accaccatct gcgataaccg gaataccggt gccttccagg gcttcaactg
     3361 cgtcagcaac ggcagtgatc tgcggaacac ctacgccagt aacgatacga gtcgtacaga
     3421 tagaaccagg gccgataccg actttaaccg cgctgcaacc agcttctgcc aaagcgcgtg
     3481 cacctgcagc tgttgccacg ttgccgccga tgatttgcag atccggatat ttagcacggg
     3541 tttcacggat acgctgcaaa acgccttctg agtgaccgtg ggaggagtcg atcagcagaa
     3601 cgtcaacgcc agcggcaacc agcgcgtcaa cacgctcttc gttacccgca cctgcgccaa
     3661 ctgcagcacc aacacgcaga cggccttgct cgtctttaca ggcgttcggt ttacgttccg
     3721 ctttctggaa gtctttcacg gtgatcatgc cgatcaggtg gaattcgtca tcaaccacca
     3781 gcgctttttc aacgcgtttt tcgtgcattt ttgccagcac cacttcacgg gcttcacctt
     3841 cacgcacggt gaccagacgc tctttcggcg tcatgtaaac gctaaccggc tggttcaggt
     3901 cggtaacaaa acgcacgtca cgaccggtga taatacccac cagttcgttt tcttcggtaa
     3961 cgaccggata gcccgcaaaa ccgttacgct cagtcagttc tttcacttcg cgcagcgtcg
     4021 tggttggcag aacagtctgc ggatcagtca ccacaccaga ttcgtgtttt ttcacacggc
     4081 gaacttcttc tgcctggcgt tcaatggaca tgtttttgtg gataaagccg ataccgcctt
     4141 cctgagccag agcaatagcc aggcgcgctt ccgttacggt atccattgct gcggaaagca
     4201 tagggatatt cagacgaata gttttcgtca gctgggtgct gaggtcagca gtattcggca
     4261 gaacggtaga atgagcagga acgaggagaa cgtcgtcaaa cgtcagagct tctttagcga
     4321 tacgtagcat gggcaatatc tcgaccagag tggttaataa atattgccgc ggcattatac
     4381 agagcgtaac cgattgcatc tacccctttt tgcaaaaaat gcttgctatc cccgaagggc
     4441 gggttactat cgactgaata acctgctgat ttagaatttg atctcgctca catgttacct
     4501 tctcaatccc ctgcaatttt taccgttagt cgcctgaatc aaacggttcg tctgctgctt
     4561 gagcatgaga tgggacaggt ttggatcagc ggcgaaatct ctaatttcac acaaccggct
     4621 tccggtcact ggtactttac actcaaagac gacaccgccc aggtacgctg cgcgatgttc
     4681 cgcaacagca accgccgggt gaccttccgt ccacagcatg gacaacaagt tttagttcgc
     4741 gccaatatta cgctctacga gccgcgcggt gactaccaga taatcgttga gagtatgcag
     4801 ccggccggtg aagggctgct gcaacagaag tacgaacagc tcaaagcgaa gttgcaggct
     4861 gaaggtttgt tcgatcagca atacaaaaaa ccacttccct cccctgcgca ttgcgttggt
     4921 gtgatcacct caaaaaccgg tgctgcgcta catgatattt tgcatgtgtt aaaacgtcgc
     4981 gatccttctc taccggtgat catctacccc accgccgttc agggcgatga cgcaccgggg
     5041 caaattgttc gcgccattga actggcgaat cagcgcaatg agtgcgacgt gttgatcgtt
     5101 gggcgcggcg gcggttcgct ggaagattta tggagtttta acgacgaacg cgtagcgcgg
     5161 gcgatttttg ccagccgcat tccggtcgtc agcgccgtcg ggcatgagac ggatgtgacc
     5221 attgccgatt ttgttgccga tctgcgtgcg ccaacgccgt ctgccgccgc tgaagtagtg
     5281 agccgtaatc agcaagagtt actgcgccag gtgcaatcga cccgtcaacg gctggagatg
     5341 gcgatggatt attatctcgc caaccgcacg cgtcgcttta cgcagatcca tcaccgatta
     5401 cagcaacagc atccgcagct ccggctggca cgccagcaaa ccatgcttga gcgcctgcaa
     5461 aagcggatga gctttgcgct ggaaaatcaa cttaagcgta ccgggcaaca gcagcagcgg
     5521 ttaacacagc ggctgaatca gcaaaatcca cagccgaaga ttcatcgcgc gcaaacgcgc
     5581 attcagcaac tggaatatcg tttagcagaa accctgcgcg tacagcttag cgccacgcgt
     5641 gaacgtttcg gtaatgcagt aacgcacctc gaagccgtaa gcccactgtc aacgctggcg
     5701 cgtggataca gcgttactac tgctactgac ggcaatgtac tgaaaaaagt gaagcaagtt
     5761 aaagcgggtg aaatgctaac cacacgtctg gaagacggct ggatagaaag tgaagttaaa
     5821 aacatccagc cagtaaaaaa atcgcgtaaa aaggtgcatt aagccagcac gaattcaacg
     5881 cgttttttcg atatcaagcc atgcccgtgc tgacagaagt aatctactgc accgcaggct
     5941 tttaacacct gcaacggttg atggcagtct ggacaaagag ctttcatttc aataatttcc
     6001 ccgcaggatg ggcaacgcgc atggccgtta tcctgatcaa gcacatgctg acattgtgga
     6061 caatgcagtt ccatatgaat tcctccggta gccatccctc tacattcata gagggaatgg
     6121 cagataaaat acttacggat aattatttat ttttcttgat gtgcttcatc agacgcttac
     6181 gtttacgcat ctgggttggc gtcagggtgt tacgcttgtt cgcatacggg ttttcccctt
     6241 ctttgaactg aatacgaatc ggcgatccca ttacgtccag cgatttgcgg aagtagttca
     6301 tcaagtagcg cttgtaggaa tcaggcaggt ctttcacctg attaccgtga atcaccacaa
     6361 tcggcgggtt ataaccacca gcgtgggcat atttcagctt cacacgacga ccgcgtacca
     6421 gcggcggttg gtgatcttca acagccatcg tcatgatgcg cgtcagcata gaggtcccca
     6481 cacgacgggt ggagctgtca tacgcttcac gtactgattc aaacaagtta ccaacaccac
     6541 tgccgtgcaa ggcagagata aagtgcacac gagcaaaatc gataaagccc agacggaagt
     6601 ccagcgtttc tttcacctgc tctttcactt cctggctcag gccatcccac ttattcacca
     6661 caatgacaag tgagcgccca ctattgagaa taaagcccag cagcgagaga tcctgatcgg
     6721 aaataccttc gcgcgcatca atcactaaca tcaccacgtt ggcatcttca atggcctgca
     6781 acgttttgat tacggagaat ttctctacag catcggtgat tttgccgcgt ttacgtacgc
     6841 cagcggtgtc aatgagcaca tactcacgtc catcgcgttc cattggaatg tagatactgt
     6901 cacgcgtcgt gccaggcatg tcataaacaa caacgcgctc ttcaccaaga atacggttag
     6961 tgagtgtaga cttacctacg ttcggacgac ccacaatcgc cagtttgatc ggcagacttt
     7021 gcgggtcgaa gtcgtcttcc tcttcttctt cgccgttctc ttccgcttca aattgcgccc
     7081 agtattcagc gtcttcgtcg acttcctctt gcggtgcgag atcttccatc cacggcagca
     7141 gcacatgctc cagcagactt aatacgccac gaccgtgaga cgcggcgatc gggtagattt
     7201 cacctaagcc aagcgagtag aaatcaacca ctgcctgatc gggatccaga ccgtcagttt
     7261 tgtttgccac caggaaggtc ggtttttcac gggagcgcag atgtttggca atcgcttcat
     7321 ctgccggcat caggcccgcg cgcgcatcca ccataaacag tacgacgtcc gcttcttcaa
     7381 tcgccagcag cgactgttcc gccatgcggg tttctacacc gtcttctgtg ccatcaatcc
     7441 cgccggtatc aatacagata aactcacggc cttcaatttc cgcacgaccg tacttacggt
     7501 cacgagtcag acccgggaaa tccgcaacca gcgcatctcg ggtgcgagtt agacggttaa
     7561 ataacgtgga ttttcctacg ttagggcgcc cgacaagcgc gaccacaggt accatgttta
     7621 aagcctcatt tttataaatc atcagacaac gcacgctata ttcgcgtcgt tgttaaaaac
     7681 aggaaaacgg cccctgtcca ggagccgttt tcaaagtgaa cgacagagac gattaacgtg
     7741 taatagagta cacggttccg tcttttgcct ggatcagcag tttgccgtca gcggcaaccg
     7801 gttcagtctg gaaaccggaa ctatcaactt tttgctgggc aacgaaacga ccatcttcga
     7861 cgttaatcca gtgcagataa ccttcactgt cacctaccac caggttgcca ttatacagca
     7921 ccggagaagt cagcaggcga tgcaacagat cgctttgtgt ccacagcgta acgccgccat
     7981 caatggtcaa cgccatcacc cggtcatttt gatcgaccag atagatgcga ttgccgtcga
     8041 cgatgaaatc attcaccgaa cccagttcgc gtttccacat aatctgacca ctgcgcagat
     8101 caagcgccgt caggttacca ttataggcca gcgcgaaaac aacgccgtta acaacgacgg
     8161 gagtcgtgtc aacatcgctc agacggtcaa tttcggtaga accggtcgcc tgggaaatac
     8221 gctgctgcca aatcatctgg ccctgttcca tcagcactgc gctgacgcga ccattatcgc
     8281 cacccacgac ggccgcacca aaagccgttg ccggcgcaga ctcgccacgc aaagagagcg
     8341 aaggcatatc gaggttaact gtccatttga cagcgccgtc agcctcgttc agcgcttgta
     8401 actgaccgtt actggtgtgg attaacacca gaccgtcgct gaccaccggg cgcgaaagtg
     8461 cttcacccgc gactttagtt tgccatgcca cagtaccatc gctggtattc agcgcgtaaa
     8521 cctgcgcctt ttcgctgcca atgtagacat gcccaccaga cacggtcaga ccgccagaaa
     8581 gtaatgcagg ctctttagag aaccagccat ctttctcggc caggctgaca gaccagattt
     8641 ctttgccatc atccgcattc agcgctttta ccaaaccagc gcggtccgct gcatagacaa
     8701 cgttgtccgc cagtgccgga tgaagattgg aatagaagtt gccaatgccg ctaccaacgg
     8761 aagtgctcca cgccgtggtc ggcgtaaact ggttttcaac ggttggcaat ggggacatct
     8821 ttaccacatc ttcttcgctg ttaaacagcg aacagccgct taaaagggta acggaaagca
     8881 gtcctggcag cagtaattta cgcaattgca tcgggtccct ctcagatgga caaattatta
     8941 attttcatct gcatcatttc gctcagtgcc ggagtaacat cgcttttcac gcctgcttcc
     9001 catgcactac gcgcaccttg cttatcacct ttgctcagca atgcttcacc acgcaggtcg
     9061 gcaacaatgg cagcccaccc ttcacctttg atggtatcaa gggttttcag cgcggcatca
     9121 gcctgcttga gctgtacctg aacgcgagca agacgcagat ttatcacggc tttgagattt
     9181 tcatcgctcg tgtctgccag cccctgttgt aactgggcgg cagctttctc tagttcattt
     9241 ttgtcaacaa attgctgcgc aagttccaaa gaagccagcg caccataagt atttttattt
     9301 tcagcagcaa atttttccgc cgccgggatg ctatccggtt tgccttcgct cactgcggta
     9361 accgcatttt gataggcaag agaagcggag cgtgcagaat caacctgatg gctgttccag
     9421 tagcgccagc caatcagtgc gccaacgccc aaaatcaccc caacagccag tgctttgcca
     9481 ttttcagcaa aaaagcgttt aaccgcttct acctggtcgt tttcgttctc gtaaatttcc
     9541 acgctgtcct tctccttcct taacccagta acgtgcgcaa atgcgcggct acgctatcct
     9601 gcgcaactgc cgtttgctca ccagagcgca aatccttcac tactgctgtg ccgttagcca
     9661 cttcagactc acccagcacc acagcaacgc gggcacccca tttatcagca cgggcaaact
     9721 gtttcttaaa gttgccgccg ccgtggttgg tcatcaattt cacgcccggt aattcatcac
     9781 gcagacgctc agctaatgcc atagccgcag attgtgtatc agcacctgaa gccaccaggt
     9841 atatatcgac aacaggatcg gctttaaatt ccggattaac ggcctgaact aacaatacaa
     9901 gacgttcgag gcccatcgca aaaccgactg ccggtgttgc acgaccgccc agttgttcca
     9961 ccagaccgtc ataacgaccg cctgcacaca cggtgccctg ggagccgaga ctgttagtca
    10021 cccactcgaa aacggtacgg ttatagtaat ccagaccacg caccagacgc tggtttacgg
    10081 tgtaagcgat ccccgcgctc tcaagcagtt tgcacagacc ggcaaaatgc tcacgagatt
    10141 cctcgtccag ataatcacct aatgccggag cgtcgttgag aagcgcctgc acttccggat
    10201 tttttgaatc cagcacgcgc agcgggttag tgtacatgcg gcgtttgcag tcttcgtcca
    10261 gcttttcttt atgctgctca aggaatgcca ccagcgcatc gcggtaattg gcgcgtgctt
    10321 ccagcgaacc gatagaattc agctcaagag ttacgtgttc ggaaataccc agcgcgcgcc
    10381 accagcgggc ggtgagcata atcagttcag cgtcgatatc cggaccttgc agaccgaaaa
    10441 cttcgcagcc caactgatgg aactgacgat aacgcccttt ctgcggacgc tcgtgacgga
    10501 acatcggccc gatataccac agacgctgtt cctgattgta cagaagacca tgctcgatgc
    10561 cggcgcgtac acagcccgcc gtcccttcag ggcgcagagt caggctgtcg ccattgcgat
    10621 cctcaaaggt gtacatctct ttttcaacca cgtcggtgac ttcaccaatc gcacgtttga
    10681 atagcggggt ctgctctaca atcggcaagc ggatttcact gtaaccgtag ctgccgagca
    10741 cgtttttcag tgtgccttca atgcgctgcc agatggccgt ttcgccaggc aggtaatcgt
    10801 tcatgccgcg aatggcttga atgttttttg ccacgtttat tctctttctg aatataaaaa
    10861 tgaaccctca acgcttccct caatgtttgg ggagccatgc gggttcaatc atacacggga
    10921 agcgaggcgc ttcccatcac gttattattt ttcgacctgc tgaacgtcaa ttcgacgcgc
    10981 ttcgtccagc tgactggctt tcgcacgaat gcgtgcttcc agctggtcga tcatatcgtt
    11041 gttgtccaga cggtctttgc gcacgccatc ttcatagagg ccgcttttct tgttgccgcc
    11101 ggtgacgccg agtgtagaaa ccagcgcctc acctgggcca ttcaccacgc agccgataat
    11161 cgaaacgtcc atcggagtga tgatatcttc caggcgttgc tccagcgcgt taaccgtacc
    11221 gataacatca aactcctgac gcgaacaggt cggacaggcg atgaagttga tacctcgcga
    11281 acggatacgc agcgatttca aaatatcgaa accgactttg atctcttcga ccggatcggc
    11341 cgccagtgat acgcgcagcg tgtcgccgat gccttcagac agcagcagac ctaaaccaat
    11401 ggcggatttt actgccccgc tgcgcgcgcc acccgcttcg gtgatcccca gatgcagcgg
    11461 ctgatcgatt tgttttgcca gcaaacgata agactcaaca gcgaggaaga cgtcagacgc
    11521 tttcacgctg actttgaact gatcgaagtt caggcgatcg agatgatcaa catggcgcat
    11581 ggcggattcc agcaacgcct gcggcgtcgg ttcgccatac ttttcttgca gatctttttc
    11641 cagcgatccg gcgttaacgc caatgcggat cggaatgttt ttatcgcgcg cacagtcaac
    11701 taccatgcga atacgctctt cattaccgat attgccaggg ttaatacgca gacaatcgac
    11761 gccgtattcc gctactttca gcgcaatgcg atagtcgaag tggatgtcag ccaccagcgg
    11821 cacgttaacc cgctgtttga tgagtttgaa cgcttctgcc gcgtccatcg tcggtacgga
    11881 gacacggacg atatcagcgc caacgcgttc cagcgccttg atttgattga ccgttgcttc
    11941 gacatctgtc gtacgcgtat tggtcatgga ctgtacggcg atgggagcac catcgccaat
    12001 cggcacattc ccaacgtaaa tacgtgttga ttttctacgt tgaattggag cctggttatg
    12061 catgaaaaat ctcccgcgtt acccgtctgt tactgcgccg gtgattgttc ggcattgagg
    12121 gtcagacgcg caacctggtt agttctgata aaacgactca gatcgacagg tttcccttga
    12181 tactggatct gtactgcggc tggcgcacca attttcagtt tgtacggtgc ctggccggtt
    12241 aagttcaaat taccgtcttt acgctgcata ccgctaaaca attttttacc ggtagcatca
    12301 gtaacctcca gccagcaatc ggcagtaaag ttcatcacca gcgcattcgg atcagccgcc
    12361 ggcgtagtca cgcctgcctg atcggttggc aacggcgcag cgccatctgg cgttgttgtt
    12421 gccgttggtg ccggggtcgc ggcggtatca acatttgcct gcgaaggcga aacaaccgca
    12481 ttctgttgcg gatcaacagc tggtgctggc gcagttacgg caggtgtttg cgtgttggtt
    12541 gcggtagtat ccacagacgc tggcggcgtg gacgttgtag ccgggtctgt agttgtcgac
    12601 gtatttaacg gaacactctg cccctgctcg ctattactgc tcagttccgc cgaagattga
    12661 tcggccatag tggtgatctc ttcctgctga gctttgtggt cttgccacca ccaggcaccg
    12721 ctcaggccga taaccacaaa caacaccagc caggtgaagg tcatcagcca gccgtcacgt
    12781 tttttgcggc gtttaccgag ggaaaaactc tgcatcggcg caactttcgc agcccgtagt
    12841 ggagcctgct tttccagccc tggcagcagt tcttcttctg gaatatgtac cagacgcgca
    12901 taagagcgga tatatccgcg caggaatgtt gaagcaagat cggcgggtgc cttatcttct
    12961 tcaatgtcgc gtaccgtgga aaccttcagg caaagtcgct cggcaacggc ctgctgacta
    13021 agtcctagtt gttcgcgagc attacgcagg cgagcgccgg tagtaagtgc ttcattttgg
    13081 tcgtgcgtgg cttcagtatt cattcgctgc aggtacgttt aaatgagaat tgagatgccg
    13141 gtgaaccatc atgctcaccc acaccgcgaa acatccgtta agttaaccgt tatcatacag
    13201 tataagaccg tctgtgtgct cgtgacaaag cccacattca ctaaggctaa cttactgttg
    13261 catcgttaca tactgcctta aagtcagcaa aaacgcaccg ttaatattga ccagacaatt
    13321 gcaacttaat acttcattca taaaagatta cgccacggta cataaagtaa ccgtggcgta
    13381 atggctatca gaccgcttta atgtcgatgg cttcaccctg catccgttta cgcagggtac
    13441 gtttcgtacg gtcgataaca tcgcccgcca actgaccaca ggcagcatcg atatcatcac
    13501 cacgggtttt acgcacaata gtggtgaaac cgtagctcat cagcactttt gagaaacggt
    13561 cgatacggct gttcgagctg cgtccatacg gcgcgcccgg gaaggggttc cacgggatca
    13621 ggttgatctt acacggcgta tctttcagca gttccgccag ttggtgcgcg tgttcagtgc
    13681 cgtcgttaac gtggtcaagc atcacatact caatagtgac acggccctga ttggcgttgg
    13741 atttctccag ataacggcgc accgcagcaa ggaacgtttc gatattgtac tttttgttga
    13801 tcggcacaat ttcgtcacga atttcgtcgt tcggcgcgtg cagggaaatt gccagtgcaa
    13861 cgtcgatcat atcgcccagt ttatccagcg ccggaactac accggaagta gaaagcgtga
    13921 cgcgacgttt agacaggcca aaaccgaaat catcaagcat gatttccatc gccggaacga
    13981 cgttgttcag gttgagcagc ggctcgccca tgcccatcat cactacgtta gtgattgggc
    14041 gctgaccggt gacttttgct gcgccgacga ttttcgccgc acgccacacc tggccaataa
    14101 tttccgatac ccgcaggttg cggttaaagc cctgctgggc ggtggaacag aatttacact
    14161 ccagcgcaca ccccacctgc gaagagacgc agagcgtggc acggtcgtct tccgggatat
    14221 acaccgtttc gacgcgctga tcgccaacgg cgatcgccca tttaatggtg ccgtcggatg
    14281 aacgctgttc ttcaaccact tccggtgcgc ggatctccgc cacctctttc agtttgccgc
    14341 gcaacacttt gttgatgtcg gtcatctcat caaagttgtc gcagcaatag tgatacatcc
    14401 acttcatcac ctgatcggcg cggaagggtt tttcacctaa atctttaaaa aactcccgca
    14461 tctgctgacg gttgagatcc agcaggttga tttttccatc tttcgtggtg acgttttcag
    14521 gtgtgactaa ttgttcagac atatgctatt ccggcctcgt tattacacgt tatggcccct
    14581 ggagggttga aaaaagaaac gccccggtga gcggctgctc gtccgggggc gctgcattgt
    14641 acaaattctg gcgcacggat gccacgtttg cacgcggcat ttacgaaatt attaacgggt
    14701 gcgcgggcac acttcgcctt cgccaaagaa ataagcgatt tcgcgagcgg cagattcgac
    14761 ggaatcagaa ccgtgggtac cgttttcggt caggctgtca gcgtaatcag cgcgcagagt
    14821 accagccagt gcgtttgccg gattggtcgc gcccagcaga tcgcggtgac gctgcacggc
    14881 gttttcacct tccagcacgg aaaccacgat cgggccagag gtcatgaatt caaccagacc
    14941 atcaaagaac ggttttccat cgtgttcagc ataaaagcca cgtgcctgtt caacggtcag
    15001 gtgcagcatt ttggtgccga caattttgaa ccctgcagct tcaaagcgcg caaagatatt
    15061 accaatgacg ttttttgcta ccgcgttcgg tttgatgatg gaaaaagtac gttcaatagc
    15121 catttttacc tctgtaaatt gttctgttgt tgtctgtacc agcgtacgag atggcgcgga
    15181 ttataatgag caacagggcc gttgactatt gatgaaggta acattttttt aaaataaaat
    15241 gagttttagc aacaatcagg ggcaaaacag actattgcat gacaaatttc actgtcgcga
    15301 tttgccccac atcatccatc accagcaatt gataatcacc tttatccgtc aaatgcaggg
    15361 tcacgttgcg cccgcgttca gttaacggtt cgccattcag aaaccaccag cgttcacctg
    15421 cccctccact tgattgcaac ggcaaagtcg cttccgctgc gcccggtaaa cgtttaataa
    15481 tcgcgccatc gcgaacgcct gtcagctgca gagggagctt ggcatcgtga ccgtatggcg
    15541 ggcaaattgt cgaggccggt ggtaagcgca cagcgcgacg ttctgatgcg ggcagccagg
    15601 gttccagcgg tagcggccag acatttatca tttcttgtcg cgcttgcggg caatcagcgg
    15661 caacacgttt gccattttca tccagccaga tggggaaacg aatgccatta atgccttcct
    15721 gctccggcag taatagagtt ggcggctgac tcccgtccag cagccaggtt gccaggcggc
    15781 ggcgacagtt accgtcacct tccggcaaag actgtccgcc cggccagcag ataacgccac
    15841 gagtgactga gttcgggcgc gggtcttccg gcagattcgc actgcgcgac agtaagatat
    15901 tattgacctg attcaacaat ggtacggcac tggcaaagcc aaactgacca acaacgggcg
    15961 tgccgtccgg tctgccagtc cagatcccaa tgacatagcg agcgttaacc ccaatcgccc
    16021 aggcgtcacg atagccatag ctggtgcccg ttttccatgc cagtggggcg acgcgcggca
    16081 aggcactatc cggcaagggt ttcgcttcat cagccataat ccggcgaatg atccacgccg
    16141 cccccgacga cattaaaggc cgttcaagca gcggatcgtc aggctgtaag cgcaatttgc
    16201 ctgccttgcc gtggcgagca aacgcggtat acgctgccgc catatcttcc agttttgcac
    16261 cagcgccgcc gagaatgagt gaaagattcg gcgcagcacc gttgggcaaa tataacggca
    16321 atccaacatt gcgtaacttt gccgcaaacc gtttcggtcc ataggcttcc agcacctgca
    16381 cagcaggtaa gttcagcgag cgcaccagcg cctcgctcat gctgatcggg ccatgaaaac
    16441 cgctatcaaa gttacctggt cgataatcac cggtgcgccg ggggacgtct tgcagcagtg
    16501 atgccgggtg gatcaagcct tcatccagcg ccagaccata aacaaacggt ttgagcaccg
    16561 atcctggcga tcggatcgca ttgaccatat caacatgacc aaagcgtgaa tcatcgttga
    16621 gatcaaccga tcccacccag ccgcgaacac gcatatcggt atgatcaacc acgatcatcg
    16681 ccagtgagct gcgcggtggc aaccgccctt tccagttttg cgccagttct tccagacgtc
    16741 gttgaagacc ggcatccagc gtagtaacga ttttgtcgct tttgctttta ccgagcatca
    16801 tgcgcgaaaa cagcggtgcc agttgcggca tttgtcgggg tgccagccag atgggttctt
    16861 cccttgactc ttttacctgc tcacgggacc acacaccttg cacggccatc cgttcgagca
    16921 ctttattacg cgcggcttcg gcacgctccg gccaacgatc cgggcgaaga cggctgggcg
    16981 cttgcggcaa aaccgccagc attgccgcct cggaatagct taaattcgca ggcgactttc
    17041 cgagataagc ccaacttgcc gcaccgatcc cctgcaacgt accgccaaac ggagcgcggt
    17101 taagatacaa ggtcagaatt tcacgcttag acagatgcca ttccagttgc aacgcgcgcc
    17161 agagctggcg aattttgccg ccaaatgttt tggggtgagg atcaagcaga cgagcaacct
    17221 gcatagtgag cgtgctgcca ccggaaataa cccgtcccga agtgagatct tgccatgctg
    17281 cgcgcgccac cgagaatgga ttcacccccg gatgcttcca gaaccagcga tcttcataat
    17341 tgatcagcgc ttcaaggtaa cgtggagaaa catcttcgat tgttaccgga taacgccaga
    17401 tgccgtcagc atcggcgaag cgccatagcg gcgtaccatc ctgcgccacg accactcgtg
    17461 cgggattgac ttcatgcagc ggtagaggcc agagtttatc tgctccccac gctgcaagaa
    17521 aaaggagaaa tggcgcagct gccagcgtta tccagcagcc gcgtttggtt aacaagcgag
    17581 gcatttacgg tcggacaatc agcagatctt cagccgcgcc ggtcgcccgc cattggggaa
    17641 catacattga ttccaccatc ggttgcggta cctgatacgt tcccggcgtc accgcccgcg
    17701 ccagatacac cagcgttacc ggttggtatt catcaacggc aaccgccgcc acaaagcgat
    17761 cgtcacggaa ctcaatgtgc ttaatgctcg cctgctgcat ctggttcagt aagttttgca
    17821 cttcgccacc actttgctcc aggctggcgc taccgttcgc cagattctga ttttccagtt
    17881 ccagacccgc aggcagcaga tccacgacta acgcatccgg cacgctgtta ctggctttta
    17941 cctgcaacca gaccagcacc agatcgccgc tacgtaacga gtccagcgat ttgctcttac
    18001 catcagtacc aagaatatga cgctcgattt gcagcacatt gttcgccggt aaaggtgcgg
    18061 attgcggata accgctggca tccatacgca accataacgg ctgatcgcca ctgtttctca
    18121 cctgcaaggt ggcaagttga tcgctattca gattgctgtt tagcgttttc tcgcctgtca
    18181 gcggctcagc tgagaaagag gtttgcgcct gccatttacc cggtaaatcc tgaatcgtac
    18241 gggcagccag gaacaacgcg ttactttcct gcgtcgatag ccagcgttca ccaaacgcct
    18301 gctgcgaaag tgtgttcagc aaagtgtact gctcatcggg tagcagttta ttttcttcca
    18361 gcaaggagag cattaacgcg ttgtcgcgca gtggactacc gtaatcaccc agccatatcc
    18421 gctcatcact atttctcggc gttttcagcg ccagcacaat cgcttcttca ccacgcgttg
    18481 catcacccat ggttttcagc gcaacgccaa gttgcagcag cggtaaacca gaagcggcat
    18541 ctgcacgatg ctcccagatt tcacgcagcg cacccagtgg tgctttttgc tgacgggcca
    18601 gcaccagcgc agcgtaagac tgaacggcga atttactggc tttgagatta tccgcgtacg
    18661 ggatcgacat catgcccgga tcttgtaaat agcgcagcag acgctcatta ccccggttaa
    18721 tggcgtctgt cggcacgctg taaccctgtt cgcctgcgcg gaccaggaaa tccatcacgt
    18781 aagccgtcag ccagtactct tcgtcaccgt ttttatccca cagcgcaaag ccgccgttat
    18841 cacgttgcat ttgcagcaaa cgggaaatgc cgatatcgac cgatgcacgg cgtttctcat
    18901 cactgtcgcc tttgatgccc aacgcctgca gttgggctgc gttggtataa agtgacggga
    18961 acaggccgct ggcggtttgc tcaagacagc cgtacggata cgcttttaac tctttgatat
    19021 aacgtgcaat gttcagcggt ggtttgccgc tcaacaacaa ttgcccttcc agcgtaacag
    19081 gcgagaagtt ttgcaatcca tccgccggaa tcgcccatgt ctcaccaggc tgtaacgccg
    19141 taccgtaatt aaccgtttgt gccgggaacg ccggacggac gccgattttc cactgcttat
    19201 gctgatcggc aacggtttca cccggtaacg ctaacccgct aatggtggcc tgaatttctc
    19261 catcgccata acccggcaat gctcgcaccg ggataaacag cgtagtacgc acacctggcg
    19321 ctaattcaac ggctgcgggt gaatcgctga ccagttcaag caaaccactg gcggtcaggg
    19381 caacgttcag tttttgcggt ttatcggtaa gattagtgat atccagcgtc agacgcgagg
    19441 tatcgccact cgccataaag cgcggcatgt tcagttcagc aatcaccggt gcggcaacta
    19501 tcactttgct ttcgttgcta ccgaaatcat ctgccgtcca ggcttgcgcc atgacgcgca
    19561 attcaccgtt aaaatcgcca atcggcagtg taaccgagcc ttcgccctgt tcgttgagcg
    19621 ttaccggcag cgcctgctgc gcgacaatat tgacgtgatt gaccggcggt ttaccaccac
    19681 gtttcagctc atcaccatcg ccaccgaaac gcagagctgc cagacgcccc tgaccttcaa
    19741 taacctgacc gtaaatatcg taaatgtctg cgccatagcg tttctgacca aagaacgcct
    19801 gccacggatc tggcgtgacg tagtcagtaa tattcagcac accactatca acggcagaca
    19861 ccagcacatt cacctgttta ggcttctcgc catttttagt gctggcttta attttcacgg
    19921 ttaatggttg attgggacgc atttttgctg gtgtttccag cgccagatcg aggcgacggt
    19981 tttcatcgcc aagcggcaga tgcaacacac caaccgcgcg ttttggcgtc gcggagcgag
    20041 atttatcgcc aggacgtacc accagcgtac ttaaatacag atcatgacga ttccaggttt
    20101 tatcgaccgg aatcgtcaga tccagccctt gagcccgaac atcaatctct tgccaccaca
    20161 gcggcccttc actggactcg accatcgcat aacctttacc cgccgttggc gcggcgatat
    20221 gcaacttaat ggtgtcgcca gggcgataac tggctttatc cagtttcagc gtgacacggt
    20281 cgggtcgcac tgcgccgcta ccgtcgctgt tgtcctgcca gctatagcca gcccagaaac
    20341 gaacactact gaccgcttca ttcggcgctt tgacttccag acgataagcg ccccactcta
    20401 ccggaaaact gaccttgccg gtttcgtccg ctttcagatc cagagtttgt tcattttcga
    20461 tcagatcttt ttgatcaaac tgtgactgcc agccttcatc ttctgaccag ttccagtagt
    20521 aatcgcggcg ttcgcgaatc aggcgcacct gcaagcccga cacggctttt ttcacgcctt
    20581 gcgcatcgct ataaacgatg tcaaaagcgg cgttactgcc ttcatcaaca atgggctgtt
    20641 tcaccgtgct gtcagtacga taatcgtaaa ccgatttcga ggcgaactgc ggacggatcc
    20701 ccggcaatgc atcggcaggc cagatagcct gctcagcgcg gcgcgtcacc gggcgaccgc
    20761 ccgattccag caaactaccc tggaaaataa cctgtaatgg ggaatgcgtt tccttccact
    20821 ggctttctgt agaaacttcg ccgcgccctt tatcatccag cgtcaactga acttcatcca
    20881 gcgtgcggga aagattttcg gcagctatat cgccgaattc aaaaccaggt aaggctgaca
    20941 cagcttcacg cagtgggcgc aggaaaagtt gcccttgcaa agtattacca ttagcaggtg
    21001 caccatacag gtagtacccc accacggaga atttcacttc atctttcggc gttagcgggg
    21061 ttttctcacc ggtcagattc agcgccatgc gctctggcat aaaatcttcg acgtggaaat
    21121 cccacatccg atactggtta tcgcccgtgt tagcgcgaat atgccacata ccggttgccg
    21181 cattgctatc gagtggccag gtaaagtggt agaggccatt ctccggctga ctaacgacgc
    21241 tcctgagtac ctgcccatcg ggtttaatca cgtctaactt gatgggttga ttgggcaacg
    21301 ctttaccgtc tgcatcacgc agcaaaccat tgaggattac cgtttcacct gggcgataaa
    21361 gatcgcgtgg gccaaacatg aaaaactgtt tgctatagcc tggcgcgcca gcaatgttaa
    21421 attctgctaa gtccagcgcc ggaagtttta aatcgagtag cgttgtctga ccgtctttac
    21481 gcgccaacag taatgccgcg tttttatcat tttccagctg cacatgcccc tgagcgtcac
    21541 tggttgcctg agtcagagtc tgccctttct catttaataa agagacttca attccttgct
    21601 gggccgcgcc gttttccaga ctttgggtaa agatatccag acgattgtga taacggtgag
    21661 ctgaaacgcc gatatcactt aacgtaaaca gcgtcgcggg attactgtaa tcgtaacgtc
    21721 cagcctgatt catcacagcc agatacacgc ccgcctgctg aagcggtttg atatcgccca
    21781 gcggcagcaa taatttttca cgggtgttac gcgcaggatt gagatcaaac cgtccggtgt
    21841 agaccagatc cgccatctgc agcagtttgt ctgactgcca gttcgccagc gaattgcggt
    21901 attcccattg gctaatgaat gctggcagag attctggctt aacgcggaag aagttaacat
    21961 cgacattatt aacgttgagc gccattaccg gcagcccttc aacgactttg ccaggcagca
    22021 gcgaaccacg gctggcaaaa ccgacgctgg gttggatgtc gcgggtagtt atagtttttt
    22081 cgtaatcttt actgaaggtt gcgttgttga gtgctttgac ctccttgcca atagtaacga
    22141 tcaaatcacg tttcggttcg aggtggcgta aacgtagctc tttaagatta tctgacagct
    22201 cccaggcacc atccactttg ccgctttttt tatcgacgac atgaataacg cgtgagaaat
    22261 cctgatccgg gtcgagaggg atggagaacg tcagcaccag cgtggctgca ccatcaagtt
    22321 ggacttcaga gagatccagc aatgtcagca ccttaccggc actctgttgg gccagttttt
    22381 gtctctccgg cacggagagt tttgctgaac tcgcgttttc tgaagaggcc gctttagtaa
    22441 cttcagaagg cgcatctttt ttcaccgctg ttggcgcgtt atcgttgttg tcgcaccctg
    22501 ccagcgccag cattagcatg caggcggcta cgcgtaactt tttcattttt catccctgcc
    22561 acaatggccc gttagcaacg tcgaataatt attatgcgtg agaatttacg ttatgtaatt
    22621 cagtatcacg cccatacaat ccgaatttta gtgagaattg tggctcagac agccaaatca
    22681 gcctctttcg cttgtcgctt ttcactaaac atccgacaat ttagcctaac cccggcaaaa
    22741 atggagatgc ctatgtccac gacatggttt gtaggagccg actggctcgc cgaacatatt
    22801 gatgacccgg aaattcagat tatcgatgcc cgcatggcgt cgcctggaca ggaagatcgt
    22861 aacgttgctc aggagtatct gaatggacat attcccggcg cagtgttttt tgatatcgaa
    22921 gcgctttctg atcacacttc cccgcttccg cacatgctgc cgcgcccgga aacgttcgcc
    22981 gtggcgatgc gtgaattagg cgttaaccag gataagcacc tgattgtcta tgacgaaggt
    23041 aatcttttct cagccccacg agcatggtgg atgctgcgca cctttggtgt agagaaagtg
    23101 tcgattctgg ggggtggact tgcaggctgg cagcgcgatg atctgctgtt agaagaaggt
    23161 gcagtagagc tgccggaagg agagtttaac gccgcgttta atcctgaagc cgtggtgaaa
    23221 gtaaccgatg tattattggc aagccatgaa aatacggcgc aaattattga tgcccgcccg
    23281 gctgcacgtt ttaacgcaga agttgatgaa cctcgcccag gtttacgtcg cggacatatt
    23341 cccggagcac tgaatgttcc gtggacggaa ctggtgcgcg aaggcgaact aaaaacgacc
    23401 gatgaactgg atgcgatatt ttttggtcgc ggcgtcagct acgacaaacc aattatcgtc
    23461 agttgcggct ctggtgtaac ggcagccgtg gttttgttag cactcgcgac gctggatgtg
    23521 ccaaacgtga aactgtacga cggcgcatgg agtgaatggg gcgcgcgggc agatttaccg
    23581 gttgagccag tgaaataagt attttacagg caataaaaaa ccgccgaatt tggcggtttt
    23641 ttattgctgg tctggttcgc ggcctttcca gcaggttgta ttaccgtagt aatgcaagcg
    23701 cgtctcagcg gagacaatac tcgccagtaa ctctcttttt gtcaagcaaa agagagttat
    23761 tattgttctg ttagtgtatt atccactgcg gccctttccg ccgtctcgca agcgggcgct
    23821 ggcttaagga aaggatgttc cgcgaccgtt agagcaagcg cgtctcagcg cttgctatcg
    23881 cggcaatatc gccagtggtg ctgtcgtgat gcggtcttcg catggaccgc acaatgaaga
    23941 tacggtgctt ctgtatcgta cttattgttt ctggtgcgct gttttcccaa gtaaataata
    24001 accggagtct ctccggcgac aatttactgg tggttaacaa ccttcagagc agcaagtaag
    24061 cccgaatgcc gccctttggg gcggcatatt ttagattatc cgattctgtt taaagtcacg
    24121 caaaaaacca ccccagcgac gttcatagaa tggcacaata tgttcggtaa taaagtggct
    24181 aattcctttt tccccttttt tcacctgaca aatatcgatt ggttcatcgc caggtaatgt
    24241 atcggtcgct acacttcccg tcgcctgaat aatttcttcg atatcaccat cggcttcaat
    24301 gccaataagt aaattaggct gtgcctcttc gttctcttta attgaacaaa taaaagcacg
    24361 cttcaccgac ttaatggttt taaataaggt ggtgagtgaa tcaatcattt gtgctggcgg
    24421 ctctgcgact tccgataata tcagcgattc accgccttcc aggatttcct ggctgctcag
    24481 cggatttccc tcttcaccaa tcaacaaact gatttcacgc ggcataaatt ctttaccggt
    24541 cggcagtttg gcattaagga agagcgtttc gccaagggtc atctcaaaca atgtgcgaac
    24601 gggcattacg acaaatgcct gttcgtcttc aaccgcctgt tgaagcgctt ctaacgaggt
    24661 gaaaaaagga atgacgctgg tgccgtcttc tttttcccag tgctgtaaat caagcgcact
    24721 atcttcaacc acagcctcgc cctgcgccgc cgtaccaggc acccagacgg tggattccag
    24781 tagagtacgg aaaaaggccg ggcggtgcgc cggttcggtt gctgcttttt ccagcaggtc
    24841 ttcaagttcg ttttttgttt cggacatatg aaccacaatt cattcaacgt tcggggcgca
    24901 aatgcctgat gcgctacgct tatcaggcct acaagggatt cgcaatttgt tgaatttgca
    24961 gaatttgtag gccggataag gcgtttacgc cgcatccggc atattagttt acgccgttaa
    25021 cagattagct atcgtgcgca caccaagtcc cgtagcgccc gcagaccact gttcaaccgg
    25081 cgctttacgg taagtcgccg agcagtcgat atgcagccag ccttgctgat agttctcaac
    25141 aaagtgcgac aggaagcccg ccgccgtgct cgcgcctgcc ggatacgccg cgcttccggt
    25201 attgttcagt tcggcaaagt tagacggcag ctggctgcgg tggaattccg ccagcggcag
    25261 acgccagaat ggttcgttct cttgtgaggc actcgccagc aaacgaccgg caagcgcatc
    25321 gtcaaaactg aacagcgcgt gataatcatt acccagcgca gttttcgccg ccccggtgag
    25381 ggtcgccgca tcaatgatca tttccggttt ctgcgcactg gcatcaatca gaccatcggc
    25441 aagcaccaga cgcccttcgg catcagtgtt catcacttca acttttttac cgttgcgata
    25501 ggtgatgata tcgcccagct tgaacgcatt gccgctaatc aggttatccg cacagcagag
    25561 gaacagcttc acgcgcttgt tcagtccgcg cgtaatggca aatgccagcg ccccggtaac
    25621 cgttgccgcg ccgcccatgt ccgacttcat cgagtccata aacgcagtct gtttgatgct
    25681 gtagccgccg gagtcaaaag tgataccttt acctaccagg cacgcgtaca ccggtgcttc
    25741 tttatcgcca gttgggttgt aatccagcgc cagcaatacc ggagaacgtt ctgaaccgcg
    25801 cccgacggtg tgcagcccca tataaccttg ctcacgcaga tcttcgcctt tggtgatccg
    25861 ataagtcaca cgatcgcccg cgacgttgct gatcagatca acagcacgct gtgccagttg
    25921 cgatggtccc aattcttctg ccggtgcgtt gatggtgtca cgcacccagt cgatgatcat
    25981 caggcggtta tccagttcct ggcgctgggc atcgtccaga tccggccact ccactttacg
    26041 cgtgcctttc ggggctttgt aaccttgcca gaatgcccag cagcgatccg catcccagcc
    26101 ttcaccgctt aactgaacat gcttgatgcc cagaccgtca atcttgcgcg ccgcacgctg
    26161 gatcagcccc agatcgtctg ccccgttcaa atgcagggta atgccgtcat tattaatgct
    26221 gtaagttgct ttttctcccc agcgcgcgtc ggcaggttgg gtagagaggg taatcttcat
    26281 cgcttctgtc attttagtta tccttctttg taaaagggcc gccagttggc agccccagga
    26341 tttttccgct aaatgattcg cgttgcagga aggcggcaag tgagtgaagc cccaggagca
    26401 tagataacta tgtgactggg gtgaacgagc gcagccaacg catctgcggc gtgaagcatg
    26461 acgcggaaat tattcggcct cgtccagcca gactaacaaa atcgcttcga ggattttctc
    26521 gttggatgcc tgcgggtcgt cgtcgaaatc ttccagatcg caaatccact gatgcatatc
    26581 ggtgaatcga accgttttcg gatcaagatc gggatacgca tcgtacagtg cttcgccaat
    26641 ttcgcggcta tcggtccatt taagtcccat actaacctct gttaatgctc acgcgcatgg
    26701 ttgatagtgt aacgcgggat ttcgaccact aaatcttcgt cggtgacgcg cgcctggcag
    26761 cttaaacggc tttccggctc cagtccccag gctttgtcca gcatgtcgtc ttcctgctct
    26821 gagctttccg gcagcgagtc aaaaccttca cgaacgatgc agtggcaggt ggtgcaagca
    26881 caggattttt cacaggcgtg ttcaatctcg ataccgttac gcagagctgc gtcgagaatg
    26941 gtttcaccgc tattagcttc cagaacagcg ccatcagggc agagatcctg atgaggcaaa
    27001 ataacaatct ttggcatatt aaacctcgtc cacggaatgg cctttcagcg cacgacgaac
    27061 cgactggtcc atgcggcgag cggcgaaatc ctgggtttgt ttgtctacgt ttttaatcgc
    27121 ttgttcgatg gcgtcaacgt catcgccctg cgccacttca ctcaggtgag cggcagcatc
    27181 gtcaatgacc tgacgttctg cggcgcttaa cagcgcggca tcagcagcca gcgcgccgtg
    27241 cagactttcc agcacacgcg ccgcttctac tttttgttct gccagcattc gggcttttac
    27301 gtcctgctcg gcatagctca ttgagtcttt gatcatcgaa gcgatttcgc tatcggtcag
    27361 accgtaagac ggtttgacct gaatagacgc ctcaacgccg gtggatttct ccatcgccgt
    27421 cacgctcaaa agaccgtcgg catcgacctg gaacgtcacg cgaatatgcg caccaccagc
    27481 cggtagcgcc ggaataccac gcagcgcaaa acgcgccagt gagcggcagt cctgcaccag
    27541 ttcgcgctca ccctgcatta catggataga catcgccgtc tgaccatctt taaaggtggt
    27601 gaaatcctga gcgcgggcca ccggaatagt ggtattacgc ggaatcactt tctccaccag
    27661 gccgcccatc gtttcgaggc ccagcgacag tgggatcaca tcaagcagca gcatttcgct
    27721 gtctggcttg ttacccacca gaatatccgc ctgaatcgcc gcgccaatag cgacgacttt
    27781 atccgggtcg atggaagtca gcggtggacg accgaaaaat tcgcctaccc gttcacgcac
    27841 cagcggcacg cgagtagaac cgcccaccat caccacttcc agcacttcat cagcttctac
    27901 acccgcgtct ttcagcgcgc gacgacaagc cagtaaggtt cgttttacca gtggcgcgat
    27961 cagttcattg aattgttcac ggctgatttc gccctgccag cccgcaacgt taacggtcac
    28021 ggagtccgca tcgctcagcg cgattttggc tgcaatggcg gcatccagca gttcacgctg
    28081 aacgcggtta tcgctacgat caggaatgcc cgcctgctcg cgaatgtaat ccgccagcag
    28141 atggtcgaaa tcatcgccgc cgagcgcgga atcaccgccg gttgccagca cttcaaacac
    28201 gccgcgactt aagcgcagaa tggaaatatc aaacgtcccg ccaccgaggt cataaacagc
    28261 gatcacgcct tcctgaccgg aatccagccc gtaggcgata gccgcagcgg tcggttcgtt
    28321 aagtaagcgc agaacatgca accccgccag acgcgccgcg tctttggtgc cctgacgctg
    28381 ggcatcgtca aagtacgccg gaacggtgat aactacacca tccagctcgc ctgccagggc
    28441 ttcagttgcc cgcgccgcca gtgctttgag gatgtccgca gaaacgcgca ccgggttcag
    28501 cagccccgcc gccgtttcaa tcatcggcag gccgttttcg ctggcctgga attgataagg
    28561 cagatgcgga tagcgttgct ggatatcagc cagcgagcgt cccatcaggc gtttaacaga
    28621 actaatggtg ttggcggtat caagcgctgc gttagtacgc gcgtcataac ccaccgaatg
    28681 cccttgctgt tgatagtgaa caacagatgg cagcaggtga cggccttcat ggtcggctaa
    28741 cgtttcggcc tgaccgctgc gcactgtcgc caccagcgag ttggttgtgc ccaggtcaat
    28801 accagccgcc agacgacgct gatgcggcgc agcactcaaa ccaggttcac taatttgtaa
    28861 taaggccatg tttagcttcc agaaattaaa aatcgagcag tttttcttcg agttgttcgg
    28921 cactgctccg cagtttatcg agaaaacgca gcttacgcac ggtatccgcc gccgcgtccc
    28981 acgcctcgtt gtctaactgt tcaaccatca actgatggcg ggtatcaaac atctttttca
    29041 cacgtttgat aaagctttcc agccgcgctt catctttcgc ctgttcgatc tcgtccagct
    29101 cttcgcgcag ctccaactgt tccatcagga acgcggtgtc gcgcacagta tgctgctcgc
    29161 tggcgagatc aaaaccgtgt aaagaaagca aatattccgc gcgcattaac ggatgacgca
    29221 gcgtttgcca ggcctggtta atggttgcag attgctgtac ggcggcgagt tgttccgcct
    29281 ggcttccgct ggcgaattta tcaggatgat actgacgttg tagatcctga aaacgcaggc
    29341 tcagcgcctg ggtatcgagt tgataacggg caggcaagcc aaagagggtg aagtaatcca
    29401 taacattctc agggctgcga tccgccgcga taacggcgaa tcaggtaaaa caaaccccac
    29461 gcgcaggcga ccacggtggg gttatcggta tgcgcatcaa acgtggaagc tttcgccgca
    29521 accacactca tctttgacgt tcgggttggt gaatttaaac ccttcgttca ggccttcttt
    29581 tacgaagtcc agctgcgtac cgtccagaaa ttgcaggctt ttgccatcga ccacgacttt
    29641 cacgcctttg tcttcaaaca cgatgtcttc cggcgtcggt tcgtcaacaa attccagtac
    29701 ataagccata cctgaacatc cggaggttct cacgcccaga cgcaggccaa accctttgcc
    29761 gcggttagcc agaaaggtat ttactcgcgc tgctgcactg tcgctcagtg taatcgacat
    29821 aaccaaacct caactcttat tttgcttcac gtttgctttt atagtccgca atggcggctt
    29881 tgatcgcgtc ttctgccaga atagaacagt gaattttcac cggcggcagt tcaagttctt
    29941 cagcaatatc ggtgtttttg atcgcctgcg cttcgtcgag agacttccct ttcacccatt
    30001 cggtgaccag ggagctggaa gcgatagcag aaccgcagcc gtaagtttta aaacgcgcgt
    30061 cttcaatgat accttcatcg ttgactttaa tctgcaactt catcacgtcg ccacaggccg
    30121 gtgcccccac catgccgctg ccgacgttct cgtcgttgtt gtcaaaggaa cccacgttac
    30181 gcggattctc gtaatggtcg ataacttttt cgctgtaagc cattataaat tctcctgatt
    30241 ccgataccga ttaatgatga gcccattcga tgctgttcag atccacgccc tgcttgtaca
    30301 tttcccacag cggagaaagg tcacgcagac gaccgatgga tttacgaact aactcgatgg
    30361 tgtagtcgat ctcttcttca gtagtaaaac gacctaaaga gaaacggata gagctatgtg
    30421 ccagctcgtc gttcagcccc agcgcgcgca gcacgtagga cggttcgagg cttgctgacg
    30481 tacaggcgga acctgaagaa actgcgaggt ctttcagcgc cataatcagc gactcacctt
    30541 caacgtagtt gaagctgacg ttgagaatgt tcggcgcacc gtgttccagg tcaccgttca
    30601 ggtaaacttc ttcgatatct ttgatgccgt tccacagacg gttacgcagg ccgcgcagac
    30661 gttccatctc ggtcgccatc tcttcttttg cgatgcgata ggcctcgccc attccgacga
    30721 tctggtgaac aggcagagtg ccggaacgca taccgcgctc gtgaccgccg ccgtgcattt
    30781 gcgcttcgat gcgtacgcgc ggtttacgac gtacatacag cgcaccgata cctttcgggc
    30841 catagatttt gtgaccggag aaagacatca ggtcaacttt caactggctc aggtcgatag
    30901 gcagtttgcc cacgctctgg gttgcatcaa cgtgatagat aatgccacga gcacggcaca
    30961 tttcgccgat agccgcgata tcctgcacca cgccgatttc gttatttacg tgcatgatgg
    31021 aaacaaggat ggtgtcgtca cgcatcgctg cttcaagttc tttcaggtcg ataatgccgt
    31081 tacgctgcgg tgccaggtag gtgacttcaa aaccttcgcg ctccagctga cggcaggtat
    31141 ccagtaccgc tttgtgttcg gttttgctgg tgatgatgtg cttgcctttt ttctgataaa
    31201 agttggctgc acctttgatc gccaggttgt cagattcggt tgcaccagag gtaaagacga
    31261 tttcacgcgg atcagcgccg accagatcgg caatctgatt acgggcgata tctaccgctt
    31321 cttcagcctg ccagccgaaa cggtgagaac gagaggccgg gttaccaaag gttccgtcca
    31381 tcgtcataaa ctgcatcatt ttctcggcaa cacgcgggtc caccggcgtg gttgcggagt
    31441 agtcgagata aatcggtaat ttcattgctc tataaactcc gtacatcact caatgcaagg
    31501 aaccaggcta ccggctggat gtacgaccgt gtttacggag cgcgatacgc cccggcctga
    31561 ttctgaattc tttttattaa gcgcgtaact taacgtcgat cgcgtcttgt atgcgggtgc
    31621 gtggcgcgtc gtgagtatgc tgacgaccag acacatccag cacttcctgg ttattaacca
    31681 gttcgcctaa agtaatgttg ttgagaaaac cggtgagacg gtcgctcaaa tcacgccaca
    31741 gcgcgtgggt caggcattta tcgccgccct ggcagccgcc tttaccctga caacgggtgg
    31801 catctacaga ttcgtcaacg gcgctaatca cttcgccaac ggcgatgctg ctggcatctt
    31861 tgcctaacag ataaccaccg cctggtccac gtacgctgga aaccagacca tttttacgca
    31921 gacgggaaaa cagttgttcc agataagaaa gggaaattcc ctgacgttcg gaaatatcag
    31981 ccaacggtac cgggcccgct tcagagttga gcgcaacgtc aagcattgcg gtcacggcat
    32041 agcgcccttt agatgtcagt ctcatgtctt acttcacctc aaactcgccc ctgcccgggg
    32101 ttttttattg taaaagtggg ggtattgcat agcagggtca agtctgacat tcccgagtaa
    32161 attggtcaac tatttacttg actgatttag tcgggtattt aaccttcagt gccatttttt
    32221 tatcgtggcg tttgtacctg ttgtccttcc agaccatggg cgggttgagg gcaattattc
    32281 gcattagcgc tggcctcttc ccctcaccct aaccctctcc ccgaaggggc gaggggaccg
    32341 tccgtgcatt tatctggctt agccctcagg ccttattacc tttattctgc tgctcaatag
    32401 aagccagaat cccgcgcagg atattcaatt cctggctttc cgggcgcgca cgggtaaaca
    32461 gacggcgcaa tttattcatc acctgccccg gatggttttc acggataaaa ccggttgcca
    32521 gcagcgtttg ttccagatga ccataaaaac gctccagatc atcgaccagc ggatacggcg
    32581 tctcttcatg ctcgacctgc tcgccgtttt cctgagttgc cagccaggcc atgcgcactt
    32641 cataggcaat aacctgaacc gccatcgcca ggttcagcga gctgtattcc gggttagccg
    32701 caatcgcgac atgataatgg catttctgca actcttcatt ggtcaagccg acgcgctcac
    32761 gaccaaacac cagcgccacc ggggtatttg ccgcttcagc gacgcttttc agaccgcatt
    32821 cgcgcgggtc gagcatcggc cacggcagcg tgcgggagcg tgcgctggtg cccaccacca
    32881 ggctacaacc ggctaacgct tcgtcgagcg tatcgacgat gtgagcatta ccaatcacat
    32941 cgctggcccc tgctgccagg gcaatcgcct gggagtcggg tttcaccagt ggattaacca
    33001 gccacagatt ggttaatccc attgttttca tggcacgggc aacagaaccc atattgccgg
    33061 tgtgtgacgt ctccaccagc acaattcgaa tattttgcag cattgtcttt cttcatctaa
    33121 agattattca cgcatcttat cataaaacga agacagatgc cgatctcgct gctatactct
    33181 gcgccgtttt cccgttcttt aacatccagt gagagagacc gatgcatccg atgctgaaca
    33241 tcgccgtgcg cgcagcgcgc aaggcgggta atttaattgc caaaaactat gaaaccccgg
    33301 acgctgtaga agcgagccag aaaggcagta acgatttcgt gaccaacgta gataaagctg
    33361 ccgaagcggt gattatcgac acgattcgta aatcttaccc acagcacacc atcatcaccg
    33421 aagaaagcgg tgaacttgaa ggtactgatc aggatgttca atgggttatc gatccactgg
    33481 atggtactac caactttatc aaacgtctgc cgcacttcgc ggtatctatc gccgttcgta
    33541 tcaaaggccg caccgaagtt gctgtggtat acgatcctat gcgtaacgaa ctgttcaccg
    33601 ccactcgcgg tcagggcgca cagctgaacg gctaccgtct gcgcggtagc accgctcgcg
    33661 atctcgacgg taccattctg gcgaccggct tcccgttcaa agcaaaacag tacgccacta
    33721 cctacatcaa catcgtcggc aaactgttca acgaatgtgc agacttccgt cgtaccggtt
    33781 ctgcggcgct ggatctggct tacgtcgctg cgggtcgtgt tgacggtttc tttgaaatcg
    33841 gtctgcgtcc gtgggatttc gcggcaggcg agctgctggt tcgtgaagcg ggcggcatcg
    33901 tcagcgactt caccggtggt cataactata tgctgaccgg taacatcgtt gctggtaacc
    33961 cacgtgttgt gaaagccatg ctggcgaaca tgcgtgacga gttaagcgac gctctgaagc
    34021 gttaatgact caggcgggtg atgtcactca cccgccctcg cctttcaggc gctattctga
    34081 aatacttcct caccgcttta ctttctttcg tcactctccc accatctttc cccgattaat
    34141 ggatgataaa gaagtcgcaa ggaaaggtta tggcactgcc agtgaacaaa cgcgttccca
    34201 aaattctgtt tattctcttt gttgttgcct tctgcgttta tttagtgccg cgcgttgcca
    34261 tcaacttctt ctattatcct gacgataaaa tttacggtcc ctacccctgg tcggcggaat
    34321 ccgtcgaatt tacggctaag gacggtactc gtctgcaagg ctggtttatt ccttcttcga
    34381 cgggccctgc tgacaacgcc atcgcaacca tcattcatgc tcacggcaat gccggaaata
    34441 tgtccgccca ctggccgctg gtcagttggt tacctgagcg taatttcaac gtttttatgt
    34501 ttgattatcg cgggtttggt aaatcaaaag gcacgccgtc ccaagccgga ttgctggacg
    34561 atacgcaaag tgccatcaat gtggtgcgcc atcgcagtga tgtaaaccca caacggctgg
    34621 tgctgttcgg gcagagtatt ggcggggcga atattctgga tgttattggt cggggtgatc
    34681 gtgaaggcat acgtgcggtg atcctcgact ccacatttgc ctcttatgca accatcgcca
    34741 accagatgat ccccggcagt ggctacttac ttgatgagag ttacagcggc gaaaattata
    34801 tcgccagcgt cagcccgatc ccgcttttac tcattcacgg taaagctgat cacgttatcc
    34861 catggcagca cagcgaaaag ttgtatagcc tggcaaaaga gccaaaacgg ttgatcctaa
    34921 tcccggatgg cgaacacatt gatgcttttt ccgatcgtca cggcgatgtt tatcgcgaac
    34981 agatggtgga ctttatcctt agcgcgttga atccacagaa ctaacccatg atcgctagca
    35041 cgataatcat tcacaaaacc accttaagac atgctaatcc actggtcaga acagtttaag
    35101 atgagaaaaa ttctgtgacg cttgccaaca tttctgatga ttagcattcc cttcgccatt
    35161 tccctgagca aactttagct attcttatca attatgctta tgggagatct acagatgatg
    35221 cctacgcttg ctccaccatc tgtcctttcg gctccccagc gccgctgcca gatcttgctg
    35281 acgctctttc agccggggtt gaccgccacc acggcaactt tcagcgagct taatggtgtg
    35341 gatgatgata ttgccagtct tgatatcagc gcaacaggac aggagatcct gcgctatcat
    35401 caactcacac tgacggctgg ttatgacggt agctaccggg ttgaaggtac agtgcttaac
    35461 caacgtttgt gtttatttca ctggctacga cgtggtttcc gtctgtgtcc atcatttatt
    35521 accagccatt tcacccccgc cctgaagagt gaactgaagc ggcgcggaat tgcgcgtaac
    35581 ttttacgacg ataccaatct acaagcgtta gtgaatctct gctcccgacg actgcaaaaa
    35641 cgctttgaaa cgcgcgatat tcatttcctg tgtctgtatc tgcaatattg tttgctgcaa
    35701 caccacgctg gaattacgcc tcagtttaat ccgctccaac gtcgctgggc ggaatcctgc
    35761 cttgaatttc aggtagcgca ggaaattgga cgccactggc agcgtcgggc gctccagcct
    35821 gtaccgcctg atgagccact gtttatggca ttactttttt ccatgttgcg ggttcccgat
    35881 ccattgcgtg atgcgcatca gcgagacaga caattgcgtc agtctatcaa acgtctggta
    35941 aaccattttc gtgagctggg aaatgtccgt ttttatgatg aacaggggtt atgcgatcag
    36001 ctttataccc acctcgccca ggcgttaaat cgcagttttt ttgccatcgg tattgataat
    36061 accctgccgg aagagttcgc cagactgtac ccacgcctgg tgcgcaccac ccgcgcggcg
    36121 ctggccggat ttgaaagtga atacggcgtc catctttctg atgaggaaag tggtctggtc
    36181 gcggtgattt tcggtgcctg gctaatgcag gaaaacgatc tgcatgaaaa acagattatt
    36241 ctactgaccg ggaatgatag cgagcgagaa gcgcagattg agcagcagtt acgcgaacta
    36301 acgttactgc cgctcaacat taagcatatg tcggtaaagg catttttgca gacaggcgct
    36361 ccgcgtggcg cggcactgat tattgcgcct tataccatgc cattaccgct cttttcacca
    36421 ccgctgatct atacggacct gacgctgaca acacatcaac aggagcagat ccgcaaaatg
    36481 ctcgaatcag catgagggaa caactttcgg gcgcaaaaac attgccggca gcgccaccag
    36541 cgccattacc cagaacacgc cgtggcccag atattgatac aggaaaccgg cgaaaacggt
    36601 catgatagcg atactgccgc ccatcgcgac ggcagagtaa accgcctgta aacggatgac
    36661 ttcgctaccc tggcgagcag caatataacg catggcggcc aggtggcaga ccgtgaaggt
    36721 gccgcaatgc agaatttgca ccactatcaa ccacggcaac gccgtagttg ctcccataat
    36781 gccccagcgc actacgccgc aaatcgccga gatcaacagc atatcgcgtg cactacaacg
    36841 gcggaaaagt ttattactca gcgcaaagat aatgacttcc gccaccacgc ccagcgacca
    36901 caaatacccc accgccgagg ccgagtagcc agctgcctgc cagtaaatgg cgctaaaacc
    36961 gtaataggcc gcatgtgccc cctgcaataa acaaacgcag gccagaaagc gccagttctg
    37021 gcgaaccagc gccaaccacg ctgaccaacc ggtgctctcc tgctggcggc ttgccccttg
    37081 tggctgaatc gtcggacgga tgagaaagcc gagcagcatg gatgccacgc ccaacgtcaa
    37141 cagcgcgagg atcacccgat aatcaaacat agtgaccagt ttgcccgtca gcgccgagcc
    37201 aatgacaaac gccaccgagc cccacagtcg cactttgccg taatcaagcg ggaactgctt
    37261 ttgccacgta ttcgccagtg catcggtcaa cggtaccagc ggtgagaaaa agaggttaaa
    37321 gccaatcatc accagcatca gccacgctac gtgcgccccc gcccagaagg cgacagcaaa
    37381 gagaagtgtc agcagtgcca gcacgcgcaa ggcggaaatc aggcgggaag gatcgctgac
    37441 gcggggcgcg atgagcaaac tcccgagaaa acgggcaacc agacctgccc ccaataacag
    37501 gccgatggtt tctggcgtta aaccaatccc tttaagccag acgctccaga aaggtagaaa
    37561 aatgccgtaa ctaaaaaagt atgtgaaata gccgagcgcc aaccagcgcg tggattgcaa
    37621 aaccatgagt ccctcccgtc aggaggcgtt agtctggggt taatcgaatg ttggtgcaag
    37681 ttgaaaaata agaatattcg agcctgcttc ccgatgtctg taacgccagt tcacaagcac
    37741 taaaaaactg agatccgcga cgcatgttgc aaaaataatg cgtaattatg ccgttacgct
    37801 tgccaaacgt tcctgcacaa tggcgatgaa atcgcgtaat gccggcttca tctccccttt
    37861 cttccacgcc atcagtaaag caatggaagg cacatttccg gcaatgggac gaaaaacaac
    37921 ctgtccggta ttaaaattat tcatataacc gggtatcaaa gtgacgccca gccccatgcc
    37981 caccagattc atggtcacca gaatattcgt tgccacctgg acgatatttg gctggctgtt
    38041 ttcttgcgca aaccacgctt taacgatcgg cgcaagcgaa ccggaatacg ccggatcggt
    38101 actgacgaaa ttcacgccat ccagttgcgc ggcggtgatc tctttttcat gtgctaaagg
    38161 gtgatcaacc ggtaacacaa ccactaatgg ttcgtcaaaa agctccagat aatcaatctc
    38221 cgggctataa acaggatggc gcattaagcc gacgtcgagt tcaccacggc gaattttttc
    38281 ctcctgttgc gtggtgatta aactcaccag ctcaatcaag gtgtctggct gtctgagacg
    38341 aaacatcggt aatacttttg gcagtaaatt cacttccgcc gatggcacaa agccaatggt
    38401 taattgtctg tcttcctgaa caattttccg cgcccgtaat ttggcatttt ccgcttgttc
    38461 gaggattgcc agcgcgtcct ggagaaaaca ttctcccgcc gctgtcaacg cgactttgcg
    38521 cttatccctc accagtaacg gaacaccgac acagttttca agatcgcgga tctggctgct
    38581 taacgaaggc tgtgaggtat gcagtttttc cgccgcacgg gtaaagttca gtgcctgcgc
    38641 cactgcgacg aaatagcgta aatgccgtag ttccatcacc ttccccttgt tatcgaaaaa
    38701 acgtctcagc tggtagaaat taactatttc acatattagc aaccaaccag caacatcctt
    38761 atggcacaaa aatagaaggt caatacatct tatctttcag gattaaaaaa tatgaccaca
    38821 ccctcagatt tgaacattta ccaactgatt gacacccaaa atggtcgggt cactccgcgt
    38881 atttataccg acccggatat ttaccaactg gagcttgagc gtattttcgg tcgttgctgg
    38941 ctatttctcg cccacgaaag ccagatccca aaacccggtg atttctttaa cacctacatg
    39001 ggagaagatg cggttgtcgt agtgcgtcag aaagacggca gcatcaaggc gtttctcaac
    39061 caatgccgcc accgggccat gcgtgtgagt tatgcagatt gcggcaacac tcgcgccttt
    39121 acctgcccgt atcacggctg gtcttatggc attaacggcg agttgatcga tgtaccgctg
    39181 gaacctcgcg cctacccaca agggttgtgt aaatcccact ggggactaaa cgaagttcct
    39241 tgtgtggaga gttataaagg gctgattttt ggcaactggg ataccagcgc accgggcctg
    39301 cgtgattacc tgggtgacat tgcctggtat ctggatggca tgctggatcg tcgcgaaggc
    39361 ggcaccgaaa ttgtcggcgg cgtacaaaag tgggtgatca actgtaactg gaaattcccg
    39421 gcagagcagt tcgccagtga ccagtatcat gctctgttca gccatgcttc tgccgttcag
    39481 gtattagggg cgaaagatga tggcagcgat aagcgcctcg gtgatggaca aaccgcccgc
    39541 ccggtgtggg aaaccgccaa agatgcgctg caatttggtc aggacggtca cggtagcggt
    39601 ttcttcttta ctgaaaaacc ggatgctaat gtctgggtcg atggcgcagt ttcaagctat
    39661 taccgcgaaa cctatgccga agcagaacaa cgtttaggtg aagttcgcgc cctgcgcctg
    39721 gcgggtcata acaatatttt ccccacgctt tcatggctaa acggcactgc cacactccgc
    39781 gtctggcatc cgcgcggccc tgatcaagtt gaagtgtggg cgttctgtat tactgacaaa
    39841 gccgcctccg atgaagttaa agccgctttt gaaaacagcg ccactcgtgc ttttggtcct
    39901 gctggttttc tcgagcagga tgactcggag aactggtgtg aaatccagaa attgcttaaa
    39961 ggccaccgcg cccgcaacag caaactgtgt ctggaaatgg ggcttggtca ggaaaagcgc
    40021 cgcgacgacg gcattcctgg cattactaac tatatctttt cagaaactgc cgctcgtgga
    40081 atgtaccaac gctgggccga tcttctgagt agcgaaagct ggcaggaagt gctcgataaa
    40141 accgccgctt accagcagga ggtgatgaaa tgagtgcgca agtttcacta gagttacatc
    40201 accgcattag ccagtttctc tttcacgaag ccagcttact ggacgactgg aaatttcgtg
    40261 actggctggc gcagctcgac gaagagattc gttacaccat gcgcaccaca gttaacgcgc
    40321 aaacacgcga ccgccgcaaa ggcgtccagc caccgacaac ctggattttt aatgacacca
    40381 aagaccagct ggagcggcga atcgcccgtc tggaaacggg catggcctgg gcagaagagc
    40441 cgccgtcacg cacccgtcac ttaatcagca actgccagat aagcgaaacc gacatcccaa
    40501 acgtatttgc tgtgcgggta aattatctgc tttatcgggc acaaaaagag cgcgatgaaa
    40561 cattctatgt tggaacgcgt ttcgacaaag ttcgccgtct ggaagatgac aactggcgct
    40621 tgctggaacg ggatatcgtc ctggatcaag cggtaatcac ttcccataac ctgagtgtac
    40681 tgttctgatg aatcgaattt atgcgtgtcc cgttgcggat gtgccggagg gtgaggctct
    40741 ccggatcgat acctcgcccg tcatcgccct gttcaacgtt ggcggcgagt tttatgccat
    40801 taacgatcgt tgcagccatg gtaatgcgtc gatgtcagaa gggtatctgg aagatgacgc
    40861 cacggtggag tgcccgctac acgccgccag tttttgcctg aaaacgggga aagcgttatg
    40921 cctgcccgcc accgatccgc tcaccactta tccagtacac gttgaaggtg gtgacatttt
    40981 catcgactta ccggaggcgc agccatgagc gatctgcata acgagtccat ttttattacc
    41041 ggcggcggat cgggattagg gctggcgctg gtcgagcgat ttatcgaaga aggcgcgcag
    41101 gttgccacgc tggaactgtc ggcggcaaaa gtcgccagtc tgcgtcagcg atttggcgaa
    41161 catattctgg cggtggaagg taacgtgacc tgttatgccg attatcaacg cgcggtcgat
    41221 cagatcctga ctcgttccgg caagctggat tgttttatcg gcaatgcagg catctgggat
    41281 cacaatgcct cactggttaa tactcccgca gagacgctcg aaaccggctt ccacgagctg
    41341 tttaacgtca acgtactcgg ttacctgctg ggcgcaaaag cctgcgctcc ggcgttaatc
    41401 gccagtgaag gcagcatgat tttcacactg tcaaatgccg cctggtatcc tggcggcggt
    41461 ggcccgctgt acaccgccag taaacatgcc gcaaccggac ttattcgcca actggcttat
    41521 gaactggcac cgaaagtgcg ggtgaatggc gtcggcccgt gtggtatggc cagcgacctg
    41581 cgcggcccac aggcgctcgg gcaaagtgaa acctcgataa tgcagtctct gacgccggag
    41641 aaaattgccg ccattttacc gctgcaattt ttcccgcaac cggcggattt tacgggtccg
    41701 tatgtgatgt tggcatcgcg gcgcaataat cgcgcattaa gcggtgtgat gatcaacgct
    41761 gatgcgggtt tagcgattcg cggcattcgc cacgtagcgg ctgggctgga tctttaagga
    41821 agcacgatga aagaaaaaac catcattatt gtcggtggcg ggcaagcggc ggcaatggct
    41881 gcggcctcgc tacgccagca agggttcacc ggtgagctgc atctgttttc cgatgagcga
    41941 catcttcctt atgaacgacc tccactctcg aaatccatgt tgctggaaga ttccccgcag
    42001 ttacagcagg tgttacccgc taactggtgg caggaaaaca atgttcatct gcattccggt
    42061 gtaaccatca aaacgctggg tcgcgacaca cgagagttag tgttaaccaa cggcgaaagc
    42121 tggcactggg atcagctttt tatagcaacc ggcgcggcag ctcgaccgct gccgttgctt
    42181 gatgcactgg gagaacgctg ctttacccta cgccatgccg gtgatgccgc cagactgcga
    42241 gaagttctgc agcccgaacg gtcagtcgtg attgtcggtg ccggaactat tggtctggaa
    42301 ctggctgcca gcgccacgca gcgcagatgt aaggtgacag tgattgaact ggcggcaacc
    42361 gtcatgggcc gtaatgcacc accgcccgtg caacgctatc ttttacagcg ccaccagcag
    42421 gctggtgtgc gcattctgct caataatgcc attgaacatg tggtcgatgg tgaaaaagta
    42481 gaactgacgc tgcaaagtgg ggaaacgctt caggctgatg tggtgattta cggtattggt
    42541 atcagcgcca acgagcaact ggctcgcgag gccaaccttg atactgccaa tggcattgtc
    42601 attgatgagg cttgccgcac ctgcgatccc gcgatctttg ccggtggcga tgtggcaatc
    42661 actcgtcttg ataatggtgc actacaccgc tgcgaaagct gggaaaacgc caataaccag
    42721 gcgcaaattg ccgctgccgc aatgttaggg ctaccgctac cgctactgcc gccgccgtgg
    42781 ttctggagcg atcagtacag tgataactta cagtttattg gcgatatgcg tggcgatgac
    42841 tggctttgtc gtggcaaccc ggaaactcag aaggcgattt ggtttaatct gcaaaacggc
    42901 gtgcttatcg gtgcggtcac gctgaatcag gggcgtgaga ttcgcccaat tcgcaaatgg
    42961 atccagagcg gcaaaacgtt tgatgcgaaa ctgctgatag atgagaacat cgcgcttaaa
    43021 tcactgtaac caggataatt agcgaatatc tcaatgcctg gggcgtggcg aggtgcaaga
    43081 gtgtgtatta cgtttaaatc acattatctt gcaaagggat tggttatgaa cacactacgt
    43141 tattttgatt ttggagctgc ccgccccgtt ttgttattaa ttgcccgtat cgccgtggtc
    43201 ttaattttca ttatttttgg ttttcccaaa atgatgggct ttgacggtac ggtccaatat
    43261 atggcctcgt tgggcgcgcc gatgccgatg ctggcagcga ttattgcggt agttatggaa
    43321 gtgcccgccg cgatattaat cgtgcttggc tttttcaccc gtccgctggc ggtgctgttt
    43381 attttctaca cgctgggtac ggcggtgatt ggtcaccatt actgggatat gaccggcgat
    43441 gcggttgggc caaatatgat taatttctgg aagaatgtca gtatcgctgg cgcattcctg
    43501 ctgttggcaa ttaccggacc gggggcgatt tctctcgatc ggcgttagga aagatgccgg
    43561 atgcggcatg aacgccttat ccggcatttt ataaattaca gcaacgcaac ccgcaacgac
    43621 atctctgaag ttgttgattc acctggtgca agcgcaagaa gatcaccccc ttccgggcga
    43681 tgatgatcat ccggcgcatg gctcatcggt tccagacaga agaaatcaaa cgcatatcct
    43741 ttatcaaacg cagggtcgga aacaaagatg aaataacacg gtgcaggtgg cgtcgtttcc
    43801 atgatgatgg cataaccttc ctgcggctgc tcgatgcggg cctgaccatt ccatccggca
    43861 aaaccattgt tcacccactg gcgcggcaac ggcgcgggct ggttaaaatc cagttcctgc
    43921 ggtagctgct cgcaaaactc acccgccagc cactgctccc gctccagcca gtaaccgctc
    43981 gcctgtgcct gaatccgcgt ttgcggcgac aacggaaaat aaggatgcca gccggtacca
    44041 aatggcagcg tctctgcccc ctgattggtg acagagagcg tcaccgtcag cgtatccgcc
    44101 gttaaatgaa acgcctgact tacccgatag tgatagacac cgctgcgatg ttcatacacc
    44161 agacacaaac tatcatcgct gtgcgagaca cattgccact cgcccagcca gccatcgccg
    44221 tgtagatagt gcgcatccca ctcaacattc ggttgcagtt gatactcacg cccctgccag
    44281 acaaaacgat tgccgctcac ccggtttgca aatggcacca gcggaaaaca tgaggcatcg
    44341 gttgccacac cgcttttttt accaggacgt aagagcggcg tcgtatcgcg ccagaagcct
    44401 tcgattacgc cgccctggtc agaaacgtcc agctttagcg acccgtggga taaggtatag
    44461 atggtcatcc gctaactcct taatccggga agttaatcac aactttcccg catttgccgc
    44521 tcgccatcag cgcataggca tcacctgcct gttccagcga gaagcgatgg gtaatggcgt
    44581 tacgcggcca cagcttccag tccgtcagat catgggcgca tttttccata tggaacagac
    44641 tggtcaccca ggagccgata atccgccgtt gatggtgcat cagatcggcg ctgacctcga
    44701 attccacttt tccggtttca ccaatgtaaa ccacccgtcc ccagtcagcg gtggattgca
    44761 gtgccagcaa gcgacctgcg gcattaccgg aacaatcgag cgcaacatcc gcgccaccgt
    44821 gggtgagttc ggcgataatc tgcggcagac cttcggtggt tgctaaatag ccgtgatcca
    44881 tcacccctaa ctgttttgcc atcgccagac gttccggcag catatcaacg ccgatgatcc
    44941 gttttgcacc gcgacctttc gccagcatca tcgccatcat gccgaccggc cccagaccga
    45001 ccaccagcac gttatcgctg ccggaaactt cgccgcgcaa aatcccttca taggccgtgc
    45061 caacgccgca actgataaac gcaccatctt cgtagctcag cgcatccggc aggaggatca
    45121 gatctttttc ttccgccagc agatattcgg catgaccgcc gtcacgctgc cagccgtaag
    45181 ccgcttttcc tttgccagta caagagatag gaaagccgcg gcggcagttc gggcaaaaac
    45241 cacagccaga aatgtgatac accagcacgc ggtcgccctc tttaaaatgg cggcagcctt
    45301 gccccatcgc cacaatctgc ccgcacggtt catgaccgtt gataaatcct tggtataacg
    45361 gtttgtcggg tgccgctgcc gtagcgcggt gctggtggta gatatagtgg acatcgcttc
    45421 cgcaaattcc ggaagatttc attttgatca gtacctggtt aatccctggt gtcggcaccg
    45481 caacttcccg tagatcgacg gtcgaatttc ctggtaaata agctgccagc atcgttttca
    45541 taaatccctc attaacaata cgattaattt tcatccctgc cgcacccgcg ccaggggcgt
    45601 taaggtttag cgtttcgctt tactgcttcg ctgggtcagc aagatattcg ccagcaccgc
    45661 caccacgatg atgacgccgc gtaccacctg ctggaaaaag gagttaatac cgagcagcac
    45721 cagaccgtta ccgattagcg taatcaccag cacaccaagc aatgtaccga acagggaacc
    45781 gcgaccgccg gaaagtgccg taccgccgac cacgaccgcg gcgatgacgt caaactccag
    45841 accgtttgcg gcacctgcgt taccagaacc gaggcgcgcc gccaacaaaa tgccggtcac
    45901 cgccgctaat aatcccgaaa gggtaaagat aagaatgcgc acccgacgaa cgttgatgcc
    45961 gcacaactgc gccgccgtag cattaccgcc aacggcaaaa accgagcgcc cgaaggcggt
    46021 tttgcggctg atgaacacaa acagcgcaaa caacacaatc atgatcagcg cggataccgg
    46081 cacaccgaga aattgtccgc ccagccagtc cagcacctcg ttttcgtcaa tcggcactgg
    46141 cagtgcgttc gtcataaaca gccccattcc gcgcagggcg ctccacagcc ccagtgtggc
    46201 aacgaaactt ggtacgttaa acacgccgcg cagcaccccg gccagcgttc ccatcagcgc
    46261 gcctaacagc aacaccagca gacacgccac cgccagcgga acttcaaatt gcagcaaaaa
    46321 tgccaggcac accgagacaa aagccaccat cggcccaacg ctgacatcaa tttcaccgga
    46381 gataataatc agcgtcatcg cccaggcggc aatcccaatg gtggcggcat cgcgcagcac
    46441 gttcatctgg ttattcaatg agataaagcc aggcgcgttc agggagaaga ccagataaag
    46501 aatggcgatt accaccagaa aaccgatctc attaatatgg cgactgacaa attgtttgag
    46561 cgagacgctc ttgccctgcg gcaatgataa tgacgaagca gacatcttca ggttcctcgg
    46621 taaaattcat cagtgcacag acagaatggc ggacatcagc tcatccacat tgaccggcga
    46681 gtgaaactcc tgcgagaacg taccgtgctg taacaacagg atgcggtcac acaccagcgg
    46741 caactcctcc acttcactgg agataaacac cacgcttttt ccttcggcag ccagctcacg
    46801 gacaatacgg taaatctgct gtttggcttc gatatcgacg ccgcgcgttg gctcgtcgag
    46861 caacaaaatc tggctggcgg catagaccca acgaccgatc accacttttt gctgattgcc
    46921 gccagaaagc gtgccgatgg gcgtttcgct actggcggcc ttgaccgtca tccgctgcat
    46981 cacctcttcg gtcaggcggc ggatggtgga ccattgcagt acgccgttgg cgctgatttt
    47041 ttgccgattg gtcagcactg tattttcgtc aacgcccaac cagggaatga tccccgcttc
    47101 tttgcggttt tctggcgtat agccaatgcc gcgtttcagc atgtcgccgt aatcggggcg
    47161 cgtgattttc tcgccgttga taacaatttc gccctgttca tactcctcca gcccaacaat
    47221 cgccttcagc aattcactgc gccctgcccc cagcaggcca gcaatgccaa gcacttcgcc
    47281 gcgacgtaac gtaaagctga tatcctccag cttgggctta tggcgtaatg cacggacttc
    47341 cagcacggcc tgatccacaa tttcctgagg ggctaccggc gcaatatcaa cgtgatcacg
    47401 ccctagcatt agcgacacaa tatgatgcgt ggaggtgttt tcgagcatca catcgcccgc
    47461 cacctgacca tcgcgcataa cggtggcaca ggaggcaatg cggcggattt cttccatccg
    47521 gtggctgaca taaatcaccg ccacgcccag tgccgacatc tttttcaccg cactgatcac
    47581 cagttcaact tctgcactgg caagcgagct ggtgggttca tcaagaatga ccacgcgcgg
    47641 ctcgcccttc atcacccgtg caatttccac cagttgcttt tgcgccgggc ttagcgttga
    47701 aacaagttgt tcaggactaa cgtcaacgcc cagcgcctgt aagcaacgtt gggcatcctg
    47761 cgccatttgc aggtaatcaa tcatgccgtt gcggcgaggc cattgaccaa ggcagaggtt
    47821 ttctgccacc gtcagccctt ccaccagact taattcctga taaaccgcac gaacccccag
    47881 ttcagcggca cggcgagtca acgtagcttc gtcaccttcc agtcgcgtct cgccaatcca
    47941 gatatcaccg ctatccgggc gttcgctgcc ggtaagcatt cgaatgagag tcgatttgcc
    48001 cgcgccgttt ttacctaaca gcgcacgaac ttcgccttta ttgagcgtga agttaacgtt
    48061 atccagcgca acgacgccgg ggtaacgctt atttcctgcc accacttttg ctaccgggac
    48121 tgcctctgtt gccgtgaaca tagggacctc tgcgaatcag cgattagggc agaccatcaa
    48181 cgtgcgttgc cagccactgt ttaccgtctt ccgttttggt atagagatcg attggcacct
    48241 gaatcacttt ttcaccgtcg gcttgtttat tgataacctt caatgtttgc gcgaaaacag
    48301 cattgcccat tttcttaccg gaaatatcca ccaccgcttt caacacctga ttgttttcca
    48361 gctcctgagc aatttcagtg gtcatatccg aaccgaaaac agcaattttt ccggcctgat
    48421 tttgattacg taccgctttt accgcgccga gtgtcgcacc gcccgattcc cccataatgg
    48481 cgttgagatc cggcgtggag ataattagtt tttcaccaac ggaaatcgct ttatctaaaa
    48541 cagtcccttc ctgattagcg acaatttgcg cgccgggaac gcgggatttt aatacttctt
    48601 caaatccttt acgtcgctgc acacaaactt caaaggcttc gcaattgatg acggcaattt
    48661 tcggctggtc aattttattg gcaataaaat aatcggcggc agcgttaccc agttttttac
    48721 caaattccag cggatcgccg accagatacg ccgagacata tttatcgacc cccttttgat
    48781 taatacaggt gttgtagcaa atcaccggaa tacccgcttc actggcgcga cgaacggtac
    48841 ggctactgcc attttcagac actgccgata aaataatggc atcgacatta cgcgccacga
    48901 gggtatcaac aaaggtactt tctttcgaaa tatcaccctg ggcgttagtt tcaattaact
    48961 gtacctgtac tgaagaatct tttgccgcat cctgaacgcc ctgacgcact ccagcgtaat
    49021 atccctgggt atcaaggtat attgcaccaa tggtcatttc tttatccgca gccctggcaa
    49081 atagtgcgct acctaatagc gtagccatta ataatagatt acgggttgtt ctcatttttt
    49141 taggcatagt gcctcctgta gggtttttat taacaacggc ttattctaat tattttgtga
    49201 tgagcggcag cgcgtgccgc tcaaatatta cagttaatag ggtttagtga acgtagttaa
    49261 aaataaaagg catcacggtg gtgaataatg ccgctttcgg ccaggccgga ttaatcgccg
    49321 tcaattcagc gcgagcagat tcatattgcg caacctcccc gagtcccgcg gacgccagca
    49381 tcgccaccat caggcatttt tctttatgct gctgttgtaa atcgccatac agcgacaaca
    49441 ggtcaggttg tgaaacagca aagaaatccg cctcaatact ggttttcgcc atctcttgcg
    49501 cccactgttt catttcacta aacagttgct gtgcggtttg ctgttcaccc agcagtcgca
    49561 gcgccattcc ttgccagaag agataatcaa ccggctgatc gttgtaataa ctgtggatgt
    49621 taatggtgcg atcgccggtc gccgccagac gtaaacaacg catcgcttca gtttcatcgc
    49681 cctgcgcgtt ggcgcatatc gcctgccaga accagatgtc gttatcagtt tgccccggta
    49741 aacggccttc gcttaaattc tccggataat gcagcgcggc atgaagcagt tcgctggcct
    49801 gctgcggctg tctggcatca agatgctgcc aggcgcgtaa taactggttg aggataaact
    49861 gactggtgac cttcccttcc ccgccttccc acgggtggaa tttgcgcgtg gcgagaatgt
    49921 ccgccgcttt gtctgcctga cccgtgagat gccacaaatt gagcagttct gcggtcatgt
    49981 cgtcgcgttt cagcgcaatt tccagattat tttccagacg cgccagtcgt ttctccggtg
    50041 tggctccact taacttatcc agcaaatccc gttcgaaaag cagacgtgca tcctgcggcg
    50101 caagctgata agcattatca agataacgcg cggccagttc ataatcgtgt tgcttattcc
    50161 acgcatggat cgccaacccg cgccagccat cggcaaactc cggcgacatc tctacgcaac
    50221 tttgccataa agtaatggct ttgttgtagc tgcgcttgtt gtagtagaag caggccagta
    50281 aatgacgggc aaaccagcac tcttcaatac tctccagcgc cgccacttct tccagcgtat
    50341 tcgggaaacg gacaaactgc gggaagacat caatggcttt tgcgaccagt tcgccgcgtt
    50401 cggctttcgg cagcaagctg gcttgtagat aaagcgataa cgtgcgctga cagtccagtg
    50461 cgttcagcat ctctgtcgcc agagtgggca ttccccagtt aatcagttgc ccggcggtca
    50521 tcagggcgtt aacgtcgcgt ccctgacaca gaccgcgcca ctgcgcgaga gctgattcgc
    50581 tacgaccatc gaaccagttc agccaccaca gagtggcgtt cagcggataa tcgcgcagca
    50641 gtttctcgcg ctgcacacgc gcgttgtcct gacgaccact taacaccagc agcagattat
    50701 gcaggcaaag cacttcctga ttggttgggc aggcacgaag actttgttgg caaaaatcca
    50761 gaccagcgtc gaagttacca ttacgcgccg ccagtcgtgc caggccataa tagccaccgg
    50821 ctttgctgtt gccgctccag accgcacgcc agaaatcctc ttcggcttgt tgatattgtc
    50881 cctgacgttc gtaagcactg gcgcgaatca aactcgcctg tccgcactgc ggatttttgt
    50941 tcagcgcatg tgcgcgtttc agagcctgag tggcatacgc caccgcttgc gggaaatcgg
    51001 cacggttata ttccagcatc gccagcgcca ggttacagcg gtaatccagc ggatccagcg
    51061 ccacgccgcg caggtagtaa tcgaacggtg aacggctggc gtgatgatat tgctccagat
    51121 gctgaccgat aaaccaggct tcatctgtac tggtaatgtc ttgtgctgac agtggcgctt
    51181 tggcgacgtc cggcaacggc aactcttgcg gctgatgttc ctgataactc aggacaatat
    51241 tgccgtcggc atcggagagc tcaatggtca gcctttcagg attgataccg tgcaacacgc
    51301 cctggatggc ggtcgcaggc atcagtgcca cggcatcatc aagtaacgcg ttgcatttgc
    51361 cgatttcgcg gatcgccagg cggtatccgt tcaacggaga gatggcatac agcccccact
    51421 caatcccccg ctcactacgc tggagtttta tcaccgcatc gcgggaggca ttttgcacca
    51481 tgcccaaaga atgataaggc aggaaatact gctcaaaacg cttctcttcg taagcatcaa
    51541 gccaggtaaa atcaggctgg ttatcggcaa aaataccggt catcagttcg atatacgggc
    51601 cgttattgtc ggtcagactc ttgtcccacg cctggccgaa ttcactgtgt ccccaactcc
    51661 actgtttttt acctggcgca atatggtggt tggcaacgtg cagcaaaccg ccatcttcat
    51721 cgtgacacca cgcgccgaca aaatcgtact gcgatttttc ggccatatat gaggttggaa
    51781 caggcacatt tttatagcga gaaatgtcca ctccggcgga gtagtccact ttgtagtaag
    51841 tgccggtggc gatggggaaa gcggagacgg cccgtttgcc gtgatcaaac accgcagtca
    51901 catccggtgg gaaaacgctc tgatgccctt cccccccttt cactgccggg ttggcccacc
    51961 acaagaaatg acgcggcgtg gcgttaccgt tatagacgcg gctggcgatt tccagcgccg
    52021 cccggtcagg gcgcagggtg aaacctgtca tcacctgtaa accatgcatc ggctccgttt
    52081 cgccgaccca caccgtctgt gcaccgtctt catgggcttc gagggtgaaa tcaacgggca
    52141 taaaggtggt cgggcgatgg tgttgcggcc agttaaactc aatcccaccg gagatccacg
    52201 gtcccagcag ccccaccagc gcaggtttaa tgacttcatt gtgataaaca aaatcgcgct
    52261 gtttcacttt atcccacgcg cgatgcaccc gaccgcccag ttccggcagg atcatcactt
    52321 tgatgtagtc gttttccagc cacaccgcct gccaggattt cagggttttc tgctcgctca
    52381 gcgtatcggt cacgccgtag ggataaaccg cgccggacga tccctgataa acgcgatttt
    52441 ccaggaacat gggatgtata tcctgcggcc cggtttcata ggtcgggatc tcaacgcgct
    52501 cttgccacac ttttactgga gtcatggtgc cctcaatatt aacaagacat actgaattaa
    52561 aagatttgtg gcagtgtatt gaacaatctg gcaatgtttt cgcggaataa tcacgcaatt
    52621 aactaaacaa ggtttagtga agatgagagc ctgcattaac aatcaacaga ttcgccacca
    52681 taacaaatgc gtgattctgg aactgctgta ccggcaaaag cgcgccaata aatcaacgct
    52741 ggcccggctg gcgcaaattt cgattccggc agtcagtaat attttgcagg aactggaaag
    52801 cgaaaaacgg gtggtgaata tcgacgatga aagccagacg cgcgggcata gtagcggtac
    52861 atggctgatt gcgccggaag gtgactggac gctgtgcctg aacgtgacgc ccaccagtat
    52921 tgagtgtcag gttgctaatg cttgtttaag tccgaaaggc gagtttgagt atttacagat
    52981 tgatgcaccg acgccgcagg cgctgctgtc cgaaatcgaa aaatgctggc atcgccaccg
    53041 taaattgtgg ccggaccgca ccatcaatct ggcgctggca atccacggtc aggttgatcc
    53101 ggtgaccggc gtgtcgcaaa ccatgccgca agcgccgtgg acaacgccgg tggaggtgaa
    53161 atatctgctg gaagagaagc tcggcattcg ggtgatggtc gataatgact gcgtgatgct
    53221 ggcgctggcg gagaaatggc aaaataattc gcaggaacgg gatttctgcg tgatcaacgt
    53281 tgattacggc attggctcgt cgttcgtgat taacgagcaa atttatcgcg gcagtttgta
    53341 tggtagcgga cagattggtc acaccatcgt taatccggat ggcgtcgtct gcgactgtgg
    53401 acgttacggc tgcctggaaa ccgtcgcctc gttaagcgca ttaaaaaaac aggcgcgggt
    53461 atggctaaaa tcacaaccgg ttaatactca gcttgatcct gaaaaattga ctaccgcgca
    53521 gttaatcgct gcctggcaga gtggagaacc gtggatcacc agctgggttg accgcagtgc
    53581 caatgccatt ggtttgagtc tgtataactt cctcaacatc ctcaatatta atcagatttg
    53641 gttgtacggt cgcagttgtg cctttggtga gaactggctt aatactatta ttcgccagac
    53701 gggatttaac ccgttcgacc gcgacgaagg accgagcgtg aaagcgacgc aaattggctt
    53761 cgggcaatta agccgcgcac aacaggtgct gggaattggc tatttgtatg ttgaggcgca
    53821 gttacgacag atttgatggc gcgataacgt agaaaggctt cccgaaggaa gccttttttc
    53881 aacagattaa ccgtttcgct tatgcgtaaa ccgggtaacg tgcgcagatg tcgagaactt
    53941 tacctttgat gcgctcgata acggcttcat cattgatgct gtccagcacg tcacacatcc
    54001 agccagccag ttctttcgct tctgcttctt tgaagccgcg acgcgtaatt gccggagtac
    54061 ccacgcgaat accggaggtc acaaacgggc tcttcggatc gttcggtacg ctgtttttgt
    54121 tgacggtgat gttagcacgg cccagagcgg cgtctgcttc tttaccggtc aggtttttat
    54181 caaccagatc aaccaggaac aggtggttat cagtgccgcc ggaaaccact ttgtagccgc
    54241 gttccaggaa cacttctacc atcgctttag cgtttttagc gacctgctgc tggtaagttt
    54301 tgaactcagg ctccatcgct tctttcagag caaccgcttt accggcgatt acgtgcatca
    54361 acggaccgcc ctgaccacca gggaaaacgg cagagttcag ttttttgtac agctcttcgc
    54421 taccaccttt cgccaggatc aggccgccgc gcggacccgc cagggttttg tgagtggtgg
    54481 tagtaacaac gtgagcatga ggaaccgggt tcgggtagac gccagcagca accaggcccg
    54541 caacgtgcgc catatcaacg aacaggtaag caccgatgct gtcagcgatt tcacgcattt
    54601 tcgcccagtc aaccacgccg gaatatgcag agaaaccacc gataatcatt ttcggcttgt
    54661 gttctttggc ttgtttttcc agatcggcgt agtcgatatg accggtagca tcgataccgt
    54721 aaggaacgat gttgtacagt ttaccggaga agttaaccgg agaaccgtga gtcaggtgac
    54781 cgccatgcgc caggttcata cccagaacgg tatcacctgg ttccagcagc gcggtgtaga
    54841 ccgcaaagtt agcctgggag ccggagtgcg gctggacgtt agcgtagtca gcgccgaaca
    54901 gttctttcgc acgatcgatc gccagttgtt caacgatatc aacatactcg caaccgccgt
    54961 agtagcgttt gcccggataa ccttcagcat atttgttggt cagctgagaa ccctgcgcct
    55021 gcattacgcg cgggctggtg tagttttcgg aggcgatcag ttcgatgtgc tcttcctgac
    55081 gtactttttc ctgctccata gcctgccaca gttcggcatc ataatcggca atgttcattt
    55141 cacgctttaa catccgcatc tcctgactca gctaacaata aaatttttgg cctttatagg
    55201 cggtcctgtt ggacaacggc gaacagtata accgaatcat tgtgcgataa caggtcttga
    55261 caaaggaatt tacgcaaacg attaccttca ggctacgcaa ggctttggag aataaagagc
    55321 ttgcaaccgg aaacggattt cttttcaggt ttgtgatgca aatttttcac ttcatcacat
    55381 tctttctgaa aaacaccaaa gaaccattta cattgcaggg ctatttttta taagatgcat
    55441 ttgagataca tcaattaaga tgcaaaaaaa ggaagaccat atgcttgacg ctcaaaccat
    55501 cgctacagta aaagccacca tccctttact ggtggaaacg gggccaaagt taaccgccca
    55561 tttctacgac cgtatgttta ctcataaccc agaactcaaa gaaattttta acatgagtaa
    55621 ccagcgtaat ggcgatcaac gtgaagcact gtttaacgct attgccgcct acgccagtaa
    55681 tattgaaaac ctgcctgcgc tgctgccagc ggtagaaaaa atcgcgcaga agcacaccag
    55741 cttccagatc aaaccggaac agtacaacat cgtcggtgaa cacctgttgg caacgctgga
    55801 cgaaatgttc agcccggggc aggaagtgct ggacgcgtgg ggtaaagcct atggtgtact
    55861 ggctaatgta tttatcaatc gcgaggcgga aatctataac gaaaacgcca gcaaagccgg
    55921 tggttgggaa ggtactcgcg atttccgcat tgtggctaaa acaccgcgca gcgcgcttat
    55981 caccagcttc gaactggagc cggtcgacgg tggcgcagtg gcagaatacc gtccggggca
    56041 atatctcggc gtctggctga agccggaagg tttcccgcat caggaaattc gtcagtactc
    56101 tttgactcgc aaaccggatg gcaaaggcta tcgtattgcg gtgaaacgcg aagagggtgg
    56161 gcaggtatcc aactggttgc acaatcacgc caatgttggc gatgtcgtga aactggtcgc
    56221 tccggcaggt gatttcttta tggctgtcgc agatgacaca ccagtgacgt taatctctgc
    56281 cggtgttggt caaacgccaa tgctggcaat gctcgacacg ctggcaaaag caggccacac
    56341 agcacaagtg aactggttcc atgcggcaga aaatggcgat gttcacgcct ttgccgatga
    56401 agttaaggaa ctggggcagt cactgccgcg ctttaccgcg cacacctggt atcgtcagcc
    56461 gagcgaagcc gatcgcgcta aaggtcagtt tgatagcgaa ggtctgatgg atttgagcaa
    56521 actggaaggt gcgttcagcg atccgacaat gcagttctat ctctgcggcc cggttggctt
    56581 catgcagttt accgcgaaac agttagtgga tctgggcgtg aagcaggaaa acattcatta
    56641 cgaatgcttt ggcccgcata aggtgctgta atttgatgtt gccggatgga aacatccggc
    56701 aacccttgac gcggtttaaa ttgccgcgtc gtcctcttca ccggtacgga tgcgaatgac
    56761 ccgtgccacg tcaaagacga agattttacc gtcaccgatt ttgccggttt gcgccgtgcg
    56821 aataatggta tcgacacagg tatcgacaat gtcgtccggt acgacaatct caattttcac
    56881 tttcggcaga aaatccacca tatactccgc gccgcggtac agctcggtat ggcctttctg
    56941 gcgaccaaag cctttcactt cggtcaccgt catgccggta ataccgactt cggccagtgc
    57001 ttcgcggaca tcgtccagct tgaagggttt tataatcgca tcaatctttt tcatgctatt
    57061 ccttgaaaag gtcgcctgtc ttttgatctg ctaaacgtaa cacataacgc caattcattc
    57121 cttgaaatcg tttgcatcca gctcgtgtcg ggaaagcagt ttataaaatt ctgtccggtt
    57181 gcgccccgcc attctcgccg cgtgggtgac gttgcctttg gtaatttgca gcagcttacg
    57241 caaatagttg agttcaaact gattacgtgc ctcaacaaag gttggcagcg ccgtgttttc
    57301 accctccagc gcctgctcta ctaatgcatc gctaataacc ggagatgagg tcagcgccac
    57361 gcactgttca atcacgttga ccaactggcg cacattaccc ggccaactag cggtcatcag
    57421 gcgtttcatc gcatcagtgg agaacgcgcg gacaaacggt ttatgtcgct ctgccgcctg
    57481 gcgcaacagg tgatttgcca gaagcggaat gtcttctgtg cgctctgcca gagccggaat
    57541 tttcaggctg acaacgttga ggcggtaata gagatcttcg cggaattccc cgcgcgccat
    57601 cgcttttggc agatcacggt gagtggcaga aataatccgc acgtcgatat caatatcgcg
    57661 gttgctgccc agcgggcgca ctttacgctc ctgcaacacg cgcagcagtt tgacctgtaa
    57721 cggcgcaggc atatcgccaa tttcatcgag aaatagcgtt ccgccttccg ccgcctggaa
    57781 taaaccttcg cgattgctga cagcgccagt aaacgcgcca cgcgcatgac caaacagctc
    57841 cgactccagc aattgctcgg gcaatgcgcc acagttaata gcaataaatg gtttgctatt
    57901 gcgcgggctg gcgttgtgga tagcctgggc gaaaatctct ttcccggtgc cgctctggcc
    57961 gttaatcaaa acgctaacgt ctgattgcgc caccagccgc gcctgttcca gcaaacgcag
    58021 catcagcggg ctgcgggtga caatcgcctc gcgccagcgt tcatcggtgg ctggcgcgga
    58081 ttgttccagc gcatcgtcaa ttgcctgata tagcgcatct ttatcgacag gcttggtgag
    58141 gaaactgaac acgccctgct gtgttgcagc aacggcatcg ggaatagaac catgcgcggt
    58201 aagaataatt actggcattc ccggctgcac tttctgaatt tcagcaaaca gctgcatacc
    58261 gtccatttca tccatccgca ggtcgctgat gactaaatct actttttcgc gattcagtac
    58321 ccgtaatcct tcagcgccac tttccgccgt gaccacgctg tagccttcgc tggtcaggcg
    58381 caggccaagc agtttcagca atcccggatc gtcatcgacc aataataaat gcgcaggttt
    58441 atggctcatc aggagtgacc tcatgggtgg atggcgcggg cttttcaccc tcgtgcggcg
    58501 tatccggcga gaaatttcca gccggtttgc gggtcgagag ctggcgttca atatcagtca
    58561 ggttttccag cttgcgggtg gtgagttcca gttgctgctg tagaacgtga tgttgctggc
    58621 gcaatgtatc cagctcgctg tcgctggcct gctggagctt gccgtagcgt tggcgctctt
    58681 ccgccagttg cagttgcagc gcctgaccgt cgcgccagag ttgatacaga gggcgaacct
    58741 gtgccgggat ctcggtactt agcgcatcaa tgcgcgcgac cagctggcgg cgctcatacg
    58801 gcgtaatttt ggcgtcggcg agcaaaatcc cttgtttaaa ggtattttgc cagctgccgt
    58861 cgtcatattg gcgagcttgc tgacgtgact gcgcaggcat taaacgatca gcacaatcca
    58921 tcgcccgcag ccagtaaagc ggattggttt cggttgattt accttgcagc gcccagatgt
    58981 cgctacattc agtagaaaga tagtcagcca gttgataaac cggaattttt tcttctgctg
    59041 gcgtatcaat ggctggctta ttgtgactct gcacgcaacc cagcaatgcc agacatggca
    59101 gccctgccag ccacagccgt cggggcaata atcgttgaaa aatgtgtcgc atattcacca
    59161 gacttaatgc ctatcccagt gggcgtaatt attgcaggca gtttggacat ggacagcgcg
    59221 gagaaaccgg agcgtacacg tcgtacgtga ggatttcgag cactgcccgg ggccgaactg
    59281 gcaaataaaa tagcctgatg ggataggctg ttagcattat ttcgtgtttt tcgacgacgg
    59341 caattcaatg cggaaacaaa cgtcttgccc gctctcgtcg accagataca gttccccttg
    59401 catacggcga atacaatccc tggcaatgct taatcccaga ccgctgccct tcaccgcccc
    59461 ttttcgctgg tggcttcctt gaaaaaaggg ttcgaagatc atggcgcgtt cctcttgcgg
    59521 aatgggtgtg cctgtattga tgacatcaat ataaacccgt gcaccatgta agctgctgcg
    59581 aaggcaaatg ttaccggatt cagccccgta gtgcaccgca ttggagtaaa gattatccag
    59641 tacgctcatc agcagcattg gctccgccag gcaagctgtt gctttgagat cgacgtcggt
    59701 atgcatcatt ttagcccgtg cgggcaggct atgagcagaa actactgtct ccaccagcgg
    59761 tgctaactca acattctcca gttccaccgc actgtccgct tgtttacggt tgtaatcaag
    59821 cagttgttcg atcagttttt gcaaattgcg gctgctgcta tcaagaatgc tcaccacctc
    59881 tttttgctct ggcgtaagcg gcccgacaac ctggtcagcc agtaattcag tgccctcgcg
    59941 catactcgcc agtggcgttt ttaattcatg agataaatgt cttaaaaatt gatggcgttg
    60001 ggattccagc catgacaggc gctcacttaa ccaaagaata cgttgcccaa ccgagcgtaa
    60061 ctcgctcggt ccactgaacg aaacgctatt gcccagagaa cgcccttccc ccagacggtt
    60121 gatcatgcgc tcgatatttt tcaccggccc gataatcatc cgcgtgaaaa gtagcaccat
    60181 caccagactc accagaaata gcaccagcga ttgccaacca aaatattgcc cacgttcggc
    60241 gatttcacgc tgaagttgct gcccacgaga gaacaccact gtgcgcgtgg cctgcaccat
    60301 ttcggtattg gcactggcaa aggcttcaag acgcgcggcg gcggcagcat cgggaccact
    60361 gttgttacac tgaagttggg ccagattgtt caaatcctga cgcaatgcct ggtaaagttt
    60421 atcgtctggc agtacgcctg cgtgggcatc gagcatttcg ctgtaacgct tgcgctggct
    60481 ttgataaacc ttcgcaagcg tagggtcgtc cagcacgcaa tactgacggt agctacgctc
    60541 catctccagc gccgcgttgg tcatcgcttc actacgccgg gcatcaataa gcgtagtgcg
    60601 gttaaccagc gccgcctgat cgctaagcgc attcaggctt tgccaggctt gccatgccag
    60661 caccaacagg ggtagtagaa ttagcaaaaa tgccagcatt accagttgtc gtaatgagcg
    60721 gggaaaaacg ggccagcgtt tcaaggtgtt actctcgtca gacgcgaata gcctgatgct
    60781 aaccgagggg aagttcagat acaacaaagc cgggaattac ccggctttgt tatggaataa
    60841 ggcggtgcct aactcgacgt ttcgcccgat ggttgatata gctacgctga tatcagaagt
    60901 tggacggcag gcaccttgtt gtgcgtcatt cgaattttat gtagcacgtc ccgaaggggc
    60961 tgacataagt cggtgaatga gccactgctt actattatgc agtaactgtg ccaataaaga
    61021 aaatagtttg gtaacgtact gattatacgt tgctttgagg gatttatggc ttcttcgcta
    61081 tttgaatgta aatcagccac tgtgtcgcca aaaagagaca acttaagata aacttattag
    61141 attacaataa aatcaatcat ttaagtgtcg ccagaaagcg acacggtaaa ccacccattg
    61201 tcgtgattta cagacacaaa aaagctcccg gagttgggag cttatgatag tggttggtgc
    61261 ttaatgccgg atgcggcgta aatgccgcat ccgggctgca aaaccaatga actgatgact
    61321 taccccaact gcttacgcgc attgcggaaa atgcgcatcc atgggccatc ctcgccccag
    61381 ttttccggat gccaggagtt gctgacagta cggaaaacac gttccgggtg cggcatcata
    61441 atggtgactc gaccactttc agtcgtaatc gacgtaatac cgttcggtga accgttcggg
    61501 ttagccgggt aggtttcagt gactttgccg aagttatcga catagcgcag tgccaccagc
    61561 cctttgcttt ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg cccttcacca
    61621 tgagagacag caatcggcat ttgcgagccc accatcccct gcaacagcag cgacgggctt
    61681 tgggtcactt caaccaggct gaaacgcgct tcaaagcgat cggaggtatt gcgcacaaaa
    61741 cgtggccaca actcactacc tgggatcagt tcacgcagat tagacatcat ctggcaaccg
    61801 ttacataccc ccagcgccag cgtttgcgga cggtggaaga aggttgcaaa ctcatcgcgt
    61861 acacggtcat tgaacaggat tgacttcgcc caaccttcac cggcacccag cacatcaccg
    61921 taggagaaac caccgcacgc gaccagggcg tggaaatctt ccaggcccgt gcgtccggtc
    61981 agcaggtcac tcatatgcac gtcgatagca tcaaagcctg cacggtggaa agctgccgcc
    62041 atttcaacat gcgagttcac gccctgctca cgcagcacag caactttcgg acgtgcgcca
    62101 gtggcaatat acggtgctgc cacatcttcg ttgatatcga acgacagttt tacattcagg
    62161 ccaggatcgg catcgttaga tttcgcctga tgctcctgat cggcacactc cgggttgtca
    62221 cgcaggcgct gcatctgcca ggtagtttct gcccaccaga cacgcaacgt agtgcggctt
    62281 tcgctgaata cagtctgccc gttggcggta atcacaaaac ggtcaccgga aaccgcctgc
    62341 ccaacataat ggacacaatc agcaagccca tgctgtgcca gtacggactc gaccgcttca
    62401 cggtcagcgg cacgaacctg aatcaccgca cccagttctt cgttaaacaa cgccgccagg
    62461 cgatcgtcac ccagagtggc gatatccgcg tcaatgccac aatgaccagc aaaggccatt
    62521 tccgccagcg ttaccagcag accgccatca gagcggtcgt gatacgccag cagcttacgc
    62581 tgtgcaacca gcgcctgaat cgcgtcatag aagcctttca gttgcgcgac gtcgcgtaca
    62641 tctgccggtt tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg
    62701 ttattgcctt tgcccaaatc aatcagcagc agtgcgttat cttcggtaga aagctgcggc
    62761 gtgatggtgt gacgtacatc ttccacgcgg gcaaatgcgg aaatcaccag cgacagcggc
    62821 gacgtcattt cgcgctcttc gttaccttcc tgccagcggg ttttcatcga catagagtct
    62881 ttacccaccg ggatcgtaag acccagcgcc ggacaaagct cttcacccac ggctttaacg
    62941 gcttcataca ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca gttggcggaa
    63001 agtttaatgc gtttgatatc accaatctgt gttgcggcaa tattggttaa cgcttcaccg
    63061 gccgccagac gggcagaggc ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc
    63121 gccatcgctt caccgtagta gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc
    63181 ggcacctgcc acggccccac catctgatcg cgcgctacca tgccggttac gctgcggtcg
    63241 ccaatggtca ccaggaaggt tttttccgcc acagtcggca gatgcagcac acgtttcacc
    63301 gcatctgcaa tggtgatccc ttcacgggcc agcgcgtcgc ctttcgcttt cagcgtttgt
    63361 acatcgcggg tcatcttcgg cgttttacca agcaggacgt ccagcggcag atcgatcggc
    63421 tgattatcaa aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc
    63481 accgcgtagg gcgcgcgttc acgcttacac agttcgtcaa acagcggtaa ctgatcggca
    63541 gcaaccgcca gcacgtagcg ttcctgggat tcgttacacc agatttccag cgggctcatg
    63601 cccggttcgt cgttcagaat atcgcgcagt tcgaatttac cgccgcgtcc gccgtcgctc
    63661 accagttccg gcatcgcgtt agaaagaccg cctgcgccga cgtcgtggat aaacagaatt
    63721 gggttagcat caccaagctg ccagcaacgg tcgatcactt cctggcagcg acgctccatc
    63781 tccgggttgt cgcgctgaac agaagcaaag tcgaggtcgg catcagactg accagacgcc
    63841 atagaagacg ccgcgccgcc gccaagaccg atgttcatcg ccgggccgcc gagaacgacc
    63901 agtttcgcac caacgttgat ctcgcctttt tgtacgtgat cggcacgaat gttgccgatc
    63961 ccgcccgcca gcatgatcgg tttgtgataa ccgcgcagct cttcgccgtt gtggctgttc
    64021 actttttctt cataagtacg gaagtagccg ttcagtgccg gacgaccaaa ttcgttgtta
    64081 aacgccgcac cgcccagcgg gccttcggtc atgatgtcca gcgcggtgac aatgcgctca
    64141 ggcttaccga aatcttcttc ccacggctgt tcgaagccag gaattcgcag gttggaaacg
    64201 gagaaaccaa ccaaacccgc tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg
    64261 atttcaccgc cggaaccggt cgccgcgccc ggccacggag aaatcgccgt cgggtggttg
    64321 tgagtttcga ctttcatcag aatatgcgcc ggttcctgat ggaaatcgta gcggcccgtt
    64381 tcgtggtcag caaagtagcg gcccacttca gaaccttcca ttacggcggc gttatcttta
    64441 taagcagaga gaacgtaatc tggcgtggtt tcgaaggtgt ttttgatcat cttaaacagc
    64501 gatttcggct gttgctcacc gtcgataacc caatcggcgt taaaaatctt gtggcggcag
    64561 tgctcggagt tcgcctgggc gaacatatac agctcgatgt cgttcgggtt acgaccaagc
    64621 tttgtgaaag catcctgcag atagtcaatt tcatcttccg ccagtgccag gccaagacgc
    64681 aggttagcgt cgattagcgc ctgacggccc tgccccagca aatcaacgct ggtgaccgga
    64741 gtcggttgat ggtgtgcaaa cagctgctct gcatcatcta aagcaaaaaa gaccgtttcc
    64801 atcatgcggt cgtgcagttc agcggtaacc tgctgccatt gttcattggt cagcgtaccg
    64861 gcttctatat agtaagcaac gccgcgctca aggcggttta cctgttgtag cccgcagtta
    64921 tgggcaatat cggtcgcttt cgaagaccag ggagagatgg tgccaggacg cggggtcacc
    64981 agcaggagtt tgccttgcgg ggcgtggctg gcgagtgccg gaccatattt cagcaggcgt
    65041 tcaagttgtg cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa atggacatac
    65101 tcggcgtaaa tattgtgaac cgggagcctg gcagcctgaa aacgtgccag cagtttgttg
    65161 attcggaatg ccgacagtgc aggcgaacca cgcagaattt ccatcataag tctctcgtct
    65221 tctaagcttt cggtgtaccc aaggggggga aacgggcgtc attataaaga atctgatgcg
    65281 ctgacgaaac cgtttgcgtg gaaataaaat caccatcgtg aattagcaac gcgtgccgcc
    65341 aatggctgta ataagttgcc atctggcgca ggtttacgca aaatgccgct catttatgag
    65401 taaacctttc actattatta cgttttttca agctgggacg cgcacgacac agagaattaa
    65461 ctaattgaaa aaattaaaga ttaattatct gttcatcggc attctggcac tgctgctcgc
    65521 ggtcgctctc tggccatcca ttccctggtt tggtaaagcc gacaaccgta tcgccgccat
    65581 tcaagcgcgg ggagagttgc gtgtgagcac cattcatact cccctgactt ataacgaaat
    65641 caacgggaaa ccttttggcc tggattacga actggcgaaa cagtttgccg attacctcgg
    65701 tgtaaaactg aaagtgaccg tgcggcagaa tatcagccag ctgtttgacg acctcgataa
    65761 tggtaacgcc gacctgctgg cggcaggact ggtctataac agtgagcggg taaaaaatta
    65821 tcagcctggc cctacctatt attccgtgtc acaacaactg gtttataaag tgggtcagta
    65881 tcgcccgcgc acactgggca acctgacggc ggagcaactc accgttgcac cgggtcacgt
    65941 ggtggttaac gatctccaaa ccctgaaaga cacaaaattc ccggaattaa gctggaaggt
    66001 agacgacaaa aaaggctctg cggaattaat ggaagatgtc atcgaaggaa aactcgatta
    66061 caccattgct gattctgtcg ccatcagcct gtttcagcgc gttcacccgg agctcgccgt
    66121 agcgctcgat atcaccgatg aacaaccggt gacctggttt agcccgttag atggcgataa
    66181 taccctttcc gccgccctgc tcgacttctt caacgaaatg aatgaagacg gtacgctggc
    66241 acgcattgaa gagaaatacc tagggcatgg cgatgatttt gattacgtcg atacgcgcac
    66301 atttttacgc gccgtcgatg cggtactgcc gcagttaaag cccctgtttg agaaatacgc
    66361 cgaagaaatt gactggcgtt tgctggccgc tattgcttat caggaatcgc actgggatgc
    66421 acaggccact tcaccgacgg gtgtgcgcgg catgatgatg ttaaccaaaa ataccgcgca
    66481 aagcctcggc attacggatc gtaccgatgc cgaacagagc atcagcggtg gcgtgcgtta
    66541 tttgcaggat atgatgagta aagtgccgga aagtgtgccg gagaacgagc ggatctggtt
    66601 tgccctcgct gcgtacaata tgggctatgc gcatatgctg gatgcccgcg ccctgacggc
    66661 aaaaaccaaa gggaatcctg acagttgggc tgacgtaaaa cagcgtctgc ctttacttag
    66721 ccagaaaccc tattacagca agctgactta cggctacgct cgtggacatg aagcctacgc
    66781 ttatgtcgaa aatattcgta agtatcagat tagcctggtg ggttatctgc aagagaaaga
    66841 gaagcaggct acagaagcgg cgatgcaact ggcgcaggat tatccggcgg tatcgcctac
    66901 ggagttgggc aaagagaaat ttccttttct ctcgtttctt tcccagtcgt catcaaacta
    66961 tttgacccat tctccctctc tgctgttttc caggaaaggg agtgaagaga aacaaaatta
    67021 atccgtcgag gattgcgctt ttttctgcgc tttaatttcc tggcggcgca tgcgaaagaa
    67081 gtcactgagc aacgccgcgc actcatccgc cagtattcct tccgtaattt ccactcggtg
    67141 attcataccc ggatgatgca gcacatccat taaagatccc gcagcgccag ttttcgcgtc
    67201 acgcgcacca aagaccacgc gaccaatgcg actgtggatc atcgctccgg cacacattac
    67261 acatggttca agcgtgacat acaacgtggc gtcgatcaga cgataatttt gcatcaccag
    67321 accaccctgc cgcagggcca tgatttctgc atgtgcggtg ggatcatggc gaccaatcgg
    67381 gcggttccag ccttcgccga ttacccgatt gttatgcact aataccgcgc cgaccggcac
    67441 ttcccgctca tcccaggcac gtttcgccag cgtcagcgcg tgacgcatcc agtattcgtg
    67501 gctaaattcg acttcagaca aaaagaaaac tccggttata aaagcgcggc gcattataca
    67561 cggacgctat gctttactct attccagttg ctggagttca ccgcgcgggg ttactcgcca
    67621 acgatgctga cagaaataaa gcagcggatt gtcctgatta ctgtcgctat agccactgta
    67681 cagccgcagc ggagtgccga ttttgcgctc cagttgtgcg accttttcat gtcccagaca
    67741 acgcatcgtc aatacccaac caccatagcc acgctgaatt tggctggcga taagattaac
    67801 ccgcggcagc cagggcgtat cgaaataaac cgcttcaacc agcggctgcg gagagccggt
    67861 aatcaaccag atatcagcat cggaacttaa caggtaggtg gttaatcgct cctgaaccag
    67921 cggaaaggcg gtaacattgt cgcgaaacca gcgcacgaaa tcggcctgca acgtctgtaa
    67981 acgtgcttcg ctgtgaccaa aagtgcaccc ccacagaagc agactcatcg gccagcgtgc
    68041 cgcacgacct tttatcaata acgcaatggc tataatcggt aacaacggca ggacaagtaa
    68101 cgcattcagc ggttggcgac gcagtaaata gcgcagaaaa ctgccgaaca tatcctgctg
    68161 atgcaatgtt ccatctaagt caaaaaacac cacacgacgc tcgtgagttg ccaaaccata
    68221 ctcctctgga ctgaaacatc ctgattaatt ttcgctacta catagcctaa cagatagatc
    68281 atcacttttc cggcaacaat cgaccgcatc atggctaact ggaattttta attcatggca
    68341 attagcggca atggaatata aaattcactc gcgtgtctct catattgcct gttgtcgcca
    68401 ctatttcatc gcgaggaaag atatgaacgg cttacttcgt atccgtcagc gttaccaggg
    68461 gcttgcccag agtgataaaa aactggcgga ttatctgctg ctacaacctg atacggcgcg
    68521 ccatttaagc tctcagcaac tggccaacga agccggagtc agtcagtcca gcgtcgtgaa
    68581 gttcgcgcaa aaactcggct ataaaggttt tccggcgctt aagttggcgt tgagtgaagc
    68641 gctggcaagc cagccggaat cgccctccgt gcccattcat aaccaaatcc gcggtgatga
    68701 tccgttacgg ctggtcggcg aaaaactgat taaagaaaat actgccgcta tgtacgcaac
    68761 gctaaacgtt aatagtgaag agaaactgca tgaatgcgta acaatgttgc gctctgcgcg
    68821 gcggataatt ctgaccggta ttggcgcttc gggtctggtg gcgcaaaact ttgcctggaa
    68881 gctgatgaag attggcttca atgctgccgc agtgcgcgat atgcatgcgc tactcgcaac
    68941 agtacaggcg tcgtcccctg acgatctgtt attagccatt tcctacaccg gtgtacgacg
    69001 cgagttaaac ctggcggcag atgagatgct gcgagtgggc ggaaaagtgc tggcgattac
    69061 cggctttact ccgaatgccc tgcaacagcg tgcttctcat tgcctgtata ccattgccga
    69121 agaacaagcg acaaacagcg cttcaatctc tgcttgtcac gctcagggaa tgttaacgga
    69181 tttgctgttc attgcgctga ttcagcagga tctggaactg gcaccagaac gtattcgtca
    69241 tagtgaagcg ctggtgaaaa aactggtctg agtaaagaat gcgcgtataa tgcccgcccg
    69301 gtttgtgttg ttttgagagt ttccttatgg cgttgttaat cactaaaaaa tgcatcaatt
    69361 gtgatatgtg tgaacccgaa tgcccgaatg aggcgatttc aatgggagat catatctacg
    69421 agattaacag cgataagtgt accgaatgcg tagggcacta cgagacacca acctgccaga
    69481 aggtgtgccc gatccccaat actattgtga aagatccggc gcatgtcgag acagaagaac
    69541 agttgtggga taaatttgtg ctgatgcacc acgcggataa aatttaatat tctactctgg
    69601 aagtagagta ttaattatat tactgggaag ccttaacgcc attatattta tttaattgat
    69661 gacattagca taatcattca ctaagttaat ttatatagta tctgccaaga cacttattta
    69721 tagttattaa aggcgcgtcc gattggttca ccggacgcac cttaagtacg tttccttgtg
    69781 ttataagaac agaaggatca gctgtaaaac agcaatgatg attttgatga cccgtttaat
    69841 caggtatcgg caatcagtca ttcgtttttc cttaaacaag gaatgcagct gttcagatta
    69901 gcccttacat ctccccaaaa ctgaacgtgc gagttattga gggtgcatgc tgcactccac
    69961 accagagctt tgacgacacc actcgtttca atgggggaat tctgtggcat ggtgtaaagc
    70021 acagcaaaat cttcaataac gaagccaatt ttaatgtact tacgaattgg cggtcacgtt
    70081 aatctttcca tcaatattgc tttcttcgta aaggctcgag tttttatgct aaagattgca
    70141 agttgcttgt aaaagataag tacactgatc cataatcgct gttgttgagg gtgcatgctg
    70201 cacaaaatta aagttaaaaa gtaaaacccc cgttccttac cagttcgggg gttttacttt
    70261 ttaaagagaa cggtattatt ttaactttca ataattaccg tggcacaagc atagtggcgt
    70321 tcatctgcca gcgttacatg catatttaca acgccgagct tttccgccag ttttaatgcc
    70381 tcaccccata gccgtagccg tggtttgccg agctcatcat tgaatacttc aaattgatta
    70441 aacgccagac cattgcggat cccggtgcca aacgcttttg ctgcggcttc tttcacagca
    70501 aaacgcttcg ccagaaaacg caccggctgg tggtgcgttt tccagattgc ccattcgtta
    70561 tcgcttaata cgcggcgtgc caggcgctca ccggatcggg cgatcactgc ttctatgcga
    70621 gcgatctcta cgatatccgt gcctaaacct aatattgcca ttagccacgc gcttccagca
    70681 tcagacgctt catttctgcc accgcatctt tcagtccggt catcactgca cgaccaataa
    70741 tggcatgacc gatattcagt tcatgcatct cagggatggc ggcaatagct ttcacgttgt
    70801 gataggtcag accgtgacca gcgttaactt tcagaccgag actagcggca aaggtcgcgg
    70861 ctttggcgat acgcgccagc tcttgcgcct gttcggcgtc agttttggca tcagcatagc
    70921 aaccggtgtg gatctcgata aacggtgcgc caacctctgc cgcagctttg atctgctctt
    70981 catcggcgtc gataaagaga gaaacctgga tcccggcatc tgccagacgt ttgcaggcat
    71041 cgcgcatttt gtcacgctgc cctgcgacgt ccaggccgcc ttcggttgtt acttcctgac
    71101 gcttttccgg taccagacag caaaaatgtg gcttcgtctc aacggcgatc gccagcatct
    71161 cttcggtcac cgccatctcc agattcatgc gggtatccag cgtctgacgc aggatgcgca
    71221 catcacggtc agtaatgtgg cggcgatctt cacgtaaatg cacggtaatg ccgtccgctc
    71281 ccgcctgctc agcaataaac gccgcctgca ccggatccgg gtaagcagta ccgcgtgcgt
    71341 tacgcagcgt tgcaatatgg tcaatgttga cgcctaacag taattcagcc atgacaatcc
    71401 tcatcattca taatgtgttt tcaccgttcg cttaggcata aactgccgga acagttccct
    71461 gctctttaat ggtttaccgc caagatacgg cttaagcgcc atgcgggtaa agcgtttcgc
    71521 ggcgcgtagt gtgtctgcgt caggaaattc ccgcgcgttt aacgctttta actgccttcc
    71581 ggtgaacgtt ttattgtcga taacgacgct tgcgataaac cctttttctt cgcgataacg
    71641 atacgtcatg gtgtcatcta ccggctcgcc gctacccgca caatgggtaa aattgacgcc
    71701 atagcccaga tgcccgagca gtgccagttc aaagcggcgc agcgcgggtt ctggcgtacc
    71761 agtggcacct gcaagagact gaatgcagtg caagtaatcg aaaaagagtt cagagaagcg
    71821 cgtctcgtat tccagtacgc gggagagaag ttcgttgatg tacagaccgc tgtaaagcgt
    71881 gataccgctt aatggcagcg ccagcgagac agcttcagca ctgcgcagcg ttttgacttc
    71941 gccacgcccg ccgaaacgta gcaagagagg ggtgaaaggc tgtaatgcac ctttcagagt
    72001 agagcgttta gagcgtgcgc ctttggcaac cagacgcacg cgacccgatt cctccgtgaa
    72061 gacgtccagc atcaggctgg tttcgctcca cgggcgacta tgcaggacaa atgcgcgctg
    72121 ccagccttcc atcggagtga ctcttaaaga tcgtcaacgt aaccgagact gcgcagtgcg
    72181 cgttcgtcgt cggcccaacc ggatttcact tttacccaca gttcaaggtg gacaggcgct
    72241 tcgaacattt cctgcatgtc tttacgcgct tcaatcccga tggttttgat cttggcccct
    72301 ttgttgccaa taaccatctt cttctgccct tcacgctcaa cgagaatcaa accattgatg
    72361 tcataaccac cgcgttcgtt agagacgaaa cgttcgatct ccacggtcac ggagtacggc
    72421 agttcggcgc cgaggaaacg catcagtttt tcgcggatga tttcagacgc cataaaacgc
    72481 tgtgagcgat cggtgatgta atcttccggg aagtgatggg tcgcttcagg gagatgctta
    72541 cgcacgattg ctgcgatagt gtcgacattc agcccggttt cagcagagat cggcacgata
    72601 tcgaggaagt tcatctgact tgccaggaac tgtaagtgcg gcagcagatc ggctttttcc
    72661 tgcacgttat ccactttgtt caccgcgagg atcaccggcg ctttgccatc gcgcagtttg
    72721 ttgagcacca tttcgtcgtc cggcgtccag cgggtgcctt caacgacaaa aatcaccagc
    72781 tcaacgtcgc caatagagct gctcgccgct ttgttcatca ggcggttaat agcgcgtttt
    72841 tcttccatat gcaggccagg ggtatcaacg tagatcgcct gatacgcgcc ttcagtatgg
    72901 atccccacaa tgcggtgacg agttgtttgc gctttgcggg aggtgatgga gatcttctgc
    72961 cccagcagtt tgttcaacaa tgtggatttg ccaacgttcg gacgtccgac gatggcaata
    73021 aatccgcagt aacttttatc gatgctcatt ccagctccag ttttttcaac gcctgttcgg
    73081 cggcagcctg ctcagcctta cgacggcttg aacctgtgcc aaccaccggt tcactcaggc
    73141 cgctgacctg gcagtggata gtaaattcct gatcgtgcgc ttcgccacgt acctggacta
    73201 ccagataagt cggcagcggc agatggcgac cctgcaaata ttcttgcaag cgcgttttcg
    73261 gatctttttg tttatcgcct gggctaattt cgtccaaacg agtttgatac cagttgagga
    73321 ttaatttctc gacggtttga atatcactgt cgaggaatac gccaccaatt aatgcttcga
    73381 cggtgtcggc gagaattgac tcacgacgaa atccaccgct tttaagttca cctggcccta
    73441 aacgtaagca ctcgcctaac tcaaattcgc gcgccagttc cgccagcgta ttgccacgga
    73501 ccagcgtggc gcgcatccgg ctcatatcgc cttcatccac acgagggaaa cggtgataaa
    73561 gcgcattggc gataacgtag ctcagaatag agtcgcctaa aaattctaaa cgctcgttat
    73621 gtttgctgct ggcactacga tgagttaatg cctgctgcaa cagttcctga tgattaaaag
    73681 tgtagcccag cttccgttga agccgattaa ttacgatggg gttcatgcga taccaataaa
    73741 taaatgcgtc aacaattcag cacacgaaac agacctgata tacatggctc tgctaactgc
    73801 ttcgctgcag tttcagttta ccggtatatg gggaccaacg ctgtttcgtg tgccgtggca
    73861 acctggaggt gccaacctta aacttcgggg gaatattcta tacacaacga cgggggatgt
    73921 cgttagccac gggagattta tctcataaat aattcacgtt gtcgccataa cggcgacaac
    73981 gtgaacgaag atgggctatt aatggatgcc gccaatgcga cttaagcgca cacctgtcgg
    74041 ccattcgcct tcttgcttat cgaaactcat ccagatagcc gttgcccgac cgaccagatt
    74101 cgcttcaggc acaaaccccc aataacggct gtccgcgctg ttgtcgcggt tgtcgcccat
    74161 catgaagtat tgtcccggag gaacaatcca ggttgccagt tgttgccctg gctgctggta
    74221 atacatcccc acctgatcct gcgcaatcgg cactgtcaga attcggtgcg tcacatcacc
    74281 cagtgtctct ttacgctcgg aaagacgaat tccattttct ttggtttcgt ttttcggcac
    74341 ttcaaagaat ccgctggtcg cttccccacc attacggcgt gagaaggtct gaacgaaatc
    74401 gctcggttcc acgtttgagt aggtgaccgg cagcgcgttt tcacacgcct ggccggaact
    74461 gcatcccggt tgaatcgtca gctctttcga gaccggatca taagtaactt tatcgcccgg
    74521 taaacccacc gcgcgcttga tgtaatcaag ctttggatct tccggatatt taaagaccac
    74581 gatatctccg cgtttcggat gaccggtttc gatcagcgtt ttctggtaga taggatcttt
    74641 aatgccataa gcaaactttt ctaccagaat aaaatcaccg attaacagag tcggcatcat
    74701 cgaacctgac gggatctgga acggttcata aataaacgaa cgcacaatca atacgatagc
    74761 cagtaccgga aaaacagaag caccggtttc cagccagcca ggcttcggcg caaccttttt
    74821 caacgttgct ttatccagtg agtcaccggc agccgcctgc gccgctgcct gacgttcccg
    74881 ccgtttaggt gcgaaaaaga atttatccac gcaccataaa atgcccgtca ccagtgtggc
    74941 aatcaccaga atcagggcaa acatattcgc catgccaact cctaagggtt atttgttgtc
    75001 tttgccgacg tgcagaatgg cgaggaacgc ttcctgcggc agctcgacgt taccgatctg
    75061 cttcatgcgt ttcttacctt ctttctgctt ctgcagcagc tttttcttac ggctgatatc
    75121 gccgccataa catttagcca gtacgttttt acgcagctgt ttcacggtgg atcgcgcaat
    75181 aatgtgcgta ccaatcgctg cctgaatggc gatatcaaac tgctggcgtg ggatcagatc
    75241 tttcatcttc tccaccaact cgcgaccgcg gttttgcgaa ttatcacggt gggtgatcag
    75301 cgccaacgca tcaacacgtt caccgttgat taatacgtct acacgtacca tgtcggacgc
    75361 ctggaagcgt ttgaagttgt aatccagaga cgcataacca cgcgaggtag atttcaggcg
    75421 atcgaagaag tcgagcacca cttccgccat cgggatctcg tacgtcagcg ccacctgatt
    75481 accgtggtaa accatattgg tctgcacgcc gcgtttttct acgcacagcg taataacgtt
    75541 gccgagatat gcctgcggca gcagcatgtg acattcggca atcggctcgc gcagttcgta
    75601 gatgttattt accgctggca gcttggatgg actgtcgacg taaataactt ctcttgacgt
    75661 ggtttcaact tcatacacta cggtcggcgc agtggtgatc agatccagat cgtattcacg
    75721 ttccagacgt tcctggatga tctccatgtg cagcaggccg aggaagccgc agcggaaacc
    75781 aaagcccagc gcgctggagc tttccggctc atagaacagt gaagcatcgt tcaggctgag
    75841 tttacccagc gcgtcacgga aggcttcata gtcgtcggaa cttaccggga acagaccggc
    75901 gtatacctgc ggtttgactt tcttaaagcc aggcagcgcc ttttctgccg gattacgcgc
    75961 cagcgttaag gtatcgccga ctggagcgcc gtggatatct ttaatcgcac ataccagcca
    76021 gcctacttcg ccacatttca gttcagtgcg gtcaacctgt ttcggcgtga agatgcccag
    76081 acggtcggcg ttataggtct gcccggtact catgactttc actttgtcgc ccttacgcag
    76141 ggtgccgttt ttaatacgga taagtgaaac aacgcccagg tagttgtcga accatgagtc
    76201 gataattagt gcctgcaacg ggccttccgg atcgccttcc ggcggcggaa tgtcgcgcac
    76261 cagacgttcg agaacgtcct gcacgccaac gccggttttc gctgaacagc gcaccgcgtc
    76321 agtggcgtcg atgccgacga tatcttcaat ttcttccgcc acgcgttcag gatcggctgc
    76381 cggcaggtca atcttgttca gtactggcac cacttcgaga tccatttcca tggcggtgta
    76441 gcagtttgcc agggtttgcg cttccacgcc ctgcccggcg tcgaccacca gcaatgcacc
    76501 ttcacaggca gccagcgaac gggaaacttc ataggagaag tctacgtggc ccggggtgtc
    76561 gataaagtta agctgatagg tttcgccgtc agacgctttg tagtccagcg tcacgctttg
    76621 cgctttgatg gtaatgccac gctcacgctc aagatccatg gaatcgagaa cctgcgcctc
    76681 catttcacgg tcagacaggc caccgcagat ctggataata cggtcagaca gcgtcgattt
    76741 accgtggtca atgtgagcta tgatcgaaaa gttacgtata ttcttcataa agtatgatta
    76801 ttgtgcctta atgcccggtt aaccaggctt ttagaagtcg ctgttctgag cttaacgtct
    76861 gtattaatag aaacgccgca ttctacacta caacattgag gcgaggaaat gttcataccg
    76921 tatggattgt ggtatctgga aacgtcctcg catttgttat gcaaaatgca acaaagccag
    76981 tgaaatcact ggctcgcgtc ttccgaagat gtttcaaatc gcacaaggcc aggcggcaat
    77041 gccacgctta agatgatcgg ttgccattct gcccgggcag caaacttacg cgagtagccg
    77101 cgcgcaatca ggaacccgcc aataccaccg aggatcgcac cacataatgc tgcgacgtca
    77161 gaagcaaaga gtagctgaaa tagcgaagcg ataaggaata atcccaccag cggcgacata
    77221 taaaccagta atgcggagct aagcaggctg ccttcggcga tccccaattc cactttttgc
    77281 cccggcacta acggttcatc acagggtacg acaatggtat gcgtggtttg cgggccaagt
    77341 ttattcaaca cgcggctacc gcaaccggcg cgtgaagcgc agctgctgca tgaagcttta
    77401 acatcacaac tgaccagcgc ctgcccgttt tgccaggaga cgacggtagc ccactctttg
    77461 atcattgcgc tgccccgaac ttaatattct cggcaatgcg tttcgccgtt tgcggcggca
    77521 gttcaccgac aatggtgatt tcggcgttat cacgtacgct tgtactgacg gttctgcgtc
    77581 cggtgcgcaa catctgatcg gtgctcgatg gcgtagcgcg gttaacgttt accgagaagc
    77641 tgaataatcc gtcggaatag agacgtgatt cgataggcat gttgtccatc gtcggtagcg
    77701 gacgtcgact actggaaact tcgctaaaac cctgtggcaa ccaggttggc gtccagctga
    77761 atttagcttt ttcacctaca ggaacagaaa gcaacggcgg caaatttgcc ttcgccagcg
    77821 tctgcatact gctactgata tcctgattga cgttaaaagc aatcacgcga aattgttcca
    77881 gcgtttcacc atcgcgatca aggagatcaa ccctcatcgg taatttcgat tcgatgtcca
    77941 tccacacgat gtagctgtag cgtgtaccat ctcgggcaac cacgcgaatg acttcgcaaa
    78001 gacgatcagc aatacgcgtg cgcccgacgg agataaagtc gtagtaagga gaaaggcgtt
    78061 tgaaatcggt atagatgagc gatggcagag aatcaacaat gtaatcgcca ttaagcgtga
    78121 acggttcaag tcccggttca aaatagctga tttcattgcc acgctgtacc acttcccggc
    78181 gcgggccatc catttgcaac aattgtgcaa gaggacggtt atcgaggcgt gcatgtcgat
    78241 aacgcagaga ctcaacaccc tgtttattga tgctgatgaa tgacagctcg taattcagtg
    78301 actgactggc caggttcatc tgctgtaata acgccccgga cgcgggagtg gccgaggcgt
    78361 tagcagagaa taacaggcta cctgtcacta atgacatggc aaaccaaagt tgcttcatta
    78421 ctgcgattgc gttcctaaag tttgaattcc tggcacctgt acagcggctt gctgggtttg
    78481 cgcctgctca aactgaagct gttcagagtg gagtcggcgt tgcagttcgt aatcctgcaa
    78541 cattgcatta atgcgacgac gctgctcctg tacctgctgc tgttgaccat tgtttgcggt
    78601 cgcttcagaa ggtactccca ggcttaccgg gctggcttta cccatcatcg gcagtgtatt
    78661 aaataccggc gtttcgggct gctgggacgt ttcagattgt ccattatagt gctggacgcc
    78721 aacgataact gcaagcgata cgcatgctgc tacgcccatt tgggtaagct gtgccgccca
    78781 cggacgtact ttctgccaga atggcatttt ctgccattga tgcggcgcag gctgggcttc
    78841 cgggatcaat gtcgccggtt gacgtactgg ctcttcttca atggcggcca tcacgcgtga
    78901 agagatatcg aaatggagca cctcgggagt atcaccccgc attgagtcac ggattaagtg
    78961 atagctttcc caggttttct gcatttctgg gttatgagcc agttcgttaa gcagctcact
    79021 atccagcgtt tcgccatcca ttaaagcgga aagttgttct ttctgcatgc ctaataccct
    79081 tatccagtat cccgctatcg tcaacgcctg ataagcggtt gaactttgtt atcaatagct
    79141 tccctcgctc ggaagatacg tgaacgcacc gtacctaccg gacaatccat gatagcggct
    79201 atctcttcat agctcaggcc atccagctcc cgcaaggtta ttgccatgcg caaatcttcc
    79261 gggagggact caatagttcg gaaaactatc tgtctcagtt cttctgacaa cattaagttc
    79321 tcagggttcg aaatttcttt caacgcgccg ccactttcga agttttcagc ttcaatggca
    79381 tccacatcac tggaaggtgg acgacgcccc tgagcaacca ggtaattttt cgctgtattt
    79441 acagcaatcc gatacagcca tgtataaaaa gcgctatctc cccggaacga atccagcgca
    79501 cgataggctt taataaaagc ttcttgtacc acatcgggaa catcacccga cggcacatag
    79561 cgggaaacca gactcgccac tttatgctga tagcgcacta ccagtaggtt aaaggctttc
    79621 tgatctccct tctggacccg ttcaaccagg acctggtccg ttaactgctc gctcatccga
    79681 ggtaaagtct ccccaaacca aatttccacg cgctatcgaa acgccactcc attagctgca
    79741 atttgagcaa gcaaagggtt agagtgtctc gtttttgtaa agttccgtaa cgcatctgtt
    79801 tttgtttgtc atgctgtaga cggatcatta tctatcatta taagtctaca gaatctgaac
    79861 atcgcattat ctgtgtagaa atgcccattt aactgcctga agagtaaccc aacggccttt
    79921 ttatttcacc acctaatcct ccaccagcca gtaacttctc tttttctcgc cgccctgcgt
    79981 cagcgtgttt agccactgta acaaatatta aaatagcagg tgtttattcg cacaacatga
    80041 tgctatgctg accaaacggt gtttagtaaa ttaaacaaag aaaatgaata ctctcccaga
    80101 acattcatgt gacgtgttga ttatcggtag cggcgcagcc ggactttcac tggcgctacg
    80161 cctggctgac cagcatcagg tcatcgttct aagtaaaggc ccggtaacgg aaggttcaac
    80221 attttatgcc cagggcggta ttgccgccgt gtttgatgaa actgacagca ttgactcgca
    80281 tgtggaagac acattgattg ccggggctgg tatttgcgat cgccatgcag ttgaatttgt
    80341 cgccagcaat gcacgatcct gtgtgcaatg gttaattgac caaggggttt tgtttgatac
    80401 ccacgttcaa ccgaatggcg aagaaagcta ccatctgacc cgtgaaggtg gacatagtca
    80461 ccgtcgtatt cttcatgccg ccgacgccac cggtagagaa gtacaatcca cgctggtgag
    80521 caaggcgcag aaccatccga atattcgtgt gctggagcgc agcaacgcgg ttgatctgat
    80581 tgtctctgac aaaattggcc tgccgggcac gcgacgggtt gtaggcgcgt gggtatggaa
    80641 ccgaaataaa gaaacggtgg aaacctgcca cgcaaaagca gtggtactgg caaccggcgg
    80701 tgcgtcgaag gtgtatcagt acaccaccaa tccggatatt tcttctggcg atggcattgc
    80761 tatggcgtgg cgcgcgggct gtcgggttgc caatctcgaa tttaatcagt tccaccctac
    80821 cgcactgtat cacccacagg cacgcaattt cctgttaacg gaagcactgc gcggcgaagg
    80881 cgcttatctc aagcgcccgg atggcacgcg ttttatgccc gattttgatg agcgcggcga
    80941 actggccccg cgcgatattg tcgcccgcgc cattgaccat gaaatgaaac gcctcggcgc
    81001 agattgtatg ttcctcgaca tcagccataa gcccgccgat tttattcgcc agcatttccc
    81061 gatgatttat gaaaaattgc tcgggctggg gattgatctc acacaagaac cgataccgat
    81121 tgtgcctgct gcacactata cctgcggtgg tgtaatggtt gatgatcatg ggcgtacgga
    81181 cgtcgagggc ttgtatgcca ttggcgaggt gagttatacc ggcttacacg gcgctaaccg
    81241 catggcctcg aattcattgc tggagtgtct ggtctatggc tggtcggcgg cagaagatat
    81301 caccagacgt atgccttatg cccacggcgt cagtacgtta ccgccgtggg atgaaagccg
    81361 cgttgagaac cctgacgaac gggtagtaat tcagcataac tggcacgagc tacggctgtt
    81421 tatgtgggat tacgttggca ttgtgcgcac aacgaagcgc ctggaacgcg ccctgcggcg
    81481 gataaccatg ctccaacaag aaatagacga atattacgcc catttccgcg tctcaaataa
    81541 tttgctggag ctgcgtaatc tggtgcaggt tgcggaattg attgttcgct gtgcgatgat
    81601 gcgcaaggag agtcgtggcc tgcatttcac gctggattat ccggaactgc tcacccattc
    81661 cggtccgtcg atcctttccc ccggcaatca ttacataaac agataaaaag cctgggtcag
    81721 cgccgtatac gcttcggaat agttctggtc tggcccacga atgactaagc gatcgctaaa
    81781 gcattctccc gcctgcgggg agaatgccag cagcacccga tgcggcagtc gcgcttcgtt
    81841 ttccgccaca tccgtccgca aacgtaaatg ccagcccatg cttaatgcca gctccgtaaa
    81901 accattacca atctgctctg gcagcactac gcagaaaaat ccctcttcgg taatgcactc
    81961 cgccgcacag gtcagcagcg atgggtgatc aagcgtagtg gtatagcgag cctgttcccg
    82021 ttgaggggtc gcgcactcta ctccctgctg atagtaaggt gggttactga tgattaaatc
    82081 gaagcgtact gtctgctgtg tgatccactg ctgaatatcc gccgtatgga cgttaatccg
    82141 ctctgcccac ggggactggt tgatattttc ctgcgcctgc gcggcagctt cactttccag
    82201 ctcaacggca tcaattatca cgctgtcatc ggttcgctgc gccagcatta atgccagcaa
    82261 cccgctaccc gcgccgatat caaggcaacg tttcacccca gccaccggtg cccatgcgcc
    82321 cagcaaaatg ccatccgttc ccactttcat cgcacagcga tcgtgagcaa caaaaaactg
    82381 tttaaaagta aatccattac gacgaagcac ggatgtagac tgtgacatga aaataaaacc
    82441 ttgcaggaaa aacggcgata gcaccgggtg agaacaatac ccgagaagcg atatccatac
    82501 aaacagatga agattgcagc cgtaacgtct ataatcagcg ccccacacag aggtagaaca
    82561 tgactgtaac gactttttcc gaacttgaac tcgacgaaag cctgctggaa gccctccagg
    82621 ataaaggttt cactcgcccg accgccattc aggctgccgc cattccgcct gcgctcgatg
    82681 gccgtgatgt actcggttct gcgccgacag gcaccggtaa aacggcggcg tatctgctgc
    82741 cagcgttgca gcacctgctc gatttcccgc gtaagaaatc cggtccgccg cgtattttga
    82801 tcctcacccc aactcgcgag ctggcgatgc aggtggccga tcatgcccgc gaactggcga
    82861 aacatacgca tctggatatc gccaccatca ccggcggcgt agcctatatg aaccacgcgg
    82921 aagtgttcag cgaaaatcag gacatcgtgg tcgccacgac cggacgtctg ctgcaataca
    82981 taaaagaaga gaacttcgat tgccgcgcgg ttgaaacgct gatcctcgac gaagcagacc
    83041 gtatgctgga tatgggcttc gctcaggata tcgaacatat tgctggcgaa acgcgctggc
    83101 gtaaacagac cctgctcttt tcggcaacgc tggaaggcga tgcgattcag gactttgccg
    83161 agcgtctgct ggaagatccg gtggaggttt ctgccaatcc gtccacccgt gagcgcaaaa
    83221 aaattcatca gtggtattac cgcgccgatg acctggaaca taaaaccgca ttgctggtgc
    83281 atctgttaaa acagccggaa gcgacccgct caattgtgtt tgtgcgtaag cgtgaacgtg
    83341 tgcatgagct ggcaaactgg ctgcgcgaag cgggcatcaa caactgctat ctcgaaggtg
    83401 agatggtaca aggcaagcgt aacgaagcga tcaagcgttt gaccgaaggt cgcgtaaacg
    83461 tactggttgc aaccgatgtt gccgcgcgcg gtatcgacat tcctgacgtc agccacgtct
    83521 ttaacttcga tatgccgcgc agtggcgata cttatttgca ccgtatcgga cgtaccgcgc
    83581 gcgccggtcg taaaggcacc gcgatttcgc tggtggaagc ccatgaccat ttgctgctgg
    83641 gcaaagttgg ccgttatatt gaagagccaa ttaaagctcg cgttattgat gagttacgcc
    83701 cgaaaacgcg tgcgccaagc gagaagcaga ccggcaagcc atcgaagaaa gtactggcaa
    83761 aacgcgccga gaagaaaaaa gttaaagaga aagagaagcc gcgagtgaaa aaacgccatc
    83821 gcgataccaa aaatattggt aagcgccgta aaccaagcgg aacgggcgtg ccaccgcaaa
    83881 cgacagaaga gtaatctcaa tgccgggttt aagcccggca ttatttttat ccagccacaa
    83941 aacactcttc aacacactga ataaaagtgt gcatcgccgg actaaccgct tttccggcat
    84001 ggtgagcgca cattgccgta atggtctgcg tctgttcgcc aaaaggcaat tcgattaatt
    84061 cgccactcgc aagttcattt cccactgcaa agcgcggcag gtaactgacc ccgatatttg
    84121 ccgcgacaca acgcttgatg ctttcgatac ttataagctc aatggtgttt tccaccgtga
    84181 tccgccgctg gcgcagcgtg ctctcaaata tctgccggaa gacacattgt ggttcgttga
    84241 taataaagct acaggcgtta tgtcttcccg gctcagtaaa atcgatatct gcaatttgcg
    84301 gtgaagccac cagcaccagc ggttgttcac ccaactctcg tcggttcaga acatcatcat
    84361 tccctacgcg ataaaaaacc ccgacatcgg cctcatcatt cagtagcgca tcacggatca
    84421 cgtaacagtt cagcgactgc aacgataaac gcacttttgg agcgcgttgc cgaaaacgct
    84481 gcaacacctg cggcattcgg taagagagca acgtttcgcc tgaaacaacg cgtagttccc
    84541 cgtccggatc cgactctttt ttcgcagcct cacgaagcgt atccatcact cgggtaagtt
    84601 catagatgtg cggcagcaac ttttttcctt cgcgggtaag gcacattcgt cgaccaattt
    84661 tctcaaacaa ttgtaccgag aactcctgct caagctgctg aatatgaaaa gtcaccgtcg
    84721 attgtgtaca gcacaatttt tgcgaagctc gcaaaaagga accctcttcc accacggttt
    84781 taagtgtaat aaaacggcgc agatccataa ccccaaacct atcgaaaata tcgaatttag
    84841 aatgtaaaaa cattcatttt tttaaatgtt ccgtgtcggg tactgtctac caaaacagag
    84901 gagataacaa gtgacaccga cccttttaag tgctttttgg acttacaccc tgattaccgc
    84961 catgacgcca ggaccgaaca atattctcgc ccttagctct gctacgtcgc atggatttcg
    85021 tcaaagtacc cgcgtgctgg cagggatgag tctgggattt ttgattgtga tgttactgtg
    85081 tgcgggcatt tcattttcac tggcagtgat tgacccggca gcggtacacc ttttgagttg
    85141 ggcgggggcg gcatatattg tctggctggc gtggaaaatc gccaccagcc caacaaagga
    85201 agacggactt caggcaaaac caatcagctt ttgggccagc tttgctttgc agtttgtgaa
    85261 cgtcaaaatc attttgtacg gtgttacggc actgtcgacg tttgttctgc cgcaaacaca
    85321 ggcgctaagc tgggtagttg gcgtcagcgt tttgctggcg atgattggga cgtttggtaa
    85381 tgtgtgctgg gcgctggcgg ggcatctgtt tcagcgattg tttcgccagt atggtcgcca
    85441 gttaaatatc gtgctcgcgc tgctgctgat ctattgcgcg gtacgcattt tctattaatt
    85501 aaaaaagcgg aagaggtcgc cctcttccgc ttagtaactt gctacttaag ccttacaggc
    85561 tttcagtaaa ggtacgagcg ataacgtcgc gctgctgttc cggagtcaga gagttaaagc
    85621 gaactgcata acctgataca cggatggtca gctgcggata tttttccgga tgcttaactg
    85681 catcttccag agtttcacga cgcagaacgt taacgttcag gtgttgacca ccttcaacgc
    85741 gaacttctgg tttcacttct actggaactt cacggtattc aatgtcaccc agtttgctta
    85801 ctgcaaccac ttcatcttct gcataacctg cttttgcaac gatgcaacgc gcttcgcctt
    85861 tttcgctgtc cagcagccag aaagagttca gcagatcgtc gttagcggct ttagtaatct
    85921 ggatacctgt aatcatgtga tgcctccccg gcaaaattat ttgatttatt cagcctgtcg
    85981 cggccaattg gtaaaaccat tgttgcttga gtgtatatat actcctcaac aacccttgat
    86041 tccttgattt aaatcaataa aaaccacaca tcgattgtgg tcgcaaatgg attttattgt
    86101 tttacatcaa cttatgcggg tgtgaaattt taccaattta catttttttg cactcgttta
    86161 agtctaaaaa atgagcatga ttttgttctg tagaaataag cagttaagct aggcggattg
    86221 aagattcgca ggagagcgag atggctaacg aattaacctg gcatgacgtg ctggctgaag
    86281 agaagaagca accctatttt cttaataccc ttcagaccgt cgccagcgag cggcagtccg
    86341 gcgtcactat ctatccacca caaaaagatg tctttaacgc gttccgcttt accgagttgg
    86401 gtgacgttaa agtggtgatt ctcggccagg atccttatca cggaccggga caggcgcatg
    86461 gtctggcatt ttccgttcgt cccggcattg ccattcctcc gtcattattg aatatgtata
    86521 aagagctgga aaatactatt ccgggcttca cccgccctaa tcatggctat ctcgaaagct
    86581 gggcgcgtca gggcgttctg ctgctcaata ctgtgttgac ggttcgcgca ggtcaggcgc
    86641 attcccacgc cagcctcggt tgggagacgt ttactgataa agtcatcagc ctgattaacc
    86701 agcaccgcga aggcgtggtg tttttgttgt ggggatcaca tgcgcaaaag aaaggggcga
    86761 ttatagataa gcaacgccat catgtactga aagcaccgca tccgtcgccg ctttcggcgc
    86821 atcgtggatt ctttggctgc aaccattttg tgctggcaaa tcagtggctg gaacaacgtg
    86881 gcgagacgcc gattgactgg atgccagtat taccggcaga gagtgagtaa atttgcgggg
    86941 aaatgccgga tggcagagtt gccacccggc tgatttatca ggctttattc tgacgccacc
    87001 attcaccaag caaaacgccg gttgcgacag agatattcag cccggcaacg ttgcccgtac
    87061 cgtcaatctt cacgcgcaga tcgttcggat cgcgtgcggc atccggtaac ccttcatatt
    87121 cctgacccag caccagtacc attttcgctg gcagactagt tttgaacagc ggtttaccct
    87181 gctcgctgga agtggtcact acggtgtaac ctgcctgacg gaaatcatcc agcacgttaa
    87241 caatgttgtc gccagtaatc ggctgaacgt gctctgcgcc gccttctgcg gtacggatag
    87301 ccgcccccga ctccagcagt gccgcatcct gcaccacgac acctttcacg ccgaagtgcg
    87361 cgcagctgcg catcatgcca cccaggttat gcgggttaga ttcgttttcc agtgccagaa
    87421 cgcaatcctg cgcgcctgcc tggcttaccc actgctgcac ggttgtaccg ttacgctttt
    87481 tgatcaagaa gcaaacgccg ccgtgatgtt ccgtgcctga cgcttttgtc aattccgctt
    87541 catccaccac atggtacgct ttgcggtttg ctgccatcca gcgcaaggct tctttaaaac
    87601 gcggcgttac actctggata aaccaggcgc gaacaatcgc ttccggacgg ctctggaaaa
    87661 gtgcctgaca tgcattttcg ccgtagacgc gggtttcttc cgcacgctga cgacgcaaca
    87721 cttccggatc aataaaactt ttaccactga tgccaccgtg atcggccttt tccggcgtct
    87781 catcacccgg cgcgcgggaa accgtgcgcc acggcgaatc ttcccacttg cggtcacggg
    87841 gctgactctg tttgtcatcg cgggcggggc gacggccacc gtcagcacga gattttcctg
    87901 gacgcccgcc ccctttcccg gtacgcgggt tgtgggtacg tttatcagaa tcatcatcac
    87961 tgcggacata catcactttg accttgccgc ttttaccttt catttcatcg ttcatgcttt
    88021 tctccaccag cgctgcgcga agcgcgcaga ttacccgaag tccgcgcggt tcgccatgat
    88081 ttcgtaccaa agcctgcgac tatcatacct attgaataaa acagattgtt gtctggaaca
    88141 atgtccccga taatatgtaa catattagaa acataccggc gtcgttgccg ataagtctcc
    88201 ttactcatcc cgaggttagt tatgaatacc gtttgtaccc attgtcaggc catcaatcgc
    88261 attcccgacg atcggatcga agatgcggca aaatgcggac gctgcggtca cgacttgttt
    88321 gacggagagg tgattaatgc gaccggtgaa acactcgaca aattgctgaa ggatgatcta
    88381 cctgtggtga tcgacttctg ggcaccgtgg tgcggcccct gccgtaattt cgcaccaatt
    88441 tttgaagatg tcgcgcaaga gcgtagcggt aaagtgcgct ttgtgaaagt gaataccgaa
    88501 gctgaacgtg aattgagcag tcgctttgga attcgtagta taccgacgat catgattttc
    88561 aaaaacggtc aggttgtcga catgcttaat ggcgcagtac cgaaagcgcc gttcgatagc
    88621 tggctgaacg aatctcttta atcttaccgg ggcacatctt gtgccccgtt ttctcctctg
    88681 cgacaatggc gttttttcga cgctctctta tgaccgaaaa cgctgttctc cagttacgcg
    88741 ccgagcgtat tgcgcgcgca acacgtcctt ttcttgcccg cggtaatcgc gttcgtcgct
    88801 gccaacgctg tcttttgcca gagaaattat gtctctgttc gacaattacc ccagcacaag
    88861 caaaaagtcg cttctgtttg ctgatgttcg acaccgagcc aatgaagcca agtaacaccg
    88921 ggcgtctgat tgctgatatt ttgcctgata ccgttgcgtt tcaatggtcg cgtaccgacc
    88981 cctcgcaaga cttgctggat ctggtacaaa acccggacta tcaaccaatg gtggtctttc
    89041 ccgcctcgta tgctgatgag caacgggaag tgatcttcac accacctgcc ggtaagccac
    89101 cgctgtttat catgctcgat ggtacctggc cggaagcgcg caagatgttt cgtaaaagtc
    89161 cgtatctgga taatcttccc gtcatttccg tcgatctttc ccggctttct gcctatcgcc
    89221 tgcgtgaagc ccaggctgaa ggccaatatt gtactgccga ggtagccatc gcactgttag
    89281 atatggccgg cgataccggg gcggcagcag ggttaggcga gctttttacc cgctttaaaa
    89341 cacgctatct ggcaggaaaa acgcaacatc tgggtagcat cacagcagaa cagttagaaa
    89401 gcgtttaaaa tcattcggtc acttctgcgg gagaccggta tgagtcagcg aggactggaa
    89461 gcactactgc gaccaaaatc gatagcggta attggcgcat cgatgaaacc caatcgcgca
    89521 ggttacctga tgatgcgtaa cctgctggcg ggaggcttta acggaccggt acttccggtg
    89581 acgccagcct ggaaagcggt gttgggtgtg ttagcctggc cggatattgc cagcttgccc
    89641 tttacacccg accttgcggt tttatgtacc aatgccagcc gtaatcttgc tcttctggaa
    89701 gagctcggcg agaaaggctg taaaacctgc attattcttt ccgccccggc atcgcaacat
    89761 gaagatctcc gcgcctgcgc cctgcgccat aacatgcgcc tgctaggacc aaacagtctg
    89821 ggtttactgg ctccctggca aggtctgaat gccagctttt cgcctgtgcc gattaaacgc
    89881 ggcaagctgg cgtttatttc acaatcggct gccgtctcca acaccatcct cgactgggcg
    89941 caacagcgtg agatgggctt ttcctacttt attgcgctcg gcgacagcct ggatatcgac
    90001 gttgatgaat tgctcgacta tcttgcgcgc gacagtaaaa ccagcgccat cctgctctat
    90061 ctcgaacagt taagcgacgc gcgacgcttt gtttcggcgg cccgtagtgc ctcgcgtaat
    90121 aaaccgattc tggtgattaa aagcggacgt agcccggcgg cacagcgact gctcaacacg
    90181 acggcaggaa tggatccggc gtgggatgcg gctattcagc gtgccggttt gttgcgggta
    90241 caggacaccc acgagctgtt ttcggcggtg gaaaccctta gccatatgcg cccgctgcgt
    90301 ggcgaccggt tgatgattat cagcaacggt gctgcgcctg ccgcgctggc gctggatgcc
    90361 ttatggtcac acaatggcaa gctggcaacg ctaagcgaag aaacctgcca gaaactgcgc
    90421 gatgcactgc ctggacatgt ggcaatctct aacccgctcg atctgcgcga tgacgccagc
    90481 agcgagcatt atgtcaaaac gctggacatt ctgctccaca gccaggattt tgatgcgctg
    90541 atggttattc attcacccag cgcagcggct cccgcaacag aaagcgcgca agaattaatt
    90601 gaagcggtaa agcatcatcc ccgcagcaaa tatgtctctt tgctgacgaa ctggtgcggc
    90661 gagcactcct cgcaagaggc acgacgttta ttcagcgaag ccgggctgcc gacctatcgc
    90721 acgccggaag gcaccatcac cgcgtttatg catatggtgg agtaccggcg taatcagaag
    90781 caactacgcg aaacgccggc gttgcccagc aatctgactt ccaataccgc agaagcgcat
    90841 cttctgttgc aacaggcgat tgccgaaggg gctacgtcgc tcgataccca tgaagttcag
    90901 cctatcctgc aagcgtatgg catgaacacg ctccccacct ggattgccag cgatagcacc
    90961 gaagcggtgc atattgccga acagattggt tatccggtgg cgctgaaatt gcgttcgccg
    91021 gatattccac ataaatcgga agttcagggc gtaatgctgt acctgcgtac agccaatgaa
    91081 gtccagcaag cggcaaacgc tattttcgat cgcgtaaaaa tggcatggcc gcaggcgcgg
    91141 atccacggcc tgttggtgca aagtatggct aaccgggctg gcgctcagga gttgcgggtt
    91201 gtggttgagc acgatccggt tttcgggccg ttgatcatgc tgggtgaggg aggtgtggag
    91261 tggcgtcctg aagatcaagc cgtcgtcgca ctgccgccgc tgaacatgaa cctggcccgc
    91321 tatctggtta ttcaggggat caaaagtaaa aagattcgtg cgcgcagtgc gctacgccca
    91381 ttggatgttg caggcttgag ccagcttctg gtgcaggttt ccaacctgat tgtcgattgc
    91441 ccggaaattc agcgtctgga tattcatcct ttgctggctt ctggcagtga atttaccgcg
    91501 ctggatgtca cgctggatat cgcgccgttt gaaggcgata acgagagtcg gctggcagtg
    91561 cgcccttatc cgcatcagct ggaagaatgg gtagaattga aaaacggtga acgctgcttg
    91621 ttccgcccga ttttgccaga agatgagcca caacttcagc aattcatttc gcgagtcacc
    91681 aaagaagatc tttattaccg ctactttagc gagatcaacg aatttactca tgaagattta
    91741 gccaacatga cgcagatcga ctacgatcgg gaaatggcgt ttgtagcggt acgacgtatt
    91801 gatcaaacgg aagagatcct cggcgtcacg cgtgcgatct ccgaccctga taacatcgat
    91861 gccgaatttg ccgtgctggt tcgctcggat ctcaaagggt taggcttagg tcgacgctta
    91921 atggaaaagt tgattaccta tacgcgagat cacggactac aacgtctgaa tggtattacg
    91981 atgccaaaca atcgtggcat ggtggcgcta gcccgcaagc tcgggtttaa cgttgatatc
    92041 cagctcgaag aggggatcgt tgggcttacg ctaaatcttg cccaacgcga ggaatcatga
    92101 gtaaggtact ggaaatgttg accacttaat cgggactggt gttattattg cccgcttatg
    92161 tcgtctgcat tgcacagagg acccttcaat gaacagagaa gaaatgcact gtgatgttgt
    92221 caaaatttaa gcgtaataaa catcaacaac accttgccca actacccaag atttctcaat
    92281 cagttgatga tgtcgatttc ttttacgctc ccgccgactt ccgggagacg ctgctggaaa
    92341 aaatagccag cgcgaagcag cgcatttgca ttgtcgccct gtatctcgaa caggatgacg
    92401 gtggcaaagg cattctgaac gcgttgtatg aagctaaaag gcagcgtccg gaactggatg
    92461 tgcgggtgct ggtcgactgg catcgtgcac aacgtggacg cattggcgct gcggcatcta
    92521 acactaacgc tgactggtac tgccgcatgg cgcaggaaaa tccgggcgta gatgttccgg
    92581 tttatggcat tccaatcaat acccgtgaag cccttggtgt tctgcacttt aaaggcttta
    92641 tcatcgacga tagcgtactt tatagcggtg ccagcctgaa cgatgtttac ctgcatcagc
    92701 acgataaata tcgctacgac cgttatcatc ttatccgtaa ccgtaagatg tcagacatta
    92761 tgtttgaatg ggttacacag aatattatga atggccgcgg cgttaatcgt ctggatgatg
    92821 ttaatcggcc aaaaagcccg gaaatcaaga acgatattcg tctgttccgc caggagctgc
    92881 gtgatgccgc ttatcatttc cagggcgatg ccgacaacga tcagctttcc gtaacgccgc
    92941 tagtggggct ggggaaatcg agtctgttga acaagaccat tttccatctt atgccttgtg
    93001 ccgagcagaa actaaccatc tgtacgccat acttcaacct gccagcaatc cttgtgcgca
    93061 atattatcca gttgctgcgc gaagggaaaa aggtcgaaat tattgttggt gataaaaccg
    93121 cgaacgactt ctacattccg gaagatgaac ctttcaagat aattggcgca ttgccttatc
    93181 tctatgagat caatctgcgt cgtttcctga gccgtttgca gtattacgtc aatactgacc
    93241 agctagtggt tcggttatgg aaagatgacg acaacaccta tcacctaaaa gggatgtggg
    93301 ttgatgataa gtggatgttg atcaccggta ataacctgaa cccgcgcgcc tggcgtctgg
    93361 atctggaaaa cgccattttg atccacgatc cgcaacttga gctggcgcca cagcgagaga
    93421 aagaactgga gctgatccgc gagcatacca ccatcgttaa gcactatcgc gatctgcaaa
    93481 gtattgccga ttatccggtg aaggttcgta aactcatccg ccgtttgcgc cgtatccgca
    93541 tcgaccgatt aattagccgc atcctgtaat cacaaccccg tcctgtacgg ggtttgtttt
    93601 ttggaggcca cgttttgcgt attctttttg tctgttcgct attgttactt tctggatgca
    93661 gccatatggc taacgatagc tggagcgggc aggataaagc tcaacacttt atcgcctcgg
    93721 cgatgctttc cgccgccgga aatgaatatt cacagcatca ggggatgagc cgggatcgca
    93781 gtgccatgtt tggattgatg ttctctgtca gtttgggggc gtcaaaagag ctttgggata
    93841 gccgccccga agggagcggc tggagctgga aggatttggc ctgggatgtc gccggtgcaa
    93901 gcaccggcta taccgtctgg caactgaccc gtcactaaag acgcatcccc ttccctttgc
    93961 gatgtagcat caaagaaacc agaaacgcca ccacggccat caaggtcaca taccagaaga
    94021 aggctgtttc cattcctatt gatttcagcg acaacgctac gtactccgcc gaaccaccaa
    94081 atatagcatt agcgaccgca tatgacaggc caacgcctaa tgcgcgaacc tgtgccggga
    94141 acatctcagc cttcagtatt ccactgattg atgtataaaa actcactatc agcagggcac
    94201 acatcaccag accaaaagcg gcataaggcg aggaaacgtt ttgcaatgct gagagaatag
    94261 gaacggtaaa aatggctgcc agcgaaccga aacataacat tgaggtacgg cgaccaatct
    94321 tatccgacag cgcgccaatg agtggttgaa taagcatgaa tacaaacaat gcggcagtca
    94381 taatgccact cgccacgttg gcatgcattc ccgcagtatt taccagatac ttctgcatat
    94441 aagtggtgaa ggtatagaaa caaagggagc ccgcagcggt aaaaccgaga accatgatga
    94501 atgcacggcg attgcgccat aatcctttca gagatccagc ttcttttaaa gcgcgcgttt
    94561 cttgttgcga agtttcatct aactgacgac gtaaccacaa cgccacaaca gctaacacag
    94621 ctcctaacgc gaaaggaata cgccatcccc actctctgag tgcagcgtct tccatggtgt
    94681 gttgtaaaac cacgacaacc agtagggcta gcagttgtcc gccgatcaac gtcacatact
    94741 gaaatgatgc gtaaaaacct ttgcgccctt caacggcaac ttcactcata taggtggcgc
    94801 tggtgccata ttctccgcca acagataatc cctgaaataa acgcgcgaga agcaataatg
    94861 ccggagccca cgtacctata gtttcataac ctgggaggca ggcgataacc agcgatccga
    94921 aacacatcat acacaccgat aacagcatcg attttttgcg accatgttta tcggctatgc
    94981 ggccaaatag ccaaccgcct attgggcgca tcaggaatcc cgcagcaaaa acacctgctg
    95041 tttgtagtag ttgagtcgtc gtgttcccgg aagggaagaa aatgtgggca aagtagagtg
    95101 aacagaacga gtagacatag aaatcgaacc actcgaccag attacctgaa gaggccccca
    95161 caatcgccca aatgcggcga cgagtatcac tacttgtcag tttgctgtct gccgttacag
    95221 tactttcaac catgccatta tgtctcctgc cgtaatccga tgcttttgtc ggtcgctttt
    95281 atttatttct tttggtaaag aaaatgttat acatttgttg cgtatcatta tgcaacctta
    95341 accatgaatt tagttagcag gaaaatggtt attgaggagc ttaaggataa atttctggta
    95401 aggaggacac gtatggaagt aggcaagttg gggaaaccgc atccgttgct gaatctggca
    95461 tatgtgggag tataagacgc gcagcgtcgc atcaggcatt tttttctgcg ccaacgcaaa
    95521 aaggccatcg tgactgatgg cctttcgc
//
LOCUS       DAPHDW010000002        76360 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.002, whole genome shotgun sequence.
ACCESSION   DAPHDW010000002 DAPHDW010000000
VERSION     DAPHDW010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 76360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 76360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..76360
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.002"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            136..1008
                     /locus_tag="RH057_000086"
     CDS             136..1008
                     /locus_tag="RH057_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417258.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
                     /protein_id="HDV3126511.1"
                     /translation="MRYFILMFTFVCSFVAAQPTIVPQLQQQVTDLTSSLNSQEKKEL
                     THKLESIFNNTQVQIAVLIVPTTKDETIEQYATRVFDNWRLGDAKRNDGILIIVAWSD
                     RTVRIQVGYGLEEKVTDALAGDIIRSNMIPAFKQQKFAQGLELAINALNNQLTSQHQY
                     PTNPSESESASSSDHYYFAIFWVFAVMFFPFWFFHQGSNFCRACKSGVYISAIYLLDL
                     FLFSDKIFSIAVFSFFFTFTIFMVFTCLCVLQKRASGKSYHSDNSGSGGGSSGGGFSG
                     GGGSSGGGGASGRW"
     gene            complement(1068..2366)
                     /gene="eno"
                     /locus_tag="RH057_000087"
     CDS             complement(1068..2366)
                     /gene="eno"
                     /locus_tag="RH057_000087"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HDV3126512.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2454..4091)
                     /gene="pyrG"
                     /locus_tag="RH057_000088"
     CDS             complement(2454..4091)
                     /gene="pyrG"
                     /locus_tag="RH057_000088"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HDV3126513.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4319..5110)
                     /gene="mazG"
                     /locus_tag="RH057_000089"
     CDS             complement(4319..5110)
                     /gene="mazG"
                     /locus_tag="RH057_000089"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HDV3126514.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     ANEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(5181..5516)
                     /gene="mazF"
                     /locus_tag="RH057_000090"
     CDS             complement(5181..5516)
                     /gene="mazF"
                     /locus_tag="RH057_000090"
                     /EC_number="3.1.27.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease MazF"
                     /protein_id="HDV3126515.1"
                     /translation="MVSRYVPDMGDLIWVDFDPTKGSEQAGHRPAVVLSPFMYNNKTG
                     MCLCVPYTTQSKGYPFEVVLSGQERDGVALADQVKSIAWRARGATKKGTVAPEELQLI
                     KAKINVLIG"
     gene            complement(5516..5764)
                     /gene="mazE"
                     /locus_tag="RH057_000091"
     CDS             complement(5516..5764)
                     /gene="mazE"
                     /locus_tag="RH057_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HDV3126516.1"
                     /translation="MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLII
                     EPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKEVW"
     gene            complement(5842..8076)
                     /locus_tag="RH057_000092"
     CDS             complement(5842..8076)
                     /locus_tag="RH057_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HDV3126517.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(8124..9425)
                     /gene="rlmD"
                     /locus_tag="RH057_000093"
     CDS             complement(8124..9425)
                     /gene="rlmD"
                     /locus_tag="RH057_000093"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HDV3126518.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKALFIPGL
                     LPQENAEVTVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHEVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILVPKLEALLPKVRACLGSLQVMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLAKQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            9482..12238
                     /gene="barA"
                     /locus_tag="RH057_000094"
     CDS             9482..12238
                     /gene="barA"
                     /locus_tag="RH057_000094"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HDV3126519.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISSVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSTQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQQ
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLVGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(12470..13810)
                     /gene="gudD"
                     /locus_tag="RH057_000095"
     CDS             complement(12470..13810)
                     /gene="gudD"
                     /locus_tag="RH057_000095"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV3126520.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDAGGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGDRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(13831..15171)
                     /locus_tag="RH057_000096"
     CDS             complement(13831..15171)
                     /locus_tag="RH057_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HDV3126521.1"
                     /translation="MTTQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKRFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAMFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPVGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(15173..16525)
                     /gene="gudP"
                     /locus_tag="RH057_000097"
     CDS             complement(15173..16525)
                     /gene="gudP"
                     /locus_tag="RH057_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HDV3126522.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPAQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQNTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16960..17409)
                     /locus_tag="RH057_000098"
     CDS             complement(16960..17409)
                     /locus_tag="RH057_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HDV3126523.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEILLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(17427..18209)
                     /gene="truC"
                     /locus_tag="RH057_000099"
     CDS             complement(17427..18209)
                     /gene="truC"
                     /locus_tag="RH057_000099"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HDV3126524.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGSQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(18209..18538)
                     /locus_tag="RH057_000100"
     CDS             complement(18209..18538)
                     /locus_tag="RH057_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HDV3126525.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWVLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(19160..19705)
                     /gene="syd"
                     /locus_tag="RH057_000101"
     CDS             complement(19160..19705)
                     /gene="syd"
                     /locus_tag="RH057_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HDV3126526.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTHLASNLAEFLNQLKPLL"
     gene            19773..20621
                     /gene="queF"
                     /locus_tag="RH057_000102"
     CDS             19773..20621
                     /gene="queF"
                     /locus_tag="RH057_000102"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HDV3126527.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSANLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKVSVALYRLDELEGQPIGHFNGTCIDDQDITIDNY
                     EFTTDYLENATCGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            20733..22097
                     /gene="ppnN"
                     /locus_tag="RH057_000103"
                     /gene_synonym="ygdH"
     CDS             20733..22097
                     /gene="ppnN"
                     /locus_tag="RH057_000103"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HDV3126528.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHNTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
     gene            22654..23943
                     /gene="sdaC"
                     /locus_tag="RH057_000104"
     CDS             22654..23943
                     /gene="sdaC"
                     /locus_tag="RH057_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HDV3126529.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
     gene            24001..25368
                     /gene="sdaB"
                     /locus_tag="RH057_000105"
     CDS             24001..25368
                     /gene="sdaB"
                     /locus_tag="RH057_000105"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HDV3126530.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     NSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            25480..26235
                     /gene="xni"
                     /locus_tag="RH057_000106"
     CDS             25480..26235
                     /gene="xni"
                     /locus_tag="RH057_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HDV3126531.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKAGRPPMPEELHDEMPALRAAFEQRGVPCWSTSGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(26290..27438)
                     /gene="fucO"
                     /locus_tag="RH057_000107"
     CDS             complement(26290..27438)
                     /gene="fucO"
                     /locus_tag="RH057_000107"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HDV3126532.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEEARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDIPALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(27466..28113)
                     /gene="fucA"
                     /locus_tag="RH057_000108"
     CDS             complement(27466..28113)
                     /gene="fucA"
                     /locus_tag="RH057_000108"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HDV3126533.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVAQALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            28660..29976
                     /gene="fucP"
                     /locus_tag="RH057_000109"
     CDS             28660..29976
                     /gene="fucP"
                     /locus_tag="RH057_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV3126534.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSGHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEIPGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            30009..31784
                     /gene="fucI"
                     /locus_tag="RH057_000110"
     CDS             30009..31784
                     /gene="fucI"
                     /locus_tag="RH057_000110"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HDV3126535.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALTWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            31893..33311
                     /gene="fucK"
                     /locus_tag="RH057_000111"
     CDS             31893..33311
                     /gene="fucK"
                     /locus_tag="RH057_000111"
                     /EC_number="2.7.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
                     /protein_id="HDV3126536.1"
                     /translation="MKQEVILVLDCGATNVRAIAVNRQGKIVARASTPNASDIAMENN
                     TWHQWSLDAILQRFADCCRQINSELTECHIRGIAVTTFGVDGALVDKQGNLLYPIISW
                     KCPRTAAVMDNIEQLISAQRLQAISGVGAFSFNTLYKLVWLKENHPQLLERAHAWLFI
                     SSLINHRLTGEFTTDITMAGTSQMLDIQQRDFSPQILQATGIPRRLFPRLVEAGEQIG
                     TLQNSAAAMLGLPVGIPVISAGHDTQFALFGAGADQNEPVLSSGTWEILMVRSAQVDT
                     SLLSQYAGSTCELDSQAGLYNPGMQWLASGVLEWVRKLFWTAETPWQMLIEEARLIAP
                     GADGVKMQCDLLSCQNAGWQGVTLNTTRGHFYRAALEGLTAQLQRNLQMLEKIGHFKA
                     SELLLVGGGSRNTLWNQIKANMLDIPVKVLDDAETTVAGAALFGWYGVGEFNSPEEAR
                     AQIHYQYRYFYPQTEPEFIEEV"
     gene            33313..33735
                     /gene="fucU"
                     /locus_tag="RH057_000112"
     CDS             33313..33735
                     /gene="fucU"
                     /locus_tag="RH057_000112"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HDV3126537.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMGPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            33793..34524
                     /gene="fucR"
                     /locus_tag="RH057_000113"
     CDS             33793..34524
                     /gene="fucR"
                     /locus_tag="RH057_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HDV3126538.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(34568..35668)
                     /gene="rlmM"
                     /locus_tag="RH057_000114"
     CDS             complement(34568..35668)
                     /gene="rlmM"
                     /locus_tag="RH057_000114"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HDV3126539.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPADAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(35661..36056)
                     /locus_tag="RH057_000115"
     CDS             complement(35661..36056)
                     /locus_tag="RH057_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HDV3126540.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            complement(36075..36992)
                     /gene="gcvA"
                     /locus_tag="RH057_000116"
     CDS             complement(36075..36992)
                     /gene="gcvA"
                     /locus_tag="RH057_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HDV3126541.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            complement(37343..37570)
                     /locus_tag="RH057_000117"
     CDS             complement(37343..37570)
                     /locus_tag="RH057_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HDV3126542.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDTGMISYKDANGNKQQINRTDVKEMVELDQ"
     gene            37762..38967
                     /gene="csdA"
                     /locus_tag="RH057_000118"
     CDS             37762..38967
                     /gene="csdA"
                     /locus_tag="RH057_000118"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HDV3126543.1"
                     /translation="MNVFNPAQFRAQFPALLDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTGAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGAVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            38967..39410
                     /gene="csdE"
                     /locus_tag="RH057_000119"
     CDS             38967..39410
                     /gene="csdE"
                     /locus_tag="RH057_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HDV3126544.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVTENGKMHFFGDSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAAAKQV"
     gene            complement(39461..40267)
                     /gene="tcdA"
                     /locus_tag="RH057_000120"
     CDS             complement(39461..40267)
                     /gene="tcdA"
                     /locus_tag="RH057_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HDV3126545.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSVGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(40344..41441)
                     /gene="mltA"
                     /locus_tag="RH057_000121"
     CDS             complement(40344..41441)
                     /gene="mltA"
                     /locus_tag="RH057_000121"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HDV3126546.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LSDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            41650..41726
                     /locus_tag="RH057_000122"
     tRNA            41650..41726
                     /locus_tag="RH057_000122"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:41684..41686,aa:Met,seq:cat)
     gene            complement(41801..43054)
                     /gene="amiC"
                     /locus_tag="RH057_000123"
     CDS             complement(41801..43054)
                     /gene="amiC"
                     /locus_tag="RH057_000123"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HDV3126547.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEKEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            43286..44617
                     /gene="argA"
                     /locus_tag="RH057_000124"
     CDS             43286..44617
                     /gene="argA"
                     /locus_tag="RH057_000124"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HDV3126548.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     IHRQLDSGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERITAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(44679..46505)
                     /gene="recD"
                     /locus_tag="RH057_000125"
     CDS             complement(44679..46505)
                     /gene="recD"
                     /locus_tag="RH057_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HDV3126549.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASHPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHAIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERAGQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(46505..50047)
                     /gene="recB"
                     /locus_tag="RH057_000126"
     CDS             complement(46505..50047)
                     /gene="recB"
                     /locus_tag="RH057_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HDV3126550.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLTVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIALVVFETWKGPQALLRDINRYLQGEAPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIEQLLAEPLSIRDLVITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDAFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDVVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYNYDRHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(50040..52928)
                     /gene="ptrA"
                     /locus_tag="RH057_000127"
     CDS             complement(50040..52928)
                     /gene="ptrA"
                     /locus_tag="RH057_000127"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HDV3126551.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISEQTFADWQQKAANIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFSSEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMVNANGYTQRLPQLFQALLEGY
                     FSYTATEDQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(53104..56472)
                     /gene="recC"
                     /locus_tag="RH057_000128"
     CDS             complement(53104..56472)
                     /gene="recC"
                     /locus_tag="RH057_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HDV3126552.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LDREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPEWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNPLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEDPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPTTGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FRTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(56485..56808)
                     /locus_tag="RH057_000129"
     CDS             complement(56485..56808)
                     /locus_tag="RH057_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HDV3126553.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVMIVTALSGIQRTLMNSLASR
                     NQYQQLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGGREGEMTRLH
                     CPNRQ"
     gene            complement(56793..57200)
                     /locus_tag="RH057_000130"
     CDS             complement(56793..57200)
                     /locus_tag="RH057_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HDV3126554.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDARVCLRLLADNEALLVAGYEGVSL
                     WRTGEVIDGNIVFSPRGWSDFCPLKERALCQLP"
     gene            complement(57197..57760)
                     /locus_tag="RH057_000131"
     CDS             complement(57197..57760)
                     /locus_tag="RH057_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV3126555.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(57751..58221)
                     /locus_tag="RH057_000132"
     CDS             complement(57751..58221)
                     /locus_tag="RH057_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HDV3126556.1"
                     /translation="MKTQRGYTLIETLVAMLILVMLSASGLYGWQYWQQSQRLWQTAS
                     QARDYLLYLREDANWHNRDHSISVIREGTLWCLVSSAAGANTCHGSSPLVFVPRWPEV
                     EMSDLTPSLAFFGLRNTAWAGHIRFKNSTGEWWLVVSPWGRLRLCQQGETEGCL"
     gene            complement(58406..59200)
                     /gene="thyA"
                     /locus_tag="RH057_000133"
     CDS             complement(58406..59200)
                     /gene="thyA"
                     /locus_tag="RH057_000133"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HDV3126557.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGIKAPVAI"
     gene            complement(59207..60082)
                     /gene="lgt"
                     /locus_tag="RH057_000134"
     CDS             complement(59207..60082)
                     /gene="lgt"
                     /locus_tag="RH057_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HDV3126558.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(60233..62479)
                     /gene="ptsP"
                     /locus_tag="RH057_000135"
     CDS             complement(60233..62479)
                     /gene="ptsP"
                     /locus_tag="RH057_000135"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV3126559.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSRIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDYAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(62492..63022)
                     /gene="rppH"
                     /locus_tag="RH057_000136"
     CDS             complement(62492..63022)
                     /gene="rppH"
                     /locus_tag="RH057_000136"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HDV3126560.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            63707..64396
                     /gene="mutH"
                     /locus_tag="RH057_000137"
     CDS             63707..64396
                     /gene="mutH"
                     /locus_tag="RH057_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HDV3126561.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALAGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVAPLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            64465..65178
                     /locus_tag="RH057_000138"
     CDS             64465..65178
                     /locus_tag="RH057_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV3126562.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASIAWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            65316..65534
                     /gene="ygdR"
                     /locus_tag="RH057_000139"
     CDS             65316..65534
                     /gene="ygdR"
                     /locus_tag="RH057_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HDV3126563.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            65642..66682
                     /locus_tag="RH057_000140"
     CDS             65642..66682
                     /locus_tag="RH057_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HDV3126564.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(66714..67907)
                     /gene="lplT"
                     /locus_tag="RH057_000141"
     CDS             complement(66714..67907)
                     /gene="lplT"
                     /locus_tag="RH057_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HDV3126565.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILFAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASTIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAAARPGQSWNLINMTRSFLNACTSLWRNGET
                     RFSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKL
                     VTLETVSRCMPAGILIGVVVLIFSLQHELLPAYALLMLIGVMGGFFVVPLNALLQERG
                     KKSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMIGIPVVPIGIGFGALFALAITALWI
                     WQRRH"
     gene            complement(67900..70059)
                     /gene="aas"
                     /locus_tag="RH057_000142"
     CDS             complement(67900..70059)
                     /gene="aas"
                     /locus_tag="RH057_000142"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HDV3126566.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVEMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPEL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVQEVPTAENVRGEMERGWYDTGDIVRFDELGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            70645..71676
                     /gene="galR"
                     /locus_tag="RH057_000143"
     CDS             70645..71676
                     /gene="galR"
                     /locus_tag="RH057_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HDV3126567.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIAANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(71683..72945)
                     /gene="lysA"
                     /locus_tag="RH057_000144"
     CDS             complement(71683..72945)
                     /gene="lysA"
                     /locus_tag="RH057_000144"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HDV3126568.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFDVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDMLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSVPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            73067..74002
                     /gene="lysR"
                     /locus_tag="RH057_000145"
     CDS             73067..74002
                     /gene="lysR"
                     /locus_tag="RH057_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HDV3126569.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPAGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGVSVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSEHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(73989..74681)
                     /gene="ygeA"
                     /locus_tag="RH057_000146"
     CDS             complement(73989..74681)
                     /gene="ygeA"
                     /locus_tag="RH057_000146"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HDV3126570.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQVLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLMEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(74810..76228)
                     /gene="araE"
                     /locus_tag="RH057_000147"
     CDS             complement(74810..76228)
                     /gene="araE"
                     /locus_tag="RH057_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HDV3126571.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGATFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
BASE COUNT        18677 a        20563 c        18653 g        18467 t
ORIGIN      
        1 aaaatggatt taatcatgtc aaaacattca ccaaaattat atttattttt tctgtgttag
       61 tttttaatga taacgaatct aaaattaccg atgccgccgt caatttattt atccagattt
      121 aatgagaaat ttataatgcg ctatttcata ctaatgttca ctttcgtgtg ttcctttgtt
      181 gcagcccaac caactattgt cccacaatta cagcaacaag ttacggatct tacgagtagc
      241 ttaaactcac aagaaaagaa agaactgacg cacaagttag aatctatttt caataatacc
      301 caagtgcaaa ttgctgtatt aattgttccc acaaccaaag acgagacaat tgaacaatat
      361 gctacaagag tttttgacaa ttggcgttta ggagatgcca aacgtaatga tgggatactg
      421 atcattgttg cctggtcaga tcgtactgtc cgcatccagg taggttatgg gctggaagaa
      481 aaggtaaccg atgctctggc tggagatatc atccgtagca acatgatacc cgcatttaaa
      541 caacaaaaat ttgctcaggg attagagcta gctataaacg ctttgaataa ccaactcact
      601 tcacaacacc aatatccgac taatccttca gaaagtgaat cagcgtcttc cagtgatcat
      661 tattactttg ctattttttg ggtatttgca gtgatgttct tccctttctg gttttttcat
      721 caaggcagta atttttgtcg cgcatgtaaa agtggagtct atatttcagc aatctatctt
      781 ttagatttat tcctgttctc cgataaaatt ttttccattg ctgtattttc cttctttttt
      841 actttcacca tatttatggt ctttacctgt ttatgtgtac ttcagaaaag agcatcaggt
      901 aaaagttatc attcagacaa cagcggttcc ggtggaggct caagtggcgg cggatttagc
      961 ggcggtggcg gttcttctgg aggtggcggc gcatccggcc gctggtaact caaccataaa
     1021 aaatgccagc ccggaggctg gcatttttaa atcagataaa gtcagtctta tgcctggcct
     1081 ttgatctctt tacgaccgtt gtacggtgct ttttcgccca gagcttcttc gatacgaatc
     1141 agctggttgt atttagcaac acggtcagaa cggctcatag aaccagtttt gatctggcct
     1201 gcagcagtac caacagccag gtcagcgatg gtagcgtctt cagtttcgcc agaacggtga
     1261 gagataactg cagtgtagcc agcatctttc gccatcttga ttgcagccag agtttcggtc
     1321 agagaaccga tctggttgaa tttgatcagg atggagttag cgataccttt ttcgatacct
     1381 tctttcagga tcttggtgtt ggttacgaac aggtcgtcac caaccagctg gattttgtcg
     1441 cccagaactt tggtctggta tgcgaaaccg tcccagtcag attcgtccag accgtcttcg
     1501 atagaaacga tcgggtactg tttggtcagt tcttccagga agtgagtgaa ttcttcagag
     1561 gtgaacgctt tgttgccttc gccagccaga acgtatttac catctttgta gaattcagaa
     1621 gctgcgcagt ccatcgccag agtgatgtct ttgcccagtt cataaccagc agctttaaca
     1681 gcttcagcga taacagccag agcttcagcg ttggaaccca ggttcggcgc atagccacct
     1741 tcgtcaccaa cagcagtgtt catgcctttc gctttcagaa cttttgccag gtgatggaaa
     1801 acttcagaac ccatgcggat ggcttctttc acagttttcg cgccaaccgg ctgaatcatg
     1861 aattcctgga tatcaacgtt gttgtcagcg tgctcaccac cgttgatgat gttcatcatc
     1921 ggaaccggca tagagtattt gcccggagta ccgttcagtt cagcgatgtg ctcgtacagc
     1981 ggcatacctt tagcagctgc agcagctttg gcgttagcca gagatacagc caggattgcg
     2041 ttcgcgccga atttggattt gttttcggtg ccgtccaggt cgatcatgat cttgtcaatg
     2101 ccagcctgat ctttagcatc tttgccaatc agcgcctgag cgatcgggcc gtttaccgca
     2161 gcaacagctt tggttacgcc tttacccagg aaacgggatt tgtcgccatc gcgcagttcc
     2221 agagcttcac gggaaccagt agaagcacct gacggagcag ctgccatacc gacgaaacca
     2281 ccctccagat gtacttcggc ttcaacagtc gggttaccac gggagtcgat gatttcacga
     2341 ccgatgattt ttacgatttt ggacattagg ttttcctcaa gtcactagtt aaactgaaac
     2401 tccagacaaa caacgcgtac ccagggtacg cgttgccgtt ctaacttttt tacttacttc
     2461 gcctgacgtt tctggaactc gctggcggct ttcacaaagc ctgcaaacag cgggtgacca
     2521 tcacgtggag tagaagtaaa ctccggatgg aactggcaag ccacgaacca cgggtgattc
     2581 ggaacttcga tgatctcgac caactgatca tccccggaac ggcccgcaac gcgcagacct
     2641 gcatcttcaa tctgtttcaa cagcatgttg ttgacttcgt aacggtgacg atgacgctca
     2701 acaattgtcg gcgcattgta cagctggcga accaggctat cgtcaaccaa ctggcactgc
     2761 tgtgcgccga gacgcatggt accgccgaga tcgctcttct cgctacgaac ttcaacgttg
     2821 ccgttttcat cgcgccactc ggtaatcagc gccacaaccg ggtacttaca gtctggcaca
     2881 aattccgtag agttggcgtt ctccatgttg gcaacatggc gagcgtaatc aattaacgcc
     2941 acctgcatac ccaggcaaat gcccagataa ggaatattgt tctcacgcgc aaaacgcgcg
     3001 gtcgtaatca tgccttctac gccacgatag ccaaaaccgc caggtactag gattgcgtcc
     3061 agacctttaa ggatttcaac gccgcgcgtt tcaacatctt gtgaatcgat cagtttgatg
     3121 ttgacgctga cacgattctt cagcccaccg tgtttcagtg cttcgatcac tgatttataa
     3181 gcatccggca gttcaatgta cttgccgacc ataccgatgg tgacttcact taccgggttc
     3241 gcttcttcga agataacctg ttcccattcg gacagattcg cttccgggca gtttaagctg
     3301 aatcgtttac aaatataatc gtccagcccc tgagatttca acaggcccgg aattttatag
     3361 atggaatcga cgtctttcag agaaataacc gctttttccg gaacattaca gaacaatgca
     3421 atcttcgcac gttcgttcgc cggaacagcg cgatctgaac gacaaatcag gatgtcaggc
     3481 tggataccga tggagagcag ctcttttaca gagtgctgag tcggtttggt tttgacttca
     3541 ccagacgctg ccatgtacgg caccagcgtc aggtgcataa acagagtgtg ctcacggcca
     3601 atttcaacag ccatctggcg aatcgcttcg aggaacggca aggattcgat atcacctact
     3661 gtaccgccga tttctaccag tactacgtca tgaccttcgc caccttccag cacgcgctct
     3721 ttgattgcgt tagtgatgtg cggaataacc tgcacggttg cgccgaggta gtcaccgcgg
     3781 cgttctttac gcagaacgtc agagtagata cgacccgtgg tgaagttgtt gcggcggctc
     3841 attttggtac gaatgaaacg ctcgtagtgc cccaggtcca ggtcggtttc agcgccgtct
     3901 tcagtaacga acacttcccc gtgttggatt gggctcatag tacctggatc gacgttgatg
     3961 tacggatcca gtttcatgat ggtcacattg aggccacggg cttcaagaat ggctgcgagg
     4021 gaggctgcgg caatgccttt acccagagag gatacgaccc cgccggtcac aaaaatatag
     4081 ttcgttgtca tgctgaacct gagaagttag gttgaaaaga cgatggaata accaggacgg
     4141 gaaagcagta tacccgaaca tgacctgtgc cacaaacttt cattatccct cctcttcgcc
     4201 agcgcactat tgaaatcagg agtgagaaaa tagccccttt ggggtaaatg tttttgacgc
     4261 aaatcaagcg cttgtcattt aaaaaatgac acaaatggcg cttgaccgcg taattccctt
     4321 agagatcaat ttcctgccgt tttacctgtt gccagacttc ttccattgtt tcgaggtcaa
     4381 cacctgtcat ttccagtcca cgcgcggcaa caatacgctc cacttcgcga aaacgacgct
     4441 cgaatttttc gttcgctttt tgcaatgcga tttctgcttt cgtccctaaa tggcgagcca
     4501 gattaaccgt ggcaaacagc aggtccccca tttcctcctc cagtttagcc tggtcgacaa
     4561 cagcctgccg cgcttcgtac atcacctcgt cgatctcttc gtagacttta tcgactaccg
     4621 gaccaagcgt cgtccaatcg aagccaacgt tggcgcaacg tttctggatt ttttgcgcac
     4681 gcattaaagc cggtaaacta cgaggaatat cgtccagcgc cgaatgctgc gctttctgcg
     4741 cgcgctcttc ggttttgatt tgctcccaac gggcaagcac ttcactactg ttttcggcag
     4801 aactatcagc aaaaacatgc ggatggcgac gctctaattt atcgctaata gcagcgcaaa
     4861 tatcattaaa gtcaaagcgc ccttcttcct gagccatttg cgcgtaaaac accacctgga
     4921 atagcagatc gcccagttca ccgcgaagat cgtcaaaatc ttcacgggcg atggcgtcca
     4981 gcacttcgta ggtttcttca agggtgtaag gcgcaatggt ggcaaatgtc tgctctttat
     5041 cccacgggca gccgttttcc ggatcgcgca ggcgctgcat aatagtgagc aaacggtcga
     5101 tttgattcat tgaattgtcc tgaaaattgc gggtctgtca ggtggaaacc tgtgaccaga
     5161 atagaagtga gttagtaaca ctacccaatc agtacgttaa ttttggcttt aatgagttgt
     5221 aattcctctg gggcaactgt tcctttcttc gttgctcctc ttgcccgcca ggcgatactt
     5281 tttacctgat cagctaacgc tacgccatca cgttcctgac cggataaaac aacttcgaac
     5341 ggatatcctt ttgattgcgt tgtataagga acacacagac acatacctgt tttgttgttg
     5401 tacatgaaag gactcaggac aacagctgga cgatgtccag cttgctcgct accttttgtc
     5461 gggtcaaaat caacccaaat cagatcgccc atatcgggta cgtatcggct taccattacc
     5521 agacttcctt atctttcggc tctccccagt cgatattctc gtggaggttt tccggcgtga
     5581 tgtcgttgac cagttcagca agcgtaaata cgggctcttt acgcactggc tcaataatta
     5641 atttgccatc caccaggtca atcttcactt catcatcaat attcagattg agcgcctgca
     5701 ttaacgtagc cgggatccgc accgccggtg aatttcccca acgctttacg ctactgtgga
     5761 tcataaccct ttcctcaaac cgctatcata tgtagataca gtatatatca atctacattg
     5821 tagatacgag caaatttcgg cctaactccc gtgcaaccga cgcgcgtcga taacatccgg
     5881 cacctggttg agtttaccca gcacgcgccc cagcacttgc aggttgtaaa tctcaatggt
     5941 catgtcgatg gtcgccagtt gctgtttggt gtcgctacgg ctggcaacgc caagcacgtt
     6001 caccttctcg ttggcgagaa tggtcgtgat atcacgtaac aacccactac gatcattagc
     6061 caccacgcgg accaccagcg aatatccggc ggagtagctc tcaccccata ccgcgtcaac
     6121 aatgcgttct ggcgcatggg agcgcagttc cgccagttgt tcgcaatcgg cgcggtgtac
     6181 tgaaataccg cgcccctggg taatgaagcc gacaatctca tctccaggaa tcggctggca
     6241 gcagcgcgcg atgtggtgca tcaggttgcc aacaccttcg accaccacgc gaccgttatc
     6301 tttactgcgg ttttgcggcg tgtagctttt ttgctgaagt tgcttcagcg cggcggcgtc
     6361 ctgctcttcg gcactcggct tattaaattg cgattgcagg aagttcacca tctgattgag
     6421 acggatatcc ccgccaccaa tcgccgccag caactcgtcg acatcattaa agttgtaacg
     6481 cggcagcaga tgtttttctg cttctttcag gctgatcccc agatgttcca gctcgtcgtc
     6541 aaggatttgc cgcccagcca gaatgttttt gtcacggtcc tgtttacgga accaggcgtg
     6601 aattttcgaa cgcccacggc tggttgtgac gtaaccgagg tttgggttta accagtcacg
     6661 gctggggttc ggctgtttct gggtgataat ttcaatctgg tcgcccatct gcagctggta
     6721 ggtgaacggc acaatgcgcc cgccaatttt tgccccgatg cagcggtgtc cgacatcact
     6781 gtggatgtgg taagcgaagt ccagcggcgt tgatcccgca ggcaaatcaa cgacatcacc
     6841 tttcggcgta aagacataca cccggtcgtc aaagacctgg ctgcgtactt cgtcgagcat
     6901 ttcgccggaa tcagccatct cttcctgcca cgcaatcagt ttacgcagcc aggcaatccg
     6961 gtcttcatgt cccgaacgtg cgccgccagc tgccgcgccc tctttatatt tccagtgcgc
     7021 agcaacaccc aactctgcat cttcatgcat ctgtttggtg cggatttgga tctcaacggt
     7081 tttgccacct ggccccagaa ccacggtatg aatagactga taaccgtttg gtttcgggtt
     7141 agcgacataa tcgtcaaact catccggcag gtggcgatag tgagtgtgca ctatccccag
     7201 tgcggcataa caatcctgta aacgctcggc gacaatacgt accgcacgca catcaaacag
     7261 ctcatcgaag gcgaggttct ttttctgcat tttgcgccag atactgtaga tgtgtttcgg
     7321 acgaccatac acttcagctt taacgccttc agctttcatc tcagcgcgca gatggccgac
     7381 aaactcttcg atatagtgtt cgcggtcgag acggcgttca tgcagcagtt ttgcgatgcg
     7441 tttgtattcg gtcgggtgga gataacggaa gcagtaatct tccagctccc atttcagttg
     7501 cccaatccca agacggttcg ccaacggcgc gtagatattg gtgcactctt ttgccgccag
     7561 tacgcgttca tcttccggcg catcttttac ttcgcgcaga tgagcaatac gctccgccag
     7621 tttgatgacc acgcagcgaa aatcatcgac catcgccaat aacatccggc gaacgttatc
     7681 gacctgttcg gaggaaacag aatcagtgtg cgtcgctttc agctggcgga tcgccgccat
     7741 atcacgcacg ccgtgaataa ggttaacgac cgacttaccg acgctctcac gcagcacatc
     7801 ttcgctgact acgttggcat cagccagagg gaacagcagc gccgcccgca gcgtgtcaat
     7861 gtccatactt aatgtcgaga ggatctccac catctcaaca ccacgccaca ataacagact
     7921 ggcatccgga tgcccctgcg tctgttgcag acaatacgcc caggtttcgg ctaagcactc
     7981 acacgacttc tggctggtaa tacccagact tgcgatccat ttttccggat caaattcacc
     8041 agccttattg atatgtgcac ttcttaccgc aaccattgtc ctctccttta gggaccagac
     8101 ctgccgaaat cggcaaatcg taactattta acgcgcgaga aaagtaccat cgattccaga
     8161 tgtcccgtgt gtgggaacat atccagcatc gccagtcgcg caatggtata tcctgctttt
     8221 aataacgctt cgctatcccg agccagcgtt gcagggttac aggatacata aactatacga
     8281 ataggttcca gttttataat ttgctgcata acacctgcgg cacctgctcg cgccgggtcc
     8341 agcaacactt tatcgaagcc gtttttcgcc cacggctgct ttgtgacatc ttcttcaaga
     8401 ttttcgtgat aaaacgtcac attctgtaaa ccattaagac gcgcattctg ctggcctttt
     8461 tccaccagcg ccggaacacc ttctacaccg accacactgg ctgcttgttt cgccaatggc
     8521 agtgtaaagt tgcccatacc gcagaacaga tccagtacgc gatcttcagg ttgcacatcc
     8581 agccattcca acgcacgcgc taccattttt tggttcacac ccgcattgac ctgaataaaa
     8641 tcgcgcgggc taaaagttaa gcgcaacccg tttgagtcat accagggcat ctcaccagag
     8701 acggtttcga gtatctcact atcgggggcg agatacagat ccaggccttc agaatgcgaa
     8761 aagcgttcca gtttttcgcg atctgccgaa cttagcggtg cggtatggcg caaaatcatc
     8821 agcgtgccgc tggttgcctg taccagttca acatgaccaa gatggcgcat aacttgtaag
     8881 ctgcccaggc atgccctgac tttgggcagc aatgcttcaa gtttgggcac taaaatgggg
     8941 cattgtttaa cgtcgacaat gtcactggag cccgctttgc gaaagcccat ctgaagttgc
     9001 tgtgttttcg gtaagtagtt caaacttaaa cgcgcgcgac ggcgatagcc ccagggaaca
     9061 tcggcgatca cttcagagac ttcgtgtttc attaatcggg cgagtgccgc acttttgctt
     9121 cgctgctgta aatccacgct ggcgtgttgt tgctgacagc caccgcatac gccaaaatga
     9181 gggcagcgtg gcgtttcgcg ttccgggcta tcgcttaacc ggcgcacgac tttagcgcgg
     9241 gcgtactgtt ttttatcttc agtaacagta acttccgcgt tttcctgtgg cagtaatcca
     9301 ggaataaata acgctttgcc gttatgtcgc gccacgccct gaccaaaaga gtcgaggtcg
     9361 ttgactgaaa cggttatgat ctgacgcgtc gtcgtgcgtc gttttgcaga gtagaattgc
     9421 gccattggcg agactttctc aatttaacag tgtgacctta attgtcccat aacggaactc
     9481 catgaccaac tacagcctgc gcgcacgcat gatgattctg atcctggcac cgaccgtcct
     9541 tattggttta ttgctgagta tctttttcgt cgtgcatcgc tataacgact tgcagcgtca
     9601 actggaagat gccggtgcca gcattattga gccgcttgca gtttctactg aatatggcat
     9661 gagcctgcaa aatcgcgaat ctatcggtca gttaataagc gtactgcatc gtcgccattc
     9721 cgatattgtt cgcgcgattt cggtttatga tgaaaataac cgactctttg tcacctccaa
     9781 ttttcatctt gatccctcat caatgcagct cggcagcaac gtgccgtttc ctcgccagct
     9841 cactgtcact cgtgacggcg atattatgat cctccgcacg ccgattattt ctgagagtta
     9901 ctcccccgac gaatcgccca gtagcgatgc caaaaatagt caaaatatgt tgggatatat
     9961 tgcgcttgag ctggatctta aatcggttcg cttgcagcaa tataaagaga tctttatttc
    10021 cagcgtgatg atgctgtttt gtatcggtat tgcgcttatt tttggctggc gcttaatgcg
    10081 cgatgtaacc ggtccgattc gcaacatggt gaataccgtc gaccgcatcc gtcgcgggca
    10141 actcgacagc cgggtggaag gatttatgct cggcgagctg gatatgctga aaaacggtat
    10201 caactcgatg gcaatgtcgc tggctgctta tcacgaagag atgcagcaca atatcgacca
    10261 ggcgacgtcc gatctgcgtg aaacgctgga gcagatggaa attcagaacg ttgagttgga
    10321 tctggcgaaa aagcgcgccc aggaagcggc gcgtattaaa tccgagtttc tggcaaatat
    10381 gtcacacgag ctgcgtacac cactgaatgg tgttattggc tttacccgcc tgacgctgaa
    10441 aacagaatta acaccaacgc agcgcgatca cctgaatacg attgaacgtt cggcaaataa
    10501 tttgctggca attattaatg atgttctcga cttctcgaaa ctggaagcag gtaagctgat
    10561 tctggaaagt attccattcc cactacgcag cacgctggat gaagtcgtta ctctgctggc
    10621 acattcttct cacgataaag ggttggaact gacgctcaat attaaaagcg acgtgcctga
    10681 taacgtgatc ggcgacccgc tgcgattaca gcaaatcatc actaacctgg tggggaatgc
    10741 aattaaattc accgagaatg gcaacattga tattctggta gaaaaacgtg cgctgagtaa
    10801 taccaaagtg cagattgaag tgcagattcg ggataccggc attggtattc ctgaacgcga
    10861 tcaatcgcgc ttattccagg ccttccgaca ggctgatgcc agtatttccc gccgtcatgg
    10921 tggcaccggg ctggggctgg tgattacaca aaaactggtt aatgaaatgg gcggcgatat
    10981 ttcgttccat agccagccga atcgcggttc aactttctgg ttccacatta atctcgatct
    11041 gaacccgaac attattatcg aagggccatc cacccagtgc ctcgcaggta agcgcctggc
    11101 ctatgtcgaa ccaaactccg cagcagcgca atgcacgctg gatattttaa gtgaaacgcc
    11161 gctggaagtg gtttatagcc caacgttctc cgcgctgcct cccgcgcatt acgacatgat
    11221 gttgttaggc atcgcggtga ccttccgcga gccgctaaca atgcaacatg agcgattagc
    11281 gaaagcggta tcgatgaccg atttcctgat gctggcactt ccttgccatg cacaagtcaa
    11341 tgctgaaaaa ctcaagcaag atggtatcgg cgcgtgtctg ctgaaaccat taacacctac
    11401 acgcctgttg cctgccctga cggaattttg tcatcacaaa caaaacacgc ttttgcctgt
    11461 aaccgatgaa agtaagctgg caatgacagt catggcggtt gatgacaacc ccgctaacct
    11521 gaaacttatc ggcgcattgc tggaagatat ggtgcaacat gtggaacttt gcgatagcgg
    11581 gcatcaggcg gttgaacggg cgaaacagat gccgttcgat ttgatcttaa tggatattca
    11641 aatgcctgac atggatggca ttcgggcctg cgagctcatc caccagctcc cccatcagca
    11701 acaaacgccg gttatcgcgg taacggcgca tgcaatggcc gggcaaaaag agaagctgct
    11761 tggcgcaggg atgagcgatt atctggcgaa accgattgaa gaagagcgat tgcataattt
    11821 gttgttgcgc tacaagcctg gcagcggtat ttcctctcgc gtcgtgacgc ccgaagtcaa
    11881 cgaaattgtg gtgaacccga atgcgaccct cgactggcaa ctggcactac gccaggcagc
    11941 aggaaaaacc gatttagcgc gcgatatgct gcaaatgtta ctcgatttcc tgcctgaagt
    12001 tcgcaacaaa gttgaggaac agctggttgg agaaaacccg gaaggcctgg ttgatttgat
    12061 tcataaactg catggcagtt gcggctatag cggtgtgccg cgtatgaaga atctctgcca
    12121 actgatcgaa caacagctac gtagcggtac taaagaagaa gatttggagc cggagctgct
    12181 ggaactgttg gacgagatgg ataatgtagc gcgcgaagcc agcaaaattc tcgggtaacg
    12241 gacgtcttgt cgagtagcaa tcggcttttt atgacgctgg tttcagattg gtgacaaagt
    12301 gcctgtgttt atgccggatg cggcgttaac gccttatccg gcctacaaaa ccaattaaat
    12361 tcaatgaatt aaaaaatgat gtagacctga tacatagcac aacaggctat tttgcgttta
    12421 gcatcagtct caagccggct ccagatagag ccggttttgg ttttctgtct taacgcacca
    12481 tgcacgggcg cttgttatcg aacgtccagc caggaatcag atactgcatt cccatcgcat
    12541 cgtcacgcgc gccaagcccg tgtttctgat acagctcatg ggctttcatc acttgatcca
    12601 tatcgatttc tacacccagc cccggttttt ctggcacctg taccagcccg cctttgatct
    12661 caaacggttc tttggtcagg cgctgattac cttcctgcca gatccagtgc gtatcaatag
    12721 cagtaatttt acccggtgca gcggcggcaa catgggtaaa catcgccagg gaaatatcga
    12781 agtggttgtt tgagtgtgaa ccccaggtca ggccaaattc atggcacatt tgcgccacac
    12841 gtaccgaacc ttgcatcgtc cagaaatgcg gatccgccag cgggatatca acggattgca
    12901 gggagagcgt atggcccatc tgccgccagt cggtggcgat catattggtt gcggtcggca
    12961 ggccagttgc gcgacggaac tctgccatca cttcacgccc ggagaaacct tgctccgcac
    13021 cacacggatc ttctgcataa gccagcgacc ctttcaggta tttaccaatt ttaatcgctt
    13081 cgttcagcga ccaggcaccg ttaggatcga gcgtaatacg cgcctgcggg aagcgttgcg
    13141 ccagtgccac aatagactcg gcctcttctt ccccggccag tacaccgcct ttcagtttga
    13201 aatcgttgaa gccatatttt tcatacgccg cttccgccag gcgcaccacc gcatccggcg
    13261 tcatcgcttc ttcatgacgc aggcgatacc agtcgcatga gtcatccggc tggctttgat
    13321 acggcagcgg tgtggctttg cgatcaccga cgaagaacag ataaccgagc atttcgactt
    13381 cgctacgctg ttgaccatcg cccagcaggg atgccacgtt tacccccaga tgctgcccca
    13441 gcagatccag cattgccgct tctatcccgg taactacatg aatagtggta cgcaaatcaa
    13501 atgtctgcaa accgcgccca ccagcatcac gatcggcaaa agtattacgc accagcgtca
    13561 gaacgttttt gtattcaccc agcgttttac ctaccaccag cggaatcgca tcttccagcg
    13621 ttttacggat tttctcgccg ccgggaattt cccctacgcc agtgtgacca gaattatctt
    13681 tgataatcac aatattacgc gtaaagaacg gtgcgtgtgc accactcaga ttcatcagca
    13741 tactgtcatg acccgccacc gggatgacct gcatttcagt aacaacaggc gtcgtaaatt
    13801 gagaactcat atttatgtcc tttttcagaa tcaatgacgg ccgaaaacgg gacgtttacg
    13861 gtcaaaggtc cagccgggga tcaggtactg catcggacct gcgtcgttac gcgcaccgac
    13921 aggcagacgt ttataggcct catgtgcctt ttgtacctgt tcccagtcca gttccacgcc
    13981 cagaccgggc gcatcaggaa cggcaatttt tccgttttta atctccagcg gatttttggt
    14041 caggcgacaa tcgccctcct gccaaatcca gtgggtatcg atagcggtag gattacccgg
    14101 tgccgccgcg cccacatggg taaacatcgc cagagagata tcgaaatggt tattagaatg
    14161 gcagccccag gtcagccccc agtcgtcgca aagctgcgcc acacggactg caccggaaag
    14221 cgtccagaag tgcggatcgg caagtggaat atctaccgca ttgagcatca ccgcatgacc
    14281 catttcgcgc cagttggtgg cgatcatgtt agtcgcgacg ggcaagccgg tcgcccgccg
    14341 aaactccgcc ataacttcac gaccggagaa accctgttct gcgccgcatg gatcttcggc
    14401 ataggtaaga acatcattca gccctttgca tagagaaatg gcttcatcaa gtagccaggc
    14461 accgttggga tcaacggtaa tccgcgcatc agggaagcgt ttcttcaatg cacgaacagt
    14521 gtcgatttct tgctcgccag gtaacacccc gcccttaagt ttgaaatctt taaagccata
    14581 gcgatcctgt gaggcttccg ccagacgcac aacggcttcg ctgttcatcg ctttctgatg
    14641 gcgcaactga taccactcat ggttgcccgg cgtattttcc agataaggaa gatcggtttt
    14701 ggtccgatca ccgatataaa acagataacc gaggacggta atagtctcgc gttgcttgcc
    14761 tggccccaac agttcgcaga ccggaacatt cagcgcctta cctagcaggt caagcaaggc
    14821 ggcttccagc gccgccacgg cattaacgcg caattcaaaa gtccaggcac ctttgccgaa
    14881 ggtatcaaaa tcggctgcct gattaccttt atgcacctgc tgaaccactt tattcaggcg
    14941 cgcaacttcc tggcccagaa ccatcggaat agcatcgacc agtgtttgat aaatcacctc
    15001 tccgcccggc gcttcaccaa tgccggtatg cccggcgtta tcggtgagta ccacaatatt
    15061 gcgggtgaaa tatgcgttat gtgccccacc aatattaagc aacatgctat cgtgcccggc
    15121 gaccggaatg actttcatat cagtaataac aggactggat tgtgtcgtca tcttattgcc
    15181 ccgcgacagg tttcagctcg atacgcttga tatcgcccac cagcaccagg tagctcagta
    15241 ccgcgattaa ggcatgaaca ccaacataaa tcagcgcccc attaaaggag ccagtcgtgc
    15301 caacgatata accaattgcg attggcgtga cgatgccaga aatgttaccg aacatgttga
    15361 acaggccacc ggaaagaccg ctgatctctt ttggcgcggt atctgccatt actgcccaac
    15421 ccagcgcccc gatgccctta ccgaagaagg ccagcgccat aaagccgatg atcatccact
    15481 caacgttgac gtagttgcag aacaccatca ccatcgacag caacatgccc attacgatcg
    15541 gtgttttacg cgcaatgttc agcgatcccg tgcggcgcat cagccagtcg gaaataatcc
    15601 cacccagcac accgccgata aaaccgcaaa ccgccggaac ggaagccaca aagcccgctt
    15661 tcagaatcga catcccacgc gcctgcacca gataaaccgg aaaccaggta atgaaaaagt
    15721 aagtcagggc gttgatacag tactgaccga tataaacgcc gatcatcatc cgtgacccga
    15781 gcagttgttt gatctgcccc cacttcacgc tgaacggcac tttaactttg gtgttttgct
    15841 gatccatatt gatcagcgcg ccacccgcgg cgatatactc cagctctttc tgatttaccc
    15901 ccggatgttg atttggctcg tggatgactt tcaaccagat aaagctgatg acaatcccca
    15961 gaccgcccat aaagaagaag acgtgtgacc agcccacttc atgcgtcagc cagcccataa
    16021 tcggcgcgaa gatcaccgtt gcgaagtatt gagcggagtt aaaaatcgac accgccgttc
    16081 ccctttcctg cgccggaaac caggccgcaa caatgcgact gttgccgggg aaagatggcg
    16141 cttcagcaag cccgaccagg aagcgcagag taaacagggc aacgataatg ccgaatccac
    16201 taaagatatc gacgaagcct tgcagcaagg taaacatcga ccagataaag atcgaccaga
    16261 agtagacgcg ttttgaacca aaacggtcca gcaaccagcc accagggatc tgcccgataa
    16321 cataagccca tgagaaggca gagaacacat agcccattcc cacgggatca aggccgatat
    16381 ctttggccat ttccgaaccg gcaatagaaa gcgtagcgcg gtcgccgtag ttgaaggatg
    16441 tgacgataaa caacatcacc actatccagt aacgagcatt cgtgcgtttt tccacactgc
    16501 tcgcagcctg acttaaagaa ctcattgttg cactcctgaa aattcgcgtt agccacgctc
    16561 actctggact gcgacatagc caggaaatca gaggtgacgt agggtgtttt ttgccgtttt
    16621 tataggtcgt tcgccgaata cggcgcgtgt ttatatctgg caatagcagt ataaaaagcg
    16681 cgccatagcg gctcaccgtg caacaacaca acattaatgc gttcaatgag gcccgatttt
    16741 ggcattagcc ctggacggtg gaatccactt cacggaaatg aaaacaagaa caagaaagga
    16801 agggttaaaa cgaagaaata aagaagagta tgaaatggat cgcttgactc caggcaaacg
    16861 ccagtaaaaa tccgcgctat gaagcagttt ttactggcat ttgcctgaaa agattcgatt
    16921 cagcaccgct aaaacgacat ttaccgctcg ctgaacatat caggacaaca gcgtgcccca
    16981 ctgttcgacc cacggatttg attccgtttc cggttccggg ttctcactgg cgtcaatcaa
    17041 cagtatttcg ccaacccgct gagcgctctg ttcctgcaac aaggcatcga actgtttgcc
    17101 gccattgcag aaatttacat aactgctgtc gccgagcgca atcacgccat aacgcagatt
    17161 cggctggaag cccagactat ctttgattcc ctgaaagagc ggcacaatgc tatcaggaag
    17221 gtcgccctgc ccggtcgtgg acgtaaccac cagaacatac ttatcctgat aaggcagcca
    17281 gtcgcttaat tcaggatctt caaataccgt tgctttgtgg ccctgtgcgg tcagaattgc
    17341 ttctgcttct tccgccacta acagcgaatt tccgtacatg gtgccgacaa aaataccaat
    17401 ttccgccata ccgttttctc cctggattag gaacttatct ctccatcctg acccgatggc
    17461 gcactgaact caaccctttc attttcagga agcagaccgc gccagccaaa ttgtgataac
    17521 gcctgcatcc aggtgtcgtc caggcccgcg tgaatagtca gcggctcgcc agtaaaagga
    17581 tgcgtcaatg acaactgact ggcatgtagc attaaccgct ggaggccaaa atgctcagca
    17641 ccgctgcgat tctggcgtaa atcgccatgt ttgctatcgc caataatcgg atgacgcaaa
    17701 tgggcaagat gtcggcgaag ctgatgtttg cgtccggttt tcggctccag ttccaccagg
    17761 ccgtagcgcg tggtcgggta acgtccggtc gctaccggca tttctacggt cgccagaccg
    17821 cgataatgcg tcactgctgg ctgcgagcct ttatcttcgc gggcaaattt atcagcgatt
    17881 ttgtccagtt cttccaccag tggataatcc agaactgctt cttccatcaa ccagccgcgc
    17941 acaatcgcat ggtaacgttt ctggatttgg tgctgttcaa actgttgtgc cagcagccgt
    18001 ccggcctcgc tggataatcc catcaacaac acaccagaag tgggtcggtc cagacgatga
    18061 gcggtaaaaa cgtgctggcc aatctgatca cgcacggttt gcatgaccac tactttctcg
    18121 tcgcgatcca gccagctgcg gtgaaccagc cagccggagg gtttatttac cgcaaccagc
    18181 cattcatcct gatagagtat ttccagcatt agctcgcatc atccgcaaaa agagcatcca
    18241 gtttttccaa ctcagccaga ataagcgcgc gttgcggatg gtccgtcgcc agcgccattt
    18301 cataataggg tgcaacagca aaagcgcccg gtaacggctg gttgttattt aacaaatcgt
    18361 gcattcgcgg gatcagcacc cactgcaacc actccagtgg ttccatggtg tccataaaga
    18421 acggttgggt actattaaat tgatgcggct ggggttcatc gtttcgccag tgctgatgtt
    18481 cacgcagtaa cgcttcaagc gcctgcaact ggagacgaac gcggtcatga gtggtcataa
    18541 agcaacctca ataagaaaaa ctgccgcgaa gaatagcagg aataaaaaaa gggagcactg
    18601 tattcacagc gctcccggtt cgtttcgcag cattccagct acttttgttg ctccctgctc
    18661 atccttgaca acttttcctc tggccttgcg gccaatcgtt catcctgaac tattgcttcc
    18721 tgctcacacc accccgatgt gatacttcat cctgaagtgt ccctggcctt cctgacccac
    18781 cgaatcatcc tgaccggttc tcattctcca tcctggaggt gtcctttaac gcgtcctgcg
    18841 tcatcctctt cgcttcatcc agaaaccttt ccctgaaaca ccatcctggt gtgtcctgca
    18901 gaagtgtcat catcctgatg ttcacttcgt tgtctgactc cctgtcgacg aagatagaat
    18961 cgtctttttc gcgaagtctt acaaggcgct tacagacaat gccttaagga aaatttcata
    19021 tgaaagttaa gaattaacaa tcaacatttt gattttaaat gaaaataacg attcttatct
    19081 ttttaagtat tcgcttattt cctggcgatc tcctacagag tctgtaagag atctctcaca
    19141 accagatggg gtatttggat tacagaagag gcttaagttg attaaggaat tccgcaagat
    19201 ttgaggctaa atgcgtccgt ttacgtgttc ccagcgtctc tttgcacact tcaccggaga
    19261 gattacacac cgagatgacc tctaactctt cttccagcgt cgcgataaat agcgtgggcg
    19321 gcagttttag ccttttctga gtcaccagat gaccaatcag attttcctgc acccgacgga
    19381 agtcgtcttc actccaggtt tgcagcaatg tcagtttgat atcgccaaac tgcgcgtgca
    19441 tatccccggc aaactgagtc gtataaaagg tatgaattgt gggttgtatc acaatatcaa
    19501 aagcgcgttc aaccgcgttt acattttgtt ccccggtaaa cggctggggt tgccagtata
    19561 cggcatcttc ggtagttgaa ataatgcacg gtgaaggaac accgtacagt tcctcactta
    19621 acggccagct tttatgctct tcatgccacg catcgcagta gcgtgcagta aaatctttca
    19681 gggcttgtgc ggtcaaatcg tccactaatt tctctcttca cttaaaccag atacacttgt
    19741 cgtttagttt atctggttta tgacggtgaa acatgtcttc ttatgcaaac catcaggcac
    19801 ttgcgggcct gactcttgga aaatcaaccg attaccggga tacctatgac gccagcctac
    19861 tgcaaggcgt tccacgcagc ctgaatcgcg acccgctggg tctgaaagcg gataacctgc
    19921 cttttcacgg tacggatatc tggacgctgt atgaactttc ctggctgaat gcgaaaggtt
    19981 tgccgcaggt tgccgtcggt cacgttgaac ttgattacac cagcgcaaat ctgattgagt
    20041 cgaagagttt taagctctat ctcaacagtt ttaaccagac gcgttttaat aactgggatg
    20101 aggtgcgcca gacgctggag cgcgacttaa gcacttgcgc ccagggtaag gttagcgtgg
    20161 cgttatatcg tcttgatgaa ctggaaggcc agccgatagg tcattttaat ggcacttgca
    20221 ttgatgacca ggatatcact atcgataact atgaattcac tactgactat ctggagaatg
    20281 ccacctgtgg tgaaaaagta gtggaagaga cgcttgtcag ccacctgctg aaatcaaact
    20341 gcctgatcac ccatcaacca gattggggtt cgatccaaat tcagtatcgt ggacgccaaa
    20401 ttgacagaga aaaactgctg cgttacctgg tctcattccg tcatcacaac gagttccacg
    20461 aacagtgcgt ggaacgcatc tttaatgacc tgttacgctt ctgccagcca gaaaaattga
    20521 gcgtttacgc acgttatacc cgtcgtggcg gtctggacat taacccgtgg cgcagtaata
    20581 gcgattttgt cccatcgacc acaagactgg ttcggcaata aattttttct caattttgcg
    20641 tgctggattc acgcagaagg ttgtgaaagg tcatcaggca gggctattgt aatcaaaggg
    20701 aatgacgata ttcgtcccat aaggagtttt tcttgattac acatattagc ccgcttggct
    20761 ccatggatat gttgtcgcag ctggaagtgg atatgcttaa acgcaccgcc agcagcgacc
    20821 tctatcaact gtttcgcaac tgttcacttg ccgtactgaa ctccggtagt ttgaccgata
    20881 acagcaaaga attgctgtct cgttttgaaa atttcgatat taacgtcctg cgccgtgaac
    20941 gcggcgtaaa gctggaactg attaatcccc cggaagaggc ttttgtcgat gggcgaatta
    21001 ttcgcgcttt gcaggccaac ttgttcgcgg ttctgcgaga cattctcttc gtttacgggc
    21061 aaatccataa taccgttcgt tttcccaacc tgaatctcga caactccgtc cacatcacta
    21121 acctggtctt ttccatcttg cgtaacgctc gcgcgctgca tgtgggtgaa gcgccaaata
    21181 tggtggtctg ctggggcggt cactcaatta acgaaaatga gtatttgtat gcccgtcgcg
    21241 tcggaaacca gctgggcctg cgtgagctga atatctgcac cggctgtggt ccgggagcga
    21301 tggaagcgcc gatgaaaggt gctgcggtcg gacacgcgca gcagcgttac aaagacagtc
    21361 gttttattgg tatgacagag ccgtcgatta tcgccgctga accgcctaac ccgctggtca
    21421 acgaattgat catcatgccg gatatcgaaa aacgtctgga agcgtttgtc cgtatcgctc
    21481 acggcatcat tatcttccct ggcggtgtgg gtacggcaga agagttgctg tatttgctgg
    21541 gaattttaat gaacccggcc aacaaagatc aggttttacc attgatcctc accggcccga
    21601 aagagagcgc cgactacttc cgcgtactgg acgagtttgt cgtacatacg ctgggtgaaa
    21661 acgcgcgccg ccattaccgc atcatcattg atgacgccgc tgaagtcgct cgtcagatga
    21721 aaaaatcgat gccgctggtg aaagaaaatc gccgtgatac aggcgatgcc tacagcttta
    21781 actggtcaat gcgcattgcg ccagatttgc aaatgccgtt tgagccgtct cacgagaata
    21841 tggctaatct gaagctttac ccggatcaac ctgttgaagt gctggctgcc gacctgcgcc
    21901 gtgcgttctc cggtattgtg gcgggtaacg taaaagaagt cggtattcgc gccattgaag
    21961 agtttggtcc ttacaaaatc aacggcgata aagagattat gcgtcgtatg gacgacctgc
    22021 tacagggttt tgttgcccag catcgtatga agttgccagg ctcagcctac atcccttgct
    22081 acgaaatctg cacgtaatct cccctccccc ggtgacgttt gttgccgggt tttctcgttt
    22141 ttggtcactt actcatcaac tcatttcatt tgttatatga atgtttctta ccaccctcac
    22201 gcgacaaata tcatcacagt taatatgtca tacaatttat gttgcaatgc aaacgtttcc
    22261 ctattttcat aaaccgtatt ttatcgctat gaaaaagaaa ttatcgccat gattaactaa
    22321 aagtattgat tttttcagtt caacctacat atattgcgcg cccaggaaga agtcagatgt
    22381 cgtttaatgg gcaaatattg cccttaaatt ctcttttact tttgatttac agagtaaagc
    22441 gttgggataa tctatcttcc aagtagatta ttgtatttgg gatcaagatc actgatagat
    22501 acataacttg tgtgtatctt tccgccctca aattattacg gcggtaaatg attaagccat
    22561 cgccgataga cagatttcat ttttacggtc aggcaccttc ccgggctgaa ctggctaaaa
    22621 gctgaattat ttgcattcct ccaggagaaa tagatggaaa cgactcaaac cagcacgatt
    22681 gcgtcgaaag actctcgtag tgcctggcgc aagacagaca ccatgtggat gctgggcctt
    22741 tacggcacgg caatcggcgc gggcgtgctg ttcctgccaa tcaacgccgg tgttggcggt
    22801 atgatcccgc tgatcatcat ggctatcctt gcgttcccga tgacgttttt tgctcaccgc
    22861 ggcctgactc gcttcgtact gtctggtaaa aacccgggcg aagacatcac cgaggttgta
    22921 gaagaacact ttggtattgg cgcaggtaaa ctgattaccc tgctctactt cttcgctatc
    22981 tacccgatcc tgctggttta tagcgtggca atcaccaata cagttgaaag cttcatgtct
    23041 caccagctgg gtatgacgcc accgccgcgt gcgattctgt cgctgatcct gatcgtgggt
    23101 atgatgacca tcgttcgctt tggtgagcag atgatcgtta aagcgatgag tattctggta
    23161 ttcccgtttg ttggcgtact gatgctgctg gctctgtacc tgatcccgca gtggaacggc
    23221 gcagcactgg aaacgctgtc tctggacact gcatctgcaa ccggaaacgg tctgtggatg
    23281 accctgtggc tggcaattcc ggtaatggtg ttctcgttca accactctcc gatcatctct
    23341 tctttcgccg ttgcgaagcg tgaagagtac ggcgatatgg cagaacagaa atgctcgaag
    23401 atcctggcat tcgcacacat catgatggtg ctgaccgtaa tgttcttcgt cttcagttgc
    23461 gtactgagcc tgactccggc agacctggct gcggctaaag agcagaacat ctcgattctg
    23521 tcttacctgg ctaaccactt taacgcaccg gttatcgcgt ggatggctcc gattatcgcg
    23581 attatcgcta tcaccaaatc cttcctcggc cactacttgg gcgcacgtga aggcttcaac
    23641 ggtatggtga ttaaatctct gcgtggtaaa ggtaagtcta tcgaaatcaa caagctgaac
    23701 cgtatcactg cgctgttcat gctggtaacg acctggattg ttgccaccct gaacccgagc
    23761 atcctgggta tgattgaaac cctgggcggc ccaatcatcg cgatgatcct gttcctgatg
    23821 ccgatgtacg caattcagaa agtaccggca atgcgtaagt acagcggtca catcagcaac
    23881 gtattcgttg tcgtgatggg tctgattgca atctccgcaa tcttctactc tctgttcagc
    23941 taagtccttt cgcgccgctt tcgggcggcg cttcctccgt tttaacgcga tgtatttcct
    24001 atgattagcg tattcgatat tttcaaaatc ggcattggcc cttccagttc tcataccgtt
    24061 ggaccaatga aagcgggtaa acaatttacc gacgatctga ttgcccgtaa tctgcttaaa
    24121 gacgtgaccc gcgtggtggt tgacgtgtac ggctcgctct ctctgaccgg taaaggccac
    24181 cacactgata tcgccattat tatgggcctg gcgggtaacc tgccggatac cgtggatatc
    24241 gattccatcc ctggttttat tcaggatgtg aatactcatg gtcgcctgat gctggcaaac
    24301 ggtcagcatg aagtggagtt cccggttgat cagtgcatga acttccatgc agacaacctt
    24361 tctctgcatg aaaacggtat gcgcattacc gcgctggcgg gcgataaagt cgtttacagc
    24421 cagacttact actctattgg cggtggcttt atcgttgatg aagagcattt tggccagcag
    24481 aatagcgcac cggttgaagt tccttatccg tacagttcag cagccgatct gcaaaaacat
    24541 tgtcaggaaa ccgggctgtc actctctggc ctgatgatga aaaacgagct ggcgctgcac
    24601 agcaaagaag agctggaaca gcacctggcg aacgtctggg aagtcatgcg cggcggtatt
    24661 gagcgcggta tttctaccga aggcgtgttg cctggcaaac tgcgcgtacc acgccgtgct
    24721 gcggcactac gccggatgct ggtcagccag gataaaacca ccactgaccc gatggcggtt
    24781 gttgactgga tcaacatgtt tgcactggca gtgaacgaag agaacgccgc tggcggtcgc
    24841 gtggtgactg cgccgactaa cggtgcgtgc gggattatcc cggctgtgct ggcgtactac
    24901 gataagttta tccgcgaagt gaacgctaac tcactggctc gttacctgct ggtagccagt
    24961 gccattggtt ctctttataa gatgaacgcg tcaatttctg gtgctgaagt cggctgccag
    25021 ggtgaagttg gtgtggcgtg ctcaatggcg gcggctggtc tggcagaact gttaggtgca
    25081 agcccggcgc aggtgtgcat cgcggcggaa atcgccatgg agcacaacct cggtctgacg
    25141 tgtgacccgg tcgccggaca ggtccaggtg ccatgcatcg agcgtaacgc cattgcggca
    25201 gtaaaagcgg tgaacgccgc acgtatggcg ctacgccgta ccagcgagcc gcgcgtctgc
    25261 ctcgataaag ttatcgaaac catgtacgaa acaggtaaag atatgaacgc caagtaccgc
    25321 gaaacctctc gcggcggcct ggcaatgaag atcgttgcct gcgattaatc gctctccaga
    25381 ggcctcgttt tgcgaggcct cttcccgatt tctcatccag ccgtagcctg ttccggcatc
    25441 gaatgttacc cttatcgcct gatctttaag ggggttatcg tggctgttca tttgcttatt
    25501 gtcgatgcac tgaatcttat tcgtcgcatt catgccgttc agggatcgcc ctgtgtcgaa
    25561 acctgccagc atgcgctcga tcagctcatt atgcacagcc agccaaccca cgcggtcgcc
    25621 gtttttgatg atgaaaaccg cagtagcggc tggcgtcatc agcgtttacc agattacaaa
    25681 gcgggtcgac cgccaatgcc ggaagagttg cacgacgaga tgcctgcatt acgcgccgcc
    25741 tttgagcaac gcggcgtccc gtgctggtca accagcggca acgaagccga tgacttagcc
    25801 gccacgctgg cggtcaaagt gacacaggcc gggcatcagg caacgattgt ttcgacagat
    25861 aaaggctact gtcagttact ttcaccgaca ttacgtattc gtgattactt ccagaaacgt
    25921 tggctggatg cgccatttat cgataaagaa tttggcgttc aaccgcagca gttgcccgat
    25981 tactggggac ttgcggggat cagcagttca aaggtaccgg gtgttgcggg aatcggacca
    26041 aaaagcgcca cgcagctgct ggtcgagttt cagagtctgg aagggatata tgagaatctg
    26101 gatgcggttg ccgaaaagtg gcgcaaaaaa ttagaaaccc ataaagagat ggcgtttctg
    26161 tgccgcgata ttgcccgctt acaaaccgat ttgcatatcg acggcaattt acagcaattg
    26221 cggttggtac ggtaacggcg agccggatac gccgcaaacg tcgtatccgg cattatcaca
    26281 tcagcgcatt taccaggcgg tatggtaaag ctctacaata tcctcaagcg ttgcttcacg
    26341 cgggttgcca ccggtacaaa catcatccag tgccgcctgc gccagtgccg gaatgtcttc
    26401 cttgcgtaca ccaacatcac gcaaatgtgg cggaataccg acatcacggt tgagagcaaa
    26461 caccgcttca acagcggcat tacgcgcctc ttccaggctc ataccttcca ctttcacgcc
    26521 cataacgcgc gcgatatcgc ggtacttctc accggtaaag tcagcgttat agcgcatgac
    26581 atgcggtaac aggatggcgt tcgcaacacc gtgtggagtg ttgtaaaacg cgcccagtgg
    26641 atgcgccata ccatgcacca accctaaccc aacattcgag aagcccatac ccgcaacata
    26701 ctgcccgagc gccatttctt ctccggcatc cttatcacca gcaaccgatc ctcgcagcgc
    26761 cccagcaatg atttcaatcg ctttaatgtg cagtgcatcg gttagcgccc acgcgccacg
    26821 ggtaatatac ccctcaatag catgagtgag cgcatcgaca cccgtcgcag ctttcagcgc
    26881 tggaggcata ccatccatca tgtcagcgtc aataaacgcc acctgcggga tatcatgcgg
    26941 atcaacgcaa acaaacttgc gccgtttctc ttcgtcagtg atcacgtagt taatggtcac
    27001 ttctgccgca gtacctgctg tggtaggaat tgccagaatc ggtacactgg gtttattggt
    27061 cggggaaagc ccttccaggc tacgcacatc ggcaaactcc gggttgttgc tgataatgcc
    27121 aatcgcttta caagtatcct gtggagaacc accaccaata gcgatcaggt aatccgcgcc
    27181 gctattctgg aatacaccga gcccttcttt gacgacagta attgttgggt tgggcactac
    27241 gccgtcgtaa atcgcccatg ccagccctgc agcatccatc ttatcggtca ctttcgccac
    27301 cacgccgcat tgcaccagcg ttttatcggt gacgatcagc gccttctgat aaccacggcg
    27361 tttcacctca tcggttaaag ccccaacagc accccgacca aaccatgccg tttcgttcag
    27421 aatcattctg ttagccatca tccttctcct tgttgcttta cgaaattact cttcaatccg
    27481 taacccatag gttttgaatt tctccagcac tacggcaatc tcttcatcgc tcagcactgg
    27541 caccgggtcc gtaatcgcca gggtcgtcag gtaaagttgc gccagcactt caacttcatg
    27601 cgccagccat aacgcttttt ccagattcac ctcacaagcg ataagcccat gatgttgtaa
    27661 caaagttgcc ttacgatttt tgagagcctg cgcaacatgt tcagaaagtt cacgtgttcc
    27721 aaaggtcgca taaggcgcgc aaggaataga attaccgcca gccgccgcaa tcatgtagtg
    27781 aatagcgggg atcgatcggt taagaatgga aactgccgtg caatgaacgg catgattgtg
    27841 aacaaccgcg ttggcatccg gtctgctttg ataggccgcc atatggaaac gccattcgct
    27901 tgaggggagc tttccttcct catgtttacc gttgccatca ataaagacaa tatgcgactc
    27961 cgtcagtttt tcatatggaa tgcctgtagg cgtaatcagc atcccatcct gataacgtac
    28021 actgacgttc cccgctgtcc cctggttcag tcccaggcgg gtcatttcca ggcaagtgtc
    28081 aataatctga cgagcaagtt tatttcgttc cattagctac ctctctctga ttcaaaacag
    28141 ggcaataatg ttgttccttt cacactattg aattagccgt ttaattaccc accatcttct
    28201 tcctgattaa ccagaaagaa attcacaagc ttatattttg tgacctggtt caactaatca
    28261 cagtaaataa ctgcaagttc tctttttata accccattaa aaatgaccgc tcttaaaaat
    28321 atttatcaaa acggtcattt ttctattcac tcaagccagg aatgcccgtt ttcggcataa
    28381 acaattaata cggtcatctg gtttgtgttt tttatgattt attttctgaa acgggcatga
    28441 aatttcgatt attaaagtga tggtagtcac ataaagtcac cttccagcta ataagtgtga
    28501 ccgccgtcat attacagagc gttttttatt tgaaaatgaa tccatgagtt catttcagac
    28561 aggcaaatat tcactgatat gaagcccgaa ctcgctggtt ttgcactttt gaaaacataa
    28621 ccgattacgt gcttaagctt ctgaacctaa gaggatgcta tgggaaacac atcaatacaa
    28681 acgcagagtt accgtgcggt agataaagat gcagggcaaa gcagaagtta cattattcca
    28741 ttcgcgctgc tgtgctcact gttttttctt tgggcggtag ccaataacct taacgacatt
    28801 ttattacctc aattccagca ggcttttacg ctgacaaatt tccaggctgg cctgatccaa
    28861 tcggcctttt actttggtta tttcattatc ccaatccctg ctgggatatt gatgaaaaaa
    28921 ctcagttata aagcagggat tattaccgga ttatttttat atgccttggg cgctgcatta
    28981 ttctggcccg ctgcagaaat aatgaactac accttatttt tagttggcct atttattatt
    29041 gcagccggat taggttgtct ggaaactgcc gcaaaccctt ttgttacggt attagggccg
    29101 gaaagcagtg gtcacttccg cttaaatctt gcgcaaacat ttaactcgtt tggcgcaatt
    29161 atcgcggttg tctttgggca aagtcttatt ttgtctaacg tgccacatca atcgcaagac
    29221 gttctcgata aaatgtctcc agagcaattg agtgcgtata aacacagcct ggtattatcg
    29281 gtacagacac cttatatgat catcgtggct atcgtgttac tggtcgccct gctgatcatg
    29341 ctgacgaaat tcccggcatt gcagagtgat aatcacagtg acgccaaaca aggatcgttc
    29401 tccgcatcgc tttctcgtct ggcgcgtatt cgtcactggc gctgggcggt attagcgcaa
    29461 ttctgctacg ttggcgcaca aacggcctgc tggagctatt tgattcgcta cgctgtagaa
    29521 gaaattccag gtatgactgc aggctttgcc gctaactatt taaccggaac catggtgtgc
    29581 ttctttattg gtcgtttcac cggtacctgg ctcatcagtc gcttcgcacc acacaaagtc
    29641 ctggccgcct acgcattaat cgctatggca ctgtgcctga tctcagcctt cgctggcggt
    29701 catgtgggct taatagccct gactttatgc agcgccttta tgtcgattca gtacccaaca
    29761 atcttctcgc tgggcattaa gaatctcggc caggacacca aatacggttc gtccttcatc
    29821 gttatgacca tcattggcgg cggtattgtc actccggtca tgggttttgt cagtgacgcg
    29881 gcgggcaaca tccccactgc tgaactgatc cccgcactct gcttcgcggt catctttatc
    29941 tttgcccgtt tccgctctca aacggcaact aactgaacat attttccgaa taaagtgagg
    30001 aatctgtaat gaaaaaaatc agcttaccga aaattggtat ccgcccggtt attgacggtc
    30061 gtcgcatggg tgttcgtgag tcgcttgaag aacaaacaat gaatatggcg aaagctacgg
    30121 ccgcactgct gaccgagaaa ctgcgccatg cctgcggagc tgccgtcgag tgtgtcattt
    30181 ccgatacctg tatcgcgggt atggctgaag ccgctgcttg cgaagaaaaa ttcagcagtc
    30241 agaatgtagg cctcaccatt acggtaacgc cttgctggtg ctatggcagt gaaaccatcg
    30301 acatggatcc aacccgcccg aaagccattt ggggctttaa cggcactgaa cgtcccggcg
    30361 ctgtttacct ggcagcggct ctggcagctc acagccagaa aggcatcccg gcattctcca
    30421 tttacggtca tgacgttcag gatgccgatg acacatcgat tcctgccgat gttgaagaaa
    30481 aactgctgcg ctttgcccgc gccggtttgg ccgtcgccag catgaaaggt aaaagctatc
    30541 tgtcgctggg cggcgtttcg atgggtatcg ccggttctat tgttgatcac aacttctttg
    30601 aatcctggct gggaatgaaa gtccaggcgg tggatatgac cgaactgcgt cgccgtatcg
    30661 atcagaagat ttacgacgaa gccgaactgg aaatggcact gacctgggct gataaaaact
    30721 tccgctatgg cgaagatgaa aataacaaac agtatcaacg caatgccgag caaagccgcg
    30781 cagttctgcg cgaaagttta ctgatggcga tgtgtatccg cgacatgatg caaggcaaca
    30841 gcaaactggc cgatattggt cgcgtggaag aatcacttgg ctacaacgcc atcgctgcgg
    30901 gcttccaggg gcaacgtcac tggaccgatc aatatcccaa tggtgacacc gccgaagcga
    30961 tccttaacag ttcatttgac tggaatggcg tgcgcgaacc ctttgtcgtg gcgaccgaaa
    31021 acgacagtct taacggcgtg gcaatgctaa tgggtcacca gctcaccggc accgctcagg
    31081 tatttgccga tgtgcgtacc tactggtcac cagaagcaat tgagcgtgta acggggcata
    31141 aactcgatgg actggcagaa cacggcatca tccatttgat caactccggt tctgctgcgc
    31201 tggacggttc ctgtaaacaa cgcgacagcg aaggtaaccc gacgatgaag ccgcactggg
    31261 aaatctctca gcaagaggct gacgcttgcc tcgccgctac cgaatggtgc ccggcgatcc
    31321 acgaatactt ccgtggcggc ggttactctt cccgcttcct taccgaaggc ggcgtcccgt
    31381 tcaccatgac ccgtgtcaac atcatcaaag gcctgggacc ggtactgcaa atcgcggaag
    31441 gctggagcgt ggaattgccg aaggatgtgc atgacatcct caacaaacgc accaactcaa
    31501 cctggccaac cacctggttt gcaccgcgcc tcaccggtaa agggccgttt acggatgtgt
    31561 actcggtaat ggcgaactgg ggcgctaacc atggggttct gaccatcggc cacgttggcg
    31621 cagactttat cactctcgcc tccatgctgc gtatcccggt atgtatgcac aacgttgaag
    31681 agaccaaagt gtatcgtcct tctgcctggg ctgcgcacgg catggatatt gaaggccagg
    31741 attaccgcgc ttgccagaac tacggcccgt tgtacaagcg ttaatacatt actccctgat
    31801 gtgatgcccg gtcgctccgg ctaccgggcc tgaacaagca agagtggtta gccggataag
    31861 caatgttatc cggctatatt gcaggagcga ttatgaaaca agaagttatc ctggtactcg
    31921 actgtggcgc gaccaatgtc agggccatcg cggttaatcg gcagggaaaa attgttgccc
    31981 gcgcctcaac gcctaatgcc agcgatatcg cgatggaaaa caacacctgg caccagtggt
    32041 ctttagacgc cattttgcaa cgctttgctg attgctgtcg gcaaatcaat agtgaactga
    32101 ctgaatgcca catccgcggt atcgccgtca ccacctttgg tgtggatggc gctctggtag
    32161 ataagcaagg caatctgctc tatccgatca ttagctggaa atgtccgcga acagcagcgg
    32221 ttatggacaa tattgaacag ttgatctccg cacagcggtt gcaggctatt tctggcgtcg
    32281 gagcctttag tttcaatacg ttatataagt tggtgtggtt gaaagaaaat catccacaac
    32341 tgctggaacg cgcgcacgcc tggctcttta tttcgtcgct gattaaccac cgtttaaccg
    32401 gcgaattcac tactgatatc acgatggccg gaaccagcca gatgctggat atccagcaac
    32461 gcgatttcag tccgcaaatt ttacaagcca ccggtattcc acgccgactc ttccctcgtc
    32521 tggtggaagc gggtgaacag attggtacgc tacagaacag cgccgcagca atgctcggct
    32581 tacccgttgg cataccggtg atttccgcag gtcacgatac ccagttcgcc ctttttggcg
    32641 ctggtgccga ccaaaatgaa cccgtgctct cttccggtac atgggaaatt ttaatggtcc
    32701 gtagcgccca ggttgatact tcgctgttaa gtcagtacgc cggttccacc tgcgaactgg
    32761 atagccaggc agggttgtat aacccaggta tgcaatggct ggcatccggc gtgctggaat
    32821 gggtgagaaa actgttctgg acggctgaaa caccctggca aatgttgatt gaagaagctc
    32881 gtctgatcgc gcctggcgcg gatggcgtaa aaatgcagtg tgatttattg tcgtgtcaga
    32941 acgctggctg gcaaggagtg acgcttaata ccacgcgggg gcatttctat cgcgcggcgc
    33001 tggaagggtt aactgcgcaa ttacagcgca atctacagat gctggaaaaa atcgggcact
    33061 ttaaggcctc tgaattattg ttagtcggtg gaggaagtcg caacacattg tggaatcaga
    33121 ttaaagccaa tatgcttgat attccggtaa aagttctcga cgatgccgaa acgaccgtcg
    33181 caggagctgc gctgttcggt tggtatggcg taggggaatt caacagcccg gaagaagccc
    33241 gtgcacagat tcattatcag taccgttatt tctacccgca aactgaacct gaatttatag
    33301 aggaagtgtg aaatgctgaa aacaatttcg ccgttaattt ctcccgaact attgaaagtg
    33361 ctggcagaga tgggacatgg agatgaaatt attttttccg atgctcactt tcccgcccat
    33421 tcgatgggac cgcaggtgat ccgcgctgat ggcctgttgg tgagcgactt gctccaggcg
    33481 attatcccgt tatttgagct ggacagttat gcaccgccgc tggtgatgat ggcagcggta
    33541 gaaggtgaca ctctcgatcc tgaagtagaa cgacgttacc gtaatgcgct ttcactacaa
    33601 gccccgtgtc ctgacatcat ccgcatcaat cgttttgcgt tttatgaacg ggcgcaaaaa
    33661 gcctttgcga tcgttatcac aggcgaacga gcgaagtacg ggaatattct tttaaaaaaa
    33721 ggggtaacac cgtaatctca taccggtacg cccgcatgac gcgggcggtt atcgaatgat
    33781 ggggtgaaaa atatgaaagc ggcacgccag caagcgatag tcgacctgct gctgaaccat
    33841 accagcctga ccacggaagc tctctctgaa cagctaaagg tcagtaaaga aaccattcgt
    33901 cgcgatctca atgaattaca gacgcagggt aaaattctgc gcaatcatgg acgcgctaaa
    33961 tatatccacc gtcaaaatca agacagtggc gatccctttc acatccggct gaaaagccat
    34021 tatgcgcata aagcagatat cgcgcgcgag gcgctcgcgt ggattgaaga agggatggtg
    34081 atagccttag acgccagttc aacttgctgg tatctggcac gccagttgcc tgacatcaac
    34141 attcaggtct tcaccaatag ccatccgatt tgccatgaac tcggtaaacg cgaacgcatt
    34201 caactgatca gttccggcgg cacacttgag cgcaaatatg gctgttacgt caatccctcg
    34261 ctgatttccc aacttaaatc gctggaaatc gatctgttta ttttttcttg tgaagggatc
    34321 gatagcagcg gcgcattgtg ggactccaat gccatcaacg ctgattacaa atcgatgcta
    34381 ttaaaacgtg ccgcgcaatc gttgttattg attgataaaa gtaaatttaa tcgttcaggg
    34441 gaagcccgca tcgggcatct ggatgaggta acgcacatta tttctgatga gcgccaggtt
    34501 gcaacttctt tggtaacagc ctgacggaaa gggtagcagg ccggagacga cgccccggcc
    34561 ttgcctgtta tcgctcgtcg cgacgaccac ccaccgcagc ccagatgcgg cggacgtgca
    34621 ccgtcacttc ttcgcgatcg tgatacaact gccgcgcctg aatctgagca tttatgccat
    34681 gttcatcaag ctgtgcctga atatacgcca gattgtgtga cacttcttcg tagcgttttt
    34741 tcatcggcag tttgaggttg aaaatggttt cacggcacca gccattaacc agccactgcg
    34801 ccatcaatgc cgcaactttc gccggttttt caaccatatc gcataccatc caggagatat
    34861 tgctgcgcgt cggacggaat ttgaaaccgt cttcccgcag ccacgtcacc tgtccggtat
    34921 ccatcagact ttgcgccatc gggccgttgt cgacggaata aacccacatg ttgcgcttca
    34981 ccagttggta ggtccagccg ccagggcaag cgcctaaatc caccgcccac atcccgttcg
    35041 ccaggcgttc atcccactca tccgcaggaa taaacacatg aaatgcctct tccagtttga
    35101 gcgtggaacg actcggcgca tctgccggaa atttcaggcg cggaatgccc atatagaacg
    35161 gcgaattatt gttgctgtat gagtaaccgg tatagcagca gcctggtgca ataaagaata
    35221 catgcacaac cggacgcttc ggcgtttcat agttcgccag caccccggca tcgcgcaagg
    35281 cagcgcgtag cggaacagta aatttacggc agaatttcag taactctttg ctttcgttgg
    35341 tatcggcaac ttcaacacgc agttcaccgc ccttctctac tacgccctgt aacatgccga
    35401 caatgggggt aatacgatct tctggcggca aatgctgcag gagttccccc accacaaacc
    35461 actggcgggc aaaaattaat gaactgaacg gcagctcacg gattaactta tcgccatcat
    35521 caggttgata acattcataa atgacataac ccgcattctc tttcacgcgg gcaaaaccga
    35581 aaatttcccg ctggccggct ttatcggtaa tttctgcggc gcactctttt tcaaagcccg
    35641 gacggcacag caatacaacc ttattcatga cttacgccct tgcgctttaa acggatagca
    35701 ccaactaaca ttaacgccca gcccgcgagg aagctcacgc cgccaaccgg agtgacaaac
    35761 gcccacaaac gcagatggga cagcgccagg caataaaggc tgccgctgaa caacaccgtg
    35821 cctaacgcga ggaaaacgct actccagtaa aaccagatac tgatgcgacg ctgcattgcc
    35881 accgccagac ctaagatcgc cagcgtatga aacgcctggt attcgaggcc ggtctggatc
    35941 cagcccatct caacggcccc catggtttta cttaacacat gcgcgccaaa agcgcccaga
    36001 gccacaaaaa tgaagccgct aatggcggcg aaaatcagca taaaacggct ggtcatggtc
    36061 gtaccctacg taaattattg ttcataacga aagcggaatt tttcttgttc agcagcagct
    36121 ttcgccagga tccattgacg aaaggcggct attttaccca gttctgcctg gctgtcatga
    36181 caaaccagat aaaaagcgtt tttactgacc agaacatcat taaacgggca aacaagacgt
    36241 ccggcctcga tttcagattg cgccatcacg ttatttgcca gcgccactcc ctgcccgtga
    36301 atagccgctt gcagcaccat ggcgctatgg ctaaaaattg gcccttgctg aacgttgata
    36361 tgatttaacc ccaattgtcg ggtatatgtc tgccagtcac ggcgtgacgc atcatgtaat
    36421 aacgtatgtt tagccagatc ttccggggtc ttcaagggtt tttcgccagt cagcagtagc
    36481 ggcgaacaca ccggcaataa atattcggcg taaagttttt ccacccgtag ccccggccag
    36541 ttgccccgac cataaaatat cgccacatca acatcatccg ccagcttatc ttcctgacga
    36601 tcaaccgcct ggattcgaac gtcaattccc ggataagctg aattaaagct ggaaagtcgc
    36661 ggaaccaacc aatgaatggc gaaactgggg agtaaactga ccgtcaacgc ccccttggcg
    36721 ctacgggcct ggagtttacg cgtcgcttcg gttaattgcg aaaatatctc tttgatatcg
    36781 aggaaatagc tttgcccttc ctcggtcagc aggagtgaac gattacggcg gcggaacagt
    36841 tttagcccca aaaaatcctc aagagacttg atttgatgac ttactgcggc ctgggtcaca
    36901 aaaagctctt ctgctgcgcg agtgaaactt aaatggcgtg ctgcggcatc aaaaactcgt
    36961 aaggcattta gcggtggtaa tcgtttagac atggctttta aactttgatg ttaaattaat
    37021 ttaacaatta gatcacacta tgtaacctat tagttttttt aatctgagcc attataaatt
    37081 gtccgttgag cttctaccag caaataccta tagtggcggc acttcctgag ccggaacgaa
    37141 aagttttatc ggaatgcgtg ttctggtgaa cttttggctt acggttgtga tgttgtgttg
    37201 ttgtgtttgc aattggtctg cgattcagac catggtagca aagctacctt ttttcacttc
    37261 ctgtacattt accctgtctg tccatagtga ttaatgtagc accgcctaat tgcggtgctt
    37321 ttttttacct tgcgatcgcg aattactgat ccagttcgac catctctttc acgtcagtac
    37381 ggttgatctg ctgtttgttg ccattagcgt ctttatacga aatcataccg gtatcgttat
    37441 cagtctgtgg tttgccgtca gagacgatgg tacgtccgtc attggtgtgc atcacatagt
    37501 tcgaaccgga acaggcgctc agggcaaaag tcagcataca ggcagaaata attgcggcag
    37561 tctttttcat catcttctcc ttaaagcgat taataaatct taatagtatt gattaacagg
    37621 ctaaaattaa cgcctaacac tattcagcat atgttactta cgcggcttcg ccaggatatc
    37681 cagataattc tgatggttag cactctcctt gtatcaaagt gaattttgcg tcacgatcgg
    37741 tgcatcaagc cgaggagtac catgaacgtt tttaatcccg cgcagtttcg cgcccagttt
    37801 cccgcactac tggatgcggg cgtctatctc gatagcgccg cgaccgcgct taaacctgaa
    37861 gccgtggttg aagccactcg acagttttac agcctgagcg ccggaaacgt ccatcgcagc
    37921 cagtttgccg aagcccaacg cctgaccgcg cgttatgaag ctgcacgaga gaaagtggcg
    37981 caattactga atgcaccgga tgataaaact atcgtctgga cgcgcggcac cactgaatcc
    38041 atcaacatgg tggcacaatg ctatgcgcgt ccgcgtctgc aaccgggcga tgagattatt
    38101 gtcagcgtgg cagaacacca cgccaacctc gtcccctggc tgatggtcgc ccaacaaact
    38161 ggagccaaag tggtgaaatt gccgcttaat gcgcagcgac tgccggatgt cgatttgttg
    38221 ccagaactga ttactccccg tagtcggatt ctggcgttgg gtcagatgtc gaacgttact
    38281 ggcggttgcc cggatctggc gcgagcgatt acctttgctc attcagccgg gatggtggtg
    38341 atggttgatg gtgctcaggg ggcagtgcat ttccccgcgg atgttcagca actggatatt
    38401 gatttctatg ctttttcagg tcacaaactg tatggcccga caggtatcgg cgtgctgtat
    38461 ggtaaatcag aactgctgga agcgatgtcg ccctggctgg gtggcggcaa aatgattcac
    38521 gaagtgagtt ttgacggttt caccactcaa tctgcgccgt ggaaactgga agcaggaacg
    38581 ccaaatgtcg ctggcgtcat aggattaagc gcggcgctgg aatggctggc agattacgat
    38641 atcaaccagg ccgaaagctg gagccgtagc ttagcaacgc ttgcagaaga agcgctggcg
    38701 aaacgtccag gctttcgttc attccgctgc caggattcca gcctgctggc ctttgatttt
    38761 gctggcgttc atcatagcga tatggtgacg ctgctggcgg agtacggtat tgccttgcgg
    38821 gccgggcaac attgcgctca gccgctactg gcagaattag gcgtaaccgg cacactgcgc
    38881 gcctcatttg cgccatataa tacaaagagt gatgtggatg cgctggtgaa tgccgttgac
    38941 cgcgcgctgg aattattggt ggattaatga caaacccgca attcgccgga catccgttcg
    39001 gcacaaccgt aaccgcagaa acgttacgca ataccttcgc tccgttgtcg caatgggaag
    39061 ataaatatcg ccagttgatc atgctgggga aacagcttcc ggctttgcca gacgagttaa
    39121 aagcgcaggc taaagagatt gccggatgcg aaaaccgcgt ctggctggga tataccgtga
    39181 ctgaaaacgg taaaatgcac ttctttggcg atagcgaagg gcgcattgtg cgcggcctgt
    39241 tggcggtgtt gttgactgcc gttgagggga aaaccgccgc cgaattgcag gcacagtcgc
    39301 cgctggcatt gtttgatgag ctgggattgc gtgcacagct tagcgcctca cgcagccagg
    39361 ggttgaatgc gttaagcgag gcgattatcg ctgcggcgaa gcaggtttaa gcacagaacc
    39421 aggtcggata agacgttcgc gtcgcatccg acctgattgt ttaaccctga cgcgccgctt
    39481 tcgccatcat cttcttcagc gcatgagaaa ccgcaacaaa cccaaaggtg gcggtcacca
    39541 tcgttgccgc gccaaatcca gatgcacaat ccatccgctt cggcccttct gccgtggctt
    39601 tcatcgcaca cacagtaccg tctgactgcg ggtacaccag cgcttcagta gaaaacacgc
    39661 aatcgacgcc gagcttacct ttactgtttt tcactacgcc aaaatcgctt ttcaggcgct
    39721 cgcgcaactt cgccgccagc ggatcctgaa tcgttttcgc cagatcggta acctgaatct
    39781 gcgtcgggtc aatctgccca cccgcgccac cggtcgtgac cagcgggatt ttattgcgcc
    39841 gacaataagc aatcagcgcc gctttgggcc gtacactatc aatggcatca atcacgtacg
    39901 aataacccac gctcatatac tgcgctacgt tatccggcgt cacgaaatca tccaccaccg
    39961 ttacacggca ctccgggtta atctggcgaa tacgctccgc catgacttcc gcttttgcca
    40021 gcccgacgtt atcgcgcaga gcgtgaattt gccgattggt attggtgacg cacacatcat
    40081 ccatatcgat aagcgtgatt gcgccaatcc ccgtgcgcgc cagcgcttcc gccgcccagg
    40141 aaccgacacc accgatacca accacacaaa tatgcgcgtc agcaaacagt tgcaacgctt
    40201 tttcaccata cagacgcgct gtgccaccaa aacgctgacg ccatgcatca ctaattacca
    40261 cagacatagc acctcagaat taaaaagggt gaggttatcc ctcaccctgg cgtatccgta
    40321 aaaacggtat acagaataac acatcagccg ctaaagacgt tacctgcgcc cggggcggtt
    40381 ttcagcaccc agacacgtcc atagtggttg taccaacctg cgcgatgtcc ggcttccggc
    40441 ccgatccctt gatagatatc gaagtgttgg cctttgattg cgccaccgac atccagcgcc
    40501 accatcagac gcaattcgta ctgaccgtta aatttgccgt tattatccag cagcggcact
    40561 tctgccagca aggtagtacc tggcggaata attgaacgat cggaggcaac agaagcacgg
    40621 ccaaccagcg gcaccgcact tgcccctttc accggtgcaa aagattgcgg tttaaagaaa
    40681 acgaaagacg ggttctgttc cagcagctcg cgaacctcgg cttcactgtg tgtttcgccc
    40741 caatgacgaa tcgcctgcat cgacatatct tcttttttca cttcgccacg gtcgatcagc
    40801 accttaccaa tgctgcgata ggcatgaccg tttttccctg catagctgaa aaagttaagc
    40861 ggactgccat caccaaagtc gatatacccg ctaccctgca catccataat gaagttatcc
    40921 atcagggagt tgctgtaagc gagaatatat ttatcactca gcgcccctgc gtagatctcc
    40981 gcacgagacg gcagacgacc acgttttggc ggcatacggt aaatcggata ctggaactcg
    41041 ccctggcggg tatggcgcgc ctgaattacc ggcgtgtaat agcctgtaaa ctgcacgtta
    41101 ccatagttgt cggcaccttc catctgccag gcatcaatgc cgaactggcg catattgcgg
    41161 gtatcaccgc ctgcgcgcag ccactcttgc accgcgttat aaacattact ctggttgcca
    41221 tacagacgcg gtgacgaatt acggatatgg ttaatttgct cggcaaaatc accggcgtta
    41281 atcggcgcgc caacggcatc tggctggttc accagagaga aaggctgggt aaatttcccg
    41341 tctttatatt gctgtccgcg atcggttggt ttggaagagc aggcggcaag cattgccaca
    41401 accgtgccca taagaaggta ctttacccaa cgtcctttca ttgttctctt cttcaggtta
    41461 aaaaataagg cgcaacgaag ataacaaacc gccgcgttta atgaaatgag cagcctctcc
    41521 ctgacgcaaa ttttgcacaa aaaataggct ttagtgattt gtttttgttc aaaatcatgc
    41581 caaatccgtg atcggggtaa aaaaaaggtt gcatgaaaac gcgagcggag tatagtgcgc
    41641 atccacggac gcggggtgga gcagcctggt agctcgtcgg gctcataacc cgaaggtcgt
    41701 cggttcaaat ccggcccccg caaccaatta ttgaacaccc taacgggtgt ttttttgttt
    41761 ctgctctccc ataaaaaaag cgccattcag cgccttttta tcatcccctt ctcgccagcg
    41821 tcgccccatc ggcaaaatac gctttaattc ccgcaagaat agactccgca acttcctgct
    41881 ggaaagtcgc cgttttcagt ttacgctctt cctcaacgtt actgataaac gccgtttcga
    41941 ccagaatgga gggaatatca ggcgctttca gcacggcaaa cccggcctgt tcaacctgat
    42001 ttttatgcag cttgttgatt ttaccgagct tattcagcac cgctttacca aacttaaggc
    42061 tgtcggcaat ggtcagcgac tgcaccatat cgaacatggt gtggtcgaca tagcggtcac
    42121 cgcttttgct cacgccacca atcaagtccg aggcgttctg ggtctgtgcc agatattttg
    42181 ccgcagtact ggttgcgcct ttggttgaga gcgcaaacac tgaggaaccg ctcggctggc
    42241 gactggtaaa ggcgtcggca tggatagaga caaacaggtc ggcacgctgt ttctgggctt
    42301 ttgctacgcg cacttgcaac ggaatgaaga tgtcttcatt gcgcgtcatg tacaccttca
    42361 tattgccctc tttctcgatc agagagcgca gacggcgagc tatttgcaat accacgtctt
    42421 tttcgcgcgt tttgtatttc cccaccgcac cggagtcttc gccgccgtgg ccagggtcaa
    42481 gcataataac aataggacga tcgcgccctg ctttacccgg ttgtggacca ctttgtgctg
    42541 gcggcacctg cttttcgagg tcgcctttgt tgtaatcctc cagcagcgcc agcagcgggt
    42601 cctgcatatc ctgtgcattg gccggataga ggtccatcac cagacgctct ttaaacccgg
    42661 cgaccggcgc aagggcaaac agctgcggtt ttacgttttg ctttaattca aaaaccatcc
    42721 gtacggtctg cgggtcaaat tgcccgacgc gcgccgactt gatgaacggg tcgtctgcac
    42781 gaatttgtgc cgccatcccc ttgagtaccg agttcaggtt tacatcttcg atatccacca
    42841 ccacgcgttc cgggttactc aacgcgaact gcttatattt cagctgacga tttgattcta
    42901 ccgtcacgcg ggtgtagctg gacgcaggcc agacgcgcac cgccacgacc tggctaaccg
    42961 cagccaggct gacctgactt acgctcaata gccacatagc gcccgcgcct tgcagtaaac
    43021 gacgacggct gattgcagtg ttggatcctg acatgcctct cccgagcaaa agaaatctaa
    43081 gctgtgtaac aagtaaacga ctaatttgac cggaaacttt agcgaaagac gcataatctg
    43141 tcatctaata aacggtaaac attcttttta tattcacggc attactgata aaaaagtcgc
    43201 tctcgcataa aatttacact tgcaccctgc gaaaaaacag aataaaaata caataatttc
    43261 gaataataat gcaaaggggt gtgccgtggt aaaggaacgt aaaaccgagt tggtcgaggg
    43321 attccgccat tcggttccct atatcaatac ccaccgggga aaaacgtttg tcatcatgct
    43381 cggcggtgaa gccattgagc atgagaattt ctccagtatc gttaatgata tcgggttgtt
    43441 gcacagcctc ggcatccgtc tggtggtggt ctatggcgca cgtccgcaga tcgacgcaaa
    43501 tctggctgcg catcaccacg aaccgctgta tcacaagaat atacgtgtga ccgacgccaa
    43561 aacactggaa ctggtgaagc aggctgcggg aacattgcaa ctggatatta ctgctcgcct
    43621 gtcgatgagt ctcaataaca cgccgctaca gggcgcgcat atcaacgtcg ttagtggcaa
    43681 ttttattatt gcccagccgc tgggcgtcga tgacggcgtg gattactgcc atagcgggcg
    43741 tatccggcgg attgatgaag acgcgatcca tcgtcaactg gacagcggtg caatagtgct
    43801 aatggggccg gtcgctgttt cagtcactgg cgagagcttt aatctgacct cggaagagat
    43861 tgccactcaa ctagccatca aactgaaagc tgaaaagatg attggttttt gctcttccca
    43921 gggcgttact aatgacgacg gtgatattgt ctcagaactt ttccctaacg aagcgcaagc
    43981 gcgggtagaa gcccaggaag agaaaggcga ttacaactcc ggtacggtgc gctttttgcg
    44041 tggcgcagtg aaagcctgcc gcagcggcgt gcgtcgctgt catttaatca gttatcagga
    44101 agatggcgcg ctgttgcaag agttgttctc acgcgacggt atcggtacgc agattgtgat
    44161 ggaaagcgcc gagcagattc gtcgcgcaac aatcaacgat attggcggta ttctggagtt
    44221 gattcgcccg ctggagcagc aaggtattct ggtgcgccgt tctcgcgagc agctggagat
    44281 ggaaatcgac aaattcacca ttattcagcg cgataacacg actattgcct gcgccgcgct
    44341 ctatccgttc ccggaagaga agattgggga aatggcctgt gtggcagttc acccggatta
    44401 ccgcagttca tcacggggcg aggttctgct ggaacgcatt accgctcagg cgaagcagag
    44461 cggcttaagc aaattgtttg tgctgaccac gcgcagtatt cactggttcc aggaacgtgg
    44521 atttaccccg gtggatattg atttactgcc cgagagcaaa aagcagttgt acaactacca
    44581 gcgtaaatcc aaagtgttga tggcggattt agggtaagcg atgaaaattg tcggatgcga
    44641 catgcgtaac actcgtgcgt cgcatccggc aattacgttt attcccgcga actaaacaac
    44701 gccgccagac cactgcgccg ctcagtacga gtggcgattg ccgcacttaa tatacgctca
    44761 tcggcataca gcgacagacg gcgacgcgcg cgggtcaccg cggtgtatac cagctctcgc
    44821 gttactaccg gcgtgcgttg gctcggtaaa atcaacgccg catggtcgaa ctccgatccc
    44881 tgcgatttat gtaccgtcat cgcccacgta gtttcgtgct ctggcaagcg actcggttgc
    44941 acagacttaa tattgccgtc cggcatcgca aaccagacgc gcgtcccctg cccgcgatcc
    45001 agcgcaatac cgatatcgcc attaaacaac ccaagcgcgc tgtcattacg ggcaatcatt
    45061 accggtcgac cttcgtacca acgagagtgc ggatgacgat gaattttgcg cttctgttgc
    45121 ataaactgct caattcgctc attcagtcca gccacgccaa acggcccttc ccgcagggcg
    45181 cacaaaagtt ggtactcatt gaacgcctga atgattaaat ccggctcggc acgcgcttgc
    45241 agcagatcca gataacgtcc ataacccgca agagcttcct caagcatcgc aatataatct
    45301 tctccgctct gtaaaagccg tttttcgata tcagtaaaat cctgctgaaa aacggttttc
    45361 actgccgttt tatcaccacg gttgatcgcc gcagctaact gaccaatgcc agaatcactg
    45421 ccgaaacgat agcttttttg cagcaggcag agactgtcgc gcaaagatgc cgcttctgtg
    45481 ccagttcctg ccggaacgtg agttcccgtc aggcggctta gctgcccggc acgctcggcg
    45541 gtaaagccgg cattggcata agcgcagata tcgcccagca cagccccagc ctcaaccgag
    45601 gccagttgat cacgatcgcc gagaaagatc actcgcgcat gatcgggcaa ggcgtcgatc
    45661 agtctcgaca tcataggcag atcgatcatt gacgcttcat ctaccaccag cacatcaaga
    45721 tgcagcgggt taccggcatg atgacgtaaa cgctggctac ccggctgcgc acccagcaat
    45781 cggtgcaaag tactggcatc ttccggaatg cgtttctttt gttcatcggt cagcggtaac
    45841 tgtcgcaaag ccttgccgag agattcggtt aagcgcgcgg cagctttacc cgttggtgca
    45901 gccagacgga tacggcagcg ttcgccgtcg gccatttgaa ttaacgctgc cagtaacttc
    45961 gccacggtgg tcgttttacc ggtgcctggg ccgccggaaa tcaccgagat ccgccgcgtc
    46021 agcgccactg ccgctgcaac tttttgccag ttaatttcat cgcttactgg aaaaagtttg
    46081 tccagggttt gcgccagtag agcttcatca acctcaatgg catgattcac ctcattgaag
    46141 aagcgcgcta ccgtccgctc gttacaccac atgcgattca aataaagacg atcgccacag
    46201 aggatcatcg gcgtgggttc atctcccctg ctgaccgctt gagaagccag caagcattct
    46261 tcccaatttt gtagctcacc gatttcactg acacaggtcg ccaacagcgg atgcgacgcc
    46321 tcgttatttt ccagtcgtga aagcggcaaa cagacgtgtc cctctccggc atcatgactt
    46381 aacagtgccg ccgcgagggt gacggcagga tgttcatctc ccgccacggt cagggcaaat
    46441 tgcacatcca gcgggcgtag ctgtttgtgc tccacagctt ccagtaattg cttttgcaat
    46501 ttcattacgc ctcctccagg gtcataccgg caaacatctc atccatcagg gcaatcaacc
    46561 cggcgttggg tcgggttgtg taaatccctt gttgcggatg ttctttatca acgccacgca
    46621 ggaacagata gataacgccg ccaaagtgac gatcatagtt gtaatcagca atgcgatggc
    46681 gcagataacg atgcagcgcc agggtataaa gctgatattg cagatcatag cggtgtgcct
    46741 gcatcgctgc tgccatagcc tgttgggtgt aagccgaact gtcttcaccc agccagttgg
    46801 atttatagtc gagcaggtaa taacgccctt cgtggcggaa taccaggtcg ataaagcctt
    46861 ttaacatgcc gcgtacctgc atgaactcca gcggcgggca gccagcggat agcgggtcaa
    46921 actggcgaat tagtgcatca agctggctgg cgatgagcgg ttcgctaatc ggcaggtaaa
    46981 actccatctc cacctgttta tcgcggtcgg aaagctgact caggctaaca cccgtttcat
    47041 tgagaggtgc ctggaggaca gccgtgatcc actcggtcaa caccggttcc cactgcgatt
    47101 caaagccgcc gagttccagt ttttcctgta cccagttcgg gtcaaccggc tgggtaaaat
    47161 cgaggtcttc aaacaaactg tgcaagaatg tccccggtga cgcaccgcgc gggaactgat
    47221 gcggtgttaa cgtcggttct tcaacgacgc tgaccacgcc tgcggcatca acatccagcc
    47281 gtggcatcag atcctgagcg ataccgtgac cacgctgctg caagccggag tagctggtga
    47341 cacgccagtt atcgccgggc aatcgttgta gtgtcctcgc attcagttct gcagttaaag
    47401 catcattaac ctgccagggc tggttatcac cagtttgtgc cgtttgccag gcaatatcat
    47461 catcgcataa cgcttcaata caggtgcgaa gccctgccgc atcttgcggt tccccttttt
    47521 gcagcaaacg cccgagcgca ctttggtgga cgtcggtgtc acctttttta tcgccacgac
    47581 ggcgcaccag cggtgcaacg ccgagactgc aatgccaaac cgaacgcgtc agcgccacgt
    47641 aaagcaaacg cagatcttcc gccagacgtt cgacctccgc gaggtcgacg ctttctggcg
    47701 cagcgttaag atccaaaact gcctcaaacg agtggcgatc gtgataaaac gcctgatcct
    47761 ggacgcggaa attggtgata aacggcagcc agaccaatgg atattccagc cctttcgatt
    47821 tgtggatcgt gacaatctgc accagatgtt tatcactttc gagacgcatt tgttggctgg
    47881 aggcattact gtctggctcg aggatatgtt gcgataacca gcgtaccagc gcatgttcac
    47941 tttccagctg cgttccggct tcttgtagca gttcgctgat atgcaagata tcggtaagac
    48001 gccgctcacc gcctgccgtt gccagcaagt tttcagcaat gttacgcgcc gacatcagcg
    48061 cccgcagcat cggcataacg ccacgtttgc gccagatttg ccgataacca tcgaactctt
    48121 cgactaccac atcccacgca tgttcgtcat tgttcagcgt ttcgatatcc agcgcgttca
    48181 gccccatcat tgacgttgcc agcgcactac gcagggtgtt ctcacgttcg ggcgtcatca
    48241 ccgcctgcaa caaccaaagc atttcctgcg cttccagagt ttcaaaaaca ctgtcgcggt
    48301 tcgaaaggta aacggaaggg atttccagca acgttaaggc gtcgcgcacc tgggcggcct
    48361 cctggcggct gcgcaccagc acactgatgt ccgaagcacg caccggacgc gcgtcgtcgc
    48421 cgttcatcag caacgcttcg ccccgctgtc cggcttgtag ccagtcgcgg atttgcgcag
    48481 cacatacctg cgccatggta ctttgatagt cgccaacgcc gcagctttcg ccttccatca
    48541 gccacatttt catcgcaggc tgcgtttcac ctttaaacac aaaacgtaac gcctgatttt
    48601 tcccggctga tttcactgga ataaacggta tttcgcgaaa catgaacgcg tcatcagtct
    48661 ggctgaaaag cttgttcaca ctgttcacca ttcctggtgc ggaacgccag ttggtgtcta
    48721 aagtgtagtg ggcgtgaact tcgctacgcg ccttcatata agtgaagata tccgcacccc
    48781 ggaatgcata tatggcctgt ttcgggtcgc caatcagcaa caatgcggtt tccggctgat
    48841 ggtgccagat acggcgaaaa attcggtact gctgggggtc ggtatcctga aattcatcaa
    48901 tcattgccac cgggaatcgc gtacggatcg ccgctgccaa cacctcgccg ctttcgctac
    48961 gcagcgcgga atcgagccga cttaacatgt catcaaaacc caattcgcca cggcggcgtt
    49021 tttcacgcgc tactgtttcg cggatctcag ccaatgcgcg ggttatcacc agatcacgga
    49081 tcgacaatgg ttctgcaagc agttgttcga tcgcctcaaa cagtggatgt cgcggggttt
    49141 cccccccggc tttcgtgcga tcttctaaga aacgctgaga gaatttttcc agcgactccg
    49201 gtaactgata actgttggtc tcttcttctg cccaggcgct gatcttctcg atccatttag
    49261 cctgattgct acggttaaac ttgcgtcgat caataccaga agattcgatc agcgcatcca
    49321 gttcacccac tgcgtcgcgc cactgctgtt ttaccgtatc aatacgcgcc acaatttgcg
    49381 cgtggcggga agccagcgtt tcatcatcgg gcggcggtgc tttgataacc ggtgcttcgc
    49441 cttgcagata acgattaata tcgcgcagca acgcctgcgg ccctttccag gtttcaaaga
    49501 ccaccagggc aatttcacgc ggcagcgggt agcagtggcg acgccagaaa tcggcgcagg
    49561 cctggtagcg tagcagagac tcatcttcaa tcagctgctg ctcaaacagc ataccggatt
    49621 caaaggcatt caggttgagc atgcgctggc aaaaaccgtg aatagtaaag actgccgctt
    49681 catccatctg ccgttcggct aacaacaacc actgcgcggc ttgcgcttta tcgtcgatct
    49741 cttccagcag gcgttcgtac agtggattgt cggtggtttc acgcagacag gcgatgcgca
    49801 actcgtggat attgctacgg atacgaccgc gcaattctgc cgtggcagcc tcggtaaagg
    49861 tgaccaccag cagttcttca acggtcagcg ggcggggaaa ggcggcggaa ccgcctagtc
    49921 caagtaacag gcgcaaatag agcgccgcaa tcgtaaaggt tttgcctgtg ccggcagagg
    49981 cttcaatcag gcgctcgccc tgtaagggca agcgcaaagg atctagtgtc tcggcgacat
    50041 cactcattct tttcactcat caggggcatt gtttgctgca acgcgctgac gttctcccac
    50101 actttccagc cttcaggatg cacatattcg gctttcccgt tctggctgcc ggaaatctgc
    50161 gacaggatag ccatgccttg cggctcgacc accgcctgat ggaagaaatc agcaagtttt
    50221 tgcggcgtca gcagttttat ctgggccacg attttatcac gcgaatcgaa gcgcatattg
    50281 ccgcgatcga aatctttact taacttcgat gcttcttcgc cgagcgtttg cggtgcctgc
    50341 agcatctggg taattaccgc ctgctggatt tgcgcaaact catctggctt catcgctcgc
    50401 aattttgcct ctgcggttgg gaaaaacgcc ttgtaacgct cccacaagaa tgaaggctgt
    50461 ttatcattgc tttgcaaaag gaagcccatg ccccactgac gccccacgct cattggaaac
    50521 gcaaacacgg catagcccaa ttgttcttcg gtacgcaact gattgtagaa ccacggctgt
    50581 acgatctgcc ccaacagaga gctataggct gagctggtgt attcatcgta gccagtcggt
    50641 acaaataccg ctgccagtgc ggagtcggtg ctgttaccgg ctttttcaaa gatgacggat
    50701 tgttttttat cgaccactac atctttgttt cgacaccact ctgaaccatc agcgcccaac
    50761 tgtttttgca catcgcgtgc cagcgttgtt gcctgggcct cggtcatgtt gccgataacc
    50821 ataaactctg gtcgagcccc tgattttaag gcgtcgcgat aggccagcac ctctttcaac
    50881 gtaatggagg gcaaaatttt acgccgttca tctcgcgaga agtacggcac ttgcgagagc
    50941 atctgtgcgg gcataatcgc ctgctcaaac gctttaccct tttctgcgga atccatcatc
    51001 tggttatacc aggacttcgc ctgctcaagc tgatcttccg tagcggtata gctaaagtac
    51061 ccctcgagca atgcctggaa cagctgcggc agacgctggg tgtaaccatt agcattaacc
    51121 ataaggccgt tgttagcgtt ggtggaaaaa cttatgccac caaccgacgc ctggttgctt
    51181 aactgatcaa gcgccagccc tgcgagataa tcattgagcg caaacatcac ctgattgcgg
    51241 gcgctgtcca tggctttcgg attacgcaaa atcaggctga catcagcttt gggttcgctg
    51301 gaaaaataac ggcttggcgc atacaccacg cgcagattcg actcatcaac aatcagctct
    51361 ggatggtcat atttcttctc tgacttaatc agcgagaaat cgtcaggaat ataggggtta
    51421 agctccggta aggagagcgc aatattggca gctttttgct gccagtcagc gaaagtttgt
    51481 tcgctgattt tatcgacctg atacggcgca tcgacaaaat aagccgtttt attgtgcggc
    51541 tctttcgggc tgatatacca gatacgcgca ttctgcggcg tcatcatcgc cagacgttct
    51601 tttactgctt tagcatcgta ccgatcggca atattgactg catccagcgt atgctcaacc
    51661 ggaacgcgaa tcatggtatc tgccagccat tcgacgtaat ccatatcacg ggtgatcgac
    51721 ggataacgga agtcgatatc cagcacattc gccagttcat cgaagtattg tttatcaatg
    51781 cctttttcac gtaacagatt gagatagcta aaaattgccg ccacaacctg atcgcgatta
    51841 gccaggcctt tatcggttaa agacgcagag atcgctaata cgccgctgtt gccgttgacg
    51901 ataggatcgg agttggcgct aatgccctca actaatccct gcttttgcag ccagtcggaa
    51961 agtgtacctg ggctgcgatt accaatcaga taggtaatca attcatccgt tttactacgg
    52021 aacttcgctg agttgttatc gatgcgaaac tcaacgcgca gaactttacg cggcagtgcc
    52081 gggacgtaat gaatgataat gcccttttgc gcgtcggtga ctaccggcac ggtgatttcc
    52141 ggttttttgc tctctttgtt cggcacgcga ccaaaggtgt ccgccgccat ttttgccaac
    52201 tccggcagcg gtttattgct gtaaataacc gccttcatca gattggcgga atagtacttc
    52261 tcgtggaaat ctttcagcgc ctgctgcacc ggattaccag gtttatcgct taaagtttcg
    52321 aggttaccac cagaaaactt tgaaccgggg tgtgccgggt taatggtttc tgcgctgacc
    52381 tgtgccatgc gcatcccgtc acgcgtacgc gccatggtta attcagcgtt caccgcatta
    52441 cgctcacgtt cggcatattt cttgtcgagc aaaggttcag caatagcatc ggccaggcgg
    52501 tctaccgcac caggcaaggc gtcgttctca acttccagat agaaagccgt gcgatacggc
    52561 gcagtgctgg cattgtgact accgccgtgc attttgagat attcggccag actgtcagcc
    52621 tgcgggtact ttttcgaccc catcagactc atatgttcaa ggtaatgtgc cagcccctgg
    52681 tacgcctcgg gatcttccag cgacccaacg ggcaccacca gcgccgagag cgatttaact
    52741 gcctgcggat cagaaaccag caagaccacc ataccgttat ccagacgtat agcctgatac
    52801 tggcggttat ctttatcact tttacggatg gtttcctgaa tcggctgcca tcccgtttct
    52861 gcctgactta agggtgccca aagggcaaca aacaacaata atgctttgaa ccaggtgctg
    52921 cggggcattc acggacctca taatcaactt tattttctgg ccagattcaa caggttaacg
    52981 caaatcatct gccagaattt aatcttgtgc tgcacgagtc agcctatgtc tatatatcca
    53041 tcagtccgtg actggtgcgc atcataaagt aagcggatag attgcgcaat ttttatacag
    53101 cactcatgac tgattaaagc gaaacagcgg taacaggaaa cgttgcgact gttcaacgat
    53161 ggcctccatt gtctctggtg ttaattgccg ccagagcctt tgataccaga tatcatcacc
    53221 ttcgccacgc accatcatgt tgccttcgta agcctgaagg aatttcgtac gggctttttg
    53281 caacgtggaa tcgtcatcca gcatggcatc gttttgcgcg tcataacagg tttttagcca
    53341 cgcgccgcca ctttcaggta acaccagcaa tggcgcggac atcccttcac gatacccctc
    53401 aatcagttgt gagaggtaat gcaaagcctg ttcggctgca agcggcggaa aacgccactc
    53461 gccgtctttg cgtagaaaaa ggcgactttc accattacca ccgctggcac agtagacaag
    53521 gtgttccagc caaagttgca ttccctgcgc cacacttaat aaagagggac gccagcgcaa
    53581 caggccatcc ggctgcacct gcggcaacca gccagttatc tgcacaccgt tgcaggcgag
    53641 atcaatttcc atactctgcc ccggctggcg acaggcaatg actctgtcgg caagctgctg
    53701 catctcctgg cactgtgttt cccagaaaat ttcaccaaaa gcgccatacg gtaaatcccc
    53761 tgccgcccgg aagcggcgga acaagcgttc ggcatcatcc tgctcaacca gtgcattcaa
    53821 taactgctga ttgatttgat aacggctaag cccttccaga ataaatggct cggtgtctgg
    53881 gatttcgctg tcttcagtac ggaagttcac ctgcaaacgc atctggaaga acgcccgcac
    53941 cggatgtgcc cagaatcgtt gtagcgtttc cagcggcacg gtttccggta aggtaaacgg
    54001 cagcggctga acaaattcag aatgtgcttt accagcctgg ctggccgcag gtagccattc
    54061 acgagcatag ctttgtcgtt cgcctggctg gtagttttgc ggatcaaacg gcatccgggt
    54121 atggaggcaa gtaagatgcg cttttaccct tgcctcgctt tcatcacagt tgagcgcttc
    54181 atcgcccggt agataatgac tttgcccgat gtagtcgatc agttcctgca ccagtaccga
    54241 cgggaaacgt tcactgttat cctgaatgga acgcccgata tagctgatat agagtttttg
    54301 ctgcgcggaa attaacgctt ccaggaacag gtagcggtcg tcatcgcgac ggctacggtc
    54361 gccacgcttc ggtttctggc tcatcaggtc aaagcccaat ggcgcaagct gacgtggata
    54421 aacgccgtcg ttcattccca gcaggcaaac cactttgaac ggaattgaac gcattggcat
    54481 cagagtacaa atgttaaccg gtccggcgag aaaacgctgg ctgatacgtt cttgatccag
    54541 acgctgcgcc agttcatcac gcaatagtga cagcggcacc gcgtcgccat actgcgcacc
    54601 taaaccttcg gcgataatcg cctgccattg ttgttcgatc agcgtcatcg ccgcttcggt
    54661 ttccgcatcc ggcaggaaga aggcgttgag catatcgcga caaaccggca accactcttc
    54721 tagcggacgc tcctgtgcca gcccgcgacg ccagatgttt agctgcatta gcagtgaagc
    54781 cagatgcccc accagttctg caattaagcc gctcgattca tcataaggta gaaccgattg
    54841 ccactcgccc tgcgcgctct ccatcgcgta gcccagcaac atacgcgtca ggccaaatcg
    54901 ccaggtgtgt tgtccggtgg tggggagttc cagctcgcga acgttgtcgt catctatccc
    54961 ccaacgaatg ccggattcgt tgacccactg gcgtaaataa cgcagccctt cttcggtgat
    55021 gtcaaaccgc gccgccagca ccggcacatc cagtaatgcc agcacgtctt ccgacacaaa
    55081 gcggctgtct ggcaacgata acaggctgat aaacgcttcc agtaccggat gcgactgccg
    55141 cgcccgacgg tcggaaatgg cgtaaggcag gtaacgatcc gcaggtgcgc taccaaacac
    55201 agcctgaata aacggactgt agctgtcgat atcagccacc atcacgatga tgtcgcgcgg
    55261 agtaagtgtc gggtcttctt ccagcatcgc cagcaggcga tcgtgcaaaa cttcaacttc
    55321 acgctgcggg ctatggcaaa cgtggaaggt gatactgcta tccagtggat caagcgggcg
    55381 tttgttatcg ctacgggaaa actcttcgat gttcacacca gcaacggcac ggttttccag
    55441 ttccagaatg tcagactgaa tgttatgcag caggttatct ggcgtgacat cgacaaaggc
    55501 gtccagctcc tggctgctct ccaggtcaga aaggagataa atgtagtcgc gcccaagctt
    55561 accccatgaa gccagcagcg ggttgccgac atcctgttca ccatcgctgt taaagagctg
    55621 cccggcattt tcgctatcac gaaatagcgg caattcgcga tcttcaaaac tgtgtcgacg
    55681 ctgacgggtc agcagtttcg ccagataagc aggatcttta atatcgcccc agtaataacg
    55741 gcaggggttg gtaaacagga gatggatttc aatatgttta cccagcgcct ggagcgcctg
    55801 gagataaaca ggcggtaacg cggaaatacc gcatataaag acgcgcgaag gtaaccccgg
    55861 cgggcaggtc gtcgcggact ccagcgtttc gataaagcgc tgatagagat tggcgcggtg
    55921 ccaacgcggt tgtccgagtt catgggtata ttccaccagc gccttccaca acggcgcttg
    55981 ccaggcctgt gcttctccca gcccttcaac caaatgtcct gtttcccact gtgccagcca
    56041 ctccgggcga tagaccagat actggtcaaa caggtccgcc gcttttgagg aaagctggaa
    56101 cagttttcgc ttgtcgctat cgtcagtcag ataatgccgc aacagggtaa agtcttcgcg
    56161 atccagcaat tgcggcagca gagtcatcag tttccagctc atgctctgtt tgttaaaggc
    56221 gctctctttg gggatctccg gtaacacccg gacgaacata tcccagataa agctcgctgg
    56281 cagcggaaaa tcaatgtttg ccgcaatacc aaacttttgc gacagggtca tttgcagcca
    56341 ctgcgccata ccggtacttt gcaccagaat catctctggt tcgaaaggat cgtccagccg
    56401 ttcgcgttcg acaataaact ccatcaacgc ttccagcacg tccagacgat tggaatggta
    56461 gacccttaac atagcggctc ctgactactg acgattcggg caatgcaggc gggtcatctc
    56521 gccttctctg cccccgggtg aaactagcgt aacgctgatg ctgacacatc ccgcctgcga
    56581 tgtctgcatt cggttgacct gccagttggc aggtggcgaa atcgcgcgca gttgcgtttg
    56641 ctgccaacca tgccgccaga gctgttggta ctggtttctg ctggcgagac tgttcattaa
    56701 tgttcgctgg atacccgata acgcagtgac aatcatcacc atcagcacca tcgcaaccat
    56761 tacctccggc aggctaaagc cttgttgatt cttcagggaa gctgacataa cgccctctct
    56821 ttcagcggac aaaaatcgct ccagccgcgt ggcgaaaaaa caatgtttcc atcaatgact
    56881 tcgcctgttc gccacaacga aacgccttca taaccggcaa ccaataaggc ttcattatct
    56941 gccagtaaac gcaaacaaac ccgggcatcg gttccagcgt actgcgagca ctgaactgcg
    57001 ggctgcgtct gccaggagtg cattttcccc cacgccagcg ccgactgaac gatggcctgg
    57061 cggcgcaatg actgactttc catgctcacg cgagaggcaa aactgcgatc ctgctgactc
    57121 attccttgca atagcaagct acccaaaacc agcagcatca gaaccagagc cagtgacgaa
    57181 acaccttttt cgcggttcac aggttgaatc ctgtcacgct atagctggca tccaccacgg
    57241 tttgcggttc agacttactg gcagcacgca tattaaccgt caacaccggc gagaagccgc
    57301 tgacatcctg acgtacgacc tgaaaagtgt cgataatgat ggcatccgga ttagtgactt
    57361 tatcccagcc cttaccttca caggatgtcg caccgcgtag cgtttccagc acatgctcct
    57421 tcagacgaaa tccaatctgg tcagactctt ttaccggttc gcgatcccag ataccgttac
    57481 tgttcgcatc ccactgcaca atgatacagt caccctgtcc gacaatttcc agcccttcgc
    57541 cggtacagct gccatgacaa taacccgccc tctggagatg cttcgcgacg gtaaataccc
    57601 gcagccagat ttcatcttcc agcgccagct tacgggtgct cgttaaactt tcacgctgta
    57661 acgcaggcag aaagcgtgca gcccccagca acaatacgct actgatcgcc atagcaatca
    57721 acacttccag cagagaaaaa ccttgctctt ttacaggcat ccttctgttt ctccttgctg
    57781 acaaagccgg agtcttcccc acggcgaaac caccagccac cactcgcccg ttgagttttt
    57841 gaagcgaata tgcccggccc atgcggtatt gcgcaggcca aagaaagcaa gcgaaggtgt
    57901 caggtcgctc atttcgactt cgggccagcg tggcacaaag accaatggtg aactgccatg
    57961 acaggtattg gccccagcag cggaactcac aaggcaccat aacgtcccct ccctgataac
    58021 gctgatactg tggtcgcggt tatgccagtt ggcatcttca cgtaaataga gcaaatagtc
    58081 ccgcgcctgg ctggcggttt gccatagccg ttgcgactgc tgccagtatt gccagccata
    58141 gagtccactt gcgcttagca tgaccaaaat cagcatcgcg accagcgttt caatcagcgt
    58201 ataaccacgt tgtgttttca tgccggcagt atggagcgag gagaaaaaaa gacgagggcc
    58261 agtttctatt tcttcggcgc atcttccgga ctatttacgc cgttgcagga cgttgtaaaa
    58321 tttcgggaag gcgtctcgaa gaatttaacg gagggtaaaa aaaaccgacg cacactggcg
    58381 tcggctctgg caggatgttt cgtaattaga tagccaccgg cgctttaatg cccggatgtg
    58441 gatcgtagcc ttcaatctca aagtcttcga aacggtagtc gaagatggat tcgggtttac
    58501 gtttgataat caacttcggc agcggacgcg gttcgcggct taattgcaga tgagtttgat
    58561 ccatatgatt gctgtacagg tgcgtgtcgc cccccgtcca gacaaaatcg cccacttcca
    58621 gatcacactg ctgcgccatc atatgcacca gtaacgcgta gctggcaatg ttaaacggca
    58681 ggccgaggaa aacgtcacag gagcgctgat aaagctggca agagagtttg ccgtctgcca
    58741 catagaactg gaagaacgca tggcacggtg ccagcgccat tttatccagt tcgcctacgt
    58801 tccacgctga aacaataatg cggcgcgaat ccgggtcgtt tttcagctgg ttcagtaccg
    58861 tagtgatctg gtcaatatga cgaccatctg gcgttggcca ggcgcgccac tgtttaccat
    58921 acactggtcc gaggtcgccg ttttcatcgg cccattcgtc ccagatggtg acattgtttt
    58981 cgtgtagata agcaatgtta gtgtcgccct gcagaaacca cagcagttcg tggatgatgg
    59041 aacgcaggtg gcaacgttta gttgtcacca gcgggaatcc atcctgcagg ttaaaacgca
    59101 tctgatgacc aaaaatggaa agcgttccgg ttccggtacg gtcgtttttc tgtgtgcctt
    59161 cgtcgagcac tttttgcatc agttctaaat actgtttcat ggttcctcag gaaacgtgtt
    59221 gctgtgggct gcgacgatat gcccagacca tcatgatcac acccgcgaca atcatcggga
    59281 tggaaagaat ttgccccatg ctgatgtact gcacccaggc accggtaaac tgcgcgtcgg
    59341 gctggcggaa aaactcaaca atgatgcgaa acgcgccgta gccaatcagg aacaagccag
    59401 agacagctcc cattgggcgc ggtttacgaa tatacaggtt gaggataata aacagcacca
    59461 caccttccag cagcagctcg taaagctgtg atgggtggcg cggcagcaca ccgtaagtgt
    59521 cgaaaatgga ttgccactgc gggttggttt gcagcagcaa aatatcttct gtacgggagc
    59581 ctgggaacag catggcaaac gggaagttcg ggtcaacgcg gccccacaat tcaccgttaa
    59641 taaagttgcc cagacgcccg gcaccaagac caaacggaat gagtggtgcg ataaaatcag
    59701 aaacctggaa gaaggaacgt ttagtacggc gggcaaagat aatcatcacc acgataacgc
    59761 caatcaggcc gccgtggaaa gacatgccgc cgtcccagac acggaacaga tacagcggat
    59821 cggccataaa ctgcgggaaa ttgtagaaca gaacataacc aatacgtccc ccgaggaaga
    59881 cgccgaggaa gcccgcatag agtaagtttt caacttcatt tttggtccag ccgctgcccg
    59941 gacgattcgc ccgtcgtgtt gccagccaca ttgcaaaaat gaaacccacc agatacatca
    60001 ggccgtacca gtgaagcgcc acgggtccta ttgagaaaat gaccggatca aactccggaa
    60061 aatgcagata gctactggtc atctgtcacc acaagttctt gttatttcgc tgaaagagaa
    60121 cagcgattga aatgcgcgcc gcaggtttca ggcgctccaa aggtgcgaat aatagcacaa
    60181 ggggacctgg ctggttgccg gataccgtta aaagatatgt atatgatccg cgctataacc
    60241 ctccgcgaat cagcccgccc atgccgcgac gctccataaa ggctgcaacc tgatggcgaa
    60301 cttcggtcgc cagttgcgct tccagactac gctgcgcaag attttctgct tcggcataat
    60361 caatgcgccg cagcaggtat tttacccgcg ctacagaacg tccgttcata gacagatggc
    60421 gatacccaag cccaatgagg attgccacgc acatgggatc gcccgccatt tcaccgcaca
    60481 aacggagatc gattccatgt atttccgctt cccgggcgat catcgccaga gctcgtaaca
    60541 ttgcaggatg aagactgtca taaatgttcg ccacccgggt attgttgcga tcaacggcca
    60601 gaatgtattg agtcagatcg ttggtgccaa cagagatgaa atcgacccgc tttgccagat
    60661 gcggcagcat aaataccatt gacggcactt ccagcatgat gccgatacgt ggtttgggaa
    60721 tttcgtaacc gatcatctcc tcgacttcac gtccggcacg ttcaatcagg cggcgtgctt
    60781 catcaacttc atcgaggctt gtgaccatcg gcaacagaat attcaggttg cccgtagcgg
    60841 cattagcacg cagcatcgcc cgcacctgga tcaagaagat ctccggctga tcgagcgtaa
    60901 tgcgaatccc acgccaaccc aggcatggat tctcttcgct gatcggcatg taaggcagct
    60961 gcttatctgc tccgacatcc agcgtacgca aggtgacggg tttatcattg aacatttgca
    61021 gcatcccctg atactgcgcc acctgttctt cttccgacgg gaaaccactt tgcagcatga
    61081 atgggatttc agtgcgataa agaccgatgc catcaatacg gctgcccagt ttttcttcat
    61141 gttccgggct taaaccagca ttgagcatga cttttatacg ctcaccgctt tttaactgcg
    61201 cgggtaaatt gacgtcatct tccgccagac ggctaagctc aatctcttca ctaattagcc
    61261 gctgatattc ttgcagcagt accggctccg gatcgaccag caattcaccg cgatagccat
    61321 caacgatcag cgtcctacga tgcagcaccg aaggctgaat atccgcgccc atcacggtag
    61381 ggatccccag cgcacgtacc atgatcgcag catgggagtt ggctgcgcca tctcgcacga
    61441 caacaccgac taagcgatcc tggggcagct cagcaagagt tgtcgctgac agttcatctg
    61501 ccaccagaat gaaacgttcc ggccaggcgt tcggcccttg attagcgtca tcaagatgaa
    61561 acagcaatcg ctgacccagc gcacgtaaat cgccagcccg ctctttgaga tagttatcgc
    61621 ttagcgcggc aaactgttcg gcaaattttt caatgaccgt ttttaccgcc cactctgcca
    61681 ccgagccttt atcaacctcg gcaaacaatt cgcgacgcag ccgggtgtcc gaaagcaggt
    61741 gcgagtaaag atcgaaaata gccgccgttt ctttttgtgc accggcggca aagcgtttgc
    61801 tgtagcggcg aaactcgttt gccgcttctt ccagcgcccc ggtcagtcgt tcgcgttcca
    61861 gagccggatc cagcgttgat gcctgataca cctgttccat taaaggtaac gtggcatcct
    61921 gccagccttc ggcaatcgcc acaccaggtg ctgccggtaa tgcgcggatt cgcgtctggc
    61981 gatattgccc aaacagggca gtcaactgcg actgagaaag aatagctgcc atctgggtgg
    62041 caagcgtcac caggaaggat tcttcacttt cgtcatactg gcgcaactct cgttgctgta
    62101 ccaccagtac accaagcaac tggcgacgtt gaataattgg tacgcctaaa aacgcgcgga
    62161 aacgttcttc ttttacggag gggatgtatt tgaagctggg gtgcttttgc gcatctgcaa
    62221 ggtttatcgg ttccgccagc ctgccaacca ggccgacgat cccttcatca aacgcgagcg
    62281 ttacagtgcg accgcgtggt tttttcagcc cccgggtcgc catcaggtag taacaacgtc
    62341 gatcatgatc ggccaggtag accgaacaga cctcggtatc catcgcaaga cagatgtcgg
    62401 taaccagaat atttaacgcc tcattcaggc gtggtgcgct ggctaccttt tcgactattt
    62461 cgcgcaggcg agtgagcata attggcgtga cttaacctct tttacgtcga taagcagatg
    62521 cgttttgtgg ttttggcgta ttttcctgca gtgacatcac cacactcgcg aactctttca
    62581 ttaccctacg gtagacatca cgtttaaatg acaccacctg tctgaccgga taccagtaac
    62641 ttacccatcg ccagccgtca aactctggtg tactgctggt ttgcatattg atttctgcat
    62701 cgccgctcac cagctgcaag agaaaccatt tttgtttttg gccgatacaa accggcttcg
    62761 tgtcccaacg caccaaacgt ttcggtaatt tgtagcgcaa ccagttacgc gttgaagcaa
    62821 ggattcgaac gtctttgcgg cttaatccta cttcttcaaa caattcacgg tacatcgcct
    62881 gctctgcgga ttctccgggg ttgattccgc cttgcggaaa ttgccaggag tgctgaccaa
    62941 atcgccgggc ccacattacc tgcccctggc gattacaaat cacgatacct acgtttgggc
    63001 ggtagccatc gtcatcaatc accggactac ctcaaaataa agctttatat acgaatgatt
    63061 gtttcatact ccaggaagac ggtaaaccac tctctgcagg gcattacaca ctaataacaa
    63121 ttgaataact cacagttatg tgcagagtta taaacagagg aaggggtgga tagccgtttt
    63181 tattcacctt ttctgtggat agagttgtga agaactacgg aattactacg ggaaaacccg
    63241 gagcattctg aataagcccg atatagacat tttaaaatat tcattattag tcaatgcatt
    63301 acatgtaatt tgagtaatga aaaagagtaa ttcgtgaccc aggtcacacc tctcatttac
    63361 gggttggcga aagatcaacc aatgccgtat ttatccacag aatgtgccac taagttaagc
    63421 actgaaccac taaaaactgg agtttcatcg cacgtcaagg ctgtaaatgg aaacagtagt
    63481 ggaggttttt cacagttatc ccagctttct gtggataaca tggtgtaaga tcctgtttat
    63541 tttcagtgac cagatttgga aaacctattg cagtgttgcg caactcgatt accggcagcc
    63601 taaaaagcaa tataaatcag ggaattgaac aacacatgtg gaaaaagtta cactgcgaat
    63661 attcggcaca taattgctgt ttgtttttta atcaaggtat catgacatgt cccaacctcg
    63721 cccactgctc tctcctcccg aaactgaaga acagttgtta gcgcaagcac agcaactttc
    63781 tggttataca ttgggagaac tggcggcact tgccgggctt gttacgccag agaatttaaa
    63841 acgcgataag ggctggattg gcgtgttact ggagatctgg ctaggtgcca gcgcagggag
    63901 taaacctgag caagattttg ccgctctggg cgtggaactt aaaactatcc ctgtggatag
    63961 tcttggtcgt ccgctggaaa caacattcgt ttgtgttgcc ccgttaacgg gtaatagcgg
    64021 ggtgacctgg gaaaccagcc atgtgcgcca caagctcaaa cgcgtactgt ggataccggt
    64081 tgaaggcgag cgcagcatcc cgctggcgca gcgtcgcgta ggatcaccgt tgctgtggag
    64141 cccgaatgaa gaggaagacc gacagctacg cgaagactgg gaagaattaa tggatatgat
    64201 tgttctcggt caggttgagc ggatcaccgc tcgtcacggg gaatatttac agataagacc
    64261 gaaagcagcg aatgcgaaag cgcttaccga agccattggt gcccggggcg aacggattct
    64321 gacgctgcca cgcggctttt atttgaagaa gaatttcacc agtgcactac tggcccgtca
    64381 ttttctgatc cagtagccat cgctttgacc tgccgctttc cgggcatata attaccgctt
    64441 catttttttg gcagggcttt ttagatgtta tttgcatgga taaccgatcc taacgcctgg
    64501 cttgcgctcg gtacgctgac gctgctggag atcgttcttg ggatcgacaa tattattttc
    64561 ctttctctgg tggtggcaaa gcttcccaca gcacaacgtg ctcatgcgcg ccgtctgggg
    64621 ttggcgggag ccatggttat gcgtctggcg ctgctggcat caatcgcctg ggttacgcgc
    64681 ctgacaaatc cgctttttac aatattcagt caggaaattt ctgcccgtga tttgattctg
    64741 cttctgggtg gcttgttcct tatctggaaa gccagcaagg aaatccacga atccattgaa
    64801 ggtgaagaag aagggctgaa aacacgcgtt tcatcattcc tcggcgctat cgtgcagatt
    64861 atgctgctgg atattatctt tagcctcgac tcggtgatta cggctgtggg tctgtcagat
    64921 cacctgttta ttatgatggc agccgtggtg attgccgtag gcgtgatgat gttcgccgcg
    64981 cgctcgattg gtgattttgt cgaacgccat ccttcggtaa aaatgctggc gctctctttc
    65041 ctgattctgg tgggctttac cctgattctg gaaagtttcg acatccacgt accgaaaggt
    65101 tacatctact tcgcgatgtt cttctctatt gcggttgaaa gcctcaactt gattcgcaac
    65161 aaaaagaatc cgctctgata ctccgtacgc tctcctgcgg gagggcgtac tcactccttt
    65221 taccctcaca gtttaagatt ttcctgcttt caagatatat aacgtcggtt tataaacaga
    65281 ctattatcat aggtaggcca aatgaggata accggatgaa aaaatgggca gtaataattt
    65341 ctgcagtcgg actggcgttt gctgtttccg ggtgttccag tgattacgtc atggcgacca
    65401 aagatggccg tatgattttg accgatggaa aacctgaaat tgatgatgat accgggctgg
    65461 tgagttatca cgatcagcaa ggtaacgcga tgcaaattaa ccgtgatgat gtttcgcaaa
    65521 ttattgaacg ttaacaaata aggtcagcat ccggctggcc ttaagatttt tctctttccc
    65581 ttttcccttc cctctgccat ttttatattc cttatgtcgt gattataaaa aggaaacggc
    65641 tatgcaatat caccgtatac cccacagttc gctggaagtc agcacgctgg ggcttggcac
    65701 gatgacgttt ggtgaacaga acagcgaagc cgacgcccac gcacaactcg actatgccgt
    65761 cgctcagggc attaacctta tcgacgttgc cgaaatgtac ccagtacctc cgcgccccga
    65821 aacgcaaggg ttaaccgaaa cctacgtcgg caactggctg gcgaaacatg gcagccgcga
    65881 aaagttaatt atcgcctcca aagtgagcgg accgtcgcgc aataatgaca agggcatccg
    65941 cccggatcag gcgctggacc ggaagaatat ccgcgaagcg ctgcatgaca gcctcaagcg
    66001 cctacagact gattacctcg atctttatca ggtgcactgg ccgcagcgcc cgaccaactg
    66061 cttcggcaaa ctcggttata gctggacgga ttctgcgcct gcggtttcgc tgctggatac
    66121 gctggacgca ctggcagagt accaacgcgc gggaaaaatt cgttatatcg gcgtgtcgaa
    66181 cgaaactgca tttggcgtaa tgcgctacct gcatctggcg gacaaacacg atctgccgcg
    66241 tattgtcacc attcagaacc cttacagtct gttaaaccgc agttttgaag taggtctggc
    66301 agaagtcagc cagtatgaag gggtcgaact gctggcctat tcgtgcctgg gtttcggcac
    66361 gctgaccggg aaatatctca atggtgcaaa acccgctggc gcacgtaata cgctctttag
    66421 tcggttcacc cgctatagcg gtgagcaaac gcaaaaagcc gtcgcggcgt atgttgatat
    66481 cgccagacgt catggcctgg accctgctca gatggcgctc gcgtttgtac gccgtcaacc
    66541 gtttgttgcc agcactctgc tgggcgcaac cacgatggat cagctgaaaa ctaacatcga
    66601 aagtttgcat ctggagttaa gcgaagacgt attagctgaa attgaagcgg tgcatcaggt
    66661 ttatacttat ccggcaccat aagattaacg ccggttaaaa ccggcgttaa atattaatga
    66721 cggcgctgcc agatccacag cgccgttatt gccagcgcaa acagcgcacc gaagccaatg
    66781 ccaatgggca cgaccgggat gcctatcatt accgccagcg agtaaatgcc cagcatcaac
    66841 aacatggcgc tgttttcgcc aaggttttgt actgcaatcg cattccccgc cccgacgctt
    66901 tttttacccc gctcctgtag caacgcattg agcggaacga caaaaaagcc ccccatcacg
    66961 ccaatcagca tcaacaaggc ataggctggc agcagctcgt gttgcaggga aaaaatcagt
    67021 accaccacgc caatcaaaat cccggctggc atacagcgtg acacggtttc cagcgtaact
    67081 aacttcgctg ccgcacctgc gccaaccacg ataccaatcg ctaccatcgc gttgagatag
    67141 gtgggcgtag cgttatcggt aatgcccagc gccaccggta cccacagcac caacaggaaa
    67201 cgcagcgtga cacccgctcc ccagaataaa ctggtgccca ccagcgaaaa acgcgtttca
    67261 ccattgcgcc atagcgaggt gcaggcattc aggaaactgc gggtcatgtt gatgagattc
    67321 caggactgcc ccggacgcgc cgccgccagt ttgggaatgt agatattggc aacgaccgca
    67381 ccaccgtagg ccagtgcgca tgcggccagg gcgacgagga catgccagtc agccagcaca
    67441 ccaccggcta cggaaccgag caaaatcgcc gctatggtag aagcttccat taaaccgtta
    67501 gctttcacta acttactacc cgtggttaat tcgccgagaa taccgtattt cgccggtgaa
    67561 taggctgcag caccaacacc caccagcgta tagccgagaa acggattgat accaaagcag
    67621 atactggctg cgcccagcag cttcaggccg ttggcaaaca tcatcacccg gcctttggcg
    67681 aagctatccg ccacctgccc gacaaacggc gcaaaaagaa tgtaagcacc tacaaacacc
    67741 atttgcagga tgggctggct ccactccgga tagaactgcg ctttcagtaa cgccagagtg
    67801 gcaaacagta gggcattatc gccaaacgca gagagaaact gcgccacgat aaccgctttc
    67861 atccccttcg accacaacga agtgttagtg tgcactgact cactcatcgt gttgttccgc
    67921 ttcgtctacc cagcttttca acgtgacaaa gtcaggtttg ccgctgccaa gtaatggcat
    67981 ctgtttcaga tagcgaatat cgcgcggtac agcaagctcc ggcacgccgt gctcgcgggc
    68041 atactgttgc aacttatcgc gcgtcagttc gttatctgtg gtgaaaagca ccagtgcctc
    68101 gcctttgctg gcatcgctct taatcgcagt ggcatggact ttatctggcg aaacaccaag
    68161 tgccagttgt tccaccattt ccagcgacac catttcgcct gcaattttgg caaagcgttt
    68221 tgcgcggccc tgaatctgca caaagcccag ctcgtcaaaa cgcacaatat cgccagtgtc
    68281 ataccagccg cgctccattt cgccgcgaac attctcggcg gtgggcactt cctgtacacc
    68341 tggcttctcc acccgcagat agccgttcat tatgttcggc cctttcagtt gcaggcgtcc
    68401 gccctcttcg ataccaggga ccgacaacag gcgcgcatcc attcctggta gaatacgccc
    68461 taccgtaccg ggtttcgccg ccatcggtac gttgatagaa acgacaggcg cgcattcggt
    68521 cacaccgtag ccttcaagga tgcgcaggcc aaatttatcc tgccaaagct gtttggtact
    68581 ttcttgtagt ttttctgcgc ctgccaccac atagcgtaga cgatagaagt catacgggtt
    68641 ggcgaaacgc gcgtagtgac cgaggaaagt cgaagtgccg aacaacacgg tgcaactgcg
    68701 gtcatacacc aactccggca caatacggta atgcagcggg cttggataaa gaaacacttc
    68761 tgcacctgtg agcagtggcg taaacaggcc taccgtcagc ccaaaggagt gaaacagcgg
    68821 taacgccgac ataaagcgat cgttggtggt gaagtcggca atcgttttaa tctgctcgac
    68881 attcgccaga atgcttttat ggctatggac gacgcctttc ggatggcctt cagaaccgga
    68941 ggtaaaaagg atcagcgcct cttcttccgg ctgctgttta acctgtgcca gacgcggcat
    69001 cagcaaatga gcgaagatcc atactttgtc ggcagtggtg acatctgctt ttaaatcttc
    69061 cagatagacc cagcgcacct gagtaagttg ctccggcaga tgccagagtt tgcctttatc
    69121 gagaaactgg cgggaagtga agatggtttt gatttcagcc gccgtaatag cactggtcag
    69181 cccttttacc ccggcagtgt agttcatcat tgcgggaatg cggcgacggg cgatggcccc
    69241 aaaaatcact gccgcactga tgcctgcatt gggcagcatt aagccgatgc gttcgccttc
    69301 aacactgtat ttttcaagga tgcgtccaac aaacagcgtt ttcgtaagca atttgcgata
    69361 ggagtctggg gtaaagttga cgtcttcgac acatttcttc ccggctccga agcggtacat
    69421 tgcactcagt aaagattcgt acagcgtttc acgcgggcgc accgccattc gcgcttccat
    69481 cattatttga tgcagcattt cgccagcgat tttgcgacgg tcacgggcac gcggcgcatc
    69541 cggcatctcc acctgcgttg gtggcaaaat atgcagagta atttgcggga acaagcgacg
    69601 tttaaccaga cctttcaggc ggctgaagtg cgtgagttcc gccccttcaa tacgcacagg
    69661 aataaccgtt gcaccagact tcgccgcgac aaaacccgcg ccatcgtaga ttttcatcag
    69721 cgagcctgta gtggtgatgc gtccttcagg gaaaatcacc actggtcggc cctgttccac
    69781 cagacgtacc agatgtttaa tagccatagg ttgcgtcggg tcgagaggaa caaagtcgat
    69841 aaatgatttc agccaacgca tataccactg ttggcttatt gaggtgtaaa cggcaaacac
    69901 tggacgcaca ggtaaaaaca gtccaagcaa aatgccatca ataaaagaga cgtgattagg
    69961 cgtaattaga acgcgctcgc ccttcagtgc ctgggtgtca cccgtaacgc gaacgcgata
    70021 caaaacacgg cacaaatttc gaaaaaagct aaaaagcatt ccaactccct ttgctctgat
    70081 tcagtaaaag cgaatggagg gagattacac gagataaaga acgcgagcga cagtaaatta
    70141 ggtgcgaaaa aaaacctgcg catccgcgca ggttggtgca agagacaggg tacgaagagc
    70201 gtaccgaata atctcaccaa tcaatacctc tgggatcttg attgtggtct gcacgacgac
    70261 tcttcgccag cgagaaaacg caaaggaatg aagggaaatg caacgaggtg tgtaaattgt
    70321 cggttactgt tacagattga tgaccggcca aaaaaaacct gcgcatctgc gcaggctggt
    70381 gtaattcatg tgctcaaccc gaagttgact tcacctatca atacctctgg gatcaccact
    70441 ttagcaacct gaagccaaac gccaccagcg gtcaatcgca acagcatttc gcaaagtgta
    70501 accaaaggtt tgcaatctct ttttttgtct tacttatttg cgtttggctc acattcccac
    70561 gatgaaaaca cgccacccct tgaaccaacg ggcgttttcc gtaacactga aagaatgtaa
    70621 gcgtttaccc actaaggtat tttcatggcg accataaagg atgtagcccg actggcaggc
    70681 gtttcagtcg ccaccgtttc ccgcgtcatt aataattcac ctaaagccag cgaagcttcc
    70741 cggctggctg tgcatagtgc aatggagtct cttagctatc acccgaacgc caacgcccgt
    70801 gcgctggcgc agcagaccac tgaaacggtc ggtctgatcg ttggtgatgt ttccgatccg
    70861 tttttcggtg caatggtgaa agcggtcgaa caggtggctt atcacaccgg taatttctta
    70921 ttgattggca acggttacca caacgaacaa aaagagcgtc aggctattga gcaactgatc
    70981 cgccatcgct gtgctgcgtt ggtcgtccat gccaaaatga tcccggatgc cgatttagcc
    71041 tcattaatga aacaaatgcc cggtatggtg ctgatcaacc gtatcctgcc tggctttgaa
    71101 aaccgttgta ttgctcttga cgatcgttac ggtgcctggc tggcaacgcg tcatttaatt
    71161 cagcaaggtc atacccgcat tggttatctg tgctctaacc actctatttc tgacgccgaa
    71221 gatcgtctgc aagggtatta cgatgccctt gctgaaagtg gtattgcggc caatgaccgg
    71281 ctggtgacat ttggcgaacc agacgaaagc ggcggcgaac aggcaatgac cgagcttttg
    71341 ggacgaggaa gaaatttcac tgcggtagcc tgttataacg attcaatggc ggcgggtgcg
    71401 atgggcgttc tcaatgataa tggtattgat gtaccgggtg agatttcgtt aattggcttt
    71461 gatgatgtgc tggtgtcacg ctatgtgcgc ccgcgcctga ccaccgtgcg ttacccaatc
    71521 gtgacgatgg cgacccaggc tgccgaactg gctttggcgc tggcggataa tcgccctctc
    71581 ccggaaatta ctaatgtctt tagtccgacg ctggtacgtc gccattcagt gtcaactccg
    71641 tcgctggagg caagtcatca tgcaaccagc gactaaccgc agttaaagca attccagcgc
    71701 cagtaattct tcgatggtct ggcgacggcg aatcaaccgc gcctgaccat tatcaaacag
    71761 aacttctggt aacagcggac ggctgttgta gttggatgac attgatgcgc catatgcccc
    71821 tgtatcatgc agtaccagat aatcacccgc cttcacttcc ggcaaggcgc gggtttcaac
    71881 atttccccct tcctgctggg taaagacatc gcccgattca cataatggcc cggcgacgac
    71941 ggtttccacc gttggcgcgt gttccagaga acgaccatca gctgccaggg cactgatatg
    72001 gtggtaacta ccgtacattg ccgggcgcat cagatcgttg aacccagcat caaccagcac
    72061 aaagtggcgg ctacccattt gtttgacgct ccgcacctga gtaattaata cgccagactg
    72121 cgctaccagg aagcgacccg gttcaatttc cagtttcaca gggtggccca aatggcgggc
    72181 gatttgctca cgcgcggcat tccacagacc ataataatgt tcggtatcaa ccgcctcttc
    72241 accctgttga taaggaacag aaagcccacc gcccgcagaa atagcctgta aatcctgacc
    72301 gaattcgatg acctgacgca ccatagcacc acacacctgt tccagatggg cataatcaac
    72361 gccagaacca atgtgcatgt gaatgccgac cagctgcaga tgatgacgtt gtatcacgtc
    72421 cagtgcggcg ggcagatcgg tgtaccagat accgtgcttg ctgttttcgc caccggtatt
    72481 ggttttttgg ctatgtccgt gaccaaaccc cggattaacg cgcagccata cccgatgccc
    72541 tggcgaaacc tggcccagtt ggtcgagcat atcaacagaa cccgcattca ccggaatttg
    72601 caattcactg acgcgttcaa gcgtcgcctg atcgataaca tctgccgtaa aaacaatatc
    72661 atcggggtgc gtttgcggat tgtaacccgc cgccaacgca cgctctattt cgcctaacga
    72721 gacggaatcc actttcacgc cctgctcacg cattaagcgc aaaatatgaa tattggaaca
    72781 ggctttctgt gcaaagcgca ccacatcaaa ctgtttcagc gctgcaatct gccgacgaat
    72841 aatttgcgca tcgtagaccc acaccgggca gccaaattca gcgggcaaac gcagcagatt
    72901 ttcggcggtg agatcggtat cggtgctgaa cagtgaatgt ggcataacaa actccagata
    72961 agtgcttttt tatgattacg ccacatcata aaaagaataa aaaatatcga tttatgtcga
    73021 gtctatgcaa aaatgatatg gattaccgga ttgcgagaga gcgctaatgg ccgccgttaa
    73081 cttacgtcat attgaaattt ttcatgcggt aatgaccgcc ggaagcctga ctgaggcagc
    73141 acacctgcta cacacctcac agccaaccgt cagccgcgaa ctggcgcgct ttgagaaggt
    73201 gatcgggcta aaattgtttg agcgcgtacg tggacgatta catcctaccg tgcaaggact
    73261 gcgtctgttt gaagaagtgc aacgatcctg gtacggactg gatcgcattg tcagcgccgc
    73321 agaaagtctg cgcgagtttc gccagggaga actgtctatt gcctgcctgc cggtcttttc
    73381 gcaatctttt ttaccgcagc tcctgcaacc ctttctggca cgttatcccg atgtcagctt
    73441 aaatatcgtg ccccaggaat caccgctact tgaagagtgg ctctcggccc agcgtcatga
    73501 tttaggactc actgaaacgc tccatacgcc tgcgggaaca gaacgtaccg aattactctc
    73561 tttagatgaa gtgtgtgtgt tacctccggg tcatccgctg gcggtaaaaa aggtattaac
    73621 gccggatgat tttcagggtg agaactacat cagcctttcc cgtactgaca gctatcgcca
    73681 gttgctggat cagctattta ctgaacatca ggttaaacga cgcatgatcg tagaaaccca
    73741 cagcgccgcg tcagtctgcg caatggtacg ggcgggggta ggtgtttcgg tggttaaccc
    73801 gctcaccgca ctggattatg cggcaagtgg tttagtggtg cggcggttca gtattgcggt
    73861 tccgttcacc gtcagcctga tccgccccct gcaccgcccg tcatcagcgc tggtgcaggc
    73921 gtttagtgag catttacaag cggggttacc gaaactggtc acttctcttg acgctatttt
    73981 gtcgtcagct acgacagcat aaaagcgaca gcatcctcgg catggatcgc cgcagtatca
    74041 aacacaggca aaacactgcg ctcttctggc accagtaaac caatttctgt gcagccaaaa
    74101 atgacgccct gtgcgccctg ttctgcaagg cgagcaatca cttgcgcata ataagcgcgt
    74161 gacgcttcgg taaattgccc cagacacagt tcttcaaaaa taatctgatt aattttcgcc
    74221 cgttcatccg cttcaggaat aagacaattg atggaaaatt gttccatcag ccgcccgcga
    74281 taaaaatcct gttccatggt gtaacgcgta cccagcagcg ccacacgagt cattccggcc
    74341 ccggtaattg cacgtccggt ggcatccgca atgtgtaaga aaggcagagt gcaacgtgac
    74401 tcaatggcat ccgccacttt atgcatcgta ttggtacata acacaatacc ttctgcgccc
    74461 gcccgctgta agccaagcgc cgcctcagcc agaatgtccc cggttttatc ccattccccg
    74521 cgacgctggc actcttctat ttcatgaaaa tcgacgctat gtagcagcac ttgcgcagag
    74581 tgaagcccac caagccgctg tttaatgcct tcatttatca gacgatagta aggaatggtg
    74641 gattcccagc tcattcctcc cagcaaacca attgttttca ttcctctctc ctgatgtgtg
    74701 ttaccgcaac aaggctgaat aaggaacgag acaaacgcct caacggccag gtgcccgatc
    74761 tctattaacg aaaaaagggc cggatgtaca gcacatccgg cccgtgaaat cagacgccga
    74821 tatttctcaa cttctcgcct gccatcagtt tgcgttcgat atgttccagc gtgacatttt
    74881 tggtttccgg aatgagccag aaagtgatgc ccacaaacgc aatgttcagc gcagtgtaga
    74941 gccagaacgt accggcagcg ccaatgctat caagcagtgt caggaaggtc gcgccgataa
    75001 tcatattcga cacccagttt gtcgtcgtcg aacaggtaat accgaaatcg cggcatttca
    75061 gcggctgaat ttcagagcac aggatccaca ccactggcgc ggcgctcatc gcataaccgg
    75121 caatacacat catcgtcatg ccaacagaga gccaggacaa gccactggaa gccgtaccgt
    75181 tatcaaactg catcaggcaa tagcccagca ccagagtgcc taacgccatc acgctgaaac
    75241 caattttcag agccggttta cgacctgctt tatctaccgt aaagaccgca atgaaggtcg
    75301 cgaacataaa ggtcagtccc acgaccagag tcgcaatcat ctgttgttct gtggtcgtaa
    75361 agcccgccat tttgaagata cgcggcgcat agtacatgat gatgttcata ccggtaaact
    75421 gctgcatcgc ctgcaacaac ataccgagaa acacagcacg acggacgtta cggttgatct
    75481 taaacagtgc ccaaccgcct tgttttaact tcaggctttc acgaatttcg ttgagttctt
    75541 ctcgcgcttt ttccgacgta tcgcgcagca tacgcaacac ttcttccgcc tcaatatgac
    75601 gccccttttc cgccagccag cgcgggctat ttggcaggaa gaccaccaga ataatcagca
    75661 ggactgctgg taaagcaaga acccccaaca ttgcgcgcca gttaccgcta taactgaacg
    75721 ctgtatcgga taaaaacgcc agcacgatgc cgagtgtgac catcaactgg tacatactga
    75781 tcatcttacc gcgaacgttt tcacttgcca tttcagaaag atacagagga gcggtgtaag
    75841 acgcgatccc gacagcaatg cccagcacca cccgagcggc gattaacatc tctacgctgg
    75901 tcgcaaaagc ggaccctata gaaccgagta caaacaggat ggcccccgcc atcaggctgt
    75961 atttacgacc caggcggaac gacagccaac cattaaacag cgcaccaatt gctgcgccga
    76021 gcatcatgct actaaccacc cattcctgca aacgactggt cagcacaaag tgatcggtaa
    76081 tgaacggcaa cgctccggcg attacgccga tatcaagacc aaataacaat cctgcgaccg
    76141 cagcagctac cgaaacaaac atattcatac gccgcgtatc ccgcaaagaa cgtggcgtta
    76201 aagcagattc cgtattgata gtaaccattt tttcctgcca gcagagagta agacatagtg
    76261 aaaaaatacg tgaacaactc acgcaggtgt caggtcggaa acagcataaa tatggattaa
    76321 attgctacga catgtcgtta tgtgatggat attccaattt
//
LOCUS       DAPHDW010000003        73363 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.003, whole genome shotgun sequence.
ACCESSION   DAPHDW010000003 DAPHDW010000000
VERSION     DAPHDW010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 73363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.003"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..378
                     /locus_tag="RH057_000148"
     CDS             <1..378
                     /locus_tag="RH057_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2170 family protein"
                     /protein_id="HDV3126572.1"
                     /translation="ATALQTVPEQNIDVTNSESALIIKMNDYGDLQINILFTSRQMII
                     ETFICPVSSISNPDEFNTFLLRNQKMMPLSSVGISSVQQEEYYIVFGALSLKSSLEDI
                     LLEITSLVDNALDLAEITEEYSH"
     gene            397..1095
                     /locus_tag="RH057_000149"
     CDS             397..1095
                     /locus_tag="RH057_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspA/IM30 family protein"
                     /protein_id="HDV3126573.1"
                     /translation="MGILKSLFTLGKSFISQAEESIEETQGVRMLEQHIRDAKAELDK
                     AGKSRVDLLARVKLSHDKLKDLRERKASLEARALEALSKNVNPSLINEVAEEIARLEN
                     LITAEEQVLSNLEVSRDGVEKAVTATAQRIAQFEQQMEVVKATEAMQRAQQAVTTSTV
                     GASSSVSTAAESLKRLQTRQAERQARLDAAAQLEKVADGRDLDEKLAEAGIGGSNKSS
                     AQDVLARLQRQQGE"
     gene            1146..1805
                     /locus_tag="RH057_000150"
     CDS             1146..1805
                     /locus_tag="RH057_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjfK family protein"
                     /protein_id="HDV3126574.1"
                     /translation="MSGFFQRLFGKDNKPAIARGPLGLHLNSGFTLDTLAFRLLEDEL
                     LIALPGEEFTVAAVSHIDLGGGSQIFRYYTSGDEFLQINTTGGEDIDDIDDIKLFVYE
                     ESYGISKESHWREAINAKVMGAMILNWQEKRWQRFFNSEEPGNIEPVYMLEKVENQNH
                     AKWEVHNFTMGYQRQVTEDTYEYLLLNGEESFNDLGEPEWLFSRALGVDIPLTSLHII
                     G"
     gene            1823..2221
                     /locus_tag="RH057_000151"
     CDS             1823..2221
                     /locus_tag="RH057_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HDV3126575.1"
                     /translation="MHILDSLLAFSAYFFIGVAMVIIFLFIYSKITPHNEWQLIKNNN
                     TAASLAFSGTLLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMPALSE
                     KIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            2231..2869
                     /locus_tag="RH057_000152"
     CDS             2231..2869
                     /locus_tag="RH057_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HDV3126576.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPHNPFEPRRNRYAQKYLTLVL
                     MGGAVFLIVKGWSESSDVDNDGDGTFYSTVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVISGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRSTSGDYSWRSGSGKKESYSSGGFTTKKASTVSRGGYGRSSSARGHWGG"
     gene            2872..4035
                     /locus_tag="RH057_000153"
     CDS             2872..4035
                     /locus_tag="RH057_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV3126577.1"
                     /translation="MLRHNVPVRRDLDQIAADNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSREPFYFCCCQDTDEDRTTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLCKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            4119..5744
                     /locus_tag="RH057_000154"
     CDS             4119..5744
                     /locus_tag="RH057_000154"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HDV3126578.1"
                     /translation="MHWQTHTVFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPITMTFAATPLLLQMLPAPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTTGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVICKRGMPF
                     VAEAMEVLGGIGYCEESELPRLYREMPVNSIWEGSGNIMCLDVLRVLNKQAGVYDLLS
                     EAFVEVKGQDRYFDRAVRRLQQQLRKPAEELRREITHQLFLLGCGAQMLKYASPPMAQ
                     AWCQVMLDTRGGVRLSEQIQNDLLLRATGGVCV"
     gene            complement(5861..6136)
                     /gene="yjfN"
                     /locus_tag="RH057_000155"
     CDS             complement(5861..6136)
                     /gene="yjfN"
                     /locus_tag="RH057_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HDV3126579.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(6285..6614)
                     /gene="bsmA"
                     /locus_tag="RH057_000156"
     CDS             complement(6285..6614)
                     /gene="bsmA"
                     /locus_tag="RH057_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HDV3126580.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            6796..7545
                     /gene="yjfP"
                     /locus_tag="RH057_000157"
     CDS             6796..7545
                     /gene="yjfP"
                     /locus_tag="RH057_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV3126581.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDAARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNNIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(7542..8297)
                     /gene="ulaR"
                     /locus_tag="RH057_000158"
     CDS             complement(7542..8297)
                     /gene="ulaR"
                     /locus_tag="RH057_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HDV3126582.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLD
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(8405..9469)
                     /gene="ulaG"
                     /locus_tag="RH057_000159"
     CDS             complement(8405..9469)
                     /gene="ulaG"
                     /locus_tag="RH057_000159"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HDV3126583.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEMKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            9824..11221
                     /gene="ulaA"
                     /locus_tag="RH057_000160"
     CDS             9824..11221
                     /gene="ulaA"
                     /locus_tag="RH057_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV3126584.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASWIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVNWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWAVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            11237..11542
                     /gene="ulaB"
                     /locus_tag="RH057_000161"
     CDS             11237..11542
                     /gene="ulaB"
                     /locus_tag="RH057_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HDV3126585.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            11552..12016
                     /gene="ulaC"
                     /locus_tag="RH057_000162"
     CDS             11552..12016
                     /gene="ulaC"
                     /locus_tag="RH057_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HDV3126586.1"
                     /translation="MKLRDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            12030..12680
                     /gene="ulaD"
                     /locus_tag="RH057_000163"
     CDS             12030..12680
                     /gene="ulaD"
                     /locus_tag="RH057_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV3126587.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            12690..13544
                     /gene="ulaE"
                     /locus_tag="RH057_000164"
     CDS             12690..13544
                     /gene="ulaE"
                     /locus_tag="RH057_000164"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HDV3126588.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLQLAKTLGFDFVEMSVDETD
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVAKARDWVKARMA
                     KAGMVEAA"
     gene            13544..14230
                     /gene="ulaF"
                     /locus_tag="RH057_000165"
     CDS             13544..14230
                     /gene="ulaF"
                     /locus_tag="RH057_000165"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HDV3126589.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSFLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(14359..14634)
                     /locus_tag="RH057_000166"
     CDS             complement(14359..14634)
                     /locus_tag="RH057_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3126590.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            14961..15356
                     /gene="rpsF"
                     /locus_tag="RH057_000167"
     CDS             14961..15356
                     /gene="rpsF"
                     /locus_tag="RH057_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HDV3126591.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            15363..15677
                     /gene="priB"
                     /locus_tag="RH057_000168"
     CDS             15363..15677
                     /gene="priB"
                     /locus_tag="RH057_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HDV3126592.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            15682..15909
                     /gene="rpsR"
                     /locus_tag="RH057_000169"
     CDS             15682..15909
                     /gene="rpsR"
                     /locus_tag="RH057_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HDV3126593.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            15951..16400
                     /gene="rplI"
                     /locus_tag="RH057_000170"
     CDS             15951..16400
                     /gene="rplI"
                     /locus_tag="RH057_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HDV3126594.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(16512..17483)
                     /locus_tag="RH057_000171"
     CDS             complement(16512..17483)
                     /locus_tag="RH057_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001422748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HDV3126595.1"
                     /translation="MKIKVSAGIIGAVLMLSASQSWAVTLKLSHNQDKSHPVHKAMEF
                     FAKKSKEYSNGDITIRIYPNGTLGTQRETMELIRSGAIPLVKTNAAEMEAFENSYKLF
                     SLPYLFRDRDHYYQVMQGDIGRKILDSTKSKGYFGLTFYDGGARSFYGNKPVLKPDDL
                     KGMKVRVQPSPGAVEMIKVMGGNPTPLDYGELYTALQQGVVDMAENSVMALTTMRHGE
                     VAKSFSLDEHTMVPDVVLMSNAAFDKLSPENQAVILKAAKESMSYMKDLWSEEEKQEF
                     AKLDKMGVKVYQVDKAPFIEKVQPMYANFAKDNPALAPMLADIQAAK"
     gene            complement(17536..18837)
                     /locus_tag="RH057_000172"
     CDS             complement(17536..18837)
                     /locus_tag="RH057_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HDV3126596.1"
                     /translation="MDYWLPIIVLFGAFFFMLALGVPIVYAIGLSTLASISTQLDFNS
                     ALSVVSQKLASGLDSFTLLAIPFFILSGNIMNHGGIARRLINFARILGGRLPGSLAHC
                     NILANMLFGAISGSAVASAAAMGGVMHPQQVKEGYDPAFSTAVNVASAPTGLLIPPSN
                     TLIVYSLVSGGTSIAALFLAGYVPGILLGLALMVIAGIIAVRRGYPKPERPTLRQAGV
                     AIWMAIPSIFLIILIMGGVLSGIFTPTEASAIAVIYTLFLALVLYREISVKDLPKIFL
                     ESVITTAIVLLLIGSSMGMSWAMSNADVPFLILDLLNTISDNPIIILLIINIILLIIG
                     TFMDMTPAVLIFTPIFLPVVTELGMDPIHFGIVMVLNMCIGICTPPVGSVLFVGCSVS
                     KLPINKIIKPMLPFYAVMVLVLAMVTYIPQISMALPRALGY"
     gene            complement(18880..19356)
                     /locus_tag="RH057_000173"
     CDS             complement(18880..19356)
                     /locus_tag="RH057_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012311688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HDV3126597.1"
                     /translation="MRNIIRSINALLAALNITILAIIVACVTWQVAARFIFTSPSIFT
                     DELSRLLFICLGLFGGAYTAGQNRHLAIDLLPMMLKGKARRHLFLCIQIIVIIFATII
                     MVYGGGLLTMDTFDSGQTSPALGWQMGYIYMSIPISGVLIIIYTIDMVLTELKQPL"
     gene            complement(19377..21458)
                     /locus_tag="RH057_000174"
     CDS             complement(19377..21458)
                     /locus_tag="RH057_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005766390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HDV3126598.1"
                     /translation="MSQLDDTILDALTHVTFPKGFAQAEPAWVVTVDGVDYPLWQTDA
                     LVVGSGAAGLRAAVELKRRQQNVLIATAGLYMGTSACSGSDKQTLFTAATAGNGDNFT
                     KLAEALASGGAMDHDTAYVEAVGSLHTLGGLQYLGLELPEDRYGAILRYQTDHDEAGR
                     ATSCGPRTSRLMVKVLLEEVQRLAIPVLTSATVIKLLHQRDENGEDRVAGAILATGHR
                     AHNPWGLAIVTAPNVVLATGGPGELYRDSVYPHKCFGSLGLALEEGLTLTNLTESQFG
                     IGTPRSTFPWNLSGTYVQVIPYIYSVDAEGNEYNFLADYYRTTQELASNIFRKGYQWP
                     FHATRVMDFGSSLLDMAVAQEQQSGRQVFMDFNRNPEAVPGDLPFSLDRLDDDVRAYL
                     ENNDALAPSPIERLQRMNPLSISLYKMHGYDLTTQPLQFAMNNQHMNGGIEVDIWGQT
                     SLPGCFAVGEVAGTHGVTRPGGAALNAGQVFAVRLARFIGCTQKRNINGDIAQLVAPT
                     LASIREIITQAHDNGTGMPLSVVREKIQARMSDHAGFICHADKVRRATRDALLLSEFV
                     QRHGLAIKHVGEVAELFMWRHMALTSAAVLTQLTHYIDAGGGSRGARIILDRDGNSIP
                     QTRNGFCDAWRFRSERTEDKKDKLLIHYCNGIFHVRETPVREFPIIRGIWFEKNWPGF
                     LNGTIYQPQDE"
     gene            complement(21455..22996)
                     /locus_tag="RH057_000175"
     CDS             complement(21455..22996)
                     /locus_tag="RH057_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV3126599.1"
                     /translation="MRKITTAEALAAQIQDGATIAISGNGGGMVEADHILAAIEARFL
                     QTGHPRYLTLIHSLGIGDRDCKGTNRFAHAEMLKRIIAGHFTWSPKMQALVKNNTIEA
                     YCFPGGVIQALLREIGAGRPGLFTHVGLGSFVDPRNGGGKSNECTTDELVELIEIDGE
                     TKLRYRPFKVDYAILRGTYADPRGNVSLEEEAIDMDSYSMALAAHNSGGKVFVQVRDV
                     LEAGAIEPRRVKLPGILVDGIVEHREQPQTYLGGYDLTISGQHRRLSSNDAIELVSHP
                     VRRLIARRAARELVAGASTNFGFGIPGGIPGVALREGVPYQSLWLSVEQGVHNGMMLD
                     DAFFGCARNADAIIPSLDQFEFYSGGGIDITFLGMGEMDQYGNVNVSHLNGNLIGPGG
                     FLEIAQNARKVVFCGTFDAKGSKIDVTPDGLHITQSGQIPKLVTQVEKITFSAAYAQQ
                     SGQEVLYITERAVFQLTAEGVELIEIAPGVEIERDILPYMAFRPIIKHPRLMESSLFT
                     PMEDA"
     gene            complement(23006..23782)
                     /locus_tag="RH057_000176"
     CDS             complement(23006..23782)
                     /locus_tag="RH057_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HDV3126600.1"
                     /translation="MSDQPVLFSRVAASCRLTLNREDKCHAINEEMIESLDHYLNEIE
                     NDTTLRLVELTATGDKFFCAGGDIKSWSAYSPLDMGRKWIKRGNDVFNRLRNIPQLTV
                     ANLNGHTIGGGIELALCCDIRIARPGAKFSNPEVMLGMVPGWMGIERVLNQVGPVVGR
                     QMLMLGKRLTAQEAQAANLIDEVVEKEQVESWMANQLAQLEKCGPVALAHIKQLILAL
                     ENKHADYPHQLLAGLMSATQDCQQATRAFAEKSSVSFHNQ"
     gene            complement(23792..24634)
                     /locus_tag="RH057_000177"
     CDS             complement(23792..24634)
                     /locus_tag="RH057_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV3126601.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQIAHQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDLASQILRAGKRILAFHVSDWLVPT
                     TDLVNDRGMPGDGVINIPSIRRLVENAGFNGAIELEIFSPYWWQKDINSTLDISVDRI
                     AHYC"
     gene            complement(24644..25435)
                     /locus_tag="RH057_000178"
     CDS             complement(24644..25435)
                     /locus_tag="RH057_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HDV3126602.1"
                     /translation="MKSTRPVAVITGAARGIGKGCALELARGGFNLLINDLPDADSVE
                     KLHITQQECIAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            25648..26295
                     /locus_tag="RH057_000179"
     CDS             25648..26295
                     /locus_tag="RH057_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HDV3126603.1"
                     /translation="MYLDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKHNIFKPDIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            complement(26279..26917)
                     /gene="ytfB"
                     /locus_tag="RH057_000180"
     CDS             complement(26279..26917)
                     /gene="ytfB"
                     /locus_tag="RH057_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YtfB"
                     /protein_id="HDV3126604.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDMPNAPVVTREAQLDIQSQSQPPTEEQLRAQLVTPQNDPDQVAPVAPEPIQ
                     EGQPEEQPQTTQTQPFQPDSGIDNQWRSYRVEPGKTMAQLFRDHGLPATDVYAMAQVE
                     GAGKPLSNLQNGQMVKIRQNASGVVTGLTIDTGNNQQVLFTRQPDGSFIRAR"
     gene            27136..27756
                     /gene="fklB"
                     /locus_tag="RH057_000181"
     CDS             27136..27756
                     /gene="fklB"
                     /locus_tag="RH057_000181"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV3126605.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            28065..29477
                     /gene="cycA"
                     /locus_tag="RH057_000182"
                     /gene_synonym="dagA"
     CDS             28065..29477
                     /gene="cycA"
                     /locus_tag="RH057_000182"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HDV3126606.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHIQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(29522..30184)
                     /gene="ytfE"
                     /locus_tag="RH057_000183"
     CDS             complement(29522..30184)
                     /gene="ytfE"
                     /locus_tag="RH057_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HDV3126607.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(30292..31257)
                     /locus_tag="RH057_000184"
     CDS             complement(30292..31257)
                     /locus_tag="RH057_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3126608.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            complement(31365..32225)
                     /gene="qorB"
                     /locus_tag="RH057_000185"
     CDS             complement(31365..32225)
                     /gene="qorB"
                     /locus_tag="RH057_000185"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV3126609.1"
                     /translation="MIAITGATGQLGHYVIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYGDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYTENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYAAAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKSVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGRPTTTLAE
                     SVSHLFNVNN"
     gene            32314..32694
                     /locus_tag="RH057_000186"
     CDS             32314..32694
                     /locus_tag="RH057_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV3126610.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            complement(32823..34766)
                     /gene="cpdB"
                     /locus_tag="RH057_000187"
     CDS             complement(32823..34766)
                     /gene="cpdB"
                     /locus_tag="RH057_000187"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HDV3126611.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDIHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDAKTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     LVVVLAHSGLSADPYKVMAENSVYYLSEIPGVDAIMFGHAHAVFPSKDFADIEGADIA
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     SDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIDPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAGDKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            34956..35696
                     /gene="cysQ"
                     /locus_tag="RH057_000188"
     CDS             34956..35696
                     /gene="cysQ"
                     /locus_tag="RH057_000188"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HDV3126612.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLTPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKQIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            complement(35686..36243)
                     /locus_tag="RH057_000189"
     CDS             complement(35686..36243)
                     /locus_tag="RH057_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV3126613.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFETGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEKSSAVVVLDKDGRVQ
                     WAKDGALTQEEVQQVMDLLHKLINK"
     gene            36568..36774
                     /locus_tag="RH057_000190"
     CDS             36568..36774
                     /locus_tag="RH057_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HDV3126614.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDVGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            complement(36836..38179)
                     /locus_tag="RH057_000191"
     CDS             complement(36836..38179)
                     /locus_tag="RH057_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV3126615.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANI
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(38502..39140)
                     /gene="msrA"
                     /locus_tag="RH057_000192"
     CDS             complement(38502..39140)
                     /gene="msrA"
                     /locus_tag="RH057_000192"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HDV3126616.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            39346..41079
                     /gene="tamA"
                     /locus_tag="RH057_000193"
     CDS             39346..41079
                     /gene="tamA"
                     /locus_tag="RH057_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HDV3126617.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQNVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFAGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            41076..44855
                     /gene="tamB"
                     /locus_tag="RH057_000194"
     CDS             41076..44855
                     /gene="tamB"
                     /locus_tag="RH057_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HDV3126618.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQPGVAVKAGNLHLAVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVAEVAQEEVVEPKIENPQPDEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVNTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGILSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPQTTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTQGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            44858..45199
                     /locus_tag="RH057_000195"
     CDS             44858..45199
                     /locus_tag="RH057_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HDV3126619.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            45411..45662
                     /gene="chpS"
                     /locus_tag="RH057_000196"
     CDS             45411..45662
                     /gene="chpS"
                     /locus_tag="RH057_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin ChpS"
                     /protein_id="HDV3126620.1"
                     /translation="MRITIKRWGNSAGMVIPNIVMKELNLQPGQSVEAQVSNNQLILT
                     PISRRYSLDELLAQCDMNAAELSEQDVWGKSTPAGDEIW"
     gene            45656..46006
                     /gene="chpB"
                     /locus_tag="RH057_000197"
     CDS             45656..46006
                     /gene="chpB"
                     /locus_tag="RH057_000197"
                     /EC_number="4.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease toxin ChpB"
                     /protein_id="HDV3126621.1"
                     /translation="MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLG
                     MTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLVNQVRMMDLRARLAKRIGLAADE
                     VVEEALLRLQAVVE"
     gene            complement(46086..46616)
                     /gene="ppa"
                     /locus_tag="RH057_000198"
     CDS             complement(46086..46616)
                     /gene="ppa"
                     /locus_tag="RH057_000198"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HDV3126622.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVGVLKMTDEAGE
                     DAKLIAVPHTKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            46926..47882
                     /gene="ytfQ"
                     /locus_tag="RH057_000199"
     CDS             46926..47882
                     /gene="ytfQ"
                     /locus_tag="RH057_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
                     /protein_id="HDV3126623.1"
                     /translation="MWKRLLVVSAVSAAMSSMALAAPLTVGFSQVGSESGWRAAETNV
                     AKSEAEKRGITLKIADGQQKQENQIKAVRSFVAQGVDAIFIAPVVATGWEPVLKEAKD
                     AEIPVFLLDRSIDVKDKSLYMTTVTADNILEGKLIGDWLVKEVNGKPCNVVELQGTVG
                     ASVAIDRKKGFAEAIKNAPNIKIIRSQSGDFTRSKGKEVMESFIKAENNGKNICMVYA
                     HNDDMVIGAIQAIKEAGLKPGKDILTGSIDGVPDIYKAMIDGEANASVELTPNMAGPA
                     FDALEKYKKDGTMPEKLTLTKSTLYLPDTAKEELEKKKNMGY"
     gene            48192..49694
                     /locus_tag="RH057_000200"
     CDS             48192..49694
                     /locus_tag="RH057_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3126624.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQAISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGFLRRKEMEKRATELMTSYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEVELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKQQNLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDEPTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAELSVPAIMNAIAA"
     gene            49708..50730
                     /locus_tag="RH057_000201"
     CDS             49708..50730
                     /locus_tag="RH057_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3126625.1"
                     /translation="MPQSLPDTTPPKRRFRWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGMTLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEAVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            50717..51712
                     /gene="yjfF"
                     /locus_tag="RH057_000202"
     CDS             50717..51712
                     /gene="yjfF"
                     /locus_tag="RH057_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
                     /protein_id="HDV3126626.1"
                     /translation="MIKRNLPLMITIGVFVLGYLYCLTQFPGFASTRVICNILTDNAF
                     LGIIAVGMTFVILSGGIDLSVGSVIAFTGVFLAKVIGDFGLSPLLAFPLVLVMGCAFG
                     AFMGLLIDALKIPAFIITLAGMFFLRGVSYLVSEESIPINHPIYDTLSSLAWKIPGGG
                     RLSAMGLLMLAVVVIGIFLAHRTRFGNQVYAIGGNATSANLMGISTRSTTIRIYMLST
                     GLATLAGIVFSIYTQAGYALAGVGVELDAIASVVIGGTLLSGGVGTVLGTLFGVAIQG
                     LIQTYINFDGTLSSWWTKIAIGILLFIFIALQRGLTVLWENRQSSPVTRVNIAQR"
     gene            complement(51745..52743)
                     /gene="fbp"
                     /locus_tag="RH057_000203"
     CDS             complement(51745..52743)
                     /gene="fbp"
                     /locus_tag="RH057_000203"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HDV3126627.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            52919..54292
                     /gene="mpl"
                     /locus_tag="RH057_000204"
     CDS             52919..54292
                     /gene="mpl"
                     /locus_tag="RH057_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HDV3126628.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLDPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(54443..54994)
                     /locus_tag="RH057_000205"
     CDS             complement(54443..54994)
                     /locus_tag="RH057_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HDV3126629.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRAAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            55088..56440
                     /gene="pmbA"
                     /locus_tag="RH057_000206"
     CDS             55088..56440
                     /gene="pmbA"
                     /locus_tag="RH057_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HDV3126630.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPDLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            56623..57009
                     /gene="cybC"
                     /locus_tag="RH057_000207"
     CDS             56623..57009
                     /gene="cybC"
                     /locus_tag="RH057_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
                     /protein_id="HDV3126631.1"
                     /translation="MRKSLLAILAVSSLVFSSASFAADLEDNMETLNDNLKVIEKADN
                     AAQVKDALTMMRAAALDAQKATPPKLEDKSPDSPEMKDFRHGFDILVGQIDDALKLAN
                     EGKVKEAQAAAEQLKTTRNAYHQKYR"
     gene            complement(57054..57518)
                     /gene="nrdG"
                     /locus_tag="RH057_000208"
     CDS             complement(57054..57518)
                     /gene="nrdG"
                     /locus_tag="RH057_000208"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HDV3126632.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDQIINDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVQRIRAECPGKDIW
                     VWTGYKLDELNATQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(57676..59814)
                     /gene="nrdD"
                     /locus_tag="RH057_000209"
     CDS             complement(57676..59814)
                     /gene="nrdD"
                     /locus_tag="RH057_000209"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HDV3126633.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVSEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNSSLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVKAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVKVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFGGEHVY
                     DNEQLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(60208..61863)
                     /gene="treC"
                     /locus_tag="RH057_000210"
     CDS             complement(60208..61863)
                     /gene="treC"
                     /locus_tag="RH057_000210"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HDV3126634.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQKINVEAALA
                     DESSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(61913..63334)
                     /gene="treB"
                     /locus_tag="RH057_000211"
     CDS             complement(61913..63334)
                     /gene="treB"
                     /locus_tag="RH057_000211"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HDV3126635.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(63453..64400)
                     /gene="treR"
                     /locus_tag="RH057_000212"
     CDS             complement(63453..64400)
                     /gene="treR"
                     /locus_tag="RH057_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HDV3126636.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMESQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            64585..64638
                     /gene="mgtL"
                     /locus_tag="RH057_000213"
     CDS             64585..64638
                     /gene="mgtL"
                     /locus_tag="RH057_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HDV3126637.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            64779..67475
                     /gene="mgtA"
                     /locus_tag="RH057_000214"
     CDS             64779..67475
                     /gene="mgtA"
                     /locus_tag="RH057_000214"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HDV3126638.1"
                     /translation="MFKEIFTRLIRHLPSRLVHRDPLPGAQQTVNTVVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGENKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDIMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKMTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMIFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(67681..68067)
                     /gene="ridA"
                     /locus_tag="RH057_000215"
     CDS             complement(67681..68067)
                     /gene="ridA"
                     /locus_tag="RH057_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HDV3126639.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(68140..68601)
                     /gene="pyrI"
                     /locus_tag="RH057_000216"
     CDS             complement(68140..68601)
                     /gene="pyrI"
                     /locus_tag="RH057_000216"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HDV3126640.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEDQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(68614..69549)
                     /gene="pyrB"
                     /locus_tag="RH057_000217"
     CDS             complement(68614..69549)
                     /gene="pyrB"
                     /locus_tag="RH057_000217"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HDV3126641.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(69553..69687)
                     /gene="pyrL"
                     /locus_tag="RH057_000218"
     CDS             complement(69553..69687)
                     /gene="pyrL"
                     /locus_tag="RH057_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HDV3126642.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCPGVRR
                     "
     gene            complement(69968..70363)
                     /locus_tag="RH057_000219"
     CDS             complement(69968..70363)
                     /locus_tag="RH057_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV3126643.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQFEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEQ"
     gene            complement(70494..71207)
                     /gene="bdcA"
                     /locus_tag="RH057_000220"
     CDS             complement(70494..71207)
                     /gene="bdcA"
                     /locus_tag="RH057_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3126644.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDANPANGPMRDMLHGFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            71278..71871
                     /locus_tag="RH057_000221"
     CDS             71278..71871
                     /locus_tag="RH057_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HDV3126645.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVAEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLVDILRDDRPVGECLAEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAVQYYHAAETAIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSLEQLCATAALAGGAIKTILKE"
     gene            72016..72468
                     /gene="tabA"
                     /locus_tag="RH057_000222"
     CDS             72016..72468
                     /gene="tabA"
                     /locus_tag="RH057_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HDV3126646.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTVETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGTPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            72591..>73363
                     /locus_tag="RH057_000223"
     CDS             72591..>73363
                     /locus_tag="RH057_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV3126647.1"
                     /translation="MSKISGWNFSQNITSADNCKQKNEDLDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKMFTLGYVDFSKRSNEAGRNMMAHIKSSSYIKNNDGSEIMKF
                     VMNNPEGERADLSKVEIEITLASAFNNGIREGHTVIIFPQPDGSTNRYEGKSFERKDE
                     SSLHLITNKILACYQREANKEIARLLNISQELNNSQDLNNSQVSCKDSVDSTITDLLE
                     KPLNNALLAIRKEHLLLMPYVCNESISYLLGEKGILKEI"
BASE COUNT        17678 a        18225 c        19376 g        18084 t
ORIGIN      
        1 gcgacggcgc tgcaaactgt acctgaacaa aatattgatg tgacaaatag cgagagcgca
       61 ttaattatta aaatgaatga ttatggcgat ttgcaaatca atattctttt tacttcccgc
      121 caaatgatta tcgaaacctt tatttgtccg gtaagtagta tcagcaatcc agatgaattt
      181 aataccttct tattaagaaa tcagaagatg atgccgttgt catcggtagg aatctccagc
      241 gtgcaacagg aagagtatta cattgttttc ggggcgttat cgcttaaatc ttctcttgaa
      301 gatatcctgc ttgagataac ctcgctggta gataacgcat tggatctggc tgaaattaca
      361 gaagaatatt cacactaatt acaaggacgg gtatttatgg gaattttaaa aagtttattt
      421 acgctgggga aatcgtttat ctcccaggcg gaagaatcca ttgaagaaac ccagggcgtg
      481 cgcatgctgg aacagcatat tcgtgacgct aaagctgaac tcgataaagc cggaaaatct
      541 cgcgttgatc tgctggcgcg ggtgaaatta agtcacgata agctgaaaga tttacgtgag
      601 cgcaaagcca gtctggaagc ccgtgcgctg gaagcgttaa gcaagaacgt taatccgtcg
      661 ttgattaacg aagttgctga agaaatcgcg cgccttgaga atctcattac cgctgaagag
      721 caagtgttgt cgaatctgga agtctcccgt gatggcgtgg aaaaagcagt tacagcgaca
      781 gcgcagcgta tcgctcagtt tgagcagcaa atggaagtcg ttaaagccac tgaagccatg
      841 cagcgtgcac aacaggctgt aacaacctct accgttggcg catcttccag cgtttcgaca
      901 gcggcagaat ccttaaaacg cctgcaaacg cgtcaggccg aacgtcaggc tcgcctggat
      961 gctgctgcac agttggaaaa agtcgcagac ggtcgcgatc ttgatgaaaa gctggcggaa
     1021 gccggaattg gcggtagcaa taaaagtagc gcccaggatg tattagcaag actgcaacgc
     1081 caacagggcg agtaattttt ttgccagcct cgctaaaagg ctggcaacta ttttaaggat
     1141 aaaatatgtc tggttttttc cagcgtctgt ttggcaagga taataagcct gctatcgctc
     1201 gcggtccgct gggacttcat ctcaatagtg gctttacgct cgacacgtta gcgtttcgtt
     1261 tgctggaaga tgaattgctg attgcgctgc cgggtgagga atttacggta gccgctgtca
     1321 gccacatcga tctgggcggc ggtagtcaga ttttccgcta ctacacttca ggcgatgaat
     1381 ttctgcaaat caataccacc ggcggcgaag atattgatga cattgatgat atcaagctct
     1441 ttgtctatga agagagctac ggtatcagta aagagagtca ctggcgcgag gcgatcaacg
     1501 ccaaagtgat gggggcaatg atcttaaact ggcaggaaaa acgctggcag cgatttttta
     1561 acagcgaaga accgggaaat atcgaacccg tctacatgct ggaaaaagta gaaaatcaaa
     1621 accatgccaa atgggaggtc cataatttta ccatgggcta ccagcggcaa gtaaccgaag
     1681 atacttacga atatttgctg ttaaatggtg aggaatcttt taacgatctc ggcgagccag
     1741 agtggttatt ttcgcgcgcg ttgggtgtcg atatcccgct gacatcactt catattattg
     1801 gttaattaca aggacgttta caatgcatat actggattca cttcttgcct ttagcgccta
     1861 tttttttatt ggcgtggcta tggtgattat ttttcttttt atctattcta aaattacacc
     1921 gcacaacgaa tggcagttaa tcaaaaacaa taataccgcg gcttcactgg cgttcagtgg
     1981 tacattgtta ggttacgtta tcccattatc cagtgcggca atcaatgcgg tgagtattcc
     2041 agactatttc gcctggggcg ggattgcact ggtgattcag ttactcgttt ttgctggtgt
     2101 cagactttat atgcccgcat taagcgaaaa aattattaat cacaataccg cagcaggaat
     2161 gtttatggga accgccgcgc tggctggcgg tatttttaac gcagcttgta tgacatggta
     2221 atggacgatc atggccagaa aacgcaaatc aagaaataac agtaaaattg gtcacggagc
     2281 gatcagtcgc attggaagac cgcataatcc ttttgagccg cgccgcaatc gctacgcaca
     2341 aaaatactta acgttggtgc taatgggcgg tgccgttttt ttaatagtca aaggatggag
     2401 tgaaagtagc gatgtcgata acgacggcga cggaacgttt tactcgacag tgcaggattg
     2461 tattgatgac ggtaataatg ccgacatttg cgcgcgtggc tggaataacg ccaaagcggc
     2521 gttttatgcc gatgttccga agaatatgac tcagcagaac tgtcagtcta agtatgaaaa
     2581 ttgttactac gacaacgttg aacagagttg gatcccggta atatctggat ttttgttaag
     2641 ccgggttatt cgtaaagatc gcgatgagcc gtttgtttat aacagcggcg gttcctcttt
     2701 tgcttcgcgc ccagtctggc gcagcacttc tggtgattac tcttggcgct ccggttctgg
     2761 caaaaaagag tcttactctt cgggcggctt taccaccaaa aaagcgtcta ccgtttctcg
     2821 cggcggctat ggtcgttctt ccagcgcccg tgggcattgg ggaggctaat catgctgaga
     2881 cacaacgttc ctgtgcgacg ggatctggac cagatcgccg ctgacaacgg tttcgacttt
     2941 catatcatcg acaatgaaat ctattgggat gagagtcggg cttaccgttt tactctgcgc
     3001 cagattgaag agcagatcga aaaaccgact gcggaactgc atcagatgtg ccttgaggtg
     3061 gtggatcgcg cggtaaaaga tgaagagatc ctgacgcaac tggcgatccc gccgttgtac
     3121 tgggatgtga tcgccgaaag ctggcgcgca cgcgatcctt cgctgtatgg ccggatggat
     3181 tttgcctggt gtggcaatgc gccggtgaag ctgctggagt acaacgccga tacgccgacc
     3241 tcgttgtatg agtcggctta tttccagtgg ttgtggctgg aggatgcccg gcgcagcggc
     3301 attattccgc gtgatgccga tcagtacaat gccattcagg aacgcctgat ttcacgcttt
     3361 agcgagcttt atagccggga gccgttttat ttctgctgct gtcaggacac cgatgaagac
     3421 aggactaccg tgctgtactt gcaggactgc gcccagcagg cagggcagga gtcgcggttt
     3481 atctacattg aagatctcgg tttgggcgtc ggcggtgtac tgaccgatct tgatgataat
     3541 gtcatccagc gtgcatttaa gctgtatccg ctggagtgga tgatgcgtga cgataacggt
     3601 ccgctgctgt gcaagcgtcg cgagcaatgg gtggagccgt tatggaaaag tatcttgagt
     3661 aataaagggc taatgccgct gctttggcgc ttcttccctg gtcatcctaa ccttcttgca
     3721 tcctggtttg agggtgaaaa atcgcagatt gccgctggcg aaagctatgt gcgtaaaccg
     3781 atctactcgc gcgaaggcgg aaacgtcacc attttcgacg gtcagaataa cgtcgttgac
     3841 cacgctgatg gtgattacgc cgatgaaccg atgatctacc aggcgtttca acctctgccg
     3901 cgatttggcg atagctacac actcatcggt agctggattg tcgatgatga agcgtgcgga
     3961 atggggatcc gtgaagataa cacactgatc accaaagaca cctcacgttt cgttccgcat
     4021 tacattgctg gataagaatg ttttagcaat ctctttctgt catgaatcca tggcagtgac
     4081 catactaatg gtgactgcca ttgatggagg gagacacagt gcactggcaa actcacaccg
     4141 tttttaatca acctatacca ttaaataaca gcaacttata cctgtctgat ggcgcgctct
     4201 gcgaagcggt aacgcgtgaa ggtgctggct gggatagcga ttttctagcc agtattggtc
     4261 agcagttagg aacggctgaa tcccttgaac tggggcggct ggcgaatgtg aatccgcctg
     4321 aattattgcg ctacgatgcg caaggacgcc gtctggacga tgtgcgtttt caccccgcct
     4381 ggcacctgct gatgcaggcg ctatgtacca atcgggtgca caatcttgcc tgggaagaag
     4441 acgctcgctc cggcgcattt gtggcgcgcg cggcgcgttt tatgttgcac gcacaggttg
     4501 aggcagggtc gttatgtccg ataaccatga cctttgccgc cacgccattg ctgttacaga
     4561 tgttacccgc gccgtttcag gactggacca cgccgctgct gagcgatcgc tacgattctc
     4621 acttattgcc aggtgggcaa aaacgcggtt tgttgattgg catgggaatg acggaaaagc
     4681 agggcggttc cgatgtcatg agcaacacca cccgcgcaga gcgcctggaa gatggctctt
     4741 atcggctggt ggggcataaa tggtttttct cggtgccgca aagcgatgcg catctggtgc
     4801 tggcgcagac tacgggcggt ttgtcctgct tttttgtgcc gcgctttttg cctgacgggc
     4861 aacgcaacgc gattcgcctc gagcggctga aagataagct gggtaaccgc tctaacgcca
     4921 gctgcgaagt ggagtttcag gatgccattg gctggttgtt ggggcaggaa ggggaaggaa
     4981 ttcgtctgat cctgaaaatg ggtgggatga cgcgttttga ttgcgccctg ggtagccatg
     5041 ccatgatgcg ccgtgcattt tcgctggcga tttatcatgc acatcaacgc catgtttttg
     5101 gtaatccatt gatccaacag ccccttatgc gtcatgtctt aagtcgcatg gcacttcagc
     5161 ttgaagggca aacggcgttg ctgtttcgtc ttgcgcgagc gtgggaccgg cgtgccgatg
     5221 ccaaagaagc cctgtgggcg cgtttattta cgcctgcggc aaaatttgtg atctgcaaaa
     5281 gaggtatgcc gtttgtggcc gaagcgatgg aggtgctggg cggcattggt tattgcgagg
     5341 agagcgagct gccgcggctt taccgggaga tgccggtaaa cagtatttgg gaaggttccg
     5401 gcaatattat gtgcctggat gtgctgcgcg ttctcaataa gcaagcgggc gtatacgact
     5461 tattgtcgga agcgtttgtg gaagtgaaag ggcaggatcg ctattttgat cgcgcggttc
     5521 gtcgtttaca gcagcagctg cgtaagccag ctgaagaact gaggcgagag attactcatc
     5581 agctattcct gctgggctgc ggtgcgcaaa tgttgaaata tgcttctccg ccaatggcgc
     5641 aggcgtggtg tcaggtgatg ttagatacgc gcggcggcgt acggttgtca gagcagatcc
     5701 agaatgattt attgctgcgg gcgacggggg gagtgtgtgt gtaagcgtat acgactgatg
     5761 cgacgctggt ttcgattaac taaatgaaat atgtgaaaat tgtaggccgg acaaggcgct
     5821 cgcgccgcat ccggcattgt tcatttgctg tagatgacac tcatgcatac agtatcgcct
     5881 gtacgcgcca gttatcaggc tgctggtctt cgtacatggt aataatgcgg taccatgacg
     5941 caccttgttc atcggccttt aacgccacga tacgttccac gtcctgcgga tcccctgaaa
     6001 tattgctaac tgagatctga cctatttcat tcaggcccgt cacgcagtca gcactggcga
     6061 attctgcaga ctgtgccgtg gcgctcgcag gataagtcac taattgcagc gagggtgagg
     6121 caagtaattg tttcatcgtc agctccattt ttcgtttccc cacatgttgc agggcattcc
     6181 atcgcggtaa taaacgccca ggcttccagc cggtaattta ttgtgcacag gtaaacgcat
     6241 agggacgcaa agcaatgtaa agctgactat gtaagttgtc tgatttattt acggtacaaa
     6301 atggcttgtg aataccactg tcctgtcacg atggtttcgt ccaccatgac gacaacgtaa
     6361 taatcagctt ttgcagcgac agctttcgct ctgatttctg ctaatgcgtc atccggagaa
     6421 ccccgaacca tcgtgcttac gctgcctatt cgctgtaacc cttgcgtctg gtcgcggcga
     6481 atctcttgcg gatggtccgt tactggcggt gctggctgtg gcgtaccttg cagtgcgcta
     6541 caggcactta acatcaacac caataataaa ctggcaaacc ggtaaataac gctattacgt
     6601 ttcctgctaa ccatagtgta gtgccttatt aatttaactt tgggggaatg ttcccgaatt
     6661 gttacctggt acgcgatttt cgaatgaaaa cgtgacgaaa gcgtaaccca catctcaaca
     6721 ttatgaacta ataccgccag cagaaacttg tgctaaggat ttcttcatgt cataccagac
     6781 aaaaaggaga gacagatgat tgaaatagaa tcacgcgagc tggcagatat tcccgttctt
     6841 catgcttatc ctgtcgggca aaaagatacc ccgttaccgt gcgtaatttt ttatcacggc
     6901 tttacttcat ccagtctggt gtatagctat tttgccgttg cgctggcgca ggctggtttg
     6961 cgggtgatca tgccggatgc gcccgatcac ggtagccgtt ttagtggtga cgcagcgcgg
     7021 cggttaaatc aattctggca aatcttgcta caaagtatgc aggaattcac tactttacgt
     7081 gcggcaatag ccgaagaaaa ctggctgctt gatgaccgtc tggcagtcgg tggcgcgtcg
     7141 atgggcgcga tgacggcact gggaattacc gctcgccacc ccacggtgaa atgtaccgcc
     7201 agcatgatgg gatcgggcta ttttacatcg ctcgcccgtt cactgtttcc accgctgata
     7261 cctgaaacgg cagcacagca gaatgaattc aataacattg tcgcgccgct ggcagagtgg
     7321 gaagcgacaa accacctgga acaacttggt gacagacctc tactgctgtg gcatggcctc
     7381 gacgatgacg ttgtgcctgc cgatgaatca ctacgtttgc agcaggcctt aagcgagacg
     7441 ggacgggata aactgctaac ctgttcatgg cagccaggcg tgcgtcaccg cattacgcct
     7501 gaggcgttag atgctgccgt gacatttttc cgccagcatc tttaaactcg cagaatgctg
     7561 accccttgcg cttccagttg ttgcaggatt tccgggttag catttttgcc ggtgataagc
     7621 atatcgattt gatcggcacg gctaaaaagc attcccgcgc gttcgccaat cttactgcta
     7681 tcaaccagta ccaccagttt ccctaccacg ctcagcatct tctgctctgc cattgccgtc
     7741 agcatatcgg ttttatacaa cccttctgcg gtcagtcctt tgccgctggt aaacatccag
     7801 tgcccggcat agagactgtt ttcgctgccc tgcgggctta aagtgatgga ctgacttttg
     7861 ttgtactgtc cgcccataat gatcacgctg tcatgttctt gatcgatcag gtaatttgcc
     7921 agcggtagat aattagtgat gatttgcact ggcttgccac acatttcccg cccaagcaga
     7981 aacgcggtgg agccgcagtt gatgactacg ctttcgcccg gattaaccag ctgcgaggcc
     8041 gctttagcaa tacgtacttt ttcatcgtga ttctgcgcct gatgcagatt catcggcgtc
     8101 cagcgcgggc gctgctgggt aatagcttct gcgccattgc gcactttttt cagtttgccg
     8161 ctttcgtcaa gtttattgat atcgcgtcgc gcagtggcag gcgaaattcc cagacgctca
     8221 acgactttct caacggtcac aaagcccaat tgtgcgagca tttccaggag gatttgatgt
     8281 ctttgtgctt cagtcatgag ctattccgat aaaatttgat ttttttagat gatatttgaa
     8341 atagccagga aatactacgc cggatagcgc gaacttctcc acgctatccg gtataaacga
     8401 cagattacag gaatgacttg aacggcagat ccggttcaat agtgaagcaa tcatcgaaac
     8461 cgcgcggata gtggtactcg aagttgtctt tatccagcgg ccaggtaaat ttgccgccaa
     8521 cctgccagat aaacggcttg aagccatact tcagacgatc ttttttcatc tcccacagca
     8581 cgcggatctc ttgcggatcg gcctggaagt ttgaccagat gtcgtggtgg aacgggataa
     8641 ccactttcgc attcagggct tcacccatac gcagcatatc ggcgctggtc attttgtcgg
     8701 taataccgcg cgggttctca ccatacgatc ctaacgccac atcgatctga tgttcgttac
     8761 cgtgcttcgc ataatagtta gagtagtggg agtcgccgct gtgatacagg gagccgccag
     8821 gcgttttgaa cagatagttc accgcgcgat cgtccatgcc gtccggcagt acgccagccg
     8881 ctttttgatc ggcaggcagg gtgatcagtg cagtacggtc gaaagcgtca agcgcatgaa
     8941 tttcaatgtc tttcactttt acaacatcgc ccggtttgac cacgatgcaa cgctctttcg
     9001 gtacgcccca gccaatccac aaatcaacgc aggttttcgg tccaatgaat ggtacgtcat
     9061 cagcacagtt ctgcatcacg gcagcagcga cgttaacgtc gatatgatcg ttgtgatcgt
     9121 gagtcgccag taccgcgtcg atctggcgaa tcgcaaacgg atcaagaaca aacggggtgg
     9181 tgcgtaggtt tggctgcagt tttttcacgc cagccatgcg ctgcatctgg tgaccctgtt
     9241 tcattaacgg gttaccgtga ctttgtttgc cagtgccgca ccagaaatca acgcaaacgt
     9301 tggtgccacc ttctgatttc aaccagatcc cggtgcagcc tagccaccac atcgcaaatg
     9361 tgccaggggc gacctgctct tgttcaattt cttcattcag ccagctaccc cactccggga
     9421 aagtgctcag gatccaggat tctcgggtga tacttttcac tttactcatc gccatttacc
     9481 ttcatgatag ttcaattcga atcaatatgt gatttgtttt gattaatcct gacactattt
     9541 tttcaggaag gcaatgacca ttttttgact ttttgccagg gaagtggttg ttgattttga
     9601 gtgtggaaag atttaatgga atgtgtaatt caattaacta aatgaattta aatgaatagt
     9661 tgtttcgttg tgtgaatccc attctatcca tgtggaatta tttgcgggtc gcgtcacatt
     9721 taatcataaa taatcttgtt gtgattactt ttgaaaatta gagtgagtgc acaacattcc
     9781 gggtgtgtgg aatacccggt tacctcttct tcaggagatc gttatggaga tcctctacaa
     9841 catctttacc gtgtttttta accaggtcat gaccaatgcc ccgttgttgc tgggtattgt
     9901 gacctgtctg ggctacatcc tactgcgcaa aagtgtcagc gttattatta aaggcacgat
     9961 taaaaccata attggtttca tgttgttgca ggcagggtcc ggcatcctca ccagcacctt
    10021 caaaccggtg gtggcgaaaa tgtccgaagt ctacggcatt aacggcgcaa tttccgatac
    10081 ttacgcgtcg atgatggcaa ccatcgaccg catgggcgat gcctatagct gggtaggtta
    10141 cgcggtgttg ttagcgctgg cgctgaacat ctgttacgtg ctgctgcgtc gcattaccgg
    10201 tattcgtacc atcatgctga ctggtcatat catgttccag caggcggggc tgattgccgt
    10261 tacgttgttt attttcggct actccatgtg gacaaccatt atctgcacgg cgattctggt
    10321 ttcgctctac tggggcatta cctccaacat gatgtacaag ccgactcagg aagtgacgga
    10381 cggctgcggt ttctccatcg gtcaccagca acagtttgca tcatggattg cctataaagt
    10441 tgcgccgttc ctcggcaaaa aagaggaaag cgttgaagac ctcaaactgc caggctggct
    10501 gaatattttc cacgacaaca tcgtctccac ggcgattgtg atgaccatct tctttggtgc
    10561 cattctgctc tccttcggta tcgacaccgt gcaggcgatg gcaggcaaag tgaactggac
    10621 ggtgtacatc ctgcaaactg gtttctcctt cgcggtggcg atcttcatca tcactcaggg
    10681 cgtgcgcatg tttgtggcgg aactctctga agcatttaac ggtatctccc agcgcctgat
    10741 ccctggtgcg gttctggcga ttgactgtgc ggctatctat agcttcgcgc cgaacgccgt
    10801 ggtttggggc tttatgtggg gcaccatcgg tcagctgatt gcggttggca tcctggtcgc
    10861 ctgcggttcc tcgatcctga ttattcccgg ctttatcccg atgttcttct ccaacgccac
    10921 catcggcgtg ttcgctaacc acttcggcgg ctggcgtgcg gcgctgaaga tatgtctggt
    10981 gatggggatg attgaaatct ttggctgcgt ctgggcggtg aaactcaccg gtatgagcgc
    11041 ctggatgggc atggcggact ggtcgattct ggcaccgccg atgatgcagg gcttcttctc
    11101 catcggtatc gcctttatgg ccgtcatcat tgtaattgca ctggcttata tgttcttcgc
    11161 tggccgcgca ctgcgcgcag aagaagatgc agaaaaacaa ctggcagaac agtctgctta
    11221 ataaggagtt ttgattatga ccgtacgtat tctggctgtg tgtggcaacg gacaaggcag
    11281 ttccatgatc atgaagatga aagtggacca gtttttaacc caatcaaaca ttgaccacac
    11341 ggtaaatagc tgcgcggttg gcgaatacaa aagcgagttg agcggcgcgg atatcatcat
    11401 cgcttctacg cacattgcgg gcgaaatcac cgtgaccggc aacaaatacg tggttggcgt
    11461 gcgcaacatg ctctctcctg ccgactttgg cccgaaactg ctggaagtga tcaaagcgca
    11521 tttcccgcag gatgtgaagt aaggacgcgc catgaaatta cgtgattcgc tggcggagaa
    11581 taaatccatc cgcctgcagg ctgaagctga gacatggcag gacgcagtaa aaatcggcgt
    11641 tgacctgctg gtggcggcgg atgttgtcga gccgcgttac taccaggcga ttctggatgc
    11701 ggttgaacag cacggtccgt actttgtgct cgcaccaggc ctggcaatgc cgcacggtcg
    11761 cccggaagag ggcgttaaga aaaccggttt tgcgctggtg acgctgaaaa agccgctgga
    11821 gttcaaccac gaagataacg acccggtgga tatcctcatc accatggcgg cggtcgatgc
    11881 caacacgcac caggaagtgg gcatcatgca gatcgtcaac ctgtttgaag atgaagaaaa
    11941 tttcgaccgt ttacgtgcct gccgtaccga gcaggaagta ctggatctca ttgaccgcac
    12001 caacgcggca gcttaagaag gaattgaaga tgtcattacc gatgttgcaa gtcgcgctgg
    12061 acaaccagac tatggatagc gcctacgaaa ccactcgcct gattgccgaa gaagtcgaca
    12121 ttatcgaagt gggtaccatt ctgtgcgtgg gcgaaggcgt gcgtgcggtt cgtgacctga
    12181 aagcgctcta cccgcacaaa atcgtactgg cagacgctaa aattgctgat gcgggcaaaa
    12241 tcctttcgcg tatgtgcttc gaagccaacg ctgactgggt gacggtaatt tgctgtgcgg
    12301 atatcaacac cgccaaaggc gcgctggacg tagcaaaaga gtttaacggc gacgtgcaga
    12361 tcgaattgac cggctactgg acctgggaac aggcgcagca gtggcgtgac gcaggcattc
    12421 agcaggtggt ttatcaccgc agccgtgacg cgcaggccgc aggcgtagcg tggggcgaag
    12481 cggacattac cgcgatcaaa cgtctttccg atatgggctt caaagtcacc gtcaccggtg
    12541 gcctggcgct ggaagatctg ccgctgttca aaggtattcc gattcatgtg tttatcgctg
    12601 gtcgcagcat ccgtgatgcc gcttctccgg ttgaagctgc acgtcagttc aaacgttcca
    12661 tcgccgaact gtggggctaa ggagcggata tgttgtccaa acaaatcccg cttggcatct
    12721 atgaaaaagc gctccccgcc ggggagtgct ggctggaacg cctgcaactg gcaaaaacgt
    12781 tgggcttcga ttttgtcgag atgtcggtag atgaaacgga cgagcgcctg tcgcgcctcg
    12841 actggagccg cgagcagcgt ctggcgctgg tgaatgcgat tgttgaaacc ggcgtgcgcg
    12901 tgccgtccat gtgcctttct gctcatcgtc gtttcccgct gggcagtgaa gatgacgcgg
    12961 tgcgggcgca ggggctggag attatgcgta aagctatcca gttcgcccag gacgtcggca
    13021 ttcgcgtgat ccagctggcg ggctatgacg tttactatca ggaagccaat aacgaaacgc
    13081 gtcgtcgttt ccgtgacggc ctgaaagaga gcgttgagat ggcgagccgc gcgcaggtga
    13141 cgctggcgat ggagatcatg gattatccgt tgatgaactc catcagcaag gcgctgggtt
    13201 acgcgcacta tctcaacaac ccgtggttcc agctctaccc ggatatcggc aacctgtcgg
    13261 cgtgggacaa cgatgtgcag atggagttgc aggccggaat cgggcatatc gtcgcggtac
    13321 atgtgaaaga caccaaacct ggcgtcttca aaaacgtgcc gtttggcgaa ggtgtagtgg
    13381 atttcgaacg ttgtttcgaa acgctcaaac agagtggcta ttgcgggccg tacctgattg
    13441 agatgtggag cgaaacggcg gaagacccgg cggcagaagt ggcgaaagcg cgtgattggg
    13501 tgaaagcgcg catggcgaaa gcgggcatgg tggaggcggc ataatgcaaa agctaaaaca
    13561 gcaggtattt gaagccaaca tggatctgcc gcgctacggg ctggtgacct ttacctgggg
    13621 caacgtcagc gctatcgacc gcgaacgcgg gctggtggtg atcaagccca gcggcgtcgc
    13681 ctacgaaacc atgaaagcag acgatatggt ggtggtcgat atgagcggta aggtggtgga
    13741 aggggagtat cgcccatctt ccgacaccgc gacgcatctt gaactctacc gtcgttatcc
    13801 gtcgcttggc ggcattgtcc atacccactc cactcatgct accgcatggg cgcaggcggg
    13861 gctggcgatc ccggcgttag gcaccacgca cgctgactac ttctttggtg acatcccgtg
    13921 tacgcgtgga ttaagcgaag aagaggtgca gggtgagtat gaactgaaca ctggcaaagt
    13981 gattatcgaa acgctgggca acgccgagcc gctgcatacg ccgggaattg tggtgtatca
    14041 gcacggaccg ttcgcctggg ggaaagatgc ccacgacgcg gtgcataacg cggtagtgat
    14101 ggaagaagtg gcgaaaatgg cgtggattgc gcgcagtatt aatccacaac tcaatcacat
    14161 cgacagcttc ctgatgaata aacacttcat gcgtaagcat ggtcctaacg cttattacgg
    14221 gcagaagtaa aacacgcgct gcggaaattt ccttcttcgg gagataactg gtctaattcc
    14281 gcagccgttt ttcaaaaaaa gccccctgcg aagggggcaa agcaaactat ggcaatgttt
    14341 cgttggttat acctggtgct agcgataaat atccgcgctg gcgtgcatat tgccgttact
    14401 ccccggttcc cgcatcagaa ttacgtggta gtacgttgcg ccctgcgcat ctgtttcttc
    14461 atttaatgcc tgacgtgctt cactttcagt ggcgaaatta tgattgatat aaatcacgcc
    14521 taagctttgc acatcgtcca tatttctggc ctggtggtta ttgatttcaa tggctgccca
    14581 tgtatttgca cttagcaaaa gcacagccag aagggctaaa actcgactga acatagatac
    14641 ctcctcgacg gctgactttg tgtgctctcc tctgtgatga tcttctgatt taattttaat
    14701 caatgataaa gaagttgatg gtgaccattt ctgatgcagt tgttcaaaaa aacaccatga
    14761 tgaagtgtga tgaactccaa atcagcggtt tagaggttaa ttgcgaaagg ggagatttat
    14821 ttcggctctg cccttgagtt tagcggggca tacaagtact ataacgcgtc atttttcagc
    14881 cgacctttaa cacgttcctt gcctccccgg gattcggctg acccagacag gaggctgaat
    14941 aatccgtaag gagcaattcg atgcgtcatt acgaaatcgt ttttatggtc catcctgacc
    15001 agagcgaaca ggttccgggc atgatcgagc gctacactgc tgccatcact ggtgcagaag
    15061 gcaagatcca ccgtctggaa gactggggcc gccgtcagct ggcttacccg atcaacaaac
    15121 tgcacaaagc acactacgtt ctgatgaacg ttgaagctcc gcaggaagtg atcgatgagc
    15181 tggaaactac cttccgcttc aacgatgccg ttatccgcag catggttatg cgtaccaagc
    15241 acgctgttac cgaagcatct ccgatggtta aagcgaaaga cgagcgccgt gagcgtcgcg
    15301 atgatttcgc aaacgaaacc gctgatgatg ctgaagctgg ggattctgaa gagtaatttc
    15361 tgatgaccaa ccgtctggtg ttgtccggca ccgtgtgcag gactcccctt cgaaaggtca
    15421 gtccatcagg aattcctcac tgccagttcg tgcttgagca tcgttctgtg caggaggaag
    15481 ccggctttca ccggcaggcg tggtgccaaa tgcccgttat tgttagcgga cacgaaaacc
    15541 aggccattac tcacagtata acggtcggca gtcgcataac cgttcagggg ttcatttcat
    15601 gccacaaggc aaagaacgga ctgagcaaaa tggttttgca tgccgagcag attgaattga
    15661 tagattctgg agactagcca tatggcacgt tatttccgtc gtcgcaagtt ctgccgtttc
    15721 accgcggaag gcgttcaaga gatcgactat aaagatatcg ctacgctgaa aaactacatc
    15781 accgaaagcg gtaagattgt cccaagccgt atcaccggta cccgtgcaaa ataccagcgt
    15841 cagctggctc gcgctatcaa acgcgctcgc tacctgtccc tgctgccgta cactgatcgc
    15901 catcagtaat cggtcacggt ccattaatac gactttgaga ggataaggta atgcaagtta
    15961 ttctgcttga taaagtagca aacctgggta gcctgggtga tcaggtaaac gttaaagcgg
    16021 gctatgctcg taacttcctg gtaccgcagg gtaaagctgt tccagctacc aagaaaaaca
    16081 ttgaattctt cgaagcacgt cgcgctgaac tggaagctaa actggctgaa gttctggcag
    16141 cagctaatgc tcgcgctgag aaaatcaatg cactggaaac tgttaccatc gcgtctaaag
    16201 ctggcgacga aggtaaactg ttcggttcca tcggtactcg cgacatcgct gacgctgtaa
    16261 ctgcagctgg cgttgaagtg gctaagagcg aagttcgtct gccgaacggc gttctgcgta
    16321 ccactggcga acacgaagtg agcttccagg ttcacagcga agtattcgcg aaagtgatcg
    16381 taaacgtagt agctgaataa ttcgttattc aacgagacgt aaaaacgccg accattggtc
    16441 ggcgttttgc ttttgtagcg agcacttgca gatatcgata agggccacaa taagcagccc
    16501 tacgaagata attacttagc tgcctgaata tcagccagca ttggggcaag ggctgggttg
    16561 tccttagcga agtttgcgta cattggctgt actttctcga taaacggagc tttatctacc
    16621 tggtagactt tcacgcccat tttatccagt tttgcaaatt cttgtttctc ttcctcgctc
    16681 cacaagtctt tcatgtagct cattgattct ttagctgctt ttaatataac tgcctgattt
    16741 tccgggctaa gtttatcaaa cgcagcattg ctcatcagaa ccacatcggg aaccatagtg
    16801 tgttcgtcaa ggctgaagga ttttgccact tcaccgtgac gcatggtagt cagcgccatc
    16861 acgctgtttt ctgccatatc gaccacaccc tgctgtaagg ctgtatacaa ctcgccgtaa
    16921 tccagtggcg tcgggttacc gcccatgact ttgatcattt caactgcgcc agggcttggc
    16981 tggacacgga ctttcatgcc tttcagatcg tctggtttca gtactggttt attgccatag
    17041 aaactgcggg cgcctccatc ataaaaagtc agcccgaaat aacctttgct tttcgttgag
    17101 tcgaggattt ttctcccgat atcgccctgc atgacctgat aataatgatc acgatcgcgg
    17161 aacaaataag gcaggctaaa taatttatag gaattttcaa atgcttccat ttctgccgca
    17221 ttggttttta ccaggggaat agcgccagaa cgaatcagct ccattgtttc tcgttgagta
    17281 cccaatgttc catttggata aatacgaata gtaatatcac cgttagagta ctctttgctc
    17341 tttttcgcaa agaactccat cgctttatga acaggatgag acttatcctg attatgactc
    17401 agttttaatg tcactgccca ggactggctt gcggataaca taagaacagc accgataatg
    17461 ccagcggata cttttatttt catagatgtt ccttttctaa taaatatggc ggcaggtgcc
    17521 gccagtgaat gcgcatcagt agcccagtgc tcttggcaat gccatactga tttgcggaat
    17581 ataggtcacc attgccagca ccaataccat cacggcatag aatggcagca taggtttgat
    17641 aattttatta atcggtaatt tagatacact acaaccgaca aataacacac tgcctacagg
    17701 cggagtacaa ataccgatac acatgttcag taccatgaca ataccaaagt gaatcgggtc
    17761 catgcccagt tcggtgacaa ccggtaaaaa tatcggcgta aaaatcagta ccgccggtgt
    17821 catatccata aaagtgccga taatcaacag gataatattg ataataagca ggattattat
    17881 tgggttgtca gaaatagtgt taagcaaatc gagaatcagg aagggaacgt cagcgtttga
    17941 cattgcccat gacataccca tcgaagaacc aattaataac aagacaattg cagtggtaat
    18001 aacggattcc agaaatattt taggtaaatc tttaactgag atttcacgat aaagaaccag
    18061 tgcaaggaaa agggtataaa ttactgcaat tgcggaggcc tctgtcgggg taaaaatacc
    18121 acttaataca ccccccataa ttaaaataat caggaaaata ctggggatag ccatccatat
    18181 cgcaacgcca gcctggcgta aagtcgggcg ttctggcttt ggataaccgc gacgtaccgc
    18241 gataataccg gctattacca tcagtgccag acccagcaaa attcccggaa cataacccgc
    18301 caggaacagt gcggcaatag aagtaccacc actgaccagt gaatagacga ttaacgtatt
    18361 acttggtgga ataagcaaac ccgtcggggc agaggcaacg ttaaccgcag tactgaatgc
    18421 cgggtcgtaa ccctctttaa cctgttgcgg atgcatgacg ccgcccatcg cagccgccga
    18481 agccacggct gaaccagaaa ttgccccgaa cagcatattg gcgagaatat tacagtgggc
    18541 aagtgagcct ggcagacgac cgccgagaat acgggcaaag ttgattaatc gtcgcgctat
    18601 tcctccatgg ttcataatgt tgccagacaa gataaaaaat gggattgcca acaacgtaaa
    18661 actatctaac ccggaggcta acttttggga aaccacactc aatgctgagt tgaaatcgag
    18721 ttgtgtggaa attgatgcca gtgtcgataa accaatggca taaacaatag gtacacctaa
    18781 tgccaacata aaaaagaagg caccaaataa cacaataatc ggtaaccagt aatccataat
    18841 ttctccattc ctactatggt tggttgttgt ttgttatctt tagagtggtt gtttaagttc
    18901 ggttaaaacc atatctatgg tgtatataat aataagtaca ccactaatcg ggatgctcat
    18961 atagatatat cccatttgcc agcctaaagc gggcgaggtc tgacctgaat cgaatgtatc
    19021 catcgttaat agcccaccac catataccat aatgatggtt gcgaagataa taacgataat
    19081 ctgaatacac aaaaataaat gacgtcgggc ttttcctttc agcatcatag gtagcagatc
    19141 aatcgccaga tgccggtttt gccctgcggt gtatgcccca ccaaaaagtc caagacaaat
    19201 aaagagcaaa cggctgagtt catctgtaaa aatactggga ctagtaaata taaatcgtgc
    19261 agccacctgc cacgtcacac atgccacgat gatcgccaaa atggtaatgt tcaatgctgc
    19321 aagtagagca ttaatagatc taattatatt tcgcatagtg attaaccttt gcaatatcat
    19381 tcatcctgtg gctggtaaat tgtcccatta agaaaaccag gccagttttt ttcaaaccaa
    19441 atacctcgga taataggaaa ctcacgaaca ggtgtttctc tgacgtgaaa gataccgttg
    19501 caataatgaa taagtagttt atctttttta tcctcagtac gctctgaacg aaagcgccag
    19561 gcatcacaaa aaccatttcg tgtttgcgga atagaatttc catccctgtc gagaataata
    19621 cgtgctcccc gactaccacc acctgcgtca atataatgcg ttaattgcgt taatacggcg
    19681 gcagaagtca gtgccatatg ccgccacata aacagctcgg caacttcacc cacatgtttg
    19741 atagccaatc catgccgttg gacaaattcg ctcaatagca gggcatcgcg agtggcgcgt
    19801 cggactttat cggcatggca aataaatccc gcatggtcag acattcgtgc ctgaattttt
    19861 tctctcacaa ccgacaacgg catcccggtc ccgttatcgt gcgcctgagt aatgatctct
    19921 cttatagaag ccagtgtagg agctaccagc tgtgctatat ctccattaat attccgcttt
    19981 tgcgtgcaac caataaaacg tgccagacga acagcaaaaa cctgcccggc attcaatgct
    20041 gcaccaccag ggcgagtgac gccgtgtgtg ccagcaactt cccccacggc gaaacagccg
    20101 ggcagggagg tttgtcccca gatatccact tcaatgccgc cattcatatg ctgattattc
    20161 atggcaaact gcaaaggctg tgtggtgaga tcgtaaccgt gcatcttata cagcgagata
    20221 gacagcggat tcattcgttg cagccgttcg atgggcgatg gtgccagagc gtcgttattt
    20281 tccagatacg cgcgaacgtc gtcgtccagt cgatctaatg agaacggcag gtcacccggc
    20341 accgcttcag gattgcgatt gaaatccata aatacctgac gccctgattg ctgctcttgc
    20401 gctactgcca tatctaacaa gctggagcca aaatccatca cccgcgtggc gtggaacggc
    20461 cactgatagc ctttacggaa aatgtttgaa gccagctcct gggtggtgcg atagtaatcc
    20521 gcgaggaagt tatactcgtt accctcagca tccacggaat agatatacgg gatcacctgt
    20581 acataggtgc cggataaatt ccacggaaac gtgctacgcg gcgtgcctat accaaactgg
    20641 ctttcggtca gattggttag cgtcagacct tcctccagcg ccagccccag cgagccgaag
    20701 catttatgtg gataaacact gtcgcgataa agctcgccag gccctcctgt tgccagtacc
    20761 acattgggcg cagtcacaat tgccagcccc catgggttat gggcgcgatg accggtcgcg
    20821 aggattgccc ctgccacgcg gtcttcgccg ttttcgtcac gctgatgcag cagtttaatc
    20881 actgttgcac tggtcaacac tggaatggcg aggcgctgta cttcttccaa cagcactttc
    20941 accattaacc ttgaggtacg cggcccacac gaggttgcac gcccggcttc gtcatggtcg
    21001 gtttgataac gaagaatcgc gccatagcga tcttccggta attccagacc aagatattgc
    21061 aacccgccta gagtgtgcag agaacctacc gcttcgacat aagcggtgtc gtgatccatc
    21121 gccccaccgc tcgccagtgc ctctgccagt ttggtgaagt tgtcgccgtt gcccgcggta
    21181 gcggcggtaa atagtgtctg tttatcggaa ccagaacatg ccgacgtccc catatataac
    21241 ccggcggtag cgatcagcac attttgctgg cggcgtttaa gttcaactgc cgcacgtaat
    21301 cctgctgcac cactgccgac taccagggca tctgtttgcc agagtgggta atcaacaccg
    21361 tctaccgtga caacccatgc gggctctgcc tgtgcaaaac cctttgggaa agtaacgtgc
    21421 gtcagcgcat caaggatggt gtcatcaagc tgactcatgc atcctccatc ggcgtaaaca
    21481 ggctactttc catcaggcgt gggtgcttga taattggacg gaaagccata tacggcagaa
    21541 tgtcgcgctc aatctccaca cccggcgcga tttcaattaa ttcaacgcct tctgccgtta
    21601 actggaatac tgcgcgttca gtgatataca acacttcctg accactttgc tgtgcgtagg
    21661 cggcgctaaa agtgattttt tccacctggg taaccagttt agggatttga cctgactggg
    21721 tgatatgcaa gccatctggc gttacatcaa tcttgctacc tttggcgtcg aacgtgccgc
    21781 agaacaccac tttacgggcg ttttgcgcaa tttcgagaaa accgccggga ccaatgagat
    21841 tgccattgag atgggatacg ttgacgttac cgtactgatc catctctccc atgccgagga
    21901 aggtgatatc gatccctccg ccactgtaga actcaaattg atcgagtgat ggaataatgg
    21961 catcggcgtt gcgggcgcag ccgaaaaacg catcatccag catcataccg ttatgcacgc
    22021 cttgctcgac gctcagccat aaactttggt aaggcacgcc ttcgcgcagc gctacgcctg
    22081 gaataccccc aggaatacca aagccaaagt tggttgaagc gccagctacc agttcgcgtg
    22141 ccgcccgacg ggcaatcagg cgacgtaccg gatgactaac cagttcaatg gcgtcgttag
    22201 aactcagacg gcgatgttgg ccgctgatgg tcaggtcgta accgccaaga taggtttgtg
    22261 gttgttcgcg gtgttcaacg atgccatcaa ccagaatgcc cggtaatttg acccggcgtg
    22321 gttcaatcgc gccagcttcc agcacatcgc gtacctgtac gaacactttg ccgccgctgt
    22381 tgtgtgctgc cagcgccatg gaatagctat ccatatcaat cgcttcttct tcaaggctga
    22441 cgttgcctcg aggatcggca taagtgccac gcaaaatcgc gtaatccact ttgaaagggc
    22501 gataacgaag tttggtttca ccgtcgattt caatcagttc taccagctcg tcggtagtgc
    22561 attcattcga cttaccgccg ccattgcgtg gatcaacaaa cgatcccagc ccaacgtggg
    22621 taaaaagccc cggacgtccg gcgccgatct cccgtagcag tgcctgaatc acgccaccag
    22681 gaaagcagta ggcttcaatc gtgttatttt ttaccagcgc ctgcattttg ggcgaccagg
    22741 taaagtgccc ggcaataatg cgtttgagca tctctgcgtg cgcaaaacgg ttagtgcctt
    22801 tgcagtcgcg atcgccaata cccagcgagt ggattagcgt caggtagcga gggtgtccgg
    22861 tttgcaggaa acgcgcttca atagcagcca gaatatggtc ggcttccacc ataccgccgc
    22921 cgttaccgct aatagcaata gtcgcaccgt cctgaatttg cgccgccagt gcctcggcgg
    22981 ttgttatctt acgcatacaa tttccttatt gattatggaa gctcacgcta cttttttcgg
    23041 caaaggcgcg cgtcgcctgc tggcaatcct gagtggcaga cattaatccg gccagtagct
    23101 ggtgcgggta atcggcatgt ttattttcca gtgccagaat cagttgctta atatgcgcca
    23161 gtgccaccgg accgcatttt tccagctgcg ctaactggtt cgccatccag ctttccacct
    23221 gttctttttc taccacttca tcaattaaat tcgccgcctg ggcttcctgc gccgtcaggc
    23281 gtttacccag catcagcatc tggcgaccga ccaccggacc aacctgatta agcacacgtt
    23341 caatgcccat ccagccaggc accatgccga gcatcacttc cgggttagag aatttcgcgc
    23401 cggggcgggc aatacgaata tcgcagcaca aggccagttc aataccaccg ccgatggtat
    23461 gaccgttgag attcgcaacg gttaattggg gtatgtttcg caggcgatta aaaacgtcat
    23521 taccgcgttt aatccatttt ctgcccatat ctaatggcga ataggccgac cacgatttaa
    23581 tatcgccgcc cgcacaaaag aatttatcgc cggttgccgt taattcgact aatcgtaatg
    23641 tggtgtcgtt ttcaatttca tttaaataat ggtcgagaga ttcgatcatc tcttcgttaa
    23701 tagcatgaca tttatcttca cggttcaatg tcaggcggca ggaagccgcc actctgctga
    23761 acagaacggg ttggtcactc atgatcattc cttaacaata atgcgcgatg cgatcgacgc
    23821 taatatccag cgtgctgtta atatctttct gccaccagta aggcgagaat atttccagtt
    23881 caatagcacc attaaatccg gcgttttcaa ccagccgacg aattgacgga atattgatga
    23941 cgccatctcc cggcatacca cggtcattga ccagatcagt ggtcggcact aaccagtcgg
    24001 aaacatgaaa cgcgaggatg cgttttccgg cacgcaggat ctgactggcg agatccggat
    24061 cccaccagac gtgataaaca tccaccgcca cgccgaggcc aaactcacca tcgggatcga
    24121 gttcatcaca ccagtccagc gcctggcgta acgtgcacag gcaagagcga tcggcagctg
    24181 tcatcgggtg cagtggctcc agcgcaattg gcacgccgac atctttcgaa tgtggcagca
    24241 actggcgaat cccttgtttt acttgttccc gcgcttcgta cagatctttt gtgcccgttg
    24301 gcagaccgcc aaccacctgc atataacagg cggcgttaag caccgctgcg tcgtccagcg
    24361 cccggcggtt atcatcaatc gccagtttgc gttccgccag tgtcggcgcg gtgtagtaag
    24421 tgctacggca caaaccggaa acgctcatgt tactggcggc aagctggtgg gcgatttgct
    24481 ggagatcttc accttgcaac tccctgcgcc agggagcaat cgcgccaata ccacgcgcgg
    24541 cgcaggcatc aataatggcg ggtagcggcg ttttatagcc cagtgttgcg gtattgatac
    24601 tgaataaatc cgggcgttgt aacagatctc tcatgattca gcctcagaag cgcggcatac
    24661 ttaagccgcc gtcgatggcg accgcctgac cacaggtgta aatcagttta ccttccgcca
    24721 tcgcgcggac agtggaagca atatcagccg gataacccca acgtccccac ggcaccagac
    24781 ctttggcaat cagttcgtcg taataagccg ttgccggaat agtcatatcg gttttgatga
    24841 gaccggggcg cacttcataa acgccaattt gttcattaca aaggcgagca gcaaacagac
    24901 gagcagcggc agaaacggcg gttttggcaa tggtatattc gccgcgattc atcgccagca
    24961 tgatggcgtt gatggaactg acgaaaataa tcgagcggtg cggcagttct gcttccggtt
    25021 tcggctgtgc gagcagacgt ttactgaatg cctgagcgag gaagaacggc gcgcgggtgt
    25081 taatggcaat attttggtcg aagctgtctg gttcgaggtc gagaaggtca ccgcgttttt
    25141 tcactgaaat gcctgcgttg ttgagcaggc agtccagacg tccccactgg ttttgcgccg
    25201 cgtcgagcat ctcttcatgc aaggagagat cgccgacatc ggcagggaag caaatcactt
    25261 ccacgccttc agctatacat tcttgttgtg tgatatgcag tttttctacg ctgtcggcat
    25321 cgggaagatc gttaatcagc aggttaaatc cgccacgagc aagctccagc gcacagccct
    25381 ttccaatacc acgagcggct ccggtaatca cggcaacagg acgagtggat ttcatacaat
    25441 agctctcttt ttttgaatat tgttttctaa gtgacaatta gcttgcgacg tgccgcaaga
    25501 aagatcaacc atctggttgg ttgatatgcg atagtgatca cagtacattt gataacttga
    25561 tgaaagtaca agtttttatc ctttttactg gagcttctaa ttaatcgggt taagatagtt
    25621 atcttgaaga ggtcgtgggg aagcgttatg tatcttgata ccagcaacgt acagagcctg
    25681 aaggaaaagc tgctgttatg cgcggttaat gaatttgccg aatatggtta tgaaggggcc
    25741 agggtcgata atattgttaa ggctgcgggg tgtagtaaac agacggttta tcatcacttt
    25801 ggtaataaag aaaatttatt tattgaagta ctggaatata cctggaacga tattcgacaa
    25861 aaagaaaagg cccttgattt ctcagatctt ccgccacaaa aagccattga gaaaattatt
    25921 gattttacat gggattatta tattgctaac ccctggtttt taaaaattgt gcatagtgaa
    25981 aatcaaagta aaggtgtgca ttatgcaaaa tcacagcgtc tcttagaaat taaccatgcg
    26041 cacttgcagt taatggaatc cttattggat gaagggaaga aacacaatat ttttaagcca
    26101 gatatcgacc cattgcaggt gaatattaat attgctgcgc ttggcggata ttatttgatc
    26161 aaccagcata cgcttggcct ggtttatcat atcagtatgg tttcccccca ggcactggaa
    26221 gccagacgta aggtcatcaa agagacaatc cttagctggc ttttggttga cccttcatct
    26281 accgcgcacg aataaaactg ccatccggct ggcgggtaaa caggacctgt tgattattcc
    26341 ccgtatcaat ggttaagccc gtcaccacgc cgctggcgtt ctggcggatt ttcaccattt
    26401 gcccgttttg caaattactc agcggcttac ccgcgccttc tacctgcgcc attgcataga
    26461 catcggtagc gggcagtccg tggtcgcgga acagctgcgc catcgtttta ccgggttcga
    26521 cacggtaaga gcgccattga ttatcaattc cgctatctgg ctggaatggt tgcgtttggg
    26581 tagtttgcgg ctgctcttct ggctggcctt cctgtatagg ttctggagcg accggagcca
    26641 cctgatcagg atcattttgt ggggtgacta attgcgcccg cagctgttct tctgtcggcg
    26701 gttgtgattg cgactgaata tctaactgcg cttcacgcgt caccaccggc gcgttgggca
    26761 tatcatcaga tggaagcaga aatccgacca ccaatacaat ggcggcaata atgatccccc
    26821 gacgatgcat cggcggcagc gggtccatga agcgaaaatt gtccggcgcg tgccagactt
    26881 tctccagggt tggttttagt tcaaagcgcc cgggcatggt ttcctcctgc tccgcgtctt
    26941 gttcctcaat catagcctat gaataagcta acgctatgat gtccgtggta aacccgcctt
    27001 tattatattc atacgggata ttgctattgt ttctttttcc ccgggatttg tcatcattcc
    27061 tgcgacaaag ttttacccaa agaagtgtgg ctgatatgct gcccgctact tttataccct
    27121 aagaaaggaa atacgatgac caccccaact tttgacacca tcgaagcgca agcaagctac
    27181 ggcattggtt tgcaggtagg gcaacaactg agtgaatctg gcctggaagg gctgctgcca
    27241 gaagcactgg ttgcaggtat tgccgatgcg ctggaaggca aacatccggc tgttccggtt
    27301 gatgtggtgc atcgcgcgct gcgtgaaatc cacgagcgcg ccgatgccgt tcgtcgtcag
    27361 cgtttccagg cgatggctgc tgaaggtgtg aaatacctgg aagaaaacgc caaaaaagaa
    27421 ggtgtgaata gcaccgaatc tggcctgcaa ttccgcgtga tcaaccaggg tgaaggcgca
    27481 attccggcac gtaccgaccg cgttcgtgtt cattacaccg gtaaactgat cgacggcacc
    27541 gtgtttgaca gctccgttgc tcgtggtgaa cccgctgaat ttccggttaa tggcgtgatc
    27601 cctggctgga ttgaagcact gactctgatg ccggtaggtt ctaaatggga actgactatc
    27661 ccgcaggaac tggcatatgg cgagcgcggc gcaggcgcat ccatccctcc gttcagcacc
    27721 ctggtgtttg aagtcgaact gctggaaatc ctctaagcag cgcattctgt tcccctcgaa
    27781 ccagagggga gcaggcattc agcaataaac ccttcagttt gccaaacggc gctattttgt
    27841 gttgcaaaga ccccgtaagc gtgtattttt gtgagctgtt tcgcgttatc accgtgatat
    27901 gacactcact ttaaacataa aattaacatt agatctaaat cttagtattc atcccgcgta
    27961 ttgttaccta atatcgatga gtcccgatac agattcgtcg tatcatagac tgactaaagg
    28021 ccgtagagcc tgaacaacac agacaggtac aggaagaaaa aaacatggta gatcaggtaa
    28081 aagtcgttgc cgatgatcag gctccggctg aacagtcgct acggcgcaat ctcacaaacc
    28141 gacatattca gcttattgcc attggcggtg ccattggtac ggggttgttt atggggtctg
    28201 gcaaaacgat tagccttgcc gggccgtcga tcattttcgt ttatatgatc atcggtttta
    28261 tgctcttttt cgtgatgcgg gcaatggggg aattgctgct ttcgaatctg gaatacaaat
    28321 cttttagtga cttcgcttcc gatttactcg ggccgtgggc aggatatttc accggctgga
    28381 cttactggtt ctgctgggtt gtaaccggta tggcagacgt ggtggcgatc acggcttatg
    28441 ctcagttctg gttccccgat ctctccgact gggtcgcctc gctggcggtg atagtgctgc
    28501 tgctgacgct caatctcgcc accgtgaaaa tgttcggtga gatggaattc tggtttgcga
    28561 tgatcaaaat cgtcgccatt gtgtcgctga ttgtcgtcgg cctggttatg gtggcgatgc
    28621 actttcagtc accgactggt gtggaagcgt cattcgcgca tttgtggaat gatggcggct
    28681 ggttcccgaa aggtttaagt ggcttctttg ccggattcca gatagcggtt ttcgctttcg
    28741 tagggattga gctggtaggt acaaccgctg cggaaaccaa agatccggag aaatcactgc
    28801 cgcgtgcaat taactctatt ccgatccgta tcattatgtt ctacgtcttc gcgctgattg
    28861 taattatgtc cgtgacgccg tggagttcgg tagtcccgga gaaaagcccg tttgttgaac
    28921 tgttcgtgtt ggtgggtttg cctgcggctg ccagcgtgat caactttgtg gtgctgacct
    28981 ctgcggcgtc ttccgctaac agcggcgtct tctctaccag ccgtatgctg tttggcctgg
    29041 cccaggaagg tgttgcaccg aaagcgttcg ctaaactctc taagcgcgca gtacccgcga
    29101 aagggctgac cttctcgtgt atctgtctgc tcggcggcgt ggtgatgctg tatgtgaatc
    29161 ccagcgtgat tggcgcgttc acgatgatca cgaccgtttc cgcgattctg tttatgttcg
    29221 tctggacgat tatcctttgc tcgtacctgg tgtaccgcaa acagcgtcct catctgcatg
    29281 agaagtcgat ctataagatg ccgctcggca agctgatgtg ctgggtatgt atggcgttct
    29341 ttgtgttcgt ggtcgtgttg ctgacactgg aagatgacac tcgccaggcg ctgttggtca
    29401 ccccgctgtg gtttatcgca ctggggctgg gctggttgtt tattggtaag aagcgggctg
    29461 ctgaactgcg gaaataaccg cattatcatg ctggatggcg caatgccatc cagcttttag
    29521 attactcacc cgccagcgcg cgtgggaaca gtacattgtt ttccaggctg atgtgatcca
    29581 tcaggtcatc aatcagttca ttaatgccgt tatacatcgc tttccaggtg gtgcaggctt
    29641 ctggcggcgg tgtgacgtta ttggtagtgt gtttgatcac ttccagcagt tcgcccgctt
    29701 catcgtgctc gctttccatt acgctgattg gccccattgc ctggctgccc atgccttgtt
    29761 tgatcatcgg gaagaggatc tgctcttctt tcatcatgtg gctggaaagc tcttcatgca
    29821 gcatagtcag gtattttgtc agcccttttg gcacgctcgg tttgtcggcg tgaacgcgct
    29881 cgactttagt cgcttgcagg atcagctccg gcagctgctc gcggtgacga tcgtggtagc
    29941 gcacgatgat atggtcaatg atttctgcca gcggggcgct acgccagtct ttctcaatcg
    30001 gttgttcagc aagctttgcc agttcagctt caatgacctc aacatccagt tctttacgtg
    30061 ccgccgcgcg cgccagcgtc tgcttaccgc cacagcagta atccatatca tatttacgaa
    30121 acagagccga agcgcgaggg atagagagcg ccagttcacc taaaggttgg tcgcgataag
    30181 ccatagctga tacctcattc ttaataagat aagatgtatt ttaaatgcat ctttaaggca
    30241 aaagctataa cccttacgta gcagaggctt atttaactca ctgccagcgt gtcaactttc
    30301 tgagacagat tcagttaaag gtttttctgg ttttgctctg accgcaatga ccacgccaat
    30361 cactaacagc gcgataccgc tcagcgtcat tagcggcggc atttgctggc ggagtaaaaa
    30421 ggtgtacagc aaacctgcca gcgtttcgaa aacaatcagc ggcccgagaa tcactgtcgg
    30481 taatcgctgg ctggcgacgt tccagcagag tgcgccaacc catgagcaaa gcacggctat
    30541 cgcaaccatc agactaataa acaccagcgg acgggggcca aaaggtaagg agaagtccgg
    30601 cgtttgtata ttcagccagt aacaggcgac gagatagccg atgagagaaa ccggcagcgt
    30661 gaccagcgcc tgcgccgtcg cccacatcat cggatgtttg tcgggatttt cccgcagcca
    30721 gcgggcgttg cgcagggcat accatgccca gcagaccacg gaaactaacg ctagcacgat
    30781 gccagaggta taacgtgccc agtcaaaatc ggggagtccg tggtttaact cagcaatatt
    30841 cacactcgcc aggccgatgc caatacaaat cagtgccggg gcgagttttc cccacgcgag
    30901 tttgccgtcg cgctggctat aaagcagatt ggcaaagaca ggaatgacca ccggcagggt
    30961 gccgataatc atcgtggaaa caggcgcgcc agtacgttga atggcactgg caaggcagaa
    31021 gtaataaatg aggttgccca tcatagtgag catcaaggcg gtaagccagt cccgacgcgc
    31081 caactgacgc agacgcacgc gtcccagcca ggcaatgggc agcgcaatta accctaacgc
    31141 cagataacgc cccatcgact gcaacatcgc cgggtattcc ggtacgatca acggcccgac
    31201 aaaaataagc ccccacatca accctgctaa cagggcgtac agcacgccgc taatcattac
    31261 tggcatccat tggtctgtcg gaagaaagtt caggaagcag acatgattgc tcccggcatc
    31321 ccgtcattat cagagaggat gcgggaggat gccactttaa ttaactagtt attaacatta
    31381 aaaagatggc ttacgctttc ggctaacgtt gtcgttgggc ggccaatcaa tttgctaagc
    31441 gttttgctgt tatcaaacag accacctttc gatgcgccaa cgtcagaatc cgccagcata
    31501 tccgccagtc cgtcgggcag tccgacgctt ttcagtgccg cggcgaaatc ggcttcgctc
    31561 agattttgat aggtaacgga ttttccgctc tgtttggtca gctctgccgc taactgtgtc
    31621 aacgtccagg cactatcgcc cgccagttcg taaaccttgc cttcgtgacc ggcttcgcta
    31681 atcacgcgtg ccgcagctgc cgcataatct gcccgcgttg ctgaggcgat tttgccatcg
    31741 cccgccgcac cgataaatac gccgtgttcc agtgctgccg gggcgctggc gaggtagttt
    31801 tcggtgtacc agccgttgcg cagcagggtg taaacgatgc cagaatcagc cagcattttc
    31861 tccgtctcga tgtgctcatc ggcgaggccg agcggggagg tatctgcatg tagcaggctg
    31921 gtataagcga taaatttcac gccagccgcc tttgcggcat taataacatt acgatgctgc
    31981 ggggcacgtt gacccacttc gctggaagag atcagcagta gtttttccac tccctgaagt
    32041 gcagatgtca gtgcggcttc atcgccgtag tcagcctgac gcacggtaat gccttgtgct
    32101 gccagggcct gggcttttgc cggattacga acgatagcca ctatttggct ggcaggaacc
    32161 gttttcatca aggattcaat aacatagtga ccaagttggc cagtggcacc agtaatagcg
    32221 atcatgggaa gtctccatcg ttttgtcttg tgtttaaata cgctaacacc taaacttact
    32281 tttagtaagt acgtacaaaa aggtaagtat gaaatgagtc aggttagcct gtcgcaacaa
    32341 ctgaaagagg gtaatctctt tgcggaacag tgcccgtcgc gcgaggtgtt gaaacacgtc
    32401 accagccgtt ggggggtatt gattctggtg gcgctacgcg aaggtactca tcgctttagc
    32461 gacctgcggc gcaaaatcgg tggggtgagt gaaaagatgc ttgcgcagtc gttacaggcg
    32521 ttagaacagg atggttttct taaccgtatc gcgtatccgg tggtgccgcc gcatgtggaa
    32581 tacagcctca cgccgctggg cgagcaggtg agcgaaaagg ttgctgcact ggcggactgg
    32641 attgagttga atttgcccga ggtgttggcg gtgcgggatg aacgtgcggc ataactgccg
    32701 gatgcgctga gcttatctgg tctacggtgc atggcttgta gggcggataa gatgcgccag
    32761 catcgcatcc gccaataaat gccggatgcg gttgatgatt tataggccga aaaagaagtg
    32821 ttttacttac tcaaatccac ctgataaatc gcaaacccga tatcatcggt cgcgacttta
    32881 ttcatcggat attgcccttt ctctttaata aacgctgcgg ctttatctga cggagaagtt
    32941 tcgaaacgga tatccagttt cttatcgcca gctatcggtg ctaaacgcca gttgttatct
    33001 gccgccgggt gaatttcccc cgcacgtttc gactcatcag caatccacgc tgccagaacc
    33061 gagcggttct catccggtga agcaaaagcg atatggctgt cgcccgtccc ggcaaatttg
    33121 ccgccgtaag cgcgatagtt attggtggca acgaggaaca tagcgttcgg atcaatcggc
    33181 ttgccattaa aggtcaggtt cttaatcctt tccgcattgg cattaatcat ctggcactcg
    33241 ccgtcataac gggcgggttg ggtaacatca atctgataat tcacaccatc aataacatca
    33301 aagttataag tgcggaaacc atcccagttg atgagtgact gtggtttcgt gctgttagga
    33361 tcaatctggt taaactgtcc ggcggagcac tccagccact ctttcacctc tttaccgctg
    33421 gctttcacca caatcagcgt attggggtag agataaagat cggcggcatt acggaaggtc
    33481 aactggcctt tttccacctc cacatagctt gccgggtcat ttttgcgacc accaacttta
    33541 aacggtgcgg cagctgaaag cactggcagt tttgccagat ccggatcgct ctgaatgtaa
    33601 tgctcgacat acgctttttg cgcgttgttc accacctgca cggtcggatc gtcctgcacc
    33661 agtgccagat agctatacat attgtcggca gatttaccga ttggcttgct gacgaactgg
    33721 cgtgtggcat cgtgatcggc tttgagtgtt tctaccagct tgctgtcttc cgccgcgagg
    33781 gattttttat tagcgatgtc gtaaatcggt cgtgcttccg ctttcgcctg cgtcacctgc
    33841 catttaccgc tgtcattact gagttgtaag tcgaccacac caagatgatc gccccacatg
    33901 cctggcatta ccgccggaac accattcagc gtgcctttcg cgatatcagc tccttcgata
    33961 tcagcaaaat ctttacttgg gaaaacggcg tgagcatggc caaacataat ggcgtcaaca
    34021 cccggaattt cactgagata ataaactgag ttttccgcca tcactttata cggatcggca
    34081 gacagcccgg aatgcgccag aacgacaacg agatcggcac ctttctcacg catttcaggc
    34141 acgtatttgc gcacagtttc tgttatatcg ttaacggtga ctttgccgga caaattggct
    34201 ttatcccacc ccataatctg cggcggcacg acgccaatat agccaatctt taacgtctgt
    34261 ttttttccgt ctttatcgac cacttcggtg tctttaatta aatacggtgt aaacatgggc
    34321 tgtttggttt ttgcgtcaat gacgttggca tttacataag ggaatttcgc tcctgccaac
    34381 gcatttttca ggtaatccag accgtagtta aactcgtggt tgccaagcgt gccgacggta
    34441 tagtccagcg tatttaatgc cttatagacc gggtgaatat ctccggcttt taatcctttc
    34501 gccgacatgt aatcggccag cggacttccc tggatcaagt cgccgttatc caccagcacg
    34561 ctatttttga cttcattgcg ggcatcgtta atcaggcttg cggtgcgtac cagtccgaac
    34621 ttttccgtgg cggtgtcttt gtaataatcg aaatccatca tgttgctatg cagatcagtg
    34681 gtttccatga tacgcagatc gaccgtcgct gcattcacac tggcggcaat cagcgtggcc
    34741 aggagcgttg cgctaaactt aatcatcagg gacatccttt tattatcggg aatacgaaag
    34801 aaaagggaga ataaacgtct tacttataga acagtgaaga atgccacaat tttacgcttt
    34861 gaaaatgatg acactatcac agttggcgca ttcattaacg atagggtata agtaaaacaa
    34921 taagttaaca ccgctcacag agacgaggtg gagaaatgtt agatcaagta tgccagcttg
    34981 cacggaatgc aggcgatgcc attatgcagg tctacgacgg gacgaaaccg atggacgtcg
    35041 tcagcaaagc ggacaattct ccggtaacgg cagcggatat tgccgcacac accgttatca
    35101 tggacggttt acgtacgctg acaccggata ttccggtcct ttctgaagaa gatcctcccg
    35161 gttgggaagt ccgtcagcac tggcagcgtt actggctggt agacccgctg gatggtacta
    35221 aagagtttat taaacgcaat ggcgaattca ccgttaacat tgcgctcatt gaccatggca
    35281 aaccgatttt aggtgtggtg tatgcgccgg taatgaacgt aatgtacagc gcggcagaag
    35341 gcaaggcctg gaaagaagag tgcggtgtgc gcaagcagat tcaggtccgc gatgcgcgcc
    35401 cgccgctggt ggttatcagc cgttcccatg cagatgcgga gctgaaagag tatctgcaac
    35461 agcttggcga acatcagacc acgtccatcg gctcttcgct gaaattctgc ctggtggcgg
    35521 aaggacaggc gcagctgtac ccgcgcttcg gaccaacgaa tatttgggac accgccgctg
    35581 gacatgctgt ggccgcagct gccggagcgc acgttcacga ctggcagggt aaaccgctgg
    35641 attacactcc gcgcgagtcg ttcctgaatc ctgggttcag agtgtctatt tattaattaa
    35701 tttatgcagc aggtccatca cttgctgcac ctcttcctga gtaagcgccc cgtctttggc
    35761 ccattgcacg cgaccgtctt tatccagcac cactacagcg gaactttttt catccagctg
    35821 ccaggcaccg cgtgcgacgc cattgctatc aacaataaac tgcgaccagg gataaagctt
    35881 tttattactc tccagactgc tgcgcacaaa catgccggaa cccggaattg cgtcgtcggt
    35941 gttaacaatg gtggtggtct ggtaacgatc gtgcggtaac ttcgctgatt taatcgcttc
    36001 aatcagcgtc gcgtttttct cttttgcaga ggtgcgacca gcaatatgtt gcagtactcg
    36061 cacttttccc actaactgcg cgctgttcca ggttttgtag ctaaactgat ctttatcaag
    36121 caccaactcg ccacgatcgg tgatgccaat cggcggcact cgctgaccgg tttcgaactg
    36181 atgtgcggaa gccatcatcg gcaacagcag gcaggtgagt gccagaatct tgcgtagggt
    36241 catggtgttt ccttcttatg atatgcaggt gatccgacca cttgggtctg agttttaatc
    36301 atatgtgcta tttatcgaat ttcccgcaag tgtgatgcca gtttgcggtc aagcgcacaa
    36361 atcatatgaa aaatgaatgc ttatactgaa gaccgcgctt cggtaaaaag ataattctga
    36421 ataattgtaa cctttaggta aaaaaagtta tacgcggtgg aaacattgcc cggatagtct
    36481 atagtcacta agcattaaaa tttgcgcctc ataatagttg ggccgattgt ggcaccgcac
    36541 aggcgtaata ctcagcagga gataacaatg aaaattttcc aacggtacaa cccacttcag
    36601 gtggcgaagt acgtaaagat cctgttccgt ggacggttat acatcaagga cgttggcgct
    36661 tttgaatttg ataagggtaa gattcttatc ccaaaagtga aggataagct gcatttgtca
    36721 gtgatgtccg aagttaaccg tcaggttatg cgtctgcaaa cagagatggc ttaaccaaag
    36781 tgctatgcag taataagacg gctcctgatt caggagccgt tgatgtttct gggggttacg
    36841 cgacgctttc ttctttatct ttagcgtcag gcagttttgg cgaaagggcg gtggccttgc
    36901 tgtcgatccg ggtcaccagc agctggtcga tgcggtagtt atcgatatcc accacttcaa
    36961 atttgtagcc ggcgaatttc accgaatcgg tgcgtttcgg gatcttacgc agcataaaca
    37021 tcataaagcc gccgatggtt tcgtagttgc ccgactgcgg gaactcgtca atatccagca
    37081 cgcgcatgac gtcgtcaatt ggggtgccgc cgtcaatcag ccatgagttc tcatcacggg
    37141 cgacaatctg ctcttccagc ccctgaccga ccagatcgcc catcagcgtg gtcatcacgt
    37201 cgttgagggt gatgatcccc accaccagcg cgtactcgtt catgatcacc gcgaagtctt
    37261 cacctgcggt tttaaaactt tccaacgcct ctgaaagggt taacgtatcc ggcacaatca
    37321 gcgtgttgcg aatttgtacg ccgctgttca gtgccaggct ttggttagcc agcacgcggt
    37381 tcagcaggtc tttagaatcg acataaccga tgatgtggtc aatatcttca ttacagacga
    37441 ggaacttaga gtgcggatgt tccgccacct tattcttcag gctttgctca tcttcgtgga
    37501 gatcaaacca aatcacgttt tcacgcggtg tcattgaaga cggaacggta cgggattcca
    37561 gctcaaagac gttttcaatc agctcgtgtt cctgtttacg taacacgcct gccagcgcac
    37621 cggcttccac taccgcgtag atgtcatcag aagtgatgtc atctttacgt accattggca
    37681 gtttgaaaat acggaagatt atgttcgcca gtccgttgaa gaaccacacc agcggggtgc
    37741 aaacgtacag gcagaagcgc atcgggttga tgatacgcaa agccaccgct tctggcgcaa
    37801 tcataccgat gcgtttcggg gttaaatccg caaacaggat aaacatgcca gtcactaacg
    37861 agaaagagag aataaagctc agttgctcag agagttctgc cgacatatag cgggagaaca
    37921 ggctgtgaaa agctggagaa aatgccgcat caccgacgat accgccgaga atcgccactg
    37981 cgttcagacc gatttggacc acggtaaaga acatgccggg attttcctgc atattcagaa
    38041 cgcgttgggc atttatattg ccttcatcag ccagcagttt aagtttgatt ttgcgtgagg
    38101 cggcaagcga gatctcggac atcgagaaga acgcacttac agcgatcaaa cagagtatga
    38161 ctaaaatact gtttaacata tcttatccga cctttcaggc cagatcctcg gaaggggaag
    38221 ttgattaatt tgtgtgaaat acacattgaa agccgattcg aagaaagtga accggcattt
    38281 tcagtggcta gtatagcgta aggtactgta aagtcgccag agggttaaaa ttcagactgc
    38341 tgataaatgc aatactgcct gatgcgctat gccaatcagg cctacgcagt ccttgcaata
    38401 tattgaattt ggacgatttt gtaggcctgg taaggcgttt acgccgcatc tggcataaat
    38461 aaagcgcact ttatcaacaa tctacatttg tacccgtaac gctatgcttc cggtggcaga
    38521 cagacgccaa ttccgccaat tccacagtaa ccatacgggt ttttatgcag atattgctgg
    38581 tggtcatctt cggcataata aaacggtgtg gcgttagcga tttccgtggt gatgtgacga
    38641 tcatcatcgg cggcaagcat cgccgcctga aaacgttcca gactggcgcg agctgcggca
    38701 tcctgttctg gggtcagcgg ataaatcgct gaacgatact gcgtgccgtg gtcattgccc
    38761 tgacgcattc cctgggcggg atcgtgattc tcccaaaata cctgtagcaa ctgctcatag
    38821 ctgatgacgg aaggatcgta aacaatgcgt accgcttcgg catgacccgt atcaccggag
    38881 cacacttccc gataagtcgg atttggcgta tagcctccgg tatagcctgc ggcggtgctg
    38941 taaacgccgg gtaactgcca gaacagacgc tccacacccc agaaacaacc catcgcaaaa
    39001 atggcaatct ccattccgtc aggtacattg gtcattgagt gaccgttgac cgcatgcagc
    39061 gtggctacgg gcatcggggt gttacgtcca ggcagggcat cggcggggga aaccagatgc
    39121 tttttatcaa ataaactcat ggtgtcgctc tcccgaaaat cggtcattgg ggttaaggtt
    39181 gtaacaagag gcgtatttgc acacaataac caccgtgaat aggtctaaag taaaacataa
    39241 gaaatatttg gggtttagtc tgctttttaa tccatattac tggatttttg ttaagccgtt
    39301 taacggcgtt ccaggggcag gaaaaaagga tattcaggag aaaatgtgcg ctatatccga
    39361 cagttatgct gtgtaagctt actctgctta agcggatctg ccgtcgccgc gaacgtccgt
    39421 ctacaggtcg aggggttatc gggacagctg gaaaagaacg ttcgtgcgca gctttctacg
    39481 attgaaagtg atgaagtgac gccagaccgt cgctttcgcg cacgcgtcga tgatgccatc
    39541 cgtgaaggtc tgaaagcgct gggttattac cagccgacca ttgaatttga tctccgtcca
    39601 ccgccaaaga aagggcggca ggtattgatc gccaaagtca cgccaggtgt gccggtgtta
    39661 attggcggca ccgatgtggt attgcgcggc ggcgcgcgga ccgataaaga ctatttgaaa
    39721 ttgctcgata ctcgcccggc tattggcacg gtgctgaacc agggcgatta tgaaaatttc
    39781 aaaaagtcct taaccagcat tgcgttgcgt aaaggttatt tcgatagcga atttaccaaa
    39841 gcgcagctgg gcattgcgct cggcctgcat aaagccttct gggatattga ttataacagt
    39901 ggcgaacgtt accgctttgg gcatgtgacc tttgaaggat cacaaatccg cgatgaatac
    39961 ctgcaaaatc tggtgccgtt taaagagggc gatgagtacg aatcgaaaga tctggcggaa
    40021 ctgaaccgcc gactttctgc taccggctgg tttaactcgg tggtggtggc tccacaattt
    40081 gataaagcgc gcgaaacgaa agtattaccc ttgacgggcg tggtttcgcc gcgaactgaa
    40141 aacaccatcg aaaccggggt cggttactct acggacgtgg gaccgcgcgt gaaagcgacg
    40201 tggaaaaagc cgtggatgaa ctcttatggt cacagtctga ccaccagtac cagtatttcc
    40261 gcgccggaac agaccctcga cttcagctat aaaatgccgc tgctgaagaa tccactggaa
    40321 caatattatt tggtgcaggg cggttttaag cgcactgacc tgaacgatac cgaatctgac
    40381 tccactacgc tggtggcttc tcgctactgg gatctctcca gcggctggca gcgtgccatt
    40441 aacctgcgct ggagcctcga ccactttacc cagggtgaaa ttaccaacac cacgatgctg
    40501 ttttatcctg gggtgatgat tagccgcacg cgttctcgtg gtggcctgat gccaacctgg
    40561 ggcgactcgc aacgttactc tatcgactac tccaacacgg cctggggctc agatgtcgat
    40621 ttctccgttt tccaggcgca gaacgtctgg atccgcacac tgtacgatcg ccatcgtttt
    40681 gtgacacgcg gcacgctggg ctggattgaa acgggtgatt tcgacaaagt accgccggat
    40741 ctgcgtttct tcgccggggg cgatcgcagt attcgcggct acaaatacaa atctatcgcc
    40801 ccgaaatacg ccaacggtga cctgaaaggg gcctcgaagc tgataaccgg atcgctggaa
    40861 taccagtaca acgtgaccgg aaaatggtgg ggcgcggtgt ttgtcgatag tggcgaagcg
    40921 gtaagcgata ttcgccgcag cgactttaaa accggtaccg gggtcggcgt acgctgggaa
    40981 tcgccggtcg ggccaatcaa actcgatttt gccgtaccgg tcgcggataa agacgagcac
    41041 gggttacagt tttacatcgg tctggggcca gaattatgag tttatggaaa aaaatcagcc
    41101 tcggcgtggt tatcgttatc ttactgttgc tgggatcggt ggcgtttctg gtgggcacca
    41161 ccagcggcct gcatcttgta tttaaagcgg cggatcgctg ggtgccagga ctggatattg
    41221 gcaaggtcac cggcggctgg cgcgatctca ccttgtctga cgttcgttat gagcagccag
    41281 gcgtggcggt aaaagcgggt aatctgcatc tggcggtcgg tcttgagtgc ctgtggaaca
    41341 gtagcgtttg tattaatgac ctggcgctga aagacattca ggtcaacatc gacagtaaaa
    41401 aaatgccccc ttctgaacag gttgaagaag aggaagatag cggtccgctg gatctctcca
    41461 cgccgtatcc catcaccctg acacgggtgg cgctggataa cgtcaacatc aagattgatg
    41521 acaccacggt atcggtgatg gacttcacct ccggcctgaa ctggcaggag aaaaccctga
    41581 ccctgaaacc gacgtcgctg aaaggcctgt tgattgccct gccgaaagtg gcggaagtgg
    41641 cgcaggaaga agtggtcgaa ccgaaaattg aaaatccgca gccggatgaa aagccgctcg
    41701 gcgaaacgtt gaaagatctc ttttctcgtc cggtattgcc ggaaatgacc gacgtgcatt
    41761 tgccgcttaa cctgaacatt gaagagttta agggcgagca gctgcgcgtg acgggcgaca
    41821 cggacatcac cgtgaacacc atgctgctga aagtaagcag catcgacggt aatactaaac
    41881 tggacgccct ggatatcgat tccaatcaag ggatcgtcaa cgccagcggc acggcacagc
    41941 tgtcagacaa ctggccggtg gatatcaccc tcaacagcac actaaacgtg gagccgttga
    42001 aaggtgaaaa agtgaagctg aaagtgggcg gcgcgctgcg cgaacagctg gagattggcg
    42061 ttaacctttc cggtccggtg gatatggatt tacgcgccca gacgcgactg gcggaagctg
    42121 gattgccgct caacgtggaa gtgaacagca aacagcttta ctggccgttc actggtgaga
    42181 agcagtatca ggcggatgat ctgaaactga aacttaccgg caaaatgacc gattacacgc
    42241 tctctatgcg tacggcagtg aagggacagg agatcccgcc cgcgactatt accctcgacg
    42301 cgaaaggtaa tgaacagcag gtcaatctcg acaaactcac cgttgcggcg ctggaaggca
    42361 aaactgaact caaggcgttg ctcgactggc aacaggccat tagttggcgc ggtgagctaa
    42421 cgcttaatgg cattaacacc gccaaagagt tcccggagtg gccgtcgaaa ctcaatggct
    42481 tgattaaaac ccgcggtagc ctgtacggcg gcacctggca gatggaggtg ccagaactga
    42541 agctgaccgg taacgtcaaa cagaacaaag tgaacgttga cggcacgctg aaaggcaaca
    42601 gttatatgca gtggatgatc ccagggcttc atctggaact ggggccaaac agtgccgaag
    42661 tgaaaggcga gctgggggta aaagatctca atcttgatgc caccatcaac gcgccggggc
    42721 tggataacgc gctgccgggg cttggcggta cagcgaaagg gctggtgaaa gtacgcggca
    42781 cggtggaagc gccacaacta ctggcagata tcaccgcgcg cggcctgcgc tggcaggaac
    42841 tttccgtggc gcaggttcgc gtggaaggcg acattaaatc caccgatcag atcgccggga
    42901 aactcgacgt acgcgttgag caaatttcgc agccggatgt aaatatcaac ctcgtcaccc
    42961 tgaatgccaa aggcagcgaa aagcagcacg agctacagtt gcggattcag ggcgagcctg
    43021 tctccgggca gcttaatctg gcaggaagtt ttgatcgcaa agaagaacgc tggaagggaa
    43081 ttcttagcaa tacccgcttc cagacgccgg tcggcccgtg gtcgctgacc cgcgatattg
    43141 cgctggatta ccgcaataag gagcaaaaaa tcagcatcgg gccacactgt tggcttaacc
    43201 cgaatgcgga gctgtgtgtg ccgcaaacta tcgatgcggg ggccgaaggg cgtgcggtgg
    43261 tgaatctcaa ccgcttcgac ctcgccatgc tgaaaccgtt tatgccgcaa accactcagg
    43321 ccagcggtat cttcacgggt aaagcggatg ttgcctggga caccacgaaa gaggggctgc
    43381 cgcagggcag tatcaccctt tcggggcgta acgtgcaggt aacgcaaacc gtcaacgatg
    43441 cggcgctgcc ggtggcgttt cagacactga atctgacggc ggaattgcgt aacaaccgtg
    43501 ccgaattggg ctggaccatc cgcctgacca ataacggcca gtttgatgga caggtgcagg
    43561 tgaccgatcc gcaaggccgc cgtaatcttg gtggcaacgt caatatccgt aacttcaacc
    43621 ttgcgatgat aaaccccatc tttacccgtg gggaaaaagc agcggggatg gtgagtgcca
    43681 acttgcgtct gggtggtgat gtgcaaagcc cgcagttgtt tggtcagctt caggttacgg
    43741 gtgtggatat cgacggcaac tttatgccgt ttgatatgca gccgagccag cttgcggtca
    43801 actttaacgg tatgcgctcg acgcttgccg gtacagtacg gacccagcag ggtgaaatct
    43861 acctgaacgg tgatgccgac tggagccaaa ttgaaaactg gcgggcacga gtaacggcga
    43921 aaggcagtaa agtgcggatc accgtgccgc cgatggtacg aatggatgta tcgccagatg
    43981 ttgtattcga ggctacacca aacctgttta ccctcgatgg tcgcgtggat gtcccgtggg
    44041 cgcgcatcgt ggtgcacgat ctgccggaaa gcgcagtagg cgtctccagc gatgtggtga
    44101 tgcttaacga taacctgcaa ccggaagagc cgaaaacggc gtcgattccg attaacagta
    44161 acctgattgt ccacgttggc aacaatgtgc gcattgacgc ctttggcctg aaagcgcggc
    44221 tgacgggcga tcttaacgtc gtacaggaca aacaagggct gggcctgaac gggcagatca
    44281 acatccctga agggcgcttc catgcctatg gtcaggatct gattgtgcgt aagggtgagt
    44341 tactgttctc tggtccgccg gatcaaccgt atcttaatat tgaagctatt cgtaacccgg
    44401 atgctacaga agacgacgta atcgccggag ttcgcgtcac tggtctggcg gacgaaccga
    44461 aagcggagat cttctctgac ccggcgatgt cgcaacaagc tgcattgtct tatttgctac
    44521 gtggacaagg gctggagagc gatcagagcg acagtgcggc aatgacctcg atgctgattg
    44581 gtctgggggt tgcgcaaagt ggccagattg tgggtaaaat cggcgagacg tttggcgtaa
    44641 gcaatttagc gctcgacacc cagggagtag gcgactcctc ccaggtagta gtcagcggct
    44701 atgtattgcc aggtctgcaa gtgaaatacg gcgtgggtat atttgactct atagcaacac
    44761 tcacgttacg ttatcgcctg atgcctaagc tatatctgga agccgtgtct ggtgtagacc
    44821 aggcactgga tttgctctat cagttcgagt tttagcaatg cgaatatttg tctacggcag
    44881 tttacgccac aaacaaggca acagtcactg gatgaccaat gcccagttac tgggcgattt
    44941 cagtatcgat aactaccagt tgtatagcct gggccactat ccaggcgcag ttccggggaa
    45001 cggaacagta cacggtgaag tttatcgtat tgacaacgcc acgctggccg aacttgatgc
    45061 cttgcgcacc aggggcggtg aatacgcgcg ccagttgatt cagacgccgt acgggagtgc
    45121 atggatgtac gtttatcaac gacccgtcga tggattaaag ctaattgaaa gcggcgactg
    45181 gttagacagg gataagtaac catatgcata cgccaccttc gggtggcgtt gttttttgcg
    45241 agacgactcg cattctgttt tgtaattccc tcaccctttg cttttctctc cgagccgctt
    45301 tccatatctg ttaacgcata aaaaactctg ctggcgttca caaatgcgca ggggtaaaac
    45361 gtttcctgta gcaccgtgag ttatactttg tataacttaa ggaggtgcag atgcgtatta
    45421 ccataaaaag atgggggaac agtgcaggta tggtcattcc caatatcgta atgaaagaac
    45481 ttaacttaca gccggggcag agcgtggagg cgcaagtgag caacaatcaa ctgattctga
    45541 cacccatctc caggcgctac tcgcttgatg aactgctggc acagtgtgac atgaacgccg
    45601 cggaacttag cgagcaggat gtctggggta aatccacccc tgcgggtgac gaaatatggt
    45661 aaagaaaagt gaatttgaac ggggagacat tgtgctggtt ggctttgatc cagcaagcgg
    45721 ccatgaacag caaggtgctg gtcgacctgc gcttgtgctc tccgttcaag cctttaatca
    45781 actgggaatg acgctggtgg cccccattac gcagggcgga aattttgccc gttatgccgg
    45841 atttagcgtt cctttacatt gcgaagaagg cgatgtgcac ggcgtggtgc tggtgaatca
    45901 ggtgcggatg atggatctac gcgcccggct ggcaaagcgt attggtctgg ctgcggatga
    45961 ggtggtggaa gaggcgttat tacgcttgca ggcggtggtg gaataaggtg tgtttattta
    46021 tcgcgggcat aaaaaaaccc ttactaaccg aagcccggtg ttcagggtta ttgcgccaga
    46081 agaacttatt tattctttgc gcgctcgaag gaggcaacga tttcagcttt agcggcttct
    46141 gcgttttccc aaccttcaac tttcacccac ttgccttttt cgaggtcttt gtagtgctcg
    46201 aagaagtgag cgatctgcgc tttcagcagc tctggcagat cgttaacgtc tttaatgtga
    46261 tcgtattctt tgctcagctt ggtgtgcgga acagcaatca gtttcgcatc ttcaccggct
    46321 tcgtcggtca ttttcagaac gccaaccgga cggcaacgga tcacagaacc cggctgcagc
    46381 gggtacggag tcgggaccag tacgtcaacc gggtcaccgt ccagagacag ggtgtggttg
    46441 atgtaaccgt agttgcacgg atagaacatc gcggtggaca tgaagcggtc aacgaacagt
    46501 gcgccgctct ctttgtcgat ttcgtatttg atcggatctg cgttagccgg gatctcaata
    46561 accacgtaga tgtcttccgg cagatcttta cccgcaggga cgttgagtaa gctcatgtct
    46621 gtttccttta aaaatatgtg gcaaacaagt gccgagtatt atagccaact cgcgccgaat
    46681 gtcttcgctt gttttcgtct tcattttcct ttttcgccag ttttaagacg gtatgcagag
    46741 caggaaaatc cataaccgta gccgcatttt tcatagtgag atgaaagcga ttacaaactt
    46801 gtgattaacg ttttatttac ttttttgaag tgtgatgtaa cgcaatccgt tacataacga
    46861 attgtctata gttttttcgc gaacatcttt tcaccaataa taattacccc gacgaggaca
    46921 accctatgtg gaaacgctta cttgtagtct ctgcagtctc ggcagccatg tcgtctatgg
    46981 cgttggccgc tccgttaacc gtaggatttt cgcaggtcgg atcggaatca ggctggcgtg
    47041 ccgctgaaac caatgtggcg aaaagtgagg ccgaaaaacg cggaattacg ttgaaaattg
    47101 ccgatggtca acaaaagcag gaaaaccaga ttaaagcggt acgttccttc gttgcacaag
    47161 gggtggatgc gatctttatc gctccagtgg tagcgacggg ttgggagccg gtattaaaag
    47221 aggcgaaaga tgccgaaatc ccggtcttct tgcttgaccg ttccatcgat gtgaaagaca
    47281 aatctctcta tatgaccact gtcaccgccg acaacatcct cgaaggcaag ttgattggtg
    47341 actggctggt aaaagaagtg aatggcaaac catgcaacgt ggtggagctg cagggcaccg
    47401 ttggggccag cgtcgccatt gaccgtaaga aaggctttgc cgaagccatt aagaatgcgc
    47461 caaatatcaa aattatccgc tcgcagtcag gtgacttcac ccgcagtaaa ggcaaagaag
    47521 tcatggagag ctttatcaaa gcggaaaaca acggcaaaaa catctgcatg gtttacgccc
    47581 ataacgatga catggtgatt ggtgcaattc aggcaattaa agaagcgggc ctgaagccgg
    47641 gtaaagatat cctcacgggt tccattgatg gcgtaccgga tatctacaaa gcgatgattg
    47701 atggcgaagc gaacgccagt gttgaactga cgccgaatat ggcaggtccc gccttcgacg
    47761 cgctggagaa atacaaaaaa gacggcacca tgcctgaaaa gctaacgctg accaaatcca
    47821 ccctttatct gcctgatacc gcaaaagaag agttagagaa gaagaaaaat atggggtatt
    47881 gagggtatca atgcctgatg ccgattcgta ggccggataa ggcgctcgcg ccgcatccgg
    47941 cggtggtgcg ctgaagcctg atgcgacgct taccgcgtct tatcatgcct acgagttcgg
    48001 tgctctgtgt aggccggata aggcgttcac gccgcatccg gcagtggtgc aacaccggac
    48061 ttaacaccgt gcacaatctg tcccctcgcc ccttcgggga gagggttagg gtgaggggga
    48121 caaacggcgc agctcttaac atcaggcacg atctattccc agacttttag agcgtggcta
    48181 agggggaaac catgacgacc gaccaacacc aggaaatcct ccgcaccgaa ggattaagta
    48241 aatttttccc cggcgtcaaa gcgttagaca acgttgattt cagcctgcgc cgtggcgaaa
    48301 tcatggcgct gctcggtgaa aacggggcgg gaaaatcgac gctaatcaaa gcattaaccg
    48361 gtgtatacca cgccgatcgc ggcaccatct ggctggaagg ccaggctatc tcaccgaaaa
    48421 ataccgccca tgcacaacaa ctcggcatcg gaaccgttta tcaggaagtc aacctgctac
    48481 ccaatatgtc ggtcgctgat aatctattca taggtcgcga acccaaacgt ttcggctttc
    48541 tacgccgcaa agagatggaa aagcgtgcca ccgaactgat gacatcttac ggtttctccc
    48601 tcgacgtgcg tgaaccgctt aaccgttttt cggtggcgat gcagcaaatc gtcgccattt
    48661 gccgggctat cgatctctcc gccaaagtgc tgatcctcga tgaacccacc gccagcctcg
    48721 acacccagga agtagagtta ctgtttggcc tgatgcgtca gttgcgcgat cgcggcgtca
    48781 gcctgatctt tgtcactcac tttctcgatc aggtctatca ggtcagcgat cggatcaccg
    48841 tcttacgcaa cggcagtttc gtaggctgtc gggaaacgcg cgagctaccg cagatcgaac
    48901 tggtaaaaat gatgctgggg cgcgagctgg atacccacgc gctacagcgt gccgggcgaa
    48961 cattgttgag cgacaaaccc gttgccgcgt tcaaaaatta cggcaaaaaa ggaacgatcg
    49021 caccgtttga tctcgaagta cgccccggcg agatcgtcgg cctggcaggc ttgctgggat
    49081 ccggacgtac cgaaaccgcc gaagtgatct tcggtatcaa acctgctgac agcggcacgg
    49141 cgttgatcaa aggcaaacag caaaacctgc gatcgccaca tcaggcttcg gtactgggca
    49201 tcggcttctg cccggaagac aggaaaaccg atggcatcat cgccgccgcc tcggtacggg
    49261 aaaatatcat cctcgctctc caggcccagc gcggctggct acgacccatt tcccgcaaag
    49321 agcagcaaga gattgccgaa cgctttatcc gccagcttgg cattcgcaca ccttcaactg
    49381 aacaaccgat tgaatttctc tctggcggca atcagcaaaa agtgttgctt tcacgttggc
    49441 tactgacccg accgcaattt ctgatcctcg acgagccaac ccgcggcatt gatgttggtg
    49501 cccacgccga gatcatccgc ctgattgaaa cgctgtgtgc tgacggtctg gcgctgttgg
    49561 tgatctcctc cgaactggaa gagctggtgg gctatgccga tcgggtgatt atcatgcgcg
    49621 atcgcaaaca ggtggcggag atcccgctgg cagagctttc cgttccggcg atcatgaacg
    49681 ccattgcggc gtaaggagaa cagtgtgatg cctcaatctc tcccggacac tacgccgccg
    49741 aaaaggcgct ttcgctggcc aacgggaatg ccgcagctgg cggcgctatt gctggtgctg
    49801 ctggtcgata gcctggtagc cccgcatttc tggcaggtgg tgcttcagga tgggcgtttg
    49861 ttcggtagcc ctatcgacat tcttaaccgt gcagctcccg ttgcgttact ggcgattggt
    49921 atgacgctgg tgatcgccac cggtgggatt gatctctccg taggggcggt gatggctatc
    49981 gccggagcca caacggctgc gatgacggtc gcgggattca gcctgccgat tgttttgtta
    50041 agcgccctgg gcaccggcat cctggcggga ttgtggaacg gcatactggt agcgatcctc
    50101 aaaattcagc cgtttgtcgc caccctgatc ctgatggtcg ccgggcgcgg cgtggcgcaa
    50161 ctgatcaccg ccgggcagat cgtcacgttt aactcgccgg atctctcatg gtttggcagc
    50221 ggatcgctgt tgttcctgcc aacgccggtc attatcgcgg tgctgacgct tctcctgttc
    50281 tggctgttga cccgcaaaac ggcgctgggg atgtttatcg aagccgttgg tatcaacatt
    50341 cgggcggcaa aaaatgccgg ggtaaacacg cggatcatcg tcatgctcac ctacgtgttg
    50401 agcgggctgt gtgcggcgat tgcgggcatt atcgtggcgg cggatattcg cggtgccgat
    50461 gccaataacg ccgggttatg gctggagctg gacgccattc ttgcggtggt gattggcggc
    50521 ggatcgctga tgggcgggcg ctttaacctg ctgctctcag tcgtgggggc gctgattatt
    50581 caggggatga acaccggaat tttactttct ggctttccgc cggagatgaa ccaggtcgta
    50641 aaagcggtgg tggtgctttg tgtgctgatt gttcagtcgc aacgctttat cagtctgatt
    50701 aaaggagtac gtagccatga taaaacgtaa cttgccgctg atgatcacca tcggcgtctt
    50761 tgtgctgggc tatctctact gcctgaccca gtttcccggt tttgcttcca caagagtgat
    50821 ctgcaatatc ctgaccgata acgcctttct tgggataatc gccgttggca tgacctttgt
    50881 gatcctctct ggcggtatcg atctctccgt cggttcggtg atcgccttta ctggcgtgtt
    50941 tctggcaaaa gtgattggcg atttcggcct ctcgccgctg ctggcgtttc cgctagtgct
    51001 ggtgatgggc tgtgcctttg gcgcatttat ggggcttttg atcgacgccc tgaagatccc
    51061 ggcatttatc attaccctgg caggaatgtt ctttttgcgc ggcgtcagct atctcgtttc
    51121 agaagagtcg atcccgataa accatcccat ttatgacacg ctctcaagcc ttgcgtggaa
    51181 aatccctggc ggcggtcgct taagtgcgat gggactgctg atgctggcgg tggtggttat
    51241 cggcatattc ctcgcgcatc gtacccgttt tggtaatcag gtatacgcca ttggcggcaa
    51301 cgcaacgtcg gcaaacctga tggggatttc tacccgcagc accactattc gcatttatat
    51361 gctctccacc ggactggcaa cgctggcggg gattgtcttc tcgatttata cccaggccgg
    51421 atatgcgctg gcgggcgtag gtgtggaact ggatgctatc gcctcagtgg taattggcgg
    51481 tacgcttttg agcggtggcg ttggaacggt attagggacg ctttttggcg tggcgattca
    51541 ggggctaatt cagacttata tcaactttga cggtacgctg agttcctggt ggacgaaaat
    51601 cgccatcggc attttgctgt tcatttttat tgccttacag cgtggattaa cggtgctgtg
    51661 ggagaatcgt cagagttcgc cagtgacaag agtcaatatt gcgcagcgat aaaaacgcct
    51721 ctccgtgtgg agaggcgcag gagattacgc gtccgggaat tcacggataa agcgttcgac
    51781 atcttcaacc atatggtcgt tgccgacaaa gaacgaacgg cgctggtgca gagtttccgg
    51841 gatgatatcc agaatacgct ctttgccatc gctcgcttta ccgcccgctt gttccgccag
    51901 gaatgccatc gggttgcact catacagcaa acgcagtttg ccgtccgggt ggctggcggt
    51961 gcttgggtag agataaatac cgcctttcag caggttacgg tggaaatccg cgaccagtga
    52021 accgatataa cgtgaggtat acgggcggtt ggtggattta tcttcttcct ggcagaattt
    52081 aatgtacttc ttcaccccgt tcggaaactt aatgtagttt ccttcgttga tggagtaggt
    52141 tttgcctttc tccgggaagc gcatccgttc ctggcacagg cagaaaacgc ccagcgaagg
    52201 atcgtaagta aaggcgtgaa caccgcatcc ggtggtgtaa accagcatgg tagaggagcc
    52261 gtataccacg taacctgccg caacctgttt gttaccaggc tggaggaaat cttcttccgt
    52321 taccggcgtg ccaacaggcg taacgcggcg gtagatggag aaaatggtac cgacagagac
    52381 gttaacatcg atgttggacg agccatccag ggggtccatc agcaccacgt attttgcgtg
    52441 ttcacagcct tcaaagacga caatctcatc ttcttcttca gaggcaatgc ccgcaacgat
    52501 atcgcgtgct ttcagtgcgg ctttcagttt ttcattagcg aacaagtcga gtttctgctg
    52561 aacctcgccc tgcacgttct cagcaccgct ggcacccagg atatcaacca gtcctgcttt
    52621 gttgatatcg cgatggataa tcttggcgcc cagttttatt gccgacagca aagcagtgag
    52681 ctcaccggta gcatgagaaa actcgtgctg cttttcgaca ataaattcac ctaacgtttt
    52741 cataaaactt tccctgcaat gtttatggag taaagcgacc gcaacaatct taacaaataa
    52801 tctcaatgtt gcgctcaggt gaatcgcgcc agcaaattgc ggattatcct gaaatgcgtt
    52861 tctcacttgc ccgacatatg cgtaaaatga gcggcagatt aaaaaaggat agtgacgtat
    52921 gcgcattcat attttaggaa tttgtggcac atttatgggc ggtctggcga tgctggcgcg
    52981 ccagttaggc catgaagtaa cgggttcgga cgccaatgtg tatccgccga tgagcacctt
    53041 acttgagaag caaggcattg agctgattca gggttacgat gccagccagc tcgatccgca
    53101 gccggatctg gtgattattg gcaacgccat gacccgtgga aatccgtgtg tggaagcggt
    53161 actggaaaaa aacatccctt atatgtcagg tccacagtgg ctgcacgatt ttgtgctgcg
    53221 cgaccgctgg gtgctggccg ttgccggtac acacggcaaa actaccaccg cgggaatggc
    53281 gacctggatt ctggaacagt gcggttacaa accgggattt gtgatcggcg gtgtgccggg
    53341 gaactttgag gtttcggcgc gtctgggcga aagcgacttc tttgttatcg aagcggatga
    53401 gtatgactgc gccttcttcg acaaacgctc taaatttgtc cattactgcc cgcgtacgct
    53461 gatcctcaac aaccttgagt tcgatcacgc cgatatcttt gacgacctga aagcgatcca
    53521 gaaacagttc caccatctgg tgcgtatcgt tccggggcag ggccgtatta tctggccgga
    53581 aaatgacatc aacctgaaac agaccatggc gatgggctgc tggagcgagc aggagctggt
    53641 gggtgagcag ggtcactggc aggcgaaaaa gctgaccact gacgcttctg aatgggaagt
    53701 cttgctggat ggcgaaaaag tgggcgaagt gaaatggtcg ctggtgggcg aacataatat
    53761 gcacaatggc ctgatggcga ttgcggcggc tcgccatgtt ggtgtagcgc ctgcagatgc
    53821 cgctaacgcg ctgggttcgt ttattaacgc tcgtcgccgt ctggagttgc gtggtgaagc
    53881 gaatggcgtg acggtatatg acgattttgc ccatcacccg acggcaattc tggcaacgct
    53941 tgcggcgctg cgtggcaaag ttggtggtac ggcgcgcatt attgctgtgc tggagccgcg
    54001 ctcgaatacc atgaaaatgg ggatctgcaa agacgatctg gcaccttcat taggtcgtgc
    54061 cgatgaagtc ttcctgctgc aaccggcgca tattccgtgg caggtggcag aagtggcaga
    54121 agcctgcgtt cagcctgcac actggagtgg cgatgtggat acgctggcag atatggtggt
    54181 gaaaaccgct cagcctggcg accatattct ggtcatgagc aacggcggtt ttggtgggat
    54241 ccatcagaaa ctgctggatg gtctggcgaa gaaggcggaa gctgcgcagt aattcggcct
    54301 cagcctgaga tagcattgcc ggataaggcg tttacgccgc atccggcatt tgagcatagt
    54361 gcctgatgcg acgcttgatg cgtcttatcc ggcctacagg tgcaccgcat ccggcaaacc
    54421 atactcacat caacaacgaa aattaccctt cattctccgc cagttcgcgc agatactgga
    54481 aaatctggcg tgcggatttc ggcggcttat tcccttcttt ctctttcttc gcgttacgga
    54541 tcagagtacg cagctgctga cgatcggcat cgggccacag attcaatact tcagcgatgg
    54601 cgtcatcacc ttgatcgatc agacgatcgc gcaggttttc cagtttatgg aacagcacca
    54661 cctgctggtt gtgacggttt ttcagcttat ccagcgcctg acgaataggc tctacgtcgc
    54721 gctggcgcag catcttaccg atgagctgca actggcggcg gcgaccttcc atcttaatac
    54781 gctgggcgag ctcaatagcc gcgcgcagat ccgcatctaa cgggatctta tccagcgcgt
    54841 ttttccccag atcaacaatt tccgcgccaa ggcgttttag ctcctcggca tcacgtttaa
    54901 tttcactttt actgacccag ataatttcat cgtcttcgtc ttcgatgtca tcaccgggaa
    54961 cgtcgtcgag ccagtcttcg ggctgcttag tcatctcagg ctccttaaaa aaagaggcta
    55021 atgttaccag ttaagatgcg cactgaaaaa cggttctctg ttagacttca gagaaactct
    55081 ctacattatg gcacttgcaa tgaaagtaat ctctcaagtt gaagcgcagc gcaagattct
    55141 ggaagaagca gtttccactg cgctggagtt ggcctcaggc aaatcggacg gtgcggaagt
    55201 tgccgtcagc aagaccaccg gcattagcgt aagcacgcgt tatggtgaag tggagaatgt
    55261 tgaattcaat agcgatggcg cgctggggat cactgtttat caccagaacc gcaaaggtag
    55321 cgcatcatcc accgatttaa gcccgcaggc cattgcccgc accgtacagg cggcgctgga
    55381 tattgcccgt tatacctcgc cagatccctg tgccggcgtg gcagacaaag agctgctggc
    55441 ctttgacgca ccagatctcg acttgttcca ccctgcggaa gtttccccgg atgaagccat
    55501 tgaactggcg gcccgcgcag aacaggcggc attgcaggcg gacaaacgca ttaccaatac
    55561 cgaaggcggc agttttaaca gccactacgg tgtcaaagtt tttggcaaca gccacggcat
    55621 gttgcagggt tactgctcta cgcgtcattc actctccagc tgtgtaattg ccgaagaaaa
    55681 tggcgatatg gagcgtgatt acgcctacac cattggtcgt gcgatgagcg atctgcaaac
    55741 gccagagtgg gttggggccg actgtgctcg ccgtacttta tcgcgcctgt caccgcgtaa
    55801 actctccacc atgaaagcgc cagtcatttt tgccaatgaa gtggcaaccg ggctttttgg
    55861 ccatctggtg ggggcgatag cgggtggctc ggtttaccgt aaatctacct ttctgctgga
    55921 ttcgctgggt aaacaaattc tgccggactg gctgaccatt gaagagcatc cgcatctgct
    55981 gaaagggctg gcgtcgacgc cattcgacag cgaaggtgtg cgcaccgagc gtcgcgatat
    56041 tattaaagat ggcatcctga ctcagtggct gctgaccagc tactcggcgc ggaaactggg
    56101 gctgaaaagc accggacatg cgggcggtat tcacaactgg cggattgccg gacaagggct
    56161 aagcttcgaa caaatgctca aagagatggg caccgggctg gtggtgacgg agctgatggg
    56221 ccagggcgtg agtgccatta ccggtgatta ttcccgtggt gcagcgggct tctgggtaga
    56281 gaatggcgaa attcagtatc ccgttagtga aatcaccatc gcaggtaatt taaaagatat
    56341 gtggcgcaat attgtcaccg tcggtaacga tattgaaaca cgcagtaata tacagtgtgg
    56401 ttctgtgctg ttgccggaga tgaaaatcgc cgggcagtaa aaaactggcg cgacctgccg
    56461 cgctataaac taaaaaatta cacaaaatca ttcgcactgc atcgagacgg aaagtgaatg
    56521 agggccgggg agcgtactcg cagtacgtga cccgggcgaa tgagcgtagc cgtcgatgag
    56581 gcagtgtgaa ggatgaagtg taaataaaaa aggaagtgag caatgcgtaa aagcctgtta
    56641 gctattcttg cagtctcctc gttggtattc agttctgcgt cgtttgccgc tgatcttgaa
    56701 gacaatatgg aaaccctcaa cgacaattta aaagtgatcg aaaaagcgga taacgcggcg
    56761 caagtcaaag acgcgttaac gatgatgcgc gccgcagcgc tggatgcgca aaaagcaacg
    56821 ccgccgaagc tcgaagataa atcaccggac agcccggaaa tgaaagattt ccgccacggt
    56881 ttcgacattc tggtcggtca gattgacgac gcgctgaagc tggcaaatga aggtaaagta
    56941 aaagaagcgc aggccgccgc agagcaactg aaaacgaccc gcaacgccta tcaccagaag
    57001 tatcgttaat tcctcatttc cctgttgcct gcactcaggt aacagggaac ttatcatcgc
    57061 aaatgatgca ccacctgatt gctgctgccg cgccagatca gggaagggtc ttttaaatcc
    57121 tgcacaaact tgccgtcgac cagcacgtta atcaaatcaa caacctgcat ttgcgtagcg
    57181 ttgagttcgt cgagtttata gcctgtccac acccagatgt ctttacccgg acactcggcg
    57241 cggatgcgtt gtaccagttt cagaatatcc ggcacgtttt gcggatgcag cggatcgccg
    57301 ccggagaggg aaataccctg gcgtttgata cgagtgtcat tcagatcgtt aatgatctgg
    57361 tcttccattg ctttggtaaa tggctgacca gaatttaccc gccatgtgct tttgttatag
    57421 caaccggggc attcatgaac acacccggag acaaacaggg tgcagcgagt gccggggccg
    57481 ttgacgatgt cgacaggata gtactgatga taattcatta tttcgcctgg cttatcagtc
    57541 cgcgtgtagg ctgcatccgg taatcaatgc ctgatgcgac gctgtcgcgt cttatcaggc
    57601 ctggagccgt tgccaaatgt aggccggata aggcgtttac gccgcatccg gcatttgctt
    57661 agcgcagagt gaagattaac ctatctgccc attccccaaa tgtttaacgc ggcgcttaac
    57721 ttcttcctgc ttaccagcgt taaacggacg tgcatccggg ctacctaaat atccgcacac
    57781 gcggcgagtt accgacacac gggaggcgtc atggttacca catttcgggc aagtgaagcc
    57841 tttgctggtg cactcgaact caccggtaaa gccacactcg tagcactcat caatcggtgt
    57901 attggtgccg taatacggta catgctgata gctgtaatcc cagacatctt ccagcgcctt
    57961 caggttgtgc tgaatgtttg gatactcgcc gtagcaaatg aaaccaccgt tcgccagcgg
    58021 cgggtaaggc gcttcaaagt cgatcttgtc gtacgggttc accttcttct ccacatcgag
    58081 gtggaaactg ttggtgtagt aacctttatc ggtcacgccc ggcaccacgc caaactcagc
    58141 agtatcgaga cggcagaagc ggtcgcacag gttttcactc ggcgtgctgt agagactgaa
    58201 accataaccc gtttcttctt tccactgatc cactgcctga cgcagacgtt caacaatggc
    58261 gatacctttc gcgcgaagct gctcgttgtc gtaaacatgc tcgccgccga acagcgcgtt
    58321 aatggtttcg tggatgccga tgtaacccag cgaaatagac gcacgaccgt ttttgaagat
    58381 ttcagaaaca tcatcgtcag cattcagacg tacgccacaa gcaccttcca tatagaggat
    58441 cggggcgacg cgcgctttca cgccttcgag acgagcgata cgggtcatca gcgccttacg
    58501 tgccagcacc agacgttcat ccagcagctt ccagaaggtg gcttcatcgc cttttgcttc
    58561 cagagcaata cgcggcaggt tcaggctgat cacgccgagg ttgttacgac catcgtggat
    58621 ctgctcgccg ttttcatttt cccacacgcc gaggaagctg cggcagccca tcggggtttt
    58681 aaacgaaccg gtgactttca ctacctgatc gtagttcagg atatccggat acatgcgctt
    58741 gcttgcgcac tccagcgcca gctgtttgat gtcgtagttc ggatcgcctt ttttatggtt
    58801 cagaccatcg cgaatcgcaa acaccagttt cgggaacacc gcagttttac ggtttttacc
    58861 gaggcctgcg atacggttac gcaggatcga ttcctgaatc aggcgcgatt cccagctggt
    58921 gcccagacca aaaccaaagg ttacaaacgg cgtctgaccg ttggcggtgt gcagggtgtt
    58981 tacttcgtac tccagcgact ggaaggcatc gtagcactct ttgatggtac gagagtttgc
    59041 gtagccttcg gcgtccggga tgttccactc ttctgcggtc ttgcgatgtt tgttgtagct
    59101 ggcggtaaca aacggcgcca gcacttcatc gatacggtta atggtggtgc cgccataaat
    59161 atggctggcg acctgagcaa taatctgcgc agttaccgcg gttgccgtag agatcgactt
    59221 cggcggttca atctcggcgt tccccatctt aaagccctgg gtcagcatgc ctttcaggtc
    59281 gatcaacatg cagttgaaca tcgggaaaaa cggcgagtaa tcgagatcgt gatagtgaat
    59341 atcgccacgc tcatgtgcct tcaccacgtc acgcggcagc aggtgctggc gtgcatagtg
    59401 tttagccacg atcccggcca gcaggtcgcg ctgggttgga atcaccttgc tgtctttgtt
    59461 ggcgttttcg ttgagtaacg aggagttggt ctgctcgacc agaccacgga tctcctggtt
    59521 caggcgaccg cgtttttcac gttcaatgtc gcgatcgtga cggtactcga tgtaagcacg
    59581 agccagttgt ttgtatggac ctgacatcag ctgattttca actgcggtct ggatctcatt
    59641 gatatccacc tggttgcggc cctgcatctg ctcgctgaca accgcggcaa cagtggcgca
    59701 ataatcggca tcatcgactt ccgctgcttt agctgcacgc agaatcgctt ctttgatgcg
    59761 ctctgattta aacggcactt tgcagccgtc tcgtttcatc acatgcggtg tcatgatcgc
    59821 tccatatttt taagaacagg ttatccacag aaattgggaa aggcgttttc cggttttttc
    59881 gtttcgtttt ccggtgcttt ccgcaatcgc catccgctta tccacaacgc cgcacctttt
    59941 tcgtggggag ctgtagtagc aattatagtc gattaataca acatattggg tttggacgca
    60001 ttttaaagtc tatatatagt gctttgcatc aaggatgttt taactttttt tgatgtagct
    60061 caaagtaaaa agcagagcgt acggatgacg ggcgctacag cgttatgtaa attttttaat
    60121 gaatttgctg gttgaaaaat caacaaaaac aacatgctga cagacaaaac cccggaatga
    60181 ccggcattac cggggcttag ggaagattta cttctgtaac caccagacag cctcaaaagg
    60241 ccgtaaattc atggcacagg gttgtggtga ggcttcttcg tagttatgca tcacaagctg
    60301 ccagttgccg cgcatttgcc ctggctgcca gggttggatc tcacggctaa ggttggcaat
    60361 gaccagcaag gtttgcccct tccattcacg gcgatagcac cacaatacag ggctgtttgg
    60421 cagcagatcc tggtaattgc cccatgtcag gatggcttct tgcttacgca gtgcgattaa
    60481 cttttggtag gtgtaaaaca ccgaggactc atcggccagc gcggcttcta cgttgatttt
    60541 ttgatagtta tcgcccaggc caatccacgg ttcgccagcc gtaaacccgg cattatcgcc
    60601 gttgctccat tgcatgggcg tgcggctgtt gtcacgggat ttactggcga ggattgccaa
    60661 taactcgtcg gcatcacgac catcgttgcg cagctcggca aacatattta ggctctccac
    60721 gtcgcgatag tcagtaatgc gcgtgaaatg cgggttggtc atgccaatct cttcgccctg
    60781 gtagatatac ggcgttccct gcatgccatg cagcaccatc gccagcattt ttgccgcagg
    60841 cacgcggtat tcaccttcat caccaaagcg agaaacaatg cgcggctgat cgtggttaca
    60901 ccagaacaag gcattccatg ccacgttgtg cattccttgt tgccagtggc ggaacaatgt
    60961 tttcaacgcc acaaagtcag gtttagccag cgtccatttt tcaccaccgg gataatcgac
    61021 cttcaggtga tgaaaattaa aggtcatcga caattcactg cctgtcagtg ccgcgtatcg
    61081 ctggcaatgc tcaaggctgg tggaggacat ttcacctacg gtcattaacc cgcgtggcgt
    61141 aaacacatcg cggttcatct cgtgcaaaaa ctcgtgtgct cgtggcccgt cggtgtagaa
    61201 gcgacgcccg tcgccatcca ggtcttcagg gaaacgcggg tctttggaga tcagattcac
    61261 cacatccagg cgcaacccgt cgaccccacg atcggcccag aattcacaga cttttttcag
    61321 ctctgcgcgt accgctggat tctcccagtt gagatccgcc tgttctggtg caaagagatg
    61381 caaatagtac tgttcgcttt ccgcatgcca gcgccacgca ctaccgccaa attttgaacg
    61441 ccagttgttc ggtggcgttt ctggttcgcc atcgcgccag atataaaact ggcggtaagg
    61501 gctttctttg ttcagcgcct cgcgaaacca ggcatgttgg gtagaggtat ggttaaacac
    61561 catatcgaga atgatacgaa tcccgcgcga ttttgcctgc gtcaccagtt cgtcaaaatc
    61621 gtccagcgtg ccgtaggtgg gatcaatcgc cgtatagttc gctacatcgt aaccgttatc
    61681 gacctgggga gagacataaa agggggttag ccagatggca tcaacgccca gtttatgcag
    61741 atagtccagg cgttggataa cgccacgtaa atcgccggtg ccgctacccg tggtgtcctg
    61801 aaaactcttt ggataaatct ggtagataac gccgttttgc caccagtggg gaagattagt
    61861 cattacgtta ttcctgcaaa tgcgaggggg cgcaattgcg ccccgaagaa aattaaacaa
    61921 tgtccagcgt gcccaggcgg tatttccgct gatagataaa cgaggtgagt acaatcggga
    61981 tgatgatggc gatagccatt gccagcgcaa atacctgcca gtagctcggt tgaatcgaga
    62041 gaattcccgg caggccccct acgccgatac cattcgccat aacgccgttc aggccgcata
    62101 gtaatcctgc cagaccagaa ccaatcatcg cgcacagcat cgggaagcga tatttcaggt
    62161 tgatgccgta cattgcaggc tcagtgaccc caagccaggc ggagatagcg gcaggcacgg
    62221 agatctcgcg ttcattgtgc ttgcggctgg aaatgataat gcctatcacg gcggagccct
    62281 gagcgatatt cgacagcgca atcagcggcc acactggcgt gccacccatg ctttgaatca
    62341 tctgcaaatc aatagcaagc gtggtctggt gtacaccggt gatcaccagc ggggcgtaca
    62401 ggaagccaaa caatgctgcg ccaatcggag caaagctgcc ggtcatcagg tgacgtaccg
    62461 caaaggcaac gccatcgcca atcatgcgac caaacggacc aatcagcgca tgggcgagga
    62521 acaccgcgag gatcagcgaa cagacgggca ccaccaccag atagaggtaa tccggcacga
    62581 tgcgtttaag gcgagtttca ataacgccca gcgccagccc ggctaacagt gccgggatca
    62641 cctgcgcctg atagcctact ttggcgatgc tgaacatgcc aaagtcccac acttccggca
    62701 gctgctgccc gagcagataa gcgttcatca gctggggaga aaccagtgtc acgccaagca
    62761 cgataccaag gatcggcgtg ccgcccattt ttttcaccgc tgaccagcaa ataccgaccg
    62821 gcaggtagaa gaagatcgct tcaccgatca accacagaaa atcgtagatc gtttgcaggg
    62881 aagggtacat ttgcgccagc gtctgaccgt tgctcatggg caaatcgccg atcacattgc
    62941 gaaaaccgag gatcaaaccg ccgctaatca acgcgggcag tagcgggaag aagatctccg
    63001 cgaaatgaga gatcaattgc tcatgccatt tcatattctg ccgggcggct ttttttacct
    63061 gctctttatc aacctgcgcc tgtccggttg acgcaatcag tgcttgatag taatcaccca
    63121 cattggtgcc aatcaccacc tgaaattgcc cggcattggt gaaacagcct ttcaccatgg
    63181 ggagttgctc aatttctttc ggtctggcat tggccggttg gttgaggaca aagcgcaggc
    63241 gagtaataca gtggctcacc gtcgcaatat taccgcgccc gccgaccagt tcaatcaacc
    63301 gatcgatatc cgtttggttt attttgctca tcataaagcc ccatggcaga tgacattttt
    63361 ggttggctgc agaatatatt gcgcggaaaa atttaaaaac gggaacgttc ccgaaacgca
    63421 gcgaagatca caatttatcg ttcaggaaac gatcaggaca gggtggcggg gatgatgatt
    63481 tgttgcggtt cgctgcgccc ggttacctgt gcgatcaatt ggcaggccgc ctggcgtcca
    63541 gcttcggcgt aaccaggatc tacggtgacg atctccggat ggaggaattt cattaatggc
    63601 gtattaccga cgctcgccag ttgcaaggtg tcgatgcgtt gctcttgcag gtatttactt
    63661 gcgccaagtg ccagcgtgtc ggttgcgcac agtaaggcgg tagtttcagg cgtaatcact
    63721 tttgctacgt tctcatagcc ttgcttcata gcaaggcctg gcagagcggc aacgggatgc
    63781 agtttatgcg ctttgcagaa cgccaggtag gcttcgtgac gtcgcttccc ggtcgtcacg
    63841 tcactatgcg gcacgccgag ataactgata ttacgatgcc cctggtcata cagccgttgc
    63901 atcaggatct tgattgcccc ttcgtcgtca taacagaccg aagcaaaacc ttttgcgtca
    63961 cgcgccagca gaaccagcga tgactgccag tgggctaaca tttcttctgt tatgccagta
    64021 aaaccgaaca gcactacgcc gtcgatatta cgccgtttca gcacccccaa atgttcggca
    64081 actaattgcg gggaaaactg actttccatc atgattgggt cgtaaccttg ttcatagaac
    64141 gctggcagca tggtttgaac ggcgagattt tctgacaacg aatccaggcg ggtaacaatg
    64201 atggcgacca ctttatcgct ttgcccacgc atagcgcgcg cagagcggga aggggaaaat
    64261 ccatgctgat tcatcactgc ttcaacacgc tcgcgggtgc gctggctcac gccgctttcg
    64321 ttattcagca cccgggaaac tgtagatttc cccacgccgc ttaagcgtgc gatgtctttg
    64381 atggtcagcc gattttgcat cctgttgtcc tgtaacgtgt tgtttaatta tttgagccta
    64441 actttacccg tgcattcagc aatgggtaaa gtctggttta tcgttggttt agttgccagc
    64501 aggtattata tcgccataga tgctacgaat attattggat tctccttatt atttgcggcg
    64561 ctttattcac ttaccggagg ttatatggaa cctgatccca cgcctctccc tcgacggaga
    64621 ttaaaacttt tccggtaagc ccgtcttttc acggcgttac cggatgcgta aggccgtgac
    64681 gttttaacgt ccctgctcag ctttattacc ttcaggtaag gcttcgccac gcctgaagac
    64741 atttctgtac tgtttcagac agtgcggagg gactccttat gtttaaagaa atctttaccc
    64801 ggctcattcg ccatttacct tcccgtctgg ttcatcgtga tccattgcct ggcgcgcagc
    64861 agacagtgaa tacggtggtc ccgccgtcct taagtgcgca ttgcctgaaa atggcggtga
    64921 tgcccgaaga agaattatgg aaaacgttcg acacccatcc ggaagggtta aatcaggcgg
    64981 aagtggaatc tgcccgcgaa caacatggtg aaaataaatt acccgcacaa caaccgtcgc
    65041 cgtggtgggt acatttatgg gtctgctatc gcaacccatt taatatttta ctcaccattc
    65101 tcggcgctat ttcttacgcc acggaagatt tatttgccgc gggcgttatt gcgctaatgg
    65161 tcgctatttc aacgttactg aactttattc aggaagcacg ttccactaaa gcggcagatg
    65221 ccctgaaagc gatggtcagc aataccgcga cggtgctgcg cgtaattaac gacaaaggcg
    65281 aaaatggctg gctggagatc ccgatcgacc agctggtgcc cggcgatatt ataaaactgg
    65341 cggcgggaga tatgatcccg gcagatttac gtatcttgca ggcgcgggat ctgttcgtcg
    65401 ctcaggcgtc gttaaccggt gagtctctgc ccgtggaaaa agccgctacc actcgccagc
    65461 cggagcacag caatccgctg gagtgcgaca cgctgtgttt tatgggcacc accgtggtga
    65521 gcggcacggc acaagcaatg gtgattgcta caggtgccaa tacctggttt ggtcaactgg
    65581 cggggcgtgt tagtgagcag gaaagcgagc cgaatgcctt tcagcagggg atcagccgcg
    65641 tcagtatgct gctgattcgc tttatgctgg tgatggctcc ggtggtgttg cttatcaacg
    65701 gttacaccaa aggcgactgg tgggaagcgg cactgtttgc gctttcggtc gcggtaggct
    65761 taacgccgga aatgttgccg atgattgtta cctcgacgct ggcgcgcggc gcagtaaagc
    65821 tgtcgaaaca gaaagtgatc gtcaaacatc tggatgctat tcagaacttt ggcgcaatgg
    65881 atattctgtg cactgataaa actggcaccc tgacacagga caaaattgtg ctggagaatc
    65941 ataccgatat ctccggtaaa accagcgaac gcgtgctgca tagcgcgtgg ttgaacagtc
    66001 attatcagac cggacttaaa aacctgctcg atacagcggt gctcgaaggt acggatgaag
    66061 agtcagcgcg ctcgctggcc agtcgttggc aaaaaattga tgagattccg tttgatttcg
    66121 agcgtcgccg gatgtcggtg gtagtggcag aaaataccga gcaccatcag ctggtttgca
    66181 aaggtgcatt gcaggaaatc ctcaatgtgt gttcgcaggt gcgtcacaat ggcgagattg
    66241 taccgctcga tgacatcatg ctgcgtaaga ttaagcgggt tactgatacg cttaatcgtc
    66301 aggggctgcg cgtggttgcg gtggcgacga aatacctgcc agcgcgtgaa ggagattacc
    66361 agcgggcgga tgaatccgac ctgatcctcg aaggatatat tgctttcctt gatccgccaa
    66421 aagagacaac cgctccggca ctgaaggcat taaaagcgag tggaatcacc gtaaaaatcc
    66481 ttactggcga cagtgagtta gttgcagcga aagtgtgcca tgaagtgggg ctggacgcgg
    66541 gagaggtggt cattggtagt gatattgaaa cgctatctga cgacgaactg gcaaatctcg
    66601 cacagcgcac cacgctgttt gcccgcctga cgccgatgca taaagaacgc attgtgacct
    66661 tactgaagcg cgaagggcat gtggttggct ttatgggcga tggtattaat gatgcgcccg
    66721 ccttacgcgc tgcggatatc ggcatttctg tggacggcgc ggtagatatt gcccgtgaag
    66781 cggctgatat catcctgctg gaaaaaagcc tgatggtgct ggaagagggg gttattgaag
    66841 gacgtcgcac tttcgccaac atgctgaaat acatcaaaat gacggcgagc tccaacttcg
    66901 gtaatgtgtt cagcgtgctg gtggcgagtg ctttcttgcc cttcctgccg atgttgccgt
    66961 tacacttgct tattcagaac ctgctgtatg atgtgtcaca ggtggcgatc ccgtttgata
    67021 acgtcgacga cgagcaaatt caaaagccgc agcgttggaa tccggcggat ctggggcgct
    67081 ttatgatctt cttcggaccg atcagctcga tcttcgatat tctgacgttt tgcctgatgt
    67141 ggtgggtatt ccatgccaac acgccggaaa cgcaaacgct gttccagtcg ggatggtttg
    67201 tggtgggctt actgtcgcaa acgctgattg tgcatatgat ccgcacccgc cgtgtgccgt
    67261 ttattcagag ctgtgcatcg tggccgttaa tgatcatgac cgtgatcgtg atgattgtcg
    67321 ggatcgcatt gccgttttca ccgctggcca gttatctgca actgcaggcg ctgccgttaa
    67381 gctatttccc gtggctggtt gcgattctgg cagggtatat gacattaacc cagttggtga
    67441 aaggatttta tagccgtcgt tacggctggc aataaagaat caactgggca tggtggcctg
    67501 gattcgaatt gcagacaaag tgagttttgt tcatgccgga tgcggcgtaa atgccttatc
    67561 cggccaacaa attcaataaa ttgcaggaat cacgtaggcc tgataagcgt agcgcatcag
    67621 gcgattttgt gtttgtcatc agacttaatc cgggcatgat agcccggatt tccatcaaga
    67681 ttagcgacga acagcgatcg cttcgatctc aattttcacg tctttcggca gacgggcaac
    67741 ttcaacgcaa gaacgtgccg ggaaggtggc attgtgttcg gtgaagaagg cttcgtaggt
    67801 agcattaacg gttgcgaagt cgttcagatc tttcacaaac acggtggttt taacgatgtc
    67861 gcccactttc aggcctgcgg cttcgacgat cgcttttacg ttatccagcg actgacgtgc
    67921 ctgtgcagcg acgtctgccg gtacttcgcc cgttttcgga tttaccggga tctggccgga
    67981 ggtgatgatc atattaccca gatcaacgcc ctgtacgtaa ggaccgatag ctgccggtgc
    68041 attttccgtc gcgatagttt tgctcatgat ttctccttta ttacagcggt aaaaagtctg
    68101 gctcattata gggagccaga cttttattac caaccgcaat taattggcca gcaccacatt
    68161 atgggaaaac tctttttcac agtatttgca tttgagcgcg atgtcattgg cgcgttttcg
    68221 cacggcaaag ctggaagaaa ccggttcggc atggctgata cagttgctgt tcgggcagac
    68281 cagcacattg tcgatgcgct ctggcagact ggggcgcgat ttacccacca cttcatagtt
    68341 gtcgatacgg ttaaccgtgg cttgcggcgc atacaatgcc agttgatcta cctgatcttc
    68401 actcaaaaag gtattttcga ttttgatcag atctttgcgg cccatctcgc cagaaggcaa
    68461 gttcagaccg atggtgatgc gctgatccgt ttcggtcagc ttgaacagac tcaacagctt
    68521 aaaaccgatc tgggcgggga tatggtcaat taccgtgccg cgtttaatag cttcaacctg
    68581 caatttatta tcgtgtgtca tctctatttc cccttacagt accagatcgc gattcagaac
    68641 cagtgccagt aacgcctggc gagcgaaaat cccgttgcct gcctgctgga agtaccaggc
    68701 gtgtggcgtt ttatcaacat ccgtcgcaat ctcatcaaca cgcggcagcg gatgcagcac
    68761 tttcatattg gctttggcgt tgtggagatc gctggcgcga agaacaaact gcgctttcac
    68821 gttggcgtac tcggacgggt ccagacgctc tttttgcacg cgggtcatgt acaggatgtc
    68881 tacttccgcc atcacttctt caatagagct gtgcagactc catgcgatcc ctttttcatc
    68941 gagcatatcc agaatgtatt gcggcattgc cagcgcgtcc ggcgcgatga agtaaaaacg
    69001 gttgccgtcg aacttcgcta acgcctgagt cagggagtga acggtgcggc catatttcag
    69061 gtcaccaacc attgcgacgt ggagattgtc cagacgcccc tgggtttcct gaatagtgaa
    69121 taagtccagc aaggtttgcg tcggatgttg gttggagcca tcaccggcat tcagtaccgg
    69181 tacattgccg gaaaactcgg tggccaggcg cgccgcacct tcctgcggat gacgcatcac
    69241 tatcgcatcg acgtaagtgc tgataaccga aatggtatcg gccagcgttt cgcccttttt
    69301 acccagtgat gtattggcgc tgtcggagaa gcccaccacg ctggcaccca ggcggtgcat
    69361 agatgtttcg aaagagaggc gggtacgggt agaggcttcg aagaaacagc tggcaatgac
    69421 tttgtgcttc aacagctctg gttgcgggtt tgctttcagt ttcgccgctg tcgccagcac
    69481 cagattaagg tcatcgcgac taaggtcgtt tatggaaatg atatgtttct gatatagcgg
    69541 attagccatc ttttatctcc tgacgcctgg gcaaaaaaaa gcccctcgat tgaggggctg
    69601 ggaatgggtg atcaacggga agaaaaacgg caggccagcg tcttttttca gacgcggtaa
    69661 gacaaaatgt cgaacacact gaaccataca tcctcccggc aaattgtccg gcattatact
    69721 catcgtcaga agcggtgcaa gcatttgatg caatattttg tcagcgcaaa cggtttattt
    69781 gaattaaaag tcaaggtata tgcattttta tttgatgtga ttctgcaggg gaactgtcct
    69841 tcggtatcaa taattgcaga cattccctgc tttttccttt ttcactttca cgcaatcaga
    69901 tatgcatttt attccattca tccgacttat agggcggagt gtgaaaacga acggctaaca
    69961 ctattgctta ctgctcaggg atgcgcgcta tcactttaat ttcaaaatca aagcctgcca
    70021 gccatgtaac acctaccgcc gtccagtttg gataaggtgg ggcgctaaat atttcatttt
    70081 tcaccgtcat gatgtcttcg aattgttttt ctggatcggt atgaaagctc gtaacatcaa
    70141 tgatatcgtc aaaagtgcat cccgcagcag ccagggtcgc atgcaaatta tcaaatgcca
    70201 gtctgacttg ttgctgaaaa tcgggttctg gtgttccgtc ctctcgactt cctacttgcc
    70261 cggaaacaaa cagcaaatcg ccggagcgaa tagccgcaga ataacgatgt tcagcataca
    70321 gtgaatgtcg gccagcaggg aaaacagcgg ttctttctac catttggtta tcctcaagat
    70381 ttacgacatg aacagaagat ttatatttac caggagccgc ttttagcgga cgacgtgagt
    70441 aaacaaaacc cagacatcat gggaaatggc tgggcttaat tgagcgtagt cggttatgcg
    70501 ccaaacgcgc catcaatggt atgcatcgcg ccggtaacaa agctggcttc tggccctgct
    70561 aaccatgcga ccataccagc gacctcttcc ggttgcccat gtcttttgat agccataaaa
    70621 ccatgcaaca tatcgcgcat tggcccgttg gcgggattag cgtcggtatc aattggccct
    70681 ggctggacga cgttgatggt gatcccacgc ggtccaaaat cacgggccag cccgcgcgcc
    70741 atgccttgca gggcagattt gctggcggca taagcagcca tgcctgcaac aggcatacga
    70801 tcgccattca cggagccgat gattaagatg cgcccgcctt cgggcatctg ccgggcggct
    70861 tcaacggagg catggtaagg agcatgaata ttgattttga aaaggcgatc aatatcgtcg
    70921 gcatttaatt ccagggcatc gccaaagacg ccaatacctg catttaccac gaggatatcc
    70981 aatgcgccgc tcttacgaac gacatcaata acagcgtctc tgtcagcact atctgtgaat
    71041 actgctgttg ctccagtctc ttgtgccagg cgttcagcgg catctttcga ccccgcatag
    71101 gtgaatcgta cattggcccc atcggtgacg aaacgacgta cgatagcggc accgattcca
    71161 cggctaccac cgaggatgag aactgtctta cctgtaaaag cgcccataag gactccttga
    71221 tttattatgt aacatgcatt acaaaactgt tttaactttc tgtcaacaag ttttgtaatg
    71281 gtcactaaaa aacaatctcg cgttccaggt cgtcccagac gtttcgctcc tgagcaggca
    71341 gtctctgcgg caaaagtgct ttttcaccaa aaaggtttcg atgctgtcag tgttgctgaa
    71401 gttactgatt atcttggtat taaccccccg agcctctacg cggcttttgg cagtaaggct
    71461 gggttattta gccgtgtact caatgaatat gtcggtacgg aagctattcc gcttgtcgat
    71521 attcttcgtg atgatcgtcc agtaggggag tgcctggctg aggtattaaa agaagcggcg
    71581 cgcagatata gccaaaacgg cggctgcgct ggctgtatgg ttcttgaagg cattcatagt
    71641 catgatccac aagcgcgtga tattgccgtt caatattatc acgccgcaga aacggccatt
    71701 tatgactata tcgccaggcg gcatccacaa agcgcacaat gtgtgactga ttttatgagt
    71761 accgtgatgt cagggctttc tgcgaaggca cgagaggggc actccctcga acaactctgt
    71821 gcaacagctg cactggcggg gggagcgata aaaactattc tcaaggagtg atgcgggcct
    71881 tgacccgaaa ggcgggaacg cgcctgccga taagttgtga taagacaata attcacgcgt
    71941 taaggctagc ggaattgatc atcttttcgt ataacgatag aaatgaaacg ttgttttaat
    72001 taaggagtgg aaaagatgat catcggaaat attcataatc ttcagccgtg gctaccccag
    72061 gagttacgcc aggcgattga gcatatcaaa gcacacgtta cggtagaaac gccaaagggc
    72121 aagcacgata tcgaaggcaa tcgtctgttt tatcttatct cggaagatat gaccgagccg
    72181 tacgaagctc gccgtgcgga gtatcatgcc cgctatctcg acattcagat tgtgttaaaa
    72241 ggtcaggaag gcatgacctt cagcacgcaa cctgcaggca cgccggatac cgactggtta
    72301 gctgataaag acatcgcatt tttgccggaa ggcgttgatg agaaaacagt tatccttaat
    72361 gaaggtgatt ttgttgtgtt ttatccgggg gaagtgcata aaccgctgtg cgcagtgggc
    72421 gcgccagccc aggttcgcaa agcagtagtg aagatgctga tggcgtgatg acttttcgcc
    72481 gtaaataact ccaggtttac ggcgagtttg tgaaaagagc gttttttgat atttttttgt
    72541 gagtagaatt tgtaatgctt agacgttctt attaactcaa ggagtccgtc atgagcaaaa
    72601 tatcaggttg gaatttttct caaaacatta catcagccga caattgtaaa caaaaaaatg
    72661 aagacttaga tacctggtat gtgggaatga atgattttgc ccgaattgcc ggagggcaga
    72721 atagcagaag caatattctt tctcccagag catttttgga gtttttggct aagatgttta
    72781 ccctgggtta tgtggatttt agcaaacgct ccaacgaagc gggtagaaat atgatggctc
    72841 atattaagtc ctcatcttat atcaaaaata atgatggtag tgagataatg aagtttgtta
    72901 tgaataatcc tgaaggggag cgagcggatt tatctaaggt ggaaatagaa attacacttg
    72961 cctcagcttt taataatggg attcgtgaag gacatacagt cattatattt ccacaacctg
    73021 atggttcgac taatcgttat gaagggaagt cctttgaaag aaaagatgag agttcattac
    73081 acctgattac taacaagatt ctggcgtgtt accaacgcga agctaacaag gaaatagctc
    73141 gtctattaaa tatttcgcag gagttaaata attctcagga cttgaataac tcgcaggtga
    73201 gttgtaaaga ttcagttgat tctacgatta cggatttatt agaaaaacca ttgaataatg
    73261 cattattagc aataaggaaa gaacatctgc tattaatgcc ttatgtatgt aatgaatcga
    73321 tttcatactt gcttggtgaa aaagggatac tgaaagaaat aga
//
LOCUS       DAPHDW010000004        69590 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.004, whole genome shotgun sequence.
ACCESSION   DAPHDW010000004 DAPHDW010000000
VERSION     DAPHDW010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 69590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 69590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..69590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.004"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1363
                     /gene="htrE"
                     /locus_tag="RH057_000224"
     CDS             <1..1363
                     /gene="htrE"
                     /locus_tag="RH057_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDV3126648.1"
                     /translation="LGLNTSVGAFSFDVTHSNVRIPDDKTYQGQSYRVSWNKLFEETS
                     TSLNIAAYRYSTQNYLGLNDALTLIDEVKHPEQDLEPKSMRNYSRMKNQVTVSINQPL
                     KFEKKDYGSFYLSGSWSDYWASGQNRSNYSIGYSNSASWGSYSVSAQRSWNEDGDTDD
                     SVYLSFTIPIEKLLGTEQRTSGFQSIDTQMSSDFKGNNQLNVSSSGYSDNARLSYSVN
                     TGYTMNKASKDLSYVGGYASYESPWGTLAGSVSANSDNSRQVSLSTDGGFVLHSGGLT
                     FSNDSFSDSDTLAVVQAPGAQGARINYGNSTIDRWGYGVTSALSPYHENRIALDINDL
                     ENDVELKSTSAVAVPRQGSVVFADFETVQGQSAIMNITRSDGKNIPFAADIYDEQGNV
                     IGNVGQGGQAFVRGIEQQGNISIKWLEESKPVSCLAHYQQSSEAEKIAQSIILNGIRC
                     QIQ"
     gene            1380..1949
                     /locus_tag="RH057_000225"
     CDS             1380..1949
                     /locus_tag="RH057_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414680.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3126649.1"
                     /translation="MIKITPHKIAILMGILLSPSVFATDINVEFTATVKATTCNITLT
                     GNNVTNDGNNNYTLRIPKMGLDKIANKTTESQADFKLVASGCSSGISWIDTTLTGNAS
                     SSSPKLIIPQSGDSSSTTSNIGMGFKKRTTDDATFLKPNSAEKIRWSTDEMQPDKGLE
                     MTVALRETDAGQGVPGNFRALATFNFIYQ"
     gene            1964..2566
                     /gene="yadL"
                     /locus_tag="RH057_000226"
     CDS             1964..2566
                     /gene="yadL"
                     /locus_tag="RH057_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000143198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like protein YadL"
                     /protein_id="HDV3126650.1"
                     /translation="MTFKNLRYGLSSSVVLAASLFSVPSYAATDSIGLTVITTVEIGT
                     CTATLVNDSDQDISVVDFGDVYISEINAKTKVKTFKLKFKDCAGIPNKKAQIKLTKRA
                     TCEGTANDGAGFANGSTATDKASAVAVEVWSTETPATGSATQFSCVTPASQEVTISNA
                     ANAVVYYPMSARLVVEKNKTVSNVTAGKFSAPATFTVTYN"
     gene            2593..3189
                     /locus_tag="RH057_000227"
     CDS             2593..3189
                     /locus_tag="RH057_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3126651.1"
                     /translation="MHPTQRKLMKRIILFLSLQFPITYPAIAGQDIDLVANVKNSTCK
                     SGISNQGNIDLGVVGVGYFSDNVTPESYQPGGKEFTVTVSDCALQGTGDVLNQLHIDF
                     RALSGVMAAGSRQIFANEVATGAKNVGVVLFSIQDPTNIFNVISSAGNSRSVYPVMTS
                     ALHNSSWKFYARMQKIDPALDVISGQVMSHILVDVYYE"
     gene            3242..4516
                     /locus_tag="RH057_000228"
     CDS             3242..4516
                     /locus_tag="RH057_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial-like adhesin"
                     /protein_id="HDV3126652.1"
                     /translation="MKKFFRHFLFLILCLSCYTASAGTDDNVGYIVGNSYGVGPSDQK
                     WRETGPNGDATVIFRYATSTNNLVFYKPTQLGPTGVKLQWSQLDTASGGGFLYCNRSD
                     STSGSAMRIENAMVDSGKMYGSHKLFNTSVPGLYYTLLISNMWSAYGTVTNVSSPGIY
                     IGDSAEQYFSWYNPSEDVLYWSCNNANSTRKYWAVGGIYQTLTIEFYTDTNFDPTVTQ
                     QIKLSSSSNYLYSFKAYGAGQGINEHSYFIKIDFDLLNVKLTNPTCFTAMLSGTSVTG
                     STVKMGEYSAEQIRNGATPVPFDISLQNCVRVTNIETKLVSTKVGTENGQLLGNTLTG
                     NDAAKGVGVLIEGLATSKNPLMTLKPNDSNSVYKDYDPRGKDDTTGGVYPDQDTGITY
                     PLHFQATLQQDGTIPIEAGEFKATSTFQVTYP"
     gene            4629..5423
                     /gene="panB"
                     /locus_tag="RH057_000229"
     CDS             4629..5423
                     /gene="panB"
                     /locus_tag="RH057_000229"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HDV3126653.1"
                     /translation="MKPTTIASLQKCKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVEDIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGDEAG
                     DQLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            5435..6286
                     /gene="panC"
                     /locus_tag="RH057_000230"
     CDS             5435..6286
                     /gene="panC"
                     /locus_tag="RH057_000230"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HDV3126654.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHDGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(6360..7262)
                     /gene="rpnC"
                     /locus_tag="RH057_000231"
     CDS             complement(6360..7262)
                     /gene="rpnC"
                     /locus_tag="RH057_000231"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HDV3126655.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEESLKGHSTDVLYSVQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEADHDKLPLVVPILFYQGEATPYPLSMCWFDIFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGESMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMTNLSLAEIDKVINLI"
     gene            7536..7916
                     /gene="panD"
                     /locus_tag="RH057_000232"
     CDS             7536..7916
                     /gene="panD"
                     /locus_tag="RH057_000232"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HDV3126656.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(7920..9149)
                     /locus_tag="RH057_000233"
     CDS             complement(7920..9149)
                     /locus_tag="RH057_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HDV3126657.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAIWAQIGDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSAGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNKINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATAFVVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLETMSRLMSNQPQG"
     gene            complement(9213..9653)
                     /locus_tag="RH057_000234"
     CDS             complement(9213..9653)
                     /locus_tag="RH057_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3126658.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRERIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(9758..10528)
                     /locus_tag="RH057_000235"
     CDS             complement(9758..10528)
                     /locus_tag="RH057_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3126659.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(10525..11451)
                     /locus_tag="RH057_000236"
     CDS             complement(10525..11451)
                     /locus_tag="RH057_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3126660.1"
                     /translation="MTIALELQQLKKTYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            11560..12222
                     /gene="can"
                     /locus_tag="RH057_000237"
     CDS             11560..12222
                     /gene="can"
                     /locus_tag="RH057_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HDV3126661.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(12263..12799)
                     /gene="hpt"
                     /locus_tag="RH057_000238"
     CDS             complement(12263..12799)
                     /gene="hpt"
                     /locus_tag="RH057_000238"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HDV3126662.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            13005..15395
                     /gene="gcd"
                     /locus_tag="RH057_000239"
     CDS             13005..15395
                     /gene="gcd"
                     /locus_tag="RH057_000239"
                     /EC_number="1.1.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
                     /protein_id="HDV3126663.1"
                     /translation="MAINNTGSRRLLVTLTALFAALCGLYLLIGGGWLVAIGGSWYYP
                     IAGLVMLGVAWMLWRSKRAALWLYAALLLGTMIWGVWEVGFDFWALTPRSDILVFFGI
                     WLILPFVWRRLVIPASGAVAALVVALLISGGILTWAGFNDPQEINGTLSANATPAEAI
                     SPVADQDWPAYGRNQEGQRFSPLKQINADNVHNLKEAWVFRTGDVKQPNDPGEITNEV
                     TPIKVGDTLYLCTAHQRLFALDAASGKEKWHYDPELKTNESFQHVTCRGVSYHEAKAE
                     TASPEVMADCPRRIILPVNDGRLIAINAENGKLCETFANKGVLNLQSNMPDTKPGLYE
                     PTSPPIITDKTIVMAGSVTDNFSTRETSGVIRGFDVNTGELLWAFDPGAKDPNAIPSD
                     EHTFTFNSPNSWAPAAYDAKLDLVYLPMGVTTPDIWGGNRTPEQERYASSILALNATT
                     GKLAWSYQTVHHDLWDMDLPAQPTLADITVNGQKVPVIYAPAKTGNIFVLDRRNGELV
                     VPAPEKPVPQGAAKGDYVTPTQPFSELSFRPKKDLSGADMWGATMFDQLVCRVMFHQM
                     RYEGIFTPPSEQGTLVFPGNLGMFEWGGISVDPNREVAIANPMALPFVSKLIPRGPGN
                     PMEQPKDAKGTGTESGIQPQYGVPYGVTLNPFLSPFGLPCKQPAWGYISALDLKTNEV
                     VWKKRIGTPQDSMPFPMPVPVPFNMGMPMLGGPISTAGNVLFIAATADNYLRAYNMSN
                     GEKLWQGRLPAGGQATPMTYEVNGKQYVVISAGGHGSFGTKMGDYIVAYALPDDVK"
     gene            complement(15442..16992)
                     /gene="cueO"
                     /locus_tag="RH057_000240"
     CDS             complement(15442..16992)
                     /gene="cueO"
                     /locus_tag="RH057_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HDV3126664.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLTEETTLHW
                     HGLEVPGEVDGGPQGIILPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEVLKLMLPKQWGIDDVPVIVQDKKFNADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            17158..17505
                     /locus_tag="RH057_000241"
     CDS             17158..17505
                     /locus_tag="RH057_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HDV3126665.1"
                     /translation="MKTFFRTVLFGSLMAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLPNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            17611..18477
                     /gene="speE"
                     /locus_tag="RH057_000242"
     CDS             17611..18477
                     /gene="speE"
                     /locus_tag="RH057_000242"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HDV3126666.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFKLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            18493..19287
                     /gene="speD"
                     /locus_tag="RH057_000243"
     CDS             18493..19287
                     /gene="speD"
                     /locus_tag="RH057_000243"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HDV3126667.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(19325..19687)
                     /locus_tag="RH057_000244"
     CDS             complement(19325..19687)
                     /locus_tag="RH057_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HDV3126668.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDGEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(19863..22460)
                     /gene="acnB"
                     /locus_tag="RH057_000245"
     CDS             complement(19863..22460)
                     /gene="acnB"
                     /locus_tag="RH057_000245"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HDV3126669.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVDVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLNSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLADIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            22815..24572
                     /locus_tag="RH057_000246"
     CDS             22815..24572
                     /locus_tag="RH057_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
                     /protein_id="HDV3126670.1"
                     /translation="MKMTLPFKPHVLALICSAGLCAASTGLYIKSRTVEAPVEPQSTQ
                     QTAPDITAVTLPATVSAPPVTPAVVKSAFSTAQIDQWVAPVALYPDSLLSQVLMASTY
                     PANVAQAVQWSHDNPLKQGDAAIQAVSDQPWDASVKSLVAFPQLMALMGENPQWVQNL
                     GDAFLAQPQDVMDSVQRLRQLAQQTGSLKSSTEQKVITTTKKAVPVTQTVTAPVIPSN
                     TVSTASPIITEPATTVISIEPANPDVVYIPNYNPTVVYGNWANTAYPPVYLPPPAGEP
                     FIDSFVRGFGYSMGVATTYALFSSIDWDDDDHDHHHHDDDDYHHHDGGHRDGNGWQHN
                     GDNINIDVNNFNRITGEHLTDKNMAWRHNPNYRNGVPYHDQDMAKRFHQTDVNGGMSA
                     TQLPAPTRDSQRQAAASQFQQRTHAAPVITRDTQRQAAAQRFNEAEHYGSYDDFRDFS
                     RRQPLTQQQKDAARQRYQSASPEQRQAVREKMQTNPQNQQRREAARERIQSATPEQRQ
                     VFKEKVQQRPLNQQQRDNARQRVQSASPEQRQVFREKVQESRPQRLNDSNHTVRLNNE
                     QRSAVRERLSERGARRQER"
     gene            complement(24643..26067)
                     /gene="lpdA"
                     /locus_tag="RH057_000247"
     CDS             complement(24643..26067)
                     /gene="lpdA"
                     /locus_tag="RH057_000247"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HDV3126671.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(26392..28284)
                     /gene="aceF"
                     /locus_tag="RH057_000248"
     CDS             complement(26392..28284)
                     /gene="aceF"
                     /locus_tag="RH057_000248"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HDV3126672.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEAAPAAAPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAAPAAKAEGKSEFAENDAYVHATPLIR
                     RLAREFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKV
                     DFSKFGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAK
                     RKLDVKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVV
                     PVFKDVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVN
                     APEVAILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSD
                     IRRLVM"
     gene            complement(28299..30962)
                     /gene="aceE"
                     /locus_tag="RH057_000249"
     CDS             complement(28299..30962)
                     /gene="aceE"
                     /locus_tag="RH057_000249"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HDV3126673.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKEISTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(31123..31887)
                     /gene="pdhR"
                     /locus_tag="RH057_000250"
     CDS             complement(31123..31887)
                     /gene="pdhR"
                     /locus_tag="RH057_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HDV3126674.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMAGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            32428..33801
                     /gene="aroP"
                     /locus_tag="RH057_000251"
     CDS             32428..33801
                     /gene="aroP"
                     /locus_tag="RH057_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HDV3126675.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAIFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQATVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(33844..34698)
                     /gene="ampE"
                     /locus_tag="RH057_000252"
     CDS             complement(33844..34698)
                     /gene="ampE"
                     /locus_tag="RH057_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HDV3126676.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAALFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(34695..35246)
                     /gene="ampD"
                     /locus_tag="RH057_000253"
     CDS             complement(34695..35246)
                     /gene="ampD"
                     /locus_tag="RH057_000253"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HDV3126677.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRPDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDCYPDIAKNMTGHCDIAPD
                     RKTDPGPAFDWARFRVLVSKETT"
     gene            35334..36227
                     /gene="nadC"
                     /locus_tag="RH057_000254"
     CDS             35334..36227
                     /gene="nadC"
                     /locus_tag="RH057_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HDV3126678.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     SLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIASGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            36430..36870
                     /gene="ppdD"
                     /locus_tag="RH057_000255"
     CDS             36430..36870
                     /gene="ppdD"
                     /locus_tag="RH057_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HDV3126679.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSAIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTHYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWTRNCNIQSDSALQQACEDVFRFDDAN"
     gene            36880..38265
                     /gene="gspE"
                     /locus_tag="RH057_000256"
     CDS             36880..38265
                     /gene="gspE"
                     /locus_tag="RH057_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspE"
                     /protein_id="HDV3126680.1"
                     /translation="MNIPQLTALCLRYQGVLLDASEEVVHVAVVDAPSHELLDALHFA
                     TTKRIEITCWTRQQMEGHASRTQQTLPVAVQEKHQPKAELLARTLQSALEQRASDIHI
                     EPADNAYRIRLRIDGVLHPLPDVSPDAGVALTARLKVLGNLDIAEHRLPQDGQFTVEL
                     AGNAVSFRIATLPCRGGEKVVLRLLQQVGQALDVNTLGMQPLQLADFAHALQQPQGLV
                     LVTGPTGSGKTVTLYSALQTLNTADINICSVEDPVEIPIAGLNQTQIHPRAGLTFQGV
                     LRALLRQDPDVIMIGEIRDGETAEIAIKAAQTGHLVLSTLHTNSTCETLVRLQQMGVA
                     RWMLSSALTLVIAQRLVRKLCPHCRRQQGEPIHIPVNVWPSPLPHWQAPGCVHCYHGF
                     YGRTALFEVLPITPVIRQLISANTDVESLETHARQAGMCTLFENGCLAVEQGLTTFEE
                     LIRVLGMPHGE"
     gene            38255..39457
                     /gene="hofC"
                     /locus_tag="RH057_000257"
     CDS             38255..39457
                     /gene="hofC"
                     /locus_tag="RH057_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HDV3126681.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRALLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEVFPPLYQAMIRTGELTGKLDECCFELARQQKAQRQLTEKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(39492..40535)
                     /gene="guaC"
                     /locus_tag="RH057_000258"
     CDS             complement(39492..40535)
                     /gene="guaC"
                     /locus_tag="RH057_000258"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HDV3126682.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHYSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGPVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVQEQENRIFNNL"
     gene            complement(40691..40735)
                     /locus_tag="RH057_000259"
     CDS             complement(40691..40735)
                     /locus_tag="RH057_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3126683.1"
                     /translation="MIIKTLKMSARKRN"
     gene            40760..41380
                     /gene="coaE"
                     /locus_tag="RH057_000260"
     CDS             40760..41380
                     /gene="coaE"
                     /locus_tag="RH057_000260"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HDV3126684.1"
                     /translation="MRYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGANMIAADGTLQRRALRERIFANPEEKNWLNALLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            41380..42123
                     /gene="zapD"
                     /locus_tag="RH057_000261"
     CDS             41380..42123
                     /gene="zapD"
                     /locus_tag="RH057_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HDV3126685.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELDRQQRKLQTWIGVPGVDQSRIEALIQQLKAAGSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            42133..42330
                     /gene="yacG"
                     /locus_tag="RH057_000262"
     CDS             42133..42330
                     /gene="yacG"
                     /locus_tag="RH057_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HDV3126686.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(42430..42819)
                     /gene="mutT"
                     /locus_tag="RH057_000263"
     CDS             complement(42430..42819)
                     /gene="mutT"
                     /locus_tag="RH057_000263"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HDV3126687.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGEPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(42879..45584)
                     /gene="secA"
                     /locus_tag="RH057_000264"
     CDS             complement(42879..45584)
                     /gene="secA"
                     /locus_tag="RH057_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HDV3126688.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPIAEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(45646..46158)
                     /gene="secM"
                     /locus_tag="RH057_000265"
     CDS             complement(45646..46158)
                     /gene="secM"
                     /locus_tag="RH057_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HDV3126689.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(46389..47306)
                     /gene="lpxC"
                     /locus_tag="RH057_000266"
     CDS             complement(46389..47306)
                     /gene="lpxC"
                     /locus_tag="RH057_000266"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HDV3126690.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(47407..48558)
                     /gene="ftsZ"
                     /locus_tag="RH057_000267"
     CDS             complement(47407..48558)
                     /gene="ftsZ"
                     /locus_tag="RH057_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HDV3126691.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(48619..49881)
                     /gene="ftsA"
                     /locus_tag="RH057_000268"
     CDS             complement(48619..49881)
                     /gene="ftsA"
                     /locus_tag="RH057_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HDV3126692.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(49878..50708)
                     /gene="ftsQ"
                     /locus_tag="RH057_000269"
     CDS             complement(49878..50708)
                     /gene="ftsQ"
                     /locus_tag="RH057_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HDV3126693.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLGWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLARFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(50710..51630)
                     /gene="ddlB"
                     /locus_tag="RH057_000270"
     CDS             complement(50710..51630)
                     /gene="ddlB"
                     /locus_tag="RH057_000270"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV3126694.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSMDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(51623..53098)
                     /gene="murC"
                     /locus_tag="RH057_000271"
     CDS             complement(51623..53098)
                     /gene="murC"
                     /locus_tag="RH057_000271"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HDV3126695.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLMNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMRVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(53152..54219)
                     /gene="murG"
                     /locus_tag="RH057_000272"
     CDS             complement(53152..54219)
                     /gene="murG"
                     /locus_tag="RH057_000272"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HDV3126696.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHHLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(54216..55460)
                     /gene="ftsW"
                     /locus_tag="RH057_000273"
     CDS             complement(54216..55460)
                     /gene="ftsW"
                     /locus_tag="RH057_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HDV3126697.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSSVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(55460..56776)
                     /gene="murD"
                     /locus_tag="RH057_000274"
     CDS             complement(55460..56776)
                     /gene="murD"
                     /locus_tag="RH057_000274"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HDV3126698.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDAECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALAA
                     LALADAAGLPRASSLKALTTFAGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     QVDGTLHLLLGGDGKSADFSPLARYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(56779..57861)
                     /gene="mraY"
                     /locus_tag="RH057_000275"
     CDS             complement(56779..57861)
                     /gene="mraY"
                     /locus_tag="RH057_000275"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HDV3126699.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(57855..59213)
                     /gene="murF"
                     /locus_tag="RH057_000276"
     CDS             complement(57855..59213)
                     /gene="murF"
                     /locus_tag="RH057_000276"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HDV3126700.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPARV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGTEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSVGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(59210..60697)
                     /gene="murE"
                     /locus_tag="RH057_000277"
     CDS             complement(59210..60697)
                     /gene="murE"
                     /locus_tag="RH057_000277"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HDV3126701.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVEQGATFCAMEVSSHGLVQHRVAALKFAASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     IDVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGVIA"
     gene            complement(60684..62450)
                     /gene="ftsI"
                     /locus_tag="RH057_000278"
     CDS             complement(60684..62450)
                     /gene="ftsI"
                     /locus_tag="RH057_000278"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HDV3126702.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNPDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALAMPSSALVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(62466..62831)
                     /gene="ftsL"
                     /locus_tag="RH057_000279"
     CDS             complement(62466..62831)
                     /gene="ftsL"
                     /locus_tag="RH057_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HDV3126703.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(62828..63769)
                     /gene="rsmH"
                     /locus_tag="RH057_000280"
     CDS             complement(62828..63769)
                     /gene="rsmH"
                     /locus_tag="RH057_000280"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HDV3126704.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEERF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(63771..64229)
                     /gene="mraZ"
                     /locus_tag="RH057_000281"
     CDS             complement(63771..64229)
                     /gene="mraZ"
                     /locus_tag="RH057_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HDV3126705.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLSSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
     gene            complement(64831..65835)
                     /gene="cra"
                     /locus_tag="RH057_000282"
     CDS             complement(64831..65835)
                     /gene="cra"
                     /locus_tag="RH057_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HDV3126706.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(65917..66003)
                     /locus_tag="RH057_000283"
     CDS             complement(65917..66003)
                     /locus_tag="RH057_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HDV3126707.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(66015..66506)
                     /gene="ilvN"
                     /locus_tag="RH057_000284"
     CDS             complement(66015..66506)
                     /gene="ilvN"
                     /locus_tag="RH057_000284"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV3126708.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(66509..68233)
                     /gene="ilvI"
                     /locus_tag="RH057_000285"
     CDS             complement(66509..68233)
                     /gene="ilvI"
                     /locus_tag="RH057_000285"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HDV3126709.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPGATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVWPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     TAGCHQQLKETVEALNLPVVSSLMGLGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRARQCLKYDTHSEKIKPQAVIETLWRLTKGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            68352..68396
                     /locus_tag="RH057_000286"
     CDS             68352..68396
                     /locus_tag="RH057_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3126710.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(68551..69495)
                     /gene="leuO"
                     /locus_tag="RH057_000287"
     CDS             complement(68551..69495)
                     /gene="leuO"
                     /locus_tag="RH057_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HDV3126711.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
BASE COUNT        16985 a        18912 c        16950 g        16743 t
ORIGIN      
        1 tcttggtctg aatacttcag tcggtgcatt ttctttcgat gtgactcact ccaatgttcg
       61 tataccggat gataaaacat atcagggaca aagttatcgt gtttcctgga acaagttatt
      121 cgaagaaaca agtacttcac tgaatatcgc ggcctatcgc tattcgacac agaattacct
      181 tggtcttaat gatgcactaa ctctaattga tgaagtgaaa catcccgaac aagatcttga
      241 accgaaatcc atgcgtaatt actcgcgcat gaaaaatcag gttacggtca gtattaacca
      301 accgctaaaa tttgagaaaa aagattacgg ttcattttat ctttccggaa gttggtccga
      361 ttactgggct tccggacaaa atcgtagcaa ttactctatt ggctacagta acagtgcatc
      421 ctggggcagc tacagtgtca gtgcccagcg ttcatggaat gaagacggcg acactgacga
      481 tagcgtttat ctcagtttca ccattcctat tgaaaaatta cttggcactg aacaacggac
      541 ttcaggtttc cagagtattg atactcaaat gagcagtgac tttaagggta ataaccaact
      601 caacgtcagt agcagtggct atagtgataa cgctcgcctc agttatagcg taaatactgg
      661 ctatacgatg aataaagcca gcaaagattt gagttatgtt gggggttatg ccagctatga
      721 atcaccgtgg ggaaccctgg caggttcagt ttctgcaaat agcgataaca gtcgtcaagt
      781 gtctctcagc accgacggtg gttttgtatt gcatagcggc ggcctgacct tcagtaatga
      841 cagttttagc gactccgata cactggcggt agttcaggct ccaggtgctc aaggagcgcg
      901 aataaattat ggcaacagta ctatcgatcg atggggttat ggtgtcacca gcgctctttc
      961 tccttatcat gaaaaccgta tcgcgctgga tatcaacgat cttgagaacg atgttgaatt
     1021 aaaaagtacc agtgcagtag ctgtaccgcg tcagggttca gtcgtctttg ctgattttga
     1081 aaccgtgcaa gggcaatcag ccattatgaa catcacacga agtgatggta aaaatattcc
     1141 atttgctgca gatatttatg atgagcaagg caatgtcatt ggtaatgttg gacagggtgg
     1201 acaagcattt gttcgtggca ttgagcagca gggaaatatc agcattaaat ggctcgaaga
     1261 aagtaaaccc gtaagttgtc ttgcgcatta tcaacaaagt tcagaagcag aaaaaatagc
     1321 gcaatctatt attctgaatg gaattcggtg tcagattcag taactacaag ggacgtcaaa
     1381 tgataaaaat aacgccacat aaaatagcga tactgatggg aatattatta tcaccatcag
     1441 tatttgcaac ggatattaat gtagagttta cagccactgt caaagcgaca acctgtaaca
     1501 tcacacttac tggtaataac gtcacgaatg atggcaataa taactacaca ttgagaatcc
     1561 ctaagatggg tctggataag atcgcgaata aaacgacaga atctcaggct gattttaaac
     1621 tggttgctag tgggtgcagc agtggcatca gttggattga taccactctg accggaaatg
     1681 catcatcaag ctcacctaag cttattatac cgcagtctgg tgattcatct tcgacgacaa
     1741 gtaatatcgg tatgggtttc aaaaaacgga ctactgatga tgccactttc cttaaaccta
     1801 acagtgcgga aaagatacgc tggagcacag acgagatgca gcccgataag ggtcttgaaa
     1861 tgaccgttgc gctacgtgaa acagatgcag ggcaaggcgt accggggaat tttcgtgcac
     1921 tggcaacgtt taatttcatc tatcaataag aaggataatt aagatgacat ttaaaaattt
     1981 acgttatgga ttatccagca gtgtcgtttt ggctgcctca ttgttcagcg taccctctta
     2041 tgcggcaaca gacagcattg ggctgaccgt gattactacc gtagaaatag gtacttgtac
     2101 cgctacatta gtaaatgact ctgatcagga catttctgtt gttgactttg gcgatgtata
     2161 tatttctgaa atcaatgcca agaccaaagt taaaacattc aaactcaaat tcaaagactg
     2221 tgcgggtatc cccaataaaa aagcacaaat aaaattaacc aagcgagcca catgcgaagg
     2281 tactgctaat gacggtgcgg ggttcgcaaa tggatccaca gccacagata aagcaagtgc
     2341 cgtcgccgtt gaagtctgga gcaccgaaac tccggcaaca ggtagcgcaa cacaatttag
     2401 ttgtgtgaca ccagcatctc aagaggtcac tatctccaat gcagccaacg cggttgttta
     2461 ttatccgatg agtgctcgac tggtcgtcga aaaaaataaa accgtaagca atgtcactgc
     2521 gggtaaattt tctgcacctg ccacatttac agtaacctat aactaacaga ctgacagatc
     2581 caggaactat atatgcatcc cactcaacgt aagctgatga agagaataat tctgtttctg
     2641 tcactacagt ttcccattac ctatccagcc atcgctgggc aggatattga tcttgttgcc
     2701 aatgtaaaaa acagcacttg caaaagcgga atcagtaacc agggtaatat tgatcttggc
     2761 gtcgttgggg tgggatattt ttcagataat gttactcctg aaagctatca accaggtgga
     2821 aaagagttca ctgtcacagt atcagactgt gcattacagg gaactggcga tgtgcttaat
     2881 cagttacata ttgattttag agcccttagc ggtgtcatgg ctgcaggttc aaggcaaata
     2941 tttgccaatg aagtcgcaac cggggctaaa aatgtcggtg tcgttctctt ttccattcag
     3001 gacccgacca atatattcaa tgttattagt tcagcaggaa attctcgttc cgtgtatcca
     3061 gtaatgactt ctgccctgca taattcttca tggaaatttt atgccagaat gcaaaaaatc
     3121 gatcccgcgc tggatgtcat atctggccag gtaatgagtc atatattagt tgatgtgtac
     3181 tacgaataat agatcaaaac aaataactgc gactgttaca gcagtagatg gtggagtgat
     3241 aatgaagaaa ttcttcagac attttctttt tttaattctg tgcttgagtt gttataccgc
     3301 gagtgctggc actgatgata acgtaggata tattgttggg aatagttacg gcgttggtcc
     3361 gtcagatcaa aaatggagag aaacggggcc gaatggcgat gctactgtca tattcagata
     3421 tgcaacaagt acaaataatt tagttttcta caaaccgacg cagcttggac ctacaggtgt
     3481 aaaattacag tggagtcagt tagataccgc ttctggtggt ggttttcttt attgcaacag
     3541 aagtgatagc acaagtggta gcgcaatgcg tattgaaaat gcaatggttg actcaggtaa
     3601 aatgtatggc tcccataaat tatttaatac atcagttcct ggtttgtatt acacattatt
     3661 aatttcaaac atgtggtcag cttacggtac cgtaactaac gttagttcac ctgggatata
     3721 cattggtgac tctgcagaac aatatttttc gtggtataat ccaagcgaag acgtgttata
     3781 ttggagttgc aataatgcga atagcacccg taaatactgg gctgtaggtg gtatttatca
     3841 gacccttaca attgaattct atacagatac aaactttgat ccaacagtaa ctcaacagat
     3901 caaattatcc agctcatcaa attatctgta ttcatttaaa gcctatggcg caggccaagg
     3961 tataaatgag catagttatt ttatcaaaat cgatttcgat ctgctcaatg tcaagttgac
     4021 taatcccact tgttttaccg ctatgcttag tggaacttct gtgactggtt ctacggtaaa
     4081 aatgggtgaa tatagtgcag aacaaatcag aaacggtgcc acaccggttc cttttgatat
     4141 ttcacttcaa aactgcgttc gtgtgactaa tattgagaca aaattagttt caacaaaggt
     4201 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgacg cagcaaaagg
     4261 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
     4321 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
     4381 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
     4441 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
     4501 ggtaacctac ccttaataag tccaccgcac cgccatcatc tggcggtgcg gtaattgata
     4561 aatcattgcc cccaccaatg tgactgataa aatgccaggc aacacgacat catttatcag
     4621 gacacgttat gaaaccgacc accattgctt cacttcagaa atgtaagcag gataaaaagc
     4681 gtttcgcgac catcaccgct tacgactaca gcttcgcgaa actatttgct gatgaaggac
     4741 ttaacgtcat gctggtaggc gattcgctgg gcatgacggt tcagggccac gactccaccc
     4801 ttcccgttac cgtcgaggat atcgcctatc ataccgccgc cgtacgtcgc ggcgcgccaa
     4861 actgcctgct gttggctgac ctgccgttta tggcgtatgc cacgccggaa caagcctttg
     4921 aaaacgccgc aacggttatg cgtgccggtg ccaatatggt caaaattgag ggcggtgagt
     4981 ggctggtcga aaccgtaaaa atgctgaccg aacgtgccgt tcctgtatgt ggtcacttag
     5041 gtttaacccc acagtcagtg aatattttcg gtggctacaa agttcagggg cgcggcgatg
     5101 aagcaggcga tcaactgctc agcgatgcat tagccttaga agccgctggg gcacagctgc
     5161 tggtgctgga atgcgtgccg gttgaactgg caaaacgtat taccgaagca ctggcgatcc
     5221 cggttattgg cattggcgca ggcaacgtca ctgacgggca gatcctcgtg atgcacgacg
     5281 ccttcggcat taccggcggt cacattccta aattcgctaa aaatttcctc gccgaaacgg
     5341 gcgacatccg cgcggctgtg cggcagtata tggctgaagt ggagtccggc gtttatccgg
     5401 gcgaagaaca cagtttccat taaggagtca cgttgtgtta attatcgaaa ccctgccgct
     5461 gctgcgtcag caaattcgcc gcctgcgtat ggaaggcaag cgcgtggcgc tggtgcctac
     5521 tatgggtaat ctgcacgatg gccatatgaa gctggtcgac gaagccaaag cccgcgccga
     5581 tgtggtcgtc gtcagtattt tcgttaaccc gatgcagttc gaccgcccgg aagatctggc
     5641 acgttatcca cggaccttgc aggaggactg cgagaagcta aacaaacgta aagtggattt
     5701 agttttcgcc ccttcggtaa aagagatcta cccgaacggt actgaaaccc atacttacgt
     5761 tgacgttccc gggctttcga ccatgctgga aggcgcgagc cgtccggggc attttcgcgg
     5821 cgtttcgact atcgtcagca agctgtttaa cctggtccag ccggacatcg cctgcttcgg
     5881 tgaaaaggac tttcagcaac tggcgctgat ccgcaaaatg gttgccgata tgggcttcga
     5941 tattgagatt gtcggtgtgc caattatgcg cgccaaagac ggtctggcgc taagttcccg
     6001 taacggttat ctgacggcgg aacaacgcaa aattgcgcct ggtctgtaca aagttttaag
     6061 ttcgattgct gacaaattgc aggctgggga acgggatctc gatgaaatta ttaccattgc
     6121 ggggcaagaa ctgaatgaaa aaggcttccg cgccgatgat attcagattc gcgatgccga
     6181 cacattgctg gaagtttctg aaaccagcaa acgggcagta attctggtag ccgcctggct
     6241 tggcgatgct cgcctgatcg acaacaaaat ggtcgagctg gcgtaatact taactggcgc
     6301 tacggctgat ggcgccagtt attaatttac cccacgtcat ctgtcataac acaggtaact
     6361 taaataaggt taattacctt atcaatctca gcaagagata aatttgtcat ctctgcaacg
     6421 tcttcccgag acattccttt acttagaaga cgcagggcga attcttgcct ttcctcctgt
     6481 cttccctgct gaatcccctt ctcaatccct ttctcttcaa accactgcgc cagcgtcatc
     6541 atagactccc ctcccgtttc cctgtctctc agcacaccat aaaacaaatc cgcttgttca
     6601 gtatgaccgc gttgcagcat atagttttgc atggcaacta actgacttcc gctagtgtac
     6661 ccttcgtcaa tcagcgtgac cagttgctcc agcaataaca ttaagtcgcg ctggcgaata
     6721 tgtttttgca gtagttcgag aatcgcaatc cgccgatgtt gcatgatttc gtcatccggt
     6781 gtgatggtga tatccaccag cgggaaagga ctgttataga cgcgtcgtgc cagctccggc
     6841 gagtaaaata tatcaaacca gcacattgag agcggataag gtgtggcctc gccctgataa
     6901 aacagtatcg gcaccaccag cggcagctta tcgtgatccg cctccagatg ccggtgcatg
     6961 gcagcaatag aataacgcat catgcgaaag gccattttct tatccggctt gctttggtgt
     7021 tcaatcacaa catgcagata accgggattg ccctgcattt gcacggaata gagcacgtcc
     7081 gtgctgtgtc ctttcaggct ctcttcaatg aaactccccg actctaaatg aagcgtgttg
     7141 aggtcacaaa gttctcgtaa ttccactggc aaatgtatct ccagaaagtc gcgagccgtc
     7201 tccgcatgca ttaaaaattg tttaaatacc gcgtcatgcg gtgtggtact cggtgcatcc
     7261 atcttccctg tcttccgtgt tgtcttgcgt gaaggcagta tccagtcaag gcgcgccgtc
     7321 gtcagccgcc tctttagtgt ttaccgagca gcgttcagag aaatttcaat tcgttacagg
     7381 cgatacatgg cacgcttcgg cgcggtacgc aaactgctga cgcaaatgcc atcagatgag
     7441 tagacactca tcaaaaatcg ggcaatactg cgtgagaatt tcctcaaagc aggcaccgcc
     7501 tgcttcgtta acgacagggt agaaaggtag aagttatgat tcgcacgatg ctgcagggca
     7561 aactccaccg cgtgaaagtg actcatgcgg acctgcacta tgaaggttct tgcgccattg
     7621 accaggattt tcttgacgca gccggtattc tcgaaaacga agccatcgat atctggaatg
     7681 tcaccaacgg caagcgtttc tccacttatg ccatcgcggc agaacgcggt tcgagaatta
     7741 tttctgttaa cggtgcggcg gcccactgcg ccagcgtcgg cgatattgtc atcatcgcca
     7801 gcttcgttac catgccagat gaagaagctc gcacctggcg acccaatgtc gcctattttg
     7861 aaggcgacaa tgaaatgaaa cgtaccgcga aagcgattcc ggtacaggtt gcttgattgt
     7921 tatccctgcg gctggttact catcagccgc gacatcgtct ccagcgaatc cgttcttaag
     7981 atataaagtc gttttagtaa caacggatta tcccccggtt ttactttgcc tttcatggtt
     8041 gtcaccgcca ggtgaaatcc ggcatcgttt gctgccttca cggctttgtc attaaatccg
     8101 ccaaacggat acgaaagata ccagacatgc ggattaaatt gcgccagagc gcggcgtgaa
     8161 cgtgcaaaat caaacagaat attgtgctca ctacggctca gtaatatggg tcggcgatag
     8221 ccatccaccc gatgcaaaaa atgggtatgt gactgaaaat caaaaacatc gcgaatttcg
     8281 ttaagctcag aaacgctcat aaattgcagc gattttgggt tccacttctg cgggtgacgt
     8341 ttgatgcgcg aggtaacaac aaacgccgtc gccttcatgc catattgttt caatacagga
     8401 tacgcatagc ggctcaccga cttgaggcca tcatcaaagg taatcaccac cgctcgcgca
     8461 gggagattga tcttattctt cacgtaacct tccaactgcg ccatgctcag tgtcgcgtat
     8521 cccctgtcgc gcagccaggc catctggtta ttgaaagcgc gtaccgaggt ggtcgtcgaa
     8581 gtatggcgaa aacgggtgtt ttcttcatcg cgcagaatat ggtgataggt tagcaccggc
     8641 aggccattat cgggttgggc atccagcgcg ctgatatagg cgagtcgatc gccaatacgg
     8701 atctgatacc aggtttgatt taacctgtct ttcagtttat gcaaaatcgg gtagcgcaaa
     8761 ttgtccgcca gtaccccaaa tggcgcactg cccgcactcg gcgcgttata gaccggcgta
     8821 tctttccagg taattaagtt ctgattactc agcggcttgt tgaggtcacc caaaccgtct
     8881 tcaacttttt gtcgcccctg aactggctcg agatgccctt tatcgataaa ccctttacca
     8941 aagccaaaat taaatgcgta ataacttgcg gcagtgggct ccacggcaat gatttgcccg
     9001 gcccgaatat tccccacggt caccatcttg tcgccaattt gcgcccagat cgcagcattt
     9061 tcgatggttt gcatataacg ggcaggtaac gcggcactga cacttgcggt aaacagcatc
     9121 agcaggagaa taacagcttg tttgtacatg aaaaaataac cgagggtgaa acaacgcttt
     9181 tattgtagca aaagcgctat aagaatcgtg ggttaatact catacaaatt atgtttgagg
     9241 acgaaaggcg gatttttttg ttgttggtgc caaagactac tcacctccgg cccacccagt
     9301 tcgacaatac gctcgcgaaa ctctgaactg gtcatggttt cacggcaagc catcatctgt
     9361 tcaattaacg gtaacgtgac accagaaatc acttcgcaac gggagtgttt gtggcttaat
     9421 aatgaagcca cgcggtacgg tggcgctccg gctatatcgg ttaagaagat gacaccctca
     9481 ccagagtccg cttcatgcag agcatcgcac atcatgcggc tgagcatatt agagcttaac
     9541 ccacgccaga aattcacggc ccggcactga agaagcgccc catgtttttt ctccagcgca
     9601 tccagtatct cttgcgcccg gtcatcgtga caggtaatta cccaacctaa catcgccttc
     9661 ctccttagcc gaaaatcagt ctaccctgac cattgttgat aaagggtgat agccgtcaaa
     9721 actcctctcc cccaaatcca gaggagagga aatagcctta gctacgcaaa ccacgtccac
     9781 gttggatcag cgaccaacag atcaaataaa acgccacaat aaagaccacc agtacgccaa
     9841 aggtagtgac cagcggaaca tcattgatac cgaggaagcc gtagcggaaa ccactgatca
     9901 tataaacgat tgggttcagg tgcgacagcc cttgccagaa cggcggcaac aaagtcagtg
     9961 agtaaaagac cccacccaaa tacgtgagtg gcgttaacac aaaggttggc accaggctga
    10021 tgtcatcgaa cgttttggca aacacaccgt tcagcaaacc cgcaagggag aacaacaccg
    10081 ccgtgagcac cagggttaag gcaacgaata cccacgaatg cacctgaaac ggcacaaaaa
    10141 acagtgaaat tgccgtcacc agaatgccaa caaacagacc acgcgccacg ccaccgccga
    10201 catatccggc aataatgacg tgagtcggaa ccggcgctac cagcagctct tcaatattac
    10261 gctggaactt ggcaccaaaa aatgatgacg caacgttggc gtaggcattg gtgatcaccg
    10321 acatcatgat cagccccggt acgatgaact gcatatagct gaagccatgc atatcgccaa
    10381 tacgcgaacc aatcaggtta ccgaagataa taaagtaaag ggtcatggtg atgactggcg
    10441 gcaccagcgt ctgcacccag atacgcataa agcgatggat ctctttcgcc cagatgcttt
    10501 ttagcgccac ccagtaaaga tgcatcatgc gcgatctcct tgtttttcat taaccagtga
    10561 aacaaacagc tcttccagac ggttagcttt gttacgcata cttaatacct gaatgccctg
    10621 ctcacttaac tgcgtaaata cgctgttgat cccctgctca cgcagcactt caacttccag
    10681 cgtcgcggta tcgaccagtc gatactgata gccatcgagc ttcggtaacg gacttttcgg
    10741 tgcgagatcg agaataaagg tttccgattt cagcttcgcc agcagcgcct tcatcgaggt
    10801 attttccacc agctcaccgt gttgaataat gccgatattg cggcacagca tttctgcttc
    10861 ttccaggtag tgtgtggtga gaatgatggt ggtgcctttg tcgtttaaat ccttcaaaaa
    10921 gccccacatt gagcggcgaa gttcaatatc cacgcctgcg gtcggttcgt cgagaatcag
    10981 tagtttaggt tcatgcatta acgcacgggc aatcattaaa cggcgcttca tcccgccaga
    11041 taacatacgc gcacgttcgt tgcgttttcc ccatagatcg agttgtttaa gatatttttc
    11101 gctgcggatg tacgcctctt tgcgctccac accgtagtac cctgcctgat ttaccacaat
    11161 ttgctgcacg gtttcaaacg ggttgaagtt aaattcctgc ggcaccagtc ccaactgacg
    11221 tttagcgttc acgacatctt tctcgagatc gtaaccaaat acgctgaccc gcccggaggt
    11281 tttatttacc agagagctga taataccgat agtggtcgat ttcccggccc cgttcggccc
    11341 gagaagcgca taaaaatcac ccgcttcgac ctgcaaatct attccacgaa gcgcctgaac
    11401 gccgcctgga taagtttttt taagctgttg aagttccagt gcaatggtca taaattttta
    11461 cttaccttac gttcttacac tttatctgtg gtttaaatcg tcccggagtt gccctatatt
    11521 agccaaacgt aattatttgg ttacaggtcg ttaacctcca tgaaagacat agatacactc
    11581 atcagcaaca atgcactatg gtcaaaaatg ctggtggaag aggatcccgg gttttttgag
    11641 aaactggcac aagcgcaaaa accgcgcttt ctatggattg gatgttccga cagtcgcgtt
    11701 cctgcagaac gtttaaccgg tcttgagccg ggcgaactct ttgttcaccg taatgttgct
    11761 aacctggtca ttcacaccga cctgaactgc ctttccgtgg ttcagtatgc agtggatgta
    11821 ctcgaagttg aacacattat tatctgtggc cactacggtt gcggcggcgt acaagccgca
    11881 gttgaaaacc cggaactggg gcttatcaac aactggctgc tgcatatccg cgatatctgg
    11941 ttcaaacata gctcattgct cggcgaaatg ccgcaagagc gccgtctgga taccttgtgt
    12001 gaactgaacg tcatggaaca ggtttataac ctgggccact ccaccattat gcaatcagcg
    12061 tggaaacgcg ggcagaaagt taccattcac ggctgggcct acggcattca cgacggcttg
    12121 ctgcgtgatc tggatgtcac agcgaccaac cgcgaaaccc ttgagcaacg ttaccgtcac
    12181 gggatttcca acctcaagct gaaacacgcc aaccacaaat aaaaatgcca tgccggatgc
    12241 aacacatccg gcaacttcac acttactcgt ccagcagaat cactttgccg atatacggca
    12301 gatgacggta acgctgtgcg taatcaatgc cgtaacccac cacaaactca tccgggatcg
    12361 agaaaccgat aaattcaacc gggacgttca cttcacgacg ggacggttta tccagcagcg
    12421 tacaaatcgc cagcgacttc ggttcgcgca ggcttaagat ctcacgcact ttcgacagtg
    12481 tattccccga gtcgatgata tcttcaacaa tcagcacgtc cttgccacgg atatcttcat
    12541 ccagatcttt gaggattttc acatcacggg tggtggacat gccgctaccg tagctggagg
    12601 cggtcataaa gtcgacttca tgagatacct gaacttcacg gcacaggtcc gccataaaca
    12661 taaatgagcc acgcagcaga ccaaccagca ccatatcgct gccgctgtct ttgtaacgct
    12721 cagtaatctg acgacccagt tcggcgatac gcgctttaat ctccgcttcg gggatcatta
    12781 cttctacagt atgtttcata tctctaacca tatgatttaa aaacaaatca cttcgcacgg
    12841 gcaggttttg gcaccgcaac aaagcgcaag acacgcagta taccagtaaa actattcatt
    12901 cgcggaatct gttaataaag ctcgaattgt gatgacgatc acacatgtta aaacctataa
    12961 ttaattgcta ctaaaatatt aatgaattga aatggtgtct ctttatggca attaacaata
    13021 caggctcgcg acgattactc gtcacgctaa cagccctttt tgcagcgctt tgcgggctgt
    13081 atctactcat tggcggaggc tggctggtcg cgattggcgg ctcctggtac taccctatcg
    13141 ctggtcttgt gatgctcggc gtcgcctgga tgctgtggcg cagtaaacgt gccgcgcttt
    13201 ggctgtacgc agccctgctg ctcggcacca tgatttgggg cgtctgggaa gttggtttcg
    13261 acttctgggc gctgactccg cgcagcgaca ttctggtctt cttcggcatc tggctgatcc
    13321 tgccgtttgt ctggcgtcgc ctggtcattc ctgccagcgg cgcagttgcc gcactggtgg
    13381 tggcactgct gattagcggt ggtatcctga cctgggccgg atttaacgat ccgcaggaaa
    13441 tcaacggcac cttaagcgcc aatgccacac ctgcggaagc tatctcaccc gttgccgatc
    13501 aggactggcc tgcctatggt cgcaatcagg aaggtcaacg cttttcacct ctgaaacaaa
    13561 ttaacgccga taacgtccat aatctgaaag aagcctgggt gttccgtact ggcgatgtga
    13621 agcagccgaa cgatccgggc gaaatcacca atgaagtgac gccgattaaa gtgggcgaca
    13681 ccctttacct gtgtaccgct caccagcgcc tgtttgcgct cgatgccgcc agcggcaaag
    13741 agaaatggca ttacgatcct gagctgaaaa ctaacgagtc tttccagcac gtaacctgcc
    13801 gtggcgtctc ttatcatgaa gccaaagcag aaacggcttc accggaagtg atggcggatt
    13861 gcccgcgtcg tatcattctt ccggtcaatg atggtcgact gattgcgatt aacgctgaaa
    13921 acggcaaact gtgcgaaacc ttcgccaata aaggcgtgct caatctgcaa agcaatatgc
    13981 cagacaccaa accgggtctg tatgaaccga cttcgccacc gattatcacc gataaaacca
    14041 tcgtgatggc cggttcagtt accgataact tctcaacccg cgaaacgtct ggcgtgatcc
    14101 gtggttttga tgtcaacacc ggggagctgc tgtgggcttt tgatcccggc gcgaaagatc
    14161 cgaacgcaat cccgtctgac gaacacacct ttacctttaa ctcgccaaac tcctgggcac
    14221 cagcggccta tgacgcgaag ctggatctgg tctatctgcc gatgggcgtg accacgccgg
    14281 atatctgggg cggtaaccgc acaccggaac aggaacgtta tgccagctcg attctggcgc
    14341 tgaatgccac taccgggaaa ctggcgtgga gctaccagac cgttcaccac gacctgtggg
    14401 acatggatct tccggcacag ccgacgctgg cggacatcac cgttaatggt cagaaagtgc
    14461 cagttattta cgctccggcg aaaaccggca acatttttgt gctcgatcgt cgcaatggcg
    14521 aactggtggt tccggccccg gaaaaaccgg tgccgcaagg tgctgcgaaa ggcgattacg
    14581 ttactccgac ccagccgttc tctgaactga gcttccgtcc gaagaaagat ttgagcggtg
    14641 cggatatgtg gggagccacc atgtttgacc aactggtgtg ccgcgtgatg ttccaccaga
    14701 tgcgctatga aggcattttc acgccgccat ctgaacaggg cacgctggtc ttcccgggta
    14761 acctgggaat gtttgaatgg ggcgggattt ccgttgatcc aaatcgtgaa gtggcaattg
    14821 ccaacccaat ggcactgccg tttgtttcga aactgatccc acgcggtccg ggtaacccga
    14881 tggagcagcc gaaagatgcc aaaggcacgg gtacggaatc cggcattcag ccacagtacg
    14941 gtgtaccgta tggtgtcacg ctcaacccgt tcctctcacc gtttggtctg ccgtgtaaac
    15001 agccagcatg gggttatatc tcggcgctgg atctgaaaac caatgaagtg gtgtggaaga
    15061 aacgtattgg tacgccgcaa gacagtatgc cgttcccgat gcctgtaccg gtgccgttca
    15121 atatgggtat gccgatgctg ggcgggccaa tctccacggc gggtaacgtg ctgtttatcg
    15181 ctgctacggc agataactac ctgcgcgctt acaacatgag caacggtgaa aaactgtggc
    15241 agggccgttt accagcgggt ggtcaggcga cgccaatgac ctatgaagtg aatggcaagc
    15301 agtatgtggt gatctccgca ggcggtcacg gttcatttgg tacgaagatg ggcgactata
    15361 ttgtggctta tgcgctgccg gatgatgtga aataaccata atgcccggag agatccgggc
    15421 atatttccga atacggtctt tttataccgt aaaccctaac atcatccccg tatcttcatg
    15481 ctccagcaga tggcagtgcg ccatataagc atgttctttc ggtgcgttgt gattaaactt
    15541 caccagcact tcactgacat tgccttctac cttaacggta tctttccagc ccgcgcgatg
    15601 agccgctggc ggtttgccat tttctgacaa gatacggaac tgggtgccgt gaatatggaa
    15661 cggatgcagc atcatgtcgc ccacgccaga gataacccaa cgttcgtact gccctttcgc
    15721 cgccgcaaac atcggcttat tcatatcaaa cgcctgaccg ttgattttat tggcatggtg
    15781 gaaatcgaac ttcccgccgt ggttcatatg attcatatta ccgtgcccca tatggcccat
    15841 catctggctg tgatccattc cggccatcgc ctgatcgcca tatttctcca ttagcatctg
    15901 catacccatc atatcgagca tcgggtccat agaaagttgc agcttgcgta ccgtcagccc
    15961 ttccagcgaa ggtaacgcag gcaggctact taatgtgtct ggcaaagcac cggaggcact
    16021 aatagcaatc ggctgaatcc gcattaccgg atgcggcttg tcaaacggcg caatcgccat
    16081 ccccatctgg ctgaccggta gcgtcaccag gtcaaagggt ttgttgtcgt taacctccac
    16141 cagcacttca aaacgctcgc ccatcagcac cggcagctcg ctcaccttca ccggttcagg
    16201 tagcagacca ccgtcgctgg caatcacata cagcgggcga ttgtcgctgg tggcgaaatt
    16261 gagcgagcgg gcattacagc cattgagcaa acgcaggcgc agccaaccac gcggggcagc
    16321 gtgttgtggg tagattgcac cgttggtcag cagcgtatcg ccaaaccagc ccacggcggc
    16381 ggtcatcaca tccagttgat aatcaatctg cccatcagcg ttaaattttt tatcctgaac
    16441 aatcaccgga acatcatcga taccccactg ttttggcagc attaatttca ggacctcgtc
    16501 atcttcaatt accaccagcc cagccaaccc catcgccacc tgtcgcccgg tcttgccatg
    16561 ttgatgtgga tggaaccagc aagtagcggc aggttgatca acgttcaacg tcaccgagcg
    16621 cttgccacct ggcagaataa ttccctgcgg gccgccgtcg acttcacccg gtacttccag
    16681 cccgtgccag tgcaacgtcg tctcttccgt cagttggtta tagatatcaa ccgttaccgc
    16741 tttgccgcgc tgtaatttca ccgccggccc cagcagattg ccgttatagc cccaggtagt
    16801 tgcagttttc tcgccaaagg tagactgacc tgcgccaata gttaactgaa tgcgattacg
    16861 ggcatcggtc gtgagcaaat cagggatcgg taacgttggg cgttccgccg caaagactgc
    16921 gcggctccac agcggcaagg ctgaagccac acccagcgcg acggaatatt ttaagaaatc
    16981 acgacgttgc atagttattt ccttattctt aagcaggcga aacaaaatca aacgttgagc
    17041 atagtccttc cccttacggg aaggtcaagc cgcagacaca atttaatcga caataatggt
    17101 cgtcgcctcg ctcgcagtgt gctaacgttt atcttcttta agcccctggt agaagcaatg
    17161 aagacgtttt tcagaacagt gttattcggc agcctgatgg ccgtttgcgc aaacagttac
    17221 gcgctcagcg agtctgaagc cgaagatatg gccgatttaa cggcagtttt tgtctttctg
    17281 aagaacgatt gtggttacca gaacttacct aacgggcaaa ttcgtcgcgc actggtcttt
    17341 ttcgcccagc aaaaccagtg ggatctcagt aattacgaca ccttcgacat gaaagccctc
    17401 ggtgaagaca gctaccgcga tctcagcggc attggcattc ccgtcgctaa aaagtgcaaa
    17461 gccctggccc gcgattcctt aagcctgctt gcctacgtca aataatccct gatacctttt
    17521 tgtagaaata ttgaccgtgc atccgcggtc aatgttagct attatgttgc gccctttttt
    17581 tacgggtgtt aacaaaggag gtatcaaccc atggccgaaa aaaaacagtg gcatgaaacg
    17641 ctacacgacc agtttgggca gtactttgcg gtagataacg ttctgtatca tgaaaagacc
    17701 gatcaccagg atctgatcat ttttgagaac gctgcatttg gtcgcgtaat ggcgctggat
    17761 ggcgtagtac aaaccaccga gcgcgacgag tttatctatc atgagatgat gacccatgtt
    17821 ccgctactgg cccatggtca cgcgaaacat gtgctgatta tcggcggcgg cgacggtgcc
    17881 atgctgcgtg aagtaacccg acataaaaac gttgagtcaa tcacgatggt ggaaatcgat
    17941 gcgggtgtcg tatcgttctg ccgtcagtat ctacccaacc ataacgccgg tagctacgac
    18001 gatccgcgct ttaagctggt gatcgacgat ggcgtcaatt tcgttaatca aaccagccag
    18061 acctttgatg tcattatctc cgactgcacc gatcctatcg gtcccggcga aagccttttc
    18121 acttcggcat tttatgaagg ctgcaaacgt tgcctgaatc ctggcggtat cttcgtcgca
    18181 caaaacggcg tctgcttttt acagcaggaa gaagccatcg acagccatcg caaactcagc
    18241 cattacttca gcgacgttgg cttttatcag gcggcgatcc cgacctatta cggcggtatc
    18301 atgacttttg catgggcgac agataacgac gccttacgcc atctctcaac cgaaattatt
    18361 caggcgcgtt ttctcgcttc tggcctgaaa tgccgttatt acaatccggc aatccatacg
    18421 gcagcttttg ccttacctca gtatctgcaa gacgcactgg cttcacagcc gtcctaagga
    18481 gaagataaga aattgaaaaa actgaaactg catggcttta ataatctgac caaaagtctg
    18541 agtttttgta tttacgatat ctgctacgcc aaaactgccg aagagcgcga cggttatatt
    18601 gcttatatcg atgaactcta taatgccaac cgtctgaccg aaatcctgtc agaaacctgt
    18661 tccattatcg gggccaatat tcttaacatc gcccgccagg attacgaacc acagggtgcc
    18721 agcgtcacta ttctggtgag tgaagaaccg gttgacccga aactcatcga caaaacagaa
    18781 caccccggcc cactgccaga aacggtcgtt gcccatctcg ataaaagtca tatttgcgta
    18841 catacctacc cggaaagtca tcctgaaggc ggtttatgta ccttccgcgc cgatattgaa
    18901 gtctctacct gcggcgtgat ttctccgctg aaggcgctga attacctgat ccaccagctt
    18961 gagtccgata tcgtaaccat tgattatcgc gtgcgcggtt ttacccgcga cattaacggt
    19021 atgaagcact ttatcgacca tgagattaat tcgattcaga actttatgtc tgacgatatg
    19081 aaggcgctgt atgacatggt ggatgtaaac gtctatcagg aaaatatctt ccataccaag
    19141 atgttgctta aagagttcga ccttaagcac tacatgttcc acaccaaacc ggaagattta
    19201 accgacagcg agcgccagga aattaccgct gcgctgtgga aagaaatgcg cgagatttat
    19261 tacgggcgca atatgccagc tgtttaacgg ctctggcgga gctcccaggc tccgccagat
    19321 ttatttactt ctgctgcacg aaattgcggt aagccgccac gacctgcaga aaatcctcaa
    19381 cgccgcatag cgacaggctt tcttcgtcgt agtagttcat cccctcttcc atttcatcgc
    19441 cagcgaattc cagttgattg gcgcgaacca tcacctcttc accgtccatc cacagggtgt
    19501 attcatgccc tgcccgttgc caggaccgtt cgctaccttt cagtgcgtgc gctgcttgtt
    19561 ccacttcatc aagcaaggcc aggttttctt tcacctcttc attaaaccag tgcccgacca
    19621 cttcatgccc catggacata cgcaccttta ccactccggt aatatcgcgc agaaattcgt
    19681 aatccatagt gtgttcctct gctcccggca atgcgccgaa atcgcattgc gccattgatg
    19741 taattatcgc agccttaccg gagaaaaaca gcggaacggc gatggtttag aaataaaaaa
    19801 atgcccggga atctcattca ccgggcattg tgtcgtttat gcgcagcgcg tgcgcttact
    19861 tattacaccg cagtctggaa aatcacccca tcggcttttt cggtgtactg agaaagctgg
    19921 ttgaagttca gataacgata agtatcaacg gcagttttat ctacttgcgc cacgtaggtc
    19981 tggtactctt ccggcgtcgg cagtttgcca atcagcgccg caacagccgc cagttccgca
    20041 gaagccagga agacattcgc gccagtaccc agacggttcg ggaagttacg ggtagaggtg
    20101 gaaaccaccg tcgcaccgtc tgctacacgc gcctggttac ccatacacag agaacagccc
    20161 gggatctcga tacgcgcacc actcttaccg aagacgctgt agtagccttc ttcagtcaac
    20221 tgcgcggcgt ccatacgggt tggcggtgcc acccacaggc gggtcggcag ttggcctttg
    20281 tgcgcatcca gcagtttacc cgcagcacgg aagtgaccga tgttggtcat gcaggaaccg
    20341 ataaatactt cgtcgatctt ctcaccctgt accgcagaca gcggacgcgc gtcatccggg
    20401 tcgttcggtg cacagaggat tggctcttta atatccgcca gatcgatgtc gatcactgcc
    20461 gcgtattcag catctgcatc ggcttccagc agctcaggat tcgccagcca tttttccatg
    20521 ccctgaatac gacgttccag ggtgcgacga tcgccgtaac cttccgcgat catccacttc
    20581 agcaggacga tgttagagtt caggtattcg atgatcggtt ctttgttcag cttgatggta
    20641 caaccagcgg cagaacgctc ggcggacgca tcggtcagtt caaatgcctg ctcaactttc
    20701 agatccggca gaccttcaat ttccaggatg cggccagaga agatgttttt cttgcctttc
    20761 ttctcaacgg tcagcagacc ttgtttgatc gcatacagcg gaatggcatg taccagatcg
    20821 cgcagggtga tgcccggctg cattttgcct ttgaagcgca ccagaacgga ttccggcata
    20881 tccagcggca ttacgcctgt tgcggctgca aatgccacca gaccagaacc cgccgggaaa
    20941 gagataccga tcgggaaacg ggtatgggaa tcaccaccgg taccgacggt atccggcagc
    21001 agcatacggt tcagccagga gtgaatgacg ccgtcacccg gacgcagcga cacaccgcca
    21061 cggttcataa tgaagtccgg cagcgtgtgg tgcgtgttca cgtcaactgg cttcggatac
    21121 gccgcggtgt ggcagaaaga ctgcatcacc aggtcagccg agaagcccag gcacgccagg
    21181 tctttcagtt catcacgggt catcgggccg gtggtgtcct gagagcctac agaagtcatt
    21241 ttcggttcgc agtacgcgcc cggacgaatg cctttcacgc cacaggcacg gcctaccatt
    21301 ttctgcgcca gcgagaagcc gcgatcgctc tcagcgacat ctttcgcctg acggaacaca
    21361 tcactgtgcg gcagaccaag tgcttcacgc gctttggtgg tcaggccacg cccgataatc
    21421 agcgggatac ggccgccagc acgcacttca tcaatcagca cgtcggtttt cagttcgaag
    21481 gtcgccagca gttcgcctgt ttcgtggtta cgcacttcac ctttgtacgg gtaaacgtca
    21541 atcacgtcgc ccatgttcag gttagagacg tcgacttcga ttggcagtgc acccgcgtct
    21601 tccatcgtgt taaagaagat gggtgcaatt ttaccgccga ggcacaaacc accgccgcgt
    21661 ttgttcggca catgtggaat atcatcgccc ataaaccaca gaacggagtt cgtggcggat
    21721 ttacgcgatg aacccgtacc cacaacgtca ccgacgtacg ccagcgggaa acctttctgt
    21781 tgcagagctt caatctgttt gatcgggcca acaacacctg gctggtctgg ctcaatacct
    21841 tcacgggcgt ttttcagcat cgccagcgcg tgcagtggga tatccgggcg cgaccacgca
    21901 tccggtgccg gagaaaggtc atcggtgttg gtttcgccag tgactttgaa gacggtaacg
    21961 gtcagttttt cagccagcgc cgggcgattc aggaaccatt cggcatccgc ccaggactgc
    22021 attacctgct tcgcatattc gttgcctgct ttcgctttct cttctacgtc atagaagtta
    22081 tcgaacatca gcagtgtgtg agaaagtgct ttggcagcga tcggtgccag tttggcatca
    22141 tccagcgcgt cgatcagcgg atgaatgttg taaccaccct gcatggtgcc cagcagttcg
    22201 atggcttttt ccggagtcag cagaggggat ttggcttcgc ctttcgcgat agcagccagg
    22261 aagcctgctt tgacataggc ggcttcatcg acgccagggg gaacacggtt ggttaacaga
    22321 tctaacagga attcttcttc gcccgcgggc gggtttttca gcagctctac aagtgcggcc
    22381 atttggtttg catccagggg tttgggcgca atcccctcag cggcacgctc agctacgtgc
    22441 ttacggtatt cttctagcac gacggttctc ctcgctctca ttgtcatagt gcggcaggcg
    22501 attctcttca cgctcctgtg agacagcagt ttgtagggta aatgcccagt accgcaacgg
    22561 gcagaatagc aggattttga cgatgtgtta atctgtttac aaaaaagcaa cataaaaaag
    22621 tggctgaatc gttaaggatg gtctagagat tgtttccctc cgtaaaaact tcgttaattc
    22681 gcatggtgat aatcaccttt ctcaacgaat cccaagatta gtcaaaattt aaactaccgc
    22741 ctctttatac tcggattcac agcacctgcg ggtggcagtt cgccgaccat tgcgatttcc
    22801 ttgagatccg aattatgaaa atgactttgc cgtttaaacc ccatgtgctg gcgctaattt
    22861 gcagtgccgg gctttgtgcc gcctctaccg ggctatatat aaaaagccgc acagtggaag
    22921 cgcctgtgga accgcaatcg acacaacaga ctgcgcctga catcaccgca gttacgcttc
    22981 ctgcaacggt ttccgcaccg cccgtaacgc ccgccgtcgt caaatccgca ttcagcactg
    23041 cacaaatcga tcaatgggtt gcgcctgtcg cgctgtaccc cgattctctg ctttcacaag
    23101 tgttaatggc atcaacctat ccggcaaacg ttgctcaagc agtgcaatgg tcgcacgata
    23161 atccacttaa acaaggcgat gctgctattc aggcagtatc tgaccagccg tgggacgcca
    23221 gcgttaaatc actggtggcc tttccacaat tgatggcatt gatgggcgaa aacccgcaat
    23281 gggtgcaaaa cctgggcgat gcttttctgg cgcagccgca ggacgtgatg gactcggtac
    23341 agcgattgcg gcaactggcg caacaaaccg gctcgctgaa gtcatcaacc gaacagaaag
    23401 ttattaccac aacgaagaaa gctgtaccgg taacacagac agtcacggct cccgtcatac
    23461 catccaatac cgtttcaact gccagcccca tcattacaga gcctgcaaca accgtcattt
    23521 ccattgagcc cgccaatcct gatgtggtct atattcccaa ctacaaccca accgtggttt
    23581 acgggaactg ggccaatact gcgtatccgc cggtttatct gccaccacca gccggagaac
    23641 cgtttattga cagctttgtg cgcggattcg gctatagcat gggtgttgct accacgtacg
    23701 cactattcag cagcatcgac tgggacgacg acgatcatga ccatcatcat catgacgatg
    23761 atgattatca tcaccacgat ggcggtcatc gtgacggtaa tggctggcaa cacaacggcg
    23821 acaacatcaa tatcgacgtc aacaatttca accgtatcac cggtgagcat cttactgata
    23881 agaatatggc atggcggcac aatccaaact accgtaatgg tgtgccctat catgatcagg
    23941 atatggcaaa gcggtttcat caaaccgatg tcaacggcgg aatgagtgcc acgcagctac
    24001 ctgctccaac acgcgacagc cagcgtcagg cggcagcaag tcagtttcag caacgaacac
    24061 acgccgcccc cgtcattaca cgagataccc aacgtcaggc agcggcacag cggtttaatg
    24121 aagctgaaca ctatgggagc tatgacgact tccgcgactt cagccgtcgc caacccctga
    24181 cccagcaaca aaaggacgcc gctcgtcagc gttatcagtc cgcctcgcct gagcagcgcc
    24241 aggcggttcg cgagaaaatg cagactaacc cacagaacca gcagcgaaga gaggcagcgc
    24301 gtgagcgtat tcagtctgca actcccgaac agcgccaggt atttaaggaa aaagtacagc
    24361 agcgcccact gaaccaacag caacgtgata acgcccgcca gcgtgttcaa tcagcatcac
    24421 ctgaacaacg tcaggttttt cgggaaaaag ttcaggagag ccgcccacaa cgtctaaacg
    24481 acagtaacca tactgtcagg ctgaataacg agcaacggtc agcagtacgc gaacgtctct
    24541 ctgagcgcgg agcaaggcga caggaaaggt aaattgcaga cgtaaaaaaa gcggcgtggt
    24601 tagccgcttt tttaattgcc ggatgttccg gcaaacgaac aattacttct tcttcgcttt
    24661 cgggttcggc aggtcggtaa tgctaccttc gaacacttct gccgccaggc ccacagactc
    24721 gtgcagagtc gggtgcgcgt ggatggtcag tgcgatgtct tcagcatcac aacccatttc
    24781 gattgccagg ccgatttcac ccagcagctc gccgccgttg gtaccgacaa tcgcaccacc
    24841 gatcacacgg tgagattctt tgtcgaaaat cagcttggtc ataccgtctg cgcagtcgga
    24901 agcgatagca cgaccagaag cagcccacgg gaaggtggcg gtttcatagc tgatgccttt
    24961 ctctttcgct tctttctcag tcagacctac ccatgcaact tctggttcgg tataggcgat
    25021 ggacgggata actttcggat cgaagtagtg tttcttaccg gcgataactt cagcggcaac
    25081 gtgaccttcg tgaacacctt tgtgtgccag catcggctga ccgacgatat cgccgatagc
    25141 aaagatgtgc ggtacgttgg tacgcagctg tttgtcaacg cggatgaaac cacggtcgtc
    25201 cacttccacg ccagctttgc ctgcgtcgag gtttttaccg ttcggcacac gaccaatcgc
    25261 taccagcacg gcgtcgtaac gctgcggttc agcgggtgct tttttgcctt ccatcgtcac
    25321 ataaataccg tcttctttcg cttcaacggc ggtaactttg gtttccagca tcaggttgaa
    25381 tttcttgctg atacgcttgg tgaagacttt aacgatgtct ttgtcagctg ccgggataac
    25441 ctggtcgaac atttcaacca cgtcaatctg tgaacccagc gcgtggtata cggtgcccat
    25501 ttccagaccg atgataccgc cacccattac cagcaggcgt tctggtactt ctttcagttc
    25561 cagcgcgtca gtggagtccc agatacgcgg atcttcatgc ggaataaacg gcagttggat
    25621 cgggcgagaa cccgctgcaa tgatcgcatt gtcgaagttg atcacggttt tgccgttctc
    25681 accttcaact tccagggtgt tagccccggt gaatttaccc agaccgttga ccactttgac
    25741 tttgcggcct ttcgccatac cagccagacc accggtcagc tgattgatca ctttctcttt
    25801 ccaggtacga atcttgtcaa tatcggtttt cggttcgccg aagacgatac cgtgttcagc
    25861 cagcgctttg gcttcttcga taacttttgc tacgtgcagc agtgctttag aagggataca
    25921 gccgacgttc aggcaaacac cgccaagggt gttgtaacgt tctacgatta cggtttccag
    25981 acctaaatca gcgcaacgga aggcagcgga gtaacctgcg gggcctgccc caagtaccac
    26041 gacctgagtt ttgatttcag tactcatcat gacctctata tatttatctc cggcggtcat
    26101 acccgtcgtc tttcaggccg cagcggcgtt agcttcacgc cctcacccca gtcacttact
    26161 tatgtaagct cctggggatt ctggcgcttg ctaccttgct gcacccagaa atccataggg
    26221 tatgtgaagt aataccctaa ccaccaccgg gtcgttctat ccgtcggtat tttacaaaat
    26281 tgttaacaat ttttaaacaa caaacggcaa ccgatttgtc tattcgctaa taacctcaat
    26341 acattcatga gattaccaga aaaaagccgg ccgttgggcc ggctctttta cttacatcac
    26401 cagacggcga atgtcagaca gcgtgttgtt aatgatggta atgaaacggg caccatcagc
    26461 accgtcgatc acgcggtggt cgaaggagag agaaatcggc agcatcagac gcggcacgaa
    26521 ctctttacca ttccacaccg gctccatcgc ggacttggaa acgccgagga tagccacttc
    26581 cggcgcgttc acaatcggcg cgaagtgggt agtacccagg ccgccgatgc tggagatggt
    26641 gaagcaaccg ccctgcattt cgcccgcagt cagcttaccg tcacgcgctt tcttagaaat
    26701 agtcatcagc tcgcgagaca gctcgatgat gcctttcttg ttgacgtctt tgaataccgg
    26761 aacaaccaga ccgttcgggg tatccaccgc cacaccgatg ttgatgtatt tcttcagggt
    26821 cagacgctga ccgtcttccg acaacgaact gttgaagcga ggcatctgct caagagctgc
    26881 agcaacggct ttcatgatga agacaactgg ggtgatcttc acatccagct tacgtttcgc
    26941 cgcttcttcg ttctgctgtt tacggaacgc ttccagctcg gtgatatcgg ttttgtcgaa
    27001 gtgagtaaca tgcgggatca tcacccagtt acggctcagg ttcgcaccag aaattttctg
    27061 gatacggccc agttccactt cttcgatttc accaaacttg ctgaagtcca ccttcggcca
    27121 cggcagcatg cctgggatac cgccgccagt cgccgccgga gctgcttctg cacgtttgat
    27181 agcttctttc acgtaagcct gaacgtcttc gcgcaggata cgacctttac ggccagtgcc
    27241 cttcactttc gccaggttaa cgccaaactc gcgtgccaga cggcggatca gcggagtcgc
    27301 gtgaacgtaa gcgtcgtttt cagcaaattc agatttgcct tcggctttcg cagccggagc
    27361 tgctgccgga gcttcagctt ttgctgccgg agccggcgct gccgcttcct gtttcgccgg
    27421 agccgccgca ggcgctgcgc cttcaacttc gaagatcata atcagcgagc cagttttcac
    27481 tttatcgcca acgttgactt tcagttcctt cacgacgcct gcaaacggag ccggaacttc
    27541 catagaagct ttgtcgcctt ctacggtaat cagtgactgt tcagcggcaa ctttgtcgcc
    27601 cactttcacc atcacttcgg tcacttcaac ttcgtcaccg ccgatatccg gaacgttaac
    27661 ttctttcacg ccagccgctg gtgcaggggc cgctgccgga gccgcttcct gtttagccgc
    27721 cggagctgcc gcgcctgctt cacccgcgac ttcgaagacc ataatcagcg agccggtaga
    27781 cactttgtca cccacgttca ctttgatctc tttcacggtg ccagcaaacg gagccggaac
    27841 ttccatagaa gctttatcgc cttctacggt gatcagcgac tgttcagctt caactttatc
    27901 gccaactttc accaggattt cggtcacttc aacttcgtcg ctgccgatat ccggaacgtt
    27961 aacgtctttt gccgccgcag ccgcgggtgc tgctgccgga gctgcttctt tcttctcttc
    28021 tgcctgagca ggtgcagcgt ctgctgcacc gtcggcggaa tcgaaaatca taatcagtgc
    28081 gccggtctgg gttttatcgc caacagagac tttgatctct ttaacgatac ccgcctgcgg
    28141 agacggaact tccatagagg ctttgtcgcc ttctacggtg atcagcgact gttcggcttc
    28201 aactttgtcg cccactttga ccaggatctc ggtgatttca acttcatcag ccccgatgtc
    28261 cggtactttg atttcgatag ccattattct tttacctctt acgccagacg cgggttaact
    28321 ttatctgcat cgatgttgaa tttggcgatt gcgtcagcaa ccactttctt atcgatttcg
    28381 ccacgtttag ccagttcgcc cagcgccgca accacgacat aagaagcatc aacttcgaag
    28441 tggtgacgca ggttctcacg gctgtcggaa cgaccgaagc catcagtacc cagtacgcgg
    28501 tagtcgtcag ccggtacgta agtacggacc tgctcagcga acagtttcat atagtcggta
    28561 gatgccactg ccggagcgtc gttcatcacc tgagcgatat acggaacgcg cggagtttcc
    28621 agcggatgaa gcatgttcca gcgttcacaa tcctgaccat cacgcgccag ctcggtgaag
    28681 gaggtcacgc tataaacgtc agaacctacg ccgtaatctt tcgccaggat ctctgccgct
    28741 tcacggacgt gacgcaggat agaaccggag cccagcagct gaactttacc tttgctacct
    28801 tcaatggttt cgagtttgta gataccttta cggatacctt cctcagcacc ttccggcatt
    28861 gccggcatgt ggtagttttc gttcagcgta gtgatgtagt agtaaacgtt ctcttgtttt
    28921 tcaccgtaca tacgctccag accgtcatgc atgatgacag caacttcgta agcgtaagcc
    28981 gggtcgtaag agatacagtt cgggatagtc agcgactgaa tgtggctgtg accatcttcg
    29041 tgctgcagac cttcaccgtt cagggtggta cgaccggaag taccgccgat caggaagcca
    29101 cgcgcttgct ggtcgccagc cgcccagcac agatcgccga tacgctggaa gccgaacatc
    29161 gagtaataga tgtagaacgg gatcatcggc agattgttgg tgctgtaaga ggtcgccgct
    29221 gccagccagg aacaacctgc gcccagctcg ttgatccctt cctgcagaat ctgacctttc
    29281 tcgtcttctt tatagtaagc aacctgctcg cggtcctgcg gggtgtactg ctgaccgttc
    29341 gggctgtaaa taccaatctg acggaacaga ccttccatac cgaaagtacg cgcttcgtcg
    29401 gcgatgatcg gtaccagacg atctttgatc gacttgttct tcagcattac gttcagggca
    29461 cgaacgaaag cgatagtggt agagatctct ttgctctgct cttccagcag cgcgccgaag
    29521 tcttgcaggc tcggcagctc aagcttctcg gtgaagttcg gctgacggct tggcagataa
    29581 ccgtgcagtt tctgacgctg tgcgtgcaga taggtatgct cttcagaacc ttccgggaag
    29641 gtgatgtacg gcagtttttc gatatctgca tcagacaccg gcacattgaa acggtcgcgg
    29701 atgtgacgca cgccgtccat gttcattttc ttaacctggt gcgcgatgtt tttaccttca
    29761 gccgcgtcgc ccatgccgta acctttaatg gtatgagcaa ggattactgt cgctttgcct
    29821 ttggtttcct gcgctttctt gaatgcagcg tagattttct tcggatcgtg accaccacgg
    29881 ttcagtgccc agatctgctc gtcagtccag tctgcaacca gtgctgcggt ttcaggatat
    29941 ttaccgaaga agtgttcacg aacgtacgca ccatctttcg atttgaaggt ctggtagtcg
    30001 ccgtcaacgg tttcgttcat cagctggatc agtttaccgc tggtatcttt acgcagcagt
    30061 tcatcccaac ggctacccca catcactttg atcacgttcc agccagcacc ttcgaagatg
    30121 ccttccagtt cgttgatgat cttgccgtta ccggtgaccg ggccgtcaag acgctgcagg
    30181 ttacagttga taacgaagac caggttatcc agtttttcac gggtagcgat ggtgatcgca
    30241 cctttggatt ccggttcgtc catttcaccg tcgccgagga acgcgtaaac ggtctgttta
    30301 gaggtatctt tcaggccacg gtgttccaga tatttcagga atttagcctg gtaaatagca
    30361 ccaatcggcc ccagacccat agatacggtc gggaactgcc agaattccgg catcagtttc
    30421 gggtgcggat aggaagagag gccattgccg tgaacttcct gacggaagtt atccagctgc
    30481 tcctgagtca gacgaccttc caggaaagca cgagcgtaca cgcccgggga gatgtggccc
    30541 tggaagtaaa ccaggtcgcc gccatcctgc tcgttgcgtg cacggaagaa gtggttaaag
    30601 cacacatcat aaatggttgc ggaagactgg aaggacgcca tatggccgcc cagttcgagg
    30661 tcttttttcg acgcacgcag caccgtcatg atggcgttcc agcggatagc tgaacgaata
    30721 cggcgttcca gttccagatt acccggatac tccggttgtt cttcaacggg gatggtgttg
    30781 atgtagttgc tgatacctgt gcctgcggct acgtttacac cgcctttgcg ggcttcagca
    30841 agcagttggt cgatcagata ctgagcacgc tcaacacctt cttcacggat gaccgattcg
    30901 atcgcctgga gccagtcgcg agtttcgatc ggatccacgt catttgggaa acgttctgac
    30961 atgggttatt ccttatctat ctaataacgt tgagttttct ggaacctgtc ccattgaact
    31021 ctcgccggaa agctcaataa gacaggttct acgtttagtt gccgcgcttt tatatgcgct
    31081 tgatttacaa catcttctgg ataattttta ccagaaaaat cactaattct ttcgttgctc
    31141 cagacgacgc agagaacgct cacggcggct ctcttcacga cttctgtcga gcaaaatttc
    31201 ttcgataaag gccagatggc gatgcgatgc ttcgcgcgct tcttccggct taccggccat
    31261 aatcgcttca aatatgcggg tgcggtgact actcaccagc ggcagcatct cgcgacgcga
    31321 atagagcaat tcgaagttct ggcggacatt ctgggccaac atcggctcca tacaccttag
    31381 cagatgaagc agaaccacat tgtgggccgc ttcggtgacg gcaatctgat actggagtac
    31441 ggcgtttgat tccgcgtcca gatcgccaga ctgctgcgcc agctctatgg cgtggtggag
    31501 ttcacggatg cgttccttgt cttcatcggt actacgcagc gcggcgtaat aagcggcgat
    31561 accttccagg gcgtgtcgtg tttcgagcaa gtcatactgt gactcaggat ggtcggagag
    31621 cagctccacc agcggatcgc tgaagctttg ccataggctg ctctggacaa aagtgccgcc
    31681 accctggcga cgaagcaaca agcccttcgc ttcgagacgt tgaatcgcct cacgcaagga
    31741 gggacgggag acgtcaaact gttttgccag ttcgcgttcc ggtgggagtt tttcgcccgg
    31801 gcggagagtg ccttcgagga tcaaaaactc cagttgctgc tcaatcacat cggagagttt
    31861 tggttggcgg attttgctgt aggccatgag ttcctgtctt aagccacttg ccgaagtcaa
    31921 ttggtcttac caatttcatg tctgtgacgc taaagtaaca aagtattcac cttatgtcca
    31981 tacaggtttt gattgaaatc atgaaactgt gcacatttta acaacttgac atatataacg
    32041 tttcaaagtt gtaactatgc acaaatgtag actttacgta ggaaaggagt tttttaacga
    32101 ttattaaact ataaaaatcc gaattgaacc gattcactta ccaattttgt gatttttaat
    32161 tcaattaaaa cgaatttaaa ttcattctac atattgagag gggttgaggc tgagctttac
    32221 aaacggtttc tttttaagca actcatcttc aaccatgcat aaagcgggtg cattcgctgc
    32281 cgcataccat tattcttgat ctgacggaag tctttttgta acaattcaaa cttctttgat
    32341 gtaaacaaat taatacaaca aacggaattg caaacttaca cacgcatcac tgcgtagatc
    32401 aaaaaaacaa ccaccgcacg aggtttcatg atggaaggtc aacagcacgg cgagcagcta
    32461 aagcgcggcc ttaaaaaccg ccatattcag cttatcgcgc tgggtggcgc gatagggacc
    32521 gggttattcc tgggtagcgc ctccgtaata cagtccgcag ggccagggat tatcctgggt
    32581 tacgccattg ctggttttat cgcctttctg atcatgcgtc agctgggtga aatggtggtc
    32641 gaagaacctg tcgcaggctc ctttagccac tttgcttata aatactgggg cagttttgcc
    32701 ggtttcgcct ctggctggaa ctactgggta ctgtacgttt tagttgccat ggctgagctg
    32761 actgccgtgg gtaaatacat tcagttctgg tatccggaaa tccccacctg ggtttctgcc
    32821 gccatattct ttgtggtgat taacgccatc aacctgacca acgttaaagt gtttggcgag
    32881 atggagttct ggtttgccat tatcaaagtt atcgcggtgg tagcgatgat catcttcggc
    32941 ggctggctgc tattcagtgg caacggcggc ccgcaggcga ccgttagcaa cctgtgggat
    33001 cagggtggtt tcctgccgca cggcttcacc gggctggtga tgatgatggc gattatcatg
    33061 ttctcgttcg gtggtctgga actggtgggg atcaccgcag cagaagctga taacccggag
    33121 caaagtatac cgaaagcaac taaccaggtt atctaccgca tcctgatttt ctatattggt
    33181 tcgttagccg ttctgctctc actgatgccg tggacccgcg ttaccgccga taccagtccg
    33241 tttgtgctga tcttccacga gttaggcgat acctttgtgg cgaatgcgct gaacatcgtg
    33301 gtactgactg cggcgctctc cgtgtacaac agctgcgtat attgcaacag ccgtatgctg
    33361 tttggtctgg cacaacaggg taatgcgcca aaagcgctgg cgtctgtcga taaacgtggt
    33421 gtaccagtaa ataccattct ggtgtctgca ctggtaacgg cgttgtgcgt actgattaac
    33481 taccttgccc cagagtccgc tttcggactg ttaatggcgc tggtggtatc tgcactggta
    33541 atcaactggg cgatgattag cctggcgcat atgaaattcc gtcgcgccaa gcaggaacaa
    33601 ggcgtggtaa ctcgcttccc tgctctgctt tatccgctgg gtaactggat ctgcctgctg
    33661 tttatggcgg cggtactggt gattatgctg atgaccccag gaatggcgat ttcggtatac
    33721 ctgatcccgg tatggctgat cgtgttaggt atcggctatc tgtttaaaga gaaaaccgcc
    33781 aaagccgtaa aagcgcatta atctctctac gccctcaccc gtacagggtg agggcaataa
    33841 tctttacacc aacgccccgt aaatcgtcag tagtgcaatc accaccacga ccacgaacga
    33901 ggttttcttc gccattgaaa ccgctgcctt cggcgtctcc accttatcga catgcggttc
    33961 acgcgccaga gagaactgcg ccagacgcgt taacacctga tactgcgaag tatggaaatc
    34021 acccagcgaa gcaaaccagg ccggtaacgc tttctcacca tgaccgatca aggcatatac
    34081 cacacccgca agacgaaccg gcacccaatc cagtacatga agcacggcat caatgccgga
    34141 ctgtaaacga tgatgcggcg tctgatatcg cgccagccag tattgccatg cacgcaaaaa
    34201 cgcatacccc atcagcgtaa cgggtcccca ggttcccccc acaatcagcc agaacagcgc
    34261 tgcaagataa aaacgaaagt taatccacag caatgcattt tgcagctcac gcaaatactc
    34321 acgttcgtcg cagcctgccg ggacgccgtg aatcatggtg agttcgccag ccatcgtggc
    34381 acgggcatgg ctatcattac gtgaagcagc tgtcagataa gcatgataat gaagacgaac
    34441 tttacctgcg ccaatacaca gcaaaccaat cagcagccac accagtagcg tgggaacgtt
    34501 gaacaatact ccctgcaatg cgcgtaacag taaaaaagtc acgcccatcg caataatggt
    34561 catgcctaac gtgcgcccga gagaaaaatg tttcacccgc cgaaagaacg cttcaagacg
    34621 atgatcaagc tgccagtgct cgcccaactt aaacaggcgc tcgaaaatta acaccagtaa
    34681 ggttgtaaat agcgtcatgt tgtctccttg ctgaccagca cacgaaaccg tgcccaatca
    34741 aatgcaggac cgggatcggt tttccgatcc ggcgcaatat cacaatggcc cgtcatgttt
    34801 ttagcgatat ccggatagca atcaatcagt gcccgcgtaa ccgccgcaag ctgttgatac
    34861 tgcgcatcgg tatacgccag cgtatcggtg ccttcaagct caatcccaat agaaaaatca
    34921 ttgcagcgtt cgcgcccctg atactgagag actcccgcat gccatgcacg tttatcgaaa
    34981 ggaacatact ggactatttc accatcacgg cgaatcaaac agtgagcgga gacgcgcaaa
    35041 tgggcgatct cagcaaagaa aggatgtgcc tgcggatcaa tagttccagt gaataatgcg
    35101 tcgatccacg gaccgccaaa ctcgcctggc ggcaggctaa tattgtgcac caccagcagg
    35161 gtgggtgttt cgtcatccgg gcggcaatcg taatgtggtg agggaacgcg gcgcgcgcca
    35221 accagccacc cctgttctaa caacatgcag gatctcctta tatgtggtgc taatacccgg
    35281 ttcagagtag catgtttcta ccttatgatt cgttagctat ctggagtttt aacatgccgc
    35341 ctcgccgcta taaccctgac acccgacgtg acgagctgct ggaacgcatt aatctcgata
    35401 tccccggcgc ggtagcccag gcgctgcggg aagatttagg cggaacagtc gatgccaaca
    35461 atgatattac ggcaaaactt ttaccggaaa attctcgctc tcatgccacg gtgatcaccc
    35521 gcgagaatgg cgtcttttgc ggcaaacgct gggttgaaga ggtgtttatt caactggcag
    35581 gcgacgatgt caccataatc tggcatgtgg atgacggcga tgtcatcaat gccaatcaat
    35641 ccttgttcga acttgaaggc ccatcccgcg tgctgttaac gggcgaacgc actgcgctta
    35701 attttgtgca aaccctttca ggagttgcca gtaaggtacg ccactatgtc gaattgctgg
    35761 aaggcaccaa cacgcagttg ttggatacgc gcaaaacctt acccggcctg cgttcagctc
    35821 tgaaatacgc ggtactttgc ggcggcggag cgaatcaccg tctggggctt tctgatgcct
    35881 tcctgatcaa agaaaaccat attattgcct ccggctcagt gcgccaggcg gtcgaaaaag
    35941 cgtcctggct gcacccggat gcgccagtag aagtcgaagt agagaatctg gaagaacttg
    36001 atgaagccct gaaagcagga gccgatatca tcatgctgga taacttcgaa acagaacaga
    36061 tgcgcgaagc cgtcaaacgc accaacggca aggcgctact ggaagtgtct ggcaacgtca
    36121 ctgacaaaac actgcgtgaa tttgccgaaa cgggcgtgga ctttatctcc gtcggtgcgc
    36181 taactaaaca cgtacaagca ctcgaccttt caatgcgttt tcgctaatag ttgacagatt
    36241 tatccgctcc atcgcgggcg gataatctcc ccttccccaa ctttcttcgt aacgcctcgc
    36301 aaatttcttg tctttcgctg caacgaaata aaaaacctcg gaatctgctt tcccgtttcg
    36361 ttttttgccc ctcgcctgtg ctgatggcgt ttgccaaagt agcgccaacc aaatcaagga
    36421 gcgaaacaga tggacaagca acgcggtttt acacttatcg aactgatggt ggttattggc
    36481 atcattgcca ttttaagcgc cattggtatt cccgcttatc aaaactacct gcgcaaagcc
    36541 gcactcaccg acatgctaca aacctttgtg ccttaccgta ccgccgtaga gttgtgcgcg
    36601 ctggaacatg gtggattaga tacctgcgac ggtggcagca atggcattcc ctcgcctacc
    36661 accacccact atgtttcagc catgagtgtg gcaaaaggcg tggtgtcgct gaccgggcaa
    36721 gaaagtctca atggactaag cgtcgtcatg acaccgggtt gggataacgc aaacggcgtc
    36781 accggctgga cgcgcaactg caatattcaa agtgacagcg cattgcagca agcctgcgaa
    36841 gatgtcttcc gctttgatga cgccaactaa ggagcggcaa tgaatattcc acagctcacg
    36901 gccctgtgtc tgcgttatca gggagtcttg ctggatgcca gcgaagaagt ggttcatgtt
    36961 gcggtggtcg atgccccctc acatgagttg ctggacgcat tgcatttcgc taccaccaaa
    37021 cgtattgaga tcacctgctg gacgcgccaa caaatggaag gtcacgccag tcgcacacaa
    37081 cagacattgc ccgtagctgt tcaggagaag catcagccca aagcagagtt gctagctcga
    37141 acgttacaat ctgcgctgga acaacgcgcg tctgatattc atatcgaacc agcggacaat
    37201 gcctaccgca tccgcttgcg tatcgacggc gtattgcatc ctttaccgga tgtttcaccg
    37261 gatgccggag tcgcattaac cgccagatta aaagtgctgg gaaacctgga tattgcggaa
    37321 catcgcctgc cgcaggacgg gcaattcact gtcgaactgg caggaaacgc cgtctcattt
    37381 cgtattgcga ccttaccatg tcggggtggt gaaaaggtgg tattaaggtt gttacagcag
    37441 gtgggtcagg cactggatgt caacacgctt ggaatgcagc cgttacaact ggcggacttt
    37501 gctcatgcct tgcaacaacc acagggactg gtgctggtaa ctggccctac cggcagcggc
    37561 aaaacggtca cgctttatag tgccctgcaa acgctgaata ccgctgacat taatatttgt
    37621 agcgtcgaag atccggttga gatccccata gccggactaa accagacgca aatccatccg
    37681 cgtgccgggc tcacctttca gggcgttttg cgtgcgttat tgcgccagga tcctgacgtc
    37741 atcatgatcg gagagatccg cgatggcgaa acagcagaga tcgctattaa agcggcgcaa
    37801 actggtcacc tggtgttgtc taccctacac actaattcca cctgcgaaac gctggtacgt
    37861 ttacagcaaa tgggggtcgc ccgctggatg ctatcatcgg cgcttacgct ggtaatagcc
    37921 cagcgtctgg tacgcaaact ttgcccacat tgtcgccggc agcaagggga gcccatccat
    37981 attccagtca atgtatggcc gtcgccgctg ccccactggc aggcacccgg ttgtgtacat
    38041 tgctaccacg gtttttatgg tcgtacggcc ttatttgaag ttctgcccat aacgccggtc
    38101 attcgtcagc ttatttccgc taataccgac gttgaatcgc tggaaacgca cgcacgacag
    38161 gcgggtatgt gtacgctttt tgaaaacggc tgcctggccg tggagcaagg cttaaccacc
    38221 tttgaagagt taatccgcgt actggggatg ccgcatggcg agtaagcaac tctggcgctg
    38281 gcatggcata accggcgacg gcaatgcgca agatgggatg ctatgggcag agagccgtgc
    38341 tttgctgctc atggcactac agcaacagat ggttacccca cttagcctga agcgaatcgc
    38401 catcaattct gcgcagtggc gaggagataa aagcgcggaa gtcattcatc aactggcgac
    38461 gctactcaaa gccgggttaa cgctttctga agggctggca ctgctggcgg aacagcatcc
    38521 cagtaagcaa tggcaagcgt tgctgcaatc gctggcgcac gatctcgaac agggcattgc
    38581 tttttccaat gccttattac cctggtcaga ggtatttccg ccactctatc aggcgatgat
    38641 ccgcacgggt gaactgaccg gtaagctgga tgaatgctgc tttgaactgg cgcgtcagca
    38701 aaaagcccag cgtcagttga ccgaaaaagt gaaatcagcg ttgcgctatc ccatcatcat
    38761 tttagcgatg gcaatcatgg tggttgtggc aatgctgcat tttgttctgc cggagtttgc
    38821 cgctatctat aagaccttca acaccccact accggcacta acgcagggga tcatgacgct
    38881 ggcagacttt agtggcgaat ggggctggct gctggtgttg ttcggctttc tgctggcgat
    38941 agccaataag ttgctgatgc gccgccctac ctggcttatc gcgcggcaga aattgttgtt
    39001 acgaatcccg attatgggtt cactgatgag gggacaaaaa ctcacgcaga tctttacgat
    39061 tctggcgctg acacaaagtg caggcattac ttttttacag ggcgtagaga gcgtcagaga
    39121 aacaatgcgc tgcccgtact gggtgcaact tctgacacaa atccagcacg atatcagtaa
    39181 cggtcatccc atctggctgg cgctaaaaaa tgccggtgag tttagcccgc tctgtttgca
    39241 attagtgaga acgggagagg catccggctc gctggatctc atgttagaca acctcgccca
    39301 tcatcatcgg gataacacaa tggcgctggc ggataacctc gcagccttac tggaaccggc
    39361 gttgctgatc ataacgggag gaattatcgg tacgctggtg gtggcgatgt atctgccaat
    39421 tttccattta ggcgatgcga tgagtgggat gggataacgc tggcgtgttg ctccacgcca
    39481 gcgttgggag attacaggtt gttgaagatg cggttttctt gttcctgcac ccgaataaac
    39541 gtggtgcgct tggtcagttc tttcaggcgt gaagccccaa cgtatgtaca agctgaacgc
    39601 aagccgccca aaatatcacg cgcggtattt tcaaccgggc ctcgcagcgg cagcttaacg
    39661 gttttacctt ctgctgcgcg atattccgca acgccgccaa cgtgacgttt catcgcagac
    39721 tcggagctca tgccgtagaa cagcataaat ttctcgccgt tctcctcaac gatgcgaccg
    39781 ccgctctctt cgtggcccgc cagcatgccg ccaagcatga cgaaatcggc accgccgcca
    39841 aaggcttttg cgacatcgcc cggcgtggtg cagccaccgt cgctgacgat cattccgccc
    39901 agaccgtgcg ccgcatcggc acattcgatt accgcagaaa gctgcggata accgacgcct
    39961 gttttgacgc gagttgtaca aacagaacct gggccaatgc caactttaac gatatcagca
    40021 ccggagagga taagctcctc acacatttca ccagtcacta cgttgccagc acaaatggtt
    40081 ttggtcggcc acgcttcacg cgctttcgca acgaactgca cgaagtgttc ggaataacca
    40141 ttcgccacat caatacaaac gaagtttaat gccgggttca ggtcgagaat ctgtttagtt
    40201 ttttcgaaat ccgcatcaga cgtaccggta gaaaccatca catgtttcag cacatcagcg
    40261 gaagaattgt tgataaacgc ttgccactct tcgacagaat agtgtttatg cacagcagtc
    40321 aaaatatcaa aagaagccag cgcagaggcc atagaaaatg tacctacggt gtccatattt
    40381 gcggcgataa ttggcacgcc ggaccagctc tgacctgaat gtttgaaggt gaattgacgt
    40441 tccagttcaa catcggaacg gcttttaaga gtggagcgtt tagggcggat gagaacgtct
    40501 ttaaaaccta acttcagatc ttcttcaata cgcatgtgcg gattcctggg gttaatggct
    40561 ataagggtaa gagcgcaact ccagtgacgc tatcatacgc actaatcaat gcggcgcaag
    40621 actgcgaaat tgtctttttt tacgctacaa tcccataaat ttacctggta aatagtgagc
    40681 gaacgtttca tcaatttcgc tttcgtgcac tcatttttaa cgttttgata atcatgatga
    40741 attcccggga atataaatta tgaggtatat agttgcctta acgggaggca ttggcagtgg
    40801 caagagtacc gttgccaatg cgtttgctga tctcggaatt aacgtcattg atgccgatat
    40861 tattgcgcgt caggtggttg aaccaggtgc acctgcgcta catgccattg ctgatcactt
    40921 tggcgctaac atgattgctg ctgatggaac attgcagcgc cgggccttgc gcgagcggat
    40981 cttcgccaac ccggaagaga aaaactggct taacgccctg ctgcatccgc tgattcagca
    41041 agagacgcaa caccagatcc agcaagctac ttccccctat gtactgtggg ttgtgccatt
    41101 gctggtagaa aactcactgt ataaaaaagc gaatcgagtg ctggtggtgg atgtcagccc
    41161 agaaacgcaa cttaagcgca ccatgcagcg cgatgatgta actcgcgagc atgtcgaaca
    41221 aatccttgct gctcaggcaa cgcgcgaagc ccgccttgcc gtggcagatg acgtcattga
    41281 taataacggc gcaccggatg ctatcgcatc ggatgttgcc cgcctgcacg cacactattt
    41341 gcagcttgcg tcgcagtttg tctcacagga aaaaccgtaa tgcagaccca ggtccttttt
    41401 gaacatccac taaatgaaaa aatgcgtaca tggctgcgca ttgagttttt gattcagcaa
    41461 ctcaccgtta atttacccat cgttgaccac gctggcgcgc tgcatttctt ccgtaatgtc
    41521 agtgaattac tggatgtttt cgagcgcggc gaagtccgca ctgagctgtt gaaagaactt
    41581 gaccggcagc aacgtaaact ccagacctgg attggcgtgc ctggcgtgga ccagagccgt
    41641 attgaagcat taattcagca gttaaaagcg gcggggagcg tattaatttc cgcgccgcgt
    41701 atcgggcaat ttctgcgtga agatcgtttg attgctctgg tgcgtcagcg actgagcatc
    41761 ccaggcggct gttgcagctt tgatttacct acattgcaca tttggctgca tctaccccag
    41821 gcgcagcgcg acagccaggt agaaacctgg attgccagcc tgaacccgct cacccaggca
    41881 cttaccatgg tgctggattt aattcgccag tcggctccct tccgtaaaca aaccagcctg
    41941 aatggttttt atcaggataa cggtggcgat gccgacttgc tgcgcctgaa tctgtcgctc
    42001 gattcacagc tttatccgca aatttccgga cataagagcc gttttgccat tcgttttatg
    42061 ccgctggaca ctgaaaacgg acaggtaccg gaacgtctgg atttcgaact ggcctgttgc
    42121 taaggagtga aaatgtcaga aactattacg gtgaattgcc caacctgcgg gaaaacggtg
    42181 gtgtggggtg aaatcagccc gtttcggcca ttttgctcca aacgttgtca gctgatcgac
    42241 ctcggagaat gggctgctga agaaaaacga atccccagca gtggcgatct ttccgaaagc
    42301 gatgactgga gcgaagagcc aaagcagtga catttgtctg atgccgcacg taggcctgat
    42361 aagacgcgga cagcgtcgca tcaggcatct tgtgcaaatg tcggatgcgg cgtgaacgcc
    42421 ttatccgacc tacagacgct taagcttcgc aattaccggt tcattggctg gcggaaaatc
    42481 atcggcatta agaccgacta gcgacatcca ctcaccgggt tgcccttctt taccccacgg
    42541 ctccccttcc cagctttcga ccagccagaa ccacagagtt atatgcctgt ccgggaattc
    42601 atattccagt ttttcaaata gcgaaaaatg ttgtggggta atcccgactt cttcctgaag
    42661 ttcacgcacc accgcctgtt ccggcgtttc gcccatttca attttaccgc cgggaaactc
    42721 cagtttattc gccatgtgcg catctgctgc gcgacgcgtt ataaagattt cattgttctc
    42781 gttgcgaata atacctaccg caatttgcag ctttttcatt gtcttaaacc tataaaaaag
    42841 gcgcagaatc ctgcgccttt tacttcaact gttagctttt attgcaggcg accatggcac
    42901 tgcttgtatt ttttaccaga accgcacggg caaggatcgt tacgtcctac tttgcgctca
    42961 ccggtttgcg ccgccagtgc agctgcggct gcagagtcgt catcctgatg gctaagctgc
    43021 tgcatttgcg ctaaacgctc ggcttccata cgacgctgtt gttccagctc ctcaacctct
    43081 tcaggcatac gtacctggac tttgctcagc gtgctgataa cttcatattt taacgactcc
    43141 agcatcgctg caaacatgga gaacgattca cgtttgtatt cctgcttcgg atctttctgt
    43201 gcatagccac gcaggtggat accctgacgc agatagtcca tcgctgccag gtgctctttc
    43261 cacagggagt caagcgtttg cagcatgacg cctttctcga agtgacgcat catctcagca
    43321 ccaaccactt cttctttacg ctgatacact tcgatggact gcgccagaat gcgctcacgc
    43381 agcgtctctt catgcagttc tggttcttta tccagccact cggcaattgg taaatcgagg
    43441 tcgaaatcgt tcttcagacg ttcctgcagc cccggaatat cccacatttc ttccagcgac
    43501 tgtggcggaa tgtaggcatc aatggtggct ttgaacacat cttcacgaat gctgttgatg
    43561 gtttcgctca catcgctgac atccagcagt tcgttacgct gggagtaaat ggcgcgacgc
    43621 tgatcgttag ccacgtcatc atattccagc agttgcttac gaatgtcgaa gttacggctt
    43681 tcaactttac gctgggcgtt ggcaatcgct ttagtcaccc acgggtgttc aatggcttcg
    43741 cctggcttca tacccagttt acgcatcatg ccggatactc ggtcggaagc aaaaatacgc
    43801 atcagcgcat cttccatcga caggtagaaa cgggaagaac cagcatcccc ctgacgacca
    43861 gaacgaccgc gcaactggtt atcgatacga cgggattcgt gacgctcggt accgatgata
    43921 tgcaggccac ctgcttccag taccgcatcg tgacgtacct gccagtcggc tttaattttt
    43981 tcaatttgct ctgcggtcgg attttccagc gcggcaactt ctgcctgcca gctaccaccg
    44041 agcacaatat ctgtaccacg acccgccata ttggtcgcga tagtcaccgc agccggataa
    44101 cctgcctgag caacaatcgc cgcttcgttg gcgtggaatt tggcgttcag gacgttgtgc
    44161 ttaataccgg ctttggtcag ttcgtttgac accagctccg atttttcgat ggagatagta
    44221 cccaccagca ccggctggcc tttcgcagta cgttctttga tatcttcaat gatcgcctga
    44281 attttttccg cttcagtcat gtagaccagg tccggcagat ctttacgaat cattggacgg
    44341 ttggtcggaa caacgacggt atccagcttg tagatggagc taaattcgaa agcttcggta
    44401 tcagcagtac cggtcatccc cgccagtttt tcatacagac ggaagtagtt ctggaaggtg
    44461 atcgaagcca gcgtttggtt ttcgttctgg atctgcacac cttctttcgc ttccacagcc
    44521 tggtgcagac catcggacca gcgacggccc tgcatggtac gaccggtgtg ttcgtcaacg
    44581 atgataactt caccatcttt aacgatgtag tcgacgtcgc gggtaaacag cgcatgagcg
    44641 cgcagcgccg ccgttacgtg gtgcatcagc atgatgttgg ccggagagta cagagactcc
    44701 ccttcatcca tgatgccctc tttcaccagc agttcttcaa tcagcaccag accacgttcg
    44761 gtcaggttca cctggcgaga tttttcgtcc accgagaagt ggccttcgcc ctggaaggtt
    44821 tcggagtctt ctttttcctg acggatcagg tgcggaataa ttttattcac gcgtttatac
    44881 atttccgagc tgtcttctgc cgggccggaa atgatcagcg gtgtacgcgc ttcatcgatc
    44941 aggatggagt ccacttcgtc caccagcgca tagtgcagtt tacgctgtac acgttcttca
    45001 gggctgaacg ccatattgtc gcgcaggtag tcaaagccgt attcgttgtt cgtaccgtaa
    45061 gtgatgtcag ctgcgtaagc ttcgcgcttt gccggtgctg gcatgcccgg caggttgata
    45121 ccgacagtca ggccaaggaa ttcaaacagc ggacggttgt tttcggcgtc acgttgcgcc
    45181 aggtagtcgt tgacggtaac tacgtgcacg cctttaccgg ttagtgcgtt caggtaagca
    45241 ggcagcgttg cggtcagggt tttaccttca ccggtacgca tttcggcgat gcagcgttcg
    45301 ttaagaacca taccgccgag taactgaacg tcgaagtgac gcataccaaa gacgcgctta
    45361 cttgcctcac gtaccacggc gaaagcttcc gggatcagat tttccagcac ttcgcctttt
    45421 tccagacgtg cacgaaactc tgcggttttc cctttcagtt cttcgtcgga gagtttttcc
    45481 atctccggtt ccatggcatt gatgatgttg accactttgc gcatccggcg cagggtgcga
    45541 tcgttacgac taccgaaaac tttagttaac aatttgatta gcataataaa atctcaaacg
    45601 ccccgcgttg cggagttaat aaaatgaagt aaaggtttat tgttgttagg tgaggcgttg
    45661 agggccagca cggatgcctt gcgcctggct tatccagacg ggcgtgctga attttgcttg
    45721 tggggtaaaa tgcgcataat caatgcgata acccttttca gacggcgtgc cttcctgggt
    45781 cagcagcgcg ctgagcgtat ccagtaatgc aagatgttgc gcctgaagag gcaaagattc
    45841 ttcagcaacg ggcagtgttt gcggtgccat tgcgaaagaa agatgacgga ttaccgtgcg
    45901 aatggcatgt tgatgccagt aatcaacgga atagttcgaa ttcgggcggc gtgtgttcgc
    45961 ttccagcaag gccaattgac caaagttaac tttggctgaa ggctcgtggt tgcgggttgt
    46021 cgcttttgcg ggcgcgtttg gttcggcggc gttgctgagc gcaggcaaac ctaaactcgc
    46081 cgcaaccatc cctaataaga gatgcggcca gaagtagcgt ttaccaaact gtcgccagcg
    46141 cgtcagtatt ccactcacgt tattgccatc ccgatcatat tcaaacgtat ttagcgcaca
    46201 aatcttatca ttaaagcctg cagtccacag cacgaatgaa gataagttgt gcggaaaagt
    46261 gcttaagaaa acagcgttcg caccgttttt tatagctctt ataaaattca aaaaaaaacg
    46321 actggttcac ctggccggag agagtgccag atttgccagt cgaattttat acgacagtat
    46381 aaatgtcgtt atgccagtac agctgaaggc gctttgaagg ccaacggcag ttctgcgtcg
    46441 tcctggaagg tcacatattc ccaggcttcc tgtttcgcca ggacagcctg cagcagtttg
    46501 ttattcagtg catgaccgga tttataagcg gtaaatgcac caataatatt gtgaccacac
    46561 atgaacaagt caccgatcgc atcgagcatt ttgtgacgca caaattcgtc ttcaaaacgc
    46621 aggccgtctt cgttcagtac gcgataatcg tcaacaacga tggcacaatc gaagctgccg
    46681 cccaggcaca aaccacggga ctgcagatat tcgatatcac gcatgaaacc gaacgtacgc
    46741 gcacggctga tctggcgcat aaacgcatca gcggagaagt tcatcgcata gcgctggttg
    46801 ctggaatcaa tagccggatg gttaaaatcg atggtgaaat ccagcgaaaa accattgtac
    46861 ggcttaaatt cagcccactt atcgccatct tcgacacgaa cagtctcttt gatgcgaaca
    46921 aattttttgg cgcagttcaa ctcgtcgata ccggcgtcaa gcagcaggta tacaaacgga
    46981 gcggcgctgc cgtccatgat cgggatttcc ggcgcgttaa cttcgataac aatgttatcg
    47041 atgcccaagc ccgcgagagc agcattgagg tgctctacgg ttgaaatccg tacatcatgc
    47101 tcgttgacca gacacgtaca gagcatggta tcacgcacag atttggcatc ggccgggaaa
    47161 tctaccggtg gattcaagtc ggtgcgacga tagatgaccc cggtgttggc cggcgcaggg
    47221 cgtaacgtca gggtgacttt cttgccggta tgtaaaccga cacccgtcgc ctgaacgata
    47281 cgtttaagtg tcctttgttt gatcatcgta ttatctcgcc aaattaccta tccaaccgaa
    47341 gtgtactata cattcggcgg gccagtttag cacaaagagc ctcgaaaccc aaattccagt
    47401 caattcttaa tcagcttgct tacgcaggaa tgctgggata tccagataat ccggctcttt
    47461 cgcagtttgc ggcgcattgt cattcacgac tttagcaacc ggcttctgct cctgggtcag
    47521 cggagccatc ccatgctgct ggtagcgatc catcactggc tgctgaacct gcttattggt
    47581 caccagagtg atttcaggac gtttgtccat gccgatacct gtcgcaacaa cggttacgcg
    47641 cagctcgtca ttcatatccg ggtcaagaga agtaccgata accacagtcg cgttgtcgga
    47701 agcaaatgca cggatggtgt tacctaccgt ttcgaactca tccagacgca ggtcgaagcc
    47761 cgccgtgatg ttaaccagca cgccgcgcgc gccagacagg tcgatatctt ccagcagcgg
    47821 agaagagata gccatttcag cagcttcttc cgcacggtct tcaccgctcg ccacgccaga
    47881 acccatcatt gcgtagccca tctcagacat tacggtgcgt acgtctgcaa agtccacgtt
    47941 catcaaaccc ggacgagtaa tcagttcagc gataccttgc acagcgcctt tcagtacatc
    48001 gttcgctgcg ccaaacgcat ccagcaggga gataccgcgg cccagaactt tcagcagttt
    48061 gtcgttcggg atagtgatca gagagtccac atgcttggac agttcagtga tcccctgctc
    48121 cgcgaatgcc atacgcttct tgccttcaaa gttgaaaggc ttagtgacga cagcaacggt
    48181 caggataccc aaatcttttg ccacttcagc gacgactggt gctgcacctg taccggtacc
    48241 accacccata cccgcagcaa taaagaccat gtctgcacct tccagcgccg cacgcaatgc
    48301 atcgcgatcc tcatcagccg cattgcggcc aacttctgga ttagcgccag cgcccagtcc
    48361 tttggtgata ccgctaccga tttgaatcgt ctgtccaacc gctgttttac gcagcgcttg
    48421 tgcatcggta tttaccgcga agaattcaac accttcaatg cgctcgcgca ccatgtgttc
    48481 aacagcatta ccgccgccgc cgccgacgcc gatgacttta atcaccgcgt cattggtaag
    48541 ttccattggt tcaaacatag tttctctccg atttgtgcct gtcgcctgag gccgtaatca
    48601 tcgtcggcct cataaaaatt aaaactcttt tcgcagccaa ctattgagtc gcttgatcca
    48661 cgagccaact gatgctgtaa cacgtttttc tacttcagct tcaccgttaa gatgtgactc
    48721 tttcccatag tgaagcaatc ccaccgccgt cgaataatac ggctcctgag cataatccgt
    48781 taaaccggta atgttcagcg gcgcgccgat acgcacttgc gtatgaaaca cgcgctgagc
    48841 acaggctgca agaccttcga tctgcgctgc gccaccggtt aatacaatgc ctgccgccag
    48901 gtgatgttta accccttgtt ggcgaagctt ttcctgcaac tgcaatatct cttcgttgac
    48961 caggttgagc agctcggtat agcgcggctc gatcacctct gccagggtct gacgttgcag
    49021 actccgcggc ggacgaccgc ctacgctcgg cacttccacg ctctcatctt ttccaacgat
    49081 ggaacccagc gcacaaccgt ggcgaacttt aatcgcttcg gcgtcgcttg gcggcgtgcc
    49141 aaaggcgtaa gcgatatcac tggtcacgac attgccagca taaggaatta ccttagtgtg
    49201 gcgcaatgcc ccaccggtat aaacggcgat atccattgta ccaccaccga tatcgacgac
    49261 gcagacaccc agttcacgtt catcttccgt caataccgaa taacttgatg ccagtccggc
    49321 aaatatcagt tggtcaactt tcagtccaca acgttcaacc gctttgacga tgtttttcgc
    49381 catatcgttg tgacatgtga tcaggtgcac ttttgcctgc atcctcacgc ccgaaagtcc
    49441 taccggattc ttgatccctt cctgatagtc aatcgcatac tcttgcggga tcacatgcag
    49501 cacacgatgc tcatcgcgca cacgcaccga tttcgcggta tggacgacgt tttccacatc
    49561 ttcttgcgtc acttcttctt cagaaatagg caccatacca atttcattct ggcagctgat
    49621 gtgcttacca gaaagcgcca gatataccga agagatctga caatctgcca tcaattctgc
    49681 ctggtcaatg gcacgttgta cgcacttgac cacggattcg aggtcgttca ccccgccttt
    49741 atccatacca cgcgacgggc agctgcccac gccaatgata ttgaccatac cgtcgggcag
    49801 aacttcccct actaaagcgg caaccttcgc ggtaccaatc tccagtccta ctaccagttt
    49861 tctgtccgtc gccttgatca ttgttgttct gcctgtgcct gattttgttg ctgagtagat
    49921 tcctctggcg gcaagggcgc ccagcctact gccgctccag agtcataacg caaatcaacg
    49981 tagctaatcc gtttgccatc ggtttgcgcc tgctgctgta aaaccggata aagttctaca
    50041 aagcgagcca aacgtttcat cgtatcgccc cggccaagat tgagcttaat atcgttattc
    50101 agcgtcaact gccaggaacg ccgcgcggtc atcgccgctt ccttcagagt aaatctgtcc
    50161 tttgccagca tctgccccat ttcgcgatag ccctgcaaca cttcattggc gctgccttcc
    50221 gggccataca gcattggaag cacctgcttg ctggtgcgat ctggcggcac gctgaaggtg
    50281 tttccttccg cgtctaccat atgttgatca ttccaccgcg caatcggcac atattcaacc
    50341 agatgaatct tcaattcatc aggccactgc tttctgacgc tcacctgctt aatccacggc
    50401 aggcgttgtt ctatttgcgt ctgaatgatg ttgacatcct gggtcataaa ggtacccggc
    50461 tcacccaatg ccaggatcga ctgccggata tcgtcattac gcgtgtaatg gcgttcaccg
    50521 gtcaacacca gctttgagag cggcaggcgt tgcgcatctt ccatccagcc caacacgacc
    50581 cagccgctca ccaacactgt cgttaaaacg gtcagcagga aaaggatccc cgccagacgc
    50641 gttccattat tgcggcgaga agaaacctct tcttcactgt ttcgcgtgtt cagagcagcc
    50701 tgcgacatat tagtccgcca gttccagaat tcgtactacc aactgcgaga agctcatacc
    50761 tgcctgacgt gccgccatcg gcaccaggct gtggctggtc atacccggtg aggtattggc
    50821 ttccagcaga taaaactgtc catcgctgtc cagcataacg tcaatacgtc cccatccttt
    50881 gcaacctaac gtcgtccatg ctttcagcac taatgcctgc aaattggcct cttgtgacgc
    50941 ttccagacct gcggggcaga aatactgtgt ctcatcagag agatacttcg cctcataatc
    51001 atagaaggtt ccggacggtt gaatacgtat tgacggtaaa atttcttcac cgagtatcgc
    51061 aaccgtgaac tccggcccac ttagccattt ttcaatcaat acttcttcat cgtgctgaaa
    51121 tgccaatctt aatgcatctt gtagagcatt ttctgctact acttttgaca ttcccacact
    51181 ggaaccttcg cggctcggct taacgataac cggcaaaccc agagcagaaa tttctgctaa
    51241 ctgcttatcg ctcaggcctt tttcaaactc tgcgcgggtt aacgctaccc acggcgcgac
    51301 cggtaaaccg gcaccttgcc acagcagttt gctgcgtagt ttatccattg aaagcgcaga
    51361 tgccatcact ccgctgccgg tataaggcaa gcccatcagc tcgagcatgc cctgcagcgt
    51421 accatcttca ccgccgcgac cgtgtagcgc gataaacact ttctgaaaac ccatcgactt
    51481 cagttgcgtc acgtcgactt ctttcgggtc gacaggatac gcatcaatac cgccttcacg
    51541 cagcccggct aacaccgctg cgccagaatt cagagaaact tcccgctcag cggaagtccc
    51601 gcccaacagg accgcgattt tatcagtcat gttgttcttc ctccggagtt tgcggcttca
    51661 gtttgatttc agctaaagaa cgggcaattt ttccgatatt accagccccc tgaacgagaa
    51721 tcaggtcgtt accggttaat accggtgcca gcatctcggc tacctgcgca gggtccggca
    51781 ccagaatggg atcaattttc ccacgtccac gaattgtgcg acacagcgaa cggctgtccg
    51841 ctcccggaat tggcgcttcg ccagccggat acacttccag catcaacagg gtatcaacct
    51901 gcgtcagcac attggcgaaa tcatcataca ggtcgcgcgt acgggtaaaa cggtgcggct
    51961 gaaacagcat taccaggttt ttatccggcc agcctgcgcg cgccgcttta atggtggcgt
    52021 caacttccgt cgggtggtgg ccgtagtcat cgaccagcat tgccgtaccg cttttaccat
    52081 tcactggctc cagcgggaat tcaccgagga aatcaaaacg gcgcccagtc ccctggaagc
    52141 tctccagcgc acgtaaaata gcctcgtcat caattccctc ttccgtagca accgcaaccg
    52201 cagctgcggc gttcagcgcg ttatgacgac ctggcgcatt cagggtgacg cgcatcggct
    52261 ctttgtcctg gcgcagcagc gtaaagtgcc cctgcgggcc aatctgctga taatcttcta
    52321 cacgcacgtc ggcatcttcg ctgaagccgt aagtcgtggt ctgacgtccc actcgcggta
    52381 acaattcgcg gatcaccgga tcatcaacac acatcaccgc acgaccgtaa aacggcaggt
    52441 tgtgcagaaa attaataaaa gtctgtttta aattctcaaa gtcgccctgg taggtatcca
    52501 tgtggtcggc ttcgatattg gtgacaatcg ccaccatcgg ttgcagatgc aggaacgatg
    52561 catcactctc atctgcttcg gcaatcaggt accgaccatg ccccaaacgc gcatgaaccc
    52621 ccgccgcttt taccagcccg ccgttaacga aggttgggtc gagccccgct tctgcgtaga
    52681 tgctggaaac catcgcggtg gttgtcgttt tgccgtgcgt tcctgcaatg gcgatgccat
    52741 gacgaaaacg cattaactca gccagcattt cggcacgacg gatcaccgga atacgcgctt
    52801 catgagctgc gacaatttcc gggttatcgg cagaaatggc gctggaaaca acgaccacgc
    52861 tggcatcacg tacgttttcc gggcgatggt tgaaataaat cgtcgctccc agattcatta
    52921 actgctgcgt gaccggattt ggcgctaaat cggaaccact gatctgataa ccttcattgg
    52981 ccagaacttc ggcaataccg cccataccgg caccaccgat gccgacaaaa tgtatgtgcc
    53041 gaacgcgacg catttcgggc acgatggaac gcagttttgc caattgttgt gtattcattc
    53101 tttacgccat taacttctta aattcatacg atgcaaaagg catcgctaca attacgcccg
    53161 ggcagcccgg ctcacttcat ttgccactcg ctcggtggca tccggaatgg atgcagcgcg
    53221 ggcgcgttct gccatggtta ataaggtttc tcgcgaccac ccggccaggg tgttggcgac
    53281 agcatccacg ctaagctgtg gctgctcgat aattttggct gcgcccgctt tttccagcgg
    53341 tagcgcattc cagtattgct ggcggtcttt atgttgaaac ggcacaaaca acgccggtag
    53401 tcctgccgcg gcgatttcac tcaccgttaa agcaccggag cggcaaacga cgacatccgc
    53461 ccacgcatac gccgccgcca tatcatcaat aaattccgtc actttatgct gcggttgccc
    53521 cgcttcggca tacgcctgtt caacggattg ttgcgaacct ttaccgctct gatgccagat
    53581 agtgactgaa tcacccagtt tcgcagcaac ctgcggcatt gtctggttaa gaatgcgtgc
    53641 gccctgggaa ccacccacta ccagcacacg aaccggacct tcacgtccag ccaaacgttg
    53701 ctgcggcaac ggcagcgcca gtacatcggt acgcaccgga ttacccacca cttccgcatt
    53761 agggaaagcg cctggaaacg cctgcatcac tttggtggca atcttcgcca gccatttatt
    53821 ggttaagccc gcaataccgt tttgttcatg aagtacaacc ggaatgccta acgaccacgc
    53881 ggccagacca cctggacctg atacatagcc gcccataccg agtaccacgt caggtttgta
    53941 cgctttcata atcgcccgcg cctgacgcca ggcgttgaag atacgcagcg gcgcagctat
    54001 cagtgctttt ataccttttc cacgcagacc agagatacga atgaaatcaa tttcgatgcc
    54061 atgttttggc actaagtccg cttccatacg gtcggcagtc cccagccagc gaacttgcca
    54121 accctgagcc attagatggt gcgcaaccgc cagtcccggg aatacatgtc caccggttcc
    54181 gcctgccatc accattaatc gctttccttg accactcatc gtgaacctcg tacaaacgcc
    54241 tgcgctttct ccagacgcgt ttcataatca atacgcaaca gcatcatgat ggctgtcgac
    54301 ataatcagta agctcgaacc accgtaactg atcagcggca atgtcagacc tttggtcggt
    54361 aacatccccg ccgccgcgcc tacgttaacc agcgcctgga agctaaacca gatgccaata
    54421 gaacaggcga gaaaaccgga aaaacggtgg tcaatttcta acgctttgcg gccaatcgac
    54481 atcgcgcgaa aagcgacgaa gaataccatt aaaagtgcca gcaccacacc gacatacccc
    54541 agttcttcgc cgataatggc gaaaataaag tcagtgtgcg cttccggcag atactccagt
    54601 ttttgtaccg agttacctaa accttgtccc caaagttcgc cgcgaccaaa cgccatcagc
    54661 gattgcgtta actgatagcc gctgccaaag ggatcttccc acgggttcca gaatgcggta
    54721 acacggcgga tacggtacgg ttcggcgagt atcagcaaca caaccgctga aatgcccata
    54781 ccgataatgg caatgaactg ccacaatttc gctcccgcca ggaacaacat cgccagcgta
    54841 gtcacaaaca acaccaccac cgtaccaagg tctggctgtg ccagcagtaa cactgccaac
    54901 accagaatca cgcccatcgg tttcaggaag ccgcgcaggt tattacgtac ttcgtcgcct
    54961 ttacgcacca gatagttggc gatataacaa aacagcgaca gttttgtcag ctccgcaggc
    55021 tggatacgca gcaaaccgag atcgatccaa cgcgatgccc ctttaaccga gctacccact
    55081 accagcacga tcatcagcag gatgatagat ccgagcagca tcgtggcact gtagcgttgc
    55141 cagaactcca tcggcagacg cagcgtaatg atcgccagaa tgaatgccag gatcagatag
    55201 acgccatcac gcttcgcgaa gaagaacgga tcgttggtta agcgttgccc tatgggcatt
    55261 gacgccgagg tcaccatgat aaagccaatc gccgcaaggc cgaaggtcag ccacagtaag
    55321 gtgcgatcgt acatgatcag gctgtcggta tctttttccc gcgagcccat cacccagcct
    55381 tttagcgccg tggagatcca gaccaggata ctgaatcctg gcaggcgcgg cattttcagg
    55441 cgagggagag ataaacgcat caacctaact ccttcgccag acgggcaaac tcattgcctc
    55501 gttgttcaaa gttcttgaac tgatcaaggc tggcacaggc cggggagagc agaaccatat
    55561 cgcccggctg aacacgcgga gccagcaagc gcatcgcctg ttccatagtt tcggtttgtt
    55621 ctgccacttc cgggcgtagc gccgccagct gcgcaccgtc acgaccaaaa cagtacaaac
    55681 gcacgttgtc gccattcaga tagcgcgcca gtggactgaa gtccgccgat ttaccatcgc
    55741 cgcccagcaa caaatgcagc gtgccgtcta cctgcaagcc attcaacgcc gcttccgtac
    55801 tgccgacgtt agtggctttc gaatcgttaa tccagcgtac gccattgtgc tccagcacca
    55861 cttcaaagcg atgcggcaga ccggcgaagg tggttaacgc tttcaggctg ctggcgcgcg
    55921 gcaaaccagc tgcgtctgcc agtgccagtg ctgccagcgc attggtgtag ttatgctgcc
    55981 cggaaagttt catctctttc acgttcagca ctttctcgcc tttcactcgc agccaggttt
    56041 ctccctgctg atgattcagg tgatagtcac ccatgttgac gccaaagctg acgcagcgtt
    56101 catcagcacc gcgaatcggc attgttaagg catcatcagc attgacgaca caaactttcg
    56161 cattttcgta aatacgcaat tttgccgcac ggtattgctg caaaccaaac ggatagcggt
    56221 ccatatgatc ttcagtcacg ttcagaatgg tcgccgccac cgcctgtaag ctggaagtgg
    56281 tttccagctg gaagctcgac agttccagca cgtacagttc gcactcagca tcaagcagca
    56341 tcaacgctgg caggccaata ttgccaccca caccaacgtt aacccccgcc gctttcgcca
    56401 tttcacccac tagcgtggtg accgtgcttt tgccgttaga accggtaatc gccacaatcg
    56461 gtgcttgtgc ttcgcgacag aacagctcga tatcgccaac gatttcgatt ccggcatcag
    56521 cggcagcgct taaggatgga tgcgccagtg caataccggg actggcgaca atcagatctg
    56581 ccgccatcag ccattcatca ttcagactgc ccgtgtggcg ttctacggct tcgggtaatt
    56641 tatccaggcc aggcggtgtc atacgcgtat ccataacgcg cggcgtcaca ccgcgagcga
    56701 ggaaaaagtc cacgcaggaa agcccggtga ggcccaggcc gataatgacg acatttttac
    56761 cctgataatc agccatgatt aacgtacctt cagcgttgcc agaccaatca gaaccagcat
    56821 cagcgaaata atccagaaac gcacaatgac gcgcggttcc ggccagcctt tcagttcata
    56881 gtggtgatga atcggtgcca tgcggaaaat acgttgtccg cgcagtttaa aggagccgac
    56941 ctgcaggatg acagaaagcg tttctaccac gaacacgccc cccataatca ccagcaggaa
    57001 ttcctgacgt agcagtacgg cgataatgcc taacgcacca cctaacgcca gcgaacctac
    57061 atcgcccata aagacctgcg ccggataggt gttaaaccac aggaagccca gtcctgcccc
    57121 gactatcgcc gtgcagacaa tgaccagttc cccggcgtgt cgcagatacg gtatgtgtaa
    57181 atagctggca aagttcatgt tgccggtcgc ccacgccacc agcgcaaaac caccggcgac
    57241 aaatacggtc ggcataattg ccaggccgtc gagaccatcg gtcaggttta ccgcgttgcc
    57301 agtacccaca atgacgaagt aagccagcag aatgtagaac agccccagtt gcggcatcac
    57361 atctttaaag aatgggacca ccagctgcgt tgcgggcgtg tctttgccgg caaggtacag
    57421 ggcgaaggcg acacccagcg caatgaccga catccagaaa tacttccaac gagcgatcaa
    57481 ccctttggtg tctttacgca ccactttgcg ataatcatca acaaagccaa taacaccgta
    57541 acctaccagc accaccaaca cgcaccagac gtacggattg gacgggtaag cccacagcag
    57601 tacggagatc actatcgccg tcaggatcat aatcccgccc atggtcggcg taccgcgctt
    57661 gctgaagtgt gattcaggac cgtcgttacg caccacctga ccaaaggaaa gtttttgcaa
    57721 atgagcaatc atacgcgggc ccatccacaa tgagatgaac agcgcggtca gcaggctgac
    57781 gatggcgcga aacgtcagat aggaaaagac gttaaagccg gaataatatt tgaccaaatg
    57841 ttcggccagc caaactaaca tgtcccattc tcctgtaaag cgcgtactac ctcttccatg
    57901 gcggcactac gtgaaccctt aactaaaatc gtaattacct gttgctcagc aatcagtgat
    57961 ttaagacgcg taattaacgc agttttatca gcaaaatgtt cgccaacgcc gctggcggtg
    58021 ctgatagcat ggctttgttt acccacgctt aacacgcggt caataccagc agcttttgcc
    58081 gcctcgccca cctgtacatg gcaggcttcg ctttcagcgc ccagttccgc catatcgccc
    58141 accaccagca cgcggtagcc cggcatttca gccagtacct gaacagcggc ggtcattgaa
    58201 ccgacattgg cgttgtagga gtcgtcgagc agcaactggt tttctgccag ttggatgggg
    58261 aacagacggc ctggaacagc tttcagattt gccagccccg ctttgatagc atcaagcgtt
    58321 gcgcccacgg acatggagag cgcagcggct gccagcgcat tcgcaatatt gtgacgcccc
    58381 ggcaacggca gcagaacatc gacgctaccg gttggggttt gtagggtaaa ttccgtaccg
    58441 tgcgaggtca catggatatt ggtggcggtg aaatcgctgt tggcggcatt gggtgagaaa
    58501 cgccacactt tgcgtgagcc aattacgctc tgccagttca gccagtcgtt gttgtcggcg
    58561 ttcataatgg cgataccgtt ttccggcagg ccgctaaaga tttcaccttt cgctttcgcg
    58621 acacccgcaa gcgagccaaa accttccaga tgcgccgctg ccaggttgtt gaccagcgca
    58681 gcttccgggc gagtcagact cacagtccag gctatttcgc cctgatggtt cgcgccaagt
    58741 tcaataactg cgtaatcgta ttccggcgtt aagcgcaaca gcgtcatcgg tacaccgatg
    58801 tcgttgttga gattgcctgc cgtataaagc gtgttgccgc actggcttaa aatcgccgcc
    58861 gtcatctctt taacggaggt tttgccggag gaccccgtca gagcaaccac gcgcgccgga
    58921 acttgctggc gaacccatgc agccagttca ccaaacgcca gacgcgtatc cttgacgatt
    58981 aactgcggca ggtcgatgtc cagcggacgg ctaaccagta gtgcgcctgc gccgccagct
    59041 ttcgcctggt cggcaaaatc gtgggcatca aaacgttcgc ctttcagggc aacaaacagg
    59101 cagcccggcg tcagttttcg ggtatcagtg gttacagcat caagggtgat atctgcacct
    59161 tgcagttcac cgttgagaat gtcggtaagt tggctaaggg ttacgctaat catgcaatca
    59221 cccccagcag acgcgccacc gtgacgcgat cggagtagtc cagacgctga ttgccaacaa
    59281 tctggtaatc ttcatggcct ttgcccgcga ccagtaccac atcattctct ttagcctgca
    59341 taacggcgca agtcaccgct tcagcacggc cttccatcac tttggcatgt ccggcatcta
    59401 acattcccgc cagaatatcg ttgatgatgg cacgcggttc ttcggtacgc gggttatcgt
    59461 ccgtcaccac cgccacgtca gcaaactctt cggcaattgc gcccatcagt ggacgcttac
    59521 ctttatcgcg atcgccacca cagccaaaga cgcaccacag cttgcccgca cagtgcaggc
    59581 gcgccgcctg taaggctttt tccagtgcat ccggcgtatg cgcgtaatcc accaccaccg
    59641 tcggtttgcc tggcgcagtg aacacttcca tacgtccgca aaccggttgc agacgcgcgg
    59701 cggttttcag cagatcagcc agtggatagc cgagtgccag cagtgtcgcc agcgcgagca
    59761 gcaggttgct gacgttaaaa gcgcccatca gatggctttc aatttcgcca tcgccccaac
    59821 ttgagctaaa gcgaatcgtc gcaccgctgt cgtgatagtt cacgtcgatc gcttttaacc
    59881 agcgtccgtg acagttcgga ttgatatgat cttccattga taccgcaacc gcgtccggca
    59941 gttttgccag ccagcggcgg cccacttcat cgtcggcgtt aataatcgcc tgaccgcaat
    60001 gatgttcaga ataaagcagc catttcgcag cttcgtagtg ttccatatca ccatgataat
    60061 caaggtgatc gcggcttaag ttggtaaaga ccgacgccgc aaatttcaat gccgccacac
    60121 ggtgctgtac cagcccgtgg gaggaaactt ccattgcgca aaacgtcgcg ccctgctcca
    60181 ccagccccgc cagctcatgc tgaacatcga ctgccgaacc ggtagtattt tctgtcggga
    60241 tcactttccc aagcagaccg ttaccaacgg tgcccattac cgcgctggtt tcgccaagca
    60301 gttggctcca ctgcgccagc agctgggtag tcgtggtttt gccgttggtg cccgttacgc
    60361 ctacgagacg taaattgtca gagggttcat ggtaaaagcg gcccgccagt gcagataaac
    60421 gctcgttgag ctggctgaga tagatgaccg gtacgccgtg catttcacgg atttcaccat
    60481 cggtcgcctc atctttcgcc tctgcaataa tggcagccac accttgcgct atcgcctgcg
    60541 ggatatatcg acgcccgtcc gcctgatgac ctactacagc tacaaagaga tcgcccgccg
    60601 cagccacacg gctgtcgagt gtcatctctc gcagtgctcg cgaaggtgcg tctggcaccc
    60661 acggagcaag aaggtcgcgc aaattacgat ctgccacctg tcccctcgcc ttgattaatc
    60721 acaaattcat ttttatcgcc cgttgtcagc gcatccggct cgatgttcat ggtacgcaat
    60781 acgccgccca tgatggcacc aaagaccggc gcggaaacgg cgccgccgta gtatttaccc
    60841 gcctgcggat cgttgataac aacaaccagc gcgaagcgcg gctgactcgc aggcgcaacg
    60901 cctgcggtat aagcaatata tttattgatg tagcgaccgt ccggcccgac ctttttcgcg
    60961 gtaccggttt taatggcgat acgatagcct ttaatcgccg ctttcacgcc gccgccgcct
    61021 ggtagcgcca cgctttccat catatgcacc acggtgcgaa caatggattc cgggaagaca
    61081 cgttcaccgg gaaccggggg gtcaactttg gtaatcgaca gtgggcgata aatgccgtag
    61141 ctgccgatag ttgcgtagac tcgcgctaac tgtaacggtg ttaccattag cccgtagccg
    61201 aaagagaagg tggccctctc tatgtcagac caccgttgtt tttgaggata taagccactg
    61261 cgttctccga ccaaccccaa attggtcgct tttcccagtc caaaacgtga gtaagtatct
    61321 actaacgctg aggacggcat cgctaacgcc agcttggaaa caccgacgtt actcgacttc
    61381 tgtaataccc cggtcagggt taattcgctg tagcgtgcca cgtctttgat ttcgtggccg
    61441 ttaattcgat aaggaatggt attgagtacc gagttttccc gcaccacgcc acgttgcaac
    61501 gcggtcatta ccaccatcgg tttaaccgtt gagcccggtt caaacacgtc ggtgatggta
    61561 cggttacgca tcgcctcttt cggcgtgccg ctcagattgt tagggttgta tgacgggctg
    61621 ttagccatcg ccagcacttc accggtgttg acatccacca gcacggcgct accggattca
    61681 gccttgttaa aggccaccgc gttgttcagt tcgcgataaa ccagcgcctg caagcgttca
    61741 tcaatactca acgccaggtt gtgcgccgcc tggctgtcag tagaagaaat atcttcaatt
    61801 acgcgaccat agcggtcttt acgcacaatg cgctcacccg gctgcccggt aagccattta
    61861 tcgaaactct tctcaacgcc ctcaatcccc tgactatcga cgttagtaaa gccgatgagg
    61921 tgagcagtca cttcgccgga tggataataa cggcgagatt cttcacgcag atgaatcccc
    61981 ggcagtttca attttttgat gtagtccgcc atgtcagggt ttacctgacg cgccagataa
    62041 ataaagcgcc ctttcgggtt ggcgttaatg cgggcagaaa gctgatccag cggaatattg
    62101 agcgcgttag ccagcgcctt ccagcggtca ccgacgctga taccgccagc gtcatgcact
    62161 tctttcgggt cagcccaaat cgcttttacc ggcacgctca ccgctaacgg gcgaccagaa
    62221 cggtcagtaa tcatgccgcg ggaggtggaa acttgctgaa cgcgaagaga acgcatgtcg
    62281 ccctctttca ccagcatatc cggggagata acttgtaacc acgctacgcg tccgagcaga
    62341 aaagccagcg ccaggagaat acagccgcat aacaacgcaa aacgccaact aataaagttg
    62401 gcatgttctt cctgacgttt tggtttctgc gttttcgccg ctgctttcat gcgtcgcgtt
    62461 tatccttatt tttgcactac gatattttct tgtgacggat caacatgctg catttgcagc
    62521 ttttccgtgg cgatcctttc cacccggcta tggtcgccga gcgcattctc ttcaaggatc
    62581 aggttgcgcc attcaatgtc taaagcatct cgctccagca ccagttgttc gcgctgagcg
    62641 gtcagtaaac gggtatggtg cgccgtggtt accacggtca ccgccgtcaa aataatgcaa
    62701 atgaacaggc agagtggcag cttcccaaat cgcaaaagat cgtcaccgat aacaccaggc
    62761 aatgcatggc gctcgtggct tcccatcgat cctttaactt tgcttagagc ttctgtcact
    62821 ctgctgatca tgcgttcgtc ctctctgcga tacgcagaac tgaactacgg gcacgaggat
    62881 tctcagccac ctcttcttcg cccggcatta acttgcctaa tgctcgcagc tgacggccac
    62941 ccagtttttt gagctgctct tcagtcatcg gtaaccctgc cggaacttgc ggaccgcggc
    63001 tgttttcacg cataaaacgt ttcacaatac ggtcttccag cgagtggaag ctgatgatcg
    63061 aaagccgccc acccggggcc agcacgttga gcgagctttt tagcgcctgc tctatctcct
    63121 ccagttcact gtttacccaa atgcgcaccg cctggaaggt acgagtcgcg ggatgtttaa
    63181 acttatcttt caccggcgtt gcagcagcca cgacttccgc cagttctttg gtgcgggtca
    63241 tcggctgttc gcgattacgc tcgacaatgg cgcgggcaat gcgtttggca aaacgctctt
    63301 caccataggt tttcaatacc caggcgatat cggcttcttc tgcggtttgt agccattcag
    63361 cggctgactg cccacgggtt gggtccatac gcatgtccag cggaccatcg cgcataaagg
    63421 aaaagccgcg ctcagcatca tcaagttgcg gtgaagagac gccaagatcg aggagaatgc
    63481 cgtcgatctt gccgataaga tcgcgctcgg caacgtattc gcccagcgcg gagaaaggtc
    63541 cgtggatgat ggagaagcgc ggatcatcaa tagtcttcgc aacggcgata gcctgcgggt
    63601 cgcgatcgat cgccagcaaa cgcccctctt cgccaagctg cgagaggatc agacgtgagt
    63661 gaccaccgcg accaaaagtc ccatcaatgt agatgccatc aggacggata ttgaggccat
    63721 taacggcttc atccagcagc accgtagtat gtttatagtt ttccatcatt ttatagagac
    63781 aagtcctgca gtcgctccga taagtctccg gtagccaact gctctgcgtc gatatcttcc
    63841 ttgacctgtt gatgccaggt tgtttcatcc cacagctcaa acttgttgaa ctgtccaacc
    63901 agcatcactt cttttgtcag cccggcatgt tgccgcagta ctggcgcgat taacaatcga
    63961 cctgcgccat ccatctgaca ttcgctggca tgacctaaca gtaggcgctg cacacggcgc
    64021 tcaaccgggt tcatgctcga cagacgcgat aatttttgct cgataatttc ccattcaggc
    64081 agggggtaaa gcagcaggca cgggtgatga atgtcaatgg tgcaaaccat ttgaccggca
    64141 gcgttctcaa gcagctgttc ccggtaacgg gtaggcactg ataagcgccc tttgctgtcg
    64201 agattgacta acgttgctcc ccggaacatg ccagcctcac cccttattcc cactttgccc
    64261 cacaatttcc cactgaaagg agtttacgga gcggaggaaa agcttgtcaa gccagtcaca
    64321 aggcataccg aggtaaaaaa cccgatgttt acggctaata tcagcgttgt ttattttacg
    64381 tccaacgtgc gcagcaaatt aacgttatga atagctgtaa gaaaaaatcc ctcatcgtca
    64441 tcaccgtttt aaaatgactc actattttcc acagcaaata taaagtgtca gtttgcgacg
    64501 cgagcggcat tttaggacat atcttccccg gttaacagtc cctgttgcgt ctgtgtggcg
    64561 cttgatgcca gcgggtttgc gcaaaagcaa agaaaagtgc ttgttaaatc ggcaattcat
    64621 ctctgcatgt aacaaaataa tgcaaaaacg cgctgtcgat atcacttaat tattgtgaat
    64681 ttcagctggt gagttaatta acgggcgggt ttaaaagaac ttatcaataa tagtcaggaa
    64741 tagtcttatt tactttaagg atattgatgt ccattcccgt actctacgcg ccagagggaa
    64801 attcacctgg cgcgtatttt tgttcgcggc ttagctacgg ctgagcacac cgcgacgata
    64861 gagattacgt ttaatgcgcg ttaaaccagg ttttggctta cgcggttcgt ccaggcttgc
    64921 caggacaatc tccagcacac gctctgcgac atcgcggtga cgttgagcca ctgccagcac
    64981 cgggcactgt aagaagtcga gcagttcgtt atcgccaaag gtggcaattg ccaggtcaga
    65041 aggcagttta ccatcgcgac gtagcgttac atccatcacc ccttgcaaca acgcaaacga
    65101 cgttgtgaac agcgcctgcg gcatcggatg cgtttccagc catttttcga ataactgggc
    65161 agccgcctcc cgctcatagc tgttggcata caggaaatgc acttcgcgcg gatcatcttt
    65221 ccaggcagta cggaaacctt gttcacgcag gaagctgaca gaaagctccg gcagcgcgcc
    65281 aagataaagc accgtctcgg cgggaaactt acgtaactct tccgccagca tttcggcatc
    65341 atcctgatcg gcaccaacca cgctggtgaa gtgttcacga tcgagggcgc ggtccagcgc
    65401 gacaatcggg aacgggtcgt tagcccagcg ttgataaaaa ggatgctcag gaggcaacga
    65461 cgtcgaaaca ataatggcat cgacctgacg ctgtaaaagg tgctcaatac agcgcatttc
    65521 gttgtctggc tgatcttctg agcaggcaat tagcagttga taaccccgtt gccgcgcctg
    65581 gcgttcaaga tagttagcga tgcgggtata gctggtgttc tccagatcgg ggatcacaag
    65641 accaatagaa cgtgtgcgtc cagcacgaag cccagctgcc acggcgttcg ggtggtaatt
    65701 gtgctcacgc accacagcca tgactttttc aacggttttg tcgctcacac ggtattgctt
    65761 cgctttgccg ttaataacat agcttgcagt ggtccgcgac actcccgcca gccgagcgat
    65821 ttcatccagt ttcacaattg ccccttgcgt aaaatgtaaa aaccatagcc attgtactgg
    65881 tatgggttaa attcattaac atctaagcgc agaaaattaa ctgcggcaac tgcttttatt
    65941 ccaggttccc actgatttcg caaaaaaagc ccaacgtgac atgttgggct gcaaattgcg
    66001 cactgagatc attctcaacg cattatttta tcgccgcgcg aaagtccgac cacaccagag
    66061 cgagcaactt ccacaatttt cgccacatcg cgaatcgatg ctaaaaatgc atcaagctta
    66121 tcgctggtgc ctgctaattg aacggtataa agcgaggggg tgacatcgat aatttgccca
    66181 cggaatattt ccgtattacg tttcacttcg tcacgcccgt aaccgctggc ctgaattttc
    66241 accagcatga tttcccgctc aacatgcgcg ccctgcccca actcactcac gcgcaagaca
    66301 tcgaccagct tgtgtaattg cttttcgatc tgctcaagca ctttttcatc gcccacggtc
    66361 tggatggtca tacgcgataa tgtcggatcg tcggttggcg caacggtcag gctttcaatg
    66421 ttgtagccac gctgggaaaa aaggccaatc acgcgggata acgcgcctga ttcattttcg
    66481 agtaagactg ataatatccg gcgcataatc aggttctctc cgttttgctt aaccacattt
    66541 catccattcc gcccccgcga atctgcatcg ggtagacgtg ctcactgcca tcgacggtaa
    66601 catcaacaaa caccaggcga ttattgcgca cctgttccag cgcctcgcta agtttgcttt
    66661 ccagctcatg cggatgagag atctggatcc cgacgtgacc ataggcttcc gccagacgga
    66721 cgaaatcggg tagtgattgc atataagatt gtgaatgacg gccggaatag atcatgtcct
    66781 gccactgttt caccatcccc agatagcggt tattgagatt caccaccagt accggcaact
    66841 cgtattgcaa cgcggtagac agttcctgga tgttcatctg aatactgccg tcgccagtga
    66901 cgcaaaccac ggtttcttct ggcaacgcca ttttgacgcc cagtgccgca ggtaaaccaa
    66961 aacccatcgt gccgaggcca ccggaattga tccagcgacg cggtttgtcg aatggataat
    67021 aaagtgcagc aaacatctgg tgctgcccga catcggacgt cacgtaagcg tctcccttcg
    67081 tcaaccgcca aagagtctcg atcaccgcct gcggtttaat cttttcactg tgagtgtcat
    67141 atttcaggca ctgacgagcg cgccactgtt caatctgctg ccaccagtcg cggatctcat
    67201 ccagcggttg atgggcggat tcttgcgaca ggagttcaag catttgttcg aggacctggc
    67261 gagcatcccc cacaatcggg atatcagcag tcacggtttt agaaatggaa gtaggatcaa
    67321 tatcgatatg cagaacagtg gcatttgggc agtactttgc cagattattc gtcgttcggt
    67381 catcaaatct taccccgacg gcgaaaatca catccgcgtt atgcatcgtc atattggctt
    67441 cgtaggtgcc gtgcattccc agcatgccca gcgcctgacg atgcgttgcc ggaaatgccc
    67501 ccaggcccat caatgaggaa acaacgggca gattcaacgc ctccaccgtt tctttcaact
    67561 gctgatggca gcccgccgtg attgccccac cgcctacgta gacaaccggt ttttttgccg
    67621 ccaccagcgt ttgcagagca cgcttaattt gccctttatg tccggtagta gtgggattgt
    67681 aagaacgcat actaaccgac tccggccaga catagggtaa tttgttcgcc ggattaagaa
    67741 tatctttcgg taaatcaacg actactggtc caggacgacc acttgccgcc agccagaaag
    67801 cctttttcag cacctgcgga atgtcttccg tttgcttaac cagaaaactg tgtttaacca
    67861 ccggtcgcga aatccccacc atgtcgcact cctgaaaggc atcgtaacct atcaacgagg
    67921 tcgctacctg cccggaaagg acaactaatg gaatggaatc catataagcg gtggcgatgc
    67981 cggtaatcgc attggtcgcc cctggacccg acgttaccag cacgacgccg acttccccgg
    68041 tcgcacgcgc caggccatcg gccatatgca ccgccgcctg ctcatgacga actaatacat
    68101 gatcaatacc acccacggta tgcaatgcat cgtaaatatc aaggactgcg cctccgggat
    68161 aaccgaatac ttgtttaacg ccctgatcga taagcgatcg gacgaccatc tcggctccag
    68221 acaacatctc catggcctgc ctcactgttt gacggaaaaa atgtgtaaaa cacattaacc
    68281 gtccgatctt gcttaagcaa ttggcagcca gagacataaa cgcagggtaa taagaataaa
    68341 caggaggaaa ggtgaaaaga aacgataaaa tcctccatta cgaaggatta aattgacaat
    68401 aaatcggctg aatcccacaa cttacagcaa tttctctatt tttttaatta atcctaaaat
    68461 agagagcaca cacattcaga ataaaattct acattcaata aaaaatgcag aataaaccag
    68521 acattcatgt ctggcctatt ctgcattcag ttagcgtttg caaattgaga ctaattgttc
    68581 ttccatccac tgatggcctt tatcgcgccc ggcagcttca tgccaggaga gataacaggt
    68641 tctgctgttt tgttttaacg gcagcggtaa tacctgtaat tctaaggatt cagcgaactc
    68701 ttcagccagc caacgcggcg caatagcgac caaatgcgtt tgcgacacca cgctaagtac
    68761 gctcatcatt gccatgccct gatacgcgat actggcttgc ttatctaccg tgtcatacca
    68821 aggttgacta aatgacgcga aacgatcgag cgaaaccgcc gcatgttgtt cgttataaac
    68881 atcatgtttc agtaacgggc ccttaattgt tggatgattt ttgctggcta ccagcaccat
    68941 ttcatcttta aataatggca cgctggtaaa ttcaggacga tggaagtctt cataactaat
    69001 cacaaactcc gtttcctgat aacgcagctg atgttcagtg ttctgattta atgaagactt
    69061 gaacataaca tgtatatttg gcgcaatctg ctcaatgtga ttataaatct gcgaggtcag
    69121 aatgctgtct aacgggctgc aaacacaaag atgaaataca cgttcactgc tcgcgggttc
    69181 aaaacctgaa ccaggcaatt cattttgtac tagttgcaat gcctgacgaa ctgagccaaa
    69241 aagttgaaat gcgcgagcag tcggttgaat accacggcca taacgaacaa aaagctcgtc
    69301 attaaacatc accttcaggc gtgcaacagc gttactgacc gcaggttgcg acatacccag
    69361 aacatgagcg gcacgggtaa tgttttgctc ctgcatcacg gcatcgaaaa cggttaataa
    69421 gttgagatcg accatgcgta gctgtggctt gcttaactcc gccgtctctg aatgatctgt
    69481 ttgtacctct ggcatactta actccactgt cacacttaac tccctttccc ttattggaat
    69541 gcagagaaat aatcctgaag aatatgattt atcatgcata taaaataaga
//
LOCUS       DAPHDW010000005        67023 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.005, whole genome shotgun sequence.
ACCESSION   DAPHDW010000005 DAPHDW010000000
VERSION     DAPHDW010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 67023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67023
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.005"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..616
                     /locus_tag="RH057_000288"
     CDS             32..616
                     /locus_tag="RH057_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3126712.1"
                     /translation="MNKVTKTAIVGLLALFAGNAAATDGEIVFDGEILKSACEINDSD
                     KKIEVALGHYNAEQFRSVGDRSPKIPFTIPLVNCPVTGWEHDNGNVEASFRLWLETRD
                     NGTVPNFPNLAKVGSFAGTAATGVGIRIDDAESGNLMPLNAMGNDNTVYQIPADSNGI
                     VNVDLIAYYVSTVEASEITPGEADAVVNVTLDYR"
     gene            696..1391
                     /locus_tag="RH057_000289"
     CDS             696..1391
                     /locus_tag="RH057_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3126713.1"
                     /translation="MSKRTFAVIITLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGTSRAPFIITPPLFRLEAGDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRIKMFYRPAHLKGRVNDAWTSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1421..3937
                     /locus_tag="RH057_000290"
     CDS             1421..3937
                     /locus_tag="RH057_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3126714.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYTFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNRHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWDYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNDNNRNISVGVSGQQWGIGYSLNYQYSRYTDQNNDRALSL
                     NLSIPLERWLPRSRVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDDDNNHN
                     SSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFALIDAN
                     GASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQFVVPNR
                     GAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDETGQQSIVDEGGILYLSGISS
                     KSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            3948..5039
                     /locus_tag="RH057_000291"
     CDS             3948..5039
                     /locus_tag="RH057_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3126715.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACTCSAGKAVKLVYMVSPVLTTTGHQAGYYKLND
                     SLDIKTTLQANDIPGLVTDQTVSVNTRFTQIKSNTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNAPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTIN
                     MRAWPVNLVGGELETGKFQGTATITVIVR"
     gene            complement(5082..5942)
                     /gene="rsmI"
                     /locus_tag="RH057_000292"
     CDS             complement(5082..5942)
                     /gene="rsmI"
                     /locus_tag="RH057_000292"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HDV3126716.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRALEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            6007..8043
                     /gene="lpoA"
                     /locus_tag="RH057_000293"
     CDS             6007..8043
                     /gene="lpoA"
                     /locus_tag="RH057_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HDV3126717.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDAQRREKT
                     LLAVEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTVDGVASPAQASVSDLTGEQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSSLGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            8001..8396
                     /locus_tag="RH057_000294"
     CDS             8001..8396
                     /locus_tag="RH057_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HDV3126718.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEVQARRWLEGKGLRFVAANVN
                     ERGGEIDLIMREGRTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            8416..9006
                     /gene="diaA"
                     /locus_tag="RH057_000295"
     CDS             8416..9006
                     /gene="diaA"
                     /locus_tag="RH057_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HDV3126719.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            9016..9591
                     /gene="yraP"
                     /locus_tag="RH057_000296"
     CDS             9016..9591
                     /gene="yraP"
                     /locus_tag="RH057_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HDV3126720.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(9705..10745)
                     /locus_tag="RH057_000297"
     CDS             complement(9705..10745)
                     /locus_tag="RH057_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV3126721.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWEPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQGGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(10818..11453)
                     /locus_tag="RH057_000298"
     CDS             complement(10818..11453)
                     /locus_tag="RH057_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDV3126722.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTDPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAESMRPEHEGVTILSSSELRKRAE"
     gene            11581..12099
                     /gene="yhbO"
                     /locus_tag="RH057_000299"
     CDS             11581..12099
                     /gene="yhbO"
                     /locus_tag="RH057_000299"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HDV3126723.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGAEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(12079..12522)
                     /locus_tag="RH057_000300"
     CDS             complement(12079..12522)
                     /locus_tag="RH057_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HDV3126724.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            12573..12875
                     /gene="yhbQ"
                     /locus_tag="RH057_000301"
     CDS             12573..12875
                     /gene="yhbQ"
                     /locus_tag="RH057_000301"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HDV3126725.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(12862..13365)
                     /locus_tag="RH057_000302"
     CDS             complement(12862..13365)
                     /locus_tag="RH057_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3126726.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFQVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(13359..13883)
                     /locus_tag="RH057_000303"
     CDS             complement(13359..13883)
                     /locus_tag="RH057_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HDV3126727.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNAQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGLYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            14092..15087
                     /locus_tag="RH057_000304"
     CDS             14092..15087
                     /locus_tag="RH057_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV3126728.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQVAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            15096..15974
                     /locus_tag="RH057_000305"
     CDS             15096..15974
                     /locus_tag="RH057_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HDV3126729.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAATSSADVIYLGEAVCSKRRA
                     TKVGDWLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAAPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            16180..17187
                     /locus_tag="RH057_000306"
     CDS             16180..17187
                     /locus_tag="RH057_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HDV3126730.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSRSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHSPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPNVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYGV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(17305..18549)
                     /gene="mtr"
                     /locus_tag="RH057_000307"
     CDS             complement(17305..18549)
                     /gene="mtr"
                     /locus_tag="RH057_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HDV3126731.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSALGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMITLIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(18703..20592)
                     /gene="deaD"
                     /locus_tag="RH057_000308"
     CDS             complement(18703..20592)
                     /gene="deaD"
                     /locus_tag="RH057_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HDV3126732.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(20585..20665)
                     /gene="yrbN"
                     /locus_tag="RH057_000309"
     CDS             complement(20585..20665)
                     /gene="yrbN"
                     /locus_tag="RH057_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HDV3126733.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(20772..21656)
                     /gene="nlpI"
                     /locus_tag="RH057_000310"
     CDS             complement(20772..21656)
                     /gene="nlpI"
                     /locus_tag="RH057_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HDV3126734.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(21765..23900)
                     /gene="pnp"
                     /locus_tag="RH057_000311"
     CDS             complement(21765..23900)
                     /gene="pnp"
                     /locus_tag="RH057_000311"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HDV3126735.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSRDGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(24147..24416)
                     /gene="rpsO"
                     /locus_tag="RH057_000312"
     CDS             complement(24147..24416)
                     /gene="rpsO"
                     /locus_tag="RH057_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HDV3126736.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(24565..25509)
                     /gene="truB"
                     /locus_tag="RH057_000313"
     CDS             complement(24565..25509)
                     /gene="truB"
                     /locus_tag="RH057_000313"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HDV3126737.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERMVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(25509..25910)
                     /gene="rbfA"
                     /locus_tag="RH057_000314"
     CDS             complement(25509..25910)
                     /gene="rbfA"
                     /locus_tag="RH057_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HDV3126738.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(26074..28746)
                     /gene="infB"
                     /locus_tag="RH057_000315"
     CDS             complement(26074..28746)
                     /gene="infB"
                     /locus_tag="RH057_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV3126739.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQAQREAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSN
                     QQDDMTKNAQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDN
                     AEPTEDSSDYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADRE
                     EARAAVRGGKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIK
                     AMMKLGAMATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAP
                     VVTIMGHVDHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAA
                     FTSMRARGAQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPD
                     RVKNELSQYGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMAS
                     GAVIESFLDKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSI
                     PVEILGLSGVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMT
                     EGEVHEVNIVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASN
                     AILVGFNVRADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLA
                     EVRDVFKSPKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVR
                     NGMECGIGVKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(28771..30258)
                     /gene="nusA"
                     /locus_tag="RH057_000316"
     CDS             complement(28771..30258)
                     /gene="nusA"
                     /locus_tag="RH057_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HDV3126740.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(30286..30738)
                     /gene="rimP"
                     /locus_tag="RH057_000317"
     CDS             complement(30286..30738)
                     /gene="rimP"
                     /locus_tag="RH057_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HDV3126741.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(30945..31021)
                     /locus_tag="RH057_000318"
     tRNA            complement(30945..31021)
                     /locus_tag="RH057_000318"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30985..30987),aa:Met,seq:cat)
     gene            31369..32712
                     /gene="argG"
                     /locus_tag="RH057_000319"
     CDS             31369..32712
                     /gene="argG"
                     /locus_tag="RH057_000319"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HDV3126742.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGVPQVENMENKGQ"
     gene            complement(32720..34345)
                     /locus_tag="RH057_000320"
     CDS             complement(32720..34345)
                     /locus_tag="RH057_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417641.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3126743.1"
                     /translation="MTVFNKFARSFKSHWLLYLCVIVFGITNLVASSGAHMVQRLLFF
                     VLTILVVKRISSLPLRLLVAAPFVLLTAADMSISLYSWCTFGTTFNDGFAISVLQSDP
                     DEVVKMLGMYIPYLCAFAFLSLLFLAVIIKYDVSLPTKKVTGILLLIVISGSLFSACQ
                     FAYKDAKNKKAFSPYILASRFATYTPFFNLNYFALAAKEHQRLLSIANTVPYFQLSVR
                     DTGIDTYVLIVGESVRVDNMSLYGYTRSTTPQVEAQRKQIKLFNQAISGAPYTALSVP
                     LSLTADSVLSHDIHNYPDNIINMANQAGFQTFWLSSQSAFRQNGTAVTSIAMRAMETV
                     YVRGFDELLLPHLSQALQQNTQQKKLIVLHLNGSHEPACSAYPQSSAVFQPQDDQDAC
                     YDNSIHYTDSLLGQVFELLKDRRASVMYFADHGLERDPTKKNVYFHGGREASQQAYHV
                     PMFIWYSPVLGDGVDRTTENNIFSTAYNNYLINAWMGVTKPEQPQTLEEVIAHYKGDS
                     RVVDANHDVFDYVMLRKEFTEDKQGNPTPEGQG"
     gene            complement(34804..34890)
                     /locus_tag="RH057_000321"
     tRNA            complement(34804..34890)
                     /locus_tag="RH057_000321"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34854..34856),aa:Leu,seq:gag)
     gene            complement(34905..35237)
                     /gene="secG"
                     /locus_tag="RH057_000322"
     CDS             complement(34905..35237)
                     /gene="secG"
                     /locus_tag="RH057_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HDV3126744.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNKGSEWENLSAPVKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(35465..36802)
                     /gene="glmM"
                     /locus_tag="RH057_000323"
     CDS             complement(35465..36802)
                     /gene="glmM"
                     /locus_tag="RH057_000323"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HDV3126745.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(36795..37643)
                     /gene="folP"
                     /locus_tag="RH057_000324"
     CDS             complement(36795..37643)
                     /gene="folP"
                     /locus_tag="RH057_000324"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HDV3126746.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(37733..39667)
                     /gene="ftsH"
                     /locus_tag="RH057_000325"
     CDS             complement(37733..39667)
                     /gene="ftsH"
                     /locus_tag="RH057_000325"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HDV3126747.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(39767..40396)
                     /gene="rlmE"
                     /locus_tag="RH057_000326"
     CDS             complement(39767..40396)
                     /gene="rlmE"
                     /locus_tag="RH057_000326"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HDV3126748.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            40522..40815
                     /gene="yhbY"
                     /locus_tag="RH057_000327"
     CDS             40522..40815
                     /gene="yhbY"
                     /locus_tag="RH057_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HDV3126749.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(40971..41447)
                     /gene="greA"
                     /locus_tag="RH057_000328"
     CDS             complement(40971..41447)
                     /gene="greA"
                     /locus_tag="RH057_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HDV3126750.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            41695..43128
                     /gene="dacB"
                     /locus_tag="RH057_000329"
     CDS             41695..43128
                     /gene="dacB"
                     /locus_tag="RH057_000329"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV3126751.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPGDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     EPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(43168..44340)
                     /gene="cgtA"
                     /locus_tag="RH057_000330"
     CDS             complement(43168..44340)
                     /gene="cgtA"
                     /locus_tag="RH057_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HDV3126752.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(44356..45321)
                     /locus_tag="RH057_000331"
     CDS             complement(44356..45321)
                     /locus_tag="RH057_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3126753.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(45448..45705)
                     /gene="rpmA"
                     /locus_tag="RH057_000332"
     CDS             complement(45448..45705)
                     /gene="rpmA"
                     /locus_tag="RH057_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HDV3126754.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(45726..46037)
                     /gene="rplU"
                     /locus_tag="RH057_000333"
     CDS             complement(45726..46037)
                     /gene="rplU"
                     /locus_tag="RH057_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HDV3126755.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            46296..47267
                     /gene="ispB"
                     /locus_tag="RH057_000334"
     CDS             46296..47267
                     /gene="ispB"
                     /locus_tag="RH057_000334"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HDV3126756.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            47495..47773
                     /gene="sfsB"
                     /locus_tag="RH057_000335"
     CDS             47495..47773
                     /gene="sfsB"
                     /locus_tag="RH057_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HDV3126757.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(47821..49080)
                     /gene="murA"
                     /locus_tag="RH057_000336"
     CDS             complement(47821..49080)
                     /gene="murA"
                     /locus_tag="RH057_000336"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HDV3126758.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(49135..49389)
                     /gene="ibaG"
                     /locus_tag="RH057_000337"
     CDS             complement(49135..49389)
                     /gene="ibaG"
                     /locus_tag="RH057_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HDV3126759.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(49549..49842)
                     /gene="mlaB"
                     /locus_tag="RH057_000338"
     CDS             complement(49549..49842)
                     /gene="mlaB"
                     /locus_tag="RH057_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HDV3126760.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(49842..50477)
                     /gene="mlaC"
                     /locus_tag="RH057_000339"
     CDS             complement(49842..50477)
                     /gene="mlaC"
                     /locus_tag="RH057_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HDV3126761.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(50496..51047)
                     /gene="mlaD"
                     /locus_tag="RH057_000340"
     CDS             complement(50496..51047)
                     /gene="mlaD"
                     /locus_tag="RH057_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HDV3126762.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTEPTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(51052..51834)
                     /gene="mlaE"
                     /locus_tag="RH057_000341"
     CDS             complement(51052..51834)
                     /gene="mlaE"
                     /locus_tag="RH057_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HDV3126763.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(51842..52651)
                     /gene="mlaF"
                     /locus_tag="RH057_000342"
     CDS             complement(51842..52651)
                     /gene="mlaF"
                     /locus_tag="RH057_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HDV3126764.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIIAHGSAQALQANPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            52861..53838
                     /locus_tag="RH057_000343"
     CDS             52861..53838
                     /locus_tag="RH057_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HDV3126765.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEVIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQAERQGTDSLTR
                     EQLAELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMGLTAIAI
                     GTSLPELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDY
                     SVMLLVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            53852..54838
                     /gene="kdsD"
                     /locus_tag="RH057_000344"
     CDS             53852..54838
                     /gene="kdsD"
                     /locus_tag="RH057_000344"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HDV3126766.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPGEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            54859..55425
                     /gene="kdsC"
                     /locus_tag="RH057_000345"
     CDS             54859..55425
                     /gene="kdsC"
                     /locus_tag="RH057_000345"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HDV3126767.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            55422..55997
                     /gene="lptC"
                     /locus_tag="RH057_000346"
     CDS             55422..55997
                     /gene="lptC"
                     /locus_tag="RH057_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HDV3126768.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            55966..56523
                     /gene="lptA"
                     /locus_tag="RH057_000347"
     CDS             55966..56523
                     /gene="lptA"
                     /locus_tag="RH057_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HDV3126769.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            56530..57255
                     /gene="lptB"
                     /locus_tag="RH057_000348"
     CDS             56530..57255
                     /gene="lptB"
                     /locus_tag="RH057_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HDV3126770.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            57303..58736
                     /gene="rpoN"
                     /locus_tag="RH057_000349"
     CDS             57303..58736
                     /gene="rpoN"
                     /locus_tag="RH057_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HDV3126771.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            58759..59046
                     /gene="hpf"
                     /locus_tag="RH057_000350"
     CDS             58759..59046
                     /gene="hpf"
                     /locus_tag="RH057_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HDV3126772.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            59164..59655
                     /gene="ptsN"
                     /locus_tag="RH057_000351"
     CDS             59164..59655
                     /gene="ptsN"
                     /locus_tag="RH057_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HDV3126773.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            59701..60555
                     /gene="rapZ"
                     /locus_tag="RH057_000352"
     CDS             59701..60555
                     /gene="rapZ"
                     /locus_tag="RH057_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HDV3126774.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            60552..60824
                     /gene="npr"
                     /locus_tag="RH057_000353"
     CDS             60552..60824
                     /gene="npr"
                     /locus_tag="RH057_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HDV3126775.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            61038..61670
                     /gene="yrbL"
                     /locus_tag="RH057_000354"
     CDS             61038..61670
                     /gene="yrbL"
                     /locus_tag="RH057_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HDV3126776.1"
                     /translation="MIRLSEQNPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(61667..62395)
                     /gene="mtgA"
                     /locus_tag="RH057_000355"
     CDS             complement(61667..62395)
                     /gene="mtgA"
                     /locus_tag="RH057_000355"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
                     /protein_id="HDV3126777.1"
                     /translation="MSKSRLTVFSFVRRFLLRLMVVLAVFWGGGIALFSVAPVPFSAV
                     MVERQVSAWLHGNFRYVAHSDWVSMDQISPWMGLAVIAAEDQKFPEHWGFDVASIEKA
                     LAHNERNENRIRGASTISQQTAKNLFLWDGRSWVRKGLEAGLTLGIETVWSKKRILTV
                     YLNIAEFGDGVFGVEAAAQRYFHKPASKLTRSEAALLAAVLPNPLRFKVSSPSGYVRS
                     RQAWILRQMYQLGGEPFMQQHQLD"
     gene            complement(62392..63045)
                     /gene="elbB"
                     /locus_tag="RH057_000356"
     CDS             complement(62392..63045)
                     /gene="elbB"
                     /locus_tag="RH057_000356"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HDV3126778.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFATLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(63275..65611)
                     /gene="arcB"
                     /locus_tag="RH057_000357"
     CDS             complement(63275..65611)
                     /gene="arcB"
                     /locus_tag="RH057_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HDV3126779.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(65707..66636)
                     /locus_tag="RH057_000358"
     CDS             complement(65707..66636)
                     /locus_tag="RH057_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312117.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HDV3126780.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETGVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALERPWIPPTE"
BASE COUNT        16627 a        18046 c        16354 g        15996 t
ORIGIN      
        1 ttatgtactt gttacaagga taaggttata aatgaataaa gttacaaaaa cagctattgt
       61 gggcttactt gcgcttttcg caggtaatgc cgctgcaacc gatggtgaaa tagtttttga
      121 tggcgagatt ttaaaatctg cttgtgaaat caatgactct gataagaaaa ttgaagttgc
      181 tcttggtcac tataatgccg aacagtttcg ttctgttggc gatcgtagcc caaaaattcc
      241 attcactatt cctttagtta actgcccagt gactgggtgg gagcacgata acggcaacgt
      301 tgaagcgtct ttccgtttat ggctggaaac gcgggataat ggcaccgtcc ctaacttccc
      361 taatctggca aaagtgggct cttttgctgg cacagcggca acaggtgtgg gtatccgtat
      421 tgacgatgcg gaaagcggaa accttatgcc actgaatgct atggggaatg ataacacggt
      481 ttatcagatc ccagcggact ccaatggtat tgtcaatgtt gacctcatcg cttactacgt
      541 atcaaccgtt gaagcatcag aaatcacccc aggggaagca gacgctgtcg ttaacgtaac
      601 gctggattac cgttaatacg ttacggcgtt atctgacctt tcagataacg cccttttcct
      661 tcctctttct cgttgtatca ggttgaaaaa taactatgtc aaaacgaaca ttcgcggtga
      721 taataacctt gttgtgtagc ttctgtatcg gccaggcgct tgcagggggt atcgttttac
      781 agcgaacgcg agtgatctat gatgccagcc gcaaagaggc tgcgttacct gtcgcaaaca
      841 aaggcgcaga aacgccttat ttactgcaat catgggtaga taatatagat ggtacaagcc
      901 gtgccccatt tattataacc ccaccgctat ttcgtcttga agctggcgat gactcatcac
      961 tgcgaattat taaaacagct gataacctgc ctgaaaataa agagtcgctg ttctacatta
     1021 atgttcgtgc cattccagca aagaaaaaat cagatgatgt taatgctaac gagttgacgc
     1081 tggtatttaa aacacggatc aaaatgtttt atcgccccgc acacctgaag ggacgggtaa
     1141 acgatgcgtg gacatcactg gaatttaaac gtagtgacca ttcactcaat atatataacc
     1201 caactgaata ttacgtcgta tttgccggac tggcagtcga taaaaccgat ctcacaagca
     1261 aaattgaata tatcgcgccc ggagaacata aacagttacc acttcctgca tctggcggaa
     1321 agaacgtgaa atgggctgca atcaatgatt atggcggcag ttccgggaca gaaactcgtc
     1381 cactgcaata acaaatataa aaaacacagg tcatcaggga atgccacaac gacaccacca
     1441 gggacataaa cgcacaccga aacagttggc gctcatcatc aaacgctgtt tgccgatggt
     1501 gctcactggc agcggcatgc tttgcactac cgctaacgcc gaagagtatt atttcgaccc
     1561 cattatgctg gaaactacaa aaagtggtat gcaaacaact gatctgtcac gtttttcaaa
     1621 aaaatacgca caactaccag gaacttatca ggttgatatc tggctgaata aaaagaaggt
     1681 ttcacagaaa aaaattacat ttaccgccaa tgcagagcaa cttctgcagc cacagtttac
     1741 ggtagaacaa ctacgtgagc tgggtattaa ggtggatgaa atcccggcgc tggctgaaaa
     1801 agatgacgat agcgtgatca actcgcttga acaaatcatt cccggtacag ctgctgaatt
     1861 tgatttcaat catcagcgac ttaatttgag cattccccaa attgcactgt accgtgatgc
     1921 aagaggttac gtctcccctt ctcgttggga cgatggtata ccaacgctgt ttaccaacta
     1981 ctcgtttaca ggttctgata accgttaccg ccagggcaat cgtagccaac gacagtacct
     2041 gaatatgcaa aatggtgcta attttggccc ctggcgatta cgtaactatt ctacgtggac
     2101 acgcaacgat caggcgtcaa gctggaacac tatcagtagt tatttacaac gtgatatcaa
     2161 ggcgttgaag tctcagttgc ttctgggaga aagcgccacc agcggcagta ttttttccag
     2221 ctacaccttt actggcgtgc aactcgcttc cgacgataat atgttgccaa acagccagcg
     2281 cggatttgcc ccaacggtac gcggtatcgc aaacagtagt gcaatcgtga ctatcaggca
     2341 aaatggttat gtgatctatc aaagcaacgt gccagcgggt gcctttgaaa ttaacgatct
     2401 ctacccctct tccaacagcg gcgatttaga agtcacgatt gaagaaagtg acggtacgca
     2461 acgtcgcttt atccagcctt attcttcatt acccatgatg cagcgacctg ggcatctaaa
     2521 atatagcgcg accgctggac gctatcgcgc tgatgcaaac agtgatagca aggaacccga
     2581 atttgctgaa gccacggcaa tatatggttt gaataatact tttacgctgt atggcggcct
     2641 gctcggttct gaagattatt atgcgctggg gatcggtatc ggcggcacac ttggcgcact
     2701 gggcgcgttg tcgatggata tcaacagagc tgacacccaa ttcgataacc ggcactcttt
     2761 tcatggctat caatggcgta cgcagtacat caaagatatc ccggaaacca acaccaatat
     2821 cgctgttagc tactatcgct ataccaacga tggctatttt agttttgatg aagccaatac
     2881 ccgtaattgg gactataaca gtcgccaaaa aagtgaaatt caattcaaca tcagccagac
     2941 aatatttgat ggggtaagtc tgtatgcctc cggttcgcag caagactatt ggggcaataa
     3001 cgataataac aggaatatct cagttggggt ttccggtcag caatggggaa ttggttacag
     3061 cctgaattat caatacagtc gctacacgga tcaaaataat gaccgcgcac tctctttgaa
     3121 tctcagtatt ccgttagaac gctggttacc gcgtagccgg gtttcctatc agatgaccag
     3181 ccagaaagat cgcccaaccc aacatgaaat gcgtcttgat ggctcactgc tggatgatgg
     3241 tcgcctgagc tatagcctgg aacaaagtct ggatgacgat aacaaccata acagtagcct
     3301 gaacgccagt taccgttcac cttatggcac cttcagtgcc ggatacagct acggtaatga
     3361 cagcagccaa tacaattacg gcgttaccgg cggcgtggtt atccatcctc atggcgtgac
     3421 gctctcgcaa tatctgggca acgcttttgc gcttatcgat gctaatgggg cttctggcgt
     3481 gaggatacaa aactatccgg ggattgctac tgatcccttt ggctatgcag tggttcctta
     3541 tctcacgact taccaggaaa accgtctctc ggtagatact acgcagctgc ccgataacgt
     3601 cgatcttgag caaacaacac agtttgtggt gcccaacaga ggtgcaatgg tagcggcgcg
     3661 tttcaacgcc aatatcggtt atcgcgtact tgttacagtc agcgatcgca acggtaaacc
     3721 gttgcccttt ggcgctcttg ccagcaacga tgagacgggg caacaaagta tcgtcgatga
     3781 gggcggcata ctatatctct ctgggatatc gagtaaatca caaagctgga ctgtacgctg
     3841 gggaaatcag gcagatcaac aatgtcagtt tgcttttagt acaccggatt cagaaccaac
     3901 aacctctgta ttacaaggca cggcgcagtg ccattaagga taaaaaaatg aaaagagcgc
     3961 ctcttataac aggacttttg ttgatatcca catcctgcgc ttatgcctcc tcaggagggt
     4021 gtggagctga cagcactagc ggtgcgacaa attacagcag tgtggttgat gatgttacgg
     4081 tgaaccagac agataacgtg acaggacggg agtttacctc tgccacgcta agtagcacta
     4141 actggcaata cgcctgtacc tgctctgcgg gtaaggcagt taaacttgtc tatatggtca
     4201 gccctgtact taccaccact ggacatcaag caggatatta caaactcaat gatagtctgg
     4261 atattaaaac cacattacag gcgaatgaca ttccaggact cgtgactgac cagaccgttt
     4321 ctgttaacac ccgattcaca cagataaaaa gcaacaccgt atattctgct gcaacccaaa
     4381 cgggtgtttg ccagggtgac acgtctcgtt atggacccgt taatattggt gcaaatacca
     4441 cctttaccct gtatgtcacc aagccatttc tcggctcgat gaccattccg aaaacggata
     4501 ttgccgtcat taaaggcgca tgggtcgatg gaatgggaag cccgtctaca ggtgacttcc
     4561 atgatttagt caagttatcg attcagggaa atctcaccgc cccacagtcg tgcaaaatta
     4621 atcagggcga tgttattaag gttaattttg gattcatcaa tggtcagaag tttaccaccc
     4681 gcaatgccat gccagacggt tttactccag tagactttga tatcacttat gactgtggtg
     4741 atacttcaaa gattaaaaac tcgttgcaaa tgcgcatcga cggtacaact ggggtagtag
     4801 accagtacaa cctggtcgcc agacgaagaa gttcagacaa tgcgcccgat gtcggtattc
     4861 gtattgaaaa tctcggcggc ggagttgcaa atattccttt tcagaacggt atccttcccg
     4921 ttgatccttc cgggcatggc accatcaaca tgcgcgcctg gccagttaat ctggtcggtg
     4981 gtgagctgga aacaggaaaa tttcagggca cagccaccat taccgtcatc gtgcggtaaa
     5041 agaagacgtt gcgttttgat taacgcaacg cttcggcact gttacccctg ctgctccagc
     5101 gcatacttat acagcgcatt tttcttcacg ccgtgaattt ctgcggccag cgccgccgct
     5161 tttttcagcg gcagttctgc ctgtagcagc gccagcgtgc gcagggcatc ggcgggtaag
     5221 tcttcttcct gtgctttatg accttcgaca atcagcacca tttcgccttt gcgacggttt
     5281 tcatcttcct ttacccacgc cagcagctcg ccaacgggcg cgccgtgaat ggtttcccag
     5341 gttttggtca gctcacgcgc cagaaccacg tagcgggatt cgcctaatac cgcaacgata
     5401 tcttccaggc tatctaacag acggtgggta gattcataaa aaatcagcgt gcgcggctcc
     5461 gcttcaatgg cttttagcgc atcacggcgg ccttttgatt tggcaggtaa aaagccttcg
     5521 taacagaaac ggtcagaggg taaacccgct gcgcttaacg cagtgatagc agcacacggc
     5581 ccgggtagcg gcaccacgcg gatccccgct tcacggcagg tacgcaccag atggtagcca
     5641 ggatcgttaa ttagcggcgt tccggcatcg gaaaccagcg caatgttttg cccctcttgc
     5701 agcttcgcca gcagcgtttc agctttttgt tgttcgttat ggtcgtgcag cgcaaacaac
     5761 cgggcattaa tcccaaaatg ttgcagcaat aaaccggtgt gacgagtatc ctcggcggca
     5821 atcagatcaa cggcctgtaa tacctctaac gcacgctggg tgatatccgc cagattgccg
     5881 attggcgtcg gtacaatgta aagctggccc tgagaattat ccgccgattg gtgttgtttc
     5941 attgtgtcgt ccgtattgcc gatttaatat tgagcattgc gtaaaaaaaa tatcactgga
     6001 tacattatgg taccctcaac attttctcgt ttgaaagccg cgcgttgtct gcctgttgtt
     6061 ctggcagccc tgattttcgc cggttgtggc acccatactc ccgatcagtc cactgcttat
     6121 atgcagggta cggcgcaggc tgattctgcc ttttatcttc agcagatgca gcaaagctct
     6181 gatgatacca ggatcaactg gcaattactc gccattcgtg cactggtgaa agaaggtaaa
     6241 accgggcagg cggtggagtt gtttaaccaa ctaccgcaag aactgaacga tgctcagcgc
     6301 cgcgagaaaa cactgctggc ggtagagatt aaactggcgc agaaagattt tgctggcgcg
     6361 caaaacttgc tggcgaaaat cacacctgcc gatttagaac aaaaccagca agcccgttac
     6421 tggcaggcaa aaatcgatgc cagccagggg cgtccttcca ttgatttact gcgcgcgtta
     6481 attgctcagg aaccgctgct cggcgcgaaa gaaaagcagc agaatattga cgccacctgg
     6541 caggcgctct cctccatgac tcaggaacag gcgaatacgc tggtgatcaa cgccgacgaa
     6601 aatattctgc aaggctggct ggatctgcag cgcgtctggt ttgataaccg taacgatccc
     6661 gacatgatga aagccgggat cgccgactgg cagaaacgtt atccgaacaa cccgggcgcg
     6721 aaaatgctgc caacgcagtt ggttaacgta aaagcgttta aaccagcctc gaccaacaaa
     6781 atcgccctgc tgttgccact gaatggccag gcagcggtat ttggtcgcac tattcagcaa
     6841 ggctttgaag cggcgaaaaa tatcggcact cagccagtgg cagctcaggt agctgccgca
     6901 cctgccgcag acgtagctga acaacctcag ccgcaaaccg tggatggcgt tgccagcccg
     6961 gcacaagcct cggttagcga tctgaccggt gaacagcctg cagcccagcc ggtgcctgta
     7021 agcgccccgg cgacaagcac cgcagcggta agcgcacccg caaatccatc cgcagagctg
     7081 aaaatctacg atacctcatc acaaccactt agccagatct taagccaggt tcagcaggat
     7141 ggcgcgagta ttgtggtcgg tccgttgctg aaaaataacg ttgaagagtt gctgaagagc
     7201 aacactccgc tgaacgtact ggcactgaac cagccggaga atatcgaaaa tcgcgtcaat
     7261 atttgttact tcgcgctttc accggaagac gaagcgcgcg atgcagcgcg tcatattcgt
     7321 gaccagggta aacaagcgcc gctggtgctg atcccacgca gttcattggg cgatcgcgta
     7381 gccaatgcgt ttgcgcaaga gtggcagaaa ctaggcggcg gcaccgttct gcaacaaaaa
     7441 tttggttcca ccagcgaatt acgcgcgggt gttaacggcg gttctggtat tgctttaacg
     7501 ggtagcccga ttactcccag agcgacaacc gactccggca tgacgaccaa caatccaacg
     7561 ctgcaaacca cgccaaccga tgaccagttc accaataatg gcggtcgtgt cgatgcggtg
     7621 tacattgtgg caacgccggg tgaaatcgct tttatcaaac cgatgatcgc catgcgtaac
     7681 ggtagccaga gcggtgcaac gctgtacgcc agctcccgca gtgcgcaagg gaccgctggc
     7741 ccggatttcc gactggagat ggaaggcttg cagtacagcg aaatcccgat gctggcaggc
     7801 ggtaatctac cgttaatgca gcaggcactc agcgcggtga ataacgatta ttcactggct
     7861 cgcatgtatg cgatgggcgt cgatgcctgg tcgctggcaa atcatttctc acaaatgcgc
     7921 caggttcagg gttttgaaat caacggtaat accggaagcc tgacggcaaa cccggattgc
     7981 gtgattaaca ggaagttatc atggctacag taccaacaag gtcaggtagt ccccgccagt
     8041 taaccaccaa acagaccggc gatgcgtggg aagtacaagc gcgtcgctgg ctggaaggca
     8101 aaggactgcg atttgtcgcc gctaacgtga acgagcgtgg cggcgagatc gatctgataa
     8161 tgcgtgaagg ccggaccacc gtttttgtcg aggtacgcta tcgccgctct gcgctttatg
     8221 gcggcgcggc agccagtgtg acccgcagca aacaacacaa attattacag actgcccgct
     8281 tgtggctcgc gcgtcataat gggagttttg atactgtgga ttgccggttc gatgtggtag
     8341 ccttcaccgg gaatgaggtt gagtggatta aggatgcctt taatgaccac tcataattaa
     8401 ggtttaagga ttagcgtgca agaaagaatt aaagcttgct tcactgaaag cattcaaact
     8461 caaattgcgg cggcagaggc gcttccggat gccatctccc gtgcagccat gacgctggtt
     8521 cagtctctgc tcaatggcaa caaaatcctc tgttgtggta atggaacttc cgctgccaat
     8581 gcacagcatt ttgctgccag catgatcaac cgtttcgaaa cggagcggcc cagcttacct
     8641 gccattgcac taaatactga taatgttgtc ttaacggcga ttgccaacga tcgcttacat
     8701 gatgaagtgt atgcaaaaca ggtgcgggcg ctgggtcatg cgggagatgt attgttagcc
     8761 atttccaccc gtggcaacag ccgcgatatt gttaaagcag ttgaagccgc cgttacgcgt
     8821 gatatgacca ttgtggcatt gaccggctat gacggcggcg agcttgcagg tttgttaggg
     8881 ccacaggatg tggagatccg cattccttcg catcgtagtg ctcgcattca ggaaatgcat
     8941 atgctgacgg taaattgcct gtgcgatctg atcgataaca cgcttttccc tcaccaggat
     9001 gattaaggag aatacatgaa ggcattatcg ccaatcgcag tccttatttc cgcgctgctg
     9061 ttgcaaggtt gtgttgccgc tgccgtagtg ggtaccgctg ctgtgggtac caaagccgca
     9121 actgacccac gcagtgtcgg cacccaggtg gacgatggta ccctggaagt gcgcgtgaac
     9181 agcgcattgt cgaaagacga acagattaag aaagaaaccc gtattaatgt cacggcgtat
     9241 cagggcaaag tgctgctggt tgggcagtca ccaaacgctg aactttcggc tcgcgccaaa
     9301 cagattgcta tgggcgtaga cggtgccaac gaagtgtata acgagattcg tcagggccag
     9361 ccgattggtc tgggcgaagc atctaacgat acgtggatca ccaccaaagt gcgttcgcag
     9421 ctcttaacca gcgacctggt gaaatcgtcc aacgtgaaag tgaccaccga aaacggtgaa
     9481 gtgttcctga tggggctggt gactgaacgt gaagcgaaag cggcggcaga tattgccagc
     9541 cgggtgagcg gcgtaaagcg ggtaaccaca gcgtttacgt ttattaaata gcagcccctt
     9601 gtaatgcctg atgcgacgct tgccgcgtct tatcaggcat acaacgatgc aaaccgtaga
     9661 tcggataagg cgtttacgcc gcatccgaca tgttttccct caaatcagaa caacagcgcc
     9721 agcccgccga caatcacacc agacactgcc accatcgccc ctgtcagcca taaggctttc
     9781 gccgggaacg ctttgcgcag cataatcagt gacggcaaac tcactgccgg gagcgtcatc
     9841 aacaatgcca gcgccggagc ggttcccata cctgccagca tcatcgtttg tacaatcgga
     9901 atttctgctg ccgtgggaat gacaaacaag catcctgcca ccgccatcgc caccacccac
     9961 atcaggctgt tatcgacagc accatcggca tgggggaata accagacgcg agcggcaccc
    10021 aacaccagta ctgcaaggat gtaaaccggg atcgtactcc agaaaagcgt ccatagcgcc
    10081 ctgccccagc ggctaaaaaa accgccctgt gcttccggta tgtcaatttc gaccggtgcc
    10141 tgcgtttgcg gtgtttcacg cacccatttt tgcaccagcg tcgcaatcag caacaccatc
    10201 accagcccgg ccaccagacg aatcgccgca aaaccccagc cgaggacaaa ccccataaac
    10261 accagcgtcg ccgggtttaa caccggattg cccatccaga atgccagcgc accgcccatc
    10321 gacacctgtt ggcgacgcat tcccgccgcg accggagccg cacagcaggt acacatcatg
    10381 cccggcagcg aaaacagcgt tcccagcagc gtgccgcgaa agcgcgattg cccaagcgta
    10441 cgcaacaacc agtcacgcgg gatcagcacc tgaatcaacg aaccgaggat cacccccagc
    10501 accgccgctt tccataccgc gaggaaatag atcatcgcgt aatccaacgc cgcctgccat
    10561 gggttagcat ccgcctgcgc aaggatagat ttaccgatac tgtgggtttc ggcagcagta
    10621 aacgctttgc cgtagtaagg ttcccatttc acataccaga gaccggcaat gacaacgaga
    10681 aagaaaagcg cgggtttcca ccactgaatg ggcgttgccg cctgagatga agactgacca
    10741 gtcatagcat tccccaggaa taagttatga taattgagcg cgtgaatatt acgctcacta
    10801 tcaattcttg ggaataatta ttcagctctt ttgcgtaatt ctgaagagct taaaatcgtc
    10861 acgccttcat gctcaggccg catcgactct gccagcatga cccgcgccac gtctctcgca
    10921 tcaatggatt tccagttacc tggtaacaaa cggaacagcg gcgcaaaaag cgtttcgttc
    10981 atccgctgtt tgctacgatc gcccagtaac atcgacgggc gagcaatggt caatttcggc
    11041 cagttctggg cgattaatgc ttcttccatc tcccctttga cgcggttata gaaaaacggc
    11101 gagtgggcat tggcacccat cgcactgact accaacatat gctgcgcgcc cagtcgccgc
    11161 ccggttaatg cggtatccac taccagcgtg taatcggcat gaataaacgc ctctttgctc
    11221 cccgcttctc gccgcgtggt gccgagacaa caaaacacaa tgtcgatggg atcggtgacc
    11281 tgcgccaacg cgtcactcag ttgcggatca tggggattaa acaccccggg catatcgccc
    11341 aacggacgtc gcgtcggcgc agcaatggcg ttaactttcg gttcgttaat caacatccgc
    11401 agcaggtgac cgcccaccag ccccgttgcg cctgtaatca gtacctgact catcttcgct
    11461 cctttacaga attgtctgcc ttgcgctcca cggctcatgc accaggctta atagaccatg
    11521 aggtaattat ccccgattgt gggaaattcg cctcatccaa tgcaacaacg cggaggaagc
    11581 atgagtaaga aaattgccgt tttaatcact gatgaatttg aggattcaga atttacttca
    11641 cccgcagacg agttccgtaa agccggacac gaagtgatta ccattgaaaa acaagcgggt
    11701 aaaacggtga aaggcaaaaa aggagaagcc agcgtgacca tcgataaatc catcgatgaa
    11761 gtgacgcctg cggagtttga tgccctgctg ctaccgggcg gccattcacc ggattatctg
    11821 cgtggggaca accgttttgt cacctttacc cgtgattttg tgaatagtgg caaaccggtg
    11881 tttgccatct gtcacggccc gcagttgctg atcagcgccg atgtgattcg ggggcgcaaa
    11941 ctgaccgcag ttaaaccgat cattattgat gttaaaaatg cgggcgcgga attttacgat
    12001 caggaagtcg tggttgataa agatcagctg gttaccagcc ggacaccgga cgatctgcca
    12061 gcgtttaacc gcgaggcgtt acgcctgctc ggtgcctgag tcgcgtagcc aaatcatttt
    12121 tttaccaaag cccagcgtgt tgtcggtgaa tttgatttca tcgagccgga tttcccacac
    12181 cggggccgac agcattctgg caaccggaaa gcgacgattg tacgccttgc gcgcgaggtc
    12241 gctttcctca ccttccagcc tgcggatctc acctttaaac tgcacaccgc gaattaacgc
    12301 taccgttttc ggctgaccgt ttaccgttcc ggcaactgcc gcctgcggcc cgctcatctg
    12361 cgcgtggcgc gttttttctt ccgtcaaaat gtagaaggcg actttctgcg catcaaaaag
    12421 ataaaaggca ttagcgcacc agagttcccc ttcctgctgc acacaccagg tgacaacatg
    12481 ttgttttgcc agccaacggc tgatggcgat gagtgtttcc attgctgttc tcttttatac
    12541 tgtgggcctg accttaacat gcgatgcaga cgatgacacc ctggtttctt tacttgatcc
    12601 gtaccgccga caataagctt tataccggga tcaccacgga tgtcgaacgc cgctatcagc
    12661 agcaccaaag cggcaaaggg gcgaaagcac tgcgcgggaa aggagaactg acgctggcgt
    12721 tttctgctcc agtgggcgat cgttcgctgg cgttacgggc ggaatatcgc gttaagcaac
    12781 tgaccaaacg gcaaaaagag cgtctggtag cggaaggcgc agggtttgca gagctgttaa
    12841 gcagtctgca aaccccggag attaaaagcg attgaaatgc tcgtgatact caaccaggcc
    12901 ggtaacgcca ttcagcgcgt catccgctaa acgatgtacc tggaaggcgc tttcagtgcc
    12961 cggccagcgg caacgcagat cgtgatgcgc cgccagttca aagccgaaac gactgtacag
    13021 cgccggatcg cccagcgtca ccactgcggc atagccgaac tcattaagcg aatcgagtcc
    13081 ttcatagacc agttggcgtg ccagcccctg tccacggtat ttttcatcaa ccgccaacgg
    13141 tgccatgccg acccattgca ggtcttcgcc ctgcacatca accggactaa atgccacata
    13201 gccaatgacc tgaccttcgt catctgtcgc caccagcccc agcgtcagaa agccatcttc
    13261 acgcagatcg tgaaccagct tcgcttccgc atcactttcg aatgagcgac gcagcagggc
    13321 atcaataccc ggcgcatcaa tgggaatttc tactcgaatt agcatggttc acctaccgat
    13381 gtctgtttgg tttcaggcgc ggttttcatt cccgcctcaa caaaatccgc cagttgcagc
    13441 agcatcatgc gtaaggcttt cggcatttgc tccagttcaa tggcgtccat caggtttttc
    13501 acatacagcc ccagctccgt atcgccttca atcaccagcc gacgctggaa gaagagcgta
    13561 tccggatctt gtttacgcgc cgcaatcatc agcagatcgc tggcgtcggc actaaaactc
    13621 acatcagctt gcgcgttctg gctgaccacc agtttgccat tcaccaccga ggtaaaccat
    13681 tgcaggtcaa tatcacgcac atgaatactt aaccagcggc cttcaagaaa ctccagctca
    13741 ccatcatcca gcgcctgacg gaattgccag cttaagacct gctcaagaac ctgccgtttt
    13801 agcgcaaatg gcgtcagttt taccggtaca ctcaacagag atggccccaa atgcacaata
    13861 cgggaacgca gtttatccaa cacgagtttt actccctgtt tcaacaatca tcctattttg
    13921 ccatatcaga aaaataacat agcggtataa atcaacaatt ccatatgaaa ttgctgctac
    13981 caccaataca actttaactg ccttaaatca aaaattgtcg cagcaaggtt aactaaaatc
    14041 ccagttcgtt aacatttttg cgttttgata gcgcaacctt caggaaaaat tatggagctg
    14101 ctctgccctg ccggaaatct cccggcgctt aaggcggcca tcgaaaacgg cgcagatgct
    14161 gtttatatcg ggctaaaaga tgataccaat gcccgtcact tcgccggcct taactttacc
    14221 gagaaaaaat tgcaggaagc ggtgagtttt gtccatcaac atcgccgcaa acttcacatc
    14281 gcgattaaca cttttgcgca tccggacggt tacgcccgtt ggcagcgcgc cgtggatatg
    14341 gcggcgcagc tgggtgccga cgcgctgatc ctcgccgacc tcgccatgct ggaatacgcc
    14401 gccgagcgtt atccgcatat tgagcgccac gtatcggtgc aggcttcggc gaccaatgaa
    14461 gaggcgatta acttttatca tcgccatttt gacgttgccc gcgtggtgct gccgcgcgtg
    14521 ttgtcgattc atcaggtgaa acaactggca cgggtcacac ctgtaccact ggaagtcttt
    14581 gctttcggca gcctgtgcat tatgtcggaa ggtcgttgct atctgtcgtc gtatctgacg
    14641 ggtgagtcgc ccaacaccgt aggcgcgtgt tctccggccc gtttcgtgcg ctggcaacaa
    14701 acgccgcagg ggctggaatc ccgcctgaac gaagtgctga tcgaccgtta tcaggacggc
    14761 gaaaacgcag gttatccgac gctatgtaaa gggcgttatc tggtggacgg cgagcgctat
    14821 cacgcgctgg aagaaccaac cagtctcaat accctggaac tgctgccgga gttaatggcg
    14881 gcgaatattg cttcggtgaa aattgaaggc cgccagcgta gcccggcgta tgtcagccag
    14941 gtggcgaaag tctggcgtca ggctatcgac cgttgtaagg ccgatccgca aaactttgta
    15001 ccgcaaagcg cgtggatgga gacgctcggg tcgatgtccg aaggcacgca aaccactctt
    15061 ggcgcgtatc accgtaaatg gcagtgagaa gagcaatgaa atattcctta gggccagtgc
    15121 tgtggtactg gccaaaagag acgctggaag atttttatca gcaggccgcc accagcagcg
    15181 ccgacgtgat ttatcttggt gaagcggtat gcagcaagcg ccgggcaacc aaagttggcg
    15241 actggctgga gatggcaaaa tcgctcgccg gaagtggcaa gcagattgtg ctctccacgc
    15301 tggcgctggt gcaggcatcc tctgaactgg gcgaactgaa acgctatgtt gagaacggtg
    15361 agtttctgat tgaagccagc gatctcggcg tggtgaatat gtgcgctgaa cgcaaactgc
    15421 cgttcgtcgc cgggcacgcg ctgaactgct acaacgcggt gacgctgaaa atattgctca
    15481 aacagggcat gatgcgctgg tgtatgccgg tggagctttc ccgcgactgg ctggtgaatc
    15541 tgcttaatca gtgcgatgag ctgggcattc gcaaccagtt tgaagtggaa gtcctgagct
    15601 acggtcatct accgctggcc tactccgccc gctgctttac cgcgcgttcg gaagaccgcc
    15661 cgaaagatga gtgtgaaacc tgctgcatta agtatccgaa cgggcgcaac gtgctgtcgc
    15721 aggaaaacca acaagtgttt gtactcaatg gcattcagac catgagcggc tacgtttaca
    15781 acctcggtaa cgagctggca tccatgcagg ggctggttga tgtggtccgc ctgtcaccgc
    15841 agggtactga cactttcgcg atgctcgacg ccttccgcgc taatgaaaat ggcgctgcgc
    15901 cactaccgtt gacggcaaac agcgactgta acggctactg gcggcggctg gcaggactgg
    15961 agctgcaagc ctaagtaaat atgctcgtca tacttcaagt tgcatgtgct gcatctgcat
    16021 tcgttcaccc cagtcactta cttatgtaag ctcctgggga ttcactctct tgtcgccttc
    16081 ctgcaactcg aattatttag agcacagctc actttgttaa caactttaac tactctttaa
    16141 tgcagtatta aagattaatc ggtaacaaag tgagctgtta tgactgataa aaccattgcg
    16201 ttttcgctac tcgatctggc ccccattccc gaaggttctt cagcgcggga agcattctcc
    16261 cgctctctcg acctcgcccg tctggctgaa aagcgcggct atcatcgcta ctggctggcg
    16321 gaacaccaca atatgaccgg cattgccagt gctgcaacat cggtattgat tggctatctg
    16381 gcggcgaata ccaccacgct gcatctgggg tctggcggcg tgatgttgcc taaccactca
    16441 ccgttggtca ttgccgaaca gttcggcacg ctcaatacgc tctatccggg gcgaatcgat
    16501 ttggggctgg gtcgtgctcc gggtagcgat cagcgaacca tgatggcgct acgtcgtcat
    16561 atgagcggcg atattgataa tttcccccgc gatgtcgcgg agctggtgga ctggtttgac
    16621 gcccgcgatc ccaatccgaa tgtgcgcccg gtaccaggct atggcgaaaa aatccccgtg
    16681 tggttgttag gctccagcct ttacagcgcg caactggcgg cgcagcttgg tctgccgttt
    16741 gcgtttgcct cacacttcgc gccggatatg ttgttccagg cgctgcatct ttatcgcagc
    16801 aacttcaaac cgtcggctcg actggaaaaa ccatacgcga tggtgtgcat caatattatc
    16861 gccgccgata gcaaccgcga cgctgagttt ctgtttacct caatgcagca agcctttgtg
    16921 aagttgcgcc gtggcgaaac cgggcaactg ccgccaccga ttcaaaatat ggatcagttc
    16981 tggtcgccgt ccgaacagta tggtgtgcag caggcgctga gtatgtcgtt ggtaggtgat
    17041 aaagcgaaag tgcgtcatgg cttgcagtcg atcctgcgcg aaaccgacgc cgatgagatt
    17101 atggtcaacg ggcagatttt cgaccaccag gcgcggctgc attcgtttga gctggcgatg
    17161 gatgttaagg aagagttgtt gggatagtgt gtcttaacgc gggaagcctt atccgagctg
    17221 gcaacgctgt cctacgtaga cctgataagc gaagcgcatc aggcattgtg taggcagcag
    17281 aaatgtcgga taaggcaccg ctgattactg atacaccggc agtaaattaa agctcgataa
    17341 aatatggacc agtgcgttgc caacgccaaa caccagaatc agcgtaatca tcggcttgcc
    17401 gccccagacg cggaatttcg ggctgccaaa gcgtttacgc gatgcgcggg ctaacagcgc
    17461 cggaacaatt gccgcccaga tggtagctgc taaaccagca taaccaatgg cgtacaggaa
    17521 tccgttcggg aacagtagac cacccacaac aggcggggca aaggtcagca atgccgtttt
    17581 caagcgcccc agggctgagt catcgaaacc aaacagatct gccagatagt caaacaaacc
    17641 cagcgttacg ccaaggaacg aactcgctac cgcaaagttt gagaacacga ccagcagaag
    17701 gtccaggcta cggctgttca gcacgccgct taatgcctgt accagcacat caatattacc
    17761 gcccttctct gcaataccga taaactccgg acgcgggatg ttacccatcg tcgccagcaa
    17821 ccagatggta tacagcgcca gtgccatcag cgtaccgtac accaggcatt tcacgatggt
    17881 tttcggatct ttgccgtaat acttcatcag gcttggcacg ttaccgtgat aaccaaacga
    17941 cgccaggcag aacggcaatg tcatcagcag atacggcgca taagatgcat tgctttctgc
    18001 aacgttgaac aatgtcgcag gctgcacatg ccccaacagg ctgccaaagg tgaggaagaa
    18061 ggtaatgact ttcgccccca gcacaatcgc cgtcatgcgg ctgacggcct tagtactcaa
    18121 ccacaccaca aacgctacca gcagtgcaaa accaaaaccc gccgcccgtg ccgggacgtt
    18181 tagcgacatc tctgcgaagg tgtgatgcag aattgaacca ctggcagaaa tataggcata
    18241 ggtcaggata tagagcacaa aggcaatgga aataccgttg accacgttcc agcctttgcc
    18301 cagcaaatct ttggtgatgg tgtcaaaact cgaaccgatt cggtaattca ggttagcttc
    18361 cagaatcatc aagccggaat gcagcataca gaaccaggta aagatcagcg ccgccattga
    18421 ccagaaaaac cacgccccgg acatgaccac tggcagagaa aacatccctg cgccaataat
    18481 ggtgccgccg ataatcacca cgccgccaag cagcgacggt gacgtttggg tggtggttag
    18541 tgttgccatg agggcttctc tccagtgaaa aatagtgcga ctgcgttgtt atgcattgca
    18601 ctgtaccagt acacgagtac aaaagacaga aaaaaagccc cgatggtaaa aatcggggct
    18661 gtatatatta ttttacagat tgtgttcgct gttcagcgat gattacgcat caccaccgaa
    18721 acgacgacga ccggtagagt catcacgacg cggagcgcgg ccttcacgac gttcgccgct
    18781 aaaacgacga ccatcaccac ggccaccttc acggcgttca ccactgaagt tacgaccgcc
    18841 ttcacgacgt tcgccaccga aaccacgacc accgccacga cgctcaccgc cagtatgcgg
    18901 ctgtgcatcg cccagtaact gcatgttcat cggcttgttg agaatgcgag tacgcgtaaa
    18961 gtgttgcagc acttcacccg gcataccttt cggcagttcg atggtggagt gagaagcaaa
    19021 cagcttgatg ttaccaatgt aacggctgct gatgtcgcct tcgttagcaa tcgcaccaac
    19081 gatatgacga acttcaacac catcatcgcg gcccacttca atgcggtaca gctgcatatc
    19141 gccaacatca cgacgttcac gacgcggacg atcttcacgg tcaccacgcg ggccacggtc
    19201 gttacgatcg cgcggaccac ggtcatcacg gtcacggaat tcacgtttcg gacgcatcgg
    19261 cgcatctggc ggtacgatca gagtacgttc accctgtgcc attttcagca gtgccgcagc
    19321 cagagtttcg agatccagct cttcaccttc tgcagtcggc tgaattttgc tcagcagtgc
    19381 gcggtattga tccagatcgc tgctttccag ctgctgctgt actttagcgg cgaatttttc
    19441 cagacggcgt ttgcccagca gttctgcgtt cggcagttct acttccggaa tagtcagctt
    19501 catagtacgt tcaatgttgc gcagcagacg acgctcgcgg ttctcaacga acagcagcgc
    19561 gcggccagca cgacccgcac gaccggtacg accgatacgg tgaacgtaag actcagaatc
    19621 catcgggata tcgtagttaa ccaccaggct gatacgctca acgtccaggc cacgggctgc
    19681 aacgtcggtc gcaatcagga tgtccagacg accatctttc aggcgttcca gtgtctgctc
    19741 acgcagcgcc tggttcatgt caccgttcag cgcggcactg ttgtagccgt tacgctcaag
    19801 agcttcagcc acttccagag tcgcattttt ggtacgaacg aagataatcg ccgcatcaaa
    19861 atcttccgct tccaggaaac gtaccagtgc ttcgttttta cgcatacccc agacagtcca
    19921 gtagctctgg ctgatgtcag gacgggtagt cacgctggac tgaatgcgca cttcctgcgg
    19981 ctctttcata aagcggcggg taatgcgacg aatcgcttcc ggcatggttg cagagaacag
    20041 agcggtctga tgaccttccg ggatctgcgc cataatggtt tcaacgtctt cgatgaagcc
    20101 catgcgcagc atttcgtcag cttcatccag aaccagaccg ctcagtttag agaggtccag
    20161 agtgccacgt ttcaggtggt ccagcagacg gcccggagta ccgacaacga tctgcggccc
    20221 ctgacgcagg gcgcgtaatt gcacgtcata acgctggccg ccgtacagag caaccacatt
    20281 tacgccgcgc atgtgtttag agaaatccgt cattgcttca gcaacctgta ccgccagttc
    20341 gcgggtcggt gccagcacca gaatctgtgg tgctttcagc tcaggatcaa gattctgcaa
    20401 cagaggtaaa gagaatgctg cagtttttcc gctccccgtc tgggccatac ccagaacgtc
    20461 gcggccattc agcagatgtg gaatacactc tgcctgaatt ggagatggtt tttcgtaacc
    20521 cagatcgtta agggcttcaa ggataggagc cttcaggccc agatctgcaa aagtggtttc
    20581 gaattcagcc atgtagtacg tgtgcctcaa aattaatggc ggccagtcta cataactcat
    20641 catgaaattg atcagcaatt ttcattgaaa agtgtgaacc ggctcaaagt aggtgtatta
    20701 acgaacaaca acgccctcac ccgttaaggt gatggcaatc aaaaaagatt acgggctgat
    20761 gtgtacgtca gctattgctg gtccgattct gccaggtcat cttggtcctg gcccaggagc
    20821 gataattcca acaatgcgta tcggtgctca acaaagttat gaacgttgtt ggcaaccgcc
    20881 agtttgaaca gtgccgtggc gctgtccaaa tcccccagac ttaggtagta cttacctaaa
    20941 tagaagttgg tttcactgag atgctcagcg agcgaggtgt tatccgttgc gtccgccttg
    21001 agcctttcca ttaacgtttg ttcgctaatg ttgcccaggt agaactcgac aatgttccat
    21061 ccccactgtt ccttatccga tttttcgaag tgctgtttca acacttcttt agcctgcttc
    21121 tcatcgagct tctgctcggc gagataaagc cacagactac ggaaaggatc attgggatcg
    21181 tcttgataaa acgccagcag atcatcttgc gctaacttgt cacgaccgcc gtaatataat
    21241 gcgatcccgc gattcaagtg cgcgtagttg taagttggat caagctcaag tacagaatca
    21301 aacgcttcat aggcagcatc aaaattgcct gcctgcgtta aatatatgcc taagtaattg
    21361 aatacttcag gcatatccgg tcggattgcc agcgcttgcg aaaaatcgtt acgcgctaat
    21421 gccctcagac cgagactatc atacaacact ccgcgctcat ataaaagctg tgcgcgttcg
    21481 tcatcggtta aagcccgact ggcaaggatt tgttccatac gtgccagaat cacttcctgc
    21541 tgtaaagtcg gttgcaatgg taccgcgagg acttcacttt tacgccagga agtattactg
    21601 catcctgcaa gcgtaagtgc tgtcgcaacg aaacaccagc gcaaaaaagg cttcatttcc
    21661 cactcccgaa gaccacggtt gaatgaacgt cctgttcccg gttgctaaca aggcgtcctg
    21721 cccggttaaa agccccccgc cgcagcggag ggcaaatggc aaccttactc gccctgttca
    21781 gcagccggag cttccggtgc tgcagcaggt tgagactgct cagtcgcttc tttaatgctc
    21841 agacggatac ggccctggcg atcaacttcc agaactttca ccggtacttc ctgacccatc
    21901 tgcaggtaat cggtcacttt ctcaacgcgt ttgtcagcga tttgagagat gtgtaccaga
    21961 ccttctttac cgccgccgat ggcaacaaat gcgccaaagt caacgatacg ggtcacttta
    22021 ccagtgtaga cgcggcccac ttcgatttct gcagtgatct cttcgatacg acgaatagcg
    22081 tgtttcgctt tctcgccgtc ggtcgctgcg atcttcacag taccgtcatc ttcgatttcg
    22141 atggtggtgc cagtttcttc ggtcagggca cggataacag aaccgccttt accgataacg
    22201 tctttgatct tgtccgggtt gatcttgatg gtatggatac gcggtgcgaa ctcagagata
    22261 tcgccacgcg gcgcgttgat cgcctgttcc attacgccca ggatatgcag acgcgcacct
    22321 ttagcctggt tcagcgcaac ctgcatgatc tctttggtga taccttcaat tttgatatcc
    22381 atctgcaatg cagagatacc gtcgcgggaa cccgcaactt tgaagtccat atcgcccagg
    22441 tgatcttcgt cgcccaaaat gtcagacagt acaacgtagt tgtcgccttc tttcaccaga
    22501 cccattgcga tacccgcaac ggcagctttg atcggcacac ctgcgtccat cagcgccaga
    22561 gacgcgccgc acacggaagc catagaagag gaaccgttgg attcagtgat ttcagacaca
    22621 acacgtacgg tgtacgggaa tttgtccata tccggcatga ctgccagcac gccgcgcttc
    22681 gccagacgac cgtgaccaat ttcacgacgc ttcggagaac cgaccatgcc ggtttcgcct
    22741 acggagtacg gagggaagtt gtagtggaac aggaaggtgt cggtacgttc gcccatcagt
    22801 tcatcaagaa cctgcgcgtc acgagcagta cccagcgttg cggtaaccag cgcctgcgtt
    22861 tcaccacggg tgaacagcgc agaaccgtga gtacgcggca gcacgccagt acgcacatcc
    22921 agaccacgaa tcatatcttt ttcacgaccg tcgatacgcg gttcgcctgc cagtacgcgg
    22981 ctacgaacaa cgtttttctc gatagcgtgc agaatttcac ccagttcgtt ttcgtccagg
    23041 gtttcgtctt cagcaagcag cgtcgcgatg gtttcagatt tgatgacatc aacctgcgca
    23101 taacgctctt gtttgtcggt gatgcggtaa gcatcactca gacgagcttc agccagtgca
    23161 gcaacgcgcg cgttcagcgc ttcgtttacc ggctccggct gccagtccca acgtggttta
    23221 ccggcttctt tcaccagttc attgatgttc tgaataacaa cctgctgttg ttcatgaccg
    23281 aacactactg cgcccagcat ctggtcttcg ctcagcaatt cagcttcaga ttcaaccatc
    23341 agtacagcgg cttcagtacc agcaacaacc agatccagtt tgctctcttt cagctcgtcc
    23401 tgagtcgggt tcagtacgta ctggtcattg atgtaaccta cgcgggcagc accaatcgga
    23461 ccattgaacg gaataccaga cagagacagt gctgcggaag caccaatcat cgcgacgata
    23521 tccggattaa cttgcgggtt aacagaaacc acggtggcga taacctgaac ttcgttgacg
    23581 aagccttccg ggaacagcgg gcgaatcggg cggtcaatca gacgcgcgat cagggtttcg
    23641 ccttcgcttg ggcggccttc acgacggaag aagctacccg ggatacgacc agcagcgtaa
    23701 gtacgctcct gatagttaac ggtcagtggg aagaagtcct gacctggttt ggcttttttc
    23761 tggccaacaa cggtaacgaa taccgcggtg tcatccatgc taaccataac agcggcagta
    23821 gcctgacgag ccatcatgcc ggtttccaga gtcacggtgt gttggccgta ctggaattta
    23881 cgaacgatcg gattaagcaa tgtaatatcc tttctctttc ttagacagta ccttacggca
    23941 ctggtgttaa tacccgatct tctgcgcatc ctcgcgacta atgacaaccc taacccagct
    24001 ctatgtgggt aaagcctctc attagccgcg cgaacctctg caacggaaga tcattcatag
    24061 caacaataca ttagtttcca gtgaattgct gccgtcagct tgaaaaaagg ggccactcag
    24121 gccccctttt ctgaaactcg caagaattag cgacgcagac ccaggcgctc gatgagctgg
    24181 gtgtaacgtg ctacgtcttt acgtttcagg tagtcgagca gtttacgacg ctgagaaacc
    24241 atgcgcagca gaccacgacg gctgtggtga tcttttttgt gctctgcaaa gtggccctgc
    24301 aggtggttga tctgtgcagt cagcagtgct acctgaactt cggtagaacc ggtgtcgttt
    24361 gcgtcacgac caaactcaga aacgatttta gctgttgctt cagtacttag agacatttta
    24421 aaactccaaa gtatatagaa tgaaaggacg ccgatctcta attcagcgat cccagtgtac
    24481 gttacgcaaa gtgttaaaca atttacgcga cgttaagcgg cagtattcta ctcgtagcga
    24541 cctgttatcg caagacggtt aacattacgc cgggtattca accaccaggc gacgaggcgc
    24601 aacgcggcct tcatcgtcaa tttcgcccat accgataaat ttgccgttct caccttctgt
    24661 aacgcgaacc agtccttcca gcggcgcacc ggatgtacga actgggttac catttttgaa
    24721 gtaaacagaa gacgttaacg gaagattcac caccgggtag tccgaagctg gactgtccat
    24781 tggcatcagt aatggatcaa gtaactccgc agccggaata tcctgctgtt cggcttgttc
    24841 gaccagttca cgcagatgtt ccagggtcac catccgttca accggatatt tacttaccgc
    24901 cagacgacgc aggtaaataa catgcgcgcc acaaccgagt ttttcaccca ggtcatcaat
    24961 gatggtgcgg atataagtgc cttttgagca gtgaatttcc agctccagct cattgccttc
    25021 gtggcgaata aacagcaatt cataaacggt aatcgggcgc gcttcacgcg gtacttcaat
    25081 gccctgacgc gcatattcgt acagtttttt accctgatat ttcagtgctg aatacatcga
    25141 agggatctgt tcgatatcgc cacggaaagt atccagcgcc gccgccagtt gctctgcgct
    25201 aaaggttacc ggacgttctt caacgatttg tccgtcggca tcagaagtat cggtacgctg
    25261 tccaagacgc gcaatgaccc gatagcgttt gtcggagtcc agcagatact gggaaaactt
    25321 cgtcgcttcc ccgaggcaaa ttggcaacat gccagttgcc agcgggtcca gcgcaccggt
    25381 atgcccggca cggttggcgt tataaatccg tttcactttt tgcagcgcat cattgctgga
    25441 cataccctgc ggtttatcca gcaacaaaac gccgttaatg tcgcgaccgc gacgacgagg
    25501 acgactcatt agtcctcctt gctgtcgtcc gggttaacac gacgttcttc gtcatgtttg
    25561 accacgctgg tcaccaggtt tgacatgcgc atcccttcaa ccagagagtt gtcgtagaag
    25621 aaggtcagtt ccggcacgat acgcaggcgc atcgctttcc ccagcaggct gcggatgaaa
    25681 ccagaagctt cttgcaacgc tttgatgccc gctttaaccg cgtcttcatc tttgtcgttg
    25741 aggaacgtca catatacttt ggcatacgcc aggtcgcgag acatttcgac accggaaacg
    25801 gtggtcatca tgcccaggcg aggatcttta atttcacgct gcaggatgag agcgatctct
    25861 ttttgcattt cctgcgctac gcgctgcggg cgaccaaatt ctttcgccat aataaattct
    25921 cctgacaaaa aaggggctgt tagccccttt ttaaaattaa tttcaggtgg aagggctgtt
    25981 cacgttggcc tgataagacg cgccagcgtc gcatcaggca gtccatgcag gatgcggcgt
    26041 aaacgcctta tcccgcatgg aaccctaaaa accttaagcg atggtacgtt ggatctcgat
    26101 gatttcgaat acttcgatca catcgccagt gcggacgtcg ttgtagttct taacgccgat
    26161 accacattcc ataccgttac ggacttcgtt aacgtcatct ttgaagcggc gcagggactc
    26221 cagctcgcct tcgtagataa ccacgttgtc acgcagaacg cggatcgggt tgtgacgttt
    26281 aaccacacct tcggtaacca tacagcctgc gatggcacca aatttcggtg atttgaacac
    26341 gtcacgaact tccgccagac cgataatctg ctgtttcagt tccggagaca gcataccgct
    26401 catcgccgct ttcacttcgt caatcaggtt atagatgacg gagtagtaac gcagatccag
    26461 gctttccgct tcaatcactt tacgtgcaga ggcatcagca cgtacgttga agccaaccag
    26521 gatggcgttg gacgccgcag ccagggtggc gtcggtttcg gtgatacccc ctacgccaga
    26581 accgatgatc ttcactttaa cttcgtcagt agacagtttc agcaaggagt cggagatcgc
    26641 ttccacagaa ccctgtacgt ctgccttcag gacgatattc acttcgtgaa cttcgccttc
    26701 ggtcatgttg gcaaacatgt tctcgagttt agatttttgc tgacgcgcca gtttaacttc
    26761 gcggaattta ccctgacgat agagtgcaac ttcacgcgct ttcttctcgt cacgtacaac
    26821 ggtaacttca tcacccgcag ccggaacgcc ggacaggccg aggatttcca ccggaatgga
    26881 cggacccgct tccagcactt cctgacccag ttcgttacgc atcgcacgaa cacgaccgta
    26941 ttcgaagcca cacagaacga tatcgccctt gtgcagagta ccttcacgta ccagaacggt
    27001 agcaaccgga ccacgacctt tatcgaggaa ggattcgata accgcaccgc tcgccatacc
    27061 tttacgtacc gcttttagct ccagaacttc cgcctgcagc aggatagcgt ccagcagttc
    27121 atcgataccg gtacccgctt tcgcagatac gtgtacgaac tggctttcac cgccccactc
    27181 ttccggcagg atgccatact gggagagttc gttcttaaca cgatccggat cggcttctgg
    27241 tttatcgatc ttgttcactg caaccaccac cggtacctgc gccgctttcg cgtgctggat
    27301 tgcttcgatg gtctgcggca tcacgccgtc gtcggcagca acaaccagga ctacgatgtc
    27361 cgttgcctgc gcaccacgtg cacgcattga agtaaacgcg gcgtgccccg gggtgtccag
    27421 gaaggtgatc atgccgtttt cagtttcaac gtggtatgca ccaatgtgct gggtaatgcc
    27481 gcccgcttcg ccagaggcca ctttcgttga acgaatgtag tccagcagag aggttttacc
    27541 gtggtcaacg tgacccatga tggtcacaac cggcgcgcgc ggttcagccg cagcccccgt
    27601 gtcacggtcg ctcattaccg cctcttccag ctcgttttca cgacgcagga taactttgtg
    27661 gcccatctct tcagcaacca gctgtgcggt ttcctgatcg ataacctggt tgatggttgc
    27721 cattgcgccc agtttcatca tcgctttgat gacctgagag cctttaaccg ccatcttgtt
    27781 cgccagttcg ccaacggtga tggtttcgcc gatcacaacg tcacggttaa cggcctgagc
    27841 tggcttctgg aagccttgct gcagggaaga acctttacgt tttccgcctt taccgccacg
    27901 tactgctgcg cgtgcttctt cacgatcagc ttttgattca gcgtgtttgt tgcctttctt
    27961 cggacgcgct gctttcgcgt tacgaccacg gccacggccg ccttcgactt cacgatcgct
    28021 ttcgtcttct gcctggcgag catgttgaga agttgtgaca tgataatcgc tggaatcttc
    28081 agtcggttcc gcgttatcag tccatttgtt ttcttccgcc atacgacgtg cttcttcagc
    28141 aacgcgacgt gcttcttctt cgagtttacg acgcgcttct tcttcagctt tacgcttgag
    28201 ctctgcagct tcctgctcac ggcgggcttt ttcagcctgg gcgtttttag tcatatcgtc
    28261 ttgttgattg ctcactttgt ctttttccgc agcttcacgt ttcgcttgtt cagcagcttc
    28321 acgcttagct tgttctgcgg cctcacgttc agctttttgt tgcgcctcgc gtttagccga
    28381 ttcttctgcc tcacgacggg cttgctcttc cgcttcacgc tgcgcttgct cttccgctgc
    28441 aaggcgttca gcctcttgcg gatcgcgttt cacaaaggtg cgtttcttgc ggacttcgat
    28501 ttgtaccgat ttgctttttc caccggtacc aggaatgtta agggtgctgc gtgttttacg
    28561 ttgcagcgtc aatttgtccg ggcctgaatt tttctgattc aggtggtcaa tcaaagtctg
    28621 tttctcttgt gcagacacag agtcgtcagc agacttccgg atacctgcat cagcaaattg
    28681 ctgtaccagg cgttccacgg aggtctgtcg ctctgcggcc agcgttttaa tcgttacatc
    28741 tgtcatgctg ttccttcctg ctacagttta ttacgcttcg tcaccgaacc agcaaatatt
    28801 acgggcagcc ataatcagtg ctccggcttt ttcgtcggtc aacccttcga tatcagccag
    28861 atcatcaatg ccctgttcgg cgagatcttc cagcgtacaa acgccacggg cggccagttt
    28921 gaatgccaaa tcacgatcta ccccttcaag gttcagcaga tcgtcagccg gtttgttatc
    28981 accgaggctt tcttcctggg cctgtgcaat ggtggccagt gcatttttag cacgctcgcg
    29041 cagtgcttca acggtcggct catcaaggcc ttcgatttcc aacagctctt tcatcggcac
    29101 ataggccagt tcttccagcg tcgagaagcc ttcttctacc agaacagtcg cgaagtcttc
    29161 gtcgatgtcg agatatttgg tgaaggtgtc gatcgctgcg tgcgcttccg cctgatgctt
    29221 agcctgcagg tcgtcaacgg tcatcacgtt gagttcccaa ccgctcagct gcgaagccag
    29281 acgcacgttc tgaccgttac ggccaatcgc ctgcgccagg ttaccggctt caacggcgat
    29341 atccatggtg tgtttatctt catccaccac gatagaagca acgtctgccg gtgccattgc
    29401 gttaatcaca aactgcgccg ggttatcatc ccacaggacg atatcgatac gctcgccgcc
    29461 cagttcagta gaaaccgcct gaacacgcgc gccacgcata cctacacagg cacctaccgg
    29521 atcgatacgt ttatcgttgg ttttcaccgc gattttcgca cgagaacccg gatcgcgagc
    29581 cgctgcttta atttcaatca cttcttcgcc gatttctggc acttcaatac ggaacagttc
    29641 gatcagcatt tccggcttgg aacgagtgac gaacagttgc gcgccacgcg cttccgggcg
    29701 aacggaatag agcacgccac gaacgcggtc gccagggcgg aagttttcac gcggcagcat
    29761 atcttcgcgc aggatcacgg cttcagcatt gttgcccaga tccagagaga tgttgtcgcg
    29821 gtttactttt ttcaccacgc cggtgatgat ttcaccttcg tgttcacgga actgatcaac
    29881 caccatcgca cgttcggctt cacgcacttt ctgcacgata acctgttttg ccgtctgggt
    29941 agtgatacgg tcaaaggtaa cagactcaat ctgatcttca acgtaatcgc ccaggttcag
    30001 gctttcatct tcataacgtg cggcttcaag ggtgatttcc ttggtcggct gggtgacttc
    30061 atcaacaact aaccagcgac ggaaggtgtc aaaatcaccg cttttgcgat cgatctgtac
    30121 gcggacgtcg atctcttgtt catatttttt ctttgttgct gtcgccagcg cgctttccaa
    30181 tgcttcgaaa atcttctcgc gaggtagcgc cttttcattg gatacggctt caactacagc
    30241 caaaatttct ttgttcatcg cgggcttttc acctcatcca gactattaaa agtggggaac
    30301 caggttcgcc ttctggatat tactcagcgc gaacacttca tctttacctt cgacggtaac
    30361 tgtgatcatt tcaccgtcta ccgctttgat aacgccctgc catttacgac ggttttgtac
    30421 cgccatacgg agaaccagag tcacctcttc tccgacaaaa cgggcgtagt gttcagccgt
    30481 gaacagtggg cgatcgagac ccggtgagga gacttccagg ttataagcaa cggtgatggg
    30541 atcttcaaca tccagcacag cacttacctg gtggctcaca tcagcacaat catcaacatt
    30601 gatgccatct tcactatcaa tatagatgcg cagtgtggat gtgcgaccgc gaataaattc
    30661 gatgccaacc agttcaaaac ccagggcctc aactggcgca gtaatcatct ctgttaattt
    30721 ttgctctaat gtggacaagc ccacccccaa gacataaaaa aagggcctaa agcccagtta
    30781 ttctgtagtc agataacaaa aaaccccgat aaatcggggc tttatataac tgaaccctat
    30841 aaccgcaact gcggtctgga gcactttcca gaaggatttt ttcaaatccc actacgaagg
    30901 ccgaagtctt cacagtatat ttgaaaaagg actctaaggg aaagtggttg cgggggccgg
    30961 atttgaaccg acgaccttcg ggttatgagc ccgacgagct accaggctgc tccaccccgc
    31021 gcctgaaacg tggcaaattc tactcgtttt gggtaaaaaa tgcaaatact gctgggattt
    31081 ggtgtaccga gacgggacgt aaaatctgca ggcattatag tgatccacgc cacattttgt
    31141 caacgtttat tgctaatcat gtgaatgaat atccagttca ctttcatttg ttgaatactt
    31201 ttgccttctc ctgctccccc ttaagcgcat tattttacaa aaaacacact aaactcttcc
    31261 tgtctccgat aaaagatgat taaatgaaaa ctcatttatt ttgcataaaa attcagtgag
    31321 agcggaaatc caggctcatc atcagttaat taagcagggt gttattttat gacgacgatt
    31381 ctcaagcatc tcccggtagg tcaacgtatt ggtatcgctt tttccggcgg tctggacacc
    31441 agtgccgcac tgctgtggat gcgacaaaag ggagcggttc cttatgcata tactgcaaac
    31501 ctgggccagc cagacgaaga ggattatgat gcgatccctc gtcgtgccat ggaatacggc
    31561 gcggagaacg cacgtctgat cgactgccgc aaacaactgg tggccgaagg tattgccgct
    31621 attcagtgtg gcgcatttca taacaccacc ggcggcctga cctatttcaa cacgacgccg
    31681 ctgggccgcg ccgtgactgg taccatgctg gttgctgcga tgaaagaaga tggcgtgaat
    31741 atctggggtg acggtagcac ctataaagga aacgatatcg aacgtttcta tcgttatggt
    31801 ctgctgacca atgctgaact gcagatttac aaaccgtggc ttgatactga ctttattgat
    31861 gaactgggcg gccgtcatga gatgtctgaa tttatgattg cctgcggttt cgactacaaa
    31921 atgtctgtcg aaaaagccta ctccacagac tccaacatgc ttggtgcaac gcacgaagcg
    31981 aaggatctgg aatacctcaa ctccagcgtc aaaatcgtca acccaattat gggcgtgaaa
    32041 ttctgggatg agagcgtgaa gatcccggca gaagaagtca cagtacgctt tgagcaaggt
    32101 catccggtag cgctgaacgg taaaaccttt agcgacgacg tagaaatgat gctggaagct
    32161 aaccgcatcg gcggtcgtca cggcctgggc atgagcgacc agattgaaaa ccgtatcatc
    32221 gaagcgaaaa gccgtggtat ttacgaggct ccggggatgg cactgctaca tatcgcttat
    32281 gaacgtctgt tgaccggtat tcacaacgaa gacaccattg agcagtatca tgcacatggt
    32341 cgtcagttgg gccgtctgct gtaccagggg cgttggtttg actcccaggc gctgatgctg
    32401 cgtgactctc tgcaacgctg ggttgccagc cagatcactg gtgaagttac cctggagctg
    32461 cgccgtggga acgattattc aatcctgaat accgtctcag agaacctgac ctacaagcca
    32521 gagcgtctga cgatggaaaa aggcgactcg gtgttctcgc cagatgatcg tattggtcaa
    32581 ttgaccatgc gtaatctgga tatcactgat acccgagaga aactttttgg ttatgccaaa
    32641 actggcctgc tctcctcctc tgccacttca ggcgtgccgc aggtggagaa tatggaaaac
    32701 aaaggccagt aattcgacat caaccctgcc cttcaggggt ggggttacct tgcttatcct
    32761 ctgtaaactc ctttctgagc atcacataat cgaaaacatc atggtttgca tccacaaccc
    32821 gtgagtctcc tttatagtgt gcaatcactt cctcaagcgt ttgcggctgt tccggctttg
    32881 ttacccccat ccacgcatta ataaggtaat tattgtaagc tgtcgaaaag atgttgtttt
    32941 ccgttgtgcg atccacgcca tccccaagaa cggggctata ccagataaac atcgggacat
    33001 gatatgcctg ctggctagcc tccctgcctc catgaaaata gacgttcttc ttcgtagggt
    33061 cacgttccag gccgtggtcg gcaaaataca tgaccgaggc gcggcgatct tttaataatt
    33121 caaaaacctg acctagcaaa ctatctgtgt aatgaatgga gttgtcatag caggcatcct
    33181 gatcgtcctg cggttgaaac acggcgctgg attgcggata ggcgctacaa gccggttcat
    33241 ggcttccatt taaatgaaga acaatcagtt tcttttgctg cgtattttgc tgtaatgctt
    33301 gcgataaatg cggcaacaac aattcatcaa atcctctgac ataaactgtt tccatggcgc
    33361 gcatggcgat actggtaact gctgtaccat tctgccgaaa agcggattgc gagcttagcc
    33421 agaaagtctg aaatcctgct tgattagcca tattaataat gttgtcgggg taattatgaa
    33481 tgtcatgact caaaacagaa tcagcagtta aagaaagggg aaccgacagc gcagtgtaag
    33541 gtgcgccgct tattgcttga ttaaacagtt tgatctgttt tctttgtgct tcaacttgcg
    33601 gtgtcgtaga gcgtgtatat ccatacaaag acatattgtc gacacgtaca gactccccca
    33661 caatcaacac gtaggtatca atacctgtat ccctgactga taattgaaaa tacggcaccg
    33721 tgtttgcaat tgagagtaat ctttgatgct ctttcgctgc taaagcaaaa tagttgagat
    33781 tgaaaaacgg cgtataggta gcaaatcgcg acgctagtat atatggactg aacgcttttt
    33841 tattttttgc atctttataa gcaaattgac aagcggaaaa taaactgccc gaaatgacaa
    33901 tcagcaataa tattcctgtc actttttttg tcggcaagga aacatcatat tttattatta
    33961 ctgccaaaaa aagaagggat aaaaaagcaa aggcacatag ataagggata tacatcccca
    34021 gcattttgac aacttcatcc ggatcactct ggagcacact aatcgcaaat ccatcattga
    34081 aagttgtacc aaaggtacac catgaataga ggctaatact catgtctgcc gcagtcagta
    34141 acacaaatgg tgcggcaaca agcaggcgaa gcggaaggga tgatatacgt tttacaacca
    34201 ggatggtcag gacgaagaac agcaatcgct gaaccatatg cgcgccggaa gaggcgacta
    34261 agttcgtaat accaaaaaca ataacacaaa gatacaacaa ccaatgagat ttaaaacttc
    34321 tagcaaattt gttgaatact gtcatccaga aatccaagtt cacaaccaat ataaacaatt
    34381 atcaacaatg ttcaatttta tatcagacat gtttttcaac tctgatccat atcagaccga
    34441 aacactgtta gttttttctt tttaaatgat gcggccattg cgttctcagt aattaaataa
    34501 cattatcaat gcgtctctaa acattacgca gaacgtagcc atcagtagtt atactgacag
    34561 gagcaggatg gtttatcgag aagtaaacat atccgtattc gtaccggaac tggagttgac
    34621 acaataaagt gccaattatg tcagcagaag ggaaaagttt ataactaagg cgtaccggca
    34681 ccatcgtttc aaggtaccag ctacgagtaa agcaactgga cgagatacag atacctgaca
    34741 accattcctc agaccaggac caaaacgaaa aaagacgctt ttcagcgtct cttttctgga
    34801 atttggtacc gaggacggga cttgaacccg taagccctat tgggcactac cacctcaagg
    34861 tagcgtgtct accaattcca ccacctcggc acggatacta ctttttagtt cgggatatcg
    34921 ctggtcggct tagccggagc agctggttga gtttgttcgg ttttcaccgg tgcactcaga
    34981 ttttcccatt cgctaccttt attggttttg ttgctattga tgttacccag caccagactg
    35041 atgatgaaga ataacgttgc cagcagcgcc gtcatgcggg tcatgaagtt accagaacca
    35101 cttgaaccaa acagcgtagc ggaagcgcct gctccgaagg aggctcccat atcagcgcct
    35161 ttaccttgct gcagcatgat cagaccaaca aggccaattg ccacaataag gaaaactact
    35221 aaaagagctt cgtacataat caacctgttc cttgcggagt tgccgcgtac caatgcttca
    35281 accaataaag cgggagtttt taatctttcc cactgaagcg ggtgtgaata ctaaccaaag
    35341 cgaatgacct tcgcaagggc aattttatcg cattgtatca actgcggaaa aaaacagcaa
    35401 aaccgatgtg ttcgctgaga aaaaaggcga caatcgccgc ctttttagcc agttatctaa
    35461 cgctttaaac ggcttttact gcatcggcga tgcggtgtgc aaattcagtc acctgcgctt
    35521 cgtcttcgcc ttccaccatc acgcgaatta acggttcggt gccggattta cgcagcaaca
    35581 cgcgtccacg gttgcccagc gcagcttcaa cctctgcggt cacggcttta actgactcat
    35641 gctcaagtgg atcgccgcta cctgcggtgt aacgtacgtt aaccagaatc tgcgggaaca
    35701 ttttcatgcc gctgcaaagg tcgtgcaggc tcatatggtt acgtgccatc gccgccagca
    35761 cctgcaagcc agcaacgatg ccgtcaccgg tagtagtttt atccagtagg atcacatgac
    35821 cggaattctc tgcaccgata cgccagcctt tctcctgcat tttttccagt acgtagcggt
    35881 cacccacttt cgcgcgcgca aatggaattc ctaactgttt cagcgccagt tcaagcccca
    35941 tgttgctcat caatgtaccc acagcgccac cacgcagctg gccctgacga agaccttcac
    36001 gcgcgatgat atacatgatc tgatcgccat cgactttatt gccttcatgg tcgaccataa
    36061 tcacgcgatc gccatcgccg tcgaaggcaa taccgagatc cgctttttca gccagcacac
    36121 gagcctggag cgcgcgaacg tcggtagccc ccacttcggc attgatgttt acaccgtttg
    36181 gctcacaacc gatagcgata acgttcgccc ccagttcgcg cagcacgttc ggcgcgatgt
    36241 gataagtcgc accgtttgca caatccacca caatcttcag ttcactgagg ctaagttcgt
    36301 tcgggaacgt ggctttgcaa aactcgatat agcgacccgc ggcatcaacg atacggctgg
    36361 ctttacccag ttctgccgaa tcaacgcagc tgatctcctt ttccatttcc gcttcgatgg
    36421 cctcttctac cgcatccggc agtttggtgc cgtcgataga gaagaattta atcccattat
    36481 cgtagaacgg gttatgcgat gcagatatca caattccggc ctctgcgcgg aaggtacgcg
    36541 tcagataagc cacggccggt gttggcatcg ggccagtgaa gagtgcggaa aggcccgctg
    36601 ccgccagacc cgcttccagt gctgactcca gcatatagcc agaaatacgc gtgtctttac
    36661 caataataat cttacgggag ccgtggcgcg ccagcacttt acccgcggcc caacccagct
    36721 taagcacaaa atcaggtgtg atcggcgcat cccctacacg accacgaatc ccatcggtac
    36781 cgaaatattt acgattactc atagcgtttg ttttcctttg cagacagagt ggcttccacc
    36841 acccgcatcg cttctacggt ttctttgacg tcatgaacac gaatgatgtg cgcgccttgc
    36901 attgcggcaa tgaccgcaca ggccagacta ccgctcaggc gctcggacgg ccccacgttc
    36961 agcagctgcc caatcatcga ttttcgtgac atacccacca acagcggcag gttgaaatgg
    37021 tgaaattcag ccaggcgcgc cagtaatgaa tagttatggg agagattttt accgaaaccg
    37081 aatccggggt cgagcaacaa tttctctttt gcgatacccg cctgctcgca acgtgctatt
    37141 tgctcaataa agtagcgatt cacttctgca aagacatcgt catacttcgg agcttcctgc
    37201 atggtttttg gatttccctg catatgcatc agacaaaccg gtaaaccggt ttctgcagcc
    37261 gcctccagag cgccaggttc ggaaagggag cggatatcat taataatgtg agcgccaact
    37321 ttcgctgact cacggatgac ttctggtttg gatgtatcga ccgagatcca gacttcgaag
    37381 cgttgagcaa ttgcctcaac cacaggaata acacgttgca actcttcttc aacgctaact
    37441 tccgccgccc ctgggcgcgt ggactcgcca ccaacgtcaa tgatcgtcgc gccagcattg
    37501 atcatcagat tcgcatgttt caccgcatct atcagcgagt tatgcgtgcc accatccgaa
    37561 aaggaatcag gcgtgacgtt gaggatcccc attacgtgag gatggctaag gtccagtgaa
    37621 gtaccctggg caaagagttt catgatgtta tccctggtat gaattgataa gaaaaaaccc
    37681 cggagcacgc cccggggttt tcggtacaaa tacagtcatc tgatgcggga acttacttgt
    37741 cgcctaactg ctctgacatg gtgttacccg ggttcggcgt acgcggttca tcaaccggac
    37801 gaggagcctt tggactacca ttgtcgccag aattgttaga agcgcctggt tcttcccagc
    37861 ccgctggcgg acgtacatcg cgacgtgcca tcaggtcatc aatctgcggt gcgtcgatag
    37921 tctcatattt catgagagca tctttcatcg catgcagaat atccatattg tcggtcagaa
    37981 gctgacgcgc acgattatag ttacgctcaa tcagtgcttt cacttcctgg tcgatgatac
    38041 gtgcagtttc atcggacata tgtttcgctt tcgctacgct acggccgagg aatacttcac
    38101 cttcttcttc cgcgtacagc agtggaccca atttctcaga gaagccccac tgggtcacca
    38161 tgttacgtgc cagattggtc gcaactttaa tatcgttgga cgcaccggta gaaacatgtt
    38221 ccggcccata gatgatctct tctgccagac gaccaccgta cagcgtagaa atctggcttt
    38281 ccagtttctg acggctggcg ctgattgcgt cgccctcagg caagaagaaa gtcacaccca
    38341 gcgcacgacc gcgtgggata atcgtcactt tgtgcaccgg atcgtgttcc ggcaccaggc
    38401 gaccgataat cgcatgaccc gcttcgtggt aagccgttga ttctttctgc gcttccgtca
    38461 tcaccatgga gcgacgttcc gcacccatca tgattttgtc tttcgctttc tcgaactcaa
    38521 ccatcgacac aacgcgtttg ttgccacgag cagcgaacag tgccgcttcg ttcaccaggt
    38581 tcgccaggtc agcaccggag aaaccaggag taccacgggc aatgattgcc gcgtcgatat
    38641 cgggggccaa tggtacgcga cgcatgtgaa ctttcaggat ctgctcacga ccgcgaacat
    38701 ctggcaagcc gaccacaacc tgacggtcga aacggccagg acgcagcagg gccgggtcga
    38761 gaacgtccgg acggttagtc gcggcgataa cgatgatacc ttcgttacct tcgaagccat
    38821 ccatctcaac cagcatctgg ttcagagtct gttcacgttc atcgtgacca ccgcccagac
    38881 cagcgccacg ctggcggcct acggcgtcga tttcatcgat aaagatgatg cacggtgccg
    38941 ctttcttcgc ctgttcgaac atgtcacgaa cacgggatgc acccacaccg acgaacattt
    39001 ctacgaagtc agaaccggag atagtaaaga acggaacttt cgcttcgcct gcaatcgctt
    39061 tcgccagcaa cgttttaccg gtacccggag gaccgaccat caggacgcct ttcgggatct
    39121 taccgccgag tttctggaag cggctcggct cgcgcagata ctctaccagt tcagcaactt
    39181 cttcttttgc ttcgtcgcag cccgcaacgt cagcaaaggt cgttttgatc tgatcttccg
    39241 tcagcatgcg cgctttgctc ttaccaaacg acatggcacc tttgccaccg ccgccctgca
    39301 tttgacgcat gaagaagatc cagacaccaa ttagcaacag catcgggaac caggagatga
    39361 agatagaagc cagcaggctt ggttcttcag gcggttcacc gacaaccttg acgttcttgg
    39421 tcaacaggtt atccagtaat ttcggatcct gaaccggaat gtaagtggta taacggttac
    39481 tatctttctt ggtaacgttg atttcacgtc cgttgatacg cgcttcacga acctggtcgt
    39541 tattgacctc ttgtaggaag gtagagtaat ccaccttacg gccattagac tcgctgggcc
    39601 caaagctctg gaatactgac atcagcacaa cggcaatgac cagccagagt attaggtttt
    39661 tcgccatgtc actcaaggga ttaacctctt attacaactg tgttaaaaac agcgtcagga
    39721 tactctatat ccagcatctt tcaaactttc gtctgaaatc tcccggttag ggtttacgcc
    39781 cggtcgctac aatatacact tcccgcgaac gtgcacgaga agagtccggc ttacgaactt
    39841 tgaccttcgt aaacagggag cgaatttccc ttagatactc atcgaaacct tcgccctgga
    39901 acaccttcac tacaaaactg ccacctggcg ctaatacatc acgacacatt tctaacgcca
    39961 gttccaccag atacatggca cgggggatat ccaccgccgg tgttccgctc atgtttggtg
    40021 ccatatcgga catgacaacc tggactttgc tgtcgccaac gcgctccagc agtgctttca
    40081 tcaccagttc atcacgaaaa tcgccctgaa gaaagtccac accaacgata ggatccatag
    40141 gtaaaagatc gcaagcgatg atgcggcctt tgccgccaat ttgggtgacc acatattgtg
    40201 accaaccacc cggagcagca ccaaggtcga caaccgtcat tcccggttta aagagtttgt
    40261 cactttgctg tatttcatca agtttaaacc aggcacggga acgtaacccc tttttctgtg
    40321 cctgttgaac atatttatcg ctaaagtgtt cctgaagcca gcggctggag ctggcagaac
    40381 gcttcttacc tgtcatttaa ctttcccatg gggataactc atcgtaacca attgcgtaaa
    40441 tttttacgcg cctatttggt gatatatggg agatggcggt agaatgaccc gttttcaatc
    40501 ccaacgtaag caaaaaatac gatgaatctg agtactaaac aaaaacagca cctgaaaggt
    40561 ctggcacatc cgctcaagcc agttgttctg cttggcagta atggtttgac cgaaggggtg
    40621 ctggccgaga ttgaacaagc gttagagcac catgaactca tcaaggtgaa aatcgccacc
    40681 gaagatcgcg aaactaaaac cttgatcgtg gaagctatcg tgcgcgaaac cggcgcctgt
    40741 aatgtacagg tcatcggtaa aacgctggtg ctttatcgcc caactaaaga acgtaaaatc
    40801 tcgctgccac gctaagatta tcctaaagtt acacacattt gctgtgtaaa acgaggggtt
    40861 ttccgcaggc aggagagcaa aatgccacgc tctgttcgtt gataaaaggc cgcatagcgg
    40921 cctttttcct ttctttacaa tacatcaaca tcttgagtat tgggtaattc ttacaggtat
    40981 tccaccttaa ttacttcaaa ttctacttcg ccgcccggcg ttttgatgac cacaacatca
    41041 tcttcttctt tgccgatcag gccacgagca ataggcgagt ttacagaaat caggttttgt
    41101 ttaaagtcag cttcgtcatc gccaacgatg cgataagtct gttcttcgtc agaatccaga
    41161 ttcagcaccg ttacggtagc accaaaaata acgcgcccat tgttgggcat tttggtgaca
    41221 tcaatcacct gcgcgttcga cagcttggct tcgatgtctt taatacggcc ttcgcagaaa
    41281 ccctgctgtt cacgagctgc gtggtattcg gcgttttctt tcaggtcgcc atgctcacgc
    41341 gcttccgcga tagcagcaat gatttcagga cggcgcacag atttcagaaa atccagctct
    41401 tcgcgtaatt tttcagcgcc gcgtaaggtc atcggaatag cttgcatttg ttatacctct
    41461 tgaatattcc tgatggggca agtcttcacc caccccggct gttcggcctg cccggcataa
    41521 ctcctcaccg ggaccagaag caaaaaaata ccgacccggg tacaagtccc aggtcagcta
    41581 caattcacat tttgatagtc attttaccct gaagttcccg aagggtcatc gtttacttta
    41641 tagggcgttg cgccgtagta tgacggctcg attccaggtt gttagcgcga gattatgcga
    41701 ttttccagat ttatcatcgg attgaccagc tgtatagcgt tcagtgttca ggccgcgaat
    41761 gttgatgagt acgttactca actccccgct ggtgccaacc ttgccctgat ggtgcaaaaa
    41821 gtcggcgcgt cggcccccgc tattgattac cacagtcagc agatggcgct gcccgccagt
    41881 acgcagaaag tgattactgc gctggcggcg ttgattcaac tcggccccga ttttcgtttc
    41941 accacgacgc ttgaaaccaa aggcaatgtg gaaaacggcg tacttaaggg tgacttagtg
    42001 gcgcgatttg gtgccgatcc gacgttaaaa cgtcaggata ttcgcaatat ggtcgcgact
    42061 ttgaaaaaat ctggcgtcaa ccaaatcgat ggcaatgtgt tgatagatac ctccattttc
    42121 gccagccacg ataaagcccc cggctggcca tggaatgaca tgacacaatg ctttagcgct
    42181 ccgcctgccg ctgccatagt tgaccgcaac tgtttctccg tctcgctcta cagtgcccca
    42241 aaacctggtg atatggcttt tatacgcgtg gcatcttatt accccgttac gatgttcagc
    42301 caggtacgca ccctcccccg aggttctgcc gaagcgcaat actgcgaact ggatgtcgta
    42361 ccaggcgacc tgaaccgctt tacgctgacg ggatgcctgc cacaacgttc tgagccgctc
    42421 ccgttggctt ttgccgtgca ggatggagcc agctatgccg gggcaattct gaaagatgag
    42481 ttaaaacagg caggtatcac ctggagcgga acactgctgc gccagactca ggttaacgaa
    42541 cctggaacgg tagttgccag taaacagtcg gccccgttgc acgatctgct taagattatg
    42601 ctgaaaaagt cggacaacat gatcgccgat acggttttcc gcatgatagg ccatgcgcgc
    42661 ttcaatgtgc ctggaacatg gcgggccggg tcggacgccg tgcgtcagat cctgcgccag
    42721 caagccggtg tcgatattgg aaacaccatt attgccgatg gttcagggct ttcgcggcat
    42781 aacctgattg cccccgccac catgatgcag gtgttgcaat acattgccca acacgacaat
    42841 gaacttaact ttatctccat gctgccgctg gcgggctatg acggctcttt gcagtaccgt
    42901 gcaggtctgc atcaggcggg cgtggatgga aaagtctcag cgaaaaccgg ttcgttgcag
    42961 ggggtatata acctggcggg attcattacc acagcgagcg ggcaacgaat ggcgtttgta
    43021 caatatcttt ctggctatgc agtagaaccg gcggatcagc gtaatcgccg tattccgtta
    43081 gtgcgttttg aaagccgttt gtataaagat atttatcaga acaattagtc aaaaagaaac
    43141 cccggcacat ggccggggtt ttgctgatta acgcttgtaa atgaactcaa cgccttcttc
    43201 gtcgtcttcg tcccagtcat catcccagtc ctcatcatcc tcttcagcaa tctcttcaag
    43261 ctgctggcga tgataatcat cccacatgaa ttcgactttc tctggctgtt tcgcttcttc
    43321 agcctgcacg accgggtttt caatgataaa ggtcatcaca tcccagcaga gatctttcac
    43381 gcccagtcca ctcgccgcag agatcagata atatttatct tcccagccca gcgcttcagc
    43441 aatcgctttc gctttctctt cagcttctgc cttatccagc agatcgatct tattgaacac
    43501 taaccaacgt ggtttcgccg ccagatcctg gctgtatttt tccagctcgc tgataataat
    43561 acgcgcgttt tcaaccggat cggtgccgtc aatcggatcg atatcgatga ggtgcaacag
    43621 gacgcggcaa cgttccaggt gcttcaggaa gcgaatgccc agacctgcgc cttccgcagc
    43681 gccttcaatc agtcctggaa tatcggcaac aacgaagctc ttttcgttgt ccattcgtac
    43741 cacacccaga cttggcacca gagtggtaaa cggataatcc gccactttcg gtttagccgc
    43801 cgataccgca cgaataaagg tcgatttacc cgcgtttggc atccccaaca taccgacgtc
    43861 agccagcagc atcagctcca gcagcagctc acgcttatcg cccggcgtac cgttagtttt
    43921 ctgccgtggt gtacggttaa ctgacgattt gaaacgggta ttgcccagac cgtgccagcc
    43981 gcccttagca accagcagac gctgaccgtg tttggtcata tcgcccatgg tttcaccagt
    44041 accctggtcg attacacgcg tacctaccgg cactttaatt gtgacgtctt taccgcgctt
    44101 accggtacag tcgcggcttg cgccattctg accgcgctct gcacggaaag atttttcaaa
    44161 acgataatcg ataagcgtgt tcaggttctc gtcggcttcc atccatacgt caccaccatc
    44221 accgccgtcg ccgccatccg ggccgccttt cggaatatac ttttcgcggc ggaagctcac
    44281 gcaaccatta ccgccatcac ctgcaacgac cagaatcgat gcttcatcaa caaacttcat
    44341 tttattctcc gtaaatcatt cgcctgcgcg gggttgcgat accaccgttg tatgcttacg
    44401 taatccgccc caaatacgat gaccaatggc ggaatacatc gcgcccgcaa ccacgacaaa
    44461 cgcaccgaga taacctaaaa ggtttaacat cggtctggcg aagaaatcgg gccaggccag
    44521 tgataaaaga tctgaaaaaa acagcgtaaa cagtggggtg agcgtgatga tcgcgctcac
    44581 ctgcgctgcc tgccagcgag ccatcgcttc cgccagggcg ccatatccta ccaaggtatt
    44641 cagtccgcaa aaaattaaac atgcgagctg ccagtggcta agctgcgcta tcactccagg
    44701 ctttgccaga gggaagagcg caattgtaca taaagtgtac agtaaaaaca ggatctgcgg
    44761 tgaggccagc cgacgcaata aaaccttttg cgccacgcca taactcaccc aaaccgtcgc
    44821 cgcaccgacc ccaaagataa ctccccaggt gtaatcggtg agctttgtaa atatctcgac
    44881 cagactggtg ttaaaaaaca tcaccaggcc gctcaggagc atcaatgccc ctacaacctg
    44941 agtgctgcgc attttctctt tcaggataaa tacgctggca accatcatgc caactggcga
    45001 gagttgccca atcacctgcg aagcggtcgg actcaggtat tgcaaggatg agctgaacag
    45061 gatgaagttc ccaaacagcc cggcggtcgc cactgccaac aaaatcaacc agcgtggctt
    45121 acgaaacacg cgtaatggcg gcaacctctt cttcaccgca agaatggcac caaggccaat
    45181 actcgccatc aagaaacggt aaaacacgat tgtcggaggt tccatcacct ccagcacctg
    45241 cttcattgcg attggcaacg ccccccagca aattgctgtg gtgagcgcca aaagaatgcc
    45301 aatgcctgcc tgctgcttca taccgtattc cctgcaaagc gcatttgcgg attaacgtcg
    45361 caccatgcgc atttttaccg gttatcgaat gtaaaaagcc ccgcaacgtg ttgcggggct
    45421 ttcatccgtt accgggacgc gaaaaactta ttcagcttcg atgctgataa atttacggtt
    45481 tttcgggcct ttaacttcga atttcacttt accgtctgct ttagcaaaca gagtgtggtc
    45541 acgaccgcaa cctacgttag cgccagcgtg gaatttggta ccacgttgac gaacgatgat
    45601 gctacccgcc agaacggatt cgccaccgaa acgcttaacg cccaggcgtt tagcttctga
    45661 atcgcgaccg ttacgtgtgg agccgccagc ctttttatgt gccatttgaa atctctcctc
    45721 aggtcttagg cgctgatgcc agtaattttc acatcagtga accactgacg atggccctgc
    45781 tgcttacgat agtgtttacg acgacgaaac ttaacgattt taactttctc gccacgaccg
    45841 tgagcaacaa cttcagcttt gattacgccg ccatcaacga aaggaacgcc gattttgact
    45901 tcttcaccgt ttgcgatcat cagcacttca gcgaactcaa cagtttcgcc agttgcgatg
    45961 tccagctttt ccaggcgaac ggtctgacct tcgcttactc ggtgttgttt accaccactt
    46021 tggaaaaccg cgtacatata aaactccgct tccgcgcaca ccttttcaat gattcagagt
    46081 gcgctataaa tattcacaat agggcgcgaa tattacgcaa aacgcacgcc tttgacaagt
    46141 gctacagtca atacacgaag aaaaaaaaca caacttgtac ggtaacgttt atctgtgcca
    46201 ttttttcagt acaatcactc tatattccta accataaacc ctaagttgcc tttgttcaca
    46261 gtaaggtaat cggggcgaaa agcccggctt ttgcgatgaa tttagaaaaa atcaatgagt
    46321 taaccgcgca agatatggcg ggtgttaatg cggcaatcct tgagcagctt aattccgacg
    46381 tccagctgat caatcagtta ggctattaca tcgtcagcgg cggcggtaaa cgtattcgtc
    46441 cgatgattgc tgtactggct gcacgagctg ttggctatga gggaaatgcg catgtcacca
    46501 ttgctgccct gatcgagttt atccacacgg cgactctgct gcacgacgac gttgtggatg
    46561 aatcagatat gcgcaggggt aaagctaccg ccaacgctgc atttggcaat gccgccagcg
    46621 tgctggtagg cgattttatt tatacccgcg cgttccagat gatgaccagc ctcggttcgc
    46681 tcaaagtgct ggaagtcatg tcagaagccg taaacgtcat cgcagaaggt gaagttctgc
    46741 aactgatgaa cgttaacgat ccggacatca ctgaagaaaa ctacatgcgc gttatctata
    46801 gcaaaaccgc gcgtctgttt gaggctgccg cgcagtgttc cgggattctg gctggctgta
    46861 cgccggaaga ggagaaaggc ctgcaggatt atgggcgcta tctcggcact gctttccagt
    46921 tgatcgacga tttactcgat tacaatgccg atggcgaaca gttaggtaaa aatgtcggcg
    46981 acgatctgaa cgaaggtaaa ccgacgctgc cgctgctgca tgcgatgcat catggcacac
    47041 cagaacaggc acagatgatc cgtaccgcca tcgaacaggg taacggtcgc catcttctgg
    47101 aaccggttct ggaagcaatg aacgcttgtg gatctcttga atggacgcgt cagcgtgccg
    47161 aggaagaagc agacaaagcc atcgcagcat tacaggtgct cccggacacc ccttggcgag
    47221 aagcactcat cggcctcgcg cacatcgctg ttcaacgcga tcgttaatcc cctcccttca
    47281 tcccgcgcag tgcgcgggat gattccagta aattccataa aaacactaat tcagcgctat
    47341 aacacctgaa aaaaagggcg atgaatcttt atatattctg aatattcaca ctctttacag
    47401 gaacttttta gagcaatagg ccatcaggag tatagtgatg cccgacagaa gaagtgttct
    47461 gaatgaaagc gactaactta aggagtgagg aaagatggaa agtaatttca ttgactggca
    47521 tcccgctgac atcattgcgg gtttgcgcaa gaagggaacg tcaatggcgg cggagtctcg
    47581 cagaaatggt ttgagttcct caacgctggc aaacgcatta tcgcgcccat ggccgaaagg
    47641 agagatgatt attgcgaaag ccctgggaac tgacccctgg gttatctggc catcacgcta
    47701 ccatgatccg cagacccatg agtttatcga cagaacgcag ttgatgcgta gctacactaa
    47761 accgaaaaaa tgagtggtct ggcggtagcc ccgcgaacgg ggctgccagc tctcagacga
    47821 ttactcgcct ttcacacgct caatatttgc acctaaagcg cgcagtttgt cttcaatgcg
    47881 ttcgtagcca cgatcgatgt gataaatacg atcaaccacc gtcgtccctt ccgcaataca
    47941 gccagccagc accaggcttg ctgatgcacg cagatcggtt gccataacct gtgcgccaga
    48001 aagtttttca acaccgtgac aaataacggt attgctttcg atttcggcat gcgcgcccat
    48061 acggctaagc tccggcacat gcataaagcg gttttcaaag accgtttcgg tgatgaatcc
    48121 ggtcccttct gccaccaggt tcaacagcgt gaactgggcc tgcatatcgg tcgggaatgc
    48181 cggatgcggc gcggtacgta cgttaacagc cttcggacgt ttgccatgca tatccaggct
    48241 aatccagtct tcgccgactt cgatgtccgc tccagcgtca cgcagtttcg ccagcacggc
    48301 gtcgagagta tctggctgcg cgttacggca gataattttg ccgcgagaaa tcgccgccgc
    48361 caccaggaaa gtaccggttt cgatacgatc cggtagcacg cgatagacac cgccgcctaa
    48421 acgttccaca ccttcgatga cgatacgatc ggtgccctga ccgctaattt tcgcacccag
    48481 cgtaatcagg aagttcgcgg tatcgacgat ttccggttca cgcgctgcgt tttcaataat
    48541 cgtggtacct tccgccaggg ttgcagcaca catgatggtc accgttgcgc caacgctgac
    48601 tttatccatc acgatatgtg cgcctttcag acgaccatcg acggaagctt taacgtaacc
    48661 ttcttccagt ttgatggtcg cgcctaattg ttcgaggcca gaaatgtgta gatcaaccgg
    48721 acgcgcaccg atcgtacaac cgccaggcag tgaaacttgc ccctgaccaa agcgcgctac
    48781 cagcggcccc agtgcccaga tagaagcacg catggtttta accagatcgt aaggtgcgca
    48841 gaatacatta acgtcgcggg catcaatatg cacagaacca ttacgttcta ctttcgcccc
    48901 cagctggctt agcagcttca ttgatgtatc gacgtctttc agttttggga cgttctggat
    48961 ctctaccggt tcttccgcca gtagagcggc aaaaaggata ggcagagcag catttttagc
    49021 gccggaaatt gtgacttcgc cctggagctt cgttggcccc tgaacacgaa atttatccat
    49081 ttagtttgtt ctcagttaac aattcatatc cgctaccggc taatcgccca tagctcaaaa
    49141 gccgttcagt ttgcgatcgc gcgcccactc cgcaggggta tacgctttga tcgacacagc
    49201 atgaatgcgg ttatccgcaa tatattccat cagcgggcca tagaccgtct gctgtttttt
    49261 aacccgactc atgccgtcaa acaactcacc cacggcaata acctgaaagt ggctgccatc
    49321 gccggaaacg tggacttcct ggagggagag agcgttcatc aacacgctct gaatttcatt
    49381 attttccatg ggatcttcaa tcatcagtta ataaaccagc gaaacatctt agagcaaagt
    49441 tgcgctggca taaataagca aaaagcctcg ctgataaatc agacaaggct cgacttgcag
    49501 gcaggtttgc cggacaggcg gttaacgcca tatccggcct gaaaaaattt aacgaggcag
    49561 aacatcagca ggcaaattat acaatttcgc gagggtatac actttgtcgt ttaccccctg
    49621 aagcgtcacg ttgttgccct gctttttcgc cagatcgata agatggagca gcagtgccag
    49681 tccacccgta tccacgcggg acacacggct aagatcgatg caggtaatcc ccttcaccgc
    49741 ttcctcacgc atttcccaaa gcggtagcag aacgtcctga tccagctctc cggataacgc
    49801 cagcgtgtca cccgtctgca tccagctcag tgactcgctc attatttttt ctcttccaga
    49861 gtgattttct gttgagaaat cgatttcagt tgcgcagtca ggccgtcgat acctttggta
    49921 cgcagcagcg ttccccactc gttttgtttg gtggtgatca tactgacgcc ttcagcaatc
    49981 atgtcgtaag cctgccagtt tcccgtctgg gagtttttac gccactggaa gtccagacgc
    50041 accggcggac ggccattcgg gtcaataatg gtaacgcgaa taggcacaat ggttttatcg
    50101 cccagcggct gttctggcgc aatctgatag gtttgaccgt gatacatcgc cagcgcctga
    50161 ccgtaagcct gcttcaggta ctcacggaaa gcggcaaagt aggcttcacg ttgagcaggg
    50221 gtcgcactct tgtaatactg gcccagcacc agcgcaccgg cgtatttcac ctgtacgtat
    50281 ggcagcagtt cctgatcaac aatggtacgc agataatccg ggttggcccg aatttgcggt
    50341 tgctcattct tcaggcgatc gaacgttttc tgcgccgcct cgtccatcag cttatacgga
    50401 ttggtctggt ctgccgcggt tgccgcactc agaggtgcaa tcaccagcaa agcgaccatc
    50461 attaaacgtt taaacatgcg tcggttctcc tgaaattatt tcgttgtacc cacaggttca
    50521 gtggtttcat tattacctgg cgcagcagct ggcgcatcgc cactattctt attgtcatcg
    50581 cctttactac cgtaaaggaa ctgaccaatg agatcttcca gcaccatcgc agacttagtg
    50641 tcctgaattg tatcgccatc cttcaggata gcagtcccca gttccgggtc ttcaaaaccg
    50701 acgtttaatg ccagatattg ttcccccagc aggccggaag tacgaatgct cagcgaactg
    50761 gtatctggaa tgtggttata acgttgttca atttccagcg ttacgcgcgg cagataggtt
    50821 ttcgggtcca gcgtaatatc cgccacccga cccacaacaa cgccaccaat actgaccgga
    50881 gagcgcgctt tcaggccgcc aatgttatcg aacgtcgcat aaagcgtgta ggtcggttca
    50941 gtacgtatgg acgtcacgtt cgccgccttc aggcaaacaa acagcgccgc cagcagtgct
    51001 gctaataaaa agatacccac ccaaatttca ttttttttcg tttgcatgaa ctcaattccc
    51061 aaacatcaat gcggtcagca caaaatccag ccccagaaca gccagagacg agtggacaac
    51121 ggtgcgagtg gttgcccggc taatcccggc agacgtcggg atggcgtcgt agccgttaaa
    51181 caacgaaatc cacgtcaccg tgatggcgaa caccacgctc ttaatcagac agttgaccag
    51241 atccatacgc cagtcgacgg cattttgcat tgccgaccag aagaacccgc tatcaatgcc
    51301 tttccaactg acgccgacca gagatccgcc ccagatcccc acggcgacga aaataaccgt
    51361 caacagtggt aatgaaataa ccccagccca gaaacgggga gaaataaccc gacgcagcgg
    51421 atccaccgcc atcatctcca tactggagag ttgctctgta gcgcgcatca ggccgatttc
    51481 tgcggttagc gccgaaccag cacgcccggc aaacaacaac gcggcaacca ccggccccag
    51541 ttcacgcagt agcgataacg ccaccagcat acccagactg gtttccgcac tataagtggt
    51601 cagaaccaga taaccttgca gccccaacac cattccgatg aacacgccag aaaccacaat
    51661 aatcagcatc gacaggacgc cgacattata gagctggcgc accagcagcg gcgcatgttt
    51721 gcgaaattcc ggtttgccga ccagcgcatt gaataacatt aacccggccc gcccgaacgt
    51781 tctcagggtt ttaatccctt tatgtccgag cgacgccagc gcatttaaca gcatgagtgg
    51841 cttaactccc tggtaaaaga tcagcgtgat aatcgccggc aggatagcgg aacggaacag
    51901 gcccgtcagc tatcccgtcc agaaactgac gtacgcgcgg atcaggattc gcctgcaacg
    51961 cctgggcact gccatgagcg ataatttttt tgtccgccag gatccaggcg tgatccgcaa
    52021 tacttaacac ttccggcaca tcgtgagaaa ccaccacaca agtcacgccc agcgcgctgt
    52081 tcagctcaga aatcagcttc accagtacgc ccatggtgat gggatcttgc ccaacaaaag
    52141 gttcatcaaa catgatgaga tccggctcca gcgcaatcgc acgcgccagc gctgcacgcc
    52201 gcgccatccc accagaaagt tcagaaggca ttagcttagc cgctccacgc agccccacgg
    52261 cctccagctt catcatcact gtactatgca acaatggcgc gggaagttgg gtatgttcgc
    52321 gcagtggata ggcgacgttg tcaaatacgt tcatatcagt gaacaacgcc ccggactgaa
    52381 ataacatgct catccgtttg cgcactgtat acaggcgcga acgagacatc gccggaatat
    52441 tctcaccatc gaaaaggatc tcaccatgat ctggtgcgat ttgcccgcca atcagacgga
    52501 gtagcgtcgt tttaccgatg cccgatggcc ccatgatcgc cgtgatcttc cctcgcggca
    52561 cggtcaggga aatattatcg aagatgcagc gattgccacg cgtaaaactg acatcgcgca
    52621 tatcgactaa attcgccaca gactgctcca taattcaccc ttcgtcttgc gttgattttc
    52681 taagcatggc gctcaattta accttgaacc caacatattt acagaatatt acccgccgtg
    52741 gttagcgaaa gctggcattt gttttacttt ttagccgcat aaagtcaaaa ttaagaatcc
    52801 gttacggctt tctgaaaatc ttcagcggac cggcgagtat acctgaagaa aggatgttag
    52861 atgcttttag ctacggcact gttaattgtt ggtttacttt tggtcgttta cagtgccgac
    52921 cgcctggttt ttgccgcgtc tattctttgc cgaacctttg gcatcccgcc gctgatcatc
    52981 ggcatgacgg tggtcagtat tggtacatcg ttaccagaag tcatcgtctc gcttgccgcg
    53041 tctctgcacg aacaacgcga tttagccgtt ggtacagccc tcggctcaaa cattatcaat
    53101 atattgctga tcctcggtct ggctgcgctg gttcgtcctt ttaccgtcca ttctgatgtt
    53161 ctacgccgtg aattaccctt aatgttgttg gtcagcgtgg tggccggttc cgtactctat
    53221 gacggacaac ttagtcgcag cgatggtatc tttctcctct ttctggctgt gctatggctg
    53281 ctgttcattg ttaaacttgc acgtcaggct gaacgtcagg ggactgacag cctgaccaga
    53341 gagcagcttg cagagctgcc gcgtgacggc ggattgcccg tcgcgttttt atggctcggc
    53401 attgcgctta tcatcatgcc agtggccacg cggatggtgg ttgataacgc cacggtgctg
    53461 gcgaattact ttgccatcag cgagttgacg atgggtctga cggcaattgc tatcggaacc
    53521 agcctgccgg aactggcaac cgcaatagcg ggggttcgca aaggtgaaaa cgacattgct
    53581 gtcggaaata tcattggcgc aaacattttt aatattgtca tcgtgttggg tttacccgcg
    53641 ctgataacgc caggagagat tgatccactg gcgtacagtc gtgactacag cgtgatgttg
    53701 ctggtgagca ttatttttgc gttgctgtgc tggcggcgct ccccacaacc gggccgtggt
    53761 gtaggggtat tattaactgg cggatttatc gtatggctgg cgatgttgta ctggttatcg
    53821 ccaatactcg ttgaataact ggaaacgcat tatgtcgcac gtagagttac aaccgggttt
    53881 tgactttcag caagcaggta aagaagtcct ggcgattgaa cgtgaatgcc tggcggagct
    53941 tgatcaatac atcaatcaga atttcacgct tgcctgtgaa aagatgttct ggtgtaaagg
    54001 gaaagttgtc gtcatgggga tggggaaatc ggggcacatc gggcgcaaaa tggccgcaac
    54061 gtttgccagc accggtacac cttcattttt cgtccatcct ggtgaagccg cgcatggtga
    54121 tttaggcatg gtcaccccac aggatgtggt gattgctatc tctaactcag gtgaatccag
    54181 cgaaatcacg gccttaattc cagtgcttaa gcgtcttcac gtaccgttaa tctgcatcac
    54241 cggtcgcccg gagagcagca tggcgcgcgc cgcagatgtg catctgtgtg ttaaagtagc
    54301 gaaagaagcc tgtccgttag ggctggcacc gaccagcagc accaccgcca cgctggttat
    54361 gggcgatgcc ctcgctgtcg cgctgttaaa agcacgcggc tttactgctg aagattttgc
    54421 gctctcacac ccaggcggcg cactgggtcg taaacttctg ctgcgcgtaa acgatattat
    54481 gcatacgggc gatgagatcc cgcatgttaa gaaaacggcc agtctgcgtg acgcattgct
    54541 ggaagttacc cgcaaaaatc ttggtatgac tgtcatttgc gatgacaata tgatgattga
    54601 aggcatcttt accgacggtg atttacgccg tgtcttcgat atgggcgtgg atgttcgtca
    54661 gttaagtatt gccgatgtga tgacgccggg gggaatacgc gtgcgccctg gcattctggc
    54721 cgttgaggca ctgaacttaa tgcagtcccg ccatatcacc tccgtgatgg ttgccgatgg
    54781 cgaccattta ctcggtgtgt tacatatgca tgatttactg cgtgcaggcg tagtgtaaag
    54841 attcaaggat aaacaacaat gagcaaagca ggtgcgtcgc ttgcgacctg ttacggccct
    54901 gtcagcgccg acgttatggc aaaagcagag aacattcgtc tgctgatcct cgacgttgat
    54961 ggcgtactgt cagatggcct gatttatatg ggcaataacg gcgaagagct gaaagcgttc
    55021 aatgttcgtg acggttatgg cattcgttgt gcgctcacct ctgatattga agtcgctatc
    55081 attaccgggc gaaaggctaa actggtagaa gatcgttgtg ccacattggg gatcactcac
    55141 ttgtatcagg ggcagtcaaa taaactgatc gcctttagcg atctgctgga aaaattggcg
    55201 attgccccgg aaaatgtggc ttatgtcggc gatgatctca tcgactggcc ggtaatggaa
    55261 aaagtgggtt taagcgtcgc cgtggccgat gcgcatccac tgttgatccc gcgcgccgat
    55321 tacgtgacgc gcattgctgg cggtcgtggc gcagtgcgcg aagtttgcga cttattactc
    55381 ctggcgcagg gcaaactgga tgaagccaaa gggcaatcga tatgagtaaa gccagacgtt
    55441 gggttatcat tgtgctatca ctggcggttc tggtgatgat cggcattaat atggccgaaa
    55501 aagacgatac cgcccaggtg gtcgtcaaca acaatgatcc cacctataaa agcgagcata
    55561 cggacacgct cgtctataac ccagaagggg cactaagcta tcgattgatt gctcaacacg
    55621 ttgaatatta ttccgatcag gccgtttcgt ggtttacgca gccggtactt accacgtttg
    55681 ataaggataa aatcccgaca tggtccgtaa aagcagataa agccaagctg accaatgacc
    55741 ggatgctcta tttatatgga cacgttgaag tcaacgcact tgtgccggac tctcaacttc
    55801 gcagaatcac gacggataac gcgcagatca atctggtgac gcaggatgtt acctctgaag
    55861 acctcgtcac gttatacgga acaacattta actccagcgg tctgaaaatg cgcggcaact
    55921 tacgcagcaa gaacgccgag ctgattgaaa aggttagaac atcctatgaa attcaaaaca
    55981 aacaaactca gccttaatct tgtgcttgcc agctcacttc tggccgccag cattccggca
    56041 tttgccgtaa ccggagacac tgatcagccg atccacattg aatcggacca gcaatctctt
    56101 gatatgcaag gcaacgtggt tacctttacc ggtaatgtca tcgtcaccca gggcaccatc
    56161 aaaattaatg ccgacaaagt ggtcgttacc cgtccgggcg gcgaacaagg taaagaagtg
    56221 attgacggct acggtaaacc ggcaacgttc taccagatgc aggataacgg taaaccggtt
    56281 gaaggtcacg cttcccagat gcactacgaa ctggcaaaag attttgtcgt tctgacgggt
    56341 aatgcttatc tgcagcaggt tgatagcaac attaagggcg ataagatcac ttacctggta
    56401 aaagagcaga aaatgcaggc tttcagcgac aaaggcaagc gcgtaaccac cgttctggtg
    56461 ccgtcgcagc tgcaggacaa aaacaacaaa ggccagaccc cggcacagaa gaagggtaat
    56521 taattcgtta tggcaacatt aactgcaaag aaccttgcaa aagcctataa aggccgtcgc
    56581 gtggtagaag acgtcagcct gaccgtcaac tccggggaaa ttgtcggtct gctggggcca
    56641 aacggtgccg gtaagaccac cactttctac atggttgtag gcattgtgcc gcgcgatgcg
    56701 ggcaacatca ttattgatga tgacgatatc agtctgctgc ctctgcatgc acgcgcgcgc
    56761 cgcggaatcg gctatctgcc acaggaagcc tccattttcc gtcgcctcag cgtttacgat
    56821 aacctgatgg cggtactgca aattcgtgac gacttgtctg ctgaacaacg tgaagaccgc
    56881 gcgaacgagc tgatggaaga gtttcacatt gagcacctgc gtgacagcat ggggcagtca
    56941 ctctccgggg gtgaacgtcg ccgtgtagaa attgcccgcg cactggctgc gaatccgaaa
    57001 tttattctgc tcgacgaacc gtttgccggg gttgacccga tctcggttat cgacattaaa
    57061 cgcatcattg agcacctgcg cgacagcggc ctgggcgtgc tgatcactga ccacaacgtg
    57121 cgtgaaacac tggcggtttg tgaacgcgct tatatcgtca gtcaggggca tttgatcgcc
    57181 cacggcacgc ctacagaaat cttacaagac gaacacgtta agcgtgtata ccttggggaa
    57241 gacttcagac tctgataggg tagaagtttg cgacgtttta gcaggagagt acgattctga
    57301 acatgaagca aggtttgcaa ctcaggctta gccaacaact ggcgatgacg ccacagctcc
    57361 aacaggcaat tcgtctgttg cagttgtcga cgctggaact tcagcaggag ctacagcagg
    57421 cgctggagag taatccgctg cttgagcaaa tcgacactca tgaagaaatc gacacccgcg
    57481 aaacgcaaga cagtgaaacg ctggacaccg ccgacgcgct cgaacaaaaa gagatgccgg
    57541 aagagctgcc gctcgatgcc agttgggaca ccatttacac cgctggtaca ccatccggca
    57601 ccagcggtga ctacattgac gatgagctgc cggtctatca gggcgaaacg acgcagacct
    57661 tgcaggatta cctgatgtgg caggtcgagc tgacaccgtt ttccgacact gaccgcgcta
    57721 ttgctacctc tatcgtcgat gccgttgatg acaccggtta tctgactgtc ccgctggaag
    57781 atattctcga aagtatgggc gatgaagaga tcgacatcga cgaggttgaa gccgtcctta
    57841 agcggatcca acggtttgat ccggtcggtg tagcggcaaa agatctgcgt gactgcctgc
    57901 taatccaact ctcccaattc gataaaacca cgccgtggct ggaagaggcc agactgatca
    57961 ttagcgatca tctcgatctg ttagccaatc acgacttccg cactttaatg cgcgtcacgc
    58021 gtctgaaaga agatgtgctg aaagaagccg tcaatctgat ccagtcgctc gatccgcgcc
    58081 ccgggcagtc gatccagact ggcgaacctg agtatgtcat tccagatgtg ctggtgcgta
    58141 agcataacgg tcactggacg gtagaactca acagtgacag cattccgcgt ctgcaaatca
    58201 accagcacta cgcctcgatg tgcaataacg cgcgcaacga tggtgacagc cagtttatcc
    58261 gcagcaatct gcaggatgcc aaatggttga tcaagagtct ggaaagccgt aacgatacgc
    58321 tactgcgcgt gagtcgctgt atcgttgaac agcagcaagc cttctttgag caaggtgaag
    58381 aatatatgaa accgatggta ctggccgata tcgcccaggc tgtcgaaatg catgaatcga
    58441 cgatatctcg cgtgaccacg caaaaatacc tgcatagtcc acgaggcatt tttgaactga
    58501 agtatttctt ttccagtcac gtcaataccg agggcggcgg cgaagcttcc tccacggcga
    58561 ttcgtgcgct ggtgaagaaa ttaatcgcgg cggaaaaccc agcgaaaccg ttgagcgaca
    58621 gcaagttaac ctctttgctg tcggaacaag gtatcatggt ggcacgccgc actgttgcga
    58681 agtaccgaga gtctttatcc attccgccgt caaaccagcg taaacagctc gtttgaccca
    58741 accgataagg aagacactat gcagctcaac attaccggaa ataacgtcga gatcaccgag
    58801 gcactgcgcg aatttgttac agccaaattt gccaaacttg agcaatattt tgaccgaatc
    58861 aaccaggtct atgttgttct gaaagtggag aaagtcaccc acacctcaga tgcaacactg
    58921 catgtaaacg gtggtgaaat tcatgccagc gcggaaggtc aggacatgta cgctgccatt
    58981 gatggcttaa ttgataagct ggcacgccag ttgaccaaac ataaagataa actgaaacaa
    59041 cactaattgt ccgggcaatt agcatgtgca tggcggcctg ttgtgcggca caacgggcca
    59101 tttgtacggt taatgctccg agcctgttcc actgtttgag tgggcaggtt cttaggtgaa
    59161 attatgacaa ataatgatac aactctacag cttagcagtg ttcttaacag ggaatgtacg
    59221 cgaagccgcg tccactgtca gagcaaaaaa cgcgccctgg aaatcatcag cgagctggcg
    59281 gcgaaacaac ttagcctgcc gcctcaggtg gtttttgaag ctatcctgac gcgggaaaaa
    59341 atgggcagta ccggtatcgg caatggtatt gcaattccgc atggcaaact ggaagaagat
    59401 actctgcgtg ccgttggcgt tttcgttcag ctcgaaacgc ctatagcttt cgatgccatc
    59461 gacaaccaac cggtggactt actttttgcc ctgctggtgc cggcagacca aactaaaacg
    59521 cacctgcata ctctgtcgct ggtggcgaaa cgtctggcgg acaaaaccat ctgccgccgt
    59581 ttacgcgcag cccagagcga tgaagagctg tatcaaatca ttacggatac cgaaggtact
    59641 ccggatgaag cgtagttatt cggtaatgtc tcttttagac gttgtgagga gaaacagtac
    59701 atggtactga tgatcgtcag cggacgttca ggttcaggta aatctgtcgc cctgcgtgcg
    59761 ctggaagata tgggttttta ctgcgtggat aaccttcccg tagtgttgtt acccgatctg
    59821 gctcgaaccc tggccgatcg cgagatttct gccgccgtca gcattgatgt tcgtaatatg
    59881 ccggagtcac cagaaatatt cgaacaggcg atgagtaacc tgcctgacgc tttctcaccg
    59941 caactactgt tcctggatgc cgaccgtaat accttaattc gtcgttacag tgacacgcgc
    60001 cgactgcatc cgctttccag caaaaacctg tcgctggaaa gtgctatcga caaagaaagc
    60061 gatttgctgg agcctctgcg ttcgcgagcg gatctgattg tcgatacctc agaaatgtcc
    60121 gttcacgagc tggctgaaat gctgcgtacc cgtctgctgg gtaaacgtga acgcgaactg
    60181 acaatggtct ttgagtcttt cggcttcaaa cacggtatcc ctatcgatgc agattacgtc
    60241 tttgacgtgc gcttcttgcc gaacccgcac tgggatccga aactgcgtcc aatgacaggt
    60301 cttgataagc ccgtcgccgc gttcctcgac cgccacacag aagtacacaa ttttatctac
    60361 cagacgcgaa gctatcttga gctatggtta cctatgctgg aaaccaacaa ccgtagctac
    60421 ctgacggtcg ccattggttg taccggcggg aagcaccgtt cggtgtatat tgcagagcaa
    60481 ctggcagact acttccgctc gcgcggtaaa aacgtccagt cacgccatcg tacgctggaa
    60541 aaacgtaaac catgaccgtc aagcaaactg ttgaaatcac aaacaagctg ggcatgcatg
    60601 cccggcctgc aatgaagctg tttgaattaa tgcagggttt tgacgctgaa gtgctgttac
    60661 gtaatgacga aggcaccgag gccgaagcca acagcgttat tgcgctgctg atgttagatt
    60721 ctgccaaagg acggcagatt gaagttgaag cgaccggccc acaggaagag gaagcactgg
    60781 cagccgttat cgccctcttt aactctggtt ttgatgaaga ttaatcttca tcactttgac
    60841 atacaactcc cttcaaactc cccccgacaa taagaaaatc acgtactgaa atcgttctca
    60901 atcaacgtca tttgtacatt ttgtgcgctt ttcactttca gaagaacctt aagaaaacct
    60961 taagaggcat tgtttaggtt ttgtttaagt taatcgacca tactggagat cgtcagaaaa
    61021 tatttccagg agatggcatg attcgcttat ctgaacaaaa tcccctgggc accggaagac
    61081 atcggaaatg ttatgcgcat ccggaagatg cccaacgctg tatcaagatt gtctaccatc
    61141 gtggcgatgg tggcgataaa gagatccgcc gtgagttaaa gtactacgcg catcttggtc
    61201 gccggttaaa agactggagt ggaataccgc gctatcacgg taccgttgag acggattgcg
    61261 gaaccggtta tgtctacgat gtgattgcag attttgacgg caaaccctcc attacgctga
    61321 ccgaatttgc tgagcaatgc cgttacgaag aagacattgc acaactgcgc cagctactga
    61381 aacagctgaa gcgttatttg caggataacc gtatcgtgac gatgtcgctg aagccgcaga
    61441 atatcctctg tcaccgcatc agtgagtcag aggttatccc ggtcgtctgc gacaacattg
    61501 gtgaaagcac attgattcca ctggcgacct ggtcgaagtg gtgttgtctg cgtaagcaag
    61561 agcggttatg gaaacgattt atcgcccaac cggcgctggc tattgccctg cagaaagatc
    61621 tgcaacctcg tgagagcaaa acgctggccc tcacttcgcg cgaagcttaa tccagctggt
    61681 gttgttgcat aaacggctca ccgcctaact gatacatctg ccgtaaaatc cacgcctgac
    61741 ggctacgcac gtagcccgat ggcgaggaga ctttgaaacg aagtggatta ggtaatacag
    61801 ctgccagtaa tgcagcttcc gaccgggtaa gtttgctcgc gggtttgtgg aaataacgtt
    61861 gtgccgcagc ttcgacgcca aacacaccgt cgccaaattc ggcgatattc aggtaaaccg
    61921 tcaggatacg ctttttgctc cagactgttt ctatccccag cgttaatcca gcttccagcc
    61981 cttttcgtac ccagctacgc ccatcccata aaaagagatt tttggccgtc tgctgagaaa
    62041 tcgttgaagc accgcgaata cggttttcat tgcgctcgtt gtgcgccagg gctttctcaa
    62101 tggaagcgac atcaaagccc cagtgctcag gaaatttctg atcttctgcg gcaatcaccg
    62161 ccagtcccat ccacggcgag atttgatcca tactgaccca gtcagaatgt gccacgtaac
    62221 gaaaattgcc atgcagccag gcgctgacct gtcgctcgac cattaccgct gagaagggaa
    62281 caggcgcaac actaaacaac gcgatgcccc cgccccagaa aacggcgagg acaaccatta
    62341 accgcaaaag aaaacggcga acgaaactaa acaccgttaa gcggctttta ctcattcagc
    62401 cagaaccagc acgcgggaaa ccagcttatc aatgccgctc gccgcttctg caatgttctg
    62461 cgccagcata tatgctgggg tggtgacaat cttattgtct tcatcaacca cgatatcatc
    62521 gacaggacag ggcacatgct ccgcgcccat ctcttccagc acttctgcgg tatcgatatc
    62581 agtaccgatg gtcaaacgca gcgggaaatc gaaaattttc ggcagcatcg ctggggcaat
    62641 acacataaaa ccaagcggtt ttccggcctg atgcatcgct tgtgccagcg cctttaattc
    62701 acggtcaacg gtgcattcgc taccaagagt ggcaaaattg cttaaattct tcgccgcgcc
    62761 aaaccccccc ggcacaatca acgcatccag ttcagcggca tcggcctggg ccagaggacg
    62821 gatttcaccg cgcgttattc gtgccgcttc aatcagcacg ttgcgcgttt ccgtcatcgc
    62881 ttcgccagtt aaatggttga taacatcaac ctgctgctta tccggtgcaa agcagaccgc
    62941 ctgcgcaccg ctgcgtgaaa tagctaacaa cgtcaacacc gcttcatgaa tttcagaacc
    63001 gtcatagacg ccgcatccgc tcagaattac gccaattttc ttcattgtga tcatcctttc
    63061 gcaattgact gaaacacatt aattttttta ataaaaatgg tacgcatcac acatttaact
    63121 gattcatgta acaaatcatt taagttttgc tatcttaact gcgtgcggcc tgaaaaacag
    63181 tgctgtgccc ttgtaactca tcataataat ttacggcgca gccaagattt ccctggtgtt
    63241 ggcgcagtat tcgcgcaccc cggtctagcc ggggtcattt tttagtggct tttgccaccc
    63301 acgctttcag cacttctacg tcgtgacgcc actcttcttt catctcttca atccattcac
    63361 cgacgttatc ttcccaggca ggaaggtccg gagactgaat ttgctgaccc agctgttgca
    63421 gatggcgtaa ccccactgac cccgccgcac ctttaatttt atgtccttcc tctacaatgc
    63481 cttttttatc ctgcgccgtc agattcgact ccagcacgct gacatagccc ggcatcatct
    63541 tctcaaacac cgctaacccg tcggtgatca gcttcggtcc tacaagttcg agatactgtt
    63601 ccagcatggg aatatcgagc aatgcttctg atttactgtt ctcttctgtc gtcaccgtac
    63661 tctcctcatc atcctgggta tcccagaatt tcttgatcat cgcggttagc gccggaaccg
    63721 aaagcggctt actcagcaca tcatccattc cagcattgag gtactcttgt ttgtctttca
    63781 gcacgttagc ggttaaggcc accagcggcg gtaaatcctc gcgcggataa cgcttcgtca
    63841 gttcacgaga gatatccagc ccggtcatat ctggcaactg aatatccagc aacaccaggt
    63901 cgtattcgcc cggtttaaac atctccagcg ccgccttgcc ggtcatggcg acatcaacgc
    63961 tgttacctaa tttttccagc acagaacgcg caacaatcac gttcagttca atgtcttcca
    64021 ccagcagcac attcagcgcc ggtaaaggca tatcgtcttc atcaaacgca tcatcgacct
    64081 cttctgctac cgacggtgcg tggatcgtca acgtaaaggt tgaacctttg ccctgttcgc
    64141 tggtaaccgt aatatcgccg cccatatttt tcgccagacg acgagaaacg gccagaccaa
    64201 taccggtgcc ggtggcaggt ttaccgccat gactgtcttt cacctggtaa tacatggcga
    64261 aaattttatc cagctcatcc tgcggaatgc cgataccaga gtcttccact tcaaaatgca
    64321 gcatatcgcc ttcatcgtag cgcacgcgca cggtaacctg gccttgctgg gtgaatttga
    64381 cggcgttact gatgaggttc cacaggatct gccgtaaacg cgtcccgtcg gtaatgacct
    64441 gatgcggtaa tggcagcgtc ggctccaggt taaagcgcaa tcctttttgt tgcgcctgca
    64501 atgcggagag attttccaga tcggcaagga agctggtgaa atcaaccggt tgattatcaa
    64561 gctggacctt gcgccgttcc atcttatcca tgtcgataat atcgttaaag atattcccca
    64621 gcgtgacggc cgaaacatgg atggtcttga gatatttttc ctgctcggcg gtgagttcgg
    64681 tatccagcag aatacggctc aggccgacga taccgttcag cggtgtacgc aattcgtgac
    64741 tgatggtgga gataaacgtc gttttgtcgc ggctggcccg ttcaagcgca tcctgatacc
    64801 gcttacgctc ggtaatgtcg cgaccaaagc ccatcaaacc gtgacgttta cccacgcggt
    64861 cgtagtacgg cactttacgg atttcaaagc aggctttgcg cccgtccggg taatccagcc
    64921 actgttcata ggtcagtgac acattatgac ggaacacttt ttcatcggtt tcaatgactt
    64981 ttgcggcggc ttccggtgag taaacatcag caggtttcag gtgaaccagt tgtttttcgc
    65041 tttttccggt cagcagctcc atcgcgcggt tacagccgga aaactcttta tcttcgttac
    65101 gataaaaaac caggtcgggt gaagcatcaa ggaaggaacg taagaatgag gattgctgct
    65161 cgagctgaat ttgtgtctct tcgcgctctt tgatttcaat tttcagttgg ccgaaggttt
    65221 cctgcagttc tgcttccgct ttttcacgaa cggcgatttc ctgatttagc tgggcaatat
    65281 tatcttttaa ctgaacgttg aggctcaaat cgcgctcgcg catctcctcc agtttttgca
    65341 ccagccgtga cagacgttgt cgtgactcct ccagttgctc gacgaccacc gatagaaagt
    65401 agaccgccca cggcgtaatc agcaaaccaa agaagataga acgaataaca tcaatgcttt
    65461 cgacctgacc atgcagcacc atggttaccg ccatttgtac cacaatggca agaacgacga
    65521 gggccagcgc cagcaacatt gagaagcgca ccagacctaa cttcatcatc aggtcaacat
    65581 aatactgcgc cagcagacga atttgcttca ttagggaatt ccttcacgac aacctgcccc
    65641 aataataccc aattctgaca gttacgttat aaattgtgtg agaaatgcgg aattcccctc
    65701 aacgacctac tccgttggag gtatccacgg acgttccagc gccgatccct gtacgccatg
    65761 ttcattcaga tagcgatcca gctcgaccat tcccgtccat cgattttcgc accacagagg
    65821 agcaagcagc gtcgggcgac gggcgctggc ggaaatgcgg tggtagatca cttccggtgg
    65881 cgtatggcga atcatttctc cggcagtgag cgtgtaatcc tccagttcaa taccgttcaa
    65941 acgccccgct tcccaggctt tcgccataat gctgcctttc acaatatgca gcggatgcag
    66001 ctttatgccg tccacgccgg tttcaaccac tcgctccagc gtttgcaaac attcagcctg
    66061 cccttcgcca ggcaggccga caattaagtg ggaacacacc ttcagcccgc gctgacgtgc
    66121 cagctgggtt gtacgctgat aacaggcaaa atcatggccg cggttgatgc gatgcagtgt
    66181 tttgtcgtgg gcggtttgta gccccagctc cagccacact tcgtaaccct ggtccttata
    66241 ttcgcaaagc agatccagca ccgcatccgg cacgcagtcc gggcgggtac caacacacaa
    66301 accgacaata ttggcctggc tcaccgcctg ctgatacata gaacgcagca cctgaacttc
    66361 cgcaaaggtg ctggtatacg cctgaaagta ggccagatag cgtttagcgc ggttaactaa
    66421 attcgcctgg tgcgccagtt gctcggcaat ggaacgatgc tgctgcgctt catcggcaaa
    66481 cgaggcaaca ttacagaatg tgcagccacc acgcccgatg gtaccgtcac ggttagggca
    66541 gctaaaaccg ccgtgtagcg tcagcttatg caccttttgc ccataacgac gggtgagatc
    66601 accaccaaac atattgacta atttctgtaa ctgcataatc tgatagaccg cgccttgaaa
    66661 agaggccaaa gcctgccatt tttagctcaa ttcggcgatg acctggatca atcgtcccgg
    66721 cctgctttta tcaactgcat aatcaatcaa aattaccgaa atttcatgca taatcacata
    66781 aatcactttt gcttatcttg tgtcagattt ttttatctcc ttatggattt taggcaaaaa
    66841 cagtagcatg aaacgtcatt accaattaag gcagtataaa atgctggttt tctcgtcagt
    66901 tcaaggcagg ataagggtta acacgccttt atgacagtca ggaattgact gtttctctaa
    66961 cgacttccct tttagcctta aagataaaat ccattttaat ttcagtaatt taataaagaa
    67021 ttt
//
LOCUS       DAPHDW010000006        65394 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.006, whole genome shotgun sequence.
ACCESSION   DAPHDW010000006 DAPHDW010000000
VERSION     DAPHDW010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 65394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65394
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.006"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..574
                     /locus_tag="RH057_000359"
     CDS             32..574
                     /locus_tag="RH057_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3126781.1"
                     /translation="MKKSVLTAFITVVCATSSVMAADDNAITDGKVTFNGKVIAPACT
                     LVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALEGCDVTVTKNATFTFSGTADG
                     VQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETSNIQLADSAEQTVTFKVDYIA
                     TGKATSGNVNAVTNFHINYY"
     gene            657..1358
                     /locus_tag="RH057_000360"
     CDS             657..1358
                     /locus_tag="RH057_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3126782.1"
                     /translation="MKTCITKGIVTVSLTAILLSCSSAWAAGKGGIGLAATRLVYSEG
                     EEQISLGVRNTSPDVPYLIQSWVMTPDNKKSADFIITPPLFVLNPANENLLRIMYIGA
                     PLAKDRETLFFTSVRAVPSTTKREEGNTLKIATQSVIKLFWRPKGLAYPLGEAPAKLR
                     CTSSADMVTVSNPTPYFITLTDLKIGGKVVKNQMISPFDKYQFSLPKGAKNSSVTYRT
                     INDYGAETPQLNCKS"
     gene            1383..3982
                     /locus_tag="RH057_000361"
                     /pseudo
     CDS             1383..3982
                     /locus_tag="RH057_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
     gene            3973..5043
                     /locus_tag="RH057_000362"
     CDS             3973..5043
                     /locus_tag="RH057_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3126783.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYSGQCHTTQGNPYI
                     GVNFGVKTLEEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTSVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATTDPSYPQAIKTSRPGVGVVVTDSQNNIISPAGGTLPLSIPDDADSIARMNVYPVS
                     TTGVPPETGRFEATATVRINFD"
     gene            5055..5597
                     /locus_tag="RH057_000363"
     CDS             5055..5597
                     /locus_tag="RH057_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3126784.1"
                     /translation="MKKKTIFQCVILFFSILNIHVGMAGPEQVSMHIYGNVVDQGCDV
                     ATKSALQNIHIGDFNISDFQAANTVSTAADLNIDITGCAAGITGADVLFSGEADTLAP
                     TLLKLTDTGGSGGMATGIAVQILDAQSQQEIPLNQVQPLTPLKAGDNTLKYQLRYKST
                     KAGATGGNATAVLYFDLVYQ"
     gene            5605..6120
                     /locus_tag="RH057_000364"
     CDS             5605..6120
                     /locus_tag="RH057_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3126785.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            6086..6823
                     /locus_tag="RH057_000365"
     CDS             6086..6823
                     /locus_tag="RH057_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV3126786.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELRAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPAPLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQLSVNGKALSNAGVVPPKSQRQTSWCQ
                     AIAPCHVAWRAINDYGGLSAKKEQNLP"
     gene            6934..7944
                     /gene="pyrD"
                     /locus_tag="RH057_000366"
     CDS             6934..7944
                     /gene="pyrD"
                     /locus_tag="RH057_000366"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HDV3126787.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEEELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            8118..8660
                     /gene="zapC"
                     /locus_tag="RH057_000367"
     CDS             8118..8660
                     /gene="zapC"
                     /locus_tag="RH057_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HDV3126788.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKEQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(8657..9766)
                     /gene="ycbX"
                     /locus_tag="RH057_000368"
     CDS             complement(8657..9766)
                     /gene="ycbX"
                     /locus_tag="RH057_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HDV3126789.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLAR"
     gene            10010..12118
                     /gene="rlmKL"
                     /locus_tag="RH057_000369"
     CDS             10010..12118
                     /gene="rlmKL"
                     /locus_tag="RH057_000369"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HDV3126790.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQPGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERQKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTGRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNHQIHNCWLITAA"
     gene            12130..14037
                     /locus_tag="RH057_000370"
     CDS             12130..14037
                     /locus_tag="RH057_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3126791.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDRKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRALSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKTEEAAAAKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            14167..15420
                     /gene="pqiA"
                     /locus_tag="RH057_000371"
     CDS             14167..15420
                     /gene="pqiA"
                     /locus_tag="RH057_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HDV3126792.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            15425..17065
                     /gene="pqiB"
                     /locus_tag="RH057_000372"
     CDS             15425..17065
                     /gene="pqiB"
                     /locus_tag="RH057_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HDV3126793.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTNNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            17062..17625
                     /gene="pqiC"
                     /locus_tag="RH057_000373"
     CDS             17062..17625
                     /gene="pqiC"
                     /locus_tag="RH057_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HDV3126794.1"
                     /translation="MKKWLVTIAALWLVGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            17881..18048
                     /gene="rmf"
                     /locus_tag="RH057_000374"
     CDS             17881..18048
                     /gene="rmf"
                     /locus_tag="RH057_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HDV3126795.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLNQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(18118..18636)
                     /gene="fabA"
                     /locus_tag="RH057_000375"
     CDS             complement(18118..18636)
                     /gene="fabA"
                     /locus_tag="RH057_000375"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HDV3126796.1"
                     /translation="MVDKRESYTKEDLLASGRGELFGAKGPQLPAPNMLMMDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDGRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(18705..20465)
                     /locus_tag="RH057_000376"
     CDS             complement(18705..20465)
                     /locus_tag="RH057_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HDV3126797.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIANAARTLQSDAGQLVGGHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVTQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRAQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            20651..21103
                     /gene="matP"
                     /locus_tag="RH057_000377"
     CDS             20651..21103
                     /gene="matP"
                     /locus_tag="RH057_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HDV3126798.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(21179..22219)
                     /gene="ompA"
                     /locus_tag="RH057_000378"
     CDS             complement(21179..22219)
                     /gene="ompA"
                     /locus_tag="RH057_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HDV3126799.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     INNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGSVENGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKSNVYGKNHDTGVSPVFAGGVEYAITPEIATRL
                     EYQWTNNIGDAHTIGTRPDNGMLSLGVSYRFGQGEAAPVVAPAPAPAPEVQTKHFTLK
                     SDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLSER
                     RAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEIEV
                     KGIKDVVTQPQA"
     gene            complement(22576..23085)
                     /gene="sulA"
                     /locus_tag="RH057_000379"
     CDS             complement(22576..23085)
                     /gene="sulA"
                     /locus_tag="RH057_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HDV3126800.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLADDLTEEEHAELVDAANEGNAMGFIMRPVSASSHATRQLSG
                     LKIHSNLYH"
     gene            23304..23933
                     /gene="sxy"
                     /locus_tag="RH057_000380"
     CDS             23304..23933
                     /gene="sxy"
                     /locus_tag="RH057_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HDV3126801.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEVGIKDVRALRILGAKMCWLRLRQQN
                     SLVTEKILFMLEGAIIGIHEAALPVARRQELAEWADSLTPKQEFPAELE"
     gene            complement(23896..26058)
                     /gene="yccS"
                     /locus_tag="RH057_000381"
     CDS             complement(23896..26058)
                     /gene="yccS"
                     /locus_tag="RH057_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309071.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HDV3126802.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGLLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQPYQHDPHFERAFTHIDAALERMRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQALPHNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAIRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWQFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDEAVCYVDDALHHQPADEERVNEALASLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQITQVPQETPVSA"
     gene            complement(26068..26514)
                     /locus_tag="RH057_000382"
     CDS             complement(26068..26514)
                     /locus_tag="RH057_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HDV3126803.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIMLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            26637..28691
                     /gene="helD"
                     /locus_tag="RH057_000383"
     CDS             26637..28691
                     /gene="helD"
                     /locus_tag="RH057_000383"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HDV3126804.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWQRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQAMSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDEKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSDGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(28723..29181)
                     /gene="mgsA"
                     /locus_tag="RH057_000384"
     CDS             complement(28723..29181)
                     /gene="mgsA"
                     /locus_tag="RH057_000384"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HDV3126805.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(29277..29939)
                     /locus_tag="RH057_000385"
     CDS             complement(29277..29939)
                     /locus_tag="RH057_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HDV3126806.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            30112..30525
                     /locus_tag="RH057_000386"
     CDS             30112..30525
                     /locus_tag="RH057_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HDV3126807.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(30570..30887)
                     /gene="hspQ"
                     /locus_tag="RH057_000387"
     CDS             complement(30570..30887)
                     /gene="hspQ"
                     /locus_tag="RH057_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HDV3126808.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(30945..32135)
                     /gene="rlmI"
                     /locus_tag="RH057_000388"
     CDS             complement(30945..32135)
                     /gene="rlmI"
                     /locus_tag="RH057_000388"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HDV3126809.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECSIYDRSDVA
                     VRKKEGMELTQGPVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            32230..32508
                     /gene="yccX"
                     /locus_tag="RH057_000389"
     CDS             32230..32508
                     /gene="yccX"
                     /locus_tag="RH057_000389"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HDV3126810.1"
                     /translation="MSKVCIIAWVYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELADFRIH"
     gene            complement(32505..32834)
                     /gene="tusE"
                     /locus_tag="RH057_000390"
     CDS             complement(32505..32834)
                     /gene="tusE"
                     /locus_tag="RH057_000390"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HDV3126811.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(32925..33584)
                     /gene="yccA"
                     /locus_tag="RH057_000391"
     CDS             complement(32925..33584)
                     /gene="yccA"
                     /locus_tag="RH057_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HDV3126812.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMVANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(33791..33878)
                     /locus_tag="RH057_000392"
     tRNA            complement(33791..33878)
                     /locus_tag="RH057_000392"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(33842..33844),aa:Ser,seq:tga)
     gene            34305..35423
                     /gene="hyaA"
                     /locus_tag="RH057_000393"
     CDS             34305..35423
                     /gene="hyaA"
                     /locus_tag="RH057_000393"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HDV3126813.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIIAQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            35420..37213
                     /gene="hyaB"
                     /locus_tag="RH057_000394"
     CDS             35420..37213
                     /gene="hyaB"
                     /locus_tag="RH057_000394"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HDV3126814.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLVTASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            37232..37939
                     /gene="hyaC"
                     /locus_tag="RH057_000395"
     CDS             37232..37939
                     /gene="hyaC"
                     /locus_tag="RH057_000395"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HDV3126815.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMVFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWMGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            37936..38523
                     /locus_tag="RH057_000396"
     CDS             37936..38523
                     /locus_tag="RH057_000396"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HDV3126816.1"
                     /translation="MSEQRVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQAALAQLAAWGIVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            38520..38918
                     /gene="hyaE"
                     /locus_tag="RH057_000397"
     CDS             38520..38918
                     /gene="hyaE"
                     /locus_tag="RH057_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HDV3126817.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVLLLSSD
                     PKRTPEVSDNPVMIGELLREFPDYTWQVAIADLEQSEAIGDRFGVFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            38915..39772
                     /locus_tag="RH057_000398"
     CDS             38915..39772
                     /locus_tag="RH057_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HDV3126818.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSESEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            39906..41450
                     /gene="appC"
                     /locus_tag="RH057_000399"
     CDS             39906..41450
                     /gene="appC"
                     /locus_tag="RH057_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HDV3126819.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAITETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            41462..42598
                     /gene="appB"
                     /locus_tag="RH057_000400"
     CDS             41462..42598
                     /gene="appB"
                     /locus_tag="RH057_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV3126820.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSNPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNENELY"
     gene            42611..42703
                     /gene="cbdX"
                     /locus_tag="RH057_000401"
     CDS             42611..42703
                     /gene="cbdX"
                     /locus_tag="RH057_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HDV3126821.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            42783..44081
                     /gene="appA"
                     /locus_tag="RH057_000402"
     CDS             42783..44081
                     /gene="appA"
                     /locus_tag="RH057_000402"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HDV3126822.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESVVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLRAKKGCPQS
                     GQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILSRAGGSIADFTGHRQTAFRELERVLNFPQSNLCLKREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQADGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(44196..46376)
                     /gene="etk"
                     /locus_tag="RH057_000403"
     CDS             complement(44196..46376)
                     /gene="etk"
                     /locus_tag="RH057_000403"
                     /EC_number="2.7.10.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
                     /protein_id="HDV3126823.1"
                     /translation="MTTKNMNTPPGSTQENEIDLLRLVGELWDHRKFIISVTALFTLI
                     AVAYSLLSTPIYQADTLVQVEQKQGNAILSGLSDMIPNSSPESAPEIQLLQSRMILGK
                     TIAELNLRDIVEQKYFPIVGRGWARLTKEKPGELAISWMHIPQLNGQDQQLTLTVGEN
                     GHYTLEGEEFTVNGMVGQRLEKDGVALTIADIKAKPGTQFVLSQRTELEAINALQETF
                     TVSERSKESGMLELTMTGDDPQLITRILNSIANNYLQQNIARQAAQDSQSLEFLQRQL
                     PEVRSELDQAEEKLNVYRQQRDSVDLNLEAKAVLEQIVNVDNQLNELTFREAEISQLY
                     KKDHPTYRALLEKRQTLEQERKRLNKRVSAMPSTQQEVLRLSRDVEAGRAVYLQLLNR
                     QQELSISKSSAIGNVRIIDPAVTQPQPVKPKKALNVVLGFILGLFISVGAVLARAMLR
                     RGVEAPEQLEEHGISVYATIPMSEWLDKRTRLRKKNLFSNQQRHRTKNIPFLAVDNPA
                     DSAVEAVRALRTSLHFAMMETENNILMITGATPDSGKTFVSSTLAAVIAQSDQKVLFI
                     DADLRRGYSHNLFTVSNEHGLSEYLAGKDELNKVIQHFGKGGFDVITRGQVPPNPSEL
                     LMRDRMRQLLEWANDHYDLVIVDTPPMLAVSDAAVVGRSVGTSLLVARFGLNTAKEVS
                     LSMQRLEQAGVNIKGAILNGVIKRASTAYSYGYNYYGYSYSEKE"
     gene            complement(46396..46842)
                     /gene="etp"
                     /locus_tag="RH057_000404"
                     /gene_synonym="yccY"
     CDS             complement(46396..46842)
                     /gene="etp"
                     /locus_tag="RH057_000404"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HDV3126824.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYGMLERASQEWAKRLSR"
     gene            complement(46830..47969)
                     /locus_tag="RH057_000405"
     CDS             complement(46830..47969)
                     /locus_tag="RH057_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HDV3126825.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(48015..50111)
                     /locus_tag="RH057_000406"
     CDS             complement(48015..50111)
                     /locus_tag="RH057_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV3126826.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAYQPAPESEGLQYTTVANQLTALKYNAGFEAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGQSEQLQQQRVEAEDLSAFGRGYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDGGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKRFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLYPMTAEREVPVGQ"
     gene            complement(50111..50857)
                     /locus_tag="RH057_000407"
     CDS             complement(50111..50857)
                     /locus_tag="RH057_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV3126827.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPGEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(50854..51498)
                     /gene="gfcB"
                     /locus_tag="RH057_000408"
     CDS             complement(50854..51498)
                     /gene="gfcB"
                     /locus_tag="RH057_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein GfcB"
                     /protein_id="HDV3126828.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVADQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNSGQDPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGRNV
                     LCNVWQEEVTSTRPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(51605..51910)
                     /locus_tag="RH057_000409"
     CDS             complement(51605..51910)
                     /locus_tag="RH057_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-rich inner membrane protein GfcA"
                     /protein_id="HDV3126829.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEATNGTEATTGTTGTTTTTTG
                     ATTTATTTGGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(52352..52564)
                     /gene="cspH"
                     /locus_tag="RH057_000410"
     CDS             complement(52352..52564)
                     /gene="cspH"
                     /locus_tag="RH057_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HDV3126830.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
     gene            52850..53062
                     /gene="cspG"
                     /locus_tag="RH057_000411"
     CDS             52850..53062
                     /gene="cspG"
                     /locus_tag="RH057_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HDV3126831.1"
                     /translation="MSNKMTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRT
                     LNENQKVEFSIEQGQRGPAAANVVTL"
     gene            53073..53261
                     /locus_tag="RH057_000412"
     CDS             53073..53261
                     /locus_tag="RH057_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HDV3126832.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQICTDHAPLDFTK"
     gene            53236..53466
                     /gene="ymcE"
                     /locus_tag="RH057_000413"
     CDS             53236..53466
                     /gene="ymcE"
                     /locus_tag="RH057_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HDV3126833.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGEM
                     AYNISYLTRVPGTNTLLACCCLSRPEEVNSEY"
     gene            53456..53629
                     /locus_tag="RH057_000414"
     CDS             53456..53629
                     /locus_tag="RH057_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HDV3126834.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFTAREKM
                     TGLESYDVKIKIM"
     gene            complement(53677..54750)
                     /locus_tag="RH057_000415"
     CDS             complement(53677..54750)
                     /locus_tag="RH057_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV3126835.1"
                     /translation="MAENKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQLLLLATNI
                     YIAITFWYWVRYYETASSTTFVARPGGIEGWLPIAGLMNLKYSLVTGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSFFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKNCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPDELYRRLIPQAPMIGH"
     gene            complement(54822..57566)
                     /gene="torS"
                     /locus_tag="RH057_000416"
     CDS             complement(54822..57566)
                     /gene="torS"
                     /locus_tag="RH057_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
                     /protein_id="HDV3126836.1"
                     /translation="MNLTLTRRLWMGFALMALLTLTSTLVGWYNLRFISQVEKDNTQA
                     LIPTMNMARQLSEASAWELFAAQNLTSADNEKMWQAQGQMLTAQSLKINALLQALREQ
                     GFDTTAIEQQEQEISRSLRQQGELVGQRLQLRQQQQQLSQQIVAAADEIARLAQGQAN
                     NAATSAGATQAGIYDLIEQDQRQAAESALDRLIDIDLEYVNQMNELRLSALRVQQMVM
                     NLGLEQIQKNAPTLEKQLNNAVKILQRRQIRIEDPGVRAQVATTLTTVSQYSDLLALF
                     QQDSEISNHLQTLAQNNIAQFAQFSSEVSQLVDTIELRNQHGLAHLEKASARGQYSLL
                     LLGMVSLCALILILWRVVYRSVTRPLAEQTQALQRLLDGDIDSPFPETAGVRELDTIG
                     RLMDAFRSNVHALNRHREQLAAQVKARTAELQELVIEHRQARAEAEKASQAKSAFLAA
                     MSHEIRTPLYGILGTAQLLADNPALNAQRDDLRAITDSGESLLTILNDILDYSAIEAG
                     GKNVSVSDEPFEPRPLLESTLQLMSGRVKGRPIRLATAIADDMPCALMGDPRRIRQVI
                     TNLLSNALRFTDEGYIILRSRTDGEQWLVEVEDSGCGIDPAKLAEIFQPFVQVSGKRG
                     GTGLGLTISSRLAQAMGGELSATSTPEVGSCFCLRLPLRVATAPVPKTVNQAVRLDGL
                     RLLLIEDNPLTQRITVEMLNTSGAQVVAVGNAAQALETLQNSEPFAAALVDFDLPDVD
                     GITLARQLAQQYPSLVLIGFSAHVIDETLRQRTSSLFRGIIPKPVPREVLGQLLAHYL
                     QLQVNNDQSLDVSQLNEDAQLMGTEKIHEWLVLFTQHALPLLDEIDIARASQDSEKIK
                     RAAHQLKSSCSSLGMRIASQLCAQLEQQPLSAPLPHEEITRSVAALEAWLHKKDLNAI
                     "
     gene            57649..58677
                     /gene="torT"
                     /locus_tag="RH057_000417"
     CDS             57649..58677
                     /gene="torT"
                     /locus_tag="RH057_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HDV3126837.1"
                     /translation="MRVLLFLLLSLFMLPAFSADNLLRWHDAQHFTVQASTPLKAKRA
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVASLPVIELVNAIDAPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(58650..59342)
                     /gene="torR"
                     /locus_tag="RH057_000418"
     CDS             complement(58650..59342)
                     /gene="torR"
                     /locus_tag="RH057_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HDV3126838.1"
                     /translation="MPHHIVIVEDEPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDNCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            59472..60644
                     /gene="torC"
                     /locus_tag="RH057_000419"
     CDS             59472..60644
                     /gene="torC"
                     /locus_tag="RH057_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HDV3126839.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHAKQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNQCHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            60644..63190
                     /gene="torA"
                     /locus_tag="RH057_000420"
     CDS             60644..63190
                     /gene="torA"
                     /locus_tag="RH057_000420"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
                     /protein_id="HDV3126840.1"
                     /translation="MNNNDLFQASRRRFLAQLGGLTVAGMLGPSLLTPRRATAAQAAT
                     DAVISKEGILTGSHWGAIRATVKDGRFVAAKPFELDKYPSKMIAGLPDHVHNAARIRY
                     PMVRVDWLRKRHLSDTSQRGDNRFVRVSWDEALDMFYEELERVQKTHGPSALLTASGW
                     QSTGMFHNASGMLAKAIALHGNSVGTGGDYSTGAAQVILPRVVGSMEVYEQQTSWPLV
                     LQNSKTIVLWGSDLLKNQQANWWCPDHDVYEYYAQLKAKVAAGEIEVISIDPVVTSTH
                     EYLGREHVKHIAVNPQTDVPLQLALAYTLYSENLYDKNFLANYCVGFEQFLPYLLGEK
                     DGQPKDAAWAEKLTGIDAETIRGLARQMAANRTQIIAGWCVQRMQHGEQWAWMIVVLA
                     AMLGQIGLPGGGFGFGWHYNGAGTPGRKGVILSGFSGSTSIPPVHDNSDYKGYSSTIP
                     IARFIDAILEPGKVINWNGKSVKLPPLKMCIFAGTNPFHRHQQINRIIEGWRKLETVI
                     AIDNQWTSTCRFADIVLPATTQFERNDLDQYGNHSNRGIIAMKQVVPPQFEARNDFDI
                     FRELCRRFNREEAFTEGLDEMGWLKRIWQEGVQQGKGRGVHLPAFDDFWNNKEYVEFD
                     HPQMFVRHQAFREDPDLEPLGTPSGLIEIYSKTIADMNYDDCQGHPMWFEKIERSHGG
                     PGSQKYPLHLQSVHPDFRLHSQLCESETLRQQYTVAGKEPVFINPQDASARGIRNGDV
                     VRVFNARGQVLAGAVVSDRYAPGVARIHEGAWYDPDKGGEPGALCKYGNPNVLTIDIG
                     TSQLAQATSAHTTLVEIEKYNGTVEQVTAFNGPVEMVAQCEYVPASQVKS"
     gene            63187..63786
                     /gene="torD"
                     /locus_tag="RH057_000421"
     CDS             63187..63786
                     /gene="torD"
                     /locus_tag="RH057_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HDV3126841.1"
                     /translation="MTTLTAQQIACIYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLAAAVNALENRIAALTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQ
                     EIKRLLVEAGMETSGNFNEPADHLAIYLELLSHLHFSLGEGTVPARRIDSLRQKTLTA
                     LWQWLPEFVARCHQHDSFGFYAALSQLLLVLVECDHQNR"
     gene            complement(63879..64184)
                     /gene="cbpM"
                     /locus_tag="RH057_000422"
     CDS             complement(63879..64184)
                     /gene="cbpM"
                     /locus_tag="RH057_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HDV3126842.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVTHP"
     gene            complement(64184..65104)
                     /gene="cbpA"
                     /locus_tag="RH057_000423"
     CDS             complement(64184..65104)
                     /gene="cbpA"
                     /locus_tag="RH057_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HDV3126843.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHSDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPATRGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGHDLEIVVPVS
                     PWEAALGAKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
BASE COUNT        16159 a        16171 c        17183 g        15881 t
ORIGIN      
        1 tcatatttac gaagagatca ggatgatgac gatgaaaaaa agtgtattga cggcgtttat
       61 aactgtggta tgtgcaacgt ccagcgttat ggcagctgat gataatgcta tcacggatgg
      121 caaagtaaca tttaatggta aagttattgc tccagcttgt accctggtag ctgcgacgaa
      181 agattcagta gtcacattgc caaatgtcag cgccaccaag ttgcaaacta atggagcagt
      241 ctctggcgtt aaaactgatg taccaattgc cttagaaggc tgtgatgtta ctgtaactaa
      301 aaatgcgacg tttaccttta gtggtacggc tgacggcgtg caacctacag catttgccaa
      361 ccaggctacc accgatgcag cgacgaatgt tgctctacaa atgtatctgc cagatggttc
      421 tacatcggta actcccggta ccgaaacgtc gaatattcag ttagcggatt ctgctgagca
      481 aacagtgacc tttaaagttg attatatcgc tacggggaaa gcgacttctg gtaatgtgaa
      541 tgcggtaaca aatttccata ttaactatta ttaatagaac tcattaattg ttttattaat
      601 tagtacccct ctagtgttct ggaggggata ttcatatttt ttaagagtga ctatttatga
      661 aaacttgcat aacaaaggga attgtgaccg taagtttaac ggcaatatta ctcagctgct
      721 catccgcatg ggcggcaggt aaaggcggga ttggacttgc agcaacacgt cttgtttatt
      781 cagaaggtga agagcaaatt tcactgggtg tacgtaacac cagtccggat gttccttatc
      841 ttattcagtc atgggtgatg accccagata ataaaaaatc agcagatttt attattacac
      901 caccgctatt tgtgctgaat ccggctaatg agaatctgtt acgcattatg tacattggtg
      961 cgccgttggc gaaagacaga gaaacccttt tcttcactag cgtacgggca gtcccttcaa
     1021 caacgaagcg ggaagaggga aataccctga agattgccac acaaagcgtc atcaaacttt
     1081 tctggcgacc aaaaggttta gcgtatccct taggcgaggc tccggcgaaa ctgcgttgca
     1141 cttcgtcagc tgacatggtt acggtcagta acccaacacc ttatttcatt accctgacag
     1201 acctgaaaat aggtggaaaa gtagttaaaa atcaaatgat ttcccccttt gataaatacc
     1261 aattttctct gccaaagggg gccaaaaata gcagcgtaac gtatcgaacc atcaatgact
     1321 acggggcgga aacgccgcaa ctcaactgta aatcgtaagc cgtcttcagt taagagagcg
     1381 agatgtatag aactcatcga caacacagcc tgttaagctc tggtggagtg ccatcgttta
     1441 ttggtgggct ggtggtgttt gtgtcggcag cgttcaatgc acaagctgaa acctggttcg
     1501 atcctgcttt tttcaaagat gatccctcaa tggtggccga tttgtctcgt ttcgaaaaag
     1561 gacaaaaaat aacgccaggg gtttatcgtg tcgatattgt tctgaatcag acaattgtag
     1621 atacgcgcaa cgtcaatttt gttgagataa cgccagagaa ggggattgcc gcctgtttga
     1681 cgactgaaag cctggatgca atgggtgtta atactgatgc gtttccggct tttaaacaac
     1741 tggacaaaca agcgtgtgtg ccattggcgg agattattcc ggatgccagc gtaacgttta
     1801 atgtgaataa actccgtctg gaaatttcag taccgcaaat cgccatcaaa agtaatgccc
     1861 gtggttatgt cccccctgaa cgttgggatg aagggatcaa cgcgctatta ctgggatatt
     1921 cgtttagcgg ggctaacagt attcatagca gcgcagacag tgattctggc gacagctatt
     1981 ttctgaattt aaacagtggc gttaatttag gcccatggag attgcgcaac aattcaacat
     2041 ggagtcgcag tagtggccaa accgcagaat ggaagaatct cagcagctat ttgcagcggg
     2101 cggttattcc actaaaaggc gaactgaccg ttggtgatga ttatactgca ggcgattttt
     2161 tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga tgacaacatg ctgccagaca
     2221 gcctgaaagg gtttgcgcct gtggtgcgtg gtatcgccaa aagcaatgcc cagataacga
     2281 ttaagcaaaa tggttacacc atttaccaaa cttatgtatc gcctggtgct tttgaaatta
     2341 gtgatctcta ttccacgtcg tcgagcggtg atttgttagt tgaaatcaaa gaagcggacg
     2401 gcagcgtcaa tagctacagc gtaccgtttt ccagcgtgcc attactccag cgtcaggggc
     2461 gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa tagtaatgaa cagcaggaga
     2521 gcaaatttgc ccaggccacg ttgcagtggg gcggaccgtg gggaacgaca tggtatggtg
     2581 gtggacaata tgctgaatat taccgtgccg ccatgtttgg tctgggattt aaccttggcg
     2641 atttcggagc aatttcgttc gatgcgaccc aggcgaagag tacgctggca gaccaaagcg
     2701 aacataaagg tcagtcatat cgttttctgt atgccaaaac gctcaaccaa ttgggcacta
     2761 attttcaatt gatgggctat cgctattcga cgtcgggttt ctacaccctt tccgacacca
     2821 tgtataaaca tatggatggc tacgaattta atgacggtga tgatgaagat acgccgatgt
     2881 ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa actgcaggtc aatatctccc
     2941 agcaattagg cgagtacggt tcgttttatt taagtggtag ccagcaaact tactggcata
     3001 ccgatcaaca ggatcggcta ttacagtttg gctacaacac gcaaattaaa gatctctcgc
     3061 tgggggtttc ctggaactac agtaagtccc gtggtcaacc tgatgctgat caggtgtttg
     3121 cactaaattt ttccctgccg ctcaatctgt tgctccccag aagtaatgat agctatacca
     3181 ggaaaaaaaa ttacgcctgg atgacctcta acaccagtat cgataacgaa gggcacatta
     3241 cacaaaacct gggtttaacg gagacactac tcgatgacgg taacctgagc tacagcgtgc
     3301 aacagggata taacagcgag gggaaaacgg ctaatggtag cgccagcatg gactacaaag
     3361 gggcgtttgc agatgcccga gtgggctaca actacagcga taacggcagt caacaacaac
     3421 tgaactacgc tctttcaggc agtttagttg cccattcaca gggcattacc ctggggcaat
     3481 cgctggggga aactaacgtg ctgattgcag caccaggcgc agagaatact cgtgtggcga
     3541 acagcaccgg gctgaaaact gactggcgcg gatataccgt tgtccctatg ccacttctta
     3601 tcgggaaaat cgaatcgcac ttgatgcggc atcgttaaaa cgtaacgtgg atcttgaaaa
     3661 tgcagtagtc aacgtggttc ccaccaaagg ggcgttggtt ctggcggagt tcaatgccca
     3721 tgcgggagca agggtattaa tgaaaacatc aaagcagggt ataccgctgc gttttggcgc
     3781 gatagcgacg ctggacggca tacagactaa tagcggcatt attgatgatg atggctcgct
     3841 ctatatgtcc ggtttgcccg ctcagggagc gataactgta cgctggggcg aagctcccga
     3901 tcaaatttgt catatcagtt accagcttac cgaacaacaa attaactctg cgattacgcg
     3961 aatggatgcc atatgcagat aatctttgga gaaaaatgcg tgttattact acgactattt
     4021 tttgccgccg tcttaatgct atggtgcgct caaactgctg cttatagcgg gcagtgtcat
     4081 accactcagg ggaatccgta tattggcgtc aattttggcg ttaaaaccct ggaggaagaa
     4141 gaaaatacgg ctggagtagt taaagacaaa ttttatcagt ggaacgaatc gaatgattat
     4201 tatgtttcct gtgattgcga taaagacaat gtcagaagtg gccgatgggc attcgccgcg
     4261 gattcaccgt tagtctattt aggcgacaac tggtacaaaa ttaatgacta tcttgccgcc
     4321 aaagttttat tgcaggttaa aggcagttct cctactgcgg ttcctttcga aaacgtgggc
     4381 acaggggcag atacacgatg gcatatttgc gatcccggcg gtcaacgttt aggtggccag
     4441 ggggctagcg gtaatagcgg tagcttttcc ctgaaaatat tgcagccgtt cgttggttcg
     4501 gtcgtcattc ctcctatggc gctggcgcga ttatttgaat gctacaacat acccgcaggt
     4561 gattcctgca cgactacagg tacatcggtt ttagtgtatt acctgtctgg tacgatcaat
     4621 tcacttggct catgttccgt caatgccgga gagactatcg aggtcgatct gggcgacgta
     4681 tttgcggcta actttcgtgt tgtagggcat aaaccccttg gggccagaac ggcagaactc
     4741 gcaattccag tcaggtgtaa cacgggaaac gcggggttag ttaacgtcaa cctgagtctg
     4801 acggcaacca cagaccccag ctatccccag gcgattaaga cgtcacgtcc tggcgtgggc
     4861 gtggtggtga ccgatagcca gaacaacatt atttcccctg ctggtggaac attaccgctc
     4921 tctattcctg atgatgcaga cagtatcgcg cgaatgaatg tctatccagt cagcacgaca
     4981 ggtgtaccac cagaaaccgg gcgatttgaa gccacggcaa cggtgagaat aaattttgat
     5041 taacgcgtga acgtatgaag aaaaaaacga tatttcagtg cgttatcttg ttctttagca
     5101 ttcttaacat ccatgtcggg atggctgggc ctgaacaagt tagtatgcat atttatggga
     5161 atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc attacaaaat attcatattg
     5221 gtgattttaa tatcagtgat tttcaggccg cgaataccgt aagcactgct gctgatttga
     5281 atatcgatat caccggttgt gccgctggta ttactggcgc ggacgtcctt tttagcggcg
     5341 aggctgacac ccttgcgccg acactgctca aactaactga cacaggcgga agcggtggta
     5401 tggcaacggg gattgccgtg caaattcttg atgcgcaaag tcagcaagaa atcccgctca
     5461 atcaggtcca gcctcttacg cccttaaaag ccggggataa cacactcaaa tatcaacttc
     5521 gttataagtc cacaaaggcg ggagcaacgg gcggtaatgc gacggcggtt ctctattttg
     5581 atctggttta ccagtgaagg agtgatgttg aaacggataa tctggattct gttcttactg
     5641 ggattaacgg ggggctgtga gctatttgcc catgatggca cagtcaacat tagcggatcg
     5701 tttcgccgta atacatgcgt gctggcacag gatagcaagc aaatcaatgt gcagttaggc
     5761 gatgtctcgc tgacacgttt ttttcatggg aattatggcc cggagaaatc tttcatcatt
     5821 aatttgcagg attgcggaac agatgtcagc acggttgacg tcaccttttc aggaaccccc
     5881 gacggagtgc agagcgaaat gctgtccatt gaaagtggta cggatgcagc cagcgggctg
     5941 gcaattgcga ttctggatga tgcgaagata ttgattccgc ttaaccaggc gagtaaagac
     6001 tacagcctac atagcggtaa ggtgccatta actttttatg cgcaattgcg acctgtcaat
     6061 agcgatgtgc agtcgggtaa agtgaatgcc agcgcgacat ttgtacttca ttatgactaa
     6121 tacgtggaac agattggcgc tcttgatttt cgccgtttta tcgctgctgg tggcgggtga
     6181 attgcgggca ggagtggtgg tcggcggaac gcgatttatc tttccggcag acagagaatc
     6241 gatatctatt ttactgacta ataccagtca ggaatcctgg cttattaaca gtaaaatcaa
     6301 ccgcccaacg cgttgggccg ggggggaagc gtcgacagtg ccagcaccat tactggccgc
     6361 tccgccactt attctcctga agcccggtac tacaggcacg ttgcgcttgc tgagaacgga
     6421 aagcgacatc ttgcctgtgg atcgcgaaac gctatttgag ttaagcattg ccagcgtgcc
     6481 atccggcaaa gttgaaaatc agagcgtaaa agtggcgatg cgctcggtat ttaaactgtt
     6541 ctggcgaccc gaaggtttgc cgggcgaccc gctggaagct tatcaacaat tacgctggac
     6601 acggaattcg cagggtgtac aactcactaa cccaacgcct tactacatta acctgattca
     6661 gttgagcgta aatggtaaag cgttaagtaa tgcgggagtt gtgccgccta aaagccagcg
     6721 tcagacaagc tggtgtcagg caatcgcacc gtgtcatgtc gcctggcggg cgataaatga
     6781 ttacggcggg ttgagtgcaa agaaggagca aaatctgccc tgaaacaggt tcggaaaacg
     6841 tttgcgtttt ttttgccgca ggtcaattcc cttttggtcc gaactcgcac ataatacgcc
     6901 cccggtttgc acaccgggaa tccaggagag ttcatgtact accccttcgt tcgtaaagcc
     6961 cttttccagc tcgatccaga gcgcgctcat gagtttactt ttcagcaatt acgccgtatt
     7021 acaggaacgc cgtttgaagc actggtgcgg cagaaagtgc ctgcgaaacc tgttaactgc
     7081 atgggcctga cgtttaaaaa tccgcttggt ctggcagccg gtcttgataa agacggggag
     7141 tgcattgacg cgttaggcgc gatgggattt ggctcgatcg agatcggtac cgtcacgcca
     7201 cgtccacagc caggtaatga caagccgcgt ctctttcgtc tggtagatgc cgaaggtttg
     7261 atcaaccgta tgggctttaa taatcttggc gttgataacc tcgtagagaa cgtaaaaaag
     7321 gcccattatg acggcgtcct gggtattaac atcggcaaaa ataaagatac gccagtggag
     7381 cagggcaaag atgactatct gatttgtatg gaaaaaatct atgcctatgc gggatatatc
     7441 gccatcaata tttcatcgcc gaatacccca ggattacgca cgctgcaata tggtgaagcg
     7501 ctggatgatc tcttaaccgc gattaaaaat aagcaaaatg atttgcaagc gatgcaccat
     7561 aaatatgtgc cgatcgcagt gaagatcgcg ccggatcttt ctgaagaaga attgatccag
     7621 gttgccgata gtttagttcg ccataatatt gatggcgtta ttgcaaccaa taccacactc
     7681 gatcgttctc ttgttcaggg aatgaaaaat tgcgatcaaa ccggtggctt aagtggtcgt
     7741 ccgcttcagt taaaaagcac cgaaattatt cgccgcttgt cactggaatt aaacggtcgc
     7801 ttaccgatca tcggtgttgg cggcatcgac tcggttatcg ctgcgcgtga aaagattgct
     7861 gcgggtgcct cactggtgca aatttattct ggttttattt ttaaaggtcc gccgctgatt
     7921 aaagaaatcg ttacccatat ctaattattt cttcgaattc ctctttataa gataatcagg
     7981 gctttatttt cagccctggt tgttttatat tcatcgctgt tgcttattta gacattttgt
     8041 acttttattg ttgaggttat taagcgaagc gacaatggat tgtgttgctg cggttttata
     8101 gggtagggga gaggcagatg cgaattaaac cagacgataa ctggcgttgg tattacgatg
     8161 aagagcacga tcgaatgatg ctcgatttag ccaatggtat gctatttcgc tcacgttttg
     8221 cgcgcaagat gttgactcca gatgcctttt ctcccgcagg cttttgcgtt gacgacgccg
     8281 cgctctattt ctcttttgaa gaaaagtgcc gcgattttaa tttatctaaa gaacaaaaag
     8341 ccgaactggt gctgaatgca ctggtagcaa ttcgctatct caaaccgcaa atgccgaaaa
     8401 gctggcattt tgtttcccat ggtgaaatgt gggtgcccat gccgggcgat gcggcctgcg
     8461 tttggttgag cgatactcac gagcaggtca atttgctggt cgttgaatct ggcgaaaatg
     8521 ccgcactatg cctgctggca caaccctgcg ttgttattgc gggtcgcgcg atgcagttgg
     8581 gcgacgccat taaaatcatg aacgacaggc tgaaaccgca ggttaatgtt gacagcttca
     8641 gcctcgaaca ggctgtctaa cgcgccaact taagtgcagt cttcggtaca cagctacagc
     8701 aaagaatggt gccatcatcg cccattgctg attttttcag cggcgtgact tcgccttcta
     8761 aaagctgaac acggcaactt ccacaaatgc ccgcgcggca agaataaggg atacgaattc
     8821 cctgattttc taattgctcc agcaacacct gttggttatt tccacgaaat gcctgtccct
     8881 gccagtcaat atctacattt gcgtccggtt gttgcgtgat gttggcggta tcatcagcgg
     8941 cagctgcgcc gtaaattttt gccggagccg ttgccagaat ttccacctca tcgccaacgc
     9001 gaatcacgcc gctattacgg gcaattaaat tctgaccaaa atcgacatcg ccgttatcct
     9061 gggcagtgcg gaaagattgc aatgttttta atggttcgcc tgccggatgt ttttgccctt
     9121 tttctgggct gacggtggtg aaaatacagc ggctacaagg tttaaccaca tcaaacacca
     9181 catcaccaat gcgaatcact ttccagctat cttcttccca ggctgacgcg ccggaaacca
     9241 ccagattggg gcggaattgc tccattttta cactggccgg acaacgttgt tggagatcac
     9301 gtaacgaggc ttcgttagca agaaggtaag gatagccatc agcaaatgac aggggtacag
     9361 tgttgtggcg tttcacgcgc cgggtcattt gtggccccac ccagcgtaat tgcacttcgc
     9421 gggagaaaaa tccacttagc catttgttga tcgcgtctgg cgcaattcgc gcggtaaaat
     9481 gtgtgcccca aacttcggtt ggtgcgtctt gtgtggcgaa atcagcaaaa cgaacatatg
     9541 cgctactgcc atctggtgcg gtgagatgca agccatcatg cacaggtgaa ggagtaaacc
     9601 gtaccatctg gggaaactgg cgagcggtaa taaacgtacc gtcaggttcc gtgatcataa
     9661 agatgcgatc gaaggccaga ccactgacat cagccagagc atgtgtaaga ccaatgccgc
     9721 gcatcgattt aacaggatga ataaaaagcc gggttaatgt cgccacggtg cggtcctcaa
     9781 atgaaaataa gccctcaact ttatgacatg acgcgcttat tagctataat gcgcaacaat
     9841 tttcttagcg cctgtcccat taggctattt tatttgtcag tttggccctg ggttgtgctc
     9901 gaaatcctca cgtacttgtg tacgctccgg tttctccgcg ctgtccatgt ccagactgac
     9961 tgcaacaatt acgcctactg cggtaggctc ttagagtaaa agtgacgata tgaattctct
    10021 gtttgccagt acggcccgtg ggctggaaga gctgttaaaa actgaactgg aaaacctggg
    10081 ggccgttgaa tgccaggtgg ttcagggtgg ggtccatttc aagggcgaca cacggcttgt
    10141 ttaccagagc ctgatgtgga gccgcctggc ctcgcgtatt atgttgccgc tgggcgagtg
    10201 taaggtttac agcgatttag acctctatct cggtgttcag gcgatcaact ggacagagat
    10261 gtttaatcct ggcgcgacct tcgctgtcca cttcagtggt ttgaatgaca ccatccgcaa
    10321 cagtcagtac ggcgcgatga aagtgaaaga cgcgatcgtc gatgctttca cgcggaaaaa
    10381 tctgccgcgt ccaaatgttg atcgcgatgc gccggatatc cgcgttaacg tctggctgca
    10441 taaagaaacc gccagtatcg ctcttgatct gagtggtgat ggtttacatc tgcgtggcta
    10501 tcgcgatcgt gctggtattg cgccgatcaa agaaaccctg gcagccgcta ttgtgatgcg
    10561 atccggctgg cagccaggaa caccgctgct cgatccgatg tgtggttccg gtacgttgct
    10621 gattgaagcg gcgatgctgg cgaccgatcg cgcaccaggc ttgcaccgtg ggcgttgggg
    10681 ctttagcggc tgggcgcagc atgatgaagc tatctggcag gaagtgaaag cggaagcgca
    10741 aactcgtgcc cgtaaaggcc tggctgagta tagctctcat ttttacggtt cggacagcga
    10801 cgcacgggtg attcaacgtg cgcgcactaa cgcccgtctt gcggggattg gtgaactgat
    10861 cacctttgag gtgaaagatg tcgcgcaact gaccaatccg ctgccgaaag ggccgtacgg
    10921 cacagtgttg agcaacccgc catacgggga acgtctggac agcgaaccgg cgctgattgc
    10981 gctgcatagc ctgctgggtc ggatcatgaa aaaccagttt ggtggctgga atctctcttt
    11041 gtttagtgcc tcgccggatc tgctaagctg cttgcagctg cgtgcagaca aacagtacaa
    11101 ggcgaaaaac ggcccgctgg actgcgtaca gaaaaattac cacgttgccg aaagcactcc
    11161 agacagcaaa ccggcgatgg tagcggaaga ctacgccaac cgcctgcgta agaacctcaa
    11221 aaaattcgag aagtgggctc gtcaggaagg aattgaatgt taccgcctgt atgacgccga
    11281 tctgccggaa tataacgttg ccgttgaccg ttatgccgac tgggtggtgg tgcaggagta
    11341 tgcaccgcca aaaactattg atgctcacaa agcgcgtcag cgtctgttcg atattatcgc
    11401 tgcaaccatt tcggtactgg ggattgcgcc aaacaaactg gtgctgaaaa cccgtgaacg
    11461 tcagaagggc aaaaatcagt accagaaact gggcgagaag ggcgagtttc tggaagttac
    11521 cgaatataac gctcacttgt gggtgaatct gacggattac ctcgataccg gtctgttcct
    11581 cgatcaccgt atcgcccgtc gtatgctcgg ccagatgagc aaaggcaaag atttcctcaa
    11641 cctgttctct tataccggca gcgccaccgt gcatgcggga ttaggcggtg cacgcagcac
    11701 caccaccgtg gatatgtcgc gtacttatct ggagtgggca gaacgcaacc tgcgtctgaa
    11761 tggcctaacc gggcgtgcgc atcgcctgat tcaggccgat tgcctggcgt ggctgcgtga
    11821 ggcaaatgaa cagttcgatc tgatctttat cgatccgcca accttctcta actcaaaacg
    11881 aatggaagat gcgtttgatg ttcagcgcga tcatctggcg ctgatgaaag atttgaaacg
    11941 tctgctgcgt gcaggtggga cgatcatgtt ctcgaacaac aaacgtggct tccgtatgga
    12001 tctcgacggg ctggcaaaac tgggactgaa agcacaagaa attacgcaaa aaacgctctc
    12061 ccaggatttc gcccgtaacc accagatcca caactgctgg ctgattaccg cagcctgaaa
    12121 ggaatagtaa tgtcattaat cagtatgcat ggtgcatggc tgtcgttcag cgacgcgccg
    12181 cttctcgata acgcagaact gcatatcgaa gataacgaac gtgtttgtct ggtaggccgt
    12241 aacggcgcgg gcaaatcgac gttaatgaag atcctcaacc gtgaacaggg gctggatgac
    12301 gggcgcatta tttacgagca agatttgatc gtggctcgtc tgcaacagga tccgccgcgt
    12361 aacgttgagg gcagcgttta tgatttcgtt gccgaaggca ttgaagaaca agcggaatac
    12421 ctgaaacgct atcacgatat ttcgcgcctg gtgatgaacg acccgagcga gaaaaatctc
    12481 aacgaactgg cgaaggttca ggaacagctg gatcaccaca acctgtggca gctggaaaac
    12541 cgcatcaacg aagtgctggc gcaactgggg ttagatccta atgttgcgct gtcgtcgctt
    12601 tccggcggct ggttgcgtaa agcggcatta ggacgcgcgc tggtgagcaa tccgcgcgtt
    12661 ttgctgctcg acgaaccgac taaccacctg gatatcgaaa ccatcgactg gctggaaggg
    12721 ttccttaaaa ccttcaatgg gacgattatt ttcatctccc acgaccgttc gtttatccgc
    12781 aatatggcga cgcgcattgt tgatctcgat cgcggcaagc tggtgaccta tccggggaat
    12841 tacgaccagt acctgcagga aaaagaagaa gccctgcgcg tggaagaact acaaaatgcc
    12901 gagttcgatc gcaaactggc gcaggaagag gtgtggatcc gccaggggat caaagcacgc
    12961 cgtacccgta atgaaggccg cgtacgcgcc ctgaaagcaa tgcgtcgcga gcgtggtgaa
    13021 cgtcgcgaag tgatgggtac cgcaaagatg caggtggaag aggccagccg ctccggtaag
    13081 atcgttttcg aaatggaaga cgtttgctac caggttaacg gtaagcaact ggtgaaagat
    13141 ttttctgccc aggttctacg tggcgacaaa attgccctga ttggtccgaa tgggtgcggc
    13201 aaaaccacgc tgctaaaact gatgctcggt cagcttcaag cggacagcgg gcgtattcac
    13261 gttggcacca aactggaagt ggcttatttc gatcagcacc gcgcggaact ggatcccgat
    13321 aaaacggtga tggataacct tgccgaaggt aagcaagagg tgatggttaa cggcaagcca
    13381 cgccacgtat tgggctattt gcaggacttt ctgttccatc cgaaacgggc gatgacgccg
    13441 gtacgtgcgc tttctggcgg tgagcggaac cgcttgctgc tggcgcgttt gttcctcaaa
    13501 ccaagcaact tattgattct tgacgaaccg accaacgatc ttgatgtcga aacgctggaa
    13561 ctgctggaag aactgatcga cagctatcag ggcacggtat tgctggttag ccacgatcgt
    13621 cagtttgtcg ataacaccgt tacagaatgt tggatcttcg aaggcggcgg taaaattggt
    13681 cgttatgtcg gcggttatca tgatgcccgt ggtcagcaag agcagtatgt ggcgctcaaa
    13741 cagcctgcgg tgaaaaaaac cgaagaagcc gccgcggcaa aagcagaaac tgtaaaacgc
    13801 agcagtagca aactaagcta taaattgcag cgcgaactgg agcagctacc gcaattgctc
    13861 gaagatctgg aggcgaagct ggaagcccta cagacgcaag tggcggatgc ttccttcttc
    13921 agtcagccgc atgagcagac gcaaaaagtg cttgctgata tggctgctgc agagcaggag
    13981 ctggagcaag cctttgaacg ctgggagtat cttgaagcgt taaaaaatgg tggctgatcg
    14041 caaaatagtc gatttaaaca gagacagctg acaacggtaa aattgcctga tgaactgggt
    14101 ttatcaggca tgaaaaccaa cactttgtta gtcatctgta aaatagcgca tcattaagga
    14161 gtaccaatgt gcgaacatca tcatgccgcg aagcacatcc tgtgctcgca gtgtgacatg
    14221 ctggtggcgt taccgcgcct tgagcatggt cagaaagcgg catgtccccg gtgtggcaca
    14281 acgttaaccg tggcgtggga tgcccctcgg cagcgtccca ccgcctatgc gttggctgca
    14341 ctgttcatgc tgttgctgtc caacttgttt ccttttgtga atatgaacgt tgcaggagtt
    14401 accagtgaaa ttacattact ggaaattccc ggcgtgcttt tttctgagga ctacgccagc
    14461 ctcggcacct ttttcctgtt gtttgtgcaa ctggttcccg cgttttgtct gataaccatt
    14521 ctgttactgg tgaatcgcgc ggaattaccg gtccgtttaa aagagcaact ggcacgggtg
    14581 ctttttcaac tcaaaacctg gggaatggcg gagattttcc tcgctggtgt gctggtcagt
    14641 ttcgttaaac tgatggctta cggcagcatt ggggtaggca gcagctttct cccctggtgt
    14701 ttattttgtg tcctgcaact gcgcgctttt cagtgcgttg atcgtcgctg gttatgggac
    14761 gacatcgccc cgatgccaga actgcgccag ccgctaaaac caggcgtcac ggggatacgt
    14821 caggggctgc gttcgtgctc ctgttgtacg gcaatccttc ctgctgatga acccgtgtgc
    14881 ccgcgttgta gtaccaaagg gtacgttcgg cgtagaaaca gcctgcagtg gacactcgcg
    14941 ctgcttgtaa cgtccatcat gctgtatctt ccggctaata ttttgcccat catggtgacg
    15001 gatttattag gctcgaagat gccgtcgacg attctcgctg gggtcattct gttatggagc
    15061 gaaggctctt atcccgtcgc tgcggtgatc tttctggcca gtattatggt gccaacgtta
    15121 aagatgatcg ccatcgcgtg gctgtgttgg gatgccaaag ggcatggcaa gcgcgacagt
    15181 gaaagaatgc atttgattta tgaagttgtt gagtttgtag gccgctggtc gatgattgac
    15241 gttttcgtta tcgcggtgct ctcggcgctg gtgcgtatgg gaggtttaat gagtatttat
    15301 ccggcaatgg gtgcattaat gtttgcttta gtcgtcataa tgacaatgtt ttctgctatg
    15361 acgtttgacc cgcgtttgtc gtgggatcgt caacctgaat cagagcatga ggagtcctga
    15421 cagtatggaa tctaataatg gggaagccaa aatccagaaa gtgaagaact ggtctcccgt
    15481 gtggatattt cctatcgtca cggcgctcat tggggcctgg gttctttttt atcattacag
    15541 ccatcaggga ccggaagtga ccctgatcac cgcgaatgcg gaaggaattg aaggtggcaa
    15601 aaccaccatt aaaagccgta gcgttgacgt cggcgtggtt gaaagcgcca cactggctga
    15661 tgatttgacg cacgttgaaa tcaaagcgcg gctgaattcc ggtatggaaa aattgctgca
    15721 taaagacacc gtcttttggg tggtgaaacc gcagattggt cgcgaaggga ttagcggcct
    15781 gggaacgctg ctgtctggag tttatatcga actgcagcca ggcgcgaaag gcagcaaaat
    15841 ggataaatac gatttgctgg actcgccacc gttggccccg cctgatgcga aaggtatccg
    15901 tgtgattctc gatagcaaaa aagccgggca gctctcgcca ggagatccgg tgctgttccg
    15961 tggctatcgg gtaggttcgg ttgaaaccag caccttcgat acacaaaaac gcaatatcag
    16021 ctatcaactg ttcatcaatg caccttatga ccgactggtg accaacaatg ttcgcttctg
    16081 gaaagatagt ggcattgcgg ttgatctgac gtcagcaggg atgcgtgtgg agatgggctc
    16141 attgacaacg ctgctgagtg gcggtgtcag ctttgatgtg ccggaaggtc tggatttagg
    16201 gcagccagtg gcaccgaaaa cagctttcgt tttgtatgat gatcagaaga gcattcagga
    16261 ttcgttgtac accgatcaca ttgattatct gatgttcttt aaagattcgg tacgcggtct
    16321 gcaaccggga gctccggtag agttccgggg tattcgcctg ggtaccgtaa gcaaagtgcc
    16381 attctttgcg ccgaatatgc gtcagacatt taacgatgat taccgtattc cggtactgat
    16441 tcgtatcgag ccagagcggc tgaaaatgca gcttggcgaa aatgcggatg ttgttgagca
    16501 ccttggcgaa ttgttgaaac gtggtttacg cggatcgctg aaaaccggaa acctggtcac
    16561 tggtgcactg tatgttgatc tcgatttcta tccaaatacg cctgcaataa ccggtattcg
    16621 tgaatttaat ggttatcaga ttatcccgac cgttagcggc ggcctggcgc aaatccagca
    16681 acgactgatg gaagcgttgg ataagatcaa caaactgcca ttgaatccga tgattgaaca
    16741 ggcaaccagt acgctttctg aaagtcagcg cacaatgaaa aacctgcaaa cgacgctgga
    16801 tagcatgaac aagatcctcg ctagccagtc gatgcagcag ttgccgacgg atatgcagtc
    16861 aacgttgcgt gaattgaatc gcagcatgca gggcttccag cctggctccg cagcctacaa
    16921 caagatggtg gcggatatgc agcgccttga tcaggtgttg cgagaactgc aaccggtgct
    16981 gaaaacgctc aatgagaaga gtaacgcgct ggtatttgaa gcgaaggaca aaaaagatcc
    17041 agagccgaag agggcgaaac aatgaaaaag tggctagtga cgattgcagc actgtggctg
    17101 gtcggatgca gctccggcga aattaataaa aactattacc agttacctgt ggtgcagagc
    17161 ggtacacaaa gtaccgccag ccagggcaat cgtctgttat gggtagagca ggtcactgtt
    17221 cctgactatc tggcggggaa tggtgtggtt tatcaaacca gtgatgtgaa gtatgtgatt
    17281 gccaacaaca acttgtgggc cagcccgttg gatcaacagt tgcgcaacac cctggttgcc
    17341 aacctgagta cgcaactgcc cggctgggtg gttgcctccc agcctctggg aagcgcccag
    17401 gacacgctca atgttaccgt aacggagttt aacggtcgct atgatggcaa ggtcattgtc
    17461 agtggtgagt ggctgttgaa ccaccaggga caactgatca aacgtccgtt ccgtctggaa
    17521 ggagtgcaaa ctcaggatgg ttacgatgag atggttaaag tgctggccgg tgtctggagt
    17581 caggaagccg cttctattgc acaagagata aagcgtctac cttaattata aagatttgta
    17641 aatataaccg tctccggtat gttgcctgag gcggtttttt tgtctctaac gtgcggaaaa
    17701 atttgttcct cttcacattt tttgtacaac cgacatgccc gtgtagctca caaatatgac
    17761 agtggcgtga attttgcgca ttgacggcag ttatgattcg cggtattgct taactgtgat
    17821 tgcacattta gtaatcactg ttttcttttc caccagaaac cagtatgagg gaaacgaggc
    17881 atgaagagac aaaaacgaga tcgcctggaa cgggcacatc aacgtggtta tcaggccggc
    17941 atcgccggac gctcaaaaga aatgtgtccc tatcagacgc tgaatcaaag gtcacaatgg
    18001 ctgggaggct ggcgagaagc catggcggac agggtagtaa tggcctgatt ctgtctcttt
    18061 aaaaagaaac ctccgcattg cggaggtttc gccttttgat actctgtctg attataatca
    18121 gaaggcagac gtatcctgga acagaccgac tttcaggtcg ctggcggtat agatcagacg
    18181 accatcaacc agcacttcgc catccgccag gcccataatc agacgacggt taacaatgcg
    18241 tttaaagtga atacggtagg tcactttttt cgctgtcggc agtacctgac cagtgaattt
    18301 cacttcgcca acgcccaacg cgcggccttt accttcgccg cccagccagc cgaggtagaa
    18361 ccctaccagc tgccacattg cgtccaggcc caggcatccc ggcataaccg gatcgccaat
    18421 aaagtggcat ccgaagaacc acagatccgg attgatatcc agttctgctt caacataccc
    18481 tttgtcgaag tttccacccg tttcggtcat tttgaccaca cggtccatca tcagcatgtt
    18541 cggtgctggc aattgcgggc ctttagcgcc aaacagttca ccgcgaccag aggcaagaag
    18601 gtcttctttt gtataggatt cgcgtttatc taccatgttc tctgtaagcc ttattttatt
    18661 gaagcacgca ggatagctaa cacgtgtacg ctgaacaagt ccgatcagtt cggaataaac
    18721 cagttcagcc aacgtaatgg ccatggaaaa cggtgacgtc cttcctgttg cgatgcttgc
    18781 gcgatacgtt cctggatggt ttgcatcagc gtcgtttggc cttcgccatc ccacaccaga
    18841 tttaataata acggcagtgc gtcagtcaca tcgtctactg cccagatggt gaatttgcct
    18901 tcttctaccg ctttcaccag ttcactgtga agacttaaat ggcgaacgtt agctgtgggg
    18961 ataatgacac cttgtttccc ggttaactca cgttgctggc aaatagcaaa gaagccttcg
    19021 attttctcat ttaaaccacc gaccggctgg gcgcgaccga actgatcgac tgaacctgtg
    19081 atagcgatac tctgattcac cggcacatcg gcgagggcgc ttatcagggc gcagagttca
    19141 gccatcgagg cactatctcc atcaacttca ctgtatgact gctcaaatgt cagcgatgct
    19201 gagaagggga tctgttgctc aagctgtagt tccgacatca ggaacgcttg catgatcatc
    19261 atccctttcg catggatatt gccgccaagc tccgctttgc gttcgatgtc ggtgaattca
    19321 ccatcgccaa tatgcacaac gcagctaatg cgagaaggtt cgccaaaagc gcgtggatga
    19381 cccggaaatt caatgaccga aagggcgtta atttgcccga tgcgttcgcc ttcggtttca
    19441 atcaggattt gctcctgaag gatctcatcc tgcatacgtt cagcgaggaa accttcgcgc
    19501 cattcacgct gctgcagcat taagtttagc tgctcgccgg agaaggtgtc gccatcacac
    19561 agggaggcga cctctttaca ctggcggagg atccactgcg ggctaagcgg aagtgtttct
    19621 tgttcaccgg tgtagcgtgc tgcttcgcgg ataagtaccg gccaggcatc cgctcccggt
    19681 gcaggtaagt gattatgtct ggcggtaaat gtcacccagc gacaccactg ggttactgac
    19741 tccgcatcga caatctgcag agtatcttca aattcgctat aaatagcctg ctctgaaagc
    19801 tctggctcca tctcctggaa atcagccaat gattcgcgtt cgcctaccag aatgactttc
    19861 agcttcaatg gcatcgaagg cacagagacg gggagagggc gcgactcatc aaacgcaacc
    19921 cagtcaaaac gctcgcggtt aacgatattt ttcagccgca tccacagcag aggttgcgcc
    19981 agcagtgtac gcaaagagat gatgagaata ccgccatttg cctgatgcac cagaccaggc
    20041 tgcagggtaa tgtcgccatt aaactggcgc aggcagccaa agagttgctc cgcttctacc
    20101 cagtcggcag cgacaacttg cgttaaagtc gcaaaattat catctgcact cactgcgtga
    20161 cgtaagcgga tggagtggcc ggaaacctca tagtgaccgc ccaccagttg gcctgcatcg
    20221 ctttgtagcg tacgcgcggc attggcaata agattcagat actcagactc ttccggggcc
    20281 ttcgccagca taaaagagga ggatgctcgc gtatgcagca actgctccag tgcgaattgc
    20341 agtcgcggtt gagtatcact gaataaagga tcgttttcgt caatcaaatg tggctgagca
    20401 aatatttcct gatagctatc ggtatcagga accaggtcac gccatgcaag tttcgtaatg
    20461 gtcaaagttg atgtttttta gtctgttgtc aaagccgcga ttataccgta accggcacta
    20521 cagcacacgt agaaagcacc gacaatactc ctggcatggg cgttaaagct cacaggatgg
    20581 agattctttt cttcactggc ctaaaaagct gatattctgt aaagagttac acggtaacat
    20641 tgagatcgct atgaaatatc aacaacttga aaatcttgaa agcggttgga aatggaagta
    20701 tctggtgaag aagcatcgcg aaggggagtt aatcacccgt tacatagaag ccagtgccgc
    20761 ccaggaagcc gtcgatgtgt tgctctcgct ggaaaatgaa cccgtgctgg taaatggctg
    20821 gattgacaag catatgaatc cggaactggt caatcgcatg aagcaaacca ttcgggcaag
    20881 acgaaagcgc cattttaatg cggaacacca gcatacgcgc aaaaaatcga tcgatctgga
    20941 atttattgtc tggcaacgtc tggctggtct tgcgcagcgt cgcggtaaaa cgctttctga
    21001 aacgattgtt cagctgattg aagatgcgga aaacaaagag aaatacgcga ataaaatgtc
    21061 ttctctgaag caggatctgc aggcattgct gggtaaggaa taactgacga aagtcagttc
    21121 aatttactaa aggcaaaaaa aaccccgcag cagcggggtt tttctaccag acgagaactt
    21181 aagcttgcgg ctgagttaca acgtctttga tacctttaac ttcgatctct acgcgacgat
    21241 ccggagccag gcagtcgatc agtgcagcac gctgtttcac gttgtcacag gtgttgccag
    21301 taaccgggtt ggattcgccc ataccacgtg cggagatctt gtctgccggg atacctttgg
    21361 agatcaggta atcaacaaca gactgagcac ggcgctcgga cagaccctgg ttgtaagcgt
    21421 cagaaccgat gcggtcggtg taacccagaa caactacgga accgtctttc ggatccaggt
    21481 tgctcagctg gctgtacagc tgatccagag cagcctgacc ttccggtttc agggttgctt
    21541 tgttgaagtt gaacagaacg tcagacttca gagtgaagtg cttggtctgt acttccggtg
    21601 ccggagctgg agccggagca actactggag ctgcttcgcc ctgaccgaaa cggtaggaaa
    21661 cacccaggct cagcatgccg ttgtccggac gagtgccgat ggtgtgtgcg tcaccgatgt
    21721 tgttggtcca ctggtattcc agacgggtag cgatttcagg agtgatcgcg tactcaacac
    21781 cgccagcgaa gaccggagaa acgccggtgt cgtggttttt accataaacg ttggatttag
    21841 tgtctgcacg ccataccata ccacccagac gagtgtagat gtccaggtcg tcagtgattg
    21901 ggtaacccag tttagcggtc agttgaacgc cctgagcttt gtatgcaccg ttttcaacgc
    21961 tgcctttgta cggcatacga cctaaccagt cgtaacccat ttcaaagcca acatacgggt
    22021 taacctggta accaccaaaa gcaccagcgc ccagttggtt ttcatgggtc gggccattgt
    22081 tgttgatgaa accagtgtca tggtactggg accagcccag tttagcacca gtgtaccagg
    22141 tgttatcttt cggagcggcc tgcgctacgg tagcgaaacc agccagtgcc actgcaatcg
    22201 cgatagctgt ctttttcatt ttttgcgcct cgttatcatc caaaatacgc catgaatatc
    22261 tccaacgaga taacacggtt aaatccttca ccgggggatc tgctcaatat taactctacc
    22321 gatatcttcg gcttatgccg agcacccctg gcgatgtaaa gtctacaacg tagttgaaaa
    22381 cttacaagtg tgaactccgt caggcatatg aaaaaaaagt cttgtataag gtatgtttaa
    22441 tcttttttgt cagcgacaat ttacagaaga aaatcgcgga aaccgcttca gacaagcctc
    22501 cgcaaggaaa attagtcacg actgaaagca ttggctgggc gacaaaaaaa gttccaggat
    22561 taatcctaaa tttacttaat gatacaaatt agagtgaatt tttagcccgg aaagttgtct
    22621 cgtggcgtga gaggatgcgc ttaccggacg cataataaac cccatagcgt taccttcatt
    22681 tgccgcatca acaagttcag catgctcttc ttcagtcaaa tcatctgcca accaaccgat
    22741 caccacactg taattgcccg tgcgtaaagc gcgaaccatt gactccacag tgtggcaagg
    22801 ggagagctgg ctaatctgca ttactttcgt taagggtagc ccagatgcct gaacccattc
    22861 ccgactcagt ttttgttgcg gtgttaacca gagttgccag cgcgattgct gaccgagttg
    22921 ctgtaacaat ggcaacagta gaagttgcgt catcatgggc tgatcttcgc gatagacaac
    22981 ttcactgata agcccggctg tagtgttttc cgtagagaca cgcgcaattt tacttgctgc
    23041 ggatgagaac gacgaagaac gatgtgcata gcctgaagtg tacataatca atccagcccc
    23101 tgtgagttac tgtatggatg tacagtacat ccagtgacaa caaagatcaa ccctattttc
    23161 ggaaagagcc tcgcaaattt tgtcgttggt gacgggaaaa cataaattaa tcttgcccct
    23221 taagaataag ttgcctattt tcgtagttaa cggatccgtt aatgtgaatc attcttttat
    23281 gttatgattt taaaaggaat tttatgaaaa gcctctccta taagcggatc tataaatcac
    23341 aagaatacct ggcaacgttg ggcacaattg aataccgatc attgtttggc agttacagcc
    23401 tgaccgttga cgacacggtg tttgcgatgg tttctgatgg tgagttgtat cttcgggctt
    23461 gtgagcaaag tgcacagtac tgtgtaaaac atccgcctgt ctggctgaca tataaaaagt
    23521 gtggccgatc cgttaccctc aattactatc gggttgatga aagtctatgg cgaaatcaac
    23581 tgaagctggt gcgtctgtcg aaatattctc tcgatgcagc gctgaaagag aaaagcacgc
    23641 gcaatacccg ggaaagactg aaagatttgc ccaatatgtc ttttcatctg gaagcgattc
    23701 tcggggaggt ggggattaag gatgtacggg cgttacgtat acttggggca aaaatgtgtt
    23761 ggttgcgact gcggcagcaa aacagtctgg tgacagaaaa gattctgttt atgcttgaag
    23821 gtgccattat cggcattcat gaagctgcgc tcccggtggc acgccgccag gagcttgcag
    23881 aatgggctga ctctcttacg ccgaaacagg agtttcctgc ggaacttgag taatctggcg
    23941 ttgcagacga ccaatctcag gcagtaatgc aatcaataat ccaacttgtt gtacgaccag
    24001 aggttctttg ctgtctgccc gtggttcaag ttgctgcatc cgctgtttca ggctagctaa
    24061 tgcctcattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat caacatagca
    24121 aactgcctca tcaagaaacg ccagaatttc aggattagtt aactgctccc ggtgagcacc
    24181 gagggctgag atatagctgg taaacgtatg gttaaggcac agcaaccgaa acgcggcttc
    24241 gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg acgccagttc
    24301 agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt tatcacgccc
    24361 ctgatggtat tgctccagta tggcatcgag ataccggcag ttggcctctg tggcgcgttc
    24421 gagcattcgc ggcagattgc gaaactgcca gtcaggccag atgtagctca ctgccgccca
    24481 cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt caaaaccttc
    24541 acccagcaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg catgggcata
    24601 ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagtagca ccagctgccc
    24661 ttccagtgac ggtacaaacc acagcacagg aatgccaatg gcgataccga ccagcgtacc
    24721 aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc agacaaacaa
    24781 acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga tggcgtagcc
    24841 gaagcacaac accagcgaca ttcttatcgc atgacggaag agggcggatt ccggcgtgaa
    24901 gtgacggcta agacgcagcc agatatcact caacccgtgc gggctgtcat cagcgagctc
    24961 attttcgtca ttattatggg gtagtgcctg ggcctgttct gattcaattg ttgccagttg
    25021 ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta aatcggcggg
    25081 tgcgccgtta tcgcgcatcc gctccagcgc agcatcaata tgcgtaaaag cgcgctcaaa
    25141 atgcggatca tgttgataag gctgacgcaa caaaatacag cgtgacagtt gctggcacgc
    25201 ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt ggcgaaaatg
    25261 ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa tatcctgtgc
    25321 gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc cacgtaagcg
    25381 ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agcagaccgt tggcgagagc
    25441 caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca tgcgcgactt
    25501 gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct gcagcgggcg
    25561 gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg caccggccag
    25621 cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca tagtgtaaat
    25681 ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac cgccgagcaa
    25741 aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc agggaaacag
    25801 caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga ggttacgcaa
    25861 acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc ccagcgttag
    25921 cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc cacaaagcgc
    25981 aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt tgagcaaagg
    26041 acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat tggcttcacg
    26101 cgcagcttgc gccgtttcta ccgataccac gcgacgacca accggccata gtgcaatagc
    26161 ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac attgtgcgat
    26221 gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc agaaaatatt
    26281 caataccgta ccgccagtat tcagcagcac atttttgcca gccgggttca gttcatcgac
    26341 atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc agcaggatcg
    26401 tgtcagcggt aaggtaaaaa tcagcataat actgaccaga gtcgccaaca gccagcccag
    26461 agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac gcataaaacc
    26521 tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg ggctggagca
    26581 cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac agagtcatgg
    26641 aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat cgggcggtga
    26701 tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt cctttcaatc
    26761 aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa tttgtactgc
    26821 cggacgaaaa agtggtgcgt ctgcacggca ccgaatgggg cgagacgcag cgtttttacc
    26881 atcatcttga tgctcactgg cagcggtgga gtggcgagat gagcgaaatt gcgtctggtg
    26941 ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa tggctgacgc
    27001 gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tatgtcggcg ttgccgttgc
    27061 cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa tgtcaggcct
    27121 ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc gaagccatgc
    27181 ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat ccggcgcagg
    27241 cccgggcagt cgttaatggc gagcattctc tgttagtgct ggcaggtgca ggaagcggaa
    27301 aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa gcgtcccctg
    27361 agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac gagcggattc
    27421 gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgctg gcgctgcata
    27481 ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat gataccgctg
    27541 cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag aaagcgcagg
    27601 cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca gaaggtaact
    27661 tctgggatga tgaaaaatta cagcgtcgtc ttgcctcacg cctcgatcgt tgggtaagtc
    27721 tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc gaagagattc
    27781 gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc tggaaaggtg
    27841 cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg attgtgattc
    27901 tggagaaagg tcgctttatc agcccgtgga agcatattct ggttgatgaa tttcaggata
    27961 tctcgccgca gcgggcagcg ttgttagcgg cattacgcaa gcaaaacagt cagacgacgt
    28021 tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg caaatgtcgc
    28081 tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta gacacgactt
    28141 accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag aacccaggcc
    28201 agttgaaaaa gccgctaaac agcttaacca atggagacga aaaagccgtc acgttattgg
    28261 atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa ccggaagagc
    28321 gcattcttat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa aaagcggcaa
    28381 cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa gggcaacagg
    28441 cggattacgt catcatcgtt ggcttgcagg agggaagtga tggttttccg gctgcggcgc
    28501 gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg gacgctgaag
    28561 aacggcggtt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg gcactgttta
    28621 acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg ccggtagcga
    28681 gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca gacggtccgc
    28741 gagataacgc tgataatcgg ggatcagaat atcgaccgcg tcgttgaaat gcggcgactg
    28801 gattatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata ccgtcgccag
    28861 acgcaacaag gctttcacgt caggatcgtg cggcacggca tttagtgggt cccagaagaa
    28921 aatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt caccccccat
    28981 tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatta agttaccggt
    29041 agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt ccacccagct
    29101 catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc gcgcaggtaa
    29161 agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca gattactgta
    29221 agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca aaaatctcag
    29281 gaagatggtt gtttttccgc ccactgtagg aaagtatttc gcgtttgtgg gtcagccagt
    29341 ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg aatagcatcc
    29401 agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc aaattgcggt
    29461 agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata atcaatcgtt
    29521 tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt cgcatcgcca
    29581 tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc attctccagg
    29641 cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac caccagcggt
    29701 ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc aacgcgaaac
    29761 actaactgat gcggtccatt atccagttca atgctgtccg caccgcgcag cagagagctg
    29821 gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg cagcgtggtg
    29881 gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc ggttttcata
    29941 ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc tttaatcata
    30001 cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat tagctgcatg
    30061 gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat aatgaaagaa
    30121 accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg ggcaagcgac
    30181 aaacccgatc gtcccagcta tcgcgtgatg aaatatctac tcgaccaggg gtatcacgtc
    30241 attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg gtatggcacg
    30301 ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc agaagcggcg
    30361 tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat gcaactgggg
    30421 gtgattaatg agcaagcggc agtactggca cgggatgccg ggttgaatgt ggtgatggat
    30481 cgctgcccgg ctattgagat ccctcgcctg ggcctggcca aataaaaaat ccccggaagg
    30541 caaaaacctt ccggggattc gttcagggat cagttacgca gacgcggggc ctggagttgt
    30601 ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc atcctgcaat
    30661 tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt atcgtcctcc
    30721 atcactacgt gataccacgg agcggcgcga agctcgtcat ttaccgccag ctcatcaggc
    30781 gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag ataacctaac
    30841 agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt cacctcccgg
    30901 gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat gacgcgacag
    30961 gcaaaccctt tcagatatag cccttccgga taggtagcga tcaccggatg atcggctgcc
    31021 tgacggaact gctctataaa ttgtacatca cggccggcat caattgcggc atccgcgatg
    31081 attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag gagaataccg
    31141 ccttcattca gcaactgaat cgccagcatg ttgatatctt tatagccacg acacgcgccc
    31201 atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac gtcaaatttt
    31261 tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac aaactcagcc
    31321 ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag cgcttcctgg
    31381 gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac ggcgaaacca
    31441 ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg ggtagccagg
    31501 cggctatcac gctggtccag gtagtagccc gttttgtgtc cgtgctgaat atccaccagc
    31561 agtttcattc cgtgttcttc aatcggcagc agggcaggtg gcaactcgcc ggtgacgggg
    31621 ccctgggtca gctccattcc ttctttttta cgtaccgcaa catcgctgcg atcgtaaatc
    31681 gaacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg atattctgcc
    31741 ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt aatacccggc
    31801 aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt ttgcgccagc
    31861 cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat gtcgatagac
    31921 tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga ataagcgccg
    31981 cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag gctggcttta
    32041 ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag taatgatttt
    32101 tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt cgatgcaaag
    32161 aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt aaggataagg
    32221 agagcagcaa tgtcgaaagt ctgcataatt gcctgggttt acgggcgagt tcagggcgta
    32281 ggatttcgct acaccacaca gtacgaagcg aaaagactgg gtttaaccgg gtacgccaaa
    32341 aatcttgacg atggcagcgt ggaagtggtt gcttgcggtg aagaagggca ggtggaaaaa
    32401 ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtggaacg ggtgcttagc
    32461 gagccgcatc atccctcggg ggaattagcc gattttcgta tccattaaat acattttacc
    32521 ggttttggca ggccagcaat tttggtggct tgcttagccg gacctttcgg aaacagtcga
    32581 tataaatagc ggctgttacc tttttcttcg ccaaatttat tcgccatcgc ttttaccagc
    32641 atacgaatcg ccggagaagt attgaattcc agatagaaat cacgcacaaa acgcaccact
    32701 tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac agccagcggc
    32761 tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat ctctttacct
    32821 tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta acaaaaaaca
    32881 aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc gcggctggcg
    32941 aagcccagaa tgctcagcag gctgacgaag atgttgtaca gcgaaacata caggctaacc
    33001 gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc aaacaaaata
    33061 gcgccagagg agatcaaaat gaagaccgcg ctgatcgcca gatgcagagc aggcagttgc
    33121 aggaagatat tagcaaccat accaatcagc accaccacaa tacccgccat cagcataccg
    33181 ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca gaagaacact
    33241 aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga cagataggtg
    33301 ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc ggagataatc
    33361 ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat acccaccagc
    33421 gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc ggtaatcgcc
    33481 gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt atgggtgcta
    33541 agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc actctctatg
    33601 acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag aagttttacc
    33661 catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc attaagcaaa
    33721 tataacgccc tgagaatttc gacaggcaaa agaaaaaggg gttagcattt agctaacccc
    33781 ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg gtcgccggtt
    33841 ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc actataaaca
    33901 tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag ccaaaagtgc
    33961 attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc cttctctttt
    34021 actcgtttag caaccggcta aacatcccca ccgcccggcc aaaagaaaaa taggtccatt
    34081 tttatcgcta aaagataaat ccacacagtt tgtattgttt tgtgcaaaag tttcactacg
    34141 ctttattaac aatactttct ggcgacgtgt gccagtgcag aaggatgagc tttcgttttc
    34201 agcatctcac gtgaagcgat ggtttgcctt gctacaggga cgtcgcttgc cgaccatgtg
    34261 cgcccggtgt cctgccggtg tcgcaaggag aagagacgtg cgatatgaat aacgaggaaa
    34321 cattttacca ggccatgcgg cgtcagggcg ttacccggcg cagctttctc aaatattgta
    34381 gtctggctgc cacgtcgctg ggattaggcg cgggaatggc accaaagatt gcctgggcgc
    34441 tggagaacaa accccgcatt ccggtggtat ggatccacgg tctggaatgc acctgctgta
    34501 ccgaatcttt tatccgctcc gctcacccac tggcgaagga cgtcatcctt tccctgattt
    34561 ccctcgatta cgacgatact ttgatggctg ccgccggaac ccaggcggaa gaagtctttg
    34621 aagacatcat cgcgcaatac aatggcaaat atatcctcgc agtagaaggt aatccgccgc
    34681 tgggcgagca ggggatgttc tgtatcagca gcggtcgacc gtttattgag aaactcaaac
    34741 gtgccgctgc cggagccagc gcgattatcg cctggggaac ctgcgcgtcc tggggctgcg
    34801 tgcaggccgc gcggcccaat ccgacgcagg caacgcctat cgacaaagtc atcaccgaca
    34861 aacccattat caaagtacct ggctgcccgc cgatcccgga tgtgatgagc gccatcatta
    34921 cttacatggt gacctttgat cgcttgccag atgtcgacag aatgggccgt ccattgatgt
    34981 tctatggtca gcgaatccac gataaatgct atcgccgcgc ccacttcgac gccggagagt
    35041 tcgtccagag ttgggatgat gacgctgccc gcaaaggtta ctgcctgtac aaaatgggtt
    35101 gcaaagggcc aaccacctat aacgcctgtt cctccacccg ctggaatgat ggcgtttctt
    35161 tcccaatcca gtctggtcac ggctgcctgg gctgtgcgga aaatggtttc tgggatcgcg
    35221 gttcgttcta cagccgcgtg gtcgatattc cgcaaatggg tactcattcc actgccgata
    35281 ccgtcggttt aaccgcgctt ggcgtggtgg cagcggctgt tggtgtgcac gcagtcgcca
    35341 gcgccgttga ccagcgcaga cgtcataacc agcaacctac agaaaccgaa catcagccag
    35401 gcaatgagga taaacaggca tgagcactca gtacgaaact cagggataca ctatcaataa
    35461 tgccggacgc cgcctggtgg tcgacccgat tacgcgcatc gaaggccaca tgcgctgcga
    35521 agtgaatatt aacgatcaga atgtgatcac caatgccgtc tcctgcggca ccatgtttcg
    35581 cgggctggag atcattctgc aagggcgcga cccgcgcgat gcgtgggcgt tcgttgaacg
    35641 tatctgcggc gtctgtactg gcgtacacgc cctggcttcg gtttacgcca tcgaagatgc
    35701 tatcggtatt aaagtgccgg acaacgccaa tatcatccgc aacattatgc tggcaacgct
    35761 ctggtgccac gatcatctgg tgcacttcta tcagcttgcc gggatggact ggatcgatgt
    35821 gttagatgcg ctgaaagccg acccgcggaa aacctccgaa ctggcgcaaa gtctctcctc
    35881 ttggccgaaa tcatccccgg gctatttctt cgacgtacaa aaccgcctga aaaaattcgt
    35941 tgaaggcggg cagttgggga tcttccgcaa tggctactgg gggcacccgc agtacaagtt
    36001 gccgccagaa gccaacctga tgggctttgc ccactacctc gaagctctcg atttccagcg
    36061 tgaaattgtg aaaatccacg cggtctttgg cggtaaaaac ccgcatccaa actggattgt
    36121 cggcgggatg ccttgcgcca tcaacattga cgaaagcggc gcggtcgggg cagtcaatat
    36181 ggaacgcctg aacctggtgc agtcaattat cacccgcacg gcggacttca ttaacaacgt
    36241 gatgatcccc gacgccttag ccatcggtca gttcaacaaa ccgtggagcg aaatcggcac
    36301 tggtctttct gataaatgcg ttctcagcta cggcgcattc ccggatattg ccaacgactt
    36361 tggcgagaaa agtctgctga tgcctggcgg cgcggtgatt aacggcgact tcaacaatgt
    36421 gctgccagtg gatttggttg atccgcagca ggtgcaggag tttgtcgacc acgcctggta
    36481 tcgatatccc aacgatcagg tcgggcgtca tccgttcgat ggcatcaccg acccgtggta
    36541 caaccccggc gatgtcaaag gcagcgatac caacattcag cagctgaatg aacaggaacg
    36601 ctactcgtgg atcaaagcgc cacgctggcg cggtaacgcg atggaagtgg ggccgctggc
    36661 gcgcacgtta atcgcttatc acaaaggcga tgctgcgacc gttgagtcgg tcgatcgcat
    36721 gatgtcggcg ttgaacctgc cgctttccgg tatccagtca acgttaggcc gcattttgtg
    36781 ccgcgcgcac gaagcgcagt gggccgcagg taagttgcag tatttcttcg acaagctgat
    36841 gaccaacctg aaaaacggca atctcgtcac tgcttccacg gaaaaatggg aacctgcaac
    36901 ctggccgaca gagtgccgtg gtgtcggttt taccgaagcg ccgcgcgggg cgttaggcca
    36961 ctgggccgcc attcgcgatg gcaagattga tctctaccag tgcgtggtgc cgaccacctg
    37021 gaacgccagc ccgcgcgatc ccaaagggca gattggcgct tatgaagcgg cgctgatgaa
    37081 caccaaaatg gcgatccccg agcaaccgct ggagatcctg cgtactctgc acagctttga
    37141 cccgtgcctc gcctgttcaa cacacgtgct gggcgacgac ggtagcgagc tgatctccgt
    37201 gcaggtgcgt taacagcgaa ggagaatcat catgcaacag aaaagcgaca acgttgtcag
    37261 ccactatgtc tttgaagcgc cagtccgcat ctggcactgg ttgacggtgt tatgcatggc
    37321 ggtgttgatg gtcaccggat actttatcgg caagccgcta ccttccgtca gcggcgaggc
    37381 gacgtatctg ttctatatgg gctacatcag gttaattcac ttcagcgccg ggatggtttt
    37441 taccgtggtt ttgctgatgc ggatctactg ggcttttgtt ggcaatcgat actcccgcga
    37501 gctgtttatc gtgccggtat ggcgtaaaag ctggtggcag ggcgtgtggt atgaaatccg
    37561 ctggtatctg tttctggcaa aacgtccgag tgccgatata ggccataatc ccatcgccca
    37621 ggcggcgatg ttcggctatt tcctgatgtc ggtctttatg atcatcactg gttttgcgct
    37681 gtatagcgaa cacagccagt acgctatttt tgcgccgttc cgttatgtgg tggaattttt
    37741 ctactggatg ggtggcaact caatggacat tcacagctgg catcggctgg ggatgtggct
    37801 gattggcgcg tttgtgatcg gtcatgtcta catggcgctg cgtgaagaca tcatgtccga
    37861 cgacacggtg atctccacca tggtcaacgg ctaccgtagc cacaaatttg gcaaaataag
    37921 taacaaggag cgttcatgag cgagcaacgc gtggtggtca tggggctggg caacctgctg
    37981 tgggccgatg aaggcttcgg cgtgcgggtg gcggaacggc tgtatgccca ttaccactgg
    38041 cccgagtatg tggagattgt cgatggcggt actcagggac tgaacttgct ggggtatgtc
    38101 gaaagcgcca gccatctgtt gattctcgat gccattgact acgggctgga acctggaacg
    38161 ctgcgaacct atgccggaga acgcattccg gcttatctca gcgcgaagaa aatgagcctg
    38221 catcagaaca gtttctccga agtgttggcg ctggcggata tccgcggaca tctgccagca
    38281 catattgccc tcgtcggtct gcaacccgca atgctcgacg actacggcgg tagcctgagc
    38341 gaactggcac gggagcaact gcccgctgcg gaacaggcgg cgctggcgca gctggctgcg
    38401 tggggaattg tgccgcaacc ggctaatgaa tcgcgctgtc tcaattatga ctgtctgtcg
    38461 atggaaaatt acgaaggcgt tcgcttgcgc cagtaccgga tgacacagga ggagcaggga
    38521 tgagcaacga cacgccattt gatgcgttgt ggcaacgaat gctggcgcgc ggctggacgc
    38581 cagtcagtga atcccgtctt gacgactggc ttacgcaagc gccagacggc gtgctgttat
    38641 taagcagtga cccgaaacgc acgccagagg tcagcgataa tccggtaatg attggcgaat
    38701 tactgcgcga gtttcccgac tatacatggc aggtggcgat tgctgacctt gagcagagcg
    38761 aagccatcgg cgatcgcttt ggcgtctttc gctttcctgc cactttagtg tttaccggcg
    38821 gaaactatcg cggcgtgctg aatggtattc acccgtgggc ggaactgata aacctgatgc
    38881 gcgggcttgt cgaaccgcag caggagcgtg cctcatgagc gaaacatttt tccatctgct
    38941 ggggccagga acgcaaccga acgatgacag tttcagcatg aatccactgc cgatcacctg
    39001 tcaggtgaat gatgaaccga gtatggcggc cctggagcaa tgtgctcaca gcccgcaggt
    39061 gattgcgctg ttaaacgagt tacaacatca actaagcgaa cgccaaccgc cgttgggcga
    39121 ggtgctggca gtcgatctgt taaatctcaa cgccgacgat cgtcacttta tcaatacgct
    39181 tctcggggaa ggggaagtgt cagtgcgcat tcagcaggct gacgacagtg aaagtgaaat
    39241 acaggaggcg atcttctgcg gattatggcg ggtgcgcaga cgtcgcggcg aaaagttgct
    39301 ggaggacaaa ctggaggctg gctgcgcgcc gctggcgttg tggcaggcgg caacgcaaaa
    39361 tctcttgccg acagattcgc tgttaccgcc gcccattgat ggcctgatga atggcctacc
    39421 gttggcgcat gagttactgg cacatgtacg taaccccgac gcgcagccgc acagcattaa
    39481 tctgacgcaa ttacccatca gcgaggctga tcggcttttt ctctcacgtc tctgtgggcc
    39541 gggaaatatt cagattcgta ccattggcta tggcgagagc tatatcaacg ccacggggtt
    39601 acgccatgtc tggcatttac gctgtacgga caccttaaaa ggcccgttac tggaaagtta
    39661 tgaaatctgc ccaataccgg aagtggtgct ggcagcgcca gaagatttgg tcgactctgc
    39721 gcagcggctt agcgaggtat gtcagtggct ggcggaagct gcaccgacgt aaaaagacgg
    39781 taagtatcgc tttcagtctt atgaatatcg caatcggcga atacctctgg tcgtagagtt
    39841 tcaggataaa gagggagatc taccattatc gggttatttt tctctcttcg cctacaggag
    39901 tgcgcatgtg ggatgtcatt gatttatcgc gctggcagtt tgctctgacc gcgctgtatc
    39961 actttttatt tgtacccctt accctggggc tgattttttt gctggctatc acggaaacca
    40021 tttacgtggt caccggcaaa acaatctacc gcgatatgac gcgcttctgg ggtaagctct
    40081 tcggtatcaa ttttgctctt ggcgtggcta ccggcctgac catggagttt cagtttggta
    40141 ctaactggtc attctattcc aactatgtgg gcgatatttt tggcgcacca ctggcgatgg
    40201 aagcattaat ggccttcttc ctcgaatcca cctttgtcgg gctgttcttc ttcggctggc
    40261 aacggctgaa taaataccag cacctgctgg tgacgtggct ggtggcgttc ggttcaaatc
    40321 tctctgcgtt gtggatattg aatgccaacg gttggatgca atacccgacc ggtgcgcatt
    40381 ttgatatcga caccctgcgt atggagatga ccagcttcag cgagctggtc tttaacccgg
    40441 tcagccaggt gaaatttgtg cacaccgtaa tggcgggcta cgtgaccggg gccatgttta
    40501 ttatggcgat cagcgcctgg tatttactgc gcggacggga gcgcgatgtc gcattacgtt
    40561 cgtttgccat cggttccgtc ttcggtactc tggcgattat cggtaccctg caactcggag
    40621 acagttctgc gtatgaagtc gcgcaagtac aaccggtaaa actggcggcg atggaagggg
    40681 agtggcaaac ggaacctgca cctgcaccgt tccatgtggt tgcctggccg gaacaggatc
    40741 aagagcgtaa cgcctttgcc atcaaaattc ccgcgctgct agggatcctc gccactcact
    40801 cattagataa acccgtgccg ggtctgaaga atttgatggc tgaaacctac ccacgcttgc
    40861 aacgcggacg tatggcctgg ctgttaatgc aggaaatatc gcaaggcaat cgtgagccgc
    40921 atgtgttgca ggcattccgg gaactggaag gtgacctggg ctacggcatg ttgctctccc
    40981 gctatgcgcc ggatatgaat catgtcacag ccgcacagta ccaggcggcg atgcgtggcg
    41041 cgatacctca ggttgcgccg gtattctgga gtttccgcat catggtgggc tgtggttccc
    41101 tgctgctact ggtgatgctg attgcgcttg tccagacgct gcgtggcaaa atcgaccagc
    41161 atcgctgggt gctgaaaatg gcgctctgga gtttgccgtt gccgtggatt gcgattgaag
    41221 ccgggtggtt tatgaccgag tttggtcgtc agccgtgggc gatacaggac atcttaccga
    41281 catactccgc gcactccgct ttaaccacag gacaactggc tttctcactg atcatgatcg
    41341 tagggcttta caccctgttc ttaatcgccg aagtctacct gatgcagaaa tatgcccgtc
    41401 tggggccgag cgcgatgcag agtgaacaac cgacgcagca acaggggtaa aggagaaaat
    41461 catgtttgat tatgaaacat tgcgcttcat ctggtggctg ctgattggcg tgatcctggt
    41521 ggtctttatg atctccgacg gatttgacat ggggatcggc tgtctgctgc cgctggtggc
    41581 gcgtaatgat gatgaacgcc ggatagtgat aaacagcgtt ggtgcacact gggaaggcaa
    41641 ccaggtctgg ttgatcctcg ctggtggggc attatttgcc gcctggccca gagtgtatgc
    41701 agcggcgttt tccggctttt atgtggcgat gatcctggtg ctgtgctcac tgttcttccg
    41761 cccgctggcc tttgattatc gcggaaaaat cgccgatgca cgctggcgta aaatgtggga
    41821 cgccggtctg gtcatcggca gtctggtgcc gccggtagtc ttcggtatcg ccttcggcaa
    41881 cttgttgctc ggcgtgccgt ttgccttcac accgcaatta cgcgtggagt atcttggcag
    41941 cttctggcaa ctgctgacgc cattcccttt attgtgcgga ttgctcagcc ttgggatggt
    42001 gattttgcaa ggtggcgtct ggttacaact gaaaactgtt ggtgtgattc atctgcgttc
    42061 acagctggcc accaaacgcg ctgcactgtt ggtgatgctg tgctttttgc tggcgggtta
    42121 ctggctgtgg gtcggtattg atggctttgt actgctcgcc caggatgcta acggtccttc
    42181 caatccgtta atgaaactgg tggcagtgct acctggtgcc tggatgaata atttcgtcga
    42241 atcgcccgtt ttgtggatct tcccgctgct ggggttcttc tgcccattgc tgacggtgat
    42301 ggcgatttat cgtggtcgcc cgggttgggg atttttaatg gcgtcattga tgcaatttgg
    42361 cgtgattttc acggcaggca tcacgctgtt cccctttgtc atgccgtcaa gcgtgagtcc
    42421 aatctccagc ctgacgttgt gggacagcac ttccagtcag ctgacgctga gcattatgtt
    42481 ggtaatcgtg ctgatatttt tgcccattgt gttgctctac actctctgga gctactacaa
    42541 aatgtgggga cgcatgacaa cagaaactct ccgccgtaac gaaaacgagt tgtactaagg
    42601 agcaggaaca atgtggtatt tactttggtt cgtcggcatt ttgttgatgt gttcgctctc
    42661 cacccttgtg ttggtatggc tggacccgcg tctgaaaagt taacgaacgt aggcctgatg
    42721 cggcgcatta gcatcgcatc aggcaatcaa taatgtcaga tatgaaaagc ggaaacatat
    42781 cgatgaaagc gatcttaatc ccatttttat ctcttctgat tccgttaacc ccgcaatctg
    42841 cattcgctca gagtgagccg gagctgaagc tggaaagtgt ggtgattgtc agtcgtcatg
    42901 gtgtgcgtgc tccaaccaag gccacgcaac tgatgcagga tgtcacccca gacgcatggc
    42961 caacctggcc ggtaaaactg ggttggctga caccgcgcgg tggtgagcta atcgcctatc
    43021 tcggacatta ccaacgccag cgtctggtag ccgacggatt gcgggcgaaa aagggctgcc
    43081 cgcagtctgg tcaggtcgcg attattgctg atgtcgacga gcgtacccgt aaaacaggcg
    43141 aagccttcgc cgccgggctg gcacctgact gtgcaataac cgtacatacc caggcagata
    43201 cgtccagtcc cgatccgtta tttaatcctc taaaaactgg cgtttgccaa ctggataacg
    43261 cgaacgtgac tgacgcgatc ctcagcaggg caggagggtc aattgctgac tttaccgggc
    43321 atcggcaaac ggcgtttcgc gaactggaac gggtgcttaa ttttccgcaa tcaaacttgt
    43381 gccttaaacg tgagaaacag gacgaaagct gttcattaac gcaggcatta ccatcggaac
    43441 tcaaggtgag cgccgacaat gtctcattaa ccggtgcggt aagcctcgca tcaatgctga
    43501 cggagatatt tctcctgcaa caagcacagg gaatgccgga gccggggtgg ggaaggatca
    43561 ccgattcaca ccagtggaac accttgctaa gtttgcataa cgcgcaattt tatttgctac
    43621 aacgcacgcc agaggttgcc cgcagccgcg ccaccccgtt attggatttg atcatggcag
    43681 cgttgacgcc ccatccaccg caaaaacagg cagatggtgt gacattaccc acttcagtgc
    43741 tgtttatcgc cggacacgat actaatctgg caaatctcgg cggcgcactg gagctcaact
    43801 ggacgcttcc cggtcagccg gataacacgc cgccaggtgg tgaactggtg tttgaacgct
    43861 ggcgtcggct aagcgataac agccagtgga ttcaggtttc gctggtcttc cagactttac
    43921 agcagatgcg tgataaaacg ccgctgtcat taaatacgcc gcccggagag gtgaaactga
    43981 ccctggcagg atgtgaagag cgaaatgcgc agggcatgtg ttcgttggca ggttttacgc
    44041 aaatcgtgaa tgaagcacgc ataccggcgt gcagtttgta atgcataaaa aagagcattc
    44101 agttacctga atgctctgag gctgatgaca aacgaagaac tgtctaatgc gtagaccgga
    44161 aaaggcgttc acgccgcatc cggccacttt cagttttact ctttctcgga gtaactataa
    44221 ccgtaatagt tatagccgta actgtaagcg gtgctggcgc gtttaatcac accattgagg
    44281 atagcgcctt taatattgac gcctgcctgt tccagacgct gcattgacaa actcacctct
    44341 ttggcggtgt tcaagccaaa acgcgcaacc agcaggctgg tgccaacaga acgccccacg
    44401 accgcggcat cactcaccgc cagcatcggc ggcgtatcga caatcaccag atcgtaatgg
    44461 tcgttcgccc attccagtaa ttgacgcatc cgatcgcgca tcagcagttc agacgggtta
    44521 ggtggcacct gaccgcgagt aatcacatca aagcctcctt tgccaaaatg ctggatcact
    44581 ttgttgagct catctttacc tgccagatat tccgacaagc catgttcatt actcacggta
    44641 aacaggttat gcgaataacc acggcgtaag tcggcatcaa taaataacac tttttgatcg
    44701 gactgggcga tcaccgctgc cagagttgaa ctgacaaacg ttttaccact gtctggcgtc
    44761 gcaccggtga tcatcagaat gttattctcc gtctccatca tagcgaaatg cagactggtt
    44821 cgtagcgcac gtacggcttc cacagcagaa tccgccgggt tatccaccgc caggaagggg
    44881 atatttttag tacgatggcg ctgctgatta gaaaataaat ttttcttacg cagacgggtg
    44941 cgtttatcca gccactcgga cattgggata gtggcataaa cgctgatgcc gtgctcttcc
    45001 agttgttccg gggcttctac accacgacgc aacatcgcac gcgccagcac ggcacccaca
    45061 gaaataaaca ggccaagaat aaaaccaagc accacattca acgctttttt cggtttcact
    45121 ggctgcggct gagtgactgc cgggtcgata atccgcacgt taccaatggc actggatttc
    45181 gaaatactca actcctgctg gcggttaagt aattgcagat ataccgcacg gcccgcttct
    45241 acgtcacgac ttaaacgcaa cacttcctgt tgggtggaag gcattgccga tacccgctta
    45301 ttcaggcgtt tgcgttcttg ctccagcgtc tggcgttttt ccagcagcgc acgataagtt
    45361 gggtgatctt tcttatacag ctgggagatc tctgcctcgc ggaaggtcag ctcattgagt
    45421 tgattatcaa cgttcacaat ctgctcaaga acggctttgg cttccaggtt aaggtcaacc
    45481 gaatcgcgct gctggcgata aacgttgagt ttttcttccg cctggtccag ctcgctgcgc
    45541 acttcaggta actggcgctg taagaattca aggctttgtg aatcctgcgc cgcctggcga
    45601 gcgatattct gttgcaaata gttgttagcg atgctgttca gaatacgagt aatcaactgg
    45661 ggatcatcac cagtcatggt aagttccagc atcccgcttt ctttactgcg ttcgctaacg
    45721 gtaaaggttt cctgcaatgc gttaatcgct tccagttcgg tacgctggct caggacaaac
    45781 tgtgttcctg gtttggcctt aatgtccgcg atagtcagcg caacgccatc tttttccaga
    45841 cgctggccga ccataccatt gacggtgaac tcttcacctt ccagtgtata gtggccgttt
    45901 tccccaaccg tgagtgtcag ttgctgatcc tgaccattca gttgtggaat atgcatccag
    45961 ctgatcgcca gctcacctgg tttttctttg gttaatctcg cccagccgcg acccacaatc
    46021 ggaaaatact tctgctcaac tatgtcgcgc agattcagtt cagcaatggt tttaccgaga
    46081 atcatgcgcg attgcagcag ttggatctcc ggtgcagact cgggcgatga gttagggatc
    46141 atatcgctca ggccgctgag aatggcgttg ccctgttttt gctcaacctg gaccagagta
    46201 tctgcctgat aaattggtgt acttaacagc gagtaagcga cagcgatcag cgtgaataac
    46261 gcggtcacgc tgataataaa cttacggtga tcccataact cgccaaccag acgaagcaga
    46321 tcgatctcat tttcctgagt gctgcctggt ggcgtattca tatttttagt tgtcatcgta
    46381 ttttataaac tcaaattacc ggctgaggcg cttcgcccat tcctgactgg cgcgctccaa
    46441 cataccgtag acatgttcaa atgcgtcctg acttttacga tagggatccg ggatctcttt
    46501 ctgttccagc cattgcccaa acagcattgt cttcccgcgg acctcgggag cgattgcggt
    46561 aacctgagca atatgctctg actccattgc cagaatcaga tcgtaatttc gtgccatctc
    46621 agcagtgagc ttacgtccgg catgtccttc aagagatacg ccatgattag cggcaacatc
    46681 tgctgctgtc gcatctgcag ggtgttttac cagaccatga acacctgccg atttgacttt
    46741 tacgcctggc agacgtttac gcagcaagcg ctcgccaatg ggcgaacggc aaatgttgcc
    46801 ggtacaaacc accaggattg agttaaattt tagttgggcc atgtttgaat ccaccgtgaa
    46861 gtctctgtca ggtcatgcac cccggaaatc gtcggtacca gcagagaaat cacacggttc
    46921 caacgagcca gcggcgcagt agtgacataa acaatgtcgt aaggttcaag ctggaattcc
    46981 gttccgagta tcattgccga tgcgtccttc gcattcagct gataaatatt ggcgattttg
    47041 ccattctgct tgttttgcgt tgcacgaata acaaaaatcc ccgtcgcatc agcgacatcc
    47101 tgattcatac cctcagcgtt acccagcgct tccgccaggg tcataccgct gcggtccatt
    47161 ttcagcgtgc tctgtttacc cacttcaccc atcacgaaga ctttcagatc atcgttgcgc
    47221 ggaataaata aaatatcgcc cgggtgcagc agtttgttct ggcgcaagtc gccacgctgc
    47281 atcagcgcat agaggttcac tttggtcttt acgccattct gggtgagcac cacgttgcgc
    47341 cagtctgcat ctgcggtcag accgcccgcg gcgttaatcg catccatgat ggtcagagga
    47401 atattggtaa tcggctgctg acccgatttg ctgacttcgc cggtgacgta cgctttttgc
    47461 gagcggaacg ccgcgacgct gacatcgact tgcgggcttt caatgactga atccagacgc
    47521 gctgtaatct cgttgcgtac ctgggtaagt gttttcccgg ccactttcag gcgaccaata
    47581 taggggtaga aaatcgcacc atcggcgtta acccagttgc cggtatcact ggcgctacgg
    47641 tattgccctg cgggggtggt tagttccggg tgatcccaga ccgtaaccat caacacatcc
    47701 ccaatgccga tacgatattc atagtttgcg atttgctgct ccagctccgg gttagcctgc
    47761 gacataatcg gtttggcgcg caactgatca atcaatcctg gcgtgacggg atagatattg
    47821 accattttgt ccaggtcata ctgattgtca ggtagttcaa tgacatcctt gttactggtc
    47881 gataaattct gaccaggaac aagggtacat gctgtcaaag acagaacagc caatgttagt
    47941 accgaaaact taaaaatgtt ctttttcatt ggtttatatt tatcagtcaa tgtaatgtta
    48001 tgaattacgt caggttattg tccaaccggt acttctcgct ctgccgtcat tggatagagc
    48061 tggtatttgc gtgacagcat ttgtccacca tcacgcgtca atggtgtcca cgaaaccacc
    48121 gcgcggttgc ggttaggtcc aatggtcatc aagtcgaatg gaatcgagat ataaaaacct
    48181 ttactaaagc cgccttcgcc gtagtcatct ttagatacgt tactgattgc cgcccatacc
    48241 cctaccgcca cgccgctgtc aaagcgtttg gcgacgtcga tcgttgcgcc tttatctttt
    48301 gccagatatt gcccaacgct aagtttcatc agtacgccat tgagcgtcgg cgggttccag
    48361 taagccgtca cgaaacctgt tggcgtggaa taatcggtga aacgcatcat gttgtcccag
    48421 tcacgctgct tcacgtagtt aacgtccaca cccagcgccc agctggcatc tagcgggcga
    48481 taaagcagct cggaaccgac accggcatac atcgtttcca gataaccgcc atacacctgg
    48541 ccatagaaac cattgcctaa gtcagcaaaa tagttcgcct gcaagttgtt gagataaacg
    48601 tcattgcgaa cgtagtcacg gatatgcgtg cgcacgcggg gcagggtaga gtccgcgggc
    48661 aacagcgaag acttaaactt gtcgtagttg ttgtaaatat tggtgaaaat accgccatca
    48721 agcagcaggt ggtcggtaaa ccagtagcgg gcactggaca tcagccccag ctggaacata
    48781 tagaaatctt ccgggccgcc cagcgactgt gaaagtgttg ggttgaaaga gtagctaaag
    48841 cgatcttcac gaatacggta gccccgacca aaggcagaaa gatcttctgc ttcaacacgt
    48901 tgttgttgaa gttgctctga ttgaccaggc gctgtaccag ccagctgttt gcgcaggctg
    48961 gctacgtcgg tttcggtagt caccatcgcc atatgctcgc gcttttgcgt cacgctaatc
    49021 ttctcaacgc cttgcggcag gttattcacc agaatccggt tggcacgatc gaccgcttcg
    49081 cgagaatcac ggtatttgta ttgttgacca gacatataca gcgtcttatc gcgcagctga
    49141 atttccggtg cttcaaaacc tgcgttgtac ttcagcgcgg taagttggtt cgctactgtg
    49201 gtgtactgca atccttcaga ttcaggcgca ggttgatatg ccggttttgg cgtatcgcgc
    49261 agggcagggc gcagatcgtt gaaattggtc cgtaacgtga agccaaacat caacgtgtta
    49321 ccgcgttcat aactcaggtt gagatctgcc cagctggcag cgcgataaac tgcgccgacg
    49381 ttgaaatggc ttgcctgagg cagtttgcca gcgaaatcat tctggtaatt gttgccatcg
    49441 tattcgagtt tcagacgcag gggattccac ggcgtttgat actcaatgcc gccaaagatg
    49501 gaagccgggc cacgaaagat atcgctaaag ctgatatcgc ccgcatcgtg agactctgcg
    49561 cgatgacaat atttatcgct cacccggcaa aacgggttgg taatattgcc cgcattaccg
    49621 gcgtagcccc atgccatccc gagggtgaaa tcaaatggcc ccgcttgctt gctggccacc
    49681 agatactcac cgtcaaacag acccgtacca gcaatatcac gtttaccaaa cgccacttgc
    49741 ggtagccagt aaccttcttc ccacaggcga agtttaaaat cgaatgattt gtctttgtat
    49801 gactgatcgc cgctgaaatc ttcccactgg ctatatttgc gtgtgcgcac atccgtataa
    49861 cgaatggtgc cttccagcca tgggaacagc gccacggagg tggagtagaa ccggtattga
    49921 tcgttatccc ggtagttgac gctgaattca ccttccggtg cgatgcgcgc atttggcatc
    49981 tgcaacaatc ctgtgccgcc aaagtctgat tgcgacgaac ccagcggatc cgggtaggtt
    50041 aatacttcag catgacaggc actggagacg gcaatggcca ggcagcttaa aagataagaa
    50101 ttcttcttca tcagtcagga acacgttgcg ttaaaacaga cacaatctgg tcatttaaat
    50161 cagcatattt ttcgggcagt acatgcgcag aaaagcccaa ccaaagctgg ctgccaggcg
    50221 gtggttccac atgtctttta ttccacaggg caacaggggc gacgacagtc tcaccctcag
    50281 gcgtaatcac cataacgtta ttcttgtccg cgcctgcaag gcgggggtga tcttgcagat
    50341 aatcggtaac gctacggcct gcctgccagg gcaattgccc agccccagaa accgcaccca
    50401 gcagagtaat agtcaccgga cgctgtacgg tatagagcgt atagtccccg accaacgggg
    50461 gattgctgtt ctcatccact cgaacaaaat cagggtccag tttgaccggt aagcgaccag
    50521 tgatattgag attgagtaac tgctggcgca cggatttaat cgttgccgcc acatcgtcgt
    50581 cagcttctgc ttcccaggaa gcaagctgtg ccatgacatg ttggtagtct ttaagcgctt
    50641 tagctttcgc cgcagagtcg gtcagcaagg caccaggcca ccagaggcga tcgcgcagtt
    50701 gcggttgtgt caccaactgg acaacatttt ccacggggcc aacggaaagt gtctgttgtt
    50761 cgccaggcag ataaatagtc accgttcctt gcgcaaaggc atggggtgtc attacgtaaa
    50821 gtacgctggc aatgaaatac gactgtaatt tattcatggt gcgggtttaa gaaacgtcat
    50881 ttctacggga ataacccctg cgcctaacat ttgccgactt tgacgaacct ggccagtagc
    50941 cgaatcgacc cagaatgtgt tttgccactg tttttctggc cgcgtggaag tcacctcttc
    51001 ctgccagaca ttacacagca cattgcgacc cgcaatattc agcgtctcta atccagcaaa
    51061 ggaaaaagta gaactcaggg tcgcagagcg aaaatggttg tcttcactcc agagaatatc
    51121 gcgcgtccag cggctgccat cttttattgc cagcgcgtta cgcagagggt cctgcccgga
    51181 atttgtcact tccagcagat tattattaag tttgacggtt tttaaaagac gtccattgtg
    51241 ggtaaccagc atggcgttat cctgggataa ccatttgctt tgttcttgtt ctatatatcc
    51301 cagtaccaca aagattcgtt gcccttcatt aaggcgtaaa tacatcgtgg aataaggcaa
    51361 cgcctggatc tgctgatccg ctacggtgat atcctgatta tcgaaaaggc tggcgcgaaa
    51421 tgtatcgacc atactttgct ggctgtgcgt acatcccgca agaaatagtg cgaaaatcga
    51481 taaaataaga gggcgcacct ttaatccttg aaaggttaat taaaaaaatg caaatctcta
    51541 aagcccggta cgaatatcgc aaccgggtat catttaactt taatttcgac gcgttaaagc
    51601 agtattagcg tgtagtggac gtggtggttg aggtgttatg agaaccgcca tcgccactgt
    51661 catttgctgc aacaactgcc aacgtcgcca caccaacagc cgttgccaca ccgacaactg
    51721 tcgccgtgcc gacagcgcca gcagccacgc caccagtggt agtagcagtc gtggttgcgc
    51781 cggtagtggt cgttgtggtg ccagtagtac ccgtcgttgc ctcggtcccg tttgttgctt
    51841 caggggcagc aaacgcagct ggcgtcgtca acatgaaggc cataaggatt gcagaaagct
    51901 tgtgtttcat aacttttcct ttattcatcg catggacaat acgtacgctt gagatcagga
    51961 ttgtctactt tgagtataac atccttgtga aatctccctt tcctcatacc gcttgcacta
    52021 tatactgcgg gtaggaaagg cgcgcagagg gaaatataag attgttaact atccccctct
    52081 ggaaatacga cttgtgagca taagataaat cttagcaaaa tacaaattaa agataaggaa
    52141 attgttgcga aagctattaa ttattgttgg taatatagtt tcaagtggta ctataactgg
    52201 ctgttaaatg gcatttataa ctattaggtg cagagatatt cgcttaaatg gattagtttc
    52261 tttgtggaaa agtaactgat gttataatgt atgatgttga ctattgcact attccaataa
    52321 aaccagtcag ctttaaacaa gcagcgtcat attaagagag ataaacattt gccgctgttg
    52381 gtcctcgcag gccatttacg cggcaaaatt ccacacgtaa tcctggtata agcacttctg
    52441 cgtcgcgggg agtgaatgcg gaaatatgga cctgaacttc tttacgaccg tcggagggga
    52501 taatgaatcc tttgccgctt ttgcgatcaa aggttttgac aattcctgtc attttacggg
    52561 acaaacaaat tccttactga aaatactgcg ctgcactata cggggttaat aaaataaagc
    52621 cagcgatatt taagaccgcc ggacggctaa aataaaattt gcttaatctc aattatcatg
    52681 cgttaatagc tgcgtcggtt tgaaagacag acagcataca aagtagttta ctaaagcagt
    52741 tctcattatc aggcattatc cccttctttt gagtctctct cctgaacact aagtagtttc
    52801 tgtattaaag ccctgtttgc cgaaaggccc aaaatgaagg aagtaaaata tgtctaataa
    52861 aatgactggt ttagtaaaat ggtttaacgc agataaaggt tttggcttta tcactcctga
    52921 tgatggcagc aaagacgttt tcgtccattt caccgccatc cagagcaatg aattccgcac
    52981 gctgaacgaa aatcagaaag ttgaattttc tattgagcag gggcaacgtg gccccgcggc
    53041 agcgaacgtt gttacgctct aaggttgccg ttattactca acatctccat ttccgctgtc
    53101 catgttgtca tggttcacag taccgcacat cggcattcga tgtgacggag cgaaaccctt
    53161 tgggcgctaa gtgtattttt tgtaaatcga cgatgatcac ctttgataac gtcgcgctgc
    53221 aaatatgcac ggaccatgcg ccgctggatt tcacaaaata atatcaggct ccctcgtgga
    53281 gcctttttta tatctgcctt atttttcttc aacgctgtat gtatagtaag cgataacctg
    53341 ttgattattg aatctttcgg ggagatggct tataacattt cttacctgac cagggtaccg
    53401 ggaaccaaca ccttactggc gtgttgctgt ctttcaagac cagaagaggt taacagtgaa
    53461 tattgaagag ttaaaaaaac aagccgaaac ggaaatcgcc gactttatcg cgcaaaaaat
    53521 cgccgagctg aacaagaata cagggaaaga agtctctgaa attcgcttca ccgcacgaga
    53581 aaaaatgacc gggcttgaaa gttatgatgt caaaatcaaa ataatgtgat tttgtgaaca
    53641 tcacccgtgc gaggtgatgt tccgcttgtt gctaatttag tgaccaatca ttggcgcttg
    53701 tggaattaag cgtcggtaca attcatccgg taccgggctt tgccatactc ccgcatacat
    53761 tgcgtaacca atcaccgcaa acataatccc cagaaccagt agcgtcatta accagccaga
    53821 caacgcaaag gcttttttat ttgccgcagg tttttgcagt gaaaaggtca atgttgaggc
    53881 taccggacat gactctacgc aagtcataca gccggtacat tccactgttc gtacctgaat
    53941 taatttatcg accgggatcc gtgatgggca attttttgcg catttgccac agtcgataca
    54001 actttcggca ttgcgacgaa tcttaaacgg cgacaatagc gaaaccacgc ccatcagcgc
    54061 gccatatggg caaagataac gacaccaggc atggcgaata aacaggctgg caatcagcaa
    54121 aacggtcacg ctgattaatg tcgcggtccc catatgacga aagaaatcga gcattttaac
    54181 gtccatcacc acgctgtagg gcgacaacat aaaatagtga atcgcctgag cgggcatcaa
    54241 taacgcgata tagagaaaaa aactcaacag caaatacttc acgccgcgca gaggaatatc
    54301 cagccagcgg ggaaggacac attgccgacc aaacagtttg ttaccgagat cgccgattaa
    54361 ttcagaaagc gtaccaaccg ggcataacca tgagcaaaag gcctttttga gtaatagact
    54421 gatgacgata aaagcgacca ataacagcat cgcggcggcg tggacggacg gtaactgacc
    54481 tgttacaagg ctatatttca gattcatcag cccggcaatc ggtagccagc cttcgatacc
    54541 tcccggtctg gcgacaaatg tcgtgctact tgccgtttcg taatagcgca cccaatacca
    54601 gaacgtgatg gcaatataaa tattcgttgc caacagtaat aattgcgtcg ctttacgcca
    54661 ggtcgtggca ttacgccagt cattccacgg taatttgccg cccgtcgtgc ctggccgccg
    54721 ctgccagcgg gttcttttat tctctgccat gattttgcca gtccgttaag ttgtatacca
    54781 aatgccacta ttctagttgt tcttaactgg ctgatattga ttcaaatcgc gttcaggtct
    54841 ttcttatgca accatgcttc cagagcggca acactgcgtg taatttcttc gtgtggaagg
    54901 ggggcagata atggctgctg ctccagttgt gcgcatagct ggctggcgat acgcattccc
    54961 agactcgagc aactgctttt tagctgatgc gcggcacgct ttattttctc gctatcctga
    55021 ctggcgcggg caatgtcgat ttcatcgaga agcggcaggg catgttgtgt aaataatacc
    55081 agccattcgt ggatcttctc cgtccccatt aactgagcat cttcattgag ttgcgataca
    55141 tccagcgatt gatcgttatt gacttgcagt tggagatagt gcgccagtaa ctgaccgagc
    55201 acttcacgcg gcaccggttt agggataatc ccgcggaata atgaactggt acgctggcgc
    55261 agcgtttcgt caatgacatg ggcgctaaag ccaatcaaaa ccagcgacgg atattgctgt
    55321 gccagttgtc gggcaagcgt aatgccgtcg acatccggca gatcaaaatc caccagtgcg
    55381 gcagcaaacg gttcgctatt ttgcagtgtc tctaaagcct gcgcggcatt gccaacagca
    55441 acaacctgcg caccactggt gttcagcatc tcaacggtaa ttcgctgggt tagcgggtta
    55501 tcttcaatta acagcaaacg taaaccgtca agacgcaccg cctgattgac tgtttttggc
    55561 acgggtgccg tggcaacacg taacggcaag cgtaaacaaa aacagcttcc aacctccggc
    55621 gtgctggtgg cgctcagttc gccgcccatc gcctgggcca gacggctact gatagtcagc
    55681 cccagcccgg tgccgccgcg tttgccgctt acctgcacaa atggctggaa gatttctgcc
    55741 agtttcgcgg gatcaatacc gcagccgctg tcttccactt cgaccagcca ttgctcacca
    55801 tcagtgcgac tacgcaggat aatgtaccct tcgtcagtaa aacgcagggc gttgctcaac
    55861 aggttggtta taacctgacg aatacgtcgt ggatcgccca ttaacgcgca cggcatatca
    55921 tcggcaattg ccgttgccag gcgaatcggg cgacctttca cccgtccgct cattaattgc
    55981 agggtacttt ccagcagcgg gcgcggttca aagggctcat cgctgaccga aacattcttg
    56041 ccacctgctt cgatagcgga ataatcgaga atatcgttga ggatggtcag caacgattca
    56101 ccagagtcag taattgcccg caaatcatca cgctgggcgt taagtgcggg gttatctgcc
    56161 agcagttgag cagtgccgag aataccgtac agcggtgtgc ggatctcatg gctcatcgcc
    56221 gccagaaacg ccgattttgc ctggctggct ttttctgctt ccgcccgtgc ctgtcggtgt
    56281 tctatcacca gttcctgcaa ttcagctgta cgcgctttga cctgcgccgc cagctgttcg
    56341 cggtggcgat tcagtgcatg aacattgctg cgaaacgcat ccatcagccg cccgatggta
    56401 tccagctccc gtacgccagc ggtttccggg aaaggggagt caatatcacc gtccagcagc
    56461 cgttgcagcg cctgcgtttg ttcggcaagt ggacgcgtga ctgagcgata aaccacgcgc
    56521 caaaggatca gaatcagtgc gcaaagtgaa accatcccca gcaataacag gctgtattgc
    56581 ccgcgtgcac tggctttttc cagatgtgcc agtccatgct gattgcgcag ctcaatggtg
    56641 tcgaccagct gactgacttc gctactaaac tgcgcgaact gggcgatatt attttgggcg
    56701 agagtttgca ggtgattgct gatttcactg tcctgctgga acagcgccaa cagatcgcta
    56761 tattggctaa cggtagttaa cgttgttgcg acctgcgcac gaacacccgg atcttcaatg
    56821 cgtatttgcc gacgttgcag aattttcacc gcattattga gctgcttttc cagcgttggc
    56881 gcatttttct ggatctgctc cagccccaga ttcatcacca tttgctgcac ccgcagggcg
    56941 ctaaggcgca gttcattcat ctggttaaca tactcaagat cgatatcaat cagccgatcg
    57001 agtgcacttt ctgcggcctg acgctgatct tgttcgatca aatcgtaaat cccggcctgg
    57061 gtcgctccgg cggaggttgc cgcattattc gcctgacctt gcgccaggcg tgcgatctca
    57121 tcggcggcag cgactatctg ctgactgagt tgctgttgtt gctggcgcag ttgcaaacgc
    57181 tgccccacca gttccccttg ctgacgtaac gaacgggaga tctcctgctc ctgttgttca
    57241 atagcggtgg tatcaaaacc ttgttcccgt aacgcttgca gcaacgcatt aatcttcagg
    57301 ctttgtgcgg tgagcatttg cccctgcgcc tgccacatct tttcgttatc ggcactggtc
    57361 aggttctgcg cggcgaaaag ttcccaggcg ctggcttcgc tcaactggcg cgccatattc
    57421 atggtaggaa tcaatgcctg agtattgtct ttttccacct ggctgataaa gcgcaggttg
    57481 taccatccca ccagggtact ggtcagggtt aacagcgcca tcagggcaaa gcccatccag
    57541 agtcttcggg tcagggttaa attcacggtc ggtgcacttt aggtgaaaaa ggttgagtcg
    57601 caaagcggaa tgcatctagc ataaagcctt attattgatg aggctatcat gcgcgtactg
    57661 ctatttttac ttctttccct tttcatgttg ccggcatttt cggctgataa cctgttgcgc
    57721 tggcatgatg cgcagcattt cacggtgcaa gcctctacgc cgcttaaagc caaacgcgca
    57781 tggaaactgt gcgcgcttta tcccagcctg aaagattcat attggttatc gttgaactat
    57841 ggtatgcagg aggctgctcg ccgctacggt gtggatttaa aagtgctgga ggcaggcggc
    57901 tacagccagt tggctaccca gcaagcacaa atcgaccagt gtaaacagtg gggcgcagag
    57961 gccattttgc tcggtagtag cacgacctca tttcccgacc tgcaaaagca ggtagcaagt
    58021 ctgccggtga tcgaactggt aaatgctatt gatgctcccc aggtgaaaag ccgcgttggt
    58081 gtgccctggt ttcagatggg ctatcaaccg gggcgatatc tggtgcaatg ggcgcacggt
    58141 aaaccactga atgtgctgtt gatgcccgga cccgataacg ccgggggcag taaggagatg
    58201 gtcgagggtt ttcgcgcagc cattgccgga agcccggtgc gtattgttga tattgcgctt
    58261 ggtgataacg atattgaaat ccagcgtaac ctgttgcagg agatgctgga acgccaccca
    58321 gaaatcgacg tcgttgccgg aacggccatt gcggcagagg cggcaatggg tgaagggcgt
    58381 aacctgaaaa cgccgcttac cgtggtgtcg ttttatcttt cacatcaggt gtatcgcggg
    58441 ctgaagcggg gaagagtgat tatggctgcc agcgatcaaa tggtctggca gggggaactg
    58501 gcagttgagc aggccatcag gcaattacag gggcaatcgg tgtctgataa tgtcagccca
    58561 ccgattttag ttctgacgcc gaaaaatgcc gaccgagaac atatccgccg ctcgctgtca
    58621 ccagggggat ttcgtccggt ctatttttat cagcacacat cagcggctaa gaaataacct
    58681 tcaccatgtt gcgtcaccag taaatccgcg ctgagtttat gacgtaaacg acgaattaac
    58741 acatcgacgg tgcgcaggtc agggttttcc acccgacgcg cagaaagcat acgtagcaga
    58801 cgttcacggc tgagaatttc gcccggattc gtcacaaatg ccaccaacat ttcatactct
    58861 gcgcgggtca gtttaatcgg ctcgccatcc cgctccagcg tatggcgcga cacattcagg
    58921 caataaccgg caaagcgata gcagttgtcc tgagtgtgcg gttgagcttg tcgcgcgagg
    58981 tcgattcgcc agagcagatt tttcacccgt actaccagtt cgcgcagttc cagcggtttg
    59041 gtgacgtaat cgtctgcgcc catttccagc ccaacaatac ggtcaatccg atcgctgcgt
    59101 ccggtaacca gaataatccc caccgttgag cgttctcgca gggcgcgggt taacatcagg
    59161 ccattttcat cgggtaagtt gatatccagc agaattaaat ctaccggctg attctgcata
    59221 atttcccgca gcccggcacc gctcgctgta acggaaacgg tatacccctc ctgagtgaag
    59281 taagattgta atcgcgcctg ggtaaccggc tcatcttcaa caataacaat gtgatgtggc
    59341 atcagagggt tttactcatt ctgttcatat ctgttcatat tctgccgtaa gccgttcatc
    59401 ctgaccagtg ccgctgttca tatttgctca ttaagatcgc ttcactaaac cataatttta
    59461 caggggctat tatgcggaaa ctctggaacg cgctacgccg acccagtgct cgttggtcgg
    59521 tactggcgct ggtcgcaatt gggattgtga ttggcattgc gctgattgta ttgccacacg
    59581 ttgggatcaa agtcaccagc acaaccgaat tttgtgtcag ttgccacagt atgcaaccgg
    59641 tgtatgaaga atataaacag tcggtgcatt tccagaacgc ctccggcgtg cgagctgaat
    59701 gccatgactg tcatatcccg ccggatattc caggcatggt gaagcgcaaa ctggaagcga
    59761 gcaatgatat ctaccagacc tttattgctc actccattga tacacctgaa aaattcgaag
    59821 ccaaacgcgc ggaacttgcc gagcgtgaat gggcgcgaat gaaagaaaac aactcggcaa
    59881 cctgccgctc ctgccataac tacgatgcga tggatcatgc gaagcagcat cctgaagcgg
    59941 cacgtcagat gaaggtggca gcgaaagata atcaatcctg catcgactgt cataaaggta
    60001 ttgcccacca gttaccggat atgagtagcg gcttccgtaa gcagttcgat gagctgcgcg
    60061 ccagtgctaa tgacagtggt gacacgctgt actctattga tattaagcca atttatgcgg
    60121 cgaaaggcga taaagaagcc tctggttctc tgctgcctgc ttcggaagtg aaagtcctta
    60181 aacgtgacgg cgactggctg caaattgaaa ttaccggctg gacggaaagc gccggacgtc
    60241 agcgtgtact cacccaattc ccaggtaaac gcatctttgt tgcctcgatt cgtggtgatg
    60301 tgcagcagca ggtaaaaacg ctggagaaaa ccaccgttgc cgacaccaat accgagtgga
    60361 gcaagttgca ggccactgcg tggatgaaga aaggcgacat ggtgaacgat atcaaaccga
    60421 tctgggctta tgcggattcg ttgtacaacg gcacctgtaa ccagtgccac ggcgcaccgg
    60481 aaatcgccca ctttgacgct aacggttgga tcggcacgct caacggcatg attggcttta
    60541 ccagtctcga taaacgtgaa gaacgcacct tgttgaaata tctgcaaatg aatgcgtctg
    60601 acaccgcagg taaggctcac ggcgataaga aggaagaaaa ataatgaaca ataacgatct
    60661 ctttcaggca tcacgtcggc gttttctggc acaactcggc ggcttaaccg tcgccgggat
    60721 gctggggccg tcattgttaa cgccgcgccg tgcgactgcg gcgcaagcgg cgactgacgc
    60781 tgtcatctcg aaagagggca ttcttaccgg gtcgcactgg ggggctatcc gcgcgacggt
    60841 gaaggatggt cgctttgtgg cggcaaaacc gttcgaactg gataaatatc cgtcgaaaat
    60901 gattgccgga ttgccggatc acgtacacaa cgcggcgcgt attcgttatc cgatggtacg
    60961 cgtggactgg ctgcgtaagc gccatctcag cgatacctcc cagcgcggtg ataaccgttt
    61021 tgtgcgcgtg agctgggatg aagccctcga catgttctat gaagaactgg aacgcgtgca
    61081 gaaaactcac gggccgagtg ccttgctgac cgccagtggt tggcaatcga cggggatgtt
    61141 ccataacgct tcggggatgc tggcgaaagc tattgccttg catggtaata gcgttggtac
    61201 gggcggagat tactctaccg gtgctgcgca ggtgatcctg ccgcgcgtgg ttggctcaat
    61261 ggaagtgtat gaacagcaaa cctcctggcc gctggtattg cagaacagca aaaccattgt
    61321 gctgtggggc tctgatttgc tgaaaaacca gcaagcgaac tggtggtgcc cggatcacga
    61381 tgtttatgaa tattacgcgc agctaaaagc gaaagtcgcc gccggtgaaa ttgaggtcat
    61441 cagcatcgat ccggttgtca catccaccca tgagtatctg gggcgcgagc atgtgaagca
    61501 cattgcggtt aacccgcaaa ctgatgtgcc gctgcaactg gcgctggcgt atacgctgta
    61561 cagtgaaaac ctgtacgaca aaaacttcct cgctaactac tgtgtgggtt ttgagcagtt
    61621 cctgccgtac ctgctgggtg agaaagacgg tcagccgaaa gatgccgcat gggctgaaaa
    61681 actgaccggc attgatgccg aaaccattcg tgggctggcg cggcagatgg cggcgaacag
    61741 aacgcaaatt attgctggct ggtgcgtgca gcgtatgcag cacggtgaac agtgggcgtg
    61801 gatgattgtg gttctggcgg cgatgctggg gcaaattggc ctgccaggtg gtggctttgg
    61861 ttttggctgg cactataacg gcgcaggcac gccggggcgt aaaggcgtta ttctgagtgg
    61921 tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgattaca aaggctacag
    61981 cagcaccatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga aagtgatcaa
    62041 ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg ccggaactaa
    62101 cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca agctggaaac
    62161 ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata tcgtgctgcc
    62221 tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact ccaaccgtgg
    62281 cattatcgcc atgaaacagg tggtgccgcc gcagttcgag gcgcgcaacg actttgatat
    62341 tttccgcgag ctgtgccgtc gctttaatcg cgaagaagcc tttaccgaag ggctggacga
    62401 aatgggctgg ctgaaacgca tctggcagga aggtgtacag caaggcaaag gacgcggcgt
    62461 tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt ttgaccatcc
    62521 gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac cgctgggcac
    62581 gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact acgacgattg
    62641 tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc ccggctcgca
    62701 gaagtatccg ttgcatctgc aatctgtgca tccggatttt cgacttcact cgcagttatg
    62761 tgagtcggaa actctgcgtc agcaatatac ggtagcgggt aaagagccag tattcattaa
    62821 cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg tctttaacgc
    62881 tcgcggtcag gtgttggcag gggcagtggt ttctgaccgc tatgcacccg gtgtggcacg
    62941 aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg cgctgtgcaa
    63001 atacggtaac cccaacgtgt tgaccatcga catcggtaca tcgcagctgg cgcaggcgac
    63061 cagtgcgcac actacgctgg tggaaattga gaagtacaac ggaacagtgg agcaggtgac
    63121 ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg cgtcgcaggt
    63181 gaaatcatga ccacgctgac agcacaacag attgcctgta tttacgcctg gctggcgcag
    63241 ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc gcagatggct
    63301 gaatggtttt cgttgctgaa aagcgaaccg ccgctggctg cggcggtgaa tgcgctggaa
    63361 aaccgtattg ccgctctgac agtacgtgac gatgcccgtc tggaactggc cgctgacttt
    63421 tgcggcctgt ttctgatgac cgacaaacaa gcggcgctgc cgtatgcatc ggcctacaaa
    63481 caggacgagc aagagattaa acgcttgtta gttgaggcag ggatggaaac cagcggcaat
    63541 ttcaacgaac cggcagatca tctggcgatc tatctcgagt tgttgagtca tctgcatttt
    63601 tcgctgggag aggggaccgt tcctgcgcga agaatcgaca gtttgcggca aaaaacactg
    63661 acggcgctgt ggcaatggtt accagagttt gttgcgcgtt gccatcagca tgacagcttt
    63721 ggtttttacg cggcactaag ccagttattg ctggtgttag tggagtgcga ccaccaaaac
    63781 agataacgtc gtttgtgcgc ctgaaaagac gcgtttagcg tcgcatcagg cattatgggg
    63841 cagttgccgg atgcgtcatc acatccggca aacacacatc acggatgagt tacaaaccgg
    63901 gaaagtcgct ggcgcagcag gcggttttcc tgcttcaggt gcgcaatatc atccattaac
    63961 gtcagcgcca ccgcgatccc aggccagtcc agagccagtt catgacgcag gcgtactgcg
    64021 cgttgcacca cgatggcggc atggtcgtca aatacccagg ttgtttcctg gatctcacgc
    64081 ggttcaacca cccccaaacc gacaatttca ttcaactcct cttcagagat gccggtatgc
    64141 aggcaaaatt cggtaatagt aaaagtcacc gtaacattag ccattatgct ttcccccaat
    64201 ctttacgtgg atcaaaagac gactgggcgt ctgccagttg ctgccacagc gcggcagtgt
    64261 tttcatccgg tttcggcggc atcacgattt tcagtaccgc atacagatcg ccggtctgtt
    64321 ttttgctcac cagacctttg cctttaacgc gcaatcgttg cccggcctgg ctgcctggcg
    64381 ggatagtcag caaaatgctt tctttcagtg ttggaacggt gactttagca cccagcgccg
    64441 cttcccacgg gctaaccggc actacaattt ccagatcatg gccgacaata tcgaacagcg
    64501 gatgtggcgc aatatgaatc accagccaca aatcgccatt tggaccgccg ttttcgcccg
    64561 gcgtcccctg gcctttcaga cggatgcgtt gaccattgcc gacgcccgcc gggatcttga
    64621 cattcagcgt tttcggaatt tcctgttcga tcatgccaaa ggcgttataa accggcaggt
    64681 tatagctgat ggtacgctta tgctcagtca gcgtttcttc gaggaatacc gccacttcga
    64741 tttcaatatc gtggccgcgt gtggcggggc gttgacggct ctggcgggca tgctgaccga
    64801 aaattgacga gaagatatcg tcaaaatctt cggcgttaaa actctgaccg tcgctatgat
    64861 ggaactgacg gttaaattgc ggatcgttgc gatgttgcca catctgatca tactcagcgc
    64921 gacgttgttc atcacttaac acttcccagg cttcagcgac ctctttgaag cgggcttcgg
    64981 catccggttc tttgctgaca tcaggatggt atttgcgggc cagtcgacga taggcggtct
    65041 tgattgtctt gagatcgtcc gtcggtttca cgcccatgat ggcgtaataa tcctttaatt
    65101 ccatagcgtt atctcgcgta aatcaacaca aattgaagga acccctgtaa ggtaactcct
    65161 ataagtgtag ggtaatcctc aaaatttcat atgccaacac agaatatgtt attgaaatca
    65221 tcgcggagag gaggtcgcct caaggtgggg tgctgaacat attttaaaca ggtgaaaaag
    65281 ggtgagcgat ttttgatagt tgaaccaggc actttaagtt taactggggc gtcattattt
    65341 attaaatttt atagacgcta tatatgggta gtaatataca tggaattagt tgca
//
LOCUS       DAPHDW010000007        62980 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.007, whole genome shotgun sequence.
ACCESSION   DAPHDW010000007 DAPHDW010000000
VERSION     DAPHDW010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 62980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 62980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..62980
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.007"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(164..240)
                     /locus_tag="RH057_000424"
     tRNA            complement(164..240)
                     /locus_tag="RH057_000424"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Arg,seq:acg)
     gene            complement(438..514)
                     /locus_tag="RH057_000425"
     tRNA            complement(438..514)
                     /locus_tag="RH057_000425"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(478..480),aa:Arg,seq:acg)
     gene            complement(518..610)
                     /locus_tag="RH057_000426"
     tRNA            complement(518..610)
                     /locus_tag="RH057_000426"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(574..576),aa:Ser,seq:gct)
     gene            complement(926..1111)
                     /gene="csrA"
                     /locus_tag="RH057_000427"
     CDS             complement(926..1111)
                     /gene="csrA"
                     /locus_tag="RH057_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HDV3126844.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(1346..3976)
                     /gene="alaS"
                     /locus_tag="RH057_000428"
     CDS             complement(1346..3976)
                     /gene="alaS"
                     /locus_tag="RH057_000428"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HDV3126845.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADVDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMDLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(4104..4604)
                     /gene="recX"
                     /locus_tag="RH057_000429"
     CDS             complement(4104..4604)
                     /gene="recX"
                     /locus_tag="RH057_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HDV3126846.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4847..5908)
                     /gene="recA"
                     /locus_tag="RH057_000430"
     CDS             complement(4847..5908)
                     /gene="recA"
                     /locus_tag="RH057_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HDV3126847.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5988..6485)
                     /gene="pncC"
                     /locus_tag="RH057_000431"
     CDS             complement(5988..6485)
                     /gene="pncC"
                     /locus_tag="RH057_000431"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HDV3126848.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6630..7715)
                     /gene="mltB"
                     /locus_tag="RH057_000432"
     CDS             complement(6630..7715)
                     /gene="mltB"
                     /locus_tag="RH057_000432"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HDV3126849.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETDTTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAVMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7971..8534
                     /gene="srlA"
                     /locus_tag="RH057_000433"
                     /gene_synonym="gutA"
     CDS             7971..8534
                     /gene="srlA"
                     /locus_tag="RH057_000433"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HDV3126850.1"
                     /translation="MIETITHGAEWFIGLFQKGGEVFTGMVTGILPLLISLLVIMNAL
                     INFIGQHRIERFAQRCAGNPVSRYLLLPCIGTFVFCNPMTLSLGRFMPEKYKPSYYAA
                     ASYSCHSMNGLFPHINPGELFVYLGIASGLTTLNLPLGPLAVSYLLVGLVTNFFRGWV
                     TDLTTAIFEKKMGIQLEQKVHLAGATS"
     gene            8531..9490
                     /gene="srlE"
                     /locus_tag="RH057_000434"
                     /gene_synonym="gutE"
     CDS             8531..9490
                     /gene="srlE"
                     /locus_tag="RH057_000434"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HDV3126851.1"
                     /translation="MTHIRIEKGTGGWGGPLELKATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            9501..9872
                     /gene="srlB"
                     /locus_tag="RH057_000435"
                     /gene_synonym="gutB"
     CDS             9501..9872
                     /gene="srlB"
                     /locus_tag="RH057_000435"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HDV3126852.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9876..10655
                     /gene="srlD"
                     /locus_tag="RH057_000436"
     CDS             9876..10655
                     /gene="srlD"
                     /locus_tag="RH057_000436"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HDV3126853.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGESMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSPMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10761..11120
                     /gene="gutM"
                     /locus_tag="RH057_000437"
     CDS             10761..11120
                     /gene="gutM"
                     /locus_tag="RH057_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HDV3126854.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVGR
                     SSGRFKPRVVVAIALDDQQRIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            11187..11960
                     /gene="srlR"
                     /locus_tag="RH057_000438"
                     /gene_synonym="gutR"
     CDS             11187..11960
                     /gene="srlR"
                     /locus_tag="RH057_000438"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HDV3126855.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQMVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11953..12918
                     /gene="gutQ"
                     /locus_tag="RH057_000439"
     CDS             11953..12918
                     /gene="gutQ"
                     /locus_tag="RH057_000439"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HDV3126856.1"
                     /translation="MSEALLNAGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSNALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTVGGTTL
                     QSQSRAIDAKEILMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12915..14429)
                     /gene="norR"
                     /locus_tag="RH057_000440"
     CDS             complement(12915..14429)
                     /gene="norR"
                     /locus_tag="RH057_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HDV3126857.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFAGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMMPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRLGLSRVVLSAGAR
                     NLLQHYRFPGNVRELEHAIHRAVVLSRATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGMKD"
     gene            14616..16055
                     /gene="norV"
                     /locus_tag="RH057_000441"
     CDS             14616..16055
                     /gene="norV"
                     /locus_tag="RH057_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HDV3126858.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAVDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFEELASEAK"
     gene            16052..17185
                     /gene="norW"
                     /locus_tag="RH057_000442"
     CDS             16052..17185
                     /gene="norW"
                     /locus_tag="RH057_000442"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HDV3126859.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSMDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAEQFNLHLFPQTWVTDIDAEARVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDRQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSAMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFGLLKTLPM"
     gene            complement(17411..19663)
                     /gene="hypF"
                     /locus_tag="RH057_000443"
     CDS             complement(17411..19663)
                     /gene="hypF"
                     /locus_tag="RH057_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HDV3126860.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSAGGAMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAEFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSTAVATLRARKHRPAKPLAVILPVAEGLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLIHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALALPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYVV
                     HDAHPGYVSSQWAREMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYSE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCHGVTHPVTMPLVDNQLDLATFWQQWLNWQAPVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19816..20343)
                     /gene="hydN"
                     /locus_tag="RH057_000444"
     CDS             complement(19816..20343)
                     /gene="hydN"
                     /locus_tag="RH057_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HDV3126861.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVHVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(20492..21502)
                     /gene="ascG"
                     /locus_tag="RH057_000445"
     CDS             complement(20492..21502)
                     /gene="ascG"
                     /locus_tag="RH057_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HDV3126862.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESG
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRKNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALAQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAMKALHERGVAVPEQVSVIGFD
                     DIAIAPYTVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            21762..23219
                     /gene="ascF"
                     /locus_tag="RH057_000446"
     CDS             21762..23219
                     /gene="ascF"
                     /locus_tag="RH057_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose/arbutin/salicin transporter
                     subunit IIBC"
                     /protein_id="HDV3126863.1"
                     /translation="MAKNYAALARSVIAALGGVDNISAVTHCMTRLRFVIKDDQLIDS
                     PTLKTIPGVLGVVRSDNQCQVIIGNTVSQAFQEVVSLLPGDMQPAQPVGKPKLTLRRI
                     GAGILDALIGTMSPLIPAIIGGSMVKLLAMILEMSGVLTKGSPTLTILNVIGDGAFFF
                     LPLMVAASAAIKFKTNMSLAIAIAGVLVHPSFIELMAKAAQGEHVEFALIPVTAVKYT
                     YTVIPALVMTWCLSYIERWVDRITPAVTKNFLKPMLIVLIAAPLAILLIGPIGIWIGS
                     AISALVYTIHGYLGWLSVAIMGALWPLLVMTGMHRVFTPTIIQTIAETGKEGMVMPSE
                     IGANLSLGGSSLAVAWKTKNPELRQTALAAAASAIMAGISEPALYGVAIRLKRPLIAS
                     LISGFICGAVAGMAGLASHSMAAPGLFTSVQFFDPANPMSIVWVFAVMALAVVLSFIL
                     TLLLGFEDIPVEEAAAEARKHQSAQPTVAKEVSLN"
     gene            23228..24652
                     /gene="ascB"
                     /locus_tag="RH057_000447"
     CDS             23228..24652
                     /gene="ascB"
                     /locus_tag="RH057_000447"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase AscB"
                     /protein_id="HDV3126864.1"
                     /translation="MSVFPEGFLWGGALAANQSEGAFREGGKGLTTVDMIPHGEHRMA
                     VKLGLEKRFQLRDDEFYPSHEATDFYHRYKEDIALMAEMGFKVFRTSIAWSRLFPQGD
                     EITPNQQGIAFYRSVFEECKKYGIEPLVTLCHFDVPMHLVTEYGSWRNRKLVEFFSRY
                     ARTCFEAFDGLVKYWLTFNEINIMLHSPFSGAGLVFEEGENQDQVKYQAAHHQLVASA
                     LATKIAHEVNPQNQVGCMLAGGNFYPYSCKPEDVWAALEKDRENLFFIDVQARGAYPA
                     YSARVFREKGVTINKAPGDDEILKNTVDFVSFSYYASRCASAEMNANNSSAANVVKSL
                     RNPYLQVSDWGWGIDPLGLRITMNMMYDRYQKPLFLVENGLGAKDELAANGEINDDYR
                     ISYLREHIRAMGEAIADGIPLMGYTTWGCIDLVSASTGEMSKRYGFVYVDRDDAGNGT
                     LTRTRKKSFWWYKKVIASNGEDLE"
     gene            complement(24811..25281)
                     /gene="hycI"
                     /locus_tag="RH057_000448"
     CDS             complement(24811..25281)
                     /gene="hycI"
                     /locus_tag="RH057_000448"
                     /EC_number="3.4.23.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation peptidase HycI"
                     /protein_id="HDV3126865.1"
                     /translation="MTDVLLCVGNSMMGDDGAGPLLAEKCAAAPKGNWVVIDGGSAPE
                     NDIVAIRELRPTRLLIVDATDMGLNPGEIRIIDPDDIAEMFMMTTHNMPLNYLIDQLK
                     EDIGEVIFLGIQPDIVGFYYPMTQPIKDAVETVYQRLEGWEGNGGFAQLAVEVE"
     gene            complement(25274..25684)
                     /gene="hycH"
                     /locus_tag="RH057_000449"
     CDS             complement(25274..25684)
                     /gene="hycH"
                     /locus_tag="RH057_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase assembly protein HycH"
                     /protein_id="HDV3126866.1"
                     /translation="MSEKVVFSQLSRKFIDENDATPAEAQQVVYYSLAIGHHLGVIDC
                     LEAALTCPWDEYLAWIATLEAGSEARRKMEGVPKYGEIVIDINHVPMLANAFDKARAA
                     QTSQQQEWSTMLLSMLHDIHQENAIYLMVRRLRD"
     gene            complement(25681..26448)
                     /gene="hycG"
                     /locus_tag="RH057_000450"
     CDS             complement(25681..26448)
                     /gene="hycG"
                     /locus_tag="RH057_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycG"
                     /protein_id="HDV3126867.1"
                     /translation="MSNLLGPRDANGIPVPMTVDESIASMKASLLKKIKRSAYVYRVD
                     CGGCNGCEIEIFATLSPLFDAERFGIKVVPSPRHADILLFTGAVTRAMRSPALRAWQS
                     APDPKICISYGACGNSGGIFHDLYCVWGGTDKIVPVDVYIPGCPPTPAATLYGFAMAL
                     GLLEQKIHARGPGEQDEQPAEILHGDMVQPLRVKVDREARRLAGYRYGRQIADDFLTQ
                     LGQGEEQVARWLEAENDPRLNEIVSHLNHVVEEARIR"
     gene            complement(26448..26990)
                     /gene="hycF"
                     /locus_tag="RH057_000451"
     CDS             complement(26448..26990)
                     /gene="hycF"
                     /locus_tag="RH057_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycF"
                     /protein_id="HDV3126868.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAVDKNFRGKPEQNPQQCIGCA
                     ACVNACPSNALTVEADLATGELAWEFNLGRCIFCGRCEEVCPTAAIKLSQEYELAVWK
                     KEDFLQQSRFVLCSCRVCNRPFAVQKEIDYAIALLKHNGDSRAENHRESFETCPECKR
                     QKCLVPSDRIELTRHMKEAI"
     gene            complement(27000..28709)
                     /gene="hycE"
                     /locus_tag="RH057_000452"
     CDS             complement(27000..28709)
                     /gene="hycE"
                     /locus_tag="RH057_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HDV3126869.1"
                     /translation="MSEEKLGQHYLAALNEAFPGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHSHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGHARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSDAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(28727..29650)
                     /gene="hycD"
                     /locus_tag="RH057_000453"
     CDS             complement(28727..29650)
                     /gene="hycD"
                     /locus_tag="RH057_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HDV3126870.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIFKLLGRQSVGPDASGWVFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTAGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(29653..31479)
                     /gene="hycC"
                     /locus_tag="RH057_000454"
     CDS             complement(29653..31479)
                     /gene="hycC"
                     /locus_tag="RH057_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HDV3126871.1"
                     /translation="MSAISLINSGVAWFVAAAVLAFLFSFQKALSGWIAGIGGAVGSL
                     YTAAAGFTVLTGAVGVSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHA
                     QVKCNGLQINMLMAAAVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALGRL
                     GTLLLAIACWLLWQRYGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWV
                     PQAHANASAPAAALFSTVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGL
                     YALMEHNIQRLLAYHTLENIGIILLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSL
                     FKSVLFLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEW
                     VIYQSFFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAEN
                     ATCAPLLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLI
                     ACPLLPFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKL
                     RKWLNPVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(31476..32087)
                     /gene="hycB"
                     /locus_tag="RH057_000455"
     CDS             complement(31476..32087)
                     /gene="hycB"
                     /locus_tag="RH057_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HDV3126872.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARISTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(32212..32673)
                     /gene="hycA"
                     /locus_tag="RH057_000456"
     CDS             complement(32212..32673)
                     /gene="hycA"
                     /locus_tag="RH057_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HDV3126873.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            32885..33235
                     /gene="hypA"
                     /locus_tag="RH057_000457"
     CDS             32885..33235
                     /gene="hypA"
                     /locus_tag="RH057_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV3126874.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            33239..34111
                     /gene="hypB"
                     /locus_tag="RH057_000458"
     CDS             33239..34111
                     /gene="hypB"
                     /locus_tag="RH057_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HDV3126875.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPKMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHAPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            34102..34374
                     /gene="hypC"
                     /locus_tag="RH057_000459"
     CDS             34102..34374
                     /gene="hypC"
                     /locus_tag="RH057_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HDV3126876.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            34374..35495
                     /gene="hypD"
                     /locus_tag="RH057_000460"
     CDS             34374..35495
                     /gene="hypD"
                     /locus_tag="RH057_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HDV3126877.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            35492..36502
                     /gene="hypE"
                     /locus_tag="RH057_000461"
     CDS             35492..36502
                     /gene="hypE"
                     /locus_tag="RH057_000461"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HDV3126878.1"
                     /translation="MNNIQLAHGSGGQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAVSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGTTILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISEAALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            36576..38654
                     /gene="flhA"
                     /locus_tag="RH057_000462"
     CDS             36576..38654
                     /gene="flhA"
                     /locus_tag="RH057_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HDV3126879.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAEGHIFGGCEFIRYDDRPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     HERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGTGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGASAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     VLPEPETPPAATVVAQEGEDEYQLIVRVLKETNGVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(38691..39044)
                     /locus_tag="RH057_000463"
     CDS             complement(38691..39044)
                     /locus_tag="RH057_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HDV3126880.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKTRE"
     gene            complement(39121..39255)
                     /locus_tag="RH057_000464"
     CDS             complement(39121..39255)
                     /locus_tag="RH057_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3126881.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIASVE
                     "
     gene            39331..41892
                     /gene="mutS"
                     /locus_tag="RH057_000465"
     CDS             39331..41892
                     /gene="mutS"
                     /locus_tag="RH057_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HDV3126882.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRTNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            41998..42654
                     /gene="pphB"
                     /locus_tag="RH057_000466"
     CDS             41998..42654
                     /gene="pphB"
                     /locus_tag="RH057_000466"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV3126883.1"
                     /translation="MPSTRYQKINAHHYRHIWVVGDIHGEYQLLQSRLHQLSFFPETD
                     LLISVGDNIDRGPESLDVLRLLNQPWFISVKGNHEAMALDAFATGDGNMWLASGGDWF
                     FDLNDSEQQEAIDLLLKFHHLPHIIEITNDIIKYVIAHADYPGSEYLFGKEIAESELL
                     WPVDRVQKSLNGELQQINGADYFIFGHMMFDNIQTFANQIYIDTGSPKSGRLSFYKIR
                     "
     gene            complement(42705..43472)
                     /locus_tag="RH057_000467"
     CDS             complement(42705..43472)
                     /locus_tag="RH057_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3126884.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRMNVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVQPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LRSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            43668..44576
                     /locus_tag="RH057_000468"
     CDS             43668..44576
                     /locus_tag="RH057_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV3126885.1"
                     /translation="MKTGSEFHVGIVGLGSMGMGAALSCVRAGLSTWGADLNSNACAT
                     LKEAGACGVSDNAATFAEKLDALLVLVVNAAQVKQVLFGETGVAQHLKPGTAVMVSST
                     IASADAQEIATALAGFDLEMLDAPVSGGAVKAANGEMTVMASGSDIAFERLAPVLEAV
                     AGKVYRIGAEPGLGSTVKIIHQLLAGVHIAAGAEAMALAARAGIPLDVMYDVVTNAAG
                     NSWMFENRMRHVVDGDYTPHSAVDIFVKDLGLVADTAKALHFPLPLASTALNMFTSAS
                     NAGYGKEDDSAVIKIFSGITLPGAKS"
     gene            44573..45835
                     /locus_tag="RH057_000469"
     CDS             44573..45835
                     /locus_tag="RH057_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxo-tetronate kinase"
                     /protein_id="HDV3126886.1"
                     /translation="MIKIGVIADDFTGATDIASFLVENGLPTVQINGVPTGKMPEAID
                     ALVISLKTRSCPVVEATQQSLAALSWLQQQGCKQIYFKYCSTFDSTAKGNIGPVTDAL
                     MDALDTPFTVFSPALPVNGRTVYQGYLFVMNQLLAESGMRHHPVNPMTDSYLPRLVEA
                     QSTGRCGVVSAHVFEQGVDAVRQELARLQQEGYRYAVLDALTEHHLEIQGEALRDAPL
                     VTGGSGLAIGLARQWAQENGNQAREAGHPLAGRGVVLSGSCSQMTNRQVAHYRQIAPA
                     REVDVARCLSTETLAAYAHELAEWVLGQESVLAPLVFATASTDALAAIQQQYGAQKAS
                     QAVETLFSQLAARLAAEGVTRFIVAGGETSGVVTQSLGIKGFHIGPTISPGVPWVNAL
                     DKPVSLALKSGNFGDEAFFSRAQREFLS"
     gene            45832..46470
                     /locus_tag="RH057_000470"
     CDS             45832..46470
                     /locus_tag="RH057_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV3126887.1"
                     /translation="MSDFAKVEQSLREEMTRIASSFFQRGYATGSAGNLSLLLPDGNL
                     LATPTGSCLGNLDPQRLSKVAADGEWLSGDKPSKEVLFHLALYRNNPRCKAVVHLHST
                     WSTALSCLQGLDSSNVIRPFTPYVVMRMGNVPLVPYYRPGDKRIAQDLAELAADNQAF
                     LLANHGPVVCGESLQEAANNMEELEETAKLIFILGDRPIRYLTAGEIAELRS"
     gene            46475..47251
                     /locus_tag="RH057_000471"
     CDS             46475..47251
                     /locus_tag="RH057_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HDV3126888.1"
                     /translation="MPRFAANLSMMFTEVPFIERFAAARKAGFDAVEFLFPYDYSTLQ
                     IQKQLEQNHLTLALFNTAPGDINAGEWGLSALPGREHEAHADIDLALEYALALNCEQV
                     HVMAGVVPAGEDAERYRAVFIDNLRYAADRFALHGKRILVEALSPDVKPHYLFSSQYQ
                     ALAIVEEVARDNVFIQLDTFHAQKVDGNLTHLIRDYAGKYAHVQIAGLPDRHEPDDGE
                     INYPWLFRLFDEVGYQGWIGCEYKPRGLTEEGLGWFDAWR"
     gene            47340..48704
                     /locus_tag="RH057_000472"
     CDS             47340..48704
                     /locus_tag="RH057_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntP family transporter"
                     /protein_id="HDV3126889.1"
                     /translation="MSTITLLCIALAGVIMLLLLVIKAKVQPFVALLLVSLLVALAAG
                     IPAGEVGKVMIAGMGGVLGSVTIIIGLGAMLGRMIEHSGGAESLANYFSRKLGDKRTI
                     AALTLAAFFLGIPVFFDVGFIILAPIIYGFAKVAKISPLKFGLPVAGIMLTVHVAVPP
                     HPGPVAAAGLLHADIGWLTIIGIAISIPVGIVGYFAAKIINTRQYAMSVEVLEQMQLA
                     PASEEGATKLSDKINPPGVALVTSLIVIPIAIIMAGTVSATLMPPSHPLLGTLQLIGS
                     PMVALMIALVLAFWLLALRRGWSLQHTSDIMGSALPTAAVVILVTGAGGVFGKVLVES
                     GVGKALANMLQMIDLPLLPAAFIISLALRASQGSATVAILTTGGLLSEAVMGLNPIQC
                     VLVTLAACFGGLGASHINDSGFWIVTKYLGLSVADGLKTWTVLTTILGFTGFLITWCV
                     WAVI"
     gene            complement(48743..48979)
                     /locus_tag="RH057_000473"
     CDS             complement(48743..48979)
                     /locus_tag="RH057_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3126890.1"
                     /translation="MICPRCADEQIEVMAKSPVKDVWTVYQCQHCLYTWRDTEPLRRT
                     SREHYPEAFRMTQKDIDDAPMVPSIPPLLVEGKR"
     gene            complement(48990..50417)
                     /locus_tag="RH057_000474"
                     /pseudo
     CDS             complement(48990..50417)
                     /locus_tag="RH057_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(50417..51010)
                     /locus_tag="RH057_000475"
     CDS             complement(50417..51010)
                     /locus_tag="RH057_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HDV3126891.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            51157..51564
                     /locus_tag="RH057_000476"
     CDS             51157..51564
                     /locus_tag="RH057_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001208089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV3126892.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDPADKRRRFVWLTAEGEKILAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMNA"
     gene            complement(51685..52677)
                     /gene="rpoS"
                     /locus_tag="RH057_000477"
     CDS             complement(51685..52677)
                     /gene="rpoS"
                     /locus_tag="RH057_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HDV3126893.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRE"
     gene            complement(52740..53879)
                     /gene="nlpD"
                     /locus_tag="RH057_000478"
     CDS             complement(52740..53879)
                     /gene="nlpD"
                     /locus_tag="RH057_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HDV3126894.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPAATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            complement(54019..54645)
                     /gene="pcm"
                     /locus_tag="RH057_000479"
     CDS             complement(54019..54645)
                     /gene="pcm"
                     /locus_tag="RH057_000479"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HDV3126895.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            complement(54639..55400)
                     /gene="surE"
                     /locus_tag="RH057_000480"
     CDS             complement(54639..55400)
                     /gene="surE"
                     /locus_tag="RH057_000480"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HDV3126896.1"
                     /translation="MRILLSNDDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     PDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            complement(55381..56430)
                     /gene="truD"
                     /locus_tag="RH057_000481"
     CDS             complement(55381..56430)
                     /gene="truD"
                     /locus_tag="RH057_000481"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HDV3126897.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHAREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEK
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            complement(56427..56906)
                     /gene="ispF"
                     /locus_tag="RH057_000482"
     CDS             complement(56427..56906)
                     /gene="ispF"
                     /locus_tag="RH057_000482"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HDV3126898.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            complement(56906..57616)
                     /gene="ispD"
                     /locus_tag="RH057_000483"
     CDS             complement(56906..57616)
                     /gene="ispD"
                     /locus_tag="RH057_000483"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HDV3126899.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVKRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDAQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            complement(57635..57946)
                     /gene="ftsB"
                     /locus_tag="RH057_000484"
     CDS             complement(57635..57946)
                     /gene="ftsB"
                     /locus_tag="RH057_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HDV3126900.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAAQQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            complement(58140..58463)
                     /locus_tag="RH057_000485"
     CDS             complement(58140..58463)
                     /locus_tag="RH057_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HDV3126901.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFIYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            complement(58513..59118)
                     /gene="cysC"
                     /locus_tag="RH057_000486"
     CDS             complement(58513..59118)
                     /gene="cysC"
                     /locus_tag="RH057_000486"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HDV3126902.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLGVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            complement(59118..60545)
                     /gene="cysN"
                     /locus_tag="RH057_000487"
     CDS             complement(59118..60545)
                     /gene="cysN"
                     /locus_tag="RH057_000487"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HDV3126903.1"
                     /translation="MNTALAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGPTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGTLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEAFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAAPSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            complement(60547..61455)
                     /gene="cysD"
                     /locus_tag="RH057_000488"
     CDS             complement(60547..61455)
                     /gene="cysD"
                     /locus_tag="RH057_000488"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HDV3126904.1"
                     /translation="MDQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            61707..62744
                     /gene="iap"
                     /locus_tag="RH057_000489"
     CDS             61707..62744
                     /gene="iap"
                     /locus_tag="RH057_000489"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HDV3126905.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKSWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGMDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGCCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTAAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
BASE COUNT        14764 a        17250 c        16381 g        14585 t
ORIGIN      
        1 cgctactaaa gccatatcaa gtaagagatg gtgcatccgg gaggattact cgccttgcgg
       61 ctcgcccttc gggccgttgc taaagcaacg ttatcctccc tggtgcttgc ggttagtacc
      121 gccacctctt cgataccttc atcgctgaag attacggaga atatggtgca tccgggagga
      181 ttcgaacctc cgaccgctcg gttcgtagcc gagtactcta tccagctgag ctacggatgc
      241 atcgggaaac ttactttact gcagattttt tgataccgct actaaagccg tatcaagtaa
      301 gagatggtgc atccgggagg attactcgcc ttgcggctcg cccttcgggc cgttgctaaa
      361 gcaacgttat cctccctggt gcctgcggtt ataccgccac ctcttccata ccttcatcgc
      421 tgaatgtacg tagaatatgg tgcatccggg aggattcgaa cctccgaccg ctcggttcgt
      481 agccgagtac tctatccagc tgagctacgg atgcaaatgg cggtgaggcg gggattcgaa
      541 ccccggatgc agcttttgac cgcatactcc cttagcaggg gagcgccttc agcctctcgg
      601 ccacctcacc acacgcctct tacgagtgct tcgaagaact tgtttatcgc tcatcgtcgc
      661 tgcgtggcgc acatattact ttctgagact tataagtcaa acaatttttc ctacactttt
      721 atcgtttgca cacttcacgt tcaattagtc tgtaaaaacg tcaaaaagag tgttttatca
      781 acagaaaaat ggaggtctga cagatagtag taatgcaaaa aaatggagac ttaagttgaa
      841 tgaacgggag taaagcgaaa agactataga gtgaaggaga aattttgagg gtgcgtctca
      901 ccgataaaga tgagacgcgg aaagattagt aactggactg ctgggatttt tcagcctgga
      961 tacgctggta gatctcttca cggtgaacag aaacttcctt cggggcattt acgccaatac
     1021 gtacctggtt gccctttacc cctaaaactg tcacggtgac ctcatcccca atcatgaggg
     1081 tctcaccaac tcgacgagtc agaatcagca ttctttgctc cttgaaagat taaaagagtc
     1141 gggtctctct gtatcccggc attatccatc atataacgcc aaaaagtaag caatgacaaa
     1201 cacattacat gtaagcagtc atggcattac attctgttaa acctaagttt agccgatata
     1261 cacaacttca acctgacttt atcgttgtcg atagcgttaa tgcgaatgcc gtgaagcgag
     1321 tccacggcat tgcctgacgc ttatattatt gcaatttcgc gctgacccag cctttcacac
     1381 tggctaacgc tgcaggtaag gccgcagcat ccgtaccacc ggcttgcgcc atgtcaggac
     1441 gtccaccacc cttgccgccc acctgctgag cgaccatacc aatcagttcc cctgctttca
     1501 cacgatctgt gacgtcctta gatacgcctg caatcagaga aaccttacct tcggctaccg
     1561 ttgccagcac gataattgtc gaccccagct gattttttaa atcgtcaacc atggtacgca
     1621 acattttcgg ctcaacaccg ctaagctcgc taaccaacag cttaacacca ttaacatcaa
     1681 ttgccttact ggaaagattt gcgctctcct gtgcggcagc ttgttcttta agctgttgta
     1741 gttctttttc taactgacgc gtacgttcca gtactgagcg cactttatcc gccagattat
     1801 tgctatcgcc tttcagcaga tgcgcgactt cgcttaagcg atcgctgtct gcatgaacgg
     1861 tggtgatagc gccttctccg gttaccgctt cgatacgacg aacgcctgca gcagtacccg
     1921 attcagagat gatgcggaac agaccaatat caccagtgcg gctggcgtga gtaccgccac
     1981 acagctcggt ggagaaatcg cccatgctca gcacgcgtac acgctcatca tacttctcgc
     2041 cgaacagcgc catcgcacct ttcgctttcg ccgcttcgag atccatgatg ttggtttcga
     2101 tcggcaaatt gcgacgaatc tgtgcgttca ccaggtcttc gaccgcacga atctcttccg
     2161 gtttcatcgc ttcgttgtgt gagaagtcga agcgcagcac cttgtcgtta accagtgaac
     2221 ctttctgcga tacatgagtc cccagaacct ggcgcagcgc agcgtgcatc aggtgcgttg
     2281 cggagtgatt cagacgaata cgggcgcgac gagcctcatc aacatcagcc tgcacagcgt
     2341 cgcccacttt cagagaaccc gcagcaagtt taccgatgtg accaatcgcc tggccgtatt
     2401 tctgcgtatc ttccaccgcg aaggagaagt tagcgccttt cagttcgcct ttatcaccaa
     2461 cctgaccgcc ggattccgca tagaatggtg tttgatccag cacgaccaca gcttcctggc
     2521 ctgcattgat ggcatcaacc gctttaccat caacaaacag cgcggtcact ttgccgttca
     2581 gttccagatg gtcatagcct ttaaattcag atgcactatc aacacggatc attgcgttgt
     2641 aatcggcacc aaagccgctg gcttcgcgcg cgcgacgacg ctgctcttcc attgctgctt
     2701 caaaaccagc ttcgtcaact ttgatgttgc gctcacgaca aacatcagcc gtcaggtcaa
     2761 ccgggaagcc ataggtgtcg tacagacgga aagccgtttc accatccagc gtatcaccag
     2821 aaagttttgc cagctcttca tccagcaacg ccagaccgcg ctccagagta cgggcaaact
     2881 gctcttcttc agtcttcagc acctgctcaa cctgcgcctg ctggcgtttc aggtcttcac
     2941 ccgcagagcc cataacgtcg atcagcggac caaccagttt gtagaagaag gtttctttcg
     3001 cgccgagcat attgccgtga cgcactgcgc gacgaatgat acgacgcagt acataaccac
     3061 ggttttcatt ggacggcatt acgccatccg cgatcaggaa cgcacaagaa cgaatgtggt
     3121 cagcgattac gcgcagcgat ttattgctca gatcggtcgc gccagtgact ttcgctaccg
     3181 cctggatcaa cgtgcggaac aggtcgatgt catagttaga gttaacgtgt tgcagcaccg
     3241 cagcaatacg ctccagaccc ataccggtat ctacagacgg cttcggcagc ggttccatcg
     3301 tgccatctgc ctggcggttg aactgcatga agacgatgtt ccagatctca atgtagcggt
     3361 cgccgtcttc ttccggactt cccggagggc caccccaaat gtggtcgccg tgatcgtaga
     3421 agatttcggt gcacgggccg cacggaccag tgtcacccat ctgccagaag ttgtcagatg
     3481 cgtatggcgc acctttgtta tcgccgatgc gaataatacg ttcgcgcggg atcccgactt
     3541 ctttttccca gatttcgtag gcttcgtcgt cgctttcata gacggtaacc cacagacgct
     3601 ctttcggcag ggcaaaccat ttttcgctgg tcagcagttc ccatgcaaac tgaatggcat
     3661 cgtgtttgaa atagtcgccg aagctgaagt tgcccagcat ttcgaagaag gtatggtgac
     3721 gcgcggtgta accgacgttt tccaggtcgt tgtgtttacc acccgcacgc acgcagcgtt
     3781 gggaagtggt agcgcgggaa taattacgct tgtcgagccc aaggaacaca tccttgaact
     3841 ggttcatccc ggcgttggta aacaacaaag ttgggtcgtt atggggtacc agggagctgc
     3901 tggcaactac ctgatgtccc ttactatgga aaaagtcgag aaacgcctga cggatctcag
     3961 cggtgctctt gctcataatt atcctgaaat caagctaacg aaatatcgcc accagctcca
     4021 gcgtgtctta accgccgggc tggtaactga aaagtgggaa taagataagt tttcttgact
     4081 gggaagtaaa ataccgtatg cgttcagtcg gcaaaatttc gccaaatatc ctggatatct
     4141 tccatcagat agccacgata gagcagaaaa cgctggatct taactttttc tgaaaagaca
     4201 gttggcaaag gttcaccata ttttcgcgtc gcctgatcgc gcgccagtgc gcaccagtcg
     4261 atgtcacatt cacgcatcgc tttttctgtc gcttcgcggg aaataccttt ctgattcagt
     4321 tcctggcgaa tacgcgcagg tccataacct ttgcggctac ggctggcgat aaagcgcgca
     4381 acaaatcggc tgtcatcgag atagccatgt tcatggcacc aggcaataac gcgctcgtaa
     4441 tcttctgccg tagcgtcaat ctcttctggg ccatttttgc ccataatcgg tgccgcgagt
     4501 ttacgtcgca gttcttgctc actgtgatcg cgcaccgcca gaatgcgtac cgcacgatcc
     4561 aacaggcgag catatgccgg gcgacgggac gttgattctg tcatggcata tccttacaac
     4621 ttaaaaaagc aaaagggccg cagatgcgac ccaggttttg ctgataaaca gatttaatag
     4681 tcaatgagtg tgcagaatgt tcccgcaggc ccaattcgtt agaggatcga ggtgagagta
     4741 atttttcacg ccagtgctgg cctgttcccc tcaccctaac cctctcccca aaggagcgag
     4801 gggaccgtcc gtgcacacat tatctattat catcaataac gaacgattaa aaatcttcgt
     4861 tagtttctgc tacgccttcg ctgtcatcta cagagaaatc cggcgttgag ttcgggttgc
     4921 tcagcagcaa ctcacgtact ttcttctcga tctctttcgc ggtttccggg ttatctttca
     4981 gccaggcagt tgcattcgct ttaccctgac cgatcttctc acctttgtag ctgtaccacg
     5041 cgcctgcttt ctcgatcagc ttctctttta cgcccaggtc aaccagttcg ccgtagaagt
     5101 tgataccttc gccgtagagg atctggaatt cagcctgttt aaacggcgca gcgattttgt
     5161 tcttcaccac tttcacgcgg gtttcgctac ccaccacgtt ttcgccctct ttcaccgcgc
     5221 cgatacgacg gatgtcgaga cgaacagagg cgtagaattt cagcgcgtta ccaccggtag
     5281 tggtttccgg gttaccgaac atcacaccaa ttttcatacg gatctggttg atgaagatca
     5341 gcagcgtgtt ggactgcttc aggttacccg ccagcttacg catcgcctgg ctcatcatac
     5401 gtgccgcaag gcccatgtga gagtcgccga tttcgccttc gatttccgct ttcggcgtca
     5461 gtgccgccac ggagtcaacg acgataacgt ctactgcgcc agaacgcgcc agggcgtcac
     5521 agatttccag tgcctgctcg ccggtgtccg gctgggagca cagcaggtta tcgatatcga
     5581 cgcccagttt acgtgcgtag attgggtcca gcgcgtgttc agcatcgata aacgcacagg
     5641 ttttaccttc acgctgcgct gcggcgatca cctgcagcgt cagcgtggtt ttaccggaag
     5701 attccggtcc gtagatttcg acgatacggc ccatcggcag accacctgcc ccaagcgcga
     5761 tatccagtga aagcgaaccg gtagagatgg tttccacatc catggaacgg tcttcaccca
     5821 ggcgcatgat ggagccttta ccaaattgtt tctcaatctg gcccagtgct gccgccaacg
     5881 ctttctgttt gttttcgtcg atagccattt ttactcctgt catgccgggt aataccggat
     5941 agtcaatatg ttctgttgaa gcaattatac tgtatgctca tacagtatca agtgttttgt
     6001 agaaattgtt gccacaaggt ctgcaatgca tacgcagtag cctgacgacg caccgcatca
     6061 cggtcgccgc tgaagcattc ccgccgggta atgccttcac cgcgggcagt ggcaaaagca
     6121 aaccagacgg tgccgacagg cttctcttca ctgccgccat ccggcccggc gataccacta
     6181 atagacacgg cataatcagc acgagccgct ttcagtgcgc ctatcgccat ttccaccacg
     6241 acgggttcac tcaccgcgcc atgctgcgcc agcgtctctt cgcgtacgcc gatcatctgc
     6301 gctttggctt cgttactgta ggtgacaaat ccgcgttcaa accaggcgga gctaccggca
     6361 atatcggtaa tcactttcgc tacccaacca ccggtacaag actcggcagt tgttacggtt
     6421 gcgccacggg ctttcagcgc ctgcccaacc tgttcactta actgcatcag ttcactgtca
     6481 gtcatcacaa cctctgtcag tcaaaaattt atgccggaca agatagcact ttcgtcagga
     6541 agatggtgtt ggcgttgcaa ttttacgagg aggattcaga aaaaagctga ttagccagag
     6601 ggaagctcac gcccccctct tgtaaatagc tactgtactc gcgccagcgc cacggcttgt
     6661 cctaactgcc agaccgccat tgcgtaatgg gtgctgtggt tgtaacgggt gatggtgtag
     6721 aagttcggca gaccgtacca gtactggtag ccggtgccaa catccagacg cagcaggctg
     6781 gcttgttgat ggttgcccag cggctgctgt ggcgttaaac ctgcggcggc aagctgcgaa
     6841 atgctgtact tagttttgaa gccatttggc aagcctggag cctgaccgtt tgccattacc
     6901 gcgacctgat cgcctttcac ccagccgtgc gctttgaaat agttcgccac gctaccgatc
     6961 gcatcaaccg gatcccacag gttgatatgc ccgtcgccgc tgaaatctac cgcatattgt
     7021 ttgtaagacg acggcataaa ctgtccgtag cccatcgccc cggcaaagga acctttcaga
     7081 ttgagcggat cgtcctgctc gtcgcgcgcc atcaacagga aggtttccag ctcgccagag
     7141 aaatactccg cgcggcgtgg gtagttaaat gacagcgttg ccagcgcatc gaggatgcga
     7201 gttttcccca tcacgcgccc ccagcgggtt tcaacgccga taatcccgac gataatttcc
     7261 ggcggtacgc catacacctg ccacgcgcga ttcaacgcat cttcatactg attccagaaa
     7321 accacaccgt tctgcacgtt gtccggcgta ataaattttt tgcgataacg cagccatgcg
     7381 ccgttcggac ctgatggcgg tttcaccgat gtggttggtg cctggttatc catcagccgc
     7441 agtaccgaat ccagacgttt cgcctgggag agaatttcct gcaactgctg acgatcgaaa
     7501 ccgtgtttgt tcaccatttt gtcgatgaac tgctgggcat tcgggttatt agcgaaatcg
     7561 ccgcccatct gcatcacatt gtgctgcggc tcaagcagga agccgccaga cggcgttccg
     7621 gtggtcgtat cagtctcagt aggttttggc ttgctgctac aggcggcaag caacacaaaa
     7681 aggggaagca atgttacata acgacgcttg aacatgaggg gtccatttaa cagattcaac
     7741 caggggcaag tatggtaaag catcacgccc cgcacaagga agcggtagtc actgcccgat
     7801 acggacttta cataactcaa ctcattcccc tcgctatcct tttattcaaa ctttcaaatt
     7861 aaaatattta tctttcattt tgcgatcaaa ataacacttt taaatctttc aatctgatta
     7921 gattaggttg ccgtttggta ataaaacaat aaatcctgaa ggagagaaca atgatagaaa
     7981 ccattactca tggtgcagag tggtttatcg ggctgttcca aaagggcgga gaggtgttta
     8041 ccgggatggt gaccggcatt cttccgctgt tgattagcct gctggttatc atgaacgcac
     8101 tgattaattt tatcggtcag catcgtattg aacgttttgc tcaacgttgc gccggtaacc
     8161 ctgtttcccg ttacctactg ttaccgtgca ttggcacgtt tgtcttttgc aatccgatga
     8221 ccctaagcct gggtcgcttt atgccggaaa agtacaaacc cagctactac gcggcggcct
     8281 cttatagctg ccactcaatg aatggcctct tcccccatat caaccctggc gaactgtttg
     8341 tttatcttgg cattgccagc ggtctgacaa cgctgaacct gccacttggc ccactggcgg
     8401 tgagttatct gctggttggt ctggtcacca atttcttccg cggctgggtg accgatctga
     8461 ccaccgccat ttttgagaaa aagatgggca ttcaacttga acaaaaagtt cacctggcag
     8521 gagcaacatc atgacgcata ttcggatcga aaaaggaacg ggtggctggg gcggcccgct
     8581 tgagctgaaa gccacgccgg gaaaaaaaat cgtctatatc accgccggta cccggcctgc
     8641 gattgttgac aaactggcac agcttactgg ctggcaggct attgacggat ttaaagaagg
     8701 tgaacccgcg gaggcggaaa ttggtgtcgc ggtaatcgac tgtggcggca cattacgctg
     8761 cggcatctat ccgaaacgac gtattcccac cattaatatc cactcgacgg gcaagtccgg
     8821 tccgctggcg cagtacattg tggaagatat ttatgtctct ggcgtaaaag aagaaaacat
     8881 cactgtagtg ggtgatgcga caccacaacc ctcttccgtg ggccgtgact atgacaccag
     8941 taagaaaatc accgaacaaa gcgatggttt actggcgaag gtgggaatgg gcatggggtc
     9001 cgccgttgcg gtgctgtttc aatctggtcg tgacaccatc gacactgtat taaaaaccat
     9061 tctgccgttt atggcattcg tctcggcgct cattggcatc attatggctt ctggccttgg
     9121 tgactggatt gcccacggtc ttgctccgct ggcgagccat ccactgggtc tggtcatgct
     9181 ggcgctcatc tgctccttcc cactgctttc acctttcctc ggcccaggcg cagttatcgc
     9241 acaggttatc ggcgtattga ttggcgtgca gattggtctc ggcaatattc cgccgcatct
     9301 ggctttacct gcactgtttg ccatcaacgc gcaggcggcc tgcgacttca tcccggtcgg
     9361 tttgtcgctg gcggaagctc gtcaggacac ggttcgcgtc ggtgtccctt ctgtactggt
     9421 gagccgcttt ttaaccggcg cgccgactgt actgatcgcc tggtttgtct ccggttttat
     9481 ctatcaatag aggctgaaac atgaccgtta tttatcagac caccatcacc cgtatcggcg
     9541 cgagtgccac tgacgccctc agcgaccaga tgctcatcac ctttcgtgaa ggcgcgccag
     9601 cggacctcga agagtattgc ttcattcatt gccacggcga gttgaaaggt gcactccatc
     9661 ccggtttgca attttcactc gggcagcatc gctatccggt gaccgctgtt ggcagcgtgg
     9721 cggaagacaa ccttcgcgaa ctgggtcatg tcaccctgcg cttcgatggt ttaaacgaag
     9781 cggaatttcc gggcactgtc catgtggcag gccctgtacc cgacgatatc gcgccgggat
     9841 cggttttgaa gtttgaatct gttaaggagt aaaaaatgaa tcaggttgcc gttgtcatcg
     9901 gtggtgggca aacattaggc gcgttcctgt gccacggtct ggctgccgag gggtatcgcg
     9961 tcgcggttgt cgatattcag agcgacaaag ccgcaaatgt ggcacaagaa attaacgccg
    10021 aatatggtga aagtatggcg tacggttttg gtgctgacgc cactagcgag caaagcgttc
    10081 tggcgctctc tcgtggggta gatgaaatct ttggtcgcgt ggatttgctg gtctacagcg
    10141 ccggaatagc aaaagcagcc tttatcagcg acttccagct cggcgatttt gaccgttcgc
    10201 tacaagtgaa tctggtgggt tatttcctgt gtgcccgtga attttcgcgt ttgatgatcc
    10261 gcgacgggat tcagggtcgc attattcaga tcaactcgaa atccggcaaa gtgggcagca
    10321 aacacaactc tggctacagc gcagcgaaat ttggtggcgt cgggctgact caatcactgg
    10381 cgctggatct ggcggagtac ggcattacgg tgcattcact gatgctcggt aacctgctga
    10441 aatcgccgat gttccagtca ctgttgccac aatacgcgac caagctgggt atcaaaccgg
    10501 atcaagtcga gcagtattac atcgacaaag taccgctcaa acgcggctgc gattatcagg
    10561 atgtgctgaa tatgctgctg ttctatgcca gtcctaaggc gtcgtactgc accggacagt
    10621 cgatcaatgt caccggcggt caggtgatgt tctgatcaac agcggagatc cgttaaggat
    10681 ctccgtgaga ctaatagaat gcctgatgcg ctacgctcat caggcctaca ggacttccgc
    10741 cactacatta aggaaaagtt atggtatccg cactcatcac cgtcgccgtt atcgcctggt
    10801 gtgcgcaact ggccttaggc ggctggcaaa tttctcgttt taaccgtgcc ttcgacacac
    10861 tatgccagca aggtcgggtt ggcgtgggcc gttccagcgg gcgctttaaa ccgcgggtcg
    10921 tggtcgccat cgcgctggac gatcagcagc gtatcgtcga taccttgttt atgaaaggac
    10981 tgaccgtctt cgcacgaccg caaaaaattc ccgcaattac cggcatgcat gcgggtgatt
    11041 tacagcccga tgtgatcttt ccccatgatc cactatcaca gaatgctcta tcattggcgc
    11101 ttaaactgaa acgtggataa tttcgttgtg aatgttactt gcttgcgaag ttatcatttt
    11161 gaaacctaaa tcaggtaatc acgcccatga aacctcgtca gcgtcaggcc gccattctgg
    11221 agtatctgca aaagcagggt aaatgctcgg ttgaagaatt ggcgcaatac tttgacacca
    11281 caggcacaac cattcgcaaa gatctggtca ttctggaaca tgccggaacc gtcattcgta
    11341 cttatggcgg agtggtgttg aataaagagg aatccgatcc gcctatcgat cataaaacac
    11401 tcatcaacac ccacaagaaa gagctgattg cagaagctgc cgttagtttt atccatgatg
    11461 gcgattcgat cattcttgat gctggcagta ccgttttgca gatggttccc ctgctctcgc
    11521 gctttaataa catcacggtg atgaccaaca gcctgcatat cgtcaatgcg ctatccgaac
    11581 tggataacga acaaactatc ctgatgccag gcggaacgtt tcgtaaaaaa tcggcctcat
    11641 ttcacgggca gctggcagag aatgccttcg agcatttcac cttcgataaa ttgtttatgg
    11701 gcaccgacgg catcgatctc aatgcgggcg taaccacgtt caacgaggtt tataccgtca
    11761 gtaaggcaat gtgcaatgcc gcgcgcgaag tgattttgat ggcggactca tcaaagtttg
    11821 gccgtaaaag ccccaacgta gtttgcagtc ttgaaagcgt cgataagctg attaccgacg
    11881 caggtatcga tccggcgttt cgtcaggcgc tggaagagaa agggatcgat gtgatcataa
    11941 ccggagagag caatgagtga agcactactg aacgcgggac gtcagacgtt aatgctggag
    12001 ttgcaggaag caagccgatt accggaacgt ctgggcgatg actttgttcg cgccgccaat
    12061 atcatcctgc actgcgaagg caaagtggtg gtttcgggaa ttggcaaatc gggccacatt
    12121 ggtaagaaaa tcgccgcaac acttgccagt accggcacac cggctttttt tgtccatccg
    12181 gcagaggcac tgcacggcga tctggggatg attgaaagcc gcgatgtgat gctgtttatc
    12241 tcttactccg gtggcgcgaa ggaactggat ctgattattc cgcgtctgga agataaatct
    12301 aacgcgctgc tggcgatgac cggcaaaccg acgtcaccgc tgggcctggc ggcaaaagcg
    12361 gtgcttgata tctccgtaga acgcgaagcc tgcccgatgc accttgcgcc gacctccagc
    12421 accgtcaata ccctgatgat gggtgacgcg ctggcgatgg cggtcatgca ggcgcgcgga
    12481 tttaatgaag aagattttgc ccgctcccac ccagccgggg cactgggcgc tcgcttgctg
    12541 aataaagtgc atcatctgat gcgccgtgac gatgccatcc cacaggtggc gttaaccgcc
    12601 agcgtgatgg atgcgatgct ggaactcagc cgcaccggtc tggggctggt ggcggtatgt
    12661 gacgctcaac aacaggtaca aggcgtcttt accgacggcg atttacgtcg ctggctggtt
    12721 ggcggcggcg cactcaccac gccagttaat gaagcgatga cggtcggcgg caccacgttg
    12781 caatcgcaaa gtcgcgccat cgacgccaaa gagatcctga tgaagcgcaa aatcactgcc
    12841 gcaccggtgg tagatgaaaa cggcaaactc accggcgcaa ttaacctgca ggatttctat
    12901 caggccggga ttatttaatc cttcattccc agacgtttcg ccagccgatg caggttggcg
    12961 acgtcggttt ccagcatccg cgcgcaggca gcccagttgt gatgattttg tgccagcgcc
    13021 tgacgaatag tttcacgctg gaacgcttct gtcgcttcac gcaggttttg cttaacaacg
    13081 ggcaccgccg ccgcttctgg cggcggcaac gtcacctcag gaaaagcaaa atgttgcgcc
    13141 tcaagaatca cttcatcgcc gttgcgggtg gctctcgaca gcactaccgc ccgatgaata
    13201 gcatgttcca gttcgcgcac gttccccgga aaacgatagt gttgcagtaa atttcgcgct
    13261 ccggcactta ataccacgcg ggagagcccc agccgcaaac gacactgctc gcagaaatac
    13321 cccgccagca gaatgacatc atcgccccgc tcacgcagcg gcggcaccga aagtggaaac
    13381 acgctcaggc gatgaaacaa gtcagcgcga aatcgccctg ccagcacctc ttcgcgtaag
    13441 tcgcggttag tcgccgccag cacgcgcaca tcgacccgca aactgcggtc atcgccaacg
    13501 cgctgaatat cgccatactg caacaccctc agcagcttgg cctgcaatgc caacgacaac
    13561 tcgccgatct catccagaaa cagagtgccg ttatccgcca tttcaaactt cccgctgcgg
    13621 ttactgatag cgccagtaaa cgctcctttc acatgcccga acagttcact ttccgccaca
    13681 ctttccggca gtgcggcaca gttgagatag accagcggat tcaccgcccg tggcgaggct
    13741 tcatgaatcg ctttcgccac cagctcctta ccggttcccg tctcaccgct gatcaggacg
    13801 ttgagatcgg acgccgccac aatctcaatc tcttttttca gttgcgtcat gccaggggac
    13861 aagccaatca tctgcgtctg tttcaccgct tcaaacggcg tggcatcgcc tggcatcata
    13921 ttctggcttt ccagttgttc aatcagcaac gcattgctta acgctcccgc cgccagcgca
    13981 gcaatcagcc gtagctcttc gtcgctgaaa acatcgaact gatcgggctg catcccgtcg
    14041 agcgtcagtg cgccgatcag gttttgcccg gcaaacaatg gcagaccaac gcaggcgtga
    14101 accttcagac tctcctgccc aggaatcaaa ccgtcatagg gatcgggcaa ttcgctgtct
    14161 gcgggaaagc gcaccacatc cccggcgcgg gcaatcgctt ccagccgtgg atgcccttcc
    14221 agcgcaaagc gtctaccgag tacatccttt gccagaccgt cgatggcaag cggaataaac
    14281 tgccgcgaat cgtaacgtag caacgcagac gcatcgcact ccagcacctg acgtagcgtg
    14341 gtgatcaggc gctgaaaacg atcctgatga ccaatcccac gctgcaattc gatggcgata
    14401 ttcgccagca catcaacgga aaaactcatc tttgcctcac tgtcaatttg actatagata
    14461 ttgtcatatc gaccatttga ttgatagtca ttttgactac tcattaatga gcatactttt
    14521 atttatatag taaaaacaat cagataaaaa actggcacgc aatctgcaat tagcaagaca
    14581 tctttttaga acacgctgaa taaattgagg ttgctatgtc tattgtggtg aaaaataaca
    14641 ttcattgggt tggtcaacgt gactgggaag tgcgtgattt tcacggcacg gaatataaaa
    14701 cgctgcgcgg cagcagttac aacagctacc tcatccgcga agaaaaaaac gtgctgatcg
    14761 acaccgtcga ccataaattc agccgcgaat ttgtgcagaa cctgcgtaat gaaatcgatc
    14821 tggcggatat cgattacatc gtgattaacc atgcagaaga ggaccacgct ggggcgctga
    14881 ccgaactgat ggcgcaaatt cccgatacgc cgatctactg tactgccaac gctatcgact
    14941 cgataaatgg tcatcaccat catccggagt ggaattttaa tgtggtgaaa actggcgaca
    15001 cgctggatat cggcaacggc aaacagctca tttttgtcga aacaccaatg ctgcactggc
    15061 cggacagcat gatgacttac ctgacaggcg acgcggtgct gttcagtaac gatgctttcg
    15121 gtcagcacta ctgcgacgag catctgttca acgatgaagt ggatcagacg gagcttttcg
    15181 agcagtgcca gcgttactac gccaatatcc tgacgccgtt cagccgcctg gtaacgccga
    15241 aaattaccga gatcctgggc tttaacttac cagtcgatat gatagcgacc tcccacggcg
    15301 tggtctggcg cgataacccg acgcaaattg tcgagctgta cctgaagtgg gccgctgatt
    15361 atcaggaaga cagaatcacc attttctacg acaccatgtc gaataacacc cgcatgatgg
    15421 ctgacgctat cgcccaggga attgcggaaa ccgacccacg cgtggcggtg aaaattttca
    15481 acgtcgcccg aagcgataaa aacgaaatcc tgaccaatgt cttccgctca aaaggcgtgc
    15541 tggtcggcac ttcgacgatg aataacgtga tgatgccgaa aatcgccggg ctggtggagg
    15601 agatgactgg tttacgcttc cgtaacaaac gcgccagtgc tttcggctct cacggctgga
    15661 gcggcggtgc ggtggatcgt ctttccacgc gcctgcagga tgcgggtttc gaaatgtcgc
    15721 ttagcctgaa agccaaatgg cgaccagacc aggacgctct ggagttatgt cgtgaacacg
    15781 gtcgcgaaat cgcccgtcag tgggcgctcg cgccgctgcc gcagagcacg gtgaatacgg
    15841 tggttaaaga agaaacctct gccaccacga cggctgacct cggcccacgg atgcagtgca
    15901 gcgtctgcca gtggatttac gatccggcaa aaggcgagcc aatgcaggac gttgcgccag
    15961 gaacgccgtg gagtgaagtc ccggataact tcctctgccc ggaatgctcc ctcggcaaag
    16021 acgtctttga agaactggca tcggaggcaa aatgagtaac ggcattgtga tcatcggttc
    16081 gggcttcgcc gcccgccaac tggtgaaaaa tattcgcaaa caggacgcca ctattccatt
    16141 aaccctgatt gccgccgaca gcatggatga gtacaacaaa cctgacctca gccatgttat
    16201 cagtcagggg caacgtgccg atgaccttac ccgccagacg gcgggtgaat ttgccgagca
    16261 gtttaatctg cacctgtttc cacaaacctg ggtgacggat atcgatgccg aagcccgtgt
    16321 ggtgaaaagc cagaataatc agtggcaata cgacaagcta gtactggcaa ccggtgccag
    16381 tgcctttgtc ccgcctgtgc ctgggcgtga gttaatgctg acgttaaata gtcagcaaga
    16441 gtatcgcgcc tgtgaaacgc aactgcggga tgcccgacgc gtgttgattg ttggcggtgg
    16501 tttgattggt agcgaactgg cgatggattt ttgtcgtgca ggcaaagcgg tcacgctaat
    16561 cgacaacgct gccagtattc tggcgtcatt aatgccaccg gaagtaagca gccgcttgca
    16621 gcatcggttg acggagatgg gcgttcatct gttgttgaaa tctcagttgc aggggctgga
    16681 aaaaacagat tctggcattc tggcaacgct ggaccgccag cgcagtatcg aagtggatgc
    16741 ggtaattgcc gccaccggac tgcgcccgga aaccgccctg gcacgacgcg ccgggctgac
    16801 gattaatcgc ggcgtttgcg tcgatagtta tctgcaaacc agtaatgccg atatttacgc
    16861 gctgggcgat tgcgcggaaa ttaacggtca ggtattgccg ttcctccagc cgattcaact
    16921 tagcgcgatg gtgctggcaa aaaatcttct cggcaataac acgccactga aactaccggc
    16981 gatgctggtg aaaatcaaaa cgccggaatt accgctgcat ctggcaggcg aaacccagcg
    17041 tcaggattta cgctggcaaa ttaataccga acgccaggga atggtggcgc gcggcgttga
    17101 cgatgctgac cagcttcgcg cctttgtggt cagtgaggat cggatgaaag aggcatttgg
    17161 attgttgaaa acattgccga tgtaggtggg ctactgtgcc taaaatgtcg gatgcgacgc
    17221 tggcgcgtct tatccaacct acggggagcg catgtgttgg tcacatactg cgttcatgtc
    17281 gcatccacct actgtgccca aaatgtcgga tgcgacgctg gcgcgtctta tccgacctac
    17341 ggggacgcat gtgtaggccg gataaggcgt ttacgccgca tccggcaatg gtgtccaaat
    17401 gcaacacgtt ttatccgttc tggacttcac ccgctaacca acgcgccgca gcaataaccc
    17461 cctgccccag agacaaaccg ccatcacccg ccggtaaact ctgtggaaag agcaatgtga
    17521 aatcagcgag ataatgcgcc agacgtgcac gcagcaaacg gttatgaata accccgccgc
    17581 taaataccag cgtagtgata ccacgcatcg tggcctgctc acgcatcaac gcggcaaaac
    17641 cctgcgccag cgcatcatga aacgcccacg cgcgttgatt aaccggtgcc tgccagttca
    17701 gccactgctg ccagaaagtg gcgagatcca gttgattgtc caccagcggc attgtcaccg
    17761 gatgcgtcac tccgtggcac gaagctgcga gtgcctccag agcacaagcc gcttcacctt
    17821 cataacttaa cgtggctggc gcacagccca gtgccgccgc cacggcatcg aacaaccgcc
    17881 cacacgatga cgccagcggc gcgttaattc cacgctcaat ggcccgcgcc agcacgctcc
    17941 agttttgctg ttgcacactt gctgtttcag aataattctg ccactccggc acaaagcgca
    18001 ggcactgcgc cagcaggttt cgccacggct gcttcgccgc caaatcgcca cccggaagcg
    18061 ccactgcagg caagccgccc aggtgctcac attcgcgata gttcacccgc agacactcgc
    18121 cgccccacaa agcgccgttc tcccccatac cgataccatc gagcgtcaaa gcaatgacat
    18181 caccgccatc cagcggccac tgatgctctg ccagacacgc cgctgcgtgg gcatgatgat
    18241 gcagcaccgt ttgcgtcggc agattcattt cgcgcgccca ctggctggag acatagcccg
    18301 gatgtgcgtc atgcacaacg tattgcgggg tgaaatcgta gatgttttgc atcaggcgta
    18361 acgcttcgcg ccactgcatc tggatgccat cgtcacttaa atcgcccaga tgctgactca
    18421 acactgcttg ttcaccgcgc accaggcaga aggtgttttt cagatctgcg ccgagacaca
    18481 gcacaggcgg cacattttta aagcccggag gcaaagccag tgcgtccggt acataccccc
    18541 gcgaacggcg cagcatttcg ccgctttcgc gcaccaccga atcatccatc cgctgcacga
    18601 tgtcgcggtt atgtatcaag aatccgtcgg caatgccctg caaatccgcc agcgcctgtt
    18661 cgttgctgat agccggtggt ttaccgctca ggttgccgga ggtcatcacc agcgggcatt
    18721 gcagttcctg taacagcaaa tgctggagcg ggttcgcagg caacatcacg ccgacttcgt
    18781 taaggccagg ggcgatatca tcacaaagct caggaacgta ttttttatcc accagcacaa
    18841 tcggcgcggc gggcgtggta agcaactggc gcgcagcgtc tggtaaacct tctgccactg
    18901 gcaagatgac cgccagcggt ttcgccgggc gatgtttgcg cgcccgaagt gtcgccaccg
    18961 cggtactgtt acgtgcatcg caggcaagat gaaatccgcc aatccctttg atggcgacaa
    19021 tgttgcccat ttttaactgt gcgatagccg cctgtaatgc cgcctcttgt tccgcatgtt
    19081 caccatgact tacccattca agatgtgggc cacactccgg gcaggccacc ggctgggcgt
    19141 ggaagcgacg atcgagcggg tcgcggtact ctttatcaca ggctggacac agcggaaact
    19201 ccgccattac ggtaaacggg cggtcgtaag gcatggcgcg aataatggtg aaacgcgggc
    19261 cgcagtgggt acagttgata aacggataac gataacgccg ttcgcctggg gtattcattt
    19321 cggcaaggca agcagggcaa gtagcggcat ccgggacaat ttgcgtattc atggcacctc
    19381 ccgcgctctg gcggatagtg aactcggtgg gcagttgtga ccagataaac ggctcacgct
    19441 cgacgctatc aatacgcgcc agtggcggac agtgctgatg caattgaaca agaaacgttt
    19501 ccgggtcttc cagcagccgg acttctacgc catcgccgtc attacagaca tcgccgtgaa
    19561 gatttaattg ctgtgccagc tgccagacaa acggacgaaa accgacgccc tgcactttgc
    19621 cacgaatacg cagttggaca ccgcaagatg tgttttttgc cattgagtta ttcccgccat
    19681 catgaattgc gtaacccgtc cagccggaca cgacagcgtc gcatccggca gtcacaggtc
    19741 ggcgataccg cccgcttcgt attctacgaa tatttccggg aattcctttg atgccagaac
    19801 agttctgtaa gatttttaga acatcagcgc cgtacggcgg cgtttttctg cgctcagttg
    19861 ttcaagttta ttacgatcga cacaaattag cgcatgggtc gggcaagccg ccatacacgc
    19921 cgggccgtct tcacgatggt tgcacaggtc gcatttattg gcttcagctt tgtcagcccg
    19981 tacattcagc cccgcgccgc tgttgcggat caccggacgt accaccactt ccatcgcgcc
    20041 atacgggcaa gccaccacgc aggttttgca accaatgcaa cgttcctgca tcacatgaac
    20101 aaacccttta tcacggctga tagcaccatt cgggcagacg ttagcgcacg gtgcatcttc
    20161 acactgacgg cagactgtcg ccgtggaaat gttcacacct ttaatgacat ggattcgcgg
    20221 taaaaaggtt tccggggtca gcgatgcaca gtcctgattt tcctgatgag aaaccacgca
    20281 cgccacttca caggtacggc aaccaataca tttactcgcg tcagcaatga tgaaacggtt
    20341 catcaaattc tccagcaatg acagttaatg cgccgataca ttcacaaatc atgccagttt
    20401 ttaatttgct gttatttaag gaaattaatt tctgtaatgc aggaaaaaag atgtcatcga
    20461 cactagtgac gatgacatgt gatgacaatg tttatcgcga aagggcaatg agtgagtcgc
    20521 ggcggatcag ttttccgctg aaggttttcg gcggtgagaa atccccgcca tcgagcataa
    20581 aaatcagccg tccaataatt tcctgaatca tctctgttac cggaattttt acgctggaga
    20641 gcgccggaac ggtgtagggg gcaattgcga tatcatcgaa tccgataact gacacctgct
    20701 ctggcaccgc tacgccgcgc tcgtgcaacg ctttcatcgc acctatcgcc atatcgtcgt
    20761 tactggcaac taacgcgcta aatttagccc cacgttcgag caacgtttct accccttcgg
    20821 ccccgctggc gggcgtccat ttaccgttag cgataagttt ttcattgagc gcaataccat
    20881 gctgcgccag cgcgtcttta tacccagcaa gacgttcaat gctggtgggg gaatccatcg
    20941 agccggtaag gaaagcaatc tcctgatgtc cggcgtttat caactctgcc acggcattaa
    21001 agctggtctg tttatgatcg caccagacgc tatggctgct gtttttgcgc aggcggcgat
    21061 taagcaccat tatcggctga ctgtgcgcgt caatgatgtc atcaatctca tccacgctta
    21121 aaaaacgcgg gtaaatcata atggcgtcgc agcgcaaatc cagcagatac tgaatcgcct
    21181 ggcgctcttc ttcggcgctg tgtttaccat ctgccaatag caactgccgc cctttctctt
    21241 ccgccattcg cgcggcatga aagagtaatt cactaaaata aatgccgtgg taaagcgtgt
    21301 tggtcactac cagccccagc gtctgagtac tcttcgccga cagattgcgc gccagcaagt
    21361 ttgggcggta accgctctct tctaccgcct gaaacacgcg atctttagtc tcctggctga
    21421 cgtagccatt acctgaaagc acgcgggaaa cggtcgcttt tgaaaccccg gcgcgcttcg
    21481 ccacttccag catcgtcgtc atcattttca tccctttaca cgcaatcaac gcagtgtact
    21541 gcaccgtttg ccgattgtcc ttgcgcaatc ggcgggaaaa atattcaggt gaccggtttc
    21601 acaaatataa aaaatgaaca attaactctc ttgcttattt agtgacaact attcatgatt
    21661 ttgtgaaacc ggtttcctaa ttccgtttca gcatcggcat ttttccgtta cgtcgactga
    21721 taacaactac atctacccta ctgataacag gataaaatcc gatggccaaa aattatgcgg
    21781 cgctggcacg ctcggtgata gcggcactgg gcggcgttga taacatctcg gcagtcacgc
    21841 actgtatgac gcgtttgcgc tttgttatca aagatgacca gcttatcgac agcccgacgt
    21901 taaaaaccat ccccggcgtg ctcggcgtgg tacgtagtga caaccagtgt caggtgatta
    21961 tcggcaatac cgtttcacaa gcctttcagg aagtcgtcag cctgctgccg ggagatatgc
    22021 agcccgcaca gcccgtgggt aaacccaaac tcacgctgcg tcgcattggt gcggggatcc
    22081 ttgatgcgct gatcggcacc atgtcaccgc tgatcccggc gattatcggc ggatcgatgg
    22141 tcaaactgct ggcgatgatc ctcgagatga gcggcgtgct gacaaaagga tcgccgacct
    22201 taaccattct gaatgtgatt ggtgacggtg ctttcttctt cctgccgcta atggtcgcgg
    22261 catctgccgc catcaaattt aaaaccaata tgtcgctggc gattgccatc gcaggtgtgc
    22321 tggtacatcc gagctttatt gaactgatgg cgaaagcggc ccagggtgaa catgtcgaat
    22381 ttgccctgat tccggtcacc gcggtgaaat atacctacac ggtgatcccg gcgctggtga
    22441 tgacctggtg cctgtcatat atcgaacgct gggtggatcg cattacaccg gctgtgacca
    22501 aaaacttcct caagccaatg ctgattgtgt tgattgccgc accgctggca atcctgctga
    22561 ttggcccgat tggtatctgg atcggtagcg ccatttcggc gctggtttac accattcatg
    22621 gttatctggg ctggctttca gtcgccatta tgggcgcgct gtggcctctg ctggtaatga
    22681 ccgggatgca ccgcgtcttt acgccaacca tcattcagac cattgccgaa accggcaaag
    22741 aagggatggt catgccgtca gagatcggcg ctaacctgtc gctgggcggt tcatcactgg
    22801 cggtggcgtg gaaaacgaaa aacccggaac tgcgccagac ggcgctggct gcggcggcat
    22861 cagccattat ggcggggatt tccgaaccgg cgttatacgg cgtggcgatc cgcctgaaac
    22921 gtccgcttat cgccagtctt atcagcggtt ttatttgcgg cgcggttgcc ggtatggcgg
    22981 ggcttgccag ccactcaatg gcagcgccgg ggttatttac cagcgtgcaa ttcttcgatc
    23041 cggcgaatcc aatgagcatc gtctgggtgt tcgcggtcat ggcgctggcg gtggtgctgt
    23101 cgtttatcct cacactattg ctcggctttg aggatattcc tgttgaggaa gcggctgccg
    23161 aggcgcgaaa gcatcagagc gcacaaccga ccgtcgccaa agaagtaagt cttaattgag
    23221 gatgaaaatg tcagtatttc cagaaggttt tttatggggc ggcgcgcttg ccgccaacca
    23281 gtctgaaggt gcgttccgtg aaggtggcaa aggactgacc acggtcgata tgatcccgca
    23341 cggcgagcat cgaatggcgg tgaaactggg gctggaaaaa cgttttcagt tgcgcgatga
    23401 cgagttttat cccagccatg aggcgacgga tttttatcat cgttataaag aagatatcgc
    23461 cctgatggca gagatgggat tcaaggtttt ccgtacctca attgcctgga gccgcctctt
    23521 tccgcagggc gatgaaatca cgcccaatca gcagggcatt gctttttacc gttctgtctt
    23581 tgaagagtgt aaaaagtacg gtatcgaacc gctggtcacg ttgtgccact tcgatgtgcc
    23641 gatgcatctg gtcaccgaat atggctcctg gcgtaaccgc aagctggtgg agtttttcag
    23701 ccgctacgcc agaacctgct ttgaagcatt tgatggtctg gtgaaatact ggctaacctt
    23761 caatgaaatc aacattatgt tgcatagccc gttctccggc gcgggtctgg tgtttgaaga
    23821 aggtgaaaat caggatcagg tgaaatatca ggccgcgcat caccagctgg ttgccagtgc
    23881 gctagccacc aaaatcgccc atgaggttaa cccgcaaaat caggtgggct gtatgctggc
    23941 gggcggtaac ttctaccctt acagctgcaa accggaagat gtctgggcgg cgctggagaa
    24001 agatcgggaa aacctgtttt ttatcgatgt gcaggcgcgc ggcgcgtatc cggcttactc
    24061 tgcccgcgta tttcgcgaaa aaggggtaac catcaacaaa gcaccgggcg atgatgaaat
    24121 cctgaaaaac accgtcgatt ttgtctcttt cagctattac gcctcgcgct gcgcctcggc
    24181 ggagatgaac gccaacaaca gcagtgcggc gaacgtggtg aaatcgctgc gtaatccgta
    24241 tctacaggtg agcgactggg gctggggaat tgatccactc ggtctgcgta tcaccatgaa
    24301 tatgatgtac gatcgttatc agaagccgct gtttctggtg gaaaacggcc tcggcgcaaa
    24361 agatgaactt gctgccaatg gcgagattaa cgatgactat cgcatcagct atttacgcga
    24421 acatatccgc gcaatgggcg aagcgattgc agatggcatt ccgctgatgg gctacaccac
    24481 ctggggctgt attgatttag tttccgcctc tacgggtgaa atgagcaaac gctacggttt
    24541 tgtctacgta gaccgtgacg acgcaggcaa cggcacgctg acgcgcacgc gtaagaaatc
    24601 gttctggtgg tataaaaaag taattgccag taatggggaa gatttagagt aagtaaccgt
    24661 gccggatgcg gcgtgaacgc cttatccggc ctacatgtcg cacgaaccat gaaggcccga
    24721 taagcgcagc gcatcgggca atttagcatt tggcatcaga gctggttcac cccatcaaga
    24781 acatccctgt cctgattcct taatgaaaaa ctactctact tccaccgcta actgcgcaaa
    24841 tccaccattt ccttcccagc cttccagtcg ttgataaacg gtttcaaccg catctttaat
    24901 cggctgggtc atcgggtagt aaaagccgac gatatccggc tggatgccga ggaaaatcac
    24961 ttcgccaata tcttctttca actggtcgat aaggtagttc aacggcatgt tatgggtagt
    25021 catcataaac atctcggcga tatcatccgg gtcgatgatg cggatctcgc cggggtttag
    25081 ccccatatca gtggcgtcga caatcagcag tcgcgtaggg cgcagttcac ggatggcgac
    25141 gatgtcgttt tccggtgcac taccgccgtc aatcaccacc cagttacctt tcggcgcggc
    25201 ggcgcacttt tccgccagca gcgggcctgc gccatcatcg cccatcatgc tattgccaac
    25261 acagagtaaa acgtcagtca cgcagtctcc tcaccatcaa atagatggcg ttttcctgat
    25321 gaatatcatg cagcatactt agcagcattg tactccattc ctgctgctgc gaagtttgcg
    25381 ctgcccgggc tttatcgaat gcgttggcca gcatcggcac atggttaatg tcgatgacga
    25441 tctcaccata tttcggcacg ccttccattt tgcggcgggc ttcgctgccc gcctccagag
    25501 tggcaatcca tgccagatat tcatcccacg ggcaggtgag cgccgcttcc aggcaatcga
    25561 taaccccaag gtggtgacca atcgccaggc tgtaatagac cacctgctgc gcctcggcgg
    25621 gcgtggcatc gttctcatca ataaatttac ggctcagttg actgaacacc accttttcac
    25681 tcatcggata cgcgcctctt ctacaacatg attcagatgg ctgacaatct cgttcagacg
    25741 cggatcgttt tccgcttcca gccagcgtgc aacctgttct tcgccctgcc ctaactgtgt
    25801 caggaagtca tcggcaatct gacgaccgta acgataacct gccaggcggc gtgcttcgcg
    25861 atccactttc acacgcagcg gctgcaccat atcgccatgc aggatttccg ccggttgttc
    25921 atcctgctca cccgggccac gggcgtgaat tttctgctcc agcaggccga gcgccattgc
    25981 aaagccgtac agcgtggcgg caggcgttgg cgggcaacca gggatgtaga catctaccgg
    26041 gacgatttta tccgtaccgc cccacacgca gtagagatcg tggaagatcc cgccactgtt
    26101 accgcaggca ccgtaggaga tacagatttt cgggtccggc gcggactgcc acgcacgcag
    26161 cgcaggggat cgcattgcac gggtgaccgc gccggtaaac agtaaaatat ccgcatgacg
    26221 cggtgaagga acgactttaa tgccgaagcg ttctgcatca aacagcggcg aaagcgtggc
    26281 gaaaatttcg atttcgcagc cgttgcagcc gccgcagtcc acgcggtaaa cgtaggcaga
    26341 acgtttgatt tttttcagta acgacgcctt catgctggcg atggattcat ccaccgtcat
    26401 cgggaccgga atgccgttgg cgtcacgggg gcctaataaa ttgctcatca gatggcctct
    26461 ttcatatggc gagtcagttc aatacggtcg gacggcacca ggcatttctg gcgcttacat
    26521 tccgggcaag tctcaaagct ttcgcggtgg ttttccgcgc ggctgtcacc attgtgctta
    26581 agcagcgcaa tggcgtagtc gatctctttc tggacggcga aaggacgatt gcagacgcga
    26641 cagctgcaca gcacaaaacg ggactgttgc aggaaatctt ctttcttcca caccgccagt
    26701 tcgtactctt gcgacagttt gatcgctgcc gtcgggcaga cttcttcgca gcgtccacag
    26761 aagatgcagc gcccgagatt aaactcccac gcaagctctc ctgtggcgag gtcagcttca
    26821 accgttaagg cgtttgacgg gcaggcattg acgcaggccg cgcagccgat gcactgctgc
    26881 gggttctgct ctggcttacc acggaagttt ttatcaaccg caatcggctc cagcggataa
    26941 gacgaggtcg ccgtgccggt tttgatgact tttttgataa aggtaaacat ggcgattcct
    27001 tatttcagcg gcgagttttt acgctcaatg ctgtaacgct cgagttcttt gtacggcacc
    27061 actttgctct tcttcttacg cacatcgacc acggtcatgc ggtcggtaca ggagtagcaa
    27121 gggtccaggc taccgataat cagcggcgca tcggaaacgg tattgccacg cagcatgtag
    27181 cgcagggtcg gccagttcgc gtaggtcgcg gcacggcagc gccagcggta cagcttctgg
    27241 ttgtcgccgg tcatgctcca gtggatatcg tcgccgcgcg gcgcttcggc aaagcccagc
    27301 gcgaaacggt gcggaatgta ggtaaagcct tccaccatca gcggaccacc cggcaagtta
    27361 tccagaccgt agtcgatcat gttcagcgcg gtatagactt cgttgatacg cactttcaga
    27421 cgggaaataa cgtcgcagcc ctgctcgctg tggacttcca ttggcagcag gccataacca
    27481 acaaacgggt gatcggcgcg ggtatcgcga gcgtgtccgc tggcgcggac catcgggcca
    27541 acgttactga aatcgcgggc gatttccggg tccagacgac caatgccgac agtgcgctgt
    27601 tccatgttcg gcgtactcag cagcacatcc accagctcct gcacttcacg acgcatttgt
    27661 tgcgccagct ggcgggtctg gatcatgtca tctttcagca gatcgcgacg aatcccgccg
    27721 atcaggttca ggccgtaggt tttacgcgca ccggtaagga tctctgccat tttcatggag
    27781 gtttcacgca cgcggaagaa ctgcataaag ccggagtcga agccagtaaa gtgacaggcc
    27841 aggccgaggt tgagcagatg cgagtgcagg cgttccacct ccagcagaat ggcgcggatc
    27901 atctgcgcac gttctggcac ctgaataccc atcgcgtttt ccaccgacgt ggtgtaggcg
    27961 gtgctgtgag caaagccgca gatgccgcac acacggtcag agaggaaggt cacttcgttg
    28021 taccccatac gggtttccgc cagtttttcc atgccgcgat ggacgtagaa caggcggtag
    28081 tcggcgtcga taatattttc gccatcgacg aacagacgga agtgacccgg ttcgtcggaa
    28141 gtgacgtgca acggaccaat cggcacgacg ttgtttttct tgtcacccag ttcattgatg
    28201 aactcgtagg tttcagcatc ggtggtcggc gccggacgct gacgataatc catgctgtct
    28261 ttacgcagcg gataaagttc atccggccag tcatccggca gcaccagacg acgttcatcc
    28321 ggcagaccaa ccggaatcaa accgtacata tcgcgcactt cgcgctcgcc ccacacagcc
    28381 gccggaacgc gcggcgtcac ggacggatac tccggtttgt tggcgtcaac ttcgacgcga
    28441 accgttaccc aacacttagt gcccttctcc atcgacagca cgtaataaac ggcgtaatga
    28501 ccattcagtt tgcgttcgtc gttaccaaac agcaccgaca gccagccacc ctgtttgtag
    28561 taaagaaact ccaccacttc cggcaggtag ttcaccttta cggtgacagt cagctgatct
    28621 ttggtctgcc aggcgtggtc cagcacgacg cccggaaatg cctcattcag cgcggcgaga
    28681 taatgttgac ctaatttttc ttcagacatg ctcaaactct ctttaatcac gccgccagca
    28741 aggagacgaa cgctaaaaat gcaaagccaa acccagccca ggtaatgcgc ggagtaatat
    28801 caagacgcag acgggccatg ctgttttcga acagcgcgat aaccaggacg ccgaccacca
    28861 gtttgacgat ggcaatcacc agcgccagca gcagtccacc agcggtgaag gtttccattt
    28921 gtccccacgg aataaacacc ccgacgaaca tctgcaacac caccagctgt ttcaggctga
    28981 taccccattt catgacgcca aagccgctac cgctgtattc ggagagcgga ccttcctgca
    29041 actcctgctc ggcttccgcc aggtcgaacg gcagtttgcc catttcgata aaagtggcga
    29101 acgcacaggc acaaagcgcc agcaccagcg ggatgctctg gctcagcggc cagtgataaa
    29161 cggtgtcggt gatgttgctg atgttggtgg aaccggcaac ctgtgcggca acccacagac
    29221 caagcagcag catcggttcg accagcacgc caagcatcgc ttcacggctc gcgccgatag
    29281 cagtaaacgg gctaccggta tccagaccgg aaatagcaaa gaagaaacgc gcgatggcaa
    29341 agagatacag taaggtgatc aaatcgccca gttgcggcag cggagaaccg acggtcacca
    29401 ccggcagcgc agtagcgata gtcagcatga cgcccaccat cacatacggc gtcaggcgga
    29461 acacccagcc ggaggcatcc gggccaacgc tctggcggcc cagcagtttg aaaatgtcac
    29521 gatactcctg caacacaccc ggcccacgac ggttatgcag acgggcgcgc gccacgcggg
    29581 taataccgga gagcagcggc gcaacggcaa ataacaccag cgcctgaatt aacggatata
    29641 aaacactcat tctcaggctc ctcgtgaaac aataatcacc accagtaccg ccagttcaac
    29701 cagcgccatc cggcggaaca gcaacgcact accttcgcac tgccagcccg gcaccagcga
    29761 caccggattc agccatttgc gcagtttcag cactggcgca aacgcctgtt tcaccggcat
    29821 ggcaaaaccg tgagcggtaa tcaccattga tttttcatgg tcgtagccgc acacccaggc
    29881 cgcaccgcgg gaacgtgacg gcaggcggtc gcctttgcaa atcgccataa tgatgaatgg
    29941 cagcagcggg caggcaatca gcagcaatgt gatcattggt tgagaaacgg tggtgttagc
    30001 aggctccaga ggcagaggta cagcagcaga gagcatcggc agcagccacg gcgcagcaac
    30061 accgccaatt acgcagcaaa tcgccagtgc cactacgctt acgctcatca ggagcggcgc
    30121 acaggtggcg ttttcggctt ctttggtacg cggcgcgccg aggaacgtga cgccatagac
    30181 tttcgccata cacatcaccg ccagcgcacc ggtaattgcc agacccacag cgagcagcgg
    30241 ccccagcaga cgggcaacaa acgcgccgct attgctcagt ttgaagaagg attgatagat
    30301 aacccattcc ccggcaaacc cgttcagcgg cggcagcgca gccattgcca ttaacccgac
    30361 taacatggcg atggagataa ccggcatttt cttgccaata ccaccgagtt tttcgatatc
    30421 gcgatgaccg gtacggaacc agacgctccc cgctccgagg aacagtacgc ttttgaacag
    30481 gctatggtta agcagatggt acagaccacc gaccaggcca agagcaatca gtgccggttg
    30541 ttcgagcgcg atacccgtta cgccagcgcc cagccccagc agaataatgc cgatattttc
    30601 cagggtgtgg taagccagca ggcgctggat gttgtgttcc atcaacgcat acagaccacc
    30661 gacgaacgcg gtgatcatgc cgagcaccag cagcgcgatc ccccaccaca gcggtgcatt
    30721 accgccaagc agtgacaggg ttaacatgcc cagcaggcca attttcatga ctaccgtaga
    30781 aaacaacgcg gcagctggcg cagaggcgtt agcatgtgcc tgcggcaccc agccgtgcag
    30841 cggaataatc ccggccagca ggccaaagcc aatcacaccg agcagccaga tatcggaacc
    30901 gagcggcagc tgttgcatac gcatatccag caggcgcaga tccagcgtgc cataacgctg
    30961 ccacagcagc cagcaagcaa tcgccagcag cagagtgcca agacgcccca gcgcaaacca
    31021 cagtttgccc tctttgctgt tgctggtaag gaacaccgca cacaaggcca tgacttcagc
    31081 cattaccacg aacatgccga ggttgctggc gatgacggcg cagacggcgg cagccatcaa
    31141 catattgatc tgcaagccgt tgcacttcac ctgcgcgtgg cgatgccagt caatgttgta
    31201 gaggctgaca aacagaccgc acagaccgag cgtaatcagc caaatcgcgt taagcggaga
    31261 gatttgcaca tcgtagctta ccagcgacag cgcaccgctc acgccaaccg cgccagtcag
    31321 cacagtaaag cccgcggctg ccgtatacag gctaccaacc gcgccgccaa ttccggctat
    31381 ccagccgctt agcgcctttt gaaaagaaaa gagaaatgcc agaacagcgg cggcgacaaa
    31441 ccacgccacg ccgctattga tcagggaaat tgcgctcatt tagcctctcc actttgagcc
    31501 tgctgaaaca aggtgagatc gccaaagtcc gtgttaaagg tcagctcacg cttacgtttg
    31561 ctaacgcggg cgatatcggt gttatccacc agatgcaggg ctttggtcgg gcacatccgc
    31621 acgcaggccg gaccttgttc atcaaagcta caaaggtcac atttgacggc gatcgcgcga
    31681 atacccggca cccagtcaag cagcgtactg atacgcgccg gagcaggcgg tgccggtggc
    31741 gctttcgggg tattggcatt tgcaggaata tccagcggac ggctgccgga aaattcaatt
    31801 gcgccaaacg ggcaggcgat gccgcacagc ttgcagctta cgcacaggct ttcattcaac
    31861 tgcacggccc catcaacgcg ggtgatggcg ttaaccgggc agaccaccgc gcagggtgca
    31921 tcttcacagt ggtgacagag ctgcggcgca gattcttttt cattcagcat cactctcagg
    31981 cgcggcattg attgcaggcc gtgctggcga tgcgtctctg aacaggcggc ctcacaagtg
    32041 tggcagccga tacagagcgt ggagtcagca attacaaaac gattcaccag gcattcctca
    32101 ggtgattgtc atttttgacg aaaacatgcc gatgaaatgt cattttcgac actcatcgac
    32161 acgcccatcc ccaaacaggc gtaacgcctg caaaacgggc aaagcctcag ctcatgctgc
    32221 cgggctttgt ccctttacca gttggcttaa attcaccggc acattgtttt caatggcggt
    32281 gtataagctg tcgtaaacgc cagcgatttt ttcgagatag tgttccagaa cctgttcgcg
    32341 atcgcggatg ttgaccttgc catcaatcat gccgtcaatg ctcagttgcg cctgcgcaat
    32401 ccgctgtttg tcttcgccat cttttgtttc gacgtaatac tcgatggtgt ggctgttaaa
    32461 gccatccgcc agggtgacgt aaatacgaaa atgttcaaaa aggacgaaac agagttcttt
    32521 gtccggcgcg cagctcatgg cgtgatgcag gcgcgctttc gataacgtga tcccctgaac
    32581 cagcgaattg cagtagatgt gccactggtc ctgtaggcga cgatgccgct gtgcgatgta
    32641 atcggctttc tcgcttattt cccaaatagt cattgtcagg ttacccgttt aacagagatg
    32701 ccagctttaa gcattttttg tgccaacttt taatttattg ttattaaaga gatttttaag
    32761 ataaagatga atttcgtcgc cgtgtcgacg tgtcatttcg acatcatcga cattattcac
    32821 cgcagggata atcaacactg gcacaattat tgcttgtagc tggcaatagt taatgggagg
    32881 cgatatgcac gaaataaccc tctgccaacg ggcactggaa ttgatcgaac agcaggccgc
    32941 aaaacacggc gcaaaacgcg taactggggt ctggctcaaa attggcgcat tttcttgtgt
    33001 cgaaaccagc tctcttgcct tttgttttga tctggtttgc cgcggcagcg tggcggaagg
    33061 ttgtaaactg cacctcgaag aacaagaggc cgaatgctgg tgtgaaacat gccaacagta
    33121 tgtgacgcta ctgacccagc gcgtccgccg ctgtccacag tgtcatggtg acatgctaca
    33181 gattgtggca gacgacggtt tacagattcg gcggatagaa atagaccagg agtgagcgat
    33241 gtgtacaaca tgcggttgcg gtgaaggcaa cctgtatatc gagggtgatg aacataaccc
    33301 tcattccgcg tttcgtagcg cgccatttgc cccggcggca cgcccgaaga tgaaaatcac
    33361 cggcattaaa gcgcctgaat ttacccccag ccagactgaa gaaggcgacc tgcattacgg
    33421 tcatggcgaa gcgggcactc acgcgccggg catgagccag cgtcggatgc tggaagtcga
    33481 aattgacgtg ctggacaaaa ataaccgtct ggctgaacgc aaccgcgcgc gctttgctgc
    33541 ccgcaagcaa ctggtgctca acctggtttc cagccctggt tccggtaaaa ccaccctgct
    33601 gacggaaacc ttaatgcgcc tgaaagacag cgttccgtgc gcagttattg aaggcgatca
    33661 gcaaaccgtg aacgatgccg cacgcattcg cgctaccggc acaccagcga ttcaggtgaa
    33721 cactggtaaa ggctgccacc ttgacgcaca gatgattgcc gacgccgcac cgcgtctgcc
    33781 actggacgat aacggtattc tgtttatcga aaacgttggc aacctcgtat gcccggccag
    33841 cttcgatctc ggtgaaaaac acaaagtagc ggtcctttcc gttaccgaag gtgaagacaa
    33901 accgctgaaa tatccgcata tgtttgccgc cgcctcgctg atgctgctca acaaagttga
    33961 ccttttgccg tatctcaact ttgacgttga gaagtgcatc gcctgcgcac gcgaagtcaa
    34021 tccagaaatt gaaatcatcc ttatttccgc caccagcggc gaagggatgg accagtggct
    34081 gaactggctg gagacacagc gatgtgcata ggcgttcccg gccagatccg caccattgac
    34141 ggcaaccagg cgaaagtcga cgtctgcggc attcagcgcg atgtcgattt aacgttagtc
    34201 ggcagctgcg atgaaaacgg tcagccgcgc gtgggccagt gggtactggt tcacgttggc
    34261 tttgccatga gcgtaattaa tgaagccgaa gcacgcgaca ctctcgacgc cttacaaaac
    34321 atgtttgacg ttgagccgga tgtcggcgcg ctgttgtatg gcgaggaaaa ataatgcgtt
    34381 ttgttgatga atatcgcgcg ccggaacagg tgatgcagtt aattgagcat ctgcgcgaac
    34441 gtgcttcaca tctctcttac accgccgaac gccctctgcg gattatggaa gtgtgtggcg
    34501 gtcataccca cgctatcttt aaattcggcc tcgaccagtt actgccggaa aacgttgagt
    34561 ttatccacgg tccgggttgt ccggtgtgtg tactgccgat gggtagaatc gacacctgcg
    34621 tggagattgc cagccatccg gaagtcatct tctgtacctt tggcgacgcc atgcgcgtac
    34681 cagggaaaca gggatcgctg ttgcaggcaa aagcacgcgg tgccgatgtg cgcatcgttt
    34741 actcgccgat ggatgcgttg aaactggcgc aggagaatcc aacccgtaaa gtcgtattct
    34801 tcggcttagg ttttgaaacc accatgccga ccaccgccat caccctgcaa caggcgaaag
    34861 cccgcgatgt gcagaatttt tacttcttct gccagcatat tacgcttatc ccgacgctgc
    34921 gcagtttgct ggaacagccg gataacggta tcgacgcgtt cctcgcgccg ggccacgtta
    34981 gtatggttat cggcactgat gcctataatt ttatcgccag cgattttcat cgtccgctgg
    35041 tggtcgctgg tttcgaaccc cttgatctac tacaaggcgt ggtcatgctg gtggagcaga
    35101 aaatagcggc ccacagcaag gtagagaatc agtatcgtcg agtggtaccg gatgccggta
    35161 acctgctggc gcaacaggcg attgccgatg tgttctgtgt caacggcgac agcgaatggc
    35221 gcggcttagg cgtgattgaa tcttctggcg tacacctgac gccggattat caacgattcg
    35281 atgccgaagc acatttccgc ccggcaccgc agcaggtctg cgatgacccg cgcgcacgtt
    35341 gtggtgaagt attaacgggc aaatgtaagc cgcatcaatg cccgctgttt ggtaacacct
    35401 gtaatcctca aaccgcattt ggtgcactga tggtctcctc cgaaggagcg tgcgccgcgt
    35461 ggtatcagta tcgtcagcag gagagtgaag cgtgaataat atccaactcg cccacggcag
    35521 cggcggccag gcgatgcagc aattaatcaa cagcctgttt atggaagcct ttgccaaccc
    35581 gtggctggca gagcaggaag atcaggcacg tcttgatctg gcgcagctgg tagcagaagg
    35641 cgatcgtctg gcgttctcca ccgacagtta cgtcattgac ccgctgttct ttcctggcgg
    35701 taatatcggc aagctggcga tttgcggcac tgcgaatgac gttgcggtca gtggcgctat
    35761 tccgcgctat ctctcctgtg gctttatcct cgaagaagga ttgccgatgg agacactgaa
    35821 agccgtagtg accagcatgg cagaaaccgc ccgcacggca ggcattgcca tcgttactgg
    35881 cgatactaaa gtggtgcagc gcggcgcggc agataaactg tttatcaaca ccgctggcat
    35941 gggcgcaatt ccggcgaata ttcactgggg cgcacagacg ctaaccgcag gcgatgtatt
    36001 actggttagc ggcacgctcg gcgaccacgg gacgactatc cttaacctgc gtgagcagct
    36061 ggggctggat ggcgaactgg tcagcgactg cgcggtactg acgccgctta ttcagacgct
    36121 gcgtgacatt cccggcgtga aagcgctgcg tgatgccacc cgtggtggtg tgaacgcggt
    36181 agttcatgag ttcgcggcag cctgcggttg tggtattgaa atttcagaag cggcactgcc
    36241 ggttaaacct gccgtgcgcg gcgtttgcga actgctggga ctggacgccc ttaactttgc
    36301 caacgaaggc aaactggtga tcgccgttga acgcaacgcg gcagagcaag tgctggcagc
    36361 gttacattcc catccactgg gaaaagacgc ggcgctgatt ggtgaagtgg tggaacgtaa
    36421 aggtgttcgt cttgccggtc tgtatggcgt gaaacgaacc ctcgatttac cacacgccga
    36481 accgcttccg cgtatatgct aataaaattc taaatctcct atagttagtc aatgaccttt
    36541 tgcaccgctt tgcggtgctt tcctggaaga acaaaatgtc atatacaccg atgagtgatc
    36601 tcggacagca agggttgttc gacatcactc ggacactatt gcagcagccc gatctggcct
    36661 cgctgtgtga ggctctttcg caactggtaa agcgttctgc gctcgccgac aacgcggcta
    36721 ttgtgttgtg gcaagcgcag actcaacgtg cgtcttatta cgcatcgcgt gaaaaagaca
    36781 cccccattaa atatgaagac gaaactgttc tggcacacgg tccggtacgc agcattttgt
    36841 cgcgccctga tacgctgcat tgcagttacg aagaattttg tgaaacctgg ccgcagctgg
    36901 ccgcaggtgg gctataccca aaatttggtc actattgcct gatgccactg gcggcggaag
    36961 ggcatatttt tggtggctgt gaatttattc gttatgacga tcgcccctgg agcgaaaaag
    37021 agttcaatcg tctgcaaaca tttacgcaga tcgtttctgt cgtcaccgaa caaatccaga
    37081 gccgcgtcgt caacaatgtc gactatgagt tgttatgccg ggagcgcgat aacttccgca
    37141 tcctggttgc cattaccaac gcggtgcttt cccgcctgga tatggacgaa ctggtcagcg
    37201 aagtcgccaa agaaatccat tactatttcg acattgacga tatcagtatc gtcttacgca
    37261 gccaccgtaa aaacaaactc aacatctact ccactcacta tcttgataaa caacatcccg
    37321 cccacgaaca gagcgaagtc gatgaagccg gaaccctcac cgaacgcgtg ttcaaaagta
    37381 aagagatgct gctgatcaat ctccacgagc gggacgattt agccccctat gaacgcatgt
    37441 tgttcgacac ctggggcaac cagattcaaa ccttgtgcct gttaccgctg atgtctggcg
    37501 acaccatgct gggcgtgctg aaactggcgc aatgcgaaga gaaagtgttt accactacca
    37561 atctgaattt gctgcgccag attgccgaac gtgtggcaat cgctgtcgat aacgccctcg
    37621 cctatcagga aatccatcgt ctgaaagaac ggctggttga tgaaaacctc gccctgaccg
    37681 agcagctcaa caatgttgat agtgaatttg gcgagattat tggccgcagc gaagccatgt
    37741 acagcgtgct taaacaagtt gaaatggtgg cgcaaagtga cagtaccgtg ctgatcctcg
    37801 gtgaaactgg cacgggtaaa gagctgattg cccgcgcgat ccataatctc agtgggcgta
    37861 ataatcgccg catggtcaaa atgaactgcg cggcgatgcc tgccggatta ctggaaagcg
    37921 atctgtttgg tcatgagcgt ggggctttta ccggtgccag cgcccagcgc attggtcgtt
    37981 ttgaactggc ggataaaagc tccctgttcc tcgacgaagt gggcgatatg ccactggagt
    38041 tacagccgaa gttgctgcgt gtattacagg aacaggagtt tgaacgtctc ggcagcaaca
    38101 aaatcattca gacggatgtg cgtctaatcg ccgcgactaa ccgcgatctg aaaaaaatgg
    38161 tcgccgaccg tgagttccgt agcgatctct attaccgcct gaacgtattc ccgattcacc
    38221 tgccgccact acgcgagcgt ccggaagata ttccgctgct ggcgaaagcc tttaccttca
    38281 aaattgcccg tcgtctgggg cgcaatatcg acagcattcc tgccgagacg ttgcgcacct
    38341 tgagcaatat ggagtggccg ggtaacgtac gcgaactgga aaacgtcatt gagcgcgcag
    38401 tattgctaac acgcggcaac gtgctgcagc tgtcattgcc agatattgtt ttaccggaac
    38461 ctgaaacgcc gcctgccgca acggttgtcg cccaggaggg cgaagatgaa tatcagttga
    38521 ttgttcgcgt gctgaaagaa accaacggcg tggttgccgg gcctaaaggc gctgcgcaac
    38581 gtctggggct gaaacgcacg accttgctgt cacggatgaa gcggcttgga attgataaat
    38641 cggcattgat ttaactgcaa attgccggac agatctgcct gtccggcata ctattcacga
    38701 gtttttttcg gacgatattt ttccggcagt tctggcaccg gacgcttgtc atcgatgaga
    38761 tgacgcacgg ttaagatcgg atgacgccac agcattcgcg gcccggccca acgcataatc
    38821 tgtttcatct cttcacgctt tgctggctgg taacagtgca ccggacactg cttacaggct
    38881 ggtttctctt cgccgaacac acatttatcc agccgctttt gcgcgtaaac aaacaacgcc
    38941 tcgtaatgct ccggctccgc tgacgcctgc gggcatttcg cttgataaag atcgatcatt
    39001 tttttaatcg tcagtttttc acgagagata cgcttgccgg acatgctgcc tccatactct
    39061 taaggtgtat ttatattaca acttaatttg aaaggggact atggctctga aggaggaggt
    39121 ttattcaacc gacgcaattt tgcgtacttg cttcataagc atcacgcaaa agctgcaaaa
    39181 cagcatcttt cccggaacca gcatcaagaa ctcgccgttc gcttcttccc ctgaaatgat
    39241 taactccggt atcatgtgcg ccttatgtga ttacaacgaa aataaaaacc atcacacccc
    39301 atttaatatc agggaaccgg acataacccc atgagtgcaa tagaaaattt cgacgcccat
    39361 acgcccatga tgcagcagta tctcaagctg aaagcccagc atcccgagat cctgctgttt
    39421 taccggatgg gtgattttta tgaactgttt tatgacgacg caaaacgcgc gtcgcaactg
    39481 ctggatattt cactgaccaa acgcggtgct tcggcgggag agccgatccc gatggcgggg
    39541 attccctacc atgcggtgga aaactacctc gccaaactgg tgaatcaggg cgagtccgtt
    39601 gccatctgcg aacaaattgg cgatccggcg accagcaaag gtccggttga gcgcaaagtt
    39661 gtgcgtatcg ttacgccagg caccatcagc gatgaagccc tgttacagga gcgtcaggac
    39721 aacctgctgg cggctatctg gcaggacagc aaaggtttcg gctacgcgac gctggatatc
    39781 agctccgggc gttttcgcct gagcgaaccg gccgaccgcg aaacgatggc ggcagagctg
    39841 caacgcacta atccggcgga actgctgtat gcagaagatt tcgccgagat gtcgctgatt
    39901 gaaggccgtc gcggcctgcg ccgtcgcccg ctgtgggagt ttgaaatcga caccgcgcgc
    39961 cagcagttga atctgcaatt tggcacccgc gatctggtcg gttttggcgt cgagaacgcg
    40021 ccgcgcggac tttgtgctgc cggttgtctg ttgcagtatg cgaaagatac ccaacgcacg
    40081 accctgccgc atattcgttc tatcaccatg gaacgtgagc aggacagcat cattatggat
    40141 gccgcgacac gtcgtaacct ggaaattact cagaacctgg cgggcggtgc ggaaaatacg
    40201 ctggcttctg tgctcgactg caccgtcacg ccgatgggta gtcgtatgct gaaacgctgg
    40261 ctgcatatgc cagtgcgcga tacccgcgtg ttgcttgagc gccagcaaac tattggcgca
    40321 ttgcaggatt tcaccgccga gttgcagccg gtactgcgtc aggtcggcga cctggaacgt
    40381 attctggcac gtctggcttt acgaactgct cgcccacgcg atctggcccg tatgcgccac
    40441 gctttccagc aactgccgga gctgcgtgcg cagttagaaa ctgtcgatag tgcaccggta
    40501 caggcgctac gtgagaagat gggcgagttt gccgagctgc gcgatctgct ggagcgagca
    40561 atcatcgaca caccgccggt gctggtacgc gacggtggtg ttatcgcatc gggctataac
    40621 gaagagctgg atgagtggcg cgcgctggct gacggcgcga ccgattatct ggagcgtctg
    40681 gaagtccgcg agcgtgaacg taccggcctg gacacgctga aagttggctt taatgcggtg
    40741 cacggctact acattcaaat cagccgtggg caaagccatc tggcacccat caactacatg
    40801 cgtcgccaga cgctgaaaaa cgccgagcgc tacatcattc cagagctaaa agagtacgaa
    40861 gataaagttc tcacctcaaa aggcaaagca ctggcactgg aaaaacagct ttatgaagag
    40921 ctgttcgacc tgctgttgcc gcatctggaa gcgttgcaac agagcgcgag cgcgctggcg
    40981 gaactcgacg tgctggttaa cctggcggaa cgggcctata ccctgaacta cacctgcccg
    41041 accttcattg ataaaccggg cattcgcatt accgaaggtc gccatccggt agttgaacaa
    41101 gtactgaatg agccatttat cgctaacccg ctgaatctgt cgccgcagcg ccgtatgttg
    41161 attattaccg gcccgaacat gggcggtaaa agtacctata tgcgccagac cgcactgatt
    41221 gcgctgatgg cctacatcgg cagctatgta ccggcacaaa aagtcgagat tggacctatc
    41281 gatcgcatct ttacccgcgt aggcgcggca gatgacctgg cttccgggcg ctcaaccttt
    41341 atggtggaga tgactgaaac cgccaatatt ttacataacg ccaccgaata cagtctggtg
    41401 ttaatggatg agatcgggcg tggaacgtcc acctacgatg gtctgtcgct ggcgtgggcg
    41461 tgcgcagaaa atctggcgaa taagattaag gcattgacgc tgtttgccac ccactatttc
    41521 gagctgaccc agctaccgga gaaaatggaa ggcgtcgcca acgtacatct cgatgcgctg
    41581 gagcacggcg acactatcgc ctttatgcac agcgtgcagg acggcgcggc gagcaaaagc
    41641 tacggtctgg cggttgctgc tctggcgggc gtaccgaaag aggttattaa gcgcgcacgg
    41701 caaaaactgc gtgagctgga aagcatttcg ccgaacgccg ccgctacgca agtggatggt
    41761 acgcaaatgt ctttgctgtc cgtaccagaa gaaacttcgc ctgcggtcga ggcactggaa
    41821 aacctggacc cggattcact gactccgcgt caggcgctgg agtggattta tcgcttgaag
    41881 agtctggtgt aataacaact cccgatagtc ttttgctatc gggaatatta acgacaactg
    41941 acgaatcaaa taaaaatacc ctgtataata ggaaagctta ttttacaggg tcaaaccatg
    42001 ccatctacac gctatcaaaa aatcaatgcc catcactatc gccatatatg ggtcgttggt
    42061 gatattcatg gtgaatatca gttattacaa tcccgcttac atcaactctc ttttttcccc
    42121 gaaaccgact tacttatttc ggtaggcgat aacatcgatc gtgggccgga gagtcttgac
    42181 gtcctgcgcc tgctaaacca gccctggttt atctccgtta aaggcaacca cgaagcaatg
    42241 gcgctggatg cgttcgcgac cggcgatggc aatatgtggc ttgccagcgg tggtgactgg
    42301 tttttcgatt taaatgattc agagcaacaa gaggcaatag atctgttgct gaaattccat
    42361 caccttccac atattattga aatcactaac gacataataa aatatgtcat cgcacatgca
    42421 gattatccgg ggagtgaata tctctttggt aaagaaatag cggagagcga attactctgg
    42481 cctgttgatc gtgtgcagaa atcgcttaat ggcgagttac aacaaataaa cggcgctgat
    42541 tattttatat ttggacatat gatgtttgat aacattcaga cgttcgctaa ccagatttat
    42601 attgataccg gatcgccgaa aagtgggcgg ctgtcatttt ataaaataag gtagtctcat
    42661 gcttcttctg tgaagcatga gtaaccagat gttattgcag gccattattc atttttcgct
    42721 accagcaaag agagatcctg cttcgccagc gcgcgactgg cactctccgg caaaccgtcg
    42781 tctgtaataa tctgatcaaa ctcgcttaat ggtaacgcca gccatgtcgc cacctgacca
    42841 tatttcgtcg catcacagac caaaactcgc tggcggctgg cactggcaat cgcccgtttc
    42901 accgtgactt tatcttccgc tggcgtagaa atcccccgca cactccatga cgatgcagaa
    42961 ataaaagcct gatcaatcat caggctgcgc agcatggtcg cagcggcttc cccgacacag
    43021 gaacggtttt cccgacacac tgcaccgcca gtgtgaataa ttgtgcaatt actgttgtcg
    43081 agcaagtagt tcgcaataac gaaatcgttt gtgaccacag tcagtgactc catgtgaatc
    43141 agatgctgtg ctatcgctaa cgtggtcgtt cccgcatcca gatagataca acttcccggc
    43201 tgaacaagac ttgccgccag cttgccaata gccgcttttt gcgtcattgc cagcgcagtt
    43261 tttacctgat gagaaggttc atgcgccacg cgtcccggag actggacgcc tccggacacc
    43321 agcacaacgg ctccctgctg ctccagtttt tgtaaatccc gacgaatggt catatgtgac
    43381 acattcattc tgtccgttag ttcagcaata ctgacaatgc ctttttcagc taccatctca
    43441 aggatgattt ggcgacgctc tacgggtatc aacttttgct ccttcctttg tcctgctgac
    43501 attctacgct atttgcctgc gaaacgtgcg cggcgcaact aacgcttagt tcacataaaa
    43561 taacacacaa tgttaattta tgtgaatcag ctcaccatat cgttatcttc cagcgcttat
    43621 attcacaata tcaaacaaaa tatcacttaa attaacaagg agagcagatg aaaacgggat
    43681 ctgagtttca tgtcggtatc gttggcttag ggtcaatggg aatgggagca gcactgtcat
    43741 gtgtccgcgc aggtctttct acctggggcg cagacctgaa cagcaatgcc tgcgctacgt
    43801 tgaaagaggc aggtgcttgc ggggtttctg ataacgccgc gacgtttgcc gaaaaactgg
    43861 acgctctgct ggtgctggtg gtcaatgcgg cccaggttaa acaggtgctg tttggtgaaa
    43921 caggcgttgc acaacatctg aaacccggta cggcagtaat ggtttcttcc actatcgcta
    43981 gtgctgatgc gcaagaaatt gctaccgctc tggctggatt cgatctggaa atgctggatg
    44041 cgccagtttc tggtggtgca gtaaaagccg ctaacggtga aatgactgtc atggcctccg
    44101 gtagcgatat tgcctttgaa cgactggcac ccgtgctgga agccgttgcc ggaaaagttt
    44161 atcgcatagg tgcagaaccg ggactaggtt cgaccgtaaa aattattcac cagttgttag
    44221 cgggcgtaca tattgctgcc ggagccgaag cgatggcact tgcagcccgt gcggggatcc
    44281 cgctggatgt gatgtatgac gtcgtgacca atgccgccgg aaattcctgg atgttcgaaa
    44341 accggatgcg tcatgtggtg gatggcgatt acaccccgca ttcagccgtc gatatttttg
    44401 ttaaggatct tggtctggtt gccgatacag ccaaagccct gcacttcccg ctgccattgg
    44461 cctcaacagc attgaatatg ttcaccagcg ccagtaacgc aggttacggg aaagaagacg
    44521 atagcgcagt tatcaagatt ttctctggca tcactctacc gggagcgaaa tcatgatcaa
    44581 gattggcgtt atcgccgatg attttaccgg cgcgacggat atcgccagtt ttctggtgga
    44641 aaacggtcta ccaacggtac aaattaacgg tgttccaaca ggtaaaatgc cggaagcaat
    44701 cgacgcactg gtgatcagcc tgaaaacgcg ctcctgtccg gtggttgaag ccacacagca
    44761 atcgctggcg gctctgagct ggttgcaaca gcaaggttgc aaacagatct atttcaaata
    44821 ctgctctact ttcgacagta cggcgaaagg taatatcggc ccggttaccg atgcattaat
    44881 ggatgctctc gacacgccgt ttacggtctt ctctccggcc ctgccggtca acggacgtac
    44941 ggtttatcag gggtatttgt tcgtaatgaa tcaactgctg gccgaatccg ggatgcgcca
    45001 tcacccggta aatcccatga ccgacagcta tcttccccgt ctggttgaag cgcaatccac
    45061 agggcgctgc ggcgtcgttt cggcacatgt tttcgaacaa ggtgtggatg ccgttcgtca
    45121 agagctggct cgcttacagc aagagggcta ccgctacgcg gtgcttgatg cgctgaccga
    45181 acaccatctg gaaattcagg gagaagcctt gcgcgatgcc ccactggtaa cgggcggttc
    45241 tggtctggcg attggcctgg cccggcagtg ggcgcaagaa aacggtaacc aggctcgcga
    45301 agcagggcat ccgctcgctg ggcgcggcgt agtgctctcc ggttcatgct ctcaaatgac
    45361 caaccgccag gtggcacatt accgtcaaat tgcaccagcc cgtgaagttg atgtggcacg
    45421 ctgcctctca accgaaactc tggccgctta tgcacacgaa ctggcagagt gggttctggg
    45481 ccaggaaagt gtacttgctc cactggtttt tgccaccgcc agcactgacg cattggcagc
    45541 aattcaacag caatacggtg cacaaaaagc cagtcaggca gtagaaactc tattttctca
    45601 actagcggcg cggttagcag cggaaggcgt gacacgcttt attgtcgcag gcggtgagac
    45661 ctccggcgta gtcacacaga gcctgggcat aaaagggttt catattggcc caaccatttc
    45721 ccccggcgtg ccgtgggtaa acgcactgga taagcctgtc tcactcgccc ttaaatctgg
    45781 caacttcggt gatgaagcct ttttttcacg agcccaaaga gagtttttat catgagcgat
    45841 ttcgcaaaag tagagcagtc tttgcgagag gagatgacgc ggattgccag ttcattcttt
    45901 cagcgcggct atgcaaccgg ttcggctggc aatctgtcgc tgcttttacc tgacgggaat
    45961 ttactggcga caccgacagg ttcatgcctg ggcaatctcg atccgcagcg gctttccaaa
    46021 gtcgccgcgg atggcgaatg gttaagtggt gacaaaccct cgaaagaggt gctctttcat
    46081 ctggcgctgt atcgcaacaa tccgcgctgt aaagcggtgg tgcatttgca cagcacatgg
    46141 tcgacggcgc tttcctgcct gcaagggctg gacagcagca acgttattcg tccgttcaca
    46201 ccatacgtgg tgatgcggat gggaaatgtc ccgctggtgc cttattaccg accgggcgat
    46261 aaacgcatcg cacaggatct ggcggaactg gcagcagaca atcaggcttt tttactggca
    46321 aatcatggcc cagtggtttg cggtgaaagc ctgcaagaag ccgccaacaa tatggaagag
    46381 ctggaggaaa cggcaaagct gatttttatt ctcggtgacc gcccgatccg ttatctgacc
    46441 gcaggtgaaa ttgcggaatt aaggagttaa tgcaatgcct cgttttgcag ctaatttatc
    46501 catgatgttc accgaagtgc cttttattga acgcttcgcc gcagcgcgaa aagccgggtt
    46561 cgatgctgtg gaatttctgt ttccctatga ctactccacc ctgcaaatcc aaaagcaact
    46621 ggagcaaaac catctgacac tggcgctgtt caataccgct cccggagata ttaatgcggg
    46681 ggaatgggga ttatccgccc ttcccggacg tgagcacgaa gcacacgcag acatcgacct
    46741 ggcactggaa tatgcattgg cgctcaattg tgaacaagtc catgtgatgg caggcgtcgt
    46801 gcccgctggc gaagacgctg aacggtaccg ggcagtattt atcgataatc tccgctacgc
    46861 tgccgaccgc tttgcgctac acggcaagcg aatattagtt gaagcactca gccccgacgt
    46921 taagcctcat tatctcttct ccagccagta tcaggcactg gctattgttg aagaggttgc
    46981 gcgagataac gtgtttattc aactagatac ttttcatgcg caaaaagtag atggcaacct
    47041 gacgcattta atccgcgact acgctgggaa atatgcgcac gtacaaattg ccggactacc
    47101 tgatcgacat gaaccggacg atggagaaat caactatccg tggctgttcc gcctgttcga
    47161 tgaggtggga tatcagggct ggatcggttg tgaatataaa cctcgtggcc tcaccgaaga
    47221 agggcttggc tggtttgacg cctggcgcta attcgttaaa tccctaatta caacgtaccc
    47281 atacatcccc ccatctacgg atggggtagg ttaactataa ctttcagaca gggtttccca
    47341 tgtccacaat tacattgtta tgcattgcgt tagctggcgt aatcatgctg ttgctgctgg
    47401 tcatcaaggc aaaggtacaa ccattcgttg ctctgctcct cgtcagcctg ttagtcgcac
    47461 ttgcggcggg tataccggcg ggcgaagtgg gtaaagtgat gatcgccggg atgggcggtg
    47521 tgcttggttc cgtcactatt attattggtc tgggcgctat gctggggcgt atgatcgaac
    47581 actctggtgg tgcagagtca ctggctaatt atttcagtcg caagttaggt gacaaacgaa
    47641 ctatcgctgc gctgactctg gcagcgttct tcctcggtat tcccgtcttc tttgatgtcg
    47701 gctttattat tcttgcgcca atcatttacg gttttgccaa ggttgccaaa atatcgccac
    47761 tcaaatttgg cctgcctgtc gctggaatca tgctaactgt tcatgtggcg gtaccgccgc
    47821 atccaggccc tgtcgccgca gcggggttac tccacgcaga catcggctgg ctaaccatca
    47881 tcggtattgc gatttctatt cccgtaggga ttgttggcta ctttgcagcg aaaataatca
    47941 atacgcgcca atatgcgatg tcggtggaag tactggaaca gatgcaactg gctccggcca
    48001 gtgaggaagg cgcaacaaaa ttaagcgata aaataaatcc accgggcgtc gcgctggtca
    48061 cctcgctaat tgttattcct atcgcgatta tcatggcggg tacggtttcc gcaacactga
    48121 tgccgccttc gcatcccctg cttggtacgc tacagctgat cggctcacca atggtagctc
    48181 taatgattgc gctggtgctg gcattctggt tattggcttt acgtcgcggc tggagcttac
    48241 aacacaccag cgacattatg ggctcagccc ttcccactgc ggcggtagtg attttggtta
    48301 ctggtgctgg aggggtattt ggcaaagtgc tggtggaatc gggcgtcggc aaagcccttg
    48361 ccaatatgct acaaatgatt gacctgcctc tgttaccagc cgcatttatt atttcactgg
    48421 ctctgcgtgc atcgcagggg tcagcaaccg tagcaatcct gacaaccggc gggttactct
    48481 cagaagcggt gatgggattg aatccgattc agtgcgtatt ggtgacgctg gcagcctgct
    48541 ttggtgggct tggcgcgtca catattaatg actcagggtt ctggattgtg accaaatatc
    48601 tggggttgtc ggtagcagac ggtctgaaaa cctggacagt gttaacgacc attctcggtt
    48661 ttaccggatt cttaattacc tggtgcgtat gggcggtaat ttgaccttaa taaaaaggtc
    48721 cgatgggcat cggacctttt atttaacgct taccttccac cagcagcggc gggatgctcg
    48781 gcaccattgg cgcgtcatca atatctttct gcgtcatgcg gaacgcttcg ggataatgtt
    48841 cgcggctggt acggcgcagc ggttcggtat cgcgccaggt ataaaggcaa tgctggcact
    48901 gatataccgt ccagacatct ttcaccggcg atttcgccat cacttcaatc tgttcatcgg
    48961 cacaacgtgg acaaatcatc ttcttctcct tatttacgtg cagccagcat agcggtcagt
    49021 ttttcagccc aggctttggt ttccggtaaa tccaccaccg gttggctgta gtgaccacgg
    49081 ttgtccgggg cgacaggcgt agtggcgtcg ataatcagct tgtcggtgat ccccgcaggg
    49141 cttgagcctg gatcgagttc cagcacggac atattcggca actgcaccaa atcccctgcc
    49201 gggttcactt tcgaggagag cgcccacatc acctgcggca ggttgaacgg gtcaacgtct
    49261 tcatcgacca taatcaccat cttcacgtag cccagaccat gcggcgtggt cattgcgcgc
    49321 aggcccaccg cgcgggcaaa gccgccgtag cgttttttgg tggagataat cgccagcagg
    49381 ccatgggtgt acatggcgtt taccgcctgc acttcaggga actcggcttt cagctgctga
    49441 tacagcggca cgcaggtggc tggccccatc aggtagtcga tttcggtcca cggcataccg
    49501 aggtacagcg attcgaaaat cggcctggtg cggtaagaga ctttatcgat gcgcaccacg
    49561 gtcatgttac gcccgccgga gtagtgcccg gtaaactcac cgaacggccc ttcgatttcg
    49621 cgtttacggc tttcgatgac cccttcgagg atcacttctg aaccccacgg cacatcaaaa
    49681 ccggtcaacg gggcggtggc gatcgggtac gggctttcac gcagcgcgcc tgccatttcg
    49741 tactcggact gatcatattt cagcggcgtg gcccccatca gcgtgatgat cggatcgtta
    49801 ccgagcgtga tcgcaatcgg cagatcttca ccgcgctctt ctgctttatg cagatgcagg
    49861 gcgatatcgt gcatcggcac cggttgcagg ccgagcttac gcttgccctt cacttccatg
    49921 cggtagatgc cgacgttctg cttgccgaag ttatccgggt cgagcggatc gcgggaaacc
    49981 acgcacgctt tgtcgagata gaaaccgcca tcgccatcgt ttaaacgaaa cagcggcagg
    50041 atatcgaaca ggttgatctc gtcgccatca acggtgttct gcgcctaggc tggattggcg
    50101 cggcgctccg gggcaatcgg gaagttatcc cagcggcgga taaactcatc aatctgcttt
    50161 ttaaccgggg catttggcgg caggccgagg gaaatcgcgt ggttctgcca ggaaccgatg
    50221 gtgttcatcg ccacgcgggc atcggtaaag ccgcgaatat tatcaaacca cagcgcgggc
    50281 gcgccgtcgc cgatacgccc ggtggcgtta gctgctgctg ccagatccgg ctcggcgttc
    50341 acttcttcgc tgattttcag taactggccg tggtcatcaa gctcctgtaa aaagctgcgt
    50401 aaatcatcaa atgccattat tcattctcct gagaaaaatt ccgggcctgc ggcaatcctt
    50461 gccagcgcct ggcgtgggga tgttcgaggc caaattgatc cagcacgcgg gctaccacat
    50521 ggtggacaat gtcatctacc gtttcgggat ggttataaaa ggcaggcatc ggcggcacca
    50581 tcgccacgcc catgcgtgaa agtgcgagca tattttcgag atggatggtg ctaagcggca
    50641 tttcacgcgg caccagcacc agtttgcggc cttctttgag cacgacgtcc gccgcgcgcc
    50701 ctaccaggcc atcggcgtaa ccagcgcgga taccggcgag cgttttcata ctgcacggaa
    50761 taacgatcat gccgtcggta cgaaaagaac cggatgagat gatcgccgcc tgatccgccg
    50821 ggttatggct gaagtctgcg agggcagcaa catcgcgagc gctgtaaggc gtttccagtt
    50881 caatggtggt tttcgcccac ttcgacatca ccagatgagt ctcgacattc ggcatctccc
    50941 gcagcgcttg cagtaatgcc acaccaagag gcgcaccggt agcccctgtc atcccgacga
    51001 tcagtttcat tgcttatcct cttaattgtt cgtatacgaa cgttattatt aacatactcc
    51061 ttcatttcca cagcggcaag gattgaaaag taacaacatc ttcttcgtca ctaaaaaacc
    51121 gctatgatag cgacagagaa ttagcccgga gtttacatgg cgttacgaaa taaagcgttc
    51181 catcagttac gacagctttt tcagcagcac acggctcgct ggcagcacga gttacctgac
    51241 ctgaccaaac cacagtacgc ggtgatgcgc gccattgctg ataaacctgg tatcgaacag
    51301 gtggcactga tagaagcagc tgtcagcacc aaagcaacgt tagcagaaat gttggcaaga
    51361 atggaaaatc gtggcttagt cagacgtgaa catgaccccg ctgataagcg gcgacgcttt
    51421 gtctggttga ctgccgaagg cgaaaagata cttgctgcgg cgatacccat tggtgacagc
    51481 gtggatgagg aatttttggg gcgtttgagt ggagaagagc aggagctgtt tatgcagctg
    51541 gtgcgcaaga tgatgaacgc atagggggca aatttccgga tgcgacgcta tcgcgtctta
    51601 tccagactac aaatacaccg actcaccaac cgaaagaaaa aaggccagcc tcgcttgaga
    51661 ctggcctttc tgacagatgc ttacttactc gcggaacagc gcttcgatat tcagcccctg
    51721 cgtttgcagg atttcgcgca aacggcgcag gccttcaacc tgaatctggc gaacacgttc
    51781 acgggtgagg ccaatttcac gacctacatc ttccagtgtt gccgcttcgt accccagcaa
    51841 accgaatcga cgtgccagca cttcacgctg tttggcgttc agctcgaaca gccatttgac
    51901 gatgctctgc ttcatatcgt catcttgcgt ggtatcttcc ggaccgttct ctttttcatc
    51961 ggccaggatg tccagcaacg ctttttcgga atcaccaccc agcggggtgt ctaccgaggt
    52021 aatgcgctcg ttaagacgaa gcatacggct gacgtcatca actggcttat ccagttgctc
    52081 tgcgatctct tccgcacttg gttcatggtc cagcttatgg gacaactcac gtgcggttcg
    52141 caggtaaacg ttcagctcct ttacgatgtg aatcggcaaa cgaatagtac gggtttggtt
    52201 cataatcgcc cgttcaatcg tctggcgaat ccaccaggtt gcgtatgttg agaagcggaa
    52261 accacgttcc gggtcaaact tctctaccgc gcggatcagc cccaggttgc cctcttcgat
    52321 aaggtccagc aacgccagac cacgattgcc ataacggcgg gcaattttta ccaccagacg
    52381 caagttactc tcgatcatcc ggcggcgaga ggcgacatct ccacgcagtg cgcgacgcgc
    52441 aaaataaact tcttcttcgg ccgttaacag tggtgaataa ccaatctcac caaggtaaag
    52501 ctgagtcgcg tccaacacac gctgtgtggc tccctgcgat aacagttcct cttcggccaa
    52561 atcgttatca ctgggttcct cttctactaa ggccttttcg tcaaaaacct caactccgtt
    52621 ctcatcaaat tccgcatctt catttaaatc atgaactttc agcgtattct gactcataag
    52681 gtggctccta cccgtgatcc cttgacggaa cattcaagca aaagcatggt tccgccgatt
    52741 tatcgctgcg gcaaataacg cagcgggttt acggatttcc ccttgtaacg aatttcaaaa
    52801 tgcaagcgtg ttgaactggt tccggtgcta cccatggttg ctattttttg ccccgccttc
    52861 acttcttgtt gttcccggac cagcattgtg tcgttatggg cgtaggcact caggtaatca
    52921 tcattatgtt tgatgataat cagattaccg tagccgcgca gcgcgttacc agcataaaca
    52981 acgcggccat ctgcggtcgc gataattgcc tgtcctttgc tgcctgcgat atcaatcccc
    53041 ttgttgcccc cctcagaagc gccaaaggtt tcgatcactt tgccctcagt cggccagcgc
    53101 caggtggaga taggcgtact ggttgatgta ctgctgacag ttggctcggt tgtgcttgct
    53161 gttggtaccg ttacaggcgc tgtgaccgtg gtcgcagctg gcttgttgtt cggcaacatt
    53221 ttgttagcac tctgttcacc cgaagattca gaatacgtaa ttgtcggttg cgacgcaaca
    53281 gcaacggtgg aattttgtgc aggcttgatc acaactcctt gctctgctgc atcggcctgg
    53341 gtaatggcat ttccgccagt gattggcgta ccggaagcat tacccacctg taaggtctga
    53401 ccgacgttca gcgcgtatgg tgcctgaata ttgttgcgct gagcaaggtc acggaaatcg
    53461 ttgccagtaa tccaggcgat atagaaaagt gtgtcgcctt ttttcacggt ataggtactg
    53521 ccgctataac tgcctttcgg aatgttccca tactgacggt tatagacgat gcgtccgttt
    53581 tccatctgta ccggctgctg agctactggc tgtactggct ggatttgcgg ttgttgagta
    53641 gcctgaattt gtggctgctg taccggctga atttgcggtt gctgcgctgt agacgtcgtt
    53701 cccattttcg gcggcggcgt aatcaacata ccagaattgg tatttgcagg cgcattgcca
    53761 ttaacggagc tgaccggtgc cggtggattt gaagtgtcag aacagcctgc cagccatagc
    53821 gaaaccagtg acaaagccgc aatgcggcga acggtgaatt ttgggcttcc cgcgctcatt
    53881 tatcccccag gaaaaattgg ttaataacca gtgacataat taccgtgcaa ggcaccatac
    53941 tgaacactgg aaaagatgtt cacgatacgc tgacctgcgg caaaataacc aggaaaaatc
    54001 caggtatttc ctcacgtttt aagccagctc acccttcact aaagggacaa agcgcacggc
    54061 ctccacggta tcgataataa attcgcctcc ccgacgacgc acccgtttca aatactggtg
    54121 ctcctcccct acgggtaaga cgagaatccc gccttcgtcc agctgcgtca ttagcgcagt
    54181 tggaatttcc ggcggtgccg ccgtaacaat gatagcgtca aacggcgcac gtgcctgcca
    54241 accttgccat ccatcgccat gacgggttga aacattatgt aaatcaagat ttttcaggcg
    54301 gcgacgcgcc tgccactgca agcctttaat ccgttcaacc gagcaaacat gctggacaag
    54361 atgcgccagg attgccgttt gatatcccga accagtgcca atttccagca cccgcgactg
    54421 cggcgtcagc tcgagtaatt cggtcattcg cgccaccata tacggctgcg aaattgtctg
    54481 cccctgacct atcggcaagg cgatattgtc ccaggctttt tgttcaaacg cttcatcaac
    54541 gaatttttca cgcggcacgg cggcaagtgc attcagcacc tgctcatcct gaataccttg
    54601 cgcacgtaat tgatccagaa gtgcttgtac gcgtctgctt accattgcgt gccaactccc
    54661 acgctgttta accagtctga aaccacatct tgcgcgctat gcgcagttaa atccacatgc
    54721 agcggcgtga tggagacata gccctcatct accgcagcaa aatcggtccc cggaccggca
    54781 tcacatttac cgcccggcgg gccaatccag tacagcgtat tgccgcgcgg atcttgctgc
    54841 gggatcacct gatctgccgg atgtcgtgta ccgcagcgcg tcacgcgaat acctttgatt
    54901 tgatccaagg gtaaatccgg aacgttaata ttaagaatac gcccggtgcg caacggctct
    54961 ttacacagtg cgcgcaaaat tgaacaggtt accgccgcgg cagtgtcgta atgtttatgc
    55021 ccgtcaagcg agacggcaag cgccggaaaa cctaaatgac ggccttccat cgcggcggct
    55081 accgtaccgg aataaataac gtcatccccc agattcggcc cggcgttaat tccggacaca
    55141 acaatgtccg ggcgcggacg catcagagca ttcacgccaa gatagacgca atcggtcggg
    55201 gttcccattt gcacagcaat atcaccattt tcaaaggtaa acgtgcgcag ggaggattcc
    55261 agtgtcagag aatttgaagc gccgctgcgg ttacgatcgg gggcgaccac ctgaacgtca
    55321 gcaaactcac gcaaggcttt cgccagcgtt tgtataccgg gtgcatgtac cccgtcatca
    55381 ttactcagca atatgcgcat aatcacctgt tgtgttgata agttccctga caacgctggt
    55441 tgcaaaacta cccgccggaa gccagaaacg gatctctacg gtgacgtcat cccaccaatt
    55501 ccagcttaat tgttgcggat acagcaacat cgctctgcgc gcggcttcaa ctttttcgcg
    55561 caccagtaaa gcttgtaatt cagtttctgc ggcgacagct gccttttcga atgccagcgc
    55621 ttcacgctga gttccccatt cgccactgcc cggcaatgcg gcggttatca tcagctcttt
    55681 atcgttgacg cgacgctgta attccgccag ttcttcggtg gttgctacaa accagctacc
    55741 acgtccggct aattgtagcg catcgccgtc aacaacttga ttaacgtctg cttttttgag
    55801 gcgctcagca acaatctgat taaacaacgc actgcgggct gccgacaacc aaaaactccg
    55861 tttattgcga tcgcgcaccg gagtattggt ttgcgcccag cgcagcgcgc cctgcaagtt
    55921 gctaccgcca atcccaaaac gttgggcacc gaagtagttc ggtacacctt ttacgcaaat
    55981 atcgatcaga cgttgttcaa cgtcatcgcg attgctcact tcgcgcagaa ccagggtaaa
    56041 ggcgttacct ttcagcgcgc ctaaacgcag cttgcgcttg tgccgcgcat actccagcac
    56101 ctggcagcct tccagttgaa aggcgctcag atcgggcatt tccttgcctg gcacgcgagc
    56161 gcataaccac tgttccgtaa cagcatgttt gtctttttgc ccagcgaagc tgacttcacg
    56221 ggcatgaatt ttcaggaatt tcgccagtgc atccgccaca aaacgggtat tgcagccgtt
    56281 tttgaggatt ctaaccagaa tatgctcacc ttcaccatca ggctcaaagc ccaaatcttc
    56341 caccaccaca aagtcttccg gattggcttt cagcagcccg gtgccttgcg gtttaccgtg
    56401 gaggtaagtg agattatcaa actcaatcat tttgttgcct taatgagtag cgccaccgct
    56461 tcacaggcaa tcccttcccc acgtccggta aatccaagtt tttccgtagt agtggctttc
    56521 acgttaacat catccatatg gcagccgaga tcttcggcaa taaacacgcg catttgtgga
    56581 atgtgcggca acatcttcgg tgcctgagcg atgatagtga catcgacgtt gcccagggta
    56641 taacccttcg cctgaatacg acgccaggct tcgcgtagca gctcgcggct gtcggcacct
    56701 ttaaatgccg gatcggtatc cgggaacagc ttgccgatat cccccagcgc cgccgcgcca
    56761 agcaatgcat cggtcaacgc atggagcgcc acgtcgccat cagaatgcgc cagcaatcct
    56821 ttttcgtaag gaatgcgtac gccaccaatg ataattgggc cttcaccgcc aaaggcatgt
    56881 acgtcaaaac cgtgtccaat tcgcattatg tattctcctg atggatggtt cgggtgaggt
    56941 aaaactcggc cagtgccaaa tcttccgggc gcgtgacttt aatgttatcc gcacggcctt
    57001 cgaccaactg aggatggaat ccgcaatatt ccagcgccga ggcttcgtcg gtaatagtcg
    57061 cgccttcatt tagagcgcgc gtcagacagt catgtaacag ctcacgaggg aaaaattgcg
    57121 gcgtcagcgc gtgccataag ccgttgcgat caacggtatg agcaatggca tttttgcccg
    57181 gttcggcacg tttcatcgta tcgcgcactg gtgcggctag gatccctccc gtgcggctgg
    57241 tttcgctcaa cgccaacaat cgcgcgaggt catcctgatg cagacaagga cgagcggcgt
    57301 catgcaccaa tacccactgc gcgtcgccag cggctttcaa acctgccagc acggaatcgg
    57361 cacgctcatc accgccatct acaacggtga tttgcggatg attcgccaga ggaagttgtg
    57421 caaaacggct atcgccagga cttatggcaa tgacgacacg tttcacccgg ggatgcgcca
    57481 gcagcgcatg caccgagtgt tcaagaatgg tttgattacc gattgagaga tattgcttag
    57541 gacattccgt ttgcattcga cggccaaatc cggccgccgg aaccacggcg caaacatcca
    57601 aatgagtggt tgccatgtta attcccgggc tgatttatcg attgttttgc cccgcagact
    57661 gtgcgcgctt cgacgcgtca ggcaccagac gataaaaagt ttcgcccggc ctggtcatgc
    57721 tgagttcatt acgcgcacgc tcttcgagcg cctcctggcc gccattgaga tcgtcaattt
    57781 cggcaaaaag ttgatcgttt cgcgctttaa gtttcgcgtt tgtagcttgc tgtgccgcca
    57841 catcatcatt gacgcgggta tagtcatgta taccgttctt accgaaccac agcgaatact
    57901 gtagccagac cagaatagcc agcaacagca gcgttagttt acccatcctg ccccctgaaa
    57961 aacggcatca tcatcccatg catccgaaga cgactctaca tcctctgttg gggataccgc
    58021 gacaacgcgg gcaaatgtac cacatttgtc cattgttacg tatacccagg gcgtgcagaa
    58081 cataatctca ttattagtta cggtttgaat tatgaacaga ggagacggga aagtacaaat
    58141 tagcccagta gccacataaa cagtgcgcca aacataatgc ctactgtcac cagggtgaaa
    58201 acaatactgt agcgtagctt tccgtccatc aatgaatgca gcgcaatccc caccactacc
    58261 gcgacgggca tcagcgccag aaagaaaggc caggtgtaga taaagaagaa cagcgtgtta
    58321 gagccataaa tcaacatcgg catcgccagc gcaaataacc aggagataaa accgaccacg
    58381 gcaccaggca gtgaccatgt ggtttcttca tcctcagtaa ggctgtcgtt atttgttagt
    58441 gtaatgttat ggctattacg catatttgat cctgttactt tgacgaaccg ggcatggaaa
    58501 cccggtggtg tctcaggatc tgataatatc gttctgtctc aacagatcta ataattgctg
    58561 taccaaattt gttactaatt gttcaccatt gagatgaatt tctgccgatt caggcgcttc
    58621 gtaaacggaa tctattcccg taaagttgcg cagttcaccg gcacgcgctt tcttatataa
    58681 gccttttgga tcgcgggctt cgcaaatcgc cagcggcgta tcgacaaaca cttcgataaa
    58741 gcgcccttct cctacgcgtt cgcgaaccat ctggcgttcg gcgcggtgtg gcgagataaa
    58801 tgcggtcagc actaccagtc cggcttcaac catcaaattc gccacttcac cgacgcgacg
    58861 gatattctct ttacgatcgg catcgctaaa accgagatcg ctgcataatc cgtggcgaac
    58921 attatcgcca tccagcagat acgtactgac gccgagttta tgtaacgcct cctccagcgc
    58981 cccggcgacc gttgatttac cggacccgga gaggccggta aaccacagca ctacaccacg
    59041 atgaccgtgg tgtagctcgc gttgttgcac agtgaccgga tggctatgcc agacgacgtt
    59101 ttcgtcatgc agcgccatta tttatccccc agcaaatcgc gcgcgcccca gtgcggaaag
    59161 tggcgacgaa ccagagcatt caattccagt tcgaatgcac tgaattcaga tggcgcagca
    59221 gttgcctggc taactggctc gtgcaccata ccggcaccca cggtcacatt gctcaggcga
    59281 tcgataaaaa tcagcccacc cgtcaccgga ttttgttgat aacgatctaa caccagcggc
    59341 tcgtcaaaag tgagatccac gaggccgatc ccattcagtg gcaggttttc aacttcacgc
    59401 tgggtaaggt tattaatatc aacctgatag cgaatgccat caacacgcgc gcgcgtcttc
    59461 ttaccggcaa ttttgatgtc gtaactctgc cctggagaaa gcggctgttc cgccatccat
    59521 accacatcca ccgacgcgct ctgcaccgcc ggtaacgctt cgtctgccgc cagcagcaga
    59581 tcgccacggc tgatgtcgat ctcatccgtc agcaccaggg tgatcgcttc tccggcaaag
    59641 gcttcttcgc gatcaccatc aaaagtcacg atccgcgcga cgtttgattc cacaccagaa
    59701 ggcagcactt ttacacgttg cccgacttcc acgcgaccgg atgccagcgt tccggcgtaa
    59761 ccacgaaaat cgagattcgg gcgattaacg tactgcaccg ggaagcgcat tggctgagca
    59821 tccaccactc gctggatctc cacggtttcc agcacttcga gcagtgtcgg accgctgtac
    59881 cacggcatac tttcactttg cgatgccacg ttgtcgcctt ccagtgcaga gagcggcaca
    59941 aagcggatat ccagattacc cggcagctgc ccggcaaagg tcaaataatc ttcacgaata
    60001 cgggtgaacg tctcttcact gtaatccacc agatccattt tgttgatcgc cacgaccaga
    60061 tgtttgatcc ccaacagtgt ggagataaaa ctgtgacgac gggtttgatc gagcacgcct
    60121 ttacgggcat cgatcagtaa gatcgccagt tcacatgtcg atgcgccagt cgccatattg
    60181 cgggtgtact gctcgtgccc tggggtgtcg gcgataataa atttacgctt ctcggtagag
    60241 aaatagcggt aggccacgtc aatggtgatg ccctgttcgc gctcagcttg caggccgtcc
    60301 accagcagag ccagatccag cttttcgccc tgggtgccgt gacgcttact gtcgttatgc
    60361 agcgatgaga gctgatcttc gtagatttgg cgggtatcgt gcagcagacg accaatcaga
    60421 gtacttttgc cgtcatcgac gctaccacag gtcagaaaac gcagcaggct tttatgttgt
    60481 tgcgcaatca tccaggcttc gacgccgcct tcattggcga tttgttgtgc aagtgcggtg
    60541 ttcatcttaa aaatacccct gacgtttttt cagctccata gaccccgcct ggtcgcggtc
    60601 aatcacgcgg ccctgacgtt cactggtggt ggaaaccagc atctcttcga tgatttccgg
    60661 cagtgtttgt gcatttgact ccaccgcacc ggtcagcggc cagcagccca gcgtacggaa
    60721 acgcaccatc cgttttttaa tcacttcgcc cggttgcagg tcgatacggt tgtcatcaat
    60781 catcatcaac ataccgtcgc gttccagaac cggacgttcc gcagcgagat atagcggaac
    60841 aatgtcgata ttttccagcc agatgtattg ccagatatcc tgctcggtcc agttagagag
    60901 cgggaagacg cggatgcttt cgcctttgtt aatttgcccg ttgtagttgt gccacagctc
    60961 cgggcgctga tttttcggat cccagcgatg gaagcggtca cggaaagagt aaattcgctc
    61021 tttagcgcgg gatttctctt cgtcacggcg cgcaccaccg aaggcggcat caaaaccgta
    61081 tttgttcagc gcctgtttca ggccttcagt tttcataata tcggtatgtt tcgcgctgcc
    61141 gtgcacgaat ggattaatcc ccatcgccac gccttccggg tttttatgca ccagcagttc
    61201 gcagccgtag gctttagcag tacgatcgcg gaactcatac atctcgcgga atttccagcc
    61261 ggtatcgaca tgcagcaacg ggaaaggcag cgtacctgga taaaacgcct tgcgcgccag
    61321 atgcagcatg acgctggaat ctttaccgat agagtagagc atcaccggat ttgagaattc
    61381 tgccgccacc tcgcgaataa tgtggatgct ttccgcctcc agttgccgca ggtgagtaag
    61441 tcgtttttga tccataaccg ttcctttgca ataccgctat tttcttgcca tcagatgttt
    61501 cgactatagg gagcgtaaga gaacgaatga aattaccaat tagaatgagt agttccttaa
    61561 cggaataacg atttggcaaa gctaatatca aaaagtgctt aaggcaccgg atttcgggcg
    61621 tttaggaaga tttgaaattg ttttagcgca gcggcagttt catactatgg cggtaaaaaa
    61681 atttgcatgg tatttaagga ctcactatgt tttccgcatt gcgccaccgt accgctgccc
    61741 tggcgctcgg cgtatgcttt attctccccg tacacgcctc gtcacctaaa cctggcgatt
    61801 ttgctaatac tcaggcacga catattgcta ctttctttcc gggacgcatg accggaactc
    61861 ctgcagaaat gttatctgcc gattatattc gccaacagtt tcagcaaatg ggttatcgca
    61921 gtgatattcg gacatttaat agtcggtata tttataccgc ccgcgataat cgtaagagct
    61981 ggcataacgt gacgggaagt acggtgattg ccgctcatga aggcaaagcg ccgcagcaga
    62041 tcatcattat ggcgcatctg gatacttacg ccccgctgag cgatgctgac gccgatgcca
    62101 atctcggcgg gctgacgtta caaggaatgg atgataacgc cgcaggttta ggtgtcatgc
    62161 tggaattggc agaacgcctg aaaaatacgc ctaccgagta tggtattcga tttgtggcga
    62221 ccagcggcga agaggaaggg aaattaggcg ctgagaattt actcaagcgg atgagtgaca
    62281 ccgaaaagaa aaatacgctg ctggtgatta atctcgataa cttaattgtt ggcgataaat
    62341 tgtatttcaa cagcggtgta aaaacccctg aagcagtaag gaaattaacg cgcgacaggg
    62401 cgctggcaat tgcgcgcagt cacggaatag ccgcaacgac caatccgggt ttgaataaaa
    62461 attatccgaa aggcactggg tgttgtaatg acgcagaaat attcgacaaa gcgggcattg
    62521 ctgtactttc ggtggaagcg actaactgga atcttgggaa taaggatggt tatcagcaac
    62581 gcgcaaaaac agccgcattc cctgcgggaa atagctggca tgacgtaaga ctggataatc
    62641 aacagcatat tgataaggca cttcctggaa gaatagaacg tcgctgccgt gacgttatgc
    62701 ggataatgct accgttggtg aaggagctgg cgaaggcgtc ttgatgggtt tgaaaatggg
    62761 agctgggagt tctaccgcag gggcggggaa ttctaagtga tatccatcat cgcatccagt
    62821 gcgcccggtt tatccccgct gatgcgggga acacacatga atgtcggttc agaccgtgtt
    62881 tttacccggt ttatccccgc tggcgcgggg aacacagtgc tggaccattt caaattcaca
    62941 atagattcgg tttatccccg ctggcgcggg gaacacggaa
//
LOCUS       DAPHDW010000008        60343 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.008, whole genome shotgun sequence.
ACCESSION   DAPHDW010000008 DAPHDW010000000
VERSION     DAPHDW010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 60343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60343
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.008"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..97)
                     /locus_tag="RH057_000490"
     CDS             complement(<1..97)
                     /locus_tag="RH057_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XapX domain-containing protein"
                     /protein_id="HDV3126906.1"
                     /translation="MKDILFSSGVGFGIGALFTIVRLPIPVPNVLP"
     gene            complement(150..1406)
                     /gene="xapB"
                     /locus_tag="RH057_000491"
     CDS             complement(150..1406)
                     /gene="xapB"
                     /locus_tag="RH057_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine/proton symporter XapB"
                     /protein_id="HDV3126907.1"
                     /translation="MSIAMRLKVMSFLQYFIWGSWLVTVGSYMINTLHFTGANVGMVY
                     SSKGIAAIIMPGIMGIIADKWLRAERAYMLCHLVCAGVLFYAASVTDPDMMFWVMLVN
                     AMAFMPTIALSNSVSYSCLAQAGLDPVTAFPPIRVFGTVGFIVAMWAVSLLHLELSSL
                     QLYIASGASLLLSAYALTLPKIPVAEKKATTSLASKLGLDAFVLFKNPRMAIFFLFAM
                     MLGAVLQITNVFGNPFLHDFARNPEFADSFVVKYPSILLSVSQMAEVGFILTIPFFLK
                     RFGIKTVMLMSMVAWTLRFGFFAYGDPSTTGFILLLLSMIVYGCAFDFFNISGSVFVE
                     QEVDSSIRASAQGLFMTMVNGVGAWVGSILSGMAVDYFSVDGVKDWQTIWLVFAGYAL
                     FLAVIFFFGFKYNHDPEKIKHRAVAH"
     gene            complement(1466..2299)
                     /gene="xapA"
                     /locus_tag="RH057_000492"
     CDS             complement(1466..2299)
                     /gene="xapA"
                     /locus_tag="RH057_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthosine phosphorylase"
                     /protein_id="HDV3126908.1"
                     /translation="MSQVQFSHNPLFCIDIIKTYKPDFTPRVAFILGSGLGALADQIE
                     NAVAISYEKLPGFPVSTVHGHAGELVLGHLQGVPVVCMKGRGHFYEGRGMTIMTDAIR
                     TFKLLGCELLFCTNAAGSLRPEVGAGSLVALKDHINTMPGTPMVGLNDDRFGERFFSL
                     ANAYDAEYRALLQKVAKEEGFPLTEGVFVSYPGPNFETAAEIRMMQIIGGDVVGMSVV
                     PEVISARHCDLKVVAVSAITNMAEGLSDVKLSHAQTLAAAELSKQNFINLICGFLRKI
                     A"
     gene            2548..3312
                     /locus_tag="RH057_000493"
     CDS             2548..3312
                     /locus_tag="RH057_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3126909.1"
                     /translation="MKKHLLTLTLSSILAIPVVSHAEFKGGFADIGVHYLDWTSRTTE
                     KSSTKSHKDDFGYLEFEGGANFSWGEMYGFFDWENFYNGRHNKPGSEQRYTFKNTNRI
                     YLGDTGFNLYLHAYGTYGSANRVNFHDDMFLYGIGYNFTGSGWWFKPFFAKRYTDQTY
                     YTGDNGYVAGWVAGYNFMLGSEKFTLTNWNEYEFDRDATYAAGNGGKEGLNGAVALWW
                     NATSHITTGIQYRYADDKLGEDFYQDAIIYSIKFNF"
     gene            complement(3351..4277)
                     /locus_tag="RH057_000494"
     CDS             complement(3351..4277)
                     /locus_tag="RH057_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3126910.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            4367..5365
                     /locus_tag="RH057_000495"
     CDS             4367..5365
                     /locus_tag="RH057_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HDV3126911.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVICSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGAGGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLGFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(5362..5580)
                     /locus_tag="RH057_000496"
     CDS             complement(5362..5580)
                     /locus_tag="RH057_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HDV3126912.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(5582..7597)
                     /gene="ligA"
                     /locus_tag="RH057_000497"
     CDS             complement(5582..7597)
                     /gene="ligA"
                     /locus_tag="RH057_000497"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HDV3126913.1"
                     /translation="MESIEQQLTELRTTLRHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGLPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKSAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(7668..8654)
                     /gene="zipA"
                     /locus_tag="RH057_000498"
     CDS             complement(7668..8654)
                     /gene="zipA"
                     /locus_tag="RH057_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HDV3126914.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYQPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            8884..9645
                     /gene="cysZ"
                     /locus_tag="RH057_000499"
     CDS             8884..9645
                     /gene="cysZ"
                     /locus_tag="RH057_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HDV3126915.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTLMSYVPDWLQWLSYLLWPLAVISVLLVFGSFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFAWYLPRAIVLLILYLIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            9830..10801
                     /gene="cysK"
                     /locus_tag="RH057_000500"
     CDS             9830..10801
                     /gene="cysK"
                     /locus_tag="RH057_000500"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HDV3126916.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQALAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            11185..11442
                     /gene="ptsH"
                     /locus_tag="RH057_000501"
     CDS             11185..11442
                     /gene="ptsH"
                     /locus_tag="RH057_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HDV3126917.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            11487..13214
                     /gene="ptsI"
                     /locus_tag="RH057_000502"
     CDS             11487..13214
                     /gene="ptsI"
                     /locus_tag="RH057_000502"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HDV3126918.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            13255..13764
                     /gene="crr"
                     /locus_tag="RH057_000503"
     CDS             13255..13764
                     /gene="crr"
                     /locus_tag="RH057_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HDV3126919.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(13806..14657)
                     /gene="pdxK"
                     /locus_tag="RH057_000504"
                     /gene_synonym="thiJ"
     CDS             complement(13806..14657)
                     /gene="pdxK"
                     /locus_tag="RH057_000504"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HDV3126920.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            14762..15130
                     /locus_tag="RH057_000505"
     CDS             14762..15130
                     /locus_tag="RH057_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HDV3126921.1"
                     /translation="MKKIICLVITLLMTLPAYAKLTAHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAQ"
     gene            complement(15133..16044)
                     /gene="cysM"
                     /locus_tag="RH057_000506"
     CDS             complement(15133..16044)
                     /gene="cysM"
                     /locus_tag="RH057_000506"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HDV3126922.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSRFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNASLVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(16178..17275)
                     /gene="cysA"
                     /locus_tag="RH057_000507"
     CDS             complement(16178..17275)
                     /gene="cysA"
                     /locus_tag="RH057_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HDV3126923.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNIAFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPEQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQRGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(17265..18140)
                     /gene="cysW"
                     /locus_tag="RH057_000508"
     CDS             complement(17265..18140)
                     /gene="cysW"
                     /locus_tag="RH057_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HDV3126924.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(18140..18973)
                     /gene="cysT"
                     /locus_tag="RH057_000509"
     CDS             complement(18140..18973)
                     /gene="cysT"
                     /locus_tag="RH057_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HDV3126925.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLAQMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(18973..19989)
                     /gene="cysP"
                     /locus_tag="RH057_000510"
     CDS             complement(18973..19989)
                     /gene="cysP"
                     /locus_tag="RH057_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HDV3126926.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKNKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRN"
     gene            complement(20160..20951)
                     /gene="ucpA"
                     /locus_tag="RH057_000511"
     CDS             complement(20160..20951)
                     /gene="ucpA"
                     /locus_tag="RH057_000511"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HDV3126927.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DDDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPM
                     RRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(21080..21937)
                     /gene="murR"
                     /locus_tag="RH057_000512"
     CDS             complement(21080..21937)
                     /gene="murR"
                     /locus_tag="RH057_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HDV3126928.1"
                     /translation="MLYLTKISNAGSEFTENEQKIADFLQANVSELQSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCSLFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQLNDVESLKMIQR
                     SSELTQRLK"
     gene            22101..22997
                     /gene="murQ"
                     /locus_tag="RH057_000513"
     CDS             22101..22997
                     /gene="murQ"
                     /locus_tag="RH057_000513"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HDV3126929.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPLAVERV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            23001..24425
                     /gene="murP"
                     /locus_tag="RH057_000514"
     CDS             23001..24425
                     /gene="murP"
                     /locus_tag="RH057_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HDV3126930.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGEAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFITACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            24430..25734
                     /gene="pbp4b"
                     /locus_tag="RH057_000515"
     CDS             24430..25734
                     /gene="pbp4b"
                     /locus_tag="RH057_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin binding protein PBP4B"
                     /protein_id="HDV3126931.1"
                     /translation="MKRTMLYLSLLAVSCSVSAAKYPVLTESSPEKAGFNVERLNQMD
                     RWISQQVDAGYPGVNLLIIKDNQIVYRKAWGAAKKYDGSVLMEQPVKATTGTLYDLAS
                     NTKMYATNFALQKLMSEGKLHPDDRIAKYIPGFADSPNDTIKGKNTLRISDLLHHSGG
                     FPADPQYPNKAVAGALYSQDKGQTLEMIKRTPLEYQPGSKHIYSDVDYMLLGFIVESV
                     TGQPLDRYVEESIYRPLGLTHTVFNPLLKGFKPQQIAATELNGNTRDGVIHFPNIRTS
                     TLWGQVHDEKAFYSMGGVSGHAGLFSNTGDIAVLMQTMLNGGGYGDVQLFSAETVKMF
                     TTSSKEDATFGLGWRVNGNATMTPTFGTLASPQTYGHTGWTGTVTVIDPVNHMAIVML
                     SNKPHSPVADPQKNPNMFESGQLPIATYGWVVDQVYAALKQK"
     gene            complement(25792..26691)
                     /gene="yfeX"
                     /locus_tag="RH057_000516"
     CDS             complement(25792..26691)
                     /gene="yfeX"
                     /locus_tag="RH057_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311329.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HDV3126932.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDAGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(26787..27362)
                     /locus_tag="RH057_000517"
     CDS             complement(26787..27362)
                     /locus_tag="RH057_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HDV3126933.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTANGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWSGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(27423..27872)
                     /locus_tag="RH057_000518"
     CDS             complement(27423..27872)
                     /locus_tag="RH057_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HDV3126934.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRATFPRTWRVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALVWLLTNRRLRACFNEVKE"
     gene            complement(27859..28284)
                     /locus_tag="RH057_000519"
     CDS             complement(27859..28284)
                     /locus_tag="RH057_000519"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HDV3126935.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            28498..29367
                     /gene="amiA"
                     /locus_tag="RH057_000520"
     CDS             28498..29367
                     /gene="amiA"
                     /locus_tag="RH057_000520"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HDV3126936.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            29371..30270
                     /gene="hemF"
                     /locus_tag="RH057_000521"
     CDS             29371..30270
                     /gene="hemF"
                     /locus_tag="RH057_000521"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HDV3126937.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCLPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(30276..31328)
                     /gene="eutR"
                     /locus_tag="RH057_000522"
     CDS             complement(30276..31328)
                     /gene="eutR"
                     /locus_tag="RH057_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
                     /protein_id="HDV3126938.1"
                     /translation="MKKTRTANLHHLYHEPLPENLKLTPKVEVDNVHQRQTTDVYEHA
                     LTITAWQQIYDQLHPGKFHGEFTEILLDDIQVFREYTGLALRQSCLVWPNSFWFGIPA
                     TRGEQGFIGSQCLGSAEIATRPGGTEFELSTPDDYTILGVVLSEDVITRQANFLHNPD
                     RVLHMLRSQSALEVKEQHKAALWGFVQQALATFCENPENLHQPAVRKVLGDNLLMAMG
                     AMLEDAQPMVTAESISHQSYRRLLSRAREYVLENMSEPVTVLDLCNQLHVSRRTLQNA
                     FHAILGIGPNAWLKRIRLNAVRRELISPWSQSTTVKDAAMQWGFWHLGQFATDYQQLF
                     AEKPSLTLHQRMREWG"
     gene            complement(31374..31874)
                     /gene="eutK"
                     /locus_tag="RH057_000523"
     CDS             complement(31374..31874)
                     /gene="eutK"
                     /locus_tag="RH057_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HDV3126939.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVREPD
                     APVIVAESADELLALLTSVRQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(31887..32546)
                     /gene="eutL"
                     /locus_tag="RH057_000524"
     CDS             complement(31887..32546)
                     /gene="eutL"
                     /locus_tag="RH057_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HDV3126940.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            complement(32556..33443)
                     /gene="eutC"
                     /locus_tag="RH057_000525"
     CDS             complement(32556..33443)
                     /gene="eutC"
                     /locus_tag="RH057_000525"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit beta"
                     /protein_id="HDV3126941.1"
                     /translation="MDQKQIEEIVRSVMASMGQTAPAPSEAKCATTNCAAPVTSESCA
                     LDLGSAEAKAWIGVENPHRADVLTELRRSTVARVCTGRAGPRPRTQALLRFLADHSRS
                     KDTVLKEVPEEWVKAQGLLEVRSEISDKNLYLTRPDMGRRLCAEAVEALKAQCVANPD
                     VQVVISDGLSTDAITVNYEEILPPLMAGLKQAGLKVGTPFFVRYGRVKIEDQIGEILG
                     AKVVILLVGERPGLGQSESLSCYAVYSPRMATTVEADRTCISNIHQGGTPPVEAAAVI
                     VDLAKRMLEQKASGINMTR"
     gene            complement(33464..34825)
                     /gene="eutB"
                     /locus_tag="RH057_000526"
     CDS             complement(33464..34825)
                     /gene="eutB"
                     /locus_tag="RH057_000526"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit alpha"
                     /protein_id="HDV3126942.1"
                     /translation="MKLKTTLFGNVYQFKDVKEVLAKANELRSGDVLAGVAAASSQER
                     VAAKQVLSEMTVADIRNNPVIAYEDDCVTRLIQDDVNETAYNQIKNWSISELREYVLS
                     DETSVDDIAFTRKGLTSEVVAAVAKICSNADLIYGAKKMPVIKKANTTIGIPGTFSAR
                     LQPNDTRDDVQSIAAQIYEGLSFGVGDAVIGVNPVTDDVENLSRVLDTIYGVIDKFNI
                     PTQGCVLAHVTTQIEAIRRGAPGGLIFQSICGSEKGLKEFGVELAMLDEARAVGAEFN
                     RIAGENCLYFETGQGSALSAGANFGADQVTMEARNYGLARHYDPFIVNTVVGFIGPEY
                     LYNDRQIIRAGLEDHFMGKLSGISMGCDCCYTNHADADQNLNENLMILLATAGCNYIM
                     GMPLGDDIMLNYQTTAFHDTATVRQLLNLRPSPEFERWLESMGIMANGRLTKRAGDPS
                     LFF"
     gene            complement(34837..36240)
                     /gene="eutA"
                     /locus_tag="RH057_000527"
     CDS             complement(34837..36240)
                     /gene="eutA"
                     /locus_tag="RH057_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HDV3126943.1"
                     /translation="MNTRQLLSVGIDIGTTTTQVIFSHLELVNRAAVSQVPRYEFIKR
                     EISWQSPVFFTPVDKQGGLKEAELKTLILEQYQAAGIAPESVDSGAIIITGESAKTRN
                     ARPAVMALSQSLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANY
                     ALFDAGKISGTACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQL
                     VQVTRRMAELIVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPADP
                     FCFADIGPLLATTLHDHPRLREMNVQFPAQTVRATVIGAGAHTLSLSGSTIWLEGVQL
                     PLRNLPVAIPIDETDLVSAWQQALIQLDLDPKTDAYVLALPASLPVRYAAVLTVINAL
                     VDFVARFPNPHPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPL
                     FGGSVVPVTVKSLAFPS"
     gene            complement(36237..37463)
                     /gene="eutH"
                     /locus_tag="RH057_000528"
     CDS             complement(36237..37463)
                     /gene="eutH"
                     /locus_tag="RH057_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutH"
                     /protein_id="HDV3126944.1"
                     /translation="MGINEIIMYIMMFFMLIAAVDRILSQFGGSARFLGKFGKSIEGS
                     GGQFEEGFMAMGALGLAMVGMTALAPVLAHVLGPVIIPVYEMLGANPSMFAGTLLACD
                     MGGFFLAKELAGGDVAAWLYSGLILGSMMGPTIVFSIPVALGIIEPSDRRYLALGVLA
                     GIVTIPIGCIAGGLVAMYSGVQINGQPVEFTFALILMNMIPVIIVAILVALGLKFIPE
                     KMINGFQIFAKFLVALITLGLAAAVVKFLLGWELIPGLDPIFMAPGDKPGEVMRAIEV
                     IGSISCVLLGAYPMVLLLTRWFEKPLMSVGKVLNMNNIAAAGMVATLANNIPMFGMMK
                     QMDTRGKVINCAFAVSAAFALGDHLGFAAANMNAMIFPMIVGKLIGGVTAIGVAMMLV
                     PKEDATATKTEAEAQS"
     gene            complement(37580..38767)
                     /gene="eutG"
                     /locus_tag="RH057_000529"
     CDS             complement(37580..38767)
                     /gene="eutG"
                     /locus_tag="RH057_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization ethanol dehydrogenase
                     EutG"
                     /protein_id="HDV3126945.1"
                     /translation="MQNELQTALFQAFDTLNLQRVKTFSVPPVTLCGPGSVSSCGQQA
                     QTRGLKHLFVMADSFLHQAGMTAGLTRSLAVKGIAMTLWPCPVGEPCITDVCAAVAQL
                     RESGCDGVIAFGGGSVLDAAKAVALLVTNPDSTLAEMSETSVLQPRLPLIAIPTTAGT
                     GSETTNVTVIIDAVSGRKQVLAHASLMPDVAILDAALTEGVPSHVTAMTGIDALTHAI
                     EAYSALNATPFTDSLAIGAIAMIGKSLPKAVGYGHDLAARESMLLASCMAGMAFSSAG
                     LGLCHAMAHQPGAALHIPHGLANAMLLPTVMEFNRMVCRERFSQIGRALRTKKSDDHD
                     AINAVSELIAEVGIGKRLGDVGATSAHYGAWAQAAQEDICLRSNPRTASLEQIVGLYA
                     AAQ"
     gene            complement(38757..39593)
                     /gene="eutJ"
                     /locus_tag="RH057_000530"
     CDS             complement(38757..39593)
                     /gene="eutJ"
                     /locus_tag="RH057_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HDV3126946.1"
                     /translation="MAHDEQWLTPRLQTAATLCNQTPAATESPLWLGVDLGTCDVVSM
                     VVDRDGQPVAVCLDWADVVRDGIVWDFFGAVTIVRRHLDTLEQQFGRRFSHAATSFPP
                     GTDPRISINVLESAGLEVSHVLDEPTAVADLLQLDNAGVVDIGGGTTGIAIVKKGKVT
                     YSADEATGGHHISLTLAGNRRISLEEAEQYKRGHGDEIWPAVKPVYEKMADIVARHIE
                     GQGITDLWLAGGSCMQPGVAELFRKQFPALQVHLPQHSLFMTPLAIASSGREKAEGLY
                     AK"
     gene            complement(39604..41007)
                     /locus_tag="RH057_000531"
     CDS             complement(39604..41007)
                     /locus_tag="RH057_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase EutE"
                     /protein_id="HDV3126947.1"
                     /translation="MNQQDIEQVVKAVLLKMQSSDTPPAAVHEMGVFASLDDAVAAAK
                     VAQQGLKSVAMRQLAIAAIREAGEKHARDLAELAVSETGMGRVEDKFAKNVAQARGTP
                     GVECLSPQVLTGDNGLTLIENAPWGVVASVTPSTNPAATVINNAISLIAAGNSVIFAP
                     HPAAKKVSQRAITLLNQAIVAAGGPENLLVTVANPDIETAQRLFKFPGIGLLVVTGGE
                     AVVEAARKHTNKRLIAAGAGNPPVVVDETADLARAAQSIVKGASFDNNIICADEKVLI
                     VVDSVADELMRLMEGQHAVKLTAEQAQQLQPVLLKNIDERGKGTVSRDWVGRDAAKIA
                     AAIGLNVPQETRLLFVETTAEHPFAVTELMMPVLPVVRVANVADAIALAVKLEGGCHH
                     TAAMHSRNIENMNQMANAIDTSIFVKNGPCIAGLGLGGEGWTTMTITTPTGEGVTSAR
                     TFVRLRRCVLVDAFRIV"
     gene            complement(41019..41306)
                     /gene="eutN"
                     /locus_tag="RH057_000532"
     CDS             complement(41019..41306)
                     /gene="eutN"
                     /locus_tag="RH057_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutN"
                     /protein_id="HDV3126948.1"
                     /translation="MKLAVVTGQIVCTVRHHGLAHDKLLMVEMIDPQGNPDGQCAVAI
                     DNIGAGTGEWVLLVSGSSARQAHKSETSPVDLCVIGIVDEVVSGGQVIFHK"
     gene            complement(41413..41706)
                     /gene="eutM"
                     /locus_tag="RH057_000533"
     CDS             complement(41413..41706)
                     /gene="eutM"
                     /locus_tag="RH057_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutM"
                     /protein_id="HDV3126949.1"
                     /translation="MEALGMIETRGLVALIEASDAMVKAARVKLVGVKQIGGGLCTAM
                     VRGDVAACKAATDAGAAAAQRIGELVSVHVIPRPHGDLEEVFPIGLKGDSSNL"
     gene            complement(41745..42761)
                     /gene="pta"
                     /locus_tag="RH057_000534"
     CDS             complement(41745..42761)
                     /gene="pta"
                     /locus_tag="RH057_000534"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV3126950.1"
                     /translation="MIIERCRELALRAPARVVFPDALDQRVLKAAQYLHQQGLATPIL
                     VANPFELRQFALSHGVAMDGLQVIDPHGNLAMREEFAHRWLARAGEKTPPDALEKLTD
                     PLMFAAAMVSAGKADVCIAGNLSSTANVLRAGLRIIGLQPGCKTLSSIFLMLPQYSGP
                     ALGFADCSVVPQPTAAQLADIALASAETWRAITGEEPRVAMLSFSSNGSARHPCVANV
                     QQATEIVRERAPKLVVDGELQFDAAFVPEVAAQKAPASPLQGKANVMVFPSLEAGNIG
                     YKIAQRLGGYRAVGPLIQGLAAPMHDLSRGCSVQEIIELALVAAVPRQTEVNRESSLQ
                     TLVE"
     gene            complement(42758..43561)
                     /gene="eutT"
                     /locus_tag="RH057_000535"
     CDS             complement(42758..43561)
                     /gene="eutT"
                     /locus_tag="RH057_000535"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization cob(I)yrinic acid
                     a,c-diamide adenosyltransferase EutT"
                     /protein_id="HDV3126951.1"
                     /translation="MKDFITEAWLRANHTLSEGAEIHLPADSRLTPSARELLESRHLR
                     IKFIDEQGRLFVDDEQQQPQPVHGLTSSDEHPQACCELCRQPVAKKPDTLTHLSAEKM
                     VAKSDPRLGFRAVLDSTIALAVWLQIELAEPWQPWLADIRSRLGNIMRADALGEPLGC
                     QAIVGLSDEDLHRLSHQPLRYLDHDHLVPEASHGRDAALLNLLRTKVRETETVAAQVF
                     ITRSFEVLRPDILQALNRLSSTVYVMMILSVTKQPLTVKQIQQRLGETQ"
     gene            complement(43558..44259)
                     /gene="eutQ"
                     /locus_tag="RH057_000536"
     CDS             complement(43558..44259)
                     /gene="eutQ"
                     /locus_tag="RH057_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutQ"
                     /protein_id="HDV3126952.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLEQGAMQPSFKSVTGKGGIKVIDGSSVKFGRFDGAEPHCVGLTDLVTGDDGSSMAAG
                     FMQWENAFFPWTLNYDEIDMVLEGELHVRHEGETMIAKAGDVMFIPKGSSIEFGTTSS
                     VKFLYVAWPANWQSL"
     gene            complement(44234..44713)
                     /gene="eutP"
                     /locus_tag="RH057_000537"
     CDS             complement(44234..44713)
                     /gene="eutP"
                     /locus_tag="RH057_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HDV3126953.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTQAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(44726..45061)
                     /gene="eutS"
                     /locus_tag="RH057_000538"
     CDS             complement(44726..45061)
                     /gene="eutS"
                     /locus_tag="RH057_000538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HDV3126954.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(45354..47633)
                     /gene="maeB"
                     /locus_tag="RH057_000539"
     CDS             complement(45354..47633)
                     /gene="maeB"
                     /locus_tag="RH057_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HDV3126955.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGDQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLLSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            47922..48872
                     /gene="tal"
                     /locus_tag="RH057_000540"
     CDS             47922..48872
                     /gene="tal"
                     /locus_tag="RH057_000540"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV3126956.1"
                     /translation="MNELDGIKQFTTVVADSGDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGRVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     AQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            48892..50895
                     /gene="tkt"
                     /locus_tag="RH057_000541"
     CDS             48892..50895
                     /gene="tkt"
                     /locus_tag="RH057_000541"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV3126957.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKKIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFTRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQVEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(50991..52034)
                     /locus_tag="RH057_000542"
     CDS             complement(50991..52034)
                     /locus_tag="RH057_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HDV3126958.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQMIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDAWPTLWITR"
     gene            complement(52160..52735)
                     /gene="nudK"
                     /locus_tag="RH057_000543"
     CDS             complement(52160..52735)
                     /gene="nudK"
                     /locus_tag="RH057_000543"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HDV3126959.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQTSHLMD"
     gene            complement(52803..54782)
                     /gene="aegA"
                     /locus_tag="RH057_000544"
     CDS             complement(52803..54782)
                     /gene="aegA"
                     /locus_tag="RH057_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HDV3126960.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKCGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVGVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDSLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVRNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPVEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            54988..56688
                     /gene="narQ"
                     /locus_tag="RH057_000545"
     CDS             54988..56688
                     /gene="narQ"
                     /locus_tag="RH057_000545"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
                     /protein_id="HDV3126961.1"
                     /translation="MIVKRPVSASLARAFFYIVLLSILSTGIALLTLASSLRDAEAIN
                     IAGSLRMQSYRLGYDLQSGSPQLNAHRQLFQQALHSPVLTNLNVWYVPEAVKTRYAHL
                     NANWLEMNNRLSKGDLPWYQANINNYVNQIDLFVLALQHYAERKMLLVVAISLAGGIG
                     IFTLVFFTLRRIRHQVVAPLNQLVTASQRIEHGQFDSPPLDTSLPNELGLLAKTFNQM
                     SSELHKLYLSLEASVEEKTRDLHEAKRRLEVLYQCSQALNTSQIDVHCFRHILQIVRD
                     NEAAEYLELNVGENWRISEGQPNPELPMQILPVTMQETVYGELHWQNSHVSSSEPLLN
                     SVSSMLGRGLYFNQAQKHFQQLLLMEERATIARELHDSLAQVLSYLRIQLTLLKRSIP
                     EDNATAQSIMADFSQALNDAYRQLRELLTTFRLTLQQADLPSALREMLDTLQNQTSAK
                     LTLDCRLPTLALDAQMQVHLLQIIREAVLNAMKHANASEIAVSCVTAPDGNHTVYIRD
                     NGIGIGEPKEPEGHYGLNIMRERAERLGGTLTFSQPSGGGTLVSISFRSAEGEESQLM
                     "
     gene            56852..59965
                     /gene="acrD"
                     /locus_tag="RH057_000546"
     CDS             56852..59965
                     /gene="acrD"
                     /locus_tag="RH057_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HDV3126962.1"
                     /translation="MANFFIDRPIFAWVLAILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASGANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMILTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNKIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(60064..60123)
                     /gene="ypfM"
                     /locus_tag="RH057_000547"
     CDS             complement(60064..60123)
                     /gene="ypfM"
                     /locus_tag="RH057_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HDV3126963.1"
                     /translation="MIERELGNWKDFIEVMLRK"
BASE COUNT        14129 a        16629 c        15418 g        14167 t
ORIGIN      
        1 ctggtaatac atttggtaca ggaataggca agcgcacgat tgtaaacaaa gcaccaatgc
       61 caaaaccaac gccggaagaa aataaaatat ctttcactaa ttaaccttta tcataaaagc
      121 agctctgaag agcagagctg cgaatccttt taatgagcca ccgctcgatg ctttatcttt
      181 tcagggtcat gattatattt aaacccaaag aaaaatatca ctgcgagaaa aagagcatat
      241 cctgcaaaca ccagccagat agtttgccag tcttttacgc catccaccga aaagtaatct
      301 actgccatgc cactcagaat cgagccaacc catgcgccga caccatttac catggtcata
      361 aagagcccct gcgcgctggc acgaatgctg gaatcaactt cctgttcgac aaataccgaa
      421 ccagaaatat tgaagaaatc gaatgcacag ccataaacaa tcatcgacag cagcagcaaa
      481 ataaatccgg ttgttgacgg atcgccatag gcgaagaagc caaagcgcag cgtccaggcc
      541 accatactca tcagcatgac ggttttaatg ccaaatcgct ttaaaaagaa tgggatagtc
      601 agtataaagc ccacttctgc catctgtgaa actgacagta aaatggaggg atatttcacc
      661 acaaaactgt cagcaaactc cgggttacgg gcgaaatcat gtaggaacgg attaccaaaa
      721 acgttggtaa tttgcagtac cgcacccagc atcatggcaa agaggaaaaa gatggccatg
      781 cgtggatttt taaacagcac gaaggcatcc agacccagct tgctggcaag cgatgtggtc
      841 gcttttttct ccgcaaccgg aatcttcggc aaagtcagcg cataagccga cagcagcaat
      901 gacgcaccgg acgcgatata cagctgcaga ctactcaatt ccagatgcag caggcttact
      961 gcccacatcg cgacaatgaa ccccaccgta ccaaaaacgc gaatgggcgg gaaagcggtc
     1021 accgggtcaa gccctgcctg ggcaagacag gaataagaga cgctgttcga taacgcaata
     1081 gtcggcataa acgccatcgc attgactaac atcacccaaa acatcatatc cggatcagtt
     1141 acggatgccg cataaaaaag tacgcccgca cacaccaggt gacacagcat gtatgcacgt
     1201 tctgcgcgca gccatttgtc tgcgatgatc cccattatac caggcataat aatcgcggcg
     1261 atccctttgg aactgtaaac catgccaaca ttagcgccgg tgaaatgaag ggtattaatc
     1321 atgtaagagc cgacggtaac cagccagctc ccccagataa aatattgcaa aaaggacatt
     1381 acctttaagc gcatcgcgat gctcataatc gtttccttgt cgcatcattt ggcctcaccc
     1441 gcgaggccgt ttcttattgt ttttctcagg caattttgcg cagaaagcca caaataaggt
     1501 tgatgaagtt ctgcttagag agctccgcag ccgccagcgt ttgcgcatgt gacaatttca
     1561 catcgcttaa accttccgcc atattggtaa tcgcagagac cgcaacgact ttaaggtcgc
     1621 aatggcgagc tgaaataacc tcaggcacca cagacatacc aacaacatcc ccaccaataa
     1681 tttgcatcat gcgaatttcc gccgcagtct cgaaattcgg ccccggatac gagacgaaca
     1741 cgccctccgt cagagggaaa ccctcttctt tcgccacttt ttgtaacagt gcgcggtatt
     1801 ccgcatcgta ggcatttgcc agtgagaaga agcgctctcc aaaacgatca tcgttaagac
     1861 ccaccatcgg cgtaccgggc atggtgttga tatgatcttt caatgcgacc aggctgcctg
     1921 cccccacttc cggacgcagc gagcctgccg cattggtgca gaacagtagc tcgcagccca
     1981 gcagcttaaa ggtacgaatt gcgtcagtca taatggtcat tccacgacct tcgtagaaat
     2041 gtccgcgacc tttcatacat accaccggta cgccctggag atggcccagc actaactccc
     2101 ccgcatgacc atgtaccgta cttaccggga agcccggcag cttttcgtag gaaattgcga
     2161 cagcgttctc aatctgatcg gccagcgcgc cgagcccgga acctaaaata aaggccactc
     2221 gtggcgtgaa atcaggttta taagtcttga taatatctat gcaaaacagc gggttatgag
     2281 aaaattgaac ctgagacata catatccttt ttctgtaggg tggaatctaa cgccttttta
     2341 tagcgaggtt gtcccggctc ttaccaatac acttttctca cgcattcaat agcgattcga
     2401 tatcgatgga tttatatctt ttctctatta atccacggta aaaactgtat tggcgatatt
     2461 aattcctttg cattaacaat atgtcgtttt tagcaaacca ctttattaag caatctacta
     2521 ataacgatcg gcatagggaa ttatgctatg aaaaaacatc ttttaactct gacactttcc
     2581 tctatattag cgatacctgt tgtatctcat gcagaattta aaggcggttt tgcagatatc
     2641 ggcgtgcatt atctggactg gaccagccgg actaccgaaa aatcgtcgac caaatcacac
     2701 aaagatgatt ttggttatct tgaatttgaa ggtggcgcta actttagttg gggagaaatg
     2761 tacgggttct tcgactggga aaacttctat aacggtcgcc ataataaacc aggtagcgag
     2821 cagcgttata cctttaaaaa taccaaccgt atttacctgg gtgacaccgg atttaatctc
     2881 tacttgcatg cgtatggcac ctacggttct gcgaatcgcg tgaatttcca cgacgatatg
     2941 ttcctgtacg gtatcggcta caatttcacc ggcagcggtt ggtggttcaa accgttcttt
     3001 gctaagcgtt atacagatca aacctattac actggcgaca acggctatgt cgccggctgg
     3061 gttgcaggtt acaactttat gctgggcagc gagaaattca ctcttaccaa ctggaacgag
     3121 tacgagtttg accgtgatgc tacctatgca gcgggtaatg gcggtaaaga agggctgaat
     3181 ggcgcagttg cactgtggtg gaatgcaaca tcacacatta ctacggggat tcagtatcga
     3241 tatgcggatg acaaactggg cgaagatttt taccaggatg cgataatcta ctccatcaaa
     3301 tttaacttct gattgattac tgccggggct cccggcagta tgaattcact ctatatctga
     3361 tacaacggat ccccttcccg cccagccatt agcgcattgc cctggtctcg taccacgtcc
     3421 cataacgctt cagcggcggt ggaaagtgaa cggttcttcc tgcgcaccag catcaactgg
     3481 cgttcaacaa ccggcgtaat gcgctttacc accagtgggc taccttcagg caatggtagc
     3541 gccagtgcgg gaagaatact gatgccgatg cctgcggcta ccatcgggaa cagcgtcgcc
     3601 ggatgtccaa tctcctgcac aatattcgcc tgaataccat tgcgcgccag cgctgcgtca
     3661 atcagcggtc ggctgcctga cgcgtaatcc tgcaacacca atttcgctcc ctgtaatgcc
     3721 tgccagggta cataatcttc gacggccaaa gcgctatcgc gatggcaaag cagaaagaaa
     3781 ggctcggaaa gaatcgcttc acattgcaga tcgcccactg ggcctggatc gatgacgatg
     3841 ccaaagtcga catctccctg gcgaatactt tccatcaccc actgctgcgg acgatcgtgc
     3901 aggacaaact gaatatctgg atagcggcga tggctttcag caatgcattg cggaataaga
     3961 tgcgcggaaa tggtctggct ggcagcgacc cgaacttttc cgctcagttg ttgccccata
     4021 cgcccggtat cgcgcaacgt gctgttcagt tcatccagca gtcgctcaag acgcaaagcc
     4081 agctgctgcc ctgcatctgt aagcaccact tcacgcgtgg ttctgtccag caggcgaaca
     4141 ccagtatgat tttccagttc cttcacactg tgactcactg ccgactggct caggccgata
     4201 cgctctcctg cacgactaaa acttttctcc tgcgctactg tgacgaaaac ctttaattgt
     4261 tttaaagaat aattcatctg ttttcttcat gaatggatgc aataaatcaa ttttatttct
     4321 caaactggaa gaagcacaat agaaccatcg atcatctgga gtctttatga aactttttcg
     4381 tatcctcgat cctttcacct taaccctgat cacggtggtg ttgctggcct ctttctttcc
     4441 ggccagaggc gatttcgtcc ccttctttga aaatctgacc accgcagcga ttgccctgct
     4501 gttctttatg cacggcgcga agttgtcgcg tgaggcgatt attgctggcg gtggtcactg
     4561 gcgactgcat ttgtgggtaa tttgcagcac cttcgtgctg tttccgattc tgggtgtact
     4621 gtttgcctgg tggaaaccgg taaatgtcga cccgatgctc tactccggtt ttctctactt
     4681 gtgcattctc ccggctaccg tgcagtctgc aatcgccttc acgtcaatgg cgggcggtaa
     4741 cgtcgcggcg gcggtttgtt ctgcgtcagc atccagcctg ctggggattt tcctttcacc
     4801 attgctggtt ggtctggtga tgaatgttca cggtgcaggg ggcagccttg agcaggtcgg
     4861 taaaattatg ctgcaactgc tgctgccgtt tgtgttgggg catctttccc ggccgtggat
     4921 tggtgactgg gtgtcgcgca ataaaaaatg gattgcgaaa actgaccaga cgtccattct
     4981 gttggtggtt tatacagcgt tcagcgaagc cgtcgttaat ggtatctggc ataaagttgg
     5041 ctggggatca ttgctgttta tcgtggtggt cagttgcgtt cttctggcta tcgtgattgt
     5101 agttaacgtc tttatggcac gccgactggg cttcaataag gcagatgaaa ttactatcgt
     5161 cttttgtggt tcgaaaaaga gtctggcaaa tggcatcccg atggcaaaca ttctgttccc
     5221 cacatcggtg atcggtatga tggtgctgcc cctaatgatt ttccatcaga tccaattgat
     5281 ggtctgtgcg gtgctggcgc gtcgatacaa acgccagacc gaacagttac aggcacagca
     5341 ggaaagcagc gccgataaag cttaaagcgg acgcttcagg ggctggacca gttgcgtcag
     5401 cccctcggtt ttaatcagca gcgtgatttg cattagctca ccgagcttcc ctgccgggaa
     5461 ttcatctttg cgggcaaacc acagcagata ctcctccggc aggtcgatta agcgacgccc
     5521 tttatatttg ccaaacggca ttatcgtatt ggctatttca atcagctgct ctttttccat
     5581 ctcagctacc cagcaaacgc agcatttccg cttcgtcgat gacttcaatg cccagttcct
     5641 gcgctttcgc cagtttagaa cctgccgctt ctccggcaat caccagatcg gttttcttcg
     5701 acacgctgcc cgcgactttc gcgcccagtt caaccagtcg agctttagcg tcatcacgcg
     5761 acatctggct taagctgccc gtaagcacca cggttttacc agcaaacggg ctgtcaatct
     5821 cttccgcgtt gataacgatc ggcgcaggcc agtgaacacc ttccgccaac agctcgctga
     5881 tgacattgcg gttgctttct tcggcaaaga agttgtgaac gtgggatgca acgacaatgc
     5941 caacatcagg caccttttgc agctcttcaa tcgaagcggc ttccagcgct tccagcgtgc
     6001 cgaaatatgc cgccagacct gctgcggtgg cctcgccgac ttcacggatg ccaagtgcat
     6061 agaggaagcg agcaaaggtg gtttctttcg ctttttctag cgcgttaacc acgttttgtg
     6121 ccgattttgg ccccatacgc tccagtccgg tcagtttgcc tgcggtgagt ttgaacagat
     6181 ctgccggagt atggacatat tctttttcaa ccagctgatc gatgattttg tcgcccattc
     6241 cgtcaacatc catcgcacgg cgggaaacaa agtgtttcag cgactcttta cgctgcgctc
     6301 cgcaaatcag gccaccggta cagcgggcaa ccgcttcacc ttccacacgc tcaacgtcag
     6361 aaccacatac cggacaatgc gtcgggaata caacctcacg ggtatcttcc gggcgttcag
     6421 aaagcacgac gttaaccacc tgcgggatca cgtcgccagc gcggcgaatc accactttat
     6481 cgccaatgcg taaaccaaga cgttcgattt catccgcatt gtgtaaggtt gcgttactca
     6541 ccagcacgcc agcaacatgg acaggttcca gacgcgcaac aggcgtaatc gcgccagtac
     6601 gcccaacctg aaactcgacg tcacgcacaa aggtcatctg ctcctgcgcc gggaatttaa
     6661 acgctaccgc ccagcgcggg gcacgcgcga caaagccaag ctgctcctgc tgttccagtg
     6721 agttgacctt aatcaccacg ccgtcgatat caaagcccag cgtcgggcgg tcttcttcca
     6781 ctttgtgata gaacgccagc acttcttccg ccgattcaca aagcgttacc cgatcgctga
     6841 ccggcaaccc ccacttttta aattgcagta aacggccaag atgagtatcc ggcagctcgc
     6901 caccttccag aacaccaacg ccatagcaga aaaaagtgag cggtcgcttc gctgtaatac
     6961 gcggatcaag ctgacgcagt gaaccagctg ccgcattacg tgggttagca aaaactttcc
     7021 cgcccgtgcg tcgcgcatct tcgttaatct tttcgaaccc cgcctgcggc aggaacactt
     7081 caccacgcac ttccagacgc gccgggatat tctctccgtg cagcttcagc ggaatagcgc
     7141 gaatagtacg cacattagac gtgatatctt ccccggtggt gccatcgcca cgggtcgcgg
     7201 cactgactaa aacgccattt tcatacagaa tactgacggc aaggccatcc agcttcagct
     7261 cacagcacca ggtgactttc tcgttgtttt tcagacggtc ctgcacacgt ttgttgaaag
     7321 caagaaagct ttcttcatca aaaacgttat ccagtgacag cattggtact tcatggcgta
     7381 tctggctgaa agccgccagc ggcgcagcgc ctacacgttg ggtaggcgaa tcaggcgtaa
     7441 tcagttctgg atgtttggtt tccagctcgc gcagttcgcg catcagcctg tcgtattcag
     7501 cgtcgggaat ttccggcgca tccatcacat gataaagata ttcatgatgg cgaagcgtcg
     7561 ttcgcagttc tgtcagttgt tgttcgattg attccatatc gcaccatcaa tgctaaaaac
     7621 ccccgacaag cgggggttcg aagaggagtt aatttgcctt aagtgtatca ggcgttggcg
     7681 tctttgactt cgcggatgat gtcctggtac tcgcgcaatt tctgcggagt catcatacgg
     7741 cgctgatcgt caagcacgac accgcccact tcatcggcaa tatgctgcgc agattgcagc
     7801 atcagcttga agttctgcag ctcgtcaccg taagacggta cctgcataaa gatggtgaca
     7861 cccggagtag tgaaatcctt catttcagga tcaaaggttc ccggtttcac catattcgcc
     7921 aggctgaata acgccgggcc gctgccatcc gggctaagat gacgatggta aatattcata
     7981 tcgccaaaaa tgaagcccgc ttgttgaatg ctgttaagaa gcagttcacc gtttagctcg
     8041 ctaccgtgat gcgccgcgac gttcatgata atcaccgctt ctttgcgctt cggtttatcc
     8101 ataactggag ccggttccgc tacaggctca ggctgtggtg ccgctacggg ttctgcaggc
     8161 tggaaagcct gttgtgccgg ttgcggtgct gaatgcacag gctgcggcgc tggcgcgacc
     8221 tgtggcgaaa ctggctgctg caacggctgt tcaggctgcg gctgataggc aggctgctgc
     8281 accggctgcg ctggacgcgg agcatgttgc ggcggtacct gcgcttcagg cggctgctgg
     8341 accggttgac gcggctgcgc agacgcataa ggcggttggt actggtgttg cggcgacgga
     8401 cgagcagcct catgctcctg agcgttagcc ggggcatgat tcacgcggtg aacacgaacc
     8461 tcaccaacgc cctcatcatc ttcgacatcc tcgtcataag aatcgtcgtc acgttttgac
     8521 ttcattcgtt ttaatggccg atcgcggaac atagaagatc gttctttacg gctggtccag
     8581 aaaccatgta ccagtaaagc gattatggcg atcgcgccaa caatgattaa tatcagacgc
     8641 aaatcctgca tcattatatt ctctgttgtt ctaacacctt gccaccacgg caaacattta
     8701 ctcactaaga gtatttgccg attacctcaa gtgcaagtgc actattaact ttcacagcac
     8761 aaagatagat gaaatcgtgc tttttgctgt tttttcgaac atatcctaac tgtccattgc
     8821 gcaattaccc ggtaaaatac gcagaatttt cctgggattg gtcaaaagga gctcatcctg
     8881 attatggttt catcattcac atctgcccca cgcagcggtt tttactattt tgcgcaaggc
     8941 tggaagctcg tctcgcaacc tgggattcgg cgtttcgtta ttttaccgct gctggtcaat
     9001 attttgttga tggggggcgc attctggtgg ctctttacac agctcgatgt ctggatcccg
     9061 actctcatga gttacgttcc ggactggcta caatggctga gttatctgtt gtggcctctg
     9121 gctgtcatct ctgtgctgtt agtgtttggc tctttcttct ccacgattgc taactggatt
     9181 gccgctccgt ttaacggttt attggctgaa caactggaag cacgattgac tggcgctaca
     9241 ccgccagata ccgggatttt cggtatcatg aaagatgtgc cgcgaatcat gaaacgcgaa
     9301 tggcaaaaat ttgcctggta tctgccgcgc gcaattgtat tgctaattct ttacttaatc
     9361 cctggtattg ggcaaaccgt cgcgccggta ctgtggttcc tgtttagcgc ctggatgtta
     9421 gccatccagt attgcgatta ccccttcgat aaccacaaag tgccgtttaa agagatgcgc
     9481 accgccctgc gcacacgcaa aatcaccaat atgcagtttg gtgctttaac cagcctgttt
     9541 acgatgatcc cgctgcttaa tctgttcatc atgcccgttg ccgtttgtgg cgcgacggcg
     9601 atgtgggtcg attgctatcg cgataaacac gcgatgtggc ggtaacaatc taccggttat
     9661 tttgtaaacc gtttgtgtga aacaggggtg gcttatgccg ccccttattc catcttgcat
     9721 gtcattattt cccttctgta tatagatatg ctaaatcctt acttccgcat attctctgag
     9781 cgggtatgct acctgttgta tcccaatttc atacagttaa ggacaggcca tgagtaagat
     9841 ttttgaagat aactcgctga ctatcggtca cacgccgctg gttcgcctga atcgcatcgg
     9901 taacggacgc attctggcga aggtggaatc tcgtaacccc agcttcagcg ttaagtgccg
     9961 tatcggtgcc aacatgattt gggatgccga aaagcgcggc gtgctgaaac caggcgttga
    10021 actggttgaa ccgaccagcg gtaataccgg gattgcactg gcctatgtag ctgccgctcg
    10081 cggttacaaa ctcaccctga ccatgccaga aaccatgagt attgaacgcc gcaagctgct
    10141 gaaagcgtta ggtgcaaacc tggtgctgac ggaaggtgct aaaggcatga aaggcgcaat
    10201 ccaaaaagca gaagaaattg tcgccagcaa tccagagaaa tacctgctgc tgcaacaatt
    10261 cagcaatccg gcaaaccctg aaattcacga aaaaaccacc ggtccggaga tctgggaaga
    10321 taccgacggt caggttgatg tatttattgc tggcgttggg actggcggta cgctgactgg
    10381 cgtcagccgc tacattaaag gcaccaaagg caagaccgat cttatctctg tcgccgttga
    10441 gccaaccgat tctccagtta tcgcccaggc actggctggt gaagagatta aacctggccc
    10501 gcataaaatt cagggtattg gcgctggttt tatcccagct aacctcgatc tcaagctggt
    10561 cgataaagtc attggcatca ccaatgaaga agcgatttct accgcgcgtc gtctgatgga
    10621 agaagaaggt attcttgcag gtatctcttc tggagcagct gttgctgcgg cgttgaaact
    10681 acaagaagat gaaagcttta ccaacaagaa tattgtggtt attctaccgt catcgggtga
    10741 gcgttattta agcaccgcat tgtttgccga tctcttcact gagaaagaac tgcaacagta
    10801 atgccagctt gttaaaaatg cgtaaaaaag caccttttta ggtgcttttt tgtggcctgc
    10861 ttcaaacttt cgcccctcct ggcattgatt cagcctgtcg gaactggtat ttaaccagac
    10921 taattatttt gatgcgcgaa attaatcgtt acaggaaaag ccaaagctga atcgatttta
    10981 tgatttggtt caattcttcc tttagcggca taatgtttaa tgacgtacga aacgtcagcg
    11041 gtcaacaccc gccagcaatg gactgtattg cgctcttcgt gcgtcgcgtc tgttaaaaac
    11101 tggcgctaac aatacaggct aaagtcgaac cgccaggcta gactttagtt ccacaacact
    11161 aaacctataa gttggggaaa tacaatgttc cagcaagaag ttaccattac cgctccgaac
    11221 ggtctgcaca cccgccctgc tgcccagttt gtaaaagaag ctaagggctt cacttctgaa
    11281 attactgtga cttccaacgg caaaagcgcc agcgcgaaaa gcctgtttaa actgcagact
    11341 ctgggcctga ctcaaggtac cgttgtgact atctccgcag aaggcgaaga cgagcagaaa
    11401 gcggttgaac atctggttaa actgatggcg gaactcgagt aatttcccgg gttcttttaa
    11461 aaatcagtca caagtaaggt agggttatga tttcaggcat tttagcatcc ccgggtatcg
    11521 ctttcggtaa agctctgctt ctgaaagaag acgaaattgt cattgaccgg aaaaaaattt
    11581 ctgccgacca ggttgatcag gaagttgaac gttttctgag cggtcgtgcc aaggcatcag
    11641 cccagctgga aacgatcaaa acgaaagctg gtgaaacgtt cggtgaagaa aaagaagcca
    11701 tctttgaagg gcatattatg ctgctcgaag atgaggagct ggagcaggaa atcatagccc
    11761 tgattaaaga taagcacatg acagctgacg cagctgctca tgaagttatc gaaggtcagg
    11821 cttctgccct ggaagagctg gatgatgaat acctgaaaga acgtgcggct gacgtacgtg
    11881 atatcggtaa gcgcctgctg cgcaacatcc tgggcctgaa gattatcgac ctgagcgcca
    11941 ttcaggatga agtcattctg gttgccgctg acctgacgcc gtccgaaacc gcacagctga
    12001 acctgaagaa ggtgctgggt ttcatcaccg acgcgggtgg ccgtacttcc cacacctcta
    12061 tcatggcgcg ttctctggaa ctgcctgcta tcgtgggtac cggtagcgtc acctctcagg
    12121 tgaaaaatga cgactatctg attctggatg ccgtaaataa tcaggtttac gtcaatccaa
    12181 ccaacgaagt tattgataaa atgcgcgctg ttcaggagca agtggcttct gaaaaagcag
    12241 agcttgctaa actgaaagat ctgccagcta ttacgctgga cggtcaccag gtagaagtgt
    12301 gcgctaacat tggtacggtt cgtgacgttg aaggtgcaga gcgtaacggc gctgaaggcg
    12361 ttggtctgta tcgtactgag ttcctgttca tggaccgcga cgcgctgccc actgaagaag
    12421 aacagtttgc tgcttacaaa gcagtggctg aagcgtgtgg ctcgcaggcg gttatcgttc
    12481 gtaccatgga catcggcggc gacaaagagc tgccatacat gaacttcccg aaagaagaga
    12541 acccgttcct cggctggcgc gctatccgta tcgcgatgga tcgtaaagag atcctgcgcg
    12601 atcagctccg cgctatcctg cgtgcctcgg ctttcggtaa attgcgcatt atgttcccga
    12661 tgatcatctc tgttgaagaa gtgcgtgcac tgcgcaaaga gatcgaaatc tacaaacagg
    12721 aactgcgcga cgaaggtaaa gcgtttgacg agtcaattga aatcggcgta atggtggaaa
    12781 caccggctgc cgcaacaatt gcacgtcatt tagccaaaga agttgatttc tttagtatcg
    12841 gcaccaatga tttaacgcag tacactctgg cagttgaccg tggtaatgat atgatttcac
    12901 acctttacca gccaatgtca ccgtccgtgc tgaacttgat caagcaagtt attgatgctt
    12961 ctcatgctga aggcaaatgg actggcatgt gtggtgagct tgctggcgat gaacgtgcta
    13021 cacttctgtt gctggggatg ggtctggacg aattctctat gagcgccatt tctatcccgc
    13081 gcattaagaa gattatccgt aacacgaact tcgaagatgc gaaggtgtta gcagagcagg
    13141 ctcttgctca accgacaacg gacgagttaa tgacgctggt taacaagttc attgaagaaa
    13201 aaacaatctg ctaatccacg agatgcggcc caatttactg cttaggagaa gatcatgggt
    13261 ttgttcgata aactgaaatc tctggtttcc gacgacaaga aggataccgg aactattgag
    13321 atcattgctc cgctctctgg cgagatcgtc aatatcgaag acgtgccgga tgtcgttttt
    13381 gcggaaaaaa tcgttggtga tggtattgct atcaaaccaa cgggtaacaa aatggtcgcg
    13441 cctgtagacg gtaccattgg taaaatcttt gaaaccaatc acgcgttctc tatcgaatct
    13501 gatagcggcg ttgaactgtt cgtccacttc ggtatcgaca ccgttgaact gaaaggcgaa
    13561 ggcttcaagc gtattgctga agaaggtcag cgcgtgaaag ttggcgatac tgtcattgaa
    13621 tttgatctgc cgctactgga agagaaagcc aagtctaccc tgactccggt tgttatctcc
    13681 aacatggacg aaatcaaaga actgatcaaa ctgtccggta gcgtaaccgt gggtgaaacc
    13741 ccggttatcc gcatcaagaa gtaattcttg ccgcagtgaa aaatggcgcc catcggcgcc
    13801 attttttatg cttccgccag cggcggcaaa atcaattcat cgctctcatg ctgctgggtg
    13861 tagcgcatca cttccagtac gcgcaacccc gcacggtgca ccgcatcgtt taacgccttc
    13921 cctttcagca aaccactgat aagctgagca caaaacaggt cgccagtccc tttcaggtcg
    13981 gtttttaccc gtgaatggga aatgacattc acgctgtcgg cactgaccac caccacctgc
    14041 atctcctgat tttcttcatt accggaggcg ctggtaatca ccacccattt taatgtgtct
    14101 gaaagcagac tttttgcggc agcaatggca ctgtcgagat cgcggcaatt tttaccggtc
    14161 aggatttcca gctcaaagat attgggggta attccctgcg ccagcggcag taaatattgt
    14221 cgatacgcct cgggaaggtc aggtttgaca taaattccgc tatcaatatc gccaatcacc
    14281 ggatcgacca tgatcaatag gtcaggatgg tctttgcgta gcgcagtcag ccactcggca
    14341 aggattttga tttgcgatgc cgttcccata tagcccgtgg ttacagcgcg cagctggcgc
    14401 agcgcatcac gctcctgaag cgcacgcaaa taaccgctaa accattcatc cggaatcgca
    14461 ccaccgtaga aagtgtcata atgcggcgta ttgctcagca ataccgtcgg cacggcaaaa
    14521 acattcagac cgttctgttt gatagcaggc acggcaatgc tgttgcccac gctgccgtaa
    14581 accacctgcg actgcacggc gacgatatcc gcctgcagcg ccctgctctt atcgttaaac
    14641 aacaacaaac tactcattta attttttctc cttgccgatg atcctcatcg tattccaacc
    14701 gaaactttac ctgattgtgg cagtcaaatc ggctatcaca aaacaaggat aaggtaattc
    14761 aatgaagaaa atcatttgtc tggtcattac actactaatg acactccccg cttacgcgaa
    14821 gttaactgcc catgaagaag cccgcatcaa cgccatgctg gagggattag cacagaaaaa
    14881 ggatttgata tttgtgcgca acggtgatga acatacctgc gatgaagcgg tttctcatct
    14941 gcgtctgaag ctgggcaata cccgtaaccg cattgacact gccgagcagt ttattgataa
    15001 ggttgcttcg tcgtcatcga ttaccgggaa gccgtatatt gtgaagatcc ccggtaagag
    15061 cgatgagaac gcacagcctt ttttacatgc gctaattgcg cagacggata aaacggtgcc
    15121 tgcgcaataa tcttaaatcc ccgccccctg gctaaaatgc tcttccccaa acaccccggt
    15181 ggaaaggtag cgatcgccac gatcgcagat gatcgctacc accaccgcac cagggttagc
    15241 ttttgccacc cgcagtgctc cggcaaccgc gccgccggag ctgacgccac agaatattcc
    15301 ttcccgcact gccagttcgc gcatggtgtt ttctgcatcg cgctgatgaa tatccagcac
    15361 ctcatccacc agagaagcgt tgaaaatccc cggcagatat tccgcaggcc agcggcgaat
    15421 gccgggaata ctgctgcctt cttccggctg caggccaaca atcgttactg gtttggattg
    15481 ttcgcgcata aagcgtgaaa cgccggtgat agtgccggtt gttcccatgc tggagacaaa
    15541 atgggtaatg cgcccgccgg tttgctgcca gatttccggc ccggtagtgg tgtaatgggc
    15601 gtaagggtta tcgggattat taaactgatc gagcagcttt ccttcgccac gattcgccat
    15661 ctccagcgcc agatcgcgcg caccttccat gccctgctct ttggtgacaa gaatcagttc
    15721 cgcaccataa gcacgcatcg ccgcacggcg ttcctggctc atgttgtcgg gcatcagcaa
    15781 tttcatgcga tagcctttca gcgcggcaat cattgccagc gcaatgccgg tgttaccact
    15841 ggtggcttcg attaagacat cacccggttt aatttccccg cgcttttccg cctcgacgat
    15901 catcgaaagt gccgcacgat ctttcaccga acctgccggg ttattgcctt ccagttttaa
    15961 ccacacttca ctgccgttat ccggccccat tcgctgcaac ttcaccagag gcgtattgcc
    16021 tattgtttgt tctaatgtac tcacgatctc tatccatacg tggtgttgcc tgatgcgacg
    16081 cttgcgcgtc ttatcaggtc tacaggttac aaaccttgcc attaaaaagc ccggatcgcg
    16141 ggaagcgcct ccgggcgttt aacattcact caacctatca ggcgctttgt gcgagagcaa
    16201 gttcctcatc tcgggtttcg atacgctcgt cgccgttata cagccgcgca tgttgcagac
    16261 caacgaataa acgctcgcca cgctgcgggg catcgtcgcc atgcatcacg accgtcagcg
    16321 gttcgttgta ccaccccagc ggctgcacca ctaattgggt gtagtgacct ttcgggctgg
    16381 cttccagtac ctgtaccggc agcggcgaat cgagactggt acggcggctg atatccactt
    16441 cccacgggcg caggaagaga tccaccggac cctgatacgc aggtgtgtag cccagcggcc
    16501 agcgatgcgc gccaacatgg aactgcccgc cgcgaatggt tccctgcagg cggttcactt
    16561 cgcccataaa ttcgagcaca aaacgggtcg ctggttcgcg ccatacctgc tccggcgcat
    16621 cagcctgttc aatattgccc tggctcatca ccactacacg atcagctact tccgtcgctt
    16681 cttcctggtc gtgggtcacg aacacgctgg tgaatttcag ctcttcatgc agttgacgca
    16741 gccagcgacg cagctcttta cgcacctgcg catccagcgc gccaaacggt tcatcaagca
    16801 gcagaatttg cggttctaca gccagcgcgc gcgccagcgc cacgcgctgt ttctggccgc
    16861 cggaaagctg tgccggataa cgatctgcca gatgagcaag ctgcaccatc tccagcaatt
    16921 ttgtcacttt cgctttgatg gctgcggcat tcgggcgctc gcgacgcggc agcaccgtca
    16981 ggccaaaagc gatattgtcg aacaccgtca tatgacggaa cagcgcgtaa tgctggaaca
    17041 cgaaaccgac cttgcgatcg cgcgcgtgca ggcggctgac gtcggtgccg tggaagcgaa
    17101 tatgcccgct ggtttgatgc tccagcccgg cgataatgcg cagcagcgtg gttttcccgg
    17161 aaccggacgg ccccagcaac gcgaccatct gacctgaagg aatatccagt gagatatcgt
    17221 tcagcacctg ggtgcgacca aacgacttct taatattggc aatctcaatg ctcatgatgt
    17281 tcctcctgct gtgcgcgttt ttcctgattc tccaggcgcc actgcaacat actttttaaa
    17341 aacagggtga taatcgccat cagcgttaac agtgccgcag cggtaaagga gccgacggtg
    17401 ttgtagtcct gctccagcaa ttcaatctgt aacggcagcg aaagtgtttc gccgcgaatc
    17461 gagccggaaa ccaccgacac cgcgccaaac tcgccaatcg cgcgggcgtt ggtcagcact
    17521 acgccataaa gcagcgccca gcggatgttc ggcaatgtga cacgacggaa catctgccag
    17581 ccagacgcgc caagcaaaat cgccgcctcg tcttcctgac tgccctggct taacatcacc
    17641 ggcaccagtt cgcgcaccac aaacggacac gtcacgaaga tggtgaccag caccattccc
    17701 ggccaggaga acataatttg cagattatgt tcatccagcc aaccgcccag cgggccgtta
    17761 gagccgtaga acagcagata caccagaccg gcaaccaccg gcgatacggc aaacggaatg
    17821 tccagtagcg tcagcagtaa ctggcgtcca gggaagttaa agcgcgtcac cagccaggcc
    17881 agcagaatgc cgaacaccag gtttaccggt acggcaatca gcgcgatcat caccgtcagc
    17941 cagatggcgt gcagcatgtc cggatcggcc agattctgta aaaccggcat cagccccttg
    18001 ctgaatgcct gcacgaagat gtaaatcatc ggcaccagca ggatgaacgc tgaaaccagc
    18061 atcccgatgc caattagaaa ccatttgccc cagttaatcg ggcgcgcgtc ataacgcttc
    18121 aattgggtaa cttccgccat taatgaccta ccacacgccg accaaagcga ctttgcagag
    18181 tgttaattga gaacagcagc agcagggatg ccgcgaggat caccgaagca atcgcgctcg
    18241 ccgccgggta atcaaactcc tgcaagcgca caaaaatcat cagcgacgtc acttccgtct
    18301 tccaggcgat gttcccggcg ataaatatca ccgcgccaaa ctcaccaaga ctacgggtaa
    18361 acgacaacgc cacgcccgcc accagcgctg gagaaagttc cggcagcacc actttgcaga
    18421 aactctgcca gcgcgttgca ccaagcgttt ccgccgcttc ttcatattcc gggcctaact
    18481 cttccagcac cggctgcacg gtacgcacca caaacggaat gctggtaaag gccatcgcca
    18541 ccgcaatacc aagccaggta taggtgactt tgatatcaaa cttcgccagc cattcaccgt
    18601 aaaaaccgtt tacggaaaag agcgaggcca gcgttaaacc ggcgactgcc gttggcagcg
    18661 caaagggtaa atccatcagc gcatcaagca gcgtgcggcc cggaaagcga taacgggtta
    18721 agatccacgc catcagcagg ccaaaaacgc cgttaaaaat cgatgccaca aacgctgaca
    18781 gcagcgttac tttgtaggcc gctactacct gcgggttggt gatcacctcc cagtactgcg
    18841 cccagctcat ctgagccagc tgcatcacca gcgcggagag cggcagcagc aaaatcaggc
    18901 acacaaacag cagactggtg ccgaggctta aggtaaagcc cggcagcacg cgtcttgatg
    18961 aaactgcaaa cattaattac gccccgccgc taacagcttg tctaactcgc cgccgctggt
    19021 gaagtgggtt ttcatcactt ccggccagga gccaaatttg tcttccacgc ggaacagctc
    19081 ggtctgcggg aatttgtttt tcagtttgtc catgacttcc gggttattca cacgatagta
    19141 atagtcggtg atgatggtct gcgcctgtgg gctgtagagc cagttcaggt aggcttttgc
    19201 ggctttttcc gtaccgttgg cctgcacatt tttatcgacc cacgccaccg ggaattccgc
    19261 cagaatattg gttttcggga tcaccacttc aaagccctgt gcttcatact gtttacggat
    19321 gttgttcact tccgattcaa agctaatcag cacatcgccc aggccacgct cggcaaaagt
    19381 ggtggttgcg ccacgaccgc cggtatcgaa cacttcaacg tttttcagga actgggtcat
    19441 aaactgttcg gttttggctt tatcgccacc gtcagcttta tccgctgcgc cccatgccgc
    19501 cagataggta taacgcgcgt tacccgacgt tttcgggttc gggaaaatca gcttcacgtc
    19561 ggagcgcacc aggtcgttcc aatcgtggat attcttcggg ttacccttac gcaccaggaa
    19621 gcccatggtg gagtagaacg gcgagctatt attcggcagg cgcgactgcc agtcggccgg
    19681 gatcagcttg cctttatcgt gcaggatttg tacgtcggtc acctggttat aagtgacaac
    19741 gtcggctttt aagccctgta aaatcgccag cgcctgtttt gatgacccgg catgagattg
    19801 ttttatcgtc agtttgtcgc cgccgttatc ttttgcccat tgttgctcaa acggcggatt
    19861 cagggcggca aacagctcgc gggagacgtc ataagaactg ttcagcagtt ccgttgcctg
    19921 tacatggccc gccagcagca gagaagcgac cagcgcgagt gagttctttt tcagtaagtt
    19981 aacggccatt gcgcaccctt ataaatttaa tgactttcta atagccatca tatttataac
    20041 ggacgtgaaa ggagtaacgg ttttatatac cgtttggtga tttggaagtt gaaaagggaa
    20101 taagaaccgg gtttaaaacg aaatcctccc cacaaatgca gggaggaaac agaggtgaat
    20161 cagataccga cgctaaccgt ctccggcagt gtgctgccgc catcaatcac attctgtgta
    20221 ccggttaaat agctggattc atccgatgcg aggaaggccg ccagttcgcc gacttccagc
    20281 ggatcggcga ggcgacgcat cgggattgct ttcgccattt cagtcagcac cgactctgga
    20341 tcttccgggt tcgactggcg ggcaatgctt tccgccattg gtgtgcgcac gtatcccggg
    20401 caaatggcgt taacgcgaat accagactgc gcgtactcca ccgccagcga ttttgtcagg
    20461 ccaacaatcg ccgctttcgt taaggcgtac gccgtttcgc caggatcggc caccatatcg
    20521 ccagtgaccg aagacatcat cacaatgcga ccatctttgc gggcaatcat ctccggcagc
    20581 accgccttcg tgacgttcca tacgccttta atattgatgt caatatggaa atcgcgatcg
    20641 tcatcgctca tatcgaggaa actgcccaga cgacaaacgc ctgcgttatt caccaggata
    20701 tcaatgcgcc cttctttttc cttcgcgcgt ttgatagctg cggctaccga cgccgggtca
    20761 cgcacatcgg cgacaaccgc cgtacagcga tgaccacgac cacacagttc gtccgccagc
    20821 ttttcgatct caggggagat atccagcaag attaggtttg cgccatgacg cgcaaaagtt
    20881 ctggcaattc cttcgccaat tccctgcaat gcgcccgtaa tcagtgctgt cttgcccgtg
    20941 agtttaccca ttttaatgct ctccttgtta atacagagcg ttacactcca ttccctctga
    21001 aaatacagga taaacaaaat attgctgcgg atcagctcac taattcagga aaatctcaat
    21061 ccagtcgctg ctttatgcat catttcaggc gttgtgttag ctcactgctg cgctgaatca
    21121 ttttcaatga ctccacatca ttgagttgca caagaccaac aaacagtaaa tcggtaacag
    21181 aattttgcgc cgtgcgggta gacatcgacg agctacgcca ttcggtttca ccggaaacgg
    21241 tatcgagcgt gaaatgcgcc agccgccgca gcggagaatc agccagagaa gtaatggcaa
    21301 tcaccgttgc cccctgcttt cgtgccgctt ctgcgcataa gacaatctct tttttactgc
    21361 cgctgtaaga gatagcgatt tgtacatcgc cttttttcaa cgcctgggaa acggtggctt
    21421 gtacgtgcgt gtcggcttcg caggccacgc gatagccaat tttcatcagt ttgaacgaca
    21481 gatcgcgccc caccagcgcc gatccgccca ggcccgttat ctggataaac ggtgctttac
    21541 tgatgacctc aatgattttt tgcagccgcg cgtagtcgaa caacgaacag gtttgctcca
    21601 gcgccagctc tttttcgcga ttcaatttgc gggcaatcac ttccagcgaa tcatcactgg
    21661 taatcgaact gtgcaggtgc agcgccgtgg cattcgtttt ctcccggctg gcactgtatt
    21721 cgcctattaa cgccatacgc aattcagtaa atccttgcgc gccgagtttt tgggcaaatt
    21781 tcactatgct cgactggcta atgccgagct gtttcgccat ctgccgtgaa gaaacggatt
    21841 gcagctcact cacgttggcc tgcagaaagt cagcgatttt ttgctcgttt tcggtgaatt
    21901 cactccccgc attgcttatt ttcgtcaggt acaacatctt ccactctcct gggctggcag
    21961 cataaattcc gcttaagttc ttatgacgct cttcacactc tgcgagtgta gactcaatga
    22021 ttcctttaag tctgtcaatt cgggatatat gattccatac atctgcaatt atttgatcgt
    22081 aaatagtaag gtcaccaccg atgcaactag aaaagatgat tactgaaggc tcgaacaccg
    22141 cctcggctga aattgaccgc gtatcgacgc tggaaatgtg ccggattatc aacgatgaag
    22201 ataaaaccgt accgcttgcc gttgagcgcg tcctgccgga tatcgctgcg gcgatcgatg
    22261 ttatccacgc ccaggtcagc ggcggcggtc gtctgattta tctcggtgcg ggaacatccg
    22321 gtcgtctggg gattctggat gccagcgaat gtccgcccac ctacggcgtg aaaccgggtc
    22381 tggtggttgg tttgattgct ggaggcgaat atgccattca gcacgcggtg gaaggcgctg
    22441 aagatagccg ggaaggtggt gttaacgatc tgaaaaatat taatttaacg gcacaggatg
    22501 tggtggttgg cattgctgcc agcggtcgca cgccgtatgt gattgccgga ctggaatacg
    22561 cacgccagct cggctgccgc acagtgggaa tttcctgtaa tccggggagt gccgtttcca
    22621 ccaccgccga gtttgccatt acaccgattg taggtgccga agtggtcact ggttcttcgc
    22681 gaatgaaagc aggcacagcg cagaaactgg tgctcaatat gctttccacc gggctgatga
    22741 ttaaatctgg caaagtgttc ggcaacctga tggtcgatgt ggtcgccacc aacgaaaaac
    22801 tgcatgtgcg ccaggtcaat atcgttaaaa acgccaccgg atgtaatgcc gaacaagcgg
    22861 aagcggcgtt aattgcctgc gagcgcaact gtaaaacggc cattgtgatg gtgctgaaaa
    22921 atctcgatgc cgcagaagct aaaaaacgcc tggatcaaca cggcggcttt attcgtcagg
    22981 ttttagacaa ggaataaccc atggccaaag agatcagcag tgaacttctg aacaccattc
    23041 ttacccgtgt cggcggaccg ggaaatatcg ccagttgtgg taactgtatg acgcgcctgc
    23101 gtctgggtgt acatgacagt tcactggttg atcccaatat caaaacgctg gaaggcgtga
    23161 agggcgtcat tttgaccagc gaccaggtgc aggtcgtttt tgggcctggt aaagcgcatc
    23221 gtgccgcaaa agcgatgagc gagctgctgg gcgaggcacc ggtacaggat gccgcagaaa
    23281 tcgccgccca gaacaaacgt cagttaaaag ccaaacaaac ctccggcgtg caacaatttc
    23341 tcgccaaatt cgccaccatc ttcacgccgc tgatccccgg ttttattgcc gccggtctgc
    23401 tgctggggat agcgacgtta attgccacgg tgatgcacgt tccggcagat gctcagggca
    23461 cgctacccga cgcgctgaat tttatgaagg tgttcagcaa aggtttgttc actttcctgg
    23521 tgattctggt gggctataac gccgcccagg catttggcgg cacgggcgtc aatggcgcaa
    23581 ttattgccgc actctttttg ctcggttata accccgctgc aaccaccggt tactacgccg
    23641 gttttcacga tttctttggc ctgcccatcg atccgcgcgg caatattatc ggcgtgctga
    23701 ttgccgcgtg ggcctgcgcc cgcattgaag gcatggtgcg ccgctttatg ccggacgatc
    23761 tcgacatgct gctgacctcg ttaatcaccc tgctgatcac cgctacgctt gcgtacctga
    23821 tcattatgcc gctgggtggc tggttattcg aaggtatgtc atggctgttt atgcacctga
    23881 acagtaatcc gttcggttgt gcggttttag ccgggctgtt cctgatcgcc gtggtatttg
    23941 gcgtgcatca gggctttatt cctgtttacc tcgcgttaat ggacagccag ggattcaaca
    24001 gcttatttcc catcctttca atggcaggcg cgggccaggt gggcgcggcg ctggcactct
    24061 actggcgggc gcaaccgcac agtgcattac gcagtcaggt acgcggggcg attattcccg
    24121 gtctgctggg cgttggtgaa ccgttgattt acggcgtcac cctgccccgc atgaaaccgt
    24181 ttattaccgc ctgtttaggc ggcgcagcgg gcggtttgtt tatcggcctg atagcctggt
    24241 ggggtctgcc gatgggctta aacagcgcct ttgggccgtc tggtctggtg gcgctgccgc
    24301 tgatgacttc cgcacaaggc atccttccgg caatggcggt ttatgctggc gggattctgg
    24361 tggcatgggt ttgcgggttt attttcacca cgctctttgg ctgccgtaac gtcaatctgg
    24421 actgatatca tgaaacggac aatgctctat ctttctctgc tggctgttag ttgtagcgtc
    24481 agcgccgcaa aataccctgt tctgacagaa agttcgccag agaaagcagg gttcaacgtc
    24541 gaacggctta accagatgga tcgctggatt agccagcaag tggacgccgg ttatcccggc
    24601 gtaaacctgc tgatcattaa agataatcag attgtgtatc gcaaagcctg gggagcggcg
    24661 aaaaagtacg atggcagcgt gttgatggaa cagcctgtca aagccaccac cgggacgctg
    24721 tatgacctgg cctcaaacac caaaatgtac gccacgaact tcgccctgca aaagctgatg
    24781 agcgaaggca aactgcatcc tgacgatcgg attgcgaaat atattccggg gtttgccgat
    24841 agtccaaatg acaccatcaa aggcaaaaac acgctgcgga tttctgacct gctgcatcac
    24901 agcggcggtt tcccggcgga cccacaatat ccgaataaag cggtcgcggg cgcgttatat
    24961 tcccaggata aaggccagac gctggagatg atcaagcgca cgccgctgga gtatcagccc
    25021 ggcagcaagc atatttacag tgatgtcgat tatatgctgc ttggatttat cgtcgagtcg
    25081 gtcaccggtc agccgcttga ccgctatgtt gaagagtcga tttatcgccc gctcggcctg
    25141 acacatacgg tatttaaccc gctgctgaaa ggctttaaac cgcagcaaat tgccgccacg
    25201 gaattgaacg gcaatacccg cgatggcgtg atccatttcc cgaatatccg cacctccact
    25261 ctctggggtc aggtgcacga tgaaaaagcc ttttattcga tgggcggcgt ttccgggcac
    25321 gcaggtttgt tttccaatac cggcgatatt gcggtgttaa tgcaaacgat gctgaacggc
    25381 gggggctatg gcgatgtgca gctgttcagt gcggaaacgg tgaagatgtt caccaccagc
    25441 tctaaggaag atgccacttt tggcctcggc tggcgcgtga atggtaatgc caccatgacg
    25501 ccgacgtttg gcacgctggc aagcccgcaa acctacggtc acactggctg gaccggaacg
    25561 gtgaccgtta tcgatccggt gaatcatatg gcgattgtga tgttaagcaa caagccacat
    25621 tcgccggttg ccgatccgca aaagaatccc aatatgttcg aaagcggtca gctgccgatt
    25681 gcaacgtatg gttgggtagt tgatcaggtg tatgcggcgt taaagcaaaa gtaataaaaa
    25741 ggccagtcag taatgttact gcctggcctt taatcaatga atcagaaacg cttacagcgc
    25801 catcaacttg tccagcgacg gtgcgaaata atagccgccg gttaccggtt tggtgaaacg
    25861 caacatcgca tcacgcttac catcggtatc gccaaacatg ctcagcagtt gctgctcaat
    25921 gttatgcaga cgcgcgcagt aggcgcagaa gtacagaccg tgagtgccac tggcagtgcc
    25981 gtacggcagg ctctggcgaa caatcttcag ccctttgcca tcttctttca gatcaacgcg
    26041 ggtgaggtga gaggtttccg gacgttcgtc gccgtcgatc tcttcgttgg cctctttggt
    26101 gcgcccgatc atcatctcct gatcgtgaac gctcatccgg ttgagctgct tcaggttgtg
    26161 ttcccaacgc tggacaaaca catagctgcc gcccgcatcc acgccgtctt tgataaccgc
    26221 cacttcgcga cgcgtctctt cacccgccgg gttttccgta ccgtcaacaa agccgctcag
    26281 gtcacgctct tcaacccaac ggaagccgtg gatctcttct ttcacttcaa tgcagtcacc
    26341 aaaggcttcc atcgccgcct gggcgacaga gaagtttacg tcgtgacgca gagaaagaat
    26401 gtggatcaac acatcgaact gggtggtcgg cgcaaggcct ttaccgtagc ccggaaaatc
    26461 tttcagctct tctgccccaa cgccgccgct cagagcgcgc caggtgttgt taccaaaggc
    26521 aaccaccgca ccaagatgcg cgtccgggaa tttcgcttca aaagttgcca gtttgtcggc
    26581 aaatgtttta ctggccgcac gcagggcgtc aacttcccct ttcacgttgg cttcgatcca
    26641 aatcgccgcg cggcaatgtt ctggcaaaat gccactctga acctgagaca ttgttcctcc
    26701 tgaaaataat aatgccacgc aatccgtggc attgttgagc gctattttac gtgttttttc
    26761 gcggcggcgg tttgttcaga cgcaaattaa cgccgccaga taattttact gactttccag
    26821 tttttcaggg tatcgtccga aggcattaac ccttcaggac cactccattc cccgctgaac
    26881 tggtagctaa tatgttgact gccttcggct ttacattcga cggcacgatt atcatcacca
    26941 tcagcttttt ggcaattgcc aaatgctttg ctgtaaaggt cgctaaacgg tgtaccgatt
    27001 ttaacaccgg tgtcagcagg aatatcgcta tccagcacgt caatgcgact gatcgtgcct
    27061 tgatcgccgt taatcaccat cgccacgttg tcgcccttca tcacttcaaa aaagcgcacc
    27121 acgttgccgt tcgcggtttt cattccgctg cgcaggcgat aatcgccatc aagcgcatcg
    27181 gcaatggctt gttcttgcag tggtgtggac gccgttaatt caccaacgcc ctgctcgctc
    27241 actttggtgg acgacccaaa ccagttccac gggttagcgg cagaccagtt aactgacgac
    27301 atcgtggaac agccggtcag catcaatggc atagcgcata acattaaacg cagcgatttc
    27361 atgtcacttc ctttggttat tcaataacgt tgcttggagt gcaaattcac caaaaagtgc
    27421 cgttattctt ttacttcatt aaaacaagcg cgtaaacgtc gattggtcag cagccagact
    27481 aacgccacaa tatccgccac taccagcgcc agaccgatac cgctaacgga ttcaccgttc
    27541 agccacagcc acggttgcca gcaaagtaaa accacctgcg ccaacagcaa cagaaaatag
    27601 agtacacgcc aggtgcgagg aaatgtagcc cgccgaccgc tcaacaaaaa cgccagcacc
    27661 gccggaatgc ctggaatcag ccccagccag aaattatcgt gatcgggata aaacagattt
    27721 agcagcgcag taccctgctc gcgcgacgca ccggcaatga caaacagcac ccaggttcgc
    27781 gcctgaagca atagcacaag ccagaagagc aagggtaaac gcaggcgtcc gtgcgcatca
    27841 taatggacag gatggaactc agtactcttc atcttcaatc aaacgcttac ccagactcag
    27901 cacgtcggcg tgttcatatc ccaggcgttc atacattccg agcaccatgt cgttatcttc
    27961 cggcacattg atctgaattt tcgggcagcc acgagcaatc agctttttct ccagccgatt
    28021 aagcaacgca ttggcaatcc cacgcccacg aaactctgga tgcacgccaa gataataagc
    28081 agacccgcga tgcccgtcat aaccgcccat caccgttccg accacttcac cgtttacctc
    28141 agcgaccaaa aacaaactga cgtcatggtt catcttacgc tcgatgtcca tttccggatc
    28201 gttccacgga cgcagcaagt cgcaacgctc ccaaagggtg atgacctctt cgaaatcttc
    28261 ctggcgaaat acgcgtatct ccatggtatt cgttaccttt ttgcgggtta aaaggctgat
    28321 tatggcgtga acggtcgaat tagccaatat ctgacgaaaa tcggttgaaa aagtggcata
    28381 atggggagtt gtcaactatt gaaatgaaaa gtaaaacaat tctcaacatc aaaccgtcgt
    28441 aacggattac gcgatacgat ataacatctg gaactttatt attacaactc aggccgtatg
    28501 agcactttta aaccactaaa aacactcact tcgcgccgcc aggtgctgaa agccggtttg
    28561 gctgccctga cgttgtcagg aatgtcgcaa gccatcgcca aagacgaacc tttaaaaacc
    28621 agcaacggac acagcaagcc gaaagccaaa aaatctggcg gcaaacgtgt cgttgttctc
    28681 gatccaggcc acggcggaat tgataccgga gcgatcggac gcaacggttc gaaagaaaaa
    28741 catgtggtgc tggcgattgc taaaaacgtc cgttccattt tgcgtaatca tgggattgat
    28801 gcgcgtttaa cgcgttctgg cgatacgttt atcccacttt acgatcgcgt tgaaatcgcc
    28861 cataaacatg gcgcagatct gtttatgtca attcatgccg atggctttac caacccgaaa
    28921 gctgccggtg cttcggtatt tgccctctct aaccgtgggg caagtagcgc aatggcgaaa
    28981 tacctgtctg aacgcgaaaa ccgcgccgat gaagttgccg gtaaaaaggc gactgacaag
    29041 gatcacctat tgcagcaagt gctgtttgat ctggtgcaaa ccgatactat taaaaacagc
    29101 ctgacgctcg gctcgcatat tctgaaaaag attaagccgg tgcataaact gcacagccgc
    29161 aacaccgaac aagccgcgtt tgtggtgttg aaatcgccgt cggttccttc ggtgctggtg
    29221 gaaacctcgt ttatcaccaa tccggaagaa gagcggctgt taggcacggc ggcgtttcgt
    29281 cagaaaatcg ccacagcgat tgctgaaggc gtgatcagtt atttccactg gttcgacaac
    29341 cagaaagcac attcgaaaaa gcgataagtt atgaaacccg acgcacacca ggttaaacag
    29401 tttctgctca accttcagga tacgatttgt cagcagctga ccgccgtcga tggcgcagaa
    29461 tttgtcgaag atagttggca gcgcgaagct ggcggcggcg ggcgtagtcg ggtgttgcgt
    29521 aatggtggtg ttttcgaaca ggcaggcgtc aacttttctc acgtccacgg cgaggcgatg
    29581 ccagcttccg ccaccgctca tcgcccggaa cttgccgggc gcagtttcga ggcgatgggc
    29641 gtttcactgg tagtgcatcc gcataacccg tatgttccca ccagccacgc gaatgtgcgt
    29701 ttttttattg ccgaaaaacc gggtgcagag cccgtctggt ggtttggcgg cggcttcgat
    29761 ttaacccctt tctatggttt tgaagaagac gccattcact ggcaccgcac cgcccgtgac
    29821 ctgtgcctgc catttggtga agacgtttat ccccgttaca aaaagtggtg cgacgattac
    29881 ttctacctca aacatcgcaa cgaacagcgc ggtattggcg ggctgttctt tgatgatctg
    29941 aacacgccag atttcgacca ctgttttgcc tttatgcagg cggtaggcaa aggctacacc
    30001 gacgcttatt taccaattgt agagcgacgt aaagcgatgg cctacggcga gcgcgagcgc
    30061 aattttcagc tctaccgtcg cggtcgttat gtcgagttca atctggtctg ggatcgcggc
    30121 actctgtttg gcctgcaaac tggcgggcgc accgagtcga tcctgatgtc aatgccgcca
    30181 ctggtacgct gggagtatga ttatcagcca aaagatggca gcccagaagc ggcgttaagt
    30241 gagtttatta aggtcaggga ttgggtgtaa ctccctcacc cccattcccg catccgctga
    30301 tgcaacgtca acgacggctt ctcggcaaac agctgctggt aatccgtggc aaattgcccc
    30361 agatgccaga atccccactg catggcggcg tcttttaccg ttgtgctttg cgaccacggg
    30421 cttatcagtt cgcggcgtac ggcgttcagg cgaatgcgtt tcagccacgc gtttgggcca
    30481 atgcctaaaa tagcgtgaaa cgcgttttgt agcgtgcggc gactgacatg cagttgatta
    30541 cacaagtcca gcaccgtcac cggctcagac atattttcca gcacatattc acgggcgcgg
    30601 gaaagtaatc tacggtaact ctgatgactg atgctttccg ccgtcaccat tggttgcgcg
    30661 tcttccagca tcgcccccat cgccattagc aaattatccc ccagcacttt tcgcactgca
    30721 ggctgatgga gattttccgg gttctcgcaa aacgtcgcca gcgcctgttg gacaaagccc
    30781 cacagcgcgg ctttatgctg ctctttcact tccagcgccg actggctacg cagcatatgt
    30841 aatacccgat ccgggttatg caaaaagtta gcctgccggg tgatgacatc ttcagaaagc
    30901 accacgccca ggatcgtgta atcatccggc gtgctcagtt caaattcagt gccaccaggg
    30961 cgggtggcga tttccgcgct tcccagacat tgcgaaccga taaatccctg ctcaccgcgc
    31021 gtcgccggaa tgccaaacca gaacgagttc ggccagacca ggcacgactg acgcagcgcc
    31081 agaccggtgt attcacgaaa aacctgaata tcatcgagta gaatttccgt aaattcacca
    31141 tgaaacttgc ccggatgcag ctgatcgtaa atctgctgcc aggcggtaat cgttaaagca
    31201 tgttcataga catccgttgt ctgtcgttga tgaacattat ccacttcgac cttcggcgtg
    31261 agcttcaggt tttcgggtaa gggttcatga taaagatggt gcaaattggc tgtacgggtc
    31321 tttttcatga tgttaatgcc gggtgttgta ggacacccgg cacctccgac aggttaatgg
    31381 ggcttgagac gataacgact actgcgttta cgtaacgtcc cggcagaaaa gagctgttcg
    31441 agcgcatttc tggctttttc cagcggccag ccaaagtggg cagccacttc tcctgccgtc
    31501 attccctgac gtactgatgt taacagcgcc aacaactcgt cagcagattc cgcgacgata
    31561 actggcgcgt cgggttccct tacgggttgc ttcggctggc ggttaaagcc agtaaccagc
    31621 cactgggtgt catcgtctgg ccgaccgatc tccttgcggc tgatgacacg cccggtacgc
    31681 atcgcggcag cacaacctgc gtccagcgcc gcacgacacg ccgccagatc gccttccacc
    31741 accagcgtta agcgaccagg gtcaagcact tcgtgactga gcagacgaac gttagctgct
    31801 ttgagcatgg catccgctgc atctatcgcg gcgaccatgc cgtccacttc cagcaatccc
    31861 agtgcattga tcatcggcaa cctccgttac gcacgctgga ttggattacg cgcgatttcc
    31921 agcactgcat cggtaaaggc gttacaggct gctttacacg cggcctggct accggttaaa
    31981 aatgctgccg agtagttggt ttcagacggt ggcgggacat aggtcaccag ctgtacgtcg
    32041 gcagatttca acgctgcatc aatgccatag gtcgcttcca gcggcggtgc caccagatac
    32101 gccatcggat cgccaagcgt gatcccggcg gttgatgaga gataagaacc ggtacgcgaa
    32161 accacatgcg ccaggaaggc ggtgttttct gcgtcattgg cccactggaa tgctgcacca
    32221 ttttcaatat gtgcaaccat cgcatccaga ccggcacgca cttccgccgg gtttggccca
    32281 ccaagcataa tcagcacttc accggcagtc ggtgacgggc cgtgtgcagc gcccgcatac
    32341 agcgagcgac catataccac ttccaccatc gcctgcttgg tcgcttcatc agcggcaatg
    32401 taggtgacgt catcggaatc agcagaaatc agcccgaggc tacgaatatg cggcggcaat
    32461 tttagttcac gcgcaaattc agcgttaacg gaggcaatca cccgcatggc ggttaccgac
    32521 ggtcgaatca aatctaaagc tggcatgatg cctccttatc gggtcatgtt gatgccggat
    32581 gctttctgct ccagcatacg tttcgccaaa tccacaatga cggctgcggc ttcaaccggc
    32641 ggcgtgccgc cctggtgaat gttagagata caggtgcgat cggcctcaac ggtggtcgcc
    32701 atacgcggcg agtaaacggc gtagcaggag aggctttctg actgccctaa ccccggacgt
    32761 tcccccacca gcaggatcac cactttcgcg ccgaggatct caccgatctg atcttcaatc
    32821 ttcacgcgac cataacgaac aaagaatggc gtgccgactt tcagcccggc ctgtttcagg
    32881 cccgccatca gcggcggcag gatctcttcg tagttcacgg tgatggcatc agttgacagg
    32941 ccatcagaaa taacgacctg tacgtccgga ttagcaacac actgtgcttt cagcgcttca
    33001 acagcttctg cacacaggcg gcggcccata tccgggcgag tcaggtagag atttttgtcg
    33061 ctaatttcag agcgtacttc cagcaagccc tgcgctttca cccactcttc cggcacttct
    33121 ttcagtacgg tatctttcga acgagagtga tcggccagga aacgcagcag cgcctgggta
    33181 cgtggacgcg gaccggcacg accggtacaa acgcgggcca cggtgctgcg gcgcagttct
    33241 gttaatacgt ctgcgcgatg cgggttttca acaccaatcc acgcttttgc ttcagcggaa
    33301 cctaaatcca gcgcacagct ttccgaggtc accggtgccg cacagttggt ggtggcgcac
    33361 tttgcttctg acggcgcggg ggccgtttgt cccattgacg ccatcacgct gcgtacaatt
    33421 tcttcaatct gtttttgatc catgatgtgt tatctccgcg tcatcagaag aacagtgacg
    33481 gatcgcccgc ccgtttggtc aggcgaccgt ttgccataat gcccatgctt tccagccagc
    33541 gttcaaactc cggtgacgga cgcaggttga gtaactgacg cacagtggcg gtatcgtgga
    33601 atgcggtggt ctggtagttg agcatgatgt catcacccag cggcatcccc atgatgtagt
    33661 tgcagcctgc ggtggcgagc aggatcatca ggttttcgtt gaggttctgg tcagcgtcag
    33721 cgtggttggt gtaacagcag tcacagccca tagagatgcc gctcagcttg cccataaagt
    33781 gatcttctaa gccagcacgg ataatctggc ggtcgttgta gagatactcc ggcccaataa
    33841 agccgaccac ggtgttgacg ataaacggat cgtaatgacg cgccagccca tagttacgtg
    33901 cttccatcgt cacctggtca gcgccgaagt tagcgccagc ggatagcgca gagccttgtc
    33961 cggtttcgaa gtagaggcag ttttccccgg cgatacggtt gaactctgca cccactgcgc
    34021 gcgcttcgtc gagcatcgcc agttccacgc caaactcttt cagccctttt tcgctgccac
    34081 agatactctg gaaaatcagc ccgcctggtg cgccgcgacg aatcgcttcg atctgggtgg
    34141 tgacgtgcgc cagtacgcag ccctgagttg ggatgttgaa tttgtcgatc acgccataaa
    34201 tggtatccag cacgcggctt aagttttcca cgtcgtctgt caccgggtta acgccgatca
    34261 ccgcatcgcc cactccgaag gaaagccctt cgtagatttg cgcggcgata ctttgcacgt
    34321 cgtcacgggt gtcatttggc tgcaaacggg cgctaaaggt gcccggaata ccgatggtgg
    34381 tattggcctt tttgattacc ggcattttct tcgcgccgta gatcaggtcc gcgttggagc
    34441 aaatcttcgc taccgccgcg accacttccg aggtcagccc tttgcgggta aaggcaatgt
    34501 cgtccacgct ggtttcatcg ctcagcacat actcacgcag ttcgctgatg ctccagtttt
    34561 taatctggtt gtaggccgtt tcgttaacat cgtcctgaat cagccgcgtc acgcagtcat
    34621 cttcataggc aatcaccgga ttattgcgga tgtccgctac ggtcatttcc gacaacacct
    34681 gctttgccgc cacgcgctcc tgtgaacttg ccgctgcaac gcctgccagc acatcccccg
    34741 aacgcagttc gttggcttta gccagcacct cttttacatc cttaaactga tatacattgc
    34801 cgaacaatgt ggtctttagt ttcataagtc gttccctcag gaaggaaatg cgagtgattt
    34861 caccgtcacc ggcacaaccg atccgccaaa aagaggcgta ccaatgtcga tatagtcccc
    34921 cgcgcggaca atcacttcgt caatgactgc caacgggagt tgttgtagct gtgggcgcaa
    34981 caacatgccc agagctttac caaagtcctg cccggccacc accagcaggg gatgcggatt
    35041 cggaaaacgc gcgacgaaat cgaccagcgc gttgatgacc gtcagtaccg cggcgtaacg
    35101 cacaggcagc gaggcgggaa gcgccagcac gtacgcgtca gttttgggat caagatccag
    35161 ctgaatcagc gcctgttgcc aggcactcac cagatccgtt tcatcaattg ggatcgccac
    35221 cggcaaattg cgcagcggca gttgtacgcc ctccagccag attgtgctgc cagagagcga
    35281 aagggtatgt gcgcccgcgc caatcaccgt ggcgcgtacg gtttgcgccg gaaactgcac
    35341 attcatctca cgcaggcgcg gatggtcatg cagcgtcgtt gccagcagcg ggccaatatc
    35401 ggcaaaacag aacggatcgg cgggctggtg gcgataacat tcgcccacgc cgccagaaag
    35461 cgtaatgatt tcgggcgtaa cacctgcggg cagcaaaccg gtttgcatta atgcctgcgc
    35521 gagcggcgaa agcgttccgt caatcacttc gacaatcagc tctgccatcc gccgggtaac
    35581 ctgcaccagc tgcgcgccgg tcagcgaacg ggcgtcggtg cctgcaccga agcactcatc
    35641 cacaatcatc tgccccggtt tatgagcgta aaccacgcgc ccctggctgt cggtttccag
    35701 caggcgaccg ccgacgttga ggcaggcagt gccgctgatt tttccggcat cgaacagggc
    35761 gtagttcgcg gtgccaccgc cgatgtcgat attcagtacc cgacacagcc gttgttcaga
    35821 aagggtttgc gccccggcac cgtgaccggc gatcacggat tcgaggtgcg gcccggcgct
    35881 ggcaacgaca aaatcgccca gcgactgaga gagcgccatc accgccgggc gagcattgcg
    35941 ggttttcgcg ctttcaccgg tgatgatgat ggcaccagaa tcaacgcttt ccggcgcaat
    36001 acccgcagcc tgatattgct cgagtattaa ggttttcagt tccgcttctt ttaaaccgcc
    36061 ctgtttatcg acaggggtaa agaacaccgg actttgccag ctaatttcgc gtttaatgaa
    36121 ttcgtagcgc ggcacctgcg acaccgccgc acggttaacc agctccaggt gggagaaaat
    36181 cacctgggtg gtggtggtgc cgatatcgat accgacgctc aatagctggc gagtgttcac
    36241 gattgtgcct ccgcttcggt tttagtcgcg gtcgcgtctt cttttggcac cagcatcatc
    36301 gccacgccaa tcgccgttac gccgccgatc aacttgccga caatcatcgg gaagatcatg
    36361 gcgttcatgt tggcagcggc gaagcctaag tggtcgccca gggcgaaagc agcggaaacg
    36421 gcgaaggcgc agttgatgac tttgccgcgg gtatccatct gcttcatcat gccgaacatc
    36481 gggatgttgt tggcaagcgt tgccaccatg ccggctgccg cgatgttgtt catattcagt
    36541 actttaccga cgctcatcag cggtttttca aaccagcgag tcagcagcag caccattgga
    36601 tacgccccta acagcacgca ggagatagaa ccgataactt caatggcgcg catcacttca
    36661 ccgggtttat cgccaggggc cataaagata ggatccagac cggggatcag ttcccagcca
    36721 agcaggaatt tcactaccgc agcggcaaga ccgagggtga tcaatgcaac gaggaatttg
    36781 gcgaagatct ggaagccgtt gatcattttt tccgggatga atttcagccc cagcgccacc
    36841 agaatcgcaa caatgatcac cgggatcatg ttcatcagga tcagggcgaa agtgaattcc
    36901 accggctgac cgttgatctg cacaccggag tacatagcaa ccagaccacc agcaatacag
    36961 ccaatcggaa tggtcacaat gcccgccagc acgccgagcg ccagataacg acgatcagaa
    37021 ggttcgataa tgccgagcgc caccggaatg gaaaacacaa tcgttggccc catcatcgac
    37081 ccgagaatta acccagagta tagccacgct gctacgtcgc cgcccgccag ctctttggcg
    37141 aggaagaagc cgcccatatc gcacgccagc agcgttccgg cgaacattga cgggtttgcg
    37201 ccgagcattt cgtaaaccgg gataattacc ggcccgagta cgtgagccag taccggtgcc
    37261 agcgcggtca taccgaccat cgccagaccc agagcgccca ttgccataaa gccttcttcg
    37321 aactgaccgc cagatccttc gatactttta ccgaacttac cgaggaaacg agcagaaccg
    37381 ccgaactgcg acaggatcct gtctacggca gctatcagca taaagaacat catgatgtac
    37441 atgatgattt cgttaattcc catcgccttt actccctgtt agttgttatt tattggcgga
    37501 tgcggcgtaa acgccttatc cgccctacat gtgcaatccc gaaggccgga tcagacgcgg
    37561 caagcgtcgc atccggcatt tattgcgccg ctgcgtacaa gccgacaatc tgctccaggc
    37621 tggcggtacg cgggttgctg cgcagacaaa tatcttcctg cgcggcctgc gcccatgcac
    37681 cgtaatgcgc agatgtcgcg ccaacatcgc ccagtcgttt accaatccca acttccgcaa
    37741 tcagctcact taccgcgtta atagcgtcat gatcgtcgga ttttttagtt cgcagtgccc
    37801 gaccaatctg actaaagcgt tcacgacaaa ccatccggtt aaattccatc actgttggca
    37861 gcaacatggc gttcgccaga ccgtgcggaa tatgcagcgc cgcgcccggc tgatgcgcca
    37921 tcgcgtggca caacccaaga cccgcactgg aaaacgccat tcccgccata catgaagcca
    37981 gcaacatgct ctcgcgcgcg gcaaggtcgt gaccgtagcc caccgctttc ggcagcgatt
    38041 tgccaatcat cgcaatggca ccaatcgcca gactgtcggt aaacggtgta gcgttcaggg
    38101 cgctgtatgc ttcaatggca tgggttaacg catcaatgcc ggtcatcgcc gtgacatgcg
    38161 acggcacacc ttcggtcaat gcggcgtcga ggatcgccac atccggcatc agcgaggcat
    38221 gggctaacac ctgcttgcgc ccgctcaccg cgtcgataat caccgttaca ttggtggttt
    38281 cagagccggt tccggcggta gttggaatgg caatcagcgg caagcgcggt tgcagaacgc
    38341 tggtttctga catctctgcc agcgtgctat ccgggttcgt caccagcaac gccacggctt
    38401 tcgccgcatc cagcaccgag ccgccgccaa atgcgatcac cccgtcacaa cctgactcac
    38461 gcaactgcgc cacggctgca cacacgtcgg taatgcacgg ttcgcccacc ggacatggcc
    38521 agagcgtcat ggcgatgcct ttaacagcca ggctgcgcgt cagcccggcg gtcatccctg
    38581 cctgatgcaa aaagctgtct gccatcacga acagatgttt cagcccacgc gtttgcgctt
    38641 gctgtccgca actgctcacc gagcccggac cgcaaagcgt caccggtgga acgctaaatg
    38701 tttttacccg ttgcagattc agggtatcga acgcctgaaa gagcgcggtc tgcaattcat
    38761 tttgcatata gcccctccgc tttctctcgc ccactactgg cgatcgccag cggggtcata
    38821 aacaaactgt gctgcggtaa atgcacttgt aacgccggga attgtttgcg aaacagttcc
    38881 gccacgcccg gttgcataca ggagccgccc gccagccata aatcagtaat gccctgccct
    38941 tcaatatgcc gggcgacaat gtccgccatt ttttcgtaca ccggtttcac cgcgggccaa
    39001 atctcgtcac catgaccgcg cttgtactgc tccgcctctt ccagcgaaat acggcgattt
    39061 ccggcgagag tcagagagat gtgatgtccg ccggtggctt catccgccga gtacgtcact
    39121 ttgccctttt tcacgatggc aatgccggta gtgccgccgc cgatatccac tacaccggcg
    39181 ttgtccagtt gcagcagatc cgcaactgcg gttggctcat ccagcacatg gctcacttcc
    39241 agtccagcag actccagcac gttgatggaa atacgcgggt ccgtacctgg aggaaatgaa
    39301 gtcgccgcat ggctaaaacg gcgaccgaat tgctgctcga gcgtgtcgag atggcgacga
    39361 acaatggtga cagcgccgaa gaaatcccag acgatgccgt cgcggaccac atcggcccag
    39421 tcgaggcata ccgccaccgg ctgaccgtcg cggtcaacaa ccatcgacac cacatcgcag
    39481 gtgcccagat ccacccccag ccacagcgga gattccgtcg cggcaggcgt ctggttacac
    39541 agcgttgccg ccgtttgcag acgtggggtg agccattgtt cgtcgtgcgc catcggttac
    39601 tccttaaaca atgcgaaacg catcgactaa tacacagcga cgcagacgga caaacgtccg
    39661 cgcgctggtt accccttcac cggttggcgt ggtgatggtc atggtggtcc agccttcccc
    39721 gcccagcccc agcccggcaa tgcacggtcc gttcttaacg aaaatgctgg tatcaatggc
    39781 gttcgccatc tggttcatgt tttcgatatt gcgcgagtgc attgccgccg tgtggtggca
    39841 accgccctcc agtttcaccg ccagcgcaat ggcatccgcc acgttggcga cgcgcacgac
    39901 gggcagcacc ggcatcatca gttcggtcac ggcaaacgga tgttctgcag tggtttccac
    39961 aaacagcaaa cgcgtttctt gcggcacgtt caggccgatt gccgccgcga ttttcgccgc
    40021 atcgcgacca acccagtcac ggctgacggt gccttttccg cgctcgtcga tatttttcag
    40081 caacaccggt tgcagctgct gcgcctgttc tgcggtcagt ttcaccgcgt gctggccttc
    40141 catcagacgc atcagttcat cggctacgct atcaacaaca atcagtacct tttcgtcggc
    40201 acaaatgatg ttgttatcga aagaagcgcc tttgacgatg gactgagcgg cacgggcgag
    40261 gtcggcggtt tcatccacca ctaccggcgg gttgccagcg cctgcggcaa tcagacgttt
    40321 attggtgtgt ttacgcgccg cttctactac cgcttcgccg ccggttacca ccagcaggcc
    40381 gatacccgga aacttgaaca agcgttgcgc ggtttcgata tccggatttg ccacagtaac
    40441 cagtaagttt tccggcccac ctgcggcaac aatcgcctgg ttgagcagcg taatcgcccg
    40501 ctgggagact tttttcgccg ccggatgcgg ggcaaaaatg acgctgttgc ccgcggcaat
    40561 caggctgatg gcgttgttaa ttacggttgc cgccgggtta gtggaaggcg tcaccgaagc
    40621 caccacgccc cagggtgcgt tttcaattag ggtcaggccg ttgtcgccag tcagcacttg
    40681 cggagagagg cactcaacgc ctggtgtgcc gcgcgcctga gcgacgtttt ttgcaaattt
    40741 atcttcaacg cgccccatgc cggtttcact gacggcaagt tccgctaaat ctctggcgtg
    40801 tttttcgcct gcttcacgaa tggcagcaat ggctaactgg cgcattgcca cgctttttaa
    40861 cccttgctgg gcgactttgg ctgccgcaac ggcgtcatcc agggacgcga aaacgcccat
    40921 ctcatgaacg gcggcgggcg gcgtgtcact gctttgcatt ttcagcagta ccgctttcac
    40981 cacctgttca atatcctgtt gattcatgat gttctgcctt atttgtggaa aattacctga
    41041 ccgccagaca ccacctcatc gacaatgcca atcacgcaca gatcgaccgg tgacgtttcg
    41101 cttttatgcg cctggcgggc ggaactgcca ctcaccagta acacccactc cccggttccc
    41161 gcgccaatat tgtcgatggc gacggcgcat tgcccgtcag gattaccttg tggatcaatc
    41221 atttccacca tcagcaattt gtcatgcgcc agtccgtgat ggcgtacggt acaaacaatt
    41281 tgtccagtga cgactgccag tttcataccc gcctccgtgg cgtatttcag gtaaaagctc
    41341 cccctaccct ccgcagaagg taaaatgaaa aaggagagag cgtgacgccc gaatcgacgt
    41401 cacacagggt gattacaggt tgctgctatc gcctttcagg ccgatcggga agacttcttc
    41461 caggtcgccg tgcggacgcg ggataacgtg tacagatacc agctcgccga tacgctgtgc
    41521 cgctgcggca cctgcatcgg tcgcggcttt acaggctgca acatcaccac gaaccatggc
    41581 agtacacagg ccgccgccaa tctgcttaac accaaccagc ttgacgcgcg cagctttcac
    41641 catcgcgtca gaagcctcaa tcagtgcaac caggccccgg gtttcgatca ttcctaatgc
    41701 ttccattgtg ctttcctctt tatcagggtc cagaacggga ccgttcattc aaccagtgtt
    41761 tgtaaactgc tttcgcggtt cacttctgtc tgacgcggca cagctgccac cagcgccagc
    41821 tcgataattt cctgcacgct acaaccacga gagagatcgt gcatcggcgc ggcaagtcct
    41881 tgtatcagtg gcccgacggc acgatatccg ccgagtcgtt gtgcgatttt gtaaccaata
    41941 tttccggctt ccagcgacgg aaaaaccatc acattggcct tgccctgtag cgggctggca
    42001 ggcgcttttt gcgccgccac ttccggcacg aaggcggcgt caaactgtaa ctcgccatcc
    42061 accaccagct ttggtgcgcg ctcacggacg atttctgtcg cctgctggac gttagcaaca
    42121 caggggtgac gggcgctacc attgctggaa aacgacagca tcgccacgcg cggctcttct
    42181 ccggtgatgg cgcgccaggt ttcggcactg gcaagcgcga tatccgccag ctgcgccgcc
    42241 gtcggctgtg gcaccacgct gcaatcggca aagcccaacg ccgggccgct gtactgtggc
    42301 aacatcagga aaatggaaga gagcgtttta cagcctggct gcaagccgat aatgcgtaat
    42361 ccggcacgca gcacatttgc cgtggaagag aggttgcccg cgatacagac atccgcttta
    42421 ccggcgctga ccattgctgc ggcgaacatc agagggtcgg taagtttttc cagcgcatcc
    42481 ggcggcgttt tttcgcccgc gcgggccagc cagcgatgag caaattcttc ccgcattgcg
    42541 aggttgccat gcggatcaat cacctgtagc ccgtccatcg ccacgccgtg actgagcgca
    42601 aactgacgaa gttcaaacgg attggcgacc agaatgggcg ttgccagacc ttgttgatgt
    42661 aaatattgcg cggctttcag cacacgttgg tctaacgcat ccggaaaaac cactctggcg
    42721 ggcgctcgca acgccagttc acgacaacgt tcaataatca ttgcgtttcc cccagtcgtt
    42781 gttggatctg cttcaccgtt agcggctgct tcgttacgct caggatcatc atcacgtaga
    42841 ccgtgctgga aaggcggttc agcgcctgca aaatgtccgg gcgtaatact tcaaagctgc
    42901 gggtgataaa cacctgcgcc gctacggttt cggtttcccg cactttggta cgtagcagat
    42961 tgagcaacgc cgcatcgcgc ccgtggctgg cttccggcac cagatgatcg tggtcgagat
    43021 agcgcagcgg ctggtgagaa agtcgatgca gatcttcgtc agaaagcccg acaatcgcct
    43081 ggcagcccag cggttcaccc agcgcatcgg cgcgcataat gttgccaaga cgcgaacgga
    43141 tatccgccag ccacggctgc cacggttccg ccagttcaat ttgcagccac actgccagcg
    43201 caatggtgct gtcgagaacc gcgcgaaaac ccagacgcgg atcgcttttg gcgaccattt
    43261 tttccgccga caggtgagtc agcgtatccg gcttttttgc caccggctgg cggcacagtt
    43321 cgcagcacgc ctgcggatgt tcatcgctac tggtcagccc atgaacgggc tgcggctgct
    43381 gctgttcatc gtcaacaaac aggcggccct gctcgtcaat aaacttgatg cgcagatggc
    43441 ggctttccag taactcccgg gcagagggcg tcaggcgact gtccgcaggg agatggatct
    43501 ctgcgccttc gctgagcgta tggttcgctc ttagccatgc ttcggtgatg aaatctttca
    43561 tagggattgc cagttagccg gccaggcgac atacaggaat ttcacgctgg atgtcgtacc
    43621 aaattcgata ctggagcctt tcgggataaa catcacgtca cccgctttgg cgatcatggt
    43681 ttcgccttcg tggcgaacat gcagctcccc ttccagcacc atgtcgattt cgtcgtagtt
    43741 cagggtccac gggaagaatg cgttctccca ctgcataaaa ccagcggcca tgctgctgcc
    43801 gtcgtctccg gtcacaagat cagtcaagcc cacgcaatgc ggctctgcgc cgtcgaaacg
    43861 accaaacttg acgctgctgc catcaatcac tttgatgccg cctttgccgg tcaccgattt
    43921 gaagctcggc tgcattgcgc cctgctccag cgactgtttt tccttcatca ctttttccat
    43981 cagctgtgcg accaggcttt cggtaaactg gccttccggc agctgggcaa tgatggtttc
    44041 gcggatgcgc tggctttcgg ttttgtcggc tggcacgctg gcgggcacgg acgccgttac
    44101 cgggatcgat tcatcacatt cggtgatggt aaaacccagt agatccgcca cttcacgggc
    44161 ttccggggta ataatgctgg cgcgcagaac cacggacatt gcctgttcgc cgcgcgcgtg
    44221 tgcttcacga atatcattcg ctgtgatgag ttttttcacc tgcttcctcc tgtttggtga
    44281 gagatgccag ataatccacc agttgctgca cgctttgcgg gtcgtggctg tttagctcga
    44341 aaatcggctc ttcgaaaccc gtctcaagca ataactttcg cgttgcggcg acgtcagcat
    44401 ctggcatgtc cgttttactg atgacggcga tttgccgttt gctgacgcca atatccagca
    44461 acccggcagg caaccgactt tccgggtcat ttgcgccatg gacatagatc agcatgtcta
    44521 catcctgtag cgtggtaatt aaggcgtgat accagcgggg atggctaaaa tattcacccg
    44581 gcgtatcaat atcgccctta tcattaaatt ccacggcctg tgtttttctg gcgagggtat
    44641 aattcccctg caacgcatta aataacgttg tttttcctgc cccaacgcta cccacaaacg
    44701 caattcgttt catgaatgcc tcgtattaac ttttggtcat ttcacaaagc gtataattta
    44761 ataagcgacc taaaccgctg acggtttgtg ataacgcttc ttctactgcg cccaccgagc
    44821 cataaatcac caatgcaccg ctgaagcggt cgagaaaacc gatatgcaca tcagcggctt
    44881 tcagcgctaa atcaccggcg atcatcgccg tttcgccggg agttagcgtc ataatgccaa
    44941 ttgcgcccgc atccggaacg ccgattttct tcgccagttc ctcaccggga tgcgcgatga
    45001 gatgcgccag tgtgacctgt ttgccgggca caaattcctg aatgatgcgt tctttatcca
    45061 tgagtcgccg cctgtgaaaa actttcgtgt atcgtgacaa tttttcgtca gagtaaataa
    45121 caaaattcgg caagcgcgtt taaaaggtga ggtagatcac taagaatgga gagatattcg
    45181 ctgtggtgca taaactttca tatgcaacgc atgaatataa ataaaataat atcgagatat
    45241 tatatagact gcttttttta ttattcgcaa actcagtaat aagctttact aatatattgc
    45301 agccaaaccg cttaccctga atattcaggg taagcgtgag agttaaaaaa gaattacagc
    45361 ggttgggttt gcgcttcaac cacggccagc gccaccatgt tgacgatacg acgcaccgat
    45421 gcgatcggcg ttaacacgtg aaccggtttc gccacgccca tcagcaccgg accgacagtc
    45481 acaccttccg agctggaaac gcgcagtaag ttgtaactaa tgcgggcagc ttccatgttc
    45541 ggcatcacca gaatattggc agaacctttc aaagggctgt ccggcatacg gtcgttgcga
    45601 atcgcttcca ccagcgctgc atcgccgtgc atttcaccat caatcatcag atctggcgca
    45661 cgatccctga ccagttccag cgcctgacgc attttgctcg acgacgggca gtcagaagaa
    45721 ccaaagttgg agtgcgacaa cagagcaacg cgcggctcaa taccaaaacg acggacagtt
    45781 tctgccgcca tcaaggtgat ctccgccagc tcttctgcgt ctggttcgtc attgacgtag
    45841 gtatcggcaa taaaggtgtt accactcagc agcagcagcg cgttcattgc acctgcggtg
    45901 tgaacgccat cgcgataacc aaagacattt ttcaccacgc taaaatgttc atgataatcg
    45961 cccaccgtac cgcagatcat tgcatcggct tccccacgct gaaccatgat cgcgccgatc
    46021 actgtcgggt tactgatcag cgcccgctgc gcctgttcct gagtgacacc gcgacgcttc
    46081 atgatctgga agtattcggt ccagtactct ttaaagcgcg gatcggattc gttattgacg
    46141 atctcaaaat caacgcccgc tttgatctgc aagcccagtt tctgaatgcg catttcgatc
    46201 acgttcggac gaccgataag gatcggtttc gccagtccca gcgttaccag ttcctgagtg
    46261 gcatgcagaa cgcgcgcctc ttccccttct ggcagaacaa cgcgcttcgg cgctttgcga
    46321 gcctgggaga aaatcggctt cataaacagg ttggttttgt aaacgaactc agtcagcttg
    46381 tcgatgtaga cgtcgaaatc agcaatcgga cgcgtcgcca cgcccgactc catcgcggct
    46441 ttagcgaccg caggagcgat cttaacgatc aagcgcggat caaacggttt tggaatgatg
    46501 tattccggac caaagctcag atcctgatcg ccatacgctg aagccaccac ttcgctctgt
    46561 tcggcgtggg ccagttccgc aatcgcgcgt accgccgcca gtttcatctc ttcattgata
    46621 gccgttgcgc caacatccag cgcgccacgg aagataaacg ggaagcaaag gacgttgttg
    46681 acctggtttg gatagtcaga acgaccggtg caaatgatgg catccggacg cacttctttc
    46741 gccagcggcg gcagaatttc cggttccggg ttcgccagcg ccaggatcat tggcgcacga
    46801 gccattttct tcaccatttc ctgggtcagc actttcgggc cggaacatcc aaggaaaata
    46861 tccgcgcctt caatcacatc atcgagggta cgtttgccgt catccaccac cgcatatgcg
    46921 gctttggttt ccgccatgtt tggctcacgg ccctgataga taacgccttt tgaatcgcaa
    46981 accacgatgt tatgtttttg cagacccagc gctaccagca ggttcataca ggcgattgct
    47041 gcggcacccg cgccggaaac caccatccgc acgtcggaga tgtttttctc caccacgcgc
    47101 aagccgttga ggatggcggc agtgctgatg attgccgtgc cgtgctgatc gtcgtggaat
    47161 accggaatat tcatccgctc gcgcagtttc tgttcaatat agaaacattc tggcgcttta
    47221 atgtcttcga ggttgattcc gccgaaggtc ggttcgagcg cagcgacaac ttcaataaat
    47281 ttgtccgggt cgagttcatc aacttcaatg tcaaatacat caatcccggc gaatttctta
    47341 aacagaacgc ccttgccttc catcaccggt ttgccagcca gcgcgccaat gttgcctaac
    47401 cccagcaccg ccgtaccgtt agagatcacc gccaccaggt taccgcgcgc ggtatatttg
    47461 taggctttta acgggtcttt ttcgatttca agacaaggtg cggcaacgcc tggtgagtag
    47521 gccagcgcca gatcgcgctg cgttgccaga ggcttggttg gagaaacctg gattttccct
    47581 ggaactggaa attcatggaa atcaagtgca ctttgtttta actggtcatc catttggttg
    47641 ttcctttcac gtaacgttca caaataaagt gtgtgggcaa cagcccctgc ccacaacgtg
    47701 gcgcacatta ttaccctgcc ggagtctaca gactttgagc aagtccaaac tctcaccatt
    47761 aatataatgt tttggtaata atcctataac actgatgtta cctgcttaat ccagcaatac
    47821 catgcctgtc tgctatgctt ttttgatgcg tttagcgaaa tttctcagaa gtgtgaatta
    47881 acgcactcat ctaacacttt acttttcaag gagtatttcc tatgaacgag ttagacggca
    47941 tcaaacagtt caccactgtc gtggcagaca gcggcgatat tgagtccatt cgccattatc
    48001 atccccagga tgccaccacc aatccttcgc tgttactcaa agctgccgga ttatcacaat
    48061 atgagcattt aatagacgat gctatcgcct ggggtaaaaa aaatggcaag acccaggaac
    48121 aacaggtggt cgcagcgtgt gacaaactgg cagtcaattt cggtgctgaa attctcaaaa
    48181 tcgtacccgg tcgcgtgtca acagaagttg atgcacgcct ctcttttgat aaagaaaaga
    48241 gtattgaaaa ggcgcgccat ctggtggact tgtatcagca acaaggcgtt gagaaatcac
    48301 gcattttgat caaactggct tcgacctggg aaggaattcg cgcggccgaa gagctggaaa
    48361 aagaaggtat taactgcaac ctgacgctgc tgttttcttt tgcccaggcg cgggcctgtg
    48421 cagaagcggg cgtttttctg atttcgccgt ttgtcgggcg tatttatgac tggtatcagg
    48481 cacgcaagcc gatggacccg tatgtggtgg aagaagatcc gggcgttaaa tcggtgcgca
    48541 atatctacga ctactataag caacaccact atgaaaccat tgtgatgggc gcgagcttcc
    48601 gtcgcaccga acaaatcctc gccttaaccg gctgcgatcg actgactatc gcaccgaatt
    48661 tgctgaagga gctgcaggaa aaagtttcgc cagtggtacg taaattaatc ccgccttctc
    48721 agacgttccc acgcccagca cccatgagcg aagcggagtt ccgttgggag cacaatcagg
    48781 atgcgatggc ggtagaaaaa ctgtctgaag gcattcgtct gttcgctgtt gatcaacgca
    48841 aactggaaga tcttcttgcc gccaaactat aaaccagcca cggagtgtta tatgtcccga
    48901 aaagaccttg ccaatgcgat tcgcgcactc agtatggatg cggtacaaaa agccaattct
    48961 ggtcatcccg gcgcgccgat gggcatggct gatattgccg aagtgctgtg gaacgatttt
    49021 cttaaacata atcctaccga cccaacctgg tatgatcgcg accgctttat tctttccaac
    49081 ggtcacgcgt cgatgctgct ctacagtttg ctacatctga ccggttacga cctgccgctg
    49141 gaagaactga agaacttccg tcagttgcat tcgaaaaccc caggccaccc ggagattggc
    49201 tatacgccag gcgttgaaac caccaccggc ccgcttggac aaggtttggc gaacgccgtc
    49261 gggctggcga tagcagagcg tacactggcg gcgcagttta accagccaga ccatgagatc
    49321 gtcgatcact tcacctatgt gtttatgggc gacggctgcc tgatggaagg tatttcccac
    49381 gaagtctgtt cgctggcagg cacgctggga ctgggcaagc tgattggttt ttacgatcac
    49441 aacggtattt ccatcgacgg tgaaacagaa ggctggttta ccgacgatac ggcaaaacgt
    49501 tttgaagcct atcactggca tgtgatccat gaaatcgacg gtcacgatcc gcaggcggtg
    49561 aaggaagcga tccttgaagc gcaaagcgtg aaagataagc cgtcgctgat tatctgccgt
    49621 acggtgattg gctttggttc gccgaataaa gcaggtaagg aagaggcgca cggcgcacca
    49681 ctgggggaag aagaagtggc gctggcacgg caaaaactgg gctggcacca tccgccattt
    49741 gagatcccta aaaagattta tcacgcctgg gatgcccgcg aaaaaggcga aaaagcgcag
    49801 cagagctgga atgagaagtt tgccgcctat aaaaaggctc atccgcaact ggcagaagag
    49861 tttacccgtc ggatgagcgg tggtttaccg aaggactggg agaaaacgac tcagaaatat
    49921 atcaatgagt tgcaggcgaa tccggcgaaa atcgccaccc gtaaggcttc gcaaaatacg
    49981 cttaacgctt acggaccgat gctaccggag ctgctcggag gttcagcgga tctggctccc
    50041 agcaacctca ccatctggaa aggctctgtg tcgctgaagg aagatccggc gggcaactac
    50101 attcactacg gggtgcgtga atttggcatg accgctatcg ccaacggcat tgcgcaccac
    50161 ggcggctttg tgccgtatac cgcgacgttc ctgatgtttg ttgaatacgc ccgtaacgcc
    50221 gcgcggatgg cggcactgat gaaagcgcgg cagattatgg tttataccca cgactcaatt
    50281 ggcctgggcg aagatggtcc gacgcaccag gctgttgagc aactggccag cctgcgctta
    50341 acgccaaatt tcagcacctg gcgaccgtgc gatcaggtgg aagcggcggt gggctggaag
    50401 ctggcggttg agcgccacaa cggaccgacg gcactgattc tctccaggca gaatctggcc
    50461 caggtggaac gtacgccgga tcaggttaaa gagattgctc gtggcggtta tgtgctgaaa
    50521 gacagcggcg gtaagccaga tattattttg attgccaccg gttcagagat ggaaattacc
    50581 ctgcaagcgg cagagaaatt agcaggagaa ggtcgcaatg tacgcgtagt ttccctgccc
    50641 tcgaccgata ttttcgacgc ccaggatgag gaatatcggg agtcggtgtt gccttctaac
    50701 gttgcggctc gcgtggcggt ggaagcaggt attgccgatt actggtacaa gtatgttggt
    50761 ctgaaagggg caattgtcgg gatgacgggt tacggggaat ctgctccggc ggataagctg
    50821 ttcccgttct ttggctttac cgccgagaat attgtggcaa aagcgcataa ggtgctggga
    50881 gtgaaaggtg cctgatggtg atcgccggat gctgattgcc ggatgcgacg ctgacgcgtc
    50941 ttatccggcc tacagcgtct tatccggcct acatgtcccc gcaattttgt ttaacgggtg
    51001 atccacaacg tgggccaggc gtctggccca tgccagttat cgcaggtggg ttctgcggca
    51061 taacgcacca ggcgaaaacg ctgaccgtca aagcgccagc gcgcctgaat gccacaatcg
    51121 cttaatccgc gccctttcgc taaggtcacc agttcacgcg atttctcatc aaatgttgcg
    51181 ttcatcagtt ccagttcatt cgtctcctga ccgttgttga acggcaaacg caaccgaacc
    51241 gggcgcgagg ctagtggctt tttacgcgac acaatccatg ccaaatcaat ggtgttatag
    51301 gcccctgcct cacagctaat catcatcagc gctttatcat cagtcagcgc agtgacattc
    51361 acctcacgac gtaacggatc aagcgagcag cgcagaccat tcatccgcca gtttccataa
    51421 tccagcaaat cgttgcgttc ttcgagtgag agtggtgtcg gcgttgggtt aaccaccgcg
    51481 acctctttca gcgcaggcgc gggcggtacg ctgagcggcg gttcgtcccc tttcttgatc
    51541 cacgcggttt cactgccaac gcgcttttgc tgagcatcaa taaacaacaa cgccgctttt
    51601 aagccactca gagaaatggt ctgattgccg tcgcgtaagg tgatcgcctt cccttcctga
    51661 atcatctgca aaaacgcggt gatggttgcc gtatcatcgg ttactaataa ccatggtgaa
    51721 atccgccact tgtcaccact taacgccaac ggctcaccat ctaacagcag ccgtggcgct
    51781 atctctcctt cagacgcatc cggcgacttc aacccgccgc gctcaatacg taaaacggca
    51841 tcggtatgcg ccccggcgct gcggctcagg gtcatcacca gtccattatg atcgcccgta
    51901 ttgcgcgcca cgcagaagtt ttggttatta caggtgacct gccagtcgga aaacgcccgt
    51961 tgcgctggtg ccgcccacac caaagacgtc ggcaacaaag cgaaaaaaaa gagaaggaaa
    52021 atgcgatagc gcatggacgg tacgacccca gaagttcaaa caaacagcac aagtcgtatc
    52081 ttcgttgcgc aggtagcgcg gctcaatcag atttgtcgga ttgagccaat aataaacaac
    52141 gaaatattat tgttattttt cagtccatta aatgtgacgt ttgcaaatag ttaagcaata
    52201 acaccgtctt accgtcacgt atctcgccgg ttttgatcat ctccagcgcc tggctgaacg
    52261 gcagctcgag cacttcaata tcttcatctt caacaccgcc accggcgtta gcgcgctgat
    52321 tgtcactgta ttcggcgata aaaaagtgga ttagctcagt cacaccgcca ggcgacatat
    52381 acagttcaaa taatttgcgc acttcgccca cttcatagcc cgtctcttca atcgcttctt
    52441 tacgaatgca cacttccggt tcgtcgttat ccagcagccc ggcgcaggtt tcaatcagct
    52501 gcccgctttc attgccatta acccaggtag cgacacggaa ctgacgaatc agaaccacgc
    52561 tctttttctt cgcgttgtac aggaggatcg tcgcaccatt accgcgatcg taaacttcac
    52621 gtttatggcg gataacttcg ccatctttgc gcgtgagatc gtaggtaatg ttgtgcaggg
    52681 tgaaatagtt atcggagaga attttgtctt taatgagggt gatttgttgc gtcataccga
    52741 ctccacagcg cgaaatgaac aattatctta cgctgtgaag tcgggtttgt ctgcgcaggc
    52801 tatcagtgag atttgactga ttttaccccc agccagtcaa taatcccctg tgccgcatga
    52861 cgtccttctg ccattgcggt aaccaccaga tccgcaccac gcacggcgtc accaccagcg
    52921 aagattttcg gattggtggt ctggtaacgg tactggcttt ccacatccgc gatgatgcgg
    52981 ccccatttgt ctaccgttac accgtgcgac tccagccacg gcatcccgtg cggattgaag
    53041 ccaaacgcca taatcaccgc gtcggctggc atgacaaatt cactgccttc caccggcact
    53101 ggacgccgac gcccctgggc atccggctcg ccaagacgcg tgcgcaggaa acgaatcccg
    53161 cagacgtgac cttgttcatt cagctcaagc gccaccggct ggacgttaaa ttcgaagttg
    53221 gccccctctt cgcgggcgtt cctcacctct ttcttcgagc ctggcatgtt agcttcatca
    53281 cgacgataag cgcaggtgac gttactcgcg ccgtggcgca gcgcagtacg cacacagtcc
    53341 atcgcggtgt cgccgccgcc cagtaccacg acgttaagtc cggcggtatt gataaacggc
    53401 tcttccggta gctcttcgag ccccatcacc tgtttagtgt tggcaatgag gaacggcagc
    53461 gcgtcataaa cgcccggcgc atcttcattg ggtaaacccg ctttcatgga acggtaagtg
    53521 cctacgccaa cgaagaccgc gtcgtattgt tccaaaagcg aatccaaaga gacatcttta
    53581 cccacttcac aattgagttc gaagtgaatc cccatcgcgc tgaagatttc ccgacggcgt
    53641 gccagcaggg atttatccag tttgaaagaa ggaatgccga aagtgagcaa gccaccgatt
    53701 tctggatggc gatcgtacac cgtcaccccc acgccattgc gggtcagaac atccgcacag
    53761 gccagccctg ccggacctgc accgataatc gccacccgct tgtccacttt ggtgacatgg
    53821 cttaagtcag gacgccaacc tttcgccaac gcctgatctg aaatgtagcg ttcaatgttg
    53881 ccgatagtta ccgcgccgtg ctcatcgcga atagtacagg caccttcaca caaacggtct
    53941 tgcggacaaa cgcgtccggt aatttccggc agggtgttgg tctggtgaga aagctcgacg
    54001 gcggcgtcga tgtttccggc tttcaccagt tcaatccact gcggtatatg gttatgcagc
    54061 gggcaggtcc attcacaaac gctgtgctcg ccgcacttaa ggcagcgcga ggcttcccgt
    54121 tgtgcctggt cggcgcgaaa tggcagataa atttcatcaa aaccggtttt gcgcgcttca
    54181 atcgccagtt tatccggctc gccacgcgcg ggcgttgcct gcatttgttc gactttactc
    54241 attaccggca tttcttgcgc cgcggtactg gcatgccacg gttgatgttc ctgacgcgcg
    54301 gtgcgcaagc ggcgggattt cgccatgccg gagagtgcga cgtcagtgac cagttgcagc
    54361 gcgtccgccg ggcaattctc aacacaggca ggaccgtttt cgcgccccgc acaaaggtcg
    54421 catttatgcg ccgtggcttt tacttttcct gccgcgacgg gtgtcaggac gatttgcatc
    54481 gtaccaaaag gacaggccac cacgcaggat ttacagccaa tacacttttg ctgattgacc
    54541 tgaatgctgt catcaacgtg gctgattgcg ccattagggc agctacgggc gcagggcgca
    54601 tcttcacaat ggtgacaggt cactgcacta cgttgctgtt gatgtttgat aaccgtaatt
    54661 cggggatgaa aatggtgttg gctcaggaca tgttgctcat cattgtgagc catgacacag
    54721 gcgatttcac aagcatgaca acccagacat tgctgactgt tggccataat aaaacgattc
    54781 ataacgacct tcttttttgg ttgtaaaaac cttattcttt atatgagtgt tgttattacc
    54841 cgacttacag gggaatcggc aatgttcatg tgcccagaat aagtaactat ttcgctataa
    54901 actgtggcag atcaaataat cccatcactg actaaattgt gtttcagcga actggaacat
    54961 taatgatttt ttgtggagaa gacgcgtgtg attgttaaac gacccgtctc ggccagtctg
    55021 gcccgggcct ttttttacat tgtgctgctg tcgattcttt ccacgggtat cgctctgcta
    55081 actctggcga gcagtttgcg cgacgctgag gctatcaata ttgccggatc gctgcgtatg
    55141 cagagttacc gcctgggcta cgacttgcaa agtggcagtc cacaactcaa tgcacatcgc
    55201 cagttgtttc agcaggcact gcattcaccg gtattaacca atctcaacgt ctggtatgtg
    55261 ccagaagcag taaaaactcg ctatgcgcat ctgaatgcca actggctgga gatgaataat
    55321 cggctcagca agggcgattt gccgtggtat caggccaata ttaataatta tgttaatcag
    55381 atagacctgt tcgtgctggc tttacagcac tacgctgaac gcaaaatgct gctggtggtg
    55441 gcgatttccc tggctggcgg tatcggtatt ttcacgctgg tcttttttac tttgcgccgt
    55501 atacgccatc aggtggttgc cccgctgaat cagctggtta ccgccagtca acgtattgaa
    55561 cacgggcagt tcgactcgcc gccgctggat accagtctgc cgaatgagct tggtctgctt
    55621 gcaaaaacct ttaaccagat gtcgagcgag ctgcataaat tgtacctttc gctggaggcg
    55681 tcagtagaag aaaagacccg cgatctccac gaggccaagc gtcgtctgga ggtgttgtat
    55741 caatgttccc aggcgctgaa caccagccag attgatgtgc attgtttccg ccatattttg
    55801 cagattgttc gcgacaatga agcggctgaa tatctggagt taaatgtcgg tgaaaactgg
    55861 cggattagcg aagggcaacc aaacccggaa ttgccgatgc agattttacc ggtgacaatg
    55921 caagagacgg tttacggcga actgcactgg caaaatagtc acgtttcatc atcagaaccg
    55981 ctgcttaaca gtgtttcatc gatgctggga cgcggtttgt actttaatca ggcgcagaag
    56041 cattttcagc agttattgtt gatggaagaa cgtgcaacca tcgcccgcga attgcacgac
    56101 tcgctggctc aggtactttc ttacttacgt atccagttga cgttactgaa gcgttcgata
    56161 ccggaagata acgccaccgc acaaagtatc atggccgatt tttcccaggc gttgaatgat
    56221 gcttatcggc agttacgcga gctgttgact actttccgcc tgacgctgca gcaggcggat
    56281 ctcccctccg cgttgaggga aatgctggat acgttacaaa atcaaaccag cgccaaactg
    56341 accctcgact gccgtctgcc aaccctggcg ctggatgcgc aaatgcaggt gcatttgttg
    56401 caaattattc gcgaagcggt gctgaatgcg atgaagcacg ccaacgccag cgaaatcgcc
    56461 gtcagttgcg tcaccgcgcc ggacggcaat cacacggttt atatccgtga taacgggatt
    56521 ggtatcggtg aaccgaaaga acccgaaggt cattatggtc tgaatatcat gcgcgaacgc
    56581 gcggaacggc tgggtgggac gctgactttt tcacaacctt ccggcggcgg cacgttagtg
    56641 agtattagct ttcgctctgc ggagggtgag gaaagtcagt tgatgtaatg cctcctactg
    56701 accaaagaat acttgcactt aaggttcagt ataaaagggc atgataattt acattaactc
    56761 ctttttttct ccacgattgg ctcgtacctt gccgctacag tgaagcaagt caagcctaca
    56821 acgatacgca gaaacacgag gtcctctttt aatggcgaat ttctttattg atcgccccat
    56881 ttttgcctgg gtgctggcaa tcctgttgtg tctgacaggg accctggcga ttttttcatt
    56941 gcccgttgaa caataccccg atctcgcgcc accgaatgtg cgagtgaccg ctaactatcc
    57001 cggcgcatcg gcccagacgc tggaaaacac cgtgacccag gttatcgagc aaaatatgac
    57061 cggcctcgat aatctcatgt atatgtcatc tcagagcagt ggcaccggtc aggcatctgt
    57121 cactttaagt tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag tacaaaacca
    57181 gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg tgacggtgcg
    57241 taaaaccggc gataccaata ttctgaccat tgctttcgtc tctaccgatg gttcgatgga
    57301 taaacaggat attgccgatt atgttgccag taatattcag gacccgttaa gccgcgtgaa
    57361 tggcgtcggg gatatcgatg cctatggttc gcaatattcc atgcgtatct ggctggaccc
    57421 ggcgaaactc aacagtttcc agatgacggc taaagatgtc actgatgcca ttgagtcaca
    57481 gaacgcgcag attgcggttg ggcaacttgg tggtacacct tccgtcgata agcaggcgct
    57541 caacgccacc attaacgccc agtcactgct gcaaacacca gaacagttcc gcgatatcac
    57601 cttgagggtg aatcaagacg gctcagaggt aaggctgggc gatgtcgcca ccgtcgaaat
    57661 gggggcggag aaatacgatt atcttagccg cttcaatggt aagccagcct ccgggctggg
    57721 ggtaaaactg gcctccggcg caaacgaaat ggcgacagcg gagctggtgc tcaatcgtct
    57781 cgacgagctg gcgcagtatt tcccgcacgg actggaatac aaggtggcgt atgaaaccac
    57841 ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag ctatcgctct
    57901 ggttttcctc gttatgtatc tgttcctgca aaacttccgc gccacgctga tacccactat
    57961 cgccgtgccg gtggtgttga tgggaacctt ctccgtactt tacgcctttg gttacagcgt
    58021 caacacctta accatgttcg cgatggtgct ggcgatcggt ctgctggtgg atgacgccat
    58081 cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa ggactcactc ctcgcgaagc
    58141 cacacgtaaa tcgatggggc agatccaggg ggcactggtc gggattgcga tggtgctttc
    58201 ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct atcgccagtt
    58261 ctctattacc attgttgcgg cgatggtgct gtcagtactg gtagcgatga tcctcactcc
    58321 ggctctgtgt gccacactac ttaagccact gaaaaaaggt gagcatcacg ggcaaaaagg
    58381 cttttttgcc tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg aaaaaggggt
    58441 ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc tgcttggcgg
    58501 catggtgttc ctgttcctgc gtttgccgac gtcgttctta ccgctggaag accgtggcat
    58561 gtttactacc tcggtacaat tgcccagcgg ttcaacccaa caacagaccc tgaaagtcgt
    58621 tgagcaaatc gagaaatatt acttcaccca tgaaaaagac aacatcatgt cggtgtttgc
    58681 cactgttggt tctggccccg ggggtaacgg gcaaaacgtg gcgcgaatgt ttatccgcct
    58741 gaaagactgg agcgaacgcg acagtaagac cggcacctcg tttgccatta tcgagcgtgc
    58801 aacaaaggcg tttaacaaga ttaaagaagc tcgcgttatc gccagcagcc cgccagcaat
    58861 tagcggtctt ggtagttctg cgggttttga tatggagttg caggaccacg ctggagcagg
    58921 tcacgatgcg ctgatggcag cacgtaacca gttgctggcg ctggcggcgg aaaacccgga
    58981 gctaacccgt gtgcgccata acggcctcga cgacagtccg cagttgcaga ttgatatcga
    59041 ccagcgtaaa gctcaggcgc tgggcgttga tatcgacgat attaacgaca cactgcaaac
    59101 cgcctggggt tcgagctatg ttaatgactt tatggatcgc ggtcgcgtga agaaagtcta
    59161 tgtgcaggca gctgcgccgt atcgcatgct gccagatgac atcaatctct ggtatgtccg
    59221 aaataaagat ggcggcatgg tgcccttctc tgctttcgcg acctcacgct gggagacagg
    59281 ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt gagattgttg gggaagccgc
    59341 accgggggtc agtaccggta cggcgatgga tattatggaa tcgttagtga agcagttgcc
    59401 aaacggcttt ggtctggagt ggacggcgat gtcgtatcag gagcggcttt ccggcgcgca
    59461 ggctccggcg ctgtacgcta tttccttgct ggtggtattc ctgtgtctgg cggcgttgta
    59521 tgaaagctgg tcggtgccgt tctcggtaat gctggtcgtg ccgctggggg ttatcggcgc
    59581 gctgctggca acctggatgc gcgggctgga aaacgacgtt tacttccagg tgggcctgtt
    59641 aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg ctaacgagat
    59701 gaaccaaaaa ggccacgacc tgtttgaagc gacgctccac gcctgccgtc agcgtttacg
    59761 cccgattctg atgacctcgc tggcatttat cttcggcgta ttgccaatgg caaccagcac
    59821 gggtgccggt tccggtggtc agcatgcggt gggtactggc gtaatgggcg ggatgatttc
    59881 ggccactatt ctggctattt acttcgtgcc gctgttcttt gtactggtgc gccgccgctt
    59941 cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg acatgctaat gtgtcgcctt
    60001 tttcaacttt ccgattaaga acctgctcag cgggttcttg ctgtttgtac tttgtctcag
    60061 gaattactta cgaagcataa cttcgataaa gtctttccag ttccccagtt cacgttcaat
    60121 cataacaacc tctcttataa ttatgggtat tctacggaaa caatataccg tggtgaagct
    60181 aatttactcg attgctgcga tgactacctc cgggggacaa accttatgta aatactatgg
    60241 tcctgcggtg ataatttgta tgtgatacac agcaacattt cgagatattc atacggcatc
    60301 taatactgat ttaattctgg ttaaaataca gacagataac aag
//
LOCUS       DAPHDW010000009        59700 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.009, whole genome shotgun sequence.
ACCESSION   DAPHDW010000009 DAPHDW010000000
VERSION     DAPHDW010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59700)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59700)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59700
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.009"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(186..1085)
                     /gene="pgrR"
                     /locus_tag="RH057_000548"
     CDS             complement(186..1085)
                     /gene="pgrR"
                     /locus_tag="RH057_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HDV3126964.1"
                     /translation="MKREEIADLMAFVVVAEERSFTRAAARLSMAQSALSQIVRRIEE
                     RLGLRLLTRTTRSVVPTEAGEHLLSVLGPMLHDIDSALASLSELQNRPSGTIRITTVE
                     HAAKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSERFDAGVRLGGEMDKDMIAIRI
                     GPDIPMAIVGSPDYFSRRSVPTSVSQLIDHQAINLYLPTSGTANRWRLIRGGREVRVR
                     MEGQLLLNTIDLIIDAAIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYY
                     PHRRHAGSAFSLFIDRLKYKGAV"
     gene            1223..2143
                     /locus_tag="RH057_000549"
     CDS             1223..2143
                     /locus_tag="RH057_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3126965.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYQAIVVSHPGG
                     GVKEQTAGTYAKKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDISAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPYVEAGSNARTSDISSGEYAVMPLAPMKESDAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            2143..3075
                     /locus_tag="RH057_000550"
     CDS             2143..3075
                     /locus_tag="RH057_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3126966.1"
                     /translation="MKHIQIRNSDMAWHIAANIQFPPNFDESKQYPAIISVHPFGSCK
                     EQTSGNIYGKALAEKGYLVLAYDASFQGESGGEPRWIEDPTQRVEDISRVIDYAVTLP
                     YVNAERIGVLGVCGGGGYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGALN
                     AMASQRTKEARGGELQINELLPASLDAAKSHGLTERDVYEATDYYKTPRGQQPGGATK
                     MLFSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQL
                     VSLPDFSHYELYDKPEAVQEALEKVIPFFNTHLG"
     gene            3093..3731
                     /locus_tag="RH057_000551"
     CDS             3093..3731
                     /locus_tag="RH057_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3126967.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSKIIMGDVLNHAALKQAMQGQDVVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFGEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            4022..4750
                     /gene="mpaA"
                     /locus_tag="RH057_000552"
     CDS             4022..4750
                     /gene="mpaA"
                     /locus_tag="RH057_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415842.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HDV3126968.1"
                     /translation="MTVTRPRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHSELGEWLSQAFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(4725..5690)
                     /gene="ycjG"
                     /locus_tag="RH057_000553"
     CDS             complement(4725..5690)
                     /gene="ycjG"
                     /locus_tag="RH057_000553"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HDV3126969.1"
                     /translation="MRTVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPGTVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFRLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            5809..6315
                     /gene="tpx"
                     /locus_tag="RH057_000554"
     CDS             5809..6315
                     /gene="tpx"
                     /locus_tag="RH057_000554"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HDV3126970.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(6359..7900)
                     /gene="tyrR"
                     /locus_tag="RH057_000555"
     CDS             complement(6359..7900)
                     /gene="tyrR"
                     /locus_tag="RH057_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HDV3126971.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGMFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(8048..9109)
                     /locus_tag="RH057_000556"
     CDS             complement(8048..9109)
                     /locus_tag="RH057_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HDV3126972.1"
                     /translation="MTEPLKPRIDFDGPLEVDQNPKFRAQQTFDENQAQNFAPATLDE
                     APEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGVQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTARLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(9106..10503)
                     /locus_tag="RH057_000557"
     CDS             complement(9106..10503)
                     /locus_tag="RH057_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HDV3126973.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDSLLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSVKWRMMCEGLDPLAPADENRLADIAAAWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWGESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AATKADHVTIDQHANMVSLLQQLIQDAWQNAAFEGISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGAPLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            10658..11656
                     /locus_tag="RH057_000558"
     CDS             10658..11656
                     /locus_tag="RH057_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3126974.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVIRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLAAHNIAVRD
                     EWIVDGGYTHETALQAARELLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDIPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(11767..12672)
                     /gene="ompG"
                     /locus_tag="RH057_000559"
     CDS             complement(11767..12672)
                     /gene="ompG"
                     /locus_tag="RH057_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
                     /protein_id="HDV3126975.1"
                     /translation="MKKLLPCTALVMCAGMACAQAEERNDWHFNIGAMYEIENVEGYG
                     EDMDGLAEPSVYFNAANGPWRIALAYYQEGPVDYSAGKRGTWFDRPELEVHYQFLEND
                     DFSFGLTGGFRNYGYHYVDEPGKDTANMQRWKIAPDWDVKLTDDLRFNGWLSMYKFAN
                     DLNTTGYADTRVETETGLQYTFNETVALRVNYYLERGFNMDDSRNNGEFSTQEIRAYL
                     PLTLGNHSVTPYTRIGLDRWSNWDWQDDIEREGHDFNRVGLFYGYDFQNGLSVSLEYA
                     FEWQDHDEGDSDKFHYAGVGVNYSF"
     gene            complement(12717..13799)
                     /locus_tag="RH057_000560"
     CDS             complement(12717..13799)
                     /locus_tag="RH057_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3126976.1"
                     /translation="MAQLSLQHIQKIYDNQVHVVKDFNLEIADKEFIVFVGPSGCGKS
                     TTLRMIAGLEEISGGDLLIDGKRMNDVPAKARNIAMVFQNYALYPHMTVYDNMAFGLK
                     MQKIAKEVIDERVNWAAQILGLREYLKRKPGALSGGQRQRVALGRAIVREAGVFLMDE
                     PLSNLDAKLRVQMRAEISKLHQKLNTTMIYVTHDQTEAMTMATRIVIMKDGIVQQVGA
                     PKTVYNQPANMFVAGFIGSPAMNFIRGTIDGDKFVTETLKLTIPEEKLAVLKTQGSLH
                     KPIVMGIRPEDIHPDAQEENNISAKISVAELTGAEFMLYTTVGGHELVVRAGALNDYH
                     AGENITIHFDMTKCHFFDAETEIAIR"
     gene            complement(13813..14472)
                     /gene="pgmB"
                     /locus_tag="RH057_000561"
     CDS             complement(13813..14472)
                     /gene="pgmB"
                     /locus_tag="RH057_000561"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HDV3126977.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNSQERAQLAYRKNLLYVHSLRELTVSAVLPGIRSLL
                     ADLRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(14469..16736)
                     /gene="ycjT"
                     /locus_tag="RH057_000562"
     CDS             complement(14469..16736)
                     /gene="ycjT"
                     /locus_tag="RH057_000562"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HDV3126978.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELMNLPDVVGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGTGYTIASRRFVSADQLPLIVLEITITPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCMVSGDVQQCYTAKERRLQQHTS
                     AQLHAGETVTLQKLVWIDWRDDRQAVLDEWGSASLRQLEMCAQQSYDQLLAVSTENWR
                     QWWQKRRITVNGGEAHDQQALDYALYHLRIMTPAHDERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMAYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMAKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSAASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LPEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLNGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(16733..17788)
                     /locus_tag="RH057_000563"
     CDS             complement(16733..17788)
                     /locus_tag="RH057_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3126979.1"
                     /translation="MKSAMTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSR
                     LSQAQALAEKYGNASVWDDPQAMLLAVKPDVVSVCSPNRFHYEHTLMALEAGCHVMCE
                     KPPAMTPEQAREMCDTARKLGKVLAYDFHHRFALDTQQLRDQVTNGVLGEIYVTTARA
                     LRRCGVPGWGVFTNKELQGGGPLIDIGIHMLDAAMYVLGFPAVKSVNAHSFQKIGTQK
                     SCGQFGEWDPATYSVEDSLFGTIEFHNGGILWLETSFALNIREQSIMNVSFCGDKAGA
                     TLFPAHIYTDNNGELMTLMQREMADDNRHLRSMEAFINHVQGKPVMIADAEQGYIIQQ
                     LVAALYQSAETGTRVEL"
     gene            complement(17798..18586)
                     /locus_tag="RH057_000564"
     CDS             complement(17798..18586)
                     /locus_tag="RH057_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HDV3126980.1"
                     /translation="MKIGTQNQAFFPENILEKFRYIKEMGFDGFEIDGKLLVNNLEEV
                     KAAIKETGLPVTTACGGYDGWIGDFIEERRLNGLKQIERILEALAEVGGKGIVVPAAW
                     GMFTFRLPPMTSPRSLDGDRKMVSDSLRVLEQVAARTGTVVYLEPLNRYQDHMINTLA
                     DARRYIVENDLKHVQIIGDFYHMNIEEDNLAQALHDNRDLLGHVHIADNHRYQPGSGT
                     LDFHALFEQLRADNYQGYVVYEGRIRAEDPAQAYRDSLAWLRTC"
     gene            complement(18604..19656)
                     /locus_tag="RH057_000565"
     CDS             complement(18604..19656)
                     /locus_tag="RH057_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HDV3126981.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAYRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(19687..20529)
                     /locus_tag="RH057_000566"
     CDS             complement(19687..20529)
                     /locus_tag="RH057_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HDV3126982.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKGAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSLVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALSERFIKSGLTAG
                     GVKG"
     gene            complement(20516..21397)
                     /locus_tag="RH057_000567"
     CDS             complement(20516..21397)
                     /locus_tag="RH057_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HDV3126983.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     HLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(21418..22710)
                     /locus_tag="RH057_000568"
     CDS             complement(21418..22710)
                     /locus_tag="RH057_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3126984.1"
                     /translation="MIKSKIVLLSALVSCALISGCKEENKTNVSIEFMHSSVEQERQA
                     VISKLIARFEKENPGITVKQVPVEEDAYNTKVITLSRSGSLPEVIETSHDYAKVMDKE
                     QLIDRKAVATVISNVGEGAFYDGVLRIVRTEDGSAWTGVPVSAWIGGIWYRKDVLAKA
                     GLEEPKNWQQLLDVAQKLNDPANKKYGIALPTAESVLTEQSFSQFALSNQANVFNAEG
                     KITLDTPEMMQALTYYRDLAANTMPGSNDIMEVKDAFMNGTAPMAIYSTYILPAVIKE
                     GDPKNVGFVVPTEKNSAVYGMLTSLTITAGQKTEETEAAEKFVTFMEQADNIADWVMM
                     SPGAALPVNKAVVTTATWKDNDVIKALGELPNQLIGELPNIQVFGAVGDKNFTRMGDV
                     TGSGVVSSMVHNVTVGKADLPGTLQASQKKLDELIEQH"
     gene            complement(22724..24403)
                     /gene="ycjM"
                     /locus_tag="RH057_000569"
     CDS             complement(22724..24403)
                     /gene="ycjM"
                     /locus_tag="RH057_000569"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HDV3126985.1"
                     /translation="MKQKITDYLDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPTFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YQQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQHPGFEDFFIAVDP
                     QTDLSAVTRPRALPLLTPFQMRDHSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LAKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDNVAPGTVIITETNVPH
                     KDNIAYFGAGDDEAHMVYQFSLPPLVLHAVQKQNVEALCAWAQNLTLPSSNTTWFNFL
                     ASHDGIGLNPLRGLLPESEILELVEALQQEGALVNWKNNPDGTRSPYEINVTYMDALS
                     RRESSDEERCARFILAHAILLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKY
                     HSKEITRELNDEATLRHAVYHELSRLITLRRSHNEFHPDNNFTIDTINSSVMRIQRSN
                     ADGNCLTGLFNVSKNIQHVNITNLHGRDLISEVDILGNEITLRPWQVMWIK"
     gene            complement(24615..24929)
                     /gene="pspE"
                     /locus_tag="RH057_000570"
     CDS             complement(24615..24929)
                     /gene="pspE"
                     /locus_tag="RH057_000570"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HDV3126986.1"
                     /translation="MFKKGLLALALVFSLPVFAAEHWIDVRVPEQYQQEHVQGAINIP
                     LKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKEILSEMGYTHVENAGGLKDIAMPKV
                     KG"
     gene            complement(25004..25225)
                     /gene="pspD"
                     /locus_tag="RH057_000571"
     CDS             complement(25004..25225)
                     /gene="pspD"
                     /locus_tag="RH057_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HDV3126987.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(25234..25593)
                     /gene="pspC"
                     /locus_tag="RH057_000572"
     CDS             complement(25234..25593)
                     /gene="pspC"
                     /locus_tag="RH057_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HDV3126988.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(25593..25817)
                     /gene="pspB"
                     /locus_tag="RH057_000573"
     CDS             complement(25593..25817)
                     /gene="pspB"
                     /locus_tag="RH057_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HDV3126989.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLADEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(25871..26539)
                     /gene="pspA"
                     /locus_tag="RH057_000574"
     CDS             complement(25871..26539)
                     /gene="pspA"
                     /locus_tag="RH057_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HDV3126990.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            26706..27683
                     /gene="pspF"
                     /locus_tag="RH057_000575"
     CDS             26706..27683
                     /gene="pspF"
                     /locus_tag="RH057_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HDV3126991.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEDAIAVSETTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(27803..29068)
                     /gene="puuE"
                     /locus_tag="RH057_000576"
     CDS             complement(27803..29068)
                     /gene="puuE"
                     /locus_tag="RH057_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HDV3126992.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINALAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGEGGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(29106..30386)
                     /gene="puuB"
                     /locus_tag="RH057_000577"
     CDS             complement(29106..30386)
                     /gene="puuB"
                     /locus_tag="RH057_000577"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HDV3126993.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWERYGNKQLELLDANAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTAKGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARDPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSRMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(30388..31875)
                     /gene="puuC"
                     /locus_tag="RH057_000578"
     CDS             complement(30388..31875)
                     /gene="puuC"
                     /locus_tag="RH057_000578"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HDV3126994.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVVLKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANLV
                     FADCPDLQKAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAELAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(32150..32707)
                     /gene="puuR"
                     /locus_tag="RH057_000579"
     CDS             complement(32150..32707)
                     /gene="puuR"
                     /locus_tag="RH057_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HDV3126995.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(32734..33498)
                     /gene="puuD"
                     /locus_tag="RH057_000580"
     CDS             complement(32734..33498)
                     /gene="puuD"
                     /locus_tag="RH057_000580"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HDV3126996.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPGSPSNVQPHLYGENGDEPDADPGRDLLSMALINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITACQHHIAEKQRL"
     gene            33710..35128
                     /gene="puuA"
                     /locus_tag="RH057_000581"
     CDS             33710..35128
                     /gene="puuA"
                     /locus_tag="RH057_000581"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HDV3126997.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGYLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYRVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            35277..35441
                     /locus_tag="RH057_000582"
     CDS             35277..35441
                     /locus_tag="RH057_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3126998.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRLTRPQTL
                     LRRGGSNYGY"
     gene            35431..36816
                     /gene="puuP"
                     /locus_tag="RH057_000583"
     CDS             35431..36816
                     /gene="puuP"
                     /locus_tag="RH057_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HDV3126999.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            36950..37195
                     /locus_tag="RH057_000584"
     CDS             36950..37195
                     /locus_tag="RH057_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HDV3127000.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            37508..39151
                     /gene="sapA"
                     /locus_tag="RH057_000585"
     CDS             37508..39151
                     /gene="sapA"
                     /locus_tag="RH057_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HDV3127001.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKSLGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCDPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            39148..40113
                     /gene="sapB"
                     /locus_tag="RH057_000586"
     CDS             39148..40113
                     /gene="sapB"
                     /locus_tag="RH057_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HDV3127002.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKEVFPATMELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            40100..40990
                     /gene="sapC"
                     /locus_tag="RH057_000587"
     CDS             40100..40990
                     /gene="sapC"
                     /locus_tag="RH057_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HDV3127003.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            40990..41982
                     /gene="sapD"
                     /locus_tag="RH057_000588"
     CDS             40990..41982
                     /gene="sapD"
                     /locus_tag="RH057_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HDV3127004.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            41984..42790
                     /gene="sapF"
                     /locus_tag="RH057_000589"
     CDS             41984..42790
                     /gene="sapF"
                     /locus_tag="RH057_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HDV3127005.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            42858..43211
                     /locus_tag="RH057_000590"
     CDS             42858..43211
                     /locus_tag="RH057_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HDV3127006.1"
                     /translation="MMDKIKSNARDLRSNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDSRRTSWLESQGWTVLRFWNNEIDCNEETV
                     LENILQELNRRSPSP"
     gene            43581..44369
                     /gene="fabI"
                     /locus_tag="RH057_000591"
     CDS             43581..44369
                     /gene="fabI"
                     /locus_tag="RH057_000591"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HDV3127007.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            44514..45641
                     /locus_tag="RH057_000592"
     CDS             44514..45641
                     /locus_tag="RH057_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309887.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HDV3127008.1"
                     /translation="MEQRHITGKSHWYHETQSSTAEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTAGDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLEIQNYADASLFADESIRWRSSYEV
                     EKLPEEYTKSSTAELCQLAETLSLHPISLSLLERLLNSTRVNTQPDNKLAALLCARIN
                     GSPACFAACMDSSNEYKKISPLLRKGENEINQWADRHSVEHATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            45709..47643
                     /gene="rnb"
                     /locus_tag="RH057_000593"
     CDS             45709..47643
                     /gene="rnb"
                     /locus_tag="RH057_000593"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HDV3127009.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLAIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTALDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWQPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISTEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTDTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAMRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(47721..47825)
                     /locus_tag="RH057_000594"
     CDS             complement(47721..47825)
                     /locus_tag="RH057_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127010.1"
                     /translation="MTYMKLTAAKRIVFFIYLFVIQFWKNASFQTKVS"
     gene            47879..49864
                     /gene="pdeR"
                     /locus_tag="RH057_000595"
     CDS             47879..49864
                     /gene="pdeR"
                     /locus_tag="RH057_000595"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV3127011.1"
                     /translation="MKTVRESTTLYNFLGSHNPYWRLTESSDVLRFSTTETTEPDRTL
                     QLSAEQAARIREMTVITSSLMMSLTVDESDLSVHLVGRKINKREWAGNASAWHDTPAV
                     ARDLSHGLSFAEQVVSEAHSAIVILDSRGNIQRFNRLCEDYTGLKEHDVIGQSVFKLF
                     MSRREAAASRRNNRVFFRSGNAYEVELWIPTRKGQRLFLFRNKFVHSGSGKNEIFLIC
                     SGTDITEERRAQERLRILANTDSITGLPNRNAMQDLIDHAINHADNNKVGVVYLDLDN
                     FKKVNDAYGHLFGDQLLRDVSLAILSCLEHDQVLARPGGDEFLVLASNTSQSALEAMA
                     SRILTRLRLPFRIGLIEVYTSCSVGIALSPEHGSDSTAIIRHADTAMYTAKEGGRGQF
                     CVFTPEMNQRVFEYLWLDTNLRKALENDQLVIHYQPKITWRGEVRSLEALVRWQSPER
                     GLIPPLDFISYAEESGLIVPLGRWVILDVVRQVAKWRDKGINLRVAVNISARQLADQT
                     IFTALKQVLQELNFEYCPIDVELTESCLIENDELALSVIQQFSQLGAQVHLDDFGTGY
                     SSLSQLARFPIDAIKLDQVFVRDIHKQPVSQSLVRAIVAVAQALNLQVIAEGVESAKE
                     DAFLTKNGINERQGFLFAKPMPAVAFERWYKRYLKRA"
     gene            50011..50184
                     /locus_tag="RH057_000596"
     CDS             50011..50184
                     /locus_tag="RH057_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127012.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKAPWEI"
     gene            50274..51023
                     /locus_tag="RH057_000597"
     CDS             50274..51023
                     /locus_tag="RH057_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3127013.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVYF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            51292..51510
                     /gene="osmB"
                     /locus_tag="RH057_000598"
     CDS             51292..51510
                     /gene="osmB"
                     /locus_tag="RH057_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HDV3127014.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(51636..51962)
                     /gene="yciH"
                     /locus_tag="RH057_000599"
     CDS             complement(51636..51962)
                     /gene="yciH"
                     /locus_tag="RH057_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HDV3127015.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGVVRIQRQTSGRKG
                     KGVCLITGVDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(51962..52699)
                     /gene="pyrF"
                     /locus_tag="RH057_000600"
     CDS             complement(51962..52699)
                     /gene="pyrF"
                     /locus_tag="RH057_000600"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HDV3127016.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNRDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLVDLGMTLSPADYAERLAALTQK
                     CGLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALSAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(52892..54061)
                     /gene="lapB"
                     /locus_tag="RH057_000601"
     CDS             complement(52892..54061)
                     /gene="lapB"
                     /locus_tag="RH057_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HDV3127017.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYRCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(54068..54376)
                     /gene="lapA"
                     /locus_tag="RH057_000602"
     CDS             complement(54068..54376)
                     /gene="lapA"
                     /locus_tag="RH057_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HDV3127018.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(54525..55289)
                     /gene="pgpB"
                     /locus_tag="RH057_000603"
     CDS             complement(54525..55289)
                     /gene="pgpB"
                     /locus_tag="RH057_000603"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HDV3127019.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            55459..56049
                     /gene="ribA"
                     /locus_tag="RH057_000604"
     CDS             55459..56049
                     /gene="ribA"
                     /locus_tag="RH057_000604"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HDV3127020.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(56113..58788)
                     /gene="acnA"
                     /locus_tag="RH057_000605"
     CDS             complement(56113..58788)
                     /gene="acnA"
                     /locus_tag="RH057_000605"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HDV3127021.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSTLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELEVNATHKDRQPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLAAEPIGHDRKGDPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLELTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(58952..59047)
                     /locus_tag="RH057_000606"
     CDS             complement(58952..59047)
                     /locus_tag="RH057_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127022.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(59161..59328)
                     /locus_tag="RH057_000607"
     CDS             complement(59161..59328)
                     /locus_tag="RH057_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127023.1"
                     /translation="MVGQEQLESSPLCQHSDNETETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(59331..59495)
                     /locus_tag="RH057_000608"
     CDS             complement(59331..59495)
                     /locus_tag="RH057_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HDV3127024.1"
                     /translation="MLISDSDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
BASE COUNT        14421 a        15736 c        14788 g        14755 t
ORIGIN      
        1 gccaaaatgc ggcaaagcac atagaacacc gaacactacg ttcaggcttc gcacgttcaa
       61 accagttttc ggtatcaaaa tcagcatctg tcgccattgt acgaagaata aaaaataacc
      121 aaaacgttga aaggaactga gaatacgtta agagagaaat taaattaatg tatcagcaag
      181 tagtgctaaa cagcaccttt atacttcagc ctatctataa ataatgagaa tgccgagcca
      241 gcatgtcgac ggtgtggata gtacaggtga taaccgggta aatctggtgt gaatttatcc
      301 aaaacacgta tcagtttttt ttctttaata gcccgctcaa cctgatcata aggtagatac
      361 gccaatccat gcccatcaat tgcagcatca atgatcaggt ctatcgtatt cagtaaaagc
      421 tgaccttcca tgcgaacacg aacttcacgt ccaccgcgta ttaatctcca gcgatttgct
      481 gtacccgatg tgggaagata caaattaatt gcctgatgat ctattaattg tgacactgac
      541 gttggaacac ttcggcgaga aaaataatcc ggtgagccaa caatagccat tggtatatct
      601 ggcccgattc gaatggcgat catatcttta tccatctccc cacccagacg gacgcctgca
      661 tcaaaacgtt cagaaacgac atcggtcaaa ccataatcaa tggtgagctg aatatcaatt
      721 tcaggatgcg atttcaggaa tgtgcgcatt gctggtaaca atatcgtttt tgctgcatgt
      781 tctacagtag taatacgtat tgtcccggat gggcggttct gcagctcgct cagggatgcc
      841 agggctgaat ctatgtcatg caacataggg ccaagaacag acaaaagatg ctcgcccgct
      901 tcagttggaa caacgctgcg cgtggttcgc gtcagaagcc gcaatcccaa tcgttcttct
      961 atacgacgca ctatctggct taaagctgac tgcgccatgc tcaggcgggc tgctgcacga
     1021 gtgaagctac gctcctctgc aacgacgaca aacgccatca gatcagcgat ttcttcacgc
     1081 ttcattcagt atctccatgc aatctattaa tcatttaaat gataggtcta atcatgattt
     1141 tgggtctaat caacttaatg atcaatatct atagtgatat caccaaccgt taaaacgaac
     1201 cgacaactcc atgaggaacg gaatgatgaa taataaagtc agcttcacta acagcaataa
     1261 tccaaccatc tctttgtctg cagtgatcta tttccctcca aaatttgatg aaacccgtca
     1321 gtatcaggca attgtggtat ctcacccagg tgggggcgtt aaagaacaaa cggccggaac
     1381 ctatgccaaa aaactggcgg aaaagggatt tgtcactatt gcttatgacg catcttatca
     1441 aggtgaaagt ggcggcgagc cgcgtcagtt agaaaaccca tacattcgta ctgaagacat
     1501 cagtgccgtg attgattacc tgacaacgct ttcttatgtc gacaataccc gaattggtgc
     1561 gatgggaatt tgtgccggtg caggatatac agccaatgct gcaattcagg atcgccgcat
     1621 caaggccatc ggtaccgtta gtgcggtcaa tattggttcg atgttccgta atggttggga
     1681 aaacaatgtg aaatctattg atgccctgcc atacgttgaa gcgggttcaa acgcccgaac
     1741 cagcgatatt agcagcggag aatatgccgt tatgccactg gcaccaatga aggagtccga
     1801 tgcgccgaac gaagaactgc gtcaggcctg ggagtattac cacacccctc gcgcgcagta
     1861 tccaacagca ccgggttacg ctactttgcg cagccttaac cagattatta cctatgatgc
     1921 ttaccatatg gcggaagtgt acctgactca gccgatgcag attgtggcgg gcagccaggc
     1981 agggagcaaa tggatgagtg acgacctgta cgatcgggca tcaagccagg ataaacgcta
     2041 tcacattgtt gaaggcgcaa accatatgga tctttatgac ggcaaagcct atgtggctga
     2101 agcaatttca gtattagctc cgttctttga ggaaactctg taatgaaaca tattcaaatc
     2161 cgcaatagtg atatggcctg gcatattgct gccaatattc agtttccacc aaattttgat
     2221 gagtcaaaac agtatccggc gattatcagc gtccatcctt ttggcagttg taaggagcag
     2281 acgtctggca atatctatgg caaagcattg gctgaaaaag gctatctcgt tctcgcttat
     2341 gacgccagtt tccagggaga atctggtggg gagcctcgct ggatagaaga tccgacacag
     2401 cgcgtagagg atattagccg tgttatcgat tatgcagtaa ctctgccata tgttaatgct
     2461 gaacgtattg gcgtgctggg agtgtgtggg ggtggtggct atgccattaa tgccgcgctg
     2521 acagaaaaac gaattaaagc agtagtgagt attacgggtg ttaatatcgg gcgtcttttt
     2581 cgggaaggtt tcagcaatta cgatccgatt ggcgcattga atgcaatggc gtcgcaacga
     2641 accaaagagg ctcgtggtgg agaattacag atcaacgaat tacttcccgc cagcctggat
     2701 gcagcaaaat cccatggact gaccgaacgc gatgtttatg aagcaactga ctactataaa
     2761 acaccacgcg ggcaacaacc gggaggtgcg acaaaaatgt tgttctcaca tgcacagaaa
     2821 acgctggcct gggatgcctt cgcatttact gaagtgttac tgactcagcc ggtgatggta
     2881 gtagttggtg aaaaagtcgg tgccttcggt gcttatcgtg atggactgga agtctatggc
     2941 cgggcgatgg tttcgcagga tcgtcagtta gtttcattac ccgatttctc acattatgaa
     3001 ctgtatgaca aaccggaagc agttcaggag gcgctggaaa aagttattcc ttttttcaac
     3061 acccatctgg gataagtttc atgagaaata ttatgaaaaa tgtactgatt cttggtgctg
     3121 gcggtcagat agcccgccat gtgattaatc aactggcaga taaacaaacc attaaacaaa
     3181 ctttatttgc ccgtcagcca gcaaaaatcc acaaacccta tccaactaat agcaagatta
     3241 ttatgggaga tgtactgaat catgcagcac tgaagcaggc catgcaggga caggacgttg
     3301 tgtatgccaa ccttacgggg gaagatttag atattcaggc aaattcagtg atcgctgcga
     3361 tgaaagcctg tgatgttaaa cgtctgatct ttgttctttc cctcggtatt tatgatgaag
     3421 ttccaggaaa atttggcgaa tggaataatg cagttattgg tgagccccta aaaccattcc
     3481 gcagggctgc ggatgcaatt gaggcttctg gactggaata tacaattctt cgccctgcct
     3541 ggctgactga tgaagatatt attgattatg agcttacctc acgcaatgaa ccattcaaag
     3601 gtacgatcgt atcacgtaag agtgtcgcag cattaatcac agatatcatc gataaacctg
     3661 agaaacacat tggtgagaat ataggcatca atcaacccgg tactgatggt gataagccat
     3721 tcttcatgta aatcaattac ataactacca tcgtactgta ctatgtgcag tacgatttat
     3781 taaacttatt aaggtgaact gatcaatccg atgcgattaa catgtgtaga accagtaaga
     3841 ataaaatacg ccatgtatat aacattacga caattctaat atctgcacat caatgaaaat
     3901 gcaaacaact tccggcaaaa aagtgataca agatcgatga catagcgttg aagaaaagga
     3961 tttgataatc cgcaaaattt gcacaacact gcctgtaata cttcctgaac cagagacgat
     4021 catgaccgta acccgcccac gcgccgaacg cggcgcattt ccgcccggaa cagaacatta
     4081 cggacgttca ttattgggcg cgccgttaat ctggtttcct gcccctgcag ccagtcgcga
     4141 aagtggtttg attctggctg gcactcacgg tgatgaaaac tcttcggtcg tcacgctctc
     4201 ctgtgctctg cggactttga caccttcttt gcgccgccat catgtggtgc tgtgtgtgaa
     4261 tcctgacggc tgccagttag ggttacgggc caatgctaat ggtgtggatt taaacagaaa
     4321 ctttccggcg gcgaactgga aggaaggtga aacggtttat cgctggaaca gcgccgctga
     4381 agaacgtgat gtcgtattac tgacaggtga caaacctggc tccgaacctg aaacccaggc
     4441 gctgtgccag cttatacacc gcattcaacc tgcctgggtg gtctcattcc acgatccact
     4501 ggcttgtatt gaagatccca gacacagcga attaggcgaa tggctgtccc aggcgtttga
     4561 attgcctctg gtaaccagcg tcggctatga aacgcctggc tctttcggca gttggtgtgc
     4621 agatctcaac ttgcattgca tcaccgcaga gtttccgccc atctcctccg acgaagccag
     4681 cgaaaaatat ctctttgcga tggcaaactt gctgcgctgg catcctaaag atgcaattcg
     4741 cccgtcgtga actgaagcgc cggttccaca tctaccgcca gccaggtcgg tccgtcaaga
     4801 tcggcgaaac tgacctgcgg caccagcggt aaagcggcgc taatggcacg agaggtacac
     4861 aacatgcagc ccagcatcag acgaaaacct tgtgcacgtg cttcagtcgc cagcgccagc
     4921 gcttccgtca gacccccggt tttatcgagc ttaatgttaa ccatctcata gcgccctttc
     4981 agcgccttca gattgctacg agtatgacaa ctttcatcag cacaaatcgg caacggatga
     5041 ataaaattct ccagtgccgc atcgtcctgc gcaggaagcg gttgttcaag catcgcaacg
     5101 cctaaatccg ccaatagctg gcaacgcgcc gccaaccctt ctgcacgcca ggattcattt
     5161 gcatcaacga tcagcgtggc atcgggcaca gcggtgcgaa ttgccaccat ccgctcactg
     5221 ataagatggt tatccagctt cactttcagt aatttcgcgc ctgcctgcca gagtgttgat
     5281 gcactattgg ccatctgatc aggcgtaccg atgacaaccg tctgtgcagt gataactgtc
     5341 ccaggaagcg ttatgccgat caaatcagcc agcgattgct gctgttttcg cgccgccaga
     5401 tcccacaatg cacaatccag cgcattacgt gctgcgccgg cagggagaat tttttgcaac
     5461 tcctcccgtg tcagcccttt ctctaattgc ggtacgacgc tcataatttg cgccattacc
     5521 gaggcatcac tttccccata acgcggatac ggcgtgcatt cgccggtgcc tttaataccc
     5581 tcttcttcca gttcaaccac caccacgcgc gcttcactgc gacttccccg ggcaatcaca
     5641 aacggggtat gtaagggcca ggcttcctca aataccttaa cggttctcat ctttactcct
     5701 tgttgtcgcg gtggtctgcg gattaagcgc aaatagcgtt tgctgtgtta ttgacagtta
     5761 gcataaacta ggtgtgacgt taactatatg taaacaggaa agataatcat gtcacaaacc
     5821 gttcatttcc agggcaaccc ggttacagtc gccaattcca tcccgcaggc gggtagcaaa
     5881 gcgcagactt ttactctcgt ggcaaaagat ctgtctgacg tcaccctcgg tcagtttgcg
     5941 ggtaaacgca aagtgctgaa cattttcccg agtattgata ccggagtttg cgccgcatca
     6001 gtacgtaagt ttaaccaact ggcaaccgag atcgacaaca ccgttgtgct gtgtatttct
     6061 gccgatctgc cgttcgccca gtctcgtttc tgcggcgcag aaggtctgaa caacgttatc
     6121 accctctcca ctttccgtaa cgctgaattc ctgcaagcct acggtgtggc aattgctgat
     6181 ggcccactga aaggtctggc agcacgtgcc gttgtggtta ttgacgaaaa tgacaatgtg
     6241 attttcagcc agctggtgga tgaaatcacc accgagccgg attacgaagc agctctggct
     6301 gtactgaaag cataatttaa tatgcctgat ggtgttgcac catcaggcat attcgcgctt
     6361 actcttcgtt cttcttctga ctcagaccat attcccgcaa cttattggca atcgcggtat
     6421 gtgaaacgcc gagacgtttt gccagtttgc gcgtgctggg ataattgcga taaagctggg
     6481 ttaataccga gcgttcaaaa cggctggtga tttcgtccag cgaaccttcc atcgcatctt
     6541 cgcccacggc taccgttgcg gcgtcataat ccggcaacaa aatatcctgt ggacgcagct
     6601 cataaccgtc cagttgtgtc agtgcgcgat agatagcgtt ctttaactgc cgcacatttc
     6661 ccggccacgc ataacgcgta agtacagtat tcaggtcagc ggccagtttc ggacgcggca
     6721 cgccctgctc gtcggcaaag cgggcgacga acagctcagt taacggcatg atgtcctgcg
     6781 gacagtcacg tagcggcggc agattgagcg tcaacacgtt cagacgataa tagagatctt
     6841 cacggaacat gcctttttgc accagttcga ccagattctt ctgcgtagcg caaatcaccc
     6901 gcacatcgac atgcacctca tggtcttcgc caacccgacg gaaagtgcca tcattaagga
     6961 aacgcagtaa tttcgcctgc atccgtggtg acatctcacc tatttcatcc aacagcaccg
     7021 aaccaccgtt cgcctgctca aagaatcctt tcttcccttc cggagcatga ccaaacagtt
     7081 cactctcgac cgcatcttct ggtatagacg cgcaattcag cgccaggtaa ggtttgcccg
     7141 ctctggggct tgcctgatgg caggcgtagg caaaaagatc tttacctgta cctgtgtcac
     7201 ccgtaatcag cagcggcgcg cttagcatcg ccagtttctg cgcctgttcg acaacatgct
     7261 tcattttcgg gctgacggcg acaatttgac taaaggcgct gacgtcctgg gcggcaacat
     7321 tttgcaactg acggcccata cgaatcgttg atcgcaacat caccaccgca ccggtcagga
     7381 cgtgttgatc attttcatcc tgaagataaa caggcgtaat ctccatcagg aaattctgcc
     7441 cattaataac aacatgctcg ttatgcgaat cttgcggttc gctttccagc caacgtaaaa
     7501 aattaaagcc gttaatcaat tgtgcggcgg tatggttgcg caggcgatcc aatttttgcc
     7561 caaaaagctg acagctcgcc gggtttgcca tatccacttt gcttttcata tcgacagaga
     7621 gcacaggttc aggcaacgcc tccagtaacg cgctcaacgc cagatgctca cgttcggaag
     7681 gcatccacgg gacagtacgc acatcggtaa cacctgcaat acggcgtatt tcggccatca
     7741 gactgctgaa actctcaaac tccagttcag caaaattgag gtagattcgc ccaatggggt
     7801 caatctcaat accgcgtaaa tcaatgcctc ttagcacgag tagatcgagt aattcgcggg
     7861 tcagaccgag tcggtcttca caaaagactt ccagacgcat gggaaccttc acctgaaaaa
     7921 agaacaatta atatgatgat atgacactat ttggatagca ggaaggtttc tgtcaacaat
     7981 cattgacaca aagacggaaa aacgggcact tcggcgtaaa gatatccggt cagcacccga
     8041 tattgcatta tttttcgctg ggcgttttgc ctttttgcag cgtttctttc acctgaccga
     8101 taagctgacg acggaaatcc ccgaggcgag gtttgtcatc gtcaatccac ggcagcgggc
     8161 ggcaaagctc catagcttta atcccgagtc gtgccgtcag aagtccagca ccaattccct
     8221 gagctgcgcg ggtagacaaa cgagcagcga gatcttgcga catccagtcc atccccactt
     8281 cgcgcaccag ttcgctggct ccggcaaaag cgatattcag caataccagc ttaaacagac
     8341 gcaaacggct gtaatacccc agttcaatgc catacagcgt ggcgatgcga ttaattaaac
     8401 gcagattgcg ccaggcgata aacgccatat cgaccaacgc cagcgggctg accgcaatca
     8461 tcaacgttga ttccgccgcc gaacggctga tttcgcgccg cgcctgggca tctaaaactg
     8521 gctggaccaa atgcgcatac aaactgacca cttcacggtc gttttgcgtt tcatggattg
     8581 aggcatacca gcgttgcagc gctggatgag actgatcaat acccgcctgc tgcgccagtt
     8641 tttcgcaaaa tgcgcggcct ttgcccgtgc cgtggctgtg caacaaatcg cgcgcttcgt
     8701 cgcgttcatg ggcgcgctgt cgcaagcgcc ataagcgccg ccactctgtt accacagaac
     8761 ctacgccagc gccgataatc aatgccccag cggcacatcc acccagcgcc acccagtcct
     8821 gagtttgcca ggcattcatt gtccactgta caccctgccc gacaacgctt gcgccaaaca
     8881 gagccagccc gcccatcacc attttgcgcc acaggctgcg tttcggacgt aacgctgcat
     8941 ccattaccgc ttcaacttgc ccctcttctt caggcgcttc atcgagcgtg gccggggcaa
     9001 aattttgcgc ctgattttcg tcaaaggtct gctgcgccct gaattttgga ttctgatcga
     9061 cctccagcgg accgtcgaaa tcaatacgtg gttttaacgg ttcggtcatc gcaatttatc
     9121 tcctattaaa aattcaagcg cggcatcaag gcgaatatgc ggcagcggtt tgtcgacatc
     9181 catcacctgc ggacgaaacg cctcaaactg gaagccttgc ttatcccaga acgcctgacc
     9241 gggcaaacga gcgggaactt cgccaggata aacagtgagc ggtgcgccat cgctgagtcg
     9301 attaccgcgt agcgccggga ttttctcacc gttaacatca ataatgccgc tggtggtcgc
     9361 ctgcaccgag gccagcccca ggcaatccat actaatccct tcaaacgccg cattttgcca
     9421 ggcatcctga atcagttgtt gcagtaatga aaccatattg gcgtgctgat cgatggtcac
     9481 atggtccgct ttagtggcag caaacaatag cttatcgata accggcgaaa acaaacgccg
     9541 gaacaaggta cgctgcccgt agtggaaact ttgcatcagc tgcgtcagtg ccagacgcat
     9601 atcattaaat gcctgtggcc cactgttgag aggttgcagg caatcaacca gcacaatctg
     9661 gcggtcaaag cgcagaaaat gattcttata gaaccccttc accaccttct cgcagtaata
     9721 attaaaccgc tcgcgcagca ttccggcatt ggtgtgctta tcggcctgcg ccagtttgga
     9781 ctcgccccag gcatcgacat ccggccacgg gaagaattgc agcgcgggcg cacctgccat
     9841 atctcccggc aagacaaagc gcccaggctg aataaagtgc agcccctgct gtttacagtg
     9901 gtggagataa tcggtccacg cggcggcaat gtctgccagc cggttttcgt cggcaggcgc
     9961 tagcgggtcc agcccttcgc acatcattcg ccatttgacc gaccattcgc cgcgctgacc
    10021 attgagtaag cccgtcatct ggcgtgacca gcttaaatag tcctgcgcca gcatcggcag
    10081 gtcgagcaac cattcgccgg gataatccac aatctccaga tacagcgtgg aggtgtcctt
    10141 gaagtggcgt agcagcgaat cgttcgattt aaaacgtagc gccaggcgga tttcactgac
    10201 gccgcgcgtt ggcgtcggcc aggcgggagg atcgccatat aactgcgcca gtccttcatc
    10261 atatgtgaag cgcggaatgc caaagtcacg ctgaggaatg cgttttacgc ccagcaggcg
    10321 ctcttcacgc accgcgctta acagcggcaa gcgtgctccg gcgtgaatat tgagcaactg
    10381 attgaccatc gcagtgataa acgctgtttt gccgctgcgg cttagtccgg ttacggcgag
    10441 gcgcagatgt ctgtcgacac cccgattcac cagcgcatta agttcatttt taagtcgctt
    10501 cattgctgtc ctgtgctgct ctgaagacca gaaaaaaatc attcgtaagg atatgaatgt
    10561 gggggccagg ccattgatta tcaactgccc gtgttgtcat cgacgggcga actccataag
    10621 atgtgcagca ttcacgttcg gacggagttc ctgacctatg tcccctacta tttatgatat
    10681 tgccagggtt gcaggcgtat caaaatccac cgtatcacgc gtgctgaata agcaaactaa
    10741 tatctccccg gaagcgcgcg aaaaagtgat acgggccatt gaagaattac agtatcagcc
    10801 aaacaagctg gcccgcgcgc tgacctcttc gggttttgat gccattatgg tgatttctac
    10861 ccgttcgacc aaaactacgg cgggtaatcc gtttttctct gaagttttgc atgccatcac
    10921 cgccaaagct gaagaggaag gtttcgacgt gatcttgcag acgtcgcaca atccggcaga
    10981 agacttacaa aaatgtgaaa gcaaaattaa gcagaaaatg attaaaggca ttattatgct
    11041 aagttcgcca gcggatgagt cattttttgc ccaacttgat aaatacgata ttcctgtcgt
    11101 ggtgattggc aaagttgaag gtcaatatgc ccatgtttat tctgtcgata ccgataattt
    11161 tggcgacagc attgcgttga ccgatgcatt aattgaaagt gggcatcaaa atattgcctg
    11221 cctgcatgct ccgcttgatg tccatgtttc agtggatcgg gtaaatggtt ataagcagag
    11281 cctggctgcg cataatattg cagtgcgtga tgaatggatt gttgacggcg gttataccca
    11341 tgaaacagca ctgcaagccg cgcgggaatt attaagccag tcaccgttgc ctgaggcagt
    11401 gtttgctact gacagcctga aattaatgag tatttatcgt gcggcagcag agaaaaatat
    11461 tgctattccg cagcagttag cggtggtggg ttatagtaat gaaacgctgt cgtttatttt
    11521 aacgcctgcg ccaggcggta ttgatatccc aacacaggag ttaggtcaac aaagctgtga
    11581 gttattattc cgcttaattt ctggaaaacc gtcaccacag aatattaccg ttgccacgca
    11641 tatgacgctg aaataaatac cagctacctt gcctgaatgc cggatgcgat gctagcgcat
    11701 cttatccggc ctacatttat agttcggata aagtactcgt gccgcatccg gcaatttagc
    11761 ccattatcag aacgagtaat ttacgccgac acctgcataa tggaatttat cgctgtcgcc
    11821 ttcgtcgtga tcctgccact caaacgcgta ttccagcgaa acggaaagcc cgttctggaa
    11881 atcataaccg taaaataaac ctacacggtt aaaatcatgg ccttcacgtt caatatcatc
    11941 ctgccagtcc cagttactcc agcgatccag cccaatgcgc gtatacggcg tcaccgagtg
    12001 gttgccgagc gtcagcggca aataggcgcg aatttcttgc gtagaaaact caccgttatt
    12061 gcggctgtcg tccatattaa agccgcgctc gagataatag ttcactcgca aggcaaccgt
    12121 ttcgttgaag gtatattgca ggcctgtttc cgtttcgact cgggtatcag cgtaaccggt
    12181 cgtgttcaga tcgttggcaa atttatacat cgacaaccaa ccgttgaaac gtaaatcgtc
    12241 agtcagtttc acatcccagt ctggcgcgat tttccagcgc tgcatattcg ccgtgtcttt
    12301 acccggttca tcaacatagt gataaccata attacggaaa ccgccggtca ggccgaaact
    12361 gaaatcatcg ttttcgagga actgataatg cacctccagc tccgggcgat caaaccacgt
    12421 tccacgttta cccgcgctat aatctaccgg cccttcctga taataggcca gagcaattct
    12481 ccacggcccg ttggcggcat taaaatagac tgaaggctcc gccagcccat ccatatcttc
    12541 gccataaccc tcgacgtttt ctatttcgta catcgcgccg atattaaagt gccagtcgtt
    12601 cctttcctcg gcctgtgcgc aggccattcc cgcacacatc accagtgcgg tacagggtaa
    12661 taactttttc attattgttc cttgtattat tctgggggaa tgccttcccc ctgtatttag
    12721 cgaattgcta tttccgtttc tgcatcaaag aaatgacatt tcgtcatatc aaaatgaata
    12781 gtgatatttt ctcctgcatg ataatcattt aacgcaccag cacggaccac taactcgtgc
    12841 cccccaaccg tggtgtagag cataaattcc gcaccggtta attctgccac gctaattttg
    12901 gcggaaatgt tattttcctc ttgcgcgtcc ggatgaatat cttccggacg tattcccatc
    12961 acgatgggct tatgcaaact tccctgtgtt ttcagaaccg ctaatttctc ttcgggaatg
    13021 gttaatttaa gcgtttccgt aacgaattta tcgccatcga tcgtgccacg aataaaattc
    13081 atcgccggtg atccaataaa tccggcaaca aacatattcg ctggttggtt ataaaccgtt
    13141 ttcggcgcac caacttgctg aacaatccca tccttcataa tcacaatccg cgtcgccatg
    13201 gtcatcgctt cggtctgatc gtgggtcacg tagatcatgg tggtattcag cttctggtgc
    13261 agcttgctga tctctgcgcg catttgcacg cgcagcttgg catcaaggtt agagagcggt
    13321 tcatccatta aaaacacgcc cgcttcgcgc acaatcgccc gcccgagcgc cacacgctgg
    13381 cgttgcccgc cggaaagcgc gcccggcttg cgtttcaggt actcacgcag gccgagaatt
    13441 tgcgccgccc agttcacccg ctcatcaatc acctctttgg cgattttttg catcttcagg
    13501 ccaaacgcca tgttgtcgta aaccgtcata tgcggataca acgcgtagtt ctggaacacc
    13561 atcgctatat tgcgtgcttt ggctggaacg tcattcattc gtttgccgtc gatcaacaga
    13621 tcgccgccgc tgatctcctc aagcccggca atcatgcgca gggtggtcga cttaccgcag
    13681 cccgacgggc cgacaaacac gatgaactct ttatcggcaa tttccaggtt gaagtccttc
    13741 accacatgca cctggttatc gtagattttt tgaatatgtt gtaacgaaag ctgagccatg
    13801 ttgattcctt tgctatacgt tttgccagaa ggccgataac cgcgaccagg tcagagagtc
    13861 cgttgaaggc aacagtaatt gcgccccggt taagcccgcg ccgatcccca ccgagcgcat
    13921 accgctggcg ttaatggcgt caatgcccgc ctgcgcatct tcaatgccga tacatgcctg
    13981 cggcggcacg cccagcccgg cacaggcggc gagaaagatt tccgggtccg gtttcgagtt
    14041 tttaagttgg gaagcatccg cgcagaaggt gaaaaactcg cgcagctcca gcgccgctaa
    14101 aatcgtcggc gcattcaggg agacagaagc cagcccaacc gggatctgct gtgcacggag
    14161 atctgccagc aaagagcgaa tgccgggtag aacagcgctg accgtcaact cgcgcagtga
    14221 gtggacatat agcagatttt tgcgatacgc cagttgcgcc ctctcctgcg agttaaagtc
    14281 gccctctttg cccccgtgtt gcagaatgcg ccgcagagac tcatcgcggc tgatcccttt
    14341 tagggattcg ttaaactgcg catcaatgct gatgccaatt tcagcggcaa tctgctgcca
    14401 cgcctggaaa tgcagatgcg cggtatcggt gattacacca tccagatcga aaattacccc
    14461 ttgcagtttc attcatcctc ctgatgtgtg gtagcggtcc cattgaaggg caaaataaaa
    14521 tcacccaaac agaaaacaga ttcttcagcc acggatataa gctgaccgtt caaacgcagt
    14581 gaaacgggcg cagaagttcg aatcgcaata cgctgcgcgt caagagtgac ctgtaattcg
    14641 cagccctgcc agaacagagg gaaagacaac tgttgccact gctcaggtaa cgccggattg
    14701 agatgcaatt caccgtcacg cacgctcacc ccggcaaaac cctgaatcgc ccccagccag
    14761 atagcgccag ttgcggcagc atggatacca tcatcacaac tatgcggatc agcaccaaga
    14821 tcgatttcag tcccctcgcg ccagaactga taactttggg tcagcaggcc acagcgtgcg
    14881 gcaacaatgc cgtggattgc tttacttaat gacgagtcgt gaatagtgcg cggttcataa
    14941 aattgcagat tggcaagaca cgatgccgct gagaactgct ccggcagcat gtaattgagc
    15001 atcaccacat cagcttgttt gaggatttgc atctcgttca cttctgcgcg tgaataatcc
    15061 agcagaatgg tttgcttccc cgccgccgct ttgtatttcg ccagattaat cgccggctta
    15121 gccataaacg aatcatcctg cggcaaaacg ccgtcgggct gaatttctgg cagccgcagt
    15181 tctttaagga acatttcggc gcgatggata aacgcatcgt cgctacagcc aaactggcgg
    15241 gcaatactca gcgcctgctg gacgttgtaa tacgccatat agctggtgaa ggcgttatta
    15301 ttgacatgtt cggtatattc gtcaggccca ataacatcat gaatttccag acggtcgtta
    15361 accctcaccg cgcggctaat ccagaacttt gcagtttcca gaagtagcgc catgccttca
    15421 tgcgcaatga aactttcatc ccccgtggtc tgccagtatt gaataaccgc ccaggcgata
    15481 tcggccacca gatgatgttc cgcctgcgcc gaggccactt tttgccgcag cccggtgcga
    15541 atgttaatgg cggcaaattc cggcgtctct tcttcgccgc tgcgcgcgct ttcccacgga
    15601 aatagcgcgc cctgccagcc gttgcgtcgc gctttctcct gcgcgcctgg caagttgtgc
    15661 cagcgataac gcagtaaact tcgggcaacc gtcggatcgc taaacagatg aaacggtaac
    15721 aaaaatactt ctgtatccca gaaaacgtgg cctttgtagc cttcgccggt taagcctttt
    15781 gccgcaatgc tgctgcgctc gtcgtgggca ggcgtcatga tgcgcagatg ataaagcgca
    15841 taatctaacg cttgctgatc gtgcgcttcg ccgccattta ccgtgatacg acgtttctgc
    15901 caccattgac gccagttttc tgttgatact gcaagaagtt ggtcgtaact ctgctgcgcg
    15961 cacatttcaa gctggcgaag cgacgcgctg ccccactcgt ctaaaacggc ttgcctgtca
    16021 tcccgccagt cgatccacac cagtttttgc aacgtcactg tctcgcctgc atgaagctgc
    16081 gcactggtat gttgctgcaa acggcgctct ttggcggtat agcattgctg cacatcaccg
    16141 ctcaccatac agcaacagct gatggccaca tcactgcgtc catcctgggt ggtgtagatc
    16201 ccctgcatca gatgctgacc aaacacccgc acctgggttt cgtcgagatg ttggcgaccg
    16261 tggttggttt gcgtggcgtc gatacctgtt gaaatcagca ctgacgcgtc ggcgtccagt
    16321 ggcgtaatag tgatttccag cacaatgagc ggcagttggt ctgccgaaac aaagcgacgg
    16381 ctggcgatgg tgtaacctgt gccgttgctg gtacgccaga caacgctacg gcgtaattct
    16441 ccactggcaa agtcaagttc acgctgccag gcttcgcggg ataacgagaa aacctcacca
    16501 tttatggcaa tctccatccc cacgacatca ggcaggttca tcagttcgtt gatttcacct
    16561 tttcccgccc gatgatacag ccccgccaga tacatccctc gcgtctggcg cgtgtaatct
    16621 tcttcatggc tggcgcgaag cccaagatag ccgttcccct gcgccatcag cgatgcatac
    16681 ttgttcaggg tatgctggct aaaatggggt tctgataacg ttactggcct ggtcataatt
    16741 ccacacgcgt ccctgtttct gcggattgat ataacgccgc caccagttgc tggatgatgt
    16801 acccctgctc ggcgtcggct atcatcacgg gcttgccctg tacgtgattg ataaaggctt
    16861 ccatgctgcg caaatggcgg ttgtcgtctg ccatttcccg ttgcatcagc gtcattaatt
    16921 caccgttgtt atcggtgtag atatgtgctg gaaacagcgt cgcaccggct ttatcaccac
    16981 aaaagctgac gttcataatc gactgttcgc ggatgttgag tgcaaatgac gtttccagcc
    17041 acagaatgcc gccgttatga aattcaatgg tgccaaacag cgaatcttcg acgctataag
    17101 ttgccggatc ccactcgcca aattgaccac agctcttttg cgtgccgatc ttttgaaagc
    17161 tatgcgcatt cacgcttttc accgccggaa agcccagcac atacatcgca gcatccagca
    17221 tatgaatgcc gatgtcgatc agcgggccac caccctgcag ttctttattg gtaaagacgc
    17281 cccagccagg aacgccgcag cgacgcaggg cgcgggcggt ggtaacgtaa atctctccca
    17341 aaacgccgtt ggtcacctgg tcacgcagct gttgcgtatc gagcgcaaaa cgatggtgaa
    17401 agtcgtaggc cagcaccttg cccagtttgc gcgcggtatc gcacatttcc cgcgcctgtt
    17461 ctggcgtcat ggcgggcggt ttttcgcaca tcacatggca gcccgcttcc agtgccatca
    17521 gggtatgttc gtaatgaaaa cggttaggtg agcagacgct aaccacatca ggtttcaccg
    17581 ccagcagcat ggcctgcgga tcgtcccaca cggatgcatt cccgtatttt tctgccagcg
    17641 cctgcgcctg ggaaaggcgg ctgtcacaga cagccaccag ttccagatcg ttgcgggtgc
    17701 agtagtacga agcatgaacc ttatccgcca cctggcctgc gcctattatc gcgacccgca
    17761 gcggagagct tgtcattgca cttttcacaa agacctctta gcaggtacgc aaccaggcca
    17821 acgaatcacg gtacgcctgg gcaggatctt ccgcccggat acgcccttcg tacactacat
    17881 agccctgata gttatccgcg cgcagctgtt caaacagcgc gtggaaatcc agggtgccgc
    17941 tgcccggctg gtagcgatgg ttatccgcaa tatgcacatg accgagcagg tcgcggttgt
    18001 catgcagcgc ctgcgccagg ttatcttctt cgatattcat gtgatagaaa tcgccgataa
    18061 tctgtacatg tttaagatcg ttttcgacga tgtaacggcg ggcatcggcg agggtgttga
    18121 tcatatgatc ctgatagcgg tttaacggtt cgagatacac cacggttccg gtacgcgcgg
    18181 cgacctgttc cagtacgcgc agggaatcac tcaccatttt gcggtcgccg tccaggctac
    18241 gcggcgaggt catcggcggt aagcggaagg taaacatgcc ccacgctgcc ggaacgacga
    18301 tacctttacc ccccacttct gccagcgctt cgagaatgcg ttcgatctgc tttaagccat
    18361 taagacgacg ctcttcgata aagtcgccaa tccaaccgtc atagccaccg caggcggtgg
    18421 tcaccggtaa accggtttct ttgattgctg ctttgacttc ttcgagattg ttgaccagca
    18481 gtttgccgtc aatctcaaaa ccatcgaagc ccatctcttt gatataacga aatttctcca
    18541 gaatgttttc cggaaagaat gcctgattct gtgtgcctat tttcattttt tcattccttt
    18601 gaattaaaac gtaacgccca ttttgatgct ctgttccgga tgctgatcga catactgcat
    18661 gtagctttcc gggctattgg caaaggtcac taccgggtcg attaaatctt cgcaattgag
    18721 ataaccgttc atcagcagtt cccagcaggt ttcttcaata cgcttacggc tccagcgcgg
    18781 ataatccggg ttcggttcgc tgcacgcgcg ggagaaaaca attttggcgt tattgaaatg
    18841 cgcttcgcgt ccgaggttaa aaccttcagc aaatggtttg gcaaacgcga cataagagat
    18901 ggtgccgcca taagcaaggc cacgcagcgc cgattgcagc gcgtcggcgt aaccgctggt
    18961 ttcgataatc acatcggcac cctgcttgcc ggtcagcgtt ttgatctctt ttcctacgtc
    19021 agtgccaatg gggttaaggc agaaatcagc accgtggcga cgggcaatat cacagcgata
    19081 ggcgatagga tcgacgccaa tcaccaccga cgcgccagcg cgtttcgcca gttggatggc
    19141 tatttgacca atcgcgccaa gccctaccac caccacaaaa tcacccacgc gcacgttggc
    19201 atcgcgcacg ccgctcatgg caaactgcgc cgggtcgtaa caaacggcgt ttttccacga
    19261 gctgccttgc ggcattttgc gcagcttgta gttattcacc gcgttaatga tgaccgtctc
    19321 ggagagcggg ccgtagccgc atacgctgtc gcccaccgcg tagtcggtaa cgtcgctgcc
    19381 gcactcgata atgtcgccaa ccaccatgtt gccaagctgg aatttgccaa actcaatgcc
    19441 gcgcggcgca tctgccggac gcggagtgaa catctgccat tcgccgttaa agtcttcatc
    19501 gataaacggg ctggcggcgc ggaagtcgac cacttccgtt ccgtgtttcg gtgcgccgaa
    19561 acgcacgcgg attttcactt cattggctaa aatcgcccga tcttcatact caaccagcgc
    19621 agcaacacgc ggtgctgtgg ctactaactt tttcatttaa aactccttgt aaagaaactg
    19681 gccgctttag cccttcacgc caccggcggt caaaccactc ttaataaaac gttccgaaag
    19741 cgcatacata atcaccaccg gcaatgcggt caccagtgag gccgccatca tccgtcccca
    19801 gatgtaatct ggcgtactga acagcgcgtt caggcccacc ggtaaggtga aattgctggc
    19861 gctggagagg aagatcgacg caaacagata gtcgttccac gccaccataa agcagtagac
    19921 gaacacggat atcagcccgg acatcgccaa cggcacggtg atgcggaaga taatttgcag
    19981 acggttgagg ccatccatca tcgccgcttc ttcgatctca tcggggatgg tgtcgaagta
    20041 gcttttcagc ataaacacgg cggtgggcag agtttgcgtc accatggtga tgatcagcgc
    20101 catctcagtg tcgtaaatgc ccagcgcggt gatgattttg aacagcggca ccaccagcaa
    20161 aatgccagag aacatataca ccgtgtaaaa gctggcgttg atggtcatcc gacctttaaa
    20221 gcgcaggcgg gaaagcgcgt aagccccaag aatgccgagg aataccgcca ccacggatga
    20281 aaccacagac accaccagac tgttacggaa gtagtcgaca aacgggaaaa tcatcgggtt
    20341 aaaaatgtcg acgtaatgct ccagcgtcca ctgctgcggc agcagcgtag gatgcagtga
    20401 gatcgcctct ttcgcgccct tgaacgaggt catcagcatc acaaaaaatg gaaacagcgt
    20461 gatgatgaga aacagcgcca gcccgcagta aaaaccgatg cgactgagtg tgcgtttatt
    20521 tgttgccatt gaggttcacc cgtttcctgg tcagcagaat gacagcgaaa ataatgatga
    20581 agagcaccac cgaaatcgcc gccgctttcc ctaagtcatt aaaggcaaag gcggttttgt
    20641 agagatagac gccgagaata tcgaccttgg tggtcagcag ataaacatca gcaaacatgt
    20701 agaacatcca gatagtgcgc agggtcacca ccgtcgccag gaccggcata attgcgggca
    20761 gcgtgacgat gcgaaaccgc tgccaggcat tagcgccatc catctctgca gcttcgtaca
    20821 gcgatttgtc gatggtctgc aaaatcgcca gaaacgagat aaaggcatac gggaagtagc
    20881 gccagatggc gaacagcacc accagcacaa agctactgcc cggattatcg aaccacagcg
    20941 gtgcctgctc atagagatgc agaagatcga cgccgaggta gttcacaatg ccgtagccgt
    21001 tgttgaacat gtatttccag gcaaacacca gcgaaatgga cggcgttacg taggagagga
    21061 tcaccagcga acgcgcggtt ttgcgcagat ggaactcgcg gttgaaaaac atcgccactg
    21121 ccagcccgag cacggtgctg cccgccacca ccagcgcggt ataccagacc gtcatccaca
    21181 gcgagtgcca gaagccggga tcggagagga tacgcacata gttactcacc ccaacaaagg
    21241 ttgactcgat gttgggattg agcggcagac gtaaaaaact gatttcgata ttcgacacca
    21301 tcggccacgc caccagaccg cccagcagta ataagctggg cgcgagaagc aacagcgcaa
    21361 agggcatatc ggaacgacct gaaaaaagcc tgttcattgc ggattcctgt ccggggttta
    21421 gtgctgttcg atcagttcat ccagcttttt ctggctcgct tgcagcgtgc cagggagatc
    21481 ggctttaccc acggtgacgt tatgcaccat ggagctcacc actccagacc ccgtcacatc
    21541 acccatgcgg gtaaagtttt tatcccctac tgcaccaaag acctgaatat ttggcagttc
    21601 accgattagc tgattcggta gttcacccag cgccttaata acgtcgttgt ctttccaggt
    21661 ggcggtagtg acaaccgctt tattcaccgg cagcgcagca cctggcgaca tcatcaccca
    21721 gtcggcaatg ttgtctgcct gctccataaa ggtgacaaat ttttctgctg cttccgtctc
    21781 ttcggtcttt tgcccggcgg taatggtcag cgaggtcaac atgccgtaga ccgcagagtt
    21841 tttctcggtt ggcaccacga aaccgacgtt tttcgggtcg ccttctttaa tcacagccgg
    21901 aaggatatag gtggagtaaa tcgccatcgg cgcggtgccg ttcataaagg cgtctttcac
    21961 ttccatgatg tcgttagaac ccggcatagt gttggcagca aggtcgcggt aataggtcag
    22021 tgcctgcatc atctctggtg tatcaagggt gattttgcct tcggcgttaa agacgttagc
    22081 ctggttggat aacgcaaact gggagaagga ttgttccgtc aacacgcttt ctgctgtagg
    22141 cagcgcaatg ccgtattttt tattcgccgg gtcattcagt ttctgtgcaa cgtccagcag
    22201 ctgttgccag tttttcggct cctcaagccc cgcttttgcc agcacatctt tgcgatacca
    22261 gataccgcca atccaggcgc tgacaggaac accggtccat gcgctaccat cttcggtacg
    22321 cacaatacgc agtacgccat cgtaaaacgc gccttcacca acgttgctga tgactgtggc
    22381 aaccgctttg cgatcgataa gctgctcttt gtccatcact ttggcgtagt catggctggt
    22441 ttcgatcact tccggcagcg aaccgctacg tgaaagagta atgactttag tgttataggc
    22501 atcttcttcc acgggcactt gcttaacggt gatgccaggg ttttcttttt caaaacgggc
    22561 aatcaattta ctgataacgg cctggcgctc ctgctccacc gaagaatgca taaattcgat
    22621 ggatacattc gttttatttt cttctttaca gcctgaaatc agggcgcagg aaaccagtgc
    22681 tgataacagc acgatttttg atttaatcat gagatgttcc tttttattta atccacataa
    22741 cctgccaggg gcgcagcgtt atttcattac ccaatatatc aacttcacta atcagatccc
    22801 gaccatgcag attagtaata tttacatgct gaatattttt actgacatta aacaatccag
    22861 tcagacaatt accatcagcg ttacttcttt gaatacgcat tacggatgaa ttaatcgtat
    22921 caatggtaaa attattatcc ggatgaaact cgttatggct gcgacgaagt gtaattaaac
    22981 gcgacaactc atgatatacc gcatgcctta atgtagcttc atcgttcagt tctcgggtta
    23041 tctctttact gtgatatttt ttacggttaa tcgcacggtt atatccgagt ttttcgacac
    23101 ctgcgtaatc attacgcgag ccaagaatac tttgaatata tatcgctggc acaccgggga
    23161 aacttaacaa aatcgcatgg gcgagaataa acctggcgca acgttcttca tcgctactct
    23221 cacggcggct taacgcatcc atataggtca catttatttc atacggactg cgtgtaccgt
    23281 cgggattatt tttccagttt actaatgcac cttcctgctg taacgcctcg accagctcta
    23341 atatttcgct ttcaggcaac aagccccgta gcgggtttag cccgatgcca tcgtgagagg
    23401 cgaggaagtt aaaccaggtg gtgttgctgg aaggtagtgt caggttttgc gcccacgcac
    23461 aaagcgcctc aacgttctgt ttttgcaccg catgcagcac cagtggcggc agcgagaact
    23521 ggtacaccat atgtgcttcg tcatcgcctg cgccaaagta agcaatgttg tctttatgcg
    23581 gaacattggt ctcggtaatg atcactgtac ctggcgcaac gttatcaata atcgaccgta
    23641 acagtttgat aatcagatgt gttttttcca gatggatgca gcttgttccc ggctctttcc
    23701 acataaagcc aacggcatcc aggcggacat actcagcacc tttcgcaagg taacagagta
    23761 aaacatccac catcgccagc aacacttcag ggctacggta attcaggtca atttgatcgt
    23821 cactaaaggt ggtccataaa tggcgcgttg aatgatcgcg catctggaat ggcgttaata
    23881 acggtaacgc acgcgggcga gtgacggcgc tgagatcggt ttgcgggtca acggcaataa
    23941 aaaaatcttc aaaacctgga tgctgttgta aatagttttt aaaccattca ctttttgccg
    24001 acatatggtt gcagacaaaa tcaaacatta aatgactaca ttcaccgagt tgctgaatat
    24061 cctgccactc ccccgcttca ctggcgacct gttgataatc aattaccgaa aagccatcat
    24121 cagatgacca tggataaaac ggcaacaaat gaacatgtga aaaaatgctt tgcagccatt
    24181 gatggtaaaa ctgattaaat gtaggtaatg gttttaaatc attgctgtga aattgatcgg
    24241 cataggtaat taacacgaca tcactttcat cccagtgttt tttacgtcgc tgtgtaatta
    24301 atcgtttcgc actctcaaga cgcgttacaa gtttctgtaa atgagttgcg gtaaatgttc
    24361 caccgtagat ttcgtccagg taatccgtaa ttttctgttt catgttgcct ttccgtggta
    24421 gcggtcccat gaaacgagag actactccgc agaaaataaa ctgtcaattg agcacaaggg
    24481 ttttagtcac gcaggaaatc attttttacc agggaaaaag cgtatttgtg agctgacgca
    24541 aataatctat ggggatgtaa ataaggtgtg acagaaaaaa aacggcgcat aagcgccgct
    24601 catggtgaat tcttttaacc tttgaccttc ggcattgcga tgtctttcag gccaccggcg
    24661 ttctcaacgt gggtatatcc catctcgcta aggatctctt ttgcttgccc tgactggcgt
    24721 ccggcattgc aatacacttt cacggtgtcg tttttatccg gaacggcggt ggcaatgcgc
    24781 tctttcactt ccttcagggg aatattgatg gccccctgaa cgtgctcttg ctgatactgc
    24841 tctggaacac gaacatcgat ccagtgttca gcggcgaaaa cgggcagtga aaacaccagt
    24901 gccagagcaa gtaagccttt tttaaacatg taagcgtcct tctatggatg aaaattgcca
    24961 tccattatac gcctgagatt taacaatttt tgcgaaactc ccctcacctt ttataacgct
    25021 gtgccagttt attagcagcc cgacttaaca gcggttccag cgccacagcc agcaacattt
    25081 tcagcggtcg gcgagcaact gattttatcg cccagcccgc cacacccgcc gggccatagc
    25141 gcagtgcggt aagaagtacc agcttgcctg ctaatttgaa accaagcttt accttttgcc
    25201 cggcctgttg ccagcgagta ttcataactt tcctcacagt tgacggaaac ggctacgtaa
    25261 cgtgaaagta tcggaagtga cataacgttc catctcgcgt aaacgcgttt cacttgccgc
    25321 cagttcacgg tcgacttcat ccagcaattc gctgctggaa ggtagctgct caccaaaggc
    25381 catgttgtcc ggcattggat caagcgcaaa tgacaaaatg atgtaagcaa ccagggtaaa
    25441 cagcgccaga ccgaagaaaa tcgacagcac caccaggata cgcaccagtt ttaccggtac
    25501 atcaaaatag ttggcaatcc cggcgcagac gccgcggacc atgccctgct gtggaatacg
    25561 ccataatttt ttattgagat taatgcccgc cattagcgat ccctccagtt cggatgttct
    25621 gcatcaagaa tagattccag cgcctgaata cgttcgcgca tccgttttgc ttcatcagcc
    25681 agttgcgcta atcgctgctg ctcactttgc gacaattcac tgcgaccaga acgattgctg
    25741 taatgcagcc ataaccagat cggtaaaaca aacagcacaa aaatggttaa cggaatagcc
    25801 agaaatagcg cgctcataag tactccttag acgatgagcg gcggcgcgtt cggacgccgc
    25861 ctggatgtta ttattgattg tcttgcttca ttttggcttt taattgtgcc agttgttcgc
    25921 tgattgcatc atcggctttc agttcggcaa actgatcgtc cagcgatttt tgtttaccga
    25981 agctgtggct ttctgcttcc gcttccatct ggtcaatacg acgttcgaaa gattcgaaac
    26041 gagccattgc ttcatccagt ttgccactgt ccagctgacg acgcacatcg cgcgacgagt
    26101 ttgccgcctg atgacgtaac atcaatgcct gctggcgagc gcgtgtttcg ctcaatttgt
    26161 tttccagctc accgatctct ttcttcatgc gtgccagcgt gtcgtccacc agcgtcactt
    26221 catgttccag gctcttaatc agatcggtca gtttctgttt ttcaattaac gctgcacgtg
    26281 ccagatcctc tctctctttc agcagcgcca gttcggcttt ttcctgccat tcaacttcac
    26341 gcgccgacgc ttgttcaata cggcgagtca gctgtttctt ttctgccaac gcgcgcgccg
    26401 aagtagaacg tacttcaacc agtgtatctt ccatctcctg gatcatcaga cgaaccagtt
    26461 tctgtggatc ttccgctttc tctaacagag cgttgatgtt ggcattcacg atgtcggcaa
    26521 agcgagaaaa aatacccata atgttgtcct cttgatttct gcgttcagga ttgtcctgct
    26581 gaactgttaa tacaatttgc gtgccaattt tttatctttt tgatttataa agatctgatt
    26641 gaagaatcaa cagcaacatg ccaggatgag ttagcgaatt acactaacaa gtggcgaatt
    26701 tcatcatggc agaatacaaa gataatttac ttggtgaggc gaacagcttt ctcgaagtgc
    26761 tggaacaggt ttcgcatctc gcaccgctgg acaaaccggt gctcatcatc ggcgaacgcg
    26821 gcaccggtaa agagctgatt gccagccgcc tgcattatct ctcctcccgt tggcaagggc
    26881 cgtttatttc ccttaactgc gcggcgttaa atgaaaatct gctggattcc gaactgtttg
    26941 gtcacgaagc gggggcgttt accggtgcgc aaaaacgtca tcctgggaga tttgaacgtg
    27001 ccgacggcgg tacgctattt cttgatgaac tcgctacggc accgatgatg gtgcaggaga
    27061 aattattgcg cgtgattgag tacggtgaac tggagcgcgt tggcggcagc cagccattgc
    27121 aggtgaatgt gcggttggta tgcgcgacga atgccgatct cccggcgatg gtcaatgaag
    27181 gcacttttcg cgctgacctg ctcgaccgac tggcttttga tgttgtacaa ctgccaccac
    27241 tgcgcgagcg cgaaagcgac ataatgttga tggcagaaca ctttgccatc cagatgtgtc
    27301 gggaaatcaa gctgcctctg ttcccggggt ttacggagcg cgccagagaa acattgctga
    27361 attatcgttg gccgggaaat attcgtgaat tgaaaaacgt ggtggaacgt tcagtgtatc
    27421 gccacggcac cagcgattat ccgcttgatg acatcattat tgatcccttt aaacggcgtc
    27481 cgcctgaaga cgctatcgcc gtttcagaaa ccacctcgct tccaacactg ccgctggatt
    27541 tacgtgagtt tcagatgcag caggaaaaag agttgctgca actcagtttg caacaaggga
    27601 aatataacca gaagcgcgcg gctgaattac tggggttaac ttatcatcag ttccgcgcgt
    27661 tgttgaaaaa gcaccagatt tagcgaagtt gagcaataca acttgcagga tgcggcgtga
    27721 atgctttatt cggcatacat aatcaatacg tcgtttgcag gcctgataag cgcagcgcat
    27781 cagacattat tgcgttgggc tattaatcgc tcagcgcatc ctgcaaaatt ttcattgccg
    27841 catcgaattg cgcatccggt atggtcagcg gatacaggaa gcgaatcacg ttgccgtatg
    27901 cgccacaggt cagcaggagc agcccctgcg ccagcgcgcg ttgctggatt ttctgtgcaa
    27961 tcgccgctga cggctcgccc gtttgcggat cgttaaactc tgccgcaatc atcgacccca
    28021 ggccgcgtac cgcagcaatg gccggaacgc tttctttggc atcaatcaac gtgtttttga
    28081 gacgctggcc cagttgattc gcgcgttcgc agagtgattc tttgtcgata atattgagca
    28141 ccgcgtgcgc ggcagccacc gccagcgggt tcccggcgta ggtgccaccc aacccgcccg
    28201 gcgcgggcgc gtccataata ttcgcgttac cgaccacgcc cgaaagcggc atcccgcccg
    28261 cgaggctttt cgccatcgtc attaagtccg gcttatcggc gtaatgatcc atggcaaaca
    28321 gtttaccggt acgcgcaaag ccgctttgca cttcatcggc aatcatcaca ataccgtgct
    28381 cgtcgcacag gcggcgaatg gcggcaacca gctcttttgg cgcaacgttg aaaccgccct
    28441 cgccctgcac cggttcgaaa ataatggccg cgacctgctt cgcttcgatg tctgatttaa
    28501 acaagcgttc gatggcgtcg agggagtcct gtgttgaaat gccgtgtaaa tctgacggat
    28561 aaggtacgtg atacaccgaa ccagggaacg ggccgaagcc gattttgtac ggcgcaactt
    28621 ttccggtcag cgccatggtc atatatgtac gaccgtggaa gccaccgcta aacgcaatca
    28681 cgccaggtcg tccggtatgg gcgcgagcaa ttttcaccgc gttttccacc gcttccgcac
    28741 cggtagtgaa gaatgcggtt ttagcctgcc cgctcactgg ggcaagggcg ttaattttct
    28801 ccgccagcgt gacgtagctt tcgtacggca caatctgata cgccgtgtgg gtaaactgtt
    28861 gcaattgctg ctccaccgcc gcgaccagat cagggtggcg atgcccggta ttcagcaccg
    28921 caatgcctgc ggcgaaatcg atgtattcgt tgccctctac gtccttcagc gtagcgtttt
    28981 ctgccgactg ggcgaagaag ttacacatca cgccaacccc gcgtggggta gcagaaagac
    29041 gacgctgatg gaattcattg ttgctcatgt ttcacacctt ttgactcttc actgttaatc
    29101 ggaaattaaa cgcccagacg atcgcgcagg ctgtaatatg ccgcgcccat cgcggtaaat
    29161 ggcacgcgca gcgtgcgccc gccggggaac gggtaatgcg gcagattggc gaaggcatcg
    29221 aaacgttcgg cgtcgccgcg cagcagttcg gcaatcagac gcccggctag atgagtacag
    29281 gtcacgccgt ggccgctgta gccctgcatg taatagatgt tggtatcaag gcgaccgaac
    29341 tgcggcatgc gcgacaaggt cagcaggaag ttgcccgtcc agcggtaatc aattttcacg
    29401 cccttcagct gcgggaaggt tttcagcagt ttcggcacca caaggcgctc aacgtcatcc
    29461 gggtcgcgcg cgccgtagac cacgccgccg ccgtacagca ggcggttgtc ggcggtaaga
    29521 cggtagtaat caagcagata gttacagtct tccacacagt agtttttcgg gatcagcgaa
    29581 cgggctaaat cttccgacag ccgttcggtg gtgatcacct gcgtgccgca cggcatgctg
    29641 cgtttcgcca gttccggctc tactttatcg cccagatacg cattcccggc gacaatcaca
    29701 tacttcgccg tcacctgacc tttggcagtt cgcacaacgg ctggcgtggt gtgctggatc
    29761 tgcgtcacgg cagaaagttc atacacgcgc ccgccgttga ggcggatggc gtccgcttcg
    29821 ccaatggcga ggtttagcgg atgaatatgc ccaccgctgt gatccagcag cgcaccggta
    29881 tagcgatcgc tggctacttc acggcgaatg gcgttggcgt ccagcaattc cagctgttta
    29941 ttgccgtagc gttcccagtt ctctttctgc tcttcaagtg tggcgagctg tttatcattc
    30001 atcgccacaa acaggccgcc ggggcggtag tcgcagtcaa tctgataacg tttgatacgt
    30061 tcgcggatga tctcaccacc ttcgaacatc atgctgccga gcatacgggc ggtgtccatg
    30121 ccgtagcttt tttcgatcac gtcgatgtcg cggctgtagg agttcacaag ctgcccgcca
    30181 ttgcgcccgc ttgcgccaaa gccgatgcgt gaggcttcga gaaccactac gtcaaagccc
    30241 gcttccgcca gatgcagcgc ggaggagagc ccggtatagc cgccgccaac cacgcaaacg
    30301 tcgcaggtga ttgactcatt cagcgtgtcg aatggcgcgt atttattcgc actggcggcg
    30361 taataactgc tggtatgttc ggtcatttca ggcctccagg cttatccaga tggttttcag
    30421 ttcggtgaat ttttcaaggg catgcaggga tttgtcgcga ccgttgccgc tctgcttata
    30481 gccgccaaac ggcacggtca tatcgccgtc gttgtagtta ttgacgaaga cggaaccagc
    30541 cttcaggcgg cggctcatgc ggtgcgcgcg ggagaggtcg cgcgtccata ccgccgcgcc
    30601 aaggccgtac tggctgtcgt tggcaagctg tagcgcctgg tcttctgatg tgaaacgcgt
    30661 gaccaccagc accggaccga aaatctcttc gcggcttaag gacgcattcg ggtctacatc
    30721 cacaaagatg gtcgggccga tggcggcagc cagctcagcg ttacgaccat ccagcagcag
    30781 ttgccctttg ctttcgcctt cccgaataaa gctatggacc gagtcggcgt gggcgcagtc
    30841 gattaaggtg cccatggtgg ttgcgggatc aagtggatgg cccggctgcc agttttgcgc
    30901 ctgctgtttt aacagggcta agaattcatc ggcgatgctc tcttccagca acaaacgcgt
    30961 tccggcgatg cacacctggc cctggttgta gaagatgcct gcggcggtgg cgcttgccgc
    31021 tttttgcaga tccggacaat cggcgaaaac gaggttggcg cttttgccgc ccgcttccag
    31081 ccagacgcgt ttcatgttgc tgtcgcccgc atctttcagc agctgtttcc cggtacgggt
    31141 tgaaccggta aaggcaatgg cgtcgatatc gttatgacgc gacagcgcct gccccgcttc
    31201 atgaccaaaa cctgtcacca cgttcaaaac accatccggc aagcctgctt ctttcgccag
    31261 ccccgcgaga cgaatcgcac tgagcggtga tttttcagac ggtttcagga caacgctgtt
    31321 ccctgctgcc agcgccgggc caagcttcca gcaggtcagc agcagcggaa agttccacgg
    31381 tacgatggcg gcaatcacac cgaccggttc acgcacgatc atcgccagct catggctact
    31441 ggtggtcgcc acttcgccat acactttgtc gatcgcttcg gcgtaccagc gaatggcgcg
    31501 cgccgcgccg ggaatatcat cacgcagact gtgacgaatc ggtttgccgg tgtcgagagt
    31561 ttccagcagt gccagctctt cggcgtgggc ttccattaaa tcggcgagtt tattcagtac
    31621 cgctttacgt ttagccggag aagagagtga ccagtcgccg cgttcaaata cgccgcgtgc
    31681 tgcgctcacc gcacggtcga tatcgacgct cttgccgcgg gcaattttcg ccagcggtgc
    31741 ctgggtgacc ggatcaacgg tttcaaaggt ttcattttcc gccgcagcag tatattcacc
    31801 gttaataaat aagcggtttt caatggcgag acttaacgct ttatcctgcc agtaagccag
    31861 atgatgaaaa ttcattatga ctcctgtttc acgtctatca gatatatgca gtttgtgaac
    31921 gtcagggcgc agagatcccc gggccgtatg cccgtttatt tctggcgaca ttctttatat
    31981 acacttcatc cttcaagccg cctctgcctt ggctgcgttc gctcccccga atcacttacc
    32041 tgagtaagct catcgggatt cgctcacttg ccgccttgat gcaacttgaa tgattttgtg
    32101 tatagattgc cgggcgcatt gcgcttaccc ggcttacaga acaaaaagat taaaacgtgg
    32161 tgggcgtatg ggcgctgata attcggcaaa tacctgccga cgtattactg aaactgtgcg
    32221 ggatgccggt attaatggca taactttgcc ccgcgacgag atgataatcc tgaccattaa
    32281 tcgtcagaac aatttcacct tccagtacag tgcctatttc ctcaccctga tgcttaattc
    32341 tttccccagt ggttgtgcca ggctggtaag tttcaaagat catcgccagc gtgcgattcg
    32401 ggttaccgtt atgaaccagc ttcattgaca caccctgact gcccatctca attaagtcgt
    32461 cctgattaat gacgacctgc ggctcatcag gtttttccgg ctcggaaaag aattccgaga
    32521 gtgacagacc atacaccttc agcagctttt gcagcgtact gatggcaggg ctgactttat
    32581 cttgttctat cgtactgata gcactgtgag tcagcccgga gagttcggcg gcacgacgtt
    32641 gtgaaagccc ctgctgctgg cggatttccg acaagcgttt tcctggcgcc agtccctcat
    32701 cactcatatt tgcatttcct taaactgtag tagtcagagt cgctgttttt cagcgatatg
    32761 gtgctgacaa gcggtgataa agccctcgaa caatatacgc gaaagcgcgt actcgctact
    32821 gttccattcc gggtgccact gtacgcccag cgcaaaagga tgattgatga cgctaaccgc
    32881 ctcaactaag ccatccggcg agcgagcttc aacacgcaac cgtgggctaa cgaccttcgc
    32941 cccttgtcca tgcagagagt ttacccaaaa gttgctacat tcaggtaaca acgcagacag
    33001 taatcccccc tcttcaacct gaacttcatg cgacggtgca tattgctgtt ccaccggcag
    33061 ttcgggatct tcccgatgtt ccagcaattc aggctgttcg cacagcttgc gatgcaacga
    33121 accaccggtt gccaccacca gttcctgtaa accccggcag atggcgaaaa tggggatgcg
    33181 cctttcgagt gcggcattaa ttaacgccat gctcagaaga tcacgcccgg gatcggcgtc
    33241 aggctcatcg ccgttttcac catataggtg cggctgcaca ttgctgggac taccaggaag
    33301 ataaatgcca tcgagtttcg gcaaaagttg ttcaagtaac gacggttccg ccagcgcatg
    33361 tggtagcgca attggcaagc cgcctgcgtg gatgatggca ttcaggtact tttcttgcag
    33421 agtctgggtc gcatgaccct taagcctgtt cctgcacatt acgacaccga taaccggatt
    33481 gttcattata ttttccatgc tcaatctcac aaagtggact aaattatcgc cattactgcg
    33541 ctttaaatga cctttatgtt caatattttt tcaatctagc agtgggtttt catttttgca
    33601 aactcaattt aacatttgac aaacatttag tttgcataca gattcgaatg gtggtcatta
    33661 tattttacgc tttgataacg agcggaaaac aaaccaaagg cgaagaatca tggaaaccaa
    33721 tatcgttgaa gtagagaact ttgttcagca gtcagaagag aggcggggta gcgcctttac
    33781 gcaggaagtg aaacgctacc tggagcgcta cccgaatacg caatatgttg atgttttact
    33841 tactgatttg aatggttgct tccgtggaaa acgtatccct gtttcaagcc tgaagaagtt
    33901 ggagaaaggg tgttatttcc cggcctcggt gtttgcaatg gatattctgg gcaacgtagt
    33961 agaagaagcg ggtctgggtc aggaaatggg cgagccggat cgtacttgtg ttcctgttct
    34021 cggttcctta accccttctg ccgccgatcc agagtttatc ggtcagatgc tcctgaccat
    34081 ggtcgatgaa gatggcgctc cctttgacgt tgagccgcgg aacgttctta accgcctgtg
    34141 gcagcagctg cgccagcgcg gattgttccc ggtcgtagcg gtagagctgg agttctattt
    34201 actggatcgc cagcgcgacg ctgaagggta tctgcaaccg ccctgcgcgc ccggcaccga
    34261 tgaccgcaat acacaaagcc aggtttactc cgttgataac ctcaaccact tcgccgacgt
    34321 gctcaatgat attgatgaac tggcgcagtt acagttaatt cccgccgacg gtgcggtcgc
    34381 cgaagcctcg ccgggtcagt ttgaaatcaa cctttaccat accgataacg tgctggaagc
    34441 ctgcgatgat gcactggcgc taaaacgcct ggtgcgtctg atggcagaaa agcataagat
    34501 gcacgccact tttatggcga agccgtatga agagcacgcg ggcagcggga tgcatatcca
    34561 tatcagtatg caaaataatc gtggcgagaa tgtgctttct gacacagaag gcgaagattc
    34621 gccgctgctg aaaaagatgc tcgccgggat gattgacctg atgccgtcgt cgatggcgtt
    34681 gctggcacca aacgtgaact cgtatcgccg cttccagccg ggaatgtatg tgccgacgca
    34741 ggcgtcgtgg ggccataaca accgcaccgt cgccctgcgt attccgtgcg gcgaccgtca
    34801 taatcaccgc gtggaatatc gcgtggcggg tgccgatgcc aacccatatc tggtgatggc
    34861 agcgattttt gccggtattt tgcatggcct tgataacgag ctgccgttgc aggaagaagt
    34921 cgaaggcaac gggctggaac aggaaggctt acccttcccg attcgccaga gcgatgccct
    34981 gggtgagttt atcgagaatg atcacctgcg ccgctattta ggcgaacggt tttgccatgt
    35041 gtatcacgcc tgtaaaaatg atgagctgtt gcagtttgag cgcctcatca cagaaacaga
    35101 aattgagtgg atgttgaaaa acgcgtaatt ccccatgcgg ggttgtttca tcggcctgag
    35161 cggagccagg cctgacattt gctggtaatc cagacatttc ccggatgtcg cgttatggag
    35221 cgcaggcggc aacgggcttc cgttaacgat taagactgtg acgaggaaat gagatgatgc
    35281 cgatgattaa atccccgcat ggcgagggag gctgcgtatg cgcccctcct gctactgact
    35341 ggactccgcc ccctttgctt cccttgctaa acaggtttga tttcaggttg acacgaccgc
    35401 aaaccttatt acgcagggga ggcagcaatt atggctatta attcaccact gaatattgct
    35461 gcgcaacccg gcaaaacccg tctgcgaaaa tcactgaaat tgtggcaggt ggtaatgatg
    35521 ggtctggcct atctcacgcc gatgaccgta tttgatactt ttggtattgt ctccggcatt
    35581 agcgacggtc acgttccggc gtcctatttg ctggcgctgg cgggcgtgct gtttaccgct
    35641 atcagctacg gcaaactggt tcgccagttt ccggaggccg gttcggccta tacctacgcg
    35701 caaaagtcga ttaatccgca cgtcggattt atggtcggct ggtcatcact gctggattat
    35761 ctctttttgc caatgatcaa cgttttgttg gcgaaaatct atctctccgc cctcttcccg
    35821 gaagtgccgc cgtgggtgtg ggtggtaacc ttcgtcgcca ttttaaccgc cgcgaatctg
    35881 aagagcgtca acctggtcgc taacttcaat accctgtttg tactggtgca aatctccatc
    35941 atggtggtgt ttatcttcct ggtagttcag ggactgcata aaggagaagg cgttggcacc
    36001 gtctggtcac ttcagccgtt tatcagcgag aacgcgcacc tgatcccgat tattaccggt
    36061 gcgacgattg tctgtttctc gttcctcggt ttcgatgcgg tgaccacgct ttcggaagag
    36121 acgccggacg ccgcacgcgt gatcccgaaa gccatcttcc tgacggcggt atacggcggc
    36181 gttatcttta tcgcggcgtc gttctttatg cagctgttct ttcccgatat cagccgcttt
    36241 aaagacccgg acgccgcact gcctgaaatt gcgctgtacg tcggcggcaa gctctttcag
    36301 tcgattttcc tctgcaccac gtttgtgaac acattagcgt ctggcctggc ttcacatgcc
    36361 agcgtgtcgc gtctgctgta tgtgatgggg cgtgacaatg tgtttccgga gcgcgtgttt
    36421 ggctatgtgc acccaaaatg gcggactccg gcactcaacg tcattatggt cgggattgtc
    36481 gcgctgtcgg cgctgttctt tgatttagtc accgcgacag cactgattaa cttcggtgct
    36541 ctggtggcgt ttaccttcgt gaatctgtcg gtgtttaatc atttctggcg gcgtaaagga
    36601 atgaataaaa gctggaagga tcacttccac tatttgctga tgccgctggt tggcgcgctg
    36661 acggtgggtg tgctgtgggt taacctagag tcaacgtcac tgacactcgg tctggtatgg
    36721 gcttcgttag gcggcgcata tttgtggtat ctgatccgcc gctatcgcaa agtgccgctg
    36781 tacgaaggtg acagaacgcc ggtgagcgaa acgtaatagc cggatgcgcg gcgcattatc
    36841 ctgcctgagg gtaacgcgcc gcgccattaa ctataaaaag atgtatgacc cggatcacga
    36901 gtgctatcat ccgcccttat gcttagcgca ttaaccccag gggcaaataa tgaaccacga
    36961 tatcccgctt aaatattttg atattgccga tgagtacgcg accgaatgcg cagagcctgt
    37021 cgcagaggct gaacgcacac cgctggccca ctacttccag ttactgctca cccgtttgat
    37081 gaacaatgaa gagatcagcg aagaagccca acatgaaatg gctgctgaag cggggattaa
    37141 tcccgtacgc attgatgaga tcgcagagtt tttgaatcaa tggggtaatg agtaattatt
    37201 taattgcaga cgatcggtca gtatcgttgt ccggataaag cgttcacgcc gcatccggct
    37261 aacaatgaca cgcactgcag cgtgctttaa tgggtttctc acgatcgtac cgctctgctc
    37321 ctcgctaagt tgttctgtga gttatagtta tagagaaatc acgggtacga atgttgaaca
    37381 gatggcgacg ccacctgttc aacgaccaac cacacggccc tcattacgac ccctgaacac
    37441 aaacggtaat ggcaaatgtc attaaatatt agtaagatag cctctttaca taaaatccct
    37501 taatattatg cgccaggtat tatcgtctct tttggtgatt gctggacttg tgagtggtca
    37561 ggcaatcgcc gcgcctgaat cccccccgca tgctgatatc cgcgacagcg gttttgtcta
    37621 ttgcgtcagc gggcaagtca acacctttaa cccatccaaa gcgagcagtg ggttaattgt
    37681 cgataccctt gccgcccagt tttatgatcg actgctggat gtcgatccct atacctatcg
    37741 cctgatgccg gaacttgccg aaagctggga agtactcgac aacggcgcga cctatcgctt
    37801 ccacctgcgt cgcgatgttc cgtttcaaaa aaccgactgg tttactccca ctcgtaaaat
    37861 gaatgccgac gatgtggtgt ttaccttcca gcgaattttt gaccgcaaca acccgtggca
    37921 taacgtcaac ggcagcaact tcccctactt cgacagcctg caatttgccg ataacgtgaa
    37981 aagcgtccgc aaactggata atcataccgt tgagttccga ctcgcccagc cggatgcttc
    38041 ttttttgtgg cacctcgcaa cccattatgc ttcggtcatg tcggcagaat atgcccggaa
    38101 gttagagaaa gaagatcgcc aggagcaact cgaccgtcaa ccggtcggca ccggtccgta
    38161 tcagttgtcg gaataccgcg ccgggcaatt tattcgccta caacgtcatg atgacttctg
    38221 gcgcggtaaa ccgttaatgc cgcaggtagt ggtggattta ggctccggcg gcaccggacg
    38281 tctgtcgaaa ctcctgaccg gggaatgcga cgttctggcc tggcctgctg ccagccagct
    38341 atccattttg cgtgacgacc cgcgcttgcg tttaacgctg cgtcctggga tgaacgtcgc
    38401 ctatctggca tttaacaccg ccaaaccgcc gctaaataat cccgctgtcc gccatgcgct
    38461 ggcactggcg attaataacc agcgcctgat gcaatccatc tattatggta cggctgaaac
    38521 ggcggcctct attttaccgc gcgcctcgtg ggcctatgac aacgaggcta aaattactga
    38581 atacaatccg gcgaaatcgc gcgaacagtt gaagtcgttg gggctggaaa atttaacgct
    38641 gaaactgtgg gtgcccacac gttcgcaggc gtggaacccc agtccactga aaactgccga
    38701 actgattcag gcggatatgg cgcaggttgg cgtaaaagtg gtgattgtgc cggtagaagg
    38761 tcgctttcag gaggcgcggt tgatggatat gagccatgat ctgacgttat ccggttgggc
    38821 gacggacagt aacgacccgg acagtttctt ccgtccgtta ctgagctgcg cggcaattca
    38881 ttcgcagacc aacctcgccc actggtgcga tccgaaattc gacagcgtat tgcgtaaggc
    38941 gctctcttcg cagcagctgg cggcgcgtat tgaagcctat gacgaagcgc agagtattct
    39001 ggcgcaggaa ttgcccattt tgccgctggc gtcgtcattg cgtttgcagg cctatcggta
    39061 cgatatcaaa ggtctggtac ttagcccgtt tggtaacgcc tcctttgctg gggtgtatcg
    39121 cgagaaacag gatgaggtga aaaaaccatg attatcttca ccttacgccg cattttgtta
    39181 ttgattgtca ccttgttcct gctgaccttc gttggcttca gcttgagcta tttcacccct
    39241 catgcgccgt tacaaggcgc gtcactgtgg aatgcctggg tgttctggtt taacggcctg
    39301 atccactggg attttggtgt ttccagcatt aacggtcaac cgattgccga acagttaaaa
    39361 gaggtcttcc cggcgacgat ggaactgtgc atcctcgcct tcggttttgc gctgattgtt
    39421 ggcatccccg tggggatgat tgccgggatt acgcgccata agtggcagga taacttgatc
    39481 aacgccatcg ccttactggg cttttcaatc ccggtgttct ggctggcgct tctgttgacg
    39541 ctgttttgtt cactcacgct aggttggttg cccgtttcag ggcgtttcga tctgctctac
    39601 gaagtgaaac cgattaccgg ttttgcgttg attgatgcct ggctttcgga ctcaccatgg
    39661 cgggatgaaa tgatcatgag cgcaatccgc catatgatat tgcccgtgat caccctgtcg
    39721 gttgcgccaa caactgaagt gatccgcctg atgcgtatca gcactatcga agtgtatgac
    39781 cagaactatg taaaagcggc ggcgacccgc ggtttgtcgc gctttactat tttgcgtcgc
    39841 cacgttctgc ataacgcgtt accgccggtt attcctcgcc tgggcttaca gttttccacc
    39901 atgctgacgc tggcgatgat caccgaaatg gtttttagct ggccgggttt agggcgctgg
    39961 ttaattaacg ccatccgcca gcaggattac gcagccattt ccgccggagt gatggtgtgt
    40021 ggctcactgg tcattattgt taacgtgatt tctgatattt tgggtgccat ggctaaccct
    40081 ctgaaacata aggaatggta tgccttacga tagcgtatac agcgaaaagc gcccgccggg
    40141 cacgctgcgt accgcctggc gcaaatttta tagtgacgcc tctgcgatgg tcggcctgta
    40201 cggctgcgcg gggctggctg tactgtgtat ttttggcggc tggtttgcgc cttacggcat
    40261 cgaccagcaa tttctcggtt atcaattatt gccgccgtca tggtcgcgct atggcgaagt
    40321 ttctttcttc ctggggactg acgacctcgg acgcgatgtt ttaagccgct tactgagcgg
    40381 agctgcgcca accgtgggtg gcgcatttgt ggtgacgctt gccgcgacga tctgtggcct
    40441 ggtactgggc accttcgccg gggcgacaca cggcctccgc tcagcggtgc ttaaccatat
    40501 tctggatacc ttactggcga tcccttcgct gctgttggca attatcgttg tagcgtttgc
    40561 cggaccgagt ttgtcccacg ctatgtttgc tgtctggctg gcattgctgc cgcgtatggt
    40621 acgttcgatt tacagcatgg tgcatgacga actggaaaaa gagtacgtta tcgccgcccg
    40681 tctggatggc gcatcaacgc tgaatattct ctggtttgct gtgatgccaa acatcaccgc
    40741 cgggttggtc accgagatca cccgcgcact gtcgatggca attctcgata tcgccgcgct
    40801 gggctttctc gatctcggcg cacaactccc ctcgcctgaa tggggagcga tgctcggtga
    40861 tgcgctggaa ctgatttatg tcgccccgtg gactgtcatg ctgccaggtg cggcaattat
    40921 gattagcgtg ttgttggtta acctccttgg cgatggcgtt cgtcgtgcga ttattgcggg
    40981 ggtggaataa tgccattact cgatattcgt aacctgacca ttgaatttaa aaccggtgat
    41041 gagtgggtta aagccgtcga ccgggtaagt atgaccttaa ccgaaggtga aatccgcggt
    41101 cttgttggtg aatccggttc cggcaagagt ttgattgcga aagcgatttg tggggtgaat
    41161 aaagataact ggcgtgttac tgctgaccgt atgcgttttg atgatatcga tttgctgcgt
    41221 ctctccgcac gcgaacggcg caaactggtt ggccataacg tgtcgatgat tttccaggaa
    41281 ccgcagtcgt gtcttgaccc ttcagaacgt gtgggtcgcc agttgatgca aaacatccca
    41341 gcctggacct ataaaggccg ttggtggcag cgttttggct ggcgcaaacg ccgtgccatt
    41401 gaactgctgc accgcgtggg gattaaagat cacaaagatg cgatgcgcag ttttccctat
    41461 gagttgaccg aaggtgaatg tcagaaagtg atgatagcca ttgcactggc gaatcaaccg
    41521 cgtctgctga ttgctgacga accgaccaac tcaatggagc caacaaccca ggcgcaaatc
    41581 tttcgcctgc tgacgcgtct caaccaaaac agcaatacca ctattttgct tatcagccat
    41641 gacttacaaa tgcttagcca atgggcggat aaaattaacg tgctttactg cggtcaaaca
    41701 gtggaaaccg cgccgagtaa ggagttggtg acgatgccac atcatcctta tacccaggcg
    41761 ctgatccgcg cgataccaga cttcggcagc gcgatgccgc ataaaagtcg cctcaatacg
    41821 ctgcccggcg ctatcccact gctggaacag ttaccgattg gttgtcgtct ggggccacgt
    41881 tgcccgtatg cacaacgaga atgcattgtg acgccacgtt tgacgggggc gaaaaatcat
    41941 ctctatgcct gtcatttccc gctgaacatg gagaaagagt gagatgatcg aaacgctgct
    42001 tgaagtgcgt aatttaagta aaacgttccg ctaccggacc ggctggtttc gtcgtcagac
    42061 cgtagaagcg gtaaaaccct tgagctttac gctacgtgaa ggccagacac tggcgattat
    42121 tggcgagaat ggttcgggta aatccacgct ggcaaaaatg ctggcgggaa tgatagagcc
    42181 taccagcggc gagttattga ttgacgatca tccactgcat tttggcgatt attccttccg
    42241 cagtcagcgt attcgcatga tttttcagga tccttcgacc tcgttgaatc cccgtcaacg
    42301 tatctcgcaa attctggatt ttccactgcg cctgaacacc gacctggaac cggaacagcg
    42361 tcgcaaacag attattgaaa cgatgcgtat ggttgggctg ctaccggatc acgtcagtta
    42421 ctatccgcat atgttggcac ccggacaaaa acagcgtctg ggtctggcgc gcgcgctgat
    42481 attgcgccca aaagtcatta ttgccgatga agccctcgct tcactggata tgtcgatgcg
    42541 ttcgcagttg attaatctga tgctggagtt acaggaaaaa cagggcattt cgtatattta
    42601 tgttacccag catatcggaa tgatgaagca cattagcgac caggtgctgg tgatgcatca
    42661 gggcgaggtt gtcgagcgag gcagcaccgc ggatgtgctg gcatcaccgc tgcatgaact
    42721 caccaaacgg ctgattgccg ggcactttgg cgaagcattg acagcggatg cgtggcgtaa
    42781 agatcgctaa ttttctgaat cctccttcca gattattcac ctcattctga caagagatta
    42841 aatcttgtca gaatgaggtg atggataaaa taaaatcaaa tgcgcgtgat ttaagaagca
    42901 atctcacttt gcaggaacga aagctctggc gatatcttcg cagccgacgt tttggtgatt
    42961 tcaaatttcg ccgtcaacat ccggtgggga gctacattct cgattttgct tgctgctcgg
    43021 cgcgtgtagt cgttgagctg gatggtgggc agcatgattt ggcagttgcc tatgattcca
    43081 ggcgcactag ctggcttgag tcgcagggct ggaccgtgct gcgtttctgg aataacgaga
    43141 ttgattgtaa tgaggagacg gtgctggaga atattctgca ggaactgaac cgccggtcac
    43201 cctctccctg aaagagcgag ggggccgacc gagccgaata gctgttgtgg tgaaaatatg
    43261 gtgacagttc tggagaatat tcggcaaggt ctgaaccgtc ccagccatcg ccctgaaagg
    43321 gttagggggc tgtataagcc tgtttgttgc tggggtgaca atatttgcac aatacggtcc
    43381 cctcgcccct ctggggagag gattagggtg tggggaaaag cgcccaccct gcccgcaccc
    43441 tgctccggtc ggacctggca gctatagcca ctcacagcca ggttgattat aataaccgtt
    43501 tatctgttcg tactgtttac taaaacgacg aatcgcctga ttttcaggca caacaagcat
    43561 caacaataag gattaaagct atgggttttc tttccggtaa gcgcattctg gtaaccggtg
    43621 ttgccagcaa actatccatc gcctacggta tcgctcaggc gatgcaccgc gaaggagctg
    43681 aactggcatt cacctaccag aacgacaaac tgaaaggccg cgtagaagaa tttgccgctc
    43741 aattgggttc tgacatcgtt ctgcagtgcg atgttgcaga agatgccagc atcgacacca
    43801 tgttcgctga actggggaaa gtttggccga aatttgacgg tttcgtacac tctattggtt
    43861 ttgcacctgg cgatcagctg gatggtgact atgttaacgc cgttacccgt gaaggcttca
    43921 aaattgccca cgacatcagc tcctacagct tcgttgcaat ggcaaaagct tgccgctcca
    43981 tgctgaatcc gggttctgcc ctgctgaccc tttcctacct tggcgctgag cgcgctatcc
    44041 cgaactacaa cgttatgggt ctggcaaaag cgtctctgga agcgaacgtg cgctatatgg
    44101 cgaacgcgat gggtccggaa ggtgtgcgtg ttaacgccat ctctgctggt ccgatccgta
    44161 ctctggcggc ctccggtatc aaagacttcc gcaaaatgct ggctcattgc gaagccgtta
    44221 ccccgattcg ccgtaccgtt actattgaag atgtgggtaa ctccgcggca ttcctgtgct
    44281 ccgatctctc tgccggtatc tccggtgaag tggtccacgt tgacggcggt ttcagcatcg
    44341 ctgcaatgaa cgaactcgaa ttgaaataat cattctgttg gtaaatattg ggcggcgttc
    44401 tgccgcccgt tatctctgtt atacctttct gatatttgtt atcgccgatc cgtctttctc
    44461 cccttcccgc cttgcgtcag gataacgatt tcctttacga ccaaggagcg cccatggaac
    44521 aacgccacat caccggcaaa agccactggt atcatgaaac gcaatccagt actgcggagt
    44581 atgacgttct gcctctggtc ccggaagccg caaaggtcag cgatcccttt ctgctcgacg
    44641 tgatccttga tgaagaaacg ctggctccct tcctttcatg gctggtccct gcgcgcgttc
    44701 ttgcagtgga attgttccct gaccagctta ccgtgacccg ttcacagacc ttcaccgctt
    44761 atgaacgctt gtctacggcc ctgacggttg ctcaggtttg cggcgtccag cggttatgta
    44821 actactattc ggcgcgactt acgccgctcc ccgggcctga ttcctccagg gaaagcaatc
    44881 ataggttggc acaaatcacg caatatgccc gccaactggc tagctcgcct tctattatcg
    44941 acaaccgatc gcgccagcat ctgaatgacg tcggtcttac tgccggggac tgtgtgatca
    45001 ttaaccaaat cattggtttt attggctttc aggcgcggac cattgcgaca tttcaggctt
    45061 atctcgggca cccggtacgc tggttacccg gtctggaaat acaaaactac gccgacgcgt
    45121 cactgtttgc tgatgaatca atacgctggc gaagcagcta tgaggtggaa aaactacctg
    45181 aagagtacac aaaaagttca acagcagaac tttgccaact ggccgaaaca ctctctctcc
    45241 accctatttc actttccctt ctcgaaaggt tgttaaacag tacacgggtt aatacacagc
    45301 cggataataa gcttgcggcg ttgttatgcg cgcggataaa tggcagtcct gcttgttttg
    45361 ccgcctgtat ggattcatca aatgaatata aaaaaatcag cccccttctg cgcaagggcg
    45421 aaaatgaaat taaccaatgg gctgaccgtc attctgttga gcacgctacc gttcaggcga
    45481 tacaatggct gacccgagca cccgatcgct ttagcgccgc ccagttcagc cctttactcg
    45541 aacacgaaaa atcatcaacg cagattatta atctgctggt atggagcggg ctgtgtggct
    45601 ggataaatcg cttaaaaatc gcgttgggtg agacatatta accttgccgc gtcagacaga
    45661 ttcgcgtaaa actgtcagcc gctctaatgg ccaccaaaat agacaattat gtttcaggac
    45721 aacccgctgc tagcgcagct taaacagcaa ctgcattccc agacgccacg cgctgaaggg
    45781 gtggtaaaag ccacagaaaa aggctttggc ttcctggaag tcgacgcgca aaaaagttat
    45841 ttcattccgc cgccgcagat gaaaaaagtc atgcatggcg accgaattat cgcggtgatc
    45901 cacagtgaaa aagaacgtga atccgcagag ccagaagaac tggttgaacc gttcctgact
    45961 cgtttcgtgg gtaaagttca gggcaaaaat gaccgtctgg ccatcgttcc tgatcatcca
    46021 ctcttaaaag acgccattcc ttgccgcgca gcccgtggcc tgaaccacga gtttaaagaa
    46081 ggcgactggg cggttgccga aatgcgccgt catccgctga aaggcgatcg ttctttctat
    46141 gctgaactga cacaatacat cacttttggt gacgaccact ttgtaccgtg gtgggttacc
    46201 cttgcgcgcc ataatctgga aaaagaagca ccagacggcg tcgctaccga aatgctcgat
    46261 gaaggtctgg ttcgtgaaga tctgaccgcg ctggattttg tcaccatcga cagtgccagc
    46321 acagaagata tggatgacgc ccttttcgct aaggcgttgc cggatgacaa acttcagctg
    46381 attgtggcga ttgccgatcc aaccgcgtgg attgctgaag gcagcaagct ggacaaagcc
    46441 gcgaaaattc gcgcattcac caactatctg cctggcttca acatccctat gctgcctcgc
    46501 gagctttctg acgatctctg ctcactgcgc gccaatgaag tccgcccggt actggcatgc
    46561 cgcatgacgc tctccgctga tggcaccatt gaagataata tcgaattctt tgccgccacc
    46621 atcgaatcca aagcaaagct ggtgtatgac caggtttctg actggctgga aaataccggt
    46681 gactggcagc ctgaaagtga agcaattgcc gaacaagtcc gtttgctagc gcaaatttgc
    46741 caacgccgcg gcgagtggcg tcataaccac gcactggtgt ttaaagatcg cccggattac
    46801 cgctttattc tcggtgaaaa aggtgaagtg ctggatatcg tcgccgagcc tcgtcgcatt
    46861 gccaaccgta tcgtcgaaga agcgatgatt gccgctaaca tttgtgcggc ccgcgtactg
    46921 cgcgataagc tcggttttgg catctataac gtgcatatgg gctttgatcc ggcgaatgcc
    46981 gacgcgctgg cagcgttgct gaaaacgcac ggtctgcatg tcgatgccga agaagtgctc
    47041 acgctggacg gtttctgcaa actgcgtcgt gaactggatg cgcaaccaac tggtttcctc
    47101 gacagccgca ttcgtcgctt ccagtcattt gctgaaatta gcactgaacc cggtcctcac
    47161 tttggcctcg gtctggaagc atacgccacc tggacttcgc cgatccgtaa atatggcgac
    47221 atgatcaacc accgtctgct gaaagcggtt atcaaaggcg aaactgcgac gcgcccacag
    47281 gatgaaatca ctgtccaaat ggccgagcgt cgccgtctca accggatggc agaacgtgat
    47341 gttggcgact ggttatacgc acgcttcctg aaagacaaag ccgggaccga cacccgtttc
    47401 gcggcggaaa ttgtcgatat cagccgtggc ggcatgcgtg tgcgtttggt tgataacggc
    47461 gctatcgcct ttattccggc acctttctta cacgctatgc gcgatgaact ggtttgcagc
    47521 caggaaaacg gcactgtaca aattaaaggt gaaacggttt acaaagtaac tgacgttatt
    47581 gacgtcacca ttgccgaagt ccgcatggaa acccgcagca ttattgcgcg cccggtcgcg
    47641 taatctcctt tcacggccca ttcctcatgg atgggccgtt tatttccccg ctctaccttc
    47701 gttatatcca ggcagatttt ttaactgact ttcgtttgaa aactggcgtt tttccaaaac
    47761 tggattacaa ataaatatat aaaaaaaaca atgcgtttag ccgccgttaa tttcatgtaa
    47821 gtcatgttgt tccttttatt taaccgcgga ctccgctgtt aaccggagga tatgcatcat
    47881 gaaaaccgtt agggagtcca caacgttgta caactttctc ggatcgcaca atccatactg
    47941 gcggttgacg gaaagcagcg atgttttgcg cttttctacc accgaaacca cagaacctga
    48001 tcgtacattg cagttatctg ccgaacaggc tgctcgcatc agggaaatga cggtcatcac
    48061 ctccagcctg atgatgagtc tgaccgtcga tgaaagcgat ctttctgtgc atctggtagg
    48121 acgaaaaatc aataaacggg aatgggctgg caacgcgtct gcatggcatg acacaccggc
    48181 ggttgctcgt gatttatcac acgggctttc ctttgctgag caggtagttt ctgaagcaca
    48241 ttccgcaata gtgattctcg acagccgggg gaatatccaa cgcttcaatc ggttatgtga
    48301 agattacact gggttgaaag aacacgacgt cattggccaa agcgtgttta aactgtttat
    48361 gagccgtcgt gaagctgcgg catccaggcg caataaccgt gtattttttc gaagcggcaa
    48421 tgcatatgaa gtcgaactgt ggataccaac acgtaaaggc cagcggctgt ttctgtttcg
    48481 caataaattt gtccacagcg gcagtggcaa aaacgagatt tttttaatct gttccggcac
    48541 cgacattacc gaagagcgcc gcgctcagga gcgactgcgt attctggcaa ataccgacag
    48601 tatcaccgga ctgccgaatc gtaacgcaat gcaggattta atcgatcacg ctattaatca
    48661 tgcagataac aataaagttg gggttgtgta tcttgatttg gataatttca aaaaggtcaa
    48721 cgacgcctat gggcatttgt ttggtgacca gttattacgc gacgtgtcat tggctatttt
    48781 aagctgtctc gaacatgacc aggtgttggc gcgtccaggt ggcgatgagt ttctggtact
    48841 ggcatccaac acctcacaaa gcgcgctgga agcaatggca tcacgaattt tgacccgctt
    48901 acggctcccc tttcgcattg gtttaattga agtttatacc agctgttcag taggtattgc
    48961 actctctccc gaacatggtt cagacagcac ggctattatt cgtcacgccg acacagcaat
    49021 gtacacagcg aaggaaggcg gacgaggaca attttgcgtt tttaccccag aaatgaatca
    49081 acgggtattt gaatatctct ggctggatac caacttgcgt aaagcactgg aaaacgatca
    49141 gttggttatt cactatcaac cgaaaatcac ctggcgtggc gaagtgcgca gtctggaagc
    49201 actagtacgt tggcagtcac ctgaacgtgg gttgattcca ccgttggact tcatttccta
    49261 cgccgaagag tcagggctaa ttgtgccttt aggccgttgg gtgattctcg atgtcgtacg
    49321 ccaggtggcg aagtggcggg ataaaggtat taacctgcga gtggcggtaa atatttctgc
    49381 acgtcagctc gccgatcaaa ccattttcac cgccctgaaa caggttctcc aggaactcaa
    49441 ttttgaatac tgccctatag atgttgaact gacagagagt tgtctgattg agaatgatga
    49501 actggcactg tctgttattc aacaatttag ccaactaggt gcgcaagtgc atctggatga
    49561 ttttggtacc ggctactctt cactttcgca actggcgcgc tttccgatcg atgccatcaa
    49621 acttgaccag gtttttgttc gagatattca caaacaacct gtctcgcagt cactggtccg
    49681 ggcgatcgtc gctgtggccc aggcattgaa tcttcaggtg atcgccgaag gtgtagagag
    49741 tgcaaaggaa gatgcttttt taaccaagaa cgggatcaat gagcggcaag gatttttgtt
    49801 tgccaaaccg atgcccgccg tcgccttcga acgctggtat aaacgctatc tgaagcgcgc
    49861 ataagtagat gaatatatat acttagcgct atatcccgtc attatatatc gtattcctgg
    49921 atgataatta gccgttgctg ttaaacactt ctcttttagc ggcaattttt gcattattca
    49981 ttttccactt ttgctataag gatgtccttg atgtctgaat ttgatgccca gcgcgttgcc
    50041 gaacgtattg atattgtgtt agatatcctt gtggcaggcg attaccactc tgcgatccat
    50101 aatctcgaga tacttaaagc agaactcttg cgccaggttg ctgaatctac gccagacatc
    50161 ccgaaggcac cgtgggagat ttaacaatcg tgccttcagg ttttcctatt tgaggatgcc
    50221 cggcgcgtca gtattttttt atttagtatt ataacgttat aagaattaca gcgatgaatt
    50281 cccgacaaca aactattcta cagatggtca ttgaccaggg tcaggttagc gtaaccgatc
    50341 tggcaaaagc cactggagtt tctgaagtta ccattcgcca ggatctcaac accctcgaaa
    50401 aactgagtta cctccgccgt gcacatggct ttgcagtttc gcttgatagt gatgacgtcg
    50461 aaacccgtat gatgagcaac tatacgctga agcgtgaact cgccgagttt gccgcgtcac
    50521 tggttcaacc gggcgaaacc atctttatcg aaaatggtag cagcaatgcc ctgctcgccc
    50581 ggactctggg cgaacagaag aaaaatgtca ctatcatcac agtcagcagc tacattgcgc
    50641 atttgctgaa agacgcgcct tgtgaagtca ttttgctcgg tggcgtgtac cagaaaaaaa
    50701 gcgaaagtat ggttggccct ttgacacgcc agtgcatcca gcaggtatat ttcagcaaag
    50761 catttattgg tattgatggc tggcaacctg aaactggatt caccggtcgc gatatgatgc
    50821 gtaccgatgt ggtcaatgcc gtgctggaaa aagagtgcga agcgatagtc ctgactgaca
    50881 gctcgaaatt tggtgctgta cattcatact ccatcggtcc cgttgagcga ttcaatcgcg
    50941 tgattaccga ttcgaaaata cgcgccagcg atctgatgca tcttgagcac agtaaactca
    51001 ccgttcacgt cgttgacatt taaccatttc ccttctctac ggatgatttg cagtttggca
    51061 aatcatccgc tctaagatga ttcctggttg ataattaaga ctatttacct gttattaaca
    51121 ctctcaagat ataaaattat tatcagcgat ataacaggaa gtcattatca cctgcgtgat
    51181 ataaccctgc gcgcgagcag atttcacgga ataatttcac caggcttatt cttagctatt
    51241 atagttatag agagcttact tccgtgaatc ataaattcag gagagagtat tatgtttgta
    51301 acgagcaaaa aaatgaccgc ggctgttctg gcaattaccc tggcaatgtc tctgagtgcc
    51361 tgttctaact ggtctaaacg ggaccgcaac accgcaatcg gcgcgggtgc aggggcatta
    51421 ggcggtgcag tactgaccga tggcagtacg ttgggtacat taggtggtgc agctgtcggt
    51481 ggtgttattg gtcaccaggt cggtaaataa tcgtaaataa atgtcgatta tcgactcgca
    51541 attataatac gcttgattct gttcagtacg aaataactga ataatacaca cagtaaaata
    51601 ttcgaaagcc acggatatat ccgtggcttt ttatgttaac cacctgcgag ttttaccttc
    51661 atccctttcg cttcaagtag tgattttaat aaatcacgct tatcgccctg gatttcaata
    51721 actccatctt taactgctcc gccgcagccg cattttttct tcagttccgc tgcaagtttt
    51781 gtcagttcgg catcatcgag atcgacaccg gtaatcaggc aaacgccctt acctttacgt
    51841 ccactggtct gacgctgaat acgcaccaca ccgtcgcctt tagggcgtac aggggccgct
    51901 ttgggctcat caatacgtcc ggtttccgtt gagtagacca gacggctgtt ggaatcactc
    51961 atcatgcact ccgctgtaaa gaggcgttga tcgctttcag cgtctgcgct ggatctaccg
    52021 attgcgttac cgggcgacca atcaccatat aatcaacacc agccgacaac gcctgttctg
    52081 gcgtcataat gcggcgctgg tcaccagctt cactcccctg cggacgaatg cccggcgtaa
    52141 ccagtttgaa ctcctgaccg aatacctgtt taaagcgcac agcttcctga gcagaacaca
    52201 ccacaccatc aaggccacat ttttgcgtca gtgccgccag acgttctgca taatctgcag
    52261 gtgacagtgt catgccaaga tcgaccaggt cgctggcttc catgctggtc aacactgtca
    52321 cagcaatcaa aagcggtgca tctttgccaa acggaaccag tgcctcacgc gctgcggtca
    52381 tcatacgcgc cccaccagag gcatgaacat tcaccatcca cacgcctaag tcagctgcag
    52441 cagcgacagc gtgcgcagca gtgttaggaa tatcgtggaa tttcaggtca agaaagatat
    52501 caaaaccacg ctgttgaagt tcgcgcacaa actgtggccc aaacaatgta aacatctctt
    52561 tgccgacctt cagacgacaa tcgcgtgggt cgatcttgtc gacaaaggac agcgcgtcat
    52621 cacgattatg ataatcaagg gcaacaacca caggagaatt cgtaacagcg cgggaagaag
    52681 atgaagcagt taacgtcatg accagacctt cttgatgatg ggcaccggaa gtggcgcgaa
    52741 acaggtaaac ggcgagcatt ctacccgtgg aaataaaaaa ttaacagttg cgatttttcc
    52801 cctggcaggc tttttctgcg ccgtcgttgc cggagcacaa tggaacatca ataactagta
    52861 tgttgtaact aaagtcggat ttttttaaaa attacaggcc atcaagaccg cgaatcggtt
    52921 taatggttga ccaggcccga caagacggac aatgccagta gagggtgtat gcggtaaaac
    52981 cacatttctg gcagcgataa cgaggcttac tacgtacctt ttcgccaacc atgtcacgca
    53041 gcaccatcag gctctctttg gcacgccctt cttccgcttc atttaagtgg taatccatta
    53101 acttatggaa cacacgcatg gtcggatgac gctgaagctg gcgcgtaata tagacctgtg
    53161 cggcctcact accgtcgcgc gcttcgatga tatcagcaag catcaattca gcatcggcac
    53221 cggtgttctc ttccaccgcg cgttgcagga attctgccca ttcggcagtt ttacccaact
    53281 gctgatagca ggtttgcaac atttccagcg tttcgctgac cagttctctg tcctgggata
    53341 tcacgcgttg caaactttcg acggctttgg cgtattctcc tttcgccata aacacgcgtc
    53401 ccatcatgat ggatacgcgg gcgctgtttt tatctgccgc cgcccctttt tttagcaagg
    53461 tcatggcacg atcgagatcg tcgctggcca tatgctgcag ggctaactca cagtagaaat
    53521 gggcaatttc gacgcgctgt ttatctttac ccagcttcac caggcgttcg gcaacatcaa
    53581 ttgctttctg ccactcactg gtagcctggt agatttgtag caactgttgc agcgcgccaa
    53641 tgcggaagtc agtttcatcg gtcagctgat tgaacatgtc ttccgcgcgg tcgtataacc
    53701 cggcggccat gtaatcacgc cccagttgtt gaatcgccaa cagacgctgt tcataggtca
    53761 gcgaggcgct ttccattagg gtctgatgga tgcgaatagc gcgatcaact tcgccacgcg
    53821 aacggaacag gtttccgagc gtaaggtggg cttcaacggt gcctgtatcc tctttaagca
    53881 tatcgagaaa caggtctacc gctttatcct gttgattact aagcaggaag ttaacccccg
    53941 ctacgtaatc acgcgacaag cggttggctt catcttgctt gttttgttgc gcacttctgc
    54001 ggcccatata ccagccatag gcagcggcta caggcaaaag cagaaacaac aactccagca
    54061 tagaaagtta ttccttcgcc gctgacgagt gcggcactac agccacgtca gtcgcgggtg
    54121 aaagctggtt ttccagtcgc tttattttac gttcagcgcg cgccagggaa acacgaactc
    54181 gcagccagaa caggccacaa atcaaccaac cgatagcaaa ccccgcagca aacaataccg
    54241 ccagcaatgt ggaaatacgg tactcccctt gcgctaacag ataattaaac gtcacctgtt
    54301 gatcgttctg cgcacccaac gtgaccgaaa tcacgaagat cgctaacacc agtaagaaaa
    54361 tgagtaaata tttcacatta cttcccgtta tgtggttaga gcgaataaat tatggtcaac
    54421 ttaccgcaat agccctttaa agtaccattt tcgcgccgtc tgcgaaatgg catccacgcg
    54481 cattgttgat gatttaaggg ctaaattcgg aaaatcaacc agcgttaact ttcttgttct
    54541 cgttgcgcta tttcgcgatt ttcttccgca ggtggtgtta atggcccaca aattcgttgc
    54601 gcaagccacg ttgccaccgc caccagcgcc cacgaaatca acgtagctac taccagatcg
    54661 cgtggccaat gcatcccgag cagcaggcgg cttcccatga ctcccgttgc ccagaccagc
    54721 aagatagcaa tggttaacgt tcgccgacgc ggccacagca aaccaacggc cagcagtgcc
    54781 caactggcag caaacatcgt gtgaccggaa ggaaaggcaa accccgtctc tttctgccag
    54841 tgtgaacgca aatattgtgg gatatttttc tcttcagcca actgttcttt cactaaattt
    54901 ccgcgttctg ctcgctttaa agtgtagaac tcatcaaccg gaatatgatg tgttttttcc
    54961 agccagataa caaaaggtcg tggttcctgg actttgtctt tgatccagga tttaacgcct
    55021 tgtcccacaa ggattgcggc cgccagaatg gcaaataata caaaggcagc cttaatgcga
    55081 aaacgcagac accagagaaa ccagccgaat aaaatcaaat gtgtaatgac gccccagggc
    55141 tgggtgacag tttcagtaac ccaaaaagcc gcttttagta gccaactttg ttctccaggt
    55201 tgccaacgcc agccagaaat ccatacggct actggcatga caagcaatag tgcagctccc
    55261 actgcggtac gtctggcaat cgaacgcatg gcctctcctt tttgataagt cccacaatca
    55321 taactgaaaa cgccagttcc aggaaaaatt gacagatttg tgccattccg tgaacgatcg
    55381 acgcgtcgtg attaggtgaa ccccttctcg ttatggcaaa ataagccaat acagaaccag
    55441 cattatctgg agaatttcat gcagcttaaa cgtgtggcag aagccaaact gccaacccca
    55501 tggggcgatt tcctgatggt gggatttgaa gaactggcaa ccggacacga tcatgtcgcg
    55561 ctagtctatg gcgatatttc cgggcatacc ccggtacttg cgcgcgtcca ttccgaatgt
    55621 ctgaccggtg acgccctgtt cagcttgcgc tgcgattgtg gcttccagct cgaagcggca
    55681 ttgacgcaaa ttgccgagga aggccgtggt attttgctgt atcaccgtca ggaaggtcgt
    55741 aacattggtc tgctgaataa aatccgcgct tacgcactgc aggatcaagg ttacgatacc
    55801 gtagaggcta accaccagtt aggcttcgcc gctgatgagc gcgacttcac tctttgcgct
    55861 gatatgttca aactccttgg cgtcaatgaa gtccgcttgt taaccaataa cccgaaaaaa
    55921 gtcgaaattc tgaccgaagc agggattaat attgttgaac gcgtaccatt gattgtaggt
    55981 cgtaacccca ataacgaaca ttatctcgat accaaagccg agaaaatggg ccatttgctg
    56041 aacaaataac cctcttgcat tgtgtaatta gtttgcttgc cggaagcaaa ataaccggca
    56101 agcaaatact tgttacttca acatattacg aatgacataa tgcaaaatgc cgtcgttctg
    56161 gtagtaggtc aactccgtcg cggtgtcgat acgacaacgg cagggtacga cctcctggct
    56221 accatccgcg cgcgtaagcg tcaccggaac cgtcgcaccg ggttgtagat tttgcagatc
    56281 gccaatatca atcttctctt ccccggttag ttctaacgtt ttacgcgtta cgccttgcgg
    56341 aaattccagc ggcaggatgc ccatgccaat taaattcgaa cggtgaattc gttcaaacga
    56401 ttcggcaatc accacacgaa ttccaagcag acgaggacct tttgccgccc agtcacggct
    56461 tgagcctgat ccatactctt tcccggcaat caccgccagc ggcgtttgtt cctgcttata
    56521 gcgcatcgca gcatcataaa tagagactac gtcgctgtca ggtaaatgcc gcgtcatccc
    56581 cccttcaacg ccaggcacca tttcattacg gatgcgaata ttggcgaagg tgccgcgcat
    56641 catcacttca tggttaccac gccgcgaacc gtaggagtta aagtcttttc gctcaacacc
    56701 ccgaccttgt agatatcgac ccgctgggct gtcgggctta atactgcccg ccggagagat
    56761 atggtcagtg gtgactgaat cccccagcat tgcgaggatc cgcgcaccgt gaatatcttc
    56821 cactggtgct ggtgttgcct gcatttcatc aaagaaaggc gataagcgaa tataggttga
    56881 gtcctcctgc caaccgtagg tatcggatcg tgtgacgtta atttccttcc actctgctgt
    56941 gccttcaaaa acttctgcgt actctttgcg gaacatttct gtggagactt gttctaccgc
    57001 acgggcaatt tcttgtgccg atggccagat atctttcaga taaaccgggt cgcctttacg
    57061 atcatgacca ataggctcag cagccaggtt gatattcata tttcccgcca gcgcataggc
    57121 aaccaccagc ggcggcgagg ccagccagtt agttttaacc agcggatgga tacggccttc
    57181 aaagttacgg ttgccggaca gcactgcacc gacggttaaa tcgccttttt tgattgccgt
    57241 ttcgatagga tcgggcagcg gcccagagtt accaatacag gtggtacaac cgtatcccac
    57301 aaggttaaac cccagttcgt cgagatacgg tgtcagtttc gcttttgcca gataatcaga
    57361 aacgactttc gaacccggtg ccagcgacgc tttgacccac ggttgccgct tgaggcccag
    57421 agttacggct tttttcgcca gcaagcctgc ggccatcagc acacttgggt tagaggtgtt
    57481 ggtgcacgag gttatcgcag caatgaccac agcgccatca ggtaactgat actgatgtcc
    57541 gttcataaca taatcgaccg gctggcgatc tttatgcgtg gcattcactt ccagttcgtt
    57601 actggcggca aatgcttttg gtacatcggg cagtgcaacg cgatcctgtg ggcgttttgg
    57661 ccctgccagg ctcgcttcaa cgtcattcat atccagttct aacgtactgg taaaaatcgg
    57721 ttcatcgccc gggttacgcc acatgccctg cgctttggca tatttttcga ccaactcaac
    57781 ctgatcttcg ctgcgcccgc ttaaacgcat gtaatcgagg gttacagcat cgattgggaa
    57841 gaagccacag gtggcaccat attctggcga catattggca atggtggcgc gatccgccag
    57901 cggtagtgaa tccagaccat caccataaaa ttcgacgaat ttccccacca cgccatgttt
    57961 gcgcagcatt tgggtaacag tgagaaccag gtctgtggcg gtaatacctt cacgtaattt
    58021 accggtaagt ttaaagccca ctacgtccgg gataagcatg gaaaccggct ggcctaacat
    58081 tgcggcttct gcttcgatcc cacccacgcc ccaccccagc acgccaaggc cgttgatcat
    58141 agtggtgtgc gagtcagtac caacgagtgt atccggataa gcaatccatt caccgtcctg
    58201 caattcactc cacactgctt tgccgagata ttcgaggtta acctgatggc aaatgcctgt
    58261 gcctggcggc acgacgctaa accgactgaa cgcttgcttt ccccatttca ggaacacata
    58321 acgttcgtgg ttgcgctcca tttccaggcg tacgttttct tcaaatgcct catcatcacc
    58381 aaaacgatcg acggtcaccg agtggtcaat gaccaggtcg accggtgaga gcgggttaac
    58441 ctttgcagta tcgccgccga ggcgtttaac cgcttcgcgc attgccgcca gatcaacaac
    58501 ggcaggtacg ccggtaaagt cctgcatcag cacccttgcc gggcggtagg caatttcacg
    58561 gtcagcatgg gcatttttca gccatcctgc cagcgcgtgg atatcctctt cggtaaccga
    58621 gttaccatcc tgccagcgca gcaggttttc gagcaaaact ttgagtgact tgggcagacg
    58681 ggtgatatcg cccagtgatt tagcagcaag cggcaggctg tagtagtggt aagttttatc
    58741 tttggcctgc aacgtgtcct tactggcttc tcgtagggtt gacgacatag ctcctcctta
    58801 atgacagggt tgcgaagccc tgattctctt cagggtgaat attaaagata acacaaacat
    58861 cgcgtaacga tttgataaca acccaaattg ataaaagagg agaaaccttg tggcaaagca
    58921 aatgacaacc ccgccgcagc ggggtcaagg atcagtgagt aatgatccag aggagttcgg
    58981 cccagaagag catcgacaga gaaaacgcgc ccatccagga ccaatatttc atatttgtgt
    59041 tgatcatagg ctacaccctt aacggttatt actttgtgtt gagataactc aacctgttat
    59101 tgctgcggtg tattaaaagc cctgtaattc attaaaaatg ggtaataaga atgatgataa
    59161 ttattgtttc ggctgatcat cttcagcaaa cagttctata aaggcttgct gctgcgatgt
    59221 tagttgccag tcgtctggaa taacgacgga acattcccgt ttcgtttctg tttcattgtc
    59281 actatgctgg cataatggtg acgactccag ttgctcctga ccgaccatag ttatccccac
    59341 actttccaaa tcagcagtgc gacaaccacc cagaaaagtg cagaaccaag aaaaaccgcc
    59401 agccaggctt tacgttttaa ttcaggatcg cggcggggtt cctgatttcc agaaggcatt
    59461 gctaacctca tataatcgct atcacttatc agcaagacgt tgaacgattg gtgtaattaa
    59521 ttatacgatg agacaaatcc taaagaaact ttaggcaaaa atccagcgaa tttacgaatc
    59581 gtctccagtg aataattcaa tcttttgacc tgaaatagat atttgaaaac tggagtttgc
    59641 gaggcgggta attagacact actttacagg taatttaccc tataaatatg agggataaaa
//
LOCUS       DAPHDW010000010        59224 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.010, whole genome shotgun sequence.
ACCESSION   DAPHDW010000010 DAPHDW010000000
VERSION     DAPHDW010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 59224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59224
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.010"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            173..844
                     /locus_tag="RH057_000609"
     CDS             173..844
                     /locus_tag="RH057_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016241930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HDV3127025.1"
                     /translation="MDKVLDSALLSSANKRKGILAIGAHPDDIELGCGASLARLAQKG
                     IYIAAVVMTTGNSGTDGIIDRHEESRNALKILGCHQTIHLNFADTRAHLQLNDMISAL
                     EDIIKNQIPSDVEIMRVYTMHDADRHQDHLAVYQASMVACRTIPQILGYETPSTWLSF
                     MPQVFESVKEEYFTVKLAALKKHKSQERRDYMRHDRLRAVAQFRGQQVNSDLGEGFVI
                     HKMIL"
     gene            854..2050
                     /locus_tag="RH057_000610"
     CDS             854..2050
                     /locus_tag="RH057_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HDV3127026.1"
                     /translation="MKTWIFICMSIAMLLWFLSTLRRKPSQKKGCIDAIIPAYNEGPC
                     LAQSLDNLLRNPYFCRVICVNDGSTDNTEAVMAEVKRKWGDRFVAVTQKNTGKGGALM
                     NGLNYATCDQVFLSDADTYVPPDQDGMGYMLAEIERGADAVGGIPSTALKGAGLLPHI
                     RATVKLPMIVMKRTLQQLLGGAPFIISGACGMFRTDVLRKFGFSDRTKVEDLDLTWTL
                     VANGYRIRQANRCIVYPQECNSPREEWRRWRRWIVGYAVCMRLHKRLLFSRFGIFSIF
                     PMLLVVIYGVGIYLTTWFNEFITTGPHGVVLAMFPLIWIGVVCVIGAFSAWFHRCWLL
                     VPLAPLSVVYVLLAYAIWIIYGLIAFFTGREPQRDKPTRYSALVEASTAYSQPSVTGT
                     EKLSEA"
     gene            2028..2609
                     /locus_tag="RH057_000611"
     CDS             2028..2609
                     /locus_tag="RH057_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV3127027.1"
                     /translation="MKNYLKLNVKEKNIQIADQVIIDETAGEVVIGANTRICHGAVIQ
                     GPVVIGANCLIGNYAFIRPGTIISNGVKIGFATEIKNAVIEAEATIGPQCFIADSVVA
                     NQAYLGAQVRTSNHRLDEQPVSVRTPEGIIATGCDKLGCYIGQRSRLGVQVIILPGRI
                     ISPNTQLGPRVIVERNLPAGTYSLRQELIRTGD"
     gene            2611..3384
                     /locus_tag="RH057_000612"
     CDS             2611..3384
                     /locus_tag="RH057_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiO family outer membrane beta-barrel protein"
                     /protein_id="HDV3127028.1"
                     /translation="MIKRTLLAAAIFSALPAYAGLTSITAGYDFTDYSGDHGNRNLAY
                     AELVAKVENATLLFNLSQGRRDYETEHFNATRGQGAVWYKWNNWLTTRTGIAFADNTP
                     VFARQDFRQDINLALLPKTLFTTGYRYTKYYDDVEVDAWQGGVSLYTGPVITSYRYTH
                     YDSSDAGGSYSNMISVRLNDPRGTGYTQLWLSRGTGAYTYDWTPETRYGSMKSVSLQR
                     IQPLTEQLNLGLTAGKVWYDTPTDDYNGLQLAAHLTWKF"
     gene            3569..3844
                     /gene="frmR"
                     /locus_tag="RH057_000613"
     CDS             3569..3844
                     /gene="frmR"
                     /locus_tag="RH057_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414891.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formaldehyde-responsive transcriptional
                     repressor FrmR"
                     /protein_id="HDV3127029.1"
                     /translation="MPSTPEEKKKVLTRVRRIRGQIDALERSLEGDAECRAILQQIAA
                     VRGAANGLMAEVLESHIRETFDRNDCYSREVSQSVDDTIELVRAYLK"
     gene            3879..4988
                     /gene="frmA"
                     /locus_tag="RH057_000614"
     CDS             3879..4988
                     /gene="frmA"
                     /locus_tag="RH057_000614"
                     /EC_number="1.1.1.284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-(hydroxymethyl)glutathione dehydrogenase"
                     /protein_id="HDV3127030.1"
                     /translation="MKSRAAVAFAPGKPLEIVEIDVAPPKKGEVLIKVTHTGVCHTDA
                     FTLSGDDPEGVFPVVLGHEGAGVVVEVGEGVTSVKPGDHVIPLYTAECGECEFCRSGK
                     TNLCVAVRETQGKGLMPDGTTRFSYNGQPLYHYMGCSTFSEYTVVAEVSLAKINPEAN
                     HEHVCLLGCGVTTGIGAVHNTAKVQPGDSVAVFGLGAIGLAVVQGARQAKAGRIIAID
                     TNPKKFDLARRFGATDCINPNDYDKPIKDVLLDINKWGIDHTFECIGNVNVMRAALES
                     AHRGWGQSVIIGVAGSGQEISTRPFQLVTGRVWKGSAFGGVKGRSQLPGMVEDAMKGD
                     IDLEPFVTHTMSLDEINDAFDLMHEGKSIRTVIRY"
     gene            5081..5914
                     /gene="fghA"
                     /locus_tag="RH057_000615"
     CDS             5081..5914
                     /gene="fghA"
                     /locus_tag="RH057_000615"
                     /EC_number="3.1.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV3127031.1"
                     /translation="MELIEKHASFGGWQNVYRHYSQSLKCEMNVGVYLPPKAANEKLP
                     VLYWLSGLTCNEQNFITKSGMQRYAAEHNIIVVAPDTSPRGSHVADADRYDLGQGAGF
                     YLNATQAPWNEHYKMYDYIRNELPDLVMQHFPATTRKSISGHSMGGLGALVLALRNPD
                     EYVSVSAFSPIVSPSQVPWGQQAFAAYLGENKDAWLDYDPVSLISQGQRVAEIMVDQG
                     LSDDFYAEQLRTPNLEKICQEMNIKTLIRYHEGYDHSYYFVSSFIGEHIAYHANKLNM
                     R"
     gene            complement(6140..6679)
                     /locus_tag="RH057_000616"
     CDS             complement(6140..6679)
                     /locus_tag="RH057_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2058 domain-containing protein"
                     /protein_id="HDV3127032.1"
                     /translation="MAKLTLQEQLLKAGLVTSKKAAKVERTAKKSRVQAREARAAVEE
                     NKKAQLERDKQLSEQQKQAALAKEYKAQVKQLIEMNRITIANGDIGFNFTDGNLIKKI
                     FVDKLTQAQLINGRLAIARLLVDNNSEGEYAIIPASVADKIAQRDASSIVLHSALSAE
                     EQDEDDPYADFKVPDDLMW"
     gene            complement(6781..7992)
                     /gene="mhpT"
                     /locus_tag="RH057_000617"
     CDS             complement(6781..7992)
                     /gene="mhpT"
                     /locus_tag="RH057_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(3-hydroxy-phenyl)propionate transporter MhpT"
                     /protein_id="HDV3127033.1"
                     /translation="MSTRTPSSSSSRLMLTIGLCFLVALMEGLDLQAAGIAAGGIAQA
                     FALDKMQMGWIFSAGILGLLPGALVGGMLADRYGRKRILIGSVALFGLFSLATAIAWD
                     FPSLVFARLMTGVGLGAALPNLIALTSEAAGPRFRGTAVSLMYCGVPIGAALAATLGF
                     AGANLAWQTVFWVGGVVPLILVPLLMRWLPESAVFAGEKQAAPPLRALFAPETATATL
                     LLWLCYFFTLLVVYMLINWLPLLLVEQGFQPSQAAGVMFALQMGAASGTLMLGALMDK
                     LRPVTMSLLIYSGMLASLLALGTVSSFNGMLLAGFVAGLFATGGQSVLYALAPLFYSS
                     QIRATGVGTAVAVGRLGAMSGPLLAGKMLALGTGTVGVMAASAPGILVAGLAVFILMS
                     RRSRMQPCADA"
     gene            complement(8267..9280)
                     /gene="dmpG"
                     /locus_tag="RH057_000618"
     CDS             complement(8267..9280)
                     /gene="dmpG"
                     /locus_tag="RH057_000618"
                     /EC_number="4.1.3.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxovalerate aldolase"
                     /protein_id="HDV3127034.1"
                     /translation="MNGKKLYISDVTLRDGMHAIRHQYSLENVRQIAKALDDARVDSI
                     EVAHGDGLQGSSFNYGFGAHSDLEWIEAAADVVKHAKIATLLLPGIGTIHDLKNAWQA
                     GARVVRVATHCTEADVSAQHIQYARELGMDTVGFLMMSHMTTPENLAKQAKLMEGYGA
                     TCIYVVDSGGAMNMSDIRDRFRALKAELKPETQTGMHAHHNLSLGVANSIAAVEEGCD
                     RIDASLAGMGAGAGNAPLEVFIAAADKLGWQHGTDLYALMDAADDLVRPLQDRPVRVD
                     RETLALGYAGVYSSFLRHCETAAARYGLSAVDILVELGKRRMVGGQEDMIVDVALDLR
                     NNK"
     gene            complement(9277..10227)
                     /gene="mhpF"
                     /locus_tag="RH057_000619"
     CDS             complement(9277..10227)
                     /gene="mhpF"
                     /locus_tag="RH057_000619"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetaldehyde dehydrogenase"
                     /protein_id="HDV3127035.1"
                     /translation="MSKRKVAIIGSGNIGTDLMIKILRHGQHLEMAVMVGIDPQSDGL
                     ARARRMGVATTHEGVIGLMNMPEFADIDIVFDATSAGAHVKNDAALREAKPDIRLIDL
                     TPAAIGPYCVPVVNLEANVDQLNVNMVTCGGQATIPMVAAVSRVARVHYAEIIASIAS
                     KSAGPGTRANIDEFTETTSRAIEVVGGAAKGKAIIVLNPAEPPLMMRDTVYVLSDEAS
                     QDDIEASINEMAEAVQAYVPGYRLKQRVQFEVIPQDKPVNLPGVGQFSGLKTAVWLEV
                     EGAAHYLPAYAGNLDIMTSSALATAEKMAQSLARKAGEAA"
     gene            complement(10224..11033)
                     /gene="mhpD"
                     /locus_tag="RH057_000620"
     CDS             complement(10224..11033)
                     /gene="mhpD"
                     /locus_tag="RH057_000620"
                     /EC_number="4.2.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-4-pentenoate hydratase"
                     /protein_id="HDV3127036.1"
                     /translation="MTKHTLEQLAADLRRAAEQGEAIAPLRDLIGIDNAEAAYAIQHI
                     NVQYDVVQGRRVVGRKVGLTHPKVQQQLGVDQPDFGTLFADMCYGDNEIIPFSRVLQP
                     RIEAEIALVLNRDLPATDITFDELYNAIEWVLPALEVVGSRIRDWSIQFVDTVADNAS
                     CGVYVIGGPAQRPAGLDLKNCAMKMTRNNEEVSSGRGSECLGHPLNAAVWLARKMASL
                     GEPLRAGDIILTGALGPMVAVNAGDRFEAHIEGIGSVAATFSSAAPKGSLS"
     gene            complement(11043..11909)
                     /gene="mhpC"
                     /locus_tag="RH057_000621"
     CDS             complement(11043..11909)
                     /gene="mhpC"
                     /locus_tag="RH057_000621"
                     /EC_number="3.7.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414883.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-6-oxonona-2,4-dienedioate hydrolase"
                     /protein_id="HDV3127037.1"
                     /translation="MSYQPQTEAATSRFLNVEEAGKTLRIHFNDCGQGDETVVLLHGS
                     GPGATGWANFSRNIDPLVEAGYRVILLDCPGWGKSDSIVNSGSRSDLNARILKSVVDQ
                     LDIAKIHLLGNSMGGHSSVAFTLNWPERVGKLVLMGGGTGGMSLFTPMPTEGIKRLNQ
                     LYRQPTIENLKLMMDIFVFDTSDLTDALFEARLNNMLSRRDHLENFVKSLEANPKQFP
                     DFGPRLAEIKAQTLIVWGRNDRFVPMDAGLRLLSGIAGSELHIFRDCGHWAQWEHADA
                     FNQLVLNFLARP"
     gene            complement(11927..12871)
                     /gene="mhpB"
                     /locus_tag="RH057_000622"
     CDS             complement(11927..12871)
                     /gene="mhpB"
                     /locus_tag="RH057_000622"
                     /EC_number="1.13.11.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxyethylcatechol 2,3-dioxygenase"
                     /protein_id="HDV3127038.1"
                     /translation="MHAYLHCLSHSPLVGYVDPAQEVLDEVNGVIASARERIAAFSPE
                     LVVLFAPDHYNGFFYDVMPPFCLGVGATAIGDFGSAAGELPVPVELAEACAHAVMKSG
                     IDLAVSYCMQVDHGFAQPLEFLLGGLDKVPVLPVFINGVATPLPGFQRTRMLGEAIGR
                     FTSTLNKRVLFLGSGGLSHQPPVPELAKADAHMRDRLLGSGKDLPASERELRQQRVIS
                     AAEKFVEDQRTLHPLNPIWDNQFMTLLEQGRIQELDAVSNEELSAIAGKSTHEIKTWV
                     AAFAAISTFGNWRSEGRYYRPIPEWIAGFGSLSARTEN"
     gene            complement(12873..14537)
                     /locus_tag="RH057_000623"
     CDS             complement(12873..14537)
                     /locus_tag="RH057_000623"
                     /EC_number="1.14.13.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-(3-hydroxy-phenyl)propionate/3-hydroxycinnamic acid
                     hydroxylase"
                     /protein_id="HDV3127039.1"
                     /translation="MAIQHPDIQPAVNHSVQVAIAGAGPVGLMMANYLGQMGIDVLVV
                     EKLDKLIDYPRAIGIDDEALRTMQSVGLVENVLPHTTPWHAMRFLTPKGRCFADIQPM
                     TDEFGWPRRNAFIQPQVDAVMLEGLSRFPNVRCLFARELEAFSQHNDEVTLHLKTAEG
                     QRETVKAQWLVACDGGASFVRRTLNVPFEGKTAPNQWIVVDIANDPLSTPHIYLCCDP
                     VRPYVSAALPHAVRRFEFMVMPGETEEQLREPQNMRKLLSKVLPNPDNVELIRQRVYT
                     HNARLAQRFRIDRVLLAGDAAHIMPVWQGQGYNSGMRDAFNLAWKLALVIQGKARDAL
                     LDTYQQERRDHAKAMIDLSVTAGNVLAPPKRWQGTLRDGVSWLLNYLPPVKRYFLEMR
                     FKPMPQYYGGALMREGEAKHSPVGKMFIQPKVTLENGDVTLLDNAIGANFAVIGWGCN
                     PLWGMSDEQIQQWRALGTRFIQVVPEVQIHTAQDNHDGVLRVGDTQGRLRSWFAQHNA
                     SLVVMRPDRFVAATAIPQTLGKTLNKLASVMTLTRPDADVSVEKVA"
     gene            14614..15561
                     /gene="mhpR"
                     /locus_tag="RH057_000624"
     CDS             14614..15561
                     /gene="mhpR"
                     /locus_tag="RH057_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator MhpR"
                     /protein_id="HDV3127040.1"
                     /translation="MIFYCALSIGRVFSATIKTCPNVHQVHNVVLTIEMSINMQNNEQ
                     TEYKTVRGLTRGLMLLNMLNKLDGGASVGLLAELSGLHRTTVRRLLETLQEEGYVRRS
                     PSDDSFRLTIKVRQLSEGFRDEQWISALAAPLLGDLLREVVWPTDVSTLDVDAMVVRE
                     TTHRFSRLSFHRAMVGRRLPLLKTASGLTWLAFCPEQERKELIEMLAARPGDDYQLAR
                     EPLKLQAILARARKEGYGQNYRGWDQEEKIASIAVPLRSEQRVIGCLNLVYMASAMTI
                     EQAAEKHLPALQRVAKQIEEGVESQAILVAGRRSGVHLR"
     gene            15638..16720
                     /gene="lacI"
                     /locus_tag="RH057_000625"
     CDS             15638..16720
                     /gene="lacI"
                     /locus_tag="RH057_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414879.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor LacI"
                     /protein_id="HDV3127041.1"
                     /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE
                     LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS
                     GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII
                     FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG
                     DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT
                     EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT
                     QTASPRALADSLMQLARQVSRLESGQ"
     gene            16843..19917
                     /gene="lacZ"
                     /locus_tag="RH057_000626"
     CDS             16843..19917
                     /gene="lacZ"
                     /locus_tag="RH057_000626"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HDV3127042.1"
                     /translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEAR
                     TDRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYT
                     NVTYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWV
                     GYGQDSRLPSEFDLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHK
                     PTTQISDFHVATRFNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFG
                     GEIIDERGSYADRVTLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFR
                     EVRIENGLLLLNGKPLLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSH
                     YPNHPLWYTLCDRYGLYVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRN
                     HPSVIIWSLGNESGHGANHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVD
                     EDQPFPAVPKWSIKKWLSLPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGG
                     FVWDWVDQSLIKYDENGNPWSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQ
                     QQFFQFRLSGQTIEVTSEYLFRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIE
                     LPELPQPESAGQLWLTVRVVQPNATAWSEAGHISAWQQWRLAENLSVTLPSASHIIPQ
                     LTTSETDFCIELGNKRWQFNRQSGLLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSE
                     ATRIDPNAWVERWKATGHYQAEAALLQCTADTLADAVLITTAHAWQHQGKTLFISRKT
                     YRIDGSGQMAITVDVEVASDTPHPARIGLTCQLAQVAERVNWLGLGPQENYPDRLTAA
                     CFDRWDLPLSDMYTPYVFPSENGLRCGTRELNYGPHQWRGDFQFNISRYSQQQLMETS
                     HRHLLHAEEGTWLNIDGFHMGIGGDDSWSPSVSAEFQLSAGRYHYQLVWCQK"
     gene            19969..21222
                     /gene="lacY"
                     /locus_tag="RH057_000627"
     CDS             19969..21222
                     /gene="lacY"
                     /locus_tag="RH057_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactose permease"
                     /protein_id="HDV3127043.1"
                     /translation="MYYLKNTNFWMFGLFFFFYFFIMGAYFPFFPIWLHDINHISKSD
                     TGIIFAAISLFSLLFQPLFGLLSDKLGLRKYLLWIITGMLVMFAPFFIFIFGPLLQYN
                     ILVGSIVGGIYLGFCFNAGAPAVEAFIEKVSRRSNFEFGRARMFGCVGWALCASIVGI
                     MFTINNQFVFWLGSGCALILAVLLFFAKTDAPSSATVANAVGANHSAFSLKLALELFR
                     QPKLWFLSLYVIGVSCTYDVFDQQFANFFTSFFATGEQGTRVFGYVTTMGELLNASIM
                     FFAPLIINRIGGKNALLLAGTIMSVRIIGSSFATSALEVVILKTLHMFEVPFLLVGCF
                     KYITSQFEVRFSATIYLVCFCFFKQLAMIFMSVLAGNMYESIGFQGAYLVLGLVALGF
                     TLISVFTLSGPGPLSLLRRQVNEVA"
     gene            21288..21899
                     /gene="lacA"
                     /locus_tag="RH057_000628"
     CDS             21288..21899
                     /gene="lacA"
                     /locus_tag="RH057_000628"
                     /EC_number="2.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactoside O-acetyltransferase"
                     /protein_id="HDV3127044.1"
                     /translation="MNMSMTERIKAGKLFTDMCEGLPEKRLRGKTLMYEFNHSHPSEV
                     EKRESLIKEMFATVGENAWIEPPVYFSYGSNIHIGRNFYANFNLTIVDDYTVTIGDNV
                     LIAPNVTLSVTGHPVHHELRKNGEMYSFPITIGNNVWIGSHVVINPGVTIGDNSVIGA
                     GSVVIKDIPPNVVAAGVPCRVIREINDRDKQYYFRDYKVESSV"
     gene            complement(22002..23183)
                     /gene="cynX"
                     /locus_tag="RH057_000629"
     CDS             complement(22002..23183)
                     /gene="cynX"
                     /locus_tag="RH057_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter CynX"
                     /protein_id="HDV3127045.1"
                     /translation="MRNHQKTFTMLLVLVLIGLNMRPLLTSVGPLLPQLRQASGMSFS
                     VAALLTALPVVTMGGLALAGSWLHQHVSERRSVAISLLLIAVGALMRELYPQSVLLLS
                     SALLGGVGIGIIQAVMPSVIKRRFQQRTPLVMGLWSAALMGGGGLGAAITPWLVQHSE
                     TWYQTLAWWALPAVVALFAWWWQSAREVASSHKTTTTPVRVVFTPRAWTLGVYFGLIN
                     GGYASLIAWLPAFYIEIGASAQYSGSLLALMTLGQAAGALLMPAMARHQDRRKLLMLA
                     LVLQLVGFCGFIWLPLQLPVLWAMVCGLGLGGAFPLCLLLALDHSAQPAIAGKLVAFM
                     QGIGFIIAGLAPWFSGVLRSISGNYLMDWAFHALCVVGLMIITLRFAPARFPQLWVKE
                     A"
     gene            complement(23189..23659)
                     /gene="cynS"
                     /locus_tag="RH057_000630"
     CDS             complement(23189..23659)
                     /gene="cynS"
                     /locus_tag="RH057_000630"
                     /EC_number="4.2.1.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyanase"
                     /protein_id="HDV3127046.1"
                     /translation="MIQSQINRNIRLDLADAILLSKAKKDLSFAEIADGTGLAEAFVT
                     AALLGQQALPADAARLVGAKLDLDEDAILLLQMIPLRGCIDDRIPTDPTMYRFYEMLQ
                     VYGTTLKALVHEKFGDGIISAINFKLDVKKVADPEGGERAVITLDGKYLPTKPF"
     gene            complement(23690..24349)
                     /gene="cynT"
                     /locus_tag="RH057_000631"
     CDS             complement(23690..24349)
                     /gene="cynT"
                     /locus_tag="RH057_000631"
                     /EC_number="4.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase CynT"
                     /protein_id="HDV3127047.1"
                     /translation="MKEIIDGFLKFQREAFPKREALFKQLATQQSPRTLFISCSDSRL
                     VPELVTQREPGDLFVIRNAGNIVPSYGPEPGGVSASVEYAVAALRVSDIVICGHSNCG
                     AMTAIASCQCMDHMPAVSHWLRYADSARVVNEARPHSDLPSKAAAMVRENVIAQLANL
                     QTHPSVRLALEEGRIALHGWVYDIESGSIAAFDGATRQFVPLAANPRVCAIPLRQPTA
                     A"
     gene            24459..25358
                     /gene="cynR"
                     /locus_tag="RH057_000632"
     CDS             24459..25358
                     /gene="cynR"
                     /locus_tag="RH057_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CynR"
                     /protein_id="HDV3127048.1"
                     /translation="MLSRHINYFLAVAEHGSFTRAASALHVSQPALSQQIRQLEESLG
                     VPLFDRSGRTIRLTDAGEVWRQYASRALQELGAGKRAIHDVADLTRGSLRIAVTPTFT
                     SYFIGPLMADFYARYPGITLQLQEMSQEKIEDLLCRDELDVGIAFAPVHSPELEAIPL
                     LTESLALVVAQHHPLAACEQVALSRLHDEKLVLLSAEFATREQIDHYCEKAGLHPQVV
                     IEANSISAVLELIRRTSLSTLLPAAIATQHDGLKAISLAPPLLERTAVLLRRKNSWQT
                     AAAKAFLHMALEECADVGENESR"
     gene            complement(25398..26681)
                     /gene="codA"
                     /locus_tag="RH057_000633"
     CDS             complement(25398..26681)
                     /gene="codA"
                     /locus_tag="RH057_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine/isoguanine deaminase"
                     /protein_id="HDV3127049.1"
                     /translation="MSNNALQTIINARLPGKEGLWQIHLQDGKISAIDAQSGVMPITE
                     NSLDAEQGLVIPPFVEPHIHLDTTQTAGQPNWNQSGTLFEGIERWAERKALLTHDDVK
                     QRAWQTLKWQIANGIQHVRTHVDVSDATLTALKAMLEVKQEVAPWIDLQIVAFPQEGI
                     LSYPNGEALLEEALRLGADVVGAIPHFEFTREYGVESLHKTFALAQKYDRLIDVHCDE
                     IDDEQSRFVETVAALAHREGMGARVTASHTTAMHSYNGAYTSRLFRLLKMSGINFVAN
                     PLVNIHLQGRFDTYPKRRGITRVKEMLESGINVCFGHDDVFDPWYPLGTANMLQVLHM
                     GLHVCQLMGYGQINDGLNLITHHSARTLNLQDYGIAAGNSANLIILPAENGFDALRRQ
                     VPVRYSVRGGKVIASTQPAQTTVYLEQPEAIDYKR"
     gene            complement(26671..27930)
                     /gene="codB"
                     /locus_tag="RH057_000634"
     CDS             complement(26671..27930)
                     /gene="codB"
                     /locus_tag="RH057_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosine permease"
                     /protein_id="HDV3127050.1"
                     /translation="MSQDNNFSQGPVPQSARKGVLALTFVMLGLTFFSASMWTGGTLG
                     TGLSYHDFFLAVLIGNLLLGIYTSFLGYIGAKTGLTTHLLARFSFGVKGSWLPSLLLG
                     GTQVGWFGVGVAMFAIPVGKATGLDINLLIAVSGLLMTVTVFFGISALTVLSVIAVPA
                     IACLGGYSVWLAVNGMGGLDALKAVVPAQPLDFNVALALVVGSFISAGTLTADFVRFG
                     RNAKLAVLVAMVAFFLGNSLMFIFGAAGAAALGMADISDVMIAQGLLLPAIVVLGLNI
                     WTTNDNALYASGLGFANITGMSSKTLSVINGIIGTVCALWLYNNFVGWLTFLSAAIPP
                     VGGVIIADYLMNRRRYEHFATTRMMSVNWVAILAVALGIAAGHWLPGIVPVNAVLGGA
                     LSYLILNPILNRKTTAAMTHVEVNSVE"
     gene            complement(28167..30053)
                     /gene="prpE"
                     /locus_tag="RH057_000635"
     CDS             complement(28167..30053)
                     /gene="prpE"
                     /locus_tag="RH057_000635"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate--CoA ligase"
                     /protein_id="HDV3127051.1"
                     /translation="MSFSEFYQRSINEPEQFWAEQARRIDWQTPFTQTLDHSNPPFAR
                     WFCEGRTNLCHNAIDRWLEKQPEALALIAVSSETEEERTFTFRQLHDEVNAVASMLRS
                     LGVQRGDRVLVYMPMIAEAHITLLACARIGAIHSVVFGGFASHSVAARIDDAKPVLIV
                     SADAGARGGKIIPYKKLLDDAISQAQHQPRHVLLVDRGLAKMARVSGRDVDFASLRYQ
                     HIGARVPVAWLESNETSCILYTSGTTGKPKGVQRDVGGYAVALATSMDTIFGGKAGSV
                     FFCASDIGWVVGHSYIVYAPLLAGMATIVYEGLPTWPDCGVWWKIVEKYQVSRMFSAP
                     TAIRVLKKFPTAEIRKHDLSSLEVLYLAGEPLDEPTASWVSNTLDVPVIDNYWQTESG
                     WPIMAIARGLDDRPTRLGSPGVPMYGYNVQLLNEVTGEPCGVNEKGMLVVEGPLPPGC
                     IQTIWGDDDRFVKTYWSLFSRPVYATFDWGIRDADGYHFILGRTDDVINVAGHRLGTR
                     EIEESISSHPGVAEVAVVGVKDALKGQVAVAFVIPKESDSLEDRDVAHSQEKAIMALV
                     DSQIGNFGRPAHVWFVSQLPKTRSGKMLRRTIQAICEGRDPGDLTTIDDPASLDQIRQ
                     AMEE"
     gene            complement(30093..31544)
                     /gene="prpD"
                     /locus_tag="RH057_000636"
     CDS             complement(30093..31544)
                     /gene="prpD"
                     /locus_tag="RH057_000636"
                     /EC_number="4.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate dehydratase"
                     /protein_id="HDV3127052.1"
                     /translation="MSAQINNIRPEFDREIVDIVDYVMNYEISSKVAYDTAHYCLLDT
                     LGCGLEALEYPACKKLLGPIVPGTVVPNGVRVPGTQFQLDPVQAAFNIGAMIRWLDFN
                     DTWLAAEWGHPSDNLGGILATADWLSRNAVASGKAPLTMKQVLTGMIKAHEIQGCIAL
                     ENSFNRVGLDHVLLVKVASTAVVAEMLGLTREEILNAVSLAWVDGQSLRTYRHAPNTG
                     TRKSWAAGDATSRAVRLALMAKTGEMGYPSALTAPVWGFYDVSFKGESFRFQRPYGSY
                     VMENVLFKISFPAEFHSQTAVEAAMTLYEQMQAAGKTAADIEKVTIRTHEACIRIIDK
                     KGPLNNPADRDHCIQYMVAIPLLFGRLTAADYEDNVAQDKRIDALREKINCFEDPAFT
                     ADYHDPEKRAIANAITLEFTDGTRFEEVVVEYPIGHARRRQDGIPKLVDKFKINLARQ
                     FPTRQQQRILEVSLDRTRLEQMPVNEYLDLYVI"
     gene            complement(31578..32747)
                     /gene="prpC"
                     /locus_tag="RH057_000637"
     CDS             complement(31578..32747)
                     /gene="prpC"
                     /locus_tag="RH057_000637"
                     /EC_number="2.3.3.16"
                     /EC_number="2.3.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-methylcitrate synthase"
                     /protein_id="HDV3127053.1"
                     /translation="MSDTTILQNSTHVIKPKKSVALSGVPAGNTALCTVGKSGNDLHY
                     RGYDILDLAEHCEFEEVAHLLIHGKLPTRDELAAYKTKLKALRGLPANVRTVLEALPA
                     ASHPMDVMRTGVSALGCTLPEKEGHTVSGARDIADKLLASLSSILLYWYHYSHNGERI
                     QPETDDDSIGGHFLHLLHGEKPSQSWEKAMHISLVLYAEHEFNASTFTSRVIAGTGSD
                     MYSAIIGAIGALRGPKHGGANEVSLEIQQRYETPDEAEADIRKRVESKEVVIGFGHPV
                     YTIADPRHQVIKRVAKQLSQEGGSLKMYNIADRLETVMWESKKMFPNLDWFSAVSYNM
                     MGVPTEMFTPLFVIARVTGWAAHIIEQRQDNKIIRPSANYVGPEDRQFVALDKRQ"
     gene            complement(32792..33682)
                     /gene="prpB"
                     /locus_tag="RH057_000638"
     CDS             complement(32792..33682)
                     /gene="prpB"
                     /locus_tag="RH057_000638"
                     /EC_number="4.1.3.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylisocitrate lyase"
                     /protein_id="HDV3127054.1"
                     /translation="MSLHSPGKAFRAALTKENPLQIVGTINANHALLAQRAGYQAIYL
                     SGGGVAAGSLGLPDLGISTLDDVLTDIRRITDVCSLPLLVDADIGFGSSAFNVARTVK
                     SMIKAGAAGLHIEDQVGAKRCGHRPNKAIVSKEEMVDRIRAAVDAKTDPDFVIMARTD
                     ALAVEGLDAAIERAQAYVEAGAEMLFPEAITELAMYRQFADAVQVPILANITEFGATP
                     LFTTDELRSAHVAMALYPLSAFRAMNRAAEHVYNVLRQEGTQKSVIDTMQTRNELYES
                     INYYQYEEKLDDLFARSQVK"
     gene            33921..35507
                     /gene="prpR"
                     /locus_tag="RH057_000639"
     CDS             33921..35507
                     /gene="prpR"
                     /locus_tag="RH057_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate catabolism operon regulatory protein
                     PrpR"
                     /protein_id="HDV3127055.1"
                     /translation="MAHPPRLNDDKPVIWTVSVTRLFELFRDISLEFDHLANITPIQL
                     GFEKAVTYIRKKLANERCDAIIAAGSNGAYLKSRLSVPVILIKPSGYDVLQALAKAGK
                     LTSSIGVVTYQETIPALVAFQKTFNLRLDQRSYITEEDARGQINELKANGTEAVVGAG
                     LITDLAEEAGMTGIFIYSAATVRQAFSDALDMTRMSLRHNTHDATRNALRTRYVLGDM
                     LGQSPQMEQVRQTILLYARSSAAVLIEGETGTGKELAAQAIHREYFARHDARQGKKSH
                     PFVAVNCGAIAESLLEAELFGYEEGAFTGSRRGGRAGLFEIAHGGTLFLDEIGEMPLP
                     LQTRLLRVLEEKEVTRVGGHQPVPVDVRVISATHCNLEEDMRQGQFRRDLFYRLSILR
                     LQLPPLRERVADILPLAESFLKVSLAALSAPFSAALRQGLQASETVLVHYDWPGNIRE
                     LRNMMERLALFLSVEPTPDLTPQFLQLLLPELARESAKTPAPRLLTPQQALEKFNGDK
                     TAAANYLGISRTTFWRRLKS"
     gene            complement(35605..35880)
                     /locus_tag="RH057_000640"
     CDS             complement(35605..35880)
                     /locus_tag="RH057_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3127056.1"
                     /translation="MKIISKMLVGALALAVTNVYAAELMTKAEFEKVESQYEKIGDIS
                     TSNEMSTADAKEDLIKKADEKGADVLVLTSGQTDNKIHGTANIYKKK"
     gene            36027..36698
                     /locus_tag="RH057_000641"
     CDS             36027..36698
                     /locus_tag="RH057_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HDV3127057.1"
                     /translation="MMKLLHLFMDEITMDPLHAVYLTVGLFVITFFNPGANLFVVVQT
                     SLASGRRAGVLTGLGVALGDAFYSGLGLFGLATLITQCEEIFSLIRIVGGAYLLWFAW
                     CSMRRQSTPQMSTLQQPISAPWYVFFRRGLITDLSNPQTVLFFISIFSVTLNAETPTW
                     ARLMAWAGIVLASVIWRVFLSQAFSLPAVRRAYGRMQRVASRVIGAIIGVFALRLIYE
                     GVTQR"
     gene            complement(36715..36960)
                     /locus_tag="RH057_000642"
     CDS             complement(36715..36960)
                     /locus_tag="RH057_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127058.1"
                     /translation="MAVQLFKTLLNQIPLLSSLQSGTLPLFGYSGRVRPMKKAHTGES
                     GLKWEAKDSSKLIGNKDHALRGLSSPMAGIRQIRLIT"
     gene            complement(37372..38187)
                     /locus_tag="RH057_000643"
     CDS             complement(37372..38187)
                     /locus_tag="RH057_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127059.1"
                     /translation="MISLKAPHNNLMPYTQQSILNTVKNNQLPEDIKSSLVSCVDIFK
                     VLIKQYYDYPYDCRDDLVDDDKLIHLMAAVRDCEWSDDNALTINVQFNDFPGFYDWMD
                     YPDHPVKFVFRILENQKGTVWVYDQDDAFLDIKANVQAGRFTGLKKLVQFIDSVRTDC
                     KCILLEHHMPLLRIFPKGKECMRVEKWLREMSSIPETDAPIKQALAHGLLLHLKNIYP
                     VFPESLVMLLLSVLDVKTYRDDARLNEWISNRVQELGDRYYPVNKHVKIRYTL"
     gene            complement(38430..39479)
                     /gene="yahK"
                     /locus_tag="RH057_000644"
     CDS             complement(38430..39479)
                     /gene="yahK"
                     /locus_tag="RH057_000644"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase YahK"
                     /protein_id="HDV3127060.1"
                     /translation="MKIKAVGAYSAKQPLEPMDITRREPGPNDVKIEIAYCGVCHSDL
                     HQVRSEWAGTVYPCVPGHEIVGRVVAVGDQVEKHAPGDLVGVGCIVDSCKHCEECEDG
                     LENYCDHMTGTYNSPTPDEPGHTLGGYSQQIVVHERYVLRIRHPQEQLAAVAPLLCAG
                     ITTYSPLRHWQAGPGKKVGVVGIGGLGHMGIKLAHAMGAHVVAFTTSEAKREAAKALG
                     ADEVVNSRNADEMAAHLKSFDFILNTVAAPHNLDDFTTLLKRDGTMTLVGAPATPHKS
                     PEVFNLIMKRRAIAGSMIGGIPETQEMLDFCAEHGIVADIEMIRADQINEAYERMLRG
                     DVKYRFVIDNRTLTD"
     gene            complement(39566..40522)
                     /locus_tag="RH057_000645"
     CDS             complement(39566..40522)
                     /locus_tag="RH057_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3127061.1"
                     /translation="MKKSWRNNVEFYLIGLLVLTVAAFSITMPEIFWSISNFQSVASQ
                     MPVLGILALAMAVTMLCGGINLSIIATANACSLVMAWVATQYPPGIATVVATLLAGAG
                     AAVIIGLCNGVLIAGIRVSPILATLGMMTLLKGINILVTGGSAIANYPSWVLWLNHAQ
                     WFGIPLPMWLFAAVALGLWILLEKTPLGKAIYLIGSNERATLYSGINTRRVLIWVYVI
                     SALLCAVAAFLMMSKLNSAKASYGESYLLVSILAAVLGGVNPDGGSGRIIGMVLALFL
                     LQIIESGFNILGISPYLTMALWGTLLLCFIQVRGMLGLDRVV"
     gene            complement(40519..41490)
                     /locus_tag="RH057_000646"
     CDS             complement(40519..41490)
                     /locus_tag="RH057_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3127062.1"
                     /translation="MAELKKRHEFWLALLIVVLFVGLAWRSDEFLTFGNLYDLANNYA
                     MLTILACGLFVVLISGGIDISFPAMTIIAQYGMVLLLQKIGGNFAVAFALAGCIGILL
                     GLINALLVNRLRVPSIIITISTLNIFYGLLLWLSKGVWLYDFPPWFEQGVMLFKYTDA
                     DGYDYGLGLPLIAMITVVLLTAFIMNFTSVGRKIYALGGNRESASRIGFSVLKLQLFV
                     YGYMGLMSGAAGVVQSWTVMTVAPDSLLGYELTVLAAVVLGGTSLLGGRGTLTGTLLG
                     VVLLAVMQNGLNLLGVSSYWQTLITGIIIVASISATAWSQHQNRSLL"
     gene            complement(41483..42967)
                     /locus_tag="RH057_000647"
     CDS             complement(41483..42967)
                     /locus_tag="RH057_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3127063.1"
                     /translation="METFLSLRHINKTFHATRALRDVSLDFMSGEVHCLAGQNGCGKS
                     TLIKIMSGVYRPDEGAEITLGGKNWSKLTPAASVAQGIQVIYQDLSLFPNLSVWENIA
                     VNHYHHGLFVNRRRLREVAQAAMTSINVTLPLDTLVSELSIARCQLVAICRALAQDAR
                     LIVMDEPTASLTHQEVQGLLQVVHQLRERGICVVFVSHRLEEVMEVSDRISVLKDGEL
                     VGTFPAAEMTTKQLGFLMTGQEFEYQVRELWQGKSSTPVLEVRNLSRHGEYLNINLRV
                     EAGEVVSIVGLLGAGRTELCLSLFGMTRPDAGEILINGQPVTLHSNQDAIRHGIGYVS
                     EDRMSRGLVMAQSIEDNIISTVFHKVKDRFGFLSETKVRDLVDRLIKALTIKVSDPHL
                     PVNTLSGGNAQRVSIAKWLAIGPRLLILDSPTVGVDIANKAGIYGIISDLAAHGIAVL
                     MICDEIEEAWYQSHRILVMQKGQITHSFLPDSSSQARIAEVVNG"
     gene            complement(43016..43999)
                     /locus_tag="RH057_000648"
     CDS             complement(43016..43999)
                     /locus_tag="RH057_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3127064.1"
                     /translation="MNKRFVINMVSSLLLGAALISAPLQAAEKVVVNISKVDGMPWFN
                     RMGEGVVEAGKAFGVNASQVGPSSTDAPQQVKIIEDLIARKVNAITIVPNDANVLEPV
                     FKKARDAGIVVLTNESPGQPSANWDIEIIDNEKFAAEYVEHMAKRMGGKGGYVIYVGS
                     LTVPQHNLWADLLVKYQKEHYPDMHEVTRRMPVAESVDDSRRTTLDLMKTYPDLKAVV
                     SFGSNGPIGAGRAVKEKRAKNKVAVYGMMIPSQAASLIKSGDITEGITYDPASAGYAL
                     AAVASTLLKGEEIKPGLEMQNLGKADVDMDKRIIRFHKVLLVNKDNIDSLY"
     gene            complement(44253..44663)
                     /locus_tag="RH057_000649"
     CDS             complement(44253..44663)
                     /locus_tag="RH057_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV3127065.1"
                     /translation="MTIKEKFSQKYPHASFCTFGDSAALADHLATLIATGVKTAACGS
                     LAGCIEDNAFPMIGEYKIVENSRGEPVCVIRVIGLHLLRFSDVTVELARKEGEGDLSL
                     EYWRNEHRRFFQAEGSYSPEMDVIFEEYALIDVV"
     gene            complement(44962..46344)
                     /locus_tag="RH057_000650"
     CDS             complement(44962..46344)
                     /locus_tag="RH057_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HDV3127066.1"
                     /translation="MKESNSRREFLSQSGKMVTAAALFGTSVPLAHAAVAGTLNCEAN
                     NTMKITDPHYYLDNVLLETGFDYENGVAVQTRTARQTVEIQDGKIVALRENKQHPDAT
                     LPHYDAGGKLMLPTTRDMHIHLDKTFYGGPWRSLNRPAGTTIQDMIKLEQKMLPELQP
                     YTQERAEKLIDLLQSKGTTIARSHCNIEPVSGLKNLQNLQAVLARRQAGFECEIVAFP
                     QHGLLLSKSEPLMREAMQAGAHYVGGLDPTSVDGAMEKSLDTMFQIALDYDKGVDIHL
                     HETTPSGVAAINYMVETVEKTPQLKGKLTISHAFALATLNEQQVDALANRMAAQQISI
                     ASTVPIGTLHMPLKQLHDKGVKVMTGTDSVIDHWSPYGLGDMLEKANLYAQLYIRPNE
                     QNLSRSLFLATGDVLPLNEKGERVWPKAQDDASFVLVDASCSAEAVARISPRTATFHK
                     GQLVWGSVAG"
     gene            complement(46354..47304)
                     /locus_tag="RH057_000651"
     CDS             complement(46354..47304)
                     /locus_tag="RH057_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase family protein"
                     /protein_id="HDV3127067.1"
                     /translation="MKELVVVAIGGNSIIKDNASQSIEHQAEAVKAVADTVLEMLASD
                     YDIVLTHGNGPQVGLDLRRAEIAHEREGLPLTPLANCVADTQGGIGYLIQQALNNRLA
                     RHGEKKAVTVVTQVEVDKNDPGFAHPTKPIGAFFSESQRDKLQKANPDWCFVEDAGRG
                     YRRVVASPEPKRIVEAPAIKALIQQGFVVIGAGGGGIPVVRTEAGDYQSVDAVIDKDL
                     STALLAREIHADILVITTGVEKVCIHFGKPQQQALDRVDIATMTRYMQEGHFPPGSML
                     PKIIASLTFLEQGGKEVIITTPECLPAALRGETGTHIIKT"
     gene            complement(47447..48865)
                     /locus_tag="RH057_000652"
     CDS             complement(47447..48865)
                     /locus_tag="RH057_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV3127068.1"
                     /translation="MSQSLFSQPLNVINVGIAMFSDDLKKQHVEVTQLDWTPPGQGNM
                     QVVQALDNIADSPLADKIAAANQQALERIIQSHPVLIGFDQAINVVPGMTPKTILHAG
                     PPITWEKMCGAMKGAVTGALVFEGLAKDLDEATELAASGEITFSPCHEHDCVGSMAGV
                     TSASMFMHIVKNKTYGNIAYTNMSEQMAKILRMGANDQSVIDRLNWMRDVQGPMLRDA
                     MKIIGEIDLRLMLAQALHMGDECHNRNNAGTTLLIQALTPGIIQAGYSVEQQREVFEF
                     VASSDYFSGPTWMAMCKAAMDAAHGIEYSTVVTTMARNGVEFGLRVSGLPGQWFTGPA
                     QQVIGPMFAGYKPEDSGLDIGDSAITETYGIGGFAMATAPAIVALVGGTVEEAIDFSR
                     QMREITLGENPNVTIPLLGFMGVPSAIDITRVGSSGILPVINTAIAHKDAGVGMIGAG
                     IVHPPFACFEKAILGWCERYGV"
     gene            complement(48865..50412)
                     /gene="fdrA"
                     /locus_tag="RH057_000653"
     CDS             complement(48865..50412)
                     /gene="fdrA"
                     /locus_tag="RH057_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV3127069.1"
                     /translation="MSVKIVIKPNTYFDSVSLMSISTRANKLDGVEQAFVAMATEMNK
                     GVLKNLGLLTPELEQAKNGDLMIVINGKAGADNEQLLVEIEELFNSKAQSGSHEARYA
                     TIASAKKHIPESNLAVISVNGLFAAREARQALQNDLNVMLFSDNVSVEDELALKQLAH
                     EKGLLMMGPDCGTAIINGAALCFGNAVRRGNIGIVGASGTGSQELSVRIHEFGGGVSQ
                     LIGTGGRDLSEKIGGLMMLDAIGMLENDPQTEIIALISKPPAPAVARKVLERARACRK
                     PVVVCFLGRVETPVDEQGLQFARGSKEAALKAVMLSGVKQENLDLHTLNQPLIADVRA
                     RLQPQQKYIRGLFCGGTLCDETMFAVMEKHGDVYSNIQPDPEFRLQDINRSIKHTFLD
                     FGDDDFTNGKPHPMIDPTNRISRLIEEARDPEVAVIVMDFVLGFGSHEDPVGSTIEAI
                     KEAKAIAAAEGRELIILAYVLGTDLDTPSLEQQSQMLLDAGVILASSSTNTGLLAREF
                     ICKGEEA"
     gene            complement(50402..51265)
                     /locus_tag="RH057_000654"
     CDS             complement(50402..51265)
                     /locus_tag="RH057_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV3127070.1"
                     /translation="MWALTADADFLAQRGQGQVEQVFARAVNIALPARQQLLTLLCEE
                     YDNAPNSCRLALTHFDDLFRHGDKVQFDDQGITVGQHLHIEMSRCRRWLSPTLQMTAV
                     NFHLIAWLQWHDIIHQHLGENETLFNYRGDNPFYQALNKELHIKRRAVIQAVNEKQNI
                     AAAVASMIGLGIGLTPSADDYLTGLALILFIPGHPSEKYKEEFYLGMQRGRNNTTLLS
                     AITLEAALQQRCRENIHRFIHNIIYDIPGNATQAIEKIKHIGSSSGCDMLYGMADGCA
                     LSQTYGGNYVS"
     gene            complement(51305..51910)
                     /locus_tag="RH057_000655"
     CDS             complement(51305..51910)
                     /locus_tag="RH057_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ankyrin repeat domain-containing protein"
                     /protein_id="HDV3127071.1"
                     /translation="MSIKNLPADYLLAAQQGDIDKVKTCLALGVDINTCDRQGKTAIT
                     LASLYQQYACVQALIDAGADINKQDHTCLNPFLISCLNDDLTLLRIILPAKPDLNCVT
                     RFGGVGLTPACEKGHLSIVKELLAHTEINVNQTNHVGWTPLLEAIVLNDGGIKQQAIV
                     QLLLEHGASPHLTDKYGKTPLELARERGFEEIAQLLIAAGA"
     gene            52168..52665
                     /locus_tag="RH057_000656"
     CDS             52168..52665
                     /locus_tag="RH057_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV3127072.1"
                     /translation="MNGLTATGVTVGICAGLWQLVSSHVGLSQGWELLGTIGFVAFCS
                     FYAAGGGKSGFIRSLAVNYSGMVWAFFAALAAGWLASVSGLSAFWASVITTVPFSAVV
                     VWQGRFWLLSFIPGGFLGMTLFFASGMNWTVTLLGFLAGNCVGIISEYGGQKLSEATT
                     KRDGY"
     gene            52757..53689
                     /locus_tag="RH057_000657"
     CDS             52757..53689
                     /locus_tag="RH057_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3127073.1"
                     /translation="MNSIFTEENLLAFTTAARFGSFSKAAEELGLTTSAISYTIKRME
                     TGLDVVLFTRSTRSIELTESGRYFFRKATDLLNDFHAIKRSIDTISQGIEARVRICIN
                     QLLYTPKHTARLLQVLKKQFPTCQITVTTEVYNGVWDAIINNQANIAIGAPDTLLDGG
                     GIDYTEIGAIRWAFAIAPDHPLAFVPEPIAESQLRLYPNIMVEDTAHTINKKVGWLLH
                     GQESILVPDFNTKCQCQILGEGIGFLPDYMVREAMTQSLLVTRQIHNPRQDSRMLLAT
                     QHSATGQVTQWIKKQFAPNGILTGIYQDLLHREN"
     gene            complement(53731..54819)
                     /locus_tag="RH057_000658"
     CDS             complement(53731..54819)
                     /locus_tag="RH057_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3127074.1"
                     /translation="MASHDLSVFLEEFGATVNLTLPGIVSEKERLLLKLLMEGMSVTE
                     ISQYRNRSAKTISHQKKQLYEKLGIQSDITFWRDIFFQYHPQVISGTGNKNNFYIPDN
                     RCHHIVTPEAISLALENHEFKPWIQPVFCAQTGVLTGCEVLVRWEHPQTGIIPPDQFI
                     PLAESSGLIVIMTRQLMKQTADILMPVKHLLPDNFHIGINVSAGCFLAAGFEKECLNL
                     VKKLGNDKIKLVLELTERNPIPVTPEARAIFDSLHQHNITFALDDFGTGYATYRYLQA
                     FPVDFIKIDKSFVQMASVDEISGHIVDNIVELARKPGLSIVAEGVETQEQADLMIGKG
                     VHFLQGYLYSPPVPGNKFISEWVMKAGG"
     gene            complement(54961..58944)
                     /gene="ehaA"
                     /locus_tag="RH057_000659"
     CDS             complement(54961..58944)
                     /gene="ehaA"
                     /locus_tag="RH057_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaA"
                     /protein_id="HDV3127075.1"
                     /translation="MNKIYRLKWNRSRNCWSVCSELGSRVKGKKSRAVLISAISLYSS
                     LVFADDVIVNQDKTIDFGKENQSIDYRITVTDNANLVINATDTSRPRLTLASGGGLDI
                     TGGKVTINGPLNFLLKGTGFLNVSNAGSELYADDLYESNSGMRHDRGYFNVSNGGKIH
                     VKGTSRLTYLQGNVSGEGSQVNSKTFFMGVYGSYGGNQYLSVNNGGEVNAREHISLGY
                     YDQRSDTTLVVSDGGKISAPKISLSTNSELALGAQEGSAAKAAGIIDAEKIEFVWAKT
                     SDKKITLNHTDKNATISADIVSGSEGLGYINALNGTTYLTGDNSAFSGKVKIEQNGAL
                     GITQNIGTAEINNRGKLHLKADDSMTFANKISGNGTISIDSGTVALTGNNYAFSGYID
                     VASGAVVVISEDKNIGRADLDVDGKLQINANKDWVFDNDLQGRGIVEINMGNHEFSFD
                     EFAYTDWFQGSLAFQNTTFNLEKNAEFLQRGGITAGQGSQVIVGKGAHSISTLGFSGG
                     TVDFGALTAGAQMTEGTVNVSKTLDLRGEGVIQVSDSDVVSSVSRDIDSALSLTEVDD
                     GNSTIKLVDAQGAEVLGDAGNLQLQDKNGQILSSSAQRDIQQNGQKAAVGTYDYRLTS
                     GVNNDGLYIGYGLTQLDLHATDSDALVLSSNGKSENAADLSAKITGSGDLAFSSQKGQ
                     TVSLSNKDNDYTGVTDLRSGTLLLNNDNVLGNTHELRLAAETELDMNGHSQTVGTLNG
                     SADSLLSLNGGSLTVTNGGTSTGSLTGSGELNIQGGTLDIAGDNSNLTANVNIANSAN
                     VLVSHAQGLGSANVENNGTLALNNSAEKRAAASVNYTLGGNLTNNGTLMTGMSGQQAG
                     NVLVVKGNYHGNNGQLVMNTVLNSDDSVTDKLVVEGDTSGTTAVTVNNAGGTGAKTLN
                     GIELIHVDGKSEGEFVQAGRIVAGAYDYTLARGQGANSGNWYLTSGSDSPELQPEPDP
                     MPNPEPNPNPEPNPNPTPTPGPDLNVDNDLRPEAGSYIANLAAANTMFTTRLHERLGN
                     TYYTDMVTGEQKQTSMWMRHEGGHNKWRDGSGQLKTQSNRYVLQLGGDVAQWSQNGSD
                     SWHVGVMAGYGNSDSKTISSRTGYRAKASVNGYSTGLYATWYADDESRNGAYLDSWAQ
                     YSWFDNTVKGDDLQSESYKSKGFTASLEAGYKHKLAEFNGSQGTRNEWYVQPQAQVTW
                     MGVKADKHRESNGTLVHSNGDGNVQTRLGVKTWLKSHHKMDDGKSREFQPFVEVNWLH
                     NSKDFSTSMDGVSVTQDGARNIAEIKTGVEGQLNANLNVWGNVGVQVADRGYNDTSAM
                     VGIKWQF"
BASE COUNT        14132 a        16038 c        14744 g        14310 t
ORIGIN      
        1 tatgaattgc ataaaaacat tacccagtcg aacctccggg tctgaaagcc cttgcttttc
       61 agttattagc aacatttaac atttataaat taacatctgg ctgcgccaga gattacgtct
      121 ctgagtgcat cgttgtgctt gtagtataat aaataaaatg gagtgtcatt aagtggataa
      181 ggttttagat tcagccctcc tttcttcggc aaataaaaga aagggtatcc ttgctattgg
      241 cgcacatcct gacgatatag aattaggctg tggcgcatcg cttgctcgtc ttgcgcaaaa
      301 aggaatttat atcgcagccg tggtgatgac taccggcaac tctggtacag atggaataat
      361 cgatcgccat gaagaatcgc gcaacgccct aaagatatta gggtgccacc aaactattca
      421 tcttaatttt gctgacaccc gcgctcattt acagctcaat gatatgattt ccgctctgga
      481 agacatcatt aaaaatcaaa ttccttctga tgttgaaatc atgcgggtat ataccatgca
      541 tgatgccgac cgccatcagg atcatctcgc tgtttatcaa gcttcaatgg ttgcctgccg
      601 cactattcca caaattctcg gctacgaaac cccaagtacc tggctttcat ttatgcctca
      661 ggtttttgaa tccgttaaag aagaatattt cacagtcaaa cttgctgcat taaaaaaaca
      721 taaaagccag gaacgacgtg attatatgcg tcatgatcgc ctgcgtgcag ttgcacaatt
      781 tcgcgggcaa caggtcaata gcgatctggg tgaaggcttt gtaattcata aaatgattct
      841 ttgagcaaat tgcatgaaaa cctggatatt tatctgtatg tccatagcaa tgttgctatg
      901 gtttttaagt acgctaagac gtaaacccag tcaaaagaaa ggctgtattg acgccattat
      961 acctgcgtat aacgaaggcc cgtgtctggc gcagtcactg gataatctac tgcgtaaccc
     1021 ttatttttgc cgggtaattt gcgttaacga cggctccacg gacaataccg aagcggtcat
     1081 ggcggaagtc aaacgcaaat ggggcgaccg ctttgttgcc gtcacgcaaa aaaataccgg
     1141 taaaggtggt gcgctgatga atggcctcaa ttatgccacc tgcgaccagg tttttttaag
     1201 tgatgccgac acctatgttc cgcccgatca agacggaatg ggctatatgc tggcagaaat
     1261 tgagcgcggt gctgatgccg taggcggcat tccctctact gcgttgaaag gcgcggggct
     1321 gttaccgcac atccgcgcga ccgtaaagtt gccgatgatt gttatgaagc gcacgctaca
     1381 gcagctcctg ggcggcgcac cgtttattat cagcggtgcc tgcgggatgt tccgtactga
     1441 tgtattgcgt aagttcggtt tctcggatcg tactaaagtc gaagaccttg atctcacctg
     1501 gacattagtg gcaaacggct accgtattcg gcaggcgaat cgctgcatcg tatacccaca
     1561 ggaatgcaac agcccgcgtg aggagtggcg tcgctggcgg cgttggattg tgggatacgc
     1621 ggtctgtatg cgcctgcata aaagactttt atttagccgc ttcggtatct tcagtatatt
     1681 tcctatgctg ttggttgtga tttatggcgt tgggatttat ctcactacct ggtttaatga
     1741 attcatcacc accgggccgc atggtgtggt gttggcaatg tttccgctta tctggatcgg
     1801 cgtagtttgt gttattggtg cttttagcgc ctggtttcat cgttgctggt tgttggtgcc
     1861 tttagcgccg ctttccgttg tgtatgtatt attagcttat gccatctgga ttatttatgg
     1921 acttattgcc ttttttactg gacgcgaacc tcagcgcgac aaacccaccc gctattccgc
     1981 actggtggaa gcgtcaaccg cttattccca accttctgtc acaggaactg aaaaactatc
     2041 tgaagcttaa tgttaaagag aaaaatattc agattgcaga ccaggtaatt attgatgaaa
     2101 ctgcaggtga agttgttatc ggcgcgaata cccgtatttg tcatggtgcc gttattcagg
     2161 gtccggtagt gattggcgca aactgcctga taggtaatta tgcgtttatt cgtcctggca
     2221 caataatcag caatggtgta aaaattggtt ttgccaccga aattaaaaat gcggttattg
     2281 aagcggaagc aacgattggt ccgcaatgtt ttattgccga ctcggtagtt gcaaaccagg
     2341 catatttggg cgcacaagta cgtaccagta atcatcgtct ggatgaacag cctgtgtctg
     2401 ttcgaactcc agagggaatt atcgctaccg gatgcgataa attaggttgt tatatcgggc
     2461 agcgttcacg ccttggcgta caagttataa ttttgcctgg gcgaattatt tccccgaaca
     2521 cgcaacttgg cccgcgcgtg attgtagaac gtaatttacc tgccggaact tattcactcc
     2581 gacaagaact tatccgtaca ggagattaaa atgataaaac ggacgttatt agcggcggcc
     2641 atttttagcg cattgcccgc ttatgccggg ttaacttcca ttaccgcggg ctacgatttt
     2701 accgattatt ctggcgatca tggcaaccgt aatttagcgt atgctgaact ggtggcgaaa
     2761 gttgaaaacg caacgctgct ttttaatctt tcccaggggc gtcgtgatta tgaaactgaa
     2821 catttcaatg ccactcgcgg tcagggtgcg gtctggtata aatggaataa ctggctgaca
     2881 acccgaacgg gtattgcctt tgcggataat acgccggtct ttgcccgcca ggattttcgt
     2941 caggatatta acctggccct gttgccaaaa acgcttttca cgaccggtta tcgctacact
     3001 aaatattacg atgatgtcga agtcgatgcc tggcaaggcg gcgtatcact ctatactggc
     3061 ccggtgatca ccagctaccg ctatacccat tatgactcca gcgatgcagg tggtagttat
     3121 agcaatatga tttccgtgcg tctgaatgac ccgcgcggca ctggttatac gcaactatgg
     3181 ctaagccgcg gaacaggtgc ttacacctat gactggacgc cagaaacacg ctacggcagc
     3241 atgaagagcg tcagtctgca acgtattcaa ccgctgactg agcaacttaa tctcgggctg
     3301 acggcaggta aagtgtggta cgacacccca accgatgatt ataacggtct gcaacttgca
     3361 gcccatctga cctggaaatt ctgattcctt ctgccgcccg ctatccgggg cggccttccc
     3421 tgccgattag cccccccttt cctctttgtt ttccgaccac attcaccgga taaattttat
     3481 tctccagtgt tatatactat aggggggtat gcattgacat atagcatacc cccctatagt
     3541 atattgcgtg cagatgatga ggtgcgaaat gcccagtact ccggaagaga agaaaaaggt
     3601 ccttactcga gttcgtcgta ttcgagggca gattgatgct ctggaacggt cgctggaggg
     3661 tgatgccgaa tgccgtgcca tactccaaca gatcgctgcc gttcggggcg cggctaacgg
     3721 gctgatggca gaagtgcttg aaagccatat ccgggaaacg tttgaccgaa atgactgcta
     3781 cagccgcgaa gtcagccaat ccgttgacga cactattgaa ctggttcgag cctatcttaa
     3841 atagctgaat ctattaccat attgaggaag agcgagagat gaaatcacgt gctgccgttg
     3901 catttgctcc cggtaaaccg ctggaaatcg ttgaaattga cgttgcacca ccgaaaaaag
     3961 gtgaagtgct gattaaagtc acccataccg gcgtttgcca taccgacgca tttaccctct
     4021 ccggggatga cccggaaggt gtattcccgg tggttctcgg tcacgaaggg gccggcgttg
     4081 tggttgaagt cggtgaaggc gtaaccagcg tcaaacctgg cgaccatgtg atcccgcttt
     4141 acaccgcgga gtgcggcgag tgtgagttct gtcgttctgg caaaactaac ctctgtgttg
     4201 cggttcgcga aacccagggt aaaggcttga tgccagacgg caccacccgt ttttcttaca
     4261 acgggcagcc gctttatcac tacatggggt gctctacatt cagtgaatac accgtggtcg
     4321 cggaagtgtc tctggccaaa attaatccag aagcaaacca tgaacacgtc tgtctgctgg
     4381 gctgtggcgt gaccaccggt attggcgcgg tgcacaacac agctaaagtc cagccaggtg
     4441 attctgttgc cgtgtttggt cttggcgcga ttggtctggc agtggttcag ggcgcgcgtc
     4501 aggcgaaagc gggacggatt atcgctatcg ataccaaccc gaagaaattc gatctggcgc
     4561 gtcgcttcgg tgctaccgac tgcattaacc cgaatgacta cgacaaaccg attaaagatg
     4621 tcctgctgga tatcaacaaa tggggtatcg accatacctt tgaatgcatc ggtaacgtca
     4681 acgtgatgcg tgcggcgctg gaaagtgcgc accgcggctg gggtcagtcg gtgatcatcg
     4741 gggtggcagg ttctggtcag gaaatctcca cccgtccatt ccagttggtc actggtcgcg
     4801 tatggaaagg ttccgcgttt ggcggcgtga aaggtcgttc ccagttaccg ggtatggttg
     4861 aagatgcgat gaaaggtgat atcgatctgg aaccgtttgt cacgcatacc atgagccttg
     4921 atgaaattaa tgacgccttc gacctgatgc atgaaggcaa atccattcga accgtaattc
     4981 gttactgatt tcccgcaggt ataccccgtc cacttcagac ggggttctta atactctccc
     5041 tgggcagccg tccgggggat taaccgtgag ataatgactg atggaactca ttgaaaaaca
     5101 tgccagcttt ggcggctggc aaaatgtgta tcggcattat tcccaatcac tgaaatgtga
     5161 aatgaatgtc ggcgtctatc tcccaccaaa agccgcgaat gaaaaattgc cggtgctgta
     5221 ctggctttcg ggcctgacct gcaacgagca gaatttcatt actaaatcgg ggatgcagcg
     5281 ttacgcggct gaacacaaca ttattgttgt tgcgccggac accagtccgc gaggcagtca
     5341 tgtcgcagat gccgaccgtt acgatctcgg gcaaggtgcc gggttttacc tgaacgcaac
     5401 gcaagcgccg tggaatgaac attacaaaat gtatgactat atccgcaacg agctgccgga
     5461 tttagtgatg caacattttc cggcaacgac cagaaagtct atctctggtc attctatggg
     5521 cgggctgggc gcgctggtgc tggcgttacg taacccagat gaatatgtca gcgtctcggc
     5581 gttttcgccc attgtctccc catcgcaagt accgtgggga cagcaagcct ttgccgcata
     5641 tcttggtgaa aataaagatg cctggttgga ttacgatccg gtgagtctta tttcacaagg
     5701 tcaacgcgtt gcggaaatca tggttgatca ggggttgagt gatgattttt acgcggaaca
     5761 gctgcggact ccaaatcttg aaaagatctg ccaggagatg aatatcaaga cgttaatccg
     5821 ttatcacgag ggttacgacc acagctatta ttttgtctcc agttttattg gcgagcatat
     5881 tgcctaccac gccaataaac tgaatatgcg ttgataatag tgcacgactg ccggatgcgg
     5941 cgtgaacgcc ttatccggcc tacacttcgc ccgtaaaccg taggcctgat aagacgcgca
     6001 aagcgtcgca tcaggcatgg cgcacgactg ccggatgcgg cgtgaacgcc ttatccggcc
     6061 tacacttcgc ccataaaccg taggcctgat aagacgcgca tagcgtcgca tcaggcatct
     6121 gcgcacgact tatctatgtt taccacatca aatcatcagg cactttgaag tcggcatacg
     6181 gatcgtcttc atcctgctct tcggcactga gcgcgctgtg caacacaata ctgctggcat
     6241 cacgctgagc aattttatcg gcaacgctgg cgggaataat ggcgtattca ccttcgctat
     6301 tgttatcgac caacaagcgg gcaatcgcca gacgaccatt aatcaactgc gcctgtgtga
     6361 gcttatcgac aaaaatcttc ttaatcagat tgccgtcagt gaagttaaaa ccaatatcgc
     6421 cattggcaat ggtgattcgg ttcatttcaa tgagctgctt cacctgagct ttatattctt
     6481 tcgccaacgc cgcttgtttt tgctgttcgc taagctgttt atcacgctca agctgtgcct
     6541 ttttattttc ttctaccgcc gcccgagctt cacgcgcctg aacgcgcgat tttttcgccg
     6601 ttctctcgac cttcgccgct tttttgctgg tgactaatcc tgctttgagc aactgctctt
     6661 gtaaggtaag ttttgccatc tttgtttcca aacccgctga ataattttta gcgagtatac
     6721 ctgtaaacgc cgtgggtgta ttgggggcaa gtccctttcg cccctgctta ataaggcgcg
     6781 tcaggcatcg gcacacggct gcattcttga tctccgactc atcaaaataa acaccgccaa
     6841 cccagcaaca agaatacccg gtgcagaagc ggccattacg ccgaccgtgc cagtgcctaa
     6901 tgccagcatt ttcccggcca gtaacggacc gctcatagcc cccagacgcc ctaccgccac
     6961 ggctgttccc acacctgttg cgcggatctg cgaactgtaa aacaacggtg ccagggcata
     7021 caaaacgctt tgtccacctg tcgcaaacaa ccccgcgaca aatcccgcca gcaacatacc
     7081 gttaaatgac gacaccgttc caagcgccag cagcgaagct aacatgccgc tataaatcag
     7141 tagcgacatg gttactggac gcagcttatc catcaatgcg cccaacatta acgtcccgct
     7201 tgccgccccc atttgcaggg caaacatcac ccctgccgcc tgcgatggct ggaatccttg
     7261 ctccaccaaa agtagcggta gccagttgat caacatataa accaccagca gagtgaagaa
     7321 ataacacaac cacagcagca gcgtcgcggt tgccgtttct ggcgcaaata aggcacgcag
     7381 tggtggcgca gcctgttttt cgccagcgaa aaccgctgac tccggcagcc agcgcattaa
     7441 tagcggcacc agaatcaacg gcaccacacc acctacccaa aacaccgttt gccatgctaa
     7501 gtttgccccc gcgaaaccca gtgtcgccgc cagcgccgcg ccaatgggaa caccgcaata
     7561 catcaggctc actgctgtcc cacgaaaacg tggacccgcg gcttcagacg tcagggcgat
     7621 aagattcggc aacgccgccc ccagcccgac accggtcatc agccgcgcaa agaccagtga
     7681 ggggaaatcc caggcaatcg ccgttgccag cgagaacaaa ccaaacagcg caactgagcc
     7741 aatcaaaatg cgcttgcgac cataacggtc cgccagcatt ccgccaacca acgcgccggg
     7801 tagcaaaccg agtattccgg cgctaaatat ccagcccatt tgcattttat cgagtgcgaa
     7861 agcctgggcg atgccacccg ccgcaatgcc agccgcctga agatccagcc cttccatcag
     7921 agcgaccaaa aaacaaagcc cgatagtcag catcaggcgg gatgaagatg atgaaggggt
     7981 acgagtcgac ataagatgaa cctcgttgcg agtgcaggtg aatcgcgact gatacattgc
     8041 cagccgcgta atcagaacta tttaatgact gcctacgggc gaaatgtagg ccggacaaga
     8101 cgtttatcgc atccggcagt cgtgtgcaga tgcctgatgc gacgctaacg cgtcttatca
     8161 ggcctacaaa ttctcacgcc acccgtaggc cggataaggt gtttacgccg catccggcag
     8221 tcgtgctggg atgggcatac aaaatgcacg ttctcggcag tcatcattat ttgttgttgc
     8281 gcagatccag cgccacgtca acgatcatat cctcctggcc gccaaccatc cggcgtttgc
     8341 ccagctcaac gagaatatcc accgcactta agccataacg cgccgccgcc gtttcacagt
     8401 gacgcaggaa gctcgagtaa acaccagcgt atcccagcgc cagcgtttcg cgatcgactc
     8461 gtaccggtcg atcctgcaac ggacgcacca ggtcgtcagc ggcatccatt aacgcataga
     8521 gatcggtccc atgctgccag cccagtttat ccgcggcggc aataaatact tccagcggcg
     8581 cgttacctgc gcccgcgccc attcccgcga ggctggcgtc gattcggtcg cagccctctt
     8641 ccaccgccgc gatagagttc gccacgccaa gactcaggtt atggtgagcg tgcatgccag
     8701 tttgcgtttc tggtttcagc tctgctttca gggcgcggaa acggtcacgg atatcgctca
     8761 tgttcatcgc accgccagaa tccaccacat aaatacaggt cgcaccgtag ccttccatca
     8821 gctttgcctg ctttgcgaga ttctccggcg tggtcatatg gctcatcatc agaaaaccaa
     8881 cggtgtccat tccgagctcg cgggcatact gaatatgctg ggcggaaaca tcagcttcgg
     8941 tacagtgcgt tgccacacga accacccgcg cgccagcctg ccaggcattt ttcagatcgt
     9001 gaatagtgcc gattcctggc agcaacaacg tcgcgatttt ggcgtgcttc accacatccg
     9061 ccgctgcttc aatccattca aggtcgctat gtgcgccgaa accatagtta aagctggaac
     9121 cttgcaaacc gtcgccgtgg gccacttcaa tcgaatccac gcgggcatcg tccagtgctt
     9181 tggcaatctg gcgaacgttt tccagcgaat actgatgacg aatggcgtgc ataccgtcac
     9241 gcaatgtgac gtccgagata taaagttttt taccgttcat gccgcttctc ctgccttgcg
     9301 cgccagtgac tgggccattt tttccgctgt cgccagcgca ctggaagtca taatatcgag
     9361 gttgcccgca taggcaggca gataatgcgc tgcgccttcg acttccagcc agaccgctgt
     9421 tttcagtccg gagaattgcc ccacgcccgg taaattgacc ggtttatcct gcgggataac
     9481 ttcaaactgc acgcgctgtt tcaggcgata acccggtacg taagcctgca ccgcctcagc
     9541 catttcattg attgaggctt cgatatcatc ttgtgaagct tcgtcgctca atacatacac
     9601 cgtgtcacgc atcatcaacg gtggctctgc tgggttaagc acaataatcg ccttcccttt
     9661 tgccgcgccg cccaccactt caatggctcg ggaagtggtt tccgtaaatt catcgatatt
     9721 ggcacgcgtg ccaggtccgg cagatttact ggcgatagaa gcgataattt cggcgtaatg
     9781 aacacgcgcc acgcgtgaaa ctgccgccac cattggaatg gtggcctggc cgccgcaggt
     9841 gaccatgttg acgttcagtt gatcgacgtt cgcctcgagg ttaaccaccg gcacgcagta
     9901 agggccgatg gcagcaggcg tcaggtcaat taagcgaata tccggtttcg cttcgcgtaa
     9961 agcggcatcg tttttcacat gcgcgcccgc gctggtcgca tcaaatacaa tgtcgatatc
    10021 agcaaattca ggcatgttca tcagcccgat caccccttca tgggtggtgg cgacgcccat
    10081 acgtctggca cgcgccagac cgtcggactg aggatcaatg ccgaccatca ccgccatctc
    10141 cagatgctga ccgtgacgca aaattttaat catcagatcg gtgccaatgt tgccagaacc
    10201 gataatggcg actttacgct tactcatgac agacttcctt ttggggctgc gcttgaaaat
    10261 gtcgcagcaa ctgaacctat gccttcaata tgggcttcaa aacgatcgcc cgcattcact
    10321 gccaccatcg gacctaatgc cccggtaaga atgatatctc cggcgcgcag cggttcaccc
    10381 aggctggcca ttttgcgtgc cagccagacg gcggcattaa gcggatgtcc caggcattcg
    10441 ctgccgcgcc cgctggaaac ctcttcgtta ttacgcgtca tcttcatggc gcagtttttc
    10501 aggtctaacc ccgccggacg ttgcgccgga ccgccgatga catacacccc gcaggaagcg
    10561 ttatctgcca cggtatcgac aaactgaatc gaccagtcgc gaatgcggct ccccaccact
    10621 tccagcgccg gaagtaccca ttcaatggcg ttatacagtt cgtcgaaggt gatatcagtt
    10681 gcgggtaaat cgcggttcaa caccagtgcg atctccgctt caatgcgggg ttgcagaaca
    10741 cgggaaaaag gaatgatttc gttatcgcca taacacatgt cggcaaataa cgtcccaaaa
    10801 tccggttgat caacgcccag ttgttgttgc actttcggat gcgtcaggcc cactttacgc
    10861 cctaccacgc gacgcccctg cacaacgtca tattgcacat ttatgtgctg aatggcgtag
    10921 gccgcttcag cgttatcgat accaatcaga tcgcgcagcg gcgcaatcgc ttcgccctgc
    10981 tctgcggcgc ggcgtaaatc cgccgccagt tgctcaagag tatgcttcgt catgaccatt
    11041 ccttaagggc gtgcgaggaa attcagcacc agttgattga aagcgtcggc atgttcccac
    11101 tgcgcccagt gaccacagtc gcggaagata tgcagttcag aaccggcaat gccggacagc
    11161 agacgcagac ccgcatccat cggcacaaag cggtcgttgc gcccccagac aatcagggtt
    11221 tgcgctttga tttccgccag acgtgggcca aaatccggga actgtttcgg attagcttcc
    11281 aggctcttaa cgaagttttc caggtgatcg cggcgcgaca gcatattatt caggcgcgct
    11341 tcaaacaggg cgtcggtcaa atcgctggta tcgaaaacga agatatccat catcagcttc
    11401 aggttttcga tagtcggctg acgataaagc tgattcagtc gcttaatacc ttcggttggc
    11461 atcggcgtaa acaaactcat gccgcccgta ccgccgccca tcagcaccag tttgccgacg
    11521 cgctccggcc agttaagggt gaacgccaca gaactatggc cgcccatcga gttgcccagc
    11581 aggtggattt tggcgatatc cagttgatcc accacgcttt tcaggattcg tgcattaaga
    11641 tccgatcgtg aaccactatt aacgatcgaa tcgcttttgc cccaacccgg acaatccagc
    11701 aggattaccc gatagcccgc ctctaccagc ggatcgatat tgcggctgaa gttcgcccag
    11761 ccagtagcac ccgggccgga accatgcagc aggacaacgg tttcgtcgcc ttgtccgcag
    11821 tcattaaaat ggatgcgcag cgttttaccc gcttcttcta cattcagaaa acggctggtg
    11881 gcggcttcgg tttgtggctg ataactcatc atcttctcct gcatattcag ttctctgttc
    11941 tggcgcttaa cgagccaaat ccggcaatcc actccgggat tgggcggtaa taacgccctt
    12001 cgctacgcca gttgccaaat gtagaaatag cggcaaaagc ggcgacccag gttttgattt
    12061 catgtgtcga ctttccggca atggcggaaa gctcttcgtt actgacggca tccagttcct
    12121 gtatgcgtcc ctgctccagc aaagtcatga actggttatc ccaaatcggg ttgagcggat
    12181 gcagagttct ctgatcctca acaaacttct cagcggcgct aatcacccgt tgctgacgca
    12241 attcgcgctc actggcgggt aaatctttcc cgctccccaa cagacggtcg cgcatatggg
    12301 catcggcttt cgccagttcg ggcaccggcg gctgatggga aagcccaccg gaacccagga
    12361 acagcacgcg tttattgaga gtgctggtga aacgtccaat ggcttcaccc aacatgcggg
    12421 tacgctggaa accgggcagc ggcgtggcga caccgttgat gaacacaggc agaactggca
    12481 ccttatccag cccaccgagc aggaactcca gcggctgggc gaacccgtgg tccacctgca
    12541 tacagtaaga aacggcaaga tcgatcccgc tcttcatgac agcatgcgca caggcctccg
    12601 ccagctccac aggcacgggc agctctcctg ccgcactgcc gaaatcacca attgccgtcg
    12661 ctccaacgcc taaacagaac ggtggcatca cgtcatagaa aaagccgttg tagtgatctg
    12721 gcgcaaacag caccaccagt tcaggggaga atgccgcaat acgctcgcgg gcgctggcaa
    12781 tcacgccatt gacctcatcg agcacctctt gcgccgggtc gacatacccc accagcggcg
    12841 agtgggaaag acagtgaaga taagcgtgca tatcaggcta ccttttcgac agaaacgtcg
    12901 gcatcagggc gagtcagcgt catcaccgac gccagtttat tcagggtctt gcccagggtt
    12961 tgcggaatgg cggtggcggc aacaaagcga tccgggcgca tcaccaccag cgaagcattg
    13021 tgttgcgcga accagctacg caggcgacct tgcgtatcgc ccacgcgtag tacgccgtcg
    13081 tggttatcct gtgcggtatg aatttgcact tccggcacca cctgaatgaa gcgtgtgccc
    13141 aacgcgcgcc actgctggat ttgctcgtcg ctcatccccc acagtggatt gcatccccag
    13201 ccaattaccg cgaagttcgc gccgatcgcg ttatcgagca gcgtcacgtc gccgttttcc
    13261 agcgtgactt tcggctgaat aaacatcttg ccgaccggag agtgcttcgc ttcgccctca
    13321 cgcatcagcg caccgccgta atattgcggc atcggcttga agcgcatttc gaggaagtag
    13381 cgttttactg gcggcagata attcaacagc caggaaacgc cgtcacgtaa cgtaccctgc
    13441 cagcgtttcg gcggagccag cacgttgccc gccgtcacgg acaggtcaat catcgctttg
    13501 gcgtgatcgc gacgttcttg ttgataggta tcgagcagcg catcgcgggc tttcccctgg
    13561 ataaccaacg ccagtttcca tgcgaggtta aaggcgtcgc gcataccact gttatagccc
    13621 tgcccctgcc ataccggcat gatgtgcgcg gcatcgcccg ccagcagtac gcgatcaata
    13681 cggaaacgtt gcgccagtcg cgcgttgtgg gtgtagacac gctggcgaat caattcaaca
    13741 ttgtccggat taggcagcac tttgcttaac agcttgcgca tattttgcgg ctcacgcagc
    13801 tgctcttcgg tttctcccgg catcaccata aattcaaagc gacgtaccgc atgaggcagc
    13861 gcggcagaaa catacgggcg caccggatcg caacacaaat agatatgcgg cgtacttaac
    13921 ggatcgttgg cgatatctac cacaatccac tgatttggcg cagttttacc ttcaaacggc
    13981 acattcagag tgcgacggac aaaacttgct ccaccgtcac aggctaccag ccactgggct
    14041 ttgaccgttt cccgctgccc ttctgccgtt ttcaggtgca aggtcacttc gtcattgtgc
    14101 tggctgaagg cctccagctc gcgggcaaac aagcagcgca cattcggaaa acgcgacaac
    14161 ccttccagca tcaccgcatc gacctgtggc tgaataaagg cgttacggcg cggccagcca
    14221 aattcatcgg tcattggctg aatatcagca aaacagcggc cttttggggt gagaaaacgc
    14281 atcgcgtgcc acggtgtagt gtgcggcaga acattctcga ccaggccgac cgactgcatg
    14341 gtgcgcagcg cctcgtcatc aataccaatc gcacgcgggt agtcgatcaa cttatcgagt
    14401 ttctccacca ccagcacgtc aatgcccatc tgaccgagat agttcgccat catcagccca
    14461 accggaccgg caccagcgat cgccacctga acgctatggt taacagcagg ctggatgtca
    14521 gggtgttgta ttgccatttc agtacctcac gactcggaca aaatgccgtt gcgcgcacag
    14581 tacagcgcaa cttattttgt taaaaacatg taaatgattt tttattgtgc gctcagtata
    14641 ggaagggtat tttcggctac aatcaaaaca tgcccgaatg tgcaccaggt gcacaacgtt
    14701 gttttaacta tagaaatgtc aattaatatg cagaacaatg agcagacgga atacaaaacc
    14761 gtgcgcggct taacacgcgg tctaatgtta ttaaatatgt taaataaact tgatggcggt
    14821 gccagcgtcg ggctgctggc ggaactcagc ggcctgcatc gcaccactgt gcggcgactg
    14881 ctggagacgc tgcaggaaga gggatatgtc cgccgtagcc cctccgatga cagttttcgt
    14941 ctgaccatca aagtgcggca attaagcgaa ggatttcgtg acgaacagtg gatttctgca
    15001 ctggcggccc cgctgctggg cgatctgttg cgcgaagtgg tatggccgac agatgtgtcc
    15061 acgctggatg ttgatgcaat ggtggtacgc gaaaccaccc accgtttcag ccgcttatcc
    15121 tttcaccggg caatggtcgg gcgacgttta ccgctcctga aaaccgcctc gggcctgacc
    15181 tggctggcct tttgcccgga acaagaacgc aaggaattaa tcgaaatgtt agccgcccgc
    15241 cccggtgatg actatcaact ggcacgagaa ccgttaaagc tgcaagccat tctggcgcgc
    15301 gcgcgcaaag agggttacgg acagaactac cgcggctggg atcaggagga gaagatcgcc
    15361 tctatcgccg taccgctgcg cagtgaacaa cgggtgattg gctgtctgaa tctggtgtat
    15421 atggcgagcg caatgaccat tgaacaggca gcggaaaagc atcttccggc gctacaacgg
    15481 gtagcaaaac agatcgaaga aggggttgaa tcgcaggcta ttctggtggc cggacggcga
    15541 agcggcgtgc atttacgttg acaccatcga atggcgcaaa acctttcgtg gtatggcatg
    15601 atagcgcccg gaagagagtc aattcagggt ggtgaatgtg aaaccagtaa cgctatacga
    15661 tgtcgcagag tatgccggtg tctcttatca gaccgtttct cgcgtggtga accaggccag
    15721 ccacgtttct gcgaaaacgc gagaaaaagt ggaagcggcg atggcggagc tgaattacat
    15781 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
    15841 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
    15901 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
    15961 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
    16021 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
    16081 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
    16141 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
    16201 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
    16261 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
    16321 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
    16381 gatggcgctg ggcgcaatgc gcgccattac cgagtccgga ctgcgcgttg gtgcggatat
    16441 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
    16501 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
    16561 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
    16621 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    16681 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    16741 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    16801 gtgaaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacggat
    16861 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
    16921 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
    16981 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gctttgcctg gtttccggca
    17041 ccagaagcgg tgccggaaag ctggctggag tgcgatcttc ctgaggccga tactgtcgtc
    17101 gtcccctcaa actggcagat gcacggttac gatgcgccca tctacaccaa cgtgacctat
    17161 cccattacgg tcaatccgcc gtttgttccc acggagaatc cgacgggttg ttactcgctc
    17221 acatttaatg ttgatgaaag ctggctacag gaaggccaga cgcgaattat ttttgatggc
    17281 gttaactcgg cgtttcatct gtggtgcaac ggtcgctggg tcggttacgg tcaggacagt
    17341 cgtttgccgt ctgaatttga cctgagcgca tttttacgcg ccggagaaaa ccgcctcgcg
    17401 gtgatggtgc tgcgctggag tgacggcagt tatctggaag atcaggatat gtggcggatg
    17461 agcggcattt tccgtgacgt ctcgttgctg cataaaccga ctacacaaat cagcgatttc
    17521 catgttgcca ctcgctttaa tgatgatttc agccgcgctg tactggaggc tgaagttcag
    17581 atgtgcggcg agttgcgtga ctacctacgg gtaacagttt ctttatggca gggtgaaacg
    17641 caggtcgcca gcggcaccgc gcctttcggc ggtgaaatta tcgatgagcg tggtagttat
    17701 gccgatcgcg tcacactacg tctgaacgtc gaaaacccga aactgtggag cgccgaaatc
    17761 ccgaatctct atcgtgcggt ggttgaactg cacaccgccg acggcacgct gattgaagca
    17821 gaagcctgcg atgtcggttt ccgcgaggtg cggattgaaa atggtctgct gctgctgaac
    17881 ggcaagccgt tgctgattcg aggcgttaac cgtcacgagc atcatcctct gcatggtcag
    17941 gtcatggatg agcagacgat ggtgcaggat atcctgctga tgaagcagaa caactttaac
    18001 gccgtgcgct gttcgcatta tccgaaccat ccgctgtggt acacgctgtg cgaccgctac
    18061 ggcctgtatg tggtggatga agccaatatt gaaacccacg gcatggtgcc aatgaatcgt
    18121 ctgaccgatg atccgcgctg gctaccggcg atgagcgaac gcgtaacgcg aatggtgcag
    18181 cgcgatcgta atcacccgag tgtgatcatc tggtcgctgg ggaatgaatc aggccacggc
    18241 gctaatcacg acgcgctgta tcgctggatc aaatctgtcg atccttcccg cccggtgcag
    18301 tatgaaggcg gcggagccga caccacggcc accgatatta tttgcccgat gtacgcgcgc
    18361 gtggatgaag accagccctt cccggctgtg ccgaaatggt ccatcaaaaa atggctttcg
    18421 ctacctggag agacgcgccc gctgatcctt tgcgaatacg cccacgcgat gggtaacagt
    18481 cttggcggct tcgctaaata ctggcaggcg tttcgtcagt acccccgttt acagggcggc
    18541 ttcgtctggg actgggtgga tcagtcgctg attaaatatg atgaaaacgg caacccgtgg
    18601 tcggcttacg gcggtgattt tggcgatacg ccgaacgatc gccagttctg tatgaacggt
    18661 ctggtctttg ccgaccgcac gccgcatcca gcgctgacgg aagcaaaaca ccagcagcag
    18721 tttttccagt tccgtttatc cgggcaaacc atcgaagtga ccagcgaata cctgttccgt
    18781 catagcgata acgagctcct gcactggatg gtggcgctgg atggtaagcc gctggcaagc
    18841 ggtgaagtgc ctctggatgt cgctccacaa ggtaaacagt tgattgaact gcctgaacta
    18901 ccgcagccag agagcgccgg gcaactctgg ctcacagtac gcgtagtgca accgaacgcg
    18961 accgcatggt cagaagccgg gcacatcagc gcctggcagc agtggcgtct ggcggaaaac
    19021 ctcagtgtga cgctcccctc cgcgtcccat atcatcccgc aactaaccac cagcgaaacg
    19081 gatttttgca tcgagctggg taataagcgt tggcaattta accgtcagtc aggccttctt
    19141 tcacagatgt ggattggcga taaaaaacaa ctgctgacgc cgctgcgcga tcagttcacc
    19201 cgcgcaccgc tggataacga cattggcgta agtgaagcga cccgtattga ccctaacgcc
    19261 tgggtcgaac gctggaaggc gacgggccat taccaggccg aagcggcgtt gttgcagtgc
    19321 acggcagata cacttgccga cgcggtgctg attacgaccg ctcacgcgtg gcagcatcag
    19381 ggaaaaacct tatttatcag ccggaaaacc taccggattg atggtagtgg tcaaatggcg
    19441 attaccgttg atgttgaagt agcgagcgat acaccgcatc cggctcggat tggcctgacc
    19501 tgccagctgg cgcaggtcgc agaacgggta aactggctcg gattagggcc gcaagaaaac
    19561 tatcccgacc gccttactgc cgcctgtttt gaccgctggg atctgccatt gtcagacatg
    19621 tataccccgt acgtcttccc gagcgaaaac ggtctgcgct gcgggacgcg cgaattgaat
    19681 tatggcccac accagtggcg cggcgacttc cagttcaata tcagtcgcta cagccaacaa
    19741 caactgatgg aaaccagcca tcgccatctg ctgcacgcgg aagaaggcac atggctgaat
    19801 atcgacggtt tccatatggg gattggtggc gacgactcct ggagcccgtc agtatcggcg
    19861 gaattccagc tgagcgccgg tcgctaccat taccagttgg tctggtgtca aaaataataa
    19921 taaccgggca ggccatgtct gcccgtattt cgcgtaagga aatccattat gtactattta
    19981 aaaaacacaa acttttggat gttcggttta ttctttttct tttacttttt tatcatggga
    20041 gcctacttcc cgtttttccc gatttggcta catgacatca accatatcag caaaagtgat
    20101 acgggtatta tttttgccgc tatttctctg ttctcgctat tattccaacc gctgtttggt
    20161 ctgctttctg acaaactcgg gctgcgcaaa tacctgctgt ggattattac cggcatgtta
    20221 gtgatgtttg cgccgttctt tatttttatc ttcgggccac tgttacaata caacatttta
    20281 gtaggatcga ttgttggtgg tatttatcta ggcttttgtt ttaacgccgg tgcgccagca
    20341 gtagaggcat ttattgagaa agtcagccgt cgcagtaatt tcgaatttgg tcgcgcgcgg
    20401 atgtttggct gtgttggctg ggcgctgtgt gcctcgattg tcggcatcat gttcaccatc
    20461 aataatcagt ttgttttctg gctgggttct ggctgtgcac tcatcctcgc cgttttactc
    20521 tttttcgcca aaacggatgc gccctcttcc gccacggttg ccaatgcggt aggtgccaac
    20581 cattcggcat ttagccttaa gctggcgctg gaactgttca gacagccaaa actgtggttt
    20641 ttgtcactgt atgttattgg cgtttcctgc acctacgatg tttttgacca acagtttgct
    20701 aatttcttta cttcgttctt tgctaccggt gaacagggta cgcgggtatt tggctacgta
    20761 acgacaatgg gcgaattact taacgcctcg attatgttct ttgcgccact gatcattaat
    20821 cgcatcggtg ggaaaaacgc cctgctgctg gctggcacta ttatgtctgt acgtattatt
    20881 ggctcatcgt tcgccacctc agcgctggaa gtggttattc tgaaaacgct gcatatgttt
    20941 gaagtaccgt tcctgctggt gggctgcttt aaatatatta ccagccagtt tgaagtgcgt
    21001 ttttcagcga cgatttatct ggtctgtttc tgcttcttta agcaactggc gatgattttt
    21061 atgtctgtac tggcgggcaa tatgtatgaa agcatcggtt tccagggcgc ttatctggtg
    21121 ctgggtctgg tggcgctggg cttcacctta atttccgtgt tcacgcttag cggccccggc
    21181 ccgctttctc tactgcgtcg tcaggtgaat gaagtcgctt aagcaatcaa tgtcggatgc
    21241 ggcgcgagcg ccttatccga ccaacatatc aggacggaat gatcgcaatg aacatgtcga
    21301 tgacagaaag aataaaagca ggcaagctat ttaccgatat gtgcgaaggc ttaccggaaa
    21361 aaagacttcg tgggaaaacg ttaatgtatg agtttaatca ctcgcatcca tcagaagttg
    21421 aaaaaagaga aagcctgatt aaagaaatgt ttgccacggt aggggaaaac gcctggatag
    21481 aaccacctgt ctatttctct tacggttcca acatccatat aggccgcaat ttttatgcaa
    21541 atttcaattt aaccattgtc gacgactaca cggtaacgat cggtgataac gtattgattg
    21601 cacccaatgt tactctttcc gttacgggac accctgtaca ccatgaattg agaaaaaacg
    21661 gtgagatgta ctcttttccg ataacgattg gcaataacgt ctggatcgga agtcatgtgg
    21721 ttattaatcc aggcgtcacc atcggggata attctgttat tggcgcgggt agtgtcgtca
    21781 taaaagacat tccaccaaac gtcgtggctg ctggcgttcc ttgccgggtt attcgcgaaa
    21841 taaacgaccg ggataagcaa tattatttca gagattacaa agttgaatcg tcagtttaaa
    21901 ttataaaaat tgcctgatac gcttcgctta tcaggcctac aaattcagtg atctacgtag
    21961 gccgcatccg gcgtgaacaa agcgcagaaa caagagtcgc atcatgcctc tttgacccac
    22021 agctgcggaa aacgtgctgg tgcaaaacgc agggttataa tcatcagccc aacgacacac
    22081 agcgcatgaa acgcccagtc catcaggtaa ttgccgctga tactacgcag cacaccagaa
    22141 aaccaagggg caagcccggc gatgataaaa ccgatcccct gcataaacgc caccagcttg
    22201 ccagcaatag ctggttgcgc agagtgatcg agcgccagca gcaaacagag cggaaacgcg
    22261 ccgcccagac ctaacccaca caccatcgcc cacaataccg gcaattgcag cggcagccag
    22321 ataaagccgc agaaccccac cagttgtaac accagcgcca gcattaacag tttgcgccga
    22381 tcctgatggc gagccatagc aggcatcagc aaagctcctg cggcttgccc aagcgtcatc
    22441 aatgccagta aggaaccgct gtactgcgcg ctggcaccaa tctcaatata gaaagcgggt
    22501 aaccaggcaa tcaggctggc gtaaccgccg ttaatcagac cgaagtaaac acccagcgtc
    22561 cacgcgcggg gagtgaatac cacgcgaacc ggagtggttg ttgtcttgtg ggaagaggcg
    22621 acctcgcggg cgctttgcca ccaccaggca aagagcgcaa caacggcagg cagcgcccac
    22681 caggcgagtg tttgatacca ggtttcgcta tgttgaacta accagggcgt tatggcggca
    22741 ccaagtccac cgccgcccat cagagccgca gaccacagcc ccatcaccag tggcgtgcgt
    22801 tgctgaaacc gccgtttaat cactgaaggc atcaccgcct gaatgatacc gatccccacc
    22861 ccaccaagca gtgcgctgct aagcagcagc acactttggg ggtaaagctc acgcatcaat
    22921 gcaccgacgg caatcagcaa cagactgatg gcgacgctgc gacgttcgct gacatgctga
    22981 tgaagccagc ttccggccag cgccagcccg cccatggtga ccaccggcag agcggtcaac
    23041 agggcagcca cgctaaagct cattccgctc gcctggcgca attgcggtag cagtggaccg
    23101 acggaggtga gcagtggtcg catattaaga ccaatcagca ccagaaccag cagcatcgtg
    23161 aacgtctttt gatggttgcg catggctgtc agaacggttt ggtcggcaga tatttaccat
    23221 ctaaggtgat gaccgcacgt tcgccacctt ccgggtccgc cactttctta acgtcgagtt
    23281 tgaagttaat cgcgctgata atgccatcgc caaacttctc atgaaccaac gctttcaggg
    23341 ttgtgccgta cacctgcaac atttcataga aacgatacat cgttgggtcg gttggaatac
    23401 ggtcatcaat gcagccacgc agtggaatca tctgcaacag tagaatggcg tcttcgtcga
    23461 gatccagctt cgccccgacc aggcgggcgg cgtcggcagg aagcgcctgc tgccccagca
    23521 aagccgcggt tacaaaggct tctgccagac cggtgccgtc ggcaatctcg gcaaatgaga
    23581 gatctttttt agctttgctg agcaaaatgg catcggcaag atcaagacga atattgcggt
    23641 taatttgcga ctgaatcatg gtgcatctcc tgatggttta aaaataaggt tacgctgcgg
    23701 tcggttggcg tagcggtatg gcacaaacgc gcggattagc tgccagtggc acaaactggc
    23761 gggttgcgcc gtcaaacgct gcgatgctgc cgctttcaat gtcgtagacc cagccgtgca
    23821 gggcgatccg cccctcttcg agcgccaggc gcaccgatgg atgagtttgc aaattagcca
    23881 actgagcaat gacgttttca cgtaccatcg ccgcagcttt tgacggtaaa tcggaatgcg
    23941 ggcgcgcctc attaacgacg cgggctgaat cggcataacg cagccagtgg gagacggcag
    24001 gcatatggtc catgcactga cagctggcaa tggcggtcat cgcgccacag ttggaatgac
    24061 cacaaatcac aatgtcagat acccgaagcg cagcgacggc atactccacc gaagcagaaa
    24121 cgccaccggg ttccggcccg taggaaggga cgatattgcc cgcgttgcga ataacgaaca
    24181 gatcgccagg ctcacgttgc gtcaccagct cagggaccag acggctgtcg gagcaggaga
    24241 taaaaagtgt gcgcgggctt tgctgtgtcg ccagctgttt aaacaaggct tcccgcttcg
    24301 gaaatgcctc gcgctggaat ttaaggaatc catcaataat ctctttcacc gttaacctct
    24361 gtctgtctct ggaatgagag gcagactact cgccgattgt cataaggtaa aagtctcatt
    24421 tatgatgagt tctattggat ttacttatag gttggcgaat gctctctcga catatcaatt
    24481 actttcttgc cgtggctgaa catggcagct tcacccgtgc cgccagtgcg ttgcacgtct
    24541 cccagcctgc gctttcccag cagattcgcc agttagagga gagtttaggc gtgccgctgt
    24601 ttgaccgtag cgggcgaacg attcgtctca ctgatgcagg agaagtctgg cgacagtacg
    24661 ccagccgggc gttacaggaa ctgggggcgg gtaaacgggc gattcatgat gttgccgatc
    24721 tgacgcgagg atcgctgcgt atcgccgtca ctcccacctt tacaagctac tttatcggcc
    24781 ctttaatggc ggatttctat gcgcgctacc ccggcatcac gctccagcta caggaaatgt
    24841 cgcaggagaa aatcgaagat ctgctttgtc gcgacgagct ggacgttggg attgccttcg
    24901 cgcctgtgca ttcgccggag ctggaggcaa ttcctttact gacagaaagt ttagcgttag
    24961 tcgtggcgca acatcatccg ctggctgcct gtgaacaggt ggcgttgagt cgcttgcatg
    25021 atgaaaaact ggtcctgctc agcgcggaat ttgccaccag agagcaaatt gaccactact
    25081 gcgagaaagc ggggctacat ccacaggtgg tcattgaggc gaactcaatt agcgcggttc
    25141 tggagctgat tcgccgcact tccctttcca cattgttacc agcagcgatt gccacacaac
    25201 atgacgggct taaagcgatt tctcttgccc cgccactact ggagagaacg gcggttttgt
    25261 tgcggcggaa aaatagctgg cagacagccg ccgcgaaggc atttttgcac atggcgttgg
    25321 aggaatgtgc ggatgttggc gagaatgaat cacggtagcc actgacacag gtcagtggct
    25381 cttcaacaca gaggaaatta ccgtttgtaa tcgatggctt ctggctgctc cagatatacg
    25441 gtggtttgtg ccggttgtgt actggcaatc accttgccgc cacgtaccga ataacgtacc
    25501 ggaacctgac ggcgcagcgc atcaaatcca ttttcagccg gcaggataat caggtttgcg
    25561 ctgtttccgg cggcaatgcc gtaatcctgc aaattcaatg ttctggcact gtggtgggtg
    25621 attaaattca ggccatcgtt aatctgcccg tagcccatca actggcaaac atgcagcccc
    25681 atatgcagca cttgcagcat attcgccgtt cccagcggat accacggatc gaagacatca
    25741 tcgtgaccaa agcagacgtt aatgccggac tccagcatct ctttaacgcg cgtgatgccg
    25801 cgacgttttg gatacgtatc gaaacgtcct tgcagatgaa tattgaccag cgggttggcg
    25861 acaaagttaa taccggacat tttcagcaag cggaacaggc gtgaggtata cgccccgtta
    25921 taggagtgca ttgccgtggt gtggctggcg gtgactcgcg cgcccatgcc ttcacggtgc
    25981 gccagggcag caacggtttc gacaaagcgc gactgctcgt catcgatctc atcacagtga
    26041 acgtcgatga gacggtcgta tttttgcgcc agggcgaagg ttttatgcag cgactccacg
    26101 ccgtattcac gggtaaattc aaaatgcgga atcgccccca ctacatctgc ccctaagcgt
    26161 aacgcctctt ccagcaacgc ttcaccgttg ggatacgaca aaatcccttc ctgagggaag
    26221 gcgacaattt gcagatcaat ccacggcgcg acttcctgct tcacttccag cattgctttc
    26281 agcgcagtta gcgttgcatc cgaaacatcg acatgggtac gcacatgctg aatgccgttg
    26341 gcaatctgcc atttcagcgt ttgccaggcg cgttgtttca catcgtcatg ggttaataac
    26401 gctttgcgct cggcccagcg ttcaatgcct tcaaacagcg tgccggactg attccagttc
    26461 ggttgtccgg cggtttgcgt ggtgtccagg tgaatatgcg gctccacaaa cggcggtata
    26521 actaaacctt gttcggcatc caggctgttt tcagttatgg gcatcacgcc ggattgcgca
    26581 tcaatggcgc tgatttttcc gtcctgcaga tgaatctgcc acagcccctc tttgcctggt
    26641 aaccgggcgt taataattgt ttgtaaagcg ttattcgaca ctgttaacct ccacatgcgt
    26701 cattgctgct gtcgttttac gattcaaaat cgggttaagg atcagatagc tcagcgcgcc
    26761 acctaatacc gcgttgaccg gaacaattcc cggtaaccag tggcctgcgg caatccccag
    26821 cgcgaccgcc agaatcgcca cccaattgac actcatcata cgcgtggtcg caaagtgctc
    26881 atagcggcga cggttcatca gatagtcggc gatgatcacg ccccctaccg gaggaatagc
    26941 tgccgaaagg aaggtcaacc agccgacaaa attgttatac agccataatg cgcagaccgt
    27001 accgataata ccgttgatta ccgaaagggt tttgctcgac ataccggtaa tgttggcgaa
    27061 acctaaaccc gacgcataga gtgcgttatc gttggtggtc cagatattca gccccagcac
    27121 cacaatcgca ggcagcagca ggccctgagc aatcatcaca tcagagatat ccgccatgcc
    27181 cagtgccgca gcgcccgctg caccgaaaat aaacatcaac gagttgccga ggaaaaaggc
    27241 caccatcgcc accagcaccg ccagtttggc attgcgacca aaccggacaa agtcagcggt
    27301 gagcgtaccc gcactgataa atgaccccac aaccagcgcc agcgcgacat tgaaatctaa
    27361 cggttgtgcg ggaacgaccg cttttaatgc gtccaggccg cccatgccgt taacagccag
    27421 ccacacggaa taaccgccca ggcaggcgat agccggaacc gcaatcaccg aaagaaccgt
    27481 cagcgccgaa atgccaaaaa agacggtgac ggtcatcagt aaaccggaaa cggcaatcag
    27541 caaattaata tccagcccgg ttgccttacc caccggaatg gcaaacatcg ccacaccgac
    27601 gccaaaccag ccaacctgag ttccgcccag tagcagtgaa ggcagccatg agcctttaac
    27661 accaaacgag aagcgagcaa gaagatgagt ggtcaggccg gtttttgcgc caatgtaacc
    27721 gagaaatgaa gtgtaaatac cgaggagaag attaccgatg agaactgcga ggaagaaatc
    27781 atgatagcta agaccggttc cgagagtgcc gccggtccac atactggcgg aaaagaaggt
    27841 taatcccagc atgacgaacg tcaatgccaa tacccctttc cgcgccgact gtgggactgg
    27901 cccctggcta aagttgttat cttgcgacac gaaattcccc ctttttgctg tttgaaaccc
    27961 aaaaaatccg ccgcattcta ttcatctgaa gataaaaagc aatcgttttc gtggggaaat
    28021 atatttttta tatgaatgag tgcaaaaagg tgggggaaaa attatcggtg taaaaacagt
    28081 gggtaggcat aataagacgc ggtaagcgtc gcatcaggca tcggcgcatg gtgtcggatg
    28141 cgccacgcga cgcatccgac aataccttac tcttccatcg cctggcggat ctgatccaat
    28201 gacgcaggat catcaatggt cgtcagatcc ccaggatcgc gtccttcgca aattgcctgg
    28261 atcgtgcggc gcagcatttt tccggatcgc gttttcggca attgcgagac aaaccagacg
    28321 tgcgccgggc gaccaaagtt gccaatctgg ctgtccacca gcgccataat cgccttctct
    28381 tgcgaatgcg ccacatcacg atcttccaga ctatcgctct ctttcggaat gacaaacgcc
    28441 accgccacct gccctttcag cgcatctttc accccaacca ccgccacttc ggcaacgccc
    28501 ggatggctgg agatactctc ttcaatctcg cgcgtgccca gacgatgccc ggcaacgtta
    28561 attacatcgt cagtgcgccc gagaataaag tgataaccgt cagcgtcacg gatgccccag
    28621 tcaaaggtgg cgtacaccgg gcgggaaaac agcgaccagt aagtcttcac aaagcggtcg
    28681 tcgtcgcccc agatggtctg aatacagccc ggcggcagcg gcccttccac caccagcatc
    28741 cctttctcgt tgacgccaca cggttcgccg gtgacttcat taagcaactg cacgttatag
    28801 ccatacatcg gcacgccggg gcttcccaga cgcgtcggcc tgtcgtccag accgcgagca
    28861 atcgccataa tcggccagcc ggattcggtc tgccagtagt tgtcgatgac cggcacatcc
    28921 agcgtattgc tcacccaact ggcggtcggc tcgtccagcg gttctccagc cagatagagc
    28981 acttccagcg acgagagatc gtgtttgcga atttcagcgg tagggaattt tttcagcacg
    29041 cgaatggcgg tcggcgctga gaacatccgg ctaacctgat atttctcgac gattttccac
    29101 cacacgccgc agtccggcca ggtcggcaat ccttcgtaaa cgatagtcgc catccccgcc
    29161 agcagcggcg cgtaaacgat atacgaatgc cccaccaccc agccgatatc cgatgcgcaa
    29221 aagaacacgc tgcccgcttt gccgccaaaa atggtgtcca tcgaggtcgc cagcgccacc
    29281 gcatatccgc cgacgtcacg ctgcacgcct ttaggtttgc cggtcgtgcc ggaagtgtag
    29341 agaatgcagg aggtttcgtt ggattccagc cacgccactg gtacccgcgc gccgatgtgt
    29401 tgatagcgca acgacgcgaa atcgacatcc cgcccgctga cgcgcgccat tttcgccagt
    29461 ccgcgatcca ccagcaaaac atggcgtggc tggtgctgcg cctgacttat cgcatcgtcg
    29521 agcaattttt tatagggaat gattttgcca ccgcgcgctc cggcatcagc cgaaacaatc
    29581 agcaccggtt tagcatcatc aattcgcgcc gccacgctgt gcgaggcaaa tccaccaaac
    29641 accaccgagt gaatagcgcc aatgcgcgcg caggccagca gagtaatatg cgcttcggca
    29701 atcatcggca tatacaccag cacccgatcg ccacgctgca cgcccaatga acgcaacatt
    29761 gacgccaccg cgtttacttc gtcatgaagc tgacgaaagg taaaggtgcg ctcttcttct
    29821 gtttccgaag agacggcaat cagcgccagc gcctctggtt gtttctccag ccagcggtcg
    29881 atagcgttgt ggcacaagtt ggttcggcct tcacaaaacc aacgggcaaa cggcggattg
    29941 ctgtggtcga gcgtttgcgt aaagggcgtc tgccagtcaa tacgccgggc ctgctcggcc
    30001 cagaactgct ccggttcgtt aatcgaacgc tgataaaatt cgctaaaaga cataatgctc
    30061 tcctgttgaa cttacgcctt accgccgttt acttaaatga cgtacaggtc gagatactca
    30121 ttgaccggca tctgttccag gcgagttctg tcgagagaaa cctccagaat gcgctgctgt
    30181 tggcgagtcg ggaactggcg cgcgagattg attttgaatt tatcgaccag tttcggaata
    30241 ccatcctgac ggcggcgagc atgaccaatg gggtactcca ccaccacttc ttcaaatcgt
    30301 gtgccgtcgg tgaactcaag ggtaatggca ttggcgatgg cgcgtttttc cgggtcgtgg
    30361 tagtcagcgg taaatgccgg atcttcaaag caattgatct tctcgcgcag ggcgtcgatg
    30421 cgtttatctt gcgcaacgtt gtcctcgtaa tctgccgccg ttaagcgccc gaataacagc
    30481 gggatcgcca ccatgtactg aatgcagtgg tcgcggtcag ccgggttatt gagcggtcct
    30541 tttttgtcga tgatgcgaat acaggcttcg tgggtgcgaa tggtcacttt ttcgatatcc
    30601 gccgccgtct tgcctgctgc ctgcatctgt tcatagagcg tcatcgccgc ttcaactgcc
    30661 gtctgggagt ggaactccgc cgggaaggag attttgaaca gcacattctc catgacgtaa
    30721 gaaccgtacg gacgctggaa gcggaacgat tcacctttaa aggagacgtc gtagaagccc
    30781 cataccggcg cggtcagggc tgacgggtaa cccatttcgc ccgttttcgc catcagtgcc
    30841 agacgtaccg cgcgggaagt ggcatcgccc gccgcccagg atttacgcgt gccggtgttc
    30901 ggcgcatggc gataggtgcg cagcgactga ccgtccaccc acgccagcga aacggcgttg
    30961 agaatttcct cgcgggtcag gccaagcatt tcggcgacca cggcggtgga agccactttc
    31021 actaacagaa cgtggtcgag gccaacgcgg ttaaaggagt tttccagcgc gatgcagccc
    31081 tgaatttcat gggctttgat cattccggtc agcacctgtt tcatggtcaa cggcgctttg
    31141 ccgctggcga ccgcgttgcg cgaaagccag tccgccgttg ccagaatgcc gccgaggttg
    31201 tcggaaggat gcccccactc cgccgccagc caggtatcgt tgaaatcgag ccaacggatc
    31261 atcgcgccga tgttaaatgc cgcctggacg gggtcgagct ggaactgagt tccggggacg
    31321 cgcacgccgt taggtacgac ggtgccgggg acaattggcc ccagcagttt tttacaggct
    31381 gggtattcga gagcttcaag accgcagccg agcgtgtcga gcaggcagta atgtgcggtg
    31441 tcgtaggcta ctttggagct gatttcgtag ttcatgacgt aatcgacgat atcaacgatt
    31501 tcacgatcaa attccgggcg gatgttgttg atttgagctg acattgggta cgtttcctta
    31561 ttgttattcg tagaggttta ctggcgctta tccagcgcga caaactggcg gtcttccggt
    31621 ccaacataat tggcggaagg acggataatt ttgttgtcct gacgttgttc gataatgtgc
    31681 gccgcccagc cggtgacgcg ggcgataaca aacagtggtg tgaacatctc ggtgggaacg
    31741 cccatcatgt tgtaggaaac agcggagaac cagtcgagat tggggaacat ctttttgctc
    31801 tcccacatca ccgtttccag gcgatcggcg atgttgtaca tcttcagcga gccgccttcc
    31861 tgcgagagct gcttcgccac acgtttaatc acctggtggc gcgggtcggc gatggtataa
    31921 accggatgac caaaaccaat gaccacttct ttgctttcca cccgcttgcg gatatcggct
    31981 tcggcttcgt ccggcgtttc gtagcgttgc tggatctcca gcgacacttc attcgccccg
    32041 ccgtgcttcg gcccgcgcag tgcgccaatc gcgccaataa tcgcggaata catatcagag
    32101 ccagtgcccg caatcacccg gctggtaaag gtggaggcgt taaactcgtg ttcggcgtac
    32161 agcaccagcg aaatatgcat cgccttttcc cagctttgcg acggcttttc gccgtgcagc
    32221 agatgcagga agtgaccacc gatggagtcg tcatcggttt ccggctggat gcgttcgccg
    32281 ttgtggctgt agtgatacca gtagagaaga atcgagctaa gtgacgccag cagtttgtcg
    32341 gcaatatccc gcgcgccaga gacggtatgc ccctcttttt ctggcagcgt gcagccgagc
    32401 gcggaaacgc cggtgcgcat aacatccatc gggtgtgacg ccgccggtaa ggcttccagc
    32461 acggtacgca cgttagccgg taaaccgcgc agggctttca gtttcgtttt gtaggcggcg
    32521 agttcgtcac gggtcggcag tttgccgtgg atcagcagat gcgccacttc ttcaaattcg
    32581 caatgttccg ccagatcaag aatatcgtag ccacggtaat gcaggtcatt gccactttta
    32641 cccacggtgc agagcgccgt attgcccgcc ggaacgccag aaagtgccac agattttttc
    32701 ggtttaatga catgggtact gttttgcagg atcgttgtgt cgctcatatt gtcctcgtca
    32761 tttgtagggt cgagatattt ttcgttgtgt tttatttcac ctggctacgg gcaaacaggt
    32821 cgtcgagctt ctcttcgtac tggtagtagt tgatgctttc gtacagctcg ttgcgggttt
    32881 gcatggtatc gatgacgctt ttctgcgtgc cttcctgacg caggacgttg tagacatgtt
    32941 cagcggcgcg gttcatggcg cggaacgctg aaagcgggta cagcgccatt gcgacatggg
    33001 cgctgcgtaa ttcgtcggtg gtaaacagcg gtgttgcgcc aaattcggta atgttggcga
    33061 ggattggcac ctgcaccgca tcggcaaact ggcggtacat ggcgagttcg gtaatcgcct
    33121 ccgggaacaa catctcggca cccgcttcaa cataggcctg cgcacgctcg atcgccgcat
    33181 ccagcccctc taccgccaga gcatcggtgc gcgccatgat cacaaaatca ggatcggttt
    33241 tcgcatccac cgccgcgcgg atccgatcca ccatctcttc tttcgagacg atcgctttat
    33301 tcggacgatg accgcagcgt ttcgcaccaa cctgatcttc aatatgcaat cctgccgcac
    33361 cggctttaat catcgatttc acggtgcgcg ccacgttaaa ggccgaagaa ccaaaaccga
    33421 tatccgcatc caccagcagc ggcagcgaac aaacgtcggt gatacggcga atgtcggtca
    33481 gcacatcatc aagggtagaa ataccgagat cgggcagccc cagcgaacct gccgccacgc
    33541 caccgccaga aagataaatt gcctgatatc cggcacgctg cgccaacagc gcatgattag
    33601 cgttgatggt gccaacaatc tgcaatggat tttctttagt cagtgcagcg cgaaacgctt
    33661 tacctggaga gtgtagagac atagcccatc ctttgttatc aacttgttat ttgcgttgat
    33721 aaagacaaag caaggggtgt gccacaattg ccttaataaa attaaatgtt taatttcaat
    33781 ttgttagaga taaagtcatg aactaatccg caaatatgcg tttcagttaa cgtttcaggc
    33841 aatgtttcac cgcgtttcat tgcaacaatt atgaaacaag actaaaccca atattcggtt
    33901 tcttaacttt gcggtgcgct atggcacatc caccacggct taatgacgac aaaccggtta
    33961 tctggacggt atctgtaacg cgcctgttcg agctgtttcg cgatatcagc ctcgagtttg
    34021 atcacctggc gaacattacc cctattcagc ttggctttga aaaagcagtg acctacatcc
    34081 gcaagaaact ggcaaacgaa cgctgtgacg ccatcatcgc ggctggctct aacggcgcgt
    34141 acctgaaaag ccgcctgtca gtgccagtta ttttgattaa accgagcggc tacgatgtgt
    34201 tacaggcact ggcaaaagcc ggaaaactca cctcttctat cggcgttgtc acctatcagg
    34261 aaaccattcc agcgctggtg gcgtttcaaa aaacctttaa tttgcgcctc gatcaacgta
    34321 gctacattac cgaagaagac gcacgcgggc agattaacga gctaaaagct aacggcaccg
    34381 aagcggtggt cggcgcgggg ctgattaccg atctggcaga agaagccgga atgaccggaa
    34441 tttttatcta ttccgccgcc accgtgcgcc aggcgttcag cgatgcgctg gatatgacgc
    34501 gcatgtcgtt acgccataac actcacgatg ccacccgcaa cgccctgaga actcgttacg
    34561 tgctgggcga tatgctcggt caatcaccac agatggaaca agtacggcag actattttgc
    34621 tgtatgcccg ctccagtgca gcggtgttga ttgaggggga aacggggacg ggcaaagagc
    34681 tggcggccca ggcgattcat cgggaatatt ttgcccgcca cgatgcgcga cagggcaaaa
    34741 agtcgcatcc gtttgttgca gtcaactgcg gggcgattgc cgaatcgctg ctggaagcag
    34801 aactgtttgg ctatgaggaa ggggcgttta ccggctcgcg acgcggcggt cgcgccgggc
    34861 tgtttgaaat cgcccacgga ggtacgctgt ttctcgatga gattggcgaa atgccgctgc
    34921 cgttgcagac ccggctgctg cgggtgctgg aagaaaaaga ggtcacccgc gtcggcgggc
    34981 atcagcctgt tccggtggat gtgcgggtca ttagcgccac tcactgcaat ctggaagaag
    35041 atatgcggca agggcagttt cgccgtgacc tgttttatcg gctgagtatt ttgcgtctgc
    35101 aattgccacc actgcgcgag cgggtggcgg atattctgcc actggcggaa agctttttga
    35161 aagtgtctct ggcggcgctc tccgccccgt tttctgccgc attacgccag ggattacagg
    35221 caagcgaaac cgtgctggtg cactacgact ggccgggcaa tattcgtgaa ctgcgcaata
    35281 tgatggagcg actggcgcta tttttaagtg tggaaccgac gccggattta acgccgcaat
    35341 ttttgcagct gctactgccg gaactggcgc gcgagtcggc gaaaactccc gctccacgct
    35401 tactgacacc acaacaggca ctggagaaat ttaatggcga taaaacagca gcggcgaatt
    35461 atttaggcat cagccggacg acgttctggc ggcggctgaa aagctgaact gccgaatgca
    35521 gcgcgaacgc tttatccggc ataaatttca atagatgatc accgataaac ttacgcatcg
    35581 ggcatgttta catagggttc agaattactt cttcttataa atatttgccg tgccgtggat
    35641 cttattgtca gtttgaccgg aggtcagtac caacacatca gccccttttt catccgcttt
    35701 tttgatcaaa tcttctttcg cgtctgcagt cgacatttca ttgctggttg aaatatcacc
    35761 tattttttca tactgcgatt caactttttc aaattccgct ttggtcatca attcagcggc
    35821 atagacattg gtaacggcta acgctaacgc accgactaac attttagaga ttattttcat
    35881 attacggctc cagagttgtt atcacactat cacccaccca cattattggg ttgattaata
    35941 attagttcag tcagggcata aacgccagta accggaaata agacactttg tgatctctct
    36001 cgcacccgat tatttttggt agaaatatga tgaagttact tcacttattt atggatgaaa
    36061 tcactatgga tcctttgcac gccgtttacc tgaccgtagg actgttcgtg attacttttt
    36121 ttaatccggg agccaatctc tttgtggtag tacaaaccag cctggcttcc ggtcgacgcg
    36181 caggggtgct gaccgggctg ggcgtggcgc tgggcgatgc attttattcc gggttgggtt
    36241 tgtttggtct tgcaacgcta attacgcagt gtgaggagat tttttcgctt atcagaatcg
    36301 tcggcggcgc ttatctctta tggtttgcgt ggtgcagcat gcgccgccag tcaacaccgc
    36361 aaatgagcac actacaacaa ccgattagcg ccccctggta tgtctttttt cgccgtggat
    36421 taattaccga tctctctaac ccacaaaccg ttttattttt tattagtatt ttctcagtaa
    36481 cattaaatgc cgaaacacca acatgggcac gtttaatggc ctgggcgggg attgtgctcg
    36541 catcagttat ctggcgagtc tttcttagtc aggcgttttc tttgcccgct gtgcgtcgtg
    36601 cttatgggcg tatgcaacgc gttgccagtc gggttattgg tgcaattatt ggtgtattcg
    36661 cgctacgcct gatttacgaa ggggtgacgc agcggtgagc agataattta ctctctacgt
    36721 aatcaacctg atttgccgaa tcccggccat cggcgatgat agcccccgta atgcgtggtc
    36781 cttatttccg ataagtttac ttgagtcttt agcttcccat ttcagcccac tctcccctgt
    36841 gtgggctttt ttcattggtc tgacccgtcc tgaatagcca aacaatggca atgttccact
    36901 ttgcagggat gataataagg ggatctgatt cagaagagtt ttgaaaagtt ggaccgccat
    36961 cgaggcctcg aaccccgtac catacaacaa tcctggttgt cgtctgctct tcctgatgag
    37021 ctaatggcgg tttttgatat gagccttaca gcatattttc tgtcaggctt gagttcgatg
    37081 ctaaggtaca gaaaagaccc cacacaaaca tgatggaatt atgatttttt tcatatgaat
    37141 gtcaacccac acattcacaa aacatctgca tgagatcatt ttttatacac cgcatagctg
    37201 cgaaaccact gctggtgctt ttttaagcga agcgtcgccg atcacaacaa aatatcggcg
    37261 aaaaaggcgg tggaatgcca tttggcatac ggatatacag atgaaatgat tacctgcttt
    37321 gcgattcact ctctggtgga ataacaactt gcaggtggaa ccctttctga actaaagagt
    37381 gtagcggatt ttaacgtgct tatttacagg ataatatctg tcacctaatt cctgcactct
    37441 attcgatatc cattcattta atcgtgcatc atctctgtat gtttttacgt ccagaacaga
    37501 gagcaataac atcaccagtg attcaggaaa aacagggtag atatttttta agtgtaataa
    37561 taatccatgc gcaagcgcct gcttaatggg cgcgtctgtc tccggaatag acgacatttc
    37621 gcgaagccat ttttccacac gcatacactc tttcccttta gggaaaattc tcagcaaagg
    37681 catatggtgt tcaagaagaa tacatttgca atctgttcgc actgaatcga taaactgcac
    37741 taatttctta agcccggtaa agcgtccagc ctgcacattt gctttaatat caagaaacgc
    37801 atcatcctgg tcatataccc aaactgtccc tttttgattt tcaagtatgc gaaaaacaaa
    37861 cttaaccggg tgatcagggt aatccatcca gtcataaaat cctgggaaat cattaaactg
    37921 cacatttatg gtgagtgcgt tatcgtctga ccactcacag tctcgtacgg cagccatgag
    37981 atgaatgagt ttgtcgtcat cgactaaatc atcacgacaa tcataaggat aatcataata
    38041 ttgtttaatc aaaaccttga atatatcgac acaggaaacc agggagcttt tgatatcctc
    38101 tggtaactga ttgtttttaa ccgtattaag tatgctttgt tgcgtatatg gcatcaaatt
    38161 attgtgcgga gcttttaatg atatcatcgc gatctctctt aaataaaaat aaacgtcgca
    38221 gataataaag cgctgattaa accaggcact atcagaaatc gctcataact aacgtgtgaa
    38281 gtattgtgta ctggagggag ttaatttaac aatttgaatt tacgttatag gttttactgc
    38341 tactttgata ttatttcgct ctccttaact aaaaagttaa gtggagagcg aagttaatat
    38401 gttaaaccac agcgtattta ttaatttttt cagtctgtta gtgtgcgatt atcgataaca
    38461 aaacgatatt tcacatcacc acgcagcatt cgctcatagg cttcattaat ttgatcggcc
    38521 cgaatcatct ctatatcagc cacgatgcca tgttcggcgc aaaaatcgag catctcctga
    38581 gtttctggaa tgccgccaat catagaaccg gctatcgcac ggcgtttcat gatcaggttg
    38641 aaaacttccg gcgatttatg cggtgtcgca ggcgcaccaa ccagcgtcat ggtgccatca
    38701 cgcttcagca aggtggtaaa atcgtcgaga ttatgtggcg cagctactgt attcaaaatg
    38761 aaatcgaaac tcttcagatg agccgccatc tcatcggcat tgcgtgagtt aacaacttca
    38821 tcggccccca gggcttttgc cgcttcgcgt tttgcctcag aagtggtaaa tgccaccaca
    38881 tgcgccccca tcgcgtgggc cagcttgatc cccatatgtc ccagaccgcc gatgccgacc
    38941 acgcccactt ttttacccgg cccggcctgc cagtgacgta gcggcgaata cgtggtgatc
    39001 cctgcacaca acaaaggagc caccgccgcc agctgctctt gcgggtgacg aatacgcaga
    39061 acatatcgct catgaacgac gatctgttgt gagtagccgc ccagagtatg gcccggttcg
    39121 tccggcgtcg gcgagttata ggtgccggtc atgtgatcac agtagttttc cagcccgtct
    39181 tcacactctt cgcaatgttt acaactgtcg acaatgcagc cgacaccgac cagatcgccc
    39241 ggcgcatgtt tttctacctg atcaccaacg gctaccacac gccccacaat ttcatgaccc
    39301 ggtacgcagg ggtaaaccgt ccccgcccac tcggaacgga cctggtggag atcggaatgg
    39361 cagacgccac agtaagcgat ttcgattttg acatcattcg gtcccggttc acgccgtgtg
    39421 atatccatcg gttcaagtgg ttgtttagcg gaatatgcac caacagcttt gatcttcatt
    39481 gtgtttactc ctgattagct atgtgtattt ggcgacgcct gatatgtcat cgcggcaaaa
    39541 cgcgtccatt gaatatagcc aatatttaaa ccacccgatc cagccccaac atgccgcgaa
    39601 cctgaatgaa acagagcagc aacgttcccc ataaggccat cgtcaggtag gggcttatac
    39661 cgagaatatt gaagccgctt tcaataattt gcagaagaaa cagcgccagc accataccaa
    39721 ttatccgccc gctgccgccg tccggattaa ccccaccgag tactgccgcg aggattgaca
    39781 ccagcaagta cgattcgcca taagaggcct tagccgagtt gagtttcgac atcatcaaaa
    39841 atgccgctac cgcacaaagc aaggcggaaa tgacataaac ccaaatcagc acccgacgag
    39901 tgttaatgcc ggagtaaagt gttgctcgtt cgttagaccc aatcaggtaa atggctttac
    39961 cgagcggcgt tttctccagc aatatccata gccccagtgc cactgcggca aacaaccaca
    40021 tcggtagcgg aataccgaac cattgcgcat gattcaacca cagcacccat gagggataat
    40081 tggcaatggc gctaccacct gtcaccagaa tatttatccc tttgagcaac gtcatcatcc
    40141 caagcgttgc aagaattggc gacactcgaa taccagcaat caatacgccg ttacataaac
    40201 cgattatcac cgccgcgcca gcgcccgcca gtaacgtagc gacaaccgta gcaatacccg
    40261 gtggatactg agtcgccacc cacgccatca ccagtgaaca ggcgttagca gtggctataa
    40321 ttgacaagtt aatgccaccg caaagcatgg tcaccgccat agccaacgcc agaatgccca
    40381 gcacgggcat ttgcgaagcg acggactgga aattacttat cgaccagaaa atctccggca
    40441 tagtaatgct gaacgcggca acagttagca ccagcaggcc gatcaagtaa aactcaacgt
    40501 tattacgcca cgattttttc atagcagact ccggttctga tgctgactcc acgccgtggc
    40561 actaatgctg gcaacgatga tgatgccggt gatcaatgtt tgccagtaag acgagactcc
    40621 caataaattt agcccgtttt gcatcactgc caacaacacc acgccgagca aagtaccggt
    40681 taacgtgccg cgcccgccga gcaaactagt gccgccaagc accaccgcag ccagtactgt
    40741 cagctcataa cccagaagag aatcgggggc gacagtcatc accgtccacg actgcactac
    40801 acccgcagcg ccagacatca atcccatata gccatagacg aaaagttgca gtttcagcac
    40861 gctaaagccg atgcgactgg ctgattcgcg gttcccgcca agggcataaa ttttgcgccc
    40921 tacactggtg aaattcatga taaacgctgt tagcagcacc accgtaatca tggcgatcag
    40981 cggcagacca aggccatagt catagccatc agcatcggtg tacttgaaca acataacccc
    41041 ctgctcaaac cacggcggaa agtcgtacag ccacacacct ttactcaacc ataacagcag
    41101 gccatagaaa atattcagcg tcgagatagt gatgatgata gaaggcaccc gtaggcgatt
    41161 aaccagtaag gcgttaatta agccaagtaa aatgccgatg cagcccgcca gtgcaaacgc
    41221 gacagcgaag ttgccaccaa ttttttgcag caacagcacc atgccgtatt gcgcaatgat
    41281 ggtcattgct ggaaacgaaa tatcaattcc accggaaatc aacaccacaa acaaaccaca
    41341 ggcgagaata gtcaacatgg catagttatt ggcgagatcg tacaaattac cgaatgtcag
    41401 aaactcgtcg ctgcgccagg caaggccaac aaacagcacc acaatcagta atgccagcca
    41461 gaattcatgg cgttttttca gttcagccat ttaccacctc cgcaatcctg gcctgagaac
    41521 tgctgtcagg caggaagcta tgggtgatct gacctttttg cattaccaaa atccggtggc
    41581 tttgatacca cgcttcttca atttcatcgc agatcatcaa taccgcaatg ccatgagccg
    41641 ccagatcgct gataatgccg tatattcctg ctttgttggc gatatcaaca ccgactgtcg
    41701 ggctatcgag aatcaacagt cgtggcccaa tcgccagcca tttggcgatc gatactcgct
    41761 gcgcgttacc accagaaagc gtattcaccg gaagatgagg atcagagact ttgatggtca
    41821 atgccttgat tagcctgtcc accaggtcac gaactttagt ttcactcaga aagccgaagc
    41881 gatcttttac cttgtgaaaa acagtgctaa tgatgttgtc ttcaatagat tgcgccatca
    41941 ccaaaccgcg cgacatgcga tcttccgaaa cataaccaat accgtggcgg atagcgtcct
    42001 ggttactatg cagtgtaacc ggctggccgt tgatgaggat ctcgccagca tccggtcggg
    42061 tcataccaaa cagactcaga cataattccg tgcgccccgc accaagcagg ccaacaatcg
    42121 ataccacttc gcccgcttcc acccgcaagt taatatttag atattcccca tgacgactta
    42181 aattacgcac ctcaagcacc ggggtgctgg attttccctg ccacaactct cgcacctgat
    42241 attcgaactc ctggccggtc atcaggaaac cgagttgttt tgtggtcatc tctgcagccg
    42301 gaaaagtccc gaccagctca ccatctttca gcactgaaat acggtcggaa acttccatca
    42361 cttcttccag acgatgactg acgaagacca cgcagatccc gcgttcccgc aattgatgca
    42421 ccacctgtaa taacccttgc acttcctgat gggtcagcga agcggttggt tcatccatca
    42481 cgatcaaccg tgcgtcctgc gccagtgcac ggcaaattgc taccagctga cagcgggcaa
    42541 ttgaaagctc agaaacgagt gtatccagtg gcaatgtgac gttaatactg gtcattgccg
    42601 cctgcgccac ctcacgcaga cgacggcggt taacaaacag accgtggtga tagtgattca
    42661 cggcgatatt ctcccagaca ctcaggttag gaaataaaga gaggtcctga taaatcacct
    42721 gaatcccctg cgccaccgaa gcggcgggtg tcagctttga ccagtttttc ccaccaagcg
    42781 taatttccgc tccctcatcc gggcgataaa caccggacat aattttaatc aacgtcgatt
    42841 taccacagcc gttttgcccg gcaaggcagt gcacttcacc cgacataaag tcgagagaaa
    42901 catcgcgcag cgctcgcgtg gcatgaaacg ttttgttgat atgacgaagg gaaaggaaag
    42961 tttccataac atcccctggc aataagaatc ggggctttcg ccccgaagaa atggattaat
    43021 aaagtgagtc gatattgtct ttgttgacca gtaatacctt atggaaacgg ataattcgct
    43081 tatccatatc tacgtcggcc ttacccagat tctgcatttc taatccaggt ttgatctctt
    43141 cacccttcag caacgtgctg gcgacggctg cgagtgcgta acctgcagag gccggatcgt
    43201 aagtaatccc ttcggtgata tcaccacttt taatcagtga tgccgcctgt gaagggatca
    43261 tcatgccata gactgcgact ttatttttcg cccgtttctc tttcaccgca cgtcccgcgc
    43321 caatcggacc gtttgaacca aaggagacaa ccgctttcaa gtcaggatag gttttcatca
    43381 ggtccagtgt ggtacgacgt gagtcatcca cactctcggc aaccggcatg cggcgggtaa
    43441 cttcatgcat atccgggtaa tgctctttct ggtatttcac cagcaagtca gcccataagt
    43501 tatgctgcgg cacggtcaaa ctacccacgt aaatcacata gccgcccttg ccccccatgc
    43561 gtttcgccat atgctcaaca tattcagcgg caaatttttc gttatcaatg atttcaatat
    43621 cccagttagc acttggctga ccgggggatt cgttggtcag aaccacaatt ccggcatctc
    43681 gcgctttttt gaataccggt tccagcacgt tggcatcgtt tggcacgata gtaattgcat
    43741 taaccttacg ggcgattaaa tcctcaataa ttttaacttg ttgcggagca tcagtacttg
    43801 aaggccccac ctgtgaggca ttaacaccaa aggctttacc cgcctcaacc acaccttcgc
    43861 ccatgcgatt aaaccacggc ataccatcga ctttagaaat attcaccacg actttttccg
    43921 ctgcctggag cggcgcagaa attagcgcag cgcctaataa cagcgaagac accatattga
    43981 taacaaaacg tttattcatc atttcagcct cattgcaggg tagtcaggaa gtgttcgccc
    44041 aaattagcgc gctggagaga ataagaattt tcgaatattt ttatgcagaa taaacagccc
    44101 tatttcaggc agctattaaa aatatggtta taaatatgga caaaacgggc agcaacaata
    44161 agtgtgacat tcatcacgta aaaaatagca aaataaacaa aattagcgat attttatgtg
    44221 tgtgagttta tttcgcgtag ataaatagtt atctacacaa cgtcaataag ggcatattct
    44281 tcaaatataa cgtccatttc tggtgaatag cttccttctg cctggaaaaa acgacggtgt
    44341 tcattacgcc aatattcaag actgaggtca ccttcacctt ccttacgggc aagctccacg
    44401 gtgacatcag aaaagcgcag taaatgcagg ccaatcactc tgataacaca tacaggctca
    44461 ccacggctat tttctacaat cttatactca ccaatcatcg gaaatgcgtt gtcttcaata
    44521 catcccgcca gcgaaccaca ggcagctgtt ttaacaccag tcgcaatcaa cgttgcgagg
    44581 tgatcggcca acgctgctga atcgccaaaa gtacaaaagg aagcatgggg atatttttgt
    44641 gagaattttt cttttatggt catggcaata tttttgcgat tacggaaaaa caaatcacat
    44701 cttaattact gttggtaacc atcagcaaga gtatcattgg tttttcgata ctgaatcttt
    44761 tttatccgaa gcaggtcaca tttatggcct gataaatacc tttgttaaaa ctcacttttg
    44821 tgtatttctc tcaaataatc ccctggaaat tatgccgcga gattataaag ttcaggatca
    44881 ttcattagca aaccattccc gccccactcc ctggggctta aatctcttac aattcaccgt
    44941 tgcaggcgtt tcaaatgcgc atcaacctgc cacactcccc cacaccagtt gccctttatg
    45001 gaacgttgcg gttctcggcg agatacgcgc caccgcctcg gcggaacagg aggcgtccac
    45061 cagcacaaag ctggcatcat cctgcgcttt tggccataca cgctcgcctt tttcattcag
    45121 cggcaataca tcgccagtgg ctagaaacag cgaacgggag aggttctgtt cgttaggacg
    45181 aatatagagc tgcgcgtaga gattggcttt ttccagcatg tcgcccagac cataaggcga
    45241 ccagtggtcg ataacgctgt cagtgccagt catcactttt acgcctttgt cgtgcaactg
    45301 tttgagcggc atatgcagcg tgccaatcgg caccgtcgag gcgatagaaa tttgctgcgc
    45361 cgccatccga ttcgccagcg catcgacctg ttgttcgttg agcgtagcca gcgcaaaggc
    45421 gtggctgatg gtcagcttac ctttcagttg cggcgttttc tctaccgttt caaccatata
    45481 attgatggct gccacgcccg acggagtggt ttcgtgcagg tgaatatcga cacctttgtc
    45541 gtagtccagc gcaatctgaa acatggtgtc gagggatttt tccatcgcgc catcgacact
    45601 ggtcgggtcc agcccgccga cgtaatgcgc acccgcctgc atcgcttcgc gcattaaggg
    45661 ttccgatttc gacagcagca aaccgtgctg cgggaaggcg acgatttcac actcgaagcc
    45721 cgcctgacgt cgcgccagca ccgcctgcaa attctgcaga tttttcaggc cggaaaccgg
    45781 ttcgatattg cagtggctgc gggcaatggt ggtgcctttc gactgcaata gatcgatcag
    45841 tttttctgcc cgttcctgcg tgtacggttg cagttccggc agcattttct gctcaagctt
    45901 gatcatgtcc tggatagtgg tgcctgccgg acgattgagc gagcgccacg gcccgccgta
    45961 gaaggttttg tcgagatgaa tatgcatgtc gcgagtggtg ggtagcatca acttaccgcc
    46021 agcgtcatag tgcggcagcg tggcgtccgg atgctgcttg ttctcgcgca gggcgacaat
    46081 tttgccgtcc tgaatctcca cggtctggcg cgccgtgcgg gtctgcaccg ccacaccgtt
    46141 ttcgtagtca aaaccggttt ccagcagcac gttatcgaga tagtaatgcg ggtcagtgat
    46201 tttcatggtg ttgttcgctt cgcagtttag ggtgccagct accgccgcat gggcgagcgg
    46261 cacagaggta ccaaacagcg cggcggcggt gaccatctta ccgctctggc tcagaaattc
    46321 acggcggcta ttgctttctt tcatcctacg tccttacgtt ttaataatat gggtgcccgt
    46381 ttcaccgcgc agcgccgcag gcaggcattc cggcgtggtg ataatcactt ctttgccgcc
    46441 ctgctccagg aacgtcaggc tggcgatgat ttttggcaac atgctgccgg gcggaaaatg
    46501 cccttcctgc atatagcgag tcatggtggc aatatccacc cgatccagcg cctgctgctg
    46561 cggtttacca aagtggatac agactttttc gacgccggtg gtgatcacca gaatgtcggc
    46621 gtgaatttca cgggccagca gcgcggtaga gagatctttg tcaataaccg cgtccacgct
    46681 ttggtaatcc cccgcttcag tacgcactac cggaattcca ccgccgcccg cgccaattac
    46741 gacaaaacct tgttggatca gcgccttaat ggcgggtgct tcgacaatac gtttcggttc
    46801 cggcgaggcg accacgcggc gatagccccg tccggcatct tcaacaaaac accagtcagg
    46861 gtttgccttt tgtagtttgt cacgctggct ttcactgaag aatgcaccaa tgggcttggt
    46921 gggatgggca aaacctggat cgtttttatc cacttccacc tgagttacca cggtgacggc
    46981 ttttttctcg ccgtgacgcg ccagccggtt attcagcgcc tgttggatca gatagccgat
    47041 gccgccctgc gtatctgcca cacagttcgc cagcggcgtt aagggcagcc cttcgcgctc
    47101 gtgggcaatc tccgcacggc gtaaatccag cccgacctgc ggcccgttac cgtgggtcag
    47161 cacaatgtcg taatcggaag ccagcatttc cagcaccgta tcggccacgg ctttcaccgc
    47221 ctccgcctga tgctcaatcg actggctggc gttatctttg ataatgctgt tgccaccaat
    47281 ggcaacgacc acaagctctt tcatgttgtt atgtccgcta cagagggtga ttaggttatg
    47341 accaacgcca gactgcctga tgcgctacgc ttatcaggcc tacaattctc gggccatccg
    47401 taggccggat aaagcgttca caccgcatcc ggcataaaca tgtaagtcag acgccgtaac
    47461 gttcgcacca gccgagaatg gctttctcga agcaggcgaa tggtggatgc acaatgcccg
    47521 cgccaatcat gccgacgccc gcatctttat gggcgatggc ggtgttgatc accggcagaa
    47581 tgccgctgct acccacgcgg gtgatgtcga ttgccgacgg cacgcccata aaaccgagca
    47641 gcggaatggt gacgttgggg ttttcgccaa gggtgatttc gcgcatctga cgggagaaat
    47701 cgatagcctc ttccaccgtg ccgcccacca gtgcgacgat tgcaggcgcg gtcgccatgg
    47761 caaacccgcc aataccgtag gtttctgtga tggcgctgtc gccgatatcc agccccgaat
    47821 cttccggctt ataaccagca aacatcgggc cgatgacctg ctgcgctggg ccggtaaacc
    47881 actgccccgg caggccgctg acccgcaggc cgaactcgac gccgttacgc gccatggtgg
    47941 tgaccacggt gctgtattcg atgccgtgcg ccgcatccat cgccgcttta cacatcgcca
    48001 tccacgtcgg gccggagaag tagtcgctgc tggcgacaaa ctcgaacact tcgcgctgtt
    48061 gctcgacgga atagcctgcc tgaatgatcc ccggcgtcag tgcctgaatc agcagcgtcg
    48121 tcccggcgtt attgcggtta tggcactcat cgcccatatg cagcgcctgc gccagcatta
    48181 agcgcagatc gatctcaccg ataattttca tcgcgtcgcg cagcattggc ccctgcacat
    48241 cgcgcatcca gttgaggcgg tcgatcacgc tctgatcgtt ggcacccata cgcaaaatct
    48301 tcgccatctg ctcgctcata ttggtataag cgatgttgcc gtaggttttg tttttcacga
    48361 tgtgcataaa catcgaggcc gaggtaacgc ccgccatcga tcccacgcag tcgtgttcgt
    48421 ggcacggcga gaaggtgatc tccccggagg ccgccagttc agtcgcctca tcgagatctt
    48481 tcgccagccc ttcgaacacc aacgccccgg tgaccgcgcc tttcatcgcg ccgcacattt
    48541 tttcccaggt gatcggcggc ccggcgtgaa gaatggtttt cggcgtcatg cccggcacca
    48601 cgttaatcgc ctgatcaaaa ccaatcagca ccggatgcga ctggataatg cgctccagcg
    48661 cctgctggtt agcggcggcg attttgtctg ccagcggcga atcggcaatg ttatccagcg
    48721 cctgcaccac ctgcatattg ccctgccccg gcggcgtcca gtcgagttga gtcacttcta
    48781 catgctgctt tttcaggtca tcgctaaaca tggcgatacc gacgttaata acgttcaacg
    48841 gttggctaaa cagtgactgg ctcatcaggc ttcctcccct ttgcagataa attcacgcgc
    48901 cagcaatccg gtattggtgc tgctgctcgc cagaatcact ccggcatcaa gcagcatctg
    48961 gctttgttgt tctaatgatg gcgtatcgag atcggtgccc agcacatagg cgagaatgat
    49021 caactcgcgt ccttcggcgg cggcgatcgc tttcgcttct ttgatcgcct cgatggtgga
    49081 gccgaccgga tcttcatgcg atccaaatcc gagcacaaaa tccatcacga tcaccgccac
    49141 ttctggatcg cgcgcttctt cgatcaagcg actgatgcgg ttggtggggt caatcattgg
    49201 atgcggcttg ccgttggtga agtcgtcatc gccaaagtcg aggaaggtgt gtttgatgct
    49261 gcggttgata tcttgcaggc ggaattcagg atcgggctga atgttgctgt agacatcgcc
    49321 atgtttttcc atcaccgcga acatggtttc gtcgcacagc gtgccgccgc agaacaggcc
    49381 acgaatgtat ttctgctgcg gttgcagacg cgcacgcaca tcagcaatca acggctggtt
    49441 aagcgtatgc aggtcgagat tttcctgttt cacgccggag agcatcaccg cttttagcgc
    49501 cgcctctttg ctgccgcggg caaactgtag cccctgctca tccaccggcg tttcaacgcg
    49561 accgaggaag cagaccacca ccggcttgcg gcaggcacgt gcgcgttcca gcactttgcg
    49621 ggccaccgca ggcgcgggag gttttgaaat cagtgcgata atttcagttt gcggatcgtt
    49681 ttccagcatc ccgatggcgt cgagcatcat caggccgccg attttctcgc tcaggtcgcg
    49741 cccgccggtg ccaatcagtt gtgaaacacc gccaccaaat tcatgaatgc ggacgctcag
    49801 ctcctggctg ccggtgccgg atgcgccaac aataccgata ttgccgcgac gcacggcgtt
    49861 accaaaacag agcgccgcgc cgttaataat cgccgtgccg cagtccggcc ccatcatcag
    49921 cagccctttt tcgtgggcca actgcttgag cgccagttca tcttcaactg agacgttatc
    49981 ggaaaacagc atcacgttaa ggtcgttttg cagcgcctga cgcgcttcgc gagcggcaaa
    50041 cagaccgtta accgaaatca ccgccaggtt actttccggg atatgctttt tggcgctggc
    50101 aatagtggcg taacgcgcct cgtgcgagcc gctttgcgct ttgctgttga acagttcttc
    50161 aatctccacc agcaactgtt cgttgtccgc gcccgctttg ccgttgatga caatcatcag
    50221 gtcgccgttt ttcgcctgtt ccagctccgg cgtcagcagt cctaaatttt tcagtacacc
    50281 tttgttcatt tcggtcgcca tcgccacaaa tgcctgctcg acgccgtcga gtttatttgc
    50341 acgcgtggag atagacatca gcgagacaga atcaaaatag gtattcggtt taatgactat
    50401 tttaactgac ataattccct ccgtaagttt ggctcagcgc acaaccatcg gccatgccat
    50461 acagcatgtc gcagccggaa ctggagccaa tatgtttaat tttttctatt gcctgagttg
    50521 cgttcccagg gatgtcataa ataatgttgt gaataaaacg atgaatattt tcccggcaac
    50581 gttgttgtaa tgcggcttcc agcgttatag cacttaataa tgtggtgtta tttctgccgc
    50641 gctgcatacc gagataaaat tcctctttgt atttttccga cggatgcccg ggaataaata
    50701 aaataagcgc cagacctgtt aaataatcgt cggctgatgg cgtaaggcca atccctaacc
    50761 caatcatact ggcgaccgct gcggcgatat tttgtttttc gtttactgcc tgaataactg
    50821 cccgtcgttt aatatgtagt tctttattta acgcctgata aaacggatta tcgccgcgat
    50881 aattaaacag ggtttcattt tcccccaggt gctgatgaat aatgtcgtgc cactgtagcc
    50941 aggcgataag gtgaaaattc acagcggtca tttgcaaggt tggggacagc caacgccgac
    51001 aacgactcat ctctatatga agatgttgac caaccgtaat accttgatcg tcaaactgaa
    51061 ccttatcacc atgccggaac agatcatcaa agtgagtgag tgccaaccga caactgtttg
    51121 gcgcattgtc gtactcttca caaagcagcg tcagcaactg ctggcgagcc gggagtgcga
    51181 tatttaccgc tctggcaaag acctgttcaa cctgtccttg cccccgctgc gccagaaaat
    51241 ccgcatccgc agttagcgcc cacacacacc gtctttctgg tgtgttgata gcaagcctcc
    51301 cggtttatgc acctgcggca atcagtaact gcgcaatctc ttcaaagccc cgttcccgcg
    51361 ccagttccag tggcgttttg ccatatttat cggtcagatg cgggctggca ccgtgttcca
    51421 gcaataactg cacaatcgcc tgctgtttaa taccaccatc attaagcaca atcgcttcca
    51481 gtagcggcgt ccagcctaca tggttggtct ggttaacgtt aatctccgta tgcgccaaaa
    51541 gctcttttac aatacttaaa tggccttttt cacaggcagg cgtcaggccg acaccgccaa
    51601 aacgggttac gcaattaaga tcgggtttag ccggtaaaat aattcgtagt agagttaaat
    51661 catcgttcag acagctaatt aaaaaaggat ttaaacaggt atgatcttgc ttattaatat
    51721 ccgctccggc atcaattaat gcctgaacgc aagcatattg ctgatataaa cttgccagcg
    51781 taattgccgt tttcccctga cgatcgcagg tattaatatc gacacccagc gcaaggcagg
    51841 tttttacttt atcgatatca ccctgttgtg cagccaataa atagtcggca ggtagatttt
    51901 ttatactcat tcacattccc ctggtttttg tggctgtttt cgatgaattg agaatagcaa
    51961 cgataatctg ataaaagaac ccgcttaaat ttaattcgct gtatttcgcc tttttgttga
    52021 atgatgttca acaatctttt cgcagtgtgc ggtagtgcaa tatttgtttc tttgacccga
    52081 taacctttca ctcaatcagg ttttatttct ctatagtctg cacatgtaag agtagctttc
    52141 gtccatgtct aaccagaggg gaacaagatg aacggactca cggcaacggg tgtcaccgtc
    52201 ggtatttgtg cagggctttg gcagttagtg tcttcacatg tcgggctctc tcagggatgg
    52261 gaattattag gaaccattgg cttcgtcgcc ttttgcagtt tttatgccgc aggcggcggt
    52321 aaatcggggt ttatcagaag tctggcggtt aattattccg ggatggtgtg ggcatttttc
    52381 gccgcgctgg ctgccggttg gctggcctct gtaagtggac tgtccgcgtt ctgggcgagc
    52441 gtaattacta ccgtaccctt ctcggcggta gtggtctggc aagggcgttt ctggttactg
    52501 tcctttattc ccggtggctt tctcggcatg acgctgtttt ttgccagcgg gatgaactgg
    52561 acggtgacgt tactgggttt tctggcgggt aactgcgtgg ggataatttc agaatatggt
    52621 ggacaaaaat taagcgaagc gacgactaaa cgcgacgggt attaaaagtt attcaaaaat
    52681 gattatctat ctttaaccga tacctctaca gtcatagctg gagaaattca tagtagataa
    52741 tggcgagaat ggtttaatga actcaatttt taccgaagag aatctgttgg catttactac
    52801 cgctgcgcgt tttggcagct tcagtaaagc cgcagaagag ttgggtttaa ccacttccgc
    52861 cattagctac accatcaagc gtatggagac ggggctggat gtggtgctgt tcactcgcag
    52921 tacccgcagc attgagttaa cggagtccgg gcgttatttt ttccgcaaag ctaccgacct
    52981 gctgaatgat tttcacgcca tcaaacgcag tatcgatact atttcgcagg gcatagaagc
    53041 acgtgtgcgc atttgtatta atcagctgct ttatacgccc aaacacaccg cacgcttgtt
    53101 acaggtgctg aaaaagcagt ttcctacctg ccagattacc gtcaccaccg aagtgtataa
    53161 cggcgtctgg gatgcgatta ttaataatca ggccaatatc gccatcggcg cacccgatac
    53221 actactggac ggcggcggta ttgattacac tgaaattggc gcgattcgct gggcttttgc
    53281 tatcgccccg gatcacccgt tagcctttgt gccggaaccc atcgccgaaa gccaactgcg
    53341 tctctaccct aatattatgg tggaggacac cgcgcatacg attaataaaa aagtgggctg
    53401 gttgctgcac gggcaggagt caattctggt gccagatttc aacaccaaat gtcagtgcca
    53461 gattttaggt gaaggaattg gttttttgcc ggattacatg gtccgtgagg cgatgacgca
    53521 atccctgctg gttacccgac aaatccataa tccgcgccag gattcgcgca tgttactggc
    53581 gactcagcat tctgctacgg gtcaggtgac gcagtggatt aaaaaacaat ttgcgccaaa
    53641 tggcatatta acggggatct atcaggattt actgcatcgg gagaattaat cgctgccaac
    53701 ctcccgatgc gctgtgaata gcggtttaca tcaaccacct gctttcatta cccattcaga
    53761 gataaattta ttacccggta ctggcggaga gtacaaatag ccctgcaaaa agtgaacgcc
    53821 tttaccgatc attaaatccg cctgctcctg ggtttctacc ccttccgcca cgatactcag
    53881 accaggctta cgcgctagtt cgacaatatt gtccacaata tgaccggaga tttcgtcgac
    53941 actcgccatt tgcacaaatg acttatcgat cttaataaaa tcgaccggga acgcctgcaa
    54001 gtaacgatag gtcgcataac ccgtaccaaa gtcatccagc gcaaaggtaa tgttgtgctg
    54061 atgaaggctg tcaaatatcg ctctggcttc tggcgttacc ggaataggat tacgttccgt
    54121 tagctcgaga accagcttga ttttatcgtt acctaatttc ttaaccaggt tcagacactc
    54181 tttttcaaat cccgctgcca aaaaacaacc cgccgagacg ttgatgccaa tatggaaatt
    54241 gtccggcagc aaatgtttta ccggcatcag aatatccgca gtctgtttca tcagttggcg
    54301 ggtcattatg acaataagac cggatgactc cgccagagga ataaactgat ccggtgggat
    54361 aattcccgtt tgtggatgtt cccagcggac aagtacctca cagcccgtca gtaccccagt
    54421 ctgcgcgcag aaaaccggtt ggatccacgg tttgaattcg tggttttcca acgccagact
    54481 gatggcttca ggcgtgacga tatggtgaca acggttatca gggatataaa aattattttt
    54541 atttcccgtg ccactgatga cttgcggatg atattgaaaa aagatatccc gccagaaggt
    54601 aatatcactc tggatgccta gtttttcata cagctgcttc ttttgatgtg aaattgtctt
    54661 tgcactgcga tttctgtact gtgagatttc tgtaaccgac attccctcca ttagcagctt
    54721 gagtaaaagc cgttcttttt cggacacgat accaggcaac gtcaaattca ccgttgcccc
    54781 gaactcttcc aaaaaaacac tcaaatcatg cgaagccatt gaacacacct tgattaaatc
    54841 ttgtgcgcca gctaaatctg cgagtcttca ctctgactga taaagctggc tgcaagaaaa
    54901 tgacacagag agccgaatta tctcccgttc ggctctctct ccttttactc attattcaga
    54961 tcagaattgc cacttaatgc caaccattgc agaggtgtca ttatatcccc tatcggcaac
    55021 ctgaacgccc acattccccc agacattcag gttggcattt agctgtcctt ccaccccggt
    55081 ttttatctca gcaatatttc gggctccatc ctgagtgacg gacacgccat ccatactggt
    55141 gctgaaatcc ttactgttat gcagccagtt cacttctaca aacggctgga actcgcggga
    55201 tttaccgtca tccattttat ggtggctctt cagccaggtt tttacgccaa gtcgggtttg
    55261 aacattgcca tcaccgttgc tatgaacgag ggttccgttg ctttcgcggt gcttatcggc
    55321 tttgactccc atccaggtaa cctgtgcttg cggctgaaca taccattcat tacgcgttcc
    55381 ctggctgcca ttaaattcag ctaatttgtg tttgtatcca gcttccagtg aagcggtaaa
    55441 tccttttgat ttataggatt cactttgtaa gtcatcccct ttcactgtgt tatcaaacca
    55501 gctgtactgc gcccaactgt cgagatacgc gccattacgc gactcgtcat cggcatacca
    55561 ggtggcatag aggccggtgc tatatccgtt cacactcgct tttgcacgat aaccggttcg
    55621 cgaggaaatg gttttactgt cgctgttgcc atatcccgcc atgaccccaa catgccagct
    55681 gtcgctgccg ttttggctcc actgcgcgac atcgcctccc agttgcagaa catagcgatt
    55741 gctttgggtt ttcagctggc cgctgccatc acgccattta ttatgaccac cttcatggcg
    55801 catccacata gaggtttgtt tctgctcacc cgtcaccatg tcggtatagt acgtattacc
    55861 cagacgctca tgcagacgcg tggtgaacat tgtattcgct gctgcaaggt tcgcaatgta
    55921 gctacccgcc tccggtcgca ggtcattatc cacattcaga tccggacccg gcgtaggtgt
    55981 cgggttaggg ttcggctctg gattcgggtt tggctccgga ttcggcatcg ggtctggctc
    56041 cggctgcagt tcaggagaat cactgccgct ggtcagatac cagttaccac tatttgcccc
    56101 ttgtccacgc gcgagagtgt agtcatacgc ccccgcaacg atacgaccag cctgaacaaa
    56161 ttcgccctca gacttaccgt ctacatggat aagttcgata ccgttaaggg ttttcgcacc
    56221 tgtaccgcca gcgttattca ccgtaacggc agtcgtgccg ctagtatcgc cctcgacaac
    56281 caatttatcg gttactgagt catcgctatt cagtaccgta ttcattacta gttgaccgtt
    56341 attaccgtgg tagttcccct tcaccactaa cacattgcca gcttgctgtc ctgacattcc
    56401 ggtcatcagc gtaccgttgt tggtcagatt gccgcccagg gtgtaattca cagacgcagc
    56461 cgctcttttt tcagcgctat tattcaacgc cagggtaccg ttgttctcaa cgtttgcgct
    56521 acccaatccc tgcgcatgac ttaccaggac attagccgaa ttagcaatgt tcacattcgc
    56581 cgtcaggttg ctgttatcgc ccgcgatgtc cagcgtgccg ccctgaatat tcagctctcc
    56641 gctccccgtt aacgaaccgg ttgaagtgcc cccgttggta accgtcagac tgccgccatt
    56701 taagctcagc agtgaatcgg cgctgccatt gagcgtgccc acggtctggc tgtgaccatt
    56761 catatccagt tcagtctctg ccgccagacg cagttcatgg gtattaccca acacgttatc
    56821 gttattcaac aaaagcgtcc cactgcgcag atcggtaaca ccggtatagt cgttgtcttt
    56881 gttagaaagc gatacggtct gacccttctg gctgctgaat gccaggtcac cactgccggt
    56941 aatctttgcg ctgagatcgg cggcattctc gcttttaccg ttagagctca gcaccagagc
    57001 atcgctgtcg gtagcgtgta aatcaagctg ggtcaggccg taaccgatat acagaccgtc
    57061 attgtttacc ccactcgtca gacgatagtc gtaagtgccg acggccgctt tttgcccatt
    57121 ctgctgaata tcacgttggg cactgctgga gaggatttgc ccatttttat cctgcaattg
    57181 cagattgccc gcatcgccca gaacttccgc accttgcgca tcaaccaact taatggtgct
    57241 gttaccatca tcgacttcag tgagcgataa cgcagagtca atatcacgag atactgagct
    57301 tacaacgtca ctgtcagaaa cctgaatcac accctcgccg cgcaaatcca gcgttttact
    57361 aacgttgacc gtcccttctg tcatctgtgc acctgctgtc agggcaccaa aatcaacggt
    57421 tccgccggag aatcccaaag tgctaatgga gtgagcgccc ttacccacta ttacctggct
    57481 tccctgaccc gcagtgatcc cgcctctctg cagaaactca gcattctttt ccaggttaaa
    57541 tgtcgtgttc tggaacgcca gtgaaccctg gaaccagtct gtataagcaa actcatcgaa
    57601 ggagaattcg tgattcccca tgtttatttc aacaatacct ctaccttgaa gatcgttatc
    57661 aaatacccaa tctttattgg cattgatttg cagtttgcca tcaacatcca ggtccgcacg
    57721 gccgatgttc ttgtcttcag aaataaccac gacagcacca gaagcgacat caatatatcc
    57781 gctgaatgca tagttattgc cggtcagtgc cactgtccca ctgtcgatac ttattgtacc
    57841 attgccggaa atcttattgg caaaggtcat gctatcgtca gccttcaggt gtaatttccc
    57901 gcggttgttg atctctgctg tacctatatt ttgggtgatc cctaaagcgc cattttgctc
    57961 aattttgact ttaccactaa aggcagagtt atcaccggtt aagtaagtcg tgccattgag
    58021 cgcattgata tagcccaggc cctcgctgcc actgacaata tccgcggaaa tagtcgcgtt
    58081 tttatccgtg tggtttaagg tgattttctt atcggatgtc tttgcccaca caaactcaat
    58141 tttttcggca tcaataatcc ctgcagcctt cgcagcactt ccttcctgtg cacctaacgc
    58201 taactcagag ttggtgctta aactaatttt cggtgcggat attttacctc catctgatac
    58261 gactaatgtt gtatcagaac gttggtcgta ataacccagg ctaatatgtt cacgagcatt
    58321 aacttcaccg ccgttattaa ctgacaggta ctgattacca ccgtaactgc cgtaaacgcc
    58381 cataaagaag gttttagaat ttacctggct gccttcacca ctgacatttc cctgcaaata
    58441 ggtcagacgg ctggtgccct taacatggat tttgccgccg ttggagacat taaaatagcc
    58501 gcgatcgtgt ctcatgcctg agtttgactc atacaaatca tcagcatata actcgctgcc
    58561 agcattggag acattcagga accccgtacc tttcagcaaa aagttaagtg ggccattgat
    58621 agttaccttt cctccggtaa tatccaaccc accaccagaa gcgagagtca gacgcggacg
    58681 ggaagtatct gtcgcattga ttaccagatt ggcattgtca gtcaccgtaa tacggtaatc
    58741 gatgctctgg ttctctttgc caaaatcaat agttttatcc tggtttacga tgacatcatc
    58801 ggcgaatacc agagatgaat ataaacttat cgcgctaatt aaaacagccc gggacttttt
    58861 tccttttact ctgctcccca gctccgagca gacgctccaa cagttacggg acctgttcca
    58921 ctttagccga tatattttat tcatcacaat ctccagcaat acatccaaat aaaaccaaga
    58981 ttgcataaac agcaacgcat taaatgccat tctggttact gttcatcgca tataaaaatt
    59041 caaaaatttt gcatagcatt ggcaaccaca tatctgagta tgtagttcag agggtaataa
    59101 aatagcaagt tattacttaa aacttatttt aagttaacac tcttaacttt atcattacat
    59161 tcctcccagt ctctaatacc tgcgcattat atgtatccag gaactcaaat caactcaaaa
    59221 tata
//
LOCUS       DAPHDW010000011        57894 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.011, whole genome shotgun sequence.
ACCESSION   DAPHDW010000011 DAPHDW010000000
VERSION     DAPHDW010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57894
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.011"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..440)
                     /gene="folK"
                     /locus_tag="RH057_000660"
     CDS             complement(<1..440)
                     /gene="folK"
                     /locus_tag="RH057_000660"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HDV3127076.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESHILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSLAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREV"
     gene            complement(437..1855)
                     /gene="pcnB"
                     /locus_tag="RH057_000661"
     CDS             complement(437..1855)
                     /gene="pcnB"
                     /locus_tag="RH057_000661"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HDV3127077.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTA"
     gene            complement(1894..2820)
                     /gene="gluQRS"
                     /locus_tag="RH057_000662"
     CDS             complement(1894..2820)
                     /gene="gluQRS"
                     /locus_tag="RH057_000662"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HDV3127078.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHHAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDQLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQISLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWRLTAVPESAIVNSTFSNASC"
     gene            complement(2857..3312)
                     /gene="dksA"
                     /locus_tag="RH057_000663"
     CDS             complement(2857..3312)
                     /gene="dksA"
                     /locus_tag="RH057_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HDV3127079.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3490..4194)
                     /gene="sfsA"
                     /locus_tag="RH057_000664"
     CDS             complement(3490..4194)
                     /gene="sfsA"
                     /locus_tag="RH057_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HDV3127080.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGAERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4209..4739)
                     /gene="thpR"
                     /locus_tag="RH057_000665"
     CDS             complement(4209..4739)
                     /gene="thpR"
                     /locus_tag="RH057_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HDV3127081.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDAREAVTIPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4813..7242
                     /gene="hrpB"
                     /locus_tag="RH057_000666"
     CDS             4813..7242
                     /gene="hrpB"
                     /locus_tag="RH057_000666"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HDV3127082.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGPNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDDAVAVATAEMLRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVLLCPLYGALSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGICLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVSAKNDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIARRRGQDG
                     RYQLANGMGAMLDANDALSRHEWLIAPLLLQGSASPDARILLALLVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLAALETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLELLSPAQRPLQITRDLSAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7438..9972
                     /gene="mrcB"
                     /locus_tag="RH057_000667"
     CDS             7438..9972
                     /gene="mrcB"
                     /locus_tag="RH057_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HDV3127083.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRSKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTDTWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10192..12435
                     /gene="fhuA"
                     /locus_tag="RH057_000668"
     CDS             10192..12435
                     /gene="fhuA"
                     /locus_tag="RH057_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HDV3127084.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDSLD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLLNLYNPVNTDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12486..13283
                     /gene="fhuC"
                     /locus_tag="RH057_000669"
     CDS             12486..13283
                     /gene="fhuC"
                     /locus_tag="RH057_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HDV3127085.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPTEGEILLDTQPLESWSSKAFARKVAYLPQQLPPAEGLTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRGGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13283..14173
                     /gene="fhuD"
                     /locus_tag="RH057_000670"
     CDS             13283..14173
                     /gene="fhuD"
                     /locus_tag="RH057_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HDV3127086.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSPEMLARIAPGRGFNFSDGKQPLAMARKSLTEMADLLNLQSAAETHLAHYED
                     FIRSMKPRFVKRGERPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRAGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14170..16152
                     /gene="fhuB"
                     /locus_tag="RH057_000671"
     CDS             14170..16152
                     /gene="fhuB"
                     /locus_tag="RH057_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HDV3127087.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DINVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPGAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLILAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16187..17467)
                     /gene="hemL"
                     /locus_tag="RH057_000672"
     CDS             complement(16187..17467)
                     /gene="hemL"
                     /locus_tag="RH057_000672"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HDV3127088.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYYGVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGVHETLDELTTRLAEG
                     LLEAAKEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17692..19113
                     /gene="clcA"
                     /locus_tag="RH057_000673"
     CDS             17692..19113
                     /gene="clcA"
                     /locus_tag="RH057_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HDV3127089.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMVLGREGPTVQIGGNIGR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19137..19202
                     /locus_tag="RH057_000674"
     CDS             19137..19202
                     /locus_tag="RH057_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127090.1"
                     /translation="MAIIIGLEFAQLPMSFGAKYE"
     gene            19195..19539
                     /gene="erpA"
                     /locus_tag="RH057_000675"
     CDS             19195..19539
                     /gene="erpA"
                     /locus_tag="RH057_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HDV3127091.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19586..20209)
                     /locus_tag="RH057_000676"
     CDS             complement(19586..20209)
                     /locus_tag="RH057_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HDV3127092.1"
                     /translation="MLVYWLDIVGTAVFAISGVLLAGKLRMDPFGVLVLGVVTAVGGG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GIGVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLAREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20247..21047)
                     /gene="btuF"
                     /locus_tag="RH057_000677"
     CDS             complement(20247..21047)
                     /gene="btuF"
                     /locus_tag="RH057_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HDV3127093.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYAQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21040..21738)
                     /gene="mtnN"
                     /locus_tag="RH057_000678"
     CDS             complement(21040..21738)
                     /gene="mtnN"
                     /locus_tag="RH057_000678"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HDV3127094.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21822..23339
                     /gene="dgt"
                     /locus_tag="RH057_000679"
     CDS             21822..23339
                     /gene="dgt"
                     /locus_tag="RH057_000679"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HDV3127095.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23469..24893
                     /gene="degP"
                     /locus_tag="RH057_000680"
     CDS             23469..24893
                     /gene="degP"
                     /locus_tag="RH057_000680"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HDV3127096.1"
                     /translation="MKKTTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKNLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25048..26205
                     /gene="cdaR"
                     /locus_tag="RH057_000681"
     CDS             25048..26205
                     /gene="cdaR"
                     /locus_tag="RH057_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
                     /protein_id="HDV3127097.1"
                     /translation="MAGWHLDTKMAQDIVARTMRIIDTNINVMDARGRIIGSGDRERI
                     GELHEGALLVLSQGRVVDIDDAVARHLHGVRQGINLPLRLEGEIVGVIGLTGEPENLR
                     KYGELVCMTAEMMLEQSRLMHLLAQDSRLREELVMNLIQAEENTPALTEWAQRLGIDL
                     NQPRVVAIVEVDSGQLGVDSAMAELQQLQNALTTPERNNLVAIVSLTEMVVLKPALNS
                     FGRWDAEDHRKRVEQLITRMKEYGQLRFRVSLGNYFTGPGSIARSYRTAKTTMVVGKQ
                     RMPESRCYFYQDLMLPVLLDSLRGDWQANELARPLARLKAMDNNGLLRRTLAAWFRHN
                     VQPLATSKALFIHRNTLEYRLNRISELTGLDLGNFDDRLLLYVALQLDEER"
     gene            complement(26294..26680)
                     /locus_tag="RH057_000682"
     CDS             complement(26294..26680)
                     /locus_tag="RH057_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HDV3127098.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(26995..27819)
                     /gene="dapD"
                     /locus_tag="RH057_000683"
     CDS             complement(26995..27819)
                     /gene="dapD"
                     /locus_tag="RH057_000683"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HDV3127099.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(27850..30522)
                     /gene="glnD"
                     /locus_tag="RH057_000684"
     CDS             complement(27850..30522)
                     /gene="glnD"
                     /locus_tag="RH057_000684"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HDV3127100.1"
                     /translation="MNTLPEQYANTALPTLPGQPQNPCVWPRDELTVGGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPIDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPEARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30584..31378)
                     /gene="map"
                     /locus_tag="RH057_000685"
     CDS             complement(30584..31378)
                     /gene="map"
                     /locus_tag="RH057_000685"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HDV3127101.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31746..32471
                     /gene="rpsB"
                     /locus_tag="RH057_000686"
     CDS             31746..32471
                     /gene="rpsB"
                     /locus_tag="RH057_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HDV3127102.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIASRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFEKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32729..33580
                     /gene="tsf"
                     /locus_tag="RH057_000687"
     CDS             32729..33580
                     /gene="tsf"
                     /locus_tag="RH057_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HDV3127103.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33727..34452
                     /gene="pyrH"
                     /locus_tag="RH057_000688"
     CDS             33727..34452
                     /gene="pyrH"
                     /locus_tag="RH057_000688"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HDV3127104.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34744..35301
                     /gene="frr"
                     /locus_tag="RH057_000689"
     CDS             34744..35301
                     /gene="frr"
                     /locus_tag="RH057_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HDV3127105.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35393..36589
                     /gene="ispC"
                     /locus_tag="RH057_000690"
     CDS             35393..36589
                     /gene="ispC"
                     /locus_tag="RH057_000690"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HDV3127106.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEDVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYDRYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANAREVARKE
                     VMRLAS"
     gene            36778..37536
                     /gene="ispU"
                     /locus_tag="RH057_000691"
                     /gene_synonym="uppS"
     CDS             36778..37536
                     /gene="ispU"
                     /locus_tag="RH057_000691"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HDV3127107.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37549..38406
                     /gene="cdsA"
                     /locus_tag="RH057_000692"
     CDS             37549..38406
                     /gene="cdsA"
                     /locus_tag="RH057_000692"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV3127108.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38418..39770
                     /gene="rseP"
                     /locus_tag="RH057_000693"
     CDS             38418..39770
                     /gene="rseP"
                     /locus_tag="RH057_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HDV3127109.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFGSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39800..42232
                     /gene="bamA"
                     /locus_tag="RH057_000694"
     CDS             39800..42232
                     /gene="bamA"
                     /locus_tag="RH057_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HDV3127110.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42354..42839
                     /gene="skp"
                     /locus_tag="RH057_000695"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42354..42839
                     /gene="skp"
                     /locus_tag="RH057_000695"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HDV3127111.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            42843..43868
                     /gene="lpxD"
                     /locus_tag="RH057_000696"
     CDS             42843..43868
                     /gene="lpxD"
                     /locus_tag="RH057_000696"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HDV3127112.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTIIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            43973..44428
                     /gene="fabZ"
                     /locus_tag="RH057_000697"
     CDS             43973..44428
                     /gene="fabZ"
                     /locus_tag="RH057_000697"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HDV3127113.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44432..45220
                     /gene="lpxA"
                     /locus_tag="RH057_000698"
     CDS             44432..45220
                     /gene="lpxA"
                     /locus_tag="RH057_000698"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HDV3127114.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45220..46368
                     /gene="lpxB"
                     /locus_tag="RH057_000699"
     CDS             45220..46368
                     /gene="lpxB"
                     /locus_tag="RH057_000699"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HDV3127115.1"
                     /translation="MTKQRPLTIALVAGETSGDILGAGLIRALKEHVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNAARDVLGIPHDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46365..46961
                     /gene="rnhB"
                     /locus_tag="RH057_000700"
     CDS             46365..46961
                     /gene="rnhB"
                     /locus_tag="RH057_000700"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HDV3127116.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEHGATEHHRRSFGPVKRALGLAS"
     gene            46998..50480
                     /gene="dnaE"
                     /locus_tag="RH057_000701"
     CDS             46998..50480
                     /gene="dnaE"
                     /locus_tag="RH057_000701"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HDV3127117.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLTLLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERVKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKPVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50493..51452
                     /gene="accA"
                     /locus_tag="RH057_000702"
     CDS             50493..51452
                     /gene="accA"
                     /locus_tag="RH057_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HDV3127118.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51551..53692
                     /gene="ldcC"
                     /locus_tag="RH057_000703"
     CDS             51551..53692
                     /gene="ldcC"
                     /locus_tag="RH057_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HDV3127119.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTDEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GTSTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVIFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVETAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECWPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLPGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53749..54138
                     /locus_tag="RH057_000704"
     CDS             53749..54138
                     /locus_tag="RH057_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV3127120.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54203..55501
                     /gene="tilS"
                     /locus_tag="RH057_000705"
     CDS             54203..55501
                     /gene="tilS"
                     /locus_tag="RH057_000705"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HDV3127121.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMGEVSEFAGTRLIRPLLARTR
                     GELAQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSAALCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRFGAFEIRRYQSQLWWINSVTGQSETIVPWQTWLQPLELPA
                     GLGSVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55550..55810)
                     /gene="rof"
                     /locus_tag="RH057_000706"
     CDS             complement(55550..55810)
                     /gene="rof"
                     /locus_tag="RH057_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HDV3127122.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55797..55997)
                     /locus_tag="RH057_000707"
     CDS             complement(55797..55997)
                     /locus_tag="RH057_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HDV3127123.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56163..56708
                     /locus_tag="RH057_000708"
     CDS             56163..56708
                     /locus_tag="RH057_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HDV3127124.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     VLFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56705..57127
                     /gene="arfB"
                     /locus_tag="RH057_000709"
     CDS             56705..57127
                     /gene="arfB"
                     /locus_tag="RH057_000709"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV3127125.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTTEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57141..57851
                     /gene="nlpE"
                     /locus_tag="RH057_000710"
                     /gene_synonym="cutF"
     CDS             57141..57851
                     /gene="nlpE"
                     /locus_tag="RH057_000710"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HDV3127126.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDALSPAQAAELKPMPQS
                     WRGVLPCADCEGIETSLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
BASE COUNT        13578 a        14372 c        16136 g        13808 t
ORIGIN      
        1 acttcacgca acgtctcccc atcaggaaac gccagctccg gtgcaatctc aaacagcggc
       61 cacagcataa atccacgatt cttcatatcg tagtgcggaa cggtcaggcg ttcggtattt
      121 atcacttcat taccaaacag catgatatcg agatccagcg tgcgtggtcc ccagcgttca
      181 gctttacgaa cacgaccctg ttgcagttct atgcgctgtg tatggttgag taactcttcc
      241 ggggcgagcg aagtttcaag cgccacggcg gcgttgaggt aatcgggttg atcttgcggc
      301 cccagcggtg gggtgcggta aaacgaagaa acggcaagaa tgtggctttc agggatatcg
      361 cctaatgctt tcagggcagc attgacctgc tccagcggag aggccagatt gctgcctatg
      421 gcaatatacg ccactgtcat gcggtaccct cacgacgcgg tgcgcgtttg cgtggacgac
      481 gagtacgacg acgcggtgac ggttcttcat ccagctcgtt aagcatccct ttttgatctg
      541 gtggtgcgga aacctggaac tcaccccacc atttcaccag acgctgcagt tcagcattac
      601 gttcaacttc agctcgcaag gccaacaggt cataagccgc acggaactta ggatgctcca
      661 gcagtttcca tgcgcgttta ccctgacgac gggacatacg caactgcaac tgccagatat
      721 cgcgggttaa cgtcgtcaga cgtttcggga ttgccagtga acggcaggct tcgtccagca
      781 cgtcgttcat cgccagcgcg aaagcgtcgt gataggtcag gccgctttcc tgggcgatct
      841 tctgtgccgt ctccagcagt gggtaccaga acatggcggc aaacaagaac gccgggttca
      901 cgcgcatatc gttatggata cgcgtatcgg tattcttcag cacctgttca atgatccgct
      961 ccatcgggct gtcgccattt tccgtgaagt agcgggtaat ggtcgggaac agcggctgga
     1021 acagatgata ttcacacaac agcttatagg tttcgtaacc gtagcccgct tgtagcagtt
     1081 taagcgattc ttcaaacagg cgtgccggtg ggatatcgtt cagcagggta gcgaggcgag
     1141 ggatcggttc tgcggtttcc gggctgatgc gcatacccaa tttggcggca aaacgtaccg
     1201 cgcgcagcat acgtaccgga tcttcacggt agcgcgtttc cgggttacca atcagacgga
     1261 taacgccgtc cttcagatcc ttcataccgc caacgtaatc acggacggta aaatccgcta
     1321 cgctgtaata gaggctgttg atagtgaaat cgcggcgctg ggcgtcttct tcgatggagc
     1381 cgaaaatgtt gtcgcgcagc aacatgccgt tttgcccgcg ttgggaggtc gtgcggtcgc
     1441 tgacgttacc ttcgtggtgt ccacggaagg ttgcaacttc gataatctcc gggccaaaca
     1501 tcacatgagc cagacggaaa cggcgaccca ccaggcggca gttacggaac agtttgcgca
     1561 cctgctcagg cgtggcgtta gtggttacgt caaaatcttt cggctttttg ccaagtaaca
     1621 ggtcgcgcac gccgccgcca accagccagg cttcgtatcc cgctttgttg agcctgtaca
     1681 ttaccttcag ggcattttca ctgatatctt tgcgggaaat agcatgctgc tcacgcggga
     1741 tcaccgtcac ctgtggacgg gcgactgcct gttcagcctc gctttcctcg cggcttagca
     1801 ccttgcggca aaaattagcg actcgggtaa aaatagtaca cctcggtagt gtcaaacatc
     1861 attcaggaca aaaaaatagc ggctaatcat agctcagcat gacgcatttg agaatgttga
     1921 atttacaatt gccgactcgg gcacggcggt aagccgccag tttttgacgg ctgactgaag
     1981 gatttgctcg acgctgaaat cctgccagtg tgcttctgcc tgctgcccca gaaattgaag
     2041 tgccgcgatt agtaccgggc gtggatcgcc tttcggcaac gcaggcgcat gattctgctt
     2101 ggaaagttta gcgccttgtg gattaagcgc cagcggcaga tgaatgtaat ctggcacttt
     2161 ccagccaaaa agctggtaca gcgagatttg ccttactgtt ggttcaatca gatcagcccc
     2221 acgcactatt tctgtaacgc cctggaaatg atcatcaacc acaacagcca ggttgtaggc
     2281 gaacaaccca tcacggcgat gaatgataaa atcttcccgt gccagttttt cgtcggcgtg
     2341 aataatgccg cgcagctggt cagtaaattg cgtgaccgga tgctgctggc ggatacgcac
     2401 tgcggcattg tctggtccat gatgcaacac ccggcaatga ccgtcgtaaa taccgccaat
     2461 gctttgaata cgcgcacgcg tacaggtgca gtaataactt agtccttgtt catgtaacca
     2521 ggcgagtgct tcacgatagg cgtggtgacg ttgcgattgc cagagaacat cgccgtccca
     2581 gtgcagaccg taatgttcca gctggcgcag gatagtttct gcggcaccgg gaacttcacg
     2641 aggcgggtcg atatcttcta tgcgtaccag ccagcgacct tgccgggcgc gagcctgcaa
     2701 atagctgccg agcgcggcga tcagagagcc aaaatgaagc tcgccggaag gagagggggc
     2761 gaagcggcca atatactgtg tgtctgtcat ctctttgaac aaaaaataag gcgggagcat
     2821 ttcccgcctg tggtaaacgt gatggaacgg ctgtaattag ccagccatct gtttttcgcg
     2881 aatttcagcc agcgttttgc agtcgatgca cagatcggct gtcgggcgcg cttccagacg
     2941 gcgaatacca atttcaacac cgcaggattc gcagtagccg aaatcttcgt cttccacttt
     3001 tttcagcgtc ttctcgatct ttttgatcag cttacgctcg cgatcgcggt tacgcagttc
     3061 gaggctgaac tcttcttcct gggctgcacg gtctaccggg tccgggaagt tggctgcttc
     3121 atcctgcata tgtgtaacgg tgcgatcgac ttcatccctg agttgattac gccatgcttc
     3181 cagaatacga cggaagtgcg ccagctgggc ttcattcata tactcttcgc ccggcttctc
     3241 ctgatatggt tccaccccag cgatggcgag aatactcagg gacgatgttt tacggttttg
     3301 cccttcttgc atgttgcttc tccttaacac gcactatcga tccccatgtt cgggggaaaa
     3361 atgaggccgc tataaatagc agatgctttt ccggatagca attatctaaa cgtaacactt
     3421 gacaactgtg tgaggaaaag cgtatttgcg cacgcgacca gaatgtaaat taaccagtta
     3481 cttactttac tacaatgtaa ccggcagtga ttttttaaga gccatgcctt cagcagaaat
     3541 ttccgctttg taagccagaa tttctacccc cctctgttga gcttctgaca atagttgcgc
     3601 gtatttctca tcgatgtggc gcgcgggtga aaaccgtgta atggctgaat gcagcacggc
     3661 gaaaaagata accgcacgct ggccttcagc cgctacgctc atcaactccc gaaggtgttt
     3721 ctgacctcgt tcagtgaccg catcgggaaa atatccctgt tcgttctccg ctaacgtaac
     3781 cgatttcact tcaatatagc agtctggacg cgaatccgcc tgcaacataa agtcaatacg
     3841 gctgcgttct gcgccgtatt ttacttcgct tttcagcgag ctatagcctg acagttctga
     3901 aattgattca ttaaggatag cctctttcgt caacctgtta gcccaaagcg tgttgacgca
     3961 aataaatgcg ccgctctggc tttgagttaa ttcccaggtg tgtgggtatt tccgtttggt
     4021 gttgtctgaa gtcgaatacc agacggtatc gccaggcgtt gcacaaccgg tcattgcacc
     4081 cgtattcggg cagtgtagcg ttaattcgcg accatcgggt gtgatcacat cggctaaaaa
     4141 acgtttgtaa cgctgaatta gcgtcgcgcg ctgtagaggg ggagagaatt ccattgcgac
     4201 aatccttgtt attgcgttag cgcccagcgt tttagcggcg tgtagcgtgt gcgtccacgg
     4261 gcaaacgagg aggcgtaaag ggtgaactcc gtcaccgcat acgaccagtt aaaacctggc
     4321 ggcgggattg tcaccgcctc tctggcgtcg cgcaataagg taatatgtgg atgaaacgga
     4381 cgattgcttt gaaaacaacc gctgcgggca gcctgtgaac ggagcatatt cgccagctgg
     4441 attaagccgc gtggcggctg acgcatccct aaccacacca cacgcgaacg cagccattgt
     4501 ccggcgtcat caagcgtgag tgtgaaacca ggttgacgaa tccgtccggc taaaagagaa
     4561 agcgccttct ctttctcggc gctcacttcg cctaaaaatg ccagagtcag atgcaaatta
     4621 tcggcggcga ccggacgtcc cgcctcaggt gggaagtgtg tggcgcgcca atggataatc
     4681 tgttcgcgga tttctgcagg taagtcgata gcaaagaaca gacgttgcgg ttcagacatg
     4741 tgaggcactc ggttatgaat taccgcgatg ctacaatgtg gcgcgaagaa tgttaaccct
     4801 ctggagcgtt ttgtgtcgtc gttgcccgtt gctgccgtct tacctgaatt acttaccgct
     4861 ctcgattgtg cgccgcaggt attattaagt gcgccgaccg gggccgggaa atcaacctgg
     4921 ctgccgctgc aactgctggc gcatcccggc attaacggga aaattatcct gctggagccg
     4981 cgtcgtctgg cggcgcgtaa cgtcgcgcaa cggctggcgg agctgcttaa cgaaaagcca
     5041 ggcgataccg ttggctaccg gatgcgtgcg caaaactgcg tcgggccgaa tacccgcctg
     5101 gaagtggtta ccgaaggcgt gctgacgcgc atgatccagc gtgacccgga actgagcggt
     5161 gttggactgg tgatccttga tgaatttcat gagcgcagct tgcaggcgga tttggcgttg
     5221 gcgctgttac tcgatgtgca acaaggtctg cgtgatgacc ttaaactgct gattatgtcg
     5281 gctaccctgg acaacgaccg cttgcagcaa atgctgccag aagcgcctgt cgtcatctca
     5341 gaagggcgct cgtttccggt tgaacgccgt tatttaccgc tgcccgcgca tcagcgtttt
     5401 gacgatgccg ttgcggtagc caccgctgaa atgctgcgtc aggaaagcgg atcattactg
     5461 ttatttttac ctggcgtcgg agaaattcag cgtgtgcagg aacaactggc ttcgcgcatc
     5521 ggcagtgatg tattgctctg cccgctgtat ggcgcgttgt cgctgaacga tcagcgaaaa
     5581 gcgatcctcc cggcaccgca agggatgcgc aaagtggtgc tggcgaccaa tattgctgaa
     5641 accagtttaa ccattgaagg tattcgtctg gtggtggatt gtgcccagga gcgtgtggcg
     5701 cgttttgatc cgcgcacggg gcttacgcga ctgattactc aacgcgttag ccaggcatcc
     5761 atgacgcagc gtgccgggcg cgccgggcgt ctggagccgg gtatctgcct gcatttaatc
     5821 gccaaggaac aagcagaacg cgccgcggcg caaagtgaac cggaaatctt acaaagcgat
     5881 ctttccggtt tgctgatgga attactgcaa tggggatgca gcgatccggc gcagatgagc
     5941 tggctggatc aaccgccaac ggtgaatcta ctggccgcga aacgcctgtt acaaatgctg
     6001 ggggcgctgg agggggaacg acttagtgcg caggggcaaa aaatggcagc gctgggtaac
     6061 gatccgcgtt tagcggcaat gctggttagc gcgaagaacg acgacgaagc tgctaccgcg
     6121 gcaaaaattg ccgccattct cgaagagccg ccacggatgg gcaatagtga cctgggcgtg
     6181 gcgttttcgc gcaatcaacc agcctggcag caacgtagtc agcaactgtt aaaacgctta
     6241 aacgtacgtg gcggtgaggc agacagttcg cttatcgcgc cgctacttgc cggggcgttt
     6301 gccgatcgca ttgctcgtcg ccgtgggcaa gatggacgct atcaactggc aaacggcatg
     6361 ggagcgatgc tcgatgccaa cgacgcgcta agccgccacg aatggttgat cgcaccgtta
     6421 ttattgcagg gcagcgcctc gccggatgcg cggattttac tggcgctgct ggtcgatatt
     6481 gatgagttag tacaacgctg cccgcagctg gtacagcagt ctgacactgt ggagtgggat
     6541 gacgcgcaag gtacgctgaa agcctggcgt cggctacaaa tcggtcagtt gacggtgaaa
     6601 gtgcagccgc tggcgaaacc gtcagaagac gagttgcatc aggcgatgct taatggcatc
     6661 cgtgataaag gtttaagcgt gctcaactgg acggcggaag cggaacagct acgcttgcgt
     6721 ttgttatgcg ccgcaaagtg gttgccggaa tatgactggc cagcggttga tgatgaaagt
     6781 ttattggcag cgctggaaac gtggctgctg ccacatatga ctggcgtaca ttcactacgc
     6841 ggcctgaaat cactcgacat ttatcaggca ctacgcggat tacttgattg gggaatgcag
     6901 caacgtctgg atagtgaatt gcctgcgcat tacactgtgc cgacgggaag ccggatcgcc
     6961 attcgttatc atgaagataa cccgcccgcg ctggcggtga gaatgcaaga gatgtttggc
     7021 gaggccacca atccgacgat cgcccagggg cgcgtgccgc tggtgctgga gttgctttca
     7081 cctgcccaaa ggccattaca aatcacacga gatttgagcg ccttctggaa aggagcgtac
     7141 cgtgaggtgc aaaaagagat gaaagggcgt tatcccaaac atgtctggcc ggacgacccg
     7201 gcaaatactg caccgacgcg acggacgaaa aagtattcgt aaggttggtt ttctccctct
     7261 ccctgtggga gagggccggg gtgagggcat cagcgcgcac gttcaccctc accctaaccc
     7321 tctccctcaa gggagagggg accgatcgag cacaaatttt gagagatatc ttcttctgtc
     7381 ttgtaacaga agaacagaaa atcgggcttt tgcgcctgaa tattgcggag aaaaagcatg
     7441 gccgggaatg accgcgagcc aattggacgc aaagggaaac cgacgcgtcc ggtcaaacaa
     7501 aaggtaagcc gtcgtcgtta cgaagatgac gatgattacg acgattatga tgactatgag
     7561 gatgaagaac cgatgccgcg caaaggtaag ggcaaaggca aagggcgtaa gcctcgtagc
     7621 aaacgcggct ggctatggct actgctaaaa ctggctatcg tttttgccgt gctgatcgcc
     7681 atttacggcg tttatctcga tcaaaaaatt cgtagccgta ttgatggcaa ggtctggcaa
     7741 ctgcctgcgg cagtttatgg ccgaatggtc aatcttgagc cagatatgac catcagcaag
     7801 aacgagatgg tgaagctgct ggaggcgact cagtatcgtc aggtgtcgaa aatgacccgt
     7861 cctggcgaat ttaccgtgca ggccaacagc attgagatga tccgccgtcc gtttgatttc
     7921 ccggacagta aagaagggca ggtgcgtgcg cgtctgacct ttgatggtga tcatctggcg
     7981 acgatcgtca atatggagaa caaccgtcag ttcggtttct tccgtcttga tccgcgcctg
     8041 atcaccatga tctcttcgcc aaacggtgag cagcgtctgt ttgtgccgcg cagtggtttc
     8101 cctgatttgc tggtggatac tttgctggcg acagaagacc gccatttcta cgagcatgat
     8161 gggatcagcc tctactcaat tgggcgtgcg gtgctggcaa acctgaccgc aggtcgcacg
     8221 gtgcagggtg cgagtacgct gacgcaacag ctggtgaaaa acctgttcct ctccagcgag
     8281 cgctcttact ggcgtaaagc gaacgaagcc tacatggcgc tgatcatgga cgcgcgttac
     8341 agcaaagacc gtattcttga gctgtatatg aacgaggtgt atctcggtca gagcggcgac
     8401 aacgaaatcc gcggcttccc gctggcaagc ttgtattact ttggtcgccc ggtagaagag
     8461 ctaagcctcg accagcaggc gctgttagtc ggtatggtga aaggggcgtc catctacaac
     8521 ccgtggcgta acccaaaact ggcgctggag cgacgtaatc tggtgctgcg tctgctgcaa
     8581 cagcaacaga ttattgatca agaactctat gacatgttga gtgcccgtcc gctgggggtt
     8641 cagccgcgcg gtggggtgat ctctcctcag ccagccttta tgcaactggt gcgtcaggag
     8701 ctgcaggcaa aactgggcga taaggtaaaa gatctctccg gcgtgaagat cttcactacc
     8761 tttgactcgg tggcccagga cgcggcagaa aaagccgccg tggaaggcat tccggcactg
     8821 aagaaacagc gtaagttgag cgatcttgaa actgcgattg tggtcgtcga ccgctttagt
     8881 ggtgaagttc gtgcgatggt cggaggttct gagccgcagt ttgcgggcta caaccgtgcg
     8941 atgcaggcgc gtcgttcgat tggttccctt gcaaaaccag cgacttatct gacggcttta
     9001 agccagccga aaatctatcg cctgaacacg tggattgccg atgcgccaat cgcgctgcgt
     9061 cagccgaatg gccaggtctg gtcaccgcag aacgatgacc gtcgttatag cgaaagcggc
     9121 agagtgatgc tggtggatgc gttgacccgt tcgatgaacg tgccgacggt aaatctgggg
     9181 atggcgctgg ggctgcctgc agttacggat acctggatca aactgggcgt accgaaagat
     9241 cagctgcatc cggttccggc gatgctgctg ggggcgttga acttaacgcc aatcgaagtg
     9301 gcgcaggcgt tccagaccat cgccagcggt ggtaaccgtg caccgctttc tgcactgcgt
     9361 tcggtaatcg cggaagatgg caaggtgctg tatcagagct tcccgcaggc agaacgcgct
     9421 gttccggcgc aggcggcgta tctgacacta tggaccatgc agcaggtggt acaacgtggt
     9481 acgggtcgtc agcttggggc gaaatacccg aacctgcatc tggcagggaa aacagggact
     9541 accaacaata acgtagatac ctggtttgca ggcattgacg gcagcacggt gaccatcacc
     9601 tgggtaggcc gtgataacaa ccagccgacc aaactgtatg gagccagcgg ggcaatgtcg
     9661 atttatcagc gttatctggc taaccagacg ccaacgccgc tgaatcttgt tccgccagaa
     9721 gatattgcag atatgggcgt ggactacgac ggcaactttg tttgcagcgg tggcatgcgt
     9781 gtcttgccgg tctggaccag cgatccgcaa tcgctgtgcc agcagagcga gatgcagcag
     9841 cagccgtcag gcaatccgtt tgatcagtct tctcagccgc agcaacagcc gcaacagcaa
     9901 cctgctcagc aagagcagaa agacagcgac ggtgtagccg gttggatcaa ggatatgttt
     9961 ggtagtaatt aacatctaag cgtgaaatac cggatggcga gttgccatcc ggtaaaataa
    10021 catcccatct aagatattaa ccctttcttt tcatctggtt gtttattaac ccttcaggaa
    10081 cgctcagatt gcgtaccgct tgcgaacccg ccagcgtttc gaatattatc ttatctttat
    10141 aataatcatt ctcgtttacg ttatcattca ctttacatca gagatatacc aatggcgcgt
    10201 tccaaaactg ctcagccaaa acactcactg cgtaaaatcg cagttgtagt agccacagcg
    10261 gtaagcggca tgtctgttta tgcacaggca gcggttgaac cgaaagaaga cactatcacc
    10321 gttaccgctg cacctgcgcc gcaagaaagc gcatgggggc ctgctgcaac tattgcggcg
    10381 cgacagtctg ctaccggcac taaaaccgat acgccgattc aaaaagtgcc acagtctatt
    10441 tctgttgtga ccgccgaaga gatggcgctg catcagccga agtcggtaaa agaagcgctt
    10501 agctacacgc cgggtgtctc tgttggtacg cgtggcgcat ccaacaccta tgaccacctg
    10561 atcattcgcg gctttgcggc agaaggccaa agccagaata actatctgaa tggcctgaag
    10621 ttgcagggca acttctataa cgatgcggtc attgacccgt atatgctgga acgcgctgaa
    10681 attatgcgtg gcccggtttc cgtgctttac ggtaaaagca gtcctggcgg cctgttgaat
    10741 atggtcagca agcgtccgac caccgaaccg ctgaaagaag ttcagtttaa agccggtact
    10801 gacagcctgt tccagactgg ttttgacttt agcgattcgt tggatgatga cggtgtttac
    10861 tcttatcgcc tgaccggtct tgcgcgttct gccaatgccc agcagaaagg gtcagaagag
    10921 cagcgttatg ctattgcacc ggcgttcacc tggcgtccgg atgataaaac caattttacc
    10981 ttcctttctt acttccagaa cgagccggaa accggttatt acggctggtt gccgaaagag
    11041 ggaaccgttg agccgctgcc gaacggtaag cgtctgccga cagactttaa tgaaggggcg
    11101 aagaacaaca cctattctcg taatgagaag atggtcggct acagcttcga tcacgaattt
    11161 aacgacacct ttactgtgcg tcagaacctg cgctttgctg aaaacaaaac ctcgcaaaac
    11221 agcgtttatg gttacggcgt ctgctccgat ccggcgaatg cttacagcaa acagtgtgcg
    11281 gcattagcgc cagcggataa aggccattat ctggcacgta aatacgtcgt tgatgatgag
    11341 aagctgcaaa acttctccgt tgatacccag ttgcagagca agtttgccac tggcgatatc
    11401 gaccacaccc tgctgaccgg tgtcgacttt atgcgtatgc gtaatgacat caacgcctgg
    11461 tttggttacg acgactctgt gccactgctc aatctgtaca atccggtgaa taccgatttc
    11521 gacttcaatg ccaaagatcc ggcaaactcc ggcccttacc gcattctgaa taagcagaaa
    11581 caaacgggcg tttatgttca ggatcaggcg cagtgggata aagtgctggt caccctgggc
    11641 ggtcgttatg actgggcaga tcaagaatct cttaaccgcg ttgctgggac gaccgataaa
    11701 cgtgatgaca aacagtttac ctggcgtggt ggtgttaact acctgtttga taatggtgta
    11761 acaccttact tcagctatag cgaatcgttt gaaccttctt cgcaagttgg gaaggatggt
    11821 aatattttcg caccgtctaa aggtaagcag tatgaagtcg gcgtgaaata tgtaccggaa
    11881 gatcgtccga ttgtagttac tggtgccgtg tataatctca ctaaaaccaa caacctgatg
    11941 gcggaccctg agggttcctt cttctcggtt gaaggtggcg agatccgcgc acgtggcgta
    12001 gaaatcgaag cgaaagcggc gctgtcggcg agtgttaacg tagtcggttc ttatacttac
    12061 accgatgcgg aatacaccac cgatactacc tataaaggca atacgcctgc acaggtgcca
    12121 aaacacatgg cttcgctgtg ggctgactat accttctttg acggtccgct ttcaggtctg
    12181 acgctgggca ccggtggtcg ttatactggc tccagctatg gtgatccggc taactccttt
    12241 aaagtgggaa gttatacggt cgtggatgcg ttagtgcgtt atgatctggc gcgagtcggc
    12301 atggcgggct ccaacgtggc gctgcatgtt aacaacctgt tcgatcgtga atacgtcgcc
    12361 agctgcttta acacttatgg ctgcttctgg ggcgcagaac gtcaggtcgt tgcaaccgca
    12421 accttccgtt tctaatttct cttttggggc acggattacc gtgcccattt cacaagttgg
    12481 ctgttatgca ggaatacacg aatcattccg ataccacttt tgcactgcgt aatatctcct
    12541 ttcgtgtgcc cgggcgcacg cttttgcatc cgctgtcgtt aacctttcct gccgggaaag
    12601 tgaccggtct gatcggtcac aacggttctg gtaaatccac tctgctcaaa atgcttggcc
    12661 gtcatcagcc tccgacggaa ggggagattc ttcttgatac ccagccgctg gaaagctgga
    12721 gcagcaaagc gttcgcccgc aaagtggctt atttgccgca gcagcttcct ccggcagaag
    12781 ggttgaccgt gcgtgaactg gtggcgattg gtcgttaccc gtggcatggc gcgctggggc
    12841 gctttggggc ggcagatcgc gaaaaagtcg aggaagctat ctcgctggtt ggcttaaaac
    12901 cgctggcgca tcggctggtc gatagtctct ctggcggcga acgtcaacgg gcgtggatcg
    12961 ccatgctggt ggcgcaggat agccgttgtc tgttgctcga cgaaccgact tcggcgctgg
    13021 atatcgccca ccaggttgat gtgctggcgc tggtgcaccg tttaagtcag gaacgtggcc
    13081 tgacggtcat tgccgtgctg cacgatatca atatggcggc acgctactgt gattatctgg
    13141 tcgccctgcg cggtggtgaa atgattgctc agggaacgcc tgcggaaatt atgcgcggcg
    13201 aaaccctcga aatgatttat ggcatcccga tgggtatttt gccgcatccg gcgggtgctg
    13261 cacctgtgag ttttgtttat tgatgagcgg cttacctctt atttcgcgcc gtcgactgtt
    13321 aacggcgatg gcgctttctc cgttgttatg gcagatgaat accgcccacg cggcggctat
    13381 tgaccccaat cgtattgtgg cgctggagtg gttgccggtg gaattactgc tggcgctcgg
    13441 catcgtgcct tacggcgtgg cggataccat caactatcgc ctgtgggtca gcgaaccacc
    13501 attgccggac tcagtgattg acgtcggttt gcgtacagaa cctaaccttg aactgctgac
    13561 cgaaatgaaa ccgtcgttta tggtctggtc ggcaggatat ggcccttcac cagaaatgct
    13621 ggctcgtatt gcgccggggc gtggatttaa cttcagcgac ggcaaacagc cgctggcgat
    13681 ggcgcgtaag tcactgacgg aaatggcaga tttacttaac ctgcaaagcg cagcagaaac
    13741 gcatttagcg cactatgaag actttatccg cagcatgaaa ccccgctttg tgaagcgtgg
    13801 tgaacgcccg ttattgctga cgacgcttat cgatccgcgc catatgctgg ttttcggtcc
    13861 aaacagcttg tttcaggaaa ttcttgatga gtacggcatc ccaaatgcct ggcaagggga
    13921 aaccaacttc tggggcagta ccgccgtcag catcgatcgt ctggcggcgt ataaagatgt
    13981 tgatgtgctc tgttttgatc acgacaacag caaagacatg gacgcgctaa tggcaacccc
    14041 gctgtggcag gccatgccgt ttgtccgcgc cggacgcttt cagcgcgtac ccgcagtctg
    14101 gttttatggt gcgacgctct cggcaatgca ctttgtgcgc gttctggata acgccatcgg
    14161 aggtaaagcg tgagtaaacg aattgcgctt tttccggcgt tattgctggc gctgctggtg
    14221 attgtcgcta cggcgctgac ctggatgaac ttttcgcagg cgctgccgcg tagccagtgg
    14281 gcgcaggccg cctggtcgcc ggatattaac gtcatcgagc agatgatttt tcactacagc
    14341 ttgttaccac gtctggcgat ttctctactg gtgggcgcgg gcctggggct ggtgggcgtg
    14401 ctgtttcagc aagtgctgcg taacccgctg gcggaaccga cgacgttggg cgttgctaca
    14461 ggcgcgcaac tggggattac cgtcaccacg ctctgggcga tcccaggtgc tatggcgagc
    14521 cagtttgctg cgctggcagg ggcttgcgtt gttggcttaa ttgtctttgg cgtcgcgtgg
    14581 gggaaacggc tgtcgccggt aacgctgatt ctcgcggggc tggtagtgag cctttattgt
    14641 ggggcaatca atcagttact ggttatcttc catcatgacc agttgcaaag catgttcctg
    14701 tggagcaccg gaacgctgac gcaaaccgac tggggcggcg ttgagcgttt atggccgcag
    14761 ttgctgggcg gcgtgatgct gacgttactg ctacttcgtc cgttaaccct gatggggctt
    14821 gatgatggcg tggcgcgcaa tctcgggctg gccttgtcgc tcgcgcgtct ggcggcgctg
    14881 tcgctggcga tagtcatcag tgcgctgctg gtgaacgcag tggggattat cggctttatc
    14941 gggttgttcg cgccactgct ggcaaaaatg ctgggggcgc ggcgtttgtt gccacggttg
    15001 atgctggcgt cgctgattgg tgcgttaatt ctgtggcttt ctgatcaaat catcctctgg
    15061 ctgactcgcg tgtggatgga agtttccacc ggttcggtca ctgcgttgat cggtgcgccg
    15121 ctgctactgt ggctgctgcc gcgtttacgc agcattagcg cgccggatat gaaggtcaac
    15181 gatcgtgtcg cgactgaacg ccaacatgtg ctggcgtttg ccctcgcggg cggcgtgctg
    15241 ctgttgatgg ctgtggtagt ggcgctgtcg tttggtcgtg atgcgcacgg ctggacgtgg
    15301 gcgagcgggg cgttgctcga ggatttaatg ccctggcgct ggccgcgaat tatggcggcg
    15361 ctgtttgcgg gcgtcatgct ggcggtagcg ggctgtatta ttcagcggct gaccggcaac
    15421 ccgatggcaa gcccggaagt gctggggatt agctccggcg cagcgtttgg cgtggtgttg
    15481 atgctgtttc tggtgccggg taatgccttt ggctggctgt tacctgcagg cagtctcggg
    15541 gcggcggtga cgctgttgat cattatgatc gccgccggac gcggtggatt ttccccacac
    15601 cgcatgttac tggcggggat ggcgttaagt accgcgttca ccatgctttt gatgatgttg
    15661 caggcaagtg gtgacccgcg aatggcgcaa gtgctgacct ggatttccgg ttcgacctac
    15721 aacgcgaccg atgcgcaggt ctggcgcacg ggaattgtga tggtaatttt gctggcgatt
    15781 accccgctgt gccgccgctg gctgaccatt ttaccgctgg gtggtgatac cgcccgagcc
    15841 gtaggaatgg cgctgacgcc gacgcgaatt gcgctgctgc tgttagcggc ttgcctgacg
    15901 gcgaccgcga cgatgactat tggaccgttg agttttgttg gtttaatggc accgcatatt
    15961 gcgcggatga tgggctttcg acggacgatg ccacacatcg taatttcggc gctggtgggt
    16021 ggtttactgc tggtgttcgc tgactggtgt gggcggatgg tgctgtttcc attccagatc
    16081 ccggcggggc tgctgtcaac ctttatcggc gcgccatatt ttatctattt gttgagaaag
    16141 cagagccgtt aagagaatgc ctcgcccggt gggcgaggca aaatcatcac aacttcgcaa
    16201 acacccgacg tgcagcatcg atggtgttat tgatatcttc catgctgtgc gccacggaca
    16261 taaagcccgc ttcaaacgct gacggtgcca ggtaaacacc ttcgtccagc atcatatgga
    16321 agaaacgctt aaagcgttcc acgtcacagg ccatcacatc ctgatagcac gtcacggact
    16381 cggcgtcggt aaagaaaata ccgaacatgc cgccaacgtg gttaacgacc agcggaattc
    16441 cggcttcttt tgccgcttcc agcagacctt ctgccagacg tgttgtcagc tcatccagcg
    16501 tttcgtgaac gcccggctgc gcgacttcat tcagacaggc gaaacccgct gccatcgcaa
    16561 tcgggttacc ggaaagcgta cccgcctgat agaccggacc cgtcggggcc agcgcatcca
    16621 ttacatcacg acgaccaccg aatgcgccta ccggcattcc accgccgatg attttgccga
    16681 ggcaggttaa atctggcact acgccgtaat aatcctgtgc accagccagt gccacgcgga
    16741 agccagtcat tacttcatcg atgatcagca atgcgccaaa ttcgtcgcac agcgcgcgca
    16801 gacctggcag gaactctggc agcggcggaa cacagttcat attgcctgcc accggctcaa
    16861 cgataataca ggcaatctct tgcgggtatt gctcaaacgc ggcgcgtaca gaagccagat
    16921 cgttataagt acaggttaag gtatgtttgg cgaaatctgc cggaacaccc ggcgagtttg
    16981 gctggcctaa cgtgagtgcg ccagaaccgg ctttcaccag caggcagtca gcgtgaccgt
    17041 ggtaacaacc ttcaaattta atgattttgt cgcgaccggt aaaaccacgg gccaggcgga
    17101 tggcgctcat ggtcgcttca gtgccggagt tcaccatgcg caccatatcc atggtcggga
    17161 ccagttcggt caccagttgc gccattttca cttccatttc ggttggtgca ccaaagctta
    17221 aaccacgctc ggcggcttca atcacggcat tgcggattgc cggatggtta tggcccagca
    17281 ccatcggccc ccaggaaccg acataatcga tataggcttt gccatcaaca tcgtacagat
    17341 aagcgccgtc cgctttttcg ataaacagtg gagtgccgcc cacgccagta aaggcgcgaa
    17401 caggggagtt cacaccgcca gggatcagct cgcgcgctgc gctgtaaaga ttttcagact
    17461 tactcatgga gggttcctga ttcgtagaaa aagtgaatgg ctgctattct atgttattca
    17521 taacaagtta aatactcgtc aaacatcagg ctgcttgtac tggtgcaatc ctgatttctt
    17581 agagtataaa agttttgtgc atttgaaaca ttacgctttg caaaggattt tcatggaacg
    17641 tgcgagtaaa atgccgtcat cttatttgta tgaccaataa gtgatcattg gatgaaaact
    17701 gatactccct ctttagaaac accgcaggcc gcgcgcctgc gacgcagaca actgattcgc
    17761 caacttcttg agcgcgataa aaccccgtta gccattttgt ttatggcggc agtcgtcggc
    17821 acgcttgtcg ggctggcagc ggttgctttt gacaaaggtg tcgcctggtt gcagaaccaa
    17881 cgtatggggg cgctggtaca tactgctgat aattatccgc tactgttaac cgtcgctttt
    17941 ctctgttcgg cggtgctggc gatgttcggc tactttttgg tgcgcaaata cgcgcccgaa
    18001 gcagggggat cggggatccc ggaaattgaa ggggcgctgg aagatcaacg tcctgttcgc
    18061 tggtggcgtg tattgccggt gaaattcttt ggcgggctgg ggacactcgg cggaggcatg
    18121 gtgctagggc gcgaaggacc aaccgtgcag atcggcggca atatcggtcg catggtgctt
    18181 gatattttcc gcctgaaagg tgatgaagct cgccatacgc tgctggcaac cggtgctgct
    18241 gcggggctgg ctgcggcctt taacgcgccg ctggcgggta ttttgtttat tatcgaagag
    18301 atgcgtccgc agtttcgcta tacgttaatt tcgattaaag cggtatttat tggcgtcatt
    18361 atgtcgacca ttatgtaccg gatttttaat catgaagttg cgttgattga cgtcggtaaa
    18421 ctttctgatg cgccgcttaa tacgctgtgg ctttatctga tcctcggtat tatttttggc
    18481 attttcggcc ctatttttaa taaatgggtg ctggggatgc aggatttgct gcaccgtgtg
    18541 cacggcggca atattaccaa atgggtactg atgggtggtg cgattggcgg tctgtgtgga
    18601 ttgctggggt ttgtggcacc agcaacgtcg ggcggcggtt ttaacctgat tcctatcgct
    18661 accgcgggga atttcagcat gggaatgctg gtgtttatct tcgtcgctcg ggtcattacc
    18721 accttactct gcttctcttc cggcgcgccg ggcggtattt ttgccccgat gctggcgctg
    18781 ggtactgtgc tgggaaccgc tttcggaatg gttgccgttg agctgtttcc gcaatatcac
    18841 ctggaggcgg ggacgtttgc tattgccgga atgggggcat tactggcggc atctattcgc
    18901 gcgccgttaa cggggatcat tctggttctg gagatgaccg ataactacca gctcattttg
    18961 ccaatgatta ttaccggtct tggcgcaaca ctattagcgc aatttaccgg cgggaaaccg
    19021 ctatactcgg cgattcttgc gcgcacgctg gcaaaacagg aagctgagca actggcgcga
    19081 agcaaggctg catcagccag cgagaatact tgaacgaaat accagggtat tagataatgg
    19141 cgattattat tgggttagaa tttgcccaat tgccgatgtc gtttggagca aaatatgagt
    19201 gatgacgtag cactgccgct ggagtttacc gacgcagcag ccaacaaagt taaaagcctg
    19261 atcgctgacg aagataaccc gaatctgaaa ttacgcgtgt atatcaccgg tggcggttgc
    19321 agcggcttcc agtatggttt cacctttgat gatcaggtga acgaaggcga tatgaccatc
    19381 gaaaaacagg gcgttggcct ggtggttgat ccgatgagcc tgcaatatct ggtcggcggt
    19441 tccgttgact acaccgaagg actggaaggt tctcgtttca tcgtgaccaa cccgaacgcg
    19501 aaaagcacct gcggttgcgg ttcttccttt agcatctaat ctgttgttca tgtgccggat
    19561 aaactatccg gcacatcttc tctcttcaac gcccattctc atccagcgca aacgtcggta
    19621 gcttaagatg ccaacgaata gccgccagcc gaatcaatag cgtcacgacc atgcccatca
    19681 tactggctgt ttccagtggt acggaaaatg tgtaataagc cgtagcgtgg acaataccgc
    19741 cgataataca ggcagttgcg tagatttctg tacgtaaaat catggggatc tcgcgggcca
    19801 gtacatcacg aatgatcccg ccgccaacgc cagtaatgac gcccatacaa accgcgatta
    19861 acggaccggc ttccgcatta aaggctttat tcacgccaat gccgacaaac accgccagac
    19921 caacggcgtc cagcaccggc aacatccatt ttggtaagcg tcttggctgg cgcaccagca
    19981 cgatggtcag catgctggtg accattgcaa cgaccagatc ggtgggatct ttcacccaaa
    20041 ataccgggcc gtgatccagc gccatgtcgc gaattgttcc gccgcctact gcggtaacca
    20101 cgcccagtac cagaacacca aaagggtcca tacgcaattt tccggctaac aaaacgccgg
    20161 agatggcaaa taccgctgtg ccgactatat ccagccaata gacgagcatt gttcaatccc
    20221 cactgagcac ctgaaaaggt caggcactaa tctacctgtg aaagcgcatt acagagctgt
    20281 tgtgcagcga ggataatacg tgggcttgca cgttcaaacc agtcactcgt gagaggaata
    20341 acgggaattt tgagctgttc accccagtat tgtttgattt taggaatttg gtccggtccg
    20401 cctgtaatga caatcgcctg tggcgagcgt gctaacacct gttcgcggct aacttgcggc
    20461 cagggaaccc ggctgtcttt aaagatgttt tctccgccac aaacttcgag tacctggttc
    20521 tgaatcgact cttttccact ggtaaatggc ggattaatgc cgaattgcag aaaaacacgt
    20581 tttttaggtt tatcagcata ttgcgctttc aattgcgcgt actgatccag cagggattgc
    20641 gcggcttgtt cggccttgtc tggttgcgga ctccaggggg ccagttgacg taacgcattg
    20701 gcaatttgtt caatgcttgt cgcatcgacc cacatcactt ttattcccag cgaagccagc
    20761 tggtcaacct gccgctcggc attacctcca cgccaggcaa tcaccagatc gggtttcagc
    20821 gcgacaatgc gttccagatt catcccctgc caggtggaaa cctgctcaat cttttgcgct
    20881 tgtggaggat agtcggaata gctgctgacc ccaaccggcg tgatcccggc ggcaaaggca
    20941 agttcagtgt tggcgggaga aagcgtgatg acgcgcggcg cggcgttgag ccacagtggc
    21001 gcaagaaaag acagggcgac cagcgccctg aacagtgact tagccatgtg caagtttctg
    21061 caccagtgac tcaaccatca ggctggactg tttagcggca acagccagga actcatcgaa
    21121 gctaagatga gactgttgat cggccacgtc ggagatggcg cgtacgacaa caaacgggac
    21181 gttgaaattg tggcagacat gggcgattgc cgtcgcttcc atctctacag caatggcctg
    21241 tgggaagttg tggcggattt tcgccagacc aacagaaccg ttgatgaaag cgtcgccgct
    21301 aacgatcagg ccacgtacag cgttaagatt cagttcggca atgcaggcct cagcggcagc
    21361 gatcagttta tcgtcagctt taaagcctgc cggacagcct ggtaactgac cgtattcata
    21421 accaaatgcc gtgacatccg cgtcgtgata acgtgcttcg tccgagacaa cgatatcgcc
    21481 cactttcagc gttggtgcca ggccaccggc agaaccggtg ttaataatca catctggctt
    21541 gcagtgttcc aacagcaaag tggcacccag cgccgcagcg actttaccga tgcccgattt
    21601 cagaagcgca acctcggttc cattcagttg gccggtatag atttcgcaac cgcccagact
    21661 gatagtttga cggttttcga ttttgtcacg cagcagcgta acttcttctt ccattgcacc
    21721 aatgatgccg attttcatag atttactcgc gataagcccg atttgaaggc atagtttacc
    21781 atgcgcttac ggggaagcgt atttctcacg cgggagagga catggcacag attgatttcc
    21841 gaaaaaaaat aaactggcat cgtcgttacc gttcaccgca gggcgttaaa accgaacatg
    21901 agatcctgcg gatcttcgag agcgatcgcg ggcgtatcat caactctccg gcaattcgtc
    21961 gtctgcaaca aaagacgcag gtttttccac tggagcgcaa tgccgccgtg cgcacgcgtc
    22021 ttacccactc gatggaagtc cagcaggtgg ggcgctacat cgccaaagaa attttaagcc
    22081 gtctgaaaga gcttaaattg ctggaagcat acggcctgga tgaactgacc ggaccttttg
    22141 aaagcattgt tgagatgtca tgcctgatgc acgatatcgg caatccgccg tttggtcatt
    22201 ttggcgaagc agcgataaat gactggtttc gccaacgttt gcacccggaa gatgccgaaa
    22261 gccagcctct gactgacgat cgctgcagcg tggcggcact acgtttacgg gacggggaag
    22321 aaccgcttaa cgagctgcgg cgcaagattc gtcaggactt atgtcatttt gaggggaatg
    22381 cacaaggcat tcgtctggtg catacattga tgcggatgaa tctcacctgg gcacaggttg
    22441 gcggtatttt aaaatatacc cgtccggcgt ggtggcgtgg cgaaacgcct gagacacatc
    22501 actatttaat gaaaaagccg ggttattatc tttctgaaga agcctatatt gcccggttgc
    22561 gtaaagaact taatttggcg ctttacagtc gttttccatt aacgtggatt atggaagctg
    22621 ccgacgatat ctcctattgt gtggcagacc ttgaagatgc agtagagaaa agaatattta
    22681 ccgttgagca gctttatcat catttgcacg aagcgtgggg ccagcatgag aaaggttcgc
    22741 tcttttcgct ggtggttgaa aatgcctggg aaaaatcacg ctcaaatagt ttaagccgca
    22801 gtacggaaga tcagtttttt atgtatttac gggtaaacac cctaaataaa ctggtaccct
    22861 acgcggcaca acgatttatt gataatctgc ctgcgatttt cgccggaacg tttaatcatg
    22921 cattattgga agatgccagc gaatgcagcg atcttcttaa gctatataaa aatgtcgctg
    22981 taaaacatgt gtttagccat ccagatgtcg agcagcttga attgcagggc tatcgggtca
    23041 ttagcggatt attagagatt tatcgtcctt tattaagcct gtcgttatca gactttactg
    23101 aactggtaga aaaagaacgg gtgaaacgtt tccctattga atcgcgctta ttccacaaac
    23161 tctcgacgcg ccatcggctg gcctatgtcg aggctgtcag taaattaccg tcagattctc
    23221 ctgagtttcc gctatgggaa tattattacc gttgccgcct gctgcaggat tatatcagcg
    23281 gtatgaccga cctctatgcg tgggatgaat accgacgtct gatggccgta gaacaataac
    23341 caggcttttg taaagacgaa caataaattt ttaccttttg cagaaacttt agttcggaac
    23401 ttcgcgctat aaaacgaatc tgaagaacac agcaattttg cgttatctgt taatcgagac
    23461 tgaaatacat gaaaaaaacc acattagcac tgagtgcact ggctctgagt ttaggtttgg
    23521 cgttatctcc gctctctgca acggcggctg agacttcttc agcaacgaca gcccagcaga
    23581 tgccaagcct tgcaccgatg ctcgaaaagg tgatgccttc agtggtcagc attaacgtag
    23641 aaggtagcac aaccgttaat acgccgcgta tgccgcgtaa tttccagcag ttcttcggtg
    23701 atgattctcc gttctgccag gaaggttctc cgttccagag ctctccgttc tgccagggtg
    23761 gccagggcgg taatggtggc ggccagcaac agaaattcat ggcgctgggt tccggcgtca
    23821 tcattgatgc cgataaaggc tatgtcgtca ccaacaacca cgttgttgat aacgcgacgg
    23881 tcattaaagt tcaactgagc gatggccgta agttcgacgc gaagatggtt ggcaaagatc
    23941 cgcgctctga tatcgcgctg atccaaatcc agaacccgaa aaacctgacc gcaattaaga
    24001 tggcggattc tgatgcactg cgcgtgggtg attacaccgt agcgattggt aacccgtttg
    24061 gtctgggcga gacggtaact tccgggattg tctctgcgct ggggcgtagc ggcctgaatg
    24121 ccgaaaacta cgaaaacttc atccagaccg atgcagcgat caaccgtggt aactccggtg
    24181 gtgcgctggt taacctgaac ggtgaactga tcggtatcaa caccgcgatc ctcgcaccgg
    24241 acggcggcaa catcggtatc ggttttgcta tcccgagcaa catggtgaaa aacctgacct
    24301 cgcagatggt ggaatacggc caggtgaaac gcggtgagct gggtattatg gggactgagc
    24361 tgaactccga actggccaaa gcgatgaaag ttgacgccca gcgcggtgct ttcgtaagcc
    24421 aggttctgcc taattcctcc gctgcaaaag cgggcattaa agcgggtgat gtgatcacct
    24481 cactgaacgg taagccgatc agcagctttg ccgcactgcg tgctcaggtg ggtactatgc
    24541 cggtgggcag taaactgacc ctgggcttac tgcgcgacgg taagcaggtt aacgtgaacc
    24601 tggaactgca gcagagcagc cagaatcagg ttgattccag ctccatcttc aacggcattg
    24661 aaggcgctga gatgagcaac aaaggcaaag atcagggcgt ggtagtgaac aacgtgaaaa
    24721 cgggcactcc ggctgcgcag atcggcctga agaaaggtga tgtgattatt ggcgcgaacc
    24781 agcaggcagt gaaaaacatc gctgaactgc gtaaagttct cgacagcaaa ccgtctgtgc
    24841 tggcactgaa cattcagcgc ggcgacagca ccatctacct gttaatgcag taatctccct
    24901 caaccccttc ctgaaaacgg gaaggggttc tccttacaat ctgtgaactt caccacaact
    24961 ccatacatct tcatcatcct ttaggcattt gcacaatgcc gtacgttacg tacttcctta
    25021 tgctaagccg tgcataacgg aggacttatg gctggctggc atcttgatac caaaatggcg
    25081 caggatatcg tggcacgtac catgcgcatc atcgatacca atatcaacgt aatggatgcc
    25141 cgtgggcgaa ttatcggcag cggcgatcgt gagcgtattg gtgaattgca cgaaggtgca
    25201 ttgctggtac tttcacaggg acgagtcgtc gatatcgatg acgcggtggc acgtcatctg
    25261 cacggtgtgc ggcaagggat taatctaccg ttacggctgg aaggtgaaat tgtcggcgta
    25321 attggcctga caggtgaacc agagaatctg cgtaaatatg gcgaactggt ctgcatgacg
    25381 gctgaaatga tgctggaaca gtcgcggttg atgcacctgt tggcgcagga tagccgtttg
    25441 cgggaagaac tggtgatgaa cctgattcag gctgaggaga atactcccgc acttactgaa
    25501 tgggcgcaac ggctggggat cgatctcaat caaccgcgag tagtggctat tgttgaggtc
    25561 gacagcggtc agcttggcgt ggacagcgca atggcggagt tacaacaact gcaaaatgcg
    25621 ctgactacgc ccgagcgtaa taatctggtg gcgattgtct cgctgaccga aatggtggtg
    25681 ttgaaaccgg cgttgaactc ttttgggcgc tgggatgcag aagatcatcg taagcgagtt
    25741 gaacaactga ttacccgcat gaaagagtat ggccagctgc gttttcgcgt ttcgctgggc
    25801 aactatttta ccggtcctgg cagtattgcc cgatcctatc gtacggcgaa aacgacgatg
    25861 gtggtgggta aacagcggat gccagaaagt cgctgctatt tttatcagga tctgatgtta
    25921 cctgtgttac tcgacagttt gcgtggcgac tggcaggcca atgaactggc gcgaccgctg
    25981 gcgcggctga aagcgatgga caataatggc ttgctgcgac gaacgctggc ggcgtggttt
    26041 cgccacaatg tgcaaccgct ggctacgtca aaggcgctgt ttattcatcg taatactctg
    26101 gagtatcggc ttaatcgtat atcggaactg accgggcttg atttgggtaa ttttgatgac
    26161 aggttgctgc tgtatgtggc gttgcagctg gatgaagagc ggtaggttat gcgttaaggt
    26221 cgaggcggcg ctggctcatt cccctcagcc cgaagggtag aggggaatgt ccgagttaac
    26281 cgtcagttcg gcattattta cgcgttagtt tttccagatc ggcttcaatc tcgctgatct
    26341 tattggttac gactgactcc aggtgacgta agtcgtcgag gatcttacgc ttaagatcaa
    26401 cttcgctgcg gtcacgctgg cagatttgat caagctcatc aatgatatac cgtagattcg
    26461 ggctgatttc ctggacttct ttataaccct gacccacacc atcagcgacg accgttttac
    26521 gctgacgcgg atatttaaac ttaacgctct tggcgaaaaa ctcgcctttg tctttctgga
    26581 aatagatttt cagaatatcg ttgttggctt cctgccggag gctgtaacga tcaatttctt
    26641 caggattggt aatacccaga cttttcagat tgtcgtacat agcgttacct caaaatgagt
    26701 cagtaaattg tgcttatttt agcatttggc ctggcccgcc cggctcgact gtgatcgcag
    26761 aaagcctttt tcttccagac ttctatcatg gcgtaaaaat caaaaaatta cctgctttat
    26821 tgtggcgata ttccatggga tggattaaca catttatctt cgttttgcga attacatgac
    26881 agtccgaaac aagatgaaaa agtatttaaa aaagggggga agaagaatga gtaggacgaa
    26941 ttattctgag ccagaaagca tacgggcagc atcatgctgc ccgtatatgt actcttaatc
    27001 gatggtacgc agcagttcgt taatgccgac tttgccgcga gttttcgcgt caactttctt
    27061 aacgataacc gcacagtaga ggctgtattt gccatctttc gacggcagat tacctgaaac
    27121 aaccacagac cccgccggaa cgcgaccgta gtggatttcg ccggtttcac ggtcgtaaat
    27181 acgggtgctc tgaccaatgt atacgcccat agagatgacg gaaccttctt cgacaatcac
    27241 cccttcaacc acttcggagc gtgcgccaat gaagcagtta tcttcaataa tggtcgggtt
    27301 agcctgcagc ggctccagca cgccgccgat gcccacgcca ccggaaaggt ggacgttttt
    27361 accaatctgc gcacaagaac cgacggtcgc ccaggtatca accatcgtgc cttcatcaac
    27421 atatgcgccg atgttgacgt aagacggcat cagcacggtg ttacgggcaa taaatgcacc
    27481 ctgacgtacc gccgctggtg gcacaacgcg gaagccttct ttctgaaaac gcgcttcgtc
    27541 gtagtcggcg aatttcatcg gcactttatc gaagtagcgg ctttctgccc cttcgatcac
    27601 ctgattatca ttaatacgga aagagagcag caccgctttt ttcaaccact gatgcgtcac
    27661 ccactgaccg tcaatttttt ccgctacacg cagtgcgccg gaatccagca gggcgatcac
    27721 ctgatttacc gcttcgcggg ttacggtgtc tgcattggct ggcgtgatct cggcacggcg
    27781 ttcaaaagcg gtttcaataa tgttctgtaa ctgctgcatt gttaaactct tttcatatca
    27841 gtaaacacat caccctttat cgtttggatt gagggcctct gtcaaccgct gatgcacttc
    27901 ctgctgcaac tcgttattaa gcgcacgccg gtcagcggtg gcaattatga ataaatcttc
    27961 tactcgctcg ccaatggttg taattcgggc gccatgaagc gaaattccca gatcggcaaa
    28021 aattttcccg actctcgcca gcagtcctgg ttggtcgagc gcgataagct caaggaacga
    28081 tttgcggtcg gtatgggttg gcaaaaacgt cacttcggtt tcaacagtaa agtgacgtag
    28141 tttagccggt tgacgacgcg gctgtggtgg ctgccagcta ctttgtgtca gtacctgctc
    28201 cagaccaaac cgaataacct catgacgatc tgcggatagc gggctaccat cgggttccag
    28261 cacgatgaag gtatccatcg ccataccgtc gcgagtggtg aaaatttgtg cgtcgtgaac
    28321 acttaaattg cggcggtcta attcggcaca gacggcggca aacagatagg ggcggtccgg
    28381 gctccagata aaaatctcgg tgcctccacg cgtagcctgc gggctaagca ataccagcgg
    28441 tttgcttaaa tcatgctgta ataaatggcg ggcatgccag gccagttgat ttgggctatg
    28501 gcggacaaaa tagttagcac gacagcgtga ccaaatttgg tgcagcgcct cttcgtcgat
    28561 gttatccatg cgcagtagtg ccagtgcctg gagttggtga tggcgaaccc gttcgcgcat
    28621 atccggcgtg ttttgcattc cgcgtcgtag ctgtttttcg gtggcaaaat agagttcgcg
    28681 caacagactt tgcttccagc tattccacag cgtttcgttg gtggcgcaaa tgtcagccac
    28741 tgtcaggcat accagatagc gcagacgatt ttccgtttga acttcttcgg caaactgctt
    28801 gatgacttca gggtcctgaa tatcgcggcg ttgtgctgtc accgacatca gcaagtgctg
    28861 gcgaaccagc caggcaacca gctgtgtttc gcgtgagttc agcccgtgga gttctgcaaa
    28921 atgcactaca tcctgagcac cgagaatgga gtggtcgccg ccgcgtcctt tggcgatatc
    28981 gtgaaacagc gcggcgatga aaatcagctc agttgacggc aggcgcggcc agacgtccac
    29041 acacaacgga tggcgctggc gcgtttcttc actggcaaaa ctctccagtt tcagcatcac
    29101 gcggatagta tgttcatcca ccgtgtaggc gtggaacaga tcaaactgca tctgcccgac
    29161 gatatgcgac cattgcggca tatacgcgcc gagcacgcta tggcgatgca ttggcaatag
    29221 cccgcgccgc accgctccgg ggtgacgcag aatgctcaaa aacagttttc gtgcttccgg
    29281 aatattacac agcggttgtt gcagatggcg acgggcatgg cgtaactggc gcagcgtggt
    29341 ggagtaaatg ccggtgatcg cactgttgcg caccatggtg tagaacatac gcaagatggc
    29401 ttccggctgg cgcataaata gtgtttcatc acgcaggtcg attagcgtac cgcgtagctg
    29461 aaactcatcg tcgattggac gtggtttttc gtcggcggga agggcgagga tggcttcatc
    29521 gaacagttgc agcagcatct ggttgagttc actgacgcgg cgtgtaacgc ggaagtaatc
    29581 cttcatcatc cgctcgaccg gttcgttgcc ttcaccactg taattcagac gctgagcgac
    29641 gctaagttgg cgatcgaata acaggcgatt atcgtaacgg ctgacgacca gatgcagggc
    29701 aaagcgaata cgccacagta tatgcagaca ttcgtttaat tccgcccgct ccgctgaggt
    29761 taagaagcca aacccaacca tttcatccag cgatgttgcg ccaaaatgac ggcgggccac
    29821 ccattgcaga gtgtggatat cgcgcaagcc gccagggcta cttttgatgt ctggttcaag
    29881 attgtagctg gtgccgtggt aacgctgatg gcgctggttc tgttcttcaa ctttcgccgc
    29941 gtagaacttg tcggaaggcc agaatccttc gctaaaaata tgtttttgca gttcgaggaa
    30001 gagcgcaaca tcgccaatta ataagcggga ttcgattaaa ttggtggcga cggttaaatc
    30061 cgataaccct tccagcatgc actcttcaag cgtgcgcaca ctatgaccga cttccagctt
    30121 tacatcccag agcagcgtta acagctcgcc cactttttgc gcctgatcgt ccgggagctt
    30181 tttacggctt aaaatcagta aatcgatgtc agaaagtgga tgcagctcgc cacgaccgta
    30241 gccaccgacg gcgaccaatg ccaggtcggc aatctggctg aatcccgctt caatccataa
    30301 tcgttgcagg agctggtcga taaactcggt gcgcgcctca atcaactgtt ctgcagagat
    30361 cccattgtca aaggcatcac ccagccaacg ctggaaagta tcgatatggg cttttatccc
    30421 accgacggtt aattcatcac ggggccagac gcatggattt tgcggttgac cgggcagggt
    30481 ggggagagcg gtgtttgcgt actgttctgg aagggtattc attgtgcgcc acccataaga
    30541 ttaaattatc gcattaaaaa agccggcatt cgccggcttc atcttattcg tcgtgcgaga
    30601 ttatcgccgg gatggtgtca tccttgcgta gcgtcagaat ttcgcagccg ttatcagtca
    30661 ccacaatagt atgctcatat tgtgcagaca agctgcgatc tttggttttt accgtccagc
    30721 catctttcat ggtgcggatc tcttttttac ccgcgttgac cattggctcg atggtgaacg
    30781 tcatcccagg tttcagtacg acgttggttt cacgggagtc atagtgcagc acctgcggtt
    30841 cttcatggaa gccgcgacca ataccgtgtc cgcaatattc acgaacgacg gagaagcctt
    30901 ctgcttcgac aaatttctga atcgccgcac cgatttcgcg cagattaatg cctggtttga
    30961 ccatgcgcag cgccaggtac aggctttctt gcgtgatgcg gcacagacgt tcgcccatga
    31021 tggtcggctt accgacgata aacattttcg aggtatcgcc gtggaaacca tctttgatta
    31081 cggtgacatc aatgttaacg atatcgccat ctttcagcag cttagcatcg tccgggatac
    31141 cgtggcacac cacttcatta atagagatgc aaacggattt cggatagccg tgatagccga
    31201 ggcaggcaga aaccgcgtgt tgttcattaa caatgtaatc attacagatg cgatccagct
    31261 cgccggtgct gacgcccggt ttaacatacg gttcgatcat ctccagcact tcggcagcca
    31321 gtcggccagc gacgcgcatt ttttcgatat cttctggggt cttgattgag atagccatta
    31381 attctgtcca tcagcgtcgg tgataccgac aatatatatg taagtgccgt caatggtatc
    31441 acacccgggc aaattgagaa tcattctgaa tttcgccaaa cgtgccactg aaggttttct
    31501 ataatagaaa attcgacatc tgatgctgta cacagcgcca acaattattg gtgtccatga
    31561 cgtatttgtg gtataaagcg cgccggactt ccgatccatt tcgtatacac agactggacg
    31621 gaagcgacaa tctcactttg tgtaacaaca cacacgtatc ggcacatatt ccggggtgcc
    31681 ctttggggtc ggtaatatgg gatacgtgga ggcataaccc caacttttat atagaggttt
    31741 taatcatggc aactgtttcc atgcgcgaca tgctcaaggc tggtgttcac ttcggtcacc
    31801 agacccgtta ctggaacccg aaaatgaagc cgttcatctt cggtgcgcgt aacaaagttc
    31861 acatcatcaa ccttgagaaa actgtaccga tgttcaacga agctctggct gaactgaaca
    31921 agattgcttc tcgcaaaggt aaaatccttt tcgttggtac taaacgcgct gcaagcgaag
    31981 cggtgaaaga cgctgctctg agctgcgacc agttcttcgt gaaccatcgc tggctgggcg
    32041 gtatgctgac taactggaaa accgttcgtc agtccatcaa acgtctgaaa gacctggaaa
    32101 ctcagtctca ggacggtact ttcgaaaagc tgaccaagaa agaagcgctg atgcgcactc
    32161 gtgagctgga gaaactggaa aacagcctgg gcggtatcaa agacatgggc ggtctgccgg
    32221 acgctctgtt tgtaatcgat gctgaccacg aacacattgc tatcaaagaa gcaaacaacc
    32281 tgggtattcc ggtatttgct atcgttgata ccaactctga tccggacggt gttgacttcg
    32341 ttatcccggg taacgacgac gcaatccgtg ctgtgaccct gtacctgggc gctgttgctg
    32401 caaccgtacg tgaaggccgt tctcaggatc tggcttccca ggcggaagaa agcttcgtag
    32461 aagctgagta ataaggcttg ataactcccc caaaatagtt cgagttgcag aaaggcggca
    32521 agctcgagaa ttcccgggag cttacatcag taagtgaccg ggatgagcga gcgaagataa
    32581 cgcatctgcg gcgcgaaata tgaaggggga gagcccttat agaccaggta gtacacgttt
    32641 ggttaggggg cctgcatatg gccccctttt tcacttttat atctgtgcgg tttaatgccg
    32701 ggcagatcac atctccgagg attttagaat ggctgaaatt accgcatccc tggtaaaaga
    32761 gctgcgtgag cgtactggcg caggcatgat ggattgcaaa aaagcactga ctgaagctaa
    32821 cggcgacatc gagctggcaa tcgaaaacat gcgtaagtcc ggtgctatta aagcagcgaa
    32881 aaaagcaggc aacgttgctg ctgacggcgt gatcaaaacc aaaatcgacg gcaactacgg
    32941 catcattctg gaagttaact gccagactga cttcgttgca aaagacgctg gtttccaggc
    33001 gttcgcagac aaagttctgg acgcagctgt tgctggcaaa atcactgacg ttgaagttct
    33061 gaaagcacag ttcgaagaag aacgtgttgc gctggtagcg aaaattggtg aaaacatcaa
    33121 cattcgccgc gttgctgcgc tggaaggcga cgttctgggt tcttatcagc acggtgcgcg
    33181 tatcggcgtt ctggttgctg ctaaaggcgc tgacgaagag ctggttaaac acatcgctat
    33241 gcacgttgct gcaagcaagc cagaattcat caaaccagaa gacgtatccg ctgaagtggt
    33301 agaaaaagaa taccaggtac agctggatat cgcgatgcag tctggtaagc cgaaagaaat
    33361 cgcagagaaa atggttgaag gccgcatgaa gaaattcacc ggcgaagttt ctctgaccgg
    33421 tcagccgttc gttatggaac caagcaaaac tgttggtcag ctgctgaaag agcataacgc
    33481 tgaagtgact ggcttcatcc gcttcgaagt gggtgaaggc atcgagaaag ttgagactga
    33541 ctttgcagca gaagttgctg cgatgtccaa gcagtcttaa ttatcaaaaa ggagccgcct
    33601 gagggcggct tctttttgtg cccatcttgt aaattcagct aacccttgtg gggctgcgct
    33661 gaaaagcgac gtacaatgtc gctagtatta attcatttca atcgttgaca gtctcaggaa
    33721 agaaacatgg ctaccaatgc aaaacccgtc tataaacgca ttctgcttaa gttgagtggc
    33781 gaagctctgc agggcactga aggcttcggt attgatgcaa gcatactgga tcgtatggct
    33841 caggaaatca aagaactggt tgaactgggt attcaggttg gtgtggtgat tggtgggggt
    33901 aacctgttcc gtggcgctgg tctggcgaaa gcgggtatga accgcgttgt gggcgaccac
    33961 atggggatgc tggcgaccgt aatgaacggc ctggcaatgc gtgatgcact gcaccgcgcc
    34021 tatgtgaacg ctcgtctgat gtccgctatt ccattgaatg gcgtgtgcga cagctacagc
    34081 tgggcagaag ctatcagcct gttgcgcaac aaccgtgtgg tgatcctctc cgccggtaca
    34141 ggtaacccgt tctttaccac cgactcagca gcttgcctgc gtggtatcga aattgaagcc
    34201 gatgtggtgc tgaaagcaac taaagttgac ggcgtgttta ccgctgatcc ggcgaaagat
    34261 ccaaccgcaa ccatgtacga gcaactgact tacagcgaag tgctggaaaa agagctgaaa
    34321 gtcatggacc tggcggcctt cacgctggct cgtgaccata aattaccgat tcgtgttttc
    34381 aacatgaaca aaccgggtgc gctgcgccgt gtggtaatgg gtgaaaaaga agggacttta
    34441 atcacggaat aattcccgtg atggataaat aagggtaaga ttccacgtaa gtatcgcggg
    34501 ggcgttagtc tggttataag gcgttattgt tgcaggcagt ttggtcacgg ccagcgcgca
    34561 gcaaccggag cgtacaaaag tacgtgagga tggcgagcac tgcccggggc caaaatggca
    34621 aataaaatag cctaataatc cagacgatta cccgtaatat gtttaatcag ggctatactt
    34681 agcacacttc cactgtgtgt gactgtctgg tctgactgag acaagttttc aaggattcgt
    34741 aacgtgatta gcgatatcag aaaagatgct gaagtacgca tggacaaatg cgtagaagcg
    34801 ttcaaaaccc aaatcagcaa aatacgcacg ggtcgtgctt ctcccagcct gctggatggc
    34861 attgtcgtgg aatattacgg cacgccgacg ccgctgcgtc agctggcaag cgtaacggta
    34921 gaagattccc gtacactgaa aatcaacgtg tttgatcgtt caatgtctcc ggccgttgaa
    34981 aaagcgatta tggcgtccga tcttggcctg aacccgaact ctgcgggtag cgacatccgt
    35041 gttccgctgc cgccgctgac ggaagaacgt cgtaaagatc tgaccaaaat cgttcgtggt
    35101 gaagcagaac aagcgcgtgt tgcagtacgt aacgtgcgtc gtgacgcgaa cgacaaagtg
    35161 aaagcactgt tgaaagataa agagatcagc gaagacgacg atcgccgttc tcaggacgat
    35221 gtacagaaac tgactgatgc tgcaatcaag aaaattgaag cggcgctggc agacaaagaa
    35281 gcagaactga tgcagttctg atttcttgaa cgacaaaaac gccgctcagt agatccttgc
    35341 ggatcggctg gcggcgtttt gctttttatt ctgtctcaac tctggatgtt tcatgaagca
    35401 actcaccatt ctgggctcga ccggctcgat tggttgcagc acgctggacg tggtgcgcca
    35461 taatcccgaa cacttccgcg tagttgcgct ggtggcaggc aaaaatgtca ctcgcatggt
    35521 agaacagtgc ctggaattct ctccccgcta tgccgtaatg gacgatgaag cgagtgcgaa
    35581 acttcttaaa acgatgctac agcaacaggg tagccgcacc gaagtcttaa gtgggcaaca
    35641 agccgcttgc gatatggcag cgcttgagga tgttgatcag gtgatggcag ccattgttgg
    35701 cgctgctggg ctgttaccta cgcttgctgc gatccgcgcg ggtaaaacca ttttgctggc
    35761 caataaagaa tcactggtta cctgcggacg tctgtttatg gacgccgtaa agcagagcaa
    35821 agcgcaattg ttaccggtcg atagcgaaca taacgccatt tttcagagtt taccgcaacc
    35881 tatccagcat aatctgggat acgctgacct tgagcaaaat ggcgtggtgt ccattttact
    35941 taccgggtct ggtggccctt tccgtgagac gccattgcgc gatttggcaa caatgacgcc
    36001 ggatcaagcc tgccgtcatc cgaactggtc gatggggcgt aaaatttctg tcgattcggc
    36061 taccatgatg aacaaaggtc tggaatacat tgaagcgcgt tggctgttta acgccagcgc
    36121 cagccagatg gaagtgctga ttcacccgca gtcagtgatt cactcaatgg tgcgctatca
    36181 ggacggcagt gttctggcgc agctggggga accggatatg cgtacgccaa ttgcccacac
    36241 catggcatgg ccgaatcgcg tgaactctgg cgtgaagccg ctcgattttt gcaaactaag
    36301 tgcgttgaca tttgccgcac cggattatga tcgttatcca tgcctgaaac tggcgatgga
    36361 ggcgttcgaa caaggtcagg cagcgacgac agcgttgaat gccgcaaacg aaattaccgt
    36421 tgctgctttt cttgcgcaac aaatccgctt tacggatatc gctgcgttga atttatcggt
    36481 actggaaaaa atggatatgc gcgaaccaca atgtgtggac gatgtgttat ctgttgatgc
    36541 gaacgcgcgt gaagtcgcca gaaaagaggt gatgcgtctc gcaagctgag gataatccgg
    36601 ctacagagag tcgcgctatt tgttagcgta gggcttcagt gatatagtct gcgccatctg
    36661 atcgtaagta gttggcttta taaggtcagg tatgccgtgg ttttacacgg cttttttttg
    36721 tataggcttc agtattcctg agtaccgtaa accctgtcag ggaataaaaa acgcgtgatg
    36781 ttgtctgcta ctcaaccact tagcgaaaaa ttgccagcgc atggctgccg tcatgttgcg
    36841 atcattatgg acggcaatgg tcgctgggca aaaaagcaag ggaagattcg tgcctttggg
    36901 cataaagccg gggcaaaatc cgtccgccgg gctgtctctt ttgcggccaa caacggtatt
    36961 gaggcgttaa cgctgtatgc ctttagtagt gaaaactgga accgaccagc gcaggaagtc
    37021 agtgcgttaa tggaactgtt tgtgtgggcg ctcgatagcg aagtaaaaag tctgcaccga
    37081 cataacgtgc gtctgcgtat tattggcgat accagtcgct ttaactcgcg tttgcaagaa
    37141 cgtattcgta aatctgaagc gctaacagcc gggaataccg gtctgacgct gaatattgcg
    37201 gcgaactacg gtggacgttg ggatatagtc cagggagtca ggcaactggc tgaaaaggtg
    37261 cagcaaggaa acctgcaacc agatcagata gatgaagaga tgctaaacca gcatgtctgt
    37321 atgcatgaac tggcccctgt agatttagta attaggactg ggggggagca tcgcattagt
    37381 aactttttgc tttggcaaat tgcctatgcc gaactttact ttacagatgt tctctggccc
    37441 gatttcgatg aacaagactt tgaaggggcg ttaaatgcct ttgctaatcg agagcgtcgt
    37501 ttcggcggca ccgagcccgg tgatgaaaca gcctgatggg ggtcgctttt gctgaagtat
    37561 cgcctgatat ctgcttttgt gttaataccc gtcgtcatcg cggcgttgtt tctgttgccg
    37621 ccggtggggt tcgccatcgt aacgctggtg gtctgcatgc tggcagcgtg ggaatgggga
    37681 cagcttagcg gttttaccac tcgttcgcag cgagtatggt tggcggtgtt atgcgggtta
    37741 ttgttggcgc tgatgctttt tctgttgccg gaatatcacc gaaatattca tcaaccgctg
    37801 gttgaaatct cactttgggc ttcgctgggt tggtggattg tcgcgctatt gctggtgctg
    37861 ttttacccag gttccgcagc aatctggcgt aactctaaaa cattgcgcct tatttttggc
    37921 gtgctaacca ttgttccctt cttctggggc atgctggcgt tacgggcctg gcactatgac
    37981 gagaatcatt acagtggcgc aatatggctg ctctatgtca tgatcctggt atggggcgct
    38041 gactccggcg catatatgtt tggcaaattg tttggtaaac ataagctggc accgaaggtt
    38101 tctccgggta aaacctggca aggctttata ggtggactag cgaccgcagc ggtaatctca
    38161 tggggttatg gcatgtgggc gaatctcgac gtcgctcccg tcaccttact catttgctct
    38221 attgtcgcag cgttagcctc agtgctcggc gatctgaccg agagtatgtt taagcgtgaa
    38281 gcaggaatta aggacagcgg tcatttaatt ccaggacacg gtggtatttt agatcgtatt
    38341 gatagcctga cggctgcggt accggtcttt gcttgcttgt tgttactggt attcaggacg
    38401 ctttaacgga aggtaatatg ctgagttttc tctgggattt ggcttcgttc atcgttgcac
    38461 tgggtgtact tatcaccgtg catgaatttg gtcatttctg ggttgcccgg cgttgtggtg
    38521 ttcgcgttga gcgtttctca atagggtttg gtaaggcgct ctggcggcga actgataagc
    38581 tcggcaccga atatgttatc gccctgatcc cgctgggcgg ttatgtcaaa atgctggatg
    38641 agcgcgcaga accggtcgtt ccggaactcc gccaccatgc cttcaataat aaatctgtcg
    38701 gccaacgagc ggcgattatt gccgcaggtc cggttgcaaa cttcattttt gctatctttg
    38761 cctactggct ggtttttatt attggtgtgc ctggcgtacg tccggtggtt ggtgaaatag
    38821 cagccaattc gatagctgcg gaagcacaaa ttgcaccagg tacggaacta aaagccgtag
    38881 atggtatcga aacgcctgat tgggatgccg tgcgtttgca gttggtcgat aaaattggcg
    38941 atgaaagcac caccattaca gtagcgccat ttggcagcga ccaacggcgg gatgtaaagc
    39001 tcgatttacg tcactgggcg tttgagcctg ataaagaaga tccggtatct tcgctgggga
    39061 ttcgtcctcg tgggccgcaa attgaacctg tactggaaaa tgtgcaacca aactcggcgg
    39121 caagcaaggc aggtttgcaa gcaggcgaca ggatcgttaa agtcgatggt cagcccttaa
    39181 cgcagtgggt gacctttgtg atgcttgtcc gggataaccc gggtaaatcc ttagcgttag
    39241 aaatcgaaag gcagggaagt cccttgtctt tgacattaat cccggagagt aaaccgggta
    39301 atggtaaagc gattggtttt gtcggtattg agccgaaagt cattcctttg ccagatgagt
    39361 ataaagttgt acgccagtat gggccgttca acgccatcgt cgaagccacg gacaaaacgt
    39421 ggcagctgat gaagctgacg gtcagtatgc tgggaaaatt gatcaccggt gatgtgaaac
    39481 tgaacaacct cagtgggccg atctctatcg ccaagggggc tgggatgaca gcggaactcg
    39541 gggtagttta ttacctgccg tttcttgcgc ttattagcgt gaacttaggg ataattaacc
    39601 tgtttccgtt gcccgtactt gacggggggc atctgctgtt ccttgcgatc gaaaagatca
    39661 agggcggacc ggtatccgag cgggttcaag acttttgtta tcgcattggc tcgattctgc
    39721 tggtgctgtt aatggggctt gcacttttca atgatttctc tcggttatga gagttagtta
    39781 ggaagaacgc ataataacga tggcgatgaa aaagttgctc atagcgtcgc tgctgtttag
    39841 cagcgccacc gtatacggtg ctgaagggtt cgtagtgaaa gatattcatt tcgaaggcct
    39901 tcagcgtgtc gccgttggtg cggccctcct cagtatgccg gtgcgcacag gcgacacggt
    39961 taatgatgaa gatatcagta ataccattcg cgctctgttt gctaccggca actttgagga
    40021 tgttcgcgtc cttcgtgatg gtgataccct tctggttcag gtaaaagaac gtccgaccat
    40081 tgccagcatt actttctccg gtaacaaatc ggtgaaagat gacatgctga agcaaaacct
    40141 cgaggcttct ggtgtgcgtg tgggcgaatc cctcgatcgc accaccattg ccgatatcga
    40201 gaaaggtctg gaagacttct actacagcgt cggtaaatat agcgccagcg taaaagctgt
    40261 cgtgaccccg ctgccgcgca accgtgttga cctaaaactg gtgttccagg aaggtgtgtc
    40321 agctgaaatc cagcaaatta acattgttgg taaccatgct ttcaccaccg acgaactgat
    40381 ctctcatttc caactgcgtg acgaagtgcc gtggtggaac gtggtaggcg atcgtaaata
    40441 ccagaaacag aaactggcgg gcgaccttga aaccctgcgc agctactatc tggatcgcgg
    40501 ttatgcccgt ttcaacatcg actctaccca ggtcagtctg acgccagata aaaaaggtat
    40561 ttacgtcacg gtgaacatta ccgaaggcga tcagtacaag ctttctggcg ttgaagtgag
    40621 cggcaacctt gccgggcact ccgctgaaat tgagcagctg actaagatcg agccgggcga
    40681 gctgtataac ggcaccaaag tgaccaagat ggaagatgac atcaaaaagc ttctcggtcg
    40741 ctatggttat gcctatccgc gcgtacagtc gatgcctgaa attaacgatg ccgacaaaac
    40801 cgttaaatta cgcgtgaacg ttgatgcggg taaccgtttc tacgtgcgta agatccgttt
    40861 tgaaggtaac gatacctcga aagatgccgt cctgcgtcgc gaaatgcgtc agatggaagg
    40921 tgcatggctg ggaagcgatc tggtcgatca gggtaaggag cgtctgaatc gtctgggctt
    40981 ctttgaaact gtcgataccg atacccaacg tgttccgggt agcccggacc aggtagatgt
    41041 cgtctacaag gtaaaagagc gcaacaccgg tagcttcaac ttcgggattg gttacggtac
    41101 tgaaagtggc gtgagcttcc aggctggtgt gcagcaggat aactggttag gtacaggtta
    41161 tgctgttggt atcaacggga ccaaaaacga ttaccagacc tatgctgaac tgtcggtaac
    41221 caacccgtac ttcactgtag atggtgtaag cctcggtggt cgtctcttct ataatgactt
    41281 ccaggcagat gacgccgacc tgtccgacta taccaacaag agttatggta cagacgtgac
    41341 gttgggcttc ccgatcaacg aatataactc gctgcgtgca ggtctgggtt atgtacataa
    41401 ctccctgtcc aacatgcagc ctcaggttgc gatgtggcgt tatctgtact ctatgggtga
    41461 acatccgagc acctctgatc aggataatag cttcaaaacg gacgacttca cgttcaacta
    41521 tggttggacc tataacaagc ttgaccgtgg ttacttcccg acagatggtt cacgtgtcaa
    41581 cctgaccggt aaagtgacca ttcctggatc ggataacgaa tactacaaag tgacgttaga
    41641 cacggcgact tatgtgccga tcgatgacga tcacaaatgg gttgttctgg ggcgtacccg
    41701 ctggggttat ggtgatggtt taggcggcaa agagatgccg ttctacgaga acttctatgc
    41761 cggtggttcc agcaccgtgc gtggcttcca gtccaataca attggtccga aagcagttta
    41821 cttcccgcat caggccagta attatgatcc ggactatgat tacgaatgtg cgactcagga
    41881 cggcgcgaaa gacctgtgta aatcggatga tgctgtaggc ggtaacgcca tggcggttgc
    41941 cagcctcgag ttcatcaccc cgacgccgtt tattagcgat aagtatgcta actcggttcg
    42001 tacttccttc ttctgggata tgggtaccgt ttgggataca aactgggatt ccagccaata
    42061 ttctggatat ccggactata gtgatccaag caatatccgt atgtctgcgg gtatcgcatt
    42121 acaatggatg tccccattgg ggccgttggt gttctcctac gcccagccgt tcaaaaagta
    42181 cgatggagac aaggcagaac agttccagtt taacatcggt aaaacctggt aagtgttctc
    42241 cacaaaggaa tgtagtggta gtgtagcgat gactttaggc gatcaatata agatcgccgg
    42301 gccacgcaaa gaactgcacc ctccggtgca aatgggatgg taaggagttt attgtgaaaa
    42361 agtggttatt agcggcaggt ctcggtttag cactggcaac ttctgctcag gcggctgaca
    42421 aaattgcaat cgtcaacatg ggcagcctgt tccagcaggt agcgcagaaa accggtgttt
    42481 ctaacacgct ggaaaatgag ttcaaaggcc gtgccagcga actgcagcgt atggaaaccg
    42541 atctgcaggc taaaatgaaa aagctgcagt ccatgaaagc gggcagcgat cgcactaagc
    42601 tggaaaaaga cgtgatggct cagcgccaga cttttgctca gaaagcgcag gcttttgagc
    42661 aggatcgcgc acgtcgttcc aacgaagaac gcggcaaact ggttactcgt atccagactg
    42721 ctgtgaaatc cgttgccaac agccaggata tcgatctggt tgttgatgca aacgccgttg
    42781 cttacaacag cagcgatgta aaagacatca ctgccgacgt actgaaacag gttaaataag
    42841 taatgccttc aattcgactg gctgatttag cgcagcagtt ggatgcagaa ctacacggtg
    42901 atggcgatat cgtcatcacc ggcgttgcgt ccatgcaatc tgcacaaaca ggtcacatta
    42961 cgttcatggt taacccaaaa taccgtgagc atttaggctt gtgccaggcg tccgcggttg
    43021 tcatgaccca ggacgatctt cctttcgcga aaagtgccgc gctggtagtg aagaatccct
    43081 acctgactta cgcgcgcatg gcgcaaattt tagataccac gccgcagccc gcgcagaaca
    43141 ttgcacccag tgcggtgatc gacgcgacgg cgaagctggg taacaacgta tcgattggcg
    43201 ctaacgcggt gattgagtcc ggcgttgaac tgggcgataa cgtgattatc ggtgccggtt
    43261 gcttcgtagg taaaaacagc aaaatcggtg caggttcgcg tctctgggcg aacgtaacca
    43321 tttaccatga gatccagatc ggtcagaatt gcctgatcca gtccggaaca gtggtaggcg
    43381 cagacggctt tggttatgcc aacgatcgtg gtaactgggt gaagatccca cagattggtc
    43441 gcgtaattat tggcgatcgc gtggagatcg gtgcctgcac aaccatcgat cgcggcgcgc
    43501 tggatgacac tattattggc aatggcgtga tcattgataa ccagtgccag attgcacata
    43561 acgtcgtgat tggcgacaat acggcggttg ccggtggcgt cattatggcg ggcagcctga
    43621 aaattggtcg ttactgcatg atcggcggag ccagcgtaat caacgggcat atggaaatat
    43681 gcgacaaagt gacggttacg ggcatgggta tggtgatgcg tcccatcact gaaccaggcg
    43741 tctattcctc aggcattccg ctgcaaccca acaaagtctg gcgcaaaacc gctgcactgg
    43801 tgatgaacat tgatgacatg agcaagcgtc tgaaatcgct tgagcgcaag gttaatcaac
    43861 aagactaacg ttccatcttt tgttcgccaa actttacggc ctgtctcatt cttacgattg
    43921 cggcaggccg tgttattatt gtcgtttctt atattttgac aggaagagta tcttgactac
    43981 taacactcat actctgcaga ttgaagagat tttagaactt ctgccgcacc gtttcccttt
    44041 cttactggtg gatcgcgtgc tggattttga agaaggtcgt tttctgcgcg cagtaaaaaa
    44101 tgtctctgtc aatgagccat tcttccaggg ccatttccct ggaaaaccga ttttcccggg
    44161 tgtgctgatt ctggaagcaa tggcacaggc aacaggtatt ctggcgttta aaagcgtagg
    44221 aaaactggaa ccgggtgagc tgtactattt cgctggtatt gacgaagcgc gcttcaagcg
    44281 cccggtcgtg cctggcgatc aaatgatcat ggaagtcact ttcgaaaaaa cgcgccgcgg
    44341 cctgacccgt tttaaagggg ttgctctggt cgacggtaaa gtagtttgcg aagcaacgat
    44401 gatgtgtgct cgcagccggg aggcctgata cgtgattgat aaatccgcct ttgtgcatcc
    44461 aaccgccatt gtggaagagg gcgcgtcgat tggcgcgaac gcacacattg gtcctttttg
    44521 tatcgttgga ccccatgtcg aaattggtga gggtaccgta ctgaaatctc acgttgtcgt
    44581 gaatggccat actaaaattg gccgcgataa tgagatttat cagttcgcct ccatcggtga
    44641 agttaaccag gatctgaaat atgctggcga accgacccgt gtggaaatcg gcgatcgtaa
    44701 ccgcattcgc gaaagcgtca ccattcatcg tggcacagtc cagggcggtg gattgacgaa
    44761 ggtgggcagc gacaacttac tgatgatcaa cgcgcacatt gcgcacgatt gtacggtagg
    44821 taaccgctgt attctcgcca acaacgcaac gctggcgggt cacgtatcgg tagacgactt
    44881 cgcgatcatc ggcggcatga ccgcagtcca tcagttctgc atcattggtg cgcatgtgat
    44941 ggttggcggt tgctccggtg tggcgcagga cgtccctcct tatgtcattg cgcagggtaa
    45001 ccacgcaacg ccgttcggtg tcaatatcga agggctgaag cgccgcggat tcagccgcga
    45061 ggcgattacc gctatccgca atgcgtataa gctgatttat cgcagcggta aaacgctcga
    45121 tgaagtgaaa ccggaaattg ctgaactggc ggaaacgtat ccggaagtga aagcttttac
    45181 cgatttcttt gcacgctcaa cgcgcggtct gattcgttaa tgactaaaca gcgtccatta
    45241 acgattgccc tggtcgccgg agaaacctcc ggcgatatcc tgggggccgg tttaatccgc
    45301 gctctgaaag aacatgtgcc caacgcccgc tttgttggcg ttgccgggcc acgaatgcag
    45361 gctgaaggct gcgaagcctg gtacgaaatg gaagaactgg cggtgatggg cattgttgaa
    45421 gtgctcggtc gtctgcgtcg cttactgcat attcgtgccg atctgacaaa gcgttttggt
    45481 gaactaaagc cagatgtttt tgttggcatt gatgcgcctg acttcaatat taccctcgaa
    45541 ggtaacctta aaaagcaggg tatcaaaacc attcattacg tcagtccatc cgtctgggcg
    45601 tggcgacaga aacgcgtttt caaaataggc agagccaccg atctggtgct cgcatttctg
    45661 cctttcgaaa aagcgtttta tgacaaatac aatgtaccgt gccgctttat cggtcatacc
    45721 atggctgatg ccatgccgtt agatccagat aaaaatgccg cccgtgatgt gctggggatc
    45781 cctcacgatg cccactgcct ggcgttgcta ccggggagcc gtggcgcaga agttgaaatg
    45841 cttagtgccg atttcctgaa aacggcccag cttttgcgcc agacatatcc ggatctcgaa
    45901 atcgtggtgc cactggtgaa tgccaaacgc cgcgagcagt ttgaacgcat caaagctgaa
    45961 gtcgcgccag acctttcagt tcatttgctg gatgggatgg gccgtgaggc gatggtcgcc
    46021 agcgatgcgg cgctactggc gtcgggtacg gcagccctgg agtgtatgct ggcgaaatgc
    46081 ccgatggtgg tgggatatcg catgaagcct tttaccttct ggttggcgaa gcggctggtg
    46141 aaaactgatt atgtctcgct gccaaatctg ctggcgggca gagagttagt caaagaatta
    46201 ttgcaggaag agtgtgagcc gcaaaaactg gctgcggcgc tgttaccgct gttggcgaac
    46261 gggaaaacca gccacgcgat gcacgatacc ttccgtgaac tgcatcagca gatccgctgc
    46321 aatgccgatg agcaggcggc acaagccgtt ctggagttag cacaatgatc gaatttgttt
    46381 atccgcacac gcagctggtt gcgggtgtgg atgaagtcgg acgcgggccg ttagttggcg
    46441 cggtcgtcac cgctgcggtg atccttgacc cggcgcgccc gattgccggg ctgaatgatt
    46501 ccaaaaagct gagcgaaaaa cgccgtctgg cgctctatga agagatcaaa gagaaagcgt
    46561 tgagctggag tctgggccgc gcggaacccc acgaaatcga cgagctgaac attcttcatg
    46621 cgaccatgct ggcgatgcag cgtgccgtcg ctgggctgca tattgcgccg gaatatgtgt
    46681 tgattgatgg taaccgctgc ccgaaattac cgatgcctgc gatggctgtg gtgaaaggcg
    46741 atagccgcgt accggaaatc agtgccgcgt ctatcctggc gaaagtgacg cgtgacgccg
    46801 aaatggcggc gctggatatt gttttcccgc aatatggttt tgcccaacac aaagggtacc
    46861 caaccgcttt tcatctggaa aaactggctg aacacggcgc gaccgaacac catcggcgca
    46921 gctttgggcc tgtcaaacgc gcactgggac ttgcgtcctg attcttgtgt cgagattaag
    46981 taaaccggaa tctgaagatg tctgaaccac gtttcgtaca cctgcgggtg cacagcgact
    47041 actcgatgat cgatggcctg gccaaaaccg caccgttggt aaaaaaggcg gcggcgttgg
    47101 gtatgccagc actggcgatc accgatttca ccaacctttg tggtctggtg aagttctacg
    47161 gagcgggaca tggcgcaggg attaagccta tcgtcggggc agattttaac gtccagtgcg
    47221 acctgctggg tgatgagtta acccacctga cggtactggc ggcgaacaat accggctatc
    47281 agaatctgac gttgctgatc tcaaaagcgt atcagcgcgg gtacggtgcc gccgggccga
    47341 tcatcgatcg cgactggctt atcgaattaa acgaagggtt gatccttctt tccggcggac
    47401 gcatgggcga cgtcggacgc agtcttttgc gtggtaacag cgcgctggta gatgagtgtg
    47461 tcgcgtttta tgaagaacac ttcccggatc gctattttct cgagctgatc cgcaccggca
    47521 ggccggatga agaaagctat ctgcacgcgg cggtggaact ggcggaagcg cgcggtttgc
    47581 ccgtcgtggc gaccaacgac gtgcgcttta tcgacagcag cgactttgac gcacacgaaa
    47641 tccgcgtcgc gatccacgac ggctttaccc tcgacgatcc taaacgcccg cgtaactatt
    47701 cgccgcagca atatatgcgt agcgaagagg agatgtgtga gctgtttgcc gacatccccg
    47761 aagcccttgc caacaccgtt gagatcgcca aacgctgtaa cgtaaccgtg cgtcttggtg
    47821 aatacttcct gccgcagttc ccgaccgggg acatgagcac cgaagattat ctggtcaagc
    47881 gtgcaaaaga gggcctggaa gagcgtctgg cctttttatt ccctgacgag gaagaacgtg
    47941 ttaagcgccg cccggaatat gacgaacgtc tggagactga acttcaggtt atcaaccaga
    48001 tgggcttccc gggctacttc ctcatcgtta tggaatttat ccagtggtcg aaagataacg
    48061 gcgtaccggt agggccaggc cgtggctccg gtgcgggttc actggtggcc tacgcgctga
    48121 aaatcaccga cctcgatccg ctggaatttg acctgctgtt cgaacgtttc cttaacccgg
    48181 aacgtgtctc catgcctgac ttcgacgttg acttctgtat ggagaaacgc gatcaggtta
    48241 tcgagcacgt agcggacatg tacggtcgtg atgcggtatc gcagatcatc accttcggta
    48301 caatggcggc gaaagcggtg atccgcgacg taggccgcgt gctggggcat ccgtacggct
    48361 ttgtcgatcg tatctcgaaa ctgatcccgc ccgatccggg gatgacgctg gcgaaagcgt
    48421 ttgaagccga gccgcagctg ccggaaatct acgaagcgga tgaagaagtt aaggcgctga
    48481 tcgacatggc gcgcaaactg gaaggggtca cccgtaacgc cggtaagcac gccggtgggg
    48541 tggttatcgc accgaccaaa attaccgatt ttgcgccgct ttattgcgat gaagagggca
    48601 aacatccggt cacccagttt gataaaagcg acgttgaata cgccggactg gtgaagttcg
    48661 acttccttgg tttgcgtacg ctcaccatca tcaactgggc gctggagatg atcaacaagc
    48721 ggcgggcgaa gaatggcgag ccgccgctgg atatcgccgc gatcccgctg gatgacaaga
    48781 aaagcttcga catgctgcaa cgctcggaaa ccactgcggt attccagctt gaatcgcgcg
    48841 gcatgaagga cctgatcaag cgtctgcaac ctgactgctt cgaagatatg atcgccctgg
    48901 tggcactgtt ccgccccggt ccgttgcaat cagggatggt ggataacttt atcgaccgta
    48961 aacatggtcg tgaagagatc tcctatccgg acgtgcagtg gcagcatgaa agcttgaaac
    49021 cggtactgga gccaacctac ggcatcatcc tgtatcagga acaggtcatg cagattgccc
    49081 aggtgctttc tggttatacc ctcggtggcg cggatatgct gcgtcgtgcg atgggtaaga
    49141 aaaagccgga agagatggct aagcagcgtt ctgtatttgc tgaaggtgca gaaaagaacg
    49201 gaatcaacgc cgaactggcg atgaaaatct tcgacctggt ggagaaattc gcgggttacg
    49261 gatttaacaa atcgcactct gcggcctatg ctttggtgtc atatcaaacg ttatggctga
    49321 aagcacacta tcctgcggag tttatggcgg cggtaatgac cgccgatatg gacaacaccg
    49381 agaaggtggt gggcctggtg gatgagtgct ggcggatggg gctgaaaatc ctgccaccag
    49441 atataaactc cggtctttac catttccacg tcaacgacga cggcgaaatc gtgtatggta
    49501 ttggcgccat caaaggggta ggtgaaggtc cgattgaggc catcatcgaa gcccgtaata
    49561 aaggcggcta cttccgcgaa ctgtttgatc tttgcgcccg taccgacacc aaaaagttaa
    49621 accggcgggt gctggaaaaa ctgatcatgt ccggggcgtt tgaccgtctt ggaccacacc
    49681 gcgcggcact gatgaactcg ctgggcgatg cgttaaaagc ggcagatcaa cacgcgaaag
    49741 cggaagctat cggtcaggcc gatatgttcg gcgtgctggc cgaagagccg gaacaaattg
    49801 aacaatccta cgccagctgc caaccgtggc cggagcaggt ggtattagat ggggaacgtg
    49861 aaacgttagg cctgtacctg accggacacc ctatcaacca gtatttaaaa gagattgagc
    49921 gttatgtcgg aggcgtaagg ctgaaagaca tgcacccgac agaacgtggt aaagtcatca
    49981 cggctgcggg gctcgttgtt gccgcgcggg ttatggtcac caagcgcggc aatcgtatcg
    50041 gtatctgcac gctggatgac cgttccgggc ggctggaagt gatgttgttt actgacgccc
    50101 tggataaata ccagcaattg ctggaaaaag accgcatact tatcgtcagc ggacaggtca
    50161 gctttgatga cttcagcggt gggcttaaaa tgaccgctcg cgaagtgatg gatattgacg
    50221 aagcccggga aaaatatgct cgcgggcttg ctatctcgct gacggacagg caaattgatg
    50281 accagctttt aaaccgactc cgtcagtctc tggaacccca ccgctctggg acaattccag
    50341 tacatctcta ctatcagagg gcggatgcac gcgcgcggtt gcgttttggc gcgacgtggc
    50401 gtgtctctcc gagcgatcgt ttattaaacg atctccgtgg cctcattggt tcggagcagg
    50461 tggaactgga gtttgactaa tacaggaata ctatgagtct gaatttcctt gattttgaac
    50521 agccgattgc agagctggaa gcgaaaatcg attctctgac tgcggttagc cgtcaggatg
    50581 agaaactgga tattaacatc gatgaagaag tgcatcgtct gcgtgaaaaa agcgtagaac
    50641 tgacgcgtaa aatcttcgcc gatctcggtg catggcagat tgcgcaactg gcacgccatc
    50701 cacagcgtcc ttataccctg gattacgttc gcctggcatt tgatgaattt gacgaactgg
    50761 ctggtgaccg tgcgtatgca gacgataaag ctatcgtcgg tggtatcgcc cgtctcgatg
    50821 gtcgtccggt gatgatcatt ggtcatcaaa aaggtcgtga aaccaaagaa aaaattcgcc
    50881 gtaactttgg tatgccagcg ccagaaggtt accgcaaagc actgcgtctg atgcaaatgg
    50941 ctgaacgctt taagatgcct atcatcacct ttatcgacac cccgggggct tatcctggcg
    51001 tgggcgcaga agagcgtggt cagtctgaag ccattgcacg caacctgcgt gaaatgtctc
    51061 gcctcggcgt accggtagtt tgtacggtta tcggtgaagg tggttctggc ggtgcgctgg
    51121 cgattggcgt gggcgataaa gtgaatatgc tgcaatacag cacctattcc gttatctcgc
    51181 cggaaggttg tgcgtccatt ctgtggaaga gcgccgacaa agctccgctg gcggctgaag
    51241 cgatgggtat catcgctccg cgtctgaaag agctgaaact gatcgactcc atcatcccgg
    51301 aaccgctggg cggcgctcac cgtaacccgg aagcgatggc ggcatcgttg aaagcgcaac
    51361 tgctggcgga tctggccgat ctcgatgtgt taagcactga agatttaaaa aatcgtcgtt
    51421 atcagcgcct gatgagctac ggttacgcgt aattcgcaaa agttctgaaa aagggtcact
    51481 tcggtggccc ttttttatcg ccacggtttg agcaggctat gattaaggaa ggattttcca
    51541 ggaggaacac atgaacatca ttgccattat gggaccgcat ggcgtctttt ataaagatga
    51601 gcccatcaaa gaactggagt cggcgctggt ggcgcaaggc tttcagatta tctggccaca
    51661 aaacagcgtt gatttgctga aatttatcga acataaccct cgaatttgcg gcgtgatttt
    51721 tgactgggat gagtacagtc tcgatttatg cagcgatatc aatcaactta atgaatatct
    51781 cccgctttat gccttcatca acacccactc gacgatggat gtcagcgtgc aggatatgcg
    51841 gatggcgctc tggttttttg aatatgcgct ggggcaggcg gaagatatcg ccattcgtat
    51901 gcgtcagtac accgacgaat atcttgataa cattacaccg ccgttcacga aagccttgtt
    51961 tacctacgtt aaagagcgga agtacacctt ttgtacgccg gggcatatgg gcggcaccgc
    52021 atatcaaaaa agcccggttg gctgcctgtt ttatgatttt ttcggcggga ataccctcaa
    52081 ggctgatgtc tctatttcgg tcaccgagct tggttcgttg ctcgaccaca ccgggccaca
    52141 cctggaagcg gaagagtaca tcgcgcggac ttttggcgcg gaacagagtt atatcgttac
    52201 caacggaaca tcgacgtcga acaaaattgt gggtatgtac gccgcgccat ccggcagtac
    52261 gctgttgatc gaccgcaatt gtcataaatc gctggcgcat ctgttgatga tgaacgatgt
    52321 agtgccagtc tggctgaaac cgacgcgtaa tgcgttgggg attcttggtg ggatcccgcg
    52381 ccgtgaattt actcgcgaca gcatcgaaga gaaagtcgct gccaccacgc aggcacaatg
    52441 gccggttcat gcggtgatta ccaactccac ctatgatggc ttgctctaca acaccgactg
    52501 gatcaaacag acgctggatg tcccgtcgat ccacttcgat tccgcctggg tgccgtacac
    52561 ccattttcat ccgatctacc aggggaaaag tggtatgagc ggcgagcgtg ttgcgggaaa
    52621 agtgatcttc gaaacgcaat cgacccacaa aatgctggcg gcgttatcgc aggcttcgct
    52681 gatccacatt aaaggcgagt atgacgaaga ggcgtttaac gaagccttta tgatgcatac
    52741 caccacctcg cccagttatc ccattgttgc ttcggttgag acggcggcgg cgatgctgcg
    52801 tggtaatccg ggcaaacggc tgattaaccg ttcggtggag cgggcgctgc attttcgcaa
    52861 agaggtccag cggttgcggg aagagtctga cggttggttt ttcgatatct ggcaaccgcc
    52921 gcaggtggat gaagccgaat gctggcccgt tgcgccaggc gaacagtggc acggctttaa
    52981 cgatgcggat gccgatcata tgtttctcga tccggttaaa gtcactattt tgacaccggg
    53041 gatggacgaa cagggcaata tgagcgagga ggggatcccg gcggcgctgg tggcaaaatt
    53101 cctcgacgaa cgtgggatcg tagtagagaa aaccggccct tataacctgc tgtttctctt
    53161 tagtattggt atcgataaaa ccaaagcaat gggattattg cgtgggttga cggaatttaa
    53221 acgctcttac gatctcaacc tgcggatcaa aaatatgcta cccgatctct atgctgaaga
    53281 tcccgatttc taccgcaata tgcgtattca ggatctggcg caagggatcc ataagctgat
    53341 tcgtaaacac gatcttcccg gtttgatgtt gcgggcattc gatactttgc cggaaatgat
    53401 catgacgcca catcaggcat ggcagagaca gattaaaggt gaagtagaaa ccattgcgct
    53461 ggaacaactg gtcggtagag tatcggcaaa tatgatcctg ccttatccac cgggcgtacc
    53521 gctgttgatg ccaggagaaa tgctgaccaa agagagccgc acggtactcg attttctact
    53581 gatgctttgt tccgtagggc aacattaccc cggttttgaa acggatattc acggcgcgaa
    53641 acaggacgaa gacggcgttt accgcgtacg agtcctaaaa atggcgggat aacttgccag
    53701 agcggcttcc gggcgagtaa cgtgctgtta acaaataaag gagacgttat gctgggttta
    53761 aaacaggttc accatattgc gattattgcg acggattatg cggtgagcaa agctttctac
    53821 tgcgatattc ttggtttcac gctgcaaagc gaagtctatc gcgaagcgcg cgactcgtgg
    53881 aaaggggatt tggcgcttaa tgggcaatat gtgattgagc ttttctcttt tccgttcccg
    53941 ccggagcgcc ccagccgacc ggaagcttgc ggcctgcgtc atctggcttt tagcgttgat
    54001 gacatcgatg cggcagtggc gcaccttgaa agccataacg tgaagtgtga agccatccgt
    54061 gtcgatccat acacgcaaaa acgcttcacc ttctttaacg atccggacgg gctgccgttg
    54121 gaactgtatg agcagtaagg cttgtcatcg ccgcatttgc ccggtaacgt gccgggcatt
    54181 gctactgtaa aatcgcacca tcatgacact cacgctcaat agacaacttc tcacctcacg
    54241 ccagattctg gtggccttta gcggcgggct tgactccacc gttctgctgc atcagttggt
    54301 gcagtggcgg acggaaaatc cgggcgtcac tctgcgcgct attcatgtgc atcacggttt
    54361 aagtgccaat gccgatgcct gggttacgca ttgcgaaaac gtctgccaac agtggcaggt
    54421 gccgctggtg gtcgaacgcg tacaacttgc gcaagagggg ctggggattg aggcccaagc
    54481 gcggcaggcg cgttatcagg catttgcccg taccctattg cccggcgaag tgctggtcac
    54541 cgcgcaacat ctcgacgatc aatgtgaaac ctttctgctg gcgctaaaac gcggtagcgg
    54601 ccctgccggg ctttcggcta tgggggaagt ctcggagttt gccggaacac ggcttatccg
    54661 cccgttgctt gcccgcacgc ggggggaact ggcgcagtgg gcattggcgc atggtttacg
    54721 ctggattgaa gacgaaagta atcaggacga ctcatacgat cgtaactttc tgcgcctgcg
    54781 cgtagtgccg ttattgcagc agcgttggcc gcattttgcc gaagcaacgg cccgcagcgc
    54841 cgcactttgt gctgaacaag agagtctgtt ggatgaactg ctggcggatg atttagcaca
    54901 ctgccaaacg tcgcagggga cgctgcagat tgcgccaatg ctagcgatga gtgatgcccg
    54961 tcgcgcggcg attatccgcc gctggctggc agggcagaat gcaccgatgc cttcccgcga
    55021 cgcgctggtg cggatctggc aggaagtggc gctggcgcgg gaagatgcct ccccctgttt
    55081 acgttttggc gcgtttgaaa tacggcgcta tcagtcgcaa ctgtggtgga ttaactccgt
    55141 cactgggcaa agcgaaacca ttgtgccgtg gcagacgtgg ctacaaccgc tggaattacc
    55201 ggcggggctg ggaagtgtac agcttactgc gggaggcgat attcgccctc cgcgtgcaga
    55261 cgaagcggtc agcgtgcgtt tcaaagcgcc aggattgctg catattgtcg ggcgtaacgg
    55321 cggacgtaag ctaaagaaaa tctggcaaga gctgggcgtg ccgccgtggc tacgtgacac
    55381 cacgccactg ctgttttatg gcgaaacgct gattgcggcg gcaggggtat ttgtgacgca
    55441 agaaggtgtg gctgaaggtg agaatggcgt aagttttgtc tggcagaaaa cgcttagtta
    55501 agtgaaagcc ggataagacg catcaaacgt cgcatccggc gaaagtcaat caggactcgc
    55561 tcaccaccac cgtaccgatt tccgggtggc taaagctggt aattttatcc agacgtagct
    55621 cacgggtttc gccagcggcc tcgacgacca gatactccac atttttgcgg gagactaaat
    55681 cgctggcttt tgcctgcaat ttttcgccat ctttcagctc aagtgtcagc attaaatgat
    55741 gctggcaggc gagctcaaga ttatcgtaat catcacaatt gattggttga tacgtatcat
    55801 tcattgacat aatcgctcac cagtaagttt gccgcagcgt atgctgcttt ttccctgaca
    55861 gcctcagaaa gggcgtcgtc ggcagccatt tcattcagca ctttcaaaac gcagcccagc
    55921 gcgtccggaa cgtatcctaa gtctccgctg gcgatttccg cgtaccgctt gcgtattaac
    55981 tcacaatatt tttccacatg ccctcctgtc agcactctga cttaaccgtg gatgcaagtc
    56041 taagcctacg aagataaact ctgtttcgca aggtgactat accacactca tttctgcaat
    56101 atcagcgccg caactgcacg tattccgtta caatggcctc ctgattcgaa aggagttttc
    56161 ttatggcgct taaagcgaca atttataaag cgacggttaa tgtggccgat ctcgaccgca
    56221 accagtttct cgatgcctct ctgacgctgg cgcgccaccc ttcagaaacc caggagcgta
    56281 tgatgctgcg cttgctggcg tggctgaaat atgctgatga acgtctgcaa tttacccgtg
    56341 gtttgtgtgc cgatgatgag ccggaagcgt ggctgcgtaa cgatcacctg ggcattgatt
    56401 tgtggattga gctggggctg ccggatgagc ggcggattaa gaaagcctgc acccaggccg
    56461 cagaagtggt gctgtttacc tataatagtc gggcggcgca aatctggtgg cagcaaaatc
    56521 agagcaaatg tgtgcagttt gccaatcttt ccgtctggta tctggacgat gaacaactgg
    56581 cgaaagtaag cgcctttgcc gatcgtacca tgacgctgca ggcaacgatt caggatggcg
    56641 tgatctggtt atcggacgat aagaataatc tggaagttaa cttaaccgcc tggcaacaac
    56701 cttcatgatt gtgatttccc gacatgttgc tattcccgat ggtgagcttg agatcaccgc
    56761 cattcgtgcg cagggcgcgg gcgggcagca tgttaataag acctcaacgg ccattcatct
    56821 gcgttttgac attcgggcgt ccagcctgcc agagtattac aaagagcgtc tactcgccgc
    56881 cagccatcat ttgatcagca gtgatggcgt gattgtcatt aaggcacagg aataccgcag
    56941 tcaggaactg aaccgcgaag ccgcgctagc ccggctggtg gcagtgatta aagatttaac
    57001 aacagaacaa aaagcccgac gacccacgcg gcccacccgt gcatcgaaag agcgcaggct
    57061 ggcatcgaaa gcacaaaaat caagcgtgaa ggcgatgcgc ggcaaagtac gcagcggtcg
    57121 ggaataaaaa gaaggaatag atggtgaaaa aagcgatagt gacagcgatg gctgtaatca
    57181 gcctctttac tctgatggga tgtaataatc gggccgaagt tgatgcgctt tctccggcgc
    57241 aggctgccga actgaaaccg atgccgcaaa gttggcgcgg cgtgctgccg tgtgctgatt
    57301 gcgaaggaat cgaaacctct ctgttcctcg aaaaagacgg aacatgggtg atgaatgagc
    57361 gttatctcgg tgctcgtgaa gaaccttcct ccttcgcttc ctacggtaca tgggcgcgaa
    57421 ccgctgacaa gctggtatta accgacagca aaggtgaaaa gtcatattat cgggcgaaag
    57481 gcgatgcgct ggagatgctc gatcgtgaag gcaatccgat tgaatcgcag ttcaactata
    57541 cgctggaacc ggcacaatcc agtttaccta tgacgccgat gaccctgcga ggcatgtatt
    57601 tttatatggc tgatgcggcg accttcactg attgcgcgac cggaaaacgt ttcatggtag
    57661 cgaataacgc agagctggag cgtagctacc tggctgcgcg cggtcacagt gaaaaaccgg
    57721 tgttactgtc agtagaaggt cactttacgc ttgaggctaa tccggatact ggtgcgccaa
    57781 ccaaagtatt agcgcccgat acggcgggta aattttaccc gaataaggat tgcagtagtt
    57841 tggggcagta aacctgctta aggcagaaac aaacgcaaaa ttgcctgatg cgct
//
LOCUS       DAPHDW010000012        57126 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.012, whole genome shotgun sequence.
ACCESSION   DAPHDW010000012 DAPHDW010000000
VERSION     DAPHDW010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 57126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57126
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.012"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(56..724)
                     /locus_tag="RH057_000711"
     CDS             complement(56..724)
                     /locus_tag="RH057_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HDV3127127.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYSGFLNNYSDLKET
                     TSATGKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMK
                     EAIAQRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTM
                     DTRLYFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFD
                     VNKK"
     gene            complement(1027..1620)
                     /gene="tehB"
                     /locus_tag="RH057_000712"
     CDS             complement(1027..1620)
                     /gene="tehB"
                     /locus_tag="RH057_000712"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HDV3127128.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFPFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            complement(1617..2609)
                     /gene="tehA"
                     /locus_tag="RH057_000713"
     CDS             complement(1617..2609)
                     /gene="tehA"
                     /locus_tag="RH057_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HDV3127129.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCLFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            2733..3713
                     /locus_tag="RH057_000714"
     CDS             2733..3713
                     /locus_tag="RH057_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LbetaH domain-containing protein"
                     /protein_id="HDV3127130.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKNVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGAVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVCGQCRIFGHALINQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVIRYAFIEHRAEVFDFASIEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            complement(3708..4244)
                     /gene="rimL"
                     /locus_tag="RH057_000715"
     CDS             complement(3708..4244)
                     /gene="rimL"
                     /locus_tag="RH057_000715"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HDV3127131.1"
                     /translation="MTETIKVSESLELHAVAENHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQALIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(4307..4531)
                     /locus_tag="RH057_000716"
     CDS             complement(4307..4531)
                     /locus_tag="RH057_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HDV3127132.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            complement(4671..6326)
                     /gene="opgD"
                     /locus_tag="RH057_000717"
                     /gene_synonym="mdoD"
     CDS             complement(4671..6326)
                     /gene="opgD"
                     /locus_tag="RH057_000717"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HDV3127133.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGIAP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            complement(6551..7894)
                     /locus_tag="RH057_000718"
     CDS             complement(6551..7894)
                     /locus_tag="RH057_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV3127134.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQSATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVAAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            8111..9034
                     /locus_tag="RH057_000719"
     CDS             8111..9034
                     /locus_tag="RH057_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3127135.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSK
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEDLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSASLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            complement(9072..10712)
                     /gene="trg"
                     /locus_tag="RH057_000720"
     CDS             complement(9072..10712)
                     /gene="trg"
                     /locus_tag="RH057_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HDV3127136.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEALDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVDVFRLHKHSVSAEPRGAGEPVSFATV"
     gene            11111..11260
                     /gene="hokB"
                     /locus_tag="RH057_000721"
     CDS             11111..11260
                     /gene="hokB"
                     /locus_tag="RH057_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HDV3127137.1"
                     /translation="MKHNPLVVCLLIICITILTFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
     gene            complement(11332..11505)
                     /locus_tag="RH057_000722"
     CDS             complement(11332..11505)
                     /locus_tag="RH057_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127138.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(11750..12280)
                     /gene="cybB"
                     /locus_tag="RH057_000723"
     CDS             complement(11750..12280)
                     /gene="cybB"
                     /locus_tag="RH057_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HDV3127139.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMV
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            12469..13470
                     /gene="gap"
                     /locus_tag="RH057_000724"
     CDS             12469..13470
                     /gene="gap"
                     /locus_tag="RH057_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV3127140.1"
                     /translation="MSKVGINGFGRIGRLVLRRLLEVKSNIDVVAINDLTSPKILAYL
                     LKHDSNYGPFPWSVDFTEDSLIVDGKSIAVYAEKEAKNIPWKAKGAEIIVECTGFYTS
                     AEKSQAHLDAGAKKVLISAPAGEMKTIVYNVNDDTLDGNDTIVSVASCTTNCLAPMAK
                     ALHDSFGIEVGTMTTIHAYTGTQSLVDGPRGKDLRASRAAAENIIPHTTGAAKAIGLV
                     IPELSGKLKGHAQRVPVKTGSVTELVSILGKKVTAEEVNNALKQATTNNESFGYTDEE
                     IVSSDIIGSHFGSVFDATQTEITAVGDLQLVKTVAWYDNEYGFVTQLIRTLEKFAKL"
     gene            complement(13512..14951)
                     /gene="aldA"
                     /locus_tag="RH057_000725"
     CDS             complement(13512..14951)
                     /gene="aldA"
                     /locus_tag="RH057_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HDV3127141.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLRKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNRLGEAMQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEEAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(15148..15948)
                     /locus_tag="RH057_000726"
     CDS             complement(15148..15948)
                     /locus_tag="RH057_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HDV3127142.1"
                     /translation="MNITPFPTLSPATIDAINVIGQWLAQDDFSGEVPYQADCVILAG
                     NAVMPTIDAACKIARDQQIPLLISGGIGHSTTFLYSAIAQHPHYNTIRTTGRAEATIL
                     ADIAHQFWHIPHEKIWIEDQSTNCGENARFSIALLKQAVERVHTAIVVQDPTMQRRTM
                     ATFRRMTGDNPDAPRWLSYPGFVPQLGNNADSVIFINQLQGLWPVERYLSLLTGELPR
                     LRDDSDGYGPRGRDFIVHVDFPAEVIHAWQTLKHDAVLIEAMESRSLR"
     gene            complement(16220..20122)
                     /gene="hrpA"
                     /locus_tag="RH057_000727"
     CDS             complement(16220..20122)
                     /gene="hrpA"
                     /locus_tag="RH057_000727"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HDV3127143.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHGVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTALGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DPALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLSYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGVTVDREDWHWDQVPDHLKIT
                     FRVVDDKNKKLKEGRSLQDLKDALKGKVQETLSAVADDGIEQSGLHIWSFGQLPESYE
                     QKRGNYKVKAWPALVDERDSVAIKLFDNPLEQKQAMWNGLRRLLLLNIPSPIKYLHEK
                     LPNKAKLGLYFNPYGKVLELIDDCISCGVDQLIDANGGPVWTEEGFAALHEKVRAELN
                     DTVVDIAKQVEQILTAVFNINKRLKGRVDMTMALGLSDIKAQMGGLVYRGFVTGNGFK
                     RLGDTLRYLQAIEKRLEKLAVDPHRDRAQMLKVENVQQAWQQWINKLPPARREDEDVK
                     EIRWMIEELRVSYFAQQLGTPYPISDKRILQAMEQISG"
     gene            20323..20928
                     /gene="azoR"
                     /locus_tag="RH057_000728"
     CDS             20323..20928
                     /gene="azoR"
                     /locus_tag="RH057_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HDV3127144.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWREKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYTENGPEGLITGKKAIVITSRGGIHKDGPTDLVTPYLSTF
                     LGFIGITDVKFVFAEGIAYGPEMAAKAQSDAKAAIDSIVAA"
     gene            complement(20979..22295)
                     /locus_tag="RH057_000729"
     CDS             complement(20979..22295)
                     /locus_tag="RH057_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2/dual specificity phosphatase
                     family protein"
                     /protein_id="HDV3127145.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSLFVCSTTFEQRRLVHRLILATVMACCG
                     FLLYPLKFSFIRPEVSGVTGWLFSQLELFDLPYNQSPSLHIILCWLLWRHFRQHLAER
                     WRKVCGGWFLLIAISTLTTWQHHFIDVITGLAVGMLIDWMVPVDRRWNYQKPDQRRIK
                     IALPYVVGAGSCIVLMELMVMIQLWWSVWLCWPVLSLLIIGRGYGGLGAITTGKDSQG
                     KLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKITAGVYLGAFPRHIPAQNAVLDVTF
                     EFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCALGLSRSA
                     LVVAAWLLCYGHCKTVNEAISYIRARRPQIVLTDEHKAMLKLWENR"
     gene            complement(22285..24042)
                     /locus_tag="RH057_000730"
     CDS             complement(22285..24042)
                     /locus_tag="RH057_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HDV3127146.1"
                     /translation="MENSRIHGEHFFTSSDNTALFYRHWPALQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTVFYAWDARGHGQTSGPRGYSPSLARSVQDVDEFVRFAASDS
                     QVGLDEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIIRDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFISRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQA
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLEMIAGVLTSHKDGKPWVMRVRSQGEMDSLVRDAGFDKCT
                     QRIDEWGIFTVSMAVRRDN"
     gene            complement(24058..24954)
                     /locus_tag="RH057_000731"
     CDS             complement(24058..24954)
                     /locus_tag="RH057_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HDV3127147.1"
                     /translation="MLEKSLATLFALLILATLINHFLIWRLPERKEDEVTLRIRTWWG
                     IVICFSLVISGPRWMTLTFFALISFLALKEYCTLISVHFPRWLYWVIPLNYLFIGFNC
                     FELYLLFIPLAGFLILATGQVFMGDPSGFLHTVSAIFWGWIMTVFALSHAAWLLMLPT
                     TNIQGGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLVGGVITTMI
                     ASLIIGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLPGHGGLL
                     DRIDSLIFTAPVFFYFIRYCCY"
     gene            complement(24954..25559)
                     /locus_tag="RH057_000732"
     CDS             complement(24954..25559)
                     /locus_tag="RH057_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV3127148.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLAALALSLLTGL
                     LLMLVAQPILFMLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLLWTAINRCRSVLLMRAER"
     gene            complement(25730..28036)
                     /locus_tag="RH057_000733"
     CDS             complement(25730..28036)
                     /locus_tag="RH057_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HDV3127149.1"
                     /translation="MFKPRICSWIGLLPLFMLSLPVQAELRCVANAVNIEPFFSAATA
                     EDKQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLIVLGNLTVKGNIS
                     TFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNPSTIKGSINARGI
                     FINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWGLINDEDALKEIF
                     KRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEGRDIATFDLAIMR
                     TLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLGYQPVVLGLLDRE
                     PLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQELYKDAWLLPES
                     IIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKLAEMKHHGQLLRM
                     TPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKSPAYFCCRDMQVI
                     HSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALTHRKNNPKADSAAYETS
                     ADMILSELISHGEVDDQMLLNATALIRLEDWDFLESALVSWDNLPVVVLKELQQNTPR
                     NDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDRIWWSTLAKSNLT
                     FFKFGALNNRHTPPAVLAAEIDPEWWIVAMNNPRFPVDVLKARLKRDPLLALELVNPE
                     LDLVRQLALNGKTRAIREQAMRKLDELY"
     gene            complement(28100..28960)
                     /gene="pdxI"
                     /locus_tag="RH057_000734"
     CDS             complement(28100..28960)
                     /gene="pdxI"
                     /locus_tag="RH057_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 4-dehydrogenase"
                     /protein_id="HDV3127150.1"
                     /translation="MSSNTFTLGTKSVNRLGYGAMQLAGPGVFGPPRDRHVAITVLRE
                     ALALGVNHIDTSDFYGPHVTNQIIREALYPYSDDLTIVTKIGARRGEDASWLPAFSPA
                     ELQKAVHDNLRNLGLDVLDVVNLRIMMGDGHGPAEGSIEASLTVLAEMQQQGLVKHIG
                     LSNVTPTQVAEARKIAEIVCVQNEYNIAHRADDAMIDALARDGIAYVPFFPLGGFTPL
                     QSSTLSDVAASLGATPMQVALAWLLQRSPNILLIPGTSSVAHLRENMAAEKLHLSEEV
                     LSTLDGISRE"
     gene            complement(29191..29781)
                     /gene="paaY"
                     /locus_tag="RH057_000735"
     CDS             complement(29191..29781)
                     /gene="paaY"
                     /locus_tag="RH057_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation protein PaaY"
                     /protein_id="HDV3127151.1"
                     /translation="MPIYQIDGLTPVVPEESFVHPTAVLIGDVILGKGVYVGPNASLR
                     GDFGRIVVKDGANIQDNCVMHGFPEQDTVVEEDGHIGHSAILHGCIIRRNALVGMNAV
                     VMDGAVIGENSIVGASAFVKAKAEMPANYLIVGSPAKAIRELSEQELAWKKQGTHEYQ
                     VLVTRCKQTLHQVEPLREVEPNRKRLVFDENLRPKQ"
     gene            complement(29763..30713)
                     /gene="paaX"
                     /locus_tag="RH057_000736"
     CDS             complement(29763..30713)
                     /gene="paaX"
                     /locus_tag="RH057_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation operon negative
                     regulatory protein PaaX"
                     /protein_id="HDV3127152.1"
                     /translation="MSKLVTFIQHAVNAVPVSGTSLISSLYGDSLSHRGGEIWLGSLA
                     ALLEGLGFGERFVRTALFRLNKEGWLDVSRIGRRSFYSLSDKGLRLTRRAESKIYRAE
                     QPAWDGKWLLLLSEGLDKSTLADVKKQLIWQGFGALAPSLMASPSQKLADVQTLLHEA
                     GVADNVICFEAQIPLALSRAALRARVEECWHLTEQNAMYETFIQSFRPLVPLLKEAAD
                     ELTPERAFHIQLLLIHFYRRVVLKDPLLPEELLPAHWAGHTARQLCINIYQRVAPAAL
                     AFVSEKGETSVGELPAPGSLYFQRFGGLNIEQEAICQFTR"
     gene            complement(30814..32127)
                     /gene="paaF"
                     /locus_tag="RH057_000737"
     CDS             complement(30814..32127)
                     /gene="paaF"
                     /locus_tag="RH057_000737"
                     /EC_number="6.2.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate--CoA ligase"
                     /protein_id="HDV3127153.1"
                     /translation="MITNTKLDPIETASVDELQALQTQRLKWTLKHAYENVPMYRRKF
                     DAAGVHPDDFRELSDLRKFPCTTKQDLRDNYPFDTFAVPMEQVVRIHASSGTTGKPTV
                     VGYTQNDIDNWANIVARSLRAAGGTPKDKIHVAYGYGLFTGGLGAHYGAERLGATVIP
                     MSGGQTEKQAQLIRDFQPDMIMVTPSYCLNLIEELERQLGGDASGCSLRVGVFGAEPW
                     TQAMRKEIERRLGITALDIYGLSEVMGPGVAMECLETTDGPTIWEDHFYPEIVNPHDG
                     TPLADGEHGELLFTTLTKEALPVIRYRTRDLTRLLPGTARTMRRMDRISGRSDDMLII
                     RGVNVFPSQLEEEIVKFEHLSPHYQLEVNRRGHLDSLSVKVELKESSLTLTHEQRCQV
                     CHQLRHRIKSMVGISTDVMIVNCGSIPRSEGKACRVFDLRNIVGA"
     gene            complement(32154..33359)
                     /gene="paaJ"
                     /locus_tag="RH057_000738"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
     CDS             complement(32154..33359)
                     /gene="paaJ"
                     /locus_tag="RH057_000738"
                     /gene_synonym="paaE"
                     /gene_synonym="ydbW"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3-oxoadipyl-CoA/3-oxo-5,6-dehydrosuberyl-CoA thiolase"
                     /protein_id="HDV3127154.1"
                     /translation="MREAFICDGIRTPIGRYGGALSGVRADDLAAIPLRELLVRNPRL
                     DAECIDDVILGCANQAGEDNRNVARMATLLAGLPQSVSGTTINRLCGSGLDALGFAAR
                     AIKAGDGDLLIAGGVESMSRAPFVMGKATSAFSRQAEMFDTTIGWRFVNPLMAQQFGT
                     DSMPETAENVAELLKISREDQDSFALRSQQRTAKAQSSGILAEEIVPVVLKNKKGVVT
                     EIQHDEHLRPETTLEQLRGLKAPFRANGVITAGNASGVNDGAAALIIASEQMAAAQGL
                     TPRARIVAMATAGVEPRLMGLGPVPATRRVLERAGLSIHDMDVIELNEAFAAQALGVL
                     RELGLPDDAPHVNPNGGAIALGHPLGMSGARLALAASHELHRRNGRYALCTMCIGVGQ
                     GIAMILERV"
     gene            complement(33359..33781)
                     /gene="paaI"
                     /locus_tag="RH057_000739"
     CDS             complement(33359..33781)
                     /gene="paaI"
                     /locus_tag="RH057_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyphenylacetyl-CoA thioesterase PaaI"
                     /protein_id="HDV3127155.1"
                     /translation="MSHKAWQNAHAMYENDACAKALGIDIISMDEGFAVVTMTVTAQM
                     LNGHQSCHGGQLFSLADTAFAYACNSQGLAAVASACTIDFLRPGFAGDTLTATAQVRH
                     QGKQTGVYDIEIVNQQQKTVALFRGKSHRIGGTITGEA"
     gene            complement(33771..35198)
                     /gene="paaC"
                     /locus_tag="RH057_000740"
     CDS             complement(33771..35198)
                     /gene="paaC"
                     /locus_tag="RH057_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase PaaC"
                     /protein_id="HDV3127156.1"
                     /translation="MMINVQTVAVIGSGTMGAGIAEVAASHGHQVLLYDISAEALTRA
                     IDGIHARLNSRVTRGKLTAETCERTLKRLIPVTDIHALAAADLVIEAASERLEVKKAL
                     FAQLAEVCPPQTLLTTNTSSISITAIAAEVKNPERVAGLHFFNPAPVMKLVEVVSGLA
                     TAAEVVEQLCELTLSWGKQPVRCHSTPGFIVNRVARPYYSEAWRALEEQVAAPEVIDA
                     ALRDGAGFPMGPLELTDLIGQDVNFAVTCSVFNAFWQERRFLPSLVQQELVIGGRLGK
                     KSGLGVYDWRAEREAVVGLEAVSDSFSPMKVEKKSDGVTEIDDVLLIETQGETAQALA
                     IRLARPVVVVDKMAGKVVTIAAAAVNPDSATRKAIYYLQQQGKTVLQIADYPGMLIWR
                     TVAMIINEALDALQKGVASEQDIDTAMRLGVNYPYGPLAWGAQLGWQRILRLLENLQH
                     HYGEERYRPCSLLRQRALLESGYES"
     gene            complement(35200..35988)
                     /gene="paaB"
                     /locus_tag="RH057_000741"
     CDS             complement(35200..35988)
                     /gene="paaB"
                     /locus_tag="RH057_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase"
                     /protein_id="HDV3127157.1"
                     /translation="MMEFILSHVEKGVMTLTLNRPERLNSFNDEMHAQLAECLKQVER
                     DDTIRCLLLTGAGRGFCAGQDLNDRNVDPTGPAPDLGMSVERFYNPLVRRLAKLPKPV
                     ICAVNGVAAGAGATLALGCDIVIAARSAKFVMAFSKLGLVPDCGGTWLLPRVAGRARA
                     MGLALLGNQLSAEQAHEWGMIWQVVDDETLADTAQQLARHLATQPTFGLGLIKQAINS
                     AETNTLDTQLDLERDYQRLAGRSADYREGVSAFLAKRSPQFTGK"
     gene            complement(35988..36755)
                     /locus_tag="RH057_000742"
     CDS             complement(35988..36755)
                     /locus_tag="RH057_000742"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dehydroadipyl-CoA hydratase"
                     /protein_id="HDV3127158.1"
                     /translation="MSELIVSRQQQVLLLTLNRPAARNALNNALLTQLVNELEAAATD
                     SSISVCVITGNARFFAAGADLNEMAEKDLAATLNDTRPQLWARLQAFNKPLIAAVNGY
                     ALGAGCELALLCDVVVAGENARFGLPEITLGIMPGAGGTQRLIRSVGKSLASKMVLSG
                     ESITAQQAQQAGLVSDVFPSDLTLEYALQLASKMARHSPLALQAAKQALRQSQEVALQ
                     AGLAQERQLFTLLAATEDRHEGISAFLQKRTPDFKGR"
     gene            complement(36752..37822)
                     /gene="paaK"
                     /locus_tag="RH057_000743"
     CDS             complement(36752..37822)
                     /gene="paaK"
                     /locus_tag="RH057_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase/reductase subunit
                     PaaK"
                     /protein_id="HDV3127159.1"
                     /translation="MTTFHSLTVAKVEPETRDAVTITFAVPQPLQEAYRFRPGQHLTL
                     KASFDGEELRRCYSICRSYLPGEISVAVKAIEGGRFSRYAREHIRQGMTLEVMVPQGH
                     FGYQPQAERQGRYLAIAAGSGITPMLAIIATTLQTEPESQFTLIYGNRTSQSMMFRQA
                     LADLKDKYPQRLQLLCIFSQETLDSDLLHGRIDGEKLQSLGASLINFRLYDEAFICGP
                     AAMMDETEAALKALGMPDKTIHLERFNTPGTRVKRSVNVQSDGQKVTVRQDGRDREIV
                     LNADDESILDAALRQGADLPYACKGGVCATCKCKVLRGKVAMETNYSLEPDELAAGYV
                     LSCQALPLTSDVVVDFDAKGMA"
     gene            complement(37830..38327)
                     /gene="paaD"
                     /locus_tag="RH057_000744"
     CDS             complement(37830..38327)
                     /gene="paaD"
                     /locus_tag="RH057_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate degradation protein PaaD"
                     /protein_id="HDV3127160.1"
                     /translation="MQRLATIAPPQVHEIWALLSQIPDPEIPVLTITDLGMVRNVTQM
                     GEGWVIGFTPTYSGCPATEHLIGAIREAMTTHGFTPVQVVLQLDPAWTTDWMTPDARE
                     RLRQYGISPPAGHSCHAHLPPEVRCPRCASVHTTLISEFGSTACKALYRCDSCREPFD
                     YFKCI"
     gene            complement(38342..39088)
                     /gene="paaC"
                     /locus_tag="RH057_000745"
     CDS             complement(38342..39088)
                     /gene="paaC"
                     /locus_tag="RH057_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetate-CoA oxygenase subunit PaaC"
                     /protein_id="HDV3127161.1"
                     /translation="MNQLTAYTLRLGDNCLVLSQRLGEWCGHAPELEIDLALANIGLD
                     LLGQARNFLSYAAELAGEGDEDTLAFTRDERQFSNLLLVEQPNGNFADTIARQYFIDA
                     WHVALFTRLMESRDPQLAAISAKAIKEARYHLRFSRGWLERLGNGTDVSGQKMQQAID
                     KLWRFTAELFDADEIDIALSEEGIAVDPRTLRAAWEAEVFAGINEATLNVPQEQAYRT
                     GGKKGLHTEHLGPMLAEMQYLQRVLPGQQW"
     gene            complement(39097..39384)
                     /gene="paaB"
                     /locus_tag="RH057_000746"
     CDS             complement(39097..39384)
                     /gene="paaB"
                     /locus_tag="RH057_000746"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit B"
                     /protein_id="HDV3127162.1"
                     /translation="MSNVYWPLYEVFVRGKQGLSHRHVGSLHAADERMALENARDAYT
                     RRSEGCSIWVVKASEIVASQPEERGEFFDPAESKVYRHPTFYTIPDGIEHM"
     gene            complement(39396..40325)
                     /gene="paaA"
                     /locus_tag="RH057_000747"
     CDS             complement(39396..40325)
                     /gene="paaA"
                     /locus_tag="RH057_000747"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit A"
                     /protein_id="HDV3127163.1"
                     /translation="MTQEERFEQRIAQETAIEPQDWMPDAYRKTLIRQIGQHAHSEIV
                     GMLPEGNWITRAPTLRRKAILLAKVQDEAGHGLYLYSAAETLGCAREDIYQKMLDGRM
                     KYSSIFNYPTLSWADIGVIGWLVDGAAIVNQVALCRTSYGPYARAMVKICKEESFHQR
                     QGFEACMALAQGSEAQKQMLQDAINRFWWPALMMFGPNDDNSPNSARSLAWKIKRFTN
                     DELRQRFVDNTVPQVEMLGMTVPDPDLHFDTESGHYRFGEIDWQEFNEVINGRGICNQ
                     ERLDAKRKAWEEGTWVREAALAHAQKQLARKVA"
     gene            40610..42655
                     /gene="paaZ"
                     /locus_tag="RH057_000748"
     CDS             40610..42655
                     /gene="paaZ"
                     /locus_tag="RH057_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetic acid degradation bifunctional
                     protein PaaZ"
                     /protein_id="HDV3127164.1"
                     /translation="MQQLASFLSGTWQSGRGRSRLIHHAISGEALWEVTSEGLDMAAA
                     RQFAIEKGAPALRAMTFIERAAMLKAVAKHLLSEKERFYALSAQTGATRADSWVDIEG
                     GIGTLFTYASLGSRELPDDTLWPEDELIPLSKEGGFAARHVLTSKSGVAVHINAFNFP
                     CWGMLEKLAPTWLGGMPAIIKPATATAQLTQAMVKSIVDSGLVPEGAISLICGSAGDL
                     LDHLDSQDVVTFTGSAATGQMLRVQPNIVAKSIPFTMEADSLNCCVLGEDVTPDQPEF
                     ALFIREVVREMTTKAGQKCTAIRRIIVPQALVNAVSDALVARLQKVVVGDPAQEGVKM
                     GALVNAEQRADVQEKVNILLAAGCEIRLGGQADLSAAGAFFPPTLLYCPQPDETPAVH
                     ATEAFGPVATLMPAQNQQHALQLACAGGGSLAGTLVTADPQIARQFIADAARTHGRIQ
                     ILNEESAKESTGHGSPLPQLVHGGPGRAGGGEELGGLRAVKHYMQRTAVQGSPTMLAA
                     ISKQWVRGAKVEEDRIHPFRKYFEELQPGDSLLTPRRTMTEADIVNFACLSGDHFYAH
                     MDKIAAAESIFGERVVHGYFVLSAAAGLFVDAGVGPVIANYGLESLRFIEPVKPGDTI
                     QVRLTCKRKTLKKQRSAEEKPTGVVEWAVEVFNQHQTPVALYSILTLVARQHGDFVD"
     gene            42903..45176
                     /gene="tynA"
                     /locus_tag="RH057_000749"
     CDS             42903..45176
                     /gene="tynA"
                     /locus_tag="RH057_000749"
                     /EC_number="1.4.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415904.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primary-amine oxidase"
                     /protein_id="HDV3127165.1"
                     /translation="MGSPSLYSARKTALALAVALSFAWQAPVFAHGGEAHMVPMDETL
                     KEFGADVQWDDYAQIFTLIKDGAYVKVKPGAQTAIVNGQPLALQVPVVMKDNKAWVSD
                     TFINDVFQSGLDQTFQVEKRPHPLNALTADEIKQAVEIVKASADFKPNTRFTEISLLP
                     PDKEAVWAFALENKPVDQPRKADVIMLDGKHIIEAVVDLQNNKLLSWQPIKDAHGMVL
                     LDDFASVQNIINNSEEFAAAVKKRGITDAKKVITTPLTVGYFDGKDGLKQDARLLKVI
                     SYLDVGDGNYWAHPIENLVAVVDLEQKKIVKIEEGPVVPVPMTARPFDGRDRVAPAVK
                     PMQIIEPEGKNYTITGDMIHWRNWDFHLSMNSRVGPMISTVTYNDNGTKRKVMYEGSL
                     GGMIVPYGDPDIGWYFKAYLDSGDYGMGTLTSPIARGKDAPSNAVLLNETIADYTGVP
                     MEIPRAIAVFERYAGPEYKHQEMGQPNVSTERRELVVRWISTVGNYDYIFDWIFHENG
                     TIGIDAGATGIEAVKGVKAKTMHDETAKDDTRYGTLIDHNIVGTTHQHIYNFRLDLDV
                     DGENNSLVAMDPVVKPNTAGGPRTSTMQVNQYNIGNEQDAAQKFDPGTIRLLSNPNKE
                     NRMGNPVSYQIIPYAGGTHPVAKGAQFAPDEWIYHRLSFMDKQLWVTRYHPGERFPEG
                     KYPNRSTHDTGLGQYSKDNESLDNTDAVVWMTTGTTHVARAEEWPIMPTEWVHTLLKP
                     WNFFDETPTLGALKKDK"
     gene            complement(45234..46733)
                     /gene="feaB"
                     /locus_tag="RH057_000750"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
     CDS             complement(45234..46733)
                     /gene="feaB"
                     /locus_tag="RH057_000750"
                     /gene_synonym="maoB"
                     /gene_synonym="padA"
                     /EC_number="1.2.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylacetaldehyde dehydrogenase"
                     /protein_id="HDV3127166.1"
                     /translation="MTEPHVAVLSQVQQFLDRQHGLYIDGRPGPAQSEKRLAIFDPAT
                     GQEIASTADANEADVDNAVMSAWRAFVSRRWAGRLPAERERILLRFADLVEQHSEELA
                     QLETLEQGKSIAISRAFEVGCTLNWMRYTAGLTTKIAGKTLDLSIPLPQGARYQAWTR
                     KEPVGVVAGIVPWNFPLMIGMWKVMPALAAGCSIVIKPSETTPLTMLRVAELASEAGI
                     PDGVFNVVTGSGAVCGAALTSHPHVAKISFTGSTATGKGIARTAADRLTRVTLELGGK
                     NPAIVLKDADPQWVIEGLMTGSFLNQGQVCAASSRIYIEAPLFDTLVSGFEQAVKSLQ
                     VGPGMSPVAQINPLVSRAHCDKVCSFLDDAQAQQAELIRGLNGPAGEGYYVAPTLVVN
                     PDAKLRLTREEVFGPVVNLVRVADGEEALQLANDTEYGLTASVWTQNLSQALEYSDRL
                     QAGTVWVNSHTLIDANLPFGGMKQSGTGRDFGPDWLDSWRETKSVCVRY"
     gene            46969..47874
                     /gene="feaR"
                     /locus_tag="RH057_000751"
     CDS             46969..47874
                     /gene="feaR"
                     /locus_tag="RH057_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FeaR"
                     /protein_id="HDV3127167.1"
                     /translation="MNPAVDNEFQQWLSQINQVCGNFTGRLLTERYTGVLETHFAKGL
                     KLSTVTTNGVNLYRTWQEVKGSDDAWFYTVFQLSGQAIMEQDERQVQIGAGDITLLDA
                     SRPCSLYWQESSKQISLLLPRTLLEQYFPHQKPVCAERLDADLPMVQLSHRLLQESMN
                     NPALSETESEAALQAMVCLLRPVLHQRESVQPRRERQFQKVVTLIDDNIREEILRPEW
                     IAGETGMSVRSLYRMFADKGLVVAQYIRNRRLDFCADAIRHAADDEKLAGIGFHWGFS
                     DQSHFSTVFKQRFGMTPGEYRRKFR"
     gene            complement(48046..48375)
                     /locus_tag="RH057_000752"
     CDS             complement(48046..48375)
                     /locus_tag="RH057_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HDV3127168.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTDINAERKASYQQLAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(48380..48565)
                     /locus_tag="RH057_000753"
     CDS             complement(48380..48565)
                     /locus_tag="RH057_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HDV3127169.1"
                     /translation="MKILLAALTSSFMLAACTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(48562..51201)
                     /locus_tag="RH057_000754"
     CDS             complement(48562..51201)
                     /locus_tag="RH057_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HDV3127170.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLAGIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWPYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLSVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLTDPTLAFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRDPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGPMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYYHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            51409..52398
                     /gene="ldhA"
                     /locus_tag="RH057_000755"
     CDS             51409..52398
                     /gene="ldhA"
                     /locus_tag="RH057_000755"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV3127171.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSRPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            52509..52931
                     /gene="hslJ"
                     /locus_tag="RH057_000756"
     CDS             52509..52931
                     /gene="hslJ"
                     /locus_tag="RH057_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HDV3127172.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     NTISEMLKEGAQVDLTANQLTLATAKQTLTYKLADLMN"
     gene            complement(52928..53194)
                     /locus_tag="RH057_000757"
     CDS             complement(52928..53194)
                     /locus_tag="RH057_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HDV3127173.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPGLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            53468..56992
                     /gene="nifJ"
                     /locus_tag="RH057_000758"
     CDS             53468..56992
                     /gene="nifJ"
                     /locus_tag="RH057_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HDV3127174.1"
                     /translation="MITIDGNGAVASVAFRTSEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISQIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQVEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYDAVYDHVEQAMNDFSAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPGG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRLEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACSGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAADLQKRYDFLAQMAGKAEKSNTD"
BASE COUNT        14418 a        15037 c        13612 g        14059 t
ORIGIN      
        1 ggggttttac ggcgcaccgg ataaaaagcg ccatctaacg atggcgcgtc tggaactact
       61 ttttgttaac gtcaaacatg gtggcatcgg tcgccatgtc atcaataact tgtttaatat
      121 tttcgaaagc cattggcgta ctttcgttat tcaggtcttt accttcgccc tgacgaacga
      181 ctttgataac cggtttatta gtcgctgcat caatcagctc accttcaaaa tagaggcgag
      241 tatccatggt acggtggcct gtagccattt gcgtccccgc aaccactaat gcaacaggaa
      301 ccacttcata gaattgcagc ccttctttgc tggtatctac accggtaatg gccccacgga
      361 aaatcagact acgcggccca gcggtggtaa ccactggttt acgctgcgct atcgcttctt
      421 tcatttcggt gttggtatag ttcaaaattt tatccagaac tttctgccct acctgggtag
      481 aaggttttgg caccggataa taagtgattg ggttccagac gatgctgtca tatttgcttt
      541 gatcaaaact cgggtctacc caacgtaaaa caggtttacc tgttgctgag gttgtttctt
      601 ttaaatcaga gtaattgttt aaaaaaccag aatatttatc tggctgggtg attttagagg
      661 cacaaccaga cagagccaac aagccgcata aagctgcaac tttcgcaaat gatgtggtac
      721 gcatgaatag tttcctgaga tgattatatt actttttaag ttatagcaaa aagcgccgct
      781 tcgtgttgtg gcaaaaaaag tgttggtgaa aataaatttt tcagcccctt ttcagacgac
      841 atagtcaatg ccgaaaaagg ggctaagtca atcatcagta gttaaatttc agtaagttaa
      901 gctgctaaaa aacttcgact actgtgatta atttcacatc ccttcgataa aaaatatgct
      961 taccgaaaag aagaacagta aatcatctca gcgtggggaa ttttcacgcg ccttattctt
     1021 agcgggtcat tttttacgtg ccagcatcgt ggcgaaacgc agtttaatac gattaccgtt
     1081 ggcgtcggtg cggtgcagct cgccgacgtc ttcattgtat ttcaccctct cccagccttc
     1141 gtaatatcga cgtaattctc cctctttgaa ggcaaacggg aagccgacgg tacatggata
     1201 atcagcggta tccatcgccg ccacaatcag gttgtaacca ccaggtttag tgcaacgttg
     1261 catattggca atcaacccgg ggatggtttt agcctcaagg aacatcagca ccacagtcga
     1321 aagaataaaa tcgtactgtc catcaaatgt gaggttattc agatcaacga cccgggtgtg
     1381 taaattatcc agattttcaa tggatttaat gcgctcgacg ttggcgatac tcatggcatt
     1441 tttatcccat gcgtcaacat cataaccatt ggctgccagg taaagactgt tacgaccatt
     1501 gccacagccc agatccagcg ttttacccgg tttaaccact ttcaccgctt ccagtacttc
     1561 agagtgtgtg cgggttaatt catatttatc agtaaaatag ttttcgtcac gaatgatcat
     1621 tctttgtcct ctgctttcat taaaacggcg cgctcggttc tgactaacaa ttttccctgc
     1681 atcagaagcg cgaaagtacg gatgagcagt attgcaataa taaaattggt aaagataaac
     1741 agcggcaccg ccagcgtatg gaaaaatcca ttatcgctgc cactccccag atgcaaaccg
     1801 gtggttgcca gtgcagatac gccgaacgag aaactccaga atgaagcatt aaatggctgg
     1861 gagagatacc atggcatcag acgtagcata aacagcagtt gcagcagtcc ataaccaaaa
     1921 agcattttcg ccagcgtatc accctcgccg ccgttgacgc tcaaccaggc actacaggcc
     1981 accagcgcag gagcgagctg aatgccgagt gatgtccgca gtgccgtggg taattctccc
     2041 gaactgcgca gacgctgcaa gatcacaggt tcaaggctta gccatgagaa aacgcctgcg
     2101 cctaaaaaca ccagaccggc gtcggtgtag cccaacgcac cacaggccat tgcgctgata
     2161 aagttgttgg caactgtcgg cagatacagt ccaggcgtgg tagcttcttc agggtgagat
     2221 ccgcgccata atcccgcagt ttgccaggcg gcataagcca actgaaccac gacaccaaaa
     2281 ctgaacaggc acaccgccag tgggcgaaac cacggaacaa aaccaatcgc caccagcatc
     2341 gtcgttgccg gaaacaaact cacaaaactg ctcagcactg gatggcgcac ttctgccagc
     2401 acgctatgcg gaaagcgtat aagtcgggta ataaatgcgc tagttaacaa tccccagatg
     2461 atcatcgcca gaatcaccag cccatctcct aaccagtggc tgacctgcca aacctggctg
     2521 gcatagcgcc aggcaaatcc catccctatc gtccccaaca caataccaaa gtagcctgcc
     2581 ggcaaattga gcactttatc gctctgcatc ttcttactca ttttgtttaa tttataaagt
     2641 atatttgaaa tgcattttat ggaattttct cgagcatagc cagagccgca gaatttgcta
     2701 cggttatagt aagtcacctg tgggaaaacg ctatgcgcaa atatcgtcta agtgaagaac
     2761 agcgagcctt tagttatcag gaagatggca ctaaaaaaaa tgtgttatta cggcagatta
     2821 tcgccattag cgattttaac gatgtgatag caggaaccgc tggcggctgg atcgatcgcg
     2881 aaacggtgtt agcacaggaa ggaaattgct ggatttacga ccagaacgct attgcgttcg
     2941 gcggagcggt gatttccggt aatacgcgca tcaccggcac cagcgtgctg tggggagagg
     3001 tttacgcaac ggataatgtc tggatcgata atagtgaaat cagccagggt gcatatataa
     3061 gtgacagcgt caccattcat gactcactgg tttgcgggca atgtagaata tttggtcacg
     3121 ccttaattaa tcaacattct atgatcgtcg ccgcacaagg cttaacgccc gaccatcaac
     3181 tcctgctcca aatttatgat cgcgcaaggg ttagcgcctc gcgtattgtc caccaggcac
     3241 aaatttatgg cgatgccgtc atccggtatg cttttattga acatcgtgcc gaagtttttg
     3301 attttgccag tattgaaggt aatgaagaga acaacgtctg gctatgcgat tgcgctaaag
     3361 tctacggtca cgcgcaggtg aaagctggca tagaagaaga tgccattcca acgattcatt
     3421 acagttcgca ggtggcggaa tatgccattg tggaaggtaa ttgtgtgtta aaacatcatg
     3481 tcttgattgg cggaaatgcc gtggtacgtg gtggaccgat tctgctcgac gagcacgttg
     3541 taatacaagg tgagagccgt attacaggtg cggtgataat tgaaaaccat gtggagttaa
     3601 ccgaccacgc cgtcgttgaa gcctttgatg gcgacacggt tcatgtccgt ggtccaaaag
     3661 tcattaacgg cgaggagcgc attacgcgaa cgccgctggc agggttatta tgaatcgata
     3721 atacgcgcgt ataagttcac atcatcatag gcatcattca ggaactcagc ctgtttcagg
     3781 caaccttcaa ggataaaacc attgcgcaaa gcgacctggt tgctttgcgg attgtccacc
     3841 cgacatttga tcacgaagcg tctaagttca ccagactggg cgtaatgatg aatcaatgcc
     3901 tgcagcgcct gagaaatgat cccctgcccc tgatgagatt cgtccagcca gtagcctatt
     3961 tcagcggttt tattcagtgg ttcaatacga ttaaacgaga taacgccgat aagttcatct
     4021 tctttgaaaa tcatgaacat tttggcatag ccgcgttgat gcaacatcac attaccctgc
     4081 accgtttttc gcgtgtcctc ttcactctga acgaactgcg gccagtttag cgactgctgt
     4141 aaccaggttt tatttttaca gattaactga taaagaggag tgacgtgatt ttctgcaaca
     4201 gcatgtaatt caagtgattc gcttactttt atcgtttcag tcatcttatc tccagtcaat
     4261 ccatgcagtt gttaaaaaag ccgcctggct ggcggcttag gaaagtttat acctctttga
     4321 cgctctcctg ctgcaggatt ttgagcatct catcttttaa ctgtagcttt tgttttttca
     4381 tgcggaccac ttccgcattg taccctcgac cgtcggaacc ttcctttctg gcaatttcat
     4441 gatcaagttt attgtgttta tcgaacaagg acataaagcg aggattttcg tttttcagac
     4501 gggatattaa atctcgatat tctggaaaca tacgcactcc ctgttagtgg ctgacaaggt
     4561 gtatctgaaa tacacaccat cagattactc atttaactta gggaaaaatg cgagcgggat
     4621 cgtgtttaac cagacggcac ctcccgaaga aggtgccgaa gaaaaaacga ttaactcatc
     4681 acgcggtcgt caacatactg acgtttatcc ggcgctggcg ggaaatactg atacagccat
     4741 gtttcactga tagcgtcgcc ctgacaacgc agatacatcc gcatatcgac cggatcagtg
     4801 gagtccgaag tcggatacca gtcaaactga atacgataac catcaatggg ttcaatatag
     4861 agaatttcga tttgcttcgc ttccccactg gaaagcgtaa tcaccggctc aatgcctttt
     4921 ggtgcggcag ctttcagatc accaccaacg aaatcgacgg caaaacgacg cgcccatttt
     4981 tcgggatagt gttcacctgg tgcccaacct tccgggaaac cgcccatgcc ggtacgcgtc
     5041 gccataacgc gcgctaatgg gcaatgaaca ggcggttgcg cactccagta cagacgatac
     5101 tggaatgcaa actcatcacc cgcctttaca gctttttctg gctgccagaa gcagacaatg
     5161 ttatccagcg tttcgcccgt tgttgggatt tccatcaggc cgatggtgcc cttaccccac
     5221 ttgttacgcg gttccaccca cagacttggg cgtttgttat accagcccat aatgtcctga
     5281 taatgggaga aatcacgatc cagttgcaat aaaccaaacc ctttcgggtt gttgtcggtg
     5341 taagcattga actgcaattt ttgcggatta ttcagcggac ggcaaatcca ctcgccgttg
     5401 ccccgccaca tggacagacg atcagagtca tgaatttgcg gatgaattgt atcgcacatc
     5461 cgacgttcat tagtaccgca gctgaacata ctggtcatcg gcgcaatgcc cagctgttta
     5521 atgtctttgc gcgcatacag gtgattttcc acatccataa tcacctgact tttctcacaa
     5581 tggatagtga acttataggc accagtaatg ctggcgctat cgagcaacgc ataaacggta
     5641 aaggtagttg cccccggttt taccgtatca aaccagaagg cggtaaagtc ggggaactct
     5701 tctttactgt cagtgtaagt gtcgatcgcc aggccgcggg ccgacaaacc gtattgatat
     5761 gtatcatcaa cggcgcggaa ataactcgcg ccgagaaatg atactacatc acggcgcgcc
     5821 agttcggggg ctttaaacac gcgaaaaccg gcaaagccga gatcgctttg cccttctaat
     5881 tgttttgtat caacacccgc atcgttgtat ttgaacaact ccgggcgaaa gtgaatttca
     5941 cgcgccagat gtgttgctgg atctacagaa aacatacgaa cgcggcgacg gaatcccatt
     6001 cccatatgga agaactgagc gtccagttga cggttctcaa cgttatgcca gagcgatttt
     6061 tcggcgtcgt attgaatact gttataagcc tgcggcgtca ttgtcgccag cgtgtcaggt
     6121 aacggacgag gcgcaccacg ccacgctgtt tgcgctaagt cgtgcgccat tgactgtaga
     6181 atggagaagt caaaacgctg ggtttgcccg tcggcaatat cagaatctgc cgcgaatgcc
     6241 gcctgagaaa aaagagaagc aatgccgctg gtaccgcaca cggcggccat agccattgaa
     6301 cctttaataa atcgtctacg atccatacct gaaagtgagt ccttctggtg ttgtgaatgg
     6361 tcccgcgctg gtcgttttca gcggcaagaa agtgcttatt tatgaccgct cactctagac
     6421 aatatttatt aataatccaa ttgttgaact gagagaatat gcataaatcg gcattttttt
     6481 tatgtcgatg ggatgagcct gattcgtttg taaaatcagg caaatttttg caaaggcgga
     6541 tggtcagaat ttaaagcaaa ccacagcgac gtttactgcg ttcttccgct tcctggtaaa
     6601 gctggaactc atccaaaacc ggacaaccca acgcctgctc aaatgcttcg cgactggcat
     6661 taccgtgact gcgtgtctgc gtttcattac ccagatttga ctggaagatc cccgccgcgc
     6721 taacgggcaa gaaatcttca taggtaatgg gttgcgccgc gacccaacca cgttcaatta
     6781 agggctgtgg atcgtctccg ggatgaatcg cctgacgatg cgcctcacct gaaggcgtca
     6841 gacggtaccg gaaccatgcc agcccttgct gacgcattaa aaactcactg tcaggaaaag
     6901 tgcggaaggt ttcctgtaaa tgcatttggt gagtgagatt atcctgcccg gttccagcgt
     6961 tacgcagaag atcatcatac agttgtcgcc ctttcggcgt taatgccacg ccacgctgct
     7021 caatttcacc aaagcgcgcg gtatgcgtgc cctgtttctg ccccgcaaac aacaccgtct
     7081 cttccagtgc tttaaagctg gtctggcgta gtaaaatcgg tacctcgcgg cgcggcggcc
     7141 cctcgatgag aattttgggt tcaattccgc attcaggcat catcgactgc acccggtcaa
     7201 tatccagcgt acgtggcgtc aggtggttga tatggcatcc aggaaaacag accacatcag
     7261 caatcaaccg atgttcgttg tgcaaggcgc gataggtttc ttcatctacc gttgctgact
     7321 ggtgccagcg aaacgtttcc agggcttcct gcacaaactc ctgtgcctgt gtttcgttaa
     7381 aaccgccctg ctgctcatat tcctctaaca gttgtcgaca acgtggggtg aagatatcgc
     7441 gctgacgcag aatctccgcc gctttctggc gcaaaatttc gttctcgata agctcaaggc
     7501 ggagtaagga ggtaaaaacg cggaagggat tacgcgccag agaagcatca tcaatgggcc
     7561 gaaatgctgt cgaatgtacc ggcacccctg cctgcgagag atcgtaatag ctcaccgggt
     7621 acatccccat aatggcaaac atccgccgaa gagtagcaag ctcttgtgca gtcccaacgc
     7681 gaatcgcccc atgacgttca acattcagtc gcgccagctc gtctgcgttt accatttttt
     7741 cgtgcagttg aggattgttt tccagcacag ccagattcac atcagctacc agttccagca
     7801 gcgtgccata ttgcggaact tcttgctggt acatggctga cattgcctgc gaaaactgtt
     7861 cccgaatctc atccgccgtg atgctgttcg ccatagtgtc tgcctccagt ggtcagtaat
     7921 ctggagtgta ggtaaccgca ttcactctgg cgggaagaat ttacaaactg tgatctcgcc
     7981 gcgaaaacat caatattatc cattttgctg taacataatt gctttaattg ttaataatat
     8041 tttgcaatca agttatcata atcaaacaac ttcacttgtc agcgacaccg cttcgttttt
     8101 aacatcgctt atggaaaaaa atagtctgtt tagtcagcgc atccgtttgc gccaccttca
     8161 tacattcgta gctgtcgcac aacaaggaac tttggggcgc gcggctgaaa cccttaattt
     8221 gagtcaacct gcgctctcta agacattgaa tgaactggag cagctgactg gcgctcgctt
     8281 gtttgagcgt ggtcgtcagg gggcgcaact taccttaccc ggcgaacaat ttttaacgca
     8341 tgcagtcaga gttcttgacg ccatcaacac tgccggacag tcgcttcatc gtaaagaaga
     8401 tcttaataat gatgtcgtca gggttggtgc actacctact gcggcactgg ggatattacc
     8461 ttcggttata ggtcagtttc atcagcaaca aaaagagacg accttgcaag ttgcgacaat
     8521 gagtaaccct atgattctgg cgggtttaaa aaccggggaa atcgatatcg gcattggtcg
     8581 gatgtcagat cctgaactga tgaccgggct taattacgaa ctgctgtttc ttgaatcgct
     8641 gaagctggtt gtccgcccta atcacccgct acttcaggag aacgtaacgc taagccgagt
     8701 gctggaatgg ccggtcgttg tatcaccaga aggcactgcg ccacgccagc attcagatgc
     8761 attagtacag agccagggat gtaaaattcc ttcgggttgt atcgaaacgc tgtctgcttc
     8821 gctatctcgt caacttacgg ttgaatacga ctacgtgtgg tttgtccctt ctggcgcggt
     8881 aaaagacgac ctgcgtcatg ccacgctggt ggccctgcct gttccgggac atggtgcagg
     8941 cgaaccgatt ggaatactga cccgcgtaga tgcgacgttc tcttctggtt gccagttgat
     9001 gattaacgct attcgaaaat caatgccgtt ctgaaaggtg aagggatctg tcgatccctc
     9061 cttgaacatt ttcacaccgt agcgaaacta actggttcac ccgctccgcg aggttctgcc
     9121 gacacagaat gtttgtgcag acggaataca tccaccgcct cagttaatcg tgccgcctgt
     9181 tcttcaaggg acaccgccgc cgctgaggcc tcttctacca gagaagcatt ctgttgcgtc
     9241 accttatcca tttcagaaat cgcctggcta acctgcgtta tgcctctact ttgttcatcc
     9301 gaggcggcgg cgatttcctg catgatatgt gtgacactcg cgacggcatc aacaatagtg
     9361 ctcatggttt tccctgccgt tgccacctca tccgacccca ggtcaattaa cctgactgat
     9421 tcactgatca agccttcaat ctctttcgcc gcttgggcgc tgcggcttgc gagtgtccgt
     9481 acttcgctgg cgacaacggc aaatccacgg ccttgctcac ccgctcgcgc ggcttcaacg
     9541 gcagcattca gtgccagaat attcgtctgg aaagcaatac tgttgatgac ggcggtgatc
     9601 tcagaaattt tcttcgaact cgtggagata gcgcccatcg tttttactac accggaaacc
     9661 gtctgcccgc catcgctggc tttaatagaa gcctcttgcg ccagtttgct ggcatgatgc
     9721 gcgttatccg cattctgttt caccgtcgca gtgagttgct ccatgctggc ggcagtttgt
     9781 tcgattgccg ccgcttgttc ttcggtgcga gatgacaggt ccgcattgcc agctgaaatt
     9841 tcgctggtgc cacgataaat ctcttccgca ccctgtcgaa cagtccctac tgtcatcccc
     9901 agtgaatgct gcatttgctg taaatgacga cttaagcgac cgatttcatt acgacccgcc
     9961 ggttcatcat tcatcgtcag atcgccactg gcgatttttt caatccgttg tgcggcatgt
    10021 tgcagtggac gaatgacgat ccgacgtagc accataaatg ttatcagcgt catgaccagg
    10081 gcaagcacaa acgcgccaat catgaacatc ccacccaggc gggtgcgctg atgggccagt
    10141 tccgccagtt ggttggctct ggtgctacgt atcttaacgg ctttgttcaa aatatcggta
    10201 taagcattat ccagcggtcg gatctgctca ctttcatgat tgataatcgc ttcaaacatg
    10261 ccatttttgg catatttcaa cataggttgc atacccgtga tataagcctg aaagcgttga
    10321 tttaattcag tgtcgagggc ttcatcagca ggtgttttca ccggccgatt ctgataagca
    10381 cgataccctt gctgcgactg tttaatctcc gattcggctt gcgcaatatt tcgcttcatt
    10441 gcttccattt ccgcaatacg actcgcagcc ccggcctgaa tcatattgat ccgggctgaa
    10501 cgcaaaaaat ctgaactgtt cgctaacccg gtccgaatct caatctccgc agtaacatca
    10561 cgctgatcgc gatcggcctg ccagaggaaa tagccagcca gagcagaact taatgcgaat
    10621 agaactaaga taccgccaag aatgcaggca aataacggaa ccagcctgat gtgatgcaaa
    10681 aaacctaatc gctgtgaggg agttgtattc attaccgttg tctctcgtcc aggtttactc
    10741 ctgatagaaa gtcatcggca cttgacggta attacttatg accaaaaacc tctttgcgtc
    10801 acatttttac aacaattatt gcagagaaaa ttaatgctat tggaatgaag agagtaacga
    10861 atggctaaac tggctaatcc gaatgacgaa aaattctgga aaacagcacg caatttcgtg
    10921 aagtgattat tttcgtccct aaggaaacct cataggatgc ctgcgggtgc ctgagacttt
    10981 ctgttttcag gctaaccggg aatcagaaag acgaaggccc cgaccgatat ttctatcaac
    11041 cgaggccaac gaatgaacct agcaattcag atcttagcct cttacccgcc atccggcaag
    11101 gagaaaggct atgaagcaca accctctggt ggtgtgtctg ctcattatct gcattacgat
    11161 tctgacattc acactcctga cccgacaaac gctctacgaa ctgcggttcc gggacggtga
    11221 taaggaggtt gctgcgctca tggcctgcac gtccaggtaa gggcaagcgc ggggattttc
    11281 cccgcgcatc tgactagcag attcattttc tcatggcacc ccttttacac ttcagttagt
    11341 gcatgttttt ttcgaggcac taatagaacc atcattacag acaaatttgc cgcctgccgt
    11401 acagtgtgag atcccccctt tcgaaccaga acagggtttg cgcccggcat cggcataaaa
    11461 ggaaacaagc gttcccataa acaaaataag tgccagtttt ttcatataac ctccatggtt
    11521 tatcgaatct tatgttcgat ttgaacgtta tacgctggtt tattttgata ccgttgatga
    11581 gatcaaagat cgggtgaatt gattaaaaat aagccagatg gacgtatggg caaggattat
    11641 ggttttattt gtcatacaaa taaatataat aggcgcttcc gatgtagacc cgtattcttc
    11701 gcctgtacca cgggtcggtt ttagtacagg cgttttcttt aaatatcctt caggaacgtt
    11761 tacgcggcat catgcgtaga agtgtgttgt ccttccagaa atagtggtgt gccagtgcag
    11821 ccgcagcgtg caacccgatg acaaaatatc ccagattcgc cagcgtctca tgccacgact
    11881 ttaagctatc aacccgttcg aaattggcct ctgaagcgta aggcatcgtc aaaccaaacg
    11941 caaaccacgg gttgccccgg ttatacatca tcaccaaacc aatcactggc agcgcaataa
    12001 acaggagata aatcaccaaa tgtcccaaat gcgccagtcc cgtcatcatc ggttttggct
    12061 taggtataat cggcggggtt gggtatttca gtcttaacaa aagacgaacc accatcagca
    12121 cgagaattga gataccacag gaaacatgaa ccatgttgat gagtggccga tcgctacgtg
    12181 ggaagaaacc acgaaactcc attgcgcaat acgctgcgat aaccagcaaa aagaccagcc
    12241 agtgaatgct gatttgtaac cttgagtatt tattttccat aacatttcct gctttaacat
    12301 aattttccgt taacataacg ggcttttctc aaaatttcat taaatattgt tcacccgttt
    12361 tcagtacgtt acgtttcact gtaagaatgg agattgccca tcccacaatc ctgggctaag
    12421 cctggaaagg atcaattttc atccgaacgt tcctgacagg agaaaaccat gagtaaagtt
    12481 ggtattaacg gttttggtcg tatcggtcga ctggtgttgc gtcgattact tgaagtcaaa
    12541 agcaacatag acgttgtcgc tattaatgat ctcacttccc caaaaattct cgcctatctg
    12601 ctgaaacatg attcaaacta tggtccgttc ccctggagcg ttgattttac ggaagattca
    12661 cttatcgttg atgggaaaag tatcgcggtt tacgccgaaa aagaggctaa aaatattccg
    12721 tggaaagcga aaggggcaga aatcattgtc gaatgtactg gcttttatac ctccgccgag
    12781 aaatcgcagg cgcatcttga tgctggtgcg aagaaggtgt tgatttccgc ccctgccggt
    12841 gaaatgaaaa ctatcgttta taacgtcaat gacgacactc tggatggcaa cgacaccatt
    12901 gtttccgtgg cgtcatgcac cactaactgt cttgcgccga tggccaaagc cttgcatgac
    12961 agtttcggga tagaagtcgg cacgatgacg accattcatg cctatactgg cacccagtca
    13021 ctggtggatg gcccacgtgg taaagattta cgtgcttcac gcgcagcggc agaaaatatc
    13081 attccccaca ctacgggggc ggcaaaagcc attggtctgg tgatcccgga actgagcggc
    13141 aaactgaaag gtcatgcgca acgcgtgccg gtgaaaacag gttcggtcac tgaactggta
    13201 tcgattctcg gaaaaaaagt gactgccgaa gaggtgaata acgcacttaa acaagcaacc
    13261 accaataacg agtcatttgg ttataccgat gaagaaatag tctcttccga tatcattggc
    13321 agccatttcg gttcggtgtt tgatgccacg caaacggaaa ttaccgccgt gggcgattta
    13381 caactggtga aaacggtcgc ctggtacgat aacgaatatg gcttcgtcac gcagcttatt
    13441 cgcaccctcg aaaaattcgc taaactctga cgcgcacagg cggaggaaaa aacctccgcc
    13501 tcttttactc attacgactg taaataaacc acctgggtct gcagatattc atgcaggcca
    13561 tgtttaccat ctgcgccgcc aataccggat ttacgccatc cggcgtggaa gccttgcata
    13621 gcttcgaagt tttcacggtt gatgtaagtt tcaccaaact tcagcccttt aatggctttc
    13681 atcgcgacgt tcagattttg ggtatagatt gatgaggtca ggccgtaatc actgtcatta
    13741 gccattgaga tagcctcttc cagcgtgtca aatgcgacca ccggcagaac cgggccaaag
    13801 gtttcctcat gcataatcga catttcctgg cgaacatcca gcagcaatgt cggcggataa
    13861 taatatcctt tcccctctac cgctttgcca cccaacgcca ctctcgcccc ttcttctact
    13921 gcgcgcgcca ctttttgctc aaccctttcc agcgccgcgg cgttaatcaa cggccccatc
    13981 gcaatgtcgt tgcgttcagc ggggttacca aattgaaccg cctgcatcgc ttcacccagc
    14041 cgattgacga actgatcata aatgcctttc tgtacataaa cacgttctgc acagttacac
    14101 acttgcccac tattaatgac gcgtgaatca acgatggctt tgactgccag ttcaagatcg
    14161 gcatcgtcca ttacgatagc tggtgcttta ccccccaatt ccagacacac tttggtgatg
    14221 tttttcgccg cagtcgccat gatcttctca cctgcagaga cgctgcctgt catactgacc
    14281 attgcgacct ttgggttacc cgccagttct tgcccaacgg tttcaccacg ccccagtaca
    14341 aggttaaaca cgccgcgcgg aaggcctatt tcatcgacga ttttggcgaa tgcaatcgca
    14401 ttgtttggcg taaattcact aggtttaatg acgatggtat taccggtcaa aagagcggga
    14461 gccattttgc gggcaatgag gaagaacggg aagttccacg gcagaatgcc ggtagtcaca
    14521 ccaagcgcac gtttaaacaa aagaatattt tctcctggac gatcgctttg aataatctcg
    14581 ccctcgtaac gccgtgccca ctccgccatg taatcgatat agtcggcagt aaaagccact
    14641 tcgacttcag ccagctgctg gatcttgccc ccttcttcaa caatcagcgc actgatttca
    14701 ctggcgcgtt cgcggatccc ggcggagatt ttgcgcaacc aactggcgcg ttcaatagca
    14761 ggcaacgctt cccattctgg ttgtgcacgt tctgctgcat cgattgcctt acgggcatcc
    14821 tcggcctgac catcgggtat gcgggaaatg acagcctctg tagcagggtt taccacatca
    14881 atccatgcgt ctccacgcca ggtaacaaac tgtccatcga tatacatagg atgttgaacg
    14941 ggtactgaca tgggcgactc ctgtgattta tatgtttatt ttcggtgaat gcattgttaa
    15001 ttaacatcaa tcatcagaga gacggcatcg tgaaaggaca attctgtgaa gggcttcata
    15061 aaaacaagtg taaaaggaat aaaaggcaga aataaaagtc aggagtcatg cccgtttgca
    15121 aacaagtggt tcacaaacgg gcaattttta acgtaacgag cgactttcca tcgcctcgat
    15181 gagcaccgca tcatgtttca gcgtttgcca tgcatggatg acttctgccg gaaaatcaac
    15241 gtgaacgata aaatctcgcc cgcggggacc gtagccatcg ctatcatcgc gtaaacgcgg
    15301 cagctcgcca gtgagtagtg agagataacg ctcaaccggc cataatcctt gtaactgatt
    15361 aataaagatt acactgtctg cgttatttcc taactgagga acgaatccgg gataacttaa
    15421 ccagcgtggt gcatcgggat tgtccccagt catacggcgg aacgtcgcca tcgtgcgccg
    15481 ctgcatggtg gggtcctgaa caacgatagc cgtatgaact cgttctacgg cctgtttcaa
    15541 tagcgcgatg ctaaagcgtg cgttttcacc gcagtttgtt gactggtctt caatccagat
    15601 tttttcatgc ggaatgtgcc agaactgatg agcgatatcc gccaggatgg tcgcttctgc
    15661 tctgccagtg gtgcggatag tgttgtagtg cggatgctgt gcgatggcgc tatacaaaaa
    15721 agttgtcgag tgaccgatac caccactaat cagtaaagga atttgctgat cgcgagcaat
    15781 cttacatgcc gcgtcgatag tcggcataac cgcattgcct gcaaggatca cgcaatcggc
    15841 ctgatacggc acctcaccgg agaaatcatc ctgcgccagc cactgtccga taacatttat
    15901 ggcatctata gttgccggcg aaagcgtcgg aaacggggta atgttcatgg ttcctcctta
    15961 tcgttccctt aagaatactg cgtgagataa tagtgagaag cggagcactc tgaaaggagg
    16021 aagtcgtatg tccttaaccg ccactccgca agcctgacat atcttttaag gaaatgctgt
    16081 agataaacat tattcatctt gcagcagaga attgtatttt tctgaattcc tgaattttta
    16141 cccttaccgt tttagaatta ataaaaaacc cggcgctaaa tgcttaccgt gttttttctt
    16201 tatcaggcaa atagcagggt taaccgctaa tctgctccat cgcctgcaaa atacgcttat
    16261 ctgaaatcgg ataaggcgta ccaagttgtt gagcgaagta actaacgcgc aactcttcta
    16321 tcatccaacg gatctctttc acgtcttcat cctcacgacg tgcaggcggc agtttgttga
    16381 tccattgctg ccacgcctgc tggacgtttt cgactttcag catctgagca cggtcgcgat
    16441 gtggatcaac cgccagtttt tccagccgtt tttcaatcgc ctgcaaatat cgcagcgtgt
    16501 cgcccagccg tttgaagccg ttaccagtga caaatccgcg atataccaac ccgcccatct
    16561 gcgctttaat gtcagaaagc cccagtgcca tggtcatatc cacccgccct ttcagacgtt
    16621 tgttgatatt gaacactgcc gtaagaattt gctcgacctg cttcgcaata tctaccaccg
    16681 tgtcgttcag ttcggcacgc actttctcat gcagcgcagc aaagccttct tccgtccaga
    16741 ccgggccacc attggcgtcg atcagttgat ccacaccgca ggagatacag tcgtcgatca
    16801 gctccagcac tttgccatac gggttaaagt acagtccaag cttggctttg tttggtaact
    16861 tctcatgtaa atacttgatc ggcgagggaa tatttagcag cagtagacgg cgaagaccgt
    16921 tccacattgc ctgtttttgc tccagcgggt tatcaaacag tttgatcgcc acactgtcac
    16981 gttcatccac cagcgccggc cacgccttca ctttatagtt gccgcgcttc tgttcgtagc
    17041 tttccggcag ttgcccaaag ctccagatat gcagcccgct ctgctcgata ccgtcatccg
    17101 ccaccgcgga cagcgtttcc tgcactttgc ctttcagcgc atctttcaga tcctgtaatg
    17161 agcgcccttc ttttagcttc ttatttttgt cgtccaccac gcggaaggtg attttcaggt
    17221 gatcgggcac ctgatcccag tgccagtctt cgcggtcaac ggtaacaccg gtcatccgcc
    17281 gtaactcgcg ctcaaggctg tcgagcaacg gtaactccag cggtgtgacg cggcctaaaa
    17341 acgcttcggc gtagtttggt gcgggtacaa aattacggcg taccggtttc ggcaacgatt
    17401 taatcagagc aatcaccagt tcgcggcgca gaccggggat ctgccattca aacccgcttt
    17461 cctcaacctg gttaagtaac ggcagcggaa tatgtacggt cacaccgtca gcatccgcgc
    17521 cgggctcaaa ctgatagctc aaacgcagct tgagattgcc ctgatgccag aagttcgggt
    17581 aatccagctt gctgattttt tctgcgccct ctttgatcaa catgcttttt tcaaagttga
    17641 gcaaatcagg ggtttcgcgg ctgacttttt tccaccagct gtcgaagtgg cgtgcagaga
    17701 tcacatcgtg gctgatgcgc tggtcgtaga actcaaacaa cgtttcgtca tcaaccagaa
    17761 tatcgcggcg acgtgatttg tgttccagct cttccacttc ggcccgcagt ttcaggtttt
    17821 cacggaagaa tgcgtgacgc gtctgccagt caccttccac cagcgcatgg cgaataaaga
    17881 gttcacggca taacgccgga tcgatctggc tgtagttgac cttacgcgcg gcaacaatcg
    17941 gcaacccgta aacggtgact ttttccgttg ccatcaccgc gccctgcgcc cgttcccagt
    18001 gcggttcgct gtaggtgcgt ttaatcaaat gctgagcgac aggctccacc cattccgggt
    18061 cgatacgcgc agcaatgcgc ccccacaggc ggctggtttc taccagttcc gccaccatta
    18121 cccatttcgg cggttttttg aataaaccag aaccggggaa gatggagaaa cgcgcgttac
    18181 gtgcgccggt atattcttgt ttatcggcat ctttcatgcc gatatgggaa agcaaacccg
    18241 tcagcaacgc aatgtgaatt tcgcgatatt ccgccggttc gctgttgacc ggaatgccaa
    18301 gttctttcac cacctggcgc aactgggtgt agatatcctg ccattcgcgc acgcgcagat
    18361 agttgagata atcggtacga cacagtcgac ggaaggcgtt agaagaaagc gccttttgct
    18421 gctcgccaag ataattccac agatttacaa acgcgaggaa atcggattct ttgtcgtgga
    18481 agcgacgatg tttttcgtcc gatgcctgct gtttatccat cggacgctcg cgcggatcct
    18541 gaatggagag cgcggacgtg ataatcatcg cctcacgcac gcagccatgt ttttgcgctt
    18601 ccagcaccat acgcgccaga cgtgggtcga caggcaactg agagagctgg cgaccgagcg
    18661 ccgtcagttt ataggcgctg gcctgttcat ccgtggtgat cgcgcccagt tcttcgagca
    18721 gacgcacgcc atcctggata ttgcgtttat ccggtgcttc gacaaacggg aacgcagcga
    18781 tatcgcccag ccccagcgcg gtcatctgca aaataaccga ggccaggttg gtacgcagaa
    18841 tctccggatc ggtaaactcc gggcgcgaga ggaaatcgtc ttcggaatag agacgaatac
    18901 agatcccttc ggacacacga ccacagcggc ctttacgctg attggcagac gcctgcgaaa
    18961 tgggttcaat cggcaaacgc tgcactttgg tgcgatagct gtagcggctg atacgcgctg
    19021 taccggggtc gatgacgtat ttaatccccg gtacggtcag cgaggtttcc gcgacgttgg
    19081 tcgccagcac aatgcgccgt ccgctgtgcg actggaacac gcggttctgt tcgctgttcg
    19141 aaagccgcgc ataaagcggc aagatttcgg tatggcgtaa gttcagcttg ttcagcgcat
    19201 cggcggtatc gcgaatttcc cgctcgccgc tcataaagat cagaatgtcg ccggggcttt
    19261 cctggcttag ttcgtctacg gcgtcaaaaa tcgcctgcaa ctgatcgcgc tcggtgtcat
    19321 cggcttcttc aacaatcggg cgatagcgca cttccaccgg ataggtccta ccggagactt
    19381 caataatcgg cgcattatta aagtggcgcg aaaagcgttc cgggtcgata gtcgcggaag
    19441 tgataatgat ttttaggtca ggacgccgcg gcagcaactc tttcaaatag ccgagcagaa
    19501 aatcgatatt caggctgcgt tcgtgcgctt cgtcaataat gatggtgtcg tactgcatca
    19561 gcaggcggtc ttgctggatc tccgccagca ggataccgtc ggtcatcagc ttgaccatcg
    19621 tgttatcact tacgtgatcg ctgaaacgca ctttgtaacc gatgcaaccg cccggctccg
    19681 ttttcagctc ttctgcaata cggttcgcca ccgttcttgc cgccaggcga cgcggctggg
    19741 tatggccgat cagtccttta atcccgcgcc ccagctccat acagattttc ggcagctgag
    19801 tcgttttacc agaacccgtt tccccggcga cgatcactac ctggtgatca cgaattgctt
    19861 cgagaatgtc ctgctttttc tgactaaccg gtaaattgtc aggataagta atttccggtc
    19921 gtgccgcttc acgcagcagg actttccctg ccgcctggtc aatctctttc gccatctcct
    19981 ggaaaatggc ctgttgtgca tcaggatttt taaccttctt cacaccgtgc agacggcgag
    20041 aaaaacgcag tctgtcgcgc agcatcagcg aatccagccg ctgctgcaag gccgtaaagg
    20101 tcaatttttg ttgttctgtc ataacgttag tgggcagtag tctgcctgat taattttctt
    20161 ttaaatgata gatatagagt accacatcgc agctttctat gcgttgttca ataaattcga
    20221 acataggctt cgatttattg cgctatctct gtggcaggat tgtgaataaa gtgtcagcaa
    20281 gcaacggggc atcgcccatt caaacatcta taaggaaaca ccatgagcaa ggtattagtt
    20341 cttaaatcca gcatcctggc agggtactct cagtctaatc aattgtccga ttattttgtt
    20401 gaacaatggc gcgaaaagca ctccgctgat gaaatcaccg ttcgcgacct ggctgcaaat
    20461 ccgattccgg tactggatgg cgaactggtt ggcgctctgc gtccgagcga tgcgccgctg
    20521 actccgcgtc agcaggaagc tctggcgctt tccgatgagc tgattgccga gctgaaagcc
    20581 cacgacgtta tcgttattgc ggcaccgatg tataacttca acatctcaac tcagttgaaa
    20641 aactattttg acctggttgc acgcgcaggc gttacttttc gctataccga gaacggtccg
    20701 gaaggtctga taacgggtaa aaaagccatc gttattacca gccgcggtgg gatccacaaa
    20761 gatggaccaa cggacctggt gacgccgtat ctgtccacgt tcctcggctt tatcggcatt
    20821 accgatgtga aatttgtctt cgccgaaggg atcgcatacg gtccggaaat ggcagcgaaa
    20881 gcgcagtctg acgcgaaagc ggccatcgac agcattgttg ctgcataacc ggacacgatc
    20941 ttgatgtgga ctgtagttca gtccacatcc caatccactt atctgttttc ccataatttc
    21001 agcatcgctt tgtgctcgtc tgtcagcaca atctgcgggc gtcttgctcg aatatagcta
    21061 atcgcttcat taacggtttt acagtgtccg taacataaca accatgccgc caccaccagc
    21121 gcactgcgcg ataatcccaa tgcacaatgg accagaacgc tgccttgctc ttcgcgtaat
    21181 gtttccagca tcgccacggc ctgtcggagc tccccctctt ccggaaccac cagatccagc
    21241 atcggtacac aaaaatagag tcgatctttt gtggctcgtc cgcgagggaa ttcaaaggtg
    21301 acgtccagaa ccgcattctg tgccggaata tgtcgtggaa acgcccctaa ataaacacca
    21361 gcagtaattt tgctcaccgg ctccaggcga cgacaaaacc aacgcataga tagccacatc
    21421 ccaatgcgcc agggcaatgt cagccagtaa acggcgggcg ggagtttccc ctgactatct
    21481 ttccctgttg ttatcgcgcc aagcccaccg tacccccggc caataatgag tagcgataat
    21541 actggccaac ataaccagac tgaccaccat aactgaatca tcaccattag ctccatcaac
    21601 acaatgcacg agcccgcgcc tacgacatat ggcagtgcta ttttgattcg acgttgatca
    21661 ggtttctgat aattccaacg acgatcgacg ggcaccatcc agtcaatcaa catacctacc
    21721 gccagccctg tgatgacatc aataaaatga tgctgccagg tcgtcagagt agaaatagcg
    21781 atgagtaaaa accatccgcc acagacttta cgccacctct cagccagatg ctgacgaaag
    21841 tgacgccaaa gtagccagca gagaataata tgcagcgaag gagactggtt ataaggcaga
    21901 tcaaacagtt caagttgcga aaatagccat cccgtcaccc cactcacttc aggacggata
    21961 aaactaaact tcagcggata gagcaaaaaa ccgcagcagg ccattaccgt tgccagaata
    22021 agccggtgga caagtcggcg ctgttcgaat gtcgtgctac aaacgaacag cgaaaatcca
    22081 tataaaagat ccagactcca gtaaggaaca atagtccagg gaagaaaagg gatcgccgtt
    22141 tcccaaccga atacctgact ggggatatca tggctgttaa ggtcctgaac cgcggtgaac
    22201 tgattaagag atccataggt gaagaagaaa aacggggcca gcaacaataa ccagccagcg
    22261 ccttgtagca atacgttccg acgttcagtt atcacgacgc accgccatcg aaaccgtaaa
    22321 aataccccac tcatcaatcc gttgtgtgca tttatcaaat ccggcatcac gcacgagtga
    22381 atccatctcc ccttgcgaac gcacgcgcat tacccacggt ttaccatctt tatgactggt
    22441 taacaccccg gcaatcatct ccagttgagg gtgccacggc tgcccggtgt agatgagaat
    22501 gccgcccggt tcgatggcat tggcaagacc tgcgagtgag ttttttacct gctcattttc
    22561 gggaaaaagc tcatacaggc cagagacaat cgccagcgta ggccgcggag ttaacgcgct
    22621 gagttcctcc gggttaaacg catcgccctg ttcaaaacgc acccgcccag acattccccg
    22681 ttgggcaatc atcgcttgcc cctgtgcaac atttaactca ctgtaatcac gtaacaaaat
    22741 atcgcttacg gcaggctcgt ttgccagcgc atccagtaca tagcgcccat gccctgcggc
    22801 aatgtcaacc acgcggacgg ctaaaccttt ggcgtggaga tcggcaacgg cttgtttaat
    22861 cagtatttgt aaatgggttt tacgctggcg aataccgcgc cagccaacac tgttcaggta
    22921 gattttgtca attaagcgcc cgaatgcgtt actgccttgc ggttgatttt gatagacata
    22981 atccagggaa ctgcctgaat caaagccggt gctcattccg agatgcaggc ccgcagagtg
    23041 cgccccgaac aatttcatcg cctttcgcat aaagcgatac gccaaatcaa ccggcgataa
    23101 tggcacgggt ccacctgaaa ggagccgcca gcgatccgct gatggtccag tgcggtcttc
    23161 atgctgataa tcaaattttt gtgacttgtt agcatataaa cgactaataa agctttgcat
    23221 tttttcaaat gcctgcgccc tgttctcttc acccaacgtg tcgtgataaa agcctggcag
    23281 cagatgcagc tctttcagag ggctacgtaa tctctgataa aaatcaatct gcggttggcg
    23341 atgcaccaca tagtcatcgc ctgatatcag aagttgcgtg gggagcgtaa tcgccgccgc
    23401 atctctaata atacgttcag acgttttgta gagatcgagc aagatattaa cggcaatcgc
    23461 ccgtgtgatc agcggatcat tattgaaact cgccacccgt tgccgatcgt gggtcaaata
    23521 gcgtcctttc acataggaat taataaaaaa cagaccacgc agacgatgcc ataacgccag
    23581 cgcaggacgt gccagcggta catacaattt aaccttaaag gccggagaag ccagcaccag
    23641 cccgcgaatt gcaggcgcat aatcatgaac ccatgtggca accagcactg cgccgacgct
    23701 ttgcgcgatc actaccacct catccagtcc gacctggcta tcgctggcag caaaacgaac
    23761 aaattcatcg acatcctgca ctgaacgcgc aagagatggg ctataaccac gcggccccga
    23821 agtttgtcca tgccctcggg catcccaggc ataaaaaaca gtatctggca tcgccagttc
    23881 atcaacgaga tgttgtagac gaccagaatg ttcatgaccg cgatgaaata agacgatgac
    23941 ctttttagcc ccgggctgta aagcgggcca gtgccgataa aacaacgctg tattatcact
    24001 gctggtaaaa aaatgttccc catggatgcg tgaattttcc attttctcta ttttccttca
    24061 gtaacagcag tagcgaataa aataaaaaaa taccggagcg gtgaaaatta atgagtcaat
    24121 cctgtcaagg agtccgccat gtcctggtaa tagtttccca ctatctttaa caccaatatc
    24181 tcgtttgatg gctgacatca cgacatcgcc gcaaaaacca ctaataccaa ttaacaaacc
    24241 cgctaataat gcctgtaatg tatttagcgg tgtcagcaac ggtccaataa tcagtgacgc
    24301 aatcatggtg gtgattacgc cccccaccag accttccagc gtttttcccg ggctgacttt
    24361 ggggaccact tttcttctgc cgcaggattt cccccataaa tactgtgcaa tatcgtttga
    24421 ctcggttaat gccaaaagaa ataacaccag taacgccccg ccctggatat ttgtggttgg
    24481 caacattaat aaccaggcgg catgactcaa ggcgaaaacg gtcattatcc agccccaaaa
    24541 aatggcgctc acggtatgca gaaaaccgga ggggtccccc ataaaaactt gcccggtggc
    24601 taatattaga aacccagcca aaggtataaa taatagatac agctcaaagc aattaaaacc
    24661 gataaacaaa tagttaagag gaataaccca atataaccac cgcggaaaat gtacagatat
    24721 aagcgtacaa tattctttca atgccagaaa acttatcagc gcaaaaaacg tcaacgtcat
    24781 ccagcgtgga cctgaaatca ccagtgaaaa acaaatgacg atgccccacc aggtacgaat
    24841 acgtaatgta acttcatcct cttttctctc cggtaaccgc catatcagaa aatggtttat
    24901 cagcgttgct aaaattaaca acgcgaaaag tgttgccaga gatttttcca gcattatctt
    24961 tcagcgcgca taagaagcac actccgacaa cgattaatcg cagtccagag aagcagaatt
    25021 gaagcaatgc tccataaaag attattccac tgcatccact gtggataaat ggcaacggcc
    25081 agaccccaca gaccaaatat taacgcgcga tcgcttttgc caaatggacc ggcataactg
    25141 cgaacaccgt taatcgtctg cgcgagtaaa ccgcaaaact cggtcaatat ggtgcaaaat
    25201 aacatgagta tcacgagtga tgcgttactt tccggtaaaa ataaaaatgg taaatagagc
    25261 gcaatatcgg aaataacatc accagtctca ttcaaaattg ccccaagccg tgtttgctga
    25321 ttgcactcac gcgccaacat gccatccagc gcattgagcg ccatacggat aaaaagcacg
    25381 atgggcaata gcataaagag gatgggttgt gccaccaaca tcaacagcaa tccggtaaga
    25441 agagaaagcg ccagtgcagc aagagtgatg tgattcgctg taacgtggtg cttgtaaagc
    25501 caaaacatcg tcggccttaa cagcgactga aagagcggtt ttatctggta gagcgtcatt
    25561 atcaatcctt gataattcgg tttgcgctat ggtgcatcaa ttcccctgac ggagatatcg
    25621 caacacgccg cttcattaaa aattcagaat atatcaaggt attggggact cactgtcagg
    25681 aataattatc cccaattaat caaagggtta agaaatatta cgttgtggat caatacaact
    25741 catcaagttt tctcatcgct tgttcccgta ttgcgcgcgt cttaccgtta agcgccagct
    25801 ggcggactaa atccagttca gggttaacaa gctctaacgc cagcaaagga tcacgtttca
    25861 gcctcgcctt taatacatca actggaaaac ggggattatt catcgccaca atccaccact
    25921 cgggatcaat ttctgccgcc agtactgcag gcggtgtgtg gcggttgtta agtgcgccga
    25981 acttgaaaaa tgtgagattg cttttcgcca gtgtactcca ccatatacga tccggttgtt
    26041 ttgccagtac atccagttgt gctaacgcat cggggtcgag tgcataatag acgcgtaatg
    26101 cttcatcaac ctgcgcacgg gagctgtttt cctgcctcag aaaaaacttc gcccaaatat
    26161 cattgcgtgg cgtattttgc tgtaattcct tgaggacaac aacgggtaaa ttatcccaac
    26221 taactaatgc actctctaaa aagtcccagt cttccaaacg tatcagggct gtggcattta
    26281 atagcatctg atcatcaact tcaccgtggc tgataagctc agacaaaatc atatcagcgc
    26341 tggtttcata cgcagctgaa tctgctttcg gattattttt ccgatgcgtt aaagcacgtt
    26401 gccaaatttt ttgtttcatc gacgtgctca gatactgatt tttcgccaga ttactccacc
    26461 cctcaggatc gttgaaacgt gtcaataacc gttgtagagc agcgtcagag tgaattacct
    26521 gcatatcccg acagcaaaag taggcagggg attttttctc catcctttta acaaaattaa
    26581 actgtaagtt atctggcaat gctaaaaata acacacgtat tagatcatcg tcattctcga
    26641 atttttggta taaataggct gcaactttgt cactctcttg tggcgtcatc cgcaataatt
    26701 gaccatggtg tttcatctct gccagcttga acgctaagct tgtgacaaca ttgtttgatg
    26761 aatcgttcgc caactgcatt gcctgattgt aggtgagttg tcctccagca ccgacctgac
    26821 gaatagagga atcatcactg cggatatagc catcaataat ggattcaggc aataaccacg
    26881 catctttata aagctcctga atgatcatat cccaatctct tttcgccaga gtcatgacga
    26941 tatccagagg gagattttca cgtagcgcaa gattcaacgg accgacacca tcaggtagac
    27001 gactcattcg cgtcattatt tcaacagaga gcggttcacg gtcgagcaac cccagaacaa
    27061 caggctggta acccaattgc tgatcggtca actccagaat ctcattgtca ggcatatatc
    27121 tcgccatact ttcaatcgtt tgttcatcat tgctattaat aagttgtttg cgtaggttag
    27181 ctgaaattcc tttaagcctt gggagagttc gcattatggc aagatcaaat gtcgcaatat
    27241 ctcgtccttc aaatgcagca acgttgcctt catccagcat ttgcaattcc tgcactaact
    27301 caggagaagg cttagggcga aacagcgctt cttttttcat ctgattacag acattccctg
    27361 ccatgcgaat attgcttcgc ttaaaaattt ctttcagagc atcttcatca tttattagac
    27421 cccaataata tgcatcagga tttattatca tcagcgcttt tttgacattt tcagtgtcat
    27481 ttttgtcact cgcacggacc atgaattcac tatttgtcga agacgcaaca acaggtgcaa
    27541 ttatgtcatt gataaatatc ccacgcgcat taatactccc cttaatcgta gacggattat
    27601 cgtaatatga gtcgataaat accagtccac ttgcatttat cgaaccggta attaacagtg
    27661 gtgaatccgt aactatattc gttgccgtca cgttaccgag aataacccat ggattagaaa
    27721 gagagaaagt ggaaatatta cctttgactg tcaaattgcc aagaacaatc aatttttgat
    27781 tactctctat attaccttct accactaaat cgccacgatg cactttccag tccgatgcag
    27841 ataaaccgaa ggggacaaga ttcacgctac tgttgatagc ttgttcaact tgttgtttat
    27901 cttcagcggt agctgcagaa aaaaatggtt caatattaac tgcatttgca acacatcgaa
    27961 gttccgcctg tactggaaga ctcagcataa acaggggaag taaacctatc cagctgcaaa
    28021 tcctgggctt aaacatatgc actccatgcc attaaaaaag aaacaaaagg gaaatgcatc
    28081 tttccctttt gtatattcgt tattctcgcg aaataccatc caacgtagac aacacttcct
    28141 cagaaagatg caatttttca gcagccatat tctcccgtaa atgcgcaaca gaagatgtcc
    28201 ctgggatcag caaaatattc ggtgaacgtt gtaacagcca cgccagcgcc acctgcatgg
    28261 gtgttgcacc caggctcgca gcaacatccg acagcgtaga ggattgcagc ggtgtaaagc
    28321 ccccgagcgg gaagaacggc acgtaggcaa tgccgtcacg agccaaagca tcaatcattg
    28381 cgtcatcagc acgatgagca atgttgtatt cgttctgcac acagacgatt tcggcaatct
    28441 tgcgcgcctc tgcaacctgc gtcggtgtga cgttgcttag gccaatatgt tttaccaggc
    28501 cttgttgttg catctctgcc agcacggtca ggctggcttc aatcgatcct tccgctgggc
    28561 catgaccatc ccccatcata atgcgcaagt taaccacatc cagtacgtcc aggccgagat
    28621 tacgtagatt atcgtgcacc gctttttgca actctgccgg agaaaaggcg ggcaaccagg
    28681 aagcgtcctc tccacgccgc gcaccaattt tagtgacaat tgtcaggtcg tcagagtaag
    28741 gataaagcgc ttcgcggata atctgattgg tgacgtgcgg accataaaag tcgctggtat
    28801 caatgtgatt gacgcccaat gccagcgcct cgcgcagcac agttatagcg acgtggcgat
    28861 ctcttggggg accaaaaacc ccaggacctg ccagttgcat cgcgccataa ccaagacggt
    28921 taacggattt tgtaccgaga gtaaatgtat tgctgctcat cgcaccctgc cctgtaataa
    28981 aacggattaa gaaaaatcag tatagaaacg aaaagagata aataatgcga gggcgtaaac
    29041 aagattactg gatgagttgc tctattgtcc tgaaaaacac aaaacaaaac gcgtaaaacg
    29101 ccgcatccgg caataaaaaa accgcaacct gtatcagcgt tgcggtttag gtatcacatg
    29161 ctaaaaatta gaatgttcag tatctggacg ttactgtttc ggtcgcagat tctcatcaaa
    29221 taccagacgt ttcctgttcg gttcaacttc ccgcaatggc tcaacttgat gcaacgtctg
    29281 cttacaacga gtcaccagca cctgatactc atgcgtaccc tgctttttcc atgccagctc
    29341 ctgctcactg agttcacgaa tcgctttcgc cgggctgccg acaatcaggt aattggcagg
    29401 catttctgct ttggctttca caaatgcgga tgcgccaaca atgctgttct cgccaatcac
    29461 cgcgccgtcc atcactaccg cgttcattcc cactaatgca ttgcggcgga taatgcaacc
    29521 atgaaggata gcgctatgac caatatgtcc atcttcttcc acgacagtat cctgctcggg
    29581 aaaaccgtgc ataacgcaat tatcctgaat gttcgcgcca tctttcacca cgatacgacc
    29641 aaaatcgcca cgcaggctgg catttggccc aacgtaaacg cccttgccta gaataacatc
    29701 gccgatcaat accgctgtcg gatggacaaa actctcttct ggcacaaccg gagtcagacc
    29761 atctatctgg taaattggca tatcgcctcc tgttcaatat tcaaaccgcc aaaacgttga
    29821 aaatacaggc ttcccggcgc aggcagttca ccgaccgagg tttcaccttt ttcactaacg
    29881 aacgctaaag cagcaggcgc tacgcgctga taaatgttga tacacagctg acgcgccgta
    29941 tgccctgccc agtgcgccgg aagcaactcc tctggcaaca atgggtcttt aaggacgaca
    30001 cggcgataaa aatggatcag taaaagctga atatgaaatg cacgctccgg ggttaactcg
    30061 tctgctgcct cttttaacag cggcaccagc gggcggaatg actgaataaa ggtttcgtac
    30121 atggcatttt gttcagttaa atgccagcac tcttccactc tggcacgcag tgctgcacga
    30181 gaaagcgcca gtggtatttg cgcttcaaaa caaatcacgt tatccgccac ccccgcttca
    30241 tgcaaaagtg tctgtacatc cgccagtttt tgcgacggcg atgccatcag gctgggtgcc
    30301 agtgcgccaa aaccttgcca gatcaactgc tttttgacat cagccagcgt tgatttatct
    30361 aaaccttccg agagcaacag gagccattta ccatcccatg caggttgctc tgcgcgataa
    30421 attttacttt ctgcccgtcg cgtcaggcgc aagcctttat cactgaggct atagaaactg
    30481 cgtcgcccga tgcgggaaac atccagccag ccttctttat taagacgaaa caaagcggtg
    30541 cgcacgaaac gctcaccaaa tcccagccct tccagcaaag cagccagact acccagccag
    30601 atttcaccac cacgatggga aagcgaatca ccatacagag aggagatcaa agatgtgcca
    30661 ctgaccggaa cagcgtttac agcatgttgg ataaaagtaa caagtttact cattcgattc
    30721 agtctgttgt tattgttgtg atttatgaat catagcacag gtcgtatcga cttctggccc
    30781 tggcgcttta cctcaccagg gccagacgac acgtcaggca ccaacaatat tgcgcagatc
    30841 aaacacccga cacgccttgc cttctgaacg cgggatactg ccacagttaa cgatcatcac
    30901 atcggtagag atccccacca tcgacttaat ccgatggcgc agctggtggc acacctggca
    30961 acgctgctca tgcgtcaatg tcaggctgct ttctttcaac tccactttca cagaaagtga
    31021 atcaagatgc ccccggcggt tcacctccag ttggtaatgg ggcgataaat gttcgaattt
    31081 gacaatctct tcttccagtt gtgacgggaa gacatttaca ccgcgaatga tcaacatgtc
    31141 atcgctgcgt ccgctgatgc gatccatgcg gcgcatagtc cgcgccgttc ctggtaacag
    31201 tcgggttaaa tcacgcgtgc ggtaacgaat gaccggcaat gcttctttgg tcagcgtggt
    31261 gaataacagt tcgccatgtt caccatcggc aagcggtgtg ccgtcatgag gattaacaat
    31321 ttcaggatag aaatgatctt cccaaatggt tgggccgtcg gtagtttcca gacactccat
    31381 cgccaccccc ggccccatca cttctgacag gccataaata tccagtgcgg tgatccccag
    31441 acgccgttca atctctttac gcatggcctg tgtccacggc tcagcaccaa acactccaac
    31501 ccgcagcgag caaccgctgg catcaccgcc caactgccgc tccagctctt caatcaggtt
    31561 aaggcaataa gatggcgtaa ccatgatcat atctggttga aaatcgcgga tcagttgcgc
    31621 ttgtttttcc gtctgcccac cagacatcgg gatcaccgta gcgcccagac gttcggcacc
    31681 atagtgcgcg cccagcccac cagtaaacag gccgtaacca taggcaacat gaattttgtc
    31741 tttgggcgtg ccacctgccg cacgcaagga acgcgctaca atattggccc agttatcaat
    31801 atcgttttgc gtatagccga caactgtcgg tttacctgtg gttcccgaag aagcatgaat
    31861 gcgcaccact tgttccatcg gcacggcaaa ggtgtcgaag ggatagttgt cgcgcagatc
    31921 ctgtttggtg gtacagggaa atttacgcag atctgaaagt tccctgaaat catcaggatg
    31981 tacgcctgct gcgtcaaatt tgcgccgata catcgggaca ttttcatacg cgtgtttgag
    32041 cgtccatttc agacgttgtg tttgcaacgc ctgtaactca tcaacagacg cggtttcaat
    32101 cgggtcaagc tttgtattgg ttatcattgt agggtactcg caggttgata tgctcaaaca
    32161 cgctccagaa tcatggcgat gccctgaccg acaccgatgc acatggtgca caatgcgtaa
    32221 cgaccgttac gccgatgcag ctcatggctg gcagccagtg ccaggcgggc accactcatt
    32281 cccaacggat ggcctaaggc gatagcgcct ccgttggggt taacatgtgg ggcatcatca
    32341 ggcagcccca attcgcgtag tacacccaac gcctgggccg cgaacgcttc gttcagttca
    32401 atcacgtcca tatcgtgaat actcagcccc gcgcgttcca gcacccggcg agttgcaggc
    32461 accggaccaa gccccatcag gcgcggttcc accccggcgg ttgccatggc tacgattcgc
    32521 gcccgcggtg tcagtccttg cgctgctgcc atctgttcac tggcaataat caacgcagcg
    32581 gctccgtcat tcaccccgga ggcattgcct gcggtaatca ccccattggc acgaaatggt
    32641 gcttttaacc cacgtaactg ttccagcgtc gtttccgggc gcagatgctc atcatgttgt
    32701 atttctgtta caacgccttt cttgtttttc aacacaaccg gaacaatctc ctcagccaga
    32761 atgcctgagg attgcgcttt tgccgtacgt tgctgactgc gtagcgcaaa actatcttga
    32821 tcttctcgcg atatttttaa cagttcagct acattctctg ccgtttccgg catgctgtca
    32881 gttccaaatt gttgagccat gagcgggttc acaaatcgcc agccaatagt ggtatcaaac
    32941 atctcagcct gacgagaaaa tgcactggtt gccttgccca taacaaacgg tgcccgagac
    33001 attgactcca cgccaccagc gatcagcaaa tcgccatcgc ccgctttaat cgcccgtgcg
    33061 gcgaacccca gtgcgtccag cccggaacca cacaagcggt taatggttgt gccggaaaca
    33121 ctctgcggca gccccgccag taacgtcgcc atccgggcta cgttacggtt gtcttctccc
    33181 gcctgattag cacagccgag gatcacatca tcgatacact ccgcatcgag acgcgggttt
    33241 cgcaccagca gctcccgcaa agggatagca gccagatcgt cagcccgaac acctgataat
    33301 gccccaccat agcggccaat tggcgtacga attccgtcac agataaaggc ttcacgcatc
    33361 aggcttctcc tgtaatggtg ccgccgatgc ggtgagattt accgcgaaac agcgcaaccg
    33421 ttttttgttg ttggttaaca atttcgatgt cgtaaacacc ggtttgcttg ccctgatgac
    33481 gtacctgcgc agtcgcagtt aaggtgtctc cggcaaagcc tggacgtaaa aaatcaatcg
    33541 tgcaggcaga agcgacggct gccagcccct ggctattgca ggcgtaggca aaggcagtat
    33601 cagccagtga aaatagctgc ccgccgtggc aactttgatg accgttaagc atttgtgcag
    33661 tgacggtcat ggtcactaca gcaaagcctt catccattga gataatgtcg atgccaagcg
    33721 ctttggcgca ggcatcgttc tcatacattg catgggcatt ttgccaggcc ttatgactca
    33781 taaccgctct ccagaagcgc ccgttggcgc agcaatgaac atgggcgata gcgttcttcg
    33841 ccatagtgat gctgtagatt ttcaaggagc cttaatattc gctgccagcc aagttgcgct
    33901 ccccaggcaa gtgggccata tggataattc accccaagac gcatggcggt atcgatatcc
    33961 tgttcagagg ccacgccttt ttgaagcgca tcaagggctt cattgatgat cattgctacc
    34021 gttcgccaaa tcagcattcc tgggtaatct gcaatttgca gcactgtttt gccctgctgt
    34081 tgcaggtaat aaatggcctt gcgggtcgct gagtccgggt tcaccgctgc cgcagcaatg
    34141 gtcaccacct tgcccgccat tttgtcgacc actaccaccg ggcgtgccag tcgtattgcc
    34201 agcgcctgtg ccgtctcgcc ttgtgtctca atcaataaaa catcgtcaat ttccgtgaca
    34261 ccgtcacttt tcttttctac tttcattggg ctaaaactgt cgcttaccgc ttccaggcca
    34321 acaactgcct cacgttccgc gcgccagtcg tacacgccca gcccactttt cttgcccaac
    34381 cgtccaccaa tcaccagttc ctgttgcacc agcgaaggta aaaaacgacg ctcctgccag
    34441 aaagcgttaa acaccgaaca ggtgacagca aaattgacgt cctgaccaat cagatcggtt
    34501 aattccagcg gccccatcgg gaaaccagcg ccatcgcgaa gtgcagcgtc aatcacttct
    34561 ggtgcagcaa cctgctcttc cagtgcccgc caggcctcgg aataataagg acgcgccaca
    34621 cggttaacga taaatccagg ggtcgaatga cagcgcacag gctgcttacc ccaactcaac
    34681 gttagttcac acaactgctc aacaacttcc gctgccgttg ccagcccact gaccacctcc
    34741 accaacttca tcaccggtgc cgggttaaaa aaatgcagcc ccgcaacacg ttcaggattt
    34801 tttacctccg cagcaatcgc ggttatagag attgacgaag tgttagtggt caatagcgtt
    34861 tgtggcgggc aaacttccgc cagctgtgca aagagcgctt ttttgacttc cagacgttca
    34921 gacgccgctt caatgaccag gtccgcagct gccagcgcgt gaatatcggt caccgggatc
    34981 aggcgtttca atgtgcgttc acaggtttca gcagtcagtt ttccccgcgt cacgcgtgaa
    35041 tttagccgcg cgtgtatccc gtcgattgcg cgggtcagcg cttcagcaga aatgtcatac
    35101 agtaaaacct gatgtccatg actggcggca acttcagcaa tgccagcccc catggtgccg
    35161 ctcccaatca ctgccacagt ttgcacattt atcatcatgc tatttccccg tgaactgcgg
    35221 tgagcgttta gccaggaacg cactgacacc ttcacgataa tctgcgctcc gtccggcaag
    35281 tcgctgatag tcgcgttcca gatccagttg cgtatcgagc gtattggttt cagcgctatt
    35341 tatcgcttgc ttgataagtc caagaccaaa tgtcggttgt gtcgccagat gccgtgccag
    35401 ctgttgcgcg gtatctgcca gcgtttcatc atcaacaacc tgccagatca tcccccattc
    35461 gtgcgcctgt tcagcactca gttgattccc cagcagtgcc agccccatgg cgcgcgctcg
    35521 cccagcaacg cgtggcagta accaggttcc accgcaatcg ggtactaaac ctaacttact
    35581 aaacgccatg acgaattttg ctgaacgggc agcaataacg atgtcgcatc ccagtgccag
    35641 tgttgcgcca gcccctgccg ccacgccatt gactgcacag atcaccggtt ttggcagttt
    35701 tgccaggcga cgtaccagtg ggttatagaa acgttcaact gacatcccta aatccggtgc
    35761 ggggcctgtg ggatcaacgt tacgatcgtt aagatcctga ccagcacaaa atccgcgccc
    35821 ggcacctgta agtaacaggc aacggatagt gtcgtcgcgc tcgacctgtt tcaggcactc
    35881 tgccagttgt gcgtgcatct catcattaaa actgttcagg cgttccgggc ggttgagcgt
    35941 tagtgtcatc acgccctttt ctacatgact gaggatgaat tccatcatta gcgtccttta
    36001 aagtcgggcg tgcgtttttg caagaaagcg gagatgcctt catgacgatc ttctgttgcc
    36061 gccagcaagg tgaataactg tcgctcctgg gcaagtccgg cttgcaaagc cacttcctgc
    36121 gactggcgca gcgcttgctt tgccgcttgt aaggccagcg gcgagtgacg tgccattttc
    36181 gatgccagct gtaaggcgta ttcgagggtt aaatcgctgg ggaagacgtc gctaaccagc
    36241 ccggcctgct gtgcttgctg agcggtgata ctttctccgc tcagcaccat tttgctggct
    36301 aacgatttac cgacactacg gattaaacgt tgcgttcctc ctgcgcctgg catgatgccg
    36361 agagtgattt ccggcaaacc aaaacgcgcg ttctctccgg caaccaccac atcgcacaac
    36421 aatgccagtt cgcaacccgc accaagcgcg taaccgttga cggctgcgat gagaggtttg
    36481 ttgaaggcct gcaatcgcgc ccatagctgc gggcgtgtat cgtttaaggt ggccgcgaga
    36541 tctttttctg ccatttcgtt gagatcggcc ccagcggcaa aaaagcgtgc attaccggta
    36601 atcacacaga ccgaaatgct gctatcggta gccgcagctt ccagttcatt taccagttgc
    36661 gtcagaaggg cattatttag cgcattacgt gcggcgggac ggttaagggt cagcaacaat
    36721 acttgttgct gacggctgac gatcagttcg ctcatgccat ccccttcgcg tcaaagtcaa
    36781 ccaccacatc gctggtcagc ggcagtgcct ggcaactcaa cacatagcct gcggccagtt
    36841 catccggttc cagactgtaa ttggtttcca tcgccacttt gccacgcagc actttgcatt
    36901 tgcaggtcgc acagacgccg cctttgcagg catagggcag atcagccccc tggcgcaatg
    36961 ccgcatcgag aatgctttca tcgtcggcat taagcacgat ttcccgatcc cgcccatcct
    37021 gacgtacagt cactttttgt ccgtcacttt gcacgttaac gctacgtttg acgcgcgtgc
    37081 caggcgtatt aaaccgctcc agatgaatgg ttttatctgg cattcccagt gcttttaagg
    37141 cggcttccgt ctcatccatc atcgccgccg gaccacaaat aaatgcctca tcataaagac
    37201 gaaaattaat gagcgaggcc ccaagtgact gtaatttttc accgtcaata cgcccgtgaa
    37261 gcagatcgct gtcgagggtt tcctgactga aaatgcacaa caactgtaaa cgctgaggat
    37321 atttgtcttt caggtctgcc agtgcctggc gaaacatcat gctctggctg gtacggttac
    37381 cgtagatcag ggtgaactga ctttcaggct cggtttgtaa agtggtggcg ataatcgcca
    37441 gcattggcgt aatacctgat cctgctgcaa tagccagata gcggccctgg cgttcggcct
    37501 gtggctgata gccgaaatgc ccctgcggca ccatgacctc cagcgtcata ccctggcgga
    37561 tgtgttcgcg ggcatagcgg gagaaacgtc cgccttcaat ggctttcacc gccacactaa
    37621 tttcgccagg cagatagctg cggcaaatgg agtaacaacg gcgtaattct tcaccatcaa
    37681 agctggcttt taaggtcaaa tgttgaccgg ggcgaaagcg atacgcctcc tgcaaaggct
    37741 ggggcaccgc aaaggtaatg gtcaccgcat cacgggtttc cggctccact tttgccaccg
    37801 ttaaggaatg aaacgttgtc atggcatcct caaatacatt tgaaataatc gaaaggttcg
    37861 cggcaactat cgcagcgata caatgctttg caggccgtgg aaccaaattc actgataagt
    37921 gtggtatgga cgctggcgca gcgcgggcaa cgtacttctg gcggcaaatg ggcatggcaa
    37981 ctgtgtccgg cgggcgggct aatgccatac tgtcgcagac gctcacgggc atcgggggtc
    38041 atccagtcgg tggtccatgc cgggtcgagt tgcagcacaa cctgaacggg ggtaaacccg
    38101 tgggttgtca ttgcctcacg tatcgcgcca atcaaatgtt ccgttgccgg acaaccggaa
    38161 taagtcggcg taaagccgat cacccatcct tctcccatct gtgtcacatt acgcaccatg
    38221 cctaaatcgg taatggtcag caccgggatc tccgggtccg ggatctggct gagtagcgcc
    38281 catatctcat gaacctgtgg cggtgcaatg gtggccagac gttgcatacc catctcctct
    38341 gttaccattg ctgaccgggc aagacacgct ggagatactg catttctgcc agcatgggtc
    38401 caaggtgttc ggtatgcagt ccttttttac cgccagtgcg atacgcctgc tcttgcggta
    38461 cgttcaatgt ggcttcgttg atcccggcaa aaacttcggc ttcccacgct gcgcgtaaag
    38521 tgcgtggatc aaccgcaata ccctcttcac tcagtgcaat atcaatctcg tcggcatcga
    38581 acagttcggc agtaaaacgc cacaacttgt cgatggcctg ctgcatcttt tgccctgata
    38641 cgtcagtacc attgcccagt cgctccagcc agccacgact aaaacgcagg tgataacgcg
    38701 cttctttaat tgctttggca gaaatcgccg ccagttgcgg atcacggctt tccatcagac
    38761 gggtaaagag cgccacatgc catgcatcga tgaaatactg gcgtgcaatg gtgtcggcaa
    38821 aattgccgtt tggttgttca accaacaata agttgctgaa ctggcgctcg tctcgggtga
    38881 aggccagggt atcttcatca ccttctcccg ctaattcagc ggcatacgat aagaagttac
    38941 gtgcctgacc taataaatcg aggccaatgt ttgccagtgc gagatcgatt tccagttccg
    39001 gtgcgtgacc gcaccattcc cccaaccgct gggagagcac cagacagtta tcgcccaggc
    39061 gcaaggtgta agccgttaac tgattcattt ccgacctcac atgtgctcaa tgccatcagg
    39121 gatggtgtaa aacgttggat ggcgatagac cttgctttca gccggatcaa aaaattcacc
    39181 gcgttcttcc ggttgcgagg caacaatttc actcgccttc accacccaaa ttgaacatcc
    39241 ttcgctacga cgggtgtaag catcacgggc attttccagt gccatccgct catcggcagc
    39301 atgtaaactg ccaacatggc ggtgtgataa gccctgtttg ccacgcacga aaacttcgta
    39361 taacggccag taaacattac tcattttgaa tctccttatg cgaccttacg ggcaagttgt
    39421 ttttgtgcat gggccagcgc tgcttcccgt acccaggtac cttcttccca ggctttacgt
    39481 ttggcgtcga gcctctcctg attacaaatt ccgcgaccgt taattacttc gttaaactcc
    39541 tgccagtcga tctcaccaaa gcggtagtga ccgctttcag tgtcaaaatg cagatccggg
    39601 tcaggaacgg tcataccgag catttcaacc tgtggaacgg tgttgtccac gaaacgctgg
    39661 cggagttcgt cgttggtgaa acgtttgatt ttccaggcga gacttctggc gctgtttggc
    39721 gagttatcat cgtttggccc gaacatcatt aaggctggcc accagaaacg gttaatggcg
    39781 tcttgcaaca tctgcttttg agcttcgctg ccctgcgcca gtgccatgca ggcttcaaac
    39841 ccctgacgct ggtgaaaact ctcttctttg cagattttca ccattgctct ggcatacggg
    39901 ccataagaag ttcggcataa cgccacctga ttgacgatcg ctgcgccatc caccagccag
    39961 ccgataacgc cgatatcagc ccaactcaat gtcggataat taaagatgga ggaatatttc
    40021 atccgtccgt cgagcatttt ttggtagatg tcttctctgg cacagcccag tgtttcggcg
    40081 gcgctataga gatacagacc atgaccggct tcatcctgca ctttggctaa cagaatggct
    40141 ttacgccgca aggttggtgc gcgagtgatc cagttacctt caggcaacat accaacaatc
    40201 tcggagtgcg catgctgccc aatctggcgg atcagtgtct ttcggtaagc atcgggcatc
    40261 cagtcctgcg gctcgatagc cgtttcctgg gctatccgtt gctcaaagcg ttcttcttgg
    40321 gtcacgtcat cacctttacg attccagttt tcgtgaatta attaaaaaca cgaatcacgt
    40381 tgttttatga aagttacaca atggttaact aaaatcacaa attttgtatg tttgaactgt
    40441 gacggatttc gcaccctatt tttataaatc attatttatc agtgcattaa tgttttccgc
    40501 agttgctata cagatcgcat agttaacatt tcgttaaaag accccttgct ttttatgatt
    40561 cgcgatttaa ctattagcaa cagaaatgtg aaacatctgg agagtagcga tgcagcagtt
    40621 agccagtttc ttatccggta cctggcagtc tggccggggc cgtagccgtt tgattcacca
    40681 cgctattagc ggcgaggcgt tatgggaagt gaccagtgaa ggtcttgata tggcggctgc
    40741 ccgccagttt gccattgaaa aaggtgcccc cgccctccgc gcgatgacct ttatcgaacg
    40801 tgcggcgatg cttaaagcgg tcgctaaaca tctgctgagt gaaaaagagc gcttctatgc
    40861 tctttctgcg caaacaggcg caacgcgggc agacagttgg gttgatattg aaggtggcat
    40921 tgggacgtta tttacttacg ccagcctcgg tagccgggag ctgcctgacg atacgctgtg
    40981 gccggaagat gaattgatcc ccttatcgaa agaaggtgga tttgccgcgc gccatgtact
    41041 gacctcaaag tcaggcgtgg cagtgcatat taacgccttt aacttcccct gctggggaat
    41101 gctggaaaag ctggcaccaa cgtggctggg cggaatgcca gccatcatca aaccagctac
    41161 cgcgacggcc caactgactc aggcgatggt gaaatcaatt gtcgatagtg gtcttgttcc
    41221 cgaaggcgca attagtctga tctgcggtag tgctggcgac ttgttggatc atctggacag
    41281 ccaggatgtg gtgactttca cggggtcagc ggcgaccgga cagatgctgc gagttcagcc
    41341 aaatatcgtc gccaaatcta tccccttcac tatggaagct gattccctga actgctgcgt
    41401 actgggcgaa gatgtcaccc cggatcaacc ggagtttgcg ctgtttattc gtgaagttgt
    41461 gcgtgagatg accacaaaag ccgggcaaaa atgtacggca atccggcgga ttattgtgcc
    41521 gcaggcattg gttaatgctg tcagtgatgc tctggttgcg cgattacaga aagtcgtggt
    41581 cggtgatcct gctcaggaag gcgtgaaaat gggcgcactg gtaaatgctg agcagcgtgc
    41641 cgatgtgcag gaaaaagtga acatattgct ggctgcagga tgcgagattc gcctcggtgg
    41701 tcaggcggat ttatctgctg cgggtgcctt cttcccgcca accttattgt actgtccgca
    41761 gccggatgaa acaccggcgg tacatgcaac agaagccttt ggccctgtcg caacgctaat
    41821 gccagcacaa aaccagcaac atgctctgca actggcttgt gcaggcggcg gtagccttgc
    41881 gggaacgctg gtgacggctg atccgcaaat tgcgcgtcag tttattgccg acgcggcacg
    41941 tacgcatggg cgaattcaga tcctcaatga agagtcggca aaagaatcca ccgggcatgg
    42001 ctccccactg ccacaactgg tacatggtgg gcctggtcgc gcaggaggcg gtgaagaatt
    42061 aggtggttta cgagcggtga aacattacat gcagcgaacc gctgttcagg gtagtccgac
    42121 gatgcttgcc gctatcagta aacagtgggt gcgcggtgcg aaagtcgaag aagatcgtat
    42181 tcatccgttc cgcaaatatt ttgaggagct acaaccaggc gacagcctgt tgactccccg
    42241 ccgcacaatg acagaggccg atattgttaa ctttgcttgc ctcagcggcg atcatttcta
    42301 tgcacatatg gataagattg ctgctgccga atctattttc ggtgagcggg tggtgcatgg
    42361 gtattttgtg ctttctgcgg ctgcgggtct gtttgtcgat gccggtgttg gtccggtcat
    42421 tgctaactac gggctggaaa gcttgcgttt tatcgaaccc gtaaagccag gcgataccat
    42481 ccaggtgcgt ctcacctgta agcgcaagac gctgaaaaaa cagcgtagcg cagaagaaaa
    42541 accaacaggt gtggtggaat gggcggtaga ggtattcaat cagcatcaaa ccccggtggc
    42601 gctgtattca attctgacgc tggtggccag gcagcacggt gattttgtcg attaatcggt
    42661 gaatgaaggg caacggcgaa tagttgccct tttatttcac taaattttgt gacgttgtca
    42721 cattatgcat gatgtgtaca tctattttca gggcatccac tgtatgaaaa gctggcacac
    42781 ctgccaaaca acctggcagg tgcaggcaat cccctttaca tcagtactga taatgtgaac
    42841 ctgaccaaac cgcccacaga gcgcggttgc taacaagaac acaacatctg acgaggttaa
    42901 taatgggaag cccctctctg tattctgccc gtaaaacagc cctggcgttg gcagtcgcct
    42961 taagtttcgc ctggcaagcg ccggtatttg cccacggtgg tgaagcgcat atggtaccaa
    43021 tggatgaaac gcttaaagaa tttggtgccg atgtgcagtg ggacgactac gcccagatct
    43081 ttaccctgat taaagatggc gcgtacgtga aagtgaagcc tggtgcgcaa acagcaattg
    43141 ttaatggtca acctctggca ctgcaagtgc cggtagtgat gaaagacaat aaggcctggg
    43201 tttctgacac ctttattaac gatgttttcc agtccggtct ggatcaaacc tttcaggtag
    43261 aaaagcgccc tcacccactt aatgcgctaa ctgcggacga aattaaacag gccgttgaaa
    43321 ttgttaaagc ttccgcggac ttcaaaccca atacccgttt tactgagatc tccctgctac
    43381 cgccagataa agaagctgtc tgggcgtttg cgctggaaaa caaaccggtt gaccagccgc
    43441 gcaaagccga cgtcattatg ctcgacggca aacatatcat cgaagcggtg gtggatctgc
    43501 aaaacaacaa actgctctcc tggcaaccca ttaaagacgc ccacggtatg gtgttgctgg
    43561 atgatttcgc cagtgtgcag aacattatta acaacagtga agaatttgcc gctgccgtga
    43621 agaaacgcgg tattactgat gcgaaaaaag tgattaccac gccgctgacc gtaggttatt
    43681 tcgatggtaa agatggcctg aaacaagatg cccggttgct caaagtcatc agctatcttg
    43741 atgtcggtga tggcaactac tgggcacatc ccatcgaaaa cctggtggcg gtcgttgatt
    43801 tagaacagaa aaaaatcgtt aagattgaag aaggtccggt agttccggtg ccaatgaccg
    43861 cacgcccatt tgatggccgt gaccgcgttg ctccggcagt taagcctatg caaatcattg
    43921 agcctgaagg taaaaattac accattactg gcgatatgat tcactggcgg aactgggatt
    43981 ttcacctcag catgaactct cgcgtcgggc cgatgatctc caccgtgact tataacgaca
    44041 atggcaccaa acgcaaagtc atgtacgaag gttctctcgg cggcatgatt gtgccttacg
    44101 gtgatcctga tattggctgg tactttaaag cgtatctgga ctctggtgac tacggtatgg
    44161 gcacgctaac ctcaccaatt gctcgtggta aagatgcccc gtctaacgca gtgctcctta
    44221 atgaaaccat cgccgactac actggcgtgc cgatggagat ccctcgcgct atcgcggtat
    44281 ttgaacgtta tgccgggcct gagtataagc atcaggaaat gggccagccc aacgtcagta
    44341 ccgaacgccg ggagttagtg gtgcgctgga tcagtacagt gggtaactat gactacattt
    44401 ttgactggat cttccatgaa aacggcacta ttggcattga tgccggtgct acgggcatcg
    44461 aagcggtgaa aggtgttaaa gcgaaaacca tgcacgatga gacggcgaaa gatgacacgc
    44521 gctacggcac gcttatcgat cacaatatcg tgggtactac acaccaacat atttataatt
    44581 tccgcctcga tctggatgta gatggcgaga ataacagcct ggtggcgatg gacccagtgg
    44641 taaaaccgaa tactgccggt ggcccacgca ccagtaccat gcaagttaat cagtacaaca
    44701 tcggcaatga acaggatgcc gcacagaaat ttgatccggg cacgattcgt ctgttgagta
    44761 acccgaacaa agagaaccgc atgggcaatc cggtttccta tcaaattatt ccttatgcag
    44821 gtggtactca cccggtagca aaaggtgccc agttcgcgcc ggacgagtgg atctatcatc
    44881 gtttaagctt tatggacaag cagctctggg taacgcgtta tcatcctggc gagcgtttcc
    44941 cggaaggcaa atatccgaac cgttctactc atgacaccgg tcttggacaa tacagtaaag
    45001 ataacgagtc gctggacaac accgacgccg ttgtctggat gaccaccggc accacacatg
    45061 tggcccgcgc cgaagagtgg ccgattatgc cgaccgaatg ggtacatact ctgctgaaac
    45121 catggaactt ctttgacgaa acgccaacgc taggggcgct gaagaaagat aagtgattgt
    45181 ttcagacaaa aaaacggcac caggtgccgt tttttactta tgagcgaacc agattaatac
    45241 cgtacacaca ccgacttagt ttcacgccaa ctgtccagcc agtcggggcc aaaatcacgg
    45301 cccgttcctg actgcttcat cccaccaaac ggtaagttag cgtcaattaa ggtatggctg
    45361 tttacccaca ccgtccctgc ctgtaagcga tcgctatatt ccagagcctg ggagagattt
    45421 tgcgtccaga cactggcagt taagccatat tccgtgtcgt ttgccagttg taacgcctct
    45481 tctccatccg ctacccgcac caggtttacc accggaccaa acacctcttc acgagttaaa
    45541 cgcaatttag catcgggatt taccaccagc gttggcgcaa cataataccc ctctccggct
    45601 ggtccattca acccgcgaat cagctctgct tgctgtgcct gcgcatcgtc gaggaatgaa
    45661 cacactttgt cgcagtgcgc acgagaaacc aaagggttaa tctgtgcaac aggtgacatc
    45721 cccggtccca cttgcaacga ttttaccgcc tgctcaaatc cactaaccag cgtgtcaaac
    45781 aacggcgctt caatataaat tcgcgaactg gcggcgcata cttgcccctg attcaggaag
    45841 cttccggtca tcaagccttc aataacccat tgcggatcag catcttttaa tacaattgcc
    45901 gggtttttac cgcccagttc cagcgttaca cgcgttaagc gatcagcagc agttctggca
    45961 ataccttttc ccgtcgcggt tgaaccggta aaactgattt tcgcaacatg aggatgtgac
    46021 gtcagggccg cgccgcatac agcacctgac ccggtgacca cattaaaaac gccatcagga
    46081 ataccagctt cgctggccag ctccgccacg cgcaacatcg tcagtggcgt ggtttccgaa
    46141 ggcttaatca cgattgaaca gcctgctgcc agtgctggca tcaccttcca cataccaatc
    46201 atcaacggaa agttccatgg cacaattccc gctaccacgc caaccggctc tttacgcgtc
    46261 caggcctgat aacgcgcccc ctggggtaaa ggaatcgaca agtccagcgt cttacccgcg
    46321 attttggtcg ttaacccggc ggtataacgc atccagttca gcgtacagcc cacttcaaaa
    46381 gcacgagaaa tggcaattga cttgccttgc tccagggttt ccagttgcgc cagctcctca
    46441 ctgtgctgct ccaccagatc agcaaaacgt agcagaatac gttcacgctc tgcgggtaat
    46501 cgcccggccc agcgacgcga gacaaaggcc cgccaggcag acatgactgc gttatctaca
    46561 tccgcttcgt tggcatcagc agttgatgca atttcttgcc cggtggccgg atcaaagatc
    46621 gccaaccgtt tttcactttg tgcggggcca ggacgaccat caatataaag accgtgttga
    46681 cgatcgagaa actgttggac ctggcttaat actgctacat gcggctctgt catcactttt
    46741 ccttattatt tacccagtgt gatttcagtg tacgcagcga caagaaaagt cacttttttc
    46801 gctgtgacat tcgctttgtc agctgtgaca agctccgcaa atcgtgacaa taaccgctta
    46861 tgtctctatg cctgacaaga cttatttcct atagtcaggc tcattagctt cgtttattgc
    46921 aacacaaatg caacaataaa aatacatttc acagagcgaa aacgtgccat gaaccccgca
    46981 gtggataatg agtttcagca atggctttcc caaatcaatc aggtatgcgg aaattttacc
    47041 ggacgcctgc tgactgagcg ttacacgggt gtactggaaa cgcattttgc caaaggacta
    47101 aagctgagca ccgtgacaac caacggagtg aatttatacc gcacctggca ggaggtaaaa
    47161 ggcagcgacg atgcctggtt ttacaccgtt tttcagctta gtggtcaggc aataatggag
    47221 caggatgagc gtcaggtgca gattggcgct ggcgatatta cgttactcga tgcctcacgc
    47281 ccctgttcgc tttactggca ggagtcttct aaacagattt cattactttt gccacgcact
    47341 ctgctggaac aatattttcc ccatcaaaaa cctgtctgcg cagaaagact ggacgctgac
    47401 ttacccatgg tgcaactcag tcatcgcctg ttacaggaga gcatgaataa tccggcactt
    47461 tctgaaacag aaagtgaagc tgcgctacag gcgatggtgt gtctgctgcg cccggtactt
    47521 catcagcggg aatctgttca acctcgtcgt gaacgtcagt ttcaaaaagt ggttacgttg
    47581 atagacgata atattcgcga agagatatta cgcccggagt ggatagccgg agagacaggt
    47641 atgtcagtac gtagtttgta ccgaatgttt gccgataaag gtttggtagt cgcgcaatat
    47701 attcgtaacc gtcgtctcga tttttgtgca gatgcgattc gccatgccgc agatgatgaa
    47761 aaactggcag gcatcggctt tcattgggga ttttctgacc agagtcattt ttcaacggta
    47821 tttaagcaac gctttgggat gacgccaggc gagtatcgac gtaaattccg ctaactaatt
    47881 ggcgttgtgt gtactgcaac gccaattagt taaaacagat atttgactat gcatttttca
    47941 gggcgaaagg caatttgaca ggagtttttg aatattcacc ttgtttcagg aaaatagatc
    48001 aatcgctttc tcctgtagcg ttatcccgat attgcggttt tgctattaaa attttcgcac
    48061 ccatttaccg tttatccctt gtacatattc ccccggtttg gcccgagcta cgagcttttg
    48121 cccggcgagt ttcgcgatat catctacaga cacattattt tgctttgcca gttgttggta
    48181 actcgcttta cgttcggcat taatgtcggt tactaatttc tctgtttcag catccgtttt
    48241 cagcgcaacc agataaccat aaaatgtttc acctacccgc ccctgagttc tggcttcatc
    48301 cagagtcaat gccattacat tgctgcttac cagcccaaca agaaacgcac aaagaagtaa
    48361 tgttttcttc atcatcgcct cagaaaagat cgctacgagt ttcaagcagc tcttcgacat
    48421 ctttgtctgc cttgatgatg atctcatgct caattttaac gttcatattg atagtgatcg
    48481 gttccttggg cgcagcgact tcaatacgcg gcgtacaggc agccagcata aaagatgacg
    48541 tcaacgcagc cagtaaaatt ttcattgttt ttcctcacac tcttttcctt gcggacagtc
    48601 atttcccggc agacgtgcgt tctgctccaa ccatgcctgg agattatcgc caaagcgtaa
    48661 actgcgccac agcgtaaaaa tattctcttc atgatagtaa ttaaggttta ccgtaccgct
    48721 tttaccatca acgcgactgg tacctgtaat gttggcctgc atggttaata aaccgagatt
    48781 atctaagtta atttttgtcg atgaccggct aatttccata tagcgcaacc agttaattgc
    48841 tgaacccgca gtcatattgt ctttcaccac cgcatctgcg gtgtctttat ccagccgcaa
    48901 ggtcattggc ccgctatttg ccagccaacc atctttcact atccattttt cgttgtttag
    48961 ccataacggc aatgcaccac taaatgcccc ggacatggcg aattgtttcg gattgacggc
    49021 gctaaccagt tcgctggatg agagattatt cagccgcaac agcgccgggt catgttgcgg
    49081 catacgtaat tgtttcatca gtacgttacc gcctaacaca tcgacgctaa catcagtcaa
    49141 cagcaagggt tcttcttcag tccacggata acgcccttgc aaatcagccg taatattttt
    49201 cgctgtcacc agattaatca cttcggcaat tcgcaacgta acggggccgc gagtccccag
    49261 atgccaggct ccatcggcaa aacggaaagg caggacaaaa tcgacaccgt taacctgatt
    49321 atctggcatc caggcactac cgcctttcaa cacgccgtgt cctcccgcgc ggaatccttg
    49381 ttcaggcgca gcagaaaatg caacctgagc atacagttca ccatcgcgta agttcatctt
    49441 ccagtcgggt ggcaccagcg gctggaatac agtcagtgat tgttttggcc accaggcgtt
    49501 accgcgcaga cgaataccgt cccagcgacc atttacccga accgggccaa tctcaccagc
    49561 atgtaaatcg cctttaaaga ggaaatacgt cggatcgcgc ccatcgacgc taaattttaa
    49621 ggttgatggc ggtaacacac tgccgccagt gaacagcgtt tgcccggcgt ccagtgacag
    49681 cgcgccgctg aaggagggat gctgtgcgtc acgtacccag acgacgggct tgtcgagaat
    49741 taatcgcggc ttttctaccg tcatcgtacc gtattgtaac tggtcgaaac cggtggagag
    49801 atcggtcagc gtaatcgtgc tgtcatgcca ctcacctttt cctgcgacat cccaggtggc
    49861 attcatcggt gtaaaactcc cttttcccca gtagcgccac tgccagcggc cagcgtcagg
    49921 gagaaaatca ttcgccagac catccatatg cagcacgaaa tcgcccagtt cattttcatg
    49981 cgcctgcaaa atggcctgca gacgtccgtc aacaccacgt tgggtgactt ttacacccgc
    50041 taaaggccag cggatttcat cgatgtccag cgaatcgatg actcttccct ttgaacgaag
    50101 taacgcgcct ggctcaaagg ccagcgttgg gtcagtcaga cttccactta gctgcgcagg
    50161 taaacgggca tataaaatga ggtccgcctg tttcgcttca ccggtcagct gcagaggcag
    50221 ctgactgtta tccatgctta attttcctgg gccaaaatta agcaccgcgt tgcccttacc
    50281 cgcttgcccc tgggtcagca cactcagtcg tccgctgacc agagcgttct caagccctgc
    50341 ctgccagttg tcgactttga cacccagtcg gccactcaaa ggaaaacctg catacggcca
    50401 gctccagcga ccatcgctta cggtcaattg ttgacgagta atttgccacg gcaaatcgag
    50461 caacggatcg ccgttatccc gtgccagcac aatcaattgc ccgctatttt cctgccagtc
    50521 cagctctgca tccaccagtg acggttcctg cggcaagttt aacgtcgcag tagcatgccc
    50581 acttacagga agtccatccg gcacgagcgg catagtaaat tcccccacca gtttaaccgg
    50641 cggctgattt tcaaacgcga cgacatccag ttcgctgact gtaagttgtt gccctttcag
    50701 ctggccttga aatttaactt tttcgccctg ataacgcagt tgctggatat cagaggttaa
    50761 tgcgagagag agttttccct gccattcctg ccagggagaa aaaatcagtt tatcgatatt
    50821 gatccaggtg ttaggcagca tggactgcca ctgcgcgagg gtttttggag cagctggcga
    50881 ttgctccgtc tgcggcaatt tcgccagaca agcagaatca agttctaccg tgccgacgtt
    50941 caataaccag cggctgggat gtgaaaggct ggcgttggtg atatgtgcaa gctgacaatc
    51001 tcccaccaga taacggagat cgggaatgat taaaccttta cgcgtaatgc gtgggctttc
    51061 atctaatgca atacgtgtcc cgagcggtag ccagatgccc gccagcgtgg gaacccacag
    51121 cccgagcgtc atcagcagcg tcaacggcac aagaataatc agtaataaca gcgcgagaac
    51181 ggctttatat ttacccagca tgggtagtta atatcctgat ttagcgaaaa attaagcatt
    51241 caatacgggt attgtggcat gtttaaccgt tcagttgaag gttgcgccta cactaagcat
    51301 agttgttgat gaatttttca atatcgccat agctttcaat tatatttgaa attttgtaaa
    51361 atatttttag tagcttaaat gtgattcaac atcactggag aaagtcttat gaaactcgcc
    51421 gtttatagca caaaacagta cgacaagaag tacctgcaac aggtgaacga gtcctttggc
    51481 tttgagctgg aattttttga ctttctgctg acggaaaaaa ccgctaaaac tgccaatggc
    51541 tgcgaagcgg tatgtatttt cgtaaacgat gacggcagcc gcccggtgct ggaagagctg
    51601 aaaaagcacg gcgttaaata tatcgccctg cgctgtgccg gtttcaataa cgtcgacctt
    51661 gacgcggcaa aagaactggg gctgaaagta gtccgtgttc cagcctatga tccagaagcc
    51721 gttgctgaac acgccatcgg tatgatgatg acgctgaacc gccgtattca ccgcgcgtat
    51781 cagcgtaccc gtgacgctaa cttctctctg gaaggtctga ccggctttac tatgtatggc
    51841 aaaacggcag gcgttatcgg taccggtaaa atcggtgtgg cgatgctgcg cattctgaaa
    51901 ggttttggta tgcgtctgct ggcgttcgat ccgtatccaa gtgcagcggc gctggaactc
    51961 ggtgtggagt atgtcgatct gccaaccctg ttctctgaat cagacgttat ctctctgcac
    52021 tgcccgctga caccggaaaa ctaccatctg ttgaacgaag ccgccttcga tcagatgaaa
    52081 aatggcgtga tgatcgtcaa taccagtcgc ggtgcattga ttgattctca ggcagcaatt
    52141 gaagcgctga aaaatcagaa aattggttcg ttgggtatgg acgtgtatga gaacgaacgc
    52201 gatctgttct ttgaagataa atccaacgac gtgatccagg atgacgtatt ccgtcgcctg
    52261 tctgcctgcc acaacgtgct gtttaccggg caccaggcat tcctgacagc agaagctctg
    52321 accagtattt ctcagactac gctgcaaaac ttaagcaatc tggaaaaagg tgaaacctgc
    52381 ccgaacgaac tggtttaatc ttgccgctcc cctgcaatcc aggggagctg attcagataa
    52441 tccccaatga cctttcattc tctattctta aaatagtcct gagtcagaaa ctgtaattga
    52501 aaaccacaat gaagaaagta gccgcgtttg ttgcgctaag cctgctgatg gcgggatgtg
    52561 taagtaatga caaaattgct gttacgccag aacagctaca gcatcatcgc tttgtgctgg
    52621 aaagcgtaaa cggtaagccc gtgaccagcg ataaaaatcc gccagaaatc agctttggtg
    52681 aaaaaatgat gatttccggc agcatgtgta accgctttag cggtgaaggc aaactgtcta
    52741 atggtgaact gacagccaaa gggctggcaa tgacccgtat gatgtgcgct aacccgcagc
    52801 ttaatgaact cgataacacc attagcgaaa tgctgaaaga aggtgcacaa gtggatctga
    52861 ccgcgaacca gttaacgctg gcgaccgcga aacagacatt aacttataag ctggcggatt
    52921 taatgaatta atagctgcca cagctcccgg cggcaagtga ctgttcgcta cagcgtttgc
    52981 cgttgggtaa tgcacacatc ccaatcgccg taccatccag ttgacgggca acagaaagcg
    53041 aaccgccgat cattgcacaa tttgcttctc cactactgga catcgacgct tttaaacctg
    53101 gcgctacgtg cgccgcagtc gcctgctgaa caggttcact actacacgcc gacaacaata
    53161 aagcggcaca ccctacccaa aacgctgctc gcatctcttc ttcctctgat cttcaagcca
    53221 aacgacaccg ccataaataa taggcagcac agagggcggc gtcgagagct gtcctgcgcg
    53281 ttgccccgca tttttacttt tttatggcta tttttttgcc ctctgtttga tcaaaacatt
    53341 cattacgctg atgtggggga cacaaaagcg aaaatgcaga agaaagccat ttgctaaaat
    53401 tgaaagatta ctactgggcg cgcagcaatt tcgtgcgccc ctcatttgcg caatgtaagg
    53461 gtgtcatatg attactattg acggtaatgg cgcggttgct tcggtcgcat ttcgcaccag
    53521 tgaagttatc gccatctacc ctattacccc cagttccacg atggcagaac aggctgatgc
    53581 ctgggccgga aacggcttaa agaacgtttg gggagacaca ccacgcgtgg ttgaaatgca
    53641 gtcggaagcg ggtgctatcg ctaccgtgca tggcgctttg cagacgggtg ccctttcaac
    53701 atcgtttacg tcatcgcagg gtttgctgct gatgatcccg acgctgtaca aactggcagg
    53761 cgaactaaca ccgtttgtcc tgcatgtagc ggcacgtacc gttgccacac atgcactctc
    53821 tatttttggc gatcattccg acgttatggc ggtgcgccag acgggttgcg cgatgttgtg
    53881 tgcagcaaac gtccaggaag cgcaagactt tgctctcatt tcgcaaatcg cgacgctgaa
    53941 aagccgcgtg ccatttattc atttctttga tggtttccgc acgtcccacg aaatcaataa
    54001 aattgtcccg ctggccgatg acacgattct tgatctcatg ccgcaggtcg aaattgatgc
    54061 tcatcgcgcc cgggcactca acccggaaca tccggtgatc cgcggtacgt ccgccaatcc
    54121 tgacacttat ttccagtctc gcgaagccac caacccatgg tacgacgcgg tctatgacca
    54181 tgttgaacag gcgatgaatg atttctctgc cgcgacaggt cgtcagtatc agccgtttga
    54241 atattacggg catccgcaag cggaacgggt gattatcctg atgggctctg ccattggcac
    54301 ctgtgaagaa gtggttgatg aattgctaac ccgtggcgaa aaagttggcg tgctgaaagt
    54361 tcgcctgtac cgtcccttct ccgccaaaca cttgctgcaa gctctgccgg gatccgtacg
    54421 cagcgtggcg gtactggaca gaaccaaaga acccggtgcc caggcagaac cgctctatct
    54481 ggatgtaatg accgcactgg cagaagcctt taataatggc gagcgcgaaa ctctgccccg
    54541 tgtcattggt gggcgctatg gtctttcatc caaagaattt ggcccggact gtgtactggc
    54601 ggtatttgcc gagctcaacg cggctaaacc gaaagcgcgc tttacggttg gtatttacga
    54661 tgatgtgacc aatctgtcac tgccgttgcc ggaaaacacc ctgccaaact cggcgaaact
    54721 ggaagccttg ttttatggcc ttggtagtga tggcagcgtt tccgcgacca aaaacaatat
    54781 caagattatc ggtaattcca cgccgtggta cgcacagggc tattttgttt acgactccaa
    54841 aaaggcgggc ggcctgacgg tttctcacct tcgcgtgagc gaacagccga ttcgttccgc
    54901 ttatctcatt tcccaggctg attttgttgg ctgccaccag ttgcagttta tcgataaata
    54961 tcagatggct gagcgtttaa aacctggcgg cattttcctg ctcaacacgc cgtacagcgc
    55021 agatgaagtg tggtcgcgct tgccgcagga agttcaggcc gtgctaaatc agaaaaaagc
    55081 gcgcttctat gtgattaacg cggcgaaaat cgcccgcgaa tgtggcctgg cggcccgtat
    55141 taataccgtc atgcagatgg cttttttcca tctgacgcaa attctgcctg gcgatagcgc
    55201 cctcgcagaa ttgcagggtg cgattgccaa aagttacagt agcaaaggcc aggatctggt
    55261 ggaacgcaac tggcaggctc tggcgctggc gcgtgaatcc gtagaagaag ttccgttgca
    55321 accggtaaat ccgcacagcg ccaatcgacc gccagtggtt tccgatgccg cccctgattt
    55381 cgtgaaaacc gtaaccgcag cgatgctcgc agggcttggt gatgccctcc ccgtttcggc
    55441 gctgccgcca gacggcacct ggccgatggg caccactcgc tgggaaaaac gcaatatcgc
    55501 cgaagagatc cccatctgga aagaggaact ctgtacccaa tgtaaccact gcgttgccgc
    55561 ttgcccacac tcagctattc gcgcaaaagt ggtgccgcct gaagcgatgg aaaacgcccc
    55621 tgccagcctg cattcgctgg atgtgaaatc gcgtgatatg cgcgggcaga aatatgtctt
    55681 gcaggtggca ccggaagatt gcaccggttg taacctgtgc gtcgaagttt gcccggcgaa
    55741 agaccgtcag aatccagaga ttaaagccat caatatgatg tctcgcctgg aacatgtcga
    55801 agaagagaaa atcaattacg atttcttcct caacctgcca gaaatcgacc gtagcaaact
    55861 ggaacgtatt gatattcgta catcgcagct gattacaccg ctgtttgaat attcaggtgc
    55921 ttgctccggt tgtggcgaga cgccgtatat taaattactg actcagctct atggcgaccg
    55981 gatgttgatc gctaacgcca ctggctgttc ttcaatttat ggcggtaacc tgccctctac
    56041 accgtatacc accgatgcca acggtcgtgg gccggcatgg gcgaactctc tatttgaaga
    56101 taatgccgaa tttggccttg gtttccgcct gacggtcgat caacaccgtg tccgcgtgct
    56161 gcgtctgctg gatcaatttg ccgataaaat cccggcggaa ttactgacgg cgttgaaatc
    56221 agacgccacg ccagaggttc gtcgtgaaca ggttgcagct ttacgccagc aactcaacga
    56281 tgttgccgaa gcacatgaac tgctacgtga tgcagatgca ctggtggaaa aatcaatctg
    56341 gctgattggt ggtgatggct gggcttacga tatcggcttt ggcggtctgg atcatgtatt
    56401 gagtttgacg gaaaacgtca acattctggt gctggatacg caatgctatt ccaacaccgg
    56461 tggtcaggca tcgaaagcga caccgctggg tgcagtaact aaatttggcg agcacggcaa
    56521 acgtaaagcg cgtaaagatc ttggcgtcag tatgatgatg tacggtcatg tttatgtggc
    56581 gcagatttct ctcggcgcgc agctgaacca gacggtgaaa gcgattcagg aagcggaagc
    56641 gtatccgggg ccatcgctga tcattgctta tagcccgtgt gaagagcatg gttacgatct
    56701 ggcactcagc cacgaccaga tgcgccaact cacagctacc ggcttctggc cgctatatcg
    56761 ctttgatccg cgtcgtgccg atgaaggcaa actgccgctg gccttggatt cacgcccgcc
    56821 gtcagaagca ctggaagaaa cgttacttca cgagcaacgt ttccgtcggc tgaattcgca
    56881 gcagccagaa gtggcagaac agttatggaa agatgctgca gctgatttgc aaaaacgcta
    56941 tgacttcctg gcacaaatgg ccggaaaagc ggaaaaaagc aacaccgatt aaatgctctg
    57001 gataaggatt atccaattct aaaaaaaaag cccggacgac tgttcgggct tgtcttttta
    57061 tatgttggaa aatcagtggc aatgcaatgg cccaacagaa aataaaatct gtgacaataa
    57121 aggcat
//
LOCUS       DAPHDW010000013        54114 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.013, whole genome shotgun sequence.
ACCESSION   DAPHDW010000013 DAPHDW010000000
VERSION     DAPHDW010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 54114)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 54114)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54114
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.013"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            257..1351
                     /gene="gcvT"
                     /locus_tag="RH057_000759"
     CDS             257..1351
                     /gene="gcvT"
                     /locus_tag="RH057_000759"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HDV3127175.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQAGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPADRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            1375..1764
                     /gene="gcvH"
                     /locus_tag="RH057_000760"
     CDS             1375..1764
                     /gene="gcvH"
                     /locus_tag="RH057_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HDV3127176.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYETLLEDE"
     gene            1883..4756
                     /gene="gcvP"
                     /locus_tag="RH057_000761"
     CDS             1883..4756
                     /gene="gcvP"
                     /locus_tag="RH057_000761"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HDV3127177.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASDVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWPEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQNELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            5022..5765
                     /locus_tag="RH057_000762"
     CDS             5022..5765
                     /locus_tag="RH057_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3127178.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LITQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDTLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(5822..7255)
                     /gene="bglA"
                     /locus_tag="RH057_000763"
     CDS             complement(5822..7255)
                     /gene="bglA"
                     /locus_tag="RH057_000763"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HDV3127179.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTGGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDDMFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKVVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHENPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYCCNPDDVMFAQESMRERYVFTDVQLRGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GAGDMSRSRKKSFNWYQEVIASNGEKL"
     gene            7300..7611
                     /locus_tag="RH057_000764"
     CDS             7300..7611
                     /locus_tag="RH057_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV3127180.1"
                     /translation="MQPNDITFFQRFQDDILAGRKTITIRDESESHFKTGDVLRVGRF
                     EDDGYFCTIEVTATSTVTLDTLTEKHAEQENMTLTELIKVIADIYPGQTQFYVIEFKC
                     L"
     gene            7775..8434
                     /locus_tag="RH057_000765"
     CDS             7775..8434
                     /locus_tag="RH057_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HDV3127181.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(8511..9491)
                     /gene="ygfZ"
                     /locus_tag="RH057_000766"
     CDS             complement(8511..9491)
                     /gene="ygfZ"
                     /locus_tag="RH057_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HDV3127182.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            9734..10000
                     /gene="sdhE"
                     /locus_tag="RH057_000767"
     CDS             9734..10000
                     /gene="sdhE"
                     /locus_tag="RH057_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HDV3127183.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRNRERGPVAI"
     gene            9981..10388
                     /locus_tag="RH057_000768"
     CDS             9981..10388
                     /locus_tag="RH057_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HDV3127184.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQEWCIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(10428..10949)
                     /gene="fldB"
                     /locus_tag="RH057_000769"
     CDS             complement(10428..10949)
                     /gene="fldB"
                     /locus_tag="RH057_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HDV3127185.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIVALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            11061..11957
                     /gene="xerD"
                     /locus_tag="RH057_000770"
     CDS             11061..11957
                     /gene="xerD"
                     /locus_tag="RH057_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HDV3127186.1"
                     /translation="MKQDLARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            11982..12692
                     /gene="dsbC"
                     /locus_tag="RH057_000771"
     CDS             11982..12692
                     /gene="dsbC"
                     /locus_tag="RH057_000771"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HDV3127187.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKEMKEFLDEHQKMTSGK"
     gene            12698..14431
                     /gene="recJ"
                     /locus_tag="RH057_000772"
     CDS             12698..14431
                     /gene="recJ"
                     /locus_tag="RH057_000772"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HDV3127188.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGETLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLDPSLLQGEVVSDGPLSPAEMTMEVAQLLCDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            14522..15620
                     /gene="prfB"
                     /locus_tag="RH057_000773"
     CDS             join(14522..14596,14598..15620)
                     /gene="prfB"
                     /locus_tag="RH057_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HDV3127189.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            15630..17147
                     /gene="lysS"
                     /locus_tag="RH057_000774"
     CDS             15630..17147
                     /gene="lysS"
                     /locus_tag="RH057_000774"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV3127190.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(17190..17738)
                     /gene="idi"
                     /locus_tag="RH057_000775"
     CDS             complement(17190..17738)
                     /gene="idi"
                     /locus_tag="RH057_000775"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HDV3127191.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPSGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLHGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            17793..17864
                     /locus_tag="RH057_000776"
     CDS             17793..17864
                     /locus_tag="RH057_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127192.1"
                     /translation="MINQVSVYRQPPVLSGCRQVKTI"
     gene            complement(17861..17986)
                     /locus_tag="RH057_000777"
     CDS             complement(17861..17986)
                     /locus_tag="RH057_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127193.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(17988..19436)
                     /gene="uacT"
                     /locus_tag="RH057_000778"
     CDS             complement(17988..19436)
                     /gene="uacT"
                     /locus_tag="RH057_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HDV3127194.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKEAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIIRGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVTRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLKVRTVRMWLLMRKLKKNEHGE"
     gene            19872..21791
                     /gene="ygfT"
                     /locus_tag="RH057_000779"
     CDS             19872..21791
                     /gene="ygfT"
                     /locus_tag="RH057_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     YgfT"
                     /protein_id="HDV3127195.1"
                     /translation="MNKFIAAEAAECIGCHACEIACAVAHNQENWPLSHSDFRPRIHV
                     VGKGQAANPVACHHCNNAPCVTACPVNALTFQSDSVQLDEQKCIGCKRCAIACPFGVV
                     EMVDTIAQKCDLCNQRSSGTQACIEVCPTQALRLMDDKGLQWIKVARQRKTAAGKASS
                     DAQPSRSAALLPVNSRKGADKISASERKTHFGEIYCGLDPLQATYESDRCVYCAEKAN
                     CNWHCPLHNAIPDYIRLVQEGKIIEAAELCHQTSSLPEICGRVCPQDRLCEGACTLKD
                     HSGAVSIGNLERYITDTALAMGWRPDVSKVVPRSEKVAVIGAGPAGLGCADILARAGV
                     QVDVFDRHPEIGGMLTFGIPPFKLDKTVLSQRREIFTAMGIDFHLNCEIGRDITFSDL
                     TSEYDAVFIGVGTYGMMRADLPHEDAPGVIQALPFLTAHTRQLMGLPESEEYPLTDVE
                     GKRVVVLGGGDTTMDCLRTSIRLNAASVTCAYRRDEVSMPGSRKEVVNAREEGVEFQF
                     NVQPQYIACDEDGRLTAVGLIRTAMGEPGPDGRRRPRPVAGSEFELPADVLIMAFGFQ
                     AHAMPWLQGSGIKLDKWGLIQTGDVGYLPTQTHLKKVFAGGDAVHGADLVVTAMAAGR
                     QAARDMLTLFDTKAS"
     gene            21791..22279
                     /locus_tag="RH057_000780"
     CDS             21791..22279
                     /locus_tag="RH057_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417362.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV3127196.1"
                     /translation="MKSLIIVNPADCIGCRTCEVACVVAHPSEQELNADVFLPRLKVQ
                     RLDSISAPVMCHQCENAPCVGACPVGALTMGEQVVQTNSARCIGCQSCVSACPFGMIT
                     IQSLPGDTRQQIVKCDLCEQREEGPACVESCPTQALQLLTERELRRVRQQRIVASGEN
                     PL"
     gene            complement(22315..23682)
                     /gene="ghxQ"
                     /locus_tag="RH057_000781"
                     /gene_synonym="ygfQ"
     CDS             complement(22315..23682)
                     /gene="ghxQ"
                     /locus_tag="RH057_000781"
                     /gene_synonym="ygfQ"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxQ"
                     /protein_id="HDV3127197.1"
                     /translation="MSGDILQTPDAPKPQGALDNYFKITARGSTVRQEVLAGLTTFLA
                     MVYSVIVVPGMLGKAGFPPAAVFVATCLVAGFGSLLMGLWANLPMAIGCAISLTAFTA
                     FSLVLGQQISVPVALGAVFLMGVIFTAISVTGVRTWILRNLPMGIAHGTGIGIGLFLL
                     LIAANGVGMVIKNPIEGLPVALGAFTSFPVMMSLLGLAVIFGLEKCRVPGGILLVIIA
                     ISIIGLIFDPAVKYHGLVAMPSLTGEDGKSLIFSLDIMGALQPTVLPSVLALVMTAVF
                     DATGTIRAVAGQANLLDKDNQIINGGKALTSDSVSSIFSGLVGAAPAAVYIESAAGTA
                     AGGKTGLTATVVGALFLLILFLSPLSFLIPGYATAPALMYVGLLMLSNVSKLDFNDFI
                     DAMAGLVCAVFIVLTCNIVTGIMLGFVTLVVGRVFAREWQKLNIGTVIITAALVAFYA
                     GGWAI"
     gene            complement(23718..25034)
                     /gene="guaD"
                     /locus_tag="RH057_000782"
     CDS             complement(23718..25034)
                     /gene="guaD"
                     /locus_tag="RH057_000782"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HDV3127198.1"
                     /translation="MSGEHTLKAVRGSFIDVTRTVDNPEEIASALRFIEDGLLLIKQG
                     KVEWFGEWEDGKHQIPDTIRVRDYRGKLIVPGFVDTHIHYPQSEMVGAYGEQLLEWLN
                     KHTFPTERRYEDLEYAREMSAFFIKQLLRNGTTTALVFGTVHPQSVDALFEAASHINM
                     RMIAGKVMMDRNAPDYLLDTAESSYHQSKELIERWHKNGRLLYAITPRFAPTSSPEQM
                     AMAQRLKEEYPDTWVHTHLCENKDEIAWVKSLYPDHDGYLDVYHQYGLTGKNCVFAHC
                     VHLEEKEWDRLSETKSSIAFCPTSNLYLGSGLFNLKKAWQKKVKVGMGTDIGAGTTFN
                     MLQTLNEAYKVLQLQGYRLSAYEAFYLATLGGAKSLGLDDLIGNFLPGKEADFVVMEP
                     TATPLQQLRYDNSVSLVDKLFVMMTLGDDRSIYRTYVDGRLVYERN"
     gene            complement(25052..26452)
                     /gene="xanQ"
                     /locus_tag="RH057_000783"
     CDS             complement(25052..26452)
                     /gene="xanQ"
                     /locus_tag="RH057_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanQ"
                     /protein_id="HDV3127199.1"
                     /translation="MSDINNAGSDLIFELEDRPPFHQALVGAITHLLAIFVPMVTPAL
                     IVGAALQLSAETTAYLVSMAMIASGIGTWLQVNRYGIVGSGLLSIQSVNFSFVTVMIA
                     LGSSMKSDGFHEELIMSSLLGVSFVGAFLVVGSSFILPYLRRVITPTVSGIVVLMIGL
                     SLIKVGIIDFGGGFAAKSSGTFGNYEHLGVGLLVLIVVIGFNCCRSPLLRMGGIAIGL
                     CVGYIASLCLGMVDFSSMRNLPLITIPHPFKYGFSFSFHQFLVVGTIYLLSVLEAVGD
                     ITATAMVSRRPIQGEEYQSRLKGGVLADGLVSVIASAVGSLPLTTFAQNNGVIQMTGV
                     ASRYVGRTIAVMLVILGLFPMIGGFFTTIPSAVLGGAMTLMFSMIAIAGIRIIITNGL
                     KRRETLIVATSLGLGLGVSYDPEIFKILPASIYVLVENPICAGGLTAILLNIILPGGY
                     RQENVLPGITSAEEMD"
     gene            complement(26617..29487)
                     /locus_tag="RH057_000784"
     CDS             complement(26617..29487)
                     /locus_tag="RH057_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     Mo/Fe-S-binding subunit"
                     /protein_id="HDV3127200.1"
                     /translation="MIIHFTLNGAPQELTVNPGENVQKLLFNMGMHSVRNSDDGFGFA
                     GSDAIIFNGNIVNASLLIAAQLEKADIRTAESLGKWNELSLVQQAMVDVGVVQSGYND
                     PAAALIITDLLDRIAAPTREEIDDALSGLFSRDAGWQQYYQVIELAVARKNNPQATID
                     IAPTFRDDLEVIGKHYPKTDAAKMVQAKPCYVEDRVTADACVIKMLRSPHAHALITHL
                     DVSKAEALPGVVHVITHLNCPDIYYTPGGQSAPEPSPLDRRMFGKKMRHVGDRVAAVV
                     AESEEIALEALKLIDVEYEVLKPVMSIDEAMAEDAPVVHDEPVVYVAGVPDTLEDDNS
                     HAAQRGEHMIINFPIGSRPRKNIAASIHGHIGDMDKGFADADVIIERTYNSTQAQQCP
                     TETHICFTRMDGDRLVIHASTQVPWHLRRQVARLVGMKQHKVHVIKERVGGGFGSKQD
                     ILLEEVCAWATCVTGRPVLFRYTREEEFIANTSRHVAKVTVKLGAKKDGRLTAVKMDF
                     RANTGPYGNHSLTVPCNGPALSLPLYPCDNVDFQVTTYYSNICPNGAYQGYGAPKGNF
                     AITMALAELAEQLQIDQLEIIERNRVHEGQELKILGAIGEGKAPTSVPSAASCALEEI
                     LRQGREMIQWSSPKPQNGDWHIGRGVAIIMQKSGIPDIDQANCMIKLESDGTFIVHSG
                     GADIGTGLDTVVTKLAAEVLHCPPQDVHVISGDTDHALFDKGAYASSGTCFSGNAARL
                     AAENLREKILFHGAQMLGEAVADVQLATPGVVRGKKGEVSFGDIAHKGETGTGFGSLV
                     GTGSYITPDFAFPYGANFAEVAVNTRTGEIRLDKFYALLDCGTPVNPELALGQIYGAT
                     LRAIGHSMSEEIIYDAEGHPLTRDLRSYGAPKIGDIPRDFRAVLVPSDDKVGPFGAKS
                     ISEIGVNGAAPAIATAIHDACGIWLREWHFTPEKILTALEKI"
     gene            complement(29484..30263)
                     /locus_tag="RH057_000785"
     CDS             complement(29484..30263)
                     /locus_tag="RH057_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase
                     FAD-binding subunit"
                     /protein_id="HDV3127201.1"
                     /translation="MIEQFFRPDSVEQALELKRRYQDEAVWFAGGSKLNATPTRTDKK
                     IAISLQDLELDWVDWDNGALRIGAMSRLQPLRDARFIPAALREALGFVYSRHVRNQST
                     IGGEIAARQEESVLLPVLLALDAELVFGNGETLSIEDYLACPCDRLLTEIIIKDPYRT
                     CATRKISRSQAGLTVVTAAVAMTDHDGMRIALDGVASKALRLHDVEKQNLEGNALEQA
                     VANAIFPQEDLRGSVAYKRYISGVLVADLYADCQQAEEEAV"
     gene            complement(30314..31642)
                     /gene="ssnA"
                     /locus_tag="RH057_000786"
     CDS             complement(30314..31642)
                     /gene="ssnA"
                     /locus_tag="RH057_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HDV3127202.1"
                     /translation="MLILKNVTAVQLHPAKVQEGVDIAIENDVIVAIGDALTQRYPDA
                     SYKEMHGRIVMPGIVCSHNHFYSGLSRGIMANIAPCPDFISTLKNLWWRLDRALDEES
                     LYYSGLICSLEAIKSGCTSVIDHHASPAYIGGSLSTLRDAFLKVGLRAMTCFETTDRN
                     NGIKELQEGVEENIRFARQIDEAKKAATEPYLVEAHIGAHAPFTVPDAGLEMLREAVK
                     ATGRGLHIHAAEDLYDVSYSHHWYGKDLLARLAQFDLIDSKTLVAHGLYLSKDDITLL
                     NQRDAFLVHNARSNMNNHVGYNHHLSDIRNLALGTDGIGSDMFEEMKFAFFKHRDAGG
                     PLWPDSFAKALTNGNELMSRNFGAKFGLLEAGYKADLTICDYNSPTPLLADNIAGHIA
                     FGMGSGSVHSVMVNGVMVYEDRQFNFDCDSIYAQARKAAASMWRRMDALA"
     gene            complement(31645..34743)
                     /gene="ygfK"
                     /locus_tag="RH057_000787"
     CDS             complement(31645..34743)
                     /gene="ygfK"
                     /locus_tag="RH057_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenate reductase subunit YgfK"
                     /protein_id="HDV3127203.1"
                     /translation="MGDIMRPIPFEELLTRIFDEYQQQRSIFGIPEQQFYSPVKGKTV
                     SVFGETCATPVGPAAGPHTQLAQNIVTSWLTGGRFIELKTVQILDRLELEKPCIDAED
                     ECFNTEWSTEFTLLKAWDEYLKAWFALHLLEAMFQPSDSGKSFIFNMSVGYNLEGIKQ
                     PPMQQFIDNMMDASDHPKFAQYRDTLNKLLQDDAFLARHGLQEKRESLQALPARIPTS
                     MVHGVTLSTMHGCPPHEIEAICRYMLEEKGLNTFVKLNPTLLGYARVREILDVCGFGY
                     IGLKEESFDHDLKLTQALEMLERLMALAKEKSLGFGVKLTNTLGTINNKGALPGEEMY
                     MSGRALFPLSINVAAVLSRAFDGKLPISYSGGASQLTIRDIFDTGIRPITMATDLLKP
                     GGYLRLSACMRELEGSDAWGLDHVDVERLNRLAADALTMEYTQKHWKPEERIEVAEDL
                     PLTDCYVAPCVTACAIKQDIPEYIRLLGEHRYADALELIYQRNALPAITGHICDHQCQ
                     YNCTRLDYDSALNIRELKKVALEKGWDEYKQRWHKPAGSGSRHPVAVIGAGPAGLAAG
                     YFLARAGHPVTLFEREANAGGVVKNIIPQFRIPAELIQHDIDFVAAHGVKFEYGCSPD
                     LTVEQLKNQGFHYVLIATGTDKNSGVKLAGDNQNVWKSLPFLREYNKGTALKLGKHVV
                     VVGAGNTAMDCARAALRVPGVEKATIVYRRSLQEMPAWREEYEEALHDGVEFRFLNNP
                     ECFDADGTLTLRVMSLGEPDEKGRRRPVETNETVTLHVDSLITAIGEQQDTEALNAMG
                     VPLDKNGWPDVDHNGETRLTDVFMIGDVQRGPSSIVAAVGTARRATDAILSRENIRSH
                     QNNKYWNNVNPAEIYQRKGDISITLVNSDDRDAFVAQEAARCLECNYVCSKCVDVCPN
                     RANVSIAVPGFQNRFQTLHLDAYCNECGNCAQFCPWNGKPYKDKITVFSLSQDFDNSS
                     NPGFLVEDCRVRVRLNNQSLVLNIDSEGQFDNVPPELNDMCRIISHVHQHHHYLLGRV
                     EV"
     gene            complement(35065..35643)
                     /gene="mocA"
                     /locus_tag="RH057_000788"
     CDS             complement(35065..35643)
                     /gene="mocA"
                     /locus_tag="RH057_000788"
                     /EC_number="2.7.7.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor cytidylyltransferase"
                     /protein_id="HDV3127204.1"
                     /translation="MSAIDCIITAAGLSSRMGQWKMMLPWEQGTILDTSIKNALQFCS
                     RIILVTGYRGNELHERYANQSNITIIHNPDYAQGLLTSVKAAVPAVQTEHCFLTHGDM
                     PTLTIDIFRKIWSLRNDGAILPLHNGIPGHPILVSKPCLIQAIQRPNVTNMRQALLMG
                     DHYSVEIENAEIILDIDTPDDFITAKERYTEI"
     gene            35746..36516
                     /gene="yqeC"
                     /locus_tag="RH057_000789"
     CDS             35746..36516
                     /gene="yqeC"
                     /locus_tag="RH057_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium-dependent hydroxylase
                     accessory protein YqeC"
                     /protein_id="HDV3127205.1"
                     /translation="MKSIVDPSALVIDLGAQKRPTVISVVGAGGKTSLLFWLAELLQA
                     SGRRVLITTTTHMFMPTSHWPVVFCRDPAMLPHASLTSPISFCFHSWKANQGKVQGFT
                     PEAIDALVQRPECDVILIEADGSRGMPLKAPDEHEPCIPKSSCCVIAVMGGHTLGAKV
                     STENVHRWSQFADITGLTPDATLQLSDLVALVRHPQGAFKNVPQGCRRVWFINRFSQC
                     ENAIAQSELLQPLQQHDVEAIWLGDIQEHPAIARRFVN"
     gene            36564..38189
                     /locus_tag="RH057_000790"
     CDS             36564..38189
                     /locus_tag="RH057_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF2563 family selenium-dependent molybdenum
                     hydroxylase system protein"
                     /protein_id="HDV3127206.1"
                     /translation="MNIFTEAAKLEEQNCPFAMAQIVDSRGSTPRHSAQMLVRADGSI
                     VGTIGGGMVERKVIEESLQALQERKPRLFHGRMARNGADAVGSDCGGAMSVFISVHGM
                     RPRLVLIGAGHVNRAIAQSAALLGFDIAVADIYRESLNPELFPPSTTLLHAESFGAAV
                     EALDIRPDNFVLIATNNQDREALDKLIEQPIAWLGLLASRRKVQLFLRQLREKGVAEE
                     HIARLHAPVGYNIGAETPQEIAISVLAEILQVKNNAPGGLMMKPSHPSGHQLVVIRGA
                     GDIASGVALRLYHAGFKVIMLEVEKPTVIRCTVAFAQAVFDGEMTVEGVTARLATSSA
                     EAMKLTERGFIPVMADPACSLLDELKPLCVVDAILAKQNLGTRADMAPVTIALGPGFT
                     AGKDCHAVIETNRGHWLGQVIYSGCAQENTGVPGNIMGHTTRRVIRAPAAGIMRSNVK
                     LGDLVKEGDVIAWIGEHEIKAPLTGMVRGLLNDGLAVVGGFKIGDIDPRGETADFTSV
                     SDKARAIGGGVLEALMMLMHQGVKATKEVLEVA"
     gene            complement(38230..39162)
                     /gene="arcC"
                     /locus_tag="RH057_000791"
     CDS             complement(38230..39162)
                     /gene="arcC"
                     /locus_tag="RH057_000791"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV3127207.1"
                     /translation="MSKKIVLALGGNALGDDLAGQMKAVKITSQAIVDLIAQGHEVIV
                     THGNGPQVGMINQAFEAAAKTEAHSPMLPMSVCVALSQGYIGYDLQNALREELLSRGI
                     NKPVATLVTQVEVDANDPAFLNPTKPIGSFFTEQEAEQLTKQGYTLKEDAGRGYRRVV
                     ASPKPVDIIEKETVKALVDAGQVVITVGGGGIPVIREGNHLRGASAVIDKDWASARLA
                     EMIDADMLIILTAVEKVAINFGKENEQWLDRLSLSDAERLIEEGHFAKGSMLPKVEAA
                     ASFARSRAGREALITVLSKAKEGIEGKTGTVICQ"
     gene            complement(39210..40595)
                     /gene="hyuA"
                     /locus_tag="RH057_000792"
     CDS             complement(39210..40595)
                     /gene="hyuA"
                     /locus_tag="RH057_000792"
                     /EC_number="3.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-phenylhydantoinase"
                     /protein_id="HDV3127208.1"
                     /translation="MRVLIKNGTVVNADGQAKQDLLIESGIVRQLGNNISPQLPYEEI
                     DATGCYVFPGGVDVHTHFNIDVGIARSCDDFFTGTRAAACGGTTTIIDHMGFGPNGCR
                     LRHQLEVYRGYAAHKAVIDYSFHGVIQHINHAILDEIPMMVEEGLSSFKLYLTYQYKL
                     NDDEVLQALRRLHESGALTTVHPENDAAIASKRAEFIAAGLTAPRYHALSRPLECEAE
                     AIARMINLAQIAGNAPLYIVHLSNGLGLDYLRLARANHQPVWVETCPQYLLLDERSYD
                     TEDGMKFILSPPLRNVREQDKLWCGISDGAIDVVATDHCTFSMAQRLQISKGDFSRCP
                     NGLPGVENRMQLLFSSGVMTGRITPERFVELTSAMPARLFGLWPQKGLLAPGSDGDVV
                     IIDPRQSQQIQHRHLHDNADYSPWEGFTCQGAIVRTLSRGETIFCDGTFTGKAGRGRF
                     LRRKPFVPPVL"
     gene            complement(40648..41859)
                     /locus_tag="RH057_000793"
     CDS             complement(40648..41859)
                     /locus_tag="RH057_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HDV3127209.1"
                     /translation="MAKNIPFKLILEKAKDYQADMTRFLRDMVAIPSESCDEKRVVHR
                     IKEEMEKVGFDKVEIDPMGNVLGYIGHGPRLVAMDAHIDTVGIGNIKNWDFDPYEGME
                     TDELIGGRGTSDQEGGMASMVYAGKIIKDLGLEDEYTLLVTGTVQEEDCDGLCWQYII
                     EQSGIRPEFVVSTEPTDCQIYRGQRGRMEIRIDVQGVSCHGSAPERGDNAIFKMGPIL
                     GELQELSQRLGYDEFLGKGTLTVSEIFFTSPSRCAVADSCAVSIDRRLTWGETWEGAL
                     DEIRALPAVQKANAVVSMYNYDRPSWTGLVYPTECYFPTWKVEEDHFTVKALVNAYEG
                     LFGKAPVVDKWTFSTNGVSIMGRHGIPVIGFGPGKEPEAHAPNEKTWKSHLVTCAAMY
                     AAIPLSWLATE"
     gene            complement(41917..43113)
                     /gene="dpaL"
                     /locus_tag="RH057_000794"
     CDS             complement(41917..43113)
                     /gene="dpaL"
                     /locus_tag="RH057_000794"
                     /EC_number="4.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopropionate ammonia-lyase"
                     /protein_id="HDV3127210.1"
                     /translation="MSVFSLKIDIADNKFFNGETSPLFSQSQAKLARQFHQKIAGYRP
                     TPLCALDDLANLFGVKKILVKDESKRFGLNAFKMLGGAYAIAQLLCEKYHLDIETLSF
                     EHLKNAIGEKMTFATTTDGNHGRGVAWAAQQLGQNAVIYMPKGSAQERVDAILNLGAE
                     CIVTDMNYDDTVRLTMQHAQQHGWEVVQDTAWEGYTKIPTWIMQGYATLADEAVEQMR
                     EMGVTPTHVLLQAGVGAMAGGVLGYLVDVYSPQNLHSIIVEPDKADCIYRSGVKGDIV
                     NVGGDMATIMAGLACGEPNPLGWEILRNCATQFISCQDSVAALGMRVLGNPYGNDPRI
                     ISGESGAVGLGVLAAVHYHPQRQSLMEKLALNKDAVVLVISTEGDTDVKHYREVVWEG
                     KHAVAP"
     gene            complement(43171..44358)
                     /gene="ygeW"
                     /locus_tag="RH057_000795"
     CDS             complement(43171..44358)
                     /gene="ygeW"
                     /locus_tag="RH057_000795"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="knotted carbamoyltransferase YgeW"
                     /protein_id="HDV3127211.1"
                     /translation="MKTVNELIKDINSLTSHLHEKDFLLTWEQTPDELKQVLDVAAAL
                     KALRAENISTKVFNSGLGISVFRDNSTRTRFSYASALNLLGLAQQDLDEGKSQIAHGE
                     TVRETANMISFCADAIGIRDDMYLGAGNAYMREVGAALDDGYKQGVLPQRPALVNLQC
                     DIDHPTQSMADLAWLREHFGSLENLKGKKIAMTWAYSPSYGKPLSVPQGIIGLMTRFG
                     MDVTLAHPEGYDLIPDVVEVAKNNAKASGGSFRQVTSMEEAFKDADIVYPKSWAPYKV
                     MEQRTELLRANDHEGLKALEKQCLAQNAQHKDWHCTEEMMELTRDGEALYMHCLPADI
                     SGVSCKEGEVTEGVFEKYRIATYKEASWKPYIIAAMILSRKYAKPGALLEQLLKEAQE
                     RVK"
     gene            44837..46615
                     /locus_tag="RH057_000796"
     CDS             44837..46615
                     /locus_tag="RH057_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent transcriptional regulator"
                     /protein_id="HDV3127212.1"
                     /translation="MELATTQSVLMQIQPTIQRFARMLASVLQLEVEIVDENLCRVAG
                     TGAYGKFLGRQLSGNSRLLRHVLETKTEKVVTQSRFDPLCEGCDSKENCREKAFLGTP
                     VILQDRCVGVISLIAVTHEQQEHISDNLREFSDYVRHISTIFVSKLLEDQGPGDNISK
                     IFATMIDNMDQGVLVVDDESRVQFVNQTALKTLGVVQNNIIGKPIRFRPLTFESNFTH
                     GHMQHIVSWDDKSELIIGQLHNIQGRQLFLMAFHQSHTSFSVANAPDEPHIEQLVGEC
                     RVMRQLKRLISRIAPSPSSVMVVGESGTGKEVVARAIHKLSGRRNKPFIAINCAAIPE
                     QLLESELFGYVKGAFTGASANGKTGLIQAANTGTLFLDEIGDMPLMLQAKLLRAIEAR
                     EILPIGASSPIQVDIRIISATNQNLAQFIAEGKFREDLFYRLNVIPITLPPLRERQED
                     IELLVHYFLHLHTRRLGSVYPGIAPDVVEILRKHRWPGNLRELSNLMEYLVNVVPSGE
                     VIDSTLLPPNLLNNGTTEQSDVTEVSEAHLSLDDAGGTALEEMEKQMIREALSRHNSK
                     KQVADELGIGIATLYRKIKKYELLNT"
     gene            complement(46655..47134)
                     /gene="xdhC"
                     /locus_tag="RH057_000797"
     CDS             complement(46655..47134)
                     /gene="xdhC"
                     /locus_tag="RH057_000797"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HDV3127213.1"
                     /translation="MNHSETITIECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGAVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(47131..48009)
                     /gene="xdhB"
                     /locus_tag="RH057_000798"
     CDS             complement(47131..48009)
                     /gene="xdhB"
                     /locus_tag="RH057_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HDV3127214.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATSIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(48020..50317)
                     /gene="xdhA"
                     /locus_tag="RH057_000799"
     CDS             complement(48020..50317)
                     /gene="xdhA"
                     /locus_tag="RH057_000799"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
                     /protein_id="HDV3127215.1"
                     /translation="MEAREATATGESCMRVDAIAKVTGRARYTDDYVMAGMCYAKYVR
                     SPIAHGYAVSINDEQARSLPGVLAIFTWEDVPDIPFATAGHAWTLDENKRDTADRALL
                     TRHVRHHGDAVAIVVARDELTAEKAAQLVSIEWQELPVITTPEAALAEDAAPIHNGGN
                     LLKQSTMSTGNVQQTIDAADYQVQGHYQTPVIQHCHMESVTSLAWMEDDSRITIVSST
                     QIPHIVRRVVGQALDIPWSCVRVIKPFVGGGFGNKQDVLEEPMAAFLTSKLGGIPVKV
                     SLSREECFLATRTRHAFTIDGQMGVNRDGTLKGYSLDVLSNTGAYASHGHSIASAGGN
                     KVAYLYPRCAYAYSSKTCYTNLPSAGAMRGYGAPQVVFAVESMLDDAATALGIDPVEI
                     RLRNAAREGDANPLTGKRIYSAGLPECLEKGRKIFEWEKRRAECQNQQGNLRRGVGVA
                     CFSYTSNTWPVGVEIAGARLLMNQDGTINVQSGATEIGQGADTVFSQMVAETVGVPVS
                     DVRVISTQDTDVTPFDPGAFASRQSYVAAPALRSAALLLKEKIIAHAAVMLHQSAMNL
                     TLIKGHIVLVERPEEPLMSLKDLAMDAFYHPERGGQLSAESSIKTTTNPPAFGCTFVD
                     LTVDIALCKVIINRILNVHDSGHILNPLLAEGQVHGGMGMGIGWALFEEMIIDAKSGV
                     VRNPNLLDYKMPTMPDLPQLESAFVEINEPQSAYGHKSLGEPPIIPVAAAIRNAVKMA
                     TGVAINTLPLTPKRLYEEFHLAGLI"
     gene            50732..51487
                     /locus_tag="RH057_000800"
     CDS             50732..51487
                     /locus_tag="RH057_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HDV3127216.1"
                     /translation="MSAGRLNKKSLGIVMLLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            51566..51639
                     /locus_tag="RH057_000801"
     tRNA            51566..51639
                     /locus_tag="RH057_000801"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:51598..51600,aa:Gly,seq:ccc)
     gene            51873..53483
                     /locus_tag="RH057_000802"
     CDS             51873..53483
                     /locus_tag="RH057_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001613747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127217.1"
                     /translation="MKGKSALTLLLAGIFSCGTCQATGAEVTSESVFNILNSTGAATD
                     KSYLSLNPDKYPNYRLLIHSAKLQNEIKSHYTKDEIQGLLTLTENTRKLTLTEKPWGT
                     FILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWDYMSTPD
                     QIKRMQDVISNPKRLDGVPGQMNVVHIRFDSNSPVMADVQEEGKPQLWNHKQNDALGL
                     YLDLLIQAIDTDTINAEDWQKGDRLKSVALLIAYLDKANFYVMKDSGAWEEDARLNTS
                     SVALVTSGLERLSNLLSKKDSVFVSDLLREAKANELDEPLSTTRLNHLIDKGYERITL
                     QLDLGGESPGYLEKDKHYREADAALLNVIYPANLAKINTRRKEQVLKIVKKLAGPYGI
                     KRYEKDNYQSANFWFNDIKTDTDQNSHAKREKSFIPSTEAEWFFDSWYAKSAAIVYKE
                     SRKEEYLNDSVQFMNRSLAQITGENMIGANGRSVPEMALPESYNYIHKSGTLHEAPSP
                     IIPLNWSKASMTLMLKEMSNLINDEGIK"
     gene            53496..53615
                     /locus_tag="RH057_000803"
                     /pseudo
     CDS             53496..53615
                     /locus_tag="RH057_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314987.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion protein"
     gene            53615..53980
                     /locus_tag="RH057_000804"
     CDS             53615..53980
                     /locus_tag="RH057_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127218.1"
                     /translation="MFGLRKVNRNTHTFETTFQNWKENGEDVALLMPEFSAKWLPIAE
                     ESGSWSGWVLLGEIFPLISSELAGMALMPETERLIGEWLSLSSSPLNLKHPELKYNRL
                     HVGKVFDGVLSPAQPLIRI"
BASE COUNT        13415 a        13352 c        14114 g        13233 t
ORIGIN      
        1 agctttctaa tggcatcatc tttatataga aactggtgtg ttttttggtt ttttattctg
       61 tcgcgatttt ggcgtttttt aaccataagc taatgtgatg atcaatttta ccttatggtt
      121 aacagtctgt ttcggtggta agttcaggca aaagagaacg attgcgttgg ggaccgggag
      181 tggctccgat gctgggtttc gtggtgataa tttcaccatg aaaagttgtc agccccgctt
      241 attcaatgag gacaagatgg cacaacagac tcctttgtac gaacaacaca cgctttgcgg
      301 cgctcgcatg gtggatttcc acggctggat gatgccgctg cattacggtt cgcaaatcga
      361 cgaacatcat gcggtacgta ccgatgccgg aatgtttgat gtgtcacata tgaccatcgt
      421 cgatctccgc ggcagccgca cccgggagtt tctgcgttat ctgctggcga acgatgtggc
      481 gaagctcacc aaaagcggca aagcccttta ctcggggatg ttgaatgcct ctggcggtgt
      541 gatagatgac ctcatcgtct actactttac tgaagatttc ttccgcctcg ttgttaactc
      601 cgccacccgc gaaaaagacc tctcctggat tacccaacac gctgaacctt tcggcatcga
      661 aattaccgtt cgtgatgacc tttccatgat tgccgtgcaa gggccgaatg cgcaggcaaa
      721 agctgccaca ctgtttaatg acgcccagcg tcaggcggtg gaagggatga aaccgttctt
      781 tggcgtgcag gcgggcgatc tgtttattgc caccactggt tataccggtg aagcgggcta
      841 tgaaattgcg ctgcccaatg aaaaagcggc cgatttctgg cgtgcgctgg tggaagcggg
      901 tgttaagcca tgtggcttgg gcgcgcgtga cacgctgcgt ctggaagcgg gcatgaatct
      961 ttatggtcag gagatggacg aaactatttc tcctttagcc gccaacatgg gctggactat
     1021 cgcctgggaa ccggcagatc gtgactttat cggtcgtgaa gccctggaag tgcagcgtga
     1081 gcatggtaca gaaaaactgg ttggtctggt gatgaccgaa aaaggcgtgc tgcgtaatga
     1141 actgccggta cgctttaccg atgcgcaggg caaccagcat gaaggcatta tcaccagcgg
     1201 tactttctcc ccgacgctgg gttacagcat tgcgctggcg cgcgtgccgg aaggtattgg
     1261 cgaaacggcg attgtgcaaa ttcgcaaccg tgaaatgccg gttaaagtga caaaacctgt
     1321 ttttgtgcgt aacggcaaag ccgtcgcgtg atttactttt ttggagattg attgatgagc
     1381 aacgtaccag cagaactgaa atacagcaaa gaacacgaat ggctgcgtaa agaagccgac
     1441 ggcacttaca ccgttggtat aaccgaacat gcccaggagc tgttaggcga tatggtgttt
     1501 gttgacctgc cggaagtggg cgcaacggtt agcgcgggcg atgactgcgc ggttgccgaa
     1561 tcagtaaaag cggcgtcaga catttatgcg ccagtaagcg gtgaaatcgt ggcggtaaac
     1621 gacgcactga gcgattcccc ggaactggtg aacagcgaac cgtatgcagg tggctggatc
     1681 tttaaaatca aagccagcga tgaaagcgaa ctggaatcac tgctggatgc gaccgcatac
     1741 gaaacattgt tagaagacga gtaacggctt tattcctctt ctgcgggaga ggatcagggt
     1801 gaggaaaatt tatgcctcac cctcactctc ttcgtaagga gagaggttca caattcactg
     1861 cacgtttcag gaaccatcgc tcatgacaca gacgttaagc cagcttgaaa acagcggcgc
     1921 ttttattgaa cgccatatcg gaccggacgc cgcgcaacag caagaaatgc tgaatgccgt
     1981 tggtgcacaa tcgttaaacg cgctgaccgg ccagattgtg ccgaaagata ttcagcttgc
     2041 gacaccaccg caggttggcg caccggcgac cgaatacgcc gcactggcag aactcaaggc
     2101 tattgccagt cgcaataaac gcttcacgtc ttacatcggc atgggttaca ccgccgtgca
     2161 gctaccgccg gttatcctgc gtaacatgct ggaaaatccg ggctggtata ccgcgtacac
     2221 tccgtatcaa cctgaagtct cccagggccg ccttgaagca ctgctcaact tccagcaggt
     2281 aacgctggat ttgactggac tggatatggc ctctgcttct cttctggacg aggccaccgc
     2341 tgccgccgaa gcaatggcga tggcgaaacg cgtcagcaaa ctgaaaaatg ccaaccgctt
     2401 cttcgtggct tccgatgtgc atccgcaaac gctggatgtg gtccgtactc gtgccgaaac
     2461 ctttggtttt gaagtgattg tcgatgacgc gcaaaaagtg ctcgaccatc aggacgtctt
     2521 cggcgtgctg ttacagcagg taggcactac cggtgaaatt cacgactaca ctgcgcttat
     2581 tagcgaactg aaatcacgca aaattgtggt cagcgttgcc gccgatatta tggcgctggt
     2641 gctgttaact gcgccgggta aacagggcgc ggatattgtt tttggttcgg cgcaacgctt
     2701 cggcgtgccg atgggctacg gtggcccaca cgcggcattc tttgcggcga aagatgaata
     2761 caaacgctca atgccgggcc gtattatcgg tgtatcgaaa gatgcagctg gcaataccgc
     2821 gctgcgcatg gcgatgcaga ctcgcgagca acatattcgt cgtgagaaag cgaactccaa
     2881 catttgtact tcccaggtac tgctggcaaa catcgccagc ctgtatgccg tttatcacgg
     2941 cccggttggc ctgaaacgta tcgctaaccg cattcaccgt ctgaccgata ttctggcggc
     3001 gggcctgcaa caaaaaggtc tgaaactgcg ccatgcgcac tatttcgaca ccttgtgtgt
     3061 ggaagtggcc gacaaagcgg gcgtactgac gcgtgccgaa gcggctgaaa tcaacctgcg
     3121 tagcgatatc ctgaacgccg tggggatcac ccttgatgaa accaccacgc gtgaaaacgt
     3181 aatgcagctt ttcagcgtgc tgctgggcga taaccacggg ctggagatcg acacgctgga
     3241 caaagacgtg gctcacgaca gccgctctat ccagcctgcg atgctgcgcg acgacgaaat
     3301 cctcacccat ccggtgttta atcgctacca cagcgaaacc gaaatgatgc gctatatgca
     3361 ctcgctggag cgtaaagatc tggcgctgaa tcaggcgatg atcccgctgg gttcctgcac
     3421 catgaaactg aacgccgccg ccgagatgat ccccatcacc tggccggaat ttgccgaact
     3481 gcacccgttc tgcccgccgg aacaggccga aggttatcag cagatgattg cgcagctggc
     3541 tgactggctg gtgaaactga ccggttacga cgccgtttgt atgcagccga actctggcgc
     3601 acagggcgaa tacgcgggcc tgctggcgat tcgtcattat catgaaagcc gcaacgaagg
     3661 gcatcgcgat atctgcctga tcccggcttc tgcgcacgga actaaccccg cttctgcaca
     3721 tatggcagga atgcaggtgg tggttgtggc gtgtgataaa aacggcaaca tcgatctgac
     3781 tgatctgcgc gcgaaagcgg aacaggcggg cgataacctc tcctgtatca tggtgactta
     3841 tccttctacc cacggcgtgt atgaagaaac gatccgtgaa gtgtgtgaag tcgtgcatca
     3901 gttcggcggt caggtttacc ttgatggcgc gaacatgaac gcccaggttg gcatcacctc
     3961 gccgggcttt attggcgcgg acgtttcgca cctcaacctg cataaaactt tctgcattcc
     4021 gcacggcggt ggtggtccgg gtatgggacc gatcggtgtg aaagcgcatc tggcaccgtt
     4081 tgtaccgggt catagcgtgg tgcaaatcga aggcatgtta acccgtcagg gcgcagtttc
     4141 tgcggcaccg ttcggtagcg cctctatcct gccaatcagc tggatgtaca tccgcatgat
     4201 gggcgcagaa gggctgaaaa aagcaagcca ggtggcaatc ctcaacgcca actatattgc
     4261 cagccgcctg caggatgcct tcccggtgct gtataccggt cgcgacggtc gcgtggcgca
     4321 cgaatgtatt ctcgatattc gcccgctgaa agaagaaacc ggcatcagcg agctggatat
     4381 tgccaagcgc ctgatcgact acggtttcca cgcgccgacc atgtcgttcc cggtggcggg
     4441 tacgctgatg gttgaaccga ctgaatctga aagcaaagtg gaactggatc gctttatcga
     4501 cgcgatgctg gctatccgcg cagaaatcga ccaggttaaa gccggtgtct ggccgctgga
     4561 agataacccg ctggtgaacg cgccgcacat tcagaacgaa ctggtcgccg agtgggcgca
     4621 tccgtacagc cgtgaagttg cagtattccc ggcaggtgtg gcagacaaat actggccgac
     4681 ggtgaaacgt ctggatgatg tttacggcga ccgtaacctg ttctgctcct gcgtaccgat
     4741 tagcgaatac cagtaattca ctgattcgac tatcttctaa aggcgcttcg gcgccttttt
     4801 agtcagatga caaagtacaa aagcgctcag acagtcccct cacccctttg gggagagggt
     4861 tagggtgagg ggaacaggcc ggcactggcg cgaatattta ccctcacccc ggccctctcc
     4921 ctgaaagggc gaggggaaaa gcgtgccaac attgaagatt gagccagttt gttagcaatc
     4981 tcaaagatac gtcaacgaat taatttttct cggaaaaaca aatggctata gcacttgtga
     5041 ccggtggcag tcgcggcatc gggcgggcaa ctgcattact gttggcgcaa gaagggtata
     5101 cggtggcggt taattatcag caaaacctcc acgcggcgca ggaagtgatg aacttaataa
     5161 cgcaagccgg tggcaaagca ttcgtgctcc aggcggatat cagcgacgaa aaccaggtcg
     5221 ttgcgatgtt tacagcaatc gatcagcacg atgaaccgct agcagcgctg gtcaataacg
     5281 ccgggatctt gtttacccag tgcaccgttg aaaaccttac cgcagagcga atcaaccgag
     5341 tactttccac caacgtgacg ggatattttc tctgctgccg cgaggcggta aaacgcatgg
     5401 cgcttaaaaa tggtggcagt ggcggcgcta tcgtcaatgt ctcttcggtg gcctcacggt
     5461 tgggttcgcc aggggaatat gttgattacg cggcatcgaa aggggcgatt gatacgttaa
     5521 ccaccggact atcgctggaa gtcgccgcgc aggggatccg cgttaactgc gtgcggccag
     5581 ggtttattta taccgaaatg cacgccagcg gcggcgagcc tggacgcgtc gatcgcgtta
     5641 agtcgaacat ccccatgcag cgtggtggac aggcagaaga ggtcgcgcag gccattgtct
     5701 ggctactaag tgataaagcc tcttacgtca cgggaagttt tatcgatttg gcgggcggga
     5761 aataaaacag ggaagttgtc tgaccggatg caacaagtat tgcatccggt acttcatcga
     5821 cttaaagctt ctcgccgttg ctggcaatca cctcttggta ccagttaaag ctcttcttac
     5881 gtgaacgcga catatcgcca gcaccgtcgt catgtttatt cacatagata aagccgtagc
     5941 gtttgctgta ctgcccggtg gtgaacgata cgcagtcgat gcagccccac ggtgtgtagc
     6001 ccatcagatc cacgccatcg taagtcaccg cttttttcat ctcttcgata tgggcgcgca
     6061 ggtagtcaat gcggtagtcg tcgttgatgc tgccatcttc ttccactttg tcgtaagcgc
     6121 caaaaccgtt ttcgacaata aacagcggcc tctgataacg ctcatacagt tcgcaaaggg
     6181 catagcgcag acctactgga tcaatctgcc agccccagtc agacgcttta acatacgggt
     6241 tcggtacgct gccttcgaaa ccagagatcg catcgccggt gccgccttcg gccttcactg
     6301 cgttggtcat gtaatagctg aaaccaagat aatcgcaggt gccttcacgc agtacatcga
     6361 gatcgccgtc ttccattttg atgttaaatc cgcgacgctc ccactcgttc aacacatagg
     6421 acgggtaata gccgcgtagc tgcacatcgg taaagacgta gcgttcgcgc atcgactcct
     6481 gagcgaacat cacatcgtcc gggttacagc agtagggata gagcggcacc atcgccagca
     6541 tacagccgac tttcatctcc gggttaatgc gacgcgcagc tttcaccgcc agggcgctgg
     6601 cgacaaactg gtgatgcagc acctgataca tcgtctcttc cgggttctca tgctcggtat
     6661 aaaccacgcc ggagcagcag taaccgaaca gcggtgcacg ccagttacgc tggttgttaa
     6721 tttcgttgaa ggtcatccag tatttgactt tatgcttata gcgttcaaac accacttccg
     6781 cgaaacggac aaagaaatca accactttac ggttggtcca gctaccgtat tgctgcacca
     6841 gatgcagcgg catttcaaag tgggagaggg tgatcaccgg ttcgatgttg tatttcagca
     6901 gttcatcgaa catatcatcg tagaacttca gcccttcttc gtttggctga gcttcatcac
     6961 cttttggaaa aatacgcgtc caggcgatgg aagtccgaaa acatttgaag cccatttcgg
     7021 caaatagctt gatgtcttcc ttgtagtgac cataaaaatc aacggcttca tggtttggat
     7081 agtatttgcc tggcaagact tctttggtga tttcgcgcgg cacgccgtgt gcgccaccgg
     7141 tcagaacgtc acaaatgctc ggccctttgc cgcctttgtt ccagccgcct tcgacctgat
     7201 gagcggcaac tgcgccgccc cataagaaat ctttcggtaa ggtgagtttt ttcactatca
     7261 taggctccag atgagtcaat tcttacagga aatagtacca tgcagccaaa cgacatcact
     7321 ttttttcaac gtttccagga tgacattctg gctgggcgta aaaccatcac catccgcgac
     7381 gagtctgaat cgcacttcaa aacgggtgat gtgcttcgtg tcgggcgttt tgaagatgac
     7441 ggttattttt gcacgattga agtcaccgca acctcaaccg taacgctgga tacgctgacg
     7501 gaaaaacatg cagagcagga aaatatgacc ctgactgaac tgataaaggt cattgccgac
     7561 atctatcccg gtcagacaca gttttatgtg attgaattta aatgtcttta aattgaactc
     7621 acgcctgtta gttgaaattt cgcatttaat ttcatgattt ttaggagatt agtctcaaat
     7681 cagaatttat tttagctaac aggtgttcac tggaactatt ctcagttacg ctggagaggt
     7741 atacatgtgc gtgtacgttt ctccggagta agttatggtt cagaagcccc tcattaagca
     7801 gggatattcg ctggcagagg aaattgccaa cagcgtcagt cacggcattg ggttggtgtt
     7861 tggtatcgtt gggctggtgt tgctactggt tcaggcggtg gatctcaatg ccagcgccac
     7921 agcgataacc agttacagcc tctatggcgg cagtatgatc ctgctgttcc tcgcttcgac
     7981 gctctatcac gccattcctc atcaacgggc aaaaatgtgg ctgaagaaat ttgaccattg
     8041 cgctatttac ctgttgattg ccggaaccta cacgccgttt ttgctggtgg ggctggattc
     8101 tccgttagcg cgcgggttga tgattgttat ctggagcctg gcattgctgg gtattctgtt
     8161 taaactgacc atcgcgcacc gattcaaaat tttatctctg gtgacctatc tggcgatggg
     8221 ctggctgtcg ctggtggtaa tttatgaaat ggcagttaag ctcgcggcgg gcagcgttac
     8281 cttactggcg gtaggcggcg tggtttattc gctcggggtg attttctacg tctgcaaacg
     8341 cattccatac aaccatgcca tctggcacgg cttcgtgctc ggcggtagtg tgtgccactt
     8401 tctggcgatc tatttgtata ttgggcaggc gtaattgctg tgcctttgtc tgcatgaaaa
     8461 gacacatcag tgtcgcttca ggcaatagat gcccgaagcg atatagaaat ttactcttcg
     8521 agcgaatacg gcagcggctc gatatgcagt gtatttgcat cgtcgcgcac gcggaagatg
     8581 ctgtccggtt ccatatcgtt attcatcacc acctgcacca cgacctgacc atcttccagt
     8641 ttgaccgcag ccagcaccgt accggtacga cgccagtttt cgcccatttt cagctctaag
     8701 tcttcacctg cttctggcag tcggctggcg ctacctgcca gtaaccagag cgcacgtttg
     8761 ttggcaccgc ggaatttggc ccgcgccacc atctcttgtc cggtataaca gcctttctta
     8821 aagctgatac cgcccagcgc ctggagattg gtcgcctgtg ggataaactg cccgctgttg
     8881 gcggcatcaa tcaccgggaa acccgcttca atgtttaatg ccagccactg ttggctattg
     8941 ttcagttccg cttcaccgcg cagtttatcg gtcagcatat tagcagtagc ttcatcggtt
     9001 acgatcagga aacgttctgc cgggtgttca aaccatagca aagtggtcgc gccttctttg
     9061 actacctgtt tttctttcga aggcagttcg ctaaagagat ttgccagcgc ggcgcgcgcc
     9121 tgaaaaccgg caacaccaag cagcacacgc tcgtcgtctg gcgcgatggt cactttagag
     9181 aataccgcat attttttcag ttcagtcagc tgcggttcac gcacgctgcg ccgttcaatc
     9241 catgcaaagc catcgccgtc gcggaacaga cgtaaattgc tccacatttt acctttggcg
     9301 tcgcaatggg cggcgagcag gtgctgatct tctgccatct ggctgacatc tgctgtcacc
     9361 tgaccctgca tatatttttc gctgtccgcg ccagtaatgg tggcaagcgc ccagtcatca
     9421 agcgtcatca gcgtcagcgg taaacgggca gaagccgtag gctgacgggg aggaaaaggt
     9481 gtaaaagcca taatagagtc ctgagttgct taacggcgta ttaatgacta atggtaaaag
     9541 agctatgagg caatgcaagt gctttcacta cgcgatctgt gccttctgcg aggcgacttc
     9601 caaaaaaatg aagccattga attgttgtga attaatttcg cgatcgcgat ggtgtttact
     9661 gatattcccg taaaagaagg cgaaaaacac gttacaatag ccgggtactg gattttcgtg
     9721 agacaggaag aagatggaca ttaacaacaa agcccgcatt cattgggcat gccgccgtgg
     9781 tatgcgcgaa ctcgatattt caatcatgcc gtttttcgaa catgagtacg acagcttaag
     9841 cgatgacgaa aaacgcatct ttattcgtct gctggaatgt gacgatccgg acctgtttaa
     9901 ctggctgatg aatcacggta aaccggccga tgcagaactg gaaatgatgg tccgactcat
     9961 ccagacacgg aaccgggaac gtggtcctgt ggcaatctga tttgcgcgtc tcctggcgcg
    10021 cacagtggct ttccttgctg attcatgggc tggttgccgc tgttatttta ctcatgccct
    10081 ggccactcag ttacaccccg ttatggatgg tgttactttc gctggtggtg tttgattgcg
    10141 ttcgcagcca gcggcgtatt aatgctcgcc agggggaaat tcgcttgttg atggacgggc
    10201 gtttgcgttg gcaagggcag gagtggtgca tcgtcaaagc accgtggatg attaagagcg
    10261 gcatgatgct gcgtttacgt tctgatggcg gtaaacggca acatttatgg ctggcagccg
    10321 acagcatgga cgaagccgaa tggcgggatt tacggcggat tttgttgcaa caagagacgc
    10381 aaagataaag atgacggcaa agcatatgcg ctaagccgtc agtgagatca ggcgtaatgc
    10441 tctgccattt cgttgaggat ttgctcgcac cagctctgaa tacgctcgtc gctaaggtca
    10501 tactggttag tttcatccag cgccagaccc acgaacagtt gcccgtcagc aatcaccggt
    10561 ttcgggctgg taaattcata tccttccgtt ggccagtagc cgacgaactt cacgcctttg
    10621 gtcgagagtt tgtcatgcag cataccgagc gcatcgagga accactcgcc gtatcccagt
    10681 tgatcgccaa gcccatacag cgcaacaatt ttaccttcaa ggttcaggtc gtcgagctga
    10741 tcccagacgg cttcccagtc ttcctggatt tcaccaaaat cccaggtcgg gatacccaga
    10801 atgagcacat cgtactgctc cattaatttc ggggagtcgt ccttgaggtt atgtaaggtc
    10861 accagttctg ggccgataat atcgcggatt ttttctgccg ccatttcggt gtaacaggtg
    10921 ctggaaccgt aaaaaagacc catattcata acgtaagctt ctcgattctg ctgtggcttt
    10981 tgcagctagt gtatcagaat cgctttagtc acggcacaat aaggcataat ggatcaaatg
    11041 agtgaccata aggggcgcaa gtgaaacagg atctggcacg catcgagcag tttcttgatg
    11101 ctctgtggct ggaaaaaaat ctggctgaaa atacgttgaa cgcttaccgt cgcgatctgt
    11161 caatgatggt ggagtggttg catcaccgcg ggttgacgct ggcgacggcg caaagtgacg
    11221 atttgcaggc attactggca gaacggctgg agggcgggta taaagccacc agctcagcgc
    11281 gtttgctgag tgcagtgcgc cgattgttcc agtatcttta tcgcgaaaag tttcgtgaag
    11341 acgatcccag tgcgcatctc gcttcaccga aattgcccca gcgtttgcca aaagatttaa
    11401 gcgaagcgca ggtcgaacgt ttattacagg caccattaat tgatcagcca ctggagctac
    11461 gcgataaagc catgcttgaa gtgttgtatg ctaccgggct gcgtgtctct gaactggtcg
    11521 gactgacaat gagtgatatc agcctgcgtc agggcgtagt gcgggtcatt ggtaaaggca
    11581 acaaagagcg cctggtaccg ttaggtgaag aggcggttta ctggctggaa acctatctgg
    11641 aacatgggcg tccgtggctg ctgaatggtg tgtcaattga cgtgttgttt cccagccagc
    11701 gtgcgcagca gatgacgcga cagaccttct ggcaccgcat taaacattat gctgtgctgg
    11761 cgggtatcga cagcgaaaag ctgtcaccgc atgtattgcg tcacgctttt gccacccatt
    11821 tattaaatca tggtgcggat ttacgcgtgg tgcagatgct actgggccac agcgacctct
    11881 ccaccacgca aatttatacg catgtcgcta ccgagcgtct gcggcaactt catcaacagc
    11941 atcacccgcg ggcgtgatgt ctgaaaagaa cgggaagatt tatgaagaaa ggttttatgt
    12001 tgtttacttt gttagcggcg ttttcaggtt ttgctcaggc tgatgacgcg gcaattcaac
    12061 aaacgttagc caaaatgggc atcaaaagca gcgatattca gcccgcgccc gtagctggca
    12121 tgaagacagt tctgactaac agcggcgtgt tgtacatcac cgatgatggt aaacatatca
    12181 ttcaggggcc aatgtatgac gttagcggca cggctccggt caatgtcacc aataagatgc
    12241 tgttaaagca gttgaatgcg cttgaaaaag agatgatcgt ttataaagcg ccgcaggaaa
    12301 aacacgtcat caccgtgttt actgatatta cctgtggtta ctgccacaaa ctgcatgagc
    12361 aaatggcaga ctataacgcg ctggggatca ccgtgcgtta tcttgctttc ccgcgccagg
    12421 ggctggacag cgatgcagag aaagaaatga aagctatctg gtgtgcgaaa gataaaaaca
    12481 aagcgtttga tgatgtgatg gcaggtaaaa gcgtcgcacc agccagctgc gacgtggata
    12541 ttgccgacca ttacgcactt ggcgtccagc ttggcgttag cggtactccg gcagttgtgc
    12601 tgagcaatgg cacacttgtt ccgggttacc agccgccgaa agagatgaaa gaattcctcg
    12661 acgaacacca aaaaatgacc agcggtaaat aattcgcgtg aaacaacaga tacaacttcg
    12721 tcgccgtgaa gtcgatgaaa cggcagactt gcccgctgaa ttgcctccct tgctgcgccg
    12781 tttatacgcc agccggggcg tgcgcagtgc gcaagaactg gaacgcagtg ttaaaggtat
    12841 gttgccctgg cagcaactga gcggcgtcga aaaggccgtt gagatccttt acaacgcttt
    12901 tcgcgaagga acgcggatta ttgtggtcgg cgattttgac gccgacggcg cgaccagcac
    12961 ggctctaagc gtgctggcga tgcgctcgct tggttgcagc aatatcgact acctggtacc
    13021 aaaccgtttc gaagacggtt acggcttaag cccggaagtg gtcgatcagg cccatgcccg
    13081 tggcgcgcag ttaattgtca cggtggataa cggtatttcc tcccatgcgg gggttgaaca
    13141 cgctcgctcg ttgggcattc cggttattgt taccgatcac catttgccag gcgaaacatt
    13201 acccgcggcg gaagcgatca ttaaccctaa cttgcgcgac tgtaatttcc cgtcgaaatc
    13261 actggcaggc gtgggtgtgg cgttttatct gatgctggcg ctgcgcacct ttttgcgcga
    13321 tcagggctgg tttgatgagc gtggcatcgc aattcctaac ctggcagaac tgctggatct
    13381 ggtagcgctg gggacagtag cggacgtcgt gccgctggac gctaataatc gcattctgac
    13441 ctggcagggg atgagtcgca tccgtgccgg aaagtgccgt ccggggatta aagcgctgct
    13501 ggaagtggca aatcgcgatg cgcaaaaact cgccgccagc gatttaggtt ttgcgctggg
    13561 gccacgtctc aatgctgccg ggcgactgga cgatatgtcc gtcggtgtgg cgctgttgct
    13621 gtgcgacaac atcggcgaag cgcgcgtgct ggcaaatgaa ctcgatgcgc taaaccagac
    13681 gcgaaaagag atcgaacaag gaatgcaagt tgaagccctg accctgtgcg agaaactgga
    13741 gcgaagtcgc gacacgctac ccggcgggct ggcaatgtac caccccgaat ggcatcaggg
    13801 cgttgtcggt attctggctt cgcgcatcaa agagcgtttt caccgtccgg ttatcgcctt
    13861 tgcgccagca ggtgatggta cgctgaaagg ttcaggtcgc tccattcagg ggctgcatat
    13921 gcgtgatgca ctggagcgat tagacacact ctaccctggc atgatgctga agtttggcgg
    13981 tcatgcgatg gcggcgggtt tgtcgctgga agaggataaa ttcgaactct ttcaacaacg
    14041 gtttggcgaa ctggttacag agtggctgga cccttcgcta ttgcaaggcg aagtggtgtc
    14101 agacggtccg ttaagcccgg ccgaaatgac catggaagtg gcgcagttgc tgtgcgatgc
    14161 tggcccgtgg gggcagatgt tcccggagcc gctgtttgac ggtcatttcc gtctgctgca
    14221 acagcggctg gtgggcgaac gtcatttgaa ggtgatggtc gaaccggtcg gcggcggtcc
    14281 actgctggat ggtattgctt ttaatgtcga taccgccctc tggccggata acggcgtgcg
    14341 tgaagtgcaa ctggcttata agctcgatat caacgagttt cgcggcaacc gcagcctgca
    14401 aattatcatc gacaatatct ggccaattta gcgtcatctt ctctataaaa aagagcgtgg
    14461 attgggtaca atcccgctct tatcaccgca ttttgactag ctcaataaaa gaaatcagac
    14521 catgtttgaa attaatccgg taaataatcg cattcaggac ctcacggaac gctccgacgt
    14581 tcttaggggg tatctttgac tacgacgcca agaaagagcg tctggaagaa gtaaacgccg
    14641 agctggaaca gccggatgtc tggaacgaac ccgaacgcgc acaggcgctg ggtaaagagc
    14701 gttcctccct cgaagccgtt gtcgacaccc tcgaccaaat gaaacagggg ctggaagatg
    14761 tttctggtct gctggaactg gctgtagaag ctgacgacga agaaaccttt aacgaagccg
    14821 ttgctgaact cgacgccctg gaagaaaaac tggcgcagct tgagttccgc cgtatgttct
    14881 ctggcgaata tgacagcgcc gactgctacc tcgatattca ggcggggtct ggcggtacgg
    14941 aagcacagga ctgggcgagc atgcttgagc gtatgtatct gcgctgggct gaatcgcgtg
    15001 gtttcaaaac tgaaatcatc gaagagtcgg aaggtgaagt ggcgggtatt aaatccgtga
    15061 cgatcaaaat ctccggcgat tacgcttacg gctggctgcg tacagaaact ggcgttcacc
    15121 gcctggtgcg taaaagcccg tttgactccg gcggtcgtcg ccacacatcg ttcagctctg
    15181 cgtttgttta cccggaagtt gatgatgata ttgatatcga aatcaacccg gcggatctgc
    15241 gcattgacgt ttatcgcgcg tccggcgcgg gcggtcagca cgttaaccgt accgaatctg
    15301 cggtgcgtat tacccacatc ccaaccggga tcgtgaccca atgccagaac gaccgttccc
    15361 agcacaagaa caaagaccag gccatgaagc agatgaaagc gaagctttat gaactggaga
    15421 tgcagaagaa aaatgccgag aaacaggcga tggaagataa caaatccgac atcggctggg
    15481 gcagccagat tcgttcttat gtccttgatg actcccgcat taaagatctg cgtaccgggg
    15541 tagaaacccg caacacgcag gccgtgctgg acggcagcct ggatcaattt atcgaagcaa
    15601 gtttgaaagc agggttatga ggaaccaaca tgtctgaaca acacgcacag ggcgctgacg
    15661 cggtagtcga tcttaacaat gaactgaaaa cgcgtcgtga gaagctggcg aacctgcgcg
    15721 agcaggggat tgccttcccg aacgatttcc gtcgcgatca tacctctgac caattgcacg
    15781 cagaattcga cggtaaagag aacgaagaac tggaagcgct gaacatcgaa gtcgccgttg
    15841 ctggccgcat gatgacccgc cgtattatgg gtaaagcgtc tttcgttacc ctgcaggatg
    15901 tgggtggccg cattcagctg tacgttgccc gtgacgatct gccggaaggc gtttacaacg
    15961 agcagttcaa aaaatgggac ctcggtgaca tcctcggcgc gaaaggtaaa ctgttcaaaa
    16021 ccaaaaccgg cgaactgtct atccactgta ccgagctgcg tctgctgacc aaagcactgc
    16081 gtccgctgcc ggataaattc cacggcttgc aggatcagga agcgcgctat cgtcagcgtt
    16141 atctggatct catctctaac gatgaatccc gtaacacctt taaagtgcgc tcgcagatcc
    16201 tctctggtat tcgccagttc atggtgaacc gcggctttat ggaagttgaa acgccgatga
    16261 tgcaggtgat ccctggcggt gccgctgcgc gtccgtttat cacccaccat aacgcgctgg
    16321 atctcgacat gtacctgcgt atcgcgccgg aactgtacct caagcgtctg gtggtcggtg
    16381 gcttcgagcg tgtattcgaa atcaaccgta acttccgtaa cgaaggtatc tccgtacgtc
    16441 ataacccaga gttcaccatg atggaactct acatggctta cgcagattac aaagatctga
    16501 tcgagctgac cgaatcgctg ttccgtactc tggcacagga tattctcggt aagacggaag
    16561 tgacctatgg cgacgtgacg ctggacttcg gtaaaccgtt cgaaaaactg accatgcgtg
    16621 aagcgatcaa gaaatatcgc ccggaaaccg acatggcgga tctggataac ttcgactctg
    16681 cgaaagcgat tgctgaatct atcggtatcc acgttgagaa gagctggggt ctgggccgta
    16741 tcgttaccga gatcttcgaa gaagtggcag aagcacatct gattcagccg accttcatta
    16801 ctgaatatcc ggcagaagtt tctccgctgg cgcgtcgtaa cgacattaac ccggaaatca
    16861 cagaccgctt tgagttcttc atcggtggtc gtgaaatcgg taacggcttt agcgagctga
    16921 atgacgcgga agatcaggcg caacgcttcc tggatcaggt tgccgcgaaa gacgcaggtg
    16981 acgacgaagc gatgttctac gatgaagatt acgttaccgc actggaacat ggcttaccgc
    17041 cgacagcagg tctggggatt ggcatcgacc gtatggtaat gctgttcacc aacagccata
    17101 ccatccgcga cgttattctg ttcccggcga tgcgtccggt gaaataagca ttacgttatg
    17161 ctcacaaccc cggcaaatgt cggggttttt tatttaagct gggtaaatgc agataatcgt
    17221 attctggctt cgcgatttgt cgcctgcatc accatccacg gactgaacgc ccacggcgtg
    17281 gcatcaatac cgtgtaatac atcagctaaa tcacaccatt gataatccat cacttcatca
    17341 tcgttgatct gtaacgcact ggtggtgcgt gcggcaaata ccggacacac ttcattttcc
    17401 acaatgccac tcggatcggt ggcgcggtag cgaaagtcag gatagataga ttcaggaggc
    17461 gtaatttcca cgccaagctc ataacggcaa cggcggatca ccgcgtcttc gctgctttct
    17521 cccagttgtg ggtgcccaca aaccgagtta gtccacacgc caggccacgc ttttttgctc
    17581 agtgcgcggc gggtaactaa taattgcccc ttggcattaa acagccaact ggagaacgcg
    17641 agatgtaaga gggtgtctgc cgtgtgtgcg gcatactttt ccagcgtacc agtgggaact
    17701 ccctgtgcat tcaataaaat gacgtgttcc gtttgcataa tttctcacat gtaattctga
    17761 tcactcatca ttatagataa ttgaagagtg aaatgattaa ccaagtgagc gtttatcgac
    17821 aaccgcccgt tttgagcgga tgccgacagg taaaaaccat ttaaaaaagt agcatcatat
    17881 atttgaagcg actttcaatg gccattgcaa cacagtcaga aatgacagga atagagagag
    17941 taaaaagcaa cagcagactg aatatagcgc gcattaaaaa attcatatta ttctccatgc
    18001 tcatttttct tcagcttgcg catcagaagc cacatacgta ctgtcctgac ttttaaatct
    18061 ttatcagaga cggattcctt caccaggtca gcatgatgct ggtagccatt aaagaagaca
    18121 ttcagcacaa cggcgctaag ggttgcgagc ataatgccgc tatgcagcaa cggttgcagt
    18181 acggccggta acttagaaaa gaaatcgtga gagagcgtcg gagtcatgcc aacgccgaga
    18241 ctgatcgcca caatatagag gttgtaacgg ttggtggtgt agttacagcg cgacagaatt
    18301 cgaatccctg tcgccagtac catgccgaac atcaccagcc cagcaccgcc cagcacaaat
    18361 tgcggaatgg aggcgaccag caccgccatt tttggcacca tgccaaacag gattaatata
    18421 attcccgaag aaatacacac ccagcggcta tgaacgcgcg tcacgctaac caggccaacg
    18481 ttttgtgaaa atgacgtgtg ggggaagctg ttaaacgttc cgcctatcat tgtccctacg
    18541 ccatcgacac gcagcccgcg aataatatcg tgtgaagaga gtttacgacc gactatttca
    18601 cccagtgcca ggaacatccc catcgactcg ataaacacga tgattaacac ggcagtcatg
    18661 gtcagaatgg aaacgggatc gaaaatcggc ataccaaatg acatcggcgt gacaatcgca
    18721 aaccatgaag catcatgtag cccggataaa ttgacttcat tcatcatcca cgaaagtaaa
    18781 aagccaaata caatccccag taatacggcg acgttggaca taaatccttt cgcatagcga
    18841 gtaatgagca agataaaaat taagacagca aaggagatac ctaaataaac gggattacca
    18901 tattgcggat tccctttacc tccggcggcc cagtcaatac ccacctgaat gatgctaagc
    18961 ccaatagaag taataaccac accggtaacc agtggcggga ataaaggcat caagcgaccg
    19021 ataagtggcg ctaataatgt ggtgataaaa cccgcggcga tagtggcacc aaatatcccc
    19081 agcaggccga tatccgggtt catcccaatg gctatcattg gtgttacagc agcaaaggtc
    19141 accgacataa tcaccggcag gcggatcccc ataaagcggc cgataccgat acattgcaat
    19201 aatgtgacga tcccgcagca aaagagatcc gagctaatga gcatcgcaat agcttctttt
    19261 gagaggccca gtcggtcacc aatcataaga ggaacagcga ctgcacctgc gtacatgaca
    19321 aggacgtgtt gcagaccgag tatgatcagc tttcctggtg ataatatgcg gtcaacctca
    19381 tcagttgggc ggtcttgccc agaagatgag ggaagttggg aatctatggc gctcatcagt
    19441 gtttctcctt tcgcccgcca gagtaaaaag caaccgttta cgttaatgaa actgtctaaa
    19501 cacatgctcg tggagtgcca gtttttgcga ggaaggggag ttagagacag gaattgcgga
    19561 ggcaatcaca taatcgtggt taaaccacga aaatgagaag agaacctgac aatgaaatta
    19621 tctgtaattt tctcatattg atatttcatt aatcattctg gcatgtcgca ctctcgcatt
    19681 taatcgtttt tatctggata gcgctctttt ggtcggcaat gttgaaataa tgaaagcggc
    19741 atagattgag gggcaataat ttgaactcag tcgcaaaatt ataattaaaa cggtcatgct
    19801 acctaatata tgcccgttta ttgcatcgcg aaatttgaga agacataact cattaaatga
    19861 aagggatgca aatgaataag tttatcgctg ctgaagctgc ggaatgtata ggctgtcatg
    19921 cttgtgaaat tgcctgtgcg gtggcacaca atcaagaaaa ctggccgctg agtcacagtg
    19981 actttagacc gcgtatccac gttgtaggga aaggccaggc tgcgaatccg gtggcctgcc
    20041 atcactgcaa caatgcccct tgcgttacgg cttgtccggt taatgctctg actttccagt
    20101 ccgatagcgt acaactggac gagcaaaaat gtattggttg taaaagatgc gcaatcgctt
    20161 gcccctttgg cgtcgttgag atggtcgata cgattgcgca gaaatgcgac ctttgtaacc
    20221 agcgcagttc tggcacgcaa gcctgtattg aagtctgccc aacacaggcg ttacgactga
    20281 tggacgataa agggttacag tggataaagg tggctcgcca gcgcaaaacg gcagcaggaa
    20341 aagcgtcatc agacgctcag ccatctcgca gtgcagcgtt gctccccgtt aactcgcgta
    20401 aaggcgcaga taaaatttca gcgagtgaac ggaaaaccca ctttggcgaa atctattgcg
    20461 ggctggatcc actgcaagcg acttatgaga gtgaccgctg tgtttattgt gccgaaaaag
    20521 ctaactgcaa ctggcattgc ccgctgcata acgctattcc ggattacatc cgtctggtac
    20581 aggaaggaaa gattattgaa gcggcagaac tttgccacca gaccagttcc ttaccagaaa
    20641 tctgcggcag ggtatgccca caggaccgtc tttgtgaagg cgcatgtact ttgaaagatc
    20701 actctggcgc agtctctatc ggtaatctgg aacgctacat caccgatacc gcgctggcga
    20761 tgggctggcg tcccgatgtc agcaaagttg ttccccgtag cgaaaaagtg gcggtgattg
    20821 gcgctgggcc agcagggtta gggtgtgctg atattctggc gcgcgcggga gttcaggttg
    20881 atgtctttga tcgccatcca gaaattggcg gtatgctgac ttttggcatt cctcctttca
    20941 aactcgataa aacggtatta agccagcggc gagagatatt caccgcaatg ggaatcgact
    21001 ttcatcttaa ctgtgaaatt ggccgcgata ttacctttag cgatttaact tctgaatatg
    21061 atgcagtttt catcggcgtg gggacttacg ggatgatgcg agcagatctg ccgcatgaag
    21121 atgcacccgg tgtcattcag gctctaccgt tcctgactgc ccatacccgc cagctcatgg
    21181 gattgccgga gtctgaagag tatccgctga cggacgtgga aggtaagcga gtcgtggtat
    21241 tgggcggtgg cgatacgaca atggattgtt tgcggacttc cattcgcctc aatgctgcca
    21301 gcgtgacctg cgcgtatcgt cgtgatgaag tcagtatgcc gggctcgcgc aaagaggtgg
    21361 ttaatgcgcg cgaggaaggt gtcgagtttc agttcaatgt tcaaccgcaa tatatcgctt
    21421 gtgacgaaga tgggcgttta actgcggtgg gcctgattcg taccgccatg ggtgagccgg
    21481 ggccggatgg tcgccgtcgt cctcgtccgg tagcgggttc agagtttgaa ttgcccgccg
    21541 atgttctgat tatggccttt ggtttccagg cgcatgccat gccgtggttg cagggcagcg
    21601 gaattaaact cgataaatgg ggcctgattc aaaccggtga cgtcgggtat ttacctaccc
    21661 agacgcatct gaaaaaagtc tttgctggtg gtgatgcagt tcatggcgcg gatctggttg
    21721 tcactgcaat ggccgcagga aggcaggcgg cgcgcgatat gttaactctg tttgatacga
    21781 aggcatcgtg atgaaatcgt taattatcgt taatccggct gactgtattg gctgccgtac
    21841 ttgtgaagta gcctgtgtgg tcgctcaccc ttcagaacag gagttgaatg ccgatgtctt
    21901 tttaccccgg ctgaaggtac agcgactgga cagcatcagt gcgccggtga tgtgccatca
    21961 gtgtgaaaac gccccttgtg ttggcgcttg ccccgtgggg gcgctgacga tgggcgagca
    22021 ggtggtgcag accaattctg cccgttgtat tggctgtcag agttgcgtca gtgcgtgccc
    22081 gtttggaatg atcaccattc agtctttgcc gggggatacc cggcaacaaa tcgtgaaatg
    22141 cgatctctgt gaacagcggg aagaggggcc agcctgcgtt gaatcttgcc caacacaggc
    22201 gctgcagttg cttactgaaa gagaactcag gcgagtccgc cagcagcgta ttgttgccag
    22261 cggtgagaat cctctgtgaa agaaatctcc gcgctgaaag gcgcggagaa tctattagat
    22321 tgcccaacca cccgcgtaaa atgcgaccag tgcggcagta atgatcaccg taccaatatt
    22381 cagcttttgc cattcgcgtg caaagacgcg gcctacgacc agtgtcacaa agcccagcat
    22441 aataccggta acgatattac aagtcagaac gatgaacacg gcacacacca ggccagccat
    22501 agcgtcaata aaatcattga aatccagctt cgagacgtta cttaacatca gcaaacccac
    22561 gtacatcaga gcgggtgcag tggcgtaacc agggatcaaa aatgacagcg gtgataagaa
    22621 cagaatcagc aggaataacg cccccactac ggttgcggtc aaccctgttt tacccccggc
    22681 ggcggttcct gccgctgatt cgatataaac cgctgcgggc gctgcgccca ccagaccgga
    22741 gaatattgaa cttactgagt cactggtcag ggctttgccg ccgttgatga tctggttgtc
    22801 tttatccaac aaattcgcct gaccggcgac ggcacggatg gtgccagtgg cgtcgaacac
    22861 tgcggtcatc accaatgcca gtacactcgg aagtacggtt ggctggagtg cgcccataat
    22921 atcgaggctg aaaatcagag acttaccatc ttcgccagtc agacttggca tcgccaccag
    22981 accgtggtat ttcaccgctg ggtcaaagat taagccgata atcgaaattg cgataatcac
    23041 caacaagatc ccgccgggta cgcggcactt ctccagaccg aagatgaccg ccagccccag
    23101 caagctcatc atcaccggga aggaggtaaa cgcaccgagc gccactggca agccttcaat
    23161 cgggtttttg ataaccatac ccacaccgtt agcagcaatc agcagcagaa acaggccgat
    23221 accgatacct gtaccgtgag cgatacccat cggtaaatta cgtaagatcc aggtacgcac
    23281 accggttacg gaaatagcgg tgaagatgac gcccatcaga aagaccgcgc ccagtgcgac
    23341 aggaacgcta atttgttgcc cgagtaccag actgaatgcg gtaaatgccg tcaaggaaat
    23401 cgcacaacca atcgccattg gcaaattggc ccacaacccc atcagcaacg agccgaagcc
    23461 cgcgaccaga caggtggcaa caaacacagc tgcgggagga aaacctgctt tgcccagcat
    23521 tcccggaacg acgataacgg aataaaccat ggccagaaag gtcgttaagc cagccagtac
    23581 ttcctgacga acggtactgc cacgagcggt aattttaaaa taattatcca gcgcgccctg
    23641 tggctttggt gcgtccggtg tttgtaggat gtctccagac ataatgatgt cctcacgagg
    23701 atgttaaagt tttattatta gttgcgttcg tacaccagac gaccatcaac gtaggtgcgg
    23761 tagatcgaac ggtcatcgcc caacgtcatc atcacgaaca atttgtcgac taaagaaaca
    23821 gagttgtcat agcgcagctg ctgtagcgga gtggcggtgg gttccatcac cacgaaatca
    23881 gcctctttgc caggtaaaaa gttgccaatc aaatcgtcaa ggcccagaga tttcgctccg
    23941 ccgagcgtgg ccaggtaaaa cgcttcatat gccgagaggc gatagccttg taattgcaat
    24001 actttgtagg cttcgttcag cgtttgcagc atgttgaaag tggttccggc accgatatcc
    24061 gttcccatgc ccactttaac tttcttctgc catgcttttt tcaagttgaa taagccgctg
    24121 ccgaggtaaa ggttggaggt cggacagaaa gcaatgctgg atttggtttc gctgagacga
    24181 tcccactctt tttcttcgag atggacgcag tgagcaaaga cacagttttt accggtcagg
    24241 ccgtactgat ggtaaacatc cagataacca tcatggtcag gataaagcga tttcacccag
    24301 gcaatttcat ctttgttttc acagagatgg gtatgtaccc acgtatccgg atattcttct
    24361 ttcaggcgtt gcgccatcgc catctgttca ggagatgagg tcggggcgaa gcgtggcgta
    24421 atcgcatata gcagacgacc atttttgtgc cagcgttcaa tcagttcttt gctttggtga
    24481 tagctgcttt cggcagtgtc gagcagataa tccggtgcgt tgcggtccat catcacctta
    24541 ccggcaatca tacgcatatt gatatgactg gcggcttcaa acagcgcatc aacagattgc
    24601 ggatgaacag tgccaaacac cagcgccgtg gtggttccgt tacgtaaaag ctgcttgatg
    24661 aagaatgccg acatttcgcg ggcgtactct aaatcctcat aacgacgttc agtagggaag
    24721 gtgtgtttat tcaaccactc cagcaattgt tcaccatagg cccccaccat ttcactttgc
    24781 ggataatgga tatgtgtatc gacaaagccc ggtactatca gtttgccgcg atagtcgcgc
    24841 acgcgaatag tgtcaggaat ttgatgcttt ccgtcttccc attcgccaaa ccattccact
    24901 tttccctgtt taatgagtaa taaaccatcc tcaataaacc gcagcgcaga ggcaatctct
    24961 tccgggttat cgaccgtacg ggtgacatca ataaaactgc ctcgtaccgc tttcaacgtg
    25021 tgttctcctg acatcattga ctcctttact gttaatccat ctcttccgct gaggtaatac
    25081 caggcagaac gttttcctgt cggtagccac cagggaggat aatatttaat aaaatcgcag
    25141 ttaacccgcc agcacaaata gggttttcaa ctagtacata aatagaggct ggcaatattt
    25201 taaaaatttc gggatcgtag gagacgccaa gtcctaaacc taaagaagtg gcgacaataa
    25261 gtgtttctcg gcgctttaaa ccgttggtga tgattatgcg aatcccagcg atggcaatca
    25321 tggaaaacat caacgtcatt gcgcctccca gaactgccga gggaatggtc gtgaagaagc
    25381 cgccaatcat cggaaacaaa ccgaggataa ccagcattac cgcgatggtt cgcccgacat
    25441 aacgtgaagc gacgccagtc atctgaataa ccccattatt ttgcgcaaac gtggttaagg
    25501 gtaatgaccc gacagcggag gcgataacag aaaccagacc gtctgccagc acgccgcctt
    25561 tcagtcggga ctgatactct tctccctgaa tgggtcggcg ggaaaccatt gccgtggcgg
    25621 tgatatcgcc gactgcttcc agcacgctaa gcagataaat cgtgccaacc accaggaact
    25681 gatggaagct aaaactaaag ccgtatttga acggatgcgg gatggtgatt aacggcaaat
    25741 tgcgcatact gctgaaatcc accatgccca ggcataacga tgcgatatag ccgacacata
    25801 gcccaatggc gatccctccc atgcgtagca acggactgcg acagcagtta aagccgatca
    25861 ccacaattaa aaccaataaa ccaacgccga gatgttcgta attgccgaac gtaccgctgc
    25921 ttttggctgc aaatcctcca ccaaaatcga taatgccgac tttaatcagg cttaagccga
    25981 tcatcagtac cacaataccg ctgacggtag gcgtaataac ccgacgtaaa tagggcaaga
    26041 taaatgaaga tccgacaacc agaaatgcgc caacgaagga gacgccgaga agcgacgaca
    26101 tgattaactc ttcgtgaaaa ccgtcgcttt tcatgctgct gcccagcgca atcatgaccg
    26161 taacaaatga aaaattgact gactgaattg agagtaggcc agaaccgacg atgccgtagc
    26221 ggtttacttg taaccaggta ccaataccag aggcgatcat cgccatagaa acaagatagg
    26281 cagttgtttc agcggaaagc tgtaaggccg cccccacgat taacgcgggg gttaccatcg
    26341 gaacgaaaat tgccaacaga tgggtaatgg caccaacgag agcctgatga aagggagggc
    26401 gatcctccag ttcaaatata aggtcagaac ctgcattgtt tatatcagac atcccttcac
    26461 tccttttaaa gtaaatttga tcatctgaat tagtaaaata aattcccacg aatttggggc
    26521 gcaggacgcc ttgagaacgg cttatctcat tttcgcagtc actatttagt catcgtccaa
    26581 acagagaaac aggccgccga agcggccatt tattatttat attttttcca gcgcagtgag
    26641 tattttctcc ggtgtgaaat gccattcgcg taaccagatg ccgcatgcat cgtgaattgc
    26701 ggtagcaatc gccggagctg cgccatttac accgatttcc gagatcgatt tcgccccgaa
    26761 cgggccgact ttatcgtcgc tcggcaccag cacagcgcgg aaatcacgcg gaatgtcacc
    26821 aattttcggt gcgccgtaac tgcgtaaatc acgcgttaac gggtgacctt cggcgtcata
    26881 aatgatctct tcgctcatac tgtggccgat agctcgcagg gtagcaccgt agatttgtcc
    26941 caatgctaat tccgggttga ccggtgtacc gcagtccagc aaggcgtaga atttatccag
    27001 gcggatttca cccgtacgcg tgttgacggc aacttcagcg aagtttgcgc catacgggaa
    27061 ggcgaaatca ggcgtgatat aactgccagt tcccaccagt gaaccaaagc cggtgccggt
    27121 ttcgccttta tgagcaatat ccccgaagct gacttcgcct ttcttgccgc gcacgacgcc
    27181 cggcgttgcc agttgaacat ctgccactgc ctcacccaac atttgcgcgc cgtggaacag
    27241 aattttctcc cgcagatttt ccgctgccaa acgcgccgcg ttacctgaga agcaagtacc
    27301 ggacgaggca tatgcgcctt tatcaaacaa cgcatgatcg gtatcaccgg agataacatg
    27361 cacgtcctgc ggtgggcagt gcagcacttc tgctgccagt ttcgtcacta cagtatccag
    27421 accagtacca atatccgcac cgccagaatg aacgataaag gtgccgtccg attccagttt
    27481 gatcatgcag ttagcctgat cgatatccgg gatacccgat ttctgcatga taatggcgac
    27541 gccgcgaccg atgtgccagt caccattttg cggtttcggt gacgaccatt ggatcatctc
    27601 gcgaccctga cgcaggatct cttccagtgc acagctggcg gcggaaggaa cggaggtcgg
    27661 cgctttacct tcaccgattg caccgagaat tttcagctct tgcccttcgt gtacccggtt
    27721 acgttcgata atttccagtt ggtcgatctg taactgttca gccagttccg ctaatgccat
    27781 ggtgatagcg aagttacctt tcggtgcgcc ataaccctga taagcaccat ttgggcaaat
    27841 gttgctgtag taggtggtga cctggaaatc gacgttatcg cacggatata acggcagcga
    27901 cagcgccggt ccgttacacg gtacggtgag tgagtggttg ccgtaagggc cagtgttggc
    27961 gcggaaatcc atcttcactg ccgtcaggcg accatctttt ttcgctccca gtttgacggt
    28021 gactttcgcg acgtgacgag aggtgttagc aataaactct tcttcacggg tgtagcggaa
    28081 cagtaccgga cgcccggtca cgcaggttgc ccaggcgcac acttcttcca gcaggatgtc
    28141 ctgtttggaa ccaaaaccgc cgccaactcg ctctttaatg acatgaactt tatgctgttt
    28201 catgcccacg aggcgcgcga cctggcggcg taagtgccat ggtacctggg tggaggcgtg
    28261 gataaccaga cgatcgccgt ccatccgggt aaagcagata tgtgtttcag tcgggcactg
    28321 ctgtgcctgc gttgagttat aggttcgctc aatgatcaca tcggcatcgg caaagccttt
    28381 gtccatatcg ccaatatgac catgaatact ggcggcgata tttttgcgag ggcgagaacc
    28441 gatcgggaag ttgatgatca tatgctcgcc gcgctgggct gcatggctgt tatcgtcttc
    28501 cagagtatct ggcacaccag caacatacac caccggttca tcgtgcacga caggcgcatc
    28561 ttccgccatt gcttcgtcga tcgacattac cggcttaagc acttcatatt caacgtcgat
    28621 gagcttcagt gcttcgagcg caatttcttc actttctgcg acgaccgcag caacacgatc
    28681 gccgacgtga cgcattttct tgccgaacat acggcggtca agcggtgacg gttccggtgc
    28741 gctctgacca cccggggtat agtagatatc cgggcaattc aggtgagtaa taacgtgaac
    28801 gacgcccggt aaggcttcag ctttgctgac atccagatga gtaatcagtg cgtgagcgtg
    28861 tgggctacgt aacattttaa tgacgcaggc gtcagccgtt acgcggtctt caacatagca
    28921 gggtttcgcc tgcaccattt tcgcggcatc agttttagga taatgcttac caatgacttc
    28981 taggtcgtca cggaaagtcg gagcgatatc aatggtggcc tgcggattat ttttacgtgc
    29041 aaccgccagt tcaatgacct gatagtattg ctgccagcca gcatcgcggc tgaacaaacc
    29101 agaaagcgcg tcgtcgatct cttcgcgggt aggtgcggcg atgcgatcga gaagatcggt
    29161 gataatcaga gctgcagctg gatcgttata accagactgc accacgccaa catcaaccat
    29221 tgcctgttga accagactta actcgttcca tttgcccaga gattctgcgg tacgaatatc
    29281 tgccttctct aactgtgcgg caataagcaa ggacgcgtta acgatattac cgttaaagat
    29341 tattgcgtca gaaccagcaa acccgaagcc atcatcactg ttgcgtacag agtgcattcc
    29401 catgttaaac aacagctttt gcacgttttc gcctggatta acggttagct cctgaggcgc
    29461 gccatttaaa gtaaagtgga tgatcatacg gcttcctcct cagcctgttg gcagtcggca
    29521 tacaggtcgg ctaccagaac tcccgagata tagcgtttat aggccacgct gccccgcaaa
    29581 tcttcctgcg ggaaaatggc gttggcgaca gcctgttcaa gtgcattgcc ttccagattt
    29641 tgtttttcga catcatgcag acgcagtgct ttactggcga cgccatccag cgcaattcgc
    29701 ataccgtcgt ggtctgtcat tgcaacggct gccgtcacga cggttaaacc tgcctgagaa
    29761 cggctaattt tgcgggtcgc acaggtgcga tacggatctt taatgataat ttcggttagc
    29821 aggcgatcgc atgggcaggc caggtagtcc tcgattgaca gcgtttcgcc gttgccaaaa
    29881 accagttcag catccagtgc caacaggacg ggaagcagca ccgactcttc ctggcgggcg
    29941 gcgatttcac caccaatggt cgactgatta cgaacatggc gtgagtaaac aaaaccgagg
    30001 gcttcacgca gcgctgcagg aataaatcgc gcatcacgca gtggctgcaa gcgagacatt
    30061 gcgccaatcc gcagtgcacc gttatcccag tcaacccagt ccagttccag atcctgtaag
    30121 gaaatggcaa tctttttatc ggtacgggtt ggtgtagcat tgagtttgct gcccccggcg
    30181 aaccagacgg cttcatcctg gtagcggcgc ttcagttcca gcgcctgttc gacagagtcg
    30241 ggcctgaaaa attgttcaat catcttcttt ccttatttaa ccctcttccc tgcgggaaga
    30301 gggcatctgt catttatgcc agcgcatcca tccgacgcca catactggca gcggcttttc
    30361 tggcttgtgc ataaatggaa tcgcaatcga agttaaactg acggtcttca tagaccatca
    30421 caccattgac cattacgctg tgaacgctgc ctgagcccat accgaaagcg atatgcccgg
    30481 cgatattgtc tgccagcagc ggcgtcggcg agttgtaatc gcaaatggtt aaatcagctt
    30541 tgtaaccggc ttccagaagc ccaaatttcg cgccaaagtt gcggctcatc agttcgttgc
    30601 cattagtcag ggctttggca aaactgtcag gccacagcgg accacccgca tcgcgatgtt
    30661 taaagaaggc aaatttcatc tcttcaaaca tgtccgaacc aatgccgtcc gttcccaacg
    30721 ccagattgcg gatgtcgcta aggtgatggt tgtagccgac atggttgttc atgtttgaac
    30781 gggcgttatg caccaggaac gcatcgcgct gattgagtag ggtgatgtca tctttcgaca
    30841 agtaaagccc atgagcgacc agcgttttgc tgtcgatgag atcgaattgc gctagtcgtg
    30901 ccagcaggtc tttgccgtac cagtggtgac tgtaggaaac gtcgtaaagg tcttccgcag
    30961 cgtgaatatg caaaccacgg cctgtggctt tcacggcttc acgtagcatc tccagaccgg
    31021 catctggcac ggtaaacggc gcgtgagcac cgatatgtgc ttccaccaga tacggctcgg
    31081 ttgctgcttt cttcgcctca tcaatctgac gggcgaagcg gatgttttct tctacacctt
    31141 cctgcaactc tttgatgccg ttgttacggt cagtagtttc aaaacaggtc atcgcgcgca
    31201 ggccaacttt taaaaatgcg tcgcgcaatg tggagagcga cccgccgata tacgccggag
    31261 aggcatggtg atcgataacc gatgtacatc cgctcttaat cgcttccagg gaacaaatca
    31321 gcccgctgta atagagcgac tcttcatcaa gggcgcgatc gagccgccac cagagatttt
    31381 tcagcgttga gatgaaatcc gggcaggggg cgatgtttgc cataattccg cgggaaagcc
    31441 ccgagtaaaa atggttgtgc gagcagacaa ttcccggcat cacaatccga ccatgcatct
    31501 ctttgtagct ggcatcgggg tagcgttgcg tcagggcatc gccgatagcg acaatcacat
    31561 cattttcgat ggcgatatca acgccttcct gcactttcgc cgggtgtaac tgtactgcgg
    31621 tgacattctt cagaatcaac atgattacac ctccacgcgg cccagcagat aatgatgatg
    31681 ctggtggaca tggctgatga tgcggcacat atcgttcagc tccggtggta cgttgtcgaa
    31741 ctggccttcg ctgtcgatgt tcagcaccaa gctttggtta ttcagacgta cgcgtacccg
    31801 acaatcttcc accaggaagc ctgggttgct gctgttatca aagtcttgcg acaggctgaa
    31861 gacggtgatt ttgtctttgt acggtttacc gttccacgga cagaactgag cgcagttgcc
    31921 gcattcgtta cagtaagcgt cgaggtgcag cgtctggaaa cggttctgga agcctgggac
    31981 cgcaatggag acgttggcgc ggttcgggca gacatccaca cacttgctgc aaacgtagtt
    32041 acattcgagg cagcgagcgg cttcctgcgc gacaaacgca tcacgatcgt cactgttcac
    32101 cagcgtgata gagatatcgc ctttacgttg atagatttcc gccggattga cgttgttcca
    32161 gtatttattg ttctggtggg aacggatatt ttcccgacta aggatggcat cggtcgcccg
    32221 acgcgcggtt ccgacagcag cgacaatgga ggatggtccg cgctgtacgt cgccgatcat
    32281 aaagacgtca gtcagacgag tttcgccatt atggtcgacg tctggccagc cgtttttgtc
    32341 cagcggtacg cccatcgcat tcagggcttc agtatcctgc tgttcaccga tggcggtgat
    32401 cagactgtct acatgcagtg ttactgtttc gttggtttca accggacggc gacgaccttt
    32461 ctcatcgggt tcgccaagcg acataacgcg caaggttaag gtgccatcag catcgaaaca
    32521 ttccggatta ttcaggaaac ggaactccac gccgtcgtgc aacgcttctt catactcttc
    32581 gcgccatgcg ggcatctctt gcagtgaacg acggtaaacg atcgttgctt tttctacgcc
    32641 tggaacgcgt aacgccgcac gagcgcagtc cattgcggtg ttacccgccc cgacaacgac
    32701 cacatgtttg cccagcttga gcgctgtacc cttgttgtat tcacgcagga aggggagtga
    32761 tttccagaca ttttggttgt cgcccgccag tttcacaccg ctatttttat cagtgccggt
    32821 ggcaatcaga acatagtgga agccctgatt ttttaactgt tcaacggtca aatcgggtga
    32881 gcagccatac tcaaatttca cgccgtgagc ggcaacaaaa tcgatatcgt gctgaattaa
    32941 ctctgcagga atacggaact gaggaatgat atttttcacc acgccgccag cattggcttc
    33001 gcgttcaaac agcgtaaccg gatggcccgc tctggcaagg aagtaacctg ctgccagacc
    33061 cgccggacct gcaccaatca cggcaactgg atggcgtgaa ccagaaccgg ctggtttgtg
    33121 ccagcgttgc ttatattcat cccaaccttt ttccagcgcg acttttttca gttcgcggat
    33181 attcagcgca ctgtcgtaat ccaggcgggt acagttgtat tggcactggt gatcgcaaat
    33241 atgaccggta atggcgggca gagcgttgcg ttggtagatg agttccagcg cgtcggcata
    33301 gcggtgttcg ccaagcagac ggatgtattc cggaatatct tgcttgatag cacaggcagt
    33361 aacacagggg gcaacgtagc aatcggtcag cggcaggtct tctgccactt caatacgctc
    33421 ttctggcttc cagtgtttct gggtgtattc catggttaac gcatctgctg ccagtctgtt
    33481 cagtcgttcg acgtcaacat ggtcaagccc ccaggcgtcg gagccttcca gctcgcgcat
    33541 gcaggcactt aagcgcagat agccgcctgg tttcagcagg tcggttgcca tagtaatagg
    33601 gcgaatacca gtatcaaaaa tatcgcggat agtcagctga ctggcaccac cggaataaga
    33661 aatgggcagt ttgccgtcaa aggcgcgaga gagaactgct gcaacattga tggagagcgg
    33721 gaacagcgca cggcctgaca tatacatctc ttcaccaggc agtgcgcctt tattgttgat
    33781 ggtgccgaga gtgttagtca gttttacgcc aaagccgagt gatttttctt ttgccagtgc
    33841 catcaggcgt tccagcattt ccagtgcttg cgtcagcttg aggtcgtgat caaatgactc
    33901 ttcttttaag cctatgtaac cgaaaccgca gacatcgagg atctcacgaa cacgcgcgta
    33961 tcccagtaag gtcgggttaa gtttcacaaa ggtgttgagc cctttttctt ccagcatgta
    34021 gcggcaaatg gcttcgattt catgcggagg acagccgtgc atggtggaga gggtgacgcc
    34081 atgcaccata ctggtgggga tgcgagcggg taaggcttgc aagctttcgc gtttttcctg
    34141 caatccgtgg cgggctaaaa atgcgtcatc ctggagtaat ttattcagcg tatcgcgata
    34201 ttgagcgaat ttcggatggt cagatgcgtc catcatattg tcgatgaact gttgcatcgg
    34261 cggttgctta ataccttcga ggttgtaacc gacgctcata ttaaagatga acgatttacc
    34321 ggaatcagaa ggctggaaca tcgcttcgag aaggtgcagg gcaaaccagg ctttgaggta
    34381 ttcatcccag gctttaagca gggtaaattc ggtagaccat tcggtgttaa aacactcgtc
    34441 ttcggcatcg atacagggct tttccagctc caggcggtca agaatttgga cggtttttag
    34501 ttcgatgaag cgtccgccag tcagccagga agtgacaata ttttgcgcga gctgcgtgtg
    34561 cgggccagcg gcagggccga cgggagtggc acaggtttca ccgaagacgc taacagtttt
    34621 accttttacg ggtgagtaaa actgttgctc gggaatacca aagattgagc gttgttgttg
    34681 gtattcatca aatatgcgcg tcaaaagttc ctcaaacgga atgggacgca taatatcccc
    34741 cataaccctc tccttagctt atacagatat cattgagtga ccggtgttga gaaatccggt
    34801 ggtttagggg gatagagcaa caatcgcacc actcttgcca ggtttggcgg gaaatataaa
    34861 aaaatgtgag gcggctcgaa acttaagggc tttttgtgaa tgaactcaca ggtgccaaat
    34921 gagaattaaa atcactcctg aaaattatta ttgccagccc atttatcaat ttttctcttt
    34981 tctcaaaatc agtgattcct tatcattatg ataatgagtg ccggaaggat taaatatttg
    35041 aacgcaatcg tttaactacg ttttctaaat ttcagtatac ctttccttcg ctgtaataaa
    35101 gtcgtccgga gtatcaatat ctaaaattat ttctgcattt tctatttcga cggaataatg
    35161 atctcccata aggagtgcct gacgcatatt ggtgacattg ggtcgttgga ttgcctgtat
    35221 caagcatggt tttgacacta aaatcggatg gccggggatg ccattatgga gtggcagtat
    35281 tgcgccatca tttcgtaacg accagatttt tctaaaaata tcgatggtga gggttggcat
    35341 gtcaccgtgg gtgagaaaac aatgttctgt ttgcactgcg ggtactgcgg ccttgacgga
    35401 tgttagtaaa ccctgcgcat aatctgggtt gtgaataata gtgatattgc tctggttcgc
    35461 ataacgttcg tgcagttcat taccacgata gccggtgact aaaataattc ggctacaaaa
    35521 ctgcaacgca tttttgatac ttgtatcaag aattgttccc tgttcccagg gtaacatcat
    35581 tttccattgc cccattcttg atgataatcc ggcagcggta attatacagt cgatggctga
    35641 catagtggtt ccattattta taggggctaa taacgcctga taattgcgtt acttcctgat
    35701 ggattaagtt ggggataatt tatcccagag aggtcataaa gactcgtgaa aagtatagtt
    35761 gacccatcgg cattagtcat tgatttaggt gcgcagaaac gtcccactgt gatttctgtt
    35821 gttggtgctg gggggaaaac cagcttgctt ttttggctgg cagaactgct ccaggctagc
    35881 ggcaggcgtg tgttaatcac gacgacgaca catatgttta tgccaacgtc tcactggccc
    35941 gtggttttct gtcgtgatcc cgccatgctt cctcatgcgt ctcttacatc ccccatttca
    36001 ttttgttttc acagctggaa agcgaaccag ggaaaagtgc agggatttac gccagaagcg
    36061 attgatgcac tggtgcaacg accagagtgt gacgtaattc tcattgaagc agatggctcg
    36121 cgtggaatgc cgttaaaagc gcctgatgag cacgaacctt gcatacccaa aagcagttgc
    36181 tgcgtgattg ctgtgatggg aggacatact ttgggcgcga aagtgagcac agaaaatgtc
    36241 catcgctggt cgcagtttgc tgatattacc gggttaacac ctgatgcaac cttgcaactg
    36301 agcgatctcg ttgcgctggt tcgccaccct cagggggcgt ttaaaaacgt accgcaaggt
    36361 tgtcggcgag tctggttcat taaccgtttt tctcaatgtg agaatgcgat tgcacaaagc
    36421 gagctccttc aaccgctgca acaacacgac gtagaggcaa tctggctggg cgatatacaa
    36481 gaacatcctg caatcgcgcg cagatttgtg aattagcggc ccgggtattc cgggttactg
    36541 aaaggatatc cattgaggtt ggtatgaata ttttcacaga ggctgcaaaa ctcgaagagc
    36601 aaaattgtcc gtttgcgatg gcgcaaattg ttgatagccg aggctcgact ccccgccatt
    36661 ctgcacaaat gttagtgcgc gccgatggtt ctatcgtcgg tacaattggt ggcggaatgg
    36721 ttgagcggaa ggtgattgaa gagtcacttc aggcattgca ggaacgtaag ccgcgattat
    36781 tccatggacg tatggctcgt aacggtgcgg atgctgtcgg gtcagattgt ggaggtgcaa
    36841 tgtcagtatt tatcagcgtc catggtatgc gtccacgtct ggtgttgatt ggcgcggggc
    36901 atgtcaaccg ggcgatagcc cagagtgcgg cgctattagg atttgatatt gccgttgccg
    36961 atatttatcg cgaaagcctc aatcctgaac tattccctcc atcaaccacg cttctccatg
    37021 ctgagtcgtt tggtgcagca gtggaagcac tggatattcg ccctgataat tttgtcctga
    37081 ttgccacgaa taatcaggat cgtgaagccc tcgacaaact cattgaacag cccattgcat
    37141 ggttggggtt gctggcaagt cgccgcaagg ttcagctttt cctgcgtcaa ttgcgtgaga
    37201 aaggcgtggc tgaagaacat attgcccgtt tacatgcgcc cgttggttac aacataggtg
    37261 cggaaacgcc gcaggagatc gccatcagcg tgctggcaga aatattacag gtgaaaaata
    37321 acgcgccggg tgggctgatg atgaaacctt ctcatccttc cggacaccag ctggtggtga
    37381 ttcgcggtgc gggagatatc gccagtggtg tggcgctacg tctgtatcat gcgggtttta
    37441 aagtgatcat gctggaagtg gaaaaaccga cagtgattcg ttgtaccgtg gcgtttgccc
    37501 aggccgtgtt cgatggcgaa atgacggtcg aaggcgttac tgctcgcctg gcaaccagct
    37561 ctgcggaagc gatgaaactt accgaacgcg gattcatccc tgtgatggca gatcccgcct
    37621 gttcattgct tgatgaactg aaaccgcttt gcgtggtgga cgctattctg gcgaaacaga
    37681 atttgggaac gcgggcagat atggcaccag taacaatcgc gcttgggccg ggctttactg
    37741 cagggaagga ttgtcatgcg gtaattgaaa caaatcgcgg gcactggctc ggtcaggtga
    37801 tttactctgg ttgtgcgcag gagaataccg gtgttcctgg caatattatg gggcatacca
    37861 cccgacgggt gatccgtgct cctgctgcag gcattatgcg atccaacgtg aaattaggcg
    37921 atctggtgaa agagggcgat gtgattgcct ggattggtga gcatgaaatt aaagcaccgt
    37981 tgacggggat ggtgcgtggc ttgttgaacg acggcctggc agtggtcggt ggttttaaaa
    38041 ttggtgatat cgatcctcgt ggtgaaacgg ctgatttcac cagcgtttct gataaagccc
    38101 gggcgattgg cggcggcgta cttgaggcgt taatgatgtt gatgcatcag ggcgtgaaag
    38161 cgacaaaaga agtgctggaa gtggcttaaa tgaaaatggc cggatggtat tacccacccg
    38221 gccttaaaat tactgacaaa tcaccgttcc ggttttgcct tcaatcccct ctttcgcttt
    38281 gctcaatacg gtaatcagtg cttcacgacc tgcgcgagag cgagcaaacg aggcggcagc
    38341 ttccacttta ggcagcatag aacctttcgc aaaatgacct tcttcgatta agcgttcagc
    38401 atcacttaat gacaggcgat cgagccactg ttcattctct ttaccgaagt taatggccac
    38461 tttttctacc gcagtcagaa tgatcagcat atcggcatca atcatttctg ctaaacgggc
    38521 gctggcccag tctttatcga taaccgcgct ggcaccacgc agatggttac cttcacgaat
    38581 aacaggaata ccaccaccgc caacggtgat caccacctgg cctgcatcta ccagagcttt
    38641 aaccgtttct ttttcaataa tatcaaccgg ttttggcgag gcaacaacac ggcgatagcc
    38701 gcgaccagca tcttctttca gagtgtaacc ctgttttgtc agttgctctg cttcttgctc
    38761 ggtaaagaac gaaccgatcg gcttggtcgg gttgaggaat gccggatcgt tagcgtcgac
    38821 ttcgacctga gtaaccagcg ttgctaccgg tttattaatg ccccgagaaa gcagttcttc
    38881 tcttaaggcg ttttgtagat cgtaaccaat ataaccctgg cttaaggcaa cgcagacaga
    38941 catcggcagc attggcgagt gcgcttcagt ttttgcagca gcttcaaagg cctggttaat
    39001 catgcccacc tgcggcccgt taccatgagt gacgataact tcatgtcctt gggcaattaa
    39061 atcaacaatt gcctgagaag taatttttac cgctttcatt tgtccggcca ggtcatcgcc
    39121 cagcgcattc ccgcccaggg cgagaacaat tttcttactc atttttcctc actcatcatt
    39181 cttgattgtg tatagatccc tgacagtggt tagagcacgg gagggacaaa cggtttgcgt
    39241 cgcaggaaac gacctcgccc ggctttgcct gtaaaggtgc cgtcacagaa aatcgtttca
    39301 ccacgggata aggttctgac aatcgcgccc tgacaggtaa aaccctccca tggcgagtag
    39361 tcggcgttgt cgtggagatg gcgatgctga atttgttggc tctgacgtgg gtcgataatc
    39421 accacgtcgc catcggaacc gggcgctaat aatccttttt gcggccacag gccaaacagc
    39481 ctggcgggca ttgcgctggt taattcaaca aagcgttccg gtgttatacg tcccgtcatc
    39541 acgccactgg aaaacagtaa ctgcatgcgg ttttccacac cgggtaagcc atttgggcag
    39601 cgactgaaat cgcctttaga aatttgcagg cgttgagcca tcgagaaggt gcagtgatcg
    39661 gtcgccacca cgtcaatcgc accatcgctg atgccacacc acagtttgtc ctgctcgcgt
    39721 acgttacgca gcggtgggct aagaatgaac ttcatgccgt cttctgtatc gtaactgcgt
    39781 tcgtccaaca ggagatattg tgggcaggtt tcaacccaga ctggctggtg attcgcacgg
    39841 gcaagacgca gataatccag acctaagccg ttagacaggt gcacgatata gagcggggcg
    39901 ttaccggcaa tttgtgccag gttaatcatg cgggcgatgg cttccgcttc gcattccaga
    39961 gggcgactca aggcatgata gcgcggcgcg gttaaccctg cggcgataaa ttccgcccgc
    40021 ttgctggcga tagccgcatc attttccggg tgcacggtgg tcagcgcgcc ggattcatgc
    40081 agacggcgta atgcctgcaa aacctcgtca tcgttgagtt tgtattgata ggttaaatag
    40141 agtttaaaac tgctcagccc ttcctcgacc atcataggga tttcgtcgag gatggcgtga
    40201 ttaatgtgct ggatcacacc atgaaagctg tagtcgatga ccgccttatg ggcggcatag
    40261 ccacgataaa cctccagttg atggcgtaac cgacacccgt ttgggccaaa tcccatatgg
    40321 tcaataatgg ttgttgtacc gccacacgca gctgcgcggg taccggtaaa aaaatcatca
    40381 caactgcgcg cgatgccgac atcaatattg aaatgcgtat ggacatccac gccgccaggg
    40441 aaaacgtaac agccagtggc atcaatttct tcatacggga gctgcggcga aatattgttg
    40501 cccaactggc gaacaatccc gctttcaatc agcaaatcct gtttggcttg tccatctgcg
    40561 ttaacgacag tgccgttttt gatcaatacg cgcatagcaa actccaaaaa ccctccggcg
    40621 aaccggaggg gaataagtga agagtaatta ttcggttgcc agccagctta acgggattgc
    40681 agcgtacatc gcagcacagg tcaccaggtg agatttccag gttttttcgt taggtgcatg
    40741 cgcttcaggt tctttacccg ggccaaagcc gatcaccgga atgccgtgac ggcccatgat
    40801 agatacgccg ttagttgaga aggtccactt atcaacaacc ggcgctttgc caaacagccc
    40861 ttcgtaggca ttcaccagtg ctttaacggt gaagtgatct tcttccactt tccaggtcgg
    40921 gaagtagcat tcggttgggt aaaccaggcc agtccaggac ggacggtcgt agttgtacat
    40981 agaaacaacc gcgttagctt tctgtactgc aggcagggcg cggatttcgt ccagcgcgcc
    41041 ttcccaggtt tcgccccagg tcagacggcg gtcaatagag acggcgcagc tatctgctac
    41101 agcgcaacgg cttggggatg tgaagaagat ttcagaaacg gtgagggtgc ctttgccgag
    41161 gaattcgtca taacccagac gttgggagag ttcttgtaat tcgccaagaa tcggacccat
    41221 tttgaaaatg gcgttgtcac cgcgttctgg cgcagaaccg tggcagctaa caccctgaac
    41281 atcaatacga atttccatac gaccgcgctg accacggtat atctggcagt cggttggttc
    41341 ggtactgacc acaaattccg ggcgaatgcc ggattgttca ataatgtact gccagcacag
    41401 accgtcgcag tcttcttcct gcacagtacc ggtaaccagc agggtatatt catcttccag
    41461 accgaggtct ttaatgattt taccggcata aaccatagat gccatgccgc cttcctggtc
    41521 ggaagtaccg cgaccaccga ttagctcatc agtttccatg ccttcgtacg gatcgaagtc
    41581 ccagtttttg atgttgccaa tgccgacggt atcgatatga gcgtccattg ccaccagacg
    41641 cgggccgtgg ccgatataac cgagaacgtt gcccatcggg tcgatttcaa ctttatcgaa
    41701 gccgactttt tccatctctt ctttaatacg atgtactacg cgtttttcgt cgcagctttc
    41761 actgggaata gcaaccatgt cgcgcaggaa gcgagtcatg tccgcctggt aatcttttgc
    41821 tttttcaaga atcagtttga atggaatatt cttagccatt atttttctcc agatttttta
    41881 ctccccgttt tgcagggagt agttccaata gggtgattaa ggtgctacag cgtgtttgcc
    41941 ttcccagaca acttcgcggt agtgcttcac gtcggtgtcg ccttcagtgc tgataaccag
    42001 caccacggca tctttgttca gcgccagttt ttccatcagg ctttgacgtt gcgggtgata
    42061 atgaaccgct gcgagaacgc ccaaaccgac agcgccggat tcaccggaga tgatgcgcgg
    42121 gtcgttgccg tacggattac ccagcacgcg catacctaat gcggcaacgc tgtcctggca
    42181 ggagatgaat tgggtggcgc agttacgtag gatttcccag cccagcgggt taggttcgcc
    42241 acaggccagg cccgccatga tggtggccat atcaccgcca acgttgacga tgtcgccttt
    42301 gacgccggag cgataaatac agtcagcttt gtcaggttca acaataatgc tgtgcagatt
    42361 ttgcgggctg tagacgtcga ccagataacc cagcacacca ccggccattg ctccgacacc
    42421 ggcttgcagc agaacgtgcg tcggggttac gcccatttca cgcatttgct cgacggcttc
    42481 atctgccagg gttgcgtagc cttgcatgat ccaggttggg attttggtgt aaccttccca
    42541 tgccgtgtcc tgtaccactt cccagccgtg ctgctgcgcg tgttgcatgg tcaggcgaac
    42601 ggtatcgtca tagttcatat ccgtgacgat gcactcggca ccgaggttca gaatggcgtc
    42661 aacgcgttcc tgagcagaac ctttcggcat gtaaatcacc gcattctgtc cgagttgctg
    42721 tgctgcccac gccacaccgc gcccgtggtt gccgtcggtg gtggtcgcga aagtcatttt
    42781 ttcgccgatg gcatttttca ggtgctcaaa tgacagcgtt tcgatatcaa gatgatattt
    42841 ttcacacaat aattgagcga tggcgtacgc accgccaagc attttgaagg cgttcagacc
    42901 gaatcgtttt gattcgtctt tgacgagaat tttcttcaca ccaaaaaggt ttgcgagatc
    42961 gtccagcgca caaagcggtg ttgggcgata accagctatt ttctggtgga actggcgcgc
    43021 cagtttggcc tggctttgcg aaaagagcgg tgatgtttcg ccgttgaaaa atttgttatc
    43081 ggcgatatca atcttcaatg agaaaacgga catatcctat ccttttggcc tctggaaata
    43141 gagggccgac cagctgatcg gcccgagggg ttatttcacg cgttcttgcg cttctttcag
    43201 cagttgctcg agcagtgcac ctggtttggc gtatttacgg gacaggatca tcgcggcgat
    43261 gatataaggc ttccagctgg cttctttgta ggttgcgata cggtattttt cgaatacgcc
    43321 ttcagtcact tcaccttctt tacaggatac gccgctgata tcagctggca ggcagtgcat
    43381 gtacagggct tcgccatcac gggtcagttc catcatctct tcagtacaat gccagtcttt
    43441 gtgttgcgcg ttctgtgcca gacactgttt ttccagtgct tttaagcctt cgtgatcgtt
    43501 agcgcgcagc aattcggtac gctgttccat cactttgtaa ggtgcccatg acttcggata
    43561 aacgatgtct gcatctttga acgcttcttc catgctggtg acctgacgga agctaccgcc
    43621 agatgcttta gcattgtttt tcgccacttc aaccacatcc gggatcaggt cgtagccttc
    43681 cggatgggcc agggtgacat ccataccgaa gcgagtcatc agaccgatga tgccttgtgg
    43741 tacagagagc ggtttgccat agcttggaga gtaggcccag gtcatggcga tttttttacc
    43801 tttcaggttt tccagtgaac caaagtgttc acgtaaccac gcgaggtcag ccattgactg
    43861 agtcgggtgg tcaatatcgc attgcaggtt cactaaagcc ggacgctgtg gcagtacacc
    43921 ctgcttgtaa ccgtcatcaa gtgcagcgcc aacttcacgc atataggcgt tgcctgcgcc
    43981 cagatacata tcgtcgcgaa taccaatagc gtcggcgcag aaggagatca tattggcggt
    44041 ttcacgcacg gtttcgccgt gagcgatttg tgatttgcct tcgtcgagat cttgttgtgc
    44101 aaggccgagc aggttaagcg cggaagcata agagaagcgg gtacgggtgg agttgtcgcg
    44161 gaatacggaa atacctaatc cactattaaa gactttggtt gagatgtttt cagcacgcag
    44221 tgcttttaat gctgcggcaa cgtccagtac ttgtttcagt tcatctggcg tctgttccca
    44281 cgttaacaaa aaatctttct cgtgaaggtg agaggtcagc gaattgatat ccttaatcag
    44341 ctcattaaca gttttcatca taaagtcccg gtagtggaat gacaggcaaa gtggtttatt
    44401 tcactgtgac ttgccaaagt gattggtgtt ttcacagcta tacctgggac taacaataag
    44461 tgtgccagaa ttatccacgg ggctgcaaaa gactaatttg acggataatg tgtggggttg
    44521 atcacaaatt gatgccagct tccggttgtt tgtaaaatac gaaaacgttt ggcttttttt
    44581 atcgcgttat ttatttgctg ctaagtatca gaaataagat aaagggacag gctatctctc
    44641 ttcagtttga taaactcacc gccgtaattc tcatattgat atagaaatgt gatatctctt
    44701 ccataatttc attagaaaat gataaaaatc ttatgttttt atcaaagcca atttcgcctg
    44761 agttaatatc atcatgatct gtgaaccatc aacgtcttca atgatcgact tttaagtaag
    44821 tctggaaggt aaagctatgg agcttgctac tacgcagtca gtattgatgc aaattcaacc
    44881 gacaattcag cgttttgcca gaatgcttgc cagcgttttg cagcttgagg ttgagatcgt
    44941 tgatgaaaac ttgtgtcgcg tcgccggaac gggcgcgtat gggaagtttc ttggtcgcca
    45001 gttgagcggc aactcacgcc tgctccgcca cgtcctggaa acgaaaactg aaaaagttgt
    45061 gacacagtct cgcttcgatc ccctttgcga aggttgcgat agtaaagaaa attgccgcga
    45121 aaaagcattt ctgggtacgc ctgtcatttt acaggatcgt tgtgttgggg tgataagttt
    45181 gattgccgtt acccacgagc aacaagagca tatcagtgat aatttacgcg aattttctga
    45241 ttatgttcgc catatatcca ccatttttgt ttcgaaactt ctggaggatc aggggccagg
    45301 agataacatc agtaaaatat tcgcgaccat gatcgataat atggatcagg gcgtattagt
    45361 tgttgatgat gaaagtcggg ttcagtttgt taatcagact gccttaaaaa cacttggtgt
    45421 tgtacaaaat aatattattg ggaaacctat ccgtttcaga ccattaacat ttgagagtaa
    45481 ttttactcat ggacatatgc agcatattgt ttcgtgggac gataaaagtg aattaatcat
    45541 tggtcaattg cataacattc agggccgaca attattttta atggcatttc accaatcgca
    45601 taccagtttt tctgtagcaa atgcacctga tgaaccacat attgaacaat tggttggcga
    45661 gtgccgtgtt atgcggcaat taaaacgact cattagccgt attgcaccca gcccatccag
    45721 cgttatggtg gttggtgaaa gcggcacggg taaagaagtc gtcgcccgag caatccataa
    45781 gttgagcgga agacggaata aaccctttat tgctatcaac tgtgccgcga ttccggagca
    45841 gcttctggaa agcgaactgt tcggttatgt taaaggcgca tttactggcg cttctgccaa
    45901 cggtaaaaca gggttgattc aggcggcgaa tacgggcacg ctgtttctcg atgaaatagg
    45961 tgatatgcca ttaatgttgc aggctaaatt actgcgcgct attgaggcgc gtgaaattct
    46021 gccgattggt gccagtagcc caatacaagt cgacattcgc atcatttctg caactaatca
    46081 gaatttggcc cagttcattg ccgaaggtaa attccgcgaa gatctcttct accgacttaa
    46141 tgttatcccg ataactctgc caccgctgcg tgaacgtcag gaagatattg aactattggt
    46201 gcattacttt ttacatctgc atacccgtcg tctgggatcg gtttatcctg gcattgctcc
    46261 cgatgtcgtc gaaatattgc gtaagcatcg ttggcccgga aacctgcgcg agttaagcaa
    46321 tttgatggaa tatctggtta acgtagttcc ttcaggtgaa gttatcgaca gcacgctatt
    46381 gccgccaaat ctgctgaata atggcacaac ggagcaaagt gatgtaacag aggtcagtga
    46441 ggcgcacctg tcactcgatg atgcgggcgg cacggcgctg gaggagatgg aaaagcaaat
    46501 gatccgcgag gcgctttcac gtcataacag caagaagcaa gttgctgatg aactgggcat
    46561 cggcattgct acgctctatc gcaagattaa gaaatatgag ttgttaaaca cataacctca
    46621 gtgaatccgg cctgaattca ggccggatat ccttttactt cgttttctcg caatccagaa
    46681 ctgtatttac aatcatctga taccccgtgc agcgacaaag atttcccgcc agtccgcgac
    46741 gaatttccgt aatggttaat ggcttctcgc gtggtttcgc cagcattgcc gtggtagcca
    46801 taatcaggcc aggcgtacaa aacccgcact gcactgcgcc ggatttcgca taagcctgct
    46861 gaacatgaga aagttttccg cctttcgctt caccttccag cgtgcggatc tcttttcctt
    46921 cagcccaggc ggcaaggtat aagcaactgt ctattgctgt gccgtcgacc aacaccgtac
    46981 aggcaccaca ttcacccacg cagcaccctt gtttgacact tagcagtcct tgttcgcgga
    47041 gtaattccga gagcggcgtg cctggtgcgg cgtgaagctg aaaaggcatc ccgttaatgg
    47101 tgcattcgat ggtaattgtt tcgctgtgat tcattgcaat tttccccccg ccgcggcgac
    47161 ggcttcgcta atcacttttt tggtcatcgt ctggatgaga tgcagacgaa actctttact
    47221 ggcccgccat gaagaacgcg gggcgacatc ttgcaggaca gattcgctga tagcttccag
    47281 cgtttgcagg tttaatggcg cattttgtgc agtctgttcg gcatgttggc agcgaatcgg
    47341 cgttggcgcg gcaacaccaa atgccaggcg taattcgctg aaattgccgt tatccagtcg
    47401 gcaatgtgcg gcgcagccaa tcgttgaaat atccattgcg tcgcgcatgg catatttaaa
    47461 atgcgcgctg cccgcgtgtt ctttcggctg tggcggaaaa tgaaaggcga cgaggatttc
    47521 gtcatgctca agagacactt tgcccggccc ggtgtgaaag ccattaatcg ggacgaaacg
    47581 aacaccgcgc ggggagtgga tctccagttt cgcgtcataa attagcgttg gcgtggcaga
    47641 atctgcgctg gtggcaccgt tgcaaatatt tccaccgtag gtagcgacgt tacggatctg
    47701 cggtccagca atggacgtgg ccgcagcaca taacgccggg agatgacgtt gagttatagg
    47761 atcttctatt agctgggtaa atgtcgttgc agagccgata cgtagcgagc catcttccgc
    47821 cagcgtaatt ccccgcagct ccgccagatt atgaatatca acaatatggc gataacggtc
    47881 attgtgatgg tggagctgaa tcagtacgtc agtgccaccg gcgagcagtt tggcctgcgg
    47941 gttgtcagcc agcaggttga tggcatcggc aagggttgct gcgcgatggt aagaagcaaa
    48001 atcaaacatg atgttatcct caaatcaatc ctgccagatg gaactcttca tataaccgtt
    48061 ttggcgtcag cggcagtgta ttgattgcaa caccggtagc catcttcacc gcgttacgaa
    48121 tagcagcggc aacaggaatt attggtggct cacccagtga cttatgtccg tatgcggatt
    48181 gcggctcatt gatttcgacg aacgcgcttt ccagttgtgg cagatccggc atggtcggca
    48241 ttttgtaatc cagcagattg gggttacgga ccacgccgct tttagcatcg atgatcatct
    48301 cttcaaatag cgcccagcca atgcccattc ccattccgcc gtgtacctga ccttctgcca
    48361 gcagtggatt aagaatatgc cctgaatcat gaacgttgag gatgcggttg atgatgactt
    48421 tgcacagcgc aatatcgacc gtcagatcaa caaaggtaca gccaaacgcc ggtgggttag
    48481 tggtggtttt gatggagctt tcagcagaga gctgcccgcc gcgttcaggg tggtagaaag
    48541 cgtccatcgc caaatctttt aacgacatta acggctcttc cggtcgttca accagcacga
    48601 tatggccttt tatcagggtc agattcatcg ctgactgatg tagcatgact gcggcgtgag
    48661 cgatgatttt ctcttttaat aatagtgccg cactgcgcag cgcaggcgcg gcaacatagc
    48721 tctggcgtga ggcaaatgcg ccgggatcga acggcgtaac gtcggtatct tgagttgaaa
    48781 taacgcgaac gtcgctgacc ggaaccccca cggtttctgc caccatttgc gagaagacgg
    48841 tgtcggcacc ctgaccgatt tccgtcgcgc cgctttgcac gttgatggtt ccatcctgat
    48901 tcatcagaag gcgcgcgcct gctatttcta cgccgacagg ccaggtgtta gaggtgtagc
    48961 taaaacaggc gacgccaacg ccgcggcgca aattgccttg ctggttctgg cattctgcac
    49021 ggcgtttttc ccattcaaag attttccggc ctttttcaag acactccggc aaccctgcgc
    49081 tgtaaatacg tttgcccgtg agcggattag catctccttc gcgggcggcg ttgcgtaaac
    49141 gaatttcaac aggatcaata cctaacgctg tcgcggcgtc atcaagcata gactcaacgg
    49201 caaatacgac ttgtggcgcg ccataaccac gcatcgcacc agccgagggg aggttggtat
    49261 agcaggtctt tgaactgtaa gcgtaggcac aacgaggata aaggtaagcg actttattcc
    49321 cccccgcaga agcgatggag tgcccgtgag atgcataagc gccggtgtta gacagaacat
    49381 ccagactata acctttcaat gttccgtcgc ggttcacgcc catttgcccg tcaatggtaa
    49441 aagcgtggcg ggtacgggtt gcgaggaaac actcttcacg gctaagggaa actttcaccg
    49501 gaatgccgcc aagcttgctg gtcaggaatg ccgccattgg ctcttccagt acatcctgtt
    49561 tattaccaaa accgccaccg acaaacggtt tgatgactcg tacgcatgac cagggaatat
    49621 ccagcgcctg accaaccacg cggcgaacaa tgtgcgggat ctgggtgctg gaaacgatgg
    49681 taattcgcga gtcatcctcc atccacgcca gcgatgttac gctttccatg tgacaatgtt
    49741 gaataacggg ggtctgatag tgcccctgta cctggtagtc ggcggcatcg attgtttgtt
    49801 ggacattacc cgtcgacatc gtgctttgtt tcagtaaatt gccaccgtta tggattggtg
    49861 cagcgtcttc tgccagcgcc gcttctggcg tggtgataac gggtaattct tgccactcaa
    49921 tgctgaccaa ttgcgccgct ttttctgccg tgagttcatc gcgggccacg acgatggcaa
    49981 cggcgtcacc atgatgacga acatggcgag ttagcagtgc gcgatcggcg gtatcgcgct
    50041 tgttttcgtc aagtgtccag gcatgcccag cagtagcaaa tggaatatca ggcacatctt
    50101 cccaggtaaa aatcgccagt acgcccggca aacttctggc ttgttcatca ttaatactta
    50161 cggcataacc atgtgcgata gggctacgta catatttcgc ataacacatg cccgccataa
    50221 cataatcgtc agtatatcgt gcccgcccgg tgaccttagc aatggcatcg acgcgcatgc
    50281 atgattcacc cgtagcggtt gcttcccgcg cttccataga aatcccctcg atttgaacat
    50341 ttggttctta tggcaaagat ttagtctaag caggcaagat ttacgccaga aacgacgata
    50401 agcgttaggg gaggtaggaa gtgtgatgac gttaaaaaaa cacagatatg atacgaaaca
    50461 ggactctgaa taggggcagt gatacgtatc aatatgagtg gcgataaaat caatgtgata
    50521 aattgggatt attacgcatc aggatgggaa ataggttaaa aagtcaggaa gttcgatacc
    50581 tctacagcga atattttgct gatgatactt ccctttgcca aatgaaatta acctctttct
    50641 ctattaccac gtttttccag aagcaagaga ttgggtatca agaggctggc tgctatgata
    50701 aggcgtcttg ttttttaagc gaggaaagat tttgagtgcg ggacgcctga ataaaaaatc
    50761 tctgggtatc gtgatgttgt tatcggttgg actgcttttg gcgggctgtt cgggtagcaa
    50821 atcatccgat acaggaacgt attccggctc cgtttacacc gtgaaacggg gggatacgct
    50881 atatcgtatt tcgcgcacca cgggaaccag cgtaaaagaa ctggcgcgac tgaacggcat
    50941 ttccccccct tacaccattg aagttggtca gaaactaaaa ctgggtgggg cgaaaagtag
    51001 cagtagtaca cgtaaatcaa ccgccaaatc aacgaccaaa accgcatcgg ttacaccgtc
    51061 atcagcggta ccgaaatctt cctggccgcc agtagggcaa cgttgttggt tatggccaac
    51121 gacagggaaa gttatcatgc cgtattcgac agcagatggc ggcaataaag ggattgatat
    51181 ctcagctcca cggggtacac ctatttacgc cgcgggtgca ggaaaggtgg tgtatgtggg
    51241 caaccagctg cgtggctacg gtaatctcat catgattaaa cacagtgaag attacattac
    51301 ggcttacgcc cataatgaca cgatgctggt aaataatggg caaagcgtga aggctgggca
    51361 aaaaatcgcc actatgggga gcacggatgc ggcatctgtt cgcctgcatt tccagattcg
    51421 ttaccgtgca acggcaattg atccgctacg ttacttgccg ccgcagggca gcaagccaaa
    51481 atgctgatgg cgaattaatc agcagtcagc agcgcggcac ttgctaagga gagcgtaagg
    51541 tttataatgc cttacgcatc tcgaagcggg cgtagttcaa tggtagaacg agagcttccc
    51601 aagctctata cgagggttcg attcccttcg cccgctccaa tttatcttca cctgatcaat
    51661 ctgccatttt tgctgtatct gattttctat attatcttaa ttcaatcgct tcagactttt
    51721 cgcaatcgcc gtaatacttc cccattttgt tgtaaataaa aagcgtttac gcaatgcaat
    51781 ccttttgtaa caacccaggt aaaacacctt attttcaagt tgttaaataa gtggttatct
    51841 acgcttttaa caaacgattg atggaatatt ttatgaaagg gaaatctgca ttaactcttc
    51901 tgctcgctgg tatattttcg tgcggcacat gccaggcgac aggggcagaa gtgacgagtg
    51961 aatctgtatt taacattctt aattccacgg gggcagcaac agataaaagt tatttatcgt
    52021 taaatccaga taagtatcct aattatcgtc ttttgattca ctcagccaaa ttacaaaatg
    52081 aaataaagag ccactatacc aaagacgaaa ttcaaggatt gctaacatta acagaaaaca
    52141 cacgaaagtt aacgttgacg gagaaaccct ggggaacgtt tattcttgct tctactttcg
    52201 aagatgacaa aacagcggca gaaacccact atgatgctgt ctggctgagg gatagtttat
    52261 ggggctatat ggcattagta tcagatcagg gaaatagtgt agccgcaaaa aaggttctgc
    52321 tcaccctgtg ggattatatg tctacgcccg accagattaa gcggatgcag gacgtcatta
    52381 gcaaccccaa acgactggat ggagttcccg ggcaaatgaa tgttgtgcat atccgatttg
    52441 acagcaactc ccccgtgatg gcagatgtgc aggaagaagg caagccgcag ctgtggaacc
    52501 ataaacaaaa tgatgcgtta ggtctttatc ttgatctcct tatacaagca atcgacactg
    52561 atacaattaa cgctgaagat tggcaaaaag gtgaccgatt aaaatcggtt gcgctgctga
    52621 ttgcttatct ggataaagcc aatttctatg ttatgaaaga ttccggagca tgggaagaag
    52681 atgcccgatt aaacacatct tccgttgcac tggtgacctc tggactggaa cgtttgtcga
    52741 acttactctc gaagaaagac tctgtttttg tctctgattt actacgcgaa gctaaagcca
    52801 atgaactgga tgaacctctt tcaacaacga ggttgaacca cctcatagat aaaggttatg
    52861 aaagaataac gttacagctt gatttagggg gagaatcccc aggttatctg gaaaaagata
    52921 aacattacag agaagccgat gcggctttac ttaatgttat ttatcctgca aatcttgcaa
    52981 agataaacac cagaagaaaa gagcaggttc taaaaatagt taaaaaacta gcaggtccgt
    53041 atggtataaa aagatacgag aaggataatt atcagtcggc aaatttttgg ttcaatgata
    53101 ttaaaaccga taccgatcaa aattcacatg cgaaaagaga aaagagcttc attccatcaa
    53161 cagaagcaga atggtttttt gactcatggt atgcaaaatc agcagcaatt gtttataaag
    53221 aatcaagaaa ggaagagtat ttgaatgact cagtgcaatt tatgaataga tcattggccc
    53281 aaataacagg cgagaatatg attggtgcaa atggcaggag tgtacctgaa atggcacttc
    53341 ccgaaagcta taactatatc cacaagtcgg gaactttgca tgaagcgcca agccctatca
    53401 ttccgctaaa ttggtcgaaa gcttcaatga ctctcatgtt gaaagagatg tccaacctta
    53461 tcaatgatga gggaattaaa tagttttcat aatgagttac agcacaacgt tgggtattga
    53521 ctgagcaaga tgaacgacaa ggacaacgac atcagcctta tgaggagcaa gaaaatgaaa
    53581 gcaaattcga aaacgatcag aaggatgaaa gttaatgttc ggtctgagga aagtaaatcg
    53641 aaacactcat acgtttgaaa ccacttttca aaattggaaa gaaaatggcg aagatgttgc
    53701 attattaatg ccagaattta gtgctaaatg gttaccgata gcagaagaga gtggcagctg
    53761 gtctggatgg gtattgcttg gagaaatatt tcccttaatt tcatctgagc tcgctgggat
    53821 ggctttaatg ccagaaacgg aaaggttaat aggggaatgg ctcagtttat ccagttcccc
    53881 gttaaatcta aagcatcctg aactaaaata taatcgtttg cacgtaggta aagtatttga
    53941 tggagtattg agccctgctc aaccactaat aagaatatga acaggggaat tgaatctttg
    54001 gttagataaa gtcacagtct gccaatacgg aaacgctcca gtgttagaca aaaaatcatt
    54061 aaattggtcc attcattttg taattggatt tagtaaaaca tgttacagaa gtct
//
LOCUS       DAPHDW010000014        51319 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.014, whole genome shotgun sequence.
ACCESSION   DAPHDW010000014 DAPHDW010000000
VERSION     DAPHDW010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 51319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51319
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.014"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2442
                     /locus_tag="RH057_000805"
     CDS             <1..2442
                     /locus_tag="RH057_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001480735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3127219.1"
                     /translation="SVLIIGCASAYAVEFNKDLIEAEDRENVNLSQFETDGQLPVGKY
                     SLNALINNKRTPIHLDLQWVLIDNQTAVCLTPEQLTLLGFTDEIIEVAQQNLIDGCYP
                     IEKEKQITTYLDKGKMQLSISAPQAWLKYKDANWTPPELWDHGIAGAFLDYNLYASHY
                     APHQGDNSQNISSYGQAGVNLGAWRLRTDYQYDQSFNNGKSQANNLDFPRIYLFRPIP
                     AINAKLTIGQYDTESSIFDSFHFSGVSLKSDENMLPPDLRGYAPQITGVAQTNAKVTV
                     SQNNRIIYQENVPPGPFAITNLFNTLQGQLDVKVEEEDGQVTQWQVASNSIPYLTRKG
                     QIRYTTAMGKPTSVGGDSLQQPFFWTGEFSWGWLNNVSLYGGSVLTNRDYQSLAAGVG
                     FNLNSLGSLSFDVTRSDAQLHNQDKETGYSYRANYSKRFESTGSQLTFAGYRFSDKNF
                     VTMNEYINDTNHYTNYQNEKESYIVTFNQYLESLRLNTYVSLARNTYWDASSNVNYSL
                     SLSRDFDIGPLKNVSTSLTFSRINWEEDNQDQLYLNISIPWGTSRTLSYGMQRNQDNK
                     ISHTASWYDSSDRNNSWSVSASGDNDEFKDMKASLRASYQHNTENGRLYLSGTSQRDS
                     YYSLNASWNGSFTATRHGAAFHDYSGSADSRFMIDADGAEDIPLNNKRAVTNRYGIGV
                     IPSVSSYITTSLSVDTRNLPENVDIENSVITTTLTEGAIGYAKLDTRKGYQIMGVIRL
                     ADGSHPPLGISVKDKTSHKELGLVADGGFVYLNGIQDDSKLTLRWGDKSCFIQPPNSS
                     NLTTGTVILPCISQN"
     gene            2458..3207
                     /locus_tag="RH057_000806"
     CDS             2458..3207
                     /locus_tag="RH057_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3127220.1"
                     /translation="MRYLNTKNLIAAGVLLACMSSIAWGAIIPDRTRIIMNESDKGEA
                     LKLTNQSKNLPYLAQTWIEDTKGNKSRNFIVTVPPMVRLNPSEQIQIRMISQEKIAQL
                     PNDRETLFYFNVREIPPKTDKKNVMQVTMQHALKLFWRPKAIELEDDGVMSYEKVEII
                     RRNDGSIRFNNKMPYHVTLGYIGTNGITMLPQTQSLMVTPYSHADTQFKNVPSAFQVG
                     YINDFGGLSFYEINCPTVNNSCNVSVAKRDK"
     gene            3209..4273
                     /locus_tag="RH057_000807"
     CDS             3209..4273
                     /locus_tag="RH057_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3127221.1"
                     /translation="MRYLLIVIIFIIGFSALPVWAMDCYAEHEGGNTVVIGYVPRISI
                     PSNGKKGDKIWQSSEYFMNVFCNNALPAPSPGEEYPSAWTNIMMFLPGGQDFYNQNSY
                     IFGVTYNGVDYDSTAPNAIAAPECIDIKGAGTFNNHYKNPAVCSGGPEPQLSVTFPAR
                     VQLYIKLAKNANRVNKNLVLPDEYIALEFKGMSGAGAIEVDKNLTFRIRGLNNIHVLD
                     CFVNVALEPADGVVDFGKINSRTIKNTSVSETFSVVMTKDPGAACTEQFNILGSFFTT
                     DILSDYSHLDMGNGLLLKIFHKDGTATEFNRFSQFASFSSSSAPSVTAPFRAELSANP
                     AETVVEGPFSKDVILKITYN"
     gene            complement(4316..4516)
                     /gene="glgS"
                     /locus_tag="RH057_000808"
     CDS             complement(4316..4516)
                     /gene="glgS"
                     /locus_tag="RH057_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface composition regulator GlgS"
                     /protein_id="HDV3127222.1"
                     /translation="MDHSLNSLNNFDFLARSFARMHAEGRPVDILAVTGNMDEEHRTW
                     FCARYAWYCQQMMQARELELEH"
     gene            4785..5414
                     /locus_tag="RH057_000809"
     CDS             4785..5414
                     /locus_tag="RH057_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1449 family protein"
                     /protein_id="HDV3127223.1"
                     /translation="MILFADYNTPYLFAISFVLLIGLLEILALICGHMLSGALDAHLD
                     HYDSITTGHISQALHYLNIGRLPALVVLCLLAGFFGLIGILLQHACIMVWQSPLSNLF
                     VVPVSLLFTIIAVHYTGKIVAPWIPRDHSSAITEEEYIGSMALITGHQATSGNPCEGK
                     LTDQFGQIHYLLLEPEEGKFFTKGDKVLIICRLSATRYLAENNPWPQIL"
     gene            5441..7102
                     /locus_tag="RH057_000810"
     CDS             5441..7102
                     /locus_tag="RH057_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HDV3127224.1"
                     /translation="MDDIVNSVPSWMFTAIIAVCILFIIGIIFARLYRRASAEQAFVR
                     TGLGGQKVVMSGGAIVMPIFHEIIPINMNTLKLEVSRSTIDSLITKDRMRVDVVVAFF
                     VRVKPSVEGIATAAQTLGQRTLSPEDLRMLVEDKFVDALRATAAQMTMHELQDTRENF
                     VQGVQNTVAEDLSKNGLELESVSLTNFNQTSKEHFNPNNAFDAEGLTKLTQETERRRR
                     ERNEVEQDVEVAVREKNRDALSRKLEIEQQEAFMTLEQEQQVKTRTAEQNARIAAFEA
                     ERRREAEQTRILAERQIQETEIEREQAVRSRKVEAEREVRIKEIEQQQVTEIANQTKS
                     IAIAAKSEQQSQAEARANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETK
                     AVELTVRAKAEKEAAEMQAAAIVELAEATRKKGLAEAEAQRALNDAINVLSDEQTSLK
                     FKLALLQALPAVIEKSVEPMKSIDGIKIIQVDGLNRGSAAGDANTGNVGGGNLAEQAL
                     SAALSYRTQAPLIDSLLNEIGVSGGSLAALTSPLTSTTPVAENVE"
     gene            7341..7400
                     /locus_tag="RH057_000811"
     CDS             7341..7400
                     /locus_tag="RH057_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3127225.1"
                     /translation="MMKLVIILIVLLIVSFAAY"
     gene            7717..7776
                     /gene="ibsE"
                     /locus_tag="RH057_000812"
     CDS             7717..7776
                     /gene="ibsE"
                     /locus_tag="RH057_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsE"
                     /protein_id="HDV3127226.1"
                     /translation="MMKFVIILVVLLLLSFPTY"
     gene            complement(7896..9329)
                     /gene="hldE"
                     /locus_tag="RH057_000813"
     CDS             complement(7896..9329)
                     /gene="hldE"
                     /locus_tag="RH057_000813"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HDV3127227.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVSYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(9377..12217)
                     /gene="glnE"
                     /locus_tag="RH057_000814"
     CDS             complement(9377..12217)
                     /gene="glnE"
                     /locus_tag="RH057_000814"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HDV3127228.1"
                     /translation="MKPLSSPLQQYWQTVVERLPEPLAEKSLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQSPQADEWQHYAAWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSHLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPSLQSRSLLPTLSVIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTLIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWVQMVARYGKPNHLNDREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRDRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVPEDCFTAERELVRASWQKWLVEE"
     gene            complement(12240..13541)
                     /gene="ygiF"
                     /locus_tag="RH057_000815"
     CDS             complement(12240..13541)
                     /gene="ygiF"
                     /locus_tag="RH057_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HDV3127229.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRGHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEV
                     WPNGELPADLASRVQPLFSTDFYREKWLVAVDDSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELALAQWQYHEELWVRGNDAAKEQVLAAIGLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTKTAMAKLALTEWLVSKAWQPFLDAKAQSKM
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLTRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            13783..14403
                     /locus_tag="RH057_000816"
     CDS             13783..14403
                     /locus_tag="RH057_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HDV3127230.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPTRKRKDRWMN"
     gene            14467..15705
                     /gene="cca"
                     /locus_tag="RH057_000817"
     CDS             14467..15705
                     /gene="cca"
                     /locus_tag="RH057_000817"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HDV3127231.1"
                     /translation="MKIYLVGGAVRDALLGLPVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYAAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIHTFPMLNPKTIVKLFDSIDAWRKPQR
                     VEQLALTSEADVRGRTGFESADYPQGRWLREAWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(15868..16689)
                     /gene="bacA"
                     /locus_tag="RH057_000818"
     CDS             complement(15868..16689)
                     /gene="bacA"
                     /locus_tag="RH057_000818"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV3127232.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(16780..17148)
                     /gene="folB"
                     /locus_tag="RH057_000819"
     CDS             complement(16780..17148)
                     /gene="folB"
                     /locus_tag="RH057_000819"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HDV3127233.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            17253..17870
                     /gene="plsY"
                     /locus_tag="RH057_000820"
     CDS             17253..17870
                     /gene="plsY"
                     /locus_tag="RH057_000820"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HDV3127234.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPIGWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(17883..18815)
                     /gene="ttdR"
                     /locus_tag="RH057_000821"
                     /gene_synonym="dan"
     CDS             complement(17883..18815)
                     /gene="ttdR"
                     /locus_tag="RH057_000821"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
                     /protein_id="HDV3127235.1"
                     /translation="MLNSWPLAKDLQVLVEIVHSGSFSAAAATLGQTPAFVTKRIQIL
                     ENTLATTLLNRSARGVALTESGQRCYEHALEILTQYQRLVDDVTQIKTRPEGMIRIGC
                     SFGFGRSHIAPAITELMRNYPELQVHFELFDRQIDLVQDNIDLDIRINDEIPDYYIAH
                     LLTKNKRILCAAPEYLQKYPQPQSLQELSRHDCLVTKERDMTHGIWELGNGQEKKSVK
                     VSGHLSSNSGEIVLQWALEGKGIMLRSEWDVLPFLESGKLVRVLPEYAQSANIWAVYR
                     EPLYRSMKLRVCVEFLAAWCQQRLGKPDEGYQVM"
     gene            19022..19933
                     /gene="ttdA"
                     /locus_tag="RH057_000822"
     CDS             19022..19933
                     /gene="ttdA"
                     /locus_tag="RH057_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
                     /protein_id="HDV3127236.1"
                     /translation="MMSESNKQQAVNKLTEIVANFTAMISTRMPDDVVDKLKQLKDAE
                     TSSMGKIIYHTMFDNMQKAIDLNRPACQDTGEIMFFVKVGSRFPLLGELQSILKQAVE
                     EATVKAPLRHNAVEIFDEVNTGKNTGSGVPWVTWDIIPDNDDAEIEVYMAGGGCTLPG
                     RSKVLMPSEGYEGVVKFVFENISTLAVNACPPVLVGVGIATSVETAAVLSRKAILRPI
                     GSRHPNPKAAELELRLEEGLNRLGIGPQGLTGNSSVMGVHIESAARHPSTIGVAVSTG
                     CWAHRRGTLLVHADLTFENLSHTRSAL"
     gene            19930..20535
                     /gene="ttdB"
                     /locus_tag="RH057_000823"
     CDS             19930..20535
                     /gene="ttdB"
                     /locus_tag="RH057_000823"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HDV3127237.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKMVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            20584..22047
                     /gene="ttdT"
                     /locus_tag="RH057_000824"
     CDS             20584..22047
                     /gene="ttdT"
                     /locus_tag="RH057_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HDV3127238.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALIPVPAGLESHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALFLVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVALIVVFYLLRY
                     FFASATAYTSALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(22090..23103)
                     /gene="tsaD"
                     /locus_tag="RH057_000825"
     CDS             complement(22090..23103)
                     /gene="tsaD"
                     /locus_tag="RH057_000825"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HDV3127239.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            23340..23555
                     /gene="rpsU"
                     /locus_tag="RH057_000826"
     CDS             23340..23555
                     /gene="rpsU"
                     /locus_tag="RH057_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HDV3127240.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            23666..25411
                     /gene="dnaG"
                     /locus_tag="RH057_000827"
     CDS             23666..25411
                     /gene="dnaG"
                     /locus_tag="RH057_000827"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HDV3127241.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            25606..27447
                     /gene="rpoD"
                     /locus_tag="RH057_000828"
     CDS             25606..27447
                     /gene="rpoD"
                     /locus_tag="RH057_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HDV3127242.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLMPEDKIRKVLKIAKEPISME
                     TPIGDDEDSHLGDFIEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
     gene            complement(27525..28031)
                     /gene="mug"
                     /locus_tag="RH057_000829"
     CDS             complement(27525..28031)
                     /gene="mug"
                     /locus_tag="RH057_000829"
                     /EC_number="3.2.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
                     /protein_id="HDV3127243.1"
                     /translation="MVEDILAPGLRVVFCGINPGLSSAGTGFPFAHPANRFWKVIYQA
                     GFTDRQLKPQEAQHLLDYRCGVTKLVDRPTVQANEVSKQELHAGGRKLIEKIEDYQPQ
                     ALAILGKQAYEQGFSQRGAQWGKQTLTIGSTQIWVLPNPSGLSRVSLEKLVEAYRELD
                     QALVVRGR"
     gene            28156..28231
                     /locus_tag="RH057_000830"
     tRNA            28156..28231
                     /locus_tag="RH057_000830"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28189..28191,aa:Ile,seq:cat)
     gene            complement(28285..29049)
                     /gene="nfeF"
                     /locus_tag="RH057_000831"
     CDS             complement(28285..29049)
                     /gene="nfeF"
                     /locus_tag="RH057_000831"
                     /EC_number="1.16.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
                     /protein_id="HDV3127244.1"
                     /translation="MNNSPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDG
                     FTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAID
                     FFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLET
                     LSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYF
                     IWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK"
     gene            29326..29949
                     /gene="nfeR"
                     /locus_tag="RH057_000832"
                     /gene_synonym="yqjI"
     CDS             29326..29949
                     /gene="nfeR"
                     /locus_tag="RH057_000832"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HDV3127245.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            complement(30103..31623)
                     /gene="aer"
                     /locus_tag="RH057_000833"
     CDS             complement(30103..31623)
                     /gene="aer"
                     /locus_tag="RH057_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
                     /protein_id="HDV3127246.1"
                     /translation="MSSHPYVTQQNTPLADDTTLMSTTDLQSYITHANDTFVQVSGFT
                     LQELQGQPHNMVRHPDMPKAAFADMWFTLKKGEPWSGIVKNRRKNGDHYWVRANAVPM
                     VREGKISGYMSIRTRATDEEIAAVEPLYKALNAGRTSKRIHKGLVVRKGWLGKLPSLP
                     LRWRARGVMTLMFILLAAMLWFVAAPVVTYFLCVLVVLLASACFEWQIVRPIENVARQ
                     ALKVATGERNSVEHLNRSDELGLTLRAVGQLGLMCRWLINDVSSQVSSVRNGSETLAK
                     GTDELNEHTQQTVDNVQQTVATMNQMAASVKQNSATASAADKLSITASNAAVQGGEAM
                     TTVIKTMDDIADSTQRIGTITSLINDIAFQTNILALNAAVEAARAGEQGKGFAVVAGE
                     VRHLASRSANAANDIRKLIDASADKVQSGSQQVHAAGRTMEDIVAQVKNVTQLIAQIS
                     HSTLEQADGLSSLTRAVDELNLITQKNAELVEESAQVSAMVKHRASRLEDAVTVLH"
     gene            32041..33420
                     /gene="ygjG"
                     /locus_tag="RH057_000834"
     CDS             32041..33420
                     /gene="ygjG"
                     /locus_tag="RH057_000834"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HDV3127247.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGAFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            complement(33462..33794)
                     /locus_tag="RH057_000835"
     CDS             complement(33462..33794)
                     /locus_tag="RH057_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
                     /protein_id="HDV3127248.1"
                     /translation="METVAYADFARLEMRVGKIVEVKRHENADKLYIVQVDVGEKTLQ
                     TVTSLVPYYSEEELMGKTVVVLCNLQKAKMRGETSECMLLCAETDDGSESVLLTPERM
                     MPAGVRVV"
     gene            34013..34996
                     /gene="ebgR"
                     /locus_tag="RH057_000836"
     CDS             34013..34996
                     /gene="ebgR"
                     /locus_tag="RH057_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HDV3127249.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLGIELTN
                     CYEHSGLPDIKNVTGILIVGKPTPALRAAACALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            35180..38272
                     /gene="ebgA"
                     /locus_tag="RH057_000837"
     CDS             35180..38272
                     /gene="ebgA"
                     /locus_tag="RH057_000837"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HDV3127250.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SGQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDNGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGPGLKEYKQVIAPVKIHALDLTRGELKVENKLWFTT
                     LDDYTLHAEVRVEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSR
                     TRYSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKM
                     SGKPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQ
                     SDGEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFT
                     MGINGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRW
                     TALTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLG
                     SNSWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ
                     "
     gene            38269..38718
                     /locus_tag="RH057_000838"
     CDS             38269..38718
                     /locus_tag="RH057_000838"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HDV3127251.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVETDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            38781..40214
                     /locus_tag="RH057_000839"
     CDS             38781..40214
                     /locus_tag="RH057_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3127252.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTIISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDSMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            40348..41418
                     /gene="ygjJ"
                     /locus_tag="RH057_000840"
     CDS             40348..41418
                     /gene="ygjJ"
                     /locus_tag="RH057_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgjJ"
                     /protein_id="HDV3127253.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGVGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNAWVPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWWGDFTPDLGY
                     VIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYDDKYTYPAMM
                     NGYIQYDLAEGITWMNGLEITDGTGQLYLTGLLTPNFAARAWHHTGRADGLDVPGSES
                     GMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIWYEYGGGRFATAFDSR
                     FYMKNASHDPSDQIFLMQYFYW"
     gene            41435..43786
                     /gene="ygjK"
                     /locus_tag="RH057_000841"
     CDS             41435..43786
                     /gene="ygjK"
                     /locus_tag="RH057_000841"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HDV3127254.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVDFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTSQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVKFNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDRVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKTWVAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPATQ
                     YFYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            44112..46130
                     /gene="fadH"
                     /locus_tag="RH057_000842"
     CDS             44112..46130
                     /gene="fadH"
                     /locus_tag="RH057_000842"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HDV3127255.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFAHCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQACLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQESNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(46248..47384)
                     /gene="rlmG"
                     /locus_tag="RH057_000843"
     CDS             complement(46248..47384)
                     /gene="rlmG"
                     /locus_tag="RH057_000843"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417555.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HDV3127256.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     RGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRNGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRMNVETNM
                     PEALDRCEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHHARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKTVKLGRRR"
     gene            47469..47972
                     /locus_tag="RH057_000844"
     CDS             47469..47972
                     /locus_tag="RH057_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HDV3127257.1"
                     /translation="MNNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGGKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            48049..48732
                     /locus_tag="RH057_000845"
     CDS             48049..48732
                     /locus_tag="RH057_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HDV3127258.1"
                     /translation="MQPRLLLRICFSRRTLKIGCLLLLIAGATIFIADRVMVNASKQL
                     TWSDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKNYDE
                     ASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRAIWL
                     AKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMIGPF
                     SEHGCPAKE"
     gene            48811..49797
                     /locus_tag="RH057_000846"
     CDS             48811..49797
                     /locus_tag="RH057_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417558.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3127259.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNALHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKVGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPSFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLASEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKL"
     gene            50080..51045
                     /gene="alx"
                     /locus_tag="RH057_000847"
     CDS             50080..51045
                     /gene="alx"
                     /locus_tag="RH057_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HDV3127260.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
BASE COUNT        12598 a        13533 c        13046 g        12142 t
ORIGIN      
        1 tccgttctca tcattggttg tgcgtcagct tatgccgttg aattcaacaa agatttaatc
       61 gaagccgaag atcgtgaaaa cgttaacctt tcccaatttg aaactgatgg ccaattaccc
      121 gtaggtaaat actcattaaa cgctcttatt aataataaaa ggacgccaat ccatctggac
      181 ctgcaatggg tattaattga taaccaaact gcagtttgtc tgactccaga gcaattaaca
      241 ttattaggct ttactgatga gattattgaa gtggctcagc aaaacctgat cgatggttgt
      301 taccctatcg aaaaagaaaa acaaattaca acttatctcg ataaagggaa aatgcaatta
      361 tccatatctg cacctcaggc atggttaaaa tacaaagatg caaactggac gcctcctgaa
      421 ctttgggatc atggtattgc tggggcattt cttgactaca atttatatgc ctctcattat
      481 gcaccacatc agggcgataa ttcacaaaat ataagttcct atgggcaggc tggggttaat
      541 cttggggcct ggcgcctgcg tactgattac cagtacgatc agtcatttaa caatggcaaa
      601 agccaggcga acaacctgga ttttccgcgt atttatttgt ttcgcccaat cccagcaatt
      661 aatgcaaaac taactatagg tcaatacgat actgaatcct ctattttcga ctctttccat
      721 ttttctggcg tttcgttgaa aagcgatgag aatatgttgc cgcctgatct acgtggttac
      781 gcaccgcaaa tcacgggtgt tgcacaaacg aatgcaaagg tcactgtctc acagaacaac
      841 cgtattattt atcaagaaaa tgttcctcct ggcccatttg ctattactaa tttattcaat
      901 acattacagg ggcaactgga cgtcaaggtt gaagaagagg acgggcaagt cacgcaatgg
      961 caagttgcat ctaatagtat tccttatctg acgcgtaaag ggcagattcg ctacaccact
     1021 gctatgggta aaccgaccag cgttggtggt gattccttac aacaaccctt cttctggact
     1081 ggtgaattct catggggttg gctgaacaat gtatccctgt atggtggttc agttttaaca
     1141 aaccgtgatt atcaatctct ggctgccggc gttggcttta accttaactc attgggttca
     1201 ttatcttttg atgtcacacg atctgatgct cagttgcata atcaggataa agaaacgggt
     1261 tatagctacc gcgctaacta ttcaaaacgt tttgaatcta ccggtagcca gctcactttc
     1321 gctggttacc gtttctctga taaaaacttt gtgacaatga atgaatatat caatgacact
     1381 aaccattaca cgaattatca gaatgaaaaa gagagttata ttgtcacgtt taaccagtat
     1441 cttgaatcat taaggttaaa tacatacgta agtttggctc gtaatactta ctgggacgcc
     1501 agcagtaatg tgaattattc attatcactt agccgcgatt ttgatatcgg gccattaaaa
     1561 aacgtctcca cttctctaac atttagccga ataaactggg aagaagacaa ccaagatcaa
     1621 ctgtacctaa atatttcgat tccctgggga accagtagaa cattgagcta tggtatgcaa
     1681 cgaaatcagg ataacaagat ttcgcatact gcttcgtggt atgactcttc cgatcgaaat
     1741 aattcctgga gcgtttctgc ttcaggcgac aatgatgaat tcaaagatat gaaagcgtca
     1801 ctacgcgcca gttatcagca taataccgag aacggtcgtc tctatctctc cgggacatca
     1861 cagcgagaca gttattattc tctgaatgcc agttggaatg gttcattcac ggcgactcgt
     1921 cacggagccg ctttccatga ctatagtggt agtgctgact cgcgttttat gatcgacgca
     1981 gatggtgcag aagatattcc gttgaacaat aaacgcgcgg taactaatcg gtatggcatc
     2041 ggagttattc catcagtcag cagttacata acaacatcat taagcgttga tacccgaaat
     2101 cttccagaaa atgtggatat cgaaaactcg gttatcacca ccaccttaac cgaaggtgct
     2161 attggctacg ccaaacttga tacccgcaag ggctatcaaa tcatgggggt tattcgcctg
     2221 gcagatggta gtcacccacc actggggatt agcgtaaaag ataaaaccag ccataaagaa
     2281 ttaggactgg ttgctgatgg cggctttgta tacctcaacg gcattcagga tgacagcaaa
     2341 cttactttac gctggggtga taaatcctgt tttattcaac cacccaatag cagcaactta
     2401 accaccggaa cggttatttt accgtgtatt agccaaaatt aattcaggat gaaactaatg
     2461 cgctacttaa acacaaagaa tctcattgca gcaggagtat tactcgcctg catgagcagc
     2521 attgcctggg gtgccatcat cccggaccgt acccgtatca taatgaatga gtcagataaa
     2581 ggggaagcat taaaacttac taatcaaagc aaaaatctac cctatctggc gcaaacatgg
     2641 atagaagata cgaaaggcaa taaatcacgt aactttattg tcacggttcc ccctatggta
     2701 cgtttaaatc ccagtgagca aattcaaata agaatgataa gtcaggaaaa aattgctcaa
     2761 ctgcctaatg acagagaaac gctcttctat tttaacgtgc gagaaatacc gccaaaaaca
     2821 gataaaaaaa atgtgatgca agtcactatg cagcacgcat tgaaactatt ctggcgacca
     2881 aaagctattg aattagaaga tgatggggta atgtcttacg aaaaagttga aatcatccgt
     2941 agaaatgatg gttcgatccg ctttaacaat aagatgcctt atcacgttac gctgggttat
     3001 atcggaacaa acggaatcac aatgttgcca caaacgcaaa gtttaatggt aaccccttat
     3061 agccatgcag atacgcagtt taaaaatgtt ccgtcagctt ttcaggttgg ttatatcaat
     3121 gattttggtg gattaagttt ttatgaaata aactgcccga cggttaataa ctcttgtaat
     3181 gtttccgtag ccaaacgaga taaataattt gcgctactta ttaattgtaa ttatatttat
     3241 tataggtttt agtgcattac ctgtatgggc aatggactgc tatgctgaac atgaaggggg
     3301 aaataccgta gtcattggtt acgtaccaag aatttctatc cccagtaatg gtaaaaaagg
     3361 tgataagatc tggcaaagca gtgagtattt tatgaatgtc ttctgtaata atgcattacc
     3421 tgccccatca cctggagaag aatacccatc agcctggact aatataatga tgtttttacc
     3481 aggcggtcag gacttttata atcagaactc ctatattttt ggcgtaacat acaatggagt
     3541 tgattatgat tcgactgccc ctaatgctat cgcagcacca gaatgcattg atataaaagg
     3601 agcgggtacc tttaataatc actataaaaa ccccgctgtc tgtagtggag gtcctgagcc
     3661 tcaattatca gtaacctttc cagctcgcgt acaactctat attaaattag caaaaaatgc
     3721 caatagagtt aataaaaacc tggtgttacc tgatgaatat atcgctctag agtttaaagg
     3781 tatgagtggc gcaggagcta tagaagtaga caaaaatttg accttcagga ttcgtggatt
     3841 aaataatatt catgtccttg actgctttgt taatgtcgca ctggaaccag ctgatggcgt
     3901 tgttgacttt ggtaaaataa attcccgaac aattaaaaat accagcgtta gtgagacgtt
     3961 tagtgtagtc atgacaaaag atcctggtgc ggcctgtact gagcaattca atattttagg
     4021 gagctttttc actacggata ttttgagtga ttatagccat ctggatatgg gtaatggact
     4081 gctattgaag atatttcata aagatggaac agcaacggaa tttaaccgct tctcacaatt
     4141 tgcttctttt tcatcgtcta gtgcaccttc ggtcaccgca ccattcaggg cagaacttag
     4201 tgcgaatccg gcagagactg ttgttgaggg accgtttagt aaagacgtca tcctcaaaat
     4261 cacctataac tagcatctaa tacaaacact aaaacgggcc atcaggcccg ttatatcagt
     4321 gctctaactc cagctctctt gcctgcatca tctgttgaca ataccaggca taacgtgcgc
     4381 aaaaccaggt tctatgttct tcatccatgt taccagtaac ggcaagaata tcgaccgggc
     4441 gaccttctgc gtgcattctg gcaaaactac gcgccaggaa atcgaaatta tttaaagaat
     4501 taagactatg atccataagc acatcctccg gttatcgcat accttttaag gccatatgct
     4561 ttttctgatt tcagtattcc ccaatcagtg tagtgccttt aaacataacg tgcgtaaata
     4621 tattgtcccc gatcacactt tttagtgaaa aataaaaaac catattaaaa aactttctct
     4681 tccagaactt ctctaacatg tgttattatt aacaaagata aatgtaagca aggaaactat
     4741 cccaaagtaa atattgcgtc actaaatgga cattggagtg acatatgatt ttattcgccg
     4801 actataatac cccttatctt ttcgctattt cctttgtact gttgatcggc cttttagaaa
     4861 ttttagcgct tatctgcggt cacatgctct ccggtgctct tgatgcacat cttgaccatt
     4921 atgattccat aaccacaggt catattagcc aggcacttca ttatctcaat atcggaagat
     4981 tgccagctct cgtcgttctc tgcttgctgg ctggtttttt tggtcttatt ggcatcctgt
     5041 tgcagcatgc ctgcatcatg gtctggcaat caccactgtc gaacctgttc gtggttcctg
     5101 tcagtttact gtttacgatt attgcggtgc attataccgg taaaatagtt gcgccctgga
     5161 ttccacgcga ccatagttca gctattacag aagaagaata tattggtagc atggcattaa
     5221 ttaccggtca tcaagccact tcaggtaatc cctgcgaagg aaaactcacc gatcaatttg
     5281 gccaaataca ttatttacta ctggagcctg aagaaggaaa atttttcact aaaggagaca
     5341 aagtgttaat tatttgccga ctttctgcaa cgcgatattt ggcggaaaac aatccctggc
     5401 cacaaatatt gtaatttatt aatcaaaagg aaatttaata atggatgata ttgttaattc
     5461 tgtgccctcc tggatgttta ccgcgattat tgccgtatgc attctgttta ttattggaat
     5521 tattttcgcc aggctctatc gtcgcgcttc ggcagagcaa gcttttgttc gtactggttt
     5581 aggtgggcaa aaagtggtga tgagcggtgg cgcaatcgtg atgccgatct ttcatgaaat
     5641 aatccccatc aatatgaata ctctgaagct ggaagtcagc cgctcaacca ttgatagcct
     5701 gattacgaaa gatcgtatgc gtgttgatgt agtagtcgct ttctttgtac gggtaaaacc
     5761 gtctgtagaa ggcatcgcca ctgcggcgca gacgttgggg caacgcacgc tatcaccaga
     5821 agacttacgt atgttggttg aagataaatt tgtcgatgcc ctccgtgcaa cagctgcaca
     5881 aatgaccatg catgagttac aggatacccg cgagaacttc gtgcagggag tacaaaatac
     5941 tgtcgcagaa gacctgtcga aaaacggtct tgagctggaa agcgtttcac ttaccaactt
     6001 taaccagacc tcgaaagaac atttcaatcc gaacaatgcc tttgacgccg aaggtttaac
     6061 caaactgact caggagacgg agcgccgtcg tcgcgaacgt aacgaagttg aacaggatgt
     6121 agaagttgcg gtgcgtgaga aaaaccgtga tgcactttcg cgcaagttgg agattgaaca
     6181 acaagaagcg tttatgacgc ttgagcagga gcaacaggtt aaaacccgta ctgccgaaca
     6241 gaatgcacgt attgcggctt ttgaagctga acgtcgtcgt gaagcagagc agacgcgaat
     6301 tctggctgaa cgacaaattc aggaaacgga gatcgagcgc gagcaggccg tccgctcaag
     6361 aaaggttgag gctgaacgtg aagttcgtat taaagagatc gaacaacagc aggtcaccga
     6421 aatcgccaac cagacgaaat cgatcgctat tgccgccaaa tcggaacaac agtcccaggc
     6481 agaagcgcgt gctaaccttg cactcgcaga agcagtaagt gcgcaacaaa acgtagaaac
     6541 cactcgccag accgcagaag ccgatcgtgc taaacaagtt gccctaatcg ctgccgcgca
     6601 ggatgcagaa accaaagcgg ttgaactgac cgtgcgggcg aaagcagaaa aagaagccgc
     6661 agagatgcag gcggcggcta tcgttgagtt agccgaagct acacgtaaaa agggtctggc
     6721 ggaagcagaa gcacaacgtg cgctgaacga tgctatcaac gtactttctg atgaacaaac
     6781 cagccttaaa ttcaaactgg ccttgttgca ggcgctacct gcggtaatag aaaaatccgt
     6841 tgagccgatg aaatctattg acggtatcaa gattattcag gtcgatggtc tgaatcgtgg
     6901 cagcgctgcg ggtgatgcaa acacgggtaa tgtggggggc ggaaacctgg cggaacaagc
     6961 attatcagcc gctctctctt accgcacaca ggcaccgctg attgactcct tgctcaatga
     7021 aattggcgtt tcaggcggct cactggcggc attgacttca cccttaacct caacaactcc
     7081 cgtcgccgaa aacgtagaat aaattttcag ctatgaacgt ccctggcaca aaaagccggg
     7141 gattttttat atctgcgttc cgctaaaaac tgcaaatgat caggtcgttg caacgttttg
     7201 cgtgaccgct cggggaaggc aaaactgcct ctgggaaagc gttgcgcgag gtccggcgct
     7261 catcaacaat cggggagtca gcaaagggct gaaacgggaa agcccctccc gaggaagggg
     7321 ccttacagaa ggaaagggtt atgatgaagc tcgtcatcat actgattgtg ttgttaatcg
     7381 taagtttcgc agcttattaa cagccaatca gaggggggag aaatcctccc tcacccttgt
     7441 tcctttactt taggttgaaa aaaaatcagc gtcaagaagg tttcattaac acatatccct
     7501 ggcccaaaaa gccggggatt ttttatatct gcgttccgct aaaaggtgca aatgctcagg
     7561 acgttgcagc gttttgcgtg accgctcggg gaaggcaaaa ctgcttcagg gaaagcgttg
     7621 cgcggggtcc ggcgctcatc aacaatcggg gagtcagcaa ggggctgaaa cgggaaagcc
     7681 cctcccgagg aaggggcctt atagaaggaa agggttatga tgaagttcgt catcatactg
     7741 gttgtgttgt tactgttaag tttcccgact tactaacaac tgatcagagg ggggagaaat
     7801 cctcccttac ccttgttcct ttactttagg ttgaaaaaac aacagcgtca ataggcctgc
     7861 ctgctacgaa gcgagatctg tgaaccgctt tccggttagc cttttttatc ctgttggatc
     7921 ttcttgatga tgttagtggt cgagcaaccg tcttcgaagt tgagcaccag cacttcgccg
     7981 ccgttggccc agacttcttt actcccggcg atctcttctg gtttatagtc gccgcccttc
     8041 accagtaaat ctggcaggat cccggcgatc aagcgctgcg gcgtgtcttc ttcaaacgac
     8101 accacccagt cgaccgcttc cagtgcgccc agcacaatca tacgctgttc gagcgggtta
     8161 acggggcggg aatccccttt cagccgtttg gtggaggcat cgctgttcac agcgacaatc
     8221 agacggtcac ccagtttgcg agcatttgcc agataggaga cgtgcccggc gtgcaggatg
     8281 tcaaagacac cgttggtcat taccaccttt tcgccgcgtt tacgcgctgc cgctacagca
     8341 agcttcagtt cctcttcggt catcacacca aagccggtat ctgcgcgccc acgcactgca
     8401 ttttccagct cgatcggcga aaccgtggag gttcccagtt tgccgaccac cacgccagcc
     8461 gccgcattgg caaagaagca ggcttcttcc agcgaattac ccgctgccag cgttgccgcc
     8521 aggacgccaa tcaccgtgtc gcccgcaccg gtaacgtcat acacttcctg cgcctgggtt
     8581 ggcatatgca gcggcgcttt acccggttgc agcagcgaca taccctgttc ggaacgggtc
     8641 actaacagag ccgagagttc gtaatcggca atcagtttca tgccgcgctc aacaatctct
     8701 tcttcggtct tacatttacc gacaacagct tcaaattccg agagattcgg cgttaacagc
     8761 gtagcgccgc ggtagcgctc aaaatcggta ccttttggat caatcagcac cggaacgccc
     8821 gctttacgcg ccagttggat catctgctgt acgcttgcca gcgcaccttt ggcgtagtca
     8881 gaaagcacca gcgcgccaat cgaactcagc gcctgattaa tccgctcgtg cagcggctgc
     8941 ggatcaacac cttcgaaacc ttcttcaaaa tccaggcgga tcagctgttg gttgcgggaa
     9001 agtacccgta atttggtgat agtcggatgc gtcggtacag aaacgaagtc gcatttgacg
     9061 ttgacgtcgg ccagagattt actcagcgca cgcgccgcat cgtcaatgcc cgtcagcccg
     9121 accaggcgag cattggcacc gagagaagcg atgttcattg ccacgttagc cgcgccgccc
     9181 ggacgttctt cgatggtatt gactttaacc acgggcaccg gcgcttccgg cgagatacgg
     9241 ctggtgggac cgtaccagta acgatccagc atcacatcac caaccaccat cactcctgca
     9301 cgttcaaact ctggcagcgt tactttcatt cctgtctcct gagagattca aaatttgcgc
     9361 gcgataatac catacttcat tcttccacca gccacttctg ccagcttgcc cgcaccagtt
     9421 cacgctctgc ggtgaagcaa tcctccggca catggcccgg caattcctgt aatgccagat
     9481 gatgaagttc atcgcgcagc gtagtataag cacgggtcag cgccatcgct tcctgctctt
     9541 ccataatgtc gttttgcgcc agtagttcca gaatgcgcac gttgtctgac cagcgcgtta
     9601 acttcggttt ttcatgagcg tagcgcaaca ccagatattg ggtaataaat tcgatatcgg
     9661 taattccgcc ttcatcagct ttgatatcaa agcgatcgcg atgtttattg ccgagatgag
     9721 cgcgcatttt ctcgcgcatt tcccgcactt ccgtttgcag agttttacct tcacgcggca
     9781 gcgtcataat ctcgcgacgc accgcgtcaa agtgcgcggt gagctgcgga tcgccgtaca
     9841 ctacccgcgc acgcaccagc gcctgatgtt cccacgtcca ggcctcgttt ttctgataat
     9901 cggcaaatgc ttctgcggat gtcaccagca ttcccgcggc cccggacggg cgtagccgtg
     9961 catccacttc atacaaaatg ccggaagagg tacgcgtact gaacagatgc ataatgcgtt
    10021 gcgccagacg cagataaaac tgtcgcccgt cgatttcccg ctcgccgtca gtcatcgcat
    10081 ccattgggca atcatggagg aagatcaggt caagatcgga actgtagcct aactcccagc
    10141 cgcccagctt gccgtagccg accaccgcaa aaccacggcc ttcgcgatcg ttgaggtgat
    10201 ttggcttacc gtagcgggcc accatttgaa cccacgcctg ctgaacgacg gcatctatca
    10261 tagcctctgc cagccaggtt aagtgatcgc tcactttcat caccggtagc gtaccagcga
    10321 tatccgctgc ggcgatgcgt aacagctgcg cctgtttgaa ctgacgcagc gcctcaagct
    10381 gttgctcttc gtcatcttcc ggcacgcgca gcaaatactg gcgcaactca tcgcggtagg
    10441 catcggtcgc cgtcggctgg taaagggtgt ttggatcgag caattcatcc agcaataatg
    10501 gataacgcgc cagttggctg gcaatcatcg gcgacgcggc acacagagaa atcaaatgtt
    10561 taagcgccgc ggggaattca ctgagcaatt ctaaataggt ggtgcgggta acaatcccca
    10621 ccagcaaggc ggtaatgcgc gacagcgtaa cggcagcgtc ttcacgcgca cagacatcac
    10681 ttagcagatg cggcatcaga tggtcgagca cctgacgtcc tcgcggcccg atggtgcgct
    10741 tatccagctc tttgcggaaa tcggcaatca gcgttagcac ctgtttgcga tcatcctcgc
    10801 taagatgcgc cagcactggc gtagtgtcat cttcctgcaa cgcatcctgc cacagctcac
    10861 gccactgttc cgacagcgac tcttcctgag tttcactttc gtcgtcgcca atcaattcat
    10921 taaacacccg gcgcacattg gtcatatgtg cggtcagcgc cccggtcagt tgcggccagt
    10981 cagcaaagtc catcgcccac gccagccgcg cacggttaag ctcatcagaa ggaagcgtct
    11041 gggtttgttc gtcgttaatg ctttgcagca ggttttccag acgccgcagg aacagatacg
    11101 ccactcgcaa ttgttcagca tcgttttcag aaagcagatg cagcgcggca atgacgctga
    11161 gcgttggcag taaagagcgc gactgcagcg acggttcgcg cccaccgcga atgagctgga
    11221 acacctgaac gataaattca atttcgcgaa tgccgcctgc gccgagtttg atattgtcgg
    11281 tcaaaccacg tcgacgcact tcacgggcaa tcatcccttt catgttgcgc agcgactgaa
    11341 tcacgctgaa atcgatgtaa cgacggaaaa caaacgggcg cagcatcgca cgcaactcgt
    11401 tagcatagac gccttcgcta tcgcccataa tccgcgcctt gaccatcgcg taacgctccc
    11461 agtcgcgccc ctgctcctgg taataatcct ccaacgcggc aaagctcagc accagcgggc
    11521 cactttcgcc aaacggacgc agccgcatat ccacgcgata gacgaagcca tcctgcgttg
    11581 gttgatccaa cactttaatc agccgctgcc ccatgcgggt aaaaaactgc gcgttatcca
    11641 gttcccggcg tccgccctgc gtacaaccat gttccggcca ggcaaaaatc agatcgatat
    11701 ctgaggagaa attcagttcc ccaccgccca gcttacccat gcctaaaatc agcagcggtt
    11761 gcgcttcgcc ctgcgcattg cacggcgttc cccactcgcg gcagcaagcg tcatacagcc
    11821 agtcacgcgc ggcgacaatc agcgtctccg ccagatggct gagctgctgc aatatgctct
    11881 cttcagtaac caaagccagc gtttgcgccc aggcgatgcg caccataatg cgccgccgga
    11941 atagccgcag ctcgcgcatt aacccggctt cgtcactcac attgctgagc gcctcctgca
    12001 accatgccgc gtaatgctgc cattcgtcgg cctgcggcga ttggctttcc agttccgtca
    12061 gccactctgg atgcgcaatc acgctgtcct gcacaaaatc actgaaagta agtactgact
    12121 tcgcctgtgc gctaagtgat ttctcggcta aaggctctgg cagccgctca acaacggtct
    12181 gccagtactg ctgtaacggt gaagagagcg gcttcataag cgattttatc cttgcctgat
    12241 taacgttttc cgctgtgcaa ccagaacggt tcctgattgt ttgcctcatt acggaaatgt
    12301 tcaatttcaa tgcgttgccc ggtcgcaata gcgtgacgca gcccctgcca gttctccagc
    12361 caggcttgcg cgacgacagg atcatagtaa cccgccagca acagtattga gtcaatatca
    12421 cgcgtcagac gtggcaactg gtcacggtag cgatcgccca acggctggca gaaaacgctt
    12481 ttcagttcag cggcatggcg ggaaagatgg atatcggcaa agcgtttgaa ggaatcgctc
    12541 attttactct gcgctttggc atctaaaaac ggctgccagg ctttgcttac cagccattcg
    12601 gtcaacgcca gcttcgccat tgccgttttg gtagagtaga ccgccgtcac ggcagaaacc
    12661 gcagaagcga tggtcgcttc gcattgagtc agcaaatcac gtaagtgagt gctcgctttg
    12721 cgcggcacaa taccaccgaa cagcatcagt gtatgacgga ccagaccaat ggctgccagc
    12781 acctgttctt tcgccgcatc gttgccgcgt acccacagtt cttcatgata ctgccattgc
    12841 gctaacgcca gctcgagcgc cgcttccagc ccctgttcca catcggcttt tgccgcaaca
    12901 tgcaaaatgg tggtcggttt gatttcacgc gccggattgc cctgcgccag atgatagcca
    12961 cgcgccgctt tgctcaggct gccctggcgt aatccggttt gcgataccag ttggttcgcc
    13021 agtttcagca ccgcgcgcgt gtcgccgcta agcagttcca gttccagctc acagataggt
    13081 tcagcaaatt cccccgcttt cacctccccc tgatcgagag cgatttcaat tcggctgtca
    13141 tcgaccgcca ccagccattt ttcgcgataa aaatcggtgc tgaacagcgg ttgtacgcgg
    13201 gaagcgagat cggcgggcaa ttcgccgttc ggccagactt ccgtcggtaa ctgcgccagg
    13261 tcgagcgtcg gttcgctcaa cgccacgtta tattccgggc gctggtgtaa gccgcctgtc
    13321 actcttcctg caactttcat ggtcatctca tagcgaccgt tttcgccacg aatacgtaag
    13381 cccatatcgt gcccacgcag ccagttatcc ggcgtttcgt agtaaatatt cagcaactgc
    13441 acggggtcat ggtgctcgcc gcccagcgta ttgagatggt cacgcaacgc ctcaacggca
    13501 ctgtgattaa caataaactt taattcgatt tcctgagcca tggcctttac ttatcggtta
    13561 tgtcacataa gtgacgatga acggcgaact taatgcgatc ttttttgtca gtagatagta
    13621 ttttgcgcca aattgccatg caacgggcaa tttgacgggc gtaaaagttt gaagcagtgg
    13681 caagacctga cacagaggat tccgattgat gtcgaatgct ttgcgtagcg acactgatgc
    13741 cactactatc gttccacttt ccatgacaat aacgacagcc tgatgccaaa attacgcctg
    13801 atcggattaa ctttactcgc acttagcgcc actgccgtct cacacgctga agaaacgcgc
    13861 tatgtttctg atgaactgaa tacctgggtc cgtagcggtc cgggagatca ttatcgcctc
    13921 gtgggcacgg ttaacgccgg cgaggaagtg accttattac aaactgacgc caacaccaat
    13981 tatgcccagg tgaaagacag ctctggccgt accgcctgga tcccgttgaa acaacttagc
    14041 actgagccaa gcctgcgctc ccgtgtgcca gatctggaaa atcaggtcaa aaccctgacc
    14101 gataaactca ccaatatcga taacacctgg aatcagcgca ccgcagaaat gcagcaaaaa
    14161 gtggcgcaga gcgacagcgt gatcaacgga ttaaaagaag aaaatcagaa attgaaaaac
    14221 gagctgattg tcgcgcagaa aaaggtcgat gccgccagcg tacagctgga tgacaaacag
    14281 cgcaccatca tcatgcaatg gtttatgtat ggcggtggcg tgctggggct tggcttgctg
    14341 ctcggtctgg tactgccaca cctgatccca acgcgcaaac gcaaagatcg ctggatgaac
    14401 taaatcgcct tcttcgttgc actgtcttat tattaggaat tgtgtatttg acgaagggat
    14461 gatggcgtga agatttatct ggtcggtggt gctgttcggg atgcattgtt agggctaccg
    14521 gtcaaagaca gagattgggt ggtggtcggc agtacgccac aggagatgct cgacgcgggc
    14581 taccagcagg taggccgcga ttttcctgtg tttctgcatc cgcaaacgca tgaagagtat
    14641 gcgctggcac gtaccgaacg gaaatccggt tccggttaca ccggttttac ttgctatgcc
    14701 gcaccggatg tcacgctgga agatgatctt aagcgtcgcg atctgaccat taatgcgctg
    14761 gctcaggacg ataacggcga gattatcgac ccgtacaacg gtctgggcga tctgcaaaat
    14821 cgtctgttgc gccatgtttc ccccgctttt ggcgaagatc cgttacgcgt tctgcgcgtg
    14881 gcgcgttttg ctgcgcgtta tgcccacctc ggttttcgta ttgccgatga aactctgacg
    14941 ctgatgcgcg agatgaccca tgcgggtgaa ctggaacacc tgacgcctga acgggtatgg
    15001 aaagagacgg aaagcgccct caccacccgc aatccccagg tgttcttcca ggtactgcgc
    15061 gattgcggtg cacttcgcgt tttattcccg gaaattgacg ccctgtttgg cgttccggcc
    15121 cctgccaggt ggcatccgga aatcgacacg ggtattcata ccttaatgac gctctcaatg
    15181 gcggcgatgc tgagtccgca ggttgatgtc cgtttcgcaa ctttatgcca cgatctcggt
    15241 aaaggactga cgccgccaga actttggccg cgtcatcatg ggcatggccc ggcgggtgtt
    15301 aagttagtgg aacaactatg ccagcgtctg cgcgtgccaa atgaaattcg cgatttagcc
    15361 agactggtgg ctgagtttca cgatctcatc cacaccttcc caatgctgaa cccaaaaacc
    15421 atcgtcaaat tatttgattc catcgatgcc tggcgtaaac cgcagcgtgt cgagcaactg
    15481 gcgctgacca gcgaggctga cgtacgcggc agaaccggtt ttgaatcagc ggactacccg
    15541 caaggccgtt ggttgcgcga agcctgggaa gtggcgcagt cagtgccgac aaaagcggtc
    15601 gttgaagcgg gatttaaagg tgtggagatt cgcgaggagc tgacccgacg gcggattgcg
    15661 gcggtagcca gttggaagga acaacgttgc ccaaagcctg actgagtata aaactaaacg
    15721 ttaacacgaa ccatcccctc gccccttcgg ggagagggtt agggtgaggg gaacagacca
    15781 gcaccggtgc gaacaatcac ccccaccccg gccctctccc tacagagcga gggagaaaac
    15841 caggcctgct cccgaacctt ttctcaatca aaagaacacg acgtacaccg cagccgccac
    15901 aataaagcga taaatggcga acgggataaa cgaaatgcgc ttaatcaatt gcaggaaggt
    15961 tttaatcgct atcagcgcca ccacaaaagc ggtgataaac ccaacggcaa acatcgggat
    16021 atcgccggtt gtcaggaagc cccagctttt gtagagatcg agcgccgttg cgcccatcat
    16081 catcggtacc gccagcagga acgagaactc ggaagcagcg taacggctca cgcccatcag
    16141 catcccacct gaaatggtgg ccccggaacg ggagaaaccc ggccacagcg ccagacactg
    16201 gaaacagcca atcataaacg cctgacgata ggtcatatca tcaagacccg gcgcacgcgg
    16261 ctctttcggc ttcaggcatt cggcggcaat cagcaacaaa ccgccaacga ccagtgcata
    16321 catcacattt atcgggttaa acaatgactt aatcgtgtcg tggaacaaca gccccagtac
    16381 caccgccgga atcatcccca gcaaaatgtg gatcagcgtt aaacgacctt tgctttctcc
    16441 ttcgtgctgc aacgggcggc caaagtggat gccaatcagg ccaaacagac gccgccagaa
    16501 catcactact accgccagaa ttgatcctaa ctggatcaca acttcaaagg ttttcgccgt
    16561 gtcgccctcg aaccccaaca agtgaccgac aataatcata tggcccgtgc tggataccgg
    16621 cagaaattct gtcaatcctt cgaccacacc caatattgcc gctatcagca gcgagtgcat
    16681 atcgctcatc aataaacccc taaattatta aaatgtaccg cttgtccgga ctactgcagt
    16741 aagaccaggt tataaccgtt tggtttaaca gctgtaaaat taattatttt ctttcagatt
    16801 attgccacgc tcaatgatta cgccaacatt cgccgcccgc gccactgcgc ctggcttgct
    16861 gagtttgata cgcacccacg gcgagttgaa gcgtgctaac agcagctccg ccacctcttc
    16921 agccacgcgt tccaccagcg caaaacgcgc cccctcgacg tggctgacca ccgtttctgc
    16981 aatgtcagcg taactgaggc aatccgccac atcatcactt ttcgccgctt tacggttatc
    17041 ccatgccatt tcgatatcga acactaactt ctgttcgatg gtctgttccc agtcgtaaac
    17101 accaatagtg gtgattaccg aaagttgctc tataaataca atatccatca cgtcctgcct
    17161 gctttttggc taaccggata ccacttccgg cgaaatgtgc gtattatcca cagattcatc
    17221 gttgaacacg aattttcaaa acggaacagc ttatgagtgc aatcgcgcct ggaatgatcc
    17281 tcatcgcgta cctctgcggc tccatttcca gtgccattct ggtttgccgc ttgtgtgggc
    17341 tgcccgatcc gcgaaccagc ggctccggca atccaggcgc aaccaatgtg ttacgtatcg
    17401 gtggcaaggg agcagccgta gcagtactga ttttcgacgt tctgaaagga atgttgcccg
    17461 tctggggcgc gtatgaatta ggtgtcagcc ccttctggct aggcttaatt gccatcgccg
    17521 cctgtcttgg acacatctgg cccgttttct tcggatttaa aggaggaaaa ggcgttgcta
    17581 ccgcttttgg tgccatcgca cccattggct gggatctcac cggagtaatg gcgggaacct
    17641 ggttactgac cgtgctattg agcggatact cgtcgctggg agcgattgtc agtgcactga
    17701 ttgctccatt ttatgtctgg tggtttaagc cacaattcac cttcccggtt tcgatgctct
    17761 cttgcctgat cctgctgcgt catcatgaca acatccagcg tctgtggcgt cgtcaggaga
    17821 caaaaatctg gacgaaattc aaaagaaagc gcgaaaagga tcccgagtga tttctggtgg
    17881 atctacatga cctgatagcc ttcatcgggc ttgcccagcc gttgctggca ccatgccgcc
    17941 agaaattcca cgcagacacg taatttcatg ctgcgataga gcggctcccg gtaaacagcc
    18001 cagatattgg cgctttgtgc atactccggc aatactcgca ccagtttgcc actctccaga
    18061 aacggcaaca catcccactc ggaacgcagc ataatccctt tgccctccag cgcccattgc
    18121 aacactatct cgccgctatt ggaggaaaga tgcccgctaa ccttcaccga ttttttctcc
    18181 tgaccgttcc ccaactccca tattccgtgg gtcatatcgc gctctttggt caccagacag
    18241 tcatgacgac ttaattcctg taatgattgt ggctgcggat atttttgcag atactcaggt
    18301 gctgcgcata atattctttt atttttcgtt aacagatgcg caatataata atcaggaatt
    18361 tcgtcattaa tacgaatatc cagatcaata ttatcctgca ctaaatcaat ttgccgatcg
    18421 aacaattcaa aatgcacctg taactcagga taattgcgca taagttcggt aatggctggc
    18481 gcaatatggc tgcgcccaaa accaaaactg caaccaatac gaatcatccc ctccgggcgc
    18541 gttttgattt gcgtgacgtc atcgaccagt cgctgatatt gggtgaggat ttccagcgca
    18601 tgttcgtaac aacgttggcc actttcagtc agcgccacgc cgcgagcgga gcggttcagc
    18661 agcgttgttg ccagcgtatt ttcaaggatt tggatccgct tggtgacaaa tgcaggcgtc
    18721 tgcccaagcg tcgccgctgc agcactaaaa ctcccgctgt ggacgatttc taccagtacc
    18781 tgcaagtctt tggctaaagg ccagctattc agcatcgtta attatccgca gttgtgataa
    18841 gcgcagtgta tttcgcaaaa cattgccatt ccctgctctg gcgcaatatt cattcttatt
    18901 ttcgcgttga ttcacgaact gttaattaca tattcacggt ggcaaaaaat ataaaaccac
    18961 atttttagtg gtagtttgtg gcggtgaatt tttccagaca aatacaaaaa ctggagttgc
    19021 catgatgagc gaaagtaata agcaacaggc agtgaataag ttgacagaga ttgtcgctaa
    19081 ctttaccgcc atgatttcta cccgaatgcc tgatgacgtg gtggataaac taaaacagct
    19141 aaaggatgcc gaaacgtcgt cgatggggaa aattatctac catacgatgt tcgacaacat
    19201 gcaaaaagcg attgacctga atcgtcctgc ctgtcaggac accggggaga ttatgttctt
    19261 cgttaaagtc ggttcccgct tcccactgct tggcgagctg caaagcatac tcaaacaagc
    19321 cgtggaagag gcaaccgtca aagcgccact acgtcacaat gcggtagaaa tttttgacga
    19381 agtaaacacc ggcaaaaata ccggtagcgg cgtaccgtgg gtcacctggg acatcatccc
    19441 cgacaatgac gatgcggaaa tcgaagttta catggcaggc ggcggctgca cgctacctgg
    19501 ccgctcgaaa gtgttaatgc cgtcagaagg ctacgaaggc gtggtgaaat tcgtcttcga
    19561 aaatatctcc accctcgccg taaacgcctg tccaccggta ctggtgggcg tgggcatcgc
    19621 cacctcggtg gaaaccgccg ccgtactctc gcgtaaagcc attttgcgtc cgattggcag
    19681 ccgccatccc aatccaaaag cggcagagct ggagctacgc ctggaagaag gactcaaccg
    19741 tctggggatt ggtccacaag ggctgaccgg caacagttca gtgatgggcg tgcatatcga
    19801 atctgccgcc cgccatccgt caaccatcgg cgttgctgtc tctaccggct gctgggcgca
    19861 tcgtcgcggc acgctgctgg ttcatgccga tctcaccttt gaaaatctgt ctcacacccg
    19921 gagcgcgtta tgaaaaagat cctgacaacc ccgatcaaag ctgaagatct gcaagatatt
    19981 cgcgtcggcg atgtgatcta cctgaccggt acgctggtga cctgccgcga cgtttgtcac
    20041 cgccgtttga tcgaactgaa acgtccgatc ccttacgatc tcaacggcaa agcgattttc
    20101 cacgctggcc ccatcgtgcg caaaaacggc gacaaatggg agatggtctc cgtcggcccg
    20161 acaaccagta tgcgtatgga aagttttgaa cgtgaattta ttgagcagac cggcgtgaaa
    20221 atggtggtcg gcaaaggcgg aatggggccg ctgaccgaag aaggctgtca gaaattcaag
    20281 gcgctacatg tgattttccc tgcaggctgc gcggtgctgg cggcaaccca ggtggaagag
    20341 attgaagaag tgcactggac agagctcgga atgccggagt cgctgtgggt ctgccgggtc
    20401 aaagagttcg gcccgctgat tgtctctatt gatacccacg gcaacaacct gatagccgaa
    20461 aacaaaaagc tgttcgccga acgccgcgat cccatcgtgg aagagatctg cgagcacgtc
    20521 cattacatca aataaccctc ccggagaggc tcgccccctc tcctttttcg caggcataac
    20581 acgatgaaac cttccactga atggtggcga taccttgcgc cgctggcggt catcgccatt
    20641 attgctctaa ttccggttcc cgcagggctg gagagtcata cctggctcta ctttgccgtt
    20701 tttactggcg tgatcgttgg actgatcctc gaacccgtgc cgggtgccgt ggtggcgatg
    20761 gtgggtatct ccatcatcgc catactctct ccctggctgc tgttcagccc cgaacagctc
    20821 gcccagccag gctttaaatt cactgcaaaa tccctctcgt gggcggtttc cggtttttct
    20881 aattcggtta tctggctgat tttcgccgcc tttatgtttg gcacaggcta tgaaaaaacc
    20941 gggcttggac gccgcattgc gctgtttctg gtgaaaaaga tgggacatcg cacgctgttt
    21001 ctcggctatg cggtgatgtt ctccgagctg atcctcgcac ctgtaacacc gtccaactcg
    21061 gcgcgtggtg cggggattat ctatcccatc atccgtaacc tgccaccgct ctatcaatca
    21121 caaccaaacg acagcagttc gcgcagcatt ggctcgtaca tcatgtggat ggggattgtt
    21181 gccgactgcg tgaccagcgc cattttcttg acggcgatgg caccaaactt gctgttaatt
    21241 ggactgatga aaagcgcatc tcacgccacg ctgagttggg gcgactggtt cctcgggatg
    21301 ttgccgctca gtattttgct ggtgctgctg gttccctggc tggcttatgt gctgtacccg
    21361 ccggtactga agtctggcga tcaggtgccg cgctgggcag agacggaact gcaggcaatg
    21421 ggcccgctct gttcgcgtga aaaacggatg ctggggctga tggtaggcgc gctggtgctg
    21481 tggattttcg gcggtgatta tatcgatgcc gcgatggtcg gttacagcgt ggtggcactg
    21541 atgctgcttc tgcgcattat cagttgggac gacattgtca gtaataaagc ggcgtggaac
    21601 gttttcttct ggctggcctc gcttatcacc ctcgctaccg gactcaacaa caccggtttt
    21661 attagctggt ttggcaaact gttagcaggc agcttaagcg gttattcgcc aacgatggtg
    21721 atggtggcgt tgattgtggt gttttatctg ctgcgctact ttttcgccag cgccacggcg
    21781 tatacctccg ccctcgcacc gatgatgatt gccgctgcgc tggcgatgcc ggaaatcccg
    21841 ctgccggtgt tctgcctgat ggttggcgcg gcaattggtc tggggagcat tcttacacca
    21901 tacgccaccg gacccagtcc gatttactac ggtagtggtt atctgccaac ggcggattac
    21961 tggcgactgg gggcgatttt tgggctgata ttcctcgtat tgctggtgat taccggctta
    22021 ctgtggatgc ccgtggtgtt gctttaagta aggacggtgc cggaaattgc tctccggcac
    22081 ccgatagttt tacgcggccg gtaactccgc cagcggccag cgcggacgca cgctaacgcc
    22141 gagatccgcc gtcgcgcctg ctttaaaccg caccattccg gcataggcga tcatcgcgcc
    22201 gttatcagta caaaactccg gacgcgcgta gaacacttcg ccgcggcgtt ttttcatcat
    22261 ttcagccagc ttcgcccgta acgtacggtt agcactcacg ccgcctgcca tgaccagtcg
    22321 cttaaagcca gtctgatcca acgctcgctt gcacttaatc atcagcgtat cgaccaccgc
    22381 atcttcaaag gcgcgggcga tatcagcacg cgtctggtcg tcggtgccgt tgtcacgaat
    22441 ggtatttgcc gcgaaggttt tcagaccgga gaagctgaaa tccagccccg gacggtcggt
    22501 catcggacgc gggaaaacaa agcgcccggc agtaccctga gccgccattt tcgacagtaa
    22561 cggtccacca ggataatcca gccccagcag cttcgcggtt ttatcaaacg cttcaccggc
    22621 ggcatcatcg atagactcgc cgagcagctc gtactgacca atgccagtca cgctgattaa
    22681 ctgcgtatga ccgccggaca ccagcagcgc gacaaacgga aattccggcg ggttatcttc
    22741 cagcatcggc gctaacagat gcccttccat atggtgtacc gggattgccg gaacgttcca
    22801 ggcaaacgcc agagaacgcc ccacggttgc gccaaccagc agtgcgccga ctaatccagg
    22861 gcctgcggta taggccacag catcaatgtc ttttgccgtt aagccagact cctttagcgc
    22921 cgcctggatc aacggtacgg ttttgcgcac gtgatcgcgg gaggccagtt caggcacgac
    22981 gccgccgtag tcagcgtgca atttcacctg actatacaat tggttggcta acaaaccttt
    23041 ttcatcgtcg taaatggcga tgccggtttc atcgcaggaa gtttcaatac ccagtacacg
    23101 catgacttgt tttacctcgc tttattaccg cgcagtgtag gaccaatgcg ggttgatgta
    23161 aaactttgtt cgcccctgga gaaagcctcg tgtatactcc tcacccttat aaaagtccct
    23221 ttcaaaaagg ccgcggtgct ttacaaagca gcagcaattg cagtaaaatt ccgcaccatt
    23281 ttgaaataag ctggcgttga tgccagcggc aaaccgaatt aatcaaaggt gagaggcaca
    23341 tgccggtaat taaagtacgt gaaaacgagc cgttcgacgt agctctgcgt cgcttcaagc
    23401 gttcctgcga aaaagcaggt gttctggcgg aagttcgtcg tcgtgagttc tatgaaaaac
    23461 cgactaccga acgtaagcgc gctaaagctt ctgcagtgaa acgtcacgcg aagaaactgg
    23521 ctcgcgaaaa cgcacgccgc actcgtctgt actaattccc cgagagcgtt gctctccgat
    23581 cagaccgagt tgtagttgta aggccgtgct tccgaaagga atgcgcggct tattttcgtt
    23641 tatgaattgc taaaaatcgg ggcctatggc tggacgaatc ccacgcgtat tcattaatga
    23701 tctgctggca cgcactgaca tcgtcgatct gatcgatgcc cgtgtgaagc tgaaaaagca
    23761 gggcaagaat ttccacgcgt gttgtccatt ccacaacgag aaaaccccgt cattcaccgt
    23821 taacggtgag aaacagtttt accactgctt tggatgtggc gcgcacggca acgcgatcga
    23881 cttcctgatg aactacgaca agctcgagtt cgttgaaacg gtcgaagagc tggcagcaat
    23941 gcacaatctt gaagtgccat ttgaagcagg cagcggcccc agccagatcg agcgccatca
    24001 gaggcaaacg ctttatcagt tgatggacgg tctgaatacg ttttaccaac aatctttaca
    24061 acaacctgtt gccacgtctg cgcgccagta tctggaaaaa cgcggattaa gccacgaggt
    24121 tatcgctcgc tttgcgattg gttttgcgcc ccccggctgg gacaacgtcc tgaagcggtt
    24181 tggcggcaat ccagaaaatc gccagtcatt gattgatgcg gggatgttgg tcactaacga
    24241 tcagggacgc agttacgatc gtttccgcga gcgggtgatg ttccccattc gcgataaacg
    24301 cggtcgggtg attggttttg gcgggcgcgt gctgggcaac gataccccca aatacctgaa
    24361 ctcgccagaa acagacattt tccataaagg ccgccagctt tacggtcttt atgaagcgca
    24421 gcaggataac gctgaaccca atcgtctgct tgtggtcgaa ggctatatgg acgtggtggc
    24481 gctggcgcaa tacggcatta attacgccgt tgcgtcgtta ggtacgtcaa ccaccgccga
    24541 tcacatacaa ctgttgttcc gcgcgaccaa caatgtcatt tgctgttatg acggcgaccg
    24601 tgcaggccgc gatgccgcat ggcgagcgct ggaaacggcg ctgccttaca tgacagacgg
    24661 ccgtcagcta cgctttatgt ttttgccgga tggcgaagac cctgacacgc tggtacgaaa
    24721 agaaggtaaa gaagcgtttg aagcgcggat ggagcaggcg atgccgcttt ccgcatttct
    24781 gtttaacagc ctgatgccgc aagttgatct gagtacccct gacgggcgcg cacgtttgag
    24841 tacgctggca ctgccattga tatcgcaagt gccgggcgaa acgctgcgaa tatatcttcg
    24901 tcaggaatta ggcaacaaat taggcatact tgatgacagc cagcttgaac gattaatgcc
    24961 aaaagcggca gagagcggcg tttctcgccc tgttccgcag ctaaaacgca cgaccatgcg
    25021 tatacttata gggttgctgg tgcaaaatcc agaattagcg acgttggtcc cgccgcttga
    25081 gaatctggat gaaaataagc tccctggact tggcttattc agagaactgg tcaacacttg
    25141 tctctcccag ccaggtctga ccaccgggca acttttagag cactatcgtg gtacaaataa
    25201 tgctgccacc cttgaaaaac tgtcgatgtg ggacgatata gcagataaga atattgctga
    25261 gcaaaccttc accgactcac tcaaccatat gtttgattcg ctgcttgaac tgcgccagga
    25321 agagttaatc gctcgtgagc gcacgcatgg tttaagcaac gaagaacgcc tggagctctg
    25381 gacattaaac caggagctgg cgaaaaagtg atttaacggc ttaagtgccg aagagcgatc
    25441 gggaagcccc cgacagccgc actgagaggc agcggcaaat atataagtac gccctcgtaa
    25501 ttatcgttgg cggtaaacaa ccgttggatt tcagcgttaa cggctgaagg acatcgggtc
    25561 aatcgcccaa caccaacctc atgaaataag tgtggatacc gtcttatgga gcaaaacccg
    25621 cagtcacagc tgaaacttct tgtcacccgt ggtaaggagc aaggctatct gacctatgcc
    25681 gaggtcaatg accatctgcc ggaagatatc gtcgattcag atcagatcga agacatcatc
    25741 caaatgatca acgacatggg cattcaggtg atggaagaag caccggatgc cgatgatctg
    25801 atgctggctg aaaacaccgc ggacgaagat gctgccgaag ccgccgcgca ggtgctttcc
    25861 agcgtggaat ctgaaatcgg gcgcacgact gacccggtac gcatgtacat gcgtgaaatg
    25921 ggcaccgttg aactgttgac ccgcgaaggc gaaattgaca tcgctaagcg tattgaagac
    25981 gggatcaacc aggttcaatg ctccgttgct gaatatccgg aagcgatcac ctatctgctg
    26041 gaacagtacg atcgtgttga agcagaagaa gcgcgtctgt ccgatctgat caccggcttt
    26101 gttgacccga acgcagaaga agatctggca cctaccgcca ctcacgtcgg ttctgagctt
    26161 tcccaggaag atctggacga tgacgaagat gaagacgaag aagatggcga tgacgacagc
    26221 gccgatgatg acaacagcat cgacccggaa ctggctcgcg aaaaatttgc ggaactacgc
    26281 gctcagtacg ttgtaacgcg tgacaccatc aaagcgaaag gtcgcagtca cgctgccgct
    26341 caggaagaga tcctgaaact gtctgaagta ttcaaacagt tccgcctggt gccgaagcag
    26401 tttgactacc tggtcaacag catgcgcgtc atgatggacc gcgttcgtac gcaagaacgt
    26461 ctgatcatga agctctgcgt tgagcagtgc aaaatgccga agaaaaactt cattaccctg
    26521 tttaccggca acgaaaccag cgatacctgg ttcaacgcgg caattgcgat gaacaagccg
    26581 tggtcggaaa aactgcacga tgtctctgaa gaagtgcatc gcgccctgca gaaactgcag
    26641 cagattgaag aagaaaccgg cctgaccatc gagcaggtta aagatatcaa ccgtcgtatg
    26701 tccatcggtg aagcgaaagc ccgccgtgcg aagaaagaga tggttgaagc gaacttacgt
    26761 ctggttattt ctatcgccaa gaaatacacc aaccgtggct tgcagttcct tgacctgatt
    26821 caggaaggca acatcggtct gatgaaagcg gttgataaat tcgaataccg ccgtggttac
    26881 aagttctcca cctacgcaac ctggtggatc cgtcaggcga tcacccgctc tatcgcggat
    26941 caggcgcgca ccatccgtat tccggtgcat atgattgaga ccatcaacaa gctcaaccgt
    27001 atttctcgcc agatgctgca agagatgggc cgtgaaccga cgccggaaga actggctgaa
    27061 cgtatgctga tgccggaaga caagatccgc aaagtgctga agatcgccaa agagccaatc
    27121 tccatggaaa cgccgatcgg tgatgatgaa gattcgcatc tgggggattt catcgaggat
    27181 accaccctcg agctgccgct ggattctgcg accaccgaaa gcctgcgtgc ggcaacgcac
    27241 gacgtgctgg ctggcctgac cgcgcgtgaa gcaaaagttc tgcgtatgcg tttcggtatc
    27301 gatatgaaca ccgaccacac gctggaagaa gtgggtaaac agttcgacgt tacccgcgaa
    27361 cgtatccgtc agatcgaagc gaaggcgctg cgcaaactgc gtcacccgag ccgttctgaa
    27421 gtgttgcgta gcttcctgga cgattaatcg gtaggccgga tcaggcgtta cgccgcaccc
    27481 ggcactagtc cctctgcaca aacgccacct tttcggtggc gtttttatcg cccacgcact
    27541 accagcgcct ggtccagctc gcgatatgct tcaaccagtt tctctagtga aacgcgactt
    27601 aaaccgctgg gatttggcag cacccaaatc tgcgtcgaac caatggtgag cgtttgtttc
    27661 ccccactgtg caccgcgctg gctgaatccc tgttcatatg cttgtttgcc cagaatcgcc
    27721 aacgcctgcg gctgataatc ttcaattttt tcaatcagct tacgcccacc tgcgtgcagc
    27781 tcctgctttg aaacttcatt ggcttgcacc gttggacggt ctaccagttt ggtgacgcca
    27841 caacgataat ccagcagatg ctgtgcctcc tgcggcttca actgacggtc ggtaaacccg
    27901 gcctgatata tcaccttcca gaagcgattt gctggatgag caaagggaaa accagtcccg
    27961 gcggatgaaa gcccagggtt gataccgcaa aacacgaccc gtaaccctgg agccaaaata
    28021 tcctcaacca tatttactcc tttctaacat caccaggaaa agtataacgg attgaaaata
    28081 cattgtttat aaaaacagca ggcgcacggt aatggctgga ttgcgacacg gagttacttt
    28141 ataatccgct accatggccc cttagctcag tggttagagc aggcgactca taatcgcttg
    28201 gtcgctggtt caagtccagc aggggccacc agatatagca aaggctgacg agaaatcgtc
    28261 agcctttttc tttttatata tcagttactt tgcgtgccag taagccgctg cacgtacccg
    28321 ctgtgggtca tactgttccg cttcaaagcg gcggcttaaa ttcttaacga ctttaccttc
    28381 gccggttatc cagatgaagt aatcatcggc agggatttgc atctgcgcca gacgcgcatc
    28441 taccgcctgc tcatcatgtg ccagccattc gatattaaaa ccatcaaggt gcgcgagata
    28501 atcctgacag gcgttatccc gcacgctaac cagcgcacta acttgcggtt taacggcaag
    28561 tttgctcaac gtttccaggc ggcggcgcag tgcgggcatt ccggattcat cgcagacata
    28621 cagctgatac gcgtaatctt ccggcaccac cagcgaaccg cgcggacctg ccaccgtaag
    28681 tttatcgccc ggttgcgcct gcatcgccca gccgctggcg accccaccgt cgtgaataaa
    28741 gaaatcgatc gccagttcat ggcgtagttc gtcatacagc ggcgtatagt cacgcgacgg
    28801 tgggcgtggt ccttccggcc agacgatgcc ctcttccgtt accgttggcg gcacaaagtg
    28861 agcgtcaggt tgaggaaaga agagtttgct gtgatcgtca aagccgcgcg atgtaaaacc
    28921 gtccagcgcc tcgccgccga ggacaatgcg ctgaaaaccg gcgctgatgc gctcaacgcg
    28981 taacacagtc agttcacgga agcgcagatc attgcgaacg cgctgcgggt agcgggggga
    29041 gttattcatt gttatcgcct tcgtgatagt aatcagatat atctaaataa ttttcgtaaa
    29101 tgataatgat tgttaatcat gataaatgca agcgatttgt agaactgata tatctgaagt
    29161 atgtgaaggc attcagtgcc atagcaatgc tgccggatgc ggcgtgaacg tcttatccgg
    29221 cctggatgtg aggtgcttcc acgtttaaaa atcatttttg tacttgcagt aatgctcaca
    29281 tcagatataa attagatata tctaatcaag caaaaggagg ctgatatgag ccatcatcac
    29341 gaagggtgtt gtaaacatga aggccagcca cgccatgagg gctgctgcaa aggtgagaag
    29401 tcagaacacg agcactgcgg acacggtcac cagcatgaac acggtcaatg ctgcggtggt
    29461 cgccacggtc gcggcggcgg tcgtcggcaa cgtttctttg gtcacggtga attgcgtctg
    29521 gtgattctgg atattctctc gcgcgatgac agccacggtt acgaattgat taaagcgatt
    29581 gagaacctaa cccaggggaa ttacacccca agcccgggcg tcatctaccc aacgctggat
    29641 tttctgcagg agcagtcgct gattaccatc cgcgaagagg aaggtggtaa gaagcagatt
    29701 gcgctgaccg aacaaggcgc gcagtggctg gaagaaaacc gcgaacaggt ggagatgatt
    29761 gaagaacgca tcaaagcgcg ttgcgttggc gcggcgctgc gccagaaccc gcaaatgaag
    29821 cgggcgctgg ataattttaa agcggtgctg gatttacgcg tcaaccagag cgatatcagt
    29881 gatgcacaaa taaaaaagat cattgcggtg atcgaccgcg ccgcttttga tattacgcaa
    29941 ctggattaat cgccgcatcc gccagtggcg cggtgcaatt gccggatgcg acgcttgacg
    30001 cgccttatcc ggcctacacc cgctacacac cccgcaggcc tgataagatg cgccagcatc
    30061 gcatcaggca ttgtgctcca accgccggat ccggcatacc gattaatgca gcaccgtcac
    30121 cgcgtcttcc agtcggctgg cgcggtgttt caccatcgcc gacacctgcg cactctcttc
    30181 caccagctcg gcatttttct gggtgatcag gttaagctca tccactgcac gggtcaggct
    30241 ggaaagccca tcggcctgtt ccagcgttga atggctaatc tgggcgatca actgggtgac
    30301 gtttttcacc tgtgccacaa tatcttccat cgtccgtccg gcggcgtgta cctgctgcga
    30361 accggattgc accttatcag cactggcatc aatcagcttg cgaatatcgt tggcagcatt
    30421 agcgctgcgg ctggctaaat gacgcacttc ccctgccacc actgcaaaac ctttgccctg
    30481 ttcgccggca cgcgccgctt ccaccgccgc attcagggcc agaatattgg tctgaaacgc
    30541 aatatcgtta atcagcgaag taatggtgcc aatgcgctgg gtactgtcgg cgatatcgtc
    30601 cattgtcttg atcaccgtgg tcatcgcctc cccaccctgc actgccgcat tactggcggt
    30661 gatagaaagt ttatcggcag ccgacgccgt ggcagagttc tgtttcaccg acgccgccat
    30721 ttggttcatg gtcgccaccg tttgctgaac gttatcaact gtctgctggg tatgttcgtt
    30781 cagttcatcg gtgcctttcg ccagcgtctc actgccattt ctgacactgg acacctggct
    30841 tgagacatcg ttaattagcc aacggcacat caggccaagc tgccctaccg cgcgtaatgt
    30901 cagccccagc tcatcgctgc gattcaggtg ctcaacacta ttacgctctc cggtcgccac
    30961 cttcagtgcc tgacgggcga cattttctat cgggcgcaca atctgccatt caaaacaagc
    31021 gcttgccaac aataccacta acacacagag gaaatacgtc accaccgggg cagcaacaaa
    31081 ccaaagcatg gccgccagca agataaacat cagggtcatc actccacgcg cccgccagcg
    31141 aagcggtaat gaaggcagtt tacccagcca gcctttacgc accaccaggc ctttatgaat
    31201 acgcttactg gtacgtccgg cgttcagcgc tttgtacagc ggctccaccg ccgcaatctc
    31261 ttcatccgtc gcccgggtac gaatcgacat atagccactg atttttccct cgcgcaccat
    31321 cggtaccgca ttggcccgca cccaataatg gtcgccattt ttgcggcgat ttttcacgat
    31381 gccgctccag ggctcccctt ttttcagggt gaaccacata tccgcaaacg ccgcttttgg
    31441 catatccggg tgacgcacca tgttgtgcgg ctgcccttgt aactcttgca aggtaaagcc
    31501 gctcacctgc acaaaagtgt cattagcatg agtgatatag ctttgcagat cggtagtgga
    31561 catcagagtg gtatcgtccg ccagcggggt attttgctgg gtgacatacg gatgagaaga
    31621 catgttcgcg tcctgtgcag gttatatggt tgttaacttc ttgtcagagt ttatgtcggc
    31681 cccgctgcgg ttatctttaa ccgattaatt tgatttagat cgcaatttgc gattaaagca
    31741 caaatctaat tctttgattt agaatacttt cactctgtta ctatacgaaa acgttaatta
    31801 tcttgcccaa aaatcaggca attattgccc tgaaaacgtg catttgcgca gcaatcatca
    31861 aatccatacc cgacaaaaac cgtgcaaaat aacaacaaat gttaacagat agcattaaat
    31921 attgcacaaa tgataaccga atttgtgttt atcccgattt tcgcgatcgc agccggagtg
    31981 gcgcaatccc tgcaatactt aaatcggtat catgtgatac gcgagcctcc ggagcatatt
    32041 ttgaacaggt taccttcgag cgcatcggct ttagcgtgca gcgcccacgc cctgaatctc
    32101 attgagaagc gaacgctgga tcatgaggag atgaaagcac ttaaccgaga ggtgattgaa
    32161 tacttcaaag agcatgtcaa tccggggttt ttagagtatc gcaaatctgt taccgccggc
    32221 ggggattacg gagccgtaga gtggcaagcg ggaggtttaa atacgcttgt cgacacccag
    32281 ggacaggagt ttatcgactg cctgggaggt tttggaattt tcaacgtggg gcaccgtaat
    32341 ccagttgtgg tttccgccgt acagaatcaa cttgcgaaac aaccgctgca cagccaggag
    32401 ctgctcgatc cgttacgggc gatgttggcg aaaacccttg ctgcgctaac gcccggtaaa
    32461 ctgaaataca gcttcttctg taatagcggc accgagtccg ttgaagcagc gctgaagctg
    32521 gcgaaagctt accagtcacc gcgcggcaag tttactttta ttgccaccag cggcgcgttc
    32581 cacggtaaat cacttggcgc gctgtcggcc acggcgaaat cgaccttccg caaaccgttt
    32641 atgccactgc tgccgggctt ccgtcatgtg ccgtttggca atatcgaagc catgcgcacg
    32701 gctcttaacg agtgcaaaaa aaccggtgat gatgtggctg cggtgatcct cgaaccgatt
    32761 cagggtgaag gtggcgtaat tctgccgccg ccgggctatc tcaccgccgt acgtaagcta
    32821 tgcgatgagt tcggcgcact gatgatcctc gatgaagtac aaacgggcat ggggcgcacg
    32881 ggcaagatgt tcgcctgcga gcatgagaac gtacagccgg atatcctctg ccttgccaaa
    32941 gcgctcggcg gcggcgtgat gccgattggc gcgaccatcg ccactgaaga ggtgttttca
    33001 gttctgttcg acaacccatt cctgcatacc accacctttg gcggcaaccc gctggcctgt
    33061 gcggcggcgc tggcgaccat caatgtgttg ctggagcaga acttaccggc tcaggctgag
    33121 caaaaaggcg atatgttgct ggacggtttc cgtcaactgg cgcgggaata tcccgatctg
    33181 gtacaggaag cgcgtggtaa agggatgttg atggcaattg agtttgttga taacgaaatc
    33241 ggctataact ttgccagcga gatgttccgc cagcgcgtac tggtggccgg aacgctcaat
    33301 aacgccaaaa cgatccgcat tgaaccgcca ctgacactga ccattgaaca gtgtgaactg
    33361 gtgatcaaag cggcgcgtaa ggcgctggcg gccatgcgag taagtgtcga agaagcgtaa
    33421 tatcaaatcg gatggcgata cgacgtcgcc atccgatctt tttatacgac gcgtacgccc
    33481 gcaggcatca tccgctccgg cgttaacaac acgctttcac tgccgtcatc ggtttccgca
    33541 cacaacagca tgcattccga cgtttcacca cgcattttcg ctttttgcag attgcataag
    33601 accaccaccg ttttccccat cagctcttct tcgctgtagt acggcaccag gctggtcacg
    33661 gtttgcagtg ttttttcccc cacatcaacc tgtacgatgt acagcttgtc ggcgttttca
    33721 tggcgtttca cttccacaat ctttccgacg cgcatttcca gacgtgcaaa atcagcgtaa
    33781 gccacggttt ccattgtttc ctcccttcgg gtaaaatttt actaaactat agcaaaagtt
    33841 tttctcaatc ctgtaggcta aaaatggaga atgcaggcgt gatcacattc gtgagccgct
    33901 gtgttaccgt tacagcgtca aagaaacgcg ctttatttac tgaaaacagg tgacccgata
    33961 agcacttcct ctacaatggg ggcgcacatc agggaaagta aaaaaggtaa acatggcaac
    34021 actaaaagac atcgcaatcg aagctggcgt atccctggcg acagtatcca gggtcttaaa
    34081 tgacgatccg acattgaatg tgaaagaaga gacgaaacat cgcattctcg agatcgccga
    34141 aaagctggag tacaagacca gtagtgcccg taaactccag acaggtgcag tcaaccaaca
    34201 ccatattctg gctatctaca gctaccaaca ggagctggag atcaacgatc cttactatct
    34261 ggcgatccgc cacggcattg aaactcagtg cgaaaagctg ggcattgagc tcaccaactg
    34321 ttatgaacac agcggattac cagacattaa aaacgtcacc ggtattttaa ttgtcggcaa
    34381 acccacgccc gccctgcgcg ccgccgcctg tgcgttgacc gacaatatct gttttatcga
    34441 ctttcacgaa cccggcagcg gttacgatgc ggtggatatc gatctggcac gcatcagtaa
    34501 agaaatcatc gacttctata tcaaccaggg cgttaatcgt attggtttta ttggcggtga
    34561 agatgagcct ggcaaggcgg atattcgtga ggtcgccttt gcggaatatg gccgactgaa
    34621 acaagtggta cgcgaagagg atatctggcg cggcggtttt tccagttcgt cgggttatga
    34681 actggcaaaa caaatgctgg cgcgggaaga ctatccgaag gcactgtttg ttgcttccga
    34741 ttccattgct atcggcgtac tgcgggcaat tcatgaacga ggcctgaaca tcccacagga
    34801 tatttcgctt atcagcgtta acgatatccc caccgcgcga tttacctttc cgccgctctc
    34861 caccgtgcgc atccattccg agatgatggg aagtcagggc gttaacctgg tgtatgaaaa
    34921 agcccgcgat ggtcgcgcgc tgccgctgtt agtcttcgtt cccagcaaat taaaactgcg
    34981 cggcacgacc cgttaaatcc ccttacacac tgtccggcaa tcgtttttgc cggacagtgc
    35041 tgccgtttat tttcgtgatc cagttaaagt aaatgcattt acctgctact ttttagtaaa
    35101 aattttacta aactccccag caattacaca aactaccatc accatgaatg gttccgattt
    35161 ctctctaccg ggaggcccta tgaatcgctg ggaaaacatt cagctcaccc acgaaaaccg
    35221 acttgcgccg cgtgcgtact ttttttcata tgattctgtt gcgcaagcgc gtacctttgc
    35281 ccgcgaaacc agcagcctgt ttctgccctt aagcggtcag tggaatttcc acttttttga
    35341 ccatccgctg caagtaccag aagccttcac ctctgagtta atggctgact gggggcatat
    35401 taccgtcccc gccatgtggc aaatggaagg tcacggcaaa ctgcaatata ccgacgaagg
    35461 ttttccgttc cccatcgatg tgccgtttgt ccccagcgat aacccaaccg gtgcctatca
    35521 acgtattttc accctcagcg acggctggca gggtaaacag acgctgatta aatttgacgg
    35581 cgtcgaaacc tattttgaag tctatgttaa cggtcagtat gtgggtttca gcaagggcag
    35641 tcgcctgacc gcagagtttg acatcagcgc gatggttaaa accggcgaca acctgttgtg
    35701 tgtgcgcgtg atgcagtggg cggactctac ctacgtggaa gaccaggata tgtggtggtc
    35761 agcggggatc ttccgcgatg tttatctggt cggaaaacac ctaacgcata ttaacgattt
    35821 cactgtgcgt accgactttg acgaagccta ttgcgatgcc acgctttcct gcgaagtggt
    35881 gctggaaaat ctcgccgcct cccctgtcgt cacgacgctg gaatataccc tgtttgatgg
    35941 cgaacgcgtg gtgcacagca gcgccattga tcatttggca attgaaaaac tgaccagcgc
    36001 cagctttgct tttactgtcg aacagccgca gcaatggtca gcagaatccc cttatcttta
    36061 ccatctggtc atgacgctga aagacgccaa cggcaacgtt ctggaagtgg tgccacaacg
    36121 cgttggcttc cgtgatatca aagtgcgcga cggtctgttc tggatcaata accgttatgt
    36181 gatgctgcac ggcgtcaacc gtcacgacaa cgatcatcgc aaaggccgcg ccgttggaat
    36241 ggatcgcgtc gagaaagatc tccagttgat gaagcagcac aatatcaact ccgtgcgtac
    36301 cgctcactac ccgaacgatc cgcgttttta cgaactgtgt gatatctacg gcttgtttgt
    36361 gatggcggaa accgacgtcg aatcgcacgg ctttgctaat gtcggcgata tcagccgtat
    36421 taccgacgat ccgcagtggg aaaaagtcta cgtcgagcgc attgttcgcc atattcacgc
    36481 gcagaaaaac catccgtcga tcatcatctg gtcgctgggc aatgaatccg gctatggctg
    36541 taacatccgc gcgatgtacc acgcagcgaa ggcgctggat gacacgcgac tggtgcatta
    36601 cgaagaagat cgcgatgctg aagtggtcga tattatttcc accatgtaca cccgcgtgcc
    36661 gctgatgaat gagtttggtg aatacccgca tccgaagccg cgcatcatct gtgaatatgc
    36721 tcatgcgatg gggaacggac caggcgggct gacggagtac cagaacgtct tctataagca
    36781 cgattgtatt cagggacatt atgtttggga gtggtgcgac cacgggatcc aggcgcagga
    36841 tgacaacggc aacgtctggt ataaattcgg cggcgactac ggcgactatc ccaacaacta
    36901 taacttctgt cttgatggtt tgatctattc cgatcagacg ccgggaccgg gcctgaaaga
    36961 gtacaaacag gttatcgcgc cggtaaaaat ccacgcgctg gatctgactc gcggcgagct
    37021 gaaagtcgaa aataaactgt ggtttaccac gcttgatgac tacaccctgc acgcagaggt
    37081 gcgcgtcgaa ggtgaaacgc tcgcgacgca gcagattaaa ctgcgcgacg ttgcgccgaa
    37141 cagcgaagcc cccttgcaga tcacgctgcc gcagctggac gcccgcgaag cgttcctcaa
    37201 cattacggtg accaaagatt cccgcacccg ctacagcgaa gccgggcatt ctatcgccac
    37261 ttatcagttc ccgctgaagg aaaacaccgc gcaaccagtg cctttcgcac caaataatgc
    37321 gcgtccgctg acgctggaag acgatcgttt gagctgcacc gttcgcggct acaacttcgc
    37381 gatcaccttc tcaaaaatga gtggcaaacc gacatcctgg caggtgaatg gcgagtcgct
    37441 gctgacccgc gagccaaaga tcaacttctt caagccaatg atcgacaacc acaagcagga
    37501 gtacgaaggg ctgtggcaac cgaatcattt gcagatcatg caggaacatc tgcgcgactt
    37561 tgccgtagaa cagagcgatg gtgaagtgtt gatcatcagc cgcacagtta ttgccccgcc
    37621 ggtgtttgac ttcgggatgc gctgcaccta catctggcgc atcgctgccg atggccaggt
    37681 taacgtggcg ctttccggcg agcgttacgg cgactatccg cacatcattc cgtgcatcgg
    37741 tttcaccatg gggattaacg gcgaatacga tcaggtggcg tattacggtc gtggaccggg
    37801 cgaaaactac gccgacagcc agcaggctaa catcatcgat atctggcgca gcaccgtcga
    37861 tgccatgttc gagaactatc ccttcccgca gaacaacggc aaccgtcagc atgtccgctg
    37921 gacggcactg actaaccgcc acggcaacgg tttgctggtg gttccgcagc gcccaattaa
    37981 cttcagcgcc tggcactata cccaggaaaa catccacgct gcccagcact gtaacgagct
    38041 gcagcgcagt gatgacatca ccctgaacct cgatcaccag ctgcttggcc tcggctccaa
    38101 ctcctggggc agcgaggtgc tggactcctg gcgcgtctgg ttccgtgact tcagctacgg
    38161 ctttacgttg ctgccggttt ctggcggaga agctaccgcg caaagcctgg cgtcgtatga
    38221 gttcggcgca gggttctttt ccacgaattt gcacagcgag aataagcaat gaggatcatc
    38281 gataacttag aacagttccg ccagatttac gcctctggca agaagtggca acgctgcgtt
    38341 gaagcgattg aaaatatcga caacattcag cctggcgtcg cccactccat cggtgactca
    38401 ttgacttacc gcgtggagac agactccgcg accgatgcgc tatttaccgg gcatcgacgc
    38461 tattttgaag tgcattacta cctgcaaggg cagcaaaaaa ttgaatatgc gccgaaagag
    38521 acattacagg tagtggaata ttatcgtgat gaaactgacc gtgaatattt aaaaggctgc
    38581 ggagaaaccg ttgaggtcca cgaagggcaa atcgttattt gcgatatcca tgaagcgtat
    38641 cggtttatct gcaataacgc ggtcaaaaaa gtggttctca aagtcaccat cgaagatggt
    38701 tatttccata acaaataaca actacggcgg caaaaggagt ttgccgccac cgctacccta
    38761 cacattttcg gagatgtgtt atgtctgata ccaaacgtaa tacaatcggc aaattcggct
    38821 tgctctcgct gacttttgcc gccgtttaca gctttaacaa cgttatcaat aataatattg
    38881 agcttggact ggcctcggca ccgatgtttt tcctcgcgac gattttttat tttattccct
    38941 tctgtctgat catcgcagaa tttgtttcgt taaataaaaa ctcagaagcc ggtgtctacg
    39001 cgtgggtaaa aagttcgctg ggcggacgtt gggcatttat tactgcctat acctactggt
    39061 tcgtaaacct gttctttttc acctcactgt tgccgcgcgt tattgcttat gcttcgtatg
    39121 ccttcctcgg ctacgaatat attatgacgc cggttgccac caccattatc agtatggtgc
    39181 tgttcgcctt ctccacctgg gtttccacca acggggcgaa aatgttgggg ccaattacct
    39241 ccgtcacttc aacgctgatg ctgctgttaa cgctctccta cattttactg gcaggtacgg
    39301 cgctggttgg cggcgtacag cctgctgacc ccatcaccgt tgactcgatg atcccgaact
    39361 tcaactgggc gttcctcggc gttaccacct ggatctttat ggccgcaggt ggcgcggagt
    39421 ccgtcgctgt gtacgttaac gacgtcaaag gcggttcgaa atcgttcgtt aaagtgatca
    39481 tcctcgccgg gatttttatc ggcgtactgt attccgtctc ctcggtgctg attaacgtct
    39541 tcgtcagcag caaagagttg aaatttaccg gcggatcggt gcaggtattc cacggcatgg
    39601 cggcgtattt tggtctaccg gaagcgttga tgaatcgctt tgtcggtctg gtgtccttta
    39661 ccgcgatgtt cggttccctg ctgatgtgga ccgcaacgcc ggtgaaaatt ttcttctccg
    39721 aaatcccgga aggcatcttt ggtaagaaaa ccgtcgaact gaacgaaaac ggcgttccgg
    39781 cgcgcgcagc gtggatccag ttcctgatcg tcatcccgct gatgattatc ccgatgctcg
    39841 gttccaatac cgtgcaggat ctgatgaata ctattattaa tatgaccgcc gcagcgtcca
    39901 tgcttccgcc gttattcatc atgctggctt acctgaattt acgcgccaaa ttagatcacc
    39961 tgccacgcga tttccgtatg ggctcccgcc gcaccggtat tatcgttgtt tcaatgctga
    40021 ttgcgatatt tgccgtaggg tttgtcgctt cgacattccc gactggcgcg aatattctga
    40081 ccatcatttt ttataacgtc ggcggtattg ttatcttcct cggctttgcg tggtggaaat
    40141 acagtaaata tataaaggga ttaacggctg aagagcgcca tattgaagcg acgccagcca
    40201 gcaatgttga ttaagcagaa taataaaaaa tgagcaggca ttttccctct tcctggatga
    40261 ggagagggaa aatagtttct gcctttatat ttttataatt acaacgataa aaggctgtac
    40321 tttttcttta gctcatggat taacacaatg aaattaatca ctgcaccatg cagagcatta
    40381 cttgctctgc cgttttgcta cgccttttct gcggcaggag aagaagcacg tccggcagaa
    40441 catgacgaca caaaaacacc tgcaattacc tcgacatctt ctccttcatt tcgtttttac
    40501 ggcgaattag gggttggtgg atatatggat ttagagggtg agaataaaca taaatacagc
    40561 gacggtacct atattgaagg tggcctggag atgaagtacg gctcctggtt cggcctgatt
    40621 tacggcgaag gctggaccgt gcaggccgac cacgacggca atgcctgggt gccagaccat
    40681 agctggggtg gtttcgaggg cggaattaac cgtttctatg gcggttatcg taccaatgat
    40741 ggcaccgaaa tcatgctcag tctgcgtcag gattcctcgc tggatgacct gcaatggtgg
    40801 ggcgacttca cccccgatct gggctacgtc attcccaata cccgcgacat tatgaccgcg
    40861 ctgaaggtgc agaacttaag cggcaacttt cgttatagcg tcaccgccac acctgccgga
    40921 catcatgacg aaagcaaagc ctggctacat tttggcaagt acgatcgcta tgacgacaaa
    40981 tacacctatc cggcaatgat gaacggttac atccagtatg accttgccga aggcatcacc
    41041 tggatgaacg gtctggaaat caccgacggc acaggacagc tctatctcac gggcctgcta
    41101 actcctaact ttgccgctcg cgcctggcac cataccggac gcgccgacgg gctggacgta
    41161 ccgggaagtg aaagtgggat gatggtgagc gccatgtatg aagcgttaaa gggcgtttat
    41221 ctctccaccg cttataccta cgccaaacat cgccctgacc acgctgacga tgaaaccacc
    41281 tctttcatgc agtttggtat ctggtacgaa tacggcggcg gacgtttcgc cacggctttt
    41341 gatagccgct tctacatgaa aaatgcctct cacgatccca gcgaccaaat cttcctgatg
    41401 caatatttct actggtaata aggactgtaa tttcatgaaa ataaaaacta ttttaacgcc
    41461 agtaacctgc gctctgctga taagtttttc cgcccatgcc gctaacgccg acaattataa
    41521 aaacgtgatt aaccgtactg gcgcgccgca gtacatgaag gattacgatt acgacgatca
    41581 ccagcgtttt aatccgtttt tcgatctcgg agcctggcat ggtcatctgt tgccagacgg
    41641 ccctaacacc atgggcggct ttccgggcgt tgcgctgctg acggaagagt acatcaactt
    41701 tatggccagc aatttcgacc gcctgaccgt ctggcaggac ggcaagaaag tcgactttac
    41761 gctggaggca tacagtattc ccggtgcgct ggtgcaaaaa ctgacagcaa aagatgtgca
    41821 agtcgaaatg actctgcgct tcgccacgcc gcgcacgtca ctactggaaa ctaaaatcat
    41881 cagcaataaa ccgctggatc tggtgtggga tggcgaactg ctggaaaaac tggaagcgaa
    41941 agaagggaaa ccgctttccg ataaaaccat tgctggcgaa taccctgact accagcgcaa
    42001 aatcagcgcc acccgtgatg gcctgaaagt cacctttggc aaagtgcgcg ccacctggga
    42061 tctgctgacc tccggtgaat cagaatatca ggtgcataaa tccctgccgg tgcagactga
    42121 aatcaacggc aatcgcttta ccagtaaggc acatatcaac ggttcgacca cgctctatac
    42181 cacctattcc catctgctga cctctcagga agttagcaaa gagcaaatgc agatccgcga
    42241 tattctggcg cgtccggcgt tttatctcac cgcctcgcag caacgctggg aagagtatct
    42301 gaagaaaggg ttaaccaatc cggatgcgac gccggaacag acgcgcgtcg cggtgaaagc
    42361 gatcgaaacg ctcaacggta actggcgttc gcctggcggg gcggtgaaat ttaacaccgt
    42421 cacaccgtcg gtgaccgggc gctggttctc cggcaatcag acctggccgt gggatacctg
    42481 gaagcaggcg tttgcgatgg cgcatttcaa tccggacatc gccaaagaga atatccgcgc
    42541 ggtcttctcc tggcagatcc agccaggcga ccgcgtgcgt ccgcaggatg tgggctttgt
    42601 ccccgacctg atagcgtgga accttagccc cgagcgtggt ggcgatggcg gcaactggaa
    42661 cgaacgtaat accaaaccca gccttgccgc ctggtcggtg atggaagtgt ataacgtcac
    42721 ccaagataaa acctgggtgg cagagatgta cccgaaactg gtggcctatc acgactggtg
    42781 gttacgtaac cgcgatcaca acggcaacgg cgtgccggaa tatggcgcga cccgcgacaa
    42841 agcccacaac actgagagcg gcgagatgct gtttacggtg aaaaaaggcg ataaagaaga
    42901 gacgcagtct gggctgaaca actacgcccg cgtggtggag aaaggccagt acgacagtct
    42961 ggaaattccg gcacaggttg ctgcatcgtg ggaatcgggg cgtgatgatg ccgccgtctt
    43021 tggttttatc gacaaagaac agttggataa atatgtcgct aatggcggca aacgtagcga
    43081 ctggacggtg aaattcgccg aaaaccgcag tcaggacgga acgttgctgg gctactcgct
    43141 attgcaggag tcggtggatc aggccagcta tatgtacagc gataaccatt atctggcgga
    43201 gatggcgacg attctcggta aaccggaaga agctaagcgc tatcgccagt tggcacagca
    43261 gctcgcggac tacatcaaca cctgtatgtt cgacccggct acacagtact tctatgacgt
    43321 gcgtattgaa gataaaccgc tggcgaacgg ctgcgcgggc aaaccgattg ttgagcgcgg
    43381 taaagggccg gaaggctggt cgccgctgtt taacggtgcg gcaacgcagg ccaatgccga
    43441 cgcggtggtg aaggtgatgc tcgatcctaa agagttcaac acctttgtcc cgctgggaac
    43501 ggcggcgtta accaatccag cttttggcgc tgatatctac tggcgcgggc gcgtatgggt
    43561 ggatcagttc tggtttggtc tgaaagggat ggagcgttac ggttatcgtg atgatgccct
    43621 gaagctggcg gatacgttct tccggcacgc caaaggatta accgccgatg gcccaattca
    43681 ggagaattac aacccgctga ctggcgcgca gcaaggcgca ccaaatttct cctggagtgc
    43741 cgcgcatttg tatatgttgt ataacgattt tttccgtaag caataatttg caactgctgg
    43801 cggatgcggc gtaaacgcct tatccgccct acatgtgcaa tcctcctggc cggataaaac
    43861 gcgacaagcg tcgcatccgg catctgcacc gtgccactgg cggatgcggc gtgaacgcct
    43921 tatccgccct acatgtgcaa tcccccaggc cggataaaac gcggcaagcg tcgcatccgg
    43981 cgttaccacc gggcgtattc tttttgaatc ccatcacaaa ccccgcactc cccttttccc
    44041 ttttctccgg cgacggctaa attagaactc atccgaccac ataacaatta ttttacatac
    44101 tggacaattt tatgagctac ccgtcgctgt tcgccccgct ggatttaggt tttaccacgt
    44161 taaaaaaccg cgtgttgatg ggctcaatgc acaccgggct ggaggaatac ccggacggtg
    44221 ccgagcggct ggcagcgttt tatgccgaac gcgcccgtca cggcgtggcg ctgattgtca
    44281 gcggcggcat tgcaccagat ttaacaggcg ttggcatgga aggtggcgca atgctcaacg
    44341 acgccagcca gatcccacac catcgcacca ttaccgaagc ggtacatcag gaaggcggca
    44401 aaatagccct gcaaattttg cataccgggc gctacagcta ccaaccgcat ctggtcgctc
    44461 cctccgcatt gcaggccccc atcaaccgtt tcgttcctca cgaactcacg catgaagaga
    44521 tcctgcaact gatcgacgat ttcgcccact gcgcgcaact ggcacgggag gcaggatacg
    44581 acggtgtaga ggtaatgggg tccgaagggt atttgatcaa cgaatttctg acgctgcgca
    44641 ccaatcagcg tagtgaccag tggggcggcg attaccgcaa ccggatgcga tttgccgtag
    44701 aagtagtgcg tacggtgcgc gaacgcgtcg gcaacgactt cattattatc taccggttgt
    44761 cgatgctcga cctggtcgaa ggcggcggca cttttgccga aacggtagaa ctggcgcagg
    44821 ccattgaagc ggcgggcgcg accattatca acaccggaat aggctggcac gaagcgcgga
    44881 ttccgaccat cgccacgccc gtgccgcgcg gcgcatttag ctgggtcacg cgcaaactga
    44941 aaggccacgt ctcgctgccg ctggtaacca ccaaccggat taacgatccg caggttgccg
    45001 acgatattct ctcgcgcggc gatgccgata tagtatcgat ggcgcgaccg tttctcgcgg
    45061 atgcggaact gctgtcaaaa gcgcaatcag ggcgagccga tgagatcaac acttgtattg
    45121 gctgcaacca ggcctgcctc gatcagatct tcgttggcaa agtcacctcg tgcctggtga
    45181 atcctcgcgc ctgccacgaa accaaaatgc caatccttcc cgccgtgcag aaaaaaaatc
    45241 tggcggtggt cggcgctgga cctgctgggc tggcgtttgc cattaacgcg gcggcgcgtg
    45301 ggcatcaggt aacattgttt gatgcgcaca gcgagatcgg cgggcagttt aatatcgcca
    45361 aacagatccc cggcaaagag gagttttacg aaacgctgcg ctactaccgc cggatgatcg
    45421 aagtgacggg cgtgacgctg aaactcaatc acaccgtgac ggcggatcag ttacaggcgt
    45481 tcgatgaaac gatcctcgcc agtgggatcg tgccgcgcgt tccgcctatc gacgggatcg
    45541 atcatccgaa ggtattgagt tatctcgatg tactgcgcga caaagcgccg gtaggcaaca
    45601 aggttgccat catcggttgt ggcgggattg gttttgatac ggcgatgtat ttaagtcagc
    45661 cgggcgaatc caccagccag aatatcgcca ggttctgtaa tgagtggggg atcgacagca
    45721 gcctacaaca ggctggtggc ttaagcccgc agggaatgca gatcccgcgt agcccacggc
    45781 aaattgtgat gctccagcgc aaagccagca aaccgggaca ggggttaggc aaaaccaccg
    45841 gctggatcca tcgcaccacc ctgctctcgc ggggtgtgaa aatgatccca ggcgtaagtt
    45901 atcagaagat tgacgatgac gggctgcatg tggtgatcaa cggcgaaacg caggtattag
    45961 cagtggacaa tgtggtgatc tgcgcagggc aagagtcaaa ccgcgcgctg gcgcaaccgc
    46021 tgattgatag cgggaaaacg gtgcatttaa ttggcggctg cgatgtggct atggagctgg
    46081 acgcacgacg ggcaattgcc cagggaacac ggctggcgct ggagatttaa aaagtgcgtt
    46141 gaagtgccgg atgcgacgct tgccgcgtct tatccggcct gggagtaacg tgctgaacgt
    46201 aggccggata aggcgttaac gccgcatccg gcaggtaact cccaatctta ccgacgacgc
    46261 cccagcttca ctgttttcag caccacaaat ttattattcg tggcgatggt ggtgcagttg
    46321 ccgaaaatct tcttcagttt atggaagtaa tccaggtgac ggttggcaac gatatacagt
    46381 tcgccgttga ttttcaggca gcggcgggcg tggtggaaca tctcccaggc gacgttatcg
    46441 gtcagcgcat gttgctgatg aaacggcggg ttgcagagca cagcattaaa gcggaaaggc
    46501 tccactccgg agagcgcgtt gttgatcata aactcgcagc gatccaacgc ctctggcatg
    46561 ttggtttcaa cattcattcg gctggaagcg accgccatcg gcgattcatc gacaaacacc
    46621 actttcgcct gcgggttttt atcaagcagc gtcaggccaa taacgccgtt accacagccg
    46681 agatcgacaa tctcaccttc gagattctct ggcagatgtt gcataaagaa gcgtgcgcca
    46741 atatccagcc cgttgcggga gaagacattc gcatggttgt ggatagtcca gtcagtacct
    46801 tccagcttcc agctaacggt ctgcggcgca tcggccagcg gcggttcatt gaaagtgcaa
    46861 ttaatcaggc gcgctttttt ccatgccagc gtagtggtgg tcgggccgag cactttctcg
    46921 aacagctcca gcgtggaagt gtgaatgtca cgggctttgg caccggcaat aatacgtgta
    46981 tccgacgtga ccactttgcg cagcgcacgc agttgctgtt ccagcaatgc cagtgttttc
    47041 ggcactttga tcagcaccac acccggctgt tgcgggtagt cggcggtgct gtcgagaaac
    47101 ttcacgctcg actcatcaat cccgttgagg cgtaaattct cgcgcgtcgc cagttcactg
    47161 atgtatgagt cgccaatgct gtacggctta tgttccgcca gcgcgcagct taacgcacca
    47221 aaggcatcat tcaggatcaa caccgggccg cggatttctg tgtcgtccaa ctgttgcagc
    47281 aaatattcat ccgccgcttc ccacgcctgt agcgggttaa cgtcatccgt cgccggaaaa
    47341 cgttgtagtg tcagtgaacg gaaaccgttg tctaagtggc tcatcggccc tcctgaatga
    47401 taaaatttcg acgttgcccc tgaaaagggt gcgtgagtat accctttttc tctttttcgt
    47461 gggcggttat gaacaatctt acttatcttc agggttatcc cgagcagcta ctttcccagg
    47521 tgcgaacgct gattaacgaa cagcgtctgg gcgatgtgct ggcaaaacgc tatccgggaa
    47581 cacacgacta cgccaccgat aaagccctct ggcaatatac tcaggatctg aaaaatcagt
    47641 ttctgcgtaa tgccccgccg atcaataaag tgatgtatga caataagatc catgtgctaa
    47701 aaaacgcgct cgggttacat accgctgttt ctcgtgtgca gggcggcaag ctgaaagcaa
    47761 aagcagagat ccgcgtcgct actgtgtttc gcaacgcgcc ggaaccgttt ttacgtatga
    47821 tcgtggtgca cgaactggcg cacctgaagg agaaagagca taacaaagcg ttttaccagt
    47881 tgtgctgcca tatggagccg cagtaccacc agcttgagtt cgacacccgg ctgtggctaa
    47941 cgcagttgtc gcttgggcag gacaaaatct gaaaatgatg tcaggcgtta tcttttcttt
    48001 ctttagcgcc taaaatcgac ctcccccctt tcgttaagga taacgaccat gcagccccgc
    48061 cttcttctcc gtatttgttt ttctcgccgg actcttaaga ttggctgttt gttgttgctt
    48121 atagcaggag cgaccatttt tattgccgat cgcgttatgg tcaacgccag caagcagctg
    48181 acctggagcg atgtcaacgc agttccggcg cgtaacgtgg gcttattgct gggggccagg
    48241 ccgggtaatc gctactttac ccgacgtatt gataccgcag cagcgttgta ccacgccgga
    48301 aaagtgaaat ggctactggt aagtggcgat aacggacgta aaaattacga tgaagcctca
    48361 ggtatgcagc aggctttgat cgctaaaggc gtaccggcga aagtgatctt ctgcgactat
    48421 gccggattct caacgctgga ttcggtagtg cgtgccaaaa aggtttttgg tgaaaaccat
    48481 atcaccatta tctcgcaaga atttcataat caacgcgcca tctggctggc aaaacagtac
    48541 ggcatcgacg ctatcggttt taacgcgcct gacctcaata tgaagcacgg tttttatact
    48601 caactgcggg agaaactcgc tcgtgtcagc gccgtgattg acgccaaaat cctccatcgc
    48661 cagccgaaat atctcgggcc atcggtcatg attgggccgt ttagtgagca tggctgtccg
    48721 gcaaaagaat aatgcgtatc tgcgcacgtc gaagatgaaa aaggcgtgct acattgacga
    48781 cagaatccct ttatggagta tccacgcgtt atgatacgtt tcgctgtgat tggtacgaac
    48841 tggatcactc gccagttcgt cgaggccgcc catgagagcg gtaaatacaa gttaaccgcc
    48901 gtatattccc gcagccttga gcaggctcag cacttcgcca atgatttttc tgtcgagcat
    48961 ctgtttacct cgctggaagc gatggcggaa agcgatgcca ttgacgcggt gtatattgcc
    49021 agcccgaatg ccctgcactt ttcccagaca caacttttcc ttagccataa aattcatgtg
    49081 atttgcgaga aaccactggc gtcgaatctg gcggaagtgg atgccgccat tgcctgtgcg
    49141 cgggaaaatc aggtggtgct gtttgaggca tttaaaaccg cctgcctgcc gaactttcat
    49201 ttgctgcgcc aggcgctgcc gaaagtcggc aaattgcgta aagtcttttt caactattgc
    49261 cagtattcct cgcgctatca acgttacctg gacggtgaaa atcccaacac ctttaatccg
    49321 tcattctcta acggttcaat tatggatatc ggcttttact gcctggcgtc ggcggtggcg
    49381 ttatttggtg agccgaaaag cgtgcaggca accgccagtt tgctggcaag cggtgtagat
    49441 gcccacggcg tggtggtgat ggattacggt gatttcagcg tcaccttgca gcactcaaaa
    49501 gtcagtgatt ctgtcctggc gagcgagatt cagggcgaag caggatcgct ggtgattgaa
    49561 aaactgtctg aatgccagaa agtgtgcttc gtgccgcgtg gcagccaaat gcaggatctc
    49621 acccagccgc agcatattaa taccatgctc tacgaagcag agctgttcgc taccctggtg
    49681 gatgagcatc tggtggatca tccggggctg gcggtcagtc gcatcaccgc caaactgctg
    49741 accgagatcc gccgccagac tggggtgatt tttccggcag atagcgtaaa actataattg
    49801 ccaaagtaaa acagtgtaaa aggtatgtaa cagaccattg actggctgaa tggtctgtca
    49861 tactttgtta cctgcaaagg ggagtaactt cattgccggt cgatcgtcat tacgatgtgt
    49921 gaaaaaccac atccggtcac cgggcaaccc gaaaggaata cgcagacgta ttcctttttt
    49981 gttgtaagtg agaccttgcc ggaaggcgag gtctatgcat aaaaagcagc ggctgacgtc
    50041 ttccgacgtt ggccgttttt ttatgtgtaa ggaacttcta tgaatactgt cggcacgccg
    50101 ttgctatggg gcggattcgc tgttgttgtc gccattatgc tggctatcga cctgttgttg
    50161 caggggcgtc gtggggcaca tgccatgacc atgaaacagg ctgcggcctg gtcgctggtc
    50221 tgggtgacgc tgtcgttact gtttaacgcc gctttctggt ggtatctggt gcaaactgaa
    50281 ggtcgcgccg ttgccgatcc acaggcactg gcctttctca cgggctatct gattgagaaa
    50341 tcgctggcgg tcgataacgt ctttgtctgg ctgatgttgt tcagctattt ctctgttccg
    50401 gcggcattac aacgccgcgt gctggtgtat ggcgtgctcg gggcgattgt tctgcgtacc
    50461 atcatgatct tcacaggcag ctggctgatt tcacagttcg actggatcct gtatatcttc
    50521 ggtgccttcc tgctgtttac cggcgtgaag atggcgctcg cccatgaaga tgaatcaggc
    50581 attggcgaca agccgctggt gcgctggcta cgcggtcatt tgcgcatgac cgacaccatc
    50641 gacaacgagc atttctttgt gcgtaagaat ggattgctgt acgccacacc gctgatgctg
    50701 gtactgattc tggtggaatt gagcgacgtg attttcgccg tggatagcat tccggctatc
    50761 ttcgccgtga ccactgaccc gttcattgtg ctgacctcaa acctgtttgc gatcctcggc
    50821 ctgcgtgcga tgtatttcct gctggcgggc gtagcagagc gtttctcgat gctcaaatat
    50881 ggcctggcgg tgattctggt gtttatcggt atcaagatgc tgattgtcga cttctaccat
    50941 attccaatcg ccgtctcgct gggcgtggtg tttggcattc tggtgatgac gtttattatc
    51001 aacgcctggg tgaattatcg gcatgataag cagcggggag aatgatccgc tgtatattaa
    51061 gccattacta gagataactc attccagaca aaggtgagct attaattatg taatcggctt
    51121 atagctcttt acaaaaagac cgcacgggtg ttggtggcgt caaaatcgcc gaagcgttcc
    51181 ctggaggccg gggcgacgcg catggatgcg cggcgagggc gcatcttaca gggacgttac
    51241 cttgcgcccg acccggtagc cgcaagggat aagctgaggg aaccgcgcag cggcgatttt
    51301 gttcgccaga gcctggggg
//
LOCUS       DAPHDW010000015        50913 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.015, whole genome shotgun sequence.
ACCESSION   DAPHDW010000015 DAPHDW010000000
VERSION     DAPHDW010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50913
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.015"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1724)
                     /gene="rsxC"
                     /locus_tag="RH057_000848"
     CDS             complement(<1..1724)
                     /gene="rsxC"
                     /locus_tag="RH057_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HDV3127261.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQSNGTPLRQVPLAQRFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWADYRTRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPADLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKAARLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAAREARKAQARAKQAELQQTNDAATVADPRKTAVEAAIARA
                     KARKLEQQ"
     gene            complement(1717..2295)
                     /gene="rsxB"
                     /locus_tag="RH057_000849"
     CDS             complement(1717..2295)
                     /gene="rsxB"
                     /locus_tag="RH057_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HDV3127262.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     ITPARMVAVIDENNCIGCTKCIQACPVDAIVGATRAMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            complement(2295..2876)
                     /gene="rsxA"
                     /locus_tag="RH057_000850"
     CDS             complement(2295..2876)
                     /gene="rsxA"
                     /locus_tag="RH057_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HDV3127263.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLASICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAIGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            complement(2953..3393)
                     /locus_tag="RH057_000851"
     CDS             complement(2953..3393)
                     /locus_tag="RH057_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HDV3127264.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEDGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            complement(3479..3694)
                     /gene="ydgT"
                     /locus_tag="RH057_000852"
     CDS             complement(3479..3694)
                     /gene="ydgT"
                     /locus_tag="RH057_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HDV3127265.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            complement(3967..4092)
                     /gene="blr"
                     /locus_tag="RH057_000853"
     CDS             complement(3967..4092)
                     /gene="blr"
                     /locus_tag="RH057_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HDV3127266.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            4335..5375
                     /locus_tag="RH057_000854"
     CDS             4335..5375
                     /locus_tag="RH057_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV3127267.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGEQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            complement(5410..6411)
                     /gene="add"
                     /locus_tag="RH057_000855"
     CDS             complement(5410..6411)
                     /gene="add"
                     /locus_tag="RH057_000855"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HDV3127268.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARHGLHYVELRFSPG
                     YMAMAHQLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(6515..7687)
                     /gene="malY"
                     /locus_tag="RH057_000856"
     CDS             complement(6515..7687)
                     /gene="malY"
                     /locus_tag="RH057_000856"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HDV3127269.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADDWFCDMGKLEAVLAK
                     PECKIMLLCSPQNPTGKVWTCDELEIMADLCERHGVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            complement(7697..9289)
                     /gene="malX"
                     /locus_tag="RH057_000857"
     CDS             complement(7697..9289)
                     /gene="malX"
                     /locus_tag="RH057_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HDV3127270.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLIGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESATRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVKDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVRA"
     gene            9464..10492
                     /gene="malI"
                     /locus_tag="RH057_000858"
     CDS             9464..10492
                     /gene="malI"
                     /locus_tag="RH057_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HDV3127271.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLSGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGTTLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            10604..11371
                     /gene="hdhA"
                     /locus_tag="RH057_000859"
     CDS             10604..11371
                     /gene="hdhA"
                     /locus_tag="RH057_000859"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HDV3127272.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLGGQAFACRCDITSEQELSALADFAVSKLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNGIAPGAILTDALKSVITPEIEQKMLQHTPIRRL
                     GQPQDIANAALFLCSPAASWVSGQILTVSGGGVQELN"
     gene            11600..12190
                     /gene="uidR"
                     /locus_tag="RH057_000860"
     CDS             11600..12190
                     /gene="uidR"
                     /locus_tag="RH057_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HDV3127273.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTSMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            12578..14389
                     /gene="uidA"
                     /locus_tag="RH057_000861"
     CDS             12578..14389
                     /gene="uidA"
                     /locus_tag="RH057_000861"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HDV3127274.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWWESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMVYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDVSVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     KKGIFTRDRKPKSAAFLLQKRWTGMNFGEKPQQGGKQ"
     gene            14386..15759
                     /gene="uidB"
                     /locus_tag="RH057_000862"
     CDS             14386..15759
                     /gene="uidB"
                     /locus_tag="RH057_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
                     /protein_id="HDV3127275.1"
                     /translation="MNQQLSWRTIVGYSLGDVANNFAFAMGALFLLSYYTDVAGVGAA
                     AAGTMLLLVRVFDAFADVFAGRVVDSVNTRWGKFRPFLLFGTAPLMIFSVLVFWVPTD
                     WSHSSKVVYAYLTYMGLGLCYSLVNIPYGSLATAMTQQPQSRARLGAARGIAASLTFV
                     CLAFLIGPSIKNSSPEEMVSVYHFWTIVLAIAGMVLYFICFKSTRENVVRIVAQPSLK
                     ISLQTLKRNRPLFMLCIGALCVLISTFAVSASSLFYVRYVLNDTGLFTVLVLVQNLVG
                     TVASAPLVPGMVARIGKKNTFLIGALLGTCGYLLFFWVSVWSLPVALVALAIASIGQG
                     VTMTVMWALEADTVEYGEYLTGVRIEGLTYSLFSFTRKCGQAIGGSIPAFILGLSGYI
                     ANQVQTPEVIMGIRTSIALVPCGFMLLAFVIIWFYPLTDKKFKEIVVEIDNRKKVQQQ
                     LISDITN"
     gene            15798..17063
                     /gene="uidC"
                     /locus_tag="RH057_000863"
     CDS             15798..17063
                     /gene="uidC"
                     /locus_tag="RH057_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HDV3127276.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIIGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMAGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWHDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQVTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGVN
                     YRLTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(17108..18616)
                     /locus_tag="RH057_000864"
     CDS             complement(17108..18616)
                     /locus_tag="RH057_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV3127277.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLK
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLASFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATEFMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYTNGQITLNGQKMPLEDFVGMFAMPALNVPVVPAIPQQ"
     gene            complement(18717..19892)
                     /gene="manA"
                     /locus_tag="RH057_000865"
     CDS             complement(18717..19892)
                     /gene="manA"
                     /locus_tag="RH057_000865"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416130.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HDV3127278.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESNKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     RNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPNAERLSELFASLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            20091..21737
                     /gene="fumB"
                     /locus_tag="RH057_000866"
     CDS             20091..21737
                     /gene="fumB"
                     /locus_tag="RH057_000866"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV3127279.1"
                     /translation="MSNKPFHYQAPFPLKKDDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGCDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            21880..23283
                     /gene="fumC"
                     /locus_tag="RH057_000867"
     CDS             21880..23283
                     /gene="fumC"
                     /locus_tag="RH057_000867"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HDV3127280.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLGLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(23280..24209)
                     /gene="tus"
                     /locus_tag="RH057_000868"
     CDS             complement(23280..24209)
                     /gene="tus"
                     /locus_tag="RH057_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HDV3127281.1"
                     /translation="MARYDLVDRLNTTFRQMEQELAAFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(24285..25586)
                     /gene="rstB"
                     /locus_tag="RH057_000869"
     CDS             complement(24285..25586)
                     /gene="rstB"
                     /locus_tag="RH057_000869"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HDV3127282.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLAQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALAMGGTVNCDTSELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(25590..26309)
                     /gene="rstA"
                     /locus_tag="RH057_000870"
     CDS             complement(25590..26309)
                     /gene="rstA"
                     /locus_tag="RH057_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HDV3127283.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     SPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWE"
     gene            26438..26773
                     /locus_tag="RH057_000871"
     CDS             26438..26773
                     /locus_tag="RH057_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HDV3127284.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(26770..27492)
                     /gene="folM"
                     /locus_tag="RH057_000872"
     CDS             complement(26770..27492)
                     /gene="folM"
                     /locus_tag="RH057_000872"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HDV3127285.1"
                     /translation="MGKAQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLIKAGAQCIQADFSTNDGVMAFADEVLKSTHGLRAILHNASAWMAEKPGTPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(27529..28911)
                     /locus_tag="RH057_000873"
     CDS             complement(27529..28911)
                     /locus_tag="RH057_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3127286.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(29097..30041)
                     /locus_tag="RH057_000874"
     CDS             complement(29097..30041)
                     /locus_tag="RH057_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3127287.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQERGNNLTVSADLYK"
     gene            30565..32097
                     /gene="pntA"
                     /locus_tag="RH057_000875"
     CDS             30565..32097
                     /gene="pntA"
                     /locus_tag="RH057_000875"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HDV3127288.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYTVPGEIFTTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLASVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            32108..33496
                     /gene="pntB"
                     /locus_tag="RH057_000876"
     CDS             32108..33496
                     /gene="pntB"
                     /locus_tag="RH057_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HDV3127289.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(33521..34555)
                     /gene="tqsA"
                     /locus_tag="RH057_000877"
     CDS             complement(33521..34555)
                     /gene="tqsA"
                     /locus_tag="RH057_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HDV3127290.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            34967..35332
                     /gene="mdtJ"
                     /locus_tag="RH057_000878"
     CDS             34967..35332
                     /gene="mdtJ"
                     /locus_tag="RH057_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HDV3127291.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            35319..35648
                     /gene="mdtI"
                     /locus_tag="RH057_000879"
     CDS             35319..35648
                     /gene="mdtI"
                     /locus_tag="RH057_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HDV3127292.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVAYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(35687..36508)
                     /locus_tag="RH057_000880"
     CDS             complement(35687..36508)
                     /locus_tag="RH057_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HDV3127293.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(36608..36691)
                     /locus_tag="RH057_000881"
     CDS             complement(36608..36691)
                     /locus_tag="RH057_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127294.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(36784..37119)
                     /locus_tag="RH057_000882"
     CDS             complement(36784..37119)
                     /locus_tag="RH057_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HDV3127295.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHSHQQP
                     AKPAAQPAA"
     gene            complement(37516..38769)
                     /locus_tag="RH057_000883"
     CDS             complement(37516..38769)
                     /locus_tag="RH057_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3127296.1"
                     /translation="MSRTTTVDGAPASDTDKQSISQPNQFIKRGTPQFMRVTLALFSA
                     GLATFALLYCVQPILPVLSQEFGLTPANSSISLSISTAMLAIGLLFTGPLSDAIGRKP
                     VMVTALLLASICTLLSTMMTSWHGILIMRALIGLSLSGVAAVGMTYLSEEIHPSFVAF
                     SMGLYISGNSIGGMSGRLISGVFTDFFNWRIALAAIGCFALASALMFWKILPESRHFR
                     PTSLRPKTLFINFRLHWRDRGLPLLFAEGFLLMGSFVTLFNYIGYRLMLSPWHVSQAV
                     VGLLSLAYLTGTWSSPKAGTMTTRYGRGPVMLFSTGVMLFGLLMTLFNSLWLIFAGML
                     LFSAGFFAAHSVASSWIGPRAKRAKGQASSLYLFSYYLGSSIAGTLGGVFWHNYGWNG
                     VGAFIALMLVIALLVGTRLHRRLHA"
     gene            38876..39769
                     /locus_tag="RH057_000884"
     CDS             38876..39769
                     /locus_tag="RH057_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3127297.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPHDHPLANNPNVTLAELAKEPFVFFDPHVGTGLYDDILGLMRRYHL
                     TPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSEMWLVWP
                     KHHEQSPAARNFRIHLLNALR"
     gene            39904..41124
                     /gene="mlc"
                     /locus_tag="RH057_000885"
                     /gene_synonym="dgsA"
     CDS             39904..41124
                     /gene="mlc"
                     /locus_tag="RH057_000885"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HDV3127298.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVQELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDSPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGAGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIITGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            41249..41944
                     /gene="bioD"
                     /locus_tag="RH057_000886"
     CDS             41249..41944
                     /gene="bioD"
                     /locus_tag="RH057_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV3127299.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKTVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TEKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(41897..43189)
                     /gene="clcB"
                     /locus_tag="RH057_000887"
     CDS             complement(41897..43189)
                     /gene="clcB"
                     /locus_tag="RH057_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HDV3127300.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAAFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSPWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAVVALLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(43349..43963)
                     /gene="dmsD"
                     /locus_tag="RH057_000888"
     CDS             complement(43349..43963)
                     /gene="dmsD"
                     /locus_tag="RH057_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HDV3127301.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(44006..44860)
                     /locus_tag="RH057_000889"
     CDS             complement(44006..44860)
                     /locus_tag="RH057_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HDV3127302.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQRIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAGVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLILGLILILGGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(44862..45479)
                     /gene="dmsB"
                     /locus_tag="RH057_000890"
     CDS             complement(44862..45479)
                     /gene="dmsB"
                     /locus_tag="RH057_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV3127303.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(45490..47913)
                     /locus_tag="RH057_000891"
     CDS             complement(45490..47913)
                     /locus_tag="RH057_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase subunit A"
                     /protein_id="HDV3127304.1"
                     /translation="MKIHTTEALMKAEISRRSLMKTSALGSLALASSAFTLPFSQMVR
                     AAEAPVEEKAVWSSCTVNCGSRCLLRLHVKDDTVYWVESDTTGDDVYGNHQVRACLRG
                     RSIRRRMNHPDRLKYPMKRVGKRGEGKFERISWDEALDTISDNLRRILKDYGNEAVHV
                     LYGTGVDGGNITNSNVPYRLMNSCGGFLSRYGSYSTAQISAAMSYMFGANDGNSPDDI
                     ANTKLVVMFGNNPAETRMSGGGVTYYVEQARERSNARMIVIDPRYNDTAAGREDEWLP
                     IRPGTDGALACAIAWVLITENMVDQPFLDKYCVGYDEKTLPANAPRNAHYKAYILGEG
                     PDGIAKTPEWAAKITSIPAEKIIQLAREIGSAKPAYICQGWGPQRHSNGEQTSRAIAM
                     LSVLTGNVGINGGNSGVREGSWDLGVEWFPMLENPVKTQISVFTWTDAIDHGTEMTAT
                     RDGVRGKEKLDVPIKFLWCYASNTLINQHGDINHTHEVLQDDSKCEMIVGIDHFMTAS
                     AKYCDILLPDLMPTEQEDLISHESAGNMGYVILAQPATSAKFERKPIYWMLSEVAKRL
                     GPDVYQTFTEGRSQHEWIKYLHAKTKERNPEMPDYEEMKTTGIFKKKCPEEHYVAFRA
                     FREDPQANPLKTPSGKIEIYSERLAKIADTWELKKDEIIHPLPAYTPGFDGWDDPLRK
                     TYPLQLTGFHYKARTHSSYGNIDVLQQACPQEVWINPIDAQARGIRHGDTVRVFNNNG
                     EMLIAAKVTPRILPGVTAIGQGAWLKADMFGDRVDHGGSINILTSHRPSPLAKGNPSH
                     SNLVQIEKV"
     gene            complement(48015..49628)
                     /locus_tag="RH057_000892"
     CDS             complement(48015..49628)
                     /locus_tag="RH057_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISEc23 family transposase"
                     /protein_id="HDV3127305.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     TRAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVELAEALLKQREQDSDRYS
                     GREDDPQVPRQLRQSRHRRPLPAHLPREIHRLEPEESCCPECGGELDYLGEVSAEQLE
                     LVSSALKVIRTERVKKACTKCDCIVEAPAPSRPIERGIAGPGLLARVLTGKYCEHLPL
                     YRQSEIFARQGVELSRALLSNWVDACCQLMTPVNDALYRYVMNTRKVHTDDTPVKVLS
                     PGQKKAKTGRIWTYVRDDRNVGSSSPPAVWFAYSPNRQGKHPEQHLRPFRGILQADAF
                     TGYDRLFSAEREGGALTEVACWAHARRKIHDVYISSKSATAEEALKRISELYAIEDEI
                     RGLPESERLAVRQQRSKVLLTSLHEWMVEKNGTLSKKSRLGEAFSYVLNQWDALCYYS
                     DDGLAEADNNAAERALRAVCLGKKNFMFFGSDHGGERGALLYGLIGTCRLNGIDPEAY
                     LRHILSVLPEWPSNRVDELLPWNVVLTNK"
     gene            complement(49659..50009)
                     /gene="tnpB"
                     /locus_tag="RH057_000893"
     CDS             complement(49659..50009)
                     /gene="tnpB"
                     /locus_tag="RH057_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3127306.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQVQHVLNDNPFSGHLF
                     IFRGRRGDTVKILWADADGLCLFTKRLEEGQFIWPAVRDGKVSITRSQLAMLLDKLDW
                     RQPKTSSRNSLTML"
     gene            complement(50006..50431)
                     /locus_tag="RH057_000894"
     CDS             complement(50006..50431)
                     /locus_tag="RH057_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV3127307.1"
                     /translation="MELQDWRKEPRKNYSNEFKLRMVELASQPGACVAQIARENGVND
                     NVIFKWLRLWQNEGRVSRRLPVTTSSDTGVELLPVEITPDEQKEPVAAIAPSLSTSTQ
                     TRVSASSCKVEFRHGNMTLENPSPELLTVLIRELTGRGR"
     gene            complement(50518..50913)
                     /locus_tag="RH057_000895"
                     /pseudo
     CDS             complement(50518..50913)
                     /locus_tag="RH057_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT        12743 a        12683 c        13142 g        12345 t
ORIGIN      
        1 tgttgctgtt ccagcttgcg cgctttggcg cgggcgatag ctgcttcaac ggcagtttta
       61 cgtggatcag caacggttgc tgcgtcgtta gtttgctgca gttctgcctg tttcgctctg
      121 gcttgcgctt tacgggcttc ccgtgctgca ataattgcac tgttatccgg gcgttcgccc
      181 gctttaatca caataggctg tgtagcctgg gcctgtttct ctttcacccg cgccagagcg
      241 gcagcaatcg catctttatc tttggctgca ggttgaacgg ctgcgctctt atgtcgttca
      301 aggcgagccg ctttttcgcg ctccagacga gcctggcgcg cttcgaaacg cgctttggct
      361 tctgcggcgc gcttttcttc ctgacgaata gccgcaattt cagctttttc ctgacggaaa
      421 tattgcacca ggggaatatt gctcgggcaa acccacgcgc aagccccaca ttcaatgcaa
      481 tcagcaatgt tatgcgtggt agctttatcg tgttgctgac ctttgctgaa ccagtacaac
      541 tgttgcggca aaagatccgc agggcaggcg tcagcacagg cgctacaccg gatgcagctt
      601 tgttcttcct gtggttcgcc aagttcattg gcagagggag ccaacagaca gttggtaatc
      661 tttacgaccg ggacatccag ccatggcaag gtaaagccca ttagcgggcc acccataatc
      721 accatttgat cggcagaggg gcagaatccg gcatcattca ataaatgacg cactggcgtc
      781 cccagccgtg cccagacgtt gcccgggcga gcgattgctt cgccagtcag ggttacaaca
      841 cgctcggtaa tcggctcgcc atcaataacg gcacgtttca ctgcataagc agtgccgacg
      901 ttttgcatta atacgccgat atcggatgaa cgcccgccat gtggaacctg cttcccggtc
      961 agaatgtagg ttaattgttt agcaccgcca gaaggatatt tggttggaat cacccgcaga
     1021 gaaatatcgt tagagtccgc cagcaccgcg cgcagcatgg aaatcgcctg cggtttgtta
     1081 tcttcaatgc cgataagaat ttcgcgtggc tgcagaatat gcgcaagaat gcgaataccc
     1141 tctacgacct gagccgcgca atcctgcatc aaacggtcat cggcggtaat gtacggctcg
     1201 cactcagccg cgttgataat caacgtttca atcttatctc cgccaccctg caatttaacg
     1261 cctgtcggga atcctgcacc gcccagcccg gcaacaccaa actgatgtat gcgctcgatt
     1321 aactcttcgc gactgcgagt gcgataatcg gcccagccgt cgcgcgggat ccagcagtct
     1381 tcaccatcgg catcaataat cacgcttaat tcagctaaag ctgaaggatg agccgtagag
     1441 tggggcgcaa tagccgtaac ggtacccgag gtgggcgcgt gaacaggcag cattttgccg
     1501 cgaccacggg taagcggctg gccgcgcaat actttatcgc cgacgctaac gcacaactca
     1561 ccttcagcgc caatatgctg tttcagtgga ataacaaaac gctgcgccag tggtacctgg
     1621 cgcaggggtg taccgttgga ctgggttttc atctccggtg gatggatgcc gccgttgaaa
     1681 tcccagattt tattttttct gaatgcagag aataacttaa gcatggtgtt ccacgggaat
     1741 gatacgcacg ggaatggtgt tcagatccca tttccaggag tcaggtgttt ctgcgaccgg
     1801 ttgcaacgag atgcagtgcg tcgggcacgg atcaacacat aaattgcagc ccgtacagag
     1861 atcactcatt accgtatgca tggcacgggt agcgccaacg atggcgtcta ccggacacgc
     1921 ctgaatacat ttagtgcagc caatacagtt attttcatca ataaccgcca ccatccgcgc
     1981 aggcgttatc tcttgcgctt cgccatccag cggctgcggc tcgacattaa gcaactcggc
     2041 aatttttagc atcacagctt cgccacctgg ggcgcaacgg ttgatttttt caccgttaca
     2101 gctgatggct tccgcgtagg ggcgacagcc gggataaccg cactgaccac actggctctg
     2161 cggtaagatt tcgtcaattt tctcaacgac cggatcgtct tccaccgcaa aacggcggga
     2221 ggcataaccc agaatggcgc caaacgccag gcccagcagg ctcacggcgg caacggcaat
     2281 ccagatagca ttcattacaa cttcaccaaa ccactaaagc ccataaaggc cagagacata
     2341 agacctgcgg taattaacgc aatggcatta ccgcgaaaag gtgccgggac atcagccaca
     2401 gcaaggcgtt cgcggatggc ggcgaagagc accatcacca gcgagaaacc gatagcggcg
     2461 gaaaaaccgt acagcgccga ctgcaagaaa ttgtgcccga gattgatatt cagcaacgcc
     2521 acgccgagca ctgcacagtt ggtggtgata agcggcaaaa aaatccccag cagacggtaa
     2581 agcaccgggc tggttttgcg caccaccatc tcggtgaact gcacgaccac agcaatcacc
     2641 agaataaatg ccagggtgcg caggtaaatc agattaagtg ggatcaaaat ccacgtatcg
     2701 ataagccagg cgcaaataga cgccagcgtc atcacaaacg ttgttgccag ccccatgccc
     2761 atcgcggttt ccagtttttt ggaaaccccc ataaacggac agagaccgag aaacttgacc
     2821 agtacaaagt tattgaccag tacagttccg acaaacagta acaggtagtc agtcattttt
     2881 cggcctgaaa taaaaaagcc gcctattatc cgttaatcca gagcaggcga caacaggtta
     2941 actgtaaggt tattacggat tcacaaacgt cgcttttacc cgcgacgagc gtttgaaata
     3001 ggggacgatc aatgcggttg ccaacaaagt aaacagcaac tggcgaacgg ctatgccatc
     3061 ttccacgggt gaaaaagcga atgcttttac tgccagcaat acggaaataa gcagccagat
     3121 aatatagtgt ttaggaacac aacgccgacg tttaaagaaa gcgatggtca accacagtgt
     3181 gtaataccac atggcgatag cggtaataaa agaaacgccc cataagattt gtgtcgtcag
     3241 tgcttgtccg ccgagcgttt gaaatgtttg cggagaagat aacgcagcgg tatacagcaa
     3301 caacgccagc gtcgtactca acagagcgac taaaagccag gccaacgggc caagcaacca
     3361 gcctccaata cgttgtggcg ttgtggtggt cataggttct ccagaatagc gacgcaaaca
     3421 ggcaaatcta attttagggg cgttattata aggcttttat aaccgatcgc tactttcttt
     3481 attggacata gtgccagacg gacttcggta cctggccgag gtcaaacaaa cgcccgccag
     3541 aaaccagctc tgcgcgacgg tgatcggcag cacgatacat attgatcagt tcctgatcgt
     3601 ccgtcagggt gtaattaaga tggtcgtaga gtttttccag actttcgagt gaactgattt
     3661 tgcgaaattt taataagtag tcctgaacag tcataaaagg tccataaaat aattaatacc
     3721 aaaggggtag gtattattag tgatgataaa atttcaccaa aagtgaacgc ttgtgttatt
     3781 acaataataa gaatgcgctg atggcaaata ttcagggatg aaaaactgct ctgaaaagag
     3841 taacgcgctt tactatccct gacaatcacc aacaacatcg aacaagataa taaattcctg
     3901 gtttaatatc cgacaagtga aaacatgcac ccggacgggc agcatgtcgc tccacaagtg
     3961 cagagcttac ttgtgttgta ccgaagcact ctgttcaggt ggctgatagt tgtcaatgtg
     4021 actcgccacg ccaagaagaa tgactgaaac gacaagaacg atccaacctg ttaattcaat
     4081 aagacgattc attacagccc acatctctct tgattgatcc attaacttca gggggtaaat
     4141 gttacttagc aataatagct ccagcaagat ttttactgag gtttttgcga tatttagctt
     4201 ttgtcgttgg aaaattcgct atttttttga cttaagttaa acaacatccc ttattgctgg
     4261 caggttatta aactgttgag cgtgggtaaa ggatagtgtc aaatagccat catacttcaa
     4321 tgagaggcaa tgacatgagc gacaacatcc gtgttgggtt gattgggtat ggttatgcga
     4381 gcaaaacctt ccatgcgccc ctgattgcgg gcacgcccgg gctggaactg gcggtaatct
     4441 ccagcagtga tgaaacaaaa gtaaaagccg actggccaac ggttacggtt gtctctgagc
     4501 cgaagcatct gtttaacgat cccaacatag acctgattgt cattcctaca cccaacgata
     4561 cccatttccc gttagccaaa gcggcgcttg aggcgggtaa acatgtggtc gttgataaac
     4621 cctttaccgt gacactgtca caagcgcgag agctggatgc gctggcaaaa agcctggggc
     4681 gtgtgctgtc tgtattccat aaccgtcgct gggatagcga tttcctgacg ctaaaaggtt
     4741 tgctcgcgga aggcgtgctg ggtgaagttg cttactttga gtctcatttt gaccgcttcc
     4801 gtccgcaggt gcgcgatcgt tggcgtgaac agggcggccc aggcagcggt atctggtacg
     4861 atttagcacc acatcttctt gatcaggcca ttacgctttt tggtttaccg gtcagcatga
     4921 cggtagattt ggcacagtta cggcccggag agcagtcgac cgattatttc cacgccatct
     4981 tgtcctatcc acagcggcga gtcattttac acggtaccat gctggcagcc gctgagtcag
     5041 cacggtatat cgtgcatgga tcccgaggca gttatgtgaa atatggcctc gatccacagg
     5101 aagaacgtct gaaaaatggc gagcgtctac cgcaggaaga ctggggctac gatatgcgtg
     5161 atggcgtact tacccgcgtg gaaggtgagg aacgtgtcga agaaacgctg ttgacggtgc
     5221 ctgggaatta tccggcttac tatgcggcta ttcgtgatgc gttaaatggc gatggtgaaa
     5281 atccggttcc ggcaagccag gcaatccagg taatggagtt gattgagctg ggcatcgaat
     5341 ccgccaaaca tcgcgcgact ttgtgccttg catgaaaaat ggatggtgca atttgcacca
     5401 tccattttgt tacttcgcgg cgactttttc tcgcagtgcg cgtttttcct cagcgctgag
     5461 gaaagccatt tccagaccat taatctgtgc ctggcggatt tgctcgcggg ataacccagc
     5521 agctggcgcg gcaacggtat attcgtgaat gatatccact ccctgtacgc cgggatcgtc
     5581 agtgttaatg ctggcacgaa tgccatgctc aaggaacgtt ttcagcggat gtgcagccag
     5641 ctctgctacg gtgctggtct gaatattgga ggtcagacag gattcaatac caatttgttg
     5701 ctcggcgaga aaatccatca gcgcccgatc ttcaatggct tttacgccat gtccaatacg
     5761 ctccgcaccc agttcacgaa tcgcctgcca gatgctttcc ggcccggcag cttcgcctgc
     5821 atggacggta atatgccagc ccgcatcacg cgcgcggttg aagtgagaaa ggaacagact
     5881 tcccgggaaa ccaagttcat caccggctaa atcaagtgcg gtaatctggt cacggtgggc
     5941 taaaaaggcc tccagctctt gctgacaggc ggcttcgccg aaggtccggc tcataatgcc
     6001 gataagcttc gcctgcacac caaaggtgcg gcaaccttca cgtacgccat cgatcaccgc
     6061 ttcgacaaca cccgctacag gcagctgatg tgccattgcc atgtagcctg gtgaaaaacg
     6121 cagctcgaca tagtgcaggc cgtgacgggc tgcatcttca atgttttcaa atgccacgcg
     6181 gcgacaggca tcaagagagg cgagaacttt aacgccccag tcaagtttgg tcagaaagct
     6241 caccagatcg ggttcgttgg caatgacctg aacgtgggga atcagtgttt ccagggattg
     6301 tgcaggaagc gagatattat actggcggcc aagttcaaga atggtctggg gacgaatgtt
     6361 gccatcaagg tggcgatgga tatcagttaa tggcagggtg gtatcaatca tggtcgcact
     6421 ctttttctga atataaaagt gcgcaccatt ataaaaacaa agcagggtaa aaatccacga
     6481 ttgcgcaacg attttttacg ttgcgcaatt ggggttagcg aacagcgcgg atggcgttta
     6541 ttaatccagc cacacctttt tccagtttcg aacgtgggca gccggcattg agacggacaa
     6601 aaccacgacc ttcttcacca taggtatacc ccggcatgat cgcgactttt tcttgctcga
     6661 taagcgcttt ttgcaacgcg ttgtcgtcaa tattcaacgg acgtagatca agccaggcca
     6721 gataagtgga ttgcgggatc tgccagttga gttcaggaaa cgcggcgttc attttatctg
     6781 agatatacgt caggttatct ttcagataga cgcgtaaggc atccagccac ggtgcacctt
     6841 gctgataggc ggcgatatgg gcagttaacg ccagtaccga aggggaagaa agcccatcac
     6901 gtcctttcag tgccgataaa taggcatcac ggctgctgct attttctata atcccgtaag
     6961 caccggtcag cgcgggaata ttgaaacttt tcgagcccga cgtcagcaac gcccagtctc
     7021 cccgcgccac attactccag ggaatatgcg gctgctcgcc ccagaccata tccatatgga
     7081 tttcatcgga aataacccgc acaccatgac gctcgcacag gtcagccatg atctccagct
     7141 catcgcacgt ccacactttc ccggtaggat tctgtgggct acacaggagc ataattttac
     7201 attctggttt cgccaacacg gcttccagct tgcccatatc gcaaaaccag tcgtcagcct
     7261 gcttctctaa agcaacgggc attactgtgc gctggttacc ttcaatggcc ttgtaaaatg
     7321 cgtcataggc gggcgtgtgg atcaccacgc cttcacctgt ttcagaccac tgacgaatca
     7381 gttctgaaac catatagatg acagaagggc catacaccac cgtctgcgta tcaattgcgg
     7441 tgtaatgctg ggtggaaaac cagtgggcaa tagccgcgag aaactcatcg tttttccagc
     7501 ggctgtagcc aaatacgccg tgcatcaggc gctgattcag cgcctcgata atgcaggggg
     7561 cagtggcaaa atccatgtct gaaatcgtga acggtaacag gtcagcagtg ccgaaacggt
     7621 cagcgacata atcccactgt gtacaccatg tgccatgacg atccacgacc tttgaaaaat
     7681 cgaacatatc ttatccttat gcccggacag tatgcatcag accggccatt tcatctttta
     7741 ctgactgaac ttgtggcccg ataacaacct gcaggttatg ttgattaagt tgtactacgc
     7801 caattgcccg attgtccttc agtgcctgca cattaacaag cgacatatct ttcacagaca
     7861 aacgcagacg ggtaatgcag ttatcgaggc tgacaatatt gtcggcaccg cctaatgctt
     7921 cgaggattgc aggaacgttg taacctgatt tacccggcgc accggcaacg gctttttcga
     7981 ttgagctggc aacttcgcta tcgcgccccg gggttttcag attgaagcgg gtgatagcga
     8041 aacggaagat gacgtagtaa acgacaaacc agattgccgc caccactggc accatgtacc
     8101 acttggttga cagaccatgc aaaataccga acaccacgaa gtcgatgata ttgccgtcgg
     8161 tattaccgat ggtgacgccg agcacagaca tgacagtgaa gccgaggccg gttaacagcg
     8221 cgtggatgac atacagaact ggcgccacga acaggaacag gaattccagc ggttcggtcg
     8281 tgccgccaac gacacaggcg atcaggccag aaatcagcag acctttaatt ttatggcgat
     8341 tttccgggcg cgcgcagtga tacatagcta acgctgcacc tggcagaccg ccgagaaacg
     8401 caggcatttt accttgcgaa aggaaacgcg tggcgctttc agaaaaaccg tgagtggtcg
     8461 ggcaactcaa ttgcgcctgg aagatggtca gtgcgccgct gacggtttga ccgcagactt
     8521 cctgcgtgcc gcctgcgtcg gtaaagcgaa ttaatgccac cagaatgtga tgcagaccaa
     8581 acggcaacag cagacgttca ccggtaccaa acagcatcgg tccgaaatca cccgcgctgt
     8641 ttatcatatg gcccaagccg ctaataccca tggcgaaaat cggccagact aatggaatca
     8701 ccaggccgat aaggcccatc accagcgagg agataattgg tacgaagcgc gtaccgccga
     8761 agaatgccag cgcatccggc aggcggatat tatggaaacg ctcatgcagc atccagacga
     8821 taataccggc gatcaccgca ccgaggatcc cggtatcgat cgactggatc ccaagaatgc
     8881 tctggatgtt attggctttc agaaccgcgg catccgtggt tggcagaatg cctttattgg
     8941 tcaaccagaa gtttaccgcg aggttcatta ccgcataacc gacgaagcca gcgaatgccg
     9001 ctacgccttt attttcgcgt gccaggccca gcgggatggc gatacagaac atgacaggca
     9061 ggaaactaaa agcaaacgag ccaatcttac tcatccaggt aaagatagcc tgcaacacgg
     9121 ggttgcccag gaccgggatc agggttatga catcatggct gctaagagaa ctaccaatgc
     9181 cgagcataat gccgcagaac gacaataatg ccacgggtaa catgaaggtt ttgcctaact
     9241 gctggaagaa ctcccacagc gtgactttcg gtgctgtttt cgccgtcata aaacgactcc
     9301 tcgtagagaa caatctggat tcagtaaatt gcgcgagaag ataaaacgtt ttatcaaatt
     9361 ttagtgaggc ataaatcaca ttacgcaacg ataatagcgg gtataagata aataaaaggt
     9421 aaaacgtttt atctgtcaca taatcaggga gtaggtcatc tgcatggcta ccgccaaaaa
     9481 aataaccatt catgatgttg cgctggctgc gggcgtgtcg gtaagtaccg tttcgctggt
     9541 gcttagtggc aaagggcgaa tctctaccgc cacaggagaa cgcgttaacg ccgccattga
     9601 agagctggga tttgtgcgca atcgccaggc gtcggcgctg cgcggcgggc aaagcggcgt
     9661 cattggtttg atcgtccgtg atttatctgc gccgttttac gccgaattga cggccggatt
     9721 gacggaagct ctggaagcgc agggacggat ggtttttttg cttcacggcg gtaaagacgg
     9781 cgagcagctg gcacagcggt tttcactgtt actgaatcag ggtgtggatg gtgtggtaat
     9841 tgccggggct gcaggaagca gcgatgacct gcgacggatg gcagaagaaa aagctatccc
     9901 ggtgattttc gcttcccgtg ccagttatct tgatgatgtt gatacggttc gcccggacaa
     9961 catgcaggct gcacagttgt tgacggagca tctcattcgc aatgggcatc agcggatcgc
    10021 ctggctggga gggcaaagtt cctcattaac ccgtgcagaa cgggtggggg gctattgtgc
    10081 aactctacta aaatttggcc tgccgtttca cagcgattgg gtgctggagt gcacatccag
    10141 ccagaagcaa gccgcggaag ctatcacggc gcttttacgt cataacccga ccatcagtgc
    10201 tgtggtttgc tataacgaaa ctattgcgat gggggcatgg tttggtttgc tcaaagcagg
    10261 gcggcaaagc ggggaaagcg gagtcgatcg ttactttgag caacaagttt cgctggcggc
    10321 atttaccgat gcgacaccaa ccacacttga tgatataccc gttacctggg ccagcacgcc
    10381 tgcgcgggaa cttggtacca cacttgcgga tcgcatgatg caaaaaatca cccatgaaga
    10441 gacgcattca cgcaatctta ttattcccgc ccggctcatt gcggcgaaat aatcctctct
    10501 ttatctgcta tacctggtag tgtcctttcc tcaaggttaa tgataaaaaa tgtggcacac
    10561 aggcggtgcc ttttattttt actgaagttc acaggaggtt tatgtgttta attctgacaa
    10621 cctgagactc gacggaaaat gcgccatcat cacaggtgcg ggtgcaggta ttggtaaaga
    10681 aatcgccatt acattcgcga cagctggcgc atctgtggtg gtcagtgata ttaacgccga
    10741 cgcagctaac catgttgtag acgaaattca acaactgggt ggtcaggcat ttgcctgccg
    10801 ttgtgatatt acttccgaac aggaactctc tgcactggca gactttgccg tcagtaagct
    10861 gggtaaagtt gatatcctgg ttaacaacgc cggtggcggt ggtcctaaac cgtttgatat
    10921 gccaatggca gattttcgcc gtgcttatga actgaatgtg ttttcttttt tccatctgtc
    10981 acaacttgtt gcgccagaaa tggaaaaaaa tggcggtggc gttattctga ccatcacttc
    11041 tatggcggca gaaaataaaa atataaacat gacttcctat gcatcatcta aagctgcggc
    11101 cagtcatctg gtcagaaata tggcatttga cctgggtgaa aaaaatattc gggtgaatgg
    11161 cattgcgccg ggggcaatat taaccgatgc cctgaaatcc gttattacac cagaaattga
    11221 acaaaaaatg ttacagcaca cgccgatcag acgtctgggc caaccgcaag atattgctaa
    11281 cgcggcgctg ttcctttgct cgcctgcagc cagctgggta agcggacaaa ttctcaccgt
    11341 ctccggtggt ggggtacagg agctcaatta atacactaac gaaccggtaa acagccgtat
    11401 gtgttgttta ccgggatagc gtgtgcaatt catcaacgtc tctgctaaac aactggcagc
    11461 caaatcacgg ctattggtta accaatagct ggatgaaaag catacgaata gagtgtgcct
    11521 tcgcactatt caacagcaat gataggcgct cacctgacaa cgcggtaaac tagttattca
    11581 cgctaactat aatggtttaa tgatggataa catgcagact gaagcacaac cgacacggac
    11641 ccggatcctc aatgctgcca gagagatttt ttcagaaaat ggatttcaca gtgcctcgat
    11701 gaaagccatc tgtaaatcct gcgccattag tcccgggacg ctctatcacc atttcatctc
    11761 caaagaagcc ttgattcagg cgattatctt acaggaccag gagagggcgc tggcccgttt
    11821 ccgggaaccg attgaaggga ttcattttgt tgactatatg gtcgagtcta ttgtctctct
    11881 cacccatgaa gcctttgggc aacgggcgct ggtggttgaa attatggcgg aagggatgcg
    11941 taacccacag gtcgccgcca tgcttaaaaa taagcatatg acgatcactg aatttgttgc
    12001 ccagcggatg cgtgatgccc agcaaaaagg cgagataagc ccagacatca acacgtcaat
    12061 gacttcacgt ttactgctgg atctgaccta cggtgtactg gccgatatcg aagcggaaga
    12121 cctggcgcgt gaagcgtcgt ttgctcaggg attacgcgcg atgattggcg gtatcttaac
    12181 cgcatcctga tcctctcatt tcgcgggctg gtgataactg tgcccgcgtt tcatacttta
    12241 atttccctgt acaaaataca atctagccgg gatcggagaa ttccccccaa aaatattcac
    12301 cgtagccata tgtcatgaga gtttatcgtt ctcaagatgc tcgaacgaac gttcgattgc
    12361 ttattttatg gcttctgtca acgctgtttt aaagattaat gcgatctata tcacgctgtg
    12421 ggtattgcag attttggttt tttgatcgcg gtgtcagttc tttttatttc catttctctt
    12481 ccatgggttt ctcacagata actgtgtgca acacagaatt ggttaactaa tcagattaaa
    12541 ggttgaccag tatttttatt tgaatgagga gtcccttatg ttacgtcctg tagaaacccc
    12601 aacccgtgaa atcaaaaaac tcgacggcct gtgggcattc agtctggatc gcgaaaactg
    12661 tggaattgat cagcgttggt gggaaagcgc gttacaagaa agccgggcaa ttgctgtgcc
    12721 aggcagtttt aacgatcagt tcgccgatgc agatattcgt aattatgtgg gcaacgtctg
    12781 gtatcagcgc gaagtcttta taccgaaagg ttgggcaggc cagcgtatcg tgctgcgttt
    12841 cgatgcggtc actcattacg gcaaagtgtg ggtcaataat caggaagtga tggagcatca
    12901 gggcggctat acgccatttg aagccgatgt cacgccgtat gttattgccg ggaaaagtgt
    12961 acgtatcacc gtttgtgtga acaacgaact gaactggcag actatcccgc cgggaatggt
    13021 gattaccgat gaaaacggca agaaaaagca gtcttacttc catgatttct ttaactatgc
    13081 cgggatccat cgcagcgtaa tggtctacac cacgccgaac acctgggtgg acgatatcac
    13141 cgtggtgacg catgttgcgc aagactgtaa ccacgcgtct gttgactggc aggtggtagc
    13201 aaatggtgat gtcagcgttg aactgcgtga tgcggatcag caggtggttg caactggaca
    13261 aggtaccagc gggactttgc aagtggtgaa tccacacctc tggcaaccgg gtgaaggtta
    13321 tctctatgaa ctgtgcgtca cagccaaaag ccagacagag tgtgatatct acccgcttcg
    13381 cgtcggcatc cggtcagtgg cagtgaaggg cgaacagttc ctgattaacc acaaaccgtt
    13441 ctactttact ggctttggtc gtcatgaaga tgctgacttg cgtggcaaag gattcgataa
    13501 cgtgctgatg gtgcacgacc acgcattaat ggactggatt ggggccaact cctaccgtac
    13561 ctcgcattac ccttacgctg aagagatgct cgactgggca gatgaacatg gcatcgtggt
    13621 gattgatgaa actgctgctg tcggctttaa cctctcttta ggcattggtt tcgaagcggg
    13681 caacaagccg aaagaactgt acagcgaaga ggcagtcaac ggggaaactc agcaggcgca
    13741 cttacaggcg attaaagagc tgatagcgcg tgacaaaaac cacccaagcg tggtgatgtg
    13801 gagtattgcc aacgaaccgg atacccgtcc gcaaggtgca cgagaatatt tcgcgccact
    13861 ggcggaagca acgcgtaaac tcgacccgac gcgtccgatc acctgcgtca atgtaatgtt
    13921 ctgcgacgct cacaccgata ccatcagcga tctctttgat gtgctgtgcc ttaaccgtta
    13981 ttacggttgg tatgtccaaa gcggcgattt ggaaacggca gagaaggtac tggaaaaaga
    14041 acttctggcc tggcaggaga aactgcatca gccgattatc atcaccgaat acggcgtgga
    14101 tacgttagcc gggctgcact caatgtacac cgacatgtgg agtgaagagt atcagtgtgc
    14161 atggctggat atgtatcacc gcgtctttga tcgcgtcagc gccgtcgtcg gtgaacaggt
    14221 atggaatttc gccgattttg cgacctcgca aggcatattg cgcgttggcg gtaacaagaa
    14281 agggatcttc actcgcgacc gcaaaccgaa gtcggcggct tttctgctgc aaaaacgctg
    14341 gactggcatg aacttcggtg aaaaaccgca gcagggaggc aaacaatgaa tcaacaactc
    14401 tcctggcgca ccatcgtcgg ctacagcctc ggtgacgtcg ccaataactt cgccttcgca
    14461 atgggggcgc tcttcctatt gagttactac accgacgtcg ctggcgtcgg tgccgctgcg
    14521 gcgggcacca tgctgttact ggtgcgggta ttcgatgcct tcgccgacgt ctttgccgga
    14581 cgagtggtgg acagtgtgaa tacccgctgg ggaaaattcc gcccgttttt actcttcggt
    14641 actgcgccgt taatgatctt cagcgtgctg gtattctggg taccgaccga ctggagccat
    14701 agcagcaaag tagtgtatgc atatttgacc tacatgggcc tcgggctttg ctacagcctg
    14761 gtgaatattc cttatggttc acttgctacc gcgatgaccc aacaaccaca atcccgcgcc
    14821 cgtctgggcg cggctcgcgg gattgccgct tcattaacct ttgtctgcct ggcatttctg
    14881 ataggaccga gcattaagaa ctccagcccg gaagagatgg tgtcggtata ccatttctgg
    14941 acgattgtgc tggcgattgc cggaatggtg ctttacttca tctgcttcaa atcgacgcgt
    15001 gagaatgtgg tacgtatcgt ggcgcagccg tcattgaaga tcagtctgca aaccctgaaa
    15061 cggaatcgcc cgctgtttat gttgtgcatc ggtgcgctgt gtgtgctgat ttcgaccttc
    15121 gcggtcagcg cctcgtcgtt gttctacgtg cgctatgtgt taaatgatac cgggctgttc
    15181 actgtgctgg tactggtgca aaacctggtt ggtactgtgg catcggcacc gctggtgccg
    15241 gggatggtcg cgaggatcgg taaaaagaat accttcctga ttggcgcttt gctgggaacc
    15301 tgcggttatc tgctattctt ctgggtttcc gtctggtcac tgccggtggc gttggttgcg
    15361 ttggccatcg cttcaattgg tcagggcgtt actatgactg tgatgtgggc actggaagct
    15421 gataccgtag aatacggtga atacctgacc ggcgtgcgaa ttgaagggct cacctattca
    15481 ctattctcat ttacccgtaa atgcggtcag gcaatcggag gttcaattcc tgcctttatt
    15541 ttgggattaa gcggatatat cgccaatcag gtgcaaacgc cggaagttat tatgggcatc
    15601 cgcacatcaa ttgccttagt accttgcgga tttatgctac tggcattcgt tattatctgg
    15661 ttttatccgc tcacggataa aaaattcaaa gaaatcgtgg ttgaaattga taatcgtaaa
    15721 aaagtgcagc agcaattaat cagcgatatc actaattaat attcaataaa aataatcaga
    15781 acatcaaagg tgcaactatg agaaaaatag tggccatggc cgttatttgc ctgacggctg
    15841 cctctggcct tacctctgct tatgcggcgc aactggctga cgatgaagcg ggactacgca
    15901 tcagactgaa aaacgaattg cgcagggcgg ataagcccag tgctggcgcg ggaagagata
    15961 tttacgcatg ggtacaggga ggattgctcg atttcaatag tggttattat tccaatatta
    16021 ttggcgttga aggcggggcg tattatgttt ataaattagg tgctcgtgct gatatgagta
    16081 cccggtggta tcttgatggt gataaaagtt ttggctttgc cctgggggca gtaaaaataa
    16141 aacccagtga aaatagcctg cttaaattag gtcgcttcgg gacggattat agttatggta
    16201 gcttacctta tcgtattccg ttaatggctg gcagttcgca acgtacatta ccgacagttt
    16261 ctgaaggagc attaggttat tgggctttaa caccaaatat tgatctgtgg ggaatgtggc
    16321 gttcacgagt atttttatgg actgattcaa caaccggtat tcgtgatgaa ggggtgtata
    16381 acagccagac gggaaaatac gataaacatc gcgcacgttc ttttttagcc gccagttggc
    16441 atgatgatac cagtcgctat tctctggggg catcggtaca gaaagatgtt tccaatcaga
    16501 tacaaagtat tctcgagaaa agcataccgc tcgacccgaa ttatacgttg aaaggggagt
    16561 tgctcggctt ttacgcgcag ctcgaaggtt taagtcgtaa taccagccag cccaatgaaa
    16621 cggcgttggt tagtggacaa gtgacctgga atgcgccgtg gggaagcgta tttggcagtg
    16681 gtggttattt gcgccatgca atgaatggtg ccgtggtgga taccgacatt ggctatccct
    16741 tttcattaag tcttgatcgt aaccgtgaag gaatgcagtc ctggcaattg ggcgtcaact
    16801 atcgtttaac gccgcaattt acactgacat ttgcaccgat tgtgactcgc ggctatgaat
    16861 ccagtaaacg agatgtgcgg attgaaggcg cgggtatctt aggtggtatg aactatcggg
    16921 tcagcgaagg gccgttacaa gggatgaatt tctttcttgc tgccgataaa gggcgggaaa
    16981 agcgcgatgg cagtacgctg ggcgatcgcc tgaattactg ggatgtgaaa atgagtattc
    17041 agtatgactt tatgctgaag taaaaaataa cgccggagag aaaaatctcc ggcgttttgt
    17101 ctgtgaatta ctgctgcgga atagcgggta cgaccggaac atttaatgcc ggcattgcaa
    17161 acataccaac gaaatcttcc agtggcattt tctgcccgtt taacgttatc tgaccgttag
    17221 tatattgcag gctggtggtg atggtattgt cctgcaaggt ggtcagacgg aacatctgcc
    17281 ccattgctga tgcaccttca acttgctgtt tcgccagttt tttcgcttga tcttcctgat
    17341 aaccttccag cttcgctacc tgagtcataa actcagttgc catatccacc ggaatggtca
    17401 gtttcgcatc cagagattta accgaacgat ctacttcctg cgccagcgtt tgcggcgctt
    17461 ctttagtcgt tgccggatct ttcaggaaca gcgacagatt cagcgcactt tcaccctgac
    17521 tgtttttcca gcttagcggc gcgatagtaa tcaccggatc gcctttcaac atcagcggca
    17581 gggcgctaaa gaaggcttcc gtcactttct cctgataaag ttcgggattg ttggcaattt
    17641 ctggctgtgc cagcagcgcc tgagtttgcg cgttatattg ctggctaaac tgatgccagg
    17701 cttcaccatc aatctggccg acttttaaag tcagcttgcc gctgcccaga tcctgattct
    17761 gtaccttcag gctgtttagc gagtaatcca gttggctatt gatcgtttta ccgtcattga
    17821 ccagatccga tttaccgctg atctccatgc cttccagcag tgccagttct ttgccttcca
    17881 ccgaaatggt cattttttcc agtgacagtt tttggtttcc tacgcgctca ccaaaacttg
    17941 ccagcgtgct ggaaccgtcg gttttcagat tattaaaggt caactgcact ttctggttgt
    18001 attcgttaac tgcgtctatc cgaccacttt gcgcctcccc ggaaagggag atagctttgc
    18061 cgtctctgtc cgcatttaac tggaactcgc cgccgctaaa ggcgactttt tcatcctttt
    18121 gctcgtaatt cagtggcttg agcgaaatat cggaactgga atcaccgctg taaccaatgc
    18181 gcgagttaat ctcaaaaggc gtttcacctt ttgccatatc aaacagtggt ttgcttactt
    18241 cgttattaac cagcgtggtt tgaattgatg ccatcgacgg gatcaggttc agttttttaa
    18301 gctgggcaag cgggaaggga ccatgatcaa ccgattcgtt gaagatgacg ctctgaccgc
    18361 ttttaatcca cggattttct ttcccggcaa tgggtttcac caacagttgc aactggctgc
    18421 tgaatacgcc gcgatgatag ttttgataac tcacttccag gttggattca ggagcggtca
    18481 gtttgagttg cgcgttcgcc tgcgcgacca tgtcttcgag atgggtttca atcttcttgc
    18541 ctgtatacca tgcgccgcct gtccagacta cgcctagcgc aacaatgacg cctaccgcta
    18601 ccagcgattt attcataatg attatccata aaatgaaatc aggcggactg gccgcctgaa
    18661 ggtgttataa gcctttaata agcttagcaa gagatgttaa ttttttcagt aagctcttac
    18721 agcttgttgt aaacacgcgc taaacggccg tggcctttga cagtcaccgg tgattcgttg
    18781 gcggcaataa acgctgattc acccggttta agctgtaact gctgagaacc tttccacaac
    18841 gttgcatcgc cttcgacgca gaacaaaatg gcggcactct gctggctaat ggtggtttct
    18901 ttatcactaa ggtcatgcag cgagaaggca aaatcatcca ctggaatcgg gaagtccagt
    18961 tctgcacctt gtttcaccgg ctgggtcaac aactggttag ccggtttggc ttcgaatttc
    19021 acattggcaa ccagttccgg aatatcaatg tacttaggcg tcagacccgc acgcagcacg
    19081 ttatcggagt tcgccatcac ttccagcgcc acgccttgca ggtaagcgtg cggtgtttca
    19141 gcgaacagga acatcgcttc gccaggattc aatttcacca cattcagtaa cagcggggag
    19201 aacaggccgc tgtcttccgg gtaaaattca gaaattaaac gaatcgtttg ccacggttca
    19261 ccctgctgac tgtctagcgc cgattttaaa atcgccagcg cgcgggattt ttcttcaccc
    19321 tgcatattca acaggctggc gaacagttcg cttaaacgtt cggcattagg ctgttgtaaa
    19381 aagtgagcaa ttgccggatg tgcacctgcg actggctgga gtagggagac aatctcggaa
    19441 aactcacgaa acgcgttcat cgcaaggaaa ggcgtcagcg caaaaaccag ctccggcttg
    19501 tggttaggat ctttatagtt acgctcggcg gcatccatcg ggatacctgc ggcattttct
    19561 ttggcaaaac cgatttcaga attgcgtttg tttggatgaa cctgaatgga gagcggctgt
    19621 gctgcgcata atactttgaa caggaaaggc agttcgccaa agcgtttggc aacggcctct
    19681 ccgagcagag tcgatttatt actctcaatc acatcacgca gtgaaacgat atctccggcg
    19741 gcattctgca cccgtgaact gcttttcgga tgtgcgccca tccacagctc ggccatcggc
    19801 tggctggacg gattttccat accataaagt tcagtcaacg ccgttttgct gccccaggca
    19861 tagttttgca ctgagttaat gagtttttgc atgatcaatc cctgttttaa tgtggaaatt
    19921 aatcccgcta ttaaagcaag aatcctacgg gaagtaacct ggagccgcaa aaagtcgtac
    19981 tagtctcagt ttttgttaaa aaagtgtgta ggatattgtt actcgctttt aacagggcaa
    20041 cggaacaccc gcccagagca taaccaaacc aggcagtaag tgagagaaca atgtcaaaca
    20101 aaccctttca ttatcaggct ccttttccac tcaaaaaaga tgatactgag tattacctgc
    20161 taaccagcga acacgttagc gtatctgaat ttgaagggca ggagattttg aaagtcgcac
    20221 ccgaagcgtt aactctgttg gcgcgtcagg catttcatga tgcgtcattt atgctgcgtc
    20281 cggctcacca acaacaggtg gccgacattc tgcgtgaccc ggaagccagc gaaaatgata
    20341 aatatgtggc gctgcaattc ctgcgcaact ccgacatcgc ggcgaaaggt gttctgccaa
    20401 cctgtcagga taccggcact gcgattattg ttggtaaaaa agggcagcgt gtatggaccg
    20461 gtggctgtga tgaagcggcg ctggcgcgcg gtgtctataa cacttatatc gaagataatt
    20521 tgcgctactc gcaaaacgcg ccgctggata tgtataaaga ggtgaatacc ggcaccaatc
    20581 tgccagcgca gatcgatctt tatgccgttg atggcgacga gtacaaattc ctctgtatcg
    20641 ccaaaggtgg tggttcggca aacaagacgt atctctatca ggaaaccaaa gcgttactaa
    20701 cgccggggaa actgaaaaat tacctggttg agaagatgcg cacgctgggt acggcggcct
    20761 gtcctccgta tcatattgcg ttcgtaattg gtggaacttc tgcagaaact aaccttaaaa
    20821 cagtgaaact ggcttccgct aaatactatg acgaactgcc aacggaaggg aatgagcacg
    20881 gtcaggcatt ccgcgatgtg gaactggaaa aagaattgct gatcgaagcg caaaatctcg
    20941 gtctgggtgc gcagtttggt ggtaaatact tcgctcacga catacgcgtg attcgcctgc
    21001 cacgtcacgg cgcatcctgt ccggttggta tgggtgtttc ctgctctgct gaccgtaata
    21061 tcaaagcgaa gatcaaccgt caggggatct ggatcgaaaa actggaacat aatccaggta
    21121 aatatatccc tgaagagctg cgtaaagcgg gagaaggcga agcggtgcgc gttgacctta
    21181 accgtccgat gaaagagatc ctcgcacagt tgtcgcagta tcccgtttct acacgcttat
    21241 cgcttaacgg cacgattatc gtcggtcgtg atattgctca cgccaaactg aaagagcgga
    21301 tggataacgg tgaagggctg ccgcagtaca ttaaagatca tccgatttac tacgcgggtc
    21361 cggccaaaac gccggaaggt tatgcctccg gttctcttgg cccaacgacc gccggacgga
    21421 tggattctta tgtcgatcaa ctgcaagcgc agggcggaag tatgatcatg ctggcgaaag
    21481 gcaaccgcag ccagcaggtg acggatgcct gtaaaaaaca cggcggcttc taccttggca
    21541 gtatcggtgg tccggccgct gtattggcgc agggaagtat taagagcctg gaatgtgttg
    21601 aatatccgga actgggaatg gaagccatct ggaaaattga agtggaagat ttcccggcgt
    21661 ttatccttgt ggatgataaa ggaaatgact tcttccagca gatacaactc acacagtgca
    21721 ctcgctgtgt gaaataaaca gagccgccct tcggggcggt ttttttacat ggcacgaaag
    21781 accaaacatt tgttatcaaa tagtaaataa taagcgagct aaaagttgct taacgaaagc
    21841 aaaacagaaa gaaaaaatta aacaggtgag gagcaggcca tgaatacagt acgcagcgaa
    21901 aaagattcga tgggggcgat tgatgtcccg gcagataagc tgtggggcgc acaaactcaa
    21961 cgctcgctgg agcatttccg catttcgacg gagaaaatgc ccacctcact gattcatgcg
    22021 ctggcgctaa ccaagcgcgc agcggcaaaa gttaatgaag atttaggctt gttgtctgaa
    22081 gagaaagcga gcgccattcg gcaggcagcg gatgaagtac tggcaggaca gcatgacgac
    22141 gaattcccgc tggctatctg gcagaccggc tccggcacgc aaagtaacat gaacatgaac
    22201 gaagtgctgg ctaaccgggc cagtgaatta ctcggcggcg tgcgcgggat ggaacgtaaa
    22261 gttcacccta acgacgacgt gaacaaaagc caaagttcca acgatgtctt tccgacggcg
    22321 atgcacgttg cggcgctgct ggcgctgcgc aagcaactca ttccgcagct taaaaccctg
    22381 acacagacgc tgagtgaaaa atcccgtgca tttgccgata tcgtcaaaat cggtcgaacc
    22441 cacttgcagg acgccacgcc gctaacacta gggcaggaga tttccggctg ggtagcgatg
    22501 ctcgagcata atctcaaaca tatcgaatac agcctgcctc acgtagcgga actggctctt
    22561 ggcggtacag cggtgggtac tggactaaat acccatccgg agtatgcgcg tcgcgtagca
    22621 gatgaactgg cagtcattac ctgtgcaccg tttgttaccg cgccgaacaa atttgaagcg
    22681 cttgcgacct gtgatgccct ggttcaggcg cacggcgcgt tgaaagggtt ggctgcgtca
    22741 ctgatgaaaa tcgccaatga tgtccgctgg ctggcctctg gcccgcgctg cggaattggt
    22801 gaaatctcaa tcccggaaaa tgagccgggc agctcaatca tgccggggaa agtgaatcca
    22861 acacagtgtg aggcattaac catgctctgc tgtcaggtga tggggaacga cgtggcgatc
    22921 aacatggggg gcgcttccgg taactttgaa ctgaacgtct tccgtccaat ggtgatccac
    22981 aatttcctgc aatcggtgcg cttgctggca gatggcatgg aaagttttaa caaacactgc
    23041 gcagtgggta ttgaaccgaa tcgtgagcga atcaatcaat tactcaatga atcgctgatg
    23101 ctggtgactg cgcttaacac ccacattggt tatgacaaag ccgcggagat cgccaaaaaa
    23161 gcgcataaag aagggctgac cttaaaagct gcggcccttg cgctggggta tcttagcgaa
    23221 gccgagtttg acagctgggt acggccagaa cagatggtcg gcagtatgaa agccgggcgt
    23281 taatctgcaa catacaggtg cagccgtgga atgatcaaac gaagcggctg cgcctgtggt
    23341 ttataacggt gctgcacatt gtcggcatcg taatttaaca actcaccaac gtccggcacg
    23401 cccgcgccat tatcccgatt aatcagtgca atcagtggtg tagggcaggc gtgttggact
    23461 tgtttttgat ctcctttgta ccagacgcgg gcaatcggct gcaccttcac cggacgtttg
    23521 atttttaact tcgcgttctg tggcagggca gcgatatcct gatactctcg ctccagtttt
    23581 ctttgccact cctcgcgcgt ccacggtgcg acactgcgtg gtgatttcag gcttttttcc
    23641 agctgtgcca ggacttcatc acgatgcaaa ttcttaatga tatgtttatt agcccaacca
    23701 aagcgtaaag tggcggggtc gtgcagaacg gtgagcgtgc ggtaagcatt aagggtgatc
    23761 agccccggca aatgacgatg cacccattca aaacgtgccg cggtggggag ttctgattca
    23821 accgtgacga tatgctcgaa cgtggtcttg agtttattga tgtgctgaat atgactgacc
    23881 aacgctgctt gcgaaaggtt atcgacctgg taacacaaca cgccaggcag acggacagcg
    23941 gccttgctgc tgcgattttc ggactgttgt tgaataaata aatggcggaa atgacgcaac
    24001 gccagcgact gcgcgtcgtt gccgagatgt tgttttacct caatacgatt aagcggatta
    24061 tgctcatcct ctttttttac ctccggcaaa gagaacacgc gggcaaccaa tagcttgtgt
    24121 tgctcaagat gagcggcaaa tgcagccagc tcttgttcca tctggcgaaa ggtagtgttg
    24181 agtcggtcta cgagatcgta acgcgccata atattaacca ctttagttac aacatactta
    24241 ttttatagca cagtcgtggt gacttgacca tcgtgcgcgt agtgtcaggc agaggtaaat
    24301 tgcgggatgt tatgccataa cggccagcta aacgagaagc gagcaccacc cagttcgctg
    24361 gtgtcacaat taaccgtacc gcccattgcc agtgctatag agtggacaat tgccagcccc
    24421 agcccgcagc cgccggttga gcgatcccgg ctgggatcga ggcgaacaaa aggttcaaag
    24481 atatgttcgc ggttttctgg ggcaatccct gggccatcat cctcaacaat taatgtcgct
    24541 ctattccccg acagtagcag gctggtttca accgttgaat ggcagtaccg cagggcgtta
    24601 ttgagcaaat tatccagcac gcgctccatt aagcgcatat ccaacgccgc ataatggcct
    24661 tgcgcgagcg ttttaatccg taccgtttta tcgggcgtta ctgcctgaat gtctgccaga
    24721 tgcgttgaca gccacaacgg caggtctggt tcgctaagat gaagctcgtt ttgtgggcga
    24781 tcgagtcggg cataagtcag cagctcttca attaaagctt caagttgact gatatcacga
    24841 ttcaacgcct gggattcggc ggcgctcagg ttatcgctca tctccagtcg ataacgcagg
    24901 cgcactaacg gtgttcgcag ttcgtgagcg ataccgtcaa taagctgttt tttgctggca
    24961 atcaaggcgt tgatattgtc cgccatctgg ttaaatgcga taccaagtcg ttcaaagctc
    25021 gaaccctcat caaagtggat acgctcattg agatgcccat cgccaaatcg ttgcgccgct
    25081 gcttccagtt ttaacatatc ctgccagtgc ggacgcatcc agataaacac cggaaaggcg
    25141 agggaaatag caataaaagc gatcagggcg atatccagca atcgcatctg atggagataa
    25201 taaagataag gaacaggacc aactgccagc acgtagtggc tgcgcgggat acgctgcaaa
    25261 aacgtgtact gatcgtccag ggcgacaatt tcgccgccac gcagtcggtg catggaaata
    25321 tcatcaagat ggtatttact cagtggctcg acacgcagat cgaaagagag atttaaatcc
    25381 atctctttca gcgtcttacc ccagtcgtgt ggggggatct cacgcaattc gctgcgcatc
    25441 agatacagcg aactgttcat caaatcatcc agcgactgtt tgcccgcgcg ttcggcggta
    25501 aatttgtaca ccagcccaac cagcagagac atcacaagga agcagacaaa caataacagg
    25561 taaaactgga taaacagttt tttcatcgct tattcccatg catgaggcgc aaaaagatag
    25621 cctttgttac gcacagtttt aatgcgataa ggttctgcgg cgttatcgag cagttttttt
    25681 cttaaccgcg aaatagccac gtccacgcta cgatccagtc cgtcataact gacgccgcgt
    25741 aaatttttca gcaatgcatc gcggtccatg atttgcccgg catgggtagc taattcccac
    25801 aataattcga aatcagctgt cgagagcgag atttcagtgt tagccagggt gactacgcgg
    25861 ttgatgggat cgatggtcaa cgtgccgaaa tgcagggctt tgtagggagt cagagacgtt
    25921 tcctgaagac ctttggtcag tgtggcttgc tcattctgac gcaaatgcaa acgtaaacgc
    25981 gctagcaaaa cagcaggggg cgtcgttttg agaatatagt cgcaggcacc catttccagt
    26041 gccaggatgt ggttcatatc gctatcgaga gaggttagaa gaacaatcgg tccagaccac
    26101 tttgcgcgta aatcacgaca aatggtcatg ccgtccttgc ctggtagcat gatgtcgagt
    26161 aacaccaaat ccggactttc tcgcaaaatg gtttcttcgg cctggtcgcc gcgcggttct
    26221 acggtaacct gcatatcatg ttttgccagg tacgcggcaa tcagtgaacc gacttccgca
    26281 tcatcttcca caaatacgat agtgttcata acattcacgg tagatataaa aacgtcacaa
    26341 tacaccgcgc tgtttttact tactatcaat cgtttctaaa caagttttca tcaggtattc
    26401 tactgatgac ttgttatgga agtgttaagg taaaaagatg gggctggtaa tcaaagccgc
    26461 gctcggtgcg ctggtagtgc tgttgattgg tgttttagca aaaacgaaaa attattatat
    26521 cgccgggctg attccacttt tcccgacctt tgcgcttatc gcgcattata ttgttgccag
    26581 cgaacgcggc attgaagcct tacgcgcaac catcattttt agtatgtggt cgattattcc
    26641 ctattttgtt tacctggtgt cgctgtggta tttcaccgga atgatgcgct tgcccgccgc
    26701 ttttgttggt tctgttgcgt gttgggggat aagcgcatgg gtgttgatta tatgctggat
    26761 aaagctgcat taacgcagat gacgaccgcc atcaagtggg aaactgcgtc cggtgacaaa
    26821 gcagctggta agtaagtaat cgaccaggtc gatcacttct ttctcgccag gcgcggtttt
    26881 catcagtgat ttattcagcg cctgttgtcg atattcggca tcatcatgtt cattaaacag
    26941 gatcagcgat ggcgcaatag aattcacttt cacttccggt gccagcttgc gggcaaacga
    27001 gcgggtcata ttatccagtg ccgctttgct tgcagcatac gcaacatgtt tgtcgctacc
    27061 gcgctccacc acataatcgg taaagtgaat gatatcgctg gcggcgtgtc cgtgcccacg
    27121 cagtaatctt tccagcgcat ggttgagcag gtatggggta ttaacgtgga tctgcatcat
    27181 gcaagccagt acgtcggcca gtggtgtacc tggtttttcc gccatccacg cactggcgtt
    27241 atgcaaaata gcacgcagac catgggtgct ttttagtact tcatcggcaa acgccatcac
    27301 accgtcgttg gtcgaaaaat cagcctgaat acactgcgca cctgccttaa tcagtccatc
    27361 aatggctgga tagtgcgtcc gatagctgac aatcaccggt tgcttttgat taatgaaatg
    27421 ccaggcgagg gcgaggccga tgcgacgacc tccgccagta attaatattg gcaagggctg
    27481 ggctttaccc atcgttatct cctttgctat ccaacgatgg gatgaacgtt atcccaccag
    27541 catccaggtc gccggaactg aggcaattaa cagcatgcca atcagcacca tttcctgacg
    27601 gttcagtaca ttatcatgtg tatgcgtttt gcgcgcatac aggaaaacca gcagccccgg
    27661 cgcatacaga acaacggata acagcaagtg cattggacca gaggcataca ataaccataa
    27721 gccataaatg caggcaccga cacctaccgc tttatgcagt ggacgggttg cgattttcag
    27781 caagaacgcg ccgaccagga aatagggcac cagaatcatt tctgaggcga tggtcagcaa
    27841 cgtgttgtaa tcggaaccgg tcagccagat gagtaccaga caaatttgca cgcagatgtt
    27901 ggtcaaccac agcgaggcgg atggcgcagc ttgtgcgttc tggcgcgcaa aaatgcgtgg
    27961 gaatgcttta tgagtggcag ccaggaacgg aacttctgcc gccatgatgg tccagcttaa
    28021 gtacgcgccg caaacggaaa cgatcaaacc ggcagcgatg atgatttcgc cccatggtcc
    28081 catcatttcc accatcagac cggccattga cgggttacga atttcagcca gttcaggacg
    28141 tgccaccacg cccagcgaaa gcagcgttac cagcaagtaa acgcccagag cggagagaac
    28201 cgccagcagt gtcgctttgc caacatcacg tttattacgc gcacgcgcag aaacaaccac
    28261 ggcaccttcc acaccaatga aaacccacag ggtgatcagc atggtgtttt tcacctgttc
    28321 ccacacgggt acgccaagtg caagtccggt gaagtcgagc ttgaaggtat ccagtttgaa
    28381 catcatcatc gccagcacaa caaacagacc cagcggcaac aattttgcca gtgtcgccac
    28441 caggttaatg ctggcagcgg tttgcacccc gcgcaggatc aaaaagtgaa caatccacaa
    28501 taaagccgat gcaccgacta tcgattgcca ggtattacca tcgccgaaca ggcgcaattc
    28561 cggcgtgtcc gtaaaaaagc ttaacgcgga aaaaacgatc accagatagg agacgttggc
    28621 gatgactgcg cacagccagt atccccatgc ggaacaaaag ccgattagct cgccaaaccc
    28681 ttcgcgggca taggtaaaga taccgccgtc aagttcggga cgaatgcgcg tgaggatcag
    28741 catggcaaag gccagcaata aaatgccagc gccagtaata ccccagccga tgagcagtgc
    28801 tgccgggctg gcaactgccg ccatattttg cggcagactg aaaacacccg cgcccagcat
    28861 tgagcttaat accagcgcgg tgagtgcgct cagtcccagt ttcttttcca tcgatatcca
    28921 gtgattaaaa cgtgataaga agaataaata ttagggcaaa acgcataaaa atggagtttg
    28981 ccactgaagc gcgggatttt acgaagagat gtgtctgcat gcaatggtcg cggggaaatt
    29041 tgtgactaaa aatgcgaaag gcggcatcaa gccgccttat aggagtaact gaatagttat
    29101 ttatacagat ctgcgctgac ggtcaggtta ttaccacgtt cctgccactg gcgggtgatg
    29161 tggtaatact tagcaccttt cttcgcggca cgtttcgcaa cctgatagga gacttcggtc
    29221 atgttgccgt agttgccaga gaatttgatg ctgtcgaacg ggaccatcat cgctgctgtc
    29281 gctttgttca gttcttcgac tttagtgccg tcaggaagcg tgacagtgta acgtccgcct
    29341 tttgatgact gggtttcaaa gaaacgaccg acttcagaac ttggtgatgc ggtagtcgca
    29401 acacctggga tctcaacttt cttcgcggct tcgccaccgg cagccagagc tgcacgtcct
    29461 gcttcggaat ctgccgggat cacatccggg ctctggacga tacgtttctt agcatctttt
    29521 ttatagatga atgcagtaat acgctggttg ccgccctggt tggcatcgat ttgacgaaca
    29581 atgtagaaag agtaggcacc tttcgctttt gctgctttgg tgatggcatc attgacttct
    29641 ggctggctac gatagaagcc ctggacggtg accgtgtcaa acggttcaat cagaacagcc
    29701 tgatctttcg gcagttcgac aacaccgtta attacgcgat tacttgtttc ttctgctttt
    29761 tcagcatcgg ctttatagag gtcagcgacc acacgccagt taccgctgtt accaaaatca
    29821 gaagtgtcga caacataaaa agaggcggca ccttctttat ctgcgcgacg agaaacggct
    29881 ttcaccgctt cgccaatagc attaaaacga ccggtaacca ctacacggtc aaaaggttta
    29941 accgctgccg cttgctccgg tgtcagttct gttgctgcgt taacggagaa tgccgtagca
    30001 gaaagcagtg ccgacgccag gagggtgttc ttaagcttca taaaaataat ccttcgcctt
    30061 gcgcaaacca ggtactggta ttgttattaa cgagaaacgt ggctgattat tgcatttaaa
    30121 cggtgtaact gtctgcgtca tttttcatat cacattcctt aagccaattt taatcctgct
    30181 caaatgaccg tctatgctta aaaaacagcc gtatcagcat cattactact gaagcaactg
    30241 aattgtataa gttaatttaa tgttaagtag tgattcgtgc cggggcgatg tctcgtttta
    30301 cccgaccgtc gaagacaatt atcagtcttt atccggcgtt ctaaggtgtt tatcccacta
    30361 tcacggctga atcgttaata ttttgcgagt tcacgccgaa atactgattt ttggcgctag
    30421 atcacaggca taattttcag tacgttatag ggcgtttgtt actaatttat tttaacggag
    30481 taacatttag ctcgtacatg agcggcttgt gtggctcctg acacaggcaa accatcatca
    30541 ataaaaccga tggaagggaa tatcatgcga attggcatac caagagaacg gttaaccaat
    30601 gaaacccgtg ttgcagcaac gccaaaaaca gtggaacagc tgctgaaact gggttttacc
    30661 gtcgcggtag agagcggcgc gggtcaactg gcaagttttg acgataaagc gtttgtgcaa
    30721 gcgggcgctg aaattgtaga agggaatagc gtctggcagt cagagatcat tctgaaggtc
    30781 aatgcgccgt tagatgatga aattgcgtta ctgaatcctg ggacaacgct ggtgagtttt
    30841 atctggcctg cgcagaatcc ggaattaatg caaaaacttg cggaacgtaa cgtgaccgtg
    30901 atggcgatgg actctgtgcc gcgtatctca cgcgcacaat cgctggacgc actaagctcg
    30961 atggcgaaca tcgccggtta tcgcgccatt gttgaagcgg cacatgaatt tgggcgcttc
    31021 tttaccgggc aaattactgc ggccgggaaa gtgccaccgg caaaagtgat ggtgattggt
    31081 gcgggtgttg caggtctggc cgccattggc gcagcaaaca gtctcggcgc gattgtgcgt
    31141 gcattcgaca cccgcccgga agtgaaagaa caagttcaaa gtatgggcgc ggaattcctc
    31201 gagctggatt ttaaagagga agcgggcagc ggcgatggct atgccaaagt gatgtcggac
    31261 gcgttcatca aagcggaaat ggaactcttt gccgcccagg caaaagaggt cgatatcatt
    31321 gtcaccaccg cgcttattcc aggcaaacca gcgccgaagc taattacccg tgaaatggtt
    31381 gactccatga aggcgggcag tgtgattgtc gacctggcag cccaaaacgg tggcaactgt
    31441 gaatacaccg tgccgggtga aatcttcact acggaaaatg gtgtcaaagt gattggttat
    31501 accgatcttc cgggccgtct gcctacgcaa tcctcacagc tttacggtac taacctcgtt
    31561 aatctgctga aactgttgtg caaagagaaa gacggcaata tcactgttga ttttgatgat
    31621 gtggtgattc gcggcgtgac cgtgatccgt gcgggcgaaa ttacctggcc ggcaccgccg
    31681 attcaggtat cagctcagcc gcaggcggca caaaaagcgg caccggaagt gaaaactgag
    31741 gaaaaatgtg cctgctcacc gtggcgtaaa tacgcattga tggcgctggc aatcatcctt
    31801 ttcggttggc tggcaagcgt tgcgccgaaa gagtttcttg ggcacttcac cgttttcgcg
    31861 ctggcctgcg ttgtcggtta ttacgtggtg tggaatgtat cgcatgcgct gcatacaccg
    31921 ttgatgtcgg tcaccaacgc gatttcaggg attattgttg tcggagcact gttgcagatt
    31981 ggccagggcg gctgggttag cttccttagt tttatcgcgg tgcttatagc cagcattaat
    32041 attttcggtg gcttcaccgt gactcagcgc atgctgaaaa tgttccgcaa aaattaaggg
    32101 gtaacatatg tctggaggat tagttacagc tgcatacatt gttgccgcga tcctgtttat
    32161 cttcagtctg gccggtcttt cgaaacatga aacgtctcgc cagggtaaca acttcggtat
    32221 cgccgggatg gcgattgcgt taatcgcaac catttttgga ccggatacgg gtaatgttgg
    32281 ctggatcttg ctggcgatgg tcattggtgg ggcaattggt atccgtctgg cgaagaaagt
    32341 tgaaatgacc gaaatgccag aactggtggc gatcctgcat agcttcgtgg gtctggcggc
    32401 agtgctggtt ggctttaaca gttatctgca tcatgacgcg ggaatggcac cgattctggt
    32461 caatattcac ctgacggaag tgttcctcgg tatcttcatc ggggcggtaa cgttcacggg
    32521 ttcggtggtg gcgtttggca aactgtgtgg caagatttcg tctaaaccgt tgatgctgcc
    32581 aaaccgtcac aaaatgaacc tggcggctct ggtcgtttcc ttcctgctgc tgattgtatt
    32641 tgttcgcacg gatagcgtcg gcctgcaagt gctggcattg ctgataatga ccgccattgc
    32701 gctggtattc ggctggcatt tagtcgcctc catcggtggt gcagatatgc cagtggtggt
    32761 gtcgatgctg aactcgtact ccggctgggc ggctgcggct gcgggcttta tgctcagcaa
    32821 cgacctgctg attgtgaccg gtgcgctggt cggttcttcg ggcgctatcc tttcttacat
    32881 tatgtgtaag gcgatgaacc gttcctttat cagcgttatt gcgggtggtt tcggcaccga
    32941 cggctcttct actggcgatg atcaggaagt gggcgagcac cgcgaaatca ccgcagaaga
    33001 gacagcggaa ctgctgaaaa actcccattc agtgatcatt actccggggt acggcatggc
    33061 agtcgcgcag gcgcaatatc ctgtcgctga aattaccgag aaactgcgcg ctcgtggtat
    33121 caacgtgcgt ttcggtatcc acccggttgc ggggcgtttg cctggacata tgaacgtatt
    33181 gctggctgaa gcaaaagtac cgtatgacat cgtgctggaa atggacgaga tcaacgatga
    33241 ctttgctgat accgataccg tactggtgat tggtgctaac gatacggtta acccggcggc
    33301 gcaggatgat ccgaagagtc ctattgctgg tatgcctgtg ctggaagtgt ggaaagcgca
    33361 gaacgtgatt gtctttaaac gttcgatgaa cactggctat gctggtgtgc aaaacccgct
    33421 gttcttcaag gaaaacaccc acatgctgtt tggtgacgcc aaagccagcg tggatgcaat
    33481 cctgaaagct ctgtaaccct gacggcctct gcggaggccg tcactcttta ttgagatcgc
    33541 ttaacagaac ggcgatgctt tgacctcccg ctgtttgttc aagcgcaatt ttgacaataa
    33601 ttgttaacgg cacggaaagc agcataccca ccggtcctaa caaccatccc caaaaaatca
    33661 acgacaaaaa taccaccaat gtggaaagcc ccagcccacg tcccatgatg cgcggctcca
    33721 gaatattgcc gaagaccaga ttaatcagca gatatcccgc cagcaccagc aacgcttcgt
    33781 agaagccatt aaacaccagt acctgagcga taggggggat tgccgcgaga actgaaccaa
    33841 tattagggat gtaattaagc gcaaaggcca gcaatcccca gacaaaagcg aagcgaacat
    33901 cgagtgcggc gagcatcgcc caggcgacca ggccggtgat gatgctgatg gctgttttca
    33961 gcaccagata atgagaaaca ctgtcaattg cacgttgaat cgccgccatc ccttcaaccg
    34021 gacgcgccat catttgctga aattttccgg gcaattgtgg cacttcgagc agcataaaca
    34081 gcaccgtcag cagcaataaa aatattgatg acatggcatt agataactgc gtcaataagt
    34141 tggtgagcaa cgtcatcgcc gcgttcggat caatgtaatg cgccagctgg tcaactgaga
    34201 cgtcaatccc tacgcgttgc aacaacggct caagagcttg cagcggcgtc ataatagagt
    34261 tgcgatattg cggtaacgtc cgcgtcaact cgttgagcgc ggaacccaga taagctaata
    34321 gcaacaccat cgccatcacg atgatggtca tcaaaatcga caccgccagt acacgcggca
    34381 ctcgccagcg gaccatgtgt tgcaccagcg ggttaagaat aacagcaata aataatgcga
    34441 gaataaacgg cacgatgatc tcggcggcaa aacggatacc gcagagaata atgaccagca
    34501 ttcccaacat aatgacgatt tttaggccat tgagcgtgat gatcggcttt gccatgcttg
    34561 tccccgtttt ttcttccttt gctaataata atgtcttttt cgctccgtca tcataacgaa
    34621 aatcaaaccg ctcaggtaaa gaagtgaaaa tattttgagt taattcttaa gctatgatac
    34681 aaatcaggcg tgttcaacta ccgaggacaa ttatcatccg cgatgacgag aagcaacact
    34741 gcggataatt gtaatattat ggacaatttg ttcaggacgt tattttcaac atttacccat
    34801 cttcgtacct catccactat cttgctcgta ggtgagcagc actggcgcaa cgcgctttag
    34861 tcctttcttc tgcctaagct tgccctatgc gcggggcttg accactttac tttggtttcg
    34921 ctgaattaag cgaaaattaa aataattctc ttgcaggaga aggacaatgt atatttattg
    34981 gattttatta ggcctggcta ttgctacaga aattaccggt acgctgtcaa tgaaatgggc
    35041 gagcgtcagt gagggaaatg gcggctttat tttaatgctg gtgatgattt ctctgtcgta
    35101 tatatttctc tctttcgccg ttaaaaagat cgccttaggc gtagcttatg cgctgtggga
    35161 aggtatcggt attttattta ttaccttgtt tagcgttttg ttattcgacg aaagtttatc
    35221 gctgatgaaa attgccgggt taaccaccct ggtcgccggg attgtgttga tcaaatcagg
    35281 tacccgtaaa gcgcgtaaac ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc
    35341 acgccgcctg gctggcattg gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat
    35401 tttctgacgg ctttcgtcgc aaaatatttg gcttgctctc cctggcggcg gtgctggctg
    35461 cctttagtgc gctttctcag gccgtaaaag ggatcgactt gtctgtcgct tatgcattgt
    35521 ggggcgggtt tggtattgcc gccacgttag ccgcaggttg gatcttgttt ggtcaacggt
    35581 taaatcgtaa aggctggatt ggcctggtct tgctgttggc tggaatgatc atggtgaaac
    35641 ttgcctgatg aagacgctgc ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca
    35701 gttgatccag cttgtcgcga aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc
    35761 gatctttcgc ggcaggagcc gaactttgca ccccaattaa ttgccagccg tcatcggtat
    35821 gcaacatcag aggcgaaccg ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg
    35881 acgtttgcgc ccagccagtc acttcacagt tttgatgact gtataacgta tcgagatgat
    35941 cttcaggata tcccgcctga gtcactttac gaccagccga ttttaatgcg gcagtaagcg
    36001 cggctttatc tccctcaaat aacggcaacg gtgtaatgcc agaaggggga ttacgcagca
    36061 caatcaagcc gaagtcccat ggtgcagctg cgggaggaac aatccaacca tccccatctg
    36121 cttttaaccg ctttcccagt gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc
    36181 gccaaagacc tttatttgac acaaaacgca gcgccactgc tttatcggct ttaccctttg
    36241 gaggtgtcaa taaacagtgt cctgccgtta atgccagatt gggtgcaatc agcgtcgccg
    36301 tacacaaatt gccgctggcc gtttccagtt gcccaaccgc atcccacggt gattgggtcg
    36361 tgtcattcac tggcacacga tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg
    36421 ccgatttggc aacgtctggt ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac
    36481 ccaacactac agcaatggtt gtacgcatat cacactctgg tgggtaatta tgattattaa
    36541 aagcaaaccc tcataaatac tatagacggg acgggatgaa agtgggagta aaatcagata
    36601 actacagtca ggaaagataa aaacgggcgg caatcagtgc gcataaaata aggatgataa
    36661 gaatgaactc aaagcgataa cggcccacca tactaccctc cgctaaaggc ggtgctaaac
    36721 gttacccgct tagcaccgta attttcaggc gcgagggggc gcgccagcat tactgttgaa
    36781 aacttacgct gcgggttgtg cagcaggttt tgccggttgc tgatggctgt gtttcccggc
    36841 gtgtttcttc gctgcctgcg ctttttgttc aggggctttc tgttcagctt tcgtattttt
    36901 atgatgcttt ttagccgcct gcgctttctg ggcaggtgct gctttatgct gttttttatg
    36961 atgtgtcgtt ttcgccggcg ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc
    37021 agtctctgca gcaaaggcgg cagaagacag acccatagca gcggcaacaa ccagagctaa
    37081 tacttttttc attgtcatac cctcaatttg ttttttcatt taaccccact gcggggccgt
    37141 tgaaataact atatccctgg gaatggcaga cttccgtgag tggttggttt cagcgtgtaa
    37201 cgatatgtac aaacgctgaa taaattacag cgttgataat aacgtattgt gacttaaggg
    37261 aaatttagct accaataata gtagtcttga tcgggttaac tattttacta cttacaaagc
    37321 ggtagaataa ctgcgcatca gtaataaatg acacacagca aaatgaatcc gtttatttgg
    37381 gtacttataa tcctgatgac gctagacgcg ctgcgggaat tggctggcgc ttcgtctatt
    37441 ttaggatggc tattaacgtt ggtttgagca agcaataagt ccggacgtgt agttaccgca
    37501 gtccgggctt atttttcagg cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc
    37561 aatgaccagc atcagagcaa taaatgcgcc gacgccgttc cagccatagt tatgccagaa
    37621 aacaccaccc agcgtcccgg caatactcga ccccagatag taactgaaca gatacagcga
    37681 ggaggcctgg cctttagcgc gttttgcgcg ggggccgatc cagctgctgg ctactgagtg
    37741 ggctgcgaag aatccggctg agaagagtaa cattccggca aagatcagcc acagcgagtt
    37801 gaataaggtc atcagtaaac caaacagcat aacccccgtc gaaaacaaca tcaccggacc
    37861 gcgcccgtag cgggtggtca tggttccggc tttaggtgag ctccatgtac cggtcaaata
    37921 agccagcgat aataagccaa ccacggcctg actgacatgc cagggggaaa gcatcaaccg
    37981 atagccgatg taattaaaca gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa
    38041 caataacggt aatccccggt cacgccagtg cagacgaaag ttgataaata acgtcttagg
    38101 gcgcagcgaa gtcgggcgaa aatggcgtga ttccggaagg attttccaga acatcaacgc
    38161 cgaggctagc gcgaaacaac cgattgccgc cagagcaatt cgccagttga aaaagtccgt
    38221 gaagacacca ctaattaagc gtccgctcat gccgccaatt gagttgccgc tgatatacaa
    38281 ccccatcgaa aaggccacga aactggggtg gatttcctcg ctaagataag tcatgccaac
    38341 agctgccacg ccacttaatg aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca
    38401 gctggtcatc attgtcgaaa gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat
    38461 cactggtttg cgaccaatgg catcggatag ggggccagta aacagcaaac caatagccaa
    38521 catcgccgtg gaaatggaca gtgaaatact actgttcgcg ggcgttaagc caaactcctg
    38581 cgaaagcacc ggaaggatag gttgcacaca atagagaagt gcgaaggttg ccagtccggc
    38641 agagaacagc gccagggtga cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt
    38701 tggctgagaa atgctttgct tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt
    38761 acggctcact tgaaatcctt gctaaatatg cctgtggatc aggcttatac atagggtagg
    38821 aaaatcgaat tgttctgtct aatatattaa taatctcaaa taagatgttt taaatatgaa
    38881 tattgaactt cgtcatctgc gttactttgt tgctgttgcg gaagagctgc atttcgggcg
    38941 cgccgctgcc cgcctgaata tttcgcaacc accgctaagt cagcagattc aggcactgga
    39001 gcaacaaatt ggtgcccgac tgctggcacg aactaatcgc agtgtattgc tgacggcagc
    39061 aggaaaacag tttcttgcag atagtcggca aatcctgtct atggtagatg acgctgccgc
    39121 tcgcgctgaa agactgcatc agggggaagc gggggagttg cgcattggtt ttacttcgtc
    39181 cgctcctttt attcgggcgg tgtccgatac gttatcgctg tttcgccgtg attatcccga
    39241 tgtccattta caaacccgcg aaatgaacac tcgtgagcaa atcgctccgc tcattgaagg
    39301 aacgctggat atgggattgc tgcgtaacac agcgttaccg gagacgcttg aacacgcagt
    39361 catcgtccat gaaccgctta tggcgatgat cccgcacgat catcccctgg caaataaccc
    39421 gaatgtaacg ctggctgaac tggcgaaaga accctttgtc ttttttgatc cgcacgtcgg
    39481 gacagggctg tatgacgata ttctcggact gatgcgacgt taccatttga cgcccgtcat
    39541 cactcaggag gtgggcgagg caatgaccat catcggtctg gtttccgccg gtctgggtgt
    39601 ttcaattttg cctgcttcat ttaaacgtgt tcagctcaac gaaatgcgct gggtgccgat
    39661 tgctgaagag gatgcggttt ctgaaatgtg gttggtctgg ccgaaacatc atgaacaaag
    39721 tccagctgcg cgtaactttc gtattcatct gctgaatgct ctcaggtgag ggaaatttca
    39781 gcgaaaaagc ccgaaaaatg tgctgttaat cacatgccta agtaaaaatt tgacgacacg
    39841 tattgaagtg cttcaccata gcctacagat tatttcggag cgcgaaaata tagggagtat
    39901 gcggtggttg ctgaaaacca gcctgggcac attgatcaaa taaagcagac caacgcgggc
    39961 gcggtttatc gcctgattga tcagcttggt ccagtctcgc gtatcgatct ttcccgtctg
    40021 gcgcaactgg ctcctgccag tatcactaaa attgtccgtg agatgctcga agcacacctg
    40081 gtgcaagagc tggaaatcaa agaagcgggg aaccgtggcc gtccggcggt ggggctggtg
    40141 gttgaaactg aagcctggca ctatctttct ctgcgcatta gtcgcgggga gattttcctt
    40201 gctctgcgcg atctgagcag caaactggtg gtggaagagt cgcaggaact ggcgttaaaa
    40261 gatgactcgc cattgctgga tcgtatcatt tcccatatcg atcagttttt tatccgccac
    40321 cagaaaaaac ttgagcgtct aacttcgatt gccataaccc tgccgggaat tattgatacg
    40381 gaaaatggca ttgtacatcg catgccgttc tacgaggatg taaaagagat gccgctcggc
    40441 gaggctctgg agcagcatac cggcgttccg gtttatattc agcatgatat cagcgcatgg
    40501 acgatggcag aggccttgtt tggtgcctca cgcggggcgc gcgatgtgat tcaggtggtt
    40561 atcgatcaca acgtgggggc gggcgtcatt accgatggtc atctgctaca cgcaggcagc
    40621 agtagtctcg tggaaatagg ccacacacag gtcgacccgt atgggaaacg ctgttattgc
    40681 gggaatcacg gctgcctcga aaccatcgcc agtgtggaca gtattcttga gctggcacaa
    40741 ctgcgtctta atcaatccat gagctcgatg ttacatggac aaccgttaac cgtggactca
    40801 ttgtgtcagg cggcattgcg cggcgatctg ctggcaaaag acatcattac cggggtgggc
    40861 gcgcatgtag ggcgcattct tgccatcatg gtgaatttat ttaacccaca aaaaatactg
    40921 attggctcac cgttaagtaa agcggcagat atcctcttcc cggtcatctc agacagcatc
    40981 cgtcagcagg cccttcctgc gtatagtcag cacatcagcg ttgagagtac tcagttttct
    41041 aaccagggca cgatggcagg ggctgcactg gtaaaagacg cgatgtataa cggttctttg
    41101 ttgattcgtc tgttgcaggg ttaacatttt ttaactgttc taccaaaatt tgcgctatct
    41161 caatttgggc caggaaagca taacttagac tttcaaggtt aattattttc ctggtttata
    41221 tttgtgaagc ataacggtgg agttagtgat gctgaagcgt ttctttatta ccggtacaga
    41281 cacttctgta gggaaaacgg tggtttcccg cgcattgcta caagcgttag cctcccaggg
    41341 aaaaacggtt gcgggatata aacccgtagc gaaggggagc aaagagacac ccgaagggct
    41401 gcgtaataaa gatgccctgg tgttgcagag tgtttcaacc atcgaactgc cttatgaagc
    41461 agttaatcct atcgcgttaa gcgaagaaga aagtagcgtg gcgcacagtt gcccaatcaa
    41521 ttacaccctc atttcaaacg gcctggcaaa cctgaccgaa aaagtcgatc atgtcgtggt
    41581 agaagggact ggcggctggc gcagtctgat gaatgatttg cgtccactct ctgaatgggt
    41641 agtgcaggaa caactgccgg tgttgatggt tgtcggtatt caggaaggtt gcattaacca
    41701 tgcactgcta acagctcagg cgatcgccaa cgacgggctg ccgctcattg gctgggtggc
    41761 taaccgaatc aacccaggac tggcgcatta tgcggaaatc attgatgtgc tgggtaaaaa
    41821 acttccggca ccgctcattg gtgaactgcc ttatctgccg cgcgccgaac agcgtgaact
    41881 ggggcaatac atccgcttag ctatgctgcg cagtgtgctg gcggtagata gagtcacggt
    41941 gtaacgtccg cgaaattacc gacgcaatta cgcaggcaat caataaaccg gggagtagct
    42001 gatactcccc ggtcatttca catatcatca acgtcgacat aatcggcgcg tgcgtggtcg
    42061 ccgccaacag tgtcgccatc ccggtcaatc cgagtaaaag tgtaatttct tcgccatcag
    42121 ggaaccataa tcccaggcta cgaccataca acatgccaat ggcaagaccg ataaatagcg
    42181 tcggtgtaaa gacaccaccg ggtgcgccgg aaccgctact cgccagcacg gcacacagtt
    42241 tacaaaggaa gatcccggca atgatcatta acagtggtgg ggcggttaaa aaggattgta
    42301 cggtgctata gccgttgccc cacactgcag gtgtgaacag ggaaagcaga cccacgatca
    42361 acccgcctag tgccagttgc cagggcggcg caagtttgag actcacaaat ccacgatgac
    42421 aggcgttcat taacgttaac aacagtggtc cgcacagacc tgccagcaca cctgtactga
    42481 taatcagcgc atagtcacga gcctgaaccg tcactgagag ttgtacgttg tagagtaacg
    42541 cgtcgctatg attaatcaga ttgctaacca gcaatgccac gacggcggaa ataatcaccg
    42601 ggccgagaga ggccaacatc atagtgccaa acagcacttc ggctataaat aaactgccag
    42661 caagcggggc acgataggcc gcagccattc ccgccgcggc cccacaggcg atccataatt
    42721 tccactcctg gcgtggcgta aaacgttggg caaaacagga ggcggcaagg gcagctaaaa
    42781 gaatcatcgc accttcgcga ccaattgcac tgccgctggt tactaccagc agagaggcaa
    42841 gcgatttaac caggcttgct gcgtaatcga actgtccatc ggtttgcaac gcttccatgt
    42901 aatcggtcgg cgcatgaggg cgttgttggg taaatttctg ccagcccatc agcaacaaac
    42961 ccgccgccag tccgccgagc gccggagtta gcaaccgtcg ccagggggaa aggtttgtcg
    43021 ctgcattgac cagactgccg gagtcattat tgaggaacaa ccactccagt agcagcatcg
    43081 catgacgaaa cccggcaacg gcaaaggccg cgagaatacc gacgactgtt gcgataagca
    43141 gacggcggaa catagtgcgc agatccggat agatgtgtag atgatgcatg agcagaaatg
    43201 ggcaatgaaa cacgggcgac tattttgcgg caaaagaggc cagccgcaaa agcagtgcgt
    43261 gctttaagag ataaaaaaaa cgtgacactg taacctatta ttgccggatt gcgcgtaatc
    43321 gtcaccatcc ggcaatatta cggtgatcct atcgaaacag cggtttaacc gcgacaggaa
    43381 ttaacagttg tgattgccat tgcgccagcg ttaagcgagc cagttcaccc agtgcacggt
    43441 agaaagggtg ttctgctttt tcgataaaaa catcaagaaa acgtgttgac cacggaaaaa
    43501 ggtgccatgc cagcaattct tcgcattctg tctggcgacc attttccgcc aaccacgcag
    43561 ccatcaacag cagtgaacca aaatgatctt ccggttcgtt ttgcttcatt tcaaactgaa
    43621 tgcctttctc gcgcatccac tgacgaagtg ccaatgttga atcgccaaac agcacagatt
    43681 cgcgatccag ccagaccgac ccccacggcg gagacggcag tgcccacggg ccgacaaaca
    43741 aacgctgcca ggcctgggcg tgagtctctt cacactgtgt ctgaaaagcc gtcaccagcg
    43801 gcgctaatga cgcttctggt agaggccact gagtttccca gccgtcactg gtgagtaccg
    43861 caaccagagg tgcggcttcc gcactctctg gagcgtaata aaacagcgcg cccaacacgc
    43921 gcgccgcgac agaaaaatta tcttgctgtg aaaaatgggt cattcctaac atcctgaaat
    43981 gtgcgggttg ccccgcacca atttgttaac ctgcaatagc cattcctact gtcatgtgca
    44041 ggccataaaa gagcacacgc ccgatcatct caccgccaag tatgaggatt agccccagaa
    44101 taagtccggc aacgtgaggt tcacggcgac gaatgagcgg gcaaagccag catccaagac
    44161 ctgcgcataa caataccaca cgccagacct gtaaggaggc gtaatctggt accagtgcgc
    44221 tggcttgttg cacggatgag tgaatagacg ccagagacaa accttgcagc acaatcaccc
    44281 cggcacaagc gatcagcgcc agaacgctaa tgatggcaaa tggtgtggta ttaaatgtaa
    44341 cgcgggctgc ccgcagaatt gcggcagcaa gtattggacc gctcaacaat accgtcagga
    44401 agaaagccag cgtagtgtaa ccgttatgcc aggttggcac agtgtcgatt tgatacacgc
    44461 aggtcatcat ccagacgaaa atgacgccga gcgccatgct gacaagtaac cagagtttcc
    44521 ccaacgcttg cggcatttta ccgatgaccg ccaccagcca ccatagtccg ccaacggcaa
    44581 aaaatatgga accggcagca atctcgttac tcagaccaga agctccgatt cgattcagcg
    44641 agttaaacgc ccgcagtggc gatcccaggt gcatgacaga ggcaataaac ccgatgccca
    44701 tcaacagcca gaggaaaaac atgccacgga caatacgctg acggtctgcg tcatttttgg
    44761 cggcgaacca gccaattccg ctaacaatca gcgcgcctac tacgcattga cccaatacgg
    44821 taaagataac cagtggccac tcatgccatc catttcccat tttacacttc ctccggatta
    44881 gccagataac cagtggtatc gccggtcggg cggctgttgg cgttaggttt gataacgata
    44941 ttgggttttg tgaaatgcgc gcgcggcagt ggcgcgacgg cagccagagt gccgtgcttc
    45001 tggcgcagct cttcaattgg accgaactca agtgcacgca gagggcagga ttcgacacat
    45061 atgggttgtt tcccctcggc gacgcgcgaa taacaaccat cgcacttcgt catgtgccct
    45121 ttttcagcat tgtactgtgg cgcgccgtac ggacaagcca tgtggcagta gcggcagccg
    45181 atacaaacat cttcatcaac caccacaaag ccatcttcac gcttatgcat tgcgccgctg
    45241 gggcagactt ttgtacatgc ggggtcatcg caatggttac aggagatgga gagataataa
    45301 gcgaacacgt tctggtgcca gacgccatta tcttcctgcc agtcgccgcc agcgtattca
    45361 taaatacggc ggaaactgac ttccgggcca agatctttaa aatctttgca cgccagttcg
    45421 caggttttac agccagtaca gcggctggaa tcgataaaaa atccatattg tgtggtcatg
    45481 ggctactcct taaacctttt cgatctggac aagattgctg tgcgacgggt ttccctttgc
    45541 cagcggtgaa gggcggtgag aggtcagaat attgatactg ccgccgtgat cgacccggtc
    45601 accaaacata tccgctttaa gccacgcacc ttgcccgatg gcggtaacgc caggcagaat
    45661 acgcggagtc acttttgcgg caatcagcat ttctccatta ttgttaaata cccgcacggt
    45721 atcgccatga cggataccgc gtgcctgagc atcaatgggg ttgatccaca cctcttgtgg
    45781 gcaggcctgc tgtaacacat caatattgcc gtagctggag tgggtacgcg ctttgtagtg
    45841 gaagcccgtt aactgcagtg gataggtttt ccgcagggga tcgtcccagc catcaaaacc
    45901 tggggtatac gcaggaaggg gatgaataat ttcatctttt ttcaattccc aggtatctgc
    45961 aatcttcgcc agtcgttccg aataaatttc gattttcccc gaaggtgttt tcaacgggtt
    46021 tgcctgtgga tcttcacgga atgcgcggaa agcgacgtag tgttcttccg ggcatttttt
    46081 cttaaagatc ccggtcgttt tcatctcctc gtagtcgggc atctcagggt tacgttcctt
    46141 cgttttcgca tggagatatt tgatccattc atgctgactg cgaccttcag taaaggtttg
    46201 ataaacgtct ggtcctaagc gtttggcgac ttcactcagc atccagtaga tgggtttgcg
    46261 ttcaaatttt gctgaggttg cgggttgggc gaggatcaca tagcccatat tccctgcaga
    46321 ttcatgagag ataaggtctt cttgctctgt tggcatcagg tcgggcaaca ggatatcgca
    46381 atacttagcc gaggccgtca tgaagtggtc aatgccaaca atcatctcgc acttgctgtc
    46441 atcctgaagc acctcatggg tgtgattgat gtcgccatgt tgattgatca atgtgttact
    46501 ggcgtagcac cataaaaact tgatggggac atccagtttt tcttttccac gaacaccatc
    46561 acgggtcgcg gtcatttccg taccatggtc gatggcatct gtccatgtaa agacggaaat
    46621 ctgcgtttta acaggattct caagcatcgg gaaccattct acccccagat cccagctacc
    46681 ttcgcgtaca cctgagttgc cgccgtttat gccgacgtta ccggtgagaa cggaaagcat
    46741 ggcaatagcg cgggacgttt gctcgccgtt ggaatgtcgt tgtggccccc aaccctgaca
    46801 aatataagca ggttttgctg aaccgatctc tcgtgccaac tggataattt tttctgccgg
    46861 gatgctggtg atttttgctg cccattccgg cgttttagct atgccgtcag gcccttcgcc
    46921 cagaatatag gctttataat gcgcgttacg tggtgcgttg gcgggcagcg ttttttcatc
    46981 gtaaccaaca caatatttgt cgagaaatgg ctgatcgacc atgttttcag taatcagtac
    47041 ccaggcaatc gcacaggcca gtgcgccatc ggtgccaggg cgaatgggca gccattcatc
    47101 ttcacgcccg gcagcagtgt cgttataacg tggatcgatg acgatcatgc gtgcgtttga
    47161 acgttcgcgg gcttgctcga cgtagtaagt gacaccaccg ccgctcatcc gcgtttctgc
    47221 cgggttattt ccgaacataa cgaccagttt cgtattggcg atatcatccg ggctgttgcc
    47281 atcattggca ccgaacatat aactcattgc ggcactgatc tgtgcggtac tgtagctgcc
    47341 atagcgactg agaaaaccac cgcaagagtt catcagacgg tacgggacgt ttgagttggt
    47401 gatgtttccg ccatctacgc ctgttccgta caggacatgt acagcctcat tgccgtaatc
    47461 tttcaggatc cgccgaagat tatcactgat ggtatccagg gcttcgtccc aacttatccg
    47521 ttcaaattta ccttcaccgc gcttgccgac gcgcttcatg ggatatttca acctatcagg
    47581 atgattcatc cgtcggcgga tagagcgccc gcgtaaacac gctcgaacct gatggttacc
    47641 gtagacgtcg tcacctgtcg tatcagactc cacccagtac acggtgtcat ctttcacatg
    47701 caaacgtaac agacagcggc tcccgcagtt aacggtgcag gaactccaga ccgctttctc
    47761 ttctaccgga gcctctgccg cccggaccat ttgggaaaat ggcagagtga aagcactgct
    47821 tgccagcgca agactgccaa gtgcggaggt tttcatcaga cttctacggc tgatttcagc
    47881 cttcatgagc gcctctgtgg tatggatttt catcattact cacttattgc ttttcaaaca
    47941 aaatgtcatg ccagaattta tggttgtcgt gggttatatt ttttgtaagc gtcaacggag
    48001 caccgtattg acgcttattt attggtgagt actacgttcc atggcaggag ttcgtcaact
    48061 cggttggaag gccattccgg cagtacgctc aggatatggc gcagatacgc ttccggatcg
    48121 ataccgttca gacggcaggt gccgatcagc ccgtacaaca gtgctccacg ctcgccgccg
    48181 tgatcgctgc caaagaacat aaagtttttc tttccgagac agactgcacg aagcgctctt
    48241 tccgcagcat tattatccgc ctccgccaga ccgtcatcac tgtaataaca gagggcatcc
    48301 cactgattca gtacatagct gaacgcttcg cccagtctgg attttttcga cagcgtacca
    48361 ttcttctcca ccatccattc atgcagcgac gtcagtaaca ctttgcttcg ctgctgcctg
    48421 acggcaagac gctctgactc cggtaatccc cgtatttcat cctcgatggc gtacagttca
    48481 ctgattcgct tcagggcttc ttctgccgtc gcacttttgc tgctgatgta tacatcgtgg
    48541 atttttcgcc gggcatgggc ccagcacgca acttctgtca gtgcaccacc ttcacgttct
    48601 gcactgaaca acctgtcgta acctgtgaac gcatccgcct gcaggatacc ccggaagggg
    48661 cggaggtgtt gctccgggtg tttcccctgc cggttcggcg agtacgcgaa ccagaccgct
    48721 ggaggagatg acgaacccac attgcgatca tcccggacat acgtccagat acgccctgtt
    48781 ttcgcctttt tctgacccgg tgacagtacc tttaccggtg tgtcatcagt gtgaaccttg
    48841 cgggtgttca ttacataacg gtacagggca tcattcaccg gtgtcattaa ctggcagcac
    48901 gcgtcaaccc agttggagag taaggcccgg ctcagttcga caccctggcg ggcaaagatt
    48961 tcactctgac gatacagtgg cagatgttcg cagtattttc ccgttaacac gcgggcaagt
    49021 aatccggggc ccgcgatacc acgctctatc gggcgggacg gcgccggtgc ttcaacaata
    49081 cagtcacatt ttgtacaggc tttttttacc cgttctgtgc ggatcacttt cagggcactg
    49141 ctcaccagtt ccagctgttc agcgctgact tcccccagat aatccagctc accgccacac
    49201 tccgggcaac agctttcttc tggctccagg cggtgtattt cacggggaag gtgtgccggt
    49261 aacggacgac gatggcgcga ctgtcgcaac tggcggggaa cctgaggatc gtcttcccgc
    49321 ccactgtaac gatcgctgtc ctgttcacgt tgtttcagca gagcctcagc cagttcaact
    49381 tcacgacgca gtttttcaga acgggtaccg aacagcatcc ggcgcagttt ttctatctga
    49441 gcccgcagat gttctatttc ccgttcatct tcttcgatct tttcttcggc acgtgtcagt
    49501 gcagagcgca ggaaggcttc cgtctcttca accagactca gttgctggtc tttctgacgg
    49561 agggcttcag cctgctcaga gagcaacctt tccagctctg cgatgcgaat gaggtatttc
    49621 tgactcatga ccgtttttat aatgcggtca ggagtttttt acaacattgt cagtgagtta
    49681 cggctggatg tttttggctg acgccagtcc agcttatcga ggagcattgc cagttgcgag
    49741 cgggtaatgg ataccttgcc gtcacgtacc gcaggccaga taaactggcc ttcctccagg
    49801 cgtttggtga acaggcacag accatcagca tcagcccaaa gaattttgac ggtgtcaccc
    49861 cgtcggccac ggaagataaa caggtgaccg gagaagggat tatcattcag cacatgttgt
    49921 acctgttctc ccagtccgtt gaaggattta cgcatatcgg taacgccggc aacgagccag
    49981 atacgggtac ctgatgggag tgagatcatc ttcccctccc ggtcagttca cggatcaaca
    50041 ctgtgagcag ctctggcgat ggattttcca gcgtcatgtt accgtgacgg aattccacct
    50101 tgcaggaact ggcactgact ctggtctgag tggaagtgga taaagacggc gcaatggccg
    50161 ccacaggttc tttctgctca tccggcgtta tttctacagg taataattca acgccagtgt
    50221 cagaagaggt cgttaccgga agacgccgcg aaacacgccc ttcgttctgc cagagcctga
    50281 gccatttgaa aataacatta tcattgacgc cattttcacg tgcaatctgt gcaacacaag
    50341 ctccaggttg tgatgccagt tccaccatac gaagtttgaa ttcattcgaa tagtttttac
    50401 gaggttcttt tcgccagtcc tgtaattcca tacttagatg tccgtctata tcagatgggc
    50461 gtctaagtta ccaattctcg tctgatggct acatacggcg gtcagtttac gcttaccgta
    50521 caactgactg atctgctcca gggcttcttc cgtcagtact gacgggatgc ggacgtgcac
    50581 atcgtggatc tttcggcggg catgagccca gcaggcagct tccgttatcc caccattgcg
    50641 atacagctcg ttgaacccgg cgtacgcatc cgcttgcagc acaccgctga agcaggcaag
    50701 atgagtctgc ggatggatgc cttttctgtc cgggctgtaa gcgaaccaca ctgcaggtgc
    50761 caacgctgac ccggcattgc ggtcatcacg aacatacgcc cacaaccgcc cggtcttcgt
    50821 cttcttatta cccggcagca gtacctggac cggggtatca tcggcatgga gtttgccgtc
    50881 agtcatgaca tagccatgaa gcgcctcttc cag
//
LOCUS       DAPHDW010000016        50858 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.016, whole genome shotgun sequence.
ACCESSION   DAPHDW010000016 DAPHDW010000000
VERSION     DAPHDW010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 50858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 50858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50858
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.016"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..326)
                     /locus_tag="RH057_000896"
     CDS             complement(<1..326)
                     /locus_tag="RH057_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain containing protein"
                     /protein_id="HDV3127308.1"
                     /translation="MNLEKGGRGAIERMVEAYGFKTRQALCDHLGISKSTLATRYMRD
                     SFPAEWVIQCALETGTSLNWLTTGHGSKQTSGNTNTMEVAKYVLSDGALCEDGFYIFD
                     REFLPSA"
     gene            452..673
                     /locus_tag="RH057_000897"
     CDS             452..673
                     /locus_tag="RH057_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001247707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HDV3127309.1"
                     /translation="MRPNISITLTTPHVTIERYSELTGLSIDTINDMLADGRLIRHRL
                     RKDKKREKVMINIAAMTVDALSECNLNLN"
     gene            706..1215
                     /locus_tag="RH057_000898"
     CDS             706..1215
                     /locus_tag="RH057_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory CII family protein"
                     /protein_id="HDV3127310.1"
                     /translation="MFDYQVSKHPHFDEACRAFALRHNLVQLAERAGMNVQILRNKLN
                     PAQPHLLTAPEIWLLTDLTEDSTLVDGFLAQIHCLPCVPINEVAKEKLPHYVMSATAE
                     IGRVAAGAVSGDVKTSAGRRDAISSINSVTRLMALAAVSLQARLQANPAMASAVDTVT
                     GLGASFGLL"
     gene            1223..1423
                     /locus_tag="RH057_000899"
     CDS             1223..1423
                     /locus_tag="RH057_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2724 domain-containing protein"
                     /protein_id="HDV3127311.1"
                     /translation="MLTKEPSFASLLVKQSPAMHYGHGWIMGEDGKRWHPCRSQDELL
                     AELSTKKRGNKWLLKALRRLFH"
     gene            1387..1728
                     /locus_tag="RH057_000900"
     CDS             1387..1728
                     /locus_tag="RH057_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5347 domain-containing protein"
                     /protein_id="HDV3127312.1"
                     /translation="MAIEGAAATVPLSPGERLNGLNHIAELRAKVFGLNIESELERFI
                     KDMRDPRDINNEQNKRALAAIFFMAKIPAERHSISINELTTDEKRELIKAMNHFRAVV
                     SLFPRRLTMPN"
     gene            1796..2029
                     /locus_tag="RH057_000901"
     CDS             1796..2029
                     /locus_tag="RH057_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2732 family protein"
                     /protein_id="HDV3127313.1"
                     /translation="MRNIEILTTKTGPDDAGLNILLTEARLEERRARAEAMAARLDSL
                     ACHISSRQLNHVEAAELLRVTAEAIQNEAQEIH"
     gene            2029..2256
                     /locus_tag="RH057_000902"
     CDS             2029..2256
                     /locus_tag="RH057_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HDV3127314.1"
                     /translation="MADAMDLVQQRVEEERQRHIRAARAKTPGVSRVLCIECEAPIPP
                     ARRRAIPGVQLCITCQEIAELKGKHYNGGAV"
     gene            2253..3110
                     /locus_tag="RH057_000903"
     CDS             2253..3110
                     /locus_tag="RH057_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000104187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA adenine methylase"
                     /protein_id="HDV3127315.1"
                     /translation="MSTILKWAGNKTAIMSELKKHLPAGPRLVEPFAGSCAVMMETDY
                     PCYLVADINPDLINLYKKVAADCEVFISRARALFEDANREVTYYNIRQEFNYSTEITD
                     FMKAVYFLYLNRHGYRGLCRYNKSGHFNIPYGNYKNPYFPEKEIRAFAEKAQRATFIC
                     ASFDETLAMLQVGDVVYCDPPYDGTFSGYHTDGFTEDDQYHLASVLEHRSSEGHPVIV
                     SNSDTSLIRSLYRNFTHHYIKAKRSIGVAAGESKSATEIIAVSGPRCWVGFDPSRSVD
                     SSAVYGVRA"
     gene            3107..5521
                     /locus_tag="RH057_000904"
     CDS             3107..5521
                     /locus_tag="RH057_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDV3127316.1"
                     /translation="MSHADMNNCSGFNEVAAAFSWNSPKKAINPYLDPAEVAPVSALS
                     NLITLYATDNEQEQLRREALSDQVWERYFFNESRDPVQREMEQDKLISRAKMAHEQQR
                     FNPDMVILTDVNAQPSHISKPLMQRIEYFSSLGRPKAYSRYLRETIKQCLERLEHVRE
                     SQLSTSFRFMASHEGLDGLLILPEMSQDQVKRLSTLVAAHMSMCLDAACGDLYATDDV
                     KPEEIRKTWERVAAETLRLDVIPPAFEQLRRKRNRRKPVPYELIPGSLARMLCADWWY
                     RKLWKMRCEWREEQLRAVCLVSKKASPYVSYEAVMHKREQRRKSLEFFRSHELVNEDG
                     DTLDMEDVVNASSSNPAHRRNEMMACVKGLELIAEMRGDCAVFYTITCPSRFHSTLNN
                     GRPNPTWTNATVRQSSDYLVGMFAAFRKAMHKAGLRWYGVRVAEPHHDGTVHWHLLCF
                     MRKKDRRAITALLRKFAIREDREELGNNTGPRFKSELINPRKGTPTSYIAKYISKNID
                     GRGLAGEISKETGKSLRDNAEYVNAWASLHRVQQFRFFGIPGRQAYRELRLLAGQAAR
                     QQGDKKAGAPVLDNPRLDAILAAADAGCFATYIMKQGGVLVPRKYHLIRTAYEINEEP
                     TAYGDHGIRIYGIWSPIAEGKICTHAVKWKMVRKAVDVQEAAADQGACAPWTRGNNCP
                     LAENLNQHEKDESADGDTRTDITCMDDKELHDYLHSMSKKERRELAARLRLVKPKRRK
                     DYKQRITEYQRQQLVYELKSRGFDGSEKEVDLLLCGGSIPSGAGLRIFYRNQRLQEDD
                     KWRNLY"
     gene            5675..5863
                     /locus_tag="RH057_000905"
     CDS             5675..5863
                     /locus_tag="RH057_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127317.1"
                     /translation="MQDYFLESLKLQRIDFFLKLVAASECSDEEKGLALQWVSELTDE
                     LMAKIRSHEYSRSMDVIS"
     gene            complement(5931..6230)
                     /locus_tag="RH057_000906"
     CDS             complement(5931..6230)
                     /locus_tag="RH057_000906"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127318.1"
                     /translation="MKRITFSFTLVAIIIALAGCALPDKDGDFGAYVHACQQYAYGKS
                     YAFENRDFAYKVCKDAAKLWSEEVPGYIIRQIQLHPEIPSEEIKYAAMAGSLGNN"
     gene            complement(6339..7217)
                     /locus_tag="RH057_000907"
     CDS             complement(6339..7217)
                     /locus_tag="RH057_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127319.1"
                     /translation="MAMSLSCPLILLGLALFFYDWDRDMKKLTGVIAFALLLTACDKP
                     KIDASSDQSMKESIQKVRESLPADKKAQFDDAVKVVAFSQINMRELMQAGTSSGDVYE
                     TKIKSALEGKTGDEVINYAQTIRLEREKREKEQALQEIKELEAKQTSATQAAEKMKAF
                     KVERSRFYFQKEDYGNDQPILDISVENGTDKAVARVFFKGVIASPGRSVPWFSDVFNY
                     KISGGLEPGEKANWKLAPNRYSDWGKLKVPADAIFTVTVIGLEDADGKSIYGDAEFSE
                     RDADRLNQLRDKYLSK"
     gene            7805..8779
                     /locus_tag="RH057_000908"
     CDS             7805..8779
                     /locus_tag="RH057_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004205800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127320.1"
                     /translation="MGLGLKDAIVHTAAFHQKETNELQLPNNHWHPGFITVLAAYVNH
                     HKITEDNCVLSCPGYMRAINLHGALWGEDQYQQERVNVGRNYSLVTALTNVEAVDVAT
                     GSINSCVRQLTFPDRDPRDCPKGLTDLTHVIGELHDNVWSHGKSTGFSFAQRSAVPHT
                     QKKEHYLEFSLADCGLGFLRELRRAGIPGIETHQDAIAWCIQEGHSSKHADLQDDWAQ
                     QLPQDFIGGSVFGHGVSVKEKDNNHQGLGLYHLMKLIKTYNGELQLASGNVCLEAIGD
                     EVSYTELRTEWPGVAISCRFKIHELAVDKDSEENDPQLMEIMRALGGE"
     gene            8779..9117
                     /locus_tag="RH057_000909"
     CDS             8779..9117
                     /locus_tag="RH057_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="STAS-like domain-containing protein"
                     /protein_id="HDV3127321.1"
                     /translation="MNKIAYKLPEGDLASRNQAIPQRHKIEGFIKEGYTVDLDLSGVY
                     SISESYSDEIFGVLVVKFGVTKVLSQVKVRNAPPSILKSIAKVIQRRSNEVASKKVHS
                     VGFDGLYAVC"
     gene            complement(9164..10201)
                     /locus_tag="RH057_000910"
     CDS             complement(9164..10201)
                     /locus_tag="RH057_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV3127322.1"
                     /translation="MGKSKKNRAAATKQSQLKSQTTAEAFSFGDPIPVLDRRELLDYV
                     ECVQTDRWYEPPVSFDGLARTFRAAVHHSSPIAVKCNILTSTYIPHPLLSQQAFSRFV
                     QDYLVFGNAYLEKRTNRFGEVIALEPALAKYTRRGLDLDTYWFVQYGMTTQPYQFTKG
                     SIFHLMEPDINQEIYGLPGYLSAIPSALLNESATLFRRKYYINGSHAGFIMYMTDAAQ
                     NQEDVNNLRNAMKSAKGPGNFRNLFMYSPNGKKDGLQIIPLSEVAAKDEFLNIKNVSR
                     DDMMAAHRVPPQMMGIMPNNVGGFGDVEKASLVFVRNELMPLQKRLHELNEWVGAEVI
                     RFDDYVLGVQE"
     gene            complement(10201..11967)
                     /locus_tag="RH057_000911"
     CDS             complement(10201..11967)
                     /locus_tag="RH057_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDV3127323.1"
                     /translation="MNTTLTPADLDPRRQAMLLYFQGYRVARIAEMLGEKVATVHSWK
                     KRDKWGDYGPLDQMQLTTAARYCQLIMKEHKEGKDFKEIDLLARQSERHARIGKFNNG
                     GNEADLNPNVANRNKSPRRQPEKNVFTDEQIEKLEEIFHSSMFNYQRHWWEAGKTNRI
                     RNLLKSRQIGATFYFAREALIDALLTGRNQIFLSASKAQAHVFKQYIIDFAKEVEVEL
                     KGDPMVLPNGATLYFLGTNARTAQSYHGNLYLDEYFWIPKFQELRKVASGMAIHKKWR
                     QTYFSTPSSLTHSAYPFWSGALFNRGRNKADKVDIDLSHSNLAPGLLCADGQYRQIVT
                     VEDAVRGGCNLFDLDQLRMEYSPDEYQNLLMCEFVDDLASVFPLSELQACMVDSWEVW
                     TDFHALALRPFGWREVWIGYDPAKGTQNGDSAGCVVVAPPAVPGGKFRILERHQWRGM
                     DFRAQADAIKKLTEQYNVTYIGIDSTGVGHGVYENVKAFFPAVREFVYNPNVKNALVL
                     KAYDIISHRRLEFDAGHTDIAQSFMAIRRATTASGNRPTYEASRSEEASHADLAWATM
                     HALFNEPLQGESANTSNIVEIF"
     gene            12110..12943
                     /locus_tag="RH057_000912"
     CDS             12110..12943
                     /locus_tag="RH057_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPO family capsid scaffolding protein"
                     /protein_id="HDV3127324.1"
                     /translation="MTVKAKRFRIGVEGATTDGREIQREWLEQMAASYNPAVYTALIN
                     LEHIKSYLPDSTFNRYGKVTALFAEEITEGPLAGKMALYADVEPTESLVELVKKGQKL
                     FTSMEVSPKFADTGKAYLVGLAATDDPASLGTEMLTFSASAAHNPLANRKQNPANLFT
                     AAEETVIELEEIQDDKPSLFARVTALFTKKEQSDDARFSDVHKAVELVATEQQNLSAR
                     TEKSLSEQEERLSELETALQAQQTAFNELVDKLSHEDSRQDYRQRATGGNAPADTLTN
                     C"
     gene            12960..14018
                     /locus_tag="RH057_000913"
     CDS             12960..14018
                     /locus_tag="RH057_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein, P2 family"
                     /protein_id="HDV3127325.1"
                     /translation="MKKNTRFAFNAYLQQLARLNGVAVEELSSKFTVEPSVQQTLEDQ
                     IQQSAAFLTLINVTPVTEQSGQLLGMGVGSTIAGTTDTTAKEREPVDPTLMVDVEYKC
                     EQTNFDTVLTYAKLDLWAKFQDFQVRIRNAIVKRQALDRIMIGFNGVKRAKTSNRSEN
                     PLLQDVNKGWLQKIREDAPDHVMGSTTTGGETTPGAVKVGKGGEYANLDAVVMDAVNE
                     LIDVVYQDDDDLVVICGRELLSDKYFPLVNKEQENSEKLAADMIISQKRMGGLQAVRA
                     PFFPPNALLITRLDNLSIYWQEDTRRRSVIDNPKRDRIENFESVNEAYVVEDYRCAAL
                     VENIQIGDFSAAAAETGA"
     gene            14022..14672
                     /locus_tag="RH057_000914"
     CDS             14022..14672
                     /locus_tag="RH057_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase endonuclease subunit"
                     /protein_id="HDV3127326.1"
                     /translation="MSLSPARQHRLRVQAEQAAREGGSVRHASGYDLMLLQLAEDRRR
                     LKGVQSTVKKAEIKVELLPKYAAWAEGVLAAGGAQQDDVLMYVMLWRIDAGDYAGALE
                     IGRHALRHGWVMPLGNRNVQTVLAEEMADAAQSAMLATTGFDADLLLQTLELTDGLDM
                     PDQSRARLHKAIGAVLSESNPASALNHLNHALQLDPRCGVKKDKQQLERRLRNDSR"
     gene            14768..15232
                     /locus_tag="RH057_000915"
     CDS             14768..15232
                     /locus_tag="RH057_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head completion/stabilization protein"
                     /protein_id="HDV3127327.1"
                     /translation="MKFVAPEQAPEQAEIIRNTPFWPDVDLSEFRSVMRTDGTVTQPR
                     LKQVALSAISEVNAELYEFRRRQQMLGYASLAEVPAEQLDGKSERIQHYFNAVYCWAR
                     AMLNERYQDYDATASGVKRGEELAEASGDLWRDARWAISRVQDAPHCTVELI"
     gene            15232..15435
                     /locus_tag="RH057_000916"
     CDS             15232..15435
                     /locus_tag="RH057_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail protein X"
                     /protein_id="HDV3127328.1"
                     /translation="MKVRAHQYDTVDALCWRHYGRTQGVTEQVLKANPGLAEYGPFLP
                     HGLQVELPDIPTTTTVQTVQLWD"
     gene            15439..15654
                     /locus_tag="RH057_000917"
     CDS             15439..15654
                     /locus_tag="RH057_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127329.1"
                     /translation="MTLERISAFITYCIAVVLAWLGDLSIKDASTLGGLMIGVLMLAI
                     NWYYKHKAYQLLRDGQISREDYESINR"
     gene            15635..16147
                     /locus_tag="RH057_000918"
     CDS             15635..16147
                     /locus_tag="RH057_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV3127330.1"
                     /translation="MNPSIVKRCLVGTVLAIAATLPGFQQLHTSVEGLKLIADYEGCR
                     LQPYQCSAGVWTDGIGNTSGVIPGKTITERQAAEGLISNVLRVERALERCVKQQPPQK
                     VYDSVVSFAFNVGTGNACSSTLVKLLNQRRWADACRQLPRWVYVKGVFNQGLDNRRAR
                     EMAWCLQGAN"
     gene            16149..16526
                     /locus_tag="RH057_000919"
     CDS             16149..16526
                     /locus_tag="RH057_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000730949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127331.1"
                     /translation="MKKKLISGLFLMLWMALLIAAMVYPQGIFPVLAASGVWVACLLT
                     WAVIPVALAALIQNGPLWQELRASLLKTITRKENVFISWVMRLLIVVSLAWTGWAITL
                     VFYLLTVIAFWITRNQMAQQVAA"
     gene            16523..16951
                     /locus_tag="RH057_000920"
     CDS             16523..16951
                     /locus_tag="RH057_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001341071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysB family phage lysis regulatory protein"
                     /protein_id="HDV3127332.1"
                     /translation="MNRLLLVVLALLLAALGWQTWRLADASQTISTQADELQSKSQAL
                     AKSNSQLISLSILTETNNREQARLYAEAEQTSALLRQRQRRIEKLKRENEDLRRWADT
                     PLPADIIRLRERPTLTGGAAYRQWLSASDAVSAGAGSTAH"
     gene            17047..17478
                     /locus_tag="RH057_000921"
     CDS             17047..17478
                     /locus_tag="RH057_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3127333.1"
                     /translation="MNKPQSLRHALNKAVPYVRNNPDKLHLFVDNGSLVATGASSMSW
                     EYRYTLNVVIEDFSGDQNLLMAPVLLWLRDNQPDAINNPALREKLFTFEVDILRNDVC
                     DISLNLQLTERVLVSTDGSVSSVEAIAEPDEPEEMWTVKRG"
     gene            17471..17917
                     /locus_tag="RH057_000922"
     CDS             17471..17917
                     /locus_tag="RH057_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage virion morphogenesis protein"
                     /protein_id="HDV3127334.1"
                     /translation="MAELQKVDDWLSALLANLEPAARSHMMRQLAQELRRTQQQNIRM
                     QRNPDGSSYEPRRVTARSKKGRIKRQMFAKLRTTKYLKTSASADSASVQFEGKVQRIA
                     RVHHYGLRDRVSRKGPEVRYAERRLLGVNDDVEAMTRDMILQWLAG"
     gene            17986..18564
                     /locus_tag="RH057_000923"
     CDS             17986..18564
                     /locus_tag="RH057_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001552634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate assembly protein V"
                     /protein_id="HDV3127335.1"
                     /translation="MNAQLTEIMRLITNLIRTGVVTEVDRENWLCRVKTGELETNWIS
                     WLTLRAGNARTWWRPSEGEQVVLLSLGGNLETAFALPAVYSNQFAPPSTSADACVTEH
                     PDGGWFEYEPATGRWYVRGIKSMVIEAADNITMKTSEFVLEADRTRINSEVVINGGVT
                     QGGGAMSSNGIVVDAHQHTGVLKGGDTTGGPV"
     gene            18561..18920
                     /locus_tag="RH057_000924"
     CDS             18561..18920
                     /locus_tag="RH057_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HDV3127336.1"
                     /translation="MTLYSGMNNTSGKVITDIDHLRQSVRDILLTPQGSRIARREYGS
                     LLSALIDQPQNPALRLQVMSAVYVALSRWEPRLTLDSITINSNFDGSMVVELTGRRNN
                     GVPVSLSVSTGAENGSD"
     gene            18907..19815
                     /locus_tag="RH057_000925"
     CDS             18907..19815
                     /locus_tag="RH057_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate assembly protein"
                     /protein_id="HDV3127337.1"
                     /translation="MAVIDLSQLPAPQIVDVPDFETLLAERKAEFVALHPKDEQEAVI
                     RTLELESEPVTKLLQENAYRELLLRQRINEAAQAVMVAYAMGGDLDQLAANYNVTRLT
                     VTPADNDAVPPVAAVMESDEALRLRVPAAFEGLSVAGPTAAYEFHARSADGRVADASA
                     TSPAPAEVVLTVLSREGDGTAEKDLLDVVEKALNSENVRPVADRLTVRSAEIIPYRVE
                     ATIFLYPGPEAEPVMAAAKASLQKYIASQTRLGRDIRRSAIFAALHVEGVQRVELASP
                     LADVVLNKTQAASCTQWSVTNGGTDE"
     gene            19808..20413
                     /locus_tag="RH057_000926"
     CDS             19808..20413
                     /locus_tag="RH057_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein I"
                     /protein_id="HDV3127338.1"
                     /translation="MNSLLPPGSTPLERRLAQTCSGISDLQVPLRDLWNPATCPVSFL
                     PYLAWAFSVDRWDEGWTESVKRQVVKDAFYIHQHKGTTSAVRRVVEPFGFLIRIIEWW
                     QTGETPGTFRLDIGVQDQGITEDTYLELERLISDAKPCSRHMIGMSINLQTSGPHWVG
                     AASYLGEEITIYPYINETIISGGTAHEGGAVHVIDTMRVNP"
     gene            20410..21792
                     /locus_tag="RH057_000927"
     CDS             20410..21792
                     /locus_tag="RH057_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3127339.1"
                     /translation="MSTKFYTLLTDIGAAKLASAAALGVPLKITHMAVGDGGGVLPTP
                     DAKQTALVNEKRRAALNMLYIDPQNSSQIIAEQVIPENEGGWWIREVGLFDESGALIA
                     VGNCPESYKPQLAEGSGRTQTVRMVLITSSTDNITLKIDPAVVLATRKYVDDKALELK
                     VYADDQMAKHLAAPDPHSQYAPKASPTFTGTPKAPTPAAGNNTTQVATTAFVQAALTA
                     LINGAPATLDTLKEIAAAINNDPNFSTTINNALALKAPLSSPALTGTPTAPTAAQSVN
                     NTQIATTAFVKSAIAGMVGSAPAALDTLNELAAALGNDPNFATTMLNALAGKQPLDNT
                     LTNLSGKDVAGLLTYLGLGEAAKRNVGTGANQIPDMSSYASGSGWRKMPDGSIEQWGG
                     IRFPGEHEPVSANVSFPIPFTQTPNIIIVFDGGFGGGNMWGATNWSTTGFIAHCNYGL
                     EGGVFFAKGW"
     gene            21792..22235
                     /locus_tag="RH057_000928"
     CDS             21792..22235
                     /locus_tag="RH057_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3127340.1"
                     /translation="MMKYLYVNNLAYPYELQSLYVEKGEWPEEKGVDIDEVIFREYFY
                     DTPPEGKYRCVGEDGLPAWADIPPPTREEQIASAETKKQQLINQANEYINSKQWPGKA
                     SIGRLKGEELVQYNLWLDYLDALELVDTSGAPDIEWPTPPAVQAR"
     gene            complement(22207..22809)
                     /locus_tag="RH057_000929"
     CDS             complement(22207..22809)
                     /locus_tag="RH057_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3127341.1"
                     /translation="MEQAILGKDGYALNDGEIIVHNYDGETREYISTSTEYLAVGVGI
                     PACSCLDAPGTHKAGYAICRSVDLNSWEYVPDHRGEVVYSTETGDTKEITVPGDYPEN
                     TTTIAPLTPYDKWDGEKWMTDTEAQHSAALDAAEAQRQSLINAAMASISLIQLKLQAG
                     RKLTQAETTRLNAVLDYIDAVTATDTSTAPDVIWPELPEA"
     gene            complement(22810..23346)
                     /locus_tag="RH057_000930"
     CDS             complement(22810..23346)
                     /locus_tag="RH057_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
                     /protein_id="HDV3127342.1"
                     /translation="MPVSCYLCIFLNVGLGEGSALPVGVPVPWPSATPPTGWLKCNGA
                     PFSAEEYPKLAKAYPKLKLPDLRGEFIRGWDDGRGIDSARLLLSSQAASILEHNHEMH
                     GWTGNPLMARDVGELGTSSVFAVQLSIGDGGILYSWKDGSGTTNDSKRMDRTNHVSSG
                     AGDGSPRNIAFNYIVRAA"
     gene            23377..23943
                     /locus_tag="RH057_000931"
     CDS             23377..23943
                     /locus_tag="RH057_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016529756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3127343.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNALNCAGCEQIFEDKISGTKSDRPG
                     LKKLLRTLSAGDTLVVWKLDRLGRSMRHLVTLIEELRQRGVNFRSLTDSIDTSTPMGR
                     FFFHVMGALAEMERELIVERTRAGLAAARAKGRVGGRRPKLTTEQWAQIGRLLEAGES
                     RQRIALIFDVGVSTIYRKFPANKINESP"
     gene            24086..25258
                     /locus_tag="RH057_000932"
     CDS             24086..25258
                     /locus_tag="RH057_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath protein"
                     /protein_id="HDV3127344.1"
                     /translation="MAQDYHHGVRVVEVNEGTRSITTVSTAIVGMVCTGDDADAKMFP
                     LNKPVLITDVLTASGKAGESGTLARSLDAIADQAKPVTIVVRVPQGETEDETTTNIIG
                     AVTAEGKKTGMKALLSAQSQLGVKPRILGVPGHDTKAVATELLSVAQSLRGFAYLSAY
                     GCKTVQEAITYRENFSQREGMLIWPDFTGWDTVLNAEATAYATARALGLRAKIDEQTG
                     WHKSLSNVGVNGVTGISADVFWDLQDPATDAGLLNQNDVTTLVRKDGFRFWGSRCLSD
                     DPLFAFENYTRTAQVLMDTMAEAHMWAVDKPLNPSLARDIIEGIRAKMRSLVSQGYLI
                     GGDCWLDESVNDKDTLKAGKLTIDYDYTPVPPLENLMLRQRITDQYLVNFASQVSA"
     gene            25268..25783
                     /locus_tag="RH057_000933"
     CDS             25268..25783
                     /locus_tag="RH057_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail tube protein"
                     /protein_id="HDV3127345.1"
                     /translation="MALPRKLKHLNLFNDGNNWQGIVESLTLPKFTRKYEKYRGGGMP
                     GAVDVDLGLDDSALDTEFSIGGTELLLFKQMGKATVDGIQLRFTGSIQRDDTGEVQAV
                     ELVVRGRHKEVDSGEWKTGESNTTKVTSTNSYAKLTINGEVLYEVDLINMVEIVDGVD
                     LMEAHRNALGL"
     gene            25838..26140
                     /locus_tag="RH057_000934"
     CDS             25838..26140
                     /locus_tag="RH057_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_461627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein"
                     /protein_id="HDV3127346.1"
                     /translation="MSDKQTEKTIQLDTPIKRGKTEITEIVLRKPQSGALRGTRLQAI
                     MDMDVNAMMTVIPRISSPALTAQEIAEMDPADLTAMSVEVVTFLLKKSVLAGLPTA"
     gene            26155..26274
                     /locus_tag="RH057_000935"
     CDS             26155..26274
                     /locus_tag="RH057_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GpE family phage tail protein"
                     /protein_id="HDV3127347.1"
                     /translation="MADIATIFHWPPSVTDVMPLTEVLEWRYKAIQRSGANDE"
     gene            26267..29344
                     /locus_tag="RH057_000936"
     CDS             26267..29344
                     /locus_tag="RH057_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HDV3127348.1"
                     /translation="MSDNNLRLQVILNAVDKLTRPFRSAQASSRELAAAVKKSRDAIK
                     QLDQAGSSLDSFRKLQAENQKLGDRLNYARQRANLLSQELGAMGPPSQRQVVALGRQR
                     LAVQRLEERQKKLQQQTALVRAELYRAGISAKDDAGATARLARETSRYNQELSKQEAR
                     LKRLGEAQRRMNAARASYARSLEVRDRIAGAGATTTAAGLAMGAPVMAAVKSYTSMED
                     AMKGVAKQVNGLRDDNGNRTARFYEMQDAIKAASEQLPMENGAVDFAALVEGGARMNV
                     ANPDDSWEDQKRDLLAFASTAAKAATAFELPADELSESLGKIAQLYKIPTRNIEQLGD
                     ALNYLDDNAMSKGADIIDVMQRLGGVADRLDYRKAAALGSTFLTLGAAPEVAASAANA
                     MVRELSIATMQSKSFFEGMNLLKLNPEVIEKQMTKDAMGTIQRVLEKVNALPQDKRLS
                     AMTMLFGKEFGDDAAKLANNLSELQRQLKLTAGNDALGSMQKESDINKDSLSAQWLLV
                     KTGAQNTFSSLGETLRQPLMDILYTVKSVTGALRRWVEANPELTGTLMKASAVVAAVT
                     VGLGTLAVALAAVLGPLAVIRLGFSVLGIKTLSSVTAAVTRTSSALSWLAGAPLALLR
                     RGLASSGNAAGLLTAPLSSLRRTASLTGNVLKTVAGAPVALLRSGLSGLRAVAVMFMN
                     PLAVLRGGLAAAGAVLRVLASGPLAMLRVALYAISGLLGALLSPIGLVVTALAGVALV
                     VWKYWQPITAFLGGVVEGFKAAAGPISAAFEPLKPVFQWIGDKVQALWGWFTDLLTPV
                     KSTSAELQSAAAMGRRFGEALAEGLNMVMHPLDSLKSGVSWLLEKLGIVSKEAAKAKL
                     PESVTRQQPATVNADGKVMMPSGGFPSWGYGFAGMYDSGGYIPRGQFGIVGENGPEIV
                     NGPANVTSRRNTAALAAVVAGMMGVAAAPAELPPLHPLALPTKGGEAIVSRAATVPLV
                     QRIEAPTQIIIQTQPGQSAQDIAREVARQLDERERRLKAKARSNYSDQGGYDA"
     gene            29341..29826
                     /locus_tag="RH057_000937"
     CDS             29341..29826
                     /locus_tag="RH057_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HDV3127349.1"
                     /translation="MMMVLGLYVFMLRTVPYQELQYQRSWRHAANSRVNRRPSTQFLG
                     PENDMLTLSGVLMPEITGGRLSLLALEQMAEQGKAWPLIEGSGTIYGMYVIEGLNQTK
                     TEFFRDGMPRRIEFTLSLKRVDESLSDMFGDLSAQLNNLQDTATSALSDISKMVGGLL
                     S"
     gene            29823..30923
                     /locus_tag="RH057_000938"
     CDS             29823..30923
                     /locus_tag="RH057_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage late control D family protein"
                     /protein_id="HDV3127350.1"
                     /translation="MNFSSELLNKGNKTPAFSISIEGRDITTVLDNRLMSLTLTDNRG
                     FEADQLDLELDDADGKIVLPRRGAVITLALGWKGQPLFPKGAFTVDEIEHTGAPDRLT
                     IRARSADFRETLNTRREKSWHKTTVGEVVKEIAARHKLKMALGKDLSDKPVEHIDQTN
                     ESDGSFLMRLARQYGAIASVKNGNLLFIRQGQGKSATGKPLPVITITRKDGDSHRFTL
                     ADRGAYTGVIASWLHTREPAKKESTTVKRKRRTKKQKKEPEAKQGDYLVGTDENVLVL
                     NRTYANRSNAERAAKMQWERLQRGVASFSLQLAEGRADLYTEMPVKVSGFKQPIDDAE
                     WTITTLTHTVSPDNGFTTSLELEVRIDDFEME"
     gene            31014..31232
                     /locus_tag="RH057_000939"
     CDS             31014..31232
                     /locus_tag="RH057_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001504085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDV3127351.1"
                     /translation="MMICPLCGSAAHTRSSFQVSSLTKERYNQCQNINCSHTFVTHET
                     FVRSIATPKESNPVQPHPMKSGQVALSL"
     gene            complement(31468..33153)
                     /locus_tag="RH057_000940"
     CDS             complement(31468..33153)
                     /locus_tag="RH057_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HDV3127352.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLRHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            33423..33800
                     /locus_tag="RH057_000941"
     CDS             33423..33800
                     /locus_tag="RH057_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HDV3127353.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(33830..34087)
                     /gene="grxA"
                     /locus_tag="RH057_000942"
     CDS             complement(33830..34087)
                     /gene="grxA"
                     /locus_tag="RH057_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HDV3127354.1"
                     /translation="MQTVIFGRPGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            34247..34534
                     /locus_tag="RH057_000943"
     CDS             34247..34534
                     /locus_tag="RH057_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HDV3127355.1"
                     /translation="MRAIGKLPKSVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNRPPQYSRSEREKDNDANH"
     gene            34518..35240
                     /gene="nfsA"
                     /locus_tag="RH057_000944"
     CDS             34518..35240
                     /gene="nfsA"
                     /locus_tag="RH057_000944"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HDV3127356.1"
                     /translation="MTPTIELICGHRSIRHFTDEPISEAQREAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALIAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKGALAQYDEQLAEYYLTRGSNNRRDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            35301..36203
                     /gene="rimK"
                     /locus_tag="RH057_000945"
     CDS             35301..36203
                     /gene="rimK"
                     /locus_tag="RH057_000945"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HDV3127357.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIHYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGDFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            36291..36767
                     /locus_tag="RH057_000946"
     CDS             36291..36767
                     /locus_tag="RH057_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HDV3127358.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            37118..38230
                     /gene="potF"
                     /locus_tag="RH057_000947"
     CDS             37118..38230
                     /gene="potF"
                     /locus_tag="RH057_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HDV3127359.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVSTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYANANKAATPLVSAEVRENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            38325..39458
                     /gene="potG"
                     /locus_tag="RH057_000948"
     CDS             38325..39458
                     /gene="potG"
                     /locus_tag="RH057_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HDV3127360.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEIVSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERHEDGLVLYSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            39468..40421
                     /gene="potH"
                     /locus_tag="RH057_000949"
     CDS             39468..40421
                     /gene="potH"
                     /locus_tag="RH057_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HDV3127361.1"
                     /translation="MSTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAVISTICCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            40418..41263
                     /gene="potI"
                     /locus_tag="RH057_000950"
     CDS             40418..41263
                     /gene="potI"
                     /locus_tag="RH057_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HDV3127362.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     AGWSTRWYGELLRDDAMMSAVGLSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARHG"
     gene            41323..41811
                     /locus_tag="RH057_000951"
     CDS             41323..41811
                     /locus_tag="RH057_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HDV3127363.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            41852..42979
                     /gene="rlmC"
                     /locus_tag="RH057_000952"
     CDS             41852..42979
                     /gene="rlmC"
                     /locus_tag="RH057_000952"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HDV3127364.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKPLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSETKLAQLRKALPWLQE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIASEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(43154..43885)
                     /gene="artJ"
                     /locus_tag="RH057_000953"
     CDS             complement(43154..43885)
                     /gene="artJ"
                     /locus_tag="RH057_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HDV3127365.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVIAKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(44177..44845)
                     /gene="artM"
                     /locus_tag="RH057_000954"
     CDS             complement(44177..44845)
                     /gene="artM"
                     /locus_tag="RH057_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HDV3127366.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(44845..45561)
                     /gene="artQ"
                     /locus_tag="RH057_000955"
     CDS             complement(44845..45561)
                     /gene="artQ"
                     /locus_tag="RH057_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HDV3127367.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(45568..46299)
                     /gene="artJ"
                     /locus_tag="RH057_000956"
     CDS             complement(45568..46299)
                     /gene="artJ"
                     /locus_tag="RH057_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3127368.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEASYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDGVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(46317..47045)
                     /gene="artP"
                     /locus_tag="RH057_000957"
     CDS             complement(46317..47045)
                     /gene="artP"
                     /locus_tag="RH057_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HDV3127369.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDLALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(47263..47778)
                     /locus_tag="RH057_000958"
     CDS             complement(47263..47778)
                     /locus_tag="RH057_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3127370.1"
                     /translation="MRYSKLTMLIPCALLLSACTTVTPAYKDNGTRSGPCVEGGPDNV
                     AQQFYDYRILHRSNDITALRPYLSDKLATLLSDASRDNNHRELLTNDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQSWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            47904..48227
                     /locus_tag="RH057_000959"
     CDS             47904..48227
                     /locus_tag="RH057_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HDV3127371.1"
                     /translation="MQFSTTPTLEGQTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGTDAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            48224..49054
                     /gene="amiD"
                     /locus_tag="RH057_000960"
     CDS             48224..49054
                     /gene="amiD"
                     /locus_tag="RH057_000960"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HDV3127372.1"
                     /translation="MRRVFWLIAAALLLAGCAGEKGIVEKEGYQLDTRRQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLVPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSAGVKYFAPFEPAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(49051..50064)
                     /locus_tag="RH057_000961"
     CDS             complement(49051..50064)
                     /locus_tag="RH057_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HDV3127373.1"
                     /translation="MKVLVTGATSGLGRNAIEFLCQKGISVRATGRNEAMGKLLEKMG
                     AEFVPADLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRDIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGVSKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(50163..>50858)
                     /locus_tag="RH057_000962"
     CDS             complement(50163..>50858)
                     /locus_tag="RH057_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HDV3127374.1"
                     /translation="FLNVITSVPPTTARALIQGLKHDLLADDTALRALIPQRLIAFDD
                     AVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKTSASLAALWQVVNQ
                     IGGKERYFFGNILWQTRALMDRAIGHKLAKGRPEREYLQTGDAVDSWKVIVVEPEKQL
                     TLLFGMKAPGLGRLCFSLEDKGDYRTIDVRAFWHPHGMPGLFYWLLMIPAHLFIFRGM
                     AKQIARLAEQSTD"
BASE COUNT        12302 a        12570 c        13919 g        12067 t
ORIGIN      
        1 gccgacggta gaaattctct atcgaaaata taaaaaccgt cttcacacaa ggccccatca
       61 gataatacat atttagcaac ttccatagta tttgtattac ctgaagtttg ctttgaacca
      121 tgcccggttg tgagccaatt aagcgaggtg cctgtttcaa gggcgcactg gattacccat
      181 tctgctggga atgagtcacg catgtagcgt gtggcgagtg tacttttaga gattcctaaa
      241 tgatcgcaca acgcctgtcg agtcttgaat ccataagctt ctaccatgcg ctctatagcg
      301 cctcgtccgc ctttctccaa attcatggtc actccaagtg aacttttatc ttgacgattt
      361 cactgtgcga tcgtatgttt atggtgttca caaaatacaa acgatccgta ttcgtcctga
      421 ttaatcatca ttaaacgagg aatgttgcat catgagacct aacatttcaa tcactcttac
      481 cacgcctcat gtgactattg aacgctatag cgagctgaca gggctatcca tcgataccat
      541 caatgacatg ttggctgatg gacgccttat ccgtcatcgt ctgcgcaaag ataaaaaacg
      601 cgaaaaagtg atgatcaaca tagcagcaat gaccgttgat gcgctttcag aatgcaatct
      661 aaaccttaat tagttcgatt ctgaaataca tcagaggcat tgaccatgtt tgattaccaa
      721 gtttccaaac atccacattt tgatgaagcc tgtcgtgcat tcgcattgcg ccacaacctg
      781 gtgcaactgg cagaacgtgc tggcatgaat gtgcagattc tgcggaacaa gttgaaccca
      841 gctcaacctc atttattaac cgcaccagaa atctggctgc ttaccgatct gactgaagat
      901 tcaacgctgg tagatggttt tctggctcag attcactgcc tgccatgcgt accgattaat
      961 gaggtggcaa aagagaaact gccacattac gtcatgagtg caaccgcaga gatcgggcgt
     1021 gttgctgcag gtgcggtatc tggcgatgta aaaaccagtg caggtcgtcg tgatgctatc
     1081 agcagcatta actctgtaac acgactgatg gcgttggctg ctgtttcatt gcaggcccgt
     1141 ttacaggcta accctgcgat ggcgagtgca gttgataccg tgactggcct cggtgcttca
     1201 ttcggtttgc tgtgaggtgc ttatgctgac gaaagaacca tcatttgcat cgctgctggt
     1261 aaagcaaagc ccggcaatgc actacggtca cggctggatc atgggtgagg atggaaaacg
     1321 ctggcatccg tgccgttcac aagatgaatt gctggcagaa ctatctacga aaaaacgggg
     1381 gaacaaatgg ctattgaagg cgctgcggcg actgttccat taagccccgg tgaacgcctg
     1441 aatggactta atcacatagc ggaattaagg gcaaaagttt ttggcctgaa tattgagtca
     1501 gagcttgagc ggtttattaa agatatgcgt gatccacggg atatcaataa cgaacaaaat
     1561 aaacgagcac tggcagccat attctttatg gcaaaaattc cggctgaacg tcatagcatc
     1621 agcatcaatg agctgaccac tgacgaaaag cgggagttga ttaaagcaat gaatcatttt
     1681 cgtgcagtgg tgagcttatt tcccagacgg ctaaccatgc cgaattaacc aactaatgaa
     1741 attaatggcg taaacccgcc gagcatccct ttatctaaat tcaggagaat tgattatgcg
     1801 taatattgaa atcctcacga ctaaaaccgg accggatgat gcagggctta atattttact
     1861 gacagaggct cgtctggaag aacgccgggc aagggctgaa gcaatggcag ctcgccttga
     1921 tagcctggcg tgtcatatct catcccgtca gctaaaccac gtggaagcgg cagaactgct
     1981 gcgtgtgact gctgaagcaa tccagaacga agcgcaggag atccactaat ggctgatgca
     2041 atggatctcg tacagcagcg cgttgaagaa gaacgccagc gccatatccg tgctgcccgt
     2101 gccaaaacac cgggcgtgtc tcgcgtgctt tgcattgagt gtgaagcgcc aattccgcca
     2161 gcacgccgcc gtgccattcc gggtgtgcag ctttgcatta cctgccagga aattgcagag
     2221 ctgaaaggca aacattacaa cggaggtgct gtatgagcac catcctgaaa tgggcgggaa
     2281 ataaaaccgc cattatgtcc gaactgaaaa aacaccttcc tgctggcccg cgactggttg
     2341 aacctttcgc gggttcctgt gctgtgatga tggagacgga ttatccctgc tatctggttg
     2401 cggatattaa tcctgattta atcaacctct ataaaaaggt tgccgctgat tgtgaggtgt
     2461 ttatatctcg tgccagagct ttatttgagg acgcaaacag ggaggtgact tattacaaca
     2521 taagacagga gtttaattac tcaactgaaa ttactgattt catgaaagcg gtatatttcc
     2581 tgtatctcaa tcgtcacggt taccgtgggt tatgtcgtta taacaagagc gggcatttca
     2641 acattccata cggtaattat aaaaatccgt atttccctga aaaagaaatt cgcgcatttg
     2701 cagagaaagc ccagcgagca acgtttatct gcgccagctt tgatgaaacg ctggcgatgc
     2761 tgcaggtggg ggatgtggtg tattgtgatc cgccttatga cggtacgttt tccggctatc
     2821 acactgacgg cttcactgaa gatgaccagt atcacctggc atccgttctt gaacatcggt
     2881 catcagaagg acatccggtc attgtttcta acagtgatac atccctgatc cgttcgctgt
     2941 atcgcaattt tactcaccac tacatcaaag caaaacgcag catcggtgta gcagctggtg
     3001 agagtaaatc tgcaacagaa atcatcgctg tttccgggcc gcgctgctgg gtgggatttg
     3061 atccttcgcg tagcgtggat agttctgccg tgtacggagt gcgtgcatga gccatgctga
     3121 tatgaacaac tgcagcggct ttaacgaggt cgccgcagca ttctcatgga acagcccgaa
     3181 aaaggccatt aacccttatc tggacccggc ggaagttgcg ccggtttctg cgctttcaaa
     3241 cctgatcact ctgtacgcta ccgataacga gcaggaacaa ctgcgccgcg aggcactgag
     3301 tgatcaggtc tgggagcgtt atttctttaa tgaatcccgt gatcctgtcc aacgcgaaat
     3361 ggagcaggat aagctcatta gccgggcaaa gatggcgcat gagcagcagc gttttaatcc
     3421 agacatggtc attctgactg acgtcaacgc ccagccttcc catatcagca agccgctgat
     3481 gcaacgtatt gaatatttca gcagcctggg caggccaaag gcttattccc gctatttacg
     3541 tgagacgatt aagcaatgtc tggaacgact agagcatgta cgcgagagtc agctatccac
     3601 ttcttttcgc tttatggcaa gccatgaagg gctggacggc ctgctgatcc tgcctgaaat
     3661 gagtcaggat caggtgaaac gcctgtccac ccttgttgct gcgcatatga gcatgtgtct
     3721 tgatgcagct tgtggcgatt tgtatgccac cgatgacgtt aagccagaag aaatccgcaa
     3781 gacatgggaa agggtggcgg cggaaaccct gcgtctggat gtcattcctc ctgcgtttga
     3841 gcaactccgc cggaaaagaa accgccgtaa acccgtgccc tatgaactca ttccgggttc
     3901 gctggcgcgt atgttgtgcg ccgactggtg gtatcggaaa ttatggaaga tgcgttgcga
     3961 atggcgggaa gagcagttgc gcgctgtctg cctggttagc aaaaaagcat ctccttatgt
     4021 cagctatgaa gccgtgatgc ataaacgtga gcagcgccgt aagtcgctgg agttttttcg
     4081 ttctcatgaa ctggtgaacg aagacggcga cacgctggac atggaggatg tggtaaacgc
     4141 cagcagcagc aaccctgcgc atcgccgcaa tgagatgatg gcctgtgtta aaggcctgga
     4201 gcttatcgcg gaaatgcgcg gtgactgcgc cgttttctac accatcacct gtccgtcacg
     4261 tttccattcc acgctaaata atggcaggcc caacccaact tggacaaatg cgacggtaag
     4321 acaaagcagt gattatctgg tcggcatgtt tgctgcattt cgtaaggcga tgcacaaagc
     4381 cggattgcgg tggtatggcg tgcgggtggc tgagccgcat catgacggta cagttcactg
     4441 gcacctgttg tgttttatgc gcaaaaaaga ccgccgcgcc atcactgcat tactgcgtaa
     4501 gtttgccatc cgtgaagacc gcgaggaact gggcaataac actgggccgc gctttaagtc
     4561 tgagttgatt aacccgcgca aaggtacgcc aacaagctac atcgcgaaat acatcagtaa
     4621 gaacattgac gggcgtggtc tggctggcga gatcagcaag gaaacgggga aatccctgcg
     4681 tgataatgct gaatacgtta atgcctgggc gtctctgcat cgtgttcagc aattccgctt
     4741 ctttggcatt ccggggcgtc aggcttaccg tgaactgcga ttgctggctg gtcaggcggc
     4801 aaggcaacag ggggacaaaa aagcaggtgc gccggtactg gataacccgc gccttgatgc
     4861 catcctggct gctgctgatg ctggttgttt tgccacctac atcatgaagc agggcggcgt
     4921 actggttccc cgcaaatatc acctcatcag aaccgcttat gaaatcaacg aagagccgac
     4981 cgcctatggc gatcacggca ttcgtattta tggcatctgg tcacctattg cagagggcaa
     5041 gatctgcact catgccgtga agtggaaaat ggttcgtaag gccgttgatg ttcaggaggc
     5101 ggcagccgac cagggcgctt gcgccccttg gactcgtggc aataactgtc cccttgctga
     5161 aaatttgaac caacatgaga aagatgaatc agcagatggg gataccagaa cggacattac
     5221 ctgcatggat gacaaggaat tgcacgatta cctgcacagt atgagcaaaa aagagcgccg
     5281 ggaactggca gcaagattgc gcctggttaa accgaaacgg cgtaaagact acaaacagcg
     5341 aattacagag tatcagcgtc agcagctcgt ctatgaactg aagtccagag gatttgatgg
     5401 cagcgagaaa gaggtcgatt tgctcctttg cggtggcagt attccgtcag gagcaggcct
     5461 gcgtatcttc tatcggaacc agcgtttgca ggaagatgat aagtggcgga acctgtatta
     5521 atcacgcggg ttaacaattc gtgctcttaa taataccagg catatcaggc tgatgaacgt
     5581 aaaaaaacgt tttacatcag taagattatt atatactgta aatataaaca gtggttatat
     5641 gtacagtatt gcgtatggtg ttataggagg aaagatgcag gactattttt tggagtcttt
     5701 gaagctccag cgcattgatt tttttcttaa gcttgtagcg gctagtgagt gtagtgatga
     5761 agagaagggg ctagctctgc agtgggtttc tgaattgact gatgagttga tggcgaaaat
     5821 tcgaagccat gaatacagta ggtcgatgga tgtaatcagt tgagcttgtt tcgttaggac
     5881 ttgataaggg aaccgaaaag gggcattaag cccctttctc ataagttgat ttagttgtta
     5941 cccaaagagc cggccatggc tgcatattta atttcttcgc tgggtatttc aggatgtagt
     6001 tgaatttgcc tgatgatata gccaggaact tcttcactcc acagtttcgc cgcgtcctta
     6061 cagaccttat acgcgaagtc tctattctca aaggcatagg attttccgta tgcatattgc
     6121 tgacaggcat gaacataagc tccgaagtcg ccgtctttat ctggcaaagc acatccagct
     6181 aaggctatga tgattgctac taacgtaaag gagaaagtta tacgtttcat ggtctctttc
     6241 taagcgttta gcattattta actaaggggt atctgccgtg atttggtagt ccctgatata
     6301 tattctcagg gactatgctg aagtagcttt aattttgatt atttagaaag gtacttatct
     6361 ctaagttgat tcagtctgtc agcatcgcgt tctgaaaact cggcgtcacc atagatcgat
     6421 ttcccatctg cgtcttcaag gccaatgact gtgacggtaa atatagcatc agccggaact
     6481 ttaagttttc cccaatcaga gtatctatta ggggctaatt tccagttggc tttctcacct
     6541 ggttccagac ctccagaaat tttatagttg aagacgtcag agaaccaagg gacgcttctt
     6601 cctgggctgg caataactcc tttgaagaat actctggcaa ccgctttgtc tgttccgttt
     6661 tcaacgctaa tatccagaat tggctgatca ttgccgtaat cctctttctg gaaataaaaa
     6721 cgggaacgtt cgaccttgaa ggctttcatt ttttccgcag cctgtgttgc agaggtttgt
     6781 ttggcttcca gttcttttat ttcttgcaga gcctgttctt tctctctctt ctcacgctca
     6841 aggcgaatag tttgtgcgta atttataacc tcatcaccag ttttaccttc cagcgctgac
     6901 ttaatttttg tttcgtagac gtccccagaa gacgttccag cctgcattag ctctctcatg
     6961 ttgatttggc taaaggcaac gactttaact gcatcatcaa actgagcttt tttatctgca
     7021 ggtaaggact ctcttacttt ttgaatggac tctttcatcg actgatcgct tgatgcatcg
     7081 atcttcggtt tgtcacaagc agtaagcagc agagcaaaag caattacgcc agttaatttc
     7141 ttcatatccc tatcccaatc gtaaaaaaac aaggctaatc ctaacaggat tagcgggcat
     7201 gacaaactca tagccatata ggtgtgctgt ataaattagc gggatgagaa agcctgcaca
     7261 gaccctcatg ttgaaacttg attttagctc aaaagagctg acactaaata actgcgcgga
     7321 ataatgctcc actttcggtt agcccttggc ccttgctggg taaggcgtgg ggccgttttc
     7381 ttcgcctgat cctgctgtat ctggcctaca gtatgttgtc cttacgcgac atgccacatg
     7441 tcagtaaacg gcaggtgtag cagtattaga cgcagctatt tagtgtgtat taagctggct
     7501 catatttatg actggtttca aggtttgacc gattttcatg tcatgcatgt ctatgctgca
     7561 tgagttcgca tgatcgtttg aggatcggtt tggctaagac atgccagaac tggcgagctt
     7621 ttgctcatgt catgcaggtg catgaaaact actacataaa gcgggcaggc gtggcgggga
     7681 tacgagcgcg cgctgtgctg tgtggtatag gccttaattt agtatgattc aatagtgtcg
     7741 ggagctttgc gctgatcagt cctaagttat atgataagaa aaatgcataa aaaaggttcg
     7801 gttcatgggg ttaggattaa aagacgctat tgtgcatacc gctgcgtttc atcaaaagga
     7861 aactaatgaa ttgcaattgc caaacaatca ttggcatcct ggttttatca ctgtcttagc
     7921 agcttacgtg aatcatcaca aaattaccga agataactgt gttttgtcat gccctggcta
     7981 tatgcgagct atcaatttac atggtgcttt atggggagag gatcagtatc aacaggaacg
     8041 tgtaaatgtt ggaagaaatt acagtttggt tacagcatta accaatgttg aagctgttga
     8101 cgttgccact ggtagtatta acagttgtgt aaggcagtta acattccctg atcgtgatcc
     8161 gagagattgt cccaagggac ttacagatct aacccatgtt ataggtgagc ttcacgataa
     8221 cgtttggtcg catggaaaat caacgggttt ttcttttgca caacgttcgg ctgtacccca
     8281 cactcaaaaa aaagagcact atttagaatt ttctttggcg gactgtgggt taggatttct
     8341 ccgagaattg cggcgagctg gtatacccgg aattgagact caccaggatg cgatcgcatg
     8401 gtgtattcaa gagggacatt catctaagca tgctgatttg caggatgatt gggcacagca
     8461 gcttccccag gactttattg ggggtagtgt gtttggccac ggggtttcgg tcaaagaaaa
     8521 agataataat catcaagggc ttgggttgta tcacttgatg aaattgataa aaacatataa
     8581 tggggaattg caactggctt caggaaatgt atgcttagaa gcaataggtg atgaagtgag
     8641 ctatactgaa ttacgtactg aatggccggg tgtagcaatt tcttgccgtt ttaagattca
     8701 tgaactggca gtcgataaag atagcgaaga aaatgaccct cagcttatgg aaataatgcg
     8761 ggcgttagga ggagagtaat gaacaaaatc gcatacaagt tacccgaggg tgacctggct
     8821 tcgcgcaatc aggctatccc ccaacgacac aagattgaag ggttcattaa agaagggtat
     8881 accgtagatt tagatctgag cggtgtttat tcaatttcag aatcatactc tgatgaaatc
     8941 tttggtgttt tggtagtgaa attcggtgta accaaagttt tgagtcaagt taaggttcgc
     9001 aatgcacccc cttcaatttt aaaaagtatt gcaaaagtga tccaacgccg tagtaatgaa
     9061 gtagcatcaa aaaaggttca ttccgttgga tttgatggtt tatacgccgt ttgttaatat
     9121 atggttagtt agataaaagg cgctgagatt agcgcctttt cttttattcc tgaacaccta
     9181 aaacatagtc atcaaaacgt attacttctg cacccaccca ttcgtttagt tcatgtagtc
     9241 gcttctgtag tggcatcaat tcattgcgaa caaatacaag actagccttc tccacatccc
     9301 caaaaccccc aacattattt ggcataatcc ccatcatctg tggcggaacg cgatgtgctg
     9361 ccatcatgtc atcgcggctg acatttttga tattcagaaa ctcatccttc gccgcaactt
     9421 ctgacaaagg gatgatctga aggccgtcct ttttgccgtt aggcgagtac ataaacaggt
     9481 tgcggaagtt gcctggacct ttggcgcttt tcatcgcgtt gcggagattg ttcacatcct
     9541 cctgattctg cgcggcgtcg gtcatgtaca tgatgaagcc tgcatggctg ccgttgatgt
     9601 aatacttacg gcggaacagc gtggcggact cattgagcag ggctgacgga atggcagaaa
     9661 gatagccggg caggccgtag atctcctggt taatgtccgg ttccatcaga tgaaagatgc
     9721 tgcctttcgt gaactgatac ggctgtgttg tcataccgta ttgcacaaac cagtaggtat
     9781 ccaggtctaa cccgcgtcgg gtgtattttg ccagggccgg ttcaagggcg ataacttcac
     9841 cgaagcggtt cgtgcgtttc tccaggtagg cgttaccaaa taccagatag tcctgcacaa
     9901 aacgtgaaaa agcctgctgg ctgagcagcg ggtgagggat gtaggtactg gtcagaatgt
     9961 tgcactttac tgcaatcggg gaactgtgat gcacagcagc gcggaaggtg cgcgccagtc
    10021 cgtcaaagct gacaggaggc tcataccagc gatctgtctg tacgcattcc acatagtcca
    10081 gcagttcgcg gcggtcaagt actggaatgg gatcaccgaa gctgaatgct tcggctgtag
    10141 tctggctttt aagctggctc tgtttcgtcg ccgcagcgcg gttcttctta ctctttccca
    10201 tcaaaaaatc tccacaatat tgctggtatt ggcggactcg ccctgcagcg gttcgttaaa
    10261 gagtgcgtgc attgttgccc aggccagatc ggcatggctg gcttcttcgc tgcggctggc
    10321 ttcataggtc gggcggttgc cgctggcggt ggtggcgcga cggattgcca tgaatgactg
    10381 cgcaatgtcg gtgtgtccgg cgtcaaattc taggcggcgg tgactgataa tgtcgtaggc
    10441 cttgagtacc agggcgtttt taacgttggg gttgtagaca aactcccgga cggcaggaaa
    10501 gaatgctttc acgttctcgt aaaccccgtg gccgacgcca gttgagtcga tgccgatgta
    10561 tgtcacgttg tactgttcgg tcagtttttt gatggcgtca gcctgggcgc ggaagtccat
    10621 cccgcgccac tggtgacgct caagaatgcg gaacttaccg cccggcacgg ctggcggtgc
    10681 caccactacg catccggcgc tgtcgccgtt ttgcgtacct tttgccgggt cataaccgat
    10741 ccacacttcg cgccagccaa aggggcgcag agccagtgca tgaaagtcgg tccagacttc
    10801 ccagctgtcc accatgcacg cctgcagctc gctgagcggg aacacggacg cgagatcgtc
    10861 cacgaactcg cacatcagca ggttctggta ttcgtccggg ctgtactcca tgcgcaactg
    10921 gtcaaggtcg aacaggttac agccgccgcg caccgcatct tccacggtga ctatctggcg
    10981 gtattgcccg tctgcgcaca gcaggccggg ggccagattg ctgtgggaca ggtcgatgtc
    11041 caccttatcg gctttgttgc gcccacggtt gaacagcgca ccggaccaga acggataagc
    11101 actgtgtgtc aggctggatg gcgtggaaaa ataggtttgt cgccattttt tgtgaatagc
    11161 cataccggaa gccactttgc gcagctcctg gaattttggt atccagaaat attcatccag
    11221 atacaggttg ccgtgataac tctgggcagt gcgggcattg gtgccgagga agtaaagcgt
    11281 ggccccgttg ggaagcacca tcggatcgcc tttcagctcc acctccactt ctttggcgaa
    11341 gtcgatgatg tactgtttaa agacgtgggc ctgtgcctta ctggcggaaa ggaaaatctg
    11401 gttacgtccg gtaagcaggg cgtcaatcag ggcttcacgg gcaaagtaaa aggtcgcgcc
    11461 gatctggcgt gacttcagca ggttgcggat gcggttggtt tttccggctt cccaccagtg
    11521 gcgctggtag ttgaacatgg aggaatggaa aatttcttcc agcttctcaa tctgttcatc
    11581 ggtgaaaacg ttcttttccg gctgacggcg cgggcttttg ttgcggttgg cgacgttagg
    11641 gtttaagtcg gcttcgttgc cgccattgtt aaacttgccg atccgcgcgt ggcgttccga
    11701 ctggcgcgcc agcaggtcaa tctctttgaa atctttccct tctttgtgct ccttcatgat
    11761 gagctggcag tagcgtgcgg cggtggtgag ctgcatctga tccagcggcc cataatcacc
    11821 ccacttgtcg cgttttttcc agctgtgaac ggttgcaact ttctcgccca gcatttcagc
    11881 aatgcgggct acgcggtatc cctgaaagta cagcagcatg gcctgccgac ggggatcgag
    11941 atctgcgggt gtcagtgtgg tgttcatggc acaaacctac agccttgaat gaaggctttc
    12001 cccgcctgcg gtttgtgtgg ttgtcggtac aaataccgcg cattgtttca ctgcccccat
    12061 caccgcaacc ataaggctcc agtaagtttt ttctaacgga gcacggctca tgacagtgaa
    12121 agcaaagcgt tttcgcatcg gggtggaagg tgccaccacc gacggacgcg aaatccagcg
    12181 tgaatggctg gaacagatgg cagccagcta caacccggcg gtgtataccg cgctgattaa
    12241 ccttgagcac atcaagtctt atctgccgga cagcaccttt aaccgctacg gcaaggtgac
    12301 ggcgctgttt gctgaagaaa tcacggaagg cccgctggca ggcaagatgg cgctgtatgc
    12361 cgacgttgag ccaacggagt ccctggtgga actggtgaaa aaaggacaga aattattcac
    12421 ctctatggaa gtcagcccga agtttgctga tacgggcaaa gcctacctgg tcggcctggc
    12481 tgccactgat gatcccgcca gtctgggtac ggaaatgctg acattcagcg ccagtgcagc
    12541 ccataacccg ctggcaaacc gcaagcagaa tcctgccaat ctctttaccg ctgcagagga
    12601 aacggtgatc gaactggaag aaatccagga cgacaaaccg tccctgtttg cccgcgtcac
    12661 ggcgctgttt accaaaaaag agcagtccga tgacgcccgg ttctctgatg tgcataaggc
    12721 cgtggagctg gtcgccactg agcagcagaa cctgagcgca cgcaccgaaa aatccctgtc
    12781 tgagcaggaa gaacgcctgt ctgagctgga gactgccctg caggcacagc aaaccgcctt
    12841 taacgaactg gtggacaagc tgagtcatga agacagccgc caggactacc gccagcgtgc
    12901 aacaggcggt aacgcccccg ctgacactct gaccaattgc tgatggagca caaaacccga
    12961 tgaagaagaa tacccgcttt gcttttaacg cttacctgca gcagctggcg cgtctgaacg
    13021 gtgtggcagt tgaagaactg tccagcaagt tcactgtaga gccgtctgtg cagcagacgc
    13081 tggaagacca gatccagcag tccgccgctt tcctgacgct gattaacgtc acgccagtga
    13141 ctgagcagtc cggtcagctg ctggggatgg gtgttggcag caccattgcc ggaaccactg
    13201 acaccaccgc gaaagagcgt gaacctgtcg atccgacgct gatggtcgat gtggaataca
    13261 aatgcgaaca gaccaacttt gacaccgtgc tgacctacgc gaagctggac ctgtgggcga
    13321 agtttcagga tttccaggtg cgtatccgta acgccatcgt gaaacgtcag gcactggacc
    13381 gcatcatgat cggctttaac ggcgtgaagc gtgcgaaaac ctccaaccgt agcgaaaacc
    13441 cgctactgca ggatgtgaat aaaggctggc tgcagaaaat ccgtgaggat gcaccggatc
    13501 acgtcatggg cagcaccacc acgggcggtg aaaccacacc gggtgcggtg aaagtcggga
    13561 aaggtggcga atatgccaac ctggacgccg tggtgatgga tgccgttaac gagcttatcg
    13621 acgtggtcta tcaggacgat gacgatctgg ttgtgatttg cggtcgtgaa ctgctgtctg
    13681 acaagtattt cccgctggtc aacaaagagc aggaaaacag tgaaaaactg gctgccgata
    13741 tgatcatcag tcagaaacgc atgggtggcc tgcaggccgt gcgtgcgccg ttcttcccgc
    13801 cgaatgcgct gctgatcacc cgtctggata acctgtccat ctactggcag gaagacaccc
    13861 gccgtcgttc ggttatcgac aacccgaaac gtgaccggat tgaaaacttt gaatccgtta
    13921 atgaagccta tgtggttgag gactaccgct gcgccgcact ggtggaaaac atccagattg
    13981 gtgatttcag cgccgccgca gccgaaaccg gagcgtaatc catgagcctg agtcccgcac
    14041 ggcagcatcg cctgcgcgtt caggctgaac aggccgcccg cgagggcggc agtgttcgcc
    14101 acgcatcggg ctatgacctg atgctgctgc aactggcgga agaccgccgc cgtctcaagg
    14161 gcgttcagtc cacggtcaaa aaagcggaaa tcaaggtgga gctgctgccg aagtacgccg
    14221 cctgggcaga gggtgtcctg gctgccggag gcgctcaaca ggatgacgtg ctgatgtacg
    14281 tgatgctgtg gcgcattgat gccggagatt atgccggagc gctggagatc gggcgtcatg
    14341 ccctgcgtca tggctgggtg atgccgctgg gtaaccgcaa cgtgcagacc gtgctggcag
    14401 aggaaatggc agacgccgcg cagagcgcaa tgcttgccac caccggcttt gatgccgatc
    14461 tgttgctgca gacgctggag ctgacagacg gtctggatat gccggaccag tcacgggcgc
    14521 gtctgcataa agcgattggc gcggtcctga gtgaaagcaa tccggcgtct gcccttaatc
    14581 atctcaacca tgcgttacag ctcgatcccc gctgtggcgt gaaaaaagac aaacagcagc
    14641 tggagcgcag actgcgcaat gacagccgct gacagaacgt gcccccgcgc acgggcggca
    14701 cggggtggcg aaaggcactg ccacatcaaa accccgtcca ccgccctcta tttcaggaga
    14761 aagcagcatg aagtttgttg cgccagaaca ggcaccggaa caggcggaaa tcatcagaaa
    14821 tacgccgttc tggcctgatg tggacctgtc ggagtttcgc agtgtcatgc gcactgacgg
    14881 cacggtgacg cagccgcgtt taaagcaggt tgcgctgtcg gcaatttcgg aggtcaacgc
    14941 agagctgtat gagtttcgca gacgccagca gatgctgggg tatgcctcgc tggcagaggt
    15001 tccggcggaa cagctggacg gcaaaagtga gcgcattcag cactatttca acgcggttta
    15061 ctgctgggca cgcgccatgc tcaacgaacg ttaccaggac tatgacgcca cggcatccgg
    15121 tgtgaagcgg ggcgaggaac tggcggaagc cagcggtgat ttatggcgtg acgcccgctg
    15181 ggccatcagc cgggtacagg acgcgccgca ctgcacagtg gagcttatct gatgaaagtg
    15241 cgtgcgcatc agtatgacac ggtggacgcg ctttgctggc gtcattacgg gcgcacgcag
    15301 ggtgtcacgg agcaggtact gaaggcaaat ccggggcttg ccgaatatgg ccccttttta
    15361 cctcacgggc tgcaggtgga gctgccggac attccgacaa ccaccaccgt gcagaccgtc
    15421 cagctatggg actgaattat gacgcttgag cgaatcagcg cctttatcac gtactgcatc
    15481 gccgttgtgc tggcctggct gggcgatttg tccatcaagg atgcctcaac gctgggcggc
    15541 ctgatgattg gtgtgctgat gctggctatc aactggtact acaaacacaa agcctaccag
    15601 cttctgcgcg acgggcagat ctcgcgggag gactatgaat ccatcaatcg ttaaacgctg
    15661 ccttgtcggg accgtgctgg ctattgctgc cacgctgccg ggttttcagc agcttcacac
    15721 ctccgtggag ggactgaaac tgattgccga ttacgaaggc tgtcgtctgc agccgtatca
    15781 gtgcagcgcg ggtgtatgga ctgacggcat tggtaatacg tcgggcgtca ttcccggcaa
    15841 aaccattacg gaacgacagg cagcagaagg gcttatctcc aacgtgctgc gtgtggagcg
    15901 ggcgctggaa agatgtgtga agcaacagcc gccacaaaag gtgtatgact cggtggtgtc
    15961 gtttgccttc aacgtgggga caggcaatgc ctgcagctcc acgctggtga aattgctcaa
    16021 tcagcggcgc tgggcggatg cgtgccgaca gttgccgcgc tgggtttatg taaaaggtgt
    16081 gtttaatcag gggctggata accgccgtgc gcgggagatg gcctggtgtt tacagggagc
    16141 aaactgaaat gaaaaagaaa ttaatcagcg gactgtttct gatgttatgg atggcgctgt
    16201 taatcgcagc aatggtgtat ccgcagggga tctttccggt actggcagcg tccggcgttt
    16261 gggtagcctg tttgctgaca tgggcggtaa ttccggtagc actggctgcg ttaattcaga
    16321 atggcccgct ctggcaggag ttaagggcat ctttgctgaa gacaattacc cgaaaagaaa
    16381 acgtatttat cagttgggtg atgcgattgc tgattgttgt aagtctcgca tggacggggt
    16441 gggctattac cctggtcttt tatctactga ccgttattgc cttctggatc acccgtaatc
    16501 agatggcgca acaggtagca gcatgaaccg gttgctgctg gttgtgctgg cgttattact
    16561 ggcggctctg ggctggcaga cgtggcggct ggctgatgcc agccagacca ttagcacgca
    16621 ggcagacgag ctgcagagca aaagccaggc actggcaaag agcaacagcc agcttatcag
    16681 cctgtccatt ctgactgaaa ccaataaccg ggagcaggcg cggctctatg ccgaagcaga
    16741 acagaccagc gcactgctga gacaacgaca acgccggatt gagaaactga aacgtgagaa
    16801 cgaggattta cgccgctggg ctgatactcc tttgcctgct gacattatcc ggctgcggga
    16861 acgtccgacg ctcaccggag gtgcagctta ccgtcagtgg ttgtccgcga gtgacgccgt
    16921 gtcggctgga gcaggcagca ccgcgcacta acggtgatct gaatgcgttg ctggatgaaa
    16981 cggaggccgc ctgggcggtc tgtgcagaca aagtggacat gattattgcg tgtcaggagc
    17041 gaaacagtga acaaaccaca atccctgcgc cacgccctca ataaagcggt gccttatgtt
    17101 cgcaataacc cggacaaact gcatctgttt gtggataacg gttcgctggt tgccacgggg
    17161 gccagctcca tgtcatggga gtaccgctac accctgaacg tggtgattga ggatttcagc
    17221 ggcgaccaga atctgctgat ggccccggtt ttgctgtggc tgcgtgataa ccagcccgat
    17281 gccatcaata acccggcgtt acgggaaaag ctattcacct ttgaggtgga tattctgcgc
    17341 aacgatgtct gtgatatcag cctcaacctg caactgacgg agcgtgtgct ggtcagcact
    17401 gacggcagtg tgtcgagcgt tgaagctata gcggaacctg atgaacctga agaaatgtgg
    17461 acggtgaaac gtggctgaac tgcagaaagt ggacgactgg ctgagtgcct tgctggcgaa
    17521 tctggagcca gccgcaagaa gccacatgat gcgccagctg gcgcaggaac tacgccggac
    17581 acagcagcaa aatatcagaa tgcagcgcaa tccagatggc agcagttatg aaccgcgacg
    17641 ggtaacagca cgcagtaaaa aaggccgtat caaacgtcag atgtttgcaa agctgcgcac
    17701 cacaaaatac ctgaaaactt ccgccagcgc cgactctgcc agcgtgcagt ttgaaggcaa
    17761 ggtgcagcgc attgcccgcg ttcaccatta cggcctgcga gatcgcgtca gtcgtaaggg
    17821 accggaggtg cgttacgcag agcgtcgcct tctgggtgta aatgatgatg ttgaggcaat
    17881 gacccgcgac atgattctgc aatggctggc ggggtgatct ttgtatcagc actgatacaa
    17941 gttgcagcac tgccgccttt cttcccctga tggcaacctt tccctatgaa cgcacaatta
    18001 accgaaatca tgcgccttat caccaacctg atccgcacag gggtagtcac cgaagtggac
    18061 agggaaaact ggctttgccg ggtgaaaacg ggcgagcttg aaaccaactg gatcagctgg
    18121 ctgacgctgc gtgccgggaa tgcccgtaca tggtggcgac catcggaagg tgagcaggtg
    18181 gtgctgctga gtctgggcgg caatctggaa accgcctttg cgctgcccgc tgtctattcg
    18241 aatcagttcg caccaccgtc gacgtcggcg gacgcctgcg tgacagaaca tcctgacggt
    18301 ggctggtttg aatacgaacc cgccaccggg cgctggtatg tcaggggcat caaatcaatg
    18361 gtcattgagg ctgctgacaa catcaccatg aaaaccagtg agtttgtact ggaggctgac
    18421 cgcacgcgca ttaacagcga agtggtgatc aatggtggcg ttacccaggg cggcggagcg
    18481 atgagttcta acgggatcgt ggttgatgcg catcagcata ctggcgtcct gaaaggcggc
    18541 gatacaaccg gaggcccggt atgacgcttt atagcgggat gaacaatacc agcggcaaag
    18601 tcattactga tattgatcat ctgcgccagt cggtgcggga cattctgctg acaccgcagg
    18661 gtagccgtat tgcccgccgg gaatatggtt ccctgctgtc ggcactgata gatcagccac
    18721 aaaatccggc attacgcctg caggtcatgt cggcagtgta tgtggcgctg agtcgctggg
    18781 agccacggtt gacgctggat tccatcacca tcaacagcaa ttttgacggt tcaatggtgg
    18841 tggagctgac cgggcggcgg aataacggtg tgcctgtgtc cctttccgta tcaacaggag
    18901 cagagaatgg cagtgattga cctttcgcag ttgcctgcac cgcagattgt ggatgtgccg
    18961 gactttgaga cgctgcttgc cgaacgcaag gcagaatttg tggcgcttca tccgaaagat
    19021 gagcaggaag cagtgatccg cacgctggaa ctggaatctg aacccgtcac taaattgttg
    19081 caggagaacg cttaccgtga gttgcttctg cgccagcgca ttaacgaagc cgcgcaggcg
    19141 gtgatggtgg cttacgcgat gggcggcgat cttgaccagc tcgctgccaa ctacaacgtg
    19201 acacgcctga cggtgacgcc tgctgataat gatgctgtgc cgcccgttgc agctgtgatg
    19261 gaaagcgatg aagcgttgcg cctgcgtgtg cctgcagcct ttgaagggct ttctgttgcg
    19321 gggccaactg cagcttatga atttcatgcc cgaagcgccg acggtcgggt ggcggatgcc
    19381 agtgcaacca gcccggcacc tgcagaggtg gtgctgactg tccttagccg cgaaggcgat
    19441 ggaactgcag aaaaagacct gctggacgtg gtggaaaaag ctctgaacag tgagaacgtc
    19501 cgcccggtgg ctgaccgtct tacggttcgc agcgcagaaa tcatcccgta tcgcgtggaa
    19561 gccaccattt ttctctatcc tggaccggaa gcagagccgg taatggcagc ggcaaaagcc
    19621 agcctgcaga agtacatcgc cagtcagacg cgtcttggtc gggatattcg ccgtagcgcc
    19681 atctttgccg cactgcatgt tgagggggtg cagcgtgtgg agctggcttc tcctctggcg
    19741 gatgtggtcc tgaacaaaac acaggcggca tcatgtacgc agtggagcgt aaccaacgga
    19801 ggaacggatg aatagtctgc tgccaccggg ttcaacacca ctggagcgcc gactggcgca
    19861 aacctgcagc gggatttctg atctgcaggt gccgcttcgt gatttgtgga atccggcaac
    19921 ctgtccggtc agtttcctgc cttatctcgc ctgggcgttc tctgtggatc gctgggacga
    19981 gggctggaca gaaagcgtca agcgccaggt ggtgaaggat gctttttata ttcatcagca
    20041 taaagggacc accagtgccg tgcggcgggt ggtggagccg ttcggattcc tgatccgcat
    20101 tattgagtgg tggcagaccg gagaaacacc gggcacgttt cgcctggata tcggcgtgca
    20161 ggaccagggc atcactgaag atacctatct ggaacttgag cgactgataa gcgatgccaa
    20221 accatgtagc cgccacatga tcggcatgtc catcaatctg cagaccagcg gcccgcattg
    20281 ggtgggagcc gccagctatc ttggcgaaga aatcacgatc tatccgtata tcaacgaaac
    20341 gattatttcc ggtggcaccg cgcatgaagg cggggcggtc catgttattg acacaatgag
    20401 agtgaatcca tgagcacaaa attttatacc ctgctgacgg atattggcgc ggcgaaactt
    20461 gccagcgccg ccgcgctcgg tgtgccgcta aaaattaccc atatggcggt gggcgatggc
    20521 ggtggagtat tgccaacgcc ggacgcaaag cagacggcac tggtaaatga gaaacgccgg
    20581 gctgcgctga atatgcttta tatcgacccg cagaacagca gccagattat tgccgaacag
    20641 gtgatccctg aaaacgaggg cggttggtgg atacgtgaag tgggcctgtt tgatgagtca
    20701 ggggcattga ttgccgtggg caactgcccg gaaagctata agccgcaact ggctgaaggt
    20761 agcgggcgca ctcagaccgt gcgcatggtg ctgattacca gcagcacgga caatatcacc
    20821 ctgaaaatcg accctgctgt agtgctggca acccgcaagt atgtggatga caaggcactg
    20881 gagctgaagg tgtacgcgga tgatcagatg gcaaaacatc ttgccgcacc ggacccgcat
    20941 tcacagtacg cgccaaaagc cagcccgaca tttaccggaa cccccaaagc gccaacgcca
    21001 gcggcgggga ataataccac gcaggttgcg accactgcgt ttgtacaggc ggcactgacg
    21061 gcccttatta atggtgcgcc agccacgctg gacacgctga aagaaatagc cgcagccatt
    21121 aacaatgatc cgaatttcag taccaccatt aacaatgcgc tggcactaaa agcaccgttg
    21181 tcgagtccgg cactcaccgg aacgccaaca gcccccacgg cggcgcagtc ggtcaacaat
    21241 acacagattg ccactacggc ttttgtgaaa tcggcgattg caggaatggt gggttctgca
    21301 cctgctgcac tggatacact gaacgaactg gcggcggcac tggggaatga tccgaacttt
    21361 gccacgacaa tgcttaatgc gctggcaggt aaacaaccgc tggacaatac gcttaccaat
    21421 ttgagtggaa aggatgtagc tggtcttctc acataccttg gtttgggaga agcggcgaaa
    21481 aggaatgtgg ggactggggc gaatcagata cctgatatgt catcatatgc atcaggttca
    21541 ggctggcgaa aaatgccaga tggttcaatt gaacaatggg gaggaattcg ttttcctggt
    21601 gaacacgagc ctgtatccgc caatgtttca ttcccaattc catttacaca gacaccgaac
    21661 attataattg tgttcgatgg tggtttcggg ggcgggaata tgtggggggc gaccaactgg
    21721 agcactaccg gcttcatagc tcactgcaat tatggtcttg aaggtggtgt atttttcgct
    21781 aagggctggt aatgatgaaa tatctgtacg taaacaacct ggcatatcct tacgaacttc
    21841 agtctcttta tgttgaaaaa ggtgaatggc cggaagaaaa aggtgttgat atagacgaag
    21901 taattttcag ggaatatttc tatgatacac caccagaagg aaaatacaga tgtgtcggag
    21961 aggatggact gcctgcatgg gcagatattc ctccaccaac acgtgaagaa caaattgcat
    22021 cagccgaaac taaaaagcag caattgatta accaggccaa cgaatacatc aacagtaaac
    22081 aatggccagg taaagcatca attggtcgtc tgaaaggtga ggaactggtg caatataatt
    22141 tgtggctgga ttatctggac gcactggaac tggtcgatac ttccggtgcg ccagatattg
    22201 aatggcctac gcctccggca gttcaggcca gatgacgtcc ggcgctgtgc tggtatctgt
    22261 tgccgtcacc gcgtcaatgt aatccagcac agcgttaagt cgggttgttt ctgcctgcgt
    22321 caacttccgt ccggcctgta atttcagctg aatcagacta atggaagcca ttgctgcatt
    22381 aatcagtgac tggcgctgtg cttctgccgc gtctagtgcg gcgctatgct gtgcctcggt
    22441 atccgtcatc catttctcac catcccattt atcgtatggc gttaatgggg cgatagtggt
    22501 tgtattttca gggtaatcac ccggaactgt gatttctttg gtatctcccg tttcggtact
    22561 atagacaact tcaccgcgat ggtctggcac atattcccat gaatttaaat ctacagagcg
    22621 acagattgca taaccagctt tatgtgtacc tggtgcatcc agacaggaac atgccgggat
    22681 accgacgccg acagcgagat attcagttga tgtagaaata tattcccgcg tttcgccatc
    22741 atagttatgt acgatgattt ccccatcatt taaagcgtaa ccgtcttttc ccaaaatagc
    22801 ctgttccatt tatgcagccc tcacaatgta gttaaatgca atgtttcgcg ggctaccatc
    22861 accggcgccg gaactgacat gattggttct atccattcgt ttactgtcat ttgttgttcc
    22921 actcccatcc ttccacgaat acaatatccc gccatcgcca atgctcaact gcaccgcaaa
    22981 tacggatgat gtacctaatt caccaacatc tctagccatg agtgggttcc ctgtccatcc
    23041 atgcatttca tggttatgtt caaggataga tgctgcctga cttgaaagca gcagtctcgc
    23101 ggaatcaata cctcgcccat catcccaccc gcgaataaac tcaccgcgca aatccggtaa
    23161 ttttaacttt gggtaagcct ttgccagttt tgggtactct tcggcagaaa aaggtgcacc
    23221 gttgcatttc agccagcctg ttggtggtgt ggctgaaggc cacggaacag gcacaccaac
    23281 gggtaacgct gagccttctc ccaaaccaac gtttaagaaa atgcagaggt aacagctaac
    23341 tggcatcatc tccggttttt attcaggggg atgatcatgc ttattggcta tgtacgcgtg
    23401 tcaacaaatg accagaacac cgatttgcaa cgtaatgcac tgaactgcgc gggatgtgag
    23461 cagatttttg aggacaaaat cagtggcact aagtccgaca gaccggggct gaaaaaacta
    23521 ctcaggacac tatcggcagg agacacgctg gttgtctgga agctggacag gttggggcgc
    23581 agtatgcggc atcttgttac gctgatagaa gagttgcgcc agcgtggtgt gaatttcaga
    23641 agcctgactg acagtattga taccagtacc ccaatgggcc gtttcttttt tcatgtcatg
    23701 ggtgccctgg ctgaaatgga acgcgaactg atagttgaac gtaccagggc agggctggct
    23761 gcagctcgtg ccaaaggcag agtaggtgga cgccgtccta agttgaccac cgaacagtgg
    23821 gcacagattg ggcgtttact cgaggccgga gaatcaagac agcgtattgc actgattttt
    23881 gatgtaggcg tttctaccat ttatagaaaa tttccggcaa ataagatcaa tgaatccccc
    23941 tgaatcagca ttatgttgat tatccctgca agcagacaaa taccgttatt ttgtgtgaat
    24001 aacgacacaa ctgcgcttag ctgtttgtca ggcacaatca cttcaacata gggcgaagcc
    24061 taatccaatc aggaggttcg ccactatggc tcaggattac caccacgggg tgcgcgttgt
    24121 tgaagtcaac gaaggcaccc gatctattac cacggtgagc accgccatcg tgggtatggt
    24181 ctgcacgggc gatgatgccg atgcaaaaat gtttcctctt aataaacccg tgctgatcac
    24241 tgatgtgctg actgccagcg gtaaagcggg tgagtcaggt actctggccc gttcgctgga
    24301 tgccatcgct gaccaggcaa aacccgtgac cattgttgtg cgtgtgccgc agggtgaaac
    24361 ggaagacgaa accacgacca atatcatcgg cgcagtgact gctgaaggta aaaaaacagg
    24421 tatgaaagcc ctgttatctg cccagtcaca gctcggcgtt aaaccgcgca ttctcggcgt
    24481 gccaggccac gacaccaagg cggtagctac tgagttgctg agcgtggcgc aaagcctgcg
    24541 tggatttgct tacctgtcag cgtatggctg caagacagtg caggaggcga tcacttaccg
    24601 cgaaaacttc agccagcgcg aagggatgct gatctggcct gactttactg gctgggacac
    24661 ggtgctgaat gccgaagcaa cggcatatgc caccgcccgt gcgcttggtc tgcgcgccaa
    24721 aattgacgag cagaccggat ggcacaaaag cctgtccaac gtgggcgtga acggtgtcac
    24781 cggaatttct gcagatgtgt tctgggatct gcaggacccg gcaaccgatg caggtctgct
    24841 gaaccagaac gacgtcacca cgcttgtgcg taaagacggt ttccgcttct ggggttcccg
    24901 ctgcctgagt gatgacccgc tctttgcctt cgaaaactac acccgcacgg cgcaggtgct
    24961 gatggacacg atggcagaag cacacatgtg ggcggtggat aaaccgctta acccgtcgct
    25021 ggcccgcgac attatcgaag gtatccgcgc caaaatgcgc agcctggtca gtcagggcta
    25081 tctcattggt ggtgattgct ggctggatga gtcggtgaac gacaaagaca cgctgaaagc
    25141 cggaaaactc accatcgact acgactacac gccagtgccg ccacttgaaa acctgatgct
    25201 gcgtcagcgc atcaccgatc agtacctggt taatttcgcc agccaggtca gcgcgtaagg
    25261 ggacaacatg gctttaccac gcaaattaaa acacctgaac ctgtttaacg acgggaacaa
    25321 ctggcagggg atcgttgagt cgctgacgct gccgaaattt acccgcaaat atgagaagta
    25381 tcgcggcggc ggaatgccgg gtgcggtgga tgtggatctg gggcttgatg acagtgcgct
    25441 ggacacagaa ttttccattg gtggtactga attgctgctg tttaaacaga tgggtaaagc
    25501 cacggtggat ggcatccagc tgcgctttac cggctctatt cagcgtgacg ataccgggga
    25561 agtgcaggcc gtggagcttg tcgtgcgtgg acgtcacaaa gaagtggatt ccggcgagtg
    25621 gaagacgggc gaaagcaaca ccaccaaagt gaccagtacc aacagctacg cgaagctgac
    25681 catcaatggt gaggtgctct atgaagtgga ccttatcaac atggtggaaa ttgtggacgg
    25741 tgtggacctg atggaagcgc accgcaacgc cctcggcctc tgatatatct gaacggcgcg
    25801 ggataccgcg ccagaaccca attgacagga cagcaaaatg agcgataagc agactgaaaa
    25861 gaccattcaa ctggataccc ccatcaagcg cggtaaaaca gaaatcaccg aaattgtgct
    25921 gcgtaaaccg cagtccggtg cgctgcgcgg tacacgcctg caggccatta tggatatgga
    25981 tgtaaacgcg atgatgaccg tgatcccccg catctccagt ccggcactga ctgcacagga
    26041 aattgcagag atggacccgg cagatctcac tgccatgtcg gttgaggttg tcactttttt
    26101 gttgaagaag tcggtgcttg ccggtttacc gacagcctga cggttgacga tctggtggca
    26161 gatatcgcca ccatttttca ctggccgcca tccgttactg acgttatgcc gctgaccgaa
    26221 gtgctggaat ggcggtataa agcgattcag agaagcgggg ccaacgatga gtgataacaa
    26281 cctgcgtctg caggtcattc ttaatgcggt tgacaagctc acccgcccat ttcgatctgc
    26341 gcaggccagt tcaagagaac tggctgctgc tgtcaaaaaa tcccgcgatg caataaagca
    26401 gcttgatcag gccgggagca gtctggacag cttccgaaag ctgcaggcag aaaatcagaa
    26461 attaggcgac aggctgaact atgcccgcca gcgtgcaaat ttgctcagtc aggaactggg
    26521 agcgatgggg ccgccttcgc aacgtcaggt tgttgctctg ggccgtcaac ggctggctgt
    26581 tcagcgcctg gaagaacgcc agaaaaagct gcagcagcag acggcgcttg tgcgtgctga
    26641 actgtaccgg gcgggaattt ctgcgaaaga cgatgcggga gcaactgccc gtttagcccg
    26701 tgaaacatca cgttataacc aggaactttc gaaacaggag gcgcggctga agcgactggg
    26761 ggaagctcag cgcaggatga atgcagcgcg tgccagttat gcccgttcgc tggaggtgcg
    26821 tgatcgtatt gcaggtgccg gagccaccac cacggctgca gggctggcaa tgggcgcacc
    26881 agtgatggcg gcagtaaaaa gctataccag catggaagat gccatgaaag gtgtggcaaa
    26941 gcaggtcaat ggtctgcgtg acgataatgg caaccgcact gcacgttttt atgaaatgca
    27001 ggatgccatc aaggctgcca gcgaacagtt gccgatggaa aacggtgctg tggatttcgc
    27061 tgcactggtt gaaggtggtg cgcgcatgaa cgtcgcaaac cctgacgaca gttgggaaga
    27121 tcagaaacgt gacctgctgg ccttcgccag tacggcagca aaggcggcaa cagcctttga
    27181 gctgccagcg gatgaactgt cagaaagtct ggggaaaatc gcccagctct acaaaatccc
    27241 cacccgcaat attgaacagc tcggtgatgc gctgaactat ctggatgata acgccatgtc
    27301 gaaaggggca gacatcattg atgtgatgca acgtctgggc ggtgtggctg accgtctgga
    27361 ttatcgtaaa gcggcggcgc tgggttccac cttcctgaca ctgggcgctg cgccagaggt
    27421 tgcagccagt gcagcaaacg cgatggtgcg tgaattgtcc attgccacca tgcaaagcaa
    27481 aagtttcttt gaagggatga atctgctgaa actcaatcct gaagtgattg aaaagcagat
    27541 gacgaaggat gcgatgggaa ccatccagcg cgtgctggag aaggtgaacg cgctgccgca
    27601 ggacaagcgc ctgtctgcca tgactatgtt gtttggtaaa gagtttggcg atgacgcggc
    27661 gaaactggca aacaacctgt cggaactgca gcgtcagtta aaactgacag cgggcaatga
    27721 tgcgctcggc tccatgcaga aagaatccga cattaacaag gattcacttt ctgcgcagtg
    27781 gttgctggtc aaaaccggag cgcagaacac cttcagcagc ctgggcgaaa cgctgcgcca
    27841 gccgctgatg gatattctgt acacggtgaa aagcgtcacg ggggcgttgc gtcgctgggt
    27901 ggaagctaac ccggaactga caggcacact gatgaaagca tcggctgttg tggctgcggt
    27961 taccgtcggc ctcggcacct tagcggtggc gctggctgca gtgctggggc cgctggcagt
    28021 gatccgtctg ggattctctg tgctgggtat caaaacgtta tcttccgtta cggcagcagt
    28081 aacccgaacc agcagcgcgt tgtcctggct ggctggcgca ccactggcac tgctgcgacg
    28141 cgggcttgct tcatcgggca acgccgcagg tttacttact gcgccgttat cgtctttgcg
    28201 ccgcacggca tcactgacgg gaaatgtcct taaaactgta gcaggtgcgc cggttgcact
    28261 tttgcggtct ggattatccg gtttacgtgc tgttgctgtg atgtttatga atcctctggc
    28321 ggtactgcgc ggtggactgg ctgccgcagg cgcggtgctt cgtgtgcttg catctggtcc
    28381 gctggcgatg ctgcgcgttg ccctgtatgc catatctggt ctgttaggtg ctctgctcag
    28441 tccgataggt cttgtggtta ctgcactggc gggcgtggcg ctggttgtct ggaaatactg
    28501 gcaacccatc accgcatttc tcggtggcgt ggtggaagga ttcaaagcgg cggcaggtcc
    28561 catcagtgct gcattcgaac cacttaagcc tgtgtttcag tggattggcg acaaagtaca
    28621 ggcgctgtgg ggctggttta ctgatctgct gacgcccgtt aagtcgacct ctgccgaact
    28681 gcagagtgca gcggcaatgg ggcggcgatt cggggaggca ctggcggaag ggctgaatat
    28741 ggtcatgcat ccgctggact ccctgaaatc cggcgtttcc tggttgctgg agaagctcgg
    28801 cattgtcagt aaagaggccg caaaggcaaa actgccggaa agcgtgacgc gtcagcaacc
    28861 tgcgacggtg aatgcagacg gtaaagtgat gatgccatcg ggtggttttc catcatgggg
    28921 atatggcttt gcggggatgt atgacagcgg cgggtatatc ccgcgcgggc agtttggcat
    28981 cgtcggtgaa aacgggccgg aaattgttaa cggcccggca aatgtgacca gccggagaaa
    29041 tacagctgca ctggctgccg ttgttgccgg aatgatgggc gttgctgccg cgccagcaga
    29101 gcttccaccg ttgcaccctt tggcacttcc cacgaaaggt ggagaagcaa ttgtgagtcg
    29161 cgcagccact gtgccgctcg ttcaacggat tgaggcaccg acgcagatca tcattcagac
    29221 gcagccagga caaagtgcgc aggatattgc gcgggaggtg gcacgccagc ttgatgaacg
    29281 tgaacgcagg ctgaaggcaa aagccaggag taactacagc gatcaggggg gatacgacgc
    29341 atgatgatgg tgctgggatt gtacgtgttt atgctgcgca ccgttccgta tcaggaactg
    29401 cagtatcaac gcagctggcg acatgcggca aacagtcggg taaaccgacg tccgtccacg
    29461 cagtttctgg gaccggaaaa cgacatgctg acgctttccg gtgttcttat gccggaaata
    29521 acaggcggca ggctgtcgtt gctggcactg gagcagatgg cagaacaggg gaaagcatgg
    29581 cccctgattg aaggcagcgg cacgatttac ggcatgtatg tgattgaggg actgaatcag
    29641 actaaaacgg agtttttccg cgacggtatg ccgcgccgga ttgagttcac cctgtcgctc
    29701 aaacgagtgg atgaatccct gtccgatatg ttcggtgatc tcagtgcgca actgaataat
    29761 ctgcaggaca cggcaacgtc tgccttaagc gatatcagta aaatggtggg agggctgctg
    29821 tcgtgaattt cagctctgaa ctgcttaaca aaggcaacaa aactcccgca ttcagcatca
    29881 gtattgaggg cagggatatc accactgtgc tggataaccg cctgatgagt ttgacgctga
    29941 cggataaccg gggctttgaa gcggaccagc ttgatctgga gctggatgac gctgacggaa
    30001 aaatcgtgct gccgcgccgt ggtgcggtta ttacgctggc gctgggctgg aaggggcagc
    30061 cgcttttccc gaaaggggca ttcacagtgg acgagattga acacactggc gcaccggacc
    30121 gcctgactat ccgggcgcga agtgctgatt ttcgggaaac gctgaatacc cgccgtgaaa
    30181 aatcgtggca taagaccacc gtcggggaag tggtgaagga aatagccgcg cggcacaagc
    30241 tgaagatggc actgggtaaa gatctgtcgg ataagcccgt ggagcatata gaccagacta
    30301 atgagagtga cggcagtttt ctgatgcggc tggcgcgaca gtacggtgcc atcgcgtcgg
    30361 tgaaaaatgg caatctgtta ttcatccggc aggggcaggg caaaagcgcc actggtaaac
    30421 ctctgccagt gatcactatc acacgcaagg acggcgacag tcaccgattt accctggcag
    30481 atcgcggagc ctacacgggc gtaattgcca gctggttgca tacccgcgaa cctgcgaaga
    30541 aagaaagcac cacggtgaag cgtaagcgca gaactaagaa gcagaagaaa gagccagaag
    30601 cgaagcaggg cgattacctg gtgggtacgg atgaaaacgt gctggtactt aatcgcactt
    30661 atgccaaccg gagcaacgcc gaacgagcgg cgaaaatgca gtgggaacgc ctgcaacgcg
    30721 gcgttgcgtc attttcgcta caactggcag aagggcgggc agatctctac acggaaatgc
    30781 ctgtgaaggt cagtggtttt aaacagccga tagatgatgc ggaatggacc attacgactc
    30841 tgacacatac cgtcagcccg gataacggtt ttacaaccag tctggagctt gaagtgagga
    30901 ttgatgattt cgaaatggaa tgattcttcg caatggagaa cttttaagtt tgcaaaatgg
    30961 aataatgcgg tatcattatt gtgaatttag caaaaatggg gagagctcga aaaatgatga
    31021 tttgcccact gtgtggaagt gccgcccata ctcgcagcag ttttcaggta tcttcattga
    31081 ccaaagagcg ttacaaccag tgccagaaca ttaactgcag ccatactttt gttacccatg
    31141 aaacttttgt tcgttcgatt gcaacgccaa aagagtcaaa tccggttcag ccgcatccaa
    31201 tgaaatcagg acaggtggcg ctctctcttt gacgctgccg ccattttgtc gccatcgtta
    31261 aaaaacagtg tttctaacat catgatttta aacagcttaa atttcaggca acaaaaaacc
    31321 catcaacctt gaaccgaaat ggcggggttg atgggctcca caaaatgggg acatcaaaga
    31381 aaagcagtgg cactaattaa gactgatgcc ctgcggaaaa gttctgcggt tgtgcaaaaa
    31441 aatttcattt tcagggcaac ttcagtttta tcctaatcct ggccatacca tgacgatgat
    31501 tgtccctgcc agcgtcagca ggacgttggc gattgcgtag gtgcccgcgt agcccagcgc
    31561 agggatgtta ctgcgagctg tatcactgat gatctccatt gccggcgcgc aggtgcgtgc
    31621 gcccatcatt gcgccgaaca acagtgcgcg gttcattcgc aatacataag caccgaacaa
    31681 gaaacagata accacgggca caagactgac aattaatccg gcaatcaaca tctgaccgcc
    31741 aatcgcgccc aggccgttat taataccgct accggcgctc agaccaacgc ctgccataaa
    31801 caccatcaag ccgaactctt tcaccatgct taatgcacct tgcggaatgt aaccgaaggt
    31861 cgggtggtta gcacgcataa agcccagcat aattccggcg aataacaacc cggcagcgtt
    31921 ccccatgccg aaactgaatg tgctgaactg gaaggtgatc atcccgatca tcagcccaat
    31981 aacaaagaag gcgcaaaatg ccagcaggtc agtgacctgg ctgtgaatcg agataaagcc
    32041 gatgcgatcg gcgatggttt ttacgcgacg ggcatcaccg ctgacttgta aaacgtcacc
    32101 tttgttaagc acgacgttgt catctatcgg catctcaatc tggctacgaa tgacgcggtt
    32161 aaggaagcaa ccgtgatcgg tcaacttcag ttgtgcgaga cgtttaccta cagcgttatg
    32221 gtttttaacg accacttctt cagtgacgat acgcatgtcg agaaggtcac gatcgaaaac
    32281 ttctttaccg ttacggaagc tgggatcgag tcgggcatgg gcgtcgggat agcctaccaa
    32341 cgctatttca tcgcccattt gtagcacggc atcaccgtct ggatttgcca gaatcccgtt
    32401 acgtcgaata cgttcaatgt agcagccggt ttgtcgataa atacccagtt cacgcagatt
    32461 tttgccgtcg gtccaggcca ccagctccgg gccgacgcga taggcgcgga tcaccggtaa
    32521 ataaacctta cggttggcat cagtgtccag gccacgttcg cgggcgattt gctgggcgct
    32581 ggtctgtaag tcctgatgct gcaatttcgg caagtaacgc gcaccaacaa tcaaactcac
    32641 cagaccgatt aaataggtta aggcataccc gaggctcaga ttatccagtg ccagtgagag
    32701 ctgcctgctt tccatgccgg aatgacgcag tgtatcgcca gcaccgacca gaaccggtgt
    32761 cgacgtcata gagcctgcta acataccggc cgtcaggcca atatcccagc caaacagctt
    32821 acctaaccct aaggcgatca ccagcgcact gccaaccatc accagtgcta acattaggta
    32881 atttttccca tcgcgaaaaa aaatggaaaa aaagttcggt ccggcttcga ccccgacgca
    32941 gaaaataaac agcataaagc caagattaag cgcatcggtg ttaatgctga aatgttgttg
    33001 gcctaataac agcgatacga ctaaaacgcc aatggaatta cccagttgga tcgaaccaag
    33061 tcgtaacttt ccgagacata gcccaagcgc gaggaccaca aataataaca gaatgtaatt
    33121 cccatttaac aattcggcga cgtttatatt cacggaggct aacttcttgt ttactagtaa
    33181 gctgttgaaa gaaatggtaa tttacgataa tgttttttac cagaattcag ggcgcagatt
    33241 cattcagcgc acctaaacga tagtaaagta acaatatatt ttactagtgt aatcacatta
    33301 ggtatcaacg gctatatgaa ttgcgttggc ctatattagc atggaatgcg aagcggcttt
    33361 atcttactga acgccacact ggcgaaaaat gtgttcgata gacgcagtgt caggaggaac
    33421 gagtgaaaca taaacaacgt tgggcggggg caatctgctg ttttgtcctc ttcattgtgg
    33481 tgtgcctttt tctggcgacg cacatgaaag gcgcttttcg ggctgccggg catcctgaaa
    33541 tcggcttgct atttttcatt cttcctggag cagtcgccag cttcttttca cagcgtagag
    33601 aagtcctgaa acctctgttt ggcgcaatgc tggcggcacc ctgttcgatg ctcattatgc
    33661 ggctgttttt ttcaccgacg cgctcattct ggcaagagct ggcatggtta ctaagcgcgg
    33721 tgttctggtg tgcgctgggg gcactgtgtt tcttatttat cagtagtttg tttaaaccac
    33781 agcacagaaa aaatcagtaa agccctcaac gcgagggctt gtcagacgat caggcgtcca
    33841 gattttcttt cacccatgca gcaaaatcgg tatagccgcc gatatgttgc tgatcgacaa
    33901 aaatctgcgg cacggtttct acgggtttac ctgccttttg ttgtagatct tctttagtga
    33961 tcccttccgc acgaatatct acatactgat actgaaaatc atcgcgttca ttgctcaatt
    34021 tctcagccag atcttttgca cgcacacagt aagggcaacc cggacgacca aaaataacgg
    34081 tttgcattat ttctctcctc atagatttat gcctgtaatg atcacgctaa aatgtattcg
    34141 ctgaaagtag gtttaacctg ttgcattaat tgctaaaagc tataactgtt aaacacaata
    34201 cagtgaaaag ttttagactg aaggctcact ttgcagaggg aagcgtatgc gcgcgatcgg
    34261 taaattgcct aaaagcgtgt tgatactgga atttatcgga atgatgttgc tggcggtggc
    34321 gctgctgtcg gtaagcgact ccctgtcgct gcctgagcca ttttctcggc cagaagtgca
    34381 gattctgatg atttttctcg gtgttttgct catgcttccc gctgcggtgg tggttattct
    34441 tcaggtggca aaacgtcttg ccccacagct gatgaaccgt ccaccgcaat attcacgttc
    34501 agaaagagaa aaagataatg acgccaacca ttgaacttat ttgtggccat cgctccattc
    34561 gccatttcac tgatgaaccc atttccgaag cgcagcgtga ggcgattatt aacagcgccc
    34621 gtgcgacgtc cagttccagt tttttgcagt gcagtagcat tattcgcatt accgacaaag
    34681 cgttacgtga agaactggtg acgctgaccg gcgggcaaaa acacgtagcg caagcggcgg
    34741 agttctgggt gttctgtgcc gactttaacc gccatttaca gatctgtccg gatgctcagc
    34801 tcggcctggc ggaacaactg ttgctcggtg tcgttgatac ggcaatgatg gcgcagaatg
    34861 cattaatcgc agcggaatcg ctgggattgg gcggggtata tatcggcggc ctgcgcaata
    34921 atattgaagc ggtgacgaaa ctgcttaaat taccgcagca tgttctgccg ctgtttgggc
    34981 tgtgccttgg ctggccagcg gataatccgg atcttaagcc gcgtttaccg gcctccattt
    35041 tggtgcatga aaacagctat caaccgctgg ataaaggcgc actggctcag tatgacgagc
    35101 aactggcgga atattacctc acccgtggca gcaataatcg ccgggatacc tggagcgatc
    35161 atatccgccg aacaatcatt aaagaaagcc gcccatttat tctggattat ttgcacaaac
    35221 agggttgggc gacgcgctaa aaccgccacg tcgatgtatg atacgcgggc ttttgaccag
    35281 gtctgacaga gaggtgcagg gtgaaaattg ccatattgtc ccgggatgga acgctctatt
    35341 cgtgtaagcg gctgcgtgaa gccgctatac agcgcggtca cctggttgaa attcttgatc
    35401 cgctttcttg ctacatgaac ataaatcctg cggcgtcttc tattcactac aaaggccgca
    35461 agttacccca ttttgacgca gtgatcccgc gtattggcac cgccattacc ttttatggga
    35521 cggcggcact gcgccagttc gagatgctgg ggagctatcc gctcaatgag tcggtcgcca
    35581 ttgcccgggc gcgtgacaaa ttgcgttcca tgcaactgct ggcgcgtcag ggcatcgacc
    35641 tgcctgtcac gggcattgcg cattcgccgg atgataccag cgatttaatc gacatggtcg
    35701 gtggtgcgcc gctggtggtc aagctggttg aaggcacgca gggaattggc gtcgtgttgg
    35761 cggagacgcg tcaggcggcg gaaagcgtga ttgacgcttt ccgcggtctg aacgcgcata
    35821 ttctggtgca ggaatatatc aaagaggcgc aagggtgcga tatccgctgt ctggttgttg
    35881 gtgatgaagt ggtcgctgcg attgaacggc gagcgaaaga gggcgatttt cgttccaatt
    35941 tgcatcgtgg cggcgcggca agcgtcgcca gtatcacacc acaggagcgt gaaatcgcga
    36001 taaaagccgc gcgaacgatg gcgctggacg ttgctggtgt ggatattctg cgtgctaatc
    36061 gcgggccgtt ggtgatggaa gtgaatgcgt cgccagggct ggaaggaata gaaaaaacaa
    36121 ccggtatcga catcgcgggt aaaatgatcc gctggatcga acgccacgct acgacggaat
    36181 attgcctgaa aacgggtggt tagtcgcaat cacattactg atcatggttt tgcctgcgct
    36241 ttttgcgtaa gctgtgccgg tctttttatc ggaagaggtt gtacaaaatt atgacatcgc
    36301 tggtcgttcc tggtctggat acgttgcgtc aatggctcga tgacctgggg atgagttttt
    36361 ttgaatgtga taactgtcag gctctgcatc tgccccatat gcagaatttc gacggtgtct
    36421 ttgatgccaa aatcgatctg atcgataaca cgatcctgtt ttctgccatg gcggaagtcc
    36481 gaccttcagc cgtattgccg ctggcggcgg atttatctgc catcaatgct agttcgctga
    36541 ccgtgaaagc atttcttgat atgcaggatg ataatctgcc aaagctggtg gtttgccagt
    36601 ctttatccgt tatgcagggc gtaacctatg agcagtttgc atggttcgtg cgtcagagcg
    36661 aagagcagat ttcgatggtc attcttgaag ctaatgccca tcaactgctg ttaccgactg
    36721 acgatgaagg gcaaaacaac gttaccgaaa actatttcct ccactgataa ctcctttcga
    36781 gcacgcagtc gctggtgcag tggctgcgcg ctgcaaaatt atctgctgtt tttaaccttt
    36841 tcttaaagat tgtttcactt atcttgtgtc gatttggctt tatcacatag agcaaatacg
    36901 cataaaaatt tgttaaatac cgttttttaa tccgagctat agtctcaaac cctggctaaa
    36961 gttattcttg cgatgctttt atatagcgag cagtgctggc cgggagagag ttctcttttc
    37021 ttacaccgcg ccgataaaaa atatgcacgt ttattgcata tctttcagtg tgacaacttt
    37081 tgttcgtttg ttaacgaact ttcagaagga aagagatatg accgccttaa ataaaaaatg
    37141 gctatcgggt ctggttgcgg gtgctctgat ggccgtctct gtcagcacgc tcgcggctga
    37201 acaaaaaaca ctccacattt ataactggtc tgattatatc gccccggaca cggtggccaa
    37261 ttttgaaaaa gaaaccggta ttaaagtcgt ctacgatgtt ttcgactcta acgaagtact
    37321 ggaaggcaaa ttaatggcgg ggagcaccgg ctttgacctg gtggtgccgt cagcaagctt
    37381 tcttgaacgc cagctgacag cgggagtttt ccagccgctg gacaaaagca aattgccgga
    37441 gtggaagaat ctcgacccgg aactgctgaa gttggtcgcc aaacacgatc ccgacaataa
    37501 atttgctatg ccctatatgt gggcgacgac cgggattggc tataacgtcg ataaagtcaa
    37561 agcggtgctg ggcgaaaacg cgcccgtcga tagctgggac ttgatcctca agcctgaaaa
    37621 tctggaaaaa ctgaaaagct gtggcgtctc tttcctggat gcgcctgaag aagtttttgc
    37681 taccgtgctg aattatctcg gcaaagatcc caacagcact aaagcggatg attacaccgg
    37741 accggcaacc gatctgctgt taaagctgcg cccgaacatt cgttatttcc attcatcgca
    37801 atacattaac gacctggcaa acggcgatat ttgcgtcgct atcggctggg caggcgatgt
    37861 ctggcaggcg tcaaaccgcg cgaaggaagc gaagaatggc gtgaatgtct ctttctcgat
    37921 tccgaaagaa ggggcgatgg cgttctttga tgtcttcgcc atgcctgcgg atgccaaaaa
    37981 caaagacgaa gcctatcagt tcctgaatta cctgctgcgc ccggatgtag tagcgcatat
    38041 ttccgaccat gtgttctatg ccaacgccaa taaagcagcc acgccgctgg tgagtgcgga
    38101 agtccgtgag aacccgggta tttatccgcc tgcggatgtt cgtgcgaagc tgttcactct
    38161 gaaagtgcag gacccgaaaa tcgaccgtgt gcgcacccgc gcgtggacca aagtgaagag
    38221 cggaaaataa tccgcagcct tagatgccgg gcgggcgcac cacacccgcc ggcaattcgc
    38281 accatcatgg tgcgcttgca cacattcaat gccggagagc tgccgtgaat gacgctatcc
    38341 ctcgccccca ggcgaaaacc cgtaaggcgc tgacgccgct attagaaatc cgcaacctga
    38401 ccaaatccta cgatggccaa catgcggtgg atgatgtcag cctgaccatc tacaaaggtg
    38461 aaatcttcgc gctactgggc gcatccggct gtggcaagtc cacgctgctg cgtatgctgg
    38521 caggttttga acaaccttct gccggacaga taatgcttga tggcgtcgat ttgtcacagg
    38581 ttccgcctta cctgcgcccc atcaatatga tgtttcagtc ttacgcgctg tttccgcata
    38641 tgaccgtgga acagaacatc gcttttggcc tgaaacagga caaactaccg aaagcggaaa
    38701 ttgtcagccg ggtcaatgag atgctcggcc tggtgcatat gcaggagttc gccaaacgca
    38761 aaccgcatca gctttccggt ggtcagcgac agcgtgtggc gctggcccga agccttgcca
    38821 agcgcccgaa actattactg ctcgacgagc cgatgggcgc gctggataaa aagctgcgtg
    38881 accggatgca gcttgaagtg gtggatattc tggagcgtgt cggcgtgacc tgtgtgatgg
    38941 tcactcacga tcaggaagag gcgatgacga tggcgggacg tatcgccatt atgaatcgcg
    39001 ggaaatttgt ccagattggc gagccagaag agatctacga acatccaacc acccgctaca
    39061 gtgctgaatt tatcggttcg gttaatgtct ttgaaggtgt actcaaagag cgtcatgagg
    39121 atgggcttgt gctttattcg cctgggctgg tgcatccact gaaagtcgac gcggatgcct
    39181 cggtggtcga taacgtgccg gtacatgtgg cgctgcgccc ggaaaaaatc atgctttgcg
    39241 aagagccgcc cgccaatggt tgtaacttcg cggtggggga ggtgatacac attgcctatc
    39301 tcggcgatct ttcggtgtat cacgttcgtc tgaaaagtgg gcagatgatt agcgcccagc
    39361 tacaaaacgc ccatcgtcat cgtaaagggt taccgacctg gggcgacgaa gtgcgtttgt
    39421 gctgggaagt ggacagctgt gtggtgctga cggtttaagg agcaaagatg agtacacttg
    39481 aacctgctgc ccagtcgaaa ccaccgggcg gatttaagct gtggttgtcg cagctgcaaa
    39541 tgaagcatgg gcgcaaactg gtcattgcgt tgccgtatat ctggttgatc ttgctgtttc
    39601 tgctgccatt tctgattgtc tttaaaataa gcctggcaga gatggcgcgc gctattccac
    39661 cttataccga actgatggag tgggctgacg ggcaactttc catcactctt aatctcggta
    39721 attttctgca actgaccgac gatccgctct attttgatgc ttatctccag tcgttacagg
    39781 tagcggtgat ttcgactatt tgttgtttac tgatcggcta tccgctggcg tgggcggtgg
    39841 cgcacagtaa gccctcgacc cgtaatatct tattactact ggtgatcctg ccgtcgtgga
    39901 cctcgtttct gatccgcgtt tatgcctgga tgggaatatt aaaaaacaac ggtgtgctga
    39961 ataattttct gctgtggctg ggggttatcg atcaaccgct gaccattctg cataccaatc
    40021 tggccgttta tatcggcatt gtttacgctt acgtgccgtt tatggtactg ccgatttata
    40081 ccgcgttgat tcgtattgat tattcgctgg tggaagcagc gctggatctc ggtgcacgac
    40141 cgctgaaaac gttctttact gtgatcgtgc cgctgactaa aggtgggatt attgccggat
    40201 cgatgctggt gtttatcccg gctgtgggcg agtttgtgat cccggaactg ctcggtggcc
    40261 cggacagcat catgatcggg cgcgtgctat ggcaagagtt tttcaataac cgcgactggc
    40321 cggtggcctc ggcggtagcg atcatcatgt tgctgctgct aattgtgccg ataatgtggt
    40381 tccacaaaca ccagcaaaaa agcgtgggag aacacggatg aataatttac cggtagttcg
    40441 ttcgccctgg cggattgtga ttttgctgct gggctttacc tttctctacg cgccaatgct
    40501 gatgctggtt atctattcgt ttaacagctc gaagctggtg acggtgtggg ccggctggtc
    40561 aacgcgctgg tatggtgagt tattgcgcga tgacgcgatg atgagtgcgg ttggtttaag
    40621 cctgacaatt gctgcctgtg cggcaacggc ggcggcgatc ctcgggacta ttgcggcggt
    40681 ggtgctggtg cgctttggca ggtttcgcgg atcaaatggc tttgccttta tgatcaccgc
    40741 gccgctggtg atgccagatg tcatcacggg cttgtcgctg ttgttattat tcgtcgcgct
    40801 tgctcatgcc attggctggc ctgcggaccg cggtatgctc accatctggc tggcgcatgt
    40861 cactttttgt acggcttatg tggcggtcgt tatttcttca agattgcggg aactggatcg
    40921 ctcgatagaa gaagcagcga tggatctcgg tgcgacgccg ctgaaagtgt ttttcgtcat
    40981 tacgctaccg atgatcatgc ccgcgatcat ttctggctgg ttactggctt ttactttgtc
    41041 gcttgatgat ctggtgatcg ccagctttgt ttccgggccg ggagccacca cgttaccgat
    41101 gctggtcttt tccagcgtgc ggatgggggt gaatccggaa atcaacgccc ttgcaacgtt
    41161 aattctcggt gcggtcggaa ttgtcggatt tatcgcctgg tatctgatgg ctcgcgcaga
    41221 aaaacagcgg atacgcgata tccagcgtgc aagacatggc tgaagacact aaaatttgcc
    41281 aacctggcta cataatgccg cgcatgccgc ggcattgttt tcatggaaga cgaaacgttg
    41341 ggatttttta agaaaacatc ttcatctcat gctcgcctga atgtgcctgc gctggtgcag
    41401 gtggcggcgc tcgccattat tatgatccgt ggcctcgacg tgctgatgat tttcaatacg
    41461 ctgggcgtgc gcggtattgg cgagttcatt catcgcagcg tacaaacctg gagtttaacg
    41521 ctggtctttt taagcagtct ggtgctggtt ttcattgaga tctggtgtgc gttttcactg
    41581 gtgaaagggc gtcgctgggc gcgctggcta tatctgctga cacaaatcac cgccgcaagt
    41641 tacttgtggg cggcttcgct ggggtacggt tatccggagc tgttcagcat tcccggtgaa
    41701 tcaaaacgtg aaatcttcca tagcctgatg ctgcagaagc tgccggatat gctcatcctg
    41761 atgctgctgt tcgttccctc gaccagtcgg cggttcttcc agttgcaata atgtgtataa
    41821 tcgtcgcccc tgatgatgtg aaggtcaatg tatgcagtgc gcactttacg acgcgggtcg
    41881 ctgtcgttcc tgtcagtgga taacgcagcc gattccagag caactctccg ctaaaaccgc
    41941 cgatcttaaa aatctgctcg ccgattttcc ggttgaggaa tggtgcgcgc cggtgtcagg
    42001 cccggaacaa gggtttcgta ataaagccaa aatggtggtg agtggtagcg ttgaaaaacc
    42061 actgctcggt atgctgcatc gcgacggcac accagaagac ctttgtgact gcccgcttta
    42121 tcctgcctca tttgcgcccg tttttgcggc gctaaaaccg tttatcgccc gtgcggggct
    42181 gacaccctac aacgtggcgc gtaagcgtgg cgaactgaaa tacattctgc tgactgaaag
    42241 ccagagcgat ggcggcatga tgctgcgctt tgtactgcgt tctgaaacca aactggcaca
    42301 actgcgtaag gcgctgccgt ggttacagga acaactaccg caactgaaag ttattaccgt
    42361 caatattcag ccggtacata tggcgattat ggaaggggag acggagatct accttactga
    42421 acaacaggcg ctggcagagc gttttaatga cgtaccgctg tggatccgtc cgcaaagttt
    42481 cttccagact aatccggcgg tcgccagcca gctgtacgct accgcgcgcg actgggtacg
    42541 gcagctgcca gttaaacata tgtgggatct gttctgcggt gtgggtggct ttggtttaca
    42601 ctgcgcgacg cctgacatgc agttaaccgg gatcgaaatt gcatcagagg ccattgcctg
    42661 tgcaaagcag tcagccgctg aactgggctt aacgcgtttg caatttcagg cgctggactc
    42721 cactcagttt gccaccgctc agggggaggt gccggagctg gtgctggtta atccgccgcg
    42781 ccgcggcatt ggtaaaccgc tatgtgatta tctctcaaca atggcaccgc gttttattat
    42841 ctactccagc tgtaacgccc aaaccatggc gaaagatatc cgcgaactgc caggttaccg
    42901 catcgaacgg gtacagcttt ttgatatgtt cccgcacacc gcgcactatg aagtgctgac
    42961 gctgctggtg aagcaataaa aaagccgcag gtgcggcttc agattgctga tcaggtgcgc
    43021 tttgttcatg ccggatgcgg cgtgtacgcc ttatccggcc tacaaaatca tgctaaatca
    43081 atacattgca gagttgatgt gggccggata agcgcagcgc atcaggcaat cttacttttg
    43141 tcatcagtct caattactgc ggaaaccact ggtcattgat tttctgatat gtaccgtcag
    43201 ctttaattgc tgccagcgcg ttattcagtt tttccagcag agctttgtta tccggacgta
    43261 cagcgatgcc cagaccggtg ccgaagtatt gcggatcggt cactttttca gtagcaacgc
    43321 ccagttgcgg attggttttc agccattcgt ttaccaccgc tgtgtcacca aataccccat
    43381 caatacgacc atttttcaga tcgataaagg cattctgata actgtcatag gagacagttt
    43441 tcacttcggg gtgctgatcc tgaatatatt tctggtgcgt ggtgccgttt tccatcccaa
    43501 tacgtttgcc tttcagatcg gcaaacgttt tgtaggtatc ttttttggca atcacgacgg
    43561 ctgagttttc atagtagggc gtggtaaacg atacctgctt gctacgctcg ggcgtgatat
    43621 ccataccgga gattacggcg tcatattttc tgaatttcag tgacgggatc aggctgtcga
    43681 acgcgtgatt agtaaaagta cattctgcct gcatttgttt gcacaaggct tttgccagat
    43741 cgatatcaaa gccgacaatc tcattattag cacctataga ttcaaagggt ggataggtgg
    43801 ctgaaacgcc aaaattgatt ttctctgcgg cagaagcacc gaaagtaaag gaagcaagta
    43861 aagcggcaag aactaacttt ttcatgatgg aactcccgtc tgtcaatctt atgatttttg
    43921 gccgtgtctg cggcatggga taacaatgcc atcaagtgaa tttatatgca ataaatatga
    43981 ttaaataatt taaatgaaat aaaaaaagac ggatacctta gtgagttgtc cgtcttcatt
    44041 ataagaattt atgcactatg taggccggat aaggcgtcca cgccgcatcc ggcacaggca
    44101 ccgtgctgat gtctgatgcg acgctggcgc gtcttatcag acctacaaac ccccccggcg
    44161 aatgtacgca gctacattaa tttcgccgtt cgaatgccag tgctttgcgc tcgatcagac
    44221 gcatcatcag cgtcagcagg ccgttgacga ccaggtaaat aatccctgcc gcaccgaaca
    44281 ccattacatc gtaggtgcgt ccgtacaaca actggctgta tcccatcact tccatcagcg
    44341 taatggtgta agccagagag gtacttttga acaccagcac cacttcgttg gaataagaag
    44401 agagcgagcg tttaaaggca tacggcagca ggatcgccag cgtatctttt ttgctcattc
    44461 ccagggcgct acaggactgc cactgacctt ctgggatcgc acgaattgca ccgtaaaaca
    44521 gctgcgtggt atacgccgca ctgttcagcg ataacgcaat cagcgcacat aaccacggtt
    44581 ctgacaacaa atgccacagt gccggatact cctgcaaagt cggaaactgg cccggcccgt
    44641 aataaatcag gaagatctgc accagcagcg gcgtaccggt aaacagcgtg atataacccc
    44701 gcaccagcca caccagcacc ggcgttttca gcgtcaggat gatggtaaaa atcaatgcca
    44761 gaatcagtgc cacaatcagc gaggcaacgg ttagcgtcag gctggtgtgt agccctttca
    44821 tcagttcggg taaatactca aacattagct gggcctccgc tcaaaacgtg tcgcgcgcag
    44881 gtcaatgcgt ttgagaatgt actgactgag cagggtgatc accaggtaaa tcgccgccgc
    44941 cacaatgtac caggtaaatg gttcctgggt acgagtagcg atgctttttg tttgcagcat
    45001 taaatcattc acactaatca aactgaccag cgcggtatct ttcagcagca ccagccactg
    45061 gttaccgagg ccaggcagcg catgacgcca catctgcggc atcaccagac ggaaaaagat
    45121 agccgatttc gacagcccca gcgcctgacc agactcccac tgacccaccg gcaccgcttt
    45181 caacgcgccc cgaagcgttt gcgaggcata ggcggcatac agcagtgaca gagcgatgac
    45241 accacaaagg aacgggctaa cgtcgaagtt ctcaatgtcc atctgcactg ggatctgcac
    45301 gaacccaaga ttgatagtga agccatccga aagcgtcagc agcagctgcg aggagccaaa
    45361 atagataaac agcaccacca gaatttctgg caggccacgc agaatggtta ccagcgctga
    45421 acctgcccac gcgacaggac gccattttgc cgactcccat accgcaaaga acatcgccag
    45481 cgccagcccg acaatcaatg cacaaacggc aaggccgacg gtcatcccgg cggcgcttgc
    45541 taaaggaaaa aattcattca tcaggaatta cttctggaac catttgttgt agatggtttc
    45601 gtaagtgcca tctttcttca ctttttccag cgcagtgttg agtttctgct gcagctcagt
    45661 gttgccctga cgtaccgcga tgccgaggcc agtgccgaag taatctttat cggtcacttt
    45721 gtcgcccacc gccgccagtt tcgggttatc tttcagccac tcagtgacca ctgcggtgtc
    45781 accgaagacg ccgtcgatac gcccgttttg cagatccagt tttgcgttct ggtagctgtc
    45841 atacggaacg gtagtgattt ccgggtgctt atccataatg aatttctggt gtgtcgtccc
    45901 gttctgtacg ccgacttttt tgcctttcag ctgatcaaca ctggtgtatt tgccttgctg
    45961 acccacaaac agggcagagt tgtcatagta cggggtggta aacagcacct gcttttcacg
    46021 ctccggagtg atatccatgc ccgccatcac ggcttctacg cgacggaatt tcaggcttgg
    46081 gatcaggctg tcaaacgcct ggttagagaa agtgcaggtt gcatcaatct ctttacacag
    46141 cgcttgtgcc aggtcgacgt caaaaccaac gatctggttg tttgcatcaa tcgattcaaa
    46201 cggaggatag gatgcttcgg tagcaaaacg aatggtttcg gcagctgtgg cggaaagact
    46261 aaaacctgca attaacgcgg caatcagaac ttttttcatt gttgttatcc cgaatcttag
    46321 tgagagagat agtttttaaa tgcttcggtt tgcggctcgg taaagcagct cgcgtcgcct
    46381 tgttctacga tatgaccatt ttccatatac accactcggc tggcggtttt acgcgccact
    46441 tcaacttcgt gggtgacgat cacctgggta atattcgttt ctgccagctc acgaatgatg
    46501 ctgacgattt gtgccgtaat ttccgggtcc agtgcggcgg tcggttcatc gaacagcagt
    46561 acctgcggtt ccatcatcaa cgcacgggca atagcaacac gctgctgctg accaccagaa
    46621 agatgcagcg ggtaacgatc gctataaggt ttgagacgca gacgttccag cagtttttct
    46681 gcacgggcca gcgccagatc tttactcaac cccagtacac ggcagggcgc ttcaatcagg
    46741 ttttgctgca cggtcagatg cggccacagg ttgtattgct gaaacaccat gccaacgtta
    46801 cgacgcaaat cgcgaatcgc tttgtcagag ggtgttttgg taaaatcgaa atggttgcct
    46861 gcaatgttga gcgtaccgga gcgcggcatc tcaagcagat tgagtacacg cagcagcgag
    46921 cttttacccg cgccgctggg gccaagtaac accagcgttt cgccctgtgg gcaatccagc
    46981 gtgatatcga acagcgcctg atgcgcgccg tagaagcaat taatgccgtt taattgaata
    47041 ctcattgaca ctcgtatact ggcagtctga tagctattga ggtcgaagat agtacctttg
    47101 acagaataat tatgcaatat ttctgcttta aaagttaaaa gcaaagcgca ttattcaata
    47161 aacatagcac aaaataacgg gggcggtggt cggtgagcat aaatgtcggc attcctcacg
    47221 aaatgccgga caatttacgg ggtttattgg ttgatcaagg cgttagcgat tctcgatgga
    47281 ctgacggagc gtacccgccg tggcatgaac gctgccgcct aaatagcgta catcgtcgat
    47341 cacccaacac tggccttcct gaatcattaa cacttcatcc tgccagctct gatcaccctg
    47401 ttttagatcc acgcgcaatg gaatgttacg ggcatcacga ttagggatag tcgatgcact
    47461 ggcaacgtgg gcgctgtctg gcaaggtggt tcgactggag aatggatcgt tggtcagtag
    47521 ttcccgatgg ttattatccc gggaggcatc gctaagcagt gtcgccagtt tgtcgctcag
    47581 ataagggcgc aaggcggtga tgtcgttgct gcggtgcaaa atgcggtagt cataaaattg
    47641 ctgggccacg ttatccgggc ctccttcaac gcaaggacca ctgcgtgtgc cgttatcttt
    47701 ataagctgga gtgactgtgg tgcaggcact gaggagcagt gcgcagggga taagcattgt
    47761 caatttgctg tagcgcataa tgatttcctt ataagcgatc gctctgaaag cgttctacga
    47821 taataatgat atcctttcaa taatagcgta tcagtccgat aatgcttttg agatcgaagg
    47881 cttaacaaac aaggagatcg atcatgcaat tttctacaac cccaactctg gaaggccaga
    47941 ccatcgttga atattgcggt gtggtgaccg gcgaagcgat tttaggtgcc aatattttcc
    48001 gtgatttctt tgctggtatc cgcgatatcg ttggcggacg ctccggtgcg tatgaaaaag
    48061 aactgcgcaa agcgcgggag atcgcctttg aggaacttgg ctcccaggcc cgggcgctgg
    48121 ggaccgatgc cgtcgtcggt attgatatcg actacgaaac ggtcgggcaa aacggcagta
    48181 tgctgatggt tagcgtcagc ggtacggcgg tgaaaacgcg tcgatgagaa gagtcttctg
    48241 gctgatcgct gccgctctgt tactggcagg gtgtgcaggc gaaaaaggca ttgtcgagaa
    48301 agagggatat cagcttgata cccgacgcca ggcgcaggcg gcgtatccgc gcattaaagt
    48361 gctggtgatc cactacaccg cagatgattt tgatagctcg ctggcgacac tgaccgataa
    48421 gcaggtcagc tcgcattatc tggtccctgc ggtaccaccg cgatacaacg gtaaaccgcg
    48481 catctggcaa ctggtgccgg aacaagaact ggcctggcat gcggggatta gcgcctggcg
    48541 cggggcaacg cgccttaacg acacctctat tggcattgag ctggaaaacc gtggctggca
    48601 aaaatcggcc ggagtgaaat attttgcccc gtttgaaccg gcacagattc aggcgcttat
    48661 tccactggcg aaagatatta ttgcccgtta tcacatcaag ccggaaaacg tagtggcaca
    48721 tgcggatatc gcaccgcagc gcaaagacga tccggggcca ttatttccct ggcagcaact
    48781 ggcgcagcag gggattggtg cctggccgga tgcgcagcgg gttaactttt accttgccgg
    48841 gcgcgcgccg cacacaccgg tagataccgc gtcattgctg gagcttttgg cgcgctacgg
    48901 ttatgacgtt aaacctgata tgacaccgcg cgagcagcgg cgcgtgatta tggcattcca
    48961 gatgcatttc cgcccgacgt tatataacgg cgaagcggat gcagaaactc aggcgattgc
    49021 cgaagcattg ctggagaaat acgggcagga ttagcgcggc agttttccgt ggtcgcgtag
    49081 ccaggcggca gttttctcga taccttcatc cagggtgatg accggctgat aacctaactc
    49141 ttcctgcgca cgcgtaatat ccagcgtaaa gtcaaaattc aacttggaga cgccgtagtg
    49201 ggtcagcggc ggctcttttg ctgacttgcg gcctaaacgc tccatgctgc gggcgatcat
    49261 atccagcatc gggtagggga cggaacgaat acgacagtca atattcaact cgtcgatcag
    49321 cttctgcacg atgctgcgca gtgtgcgatg ctcgccgttg gtgatgttgt acacacgccc
    49381 ggaaggtagc ttatcgcagg cttcctggct tgccagccac attgcgtgca cggcattttc
    49441 atagtaggtc atatcgacca gcgcactgcc gccatgcggt aacagaatac tgccgtagtg
    49501 gtgcatcata tgcgccagac ggggaataaa gactttatcg tgcggtccga acagactttg
    49561 tgggcgcaga atagtaaagc gcgtttgtgg attcgcctgc gaaagcatat tgatcacttc
    49621 ttcgctggct gctttgctgc gggcaaactc gttggcgaag cggtgagggc gaaaatcttc
    49681 tttaatatcg cgatggtggt gataatcgaa gtacagggag ggggaagaga tatgaataaa
    49741 gttacgcaca ccccaggcga cagcccattc acccaggcgg cgagtggcgc gaacgttagc
    49801 cagatcgaaa gcctgttgtg tcccccaggg tgaggtaaag ctggagcagt gccacagcgt
    49861 atcaatgccc gcgagcatca ctttagcttg tgatgaaacc agctcggtca gatccgccgg
    49921 aacaaactct gcgcccattt tttccagcaa tttgcccatt gcctcgttgc gaccggtcgc
    49981 tcgcacgctg atgcctttct ggcataaaaa ctctatcgcg tttcgaccta agccgctggt
    50041 ggcgccggta accagtacct tcatatcaat ccactgttgt tgagaaaata acgtgcgcat
    50101 tcttccgtga tttcccccat gatgcaatgg gaaacatgaa agaataacgc aggttttgtc
    50161 gattaatctg tgctttgttc tgccagtctg gcgatttgtt ttgccattcc gcgaaaaata
    50221 aacagatgcg cggggatcat caataaccag taaaacagcc ccggcatacc gtgcggatgc
    50281 cagaaagcgc ggacatcgat agtacgatag tcgcctttat cttccaggct aaaacacagt
    50341 cgtcccagcc ccggcgcttt catgccaaat aacaacgtaa gttgtttttc cggttcaacg
    50401 acaatcactt tccagctatc taccgcatcg ccagtctgta aatattcgcg ctccgggcgg
    50461 cctttcgcca gcttatgacc gatcgcgcgg tccatcaacg cccgtgtctg ccacaaaata
    50521 ttgccaaaga aataacgctc tttaccgccg atttggttca ctacctgcca taaagcagca
    50581 aggctggcgg acgttttaac ggtaaacccc gcctgtttgg caaaataacc gtactccggt
    50641 cgccagcggg caaaggcctg agcgtcgtag ccccagtcgc tggagttgac cagtttttcc
    50701 tcctctttca acgtgctacg taccgcgtca tcgaaagcga tcagccgttg tgggatgagt
    50761 gcacgtagcg cggtatcatc cgccagcaga tcgtgtttca gcccctgaat caacgccctg
    50821 gcggtggtgg gcggtacgga agtaatcaca ttgagaaa
//
LOCUS       DAPHDW010000017        49331 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.017, whole genome shotgun sequence.
ACCESSION   DAPHDW010000017 DAPHDW010000000
VERSION     DAPHDW010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49331
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.017"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            26..550
                     /locus_tag="RH057_000963"
     CDS             26..550
                     /locus_tag="RH057_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA"
                     /protein_id="HDV3127375.1"
                     /translation="MKKVLFALSALALTSTSVFAADAGDGTVKFTGEIVDAACVVSPD
                     TQKQEVVLGQVNKSVFTAMGDKSTATPFKIKLENCDISTFKNVEISFNGVGDADNSKL
                     VSVSTEPGAATGVGIGIYDNTNTLVDLNTGKSATVLKEGQTVLYFTANYVSTKDAVTT
                     GYGNAEVDFNLTYN"
     gene            634..1320
                     /gene="lpfB"
                     /locus_tag="RH057_000964"
     CDS             634..1320
                     /gene="lpfB"
                     /locus_tag="RH057_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone LpfB"
                     /protein_id="HDV3127376.1"
                     /translation="MKRISFAVLALLMTAQQCFAGGVALSTTRVIYDGGKKEASLTVE
                     NHNKNEEFLIQSWIDDVNGNKKTPFIITPPLFKLDPDKNNVLRIVNIEDTLPKDRESV
                     YWINVKAIPAKSAENENKNVLQIAVRTRLKLFYRPKGLPGSNLEAMKQLRFQRQNNQI
                     VVNNDSAYNLTFNQFSINGQKIERAGMVLAKGKLNINLPAGTGNAHEVKYSIINDFGV
                     AGEMLTKPIN"
     gene            1344..3875
                     /gene="lpfC"
                     /locus_tag="RH057_000965"
     CDS             1344..3875
                     /gene="lpfC"
                     /locus_tag="RH057_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001759518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane usher protein LpfC"
                     /protein_id="HDV3127377.1"
                     /translation="MHWMHLPLYHYRAHFSFSLLALTIASSLPAYGGKFNPKFLENVQ
                     GIDQHIDLSVYDSPVGQQIPGKYRVSVFVNEEKMASRTLDFSTASEAKRKASGESLMP
                     CLSRVQLEEMGVRVDSFPALKMSPPEACVAFDEIIPQATSRFDFNTQTLHLSFPQAAM
                     MMTARGTVDPSRWDEGIPALLLDYSFSGSNGRNEGTGSSSDSTSDSYYLNLRSGLNVG
                     PWRLRNNSIWNRTDGKNQWDNVGTSLNRAIIPLKSQITLGDTATPGEIFDSVQMRGAL
                     LASDDEMLPDSQRGFAPVVRGIAKSNAEVSIEQNGYVIYRTFVQPGAFEINDLYATSG
                     SGDLTVIIKEADGSEQRFIQPFSAVAIFQREGYLKYSLAAGEYRAGNYDSDKPHFGQF
                     TAMYGLPWGMTAYGGALLSADYSALALGLGKNFGTIGAVSVDVTQAKSQLRNNEKEEG
                     QSYRFLYSKSFEGGTDLRLLGYKYSTSGYYTFQEATDVRSDADSDYRRYHKRSQIQGN
                     ITQQLGDYGSVYFNMTQQDYWNVDGKENSLSAGYHGHIGRVNYSVAYTWTRSPEWEED
                     DRLWSFSVSIPLGGAWSSYRMTTDQNGKTSQQASVSGTLLEDRNLSYNVQQGYTSNGV
                     GYSGSVNMGYMGGSGNIDVGYNYSKDNQQVNYGVRGGVIVHSEGITLSQPLGESLAIV
                     SAPGARGGHVVNSSGVEVDWMGNAVVPYLTPYRETIVELRSDTLGQNVELQEAFQKVV
                     PTRGAVVRSRFDTRVGYRVLMSLKQANGNAVPFGATAALIDESKPASSIVGEEGQLYI
                     SGMPEEGELQVSWGNEQAQRCRVPFRLPENKDNAAIVMVNAVCEK"
     gene            3886..4941
                     /gene="lpfD"
                     /locus_tag="RH057_000966"
     CDS             3886..4941
                     /gene="lpfD"
                     /locus_tag="RH057_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfD"
                     /protein_id="HDV3127378.1"
                     /translation="MKIKNLLAGMVLLGTSTFAGNIWAADWGPCQTSNGVAHEYSFDF
                     VQTIQVPSENKAGKILTQPFALGTKYSAYCECPDPIPDNGVVTYFKGVTLLPEPGTVD
                     GYYKFNNYIDILTKIYVYTQLDIPVPFTDRSNGTAQKECTPYTANNWGTGGKGSISIY
                     ISHPFVGQMIIPKTRVASLFGTKKKGVYNDSQPMANVSISGSITVTQGCELAAGTVLD
                     IPFGEYQAHDFKGRAGQPPQNVQKVQKELSFDCNNISDGVKIYLSIDATPNTAYPSAI
                     DLGNADVGAVIEDGKGNILKPNDSNSLLEMNPGSLYEDVKRKATTTITAYPVSTTGKL
                     PAAGDYSGIATIHVELE"
     gene            4947..5471
                     /gene="lpfE"
                     /locus_tag="RH057_000967"
     CDS             4947..5471
                     /gene="lpfE"
                     /locus_tag="RH057_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbrial protein LpfE"
                     /protein_id="HDV3127379.1"
                     /translation="MKRTILALCLLPLSFSSFAATTDLGPKGTLKFTLKISQGACELV
                     KDSVEVDMGTAVLKKPVRIGTEINPTPFSIGLKNCSEVVRAYVTMDGTPNADDSNLFA
                     LDAGGATGVGLKIKTAAGVQQFPENTTPTPVEFAINGDGAHQLNYIASYVPIRADATI
                     GRADATVDFTVQYE"
     gene            5724..7415
                     /gene="eptB"
                     /locus_tag="RH057_000968"
     CDS             5724..7415
                     /gene="eptB"
                     /locus_tag="RH057_000968"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HDV3127380.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LAVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            7507..7583
                     /locus_tag="RH057_000969"
     tRNA            7507..7583
                     /locus_tag="RH057_000969"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7541..7543,aa:Pro,seq:cgg)
     gene            7618..7751
                     /locus_tag="RH057_000970"
     ncRNA           7618..7751
                     /ncRNA_class="other"
                     /locus_tag="RH057_000970"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            8496..10103
                     /gene="dppA"
                     /locus_tag="RH057_000971"
     CDS             8496..10103
                     /gene="dppA"
                     /locus_tag="RH057_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HDV3127381.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTRELNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            10254..11273
                     /gene="dppB"
                     /locus_tag="RH057_000972"
     CDS             10254..11273
                     /gene="dppB"
                     /locus_tag="RH057_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HDV3127382.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHAQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            11283..12185
                     /gene="dppC"
                     /locus_tag="RH057_000973"
     CDS             11283..12185
                     /gene="dppC"
                     /locus_tag="RH057_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HDV3127383.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVVIVLF
                     IAIFANWIAPYNPAEQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            12196..13179
                     /gene="dppD"
                     /locus_tag="RH057_000974"
     CDS             12196..13179
                     /gene="dppD"
                     /locus_tag="RH057_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HDV3127384.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLVFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            13176..14180
                     /gene="dppF"
                     /locus_tag="RH057_000975"
     CDS             13176..14180
                     /gene="dppF"
                     /locus_tag="RH057_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HDV3127385.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFAPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPALKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(14210..15481)
                     /locus_tag="RH057_000976"
     CDS             complement(14210..15481)
                     /locus_tag="RH057_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV3127386.1"
                     /translation="MQHNTLPKHDQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPATWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLVAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYVTLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRVLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
     gene            15957..16064
                     /locus_tag="RH057_000977"
     CDS             15957..16064
                     /locus_tag="RH057_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3127387.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16440..16547
                     /locus_tag="RH057_000978"
     CDS             16440..16547
                     /locus_tag="RH057_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3127388.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            16923..17030
                     /locus_tag="RH057_000979"
     CDS             16923..17030
                     /locus_tag="RH057_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3127389.1"
                     /translation="MTLAELGMAFWHDLAAPVIAGILASMIVNWLNKRK"
     gene            complement(17117..18796)
                     /gene="bcsG"
                     /locus_tag="RH057_000980"
     CDS             complement(17117..18796)
                     /gene="bcsG"
                     /locus_tag="RH057_000980"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HDV3127390.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDTWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIEFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(18793..18984)
                     /gene="bcsF"
                     /locus_tag="RH057_000981"
     CDS             complement(18793..18984)
                     /gene="bcsF"
                     /locus_tag="RH057_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HDV3127391.1"
                     /translation="MMTISDIIEIIVVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(18981..20552)
                     /gene="bcsE"
                     /locus_tag="RH057_000982"
     CDS             complement(18981..20552)
                     /gene="bcsE"
                     /locus_tag="RH057_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HDV3127392.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSHGPEKIKLFSMLNHEKGLYYLTRDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            20825..21013
                     /locus_tag="RH057_000983"
     CDS             20825..21013
                     /locus_tag="RH057_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HDV3127393.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            21025..21777
                     /gene="bcsQ"
                     /locus_tag="RH057_000984"
     CDS             21025..21777
                     /gene="bcsQ"
                     /locus_tag="RH057_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HDV3127394.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCDHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINNFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            21774..24392
                     /gene="bcsA"
                     /locus_tag="RH057_000985"
     CDS             21774..24392
                     /gene="bcsA"
                     /locus_tag="RH057_000985"
                     /EC_number="2.4.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417990.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
                     /protein_id="HDV3127395.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYPHINASRPRPLDPVRYLIQTCWLLIGTSRKETPKPR
                     RRAFSGLQNIRGRYHQWMNELPERVSHKTQHLDEKKELGHLSAGARRLILGIIVTFSL
                     ILALICVTQPFNPLAQFIFLMLLWGVALIVRRMPGRFSALMLIVLSLTVSCRYIWWRY
                     TSTLNWDDPVSLVCGLILLFAETYAWIVLVLGYFQVVWPLNRQPVPLPKDMSLWPSVD
                     IFVPTYNEDLNVVKNTIYASLGIDWPKDKLNIWILDDGGREEFRQFAQNVGVKYIART
                     THEHAKAGNINNALKYAKGEFVSIFDCDHVPTRSFLQMTMGWFLKEKQLAMMQTPHHF
                     FSPDPFERNLGRFRKTPNEGTLFYGLVQDGNDMWDATFFCGSCAVIRRKPLDEIGGIA
                     VETVTEDAHTSLRLHRRGYTSAYMRIPQAAGLATESLSAHIGQRIRWARGMVQIFRLD
                     NPLTGKGLKFAQRLCYVNAMFHFLSGIPRLIFLTAPLAFLLLHAYIIYAPALMIALFV
                     LPHMIHASLTNSKIQGKYRHSFWSEIYETVLAWYIAPPTLVALINPHKGKFNVTAKGG
                     LVEEEYVDWVISRPYIFLVLLNLVGVAVGIWRYFYGPPTEMLTVVVSMVWVFYNLIVL
                     GGAVAVSVESKQVRRSHRVEMTMPAAIAREDGHLFSCTVQDFSDGGLGIKINGQAQIL
                     EGQKVNLLLKRGQQEYVFPTQVARVMGNEVGLKLMPLTTQQHIDFVQCTFARADTWAL
                     WQDSYPEDKPLESLLDILKLGFRGYRHLAEFAPSSVKGIFRVLTSLVSWVVSFIPRRP
                     ERSETAQPSDQALAQQ"
     gene            24403..26742
                     /gene="bcsB"
                     /locus_tag="RH057_000986"
     CDS             24403..26742
                     /gene="bcsB"
                     /locus_tag="RH057_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HDV3127396.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMPGVQGADAPVVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNLEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLAAISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            26749..27855
                     /gene="bcsZ"
                     /locus_tag="RH057_000987"
     CDS             26749..27855
                     /gene="bcsZ"
                     /locus_tag="RH057_000987"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HDV3127397.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRVAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYLPPTLAQYFTRFGAPWTTLRETNQRLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDAYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            27837..31310
                     /gene="bcsC"
                     /locus_tag="RH057_000988"
     CDS             27837..31310
                     /gene="bcsC"
                     /locus_tag="RH057_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsC"
                     /protein_id="HDV3127398.1"
                     /translation="MRKFTLNIFTLSLGLAVMPMVEAAPTAQQQLLEQVRLGEATHRE
                     DLVQQSLYRLELIDPNNPDVVAARFRSLLRQGDIDGAQKQLDRLSQLAPSSNAYKSSR
                     TTMLLSTPDGRQALQQARLQATTGHAEEAVASYNKLFNGAPPEGDIAVEYWSTVAKIP
                     ARRGEAINQLKRINADAPGNTGLQNNLALLLFSSDRRDEGFAVLEQMAKSNAGREGAS
                     KIWYGQIKDMPVSDASVSALKKYLSIFSDGDSVAAAQSQLAEQQKQLADPAFRARAQG
                     LAAVDSGMAGKAIPELQQAVRANPKDSEALGALGQAYSQKGDRANAVANLEKALALDP
                     HSSNNDKWNSLLKVNRYWLAIQQGDAALKANNPDRAERLFQQARNVDNTDSYAVLGLG
                     DVAMARKDYPAAERYYQQTLRMDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRR
                     SIDDIERSLQNDRLAQQAEALENQGKWAQAAALQRQRLALDPGSVWITYRLSQDLWQA
                     GQRSQADTLMRNLAQQKPNDPEQVYAYGLYLSGHDQDRAALAHINSLPRAQWNSNIQE
                     LVNRLQSDQVLETANRLRESGKEAEAEAMLRQQPPSTRIDLTLADWAQQRRDYTAARA
                     AYQNVLTREPTNADAILGLTEVDIAAGDTAAARSQLAKLPATDNASLNTQRRVALAQA
                     QLGDTAAAQQTFNKLIPQAKSQPPSMESAMVLRDGAKFEAQAGDPKQALETYKDAMVA
                     SGVTTTRPQDNDTFTRLTRNDEKDDWLKRGVRSDAADLYRQQDLNVTLEHDYWGSSGT
                     GGYSDLKAHTTMLQVDAPYSDGRMFFRSDFVNMNVGSFSTNADGKWDDNWGTCTLQDC
                     SGNRSQSDSGASVAVGWRNDVWSWDIGTTPMGFNVVDVVGGISYSDDIGPLGYTVNAH
                     RRPISSSLLAFGGQKDSPSNTGKKWGGVRADGVGLSLSYDKGEANGVWASLSVDQLTG
                     KNVEDNWRVRWMTGYYYKVINQNNRRVTIGLNNMIWHYDKDLSGYSLGQGGYYSPQEY
                     LSFAIPVMWRERTENWSWELGASGSWSHSRTKTMPRYPLMNLIPTDWQEEAARQSNDG
                     GSSQGFGYTARALLERRVTSNWFVGTAIDIQQAKDYAPSHFLLYVRYSAAGWQGDMDL
                     PPQPLIPYADW"
     gene            31392..33380
                     /gene="hmsP"
                     /locus_tag="RH057_000989"
     CDS             31392..33380
                     /gene="hmsP"
                     /locus_tag="RH057_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HDV3127399.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
     gene            33563..34849
                     /gene="dctA"
                     /locus_tag="RH057_000990"
     CDS             33563..34849
                     /gene="dctA"
                     /locus_tag="RH057_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HDV3127400.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGAFGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
     gene            35070..36566
                     /locus_tag="RH057_000991"
     CDS             35070..36566
                     /locus_tag="RH057_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV3127401.1"
                     /translation="MQGTKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLTESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASNSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAAATSVATDDVHPEVTDIPPAQ"
     gene            complement(36662..37591)
                     /gene="kdgK"
                     /locus_tag="RH057_000992"
     CDS             complement(36662..37591)
                     /gene="kdgK"
                     /locus_tag="RH057_000992"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HDV3127402.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLESEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRANG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEGLVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            37823..38590
                     /gene="pdeH"
                     /locus_tag="RH057_000993"
     CDS             37823..38590
                     /gene="pdeH"
                     /locus_tag="RH057_000993"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HDV3127403.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVAHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFIEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPIETLNTAVLAL"
     gene            38660..40720
                     /locus_tag="RH057_000994"
     CDS             38660..40720
                     /locus_tag="RH057_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV3127404.1"
                     /translation="MSKAGKITAAISVAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGDLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYVFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKVDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(40954..42276)
                     /locus_tag="RH057_000995"
     CDS             complement(40954..42276)
                     /locus_tag="RH057_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV3127405.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVASI
                     LGASVAPYIAAWLQTNYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(42687..43700)
                     /gene="yhjD"
                     /locus_tag="RH057_000996"
     CDS             complement(42687..43700)
                     /gene="yhjD"
                     /locus_tag="RH057_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HDV3127406.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            complement(43749..44648)
                     /locus_tag="RH057_000997"
     CDS             complement(43749..44648)
                     /locus_tag="RH057_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3127407.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPPGI
                     AKSLLLPRLSEFLYLHPGIELELSSHDRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            45168..45770
                     /locus_tag="RH057_000998"
     CDS             45168..45770
                     /locus_tag="RH057_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV3127408.1"
                     /translation="MQIVMFDRQSIFIHGMKISLQQRIPGVSIQGASQADELWQKLES
                     YPEALVMLDGDQDGEFCYWLLQKTVVQFPEVKVLITATDCNKRWLQEVIHFNVLAIVP
                     RDSTVETFALAVNSAAMGMMFLPGDWRMTPEKDIKDLKSLSARQREILTMLAAGESNK
                     EIGRALNISTGTVKAHLESLYRRLEVKNRTQAAMMLNISS"
     gene            complement(45821..47470)
                     /locus_tag="RH057_000999"
     CDS             complement(45821..47470)
                     /locus_tag="RH057_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV3127409.1"
                     /translation="MLNQKIQNPNPDELMIEVDLCYELDPYELKLDEMIEAEPEPEMI
                     EGLPASDALTPADRYLELFEHVQSAKIFPDSKTFPDCAPKMDPLDILIRYRKVRRHRD
                     FDLRKFVENHFWLPEVYSREYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQ
                     SYIVPGGRFSETYYWDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYY
                     LSRSQPPVFALMVELFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMP
                     DGSLLNRYWDDRDTPRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRD
                     TGRLASIRTTQFIPIDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRY
                     LWDDENGIYRDYDWRREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGIL
                     ASEYETGEQWDKPNGWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMI
                     EKYHIADGVPREGGGGEYPLQDGFGWTNGVVRRLIGLYGEP"
     gene            47875..49272
                     /gene="ccp"
                     /locus_tag="RH057_001000"
     CDS             47875..49272
                     /gene="ccp"
                     /locus_tag="RH057_001000"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HDV3127410.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHALNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
BASE COUNT        11968 a        12267 c        13389 g        11707 t
ORIGIN      
        1 tttatctata taaatggagt tataaatgaa aaaggttctg tttgccttat ctgcgcttgc
       61 gctgacctct acttccgtat tcgcagctga tgcgggcgac ggtactgtta aattcaccgg
      121 tgagattgtt gacgccgctt gtgttgtgtc tccagacact caaaaacaag aagttgttct
      181 gggtcaggtg aacaagtctg tatttactgc tatgggtgat aaatccacag caacgccgtt
      241 caaaatcaaa ctggaaaact gcgatatctc cactttcaaa aatgtggaaa tcagctttaa
      301 cggcgttggc gatgcagaca acagcaaact ggtttctgta agcactgaac caggtgccgc
      361 aactggcgta ggtatcggta tttatgataa caccaatacc ctggttgacc tgaatactgg
      421 taaatctgct actgtcctga aagaaggtca gaccgttctg tatttcaccg ctaactatgt
      481 ctctaccaaa gatgcagtaa ccacgggtta cggtaacgct gaagtcgact tcaacctgac
      541 ttacaactaa tcgatcctga cggaacgatc atctctcaat ggcaacagag actgtgttgc
      601 cattattcac accaatgact cagggaccga atcatgaaac gtattagctt tgctgtgctg
      661 gcacttttaa tgaccgcaca acagtgtttt gctggtggcg tagccttaag cacgacgcgt
      721 gttatttatg atggcggcaa gaaagaagcg tcgctgaccg ttgaaaacca taataaaaat
      781 gaagagtttt tgatccagtc atggattgat gatgtgaatg gcaataaaaa aacaccgttt
      841 attattacac cgccattatt caaacttgat cctgataaaa ataacgtctt acgtatcgtc
      901 aatattgaag ataccttacc taaagatcgt gaatccgttt actggattaa tgtaaaggcc
      961 attcctgcga aaagtgctga aaatgaaaat aaaaacgtct tacagatagc cgttcgcacc
     1021 cgtttgaagt tgttctatcg tccaaaaggt ttgccgggaa gcaacctcga ggccatgaaa
     1081 cagttacgtt tccagcgtca aaataaccaa attgtggtta ataacgactc cgcttataac
     1141 ctgaccttta atcagttttc cattaatgga caaaagattg aacgggcggg aatggttctc
     1201 gcgaaaggaa agttaaacat taaccttcca gccggtacag gcaacgcgca cgaagtgaaa
     1261 tacagcatta ttaatgattt cggtgttgct ggcgaaatgc tgacgaagcc gattaactga
     1321 tccttttaca aggcgctttt actatgcatt ggatgcattt acctctctat cactacagag
     1381 cgcatttttc cttctctctg ctggcattaa cgattgccag ctcgttacct gcgtatggcg
     1441 gaaaatttaa ccctaaattt ctcgaaaacg ttcagggtat cgatcaacac atcgacttgt
     1501 cggtatacga ctcacctgtc ggccagcaaa tccccggtaa ataccgggtt tcggtgtttg
     1561 taaacgaaga aaaaatggct tcccggacgc tggattttag taccgccagc gaagcgaaac
     1621 gcaaagccag cggtgagtcg cttatgcctt gccttagccg ggtgcaactg gaagaaatgg
     1681 gcgtgcgcgt tgacagcttt ccggcgctga agatgtcacc accagaagcc tgcgtagcgt
     1741 ttgatgaaat cattccccag gccaccagcc gctttgattt taatactcag acgctgcatc
     1801 tttcctttcc acaggcggca atgatgatga ccgctcgcgg cacggttgat ccttctcgct
     1861 gggacgaagg aatacctgcg ctgttgctgg attacagctt tagtggcagc aatggacgca
     1921 atgaaggtac aggttcatct tcagattcga ccagtgacag ttattacctg aacctgcgca
     1981 gcggtttgaa cgttggtccg tggcgtttac gtaataacag tatctggaac cgtaccgatg
     2041 gcaaaaacca atgggataac gttggtacat cgttaaaccg ggcaattatt ccacttaaat
     2101 cacagataac tctgggtgat accgccacgc ctggcgagat ttttgatagc gtacaaatgc
     2161 gtggggcgtt actggcatcc gacgatgaaa tgttgccgga cagccagcgt ggctttgctc
     2221 cggtagtgcg cggcatcgcc aaaagcaacg ctgaagtgag tatcgaacag aacggctatg
     2281 tcatttatcg cacttttgtt cagccgggtg catttgagat taacgatctc tacgcaacct
     2341 ccggcagcgg cgacctgacg gtgatcatta aagaagccga cggaagtgaa caacgtttta
     2401 tccagccgtt ttcggcggtg gcgattttcc agcgtgaagg gtatctgaaa tacagtctgg
     2461 ctgccggtga atatcgtgct ggtaattacg acagcgataa accacatttc gggcagttta
     2521 ccgccatgta cggcttgccg tggggtatga ccgcctacgg tggtgcattg ttgtcggcag
     2581 attacagcgc tttagctctg ggcctgggga aaaacttcgg taccattggc gcagtatcgg
     2641 tggatgtcac tcaggcgaaa agtcagttac gcaacaatga aaaagaggaa ggccagtctt
     2701 atcgcttcct ctattcgaaa agttttgagg gtggcactga cctgcgtctg ttgggctaca
     2761 aatactcaac cagcggctac tacaccttcc aggaagcaac tgatgttcgc agtgacgccg
     2821 acagtgatta ccgccgttat cacaagcgca gccagataca gggcaatatt acccagcaac
     2881 tgggagacta cggctcggtt tatttcaata tgacgcagca ggattactgg aacgttgacg
     2941 gtaaggaaaa ctccttaagc gccggttatc acggtcacat tggtcgggtg aattacagcg
     3001 tggcctatac ctggacgaga agtccggaat gggaagaaga cgatcgcttg tggtcattca
     3061 gtgtttccat ccctctgggg ggcgcgtgga gcagttatcg catgactacc gaccagaatg
     3121 gcaagacgtc gcaacaggcc tctgttagcg gcacgttact ggaagatcgt aacctgagct
     3181 ataacgttca gcagggttac accagtaatg gcgtaggcta cagtggcagc gtcaacatgg
     3241 ggtatatggg tggctctggt aacattgacg taggctataa ctattctaaa gataaccaac
     3301 aggttaacta tggcgtgcgc ggcggtgtca tcgttcacag tgaaggtata acgctttcgc
     3361 agcctttagg cgaaagtctg gcgattgttt ctgcaccggg cgcgcgtggt ggacatgtgg
     3421 ttaacagcag cggcgtagaa gttgactgga tgggcaacgc cgtggtgcct tatctcacac
     3481 catatcgtga aaccattgtg gaattgcgta gcgacacgtt ggggcaaaac gttgaactac
     3541 aggaagcgtt ccagaaagtc gtgcctactc gtggtgcagt tgtgcgttcc cgctttgata
     3601 cccgcgtcgg ttaccgtgtg ctgatgagct taaaacaggc gaatggcaac gcagtaccgt
     3661 ttggtgcaac cgcagcgtta attgatgaga gcaaacctgc cagcagcatt gtgggtgaag
     3721 aaggacagct ttatatcagc gggatgccag aagagggcga attgcaggtg agctggggga
     3781 acgagcaggc gcaacgttgc cgtgtgccat tccgcttgcc agagaacaag gacaatgcgg
     3841 ctatcgtgat ggttaatgcg gtatgcgaga aataaggata tttagatgaa aattaaaaat
     3901 ttgttggcag gtatggtgct gttagggacg agcacttttg ctggaaatat atgggctgcg
     3961 gattggggac catgtcagac atcaaatggg gtggcccatg agtatagttt tgatttcgta
     4021 cagactatcc aggtaccttc agaaaataag gctgggaaaa tattaactca gccctttgct
     4081 ttaggtacaa aatattccgc ttattgtgaa tgcccggatc ccattccaga taatggagtt
     4141 gtgacttatt ttaaaggtgt aacgcttctg cctgagcctg gaactgtaga tgggtactat
     4201 aaattcaata attacattga tattctcacc aaaatatatg tctacacaca attagatatc
     4261 ccggtgcctt ttaccgatcg aagcaatggc acggcacaga aagaatgtac gccctatact
     4321 gctaacaatt gggggacagg tggtaaagga agtatcagta tttatatttc acatcctttt
     4381 gttgggcaaa tgattattcc taaaacacga gttgcgtccc tctttgggac aaaaaagaaa
     4441 ggtgtttaca atgatagtca gcccatggcc aatgtcagta tttccgggag tattacagtt
     4501 actcaaggct gtgaacttgc cgctggcacc gtactggata ttccattcgg agaatatcag
     4561 gcccatgatt ttaaagggcg tgccggacaa ccaccacaaa atgttcagaa ggtacaaaaa
     4621 gaattaagct ttgactgcaa taatatttca gatggcgtca aaatttatct cagtattgat
     4681 gcaacaccaa atactgccta tcccagcgca attgatttag gcaacgccga tgttggtgca
     4741 gtcattgagg acggtaaagg taatattctt aagccgaatg acagtaatag cttattagaa
     4801 atgaacccag gctcgctgta tgaagatgtc aaacgcaaag cgacaaccac aattaccgct
     4861 tatcccgtta gcactaccgg aaaattaccc gctgccgggg attatagcgg tattgctacc
     4921 atacatgtcg aactggagta acagtcatga agcgaacaat acttgcgtta tgcctgctgc
     4981 ctctttcctt ttcgtcattc gcggcaacca cggatttagg gccgaaagga acattgaaat
     5041 tcacccttaa aatcagccag ggtgcttgcg aactggtgaa agacagcgtt gaggttgaca
     5101 tgggcaccgc ggttctcaaa aagccggtaa gaatcggaac ggaaattaat cccactcctt
     5161 tttccatcgg cctgaaaaac tgtagcgaag tggtcagagc ctacgtgact atggacggta
     5221 cacccaatgc agacgactca aatctttttg cgctggatgc gggtggcgct acaggcgttg
     5281 ggctgaaaat caaaaccgct gctggagtac aacagttccc ggaaaataca acgccgacac
     5341 cggttgagtt tgcaattaac ggtgatggcg cacatcaact gaactatatc gccagctacg
     5401 tgcctattcg cgccgatgca actattggtc gcgcagatgc aacggtcgac tttactgtac
     5461 agtacgaata atttcacatc ttaagggcca gttaactggc cctttttact gctttttgta
     5521 acgctaaagt ctcttttcaa acttgcattt ttgtaaattt gtgcttcatg cacactcttt
     5581 ccccacactt tttcgctttg ctgtggtcta cttattcgcg cgtgtagatt ttacttatct
     5641 gactacctcc gcacttttcc cctgccgggc ctgaaaagcc gctaagcagg gtgttatcac
     5701 ctgtttgtcc agggtttgtt tgcatgagat acatcaaatc gattacacag cagaagctga
     5761 gctttttgct tgcaatctat attggccttt ttatgaatgg cgcggttttt tatcgccgct
     5821 tcggcagcta tgcgcacgat tttaccgtct ggaaaggcgt ttctgctgtt gttgaactgg
     5881 ccgccaccgt actggtgacc ttctttttac tacgtcttct atcgctgttt ggccgccgca
     5941 gctggcgtat tctggcgtcg ctggtggtgc tcttttccgc aggtgccagc tattacatga
     6001 ccttccttaa tgtggtcatt ggttatggca tcatcgcttc cgtcatgacc accgatatcg
     6061 acctgtcaaa agaagttgtt ggtctgaact ttattctctg gttaatcgcc gttagtgcat
     6121 tgcctcttat ccttatctgg aataaccgct gtcgctacac cttgctccga caactgcgaa
     6181 ccccagggca gcgtattcgc agcctggcgg tcgtcgtact ggcgggtatt atggtttggg
     6241 caccgattcg tttgctggat atccagcaga agaaagtgga gagggcgacc ggcgttgatt
     6301 tgccgagtta tggcggtgtc gtagcgaact cttatctgcc atcaaactgg ctttctgcgt
     6361 tggggctgta tgcctgggcg cgggtcgatg aatcttccga taataattca ttgcttaatc
     6421 cggcgaagaa attcacttat caggcaccgc aaaacgttga tgacacttat gtcgtgttta
     6481 tcatcggtga aaccacgcgt tgggaccata tgggcatttt cggctatgag cgtaatacca
     6541 cgccgaaact ggcccaggag aaaaatctgg cggcgttccg tggttactcc tgtgataccg
     6601 caaccaaact ctcactgcgt tgcatgtttg tacgtcaggg gggcgcggaa gataatccgc
     6661 agcgcacatt aaaagaacag aacattttcg cggttctgaa gcagttagga ttcagttctg
     6721 acctctacgc tatgcagagc gaaatgtggt tctacagcaa cacgatggcg gacaacattg
     6781 cttatcgtga gcagattggt gcggagccac gtaatcgtgg caagccggta gatgatatgt
     6841 tgctggtaga cgaaatgcag caatcgctag ggcgcaaccc ggatggtaag catctgatca
     6901 ttctgcatac caaaggttcg cattttaact acacccagcg ttatccgcgt agcttcgcgc
     6961 agtggaagcc ggaatgtatt ggtgttgata gcggctgtac caaagcgcag atgatcaact
     7021 cctatgacaa ctcggtgacc tatgtggatc actttatctc cagcgtgatt gatcaggttc
     7081 gcgataagaa agcgattgtg ttctacgcag ctgaccacgg tgagtcaatt aatgaacgcg
     7141 agcacctgca cggcacgccg cgtgaactgg caccgccgga gcagttccgc gtaccgatga
     7201 tggtctggat gtcagataaa tatctggaaa atccggccaa tgctcaggca tttgcgcagc
     7261 tgaaaaaaga agccgacatg aaagtgccac gccgtcatgt agagctgtac gacaccatca
     7321 tgggttgtct tggctatact tcaccggatg gtggaattaa cgaaaacaac aactggtgtc
     7381 acatcccgca gacaaaagtg gcagcggcta actaagcggc ctgctgactt tctcgccgat
     7441 caaaaggcat tttgctatta agggattgac gagggcgtat ctgcgcagta agatgcgccc
     7501 cgcattcggt gattggcgca gcctggtagc gcacttcgtt cgggacgaag gggtcggagg
     7561 ttcgaatcct ctatcaccga ccaaattcga aaagcctgct cagcgagcag gcttttttgc
     7621 atttgaagcc catgaggatg agaacctccg ggggcgggga ggttcgactc gagcgaagcg
     7681 agagaacgtt gcgccagcaa cggcccgcag ggcgagccac gaagtggcga gtaatcctct
     7741 atcaccgacc aaattcgaaa agcctgctca acgagcaggc ttttttgcat ttgtagctca
     7801 tgaggatgag aacctccggg ggcggggagg ttcgactcga cgtaggcctg ataagacgcg
     7861 tcagcgtcgc atcaggcaac ggcaccgcac ttattgtcgg atgcggcgtg aacgccttat
     7921 tcgacctaca cgccacacat ttccctgcca aatttgcgcc ccatatctca ctatccatta
     7981 acggatttgt gacagaaacc attgtctttt tttatatttg cttaaatcta ttttttcgta
     8041 acttatggct ggcgctggca ttttccgctg tgcgttttag cgttcatggt attaatcgct
     8101 cagatatcca ccttttcctc agattttttt cactaaatgc ataaaactta atcaccaaat
     8161 gttgctaaat attaagctga ttgttctggt catgttggag tagcacaaat ttctctgctg
     8221 caaatagctg tttgaagttt ttttaatctt tgtttgtgga ttctcaccgt tggtgtaaat
     8281 cccggttggc tgtattgacg ttttcacatt ctgttgacag attgtaggtc acgaggggca
     8341 ttttatggag gatccgcact gttacactga tgttaattag tacggcatcc ccacctcata
     8401 acgttgaccc gaccgggcaa aaaacaaaaa aggtcaggca gcgacaaccc actgcaaagg
     8461 gttaaaacaa caaacatcac aattggagca gaataatgcg tatttccttg aaaaagtcag
     8521 ggatgctgaa gcttggtctc agcctggtgg ctatgaccgt cgcagcaagt gttcaggcta
     8581 aaactctggt ttattgctca gaaggatctc cggaagggtt taacccgcag ctgtttacct
     8641 ccggtaccac ctatgacgcc tcttccgtac cgctttataa ccgcctggtt gaatttaaaa
     8701 tcggcaccac cgaagtgatt ccgggcctcg ctgaaaagtg ggaagtcagc gaagacggta
     8761 aaacctatac cttccatctg cgtaaaggtg tgaagtggca cgacaataaa gaattcaaac
     8821 cgacgcgtga actgaacgcc gatgacgtgg tgttctcgtt cgatcgtcag aaaaacgcgc
     8881 aaaacccgta ccataaagtt tctggcggca gctacgaata cttcgaaggc atgggcttgc
     8941 cggagctgat cagcgaagtg aaaaaggtgg acgacaacac agttcagttt gtgctgactc
     9001 gcccggaagc gccgttcctc gctgacctgg caatggactt cgcctctatt ctgtcaaaag
     9061 aatatgctga tgcgatgatg aaagccggta caccggaaaa actggacctc aacccaatcg
     9121 gcaccggtcc gttccagtta cagcagtatc aaaaagattc ccgtatccgc tacaaagcgt
     9181 ttgatggcta ctggggcacc aaaccgcaga tcgatacgct ggtcttctct attacccctg
     9241 acgcttccgt gcgttacgcg aaattgcaga agaatgaatg ccaggtgatg ccgtacccga
     9301 acccggcaga tatcgctcgc atgaagcagg ataaatccat caatctgatg gaaatgccgg
     9361 ggctgaacgt cggctatctt tcatataacg tgcagaaaaa accactcgat gacgtaaaag
     9421 ttcgccaggc tctgacctac gcggtgaaca aagacgcgat catcaaagcg gtttatcagg
     9481 gcgcgggtgt atcagcgaaa aacctgatcc cgccaaccat gtggggctat aacgacgacg
     9541 ttcaggacta cacctacgat cctgaaaaag cgaaagcctt gctgaaagaa gcgggtctgg
     9601 aaaaaggttt ctccatcgac ctgtgggcga tgccggtaca acgtccgtat aacccgaacg
     9661 ctcgccgcat ggcggagatg attcaggcag actgggcgaa agtcggcgtg caggccaaaa
     9721 ttgtcaccta cgaatggggt gagtacctca agcgtgcgaa agatggcgag caccagacgg
     9781 taatgatggg ctggactggc gataacgggg atccggataa cttcttcgcc accctgttca
     9841 gctgcgccgc ctctgaacaa ggctccaact actcaaaatg gtgctacaaa ccgtttgaag
     9901 atctgattca accggcgcgt gctaccgacg accataacaa acgcgttgaa ctgtacaaac
     9961 aagcacaggt ggtgatgcac gatcaggctc cggcactgat catcgctcac tccaccgtgt
    10021 ttgaaccggt acgtaaagaa gttaaaggct atgtggttga tccattaggc aaacatcact
    10081 tcgaaaacgt ctctatcgaa taattaaaag ccatacccgg tggcgctgtg cctctgcgtg
    10141 cagcgccatc cggcagcaat acttcattct ctgtccgaca agggcgggaa ttgatttgtg
    10201 agcaatacag acacgcagtt ccaggctgcg ggtcattata gagaatccgg gttatgttgc
    10261 agtttattct ccgacgtttg ggactcgtca tccccacgtt tatcggtatt acccttctca
    10321 catttgcctt tgtccacatg atcccgggcg atccggtgat gatcatggcg ggcgaacgtg
    10381 ggatctcccc tgagcgtcac gcgcagctgc tggctgaact cggcttagat aaaccgatgt
    10441 ggcagcagta tctccattac atttggggcg taatgcacgg cgatctaggc atttcaatga
    10501 aaagccgaat tccagtatgg gaagagttcg tgccgcgctt tcaggccacg ctggaacttg
    10561 gcgtctgcgc gatgattttt gctacggcag tcggtattcc ggttggtgtg ctggctgcgg
    10621 ttaaacgcgg ttccattttc gatcacactg cagttggcct ggcgctgacc ggctactcga
    10681 tgccgatctt ctggtggggc atgatgctga tcatgctggt ttcggtgcac tggaacctga
    10741 cgcccgtctc cggtcgcgtg agcgatatgg tgttcctcga tgactccaat ccgttaaccg
    10801 gttttatgct aatcgacacc gccatctggg gtgaagacgg caactttatc gatgccgttg
    10861 cccatatgat cttaccggcc attgtgctgg gtactattcc gctggcggtc attgtacgta
    10921 tgacacgctc ctcgatgctg gaagtgctgg gtgaagatta catccgcacc gcgcgcgcca
    10981 aagggttgac ccgcatgcgg gtgattatcg tccatgcgct gcgtaacgcg atgctgccgg
    11041 tagtgaccgt tatcggcctg caggtgggaa cattgctggc gggggcgatt ctgaccgaaa
    11101 ccatcttctc gtggcccggt ctggggcgct ggttgattga cgcgctgcaa cgccgcgact
    11161 atccggtagt gcagggcggc gtattgctgg tggcgacgat gattatcctc gtcaacctgc
    11221 tggtcgatct gctgtacggc gtggtgaacc cgcgtattcg tcataagaag taaggggaca
    11281 tcatgtcaca ggttactgaa aataaagtga ttagcgcacc ggtgccgatg accccgttac
    11341 aggagttctg gcactatttt aaacgcaaca aaggcgcggt cgtcgggctg gtttacgtcg
    11401 tcatcgtgct gttcatcgcg atctttgcca actggattgc accctataac ccggcggaac
    11461 agttccgcga tgcactgctc gccccgccag cctggcagga aggcggcagc atggcgcact
    11521 tgctgggcac cgatgacgta ggccgtgatg tgctgtcgcg cctgatgtac ggtgcgcgcc
    11581 tgtcgctgct ggttggctgt ctggtagttg tgttatcgct gattatgggc gttattctcg
    11641 gcctgatcgc cggttacttt ggcggcctgg tcgataacat cattatgcgc gtggtcgata
    11701 tcatgctggc gctgccaagt ctgctgctgg cgctggtgct ggtggcaatt ttcggcccgt
    11761 cgattggtaa cgccgcgctg gcactgacct tcgttgcctt gccgcactat gtgcgcttaa
    11821 cccgcgccgc cgtgctggtg gaagttaacc gcgattacgt caccgcgtct cgcgtggcgg
    11881 gtgccggggc gatgcgtcag atgtttatta acatcttccc gaactgcctt gcgccgctga
    11941 ttgttcaggc gtcgctcggt ttctctaacg ccattctcga tatggctgct cttggtttcc
    12001 tcggcatggg ggcacagccg ccaacgccgg agtggggcac catgctctcc gacgtgttgc
    12061 agttcgcgca aagcgcctgg tgggtcgtga ccttcccggg tctggcgatc ctgctgacgg
    12121 tgctggcatt taacctgatg ggtgacggtc tgcgtgacgc gctcgatccc aaactgaagc
    12181 agtaagaggt tcgagatggc gttattaaat gtagataaat tatcggtgca tttcggcgac
    12241 gaaagcgcac cgttccgcgc cgtagaccgc atcagctaca gcgtaaaaca gggcgaagtg
    12301 gttgggattg tgggtgagtc cggctccggt aagtcggtca gttccctggc gattatgggg
    12361 ctgattgatt atccgggccg cgtaatggcg gaaaaactgg tgtttaacgg tcaggatttg
    12421 cagcgtatct cggaaaaaga gcgccgcaac ctggtgggtg ccgaagtggc gatgatcttc
    12481 caggacccga tgaccagcct taacccgtgc tacaccgtgg gtttccagat tatggaagcg
    12541 attaaggtgc atcagggcgg caacaaaagt acccgccgtc agcgggcgat tgacctgctg
    12601 aatcaggtcg gtattcccga tccggcttcg cgtctggatg tttacccgca tcagctttcc
    12661 ggcggcatga gccagcgcgt gatgatcgcc atggcgattg cctgtcgacc aaaactgctg
    12721 attgccgatg agccgaccac cgcgctggac gtgaccattc aggcgcaaat catcgaactg
    12781 ttgctggagc tacagcagaa agagaacatg gcgctggtgt taattaccca tgacctggcg
    12841 ctggtggcgg aagcggcaca taaaatcatc gtgatgtatg caggccaggt ggtggaaacg
    12901 ggcgatgcgc atgccatctt ccatgcgccg cgtcacccgt atactcaggc attgctgcgt
    12961 gcgctgccgg aatttgctca ggacaaagaa cgtctggcat cgttgcctgg cgtcgttccc
    13021 ggcaagtacg atcgcccgaa cggctgcctg cttaacccgc gctgccccta tgccactgac
    13081 agatgtcgcg ctgaagaacc ggcgctgaat atgctcgctg acgggcgtca gtccaaatgc
    13141 cattacccac ttgatgatgc cgggaggccc acactatgag tacgcaagag gccacctcgc
    13201 aacaaccgct gttgcaggcg attgacctga aaaaacatta cccggtgaag aaagggatgt
    13261 ttgcgccgga acgtctggtg aaggcgctgg acggcgtttc gtttaacctt gaacgtggca
    13321 aaacgctggc ggtagtgggc gaatctggct gcggtaaatc gaccctcggt cggttgctga
    13381 cgatgatcga aacgcccacc ggcggtgagc tgtattacca ggggcaggat ctgcttaagc
    13441 acgatccgca ggcgcagaag ctgcgtcggc agaaaatcca gatcgtcttc cagaatccct
    13501 acggttcgct gaatccgcgt aaaaaagttg ggcaaattct cgaagagccg ctgcttatta
    13561 acaccagctt aagcaaagaa cagcgtcggg aaaaagccct gtcgatgatg gcgaaagtcg
    13621 gcctgaaaac cgagcattac gaccgctatc cgcacatgtt ttccggcgga caacgtcagc
    13681 gtatcgctat tgcccgtggt ctgatgctcg acccggacgt ggtgattgcc gatgaaccgg
    13741 tttctgcgct ggacgtctcg gtgcgcgcgc aggtgctcaa cctgatgatg gatttgcagc
    13801 aggaactggg gctttcttat gtctttatct cgcacgacct gtcggttgtg gagcacattg
    13861 ccgatgaagt gatggtgatg tacttgggcc gctgcgtgga gaaggggacg aaggaccaaa
    13921 tcttcaataa cccgcgtcat ccttacactc aggcgctgct ctccgcgacg ccgcgcctga
    13981 acccggacga tcgccgcgag cgcatcaagc tcaccggtga actgccaagc ccgctgaatc
    14041 ctccgccggg ttgcgccttc aacgcccgct gtcgtcgacg cttcggcccc tgcacccagt
    14101 tgcagccagc actgaaagat tacggcggtc aactggtggc ctgctttgcc gttgatcagg
    14161 atgaaaatcc gcagcggtaa tgttcaaaac cggagccttg tgctccggtt tactcaatca
    14221 gcttcaacag tggcgacaag catagcaaaa tgccgtacag cagaatcagc caggctttaa
    14281 atccgcgaag tttttccagt tgtgcgactt tatagatcag gaaaaacgga atgaggcacg
    14341 acacaatgcc atataacggg cttcccaact gaaagaacac cagcaccgat acacgaaacg
    14401 aaacccaaat cgtcaacgta atgacgataa aagcgcagat cgccagagtc agcacgcgtg
    14461 agttaatttt cttggtatca ataatacggc ttaacagatt gagaataatg cctttaatgg
    14521 cctcgtggaa accgagataa atgccaaaga atgcggtcag tacggcaaag atattcagta
    14581 ccgtagaggt gatatgaatg atatgcccag ggatcacctg cgcggccagt gccagtgccg
    14641 agatattttg ttcaaaggca gaaacggctt cttcgtggct aattgagaag gtaaacgaaa
    14701 aggcaaaaaa cagtatcacc gcgatgagcg tgacataact aattcggtgg gtacgcagcg
    14761 cgagccgggt tgccagtact ttatccgctt cccgtttacg ataggcaata ttcattgggt
    14821 taagtacctg aataaatacc gcagaaaaga agcaaaaggg aatggtaagc aggacatcgc
    14881 ggaaaaagac ggaggcttgc gggaaggcgg ttatattggc gaaattccag tgcgggatca
    14941 tcgcaaaacc gaacacgaca ataatcccta ctttgaccac caccattggc ccagaaatct
    15001 taaacagtaa tcgttcacca ccagacgcaa tcgccaccag tacggcgaaa atagcgactt
    15061 tataaagtag agattgtgaa agatcggcat cggttaaacc gaaggttttc aggtacgagg
    15121 cgctgtcgaa aaccacggag agagagtaga taaaaatccc gtggataatc atcaaaaagt
    15181 agataacccc gaggaaaatt ccccagttct tccccaggta atgactgata atatcggtgt
    15241 agtcattaca ggaatcgctt tcagaaaggg tttttaaata aatgtcctgc actacccaag
    15301 tggcaggata agcaatgatc gctgcggtaa taaataccca aattcccttc aagccaattt
    15361 gtactggcat cagcacggtt ccggcaccaa tcgccatgcc tatgcataat aaaacccagc
    15421 cgaagtcgta gcgtgtaaac ggcaatttct gatcgtgttt cggtagtgtg ttgtgctgca
    15481 taaagtcctg tccagaaaga agtcggcctc gcaaaaatga ataaacccag attctcttgc
    15541 gcaacaggtg gagaagtcga atacgcagta tgtaaaaaat gaggcaacag attcactggg
    15601 gaggtcgcga ggaggcgcaa ttctacaaaa ttgcgcattt cactaccagt ctggagtcag
    15661 cccggatatg aactgcaact attgcaaatt aagaaattaa gctggaaggc atcacgcaat
    15721 gaggaagatt gccgggtgct gacagtaaaa agcaatcgta aaatcagcgg caagccggaa
    15781 gggttccggt gaggcgcaat gttgcggggg ctttatccct ggtggcattg gttgctggag
    15841 agagaaaacc cccgcacgtt gcaggtatat acctgacaac ataacggggg ctaatcttga
    15901 acctagacca ctcaagaata gccgcgaaac gttgtcatta caacacaggc ggctatatga
    15961 cgctcgcaga gctgggcatg gccttctggc atgatttagc ggctccggtc attgctggca
    16021 ttcttgccag tatgatcgtg aactggctga acaagcggaa gtagcgtgtc atgtgggcgt
    16081 caggctgccg taatggcaat ttgcgcccgg accaggccgc aggagtgaaa ctctgcggcc
    16141 tttttcgtaa gaaaaatgca acggttgcaa gcggataata ttctgcggaa tgcgcagcac
    16201 agagtgaaga tgagacggat tccggaacag aaaaacagtc gtagaatcag cgtcaagccg
    16261 gaaggattcc ggtgaggcgc aatgttgcgg gggcttaatc cctggtggca ttggttgctg
    16321 gagagagaaa acccccgcac gttgcaggta tgcacctgac aacaccacgg gggccattct
    16381 tgcatccaaa caatacaaga atagccgcga aacgttgtca ttacaataca ggcggctata
    16441 tgacgctcgc agaactgggc atggccttct ggcatgattt agcggctccg gtcattgctg
    16501 gtattcttgc cagtatgatc gtgaactggc tgaacaagcg gaagtaacgt gccatgcggg
    16561 cgtcaggctg ccgtaatggc aatttgcgcc cggatcaggc cgcaggggtg aaactctgcg
    16621 gcctttttcg taagaaaaat gcaacggttg caagcggata atattctgcg gaatgcgcag
    16681 cacagagtga gtaagtgatg gattccggaa cagaaaaaca gtcgtagaat cagcgtcaag
    16741 ccggaagggt tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg
    16801 ctggagagag aaaacccccg cacgttgcag gtatgcacct gacaacatca cgggggccat
    16861 tcttgaacct gaacaactca agaatagccg cgaaacgttg tcattacaac acaggcggct
    16921 atatgacgct cgcagaactg ggcatggcct tctggcatga tttagcggct ccggtcattg
    16981 ctggcattct tgccagtatg atcgtgaact ggctgaataa gcggaagtaa cgtgtcatgc
    17041 gggcgtcagg ctgccgtaat ggcaatttgc gcccggatca ggccgcagag gttaaactct
    17101 gcggcctttt tcgtgcttac tgcgggtaag gcacccagtc gccgccgttc aggcgaacgt
    17161 acggtttatc ctggtattga ataactactg catttgagtt ctcggagacc ggtgctgttt
    17221 gtggcaaccc actggtgagt tttttccagt caacattgtc ttcggtgaaa atcttgccat
    17281 caagaacgcg aaccaccaga tcggagatag ccaggaagct gctcggttgg tcaatgacaa
    17341 tcggtgcccc ctgatgtggt gccttcatgc cgaagaattt caccccaacg gggacgtctg
    17401 tgatagacgg gctagggata tcacgtaggc cagatacctg cattctgtcg cccttcagcg
    17461 cgccgccgtg ttccggcacc acgaccacca tcactttacg acccgatttc tccagttcag
    17521 taaagaaggc gtccagttca tcaaagaatt tctgcgcccg cgctttgtaa tccgctgttt
    17581 tgctgacccc cggataatgg ttgccgtcat gcagtggaag cgtgttgtag aacgtggcac
    17641 tacggctgtt tttatctttt tcggtaacgt ccagccagcg gttaagcacg gcggtatcgt
    17701 cataaaccgg cgaaccatca aagcccagca aaataaccgg cagatttgtt tgatccatca
    17761 attcagtctg catgccgcca ttttcgcgaa cttctttcaa aaaaccgccg aactggccgt
    17821 tatgccccat catcaggtgc tgggtaaagc ccagtttcga aaggttatca aacagatagc
    17881 agtcgttatt tgccggttga tacagattag tgtgcgaagt ctgcccgcag ctggcgcgca
    17941 gtaaacggat cgccgccggg ccactgtagg aggtggcgga gttaaagttc ttgaactcaa
    18001 tatcgaaatg cgaccacagt ggatgcgaca tcaacccggc ggcttctata tccgaccagg
    18061 aaagcgaaca gatgttaatc accagtagtt caaatggctg agcatcagcg ggcagcgaag
    18121 acgggaaggt cgatttacgt ttcgcctccg cgttatagaa attattcagc caggcgttaa
    18181 ggttcgccgt tgttggcggt gcagtttgtg cgggcatatc acccactacc ggtgcgccac
    18241 ccgtcgccgc aacggttgcc gctgcgttac cacccgtcgt tgttacagtg gtcgtcggtt
    18301 gtccggctgg ccacaaggag aaacttggtc ccgccagggt aagtacgttc agccatagca
    18361 gtatggcaac cacaaaaacg gtaatgcgaa tccattgtga caggaataac caggccacta
    18421 ataaaacaaa aatggcccca atcatctgcc agttaataaa gcgtgtgaca aggtcgatta
    18481 aataatcggt actgaacccc gccacctgcg aaccctggct cattatgctt tccgggccag
    18541 gcaaccaggt gtcatgccag aacaaagcaa agccgatcgg cagggcaatc cagtggcgca
    18601 agcgatgcag gctgtagcgc ggaatgggca tcagcagaaa cgcggcaaac accaaattga
    18661 ggagcggatg gaagttaaga tatcccgccc acaacaggcc gaacttaacc agaaaataga
    18721 agttccagcc ggaaaggccg cgccagtatt gccagaggga agaaggcatg gcggtatttt
    18781 gtgtaaattg agtcattttt tggttgccct ggctttttcc gtgcggcgta acgtcccggc
    18841 cggtttaaca taacgaggtt tagcaaagaa caaacgtaag gtgtcgcgaa tgcgtcgcaa
    18901 agagtgccgc gccagatagc ccagcgggaa aaatatcagt gcgcaaacga caataatttc
    18961 aatgatatcg ctgatggtca tcatgatgag cgctccacag catcatctaa cagtcgcatg
    19021 ggttctggta ttcgtcgcca gtggcgacca tcgtgctcgg cattgatgac cggtttagaa
    19081 ctttgcgtta aaggcagcgg catgccccat tgttctgggg caagcaagcg catctgcacc
    19141 agctcggcac tgatttgatc atcttcaaac cagaccatac ggtttgagaa aatgtcgcca
    19201 gtaggcaatg ggaaaatatg attcaacgcg gtatccagat cgttaatccg acagaatgag
    19261 agaaacagca ccagccgatt accgccaatg gtcatgatat cgccggtacg gttagggcga
    19321 cacagcgtca gggcttgttc aacgcggata cccggtaccg gacgtagggc aaccagaacg
    19381 cctttaccgt gggcaggcaa gagagggtta ttcatcatgt tgttgacggc attacagaac
    19441 acatcccact tctggaaacc acgcagtttg agcggctggg tcattgacag caaggtagtg
    19501 atatcttccg gtacatagcg actaaacttc tgcccttgca cgctttcgat catcgtcaga
    19561 caacgggaga gtggcgcatt ccacggaata accatatttg caccgcaggc caataacaaa
    19621 cgttcatcgg tggcgcgcag gctagcggta ttttcccgca cgaggatttt catcgcacta
    19681 ccgcgctggc gacgcagggt atgaatgctg cgggccagtg gctcgatttg cgcattttgc
    19741 tgtaaagaaa agaccaccgt cgccgcctga gcggtacggg cttcattgaa caggacttcg
    19801 ttattgttga acagttgcca gtgttccgat agcggcggcg caccttccag tacagcaaca
    19861 ttactcagaa tgcgtttttc gtcgctgcgt ggttggatct ccgcctcttc gctttgaact
    19921 aatgtccaga taccattttg ttgctgaacg ctaagctgct gacgggcgct gacccctttt
    19981 tcgttgcacc agaaggcaat atccagcaaa tgttggtcac cctgaaaacg caaactggca
    20041 agaccaaaaa gtgaacggta ttcctcaagc aacaatgaaa attgtttatc gttattattt
    20101 ccgggattaa ttaccaaaag cgaacaatgg tttaacctgc tccatttatt cattttatcc
    20161 aaccatgagc gaagccgctc ggcaggaatg ttttgccatg cgttatttgc gcaaacaaga
    20221 ataaaaaggt aattatgggg atcaatagaa cactgcaaat cacgggtcaa atagtataga
    20281 cctttttcat gatttagcat tgaaaataat tttatttttt ccggcccgtg agaatcatct
    20341 aattgaaaga tttttgccgg atcgctgtcc atgctaatga ccgcgacgtg tgcggtttca
    20401 gcctgggatg caattgtttg attcgccaga ctgatagcat cttcatggcg atcgacgtta
    20461 aaccaccaga cgccgcctgc tggcatatgt cgcagctcat cccataatga tgagataccg
    20521 atagagaata cagggtccac aatgtccctc atgtcaccat tttgtctgat tatcagttta
    20581 ctagcgaaag ctaagaaata aacctaccat tgaaattaaa acttatcaga tttagcatgt
    20641 gaatcaattt agaaggtcac aagacgtgtg attttagtct tttcgttttt aactttttct
    20701 gttgcgtaac ggaaagtcaa aaagtgagca aattcccgtc tcgccgtaaa caataaaccg
    20761 gcgaaatgct cacggttaga attgtctcat gaacggtaca gttatttcat agggatcaag
    20821 caaaatgaat aacaatgaac cagatactct gcctgatccc gcgataggct atatcttcca
    20881 gaatgatatt gtggcgttaa agcaggcatt ttcactgcct gatattgatt atgccgatat
    20941 ttcccaacgc gaacagttgg ccgcggcatt aaaacgctgg ccgttgctgg cagagtttgc
    21001 gcaacaaaag taggggattg gtgaatggcc gtactgggat tgcagggggt gcggggaggc
    21061 gtggggacaa caaccatcac cgccgcatta gcctggtcat tacaaatgtt gggagaaaat
    21121 gtcctggtgg tcgatgcctg cccggacaac ttgttgcgcc tgtcatttaa tgttgatttt
    21181 acccaccgtc agggctgggc cagagcgatg ctggatggcc aggactggcg tgacgctggg
    21241 ttgcgctaca cctcgcagct cgatttgctg ccttttggtc agttatccat tgaagaacaa
    21301 gaaaatccac agcactggca aacccggctg agcgatattt gctccggctt acagcaacta
    21361 aaagccagcg ggcgttacca gtggatttta atcgacttac cgcgtgatgc ctcgcagata
    21421 acccaccagc tgctgagttt gtgcgatcac tcgctggcaa tcgtcaatgt ggatgccaac
    21481 tgccatatcc gcctgcatca gcaagcgctg ccggatggcg cacatatttt gattaataac
    21541 ttccgtattg gcagtcaggt tcaggacgat atttaccagc tttggttgca aagccagcgc
    21601 cgattgctgc cgatgctcat tcatcgtgat gaagcgatgg ctgaatgcct ggcggctaag
    21661 caaccagtcg gtgaataccg cagtgatgcg ctggcggcag aagagatact gacgctggcg
    21721 aactggtgcc tgttgaacta ttccgggctg aaaacgccag tcgggagtgc atcatgagta
    21781 tcctgacccg gtggttgctt atcccgccgg tcaacgcgcg gcttatcggg cgttatcgcg
    21841 attatcgtcg ccacggtgcg tcggctttca gcgcgacgct cggctgtttc tggatgatcc
    21901 tggcctggat ttttattccg ctggagcacc cgcgctggca gcgtattcgc gcagaacata
    21961 aaaacctgta tccgcatatc aacgcctcgc gtccgcgtcc gctggacccg gtccgttatc
    22021 tcattcaaac atgctggtta ctgatcggta catcgcgcaa agaaacgccg aaaccgcgca
    22081 ggcgggcatt ttcaggtctg cagaatattc gtggacgtta ccatcaatgg atgaacgagc
    22141 tgcctgagcg cgttagccat aaaacacagc atcttgatga gaaaaaagag ctcggtcatt
    22201 tgagtgccgg ggcgcggcgg ttgatcctcg gtatcatcgt caccttctcg ctgattctgg
    22261 cgttaatctg cgttactcaa ccgtttaacc cgctggcgca gtttatcttc ctgatgctgc
    22321 tgtggggtgt agcgctgatc gtacggcgga tgccggggcg cttctcggcg ctaatgttga
    22381 ttgtgctgtc gctgaccgtt tcttgccgtt atatctggtg gcgttacacc tctacgctga
    22441 actgggacga tccggtcagc ctggtgtgcg ggcttattct gctcttcgct gaaacgtacg
    22501 cgtggattgt gctggtgctc ggctacttcc aggtcgtatg gccgctgaat cgtcagccgg
    22561 tgccattgcc gaaagatatg tcgctgtggc cgtcggtgga tatctttgtc ccgacttaca
    22621 acgaagatct caacgtggtg aaaaatacta tttacgcctc gctgggtatc gactggccga
    22681 aagacaagct gaacatctgg atcctcgatg acggcggtag ggaagagttt cgccagtttg
    22741 cgcaaaacgt gggggtgaaa tatatcgccc gtaccactca tgaacatgcg aaagcgggca
    22801 acatcaacaa tgcgctgaaa tatgccaaag gcgagttcgt gtcgattttc gactgcgacc
    22861 acgtaccaac gcgatcgttc ctgcaaatga ccatgggctg gttcctgaaa gagaaacagc
    22921 tggcgatgat gcagactcca caccacttct tctcgccaga cccgtttgaa cgcaacctgg
    22981 ggcgttttcg taaaacaccg aacgaaggca cgctgttcta tggtctggtg caggatggca
    23041 acgatatgtg ggacgccact ttcttctgcg gttcctgtgc ggtgattcgc cgtaagccgc
    23101 tggatgaaat tggcggcatt gctgtcgaaa ctgtgactga agatgcgcat acttctctgc
    23161 gtttgcaccg tcgtggatat acctccgcgt atatgcgtat tccgcaggcg gcggggctgg
    23221 cgaccgaaag tctgtcggcg catatcggtc agcgtattcg ctgggcgcgc gggatggtgc
    23281 aaatcttccg tctcgataac ccgctcaccg gtaaaggact gaagtttgct cagcggctgt
    23341 gctacgtcaa cgccatgttc cacttcttgt cgggcattcc acggctgatc ttcctgactg
    23401 cgccgctggc gttcctgctg cttcatgcct acatcatcta tgcgccagcg ttgatgatcg
    23461 ccctattcgt gctgccgcat atgatccatg ccagcctgac caactcgaag atccagggca
    23521 aatatcgcca ctctttctgg agtgaaatct acgaaacggt gctggcgtgg tatatcgcac
    23581 caccgacgct ggtggcgctg attaacccgc acaaaggcaa atttaacgtc accgccaaag
    23641 gtggactggt ggaagaagag tacgtcgact gggtgatctc gcggccctac atcttccttg
    23701 ttctactcaa cctggtgggc gttgcggtag gcatctggcg ctacttctat ggcccgccaa
    23761 ccgagatgct caccgtggtc gtcagtatgg tgtgggtgtt ctacaacctg attgttcttg
    23821 gcggcgcagt tgcggtatcg gtagaaagca aacaggtacg ccgatcgcac cgcgtggaga
    23881 tgacgatgcc cgcggcaatt gcccgcgaag atggtcacct cttctcgtgt accgttcagg
    23941 atttctccga cggtggtttg gggatcaaga tcaacggtca ggcgcagatt ctggaagggc
    24001 agaaagtgaa tctgttgctt aaacgcggtc agcaggaata cgtcttcccg acccaggtgg
    24061 cgcgcgtgat gggtaatgaa gttgggctga aattaatgcc gctcaccacc cagcaacata
    24121 tcgattttgt gcagtgtacg tttgcccgtg cggatacatg ggcgctctgg caggacagct
    24181 acccggaaga taagccgctg gaaagtctgc tggatattct gaagctcggc ttccgtggct
    24241 accgccatct ggcggagttt gcgccttctt cggtgaaggg catattccgt gtgctgactt
    24301 ctctggtttc ctgggttgta tcgtttattc cgcgccgccc ggagcggagc gaaacggcac
    24361 aaccatcgga tcaggctttg gctcaacaat gatgataacg cgatgaaaag aaaactattc
    24421 tggatttgtg cagtggctat ggggatgagt gcgttcccct ctttcatgac gcaggcgacg
    24481 ccagcaacgc aaccactgat caatgctgag ccagctgtag ccgcccagac ggaacaaaat
    24541 ccgcaggtgg ggcaagtgat gccgggcgtg cagggcgctg atgcgccagt cgtggcgcag
    24601 aacggtcctt cgcgtgatgt gaagctgacc tttgcgcaaa ttgcgccgcc gccgggcagc
    24661 atggtgctac gtggcattaa cccgaacggc agcattgagt ttggtatgcg cagcgatgaa
    24721 gtggtgacga aggcgatgct caacctcgaa tacacgccat cgccatcgtt actgcctgtc
    24781 cagtcgcagt taaaggttta tctcaatgat gagctgatgg gcgtgctgcc agtgaccaaa
    24841 gaacagttgg gtaaaaaaac gctggcgcaa atgcccatta acccactgtt tattaccgac
    24901 ttcaaccgtg tgcggctgga gtttgtcggc cattatcagg acgtgtgcga aaacccggcc
    24961 agcaccacgc tttggctgga tgttgggcgg agcagtggac tggatctgac ctatcagacc
    25021 ctgaatgtga agaatgacct gtcacacttc ccggtgccat tctttgatcc gcgtgataac
    25081 cgcaccaaca ccttgccgat ggtctttgcg ggtgcgccgg atgttgggct gcaacaagcc
    25141 tctgccattg tcgcctcgtg gtttggttcg cgttctggct ggcgtgggca gaacttcccg
    25201 gtgctctata accaactgcc ggatcgtaat gccattgtct ttgcaaccaa cgacaaacgg
    25261 ccggacttcc tgcgcgatca tccggcggta aaagccccgg tgattgagat gattaaccat
    25321 ccgcagaatc cttacgtcaa actgctggtg gtgtttggtc gtgacgacaa agacctgttg
    25381 caggcagcga aaggtatcgc tcagggtaac attctgttcc gtggtgaaag cgtggtagtg
    25441 aatgaagtga aaccgctgct accgcgtaag ccgtacgatg cgccgaactg ggtacgtacc
    25501 gatcgtccgg tcacatttgg cgaactgaaa acctatgaag aacagttaca atccagcggt
    25561 cttgagccag cagcgattaa cgtttcgcta aacctgccgc cggatctcta cctgatgcgc
    25621 agtaccggca ttgatatgga tattaattac cgctacacca tgccgccggt gaaagacagt
    25681 tcgcggatgg atatcagcct gaataaccag ttcctgcaat ccttcaacct gagcagcaaa
    25741 caggaggcga accgcctgct gctgcggatt ccggtattac aaggtttgct ggatggcaaa
    25801 acagatgtct ctattccggc gctgaaactg ggcgcgacca accagctgcg cttcgacttt
    25861 gagtatatga acccgatgcc gggcggttcg gtggataact gtattacctt ccagccggtg
    25921 cagaatcatg tggtgattgg tgacgactcc accatcgact tctcgaagta ttaccacttc
    25981 atcccgatgc cggatctacg cgcctttgct aacgcgggct tcccattcag ccggatggcg
    26041 gatctgtcgc aaaccatcac cgtgatgccg aaagcgccta acgaagcaca gatggaaacg
    26101 ttgctgaata ctgttggttt tatcggcgca cagacgggct tcccggcgat taatctgacg
    26161 gtgaccgatg atggcagcac cattcagggc aaagatgccg acatcatgat catcggtggt
    26221 atcccggaca aactgaaaga cgataagcag atcgacctat tggtgcaggc gaccgaaagc
    26281 tgggtgaaaa caccgatgcg ccagaccccg ttccccggca ttgtgccgga cgagagcgat
    26341 cgcgcggcag aaacccagtc aacgctgacc tcttccggtg cgatggcggc ggtgattggc
    26401 ttccagtcgc cgtataacga ccagcgcagc gtgattgcgc tgctggcaga tagcccacgc
    26461 ggttatgaaa tgcttaacga tgcggtgaac gatagcggca aacgcgccac catgttcggt
    26521 tcggtcgcgg tgatccgcga gtccggtatc aacagcctac gtgttggcga cgtttattac
    26581 gtaggtcatc tgccgtggtt cgagcgcttg tggtatgcgc tggcaaacca tccgattctg
    26641 ctggcggtgc tggcggctat cagtgtgata ttgctggcat gggtactgtg gcgtctgctg
    26701 cgaattatta gtcgtcgtcg tcttaacccg gataacgagt aattgaagat gaatgtgttg
    26761 cgtagtggaa tcgtgacgat gctgctgttg gctgccttta gtgttcaggc agcctgtacc
    26821 tggcctgcct gggagcagtt taaaaaggat tacatcagtc aggaagggcg cgtcatcgac
    26881 cccagcgacg cgcgcaaaat caccacctcc gaagggcaaa gttacggtat gttctttgcc
    26941 ctggcggcta acgaccgtgt agctttcgat aatattctcg actggacgca gaacaatctc
    27001 gctcagggtt ctttaaaaga acgtttgccc gcctggctgt ggggcaagaa agagaacagt
    27061 aagtgggaag tgctggacag caattcggcc tccgatggtg atgtctggat ggcctggtcg
    27121 ttgctggagg cggggcgttt gtggaaagag cagcgttata ccgacatcgg cagcgcgttg
    27181 ctaaaacgta tcgcgcggga ggaagtggtg acggtgcctg ggctgggttc catgttgtta
    27241 ccgggcaaag tgggttttgc tgaggataac agctggcgtt ttaaccccag ctacctgccg
    27301 ccgacgctgg cgcagtattt cacccgcttt ggcgcgccgt ggaccacgct gcgcgaaacc
    27361 aatcaacgtt tattgctgga aaccgccccg aaaggttttt cgccagactg ggtgcgctat
    27421 gagaaagaca aaggctggca gctaaaagcc gaaaaaacat tgatcagcag ctacgacgct
    27481 atccgcgttt acatgtgggt aggcatgatg cctgacagcg atccgcaaaa agcgcggatg
    27541 ctcaaccggt ttaaaccgat ggcgacattc actgagaaaa acggttatcc gccggaaaaa
    27601 gtggatgtgg ctacggggaa agcgcagggt aaaggaccgg tcggtttttc tgccgccatg
    27661 ctgccctttt tacaaaaccg cgatgcgcag gccgttcagc gccagcgcgt ggccgataac
    27721 tttcccggca gcgatgccta ttacaactat gtgctgaccc tgtttggaca aggctgggat
    27781 caacaccgtt tccgcttctc gacaaaaggt gagttattac ctgactgggg ccaggaatgc
    27841 gcaaattcac actaaacata ttcacgcttt ccctcggtct ggccgtcatg ccgatggtcg
    27901 aggcagcacc aaccgctcag caacagttgc tggagcaagt tcggttaggc gaagcgaccc
    27961 atcgtgaaga tctggtgcaa cagtcgttat atcggctgga acttattgat ccgaataacc
    28021 cggacgtcgt tgccgcccgt ttccgttctt tgttacgtca gggcgatatt gatggcgcgc
    28081 aaaaacagct cgatcggctg tcgcagttag cgccgagttc aaatgcgtat aaatcgtcgc
    28141 ggactacgat gctactttcc acgccggatg gtcgtcaggc actgcaacag gcacgattgc
    28201 aggcgacgac cggtcatgca gaagaagctg tggcgagtta caacaaactg ttcaacggtg
    28261 cgccgccgga aggtgacatt gctgtcgagt actggagtac ggtggcgaaa attccggctc
    28321 gccgtggcga agcgattaat cagttaaaac gcatcaatgc ggatgcaccg ggcaatacgg
    28381 gcctgcaaaa caatctggcg ctattgctgt ttagtagcga tcgccgtgac gaaggttttg
    28441 ccgtcctgga acagatggca aaatcgaacg ccgggcgcga aggggcctct aaaatctggt
    28501 acgggcagat taaagacatg cccgtcagtg atgccagtgt gtcggcgctg aaaaaatatc
    28561 tctcgatctt tagtgatggc gatagcgtgg cggctgcgca atcgcaactg gcagaacagc
    28621 aaaaacagct ggccgatcct gctttccgcg ctcgtgcgca aggtttagcg gcggtggact
    28681 ctggtatggc gggtaaagcc attcccgaac tacaacaggc ggtgcgggcg aacccgaaag
    28741 acagtgaagc tctgggggcg ctgggccagg cgtattctca gaaaggcgat cgcgccaatg
    28801 cagtggcgaa tctggaaaaa gccctcgcac tggacccgca cagcagcaac aacgacaaat
    28861 ggaacagtct gctgaaagta aaccgctact ggctggcgat ccagcagggc gatgctgcgc
    28921 tgaaagccaa taatcctgac cgggcagaac gcctgttcca gcaggcgcgt aatgtcgata
    28981 acaccgacag ttatgcagtg ctggggctgg gcgatgtggc gatggcgcga aaagattatc
    29041 ccgccgccga acgttattat cagcagacct tgcgtatgga cagcggcaac actaacgccg
    29101 tgcgcgggct ggcaaatatt taccgccagc aatcgccaga aaaagctgaa gcgtttatcg
    29161 cctcgctctc tgccagtcag cggcgtagca ttgatgatat cgaacgcagc ctgcaaaacg
    29221 accgtctggc acagcaggca gaggcactgg aaaaccaggg caaatgggcg caggcggcag
    29281 cacttcagcg gcaacgactg gcgctggacc ccggcagcgt atggattact taccgacttt
    29341 cgcaggatct ctggcaggcc ggacaacgca gccaggccga tacgttaatg cgcaatctgg
    29401 cgcagcagaa gccgaacgac ccggagcagg tttacgctta cgggctgtac ctctctggtc
    29461 atgaccagga cagagcggcg ctggcgcata tcaacagcct gccgcgcgcg cagtggaaca
    29521 gcaatattca ggagctggtt aatcgactgc aaagcgatca ggtgctggaa accgctaatc
    29581 gcctgcgaga aagcggcaaa gaagctgaag cggaagcgat gctgcgtcag caaccacctt
    29641 ccacgcgtat tgacctcacg ctggctgact gggcgcagca gcgacgtgat tacaccgccg
    29701 cccgcgctgc atatcagaat gtcctgacgc gggagccaac taacgccgat gccattctgg
    29761 ggctgacgga agtggatatt gctgccggtg acacagcggc ggcacgtagc cagctggcga
    29821 aactgcccgc taccgataac gcctcgctga acacacagcg gcgcgtggcg ctggcgcagg
    29881 cgcagcttgg agataccgca gcggcgcagc agacgtttaa taagttgatc ccgcaggcaa
    29941 aatctcagcc accgtcgatg gaaagcgcga tggtgctgcg cgacggtgcg aagtttgaag
    30001 cgcaggcggg cgatccaaag caggcgctgg aaacctacaa agacgccatg gtcgcatccg
    30061 gtgtgactac gacgcgtccg caggataacg acacattcac ccgactgacc cgtaacgacg
    30121 agaaagatga ctggctgaaa cgcggcgtgc gcagcgatgc ggcggacctc tatcgccagc
    30181 aggatcttaa cgtcaccctc gagcacgatt actggggttc gagcggcacc ggtggttact
    30241 ccgatctgaa agcgcacact accatgttgc aggtggatgc gccgtattct gacgggcgga
    30301 tgttctttcg cagtgatttc gtcaatatga acgtcggcag tttctccact aatgccgatg
    30361 gcaaatggga tgacaactgg ggcacctgta cattacagga ctgtagcggc aaccgcagcc
    30421 agtcggattc cggtgccagc gtggcggtcg gctggcgaaa tgacgtctgg agctgggata
    30481 tcggtaccac gccgatgggc ttcaacgtgg tggatgtggt cggcggcatc agttacagcg
    30541 atgatatcgg gccgctgggt tacaccgtta acgcccaccg tcggcccatc tccagttctt
    30601 tgctggcctt tggtgggcaa aaagactccc cgagcaatac cgggaaaaaa tggggtggcg
    30661 tacgtgccga cggtgtgggg ctaagtctga gctacgataa aggtgaagca aacggcgtct
    30721 gggcatcgct tagtgtcgac cagttaaccg gtaaaaatgt cgaagataac tggcgcgtgc
    30781 gctggatgac gggctattac tataaggtca ttaaccagaa caatcgccgc gtcacaatcg
    30841 gcctgaacaa catgatctgg cattacgaca aagatctgag tggctactca ctcggtcagg
    30901 gcggttacta cagtccgcag gaatacctgt cgtttgccat accggtgatg tggcgggagc
    30961 gcacggaaaa ctggtcgtgg gagctggggg cgtctggctc gtggtcgcat tcacgcacca
    31021 aaaccatgcc gcgttatccg ctgatgaatc tgatcccgac cgactggcag gaagaagctg
    31081 cgcggcaatc caacgatggc ggcagcagtc agggcttcgg ctacacggcg cgggcattac
    31141 ttgaacgacg tgttacttcc aactggtttg ttggcacggc aattgatatc cagcaggcga
    31201 aagattacgc acccagccat ttcctgctct acgtacgtta ttccgccgcc ggatggcagg
    31261 gtgacatgga tttaccgccg cagccgctga taccttacgc cgactggtaa gttttcagat
    31321 agcgcctctc ttaatgccgc tgcgatcggg tatactcggg cggcaatctg ggatttccgg
    31381 ggggagacaa tttgcgcgta agtcgctcgt taacaatcaa gcagatggca atggtggcag
    31441 ccgttgtcct ggtgttcgtt tttatttttt gcaccgtttt gctgttccat ctggtccagc
    31501 agaatcgcta taacacggct acgcaactgg aaagcattgc tcgctctgtc cgcgaaccct
    31561 tatcttcagc tattttgaaa ggcgatattc ccgaagcgga agctattctt gccagcatta
    31621 aaccggcagg cgtggtcagc cgtgccgatg tagtgctgcc taaccagttc caggcgctgc
    31681 gtaaaagttt tattccagag cgcccggtgc cggtaatggt tactcgcctg tttgagctac
    31741 cggttcaaat ctcgctgggc gtttactcgc tcgaacgtcc ggcaaacccg cagccaattg
    31801 cctatctggt actacaggcg gattccttcc gtatgtataa gttcgtgatg agcaccctct
    31861 caacgttagt gaccatttac ttacttttgt cgcttatcct gaccgtcgcc atcagctggt
    31921 gcattaaccg cctgattttg catccgttac gcaatattgc tcgcgaactt aacgccatcc
    31981 cagcccagga gcttgttggt caccaactgg cattaccgcg tctgcatcag gacgatgaaa
    32041 tcggtatgtt ggtgcgcagt tacaacctca accagcaatt gctgcagcgc cattatgaag
    32101 aacagaacga aaatgcgatg cgcttcccgg tgtcggattt gccgaacaaa gccttgctga
    32161 tggagatgct ggagcaggtt gtcgcgcgta aacaaaccac cgcgctgatg atcatcacct
    32221 gtgaaaccct gcgtgatact gcgggcgtgc tgaaagaggc gcaacgagaa attctgctgc
    32281 tgacgctggt ggaaaaactc aaatcggtac tgtcgccacg tatgatcctc gcgcagatta
    32341 gcggttatga ctttgctgtc attgccaacg gtgtacagga accgtggcac gcaatcacct
    32401 taggtcagca agtgctcact atcatgagcg agcgcctgcc gattgaacgt attcaactcc
    32461 gtccgcactg tagcattggc gtggcgatgt tctacggcga tctcaccgcc gaacagcttt
    32521 acagtcgcgc tatttctgcg gcatttaccg ctcgccataa aggcaagaat cagattcagt
    32581 tctttgatcc gcagcagatg gaagccgccc agaagcggtt gacggaagag agcgatatcc
    32641 ttaatgcact ggaaaatcat cagtttgcta tttggttaca gccacaggtc gagatgacca
    32701 gcggaaaact ggtcagtgcg gaagtgttac tgcgtatcca gcaaccggat ggcagttggg
    32761 acctgccgga tggcttaatc gatcgcattg agtgctgtgg gctgatggtt accgtcggtc
    32821 actgggtgct ggaagagtcc tgtcgattgc ttgcagcctg gcaagagcgc ggcattatgc
    32881 tgcccttgtc ggtaaacctc tctgcgctgc aactgatgca cccaaatatg gtggcggata
    32941 tgctggaact gttaacccgc tatcgcattc agccgggaac actgattctg gaagtgacag
    33001 aaagccgacg tattgacgac cctcatgctg cggtggcaat cctccgtccg ctgcgcaatg
    33061 ccggagttcg ggtggcgctg gatgatttcg gcatgggcta cgcagggctg cgtcagctgc
    33121 agcatatgaa atcgttgcca atcgacgtac tgaaaatcga caaaatgttt gttgaaggct
    33181 tgccggaaga tagcagcatg attgctgcaa ttatcatgct ggcgcagagc ctgaacttac
    33241 aaatgattgc cgaaggcgtg gagactgaag cacaacgcga ctggctggca aaagcgggcg
    33301 ttggtattgc ccagggcttc ctttttgctc gcccactccc tattgaaatc ttcgaagaga
    33361 gttacctgga agaaaagtag ctaccccaaa ctgattacaa aactttaaaa agtgctggtt
    33421 tgtgcgagcc agctcaaact ttttaacctt tttgtttcaa ttatgatcca ggtacatttc
    33481 tgtgatgttg tctgggtgtt attttaaggc cgcaggtacc ccataacctt acaagacctg
    33541 tggttttact aaaggacacc ctatgaaaac ctctctgttt aaaagccttt actttcaggt
    33601 cctgacagcg atagccattg gtattctcct tggccatttc tatcctgaaa taggcgagca
    33661 aatgaaaccg cttggcgacg gcttcgttaa gctcattaag atgatcatcg ctcctgtcat
    33721 cttttgtacc gtcgtaacgg gcattgcggg catggaaagc atgaaggcgg tcggtcgtac
    33781 cggcgcagtc gcactgcttt actttgaaat tgtcagtacc atcgcgctga ttattggtct
    33841 tatcatcgtt aacgtcgtgc agcctggtgc cggaatgaac gtcgatccgg caacgcttga
    33901 tgcgaaagcg gtagcggttt acgccgatca ggcgaaagac cagggcattg tcgccttcat
    33961 tatggatgtc atcccggcga gcgtcattgg cgcatttgcc agcggtaaca ttctgcaggt
    34021 gctgctgttt gccgtactgt ttggttttgc gctccaccgt ctgggcagca aaggccaact
    34081 gatttttaac gtcatcgaaa gtttctcgca ggtcatcttc ggcatcatca atatgatcat
    34141 gcgtctggcg cctattggtg cgttcggggc aatggcgttt accatcggta aatacggcgt
    34201 cggcacactg gtgcaactgg ggcagctgat tatctgtttc tacattacct gtatcctgtt
    34261 tgtggtgctg gtattgggtt caatcgctaa agcgactggt ttcagtatct tcaaatttat
    34321 ccgctacatc cgtgaagaac tgctgattgt actggggact tcatcttccg agtcggcgct
    34381 gccgcgtatg ctcgacaaga tggagaaact cggctgccgc aaatcggtgg tggggctggt
    34441 catcccgaca ggctactcgt ttaaccttga tggcacatcg atatacctga caatggcggc
    34501 ggtgtttatc gcccaggcca ctaacagtca gatggatatc gtccaccaaa tcacgctgtt
    34561 aatcgtgttg ctgctttctt ctaaaggggc ggcaggggta acgggtagtg gctttatcgt
    34621 gctggcggcg acgctctctg cggtgggcca tttgccggta gcgggtctgg cgctgatcct
    34681 cggtatcgac cgctttatgt cagaagctcg tgcgctgact aacctggtcg gtaacggcgt
    34741 agcgaccatt gtcgttgcta agtgggtgaa agaactggac cacaaaaaac tggacgatgt
    34801 gctgaataat cgtgcgccgg atggcaaaac gcacgaatta tcctcttaat ctcaccactt
    34861 atgcccgcac tcccttcccc gagtgcgggt gtttgcgcat aattgacccc tgtgcctgaa
    34921 cttaccacct gggtgagttc aggtttattt cacttcttcc gtgacatttt catgttcttg
    34981 cggtctaaca cgaagtgttt ttaacgtcat ttagactgac cgacaacggg cagttttttt
    35041 attaccagga tagttgatca ggggttcaca tgcagggcac aaaaattcga cttttagcgg
    35101 gcggtttgct gatgatggcc actgctggct atgtgcaggc agatgcgctc cagcctgatc
    35161 cagcatggca acaggggacg ctttccaacg gtttacagtg gcaagtgctg accacccccc
    35221 agcgtcccag cgatcgtgtt gaaattcgcc tgctggttaa taccggttcg ctcaccgaaa
    35281 gtacacaaca gagcggttac agtcacgcca tccctcgtat tgcgctaacg caaagcggtg
    35341 gccttgacgc agcacaggcg cgttcattgt ggcagcaggg gatcgaccct aaacgcccga
    35401 tgccgccggt aattgtctct tatgacacca cgctgtttaa tctgagtttg cccaataacc
    35461 gtaacgattt gctgaaagaa gcgctctctt atctggcaaa tgccactggc aaattgacca
    35521 tcacaccaga aaccatcaac cacgcgctgc aaagtcagga catggtggca acctggcctg
    35581 ccgatactaa agagggctgg tggcgctatc gtctgaaagg gtcaaccttg ttaggtcacg
    35641 atcctgccga tccgctgaaa caacccgttg aagcggaaaa aattaaagat ttctatcaga
    35701 aatggtacac cccggatgca atgacgctac tggtggtggg aaacgtggat gcgcgctcgg
    35761 ttgtcgacca aataaacaaa acgtttggcg aactgaaagg caaacgtgaa acgccagctc
    35821 cggtgccgac gctttctccg ctgcgtgcgg aagcggtgag tattatgact gacgcggtgc
    35881 gtcaggaccg gttatctatc atgtgggata cgccgtggca gccgattcgt gaatcagccg
    35941 cactgctgcg ctactggcgt gcggacctgg cccgcgaggc gctgttctgg catgttcagc
    36001 aagcgttaag cgccagtaac agcaaagaca tcggtcttgg atttgactgc cgtgtgctgt
    36061 atctgcgtgc gcagtgtgcc atcaacatcg aatcaccaaa cgacaagctg aacagcaacc
    36121 ttaatctggt ggcgcgtgaa ctggcgaagg ttcgcgataa aggtctgccg gaagaagagt
    36181 tcaatgcgtt agtggcgcaa aagaaactgg agctgcagaa actgtttgcc gcctatgcgc
    36241 gggctgatac cgatattctg atgggtcagc ggatgcgttc gttgcaaaat caggtcgtcg
    36301 atatcgcgcc ggagcagtat cagaaactgc ggcaggattt ccttaacagc ctgacggtgg
    36361 agatgttaaa tcaggatctg cgtcagcagt tgtcgaatga tatggcgtta atactgctgc
    36421 agccgaaagg cgagccggaa tttaacatga aagcgttgca ggcggcctgg gatcaaatca
    36481 tggccccatc gactgctgct gccgccacct ctgtcgccac ggatgacgta catcctgaag
    36541 tgacggatat tccacctgca cagtaatgcc tgatggtgat gagttagcac aaccatcagg
    36601 tatttatgga tgagctggat agaggggtaa cactttatcc agccttttgc atatgctgcg
    36661 tttacgctgg catcgcctca cgcgggataa tcgcgccgcg atactgaata acggtacttg
    36721 cggtcaggtg cccgcgtttc gccgcgtttt ccgcactgcc gcccgtcaaa cgtaccgcca
    36781 gataaccggc gctgaaagag tcgccagctg cggtggtatc gatcactttt tctttcggca
    36841 gtttcaccgc cggaacatcc actaaccctt cgccagcaat ggacaccagg caagaatccg
    36901 ccccgcgttt caccaccact tctttcacgc ccgcgttatg ggtgcgcgca atgacgtctt
    36961 ccaccggctg ttgaccccac agcgcgtctt catcgtccag cgtcaggaag gcgatatccg
    37021 tgcattccag catttgttgg tacacctgct gtgtctcttc tttgctggcc cacaggcgcg
    37081 gacgatagtt attgtcgaaa atcacttttc cgccgttggc gcggcattcg cgcagcaggg
    37141 aaagcagctt ttcgcggctg gtcgggctta agatcgccag gctaatcccg ctcaggtaga
    37201 gataatcgaa attcgccagc tcttcgcaaa tcgccgcaga ctgctcgctc tccagccaga
    37261 atttggcagc ggcttcgtta cgccagtagt agaacgtacg ttcaccggtg ctgtcggttt
    37321 caatgtagta aaggcccggc agacggtttt ccatccgttg ggtcagggaa gtatcaacgt
    37381 tctcgccgtg ccaggcgtcc agcatttgct ggctaaaact gtccgttccc agcgccgtta
    37441 cgtaatggac ggttaatgcc gcaggatcga cctgacgggc gatatagacg gaagtgttca
    37501 gggtatcgcc gccgaaaccg cgcttaacgt ccgcgccttt ctcggaaagc tcaatcatgc
    37561 attcgccaat cacggcaatc tttttggaca tagtcgtgaa cctgatctgt aaaaaattag
    37621 cgttagtttg cgctgtggtg gtttgctggt caaccatatt aaaacagtgt tccattattt
    37681 ttttgagcca ggacgtattc ctgaaagatt ccgttgtgga gtgaggaaat ttaccccggc
    37741 caggcggtaa agttctgccc ttacgcgccg ataatctttg tcgagtccgg gcagcatcac
    37801 ttttaaacac aggacatctt tgatgataag gcaggttatc cagcgaataa gcaaccctga
    37861 agcaagcatc gagagcttgc aggaacggcg tttttggttg cagtgtgagc gtgcttacac
    37921 ctggcagccg atctatcaaa catgcgggcg gttaatggcc gtggagctat taacggtggt
    37981 cgcgcatccc ttgaaccctt cgcaacgcct gccgccggat cgctatttta ctgaaatcac
    38041 cgtcagccat cggatggagg ttgtgaaaga gcagattgat ttgctggcgc aaaaagccga
    38101 cttctttata gagcacggcc tgctggcatc ggtcaatatt gatggcccta cgctcatcgc
    38161 cctgcgtcag caaccaaaaa tcctgcgcca gattgagcgt cttccctggc tgcgtttcga
    38221 actggtggag catatccgtc tgccgaaaga ttcaaccttt gcctcgatgt gtgaatttgg
    38281 cccgctgtgg ctggatgatt ttggtaccgg gatggcaaat ttctctgcac taagtgaagt
    38341 gcgttatgac tacatcaaaa tcgcgcgaga actgtttgtg atgctgcgtc agtcgccgga
    38401 aggacgcaca ctcttttctc agcttttaca tctaatgaat cgctattgtc gcggggtgat
    38461 tgttgagggc gtagaaacgc cggaagagtg gcgtgatgtt cagaactcgc ccgcattcgc
    38521 cgcacaaggc tggtttcttt cacgcccggc accgatagaa acgctgaata cggcggttct
    38581 ggcgctataa gctgcctcat tttccgcctg gctggactat ctttaggact ggcacaggaa
    38641 gaatatgagg aagcgaacga tgagcaaggc aggcaaaata accgctgcga tttcagtggc
    38701 tttcttgttg ttgattgtcg tggcgatcat tttgattgca acttttgact ggaatcgact
    38761 caaaccgacc atcaaccaga aagtctctgc ggagttgaat cgtccgttcg ctatccgtgg
    38821 cgatctgggc gtggtgtggg agcggcaaaa acaagaaact ggctggcgca gctgggtgcc
    38881 gtggccccat gtacacgcgg aagacatcat tcttggcaat ccaccggata ttcccgaagt
    38941 cacgatggtg catttgccac gcgtagaggc aacgctggcc ccgctggcgc tgctgaccaa
    39001 aacggtctgg ctgccgtgga tcaagctcga aaagcccgac gcgcgcctga ttcgcctctc
    39061 cgaaaagaac aataactgga cgtttaatct cgccaacgat gataacaaag acgcgaatgc
    39121 aaagccgtcg gcatggtcgt ttcggctgga taatattctt ttcgatcaag ggcggatcgc
    39181 tattgatgac aaagtaagca aagcggatct ggagattttt gttgatccgt taggcaagcc
    39241 gctgccgttc agcgaagtta ctggatcgaa aggtaaagcg gataaagaaa aggtgggcga
    39301 ttacgttttt ggcctgaagg cgcagggacg ttataacggt gaaccgctca cgggtacggg
    39361 aaaaataggc ggtatgctgg cgctgcgtgg cgaagggacg ccgtttccgg tacaggctga
    39421 tttccgctct ggtaacaccc gtgttgcttt tgatggcgtc gtgaatgacc caatgaagat
    39481 gggcggtgtc gatttacggc ttaaattttc tggcgattca ctgggtgatc tctatgaact
    39541 gacgggcgtt ctgctgcccg ataccccgcc gtttgaaacg gatggtcggc tggtagcgaa
    39601 agtcgacact gaaaaatcgt cggtctttga ttatcgcggt tttaatgggc gaattggcga
    39661 tagcgatatc cacggttctc tggtctacac caccggaaag ccacgaccaa aactggaagg
    39721 tgatgtcgag tcgcggcaat tgcggctggc ggacctggga ccgttaattg gcgttgactc
    39781 cgggaaaggg gcagaaaagt cgaaacggtc tgaacagaag aagggcgaaa aaagcgttca
    39841 gcctgcgggc aaagtgctgc cttatgaccg cttcgaaacc gataaatggg acgttatgga
    39901 tgccgatgtt cgcttcaaag ggcggcgcat tgagcatggc agtagcctgc cgattagcga
    39961 tctttctact catatcatcc tcaaaaatgc tgacctgcgc ctgcaaccgc tgaaatttgg
    40021 catggcgggc ggcagcattg cggcgaatat ccatctggaa ggcgataaaa agccgatgca
    40081 ggggcgggca gatattcagg ctcgtcgact gaaactgaaa gaactgatgc ccgatgtgga
    40141 actgatgcag aagacgctgg gggaaatgaa cggtgacgcg gaactacgcg gtagcggtaa
    40201 ctcggtggcg gcgcttttag gcaacagtaa cggcaacctg aaactgttga tgaatgacgg
    40261 gctggtgagc cgcaacctga tggagattgt tgggctgaat gtcggcaact acattgtcgg
    40321 tgcgatattt ggtgacgatg aggtgcgggt gaactgcgcg gcggcgaatc tgaatattgc
    40381 caacggcgtg gcgcgcccgc agatttttgc tttcgatact gagaacgcgt tgattaatgt
    40441 taccggcacg gcaagttttg cttcggaaca gctggatttg actattgatc cggagagtaa
    40501 agggattcgg attatcacac tgcgttcgcc gctgtatgtg cgtgggacgt ttaaaaatcc
    40561 gcaggctggg gtgaaagccg ggccgttgat tgcccgtggt gctgttgctg cggcactggc
    40621 aacgctggta acaccggcgg cggcgttact ggcactgatc tcaccttccg aaggggaggc
    40681 taatcagtgt cggacgatat tgtcgcagat gaagaagtga tgaggttgga ttgtgtttgc
    40741 tggtgagttc gggagcagcg tgttcccctc accctaaccc tctccccaaa ggggcgaggg
    40801 gactgttttg tgcgtgttga aacaacgagt accgcttttc cgttcacttt ctgggggagg
    40861 gctgttatgt tcgcgatata acatctcgct ccgcttttct ccctctccct ttcagggaga
    40921 gggctggggt gagggtggct gcccacacga tacttacaac gactgatgtc gcgtctcatg
    40981 ggtcagcagc agggcgatta acgtcaagcc agccatcgcc gccagatata accccaccgc
    41041 acctaacccg tagttagtct gcaaccaggc tgcgatatac ggcgcaacgg aagccccgag
    41101 aatcgacgct acgttgtaag agaacgatgc tccggtgtaa cgcacttctg ttgggaacag
    41161 ctctggtaac agcgcgccca tcggcccgaa ggtcagcccc atcagactta accccagcag
    41221 caggaaggca aaaaccagaa tcgggttgcc agaaccgagc agcgggttaa aggcgaacag
    41281 cgcgaacagg atgatcagcg tggtgataat taccatgctt ttacggcgac caaaggcatc
    41341 agccagtaat ccggcgactg gcaccatcac gccaaaacca ataactgcca tcatcagcat
    41401 ccacaacact tcgttacgtg gcaggccaag cccaactggc gcggcggcgg tactgaaggt
    41461 catagagtag accgtcatga tgtaaaacag cgtatatgtt gccagcatga tgaacgtacc
    41521 cagtacggtt acgcgaacat gtttggtcag cagcgtaccc agcgggatct tcacctgttt
    41581 tttcgcttta gcgactttct caaacaccgg cgactcatgc agcgacacgc gaacatacag
    41641 gccgataatg accagcaccg ccgagaagat aaatggcaca cgccagcccc agctcataaa
    41701 ctgctcatcg gtcagtagcc aggaaagcag caagaaagtg ccgttggcaa agaagaagcc
    41761 aatcggtgcg cccagctgtg ggaaggagcc atacagtgca cgtttgcgcg gtggggcgtt
    41821 ttcagtcgcc agcagcgccg cgccgcccca ttcaccgcct aagcccagac cctgaccaaa
    41881 tcgagccagc gccagcagca gtggggcgaa aataccaatc gtggcatagc ccggcagcag
    41941 accaatcacc acggtcgaaa tccccatcgt tagcaacgag gcgaccagcg tcgctttacg
    42001 cccaacgcga tcgccaaaat gaccaaaaac ggcagagcca atggggcgcg cgacgaaggc
    42061 gatggcgaag gtggcgagcg actgtagcgt tgctgccgtt ggatcgccct gcgggaagaa
    42121 gatatgcgga aacacaataa cggccgcagt ggcgtaaatg taaaagtcga agaactcaat
    42181 ggccgtgcca atgagagagg cgacaaggac tttattacgc gagttgatcg gcgtgtattc
    42241 ttgctcgtgg tcgagtgttg tggctgttgc ttgcataatc ttttcttatt ttttggctaa
    42301 cgaatagcca tattacgcac agcaaaagtg atatttcaat gtgtaacaaa tcgcggaaat
    42361 ggtcaaaaac caggcaaaaa ggggataatg tttaggccag tgatttttct tctatgaaat
    42421 gcttcacata attttataaa tagtggataa aactggtttc aggttaaata aaagttacca
    42481 actggattta tatgctgggg ttatgagttg gaatgaaatt gtttgctccc aaatagatgg
    42541 ctgaagaggc tctggggaag agtttcaaga ggttatgtgc gcattatagc cggatgcgat
    42601 gctggcgcat cttatccggc ctacggggag ccaggccgaa taaaatcgta tgccggataa
    42661 ggcgtttacg ccgcatccgg cctattttaa ggctgcgttt tccctggcat tcgcgggtcg
    42721 tctttatatt cggcggtggc aatccacgcc gcgcaaaaca gcgtcaaacg ggcgaagaag
    42781 tagaaaaatg ccatcagccc cagcacggaa ccaaatgctg cgccagaggg ggatttcatc
    42841 agcgatggca aggtgtaggt catcacgatt ttaatcactt caaaaccaat agcagccaga
    42901 aatgttccgc gaatcagggc ttttttgcgc ggacggtggc gcggcaaccg ccagaagatc
    42961 cagaagaaaa gcagatagtt ggcgaagatg gaaatcgcca ggccaatcaa ccgccacgtc
    43021 ggtttcagcc actcaatgct gttcaggtgc agggcgctaa taatcatttg ctgcgccgaa
    43081 ccggcaaccg aggtgatcga aagcgtcaca atcagcgcaa tcaacaaacc aatcagcgaa
    43141 ataaaatcac gcagatattt tacccagaac ttctcctgat cttgcggcga gcgttcccag
    43201 acatcgcgcg actgggcacg aatcgcttca cgcaggttac ccatccagtt gatgccggaa
    43261 taaagcgcca ccgccaggcc gacaagccct acagtcgtac gctgctgaac ggcggtgttg
    43321 atggtgtttt tcaacgtggc ggctagcgtc ggatcgctga tgttttgcag aattttgtcg
    43381 aagatatcct gtagcaacat cggatgggag gccagcacaa agccccctgc ggcaaacgac
    43441 accatcaaaa tcgggatcat cgataaaaat gagaaatagg tgatagccgc accaaactgg
    43501 ttacccagcc gatcgttaaa gcgttctgtc gcgcgaatca ggtgcgcaat aaccggttgg
    43561 cgttggactt tttcggcagt ggtggtgacg gtttccagcg cctggctaac tttgccgcct
    43621 ttctcgctat tatccaactg cttaatcggc tcgtgctcca gatcctgggt gggacgtttg
    43681 atctcgtttt cctgcgtcat ccgctctttt gtccttctgt cgtggtcatt cttaaaaagt
    43741 atagtcggtc agtccaggtg ctctttcatt acgccagcca gccactgcat aaacaggttt
    43801 acacgccggg aaagctcccg acgctgcggg taaaccagcg aaagggagag cggctcggca
    43861 cggtagcctg gcaacacttc aataagccgc ccggcacgca gggcttcgcg cacggcaatg
    43921 cgcgggatct gaataatccc cagaccggca agaccggcgg cgagatagtt ttcgctactg
    43981 tttaccgtca acatgccgcc agacttaaac cactggacgc cattaacgct ggcaacctca
    44041 aaacctaacg gatgtacacc caggtgcggt gtataacgca ctattgcgtg tgaagtcaga
    44101 tcatcggggc tttgcggata accaaagcgc gtcagatagt gcggactggc acagttgacc
    44161 atggtcagtt tgccgagggg acgggcgata acgccatctt ccggtaacgc gccagtgcgt
    44221 atcacgcaat caaaaccatc atgaagaata tctaccggac ggtcatggct actcagttcc
    44281 agctcaattc ccggatgcag atagagaaat tccgacaggc gcggcagtaa caggcttttc
    44341 gcgattcctg gcgggatgtc gatgcgtaat ttaccgctga tactggtggc atcctgttga
    44401 aacagaccgt ccagttcgct gagattactc aacacatctt ttgctcgttg ataataggtc
    44461 atgccttctg gcgtgagttt gacccgtcgc gtggtgcgct ggagaagctg ggtgccaagt
    44521 tgatgttcca gtgcctgtat ctggcgcgaa acacttcctt ttggcaaagc aaagaaatcc
    44581 gccgcgcggg aaaaactttc cagctccgcg actttgatga acaactgcat tgcgtgaatt
    44641 ttatccattt ctttgaccga ttgttgttca cagcgaaaca gtaacgcgca tatcgcattg
    44701 tttattgttt tttctaacat ctaatgccgg gtttcacaat gttgtcatga cgaatgtggc
    44761 gatttctcag caggaaaagg gcggaatagc tttctgagaa aggtgaatcc ccctaaacag
    44821 agcgtatttt tcagcgattc cctttaaagt gaatagagga cgtgttggct atttgtgctt
    44881 atttttgtct cattgccgtt tgttattttt gcttgtggtc gcggagtttc atttttaatt
    44941 gcatgcgtac taaccaccaa tataaggaaa tcctcaaaat tgttttaaat agtgccctcg
    45001 acatgtaatt tcaggcgttt agctgtaact atttatatcg gtaactattt tctacgcgtc
    45061 atctctgtag aatggccacc gccaaatggc gaattcttat tttttctcgc gggtaatacc
    45121 cttccactac ttaagtcttt ttaacgtatt caggtggaga ttcagttatg caaatagtca
    45181 tgtttgacag gcagtcaata tttattcatg gaatgaaaat cagtttacag cagcgtattc
    45241 caggagtgag tattcagggg gccagtcagg cagacgagtt atggcaaaag ctggaaagtt
    45301 accctgaagc cttagttatg ctcgatggcg atcaggatgg tgagttttgc tactggttgc
    45361 tgcaaaaaac cgtggtgcag tttcctgagg ttaaggtgtt aatcacggcg acggattgca
    45421 ataaacggtg gttacaggaa gttatccatt ttaatgtgct ggccattgtg cctcgtgatt
    45481 caaccgtcga gacatttgcg ttggcggtaa atagtgcggc gatggggatg atgtttctgc
    45541 ctggagactg gcggatgacg ccggaaaagg acattaaaga tctcaaatcg ttaagcgccc
    45601 gccagcggga gattttaacc atgttagcgg cgggcgaatc aaataaggag atcggcagag
    45661 cgctgaatat cagtaccgga acggtaaaag cgcatctgga atccctgtat cgccgtctgg
    45721 aagtgaaaaa tcgcacccag gcggcaatga tgttaaatat ctcctcctga agaggataat
    45781 tttacagcca gcaggaagtt agcggctggc tgtaaaaata ttatggttcg ccgtacaaac
    45841 caattaaacg gcgtaccaca ccgttagtcc agccaaaccc atcctgcaac ggatactcgc
    45901 caccgccgcc ttcgcgggga acaccatcgg caatatggta tttctcgatc attttgtgct
    45961 gttccagata gaactgattc accgttttca gccagctgcg cgcgatttca tcacccagaa
    46021 ggtcatcgcc atacatttta aatccctgaa ttgccatcca ttgtaacggt gcccagccat
    46081 tgggtttatc ccactgttca ccggtttcgt actcgcttgc cagaatcccg ccaggtgtca
    46141 gtaaccggct gcgtacggcg tttgccagac gatcggcctg ttcatggttc gccatgccga
    46201 catagagcgg cacaatggcg gcagcggaaa acagcgccag ttgttcgcgt cgccagtcgt
    46261 aatcgcggta gatgccgttt tcatcatccc agaggtaacg gtttaccgca tcgcgacggg
    46321 cactggcctt ctggcggaac agcgcttctg tctctttctc gcctttcagc gccgagatgt
    46381 tggcgatggc gctctccagt ttaaacagga aggcattcag atcgatgggg atgaactggg
    46441 tggtacgaat gctcgccaga cgaccagtat cacgcagcca acgggaagag taatcccaac
    46501 ctgaggccgc tcccgcgcgt aaatcgcggt acacctcgtt gggcgggcga ccagaatgtt
    46561 tcgcggtttc aacgtcctca agccaggatt cgtcacgcgg cgtgtcgcga tcgtcccaat
    46621 aacggttgag cagcgatccg tccggcatcc gcacaacatg gcgataggcc tgattaggga
    46681 tcaacgattc tgcaccgtcc atccagaagg catattccat tttaaggtgg tcgagatagc
    46741 ggcgcgcacc gcgtacacca tcttcttcaa acaactccac catcagcgca aaaaccggtg
    46801 gttgcgatcg gctcaaataa taggtgcggt tgccgttggg gatgtgacca tagttttcga
    46861 tcatccaggc gaagttatcg gccatgcatt tcagcaaatc ttcccgacca ctttccgcca
    46921 gccccagcat ggtgaaatag gaatcccaat agtaggtttc gctaaaacgg ccgcccggga
    46981 caatatatga ctgcggtagc gccagcagag aagaccacgg aatgtgatcc tgtggttcgc
    47041 gggttagcac cggccacagc tggtcgatat gctctttcag ggaattttgc gggtccgata
    47101 catactctct ggagtagacc tccggcagcc agaagtgatt ttcaacaaac ttgcgcaagt
    47161 caaaatcacg atgacggcgc actttacggt agcggattaa aatatccagc gggtccattt
    47221 tgggtgcgca gtcgggaaag gttttactgt cggggaaaat tttcgccgac tgaacatgct
    47281 cgaacagttc gagatagcga tcggcaggcg tcagcgcatc agaggcgggc agcccttcaa
    47341 tcatctcggg ttccggttct gcctcgatca tctcatccag ttttaattca tacgggtcca
    47401 gctcatagca gagatcgact tcgatcatca gttcgtctgg attagggttt tgaattttct
    47461 gattgagcat aaggatccaa cctccgaaat tcgtcgctaa ttatcgggct tttctttaag
    47521 ggtagtcatt cgctccgcct ggtgggcgac aaagcgtgcg gtggatcacg tttattaacc
    47581 tgtcaaaaaa tataacgcac taatatccat ggaatttata aaaatacgga tttcgtgttg
    47641 tttttcattt tcagaacatt caatcctgct ctcgccgcaa tcatgatatg tcattcctga
    47701 tctgggtaat aggcacaggc tatcttattg atagtttata ttcatgtaat tgattgattc
    47761 ctatcaaaaa gcgtatctcc ctgtttttta tgatggcttt gtctcgcaga gacgtttgca
    47821 gcattctgga aaattttctg ggtcacaata actcaccacc gtgagggaag aataatgaaa
    47881 atggtctcac gtattaccgc gatcggcctg gctggcgtcg cgatttgcta tttagggtta
    47941 tctggttatg tgtggtacca cgataataaa cgcagtaaac aggccgatgt tcaggcatct
    48001 gctgtcagtg aaaataataa ggttttaggc tttctccgcg aaaaaggatg cgactattgc
    48061 cacacacctt cggcagaatt acccgcctat tattatattc ctggcgcgaa acagttgatg
    48121 gattacgaca ttaagcttgg atataaatct tttaacctcg aggccgtgcg tgcggcactg
    48181 ctggctgata aacccgtttc gcaaagcgat ctgaataaga ttgaatgggt gatgcagtat
    48241 gaaactatgc caccaacgcg ttataccgcg ctacactggg cgggtaaggt gagtgatgaa
    48301 gagcgggcgg aaatactggc ctggattgca aaacagcgcg cggaatatta cgccagcaat
    48361 gatactgctc cggagcatcg caatgaaccg gtgcagccca tcccgcaaaa actgcctacc
    48421 gatgcgcaaa aagtggcgtt gggctttgcg ctgtatcacg atccccgttt atcggctgat
    48481 agcaccattt catgcgctca ttgccatgcg ttgaatgcgg ggggcgtcga tggcagaaaa
    48541 acatcgattg gtgttggtgg cgcagttggg ccgattaacg cgccgacggt atttaactca
    48601 gtatttaacg ttgagcagtt ctgggatggt cgtgcggcaa cattgcagga tcaggcaggt
    48661 ggaccgccgt tgaacccgat tgaaatggcg tcgaaatcct gggacgaaat tattgctaag
    48721 ctggaaaaag atccgcagct taaagcgcag ttcctcgaag tttatccgca aggtttcagt
    48781 ggcgaaaata ttactgatgc cattgctgaa tttgagaaaa cattaattac gccggattcc
    48841 ccatttgata aatggttgcg tggagatgaa aatgcgctga cggcgcaaca gaaaaaaggc
    48901 tatcaattat ttaaagataa taaatgtgca acttgtcatg gtggtattat tctcggcgga
    48961 cgttcctttg aaccgttggg gctgaaaaaa gactttaact ttggggaaat tacggcggcg
    49021 gatattggtc gtatgaatgt gactaaagaa gagcgtgata aattgcgtca gaaagtaccc
    49081 ggtttacgta acgttgcttt aacggcaccg tacttccatc gcggtgacgt gccgacgctg
    49141 gacggggcgg tgaaactgat gctgcgctat caggtaggca aagagctgcc gcaggaggat
    49201 gtggatgata tcgtagcttt cctgcacagt ctgaacgggg tgtacacgcc gtatatgcag
    49261 gataaacaat aattaatttg atcgcccgaa caggaatgtt tgggcgattt ttattacgat
    49321 aataaagtct g
//
LOCUS       DAPHDW010000018        49148 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.018, whole genome shotgun sequence.
ACCESSION   DAPHDW010000018 DAPHDW010000000
VERSION     DAPHDW010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 49148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49148
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.018"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(182..1129)
                     /gene="dusC"
                     /locus_tag="RH057_001001"
     CDS             complement(182..1129)
                     /gene="dusC"
                     /locus_tag="RH057_001001"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HDV3127411.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGSWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCIAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATGLFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            1368..1766
                     /locus_tag="RH057_001002"
     CDS             1368..1766
                     /locus_tag="RH057_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HDV3127412.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            1763..2458
                     /locus_tag="RH057_001003"
     CDS             1763..2458
                     /locus_tag="RH057_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HDV3127413.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            2588..3472
                     /gene="cdd"
                     /locus_tag="RH057_001004"
     CDS             2588..3472
                     /gene="cdd"
                     /locus_tag="RH057_001004"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HDV3127414.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            3622..4341
                     /gene="sanA"
                     /locus_tag="RH057_001005"
     CDS             3622..4341
                     /gene="sanA"
                     /locus_tag="RH057_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HDV3127415.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPTDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            4344..4583
                     /locus_tag="RH057_001006"
     CDS             4344..4583
                     /locus_tag="RH057_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HDV3127416.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     IWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            4902..6140
                     /gene="preT"
                     /locus_tag="RH057_001007"
     CDS             4902..6140
                     /gene="preT"
                     /locus_tag="RH057_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HDV3127417.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDTPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEVGNTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLPGELT
                     MAADKIILAVGQHARLDAFAELEPQRNTIKTQNYQTRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            6134..7369
                     /gene="preA"
                     /locus_tag="RH057_001008"
     CDS             6134..7369
                     /gene="preA"
                     /locus_tag="RH057_001008"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HDV3127418.1"
                     /translation="MLTKDLSITFCGVKFPNPFCLSSSPVGNCYEMCAKAYDTGWGGV
                     VFKTIGFFIANEVSPRFDHLVKEDTGFIGFKNMEQIAEHPLEENLAALRQLKEDYPDK
                     VLIASIMGENEQQWEELARLVQEAGADMIECNFSCPQMTSHAMGSDVGQSPELVEKYC
                     RAVKRGSTLPMLAKMTPNIGDMCEVALAAKRGGADGIAAINTVKSITNIDLNQKIGMP
                     IVNGKSSISGYSGKAVKPIALRFIQQMRTHPELCDFPISGIGGIETWEDAAEFLLLGA
                     ATLQVTTGIMQYGYRIVEDMASGLSHYLADQGFDSLQEMVGLANNNIVPAEDLDRSYI
                     VYPRINLDKCVGCGRCYISCYDGGHQAMEWSEKTRTPHCNTEKCVGCLLCGHVCPVGC
                     IELGEVKFKKGEKEHPVTL"
     gene            complement(7440..8450)
                     /gene="mglC"
                     /locus_tag="RH057_001009"
     CDS             complement(7440..8450)
                     /gene="mglC"
                     /locus_tag="RH057_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HDV3127419.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKVSGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(8466..9986)
                     /gene="mglA"
                     /locus_tag="RH057_001010"
     CDS             complement(8466..9986)
                     /gene="mglA"
                     /locus_tag="RH057_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HDV3127420.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            complement(10047..11045)
                     /gene="mglB"
                     /locus_tag="RH057_001011"
     CDS             complement(10047..11045)
                     /gene="mglB"
                     /locus_tag="RH057_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HDV3127421.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            complement(11325..12365)
                     /gene="galS"
                     /locus_tag="RH057_001012"
     CDS             complement(11325..12365)
                     /gene="galS"
                     /locus_tag="RH057_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HDV3127422.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AATITNSTNQAM"
     gene            complement(12507..13664)
                     /locus_tag="RH057_001013"
     CDS             complement(12507..13664)
                     /locus_tag="RH057_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HDV3127423.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYGAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLICWRLVRDAPSVKSLFNTGVMLYLVGLGVLLLLGLISDSQTSRA
                     WTPDASAILYEKYWKLHGGVDAISNRADGVGNSLLALGAQYGWQLAGMMLIGAALMRS
                     GWLKGQFSLRHYRRTGFVLVAIGVTINLPAIALQWQLDWAYRWCAFLLQMPRELSAPF
                     QAIGYASLFYGFWPQLSRFKLVLAIACVGRMALTNYLLQTLICTTLFYHLGLFMHFDR
                     LELLAFVIPVWLANILFSVIWLRYFRQGPVEWLWRQLTLRAAGTAISKTSR"
     gene            complement(13681..14349)
                     /gene="folE"
                     /locus_tag="RH057_001014"
     CDS             complement(13681..14349)
                     /gene="folE"
                     /locus_tag="RH057_001014"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HDV3127424.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            14607..15443
                     /gene="yeiG"
                     /locus_tag="RH057_001015"
     CDS             14607..15443
                     /gene="yeiG"
                     /locus_tag="RH057_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
                     /protein_id="HDV3127425.1"
                     /translation="MEMLEEHRCFEGWQQRWRHDSSTLNCPMTFSIFLPPPRDHTPPP
                     VLYWLSGLTCNDENFTTKAGAQRVAAELGIVLVMPDTSPRGEKVANDDGYDLGQGAGF
                     YLNATQPPWATHYRMYDYLRDELPALVQSQFNVSDRCAISGHSMGGHGALIMALKNPG
                     KYTSVSAFAPIVNPCSVPWGIKAFSSYLGEDKNAWLEWDSCALMYASNAQDAIPTLID
                     QGDNDQFLADQLQPAVLAEAARQKAWPMTLRIQPGYDHSYYFIASFIEDHLRFHAQYL
                     LK"
     gene            complement(15475..17548)
                     /gene="cirA"
                     /locus_tag="RH057_001016"
                     /pseudo
     CDS             complement(15475..17548)
                     /gene="cirA"
                     /locus_tag="RH057_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(17841..19310)
                     /gene="lysP"
                     /locus_tag="RH057_001017"
     CDS             complement(17841..19310)
                     /gene="lysP"
                     /locus_tag="RH057_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HDV3127426.1"
                     /translation="MVSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            complement(19515..20396)
                     /locus_tag="RH057_001018"
     CDS             complement(19515..20396)
                     /locus_tag="RH057_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3127427.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTREIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            20495..21544
                     /locus_tag="RH057_001019"
     CDS             20495..21544
                     /locus_tag="RH057_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HDV3127428.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            21618..22475
                     /gene="nfo"
                     /locus_tag="RH057_001020"
     CDS             21618..22475
                     /gene="nfo"
                     /locus_tag="RH057_001020"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HDV3127429.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQDDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            22479..23567
                     /locus_tag="RH057_001021"
     CDS             22479..23567
                     /locus_tag="RH057_001021"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3127430.1"
                     /translation="MNDREKQILKILRRNPLIQQNEIADILQISRSRVAAHIMDLMRK
                     GLIKGKGYILTEQDYCVVVGAINMDIRGMADIRYPQAASHPGSVHCSAGGVGRNIAHN
                     LALLGRDVHLISAIGNDFYGETLLEETRRAGVNVSNCIRLHGHSTATYLAIANKQEET
                     ILAINDTHILQQLTPQLLNTSRDLIRHAGVVLADCNLTPEALEWVFTIADEIPMFVDT
                     VSEFKANKVKNWYSRIHTLKPTQNELEILWGQPIKDDNDRIRAVNSLHQQGVKRIFVY
                     LKDESVFCSDKDGEQFLLTAPAHTTVDSFGADDGFMAGLVYSFLEGYSFRDSARFAVA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(23623..24873)
                     /locus_tag="RH057_001022"
     CDS             complement(23623..24873)
                     /locus_tag="RH057_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV3127431.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEAAATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFAHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTGGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(24973..25914)
                     /gene="rihB"
                     /locus_tag="RH057_001023"
     CDS             complement(24973..25914)
                     /gene="rihB"
                     /locus_tag="RH057_001023"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HDV3127432.1"
                     /translation="MEKRKIILDCDPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRQQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERAGGPAGELFSD
                     IMNFTLKTQFENYGLAGGPVHDATCIGYLINPDGIKTQEMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            26044..26742
                     /gene="yeiL"
                     /locus_tag="RH057_001024"
     CDS             26044..26742
                     /gene="yeiL"
                     /locus_tag="RH057_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
                     /protein_id="HDV3127433.1"
                     /translation="MKEIHNNDLKQQLMSESAFKDCFLTDVSADTRLFHFLARDYIVQ
                     EGQQPSWLFYLTRGRARLYATLANGRVSLIDFFAAPCFIGEIELIDKDHEPRAVQAIE
                     ECWCLALPMKHYRPRLLNDTLFLRKLCVTLSHKNYRNIVSLTQNQSFPLVNRLAAFIL
                     LSQEGDLYHEKHTQAAEYLGVSYRHLLYVLAQFIHDGLLIKSKKGYLIKNRKQLSGLA
                     LEMDPENKFSGMMQ"
     gene            complement(26810..28060)
                     /locus_tag="RH057_001025"
     CDS             complement(26810..28060)
                     /locus_tag="RH057_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HDV3127434.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRMNRNELFTAICSGMASIAGSMMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLVGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTIAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(28154..29092)
                     /gene="psuG"
                     /locus_tag="RH057_001026"
     CDS             complement(28154..29092)
                     /gene="psuG"
                     /locus_tag="RH057_001026"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HDV3127435.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEEIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNITV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTGGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            complement(29080..30021)
                     /locus_tag="RH057_001027"
     CDS             complement(29080..30021)
                     /locus_tag="RH057_001027"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HDV3127436.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQIHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            complement(30445..32136)
                     /gene="fruA"
                     /locus_tag="RH057_001028"
     CDS             complement(30445..32136)
                     /gene="fruA"
                     /locus_tag="RH057_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HDV3127437.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTAQELDKAVAEATPYEPAGKTQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            complement(32153..33091)
                     /gene="fruK"
                     /locus_tag="RH057_001029"
     CDS             complement(32153..33091)
                     /gene="fruK"
                     /locus_tag="RH057_001029"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HDV3127438.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            complement(33091..34221)
                     /gene="fruB"
                     /locus_tag="RH057_001030"
     CDS             complement(33091..34221)
                     /gene="fruB"
                     /locus_tag="RH057_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HDV3127439.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDEHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEAGAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            34589..35770
                     /gene="setB"
                     /locus_tag="RH057_001031"
     CDS             34589..35770
                     /gene="setB"
                     /locus_tag="RH057_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HDV3127440.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTIEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPVILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFCLLRIKD
                     V"
     gene            complement(35767..36021)
                     /locus_tag="RH057_001032"
     CDS             complement(35767..36021)
                     /locus_tag="RH057_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV3127441.1"
                     /translation="MKCRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            36176..36748
                     /gene="yeiP"
                     /locus_tag="RH057_001033"
     CDS             36176..36748
                     /gene="yeiP"
                     /locus_tag="RH057_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HDV3127442.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLVKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            36971..38437
                     /locus_tag="RH057_001034"
     CDS             36971..38437
                     /locus_tag="RH057_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV3127443.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERGLTPFTVLSCDNIPDNGHVVKNAVLGMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFVAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAHISDCMQDRAFRHAARTLMLNE
                     QAPTLQIKDVDLTQYADKLIARFANPALKHKTWQIAMDGSQKLPQRMLAGIRIHLGCE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            38555..39541
                     /gene="yeiR"
                     /locus_tag="RH057_001035"
     CDS             38555..39541
                     /gene="yeiR"
                     /locus_tag="RH057_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HDV3127444.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            39580..40293
                     /gene="lpxT"
                     /locus_tag="RH057_001036"
     CDS             39580..40293
                     /gene="lpxT"
                     /locus_tag="RH057_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HDV3127445.1"
                     /translation="MIKNLPQIVLLNIVGLALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFVVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            40705..41271
                     /gene="mepS"
                     /locus_tag="RH057_001037"
     CDS             40705..41271
                     /gene="mepS"
                     /locus_tag="RH057_001037"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HDV3127446.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            41452..43008
                     /locus_tag="RH057_001038"
     CDS             41452..43008
                     /locus_tag="RH057_001038"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HDV3127447.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVRETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPGREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVAEGVETPEQARWLSERGVNFMQGYWISRPLPLDDFVR
                     WLKKPYTPQW"
     gene            43090..44904
                     /locus_tag="RH057_001039"
     CDS             43090..44904
                     /locus_tag="RH057_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3127448.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYAFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFMTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYRITSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRPVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPEAAELVLLAPMKKDLPSEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRSRDYDMMPRVWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            44905..45999
                     /locus_tag="RH057_001040"
     CDS             44905..45999
                     /locus_tag="RH057_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HDV3127449.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGIRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QQITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HVFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            45999..47024
                     /locus_tag="RH057_001041"
     CDS             45999..47024
                     /locus_tag="RH057_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3127450.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVIGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            47026..48615
                     /gene="yejF"
                     /locus_tag="RH057_001042"
     CDS             47026..48615
                     /gene="yejF"
                     /locus_tag="RH057_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HDV3127451.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHVSDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNYAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LINSQGSIIFDGQPLQNLNRRQLLPIRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(48619..48963)
                     /locus_tag="RH057_001043"
     CDS             complement(48619..48963)
                     /locus_tag="RH057_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127452.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
BASE COUNT        12031 a        12765 c        12083 g        12269 t
ORIGIN      
        1 atcccttacg gctaccgggt cgggcgcgag gtaacatccc tgtaaaacgc gccctcagcc
       61 cacatccatg tgggctgccc cggccttcag ggaacgcctc ggcaattttg acgccaccaa
      121 acaaccgtgc ggcctattga taaagagcta acacattgtc aaaaaacatc actatggttt
      181 tttagagttt ctcgatatca attgcctgaa tagcccttgc aatatcaggg gaattattca
      241 acacccgaac atgctgaaat aatcccgttg cttcatcgta ttctttacgc aaataactca
      301 accactgttt aatccgcgca acgtgatata acccggtatc gccctgcttt tccagacggg
      361 tatatttttg cagcaaagca accacctccg gccacggcat tcgcggttcg ttatatttta
      421 ctacccggct caggttggga atattgagcg ccccgcgacc aatcatcact gcgtcgcagc
      481 cgctgatcgc aatgcattgt tgcgcgctct gccagtccca gatttcgccg ttggcaatca
      541 ccggaatatt cagccgctgg cgaatctcgc caatcgcctg ccagtcaata tgctccgcgc
      601 ggtaaccctg ctcttttgtc cgtccatgca ccaccagctc cgtagcacca gcctgttgaa
      661 ccgcatcggc gatttcaaat ttcttctcac cgctgtccca gcccagacgc actttcacgc
      721 tgacgggcaa atgcgccggt acagcttcac gcatcgcttt tgcaccctgg tagatgagtt
      781 caggatcttt gagtaacgtc gccccaccac cgctaccgtt gaccgttttc gacgggcagc
      841 cgcaattgag atccacgccc caggaaccta attccaccgc acgggcggcg ttctctgcca
      901 gccattgtgg gaactgaccc aacaactgca cgcgcaccag cgtaccggat ggtgtccggc
      961 tggcgttttg tagctcaggg caaatgcgat gaaaaacttt taccggcagc agttgatcca
     1021 ccacgcggac aaactcggtg atgcacagat cgtagtcgtt aacttcggtc agcaattcac
     1081 gcaccagaga gtcaagcact ccctccatcg gtgccagtaa cacacgcata tcatcacccg
     1141 caaaataatg aggcgctatg ttagcgccta tggtcagcgg attaaaggtc tacaaagagg
     1201 gattgctgca aactgcaatc cctctctatg cttgatgtat gcctgagttc gttgcgcatt
     1261 gcgtaatgcc ggggagcgaa aaaataagcg ctgatttctt aatgtgatcg gtagcacgtt
     1321 ttacgaatta attgtatgat gaatccatct catctggggt gttgattatg agtaagacac
     1381 tgaacattat ctggcaatat ttacgcgctt tcgtcctgat ttatgcctgc ctgtatgcag
     1441 gcattttcat tgcttccctg ctaccggtaa ccattccggg cagcatcatc gggatgctga
     1501 tcctgtttgt cctgctggcc ttgcaaattc ttccggcgaa atgggtcaat ccggggtgct
     1561 acgtactgat tcgctatatg gcgctattgt ttgtgccgat tggcgtaggc gtcatgcaat
     1621 attttgattt gctccgcgcg cagtttggcc cggtagtggt ttcctgtgct gtcagtacgc
     1681 tggtggtttt tctggtggtg agctggagtt cacaactggt acacggtgaa cgtaaagtcg
     1741 taggtcagaa aggatcagaa gaatgatggc gaatatctgg tggtcattac cgctgacgtt
     1801 gattgtcttt tttgccgccc gcaaactggc ggcacggtat aagtttcccc tgcttaaccc
     1861 gttgctggta gcaatggtgg tgatcattcc ttttttaatg ctgaccggca tctcttacga
     1921 cagctacttt aaaggtagcg aagtgctaaa cgatctgctg caaccggcgg tcgtcgcgct
     1981 ggcctatcct ttatatgagc agctacacca gatccgcgcg cgctggaaat cgatcatcac
     2041 catctgtttt atcggcagcg tggttgcgat ggtgacgggg acttccgtgg cattgttgat
     2101 gggcgcttca ccggaaatcg ccgcgtcaat cctgccgaaa tcagtcacca cgcctattgc
     2161 aatggcggtt ggcggcagta ttggcggtat tccggcaatc agcgcagttt gcgtgatttt
     2221 cgtcggcatc ctcggcgcgg tatttggcca tacattgctt aatgcgatgc gtattcgtac
     2281 caaagctgcg cgcggtctgg caatggggac tgcctcgcac gccctcggta cggcgcgctg
     2341 cgccgagctg gattatcagg aaggtgcatt tagttcgcta gcgctggtgt tatgcgggat
     2401 aattacttcg ctgatcgcac cgttcctttt cccgattatt ctggcagtaa tgggctaaaa
     2461 tttgcgatgc gtcgcgcatt tttgatgtaa gtttcacgcg ttgcataatt aatgagattc
     2521 agatcacata taaagccata acgggttcgt aaactgttat cccattacat gattatgagg
     2581 caacgccatg catccacgtt ttcaaaccgc ttttgcccaa cttgcggata acttgcaatc
     2641 tgcactggaa ccaattctgg cagacaagta cttccccgct ttgttgaccg gggagcaagt
     2701 ctcatcgctg aagagcgcaa cgggactgga cgaagacgcg ctggcattcg cactacttcc
     2761 gctggcggcg gcctgtgcgc gtacgccatt gtcgaatttt aatgttggcg caattgcgcg
     2821 cggtgtgagc ggaacctggt atttcggtgc caatatggaa tttattggtg cgacaatgca
     2881 gcaaaccgtt catgccgaac aaagcgcgat cagccacgcc tggttgagtg gtgaaaaagc
     2941 gcttgcagcc atcaccgtta actacacgcc ttgtggtcac tgccgtcagt ttatgaatga
     3001 actgaacagc ggtctggatc tgcgtattca tttgccgggc cgcgaggcac acgcgctgcg
     3061 tgactatctg ccggatgcct ttgggccgaa agatctggag attaaaacgc tgctgatgga
     3121 cgagcaggat cacggctatg cgctgactgg tgatgcgctt tctcaggcgg cgattgcagc
     3181 ggcaaaccgt tcgcacatgc cttacagtaa gtcgccaagc ggtgtcgcgc tggaatgtaa
     3241 agacggtcgt attttcagcg gcagctacgc tgaaaacgcc gcattcaacc cgactctgcc
     3301 accgttgcag ggagcgttaa ttctattgaa tctcaagggt tatgattacc cggatatcca
     3361 gcgcgcggtt ctggcagaaa aagccgatgc gccgttgatt cagtgggatg ccacctccgc
     3421 aacgctgaaa gctctcggct gtcacagtat cgaccgcgta cttctcgctt aagcctggtg
     3481 ccggatgcgg cgtgaacgcc ttgtccggct tgccagccct ctcctggtgt cgaaattccc
     3541 ggcaaacagt ttgccgtttc ttgcgcaaaa ccagcgggta aagtagcctg atggaaattt
     3601 tccttagatc gagtctcctg catgttaaag cgcgtgttcc tcagcctgtt agtcctgatc
     3661 ggcttgctgc tgttgactgt gctcggcctc gatcgctgga tgagctggaa aaccgcgcct
     3721 tatatctacg acgaattgca ggatctcccc taccgccagg tcggcgtggt gctcggaaca
     3781 gcaaaatatt atcgtactgg cgtaattaat cagtattatc gctaccgcat tcaaggagcg
     3841 attaatgcct ataacagcgg taaggtaaat tatctattac tgagcggcga taacgcattg
     3901 caaagttata atgagccgat gaccatgcgc aaagatttaa tcgctgctgg tgtcgaccca
     3961 acagatattg ttctcgatta cgcaggcttt cgtacgctgg attccatcgt gcgtacacgc
     4021 aaagttttcg atactaatga tttcattatt atcacccaac gtttccactg tgagcgggca
     4081 ttatttattg cgctgcatat ggggattcag gctcagtgtt atgccgtacc gtcaccgaaa
     4141 gatatgctgt cagtacgtat tcgcgaattt gccgcccgtt tcggtgcgct ggctgacctt
     4201 tatattttta aacgtgaacc gcgtttttta gggccgctgg tccctattcc ggctatgcac
     4261 caggtgccgg aagatgcgca ggggtatccc gccgtcacac ccgaacagtt acttgaatta
     4321 caaaagaaac aaggaaagta gttatggatg tacagcagtt ttttgtcgtt gccgtttttt
     4381 tccttattcc gatattttgt ttccgcgaag catggaaagg ctggcgcgca ggcgcgattg
     4441 ataaacgggt taaaaatgca ccggaaccgg tgtatatctg gcgagcaaaa aatcccggac
     4501 tctttttcgc ttatatggtg gcatatatcg gcttcggaat tttatctatc ggcatgattg
     4561 tttatcttat tttctatcgt taatattttc tcttttctgt taattattga gaataattat
     4621 tacttcactc aataagctgc ggatataaca ttctgaaccg cagctatttg tgaatccttt
     4681 cacagtataa atttccccac gcgcatagcc ttaatatcag cacgcaatat ttatactcta
     4741 aataattcga gttgcaggaa ggcgacaagt gagtgagtcc ccaggagctt acataagtaa
     4801 gtgactgggg tgagcgaacg cagacgcagc acatgcaact tgaagtatga cgagtatatt
     4861 tagcgttcgc cttaattact catagcatta aggaagatca catgccgcaa caaaattatc
     4921 tggatgaact cactccggct tttacgcctt tactggcgat taaagaagcc tctcgctgtt
     4981 tattatgtca tgacgctccc tgtagtcagg cttgcccggc acagaccgat ccggggaaat
     5041 ttattcgctc aatctacttt cgtaatttta aaggcgctgc cgagacaatt cgcgaaaata
     5101 atgccctcgg tgccgtttgt gccagagtgt gcccgacgga gaaattatgt caaagcggtt
     5161 gtacccgtgc cggtgttgat actcccattg atatcggtcg cttacagcgt tttgttactg
     5221 attttgaaca acaaactggg atggaaattt atcagcccgg tactaaaacg ctcggcaaag
     5281 tcgcgattat tggcgcaggt cctgccggat tgcaggccag tgtaacactg acaaaccagg
     5341 gttatgacgt cacgatttat gagaaagaag cgcaccccgg tggctggttg cgtaacggta
     5401 ttccgcaatt ccggttaccg caatcagtgc tggatgcaga gatcgcccgt attgaaaaaa
     5461 tgggcgtgac cattaagtgc aacaacgaag tcggtaacac actcaccctt gagcagctga
     5521 aagcagaaaa ccgcgcggta ctggtcaccg tggggttatc aagcggttcc gggctaccgc
     5581 tgtttgagca tagtgacgtt gagattgccg tcgacttcct gcaacgtgta cgacaggcgc
     5641 aaggcgatat cagcattcca caaagcgcat taattatcgg cggcggtgat gtcgcgatgg
     5701 acgtagccag cacgctgaaa gttctcggct gtcaggcggt aacctgcgta gcgcgtgaag
     5761 agttagatga gtttccggca agcgaaaaag agtttgccag cgcccgggag ctgggcgttt
     5821 cgatcatcga tggattcacg ccagtagccg tggaaggcaa taaagtcacg tttaagcatg
     5881 tacggttacc gggcgaactg acgatggcgg cagataaaat tattctcgcc gtcggtcagc
     5941 atgccagact ggatgccttt gcggagttag agccgcagcg taacaccatc aaaacacaaa
     6001 attatcagac ccgcgatccg caagtctttg ctgctggcga tattgttgag ggtgacaaaa
     6061 ccgtggtcta tgccgtgaaa accgggaaag aagccgccga ggcgattcat cactatttag
     6121 agggagcttg ctcatgttaa cgaaagatct ttcgattact ttttgcggcg tgaagtttcc
     6181 caacccgttc tgcctctctt cttcgcctgt aggcaactgc tatgagatgt gtgccaaagc
     6241 ctacgacaca ggttggggcg gcgtggtgtt taaaacgatc ggctttttta tcgccaacga
     6301 agtctcgccg cgttttgatc atctggtgaa agaagatacc ggttttatcg gcttcaaaaa
     6361 tatggagcag attgctgaac atccgttgga agagaatctg gccgccctgc gtcagctgaa
     6421 ggaagattac ccggacaaag tattgatcgc ttcgatcatg ggggaaaatg agcagcaatg
     6481 ggaggagctg gcgcgcctgg tgcaagaagc tggcgcggat atgatcgagt gtaacttctc
     6541 ctgtccgcaa atgacttctc atgcgatggg tagcgatgtc gggcaaagcc cggagctggt
     6601 agaaaaatat tgtcgggcag tgaaacgggg ttccacgctg ccaatgctgg cgaagatgac
     6661 gccgaatatc ggtgatatgt gcgaagtggc gctggcggcg aagcgcggcg gcgcagatgg
     6721 cattgcggcg attaacaccg ttaaatccat caccaatatc gatcttaatc agaaaatcgg
     6781 tatgccgatc gttaacggaa aatcgagtat ttccggatat tccggtaaag cggtaaaacc
     6841 gatcgccctg cgcttcattc agcagatgcg cacccatcca gaactgtgcg atttcccaat
     6901 cagcggtatc ggcggcattg aaacctggga ggatgcggct gagtttttat tgctcggcgc
     6961 agcaacgtta caggtgacca ccggcatcat gcagtacggg tatcggatag tggaagatat
     7021 ggcaagcggg ttgtcgcatt atctcgccga tcagggattt gattcgctgc aggagatggt
     7081 aggtctggcg aataacaata ttgtcccggc ggaagattta gaccgcagtt atattgtcta
     7141 tccccgtatc aatcttgata aatgtgttgg ctgtggacgc tgttatattt cctgttacga
     7201 cggcggtcac caggcgatgg aatggagcga gaaaacccgc acaccgcatt gtaataccga
     7261 gaaatgtgtg ggttgtctgc tttgtggtca cgtctgcccg gtgggttgta ttgagctcgg
     7321 ggaagtgaag tttaagaaag gcgagaaaga acacccggta acgttgtaaa accagagacg
     7381 catccggcat ttggcaccga ttgccggatg cagcgcatcc ggcaatatcc acactcacat
     7441 cacttcttac gcgcgtattt cagtgaatcc agcgctacgg cgaagataat aatcgcccct
     7501 ttgatgatgt actgccagta tgggtttacg ccgatatacg tcaggccata gttgatgacg
     7561 gtaaaaataa ttaccccggt caccacgcca atcaccgtcc ccacaccgcc gctgaacgat
     7621 acaccgccta ccacgcacgc cgcgatagca tccagctcat acataaagcc gaggttgttg
     7681 gtggcagagc cgatacgtcc ggcttctaac atcccgccaa aggcatagaa cacgccagac
     7741 aacgcgtaga tcatcagcag gttcaggccg acgttgacac cagatacttt tgccgcttcc
     7801 gggttaccgc caatggcaaa aatgttctta ccgaagcggg ttttgttcca caacacccag
     7861 acgaacgcca ccgcaatcaa cgcgtagaag gtgatgtaag agagacggaa actccccagc
     7921 gcgacaaagc cctgagcaaa ggtagagaag ccactgtcaa aaccagaaat tggcgacgcc
     7981 ccgacaaagt cgtaatagag cgagttgatg ccatagacga tgatcatcgt gccgagcgtg
     8041 gtaatgaacg gcgtcacgtt gagataagcg ataatcagac cgttgatcaa accgatcacc
     8101 gcaccaatgg cacagacaat cagaataacc agcgcaatcg gcatcgtcgc catttccggg
     8161 aacactttgt tggcgttatc catggactgc aataatgtcg cagccaccac tgccgccagc
     8221 cctacctgac gaccagcaga aagatcggtc ccctgggtga caattaaccc tgccacaccg
     8281 agcgcgataa taatacgcac cgatgactgg gtgagaatat tacttaagtt caacagactt
     8341 aaaaatgttg ggtcctggaa aataataatc gccagcaaaa ctaaaagaac gacgtaaata
     8401 ccgccctctt tcaggtaagt aagaaaactt ttcttattta acgcactcat gggaagcccc
     8461 taatcttaaa ggtgcaaaga cgcaagacgc agaatttcgt tttgcgttgt tgtttttgta
     8521 tcgacaattc cggaaacgag accattgctc atgaccagaa tacggtctgt tatccctaac
     8581 aactcaggca tttcagagga gataataata atccccttgc ctttcttcgc cagttcggca
     8641 attaactgat aaatttcaaa cttcgccccg acatcaatac cgcgcgtcgg ttcatcgagc
     8701 attaatattt ctggttgcgt tagtagccag cgaccaataa tcaccttttg ctgattacca
     8761 ccggagagcg aaccaatttg cgtccgatga cccggcgttt ttacccgcat cgaatcaatc
     8821 acccactggg tatcgctttt catccgcgag ttatccagta aaccaacttt atttttgtag
     8881 ttgcgaatat tggaaattaa cgagttaaaa ccaatatcca gataggcata aattcccgtt
     8941 gagcggcgct cctcagttac cagtgcaaat ccgtggttta tggcttcgtt ggcattatgg
     9001 ttattgatct gtttgccgtg caacgtaatg gtgccagccg atttctcgcg aataccaaat
     9061 aacgtctcaa caatatcggt acgtttcgcc cccaccagcc cggcaatacc gaggatctcc
     9121 cctttatgca gatcaaacga gacatcgcga atcgacggct ggcgcagtga cgttaggtta
     9181 cgcacctcga ggatgacttc gcccggcttg ttttctttgt caggaaaacg ctgattaaga
     9241 gaacgcccaa ccatcatggc gatgatcttg tccatcgtca gtcctgccag cggttcggtg
     9301 gcgatccact gaccgtcgcg caatacggta acttcatcac ataactggaa gatttcttcc
     9361 attttatgcg agatataaac aataccgcag ccgcgctctt ttaatttacg aataatagtg
     9421 aacagatgat tgacctcttt ttcggttaac gaagaagtcg gttcatccat aatcacaatt
     9481 ttcgcgttat aggaaaacgc tttggcgatt tcgatcatct gcatttgcga aacggataat
     9541 gtgccgacgc gcgcacgcgg atcgatatca atatccagtt catcaaaaat cgctttggtt
     9601 tcgcggtaca ttttgtcctg atcgacaaac atgcctttgg tgggatatcg ccccagccac
     9661 atgttatcca tcaccgaacg ttgtaatacc aggtttaact cctggtgtac catcgaaata
     9721 ccattttcca gtgcttcttt ggcagaatgg aaatcgatct ctttaccctg gaataaaatg
     9781 gtgccggagt ctttttgata aataccaaac aggcatttta ataatgtcga ttttcctgca
     9841 ccgttttccc ccattaatgc atggatagaa tgtggccgga cttttaaatt aacgttatca
     9901 agtgccttaa caccaggaaa ggacttgttg ataccgctca tttccaacaa gtattccccg
     9961 gaagacggag tcgttgagct gaccatataa ttttaccttg ttggccatac aataagggcg
    10021 cagtaaacga ctgcgcccaa tcagtcttat ttcttgctga attcagccag gttgtcttta
    10081 tctacgccaa cataaggtac gcggaccact ttgttgtcga ttttccagtt ggtgccatca
    10141 gccgcacctt tgccatcggc caggtttttc gccagatcaa aggtcgcttt cgcctggttg
    10201 ttagcatcgt tcagtactgt gcccgccagt gcaccggatt tcaccagcgc cagcgcttct
    10261 ggcagcgcat cgacgccaaa caccggaatg ctggacttgt tgtgtgcttt cagtgcttct
    10321 accgcgccca ttgccatcgc atcgttgttg gcgataacca cttcgatttt gttggcgttc
    10381 gggccagaca gccaggcgtc catcttatct ttcgcctgag cggtatccca catagcggta
    10441 tctaactgta actgttcagt tttaatgcct ttgtcgttca gctctttaat cacgtaagtg
    10501 gtacgtgctt ctgcatccgg atggcccggt tcacctttca gcaatacgaa ttgaatctga
    10561 ccgtctttgt tcagatccca accctgattc gccgcccagt gtttagcaat caaatcgccc
    10621 tgaataatgc cggactcttt ggaatcagtg ccaacgtagt aggctttgtc gtagctatcc
    10681 agcgccttac gagacggttc tttgttgaag aaaaccaccg gcacgttttg cccacgcgct
    10741 ttctcaatca ccgtacccgc agctgccggg tcaaccaggt tgattgccag tgccttcacc
    10801 cctttcgcca gcaatacgtc gatctgatcg ttctgcttgg actggtcatt ctgagaatca
    10861 ttcatcagca gctgaacatc tggcgcggct ttcgcatctt gctcaatagc cttgcgcact
    10921 acagacataa agttatcgtc gtacttatag attgttacac caatgcgagt atcagcagcg
    10981 tgtgcagcgg caccgaataa catgctggcc atcacagcag acagggttaa caccttctta
    11041 ttcatggtat ctccggtttt tcttatgcag ggtagtgctt gagataaatg ctcggcgggg
    11101 cagtagagtt aatgaagtgt tactgaacgc cgaagctcac tttttaaaat tcgttcttcc
    11161 atgcccggta acgctccaga aaacggcttt aattgttgtt atgacgctat tacctcggtg
    11221 aaagtgatta ttcactgtta catacgggtt acaacgttaa aacggtgcaa tcatagctat
    11281 cacattgtta agatactgtg aaatcactca cagattgaaa gcggttacat cgcctgattt
    11341 gttgagttag tgatcgtcgc cgcattctgg cgtgttgcga cagaatggcg acgcactaac
    11401 gtcggcataa aacagtggct ggcacgagga tcaatattgc ctgctgcccc ctgcaaggcc
    11461 agttcggtgg ctaatttcgc cattgaagca atgggataac gcacggtcgt taattgcggg
    11521 tcggtgtaac gggcgatggg aatatcatcg aaaccgatga ttgagagatg taacggaatc
    11581 gcaatgccat tatcttttaa tgctgtcagt gcgccagcgg ccatattgtc gttataagca
    11641 aatacagcgg taagttgtag attgcgcccc agcagttcaa ccatcgccgc ctcaccgccc
    11701 ggcatgtccg gcgtaccagt gccaatccag ctttccggcg gaataatatc ctgctctttc
    11761 aacgcactca tccagcctgc tttacgcatg gcgtcatctt caatgccgtg gctggaagaa
    11821 agataaccaa tacgttgatg accgttattc agcaacatgc gcgtcgccat tcgggcaccg
    11881 ctgagattat ccaggcaaac gcaacgatgg gcgtaccccg gcacaacgcg gttgattaac
    11941 accataccgg gaatgttatc cataaattgc gccagttcat cgtcactcaa tgcttttgag
    12001 tgaacaatca acgcattaca acgctggcga attaacacct caatggcgtg acgctctttt
    12061 tccgcttcat gatagctatt gccgattagc acgtatttct gatgctgctg ggcgaccaga
    12121 tccaccgctt ttaccagcgc gccgaaaaac gcatcagaaa cgtccatcac caccacgcca
    12181 atggtatcgc taacctgagt tgccagcgcc tgggcattgg catttggccg ataatccagc
    12241 tcactcacgg ctttcattac tgcttcacgc gtgtcggcac tgaccagcgt gctgttattg
    12301 agcacccggg aaaccgttgc cacagagacg ccagcctgac gcgctacatc acgaatggtg
    12361 atcatattca ctacctgtta tgagattgca gtaaatgact gcttgctggc ggctattttg
    12421 tcagcactga atacaggact tcgtgaatcg agtcacagca atggaaacgg ttacagccgt
    12481 tttgttaacg aatgtgatcc agatcgttat cttgatgttt tagatattgc cgttccggca
    12541 gcacgcaaag ttaactgacg ccagagccat tccaccggcc cctggcggaa gtaacgcagc
    12601 cagataacag agaagaggat attcgccagc cataccggaa taacaaacgc cagcagctcc
    12661 aggcgatcaa aatgcataaa caaaccgagg tggtaaaaaa gcgtggtaca aatcagcgtt
    12721 tgcaatagat agttggtcag cgccatccgt ccgacgcagg cgattgccag caccagctta
    12781 aagcggctta attgcggcca gaagccataa aacagcgacg catagccaat cgcctgaaac
    12841 ggcgcactca gttcccgcgg catttgaagt aagaaggcac accagcgata cgcccagtcc
    12901 agctgccatt gcagggcgat ggcaggaagg ttaatggtca ccccaatcgc caccagcaca
    12961 aaaccagtac gacgatagtg acgtaagctg aactgccctt tcagccagcc gctgcgcatc
    13021 agcgccgcac caatgagcat catcccagcc agttgccagc catactgtgc gcccagtgcc
    13081 agtaaactgt tgccaacacc atcggcacga ttactgatcg catcaacgcc gccgtgaagc
    13141 ttccagtatt tttcatacaa aatagccgat gcatccggcg tccaggcgcg gctggtctgg
    13201 ctgtcggaaa tcagccccaa taacagcaaa acgccaagcc ccaccagata aagcatgacg
    13261 cctgtattaa acaggctttt taccgatggc gcatcgcgca ccagccgcca gcagattaag
    13321 cccaccagcc cgtaagccag cagaatatcg ccgtcccaga acaataaacc gtgaataaag
    13381 cccaataaga ctaacagcgt taaccgcgac tggatccagc gtctgccacg gggcagcaac
    13441 atttgcagtc cagcaccaaa cagcagcgca aaaagcgtga ggaatttcac ctggccgatg
    13501 agatcaagaa atgcccaggt ccatgcatcc tgcggcgtaa tagcaccgta ccaggcggga
    13561 ttgagataag ccgcctttgg tagcccaaag gcgctgatgt ttaatagcag gatccccaga
    13621 atggcgacgc cgcgaacaaa atcgagcgtg acgttgcgct ccatggttcc tgccttttaa
    13681 tcagttgtga tgacgcacag cgcgcagaaa ctcgtggcgc gtattctgac tggatttgaa
    13741 caatccacca agagaggtcg ttgttgtggc actggttgca tcgcggatgc cacgcgcctt
    13801 cacgcagtaa tgcaccgcgt cgatcgagac agccacgtta ttggtgccca gcagcgtttg
    13861 tagcgcaata agaatttgct gcgtcagacg ttcctgcacc tgcggacgct gggcaaagaa
    13921 ctgcacgata cggttaattt ttgacagacc gatcaccgaa tctttcggga tataagccac
    13981 cgtcgctttg ccatcgatgg taacaaaatg gtgttcacag gtactggtca gagtgatatc
    14041 gcgcacggtg accatttcat cgaccttcat tttgttttca atgagggtga ttttcgggaa
    14101 gttggcgtaa tccagaccgg agaaaatttc atcgacatac attttagcga tgcgatgcgg
    14161 cgtttccatc aaactgtcat cagccaggtc gagattcagc agctgcatga tttcggtcat
    14221 atgaccagca ataaggcttt tgcgcgtttc gttatccatt tcatgcacgg gcgggcgcag
    14281 cggtgtttcc agtcctcgcg caactaacgc ttcatgaacc agggccgctt ctttactgag
    14341 tgatggcatt tatgatttct cctgcaggtg tgacgcctcc gccctgcgtg ggggcaaagt
    14401 tattaagttg atttacagcc tgattattgt gcgtgaggcg gcgcacataa tccagtattc
    14461 acagcgataa ttattgtaat tgccgctgcc tttcatcagc agatgttaaa acatcgttat
    14521 gcaaatacgg aagtgaaagt tactcacagc acattgaata aacggtatga tgaagaaatt
    14581 gcaaacaaca caacaaggag ccacgcatgg aaatgctcga agagcaccgc tgttttgaag
    14641 gctggcagca acgctggcga cacgactcca gtaccttaaa ctgcccgatg acgttcagta
    14701 tctttctccc accccctcgt gatcacaccc cgccaccagt gctgtactgg ctttccggat
    14761 taacctgcaa tgacgagaac ttcaccacca aggcgggtgc ccagcgcgtg gcggcagaac
    14821 tggggattgt actggtgatg ccagacacca gcccgcgcgg cgaaaaggtt gccaacgacg
    14881 atggctacga tttaggccag ggcgcaggct tttatcttaa tgccacgcaa ccgccgtggg
    14941 cgacgcatta ccggatgtat gattatctgc gcgatgaatt accggcgctg gttcagtcgc
    15001 aatttaatgt cagcgaccgc tgcgccatta gcggtcactc aatgggtggt cacggtgcgc
    15061 tgattatggc gctgaaaaat ccgggtaaat acaccagcgt ttcggccttt gcgccaattg
    15121 tgaatccgtg cagcgtcccg tggggaatca aagcgtttag cagctattta ggtgaggaca
    15181 aaaatgcatg gctggaatgg gacagttgcg cgctgatgta tgccagtaac gcgcaggatg
    15241 cgatcccgac gcttatcgat cagggcgata atgatcagtt tcttgccgac cagttgcaac
    15301 ctgcggtact ggcagaagcc gcgcgccaga aagcgtggcc gatgacgctg cgtattcagc
    15361 cgggatatga tcacagttac tacttcatcg cctcttttat agaggatcac ctgcgcttcc
    15421 atgcgcagta tttactgaag tgaaagtccg cccggttcgc cgggcatctt ctcatcagaa
    15481 gcgataatcc actgccataa agtaacgacg tccgtcttcg ttatagctgt agtcgtcacg
    15541 actgagatcc ttgtcgccaa ggttcagcac gcctgcgcgc agtttgacgt ctttagtcac
    15601 ctgccaggcc gcgccggtat tccagatggt ataaccgccc ggtgttttag ccgtcgcgct
    15661 gtcggcgcgt ttctgcccgg tatagtgacc agaaacatag aatgaccagt cttccagcgc
    15721 cagcggtttc cagtccagcg taccgttagc agtatggaac ggcagatcgg atagcggttt
    15781 gttttcgccg ttgctgacat cacgaccatc gttgtaggtg tagttgatcg acagtttcca
    15841 ttcatcgttg aacggaattt tcagttcggt ttccacgccc tgaatacgag ctttgttaac
    15901 gttgtagtag ctaaataccg gtatgcgccg tccgttagcg cccgtctcaa aaccaacaaa
    15961 gttttggtag cccggtgcag cgttgacgtc agacgtacgg ctgatgctga tacgatcttt
    16021 cacatcgtta cggaaaacgg taacgctgga ttcaacccct tccagccagc cttcttcacc
    16081 catgtagtaa agccccagct cccaactttc gctggtttct ggtttcagat ccgggctacc
    16141 cacaatctta catgcgccac ggcaggaatt gctcgtccag tcagggctaa gttgcaacag
    16201 agaaggtgct ttaaatgccg tcgcccagcc ccctttcacc gttacggtgt cggtggcgtt
    16261 ataaaccagg taggcacgcg gactccagtg ttcaccgtag gtttcgtgat cgtccatacg
    16321 cacgccggtc gtcagcgcca gcggctcgaa gatccgccat tcatcttcca caaacagcgc
    16381 gtactggctg gcagacgttt tggagctggt tcccccggtc aggttcaccg catcgctaag
    16441 tttgtcgtga cgccattcac cgccaaccgt gagaaactga ttaatcgccg tcagcggcaa
    16501 cgtgtatttg ccgtcgaccg tattgctttc ggaagttatc gggctgctgt tgccagggtt
    16561 tttgttctcg actttctcac cgtagtattt cagttcgctg gtgccgtaat cccaacgccc
    16621 attatggctg acggagtagt tctggcgttc caggcggttt ttgtccagcg agtcggaatc
    16681 acgatcctga cggtcgaaac cgtatccggc agtaaaatcg tgattttgat tcggtgtcca
    16741 ggcaaattcg acattgccgt cgcggctgga gaatccttca atacgcggcg tttctccggt
    16801 atcggtggtc gttgagtttt gcgggtcatc cttttcacgt tttgccaggc tgccgtaagc
    16861 tttcattccc agcacaccat caattaatgg tccactggta aagaactgac cgttataggt
    16921 atcaccgcga tcgcgatgtt cctgaatggt ggtatcgacg gtaacggtac ccgaccattt
    16981 ctgaccgatt tttttggtga tgatattcac tacaccgccg agcgcatcgg agccgtatag
    17041 cgacgacatc gggccacgaa ccacttcaat acgttcgatg gaatcgaccg ggatccagtt
    17101 cagatcgaaa tcattgtggc ggaagacggc attgcgggag ttaacgcgtt taccgtcgac
    17161 gagaatcagg gtgtagctgc tgtccagacc acgaatacta acgcccttac ggttatcccc
    17221 tgttaacgcg tttaccgtcg acgagaatca gggtgtagct gctgtccaga ccacgaatac
    17281 taacgccctt acggttatcc ccttcgttcg tcagttgtac gccaggcact tctttgagga
    17341 catccttcag attctgtacc ggttttcgct gcaggtcttc ctgggtaatg acgctgatac
    17401 tggcgggtgc atctttgagg ttttgttcaa cggaagatgc agtgacaacc atcgtttcgc
    17461 catcatcatc gaccgctaac acaggccatg cacaagaaat agcggacaaa cacagcccga
    17521 cccgtacgaa agggttcaac ctaaacattc catatctcca tgaggtaact acgaaaataa
    17581 aatgggttat cgctcacatc ttcttcacgt ccccttgcgt acggcagcat cgcggtggac
    17641 ttatttgtat gcaggtgatc atcccagaaa gccggaacag cctctgattt gtcagccttt
    17701 tttgattgcg gcgtaacgat aatgcaaacg ataacaatta tcaatccaaa tgttaaattt
    17761 tatatccgca tgatttgtca gggaaatgga attgaaaaag ccctctcggt tgagagggct
    17821 tagcaaggaa gggaggaaac ttatttctta tcgttctgcg ggaacttcat ttcgctgtaa
    17881 cgtacgaagt gagttccttt aatcagcttg tagccgaacc aaataatcag gaacagcggg
    17941 ataccaatat acgttgccgc tacgccgccc cagtcaatgg tatctttcag gaacgcttcg
    18001 tagttctggc ccaaagtgat aatcagacac agaatgaatg cgaagatcgg ccccagtggg
    18061 aagaaacctg aacggtacgg cagatcgtta atgtcgtgtc cctgcaatac gtaaccgcga
    18121 cggaagcgat agtggctaat ggcaatcccc agccaggcga taaaacccgt catcccggag
    18181 gtgttcagca gccacaggta taccgtctgg ttgccaaaca tggaggtcag gaagcacaga
    18241 ccggcaatca ccgtcgtcgc atacagcgca ttacgcggca cgccaccacg cgacagttta
    18301 gcgaaaatgc gcggcgcttt accgtcacac gccagggtgt acagcatacg agtagacgca
    18361 tacataccgg agttacccgc tgacagcacc gccgtcagaa taactgcgtt catcaccgcc
    18421 gccgcagaga gcagacccgc gtgctggaac accagggtga acggactaac gctgatgtct
    18481 ttaacatcgt tacgcagcag gctcggatcg gtgtacggaa taatcaggct gataatcagg
    18541 atcgcgaaca catagaacaa caggattcgc cagaacacct gacgtaccgc gcgtggaatg
    18601 tttttcgccg gatcttcgga ctcgcctgca gcaataccga tcagctcggt tccctggaaa
    18661 gagaagccga caatcatagc tacgccgatc atcgccgcaa aaccaccagc aaacggcgct
    18721 tcgccgattg tccagttgct ccagcccgca ggctgcgcgc ctttgaagat accgataatc
    18781 atcagcacgc caacgatgat aaagacaata actgtcgtga ctttgatcag tgagaaccag
    18841 tattccgctt caccaaagcc acgaactgag atgtagttca gcaggaagat aacgccgagg
    18901 aacaacgcac tccagatcca gcccggtgta tccgggaacc accagctcat gaccagctga
    18961 gctgcaacca ggtcaacggc gatagtcacc gcccagttgt accagtagtt ccagcccagc
    19021 gcgaagccaa agccttcttc aacatagttc tgaccgtaag tggcaaacga accggaaacc
    19081 ggcatatatg cagccagttc accgagactg gtcatcagga agtaaaccat caggccaatc
    19141 agcatatacg agagcaatgc cccgcccgga cctgcctgag aaatcgttgc gccagaggca
    19201 acaaaaagac ctgtaccgat ggaaccgcca atggcaatca tcgtcaggtg acgcgccttt
    19261 aattcacggc gtaagcccgg cgcttctgtg gttttagttt cggaaaccat acaaaaatgc
    19321 tatccatctt aaaaatgagg cgcgattgta gcagacgatt ggcaatcctt ccggcagaaa
    19381 tacacggtta taagacacct tcatgatcgc ccagggatta taagtaaagc agccaatctc
    19441 ttttctggcg aatgcatgct gagggcgctg cgtcacgaaa tcgacacagc accagcatgt
    19501 tcttgtacag caacttaacg cggtacattc gcgggatcgc aatagtccag aaagcgccgt
    19561 agcgcgttgg aaaggtgttt ttgccgatga tgtatacgcc acaacgtacg catcaggcgc
    19621 ggcagaggga ccgcaacttc acttaatgtg cctgcctgca attgatcttc aatcacccgt
    19681 cgcgacaggc aactaattcc caacccatga cgcaccgcat gtttgattgc ctcggagtta
    19741 cctaattcca tcgccatctc aaacttcggt aaatgtgaca gcaatagata atcgacaatc
    19801 tcccgcgtgc cggaaccgcg ttcacgcagg atccacggcg cagcggccag ctgctctaag
    19861 gtgaccggac cacgggccaa cggcgaagtc ggcgcggcga aaaccaccag ctcgtcttcc
    19921 agccacggtt cagaaataat ttcagtgctg tggcacggtc cttcaataaa accaatatca
    19981 acgcggaaat ccagcaccgc ttgcatcacg tcctggctgt tcccaacgct aagttcaatc
    20041 ggcaactgcg gataatcatg gcgataacgg gcgataactg caggcagaat gtagttaccg
    20101 atggtactac tggcatagat acgaatcgcg ccgttgtctt cgcgaaacag ttgttcgatt
    20161 tcaaccgcct gttcaagcaa tgccaacgca cgcggataga gcagccgccc gtgttcatta
    20221 acaaccagtc ttttccccac gcgatcaaac agttgcacgc caagctgccc ttccaggtcg
    20281 gtcaaggctg cgctcactgc tgattgcgac aacgccagca tcaccgacgc ctgggtggtt
    20341 gatccacttt tcaatacttc tgcaaaaact tccaactgcc ggagggtgat gtgcatagtc
    20401 gcttaccact tataaagatt aattataaat atataatcaa ttttattttt aaaccagtta
    20461 gtcgtaacct tatgcccggt taaaggagag ggttatgacg aatatcacct tacagaaaca
    20521 acatcgtaca ctgtggcatt ttattccggg gttagccctg agtgcagtta tcaccggggt
    20581 cgccctgtgg ggtggttcca ttcccgcggt tgcgggtgcc gggtttagtg ccctcaccct
    20641 cgcaatcttg ttggggatgg ttttaggcaa caccatctat ccgcacatct ggaaaagctg
    20701 tgacggtggt gtgctgtttg ccaagcaata tttattacgt ctgggtatca ttctttatgg
    20761 cttccgtctg acgttctcgc aaattgccga tgtcggtatc agtgggatca tcattgacgt
    20821 gttgacgctg tccagtacct tcctgctggc ttgcttcctg gggcagaaag tgtttggtct
    20881 ggataagcac accagctggt tgatcggtgc cggtagcagt atctgtggtg ctgccgcggt
    20941 actggcgact gagccggtag tgaaagcgga agccagtaaa gtaaccgtgg ctgttgcaac
    21001 cgttgttatc ttcgggaccg tcgcgatttt cctctacccg gcgatatatc cgctgatgtc
    21061 tcaatggttt agtccggaaa ctttcggtat ctacattggt tctactgtgc acgaagtggc
    21121 gcaggtggtg gcggcaggtc atgccatcag cccggatgcg gaaaacgcag cagttatttc
    21181 caaaatgctg cgcgtgatga tgctggctcc gttcctcatc ctgctggcgg cgcgtgttaa
    21241 acagctgtct ggggcgaaca gcggcgagaa aagcaaaatc actattccgt ggtttgccat
    21301 cttgttcatc gtagttgcca tctttaactc gttccacctg ttaccgcaga gcgtggtgaa
    21361 catgctggta acgctggata ccttcctgct ggcaatggcg atggcggcgc ttggtctgac
    21421 cactcacgtc agcgcgctga aaaaagccgg ggcgaaacct ctgctgatgg cactggtact
    21481 gtttgcctgg ctgattgttg gtggtggtgc gattaactat gtgattcaaa gcgtcatcgc
    21541 ataaaccact acatcttgct cctgttaacc cgctatcatt accgttttcc tccagcgggt
    21601 ttaacaggag tcctcgcatg aaatacattg gagcgcacgt tagtgctgct ggcggtctgg
    21661 caaatgccgc aattcgcgcc gccgaaatcg acgcaaccgc gtttgccttg ttcaccaaaa
    21721 accaacgtca gtggcgtgcc gcaccgctca cgacgcaaac catcgatgaa ttcaaagcgg
    21781 cctgtgaaaa atatcactac acatcggcgc aaattcttcc ccacgacagc tatctgatta
    21841 acctcggaca tccggtcact gaagctctgg aaaaatcgcg cgatgccttt atagatgaaa
    21901 tgcagcgttg cgaacaactg gggctttctt tgctcaactt ccaccctggc agccatctga
    21961 tgcagatttc agaagaggat tgccttgcgc gtattgccga atccatcaac attgcgctgg
    22021 ataaaactca aggtgtaaca gcggtgattg aaaacaccgc cggtcagggc agtaacttag
    22081 ggtttaaatt cgaacatctc gcggcgatta tcgacggcgt ggaagataaa tcccgtgtcg
    22141 gcgtctgcat tgatacctgc catgctttcg ctgccgggta tgatttgcgt actccagccg
    22201 aatgcgaaaa aacgttcgct gattttgccc gtattgtcgg ctttaagtat ctgcgcggga
    22261 tgcaccttaa cgatgcgaaa agtacctttg gcagccgcgt tgaccgccat catagcctcg
    22321 gtgaaggcaa tatcggtcat gatgcgttcc gctggatcat gcaagacgac cgtttcgacg
    22381 gcattccgct gatccttgaa accatcaacc cggatatctg ggcagaagag atcgcctggc
    22441 tgaaagcgca acaaactgaa aaagcggtgg cctgatcgat gaacgacagg gaaaagcaga
    22501 ttcttaaaat tttacggcgt aacccgctga ttcaacagaa tgaaatcgcc gatattctgc
    22561 aaatcagccg ctcacgtgtt gccgctcata ttatggattt gatgcgcaag ggtttgatta
    22621 aaggcaaagg atatatcctt accgagcagg attactgtgt ggttgtgggt gcaatcaata
    22681 tggatattcg cggaatggcg gatatccgtt acccgcaagc agcctctcat ccaggttctg
    22741 ttcactgttc cgcaggcggc gtcggacgta atatcgcgca taacctggct ctgcttggtc
    22801 gggatgtgca tttaatatca gctattggca acgattttta cggcgagaca ttactggaag
    22861 aaacgcgccg cgcgggcgtg aacgtttcaa actgcattcg tctgcatgga cacagcactg
    22921 caacttatct cgctatcgct aataagcagg aagaaactat cctggcaatc aatgacacac
    22981 atattctgca acagctaacg cctcaactgc tcaatacttc acgggacttg attcgccatg
    23041 ctggtgtggt gctggctgat tgtaatctca cacctgaagc cctggagtgg gtctttacca
    23101 ttgctgatga aattccgatg tttgtcgata ccgtttccga atttaaagcg aacaaagtaa
    23161 aaaactggta cagccggatc cacactctga aaccaacaca aaatgaactg gaaattttgt
    23221 gggggcaacc gattaaagac gacaacgatc gcatcagagc tgtaaattcg ctgcatcaac
    23281 aaggtgttaa acgcattttt gtttatctga aagatgaatc tgtattttgc agcgataagg
    23341 atggagaaca atttttgctg accgcgccag cgcatacgac agtagacagt tttggtgctg
    23401 acgatggttt tatggcgggc ttggtatata gctttcttga aggatacagt ttccgcgaca
    23461 gcgcccgttt tgcggtagcc tgcgcggcaa tttcgcgcgc cagcggcagc ttaaacaacc
    23521 ctaccctgtc tgccgataac gcgctttcat tagtgccaat ggtgtaacaa tgttgccgga
    23581 ttcgacgctt gaccgcatct tatccggctc ataaattgcg cactacgcta aaccaataaa
    23641 gaatcctgca atagtagcac tcatcaggtt agaaagtgtc gccgccgcca gcgcgcgtaa
    23701 accaagttgg gcgatttccg gcgcacggtg tggcgcaacc gcagaaaacg cccccaccac
    23761 caccccgata gaaccaaagt tagcgaaacc gcacaacgcg aaagaaataa tcgccacggt
    23821 tttagcatcc agagtgccac ccgtttgcag atatggcgag aaattgagat aagcgacaaa
    23881 ttcattgatc gccagcttct gcccaatcaa actcccggca agatttgcat cactccagtc
    23941 aacccccatc acccacgcca atggggccaa caggtaacct aaaatggact ccagcgaggc
    24001 atgtgcaaaa ccaaaccagc cgccaacgcc gccgataata ccattaatta acgcaatgat
    24061 ggcgacaaat gccataacaa ctgtcgctac accggcggcg attttcagcc cggtcattgc
    24121 ccctgtcgca gcggcttcaa taatgctttt tggcggtgtt tcggtgaaag agaggttatt
    24181 aaaggaaacc tgcgaagatt ccgtagccgg gcttaacagg cgggcaaaca agatcccgcc
    24241 tgggatcgcc attaacgatg ccgccagcaa atattccaca ggtacgccca gggcggcgta
    24301 accaatcatt gtcgaaccag caatcgaggc catgccacta caaatcgctg taaataattc
    24361 attgcgattc agacgatcga taaagggttt gacgattgcc ggaatttcgt tttgcccgag
    24421 gaaaatggta gtgaccgcga cgaatgactc gatcttgctg atatttaacg ctttctggaa
    24481 gataccgccg agaatgcgaa ttaaaatccc catcacaccg atgtagtaga gaatactcac
    24541 cagtgctgta acgaagataa ttgccggtaa tactctgaaa ccaaagataa atcctgcgcc
    24601 atcaaacagc gtgtccattt tcggcccgac cagcgaaccg aagataaatg cgctacccgc
    24661 gtcgctgtac gccatcactt tatgcacgcc aaaagcgact ttttcagcga cccaacgccc
    24721 tggcggtaac caaagcataa tgccgccaat cacaacctgt aacactaacg ccgcgccaac
    24781 ggtacgcagg ctgatcttct tcttgtttac tgacagcaaa aatgcaattg ccagcaatac
    24841 caccattccc agaacacttc tcatgacatc catagtgatt atctcttcat gtcatattgg
    24901 gcgacggctt tctgcccatc agaaatagcc gggcctgcat cttgcgctat cggcccggct
    24961 tgttatcaac gcttaatggg ttttgatgta gccgcgcacg cactcttcga ctaagcccca
    25021 gaaccagtcg gtatcaatag tgatgccgac tttggtattg gcgggcttgc caagaacgcc
    25081 cagctcgtcg cagacggtgc gcccatagca agggccactg ttgacgtcca cttcgacgta
    25141 catctcctgg gttttaatgc catcagggtt aatcagataa ccgatgcagg tggcgtcgtg
    25201 caccgggccg ccagccaggc cgtagttttc gaactgcgtt ttgagagtga agttcatgat
    25261 gtcgctgaac agctctccgg cagggccgcc tgccctttcc atccgagcaa tcacgtccgg
    25321 ggtgcaaacg gtctggttgg tgagatcgag gcccatcatc actaatggaa cgccagaggt
    25381 gaacaccacg cgggctgctt ccgggtcagc aaagatgttg aactcggcag atggcgtgaa
    25441 gttgcctgta ccgtaagcgc cgcccattag cacaatttca cggattttgg gcaggatcgc
    25501 gggttgcata cgcattgcca ccgcgatatt tgaaagcgga ccaaccggca ccagagtgat
    25561 atcgccatcg cttgccatca gggtatcaat gatgtatttc accgcgtggg tactttctgc
    25621 ctggcgggtc agcggctcga ataccgggcc atccagtccg gtttcaccgt gaatattatc
    25681 ggcaacgatt tgttgacgca taatgggttg cggcatcccc gcataaaccg gaacattaat
    25741 ctccagtttc tggcaaacat tcaggccatt aattaatgtt ttatcaagcg tctgattacc
    25801 cgctacaata gtgatgccta ataaatctat tgccggatgt ttcgctgcca tcatcattgc
    25861 aatagcatca tcatgacccg gatcacaatc cagaataatt tttctctttt ccattgttta
    25921 tttcctctgt ttccagttgc gttatttttt ctacagcaaa gaaaattcga ccgggccgat
    25981 gattgaatct taccaaccgc gtacgtatgc taaatatgag aaatctcata gcggataaac
    26041 atcgtgaaag aaatccacaa taatgatctt aagcagcaat tgatgagtga atccgcgttt
    26101 aaggattgct ttttaacgga tgtttcagcc gatacgcggc tgtttcattt tttagcgcgt
    26161 gactacattg tgcaggaagg gcaacagccg tcctggctgt tttacctgac gcgaggccgc
    26221 gccaggcttt acgccacgct agctaatggt cgcgtgtcgc tgatcgattt ctttgccgcc
    26281 ccctgtttta ttggcgagat tgagttaatc gacaaagacc atgaaccgcg tgcggtgcag
    26341 gctattgaag agtgttggtg ccttgcgctc cctatgaaac attaccgccc gcggttatta
    26401 aacgacacgc tatttttacg aaaactctgc gtcaccttaa gtcataaaaa ttatcgtaat
    26461 attgtttctt taactcagaa tcaatcattt ccgttagtga atcgcctggc agcatttata
    26521 ttactctcgc aggaaggtga tctttatcac gaaaagcata cgcaagcggc agagtattta
    26581 ggcgtttctt atcgacatct tttatatgtt ctcgcgcaat tcattcacga cggtttatta
    26641 attaaaagca agaaagggta tctaattaaa aacagaaagc agttgtcagg actggcgctg
    26701 gagatggacc cggagaataa attctccggg atgatgcagt aaaaattatt tgcaatagcg
    26761 cgattgccgg atgcaacact taataagcgc gtacatccgg caatgaaact caagctaaac
    26821 caataaagaa cccggcaatg gtcgcactca tcaggttgga aagcgttgct gctgccagcg
    26881 cccgtaaacc aagctgggcg atttccggcg cgcgttttgg cgaaatagcc gaaaatgcgc
    26941 caacgacaac accgatagaa ccaaagttag caaaaccaca aagcgcaaag gagataatcg
    27001 caatggtttt cacttccagc gtgccgcccg tttgcaggta cggggagaaa ctcaggtaag
    27061 cgacgaattc gttaatcgcc agtttctgcc caatcaggct acccacaaga ttggcatcac
    27121 tccagtccac acccatgatc cacgccagcg gtgccagcac atagccaaaa atactttcca
    27181 gagaggcatt ggcgaaacca aaccagccac caattccgcc gataatgccg ttgatcagcg
    27241 caataattgc gacaaacgcc attaccaccg tcgctacacc agcggcgatt ttcagcccgg
    27301 tcatcgcacc gctcgccgcc gcttcgataa agctttttgg cggcgtttcg ctgaacgaca
    27361 gattttcaaa tgtgacctgt gaaggctcgg tggccgggct aagaatacgt gcaaacaaaa
    27421 taccacctgg gatcgccatc agcgatgccg ctaacaggta gtcaattggt acgcccattc
    27481 cggcataacc aatcatcatc gaaccagcaa tggacgccat cccgctacaa attgcggtaa
    27541 acaactcgtt gcgattcatg cgatcgataa acggtttaac gatcgccggg atctcatttt
    27601 gcccgaggaa aatagttgtg actgcgacaa aagattcgat tttgctgatg ttaagagctt
    27661 tctggaaaat gctgccaagg atgcgaatca gcagccccat cacgccaatg tagtacagca
    27721 gactgatgag cgcagtaacg aaaataatcg ccggaagtac gcgaaaggcg aagataaaac
    27781 ccgcaccgtc aaacaagaca tccattttcg gcccaaccag cgaaccaaaa atgaaggcgc
    27841 taccggcatc actgtaagac atcactttat gaacgcctaa tgccgcctgt tctactgccc
    27901 actttcccgg tgggaagtag agcatgatgc caccaatagc gatttgcagc agcagtgcgg
    27961 ctccaaccgt gcgcaaactg atgctctttt tattcactga caacagaaat gctattgcca
    28021 gtaacaccac catccccaca acacttctca ttatatccat aatgattttc ccttcatgcc
    28081 agtaaacccg gcgtcagcgc caggttttgg tatgcttgat gagtacgggc gacggctttc
    28141 tgcccgtcag aaattaaccc gcgagacgct ggtattcttt ggcaatttca ctcgccagaa
    28201 tggcgttgtt gaacaccagc tggatattgg atttcaggct gtcaccgccg gtcagctcag
    28261 caacgcgcgc cagcaggaac ggtgtacttt ctttaccaat aacgccctgc tcttcggctt
    28321 cggcgacggc ttgatctatg gctgcattga tagattcctc cggcatcgca aactgttccg
    28381 ggatcgggtt cgctaccacg aggccaccgt tcagcccgct ttgccatttc accgccattg
    28441 cacgggcaat ttcactggcg ctgtcgagac gaatgctgac gtcaaacgag ctggtacggc
    28501 agaaaaacgc aggcagcgct ttagtctgat agccaattaa cggcacaccg aaggtttcta
    28561 aatactcagt ggttaatcct aaatcgagaa tagatttcgc cccggcacaa acaacggtga
    28621 tattagtatt tgccagttct tgcaaatcgg cagaaatatc gaaggtatgt tccgccccgc
    28681 gatgcacacc accaattccc ccggtggcaa atactttaat tccggcaagc gccgcaataa
    28741 tcatcgttga agccacagtg gttgcgccat tttttccggc ggcaacaaca aaaggtaaat
    28801 cgcgacgact aactttggtc acgttatgcc cttcacgacc cagtaattca atttcttctt
    28861 tgcttaaacc cactttcatc acgccgccaa taatggcgat agtggcaggt actgcgccct
    28921 gtttacgaat agtttcttct acttcaattg cggtctgggc attttgtggg aacggcatcc
    28981 cgtgagaaat aatggtcgat tccagcgcca caaccggttt tttgtttttt aaagcgtcct
    29041 gcacttccgg ggaaatttgt aataattcag gggaaatttt taattcagac attctgcgtt
    29101 ctccactaac gatataacgt tggcaatcga taaatcgggg ttattggtgt attcacagga
    29161 gagcgccatt gacgaacatc cctgtgcgaa acgaacagat tcggcaaacg gcattccgtc
    29221 tacccaacac gaagcaagtc ccgccatcat ggcatcgccc gctccggtaa cattaataac
    29281 attggttttg atcggcgcag accagccatt ttcaccgctg atatcgctgt aataaacgcc
    29341 gtcgccgccc atgctcaata ccagtcggtt caggccatgt tgatggaacc aggcagcaac
    29401 ttttgccaca tcgtcacgcc ctgacagcgc aatcccactc agggtttccg cttcaaggcg
    29461 gtttggcttg agagtgtgga tctgatttag acggtcgcgc actttgacac atttccacgc
    29521 tgaaaccgga tcgacaaata cgggtacgtt ggcggcatta tccagaatcc atgccagtgc
    29581 ctcttcactg atattacagt ctgcgacaat gacctttgcc ctctgaataa attcacggtg
    29641 ctgtgcgaga tattcagctg taatagcgtt gctaatattc atgtcattta tagcaaccag
    29701 catttcaccg gtattatcga gtaatgataa atagcttgac gtattttctc ctggcacaat
    29761 caggcattta tcgacataaa cgccagattg attggtttgc gttagcagcg attgaccata
    29821 aaagtcactg cctacggcgc tcagtagcca ggctttgttc cccagcaacg ccaggttttg
    29881 tgcaatattg cgcccgactc caccaggcgt aaattttatt ttacctggat ttgaatccgc
    29941 ataatttaat gattcatgtg aatatccggc gacatcaata ttcgccgagc ctataattac
    30001 gacataatcc ttttcgcgca tagcacgtcc tctggtaaaa aattaccaac cctgtcaatc
    30061 cacaagaata tagatactgc aaagacagtt taaacatgtg ttcatattta aacatgtgct
    30121 catagtatgc atgttttacg taaagtaaat tgccgccaga gaggcaaagc gggcagattt
    30181 gagcaggctc acaaaatgcg attaaatgcc agaattacaa aagcgaggaa tttgtgacgg
    30241 gatgcacaga acaggaacac taaaaaaggg cagaaaagaa tctgcccttg aggaaataac
    30301 cttagtgttt gatgaacgtt tcaggtttct actgaaagat aaaaccattt aaattcataa
    30361 aattacattc acaaaccggt cattctcttt gcgctcatcc aatgatacag ggcagtaaat
    30421 ttctgccctg taacacacct tttattacgc ggctttcgct actgcgtcca cttccggacg
    30481 tttcaggaag gcataggcca aacccgccac cagcgtaccg gcaataattg ctaccaggta
    30541 acccaatacc ggcgtaatag cgccagggat cagcagaaca aacagaccac cgtgcggtgc
    30601 catcagtttc gcaccaatcg ccattgagat tgcgccagtc agcgccccac ccacgataca
    30661 gcacggcagc acacgcatcg gatcacgagc agcaaacgga attgcacctt ccgaaataaa
    30721 gcacagtccc agtaccagag cggctttgcc accttcctgc tgcgctttgt cgaatttgcg
    30781 acgcgccacc attgttgcca ggcccattgc cagcggtggc accatacctg ccgccataat
    30841 cgccgccatc gggccatagg tttgagtact cagcagaccc acaccgaatg cgtacgctgc
    30901 tttatttacc ggaccgccca tgtcagtaca catcatgcca ccgaggatcg cccccagcag
    30961 aaccgcattc gcagtcccca tggtctgcag ccagtgagtc aacccttcga gaatgccagc
    31021 aaccggttta ccgatcaggt agatcatcgc cagaccgacc accagactgg aaattagcgg
    31081 aatgatcagg atcggtttca gcgcctccat actctgtggc agtttcagtt gcgtactgat
    31141 taacttcgca atgtaaccag ccaggaagcc cgcaataata ccgccaatga agccagaacc
    31201 ggtgctgacc gccagcatac cgccaatcag acccggcgtg aggcccggac gatcggcaat
    31261 ggaaaaggca atataacctg ccagtaccgg taccatcagc gcaaaggctg aaccaccacc
    31321 aatctgcatc agcgccgctg ccaacgtacc cggctcttta aacgcttcga taccaaaagc
    31381 aaaagaaagc gcgatacaca gaccaccagc aacgaccatc ggcagcatgt aagagacgcc
    31441 cgttagcaag tgacgataag cgcctgcact ctctttctta ccttcagtgg tcgccgtttg
    31501 agttttgccc gccggttcat acggcgttgc ttcagcaacc gctttatcca gttcctgcgc
    31561 ggttttcttc agcgccagac cggtagaggt acgatacatc ggtttaccag caaatttcgc
    31621 cagatccact tcgatatctg ccgccacaat caccagatcc gctgcggcta cttcctcggg
    31681 agtgattgca ttacccgcgc caacagaacc acgggtttca actttcaccc accagccacg
    31741 ttttttcgct tcggtttcaa tggcttcagc cgccataaag gtgtgtgcta cgccagtcgg
    31801 gcaagcagtc accgcaacta cgcgtttcgg accgctggcg gcaaccggtg ctgtcgcagt
    31861 gaccggcgca gtgtaaggtt tcgcatggcc tttggcttca ctcaggaaca gctcagggtg
    31921 cgcaactgcc cgggaaatat cacccagcca gacattttta ccgttcagcg cgctgtcatt
    31981 cgggatggaa tcaccgagaa caatcgccat ttctgcgtcg ttcggattgt cgatgatttc
    32041 cagttttgct tttcgcgccg ccgcgcccag cagggtcttc gccatatagg cgcgtgcctg
    32101 accgagatta gcgtcaataa tcagcagcgt tttcattatg cctctcctgc tgtcagttaa
    32161 aaggttgtaa gtcgacgcgc gccatcattg cggccaactg cggacgatcg gtaataccca
    32221 cattgctttg acttaccgcc agggctgcaa cagcagtcgc cagacgcagt gtgtgttcac
    32281 tggattcacg catcagcaag ccataaatca ggccaccaac catagaatcc cctgcgccaa
    32341 cggtgcttac gacatcgact gacggtggtt tggcgatcca ttcgccggag gcattaaccc
    32401 aaagcgcgcc ttcggcaccc agtgaaataa caacatgcgc aataccttgt tcacgtagcg
    32461 catgtgcagc ttcaatcaca tctttcattt caggcagttt acggcctgcc cagatttcca
    32521 actcgcggcg gttaggtttc accagccacg gtgccgcttt caaacctgct actaacgctt
    32581 cacggctact atcaaagata atgcaaggac actgactacg caggcgagtc atccagtcgg
    32641 tgaacgcttc cgggctgacg cctgacggta agcttccgct gacacagacc atatcgaact
    32701 gaccgagcca gctcagagaa tcagtcacaa agcgttccca gtcggcgggg gtgacttcaa
    32761 aacccgagaa gttgaagtcg gtcacttcgc cgtctttttc cgtcagctta acgttaattc
    32821 gggtgcgccc ctgtacaacc tggaaacggt tggcaatgcc cagctcgctg aacagttgct
    32881 gaaaaccatc ctgattgtct ttacccagga agccgccaac ggtgacatca attcccaggt
    32941 cttttaatac tttggccacg ttgatgcctt tacccgccgc atgcagaccg gtggttttca
    33001 ccaggttcac ttcgccgcgt tcaatttccg ggcagaaacc aacaaggtca taagccggat
    33061 taagggtgat agtagcaaca cgtctgctca ttatgcgccc tccccaagac cagcagcgat
    33121 agcgtcgccg attgctttca gcgcctgttc agcatctgca ccctgggcgg taaagcgtag
    33181 gcgatgacct ttcttaacgc caagtgccac aactttcatc agactacgtc cgtttgccgg
    33241 tttgccggta ccatcaaggt ttgtcacggt aatatcactg ttaaattgtt taatggtatt
    33301 gaccagcatg gtacctggac gagcatgcag gccgtgttca ttgcgcacca caaactccgc
    33361 gcttaacacg tcgtcggtcg gcgcatcatc gctggtcagc agcgccagca acgttgccgc
    33421 atccgctttc agcaagcggt cagctttatt gtcgagcaat aaatcagcga gacgcttaag
    33481 aaccgcgatg ggctgatcgt cattcatcgc cacactcacc agcatggctg ccgtttcgcc
    33541 gtccacatca aaagcatttg ccgcacggct taccgcaatc gcgctacgca gattgccttc
    33601 ggcgctatcg ctcagccaga taccctgtcc gagattcagc ggttgttcat tgatggcttt
    33661 ggtgacgaaa gtggcgtcaa ctgcccccgc ctctttcaga cgcgcagcgt tcagcgcctg
    33721 aagagtcagc agatcgctgg cgacgatatc cagcgtcagc atttcgttgt cgagcttcag
    33781 ctgctcactc tgcttttcgc ccatcagtaa tgcgcgaagt tcttctgctg ttgttgctga
    33841 cttcagttgt tcagcaacgg aatcatcgct cagtacgtgg gtcagctggc gtagcaggcc
    33901 cagatgttca tccgagctgg cagcaatacc gattgccacg tacgctacct gaccgtcacc
    33961 ccaggtgacg ccttccggga actgaaatac ctgaacgccg gttttcagca cctgatcgcg
    34021 ggtgtcggta gtgccgtgtg gaatagcaat accattgccg aggaacgttg aggtttgctg
    34081 ttcgcgcgcc agcatgccat tgacgtagcc ttctgctaca ttaccggcct gcaccagcgc
    34141 cgcagcgacc tggcgaatcg cctcttcttt gtctccggcc ttttcgcccg gatggatgtc
    34201 ctgtacggat aactggaaca tagttctcct ctcttgctga attgaaacga ttcagcctct
    34261 atgagaaaaa aagcgccaac ctggcttagg gttaaagaca agatcgcgct gaaacgtttc
    34321 aagaaagcat aatacttctg tttcagcacg caaggaaagc cgtaaagtta tgagcaaaag
    34381 tttgatgtgc tgcacaattt tttgccattt ttcctaattg ctgatgggaa aatccgttat
    34441 cagcaatttc atttcagcag cgtcagcagc caatcagcaa cagtgaaatg gcattttgat
    34501 tattcgtggc gcttttgatt tcgctgtctg tttattttct gacaagcagc gtaaactccg
    34561 cgtcttcctc ttccagtgat cgaccagcat gcataactcc cccgcagtct ccagcgcgaa
    34621 atcgtttgac ctgacctcga cggcgttttt aatcgttgcc tttctcaccg gtattgcggg
    34681 cgctctgcaa accccgacac tcagtatttt tcttactgat gaagtacatg ctcgtccggc
    34741 gatggtggga ttcttcttta ccggcagcgc tgtcattggg attctggtaa gtcagtttct
    34801 cgccgggcgc tctgataagc gcggcgatcg caaatcgctg attgtctttt gctgcctgtt
    34861 aggcgtgctg gcctgcaccc tttttgcctg gaatcgcaac tactttgttt tgctattcgt
    34921 tggcgtcttt cttagcagct ttggctcgac cgctaacccg caaatgtttg cccttgcccg
    34981 tgaacatgcc gacaaaaccg gacgtgaggc ggtgatgttc agctcttttt tacgcgctca
    35041 ggtttcactg gcatgggtca ttggcccacc gctggcttat gccttagcga tgggtttcag
    35101 ctttacggta atgtatctga gcgcagcggt agcgtttatt gtttgcggtg tgatggtgtg
    35161 gctgttttta ccgtcgatgc aaaaagagct tccgctggcg accggcacga tcgaagcgcc
    35221 gcgccgtaac cgtcgcgata cgctgctgct gtttgtcatt tgtacattga tgtggggctc
    35281 gaacagcctg tacatcatca acatgccgct atttattatc aacgaactgc atcttcccga
    35341 gaaactggcc ggtgtgatga tggggaccgc cgccgggctg gaaatcccga cgatgttgat
    35401 tgccggatat ttcgccaaac gtctgggtaa gcgtttctta atgcgcgttg ctgccgtggg
    35461 tggcgtctgt ttttacgcag gaatgctgat ggcgcattca cctgtcattc tgttgggctt
    35521 gcagctgcta aatgctattt ttattggcat tctgggcggc atcgggatgc tctattttca
    35581 ggatctgatg cccggtcagg cgggttcagc caccacgctc tataccaaca cgtcgcgcgt
    35641 gggctggatc atcgcaggat cagtggcggg catcgtcgcc gagatctgga attatcacgc
    35701 tgtgttctgg tttgcgatgg tgatgattat cgccactctg ttttgcttac tgcggattaa
    35761 agatgtttaa ggcgcggtca gcatttccag atcgataagc caggtcatgg cctgctgacg
    35821 cgagttaccg cacatttcag cgctggcttg taagcctgca cagactttcg gtcgcagtgg
    35881 cgaggtgaaa attttacagc gttgctgttc atcaagctga atgcagggcg tattggcggg
    35941 cttgccatct ggcataccgg gaatagggct ggaaattgat ggggcagtgc aacaagcccc
    36001 gcaacccgga cggcatttca tacgttgttc tcttaaggtt aaccaccgga ttgatgagcg
    36061 cgcacagtac cacctttttt gcaccagcaa aagtgcgaat accacttgcc cgaaaggccc
    36121 gtcgcgagta ctttgtcgcg atatttttga cattttcgac tacaggaatt tttcgatgcc
    36181 aagagcgaac gaaattaaaa aaggtatggt actgaattac aacggcaaac tgctgctggt
    36241 taaggatatt gatattcagt cgcccactgc ccgcggtgcc gctacgctgt acaaaatgcg
    36301 tttttctgat gtccgtaccg ggctgaaagt agaagagcgt ttcaaaggtg atgatatcgt
    36361 tgacaccgtg acgctgaccc gccgttacgt tgacttctcc tatgtcgatg gcaacgaata
    36421 tgtctttatg gataaagaag actatacccc gtataccttc accaaagatc agattgaaga
    36481 agagttgctg tttatgccag agggcggcat gccggacatg caggtgctga cctgggacgg
    36541 tcaactgctg gcgcttgagc tgccgcagac cgttgatctg gaaatcgttg aaaccgcacc
    36601 aggcatcaaa ggggcatctg ccagcgcccg taacaaaccg gcgacattga gcactggtct
    36661 ggtgattcag gtaccagaat acttaagccc gggcgaaaaa attcgtatcc atatcgaaga
    36721 acgccgttat atggggcgtg ctgactaact tcagccgcat gcagaaaagg gatagctcag
    36781 gctgtccctt ttttaattta ttataccaat cttctatttt gcgcttcctg aacaccttac
    36841 gccctcaatt ttcactcgtt gattgatcgc cctcacactt catcgcatta acaatccaga
    36901 ccaatttcaa ttgctgtcat ataactttac actgtcattg ttaatttatc gttactaaga
    36961 cgtgactcct atgaagacaa ttgcctccgt tacgctcccg catcatgtac acgctccacg
    37021 ctacgatcgc cagcagttgc aatcacgtat cgttcatttt ggctttggcg cctttcaccg
    37081 cgctcatcag gcgttactga ccgatcgtgt gctgaatgcc cagggcggcg actgggggat
    37141 ctgtgaaatc agcttgttca gcggtgatca actgatgagc cagctccgcg cacagaacca
    37201 tttatatacc gtgctggaaa aaggtgcgga cggcaatcag gtgataattg tcggtgccgt
    37261 tcacgaatgc cttaatgcaa aactggattc cttagcggca attattgaga aattttgcga
    37321 gccacaggtg gcaattgttt ccctgacgat taccgaaaaa ggctattgta ttgacccggc
    37381 caccggtgca ctcgacacca gtaatccgcg gattattcac gatctacaaa cccctgaaga
    37441 acctcactcc gcaccgggta ttctcgtcga agcactgaaa cgccgccgtg agcgcggcct
    37501 tacaccgttt accgtgctct cctgcgacaa tattcccgac aatggccatg tggtgaaaaa
    37561 cgcggtgctg ggaatggcag aaaaacgttc gccagaactc gccgggtgga taaaagaaca
    37621 cgtcagtttt ccgggaacca tggtcgaccg cattgttccg gctgcaaccg acgaatcact
    37681 ggcagaaatc agccagcatc tgggggtaaa tgatccctgc gcgattagct gcgaaccgtt
    37741 tatccagtgg gtggtggaag ataacttcgt cgctgggcgt cctgcctggg aagtcgcagg
    37801 tgtacaaatg gtgaatgatg tcctgccatg ggaagagatg aaactgcgta tgcttaatgg
    37861 cagccactct tttctcgctt atctgggtta cctttcagga ttcgcccata tcagtgattg
    37921 catgcaggat cgcgcatttc gccatgccgc cagaacatta atgctgaatg agcaagcgcc
    37981 gacactacaa attaaagatg tcgatttaac acaatatgcg gacaagttaa ttgcacgttt
    38041 tgctaatccg gcgctgaaac ataagacctg gcaaatcgca atggatggta gccagaaatt
    38101 accgcaacgt atgctggcag gtattcgcat acatctgggg tgcgaaacgg actggtcgtt
    38161 gctggcatta ggcgttgctg gctggatgcg ttacgtcagc ggcgttgatg atgccggaaa
    38221 tgccattgat gttcgcgatc cgcttagcga taaaattcgc gaacttgttg cgggcagcag
    38281 cagtgaacaa cgcgtaaccg ccctgctttc cctgcgtgaa gttttcggtg atgatctgcc
    38341 agataacccg cattttgtgc aggccatcga acaagcctgg caacaaatcg tacaattcgg
    38401 cgcacatcag gcgctattaa acaccctcaa aatttaacga tttctgcggt taaagcggat
    38461 gaagctcacc ttcgtccgct ctccccttct cttttctgcc ttttttagcc aggattaacg
    38521 ctcagttaac ttaccagagt attgcggagc cgttgtgacc aggaccaacc tcatcaccgg
    38581 ttttctcggc agcgggaaaa ccacgtcgat tcttcatctg ttagcccata aagatcccaa
    38641 cgaaaaatgg gcggtactgg ttaatgaatt tggggaagtc ggaattgatg gtgctttgct
    38701 cgccgatagc ggcgcattgc tgaaagagat ccccggcggc tgcatgtgct gcgttaatgg
    38761 tttacccatg caggtagggt tgaatacctt actgcgtcag ggaaaaccag accgcttgtt
    38821 gatagagccg accgggctgg gccatccgaa acagatcctc gatctgttaa ccgcaccagt
    38881 ctatgaaccg tggatagatc tgcgcgccac cttgtgcatt ctcgatccac gcctgctgct
    38941 ggacgaaaaa agcgccagca atgaaaactt ccgtgaccag ctggctgccg cagacatcat
    39001 tgtcgccaat aaatccgacc gtgcgacgcc cgaaagtgag caagcgctac agcgttggtg
    39061 gcagcaaaat ggtggcgatc gacaattaat tcacagtgag catgggaaag ttgacggtca
    39121 tcttctggat ttgccgcgtc gcaatttagc cgagttgccc gccagcgccg cgcattctca
    39181 tcagcatgtc gtgaaaaaag ggttagcagc gttaagcctg ccagagcatc aacgctggcg
    39241 tcgcagtctg aacagcgggc aaggatatca ggcctgcggc tggatattcg acgctgatac
    39301 ggtattcgac accattggca ttctggaatg ggcgcgactt gcaccggtgg aacgcgtcaa
    39361 aggcgtgctg cgtattcccg aagggctggt acgaatcaac cgtcagggcg atgacctgca
    39421 cattgaaacg caaaacgttg cgccaccgga cagccgtatt gagctgattt ccagcagcga
    39481 agctgactgg aatgctttgc agagcgcgct gttgaagctt cgtttagcga ctaccgcgta
    39541 aggttgcctg cgtttttcag taagataatt agagaaaata tgattaaaaa tttgccgcaa
    39601 atagtgttgt tgaatattgt cggcctcgcg ctgtttcttt cctggtatat tcccgttaat
    39661 catggattct ggttgccaat tgatgcggat attttttatt tctttaatca gaaactggtc
    39721 gaaagtaagg cctttttgtg gctggttgca ttgaccaaca atcgcgcttt cgacggttgt
    39781 tctctgctgg cgatgggaat gttgatgctg agtttctggc tgaaagaaaa cgcccctggc
    39841 agacgacgta tcgtgattat gggtctggtc atgctattaa ctgcagtggt attaaaccag
    39901 ctgggtcagg cattaattcc tgtaaaacgg gccagcccaa cattgacttt taccgatatt
    39961 aaccgcgtca gcgaactgct ctctgttccc acgaaagatg cctcacgaga tagctttccc
    40021 ggcgatcacg gcatgatgct gcttattttt tcggcattca tgtggcgtta tttcggcaaa
    40081 gttgcaggcc ttatcgccct tattattttt gtggtttttg catttcccag agtaatgatt
    40141 ggcgcacact ggtttactga catcattgtc ggttcgatga ccgtgatatt gatcggtttg
    40201 ccctgggtgt tgctgacgcc attaagtgat cgattaatca ccttttttga caaatcacta
    40261 ccgggaaaaa acaaacattt ccaaaacaaa taactcacag taattaacat catcaggctt
    40321 atttttatag tgaggataat cctgatgatg cgcaccgtgc tttcatccat cgcgcgcaaa
    40381 aatcattctc taagtaaatg aatggattgc atgcgtttca ctcaatcgta ctttaattga
    40441 ccaatcccgc ttattaactt tctgtatcac tttttcttat aaaaaatcat gtaaaaccgc
    40501 tcgccaagac cgcaccaatc gggtaatctc gaactcgttt tgcctcggcg gtagattatc
    40561 ctcacagcat ataattttgt gcgttagtcc acagatttgg ccttaaggaa ttgtttcaac
    40621 atgcccaggt aattagtctc gtgtcgcttg gcattttttt ataacgatat ttgtcgttaa
    40681 ggacttcaag ggaaaacaaa caacatggtc aaatctcaac cgattttgag atatatcttg
    40741 cgcgggattc ccgcgattgc agtagcggtt ctgctttctg catgtagtgc aaataacacc
    40801 gcaaagaata tgcatcctga gacacgtgca gtgggtagtg aaacatcatc actgcaagct
    40861 tctcaggatg aatttgaaaa cctggttcgt aatgtcgacg taaaatcgcg aattatggat
    40921 cagtatgctg actggaaagg cgtacgttat cgtctgggcg gcagcactaa aaaaggtatc
    40981 gattgttctg gtttcgtaca gcgtacattc cgtgagcaat ttggcttaga acttccgcgt
    41041 tcgacttacg aacagcagga aatgggtaaa tctgtttccc gcagtaattt gcgtacgggt
    41101 gatttagttc tgttccgtgc cggttcaacg ggacgccatg tcggtattta tatcggcaac
    41161 aatcagtttg tccatgcttc caccagcagt ggtgttatta tttccagcat gaatgaaccg
    41221 tactggaaga agcgttacaa cgaagcacgc cgggttctca gccgcagcta ataaaccgtt
    41281 tggatgcaat cccttggcta tcctgacgag ttaactgaaa gcactgctta ggcagtgctt
    41341 ttttgttttc attcatcaga gaaaatgatg tttccgcgtc ttgatccagg ctatagtccg
    41401 gtcattgtta tcttttaaat gttgtcgtaa tttcaggaaa ttaacggaat catgttcata
    41461 cgcgctccca attctggacg taagctcctg cttacctgca ttgttgcagg cgtgatgatt
    41521 gcgatactgg tgagttgcct tcaattttta gtggcctggc ataagcacga agtcaaatac
    41581 gacacactga ttatcgacgt acaaaagtat ctcgatacct attttgccga cctgaaatcc
    41641 actactgacc ggcttcagcc gctgacctta gatacctgcc agcaggctaa ccccgaactg
    41701 accgctcgcg cggcgtttag catgaatgtc cgtacgtttg tgctggtgaa agataaaaaa
    41761 acattctgtt catctgcgac tggtgagatg gacattccac tcaatgaatt gattccggcg
    41821 ctcgacatta ataaaaacgt cgatatggcg atcttacccg gcacgccgat ggtgccgaac
    41881 aaacccgcaa tcgtcatctg gtatcgcaac cctttgctga aaaatagcgg tgtgtttgcc
    41941 gctctgaatc tcaacctgac gccttctctc ttttatagtt cacggcagga agattacgat
    42001 ggcgtcgccc tcattattgg caatactgcg ctatctacct tttcttcacg tttgatgaac
    42061 gttaatgaat taaccgacat gccggtccgt gaaactaaaa ttgcgggcat tcctctgacc
    42121 gttcggcttt atgcggatga ctggacatgg aacgatgtgt ggtacgcatt tttactgggc
    42181 ggcatgagtg gaactgtcgt tggcctgctc tgctattacc tgatgagcgt acgtatgcgc
    42241 cccggcagag aaatcatgac cgccatcaag cgcgaacaat tttacgtggc gtatcaaccg
    42301 gtggtggata cacaagcttt gcgagtaacg ggcctggaag tactgctacg ctggcggcat
    42361 cctgtcgcgg gagaaattcc cccggatgcc ttcattaact ttgccgaatc gcaaaagatg
    42421 attgtgccgc tgactcagca cctgtttgag ttaattgccc gcgatgccgc agaattagaa
    42481 aaagtgctgc cggtaggcgt caaatttggc attaacattg cgccggacca tctgcacagc
    42541 gaaagcttta aagcagatat ccagaaactg ctcacttccc tacccgcaca ccatttccag
    42601 attgtgctgg aaattaccga gcgcgatatg ctgaaagagc aagaagccac acaactcttc
    42661 gcctggctgc actcggtcgg cgtagaaatt gctattgatg acttcggcac cgggcacagc
    42721 gcgcttatct atcttgagcg ttttacgctc gattatctga aaattgaccg tggatttatc
    42781 aacgccatcg gtacggaaac gatcacttca cccgtacttg acgcggtgct gacgctggcg
    42841 aaacgcctca atatgctgac ggttgctgaa ggggtcgaaa cgccggaaca ggcgcgatgg
    42901 ctaagcgaac gcggcgttaa tttcatgcaa ggctactgga ttagtcgccc gttaccgctg
    42961 gacgattttg ttcgctggct aaagaaaccg tatacgccgc agtggtaagg tgtgcttacg
    43021 tcccttatta ttcatagtga aagcatgccg gattgcggct aatgatgagt aaaaggaaat
    43081 ccgttgcaga tgattgtgcg catactgctg ctgtttatcg ctctgttcac ctttggtgcg
    43141 caggcgcagg ctatcaagga aagctatgcc tttgccgtgc tgggcgaacc ccggtacgcg
    43201 tttaatttca accattttga ttatgtgaac cccgccgcgc caaaaggtgg gcagataacg
    43261 ttgtcagccc tcggtacctt cgataatttc aaccgctatg cactgcgcgg caacccgggc
    43321 gcacgcaccg agcagctgta cgacacgcta tttacgactt ccgatgacga accaggcagt
    43381 tattacccgc tgattgctga aagcgcacgc tatgctgacg attattcctg ggtggaggtc
    43441 gctattaatc cgcgcgcccg ttttcatgat ggttcgccca ttactgcccg cgatgtagag
    43501 tttacttttc aaaaatttat gaccgaaggc gtgccgcaat ttcgtctggt ctacaaaggc
    43561 accaccgtca aagccattgc gccgttaacc gtgcgcattg agttagctaa acccggcaaa
    43621 gaagatatgt tgagtctgtt ttcgctgccg gtatttccag aaaagtactg gaaggatcac
    43681 aaacttagcg atccgctcgc cacgcctccg cttgccagtg gtccgtaccg aattacgtcc
    43741 tggaaaatgg ggcaaaatat tgtctattcc cgcgtgaaag attactgggc agcaaactta
    43801 ccggtaaacc gtggacgctg gaatttcgac accattcgct acgattatta cctcgatgat
    43861 aatgtcgcct ttgaagcgtt taaagcaggt gcctttgatt tgcgtatgga aaacgacgct
    43921 aaaaactggg ccacgcgtta taccggtaaa aatttcgata aaaaatacat catcaaagat
    43981 gagcaaaaga acgaatcagc ccaggatacg cgctggctgg cgtttaatat ccaacgtccg
    44041 gtattcagcg atcgccgggt ccgggaagca atcaccctcg cctttgactt tgaatggatg
    44101 aacaaggcgt tgttttacaa tgcctggagt cgcacgaaca gttattttca gaataccgaa
    44161 tacgcggcca gaaattaccc cgaagccgcg gagctggtgc ttctggcacc aatgaaaaaa
    44221 gatctaccgt cagaagtctt cacacaaatc taccagccgc cggtatctaa aggcgatggc
    44281 tacgatcgtg acaacctgtt aaaagccgac aaacttctca acgaagcggg ctgggtgctg
    44341 aagggtcagc aacgcgttaa tgccacaacg ggtcagccac tcagctttga attattgctt
    44401 cccgcaagca gcaatagtca gtgggtattg ccgttccagc acagcctgca acggctgggt
    44461 atcaacatgg acattcgcaa ggtggataac tcgcaaatca ccaaccgcat gcgcagtcgc
    44521 gactatgaca tgatgccgcg cgtatggcgg gcgatgccgt ggcccagttc cgatttacag
    44581 atttcctggt catcggaata tatcaattcc acttataatg cccccggcgt gcaaagtccg
    44641 gttatcgact cgctgatcaa tcaaattatt gccgcgcagg gaaataaaga aaaattactg
    44701 ccgttagggc gagcactgga tcgcgtatta acgtggaatt attacatgct gccaatgtgg
    44761 tacatggcgg aagaccgtct cgcctggtgg gataaattct cccaacccgc tgtacgccct
    44821 gtttacagcc tgggtatcga tacctggtgg tatgacgtta ataaagcggc caaactgccg
    44881 tcagccagac aacagggaga gtagatgggc gcttatctga ttcgccgtct gttgctggtg
    44941 atcccaacgt tatgggcgat tatcactatc aactttttca tcgtgcaaat tgcgcctggc
    45001 ggtccggtcg accaggccat cgccgccatt gagtttggta atgccggagt attacccggc
    45061 gcaggcggtg aaggtattcg cgccagccat gcgcaaacgg gcgtcggcaa tatcagcgac
    45121 agtaattacc gtggcggacg cggattagat ccagaagtga tcgccgagat cactcatcgc
    45181 tacggtttcg ataagccgat ccacgaacgt tacttcaaaa tgctctggga ctacatccgc
    45241 tttgattttg gtgatagcct ttttcgcagc gcctcggtgc tgacgctgat taaagacagt
    45301 ctgccggttt ccatcaccct cggattgtgg agcacgctga ttatctatct ggtgtcgatt
    45361 ccgttaggca ttcgcaaagc tgtttataat gggagccgct ttgacgtctg gagtagcgca
    45421 tttatcatca tcggctacgc cattccggcc tttttgtttg ccatcctgct gattgtcttc
    45481 ttcgcgggcg gcagctattt tgacctgttc cctctacgcg gcctggtttc cgctaacttt
    45541 gattcgctgc cgtggtatca gcaaatcacc gattatctgt ggcatatcac gctaccggtg
    45601 ctggcgacag tgattggtgg ctttgcggcg ctgaccatgc tgaccaaaaa ctcattcctt
    45661 gatgaagtgc gcaagcaata cgtggtgacc gcgcgtgcga aaggggtaag tgagaaaaac
    45721 attctctgga aacatgtgtt ccgcaacgcc atgctgctgg tgattgccgg ttttccggcg
    45781 acgtttatca gcatgttttt taccggctcg ctgctgattg aggtgatgtt ttcactcaat
    45841 ggtcttggct tactgggcta cgaagcgacc gtctcgcgcg attatcctgt aatgtttggt
    45901 accttgtata ttttcaccct gattggcctg ctgctgaata ttgtcagtga tatcagctat
    45961 acgctggtcg atccgcgtat agattttgag ggacgttaat gtcgcgactc agccccgtca
    46021 atcaggcccg ttgggcgcgt tttcgccata accgtcgcgg atactggtcg ttatggattt
    46081 tcctcgtctt gtttggtttg agtttgtgtt ctgaacttat cgctaacgat aaaccgttgc
    46141 tggtgcgtta tgacggcagt tggtatttcc cgttgttgaa aaactacagc gaaagcgatt
    46201 ttggcggccc gctggcaagt caggctgatt atcaggaccc gtggctgaaa caacggctgg
    46261 aaaataacgg ctgggtactg tgggcaccga ttcgctttgg tgctaccagt atcaactttg
    46321 ctaccgataa gcccttccct tctccaccct cccggcaaaa ctggctggga acggatgcca
    46381 acggcggcga tgtgctggca cgcattctct atggcacgcg gatctcggtt ctgtttggcc
    46441 tgatgctgac tctctgttcc agcgtgattg gcgtgctggc gggggcgcta caaggctatt
    46501 acggcggtaa agtcgatctc tggggacaac gctttattga agtatggtcg gggatgccga
    46561 cgctgttttt gattatttta ctttccagcg tcgtacagcc taacttctgg tggctgctgg
    46621 caattactgt cttgtttggc tggatgagtc tggtcggcgt ggtgcgggcg gagtttttac
    46681 gtactcgtaa tttcgactac attcgtgcgg cacaggcgct tggcgtcagc gatcgcagta
    46741 tcatcctgcg tcatatgttg cctaatgcca tggtcgcgac cctcaccttt ttaccgttta
    46801 ttttatgtag ttcgataacc accctgacct cgctcgattt cctcggcttc ggtctaccgc
    46861 tcggttcacc gtcactcggt gaactgctgt tacaagggaa aaataacctt caggccccgt
    46921 ggcttgggat caccgccttc ttgtcggtgg cgatattatt gtctttgctg atctttattg
    46981 gtgaagccgt ccgcgacgca tttgatccta ataaggcggt gtagcatgac gcaaactctg
    47041 ttagcgattg aaaatttgtc ggtgggtttt cgccatcagc aaaccgtacg tacagtagtc
    47101 aatgatgttt cactacagat tgaggctggt gaaacgctgg cgctggtggg tgagtcaggt
    47161 tcaggcaaaa gcgttaccgc gctgtcaatt ttacgcctgc tcccttcccc gccggttgaa
    47221 tatctctccg gcgatattcg ttttcatggc gaatcgctgc ttcatgtcag cgatcaaacg
    47281 ttgcgcggtg tacgcggtaa taagatcgcc atgatttttc aggaaccgat ggtgtcgtta
    47341 aatccattgc ataccctgga aaaacagctt tatgaagtgc tttcactcca ccgcgggatg
    47401 cgtcgggaag cggctcgtgg cgaaattctt aactgccttg atcgcgttgg tatccgccag
    47461 gcggcaaaac ggctgacaga ttatccgcat cagctctccg gcggcgaacg ccagcgggtg
    47521 atgattgcga tggcgctgtt aacgcgaccg gaattattaa ttgccgatga accgaccacc
    47581 gcactggacg tctctgtcca ggcgcagatt ttacagctgt tgcgcgaact gcaaggcgag
    47641 ctgaatatgg gcatgctgtt tattactcat aacctcagca ttgtcagaaa actggcccac
    47701 cgcgtggcgg taatgcaaaa cggtcgctgt gtcgagcaaa attacgccgc tacgctattt
    47761 gcatcaccca ctcatcctta cacacaaaag ctactcaaca gtgaaccgtc aggcgatcca
    47821 gtgccgttgc cagaacctgc ctcaacgttg ctggatgttg aacagcttca ggttgccttc
    47881 cccattcgca aagggatttt gaagcgcatt gtggatcata atgtggtggt gaaaaacatc
    47941 agttttacgc tacgagcggg tgaaacactg ggtttagtgg gcgagtccgg ttccgggaaa
    48001 agtacgacgg gactggcgct gctgcgactg attaattctc agggcagcat catctttgac
    48061 ggtcagccac tgcaaaattt aaatcgccgc cagctgttac ctattcgtca tcgcattcag
    48121 gtggtatttc aggatccaaa ctcctcgctc aacccacgac tcaacgtttt gcagattatt
    48181 gaggaaggct tacgggttca ccagccgacg ctttctgccg cacaacgcga acaacaagtg
    48241 atagccgtga tgcatgaagt gggattagat cctgaaacac gccaccgtta tccggcggag
    48301 ttctctggtg gtcagcgaca acgtattgcg attgccaggg cattaattct taagccctcg
    48361 ctgatcatac ttgatgaacc gacatcatca ctcgacaaaa cggtacaggc gcaaatattg
    48421 acgctattga aatcattgca acaaaagcat caactggcct atttgtttat cagccacgat
    48481 ttgcacgttg tccgcgcgtt atgtcatcag gttatcgtac tgcgacaagg ggaagtagtg
    48541 gaacaaggac cgtgcgcgcg cgtgtttgcc acaccgcagc aggagtatac gcgtcagcta
    48601 ctggcgttga gctgacgctt aaaaaggatt gtagtttgaa aagggttcgg caattgccac
    48661 accaaaattt ttcaatcggc atgttgcagc aaactcgtcc tggcgattta caaacaggca
    48721 cggctcacct tcacactcca gcaccgaaca tggaacttcg atgtcgctta acgcctggct
    48781 tagtttatac atcaccgacc atgcattatc accatccggg ctaagcaatt taagcgccac
    48841 caggctgttg tcaccgcacg gtccattttg cggaatcggt tcaaccttcg aacctgcgaa
    48901 accagcagac cagcgatagt tctgcggtag acgatggacg attgagagtt gtaatgaagt
    48961 cacttatttt ccccggaagt acatttactt cacaatttgt ttacatcaat tttaacacat
    49021 catcaacaat ccgtctttat acagatcgta aagattgatg cctcgcctga gtggcctcta
    49081 tggctctgat ttaagtaata tctgcgggcg tggtcacgtt tgcggggcta tatgtacccg
    49141 tttctgcg
//
LOCUS       DAPHDW010000019        47024 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.019, whole genome shotgun sequence.
ACCESSION   DAPHDW010000019 DAPHDW010000000
VERSION     DAPHDW010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 47024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47024
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.019"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..689)
                     /locus_tag="RH057_001044"
     CDS             complement(<1..689)
                     /locus_tag="RH057_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415390.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HDV3127453.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQVALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPASEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRR"
     gene            complement(700..1701)
                     /gene="ltaE"
                     /locus_tag="RH057_001045"
     CDS             complement(700..1701)
                     /gene="ltaE"
                     /locus_tag="RH057_001045"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HDV3127454.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYSCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALKNNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(1738..3456)
                     /gene="poxB"
                     /locus_tag="RH057_001046"
     CDS             complement(1738..3456)
                     /gene="poxB"
                     /locus_tag="RH057_001046"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HDV3127455.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNHVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPIVTPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGTPTVWAARYLK
                     MNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLMGDFLSVVQMK
                     LPVKIVVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITGIRVEKASEVD
                     EALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIISGRGDEVIELA
                     KTNWLR"
     gene            complement(3589..4557)
                     /gene="hcr"
                     /locus_tag="RH057_001047"
     CDS             complement(3589..4557)
                     /gene="hcr"
                     /locus_tag="RH057_001047"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HDV3127456.1"
                     /translation="MTMPTNQCPWRMQVHHITQETPDVWTISLICHDYYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLAGVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(4569..6221)
                     /gene="hcp"
                     /locus_tag="RH057_001048"
                     /gene_synonym="priS"
     CDS             complement(4569..6221)
                     /gene="hcp"
                     /locus_tag="RH057_001048"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HDV3127457.1"
                     /translation="MFCVQCEQTIRTPAGNGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGDDFSAVITQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLSA"
     gene            complement(6365..7264)
                     /gene="lysO"
                     /locus_tag="RH057_001049"
     CDS             complement(6365..7264)
                     /gene="lysO"
                     /locus_tag="RH057_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HDV3127458.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKVINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
     gene            complement(7721..8416)
                     /gene="aqpZ"
                     /locus_tag="RH057_001050"
     CDS             complement(7721..8416)
                     /gene="aqpZ"
                     /locus_tag="RH057_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin Z"
                     /protein_id="HDV3127459.1"
                     /translation="MFRKLAAECFGTFWLVFGGCGSAVLAAGFPELGIGFAGVALAFG
                     LTVLTMAFAVGHISGGHFNPAVTIGLWAGGRFPAKEVVGYVIAQVVGGIVAAALLYLI
                     ASGKTGFDAAASGFASNGYGEHSPGGYSMLSALVVELVLSAGFLLVIHGATDKFAPAG
                     FAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWALEQLWFFWVVPIVGGII
                     GGLIYRTLLEKRN"
     gene            8842..10500
                     /locus_tag="RH057_001051"
     CDS             8842..10500
                     /locus_tag="RH057_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HDV3127460.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(10497..11453)
                     /locus_tag="RH057_001052"
     CDS             complement(10497..11453)
                     /locus_tag="RH057_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HDV3127461.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRRSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            11604..12719
                     /gene="macA"
                     /locus_tag="RH057_001053"
     CDS             11604..12719
                     /gene="macA"
                     /locus_tag="RH057_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HDV3127462.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSQQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            12716..14662
                     /gene="macB"
                     /locus_tag="RH057_001054"
     CDS             12716..14662
                     /gene="macB"
                     /locus_tag="RH057_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HDV3127463.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGITLDIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDVATLDADALAQLRREHFGFIFQRYHLLSHLT
                     AEQNVEVPAVYAGLERKQRLLRAQGLLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(14735..14959)
                     /gene="cspD"
                     /locus_tag="RH057_001055"
     CDS             complement(14735..14959)
                     /gene="cspD"
                     /locus_tag="RH057_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HDV3127464.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            15282..15602
                     /gene="clpS"
                     /locus_tag="RH057_001056"
     CDS             15282..15602
                     /gene="clpS"
                     /locus_tag="RH057_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HDV3127465.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            15633..17909
                     /gene="clpA"
                     /locus_tag="RH057_001057"
     CDS             15633..17909
                     /gene="clpA"
                     /locus_tag="RH057_001057"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HDV3127466.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     NLGDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPHAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(18253..18340)
                     /locus_tag="RH057_001058"
     tRNA            complement(18253..18340)
                     /locus_tag="RH057_001058"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18304..18306),aa:Ser,seq:gga)
     gene            complement(18594..18812)
                     /gene="infA"
                     /locus_tag="RH057_001059"
     CDS             complement(18594..18812)
                     /gene="infA"
                     /locus_tag="RH057_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HDV3127467.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(19097..19801)
                     /gene="aat"
                     /locus_tag="RH057_001060"
     CDS             complement(19097..19801)
                     /gene="aat"
                     /locus_tag="RH057_001060"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HDV3127468.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(19843..21564)
                     /gene="cydC"
                     /locus_tag="RH057_001061"
     CDS             complement(19843..21564)
                     /gene="cydC"
                     /locus_tag="RH057_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HDV3127469.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGGIMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRITDLTDQKPEVTFPDTQTHVA
                     NRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDETLAEIL
                     RRVGLEKLLEDTGLNSWLGEGGRQLSGGELRRLAIARALLHDAPLVLLDEPTEGLDAT
                     TESQILELLAEMMHEKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(21565..23331)
                     /gene="cydD"
                     /locus_tag="RH057_001062"
     CDS             complement(21565..23331)
                     /gene="cydD"
                     /locus_tag="RH057_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HDV3127470.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGETEIASTDPVTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAATLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(23454..24419)
                     /gene="trxB"
                     /locus_tag="RH057_001063"
     CDS             complement(23454..24419)
                     /gene="trxB"
                     /locus_tag="RH057_001063"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HDV3127471.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMERMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            24964..25458
                     /gene="lrp"
                     /locus_tag="RH057_001064"
     CDS             24964..25458
                     /gene="lrp"
                     /locus_tag="RH057_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HDV3127472.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            25593..29660
                     /gene="ftsK"
                     /locus_tag="RH057_001065"
     CDS             25593..29660
                     /gene="ftsK"
                     /locus_tag="RH057_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HDV3127473.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EIIYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNGAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAAEQPVQQPYYAPAPEQPVAGNAWQAE
                     EQQSTFAPQSTYQTEQTYQQPAAQEPLYQQPQSVEQQPVVEPEPVVEETKPARPPLYY
                     FEEVEEKRAREREQLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLAS
                     GVKKATLATGAAATVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYG
                     IKLPSQRAAEEKAREAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQH
                     DVPVNAEDADAAAEAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGP
                     HEPLFTPIVEPVQQPQQPVAPQQQYQQPQQPVPPQQQYQQPQQPVAPQPQYQQPQQQV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQYQQPQQPVAPQQQDTLLHPLLMRNGDS
                     RPLHKPTTPLPSLDLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGP
                     VITRFELNLAPGVKAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTV
                     YLREVLDNAKFRDNPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAM
                     ILSMLYKAQPEDVRFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMER
                     RYKLMSALGVRNLAGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLV
                     DEFADLMMTVGKKVEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAF
                     TVSSKIDSRTILDQAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKA
                     RGRPQYVDGITSDSESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIG
                     YNRAARIIEQMEAQGIVSEQGHNGNREVLAPPPFD"
     gene            29815..30426
                     /gene="lolA"
                     /locus_tag="RH057_001066"
     CDS             29815..30426
                     /gene="lolA"
                     /locus_tag="RH057_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HDV3127474.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            30437..31780
                     /gene="rarA"
                     /locus_tag="RH057_001067"
                     /gene_synonym="mgsA"
     CDS             30437..31780
                     /gene="rarA"
                     /locus_tag="RH057_001067"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HDV3127475.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDETRRAIAELVNGDARRALNTLEMM
                     ADMAEVNDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            31871..33163
                     /gene="serS"
                     /locus_tag="RH057_001068"
     CDS             31871..33163
                     /gene="serS"
                     /locus_tag="RH057_001068"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HDV3127476.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            33402..35846
                     /gene="dmsA"
                     /locus_tag="RH057_001069"
     CDS             33402..35846
                     /gene="dmsA"
                     /locus_tag="RH057_001069"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
                     /protein_id="HDV3127477.1"
                     /translation="MKTKIPDAVLAAEVSRRGLVKTTAIGGLAMASSALTLPFSRIAH
                     AVDSAIPTKSDEKVIWSACTVNCGSRCPLRMHVVDGEIKYVETDNTGDDNYDGLHQVR
                     ACLRGRSMRRRVYNPDRLKYPMKRVGARGEGKFERISWEEAYDIIATNMQRLIKEYGN
                     ESIYLNYGTGTLGGTMTRSWPPGNTLVARLMNCCGGYLNHYGDYSSAQIAEGLNYTYG
                     GWADGNSPSDIENSKLVVLFGNNPGETRMSGGGVTYYLEQARQKSNARMIIIDPRYTD
                     TGAGREDEWIPIRPGTDAALVNGLAYVMITENLVDQAFLDKYCVGYDEKTLPASAPKN
                     GHYKAYILGEGPDGVAKTPQWASQITGIPAEKIIQLAREIGSTKPAFISQGWGPQRHA
                     NGEIATRAISMLAILTGNVGINGGNSGAREGSYSLPFVRMPTLENPIQTSISMFMWTD
                     AIERGPEMTALRDGVRGKDKLDVPIKMIWNYAGNCLINQHSEINRTHEILQDDKKCEL
                     IVVIDCHMTSSAKYADILLPDCTASEQMDFALDASCGNMSYVIFNDQVIKPRFECKTI
                     YEMTSELAKRLGVEQQFTEGRTQEEWMRHLYAQSREAIPELPTFEEFRKQGIFKKRDP
                     QGHHVAYKAFREDPQANPLTTPSGKIEIYSQALADIAATWELPEGDVIDPLPIYTPGF
                     ESYQDPLNKQYPLQLTGFHYKSRVHSTYGNVDVLKAACRQEMWINPLDAQKRGINNGD
                     KVRIFNDRGEVHIEAKVTPRMMPGVVALGEGAWYDPDAKRVDKGGCINVLTTQRPSPL
                     AKGNPSHTNLVQVEKV"
     gene            35857..36474
                     /gene="dmsB"
                     /locus_tag="RH057_001070"
     CDS             35857..36474
                     /gene="dmsB"
                     /locus_tag="RH057_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HDV3127478.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            36476..37339
                     /gene="dmsC"
                     /locus_tag="RH057_001071"
     CDS             36476..37339
                     /gene="dmsC"
                     /locus_tag="RH057_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit
                     DmsC"
                     /protein_id="HDV3127479.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLIVTMVLGVIFVWMMVRVYNSIDTVPTWYSIWTPMGFFLTMF
                     MGGPLLGYLLLSLAGVDGWAMRLLPAISVLALVVSGVMSVMQGTELATIHSSVQQAAA
                     LVPDYGALMSWRIVLLAVALCLWIAPQLKGYQPAVPLLSVSFILLLAGELIGRGVFYG
                     LHMTVGMAVAS"
     gene            complement(37375..38001)
                     /locus_tag="RH057_001072"
     CDS             complement(37375..38001)
                     /locus_tag="RH057_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV3127480.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVPELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            38316..39464
                     /locus_tag="RH057_001073"
     CDS             38316..39464
                     /locus_tag="RH057_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3127481.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLASFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSYTVGS
                     LLGPSFTAMLMQNFSDNLLFIMIASVSFIYLLMLLRNAGHTPKPVAHV"
     gene            39674..41104
                     /locus_tag="RH057_001074"
     CDS             39674..41104
                     /locus_tag="RH057_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3127482.1"
                     /translation="MAGNVQEKQLRWYNIALMSFITVWGFGNVVNNYANQGLVVVFSW
                     VFIFALYFTPYALIVGQLGSTFKDGKGGVSTWIKHTMGPGLAYLAAWTYWVVHIPYLA
                     QKPQAILIALGWAMKGDGSLIKEYSVVALQGLTLVLFIFFMWVASRGMKSLKIVGSVA
                     GIAMFVMSLLYVAMAVTAPAITEVHIATTNITWETFIPHIDFTYITTISMLVFAVGGA
                     EKISPYVNQTRNPGKEFPKGMLCLAVMVAVCAILGSLAMGMMFDSRNIPDDLMTNGQY
                     YAFQKLGEYYNMGNTLMVIYAIANTLGQVAALVFSIDAPLKVLLGDADSKYIPASLCR
                     TNASGTPVNGYFLTLVLVAILIMLPTLGIGDMNNLYKWLLNLNSVVMPLRYLWVFVAF
                     IAVVRLAQKYKPEYVFIRNKPLAMTVGIWCFAFTAFACLTGIFPKMEAFTAEWTFQLA
                     LNVATPFVLVGLGLIFPLLARKANSK"
     gene            complement(41105..42013)
                     /locus_tag="RH057_001075"
     CDS             complement(41105..42013)
                     /locus_tag="RH057_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3127483.1"
                     /translation="MRMNMSDFATFFAVARNQSFRAAGDELGLSSSAISHSIKTLEQR
                     LKIRLFNRTTRSVSLTEAGSNLYERLRPAFDEIQIMLDEMNDFRLTPTGTLKINAARV
                     AARIFLMPLLVGFTREYPDIKVELTTDDSLVDIVQQGFDAGVRLSGIVEKDMISVAIG
                     PPVKLCVAATPEYFARYGKPRHPHDLLNHQCVVFRYPSGKPFHWQFAKELEIAVAGNI
                     ILDDVDAELEAVLMGAGIGYLLYEQIKEYLDTGRLECVLEDWSTERPGFQIYYPNRQY
                     MSCGLRAFLDYVKTGQICQSQRHRPQ"
     gene            42113..42703
                     /locus_tag="RH057_001076"
     CDS             42113..42703
                     /locus_tag="RH057_001076"
                     /EC_number="1.6.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H oxidoreductase"
                     /protein_id="HDV3127484.1"
                     /translation="MQSERIYLVWAHPRHDSLTAHIADAIHQRAMERKIQVTELDLYR
                     RNFNPVMTSEDEPDWKNMDKRYSPEVHQLYSELLEHDTLVVVFPLWWYSFPAMLKGYI
                     DRVWNNGLAYGDGHKLPFNKVRWVALVGGDKESFVQMGWEKNISDYLKNMCSYLGIED
                     ADVTFLCNTVVFDGEELHASYYQSLLSQVRDMVDAL"
     gene            complement(42785..43525)
                     /gene="pflA"
                     /locus_tag="RH057_001077"
     CDS             complement(42785..43525)
                     /gene="pflA"
                     /locus_tag="RH057_001077"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HDV3127485.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(43717..45999)
                     /gene="pflB"
                     /locus_tag="RH057_001078"
     CDS             complement(43717..45999)
                     /gene="pflB"
                     /locus_tag="RH057_001078"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV3127486.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKQLEKIVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKLAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLNYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(46054..46911)
                     /gene="focA"
                     /locus_tag="RH057_001079"
     CDS             complement(46054..46911)
                     /gene="focA"
                     /locus_tag="RH057_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HDV3127487.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWGQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
BASE COUNT        11619 a        11695 c        12429 g        11281 t
ORIGIN      
        1 cggcgcttac cgctcaccgc cataaaatgt tcaaactgtt gctgataact gagcacctct
       61 ggtccggcgg cttcgaagat gcggtgttcg ctggccggat gatctaacaa cgccaccaga
      121 tagtgcagca agttttccag cgcgatgggc gtggtgcgtg aacgtaccca gcgtggcggc
      181 gttaacactg gcaggttgta gaccatatcg cgcatgactt cgaacgccgc tgaacctgcg
      241 ccaacgataa ttccggcccg aagttcggtc acaggtacat tcgcttcacg aagaatgtcc
      301 gccgtagcct gacgagcacg cagatgatcc gactgctcat gtggcggggc ctgcaacgaa
      361 ctgagaaaga ttaattgctt aactggtact tcacgtagcg catcgcggac gttgagagcc
      421 acctggcgct cctgagcgat aaaatcgccg ccttcgccca tgctgtgcac cagaaaatag
      481 acggtatcga tatcctgcaa cagggccgga aggttatccg gccagctgag atcgacttta
      541 tggcaactga cgtttgccag ttgcagcttt gcaagcctgt cgacatgacg tgccgccgcc
      601 aggatctgat gcccttgctg gctgagtgtg cgcaccagat gctgaccaat gtagccactg
      661 gcaccgagaa ctaaaatgcg ttgcggcacg tctctctcct taacgcgcca ggaatgcgcg
      721 ccagtgggcg gcgacttccg ccagttgttc gcgcgagacg tcaagatgcg tcaccaggcg
      781 gacaatcggc gaggcgttaa tcagcacgtt tctcgctttc atgtattcgc ctaacgcggc
      841 agcattttct tccccgacgc gaacaaacag catattagtg tcctgacgca tcacatccgc
      901 gcctgcttca cgcagttgct ccgccatcca ggcggcgttg tcgtgatctt cctgcaaccg
      961 tgcgacgtta tttttcaggg catacatccc ggcggcagcc agaatcccgg actgacgcat
     1021 cccgccaccg gccattttcc gccagcggat ggcacgttta atgtaatcac gattaccgac
     1081 gagtaatgaa ccgactggcg tcccaagacc tttcgacagg caaatggtga acgaatcaca
     1141 atattgcgtg atctctttca gttcgcagct gtaagccacc acggcattaa agatgcgcgc
     1201 accgtcaaca tgcagcgcca gattgcgctc gcgggtaaat tcccatgctt ctttcaggta
     1261 ttcacgcggc agcactttgc cgttgtgggt gttttccaga ctgagtaatt tggtgcgggc
     1321 gaaatggata tcgtcgggtt tgattttcat cgccacttta tccagcggta gcgtgccgtc
     1381 ggcagccgcg tcgatgggtt gcggctgaat actgcccagc accgctgcgc caccggcttc
     1441 aaacagatag ttatgcgcgg cctgaccgac aatatactct tcgccacgtt cgcagtgact
     1501 gagcagagcg accaggttgg cctgagtgcc ggtaggcaga aaaatggcgg cttctttacc
     1561 ggaaagctct gctgcatagt cctgcagagc attaacggta gggtcgtctc cgtaaacgtc
     1621 gtccccaacc ggggcggcca tcatcgcttc gagcatggcg cggctcggtc gggtaacggt
     1681 atcactgcgt aaatcaatca tggcatgtcc ttattatgac gggaaatgcc acccttttta
     1741 ccttagccag ttcgttttcg ccagttcgat cacttcatcg ccgcgtccgc tgataattgc
     1801 gcgcaacata tacaggctaa aacctttggc ctgttcgagt ttgatctgcg gtggaatggc
     1861 taactcttct ttggcgacca ccacatccac caacaccgga ccgtcgatag agaaggcgcg
     1921 ttgcagggct tcatcaactt cagacgcttt ttctacacgg atacccgtaa tgccgcacgc
     1981 ttcggcaatg cgggcaaagt ttgtgtcgtg tagttcggtg ccgtcagtca aatagccacc
     2041 agctttcatc tccatcgcca caaagcccag cacgctgttg ttaaagacga caattttcac
     2101 tggcagtttc atctgcacta ctgagaggaa atcgcccatc aacatgctaa aaccgccatc
     2161 gccgcacatg gcgaccacct gacgttctgg ctctgtcgcc tgcgcaccca gcgcctgcgg
     2221 catggcgtta gccatcgaac cgtggttaaa cgaacctaac aggcgacgct tgccgttcat
     2281 tttcagataa cgtgccgccc ataccgttgg cgtgccgacg tcacaggtaa agatggcgtc
     2341 atcggcggca aaatgactaa tttgctgcgc cagatattgc gggtgaatgg ctttctcgct
     2401 cggtttagct aaatcgtcca gccctttgcg ggcatcgcgg taatcttcca gcgctttatc
     2461 cagaaacttg cgatcggttt tttcttccac cagtggcagc aatgcgcgca gggttgattt
     2521 gatatcgccg accagcgcca tatctacctt gctatgcgcg ccgatgctgg ctggattgat
     2581 atcaatctga ataattttgg catcggtcgg gtagaacgca cggtagggga actgagtacc
     2641 gagtagcact aacgtgtcgg cgttcatcat ggtatggaaa cctgacgaga agccgattaa
     2701 cccggtcatt ccaacatcat acggattatc gtattcgaca tgttctttac cgcgcagggc
     2761 atgaacaata ggcgctttaa ttttcccggc aaactcaact aactctttat gcgcccccgc
     2821 gcagccgctg ccacacatca gggcgatatt gctggaataa cgcagcagtt gcgccagttt
     2881 gcgtaactct tcttcttccg gcgtcacgat tggttgtggc gcatgatacc agtgcgtggt
     2941 tgccccttct ggcgcaggtt ttaacgccac gtcgcctggt aacacgacaa ccgaaacgcc
     3001 acggttaagc accgctttgc gcatggcaat cgccagtact tgtggaatct gctccgggct
     3061 ggaaaccagc tcgcaatagt gactacattc gcggaatagc tcttgtgggt gggtttcctg
     3121 gaaatagccg ctgccaattt cgctggaggg aatatgagcg gcaatcgcca gtaccggaac
     3181 gtgattgcgg tggcaatcga acaggccgtt gattaagtgc aggttgccgg ggccgcacga
     3241 tccggcacag accgccagtt ctccgctaag ttgtgcttca gcgccagcgg caaaggccgc
     3301 cacttcttcg tggcgggtgg acatccactc gatggtgccc atgcgattaa gactgtcact
     3361 aagaccgttc agagagtcgc ctgtgactcc ccagatgcgt ttcacccctg ccgattcgag
     3421 tgttttggcg atataagctg caaccgtttg tttcatggtt ctccatctcc tgaatgtgat
     3481 aacggtaaca agtttagttc atctgacgga gggggaaggg atgggagaga aaggaggcac
     3541 taacggttaa atagcccgat gaaaggaata tcatcgggca taaggcgatt atgcgagaac
     3601 caaatccccc tgcggatggc aggagcaggc cagtacgtaa ccttcagcga tttcggcgtc
     3661 ggtcagcgtc attgtactgc tcaccgtata ttcaccggaa accacttttg tcttacagca
     3721 gccgcaaaca cccgcacggc aggcagcgac aaccggaacg ttattgcttt ccagcgcctc
     3781 cagtagcgtg gtgccaaccg gggcgtaaaa ttctcgtgcc ggttgcagtt tggtgaattt
     3841 cagaccgctg gtcgccgctt ctgctactgg ggtgaagaat ttctctttaa agaaacgcgt
     3901 cacgccgagc gctttcactt cctgctctac ccaatccata tacggagccg ggccgcaggt
     3961 catcacggta cgtgaagcta agtcaggtac acctgccagc agttcgcgag tgagacgacc
     4021 agcgataaag ccttcggtaa cgttattttc tgccaccagc gttaccggat agttacgcca
     4081 ctcatcggcg aaaataacat cctgcggcgt acgcacgttg tagatcaccc gcacatcggc
     4141 ctgtggacgg ttcttcgcaa gccagcgacg catcgacata atcggcgtga cgccgcagcc
     4201 tgccgccagc aacaggaatt tatcttctgc tttatcgtcg caggtaaatt cccccatcgc
     4261 gtccgaaagc cagagataat caccgcgttt tacatcgcgc gtcagccact gggagccgac
     4321 accgtcatca atccgccgca cggtcagggt gatatattca ctcacgcctg gcgtggagga
     4381 aatggtgtaa gcacgcagcg tttccgctga gtttcgcacg ctgaccagtg catattgccc
     4441 ggcgcgatat gggtagtaat cgtggcaaat cagggaaatc gtccacacat ccggcgtttc
     4501 ttgcgtaatg tgatgaacct gcatccgcca cgggcattga ttcgttggca tcgtcatcga
     4561 caaactcctt acgcgctcaa cagttgcttc atgtcttctt caacagtggt gatagaacgc
     4621 aggccaaatt tctcgttcag caccgccagc aggtctggtg tcaggaaacc aggtgcagtc
     4681 gggccggtga cgatattttt cacgcccaga gaaagcagcg tcagcagaat gacgatcgct
     4741 ttctgttcaa accaggagag caccagcgac agcggcagat cgttgacacc gcagcccagt
     4801 ttctctgcca gagtgacagc cagaataatc gctgagtaag catcgttaca ttgacctgca
     4861 tctaccagac gcggcagacc ttcgatatcg ccaaactcaa gtttgttaaa gcgatattta
     4921 ccacaggcga gggtcaggat caggcagtca tccggcacgc tggtggcgaa atcggtgaag
     4981 tagtggcgct cgccgcgtgc gccgtcacag ccaccaagca ggaagatatg acgcagtttt
     5041 tcacggctca ccagatcaat cagcgtatca gcagcgccaa gcagcgtctg gcgaccaaaa
     5101 cccacggtga taaggtgcgg aatttcgctg tacgggaagc ctgccatctg ttgcgcctgg
     5161 gtgataaccg cagagaaatc atcaccgtcc agatgacgca cgccaggcca gccaacaatg
     5221 ctgcgggtcc agatacgatc gtcataagcg cctacggttg ggtcgatgat gcagttcgag
     5281 gtcatcacga tggggccagg gaaacgagcg aactccactt gctgattctg ccagccactg
     5341 ccgtagttac cgaccagatg cttgaattta cgcagctccg ggtagccatg cgcaggcagc
     5401 atttcgccgt gggtgtagac attaacgccc gtgccttcgg tctgttccag caggttgtag
     5461 agatctttga gatcgtgacc ggaaatcagg atgcatttac ccgccgtcgc tttgacgttg
     5521 acctgggttg gcgtcgggtg accgtattta ccggtttcgc ctgcatccag aatgctcatc
     5581 actttgaagt tcatctggcc gatttccatt gaacactcaa gaagcgcgtt catatcggca
     5641 ggccaggtcc ccagccacgc catgatttta tgatactggg cataaatatc gttgtcgtat
     5701 tgaccgagaa catgcgcgtg ttccatatag gccgctgcac ctttcaggcc atacaggcac
     5761 agcagacgca ggccgagaat gttttcgcca atcgccgctt tatctttgtt aggggtaaat
     5821 tctgctgcct gacgttgcag ctcgccgaga tcgtcgctca ccagttgcag gtcagccatc
     5881 gggttatcga cgcgcgcgtt ggcatctaca gccaggcatt gcgctttcag cgcctcgcgc
     5941 agggcaatcg cttcacgagc gtagccgaca atacgcggag aatcgaagtt aacgttggtc
     6001 agggttgaga aaaaggcacg tggcgcgaag ctgtcaacat cgtggttgat gatgccgtat
     6061 tcacgcgctt ttaccgccca ggcagaaagc ccttgcagcg ccgcgatgag taaatcctga
     6121 aggtcagaag tttccgccgt tttaccacac atcccctgcg cgtatgagca gccgtttcct
     6181 gccggagtac ggatagtttg ttcacattgc acacaaaaca tgatcacacc ttttaaagtt
     6241 atatttaata tacatgttta aggttaagac gcttaacgcg gggataaaag ggatttttca
     6301 tgcaacttta agggagattg atttagcgca attttggcgg cagggatcta ccgccagaga
     6361 ggtattacgc agagaaaaag gcgatgagga tcggcactaa caggctaaga ataaaaccgt
     6421 gaacaattgc cgccgggacc atatccagcc cgccagtacg ttgaagaacg ggcagggtga
     6481 aatccattga tgtggcaccg cataagccca gtgcagtaga gcggctgcgg cgaatcagcc
     6541 cagggatcaa cataatagca atcagttcac gggccagatc attaaaaaac gccgcgctcc
     6601 cgattaccgg accaaaagat tcggtcaata aaataccgga aagagaatac cagccgaaac
     6661 cggaggccat tgccagcgcg gtattgatgg ggagatcaag aataaaggcg ttaattaaac
     6721 caccaattaa tgaactgaca accaccacca cggcgacaat cattccccgg cgattaagga
     6781 caatctgctt taaggtcatg ccattattgc gcaactgaat accaacgagg aaaagtagca
     6841 aaattaacgt gtattcactg gcttcggtcg cgtgttgtaa gaaagccagt ccactcagac
     6901 caatggcaaa accaatcact actacgccgc acagttttag cgactccagc gccatcgcaa
     6961 tacgcgacgg gagtttttct tgctgatggt ggttgcgcca cggcaggcct cgctccagcc
     7021 acatcagggc ggcaatatta cacagtaaaa taacggtaat actgacggca gaataatgca
     7081 gaatcgccaa caggttactg gcgaggttat cgagaaacgc cagactgata cccataaaaa
     7141 agagaataag gtaaaccatc cagcttaata gctgattaat aacttttaac gcagcttgtt
     7201 ggcgaagcgg aatgaggtaa cccacaatca ggggaaccag aatgattaac agcccagaaa
     7261 acatgaaaac ccagtccttg caaagatgaa gtcgaaatgc gcgatgacac actactgaaa
     7321 gcggaaggac gagtaaagtt gcaattaaaa ggaaatgtta tgcatcagag ggggattttt
     7381 aatcctatct gagtgtttag cattgttgcg ctcctttcat gtgcaatgcc atataaagcc
     7441 actggcgcag ggagcgcgca gggggcggcc agtcgccgcc gccccctgca cccccgggct
     7501 ctggcgaaca aaatcgccgc tgcgcggtac cttcagctta tccccttcgg ctaccgggtc
     7561 gggcgcgagg taacgtcctg taagacgcgc cctcagccca catccatgtg ggctgccccg
     7621 gccttcgggg aacgcttcag cgattttgac gccaccaaca cccgtgcggt ttgtaacgtc
     7681 gaagataaaa caaccagggc agataaaatg ccaatgctaa ttaattacgt ttttccagca
     7741 gggtccggta aatcagacca ccgataatgc cgccgacaat tggcaccacc cagaagaacc
     7801 acagttgttc taatgcccag ccgccctgga agatagcaac cgcggtgctg cgcgccgggt
     7861 taacagaagt gttagtcacc ggaatactaa ttaagtgaat cagggttaag gccagaccaa
     7921 tagcgatcgg cgcaaaacct gccggcgcga atttgtcggt tgcgccgtgg atcaccaaca
     7981 ggaaacctgc actcaatacc agttcaacta ccagcgcgga aagcatggaa taaccgcctg
     8041 gtgaatgctc gccataaccg ttagaagcaa aaccgctggc tgccgcgtca aaacccgttt
     8101 taccactggc aattaaatac agcagcgccg ctgcaacaat accgccgaca acctgggcaa
     8161 ttacgtagcc aacgacttct tttgccggaa aacgtccgcc agcccataaa ccaatagtga
     8221 ccgccgggtt aaaatgacca ccagaaatat gaccaacagc aaaggccatc gtcagaacgg
     8281 tcagaccgaa cgccaacgcc acgccggcaa aaccaatgcc taattccggg aagcctgcgg
     8341 ccagtacagc actaccacag ccaccaaaaa caagccagaa agtaccaaaa cattcagctg
     8401 ctaattttct gaacatatcc acctcaatta aaaattgacc ctgtgaaaaa tatggtcgtt
     8461 ttatagggtc gtcgtaaaaa agtgacgacg gaaataatgc gcggctattt taaaaacgaa
     8521 ggcgagtcat tcaccagata aataaatcca gtaaatttga tttagggcaa cagcgggttg
     8581 ccccatatag tcatttgtct gattgacagt gtagtgcaag caaaagattt aatcctttag
     8641 gcgtaataaa aaataattta tcatgctaat tatttgattt tgttgttttt gcagacttat
     8701 cagcaagagg gagtataacg cgattattcg ctcatttttc agacatttgc catgcttaaa
     8761 tgtgatgtca tcacgtatta gcaaggcctt tcccgttata ctgccagcgt aaaggataag
     8821 tcacatattt ctggagggga tatgattctt gagcgcgttg aaattgtggg ttttcgcggt
     8881 atcaaccgtt tgtcgttgat gctcgaacaa aacaacgtcc tgattgggga gaacgcgtgg
     8941 ggtaaatcca gcttgctgga cgccttaacc ctgctgctat cgccagaatc agatctctac
     9001 cattttgagc gcgacgattt ctggttcccg ccgggagata tcaacgggcg agaacatcat
     9061 ctgcatatta ttttgacctt ccgcgaatcg ctgccaggcc gacatcgggt tcgccgttat
     9121 cggccgctgg aagcgtgctg gacgccatgc accgatggct atcaccgtat tttttatcgt
     9181 ctggaagggg agagtgcgga agacggcagc gtgatgacac tgcgcagttt tctcgataaa
     9241 gacggacatc cgattgatgt cgaggatatt aacgatcagg cacgccatct ggtgcgttta
     9301 atgccggtgc tgcgcttgcg tgatgcccgt tttatgcgcc gtattcgtaa cggcacggtg
     9361 ccaaatgttc ccaatgtgga agtcaccgcg cgccagctcg atttcctcgc ccgtgagttg
     9421 tcctcacatc cgcaaaatct ctctgatgga cagattcgtc agggactttc cgcaatggtg
     9481 cagttgcttg agcattattt ctctgagcag ggggctggac aggcgcgata tcgtttaatg
     9541 cggcggcggg ccagcaatga gcaacgaagc tggcgctatc tggatatcat caaccggatg
     9601 attgaccgac ctggagggcg ctcgtatcgg gttattttgc tcggcctgtt tgcaacttta
     9661 ttgcaggcaa aaggcacatt gcgactggat aaagacgccc gtccattgtt gctgattgaa
     9721 gatccagaaa cccgtttaca ccccattatg ctttcagttg cctggcatct gttgaatctt
     9781 ctgccattgc agcgcattgc caccaccaac tcgggtgagt tgctttcgtt aactccagtg
     9841 gaacatgttt gccgcctggt gcgtgaatct tctcgtgttg ccgcctggcg tctggggcca
     9901 agtggcttga gcaccgaaga cagccgacgc atatcctttc acattcgttt taaccgtccg
     9961 tcatcgctgt ttgcacgctg ctggttgctg gtggaagggg aaacggaaac ctgggttatc
    10021 aatgaactgg cgcgtcagtg cggacatcat tttgatgccg aagggatcaa ggtcattgag
    10081 tttgcccagt ccgggctaaa gccactggtt aaatttgccc gccgaatggg gattgaatgg
    10141 catgtactgg tcgatggcga tgaagcaggg aagaaatatg ccgctacggt acgcagcctg
    10201 ttgaataacg atcgggaagc cgaacgagaa catttaacgg cgttaccggc gctggatatg
    10261 gaacatttta tgtatcgcca gggattttcc gatgtgttcc accgcgtggc gcaaatcccg
    10321 gaaaatgtac cgatgaatct acgcaaaatt atctcgaaag cgatccatcg ctcttccaaa
    10381 cccgatcttg ccattgaagt ggcaatggag gcaggacgtc gtggtgtgga ctccgtaccg
    10441 acgctgctga aaaaaatgtt ctcacgcgtg ctgtggctgg cgcgcggtcg cgcggattaa
    10501 ccgcgaaaca tcgtggccat ttgtggctga atagcgtcga gcatctcata gcgccgacgg
    10561 tattcagccc gttttttact ggcgatttcg gcaatctctt ttcgtgctat ctgtgctgga
    10621 aggcggtaat ggcgttcagc atcacatacg ccgccaaccg attcccagaa agcgttgtaa
    10681 tcagcgtgga tcttgccttc tttatcgcga taacgcaggc tgcggtaaat atgcgtttca
    10741 ttgctgacgg caataatctg ctctacctgc aaacgttggg caaacagaca ggccgcttcc
    10801 atcacgaggc gtttgggaaa tagcccgtgg caggctttcg tcgcattctg gatttcctga
    10861 tgtggaattt cccattttgc gccttgcagt ccgccaataa acatcgttct tttcccctga
    10921 tattcacaca gggtaaacgt gatctctgcc agaggaatac cttcgctgtt gcggaacagg
    10981 attgtgctgt caccttcttt atccattgag atcatcatgg tcagctcaag cgtgaactgc
    11041 tcgccgtttt tgccttccag cttcgccagt tgcagcccgg gggtattcaa atataagctg
    11101 aattcttccg ccgacataca tccgcggagt aacgcataat ggtaacgtaa cgcctccagc
    11161 aattgcttac ggctaagatt cgccgcaagg taagggcgat gcagacgcac aggcagtcgc
    11221 ggctggcgcg ttaacaatac attgagatta ggccagtggg aaagttcgtt catccactca
    11281 acgcttaaac gcggcataat caacgagcgc agcaaaaatt tctggcgaaa actacggcga
    11341 tgccagaatt tacccggccg acactgtcca cgtgccagac taagaaaaag tgacaggctg
    11401 ctgagagatt cagatggcgt aaaggtccgt tcagttagct gcgacatatt catgaaatca
    11461 atggttatac atgacgtcga tttcaccatt gcgtatctta accaaacatc aatagtgtga
    11521 ttactaacgt aaattttagg ggtttgttga tatttcgttg aagttaatga cccggattgg
    11581 catatggagt attcagaata tttatgaaaa agcggaaaac cgtgaagaag cgttacgtta
    11641 ttgcgctggt gatagtcatc gccggactga ttacgttatg gagaattctt aacgcacccg
    11701 tgccgactta tcagacactg attgtgcgcc ccggtgattt acagcaaagc gtgctggcga
    11761 ccggaaagct ggacgcgctg cgtaaggttg acgtgggcgc gcaggtcagc ggtcagttga
    11821 aaacgctgtc ggtggcgatt ggcgataaag taaaaaaaga ccagctttta ggggtcattg
    11881 atcctgaaca ggctgaaaac cagatcaagg aggtcgaagc aacgctgatg gagctacgcg
    11941 cgcagcggca gcaggcggaa gcggagctga aactggcgcg ggtgacgtat tcccgtcagc
    12001 aacgtctggc acaaacgcag gcggtttcac agcaggatct cgacaccgcc gcgacggaga
    12061 tggctgtgaa acaggcgcaa attggcacca ttgacgcgca aatcaagcgc aatcaggctt
    12121 ctctcgatac ggctaaaacc aatctcgatt acactcgcat cgttgccccg atggccgggg
    12181 aagtcacgca aatcaccact ctgcaaggcc agacggtgat tgccgcacaa caagcaccga
    12241 acattctgac gctggcagat atgagcacca tgctggtaaa agcgcaggtt tctgaagcgg
    12301 atgtaatcca cctgaagccg gggcaaaaag cctggtttac ggtacttggc gatccactga
    12361 cgcgctacga ggggcaaatc aaggatgtac taccgacgcc ggaaaaggtt aacgacgcta
    12421 ttttctatta cgcccgtttt gaagtcccca accccaatgg tttgctgcgg ctggatatga
    12481 ctgcgcaagt gcatattcag ctcaccgatg tgaaaaatgt gctgacgatc cctctgtcgg
    12541 cgttaggcga tccggttggc gataatcgtt ataaagtcaa attgttgcgt aatggtgaaa
    12601 cacgcgagcg tgaagtgacg attggcgcac gtaacgatac cgatgttgag attgtcaaag
    12661 ggctggaagc gggcgatgaa gtggtgattg gtgaggccaa accaggagct gcacaatgac
    12721 gcctttgctc gaattaaagg atattcgtcg cagctatcct gccggtgatg agcaggttga
    12781 ggtgctgaag ggcatcaccc tcgatattta tgccggtgag atggtagcga ttgttggcgc
    12841 ttcaggttcc ggtaaatcga ccctgatgaa tattctcggc tgtctggata aggccaccag
    12901 cggcacctat cgcgtcgccg gtcaggatgt tgccacactg gacgccgatg cgctggcgca
    12961 actgcgccgc gagcatttcg gctttatttt ccagcgttac catttacttt cgcatttaac
    13021 cgccgagcag aacgttgaag tacccgccgt ctatgccggt cttgagcgga aacagcggct
    13081 gcttcgcgcc caggggttac tgcaacgact ggggctggaa gaccgtacag agtattatcc
    13141 ggcacagctt tcgggtggtc agcaacagcg cgtcagcatc gcgcgggcat tgatgaacgg
    13201 cggtcaggta attcttgccg atgaaccaac cggcgcactg gacagccatt ctggcgaaga
    13261 ggtgatggcg atcctgcatc agctgcgcga tcgcgggcat acggtgatta tcgtcaccca
    13321 cgatccgcag gtcgctgctc aggccgagcg ggtgatcgaa attcgcgacg gcgaaattgt
    13381 gcgcaatcct cccgccattg aaaaagtgaa tgttgctggc gggacggagc ctgttgtcaa
    13441 cacggtgtct ggctggcggc agtttgtcag cggttttaac gaggcgctga cgatggcatg
    13501 gcgggcgctg gcagcgaata aaatgcgtac tttactgacc atgctgggga ttattatcgg
    13561 tattgcgtcg gtggtttcca ttgtcgtggt gggtgacgcc gccaaacaaa tggtgctggc
    13621 ggatattcgt tctataggta cgaatactat tgatgtctat cccgggaaag attttggcga
    13681 tgacgatccg caatatcagc aggcgctgaa gtacgacgac ttaatcgcca tccaaaaaca
    13741 accgtgggtc gcctcagcca cacctgccgt ctcgcaaaac ctgcgcctgc gttataacaa
    13801 tgttgatgtt gctgccagtg ccaatggcgt gagcggcgat tattttaatg tctatggcat
    13861 gaccttcagt gaaggaaaca cctttaatca ggagcagctg aacggtcgtg cgcaggtcgt
    13921 ggttctcgac agtaatactc gccgccagct tttcccccat aaagcagatg tggttggcga
    13981 ggtgattctg gtcggcaata tgcccgccag agtcattggt gtggcggaag aaaaacagtc
    14041 gatgtttggt agcagtaaag tgctgcgtgt ctggctacct tacagcacga tgtccgggcg
    14101 agttatgggc cagtcgtggc ttaactccat tactgtcagg gtgaaagaag gatttgacag
    14161 cgccgaggcg gaacagcaac tcacgcgttt actttcactg cgccacggta agaaggattt
    14221 ctttacctgg aacatggacg gcgtcttgaa aactgttgaa aagaccacac gtactttaca
    14281 actgtttctg acgctggtgg cggtgatttc gctggtggtg ggtggtattg gtgtaatgaa
    14341 tattatgctg gtgtcagtga ccgagcggac gcgggaaatt ggcattcgca tggctgtagg
    14401 tgcgcgagca agcgatgttt tgcaacagtt cctgatcgaa gccgtactgg tttgcctggt
    14461 cggtggcgcg ttgggaataa cactgtcact gttaattgct ttcaccttgc agcttttctt
    14521 acccggctgg gagattggtt tttcaccgtt ggcactgctg ctggcgtttc tctgctcgac
    14581 ggccaccggg attttatttg gctggttacc cgcacgaaat gcggcacgac tggatccagt
    14641 agatgctctg gcacgagagt aatttttgag ataaaaatgc cagccgatcg ggctggcatt
    14701 ttgcctttag gatgtacaca atgagacaga agagctatgc gactgccgct tctacttcga
    14761 cgggcacaat aacactggcg tgattgcctt ttggcccctg gtggacatca aactgaacgg
    14821 attgtccagc ttttagcgtt ctgtaaccat ccatctgaat ggtggaataa tgagcgaaaa
    14881 tatcttcgcc gccgccttca gggcagatga aaccaaaccc tttggcattg ttgaaccact
    14941 taacagtacc cttttccatg cttcgacatc cttcgcaaat cttatacaag taagatggaa
    15001 taaaccgggg tcagagaggg ggctgttcaa aacctcgcca actctagaaa tacaatttag
    15061 agaattaggg cgagccgtca agcatttgac aggggacagg gggcaggtat gaatcaaaaa
    15121 tttgaagcag ttaacgctat tgacaggaat gtgacagatg tcgctgatgc caacgataga
    15181 tgatagttat ctatcatgtg gagtagattg gtcaggcaaa taagctcttg tcagcggcag
    15241 ggcgttctgc cgataaccgt aaccgaagat gataactgac aatgggtaaa acgaacgact
    15301 ggctggactt tgatcaactg gcggaagaaa aagttcgcga cgcgctaaaa ccgccatcta
    15361 tgtataaagt gatattagtc aatgatgatt acactccgat ggagtttgtt attgacgtgt
    15421 tacaaaaatt cttttcttat gatgtagaac gtgcaacgca attgatgctc gctgttcact
    15481 accaggggaa ggccatttgc ggagtcttta ccgccgaggt tgcagaaacc aaagtggcga
    15541 tggtgaacaa gtacgcgagg gagaatgagc atccattgct gtgtacgcta gaaaaagcct
    15601 gaatgcaggc ataaaaattg ggggaggtgc ctatgctcaa tcaagaactg gaactcagtt
    15661 taaatatggc tttcgccaga gcgcgcgagc accgtcatga gtttatgacc gtcgagcact
    15721 tgttactggc gctgctcagt aacccatctg cccgggaggc gctggaagcg tgttctgtgg
    15781 atttggttgc gctccgtcag gaactggaag cctttattga acaaaccaca cccgttctgc
    15841 ctgccagtga agaggagcgc gacacacagc cgacgctgag ttttcagcgt gtactgcaac
    15901 gtgcggtctt ccatgtccag tcctccggtc gcaatgaggt aaccggtgca aacgttctgg
    15961 tcgctatctt tagcgaacag gagtcgcagg cggcatatct gttgcgtaaa catgaagtca
    16021 gccgtctcga tgtggtgaac tttatctctc atggcacgcg taaagacgag ccgacacagt
    16081 cttctgatcc tggcagccag ccaaacagcg aagaacaagc tggtggggag gaacgtatgg
    16141 agaatttcac gacgaacctg aatcagcttg cgcgcgtggg cggaatcgac ccactgattg
    16201 gtcgtgagaa ggagctggag cgtgctattc aggttctctg ccgtcgccgt aaaaacaacc
    16261 cgctgctggt gggggaatct ggtgtcggta aaaccgcgat tgcggaaggt cttgcctggc
    16321 gaattgttca gggcgatgtg ccggaagtga tggctgactg tacgatttac tctctcgata
    16381 tcggttctct gttagcgggc actaaatatc gcggcgactt tgaaaaacgt tttaaagcgt
    16441 tgctcaagca gctggagcag gacactaaca gcatcctgtt tattgatgag atccacacca
    16501 ttatcggtgc gggtgcagcg tctggtggcc aggtcgatgc ggctaacctg atcaaaccgt
    16561 tgctctccag cggtaaaatt cgcgtaattg gttcgacaac ctatcaggag ttcagcaaca
    16621 ttttcgagaa agaccgtgct ctggcgcgtc gcttccagaa aattgatatt actgaaccgt
    16681 cgatcgaaga aactgttcaa atcatcaatg gcctgaaacc gaagtatgaa gcgcaccacg
    16741 acgtgcgtta taccgcaaaa gcggtgcgtg cagcggtaga gctggcggtg aaatacatta
    16801 acgatcgtca tctgccggat aaagccattg acgttatcga cgaagcgggc gctcgcgcac
    16861 gcctgatgcc ggtaagcaaa cgcaagaaaa ccgttaatgt ggcggatatt gagtccgtgg
    16921 tggcccgtat tgcgcgcatt ccagagaaga gtgtttctca gagtgaccgc gataccctga
    16981 aaaacctcgg cgatcgcctg aaaatgctgg tcttcggtca ggataaagcc attgaggcgc
    17041 tgactgaagc cattaagatg gcgcgtgcag gtttaggtca cgaacataaa ccggttggtt
    17101 cgttcctgtt tgccggccct accggggtcg ggaaaacaga ggtgacggta cagctttcga
    17161 aagcgttggg cattgagctg ctgcgctttg atatgtccga gtatatggaa cgccataccg
    17221 tcagccgtct gattggtgcg cctccgggat acgttggttt tgatcaggga ggtttgctga
    17281 ctgatgcggt catcaagcat ccacatgcgg tgctgttgct ggacgaaatc gagaaagcgc
    17341 atccggacgt gttcaatatt ctgttgcagg tgatggacaa cggtacgctg accgataaca
    17401 acggacgcaa agcggacttc cgtaacgtgg tgctggtgat gaccaccaac gccggggtac
    17461 gtgaaactga gcgtaaatcc attggtctta tccaccagga taacagcacc gatgcgatgg
    17521 aagagatcaa gaagatcttt acaccggaat tccgtaaccg tctcgacaac attatctggt
    17581 tcgatcatct gtcaactgac gtgatccatc aggtggtgga taaattcatc gtcgagttgc
    17641 aggttcagct ggatcagaaa ggtgtttctc ttgaagtgag ccaggaagcg cgtaactggc
    17701 tggccgagaa aggttacgac cgggcaatgg gcgcacgtcc gatggcgcgt gtcatccagg
    17761 acaacctgaa aaaaccgctc gccaacgaac tgctgtttgg ttcgctggtg gacggcggtc
    17821 aggtcaccgt cgcgctggat aaagagaaaa atgagctgac ttacggattc cagagtgcac
    17881 aaaagcacaa ggcggaagca gcgcattaat ctgattgtca ggtaggttgg tgaagtccgt
    17941 aatctcgtca ggggttacgg actttttatt tatggggtgg aggaggttca gacccttttt
    18001 ttaatgatga tggtaagttg ttgataatta gtgctgcggg aaggtaagga taaaaaaggg
    18061 tgctgcagga gaatgggatg gttttgcttt attaacaacg ggctaaacgt gtagtatttg
    18121 agttcactgc cgtacaggca gcttagaaat cgacggggtg cggtaaaacc tttgcgaacg
    18181 cgttcactgc cgtacagaca gataaaatgc gaaaaaaaag ctcgcacttt cgtacgagct
    18241 cttctttaaa tatggcggtg agggggggat tcgaaccccc gatacgttgc cgtatacaca
    18301 ctttccaggc gtgctccttc agccactcgg acacctcacc aaattgtttt gctaccaaac
    18361 ctcatgggtg gcaacggggc gctactatag ggagttggag taaaacggtc aagaagaatt
    18421 ttaatgataa ttattgtttg ctcatactgt aaacaagttg tgcagtatat ctacatcgag
    18481 acaagttacg gacttatact tccaaagtac ttcatacata tcacaaaata aaaaggccgg
    18541 ttaaaccgac cttttactcg ttctttctct tcgcccatca ggcggtaaaa caatcagcga
    18601 ctacggaaga caatgcggcc tttgctcagg tcgtacgggg tcagttcaac agtcactttg
    18661 tcgcccgtca ggatgcggat gtagtttttg cgcattttac cggagatgtg tgcagtaacc
    18721 acgtgaccgt tttctaactc tacgcggaac atggtattag gcaacgtttc aagaacggta
    18781 ccttgcattt caatattgtc ttctttggcc atctaatcct ctggggtatc actaccgtaa
    18841 tttgaaccgg caagataatg ccgaagttct gtaaataagt aaagatttgc gcgctaaatc
    18901 gcaacaaaca ggttcggcac attactccga aaacacacgg ctaagccgca ccaaaagcgc
    18961 aacgtataag ggagcggtga gataaacgat gggcgttacc tgacgcgaaa aattccttat
    19021 cggcagcggg gtaatgagcg taaccaactc tgcgaccgca attataacac tctggggaga
    19081 aatgtgccga aaacattcat tcttgtggtg aaaacaagca tcgtggtacc cagaaattat
    19141 tcggcaatcg tccgaggcgc atttgattga gataattaag gtaatcccgg cggggaattt
    19201 cgcaggcacc aagcgatgct gtgtgatcgt taaggacctg gcagtcgata agcttaccgc
    19261 catgaccgat aaattcctca cagaatacca gaagcgccgt tttagacgca ttttccatcc
    19321 ggctgaacat ggactcgcca caaaatagcg ttccctgggc cacgccgtac ataccgccga
    19381 caagctcatc ttcacgccag acttcaatgg agtgggcatg gccgagttcg tgaaggcgat
    19441 ggtaggcttc gaccacgcca cgcgtgatcc aggttccttc ttcgcgatcg ctggcacagc
    19501 cttcaatgac ctgaccaaaa gcgtaattca tcgtgacacg atagggcgag cgtttatgaa
    19561 atcgcttcat actacggctg atatgcagtg attctggcca tagcaccgcg cggggatcgg
    19621 gcgaccacca gaggatgggg tcgcctggag aaaaccacgg aaaaatacca cgctggtaag
    19681 ccattaacag gcgcgcaggg ctaagatcgc ccccaagtgc cagcaggccg ttaggctcac
    19741 gtaatgcgcc ttccggggaa gggaaggcta ttgaatggcg agaaagctga accaggcgca
    19801 tgaccgcaaa actccacgca agtcggatcg ttcaataata gcttacaaac cctgcttaaa
    19861 ctggtaataa cgcccctgtc tggcaagcag ttctgcgtga gtaccttgct caataatttg
    19921 cccgttgtcc atcactatta tttgttggaa acgagagagt ccgcgaagtc gatgggtgac
    19981 cattaacacc gttttctcat gcatcatttc tgcaagcaat tcaaggatct gactttcggt
    20041 tgtggcatct aagccttcgg ttggttcatc cagcaatact aacggcgcat cgtgcaatag
    20101 cgcacgggcg atagccagac gacgcagttc gccgccggaa agctgacgtc cgccttctcc
    20161 caaccaactg ttgagccctg tgtcttcgag tagtttctcc agaccgacgc gacggagaat
    20221 ttcagcaaga gtctcatcac tactgccagg cgaggcgagt aacagattat cacgcagcgt
    20281 ggcgctaaac agatgcactc gctgaggaac aacgctgatg gtctgtcgta gagccgcttc
    20341 attcaggctg gctatggggc tatcgttaag caaaatctcg ccctgttgcg ggtcccatgc
    20401 gcgggtcagc agttgtaaca gtgttgattt gccgcatccg gttcgcccga gaatcgcaat
    20461 atgttccccg gcgtttacct gaagagaaat ccctttaagt gcctgttggg attgctccgg
    20521 ataagtgaac tgaacatccc gtaacgtcag cgaaacgcga ttggcaacat gagtttgggt
    20581 atcagggaag gtgacttccg gtttttgatc cgttaagtca gtgatccgaa cggcagaggc
    20641 aatgacttgc cccagatgct gaaatgcacc cgttactggt gccagtgctt caaacgcggc
    20701 taacgcgcag aagacaaaca gggcaattaa cgcgccgggt tgagcattgc cgccaacgcc
    20761 gccagacgcc atccacagca tcaggatcac cgctaacgcg ccaatgagca gcattatcgc
    20821 ttgcgacaat gcggtcagtt cagattgacg gcgttgcgct tccagccatt gaatttctgt
    20881 attctctaat tgcgtgcgat aacgatcgct ggcaccaaaa atggtcagct cagcttgccc
    20941 ttgcagccag gccgtcagtt gttggcgata ctgtccgcga agatgagtca gattttgccc
    21001 ggtgcttttt cccgcacgat aaaacagcgg tggcatcagg aaaagcgtca gtaacataat
    21061 gccgcccagc gtaaaggcga gggtgaaatc aaggaaactt aacccgattg tcaccaccat
    21121 aatcaccaca aaagcgccca ccagcggcga gataacgcgc aggtaaagat gatcgagcgt
    21181 atcaacatcc gccaccacgc ggttgagcaa ttcgccctga cgatagcgcg ccagtccggc
    21241 aggggagagg ggcaacaatt tgctgaaggt gtaaatgcgc agatgctgca acacgcggaa
    21301 agtcgcgtcg tgacttacca gacgttcaaa ataacgcccg gcagtacggg tgattgcggc
    21361 accacgcacg cccgcagcgg gtagcatata gttgaagctg tacagtccgg caacccccgc
    21421 aaccgctgag gccgagagga accagccgga aagtgtcaac agaccgatac tggcgagcag
    21481 cgtgacaatt gccagcacga taccaagact taacatccat ttatgacgtt tatacagtgc
    21541 cagatagggt agcaaagcgc gcatttaaat ctcctcctga cgatgggcca gtaatgtggc
    21601 gaatgggcca ccagccacac ttaattccgc gtaacgtcct tgctcaataa tccggccatc
    21661 ctgcataacc caaatgacat cccagtcagc aagatcttct aactggtggg tgaccattaa
    21721 cgttgtctgg cgcagagagg cggcattcag cgcctccatt acgcgctgtt cactgtgagc
    21781 atcaaggctg gcagcgggtt catccaacag taatagcgaa cagggattta gtaacgcacg
    21841 ggccaccgcc acgcgctgcg cctgccccac ggaaaggcgg gcagcctggt cgccaacagg
    21901 cgtatcaacg ccttgtggca ggagcggtag aaactcgctg acccaggcgt tatccagcgc
    21961 tgcttgtaat tcttgttcgc tggcatcagg tcgcgccagt agtacgttat cccgcaatgt
    22021 tgctgccggt aattgtgggt tttgcccaac ccaggagaga tgtttacgcc atgattctgg
    22081 tgataaatcg cgtaattcta tcccgttgat tcgtaatgat ccctgatatg agagaaaacc
    22141 agaaagcgcg ttcagcagtg agcttttacc tgaaccgctg cgaccaacca acactgcacg
    22201 ttgacctgct ggcaaagtaa agttcagcgg tccggccagc gttttacctt ccggcgacgt
    22261 gataaacaga tcctcggcct caatggtcac cggatcggtt gatgctattt ccgtctcacc
    22321 gcgctgcgga tgggcgagcg gggtttccat aaacgttttc agactgtcag ctgcaccaac
    22381 agcctgggct ttagcatgat aaaacgtacc gagatcgcgt aatggctgga aaaactctgg
    22441 cgcaaggatc agggccagaa aacccgcagc cagcgtcacg ccagtatcgt agtgaccaaa
    22501 atccagctcg ccgagatagg aaaaaccaaa gtagaccgcc accagagcaa ttgacagcga
    22561 ggtaaaaaat tcgagaatgc cggaggataa aaacgccagc cgtagcactt ccattgtccg
    22621 ttggcggaaa tcttccgaag cagaacgaat actttcaatt tcagcttcac cacgaccaaa
    22681 aatacgcaat gtttccatgc cgcgcaggcg atcgaggaaa tgcccactta agcgagcaag
    22741 agcgagaaag ttacgccggt tagcatcggc agcccccatc ccaaccagcg ccataaacaa
    22801 cggaattaac ggtgctgtgc ccagcagaat gagcgccgca gcccagttag aggggaagat
    22861 cgccaccaca atcagcaacg gcaccgacac tgccagcgcc atttgcggca ggtagcgtgc
    22921 atagtaatca tgcatatcgt caatttgctc gagtaccaac gtcgcccagc tccccgcagg
    22981 tttaccctga atccacgctg gccctgcttg ttgcagacgg tcgagaacct gacggcggat
    23041 ggcaaagcgg atatgctgcc cggcgtgata acccacccgt tcgcgtaacc agaccaccca
    23101 tgcgcgcagt acaaaggtca gaaccagtaa cgtaaaggga agcagcaggg cttcacgggg
    23161 aatattctcc ataatcatat gttgcagaat tcgcgccatg aaccaggcct gggcaatgat
    23221 caatatgccg cttacaaagc ccagcagacg agaaatattc agccaacgtt gggagatgac
    23281 gctttgctgt tttaaccagc gggttaactc tttttgacgg gatttattca ttgcacgctt
    23341 agcaggtgag ttatcagaat tatttgcaga gcaatgttac aacggggaaa aaataaaggc
    23401 gacccatagt cgcatggtgt cgccttcttt acttttgtta ctgatttgta aaattatttt
    23461 gcgtcagcta aaccatcgag gtagcgttcc gcatcaagtg ctgccatgca gcctgtaccg
    23521 gccgaagtaa tggcctgacg ataaatatga tccatcacgt cacccgcggc aaagacgcca
    23581 gggatgctgg tctgggtggc attaccatga atacccgact gcactttgat gtagccgttt
    23641 tccagttcca gctgcccttc gaaaatggca gtattcgggc tgtgaccgat agcaacaaac
    23701 agaccagcaa cgtcgagtga ctcgatgtta tcgctgtttt gcgtatcgcg cagacgaacg
    23761 ccagtgacac ccatttgatc gccggtcact tcttccagcg tacggttggt gtgcagaatg
    23821 atgttgccgt tctccacttt atccatcaga cgcttaataa ggattttttc cgcgcggaaa
    23881 ccgtcacggc ggtgaatcag atgcacttcc gaagcgatgt tagacagata cagcgcctct
    23941 tcaaccgcgg tattgccgcc gccgatgacc gcaactttct ggttgcgata gaagaaaccg
    24001 tcgcaggttg cacaagcaga aaccccacgg cctttaaagg cttcttcaga gggcaggccg
    24061 agatagcgtg cagaagctcc ggtggcaata atcagcgcgt cgcaagtgta ttcgccgtta
    24121 tcgccattca gacggaacgg acggttttgc agatccacct tgttgatatg atcaaaaatg
    24181 atctcagttt caaacttggt ggcatgttcg tgcatgcgct ccattaataa cggaccggtc
    24241 agatcgtttg gatcgccagg ccagttttcc acttccgtgg tggtggtcag ttggccgcct
    24301 ttttccatgc cggtaatcag cacaggttgc aggttggcgc gcgccgcgta gacagcagcg
    24361 gtgtatcccg ccgggcctga acccaggata agcagtttac tgtgtttggt cgtgcccatg
    24421 agatccccat agttgttggc agacaatggg caggattgta gggaatttac agacgtaaaa
    24481 aaagagtatg acgattttgt taacaatttg tgcaatcggc agcatcgata agcaggtcaa
    24541 attctcccgt cattatcacc tctgctactt aaatttcccg ctttataagc cgattaaatg
    24601 atgaataaac gcccctgtta atgaatatct ggcatgttgt actaaaaatc gatgttttgc
    24661 tttgacaatc ccctggtgtt ttgcgaaaac attcgaggaa gaaaaaaaac agtattctta
    24721 tatgcgcata accatgcatg taaataccat gtttaccgtg ctagtgaaat ctacgtatgg
    24781 cgtggacaga cgccattcgt gatgtcgata gctgccacaa ggcaacggtc ttctcaccgt
    24841 agacccaggc attgcgcgcc gtgaatcttc atgatttcgg tctatcgtga cgggtagcga
    24901 ctctgaacag tgatgtttca gggtcagaca ggagtaggga aggaatacag agagacaata
    24961 ataatggtag atagcaagaa gcgccctggc aaagatctcg accgtatcga tcgtaacatt
    25021 cttaatgagt tgcaaaagga tgggcgtatt tctaacgtcg agctttctaa acgtgtggga
    25081 ctttccccaa cgccgtgcct tgagcgtgtg cgtcggctgg aaagacaagg gtttattcag
    25141 ggctatacgg cgctgcttaa cccccattat ctggatgcat cacttctggt attcgttgag
    25201 attactctga atcgtggcgc accggatgtg tttgaacaat tcaataccgc tgtacaaaaa
    25261 cttgaagaaa ttcaggagtg tcatttagta tctggtgatt tcgactacct gttgaaaaca
    25321 cgcgtgccgg atatgtcagc ttaccgtaag ttgctggggg aaaccctgct gcgtctgcct
    25381 ggcgtcaatg acacacggac atacgttgtt atggaagaag tcaagcagag taatcgtctg
    25441 gttattaaga cgcgctaaca cggaacaggt gcaaaatcgg cgtattttga ttacactcct
    25501 gttaatccat acagcaacag tactggggta acctggtact gttgtccgtt ttagcatcgg
    25561 gcaggaaaag cctgtaacct ggagagcctt tcttgagcca ggaatacact gaagacaaag
    25621 aagtcacatt gacaaagtta agtagcggcc gtcgccttct ggaagcgttg ctgatcctta
    25681 ttgtcctgtt tgccgtctgg ttgatggctg ccttactaag ctttaaccct tcggacccca
    25741 gctggtcgca aacggcctgg catgaaccta tccataattt aggtgggatg cccggtgcgt
    25801 ggctggcaga tacgctgttc tttatttttg gcgtgatggc ttacaccatt cccgtcatta
    25861 ttgtcggcgg ttgttggttt gcctggcgtc atcagtccag tgacgaatac attgattatt
    25921 ttgccgtttc gctacgcatc attggcgttt tggcgcttat ccttacctcc tgtggtctgg
    25981 cggcaatcaa cgctgacgat atctggtatt ttgcctccgg tggcgtcatt ggcagcttac
    26041 taagcactac gctacaacca ctgctacaca gtagcggggg aactattgcg ctgctctgcg
    26101 tttgggctgc gggcctgacg ctgttcaccg gttggtcatg ggtgaccatt gctgaaaaac
    26161 tcggcggctg gattttaaac attctcacct tcgccagtaa tcgtacccgt cgcgatgata
    26221 cctgggtcga tgaagatgaa tatgaagatg acgaagagta tgaagatgaa aaccacggca
    26281 aacagcatga atcacgccgt gcccgtattc ttcgcggcgc gctagcgcgt cgtaaacggt
    26341 tggcggaaaa attcattaat ccgatggggc ggcaaacaga cgctgcgttg ttctccggta
    26401 agcggatgga tgatgacgaa gagattatct acactgcacg cggtgtggct gccgacccgg
    26461 acgacgtcct attttcgggc aatcgtgcaa cgcagccaga atatgacgaa tacgatccat
    26521 tattaaacgg tgcgccaatt accgaacctg tcgctgtagc agctgctgct accacggcga
    26581 cacaaagctg ggctgcgccg gttgaacctg tgactcagac gccgcctgtt gcctctgttg
    26641 atgttccacc ttcgcaacct acagtagcct ggcagcctgt accgggtcca caaacgggag
    26701 agccggttat tgctcctgca ccggaaggtt acccacagca gtcacaatat gcgcagcctg
    26761 cagtgcaata taatgagccg ctgcaacaac cagtacagcc gcagcagccg tattatgcac
    26821 ctgcagctga acaacctgcg caacaacctt actacgctcc tgcggcggaa caacctgttc
    26881 agcaaccgta ttacgcccct gcgccagaac aaccggtggc aggtaacgcc tggcaagccg
    26941 aagagcagca atccactttt gctccacagt ctacatacca gactgagcaa acttatcagc
    27001 aaccagccgc tcaggagccg ttgtaccaac agccgcaatc cgttgaacag cagcctgttg
    27061 tggagcctga acccgttgta gaagagacaa aacccgcgcg tccgccgctt tactattttg
    27121 aagaagtgga agagaagcga gcccgtgaac gtgaacaact tgcggcctgg tatcaaccga
    27181 ttccagaacc ggttaaagaa ccagaaccga tcaaatcttc gttgaaagcg ccttctgttg
    27241 cagcagtacc tccagtagaa gccgctgccg ctgtttctcc gctggcatct ggcgtgaaaa
    27301 aagcgacact ggcgacgggg gctgccgcaa ccgttgccgc gccagtcttc agtctggcaa
    27361 atagcggtgg accgcgtccg caagtcaaag aggggattgg cccgcagttg ccacgaccga
    27421 aacgtatccg cgtgccaact cgtcgtgaac tggcgtctta cggtattaag ctgccctcac
    27481 agcgtgcggc ggaagagaaa gcccgtgaag cccagcgcaa tcagtatgat tctggcgatc
    27541 agtataacga tgatgaaatc gatgcgatgc agcaggatga actggcacgt cagttcgccc
    27601 agacacagca gcaacgctat ggcgaacagt atcaacatga tgtgcccgta aacgcagaag
    27661 atgcagatgc tgcggcagag gctgaactgg ctcgtcagtt tgcgcaaact caacaacaac
    27721 gttattccgg cgaacaaccg gctggggcga atccgttctc gctggatgat tttgaatttt
    27781 cgccaatgaa agcgttgctg gatgatggtc cacacgaacc gttgtttacg ccaattgttg
    27841 aacctgtaca gcagccgcaa caaccggttg caccgcagca gcagtatcag cagccgcaac
    27901 aaccagttcc gccacagcag caatatcagc agccgcaaca gccggttgcg ccgcagccgc
    27961 agtatcagca gccacaacag caggttgcgc cgcagccaca atatcagcag ccgcaacaac
    28021 cggttgcgcc acagccgcaa tatcagcagc cgcaacaacc agttgcgccg cagccgcagt
    28081 atcaacagcc gcaacaaccg gttgcaccgc agcagcagga taccctgctt catccgctgt
    28141 tgatgcgtaa tggcgacagc cgtccgttgc ataaaccgac gacgccgctg ccttctctgg
    28201 atttgctgac accgccgccg agcgaagtgg agccggtaga tacctttgcg cttgaacaaa
    28261 tggctcgcct ggtggaagcg cgtctggctg atttccgtat taaagccgat gtcgtcaatt
    28321 actctccggg gccggttatc actcgctttg aacttaacct ggctcctggc gtaaaagcgg
    28381 cgcgcatttc taacttgtca cgggaccttg cccgttcact ttcgacggtg gcggtgcgtg
    28441 tcgttgaagt tattcctggc aaaccctatg taggtctgga gttaccaaat aaaaaacgac
    28501 aaaccgttta tctgcgcgaa gttttggata acgccaaatt ccgcgataat ccgtcgccat
    28561 taaccgtggt gctgggtaaa gatatcgccg gtgagccggt ggttgccgat ctggcgaaaa
    28621 tgccgcactt gttggttgcg gggactaccg gttccggtaa atctgtcggt gtgaacgcga
    28681 tgatcctgag catgctttat aaagcacagc cagaagatgt gcgtttcatc atgatcgacc
    28741 cgaaaatgct ggagctttcg gtttatgaag gcattccgca tctgttaacg gaagtcgtta
    28801 ctgatatgaa agatgccgcc aacgcgctgc gctggtgtgt taacgagatg gagcgtcggt
    28861 ataaactgat gtctgcgctg ggtgtgcgta atctggcggg ttataacgaa aaaattgctg
    28921 aagccgatcg catgatgcgt ccgattccag acccgtactg gaagccgggt gacagtatgg
    28981 atgcccagca tccggtgctg aaaaaagaac catacattgt ggtgttggtt gacgaatttg
    29041 ccgacctgat gatgacggta ggtaaaaaag tggaagagct gatagcacgt ctggcgcaaa
    29101 aagcccgtgc cgcgggtatc cacctcgtac tggcaactca gcgtccatcg gttgatgtta
    29161 ttactggtct gattaaagcg aatattccga cccgtatcgc ctttaccgta tccagtaaga
    29221 ttgactcacg taccattctt gatcaggctg gcgcggaatc actgctgggg atgggggata
    29281 tgctctactc tgggccgaac tccacgttgc cggtacgtgt ccatggtgct tttgttcgcg
    29341 atcaggaagt tcatgccgtg gtgcaggact ggaaagcgcg tggtcgccca cagtatgttg
    29401 atggcatcac ttccgacagt gaaagcgaag gtggcgcggg cggtttcgat ggcgctgaag
    29461 aactggatcc gttgttcgat caggcggtgc agtttgtcac tgaaaaacgc aaagcgtcaa
    29521 tttctggcgt acagcgtcag ttccgcattg gttataaccg tgcagcgcgt attatcgaac
    29581 agatggaagc gcaggggatt gtcagcgaac aggggcacaa cggtaatcgt gaagtgctgg
    29641 ccccaccgcc gtttgactaa ttaatgcatt gccggataag gcgcggtagc gtcgcatccg
    29701 gcactctatc aactgaaaat tcagtatttt cttctttcct caagctgatt attagcctgg
    29761 aatagagagt agagggaact cccgatcggg agtgacgtaa tttgaggaat aatgatgaaa
    29821 aaaattgcca tcacctgtgc attactctca agcttagtag caagcagcgt ttgggctgat
    29881 gccgcaagcg atctgaaaag ccgcctggat aaagtcagca gcttccacgc cagcttcaca
    29941 caaaaagtga ctgacggtag cggcgcggcg gtgcaggaag gtcagggcga tctgtgggtg
    30001 aaacgtccaa acttattcaa ctggcatatg acacaacctg atgaaagcat tctggtttct
    30061 gacggtaaaa cactgtggtt ctataacccg ttcgttgagc aagctacggc aacctggctg
    30121 aaagatgcca ccggtaatac gccgtttatg ctgattgccc gcaaccagtc cagcgactgg
    30181 cagcagtaca atatcaaaca gaatggcgat gactttgtcc tgacgccgaa agccagcaat
    30241 ggcaatctga agcagttcac cattaacgtg ggacgtgatg gcacaatcca tcagtttagc
    30301 gcggtggagc aggacgatca gcgcagcagt tatcaactga aatcccagca aaatggggct
    30361 gtggatgcag cgaaatttac cttcaccccg ccgcaaggcg tcacggtaga tgatcaacgt
    30421 aagtagaggc acctgagtga gcaatctgtc gctcgatttt tcggataata cttttcaacc
    30481 tctggccgcg cgtatgcggc cagaaaattt agcacagtat atcggccagc aacatttgct
    30541 ggctgcgggg aagccgttgc cgcgcgctat cgaagccggg catttacatt ctatgatcct
    30601 ctgggggcca ccgggtaccg gcaaaacaac cctcgctgaa gtgattgccc gctatgcgaa
    30661 cgctgatgtg gaacgtattt ctgccgtcac ctctggcgtg aaagaaattc gcgaggcgat
    30721 cgagcgcgcc cggcaaaacc gcaatgcagg tcgccgcact attctttttg ttgacgaagt
    30781 tcaccgtttc aacaaaagcc agcaggatgc atttctgcca catattgaag acggcaccat
    30841 cacttttatt ggcgcaacca ctgaaaaccc gtcgtttgag cttaattcgg cactgctttc
    30901 ccgtgcccgt gtctatctgt tgaaatccct gagtacagag gatattgagc aagtactaac
    30961 tcaggcgatg gaagacaaaa cgcgtggcta tggtggtcag gatattgttc tgccagatga
    31021 aacacgacgc gccattgctg aactggtgaa tggcgacgcg cgccgggcgt taaatacgct
    31081 ggaaatgatg gcggatatgg ccgaagtcaa tgatagcggt aagcgggtcc tgaagcctga
    31141 attattgacc gaaatcgccg gtgagcgaag cgcccgcttt gataacaaag gcgatcgctt
    31201 ttacgatctg atttccgcac tgcataagtc ggtacgtggt agcgcacccg atgcggcgct
    31261 gtactggtat gcgcgaatta ttaccgccgg tggcgatccg ttatatgtcg cgcgtcgctg
    31321 tctggcgata gcgtctgaag atgtgggtaa tgctgatcca cgagcgatgc aggtggcaat
    31381 tgcggcctgg gattgcttta ctcgcgttgg cccggcggaa ggtgaacgcg ccattgctca
    31441 ggcgattgtt tacctagcct gcgcgccaaa aagcaacgct gtctacactg cgtttaaagc
    31501 cgcgctggcg gatgctcgcg aacgtccgga ttatgacgtg ccggttcatt tgcgtaatgc
    31561 gccgacgaaa ttaatgaagg aaatgggcta cgggcaggaa tatcgttacg ctcatgatga
    31621 agcaaacgct tatgctgccg gtgaggttta cttcccgccg gaaatagcac aaacacgcta
    31681 ttatttcccg acaaacaggg gccttgaagg caagattggc gaaaagctcg cctggctggc
    31741 tgaacaggat caaaatagcc ccataaaacg ctaccgttaa tgttatcgtt gcggtaatgt
    31801 tgttactgta tccctgtggt cgcaggctgt ggccacatct cccatttaat tcgataagca
    31861 caggataagc atgctcgatc ccaatctgct gcgtaatgag ccagacgcag tcgctgaaaa
    31921 actggcacgc cggggcttta agctggatgt agataagctg ggcgctcttg aagagcgtcg
    31981 taaagtattg caggtcaaaa cggaaaacct gcaagcggag cgtaactccc gatcgaaatc
    32041 cattggccag gcgaaagcgc gcggggaaga tatcgagcct ttacgtctgg aagtgaacaa
    32101 actgggcgaa gagctggatg cagcaaaagc cgagctggat gctttacagg ctgaaattcg
    32161 cgatatcgcg ctgaccatcc ctaacctgcc tgcagatgaa gtgccggtag gtaaagacga
    32221 aaatgacaac gtcgaagtca gccgctgggg caccccgcgt gagtttgact ttgaagttcg
    32281 tgaccatgtg acgctgggtg aaatgcactc tggcctcgac tttgcagccg cagttaagct
    32341 gaccggttcc cgctttgtgg taatgaaagg gcagattgct cgcatgcacc gcgcactgtc
    32401 gcagtttatg ctggatctgc ataccgaaca gcatggctac agtgagaact atgttccgta
    32461 cctggttaac caggacacgc tgtacggtac gggtcagctg ccgaaatttg ctggcgatct
    32521 gttccatact cgtccgctgg aagaagaagc agacaccagt aactatgcgc tgatcccaac
    32581 ggcagaagtt ccgctgacca acctggtacg cggtgaaatc attgatgaag atgatctgcc
    32641 aattaagatg accgcccaca ccccatgctt ccgttctgaa gccggttcat atggtcgtga
    32701 cacccgtggt ctgatccgta tgcaccagtt cgacaaagtt gaaatggtgc agatcgtgcg
    32761 cccggaagac tcaatggcgg cgctggaaga gatgactggt catgcagaaa aagtcctgca
    32821 actgctgggc ctaccgtacc gtaaaattat cctttgtacc ggcgacatgg gctttggcgc
    32881 ttgcaaaact tacgacctgg aagtatggat cccggcacag aacacctacc gcgagatctc
    32941 ttcctgctcc aacgtttggg atttccaggc acgtcgtatg caggcacgtt gtcgcagcaa
    33001 gtcggacaag aaaacccgtc tggttcatac cctgaacggt tctggtctgg ctgttggtcg
    33061 tacgctggtt gcggttatgg aaaactatca gcaggctgat ggtcgtattg aagtaccaga
    33121 agttctgcgt ccgtatatga acggactgga atatattggc taatacccaa tttttctgaa
    33181 tctaaaaagc gcctgcgggc gctttttttg tctccctttg ataccgaaca ataattactc
    33241 ctcacttaca cgtaatacta ctttcgagtg aaaatctacc tatctctttg attttcaaat
    33301 tattcgatgt atacaagcct atatagcgaa ctgctataga aataattaca caatacggtt
    33361 tgttactgga atcaatcgtg agcaagcttg agtgagccat tatgaaaacg aaaatccctg
    33421 atgcggtatt ggctgctgag gtgagtcgcc gtggtttggt aaaaacgaca gcgatcggcg
    33481 gcctggcaat ggccagcagc gcattaacat taccttttag tcggattgcg cacgctgtcg
    33541 atagcgccat tccaacaaaa tcagacgaaa aggttatctg gagcgcctgt acagttaact
    33601 gtggtagtcg ctgcccgcta cgtatgcacg tcgtggacgg tgaaatcaaa tatgtcgaaa
    33661 cggacaatac cggcgatgac aattacgacg gcctgcacca ggttcgcgcc tgtctgcgtg
    33721 ggcgttccat gcgtcgccgt gtctacaatc cggaccgcct gaaatatccg atgaaacgag
    33781 tcggggcgcg cggtgaaggc aaattcgagc gcattagctg ggaagaagcc tacgacatca
    33841 tcgcgaccaa tatgcagcgc ctgatcaaag agtacggcaa cgagtctatc tatctgaact
    33901 atggcaccgg tacgctgggc ggcaccatga cccgctcctg gccgccggga aataccctgg
    33961 tcgcgcgcct gatgaactgc tgcggcggtt atcttaacca ttacggtgac tactcttctg
    34021 cgcaaattgc ggaaggtttg aactatacct acggcggctg ggcagatggt aacagcccgt
    34081 cggatatcga aaacagtaaa ttggtagtgc tgtttggtaa taacccaggc gaaacgcgaa
    34141 tgagtggcgg tggggtgact tactatcttg aacaggcacg ccagaaatct aatgcccgca
    34201 tgatcatcat cgatccgcgc tataccgaca ccggtgccgg gcgcgaagat gagtggatcc
    34261 ctattcgtcc aggaacagat gccgcactgg ttaacggtct ggcgtatgtc atgatcaccg
    34321 aaaacctggt ggatcaggca ttccttgata aatattgcgt tggctacgat gagaaaaccc
    34381 tgcccgctag cgcgccgaaa aatggccact ataaagctta tattctgggt gaagggccag
    34441 atggcgtggc taaaacaccg caatgggcct cgcaaatcac cggtatcccg gcagagaaaa
    34501 tcattcaact ggcacgggaa atcggcagta ccaaaccggc gtttatcagc cagggatggg
    34561 ggccacagcg ccacgccaac ggcgaaatcg cgactcgcgc tatctcgatg ctggcgattc
    34621 tgaccggtaa cgtcggtatt aacggaggca acagcggcgc gcgtgaaggt tcatacagct
    34681 taccgtttgt ccgtatgccg accttggaaa acccgatcca gaccagcatt tcgatgttta
    34741 tgtggaccga tgccattgaa cgtggcccgg aaatgacagc gctgcgtgat ggtgtacgcg
    34801 ggaaagataa gctggatgtg ccgatcaaaa tgatctggaa ctatgccggt aactgcctga
    34861 ttaaccagca ttctgaaatc aaccgtaccc atgaaatcct tcaggatgat aagaagtgcg
    34921 agctgattgt ggttatcgac tgccacatga cctcatcggc gaaatatgct gacatcctgc
    34981 tgcctgactg caccgcttcc gaacagatgg actttgcact ggatgcatcc tgcgggaata
    35041 tgtcttacgt gattttcaac gatcaggtga ttaaaccgcg ctttgaatgt aagaccatct
    35101 atgaaatgac cagcgaactg gcaaaacgtc ttggcgttga gcaacagttt actgaaggcc
    35161 gtacacagga agagtggatg cggcatctgt atgcccagtc gcgggaagcg attcctgaac
    35221 tgccaacgtt tgaagagttc cgcaagcagg ggatctttaa aaagcgcgac ccacaagggc
    35281 atcacgttgc ttataaagcc ttccgtgaag atccgcaggc aaacccactg actacgccat
    35341 cgggcaaaat tgagatttat tcgcaggcgc tggctgacat tgccgctacc tgggaattgc
    35401 cagaaggcga tgtgatcgat ccactgccga tctacacgcc gggctttgaa agttatcagg
    35461 atccgctgaa caaacagtat ccgctgcagc ttacgggttt ccactataaa tctcgcgttc
    35521 actcaactta cggcaacgtt gatgtgctga aagcggcttg ccgtcaggaa atgtggatca
    35581 acccgcttga tgcgcaaaaa cgcggtatca acaacggcga taaagtcagg atctttaacg
    35641 atcgtggtga agttcatatt gaggcgaaag tgacgccacg aatgatgccg ggtgtggtcg
    35701 cactgggtga aggtgcctgg tatgacccgg atgcaaaacg tgtcgataag ggcggttgta
    35761 ttaacgtact gaccactcaa cgtccgtctc ctctcgctaa ggggaatccg tcacatacaa
    35821 accttgttca ggttgaaaag gtgtaaggag taaccgatga caacccagta tggatttttt
    35881 attgattcca gccgttgcac cggttgcaaa acctgcgagc tggcctgtaa agactacaaa
    35941 gatttgacgc cagaagtcag cttccgccgc atttatgaat atgctggcgg cgactggcag
    36001 gaagataacg gtgtctggca ccagaacgtg tttgcctact atctgtcgat ttcatgtaac
    36061 cactgcgaag atccggcttg tactaaagtc tgcccgagcg gtgcgatgca taaacgcgaa
    36121 gatggttttg ttgtggtcga tgaagatgta tgcattggct gccgctactg tcatatggct
    36181 tgcccgtatg gcgcaccgca atataacgaa acgaaaggcc atatgaccaa atgcgatggt
    36241 tgttatgacc gtgttgcgga gggtaaaaag ccgatctgtg ttgaatcttg cccgctgcgg
    36301 gcgctggatt tcggtcctat cgacgagctg cgtaaaaaac atggcgatct ggcggccgtt
    36361 gcgccgttgc cgcgagctca ctttaccaaa ccgaatattg tgatcaaacc caatgccaat
    36421 agccgcccga ccggggatac cactggctat ctggcaaacc cgaaggaggt gtgagatggg
    36481 aagtggatgg catgaatggc cgctgatgat cttcacggtc ttcgggcaat gtgtagcagg
    36541 tggttttatc gttctggctt tggcgctgct caaaggcgac ctgcgagcag aagcccagca
    36601 gcgtgttatc gcctgcatgt ttggtttatg ggtgctgatg ggcattggct ttatcgcctc
    36661 tatgctccat cttggttcac caatgcgcgc ttttaactcg ctcaaccggg taggggcttc
    36721 agcactcagt aacgaaatcg ccagcggttc gatctttttt gccgtaggcg gcatcggctg
    36781 gttgctggca atgctgaaaa agctgtcacc ggcattgcgt acgctgtggc tgatagtgac
    36841 gatggttctt ggcgtcatct ttgtctggat gatggtgcgt gtgtataaca gcattgatac
    36901 cgttccgacc tggtacagca tctggacgcc gatgggcttc ttcctgacga tgtttatggg
    36961 cggcccgtta ctgggttacc tgttgttgag tctggctggc gtcgatggct gggcgatgcg
    37021 tctgctgcca gccatttctg tactggcact ggtggtgagt ggcgtgatgt cagtgatgca
    37081 gggcacagag ctggcaacta ttcatagttc tgtgcagcag gccgcagcgc tggtgccgga
    37141 ctatggtgcg ctgatgtcct ggcggatcgt gcttttggcc gttgccctgt gcttgtggat
    37201 tgcaccacag ctaaaaggtt atcagcctgc ggttccgcta ctatccgtct cattcattct
    37261 gcttctggca ggggaattaa tcggtcgcgg cgtattctat ggtttgcata tgaccgtggg
    37321 gatggccgtc gcaagctaat cagtaacaac cggggtttcg gccccggttc tcttttattt
    37381 ctgcttcgtt aacgtgtcat aactggtcat caaattacga taatcaggaa tatggttaga
    37441 gaacaacgtc gccagtcctt caatatcatt acgccagtca cggtgcagct cgcacgccac
    37501 gccgaaccat gtcatcaatt gcgcacctgc ttgcgacatg cgatcccatg ccgaatgccg
    37561 ggtaatttca ttaaaggtcc ctgacgcgtc agtcacaaca aagacatcaa acccttcttc
    37621 aatggctgaa agcgccggga atgccacgca aacttcggtt accacaccgg caataattaa
    37681 ctgtttttta cctgtcgctt tgactgcttt tacaaaatct tcgttatccc aggcgttaat
    37741 atttcccggg cgagcaatgt aaggtgcatc ggggaattgt gctttcagtt ctggaactaa
    37801 tggaccgtta ggaccggttt caaaactggt ggtaagaata gtaggtaagt tgaaatactt
    37861 agccaggtca cccagcgcca gcacgttatt tttaaactta tcgggttcga tatcccgtac
    37921 aagggaaagt aaaccagcct gatgatcaac aagcaaaacg gcagcatcat ttttatcaag
    37981 acgaacatac ggtttggtca tcctcttctc ctttcgagag tcagtggtac gtctgaaatc
    38041 ggtcagacgt gatgcattgc tctgaaagca tagacgggaa atatgagttt gctgtgacca
    38101 tgaaattttt cgactgaacg aagcgtatga aatttgtgtt agatcaataa aaacaatcag
    38161 atgagattaa tcatctcttc agcattagcg ttatgaatga gtaatataaa tcgataatac
    38221 cgccgctgcg atatgcgcgt attttcaccc attgacaatg tttttggcgg tggcatgatg
    38281 cgcatgaaat ttgaacttcc tcacggtttt aattcatgtc cacgtatacc cggcctgtca
    38341 tgcttttgct gtctggcctg cttttgttga ctctggcgat cgcggtgtta aatacactcg
    38401 tgccgctttg gctcgcccag gaacacatgt ccacatggca ggtaggcgtt gtcagctcat
    38461 cctattttac cggcaacctt gtcggtacat tgctgacagg gtatgtcatt aaacgcattg
    38521 gctttaaccg cagctattat ctggcctcct tcatttttgc cgctggctgt gccggccttg
    38581 gcctgatgat tggtttctgg agctggttgg cgtggcgttt tgtcgctggc gtcggctgtg
    38641 ccatgatttg ggtggttgtt gagagcgcgc tgatgtgcag tgggacgtca cgtaaccgtg
    38701 ggcgtttgct tgctgcatac atgatggttt attacgtggg aacgttttta ggccagttac
    38761 tggtcagcaa ggtctcaacc gagctgatga gcgtattgcc gtgggttaca ggtttgacat
    38821 tggcagggat cttaccgctg ttgtttacgc gtgtgctgaa tcagcaggct gaaaaccatg
    38881 attcgacgtc aattacggca atgctaaaac tccgtcaggc gcggcttggc gtgaatggct
    38941 gcattatctc aggaatcgtt ctgggatctc tatatggcct gatgccgttg tacctcaatc
    39001 acaaaggggt gagcaatgcc agtattggtt tctggatggc ggtactggtc agtgcgggta
    39061 tcctcggaca atggccgatt ggacgtctgg cggataagtt tggtcgactg ctggtgttgc
    39121 gtgttcaggt ctttgtcgtc attctcggca gtatcgcgat gcttagccag gcggcgatgg
    39181 cccctgcgtt attcatcctc ggtgccgctg gctttacgct atatccggtg gcgatggcct
    39241 gggcttgtga gaaagttgaa catcatcaac tggtggcgat gaaccaggcc ttactgttga
    39301 gttatactgt gggaagtctg cttggcccgt catttaccgc tatgctaatg cagaatttct
    39361 ccgataattt attgtttatc atgatcgcca gcgtatcgtt tatctatttg ctgatgctgc
    39421 tgcgcaacgc cggtcatacg ccgaaacccg ttgctcacgt gtaaatgaat tcaagcagag
    39481 tgtgaactta ctgtttcaca ctctgctttt ttgtttcttc tatctgactt gctttattcc
    39541 aaattttatt cgtttaaaaa taaaatgtgc agcaggttat aattttgcat ttcgcgattt
    39601 ccgcacttct tatttgccgc gcataatccc tcgttttaca gatgcccctt taattttggc
    39661 gaaggatttg tctatggctg ggaatgttca ggaaaaacag ttgcgatggt acaacattgc
    39721 gctgatgtct tttatcactg tctggggttt tggcaacgtt gttaataact atgccaacca
    39781 ggggctggtg gttgtttttt catgggtgtt tatctttgca ctctatttca caccttatgc
    39841 gctaattgtt ggtcagttag gctcgacctt caaagatggg aagggcgggg tcagtacctg
    39901 gattaaacac acgatgggac ccggactggc ttatctcgcc gcgtggacct actgggtggt
    39961 gcatattccc tatctggcac aaaaacccca ggcaattctg attgcgctcg gttgggcgat
    40021 gaaaggcgac ggttcgctaa tcaaagaata ttcagtcgta gcgttacagg ggttaacgct
    40081 ggtgctgttt atcttcttta tgtgggttgc ttcacgcggt atgaaatcgc tgaaaatcgt
    40141 cggttctgtg gcagggattg cgatgtttgt tatgtcgctc ctgtatgtgg cgatggcggt
    40201 aaccgcgcct gcgattactg aagtgcatat tgcgaccaca aacattacct gggaaacgtt
    40261 cattcctcat atcgacttta cctacattac cactatttca atgctggttt tcgcggttgg
    40321 cggagcagag aagatttctc cttacgttaa tcaaacgcgc aacccaggaa aagaatttcc
    40381 aaaagggatg ctatgcctgg cggtgatggt tgcggtttgt gccattctgg gctcactggc
    40441 gatggggatg atgtttgact cgcgtaatat cccggatgac ttaatgacga acggtcaata
    40501 ttacgccttt cagaagctgg gcgagtatta caacatgggt aatactttaa tggtgattta
    40561 cgccattgcg aataccctgg gacaagtagc tgcgctggta ttctcgattg atgccccgct
    40621 taaagtgcta ttaggtgatg ctgacagcaa atatattcca gccagtttat gtcgtaccaa
    40681 cgcttctggt acgcccgtta atggctattt tctgaccctg gtgctggtgg caattctgat
    40741 tatgctgccg actctcggca tcggtgatat gaacaatctc tataaatggc tgttgaatct
    40801 taactcggta gtgatgccgc tgcgttattt atgggtattt gttgcattta ttgcagtcgt
    40861 tcgcctggcg cagaaatata aaccagaata tgtctttatt cgtaataagc ctctggcaat
    40921 gaccgtcggg atttggtgtt ttgcctttac cgcctttgcc tgtttgacgg ggatcttccc
    40981 gaaaatggaa gccttcactg cagagtggac cttccagttg gcgctgaatg ttgcaacgcc
    41041 gtttgtgctg gtaggattag gactgatttt cccgctgctg gcgcgtaaag cgaatagtaa
    41101 ataattattg tggtcggtga cgctgactct ggcagatctg acccgttttt acataatcga
    41161 gaaatgcgcg caagccgcag gacatatact ggcggtttgg ataatagatc tggaagcctg
    41221 gacgctctgt gctccagtct tccagtacac attctaaacg accagtatca aggtactcct
    41281 tgatctgttc atagagcaga tacccgatac cagcacccat caaaacggcc tccagttcag
    41341 catcaacatc atccagaatg atatttcctg cgacagcaat ttccagctcc tttgcaaatt
    41401 gccagtggaa aggtttgccg ctgggataac gaaaaacaac acattgatga ttaagcagat
    41461 catgaggatg gcggggtttg ccatatcttg caaagtactc tggcgttgct gctacacata
    41521 atttcactgg cgggccgatc gccacgctga tcatatcttt ttcgacgatg ccactaagtc
    41581 gaacgccagc gtcaaagccc tgttggacga tatcgacaag agagtcgtca gttgtgagct
    41641 cgactttaat atcaggatat tcacgtgtga aaccaaccag taaaggcatt aaaaatatac
    41701 gtgcagctac ccgggcggca ttgattttga gcgttcctgt cggtgtcaga cgaaaatcat
    41761 tcatctcatc taacatgatc tgaatttcgt cgaaagctgg gcgcaatcgt tcatagagat
    41821 tagaacccgc ttccgttaac gatacgcttc gggttgtccg attgaataag cgaattttaa
    41881 gacgttgttc cagtgtttta atactatggc taatggcgga cgaggataag cctaattcat
    41941 cgcccgctgc acgaaagctt tgattacggg ccacggcaaa gaaagtggca aagtcagaca
    42001 tattcatccg cattagtgaa aatccttcat taactcatcc tgaatcatca cgtttacagg
    42061 attatgccgc ctgcgtaaag tgcgctccag aacttaacgt ggaggtaaaa ttatgcagtc
    42121 tgaacgtatt tatttggtat gggcccatcc tcgtcatgat tcattgaccg cacatattgc
    42181 tgatgcgatc catcagcggg caatggagcg gaaaatacag gtgacggaac tcgatttata
    42241 tcggcgtaat ttcaacccgg tgatgacgtc ggaagatgaa ccagactgga agaatatgga
    42301 taaacgttat tctccagagg ttcatcagct ttattcagag ctgcttgagc atgacacgtt
    42361 agtggtggtt tttcctctct ggtggtacag cttcccggca atgctaaaag gatatattga
    42421 cagagtatgg aataatgggc tggcttatgg agatgggcac aaattaccat tcaataaagt
    42481 tcgttgggtg gcgctggttg gaggagacaa agaatcattt gtccagatgg gctgggaaaa
    42541 aaatataagc gattatttaa aaaatatgtg cagttatctt ggtattgaag atgccgatgt
    42601 cactttcttg tgtaatacag tggtattcga tggggaagaa cttcacgcga gctattatca
    42661 gtcgttatta tctcaggtac gggatatggt agatgcacta taagatgtgt taaaaacgct
    42721 gtagcagaat gaagcgcgga ataaaaaagc ggcaactcaa taaagttgcc gctttacggg
    42781 gaaattagaa cattacctta tgaccgtact gctcaagaat gcctttcacg cgttccatgg
    42841 tctctttctt cggtggttta acaccgtcga gtttgtactc ttcacccatt gccacccatt
    42901 tgtgtttgcc cagctcgtgg taggggagaa gctcgatttt ctcaacgttg cccatatcac
    42961 gggtaaattc accgaggcga tgcgctgaat cgtcatcgtc agaccagcct gggacaacaa
    43021 cgtagcggat ccacaccttc acatttttgt tcgccagata tttggcgaac tccagcgtgc
    43081 ggtgattgga aactccaacc agattttggt ggatctcgtc gttcatctgt ttgagatcga
    43141 gcattaccag gtcggttact tccagcagtt catcaatcac cggatcgtaa cgacgaacaa
    43201 aaccgttggt gtccagacag gtatgaatgc cttctttttt gcaggcgcgg aaccagtcac
    43261 gaacaaactc agcttgtagg attgcctcac cgccggatgc ggtaacgccg ccgccggaag
    43321 cgttcataaa gtggcgatag gtcaccacct ctttcattaa atcttcaacg gtaacttctt
    43381 taccgccatg cgtatcccag gtgtcgcggt tatgacaata caggcagcgc atcaggcagc
    43441 cctggaaaaa ggtgataaag cgaatacctg ggccgtctac ggttccacag gattcaaagg
    43501 agtgaatgcg accaataact gacattgcgg tgtttctcca gatgtggccc atctgaggcc
    43561 gtgttggtgc gcagctcgaa ggctacgtcg agtctgtttt ggcagtcacc ttagagtata
    43621 gatagctgac aaaaaaggct ctcgtgctaa aaaaggcccc actttcgtgg agcctttatt
    43681 gtacgctttt tactgtacga tttcagtcaa atctaattac atagattgag tgaaggtacg
    43741 agtaataacg tcctgctgct gttctttagt cagcgagttg aaacgtactg cgtagccaga
    43801 tacacggatg gtcagctgcg gatatttttc cgggttttcc atcgcgtcga gcagcatttc
    43861 acggttcatc acgttaacgt tcaggtgctg accaccttcg atggatgctt cgtggtggaa
    43921 gtaaccatcc atcagaccag ccaggttggt cttacgaact tcgtcgtctt tacccagtgc
    43981 gttcggaacg atagagaagg tgtaggagat accatcttta gcgtaagcaa acggcagttt
    44041 agcaacggaa gtcagagacg ctacagcacc tttctggtca cgaccgtgca tcgggttagc
    44101 acccggtccg aacggcgcgc cagcacgacg accgtctggg gtgttaccag ttttcttacc
    44161 atacacaacg ttagaagtga tggtcagaac agactgagtc gggatagcgt cacggtaggt
    44221 gtgcagtttc tgaattttct tcatgaaacg ttctaccagg tcaacagcca ggtcatctac
    44281 acgcggatca ttgttaccaa actgcgggta ttcgccttcg atttcgaagt cgatagcaag
    44341 accgtcttcg tcacgaatcg gtttaacttt cgcatatttg attgcagaca gggagtcagc
    44401 agcaacggac agaccagcga taccacacgc catggtgcgg ataacgtcac ggtcgtgcag
    44461 cgccatcaga gaggcttcgt agctgtactt gtcgtgcatg tagtggatga tgttcagtgc
    44521 agtgatgtac tgtttagcca gccagtccat gaagtgatcc atgcgctcca tcacttcatc
    44581 gtagttcagg agatcgcctt tgatcggttc agacttcgga ccaacctgca ttttcagttt
    44641 ttcgtcaacg ccgccgttga ttgcgtacag catggttttc gccaggtttg cacgcgcacc
    44701 gaagaactgc atttgtttac caacgatcat cgggcttacg cagcaagcga tagcatagtc
    44761 atcgttgttg aagtccggac gcatcaggtc atcgttctca tactgcagag aagaggtgtc
    44821 gatggacact ttagcggcga atttcttgaa gttcagcggc agtttttcag accacagaat
    44881 ggtcatgttc ggttccggag acggacccat ggtgtacagg gtgttcagga aacggaagct
    44941 gtttttggta accagggtac gaccgtcgag gcccatacca ccgatagatt cggttgccca
    45001 gatcgggtcg ccagagaaca gttcatcgta ttccggagta cgcaggaagc gaaccatacg
    45061 cagtttcatg accaggtggt caaccatttc ctgcgcttct tgttcggtga tcttgccagc
    45121 tttcaggtca cgttcgatgt acacatccag gaaggtggag gtacgaccga aggacattgc
    45181 agcaccgttc tgagacttaa cagcagccag gtagccgaag taagtccact ggatagcttc
    45241 ctgagcgttg gtagccggac cagagatgtc gtagccgtat ttcgcagcca tttctttcat
    45301 ctgacccaga gcgcggtgct gttcagcgat ttcttcgcgc agacggatag tctgttccag
    45361 gtttacgccg ttttccagat cagcctgcag agaagtgaac tgtgccagtt tgtctttcat
    45421 caggtagtcg ataccgtaca gcgcaacgcg acggtagtca ccgatgatac ggccacggcc
    45481 atatgcatct ggcagaccgg tcagaacacc agatttacgg caacgcagga tgtccggagt
    45541 gtaaacgtcg aacacgccct ggttgtgagt tttacggtat tcagtgaaga tttttttgat
    45601 catcggatcc agttcgcggt tgtacgcttt gcaggaacct tcgatcattt tgataccacc
    45661 gaacgggata agagcacgtt tcagcggagc ttcagtctgc agaccaacga ttttctcaag
    45721 ctgcttgttg atgtagccag cgtcgtgaga ggtgatggtg gaagcaacag cggtgtcaaa
    45781 gtcaactggc gcgtgagtgc ggttttccag tttaacgcct tccattactt tgtcccacag
    45841 ggtggtggtc gcttcagtag cgccagccag gaaggactcg tcaccctcgt acggagtgta
    45901 gtttttctga atgaagtcac ggacgtttac ttcattctgc cagtcacctt tggtaaaacc
    45961 ttcccaggct gtggctaact tttcattaag ctcggacatg taacacctac cttcttaagt
    46021 ggatttttta tttactgcgt acttcgacaa ccattaatgg tgatcgtttt cacgcaggta
    46081 aatgacccag tatgtcaacc caaccaacaa accaccgccg ataatattac cgatcgtaac
    46141 cggaatcagg ttatcagtga tgaagttcat cacggtcagg tgagaaaaat tttccggtgc
    46201 agaaccgaca gcggtccaga attccggaga tgcgaagtcg cggattacaa tacccatcgg
    46261 gatcataaac atgtttgcga tactgtgctc aaaaccgctg gcaacaaaca tcgcgaccgg
    46321 cagcaccata atgaacgctt tgtccatcag gctacggcca gaataactca tccatactgc
    46381 cagacatacc atcaggtttg ccaggatacc aaggcagacg gcctcaataa aagtatggtg
    46441 cactttgtgg tcggcggttt gtaggacgtt tagtccccat tgaccatttg cggtcatata
    46501 ctcgccggaa agccacatta aaagtacaaa cagcagtgcg ccgaccaggt tgccaaaata
    46561 gacatttagc cagtttttcg ccaactgacc ccaggtgatg cgcccactcg ccttagcaac
    46621 aacaatcaac actgtggaag taaagagatc ggctccgcag acaacacaaa gaatcagccc
    46681 cagagagaag caaatgccgc caaccagttt tgccatgccg aagggcattg tgcctgtgcc
    46741 agtggttgct gtgatataga agacgaatgc gattgagatg aaaacaccgg cggtaatcgc
    46801 cagatagaaa gtcttaagcg gatgtttcgt tgctttatag acacccgcct cttcggccac
    46861 tttggccatt gcagcaggaa gtaaaagatc aaaagggttg tcagctttca cactaactct
    46921 ctctttatta agtcggcgac gagatactaa caaagcatta tagatgagaa attgatatag
    46981 atcatatctc gcctggctta taggcccgta actcgcatgg tttt
//
LOCUS       DAPHDW010000020        45245 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.020, whole genome shotgun sequence.
ACCESSION   DAPHDW010000020 DAPHDW010000000
VERSION     DAPHDW010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 45245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.020"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(16..849)
                     /gene="fdhD"
                     /locus_tag="RH057_001080"
     CDS             complement(16..849)
                     /gene="fdhD"
                     /locus_tag="RH057_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HDV3127488.1"
                     /translation="MKKTQQKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGQLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
     gene            1043..4093
                     /gene="fdnG"
                     /locus_tag="RH057_001081"
     CDS             1043..4093
                     /gene="fdnG"
                     /locus_tag="RH057_001081"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:1628..1630,aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV3127489.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNREYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            4106..5008
                     /gene="fdoH"
                     /locus_tag="RH057_001082"
     CDS             4106..5008
                     /gene="fdoH"
                     /locus_tag="RH057_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HDV3127490.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            5005..5640
                     /gene="fdoI"
                     /locus_tag="RH057_001083"
     CDS             5005..5640
                     /gene="fdoI"
                     /locus_tag="RH057_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV3127491.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHHNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            5637..6566
                     /gene="fdhE"
                     /locus_tag="RH057_001084"
     CDS             5637..6566
                     /gene="fdhE"
                     /locus_tag="RH057_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HDV3127492.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQEVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(6896..7138)
                     /locus_tag="RH057_001085"
     CDS             complement(6896..7138)
                     /locus_tag="RH057_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127493.1"
                     /translation="MNSLVGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(7357..7575)
                     /locus_tag="RH057_001086"
     CDS             complement(7357..7575)
                     /locus_tag="RH057_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HDV3127494.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            complement(8161..8451)
                     /locus_tag="RH057_001087"
     CDS             complement(8161..8451)
                     /locus_tag="RH057_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000356400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3127495.1"
                     /translation="MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRAS
                     GLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALLRAIANDPQHVLQALNR"
     gene            complement(8452..8763)
                     /locus_tag="RH057_001088"
     CDS             complement(8452..8763)
                     /locus_tag="RH057_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV3127496.1"
                     /translation="MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGG
                     LRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIYRKGIKDDLSPQEKAMLRLLNTR
                     W"
     gene            8992..9900
                     /locus_tag="RH057_001089"
     CDS             8992..9900
                     /locus_tag="RH057_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3127497.1"
                     /translation="MALEKGIAELVEKFIAAGRPSSRQQSIAQRREGYIASAVLAGET
                     ETRVDIQTIELEGMTLRIVSPLNAPTLLPTIIYYYGGCFVSGGFATHDNQLRQLAYYG
                     QCRVIAVQYRLAPEHTFPAAHDDAQRGAEIVWQHAERLGVDKQRITLAGDSAGGHLAL
                     VTALRLKRAGEWQPAQLILIYPMLDATAHFESYIRNGHDYIITRDTLLSGFEMYLPGI
                     ERRHPEASPLWRDDFNGLPPVHIITAEYDPLCDEGEALYHRMTGQGVQCTCQRYLGVI
                     HGFFQLGGISEAARSALRDVAWRAGR"
     gene            complement(9964..10905)
                     /gene="fabY"
                     /locus_tag="RH057_001090"
                     /gene_synonym="yiiD"
     CDS             complement(9964..10905)
                     /gene="fabY"
                     /locus_tag="RH057_001090"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HDV3127498.1"
                     /translation="MYHLRVPQTEEELERYYQFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMAVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(10950..11387)
                     /gene="dtd"
                     /locus_tag="RH057_001091"
     CDS             complement(10950..11387)
                     /gene="dtd"
                     /locus_tag="RH057_001091"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HDV3127499.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(11384..12256)
                     /locus_tag="RH057_001092"
     CDS             complement(11384..12256)
                     /locus_tag="RH057_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HDV3127500.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(12250..12849)
                     /gene="yihX"
                     /locus_tag="RH057_001093"
     CDS             complement(12250..12849)
                     /gene="yihX"
                     /locus_tag="RH057_001093"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HDV3127501.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(12990..13877)
                     /locus_tag="RH057_001094"
     CDS             complement(12990..13877)
                     /locus_tag="RH057_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HDV3127502.1"
                     /translation="MKTERKNEHYLALQQAFDAPWPGPVGELVTLEKGNIHLQIYPHD
                     GARITSLKAFGSEVLRQWQPQRRAFQYGCFPMVPWAGRLGNATLNAGGQCYSLPANKP
                     PHALHGMACYSTWEIIDKTADSLTLRMPLASPWPWQGEVIQTFLLENDALVLQLEVHS
                     YADTFPASAGWHPWFAKKLTPQNTESLQVLFDADWQEEAGSDELPTGNRISPQAGPWD
                     DCFGFYDGVKVKLLWPGKLAMTMISSANSLVVFDKQPDATCINPLTQAPNAINLTPEL
                     VTPDRPLVIETRWQFTPES"
     gene            complement(13867..15216)
                     /locus_tag="RH057_001095"
     CDS             complement(13867..15216)
                     /locus_tag="RH057_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HDV3127503.1"
                     /translation="MSEKLPAPREGLSGKAMRRVVMGSFAGALMEWYDFFIFGTAAGL
                     VFAPLFYPDSDPFIGLIAAFATFGVGFLTRPLGGIVFGHFGDKIGRKITLIWTLAIVG
                     CSTFLIGFIPTYQEIGIWAPLILMALRLIQGFGLGGEYGGAALMTIESAPESRRGFLG
                     SLPQTAASVGIMLATGIFALCNHFLTSEQFLSWGWRIPFWLSAVMLIVGLFIRLHTEE
                     TLDFQKQKTTNNKEKSVPPLIELFKKHPRNILLALGARLAESVSSNIINAFGIVYISS
                     QLALSRDIPLTGMLIASAIGIFSCPLVGWLSDRIGQKSLYLSGAGFCVLFAFPFFLLL
                     DSKSTLIIWCSMILGYNLGPTMMFAVQPTLFTRMFGTKVRYTGLSFAYQFSAILGGLS
                     PLIASSLLALGGGKPWYVALFLFAVSVLSFVCVWLIEPTDEQETASYRYIREQSHEN"
     gene            complement(15315..16748)
                     /locus_tag="RH057_001096"
     CDS             complement(15315..16748)
                     /locus_tag="RH057_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3127504.1"
                     /translation="MSQITSPATYSISRPQDVIDIVNKNSAINTSIGVIFIALGGILI
                     DAYQAAMVGFGNKYIAAQFGISPGLAATVNASVLIAALIGGLLANRVINRFGQKRAFI
                     IGMGLCTIGAAAVAIAPSIWWVLVCRVIMGFGLGIDFPLATNAVAELRGSTSKKTGTS
                     VNLWQMAWYVSTTVVYLVLLPLLLSGIAEEQLWRYGIFIGAIFAVIFMILRYFFIGES
                     AMWAARVGRYQEACDILGKRYGVQARVAASSTTEAKFSEKAENKYSGGYGILFNDRYR
                     KRTILGCVVATMQAWQYNAVGVYLPLTLAGIISGGLTGALTGSAVVNALCGVTGGMIG
                     SFILQRLGTRRQSMYGFAVVTLALLSLGALATTNPWLSLGLLGSIIFFHSAGPGGLGM
                     TIATLSYPPAIRPTGVGFARAIMRTGAIAGLIFWPMLWGALKTEAFYWLAIVPFLGFL
                     TCVLINWEPLGANVDAEDAEVLAELKK"
     gene            complement(16818..17828)
                     /locus_tag="RH057_001097"
     CDS             complement(16818..17828)
                     /locus_tag="RH057_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001494590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HDV3127505.1"
                     /translation="MDKITQVLFSDIGKVTTQYVEVPHQELKPHEVRIAPVFYGICGS
                     DLHVLKGGHPFAKPPVVPGHEIAARVTEVGSDVKNVQPGDHVVVDPIMACMECRACKA
                     GRFNLCEPPQVAGFRAPGFARSQHIVPARNCHVAPASLPLKVLAFAEPAACARHCVNR
                     MPKASLESVLVIGAGTIGLSIVQALRIMGAGKITVIEPDAAKRALALKLGAAEVWAPG
                     ELAADVRFTGAIDVVAAQATLNDACTRVYAGGTVVCMGVPSGPREIPLPMMQRFERDL
                     LNSGMYIPEDFDAVIEWLADGRFDTSELVTDLFAIEDAAAAFERAQQNDSIKVMLQFA
                     PE"
     gene            complement(17861..18748)
                     /locus_tag="RH057_001098"
     CDS             complement(17861..18748)
                     /locus_tag="RH057_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV3127506.1"
                     /translation="MSSHFTPFTLKDISRPGGGFAMLAVDQREAMRLMFAAAGQPKPI
                     ADSVLTDFKVAATRILSPYASAVLADKQFCLEQIVEQGAVANSCGLIVAADLFIPGNG
                     IPVDSVEIDMSVDPHKAREMGAKAMKLLVLWREDEPAEERLAMVDKFVRRCRSAGLVS
                     IIEPVVRPPRRGWDFDRESAIVAAAAELGGTEADLYKAEMPLGGKGDEKTLLAACQQL
                     NDQMKMPWVILSSGVDADIFGRAVSIAMKGGASGFLAGRAVWASVVGAQDPQTMLRDV
                     SVPRLQRLAEIVDEGIAQR"
     gene            complement(18773..19651)
                     /gene="yihU"
                     /locus_tag="RH057_001099"
     CDS             complement(18773..19651)
                     /gene="yihU"
                     /locus_tag="RH057_001099"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001318159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HDV3127507.1"
                     /translation="MATIAFLGLGQMGSPMANNLLQKGHSLQVFDVNAQAVDALVTQG
                     ATAAQTPAEAAAGAEFIITMLPNGDIVRQVLLGEKGVCEAVSSDALLIDMSTIHPLQT
                     DALIRELQEKGISMMDAPVGRTSVNAIDGTLLILAGGTDEQIARARPILMCMGNELVE
                     AGGPGMGIRVKLINNYMSIALNALSSEAAVLCESLGLNLDVAIKVMSGTAAGKGHFTT
                     TWPCKVLKGDLSPAFMVDLALKDLRIAVDVARETGAPLNMGIAAESYYAAASQDGKGR
                     QDWSALLNQVRQQAGL"
     gene            19824..20720
                     /locus_tag="RH057_001100"
     CDS             19824..20720
                     /locus_tag="RH057_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000196715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3127508.1"
                     /translation="MTKIACVGITVQDRIYSLLTLPDGGGKYQANHYLEIGGGPAATA
                     AVAIAKLGVEVDFIGRVGDDSCGNTLLAELEGWGVNTAFCRRYPNARSSQSAILVDQH
                     GERIIVNYPSPDLGTDAEWLEAIDFSRYDLILADVRWHSGTEKAFSLARLAGVTTLLD
                     ADMTPQDISPLVALADHAVFSTPGLKRMTGLQSPEEGLFQATTQTAGKVYVTLGSEGS
                     LWIEDGHLCQQEAFSVNVVDTTGAGDVFHGALAVALAEKMPTKEAIRFASAVAAMKCT
                     QPGGRAGIPNREQTESFLSLYA"
     gene            20760..21548
                     /locus_tag="RH057_001101"
     CDS             20760..21548
                     /locus_tag="RH057_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3127509.1"
                     /translation="MSIIEVTGNPRHDQLVHLIAERGYMNIEELAQLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRVSSVMNTAFEQRELSLTAEKRAIAEAVADYLPERCTVFI
                     TIGTTVEAVARALLNRRDLRIITNSLRVAQILYKNQDIEVMVPGGTLRAHNGGIIGPG
                     AVDFIEGFRADYLITSIGAIEHDGTLLEFDVNEALVARTMIKHARNTLLVADHTKFAA
                     SAAVSIGNARNVRAFFTDAPPPNSFCQLLSEENVELVVAEQEVS"
     gene            complement(21659..22924)
                     /locus_tag="RH057_001102"
     CDS             complement(21659..22924)
                     /locus_tag="RH057_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3127510.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEAKIAENKTGAAMALGSFIGYAPAMFCFSLYGYILDLNPGIIGYKIVFGIMA
                     CFAFCGAVVSVMLVKRISQRKKEMLAAEA"
     gene            complement(23026..24006)
                     /locus_tag="RH057_001103"
     CDS             complement(23026..24006)
                     /locus_tag="RH057_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HDV3127511.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGASSVRFYPRYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEPGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYAPAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(24014..24724)
                     /locus_tag="RH057_001104"
     CDS             complement(24014..24724)
                     /locus_tag="RH057_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HDV3127512.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(24941..26764)
                     /gene="typA"
                     /locus_tag="RH057_001105"
     CDS             complement(24941..26764)
                     /gene="typA"
                     /locus_tag="RH057_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HDV3127513.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            27137..28546
                     /gene="glnA"
                     /locus_tag="RH057_001106"
     CDS             27137..28546
                     /gene="glnA"
                     /locus_tag="RH057_001106"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HDV3127514.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            28865..29914
                     /gene="glnL"
                     /locus_tag="RH057_001107"
     CDS             28865..29914
                     /gene="glnL"
                     /locus_tag="RH057_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HDV3127515.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVLDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            29926..31335
                     /gene="glnG"
                     /locus_tag="RH057_001108"
     CDS             29926..31335
                     /gene="glnG"
                     /locus_tag="RH057_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HDV3127516.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNVQLNGPTTDIIGEAPAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRAKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            31447..31557
                     /locus_tag="RH057_001109"
     CDS             31447..31557
                     /locus_tag="RH057_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HDV3127517.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(31747..33120)
                     /gene="hemN"
                     /locus_tag="RH057_001110"
     CDS             complement(31747..33120)
                     /gene="hemN"
                     /locus_tag="RH057_001110"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HDV3127518.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFQFNADAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVDEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPIEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(33309..33818)
                     /gene="yihI"
                     /locus_tag="RH057_001111"
     CDS             complement(33309..33818)
                     /gene="yihI"
                     /locus_tag="RH057_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HDV3127519.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDERLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            34400..35032
                     /locus_tag="RH057_001112"
     CDS             34400..35032
                     /locus_tag="RH057_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HDV3127520.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKAQLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(35414..38200)
                     /gene="polA"
                     /locus_tag="RH057_001113"
     CDS             complement(35414..38200)
                     /gene="polA"
                     /locus_tag="RH057_001113"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HDV3127521.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEAGKWLQAKGAKPAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISRERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKEQGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            38564..39496
                     /locus_tag="RH057_001114"
     CDS             38564..39496
                     /locus_tag="RH057_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HDV3127522.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWSLDMPFMKRYSRAYLLRH
                     PERRGKDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQDK"
     gene            complement(39537..40967)
                     /locus_tag="RH057_001115"
     CDS             complement(39537..40967)
                     /locus_tag="RH057_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HDV3127523.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGVEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTMLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVKWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKRADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(41122..41748)
                     /gene="dsbA"
                     /locus_tag="RH057_001116"
     CDS             complement(41122..41748)
                     /gene="dsbA"
                     /locus_tag="RH057_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HDV3127524.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEKK"
     gene            complement(41765..42751)
                     /gene="srkA"
                     /locus_tag="RH057_001117"
     CDS             complement(41765..42751)
                     /gene="srkA"
                     /locus_tag="RH057_001117"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HDV3127525.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFEQGIRVDSGLTPLNSYENRVYQF
                     QDEDRRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLMPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAEQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRRWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(42828..43097)
                     /locus_tag="RH057_001118"
     CDS             complement(42828..43097)
                     /locus_tag="RH057_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HDV3127526.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDLNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            43167..43751
                     /gene="mobA"
                     /locus_tag="RH057_001119"
     CDS             43167..43751
                     /gene="mobA"
                     /locus_tag="RH057_001119"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HDV3127527.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLELNGKPLWQHVADALM
                     TQLSHVVVNANRHQEIYQASGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPEDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDNKDAFINVNTPEELARWQEKR"
     gene            43733..44260
                     /gene="mobB"
                     /locus_tag="RH057_001120"
     CDS             43733..44260
                     /gene="mobB"
                     /locus_tag="RH057_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418293.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HDV3127528.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKQLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAAQTIVASQQRWALMTETPDEEELDLQFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
     gene            complement(44364..44479)
                     /gene="rrf"
                     /locus_tag="RH057_001121"
     rRNA            complement(44364..44479)
                     /gene="rrf"
                     /locus_tag="RH057_001121"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(44574..>45245)
                     /locus_tag="RH057_001122"
     rRNA            complement(44574..>45245)
                     /locus_tag="RH057_001122"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT        11346 a        11695 c        10952 g        11252 t
ORIGIN      
        1 caggacttat tttaattaat tgctcaaacg ctgcggatgg gtataaaccg ttgcccgacc
       61 cggcttacaa aaacccacca gcgtcagatt acaacgctca gccacttcta cagcaagcgt
      121 ggtcgcggca gacaccgcaa acaaaatctc tacgccgcac atcgccgact tttgcaccat
      181 ttcataactg gcacgactgg aaaccagtac cgcaccttgc tgccagcttt ccccttcttg
      241 tgaccggcgg cctaacagtt tgtccagcgc cacatggcga cccacgtctt catgcccgcc
      301 gaccagttcg ccagatggca acatccaggc agcggcgtga gtacaaccag tcagttgccc
      361 cactggctga aaatcgttga gatgacgtaa tgcatcatcc agtttgttga gatcaaacgt
      421 ctgggtgaac ggtagcggct gcaccggttt tccgatgtca ttaagttgct ccacgccgca
      481 tacgccacag cccgtacgtc ccgccagcgc ccggcggcgc tccttcaacc ccataaagcg
      541 gcggctggaa agctcaattt gtacttcaag accattacag gaaggaacga catccatgcc
      601 gaagatatcg cgcggacttt ctattatccc ctcggaaagc gaaaaaccga gcgcaaagta
      661 ctcaaggtct ttgggcgacg ccatcatcac cacatgcgaa atgccgttgt agaccagcgc
      721 aacgggaact tcttccgcga cctcatctaa ccgtgggtgt tgtaaatcat cacgccgcca
      781 taactcaatt tgacgaacac ctgtgatatt tgtcacattt tcgatttctt tttgctgtgt
      841 ttttttcact ttctcttaac cagataagaa cacacacacc aacattatgg tattctgtta
      901 caaacccttc ctggatggag ggaaattgag ccaattctgg acctttgcgg ccccttccgc
      961 aaagaaaaat aactcccact ccctgcacac gcagcaagcg aatgtaaatg ggacgtgaca
     1021 atgtcgaaac aaggagcaat ccatgcaggt cagcagaagg cagttcttta agatctgcgc
     1081 tggcggtatg gcaggcacca cggcagcggc actgggtttt gcacccagcg tagcgctcgc
     1141 ggaaacccgg cagtataaac tgctgcgcac ccgcgaaacc cgtaatacct gcacctattg
     1201 ttccgtaggc tgtgggctgt tgatgtacag cctcggtgac ggagcaaaaa acgccaaagc
     1261 atctatcttc catatcgaag gcgatccgga tcacccggtc aaccgcggtg cactttgtcc
     1321 gaaaggcgct ggcctggtgg atttcatcca ctctgaaagc cgtctgaagt ttccggaata
     1381 ccgtgcgcca ggttctgata aatggcagca aatcagttgg gaagaggcgt ttgatcgcat
     1441 cgccaaactg atgaaagaag accgcgatgc taactacatt gcgcaaaacg ccgaaggcgt
     1501 gactgttaac cgctggctct ccaccgggat gctgtgtgct tccgcgtcga gtaacgaaac
     1561 cggctattta acgcaaaaat tctcccgcgc gctgggtatg ctcgcggtcg acaaccaggc
     1621 gcgtgtctga cacggaccaa cggtagcaag tcttgctcca acatttggtc gcggtgcgat
     1681 gaccaaccac tgggtcgaca tcaagaacgc caacctcgtc gtggtgatgg gcggtaacgc
     1741 cgctgaagct cacccggtcg ggttccgctg ggcgatggaa gccaaaattc acaacggcgc
     1801 gaagctgatt gtgatcgatc ctcgctttac gcgtacggcg gcggtagctg actactatgc
     1861 ccctattcgt tccggtactg acattgcttt cctgtcaggc gtattgctgt acctgctgaa
     1921 caatgaaaaa ttcaaccgcg aatacaccga agcctatacc aacgccagcc tgatagtgcg
     1981 tgaggattac ggctttgaag atggcctgtt caccggctac gacgcggaaa aacgcaagta
     2041 cgataaatcc tcctggactt atgaactgga cgaaaacggc ttcgccaaac gcgataccac
     2101 gctgcaacat ccgcgctgcg tgtggaactt gctgaaacag cacgtttccc gctacacgcc
     2161 agatgtggtt gaaaacatct gtggtacgcc aaaagacgcg ttcctgaaag tctgcgaata
     2221 catcgcagaa accagtgctc acgataaaac tgcctcgttc ctgtatgccc tcggctggac
     2281 gcaacactcc gttggtgcgc aaaacattcg tacgatggcg atgatccagc tgctgctcgg
     2341 caatatgggg atggccggcg gcggcgttaa cgccctgcgc ggtcactcca atattcaggg
     2401 gctgacggac ctggggctac tgtcgcagag tctgccaggt tacatgacgc tgccaagcga
     2461 gaagcagacc gatctgcaaa cctaccttac cgccaacacg ccaaaaccgc tgctggaagg
     2521 ccaggtaaac tactggggca actacccgaa attcttcgtc tctatgatga aggccttctt
     2581 tggtgataaa gcgacggcgg aaaatagctg gggctttgac tggttgccga agtgggataa
     2641 aggctacgac gtcctgcagt acttcgagat gatgaaagag ggcaaggtca atggctatat
     2701 ctgccagggc tttaaccctg ttgcctcatt cccgaacaaa aacaaagtga tcggttgtct
     2761 gtcgaaactg aagttcctcg tcaccatcga cccactgaac actgaaacct ctaacttctg
     2821 gcagaaccac ggtgagctga acgaagttga ctcgtcgaag atccagaccg aagtgttccg
     2881 tctgccatcg acctgcttcg cagaagagaa cggttcaatc gttaactcag gtcgctggtt
     2941 gcagtggcac tggaaaggtg cggacgcccc ggggattgcg ctgactgatg gcgagatcct
     3001 ctccggtatc ttcctgcgct tgcgcaagat gtatgccgaa cagggtggcg cgaacccgga
     3061 ccaggtgctg aacatgacct ggaactacgc cattccgcat gagccgaagt cggaagaagt
     3121 agcgatggag agcaacggta aggcgctggc cgatattacc gatccggcaa ccggtgcggt
     3181 tatcgtcaag aaaggccaac aacttagctc gttcgcccaa ttgcgcgatg acggtacaac
     3241 ctcctgtggc tgctggattt tcgccggtag ctggacgccg gaaggcaacc agatggcgcg
     3301 tcgtgataac gccgatccgt ctggcctcgg taacacgctg ggctgggcat gggcatggcc
     3361 gcttaaccgc cgcattctgt ataaccgcgc ctccgcagat ccgcagggta acccgtggga
     3421 tccgaagcgt cagttgctga aatgggacgg cactaagtgg accggctggg atattccgga
     3481 ctacagcgca gcgcctccgg gtagcggcgt cgggccgttt atcatgcagc aggaaggcat
     3541 ggggcgtctg tttgccctcg ataagatggc ggaaggtccg ttcccggaac actacgagcc
     3601 gtttgaaacg ccgctgggaa ctaacccgct gcatccaaac gttatctcga acccggctgc
     3661 acgtatcttt aaagacgacg ccgaagcatt gggtaaagcc gataagttcc cgtatgtcgg
     3721 aaccacctat cgtctgaccg agcacttcca ctactggacc aaacacgcgc tgttgaacgc
     3781 gattttgcaa ccagagcagt ttgtggaaat cggggagtcg ctggcgaata aacttggcat
     3841 tgcccagggc gataccgtga aagtctcctc caaccgtggc tatatcaaag ccaaagcggt
     3901 ggtgaccaaa cgtattcgta cgctgaaagc gaacggcaaa gatatcgata ccatcggtat
     3961 tccgattcac tggggttatg aaggcgtagc taaaaaaggc tttattgcca atacgttaac
     4021 gccattcgtt ggtgatgcga acacgcagac gccggagttt aagtccttcc ttgtgaatgt
     4081 ggaaaaggtg taacggagac gacatatggc ttatcaatct caagatatca ttcgtcgttc
     4141 cgcgactaac ggtctgaccc ccgcgcctca ggcgcgggac ttccaggaag aagtggcgaa
     4201 actcatcgac gtcaccacct gtatcggctg taaagcctgt caggtggcgt gttcagagtg
     4261 gaacgacatt cgcgataccg tcggcaataa cattggggtg tacgacaacc ccaatgattt
     4321 aagcgccaaa tcgtggacgg taatgcgctt ctcggaagtg gagcagaacg acaaactgga
     4381 atggctgatc cgcaaagacg gctgtatgca ctgttccgat ccaggctgcc tgaaagcgtg
     4441 cccggcggaa ggtgcaatta ttcagtatgc caacggtatc gtcgacttcc agtccgagca
     4501 gtgcattggc tgcggttatt gcattgcggg ctgtccgttc gacattccgc gcctcaaccc
     4561 ggaagacaac cgcgtctaca aatgtacgct gtgcgttgac cgcgtggtgg ttgggcaaga
     4621 accagcctgc gtgaagacct gcccaacggg cgcgattcac ttcggtacga aagagtcgat
     4681 gaaaacgctg gcgagcgagc gcgttgctga gctgaaaacc cgcggttacg acaatgcggg
     4741 tctgtacgat ccggcaggcg tcggtggtac acacgtcatg tacgtgctgc accatgctga
     4801 caagccaaat ctgtatcatg gcttgccgga gaacccggaa atcagcgaaa ccgtgaaatt
     4861 ctggaaaggc atctggaaac cgctcgcggc tgttggcttt gcggctacct ttgcggccag
     4921 tatcttccac tacgtgggtg tcggtccgaa ccgtgcggat gaggaagaga ataatctgca
     4981 cgaagagaaa gacgaggagc gcaaatgaaa cgacgtgaca ccatcgtgcg ctacaccgcg
     5041 ccggaacgta tcaaccactg gatcaccgcc ttctgcttca tcctggcggc ggtgagcggg
     5101 ctgggctttt tgttcccgtc cttcaactgg ttgatgcaaa tcatgggcac accgcagctg
     5161 gcgcgaattc tgcacccgtt tgtcggcgtg gttatgtttg cctcgttcat catcatgttt
     5221 ttccgttact ggcatcacaa cctaatcaat cgggatgata tcttttgggc gaagaatatt
     5281 cgtaagatcg tcgtcaacga ggaagtaggt gacaccgggc gttataactt cggtcagaaa
     5341 tgcgttttct gggcggcgat tattttcctg gttctgctgc tggtgagcgg cgtgattatc
     5401 tggcgtcctt attttgcgcc tgctttctca atcccggtga tccgattcgc gttaatgctg
     5461 cattcatttg ccgcagtggc gttaattgtg gttatcatgg tgcatatcta cgccgccctt
     5521 tgggtgaaag gcacgattac cgccatggtg gaaggatggg ttaccagcgc ctgggcgaag
     5581 aaacatcacc cgcgctggta ccgtgaggtc cgcaagacaa cggaaaagaa agctgaatga
     5641 gtattcgcat aatcccgcaa gatgagctgg gttcgagcga gaaacgtacg gcggatatga
     5701 ttccgccgtt attgttccct cggctcaaga atttatacaa ccgccgcgcc gagcgtctgc
     5761 gcgagctggc agaaaataat ccgctgggtg attacctgcg ctttgctgcg cttatcgccc
     5821 acgcccagga agtggtgctg tacgaccatc cgctggagat ggatctgacc gcgcgcatta
     5881 aagaagccag cgcacagggc aagcccccgc tggatattca cgttctgccg cgtgataagc
     5941 actggcaaaa gctgctgatg gcgctgattg ctgagctgaa acctgaaatg agcggcccgg
     6001 cgctggcagt gattgagaat ctggagaagg catcgactca ggagctggaa gatatggcca
     6061 gcgcactgtt tgcctctgat ttctcatccg tcagcagcga taaagcgccg tttatctggg
     6121 ctgcactgtc gctctactgg gcgcagatgg ccaatctgat ccccggcaaa gcccgcgctg
     6181 aatacggcga gcaacgtcaa tattgcccgg tttgtggttc tatgccggtg tccagcatgg
     6241 tgcaaattgg caccactcag ggtctgcgtt acctgcactg caacctgtgt gaaaccgaat
     6301 ggcacgtagt gcgcgtaaaa tgcagcaact gcgagcagag cggcaaactg cattactggt
     6361 cgctggatga cgaacaggcc gcgattaaag ccgaaagctg cgatgactgt ggcacttacc
     6421 tgaagattct ttatcaggaa aaagaaccga aagttgaagc cgtggcagat gacctcgctt
     6481 ctctggtact ggacgcgcga atggagcaag aaggctatgc ccgcagttcc atcaacccgt
     6541 tcctgtttcc gggtgaaggg gagtaatctc tgacgttttc cgggtggcgc tggtcgcccg
     6601 gattacagct catagggtgt gacgacacca tctctcgtat tccgcgttac ccattcaaga
     6661 tcatgccgca attgtctgtt tgcctcgttc ttccctccaa cacatcgata aggttatggg
     6721 tatgtcactg atgacaagcg tatgattgcc tgatgcgctg cgcttatcag ccctaccaat
     6781 aaacttcaat atattgaatt tgcatgactt tgtaggccgg ataaggcgtt cacaccgcat
     6841 ccggcatgaa caacgcgcac gttgtcagca atctgattga catcattttt tcccattacc
     6901 attcctcgcg cagcttacgc tgatattcga caccatcttc ctcacaccct tgccagatgc
     6961 caggaacact ggttcttgct gtttgcgatt gatttatcag gtattgatct accgcttccc
     7021 ttaataggtc cgcacgagga agattacgct gcacctcgag atcatcaagt tgcttaatca
     7081 cctcattcga taaatcgagt aaaattctgc ccatatctat acctaccaga gagttcataa
     7141 atatctcgcc aatatcattt ttgaatctat ggagagaaaa gtacccttgt cgaatcttta
     7201 aagaaagctc atttacgaat cattttttat ttttgcgagg cgttttccag atatgatgta
     7261 acagttgcag ttattataag ttcaagtaaa acagccttgt attaatacca gaataagggc
     7321 atctgttttt ttatattcaa gaatgaaaaa tttttttcat tccttatgct ccttacactg
     7381 atcatcaaca ccaactacct cccccttttg cctgcctccc tttgctttac gcaccggaaa
     7441 ttgctgccgc tccagatatt gctcaaccgc ttcgcgtaaa attgcagatc gtgaaacctt
     7501 acgccagccc cgaagtttgt tcagccgttt aattgcctct tctgataaat gaagaaaaac
     7561 cgtattcatc gccattacca ccaccttacc tatattactc attaatgtat gtgcgaaaat
     7621 tacgccggaa aatcatttaa aaaaagcgga tttttgcatg ttgttttgtt tttgcgaggc
     7681 gttttccaga aataataaga gtgttgcagt tgtttcattt attactcaaa aatggtttag
     7741 ttatcgccca ggatgaatta tgggtgctat gaatatatta tattcagaga tatttatttg
     7801 cataatctga atggtgtacc cgaaaaatgt cagcatccta acagcacccg gatttaaggt
     7861 gaaattaatc atccatcaca gaccggtcag tactgccacc aaccggtctt tacttgttca
     7921 tcgttataat tttgttggta acagttcggg aaacaaggaa ttccttccgg aaccccccgg
     7981 tatcatccga tagcattgac gtcaatcatc agcgcgacga tgagtttttt taatcgcatc
     8041 atagggtggc gacgttcagc acgattacgg gtcatagcaa aactcctggg caggcgacca
     8101 ctatgccgct ttcacaggag ttctgaatgc tagtgctttc tgtatggcat aaccatgcct
     8161 ctaacggtta agtgcttgca gcacatgttg cggatcattg gcaatcgctc gcagcaaagc
     8221 ctttgcaggc ccggtcggtg aacgtcgccc ctgctcccag ttacgcaacg tgtctacgct
     8281 tacagaaata agatcggcaa atttagattg tgacaaccct gaagcgcggc gtatttcttt
     8341 gattttcata gcatcaactt caaaagtccg cgaaggctgt cgcgttcctt caataatttc
     8401 attcatctgg gccatgctgt gagtcaaccg ctcaaaaagt gccttatcca tttaccacct
     8461 cgtatttaac aaccggagca ttgctttttc ctggggagac agatcatctt taataccctt
     8521 acgataaata agcagtaatc tgatctcata atgtttaacc tgatgaaagt aaataaccct
     8581 gacaccagca cgtttcccct ttcctcctga aacccatcga acctttctta acccaccagt
     8641 ttcaggtatt acctcaccat agtcaggatt caaggccagg aaaaactgaa aacgactgaa
     8701 ttcatcgtcg gtcagcagtt tttgaacatc ttcagtaaaa atttccgttt cgatgaagag
     8761 cataattata cgccattggc ttagagtaac aatccttttt gtacgtcatt gacgtaccct
     8821 tgaaaccact agcttactga tagtgctcca gcaaaaccac gtctctgccg ggtttgtctt
     8881 tagatttgcg aggcgctttc cagatttttc acgcccgtga atttccctgt atccatccag
     8941 caaaaacggg tgtataagac tgtacattca tacagcaaaa taagttctac tatggcactg
     9001 gaaaaaggaa ttgcagagtt agtcgaaaag tttatcgctg cgggtcgccc ctcatcgcgt
     9061 caacaaagta ttgcgcagcg cagggagggc tatatcgcca gcgctgtcct tgcgggtgag
     9121 acagaaacgc gggtagatat tcagactatc gaacttgaag ggatgacctt acgaatagtg
     9181 tcgccgctga atgccccgac attactcccg acgataattt attactacgg tggctgtttt
     9241 gttagcggcg ggttcgcaac tcacgacaac cagctgcgcc agttagccta ttacgggcaa
     9301 tgtcgggtta ttgccgtcca gtatcggctg gcaccggaac atactttccc cgccgcccat
     9361 gatgatgcgc aacgaggtgc agaaatagtc tggcaacacg ccgaacgact cggcgttgat
     9421 aaacagcgga taactcttgc cggtgacagt gcgggcggac atctggcgct ggtaactgct
     9481 ttgcgattaa agcgtgctgg cgagtggcaa ccggcacaac tcatcttaat ttatcctatg
     9541 cttgatgcga ctgcgcattt cgaaagctac atacgcaatg gtcacgatta catcatcaca
     9601 cgcgacactt tactgagcgg ctttgagatg tatctgccag gcattgaacg caggcatcct
     9661 gaagccagcc ctctttggcg cgatgatttc aatggcttgc cacctgtaca tatcattacc
     9721 gctgaatacg acccactttg cgatgaaggt gaagcgttgt atcaccgcat gacagggcaa
     9781 ggagtgcaat gcacctgcca acgctacctt ggcgttatcc acggattttt tcagttaggt
     9841 ggtatcagcg aggccgcgcg atcggcacta cgggatgttg cctggagagc gggaaggtaa
     9901 cttcggacct gggccctgaa aaggttttca gattgaaaac cttttcagtc aagtaaaacg
     9961 cccctactct tcttcgttcc cgccctcttc atacgggcca aatggcttcg cgggcagaac
    10021 gatatacgtg ccttcaaaca ctgcacccgg cgtctcgtcg ccaaagattt caacttgcat
    10081 ctgcacccgt gcttttcgtc cgcgcgccag acggtcgaga tcgccgctta aggcaccaag
    10141 gtcggctacc gcatgaggtt taccgctaat cggcttgctg tagcggatat gcgcatccgc
    10201 cagaataatc gttccgccga gatggcgttc gcgcagcatc agccagataa gcccccaacc
    10261 ggtgagcgtc gccagcgaga ataaactccc ggcaaacagc gtatggtgcg gattctgatt
    10321 gccggtttcc ggcatggtgg tgataaattt ttgcccggta tattgttgaa tacgcacgcc
    10381 cattttttca ctaagcggga tatgttcgta ccacgcctgt tgcagctgcg cgcaccagtc
    10441 gccgcgatgc aaaatatcat ccagagtggc gacgggctta atcatcaaaa aatggcgaat
    10501 cggcgtggtg gttggcgtgg tgatttctcc ctgattaaca aaccccagct tggcgaaaaa
    10561 ctccaccgcg tcttcacggg cgctacaggt cacgcgctta acgccttcct gacgcgccac
    10621 cgactccagg gtcatcgcca tcagcgttcc taaccctttg tcctgcacgt ccgggtgaac
    10681 ggccataaag cgaatggacg cttcattgtc ggcattaata tacagtcggc ctaccgccac
    10741 cagattaccc tgctcgtcga cgaccatctg gtgatgcgcc atcgcatccc acgcgtcgcg
    10801 ttccgaacct tttggttgat gcaggggctt acgcaacatt tcccagcgaa actgatagta
    10861 acgctctaat tcttcttctg tttgtggaac ccgaaggtga tacatagcga tactctctct
    10921 tgtttcccgt gacaaccctg gaagctggct catacctgca accagaatgt cacggggcca
    10981 tcattgacca gcgatacctg catatcggca gcgaagcgtc ctgtttgcgt gttcatctct
    11041 tgctggcggc agcgttcgac gaaatagtca tataacgcct ctgcgcgatc cggtgatgcg
    11101 cctttggaga aacttgggcg catccctcgt tcggtatctg cggcgagggt aaactgggaa
    11161 accaccagta cactgccgcc cgcctgttgc acgttgagat tcatcttgcc ttcggcatcg
    11221 ctaaagatgc ggtagccgag cacacgctcg cacagacggt ttgctttctg ttcgtcgtca
    11281 tccttttcga cacccaataa caccaaaagt cccgcgccaa tttcgcccgt cacttctccc
    11341 tccacggtga cgctggcacg ggttacgcgt tgaattaatg caatcatggt tcgtcgtctt
    11401 cttcttgttc agctgcttgt tttagtttgc ggtattcccc gagagtgaca gtaatttccg
    11461 cgccaagcaa gacgatacac catgtccagt agacccaaac aaagagaatg gggatcaccg
    11521 ccagcacacc gtaaatgagc tgatatgacg ggaacatggt gatataaagc gcgaaacctt
    11581 tctttcctgc ttcgaacagg agcgcggcga caaacgcgcc gacaatcgcg tcgcggttag
    11641 gtacgcggat ggtaggaaca atgctgtaca gcaaccagaa ggagatccac gacaacagca
    11701 gcggaaaaat acgcagcacg ttgtcgatga cagtattgag atcgctcgcc cagcgcaggg
    11761 agagcaaata ggaactgatc gccagactgg cccctgccag cagcggcccc agcgttaaaa
    11821 tcatccagta cacggcgaac gagtaaattt ttggtcgcgc tcgtttactg cgccagatgg
    11881 tattcaacgc gctatcgatg gagtacatca gcaataacgc ggtgacgatc agcccgcacg
    11941 ccccaacggc ggtcatcttg ttggaattgg caacaaattg ttcgatatac cgctgaataa
    12001 catcgccagt agcaggcaga aagttggcaa aaataaagtg acgtaactga atgctgacgt
    12061 cggaaaacat gggaaaagcg gcgaaaagcg caaaaacaac ggcaaccagc ggcactaatg
    12121 agagcaacga cacataggca aggttacctg ccagggttgt catgttgtcc tcatcaatgc
    12181 gttgccagag tagttttagc caggcccata gtggacgggt acggtgcctg gctttgtcct
    12241 gaatggtttt tagcataaca ccttcgcgaa atagtccggg atggtggttt tatctttcac
    12301 cagaatactg gtaatgccca gctgattggc tccttctata ttatcggcgt tatcgtcgaa
    12361 aaagaccgta tcgctgggtg aaaaaccttc cgcctgcaaa acatgctggt aaattcgtgc
    12421 ttcaggtttg cgcatcccca gatcttgcga cagatagata tggtcagcag catcacgaat
    12481 ttccgggtat tcttccggcc agaaggtggt atgcaggcgg ttggtattgg aaagcaccac
    12541 cacgcgatgc ccctgctcac gcagtttatg catgatggcg atcacttccg ggcgcagcgc
    12601 aacaaacacc gcctgccagc cgtgagagaa ctgctcgtag cttagcggta gagccatctc
    12661 atgacacagc gcctctgcga acgcttcgtc gctaatttcc ccacgctcat gctgatgaaa
    12721 cgcctccccc atatgaaaac tcttcttaag cgatgccagc ggaatacgcg ttaaatcgct
    12781 ccaggctccc agcacacggt taaagtcgat atcgacaatc acattaccta aatcaaagat
    12841 atagagcatt tttgcctcct ttcgcgccac aagaaattaa ctgtagcggg aaaggatggg
    12901 tttggctatg tggcaataag ggtaatcttg tgatgctgat tgcgaaaaag cagagtaatt
    12961 tcactggctt tttcgcaata gatgaatggt taagattcag gtgtaaattg ccaacgagtt
    13021 tcgatgacca gcggcctatc tggtgtcacc aattccggcg ttagatttat tgcgttaggt
    13081 gcctgagtca gcgggttaat acaggtggca tcgggctgtt tatcaaatac caccagactg
    13141 tttgcagaag agatcatggt catcgcgagt ttaccaggcc acagtaactt aaccttcacc
    13201 ccgtcataaa aaccaaaaca gtcatcccag ggaccggctt gaggagagat acgatttcca
    13261 gttggcagtt catcactacc cgcttcttct tgccagtccg catcaaaaag cacctgcaac
    13321 gattctgtat tttgcggagt cagctttttc gcaaaccacg gatgccagcc tgcggaggcc
    13381 gggaatgtat cggcatatga atggacttcc agttgcaaaa ccagcgcatc gttctccagc
    13441 aaaaaagtct gaatcacttc cccttgccag ggccacggtg aagccagggg catacgtaaa
    13501 gtcagggaat ctgctgtttt gtcgatgatt tcccacgttg agtaacaagc cataccatgc
    13561 aacgcatgtg gtggtttatt ggcgggtaaa gaataacatt gcccaccagc attcagcgtc
    13621 gcattcccca gtcgccctgc ccacggcacc attgggaaac agccatactg aaaagctctg
    13681 cgctgcggct gccattggcg taatacttca gaaccaaatg cctttaacga ggttatcctg
    13741 gcaccatcat gcggataaat ctgcaggtga atattgcctt tttccagcgt cactaactct
    13801 cctacaggcc caggccatgg cgcgtcaaac gcctgttgta gcgccaggta atgctcgttt
    13861 tttcgttcag ttttcatgag attgttccct gatgtagcgg taagaagcgg tttcttgttc
    13921 atcggtcggc tcaatcagcc agacgcaaac aaaggaaaga actgagacag caaataaaaa
    13981 cagagcgaca taccacggtt ttcctccccc tagcgccagc aaacttgagg cgataagggg
    14041 gcttagtccg ccaagaatcg cggaaaattg ataagcaaaa gaaaggccgg tatatcgtac
    14101 tttggtaccg aacatgcggg tgaaaagtgt tggctgcacg gcaaacatca tcgtcggacc
    14161 taagttgtag ccgaggatca tactgcacca gatgatgagc gtacttttgc tatccagtaa
    14221 taagaagaaa ggaaacgcga acaggacaca aaatcctgct cccgacaaat acagactttt
    14281 ttgacctata cgatctgata accatccaac caatggacaa ctgaaaatac cgatcgccga
    14341 ggcaatcaac atacccgtca ggggaatatc tcgcgacaat gctaattggc tggaaatata
    14401 gacaatacca aaggcgttaa taatattaga ggagacactt tccgccagcc tcgcacccag
    14461 tgccaataaa atatttcgtg gatgtttttt gaataattca atcaacggag gaacggactt
    14521 ttctttatta ttcgtcgttt tttgcttttg aaaatccagc gtttcttcgg tatgcaggcg
    14581 gataaacagc ccaacgatca acatcaccgc agaaagccaa aacggaatgc gccatcccca
    14641 ggagagaaac tgctccgaag taagaaaatg gttacagaga gcaaaaatac cggttgccag
    14701 cataataccc acagacgcag ccgtttgcgg gagtgaacct aaaaatccgc gtcgggattc
    14761 gggcgctgat tctatggtca tcaatgccgc tccgccatac tcgccaccaa gaccaaagcc
    14821 ctgaattaaa cgcagcgcca ttaatatgag cggtgcccag atcccaattt cctgatacgt
    14881 cggaataaaa ccaatcaaaa atgtggagca accgacaata gccagtgtcc agataagtgt
    14941 tatttttcgc ccaattttat cgccaaaatg gccaaagacg ataccaccta aaggacgggt
    15001 caaaaaacca acgccaaagg tagcaaaggc ggctataaga cctataaaag gatcgctgtc
    15061 tggataaaac agcggtgcaa ataccagtcc tgctgccgtg ccaaaaataa agaagtcata
    15121 ccattccatt aacgcgcctg cgaaactgcc cataacgaca cgtctcatgg ctttaccgga
    15181 aagcccttca cgcggtgcgg gtaacttttc agacataatt ttcctctcct ggtaggcgag
    15241 aaatctggga tacgaaccac taaacgtggg cagtttgaat taatgcgcat cggtttaagc
    15301 cgatgcgcat taatttattt cttcaattca gccagaacct ctgcatcctc agcatcaaca
    15361 tttgcaccca gtggttccca attaatcaat acgcaggtca ggaaccccaa gaatggcacg
    15421 attgccaacc agtaaaacgc ttcagttttc aacgcccccc acagcatcgg ccagaaaatg
    15481 agtcctgcaa ttgcccctgt gcgcataata gcgcgggcaa atccgacccc agtagggcga
    15541 atagcgggag gataagagag tgtggcaatg gtcatgccca gcccaccagg acccgctgaa
    15601 tggaagaaaa taattgatcc caataaccct aaagacagcc atggattagt cgttgccagt
    15661 gcgcctaacg acagcaatgc taaggtcaca acagcaaatc catacatcga ctgtcggcga
    15721 gtacccagtc gttggagaat aaacgagccg atcatcccgc ctgtcacccc acaaagggca
    15781 ttcacgacgg cagaacccgt caacgcacca gtaagcccgc cacttattat tcccgccaac
    15841 gtaagaggaa gataaacacc tacggcgtta tattgccacg cctgcatggt tgccacgaca
    15901 cagccaagaa tggtgcgttt gcggtaacga tcattaaata agatgccata tccaccactg
    15961 tatttattct ctgctttttc cgagaatttc gcttctgttg tactcgatgc cgcaacgcga
    16021 gcctgaacac cataacgttt tcccagaatg tcgcacgctt cctggtaacg cccgacgcgt
    16081 gcggcccaca ttgcggattc accaataaag aagtaacgca aaatcatgaa gatgactgca
    16141 aaaatagctc cgatgaatat tccgtaacgc cacaattgtt cttcagcgat acccgacaga
    16201 agcagcggca agagcaccaa ataaacaaca gttgtcgaaa cataccaggc catttgccag
    16261 aggttgaccg acgttccggt tttcttcgac gttgaaccac gaagctctgc cacggcattg
    16321 gttgccaaag ggaagtcgat gcctaaacca aagcccatga tgacgcggca caccagcacc
    16381 caccagatac tgggcgcaat agctaccgca gcagccccga tggtgcacag ccccatgcca
    16441 ataataaatg ctcgcttttg cccaaagcgg tttattactc ggttcgctaa taaaccgcca
    16501 attaacgcgg cgattaatac tgacgcatta acggttgcgg caaggcctgg agaaatgcca
    16561 aattgagcgg caatgtattt attaccaaac cccaccatcg ccgcctggta ggcatcaatc
    16621 aatatgccgc ccaaagcaat aaaaataacg ccgatactgg tgttgattgc agagttctta
    16681 ttaacaatat caattacgtc ctgtggccga ctaattgaat aagttgcagg actggtgatt
    16741 tgactcatcg cagtgtcctt tttaattgtt gtagggtggc gctgacgtga cgcagcgcca
    16801 gacagagatt tagaaaatca ttccggcgca aattgcagca tgacctttat ggagtcattt
    16861 tgctgcgcgc gttcgaatgc cgccgctgca tcctcaatgg caaataaatc ggtaaccagt
    16921 tcactggtat caaaccgccc atccgccagc cattcgataa cagcatcgaa atcttcaggg
    16981 atgtacatgc cagagttgag caagtcacgc tcgaaacgtt gcatcatcgg taatggtatt
    17041 tcacgcggcc cacttggtac gcccatgcac acgacggtgc ctccggcata tacacgggta
    17101 catgcatcgt taagcgtggc ctgcgctgca acgacatcaa tcgcccccgt aaatcgcaca
    17161 tctgcggcca gctcacctgg tgcccaaact tctgctgcgc ccagctttaa cgccagcgcg
    17221 cgtttggcag cgtcaggttc aatcacggta atcttacctg cccccataat gcgcagtgcc
    17281 tgcacgatgg ataagcctat cgttccggca ccaatcacca gtacgctttc cagagaagct
    17341 ttcggcattc ggttaacgca gtgacgggca caagccgccg gttcggcaaa ggccaacact
    17401 tttagcggta aagaggctgg tgcgacatgg caattacgcg caggaacaat gtgttgtgag
    17461 cgagcaaagc ccggtgcgcg aaaaccagca acctgcggtg gttcacaaag attaaaacgt
    17521 cctgctttgc aggctcggca ttccatgcaa gccatgatgg gatcgaccac aacatgatcg
    17581 cccggctgta catttttaac gtcgctgcca acttccgtaa cgcgcgctgc aatttcatga
    17641 ccggggacga caggtggttt agcaaacgga tgaccgcctt tcagaacatg cagatccgaa
    17701 ccgcatatcc catagaacac aggcgcaatc cgcacttcgt gcggtttaag ttcctggtgt
    17761 ggtacttcaa catattgagt ggtgactttc ccaatatctg aaaataaaac ctgggtaatt
    17821 ttatccattg ttattctcca gaatcgatat ccatagggga ttagcgttgc gcgatacctt
    17881 catcgacaat ttctgccaac cgttgtaaac gaggaacaga gacatcgcgc agcatagttt
    17941 gtggatcttg tgcaccaaca accgacgccc aaaccgctcg tccagccagg aagcctgacg
    18001 caccaccttt catggcaata cttactgcac ggccaaaaat atcggcatca acgccagaag
    18061 aaagaattac ccacggcatc ttcatctgat cgttaagttg ctgacaggcg gcaagtaagg
    18121 ttttttcatc gcctttgccc cctaatggca tctcggcttt gtaaagatca gcctctgttc
    18181 caccaagctc tgcggcggcg gcaacaatgg cgctttcacg atcaaagtcc caaccgcgac
    18241 gaggtggacg caccacaggt tcaataatgc taaccagccc ggcactacgg cagcgacgaa
    18301 caaatttatc aaccattgcc agacgttcct ctgcgggttc atcttcacgc cagagtacaa
    18361 gtaatttcat tgccttagcc cccatttcac gggctttatg cgggtcgacg gacatatcta
    18421 tttcaacgct atcaaccggg atgccattac ctgggataaa aagatcggca gcgacaatca
    18481 ggccgcaaga attagcaaca gcgccctgct cgactatttg ctcaagacaa aattgtttat
    18541 ccgccagaac ggcagaagca taaggagaaa ggattcgggt cgcggcaact ttaaaatcgg
    18601 tgagtacact gtcggcgatg ggttttggct gccctgctgc cgcaaacatc aagcgcatgg
    18661 cctcacgctg atcgacagct aacatggcaa atccaccacc tgggcgggag atatctttca
    18721 gcgtaaatgg ggtgaagtgg gatgacatag aaactcctgt taatagataa aattaaagac
    18781 cagcctgctg gcgaacctga tttagcaggg cactccagtc ctgccgccct ttgccgtctt
    18841 gacttgctgc agcgtaataa ctttctgctg caatccccat attgagcggt gcccccgtct
    18901 cccgagcgac gtcaactgca atacgcagat ctttcaacgc cagatccacc atgaaggcgg
    18961 gtgaaagatc gcctttcagc actttgcaag gccaggtggt cgtaaaatgt cctttccctg
    19021 ctgccgtacc actcatcacc ttaattgcga catcaagatt taatcccagt gactcacata
    19081 acacagcggc ctcggaggac agcgcattaa gcgcaatgct catatagttg ttaattagct
    19141 tcacgcggat ccccattcct gggccgccag cctcgaccag ctcattaccc atgcacatga
    19201 gaatcgggcg tgcacgtgca atctgctcat cagtgccacc cgctaaaatc agcaatgtgc
    19261 catcaatcgc attcacggac gttctgccca cgggggcgtc catcatactg atgccttttt
    19321 cttgtaactc ccgtattaat gcatcggtct gtaagggatg aatagtcgac atatcaatca
    19381 ataacgcgtc agatgaaaca gcctcacaga cgcctttttc ccccaacaga acctgtcgaa
    19441 caatgtcacc attgggaagc atagtgatga taaattcagc gcctgctgcc gcttccgctg
    19501 gggtttgtgc cgcagtggcc ccctgcgtaa ctagcgcatc gacggcctgt gcattgacat
    19561 caaatacctg caatgaatgc cctttttgca atagattgtt agccattggg ctacccatct
    19621 gccccagccc gagaaatgca atggttgcca tgaatactct cctgtctgac attgacaaaa
    19681 taatgacaat tgaaaattcg ttttcgtctg tttttgagca tatttgtaat ttatgttcaa
    19741 aaacacgaca cgcatcacta atttgatcat ttgtgtcatt taaattgacc gaagacgaca
    19801 aaattaaaca ggacaagaca aatatgacca aaattgcatg cgtgggtatc acagttcagg
    19861 acaggattta ttctttgctc actctgccgg atggcggcgg aaaatatcag gcaaatcatt
    19921 atcttgagat tggtggcggc ccggcagcaa cagccgctgt tgcaatcgca aagcttggcg
    19981 tagaggttga tttcatcggt cgcgtcggcg atgacagttg tggaaatacc ttacttgcag
    20041 aacttgaggg ttggggcgtc aataccgcat tttgccgccg atatcctaac gcccgttcat
    20101 cgcaatcagc gatcctcgtt gatcagcatg gcgaacggat tatcgttaat tatccaagtc
    20161 ccgatctcgg tactgatgcc gaatggttag aagcgatcga tttctctcgt tatgatctga
    20221 ttctggccga tgttcgctgg catagcggta ctgaaaaagc attctcgcta gcgcgtcttg
    20281 cgggtgtgac aacgctttta gacgccgata tgacaccgca ggatatttct ccacttgtcg
    20341 cgcttgccga tcacgccgtg ttctcaacac cagggctgaa acgcatgact ggcctgcagt
    20401 cacctgaaga aggtttgttc caggcaacga cacaaacagc aggtaaagtg tatgttacgc
    20461 tgggttctga aggatcgttg tggattgaag atggtcattt gtgtcaacag gaggcatttt
    20521 ctgtcaatgt ggttgatacc actggagcag gcgatgtttt tcacggcgca ctcgcggtag
    20581 cgttggcaga aaaaatgcca acaaaagagg ctattcgttt tgccagtgcg gttgccgcga
    20641 tgaaatgtac ccagcctggc ggtcgggccg gcattcctaa tcgtgagcaa accgaatcat
    20701 ttttgtcact ttatgcgtaa aatggtaaac atctaccgag gaggggcaag ggagtattca
    20761 tgagtatcat cgaagtgaca ggtaatccac gccatgacca actcgttcat ctaatcgcag
    20821 aacgcggtta tatgaatatt gaagaactgg cacaactgct ggatgtttca acgcaaaccg
    20881 tgcgccggga tatccgaaaa ctcagtgagc agggcttgat cacccgtcat catggagggg
    20941 ccgggcgcgt ctccagcgtc atgaataccg cttttgagca acgggaactt tcgctcaccg
    21001 ccgagaaacg ggcgatcgct gaggcagtcg ccgattacct tcccgaacgc tgtaccgtct
    21061 ttatcaccat cggtacaacc gtagaagccg ttgccagggc attactcaac cggcgtgatt
    21121 tacgcattat cactaacagc ctacgtgtgg cacagattct ttataagaat caggatattg
    21181 aagtgatggt gccgggaggc actttacgcg ctcataacgg cgggattatc ggcccaggag
    21241 ccgtggattt tattgaaggc ttccgcgcag attatttaat caccagtata ggtgccattg
    21301 aacatgacgg caccctactg gaatttgatg taaatgaagc gttagtcgcg agaacgatga
    21361 ttaaacatgc gcggaataca ttgttagtgg ccgatcatac gaagtttgcc gcgtctgctg
    21421 ccgtttcaat tggcaatgca cggaatgtca gggctttttt tacagatgcc ccgcctccca
    21481 attctttctg ccagttgtta agtgaagaga atgttgaact ggtggttgcc gagcaggaag
    21541 tttcctgaaa ttgaaacttc gaaaaatccg ctggggattc agcggatttt attttgccgg
    21601 atgcggccta cggttgatac ggttttcgtt ggccggatta agcacatccg gccattaatt
    21661 aagcttcagc cgccagcatc tctttcttac gttggctaat acgcttaacc agcattacgg
    21721 aaaccaccgc accacagaat gcgaagcagg ccataatgcc aaacacgatt ttgtagccaa
    21781 taatccccgg atttaaatcc agaatgtagc catacagact gaagcagaac atcgccggag
    21841 cgtaaccaat aaagctaccc aacgccatcg ccgcgcctgt tttattttca gcaatttttg
    21901 cttcgccgat aggtgcaaaa aatacggctc gctgtgtaaa gactatcgcg ccaaagccca
    21961 gcgtacatgc catccctaaa tagaccggca tactttcgtg cggcagcata atcaacagta
    22021 cgagcgcagc ggtactgatg ataaaggtgt agcatagata tttactcggc gattttagga
    22081 tcttatctga aatcatgcca ccaatcggtc caccaatcat tttcagacag tattggttaa
    22141 tgatgccgta agcccccacc agcgcaacgg gcaatagata gatgtttttc aggaatggaa
    22201 tgaagaatgt caggccacag taaaccgcgt aaacgaagaa gacgttaaaa gcgataagcc
    22261 agatggtttt gtctctcagc accgaggtca tactggtgtt ttgcgatgct ccgtcttctt
    22321 ttttcacctc aacggacggt gcctcttctt tgtcattcag gacaaagaaa ataataatgc
    22381 ctacggcaat cacaataagg gaatagaacc agatgcctgc tttaaaaccc aataagccac
    22441 tgccaaacca ggtaaatact gccaacgcag aaaatgccac cacggtatcg acaatgccac
    22501 gccctgtttc gaagaagcca aacaaccgac cttgttgttc gctattgccc aatcgactta
    22561 ccgacttgag caagaccggc cagttcatca tgtcgcaagt aacgccaaat agcgcccaga
    22621 caaagaggat gccccaatac cccggcattg tcgtcaaata aacacccagt aatcctgtcg
    22681 caatgagtga gaatgacatg gtgtatctgc gcggtagttt atcggcaaaa taaatagaca
    22741 gaaaaaaacc cactgtcgtg acaaatgagt ttaccgacat agcattacca atttgaccat
    22801 tggtcaaatg gaaatattcc tgcatcggga tataaaacgc atctttcagc gacggtaatt
    22861 tataaattgt accgccacac aatgttaata gactaaataa cgcccatttc tttttcgtga
    22921 gcataacatc ccctaagcga tttttaaaat cactgtttta aaggcaataa gataccgacc
    22981 attcatatgg aatggtttta ttaactgcgt atgagttaaa acttattagt ggttcagaag
    23041 atggtttgcc tcttgtttga tttgttgtag tacgttacgc acatgattta tctgattaat
    23101 tgcatcttct ttttctttac gtaaccgcgc atccagtaaa tgattttctg gcaatggtgt
    23161 ttcactcatc tggcgataag ggatccacgg attatcatct tcgtcttcaa agcggaacgc
    23221 cggcgcatag taatcaacct cttcttccag gccatatgcc gtcacctgcg gctcatcatc
    23281 acccaggcat ataaggtgcg ttaataacgc tttgaacggc atatcaccct gacctgaaat
    23341 acaggcttta tgacccaggc cacccggttc tttaacgatc aaggcatctt tgatatggac
    23401 ctgggtaata tgcggcgcca tcgtttttaa agcgtcgatg ggatgctcat ttgcattgat
    23461 catgttcgca aaatcaaaca gtaaggaaag agattccatc tcactttctt tgaccagcga
    23521 caccagctca tggcttttta aatcttcatg ctgttcgatc gtaaaagtca ggccgctgtc
    23581 ctgatacgtt tcccgtacat aggcaatgtc gttagcgatg atcgataata cgtcgcgcaa
    23641 attaccttca taacgtggat aaaaacgtac ggacgatgcc ccagttttca acgcaatggc
    23701 gacggcttcg tcaatagatg ccttatctga ggcgctggtt tcaatatgaa tatcaaggtt
    23761 cagacggcgg gctttatctc caaaggcaga gagttcttta tcgtccatat taccaagaga
    23821 aaaacgctcg ccatccagaa catggatttt gacgccacgt agattatttt cacttgcgat
    23881 atcaagtaaa tcagcaggca acactctttc cagccgcata tttaaatgaa aggcataagc
    23941 atgtaaataa agaggaagag tgtcgacacg ttgtagaatc tttcttgcgt tatttattgt
    24001 caccataaaa cacctacaga ttaatgatga ggtctacggc ctcaaatcgc caatattcaa
    24061 tatctatctg gacaatattg ccttgctggt cggcgcgatg cttttcaatt aagatcgctg
    24121 gcatacccgc agaaccgcca agatacttgc tgatatcgcc cggcattctg acgggtttga
    24181 aaatcaattt ttttactacc gtctcttttt ggtagcactt ttcccagact gcagaaaatg
    24241 agtggttttc aagttgttca ataaaacccg gagcaacttc aggattaata tatgtttcgt
    24301 gatagaaaac tttatgtcct tccagcgccc cccagccggt gatgcgatag agttcagttg
    24361 atggtgtcac tgcaatcagt ggcgcgagcg tctcgggaat atcgctggta cggtttttct
    24421 cggtaaaccc ccaggaaggc tctcgtcctt gctcaattgc ggcccgctga aagctcgccg
    24481 acaactccgg actgtaatta aatcgcggct gggtcacgaa ccacccctta cgatccttac
    24541 ggaagatttt ggattctgcc tcgaggttca acaacgcctg gcgcagcgtc atacgtttaa
    24601 tgcccagcag ttctcccagc tctctttctg aagggagttt tccacccggc gtggtgatgc
    24661 catctttcaa ccaccgatcc agtttctctt ttgcattttc tacggtggat tgattctccg
    24721 ccattgttta atccttttgg tttaaaccag atgaagcatc ttacgcttac gccaaaaaag
    24781 aaagcaatta acggcgtatg actttgcaga taattgtgac aaagctctct attgacgtaa
    24841 tcccagactt ataagggaga tactcgcatt ggtgtgcgct cacgacaggt aaaaaaaaac
    24901 ctgccagcga tggcaggttt tttataacta aaaaacgaaa ttaatcgtct ttcggtgcgc
    24961 ggttggcgcg gcgacgatcg ttttccgtca ggtgacgttt acgaatacgg atagaggtcg
    25021 gagtcacttc taccagttcg tcatcatcga tgaactccag agcttgttcc agagtcatgc
    25081 ggataggcgg aaccagaaca acggcttcgt cagtaccgga agcacgcatg ttggtcagtt
    25141 tcttaccggt caggcagttt accgtcaggt cgttagagcg gctatgaata ccgataatct
    25201 gaccttcgta aacttctgca ccgtgaccga ggaacagctt accgcgatcc tgcagaccga
    25261 acagcgcgaa cgcgaccgct ttaccctgac cgttagagat cagtacgccg ttctgacgct
    25321 gacccacttc acccggacgt acgtcgtcgt agtggctgaa ggtggagtac agcagaccag
    25381 taccggaagt catggtcatg aactcagaac ggaagccaat cagaccacgg cttgggatca
    25441 cgtagtcgag acgtacgcgg cctttaccgt ctggattcat gtttttcagg tcgcctttac
    25501 gttcgcccag cgcctgcatt acagaaccct gatgctgttc ttcaacgtcc agagtcacgt
    25561 tttcatacgg ctcttgttta cgaccgtcga tttcacggaa gataactttc ggacgggata
    25621 ccgccagttc gaaaccttca cgacgcatgt tttcgatcag aacagacagg tgcagttcgc
    25681 cacgaccaga aacgcggaac gcatcggcgt cttcggtttc ttctacgcgc agcgcaacgt
    25741 tgtgtaccag ttctttgttc agacgatcca ggatctgacg agacgttacg aacttacctt
    25801 ctttaccgca gaacggcgag gtgttaacgc agaagaacat agaaacggtc ggctcatcaa
    25861 cggagagtgc cggcagcgct tcaacgtttt gcgtgtcgca aacggtgtca gaaatgttca
    25921 gttcgccaag gcccgtgatc gcaacgatat cgccagcttc cgccagatcg gtttcgatac
    25981 gttccagacc gaggtggccc agcactttac cgactttcgc gttgcgggtt ttgccttcgc
    26041 tatcgatgat agtgacctgc tggttcggct tcactttacc gcgcttgatg cggccaatgc
    26101 cgataacgcc aacatagctg ttgtaatcga gctgagaaat ctgcatctgg aacggaccgt
    26161 caaggtcaac gtccggcgca ggaacgtggt caacaatcgc ctggtacagc ggggtcatgt
    26221 cttccgccat atcttcgtgg tccagacccg cgataccgtt cagcgcagaa gcgtaaacga
    26281 tcgggaagtc cagctgctcg tcggtcgcgt cgaggttaac gaacagatcg aatacctgat
    26341 ccacaaccca atcaggacgc gcgccagggc ggtcaacttt gttgataaca acaatcggct
    26401 tcaggccgta agcaaacgct tttttggtta cgaagcgcgt ttgcggcatc gggccgtcaa
    26461 atgcgtcaac caccagcagc actgagtcta ccatggacat tacacgttca acttcaccac
    26521 cgaagtcggc gtgccccggg gtatcaacga tgttgatacg gtaatcattc catttgatag
    26581 cggtgttttt cgcgaggatg gtaatcccac gctctttctc caaatcgttg gagtccatca
    26641 cgcgctcttg ggtttcggca cgagagtcga acgtaccgga ttgttggagc agcttgtcta
    26701 ccagggtggt tttaccatgg tctacgtgcg cgatgatggc gatattacgc aatttttcga
    26761 tcacaacttt gcctcaggca ttagaaatag cgcgttattg tacacggatt aatcgcacta
    26821 caaaacagga tcacaaacat cctccgcaaa caagtattgc agagtccctt tgtgatcgct
    26881 ttcacggagc ataaaaaggg ttatccaaag gtcattgcac caacatggtg cttaatgttt
    26941 ccattgaagc actatattgg tgcaacattc acatcgtggt gcagcccttt tgcacgatgg
    27001 tgcgcatgat aacgcctttt aggggcaatt taaaagttgg cacagatttc gctttatctt
    27061 ttttacggcg acacggccaa aataattgca gatttcgtta ccacgacgac catgaccaat
    27121 ccaggagagt taaagtatgt ccgctgaaca cgtactgacg atgctgaacg agcacgaagt
    27181 gaagtttgtt gatttgcgct tcaccgatac taaaggtaaa gaacagcacg tcactatccc
    27241 tgctcatcag gtgaatgctg aattcttcga agaaggcaaa atgtttgacg gctcctcgat
    27301 tggcggctgg aaaggcatta acgagtccga catggtgctg atgccagacg catccaccgc
    27361 agtgattgac ccgttcttcg ccgactccac cctgattatc cgttgcgaca tccttgaacc
    27421 tggcaccctg caaggctatg accgtgaccc gcgctctatt gcgaagcgcg ccgaagatta
    27481 cctgcgttcc actggcattg ccgacaccgt actgttcggg ccagaacctg aattcttcct
    27541 gttcgatgac atccgtttcg gatcatctat ctctggttcc cacgttgcta tcgacgatat
    27601 cgaaggcgca tggaactcct ccacccaata cgaaggtggt aacaaaggtc accgtccggc
    27661 agtgaaaggc ggttacttcc cggttccgcc ggtagactct gctcaggata ttcgttctga
    27721 aatgtgtctg gtgatggaac agatgggtct ggtggttgaa gcccatcacc acgaagtagc
    27781 gactgctggt cagaacgaag tggctacccg cttcaatacc atgaccaaaa aagctgacga
    27841 aattcagatc tacaaatatg ttgtgcacaa cgtagcgcac cgcttcggta aaaccgcgac
    27901 ctttatgcca aaaccgatgt tcggtgataa cggctccggt atgcactgcc acatgtctct
    27961 gtcgaaaaac ggcgttaacc tgttcgcagg cgacaaatac gcaggtctgt ctgagcaggc
    28021 gctgtactac attggcggcg taatcaaaca cgctaaagcg attaacgccc tggcaaaccc
    28081 gaccaccaac tcttataagc gtctggtccc gggctatgaa gcaccggtaa tgctggctta
    28141 ctctgcgcgt aaccgttctg cgtctatccg tattccggtg gtttcttctc cgaaagcacg
    28201 tcgtatcgaa gtacgtttcc cggacccggc ggctaacccg tacctgtgct ttgctgccct
    28261 gctgatggcc ggtcttgatg gtatcaagaa caagatccat ccgggcgaag ccatggacaa
    28321 aaacctgtat gacctgccgc cagaagaagc gaaagagatc ccacaggttg caggctctct
    28381 ggaagaagca ctgaacgaac tggatctgga ccgcgagttc ctgaaagccg gtggcgtgtt
    28441 cacggatgaa gcgatcgatg cgtacatcgc tctgcgtcgc gaagaagatg accgcgtgcg
    28501 catgactccg catccggtag agtttgagct gtactacagc gtctaatagt tgaagttgta
    28561 ctacccggcg caacaacgcc gggggttcca accaggccca tctggcgtaa ttgttgcagc
    28621 cagtttgaac acggacagcg cgcagaaccc ggagcgtaca ctggtacgtg aggagttcga
    28681 gcactgccca ggttcaaaat ggcaaataaa atagcctgat gggactggtt tttagttgcc
    28741 gtggaaattt cagcccatct ctgcatgggc ttttttctcc gtcaattctc tgatgcttcg
    28801 cgctttttat ccgtaaaaag ctataatgca ctaaaatggt gcaacctgtt caggagactg
    28861 ctttatggca acaggcacgc agcccgatgc tgggcagatc ctcaactcgc tgattaacag
    28921 tattttgtta atcgatgaca acctggcgat ccattacgcc aaccctgccg cgcaacaact
    28981 gctcgcccaa agctcccgca aattgtttgg tacaccgtta ccggaactgt tgagctactt
    29041 ctcattaaat atcgagctga tgcaagaaag tctggaggcg gggcaaggtt ttaccgataa
    29101 cgaagtgacg ctggtcctcg acgggcgctc gcatattctt tctgtgacgg cccagcgtat
    29161 gccggacggc atgatcctgc tggagatggc tccgatggat aaccagcgcc gcttaagtca
    29221 ggaacagcta cagcacgccc agcaggttgc tgcccgtgat ttagtgcgcg gcctggcaca
    29281 tgagattaaa aatccgcttg gcggtttacg tggcgcggcg cagctactca gcaaagcgtt
    29341 acctgaccca tcactactcg aatataccaa agtgattatc gaacaggcgg accggctgcg
    29401 aaatctggtc gaccgtctgt tggggccgca gctgcccggt acgcgcgtta ccgaaagtat
    29461 tcacaaagta gctgaacgcg tggtaacgct ggtgtcgatg gaactgccgg acaacgtgcg
    29521 gttgattcgt gattacgatc ccagcctgcc ggaactggcg cacgacccgg atcaaattga
    29581 acaggtcttg ctgaatattg tgcgcaatgc gctacaggcg ctggggccgg agggtggtga
    29641 aatcattctg cgtacccgca ccgcgtttca actgacctta cacggcgagc gctaccggct
    29701 ggcggcgcgg attgatgtgg aagataacgg gccgggcatt ccgcctcatt tgcaggatac
    29761 gctgttttac ccgatggtca gcggccgcga aggtggcacc gggcttggct tatccatcgc
    29821 tcgtaatttg attgatcagc attcaggcaa aattgaattt accagttggc cagggcatac
    29881 cgagttctcg gtttacctgc ctatcaggaa ataaaggtga cgtttatgca acgagggata
    29941 gtctgggtag tcgatgacga tagttccatc cgttgggtgc ttgaacgtgc gctcgctggg
    30001 gcaggtttaa cctgtacgac gtttgagaac ggcgcagaag tgctggaggc gctggcgagc
    30061 aaaacgccgg atgtgctgct ttcagatatc cgtatgccgg gaatggacgg gctggcgctg
    30121 ctcaagcaga ttaaacagcg ccatccaatg cttccggtca tcattatgac cgcacattcc
    30181 gatctggatg ctgccgtcag cgcctatcaa caaggggcgt ttgattatct gcccaaaccg
    30241 tttgatatcg acgaagcagt ggcgctggtt gagcgcgcta tcagtcatta ccaggaacag
    30301 cagcagccgc gtaatgttca gcttaacggc ccaacgaccg atatcatcgg cgaagcgcca
    30361 gccatgcagg acgtgttccg tattatcggt cggctttcgc gttcttctat tagcgtgctg
    30421 attaacggcg aatccggcac cggtaaagaa ctggtcgctc atgccctgca tcgccacagt
    30481 ccgcgcgcca aagcgccgtt tatcgcgctg aatatggcag ctatcccaaa agatttgatc
    30541 gaatcagaac tgtttggcca cgagaaaggc gcgtttactg gcgcgaatac cattcgtcag
    30601 gggcgttttg aacaggccga tggcggtaca ttattcctcg acgaaattgg tgatatgccg
    30661 ctggatgtgc agacgcgttt gctgcgcgtg ctggcagacg gtcagtttta ccgcgttggc
    30721 ggctatgcgc cggtgaaagt ggatgtgcgg attatcgctg ccactcacca gaatctcgaa
    30781 cagcgagtgc aggaaggtaa gttccgtgag gatctgttcc accgcctgaa cgttatccgc
    30841 gttcatctgc cgccgctgcg cgaacgtcgg gaagatattc cccgtctggc gcgccatttt
    30901 ttacaggttg ccgcgcgcga actgggcgta gaagcgaagt tgctgcatcc ggaaaccgaa
    30961 gctgctctga cgcgtctggc gtggccaggc aacgtacgcc agctggaaaa cacctgccgc
    31021 tggctaacgg tgatggccgc cgggcaggaa gtgttgattc aggatttgcc cggcgaactg
    31081 tttgaatcaa cggttgcgga gagtacttcg caaatgcaac cggacagttg ggcgacactt
    31141 ttagcgcagt gggcagacag agcgctgcgt tccggtcatc aaaatctgct ttccgaagcg
    31201 cagccagagc tggagcggac gttactgacg accgcgttgc gacatacgca ggggcataaa
    31261 caggaagcgg cgcggctact cggctggggc cgcaacaccc tgacgcgtaa gttaaaagag
    31321 ctggggatgg agtgattcac agcttgtgtg taaagattga ttattgagcg caaattgctg
    31381 gtattttacg ctttactgtt ccgataagtt cagtatgatc ttgcccggaa aacggggaga
    31441 gtcattatgc tggaatcaat aattaatctg gtatcgagtg gcgcagttga cagccacaca
    31501 ccgcaaactg ccgttgctgc cgtgctgtgt gccgcaatga ttgggctgtt tagttgagtt
    31561 tcagattgct gatgaagtgc tctttgttca tgccggatgc ggcgtgaacg ccttatccgg
    31621 cctacaaaag caatattttc aacaggtttt gtaggcctga taagcgtagc gcatcaggca
    31681 atcttgttct cagcacattg atggcgcttt cgttttctac tttgtaaacg aagcgccatt
    31741 cactatttaa atcacccgtg agaactgctg catccgcgct ttctggcgca gataggtatc
    31801 aaagcacatg caaatgttgc ggatcagcaa gcgacctttc gccgtcacct gtattccctt
    31861 ctcatccaca tccaccagcc catcttttgc taacggggcg agcagcttga gatcttccgc
    31921 aaagtaatca gcgaagtgca aatcccactg tttctcaata ggggcgtaat ccagacggaa
    31981 gttgcagatg agcgacttaa tcacatcgcg gcgaatacag tcatcacgcg ttagcgcaat
    32041 accacgccac agcgcattgc cttgttcatc cacttgctga tagtactgct tcaactcttt
    32101 ctggttctgc gcgtagcagt cgccaatcat gctgatggcg gaaacgccca tccccagcag
    32161 atcggtatcg ccctgagtgg tgtagccctg gaagttacga tgcagcacgc cttcacgctg
    32221 ggccaccgcc agctcgtcat ccggacgggc aaagtgatcc ataccgataa actgatagcc
    32281 cgattgcgtc aggaaggcga tggtttcctg caggatatcg agtttttgct gcggactcgg
    32341 caggtcagca tctttgattt tgcgctgagc agcaaaaatg gtcggcagat gcgcgtagtt
    32401 aaagacactc agacgatcgg ggttcagctc cgccacacgt ttcagggtaa aggcgaaact
    32461 ctccggcgtc tgtttcggca ggccgtaaat caggtcgatg ttggtggagg taaaaccaat
    32521 ctcacgcgca tggttaagca gtgcaaagat gaactcttca tcctgctcgc ggttaaccag
    32581 acgttgcact tctttgttga agtcctgcac gcccatgctc aggcgattaa agccttcggc
    32641 gcgtaaatga tcgagtacat ccagttcgat ttcccgcgga tcgacttcga tcgaaatctc
    32701 cgcatcggca ttgaactgga agttttcgcg cagcagcttc atcaggcggc tgatttgcgc
    32761 tttattcaga tacgtcggcg ttccgccgcc ccagtgcaat tggctgacgt gacgcccggc
    32821 gaacagcggt gcacgatgga cgatttcttg ctccagcgcg tccagatact gatcggcctt
    32881 gtgctgctgg cgagtaacaa tcttattgca accgcagaag taacaaagct tatggcagaa
    32941 cgggatatgt acgtagagag ataatggacg ctcaggatag cgcgccacgg cttgtaaaaa
    33001 cgcctgttcg ccgaagtctt ctgaaaactc cagcgcggtc gggtacgagg tgtatcgtgg
    33061 cccggaatag ttatatttct ggatcagggc cagatcccag tcgatttgct gtacagacat
    33121 gctcactcct tccgatggcg tctctggcgg ctacggcgta ccggcggatg cccgtttcgg
    33181 gtcatcagcc ggttgcgcag ccacttttgt cgccgcgaca accgctgtag tttaacgaat
    33241 aaccacacca gataacatat aaccggaagg gttatcagca gaacgggaag gcccacggcg
    33301 gcaatccgtt agttgccccg cagcagacgc atcatgtctt cttgcttctc gtcttcttcc
    33361 tcttcttcgt catcgtcata agagaggccg agtttctgca tcaactcatc aatacgatcc
    33421 agtttagcat ccacccagga ttgctcttcg gcactcaggg tttcgcctgc ttccagacgt
    33481 tccagcagcg catccagacg ctcatccgtt tccagtaatt ccaactccgc ctgcggtgaa
    33541 agcataggtt tctcactctt cggtttgtgc tgtttggtga ctttttcagt cacgcccaat
    33601 ggaatagggg ttttactgcc aatacgtgga tcttttggtg cgttctggcc tttgctgcct
    33661 gacgtggtgt ttccgcccgc tgcgcggctg cccggcgcat gaccacgacg ttttttctga
    33721 cgcttgcgat cacgagcttc ctgatccagc tcctcgcgtg tttttcgacg cgcttttgca
    33781 tgacctttgc tgcgtgagtt tgaagatgat ggtttcataa tggttcgtct tttagccgtt
    33841 ttattcagta tagatttgcg gcggaatcta acagaaagca agcaaagaaa aaaggcgaca
    33901 gattactctg tcgccttttt tcctgactca taacccttaa cgggtcttac aatccttgca
    33961 ggccgccaac acaccctgtt tttcctttag ccggaacaat ccgtgttgat tccatttccg
    34021 tttaattacg tctccggacg tttgtcttcc tgacaatcct ctgtcttcgc ctcctggcgc
    34081 tcctgaccct catcctgagt cattgttcct gttagcgtcc tcgctctatg ggtgctactt
    34141 tacgcctttg ctttaaacaa acaatcaacc gccatcagta tttgcacatt ttcagagttt
    34201 tatataaaag caattcactg atttatatag cttatttgac atgcatcgca gaattttctc
    34261 tgctatttcg cataaagact atcggcaatg tctcacaaag cgtatggctt ttacttacaa
    34321 cgctcacgcc gaaaaatccg ccttttatta tggttcaggt ataatcgcca caaagctttg
    34381 actgacggag acgaccgctt tgactaattt gaattatcaa cagacgcatt ttgtgatgag
    34441 tgcgcctgat attcgccacc taccttccga taccggaatt gaagtggctt ttgcaggccg
    34501 ttccaacgca ggtaaatcca gcgcgctgaa cacgctgact aaccagaaaa gcctggctcg
    34561 tacctcaaaa accccagggc gcacccagct tatcaacctg tttgaagtgg ctgacggcaa
    34621 gcgtctggtt gacttgcctg ggtacggtta tgcggaagtc ccggaagaga tgaagcgcaa
    34681 atggcagcgt gcgctcggcg aatacctcga aaaacgtcag agcctgcaag gtctggtggt
    34741 gctaatggat attcgccatc cgctgaaaga tttggatcag cagatgattg agtgggcggt
    34801 agacagcaat atcgccgttc tggtgctgct gaccaaagcg gacaaactgg caagcggcgc
    34861 acgtaaagcg caattgaata tggtgcgtga agctgtactg gcgtttaacg gtgatgtgca
    34921 ggttgaaacg ttttcttcgt tgaagaaaca aggcgtggac aagctgcggc agaaactgga
    34981 tacctggttt agcgagatgc agcctgtaga agaaacgcag gacggcgaat aattttcttg
    35041 ccttaatgct tgtgccggat gtggcgtatc cggcccgtaa attcaataga ttacaattct
    35101 accgttggcc tgataaacat agcgcatcag gcattacgga tcttttcttt cgcccaataa
    35161 aaaacgcccc agtcattact gactggggcg gctaaaatat tcagccaaat ccgattacgt
    35221 gaagtaaaag gtctgaaaga tagaacatct tacctctgta ccctacgcga ctaactttac
    35281 tcttttttgt tcagttcaga aagcactttt tgtaattttt tttcagttgt tgcataggaa
    35341 tttctaatag ccatcacaaa acgtgacagc ttatgttgct tacttacgaa aaaagggcat
    35401 gttcaggcga atcttagtgc gcctgatccc agttttcgcc actccccact tccaccagca
    35461 acggcacatc cagacgggta cagttttcca tcagttgatg aatctgcttc gcgacggcat
    35521 caacatcatc tttatgaact tcaaatacca gttcatcgtg tacctgcatg atcatacgta
    35581 cacgcggttg ctcagcctgt aaccacgcat caacggcaat catcgcccgt ttgataatgt
    35641 cggcggcggt tccctgcatt ggcgcgttaa tggctgcacg ttcagccgct gcacgacgag
    35701 caccattgct ggatttgata tccggcagat acagacggcg tccgtccagc gtttcaacgt
    35761 agccctgctc tttcgcctga gcacgggtgc gttccatata ctccagcacg ccagggtagc
    35821 gttcgaagta aaggtccatg tacttctgcg cttctttacg tggaatgttc aattgccgcg
    35881 ccagaccgaa agcactcatg ccataaatca gaccaaagtt gatcgctttc gcgctacggc
    35941 gttgctcgct ggtgacggtt tccagtggca aaccaaacac ttctgccgcc gttgcccggt
    36001 ggatatcttt tccttccgcg aatgcggtca gcaagccttt gtcacgcgaa agatgcgcca
    36061 taatgcgcag ttcaatctgc gagtagtccg ctgagacaat cacataatcc tctggcgcaa
    36121 taaacgcctg gcggatacga cgaccttctt cgttacgcac cggaatgttt tgcaggttag
    36181 gatcggttga cgataaacgt cccgttgcag ttactgcctg gtgataagag gtatgcacac
    36241 gcccggtttt cgggttgatc atcagcggca gcttgtcggt gtaggtcgat ttcagcttcg
    36301 ccagaccacg atactccaga atcacttttg gcaacggata gtccagcgcc agttcttcca
    36361 gtacctcttc cgacgttgac ggcgcgccac ccggcgtttt cttcagcggt ttaataccct
    36421 gtttttcaaa gagaatggtt tgcaactgct tggtggaaga aaggttaaat tcttcacctg
    36481 caatttcatg cgctttcttt tccagctcag ccagacgcaa ggtgagctct tcagaatgat
    36541 tgtgcagcac tttcggatcg atcttcacac cgttacgttc aatgcgtgaa agcaccggca
    36601 ccagcggcat ttcgatattc tcgaagacgt tcaacggccc tttgtgtttt tgcagatctg
    36661 gccacatttt cagatgcaac tgcaaggtga catcagcatc ttcggcggcg taacgtccgg
    36721 cctcttcgag ggcaatctgg ttaaaggtca gttgattttt gcctttaccc gcaatctctt
    36781 caaaagtgat ggttttgtgc ttcaaccaac gttccgcgag gctgtccata tcgtgacgcc
    36841 cggcaacgct attgagaatg taggactcca gcatggtatc aaacgcaatc ccacgcagct
    36901 caatgccgta attcgccaga ataccgcgat cgtatttcag gttttgcccg accttcagcg
    36961 ccttttcatc ttccagcagc ggttttagca actcgagtgc acgctcgcga gagatttgat
    37021 cgggcgcgtc aagataatca tgagcaaccg gaatatatgc cgctacgcct ggctcgatag
    37081 caaaagaaag cccgaccagg ttagcagaga tgttatcaag gctgtcggtt tcggtatcaa
    37141 atgcaaatac cggcgctttt tccagcttcg caatccacgc tttcagtgtt tcttcatcaa
    37201 ggatggtgac gtagttgtca taagaaatca ccgttgccgt cacttctggc gcttcgtctg
    37261 caacactggt ttcctgtggc ttcgcggctg gttttgcccc tttggcctgt aaccatttgc
    37321 ccgcttcgac atcagcagtc cagcgtttga actcatactt tttgaacagc cccaacaact
    37381 cttccgctgc cggttgctgc acttccagtt gttcacaggt cagctccagt tcaacgtcgg
    37441 ttttaatcgt cgccagctgg tatgagagat aagcaacttc tttgttttgc tcgagcttcg
    37501 ctgccattgt tttcgcgcca cggaagctca acccagcaat tttttctggc tcggcataca
    37561 gcgtatccag tccgccaaga ccttgcagca atgcctgcgc ggttttttca ccgacgcccg
    37621 gtacgccagg aatgttatca gaggagtcac ccatcagcgc caggaaatcg atgatcagtt
    37681 ctggcggcac gccgtactta ttcaccacct cttccggtcc gaggatggta ttcgtcatgg
    37741 tattgataag cgtaatattt ggcgtcacca gctgcgccat atctttatcg ccagtgctga
    37801 tcagcaccgg acgcccggct ttttcggctt cgcgcgccag agtaccgata acgtcgtccg
    37861 cttctacgcc agaaaccgcc agcagcggca gtcccatcgc tttaaccatc gcgtgcaagg
    37921 gttcgatttg tgcacgcaga tcgtccggca ttggcgggcg atgtgatttg taatgttcaa
    37981 acagttcatc acgaaaggtt tttcccttgg cgtcaaagac caccgctgca tgcgtcggtt
    38041 tatattgcat gatcagactg cgcagcatgt tgaggacacc atacatcgca ccggtcggct
    38101 cgcctgcgct gttagtcagc gggggaaacg cgtgatatgc gcgataaaga taagatgaac
    38161 catctacaag gataagtgga ttttggggga tctgaaccat aatgtccgtg cctgtttatc
    38221 agattatggg taaaggatgc cacagacagg atgaaaacat gagtttttca ccgcttttcg
    38281 tgaaaatctt ttgtagatct tctggatcgc tcgcaaatcg tcatgtggat aactttgtgc
    38341 ataaattatt tacatcgctt ttaatttatt atcatgcttt ttacagcatg atgtttgtct
    38401 ttgtttttca tgcagttaaa aataaagcta tccctaataa ttgagataat gggatttttt
    38461 ctaatgtgga tagatatgaa ttatttttct ccttaaggat catccgttat ttgggtcgtt
    38521 ttctgctaaa atctgcgcct tgcgcttctt tagcgcactg tctatggcta accttttgaa
    38581 taaattcatt atgacgagaa tactcgctgc gataaccctt ttgctgagta tcgtattgac
    38641 cattttggtc actatttttt gttccgtccc gatcatcatt gccgggattg taaaactatt
    38701 gctacctgtg ccagtcattt ggcgaaaggt ttcgcgtttt tgtgatttta tgatgtattg
    38761 ctggtgtgaa ggtctggcgg tattactgca tctgaaccca cacctgcaat gggaagttca
    38821 cgggctggaa gggttaagta agaagaactg gtatctgctt atttgtaacc accgtagttg
    38881 ggcagatatt gtcgtactgt gcgtgctgtt tcgtaagcac attccgatga ataaatattt
    38941 cctcaagcaa cagctggcct gggtgccatt tcttggcctg gcgtgctggt cgctggatat
    39001 gccgtttatg aagcgttatt cccgcgctta tttattacgt catccggagc gacgtggtaa
    39061 agatgttgaa accactcggc gttcttgtga aaagtttcgc ctgcatccca ccaccattgt
    39121 caatttcgtt gaaggctccc gtttcacgca agaaaaacat cagcaaaccc actccacttt
    39181 tcaaaacttg ttgccaccaa aggcggcagg cattgcgatg gcgctaaatg tactgggtaa
    39241 acaattcgat aaactgctta atgttacgct gtgttatccg gacaataacc gtcagccgtt
    39301 cttcgatatg ttaagcggta aactgacgcg aattgtcgtt catgtggatt tacagcctat
    39361 tgccgatgag ttacatggcg actacatcaa tgacaaaagc tttaagcgcc attttcagca
    39421 atggctgaac tcactgtggc aggaaaaaga ccgacttctg acgtcgttga tgtcatcaca
    39481 gcggcaggac aagtaaattt tgctcgcctc tgaatgagca gaggcgagtg agtattttaa
    39541 tgaacaaaac gtccggcacg agacataaat tcttcttcgc tcatctcctg tccgttaaaa
    39601 acaactttac ccggtgtata acgcagttgc agcgaggcgg tattgttgtc aatcgtgatt
    39661 aactgaaaca tctgacctaa ggtcatcatc ccgctgattt gtttatcagc ccgcttttgc
    39721 gctttttccg catccattcc ttcagataaa ttaagctgtt ttgcggtttc tgtgacgaca
    39781 ttaagcggta acttcacatc aaaattgagc gatttaatat ctttgtttgt ggatgatgaa
    39841 gatttggctg ggtcggcaat actgatatcc agattggcat ttagttcgcc gagtgcgttc
    39901 ttccatttta ccggttgttt aatggtcggc tcactttttt gtaataacgg taaatactct
    39961 ttgaacaatg cagcattcac ttcttgcaga gcaacttcat cgtttgctaa ctcagggtgt
    40021 gcagcaagct gcttctgcat tgcaatgtta tactggatga taaattggcg caccgctgag
    40081 ggatcaatag attcagcaat caatgaccac tcaccactac cgagttgctg attccctttt
    40141 gttaacttat caagttcgta ggtcagattg acattgatat aatctttgtc tttaacgcga
    40201 tccagcatgg tttttgcaac gaaagcatcg atttgggcaa cgtcttcccc gtgattgata
    40261 atgttaatct gattaaattt cgactcgctt tcaccaaccg gaaatttctt ttcttctaat
    40321 cgcgccagag aatcaatggt aaaacttttt gccgttactt tgttcattgg tgataattgc
    40381 agaacaatat cttcgacttt gccttcaata tccaggttag acaatgaact accatcacca
    40441 ttaaaagtaa actggccctc accaaaagaa aactttccat actctgcagg ggcaacatct
    40501 aatgtcgtag agaatgagcc accaaacgcg aatcgcaatg tcgcttccac gggcgattta
    40561 tttttcgcgg cgataaatag cggttgtgtc agttcattat tcactaagtt cacttttgcc
    40621 gctgccagtg ctgggatgac attaccatgc attaacattg tgatgggcaa cggaccgtgc
    40681 tcaacatcca catcaaatac aactttctgg cctggcttga tattgagatc gggtgcaccg
    40741 ttatcgaatg tcatttgcat ttgaaaacga gaattaaaaa aacctttgtc aaaattttta
    40801 taacttaacg tcatatcgta ggcatgttca cctttctttt tagcatcgtt aaaatcttta
    40861 ataaactttt cgacaccagg ctgaatttgc gtacctgtgt accatgtgcc accaccccag
    40921 attacggcta acgcaacaat aacacctgta gctgacttac gtatcatata aatattccct
    40981 gataaaacag caaaacttta aatatccgct tatgctgaaa taatgtccag cggcaggacg
    41041 cattatcaga atgtatttat atttattaaa taataaaaaa aacccgtgaa tattcacggg
    41101 ccttatgtaa tttacattga attatttttt ctcggacaga tatttcactg tatcagcata
    41161 ctgctgaaca aaaacatcca tattgctagt atccataccc tgcggattca gctgatattt
    41221 accgttaaca aacatcgccg gaacgccacg caattgcacg tcagctgcag ctttttcctg
    41281 ctgagcgacc agagatttga ccacgaagct gttccacgcc gcgtcgtact cttcaccttt
    41341 aatacctgcg ttgataaata catcgcggat atcagatgct gaacgaatgg tctgggtttt
    41401 ctgtacgcct tcaaacagcg gaacagtgac tttgtcttcc acgcccagcg ccatcgccac
    41461 agcccatgcc tgagtcagat ctttgcccag gtcaccaccc atgaagttga cgtggtattt
    41521 agtcatcttc acgccttccg gcagtttttt cttcacatta tcagaaatat gcagaacttc
    41581 ttcaaactga tagcagtgcg gacagaagaa agagaaaaac tccagcactt gcggcgcgcc
    41641 agctaccggt ttttccaggg tagtgtactg tttaccatct tcatactgcg ccgccgatgc
    41701 gctaaacgct aaaactaaac cagccagcgc cagccaaatc tttttcatga tctactctct
    41761 ccgattaata cataggtgtt aattgtaaag ggggttcttg tagaactttt gcctgttcta
    41821 taaaagtcgc cgtttgtcgc agccagtaat cttccccggt taaccacggg aaatttttcg
    41881 ggaacgcggg atcagcccaa cgccgcatta gccaggcaag ataataaacc aaacgcatgg
    41941 cgcgtaaagg ttcaatcagt ccgatttcag cggtgtcgaa ctcgctaaat tcttcataag
    42001 cttcaataat agtttccagt tgcatccgct gctcggcttt atcgccattg agcaacatcc
    42061 acaaatcctg aatagctgga ccattacgtg catcatccag atcaacaaac attggaccat
    42121 cgcgccagag aatattcccg gcgtggcagt ctccatgtag ccgcagaacg gtgaagtctt
    42181 cccgccagtg tgctgtaacg gcggcgatca gctcatctgt cgctttcagg aatgccgctt
    42241 tcaacccgga aggcatcagt gtagcgtcct caaacagctt gcgtggctca atgagatatt
    42301 cattcagacc gatggtcggg cgatggataa aaagctgttt gcgccccgtc tggtgcatac
    42361 gccctaaata acgcccaacc gcttccatct gatcgatatt atcagcttcg aactggcgac
    42421 caccgacgct tggaaaaaca gcgaaataaa atccctgatg attcaataaa gtctgaccgt
    42481 taaaggccac aggtgctgcg accggaactt catcatttac caactgcaac gcaaattgat
    42541 gttcttcgag gatttgatcg gctgtccaac gttcagggcg ataaaatttg acgacaaaac
    42601 gtcgacgatc ttcgtcctga aattgataga cacggttttc atagctgtta agcggggtaa
    42661 gaccggaatc cacccggatc ccttgctcaa atagagcatc catgatggta tccgggtgta
    42721 gtgtctggaa agtaaaagcg ctgttgttca tcccatcatc cggaaaacat tacgaatgat
    42781 gaaggatatc atttcacgat gattttggtg gcaccgctta caagctttta ctctttaatt
    42841 acgccacgag cgcgtaacag cgccgttttg aagtcttctt cataatcttt ctgcaaaccc
    42901 gggatcaccg catctttcgc ggaatcacgc attttcaggt gatagatcag aatgtcatcc
    42961 gtcaaatccg ccagttcgcc gtcaaaacct gactctttcg ccagtttctg caaaaattgc
    43021 aacaggttaa ggtctggctc tttttgccag gctggctgga ggagttcaat aacttcattc
    43081 agacgtttac atttcatagt aatgctcctt actcttgaga cagacacgtt agcagggtca
    43141 atcccacaat aaaagaggcg atatcggtga atctgatgac gacgataaca ggcgttgtgc
    43201 tggcaggcgg taaagccaga cgaatgggcg gcgtagataa aggattgctt gaattaaacg
    43261 gcaaaccatt atggcaacat gtcgctgacg cgcttatgac gcagctctct cacgtcgtgg
    43321 ttaatgccaa tcgccatcag gaaatctatc aggcaagcgg tctgaaagtg attgaagatt
    43381 cactggcgga ttacccaggc cctctggcag gaatgctttc agtaatgcag caggaagcag
    43441 gcgagtggtt tttgttttgt ccgtgcgata ctccttatat tcccgaggat ttagcagccc
    43501 ggcttaatca tcagcgcaaa gatgcgcctg tcgtgtgggt ccacgacggt gaacgcgatc
    43561 acccgactat tgctctggta aaccgcgcaa ttgagccttt attactggaa tatctgcaag
    43621 caggagaacg ccgggtaatg gcatttatgc gtctggctgg cggtcatgcg gttgatttca
    43681 gcgataataa agatgcattt attaacgtga atacgccaga ggagcttgcc cgatggcagg
    43741 aaaaacgatg ataccgttac tcgcctttgc cgcgtggagt ggtaccggaa aaactacgct
    43801 tttgaaacaa ctgatcccgg cattatgcgc cagagggatc cgtccagggc tgattaagca
    43861 tacgcaccat gatatggatg ttgataagcc aggcaaagat agctatgagc tgcgcaaggc
    43921 tggcgcggca caaaccatcg ttgccagcca gcaacgatgg gccttgatga cggaaacacc
    43981 agacgaagaa gagctggatc tacagtttct cgcaagtcga atggatacct caaagctgga
    44041 tttgattctg gtcgaagggt ttaagcatga agagatcgca aagattgtgc tgtttcgcga
    44101 tggagccgga catcgacctg aagaattagt gatagacagg catgttattg ctgtagccag
    44161 tgatgtgccg cttaatcttg atgtcgcatt actggatatt aatgatgttg aggggctggc
    44221 tgattttgtg gtggagtgga tgcaaaagca gaacggataa gcaagagtag gctggataag
    44281 acgcgccagc gtcgcatccg gcattttttt ctgcgccaat gcaaaaaggc catcctgacg
    44341 gatggccttc tgcttaattt gatgcctggc agttccctac tctcgcatgg ggagacccca
    44401 cactaccatc ggcgctacgg cgtttcactt ctgagttcgg catggggtca ggtgggacca
    44461 ccgcgctaag gccgccaggc aaattctgtt ttatcagacc gcttctgcgt tctgatttaa
    44521 tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca ccttcggcgt tgtaaggtta
    44581 agcctcacgg ttcattagta ccggttagct caacgcatcg ctgcgcttac acatccggcc
    44641 tatcaacgtc gtcgtcttca acgttccttc aggactctca aggagtcagg gagaactcat
    44701 ctcggggcaa gtttcgtgct tagatgcttt cagcacttat ctcttccgca tttagctacc
    44761 gggcagtgcc attggcatga caacccgaac accagtgatg cgtccactcc ggtcctctcg
    44821 tactaggagc agcccccctc aattctccag cgcccacggc agatagggac cgaactgtct
    44881 cacgacgttc taaacccagc tcgcgtacca ctttaaatgg cgaacagcca tacccttggg
    44941 acctacttca gccccaggat gtgatgagcc gacatcgagg tgccaaacac cgccgtcgat
    45001 atgaactctt gggcggtatc agcctgttat ccccggagta ccttttatcc gttgagcgat
    45061 ggcccttcca ttcagaacca ccggatcact atgacctgct ttcgcacctg ctcgcgccgt
    45121 cacgctcgca gtcaagctgg cttatgccat tgcactaacc tcctgatgtc cgaccaggat
    45181 tagccaacct tcgtgctcct ccgttactct ttaggaggag accgccccag tcaaactacc
    45241 cacca
//
LOCUS       DAPHDW010000021        44720 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.021, whole genome shotgun sequence.
ACCESSION   DAPHDW010000021 DAPHDW010000000
VERSION     DAPHDW010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44720)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44720)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44720
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.021"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(339..881)
                     /locus_tag="RH057_001123"
     CDS             complement(339..881)
                     /locus_tag="RH057_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3127529.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(904..1929)
                     /gene="nagZ"
                     /locus_tag="RH057_001124"
     CDS             complement(904..1929)
                     /gene="nagZ"
                     /locus_tag="RH057_001124"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HDV3127530.1"
                     /translation="MGPVMLDVKGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALSGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKAGH"
     gene            complement(1940..2764)
                     /gene="thiK"
                     /locus_tag="RH057_001125"
     CDS             complement(1940..2764)
                     /gene="thiK"
                     /locus_tag="RH057_001125"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HDV3127531.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKAGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(2745..3386)
                     /gene="lpoB"
                     /locus_tag="RH057_001126"
     CDS             complement(2745..3386)
                     /gene="lpoB"
                     /locus_tag="RH057_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HDV3127532.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDQTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSSASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(3400..3777)
                     /locus_tag="RH057_001127"
     CDS             complement(3400..3777)
                     /locus_tag="RH057_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HDV3127533.1"
                     /translation="MRKGCFGLVSLALLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTLYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(3780..4139)
                     /gene="hinT"
                     /locus_tag="RH057_001128"
     CDS             complement(3780..4139)
                     /gene="hinT"
                     /locus_tag="RH057_001128"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HDV3127534.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            4473..6662
                     /gene="fhuE"
                     /locus_tag="RH057_001129"
     CDS             4473..6662
                     /gene="fhuE"
                     /locus_tag="RH057_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
                     /protein_id="HDV3127535.1"
                     /translation="MLSTQFNRDNQYQAITKPSLLAGCIALALLPSAAFAAPATEETV
                     IVEGSATAPDDGENDYSVTSTSAGTKMQMTQRDIPQSVTIVSQQRMEDQQLQTLGEVM
                     ENTQGISKSQADSDRALYYTRGFQIDNYMVDGIPTYFESRWNLGDALSDMALFERVEV
                     VRGATGLMTGTGNPSAAINMVRKHATSREFKGDVSAEYGSWNKERYVADLQSPLTEDG
                     KIRARIVGGYQNNDSWLDRYNSEKTFFSGIVDADLGDLTTLSAGYEYQRIDVNSPTWG
                     GLPRWNTDGSSNSYDRARSTAPDWAYNDKEINKVFMTLKQRFADTWQATLNATHSEVE
                     FDSKMMYVDAYVNKADGMLVGPYSNYGPGFDYVGGTGWNSGKRKVDALDLFADGSYEL
                     FGRQHNLMFGGSYSKQNNRYFSSWTNIFPDEIGSFYNFNGNFPQTDWSPQSLAQDDTT
                     HMKSLYAATRVTLADPLHLILGARYTNWRVDTLTYSMEKNHTTPYAGLVFDINDNWST
                     YASYTSIFQPQNDRDSSGKYLAPITGNNYELGLKSDWMNSRLTTTLAIFRIEQDNVAQ
                     STGTPIPGSNGETAYKAVDGTVSKGVEFELNGAITDNWQLTFGATRYIAEDNEGNAVN
                     PNLPRTTVKMFTSYRLPVMPELTVGGGVNWQNRVYTDTVTPYGTFRAEQGSYALVDLF
                     TRYQVTKNFSLQGNVNNLFDKTYDTNVEGSIVYGAPRNFSITGTYQF"
     gene            complement(6722..8155)
                     /gene="ptsG"
                     /locus_tag="RH057_001130"
     CDS             complement(6722..8155)
                     /gene="ptsG"
                     /locus_tag="RH057_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HDV3127536.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFSFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLFPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(8450..9247)
                     /locus_tag="RH057_001131"
     CDS             complement(8450..9247)
                     /locus_tag="RH057_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3127537.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(9258..10262)
                     /gene="holB"
                     /locus_tag="RH057_001132"
     CDS             complement(9258..10262)
                     /gene="holB"
                     /locus_tag="RH057_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HDV3127538.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNALGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSRLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(10259..10900)
                     /locus_tag="RH057_001133"
     CDS             complement(10259..10900)
                     /locus_tag="RH057_001133"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HDV3127539.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(10890..11912)
                     /gene="yceG"
                     /locus_tag="RH057_001134"
     CDS             complement(10890..11912)
                     /gene="yceG"
                     /locus_tag="RH057_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HDV3127540.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTWMYTANTTDVALLKRAHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(11915..12724)
                     /gene="pabC"
                     /locus_tag="RH057_001135"
     CDS             complement(11915..12724)
                     /gene="pabC"
                     /locus_tag="RH057_001135"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HDV3127541.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIQ
                     RLQDACQRLMIFCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTVYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVSFSSATLYEYLAPLCERPN"
     gene            complement(12844..14085)
                     /gene="fabF"
                     /locus_tag="RH057_001136"
     CDS             complement(12844..14085)
                     /gene="fabF"
                     /locus_tag="RH057_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HDV3127542.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(14173..14409)
                     /gene="acpP"
                     /locus_tag="RH057_001137"
     CDS             complement(14173..14409)
                     /gene="acpP"
                     /locus_tag="RH057_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3127543.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(14620..15354)
                     /gene="fabG"
                     /locus_tag="RH057_001138"
     CDS             complement(14620..15354)
                     /gene="fabG"
                     /locus_tag="RH057_001138"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV3127544.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(15367..16296)
                     /gene="fabD"
                     /locus_tag="RH057_001139"
     CDS             complement(15367..16296)
                     /gene="fabD"
                     /locus_tag="RH057_001139"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV3127545.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGPAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDASIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVPVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(16312..17265)
                     /gene="fabH"
                     /locus_tag="RH057_001140"
     CDS             complement(16312..17265)
                     /gene="fabH"
                     /locus_tag="RH057_001140"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HDV3127546.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLGIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(17333..18403)
                     /gene="plsX"
                     /locus_tag="RH057_001141"
     CDS             complement(17333..18403)
                     /gene="plsX"
                     /locus_tag="RH057_001141"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HDV3127547.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(18484..18657)
                     /gene="rpmF"
                     /locus_tag="RH057_001142"
     CDS             complement(18484..18657)
                     /gene="rpmF"
                     /locus_tag="RH057_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HDV3127548.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(18709..19230)
                     /gene="yceD"
                     /locus_tag="RH057_001143"
     CDS             complement(18709..19230)
                     /gene="yceD"
                     /locus_tag="RH057_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HDV3127549.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEAQK
                     PNPFAVLASLKRK"
     gene            19429..20013
                     /gene="yceF"
                     /locus_tag="RH057_001144"
     CDS             19429..20013
                     /gene="yceF"
                     /locus_tag="RH057_001144"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HDV3127550.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKAQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(20125..21084)
                     /gene="rluC"
                     /locus_tag="RH057_001145"
     CDS             complement(20125..21084)
                     /gene="rluC"
                     /locus_tag="RH057_001145"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HDV3127551.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            21657..24842
                     /gene="rne"
                     /locus_tag="RH057_001146"
     CDS             21657..24842
                     /gene="rne"
                     /locus_tag="RH057_001146"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HDV3127552.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAAPVVAPAPKAAPATPATPA
                     QPGLLSRFFGALKALFSGSEETKPTEQPAPKAEAKPERQQDRRKPRQNNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQAPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPVVEPQEETADIEEVAETAEVVVAEPE
                     VVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDWQR
                     PTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(25039..25992)
                     /gene="flgL"
                     /locus_tag="RH057_001147"
     CDS             complement(25039..25992)
                     /gene="flgL"
                     /locus_tag="RH057_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
                     /protein_id="HDV3127553.1"
                     /translation="MRFSTQMMYQQNMRGITNSQAEWMKYGEQMSTGKRVVNPSDDPI
                     AASQAVVLSQAQAQNSQYTLARTFATQKVSLEESVLSQVTTAIQNAQEKIVYASNGTL
                     SDDDRASLATDIQGLRDQLLNLANTTDGNGRYIFAGYKTETAPFSEEKGKYVGGAESI
                     RQQVDASRSMVIGHTGDKIFDSITSNAVAEPDGSASETNLFAMLDSAIAALKTPVADS
                     EADKEIAAAALDKTNRGLKNSLNNVLTVRAELGTQLNELESLDSLGSDRALGQTQQMS
                     DLVDVDWNATISSYIMQQTALQASYKAFTDMQGLSLFQLNK"
     gene            complement(26004..27647)
                     /gene="flgK"
                     /locus_tag="RH057_001148"
     CDS             complement(26004..27647)
                     /gene="flgK"
                     /locus_tag="RH057_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
                     /protein_id="HDV3127554.1"
                     /translation="MSSLINNAMSGLNAAQAALNTASNNISSYNVAGYTRQTTIMAQA
                     NSTLGAGGWVGNGVNVSGVQREYDAFITNQLRAAQTQSSGLTARYEQMSKIDNMLSTS
                     TSSLATQMQDFFTSLQTLVSNAEDPAARQALIGKSEGLVNQFKTTDQYLRDQDKQVNI
                     AIGASVDQINNYAKQIASLNDQISRLTGVGAGASPNNLLDQRDQLVSELNQIVGVEVS
                     VQDGGTYNITMANGYSLVQGSTARQLAAVPSSADPSRTTVAYVDGTAGNIEIPEKLLN
                     TGSLGGILTFRSQDLDQTRNTLGQLALAFAEAFNTQHKAGFDANGDAGEDFFAIGKPA
                     ILQNTKNKGDVAIGATVTDASGVLATDYKISFDNNQWQVTRLASNTTFTVTPDANGKV
                     AFDGLELTFTGTPAVNDSFTLKPVSDAIVNMDVLITDEAKIAMASEEDAGDSDNRNGQ
                     ALLDLQSNSKTVGGAKSFNDAYASLVSDIGNKTATLKTSSTTQGNVVTQLSNQQQSIS
                     GVNLDEEYGNLQRFQQYYLANAQVLQTANAIFDALINIR"
     gene            complement(27713..28654)
                     /gene="flgJ"
                     /locus_tag="RH057_001149"
     CDS             complement(27713..28654)
                     /gene="flgJ"
                     /locus_tag="RH057_001149"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HDV3127555.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKAGEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEEPTPAAPMKFPLETVVRYQNQALSQLVQKAVPRNYDDSLPGDSKAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGPVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQALQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(28654..29751)
                     /gene="flgI"
                     /locus_tag="RH057_001150"
     CDS             complement(28654..29751)
                     /gene="flgI"
                     /locus_tag="RH057_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HDV3127556.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(29763..30461)
                     /gene="flgH"
                     /locus_tag="RH057_001151"
     CDS             complement(29763..30461)
                     /gene="flgH"
                     /locus_tag="RH057_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HDV3127557.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSYANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFNGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(30514..31296)
                     /gene="flgG"
                     /locus_tag="RH057_001152"
     CDS             complement(30514..31296)
                     /gene="flgG"
                     /locus_tag="RH057_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HDV3127558.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYTRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(31434..32189)
                     /gene="flgF"
                     /locus_tag="RH057_001153"
     CDS             complement(31434..32189)
                     /gene="flgF"
                     /locus_tag="RH057_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HDV3127559.1"
                     /translation="MDHAIYTAMGAASQTLNQQAVTASNLANASTPGFRAQLNALRAV
                     PVEGLSLPTRTLVTASTPGADMTPGKMDYTSRPLDVALQQDGWLAVQSADGSEGYTRN
                     GSIQVDPTGQLTIQGHPVIGEAGPIAVPEGAEITIAADGTISALNPGDPANTVAPVGR
                     LKLVKATGSEVQRGDDGIFRLSAESQATRGPILQADPTLRVMSGVLEGSNVNAVAAMS
                     DMIASARRFEMQMKVISSVDDNAGRANQLLSMS"
     gene            complement(32209..33414)
                     /gene="flgE"
                     /locus_tag="RH057_001154"
     CDS             complement(32209..33414)
                     /gene="flgE"
                     /locus_tag="RH057_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HDV3127560.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLVNMQGLQLTGYPATGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     TGTAEPAMKLVFNANGVLTSNPTENITTGAINGADPATFSLSFLNSMQQNTGANNIVA
                     TTQNGYKPGDLVSYQINDDGTVVGNYSNEQTQLLGQIVLANFANNEGLASEGDNVWSA
                     TQSSGVALLGTAGTGNFGTLTNGALEASNVDLSKELVNMIVAQRNYQSNAQTIKTQDQ
                     ILNTLVNLR"
     gene            complement(33439..34134)
                     /gene="flgD"
                     /locus_tag="RH057_001155"
     CDS             complement(33439..34134)
                     /gene="flgD"
                     /locus_tag="RH057_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HDV3127561.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGTLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(34146..34550)
                     /gene="flgC"
                     /locus_tag="RH057_001156"
     CDS             complement(34146..34550)
                     /gene="flgC"
                     /locus_tag="RH057_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
                     /protein_id="HDV3127562.1"
                     /translation="MALLNIFDIAGSALTAQSQRLNVAASNLANADSVTGPDGQPYRA
                     KQVVFQVNAAPGAATGGVKVADVIESQAPDKLVYEPGNPLADAKGYVKMPNVDVVGEM
                     VNTMSASRSYQANVEVLNTVKSMMLKTLTLGQ"
     gene            complement(34554..34970)
                     /gene="flgB"
                     /locus_tag="RH057_001157"
     CDS             complement(34554..34970)
                     /gene="flgB"
                     /locus_tag="RH057_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HDV3127563.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            35125..35784
                     /gene="flgA"
                     /locus_tag="RH057_001158"
     CDS             35125..35784
                     /gene="flgA"
                     /locus_tag="RH057_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HDV3127564.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAISLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSGQVVSGVVDADGNILIN
                     L"
     gene            35860..36153
                     /gene="flgM"
                     /locus_tag="RH057_001159"
     CDS             35860..36153
                     /gene="flgM"
                     /locus_tag="RH057_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HDV3127565.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            36158..36574
                     /gene="flgN"
                     /locus_tag="RH057_001160"
     CDS             36158..36574
                     /gene="flgN"
                     /locus_tag="RH057_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HDV3127566.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTTHRGGKKISI"
     gene            complement(36614..38149)
                     /gene="murJ"
                     /locus_tag="RH057_001161"
     CDS             complement(36614..38149)
                     /gene="murJ"
                     /locus_tag="RH057_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HDV3127567.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(38259..39182)
                     /locus_tag="RH057_001162"
     CDS             complement(38259..39182)
                     /locus_tag="RH057_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3127568.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWMLQGAWSPTRAKALP
                     ICESWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLRD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVHKPIPGWQSTLEQRGFVGCARHFIEC
                     VQNQTVPQTAGEQAVLAQRIVDKIWRDAMSE"
     gene            complement(39184..39831)
                     /locus_tag="RH057_001163"
     CDS             complement(39184..39831)
                     /locus_tag="RH057_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HDV3127569.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVELTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTDMSNAVDGDLQARVEALEIEVAELKQRLDSLLVHLGD"
     gene            complement(39842..40426)
                     /gene="rimJ"
                     /locus_tag="RH057_001164"
     CDS             complement(39842..40426)
                     /gene="rimJ"
                     /locus_tag="RH057_001164"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HDV3127570.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            40662..41870
                     /gene="mdtH"
                     /locus_tag="RH057_001165"
     CDS             40662..41870
                     /gene="mdtH"
                     /locus_tag="RH057_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HDV3127571.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSAGAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            41934..42581
                     /gene="grxB"
                     /locus_tag="RH057_001166"
     CDS             41934..42581
                     /gene="grxB"
                     /locus_tag="RH057_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HDV3127572.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYANKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVADYRDNMAKQTQINLLSSMAI"
     gene            42715..43275
                     /locus_tag="RH057_001167"
     CDS             42715..43275
                     /locus_tag="RH057_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3127573.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            43381..44427
                     /gene="pyrC"
                     /locus_tag="RH057_001168"
     CDS             43381..44427
                     /gene="pyrC"
                     /locus_tag="RH057_001168"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HDV3127574.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHNFTPLMTCYLTDSLDPNELERGFNEGVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            44501..>44720
                     /gene="dinI"
                     /locus_tag="RH057_001169"
     CDS             44501..>44720
                     /gene="dinI"
                     /locus_tag="RH057_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HDV3127575.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWES"
BASE COUNT        10814 a        12100 c        11141 g        10665 t
ORIGIN      
        1 gttacagcaa tgaaaatgtt cttcgctatt gagaataaca tgaatggtgc attggtcata
       61 ccaaaattga tgttaatcaa gttttgttgc tgaaaagata ggcgacttta ccgggtgaga
      121 tcaaaaaaat agagtttatt gtttattatt tgagcaaatt cggaagagta atatagggtg
      181 aagcgtatat cgagggcgtt tggtcgtcct gataagatgc gcagcatcgc atcaggcatt
      241 gatgctcgac tgtcggatgc ggcgtggacg ccttatccga cttacagaat tcggagcgca
      301 tcaggtaccg gcaaggttac ctgatgcgac gagggcattt acccgagggt tttgaacgct
      361 ttaatgcgct gtaaatgcgg ggagatattc ttgaatttgt gcgtctgctc ttcgtcccag
      421 acaatctcgt aataatgatg caactcttca gatgtccgct ggctgttaag tgcttcatca
      481 ttacgcgaca aaataaccag acaacgatcg cgattcttct cgcggaagtt ggtcacacac
      541 ttagtggcaa tatcggcata ctcttccggg cgatcaatct tgccttccat gttctcataa
      601 gggaacaaat tagggttgaa gatcacctgg cggatgtcgc aaagaaaacc aatccgttcc
      661 gcccagtatc cgcccaggcc aacgccgcaa attagcggac gctcgtcaac gttcagttgc
      721 aacattttgt ccacttcttt aagcagatgc tgcatatcat gtttcggatg ccgcgtactg
      781 tagcttatca agcgtacatc cgggtcaata aactgcaatt gtaagacttt ctcgtggtta
      841 cccggactgt tagagtcaaa accgtgtaaa tagataatca tcgcttcctc acataagcca
      901 gggttagtga cctgctttct cttcctgcca gcgttcgtgt aactgattca gacgggtgct
      961 gatcgctttc cagcgagccg agtccatcag ttcctgtcgc gaaaatgaac ctttatgata
     1021 caaacgtgta acacgttctg ccttgatcgg tgacagatta tctaacacgc tgacggcccc
     1081 tttacgatta ttgcagacca ggatcatatc gcaacccgca tccagtgatg cctgcccgcg
     1141 ttcggcataa ctgcccataa tcgcggcacc ttccatcgat aaatcgtcag agaaaatcac
     1201 gccgtcaaaa cccagttcct gacgcaaaac ggttttcagc cagtagggag aaccgctcgc
     1261 cggacgcgga tcaacatcac tgtagatcac atgcgcaggc ataatggcgt cgagtttatt
     1321 ttcgcggatt aaggaactga agaccgacat atctttagcg cgaatctccg cttgtggacg
     1381 tggatcgcac ggtgtttctt tgtgtgagtc tgccgttact gcaccgtgtc ctgggaagtg
     1441 tttcccggtc gttttcattc cggcttcatg cataccatca ataaaccggc tggcaattgc
     1501 cagggctttt tgtggatcgg catgataaga acgctcgcca atcgccgcgc tgatatgccc
     1561 gacatccagc actggcgcaa agctgatatc aatatccata gcgatcattt cgctggccat
     1621 cagccaaccc gcctcttgcg ccagtttgcc gccctcttcc attcctgaca gcgcagcgaa
     1681 tgattgcgcc gctggcaagc gggtaaaacc ttcacgaaaa cgctgcacgc gtccaccttc
     1741 ctgatctacc gccaccacca gatgattgcg cgaagctgcg cggatctggc gcaccagttc
     1801 acgtaactgg gcaggatcat gatagttacg cgtaaagaga atcagccctc ccaccagcgg
     1861 atgcgcaagt atttcacgct cttccgcgtc cagttcgtaa cctttgacat ccaacattac
     1921 tggacccaca ctgctctcct tattgtttta ttaatagctg ccgccaggtg tcatcggcca
     1981 gcctgataaa ttgttgatcg ccggtttgtc gccagcggta ctcaaaccac cctgctttga
     2041 gcatcagcag ccagggaaac catcgcctga cctgacgcca taattgcgcc ggataaatct
     2101 tcgcgcgagt ggcatagtca ttgaccaatt gccggtgctg ttcagtattt tccacccaca
     2161 ccgccgccag ttccagcgcg atatcaccat ctccggcata ctcccagtcg atgagtttta
     2221 accctgacgc gctatgcact aaatttccgg cgtggacatc catatgcaat ggacttaagc
     2281 gtaaaggccg tggttcccgc gctttgcgca gacgttttaa cattcgcagc caacccactg
     2341 tccgccgcgc cggatcgctt tgctgccagt acagttccag taacggcaac agcgttattc
     2401 gccagccaaa acgtggttgt tgatgtagat aatacagcaa gcctgccagt tcgttggtat
     2461 ccggcaaata cgtttttacc gcgccgggca gatagtcgac taccatccag tcacggagat
     2521 ataaatgcgg cttcggtgca atgcttgcgg gtagttgtga taaagcccga tactggcgca
     2581 agaacgcgga ctgcggcgca tcaggatcgt gcggctgacg cacaacaaaa cgctggccct
     2641 gatgttcaat gagaaaactc ccgccactaa gcccactatt aaacgtcgtg acgggatgaa
     2701 actgcgggaa aaagcgcgac agtaattcgt cgcgcgtgat gggattattg ctgcgaaacg
     2761 gcacctttac ctgaccagat aatttcgccc gtctgcacca gcatcagctg catttgtagg
     2821 gtcggagcgt taacgttgcc agaggcgctg gagtacagca cgtaatgagc gccgacattg
     2881 cgggcaatgc ctatggcttt actacgggtg cctaaactgt cctgcggcga caaacctaac
     2941 tgttgcttag ccatcgacag ctgctgggcg gaaaccaggg taaatttccc gttattagcc
     3001 agcgcatttc gcagcgtttc ggtcgcttct gcggcattca gcgaaccgtt agtacggttg
     3061 ttaacgctat caaccagcag gacgctaccc gcagtcaccc cgtcagcccc aagcatctta
     3121 ctgaccatcg gctgcattgc gccattccag tcataatggc gaatatgcgg cgcaggcggt
     3181 gccgtttgat cttcgtgctc aattgggcct ggctgctgcg ggatcgtcgg caccgagggc
     3241 actgtgggga caggctgttg tggctcggct ggttgttccg gcgctggttt cacttcttct
     3301 accggtgcag gttcacgttg ccccacacat ccggcgagaa acatcgccag cgcggtaatc
     3361 aaggcgtagc gactcatttt tgtcatcaag attcacccct tacaaatata gataaagtct
     3421 gactttgtgc gcccccagaa aattggcgct gccgtacagc gttaccgccg aatgtgcggg
     3481 aatggtaacg ctgcgtggcc tttccagagg atgcatctcc agccctctgg cgtcatacca
     3541 gtaaaaacga taatgaacgg taacgggttc ttgcctttcg ttataaagcg ttgaggatgc
     3601 tgaaggttga atatcagacg tcgaaaggac gggcttttct gcactgatgc ccgcagccaa
     3661 taaagatgac tccatcacca gcgattgctc atcattcacc ggaatttccg gatgtgaacg
     3721 acagcccacc agcagtaaca acgccagaga caccagccca aagcatcctt ttctcatcgt
     3781 tacagacctt tatgcgccag cattggtccc agcggacggc cacccaacaa gtgcatatgg
     3841 atgtggtaaa cctcttgtcc gccatggcgg ttggtattca tgatcagacg atagccatct
     3901 tcggcaatac cttcttgctc agcaattttt gccgctacgg tgatcatgcg tcccagcgcc
     3961 tgctcatgct cagctgagac gtcgttcaca gtcggaatga ggatattcgg aatgatcaga
     4021 atatgcgttg gcgcttgcgg cgaaatatcg cgaaacgccg ttaccagatc atcctggtag
     4081 acgatatcgg aggggatctc acgacgaata attttgctga atatagtttc ttctgccacg
     4141 acattttcct ttttcaaata gccatgcgtt gcgccatgct acgccgcgcc acactgcgag
     4201 tatagagtat ggtcgagtta aacctgctct ttcaacctta actcacgatt tcttaagcaa
     4261 aaaatcgttt tgctgccgta tatatcgcca ttattcccat ttctgcctgc gaatgcgctg
     4321 aaaagacatt ctctgtttgg tttacacgac aaatatgaat gcgtatattt ctcatttgca
     4381 tttacaaaca aaattattcg cacactaaat ataatcgctc atcaccctcc tccggcatga
     4441 gcctgacgac aacataaacc aagagatttc aaatgctttc aacacaattt aacagggata
     4501 atcaatatca agccatcacc aaaccgtcac tacttgccgg ttgcatagca ctggcactat
     4561 taccttctgc cgcttttgct gcaccagcca ctgaagaaac ggtgattgtt gagggttcag
     4621 ccacagctcc agatgatggc gaaaatgatt acagcgtaac gtctacctct gcgggtacca
     4681 aaatgcagat gactcaacgt gatattcctc agtcggtcac tattgttagc cagcagcgga
     4741 tggaagatca gcagttacaa acgctgggcg aagtgatgga aaacacgcag gggatcagca
     4801 aaagtcaggc ggattccgat cgtgctcttt attatacccg cggattccag atcgataact
     4861 atatggttga tggtatcccc acctattttg aatcgcgctg gaatctgggc gacgcacttt
     4921 ctgatatggc actctttgaa cgcgtagaag tagtgcgtgg cgcgacagga ctcatgaccg
     4981 ggacgggtaa tccatctgcg gcaattaata tggttcgaaa acacgcgacc agtcgtgaat
     5041 ttaaaggcga tgtctcggcg gaatacggta gctggaacaa agaacggtat gtggcggatt
     5101 tacaaagccc actcaccgaa gacggtaaaa tccgcgcgcg aattgtcggc ggctaccaga
     5161 ataacgactc atggctggac cgctacaaca gtgaaaagac cttcttctcc ggcattgtcg
     5221 atgctgattt aggcgatctt acgacgcttt cagccggtta cgaatatcag cgcattgatg
     5281 ttaatagccc aacctggggc ggtttaccgc gctggaatac tgatggcagc agcaacagtt
     5341 acgatcgcgc acgcagtacc gcacctgact gggcgtacaa cgataaagag atcaacaagg
     5401 tctttatgac cctgaagcag cggtttgctg atacctggca agcgacgctg aatgccaccc
     5461 actctgaagt cgaatttgac agcaaaatga tgtatgtcga tgcctatgta aacaaagcgg
     5521 atggtatgct ggttgggcca tacagtaatt atggacctgg ctttgattat gtcggcggca
     5581 ccggttggaa cagtggcaaa cgtaaagttg atgcgctgga tttgttcgct gacggtagtt
     5641 atgaattgtt tggtcgtcag cacaatctaa tgtttggtgg cagttacagc aaacaaaaca
     5701 atcgttactt cagttcatgg accaacatct tcccggatga aattggcagt ttctacaact
     5761 ttaatggcaa tttcccacaa accgactggt caccacagag cctggcgcag gacgatacca
     5821 cacatatgaa atcgttatat gctgccactc gtgtcaccct tgccgatccg ctgcatctga
     5881 tcctcggcgc acgttatacc aactggcggg ttgatacgct gacttacagc atggagaaaa
     5941 accacaccac gccttacgct ggtctggtgt ttgacatcaa tgacaactgg tcgacctacg
     6001 ccagctatac ctctattttc cagccgcaaa atgatcgtga cagttcaggc aaatatctgg
     6061 ctccaatcac cggtaacaac tacgagctgg gtctgaaatc ggactggatg aatagccgtc
     6121 tgaccaccac gttagccatc ttccgtattg agcaggataa tgtcgctcag tccaccggta
     6181 cacctatccc cggcagcaac ggcgaaaccg cctataaagc agtggatggg acagtcagta
     6241 aaggggtgga atttgaactc aacggcgcaa ttaccgacaa ctggcagctg acatttggcg
     6301 caacgcgcta tattgcagag gataacgaag gaaacgccgt taatcctaat ctgccacgca
     6361 ccacggttaa aatgttcacc agctatcggt tgcctgtcat gccagagttg acagtcggcg
     6421 gtggtgttaa ctggcaaaat cgcgtgtata ccgacaccgt gacaccgtat ggcaccttcc
     6481 gcgccgagca aggtagctac gcgctggtgg atctcttcac ccgctaccag gtgacgaaaa
     6541 acttctcgtt acaggggaac gtcaataacc tgttcgacaa aacctacgat accaacgtgg
     6601 aaggttctat cgtctacggc gcaccgcgta atttcagcat taccggcacg tatcaattct
     6661 gaataacacc tgtaaaaaag gcagccatct ggctgcctta gtctccccaa cgtcttacag
     6721 attagtggtt acggatgtac tcatccatct cggttttcag gttgtcggat ttagtaccga
     6781 aaatcgcctg aacaccagaa ccagcaacca ctacgcccgc tgcgcccagt ttcttcaggc
     6841 cagcctgatc cactttagac acatcagcaa cgctgacgcg cagacgggta atacatgcgt
     6901 cgaggttagt aatgttttct ttaccaccaa atgcagcaac cagagccggt gccatttcgc
     6961 tggtacctgt cgcttttgca tcttcagtcg cgtcttcacg acccggcgtt ttcagatcca
     7021 gtgctttaat cagcacgcgg aagatggtgt agtaaacaat cgcataaccg ataccgacga
     7081 tcgggaacag ccacagtttg ctgctgttac cagacagaac gatgaagtcg atcagaccgt
     7141 gcgagaacga cgtaccgtca cgcatcccca gaagaataca gattgggaat gccaggcctg
     7201 ccagaatcgc gtggatgatg tacaggatcg gcgcaacgaa catgaaggag aactcgatcg
     7261 gctcggtgat accggtcagg aacgaggtca gcgccgcgga gatcataata ccgcccactt
     7321 tcgcgcggtt ttctggttta gcagagtgcc agatagcaat tgcggcagct ggcagaccgt
     7381 acattttgaa caggaagcca ccagacagtt tacccgcagt cgggtcaccc gccatataac
     7441 gcggaatgtc gccgtggaaa acctgacctg ctgcgttggt gtattcacca atctgcatct
     7501 ggaaaggtac gttccagatg tggtgcagac caaacggtac caggcaacgt tcgatgaaac
     7561 cgtaaatgcc aaacgcaact accgggttct ggtaagcagc ccactgagag aaggtctgga
     7621 ttgcagaacc aatcggcggc caaatgaagg acagcacaac gccagtaaag atggcagcca
     7681 ggccagaaat gatcggcaca aagcgtttac cggcaaagaa gccaagatac tcaggcagct
     7741 taatacggta gaaacggtta aacatgtacg ctgcgatcgc accggagata atccctccga
     7801 gtacgccagt atccgccagg tgtttagagg cgatttcttc agcaggtaaa tgcagtacca
     7861 gtggcgcaac cacggccatg gttttaacca tgatgccata ggcaacaact gcggccagcg
     7921 cggatacgcc atcgttattg gtaaagccga gggcgacacc gatcgcaaaa atcagtggca
     7981 tgtttgcaaa gacggaaccg cctgcttctg ccataacatg cgatacaacg gcgggcagcc
     8041 agctgaaatt cgcggaaccg acgcccagca gaatacctgc gataggcagt acggataccg
     8101 gcagcatcag cgatttaccg accttttgca ggttagcaaa tgcattctta aacataattg
     8161 agagtgctcc tgagtatggg tgcttttttt acgttctcac gcgtggcaag gggggagagc
     8221 ctcgccgtgt acagggcatc taagcgccct ttatttatta cacagagtaa aataattcag
     8281 tgccaatttg tttgacggct atcacgtttc aacttcagat cattggagaa gttgcacatt
     8341 ttgacaaaac atatgtcaca atatttcaaa cgtggttatt caccgcccaa ttttcggcgg
     8401 tgaactttac tcgttttagc cattaattac gagctttaaa aaaactcatt caacggatgg
     8461 attgaaggcg ggaagcgtcg atgtgaaaca gacgggcgaa gttatcggtg gttacctgcg
     8521 ccagttcttc aacggcaaca cctttcaaca cagccatgta ttctgcaacg tcacgaacca
     8581 tcgcaggttg attctctttt cctcgatgcg gtaccggcgc aaggtaaggt gagtcagttt
     8641 ccaccagtaa tcgatccagg gggacataac gcgcagcatc gcgcagttgc tccgcattac
     8701 ggaaggtcac aatgccggaa aaggagatgt aaaatccgag atccagtaat ttacccgccg
     8761 tttctctgtc ctctgtaaaa cagtgtagta cgccaccgca atccgtcact ttttcttcgc
     8821 gcagaattgc cagcgtatcg gcacgggcgt cacgggtatg gacgataacc ggcttgttca
     8881 gttcacgacc aatctggata tgatggatga aggactcttg ctgacgtact ttagtttccg
     8941 gtgtgtaata ataatccagc ccggtttcac ccagcgctac aacaccctct tctgccgcca
     9001 gacggcgtaa atcttctaca tcgtagggat cattctggtt gagtggatgc acgccacaag
     9061 aaaatacgac gttgtcacgt tcgcctacca gatcccgcat atgtaaataa cccggtaatg
     9121 ttgtggcgac tgccagacaa aatttcacat cgcgtgcggc ggctttcgcc agaacgtcat
     9181 ccacgtcctt atgcaaagat tcataatcca gaccatcgag atggcagtgt gagtcgacta
     9241 aaaacatgat gtctctctta aagatgagga accggtagca caacgcccgg ttgcaggtaa
     9301 tgctcaatac gcagtaaaag atcggtgatg agaagctcgc ggttgatgcc tgtaacagac
     9361 attaactgtt cacgaatgtg gcaaacatcc cccagtatag cctgcaggcg cgagggagaa
     9421 agatggtttg ccagttcggc gaccaggccc ggcacatcaa cattggtcac ctgcgcagca
     9481 ccatgatggc gttttagcgc atccatcagc aacgttgcca gccagtgtaa acgcgccgga
     9541 gcttgttcat gattaagggc cgctagcagc gaataccagt cgcccgatgg cacgctatat
     9601 gccaacgcct gacacaatgt ttcacgagcc tgccagttat ctccctgaaa caacgccagt
     9661 gccgcgccag gcgaaccggc gcttaagcgc aatgcggcaa gtaatgcatc ctgtgacatt
     9721 gtcacttcgc gtgaaagcca ggtcacggcg tactgttccg gcggcggcgc aaggtaatgt
     9781 aaccgacaac gactacgtaa tgttgccagt aaacgttcag gctcgcgggt agccaggaaa
     9841 aaccaggttt ctgctggtgg ctcttcaagc gttttcagca atgcgttagc cgcggcgtcg
     9901 gttagtaagg cagcatcggt tacccagaca actttcgcac cacctaagcg tgcgtgctca
     9961 ttcagctttt cggtgacctc acgtaccgca tcaatgccca acgcattttt ccctttttcg
    10021 ggagccaggg tgtagtaatc gggatgcgtg ccagcctgca tcaactgaca tccacgacag
    10081 tgaccgcaac ttttgtgacc ctgcggttgt tggcagagta aataacggct cagggcgtag
    10141 attaaagcat catcgcccat gcccggtaac gcctgaatga gcagcgcatg gtgacctctt
    10201 ccggcctgat agctggctac cagtttttcg aaatcaggtc gtaaccatgg ataccatctc
    10261 atgcgtccaa ctccttcacc cagtgggtca cggtagtgcg gattgcatcc atcacggcct
    10321 ccagcggctg ggtggcatca atggtatgaa tgcttttatc ttgtgctgcc agttccagat
    10381 agcgggcgcg ggtgcgatta aagaaatcga aagactcttg ctcaatacga tccagctcgc
    10441 cgcgcgcacg cgcgcgtttt aagccaactt ccggggtaac atcgagatag agcgttaagt
    10501 cggggcgaaa atccccgaga acagcatcac gcagtgttgc cagcatatgt tggtcaatac
    10561 cacgtcctcc gccctgatac gcctgagtgg agagatcgtg gcgatcgcca atcacccagg
    10621 tgccgttagc cagcgcaggt ttgatgactg tttctaccag ttgaacgcgc gcggcataaa
    10681 acatcagaac ttcggcttta tcggtaatga cttcatcgcc taccgatttg atatccagca
    10741 ccaggcttct taacttttcg gcaagttgcg taccgccagg ttcccgagtg aaaaccatgt
    10801 cgcggatacc cagttgctcg agcgtctcaa ccaccacatt acgcgcggta gttttgcctg
    10861 cgccttccag cccctcaatg acgatatact tactgcgcat ttttttcctt aagcactttc
    10921 agataatcct gcacagactt gttatgactg gcaagattgg tattaaacgt gtgaccacct
    10981 ttaccatcgg ccacaaaata gagatacggc gtttttgccg gatgcgcagc agccttcagc
    11041 gaatccgccc ccggcgtagc tatcgcaccc ggcggcaaac cggtaatggt ataggtgtta
    11101 tacgctgtcg gcgtttccag gtctgcacga gaaagtttgc cattataacg ctctcccatc
    11161 ccgtaaatca cggttgggtc ggtctgcagg cgcataccaa tgcgtaaacg gttgataaat
    11221 accgaggcaa cctgatcgcg ttcactggca acggcggttt ctttttcgat aattgatgcc
    11281 atcgtcacca gctgattttt atctttataa ggcagaccgt ccgcacgccc ttcccaggcg
    11341 ctatcgaccg ctttcaccat tttcttgtgc gctcgcttga gtaacgcgac atcggtggta
    11401 ttggcggtat acatccaggt gtctggccag aaccaacctt caatccactc cgggttttcc
    11461 agttcaagtg cctgcgctac ggtggcgtac ttatcgtcac tcagcgtgtg cttgatatac
    11521 ggagcctcac gcaattgctt gaggtagtcg ctcagacgca tcccttctac cagtcgcagc
    11581 gggaactgtg cttctttacc gctttccaac aatttcagca tctcgcgcac ggtcatctgc
    11641 ggtgtaaagc ggtaagtccc ggctttaaag tgagaaagat ccggttcgat acgcagcagc
    11701 cattgaaaaa cccgtgggcg attgatgatc ttatcagcat aaagctgttc accgagcgcc
    11761 agacgtccgg tccctggctt cagggtaaat atcgtctctt ctttgataag caatttgctg
    11821 tcggcaagat ggcgaacctt ccagacgccc acaccagcgg cgatacccag taccaccagc
    11881 aataacaaga taatcaataa cacttttttc atgactaatt cgggcgctca caaagtgggg
    11941 ctaaatattc atataacgtt gctgacgaaa aggagacatc gccacaggca cgtacgggca
    12001 tcactggcat taacgcatta caaataacca tctcatctgc ctgcaacgcc tcttccagag
    12061 aggcttgcac ttcgacaagc tgataagagg attgtgccag caaacggata cagaattgtc
    12121 gcataatgcc gttaacacct gcctgatcca gtcgcggcgt ataaactaca ttgcccttcc
    12181 gccagaacaa attagccgca cagcattccg taacccaccc ttcgctgtca aggaccagcg
    12241 cctcatcagc gtttgtctgc tcaagatgag agcggatcaa tacttgctca agccgattaa
    12301 gatgtttaat acctgcaaga tgaggattgc gccccagccg caccgggctt agcgccaacg
    12361 tcatcccttc gttacgcaaa cggtcgtaat gtgcaggata aaccgtaacg gagagaatcc
    12421 gcgttgccgg tccactgttc aatgtgctgt accctcgccc gccactaccg cgactgatca
    12481 cgactttcag cacaccattt tgctgttctg ctgccagcgt tttcatctct tgttcaagct
    12541 gaggccagaa gtcacagaaa atcatcaacc gctgacaagc atcctgtagt cgctggatat
    12601 gcgccgataa caaactgact ttaccgtcga taactctggc ggtggtaaaa caaccatcac
    12661 caaactgcgt tgcccgatcg cttactgcca gcgattcctg cttataaccg ttaattaaga
    12721 acataccggc tccttatcag agaattgtta gtgtggcagc atgttcacta cggaacaagt
    12781 cggaataaaa gctaagaaaa aaggcccgca agcggacctt ttatatgggt ggaaaatgac
    12841 aacttagatc tttttaaaga tcaaagaacc attagtgcca ccgaagccga aggagttaca
    12901 cagcgtgtat tccattccgc tgacctgacg tgcttcatgc ggtacgaaat ccagatcgca
    12961 accttcatcc gggttatcca ggttgatggt tggcggaaca gcctgatcgc gcagcgccag
    13021 gatggagtag atagattcta ctgcacccgc cgcacctaac aggtgaccgg tcatagattt
    13081 cgtggagctt accaacacac ggcttgcagc ttcaccgaag atggttttca ccgcctgcgc
    13141 ttcagcttta tcgccagccg gcgtagaagt accgtgcgcg ttaacgtagc caatctgact
    13201 cgcttcaatg cctgcatcac gcagagcatt tgccattgcc agagctgcgc ctgcgccatt
    13261 ttctggcggt gacgtcatat gataagcatc gctgctcata ccaaagccga cgagttcagc
    13321 gtaaattttc gcaccgcgtt ttttcgcgtg ttcgtactct tcaagtacca gcataccggc
    13381 accatcgccc agtacgaagc catcacgctc tttatcccac gggcggctcg ccgcttgcgg
    13441 gttatcattg cgggtagata atgcacgtgc cgcgccaaaa ccaccaacgc ccagcggcgt
    13501 actggctttc tctgcgccac ctgcaaccat cacgtcagca tcgccatacg cgataatacg
    13561 cgcagcatga ccaatgttgt gcacgccgga agtacaggcg gtcgcgatag agatgcttgg
    13621 gccacgcagg ccatacatga tagtcagatg acctgccacc atgttcacaa tcgttgacgg
    13681 aacgaagaat gggctgatct tacgtggacc accgttcatc agagatgtgt ggttttcttc
    13741 gatcagtccg aggccgccaa tcccggagcc aattgcggca ccaatgcggg ttgcgttctc
    13801 ttccgttatt tcaaggccag aatcctgcat ggcctgaacg ccagcgacaa ttccatattg
    13861 aatgaaggca tccatcttgc gctgttcttt gcgcgagata atgtcctcac agttaaaatc
    13921 ctttactaag ccagcaaatt tcgttgcata ggcgctagta tcgaaatggt cgattaggct
    13981 gatgccactc tgaccggcaa gcagagcttt ccaggtagac tctacggtat tgccgacagg
    14041 agacaacatg cccagtccgg tcacaactac acgacgctta gacacgtttg tcctccaggg
    14101 agggaaaaaa tgattctagt gggacaaaaa gataaaactc aggcggtcga acgaccgcct
    14161 ggagatgttc acttacgcct ggtggccgtt gatgtaatca atggcagcct gaacggtggt
    14221 gattttctca gcttcttcgt ccggaatctc agtatcaaac tcttcttcca gagccattac
    14281 cagctcaacg gtgtcaagag aatccgcgcc caggtcttca acgaaagaag cattgttggt
    14341 aacttcttcc tgcttaacgc ccagctgttc gccgataatt ttcttaacgc gttcttcgat
    14401 agtgctcata ctcttaaatt tcctatcaaa actcgctttc gcgatggttt tcgtagtgta
    14461 taaaatgttg aaaaatttgc aactaaatcc cggcaggtct taccacgatt ttacgttatt
    14521 ttgaggcctt accccccaat aacgcaaatc attttgcact aattattact cataaccacg
    14581 cagactgcgc gcaacatgag cattttgtgc aaatcgcggt cagaccatgt acatcccgcc
    14641 gttcacatgc aaagtttcac ccgtgatgta agctgcttcg tcggatgcca ggaatgcaac
    14701 cgcgttggcg atttcctgtg cgccgccgag gcgacccgca ggaacctgcg ccaggatacc
    14761 cgcacgctgg tcatcgctca gcgcacgtgt catgtccgtt tcaataaagc ccggagcaac
    14821 aacgtttaca gtaataccgc gtgacgcaac ttcgcgcgcc agtgatttac tgaagccgat
    14881 caagcccgct ttcgccgcag cgtagttggc ctgaccgcca tttcccatgg taccaaccac
    14941 agaaccgata gtgataatac gaccatgacg ctttttcatc atagcgcgca ttaccgcttt
    15001 tgacagacgg aaaacagatg aaaggttggt ttcgataata tcgttccact cttcatcttt
    15061 cattcgcatt aacaggttat cacgagtgat accggcatta ttgaccagga tatccacttc
    15121 accaaattct gcgcgaattt tttccagaac agattcgata gatgccgggt cggtcacatt
    15181 caacatcaga cctttgccgt tagcacctaa ataatcactg atcgcctgag cgccattttc
    15241 actggtcgca gtgccaataa ctttcgcgcc acgggctgcg agcgtttcag caattgcgcg
    15301 gccaattccg cggcttgcac cggttaccag tgcgattttt ccttcaaaat tcatgatttt
    15361 cctcttttaa agctcgagcg ccgctgccat cgctgaaggt tcgttcagcg ccgaggcggt
    15421 cagggtgtcg acaatgcgtt tcgtcaggcc agttagcact ttgcccgggc cgacttcata
    15481 gagatgttct acgccttgcg ctgccatgta ctcaacagac ttcgtccact gaaccgggtt
    15541 atacaactga cgtaccagtg cgtcacgaat ggcatcacca ttggtttcgc atttcacatc
    15601 aacgttattc acaacaggaa ctgttggtgc gttaaaggtg attttcgcta attctactgc
    15661 cagtttgtcg gctgctggtt tcatcagcgc acagtgagac ggtacgctca ctggtaacgg
    15721 cagcgcgcgt tttgcgcccg cagctttaca ggcagcgcca gcacgctcaa ccgcctcttt
    15781 atgaccggca ataaccacct gtcccggaga gttaaagttt accggagaaa cgacctgacc
    15841 ttctgcagct tcttcacacg ctttcgcaat agacgcatca tccagaccga tgattgccgc
    15901 catagcgccc gtgccttccg gtacggcttc ttgcatgaac ttgccgcgca tctcaaccag
    15961 acgcaccgca tcagcgaaat caatcacacc agcgcaaacc agcgcggagt attcccccag
    16021 gctgtgaccg gccatcattg ccggtgcttt accgccctgc tgctgccata cgcggtacag
    16081 cgcaacagat gcagtcaaca gcgctggctg ggtttgccag gttttattca gttcttcagc
    16141 tggcccctgc tgggtcagcg cccacaggtc gtagcccagc gccgcagaag cttcagcaaa
    16201 cgtttcttcg acaattggat agctcgccgc catatcagcc agcattccaa cggtttgaga
    16261 accctgtcca gggaacacaa atgcaaattg cgtcatgttt taatccttat cctagaaacg
    16321 aaccagcgcg gagccccagg tgaatccacc gccaaaggct tcaagcagaa ccaactgccc
    16381 cggcttaatg cgcccgtcgc gtacagcttc atccagcgcg cacgggacag aggccgcaga
    16441 ggtattaccg tggcgatcca gcgtcaccac gacattgtcc atagacatac cgagtttttt
    16501 cgccgttgca ctgataatac gcaggttagc ctgatgcgga accagccagt ccagttgaga
    16561 acggtcaaga ttattcgccg ccagcgtctc atcaacgatg tgcgccagtt ccgttaccgc
    16621 aaccttgaag acttcgttgc ccgccatcgt cagatgaatt gaattctctg gattcacgcg
    16681 gtcggcgttt ggcagcgtca gcaattcacc ataactaccg tcggcatgca gatgggtgga
    16741 gatgattccc ggctcttcag aggcagccag caccgcagcg cccgcgccat cgccaaaaat
    16801 aataatagtc ccacgatctg ttggatcgca ggtgcgcgcc agtacatcgg aaccgacgac
    16861 cagagcatac ttcaccgccc cagatttcac gtattgatcg gctacgctta atgcataggt
    16921 gaaacctgcg caggctgctg caacgtcaaa tgccgggcaa cctttaatgc ccaacatgct
    16981 ttgaatctga caagctgcgc tcgggaaagc gtgcgtagca gaagtcgttg ccacaacgat
    17041 caggccaatc tggtctttct caatgcccgc catctcaatt gcgcgtgtcg ccgcttcaaa
    17101 gcccatggtt gaaacggttt cgtttggcgc ggcaatgtgg cgttcgcgga taccggtacg
    17161 agtgacaatc cactcgtcag aggtgtccac cattttttcc aaatcggcgt ttgtccgcac
    17221 ttgttcgggc agatagctgc cagtaccaat aatcttcgta tacatgtacg ctcagtcact
    17281 tttcggttat ataccgtcac ttgcaaactg cgagttcgct ggcagcgtcc tgctaccgca
    17341 gagttccgct tttgccaccg tccagcagct caaaaccagc tgggtataca gattccaggc
    17401 gagcggcaat tcgctgagga acttgtcgct gcaccgcctg cactgcctgt tcaatcgcga
    17461 ccgcaaaagc tcgctgattg gctgcaccat gactttttat caccgtgccg cgcaatccta
    17521 acagacaggc gccgttatac tggtcggggt tgaggtgact gaatcgcctc gtcaggctct
    17581 tttgtagcca acgctttaat aacagtagcc accacgaccg ttttttccct tcaccctgag
    17641 atttcagcag agaaaggaac atcctgacaa caccttccat cgtctttaac gtgacatttc
    17701 ctgtaaagcc gtcacaaacc agcacatctg tcttgccagt taacaactca ttggcttcaa
    17761 gatagccgat ataattgata gaagggattg ttttaagcac cgctgaggca tcccgaatac
    17821 tgtcgagacc ctttacttct tcttcaccaa tattgagcaa cgccacgcga ggattgggaa
    17881 tttccaccac ctcttcagcc agaactgagc ccataatggc aaattgcacc aacattgtgc
    17941 tgtcacaatc gacgttggcc cctaaatcga ggaccaccgt tttgcccttt tgctgatgtg
    18001 gtaataccgt caccagcgcc ggacgctcaa tcccctccag gggcttgagt aataattttg
    18061 ccagccccat cagtgccccg gtattaccgg cactgacaca ggcttgcgct cgaccttctt
    18121 tcaccagctc cagggccata cgcattgaac tcccacgact ggcgcggata gcttgcgaag
    18181 gccgggcatc actggcgata actgactgcg caggaataat ctgcagacgc gaacgttgtt
    18241 caaagtcagc tttagcaagt aatggcgtga tggcgtcggg attgccgact aaaagaagag
    18301 tgagttgcga attagaattc agtgcctgca atgctgcagg cactgtcacg gaagggccaa
    18361 aatcccctcc catgacatct aacgccaggg ttagacgtgt caaggtatcg tcgccgcccg
    18421 gtttggtctt tccccagttg cctggggaaa atcctcacta agcttcatca cgcagacgca
    18481 tgattactta gcgatgacct tgcggccgcg gtagtaaccg tcggcagtga tgtggtgacg
    18541 caggtgtttt tcaccagaag ttttgtctac agacaggctg gtgactgcgg tcagcgcgtc
    18601 atgggaacga cgcatgccac gtttggaacg ggttggttta ttctgttgta cggccatgga
    18661 ccttactcct caattacggt ttatccccga tccaacgggg agcaccaatt acttacgctt
    18721 taagctggct aatacggcaa atgggtttgg cttttgcgct tcttcaggca gttcaccaaa
    18781 gaccatgtcc gcttcggaca cttcacagtg ttcagaatca tgcaccggaa ctaccggcaa
    18841 ggcgaggatg atttcatctt caaccattgc aagcagatcg atttcaccga attcgttaac
    18901 ctcaatcggt tcatacgctt ccggcagtgc ttcagcctgt tcgtctgaac gcacaggact
    18961 aaaacaatac gttgtgtaga cctgatgagt aaacggcttc ccgcaacgct gacactcgag
    19021 cgttaccgtc accttcgcat cgccgtttaa cactgcgaga cgttggttat cgatagcgaa
    19081 cgacatggag cattccacat cactgtccac actgactacg gattcggcga cgcgctcaac
    19141 ctgatcaggg gtatagatac cctggtaatc aaggcgtttt tgagccgtac gaaccggatc
    19201 gagagtcagg ggtaatttta ccttttgcat agggcgcgca tattaacttt gtaacgtcat
    19261 agagtcaaag aaaaaggcag cctgtggttg ccttttgcca ataattcgca cacattgcgg
    19321 gttactgctt tattttcgtt cagtgctgat aacgaacatc aacctgcaaa acgaagcgca
    19381 tagtttaaaa tggctatcat caatacgcta tatctgatgg aaaaaaatat gcctaaactt
    19441 attttagcct ccacctcgcc atggcgtcgc gctcttctgg aaaaactgca aatctctttt
    19501 gaatgtgcag caccagaggt cgacgaaacc ccacgcagcg acgaatcacc gcgacagttg
    19561 gtgcttcgac tggcacaaga aaaagcgcaa tctctggcgt cacgttatcc ggatcattta
    19621 attattggtt cagaccaggt atgtgttctt gatggcgaaa tcaccggtaa accgttaacg
    19681 gaagaaaatg cccgtctgca attacgcaaa gccagcggca atatcgtcac cttctatact
    19741 ggactggcgt tgtttaattc ggcgaatggg catctacaaa cagaagtgga gccttttgac
    19801 gtccatttcc gtcacctgag cgaggcggag attgataatt acgtgcgtaa ggagcatccc
    19861 ctgcactgcg cgggtagctt taagagtgaa ggatttggca ttacgctgtt tgagcgttta
    19921 gagggacgtg accctaacac gctggttggt ttaccgctaa tcgcgctgtg tcagatgtta
    19981 cgtcgggaag ggaaaaaccc gctgatggga tagttgtttg cgccggatgc ggttacgtcg
    20041 catccggcaa tcggtgcagg cggcctgatg cggcgtaaac gccttatcag gccaaccgcc
    20101 cattatcagg cttatatgct tgtattagcg cgcgttacgc agcttttgca aacaacgctt
    20161 taaaccttca tccatcggcg cttcgatacg catcacttca ccggtccccg gatgggtaaa
    20221 cttcaacgct gcggcgtgca ggaacagacg atttaatccc gtgcctgctt cagtgagctg
    20281 tctgtcaaat tcacggtcac cgtagcgatc gtcaaaggca atcggatgac ccgcatactg
    20341 tgtatgcaca cggatctgat gagtacgtcc ggttaccggg ctacaacgca ccagggtggc
    20401 aaatgcatag cgttcttcca ctttaaagcg tgtttccgac ggtttgcctt cctgactgac
    20461 acgcacgata cgttcgccgc tttgcagaat atttttcaat aacggcgctt gaacgctctt
    20521 cacatgcgac tgccactgac cgcgcaccag cgccaggtaa tctttttgca ttcctttttc
    20581 acgtaattgc tcatgcagag aacgcaacgc cgagcgtttt ttcgctacca gcaacacacc
    20641 tgaagtgtcc cggtcgagac gatgaaccag ttcaaggaac cgcgcttccg ggcgcaacgc
    20701 ccgcaaacct tcgataacgc cgaagcttaa accactgccg ccatgtaccg ccgtaccgga
    20761 aggtttattc agcaccagga tatgatcatc ttcatataag atgacgtccg ccagcgccgc
    20821 caccttttgc agatgtggcg aaaccgcctc ttcttcccgc tcagcaacgc gaaccggtgg
    20881 gatacgcacc tcatcacccg cttcgagttt atattcaggc ttaatacgtt ttttgttcac
    20941 ccgcacttca cctttacgca aaatacggta aatcatactt tttggtacgc ctttcaattg
    21001 ggtacgcaaa aagttatcga tacgttgccc cgcttcgtca gcggtgatag caacaatttt
    21061 tacggatgga gtctctgttt tcatggtggg cgattctaat tagccaacag gattcgcgcc
    21121 actcattttt ctatgcttat atttagtttt acaccttatt acttcactac gtgatcactt
    21181 tattgatggt tattaaacca atcaccagca agaagtgaaa aaactgtgag taagcgggtg
    21241 ataaatggta aaagtcatct tgctataaca aggcttgcag tggaataatg aggccgtttc
    21301 cgtgtccatc cttgttaaaa caagaaattt tacggaataa cccattttgc ccgaccgatc
    21361 atccacgcag caatggcgta agacgtattg atctttcagg cagttagcgg gctgcgggtt
    21421 gcagtcctta ccggtagatg gaaatatttc tggagagtaa tacccagtct gtttctttga
    21481 taattgcgct gtttttccgc atgaaaaacg ggcaaccgac actctgcgcc tctttgagct
    21541 gacgataacc gtgaggttgg cgacgcgact agacacgagg ccatcggttc acacccggaa
    21601 aggcgttact ttgcccgcag cttagtcgtc aatgtaagaa taatgagtaa gttacgatga
    21661 aaagaatgtt aatcaacgca actcagcagg aagagttgcg cgttgccctt gtagatgggc
    21721 agcgtctgta tgacctggat atcgaaagtc cagggcacga gcagaaaaag gcaaacatct
    21781 acaaaggtaa aatcacccgc attgaaccga gtctggaagc tgcttttgtt gattacggcg
    21841 ctgaacgtca cggtttcctc ccactaaaag aaattgcccg cgaatatttc cctgctaact
    21901 acagtgctca tggtcgtccc aacattaaag atgtgttgcg tgaaggtcag gaagtcattg
    21961 ttcagatcga taaagaagag cgcggcaaca aaggcgcggc attaaccacc tttatcagtc
    22021 tggcgggtag ctatctggtt ctgatgccga acaacccgcg cgcgggtggc atttctcgcc
    22081 gtatcgaagg cgacgaccgt accgaattaa aagaagcact ggcgagcctt gaactgccgg
    22141 aaggcatggg gcttatcgtg cgcaccgctg gcgtcggcaa atctgctgag gcgctgcaat
    22201 gggatttaag cttccgtctg aaacactggg aagccatcaa aaaagccgct gaaagccgcc
    22261 cggctccgtt cctgattcat caggagagca acgtaatcgt tcgcgcattc cgcgattact
    22321 tacgtcagga catcggcgaa atccttatcg ataacccgaa agtgctcgaa ctggcacgtc
    22381 agcatatcgc tgcattaggt cgcccggatt tcagcagcaa aatcaaactg tacaccggcg
    22441 agatcccgct gttcagccac taccagatcg agtcgcagat cgagtccgcc ttccagcgtg
    22501 aagtccgtct gccatccggt ggttccattg ttatcgacag caccgaagcg ttaacggcca
    22561 tcgacatcaa ctccgcacgc gcgacccgcg gcggcgatat cgaagaaacc gcgtttaaca
    22621 ccaacctcga agctgccgat gagattgctc gtcagctgcg cctgcgtgac ctcggcggcc
    22681 tgattgttat cgacttcatc gacatgacgc ctgtacgcca ccagcgtgcg gtagaaaacc
    22741 gtctgcgtga agcggtgcgt caggaccgtg cgcgtattca aatcagccat atttctcgct
    22801 ttggcctgct ggaaatgtcc cgtcagcgcc tgagcccatc actgggtgaa tccagtcatc
    22861 acgtttgtcc gcgttgttct ggtactggca ccgtgcgtga caacgaatcg ctgtcgctct
    22921 ctattctgcg tctgatcgaa gaagaagcgc tgaaagagaa cacccaggaa gttcacgcca
    22981 ttgttcctgt gccaatcgct tcttacctgc tgaatgaaaa acgttctgcg gtaaatgcca
    23041 ttgaaactcg tcaggacggt gtgcgctgtg taattgtgcc aaacgatcag atggaaaccc
    23101 cgcactacca cgtgctgcgc gtgcgtaaag gggaagaaac cccaacctta agctacatgc
    23161 tgccgaagct gcatgaagaa gcgatggcgc tgccgtctga agaagagttc gctgaacgta
    23221 agcgtccgga acaacctgcg ctggcaacct ttgccatgcc ggatgtgccg ccagcgccaa
    23281 cgccagctga acctgccgcg cctgttgtag ccccagcacc gaaagctgca ccggcaacac
    23341 cagcaactcc tgcacaacct gggctgttga gccgcttctt cggcgcactg aaagcgctgt
    23401 tcagcggtag tgaagaaacc aaaccgaccg agcaaccagc accgaaagca gaagcgaaac
    23461 cggaacgtca acaggatcgt cgcaagcctc gtcagaacaa ccgccgtgac cgtaatgagc
    23521 gccgcgacac ccgtagtgaa cgtactgaag gcagcgataa tcgcgaagaa aaccgtcgta
    23581 atcgtcgcca ggcacagcag cagactgccg agacgcgtga gagccgtcag caagctgagg
    23641 taacggaaaa agcacgtacc accgacgagc agcaagcgcc gcgtcgtgaa cgtagccgcc
    23701 gccgtaacga tgataaacgt caggcgcaac aagaagcgaa ggcgctgaat gttgaagagc
    23761 aatctgttca ggaaaccgaa caggaagaac gtgtacgtcc ggttcagccg cgtcgtaaac
    23821 agcgtcagct caatcagaaa gtgcgttacg agcaaagcgt agccgaagaa gcggtagtcg
    23881 caccggtggt tgaagaaact gccgctgccg aaccaattgt tcaggaagct ccagctccac
    23941 gcacagaact ggtgaaagtc ccgctgccag tcgtagcgca aactgcacca gaacagcaag
    24001 aagagaacaa tgctgataac cgtgacaacg gtggcatgcc gcgtcgttct cgccgctcgc
    24061 ctcgtcacct gcgcgtgagt ggtcagcgtc gtcgtcgcta tcgtgacgag cgttatccaa
    24121 cccagtcgcc aatgccgttg accgtagcgt gcgcgtctcc ggaactggcc tctggcaaag
    24181 tctggatccg ctatccaatt gtacgtccgc aagatgtaca agttgaagag cagcgcgaac
    24241 aggaagaagt acaagtgcag ccgatggtga ctgaggtccc tgtcgccgcc gctgtcgaac
    24301 ctgttgttag cgcgccagtt gttgaagaaa tggctgaagt cgtagaagcc cccgtaccgg
    24361 ttgccgaacc gcaaccggaa gtggttgaaa cgacgcatcc tgaagtgatt gccgccgcgg
    24421 taactgaaca gccgcaggtg attaccgagt ctgatgttgc cgtagcccag gaagtcgcag
    24481 aacacgcaga accggtggtt gaaccgcagg aagagacggc agatattgaa gaagttgccg
    24541 aaactgctga ggttgtggtt gctgaacctg aagttgttgc tcaacctgcc gcgccagtcg
    24601 tcgctgaagt cgcagcagaa gttgaaacgg tagccgcggt tgaaccagag atcaccgttg
    24661 agcataacca cgctaccgcg ccaatgacgc gcgctccggc accggaatat gttccggagg
    24721 caccgcgtca cagtgactgg cagcgcccta cttttgcctt cgaaggtaaa ggtgccgcag
    24781 gtggtcatac ggcaacgcat catgcctctg ccgctcctgc gcgtccgcaa cctgttgagt
    24841 aataattagc tcaaagtaat caagccctgg taactgccag ggctttttta tttcatgttt
    24901 gaatcaacat attaaaattc atcaaatggc atccttgcta accaacaatg caaaataggc
    24961 aaaaaaaatg cccgccccgg cgatcccaga gcgggcagac aaaacatacc cagtttcatg
    25021 atatgtttta aagcgagctt atttgttgag ctggaagagc gacaatccct gcatatcggt
    25081 aaatgcttta tacgatgcct gcaatgccgt ttgctgcatg atgtaagatg aaatagttgc
    25141 attccagtcc acatcaacca gatcgctcat ctgctgcgtt tgccctaaag cgcgatcgct
    25201 acctaaggaa tccagcgact ccagttcgtt cagctgcgtg cctaattccg cgcgaacggt
    25261 aaggacatta ttcagcgagt ttttcagtcc acggttggtt ttatccaatg ccgcagcggc
    25321 gatttcttta tccgcttcgc tatccgcgac cggcgttttc agggctgcga tggcgctatc
    25381 cagcatggca aaaagattgg tttcagaagc gctaccgtct ggttccgcta ccgcgttact
    25441 ggtaatactg tcgaaaattt tgtcgcccgt atgtcctatc accatcgaac gcgaagcatc
    25501 gacctgttgc ctaatacttt ctgctccacc gacgtatttc cctttctctt cgctaaacgg
    25561 cgcagtctct gttttataac cggcaaaaat gtagcgcccg ttaccgtcag tggtgttcgc
    25621 cagattcagc aactggtcac gaagcccctg gatatccgta gccagcgagg cccggtcatc
    25681 gtcactcaaa gtgccattgc tggcgtagac aattttttcc tgagcattct ggatagcagt
    25741 ggtgacctgg ctaagtacac tctcttccag tgacactttt tgagtggcga aagtacgcgc
    25801 cagcgtgtac tggctgtttt gcgcctgtgc ctgggagaga acaaccgctt gtgatgcagc
    25861 aatgggatca tctgaagggt taacgactcg cttacccgtc gacatctgtt cgccgtactt
    25921 catccattct gcctgagaat tggtgatgcc acgcatgttt tgctggtaca tcatctgggt
    25981 actgaaacgc atcttatctc cccttagcga atgttaatca gcgcatcaaa aatcgcgttt
    26041 gccgtctgca gaacctgcgc attcgccagg taatactgct gaaaacgttg cagatttccg
    26101 tactcctcat cgagattgac accggaaatc gactgctgct gattggaaag ctgcgtcacc
    26161 acattacctt gcgtggtgct actggttttc aacgtcgcgg ttttattacc gatatcactc
    26221 actaacgagg cataagcgtc gttaaaggat ttcgcaccgc ccaccgtttt actgttgctt
    26281 tgcagatcca gcagggcctg accgttgcgg ttgtcgctat cacccgcatc ttcttcgctc
    26341 gccatcgcga ttttcgcttc gtcggtgatt aatacatcca tgttgacgat ggcgtcactt
    26401 actggtttca gcgtgaagct gtcgttaacg gcaggcgttc ctgtaaacgt caactccaga
    26461 ccatcaaatg ccactttacc gttggcatcc ggcgtcaccg taaaagtggt attgctggca
    26521 aggcgggtga cctgccactg attattatcg aacgagattt tgtaatctgt cgccagtacc
    26581 ccggaggcat cagttaccgt ggcaccgatc gcaacgtcac ctttgttttt cgtgttttgc
    26641 agaatcgcgg gcttaccgat agcaaagaaa tcttcaccag catcgccgtt ggcatcaaat
    26701 ccggctttgt gttgggtgtt gaaagcctcg gcaaatgcca gcgccagttg tccaagcgta
    26761 ttacgcgtct ggtccagatc ctgagaacgg aatgtcagaa tgccgcccag cgacccggta
    26821 ttcagcaatt tctccgggat ctcaatattg cctgccgtcc catcaacgta agcgacagtc
    26881 gtacgagaag ggtcagcgct ggaaggaact gccgccagtt gccgagccgt acttccctga
    26941 accagtgagt aaccattggc catcgtgatg ttataagtgc cgccatcctg aacgctgact
    27001 tctacaccaa caatctggtt taattcgctc accagttgat cgcgttgatc cagcagattg
    27061 ttaggtgacg cccctgcccc cacgcctgtc aggcgcgaga tttgatcgtt caggctggca
    27121 atttgtttag cgtaattgtt gatctgatca acgctggcac ctatcgcgat attgacctgt
    27181 ttgtcctggt cgcgaagata ctgatcggtg gttttaaact gattcaccaa cccttctgat
    27241 ttcccaatca gcgcctggcg cgctgccggg tcttccgcgt tactcaccag cgtttgcagg
    27301 ctggtgaaga aatcctgcat ctgtgttgcc agcgaagagg tactggtgga gagcatattg
    27361 tcgattttcg acatctgctc atagcgggca gtcaggccgc tactttgcgt ctgcgccgca
    27421 cgtaactggt tggtaataaa cgcatcatac tcacgctgca caccagaaac gttgacgcca
    27481 ttgccaaccc agccgccagc gcccaacgtg ctattggcct gcgccataat agtggtttgg
    27541 cgggtatatc cagcaacgtt atagctggag atattattac ttgccgtatt taacgccgcc
    27601 tgggccgcgt tcagtccgct catggcgtta ttgatcaagc tggacatgga ggttccttat
    27661 aagccttcaa ttacagagta ttatcggcag cgacccgccg gacttgagtt attcagaaca
    27721 gattatcaat gttcatactg taggttttgc tcaccttgtc gcttatcgat ttcatctgct
    27781 ggatcatgtt ggtgagttta cgggcatagt gaggatcggt ggcatagccc gcgtcctgta
    27841 gggcctgcgc cccctgttcc gcactcgcgg cggtcgtcac ggcagcgtag cgcgggttac
    27901 gtgttaacaa cccaacgtaa tccgacaatg cttccagata cgagctgtag acccgaaact
    27961 tcgcttttac tttcttcgct tcgccgtttt catattcagt cgtggtgatt tcagtaactg
    28021 gccctttcca gttgccagag gctttgacac caaacaggtt atagcttggc tcgccgtttt
    28081 cgcggcggat ttgccgttgc ccccaaccag attcaagcgc cgcctgagcg aggatcaaat
    28141 gatgtggcac accgctttgc tggcttgcca gttgggcggg cagcgagagt tgcgcgagga
    28201 atgctttact gtcacccggc agcgaatcat cgtagttacg tggcacggcc ttttgcacca
    28261 gctgcgaaag cgcctgattt tgataacgca ccacggtttc gagcgggaat ttcatcggtg
    28321 ctgctggcgt gggctcctct ggcaatggtt gttctggcgt catctgttta accatcatct
    28381 ctgcaagccc cagacctttg cccgccgtca tctgttgggc aatctgctgg tcatacatac
    28441 tggtatacag gcgagtgtgc tcgctgctga acaggccatc ttttggtaaa gcgtcgcgca
    28501 tgcttttcaa catcatctgc acgaacatcc cttccacctg acgggccacc ggacggatat
    28561 ttgccgccgg gtcttcgccc gctttcgcct ttagttcgtt gagtgattgc gcatcccagg
    28621 ccgcacttgc cagtagtttg ctgtcgctga tcatcagatg atttccagtt ttgcccgcag
    28681 acatcccgca ctttgcattg attgcaaaat agacatcaga tccatcggcg tagcgcccag
    28741 cgcattgagc gcgcgcacca cgttattgag gctggcgctg gaacgtacgc tttgcagcga
    28801 accgccgctc tggcgtaaat cgatctgcgt ttgtggagta accacagtct gtccaccacc
    28861 aaacggtgta tctggctggc tgacattggc ctgacgattg actgttactg agagatttcc
    28921 ctgcgctacc gcgcagctgt cgagggtcac ttcgcgattc atcaccaccg aaccggtgcg
    28981 cgagttaatc actactttag cgtcctgcgg ggtgacatta acctgcatat tctggatatc
    29041 tgcaaggaaa cggacctggg aactgttgcc acttggtacg cgcacctgaa tagtccgcgc
    29101 atctaacgcg gtggcgctgc catatccacg cacgcggttg atggtgtcag cgatttgctg
    29161 cgccatgctg aaatcttcgt cgttaagttg caaattaagg gtattcccga cgccaaactg
    29221 gctgggcaat tcacgttcaa taaccgcacc attggtgatc cgtccaccgt tcagttggtt
    29281 cacctgaaca ctgctaccgc cagcggaggc tcctgcgccg ccaaccagaa tattgccctg
    29341 cgccagcgca tacacctgac tgtcaacgcc cttaagcggc gtcatcaaca acgtacctcc
    29401 acgcaagctt ttggcatttc ccatggaaga aaccaccaca tcgatggttt gcccctgacg
    29461 tccaaacgga ggaagtgacg ctgtcaccat taccgcagcg acgtttttta gctgcatatt
    29521 ggtgcccgtc ggaacggtaa ttcccagctg tgagagcatg ttattaagcg tttgtgtggt
    29581 aaacggcgtc tgggttgtct ggtcaccggt gccatccagc cccaccacca gaccatagcc
    29641 aatcagtgag ttttgcctta ccccctgaac actggtgaga tcgcgaatac gctcagcctg
    29701 agccgccgtc gtaaccagta gaagaattaa tgcagagaga aatttaatca ccacagcctc
    29761 acttacattg gcgacaggtt aaggaagaaa cgctgcaacc agcccatatt ttgcgcttcg
    29821 ttaatgtagc cattgcctac gtattcaatg cgcgcatccg ccacctgagt agacggtacg
    29881 gtattgctgc cgctgatagt gcgtggatta accacgccag agaagcgaat aaattcggta
    29941 ccctgattaa tggcaatctg tttttcaccc accacatgca ggttgccgtt gaccagtacc
    30001 tggtcaaccg tcaccgtcaa cgtgccgtta aaggtattgc tggcattagc cccgcccttt
    30061 ccgttgaacg tgttaccacc ggaggcttcg acatcggcac gagcgttacc aaacagcccc
    30121 tgcaaatagc gcggcacagt atcaaagcca aaattagttt taccgtcacg gctggcattc
    30181 gcataggagc ttttgctggc gctgacgttc tcctgcaaca cgatggtcag cgtatcgcca
    30241 atattgcgtg gtcgacgatc ttcaaacagc ggttgatagc catagttaat cggctgagca
    30301 gactggaaaa tagaaccgtt ggcgacgggc gtcggcccgg gaaccggttg tgcactggtc
    30361 gccccctgca ccagcggcgt ggagggtatc caggcgcagc cggttagtga aagcaccaac
    30421 aagctggaaa tggcataagt atgcgcagcg tttttttgca tggctatcat cttcaaaaat
    30481 cagtaaaccg gtgaacctgc caccggttaa gccttagagt tgcgtcagtt tttgcagcat
    30541 ctgatcggtg gtggacaccg ctttactgtt gatttcgtaa gcgcgttgca cctgaatcat
    30601 attgaccagt tcttccgcca cgttgacgtt agacgtttca acataccctt gatacagcag
    30661 tcccgcgccg ttcaggcccg gcgtgctttc gttcggtgca ccagaggatt gcgtttcggt
    30721 gtagaggttt tcgccaatgc tctccagccc ggtgtcattc ataaaggtgg tgagattgag
    30781 ctgcccaacc tgaaccggag ctgcctggcc ttgttgggtt acgctgacca cgccatcacg
    30841 accgatggtg atacttaacg cattcgccgg aatggtgatc gctggctgca cctgaaaacc
    30901 accagccgtc accagctgcc cgttctgatc cacctggaaa gagccgtcac gggtataggc
    30961 tgatgaacca tctggcaaca tcacctggaa aaagccctgc cctttaatcg cgacatcttt
    31021 gctgttgttg gtctgcgaca ggtttccctg gctgtgtaag cgttcagtgg cgaccgggcg
    31081 tacccccgtg ccgatttgta atccggaggg taaggtggtt tgttcggaag actgtgcccc
    31141 cggctggcga atggtttgat aaagcagatc ttcaaacacc gcgcgctgac gcttaaaacc
    31201 gttagtactg acgtttgcca ggttgttggc aatgacgtcc atattggttt gctgggcgtc
    31261 aaggcccgtt ttggcgatcc ataatgaact gatcataaaa tgtcctgtag tggattagtg
    31321 acaagcagag cagaagcacg cgacagtgtg aatttcccct caccctaacc ctctccccaa
    31381 aggggcgagg gaatagtccg cgcgctttta ttgcaatgtc atcaatcgtt caattaactc
    31441 atcgacagca gttggttggc acggcctgcg ttatcatcga cgctgctgat caccttcatc
    31501 tgcatttcaa aacgccgcgc gctggcaatc atgtcgctca ttgccgcaac ggcattgacg
    31561 ttactgcctt ccagaacccc cgacatcaca cgcaaggttg gatctgcctg cagtatcggc
    31621 ccacgcgtgg cctggctttc tgcgcttaaa cgaaaaatgc cgtcgtcacc gcgttgcact
    31681 tcgctgcccg tggctttcac cagtttcaga cgccccactg gcgcaaccgt atttgccgga
    31741 tcgcccggat tgagcgccga gattgtgcca tcggcagcaa tagtgatttc cgccccttcc
    31801 ggcacagcaa ttggcccagc ctcgcctatc accggatgcc cctgaattgt cagttgcccg
    31861 gtgggatcaa cctgaatgct gccattacgc gtataccctt cgctgccgtc agcggactgc
    31921 acggccagcc agccatcctg ctgcaatgca acgtccagcg ggcgcgaggt gtaatccatc
    31981 ttgccgggcg tcatgtctgc acccggtgtt gacgccgtta ccagcgtgcg cgtgggcagt
    32041 gaaagccctt ccaccggcac tgcacgcaag gcgtttaact gcgcgcgaaa accgggcgta
    32101 gaggcattgg ccagattact ggcggttacc gcctgttgat tcagcgtctg gctggctgct
    32161 cccatcgcgg tataaattgc gtgatccatt gagctatccc gtcagcgatt agcgtaagtt
    32221 aaccagcgtg ttgaggatct ggtcctgggt tttgatggtc tgggcgttag actgatagtt
    32281 acgctgggca acgatcatat tgaccagttc tttactgaga tcgacgttgg acgcttccaa
    32341 cgcaccgttg gtcagggtgc caaagttgcc cgtcccggct gtccccaaca gcgccacgcc
    32401 agaagattgc gtcgcagacc agacgttgtc gccttcggat gccagacctt cgttgttggc
    32461 aaagttcgcc agtacaatct gccccagcag ttgtgtttgt tcgttggaat agttgccgac
    32521 aaccgtaccg tcatcattga tttgataact caccagatcg cccggtttgt agccattctg
    32581 ggtggttgcc acaatattgt tagcgccggt attttgctgc atggagttga ggaagctcag
    32641 gctaaacgtg gcgggatctg cgccgttaat tgcgccagtg gtaatattct ctgttggatt
    32701 tgaggtcaga acgccattgg cattaaacac cagcttcatt gcaggctcgg ctgtacctgt
    32761 tggatcactg ctatcctggg tgtagacctg ccagttatta tccccggtct tcacaaagta
    32821 gacgctcatg tcatgagcat taccctgact gtcgaaaaca gtcaccgaac cttttttgtt
    32881 atagctatcc gcattgctgg cgctgaatgg cgtaacagta ggaagcggat cgctggaatt
    32941 caggttgatc tgcatcgatg ccgtggtggt agttttcgct gccatcaggg tattcgggat
    33001 cgaaatatta gtcggattcg ccccttgctg aatagtcggc ggcgtaccgg ttgccgggta
    33061 acccgtcagc tgtaaacctt gcatattcac caggttacgg ttttcatcca gcttaaattg
    33121 tccgttacgg ctgtagaaca ccgaaccgtt gctatctacc agacggaaaa aaccgttctg
    33181 gctgatagca acgtccagac cgcgcccggt gttggtggtc gtgccatctg taaagtcctg
    33241 agtgataccg gcaactttta cccccagtcc cactttcgaa ccggcaaaca tatcggcaaa
    33301 agaggccgtg cctgatttaa agccgtaggt ggcggagttg gcgatattgt tgccaataac
    33361 atcgaggtta gtggcggcag cgtttaatcc gctaaccgct tgagaaaagg ccatgactga
    33421 ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg tcgagggtgg tggtgccgta
    33481 agtgccgaga tccagcgtat taccgctgtt gccgcggatc acaccctgca ccagagcaaa
    33541 ctgcagcggc tgggcaacca gttgtgtacc accgttactg gcgctaatcg ctacattgta
    33601 agaaccgttc ggcgcagtgc tgccatcagt caacgtaccg tcccaggtga aactgtgaac
    33661 tccggcggtc agttcaccaa tatcaatggt gcgcacaacc gcgccatttt tatcggtgat
    33721 ggtggccgta actttgtctg ccgcctgttg cagctcaaca ccaaacggcg tggtcgtggt
    33781 cacagcccct tcttcactgc cggttcccgc aagaacagtt gtgccgggga tcatcacgcc
    33841 gtgaccgatc aggttactgg cctgtaacga ctggctgtta tcaatctgtc cggaaataga
    33901 tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg ctgatttgtg ccaattgcga
    33961 cgtcagctcg ttgttttcca ttggattggt cgggtcctgg tttttcagct gcgccaccag
    34021 caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg cccgtgagcg aactactgct
    34081 ggtggtactg acgccggtat ttgtcggatc ggtggtggtt accgcaatgg acatagcttt
    34141 ctcctttatt gaccgagcgt aagggttttc agcatcatgc ttttcaccgt gttgagcact
    34201 tcaacgttgg cctgatagct gcgtgacgcc gacatggtgt taaccatctc cccgacgaca
    34261 tcaacgttcg gcatttttac gtagcccttt gcatctgcca gcggattacc cggttcataa
    34321 accagtttgt ccggggcctg actttctata acatcggcaa cctttacgcc gcctgtcgca
    34381 gcacctggtg cagcgttaac ctggaatacc acctgttttg cccgatatgg ctgtccatca
    34441 ggaccggtca cgctatcagc atttgccaga ttactggccg ccacgttcag gcgctgggac
    34501 tgggcagtta acgccgaccc ggcgatatca aaaatattca gcagtgccat ccgttaattt
    34561 ccgctctgta aaacgttcat catgcctttg atttgcccgc tcaacgcgct aaggctcatc
    34621 tggtattgca ggctgttatc ggcaaactgg gtgcgttcgc gatccatatc gacggtatta
    34681 ccgtcaagcg aaggctggtc cggaatacgg tattgcagtt ctgcggtagg aggcgtcagc
    34741 gcctgcgccg ggatgtgttg cgttgaggtc atcgtcagtg caaccacact ggttgcatcc
    34801 cgtccacgtt gcatgacttt tttaagttca ctggcaaaat cgatatcgcg cgcctgataa
    34861 ccaggggtat cggcattggc gatgtttgct gccagcactt cctgacgctg ggcgcgcaga
    34921 ttgagcgcct cttgttgaaa acgtaaggcg gcgtcgagct tatcgagcat atctcctccg
    34981 caggtatcaa aattctgcca tcagcttaaa tgcctttacg cgcgcgttat cggcggaata
    35041 aacgcaaaat gggtcgctat ttatgccgtt gatggtcatt gcggacaggt acaattcacg
    35101 ttgtagaaat ggctgggggc gaaaatgctg acaataaaac gtagcgtggc gatcatcgcg
    35161 atactgttca gtccgttaag tacggcgagc aatctcacat cgcaattgca caactttttt
    35221 agcgcccaac tcgcgggggt aagtgatgag gttcgtgttt ctattcgtac agcgcccaat
    35281 ctactaccgc catgcgagca gccattgctt tcgatgagca ataattcccg cctgtggggc
    35341 aatgtgaatg tgttggcacg ctgcggtaac gacaaacgat atttacaggt taatgtgcag
    35401 gccacaggaa attatgtggt tgccgcgatg cccattgtgc ggggaggaaa gctggaagct
    35461 ggcaatgtca aactaaaacg cggacggctg gataccctgc caccgcgtac ggtgctggat
    35521 atcaatcaac ttgttgatgc cattagcctg cgcgatctat cacccgatca acctatccag
    35581 ttaacccagt ttcgccaggc atggcgggta aaagcgggac aacgcgtcaa tgtgatcgcc
    35641 agcggtgatg ggtttagcgc caacgcagaa ggtcaggcgc tgaacaatgc agccgtcgca
    35701 cagaatgcgc gggtgcgcat ggtatcggga caggtagtca gcggcgttgt tgatgcagat
    35761 gggaatattc ttataaacct gtaagctgtt aaagattacc cgtcccttgc cgataaataa
    35821 gcaacacatg ataaaagcgc cctcaatgag gaataaacca tgagtattga tcgcacttcg
    35881 cctctgaagc ctgtaagcac cgttcaaccg cgcgaaacca ctgacgcgcc ggtaacgaac
    35941 agccgggcgg caaaaacaac cgcctccacc agcaccagtg tgacgttaag cgacgcgcaa
    36001 gcaaaactga tgcaacccgg cagcagtgat atcaatcttg aacgtgtcga agcgttaaaa
    36061 ctggcgattc gtaacggtga actaaaaatg gacaccggca aaattgccga tgcgctgatc
    36121 aacgaagcgc agcaagactt gcagagtaac tgatcgtatg acacgtcttg cagagatcct
    36181 cgaccagatg tccgctgtgc ttaacgatct caaaacggta atggatcaag agcagcaaca
    36241 tctctctatg gggcagatca acggcagcca gttgcaatgg attacagaac aaaaaagctc
    36301 actgctggcg acgctggatt acctcgaaca gttacgcaag aaagaaccca atacagcaaa
    36361 tagcgttgat attagtcaac gctggcagga aattactgtg aaaacgcagc aactacgcca
    36421 aatgaatcaa cataacggct ggttactgga aggacagatt gagcgcaatc aacaggcgct
    36481 ggaaatgttg aaaccgcatc aggaaccgac gctatatggg gcgaacggtc agacctcaac
    36541 aacccatcgc ggcggtaaaa agatttcgat ctgaatatta tctccggcct gcactgcagg
    36601 ccggaatgca ttgttacacc gtccggcggg caaattcttt aactttgaag cccagtaccg
    36661 ccagtgcagc gaagtacgcg gcaatccccg ccagcacgac cgccattaaa cgcagtaagc
    36721 gccagggcat ggtacccaat gaccactccg gcatgatatg taacatacct aaaagcacgc
    36781 cagacatcac cagtaccgcc accaccagac gcaacagaaa cgccatccag ccgggttgcg
    36841 gagtaaagat tttctgctta cgcaactgcc agtaaagcag cgaagcattc agacacgccg
    36901 ccagaccaat agaaagtgac agcccggcat gtttcaacgg accaataaac gccaggttca
    36961 tcaattgcgt taaaatcagc gtaacgatgg caattttcac tggcgtttta atgtcctggc
    37021 gggaataaaa gccaggagcc aacactttca ctacaatcag gccaatcaaa cccaccgagt
    37081 aggcaattaa cgcccgctgg gtcatcagcg catcaaacgc ggtaaattta ccgtactgga
    37141 acagcgaaac ggttaacgga cctgaaagaa tgcccaacgc aaccgcactc ggcagcgcca
    37201 acaggaaaca aagacgcaat ccccagtcca tcaaacggtt gtattcatca tgattgccac
    37261 tggcaaaact tttcgacagc gacggcagca aaatggtgcc aagcgccacg cccagcacac
    37321 cggacggaaa ctccattaag cggtcggcgt aatacatcca agacaccgaa ccggaagcaa
    37381 gaaacgaggc aaaaatggtg ttgatgatta aggagatctg gctcacagag acgccaagga
    37441 tcgccggtcc catctgtttc accacgcgca ttgctccggc atcgtggaag ttaatgcgcg
    37501 gcaggaccag catgccgatc ttcttcaggt gcggtagctg atacaccagc tgcaggatgc
    37561 cgccgaccgt aacagcccac gccagcgcca gcaccggtgg gttaaagtac ggtgcggcaa
    37621 acagcgcgaa accaatcatg ctgatgttaa gcagtgttgg agcaaacgcc ggaatcgaga
    37681 agcggttcca cgtattcaga atcgctccca ccagcgacgc cagggagatc agcaagatat
    37741 agggaaaggt aatctttagt agctggctgg tcagggcaaa tttgtcagct gtgtcagcga
    37801 agcctggcgc ggtcaccatg atcacccacg gtgcggcgag catgccagcg accgtcacaa
    37861 ccgccagcgc gagtgtcagc aggccagaaa cataagagac aaagacccgc gtggcgtctt
    37921 caccctgctt acttttatat tccgccagaa tcggtacaaa tgcctgggaa aatgcccctt
    37981 cggcaaagat acggcgtaac aagttaggaa gtttaaaagc gacgaaaaag gcgtcggttg
    38041 ccatccctgc gccaaagatt ctggcgacaa ttgcgtctcg tgcgaagcca agcacacgcg
    38101 aaaacatggt catcgagctg acggcggcca gcgattttaa taaattcatc ggtgttctaa
    38161 tccagaccca cgggcaaaac gcctgcaagg caggcgctga aagaggcgaa tagtctacct
    38221 ggattatggt gaattgctac cgccagatgt tacagggttt attcactcat cgcatcgcgc
    38281 cagatcttgt caacgatacg ttgcgccagc acggcctgtt cgccggcggt ttgcggaact
    38341 gtctgatttt gcacacattc aatgaagtgc cgcgcacagc cgacaaatcc acgttgttca
    38401 agagtgctct gccaaccagg aatcggttta tgcactacgc cctgcccgcg ctcctcacgc
    38461 cattcgcgca tatccgtaat gtcgatgagc gcaccgtcag tcacggcctg cacggtttca
    38521 cgctgacttc cggcacggcg atgcatacag gtggtgatct gcaaaggacc ggccgaaaaa
    38581 tggtgctcgg caaacagcat ttcgccagcg tcgttagtca gtagcgtacc gccatccaga
    38641 gaggctttgc cgcccgacaa ccacagcgcg gtatccacca catgcagata atcatccagc
    38701 aacgtgaaat aaagatcgtg tggcccgaca ctattgctac gatgtttatc cattcttagc
    38761 gaagctgcgg tagcgagttg cgtttttaac tcaccgtaga gtggtgcgaa acgacggtta
    38821 aaaccgacca tcaacgtcag ttttttccgc gccgccagtt ccaccagccg ttcagcatcg
    38881 cgcagatttt ctgccagcgg tttatcgaca cagacatgta cacccgcatt gagtaacgta
    38941 ctgaccacgt caaagtggct ggcggtgctg gaatgcacaa aaaccgcatc gcaactggcg
    39001 gcaaggctgg ataacgaatc ggcataagga atgcgccagc tttcacaaat tggcagggct
    39061 ttcgcgcgcg taggcgacca ggctccttgt aacatccagt cagacgctgc cgccagcacc
    39121 ggtaaccacg ctttttgcgc aatgccacct aatcccacta cgccgatacg taattttttc
    39181 actttaatct cccagatgga ccagcaacga atcaagacgc tgtttcagtt ctgccacttc
    39241 gatttccagg gcttcgacgc gggcctgtaa atcaccatca accgcgttcg acatatccgt
    39301 caccgccggc tgatcttcaa cctcaccact gaaaagatgc atgtagcggc tttcgcgttt
    39361 acccggttcg cgggccagac gcaccacaaa aggaccatct tcgcgatttg ccagttgttc
    39421 cagcgtcaac tccacttccg ccatatcgct gaattcatac attcgcgcgg cgcggctgcg
    39481 cagttcaccc ggcgtctggg caccacgcaa taacaacgtg gtgattaacg ccacttctgc
    39541 ggcgctcagt ttcagatcgc caaattctga attacaaaaa cgttgctcat atttggtgac
    39601 ccgattacca aaaccgctca ccgtgcgtag ataatgacgt ttgaccagat tatccagctg
    39661 ttcctgcact tcagattcac tcagattcat gaccggttca cggttcgttt tctgattaca
    39721 ggccgttact acaccattga ctgagagcgg gtattgctcc ggcgtggtca cctgcttttc
    39781 cagcaagcag ccaatcacgc gggcttccag agcagtaagt tgatatttca tgagattctc
    39841 cttagcggcc gggcgtccag tctggggtag ttaatgccgt cagtacgtga tcgcgccatt
    39901 gtccatcaat caacagatag tctttcgcat agccttcttt ttcaaaaccc agtcgcgcca
    39961 gtaaatcacc gctgcgttta ttgtgcggca tataattagc cataatgcga tgaatatgtt
    40021 gggtgcgctg catataacga atggctgcgg tcagggcttc aaacatgagt cctttgccct
    40081 gccatttttg cccaatcgaa taaccgagat agcaggcatg aaaagagccg cgaacaacat
    40141 tggaaaaatt ggcaacgcca ataatctctt tttcgtccgg gtcgaataaa ccaaagtaga
    40201 aagctgaacc ttgtttatga aattcgttaa tcatccccag cctggcctgc cagcctgatg
    40261 gataacagtg gctttcgtcg cgcactggct cccagggctt gaggaaatgg cgattctctg
    40321 cgtaataatc cgcaagacgc caggcatcac gatcatgcac cagacgcacg accagtcggt
    40381 ctgtggttaa gcgcactttt ggcacgttac tgcgatagcc aaacattcta tacctactcc
    40441 ttcccgtaac gtctttaata cgctgtaagt attactatac ctgcgcaacc agaccctgtg
    40501 aaaagtccaa aatgccattt tctgcattat tcacatattt gataaaaact ctctatcaaa
    40561 gcgacgtttt gataaaaaaa tattgtcccc gaagtgatgg gaaaagtctt agcggcaatg
    40621 cagaatagaa agtgctcatc ttttatttcc cgggagggga aatgtcccgc gtgtcgcagg
    40681 cgaggaacct gggtaaatat ttcctgctca tcgataatat gctggtcgtg ctggggttct
    40741 ttgttgtctt cccgctgatc tctatccgct tcgttgatca aatgggctgg gccgccgtca
    40801 tggtcggtat tgctctcggt ctacgccagt ttattcagca aggtctgggt attttcggcg
    40861 gcgcaattgc cgaccgcttt ggtgccaaac cgatgattgt taccggtatg ctgatgcgcg
    40921 ctgccggatt cgccacaatg ggtatcgccc acgaaccgtg gctattgtgg ttttcatgcc
    40981 tgctctcggg actcggcggc acgttgtttg atccgccgcg ttcggcgctg gtggtgaaac
    41041 taatccgtcc acaacaacgt ggtcgttttt tctcgctgtt gatgatgcag gacagtgccg
    41101 gtgcggtcat tggcgcattg ttggggagct ggctgttgca atacgacttt cgcctggtct
    41161 gcgccacagg ggccgttttg tttgtgctgt gtgcggcgtt caatgcgtgg ttgttaccgg
    41221 catggaaact ctccaccgta cgcacgcccg ttcgcgaagg catgacccgc gtgatgcgtg
    41281 acaagcgttt tgtcacctat gtcctgacgc tggcgggtta ctacatgctg gctgtacaag
    41341 tgatgctgat gctgccaatt atggtcaacg acgtggctgg cgcaccctct gccgttaaat
    41401 ggatgtatgc cattgaagcg tgtctgtcgt taacgttgct ctaccctatc gcccgctgga
    41461 gtgaaaagca ttttcgtctg gaacaccggt tgatggctgg gctgttgata atgtcattaa
    41521 gcatgatgcc ggtgggcatg gtcagcggcc tgcaacaact tttcaccctg atttgtctgt
    41581 tttatatcgg gtcgatcatt gccgagcctg cgcgtgaaac cttaagtgct tcgctggcgg
    41641 acgcaagagc tcgcggcagc tatatggggt ttagccgtct gggtctggcg attggcggcg
    41701 ctattggtta tatcggtggt ggctggctgt ttgacctggg caaatcggcg caccagccag
    41761 agcttccgtg gatgatgctg ggaattattg gcatcttcac tttccttgcg ctgggttggc
    41821 agtttagtca gaaacgcgcc gcgcgtcgtt tgcttgaacg cgacgcctga tttgatactc
    41881 ccccctgact cctccatact gaaagtgaac tgacagccaa atggaggagt cacgtgaagc
    41941 tatacattta cgatcactgc ccttactgcc tcaaagcccg catgattttc ggcctgaaaa
    42001 atatccccgt cgaattacat gttctgctca acgacgacgc agaaacaccc acccggatgg
    42061 tcggtcaaaa acaggttccc attctgcaaa aagatgacag ccgctatatg ccagaaagca
    42121 tggacatcgt tcactatgtc gataaactcg acggcaaacc gttactgacc ggcaaacgtt
    42181 cccctgccat tgaagagtgg ctgcgcaagg tcaatggcta cgcaaacaaa ctgctgttgc
    42241 cgcgttttgc caaatcggca ttcgatgagt tttctactcc cgccgcgcgc aaatatttcg
    42301 tcgacaagaa agaggccagc gcgggtaatt ttgccgacct gctggcccac tctgacggtc
    42361 tgattaagaa tatcagcgat gatttacgtg cgctggacaa actgatcgtc aaaccgaacg
    42421 ccgtgaatgg cgaactttcg gaagatgata ttcagctatt tccgctactg cgtaatctga
    42481 cgctggtagc cggaatcaac tggccaagcc gcgttgctga ttaccgcgat aatatggcga
    42541 aacagacaca aatcaatttg ttatcatcaa tggcgattta atcctgaccc gccgcggcag
    42601 cctgtattcg cgggttgccg ccgttttcag ttttttctgc tggcgagttt atcgctggtg
    42661 acacatgctg cgttgtgtca ggatattgac gcagatttca tgtcgaggac taccatgaac
    42721 aagtttttat ttgccgctgc attgatcgtc agtggcctgc tcgttggctg taatcaactc
    42781 acccaataca ccatcaccga acaagaaatt aaccagtcgc ttgcgaaaca taataatttc
    42841 tcaaaagata tcggtttacc cggcgtggct gacgcccata ttgttctgac aaacttgacc
    42901 agccaaattg gtcgcgaaga gccgaataag gttaccctaa ccggagacgc caatctggac
    42961 atgaactccc tgttcggtag tcagaaagcg accatgaaac tgaagctgaa agcgctgccc
    43021 gtgtttgata aagaaaaagg tgcgatcttc ctgaaagaga tggaagtggt cgatgcgacg
    43081 gtacaaccgg aaaaaatgca aacggtgatg caaacgttgc ttccctattt gaaccaggca
    43141 ttacgcaatt actttaacca gcaacctgct tacgtcctgc gcgaagatgg cagtcagggc
    43201 gaagcaatgg cgaaaaaact ggcgaaaggc attgaagtga agccaggcga aattgtcatt
    43261 ccgtttacgg attaatgacg agggcgcatt cgcgcccttt atttttcgtg caaaggaaaa
    43321 cgtttccgct tatcctttgt gtccggcaaa aacatccctt cagccggagc atagagatta
    43381 atgactgcac catcccaggt attaaagatc cgccgcccag acgactggca ccttcacctc
    43441 cgcgatggcg acatgttaaa aactgtcgtg ccgtatacca gcgaaattta tggacgggct
    43501 atcgtaatgc ccaatctggc tccgcccgtg accacagttg aggctgccgt ggcgtatcgc
    43561 caacgtattc ttgatgccgt acctgccggg cacaatttca ccccattgat gacctgttat
    43621 ttaacagatt cgctggatcc taatgagctg gagcgcggat ttaacgaagg cgtgttcacc
    43681 gctgcaaaac tttatccggc aaacgcaacc actaactcca gccacggcgt gacgtcaatt
    43741 gacgcaatca tgccggtact tgagcgcatg gaaaaaatcg gtatgccgct actggtgcat
    43801 ggtgaagtga cacatgcaga tatcgacatt tttgatcgtg aagcgcgctt tatagaaagc
    43861 gtgatggaac ctctgcgcca gcgcctgact gcgctgaaag tcgtttttga gcacatcacc
    43921 accaaagatg ctgccgacta tgtccgtgac ggaaatgaac ggctggctgc caccatcact
    43981 ccgcagcatc tgatgtttaa ccgcaaccat atgctggttg gtggcgtgcg tccgcacctg
    44041 tattgtttac ccatcctcaa acgcaatatt caccaacagg cattgcgtga actggtcgcc
    44101 agcggtttta atcgagtatt cctcggtacg gattctgcgc cacatgcacg tcatcgcaaa
    44161 gagagcagtt gcggctgcgc gggctgcttc aacgccccaa ccgcgctggg cagttacgct
    44221 accgtctttg aagagatgaa tgctctgcag cactttgaag cattctgttc tgtaaacggc
    44281 ccgcagttct atggcttgcc ggtcaacgac acattcatcg aactggtacg tgaagagcaa
    44341 caggttgctg aaagcatcgc actgactgat gacaccctgg tgccattcct cgccggggaa
    44401 acggtacgct ggtccgttaa acaataaaaa tcgtagcttc ctgttgtcat taggttattt
    44461 tacctgtata aataaccagt atattcaaca gggggctatt atgcgaattg aagtcaccat
    44521 agcgaaaact tctccattgc cagctggggc tattgacgcc ctggctggcg aactttcccg
    44581 ccgtattcag tatgcgtttc ctgataatga aggccacgta tcggtacgtt atgccgcagc
    44641 gaataattta tcggttattg gcgcaacaaa agaagataaa cagcgcatta gcgaaattct
    44701 ccaggaaacg tgggaaagcg
//
LOCUS       DAPHDW010000022        44588 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.022, whole genome shotgun sequence.
ACCESSION   DAPHDW010000022 DAPHDW010000000
VERSION     DAPHDW010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44588
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.022"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(91..1293)
                     /gene="ubiI"
                     /locus_tag="RH057_001170"
                     /gene_synonym="visC"
     CDS             complement(91..1293)
                     /gene="ubiI"
                     /locus_tag="RH057_001170"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HDV3127576.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAADA
                     PPQLRVSAINAASEKLLTRLGVWQDILSRRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPAELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLVLVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGTNPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(1316..2494)
                     /gene="ubiH"
                     /locus_tag="RH057_001171"
                     /gene_synonym="visB"
     CDS             complement(1316..2494)
                     /gene="ubiH"
                     /locus_tag="RH057_001171"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HDV3127577.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADYATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVTLHCPDRVANVARTQSHVEVTLESGETLTGRVLVA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGKITHAGKRSAYPLALTRAARA
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            complement(2491..3816)
                     /gene="pepP"
                     /locus_tag="RH057_001172"
     CDS             complement(2491..3816)
                     /gene="pepP"
                     /locus_tag="RH057_001172"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HDV3127578.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENECEMRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     DVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            complement(3842..4420)
                     /gene="ygfB"
                     /locus_tag="RH057_001173"
     CDS             complement(3842..4420)
                     /gene="ygfB"
                     /locus_tag="RH057_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HDV3127579.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            4588..4917
                     /gene="zapA"
                     /locus_tag="RH057_001174"
     CDS             4588..4917
                     /gene="zapA"
                     /locus_tag="RH057_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HDV3127580.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            4959..5142
                     /gene="ssrS"
                     /locus_tag="RH057_001175"
     ncRNA           4959..5142
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="RH057_001175"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            5163..5765
                     /locus_tag="RH057_001176"
     CDS             5163..5765
                     /locus_tag="RH057_001176"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HDV3127581.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQQEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(5866..5925)
                     /locus_tag="RH057_001177"
     CDS             complement(5866..5925)
                     /locus_tag="RH057_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3127582.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(6258..6317)
                     /gene="ibsC"
                     /locus_tag="RH057_001178"
     CDS             complement(6258..6317)
                     /gene="ibsC"
                     /locus_tag="RH057_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HDV3127583.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            complement(6546..7778)
                     /gene="serA"
                     /locus_tag="RH057_001179"
     CDS             complement(6546..7778)
                     /gene="serA"
                     /locus_tag="RH057_001179"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HDV3127584.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            complement(8034..8693)
                     /gene="rpiA"
                     /locus_tag="RH057_001180"
     CDS             complement(8034..8693)
                     /gene="rpiA"
                     /locus_tag="RH057_001180"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HDV3127585.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            complement(8749..8934)
                     /locus_tag="RH057_001181"
     CDS             complement(8749..8934)
                     /locus_tag="RH057_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001328908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127586.1"
                     /translation="MHFAQRVRALVVLNGVALLPLFAPWSGIPRPLYALFAGRKGMPA
                     IARYFMDELTTRLANGV"
     gene            9076..9969
                     /gene="argP"
                     /locus_tag="RH057_001182"
     CDS             9076..9969
                     /gene="argP"
                     /locus_tag="RH057_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HDV3127587.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            10173..12317
                     /gene="scpA"
                     /locus_tag="RH057_001183"
     CDS             10173..12317
                     /gene="scpA"
                     /locus_tag="RH057_001183"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HDV3127588.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSFF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCRTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRGVLNLISQHHD"
     gene            12310..13305
                     /gene="meaB"
                     /locus_tag="RH057_001184"
     CDS             12310..13305
                     /gene="meaB"
                     /locus_tag="RH057_001184"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HDV3127589.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPYCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGVGQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRVLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            13316..14101
                     /gene="scpB"
                     /locus_tag="RH057_001185"
     CDS             13316..14101
                     /gene="scpB"
                     /locus_tag="RH057_001185"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HDV3127590.1"
                     /translation="MSYQYVNVVTINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            14125..15603
                     /gene="scpC"
                     /locus_tag="RH057_001186"
     CDS             14125..15603
                     /gene="scpC"
                     /locus_tag="RH057_001186"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
                     /protein_id="HDV3127591.1"
                     /translation="METQWTRMTANEAAEIIQHNDMVAFSGFTPAGSPKALPTAIARR
                     ANEQHEAKKPYQIRLLTGASISAAADDVLSDADAVSWRAPYQTSSGLRKKINQGAVSF
                     VDLHLSEVAQMVNYGFFGDIDVAVIEASALAPDGRVWLTSGIGNAPTWLLRAKKVIIE
                     LNNYHDPRVAELADIVIPGAPPRRNSVSIFHAMDRVGTRYVQIDPKKIVAVVETNLPD
                     AGNMLDKQNPMCQQIADNVVTFLLQEMAHGRIPPEFLPLQSGVGNINNAVMARLGENP
                     EIPPFMMYSEVLQESVVHLLETGKISGASASSLTISADSLRKIYDNMDYFASRIVLRP
                     QEISNNPEIIRRLGVIALNVGLEFDIYGHANSTHVAGVDLMNGIGGSGDFERNAYLSI
                     FMAPSIAKEGKISTVVPMCSHVDHSEHSVKVIITEQGIADLRGLSPLQRARTIIDNCA
                     HPMYRDYLHRYLENAPGGHIHHDLSHVFDLHRNLIATGSMLG"
     gene            complement(15600..16496)
                     /locus_tag="RH057_001187"
     CDS             complement(15600..16496)
                     /locus_tag="RH057_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417396.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3127592.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATMLQHIAEDRDGTNH"
     gene            complement(16663..17403)
                     /locus_tag="RH057_001188"
     CDS             complement(16663..17403)
                     /locus_tag="RH057_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HDV3127593.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            complement(17496..18131)
                     /gene="argO"
                     /locus_tag="RH057_001189"
     CDS             complement(17496..18131)
                     /gene="argO"
                     /locus_tag="RH057_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HDV3127594.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVAFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            complement(18270..19130)
                     /gene="mscS"
                     /locus_tag="RH057_001190"
     CDS             complement(18270..19130)
                     /gene="mscS"
                     /locus_tag="RH057_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HDV3127595.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            complement(19488..20567)
                     /gene="fbaA"
                     /locus_tag="RH057_001191"
     CDS             complement(19488..20567)
                     /gene="fbaA"
                     /locus_tag="RH057_001191"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HDV3127596.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            complement(20782..21945)
                     /gene="pgk"
                     /locus_tag="RH057_001192"
     CDS             complement(20782..21945)
                     /gene="pgk"
                     /locus_tag="RH057_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HDV3127597.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            complement(21995..23014)
                     /gene="epd"
                     /locus_tag="RH057_001193"
     CDS             complement(21995..23014)
                     /gene="epd"
                     /locus_tag="RH057_001193"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HDV3127598.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            23386..23817
                     /locus_tag="RH057_001194"
     CDS             23386..23817
                     /locus_tag="RH057_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HDV3127599.1"
                     /translation="MMTLPYPYSSSARFYALRLLPGQEVFSQLHAFAQQQQLHAAWIA
                     GCTGSLTDVALRYAGQEGTTLLNGTFEVISLNGTLEQSGEHLHLCVSDPHGTMLGGHM
                     MPGCTVRTTLELVIGSLEELAFSRQPCALSGYDELHISPVK"
     gene            23840..24418
                     /locus_tag="RH057_001195"
     CDS             23840..24418
                     /locus_tag="RH057_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HDV3127600.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVAGCLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            24419..25126
                     /locus_tag="RH057_001196"
     CDS             24419..25126
                     /locus_tag="RH057_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HDV3127601.1"
                     /translation="MHPFTSLTLWALVACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLRILAIVSTSQL
                     WMQYVPVQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRARGVPLDEG
                     WYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADSTLQRVARY
                     TMILLMLTEFGAWIWLR"
     gene            25114..25791
                     /locus_tag="RH057_001197"
     CDS             25114..25791
                     /locus_tag="RH057_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HDV3127602.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPGEITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVVIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            25785..26462
                     /locus_tag="RH057_001198"
     CDS             25785..26462
                     /locus_tag="RH057_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV3127603.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHSAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGLIRNVN
                     PPDDIHP"
     gene            complement(26434..27147)
                     /locus_tag="RH057_001199"
     CDS             complement(26434..27147)
                     /locus_tag="RH057_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HDV3127604.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKPLLHMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQTLPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPATALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRVLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(27144..27653)
                     /gene="fumE"
                     /locus_tag="RH057_001200"
     CDS             complement(27144..27653)
                     /gene="fumE"
                     /locus_tag="RH057_001200"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HDV3127605.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDMTWDFISNVNSITCNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            complement(27675..28640)
                     /gene="yggF"
                     /locus_tag="RH057_001201"
     CDS             complement(27675..28640)
                     /gene="yggF"
                     /locus_tag="RH057_001201"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV3127606.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCREMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            complement(28637..29914)
                     /locus_tag="RH057_001202"
     CDS             complement(28637..29914)
                     /locus_tag="RH057_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HDV3127607.1"
                     /translation="MKTKVAAIYGKRDVRLREFELPEITDNELLVSVISDSVCLSTWK
                     AALLGSEHKRVPDDLENHPVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSG
                     YSAGYSYEYFGGNATYMIIPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYH
                     TTQYVYEHRMGVKPGGNIALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQV
                     QKLLPVDLAASKGIELVYVNTKGMSDPVQTLRALTGDVGFDDIFVYAAVPAVVEMADE
                     LLAEDGCLNFFAGPTDKNFKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQL
                     QPSFMVTHIGGLDAVPDTVLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLLKE
                     LARLVEETHGIWNEQAEKYLLAQFGVDIGEAAQ"
     gene            complement(29929..31317)
                     /gene="cmtA"
                     /locus_tag="RH057_001203"
     CDS             complement(29929..31317)
                     /gene="cmtA"
                     /locus_tag="RH057_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HDV3127608.1"
                     /translation="MENKSARAKVQAFGGFLTAMVIPNIGAFIAWGFITALFIPTGWL
                     PNEHFAKIVGPMITYLLPVMIGSTGGHLVGGKRGAVMGGIGTIGVIVGAEIPMFLGSM
                     IMGPLGGLVIKYVDKALEKRIPAGFEMVINNFSLGIAGMLLCLLGFEVIGPAVLIANT
                     FVKECIEALVHAGYLPLLSVINEPAKVLFLNNAIDQGVYYPLGMQQASVNGKSIFFMV
                     ASNPGPGLGLLLAFTLFGKGMSKRSAPGAMIIHFLGGIHELYFPYVLMKPLTIIAMIA
                     GGMSGTWMFNLLDGGLVAGPSPGSIFAYLALTPKGSFLATIAGVTVGTLVSFAITSLI
                     LKMEKTVETESEDEFAQSANAVKAMKQEGAFSLSRVKRIAFVCDAGMGSSAMGATTFR
                     KRLEKAGLAIEVKHYAIENVPADADIVVTHASLEGRVKRVTDKPLILINNYIGDPKLD
                     TLFNQLTAEHKH"
     gene            complement(31345..31788)
                     /gene="cmtB"
                     /locus_tag="RH057_001204"
     CDS             complement(31345..31788)
                     /gene="cmtB"
                     /locus_tag="RH057_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IIA"
                     /protein_id="HDV3127609.1"
                     /translation="MRLSDYFPESSISVIHSAKDWQEAIDFSMVSLLDKNYISENYIQ
                     AIKDSTINNGPYYILAPGVAMPHARPECGALKTGMSLTLLEQGVYFPGNDEPIKLLIG
                     LSAADADSHIGAIQALSELLCEEEILEQLLTASSEKQLADIISRG"
     gene            complement(32102..34093)
                     /gene="tkt"
                     /locus_tag="RH057_001205"
     CDS             complement(32102..34093)
                     /gene="tkt"
                     /locus_tag="RH057_001205"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HDV3127610.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKELL"
     gene            34371..35129
                     /gene="loiP"
                     /locus_tag="RH057_001206"
                     /gene_synonym="yggG"
     CDS             34371..35129
                     /gene="loiP"
                     /locus_tag="RH057_001206"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HDV3127611.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLRQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRMSADGVK"
     gene            complement(35185..35928)
                     /locus_tag="RH057_001207"
     CDS             complement(35185..35928)
                     /locus_tag="RH057_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDV3127612.1"
                     /translation="MQKINFYRNRVAINVLAKDIANAREIFDAAEGHAVIGVLSAQFS
                     SVDEGIQEVKRWMAEIPSISVGLGAGDPAQYYKAAMIAAQIHPAHVNQTFTGCGFAAG
                     ALAATGGEQTHVNALVSPTGTPGEVLISTGVSSSQGTPARVSCDTAVRMMLDMGAHAA
                     KFFPMGGERSLPELYALATTAARNGMTLIEPTGGIDLDNFSVILKTCLEAGVPRIMPH
                     VYSSIIDPDTGYTRPDDVAVLLNKVKSLL"
     gene            complement(35915..37024)
                     /gene="dgaE"
                     /locus_tag="RH057_001208"
     CDS             complement(35915..37024)
                     /gene="dgaE"
                     /locus_tag="RH057_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glucosaminate-6-phosphate ammonia lyase"
                     /protein_id="HDV3127613.1"
                     /translation="MTQNIYQQLGLKQVINACGKMTILGVSSVAPEVMQATARAASSF
                     VEIDQLVDKTGELVSRFTGAEDSYVTSCASAGIAIAVAAAITRGDKARVALMPDSSGM
                     ANEVVMLRGHNVDYGASILSAIRLGGGRVVEVGSSNLAALWQLESAITDATAALLYVK
                     SHHCVQKGMLNIEDFAHVAQMYNLPLIVDAAAEEDLRAWVVSGADMVVYSGAKAFNAP
                     TSGFITGKKIWIAACKAQHHGIARAMKIGKENMVGLVYALENYHQGQAVITAEQLQPV
                     VEAISAIHGLTADIEQDEAGRAIWRIRIRVNAQELGVDARVVEAQLRGGDIAIYARRY
                     NLHQGVFSLDPRTVAEGEMALIVARLKEIADHAKD"
     gene            complement(37028..37888)
                     /locus_tag="RH057_001209"
     CDS             complement(37028..37888)
                     /locus_tag="RH057_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462668.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HDV3127614.1"
                     /translation="MMSDDVMQHELVERARESGTLTKADITKAWFIYWLGAEVSSSYE
                     RLQSLIFCASMTPIIKKLYPQKEEQVEALKRHLNFFNSEQTFGAVIQGISIAMEEQKT
                     RGEVINDASITGIKTGLMGPLAGMGDSIIWAAVMPLLIAIFIPFAANGSAMGGILPLI
                     LYPAITLAISYGMVHKGYTLGRDSIISLLQGGRIKELIYGANVLGLIMMGALSASFVK
                     ITSPLKISALKGSEVVVQQILDSIAPGLLPLAAVFAIYFYLVKKGPRYTTILLSIVAL
                     SVVCSLLGVL"
     gene            complement(37885..38634)
                     /locus_tag="RH057_001210"
     CDS             complement(37885..38634)
                     /locus_tag="RH057_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDV3127615.1"
                     /translation="MDTLLFASLMGLYYWFARLRLGYTFSAMLLQPVVVAVFVGLLLG
                     NMPAAMIIGAGMQLVYLGVTSTPGGNVPSDPALAACIAIPIAVKANMDPNLAIALAIP
                     FGVIGVFLDQLRRTLNAAWVHMADKHAETANMSAIMRCAFLYPALLGLVLRFPVVFAA
                     NYFGQNVVESFLKLMPHWLTHSFEIMGGILPALGFAITIMVIGKKSLLPWFIGGFFAV
                     LYLKVDIMAMAIFGTCVAFLIKGLAKNEGAA"
     gene            complement(38660..39145)
                     /locus_tag="RH057_001211"
     CDS             complement(38660..39145)
                     /locus_tag="RH057_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HDV3127616.1"
                     /translation="MANIVLCRIDSRLIHGQVVTKWVGQSQANRIAVVSDELDADPFM
                     KNIYLMAAPPNIKVDCFSNQSFAAAWKENQLGDGNVLVLFPSLAAIQEAVQQGFDVAD
                     IQVGGLGGGPNRKAVFQNITLDEKDVGILNDLKSRGIKAVFQTIPEDKPQSLDDILKK
                     F"
     gene            complement(39156..39584)
                     /locus_tag="RH057_001212"
     CDS             complement(39156..39584)
                     /locus_tag="RH057_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3127617.1"
                     /translation="MTTTVPIHPLPEILLLTHGGWGLQLCNSLRMVMGEIEGVTEIAL
                     MPVDTLGEFYQRVEAVVKTMPEGSLIVTDFIGGTTSNVAARLSADYPVAVISGLNASL
                     LLEALDKREHGPLTACVADLVEAGRNSCLDVVSHIRQLQQ"
     gene            complement(39703..42501)
                     /gene="dagR"
                     /locus_tag="RH057_001213"
     CDS             complement(39703..42501)
                     /gene="dagR"
                     /locus_tag="RH057_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator DagR"
                     /protein_id="HDV3127618.1"
                     /translation="MRRIDVIHKELERLTYGLELSDLAKEKAFTAEAIGFNLGLARNS
                     VSKDLNQLWNEGLVVKSQGRPVFFLHRHALELLINRKLDDCECIVHSVASLLPKKEKY
                     TDDDPFSGLIGYDRSLRDAVEKGRAAVLYPHGLHVLLTGASGVGKTFFAELMHCFACK
                     HTIGSPPPLVYFNCAEYAHNPELLSSHLFGHHKGAFTGASENKTGLVEQADGGYLLLD
                     EVHRLPYEGQEKLFSILDKGEYRPLGSSGPTYSISVRLICATTESVNSALLRTFQRRI
                     QVCIDLPGIRQRSVEEQLELIVSFFQRESRKIERTISLDKTLLHWLLSKPLEGNIGQL
                     KSDIQFLCAQAWASGMTERNDTLELDKRLAEMSFNTTPEQRLLVDALFGGKDRLNVDA
                     RTLPALKNSLATSAEIEESDLFYSFLTREYVNLRNSNVPPAETLAILKNKLSSIFEYG
                     LYSRHSAAHPPRYGDQIEERVTLLIGYVEQVLGFTLPENLANPLRKHFLALIGYVRRG
                     LIPQLYSSSLILDRCKDEYDNATLLCRKINELLHIQCPATEVVWLCLFLKECRHYRQR
                     IDTSPDCGVILIAHGATTATSQAQYVNRVLERDLFYAIDMPFEQSVHDTLETLTRMIQ
                     DRCWQRLILMVDIGSLVHFGSTISKLFQIDVLLLPNITLTSLLEVGLDLSYETGDLSQ
                     LAVLMQSKNIPCRLCTPQQESGGKVLVISCITGMGTAEKIKKVLEESFGELMSQDTRM
                     VILDYNEVRSLERIQQALNASERLAGIVGTFQPGLPDIPFISLEELFSEQGPELVLSL
                     LTPDLSSSERRLEMERSAMRFISALTMESIINHISVLNPQRILKEIEDVLNYLTNTLS
                     LKPSRQVTLRFLIHCCCMVERIVINRKPLQMALENRLDLDARAFSVIKSSFLPIEEAY
                     AIRLSDAEYFYIYELLYS"
     gene            complement(42760..43680)
                     /gene="speB"
                     /locus_tag="RH057_001214"
     CDS             complement(42760..43680)
                     /gene="speB"
                     /locus_tag="RH057_001214"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HDV3127619.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(43816..44547)
                     /locus_tag="RH057_001215"
     CDS             complement(43816..44547)
                     /locus_tag="RH057_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127620.1"
                     /translation="MKKWKVRSALVALIVLLAGCSSNAQYNSSASGNVGTAWGGDVHS
                     TVQGVSAERAWRDPAEMIVISYSTNVPSGYDRVYSIRINELEYAIRDGNFNSLPITRV
                     YDSSNNEPRYIVHARVGMNYQLYVRNYSRNTNYEIVATVDGLDVLNGKQGSLNNNGYI
                     VNAGDSLAIKGFRKDKHTEAAFQFANVVDSYAANSAQGDVRNTGVIGFAAFELQGPAQ
                     NALPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT        10807 a        12141 c        11120 g        10520 t
ORIGIN      
        1 tagtttattt caaatgagga aaatctcccg gcgaaaaaac cgggagatga aagtgtgatg
       61 ggtatcaaat aaacagcaga agagaaattt ttaacgcagc cattcaggca aatcgtttaa
      121 tcccattgcc tggcggataa gttgtggttt aacgccagga agcgtgtcgg ccagtttcaa
      181 accaatatca cgcagcagtt ttttcgccgg attggtaccg gaaaacagat cgcggaatcc
      241 ctgcatacca gccagcatca gcgccgcact gtgcttgcgg ctacgctcat accggcgcag
      301 gtaaatgtac tgcccgatgt ctttcccctg acgatgcaac cgtttcagtt cagcaatcag
      361 ctctgcagca tccataaagc cgagatttac tccctgcccc gccagcgggt gaatggtatg
      421 cgcggcgtcg cccaccagca ccagacggtg cgcggcaaac tggcgcgcat aacgccccgt
      481 cagtgggaac acctgacgcg cgctctcaac cttgcataag cccaggcgat tatcaaaagc
      541 gatatttaac gcgcgattaa attcatcttc acttgcctgc tgcatccgct gcgcttcctc
      601 tggcgacagt gaccagacaa ttgagcaaag atgcggatcg ctaagcggta aaaaggccag
      661 aatgccttcg ccatggaaaa cctgccgcgc caccgcatca tgcggttctt ccgtgcgaat
      721 ggtcgctacc agcgcgtgat gctgataatc ccagaaagtc agcggaatat ctgctttgtt
      781 gcgcaaccag gaattagcac cgtccgcgcc aatcaccaga cgcgccgtta acatgctgcc
      841 atctttcagc gtcaggaaag tttcattttc tccccaggcg acctgctgta attctgcggg
      901 ggccaacaga gtgatatctg acgactgctg cgctttgttc cacagcgcgt agtgaatcac
      961 tgagttttca acgatatgcc caagatggct atagcccatg ctttgatcgt caaacgaaat
     1021 gtgaccaaaa ctgtctttgt cccacacttc cataccgtga taacaactgg ccctacgaga
     1081 gagaatgtcc tgccagacgc caagacgggt gagtaatttt tcgctggcgg cattgatagc
     1141 cgaaacgcgc agttgtggtg gtgcatccgc cgccagaggt tccggtacgc gctgctccag
     1201 tacggcaacg cgtaagccgc tcccctgtaa gccgcaggca accgccagcc ccaccatgcc
     1261 gccaccaaca atggctacat caacactttg cattgtttat tccttaaaac cgccttcaac
     1321 gcgccaccca accgagggtg cgctgcgcca gcacatcgcg tgccggggtg aataattcca
     1381 tcgtcatcag tccgatgttg cgcccgacaa ccagcggtgc ccaacggttg gcaaaaagat
     1441 gtaccaggct gtccgtgacg ccgatggttg cttcgcgatc actctgtcga cgctgctgat
     1501 agcggcacaa tatgccgtaa tcaccgatgt cttctccgcg ctcatgcgcc tgagtcaggg
     1561 tttccgcaag gctcatgaca tctcgcatac cgaggttaaa cccttgcccg gcaatcgggt
     1621 gcagagtttg cgccgcattg cccaccagca cggtacgatg ggtaatagct ctggcggcgc
     1681 gggttaacgc cagcggataa gcactgcgtt taccggcgtg ggtaattttt ccaagtcgcc
     1741 agccaaaggc cgactggagt tcacggcaaa acttctcgtc gctccacgac agcacctctt
     1801 cgcgccgttc cagtggatga caccagacca gcgaacagcg tccgtcagac atcggcaaca
     1861 tcgccagcgg gccatgttgc gtgaagcgtt caaaagcgcg gccttcgtgc gcaacggaag
     1921 tcgcaacgtt ggcaatcacg gccagttgtt cgtaaggctc ctgctgccag tcaacgccgc
     1981 acgcggtggc taacgctgaa tgggtgccat cagctgctac cagcacgcgg cccgtcagcg
     2041 tctcgccact ctccagcgtc acttcaacgt gactctgagt acgggcaacg ttagccacgc
     2101 gatcagggca atgcagcgtt acgccaggtg ctttacgcag caatgcaaac agccgttgcc
     2161 cgacattgtg caattcgaca acctgtccca gcgccgccag ttggtaatct tctgcggcga
     2221 gggtgacaaa tccagcgtga ccacgatcgc tgacatgcac ggtagtgatg gcggttgcgt
     2281 aatccgccag agattgccag acgccgatgc gcgccagttg ctgacaggtc cccgcagcca
     2341 gcgcaatagc tcgtccatca aagcccggat gagcatgtga ctctggcgcg gtcgcttcaa
     2401 tcaaatgtac cggcagcgcc ccgtgactta accgggaaat agccagcgcc agcgtcgcgc
     2461 ccgccatgcc gccaccgacg atgattacgc tcattgcttt ctcgcagctg ccatcaacgc
     2521 ttcgatttct tccggctttt tcaccacgct ggcggtgagg ttttcgttgc cggtttcggt
     2581 aatcacaatg tcgtcttcaa tacgaatgcc gataccgcga tattgttctg gcacatccgc
     2641 atccggcgcg atatacagac caggctctac ggtcagtacc atccccggtt ccagaatgcg
     2701 cgagcgatcc tgaccataaa cgccaacgtc atggacatct agccctaacc agtggctaag
     2761 gccatgcata aagaaaggac gatgggcgtt ctgagcgatc agttcatcaa catcaccttt
     2821 cagaatgccg agttttacca ggccgctaac catgatgcgc accacttcac cagtgacttc
     2881 cagaatggaa gttcccggac gatacaggcg caagctggtt tcgagagact ccagcacaat
     2941 gtcgtagatt tcacgctggg cctgagtgaa tttgccgttg accgggaagg tgcgggtaat
     3001 atcgccagcg tatcctttgt attcgcaacc cgcgtcaatc aacaccaggt cgccgtcgcg
     3061 catttcacac tcgttttcgg tgtagtgcag aatgcagccg ttttcaccgc tgccgacaat
     3121 ggtgttatag gacggatagc gcgcaccgtg gcggttaaat tcgtggtgaa tttcgccttc
     3181 cagatggtac tcgaacattc ccggacggca tttttccatc gcccgggtat gtgccatggc
     3241 ggtgatttct cccgcgcggc ggagtacggc aatctcttct ggcgatttga acaggcgcat
     3301 ttcatgaaca acaggacgcc agtcgatcat cgttgccggt gcggtgagat tttgccgcga
     3361 acctttacgc agtttttcca gcgcactgtt cacgatttca tcagcatatg catattcgcc
     3421 ctgggcatgg taaaccacat ccaggccgtt aagtagttga taaagttgct gattgatttc
     3481 gctgaatgcc agtgcgcggt caacgcccag tttctctggc gcggcatcct ggcctaagcg
     3541 acggccaaac cagatctccg ccgtcaggtc gcgaacgcgg ttaaacagaa cgctgtggtt
     3601 atgagtgtca tcgcttttaa tcagcaccag caccgcttcc ggttcgttaa agccggtgaa
     3661 gtaccagaag tcactgttct gacgataggg gtattcgctg tcggcgctac gtgttacttc
     3721 tggtgcagca aaaatcagcg cggcgctgcc gggttgcatt tgctccacca gggcctgacg
     3781 gcgacgctga aactcttgcc gggatatctc actcataaca ctctccttac gttttttgtt
     3841 tttagtgtag agtcggtttt tgtacttctg gcgcggtcgg ttgcggatga gtaaaggtgt
     3901 cgtggcataa cagcgcggca acacgaacgt attcgatgat ctcttcaagc gacatttcaa
     3961 gctcttcctg atcttcgtct tcgtcgtaac ccagttgcgc aatgttacgc agatcgtcga
     4021 tagcttcacc ggtttcgccg gtcactttat ccagcttcgg ttgcgtaacg ccaagaccaa
     4081 gcaggaagtg attgacccaa cctgccaatg catcagcccg atcgaaaacg ctgacatcat
     4141 cgccatcagg cagataaagc tgaaaaagga agccgtcatc ctgcagggca tcgctggtgg
     4201 cagagtgcat tttacgcagt gcctgtgcca gctcatgacc gaaagccatg ccttcgttcg
     4261 tcaggtcgtg aagtagcggt agccatgagc tgtcatcgtt accgccacat atcatcccac
     4321 tgattaaacc atgcatctca gcaggggtca gacccgtccc ttgttggttc agatactggt
     4381 tcatttcgtt gtaaccaggc atttcgttct gtatagacat aagcattcgt cgtcaaaggg
     4441 aggaatattc atgatatgct accactttgg gccctggtgg accagaaaag ggcttgtctc
     4501 ttctcatcag ggtagctata gtgtcgcccc ttcgcagacc atgggtctaa agacgaaggc
     4561 agcgcagtca atcagcagga aggtggcatg tctgcacaac ccgtcgatat ccaaattttt
     4621 ggccgttcac tgcgtgtgaa ctgcccgcct gaccaaaggg atgcgttgaa tcaggcagcg
     4681 gacgatctga accaacggtt gcaagatctg aaagaacgca ctagagtcac aaatactgaa
     4741 cagttggtct tcattgccgc attgaatatc agctatgagt tagcgcaaga aaaagcgaag
     4801 actcgtgact acgcggcaag tatggaacag cgtattcgga tgctgcagca gaccatagaa
     4861 caagcgttac ttgaacaagg tcgcatcacc gaaaaaacta accaaaactt tgaatgacac
     4921 ttttcggttt actgtggtag agtaaccgtg aagacaaaat ttctctgaga tgttcgcaag
     4981 cgggccagtc ccctgagccg atatttcata ccacaagaat gtggcgctcc gcggttggtg
     5041 agcatgctcg gtccgtccga gaagccttaa aactgcgacg acacattcac cttgaaccaa
     5101 gggttcaagg gttacagcct gcggcggcat ctcggagatt cccttcttat ctggcaccag
     5161 ccatgacgca actaccagaa ctcccactga cattatcccg acaagaaatc cgcaaaatga
     5221 ttcggcaacg tcgtcgtgcg ttaacgccgg aacaacagca ggaaatgggt caacaagccg
     5281 ctacccggat gatgacttat cccccggtgg tgatggcgca tacggtcgct gtattcctct
     5341 cttttgatgg tgaactcgac acccagccgc taatagaaca actctggcgc gccggtaagc
     5401 gcgtatatct tccagttttg catcccttta gtgccggtaa tttgctgttc ctgaattacc
     5461 atccgcaaag cgaactggtg atgaacaggt tgaagatcca tgagccaaaa ctggatgtgc
     5521 gtgacgtgct acccctttcc cgattagacg tgctgatcac accgctggtc gcctttgatg
     5581 agtacggtca gcgcctggga atgggcggtg gtttttatga tcggacctta caaaactggc
     5641 agcactataa aacgcaaccg gtgggttatg cgcatgattg tcagttggtg gaaaaactcc
     5701 ccgttgaaga gtgggatatc cctcttcctg cggtggttac accgtcgaaa gtctgggagt
     5761 ggtaaggtcg atacacctgc atcgccctga ttgacatcgt tgattcttta acctaattta
     5821 gtgagtaagg gtaagggagg attgctcctc ccctgtgact gactgttaat aagctgggaa
     5881 acttatgagt aacagtacaa tcagtatgat ggcaagtcgc atcataaccc ttctccttca
     5941 agccctcgct tcggtgaggg ctttaccgtt acagccccat gctgcccagc tatcgtaagc
     6001 tctctttaaa taaccacaac gcattgatct acctttaaat cttttcttcg agcgtgctcg
     6061 aaaaacccca tgcaaaacag agataatttt tgggaaaaag gatctattac ggcagtaaac
     6121 gtgggcgatg aagtggctat caaaaagttc ggttcagggc gatgcacccc gcatcgccct
     6181 gattgacatc gttgattctt tgacctaatt tagtgagtaa gggtaaggga ggatgactcc
     6241 tcccctgaga ctgactgtta ataagctccg aaacttatca gtaacagtac aatcagtatg
     6301 atgacaagtc gcatcataac ccttctcctt taagccctcg cttcggtgag ggctttaccg
     6361 ttacagcccc atgctgtccc gccatcgtaa atccccatta aataaacaca acgcattgat
     6421 ctgactttga tttattttct ggagcagact cgcaaagtag aatgcgcaac gcggcaacgg
     6481 tgtggagaag ggataaaaaa acgggcaagt cagtgacctg cccgttaatt ttcagagaag
     6541 gggaattagt acagcagacg ggcgcgaatg gtacccggaa tagctttcat tgcctgcagc
     6601 gctttttcgg caacgtcttc gtcagcttca atatcaataa ctacataacc catctgggcg
     6661 gaagtttgta gatattgcgc ggcgatgttg acgccctgct cggcgaagat tttgttcagc
     6721 gcagttagca cgcccggacg gttttcgtgg atgtgcatca gacgacgacc accatgcagt
     6781 ggcagcgaga cttccgggaa gttcaccgca gagagcgttg agccattgtc agaatacttg
     6841 atcaatttac ccgcaacttc caggccgata ttctcctgcg cttcctgagt cgaaccgcca
     6901 atgtgtggcg tcagaaggac gttgtcgaat tcacacagcg gagaggtaaa tggatcgcta
     6961 ttggtcgccg gttccgtcgg gaatacgtcg attgccgccc ccgccagatg tttgctcgcc
     7021 agcgcatcac acagcgccgg aatatccacc acagtaccgc gcgaagcatt aatcagcagc
     7081 gagccgggct tcattagtga aatttctttc gcgcccatca tatttttggt ggacggattc
     7141 tctggtacat gcagactcac cacatcgctc atattcagca ggtcagaaag atgctgtacc
     7201 tgagtggcgt tgcccagcgg cagtttattt tcaatatcat aaaagtaaac atacattccc
     7261 agcgattcag ccagaatgcc caattgcgta ccaatatgac cgtagccgat gatacccagc
     7321 tttttgccgc gcgcttcaaa agaacccgcc gccagtttgt tccacacgcc acggtgcgct
     7381 ttagcattgg cttccggcac gccgcgcaat agcagcagca gttcgccaat caccagctcc
     7441 gcaacagagc gcgtatttga gaacggtgcg ttaaataccg ggatcccgcg ctttgccgcc
     7501 gcatccagat caacctggtt tgttccgata cagaaacagc caatagcgac cagtttttct
     7561 gcggcgttga tcacgtcttc agtcagatgg gtacgggatc gcaggccgat gaagtgggca
     7621 tcgcggatgg attcttttaa ttgttcatca tccagcgcgc ctttgtgaaa ttcgatgttg
     7681 gtgtaaccag ctgcacgaag gctttccagc gccttttggt gcacgccttc taccagcaga
     7741 aacttaatct tgtctttctc cagcgatacc tttgccattt acccaatcct gtcttttgaa
     7801 atgttgtgtg cggatttgcg tccgcctttc aacatatcaa aaaataatat tgcggcaata
     7861 tgaacgtttg cgtcgcgatg ttgaggaaat atcacgcaaa ggtaattctt ggatgaatat
     7921 gctggtttag aagatttatt tagagcaatc gacaatagcc aggtaaaaac gtgacacatg
     7981 tcaccaaatt taatgaagag aattttttta acgggggagg ttcccccgtc agatcatttc
     8041 acaatggttt tgacaccgtc aggtgtgcca atcagcgcaa cgtccgcgcc acggttagca
     8101 aacaagccaa cagtcaccac gccaggaatc gcatttatgg cgttttccat cgctatcggg
     8161 tcaaggattt ccatgccgtg gacgtcgagg atcacgttgc cattatcggt caccacgccc
     8221 tgacggtatt ccggacgacc gcccagtttc accagctgac gcgccactgc actacgtgcc
     8281 atcgggataa cttctactgg cagcgggaat ttacccagaa tatcaacctg cttggaagcg
     8341 tctgcaatac agataaattt ttctgcaacc gaagcaatga ttttttcacg ggtcagcgcc
     8401 gcgccgccgc ctttgatcat ttgcatgtgg ccgttgattt catctgcgcc atcaacgtag
     8461 atgccaaggc tgtcgacttc gttgagatca aaaacgtgaa tgccgaggct tttcagtttt
     8521 tcagtggaag catctgaact ggaaacggcc ccttcaatct ggcctttcat tgtaccgagc
     8581 gcgtcaataa agtgtgcggc ggtggaacct gtacctacac caacaatggt gccgggctga
     8641 acatactgaa gtgccgccca tcctactgct tttttcaatt catcctgcgt catgatcgtt
     8701 tcgcctgtgg tatgaaattt cacacgcatt atatacaaaa aaagcgattc agaccccgtt
     8761 ggcaagccgc gtggttaact catccataaa atatcgcgca atggcgggca tcccctttcg
     8821 ccccgcaaat aaagcataca acggtctggg tatgccgctc cacggtgcga acagaggcaa
     8881 taacgccacg ccattcaaca caaccagggc gcgaactcgc tgagcgaaat gcattagcgc
     8941 aaatccctga tcttttaagc tgcgcgttta accacgaata caaatgttac ccgccgacgg
     9001 cttcggtata tgcaacctga cacaaaattg tgtcatagtg caggaaaaag catttaccag
     9061 gagcagacaa cagcaatgaa acgcccggac tacagaacat tacaggcact ggatgcggtg
     9121 atacgtgaac gaggatttga gcgcgcggca caaaagctgt gcattacaca atcagccgtc
     9181 tcacagcgca ttaagcaact ggaaaatatg ttcgggcagc cgctgttggt gcgtaccgta
     9241 ccgccgcgcc cgacggaaca agggcaaaaa ctgctggcac tgctgcgcca ggtggagttg
     9301 ctggaagaag agtggctggg cgatgaacaa accggttcga ctccgctgct gctttcactg
     9361 gcggtcaacg ccgacagtct ggcgacgtgg ttgcttcctg cactggctcc tgtgttggct
     9421 gattcgccta tccgcctcaa cttgcaggta gaagatgaaa cccgcactca ggaacgtctg
     9481 cgccgcggcg aagtggtcgg cgcggtgagt attcaacatc aggcgctgcc gagctgtctt
     9541 gtcgataaac ttggtgcgct cgactatctg ttcgtcagct caaaaccctt tgccgaaaaa
     9601 tatttcccta acggcgtaac gcgttcggca ttactgaaag cgccagtggt cgcgtttgac
     9661 catcttgacg atatgcacca ggcctttttg cagcaaaact tcgatctgcc tccaggcagc
     9721 gtgccctgcc atatcgttaa ttcttcagaa gcgttcgtac aacttgctcg ccagggcacc
     9781 acctgctgta tgatcccgca cctgcaaatc gagaaagaac tggccagcgg tgaactgatt
     9841 gacttaacgc cggggctatt tcaacgccgg atgctctact ggcaccgctt tgctcctgaa
     9901 agccgcatga tgcgtaaagt cactgatgcg ttgctcgatt atggtcacaa agtccttcgt
     9961 caggattaat ccatcaaata atgcctgata gcacatatca ggcgttatcc tcacttcttt
    10021 ttgtattcct tgaatcacat cacaaaatag acaaatctca ggcggcaaaa aacgacgtct
    10081 gaatgcattt tttttgctgg cgacaaaccc acgtaaaaag ctcaccgtaa gcgcaaatac
    10141 cctcattttg attgcgtttt acggagcaaa taatgtctaa cgagcaggag tggcaacagc
    10201 ttgccaacaa ggaattgagc cgtcgggaga aaactgtcga ctcgctggtt cagcaaaccg
    10261 cggaagggat cgccatcaag ccgctgtata ccgaagccga tctcgataat ctggaggtga
    10321 caggtaccct tcctggtttg ccgccctacg ttcgtggccc gcgtgccact atgtataccg
    10381 cccaaccgtg gaccatccgt cagtatgctg gtttttcaac agcaaaagag tccaacgctt
    10441 tttatcgccg taacctggcc gccgggcaaa aaggtctttc cgttgcgttt gaccttgcca
    10501 cccaccgtgg ctacgactcc gataacccac gcgtggcggg tgacgtcggc aaagcgggcg
    10561 tcgctatcga caccgtggaa gatatgaaag tcctgttcga ccagatcccg ctggataaaa
    10621 tgtcggtttc gatgaccatg aatggcgcag tgctaccagt actggcgttt tatatcgtcg
    10681 ccgcagaaga gcaaggtgtt acacctgata aactgaccgg caccattcag aacgatatcc
    10741 tcaaagagta cctctgccgc aacacctata tttacccgcc aaaaccgtca atgcgcatta
    10801 tcgccgacat catcgcctgg tgttccggca acatgccgcg atttaatacc atcagtatca
    10861 gcggttacca catgggtgaa gcgggtgcca actgcgtgca gcaggtagca tttacgctcg
    10921 ctgatgggat tgagtacatc aaagcagcaa tctctgccgg actgaaaatt gatgacttcg
    10981 ctcctcgcct gtcgttcttc ttcggtatcg gcatggatct gtttatgaac gtcgccatgt
    11041 tgcgtgcggc acgttattta tggagcgaag cggtcagtgg atttggcgca caggatccga
    11101 aatcactggc gctgcgtacc cactgccaga cctcaggctg gagcctgact gaacaggatc
    11161 cgtataacaa cgttatccgc accaccattg aagcgctggc tgcgacgctg ggcggtactc
    11221 agtcactgca taccaacgcc tttgacgaag cgcttggttt gcctaccgat ttctcagcac
    11281 gcattgcccg caacacccag atcatcatcc aggaagaatc agaactctgc cgcaccgtcg
    11341 atccactggc cggatcctat tacattgagt cgctgaccga tcaaatcgtc aaacaagcca
    11401 gagctattat ccaacagatc gacgaagccg gtggcatggc gaaagcgatc gaagcaggtc
    11461 tgccaaaacg aatgatcgaa gaggcctcag cgcgcgaaca gtcgctgatc gaccagggca
    11521 agcgtgtcat cgttggtgtc aacaagtaca aactggatca cgaagacgaa accgatgtac
    11581 ttgagatcga caacgtgatg gtgcgtaacg agcaaattgc ttcgctggaa cgcattcgcg
    11641 ccacccgtga tgatgccgcc gtaaccgccg cgttgaacgc cctgactcac gccgcacagc
    11701 ataacgaaaa cctgctggct gccgctgtta atgccgctcg cgttcgcgcc accctgggtg
    11761 aaatttccga tgcgctggaa gtcgctttcg accgttatct ggtgccaagc cagtgtgtta
    11821 ccggcgtgat tgcgcaaagc tatcatcagt ctgagaaatc ggcctccgag ttcgatgcca
    11881 ttgttgcgca aacggagcag ttccttgccg acaatggtcg tcgcccgcgc attctgatcg
    11941 ctaagatggg ccaggatgga cacgatcgcg gcgcgaaagt gatcgccagc gcctattccg
    12001 atctcggttt cgacgtagat ttaagcccga tgttctctac acctgaagag atcgcccgcc
    12061 tggccgtaga aaacgacgtt cacgtagtgg gcgcatcctc actggctgcc ggtcataaaa
    12121 cgctgatccc ggaactggtc gaagcgctga aaaaatgggg acgcgaagat atctgcgtag
    12181 tcgccggtgg cgtcattcca ccgcaggatt acgccttcct gcaagagcgc ggcgtggcgg
    12241 cgatttatgg tccaggtaca cctatgctcg acagcgtgcg cggcgtactg aatctgataa
    12301 gccagcatca tgattaatga agccacgctg gcagaaagta ttcgccgctt acgtcagggt
    12361 gagcgtgcca cactcgccca ggccatgacg ctggtggaaa gccgtcaccc gcgtcatcag
    12421 gcactaagta cgcagctgct tgatgccatt atgccgtact gcggtaacgc cctgcgactg
    12481 ggcgttaccg gcacacccgg cgcggggaaa agtacctttc ttgaagcctt tggcatgttg
    12541 ttgattcgtg agggattaaa ggtcgcggtt attgcggtcg atcccagcag cccggtcact
    12601 ggcggtagca ttctcgggga taaaacccgc atgaatgacc tggcgcgtgc cgaagcggcg
    12661 tttattcgcc cggtaccatc ctccggtcat ctgggcggtg ccagtcagcg agcgcgggaa
    12721 ttaatgctgt tatgcgaagc ggcgggttat gacgtagtga ttgtcgaaac ggttggcgtc
    12781 gggcagtcgg aaacggaagt cgcccgcatg gtggactgtt ttatctcgtt gcaaattgcc
    12841 ggtggcggcg atgatctgca gggcattaaa aaagggctga tggaagtggc tgatctgctc
    12901 gttatcaaca aagacgatgg cgataaccat accaatgtcg ccattgcccg gcatatgtac
    12961 gagagtgccc tgcatattct gcgacgtaaa tacgacgaat ggcagccacg ggttctgact
    13021 tgtagcgcac tggaaaaacg tggaatcgat gagatctggc acgccatcat cgacttcaaa
    13081 accgcgctaa ctgccagtgg tcgtttacaa caagtgcggc aacaacaatc ggtggaatgg
    13141 ctgcgtaagc agaccgaaga agaagtactg aatcacctgt tcgcgaatga agatttcgat
    13201 cgctattacc gccagacgct tttagcggtc aaaaacaata cgctctcacc gcgcaccggc
    13261 ctgcggcagc tcagtgaatt tatccagacg caatattttg attaaaggaa tttttatgtc
    13321 ttatcagtat gttaacgttg tcactatcaa caaagtggcg gtcattgagt ttaactatgg
    13381 ccgcaaactt aatgccttaa gtaaagtctt tattgatgat cttatgcagg cattaagcga
    13441 tctcaaccgg ccggaaattc gctgtatcat tttgcgcgcg ccgagtggat ccaaagtctt
    13501 ctccgcaggc cacgatattc acgaactgcc gtctggcggt cgcgatccgc tctcctatga
    13561 tgatccattg cgtcaaatca cccgcatgat ccaaaaattc ccgaaaccga tcatttcgat
    13621 ggtggaaggt agtgtttggg gtggcgcatt tgaaatgatc atgagttccg atctgattat
    13681 cgccgccagt acctcaacct tctcaatgac gcctgtaaac ctcggcgtcc cgtataacct
    13741 ggtcggcatt cacaacctga cccgcgacgc gggcttccac attgtcaaag aactgatttt
    13801 taccgcttcg ccaatcaccg cccagcgcgc gttggctgtc ggcatcctca accatgttgt
    13861 ggaagtggaa gaactggaag atttcacctt acaaatggcg caccacatct ctgagaaagc
    13921 gccgttagcc attgctgtta tcaaagaaga gctgcgtgta ctgggcgaag cgcacaccat
    13981 gaactccgat gaatttgaac gtattcaggg gatgcgccgc gcggtttatg acagcgaaga
    14041 ttaccaggaa gggatgaacg ctttcctcga aaaacgtaaa cctaatttcg ttggtcatta
    14101 atccctgcga acgaaggagt aaaaatggaa actcagtgga caaggatgac cgccaatgaa
    14161 gcggcagaaa ttatccagca taacgacatg gtggcattta gcggctttac cccggcgggt
    14221 tcgccgaaag ccctacccac cgcgattgcc cgcagagcta acgaacagca tgaggccaaa
    14281 aagccgtatc aaattcgcct tctgacgggt gcgtcaatca gcgccgccgc tgacgatgta
    14341 ctttctgacg ccgatgctgt ttcctggcgt gcgccatatc aaacatcgtc cggtttacgt
    14401 aaaaagatca atcagggcgc ggtgagtttc gttgacctgc atttgagcga agtggcgcaa
    14461 atggtcaatt acggtttctt cggcgacatt gatgttgccg tcattgaagc atcggcactg
    14521 gcaccggatg gtcgagtctg gttaaccagc gggatcggta atgcgccgac ctggctgctg
    14581 cgagcgaaga aagtgatcat tgaactcaat aactatcacg atccgcgcgt tgcagaactg
    14641 gcggatattg tgatccctgg cgcgccaccg cggcgcaata gcgtgtcgat cttccatgca
    14701 atggatcgcg tcggtacccg ctatgtgcaa atcgatccga aaaagattgt cgccgtcgtg
    14761 gaaaccaact tgcccgacgc cggtaatatg ctggataagc aaaatcccat gtgccagcag
    14821 attgccgata acgtggtcac gttcttattg caggaaatgg cgcatgggcg tattccgccg
    14881 gaatttctgc cgctgcaaag tggcgtgggc aatatcaata atgcggtaat ggcgcgtctg
    14941 ggggaaaacc cggaaattcc tccgtttatg atgtattcgg aagtgctaca ggaatcggtg
    15001 gtgcatttac tggaaaccgg caaaatcagc ggggccagcg cctccagcct gacaatctcg
    15061 gccgattccc tgcgcaagat ttacgacaat atggattact ttgccagccg cattgtgttg
    15121 cgtccgcagg agatttccaa taacccggaa atcatccgtc gtctgggcgt catcgctctg
    15181 aacgtcggcc tggagtttga tatttacggg catgccaact caacgcacgt agccggggtc
    15241 gatctgatga acggcatcgg cggcagcggt gattttgaac gcaacgcgta tctgtcgatc
    15301 tttatggctc cgtcgattgc taaagaaggc aagatctcaa ccgtcgtgcc aatgtgcagc
    15361 catgttgatc acagcgaaca cagcgtcaaa gtgatcatca ccgaacaagg gatcgccgat
    15421 ctgcgcggtc tttccccgct tcaacgcgcc cgcactatca ttgataattg tgcacatcct
    15481 atgtatcggg attatctgca tcgctatctg gaaaatgcgc ccggcggaca tattcaccac
    15541 gatcttagcc acgtcttcga cttacaccgt aatttaattg caaccggctc gatgctgggt
    15601 taatggtttg tcccgtcacg atcttcagca atatgctgaa gcatcgtggc ggtataacga
    15661 tgatttctga ttgaataaag atattcctgc atatacatcg gcctgtgcgg cgcatcaaaa
    15721 tatttcagcg ttgccggatt caccagtcgc caggcaaaat gcgggatcac cgtcagaaaa
    15781 tgtccgcgct ccacggcgct gattttggcc ataaaactgt agggacgata aataatggtt
    15841 ggattgatcc cacaaggtcg catattcgca tcaatcattg cttcgaagtt ggcccggttc
    15901 tgaaagcgca tttgtagcca gggcagctcc tgaagcagct cttgctcttg acgctcttca
    15961 taacggcgag aaacgagaaa gcctaatcgc agcggtggta gttcactgat agtcaggttt
    16021 tccagttcct gaactcgtgc agatacatgc tgtggggaga taataaaatc taactggcgg
    16081 tcaaaaagat tatcgataac gccgttttcg ctgaactcaa ctggctgggc ggtcacgcct
    16141 tcatacttat ctccgagact aatcaactga tcgaaaataa ttgtcggata agtattatct
    16201 atgccaatca caatttctcg tgaacgacgt cctgagttat ggatttcatt atcaatcgca
    16261 gaaagccgtt gataaaccgg gaacagtttt tgatacagtt cctgtccggc tttattgagg
    16321 ctgatattat tatctttgcg ggtaaatagc gtatagccaa tttgttcttc cagcgcggca
    16381 atgcttttac caaatggcga agccgtcata tggatttttt ctgccgcccg ggcaatatta
    16441 ttggtttgcg ctaataaaat gaaattgcgc atctttttcg aaataaaaat gtccatcccc
    16501 ctcccccgca tccttgtccg cttaaccgtt tcagtcagcc tatccttgat gaaaccgcga
    16561 gcaaagatag gtgattacgt catggtttta cagaaaatta cagaaaaagg aggcaatatc
    16621 gggtaaaggc attagcccga cgaatacgtc gggctacaag tattattgtg ctgcaggtgt
    16681 tttagcgggt tgttgatcca caggttctaa ctggaagacc acatcgacct gatcatcaaa
    16741 ctgaatagcg gcctgctcgt aagtttcctg ggcggacacc ggcgcggcat cggctttcat
    16801 catccgcacc attgggctgg gctgatagtt ggaaacatgg tagcgcacgc tatataccgg
    16861 ccccagttta cgatgaaagc cgttcgccag ttcctgcgcc tgatgaatcg cgttatcaat
    16921 cgctgcctta cgcgctttgt ctttataggc atccggctgc gccacgccca gcgacacaga
    16981 acgaatttcg ttaagacccg ccttcagcgc gccatccagc aaggaattca gtttgtctaa
    17041 ctgacggagc gtgacttcca ccgttctcac agcgcggtag cctttcagga tacttttacc
    17101 atcctgataa tcataatctg gctgggtgcg taagttcgct gagctgatat cttttttcgc
    17161 gatctgattg agttcaagga aggaaatgta ttgtgcgacg cgctcatctg cctgtttctt
    17221 ggcagtagcg gcatccttcg cggccacgtt aacttcaatc gcaagagtgg caatgtctgg
    17281 caccgcatcc acgcttgccg taccggaggt gacaatatgc ggtccatccg gcaattcgtt
    17341 agcctgcgct gccatcccgc taatacccat taatgccgcc agggcgataa ctttgaactt
    17401 cacgttagtc tctccgtgtt aattgacttg cccaaaattc aggacctctg gaggcaagct
    17461 tagcgcctct gttttatttt tccatcagat agcgcctaac tgaacaaggc ttgtgcatga
    17521 gcaataccgt ctctcgccag ctgcaaggct ataaaccaca tgacacatcc cactaccaga
    17581 ttgataatgc gctgtgattt tgccgtgcgc aggcgcggtg ccagccaggc tgcgagaata
    17641 gccagaccaa agaaccacag gaaagaggcg ctaattgtcc cgagcgcaaa ccaacgtttt
    17701 ggttcgacgt caagctgccc gccaaggctg cccagtacaa caaaagtatc caggtaaaca
    17761 tgcggattca gccaggtcac tgccaacatg gtggcgataa ttttccatct gccttgcttc
    17821 aggacttcgg cactggctaa ctcaatatta ctgctcattg cggttttaaa agcgccaaaa
    17881 ccataccaca gcaagaaggc tacgccgccc caggtgacca gcgccagcaa ccacggcgac
    17941 tgcatcaata acgcgctgcc accaaaaatc ccggcgcaaa tcaggaccaa atcgctgata
    18001 gcacaaagta aggcaatcat aatgtggtac tgacgacgaa tgccctgatt catcacaaaa
    18061 gcattttgtg gaccgagcgg taggatcata gctgcgccaa gcgcaagacc ttgaaagtaa
    18121 taagaaaaca cgttggatat tccgaattaa gtatctgatg cagagactat atcgcgggat
    18181 tgttattagc ggaaattgat aatattaatt ggcaatcaga aaaactaata agagagaggc
    18241 cagagagtat tatctggcct cagcgttgat tacgcagctt tgtcttcttt cacccgctta
    18301 aagttcacat ccatttgcgg gtacgggaag ctgataccgg cggcatcaaa ttcacgttta
    18361 atacgctcca gcacatccca gtacacgttt tgcagatcgc cgctgttgct ccagacgcgg
    18421 accacgaaat taatcgacga tgcaccaagt tcgttcaggc gcacagtcat ttcgcgatct
    18481 ttcaaaatgc gatcttcaga ctggataata ttggtcagga tctgcttaac ctgatcgata
    18541 tcggaatcat acgccacgcc aataataaat tcgttacggc gaactggctc gcgggagaag
    18601 ttaataatat ttccggcaat aattttaccg ttcggaataa cgataatttt accgtctgca
    18661 gtacgcatgg tggtggagaa aatctgcaca ctcagcacag taccggctac gccgccgagg
    18721 tcaacatatt ctccggcacg gaacgggcgg aacatgacaa gtaacacgcc agcggccagg
    18781 ttagaaagtg acccctgcaa agccagacca acagctaagc ctgcggcacc gagtacagca
    18841 atgactgacg ctgtttgtac acccacgcgt cccagggcag cgattagcgt aaaggcgata
    18901 ataccgtaac ggactaatgc agaaagaaaa tcagcaacag tggcatcgat tttacgggag
    18961 atcatcaggc gattcaccgc gttggaaatc atccgcgcga taatcaaacc aacgatgatg
    19021 atcgcgagtg ccgccacgat gtttactgca taacttagca gcagcgcctg gttagctacc
    19081 agccagcttc ccgcgccgtt tatgctatcg acaacattca aatcttccat tcaatattcc
    19141 ttttcaactg actcaaaatg gagaaataca ctccgccgcc ttatgacggg cagtctgaca
    19201 agggtaaaca ataaagcata attttgccaa atagatcaca gatttctcat ctgaatcacc
    19261 ttgcttttgg cgaaaaaatc cccgcagagc ggggctcaga cagatatcaa tgtgcgcttt
    19321 gtcatgccgg atgcagcgtg aacgccttat ccggcctaca aaaacctgca aattcaatat
    19381 attgcaggat acgtgtaggc ctgataagcg tagcgcatca ggcaattttg cttttggcga
    19441 aaaaaagacc cgcagagcgg gccttgagat aagcagaaag gaatgactta cagaacgtcg
    19501 atcgcgttca gttcctggaa tgctttttcc agacgagcga tcatcgaagt ctgaccggca
    19561 cgcagccata cgcgcggatc gtagtatttc ttgttcggct gatcttcgcc tttcgggtta
    19621 cccagctgac cctgcagata agcttcgttc gctttgtagt agttcagaac gccttcccag
    19681 gttgcccatt gggtatcggt atcgatgttc attttcacta cgccgtagct tacggagtct
    19741 ttgatttcct gagcagtaga accggaacca ccgtggaata cgaagttcag gctgttgtgc
    19801 ggcaggttgt gtttcttaga aacatattcc tgagaatcac gcaggatggt cggagtcaga
    19861 accacgttac ccggcttgta aacaccgtgt acgttaccga aggacgcagc gatggtgaaa
    19921 cgcgggctga ttttgctcag ctcggtgtat gcgtaatcaa cgtcttccgg ctgggtgtac
    19981 agtgcagaag cgtccatgtg gctgttgtcc acgccgtctt cttcaccacc ggtgcaaccc
    20041 agttcgattt ccagagtcat gccgattttg gacatacgct ccaggtattt agagcagatt
    20101 tcgatgttct cttgcagaga ttcttcagac aggtcgatca tgtgagaaga gaacagcggc
    20161 ttaccggtag ctgcgaagtg tttttcaccc gcgtccagca gaccgtcgat ccacggcagc
    20221 agtttcttcg cgcagtggtc agtgtgcagg ataaccggaa caccataatg ttcagccatc
    20281 tggtgaacgt gatgcgcacc agagatcgcg cccaggatag cagcaccctg cggaacgtca
    20341 gatttcacgc ctttaccagc gataaaggaa gcaccaccgt tggagaactg aacgataacc
    20401 ggcgctttaa ctttagcagc agtttccagt acggcgttga tggagtcagt accgacgcag
    20461 tttactgctg gcagtgcgaa gttgttttct tttgctacct ggaaaacttt ctgtacgtca
    20521 tcaccagtga ttacgccagg ttttacgaaa tcaaaaatct tagacatgtc tcttgtcctg
    20581 tatcgtcggg ccttatactc atcatacttc aagttgcatg tgctgcgtct gcgttcgctc
    20641 accccagtca cttacttatg taagctcctg gggattcact ctcttgtcgc cttcctgcaa
    20701 ctcgaattat ttagagtatg aaaaaatgcg tgctctaaaa gcgcgctgaa acaagggcag
    20761 gtttccctgc cctgtgattt tttacttctt agcgcgctct tcgagcatcg ctactgcagg
    20821 cagtacttta ccttccacga attcgaggaa tgcgccgccg ccagtggaga tgtaggagat
    20881 tttgtcagca atgccgaaca ggtcgattgc tgccagagtg tcgccgccgc cagcgatgga
    20941 gaacgcttcg ctgtctgcga tagcgttagc cacgatttca gtacctttgc ggaagttcgg
    21001 gaattcgaac acgccaaccg gaccgttcca cagaatggtt ttcgcattct tcaggatttc
    21061 agccagttcc tgagcggaag catcaccgat atccaggatc tgctcgtcag ctttcacatc
    21121 gttaacagat ttcagggtag ccggtgcagt ttcagagaac tcggttgcta cgcgaacatc
    21181 agacggaacc gggatgttgc aggtggtcag cagacgttta gcttcgtcaa ccaggtcagc
    21241 ttcgtacagg gatttaccca catcgtggcc ttgtgccgcg ataaaggtgt tagcgatacc
    21301 accaccaaca atcagctggt cagcgatttt agacagggag tccagaacgg tcagtttggt
    21361 agatacttta gaaccaccaa cgatagccac catcgggcga gcaggttctt tcagtgcttt
    21421 acccagcgcg tccagttcag ctgccagcag cgggcctgcg cacgcaacgt cagcgaattt
    21481 accgataccg tgagtagaag cctgcgcgcg gtgagcagta ccgaatgcgt ccattacgaa
    21541 cacgtcacac agtgcagcgt attttttgga cagggtttcg tcgtctttct tctcgccttt
    21601 gttgaagcga acgttttcca gaacaaccag ttcaccttca gcaacgtcaa cgccgtcgag
    21661 gtaatcttta accagacgaa ccgggttaga cagtttgtct ttcaggtagt taacaaccgg
    21721 cagcagagag aattcttcgt tgtactcgcc ttcggtagga cgacccaggt gggaagttac
    21781 catcactttt gcgccttgtt tcagggccag ttcgatggtc ggcagagaag cacggatacg
    21841 cgcgtcgctg gttactttcc cgtcttttac tggtacgttc agatccgcac ggataaatac
    21901 acgtttccca gcaagatcca gatcggtcat cttaattaca gacatggtga atcctctcgt
    21961 tgattctaaa agttttgcag acgctgcttg cgtcttacct gaaagcaaca gtagccatag
    22021 ctaacgtcgt gtcgagcatt cggttagcaa agccccattc gttatcgcac cagaccaacg
    22081 ttttgatcag gtgtgcgcca ctgacccggg tttgggtgcc atcgacaatg gcactgtgcg
    22141 gatcgtggtt aaaatctaca gagaccaacg gcaattccgt atagtcaact ataccatgaa
    22201 atgcaccttg tgctgctttt tgcagcaaca ggttgacttc attggctttt acaggtttct
    22261 tcaccgtcac gcttaaatcg attgccgtca catttatggt tggcacacgt accgcaatcg
    22321 cttcaaagcg atcgttaaat tgcggaaaaa atcgtgtgat accggcggcc agtttagtat
    22381 cgaccggaat gatcgactgg ctggctgccc gggtgcgacg caggtcagga tggtatgcat
    22441 caataacctg ttgatcgtgc atggcggagt gaattgtggt cactgtgccg gactcaatac
    22501 cgtacgcatc atctaacaat ttgatgacgg gaattatgca attcgtggta caggaagcgt
    22561 tagaaacgat gcggtgttcc gcacgaagtt gatcctgatt gacgccgtaa acaacggtcg
    22621 cgtcgagatc gttactgcca ggatgtgaaa agagcacttt tttggccccg gcggcaatat
    22681 gcgcttcgcc atgctcgcgg gagccatata cgccggtgca gtcgaggact acatcaacgc
    22741 caagttcacg ccaggggagc gattgcagtg aacgttcatg caatacgcgg atggcgtcat
    22801 caccaacaaa aagttgatcg cgttcctgtc gtacttccca tgcaaaacgg ccatggctgg
    22861 tgtcatattt caacaaatgc gccatgcccg cagcatccgc cagttcgttg attgccacca
    22921 cggtaatttc cgcccggcgt ccggattcat acaaagcacg aaccacatta cgcccgatgc
    22981 gaccgaagcc atttatcgct acgcgtacgg tcatagatct cctgcaaggt tttccctgag
    23041 caaatttgcc agacagagta atccagcaaa tcgtccgggg aaaccttacc tgtcgcaaac
    23101 tgcgactgat tggttaattg tcgaacattt aatcgactga aacgcttcag ctaggataag
    23161 cgaaacgtgg aataaaagga atgattgtcc agtcaaagaa aacaaatatc tgacttacgt
    23221 cacgttttca gccccgcccc tgtggaattt attccacttt gcagaatcac gaataagcgt
    23281 agcaaaatct tttcgttgtg ccgtcacaaa ccctgactta tatcagaata tgactgatgc
    23341 agcgtcgata ttatccgcaa tacgtccgag tttcggagta ctccaatgat gacattgccc
    23401 tacccatatt cttcctccgc acgcttttac gcactgcgcc tgctccccgg gcaggaagtt
    23461 ttttcccagc tacacgcttt tgcacagcag cagcaacttc atgccgcctg gattgcaggc
    23521 tgtaccggta gcctgactga cgtcgccttg cgctatgccg ggcaagaagg caccacgctc
    23581 ctgaacggta cattcgaagt gatctcacta aacggcacgc tggaacagag tggtgagcat
    23641 cttcatctct gcgtttccga tccacacggc acgatgcttg gcggtcatat gatgcccggc
    23701 tgtaccgtgc ggaccacgct tgaactagtg atcggtagcc ttgaggaact ggcgtttagc
    23761 cgccaacctt gcgcgctttc tggttacgac gagttgcata tttcccccgt taaataacta
    23821 ctttcagaga ggttttctta tggcgcgtag ccacttttcc agtcaggcac tggtgttgat
    23881 cgttatatct atcgccatca atatgatcgg cgggcaactg gccagcatgg tcaaattacc
    23941 tatatttctc gactccatcg gcacccttat tagcgcggta ctgcttggtc cggtgatcgg
    24001 gatgctaacc ggcttactga ccaatttact ctggggattg ctgaccgacc cgatcgccgc
    24061 cgcatttgcc cccgtcgcga tggtcatcgg cctggtcgcc ggttgcctgg cgcgagcggg
    24121 ttggtttcgc accctgccaa aagtcgttgt cagcggcgtg attattacgc ttgcggtaac
    24181 ggtcgtcgct gtaccgctgc gtactgcgct gttcggcggt gtcaccggta gcggagccga
    24241 tttgttcgtt gcctggatgc actcgatggg gcaaaacctg gtggagtcgg tggcgattac
    24301 ggtgataggc gcgaacctgg tcgataaaat tcttacagcg gtgattgtct ggcttttact
    24361 gcgccagctg ccgattcgca ccacgcgcca ttttccggca atggctgccg tgcgctaaat
    24421 gcatccattt acctcattaa cactgtgggc attggtggcc tgcaccacgc ttatcctgcc
    24481 cgcgcagacc attttgccga tctatagcgc ggcgaccttt ttctgcctta ttgcgctaaa
    24541 ggccacgcgg cggcgggcaa aatatgtcgt ctggctgatg ttctcgctgg gagccgggct
    24601 gtggctggta cacggtggct ggctaccaga atggctaagc ggcacgccac gctcgccaga
    24661 acggtggtcg catgctatta cgctctggtt gcggattctg gcgattgttt caacctcaca
    24721 gttgtggatg cagtacgtcc ccgtgcaatg ttttattcgc gctctgtttg cttctcgcct
    24781 gccgccaggc gtcgcttatc tgtttgccgg gccgttattg gtcgttgagc agttaaaacg
    24841 gcaattagcg attattcatg aagcgcagcg tgcccgcggc gtgccgctgg atgagggctg
    24901 gtatcagcgt ttacgtgcga tgcccgcgtt aattattccg ctaacacaca atgcgcttaa
    24961 cgatctggct gtccgtggtg cggcgctgga tatgcgggca tttcggatcc acaaccgacg
    25021 aacaacgttg tgggcaccag ccgatagcac cctacaacgc gtagcgcgtt acaccatgat
    25081 cttgctgatg ctgacagaat tcggagcgtg gatatggtta cgttagaaca gttccgttat
    25141 tgcccgacac actcaacgca tcctccgttc tgttatgact ttcactatgt caaaccgggg
    25201 atggtcgcca tctttggtga caacggcagt ggcaaaagta cgctggcaca gctgatggcg
    25261 ggctggtatc cggactttct gccaggagag ataacaggca cggggacgct cctcggtacg
    25321 cccattgggc atctcccgct taatgaacaa tccgccacca tccagctcgt ccagcaatcg
    25381 ccctatttgc agctctccgg ctgtaccttt agcgtggaag aagaagttgc ctttgggcca
    25441 gaaaatctgt gtcttgctga agcagagatt atggcgcgga ttgatgccgc cctgactctg
    25501 accgagtgcc agccgttgcg ccatcgtcac ccggcaacac tctccggtgg agagacacag
    25561 cgcgtggtca tcgcctgcgc catcgcgatg cagccaaagc tcttaattct tgatgaagcc
    25621 tttagccgcc tgactccgca ggccagcgaa atgctgttgc agcgcttaca acactgggcg
    25681 tttgaacgtg gttcgctgat tattcttttt gaacgtcatc gcacaccttt cctgaactac
    25741 tgccagcaag cgtggcaatt gcaaaacgga gcgctccagc cactatgtta acgttaaata
    25801 agatatctta tcgctggcct ggcgcggcaa cggattgtct ttgcgacatt tctctgcaac
    25861 tcaaacaagg tgaatggttg gcgctgaccg gcgacaatgg cgcgggaaaa tcgacgctgt
    25921 tacgagtaat ggcaggtctt cttacgccca cagccggtac ggttatgctg caacaacagg
    25981 cgatgaaaaa cctgaaaaac cgccagcgcg cggcaaaggt tggcgtactg tttcaggaag
    26041 cggaaaacca actttttcac agcaccgtcg ctgacgagat cgcatttggt ttgaaactgc
    26101 aaaaatgccc ggcggatgaa atcactcaac gtacccatgc tgccctgcaa tgttgtcagt
    26161 tagcagacac tgccaactcg cacccgttgg atctgcatag tgcgcagcga cggatggtcg
    26221 ccgtcgcctg cctggaagca ctctctccgc cactattgct gctggacgag ccgagtcggg
    26281 attttgacga aaattggttg agcgtgtttg aaagctggct ggagaagtgc cgccagcgag
    26341 gcacgagcgt cgtagcaatc agtcatgacg ccgcatttac ccggcgtcat ttctctcgtg
    26401 tggtgcgact ggaagatggt ctaatcagga acgttaatcc accagacgat attcacccgt
    26461 agcggtcatc attaaggtca aatttgcagg cagactctct tccagcaccc ggcgaacatt
    26521 cggcccgtcg gtacggtcat aaaaagcttc tgcgtctgcc agagataccc ctcccgccag
    26581 cttgcgccct accagtcgtt cccgcaacgc agtagctggc gcgttgataa agatcgagaa
    26641 atcacaaaat tgcgcaagct ggcaccactt ctcgtcgtcc agcaacaacc agtttccttc
    26701 gacaatgacg agtggtgcgg taacgtgcaa cgcatcttca acaggatcat gcttttgtcg
    26761 atcgtactgc ggccacgtac aatccccttc cacgacctgg cgcagatttt ccgccagttt
    26821 cgcaacgtca aatgtctctg gtgcgccttt gaaggggcgc aattgatgcg catccagcca
    26881 gctattgtaa tggtgaaaac catccatcgg gagcgtctga atagcgggca gttccgggtc
    26941 ttgttgcgcg agatattccc agaaggtggt tagggttgat ttacccgtcc ccggcggagc
    27001 gcaaagaaaa accaccgtac gccgctgcgg atttaccgtc tgtaaggccg ccaacatgtg
    27061 cagcaacggt ttatgcacgc gttctatctc ttcatcatgg tactgcgctt gcacgttcag
    27121 cccattcacc gttaattcaa ttttcacggc gttaattcct ttaaggtggt cgttactgcc
    27181 agcgtcagcg ccgttttgac cattccgcta aaacgcgcct ctgaccagac ggcgaaatcg
    27241 gcgaacttca tcgctttcaa cgcgtcatag agcgtcgcat tgcaggttat gctattgacg
    27301 ttgctgataa aatcccaggt catgtcatcg gcaaatccgg gcgtttcatc ctgttcgttc
    27361 aggtaatgcc agtactggga aaagtgcgtg atatcggcat acagccattt atccattttc
    27421 cccagcgcat aaatcaaacg cagcgcaaca tcaatatcgt caagcgggcc gctttgggcg
    27481 agtaacggct tcactgcata ttccacgatc tcttcatcgt tatcgacaaa cagcgacggc
    27541 agaaactggc gtatcccctg gagcaaaata gaatgcgcag ttttcataaa tgaaaataaa
    27601 ttgtcctgtg catccagttg ctcaagcaca tcatcttctg tcagcgtcgc cattggtctt
    27661 ccgatagatc accatcaatg cagggagtct attatattac acgtttggtc cgcgccgccg
    27721 atcagtaatg tctgcgtccg caccccattc gccgtctgtt ggataccgtt caccaactcc
    27781 ccgcccgtca cacccgtagc gctaaagagg atatcgttcc ccctcaccag ttcatcgagc
    27841 gagtacacac ggttaacgtc aacgcccatt tctctgcaac gcttgcgctc ctgctctgca
    27901 atctgccgat tttccgtgta atccccttta gcctgacaaa agtcaatcag ctcggcctgc
    27961 atatccccac ctaatgcttt aacggcacag gcagaaatca cgccctccgg cgcaccgccg
    28021 atggtgtaca tcacgtcgta aggattatcc tgccagcagg tcagcacgct ggcggcgaca
    28081 tcgccatccg gcagagcgaa aaccttcacg cccagctgtg ttgcttcttc aatcgcggca
    28141 ctcaggcgcg gtttatccag cgtaaccatg cgcagcttgt ccagcggttt acctaacgcc
    28201 cgcgccacgt tacgcaagtt gtccgccaga ggcaaagaca gatcaatcgc cccggcagcc
    28261 agtcgattaa caaccagctt tttcatatac atatcgggcg catgtaacag gctatctcga
    28321 ggggcgaaag ccatgaccgc cagcgcattg ctctgcccca tcgctaccat ccgcgtgcct
    28381 tcaatcgggt caaccgcgat atcgacttct ggcccatcgc ctttgcccac ctcttcgcca
    28441 atccacagca tcggcgcatg gtcaatttct ccttcgccga taaccactcg tccacgaaaa
    28501 gcgacgtcgt ttaatgcctg gcgcatcgcg gtgaccgcca ggccatcaat tttatttttg
    28561 tcgccacatc cggtttgcgg ccaggcagcc aacgctgcct gttccgtaac gcgaaataat
    28621 ggccacgcca gggacatcat tgcgcggcct ccccgatatc aacgccaaat tgtgccagca
    28681 gatatttctc tgcctgttca ttccagatgc cgtgcgtttc ctcaaccagc cgcgccaact
    28741 cttttaacag cggatcggtt ttgccttttt cggcaaaatc ggcaatggca gtgagcggca
    28801 tggtcacgcc gttataaatg agttttttac cgccagggat atccggcaga ttgagcacgg
    28861 tatctggcac cgcatccagc ccaccgatat gcgtgaccat aaaggacggc tgcaactgcc
    28921 ccgtggcgct taacgcaatc gcctctttca tgtcgtccgt tgaaccgccg gatgtaccga
    28981 ctacgtgcgt gctgttgtaa tggacgttgt agaaattaaa cggcactttg aagtttttat
    29041 ccgtcggccc ggcaaagaag ttcagacagc catcttccgc cagtaattcg tcagccatct
    29101 caacgacagc aggcaccgcc gcataaacaa aaatgtcatc gaacccgaca tctcctgtca
    29161 gcgcccgcag cgtctggacc ggatcgctca tccctttggt attcacatac accagctcaa
    29221 tgcctttact ggccgccaga tccaccggca gcagcttctg cacctgcgcc aggcgtttgt
    29281 cgtcgatatc gaccaccacc acccgtgacg gttgtatgcc accgttaatg gcgtaatcga
    29341 tagcgccaat gcccatcgga cccgcacacg ccagcagtgc aatattgccg ccaggtttga
    29401 cgcccatgcg atgctcataa acatattgcg tggtgtgata attggcatga taagcaccaa
    29461 taatgcagca cataggctct gccagcgacg ccgcagcaaa ataagagccg tgatacggta
    29521 atacgcagcc caaattaatg gcgatttcgg gaataatcat ataggtggcg ttaccgccaa
    29581 aatattcgta gctgtagcct gctgaatatc cgcttggtaa ccccatcgcc ggttgcaata
    29641 caaaacgctg gcctttttta tatttgccag tgagattttt acccacttcg acaataaccc
    29701 cggcacattc atgcccggta atgaccggat gattttctaa atcgtcgggt acgcgtttat
    29761 gttcactacc gagtaacgcc gctttccagg tcgataaaca gacgctgtca gaaattacac
    29821 tcactaacag ttcattatcg gtaatttctg gcagttcaaa ttcgcgcaga cggacatccc
    29881 gcttgccata aatagcagca actttggttt tcatttttac ctctgtaatc agtgtttatg
    29941 ttcggcggtt aattgattaa ataaagtgtc gagttttgga tcgccaatat agttattaat
    30001 caatatcagc ggtttatccg tcacacgttt cacgcgccct tccagactgg cgtgagtgac
    30061 gacgatatcg gcatccgcag gcacgttttc tatcgcgtaa tgttttactt ctattgccag
    30121 ccccgctttt tccaggcgtt tacggaaggt ggtcgcgccc atcgcactgg agcccatccc
    30181 cgcatcgcaa acaaaggcga tacgcttaac cctgcttaac gagaatgcac cctcttgttt
    30241 catcgcctta accgcattgg ctgactgagc aaactcatct tcgctctccg tttccaccgt
    30301 tttttccatc ttcagtatca gcgaagtaat agcaaaggac accagggtac ctaccgtaac
    30361 accggcaatt gtcgccagga acgagccttt cggcgtcagt gccaggtaag caaagataga
    30421 ccccggactt gggccagcca ccagaccacc gtccagtaag ttaaacatcc aggtaccaga
    30481 cataccgccc gcaatcatgg caataatagt cagcggcttc atcagcacat atgggaaata
    30541 cagttcgtgg atcccaccga ggaagtgaat aatcatcgcc ccgggcgcag aacgtttact
    30601 catcccttta ccaaacaagg taaacgccag cagcagcccc aggcccggac ctgggttaga
    30661 ggccaccata aagaagatgg atttaccgtt aaccgaagcc tgttgcattc ccagcggata
    30721 atagacgccc tgatcgatcg cattattaag gaaaagcact ttcgccggtt cattgatgac
    30781 tgacagcaga ggcagataac ccgcatgtac cagcgcctcg atacactctt tgacgaaagt
    30841 attggcaatt aacaccgccg ggccgatcac ttcaaaaccc agcagacaaa ggagcatccc
    30901 cgcgatacct aatgagaagt tattgataac catctcaaaa ccggcaggta tgcgtttttc
    30961 cagtgcctta tcgacatatt ttatgaccaa cccaccgagc ggccccatga tcattgagcc
    31021 aaggaacatc gggatctctg cgccaacgat cacaccaata gtacctattc cgcccatgac
    31081 cgccccgcgt ttaccgccga ccagatgacc acctgtagaa ccaatcatca cgggcaataa
    31141 ataggtaatc atcgggccga caattttggc gaaatgttcg ttaggcagcc aaccggtggg
    31201 aataaataac gcagtaataa aaccccaggc aataaaagca ccaatattgg ggatgaccat
    31261 tgcagtcaaa aagcccccaa aagcctggac ctttgcacga gcagacttgt tttccataat
    31321 attatcctgt agaggagaga gtaattatcc gcggctgata atgtccgcta attgtttttc
    31381 tgatgatgct gttaagagtt gttcgagtat ttcttcttcg cacagtaatt cacttaacgc
    31441 ctgaatagcg ccaatgtgcg aatcggcatc ggcagcagag agtccgatga gtaatttaat
    31501 tggttcgtca ttccccggaa aataaacacc ttgttcaagt aatgtcaaag acatcccggt
    31561 tttaagcgcc ccacattccg gtcgcgcatg aggcattgcc acgcctggtg cgagaatata
    31621 ataagggcca ttgttaatgg tggaatcttt aattgcctga atgtaattct cgctgatata
    31681 gtttttatcc agcaatgata ccatcgagaa atcgatagct tcctgccaat cttttgctga
    31741 atgtataact gagattgatg attctggaaa ataatcacta agccgcataa attatcctta
    31801 ttttattttt agggtaccgt ccggcggtaa aaataaaata ctcgtgcggt ttaatgaaag
    31861 caattgagca gggaaaccga tgttttgaaa acagtgaatt attaatttct tatataacat
    31921 tgagttatag atataacaac aaatgtcaat acgcatatcg tgatgcatat tacataattg
    31981 atatttattg catataaata tttgtgatct acaacacgct ttatctattg cttgtccgca
    32041 aacggacata tcaaggtaat aaaaaaggtc gccgaagcga cctttttacc cgaaatgcta
    32101 attacagcag ttcttttgct ttcgcaacaa cgttatcaac agtgaagccg aactcttcaa
    32161 acagcagctc tgccggagca gattcaccga aggtggtcat accgacgata gcaccgttca
    32221 ggccaacata cttgtaccag tagtcagcaa tacccgcttc tacagcaacg cgtgcagtaa
    32281 ccgctttcgg cagtacggat tcacggtaag cagcatcctg cttgtcaaat gcgtcggtag
    32341 acggcatgga caccacgcgc gctttcacgc cttcggcagt cagtttttcg taggcagcaa
    32401 cagccagttc aacttctgaa ccggtagcga tgaaaatcag ttccggctga ccggcgcagt
    32461 ctttcagcac ataaccaccg cgcgcgatgt ttgccagttg ctcttcagtt cgttcctgct
    32521 gcgccaggtt ctgacgggag aggatcagtg cggtcgggcc gtcctgacgc tcaacaccgt
    32581 atttccacgc gaccgcggat tcaacctggt cacacggacg ccatgtagac atgttcgggg
    32641 ttacgcgcag agaagcgacc tgctctaccg gctggtgagt cgggccatct tcgcccagac
    32701 cgatggagtc gtgggtgtaa accatcacct gacgctgttt catcagcgca gccatacgta
    32761 cggcgttacg tgcgtattcc acgaacatca ggaaggtgga ggtgtacggc aggaaaccac
    32821 cgtgcaggga gataccgtta gcaatcgcgg tcataccgaa ctcgcgaaca ccgtagtgga
    32881 tgtagttacc cgcagcatct tcgttgattg ctttagaacc agaccacagg gtcaggttag
    32941 acggtgccag gtcagcagaa ccgccgagga attccggcaa cagcggaccg aaagcttcga
    33001 tagcattctg agacgcttta cggctggcga ttttcgccgg gttagcctgc agtttagcaa
    33061 tgaactcttt tgctttcgcg tcgaagtcag acggcatttc gcctttcata cggcgggtaa
    33121 attcagcggc ttcctgcgga taagctttcg cgtaagcagc gaatttctcg ttccatgcgg
    33181 attctttcgc ctggcctgct tctttcgcat cccactgagc atagatttca gacgggattt
    33241 cgaacggtgc atatttccag cccagctgtt cgcgggtcag ggcaatttca gcgtcgccca
    33301 gcggcgcacc gtgggagtcg tgagtaccgg ctttgttcgg ggaaccgaaa ccgatgatgg
    33361 ttttgcacat cagcagggac ggtttgtcag tcactgcgcg cgcttcttct actgcgcgtt
    33421 tgatggatgc cgcgtcatga ccgtcgatgt cgcgaataac gtgccagccg taagcttcga
    33481 aacgcattgc ggtgtcgtcg gtgaaccagc cttcaacgtg accatcgata gaaataccgt
    33541 tgtcatcgta gaatgcaatc agtttaccca gcttcagcgt acccgccaga gagcaaactt
    33601 cgtgggagat gccttccatc atgcagccgt cgcccatgaa ggcgtaggtg tagtggtcga
    33661 caatgtcgtg gcccggacgg ttaaactgcg ccgccagcgt tttttctgca atcgccatac
    33721 cgactgcgtt ggcaataccc tgacccagcg gaccggtggt ggtttccaca ccagcggtat
    33781 aacctacttc cgggtggccc ggggttttag agtgcagctg acggaagttt ttcagttctt
    33841 ccatcggcag atcgtaaccg gtgaggtgca gcaggctgta gatcagcatg gagccgtggc
    33901 cgttggacag cacgaagcgg tcacggtcag cccaggacgg attctgcggg ttgtgtttca
    33961 ggaaatcacg ccacaggact tcggcaatgt cagccatacc cataggggca cccgggtgac
    34021 cggatttggc tttctgtact gcgtccatgc tcagcgcacg aatagcattg gcaagctctt
    34081 tacgtgagga cattttgact ccagatcgga tgatgaaggg cacgccctta acgacttgac
    34141 gacagcgcgt tttgggctac gccggaaaat ttgccaacaa tttaccgcaa gccgcgcgtc
    34201 atgtacatgg aacatccttt tgccgcttca gaaatctctg gatcatgctc gcatgttgcg
    34261 caatcttctc gcccgtccgc tgcgcttttc cttatactga gactgagcgt cgattcacct
    34321 gcaaacggcg catttttaga ataatcctga ccttgtgcgg aagagaaaac atgaaaattc
    34381 gcgccttatt ggtagcaatg agcgtggcaa cggtactgac tggttgccag aatatggact
    34441 ccaacggact gctctcatca ggagcggaag cttttcaggc ttacagtttg agtgatgcgc
    34501 aggtgaaaac cctgagcgat caggcatgtc aggagatgga cagcaaggcg acgattgcgc
    34561 cagccaatag cgaatacgct aaacgtctga caactattgc caatgcgcta ggcaacaata
    34621 tcaacggtca gccggtgaat tacaaagtgt atatggcgaa ggatgtgaac gcctttgcaa
    34681 tggctaacgg ctgtatccgc gtctatagcg ggctgatgga tatgatgacg gataacgaag
    34741 tcgaagcggt gatcggtcac gagatggggc acgtggcgtt aggccatgtg aagaaaggga
    34801 tgcaggtggc gcttggtaca aatgccgtgc gagtagctgc ggcctctgcg ggcgggattg
    34861 tcggcagttt atctcaatca caacttggtg atctgggcga gaaattagtc aattcgcaat
    34921 tctcccagcg ccaggaagca gaagccgatg attattctta cgatcttctg cgccaacgcg
    34981 gcatcagccc ggcaggtctt gccaccagct ttgaaaaact ggcaaaactg gaagaaggtc
    35041 gccaaagctc aatgtttgac gaccatcctg catccgccga acgcgcccag catattcgcg
    35101 atcgtatgag cgcggatggg gttaagtaag tcagacatac cgatctacaa cataattgta
    35161 ggtcggtaac gcctgaatcc ccgatcagag tagtgatttc actttattta gcaaaacggc
    35221 tacatcatcc ggtcgggtat atcccgtatc agggtctata atcgagctgt aaacatgtgg
    35281 cataatgcgc ggcacccccg cctccagaca agttttcaga atcacactga agttatccaa
    35341 atcaatcccg ccagtcggct caatcagcgt cataccattg cgcgccgccg tggtcgccag
    35401 cgcgtatagc tccggaagcg acctctcgcc gcccatcgga aagaacttcg ccgcgtgtgc
    35461 gcccatatct agcatcattc gcaccgccgt atcgcaggag acgcgggcag gcgtaccctg
    35521 gctgctgcta acgccggtgg aaatcagcac ttcgcccggc gtccccgtcg ggctaaccag
    35581 cgcgttgaca tgggtctgct caccgcccgt agccgcaagc gcgcctgccg cgaaaccaca
    35641 accggtaaag gtctggttaa cgtgtgctgg atgaatctgc gccgcaatca tcgcagcttt
    35701 gtaatactgc gccgggtcac ccgcccccag acctactgaa atggacggta tttccgccat
    35761 ccaacgttta acttcctgga tcccctcatc caccgaggag aactgcgcgg aaagcacgcc
    35821 aatcactgcg tggccttcgg cggcgtcaaa gatttcacgg gcattggcga tatctttcgc
    35881 cagcacatta attgccacgc gattacgata gaaattaatc ttttgcatga tctgcaatct
    35941 ccttcagtcg cgccacgata agtgccattt ccccttccgc caccgtacgc ggatcaaggc
    36001 taaaaacgcc ctggtggagg ttataccgcc gtgcataaat cgcgatatcg ccaccacgca
    36061 gttgcgcttc gacgacgcga gcgtcaactc ccaattcctg cgcatttact cgaatacgaa
    36121 tacgccagat tgcacgcccg gcttcatcct gctcaatatc agccgttaac ccgtgaatcg
    36181 cagaaatcgc ttcaaccact ggctggagct gttccgcggt gatgactgcc tgcccctggt
    36241 gataattctc cagcgcgtaa accagcccaa ccatgttctc cttgccgatt ttcatcgccc
    36301 tggcaatgcc gtgatgctgc gctttgcagg ctgcaatcca gattttttta ccagtgataa
    36361 acccggaggt cggcgcgtta aaggcttttg cgccgctgta gaccaccata tccgctccgc
    36421 tcaccaccca ggcacgtaaa tcctcttcgg cggcggcatc gacaatcagc ggcaggttgt
    36481 acatctgcgc gacatgtgcg aagtcctcga tgttcaacat tcccttctgc acgcagtgat
    36541 gcgacttcac atacagcagc gcggcggtcg cgtcggtaat ggcactctcc agctgccaga
    36601 gagccgccag gttacttgaa cccacctcca caacgcgccc gccacctaag cggatcgcgc
    36661 tgaggatcga tgcaccgtaa tcgacgttat ggccgcgcag catcaccact tcattggcca
    36721 tgccggagct atccggcatg agcgccaccc gggccttgtc gccgcgtgta atggcggcgg
    36781 cgacagcgat ggcgatccct gctgaggcac aggaggtaac gtagctatcc tccgccccgg
    36841 taaagcgaga gaccagctcg ccggttttgt ccaccagctg gtcaatctcc acgaaagagg
    36901 atgccgcgcg cgcggtggcc tgcatcacct ccggcgcgac gctggagacg ccgagaatcg
    36961 tcatcttgcc gcaggcgtta atcacctgtt tcagtcccag ttgttgatag atattctgcg
    37021 tcatggctta cagcactccc agcagcgagc agaccacgct caacgccaca atcgacagca
    37081 gaatcgtggt gtaacgcgga ccctttttca ccaaatagaa gtagatggcg aacactgccg
    37141 ccagcggcag caggccgggc gcgatggagt ccagaatctg ctgtaccacc acttcagagc
    37201 ctttcagtgc gctaattttt aaaggtgagg ttatcttgac aaagctcgcg gatagcgcgc
    37261 ccatcatgat aagccccagt acgttagcgc cgtagataag ctccttgatg cgccctccct
    37321 gcagcagact gataatcgaa tcgcgcccca gcgtatagcc cttgtgcacc atgccgtagc
    37381 tgattgccag cgtaatcgcc gggtagagaa tcagcggcaa aataccgccc atcgcgctgc
    37441 cattggcggc gaacgggata aaaatagcaa tcaacagcgg catcaccgcc gcccagatga
    37501 tggagtcgcc catcccggcc agcggcccca tcaggccggt tttaatcccg gtgatagagg
    37561 catcgttaat cacctcgccg cgggtctttt gctcttccat ggcgatggag atcccctgga
    37621 tcaccgcgcc aaacgtctgc tctgagttga agaagttcag atggcgtttt aacgcctcca
    37681 cctgctcctc cttttgcgga tagagctttt tgataatcgg cgtcatcgag gcgcagaaaa
    37741 tcaggctctg taaacgctca taggagctgg aaacttccgc gcccagccag tagataaacc
    37801 acgctttggt gatatccgct ttggtcagcg tcccggactc ccgcgcgcgc tcaaccagtt
    37861 catgctgcat tacgtcgtcg ctcattatgc cgctccttca tttttcgcca ggcctttaat
    37921 caggaaggcc acgcaggtgc cgaaaatcgc catcgccatg atgtccacct tgaggtacag
    37981 caccgcgaag aatccgccga taaaccacgg cagcaggctc tttttaccaa tcaccataat
    38041 ggtgatggcg aagcccagcg ccgggagaat cccgcccatg atttcaaacg agtgggtcag
    38101 ccagtgcggc atcagcttga ggaaactttc cactacgttc tggccgaagt agttggcggc
    38161 gaacaccact gggaaacgta gtaccagccc cagcagcgcc gggtagagaa aggcgcagcg
    38221 cataatggct gacatattgg ccgtttccgc gtgtttatcg gccatatgca cccaggccgc
    38281 atttagcgtg cgacgcaact ggtcgaggaa tacgccgata acgccaaatg gaatcgccag
    38341 tgcaatcgcc aggttcgggt ccatattggc cttaacggcg ataggaatgg cgatacaggc
    38401 cgccagcgcc gggtctgacg gcacattgcc gcctggcgtt gaggtcacac ccagatagac
    38461 caactgcatc cccgcgccga taatcatcgc cgcaggcata ttgccgagca gcaggccgac
    38521 aaagaccgcc accaccactg gctgtaacag catggcggag aaggtatagc caaggcgcaa
    38581 ccgggcaaac cagtaataca gccccatcag gcttgcaaaa agtaaggtat ccataatttg
    38641 ttatcccatg ttcagagggt cagaattttt tcaggatatc gtccagcgac tgcggtttat
    38701 cttccgggat ggtctggaaa acagccttaa tgccgcgact tttcaaatcg ttaagaatgc
    38761 caacgtcttt ttcatccagc gttatgttct ggaacaccgc tttacggttt ggcccgccgc
    38821 ctaaaccgcc aacctgaata tcagccacgt caaagccctg ctgcaccgct tcctgaatcg
    38881 ccgccagcga cgggaacagc accagcacgt tgccgtcgcc cagctggttc tctttccacg
    38941 cggccgcgaa gctctggttg ctaaagcagt ccactttgat attcggcggc gcggccatca
    39001 ggtagatatt tttcataaac ggatcggcgt ccagctcgtc gctgaccacc gcaatacggt
    39061 tggcctgaga ctgccctacc cacttcgtca ccacctgtcc gtggataaga cggctgtcaa
    39121 tgcgacataa cacgatgttt gccatacgat tttccttact gttgtaattg acgaatatgg
    39181 gagacgacgt ccaggcaact gttgcgccca gcttccacca gatcggccac gcaggcggtc
    39241 agtgggccat gttcgcgctt gtccagcgcc tccagcagca gcgaggcatt aagcccggag
    39301 atcaccgcca ccggataatc ggcgctcagc cgcgccgcca cgttagaggt ggtgccgccg
    39361 ataaaatcgg tcacgataag cgaaccttcc ggcatggttt tcaccacggc ttcgacgcgc
    39421 tggtaaaact cgcccagcgt atcgacgggc atcagcgcga tttccgtcac gccttcgatt
    39481 tcgcccatca ccatgcgcag actgttgcag agctgcagcc cccagccgcc gtgagttagc
    39541 agcagaattt caggtaacgg atgaataggt acagttgtgg tcaaaatagc ctcctttgct
    39601 ccagtgttta catttcatca ggagcaaagg acgtgccaga atttgtgtca cgcacagagg
    39661 aaatgagatt ggatggggcg ttaagccccg taaaccaggg gatcagctgt agagcagttc
    39721 gtagatataa aaatattctg cgtccgataa acggatagcg taagcctctt caatcggcaa
    39781 aaaggaggat ttgatgacgc taaaggcacg agcgtccaga tcaagccggt tttctagcgc
    39841 catctgtaac ggtttgcggt taatcacgat acgctcgacc atgcagcagc agtggatcag
    39901 aaaacgtagt gtcacctggc ggctcggttt cagggagagc gtgttggtta agtagttcag
    39961 aacatcctcg atctctttca gaattcgctg cgggttgagc accgaaatat ggttgatgat
    40021 gctctccatg gtcagcgcgc tgataaagcg catggcgctg cgctccatct ccagtcggcg
    40081 ctcgctgctt gagagatccg gcgtcagcag gctgagcacc agctccggcc cttgctcgga
    40141 aaacagttcc tccagcgaga taaacggtat atccggcagt cccggctgga aggtgccgac
    40201 gatccctgcc agccgttcac tggcattaag cgcctgctga atacgctcca ggctgcgcac
    40261 ctcgttatag tcgagaatca ccatacgcgt gtcctgcgac atcagctcgc caaagctctc
    40321 ctccagcacc tttttgatct tttccgccgt ccccattccg gttatacatg agataaccag
    40381 cactttgcca ccgctctctt gctgcggcgt gcagagccga caagggatat ttttactctg
    40441 cataagcaca gccaattgcg atagatcgcc agtttcgtag cttaaatcca gcccaacctc
    40501 cagcaggctg gtcagagtga tatttggcaa caacagaacg tcaatctgga acagcttgct
    40561 gatggtgctg ccaaaatgga ccagcgatcc gatatccacc atcagtatca gccgctgcca
    40621 gcaccggtcc tgaatcatcc gggtcagggt ttccagggtg tcatgcaccg actgctcaaa
    40681 tggcatatca attgcgtaga acagatcgcg ctccagcacc cggttcacat attgcgcctg
    40741 gctcgttgcg gtggtcgcac cgtgagcgat tagaatcacg ccgcaatccg ggctggtgtc
    40801 gatgcgctgg cggtagtggc gacactcttt caggaacagg cacagccaga ccacctccgt
    40861 cgccgggcac tgaatatgca acaactcatt gattttgcgg cacagaagcg tggcattgtc
    40921 atactcatct ttacagcgat ccagaatcag gctggatgaa tagagttgag gtataagccc
    40981 tcgccgcaca tagccaatca gcgcaagaaa gtgtttacgt aacgggttcg ccaggttttc
    41041 tggcaatgta aatcctaata cctgctccac gtaaccgatc agcagcgtca cacgctcctc
    41101 aatttgatcg ccatagcgcg gtggatgcgc cgcactatgg cggctataaa gaccatattc
    41161 aaaaatcgaa ctgagtttgt ttttcaggat agccagcgtt tctgcgggcg gcacgttact
    41221 gttgcgcaga ttgacatatt cacgagtcag gaaactgtaa aacaggtcgc tctcttctat
    41281 ttccgcgctg gtcgccaacg aattttttaa cgcgggcaac gtacgcgcat ccacgttcag
    41341 acgatcttta ccgccaaaca acgcatcgac cagcagacgc tgctctggcg tggtattaaa
    41401 cgacatctcc gccagtcgtt tatctaactc tagcgtatca ttacgctcgg tcattcccga
    41461 cgcccacgcc tgcgcgcaca agaactgaat atcactttta agctggccga tattgccttc
    41521 caggggttta cttaacaacc aatgcaagag cgttttatca aggcttatgg tacgttcaat
    41581 tttacgactt tcacgctgaa agaagcttac aatcaattcg agctgctcct cgaccgaacg
    41641 ctggcgaata cccggtaagt caatacatac ctggatacgc cgctggaagg tacgcaatag
    41701 tgccgagttg accgactctg tagtggcgca aataaggcgc accgaaattg aatatgttgg
    41761 cccacttgaa ccaagtgggc ggtattcacc tttatcgaga atagaaaaga gcttctcttg
    41821 tccttcgtac gggaggcgat gcacttcatc cagaagtaaa taacctccgt cagcttgctc
    41881 caccaatccc gttttattct cacttgcccc ggtaaatgcc cccttatggt gaccaaacag
    41941 atgtgaggaa agcaactccg gattatgagc atattcggca cagttaaagt aaaccaacgg
    42001 aggtggcgaa cctatggtgt gcttgcaagc gaagcaatgc attaactcag caaagaatgt
    42061 tttgccaaca ccagatgcac cggttaacag cacatgtagt ccgtggggat aaagcaccgc
    42121 tgcgcggcct ttttctaccg catcacggag actacggtcg tatccgataa gtccggaaaa
    42181 cggatcatca tctgtatatt tttctttctt tggcaataaa ctagccactg aatgaactat
    42241 gcattcgcaa tcatccagtt ttcgattaat gagcaattct agtgcgtgac gatgtagaaa
    42301 aaaaacagga cgtccctgac tttttaccac caggccttca ttccataatt gattaagatc
    42361 tttactaacc gagttgcgtg ccagcccaag gttaaaacca atcgcctcag cagtaaatgc
    42421 tttctccttc gctaaatcag acagttccag cccatacgtc agccgctcca gctccttgtg
    42481 aataacatca atccgtctca tttctttacc gcttaaaaaa caatagttgt tcagaacata
    42541 cactatttgt aaacgtaaga atgagaacta tcgggaagaa ttaatgttcc ttgtggcaat
    42601 ctggtttctg aatattcgtg ttttaaatat tacttatggt ctgaaacaac caggcaggtg
    42661 aacagagcta agaaaaagta gcgtagaaat ataaaaacat atatcgcact gatgaatgtt
    42721 tcccggcatg actatgcaca ccgggaaata tttagtaaat tactcgccct ttttcgccgc
    42781 ctgaatatac agcatctcca gcgccagcgt cgccgcagcc agagcagtga tttccgactg
    42841 atcgtatgcc ggagccactt ccactacgtc catcccaacg atgttgagat ctttcaggcc
    42901 gcgtaccagt ttaatggcgc gatcggaggt cagaccgcca attaccggcg taccggttcc
    42961 cggcgcaaat gcaggatcca ggcagtcgat atcaaaggtc aggtaaaccg gcatatcacc
    43021 cacaatttgt ttcacctggg cgataacgtc atccacgctg cgatcgttca cctggcaggc
    43081 gtccagcacg gtaaagccgt tgtctttatc aaattcggta cgaataccaa tctgcacgga
    43141 atgattcgga tcgatcagac cttctttcgg cgcggtatag aacatggtgc cgtggtcaaa
    43201 ttcacaaccg ttcgcgtagg tatcggtgtg ggcgtcaaag tgtaccagcg ccattttgcc
    43261 gaaatgcttc gcatgagcac gcagcagcgg cagcgtaaca aagtggtcac caccgaagga
    43321 gagcatacgc ttaccggcag ccagcagctt ctcggcgtgc gcctgcagct tctcgctcat
    43381 ctcacgggca tcgccgaaag catataccag atcaccgcag tccacgacgt tcagacgctc
    43441 acgcatgtcg aaattccacg ggaagcggtt gtgttcccag gccagattcg tcgaaacctg
    43501 acggatagct gccggaccgt ggcgtccccc cgcacgacca gaagtggcca tatcgaacgg
    43561 cacaccagta atcacccagt cagcatcgct gtcatacggc tggaagttca tcggcaggcg
    43621 taaaaaacca aaggcattgg aaaccagtga gttatcgtat tgatgaccta aggtgctcat
    43681 ggatggactc ctattacaaa gtcgatataa aaaaaccctt ccgcgtcgtt aggcccgacg
    43741 aggaagggtt ggatttgcga aacacaagtt tgtgctaatt atcgccttaa atgggcgagg
    43801 catcaagcga ggttgctatt tccgacacgg tggcggcgca tagccattgt tatctgctgg
    43861 aaaagcttgt ccagagcacg gtggcagcgc gttttgcgcc gggccttgta attcaaaggc
    43921 ggcaaagccg attaccccgg tattacgcac atcgccttgc gccgaattgg ctgcgtagga
    43981 atcaacaacg ttggcgaact gaaaagccgc ttcggtatgt ttatctttgc ggaatccttt
    44041 aatcgctaac gaatctccgg cattcacaat atagccgttg ttattcagcg aaccttgctt
    44101 gccgttcagc acatccaggc catcaaccgt agcaacgatc tcgtagttgg tgttacgact
    44161 gtaattacga acatataatt gataattcat tccgactctg gcgtggacaa tatagcgtgg
    44221 ttcattattg gatgaatcat aaacacgcgt aataggtaga gaattaaaat taccatcgcg
    44281 aatagcatat tccagttcgt taatacgaat gctataaacg cggtcgtagc cggaaggaac
    44341 attagtcgaa taactaataa cgatcatctc cgccggatcg cgccaggctc gctcagcaga
    44401 tacgccctga accgttgagt gcacatcccc accccaggcg gtgccgacat tacccgatgc
    44461 actgctatta tactgcgcat tactggaaca accggccagc agcacgatta atgccaccaa
    44521 cgcgctacgt actttccatt ttttcataac cgttccctct gcatttaatt aatgcagcga
    44581 ttgtaggg
//
LOCUS       DAPHDW010000023        44545 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.023, whole genome shotgun sequence.
ACCESSION   DAPHDW010000023 DAPHDW010000000
VERSION     DAPHDW010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 44545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.023"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(220..1584)
                     /gene="mnmE"
                     /locus_tag="RH057_001216"
     CDS             complement(220..1584)
                     /gene="mnmE"
                     /locus_tag="RH057_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HDV3127621.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVIAELDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
     gene            complement(1690..3336)
                     /gene="yidC"
                     /locus_tag="RH057_001217"
     CDS             complement(1690..3336)
                     /gene="yidC"
                     /locus_tag="RH057_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HDV3127622.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISTFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIAAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            complement(3339..3596)
                     /gene="yidD"
                     /locus_tag="RH057_001218"
     CDS             complement(3339..3596)
                     /gene="yidD"
                     /locus_tag="RH057_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HDV3127623.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            complement(3560..3919)
                     /gene="rnpA"
                     /locus_tag="RH057_001219"
     CDS             complement(3560..3919)
                     /gene="rnpA"
                     /locus_tag="RH057_001219"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HDV3127624.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            complement(3936..4076)
                     /gene="rpmH"
                     /locus_tag="RH057_001220"
     CDS             complement(3936..4076)
                     /gene="rpmH"
                     /locus_tag="RH057_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HDV3127625.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            complement(4306..4386)
                     /locus_tag="RH057_001221"
     CDS             complement(4306..4386)
                     /locus_tag="RH057_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127626.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            4683..6086
                     /gene="dnaA"
                     /locus_tag="RH057_001222"
     CDS             4683..6086
                     /gene="dnaA"
                     /locus_tag="RH057_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HDV3127627.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAARQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            6091..7191
                     /gene="dnaN"
                     /locus_tag="RH057_001223"
     CDS             6091..7191
                     /gene="dnaN"
                     /locus_tag="RH057_001223"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HDV3127628.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQQHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            7191..8264
                     /gene="recF"
                     /locus_tag="RH057_001224"
     CDS             7191..8264
                     /gene="recF"
                     /locus_tag="RH057_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HDV3127629.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMADTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            8293..10707
                     /gene="gyrB"
                     /locus_tag="RH057_001225"
     CDS             8293..10707
                     /gene="gyrB"
                     /locus_tag="RH057_001225"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HDV3127630.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            10947..11345
                     /locus_tag="RH057_001226"
     CDS             10947..11345
                     /locus_tag="RH057_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418153.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HDV3127631.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQGNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSPQANNDLLSAGMELLKGKLFR"
     gene            11460..12272
                     /gene="yidA"
                     /locus_tag="RH057_001227"
     CDS             11460..12272
                     /gene="yidA"
                     /locus_tag="RH057_001227"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HDV3127632.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMELPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(12318..12974)
                     /locus_tag="RH057_001228"
     CDS             complement(12318..12974)
                     /locus_tag="RH057_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127633.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            13252..13941
                     /gene="dgoR"
                     /locus_tag="RH057_001229"
     CDS             13252..13941
                     /gene="dgoR"
                     /locus_tag="RH057_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HDV3127634.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLPAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            13938..14816
                     /gene="dgoK"
                     /locus_tag="RH057_001230"
     CDS             13938..14816
                     /gene="dgoK"
                     /locus_tag="RH057_001230"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HDV3127635.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARTLAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVTAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            14800..15417
                     /gene="dgoA"
                     /locus_tag="RH057_001231"
     CDS             14800..15417
                     /gene="dgoA"
                     /locus_tag="RH057_001231"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HDV3127636.1"
                     /translation="MQWQTKLPLIAILRGIKPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPSDIAVFAVGGVTPE
                     NLAQWIDAGCAGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            15414..16562
                     /gene="dgoD"
                     /locus_tag="RH057_001232"
     CDS             15414..16562
                     /gene="dgoD"
                     /locus_tag="RH057_001232"
                     /EC_number="4.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
                     /protein_id="HDV3127637.1"
                     /translation="MKITKITTYRLPPRWMFLKIETDEGVVGWGEPVIEGRARTVEAA
                     VHELGDYLIGQDPSRINDLWQVMYRAGFYRGGPILMSAIAGIDQALWDIKGKVLNAPV
                     WQLMGGLVRDKIKAYSWVGGDRPADVIDGIKTLREIGFDTFKLNGCEELGLIDNSRAV
                     DAAVNTVAQIREAFGNQIEFGLDFHGRVSAPMAKVLIKELEPYRPLFIEEPVLAEQAE
                     YYPKLAAQTHIPLAAGERMFSRFDFKRVLEAGGISILQPDLSHAGGITECYKIAGMAE
                     AYDVTLAPHCPLGPIALAACLHIDFVSYNAVLQEQSMGIHYNKGAELLDFVKNKEDFS
                     MVGGFFKPLTKPGLGVEIDEAKVIEFSKNAPDWRNPLWRHEDNSVAEW"
     gene            16637..17974
                     /locus_tag="RH057_001233"
     CDS             16637..17974
                     /locus_tag="RH057_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3127638.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASTLWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(17971..19035)
                     /gene="cbrA"
                     /locus_tag="RH057_001234"
     CDS             complement(17971..19035)
                     /gene="cbrA"
                     /locus_tag="RH057_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418145.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HDV3127639.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPTSVEVYHDSLCRNIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNSITNCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSADILRAVLLKQPEKLNTAYWRATRKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGMAHIPQLKD"
     gene            19136..20350
                     /locus_tag="RH057_001235"
     CDS             19136..20350
                     /locus_tag="RH057_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HDV3127640.1"
                     /translation="MKQITFAPRNHLLTNTNTWTPDSQWLVFDVRPSGASFTGETIER
                     VNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHRRGVIAE
                     GGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERVSFTYNDHVMHELDPALDLRNV
                     GVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVGNHALAF
                     IGDTLSPKGEKVPELFIVELPQDEAGWKAAGDAPLSGTETTLPAPPRGVVQRRLTFTH
                     HRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRQLTHNKTDIQ
                     SAFNWHPSGEWLGFVLDNRIACAHAQSGEVEYLTENHANPPSADAVVFSPDGQWLAWM
                     EGGQLWITETDR"
     gene            complement(20352..20684)
                     /locus_tag="RH057_001236"
     CDS             complement(20352..20684)
                     /locus_tag="RH057_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV3127641.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     misc_feature    20924..20996
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            20990..21403
                     /gene="ibpA"
                     /locus_tag="RH057_001237"
     CDS             20990..21403
                     /gene="ibpA"
                     /locus_tag="RH057_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HDV3127642.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     gene            21515..21943
                     /gene="ibpB"
                     /locus_tag="RH057_001238"
     CDS             21515..21943
                     /gene="ibpB"
                     /locus_tag="RH057_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HDV3127643.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            22140..23801
                     /locus_tag="RH057_001239"
     CDS             22140..23801
                     /locus_tag="RH057_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HDV3127644.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(23798..24514)
                     /locus_tag="RH057_001240"
     CDS             complement(23798..24514)
                     /locus_tag="RH057_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3127645.1"
                     /translation="MIYKSIAERLRIRLNYADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IGQECGILIGGNYESLTPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEYQVEYHLRRLHPEKS"
     gene            24810..26426
                     /locus_tag="RH057_001241"
     CDS             24810..26426
                     /locus_tag="RH057_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV3127646.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGLAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLHEQLDSLINSHQSAENVAITEAV"
     gene            26426..27240
                     /locus_tag="RH057_001242"
                     /pseudo
     CDS             26426..27240
                     /locus_tag="RH057_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(27237..28130)
                     /locus_tag="RH057_001243"
     CDS             complement(27237..28130)
                     /locus_tag="RH057_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV3127647.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHGNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDIPVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMYADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            28291..30006
                     /locus_tag="RH057_001244"
     CDS             28291..30006
                     /locus_tag="RH057_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HDV3127648.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAMGKGSFM
                     QGIEQLTTVHAEKLNSIGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPMVGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWRNVKIAS
                     IGILFAMIGVYAGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWRHRHDPAVTFTPD
                     AKDSL"
     gene            30003..31496
                     /locus_tag="RH057_001245"
     CDS             30003..31496
                     /locus_tag="RH057_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV3127649.1"
                     /translation="MKRPNFLFIMTDTQATNMVGCYSGKPLNTQNIDSLAAEGIRFNS
                     AYTCSPVCTPARAGLFTGIYANQSGPWTNNVAPGKNISTMGRYFKDAGYHTCYIGKWH
                     LDGHDYFGTGECPPEWDADYWFDGANYLSELTEKEISLWRNGLNSVEDLQANHIDETF
                     TWAHRISNRAVDFLQQPARAEEPFLMVVSYDEPHHPFTCPVEYLEKYADFYYDLGEKA
                     QDDLANKPEHHRLWAQAMPSPVGDDGLYHHPLYFACNDFVDDQIGRVINALTPEQREN
                     TWVIYTSDHGEMMGAHKLISKGAAMYDDITRIPLIIRSPQGERRQVDTPVSHIDLLPT
                     MMALADIEKPEILPGENILAVKEPRGVMVEFNRYEIEHDSFGGFIPVRCWVTDDFKLV
                     LNLFTSDELYDRRNDPNEMHNLIDDIRFADVRSKMHDALLDYMDKIRDPFRSYQWSLR
                     PWRKDALPRWMGAFRPRPQDGYSPVVRDYDTGLPTQGVKVEEKKQKF"
     gene            complement(31543..31992)
                     /locus_tag="RH057_001246"
     CDS             complement(31543..31992)
                     /locus_tag="RH057_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127650.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTFESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDVDVDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGGGTLAFHTLLFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            32102..32449
                     /locus_tag="RH057_001247"
     CDS             32102..32449
                     /locus_tag="RH057_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HDV3127651.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            32439..32801
                     /locus_tag="RH057_001248"
     CDS             32439..32801
                     /locus_tag="RH057_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HDV3127652.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            32798..33295
                     /locus_tag="RH057_001249"
     CDS             32798..33295
                     /locus_tag="RH057_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV3127653.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(33303..34487)
                     /gene="emrD"
                     /locus_tag="RH057_001250"
     CDS             complement(33303..34487)
                     /gene="emrD"
                     /locus_tag="RH057_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HDV3127654.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(34906..34995)
                     /gene="tisB"
                     /locus_tag="RH057_001251"
     CDS             complement(34906..34995)
                     /gene="tisB"
                     /locus_tag="RH057_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HDV3127655.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            35559..35657
                     /gene="ivbL"
                     /locus_tag="RH057_001252"
     CDS             35559..35657
                     /gene="ivbL"
                     /locus_tag="RH057_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HDV3127656.1"
                     /translation="MTTSMFNAKLLPTALSAAVVVVRVVVVVGNAP"
     gene            35763..37451
                     /gene="ilvB"
                     /locus_tag="RH057_001253"
     CDS             35763..37451
                     /gene="ilvB"
                     /locus_tag="RH057_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HDV3127657.1"
                     /translation="MASSGTTLTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPGPVWIDIPKDVQTAVFEIETQPAMAEKAAAPAFSEESIRDAAAMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPQVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            37455..37745
                     /gene="ilvN"
                     /locus_tag="RH057_001254"
     CDS             37455..37745
                     /gene="ilvN"
                     /locus_tag="RH057_001254"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HDV3127658.1"
                     /translation="MQNTTHDNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIDKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            37820..38410
                     /gene="uhpA"
                     /locus_tag="RH057_001255"
     CDS             37820..38410
                     /gene="uhpA"
                     /locus_tag="RH057_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HDV3127659.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            38410..39912
                     /gene="uhpB"
                     /locus_tag="RH057_001256"
     CDS             38410..39912
                     /gene="uhpB"
                     /locus_tag="RH057_001256"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418124.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HDV3127660.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHPVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLSLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            39922..41241
                     /gene="uhpC"
                     /locus_tag="RH057_001257"
     CDS             39922..41241
                     /gene="uhpC"
                     /locus_tag="RH057_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HDV3127661.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIINILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAAAALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQAPREA"
     gene            41379..42770
                     /gene="uhpT"
                     /locus_tag="RH057_001258"
     CDS             41379..42770
                     /gene="uhpT"
                     /locus_tag="RH057_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HDV3127662.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(42815..>44545)
                     /gene="adeD"
                     /locus_tag="RH057_001259"
     CDS             complement(42815..>44545)
                     /gene="adeD"
                     /locus_tag="RH057_001259"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HDV3127663.1"
                     /translation="SRAEYQELLAVSRGDAVADYIIDNVSILDLINGGEISGPIVIKG
                     RYIAGVGAEYADAPALQRIDARGATAVPGFIDAHLHIESSMMTPVTFETATLPRGLTT
                     VICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCDVNGASFTLEQML
                     AWRDHPQVTGLAEMMDYPGVISGQNALLDKLDAFRHLTLDGHCPGLGGKELNAYITAG
                     IENCHESYQLEEGRRKLQLGMSLMIREGSAARNLNALAPLINEFNSPQCMLCTDDRNP
                     WEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGLLAPGKQADIVLL
                     SDARKVTVQQVLVKGEPIDAQTLQAEESARLAQSAPPYGNTIARQPVSASDFALQFTP
                     GKRYRVIDVIHNELITHSHSSVYSENGFDRDDVSFIAVLERYGQRLAPACGLLGGFGL
                     NEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNGQVQSHLPLPIAG
                     LMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPALKLTSQGLFDGEK
                     FAFTTLEVTE"
BASE COUNT        10256 a        11454 c        12092 g        10743 t
ORIGIN      
        1 cgaggctaca gtataatact caagaatttt gtctattcta aaattgttta aatgtgaatc
       61 gaatcacaat cgttcgggga gcaatattaa atgctttttt ttaagattat aaattacgta
      121 agaattttaa tgtttaaaaa aagggttgag gaataacagg agtgctaaca atgaagcgaa
      181 gccgcatctg acagtcagaa tacggcttcg taagcgcggt tacttaccaa tacagaagct
      241 ggagaaaatc cgccccagca ggtcatcaga agtaaattcc ccggtgattt cgcttaagtt
      301 ctgctgtgcc aggcgcaact cttccgccag cagttcgccc gcccaggcac ctaacagttg
      361 cgctttacct tgctgcaaat gctctgccgc ctgttccagc gcctgtaggt ggcgacggcg
      421 cgccaggaag ccgccttcca tgttggtgtc aaagcccatg ctctgtttga gatggttacg
      481 cagcacatcc acaccttcac cagtccttgc cgagagacga attaacgcgt gaccgttcac
      541 ttcactcatt cccagcgttt cgccggtgat atcggcttta ttgcgcacca cggtgatcgg
      601 cagtttcgct ggcagacggg cgataaattc cggccagatc tctgccggat caacggcgtc
      661 tgtcgtggtg ccatcgacca taaacagcac gcggtcggcc tgttcaattt cctgccacgc
      721 gcgctcgata ccaatacgtt ccacttcgtc actggcttca cgtagcccag cggtatcgat
      781 gatatgcagc ggcattccgt caatgtggat atgctcacgc agcacgtcac gtgtggttcc
      841 ggcgatatca gttacgattg ctgcttcacg gcccgccagc gcgtttaaca ggctcgattt
      901 accggcgtta ggacgtccgg caatcaccac cttcatccct tcgcgcaaca aactaccctg
      961 acgtgcttca gcacgcactg catcgagttc ggcaatcacg tcattaagct gggcttcaat
     1021 tttgccgtcg gagaggaaat cgatctcttc atcagggaaa tcaattgccg cttcgacgta
     1081 aatgcgcaag tgggtgaggg cttccaccag atggttgacc cgtgcggaga atgcgccttg
     1141 cagcgagtta agtgccgaac gggccgcctg ttccgaactg gcgtcgataa gatcggcaat
     1201 cgcctcggcc tgggctaagt cgagtttatc gttaagaaac gcgcgttcgg aaaactcacc
     1261 agggcgagca atccgcaggc cgggaatggt cagaatgcgt tttaacagca ggtcgaggat
     1321 caccggaccg ccatgacctt gcagttccag cacatcttcg ccggtgaacg agttcgggcc
     1381 agggaaccat agcgcaatcc cctgatcgag cacgctgccg tcggcgtctt taaacggaag
     1441 ataatcggcg tagcgcggct taggtagttt acccagcacg gtttcggcaa cttcacgggc
     1501 tttgaagccg gagatgcgca ggatgccaac gccgccacgt cccggaggcg tggcctgggc
     1561 tacgatagtg tcattatcgc tcatgatgtt cctgttgctt tgtgtggcgg atgcgcggtg
     1621 cttatccgcc ctacgaaaag aaaaaaggcg gtcaactgac cgcccttatt ttagcgaaaa
     1681 ctcaccgaat caggattttt tcttctcgcg gctatgcagg ccacgttttt ccagaccacg
     1741 gtaaatcagc tgctgctgaa taatggttac caggttgctg acgatatagt acagcaccag
     1801 acctgacggg aaccacagga agaacacggt gaagatgacc ggcataaagg tcatgatctt
     1861 ctgctgcatc gggtcggtca ctgtggtcgg cgacatcttc tgaatgaaga acatcgttac
     1921 gcccatcagg atcggcaaga tgtagtacgg gtcctgtgcc gacaggtcgt ggatccacag
     1981 tgcaaacggt gcctgacgca gttcaacgga acccatcagc atgtagtaca acgccaggaa
     2041 gattggcatc tggatcagca gcgggaagca gccgcccagc gggttaacct tctcagcttt
     2101 gtacagcgcc atcatttcct ggctgatacg ctgtttgtca tcgcccagac gctcacgcat
     2161 tgcctgaatc ttcggctgca gcatacgcat cttcgccatg gaggtgtact gcgctttggt
     2221 cagcgggtac atgatgccac gaacgataaa ggtgatgatg ataatggaga agccccagtt
     2281 acccacaaag ctatggatcc atttcagcag tttgaacagc ggctgagaga tgaaccacaa
     2341 ccaaccgtaa tcaacggtca gatccaggtg cggagcaaca gctgccattt tgtcctggat
     2401 ttccgggcca acccacaggg tgctgttcat cgcgccagtc tgaccaggct gaaccagtac
     2461 cggctgagat ttatagccga tagcggcgat gccgttaccc agattagcgg tatagaagtt
     2521 gttggtaccg tcgttatgcg ggatccacgc cgtcgcgaaa tactgttgca gcatcgccac
     2581 ccaaccacct ttcgaagaga tgttcaggtt ttcgttatcg gcaatggtat cgaacttgta
     2641 tttctcgtac ttctcgtcag gcgtggagta cgccgcgccg cggaaggtgt gcagtgcgaa
     2701 gttgctgctt ccggtatcga gatgcggtgg cagagtgatg gattgcttca actgaccaaa
     2761 ggtggagatt tccagcggtt tctcgccagc gttctgcacg ttgtagttga cgttgacagc
     2821 gtaatcacca cgtttcagga caaacgtttt ggtaaacgtg ttgcctgccg cgtcggtata
     2881 cgtcatcggc acctgcagtt cgttttgacc ttcagccagc acataagcgt ctttttcaac
     2941 gttatacagc ggacgcgggc cgttagccgg gttatccggg ccatcacgac cggtcagacc
     3001 gctctgtgcc tgataaataa actgcggtga agtttccagc agctggaacg gctgggtaga
     3061 gttcagctct ttcgggtaag caggcagcag agcttgctca acatcaccac cacgggtgtt
     3121 gatggtcaga tcaagcacgt cggtcttaac cgagatcagt ttcccctggc cactggccgg
     3181 tacgccctgg tcggcggcgc tacccgctgc ggtggtcgtt gtctgcgtgg tctgttgggc
     3241 ctgaggttgc gggtttttat cctgctccca ggcttgccag atcatgaaag acacgaacag
     3301 caaagcgatg actaaaagat tgcgttgcga atccatcgtt agtgttctct ggtatcaaat
     3361 ggtccgggcg ggacgggatc gtcaccacca gggtgtaaag ggtggcattt taatacgcgt
     3421 ttcaccgtca accaactgcc ttttatcact ccaaacctgc gcaatgcctc aattccgtag
     3481 cttgaacagg ttggagtgaa acgacaatgc ggcccgagta gcggactaat caggcgttga
     3541 tagacccgaa tgagggctat caggacccgc gagccaggcg acagtggcgg cgccataatt
     3601 tttccaacgc ttccgagaga gcacggttat cgaggtcggc aacccctttt ttcgccacca
     3661 ccacgaaatc catagccggg agttcatgtt ggcgcagacg gaagctttca cgcgtcagac
     3721 gtttaatccg attgcgttca tgggcgcgtc gaacgttttt cttggcgact gtaagaccga
     3781 tacggggatg ccccagcgaa ttcaggcggc cgagaatggt aatttgcggc gtgccagccc
     3841 gttgtggctg ctggaagacg aatgtgaatt gactgggagt taacaagcgt aactccctgg
     3901 gaaatgcgag cttaaccact caggggttag ctttattact tagaaacggt cagacgagcg
     3961 cggcctttag cacgacgacg tgccagaacc tgacgaccat ttttagtagc catacgagca
     4021 cggaagccgt gagaacggtt gcgcttcagt acagacggtt gaaaagtgcg tttcatggcg
     4081 atttctacct aaacttgaat aaattcaatg gctttattgg atatccgccg aaaaatgaaa
     4141 cgatggacac cgaagccatg ggtgattaaa gaggccggat tgtaataatt gtacactccg
     4201 gagtcaattc tctttcctta tttaccgcgc ttttccgcac cttttcgcag ggaaaatgta
     4261 cgacctcaca ccagtggaaa ccagcatggc gcgccgggtg gaggattata cgggctgatg
     4321 ggtaaagcgc aaggatcgtc ctggatcttt attagatcga ttaagccaat ttttgtctat
     4381 ggtcattaaa ttttccaata tgcggcgtaa atcgtgcccg cctcgcggca ggatcgttta
     4441 cacttagcga gttctggaaa gtcctgtgga taaatcggga aaatctgtga gaaacagaag
     4501 atctcttgcg cagtttaggc tatgatccgc ggtcccgatc gttttgcagg atcttgatcg
     4561 ggcatataac cgcagacagc ggttcgtgcg tcaccctcaa gcagggtctt ttcgacgtac
     4621 gtcaacaatc atgaatgttt cagccttagt cattatcgac ttttgttcga gtggagtccg
     4681 ccgtgtcact ttcgctttgg cagcagtgtc ttgcccgatt gcaggatgag ttaccagcca
     4741 cagaattcag tatgtggata cgcccattgc aggcggaact gagcgataac acgctggccc
     4801 tgtacgcgcc aaaccgtttt gtcctcgatt gggtacggga caagtacctt aataatatca
     4861 atggactgct aaccagtttc tgcggagcgg atgccccaca gctgcgtttt gaagtcggca
     4921 ccaaaccggt gacgcaaacg ccacaagcgg cagtgacgag caacgtcgcg gcccctgcac
     4981 aggtggcgca aacgcagccg caacgtgctg cgccttctac gcgctcaggt tgggataacg
     5041 tcccggcccc ggcagaaccg acctatcgtt ctaacgtaaa cgtcaaacac acgtttgata
     5101 acttcgttga aggtaaatct aaccaactgg cgcgcgcggc ggctcgccag gtggcggata
     5161 accctggcgg tgcctataac ccgttgttcc tttatggcgg cacgggtctg ggtaaaactc
     5221 acctgctgca tgcggtgggt aacggcatta tggcgcgcaa gccgaatgcc aaagtggttt
     5281 atatgcactc cgagcgcttt gttcaggaca tggttaaagc cctgcaaaac aacgcgatcg
     5341 aagagtttaa acgctactac cgttccgtag atgcactgct gatcgacgat attcagtttt
     5401 ttgctaataa agaacgatct caggaagagt ttttccatac cttcaacgcc ctgctggaag
     5461 gtaatcaaca gatcattctc acctcggatc gctatccgaa agagatcaac ggcgttgagg
     5521 atcgtttgaa atcccgcttc ggttggggac tgactgtggc gatcgaaccg ccagagctgg
     5581 aaacccgtgt ggcgatcctg atgaaaaagg ccgacgaaaa cgacattcgt ttgccgggcg
     5641 aagtggcgtt ctttatcgcc aagcgtctac gatctaacgt acgtgagctg gaaggggcgc
     5701 tgaaccgcgt cattgccaat gccaacttta ccggacgggc gatcaccatc gacttcgtgc
     5761 gtgaggcgct gcgcgacttg ctggcattgc aggaaaaact ggtcaccatc gacaatattc
     5821 agaagacggt ggcggagtac tacaagatca aagtcgcgga tctcctttcc aagcgtcgat
     5881 cccgctcggt ggcgcgtccg cgccagatgg cgatggcgct ggcgaaagag ctgactaacc
     5941 acagtctgcc ggagattggc gatgcgtttg gtggtcgtga ccacacgacg gtgcttcatg
     6001 cctgccgtaa gatcgagcag ttgcgtgaag agagccacga tatcaaagaa gatttttcaa
     6061 atttaatcag aacattgtca tcgtaaacct atgaaattta ccgtagaacg tgagcattta
     6121 ttaaaaccgc tacaacaggt gagcggtccg ttaggtggtc gtcctacgct accgattctc
     6181 ggtaatctgc tgttacaggt tgctgacggt acgttgtcgc tgaccggtac tgatctcgag
     6241 atggaaatgg tggcacgtgt tgcgctggtt cagcaacacg agccaggagc gacgaccgtt
     6301 ccggcgcgca aattctttga tatctgccgt ggtctgcctg aaggcgcgga aattgccgtg
     6361 cagctggaag gtgaacggat gctggtacgc tccgggcgta gccgtttttc gctgtctacc
     6421 ctgccagcgg cggatttccc gaacctcgat gactggcaga gtgaagtcga atttaccctg
     6481 ccgcaggcaa cgatgaagcg tctgattgaa gcgacccagt tttctatggc gcatcaggac
     6541 gttcgctatt acttaaatgg tatgctgttt gaaaccgaag gtgaagaact gcgcaccgtg
     6601 gcaaccgacg gccaccgtct ggcggtctgt tcaatgccaa ttggtcaatc tttgccaagc
     6661 cattcggtga tcgtaccgcg taaaggcgtg attgaactga tgcgtatgct cgacggcggc
     6721 gacaatccgc tgcgcgtaca gattggcagc aacaacattc gcgcccacgt tggcgacttt
     6781 atcttcacct ccaaactggt ggatggtcgc ttcccggatt atcgccgcgt tctgccgaag
     6841 aacccggaca aacatctgga agctggctgc gatctgctca agcaggcgtt tgctcgcgcg
     6901 gcgattctct ctaacgagaa attccgcggc gtacgtcttt atgtcagcga aaaccagctg
     6961 aaaatcaccg ccaacaaccc ggaacaggaa gaagcggaag agatcctcga cgttacctat
     7021 agcggtgcgg agatggaaat cggcttcaac gtcagctatg tgctggatgt tctgaacgcg
     7081 ctgaaatgcg aaaacgtccg catgatgctg accgattcgg tttccagcgt gcagattgaa
     7141 gatgccgcat cacagtcggc tgcctatgtt gtcatgccaa tgagactgta atgtccctca
     7201 cccgcttgtt gatccgcgat ttccgcaaca ttgaaaccgc ggatctcgct ttatctcccg
     7261 gctttaactt tctggtaggt gccaacggca gtggcaaaac cagcgtgctg gaagccatct
     7321 atacgctcgg ccatggtcgg gcgtttcgca gtttgcagat tggtcgcgtc attcgccatg
     7381 agcaggaggc gtttgttctc cacgggcgat tacagggcga agagcgcgag acagcgattg
     7441 gcttaaccaa agacaaacag ggcgacagca aagtccgcat cgacggtaca gacgggcata
     7501 aggtcgcgga actggcgcac ctgatgccaa tgcagttgat aacgccagaa gggtttactt
     7561 tactcaacgg cggccccaaa tacagaagag cattcctcga ctggggatgc tttcacaacg
     7621 aacccggatt tttcaccgcc tggagcaatc tcaagcgatt gctcaagcag cgcaatgcgg
     7681 cgctgcgcca ggtgacacgt tacgaacagc tacgcccgtg ggataaagaa ctgatcccgc
     7741 tggcggagca aatcagcacc tggcgcgcgg agtatagcgc cggtatcgcg gctgatatgg
     7801 ccgatacttg taagcaattt ctccctgagt tttctctgac tttctctttc cagcgcggct
     7861 gggagaaaga gacagaatat gctgaggtgc tggaacgtaa ttttgaacgc gatcgccagc
     7921 taacctacac cgcgcatggc ccgcataaag cggacttacg cattcgcgcc gacggtgcgc
     7981 cggtggaaga taccttatcg cgtgggcagc ttaagctgtt gatgtgcgcc ttacgtctgg
     8041 cgcaaggaga gttcctcacc cgtgaaagcg ggcggcggtg tctctacctg atagatgatt
     8101 ttgcctctga gcttgatgat gagcgtcgcg ggctgcttgc cagccgctta aaagcgacgc
     8161 aatcacaggt ctttgtcagc gcgatcagtg ctgaacacgt tatagacatg tcggacgaaa
     8221 attcgaagat gtttaccgtg gaaaagggta aaataacgga ttaacccaag tataaatgag
     8281 cgagaaacgt tgatgtcgaa ttcttatgac tcctccagta tcaaagtcct gaaagggctg
     8341 gatgcggtgc gtaagcgccc gggtatgtat atcggcgaca cggatgacgg caccggtctg
     8401 caccatatgg tattcgaggt ggtagataac gctatcgacg aagcgctcgc gggtcactgt
     8461 aaagaaatta tcgtcaccat tcacgccgat aactctgtct ctgtacagga tgacgggcgc
     8521 ggcattccga ccggtattca cccggaagag ggcgtatcgg cggcggaagt gatcatgacc
     8581 gttctgcacg caggcggtaa atttgacgat aactcctata aagtgtccgg cggtctgcac
     8641 ggcgttggtg tttcggtagt aaacgccctg tcgcaaaaac tggagctggt tatccagcgc
     8701 gagggtaaaa ttcaccgtca gatctacgaa cacggtgtac cgcaggctcc gctggcggtt
     8761 accggcgaga ctgaaaaaac cggcaccatg gtgcgtttct ggcccagcct cgaaaccttc
     8821 accaatgtga ccgagttcga atatgaaatt ctggcgaaac gtctgcgtga gttgtcgttc
     8881 ctcaactccg gcgtttccat tcgtctgcgc gacaagcgtg acggcaaaga agaccacttc
     8941 cactatgaag gcggcatcaa ggcgttcgtt gaatatctga acaagaacaa aacgccgatc
     9001 catccgaata tcttctactt ctccaccgaa aaagacggta ttggcgtcga agtagcgttg
     9061 cagtggaacg atggcttcca ggaaaacatc tactgcttta ccaacaacat tccgcagcgt
     9121 gacggcggta ctcacctggc aggcttccgt gcggcgatga cccgtaccct gaacgcctac
     9181 atggacaaag aaggctacag caaaaaagcc aaagtcagcg ccaccggtga cgatgcgcgt
     9241 gaaggcctga ttgcggtcgt ttccgtaaaa gtgccggacc cgaaattctc ctcccagacc
     9301 aaagacaaac tggtttcttc tgaggtgaaa tcggcggtag aacagcagat gaacgaactg
     9361 ctggcagaat acctgctgga aaacccaacc gacgcgaaaa tcgtggttgg caaaattatc
     9421 gatgctgccc gtgcccgtga agcggcgcgt cgcgcgcgtg aaatgacccg ccgtaaaggt
     9481 gcgctcgact tagcgggcct gccgggcaaa ctggcagact gccaggaacg cgatccggca
     9541 ctttccgaac tgtacctggt ggaaggggac tccgcgggcg gctctgcgaa gcaggggcgt
     9601 aaccgcaaga accaggcgat tctgccgctg aagggtaaaa tcctcaacgt cgagaaagcg
     9661 cgcttcgata agatgctctc ttcccaggaa gtggcgacgc ttatcaccgc gcttggttgt
     9721 ggtatcggtc gtgacgagta caacccggac aaactgcgtt atcacagcat catcatcatg
     9781 accgatgcgg acgtcgacgg ctcgcacatt cgtacgctgc tgttgacctt cttctatcgt
     9841 cagatgccgg aaatcgttga acgtggtcac gtctacatcg ctcagccgcc gctgtacaaa
     9901 gtgaagaaag gcaagcagga acagtacatt aaagacgacg aagcgatgga tcagtaccag
     9961 atctctatcg cgctggacgg cgcaacgctg cacaccaacg ccagcgcacc ggcattggct
    10021 ggcgaagcgt tagagaaact ggtgtctgag tacaacgcga cgcagaaaat gatcaaccgc
    10081 atggagcgtc gttatccgaa agcaatgctg aaagagctta tctatcagcc gacgctgacg
    10141 gaagccgacc tctctgatga gcagaccgtt acccgctggg tgaacgcgct ggtcagcgaa
    10201 ctgaacgaca aagaacagca cggcagccag tggaagtttg atgtccacac caatgccgaa
    10261 caaaacctgt ttgaaccgat tgttcgcgtg cgtacccacg gtgtggatac tgactatccg
    10321 ctggatcacg agtttatcac cggtggcgaa tatcgtcgta tctgcacgct gggtgagaaa
    10381 ctgcgtggct tgctggaaga agatgcgttt atcgaacgtg gcgagcgtcg tcagccggta
    10441 gccagcttcg agcaggcgct ggactggctg gtgaaagagt cccgtcgcgg cctctccatc
    10501 cagcgttata aaggtctggg cgagatgaac ccggaacagc tgtgggaaac caccatggac
    10561 ccggaaagcc gtcgtatgct gcgcgttacc gttaaagatg cgattgctgc cgaccagttg
    10621 ttcaccacgc tgatgggcga cgccgttgaa ccgcgccgtg cgtttattga agagaacgcc
    10681 ctgaaagcgg cgaatatcga tatttaatgg cgctaaccat gcgagcgtgc ctgatgcgct
    10741 acgcttatca ggcctacgaa aatcttgcaa tgtattgaat tttcatgatt ttgtaggccg
    10801 gataaggcgt tcacgccgca tccggcaaga agctataaga aaagggcgga gatcatctcc
    10861 gcccttttta tttctgcaat ccgcaattat catcccgcaa atctatactt tttgggtcaa
    10921 cacgtaacgc tttacaggtg attatcatgg ggctttttga tgaagttgtc ggtgcctttc
    10981 tgaaaggcga tgcggggaaa tatcaggcta ttttaagttg ggttgaggag cagggcggca
    11041 ttcaggtgct gctggaaaaa ctgcaaagtg gcggcttagg ggccattctc tcaacctggc
    11101 tgagtaatca acagggcaat caatcggtta gtggcgagca actggaatcg gcgctcggca
    11161 caaatgcggt gtccgatctt ggacaaaaac ttggcgtgga taccagtaca gcttccagtt
    11221 tactggcaga acaattgccg aagattattg atgcgctctc accgcaaggt gaagtgtctc
    11281 cacaagccaa taacgatctg ctttccgcag gcatggaact gctgaaaggg aaactcttcc
    11341 gctaagcaaa tggggagcat gcaacccgcg ttgcatcccc cttttccctc gggtgttgta
    11401 ctgatttttg agcggaatcg cgttagcatg ggtcaggaac taatctacct ggggaactca
    11461 tggctatcaa actcattgct atcgatatgg atggcaccct tctgctaccc gatcacacca
    11521 tttcacccgc cgttaaaaat gcgattgccg cagctcgcgc ccgtggcgtg aatgtcgtgc
    11581 taacgacggg tcgcccttat gcaggtgttc acaattacct gaaagagctg catatggaac
    11641 tgccgggcga ctactgcatt acttataacg gcgcgctggt acagaaggcc gctgatggta
    11701 gcaccgtggc gcaaactgct ctcagctatg acgactaccg tttcctggaa aaactctctc
    11761 gcgaagtcgg ttctcatttc cacgccctgg accgcaccac gctgtacacc gccaaccgtg
    11821 atatcagcta ctacacggtg catgaatcct tcgttgccac aattccgctg gtgttctgcg
    11881 aagcggagaa aatggacccc aatacccagt tcctgaaagt gatgatgatt gatgaacccg
    11941 ccatcctcga ccaggctatc gcgcgtattc cgcaggaagt gaaagagaaa tataccgtgc
    12001 tgaaaagtgc gccgtacttc ctcgaaatcc tcgataaacg cgttaacaaa ggtacggggg
    12061 tgaaatcact ggccgacgtg ttaggtatta aaccggaaga aatcatggcg attggcgatc
    12121 aggaaaacga tatcgcaatg attgaatatg caggcgtcgg tgtggcgatg gataacgcta
    12181 ttccttcggt gaaagaagtg gcgaactttg tcaccaaatc caaccttgaa gatggcgtgg
    12241 cgtttgctat tgagaagtat gtgctgaatt aatcagtgga cgggcaaaca gcataaacgc
    12301 gtttgcccgc ttactgatta ttggtttact gcatatacaa ccagaaattg cagcggtgca
    12361 gtaatgatgt ccagcgtaac agtcactggc agtaatgctg cggaaacacc atccggaatg
    12421 cctctgggtg aatagtattc ataaaatcgc actttaaacg gatgatagaa cgccatcacc
    12481 tttgagtaat ccatgttttt atttttctca tggattgtgc cttttaggtt ttccagtgac
    12541 ctggtacagt gttgaacacc acaggcaaaa ccataatgcg ttgccagtgc cttttcttct
    12601 tcgttattcc agtagtagta aagcgatatt gtgccggtaa atttcttttt ccccgcattt
    12661 aaaacaaatc ttgcgtcatc ggcaacctga atattgtgcc ggttaagtgc tggatcattg
    12721 agcatcttaa cgatgtcatc ggcaccatca gtgataaggt aatccagcga ttcccctaca
    12781 aaaacatagc cttttgttcc attactatct tttgcctgcg atagcccagt gatggtgtct
    12841 gactgcgatt gatactttcc tgaactaccc tttgcggtat gggaaaccag agcatacgag
    12901 atacagcctg aaagcatcag cgccagtggg aataaaccgg cagccttaaa aaatccctta
    12961 aaattcaact tcatgattaa tcctttaaac gagtagctaa gccgttgaaa gcatactctt
    13021 ctttccctcc tgtttagcgc ctgatatccc ttttcagatt tctgcccgac gcatgtcatt
    13081 tttttatgca ttgttctttt tgtgatctaa attgtagtac aacaatataa gtttgtacta
    13141 cattacgcac acggcaaacg cgaacgtcat cacgctggta ctacaaagtt gccgcgttat
    13201 gcatcgatcg gggtaaagta gagaagaaca tacagagcac aaggactctc catgactctc
    13261 aataaaaccg atcgcattgt cattacgctg ggtaaacaga tcgttcacgg caaatacgta
    13321 cctggctcgc cacttccggc tgaggcggag ctctgtgaag agtttgcaac ctcgcgcaac
    13381 atcatccgtg aggtgttccg ttcgctgatg gcgaagcggc tgattgaaat gaaacgttat
    13441 cgcggggcgt ttgtggcacc gcgtaaccag tggaattacc tcgacactga cgtactgcaa
    13501 tgggtgttgg aaaacgacta cgacccacgg cttatcagtg ccatgagcga agtgcgaaat
    13561 ctggtggaac cggcgattgc ccgttgggca gcagagcgcg cgacctccag cgatctggcg
    13621 cagattgaat cggcgctgaa cgagatgatt gccaacaatc aggaccgcga agcgtttaac
    13681 gaagcggata ttcgctacca cgaggcggtg ctgcagtcgg tgcataaccc ggtgttacag
    13741 caacttagca ttgcgatcag ttcgctgcag cgggcggttt ttgaacgaac ctggatgggc
    13801 gatgaggcca acatgccgca aacgctccag gaacataagg cgctgttcga tgcgatacgg
    13861 catcaggacg gcgatgcggc agagcaggcg gcgcttacca tgatcgccag ctcgacacga
    13921 aggttaaagg aaatcacatg acagctcgct acatcgcaat tgactgggga tcgaccaatc
    13981 ttcgcgcctg gctttatcag ggcgaccact gcctggagag caggcaatca gaagcaggcg
    14041 tcacgcgcct gaacggaaaa tctccggctg cggtgttagc agaagtcacg accgactggc
    14101 gtgaagagaa tacgccagtg gtaatggcag gaatggtcgg cagcaatgtc ggctggaaag
    14161 ttgctccgta tttatctgtt cctgcccgtt tttcgtctat tggcgaacaa ttaacgtctg
    14221 ttggcgacaa tatctggatt attcccggat tatgcgtctc tcatgacgat aaccacaatg
    14281 tgatgcgcgg cgaagaaaca caattgatcg gcgcgcgaac tctggctcct tcctctcttt
    14341 atgttatgcc cggtacgcat tgcaaatggg tgcaggccga tagccagcaa atcaacgatt
    14401 ttcgcaccgt gatgaccggt gaactacatc atttactgtt aaatcactca ttgattggcg
    14461 caggtttgcc gccgcaggaa aactctgccg atgccttcgc ggctggcctt gagcgcggcc
    14521 ttaatgcgcc cgccatattg ccgcagcttt ttgaagttcg cgcctcgcat gtgctgggaa
    14581 cacttccccg cgaacaggtc agcgaatttc tctctggttt gttgattggc gcagaggttg
    14641 ccagtatgcg cgactatgtg acccatcaac acgccatcac ccttgtcgcc ggaacatcgc
    14701 tgaccgcgcg ctaccagcaa gcctttcagg cgatgggttg cgacgtgacg gcggtggcgg
    14761 gcgacacggc atttcaggct ggtataagga gcatcgctca tgcagtggca aactaaactt
    14821 ccgctgatcg ccattttgcg cggcattaag cccgacgagg cgctggcgca tgttggcgcg
    14881 gtgattgacg ccgggttcga cgcggttgaa atcccgctga attccccaca atgggagcaa
    14941 agcattcccg ccatcgttga tgcgtatggc gacaaggcgt tgattggcgc aggtacggta
    15001 ctgaaacctg aacaggtcga tgcgctcgcc aggatgggct gtcagctcat cgttacgccc
    15061 aatatccata gtgaagtgat ccgccgtgcg gtgggctacg gcatgaccgt ctgccccggc
    15121 tgcgcgacgg cgaccgaagc ctttaccgcg ctcgaagcgg gcgcgcaggc gctgaaaata
    15181 tttccgtcat cggcttttgg tccgcaatac atcaaagcgt taaaagcggt attgccatcg
    15241 gacatcgcag tctttgccgt tggcggcgtg acgccagaaa acctggcgca gtggatagac
    15301 gcaggttgtg ctggggcggg cttaggcagc gatctctatc gcgccgggca gtccgtagaa
    15361 cgcaccgcgc agcaggcagc agcatttgtt aaggcgtatc gagaggcagt gcaatgaaaa
    15421 tcaccaaaat taccacgtat cgtttacctc cccgctggat gttcctgaaa attgaaaccg
    15481 atgaaggcgt ggtcggttgg ggcgagcccg tgattgaagg ccgcgcccgt acggtggaag
    15541 cggcagttca cgagctgggt gactatttga ttggtcagga tccttcgcgc atcaatgact
    15601 tatggcaagt gatgtatcgc gccggatttt atcgtggcgg tccaatcctg atgagcgcca
    15661 ttgccgggat cgaccaggcg ttatgggata tcaaaggcaa agtgctgaat gcgccggtct
    15721 ggcaactgat gggcggcctg gttcgcgaca aaattaaagc ctacagttgg gtcggcggcg
    15781 atcgtccggc ggatgttatc gacggcatta aaaccctgcg cgaaatcggc ttcgatacct
    15841 tcaaactgaa cggttgtgaa gaactggggc taattgataa ctcccgcgcg gtagatgcgg
    15901 cagtcaacac cgtggcacaa attcgtgaag cttttggcaa tcagattgag tttggtcttg
    15961 atttccacgg tcgcgtcagc gcgccaatgg cgaaagtgct gattaaagaa ctggagccgt
    16021 atcgcccgct gtttattgaa gagccggtgc tggcggaaca agccgaatac tacccgaaac
    16081 tggcggcaca aacgcatatt ccactggcgg cgggtgagcg catgttctca cgtttcgatt
    16141 ttaaacgcgt gctggaggca ggcggtattt cgattctgca accggatctc tcccatgcag
    16201 gcggtattac cgaatgctac aaaattgctg gaatggcaga agcctatgat gtgacccttg
    16261 cgccgcactg tccgctcgga ccgattgcac tggcggcttg cctgcatatc gactttgttt
    16321 cctataacgc ggtacttcag gaacaaagta tgggcattca ttacaacaaa ggcgcggagt
    16381 tactcgactt tgtgaaaaac aaagaagact tcagtatggt tggcggcttc tttaaaccgt
    16441 taacgaaacc gggcttaggt gtggaaatcg acgaagctaa agttattgag ttcagtaaaa
    16501 atgccccgga ctggcgtaat ccgctctggc gtcatgaaga taacagcgta gcagagtggt
    16561 aattcctgcc acgtaagccc ctcatcgggc actaaaacag caatacaaaa atataaccct
    16621 ctgtaaatta cagggcatgg tgagcggctt cgctatgccc aaaatctgga gaaagctcgc
    16681 tatggatatt cccgttaatg cagcaaagcc ggggcgtcgg cgttatctga cgctggtgat
    16741 gatctttatt acggtggtca tttgttatgt tgaccgcgct aacctggccg tggcttccgc
    16801 ccatattcag gaagagttcg gcattacgaa agcggaaatg ggctatgtat tttcggcctt
    16861 cgcctggctt tatacgctat gccagatccc cggcggttgg tttttagatc gcgtaggttc
    16921 tcgcgtgact tattttattg cgatatttgg ttggtcagtg gcgactttat tccagggctt
    16981 tgccacggga ttaatgtcat taattggtct gcgtgcgata accggtattt tcgaagcgcc
    17041 cgctttcccg accaataacc ggatggtgac cagctggttc ccggaacatg aacgcgcttc
    17101 cgccgttggt ttttatacgt ctggtcagtt tgtcggtctg gcgtttttga ctccgctgct
    17161 gatctggatt caggagatgt tgagctggca ctgggtgttc attgtcactg gtggtatcgg
    17221 cattatctgg tcgctgattt ggtttaaggt ttatcagccg ccgcgcctga ccaaaggtat
    17281 cagcaaagct gaactggatt acattcgtga tggcggcggt ctggtggatg gtgatgcgcc
    17341 ggtgaagaaa gaggcgcgtc agccgttaac agccaaagac tggaaactgg tgttccatcg
    17401 taaactgatc ggcgtttatc ttgggcaatt tgcggtggct tctacactgt ggtttttctt
    17461 aacctggttc ccgaactatt taacccagga aaaaggaatc acggcgctga aagcaggctt
    17521 tatgaccacg gtgccattcc tcgcggcgtt tgtcggcgtc ctgctctctg gctgggtcgc
    17581 ggatctgctg gtacgtaagg gcttttcact gggctttgcg cgtaaaacgc cgattatctg
    17641 cggcttgctg atctccacct gcattatggg cgctaactac accaacgatc cgatgatgat
    17701 tatgtgcctg atggcgctgg cattcttcgg caacggtttt gcttcgatta cctggtcgct
    17761 ggtctcttct ctggcaccga tgcgcctgat tggtttaacc ggcggcgtgt ttaacttcgc
    17821 cggtggtctg ggcggcatca ccgttccgct ggtggtgggg tacctggcgc agggttacgg
    17881 tttcgcacct gcactggttt atatctccgc cgtcgcgttg attggcgcgc tctcttacat
    17941 cctgctggtg ggcgatgtga agcgcgttgg ctaatctttc aactgtggaa tatgcgccat
    18001 accactgcgc ataatccact ttcttaaagc cggtgcggtc aggcatcggc tttttactat
    18061 cttgccgaag agttttaaac gcagtttgcg ggtggcacgc cagtaagcgg tattgagctt
    18121 ctctggctgc ttcagtaaca ccgcacgcag aatgtctgcg ctatccagcg catagctaat
    18181 cccctccagc gaactggcgc tgataaatcc tgccgcttcg ccaatcagaa aggcgttgtc
    18241 cttaccgcag acaaaatcct gccagcgtga gggaaacagc accgtgcatt tttcgctttt
    18301 caccgcctta ccaaactgga actgaaaggc gctcattttc tctttcagcg tcgtgaaacg
    18361 cgtctgaccg tctttcattg gataagcacc gccaaagata aaataaccgt ctttgctgat
    18421 actccatgaa taacagttag ttatcgaatt atcaaagatg caggagtaga acggcaccgg
    18481 atgtttctcc gcgaaccact gctggatagc gacatattta cggatttgat gatccgggta
    18541 gagatgtcgc cgcaccatcg agtttgcgcc atcggcaccg accagatagc gggcggtaat
    18601 atgttgctcc cagccgtctg cacgaaaaat gacatgccat ttatcatcct cacgccagat
    18661 gtttcggcac aggctatcgt gataaacctc aacgctggtg gggatcagcg atttcatcca
    18721 caagtcgaaa gcatggcgat taatattgat atagcttcgc tggtagttac gcgtgagcga
    18781 tgcggcgacg tcgacagttt tgacgctgaa aatctgtgga ttggcgatca catcgacagg
    18841 aagcgtcagt ccatcgcgaa taaaagatcg ctgcgcgtcc ggtgccagca gaccgccgca
    18901 aggtttgctg aaaccttcag taccacactg gtgcttttta tccagcgcga tcacctgcat
    18961 tttgcctgct aattttcgcg ccaacgccga tcctgccggg ccgaggccaa taatcgccac
    19021 gtcgaaatgt tccatctttt cactcacatt catcacgatg gcaaaagact attgtctgaa
    19081 agtgaagtcc ttgtgaagta tgatggtggg acccgttttg tatcaggacc gcgccatgaa
    19141 acagataacc tttgctcccc gtaatcacct gctcaccaat accaatacct ggacgcccga
    19201 cagccagtgg ctggtatttg acgtgcgtcc ttctggggcg tcgtttaccg gcgagaccat
    19261 tgagcgtgtg aatatccata ccggcgaggt cgaggttatc tatcgcgcgt cacagggcgc
    19321 acacgtcggc gtggtgaccg ttcatccgaa gtcagagaaa tatgttttca tccatggccc
    19381 ggaaaatcct gatgaaacat ggcattacga tttccatcat cgacgcggag tcattgctga
    19441 aggcggcaag gtgagcaatc tcgatgcaat ggatattacc gcgccgtata cgccaggagc
    19501 gctgcgcggc ggcagccatg tgcatgtctt tagcccgaac ggtgaaaggg tgagctttac
    19561 ctataacgac catgtaatgc atgaactcga tccggcgctg gatttgcgaa acgtcggtgt
    19621 tgctgcgccg tttggcccgg tcaacgtaca aaagcagcat ccgcgtgaat acagcggtag
    19681 ccactggtgc gtgctggtga gtaaaaccac gcccacgccg cagcctggca gcgatgaaat
    19741 caatcgtgct tatgaagaag gatgggtagg aaatcacgcg ctggcgttta ttggcgacac
    19801 actttcgcca aagggcgaga aagtgccgga gctgtttatc gttgagttac cgcaagatga
    19861 agctggctgg aaagcggcag gtgatgcgcc gttaagtgga acggaaacca ccctgcccgc
    19921 gccaccgcgc ggcgtcgtgc agcgacgttt aacctttacc caccatcggg cttatccggg
    19981 gttagtcaac gtcccgcgcc actgggtgcg ctgtaatccg cagggtacgc aaatcgcgtt
    20041 tttaatgcgt gatgataacg gcattgtgca actgtggctt atctcgccac agggcggcga
    20101 gccgcgccag ttaacccata acaaaacgga tattcagtct gcatttaact ggcatccgtc
    20161 aggagaatgg ttgggctttg tgctggataa tcgaattgct tgtgcccatg cgcaaagcgg
    20221 cgaggttgag tatttaaccg aaaaccacgc caatccacct tctgcggacg ccgtggtctt
    20281 ctcgccggat ggtcagtggc tggcgtggat ggaaggcggc cagctgtgga tcaccgaaac
    20341 tgatcgctaa atcagttatc aggcattctg gccggtggaa taaccgcatt ggtggcttgc
    20401 gcgttggctt cgcttttttc aacgcgtgag cgcaccgaac tgtctttgcg gaacacatcc
    20461 cacggcagta aaagcgtgtc catcacagcg gtaaatggca tatcgagaat cgccagcgac
    20521 ttagtgcccc agttggtttc atcgtcgcca atcatggttg cgctggcgcg ggtgccggga
    20581 tacgttcctt ctttccctcc ggtatgggac atcacgctgg agcatcctcc gagtaatgtc
    20641 attccgctgc atatcatgaa cgctaacaac acatttctta tcataataaa ttcatctgtt
    20701 gatcgtgggt gttggcctga tgagttatag cgatcccttg ctgaaaataa catcatcatt
    20761 acgtcgcact gtggcggcta tcgcacttta acgtttcgtg ctgccccctc agtctatgca
    20821 atagaccata aactgcaaaa aaaagtccgc tgataaggct tgaaaagttc atttccagac
    20881 ccatttttac atcgtagccg atgaggacgc gcctgatggg tgttctggct acctgacctg
    20941 tccattgtgg aaggtcttac attctcgctg atttcaggag ctattgatta tgcgtaactt
    21001 tgatttatcc ccgctttacc gttctgctat tggatttgac cgtttgttta accacttaga
    21061 aaacaaccag agccagagta atggcggcta ccctccgtat aacgttgaac tggtagacga
    21121 aaaccattac cgcattgcta tcgctgtggc tggttttgct gagagcgaac tggaaattac
    21181 cgcccaggat aatctgctgg tggttaaagg tgctcacgcc gacgaacaaa aagagcgcac
    21241 ctatctgtac cagggcatcg ctgaacgcaa ctttgaacgc aaattccagt tagctgagaa
    21301 cattcatgtt cgtggcgcta acctggtaaa tggtttgctg tatatcgatc tcgaacgcgt
    21361 gattccggaa gcgaaaaaac cgcgccgtat cgaaatcaac taattcccta aggccgcctg
    21421 gcgcggcctg acatctccat gctcgccgtc agggagcata tgcgaatctt cggatttgca
    21481 ggtacttact cgcttcttag aaggagaaat gactatgcgt aacttcgatt tatccccact
    21541 gatgcgtcaa tggatcggtt ttgacaaact ggccaacgca ctgcaaaacg ccggtgaaag
    21601 ccagagcttc ccgccgtaca acattgagaa aagcgacgat aaccactacc gcattaccct
    21661 tgcgctggca ggtttccgtc aggaagattt agagattcaa ctggaaggta cgcgcctgag
    21721 cgtaaaaggc acgccggagc agccaaaaga agagaaaaaa tggctgcatc aagggcttat
    21781 gaatcagcca tttagcctga gctttacgct ggctgaaaat atggaagtct ctggcgcaac
    21841 cttcgtaaac ggtttactgc atattgattt aattcgtaat gagcctgaac ccatcgcagc
    21901 gcagcgtatc gctatcagcg aacgtcccgc gttaaatagc taactagcta ttctctttgc
    21961 cccgccattc tggtggggct ttttttgtct ttcttcttca acaatgcgtt aaatttcgcc
    22021 tgtttaagat atttcggcac gttctttccc gtgataatgt gcgctataag gcaaatctgc
    22081 ttcaccgatt cagccataat ccgtagcaat taaaaatgtt atctcaagag aaatggacga
    22141 tgagtgatat agcattaacg gtcagtattc tggctttggt ggcagtcgtc ggtttgttta
    22201 tcggcaatgt caaatttcgc ggcataggat taggtattgg cggcgtgctg tttggtggga
    22261 tcatcgtcgg ccattttgtt tctcaggcgg ggatgacatt aagtagcgat atgctgcatg
    22321 ttattcagga atttggcctg atcctgttcg tttatactat cgggattcag gtagggccgg
    22381 gcttctttgc ctcattgcgc gtctccggat tacgcctcaa cctgtttgct gttctgatcg
    22441 tcatcatcgg tggtctggtt accgccatcc tgcataaact gtttgatatt ccactgccgg
    22501 tagtgctggg gattttctcc ggtgcggtta ccaatacgcc agcgctgggg gcagggcagc
    22561 agattttgcg cgacctgggt acaccaatgg aaatggtcga tcagatgggg atgagttacg
    22621 cgatggcgta tccattcggc atttgcggga ttttgttcac catgtggatg ttgcgggtta
    22681 ttttccgcgt caatgtcgag acagaagctc agcagcacga gtcttcacgc accaatggcg
    22741 gcgcgctgat caagactatc aatattcgcg ttgagaaccc taacctgcat gatttagcca
    22801 ttaaagatgt accgattctc aacggcgaca aaattatctg ctcgcgtctg aaacgcgaag
    22861 aaaccctaaa agttccttcg ccagatacca ttattcaact gggcgatttg ctgcatctgg
    22921 tgggtcagcc agcggattta cataatgcgc aactggtgat tggtcaggag gtcgatactt
    22981 cgctgtccac gaaaggcact gatttgcgcg tcgagcgtgt ggtggtcacc aatgaaaacg
    23041 tgctcggaaa acgtattcgc gacctgcact ttaaagaacg ctatgacgtt gttatctcgc
    23101 gcctgaaccg tgccggggtc gaactggtcg ccagtggcga tatcagcctg cagttcggcg
    23161 atatcctcaa tctggtgggg cgtccgtccg caattgatgc cgttgccaat gtgctgggga
    23221 atgcgcagca aaaactgcaa caggttcaga tgctgccagt gtttattggc atcgggcttg
    23281 gcgtattgtt aggttctatt cccgtctttg tgcctgggtt cccggccgcg ttgaaactgg
    23341 ggctggcggg cggtccgctg attatggcgt tgatcctcgg gcgtatcggc agtatcggca
    23401 agctgtactg gtttatgccg ccaagtgcca acctcgcgct gcgggagctg gggatcgtgc
    23461 tgttcctctc ggtcgttggt ctgaaatctg gtggggattt tgtgaatacc ctggtcaatg
    23521 gcgaagggct aagctggatt ggttatggtg ccctgatcac cgccgttccg ctgattactg
    23581 ttggcattct ggcgcggatg ttagccaaaa tgaattacct gaccatgtgc gggatgctgg
    23641 caggttccat gaccgatcct ccggcgctgg cgtttgctaa taatcttcat ccaaccagtg
    23701 gtgcggcggc gctctcttac gccactgtct atccgttagt gatgtttctg cgcattatca
    23761 ccccccaatt actggcggtg ctcttctgga gtatcggtta actcttttcc gggtggagtc
    23821 gccgtaaatg gtactccacc tggtattcgc tggcatttct gaacatcact gaataattca
    23881 aaaactcccc gctctcgcta tatgacaacg aggtgatccg cagcagtggc gtgtgttccg
    23941 ctaccttcat ttgccgcgcc agcagtctat cggcgagcac cggcgtcagg ctttcataat
    24001 taccgccaat caaaatcccg cactcttgtc caatatattc aaacttcgac ccttccagat
    24061 gttgcagcga aagattacgg aacagtttaa ccggcatata gctgtcttcc agcatcagcg
    24121 gtttcccttc cacaaaacga acgcgacggg agaagtagat ctgctcgttg atttgaatcc
    24181 gtaactggct ggcaatggcc ggaggcgcag gcatgatttc aaaaatcagc acctggctgg
    24241 tgaccgtttt tccctgccgt tttaacacct ccaccagtcc ggtcaggctg gcggtttgat
    24301 gcagcacatc tttgcgcacc aggtaagtgc cgctgccgtg gcggcggacc accagccccc
    24361 acgctaccag caggtcaatc gctttacgga tggtcatccg ggatactgca aattcttccg
    24421 ccaacttttt ttcaccggga agaaggctgt ttagcgtgaa atccgcgtaa ttaagtcgaa
    24481 ttcttaaccg ctccgcaatg cttttgtaga tcatctctag atctcttttt tgcctgcaag
    24541 cccgggtaat gcgcccacaa gttctgctta atcgattgaa aaaacatctt ttttttaaag
    24601 atgtgttcga tgctgtgtac cagtgctcac agatgtctac tttttcgcga aaacgtagat
    24661 ctctaccgcc caacgaaaag catgaaagcg atcacgaatc ccattgggtc gtcatgttct
    24721 gccgatcgca tcttcctatc ctcgctccag gcctgccgca taaccaatca ggcttcctac
    24781 ttacagaatt gagaaaagag gatgtggaaa tgctcagtca aattcaacgc tttggcggcg
    24841 cgatgttcac gccagtgctg ctgtttccct tcgccgggat tgtggtgggt cttgccatct
    24901 tgctgcaaaa cccgatgttt gtcggggaat cactgaccga tccgaacagt ttattcgcgc
    24961 aaatcgtaca cattattgaa gagggcggtt ggacggtatt ccgtaatatg ccgctgattt
    25021 ttgctgtcgg tttacccatt ggccttgcta agcaagcgca ggggcgtgct tgtctggcgg
    25081 tgatggtgag tttcctgacc tggaactatt tcatcaacgc gatgggaatg acctggggaa
    25141 gctacttcgg cgtcgatttc actcaggacg cggtggcagg tagcggtctg acaatgatgg
    25201 ccgggattaa aaccctcgat accagcatta tcggcgcaat tatcatttcc ggcattgtga
    25261 cggcgctgca taaccgtctg ttcgataaaa aactgccggt ttttctcggc attttccagg
    25321 gaacgtctta tgtggtgatt atcgccttcc tggtgatgat cccctgtgcc tggctgacat
    25381 tgctcggctg gccaaaagta caaatgggga ttgaatctct gcaagcgttc ctgcgttcgg
    25441 cgggtgcact tggggtctgg gtttacacct tcctcgaacg tattctgatc ccaaccggtt
    25501 tacaccactt catctacgga ccgtttatct ttggtctggc agctgttgaa ggcggcattc
    25561 agatgtactg ggcgcagcat ctgcaagagt tcagtctgag cgccgagccg ctgaaatcgt
    25621 tgttcccgga aggcggtttt gccctgcacg gtaactcaaa aatctttggt gccgtgggca
    25681 tttctttagc gatgtacttc actgccgcac cggaaaatcg ggtaaaagtg gcgggcttgc
    25741 tgattcccgc aaccttaacc gccatgctgg tgggaattac cgaaccgctg gaatttacct
    25801 tcctgttcat ttcaccgttg ctgtttgcgg tacacgctgt gcttgcggcc tcaatgtcga
    25861 ccgtgatgta tctctttggt gtggtgggca acatgggcgg aggtctgatt gaccaggttt
    25921 taccgcaaaa ctggatcccg atgttcagca accacgcgga tatgatgctg acccaaatcg
    25981 ccattgggtt gtgctttacc ctgctgtact tcgtggtttt ccgcacactg attctgcagt
    26041 tcaacatgtg cacgccggga cgtgaagatg cggaagtgaa actctactca aaagccgaat
    26101 acaaagcctc gcgaggccaa accaccgcgg cagagccaaa aaaagagctg gatcaggctg
    26161 ccggtatcct gcaagccctg ggcggggtcg gcaatatctc cagcattaac aattgtgcga
    26221 cgcgtctacg cattgcactg catgacatgt cacaaacgct ggatgacgaa gtctttaaaa
    26281 agctgggagc gcacggcgtc ttccgtagtg gcgatgccat tcaggtgatc attggtctgc
    26341 atgtatccca gctgcatgaa cagctcgata gcttaattaa ttctcatcaa tcagcagaaa
    26401 atgttgccat tacggaggca gtataatgac caaattctca gtggttgtcg caggcggtgg
    26461 aagcaccttt acgccaggca tcgtgttgat gctcctggcg aatcaggagc gtttcccgct
    26521 tcgtgcactg aaattttatg ataacgatgg cgcgcggcag gaagtgattg ccgaagcctg
    26581 taaagtcatc cttaaagaga aagcgccgga cattgcgttt agttacacca ccgatcctga
    26641 agtggcgttc agcgacgttg attttgtcat ggcgcacatc cgcgtcggca agtacccgat
    26701 gcgcgaactg gatgaaaaaa tcccactgcg ccacggtgtt gttggtcagg aaacttgcgg
    26761 acccggcgga atagcgtacg gcatgcgttc cattggcggc gtcctggaac tggtggatta
    26821 tatggaaaaa tattcaccaa atgcctggat gctcaactac tccaacccgg cagccattgt
    26881 cgcagaagcc acgcgtcgtc tgcgcccgaa tgcgaaaatc ctcaacatct gtgacatgcc
    26941 aatcggtatt gaaagccgga tggcgcaaat tgttgggctg caagatcgca aacagatgcg
    27001 cgtgcgctac tacggcctga accactggtg gtcggcgata tcccgcagtt tcagaaaggg
    27061 ttaatgagtc agcaagtggc ggtggaaaaa ctggtcgtgg acgcctggga acagcgttca
    27121 tatcagcacc tgtggcaggc gattacgttg tcgaaaacgg taccgagcgc ctcggtcgcc
    27181 aaagctattc tggatgagtt gctggaggcc aacaaagcgt actggccaga gttacgttaa
    27241 ttgtgtggac tggcatccgt tgggtgctgg tccgtattct ttagcacggc tgacggcgca
    27301 taaccaaatt tatgtttaaa ggctttgctg aaatgaaagg agtcgaagaa gttgagcgta
    27361 tcagcgactt cgccaaccga gtttccttgt tgctttaata gcgatagcgc cagatccaga
    27421 cgcgcatcga ggtaatattc tttcggcgtc ttgccggtat agcgtaaaaa cagacgacgc
    27481 aaccaggctt cgctacaggg gatcgtggca gccatatcag ctacgctcca gcgttgttgc
    27541 agactggcat gtaaagtggc aattaatttt tcaatttgcc gccgttgtgg atctttttta
    27601 ccatctgcgt acataagaca aatccactga taaataattt tcgtcagaaa agcgactgcc
    27661 agattatttt ttattgcttc tggtgaagtt attaactcag caacttcggt gagttcctga
    27721 ttataaattt cgccattata aataacgctt tgctgaccaa cgggaatatc catcatactg
    27781 gtgggggtaa attccatcca gtactgttcc cagactaaac cttcacagtg ataagagtga
    27841 atatccattg gctttaagaa tataatacag ttgccatgca gtgttatttg ttcgccattc
    27901 tttaaaagaa ttttcccaca tccttgtacg gtatacaccg caacccatga gttggcgata
    27961 agcggtttct tcttgtcccg cggccagatc acccggtaat gctcgtcggc gtaggtatgc
    28021 cagagcgaga tcagagaaat tccgctggag atcacatttt cgttaatcaa taagggaata
    28081 ttgtacggag tttcgttttt tacatctgaa ctttgcaatt ttccattcat tttaatatcc
    28141 ctccggcgaa ttatgctcac aacctgaact taacagaaaa caatccgttc agggatacga
    28201 cgtttgtatt ttaagaatct gactgcctga cccgacgcat tttacctctc cctatattca
    28261 tgcgtccggg acataaataa gggctatgag atgaattcgt tacaaatctt gagttttgtc
    28321 ggttttacgc tgctggtggc ggtgatcacc tggtggaagg tccgcaaaac agataccgga
    28381 tcgcaacaag gctattttct tgccggacgt tcactaaaag cgccggttat tgccgcttcg
    28441 ttaatgctaa ccaacctttc cacggaacaa ctggtcggcc tttccgggca ggcctacaaa
    28501 agcggcatgt cggtgatggg ctgggaagtg acttcagcgg tgacgctgat cttcctcgcg
    28561 ctaatctttt taccgcgcta tctgaagcgc ggcattgcca ccatccccga ttttctggag
    28621 gaacgttatg ataaaacgac gcgtattatc atcgacttct gcttcctcat tgccaccggc
    28681 gtctgctttc tgccgattgt tctctactcc ggcgcgttgg cgctcaacag cctgtttcac
    28741 gtcggggaat cgctacagat ttctcacggt gcggctatct ggctactggt aattttgctt
    28801 ggtctggcgg gaattttgta tgcggtgatc ggcggactgc gcgcaatggc agtggcggac
    28861 tccatcaacg gtattgggct ggttatcggc gggttgatgg tgccggtatt tggcctgatc
    28921 gcgatgggca agggcagctt tatgcagggc attgagcaac tcaccaccgt tcacgccgag
    28981 aaattaaact caatcggtgg cccgaccgat cccttgccga ttggcgcggc atttaccggt
    29041 ttgattctgg tgaacacctt ttactggtgt acaaatcagg gcatcgtgca acgcacgctg
    29101 gcgtcaaaaa gcctggcgga agggcaaaag ggggcgctgt taacggcggt gctgaaaatg
    29161 ctcgacccgc tggtactggt gctgccaggg ttgattgcgt ttcatctgta tcaggattta
    29221 ccgaaagccg acatggccta cccgacgctg gtcaataacg ttctgccagt gccaatggtg
    29281 ggtttcttcg gcgcggtgtt atttggtgcg gtgatcagta ccttcaacgg ctttctgaat
    29341 agcgccagta cgttattcag tatgggtatt taccgtcgca tcattaacca gaatgccgag
    29401 ccgcagcagc tggtcaccgt cgggcgcaaa tttggtttct ttatcgctat cgtttcggtg
    29461 ctggtcgcgc cgtggatcgc caacgcgccg caggggctgt atagctggat gaaacagctc
    29521 aacggcattt acaacgtgcc gctggttacc atcatcatta tgggcttttt cttcccgcgc
    29581 atcccggcgc tggcggcaaa agtggcgatg gggattggca taatcagcta catcaccatc
    29641 aactatctgg tgaagttcga cttccatttc ctctatgtgc tggcctgtac gttctgcatc
    29701 aacgtggtcg tgatgctggt gatcggtttt atcaaaccgc gcgccacgcc gttcaccttc
    29761 aaagatgcgt ttgcggtgga catgaaaccg tggagaaacg tcaagatcgc gtcaattggc
    29821 atcctgttcg cgatgattgg cgtctatgcc gggctggctg aattcggcgg ctacggtacg
    29881 cgctggttag cgatgatcag ttatttcatt gccgccgtag tgattgtcta cctgattttt
    29941 gacagctggc ggcatcgtca cgacccagcc gtaaccttta ctcccgacgc gaaggatagc
    30001 ctatgaaacg ccccaatttt ctgttcatca tgaccgatac ccaggccacc aatatggtcg
    30061 gttgctatag cggtaaaccg ctgaatacgc aaaatattga tagtctggcg gcggaaggta
    30121 ttcgctttaa ttccgcctac acctgttcac cggtttgtac gcctgcacgc gccggactat
    30181 ttaccggtat ctacgctaac cagtccggcc cgtggaccaa caacgtcgcg ccaggcaaaa
    30241 acatctccac tatggggcgc tactttaagg atgccggcta tcacacctgt tacatcggca
    30301 aatggcatct cgacggtcat gactatttcg gcactggcga gtgtccgccg gagtgggacg
    30361 ctgattactg gttcgatggg gcgaactacc ttagcgaact gacggaaaaa gagatcagcc
    30421 tgtggcgcaa tggcctaaac agcgttgagg atttacaggc gaaccatatc gacgaaacct
    30481 tcacctgggc gcaccgcatc agcaatcggg cggtagattt tctgcaacag cccgcgcgcg
    30541 ccgaggaacc cttcctgatg gtggtttcgt atgatgagcc gcatcacccg ttcacctgtc
    30601 cggtggagta tttagagaaa tacgctgatt tttactacga tctgggcgag aaagctcagg
    30661 atgacctggc gaacaaaccg gaacatcacc gcttatgggc gcaggcgatg ccatcgccag
    30721 tcggtgatga cgggctttat caccatccgc tctattttgc ctgtaatgac tttgttgatg
    30781 accaaatcgg acgggtcatc aatgccttaa cgccagagca acgtgaaaat acgtgggtca
    30841 tttatacttc cgatcacggc gaaatgatgg gcgcacataa gctgatcagt aaaggagcgg
    30901 cgatgtatga cgacatcacc cgtattccgc tgatcatccg ttcgccgcaa ggggagcggc
    30961 gacaggtcga tacgccagtc agtcatatcg atttactgcc gacaatgatg gcgctggcag
    31021 atattgaaaa accagagatt ctgccggggg aaaatatcct tgccgtgaaa gagccacgcg
    31081 gtgtaatggt ggaatttaac cgctacgaga ttgagcatga cagctttggc ggttttattc
    31141 cggtgcgttg ctgggtgacg gatgacttta aactggtact caacctcttc accagtgatg
    31201 aactttacga tcgccgtaat gacccaaatg aaatgcataa cctgatcgat gatatccgtt
    31261 ttgcagacgt tcgcagcaaa atgcatgacg ccttattgga ttacatggac aaaattcgcg
    31321 atccgttccg cagttaccaa tggagcctgc gtccgtggcg taaagatgca ctgccgcgct
    31381 ggatgggggc atttcgtcca cgcccacaag atggctattc gccggtggta cgtgactatg
    31441 acaccggcct accgacgcaa ggagtgaaag tggaagagaa aaaacagaag ttctgacgat
    31501 aatgcccgct gatgaacacc cggcgggcat tattcaggct gcttaaacca gtgaaccgcc
    31561 ggtcaggaag gaaaaaatca ggaatatcag caaaatgctg cacacgatgc cgataccaaa
    31621 aagcaaggta tggaaagcca gcgtcccgcc gccaaaaacc agcgcgccgc gaatgcccca
    31681 gcgattttcc ttacgcatac cgccaataaa tgcgcaaagt agcgcagcaa tggccagtgc
    31741 gataccgctt tgatcgagga ttttatcgac atctacgtcc tcttttttct ccacggtggt
    31801 aattttttcg ccttttattc cggcgagaag acctctttta atttcagccg ttttgtccgc
    31861 cacgatgctt tcaaacgttg gcggtggtgc ggaaaaaggg ccgaatgaaa agtggataat
    31921 ccccatcatt aaagcgatgg caccgaacag catgcccaat gaactgattt tattttgagc
    31981 tatgataccc atctgacctc cttgtagcgg atggcgaaag catatcagga aacgggcgga
    32041 cgaaattctt aaaaaaaaaa cgctaaaggt agagcgttag tgaaattgca aaggagcaac
    32101 aatgaaaatt tcccgccttg gagaagcgcc cgattaccgc ttctcgctgg ctaatgagcg
    32161 tacttttctg gcgtggatcc gtaccgcact aggatttctg gcggcggggg ttgggcttga
    32221 tcaacttgcg ccagatttcg ccacgcccgt cattcgtgaa ctgctggcgt tgttgttgtg
    32281 tctgttttcc ggcgggttag cgatgtatgg ctacctgcgc tggttacgca atgaaaaagc
    32341 gatgcgtctg aaagaggacc tgccctacac caacagctta ttaatcatta gcttaatatt
    32401 gatggtcgtg gcggtgatcg ttatgggact ggtgttgtat gccggatagc cgcaaagcca
    32461 gacgcattgc cgacccagga ctgcagccgg agcgcacatc actggcgtgg tttcgtacca
    32521 tgctgggcta cggcgcgttg atggcgttgg ctatcaaaca caactggcac caggcgggca
    32581 tgttattctg gatttcgatt ggcattctcg ccatcgtggc gctgatcctc tggcactaca
    32641 cccgtaatcg caatttaatg gatgtcacga atagcgattt ttcccaattt cacgtagtcc
    32701 gtgacaaatt tttgatctcc ctcgcggtgt tatctctcgc aatactgttt gctgtaacgc
    32761 atatacatca acttatcgta tttattgaga gagtcgcatg acaggaagtc aggtcattga
    32821 tgctgaagag gacaggcata aactggttgt agagtacaag gatgctctgc aacctgccga
    32881 tttttatcac aatttcaaac agcggggcat ccgctccgta cagctcattc cctatcttga
    32941 atttgatgac cggggcgatc tgacggccgc ttcggtgaca gcagagctgt gggggaaatt
    33001 tttaattgcc ctgttcgagt gttgggtgcg ggcagatatc agtcgtatct cgattgagct
    33061 ttttgacgcc accttacaaa aatggtgtgg cagcgaaaac ccgcaaccgc gccgtgactg
    33121 ccaggcgtgc gactggcatc gcctgtgccc gcacgctcgc caggagacgc cagacagcgt
    33181 gctttgtgcg ggctatcagg cgttttattc ttactccgca ccgcatatgc gggtgatgcg
    33241 tgatctgata aagcagcatc gttctcccat ggagctgatg acgatgctgc ggtgacgtgc
    33301 gcttaaacgg gctgcccctg atgcgacatc cgcgtcgcca gcggcagcca gcacagcacg
    33361 atcaacaatc ccattaaggt catcaacaac cccaggctgc cctgaccggt ttgcggcaac
    33421 atcgcagaga gcgacgccag cacgccggaa ccaatgtttt gcagaccgcc gaccagcgcg
    33481 ccagccgtgc ccgccaggaa ggggaacggc tccatcgcgc cgctggtcgc cagcggaaac
    33541 agcatcccgg caccgaaaaa gaacagcgcg gcgggaacga gcagcgtcca gacattcatc
    33601 acgccaaacc agtcggggat ccacatcagc aagccagcca gcaggcagca gataaccgac
    33661 tgccacatta acgtggagaa gcgtttattg ggacgtccgg caaaccatgc gccaaaaaac
    33721 gctgccggaa tcggcagaat aaacaaaata ctgaccgtca tactgctcag ccctaacacc
    33781 gcgcccatca gcacgccgga gcaggcttca aaggcggcaa tcccggccag accgccaatc
    33841 agcatcagca aataacagtt aaaaccgctg ttaccgaaaa gcgttttata actggtaagc
    33901 aggcgcgtgc gcggtgcatc gaccggacgc gtttccggca tccagcgggc catactgaag
    33961 gtcacaccag cacaaagaac caacaaaaag agataacagg cgcgccagtt ccacatcgta
    34021 tccagcagac cgccgattag cggtgcgagc aacggactga cgagaatccc catgtttaac
    34081 aggctgttag catggcgcaa ctgtgtccgt tcatataaat ctcgcggcaa agtacgcgcc
    34141 attacgccgc caacgccggt gcccatcccc tgcatcgcgc tggcggcaat caacaccgtc
    34201 aaactggagg tcgtgaccgc gaccagcgtt gccagcataa aaatggacat tccgacgagg
    34261 atcaccggtc ggcggcccac gcggtcggaa atcgggccat aaaacagctg tgagacaccg
    34321 taagtcagca gataagcgcc cattacgctc tgcaccgccc cttcacggac gttgagatcg
    34381 cgcgccatat cggcaatagc tggaatataa atggtttgcg ccatctgacc gacggccacg
    34441 agtaatacca acatcaataa caaattgacg tttctttgcc ttttcattat cacggatgct
    34501 tttataaaaa taattagaat aagtcactac gcattcccat aaatgcggga gaggaatcta
    34561 tcacattgat gaatgttagc cagatatacg ccaggaatgg ggaattgttt agcggcagaa
    34621 tatgtaaaca aaagcggcaa taaatgttgc cgggagggat tacgtgcgaa ctgtaaccct
    34681 ctccccaaag gggcgagggg gaaaccaccg ttccctttca gaaaatgagc taaacattgc
    34741 acgggacggt cccctcgccc ctttggggag agggctaggg tgaggggcac aggcagcgca
    34801 ggtgcaaaca ttctgctggt agaagaagcc cccaactcca acccgcacgc taaatataaa
    34861 aggggagcgg ttacccgctc ccctttggtg cgacttgaat ctgaattact tcaggtattt
    34921 cagaacagca tcaagcagtt gcagtgctgc aacaatgagt ttgaggataa gaatggcgat
    34981 atccaccagg ctcatacgcg tctcctgtgg ttcaggagca ctgccagctg acgtaccttt
    35041 ccgctgcctg ttgccagctg ttgtgcacgc ggtatcttaa ggagaaacgt tgtgcttgtt
    35101 gacataacac agtgtgctca cgcggggtta aggcgctgac ggcaccaccc gtttcagcca
    35161 ggacttagtg cgccgggtaa cgatgcggcc cccgcataac acattgcgta cagtgatatt
    35221 atagaagttt actgtataaa taaacagtaa tatttggaca aaacgcaaac tgtgatcgat
    35281 actcaactat gccctaaata cgtcaaaatt cgtgccgaaa ttgcgcgttc tgcgcggaac
    35341 acgtatactt tcagtgttga cataatacag tgtgctttgc ggttaccagc cgcaggcggc
    35401 tgatgaaacc tcgctccgcg gggttttttg ttatctgcaa ttcagtacaa aacgtgatca
    35461 acccctcaat tttctcttgc tgaaaaattt tccattgtct cccctgtaaa gctgtgcttg
    35521 tataaatatt gttaaacaca aaaccaacaa ggtccccaat gactacttcc atgttcaacg
    35581 caaaactact accaactgcg ctatccgccg cagtggtcgt cgtgcgtgtg gtggtggtcg
    35641 tcggcaatgc gccgtaggga ctggaacaac acacgattcc aaaaccccgc cggcgcaaac
    35701 cgggcggggt ttttcgttta agcacctccc ggaaagtcgg cccagaagaa aaggactgga
    35761 gcatggcaag ttcgggcaca acattgacgc gtaagcgctt taccggcgca gaatttatcg
    35821 ttcatttcct ggaacagcag ggcattaaga ttgtgacagg cattccgggc ggttctatcc
    35881 tgcctgttta cgatgcctta agccaaagca cgcaaatccg ccatattctg gcccgtcatg
    35941 aacagggcgc gggatttatc gctcagggga tggcgcgcac cgacggtaaa ccggcggtct
    36001 gtatggcctg tagcggaccg ggtgcgacta acctggtgac cgccattgcc gatgcgcggc
    36061 tggactccat cccgctgatt tgcatcaccg gtcaggttcc cgcctcgatg atcggcaccg
    36121 acgccttcca ggaagtggac acctacggca tctctatccc catcaccaaa cacaactatc
    36181 tggtcagaca tatcgaagaa ctcccgcagg tcatgagcga tgccttccgc attgcgcaat
    36241 caggccgccc aggcccggtg tggatagaca ttcctaagga tgtgcaaacg gcagtttttg
    36301 agattgaaac acagcccgct atggcagaaa aagccgccgc ccccgccttt agcgaagaaa
    36361 gcattcgtga cgcagcggcg atgattaacg ctgccaaacg cccggtgctt tatctgggcg
    36421 gcggtgtgat caatgcgccc gcacgggtgc gtgaactggc ggagaaagcg caactgccta
    36481 ccaccatgac tttaatggcg ctgggcatgt tgccaaaagc gcatccgttg tcgctgggta
    36541 tgctggggat gcacggcgtg cgcagcacca actatatttt gcaggaggcg gatttgttga
    36601 tagtgctcgg tgcgcgtttt gatgaccggg cgattggcaa aaccgagcag ttctgtccga
    36661 atgccaaaat tattcatgtc gatatcgatc gcgcagagct gggtaaaatc aagcagccgc
    36721 atgtggcgat tcaggcggat gttgatgacg tgctggcgca attgatcccg caggtggaag
    36781 cgcaaccgcg tgcagagtgg caccagttgg tagcggattt gcagcgcgaa ttcccgtgtc
    36841 caatcccgaa agcgtgcgat ccattaagcc attacggcct gatcaacgcc gttgccgcct
    36901 gtgtcgatga caatgcgatt atcaccaccg atgtgggtca gcatcagatg tggaccgcgc
    36961 aagcttatcc gctcaatcgc ccacgccagt ggctgacctc cggtgggctg ggcacgatgg
    37021 gttttggcct gcctgcggcg attggcgcgg cgctggcgaa cccggatcgc aaagtgttgt
    37081 gtttctccgg cgacggcagc ctgatgatga atattcagga gatggcgacc gccagtgaaa
    37141 atcagctgga tgtcaaaatc attctgatga acaacgaagc gctggggctg gtgcatcagc
    37201 aacagagtct gttctacgag caaggcgttt ttgccgccac ctatccggga aaaatcaact
    37261 ttatgcagat tgccgccgga ttcggcctcg aaacctgtga tttgaataac gaagccgatc
    37321 cgcaggctgc attgcaggaa atcatcaatc gccctggccc ggcgctgatc catgtgcgca
    37381 ttgatgccga agaaaaagtt tacccgatgg tgccgccagg tgcggcgaat actgaaatgg
    37441 tgggggaata agccatgcaa aacacaactc atgacaacgt gattctggag ctcaccgttc
    37501 gcaaccatcc gggcgtaatg acccacgttt gtggcctttt tgcccgccgc gcttttaacg
    37561 ttgaaggcat tctttgtctg ccgattcagg acagcgacaa aagccatatc tggctactgg
    37621 tcaatgacga ccagcgtctg gagcagatga taagccaaat cgataagctg gaagatgtgg
    37681 tgaaagtgca gcgtaatcag tccgatccga ctatgtttaa caagattgcg gtgttttttc
    37741 agtaaccgct caaggcttga acaacatcgc gcttatcgtt aaggtaagcg cgtttttttt
    37801 tacccgccag gacaagacca tgatcaccgt tgcccttata gacgatcacc tcatcgtccg
    37861 ctccggcttt gcgcagctgc tggggctgga acctgatttg caggtagttg ccgagtttgg
    37921 ttcggggcgc gaggcgctgg cggggctgcc ggggcgcggt gtgcaggtgt gtatttgcga
    37981 tatctccatg cccgatatct ccggtctgga actgctaagc cagctgccga aaggtatggc
    38041 gacaataatg ctctctgttc atgacagtcc ggcgctggtt gagcaggcgc ttaacgcggg
    38101 ggcacgcggc tttctctcca aacgctgtag ccctgacgaa ctgattgcgg cggtgcatac
    38161 ggttgccacg ggcggctgtt atctgacgcc ggatattgcc attaaactgg catccggtcg
    38221 tcaggacccg ctaaccaaac gcgaacggca ggtggcggaa aaactggcgc aaggaatggc
    38281 ggtgaaagag attgctgccg aactgggctt gtcgccgaaa acggtgcacg tccatcgcgc
    38341 caacctgatg gaaaaactgg gtgtcagtaa cgacgtagag ctggcgcgcc gcatgtttga
    38401 tggctggtga tgaagacgtt gttctcccgc ttaattaccg ttattgcctg cttttttatc
    38461 ttctctgccg catggttttg cctgtggagt atcagcctgc acctggttga gcgccctgat
    38521 atggcggtgc tgttatttcc gtttggtctg cgtctggggc taatgctgca atgcccgcgc
    38581 ggatactggc cggtgctgct gggcgcggag tggctgctga tttactggct aacgcaggcg
    38641 gtcggtttaa cccattttcc gttattgatg atcggtagtt tactgacgtt actgcccgtg
    38701 gcgctgatct cgcgctatcg ccatcagcgt gactggcgca ccttgctgtt acagggtgcg
    38761 gcgttaacgg cggcggcgtt gttgcagtcg ctgccctggc tttggcacgg caaagagtcg
    38821 tggaatgcgc tgttgctgac tttaactggc ggcctgacgc tggccccgat atgtctggtg
    38881 ttctggcact atctcgccaa taacacctgg ctgccgctcg gcccgtcact ggtttctcag
    38941 ccgatcaact ggcgcgggcg acatctggtc tggtacttgc tgctgtttgt tatcagtctc
    39001 tggctccagt tgggattgcc ggacgaactg tcgcgcttta cgccattttg tctggcgctg
    39061 ccgattatcg cgctggcctg gcactatggt tggcaagggg cgctgattgc gacgttgatg
    39121 aacgccatcg cgctgatcgc cagtcaaacc tggcgcgatc atccggtgga tttattgctc
    39181 tcgctgctgg tgcaaagtct gacagggttg ttgcttggcg ctggcatcca gcggttgcgt
    39241 gaacttaacc agtcgctgca aaaggaactg gcgcgcaatc agcatctggc tgaacggttg
    39301 ctggaaaccg aagagagcgt gcgccgtgat gtggcgcgtg agctgcatga tgatatcggt
    39361 cagaccatca cagccattcg tactcaggcg ggcattgttc agcggctggc ggcagataac
    39421 gccagcgtga agcagagcgg gcagctcatc gaacaactgt cgctgggcgt gtacgacgcg
    39481 gtgcgccgtt tattaggacg gttacgtccg cgccagttgg atgatctctc cctggagcag
    39541 gccatccgct cactgatgcg ggaaatggag ctggaagggc gcggcattgt cagccatctc
    39601 gaatggcgaa tcgatgaatc agcattaagt gaaaaccagc gcgtgacgct gtttcgtgtc
    39661 tgccaggaag ggctgaacaa cattgtgaaa catgctgatg ccagcgcggt cacgctgcaa
    39721 ggctggcagc aggatgaacg gttgatgctg gtgattgaag acgatggcag cggtttgccg
    39781 ccgggttccg ggcaacaagg ttttggcctc accggaatgc gcgagcgcgt aacggcgctg
    39841 ggcggcacat tacacatttc ctgtctgcac ggcacgcgtg tcagcgtttc tctacctcaa
    39901 cgctatgtct aaggttcgat gatgttgccg tttctgaaag cgcctgccga tgcgccatta
    39961 atgactgaca aacatgaaat tgatgcccgc tatcgctact ggcgtcggca tattctgctg
    40021 accatctggc tgggttacgc gctgttttac ttcacgcgta aaagttttaa cgccgccgta
    40081 ccagaaatcc ttgctaacgg cgtgctcagc cgtagcgata tcggcctgtt agcgaccctg
    40141 ttttacatta cctacggcgt gtcgaagttt gtctccggca ttgtcagcga tcgctcaaat
    40201 gcccgttatt ttatggggat agggcttatc gccacgggca tcatcaacat tctgtttggc
    40261 ttctcgacgt cgctatgggc gtttgccgtg ctctgggtgc tgaacgcctt tttccagggc
    40321 tggggttcac cggtgtgtgc gcgtctgtta acggcctggt attcacgtac cgagcgcggc
    40381 ggttggtggg cattatggaa cacggcgcat aacgtcggcg gcgcactcat tcccattgtg
    40441 atggcagcgg ctgcgctgca ttacggctgg cgtgccggga tgatgattgc tggttgtatg
    40501 gcgatagtcg tggggatttt tctctgctgg cggctacgcg atcgcccgca ggcgttaggt
    40561 ttaccggcgg tcggtgaatg gcgacacgac gcgctggaaa ttgctcaaca acaagaaggg
    40621 gcagggttga cgcgtaaaga gatcctcacc aaatatgtgt tgctgaatcc gtatatctgg
    40681 ctgctttcgt tttgctatgt gctggtctat gtggtccggg cggcgatcaa cgactggggc
    40741 aatttgtata tgtccgagac actgggcgtc gatctggtca cggcgaatac ggcagtgacg
    40801 atgtttgaac tgggcggatt tatcggtgcg ctggtagccg gttggggctc ggacaaattg
    40861 tttaacggca accgtgggcc gatgaatttg attttcgccg ccggaatttt gctttcagtc
    40921 ggctcgctgt ggctgatgcc atttgccagc tacgtgatgc aggcaacctg cttcttcacc
    40981 attggttttt ttgtctttgg cccgcagatg ttaatcggca tggcggcggc agagtgttcc
    41041 cacaaagagg cggcaggggc ggcgacgggg tttgtcggct tgtttgctta tctgggggcg
    41101 tcgcttgctg gttggccgct ggcgaaagta ctcgatacct ggcactggag cggatttttt
    41161 gtggttatct ctatcgccgc cgggatttcc gcactgctgt tactgccctt tttgaacgcc
    41221 caggcaccgc gcgaagcgtg atgcatctca ccttttcact tcatatccgg caaaactaag
    41281 aaattttcca ggttttgcct ggacgctatc tcaggcctga tttgctgctg atttttacaa
    41341 tgcatgcctc acgcaggtat tcatttcagg agtaacccat gctggctttc ttaaaccagg
    41401 ttcgcaagcc gaccctggac cttccgctcg aagtgcggcg caaaatgtgg ttcaaaccgt
    41461 tcatgcaatc ctacctggtg gtctttatcg gctacctgac gatgtacctg attcgcaaga
    41521 actttaacat cgcgcagaac gacatgatct cgacctacgg gttgagcatg acgcagctgg
    41581 ggatgatcgg cctgggtttc tccatcactt atggcgtggg taaaacgctg gtttcctact
    41641 acgccgacgg caaaaacacc aaacaattcc tgccgttcat gctgatcctg tctgccattt
    41701 gtatgctggg cttcagtgcc agtatgggca gcggctcggt tagcctgttc ctgatgattg
    41761 ccttctacgc cttaagcggc tttttccaga gtaccggcgg ttcgtgcagt tactccacca
    41821 tcaccaaatg gacgccgcgt cgtaaacgcg ggacattcct cggtttctgg aatatttctc
    41881 acaaccttgg cggtgcaggc gcagcaggtg tggcgctgtt cggggcaaat tacctgttcg
    41941 atggccatgt catcggcatg tttatcttcc cgtcgattat cgcgctgatt gtcggtttta
    42001 tcggcctgcg ttacggcagc gactccccgg aatcttatgg cctcggcaaa gctgaagaac
    42061 tgttcggcga ggagatcagc gaagaggaca aagagacaga atctaccgat atgaccaagt
    42121 ggcagatctt tgttgagtat gtgctgaaaa acaaagtgat ctggctgctg tgcttcgcca
    42181 acattttcct ctatgtggta cgtattggta tcgaccagtg gtcaaccgta tacgcgttcc
    42241 aggaactgaa actctctaaa gcggtggcga ttcagggctt tacgctgttt gaagctggtg
    42301 cgctggtcgg tacgctgctg tggggctggc tctctgacct ggcgaacggt cgccgtggcc
    42361 tggtggcctg catcgcgctg gcgctgatta tcgccacgct cggtgtgtat caacatgcca
    42421 gcaacgaata tatctatctg gcttctctct ttgcgttggg tttcctggtc tttggcccgc
    42481 aattgttgat tggtgtggct gctgttggct ttgtacctaa aaaagcgatt ggcgctgccg
    42541 atggtattaa aggcaccttt gcttacctga ttggtgacag ctttgccaag ttaggtctgg
    42601 gaatgattgc cgatgggacg ccggtattcg gccttaccgg ctgggcaggc accttcgccg
    42661 cacttgatat cgccgcgatt ggttgtatct gcctgatggc gatagtggcg gtgatggaag
    42721 aacgcaaaat ccgccgcgag aaaaaaattc agcagttgac agtggcataa acgtaactgg
    42781 tgacttttgc ccggcatgac gccgggcttt tttattattc cgtgacttcc agcgtagtga
    42841 aggcaaactt ctcgccatca aatagcccct gactggttag ttttagcgcg gggatcactg
    42901 gcagagaaag aaacgccatc tgaataaacg gctcatcggg taacggaccg cattcacggg
    42961 cggcggcttt caaggcgtca atttgttccg ccagcgactg cgccgtgtcg gtgctcatca
    43021 ggccggcaat gggcaacggc agatgacttt ggacctggcc gttacgtacc acgcacagcc
    43081 cgccgccatc ctgaatcacc tgattgaccg ccagcgccat ctcttcggca ctgcgaccga
    43141 tcaccacaat attatggctg tcatggctga ccgtcgcagc cagcgcacct tcattcagtc
    43201 caaagccgcc aagcaaacca caagccggag ccagccgttg cccgtaacgc tcaagtacgg
    43261 caataaagct cacatcatcg cgatcaaaac cattttcgct gtagacgctg gagtgggagt
    43321 gcgtaatcaa ttcgttatgg atgacgtcaa tgacccgata gcgttttccg ggcgtaaatt
    43381 gcagggcaaa gtcgctggcg gaaactggct ggcgggcaat ggtgttgcca tatggcggag
    43441 cggattgtgc cagtctcgcc gactcttccg cctgtaaggt ttgcgcatca atcggctcgc
    43501 ctttcaccag tacctgctgc accgtgacct tacgcgcatc gctcaacagg acgatatcgg
    43561 cctgcttgcc gggtgccagt aagccgaggt gattcagacc aaagtggcgc gccgtcgacc
    43621 agctggcgac gcgatatgcc acatgcagcg gcacattgtg ttgttcgatc aggcggcgaa
    43681 ttaaggcatc gatgtgtcct tcatgggcga tctcccacgg gttacggtca tcggtacaga
    43741 gcatgcattg cgggctgtta aattcgttga tcaacggtgc cagcgcgttg agattgcggg
    43801 cagcggaccc ttcgcggatc atcaacgaca tgccgagttg taatttccgg cgtccttctt
    43861 ccagctgata actttcgtgg cagttttcaa tacccgcagt aatataggcg ttaagttctt
    43921 taccacccaa acccgggcag tgaccgtcca gcgtcaggtg gcgaaatgca tccagtttat
    43981 cgagcagcgc attctgcccg ctaattacgc cagggtagtc catcatttct gcaaggccgg
    44041 taacctgcgg atggtcccgc caggcgagca tctgttcaag ggtaaaactg gcaccgttaa
    44101 catcgcagcc ttccagggcg ggtacgcaag agctgacctg taagtactgg ttttgccttg
    44161 cctgttcggc acagcgggca aaccaggcga atccggcttc tcccatcacg ttgacgattt
    44221 catgagggtc gcaaataacg gtcgtcaggc cgcgcggcag ggtagcggtt tcaaaagtga
    44281 ccggcgtcat catgctggat tcaatatgca ggtgagcatc aataaaccct ggcaccgccg
    44341 ttgcgccgcg agcatcaatc cgctgcaaag ccggagcatc agcgtattct gcgccaacac
    44401 cggcaatgta acgtccttta atcacaattg ggccggaaat ttctccgcca ttgatcaggt
    44461 cgagaataga gacattatca ataatataat cggcaacagc gtcgccacgg gaaacggcta
    44521 acaattcctg gtattcagcc cggct
//
LOCUS       DAPHDW010000024        42763 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.024, whole genome shotgun sequence.
ACCESSION   DAPHDW010000024 DAPHDW010000000
VERSION     DAPHDW010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 42763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42763
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.024"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1240)
                     /locus_tag="RH057_001260"
     CDS             complement(<1..1240)
                     /locus_tag="RH057_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3127664.1"
                     /translation="MALTEVKVRNAKPTEKPVKLTDGDGMHLLVHPTGSKYWRLQYRF
                     SGKQKMLALGVYPEVSLAEARRRREEARQLIANNVDPGEKRKTEKIEEKGLLIFETVA
                     RDWHSSNRTWSDSHRTTVLNSLITHVFPVIGKRNISELKTRDLLVPLKKAEETGHLEL
                     AARLQQRITAIMRYAVHNALIDQNPAYDLAGAVTTAKSNHRPALPLERLPELLKRIDA
                     YKGKGITQIAVQLTLLTFIRSSELRFARWSEVNFKNSLWTIPAERRELEGVKYSNRGS
                     KMKTPHLVPLSKQAVVLFKRLQAISGEKDLMFIGFSRDDKPISENTVNKALRTMGYDT
                     KAEVCGHGFRTMACSALIESGLWSRDAVERQMSHQERNSVRAAYIHKAEHLEERKLML
                     QWWADYLNANRKEHITPFEFA"
     gene            complement(1505..1705)
                     /gene="torI"
                     /locus_tag="RH057_001261"
     CDS             complement(1505..1705)
                     /gene="torI"
                     /locus_tag="RH057_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator inhibitor TorI"
                     /protein_id="HDV3127665.1"
                     /translation="MQHELQPDSLVDLKFIMADTGFGKTFIYDRIKSGDLPKAKVIHG
                     RARWLYRDHCEFKNKLLSRANG"
     gene            complement(1763..1933)
                     /locus_tag="RH057_001262"
     CDS             complement(1763..1933)
                     /locus_tag="RH057_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127666.1"
                     /translation="MHFRVTGEWRNGEPFNRVIEAENINDCYDHWMIWAQIAHADVTN
                     IRIEELKEHQAA"
     gene            complement(2006..2290)
                     /locus_tag="RH057_001263"
     CDS             complement(2006..2290)
                     /locus_tag="RH057_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HDV3127667.1"
                     /translation="MANLQLAVKGEYFDAMIRGEKTEEYRLCNDYWNKRIMLREYDRL
                     IITKGYPKRDDSSRRIDVPYGGYEVKTITHPHFGDKPVKVFAIKVNISTE"
     gene            complement(2290..2550)
                     /locus_tag="RH057_001264"
     CDS             complement(2290..2550)
                     /locus_tag="RH057_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="eaa protein"
                     /protein_id="HDV3127668.1"
                     /translation="MIDVKEKVLQVMRSRAALQEKALGGEYPFTIATWNLRLAMEKEF
                     PDEEWRSADLRKILMELAKDGAVSKDTYASRIGQAVWRLEVR"
     gene            complement(2547..2696)
                     /locus_tag="RH057_001265"
                     /pseudo
     CDS             complement(2547..2696)
                     /locus_tag="RH057_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162258.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
     gene            complement(2697..2984)
                     /locus_tag="RH057_001266"
                     /pseudo
     CDS             complement(2697..2984)
                     /locus_tag="RH057_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450597.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(2981..3148)
                     /locus_tag="RH057_001267"
     CDS             complement(2981..3148)
                     /locus_tag="RH057_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2737 family protein"
                     /protein_id="HDV3127669.1"
                     /translation="MRGLAYNPDILPAEMIIRQRVKPMPSREELLKRNSFPSVNENKY
                     LNAMWRKGGSQ"
     gene            complement(3159..3452)
                     /locus_tag="RH057_001268"
     CDS             complement(3159..3452)
                     /locus_tag="RH057_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2856 family protein"
                     /protein_id="HDV3127670.1"
                     /translation="MPAPLYGADDPRRCSGNSVSEVLDKFRKNYDRIMSLPQETKEEK
                     EFRHCIWLAEKEERERIYQTSIRPFRKATYTHFPEIDPRLRNYRSRYGAISND"
     gene            complement(3476..3859)
                     /locus_tag="RH057_001269"
     CDS             complement(3476..3859)
                     /locus_tag="RH057_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000951334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127671.1"
                     /translation="MAHSITVRLNKPAREFQAGENIGFNIRAGVQYYDRQTKKKEWTN
                     YSAVIFAKPGAQADYYRSVLVEGGIVEITGENIRVDVYQGQNGQSITLELLNAKIGFA
                     TSGNSPQQQSSNQQNTPVYDDSIPF"
     gene            complement(3859..4464)
                     /locus_tag="RH057_001270"
     CDS             complement(3859..4464)
                     /locus_tag="RH057_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ERF family protein"
                     /protein_id="HDV3127672.1"
                     /translation="MSKEFYARLAAIQENLNAPKNQYNSFGKYKYRSCEDILEGVKPL
                     LNGLFLSISDEVVLIGDRYYVKATATITDGENSHTATALAREEESKKGMDSAQVTGAT
                     SSYARKYCLNGLFGIDDAKDADTDEHKHQQNAAAKQSKPSPTPEQVLKAFTDAAMQKN
                     TVEELKQAFAKAWKMLEGTPEQHKAQDVYNIRRDELEGAAA"
     gene            complement(4475..4645)
                     /locus_tag="RH057_001271"
     CDS             complement(4475..4645)
                     /locus_tag="RH057_001271"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127673.1"
                     /translation="MSLATTVKESKLQRRMYTQQALMYRQKGDREGVRVFLNAAKTEV
                     LNQRYFLGPCPF"
     gene            complement(4721..4873)
                     /gene="kil"
                     /locus_tag="RH057_001272"
     CDS             complement(4721..4873)
                     /gene="kil"
                     /locus_tag="RH057_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitory peptide Kil"
                     /protein_id="HDV3127674.1"
                     /translation="MRNEIAINHQMLRAAQNKAVIARFIGDSKMWLEANKAMKSAINL
                     PLYRRK"
     gene            complement(4858..4992)
                     /locus_tag="RH057_001273"
     CDS             complement(4858..4992)
                     /locus_tag="RH057_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016248931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127675.1"
                     /translation="MMHFQLAGSGVMSAFYPHESELSRRVKQLIRAAKKQLEALCAMK
                     "
     gene            complement(5227..5409)
                     /locus_tag="RH057_001274"
     CDS             complement(5227..5409)
                     /locus_tag="RH057_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127676.1"
                     /translation="MGKDENIIVEDVFHQDYGPEDGIPHHWCCKFYRDGFADYEYFST
                     KDEAYDFAFKNGYKPF"
     gene            complement(5611..5781)
                     /locus_tag="RH057_001275"
     CDS             complement(5611..5781)
                     /locus_tag="RH057_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127677.1"
                     /translation="MQWKCLPAKRSYLRGRIPEIKTTDGEVSGESDIHSIMLEKSAKA
                     ESKAPQASHKGR"
     gene            complement(5700..5972)
                     /locus_tag="RH057_001276"
     CDS             complement(5700..5972)
                     /locus_tag="RH057_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127678.1"
                     /translation="MTVVITYLADDNARNRRRARRQAQREQAMQEQRLARKIARKLSG
                     CVRADKAASLGSLRCKKADECSGSVCLPNVAIYAAGYRKSKQLTAR"
     gene            complement(6678..7046)
                     /locus_tag="RH057_001277"
     CDS             complement(6678..7046)
                     /locus_tag="RH057_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127679.1"
                     /translation="MVGTAIASFLGMLAISTIYGLAHAFIAKSLSEKISQAWAHRSAR
                     FMILVIIAIQGISAFILYGSSLYLLYQGATFTPYTSDYGTLYDGSEDISMAWIVFGLS
                     MAVSVVADIIKVILVLTFAD"
     gene            complement(7065..7781)
                     /locus_tag="RH057_001278"
     CDS             complement(7065..7781)
                     /locus_tag="RH057_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3127680.1"
                     /translation="MENKKSLTTEQLEDAKRLKALYESKKKELGITQYSIADELGITQ
                     GAVGHYLNGRNALNVEVASGFARLLQVSIADFSQSIAAKVAEQAESLKSDANVRYAGE
                     YRAGKRYPVLSSIQAGSWCEACEPYTIKDIDVWLESDAHIQGNAFWLKVEGDSMTAPV
                     GLSIPEGTFVLFDTGREAINGSLVIAKLSDSNEATFKKLIIDGGNKYLKGLNPAWPLV
                     PINGNCKIIGVAIETKLRLV"
     gene            7888..8082
                     /locus_tag="RH057_001279"
     CDS             7888..8082
                     /locus_tag="RH057_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HDV3127681.1"
                     /translation="MIRMTLADYAKIHGQAKAASDFGVIQCAISKAILAGRNIMVTVK
                     PDGSVIGEEVRPFPSNKKNK"
     gene            8191..8469
                     /locus_tag="RH057_001280"
     CDS             8191..8469
                     /locus_tag="RH057_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001375758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lambda phage CII family protein"
                     /protein_id="HDV3127682.1"
                     /translation="MQLTSTRKKANAITSNILNRIAVRGQRKVADALGINESQISRWK
                     DSFIPKMAMLLAVLEWGVEDEELAELAKKVAMVLTKEKPQDCGNSFEA"
     gene            8504..8665
                     /locus_tag="RH057_001281"
     CDS             8504..8665
                     /locus_tag="RH057_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127683.1"
                     /translation="MTKRRKKYQEKEEIRHPDSPEGLVVAAANNRAFAERLVGVYRLA
                     KAGVKHGRR"
     gene            8652..9542
                     /locus_tag="RH057_001282"
     CDS             8652..9542
                     /locus_tag="RH057_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein"
                     /protein_id="HDV3127684.1"
                     /translation="MGVVKLADYRHNPVQHQEASSMGYVSIHRQFMDSRLYKDSQAVH
                     LWLHLILKANHESTVVNTDIGPITVDRGQMITGRPSLVRETFIPDNKVRSLLRTFESK
                     GMLNICSMGKKFSLFTIVKYDDFQAKNCPTVVQRLSNANSSNGAALSGDYPTVVQRLS
                     INNNINNISNTDVLESATADKKSDKKKPSVSCQDVVDAYHEILPEAPRIRALNDKRKN
                     QIRTFWRKAGVITRQLDGHGFTMQDWRNYLSYVGENCRWMFEERPNHQRGTVWHKKGF
                     DFLLNDNTYLKVREGEHDDR"
     gene            9532..10968
                     /locus_tag="RH057_001283"
     CDS             9532..10968
                     /locus_tag="RH057_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV3127685.1"
                     /translation="MTDNFYAPPHSIEAEQAVIGGLLLDDDSSERVQKVLAMLKPDSF
                     YSRPHKIIFEEITRMHREQKPVDGLTLFDELERKSLTASVGGFAYIAEIAKNTPSAAN
                     IVAYAMQVRETAMERYAINRMTEATELLYSRNGMTATQKYEAIQAIFTQLTDHAKTGS
                     RRGLRSFGEVMEDWVSDLEKRFDPSGEQRGMSTGIPSLDRMLSPKGLVKGSLFVIGAR
                     PKMGKTTLYSQMAINCAVHEKKPALMFSLEMPGDQILEKLVGQKSGVNPNIFYLPATN
                     DADDGYQGDYDGDFNRAIETANRLSEIDLLYIDDTPGLSLAQIVSESRRIKREKGCVG
                     MILVDYLTLMTAEKADRNDLAYGMITKGLKNLAKELDCVVVLLTQLNRALESRTNKRP
                     LPSDSRDTGQIEQDCDYWVGIHREGAFDDSVPPGETELILRLNRHGNTGTVYCIQANG
                     AIYDTDQQSAEMRRREREEPQSKKKGGF"
     gene            11246..11833
                     /locus_tag="RH057_001284"
     CDS             11246..11833
                     /locus_tag="RH057_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017441424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV3127686.1"
                     /translation="MKKLTFEIRSPAHQQNAIHAVQQILPDPTKPINQITQQRLKEVL
                     LYDELTGKFTWKVKKCQRMNAGDIAGHKSSEGYWVIKVDGKLYKAHRLAWLYMNGSLP
                     KSDIDHINLVRDDNRIANLRLATRSQNIQNVNKKANNKSGYKGVSWDKKSRKWRAQIV
                     INKRKVNLGFYDDPKEAHKVYANKADECFGEFARY"
     gene            11835..12263
                     /locus_tag="RH057_001285"
     CDS             11835..12263
                     /locus_tag="RH057_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinB"
                     /protein_id="HDV3127687.1"
                     /translation="MKCVKYHLTNESIRYNAIQYLRTCDLDIIVEFKQRNRSLDQNRK
                     LWACLGDVSRQVEWHGRWLDAESWKCVFTAALKQQDVVPNLAGNGFVVIGQSTSRMRV
                     GEFAELLELIQAFGTERGVKWSDEARLALEWKARWGDRAA"
     gene            12260..12787
                     /locus_tag="RH057_001286"
     CDS             12260..12787
                     /locus_tag="RH057_001286"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage N-6-adenine-methyltransferase"
                     /protein_id="HDV3127688.1"
                     /translation="MTIKSNTPAHDKDCWQTPLWLFDALDIEFGFWLDSAASDKNALC
                     AHWLTEADDALNSEWVSHGAIWNNPPYSNIRPWVEKAAEQCIQQRQTVVMLVPEDMSV
                     GWFSKALESVDEVRIITDGRINFIEPSTGLEKKGNSKGSMLLIWRPFISPRRMFTTVS
                     KAALMAIGQGVRRAA"
     gene            12784..12966
                     /locus_tag="RH057_001287"
     CDS             12784..12966
                     /locus_tag="RH057_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016063117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NinE family protein"
                     /protein_id="HDV3127689.1"
                     /translation="MRRQRRSITDIICENCKYLPTKRSRNKRKPIPKESDVKTFNYTA
                     HLWDIRWLRERARKTR"
     gene            12963..13133
                     /locus_tag="RH057_001288"
     CDS             12963..13133
                     /locus_tag="RH057_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninF"
                     /protein_id="HDV3127690.1"
                     /translation="MIDPNRSYEQESVERALTCANCSQKLHVLEVHVCEHCCAELMSD
                     PNSSMYEEEDDG"
     gene            13126..13737
                     /locus_tag="RH057_001289"
     CDS             13126..13737
                     /locus_tag="RH057_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein NinG"
                     /protein_id="HDV3127691.1"
                     /translation="MAKPARRRCKNDECREWFHPAFANQWWCSPECGTKIALERRSKE
                     REKAEKAAEKKRRREEQKQKDKLKIRKLALKPRSYWIKQAQQAVNVFIRERDRDLPCI
                     SCGTLTSAQWDAGHYRTTAAAPQLRFDERNIHKQCVVCNQHKSGNLVPYRVELINRIG
                     QEAVDEIESNHNRHRWTVEECKAIKAEYQQKLKDLRNSRSEAA"
     gene            13734..13940
                     /locus_tag="RH057_001290"
     CDS             13734..13940
                     /locus_tag="RH057_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage NinH family protein"
                     /protein_id="HDV3127692.1"
                     /translation="MTFSVKTIPDMLVEAYGNQTEVARRLKCSRGTVRKYVDDKDGKM
                     HAIVNDVLMVHRGWSERDALLRKN"
     gene            13918..14589
                     /locus_tag="RH057_001291"
     CDS             13918..14589
                     /locus_tag="RH057_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001554887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HDV3127693.1"
                     /translation="MRYYERIDGSKYRNIWVVGDLHGCYTNLMNKLDAIGFDTQKDLL
                     ISVGDLVDRGAENVECLELITFPWFRAVRGNHEQMMIDGLSERGNVNHWLLNGGGWFF
                     NLDYDKEILAKALAHKADELPLIIELVSKDKKYVICHADYPCDEYEFGKPVDHQQLIW
                     NRERISNSQDGIVKEIKGADTFIFGHTPAVKPLKFANQMYIDTGAVFCGNLTLIQVQG
                     EGAWA"
     gene            14580..15098
                     /locus_tag="RH057_001292"
     CDS             14580..15098
                     /locus_tag="RH057_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HDV3127694.1"
                     /translation="MGIRELNLTKEQHEWLNGWLELWGAWVYSGRLEKRMSSVIAKFM
                     ESVEPGRVMTRPMCNDDDGMLISQVVDSVMYIDKKAFGILLSYYAHGSSKRAIASYYH
                     ATAKPRKMCGRGGEGWRKPSMATCRNEIDDILKASLFVLYQPMQNAFKMRKRVEKVKH
                     VAVKSLDMQLAI"
     gene            15162..15236
                     /locus_tag="RH057_001293"
     tRNA            15162..15236
                     /locus_tag="RH057_001293"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15195..15197,aa:Asn,seq:gtt)
     gene            15242..15317
                     /locus_tag="RH057_001294"
     tRNA            15242..15317
                     /locus_tag="RH057_001294"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15275..15277,aa:Thr,seq:tgt)
     gene            15560..15883
                     /locus_tag="RH057_001295"
     CDS             15560..15883
                     /locus_tag="RH057_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin, lambda family"
                     /protein_id="HDV3127695.1"
                     /translation="MKMPEKHDLLAAILAAKEQGIGAILAFAMAYLRGRYNGGAFTKT
                     VIDATMCAIIAWFIRDLLDFAGLSSNLAYITSVFIGYIGTDSIGSLIKRFAAKKAGVE
                     DGGNQ"
     gene            15867..16343
                     /locus_tag="RH057_001296"
     CDS             15867..16343
                     /locus_tag="RH057_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family protein"
                     /protein_id="HDV3127696.1"
                     /translation="MVEINNQRKAFLDMLAWSEGTDNGRQKTRNHGYDVIVGGELFTD
                     YSDHPRKLVTLNPKLKSTAAGRYQLLSRWWDAYRKQLGLKDFSPKSQDAVALQQIKER
                     GALPMIDRGDIRQAIDRCSNIWASLPGAGYGQYEHKIGDLIARFKKAGGVVNEAEI"
     gene            16327..16719
                     /locus_tag="RH057_001297"
     CDS             16327..16719
                     /locus_tag="RH057_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2570 domain-containing protein"
                     /protein_id="HDV3127697.1"
                     /translation="MKLRYRLVIVAFVVTVIGSFIWSAGHYYSKYQHEKERADEAVRN
                     AESATAITRNVLQSLQIINTVIEANQHAKEQIALDASGASADIRVAVANDDCTNRPVP
                     AGAVKRLHEYANGLRAGSGSSITSQPDG"
     gene            16863..17048
                     /locus_tag="RH057_001298"
     CDS             16863..17048
                     /locus_tag="RH057_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006120181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127698.1"
                     /translation="MARLNVEVTPPDSEALNGIFAEIERKYARQPLTPKVIDEMQREA
                     TRLVRRMITTKVTFVRD"
     gene            17105..17725
                     /locus_tag="RH057_001299"
     CDS             17105..17725
                     /locus_tag="RH057_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127699.1"
                     /translation="MNRPHPPSHFTMPPDPKPYISIMPASDVGEWLNQHILSDEGDLY
                     NPDHQHLLEADLCFLWASNAFEKKGRSVLGQAEEVAMRAGGWQKARMEQQMYEWFGRV
                     PQFIITLAADYCSQCSDLEFCALIEHELYHICQATDEFGAPKFTQEGQPKLKLRGHDV
                     EEFVGVVRRYGASRDVQEMIDVANQPAEVAHLDIARACGTCMLRLA"
     gene            17757..18230
                     /locus_tag="RH057_001300"
     CDS             17757..18230
                     /locus_tag="RH057_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2280 domain-containing protein"
                     /protein_id="HDV3127700.1"
                     /translation="MAALKSDVKAFIIQSLACYDTPSQVVEAVQKEFGIKITRQQAES
                     HDPTKASGKTLAKKWIEMFHATRERFLTETSDIPIANKSYRLRVLDRMATKTEGMKNF
                     SLTAQLIEQAAKEVGDAYTNKLKVESTGKDGGPIKTETTNLTADQAAEIYRKMMG"
     gene            18233..19855
                     /locus_tag="RH057_001301"
     CDS             18233..19855
                     /locus_tag="RH057_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerL protein"
                     /protein_id="HDV3127701.1"
                     /translation="MPLPFEFDFRNPDYQMVFEWRMERLQRIRQNPEMLPALKQFYRT
                     NPAQFIIDWGMTTDPRNIDYGLPVTIPFLLFPKQEEWIHWIMERRERLENGITEKSRE
                     MGLSWTAIGLACSLCLFNKEMVIGFGSRKEEYVDSTGDPKALFWKARKFVETLPIEFR
                     GSWDEKKHAPYMRVEFPDTGAVIKGEAGDNIGRGDRTTLYLVDEAAFLQRPLLIDAAL
                     SQTTRCRIDLSSVNGMANPFAQKRHGGKIPVFTFHWRDDPRKDEEWYRRECEKIDNPV
                     VVAQELDLNYSASAEGVLIPSEWVQAAVDAHIKLGIQPTGKRLGAMDVADEGRDKNAF
                     STRHGFLLENVREWSGVGSDIYQSVEKVFGFCEQDNLEEFRFDEDGLGAGVRGDARAI
                     NEQRNAARLPSILATPFRGSGAVFDPDDEAVRGDNGQAARLNKDFFANAKAQSWWRLR
                     KLFQNTWRAVVEGMAYNPDEIISISSSMALKDKLIIELSQPTYSINGVGKIVIDKQPD
                     GTRSPNLADSVMINYAPMNSALNIWELLGRQA"
     gene            19855..21321
                     /locus_tag="RH057_001302"
     CDS             19855..21321
                     /locus_tag="RH057_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HDV3127702.1"
                     /translation="MARNKQALRRTAQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGTLEAQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRLETIGEGKFKGILPLDRWMINPVLTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRVSSLQERRLRMPMRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVAVNTTNAISTALGDGLMTRKAAMTDLRENSDVTGIGASITDEDIENAEDEAPPGIG
                     ELGDKPPESPGGNPISNEPTADSAGGRGYRKWSLRWFK"
     gene            21209..21946
                     /locus_tag="RH057_001303"
     CDS             21209..21946
                     /locus_tag="RH057_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000184960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HDV3127703.1"
                     /translation="MATNRQSRQAEIRYRTSLRQIARAVGDIVNGRYDGSNDSVTEIM
                     DALERYSEIITPWATKVAENFTADIARQNEKQWRQHSRNISAELRNMVDRAPVGQVMK
                     SIVAEQIKYIKSLPLEAADRVYDIQNKAIEAVVSGGRAEPFAKEIAASGDVSRSRANL
                     IARTELGRATGALDQARALSIGSNGYIWRTAEDGDVRHSHREMEGKFVEWGKPPTLDG
                     MTGHAGELPNCRCYKEIVFPNPHSYLA"
     gene            21961..23181
                     /locus_tag="RH057_001304"
     CDS             21961..23181
                     /locus_tag="RH057_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HDV3127704.1"
                     /translation="MKYFFNTRLGETRYQLADGSLLCKDVPIGRTGKQLYGAADLPNL
                     KPDKLGEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKELAVGH
                     LQNVRRGTGEQSDLMLADLIVKDENAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGGPTGDSDDDLKTLLKALLAKLEGNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEIAKGRNTRATTGDAQRQNMGMTSIAS
                     LNKQNAEFWSNRKG"
     gene            23186..23689
                     /locus_tag="RH057_001305"
     CDS             23186..23689
                     /locus_tag="RH057_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127705.1"
                     /translation="MTAYLYRMPVGIAGAISRPQDLTVEPVVLKSDNAFAAYGLAGKY
                     DDDGFFVPLADGDTADKVKGIYVRPYPTTSQPDMVRQVGSGKNFPGDAMKRGYVTVNL
                     GSDFDASTIKKGDPVYVVVSTDESIKVPLGGFMATSVSGKNVVLTNAEFTGAGDANGN
                     AEISWKI"
     gene            23701..24642
                     /locus_tag="RH057_001306"
     CDS             23701..24642
                     /locus_tag="RH057_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HDV3127706.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWTRDIQLRE
                     DVSIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLNGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTYLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRMVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(24653..24871)
                     /locus_tag="RH057_001307"
                     /pseudo
     CDS             complement(24653..24871)
                     /locus_tag="RH057_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24870..25277
                     /locus_tag="RH057_001308"
     CDS             24870..25277
                     /locus_tag="RH057_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4054 domain-containing protein"
                     /protein_id="HDV3127707.1"
                     /translation="MPKNQSLPAVSDFRRDFPQFADPAKYPEAQIQFRLNLADELLSE
                     NVTGKKLFPYFAGLFVAHYMTLWAADSRAMLAGGPGGSTNGVQSSKSVDKVSVSYDTS
                     ATLNHDAGFWNNTRYGAEFYQLITMFGAGGRQL"
     gene            25274..25828
                     /locus_tag="RH057_001309"
     CDS             25274..25828
                     /locus_tag="RH057_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127708.1"
                     /translation="MSFKSGVTTRVDNAQAILDALKSISKKEVLVGIPEADSEREDVP
                     FGNAGIGYLNEYGSPAQNIPPRPHLIPGVKSVEEQTLPQLKTAAQAALDGNAAGAERA
                     LNRAGTLAANGVRRYMTITGFTPLADSTVEARARRGRKGAKAELARRAAGESPGTDLV
                     KPLIDTGQYRRAITHVVRDKDADS"
     gene            25815..26204
                     /locus_tag="RH057_001310"
     CDS             25815..26204
                     /locus_tag="RH057_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001687820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor"
                     /protein_id="HDV3127709.1"
                     /translation="MPTLDVTDVLFDPDFCDFNLWVTRRVQTVDDDGIGSDSEVKTQF
                     AGVVTVDRSLENRRMQSGQVISGAILIVTTERLTQGQTGRDADIVTYQGRDYRVTFVD
                     PYTAYGSGFVQAHCELLPFDGGIPVEQ"
     gene            26179..26742
                     /locus_tag="RH057_001311"
     CDS             26179..26742
                     /locus_tag="RH057_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127710.1"
                     /translation="MGEFRLSNNTSTERGWLIPTSGDPDYDEALDRLLSQWMRNVSGL
                     SAGMVRPRWQKEQPPLLPVETNWCAFGVIGWSGDDSPAFTRQTDDGSQLCRHETIECM
                     ASFYGPAGMVYASRFRDGISVPQNNAALNALGLSLGDYTGLTPFPELINQQWVRRYDM
                     KVRLRRKVVREYGIKSLVEAPVIFFGD"
     gene            26746..27891
                     /locus_tag="RH057_001312"
     CDS             26746..27891
                     /locus_tag="RH057_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3383 domain-containing protein"
                     /protein_id="HDV3127711.1"
                     /translation="MAQGLPVSNVVNVDVIMSPRAASGRNFGALLILGPSTIIPVSER
                     IRRYSAAEDIGKDFGVESPEYKAAQVFFSQSPKPQEVFVGRWVKTKGDSEQATPETLE
                     QAVNAMLDYTSWYGLGIADDADIPDADWLKVAAAIESSSVSRILAITTSDEKCLQTAS
                     SDDLASKLKTAGYSRSFIQYSSGNKYAALSAFGRAFTVNFNGSNTAITLKFKQEPGVG
                     YETLTVSQASALDAKNCNVFVYYQNDTAILQQGVMANGDFFDERHGLDWLQNYVQTNL
                     YNLLYTSTTKVPQTEAGITRLLSNVEKSLDQAVQNGLIAPGVWNGGDLGQLSSGDTLP
                     KGYYVYAQPLDEQAQSEREARKAPVIQAAIKLAGAVHYADVQINVVR"
     gene            27902..28342
                     /locus_tag="RH057_001313"
     CDS             27902..28342
                     /locus_tag="RH057_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3277 family protein"
                     /protein_id="HDV3127712.1"
                     /translation="MSTYSFMDVTATLTGPTGSIDLGYGSASSEEGIVVAMGGPKNTM
                     TIGSDGEVMHSLHADKSGTITVNLLKTSPTNKKLSLAYNAQSQSSATWGNNVIVIRNK
                     VSGDIITARSVAFQKQPDNANAKTGNTMPWVFDCGKIDQVLGEF"
     gene            28346..28798
                     /locus_tag="RH057_001314"
     CDS             28346..28798
                     /locus_tag="RH057_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001703548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127713.1"
                     /translation="MEFEIKGVKYRAAKLSVFDQLKVTRKLLPVLAGMMSDFGSIRSR
                     LPADGKIDTVKFEQLKPVFETMLPRIAEELSSLTEDDTSAIIHPCLAVVSRRHMDGWV
                     PVFTQGELMFDDIDLLVMLHLVARVVADSLGNFLPTPLTSTTQSLQQG"
     gene            28976..30964
                     /locus_tag="RH057_001315"
     CDS             28976..30964
                     /locus_tag="RH057_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase"
                     /protein_id="HDV3127714.1"
                     /translation="MNAETIKDFLVSLGFSVDDAGAKKFGSVLAGTTANVIKMGLAVE
                     GAALSVVAFTAKIASGLDNLYRASQRTGATVQGIQSIGYAVSQVGGSVDAARTSLESL
                     SRFVRNNPGAEGFLNRLGVQTRDASGNMRDMAAIFTGVGQKLSGMPYYRANQYAQMLG
                     IDENTLMAMRRGVGDFSGQYSAMAKAIGFNADEAARSSNKFMTSLREFGAMAGMARDK
                     IGSNLAGGLAGSLDTLRRHILDNFPRIEQTLTKAIKGILAIGDIIGRLFFRLIEGTSS
                     LITWWQSLDKQTRELISLFGALTIALRILNSTFWMSPIGLITALAAGIALLWEDYQTW
                     KEGGDSLIDWGKWKPEVDAALKMVRDLKGSVNELAKALAKLLNIDPKSWSLKWDFSNF
                     IDQMGEFSKMLNMIADLLNAIKDGRWADAVIIGKQILNQGSENPSAMPMVTDSANSTA
                     DWIKEHWGFDPRSVGRTVRGWFGDDEPEQYAQATKRGERNNNPGNLNFAGQAGASLER
                     PGGRFARFETAFDGLRALARQLMLYAGRGINSVEKIISTWAPASDNNNTTAYIRAVSQ
                     RLGVDPRAALNMSDPQTMSALMSSIIQHENGRNIYSRELINKAAVAGISGKMTEVNQQ
                     NTYHIYGGGDPHAVGNEVARRQQSANAQVMRSNQVRVG"
     gene            30964..31551
                     /locus_tag="RH057_001316"
     CDS             30964..31551
                     /locus_tag="RH057_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127715.1"
                     /translation="MDILSTLFHQQSRKIGMIVPSVVISEKHTDMLEITEHPVEVGAA
                     VADHAYKKPSEVVMEVGFAGGGALLDFASNLTATSLLGLSPQQTYQELLDLQESRIPF
                     DVVTGKRLYSNMLIRALEVTTDKTTENVLSAVLTLREVIISRTQQITVADKTNMKEGA
                     STSAVQNSGNKTTKPPDTSLLKSITGNVASLLGGG"
     gene            31551..31853
                     /locus_tag="RH057_001317"
     CDS             31551..31853
                     /locus_tag="RH057_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127716.1"
                     /translation="MTIQEIPLTADNQQFSIVLGGVTWRISIIWRDPYWIMDLQNDRG
                     EPVISGIPLVTGADLLAQYACMGLGFKLLVVCDDNTQDYPTKTDLGGRSHLLVSTE"
     gene            31856..32914
                     /locus_tag="RH057_001318"
     CDS             31856..32914
                     /locus_tag="RH057_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001482687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127717.1"
                     /translation="MSQNWMRHFELQLVDGNGKGIELSDFKVTFTIDWFNISSASRVG
                     TIKIYNLSADTVNRITGQEFSKVRLIAGYDGIAPEVAASDVGTVREVDAADVGQSDGR
                     NYGLIFSGEIRYSVTGKDSPVDTYVLIQAADTDLAFATSITSQTLAAGYTVADVNRAL
                     MKDFEAKGATEGLTPEMPATVYPRGRVLFGMTRHLMDNVAGQCGATWQFVDGQRQMVA
                     NNEYVHDAIVLNSATGLIGMPQQTIGNGVNVRALINPNIRVNGLIQLDQASVYRTALS
                     NNDIAMAGGQITDQNTDGNITLSGTTAQPASIATDGVYIVRGIMYTGDTRGQAWYMDM
                     MCEARGAADLYTQSALQR"
     gene            32921..33262
                     /locus_tag="RH057_001319"
     CDS             32921..33262
                     /locus_tag="RH057_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001214055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127718.1"
                     /translation="MRGIIFLLAVFSACSVWADGFTVKCGGYTMVANQGELSTINGER
                     VTSQKITELGTNGLKVDMGIMPAKDGNNYGFEYIRRPGTETRFLNVQLLQNSMDAPKI
                     IGSFPCKKVDG"
     gene            33431..34003
                     /locus_tag="RH057_001320"
     CDS             33431..34003
                     /locus_tag="RH057_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001766747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127719.1"
                     /translation="MAISYAFALATITQQMNQVQEAVNGAFKPLISNACELPQRLDAE
                     EVFRRCTAIAARAQEIENTAKEGMSHLEAFRNGRIIVDELPEEFLSHLEGLAKACRNA
                     KGHLVDMFSEAERSPMWQGHLQMLRPLKRKYVRALTAVENTATQLAAEVRQSQPFQAE
                     LLSDNVTREEAIELISTSHKMLGANAHKWM"
     gene            34005..34421
                     /locus_tag="RH057_001321"
     CDS             34005..34421
                     /locus_tag="RH057_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafO family
                     toxin"
                     /protein_id="HDV3127720.1"
                     /translation="MAKVSITGELRHLAAAHKYAQMLADYISKGSQFWCFGSLGGFER
                     NYDAMAANIRKIHLKLPGDKPWPPEASLSERTCDNFLVYAQHLYIDEHYQILAIISPN
                     AHQQADSMLPRLIKLAEETFIELPPDELEKLKTYDS"
     gene            34464..35216
                     /locus_tag="RH057_001322"
     CDS             34464..35216
                     /locus_tag="RH057_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HDV3127721.1"
                     /translation="MAVSDQTRSGDLAETFKSERETTKNQIRVALPGIIQSFDPDAVT
                     AVVQPAIRSVEKDNDGNRITKNYPLLVDVPVVFPRGGGCTLTFPIKAGDECLVVFADR
                     CIDFWWQSGGIQEPVDDRMHDLSDAFCIVGPQSQARKISGINTSATQLRSDDGSTYFE
                     LNPDTRKIKIVAPGGLDVVAPLADFSEKVTIHGLLTWMGGMVGSVVSGVASKITGVVE
                     FLGSVKANGKPIDDTHTHGGVQRGGSNTDGVN"
     gene            35216..35569
                     /locus_tag="RH057_001323"
     CDS             35216..35569
                     /locus_tag="RH057_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127722.1"
                     /translation="MRYRREDADGDYTFGSGDDTWLINSPEAVAQAVKTRFELWYGQW
                     FLDTTEGTPWIQSVLGKQKPETYNLAIRKRILETRGVKSILSFNTTVDTTTRRVMFSA
                     EIDTLYGITTVTSEA"
     gene            35569..36768
                     /locus_tag="RH057_001324"
     CDS             35569..36768
                     /locus_tag="RH057_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001197067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HDV3127723.1"
                     /translation="MALNLDSLGLSAKVTAEGISAPDYQTILSTLISYFQQIYGSDAY
                     LEPDSKDGQMVALMALAIHDANNMAITVYNCFSPATGYGAALTSNVKINGISRKGATN
                     STVDLLLTGTAGTTIINGSVKDSNNVIWRLPASVVVGVDGTVMATATCSVSGAVAALA
                     GTITEINTPTRGWVSVTNPAAATVGSPAETDAELRIRQSQSVALPSITPFEALDGAVS
                     NVTGVTRHKLYENDTGSEDGNGLPPHSVAVIVDGGDVTDIAQAIRGNKGQGTATHGTT
                     SVTVPDKYGNPHVIKFSRSSDVPVYARIKLKVFTGYTSQIGQQIQQAISDYINSLTIG
                     DSVLLSRIYSPANLGVVSGGNARYYDIQELTIGKSPGALSSSNIDIRYNESASCTPEN
                     IVITVES"
     gene            36765..37445
                     /locus_tag="RH057_001325"
     CDS             36765..37445
                     /locus_tag="RH057_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016239891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HDV3127724.1"
                     /translation="MSKYTELITNYHATKPKFLAHVDLMTRPLIDVAAATRGLITAFD
                     IDSAVGVQLDILGLWIGRSRVVSQPISGVYFSWDTDGLGYDQGVWQGPYDPDSGYMYL
                     SDETYRVILKAKIAINNWDGRNDSLPAILDAATAGSGLRMQIVDNQDMTISVWLFPDT
                     DISDVSRELIAAIKQGYLTVKAAGVWAGGIETPSVETPSEGSKFFGFDMDNEFISGFD
                     VGAWGVLL"
     gene            37445..38161
                     /locus_tag="RH057_001326"
     CDS             37445..38161
                     /locus_tag="RH057_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127725.1"
                     /translation="MAKNDFKAFATDRNANVMSQEEWEALPALLSGFTAGKASSAQVN
                     KVIRQASFIAAALAQFVSDKTQRDVLDNGDLPGFVELLGSGFAVEYLSRKNPFGDIKS
                     DGTVKTALENLGLGDISATGVATGSMGETGYAIFPMMIGGTRKTLIMQWGVMTSLRSG
                     GTMDINLPIAFPTMVMQAYATHDNVIQTARPTVYAIAPTSRTTITATAMSLRADSVGA
                     AVTRSSDSDLCYGRYLAIGY"
     gene            38163..38597
                     /locus_tag="RH057_001327"
     CDS             38163..38597
                     /locus_tag="RH057_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HDV3127726.1"
                     /translation="MNLKYFSPSENAFYAGELKESYIAADSWPSDALEVDESLYLEFI
                     DEPPAGKYRGVDDAGMPCWLEIPPPTAEEMASNAENMRQQLIDEANVYINKRQWPGKA
                     AIGRLKADELERYNLWLDYLDALEVVDTSSAPNINWPDPPDA"
     gene            complement(38569..38745)
                     /locus_tag="RH057_001328"
                     /pseudo
     CDS             complement(38569..38745)
                     /locus_tag="RH057_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
     gene            38745..38972
                     /locus_tag="RH057_001329"
                     /pseudo
     CDS             38745..38972
                     /locus_tag="RH057_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611553.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(39164..40321)
                     /gene="intS"
                     /locus_tag="RH057_001330"
     CDS             complement(39164..40321)
                     /gene="intS"
                     /locus_tag="RH057_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HDV3127727.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISVGIDPVKAKKASSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGAMERANKA
                     RRRCGEVFRYAIVTGRAKYNPAPDLADAMKGYRKKNFPFLPADQMPAFNKALATFSGS
                     IVSLIATKVLRYTALRTKELRSMLWKNVDFENRIITIDASVMKGRKIHVVPMSDQVVE
                     LLTTLSSITKPVSEFVFAGRNDKKKPICENAVLLVIKQIGYEGLESGHGFRHEFSTIM
                     NEHEWPADAIEVQLAHANGGSVRGIYNHAQYLDKRREMMQWWADWLDGKVE"
     gene            complement(40483..40557)
                     /locus_tag="RH057_001331"
     tRNA            complement(40483..40557)
                     /locus_tag="RH057_001331"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40522..40524),aa:Arg,seq:cct)
     gene            complement(40633..41565)
                     /locus_tag="RH057_001332"
     CDS             complement(40633..41565)
                     /locus_tag="RH057_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV3127728.1"
                     /translation="MDNDKIDQHSDEIEVESEEKERGKKIEIDEDRLPSRAMAIHEHI
                     RQDGEKELERDAMALLWSAIAAGLSMGASLLAKGIFHVELEGVPGSFLLENLGYTFGF
                     IIVIMARQQLFTENTVTAVLPVMQKPTMSNVGLLMRLWGVVLLGNILGTGIAAWAFEY
                     MPIFNEETRDAFVKIGMDVMKNTPSEMFANAIISGWLIATMVWMFPAAGAAKIVVIIL
                     MTWLIALGDTTHIVVGSVEILYLVFNGTLHWSDFIWPFALPTLAGNICGGTFIFALMS
                     HAQIRNDMSNKRKAEARQKAERAENIKKNDKNPA"
     gene            41859..42614
                     /gene="mlaA"
                     /locus_tag="RH057_001333"
     CDS             41859..42614
                     /gene="mlaA"
                     /locus_tag="RH057_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HDV3127729.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADGLYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
BASE COUNT        10955 a        10190 c        11085 g        10533 t
ORIGIN      
        1 gggcgaattc aaacggtgta atgtgttctt ttctgttggc attaaggtag tcagcccacc
       61 attgtaacat gagttttctc tcttcgagat gttccgcttt gtggatatat gctgcccgaa
      121 ccgagttacg ctcttggtgg ctcatttgac gttctacggc gtctcttgac cataacccag
      181 attcgattaa tgcactacaa gccatagttc gaaaaccgtg accacaaact tcagctttcg
      241 tgtcgtaacc catggtgcga agagccttgt taacagtgtt ttcgctgatt ggtttgtcat
      301 cgcgactaaa accaatgaac ataagatcct tttctccact tatagcttgg agccttttaa
      361 ataatactac agcctgctta cttagcggca caagatgagg ggttttcatt tttgagcccc
      421 tatttgaata cttaaccccc tccaattctc gtctttctgc agggattgtc cataaggagt
      481 ttttgaaatt cacctcagac caacgagcga atctaagttc acttgaacgg atgaaagtca
      541 gtagagttaa ttgaactgct atttgagtga tacctttccc tttataggca tcaatccgtt
      601 tgagaagctc aggaagtcgt tcgaggggaa gggcagggcg gtgattactc ttggcagtgg
      661 ttacagcgcc tgcgaggtcg taagctgggt tttggtcaat caaagcattg tgaacagcat
      721 accgcatgat ggcagtgatt ctttgttgta aacgggcagc aagttcaaga tgtccagttt
      781 cctctgcttt tttcagagga acaagtaaat cacgagtttt aagctcacta atgttccgct
      841 taccgataac aggaaataca tgagtgatca ggctgtttaa tacggtagtt ctgtgactgt
      901 ctgaccaggt tctgttgctt gagtgccagt ctctggcaac agtttcaaaa atgagtaagc
      961 ctttctcttc aatcttttct gtttttcgct tttcgccagg gtcaacattg ttggcaatga
     1021 gttgccttgc ttcttctctt ctacgacgag cttctgctaa agacacttcg ggataaacac
     1081 caagcgccaa cattttttgc ttaccagaaa agcgatattg aagccgccag tattttgaac
     1141 ccgtagggtg aaccaggaga tgcataccat ccccatctgt taacttgacg ggcttttcag
     1201 taggtttcgc atttctgact tttacctcag ttagtgccat tgtgacctcc tgatgatggt
     1261 atctgcatta tcgaacttac aataccaaca gttataccaa caaaacaagc tggatgtccg
     1321 tatatcccaa cagtccttgg tagaccatgg ttaaagtgta cttcattgaa atgattggga
     1381 aaaatagact ttgatatact tcggtagaca gggttctggc gtcccctgca gacatctact
     1441 tgaagcggca ggggattgat tggaatgata ttttttagat gtgagaaata ttttacccgc
     1501 tattttaccc attagcgcgg cttaagagct tatttttgaa ttcacaatgg tcacgatata
     1561 accatcttgc gcgtccgtgg ataactttgg cttttggcag gtcgccggac ttaatccggt
     1621 catagatgaa ggttttacca aagccagtat cggccatgat gaatttcaaa tcaaccagtg
     1681 aatcaggttg tagttcgtgt tgcatgagtg ctatctccga atagggaatc gaacctgcaa
     1741 atcaggcaag aaaaaaccgc catcaggcgg cttggtgttc tttcagttct tcaattcgaa
     1801 tattggttac gtctgcatgt gctatctgcg cccatatcat ccagtggtca tagcagtcgt
     1861 tgatgttctc tgcttcgata actctgttaa atggctctcc attcctccat tcacctgtga
     1921 ctcggaagtg catttatcat ctccataaaa caaaatccgc cgtagcgagt tcagataaaa
     1981 gaaatccccg cgagtgcgag gattgttatt cagtgctgat atttaccttt atagcgaaca
     2041 cctttaccgg tttatcgccg aagtgcggat gtgtgattgt tttcacttca tatcctccat
     2101 acggaacatc aattctgcga ctggagtcgt cgcgcttcgg atatcccttt gtgataatca
     2161 ggcggtcata ctcgcgtaac ataattcgct tattccagta gtcattacac aagcgatact
     2221 cttccgtttt ctctccgcga atcatggcat cgaagtattc acctttgaca gcaagttgca
     2281 ggttagccat taccgcacct ccagtctcca taccgcctga ccaatccggc tggcataggt
     2341 atctttggat actgctccgt ctttagccag ctccataaga attttgcgca aatctgccga
     2401 acgccattct tcatcaggaa attccttctc cattgccaac cgcagattcc aggttgctat
     2461 cgtgaatgga tattccccgc cgagagcttt ctcttgcagg gcagcccggg aacgcatcac
     2521 ctgcaaaacc ttctctttta catctatcat ttcgcctcct gcggcggctc tggtagtggc
     2581 atccagtgtg acggctcaca taccccctca acaccattca tgtaaaagaa ttgaaataac
     2641 cctttacctt tgtgaaaccc taccatctgc tcttttgtgt ctgaacaata aaccaaaaag
     2701 ctaccttttt tgtaggccac atacgcagtg caatctgact caatcaggct ttctgtaggg
     2761 atgtactggc aatcaacgtg ccacactgcc attgcatcca cgctatcagc gcaaacaggc
     2821 tgatcgatat ctcgcccaca attccaggct ttttgggctt cttccagcgt gtaaacatga
     2881 gcgcgatcga tatcagaact gtaaccattg ccgttatggc aatggaatga agcgttattg
     2941 cccacagttt cacgcgagca catcatgtaa aaacggttag tcactggctg cctcctttgc
     3001 gccacatcgc attcagatat ttgttttcat tcactgatgg aaaagaattt ctcttaagta
     3061 attcctctct cgatggcatt ggctttacgc gttggcgaat aatcatttct gccggaagaa
     3121 tgtcgggatt gtatgcaagt cctctcatgg taaattcctc agtcattact gatagcgcca
     3181 tagcgtgagc ggtaattacg caggcgcggg tcaatttcag ggaagtgggt atatgtggct
     3241 ttgcggaatg gtcggattga tgtctggtaa attcgctcgc gttcttcttt ctctgcaagc
     3301 catatacagt ggcgaaattc cttttcctct ttcgtttcct gcggtagaga cattattcga
     3361 tcgtagtttt ttctgaattt atccagcacc tccgatacgg aattgccgga acagcggcgc
     3421 gggtcatccg caccatacag aggcgctggc ataattaaat ccttattttt ataaatcaga
     3481 atgggatgga atcgtcgtat acaggagtgt tctgctggtt actactttgc tgctgcgggc
     3541 tgtttcctga agttgcaaat ccaatctttg cattcagtaa ttcaagagtg attgattgac
     3601 cattttgccc ctgataaaca tcaaccctga tgttttctcc ggtaatttcc acaatgccac
     3661 cctcaaccag aacgctacgg tagtaatccg cttgcgctcc cggcttggca aatataacgg
     3721 cgctgtagtt tgtccattct ttctttttag tctggcggtc gtaatactga acgccagcac
     3781 ggatgttaaa tccgatattt tccccggcct gaaactctct tgcgggcttg tttagtctta
     3841 cagtaatcga atgtgccatt aagcagccgc tccttctaat tcatctcgtc tgatgttgta
     3901 aacgtcctgc gctttgtgct gctccggtgt gccttcgagc atcttccacg ctttggcgaa
     3961 cgcctgttta agctcttcta cggtgttttt ctgcattgct gcgtcagtga atgcctttaa
     4021 aacctgttca ggtgtaggtg atggctttga ttgctttgct gctgcgttct gctgatgttt
     4081 atgctcgtct gtatctgcat ctttcgcatc atctatgccg aacaaaccat tgaggcaata
     4141 cttgcgtgca taagagcttg tagctccagt aacttgtgca gaatccattc ctttcttgct
     4201 ttcttcctct cgtgcaaggg cggttgccgt atgactgttt tcgccatcgg taatagttgc
     4261 cgtggctttc acgtaatacc gatcaccaat caacacaact tcatcgctga ttgataaaaa
     4321 caggccattc agtagcggct taacaccctc aagaatgtct tcgcagcttc tgtatttata
     4381 tttgccgaat gagttgtatt ggttctttgg cgcgttcaga ttctcctgaa tagctgccag
     4441 tcttgcgtaa aattctttgc tcatatgatt gttctcagaa tggacacggc ccaaggaaat
     4501 aacgctgatt taatacttca gtctttgccg catttaaaaa tacgcgaaca ccttcacgat
     4561 ctcccttctg gcgatacatt aacgcctgct gcgtgtacat gcgtctctgt aacttgctct
     4621 ccttcactgt ggttgcaagt gacatgaata tctccttcgt taccgattaa atctttcatc
     4681 tgacgaatga attcttcgtc tgaccagtta tctgtaaaac tcatttcctg cgatacaacg
     4741 gaaggttgat agctgatttc atcgctttat ttgcttcaag ccacattttt gaatcaccaa
     4801 taaatctggc tattactgct ttgttctgtg cagcacgaag catctggtga ttgatggcta
     4861 tttcattgcg cataacgcct ccagttgttt ctttgctgct ctgattaatt gtttaactcg
     4921 gcgtgataat tcagattcgt gcgggtagaa agcggacatg acgccgctac ccgcgagctg
     4981 aaagtgcatc atgggtaact ccttatattt gattgcataa cgaaaacgcc tcgagtgaag
     5041 cgttattggt atgcatataa aaaagccctc acactggagg gcaaagaaga tttccaataa
     5101 tcagaacaag tcggctcctg tttagttacg agcgacattg ctccgtgtat tcactcgttg
     5161 gaatgaatac acagtgcagt gtttattctg ttgtttatgc caaaaataaa ggccgactat
     5221 gcggcctcag aatggtttat atccattttt aaaggcgaaa tcgtatgcct catccttagt
     5281 gctgaagtat tcataatcag caaatccatc acgataaaac ttgcagcacc aatgatgcgg
     5341 aatgccatcc tcaggaccat agtcttgatg gaacacatcc tcgacgatga tattttcatc
     5401 ttttcccata tatcacctaa aataagtggt ttgctgccaa aacaatgaac catccggaaa
     5461 ttccagatag ttcataattc actcttcaat acttccaact tactaatcgc cgatagatat
     5521 ccgcgctgat agggaatcat cattccttcg agcttgccac ttcttaactc ctccctgagc
     5581 aattgtattg cttgatcaat aacctctgac ttagcgtcct ttatggcttg cttgcggggc
     5641 tttgctttct gctttggcag atttctcaag catgatggaa tgtatgtctg attcaccact
     5701 tacctcgccg tcagttgttt tgatttccgg tatcctgccg cgtaaatagc tacgtttggc
     5761 aggcaaacac ttccactgca ttcatctgcc ttcttgcagc gaaggcttcc gagtgatgct
     5821 gctttgtctg ctctgacgca accagagagc tttcgcgcaa tttttcgcgc cagtcgctgc
     5881 tcttgcattg cctgttcacg ttgagcctgt ctgcgtgctc tgcggcgatt tctggcgtta
     5941 tcgtcagcca gatatgtaat gactactgtc atgttgacct ccgatgattg actttggtga
     6001 ttggatggcc ggtgctgaat tccggcttac tggttagagc gcccgcacta ccagtgacgc
     6061 tgtcttgagg cgcagattgg ttactgcttg ccatgagcgc tgtttataca ttggtcgagc
     6121 atcagcctgc tcattcatcc aatcccaaag ccaactactc tttgactcac actctcgcag
     6181 tgagcgcgct catgcccttg agtctctgcc gcttcatcgc cgctgataac cggtgcgcgt
     6241 ctggcattcg cgctgcttta ccggagctac ttttaatata ttgaccctaa cccgatgcta
     6301 agcaggctcg ctcaatggcg actcagggca gcatcattac tgctgcattt cctttcggct
     6361 gcggtctaac cgcgttagtg caccattacg gcacctcctg tttggttaaa ctcagttccc
     6421 gcatttcggc gggacaatcc catcaatgtt aaagagcctg ccaatctgtt ccgtttggct
     6481 tccagcgtcc tgctgatggc taaagaatac tgtaggtatt ttattgtgta aatacccaag
     6541 gtatttattt ttgatgaaat aatgataaac aaatgaatac aaaggatatt tatttgtttc
     6601 ggtgtctgct tgttcagtgc tttttatgcg ggatatgtga agtggatccc gatagctatt
     6661 gctgccggga ttatgggtta gtcagcgaag gttaagacga gaattacctt aatgatgtct
     6721 gctacaacag acacggccat agataaacca aagacgatcc aagccataga gatgtcttca
     6781 ctaccatcgt atagggttcc gtaatcactg gtgtaaggcg taaatgtcgc gccttgatac
     6841 aataggtata agcttgatcc atagaggata aatgcagata tcccttgtat tgctatgatc
     6901 actagaatca tgaaacgagc tgatctatgc gcccaagcct ggcttatttt ttctgataga
     6961 gatttcgcaa taaaagcatg cgctaagccg taaattgttg agattgccaa catccccaaa
     7021 aagcttgcta tagcggttcc aaccataagc gcccccttgc gtgatcaaac cagtctgagt
     7081 tttgtctcaa ttgcaacgcc tataatcttg cagtttccat tgattggcac gagaggccat
     7141 gcaggattaa gtcccttgag gtatttattt ccgccgtcga ttatcagctt cttgaatgtt
     7201 gcttcgttag agtcagaaag ttttgctatg accaagctgc cgttgatcgc ctcccttccg
     7261 gtatcgaaaa gaacgaatgt tccctctgga atgcttaacc caaccggtgc cgtcattgaa
     7321 tcaccttcca ctttaagcca gaacgcatta ccttgaatat gcgcgtcaga ctcaagccaa
     7381 acatctatgt ctttaatggt gtatggttcg catgcttcac accacgagcc agcctggata
     7441 ctgcttaaca ccggatacct ctttcctgct ctgtattccc ctgcatacct tacgttggca
     7501 tcgctcttaa ggctttctgc ctgttctgca accttggcag caattgactg gctaaaatca
     7561 gcaattgaga cttgcaacag tcgtgcaaaa ccagatgcaa cctcaacgtt tagcgcgttt
     7621 ctgccattaa gataatgccc taccgctcct tgggtgatac caagttcatc agcgattgag
     7681 tattgggtta ttcccaactc tttctttttt gactcataca aagccttaag ccgcttagcg
     7741 tcttcgagct gttctgtcgt cagtgatttt ttattttcca tagcttaatt ctaatagcta
     7801 aggtacttaa actaaaaata ccctgagtat tgattgcttt gaatacctgt agtattcttt
     7861 gttcatggtt aataacggag tgtgcatatg attcgaatga cacttgccga ttacgccaaa
     7921 atccatggac aggctaaagc agccagtgac tttggtgtaa tccagtgcgc tatcagcaag
     7981 gccattctgg caggccgtaa cattatggtt acggtaaagc ctgatggcag tgtgattgga
     8041 gaggaagttc gtcctttccc aagcaacaag aaaaacaaat agtaacaccg ctctttaaca
     8101 gtcatggtcc tcattcccgc cgaaatgcgg gaatacaacg cgcatcagtt gttgcgcata
     8161 acttcttatt tgttaaggaa atacttacat atgcaactta caagtactcg caagaaagcg
     8221 aatgcaatta caagcaacat cctaaatcga attgctgtac gtggtcagcg aaaggttgct
     8281 gatgcattag ggatcaatga atcgcaaatt tcgcgatgga aagacagctt tatcccaaag
     8341 atggccatgc ttctggctgt gctggagtgg ggtgttgaag acgaggaatt agcggagctg
     8401 gcaaagaaag tagccatggt gctgacaaaa gaaaagcctc aagactgcgg caacagtttt
     8461 gaggcctgat gtagaaagac tggatcaatc cacaggagta attatgacaa aacgtcgtaa
     8521 gaaataccag gaaaaagaag agattcgaca ccctgattca cctgagggat tagtggtagc
     8581 cgcagcaaat aacagggcgt tcgcagagcg ccttgttggt gtttacagac tagccaaagc
     8641 aggagtgaaa catgggcgtc gttaagttag ctgattacag gcataaccct gtacaacatc
     8701 aggaggcatc cagtatgggg tatgtctcta tacaccgcca gtttatggac agcaggctct
     8761 ataaggactc tcaggcagta catctttggc ttcacttaat cctcaaggct aatcacgaat
     8821 ctactgtcgt caatacggat atcggtccga taactgttga tcgcggtcag atgataactg
     8881 gacgcccgtc gctggtcaga gaaacattca tccccgacaa caaagttcgg agcttattgc
     8941 ggacttttga gtcgaaagga atgcttaata tttgctcgat ggggaagaaa tttagcctgt
     9001 ttacaatcgt taaatatgac gattttcagg caaaaaattg tccaacggtt gtccaacggt
     9061 tgtccaacgc aaacagcagt aatggcgcgg ctctcagcgg agattatcca acggttgtcc
     9121 aacggttgtc cataaacaat aatataaata atatctctaa tactgacgta ttagagagtg
     9181 ccacagcaga caaaaagtct gacaagaaaa aaccttccgt tagctgtcag gatgttgtcg
     9241 atgcttacca cgaaatcctt cctgaagcgc caagaatccg cgcactgaat gacaagcgta
     9301 aaaaccagat ccgaacgttc tggcgcaaag ccggagtgat aacccgccag cttgacgggc
     9361 atgggttcac gatgcaggac tggagaaatt atttgagcta cgtaggcgaa aattgccgat
     9421 ggatgttcga agagcgccca aaccatcagc gcggaaccgt ctggcacaaa aagggatttg
     9481 atttcctgct taacgataat acctacctga aagttcgtga gggtgaacac gatgaccgat
     9541 aatttttatg cgccgcccca tagcatcgag gcagagcagg cggtgattgg tggattgctt
     9601 ctggatgatg acagcagtga gcgcgtccag aaagttctgg cgatgctgaa gcctgattca
     9661 ttttacagcc gaccacacaa aatcattttc gaagaaataa ccagaatgca ccgggagcaa
     9721 aagccagtag atggcctgac gcttttcgat gaactggagc gtaaatcgtt aacggcgtct
     9781 gttggcggtt ttgcttatat cgctgagatc gcaaagaaca cgccaagcgc cgcaaacatc
     9841 gttgcctatg caatgcaggt tcgcgaaacc gcaatggaac gctacgccat caaccgcatg
     9901 actgaagcga cggaattgct atattcccgc aacggaatga ctgcaacgca gaagtacgaa
     9961 gctattcagg cgattttcac gcaactgaca gaccatgcaa aaaccggatc gcgtcgcggc
    10021 cttcgctcat ttggtgaggt catggaagac tgggttagcg accttgagaa gcgatttgac
    10081 ccgtcaggcg aacaacgagg aatgagcaca gggatcccat cgctggacag gatgctgtca
    10141 ccgaaaggtc tggtgaaagg ctctctgttt gtcattggcg ctcgccctaa gatggggaaa
    10201 acgacgctat acagccagat ggcaatcaac tgcgcagtgc atgagaaaaa gcccgctctg
    10261 atgttcagcc ttgaaatgcc aggtgaccag atactggaaa aactggtggg acagaagtca
    10321 ggtgttaacc cgaatatttt ttaccttccg gcgacaaatg acgctgatga cggctatcag
    10381 ggtgattacg atggtgactt caacagggcg atcgaaacag ctaatcgttt gagtgaaatc
    10441 gacctgctat acatcgacga cacgccggga ttatctctgg ctcaaatcgt tagcgaaagc
    10501 cgtcgaatca agcgagaaaa aggatgtgtt ggcatgattc tggtcgatta cctgacacta
    10561 atgactgctg agaaggccga tcgcaacgac cttgcttacg gcatgatcac caaaggactg
    10621 aagaaccttg ccaaagagct tgattgcgtt gttgtgcttc tgacacagct taaccgcgca
    10681 ctggaaagcc gaaccaataa acgcccatta ccaagtgact cgcgagatac agggcagatt
    10741 gaacaggatt gcgattattg ggtggggatc catcgtgaag gcgcttttga tgacagtgtt
    10801 ccacctggtg aaactgaact aatccttcgt ctcaatcgtc atggcaatac cggcacggtg
    10861 tattgcattc aggcaaatgg cgctatttat gacacagacc aacagtctgc tgaaatgcgc
    10921 cgccgtgaac gcgaggaacc gcagtccaag aagaaaggag gattctgatg accatctaca
    10981 tcactgagct aataacaggg gctatttaca cagtagccct tttttattgg attaagaacg
    11041 agggggatcc tgatggacac cgttaacgga atgtgttcag acgcaccgcg tgccaaaaaa
    11101 tgtaaatgcg gaaaatcacc gacaatattc gacatggaga acgggtgcca aatctactgc
    11161 gctaaccacg ctgctgtggc ggccgcgaat tatcgcagtg cggtaacgga gtggaataac
    11221 ctgaaatctg ttagagaggg aagtcatgaa aaaactaacc tttgaaattc gatctccagc
    11281 acatcagcaa aacgctattc acgcagtaca gcaaatcctt ccagacccaa ccaaaccaat
    11341 caatcaaata actcagcaga gacttaaaga agtccttctc tacgatgagt taacagggaa
    11401 attcacatgg aaagtaaaga aatgccagag aatgaatgct ggggatatcg cagggcataa
    11461 gagtagcgaa ggctactggg taataaaagt cgatggaaaa ctttataagg cacacaggct
    11521 ggcgtggctc tatatgaatg gcagtctacc aaaaagtgat atcgaccaca taaatctcgt
    11581 tagggatgac aatcgtatag caaatcttag attggcaact cgatctcaaa acatccaaaa
    11641 cgtaaacaaa aaggcaaata acaaatcagg atataaaggc gtttcatggg ataagaaatc
    11701 aagaaaatgg agggcgcaaa tcgtaatcaa caagaggaaa gttaatcttg ggttttacga
    11761 tgacccaaag gaagctcata aagtatatgc aaataaagcg gatgaatgtt tcggcgaatt
    11821 tgcgaggtat taaaatgaaa tgtgtcaaat atcaccttac taatgaatct ataagatata
    11881 acgccattca gtatcttagg acatgcgatt tagacatcat tgtcgagttt aagcagcgca
    11941 accgcagctt agaccaaaac aggaagcttt gggcttgcct tggtgacgtc tctcgtcagg
    12001 ttgaatggca tgggcgctgg ctggatgcag aaagctggaa gtgtgtgttt accgcagcat
    12061 taaagcagca ggacgttgtt cctaaccttg ccgggaatgg ctttgtggta ataggccagt
    12121 caaccagcag gatgcgtgta ggcgaatttg cggagctatt agagcttata caggcattcg
    12181 gtacagagcg tggcgttaag tggtcagacg aagcgcgact ggctctcgaa tggaaagcgc
    12241 gatggggaga tcgggctgca tgactatcaa atcaaatacg ccggcacacg acaaggactg
    12301 ctggcaaacg ccgctttggc tttttgatgc actggatatt gagtttggat tctggctgga
    12361 ttcggctgcg agcgacaaaa atgctctgtg cgctcactgg ctaactgagg ccgacgacgc
    12421 gctcaattct gagtgggtaa gtcacggtgc aatctggaat aacccaccgt acagcaatat
    12481 caggccgtgg gtggaaaaag ccgctgagca gtgcatacaa cagcgacaga cggtagttat
    12541 gcttgtgcca gaggatatgt cagtcggatg gttcagcaag gctctggaga gcgttgacga
    12601 agttcgcatt atcactgatg gacggattaa ttttatcgaa ccatcgacag ggctggagaa
    12661 gaagggaaac agcaaaggct ccatgctgct gatttggcga ccgttcatca gtcctcgacg
    12721 gatgtttact accgtatcca aagcggcatt gatggcgatc gggcagggcg tcaggagggc
    12781 agcatgaggc gacagcgacg aagtattacc gacatcatct gtgaaaactg caaatacctt
    12841 ccaacgaagc gctccagaaa taaacgcaag ccaatcccaa aagaatctga cgtaaaaacc
    12901 ttcaactaca cggctcacct gtgggatatc cggtggctta gagaacgtgc gaggaaaaca
    12961 aggtgattga cccaaatcga agttacgaac aagaaagcgt cgagcgggct ttaacgtgcg
    13021 ctaactgcag tcagaagctg catgtgctgg aagttcatgt gtgtgagcac tgctgcgcag
    13081 aactgatgag cgatccgaat agctcaatgt acgaggaaga agacgatggc taaaccagcg
    13141 cgaagacgat gtaaaaacga tgaatgtcgg gaatggtttc accctgcatt cgctaatcag
    13201 tggtggtgct ctccagagtg tggaaccaag atagcactcg aacgacgaag taaagaacgc
    13261 gaaaaagcgg aaaaagcagc agagaagaaa cgacgacgag aggagcagaa acagaaagat
    13321 aaacttaaga ttcgaaaact cgccttaaag ccacgcagtt actggattaa acaagcccaa
    13381 caagccgtaa acgtcttcat cagagaaaga gaccgcgact taccatgtat ctcgtgcgga
    13441 acgctcacgt ctgctcagtg ggatgccgga cattaccgga caactgctgc ggcacctcaa
    13501 ctccgatttg atgaacgcaa tattcacaag caatgcgtgg tgtgtaacca gcataaaagc
    13561 ggaaatctcg ttccgtatcg cgtcgaactg attaaccgaa tcgggcagga agcagtagac
    13621 gaaatcgaat caaaccataa ccgccatcgc tggactgtcg aagagtgcaa ggcgatcaag
    13681 gcagagtacc aacagaaact caaagacctg cgaaatagca gaagtgaggc cgcatgacgt
    13741 tctcagtaaa aaccattcca gacatgctcg ttgaagcata cggaaaccag acagaagtgg
    13801 cacgcagact gaaatgtagt cgcggtacgg tcagaaaata cgttgatgat aaagacggga
    13861 aaatgcacgc catcgtcaac gacgttctca tggttcatcg cggatggagt gaaagagatg
    13921 cgctattacg aaagaattga tggcagcaaa taccgaaata tttgggtagt tggcgacctg
    13981 cacggatgct acacgaacct gatgaacaaa ctggatgcga ttggattcga cacccaaaaa
    14041 gacctgctta tctcggttgg cgatttggtc gatcgcggtg cagagaacgt cgaatgcctg
    14101 gaattaatca cattcccctg gttcagagct gtacgtggaa accatgagca aatgatgatt
    14161 gatggcttat cagagcgtgg aaacgtcaat cactggctac ttaatggcgg tggctggttc
    14221 tttaatctcg attacgacaa agaaattctg gctaaagctc ttgcccataa agcagatgaa
    14281 cttccgttaa tcatcgaact ggtgagcaaa gataaaaaat atgtcatctg ccacgccgat
    14341 tatccttgtg acgaatacga gtttggaaag cctgttgatc atcagcagtt aatttggaac
    14401 cgcgaacgaa tcagcaactc acaagacggg atcgtgaaag aaataaaagg cgcggacacg
    14461 ttcatctttg gtcatacgcc agcagtgaaa ccactcaaat ttgccaacca gatgtatatc
    14521 gataccggcg cagtgttctg cggaaacctc acattgattc aggtacaggg agaaggcgcg
    14581 tgggcataag agaactaaac ctcaccaaag aacagcatga gtggctgaat ggctggcttg
    14641 aactgtgggg cgcatgggtt tattcaggtc gtctggaaaa gcgcatgagc agcgtaatag
    14701 cgaagttcat ggagagcgta gagccgggaa gagttatgac aaggccaatg tgtaatgatg
    14761 atgatggaat gttgatttct caggtcgtcg attccgtcat gtacattgac aagaaagcct
    14821 ttggcatcct cctcagctac tacgctcatg gttcatctaa gcgagcaatt gcatcctact
    14881 atcacgcgac tgcaaagcca cgcaagatgt gtggacgtgg tggcgaggga tggagaaaac
    14941 cttcaatggc aacctgtaga aacgaaattg acgacatcct gaaagcgtcg ttatttgttt
    15001 tgtaccagcc aatgcaaaat gctttcaaaa tgcgtaaacg tgttgagaaa gttaagcatg
    15061 ttgctgttaa aagtcttgac atgcaattag ccatttagcc ataattagca ggtaagctgc
    15121 cgttagtgac tcttaagttg caacggtggc tttttttatt tgggtcagtc gtataaaggt
    15181 cattacggaa ggctgttaac cttcttatcg tggttcgagt ccacgctgtc ccgccaaata
    15241 tgctggttta gctccaatgg tagagcagtc gccttgtaag cgaatgggta acggttcaag
    15301 tccgttaacc agcaccataa ctgagccgta gccactggct atcctgaatt catcagtgat
    15361 agttacgctg cggcattcta cgcatgacct tcgtgaaagc gggtggcatg aggttgccct
    15421 aacaacctcc tgccgttttg cccgtgcata tcggtcacga acaaatctga ttactaaaca
    15481 cagtagcctg gatttgttct atcagtaatc gaccttattc ctaattaaat agagcaaatc
    15541 cccttattgg gggtaagaca tgaagatgcc agaaaaacat gacctgttag ccgccattct
    15601 cgcggcaaag gaacaaggca tcggggcaat ccttgcgttt gcaatggcgt accttcgcgg
    15661 cagatataat ggcggtgcgt ttacaaaaac agtaatcgac gcaacgatgt gcgccattat
    15721 cgcctggttc attcgtgacc ttctcgactt cgccggacta agtagcaatc tcgcttatat
    15781 aacgagcgtg ttcatcggct acatcggtac tgactcgatt ggttcgctta tcaaacgctt
    15841 cgctgctaaa aaagccggag tagaagatgg tggaaatcaa taatcaacgt aaggcgttcc
    15901 tcgatatgct ggcgtggtca gagggaactg ataacggacg tcaaaaaacc agaaatcatg
    15961 gttatgacgt cattgttggc ggagagctat tcactgatta ctccgatcac cctcgcaaac
    16021 ttgtcacgct aaacccaaaa ctcaaatcaa cagcagccgg tcgctatcag cttctttccc
    16081 gttggtggga tgcttaccgt aaacagcttg gactgaaaga tttctctccg aaaagtcagg
    16141 acgctgtggc actgcaacag attaaagaac gtggcgcttt accgatgatt gatcgcggtg
    16201 atatccgtca ggctatcgat cgttgcagca atatctgggc gtcgttacct ggtgcaggtt
    16261 acggtcagta tgaacataaa atcggtgacc tgattgcccg atttaaaaaa gctggtgggg
    16321 tagtaaatga agctgagata taggctggtt attgttgcct tcgttgttac cgtcattggt
    16381 tccttcatct ggtctgctgg gcattactac agcaaatatc agcacgaaaa ggagcgtgct
    16441 gatgaggctg tacgaaatgc tgaatcagca actgccatta cccgtaacgt tctgcaatca
    16501 ctgcaaatca tcaatacagt tatagaggct aaccagcatg caaaagagca gatcgcactg
    16561 gacgcatcgg gagcctcggc tgatatccgg gttgctgttg cgaatgatga ttgcactaat
    16621 cgccctgtcc ctgctggcgc agttaagcgg ctgcacgaat acgcgaacgg tctacgtgcc
    16681 ggttccggta gttccattac cagccagcct gacggctgaa acaccccagc cagaattacc
    16741 cgacccgttt acgtggggag caagtcttga tctgaatgtc gcgttgctgt cagcgctggc
    16801 gcagtgcaac agggataagg ctgatatcag gactttcgag aacaacaggg caggacaaac
    16861 cgatggcacg attaaacgtt gaagttaccc caccagacag cgaggcgctg aacgggattt
    16921 ttgcagagat tgagcgcaaa tatgcgcgtc agccgctgac accaaaagta attgatgaaa
    16981 tgcaacgcga agcgacgcgc cttgtacggc gaatgataac cacaaaggtt acgttcgtcc
    17041 gggactgaca ttacagaagc ccttcacact gaggggcttc gataatggag cactggaatt
    17101 attcatgaac agaccacacc caccatcgca ttttacgatg ccacctgacc cgaagccgta
    17161 catcagcata atgcccgcca gtgacgttgg cgagtggctg aatcagcaca tcctgagcga
    17221 tgagggtgac ctctacaacc ctgatcacca gcatttgctt gaagcggatc tgtgctttct
    17281 ctgggcgtcg aacgctttcg agaagaaagg gcgttccgtg cttgggcagg cggaagaagt
    17341 ggcaatgcgt gccggaggct ggcaaaaagc gcggatggag cagcagatgt atgaatggtt
    17401 cggcagggtg ccgcagttca tcatcacgct ggcagccgat tactgttcgc aatgttccga
    17461 tctggaattc tgcgcgctga tagagcacga gctttatcac atctgccagg cgacagatga
    17521 atttggcgcg ccgaagttca cgcaggaagg gcagccaaag ctgaagctgc gcggccatga
    17581 cgtggaagag tttgtgggcg tggttcgccg ttacggtgcg agccgggacg tgcaggaaat
    17641 gattgatgtg gcgaatcagc cagcggaggt tgctcatctc gatattgcca gagcgtgcgg
    17701 gacgtgcatg ctgcgactgg cttaaatact ggactgtata agacgaatgg tgatttatgg
    17761 ctgcattaaa atctgatgtg aaagccttca tcattcagtc gcttgcgtgc tatgacacgc
    17821 catcgcaggt ggtcgaggct gtccaaaaag aattcgggat caagatcacc cgccagcagg
    17881 ctgaatctca cgaccccacg aaggccagcg gtaagacgct cgccaaaaag tggatcgaga
    17941 tgttccacgc gacgcgcgaa cggttcctga ccgaaaccag cgacattccg atcgcgaaca
    18001 aatcatatcg cctccgcgtg cttgaccgca tggcaaccaa aaccgagggg atgaaaaact
    18061 tctccctgac ggcgcagctg attgaacagg ccgcgaaaga ggttggcgac gcttacacca
    18121 ataagctgaa ggttgaaagc actggcaagg atggcggccc aatcaagacc gagacgacca
    18181 acctcaccgc agatcaggcc gcagagattt accgcaagat gatggggtga tcatgcctct
    18241 cccgtttgaa ttcgatttca gaaaccctga ttaccagatg gtttttgaat ggcggatgga
    18301 gcgcttacag cgcattcgcc agaaccctga aatgctgcca gcgctaaagc agttttaccg
    18361 caccaacccg gcacagttca tcatcgactg gggtatgacg actgacccgc gtaacatcga
    18421 ttatggcctg ccggtcacca tcccttttct gctgttcccg aaacaggaag agtggattca
    18481 ctggatcatg gagcggcgcg aacgactgga gaacggcatc accgaaaaga gccgcgaaat
    18541 ggggctcagt tggaccgcga tcgggctggc ctgctcgctt tgtctcttca acaaagaaat
    18601 ggttatcggt ttcggctccc gtaaagagga atacgtcgac agcaccggtg acccgaaggc
    18661 gctgttctgg aaggcgcgaa agttcgtgga aacactgccc atcgagtttc gtggttcgtg
    18721 ggacgagaag aagcatgcgc cgtatatgcg cgttgagttt ccagatactg gcgcggttat
    18781 caaaggcgag gctggcgaca atatcggacg tggtgaccgt accacgcttt atctggttga
    18841 tgaggctgca ttccttcagc gtcctctgct gattgatgcg gcgttgtcac aaacgacgcg
    18901 ttgccgtatt gacctgagtt cagttaacgg catggcgaac ccgttcgctc agaagcgtca
    18961 tggcgggaag ataccggtat tcacattcca ctggcgggat gatcctcgca aggatgaaga
    19021 gtggtatcgc agggaatgcg agaaaatcga taatccggtg gtggtggcac aggaacttga
    19081 tctgaactac agcgcatcag cggaaggcgt cctgattcca tccgaatggg tacaggctgc
    19141 cgttgatgcg catatcaaac tgggtatcca gccaacaggc aaacgacttg gcgcgatgga
    19201 tgtcgccgac gaaggcaggg acaaaaatgc cttttccacc cgtcatggct tcctcctgga
    19261 gaatgtgcgg gaatggtccg gtgtgggcag cgacatttat cagtccgtcg agaaggtctt
    19321 cggtttttgc gaacaggaca acctcgaaga gtttcgcttt gatgaggacg ggctgggcgc
    19381 tggcgttcgc ggcgatgcac gcgctatcaa cgaacagcgt aacgctgcgc gtctaccgtc
    19441 aatactcgct acaccgtttc gcggtagcgg cgcggtgttt gatccggacg acgaagcggt
    19501 gcgcggtgac aacgggcaag ccgcacgtct gaacaaggac ttcttcgcta acgccaaagc
    19561 ccagagctgg tggcggttac gtaaactttt tcagaatacc tggcgcgccg tggttgaagg
    19621 tatggcttac aacccggacg aaatcatctc aatcagcagt agcatggcac tcaaagataa
    19681 actcatcatc gagctttcgc agccgaccta ttccattaat ggtgtgggaa aaatcgttat
    19741 tgataaacag cctgatggaa cccgatcgcc aaaccttgcc gactcggtga tgatcaacta
    19801 tgccccaatg aattcagccc tgaacatctg ggagctgcta gggagacagg cctgatggca
    19861 cgaaacaaac aagccctgcg gcgaactgcg caggccaccg ctgatggcta tgagaacttt
    19921 attgcccgcg tagggatgca gacgcctaac cagcactcag catcgaccta ccgggctaac
    19981 ttcaccagcc gcaaccgcat gctggtggaa tggtcctatc gttcgtcctg gatcatcggc
    20041 gaagcggtcg acgctatccc ggacgatatg acccggaaag gcattcgcat cacttcggag
    20101 attgacgcca aagaccgtgg caccctggaa gcgcagctgg atgagttgca gatctgggat
    20161 gcgctgaacg acgtgctgaa atggtcgcgc ctctacggcg gcgcggtcgg cttcatcatg
    20221 atcgaggggc aggcaccaat gaccccgctg cggctcgaaa ccattggaga aggcaagttt
    20281 aagggcattc tcccgctcga ccgctggatg attaacccgg tgctgacccg ccgcattaaa
    20341 gatatggggc cggacctggg taaacctgag ttttacgacg tggtgaccac tgcaacgggc
    20401 atcccggcct ggcgcatcca tcacagccgc ctgattcgct tcgatggcgt cacgctgcca
    20461 ttccagcaga agatgaccga aaacgaatgg ggaatgtcgg ttgtagagcg tatctgggat
    20521 cggcttactg cgttcgacag cgctactgtc ggcgcggcgc agctggtcta caaggcgcat
    20581 ttgcgtacct acagcgtgga gaagctacgc gagcttatcg cacttggtgg tcctgcgtat
    20641 gaagcgttgc tgaagaatat cgacctgatt cgacagttcc agagcaatga aggcatgacg
    20701 ctcatggact cgcgggataa gtttgaaacg catcagtaca gcttcagtgg tctggatgac
    20761 atcctttcgc agtttgcaga acagattagt ggcgctgttg gtattccgct ggtgcgcctt
    20821 ttcgggcagt ccccgaaggg attttctacc ggcgatgcag accttgccaa ctattacgac
    20881 cgggtgagct cgttgcagga acgccgctta cggatgccga tgcgtcggat actggacatt
    20941 atgcaccgct cggaactcgg taagccgctg ccggacgatt tcacgtttga gtttaacccg
    21001 ctatggcaaa tgtctgacgt tgaccgctca acggtggccg taaacaccac caacgcgatc
    21061 agcaccgcgc tgggcgacgg attgatgacg cgtaaggcgg caatgaccga cctgcgcgaa
    21121 aactctgacg tcaccggcat cggggcatcc attaccgacg aggacataga gaatgccgaa
    21181 gacgaagcgc cgccaggcat cggcgaactt ggcgacaaac cgccagagtc gccaggcgga
    21241 aatccgatat cgaacgagcc tacggcagat agcgcgggcg gtcggggata tcgtaaatgg
    21301 tcgctacgat ggttcaaatg acagtgtcac cgaaataatg gatgcgctgg agcgctacag
    21361 cgaaatcatc accccctggg cgacgaaggt tgctgagaac tttaccgccg acattgcacg
    21421 ccagaatgaa aagcagtggc gtcagcacag caggaacatc agcgcagagc tgcgcaacat
    21481 ggtcgaccgc gccccggtag gccaggtgat gaaatccatc gtcgccgagc aaattaagta
    21541 catcaagtca ctgcctcttg aggccgccga tcgggtgtat gacattcaga acaaggccat
    21601 cgaggccgtt gtgtctggtg gccgcgctga accattcgcg aaagagatag cagcgtccgg
    21661 tgacgtgtca cgctcacgag cgaaccttat cgcccgtacc gagcttggac gtgcaaccgg
    21721 cgcgctggat caggcgcgtg cgctgtcaat cggctcgaat ggttatatct ggcgtacagc
    21781 cgaagatggc gacgtccggc attctcatcg ggagatggaa ggtaagtttg tcgaatgggg
    21841 caaacctcca acgcttgatg gcatgaccgg tcacgctggc gagctcccga attgtcgctg
    21901 ttataaagaa atcgtttttc ccaaccctca ttcttatctc gcctgaatcg caggtaaacc
    21961 atgaaatatt ttttcaatac ccggctgggg gaaacccgct atcagctggc tgacggctcg
    22021 ctgctgtgca aagacgtgcc gataggtcga acgggtaagc agctctacgg cgctgccgat
    22081 ctgccaaacc tcaaacccga caagctcggt gagatagtcg taacgcgttc tcctgagcag
    22141 gtattccatc cggccacgct cgcctcattc gaagggatga gcatcacgat cctgcatcct
    22201 gaagatgaaa acgggaatgt gcggctggta aatcccgaga actggaaaga gcttgcggtc
    22261 gggcacctcc agaatgtccg gcgcgggacg ggtgagcagt ctgatttgat gctggctgac
    22321 cttatcgtca aagacgaaaa cgccattcag cttatcgaag atggcctgcg tgaagtgtcg
    22381 tgcggctatg acgcggagta cgagcagacc gagccaggta aagccgagca ggtcgatatt
    22441 accggaaacc atgtggctct tgtccccaaa ggcagagccg gaaatcgttg tgcaattgga
    22501 gacagagaca caatggcaaa tcaaaagaaa aactggtgga accgcatgcg tgcagccatc
    22561 aagacaggag atgccgacac catgaacgaa ctggtggagt cggctcccgc atcggttaca
    22621 ggagatgagg gggatttgcc gcagggcgtt aatctcaaca tcaacctgtc cccgcagcaa
    22681 ccactaccgg acaaagcacc agagatgggt ggaggtccaa ccggcgacag tgatgatgac
    22741 ctcaaaacat tactgaaagc cctgctggct aagctggaag gaaatgcgac gggcgataac
    22801 gacaataagc ctgacgataa tccgaccggt gacggcgagg acgatgaaga ggaaaccacg
    22861 attactggtg actcagcctg gcgtgccgaa gttatcgttc cgggtatcga tctgagccgt
    22921 aagatgaaac cgaccgcgtt caaacgcgag gttctggctt ccgcagataa aacgctggtt
    22981 cgccagatcg tcggtgatgc ggatatccgc aaattgccga aacaatcggt cgacatggcg
    23041 tttaatgccg tgtctgagat tgccaaaggg cgaaacaccc gcgccaccac cggcgatgca
    23101 cagcgccaaa atatgggcat gaccagcatc gcttccctga acaaacaaaa cgccgaattc
    23161 tggtctaacc gcaaaggata atccaatgac tgcatatctg taccggatgc ctgttggcat
    23221 tgccggggct atctctcgcc cgcaggactt aaccgtcgaa ccggtggtcc ttaaatccga
    23281 taacgccttc gctgcctatg ggctggctgg taaatacgat gatgacggtt ttttcgtgcc
    23341 gctggcagat ggtgataccg cagacaaggt gaaggggatc tatgtgcgcc cttatccgac
    23401 cacttcgcag ccggacatgg ttcgccaggt ggggagtggc aagaacttcc cgggcgacgc
    23461 aatgaagcgt ggctacgtga ccgttaatct cggttctgat tttgatgcca gcaccatcaa
    23521 aaaaggcgac ccggtatacg ttgtcgtctc cactgatgaa tccatcaaag tgccgctggg
    23581 tggattcatg gccacgtcag tcagtggcaa aaacgtggtg ctgaccaacg ctgaattcac
    23641 aggtgccggt gatgctaacg gcaatgcaga aatttcctgg aagatttaag gaacagacga
    23701 atgattactt ttgatcaggc aaccgttgac agctctggtg cctttctcat cggggagctg
    23761 gaacgactcg accagacgct gaacctgcca ctggtggggt acacctggac ccgcgatatc
    23821 caactgcgtg aagatgtctc catcgcagat gacatttcca gctggacgaa taccagcttc
    23881 gccgctgcgg gtactggcgc aaatccgaat ggcaaaaact gggtaggcaa agactcaacc
    23941 gctattgctg gcgtgaacgt ggatatcggc aaatccggta acccgctgaa cctgtggggg
    24001 atggaacttg gctggacggt catagaattg caggctgctc agcaggtcgg acgcccgatt
    24061 gatacgcaga agtatgacgg gatgcaactg aaatggcaga tggataacga tgaacaggtg
    24121 tatgttggcg attccgcatt aaacctgaaa ggccttgtta ccctgaacgg tgttcctgtc
    24181 aacaacgctg ccaaaacgtg ggcaacctca acaccggacg aaatccgcgc aagcattaac
    24241 caggtgctgt ctgatgcgtg ggccgcttct ggttactctg tggttccgcg tgatttgctg
    24301 atcccacctg agcagtttgc tctgttgtcc agcatcatcg tttcatctgc gggtaaccag
    24361 tccctgttga cgtaccttca gaccaacacc atcagctatc accagaacgg tgttccgctg
    24421 aatatccgcg cggttaaatg gctgaaaggc cgtggtgtgg ggaataagga tcgcatggtt
    24481 gcgtacacca acgataaaaa atacgtccgc tacccgctgg ttccgcttca gagcgtgccg
    24541 gtgcagtatc gcggtctgta tcagatcgtc acttactacg gcaagctggg tgcagtcgag
    24601 ccagtgtaca aagaaaccat ttcgtacgtt gatggcattt aacagccaca tggcccctgg
    24661 cggggccatt aaggatgacc tgatggcaaa aaataatgca gtaatacacg tacatacccc
    24721 gtttgtgctc acgcttcccg acggttcacg gcgcgagttt cttaaaggcc gtcatgctgt
    24781 ggaggaagac gttgccacgc actggttcac tcgtgcgcac gcggaggtat ccgttggcaa
    24841 agccacagac gcgcgtaacg aggtaaaaaa tgccaaagaa tcaaagtctg ccagcggtaa
    24901 gtgattttcg ccgcgacttc ccgcagtttg ctgaccctgc caaatatccc gaagcgcaaa
    24961 tccagtttcg tctgaatctg gccgatgaac tgctgagcga aaacgtcacc ggcaaaaagt
    25021 tgtttccgta ctttgccgga ttgttcgttg cacactacat gacactctgg gcggcagaca
    25081 gccgggcgat gctggctggc ggtccgggcg gttcaaccaa tggtgttcag tcctcaaagt
    25141 ccgtggataa ggtaagcgtc agctatgaca ccagcgcgac gctaaaccat gacgcaggct
    25201 tctggaataa cacccgatat ggcgctgaat tttatcagct gatcacgatg ttcggtgcgg
    25261 gcggtcgcca gctatgagct tcaaaagcgg tgtaacaacg agggtggata acgctcaggc
    25321 cattctggat gcgctcaaat ccatcagtaa aaaagaagtg ctggtgggta tcccggaagc
    25381 agacagcgag cgtgaggatg ttccgtttgg taatgccggg atcggttacc tcaacgaata
    25441 cggctcgcca gcgcaaaaca tccccccacg cccgcacctg atccccggcg ttaaatcggt
    25501 agaggaacag acgttgccgc agctcaaaac agcggcgcag gctgcgcttg atggaaatgc
    25561 ggcgggggcg gaaagagcgc tcaaccgcgc cggaacgctg gccgctaatg gcgtcaggcg
    25621 ttacatgact attaccggct ttacaccgct tgctgatagc accgttgaag cccgcgcgcg
    25681 tcgagggcgt aaaggggcga aagcggagct tgcccgacgc gctgctggcg agtcccccgg
    25741 aaccgatctg gtgaaaccgc taatcgacac cgggcaatat cgcagagcga ttacccatgt
    25801 tgtgagggat aaagatgccg actcttgatg taacagacgt gctttttgac cccgattttt
    25861 gcgacttcaa cctgtgggta acgcgtcgcg tgcaaacggt ggacgatgac gggatcggca
    25921 gtgacagcga agttaaaacg cagtttgccg gagttgttac cgttgaccgc tccctggaga
    25981 accgccgtat gcagtccggg caagttatca gtggcgcgat tcttatcgtg acaactgagc
    26041 ggctgacaca ggggcagact ggccgtgatg ccgatatcgt gacgtatcag ggccgtgatt
    26101 atcgtgtgac tttcgtcgac ccgtatacag cttatgggtc cggattcgtt caggcgcatt
    26161 gtgagttgct gccgtttgat gggggaattc cggttgagca ataacaccag cacagagcgc
    26221 ggatggctga taccaaccag tggcgatccg gattatgacg aagcgctcga caggctgtta
    26281 agccagtgga tgcgtaacgt ttccggtctg tctgccggga tggttcgccc gcgctggcag
    26341 aaagagcagc cgccactgct accggttgaa acgaactggt gtgcgtttgg ggttatcgga
    26401 tggtcaggtg atgacagtcc ggcattcacc agacagaccg atgatggctc tcagctctgt
    26461 cggcatgaaa cgattgagtg tatggcttcg ttttatggtc cggcggggat ggtgtatgcg
    26521 tcccggtttc gtgacggtat atctgtaccg cagaacaacg cagcactgaa tgcgctgggg
    26581 ctgtctcttg gcgattacac aggtctgact cccttccctg aacttattaa tcagcaatgg
    26641 gtccgccgct acgatatgaa ggtgcgtctg cgccggaagg ttgtgcgcga gtacggtatt
    26701 aaatcgctgg tggaagcacc agtcatcttt ttcggagatt aagctatggc acagggcttg
    26761 cctgtatcaa acgttgttaa tgttgatgtg atcatgtcgc cgcgtgcagc atcagggcga
    26821 aattttggtg cattactcat tctcggcccg tccacaatca ttccggtaag tgagcgcatt
    26881 cgccgttatt ctgccgcgga agatattgga aaagattttg gcgtggaatc accagaatat
    26941 aaagctgcgc aggtgttttt ctcacaatca ccgaaacctc aggaggtttt tgttggtcgt
    27001 tgggtgaaaa cgaagggaga cagcgaacag gccacgcctg agacgctgga gcaggctgtg
    27061 aatgccatgc ttgattatac ttcatggtat gggctgggga ttgcagacga tgcagatatt
    27121 ccggatgcag actggctgaa agtggctgcg gcgatcgaat cctcttctgt aagccgtatt
    27181 ctggcgatta cgacaagcga tgaaaaatgc ctgcagactg catccagcga tgatttggca
    27241 tcaaaactga aaaccgccgg atattcacgc agttttattc agtattcatc gggtaataaa
    27301 tacgctgcgt tatctgcatt tggccgggca ttcacggtta atttcaatgg cagtaatacc
    27361 gcgattacgc tcaagtttaa gcaggagccg ggtgtcgggt atgaaacact gacagtcagc
    27421 caggcatcgg cacttgatgc aaaaaactgc aatgtgttcg tgtactacca gaatgataca
    27481 gctatcctcc agcagggagt gatggctaac ggcgatttct ttgatgaacg ccacggcctg
    27541 gactggttac agaattatgt gcagaccaac ctctataacc tgctttatac cagcaccacg
    27601 aaagttcccc agactgaagc cggtattacc cgactgttat caaatgttga aaaatcactg
    27661 gatcaggccg ttcagaatgg actgattgct ccgggcgtat ggaacggggg cgaccttggc
    27721 cagttgtcat caggtgacac gctgcccaaa ggttattacg tatacgccca gccgctggat
    27781 gaacaggcac aatcagaacg tgaagcccgt aaggctccgg tgattcaggc tgcaataaaa
    27841 cttgcaggcg cggttcatta cgctgacgta cagattaacg ttgttcgcta aggggaagtg
    27901 aatgtctacc tattctttta tggatgtcac tgcgacgctg accgggccga ccggttcgat
    27961 tgacctcggg tacggttctg caagttctga agaggggatt gtggttgcga tgggcggccc
    28021 taaaaacacc atgaccatcg gttctgatgg cgaagtgatg cacagtctcc atgcagataa
    28081 aagcgggacg attaccgtta accttctgaa gacatcaccg acaaataaaa aattgtcgct
    28141 ggcgtataac gcacagagcc agtcttctgc cacatggggg aataacgtta ttgtgatccg
    28201 aaacaaggtc agcggcgaca tcatcacggc acgcagtgtt gcgttccaga aacaaccgga
    28261 taatgccaac gctaaaaccg gtaatacgat gccgtgggtg tttgactgcg gcaagattga
    28321 ccaggttctc ggggagtttt aatacatgga attcgaaatc aaaggcgtga aatatcgcgc
    28381 ggcaaaactc agcgtttttg atcagctgaa agtgacccgc aaacttctgc cggtgctggc
    28441 aggaatgatg tcagatttcg ggagcattcg ctcccgtttg cctgctgatg gcaaaatcga
    28501 caccgtgaaa ttcgagcagt taaaaccggt gtttgaaacc atgctcccgc gtatcgctga
    28561 ggaactgtct tccctgaccg aagatgacac cagtgcgatt attcatccct gtcttgcggt
    28621 ggtatcgcgg cgtcatatgg acggatgggt tccggtattt acccagggcg aactgatgtt
    28681 tgatgatatt gacttgctgg tcatgctgca tctggtggcg cgggtggtcg ccgattcgct
    28741 gggaaatttt ttgcctacac cccttaccag cacgacgcag agcctgcaac agggctgacg
    28801 tttaacagcc tgccggacgg gctgtcttac cttctcaatc cggttgacgc cgggttaatt
    28861 ccttatacag cacttaaaga tggctctgtc gatttgtacg acattgctct cttgaatgac
    28921 catctggcgg taaaagcgga taaccagcgg cgcattgaga aatggagaga ggataatgaa
    28981 cgctgaaact attaaagatt tcctcgtctc gcttggcttc agcgtggatg atgcaggagc
    29041 gaaaaagttc ggttctgtcc tcgccggtac aactgcaaat gtcatcaaaa tgggactggc
    29101 cgttgaagga gctgcgctgt ccgtggtggc cttcacggct aagatcgcct ccggcctgga
    29161 taatctttac cgggcgtcac agcgcaccgg cgcgacagtc cagggaattc agtctattgg
    29221 ctatgcggtt tcgcaggttg gcggcagcgt ggacgcggcg cgaacctctc tggaaagcct
    29281 ctcccggttt gttcgtaaca atcccggcgc ggaaggcttc ctgaatcgcc tgggggtaca
    29341 gacacgggat gccagcggta acatgcgtga catggccgct atttttacag gtgtaggcca
    29401 gaagctcagc ggcatgccgt attaccgggc taaccagtat gcgcagatgc taggcattga
    29461 cgaaaatacc cttatggcga tgcgccgggg tgtgggtgat ttctccgggc agtacagcgc
    29521 aatggcgaaa gctatcggct tcaatgctga cgaggcggcc agaagctcca acaaattcat
    29581 gacctccctg cgcgagttcg gcgcgatggc aggcatggcc cgtgacaaaa tcggctctaa
    29641 tcttgctggt ggtctggcgg gttcgctgga cacactgcgc cgccacatcc tcgataactt
    29701 cccgcgcatc gagcagaccc tgacgaaagc cataaaaggc attctggcga tcggagacat
    29761 catcgggcgg ctgttcttca ggctaattga gggaacatcc agccttatca cctggtggca
    29821 atcgctggat aagcaaacgc gggagctcat ctcgctgttt ggcgcgctga cgattgcgct
    29881 gcgcattctg aacagtacgt tctggatgtc gccgattggc ctcattaccg cgctggcggc
    29941 ggggattgcc cttctgtggg aggactatca gacctggaag gaaggcggcg acagcctgat
    30001 tgactggggc aagtggaagc cggaggtcga tgccgcgctg aagatggttc gtgacctgaa
    30061 agggtctgtt aatgaactgg cgaaagcgct ggcgaaactg ctcaatattg accccaaatc
    30121 atggtccctg aagtgggatt tcagcaactt catcgaccag atgggcgaat tcagcaaaat
    30181 gctgaacatg atcgccgacc tgctcaacgc tatcaaagat ggccgctggg ctgatgccgt
    30241 catcatcggc aaacagatac ttaatcaggg cagcgaaaat ccgtcagcga tgccgatggt
    30301 tacagacagc gctaacagta ctgccgactg gattaaagag cactggggat tcgatccccg
    30361 cagtgtgggc cggacggtac gcggctggtt tggtgatgat gagccggaac aatatgcaca
    30421 ggctacgaaa cgaggagaac ggaataacaa tccgggaaac cttaattttg ctggtcaggc
    30481 aggggcttct cttgaacgcc cgggcgggcg atttgccaga tttgaaactg cttttgatgg
    30541 attacgggct cttgctcgtc agttaatgct gtacgccgga cggggaataa acagtgtgga
    30601 gaaaattatc tctacctggg cacctgcgtc tgataataac aacacaactg cgtatatcag
    30661 ggctgtatcg caacgactgg gagtggatcc ccgggctgcc ctgaatatga gcgatccgca
    30721 aaccatgtca gcattgatga gcagcattat ccagcatgag aatggaagaa atatctattc
    30781 tcgagagctg attaataagg ctgccgtggc gggaattagt ggcaaaatga cagaggttaa
    30841 ccagcaaaat acttaccaca tttacggtgg cggagatccg cacgctgtcg gtaatgaggt
    30901 tgcacgtcgg caacagtctg caaatgctca ggtcatgcga agtaatcagg tgagggtggg
    30961 ttagtggata ttctctctac actttttcat cagcagagca gaaaaatagg aatgattgtt
    31021 cccagtgttg ttatttcaga gaagcataca gatatgcttg aaataacaga gcatccggta
    31081 gaggtcgggg ccgctgtcgc tgatcatgcc tataaaaaac cgtcagaagt ggtgatggag
    31141 gttggtttcg ccggtggcgg cgcattgctg gattttgcca gtaatctgac ggctaccagc
    31201 ctgctcggcc tgagtcctca gcagacgtat caggagctac tggatctgca ggaaagccgt
    31261 atccccttcg atgtggtaac cggtaaacgg ctgtacagca acatgttgat ccgggcgctg
    31321 gaagtgacga cggacaagac aaccgaaaac gtcctgtccg ccgtcctcac cctgagggag
    31381 gtcattatct cccggacaca gcagattacc gtcgcggata aaaccaacat gaaggaaggg
    31441 gccagcacgt cggcggtaca gaatagcggt aacaaaacca caaagcctcc agatacttca
    31501 ctgctgaaaa gcatcacggg taacgtggcg tcattactgg gaggcggcta atgacaattc
    31561 aggaaattcc gctgacagcg gacaaccagc agttcagcat cgtcctgggt ggtgtcacct
    31621 ggcggattag catcatatgg cgcgatcctt actggattat ggacctgcag aacgacagag
    31681 gagagccggt aatctccggt attcctctcg tcactggcgc tgatctgctg gcgcagtacg
    31741 cctgtatggg acttggtttt aagctgttgg tggtctgcga tgacaacaca caggattatc
    31801 ccacgaaaac tgacctgggc gggcgcagtc atttgctggt atcaacggag taagcatgtc
    31861 acagaactgg atgagacatt tcgagctgca gcttgtggac gggaacggta agggaattga
    31921 gctaagtgat ttcaaagtca cctttacgat cgactggttc aacatcagca gcgcgtcccg
    31981 ggtagggact atcaaaattt ataacctttc ggcagatact gtgaaccgaa ttaccgggca
    32041 ggagttttcg aaagtgcggc tgattgccgg ttacgacggt atcgcgccgg aggtggcggc
    32101 aagcgacgtc gggactgtgc gtgaagttga cgcggcggac gtgggccaga gtgatggccg
    32161 caactacggg ctgattttca gcggagaaat tcgctactcg gtcacaggaa aagacagtcc
    32221 ggttgatacc tacgtcctga ttcaggcagc agatactgat ctggcatttg ccaccagtat
    32281 aacctcacag acgctggctg ccggttacac agtcgcagat gtgaaccgtg cgctgatgaa
    32341 agactttgag gccaaaggcg cgaccgaagg actgacgcct gaaatgcctg ctaccgtata
    32401 ccctcgaggg cgggtgctgt tcggcatgac acggcatctt atggataacg tggccggaca
    32461 atgtggcgca acatggcaat ttgtggacgg tcagcgccag atggtggcga ataacgagta
    32521 tgttcacgac gcgattgtgc tcaacagcgc caccgggctt atcggcatgc cgcagcagac
    32581 tatcggtaac ggcgtaaacg tccgcgctct tattaatccg aacatccggg ttaacgggct
    32641 tattcagctg gatcaggctt ccgtgtaccg caccgcgctg tcgaacaatg atatcgcgat
    32701 ggctggtggg cagatcaccg accagaacac agacggaaat atcacgctca gcggcaccac
    32761 agcacaacct gccagcatcg caacggatgg cgtttatatt gtgcgcggga ttatgtacac
    32821 tggcgataca aggggccagg cgtggtacat ggatatgatg tgcgaagcgc gtggcgcggc
    32881 ggatctgtat acgcaatcgg ctttgcaaag gtgatgagca atgaggggta tcatttttct
    32941 gttagctgtc ttttctgcgt gcagcgtgtg ggcggatggc ttcacggtta aatgcggtgg
    33001 ctacactatg gttgcaaacc agggcgagtt atcgacaatt aacggtgaaa gagttacctc
    33061 tcaaaaaatc accgaactgg gtaccaatgg tttgaaagta gacatgggga ttatgcctgc
    33121 caaagacggt aacaactacg gctttgaata cattcgtcgc cctggtaccg aaacgcgatt
    33181 cctgaatgtg caactgctgc agaacagcat ggatgcaccg aaaatcatcg gatctttccc
    33241 gtgtaaaaag gttgatggtt aacccaaagt tacatttctg catagtgttg agtttgttca
    33301 cattcactaa ataatgattt tttatcgctt gctaggtggt tgcatgatca ctaaactttg
    33361 cgaactttta gcgctcagct aactttgaat aagtggtgta tagtcgttta aaacgacaga
    33421 gggattgggt atggcgatta gctacgcatt tgcgctggca acaattacac aacaaatgaa
    33481 tcaggttcag gaagctgtta acggtgcttt caaacccctg atctctaatg cttgtgaact
    33541 gccacaacga ttagatgctg aagaggtatt tcgtcgttgt accgcgattg ctgcacgcgc
    33601 tcaagaaatc gaaaataccg cgaaggaagg catgtctcat ctcgaagctt ttcgaaatgg
    33661 gagaatcatt gttgatgagc ttccggaaga gtttttatcc catctcgaag gtcttgccaa
    33721 ggcatgccga aatgctaaag gacatttagt ggatatgttc tctgaagcgg aaaggtcccc
    33781 tatgtggcaa ggccatctgc aaatgttgcg cccattaaaa cgcaaatatg ttcgtgcgtt
    33841 gaccgccgtc gagaacactg caactcagtt ggccgctgaa gttaggcaat cgcagccttt
    33901 ccaggctgaa ctcttgtcag ataatgttac ccgtgaagag gctattgaac tgatctcaac
    33961 atcgcataag atgctgggag ccaacgccca taaatggatg tgacatggca aaagtcagta
    34021 tcacgggaga attgcgtcat ttagctgcag cacataagta tgctcaaatg cttgccgatt
    34081 atatttcaaa gggttcccag ttctggtgtt ttggttcgtt aggcggtttt gaacgtaact
    34141 atgatgcgat ggctgccaac atcaggaaaa ttcacttaaa gctacctggc gataaaccct
    34201 ggcctccaga agcatctctg agtgaacgga catgtgataa ttttttggta tatgctcagc
    34261 atctttatat cgatgaacac tatcagatat tggcaatcat cagcccaaac gctcatcagc
    34321 aagctgattc gatgcttccc cggctgataa aattagcaga ggaaaccttc atagaacttc
    34381 ctcctgatga actcgaaaaa ttgaaaacct acgattcata aacccgccac ccggcgggtt
    34441 ttttgctttc tggagcctac taaatggcag tatctgacca gacccgcagc ggcgaccttg
    34501 ccgaaacatt caaatctgaa cgggaaacaa caaagaatca gatccgtgtc gccttgcctg
    34561 gcattattca gtcattcgat cctgatgcgg tgacggcggt tgtgcagcct gctatccgtt
    34621 cggttgaaaa ggataatgac ggcaaccgca ttaccaaaaa ttacccattg ttggtggatg
    34681 ttccagtggt attcccacgt ggcggaggat gtacgctaac gtttccgatt aaagccgggg
    34741 atgagtgtct tgtcgttttt gccgatcgtt gtattgattt ctggtggcag agcggcggaa
    34801 tacaggaacc ggtcgatgac agaatgcatg atttatcgga tgcgttttgt attgtaggcc
    34861 cccagtcgca ggcgaggaag attagcggca ttaataccag tgccacacag ttgcgtagtg
    34921 acgatggcag cacctatttt gagcttaatc ctgataccag gaaaattaaa attgtcgctc
    34981 cgggtggtct tgatgtggtt gcccctctgg ctgatttttc tgagaaagta accattcatg
    35041 gcctgttaac ctggatgggg ggcatggtgg ggtctgttgt ttctggtgtg gcttcaaaaa
    35101 tcactggtgt tgttgagttt ttgggtagcg tgaaggctaa cggcaagcca atcgatgata
    35161 cgcacactca tggcggtgtt cagcgcggtg gaagcaatac cgatggggta aactgatgcg
    35221 atacagacgt gaagacgccg atggcgatta cacctttggc agcggtgatg acacctggct
    35281 gattaactca ccggaggccg tggcgcaggc ggtaaaaacg cgattcgaat tgtggtatgg
    35341 gcaatggttt ctcgatacca ccgaagggac tccgtggatc cagtccgtac tcggtaagca
    35401 gaagccggaa acctacaacc tggcgatccg taagcgcatc ctcgaaacgc ggggcgttaa
    35461 atcaatcctc tctttcaata cgacggtgga taccacgacc cgacgtgtca tgttttccgc
    35521 tgaaatcgac actctttatg gaataacgac tgttacatcg gaggcgtaat ggctctgaac
    35581 cttgattctc ttggtttatc tgcaaaggta accgcagagg ggatcagtgc gcctgattat
    35641 cagacgatac tcagcaccct gattagctat tttcagcaga tttatggcag tgatgcctac
    35701 ctcgaaccgg acagcaaaga cggccagatg gtggctctga tggcgctggc gattcatgat
    35761 gccaataata tggcgataac tgtctacaac tgtttttcac cggcaaccgg ctatggggct
    35821 gcactgacca gcaacgtgaa aataaatggt atttcacgta aaggcgcgac gaactctacg
    35881 gttgatttgc ttcttacagg aactgcagga acaaccatca ttaatggcag cgtgaaagac
    35941 agtaataatg tgatatggcg tttgcctgct tcagtggtgg tcggcgtgga tggtacagtg
    36001 atggcgaccg caacatgttc cgtcagtggt gcagtggcgg cgctggctgg aactatcact
    36061 gaaattaata cgccaacccg tggctgggtt tcggtaacta atcctgctgc ggctactgtt
    36121 ggctctccgg cagaaactga tgctgagtta cgtatccgcc agtcgcaaag tgttgcgttg
    36181 ccatcgataa ccccatttga agcactggat ggtgctgttt ctaatgttac cggtgtaacc
    36241 cgccacaaac tctatgaaaa cgatactggt tcggaggacg gtaacgggtt accgccacac
    36301 tctgttgctg taattgtgga tggcggtgat gtgacggata ttgctcaggc tatcagagga
    36361 aataaaggcc aggggacagc cactcacggt acaacatccg ttacggttcc ggataaatac
    36421 ggcaatcccc atgtaatcaa attctcgcgt tccagtgatg tgcctgttta tgcccggatt
    36481 aaattaaaag tttttacggg ttatacctca cagatagggc agcagatcca gcaggctatt
    36541 tccgactata tcaatagtct gacgattggt gattcagtcc ttttaagtcg catttactca
    36601 ccggcgaatc ttggcgtggt gagtggcggg aatgcacgct attacgatat tcaggaactg
    36661 acgattggga aatccccggg ggctttgtcg tcatcaaaca ttgatatcag atacaacgaa
    36721 tctgcgtcct gtaccccaga aaatatcgtt ataacggtgg agtcatgagc aaatacaccg
    36781 aactaatcac gaactaccac gccaccaaac ctaaatttct tgcgcatgtt gatctgatga
    36841 cccggccact tattgatgtt gcggctgcca ccagagggct gattactgca tttgatattg
    36901 actctgcggt tggtgtgcaa cttgacattc ttggattgtg gatcggacgc agccgtgttg
    36961 tcagccagcc tatctcaggt gtctatttca gctgggatac cgacgggctt ggatatgatc
    37021 agggtgtatg gcaggggcca tacgatcctg attccggata catgtacctc agcgatgaaa
    37081 cttatcgtgt catccttaaa gcgaagattg cgattaataa ctgggacgga cggaatgatt
    37141 cgcttccggc aattcttgac gcggcgacag caggatccgg actgcgaatg cagatagtcg
    37201 ataaccagga catgacgata tcggtctggc tctttcctga tactgatatt tcagatgtat
    37261 cgcgtgagtt aattgcggca attaaacagg gatatctcac agtaaaagcc gccggggttt
    37321 gggcgggtgg cattgaaaca ccttcggtgg aaactccatc ggaaggttca aaattttttg
    37381 gttttgatat ggataacgaa ttcatcagtg gttttgatgt aggggcatgg ggagtattac
    37441 tctgatggcg aaaaatgact ttaaagcgtt tgcaactgat cggaatgcca atgttatgtc
    37501 gcaggaggaa tgggaagcgt tgcctgcgct tttatccgga tttacagcag ggaaagcatc
    37561 cagtgcgcaa gtcaataagg ttattcggca ggccagcttt attgctgcag ctctggccca
    37621 gtttgtaagt gacaaaacgc aacgggatgt gcttgataat ggtgatctgc ccggttttgt
    37681 tgaattgctg ggatcggggt ttgctgttga atacctgagc cgcaagaatc cgtttggcga
    37741 tatcaaatcg gatggcacgg tgaaaacggc tctcgaaaac cttggtttag gagatatcag
    37801 tgctacaggt gttgcgacag gtagtatggg cgaaaccgga tatgccatat ttccgatgat
    37861 gattggcggt acgcgtaaga cgcttattat gcaatgggga gtaatgacat ctctgagatc
    37921 aggcggcact atggatatta atctgccgat tgcttttcct actatggtta tgcaggcata
    37981 tgcaacacat gataatgtga tacaaaccgc acgaccgaca gtatatgcaa ttgccccaac
    38041 aagccgaacc actatcactg cgacggcgat gagcttacgt gctgactcag tgggtgctgc
    38101 agtgacgcgc agtagtgaca gtgatttgtg ttacggccgt tatcttgcaa tagggtactg
    38161 aaatgaatct gaaatatttt tctccatcag aaaatgcgtt ttatgccggg gagcttaagg
    38221 aaagctatat tgctgcagac tcatggcctt ctgatgcctt ggaggttgac gaaagtcttt
    38281 atcttgagtt tatcgatgag ccgccagcag gtaagtatcg tggagtggat gatgcaggta
    38341 tgccctgctg gcttgaaatt cctccaccca ccgcagagga aatggcatcg aatgcagaga
    38401 atatgagaca gcagctcatt gatgaggcaa atgtttatat aaacaaacgt cagtggccgg
    38461 gaaaagcagc gataggccgt ttaaaggcgg atgaactgga acgatataac ctttggctgg
    38521 attacctgga tgcgctggaa gtagtagaca cctccagcgc gccaaacatt aactggcctg
    38581 atcctccgga tgcgtaggcc atacgggttt tgctgtatcg acacgcatta gcaagacccg
    38641 atatttcttc cattcggcca gagcgacggt ttcctcatcc gtcgcgatac caacatcaac
    38701 tgcatcctgc cgccaggcta tctcagcgtc agcaatagcg cgtaaactgc tgggataaca
    38761 gcccaatgga acgcttcttc aggagtctga agaacgaatg gatgccggtg gtgggttacg
    38821 taagcttcag cgaggcagct cacgccataa cggactatat cgttggatat tacagcgcac
    38881 taagaccgca cgaatataac ggtgggttac ccccaaacga atcggaaaat cgatactgga
    38941 aaaactctaa ctcggtggcc agtttttgtt gaccacttca cacgtcgtat gcaagaacgt
    39001 gctgcggctg gctggcgaac tttcgatagt gcgagtattg aatgatttcc aactgttgcc
    39061 gattttacta tgttttcagt agaacactta gacaaaactg aggcacacaa atctttgcac
    39121 tggattgcaa ggctttgtgc tcttcgatag tggttaagga ggattactcc acctttccat
    39181 caagccaatc cgcccaccac tgcatcattt ctctgcgctt atcgagatac tgagcatggt
    39241 tgtaaattcc acgcacagaa ccgccgttgg catgtgccag ttgcacttca atagcgtcag
    39301 caggccattc gtgctcgttc ataatcgtgc tgaattcatg cctgaatccg tgaccgcttt
    39361 ccagaccttc atagccgatt tgtttgatca caagcaatac cgcgttctcg cagattggct
    39421 tcttcttatc gttgcgcccg gcaaaaacaa actctgagac tggtttggtg attgagctta
    39481 gcgtagtgag aagttcaacc acctggtctg acatcgggac cacatgaatt ttgcgtccct
    39541 tcatcacact ggcgtcgatg gtgataatcc tgttttcaaa atcgacgttc ttccatagca
    39601 tggaacgaag ctctttcgtt cttagggctg tataacgtaa aacttttgtc gcaatgagcg
    39661 atacgatact tcctgaaaat gttgccagtg ctttgttgaa tgcaggcatc tggtctgctg
    39721 gaaggaacgg gaagttcttc ttgcggtatc ctttcatggc gtctgcaagg tcaggtgctg
    39781 ggttatattt agcccttccg gtgacaatag cgtaacgaaa aacctcgccg catcttctgc
    39841 gggctttgtt ggctcgctcc attgcaccgc gatcttcaaa tctgcggatt acttccagca
    39901 gttgcatcgg ctcaatatcc tgaatctcaa gaccgccgat gatgggtaaa atgtcgtcat
    39961 caaacatttt ggcaagttca gttgcatagc ctactgacca gacttgcttc ttgtgctcgt
    40021 accattcctt gtaaatcgca ctaaatgaat tgttgttaga cgaagccttt ttcgccttta
    40081 ccggatcgat gccaaccgag atgtctttcc tcgcagtcca tgccttatcc cttgcttcct
    40141 gcaaagtcat aagcggatat tttccgacag tcaggatttt ctccttaccg tcaatcttgt
    40201 agcgaagctg ccataccttt ttcccggata cagggacata aaggtacagg ccattaccat
    40261 cgagaaggcg gtatggtttt tctttcggct ttgctgcttc aatctgctta acggtgagca
    40321 tgggtaaaaa tccggtgggt aaaattattt tatccacttt ttacccgtca tggagtgcgg
    40381 ctgtcaacga tctgacgcga accatgacga accgtgaata tacggaagga ttgatattca
    40441 ggggattttg cggactagta cggatggtgg cgaactgata aatggtgtcc cctgcaggaa
    40501 tcgaacctgc aattagccct taggaggggc tcgttatatc catttaacta agaggacaat
    40561 gcggcatgag tatacccgct aatggactgc ggggtaagta cgctgccgct cgattgctta
    40621 aaccctcgcc atttatgctg ggtttttatc atttttctta atgttttccg cacgttctgc
    40681 tttttggcgt gcttctgctt tacgcttgtt gctcatgtcg ttacgaatct gtgcatgact
    40741 cattaacgcg aagataaagg tgccgccgca gatgttcccc gctaaagtag gtagtgcgaa
    40801 gggccagatg aaatcgctcc agtgcagcgt gccgttaaac accagataga ggatttcaac
    40861 agaaccgaca acgatgtggg tggtgtcacc cagtgcaata agccaggtca tcaatataat
    40921 caccacaatc tttgccgcac ccgcagcagg aaacatccaa accatagtgg cgatcagcca
    40981 gccggaaatg atcgcgttgg caaacatctc gctgggggtg ttcttcatca catccatgcc
    41041 gattttgaca aatgcatcgc gagtttcttc attgaagata ggcatatatt caaatgccca
    41101 tgcagcaata cctgtcccga gaatattacc cagcagcacg acgccccata atcgcataag
    41161 taagccgacg ttgctcattg tcggtttttg catgacgggt agtaccgcag tcacggtgtt
    41221 ttcggtaaat aattgctggc gggccataat gacgataata aaaccaaagg tataaccgag
    41281 attctccagt aagaagctgc ctggcactcc ttccagttcg acatgaaata tcccttttgc
    41341 cagtagcgaa gcgcccatcg acagacccgc cgcaatggct gaccacagta gcgccattgc
    41401 gtcgcgttcc agctcttttt caccatcctg gcggatatgc tcatgaattg ccatcgcccg
    41461 ggaggggagt cggtcttcat ctatttctat ttttttgccg cgctcttttt cttcgctctc
    41521 aacttcaatt tcgtcgctgt gttgatcaat tttgtcgttg tccatggtct cttctcttga
    41581 attagcacgt atagctaaag cgtagcggct tttttgctcg caactggcgg gagttactct
    41641 gaaaatgtag aaaaggctgc gtttgccttt ttctgtttct atagaatcaa gtagcctaca
    41701 gggcggcgat taccaggcta tgatcaaatc agcaaatcag ggcgtctgga catcagttga
    41761 cgtgctgtta caatcgccca cacctaaaca ggcggatacg gtatcgttcc gtcatggatg
    41821 gcaaactgca taagccataa aaaaacaggg agacatttat gaagcttcgc ctgtcggcgc
    41881 ttgctctggg aactacgctt ctggtggggt gtgcgagttc cggtacagat cagcaagggc
    41941 gttctgaccc gttagaaggg ttcaaccgca ccatgtacaa cttcaacttc aatgtattag
    42001 acccgtatat tgttcgaccg gtcgctgtcg cctggcgtga ttatgttccg caaccggcgc
    42061 gtaacggttt gagcaacttt actggcaacc ttgaagaacc tgcggtgatg gttaactact
    42121 tcttgcaggg cgacccttat caggggatgg tccactttac ccgctttttc ctgaacacca
    42181 ttttggggat gggcggtttt attgatgttg cagggatggc gaacccgaaa ctgcaacgga
    42241 ctgaacctca ccgcttcggt agtacgcttg gtcattatgg cgtgggttat gggccttacg
    42301 tccagttacc gttctacggt agcttcacgc tgcgtgatga cggtggtgat atggcggatg
    42361 gtctttaccc ggttctttcc tggctgacct ggccgatgtc tgtgggtaaa tggacgcttg
    42421 aagggataga aactcgtgcg cagttgttgg attccgatgg tctgctgcgt cagtcgtccg
    42481 atccttatat tatggtgcgc gaagcgtact tccagcgtca tgatttcatc gctaatggcg
    42541 gcgaactcaa accgcaggaa aatccgaacg cgcaagcgat tcaggatgat ttaaaagata
    42601 tcgattctga ataagaaaca aataaaaaag gtgagtcgca atactcacct tttttgttgt
    42661 tatccctcaa ttaccacaag taaaaggaga taaaccgaca atgacccgga cgtaatgatg
    42721 taaaaatact gatgttcgtc actgactatt tccgatattc agg
//
LOCUS       DAPHDW010000025        41612 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.025, whole genome shotgun sequence.
ACCESSION   DAPHDW010000025 DAPHDW010000000
VERSION     DAPHDW010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 41612)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41612)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41612
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.025"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(147..3296)
                     /locus_tag="RH057_001334"
     CDS             complement(147..3296)
                     /locus_tag="RH057_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV3127730.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWFNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSIPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(3319..4512)
                     /gene="acrA"
                     /locus_tag="RH057_001335"
     CDS             complement(3319..4512)
                     /gene="acrA"
                     /locus_tag="RH057_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HDV3127731.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQEYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATALATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            4654..5301
                     /gene="acrR"
                     /locus_tag="RH057_001336"
     CDS             4654..5301
                     /gene="acrR"
                     /locus_tag="RH057_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HDV3127732.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            5429..8791
                     /gene="mscK"
                     /locus_tag="RH057_001337"
     CDS             5429..8791
                     /gene="mscK"
                     /locus_tag="RH057_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HDV3127733.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(9003..9164)
                     /gene="rsmS"
                     /locus_tag="RH057_001338"
     CDS             complement(9003..9164)
                     /gene="rsmS"
                     /locus_tag="RH057_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HDV3127734.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTRDDEAEK"
     gene            complement(9178..9705)
                     /gene="priC"
                     /locus_tag="RH057_001339"
     CDS             complement(9178..9705)
                     /gene="priC"
                     /locus_tag="RH057_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HDV3127735.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVAQFATLSARFDRHLFQTRAT
                     TLQACLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKIARWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            9775..10152
                     /locus_tag="RH057_001340"
     CDS             9775..10152
                     /locus_tag="RH057_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HDV3127736.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPRVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            10305..10856
                     /gene="apt"
                     /locus_tag="RH057_001341"
     CDS             10305..10856
                     /gene="apt"
                     /locus_tag="RH057_001341"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HDV3127737.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISETYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            10985..12916
                     /gene="dnaX"
                     /locus_tag="RH057_001342"
     CDS             10985..12916
                     /gene="dnaX"
                     /locus_tag="RH057_001342"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HDV3127738.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEANGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTQVPPQ
                     PQSAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERL
                     ASVTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPE
                     LAAKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNR
                     GAQQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNI
                     QTLRRFFDAELDEESIRPI"
     misc_feature    12249..12313
                     /gene="dnaX"
                     /locus_tag="RH057_001342"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            12969..13298
                     /locus_tag="RH057_001343"
     CDS             12969..13298
                     /locus_tag="RH057_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HDV3127739.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            13298..13903
                     /gene="recR"
                     /locus_tag="RH057_001344"
     CDS             13298..13903
                     /gene="recR"
                     /locus_tag="RH057_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HDV3127740.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            14013..15887
                     /gene="htpG"
                     /locus_tag="RH057_001345"
     CDS             14013..15887
                     /gene="htpG"
                     /locus_tag="RH057_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HDV3127741.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWEKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTHTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            16068..16712
                     /gene="adk"
                     /locus_tag="RH057_001346"
     CDS             16068..16712
                     /gene="adk"
                     /locus_tag="RH057_001346"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HDV3127742.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDVGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKPVAEVRADLEKILG"
     gene            16948..17910
                     /gene="hemH"
                     /locus_tag="RH057_001347"
     CDS             16948..17910
                     /gene="hemH"
                     /locus_tag="RH057_001347"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HDV3127743.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESAVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMAPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(17907..18866)
                     /gene="aes"
                     /locus_tag="RH057_001348"
     CDS             complement(17907..18866)
                     /gene="aes"
                     /locus_tag="RH057_001348"
                     /EC_number="3.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
                     /protein_id="HDV3127744.1"
                     /translation="MKPENKLPVLDLISAEMKTVVNTLQSDLPSWPATGTIAEQRQYY
                     TLERRFWNAGAPEMATRAYMVPTKYGQVETRLFCPQPDSPATLFYLHGGGFILGNLDT
                     HDRIMRLLASYSQCTVIGIDYPLSPEARFPQAIEEIVAACCYFHQQAEDYQINMSRIG
                     FAGDSAGAMLALASALWLRDKQIDCGKIAGVLLWYGLYGLRDSVTRRLLGGVWDGLTQ
                     QDLQMYEEAYLSNDADRESPYYCLFNNDLTREVPPCFIAGAEFDPLLDDSRLLYQTLA
                     AHQQPCEFKLYPGTLHAFLHYSRMMKTADEALRDGAQFFTAQL"
     gene            19018..20322
                     /gene="gsk"
                     /locus_tag="RH057_001349"
     CDS             19018..20322
                     /gene="gsk"
                     /locus_tag="RH057_001349"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HDV3127745.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEALYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVDLVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSLEESYWDR"
     gene            complement(20455..22131)
                     /locus_tag="RH057_001350"
     CDS             complement(20455..22131)
                     /locus_tag="RH057_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HDV3127746.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKAIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSLAAFFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(22369..23589)
                     /gene="fsr"
                     /locus_tag="RH057_001351"
     CDS             complement(22369..23589)
                     /gene="fsr"
                     /locus_tag="RH057_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HDV3127747.1"
                     /translation="MAMSEQPQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIAPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYASLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            23807..25459
                     /gene="ushA"
                     /locus_tag="RH057_001352"
     CDS             23807..25459
                     /gene="ushA"
                     /locus_tag="RH057_001352"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HDV3127748.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNPEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYHLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGETNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(25496..25975)
                     /gene="ybaK"
                     /locus_tag="RH057_001353"
     CDS             complement(25496..25975)
                     /gene="ybaK"
                     /locus_tag="RH057_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HDV3127749.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMAAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(26179..26973)
                     /locus_tag="RH057_001354"
     CDS             complement(26179..26973)
                     /locus_tag="RH057_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HDV3127750.1"
                     /translation="MDLLYRVKTLWAALRGNHYSWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            27111..27452
                     /locus_tag="RH057_001355"
     CDS             27111..27452
                     /locus_tag="RH057_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV3127751.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(27566..30070)
                     /gene="copA"
                     /locus_tag="RH057_001356"
     CDS             complement(27566..30070)
                     /gene="copA"
                     /locus_tag="RH057_001356"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HDV3127752.1"
                     /translation="MSQTIDLTLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMMVTADNRSL
                     WLVIGLITLAVMVFAGGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGKSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAVFVPVVVVIALI
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNEQQVGTKAIEAEITA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            30332..31264
                     /gene="glsA"
                     /locus_tag="RH057_001357"
     CDS             30332..31264
                     /gene="glsA"
                     /locus_tag="RH057_001357"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HDV3127753.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGKVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENVEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLLNTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            31267..32559
                     /locus_tag="RH057_001358"
     CDS             31267..32559
                     /locus_tag="RH057_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3127754.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWVAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPRSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASAINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLGSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGSLLLSLIVAMVMKRNKTV"
     gene            32684..33091
                     /gene="cueR"
                     /locus_tag="RH057_001359"
     CDS             32684..33091
                     /gene="cueR"
                     /locus_tag="RH057_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HDV3127755.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(33092..33550)
                     /locus_tag="RH057_001360"
     CDS             complement(33092..33550)
                     /locus_tag="RH057_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HDV3127756.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(33547..34464)
                     /locus_tag="RH057_001361"
     CDS             complement(33547..34464)
                     /locus_tag="RH057_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HDV3127757.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            34610..35287
                     /gene="fetA"
                     /locus_tag="RH057_001362"
     CDS             34610..35287
                     /gene="fetA"
                     /locus_tag="RH057_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HDV3127758.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRNQQPDPAIFLDFLERFALPDSILTKDIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            35274..36053
                     /gene="fetB"
                     /locus_tag="RH057_001363"
     CDS             35274..36053
                     /gene="fetB"
                     /locus_tag="RH057_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HDV3127759.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFVAITVG
                     AGITLAVLILSGSIEFIPMQVIPIAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(36116..36970)
                     /gene="cnoX"
                     /locus_tag="RH057_001364"
     CDS             complement(36116..36970)
                     /gene="cnoX"
                     /locus_tag="RH057_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HDV3127760.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADTPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(37031..37840)
                     /gene="ybbO"
                     /locus_tag="RH057_001365"
     CDS             complement(37031..37840)
                     /gene="ybbO"
                     /locus_tag="RH057_001365"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV3127761.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPDDVERMNSMGFTGVLIDLDSPESVDRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLKRLLPGRVMDKILQG"
     gene            complement(37830..38453)
                     /gene="tesA"
                     /locus_tag="RH057_001366"
     CDS             complement(37830..38453)
                     /gene="tesA"
                     /locus_tag="RH057_001366"
                     /EC_number="3.1.1.5"
                     /EC_number="3.1.2.14"
                     /EC_number="3.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
                     /protein_id="HDV3127762.1"
                     /translation="MNFNNVFRWHLPFLFLVLLTFRAAAADTLLILGDSLSAGYRMSA
                     SAAWPALLNDKWQSKTSVVNASISGDTSQQGLARLPALLKQHQPRWVLVELGGNDGLR
                     GFQPQQTEQTLRQILQDVKAANAEPLLMQIRLPANYGRRYNEAFSAIYPKLAKEFDVP
                     LLPFFMEEVYLKPQWMQDDGIHPNRDAQPFIADWMAKQLQPLVNHDS"
     gene            38424..39110
                     /locus_tag="RH057_001367"
     CDS             38424..39110
                     /locus_tag="RH057_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3127763.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            39107..41521
                     /locus_tag="RH057_001368"
     CDS             39107..41521
                     /locus_tag="RH057_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3127764.1"
                     /translation="MIARWFWREWRSPSLLIVWLALSLAVACVLALGNISDRMEKGLS
                     QQSREFMAGDRALRSSREVPQAWLEEAQKRGLKVGKQLTFATMTFAGDTPQLANVKAV
                     DDIYPMYGDLQTNPPGLKPQAGSVLLAPRLMALLNLKTGDTIDVGDATLRIAGEVIQE
                     PDSGFNPFQMAPRLMMNLADVDKTGAVQPGSRVTWRYKFGGNENQLDGYEKWLLPQLK
                     PEQRWYGLEQDEGALGRSMERSQQFLLLSALLTLLLAVAAVAVAMNHYCRSRYDLVAI
                     LKTLGAGRAQLRKLIVGQWLMVLTLSAVTGGAIGLLFENVLMVLLKPVLPAALPPASL
                     WPWLWALGTMTVISLLVGLRPYRLLLATQPLRVLRNDVVANVWPLKFYLPIVSVVVVL
                     LLAGLMGGSMLLWAVLAGAVVLALLCGVLGWMLLNVLRRMTLKSLPLRLAVSRLLRQP
                     WSTLSQLSAFSLSFMLLALLLVLRGDLLDRWQQQLPPESPNYFLINIATEQVAPLKAF
                     LAEHHIVPESFYPVVRARLTAINDKPTEGNEDEALNRELNLTWQNTRPDHNPIVAGNW
                     PPKADEVSMEEGLAKRLNVALGDTVTFMGDTQEFRAKVTSLRKVDWESLRPNFYFIFP
                     EGALDGQPQSWLTSFRWENGNGMLTQLNRQFPTISLLDIGAILKQVGQVLEQVSRALE
                     VMVVLVTACGMLLLLAQVQVGMRQRHQELVVWRTLGAGKKLLRTTLWCEFAMLGFVSG
                     LVAAIGAETALAVLQAKVFDFPWEPDWRLWIVLPCSGALLLSLFGGWLGARLVKGKAL
                     FRQFAG"
BASE COUNT         9917 a        10948 c        11099 g         9648 t
ORIGIN      
        1 aacaatcagt attttttatg aattttatcc gtggttaata ctggttttcg tatgagatcc
       61 tgagttggtg gttcaattac tccttaatgt tcgtaggtta tgcataaaaa aggccgcttg
      121 cgcggcctta gtgattacac gttgtatcaa tgatgatcga cagtatggct gtgctcgata
      181 tcttcattct tgcggctaaa gcggcggcga accacaacaa agaataccgg aacgaagaag
      241 attgccagta ccgttgcggt caccatcccg cccattacac cggtacctac tgcgttctgc
      301 gcgccggaac cagcaccagt actgataacc agcggcataa cgccgaggat aaacgccagc
      361 gaggtcatca ggatcggacg taaacgcatc cgcactgcat caagcgtcgc ttcaatcaga
      421 cctttacctt ctttatccat caagtctttg gcgaattcga cgataagtat cgcgttcttc
      481 gccgacaacc caatggttgt gagcaggcct acctggaagt aaacgtcatt ggtcaggcca
      541 cggaaggtgg cagccagcaa cgcaccgata acccccagcg gaacgaccag cataacggag
      601 aacggaatcg accagctctc gtacagcgcc gccagacaca ggaatacgac aatcaacgaa
      661 atcgcgtaca gtgaaggtgc ctggttgccg gagaggcgtt cctgatagga catccccgtc
      721 cagtcatagc caacaccggt aggcagtttg ctcgccagtt gttccattag ctccattgct
      781 tcaccggtac ttttacccgg tgccgcctgg cctaagattt ccatggatgg caggccattg
      841 taacgttcca gacgcggcga accgtactcc caacgagaag aggagaatgc cgagaacggc
      901 accatctgac catcagcagc acgaacatac cagtcgccga tatcatccgg cagcatacgg
      961 tatttcgctt ctgacatgac gtaaactttc ttcacacgac cgcggtcgat aaagtcgttc
     1021 acatagctac cgccccatgc agcgcccaga gtggtgttaa tgtcgttgat agaaacaccc
     1081 agcgcctgcg ctttttcctg gtcgatatca atcttaaact gcggggtatc ttccagaccg
     1141 tttggacgta cgctggtcag catatcaggg tgcttcgctg cttctgcaag cagctggtta
     1201 cgcgcctgag tcagtttttc gtgaccaagg ccagcctggt caatcagctc aaagtcaaag
     1261 ccggttgcag tacccagttc cacgattgcg ggcaggttaa aggcgaaaac catcgcatct
     1321 ttgatttgcg agaaagcgcg tgttgcacgc atggtaatcg cttcaacttt gttttcttcg
     1381 cccggacgat cggcccagtc cttcaaggaa acgaacgcaa taccggtatt ctgaccacgt
     1441 cccgcaaagc cgaagccgtt aacggcgaac accgactcaa cgttgttctt ttctttggtc
     1501 agatagtaat gcgttacctc attgagcact ttctgcgtac gttcctgcgt tgcacctgct
     1561 ggcagctgaa ccatggtcat aaatacgccc tggtcctcat ctggcaagaa ggagcttggc
     1621 agacgcacga acagataggc catgccgacc acgatgatca gatacagcac caggtaacgc
     1681 cccgtactgc gcagaatacc gcctacgctg tcggtgtagt ggtgcgtgct cttctcgaac
     1741 atgcggttaa accagccgaa gaagcctttt ttaccttccc cgtgatcacc tttggcaatc
     1801 ggtttcagca tggtggcaca aagagccgga gtcaggatca acgctaccag taccgacagc
     1861 gccattgctg aaacaatggt aatagagaac tgacgataga tagcaccagt agaaccgcca
     1921 aagaaggcca tcggtacgaa taccgccgac agtaccatcg cgataccgac cagagcgccc
     1981 tgaatctgcc ccatcgactt acgggtagct tcttttggcg gcaaaccttc ttccgccata
     2041 acacgctcca cgttttctac cacaacgata gcgtcatcca ccaacaagcc gatagcgagc
     2101 accatcccga acattgttag cgtgtttatc gagaagccaa aggcggcaag gacggcaaag
     2161 gtcccgagca ataccaccgg tacggcaatg gtcggaatca acgtcgcgcg gaagttctgc
     2221 aggaacagat acataaccag gaacacgagg atgatcgctt cgaccagcgt tttaaccact
     2281 tcgtgaatag agattttcac gaacggcgtg gtgtcgtacg ggtaaacaat tttcagaccc
     2341 gacgggaaga acggttccat cttcgccagt tcagcacgga ttgccgcagc ggtatccagc
     2401 gcgtttgcac cggtcgccag cttgatcccc agaccggaag ccggttggcc gttaaactct
     2461 gcgatgatgt cgtagttctc accacccagc tcaatcttcg ccacatcacg cagcagtacg
     2521 cgggaaccat cttgattcac tttcagcagg attttgccga actcttcagt agaggtcaga
     2581 cgcgtctgag caataataga ggcgttaagc tgttggcctt tcaccggcgg cgtaccaccg
     2641 agctgacccg ccgcaacctg ggcgttctgc gctttgatgg cggtaatgac atcaaccggc
     2701 gttagctgga atttgttcag ctcattcggg ttcatccaga tacgcatcgc gtactgtgaa
     2761 ccgaacaact gaacatcacc cacgcccgac gtacggctga tggcatcttt catattcgcc
     2821 gctacgtagt cagagatatc ctcctgcgtc atggtgccat cggtgttgat aacgccgaca
     2881 accatcagga agctgctgga tgatttctca acgctcaccc cttgctgctg aacttcttgc
     2941 ggcagcaacg gcatcgccag ctgcagtttg ttctgcacct gaacctgcgc gatatccgca
     3001 tcagtaccag actcaaaggt cagggtgatc tgcacggtac ccgtggagtc actgttagag
     3061 gacatgtaca tcaggttatc gataccgttc atattctgtt cgataacctg tgtcaccgtg
     3121 tcctgcactg ttttcgcatc agcgccgggg taggaggcgg agatcgttac tgccggcggt
     3181 gcaatcgtag gatattgcgc caccggcagt ttgaggatcg ccagcccccc tgccaacatg
     3241 atgataatgg cgatcaccca cgcaaaaatc gggcgatcga taaagaaatt aggcatgtct
     3301 taacggctcc tgtttaagtt aagacttgga ctgttcaggc tgagcaccgc ttgcggcttg
     3361 ctggttatta tcagcggtaa cttcttgtgc ttttacctgg acaccaggac gcactttctg
     3421 cagcccactt attactacgc gatcgcctgc tttcagacct tctgtcacca gccacttatc
     3481 gccaatagcc tggcttgcaa cgatcggacg ggtttccact ttgtcatccg cgccaactac
     3541 cagtacggtg gcatcaccac gcggcgtacg ggttacgccc tgttgcggga ctaaaatagc
     3601 gtttggatta agcccttctt ccagacgtgc gcgcacgaac atacccggca gcagagtgtg
     3661 atccgggttc gggaagatag cgcgtagggt gatagaccca gtggtctgat caacggtaac
     3721 gtcagagaat tccagcgtac cgtcctgcgg gaacttaatg ccgtcactgg tgatcagcga
     3781 cactttggct ttgccgttct cttgtttcag cgtgccattc gccagttcct gtttcaggcg
     3841 caggaagtcg ttgctggact gggtcacatc aacgtagatc ggatcaagtt gctgcacggt
     3901 tgccagcgca gtcgcctgac cgttctgtac caatgcgcct tccgtcacgt tcgacttacc
     3961 aatgcgaccg ctaatcggag aggtgacttt ggtgtaagcc agattgatcc gcgcagtttc
     4021 aacggcagct ttcgccgcag ttaccgcagc attcgcctgt tgcgcatcag ccagagcctg
     4081 atcgtactct tgcttactga tgtactgagt accgagcagt ttctgataac gattcaccgt
     4141 caattgcgcg atattggctg cagcctgggc tttcgccaga tcacctttcg cactgtcgta
     4201 tgtcgcctga taggtcgcag gatcaatctg atagagagag acacctgctt cgatgtcgct
     4261 accttctttg aaattacgct tcaggataat cccgctaact tgaggacgaa cttctgcgat
     4321 ccggtaggca ctggtgcgac ccggaagctc ggttgtgatc tgcagaggtt cagttttgac
     4381 tgttactacg ccaacggcgg gcatctgctg gccaccttgt tgggcctgtt tgtcgtcaca
     4441 tcctgttagg gctaagctgc ctgagagcat cagaacgacc gccagaggcg taaaccctct
     4501 gtttttgttc atatgtaaac ctcgagtgtc cgatttcaaa ttggtcaatg gtcaaaagtt
     4561 aataaaccca ttgctgcgtt tatattatcg tcgtgctatg gtacatacat tcacaaatgt
     4621 atgtaaatct aacgcctgta aattcacgaa catatggcac gaaaaaccaa acaagaagcg
     4681 caagaaacgc gccaacacat cctcgatgtg gctctacgtc ttttctcaca gcagggggta
     4741 tcatccacct cgctgggcga gattgcaaaa gcagctggcg ttacgcgcgg tgcaatctac
     4801 tggcatttta aagacaagtc ggatttgttc agtgagatct gggaactgtc agaatccaat
     4861 attggtgaac tagagcttga gtatcaggca aaattccctg gcgatccact ctcagtatta
     4921 agagagatat taattcatgt tcttgaatcc acggtgacag aagaacgacg tcgattattg
     4981 atggagatta tattccacaa atgcgaattt gtcggagaaa tggctgttgt gcaacaggca
     5041 caacgtaatc tctgtctgga aagttatgac cgtatagaac aaacgttaaa acattgtatt
     5101 gaagcgaaaa tgttgcctgc ggatttaatg acgcgtcggg cagcaattat tatgcgcggc
     5161 tatatttccg gcctgatgga aaactggctc tttgccccgc aatcttttga tcttaaaaaa
     5221 gaagcccgcg attacgtcgc catcttactg gagatgtatc tcctgtgccc cacgcttcgt
     5281 aatcctgcca ctaacgaata accctgaatc tgactccagg atttttcctg gacattttcg
     5341 tcgttgctat tctggttcac tgcgtcgtga tattcttgcg gtttgacttt ttcaggtcgt
     5401 tcttcaggtt cagaaacctt cattcatcat gactatgttc cagtattaca aacgatcacg
     5461 gcattttgtt ttttcagcat ttattgcttt tgtttttgtc ttgttatgcc agaacacggc
     5521 gtttgcgcgg gcgtcatcga atggtgatct gccgacaaaa gcggacctgc aggcgcaact
     5581 tgactcacta aataaacaaa aagatctttc tgctcaggac aaactggtgc agcaggatct
     5641 gacagataca ttagccaccc tcgataaaat cgatcgcata aaagaagaga cagttcagct
     5701 acggcaaaaa gtcgctgaag cgccggaaaa aatgcgccag gcgaccgcgg cgttaacagc
     5761 acttagcgat gtcgataacg acgaagaaac gcgcaaaatt ctgagcacgc tttcgttgcg
     5821 ccagctggaa actcgcgttg cccaggcgct ggacgatttg caaaacgcac aaaacgatct
     5881 ggcgtcttat aacagccagc tggtttcgtt acagacgcag cccgaacgcg tgcaaaatgc
     5941 gatgtataac gcttcgcagc agctgcaaca aattcgcagt cgtctggatg ggactgatgt
     6001 cggcgagaca gccttacgtc ccagccagaa agtgttaatg caggcccagc aggcgttgct
     6061 gaatgcggag attgaccagc agcgtaaaag cctggaaggg aacaccgtct tgcaggatac
     6121 cttgcaaaag caacgtgatt acgtgacggc gaacagcgct cgtctggagc accagttaca
     6181 actgttgcaa gaagcggtaa acagcaagcg cctgacttta accgaaaaaa cggcgcagga
     6241 agccgtctcc ccggatgaag ccgcgcgtat tcaggctaat ccgctggtga agcaggaact
     6301 ggaaattaac cagcagttaa gtcagcgtct gattaccgcg actgaaaacg gtaatcagtt
     6361 gatgcagcaa aacattaaag tcaaaaactg gctggagcgg gcgctgcaat cggaacgcaa
     6421 tattaaagag cagattgccg tcctgaaggg tagcctgctg ttgtctcgta tcctttacca
     6481 gcaacaacaa acgctgccct cggcggatga actggaaaac atgaccaacc gcatcgcgga
     6541 tttgcgtctc gaacagtttg aagttaacca gcagcgtgat gcactcttcc agagcgatgc
     6601 gttcgtcaac aaactggaag aaggtcacac caacgaagtc aacagcgaag ttcacgatgc
     6661 gttattgcaa gtggttgata tgcgtcgcga attgctggat caactcaaca aacagttggg
     6721 taaccagctg atgatggcca ttaacctgca aatcaaccag cagcagttaa tgagcgtatc
     6781 gaaaaacctg aaatccatcc tgactcagca aatcttttgg gtgaacagta accgtccaat
     6841 ggactgggac tggatcaaag cgttcccgca aagcctgaaa gatgaattta agtcgatgaa
     6901 aatcacggtg aactgggaaa aagcctggcc tgccgttttt atcgctttcc tcgccggttt
     6961 gccgctgctg ttgattgccg ggctgatcca ctggcgtctg ggctggctga aagcgtatca
     7021 acagaaactg gcttccgctg tgggttccct gcgtaacgat agccagctca acacaccaaa
     7081 agcgatcctt atcgacctga tccgcgcgct gccggtgtgc ctgattattc tcgcggttgg
     7141 cctgattctg ttgaccatgc agctcaacat cagcgaactg ctatggtcgt tcagtaaaaa
     7201 actggcgatt ttctggctgg tgtttggcct gtgctggaag gtactggaga aaaatggcgt
     7261 tgccgtgcgt cacttcggca tgccggagca gcagaccagc cactggcgtc ggcaaattgt
     7321 ccgcatcagt ctcgcgttgc tgcctatcca tttctggtct gtggtggcag aactttcccc
     7381 gctgcatctg atggatgatg tgctggggca ggcgatgatt ttcttcaacc tgctgctgat
     7441 tgccttcctg gtatggccaa tgtgccgcga aagctggcgt gataaagagt cgcacaccat
     7501 gcgactggtc accattaccg tgctgtcgat tatcccgatt gcattgatgg tgctgactgc
     7561 cacgggttat ttctacacca cgctgcgcct ctccgggcgt tggattgaaa ccgtttatct
     7621 ggtgatcatc tggaacttgc tctaccagac ggtattgcgc ggcttaagcg tagcggcgcg
     7681 gcgtatcgcc tggcgtcgtg cgctggcgcg tcggcagaat ctggtgaaag agggcgcaga
     7741 aggtgccgaa ccgccggaag aacccaccat tgcactggaa caggttaacc agcagacgct
     7801 gcgtattacc atgttgctga tgtttgcgct gttcggtgtc atgttctggg caatttggtc
     7861 cgatttgatc accgtgttca gctatctcga cagcatcacg ctctggcatt acaacggcac
     7921 tgaagctggc gctgcggtgg tgaaaaacgt caccatgggc agtctgttgt ttgcgattat
     7981 cgcctcaatg gtggcctggg cgttgattcg caacctgcct ggtttactgg aagtgctggt
     8041 gctctcgcga ctgaatatgc gtcagggcgc gtcgtatgcc attactacca tccttaacta
     8101 catcattatt gccgttggtg cgatgacggt gttcggatcg ctgggcgtct cttgggataa
     8161 actccagtgg ctggccgcag cattatccgt aggtcttggt tttggtttac aagaaatttt
     8221 cggtaacttc gtctccggtt tgatcattct attcgaacgt ccggtgcgta ttggcgatac
     8281 ggtaaccatt ggtagcttct cggggacggt aagtaagatc cgtattcgtg cgacaacgat
     8341 taccgatttc gatcgcaaag aagtgatcat cccgaacaaa gcgtttgtta ccgagcgtct
     8401 gatcaactgg tcgttgactg acactactac gcgtctggtg atccgtctcg gcgtggccta
     8461 tggctccgat ctggaaaaag tgcgtaaagt gttactgaag gcggcgactg agcacccaag
     8521 ggtgatgcac gaaccaatgc cggaagtctt ctttacggca tttggtgcca gcacgttgga
     8581 tcatgagctg cgtctgtatg tgcgtgaact gcgtgaccgt agtcgtactg tcgatgagct
     8641 gaaccgtact atcgatcagc tgtgccgtga aaacgacatc aacattgcct ttaaccagct
     8701 tgaagtgcat ctgcacaacg agaagggcga tgaggtgacg gaagtaaaac gcgactacaa
     8761 aggcgatgac ccgacgccag cggtagggta aaaacgaaag ggggcaacat ttagttgccc
     8821 cgagattgct aacaaagtgc gcgttgttca tgccggatgc ggcgtgaccg ccttatccgg
     8881 cctacgaaac cgcaagaatt caatatattg caggagcggt gtaggcctga taagcgtagc
     8941 gcatcaggca gttttgcgtt tgcccgcaac cttaggggac atttagcgac cccatttatt
     9001 tctcactttt ccgcctcatc atcgcgcgtt aatttctttt cataatcacg ctttacaata
     9061 tccagcgcgc gcagcacggt actggcaggg atctgatttt cctccagcag cacaatcaaa
     9121 tcgacagcca gtttgacatc gtcaggggca ttttccagtg acatattctc tccattgcta
     9181 gcgggttaaa cgcgctaacc tgttttcgat tttttccagc gcatggcggc agcgtgccag
     9241 gcgcgcttca taggcttcca cttcacgatg cagcgtttgc tgttccacga gatcggtcac
     9301 ccgcgccaga cgggctctgc gttcggcaac catctcacgt agccgccgct caaaatcctg
     9361 atgctgaata cgtttacgct gccagcgggc aattttcggt ggtgcactgt cccactcgcg
     9421 caatgaccag gcggaggctt cacgcgcgat ggcttccagt tgtgccgcca gatgttccgc
     9481 cagccaggcc acttgcggca gctgttgctg ctcaactgca tgacgaagcg cagccagatt
     9541 atcgcccgcc tcatcgagac aagcctgcag tgttgtcgca cgggtttgaa aaaggtgtct
     9601 gtcaaaacgg gcacttagcg tggcaaattg cgctaccggg gcacaacgct gacgcagcgt
     9661 agcgagctgt ccttccagtt tttccagcag cagggcggtt ttcacgataa gacctcaatg
     9721 aaaatgataa ttgttatgct aaagtagcca ctctgtaagc tgacaacact caatatgcaa
     9781 cgaatcattt taatcatcat tggctggctg gcggtagtgc tgggtacgct gggcgtggta
     9841 ttaccggtat taccgacgac gccgtttatc ctgctggcgg cctggtgctt tgcccgttct
     9901 tccccgcgct ttcacgcctg gttgctgtac cgctcatggt ttggcagcta tctacgtttc
     9961 tggcagaaac atcatgcgat gccgcgcggc gtcaaaccgc gggcgatttt gcttattttg
    10021 ctcacgtttg ccatttctct gtggttcgtc cagatgccac gggtgcgcat catgttgctg
    10081 gtaattctcg cctgtttgct tttctatatg tggcgaattc cggtgattga tgaaaagcaa
    10141 gaaaagcact gaagcacaac aatcgcagtt gcaattattg cgtacagcca gtacattctg
    10201 gcgttttcga gcacaggcgc aggcggtcaa aggttaaaca actgttactt ttgatacgtt
    10261 taaaacgcgc cgtgagtacc accgtaacaa gcaggcatac acttatgacc gcgactgcac
    10321 agcagcttga gtatctcaaa aatagcatca aaagcattca ggactaccca aaacccggca
    10381 ttcttttccg cgatgtcacc agcttactgg aagacccgaa agcttacgct ctcagcatcg
    10441 acttgctggt tgagcgttac aaaaatgcgg gcattaccaa agttgtcggc accgaagcgc
    10501 gtggcttctt gtttggcgct ccggtagctc tgggtctggg cgttggcttt gtaccggtcc
    10561 gtaaaccggg caaactgccg cgtgaaacca tcagtgaaac ttacgacctg gaatacggca
    10621 ccgatcagct ggagatccac gttgatgcca tcaaaccggg cgacaaagtt ctggtggtgg
    10681 acgatctgct ggcaaccggc ggcactatcg aagcgaccgt taaactgatc cgtcgtctgg
    10741 gtggtgaagt ggctgacgct gcgttcatta tcaacctgtt cgatctcggc ggcgaacagc
    10801 gtctcgaaaa acagggcatt accagctaca gccttgtccc gttcccgggc cattaattat
    10861 cgccagtctt gtgctgccca cgctacggac agcacaagat gtgcattcag cctcgccgtt
    10921 ctgacggggc tgtgttagca ttaccccttc gtgaatccac cttccagcgt ttcagagcct
    10981 gccaatgagt tatcaggtct tagcccgaaa atggcgccca caaacctttg ctgacgtcgt
    11041 cggccaggaa catgtgctga ccgcactggc gaacggcttg tcgttagggc gtattcatca
    11101 tgcttatctt ttttccggta cccgtggcgt cggaaaaacc tctatcgccc gactgctggc
    11161 gaaggggcta aactgcgaaa ccggcattac cgcgacgccg tgcggcgtgt gcgataactg
    11221 tcgtgaaatc gagcaggggc gctttgtcga tctgattgaa atcgacgccg cctcgcgcac
    11281 caaagttgaa gatacccgcg acctgctgga taacgtccag tacgctccgg cgcgtggtcg
    11341 tttcaaagtt tatctgatcg acgaagtgca tatgctgtcg cgccacagct ttaacgcact
    11401 gttaaaaacc cttgaagagc cgccggagca cgttaagttt ctgctggcga cgaccgatcc
    11461 acagaaattg ccggtgacga ttttgtcgcg ctgtttgcaa tttcatctca aggcgctgga
    11521 tgtcgagcaa attcgccatc agcttgagca catcctcaac gaagaacata tcgcccacga
    11581 gccgcgggcg ctgcaattgc tggcgcgcgc cgctgaaggc agcctgcgag atgccttaag
    11641 tctgaccgac caggcgattg ccagcggtga cggccaggtt tcaacccagg cggtcagtgc
    11701 gatgctgggt acgcttgacg acgatcaggc gctgtcgctg gttgaagcga tggtcgaggc
    11761 caacggcgag cgtgtaatgg cgctaattaa tgaagccgct gcccgtggta tcgagtggga
    11821 agcgttgctg gtggaaatgc ttggcctgtt gcatcgtatt gcgatggtac aactttcgcc
    11881 tgctgcactt ggcaacgata tggccgccat cgagctgcgg atgcgtgaac tggcgcgcac
    11941 cataccgccg acggatatcc agctttacta tcagacgttg ttgattggtc gcaaagaatt
    12001 accgtatgcg ccggaccgcc gcatgggcgt tgagatgacg ctgctgcgcg cgctggcgtt
    12061 ccatccgcgt atgccgctgc ctgagcctga agtgccacgc cagtcctttg cacccgtcgc
    12121 gccaacggca gtaatgacgc caacccaggt gccgccgcaa ccgcaatcag cgccgcagca
    12181 ggctccgact gtaccgctcc cggaaaccac cagccaggtg ctggcggcgc gccagcagtt
    12241 gcagcgcgtg cagggagcaa ccaaagcaaa aaagagtgaa ccggcagccg ctacccgcgc
    12301 gcggccggtg aataacgctg cgctggaaag actggcttcg gtcacagatc gcgttcaggc
    12361 gcgtccggtg ccatcggcgc tggaaaaagc gccagctaaa aaagaagcgt atcgctggaa
    12421 ggcgaccact ccagtgatgc agcaaaaaga agtggtcgcc acgccgaagg cgctgaaaaa
    12481 agcgctggaa catgaaaaaa cgccggaact ggcggcgaag ctggcggcag aagccattga
    12541 gcgcgacccg tgggcggctc aggtgagtca actttcgcta ccaaaactgg tcgaacaggt
    12601 ggccttaaat gcctggaaag aggagagtga taacgcagta tgtctgcatt tgcgctcctc
    12661 tcagcggcat ttgaacaacc gcggtgcaca gcaaaaactg gctgaagcgt tgagcatgtt
    12721 aaaaggttca acggttgaac tgactatcgt tgaagatgat aatcccgcgg tgcgtacgcc
    12781 gctggagtgg cgtcaggcga tatacgaaga aaaacttgcg caggcgcgcg agtccattat
    12841 tgcggataat aatattcaga ccctgcgtcg attcttcgat gcggagctgg atgaagaaag
    12901 tatccgcccc atttgatcgt aagcacagct tacgttcgtc atccttaacg tgattgagag
    12961 agaaacctat gtttggtaaa ggcggtctgg gtaacctgat gaagcaagcc cagcagatgc
    13021 aagaaaaaat gcagaaaatg caggaagaga tcgcgcagct ggaagttacc ggcgaatctg
    13081 gcgcaggtct ggtaaaagtg accatcaacg gtgcacataa ctgccgtcgc gtagagatcg
    13141 acccgagcct gctggaagac gacaaagaga tgctggaaga cctggtggct gcagcattca
    13201 acgacgcagc acgtcgtatc gaagaaacgc agaaagaaaa aatggcttct gtatcctccg
    13261 gaatgcagct gccgcctggc tttaagatgc cgttctgatg caaaccagcc cgctgttaac
    13321 acagcttatg gaagcactgc gctgtctgcc gggcgttggc ccgaagtcgg cgcagcgtat
    13381 ggcgttcacg ctgcttcagc gcgatcgtag cggcgggatg cgtctggcgc aggcgctcac
    13441 ccgggcgatg tcggaaatcg gccactgcgc cgattgccgc actttcaccg aacaggaagt
    13501 ctgtaacatc tgttcgaatc cgcgtcgtca ggaaaacggt caaatctgtg tggtggagag
    13561 tccggcggac atctacgcca ttgagcagac ggggcagttt tctggtcgtt attttgtgtt
    13621 gatggggcat ctgtcaccgc tggacggcat cggtccggat gatatcgggc ttgatcgtct
    13681 ggaacagcgt ctggcagagg aaaaaatcac tgaagtgatc ctcgccacca atcccacggt
    13741 tgaaggtgaa gctaccgcta actacattgc cgagctttgc gcgcaatatg acgtggaagc
    13801 cagccgaatc gctcatggcg taccggttgg cggcgaactg gaaatggtcg atggcaccac
    13861 tctgtcacac tcccttgccg ggcgtcataa gattcgtttt taagcaaacg agagcaggat
    13921 cacctgctct cgcttgaaat tattctccct tgtccccatc tctcccacat cctgttttta
    13981 accttaaaat ggcattattg aggtagacct acatgaaagg acaagaaact cgtggttttc
    14041 agtcagaagt gaaacagctt ctgcacctga tgatccattc tctctattcc aataaagaaa
    14101 tcttcctgcg tgagcttatc tctaacgcct ccgatgcggc ggacaagctg cgtttccgcg
    14161 cgctctctaa cccggacctg tacgaaggtg atggcgaact gcgcgttcgt gtctctttcg
    14221 ataaagacaa gcgcacgctg accatctcag ataacggcgt ggggatgacc cgcgatgaag
    14281 tgatcgacca tctggggact atcgctaaat ccggcaccaa atcattcctc gaatccctgg
    14341 gttctgacca ggcgaaagac agccagctga tcggtcagtt cggtgttggt ttctactctg
    14401 cgtttatcgt ggccgacaaa gtgaccgtgc gtactcgcgc ggcaggcgaa aaaccagaaa
    14461 atggcgtgtt ctgggaatcg gctggtgaag gtgaatacac cgttgccgac atcaccaaag
    14521 aagatcgtgg tactgaaatc accctgcatc tgcgtgaagg cgaagacgag ttcctcgatg
    14581 actggcgcgt gcgttccatc atcagcaaat actccgacca tatcgcgctg ccggtagaga
    14641 tcgaaaaacg cgaagagaaa gacggcgaaa ccgttatctc ctgggagaaa atcaacaaag
    14701 cgcaggcgct gtggactcgt aacaagtcgg aaatcaccga tgaagagtac aaagagttct
    14761 acaaacacat cgcccacgac tttaacgatc cgctgacctg gagccacaac cgcgttgaag
    14821 gtaagcagga gtacaccagc ctgctgtaca ttccgtccca ggctccgtgg gatatgtgga
    14881 accgcgatca taaacacggc ctgaaactgt acgtccagcg agtgttcatc atggacgacg
    14941 cagaacagtt catgccgaac tatctgcgct tcgtgcgtgg tctgattgac tccagcgatc
    15001 tgccgctgaa cgtttcccgt gaaatcctcc aggacagcac ggtaacgcgt aacctgcgca
    15061 atgcgctgac caagcgtgtg ctgcaaatgc tggaaaaact ggcgaaagac gacgcggaaa
    15121 aataccagac cttctggcaa cagtttggcc tggtactgaa agaaggtccg gcggaagatt
    15181 tcgctaacca ggaagcgatc gccaaactgt tgcgttttgc ttctacccat accgattctt
    15241 ctgcgcagac cgtgtctctg gaagactacg tttcccgcat gaaagaaggg caggagaaaa
    15301 tctactacat cactgcagac agctatgcgg cagcgaagag cagtccgcac ctggaactgc
    15361 tgcgtaagaa aggcatcgaa gttctgctgc tttccgaccg cattgatgag tggatgatga
    15421 actatctgac tgagtttgac ggtaaaccgt tccagtctgt gtctaaagtt gacgagtcgc
    15481 tggaaaaact ggctgacgaa gttgatgaga gcgcgaaaga agcggagaaa gcactgactc
    15541 cgttcatcga ccgtgtgaaa gccctgctcg gcgagcgcgt gaaagatgtc cgtctgactc
    15601 accgtctgac cgatacgcca gcgatcgttt cgaccgacgc ggatgaaatg agcacccaga
    15661 tggcgaaact gttcgctgcg gcgggccaga aagtgccaga agtgaaatac atcttcgaac
    15721 tgaacccgga tcacgtactg gtgaaacgtg cggcagatac tgaagatgaa gcgaagttca
    15781 gcgagtgggt agaactgctg ctggatcagg cgctgctggc agaacgcggc acgctggaag
    15841 atccgaatct gtttattcgt cgtatgaacc agctgctggt ttcctgatgt catgccggat
    15901 gaccttcgtg tcatccggca tttttctttt catcatctgc actttccgca aattatctcg
    15961 ccattaaccg tttcagcctc aggtgccttt cttgaggcaa tcgcctgttg gtggtatcgt
    16021 ttatcgcttt ttcaaaaaat tcgacacatt ttaaggggat tttcgcaatg cgtatcattc
    16081 tgcttggcgc tccgggcgcg gggaaaggga ctcaggctca gttcatcatg gagaaatatg
    16141 gtattccgca aatctccact ggcgatatgc tgcgtgctgc ggtcaaatct ggctccgagc
    16201 tgggtaaaca agcaaaagac attatggatg ttggcaaact ggtcaccgac gaactggtga
    16261 tcgcgctggt taaagagcgc attgctcagg aagactgccg taatggtttc ctgttggacg
    16321 gcttcccgcg taccattccg caggcagacg cgatgaaaga agcgggcatc aatgttgatt
    16381 acgttctgga attcgacgta ccggacgaac tgatcgttga ccgtatcgta ggccgccgcg
    16441 ttcatgcgcc gtctggtcgt gtttatcacg ttaaattcaa tccgccgaaa gtagaaggca
    16501 aagacgacgt taccggtgaa gaactgacta cccgtaaaga cgatcaggaa gaaaccgtgc
    16561 gtaaacgtct ggttgaatac catcagatga ctgcaccgct gatcggctac tactccaaag
    16621 aagcggaagc gggtaacacc aaatacgcga aagttgacgg caccaagccg gttgctgaag
    16681 ttcgcgctga tctggaaaaa atcctcggct aattcgaaag cgcgcacgga cagtcccctc
    16741 gccccctcgg ggagagggtt agggtgaggg gaacaggccc gcacaagcaa acttatcagc
    16801 aatctcaggc cggatattca ttcggccttt tacaaaaacc tcaatctgcg ctattccttt
    16861 ttctgatttg acctctcaca gcaattagtt cttcttcctc acttttccgc tacaattatc
    16921 aacaagttga atcgataaga ggcggtaatg cgtcagacta aaaccggtat cctgctggca
    16981 aacctgggta cgcccgatgc ccccacacct gaagcggtaa aacgctatct gaaacaattt
    17041 ttaagcgaca gacgcgtggt tgatacctca cggttgttat ggtggccgtt gctgcgcggc
    17101 gtgattttgc cgctgcgctc gccgcgtgtg gcgaagctgt acgcctcggt ctggatggaa
    17161 gacggctcgc cgctgatggt ttacagccgc cagcaacagc aggcgttggc acaacgttta
    17221 ccggatacgc cagtggcgct gggcatgagc tacggctcgc catcactgga aagcgccgtc
    17281 gatgaactcc tggcagagca tgtagatcat attgtggtgc tgccgcttta tccgcaattc
    17341 tcctgttcta cggtcggtgc ggtatgggat gaactggcac gcattctggc gcgcaaacgt
    17401 agcattccgg ggatatcgtt tattcgtgat tacgccgata accacgatta cattaatgca
    17461 ctggcgaaca gcgtacgcgc ttcttttgcc aaacatggcg aaccggatct gctgctgctc
    17521 tcttatcatg gcattcccca gcgttatgca gatgaaggcg atgattaccc gcaacgttgc
    17581 cgcacaacga ctcgcgaact ggcttccgca ctggggatgg caccggaaaa agtgatgatg
    17641 acctttcagt cgcgctttgg ccgggaaccc tggctgatgc cttataccga cgaaacgctg
    17701 aaaatgctcg gagaaaaagg cgtaggtcat atacaggtga tgtgcccggg ctttgctgcg
    17761 gattgtctgg agacgctgga agagattgcc gagcaaaacc gtgaggtctt cctcggtgcc
    17821 ggcgggaaaa aatatgaata tattccagcg cttaatgcca cgccggaaca tatcgaaatg
    17881 atggctaatc ttgttgccgc gtatcgctaa agctgagcgg taaagaactg agcgccgtcg
    17941 cgaagagcct cgtcggcggt tttcatcatt cgtgaataat gcaaaaaggc atgcagcgta
    18001 cctgggtaga gtttgaactc acagggctgc tgatgcgccg ctaacgtctg gtaaagcaga
    18061 cggctgtcat ccagcagcgg atcgaactcc gccccggcaa taaaacaggg cggaacttcg
    18121 cgagtgagat cattattaaa cagacagtaa tacggtgact cgcggtccgc gtcgttgctt
    18181 aaatacgcct cttcatacat ctgcaaatcc tgttgcgtta agccatccca gacaccgccc
    18241 aacagacgac gagtcacgga atcccgtaat ccgtaaagcc cataccacag caaaacaccc
    18301 gcaattttac cgcaatcgat ctgtttatca cgcaaccaca acgcactggc gagcgccagc
    18361 atggcacctg cggaatcacc ggcaaagcca atgcgggaca tattgatttg ataatcctcc
    18421 gcctgctggt ggaaataaca acaagcagcc acaatttcct ctatcgcttg cggaaaacgc
    18481 gcttcaggtg aaagggggta atcaatacca atcaccgtac attggctgta gcttgccagc
    18541 aggcgcatga tgcgatcgtg ggtatcgaga ttgccgagaa taaaaccgcc tccatgcaaa
    18601 taaaatagcg tcgccgggct atctggctgc ggacaaaaga gacgtgtttc cacctgccca
    18661 tattttgttg gaaccatgta agctctggtt gccatttctg gagcgcccgc attccagaat
    18721 cggcgctcaa gcgtgtaata ctgtcgttgc tcagcaatcg ttcccgttgc gggccaggac
    18781 ggtaaatccg actgaagagt attcacaacg gtcttcattt cagcagaaat aaggtccaga
    18841 acaggtagtt tgttttccgg cttcatacaa aaactccttt caaattacgt cattgtaagg
    18901 aaccactgcc atgaaaatgc gatcccgcct gctgatattg aaactggctg cgtctcgcgc
    18961 gctcccgtca gattgtgtta acattcgccg ctcagttaac cacccgtaaa aacaaccatg
    19021 aaatttcccg gtaaacgtaa atccaaacat tacttccccg taaatgcacg cgatccgctg
    19081 cttcagcaat tccagccaga aaacgaaacc agcgccgcct gggtcgtagg tattgaccag
    19141 acgctggtcg atattgaagc gaaagtggat gatgaattca ttgagcgtta tggattaagc
    19201 gccgggcatt cactggtgat tgaggatgac gtagccgaag cgctttatca ggagctaaaa
    19261 cagaaaaacc tgattaccca tcagtttgcg ggtggcacca ttggtaacac catgcacaac
    19321 tactcggtgc tcgcggacga ccgttcggtg ctgctgggcg tcatgtgcag caatattgaa
    19381 attggcagtt atgcctatcg ttacctgtgt aacacttcca gccgtaccga tcttaactat
    19441 ctacaaggcg tggatggccc gattggtcgt tgctttacgc tgattggcga gtccggggaa
    19501 cgtacctttg ctatcagtcc aggccacatg aaccagctgc gggctgaaag cattccggaa
    19561 gatgtgattg ccggagcctc ggcactggtt ctcacctcat atctggtgcg ttgcaagccg
    19621 ggtgaaccca tgccggaagc aaccatgaaa gccattgagt acgcgaagaa atataacgta
    19681 ccggtggtgc tgacgctggg caccaagttt gtcattgccg agaatccgca gtggtggcag
    19741 caattcctca aagatcacgt ctctatcctt gcgatgaacg aagatgaagc cgaagcgttg
    19801 accggagaaa gcgatccgtt gttggcatct gacaaggcgc tggactgggt agatctggtg
    19861 ctgtgcaccg ccgggccaat cggcttgtat atggcgggct ttaccgaaga cgaagcgaaa
    19921 cgtaaaaccc agcatccgct gctgccgggc gctatagcgg aattcaacca gtatgagttt
    19981 agccgcgcca tgcgccacaa ggattgccag aatccgctgc gtgtatattc gcacattgcg
    20041 ccgtacatgg gcgggccgga aaaaatcatg aacactaatg gagcggggga tggcgcattg
    20101 gcagcgttgc tgcatgacat taccgccaac agctaccatc gtagcaacgt accaaactcc
    20161 agcaaacata aattcacctg gttaacttat tcatcgttag cgcaggtgtg taaatatgct
    20221 aaccgtgtga gctatcaggt actgaaccag cattcacctc gtttaacgcg cggcttgccg
    20281 gagcgtgaag acagcctgga agagtcttac tgggatcgtt aagttatcgt cggttcgtag
    20341 gccagataag gcgttcacgc cgcatctggc atttggctct cgatgcccga tgcgacgctg
    20401 gcgcgtctta tcatgcctac atatttttca tattttacat ccggcaacca ccgtttaccc
    20461 cgtcaccact tcacccgccg gtggcgtttc cagcagttcc agcatagtac gggcaatttc
    20521 acgttcaccc atcactacct gattcgcgcc acgttcggtg atatacgtca cttcatcgtc
    20581 ataatgggcg cgggcgataa tctcaatatc cgggtttttc gcgcgggcag atgccacaat
    20641 ctcgcctgct tcataaccgt tgggaatcgt caggatcagc cattttgcac attccagatg
    20701 cgccagttgc ataatttctt cgttcgccgc attgcccaat actgcgcgga ccccgcgctc
    20761 tcgcagctca tcaacacggg ttcgtgacgt ctcaatcacc accagcggaa tatcagaggc
    20821 gagcaatttc tcccccagca ggctgcctac acgaccgtaa cccaccagaa gcgcatggtt
    20881 gcaaatatcc actgggatct gcttctcttc ttcgattgcc tcttccagcg tctgctcttc
    20941 cagcgtttcg gtcttcgcca gatatttctc cagtagtgcg aacagtaccg ggttgagcat
    21001 aatcgacagg atcgcccctg ccagtaccag gttttgtccg gcctgcggca gtaaattcaa
    21061 tgccattccc agtcccgcca ggataaacgc gaactcacca atctgcgcca ggctggcggc
    21121 gatggttaat gccgtacgtt gggagtgacc aaacagtcgc accaggaaaa atgcggctaa
    21181 cgacttacca aacagaataa tcgccagcgt cgccagcact gccagcggtt gctgaatcag
    21241 aattaacgga tcaaacaaca tcccgacgga gacaaaaaac agcaccgcaa acgcgtcgcg
    21301 caatggcagc gtatcgtggg cggcacggtg actcagttca gactcgttca gtaccatccc
    21361 ggcaaagaac gcaccgagtg caaaggagac atcaaacagc tctaccgcac caaaggcaat
    21421 ccctaacgcc agcgccagca ccgacagggt aaacagctcg cgagaaccgg ttgccgcgct
    21481 gcgtgccata atccacggca ccagacggcg acctaccagc atcataatgg cgataaatgc
    21541 gatcactttg ccgatggtga tccccatatc gactgcaaga gtggcaaagc ccacatcgcc
    21601 ctgttccatc attcctgcca ctgcgggcag caacaccagc gtcagaacca ttaccaggtc
    21661 ttccacaatc aaccaaccga tggcgatttg cccacgctga ctgtcaatta attgccgttc
    21721 ttcaagtgcg cgcagtaaca ccacggtact ggcggtggaa agacataaac cgaacacgat
    21781 accggtcatt aacgaccagc ccagcacggc agagagcgcc atacccagca gcgtcgccac
    21841 ggctatctgg gcgatcgcac cgggaatggc gatggccttt accgccatca aatccttcag
    21901 cgaaaaatgc aaaccgacgc cgaacatcag caaaataacg cccagttcag ccagttccgg
    21961 cgcaagcttg gtatcggcaa caaagcccgg agtgaatggt cctgccagca cacccgctaa
    22021 cagatatccc accagaggag aaatacgtag tttattggcc agcatgccga ggataaaggc
    22081 gagcacaagg ccgccaacaa tggtggtgat aagcggggtg gcgtgatgca ttccgtctcc
    22141 ttttcctggt ggttattgtc catttttggc cggggaaacc aaaattacag gtaatagttt
    22201 atgacaattt cattgatgat gttcatgaat aattattgaa ttttgcagaa aaatggaatt
    22261 agctgcaaaa aaagcacgga tcggaaaaca gaaggcggtt taacagagga aaggcttatg
    22321 gcgactgtaa aacgatgcag ccaaagtttg cctttggctg caatgaaatc agtctttatg
    22381 ccggttatca ggcaggaata tggtcaacat ccccaatagt ggcaggaaag cacagatttt
    22441 atagactaac tcgatactgg tgtgatcggc gataagcccc agaactgccg ctcccagacc
    22501 tcccatacca aaagcaaaac cgaaaaagag tccagaaacc ataccgatac gtcccggaag
    22561 cagctcctga gcgtaaacca gaatggcaga gaatgccgaa gcgaggataa atccaataat
    22621 caccgttaaa acccccgtcc agtgcaggct ggcgtagggt aaaatcagcg taaacggcgc
    22681 aacgccgagg atagagcccc aaatcacata tttccgccca attttatccc ctacaggccc
    22741 gccgatcacc gtacctgccg caacggcaaa caggaaggca aacagatgaa gctgagcatt
    22801 ctggatagat aatccgaatt tttgcatcag ataaaaggtg taatagctgc tgatgctcgc
    22861 catatagaaa tatttcgaga aaatgaggat taacagaatg ctgaccgcca gtaccacttt
    22921 gttgcgcggc agtggattga taatcgtcgc tttgggtttt cctttattca ttcggtgctg
    22981 tgccgagtac caacggctga tttgcgccaa caccacgatc gccagcagtg ccgcaagcac
    23041 aaaccaggca acgttgcctt tgccataagg cgcgataatc accgccgcca gcaagggtcc
    23101 cagggaactg ccaaagttgc cgccgacctg aaagatagat tgcgccaggc catgccgccc
    23161 gccggaagcc atacgggcta cgcgagaaga ttccggatga aagaccgatg aaccggtacc
    23221 gaccagcgcc gccgccagca gaactgcgcc aaaactgccc gccagcgcaa gcagcaccag
    23281 accacttaag gtaaagcaca tgccaattgg caacgaccac ggcatcggat atttatcggt
    23341 ccagtagccg accactggtt gcagtagcga agaggcgagc tggaaggtga gggttatcat
    23401 gccaatctgc ataaatgtca gagaaaattc tgactgaagc agcggataaa tcgccagaat
    23461 cagcgattgg atcatgtcgt tcagcagatg tgagaggctg atagcaccta aaataccaaa
    23521 cgatgttcgg gccttggtcg ttgacgcagc cgcgcccgcc acaggctggg gttgttcact
    23581 cattgccata ggaaagtcac tttttcaggg ttgcgatgta aagaatgatc ttatttgtga
    23641 ttattacccg actaacatac ctgtatgcgt cgcctgaagg aagtctcaac gccgaataca
    23701 gaatttctaa tctggatgca gatttatctt caccggacgc agacttgtct atgatgtcgc
    23761 gtcatactat ttttcaacac gttgaaatca ggtcagggag agaagtatga aattattgca
    23821 gcggggcgtg gcgttagcgc tgttaaccac atttacactg gcgagtgaaa ctgctctggc
    23881 gtatgagcag gataaaacct acaaaattac agttctgcat accaatgatc atcatgggca
    23941 tttttggcgc aatgaatatg gcgaatatgg tctggcggcg caaaaaacgc tggtggatgg
    24001 tatccgcaaa gaggttgcgg ctgaaggcgg tagcgtgctg ctactttccg gtggcgacat
    24061 taacactggc gtgcccgagt ctgacttaca ggatgccgaa cctgattttc gcggtatgaa
    24121 tctggtgggc tatgacgcga tggcgatcgg taatcatgaa tttgataatc cgctcaccgt
    24181 attacgccag caggaaaagt gggccaagtt cccgttgctt tccgcgaata tctaccagaa
    24241 aagtactggc gagcgcctgt ttaaaccgtg ggcgctgttt aagcgtcagg atctgaaaat
    24301 tgccgttatt gggctgacaa ccgatgacac agcaaaaatt ggtaacccgg aatacttcac
    24361 tgatatcgaa tttcgtaagc ccgccgatga agcgaagctg gtgattcagg agctgcaaca
    24421 gacagaaaag ccagacatta ttatcgcggc gacccatatg gggcattacg ataatggtga
    24481 gcacggctct aacgcaccgg gcgatgtgga gatggcacgc gcgctgcctg ccggatcgct
    24541 ggcgatgatc gtcggtggtc actcgcaaga tccggtctgc atggcggcag aaaacaaaaa
    24601 acaggtcgat tacgtgccgg gtacgccatg caaaccagat caacaaaacg gcatctggat
    24661 tgtgcaggcg catgagtggg gcaaatacgt gggacgggct gattttgagt ttcgtaatgg
    24721 cgaaatgaaa atggttaact accatctgat tccggtgaac ctgaagaaga aagtgacctg
    24781 ggaagacggg aaaagcgagc gcgtgcttta cactcctgaa atcgctgaaa accagcaaat
    24841 gatctcgctg ttatcaccgt tccagaacaa aggcaaagcg cagctggaag tgaaaatagg
    24901 cgaaaccaat ggtcgtctgg aaggcgatcg tgacaaagtg cgttttgtac agaccaatat
    24961 ggggcggttg attctggcag cccaaatgga tcgcactggt gccgactttg cggtgatgag
    25021 cggaggcgga attcgtgatt ctatcgaagc aggcgatatc agctataaaa acgtgctgaa
    25081 agtgcagcca ttcggcaatg tggtggtgta tgccgacatg accggtaaag aggtgattga
    25141 ttacctgacc gccgtcgcgc agatgaagcc agattcaggt gcctacccgc aatttgccaa
    25201 cgttagcttt gtggcgaaag acggcaaact gaacgacctt aaaatcaaag gcgaaccggt
    25261 cgatccggcg aaaacttacc gtatggcgac attaaacttc aatgccaccg gcggtgatgg
    25321 atatccgcgc cttgataaca aaccgggcta tgtgaatacc ggctttattg atgccgaagt
    25381 gctgaaagcg tatatccaga aaagctcgcc gctggatgtg agtgtttatg aaccgaaagg
    25441 tgaggtgagc tggcagtaat ccgaaagtgc cggatgtttg catccggcac aatgcttaat
    25501 cgcggcgggc gatatcagca aatttggcat cgaggatctt tgccagatcg cctgccgcca
    25561 gttcgatatc cagtccgcgc ttgccgccgg aaacataaat agtggcaaat tcttgtgcgg
    25621 gggcgtcgat aatcgttggc agacgttttt tctgccccag tgggctaatc cccccaacca
    25681 ggtatcccgt cgaacgctgc gcggccatcg gatcggccat ctcaactttc ttggcaccca
    25741 gcgcttttgc tactttttta agatccagtt gacctgcgac cggcgtaacg gccacggcaa
    25801 ggtgtttcat atcaccgttc actgccacca gcagcgtttt gtagacctga tccggattca
    25861 aacctaattt tttgacgact tcatcgccaa aattggtttc agccggatcg tgctcgtagg
    25921 tatggatttg aaacgaaatc ttgttttttt cgagtaattt aactgcgggt gtcatggtaa
    25981 ttttcctgcc ttaaacaaaa aaacgaagca agcatacgcc tgatggcggt ccaaaaaaaa
    26041 gagtcatctt gcctaagagt attggcagga tggtgagatt gagcgacaat cgagttacac
    26101 cgtcgcttaa agtgacggca taataataaa aaaatgaaat tcctctttga cgggccaata
    26161 gcgatattgg ccattttttt agcgcaacat ttgcggcaaa ttcccttctc catacaggtg
    26221 tagtgcaccg accgcgacca catatcgccc cggcggcatg gcgcgtaatt tatcccgcca
    26281 ggcgagattt cgctgatgca tcagcacatc gtacagcgac tgactgaacg tattgggcag
    26341 cgttatatca ttattttgcg gaggtgcatt cagccaccag ctcatcattt gttgcagcaa
    26401 ccgtgcgttg gtatgccagt gggtcagcgt atcgtccagc agcgccagtc ctttgtcagg
    26461 gagctggagc aacatggcaa tctggttttc agccccttcc agttcaatca cgggtttatg
    26521 ttgttgcttc gccgcctgca atagctggta atcgataccg tattctgccc gcagccccag
    26581 tttttgtgcc tgcgtcgcct gaagaaccat cgcgatttgc cacagcggtt gggtagaaaa
    26641 gagtgaggga gaaatgccca tctcctggct aatgtgctgc aggttttgga gttgctcctc
    26701 gctaatgcgc tcttccagcg cctcgcaggc aggcaaatta gcaaaaggcg tatcgctggt
    26761 ggaaacatcc gcctcgacga tcagcgcatc ggcgtttttg agctttttga gcaaacgggt
    26821 gggcagggga gccatatcgt ggctacccat atgaatactg ccaatcagat gaaaatggcg
    26881 attgccgggg agggtgatat cgatggcagg ccaggagtaa tgattaccgc gcagcgcggc
    26941 ccaaagcgtt tttacccggt acaacagatc catacgacct ccctttgtga aatatcatgc
    27001 tagcgcgcgg tgagggatgg cgcaaatgga gttggggact tgtttaatgt gtttgtatga
    27061 ttcagtatgt tcttgcatcg ctattcacag ggaagcaaca gttaaaaacc atgaaacagg
    27121 caacaagaaa accgacgaca cccggagata ttcttctcta tgaatatctg gaaccgctcg
    27181 atttgaaaat caatgagtta gcagagttgc tgcatgttca tcgtaatagc gtcagtgcac
    27241 tgatcaataa caatcgtaaa ctcaccactg agatggcatt ccgtctggcg aaagtttttg
    27301 ataccacagt cgattttagg ctaaacctcc aggcggcagt tgatctttgg gaagttgaaa
    27361 acaacatgcg cacccaggaa gaattgggac ggattgaaac agtggctgaa tatttggcac
    27421 gccgtgaaga gcgtgcaaaa aaggtcgcgt aaggccaaaa catcgcttca tctcgtaggc
    27481 cggataaggc gtttacgccg catccggcag tcatgcgtcg atgccaaatg cgccgcccta
    27541 aagcagcgca tccgcaatga cgtacttatt ccttcggttt aaaccgcagc aaccggttgg
    27601 cgttactcac tacggtaatc gacgagagcg ccattgccgc tccggcaact accgggttaa
    27661 gcagtgttcc agtaaacggc cacaaaatac cggcggcgac cggaataccg atgctgttgt
    27721 agataaaggc accgagcagg ttctgcttca tgttgcgcag cgttgcgcgg gaaatcgcga
    27781 gcgcatccgc aacgcccatc aggctatggc gcatcagggt aatcgccgcg gtttcaatgg
    27841 caacatcact gccgccaccc atcgcaatgc cgacatccgc ctgggccagc gctggcgcat
    27901 cgttaatgcc gtcgcccacc attgccacct gacgtccttc actttgcagg cgtttaatcg
    27961 cttcggcttt accgtccggc agcaccccgg cgatcacttc atcaatcccg gcttctttgg
    28021 cgatcgcatt ggcggtggtt gggttatccc cggtcaacat caccagacga tatcccgctt
    28081 tatgcaggcg ttgcagcgcc gccacgctat cactacgcaa tggatcgcgt actgccagca
    28141 gggctactgc tttcccgtca atcgccagca gcacaggcgt tgctccttgc gatgcctgag
    28201 cagtaatctc cgcttcgata gctttggtac caacctgttg ctcatttaac agcgcctgat
    28261 tgcccagcaa taacgcatga ccttcagctt caccgctcac gcccagcccg cgcaatgtgc
    28321 ggaaaccgtt gacctgcggc agctgcatat cacctgcttt atcgaggatc gcccgtgcca
    28381 gcggatggct ggaaccttgc tccagtgccg ccgccagacg caatgcctgc gcttcatcaa
    28441 catcagcaaa cgttttcact gcgacaacct gcggcttccc ttcggtcagc gtcccggttt
    28501 tatcgaacac gacggtatcg agtgtactgg cgcgttgcag cgcgtcggcg tcccgcacca
    28561 gcacgccaaa ctcagccgcc cgcccgacgc cggaaataat cgacatcggc gtcgccagcc
    28621 ccagcgcaca cggacaggcg ataatcagca ccgtggtggc tatcaccagg gtatagacaa
    28681 tctgcggtgc cggaccaaag aaataccaga ttgctgcact gatcagcgca atcaccacca
    28741 ccacaggtac aaatacggct gagattttat ccgccagctg accgatttct ggcttgctgc
    28801 tctgggcctg gcgcaccatg cgaatgatcc gtgacagcgt ggtatggctg ccaaccgcac
    28861 tggcgcgaaa tagcacgcta ccgtcctgta ccactgtccc ggcatggacg cttttgcctt
    28921 cgcctttttg ctgcgggatt ggttcgcccg tcagcatcgc ttcatccagc catgcttcgc
    28981 cctgggtaat ctcgccatct accggcacgc gatcgccggt cgtcaggcgc agcaacatac
    29041 ctggctgcac ttccgccaga ggcacgcttt tttcaccttc gtcagtaacc aggcgcgccg
    29101 tcggtggggt taaatcgagt aatttctcca gcgccttaga agaacgctga cgtgcgcgtg
    29161 cttccagcat atggccgaga ttgatcagac cgataatcat cgcgctggct tcgtaataaa
    29221 gatgtcgcgc ttccatcggg aaccactgcg gccacaggtt gacgctcatc gaatagagcc
    29281 acgccacgcc agtacccagc gccaccagcg tatccatcgt cgctgcaccg ttcagcaggc
    29341 ttttccatgc actgcggtaa aaatggccgc cggcgaaaac catcaccgcc agggttatca
    29401 ggccgataac caaccacagg ctgcggttgt cagcggtgac catcatgtta tcgccaatca
    29461 tcccccagac catcaccggg atacccaccg ccagtgcgac aattgcctgc cagcggaagc
    29521 gcttcatcgt agcaacggcg gtttcctgct ggcgctcacg gcgtttcgcg tcatcttcaa
    29581 tcgcttccgc gccgtagccc gctttttcca ccgcctgcac taaatcttgt ggggaggcac
    29641 tgcccatcac cagcgcagta cgctccgcca ggtttacccg tgcctgagtg acgcccggta
    29701 cgctttgcag cgcattttgt acgcgggtga cacagctggc gcagctcata ccgctcagca
    29761 gcaactgctg gctgtcatcg tcatcggcgg tcgctgccgg aagcgcctca gaaaccgctg
    29821 tcagtgcttc cgacgggatt gatgactccg ccagcggttt agcctttggg tggcttacag
    29881 atgcgtcata acccgcttgt ttgatggttt cgatcagctg ttctgcactg gcagtcccgg
    29941 taacgtgcgc ttcagtgata gacacatccg cttgttccac atccgggcgc tgttcgagac
    30001 tttctttcac gcgtttaacg cagtgaccgc aggacaggcc gtccagggtc aggtcgatag
    30061 tttgtgacat aaaacactcc tttaagacag ttttgactgg ctgtgataaa ggttaaacct
    30121 tccagcaagg ggaaggtcaa gaaattaata aaccaggcga gtaaaagtcc gtaaagatta
    30181 aaaaattggc tcaatttgca tcaggattag acatttatct ctttgttttc ctgtagttaa
    30241 gttgcgggtg ctaagttaaa tcaggatgcc tgaaaatcgg caccggggtg aggaattacc
    30301 tcccgcatct ataaaaagga gttaacaaaa gatgttagat gcaaacaaat tacagcaggc
    30361 agtggatcag gcttacaccc aatttcactc gcttaacggc ggtcaaaatg ccgactacat
    30421 tccctttctg gcgaatgtgc ctggtcaact ggcggcagtg gctatcgtga cctgcgatgg
    30481 caaggtctat agtgcgggtg acagtgatta tcgctttgcg ctggaatcca tctcgaaagt
    30541 ctgtacgtta gcccttgcgt tagaggatgt cggcccgcag gcggtacagg acaaaattgg
    30601 cgctgacccg accggattgc cctttaactc agttatcgcc ttagagttgc atggcggcaa
    30661 accgctttcg ccactggtaa atgctggcgc cattgccacc accagcctga ttaacgctga
    30721 aaatgttgaa caacgctggc agcgaatttt acatatccaa cagcaactgg ctggtgagca
    30781 ggtagcgctc tctgacgaag tcaaccagtc ggaacaaaca accaacttcc ataaccgggc
    30841 cattgcctgg ctgctgtact ccgccggata tctctattgt gatgcaatgg aagcctgtga
    30901 cgtgtacacc cgtcagtgct ccacgctcct caatactgtt gaactggcaa cacttggcgc
    30961 gacgctggcg gcggggggag tgaatccgct gacgcataaa cgcgttcttc aggccgataa
    31021 cgtgccgtac attctggccg aaatgatgat ggaagggctg tatggtcgct ccggtgactg
    31081 ggcatatcgc gttggtttac cgggcaaaag cggtgtaggt ggcggtattc tggcggtcgt
    31141 ccctggtgtg atgggaattg ccgcgttctc accaccgctg gacgaagaag gcaacagtgt
    31201 tcgcggtcaa aaaatggtgg catcggtcgc taagcaactc ggctataacg tgtttaaggg
    31261 ctgatcatga tgaacacgga aggtaataac ggtaacaaac ccctcggtct atggaacgtc
    31321 gtttccatcg gcattggggc aatggtgggg gcggggatct tcgcgctgct ggggcaggct
    31381 gcgttgctga tggaagcctc gacctgggtc gcctttgctt ttggcggtat tgtggcgatg
    31441 ttttccggtt atgcctatgc gcgtctgggg gcgagctatc ccagcaatgg cggcattatc
    31501 gacttctttc gtcgcggatt aggcaacggc gtcttttcgc tggcgctctc gttactgtac
    31561 ctgttgacgc tggcggtgag catcgccatg gtcgcccgtg cttttggcgc ttatgccgtg
    31621 cagtttttgc atgaaggcag ccaggaggag caccttattt tgctctacgc gttggggatc
    31681 attgcggtga tgacgctttt caactcctta agcaaccatg cggtagggcg gctggaagtg
    31741 atcctcgtcg gcattaaaat gatgatcctg ttattgctga ttattgccgg tgtctggtcg
    31801 ctgcaaccgg cgcatatttc cgtctctgcg ccccccagat ccggtgcgtt cttctcctgt
    31861 atcgggataa ccttccttgc ctatgcgggc tttggcatga tggcgaacgc ggcggataaa
    31921 gtgaaagatc cgcaggtcat tatgccgcgg gcgtttctgg tggcgattgg cgttaccacg
    31981 ttgctttata tctcgctggc actggttttg cttagcgatg tatcggcatt agagttagaa
    32041 aaatatgccg ataccgccgt agcgcaggct gcttctccgc tgctcggaca tgtgggttat
    32101 gtgatcgtcg tcatcggcgc tttactggcg acggcttcag ccattaacgc gaacctgttc
    32161 gccgtgttta acatcatgga caacatgggc agcgaacgcg aactgccgaa gctaatgaat
    32221 aaatccctgt ggcggcagag tacctggggc aacatcattg tcgtggtgtt gattatgctg
    32281 atgacggcgg cgctaaattt aggctcactc gccagtgttg ccagcgccac ctttttgatt
    32341 tgctacctgg cggtgtttgt ggtggcgatc cgcctgcgtc atgatattca cgcctcgttg
    32401 ccgattctta tcgttggtac gttggtgatg ttgttggtga tcgttggctt tatctacagt
    32461 ctgtggtccc agggtagccg tgcgttgata tggattattg gctcactctt actcagcctt
    32521 attgtggcaa tggtcatgaa gcgcaataaa accgtataac atctctctgt gcgcagtact
    32581 tcctgtatta ttgtggtggc ggtcgatatt cgcactggca aaaaaacgtg cttgaatatc
    32641 tgttgaaacc ctttaacaaa gcacaggagg cgttgcgcga acgatgaaca tcagcgatgt
    32701 agcaaaaatt accggcctga ccagcaaagc cattcgcttc tatgaagaga aggggctggt
    32761 gacgccgccg atgcgcagcg aaaacggtta tcgcacctac acgcagcagc atctcaacga
    32821 actgacctta ctgcgtcagg cacggcaggt gggctttaac ctggaagaga gcggcgagct
    32881 ggtgaatctg tttaacgacc cgcagcggca cagcgccgac gtcaaacggc gcacgctgga
    32941 gaaggtggcg gagatcgaac gacacattga ggagctgcaa tccatgcgcg accagctgct
    33001 ggcactggcg aatgcctgcc ctggcgatga cagcgccgac tgcccgatta tcgaaaatct
    33061 ctccggctgc tgtcatcatc gggcagggtg attaagacga gacggctcgg atatgtaggg
    33121 ttatcccttc tatcgcaatg acttcaacat gcgtacctgc accgagatcc tcgctggcgc
    33181 tgacaggcca tgaactgtca ccgacgcgca tatgaccgcg cccgttgacc agcggagatt
    33241 ccagcacaaa acgtcggcca atcagctgct gcccgcgctg gtttaaatga ctatcgctgt
    33301 gcttttgttc gcgcacccgc cgcgacaacc atttccacca cagccaggcg gcgagcagcg
    33361 tcaggatggc aaacattacc ccttgccact cccaacccag cggcaccagc cagaccacca
    33421 ggccggtaat caccgctgcc acgccgctcc acaacaaata accatttccg cccagcatct
    33481 cggctgccag cagcaaaccg ccgagactga gccagaaaat atgtggatga acgaccatta
    33541 actccatcat ggctgagtcc gtttgttggc gctgtctttc accagctcgg caatcccggc
    33601 aatcgacccc atcaggctgc tggcctctaa tggcatcatc actactttgc tgttactgga
    33661 ggaaccgatc tgctgtaacg cttcggtgta tttctgcgct acgaagtagt tcaccgcctg
    33721 aatatcaccg gaggcgatgg cttcagacac cattttggtg gcgcgggctt ctgcttcagc
    33781 ggaacgttca cgcgcttcag cctgtaaaaa cgccgactga cgttcgcctt ccgctttcag
    33841 gatttgcgac tgtttttcac cttcggcttt gaggatttcc gcctgacgga tcccttccgc
    33901 ttcaagaatg taagcgcgtt tggtacgttc cgctttcatc tgcgcgttca ttgaagagat
    33961 aagctctgcc ggtgggcgca cgtcgcgaat ttcaatacgg gtgactttaa tcccccacgg
    34021 gttggtggct tcatcgacaa tacgcagcag gcgtgagttg atgctgtcgc gctgagagag
    34081 catttcgtca agttccattg aacccaacac ggtacggatg ttagtcatgg tcaggttgat
    34141 gatcgccagc tccagattgc tgacttcata agccgcgcgc ggcgcgtcaa tcacctgaat
    34201 aaaacagact gcgtcgatgg taacgttggc gttatctttc gagataactt cctgggaagg
    34261 gatatcgagc acttgctcca tcatattgat cttgcgacca atgcgatcca taaacggcac
    34321 caccagactg agccccggct gtaacgtttt ggtatagcga ccaaaacgtt ctaccgtcca
    34381 ttgatagccc tgcggcacga ttttgacgcc cgcgccgaca atgaccagcg cgacaaaaat
    34441 gagaatcggg ataaagataa gcatcggaaa aacctcctgt tgtaccgtcc ataatcagca
    34501 aaattgctgc ttgattaaac aaattatacc tgattactga aagagagttc ccccttattc
    34561 ctgcgaagga taaactgttt ttagtaaaaa tcagaaaaag ggaacagcga tgcaggaaaa
    34621 tagtcctttg cttcagctac aaaacgtagg atatctggcg ggtgatacga agattcttaa
    34681 taacatcaat ttttcgctgc gtgctggcga atttaagtta attaccggtc cttctggttg
    34741 tggcaaaagt acgctgctaa aaatagttgc ttcattgatc agcccaacca gcggaacgtt
    34801 actgtttgaa ggggaggatg tcagcacact aaagccagaa atctaccgcc agcaagtctc
    34861 ttactgcgcc cagacaccga cgctgtttgg cgatacggta tacgataatc tgatctttcc
    34921 ctggcagatc cgtaaccagc agcctgaccc ggccattttt ctcgattttc tcgaacgctt
    34981 cgctttgccg gacagcattt tgacgaagga tatcgccgag ctatctggtg gtgaaaaaca
    35041 acgcatctca ttgattcgta acctgcaatt tatgccgaag gttttattgc tggatgaaat
    35101 aaccagtgcg ctggatgaaa gtaataaaca taacgtcaat gagatgatcc atcgttatgt
    35161 gcgcgagcaa aatattgccg tgctgtgggt gacacacgat aaagacgaaa ttaatcatgc
    35221 ggataaagtg attacactgc aaccgcatgc cggagaaatg caggaagcac gctatgaact
    35281 cgcataatat tactaacgaa tcattagcac tggcattaat gctggtggtg gtggcaatct
    35341 taattagcca taaagaaaaa ctggcgctgg agaaagatat tctctggagc gtcgggcgag
    35401 cgataattca gctgattatt gtcggctatg tgctgaagta tattttcagc gtggatgatg
    35461 ccagcctgac attattgatg gtgttattta tctgctttaa tgcggcgtgg aacgcgcaaa
    35521 aacgcagtaa atatattgct aaagctttta tctcatcgtt tgttgccatt acggtcgggg
    35581 cgggaattac cctggcggtg ctgattctct cagggtcgat tgaatttatc ccgatgcagg
    35641 tgatccctat cgccgggatg attgccggta acgccatggt agcggtgggg ttgtgttaca
    35701 acaatttagg gcaacgggtc attagcgaac agcaacagat tcaggagaaa ctgagtcttg
    35761 gtgcgacgcc gaagcaggct tcagcgatat tgattcgcga cagtattcgc gcggctttaa
    35821 ttccgacggt cgattcagca aaaacggttg gcttagtgag tttaccagga atgatgtccg
    35881 ggctgatatt tgccgggatt gatccggtga aggcgattaa atatcagatt atggtgacct
    35941 ttatgctgct ctcaaccgcc agcttgtcga ccattattgc ctgctattta acctatcgta
    36001 agttttataa ttcgcgccac cagttggtgg tgacgcaatt gaagaagaaa taatacgatg
    36061 ccggatgcgc agcatcgcat ccggcgttgt ggttcatgtg ccgatcaacg gaatatcaat
    36121 acaacaatgc atacagctgg cggcgatact tcgacgccag tgcatcaccc gtacccagtg
    36181 cagcgaggat ctcctggaac gttttacgcg tctgaccgtc tgcggcggtg agatctttac
    36241 gcagatgccc gaacagcaac tccagcgcct cttcattgcg cccaacctgg tgcagttgca
    36301 gagccagttg cgtcgccagt gcggcatctt ctggattctc cgccacctgc tgttgcaact
    36361 gttgaatttc cggcgtatca gccgcctgct tcagcagttc gatttgcgcc accagcccct
    36421 ggtagcgggt gtcctgatcc tgcaacggaa tggttttcag caccgcttcc gcatcttcag
    36481 aacggttcag cgcaatcagc gtttctgcca gcagcaggcc gatctccccg ttctgattcg
    36541 acaactgcca ggcgtctttc agcaatggca gggcatcggt gtaattgcct tcctgcatca
    36601 gttgcatcgc ctgctgcgct ttcagctctt cttcgcgcgg cagcacttta tccagcaggg
    36661 cgcggatcgc ctcttccggt tgcggcccct ggaagccatc taccggttgc ccgttctgga
    36721 acagatacac ggtcggaatc gcgcgcagac caaactgggc ggcaatcatc tgctccgcgt
    36781 cgcagtccag cttcgccaga ataaattgcc cgttgtactg cgccgcgagg ctttccagga
    36841 tcggggttaa ctgcaaacag tgctggctac gttcagacca aaaatagaac agcaccggag
    36901 tggtcatcga ctgttcaaga acctgttgca ggttagattc gttaatgttg acaatatttt
    36961 ctacggacat ggagtcgctc tctgttgtcg atattttctt tgacatgggg gcttaagcgc
    37021 gcgcttcaac tcacccctgc aatattttgt ccatcacgcg ccccggcagc aggcgcttaa
    37081 gcaccattac tgcccaggtc accagcgtta ccggatagcg catcttcggc ttctcgctaa
    37141 taaaagcatg gcgtactttg tccaccaccg cttccggtcc caacgtaaag cgggcggcga
    37201 tgccgggatt ttcgactggt ttatcacttt gcgtctggtt gacgttgtcg gtgaagcgag
    37261 tacgaatggg accgggttcg atcaggctga ctttaattcc gctgtggcgt agctccatgc
    37321 gcagcgcgtc tgaccacgcc tccagcgcat atttactggc cgcgtaagcg ccacgacccg
    37381 gcgtggagat taatcccatc accgatgatg tcatcacaat acgcccttca ccgtgcggta
    37441 acatcgcggg taacaggcgc atggtgagct ggtgtgcgcc gaaaaagttg gcggaaaact
    37501 gctgttccat ctgcgcacgg ctgatggtgg aaagggggcc atacatgccg aatccggcat
    37561 tgttaaagat cccatacaga caattatcgg tcagggcgat cacttcgtcg gctgcgcgat
    37621 caacactttc tggcgaatcc agatcgatca acacgccggt aaatcccatg ctgttcatac
    37681 gctcaacatc atccggtttc cggcaacctg ccagcacatg aaaaccctgg cgttttaatt
    37741 cgagcgcgct ttccaggcca attccactgg aacatccggt aattaagacc gatttttgca
    37801 taactttacc tgtcaggatc tccgttgctt tatgagtcat gatttactaa aggctgcaac
    37861 tgcttcgcca tccagtcggc aataaacggc tgggcgtcgc ggttgggatg aataccgtca
    37921 tcctgcatcc attgtggctt gaggtagacc tcttccataa aaaagggcag cagcggaaca
    37981 tcaaactctt tggcgagttt ggggtaaatg gcgctaaagg cttcattata acggcgacca
    38041 tagtttgcag gcagacgtat ttgcattaac aatggttcag cgttggcggc tttgacatcc
    38101 tgcaaaatct ggcgcagcgt ttgctcggtt tgctgtggct gaaaaccacg caaaccgtca
    38161 ttgccgccca gttcaaccag cacccaacgc ggctgatgct gtttcagcag agccggaagg
    38221 cgcgccagtc cttgttgcga ggtgtcgccg ctgatgctgg cattaactac cgacgtttta
    38281 ctctgccact tatcattcaa caaggcaggc caggccgcgc tggcagacat tcgatacccg
    38341 gcgctcaggc tatcacccag aatcaataac gtgtccgctg cggcggcacg gaaggttaac
    38401 aggaccagga acaggaaggg caaatgccag cggaaaacat tgttgaagtt catcatctta
    38461 agaagtccgt cggtcagggg gagcatgaac tctccatcct caccggagtt gagctggttg
    38521 tcaaacgtgg cgagaccatc gcactggtgg gcgagtcggg atcgggtaag tcaaccttgc
    38581 tggcgatcct cgccgggctt gatgacggca gcagtggcga agtgagtctg gtgggacaac
    38641 cgctacataa tatggacgaa gaagcgcggg caaagttgcg cgcgaagcac gtcggctttg
    38701 tttttcagtc atttatgtta attcctaccc ttaacgcgct ggaaaacgtc gagcttccgg
    38761 ctctgctgcg cggtgagagt agcgcggaaa gtcgtaacgg ggcgaaagcg ttgctcgaac
    38821 agttagggct gggtaaacgt ctggatcatc ttccggcaca gctttccggc ggtgaacagc
    38881 aacgagtggc gctggcacga gcctttaatg gtcgacctga tgtgctgttt gccgacgaac
    38941 ccaccggcaa ccttgaccgc cagacgggcg ataaaattgc cgacctgctg ttttccctca
    39001 accgtgaaca tggcaccacg ttgattatgg tgacccacga cctgcaactg gcggcacgct
    39061 gcgaccgctg cttacggctg gtgaacgggc agttgcagga ggaagcatga ttgcacgttg
    39121 gttctggcgc gaatggcgtt cgccgtcgct attaattgtc tggctggcgc taagcctggc
    39181 ggtggcctgc gtgctggcgc tgggcaatat cagcgatcgc atggagaagg gcttaagcca
    39241 gcaaagccgt gagtttatgg cgggcgatcg ggcgttgcgc agttcacgcg aagtgccgca
    39301 agcgtggctg gaggaagcgc aaaagcgcgg cctgaaagtc ggcaagcagc tgactttcgc
    39361 cacaatgacc tttgcaggcg acacaccgca gctggcgaac gtcaaagcgg tggatgatat
    39421 ctacccgatg tatggagatc tgcaaactaa tccccctggc ctgaaaccgc aggcgggcag
    39481 cgtattgctg gccccacgcc tgatggcact gcttaacctg aaaacgggcg acaccattga
    39541 cgtgggcgat gccaccttgc ggattgccgg agaagtgatt caggaaccgg attccggttt
    39601 taaccccttc cagatggctc cgcgtctgat gatgaatctg gcggatgtcg ataaaaccgg
    39661 agccgtgcag ccggggagtc gggtcacctg gcgttataaa ttcggcggca acgagaacca
    39721 gctcgacggc tatgagaaat ggttgttacc tcagcttaaa cccgaacaac gctggtacgg
    39781 tctggaacag gacgaaggcg cgctggggcg atcgatggaa cgctcgcaac agttcctgct
    39841 gctttcggcg cttctgacct tgctgctggc agtggcagcg gtggcggtag cgatgaatca
    39901 ttactgtcgc agtcgctacg atctggtggc gatcctcaaa acgctggggg cagggcgagc
    39961 gcaactgcgt aagctaatcg tcggtcagtg gttgatggtg ctgacgcttt cagccgttac
    40021 cggtggggcc ataggtctgt tgttcgaaaa cgtgttgatg gtgctgctca agcccgttct
    40081 gcctgctgca ctaccgccag ccagcctctg gccgtggctg tgggcgcttg gcaccatgac
    40141 ggtcatctcg ctgctggtgg ggctacgacc ataccgcttg ttgctggcaa cgcagccttt
    40201 acgcgtatta cgtaatgatg tggtagcgaa cgtctggccg ctgaagtttt atctgccgat
    40261 tgtcagtgtg gtggttgtgc tgctgctcgc cggattaatg ggtggcagca tgctgctttg
    40321 ggcggtgctg gcgggcgcgg tagtactggc tttgctgtgc ggtgtgctgg gctggatgct
    40381 gctgaatgta cttcgccgca tgacgctgaa atcgctgcct ctgcgcctgg cggttagccg
    40441 cctgttacgt cagccgtggt caacgttaag tcagctttcg gcattttcgc tctcctttat
    40501 gctgctggcg ctgttgctgg tattgcgtgg cgatctgctc gaccgctggc agcagcagct
    40561 accgccagaa agcccgaact actttttaat taacatcgcc acagaacagg tagcaccgct
    40621 aaaagcgttc ctcgcggaac atcatatagt cccggaatca ttttatccgg tagtgcgggc
    40681 gcggctgacg gcgattaacg ataagccgac agaaggtaat gaagatgagg cgcttaatcg
    40741 cgaactcaat cttacctggc aaaatacgcg gcccgatcat aatccgattg tcgccggtaa
    40801 ctggccgcca aaagccgatg aagtgtcgat ggaagagggg ctggcgaaac gcttaaacgt
    40861 tgccctcggt gataccgtga cttttatggg cgatacccag gagttccgcg ctaaagtgac
    40921 cagcctgcgc aaagtggact gggaaagtct gcggcctaat ttctatttta ttttcccgga
    40981 aggggcatta gacgggcaac cgcagagctg gcttaccagt ttccgctggg agaatggcaa
    41041 cggcatgttg acgcaactca accgccagtt cccgaccatt agcctgttag atattggcgc
    41101 gattttaaaa caggtcggtc aggtgctgga gcaggtaagt cgggcgctgg aggtgatggt
    41161 ggtgctggtc accgcctgcg gtatgttgct gttgctggca caggtgcagg tgggaatgcg
    41221 ccagcgtcat caggagctgg tggtatggcg cacactcggt gcggggaaaa aactgctgcg
    41281 cacgacattg tggtgtgagt tcgccatgct tgggtttgtt tccggcctgg tggccgcaat
    41341 tggtgcggaa acggcactgg cagtgttgca ggcgaaagtg tttgattttc cgtgggagcc
    41401 agactggcga ttgtggattg ttctgccgtg cagcggtgcg ctgctgctgt cgcttttcgg
    41461 cggctggctg ggtgcgcgac tggttaaggg taaggcgctg ttcaggcagt ttgcggggtg
    41521 atgagagtaa ataaggggcg ttctggttga atggaacgcc ttgtttaaat taattctaca
    41581 atatgtttca ttgatgatgt tattgaattg gt
//
LOCUS       DAPHDW010000026        40539 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.026, whole genome shotgun sequence.
ACCESSION   DAPHDW010000026 DAPHDW010000000
VERSION     DAPHDW010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40539
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.026"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            366..452
                     /gene="leuL"
                     /locus_tag="RH057_001369"
     CDS             366..452
                     /gene="leuL"
                     /locus_tag="RH057_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HDV3127765.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            545..2116
                     /gene="leuA"
                     /locus_tag="RH057_001370"
     CDS             545..2116
                     /gene="leuA"
                     /locus_tag="RH057_001370"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HDV3127766.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVANYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKAQHNENNKETV"
     gene            2116..3207
                     /gene="leuB"
                     /locus_tag="RH057_001371"
     CDS             2116..3207
                     /gene="leuB"
                     /locus_tag="RH057_001371"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HDV3127767.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLDAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDILCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAACAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            3210..4610
                     /gene="leuC"
                     /locus_tag="RH057_001372"
     CDS             3210..4610
                     /gene="leuC"
                     /locus_tag="RH057_001372"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HDV3127768.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAGLVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            4621..5226
                     /gene="leuD"
                     /locus_tag="RH057_001373"
     CDS             4621..5226
                     /gene="leuD"
                     /locus_tag="RH057_001373"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HDV3127769.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIAAYEAKQPAFMN"
     gene            complement(5275..6453)
                     /gene="setA"
                     /locus_tag="RH057_001374"
     CDS             complement(5275..6453)
                     /gene="setA"
                     /locus_tag="RH057_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HDV3127770.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLPSVARVELPSENALSMQGGWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSELGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVAAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNSISTGVILAGVIQGAIAQSWGHFAVYWVIAVISVVALFLTAKVKDV
                     "
     gene            complement(6555..6686)
                     /gene="sgrT"
                     /locus_tag="RH057_001375"
     CDS             complement(6555..6686)
                     /gene="sgrT"
                     /locus_tag="RH057_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HDV3127771.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            6775..8430
                     /gene="sgrR"
                     /locus_tag="RH057_001376"
     CDS             6775..8430
                     /gene="sgrR"
                     /locus_tag="RH057_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HDV3127772.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIA
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPREWETLSNFASHPIGTGPYAVIRN
                     TTNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEEKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      8464..8563
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            8594..9577
                     /gene="thiB"
                     /locus_tag="RH057_001377"
     CDS             8594..9577
                     /gene="thiB"
                     /locus_tag="RH057_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HDV3127773.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EADCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            9553..11163
                     /gene="thiP"
                     /locus_tag="RH057_001378"
     CDS             9553..11163
                     /gene="thiP"
                     /locus_tag="RH057_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HDV3127774.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            11147..11845
                     /gene="thiQ"
                     /locus_tag="RH057_001379"
     CDS             11147..11845
                     /gene="thiQ"
                     /locus_tag="RH057_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HDV3127775.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTNELL
                     SGKASASALLGITG"
     gene            complement(11947..12711)
                     /locus_tag="RH057_001380"
     CDS             complement(11947..12711)
                     /locus_tag="RH057_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3127776.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(12797..13675)
                     /gene="araC"
                     /locus_tag="RH057_001381"
     CDS             complement(12797..13675)
                     /gene="araC"
                     /locus_tag="RH057_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HDV3127777.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            14014..15714
                     /gene="araB"
                     /locus_tag="RH057_001382"
     CDS             14014..15714
                     /gene="araB"
                     /locus_tag="RH057_001382"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HDV3127778.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTHPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLSWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDAPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            15725..17227
                     /gene="araA"
                     /locus_tag="RH057_001383"
     CDS             15725..17227
                     /gene="araA"
                     /locus_tag="RH057_001383"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HDV3127779.1"
                     /translation="MTIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PCKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGDLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLAVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            17396..18091
                     /gene="araD"
                     /locus_tag="RH057_001384"
     CDS             17396..18091
                     /gene="araD"
                     /locus_tag="RH057_001384"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV3127780.1"
                     /translation="MLEDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQSGQSIPAIGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            18166..20517
                     /gene="polB"
                     /locus_tag="RH057_001385"
     CDS             18166..20517
                     /gene="polB"
                     /locus_tag="RH057_001385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HDV3127781.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IPADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDEAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDVIYGDTDSTFVWLKGAHSEEEAAKIG
                     RVLVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRETIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            20682..23588
                     /gene="rapA"
                     /locus_tag="RH057_001386"
     CDS             20682..23588
                     /gene="rapA"
                     /locus_tag="RH057_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HDV3127782.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKNCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            23600..24259
                     /gene="rluA"
                     /locus_tag="RH057_001387"
     CDS             23600..24259
                     /gene="rluA"
                     /locus_tag="RH057_001387"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HDV3127783.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(24376..25191)
                     /gene="djlA"
                     /locus_tag="RH057_001388"
     CDS             complement(24376..25191)
                     /gene="djlA"
                     /locus_tag="RH057_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HDV3127784.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQTGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            25446..27800
                     /gene="lptD"
                     /locus_tag="RH057_001389"
     CDS             25446..27800
                     /gene="lptD"
                     /locus_tag="RH057_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HDV3127785.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKLLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYRYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASWPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            27853..29139
                     /gene="surA"
                     /locus_tag="RH057_001390"
     CDS             27853..29139
                     /gene="surA"
                     /locus_tag="RH057_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HDV3127786.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATADIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            29139..30128
                     /gene="pdxA"
                     /locus_tag="RH057_001391"
     CDS             29139..30128
                     /gene="pdxA"
                     /locus_tag="RH057_001391"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HDV3127787.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRTYSPNSPAQPQTAGTLTLLPVALRESVTAGQLAVENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDAGIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPLLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            30125..30946
                     /gene="rsmA"
                     /locus_tag="RH057_001392"
     CDS             30125..30946
                     /gene="rsmA"
                     /locus_tag="RH057_001392"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HDV3127788.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDVRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            30949..31326
                     /gene="apaG"
                     /locus_tag="RH057_001393"
     CDS             30949..31326
                     /gene="apaG"
                     /locus_tag="RH057_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HDV3127789.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            31333..32175
                     /gene="apaH"
                     /locus_tag="RH057_001394"
     CDS             31333..32175
                     /gene="apaH"
                     /locus_tag="RH057_001394"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HDV3127790.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(32253..32732)
                     /gene="folA"
                     /locus_tag="RH057_001395"
     CDS             complement(32253..32732)
                     /gene="folA"
                     /locus_tag="RH057_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HDV3127791.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(32924..34786)
                     /gene="kefC"
                     /locus_tag="RH057_001396"
     CDS             complement(32924..34786)
                     /gene="kefC"
                     /locus_tag="RH057_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HDV3127792.1"
                     /translation="MDSHTLIQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLATSSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLLENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(34779..35309)
                     /gene="kefF"
                     /locus_tag="RH057_001397"
     CDS             complement(34779..35309)
                     /gene="kefF"
                     /locus_tag="RH057_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HDV3127793.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVFSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(35417..36748)
                     /locus_tag="RH057_001398"
     CDS             complement(35417..36748)
                     /locus_tag="RH057_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3127794.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMF
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGLLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLISSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMG"
     gene            complement(36806..37093)
                     /gene="fixX"
                     /locus_tag="RH057_001399"
     CDS             complement(36806..37093)
                     /gene="fixX"
                     /locus_tag="RH057_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HDV3127795.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(37090..38376)
                     /locus_tag="RH057_001400"
     CDS             complement(37090..38376)
                     /locus_tag="RH057_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV3127796.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVILAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGYPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(38427..39368)
                     /locus_tag="RH057_001401"
     CDS             complement(38427..39368)
                     /locus_tag="RH057_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV3127797.1"
                     /translation="MNTFSQVWVFSDTPSRLPELMNGAQALANQINTFVLNDADGVQA
                     IQLGANHVWKLNGKPDDRMIEDYAGVMADTIRQHGADGLVLLPNTRRGKLLAAKLGYR
                     LKAAVSNDASTVSVQDGKATVKHMVYGGLAIGEERIATPYAVLTISSGTFDAAQPDAS
                     RTGETHTVEWQAPAVAITRTATQARQSNSVDLDKARLVVSVGRGIGSKENIALAEQLC
                     KAIGAELACSRPVAENEKWMEHERYVGISNLMLKPELYLAVGISGQIQHMVGANASQT
                     IFAINKDKNAPIFQYADYGIVGDAVKILPALTAALAR"
     gene            complement(39383..40153)
                     /locus_tag="RH057_001402"
     CDS             complement(39383..40153)
                     /locus_tag="RH057_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HDV3127798.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPNELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
BASE COUNT         9787 a        11142 c        10629 g         8981 t
ORIGIN      
        1 tatatttaat gcattaattc ttaacattaa ttgatcaata atattcacca aatcaatatc
       61 aaaaaaaatc gcaaaacata taattcaata caaatcatca ggataggttt tgcaacgcgt
      121 gcattttgtc ccctttttcc tcgttgatta gatgcaaaaa tttatgctga aatatgtcaa
      181 ccgatgaaaa gcgtcggtag ttaagcagaa attaatatcg cttactttaa ccaccgcagc
      241 acaattagct aattttacgg atgcagaact cacgctggcg ggacgttttt attgcgtcag
      301 ggttgacatc cgtttttgta tccagtaact ctaaaagcat atcgcattca tctggagctg
      361 atttaatgac tcacatcgtt cgctttatcg gtctactact actaaacgca tcttctttgc
      421 gcggtagacg agtgagcggc atccagcatt aagccagcac gcagtcaaac aaaaaacccg
      481 cgccattgcg cgggtttttt tatgcccgaa gcgaggcgct ctaaaagaga caaggaccca
      541 aaccatgagc cagcaagtca ttattttcga taccacattg cgcgacggtg aacaggcgtt
      601 acaggcaagc ttgagtgtga aagaaaaact gcaaattgcg ctggcccttg agcgtatggg
      661 tgttgacgtg atggaagtcg gtttccccgt ctcttcgccg ggcgattttg aatcggtgca
      721 aaccatcgcc cgccaggtta aaaacagccg cgtatgtgcg ttagctcgct gcgtggagaa
      781 agatatcgac gtggcggccg aatccctgaa agtcgccgaa gccttccgta ttcatacctt
      841 tattgccact tcgccaatgc acatcgccac caagctgcgc agcacgctgg atgaagtgat
      901 cgaacgcgct atctatatgg tgaaacgcgc ccgtaattac accgatgatg ttgaattttc
      961 ttgcgaagat gccgggcgta cacccattgc cgatctggcg cgagtggtcg aagcggcgat
     1021 taatgccggt gccaccacca tcaacattcc ggacaccgtg ggctacacca tgccgtttga
     1081 gttcgccgga atcatcagcg gcctgtatga acgcgtacct aacatcgaca aagccattat
     1141 ttccgtacat acccacgacg atttaggcct ggcggtcggc aactcactgg cggcggtaca
     1201 tgccggtgca cgccaggtgg aaggtgcaat gaacgggatc ggcgagcgtg ccggtaactg
     1261 ttcgctggaa gaagtcatca tggcgattaa agttcgtaag gatattctca acgtccacac
     1321 cgccattaat caccaggaga tatggcgcac cagccagtta gttagccaga tttgtaatat
     1381 gccgatcccg gcaaacaaag ccattgttgg cagcggcgca ttcgctcact cctccggtat
     1441 ccaccaggat ggcgtgctga aaaaccgcga aaactacgaa atcatgacac cagaatctat
     1501 tggtctgaac caaatccagc tgaatctgac ctctcgttcg gggcgtgcgg cggtgaaaca
     1561 tcgcatggat gagatggggt ataaagaaag tgaatataat ttagacaatt tgtacgatgc
     1621 tttcctgaag ctggcggaca aaaaaggtca ggtgtttgat tacgatctgg aggcgctggc
     1681 cttcatcggt aagcagcaag aagagccgga gcatttccgt ctggattact tcagcgtgca
     1741 gtctggctct aacgatatcg ccaccgccgc cgtcaaactg gcctgtggcg aagaagtcaa
     1801 agcagaagcc gccaacggta acggtccggt cgatgccgtc tatcaggcaa ttaaccgcat
     1861 cactgactat aacgtcgaac tggtgaaata cagcctgacc gccaaaggtc acggtaaaga
     1921 tgcgctgggt caggtggata ttgtcgccaa ctacaacggt cgccgcttcc acggcgtcgg
     1981 cctggccacc gatattgtcg agtcctccgc caaagccatg gtgcacgtac tgaacaatat
     2041 ctggcgtgcc gcagaagtcg aaaaagagtt gcaacgcaaa gctcaacaca acgaaaacaa
     2101 caaggaaacc gtgtgatgtc gaagaattac catattgccg tattgccggg ggacggtatt
     2161 ggtccggaag tgatgaccca ggcgctgaaa gtgctggatg ccgtgcgcaa ccgctttgcg
     2221 atgcgcatca ccaccagcca ttacgatgta ggcggcgcag ccattgataa ccacgggcaa
     2281 ccactgccgc ctgcgacggt tgaaggttgt gagcaagccg atgccgtgct gtttggctcg
     2341 gtaggcggtc cgaaatggga acatttacca ccagaccagc aaccagaacg cggcgcgctg
     2401 ttgcctttgc gtaagcactt caaattattc agcaacctgc gcccggcaaa actgtatcag
     2461 gggctggaag cattctgtcc gctgcgtgca gacattgccg ctaacggctt cgacatcctg
     2521 tgcgtgcgcg aactgaccgg cggcatctat ttcggtcagc caaaaggccg cgaaggtagc
     2581 ggacaatatg aaaaagcgtt tgataccgag gtgtatcacc gttttgagat cgagcgtatt
     2641 gcccgcatcg cgtttgaatc tgctcgtaaa cgtcgccaca aagtcacctc aatcgacaaa
     2701 gctaacgtgc tgcaatcctc tattttatgg cgtgaaatcg tcaacgagat cgccacggaa
     2761 tacccggatg tcgaactggc gcatatgtac atcgacaacg ccaccatgca gctgattaaa
     2821 gatccatcac agtttgacgt tctgctgtgc tccaacctgt ttggcgacat tctgtctgac
     2881 gagtgcgcaa tgatcactgg ctcgatgggg atgttgcctt ccgccagcct gaacgagcaa
     2941 ggttttggtc tgtatgaacc ggcaggcggc tcagcaccag atatcgcagg caaaaacatc
     3001 gccaacccga ttgcgcaaat tctgtcgctg gcactgctgc tgcgctacag cctggatgcc
     3061 gatgatgcgg cttgcgccat tgaacgcgcc attaaccgcg cattagaaga aggcattcgc
     3121 accggggatt tagcccgtgg cgctgccgcc gttagtaccg atgaaatggg cgatatcatt
     3181 gcccgctatg tggcagaagg ggtgtaatca tggctaaaac gttatacgaa aaattattcg
     3241 acgctcacgt agtgtacgaa gccgaaaacg aaaccccgct gttatatatc gaccgccacc
     3301 tggtgcatga agtgacctca ccgcaggcgt ttgatggtct gcgcgcccac ggtcgcccgg
     3361 tacgtcagcc gggcaaaacc ttcgccacta tggatcacaa cgtctctacc cagaccaaag
     3421 acattaatgc ctgtggtgaa atggcgcgca tccagatgca ggagctgatt aaaaactgca
     3481 aagaatttgg cgtcgagctg tatgacctga atcacccgta tcaggggatc gtccacgtaa
     3541 tggggccgga acagggcgtc accttgccgg ggatgaccat tgtctgcggc gactcgcata
     3601 ccgccaccca cggcgcgttt ggcgcactgg cctttggtat cggcacttcc gaagttgaac
     3661 acgtactggc aacacaaacc ctgaaacagg gccgtgcgaa gaccatgaaa attgaagtcc
     3721 agggcaaagc cgcgccgggc attacagcaa aagatatcgt gctggcaatt atcggtaaaa
     3781 ctggcagcgc aggcggcacc gggcatgtgg tggagttttg cggcgaagca attcgtgatt
     3841 taagcatgga aggtcgtatg accctgtgca atatggcaat cgaaatgggc gcaaaagccg
     3901 gtctggttgc accggacgaa accaccttta actatgtcaa aggccgtttg catgcgccga
     3961 aaggcaaaga tttcgacgac gccgttgcct actggaaaac cctgcaaacc gacgaaggcg
     4021 caactttcga taccgttgtc actctgcaag cagaagaaat ttcaccgcag gtcacctggg
     4081 gcaccaatcc aggccaggtg atttccgtga acgacaatat tcccgatccg gcttcgtttg
     4141 ccgatccggt tgaacgcgcg tcggcagaaa aagcgctggc ctatatgggg ctgaaaccgg
     4201 gcattccgct gaccgaagtg gctatcgaca aagtgtttat cggttcctgt accaactcgc
     4261 gcattgaaga tttacgcgcg gcagcggaga tcgccaaagg gcgaaaagtc gcgccaggcg
     4321 tgcaggcact ggtggttccc ggctctggcc cggtaaaagc ccaggcggaa gcggaaggtc
     4381 tggataaaat ctttattgaa gccggttttg aatggcgctt gcctggctgc tcaatgtgtc
     4441 tggcgatgaa caacgaccgt ctgaatccgg gcgaacgttg tgcatccacc agcaaccgta
     4501 actttgaagg ccgccagggg cgcggcgggc gcacgcatct ggtcagcccg gcaatggctg
     4561 ccgctgctgc tgtgaccgga catttcgccg acattcgcaa cattaaataa ggagcacacc
     4621 atggcagaga aatttatcaa acacacaggc ctggtggttc cgctggatgc cgccaatgtc
     4681 gataccgatg caatcatccc gaaacagttt ttgcagaaag tgacccgtac gggttttggc
     4741 gcgcatctgt ttaacgactg gcgttttctc gatgaaaaag gccaacagcc aaacccggac
     4801 ttcgtgctga acttcccgca gtatcagggc gcttccattt tgctggcacg agaaaacttc
     4861 ggctgtggct cctcgcgtga gcacgcgccc tgggcattga ccgactacgg ttttaaagtg
     4921 gtgattgcgc cgagttttgc tgacatcttc tacggcaata gctttaacaa ccagctgctg
     4981 ccggtgaaat taagcgatgc agaagtggac gaactgtttg cgctggtgaa agccaatccg
     5041 gggatccatt tcgacgtgga tctggaagcg caagaggtga aagcgggaga gaaaacctat
     5101 cgctttacca tcgatgcctt ccgccgccac tgcatgatga acggtctgga cagtattggg
     5161 cttaccttgc agcacgacga cgccattgcc gcttatgaag caaaacaacc tgcgtttatg
     5221 aattaatccc cttgcccggt caaatgaccg ggctttccgc tatcgtccac gtcatcaaac
     5281 gtctttaacc tttgcggtta aaaataatgc gacaacagaa ataaccgcaa ttacccagta
     5341 gacagcaaag tgcccccaac tttgtgcaat tgctccctga ataacgccag ccagaattac
     5401 cccggtagaa atactgttag taaataaggt ggtagctgcc cccgctcttc caggcattaa
     5461 atcctgaaac catagcatcc caatacccgc aacaatgccg ataaatacag cgttaaaaag
     5521 ttgcagcgtc atcaacgcca tacggctatg aaagaaaatc aatccggtgt aaaacagtac
     5581 tcctgccgcc actgctatga ccatcattcg ccgcttacca taacgtttga catagtagcc
     5641 agccagaatc attgctggta tttccagtcc agctgccgtc cccatcagga aacccgccag
     5701 tttgtctggc aatcctaact cgctactgat ccacaacggc atatcaataa tgtacatggt
     5761 gttgcaggtc cacattaacg tcgaggcgac aaataacatc cgtacgttac tatcctgcca
     5821 gccaccttgc attgataaag cattttccga cggcagttct acccgcgcca cagacggaag
     5881 cataaatgca atcaatacca gactgagtgt gaatatcccg gcggcaatcg aaaacatcac
     5941 cgtaaagccg taattcaacg ccagcataaa ggccaacggt ggaccgataa cccatgccag
     6001 agaaagctgc gcacgcatca ccgagctaaa catcaccact tctcgcgccg agttatccgc
     6061 atattcccgc gccagagcaa ataactgtgg cattgccgta ttggccagag atgccagaag
     6121 cacaccacag gtgataagcg tcagataatg acgattaaat gcaaacaata gcgcattgcc
     6181 gatagccatc aaacagcaaa atataatcag ttttcgccga tcgccctgac tgtcagaacg
     6241 ttttgccaac cagaggctta cgccgatccc agcaatagca ttcaccgtat aaaagagtcc
     6301 gatccagaaa ggttgcgcgc caacctcacg actcagaaat aagctcaatg taggagcctg
     6361 tagcgccccg gccaccccca tcataaaagc gaccagcata aatgccgcgt aaacaccgtt
     6421 catacggcga gccatcgtca ttatccagat catacgttcc ctttttagcg cggcgagaat
     6481 aaaaaaaacc agcaggtata atctgctggc gggtgatttt acaccaatac tcagtcacac
     6541 atgatgcagg caagtcaact ttcagaattg cggtcatccc actgcatcag tccttcaagc
     6601 atggttaatc gttgtgggac gcttaaccaa cgcaaccagc acaacttcgc tgtcgcggta
     6661 aaatagtgct gataaaactg acgcatgggg cacctccttg cttcatcgtt aggaattatt
     6721 gacttaatat agggaaaata aaattgctgt cttttgcgca ggagttcccc ttttatgcca
     6781 tctgctcgtc tgcaacaaca gttcatccgc ctgtggcaat gctgcgaggg taaatcgcag
     6841 gacacaacgc tcaacgaact ggcagcgtta ttgagctgct cgcgtcgtca tatgcgtacc
     6901 ctgctcaaca ccatgcagga tcgcggctgg ctgacgtggg aagcggaagt cgggcgcggt
     6961 aaacgctcgc gtctgacatt cctctatacc gggctggcgc ttcagcaaca gcgggcggaa
     7021 gacctgctgg agcaggatcg tatcgaccaa ctagtgcagt tggttggcga caaagcgact
     7081 gtgcggcaaa tgctggtttc tcatctgggc cgcagcttcc gccaggggcg gcacatcctg
     7141 cgcgtgctct actatcgtcc gttgcgtaat ctgctacctg gcagcgcatt gcgccgttcc
     7201 gaaacccata ttgcccggca aatcttcagt tcgctaacgc gcataaatga ggaaaatggg
     7261 gaactggaag cagacatcgc ccaccactgg cagcaaatat caccgcttca ctggcgtttc
     7321 tttttgcgtc caggagtcca ttttcatcat ggtcgtgaac tggaaatgga cgacgtgatc
     7381 gcctctttaa aacgaatcaa tacgctgccg ctctattcgc atattgcgga cattgtgtcg
     7441 ccgacgccct ggacgctgga tatccatctc acgcaaccgg accgctggtt accgttactg
     7501 ctgggacaag ttccggcgat gatcctgccg cgcgaatggg aaaccctcag taactttgcc
     7561 agccatccca tcggcaccgg tccgtatgcg gtgatccgca acaccaccaa tcaactgaaa
     7621 attcaggcat tcgatgactt cttcggttac cgggcattaa tcgatgaagt taacgtctgg
     7681 gttctgccgg aaattgccga cgagccagct ggaggtctga tgctaaaagg tccacagggc
     7741 gaggaaaaag agattgaaag ccgcctggag gaaggttgct actatttact gttcgacagc
     7801 cgcacccatc gcggggcgaa tcagcaagtc agggactggg taagctatgt gctttctcca
     7861 actaatctgg tctatttcgc tgaggaacag taccagcaac tgtggttccc ggcttatgga
     7921 ctgctccccc gttggcacca tgcccgcacc ataaagagcg aaaaaccggc tggcctggaa
     7981 agcctcaccc tgacctttta tcaggatcac agtgagcatc gggtgattgc cgggatcatg
     8041 cagcagattc tggcaagtca ccaggtcacg ctggaaatca aagagatcag ctacgatcag
     8101 tggcatgaag gagagatcga gagcgatatc tggcttaata gcgccaactt tacgctgccg
     8161 ctggattttt cgctgttcgc gcacttgtgc gaggtgccgc tgctccaaca ctgtattccg
     8221 atcgactggc aagccgacgc cgcccgctgg cgcaatggcg aaatgaacct ggcgaactgg
     8281 tgccagcaac tggtcgccag taaagcaatg gtgccgctta tccaccactg gctgatcatt
     8341 cagggacaac gcagtatgcg cggcctgcgt atgaataccc tcggctggtt cgattttaaa
     8401 tcagcgtggt ttgcgccgcc ggatccatga ttgctggtag ataacaaaat cactacacta
     8461 acgccgttct caacggggtg ccacgcaaac gcgtgcgctg agaaaatacc cgtcgaacct
     8521 gatccggata acgccggcga agggatttga ggctccttct caagtccttt gccactcttt
     8581 tttgaggtgc aaagtgttaa aaaaatgtct tcccctgctg ttgctgtgca cagcgcccgt
     8641 tttcgctaaa cccgttctga ctgtttatac ctacgattcc ttcgccgccg actgggggcc
     8701 tggtccaaag atcaaaaaag cttttgaagc cgactgtaac tgcgaactga aactggtggc
     8761 gctggaagat ggcgtttcgc ttctcaaccg tctacggatg gaaggcaaaa atagtaaagc
     8821 cgatgtggtg ctggggctgg ataacaatct gttagacgcc gccagtaaaa ccggactatt
     8881 tgccaaaagc ggtgtggcag cggatgccgt taacgttccc ggcggctgga ataatgacac
     8941 tttcgtcccg tttgattacg gctatttcgc cttcgtctat gacaagaaca aactgaaaaa
     9001 cccgccgcaa agcctgaaag aattggttga gagcgatcaa aactggcggg tgatttatca
     9061 ggatccgcgc accagtacgc cggggctggg cctgctgttg tggatgcaaa aagtctatgg
     9121 cgatgacgcc ccacaagcct ggcaaaaact ggcgaagaaa accgtcacgg tcaccaaagg
     9181 ctggagcgaa gcctacggcc tgtttttaaa aggtgaaagc gatctggtac tgagttacac
     9241 cacctctccg gcttatcaca ttctcgaaga gaaaaaagat aactacgccg ccgcgaactt
     9301 tagcgaaggt cactatctgc aggtggaagt cgccgcccgc accgctgcca gcaagcagcc
     9361 ggagctggcg caaaaattcc tccagtttat ggtttctccg gcttttcaga atgcgatccc
     9421 aaccggtaac tggatgtatc cggtggcaaa tgtcacgctg cctgccgggt ttgaacaatt
     9481 gaccaaaccg gccaccacgc tggagttcac accagctgaa gtggcggcac aacgtcaggc
     9541 atggattagc gaatggcaac gcgccgtcag ccgttaattc ccggctggtt aattccaggt
     9601 gtaagcgccg ccacgctggt ggtagcggtt gcgctcgcgg cgtttctcgc cctgtggtgg
     9661 aacgcgccgc agggtaactg ggtggcagtc tggcaggaca gctacctgtg gcatgtggtg
     9721 cgcttctcct tctggcaggc gtttctctcg gcgctactat ctgtcgtacc cgcgatattc
     9781 ctcgcccgcg ccctctatcg caggcgcttt ccgggtcggc tggcgctgtt gcgtctgtgc
     9841 gcaatgacct tgatcctccc ggtgctagtt gctgttttcg gcattcttag cgtctatggt
     9901 cgtcagggct ggctggcatc gctctgccaa tcgctcggtc tggagtggac cttttcgccc
     9961 tacggcctgc aaggcatttt gctggcgcac gtatttttta atctgccgat ggcgagccgc
    10021 ttattactcc aggcactgga aaacattccc ggcgaacaac gtcagcttgc cgcccagctt
    10081 gggatgcgcg gctggcattt tttccgcttc gtcgaatggc cgtggctacg gcgacaaatc
    10141 ccgccggttg ccgcgcttat ctttatgctc tgtttcgcca gcttcgccac cgtgctatcg
    10201 ctgggcggcg gtccgcaggc aactactatc gagctggcta tttatcaggc gctgagttac
    10261 gactacgatc ctgcccgcgc ggcgatgctg gcgctgctcc agatggtgtg ttgccttggg
    10321 ctggtgctgt tgagtcagcg actaagtaag gccattgcgc ccggtaccac gctgctgcaa
    10381 ggctggcgcg atccggacga tcgtctgcat agccgcattt gcgacacggt gttaatagtg
    10441 ctggcgctgc tgctgttgct gccaccgttg ctggcggtga tcgtcgatgg cgtaaatcgc
    10501 cagttgccgg aagtgctggc acaaccggtg ctgtggcagg cgctgtggac ctcgttgcgt
    10561 attgcgctgg cggcaggtgt attgtgcgta gtgctgacca tgatgctgct atggagcagt
    10621 cgcgaactgc gggcgcggca gaaaatgctg gcgggtcagg cgctggagat gagcggcatg
    10681 ttgatcctcg ccatgccggg gattgtgctg gctaccggct tctttttact gctcaacaac
    10741 actatcggcc tgccacaatc tgctgacggc attgtgattt tcaccaatgc gttaatggcg
    10801 atcccttatg cgctgaaagt actggaaaac ccgatgcgcg atatcaccgc ccgctacagt
    10861 atgttgtgtc agtcgctggg cattgaaggc tggtcgcgct taaaagtggt cgagctgcgc
    10921 gccctgaaac gtccactggc gcaggcgctg gcttttgcct gcgtgctgtc gattggtgat
    10981 tttggcgtag tggcgttgtt cggtaacgat gatttccgta ccctgccgtt ttatctctac
    11041 cagcaaattg gctcctatcg cagccaggac ggcgcggtca ccgcgttaat tctgctacta
    11101 ctctgttttc tgctgtttac cgtgattgaa aaactaccgg ggcgaaatgt taaaactgac
    11161 tgatatcacc tggctttatc accatttgcc gatgcgtttt agcttaacgg tagaacgcgg
    11221 cgagcaggtg gcaatcctcg ggccaagcgg cgcgggtaaa agtaccctgc tgaatttgat
    11281 tgccggtttt ctgacgccag ccagcggttc gctgactatc gacggcgttg atcacaccac
    11341 gacgccgccg tcacgccgtc cggtatcgat gctgtttcag gagaacaacc tgttcagcca
    11401 cctgacggtc gcgcagaaca tcgggctggg gcttaatccg ggattgaagc tgaacgcggc
    11461 gcagcaggag aaaatgcacg ctatcgcccg ccagatgggg attgataatt taatggcgcg
    11521 gttaccgggc gagctttccg gcggtcagcg acagcgcgtg gcgttagcgc gttgtctggt
    11581 gcgcgaacag ccgattttat tgctcgatga accgttctct gcgctcgatc cggcgttacg
    11641 tcaggagatg ctgacgctgg tgagcacgag ctgccagcag caaaaaatga cgctattgat
    11701 ggtgtcgcac agtgtggaag atgcggcgcg gatcgccaca cgctcggtgg tggtcgccga
    11761 cgggcgcatc gcctggcagg gtaagaccaa tgagttgttg agcggtaagg cgagtgcttc
    11821 ggcactattg gggattacgg gttagtatgc actgtcggat gcggcgtgaa cgccttatcc
    11881 gacctacggg tagtgcgttt tgtaggcctg ataagacgcg gcaagcgtcg catcaggcaa
    11941 cccgccctaa accccaacca ctttacgcaa aatatcgata tacaccggca tcaacgggtg
    12001 gcgaattaac accaccagcg ccaccacgcc gatggcagaa atcaacggcg tcagccacaa
    12061 caaacgaccg cgggacaaat aatgactcaa acggtcagtc gctttaccgc tgcgccataa
    12121 ccgccagcac agccagccac caacccacaa aaataccgct gttgccagca gcaaccattt
    12181 aaactcaccg ctctgcattc cggcaggaat atcgatcgcc gcgcccgcca gaatccccgg
    12241 caggaagtaa aacggcggcc ataacaggca gccaataata tttggcgtga taaattttgc
    12301 caccggcaga tccagcattc ccgccaccat tggcaccagc ggacgcgtcg ggccaacaaa
    12361 gcgaccgacc agaatggtga acatgctgtg ttgatgcaac gcatgttcag ttttatcgag
    12421 taatgctttg tttttcttca gaaatgacca gcgatgcaac ggctttttaa aacgccagcc
    12481 cagccagaag gagatccagt cgcccagcag acaaccgacg atccctgcca gccaggcgtg
    12541 ccagaaactt aactcgccgc tgccaatcag cgctcccagc cccgccatca gcaccgtacc
    12601 gggtagaatc aaaccgacca gcgccagcga ctccagaaag gccaccaaca ccaccgccat
    12661 caatgaatac acggtagatt gggtaataaa gtgttccagc aatgcttgca taatgtgcct
    12721 gtcaaatgga cgaagcaggg attctgcaaa ccctatgcta ctccgtcaag ccgtcaattg
    12781 tctgattcgt taccaattat gacaacttga cggctacatc attcactttt tcttcacaac
    12841 cggcacggaa ctcgctcggg ctggccccgg tgcatttttt aaatactcgc gagaaataga
    12901 gttgatcgtc aaaaccaaca ttgcgaccga cggtggcgat aggcatccgg gtggtgctca
    12961 aaagcagctt cgcctggctg atacgttggt cctcgcgcca gcttaagacg ctaatcccta
    13021 actgctggcg gaaaagatgt gacagacgcg acggtgacaa gcaaacatgc tgtgcgacgc
    13081 tggcgatatc aaaattgctg tctgccaggt gatcgctgat gtactgacaa gcctcgcgta
    13141 cccgattatc catcggtgga tggagcgact cgttaatcgc ttccatgcgc cgcagtaaca
    13201 attgctcaag cagatttatc gccagcagct ccgaatagcg cccttcccct tgcccggcgt
    13261 taatgatttg cccaaacagg tcgctgaaat gcggctggtg cgcttcatcc gggcgaaaga
    13321 aacccgtatt ggcaaatatt gacggccagt taagccattc atgccagtag gcgcgcggac
    13381 gaaagtaaac ccactggtga taccattcgc gagcctccgg atgacgaccg tagtgatgaa
    13441 tctctcctgg cgggaacagc aaaatatcac ccggtcggca aacaaattct cgtccctgat
    13501 ttttcaccac cccctgaccg cgaatggtga gattgagaat ataacctttc attcccagcg
    13561 gtcggtcgat aaaaaaatcg agataaccgt tggcctcaat cggcgttaaa cccgccacca
    13621 gatgggcgtt aaacgagtat cccggcagca ggggatcatt ttgcgcttca gccatacttt
    13681 tcatactccc gccattcaga gaagaaacca attgtccata ttgcatcaga cattgccgtc
    13741 actgcgtctt ttactggctc ttctcgctaa cccaaccggt aaccccgctt attaaaagca
    13801 ttctgtaaca aagcgggacc aaagccatga caaaaacgcg taacaaaagt gtctataatc
    13861 acggcagaaa agtccacatt gattatttgc acggcgtcac actttgctat gccatagcat
    13921 ttttatccat aagattagcg gatcctacct gacgcttttt atcgcaactc tctactgttt
    13981 ctccataccc gtttttttgg atggagtgaa acgatggcga ttgcaattgg cctcgatttt
    14041 ggcagtgatt ctgtgcgagc tttggcggtg gactgcgcca gcggtgaaga gatcgccacc
    14101 agcgtagagt ggtatccccg ttggcaaaaa gggcaatttt gtgatgcccc gaataaccag
    14161 ttccgtcatc atccgcgtga ctacattgag tcaatggaag cggcactgaa aaccgtgctt
    14221 gcagagctta gcgtcgaaca gcgcgcagct gtggtcggga ttggcgttga cagtaccggc
    14281 tcgacgcccg caccgattga tgccgacggt aacgtgctgg cgctgcgccc ggagtttgcc
    14341 gaaaacccaa acgcgatgtt cgtattgtgg aaagaccaca ctgcggttga agaagcggaa
    14401 gagattaccc gtttgtgcca cgcgccgggc aacgttgact actcccgcta cattggtggt
    14461 atttattcca gcgaatggtt ctgggcaaaa atcctgcatg tgactcgcca ggacagcgcc
    14521 gtggcgcaat ctgccgcatc gtggattgag ctgtgcgact gggtgccagc tctgctttcc
    14581 ggtaccaccc acccgcagga tattcgtcgc ggacgttgca gcgccgggca taaatctctg
    14641 tggcacgaaa gctggggcgg cttgccgcca gccagtttct ttgatgagct ggacccgatc
    14701 ctcaatcgcc atttgccttc cccgctgttc actgacacct ggactgccga tattccggtg
    14761 ggcaccttat gcccggaatg ggcgcagcgt ctcggcctgc ctgaaagcgt ggtgatttcc
    14821 ggcggcgcgt ttgactgcca tatgggcgca gttggcgcag gcgcacagcc taacgcactg
    14881 gtaaaagtta tcggtacttc cacctgcgac attctgattg ccgacaaaca gagcgttggc
    14941 gagcgggcag ttaaaggtat ttgcggtcag gttgatggca gcgtggtgcc tggatttatc
    15001 ggtctggaag caggccaatc ggcgtttggt gatatctacg cctggttcgg tcgcgtactc
    15061 agctggccgc tggaacagct tgccgcccag catccggaac tgaaagcgca aatcaacgcc
    15121 agccagaaac aactgcttcc ggcgctgacc gaagcatggg ccaaaaatcc gtctctggat
    15181 cacctgccgg tggtgctcga ctggtttaac ggtcgtcgca caccgaacgc taaccaacgc
    15241 ctgaaagggg tgattaccga tcttaacctc gctaccgacg ctccgctgct gttcggcggt
    15301 ttgattgctg ccaccgcctt tggcgcacgc gcaatcatgg agtgctttac cgatcagggg
    15361 atcgccgtca ataacgtgat ggcgctgggc ggcatcgcgc ggaaaaacca ggtcattatg
    15421 caggcctgct gcgacgtgct gaatcgcccg ctgcaaattg ttgcctctga ccagtgctgt
    15481 gcgctcggtg cggcgatttt tgctgccgtc gccgcgaaag tgcacgcaga catcccatca
    15541 gcccagcaaa aaatggccag tgcggtagag aaaaccctgc aaccgcgcag cgaacaggca
    15601 caacgctttg aacagcttta tcgccgctat cagcaatggg cgatgagcgc cgaacaacac
    15661 tatcttccaa cttccgcccc ggcacaggct gcccaggccg ttgcgactct ataaggacac
    15721 gataatgacg atttttgata attatgaagt gtggtttgtc attggcagcc agcatctgta
    15781 tggcccggaa accctgcgtc aggtcaccca acatgccgag cacgttgtta atgcgctgaa
    15841 tacggaagcg aaactgccct gcaaactggt gttgaaaccg ctgggcacca cgccggatga
    15901 aatcaccgct atttgccgcg acgcgaatta cgacgatcgt tgcgctggtc tggtggtgtg
    15961 gctgcacact ttctccccgg ccaaaatgtg gatcaacggc ctgaccatgc tcaacaaacc
    16021 gttgctgcaa ttccacaccc agtttaacgc ggcgctgccg tgggacagta tcgatatgga
    16081 ctttatgaac ctgaaccaga ctgcacatgg cggtcgcgag ttcggcttca ttggcgcgcg
    16141 tatgcgtcag caacatgccg tggttaccgg tcactggcag gataaacaag cccatgagcg
    16201 tatcggctcc tggatgcgcc aggcggtctc taaacaggat acccgtcatc tgaaagtctg
    16261 ccgttttggc gataacatgc gtgaagtagc ggtcaccgat ggtgataaag ttgccgcaca
    16321 gatcaagttc ggtttctccg tcaatacctg ggcggttggc gatctggtgc aggtggtgaa
    16381 ctccatcagc gacggcgatg ttaacgcgct ggtcgatgag tacgaaagct gctacaccat
    16441 gacgcctgcc acacaaatcc acggcgaaaa acgacagaac gtgctggaag cggcgcgtat
    16501 tgagctgggg atgaagcgtt tcctggaaca aggtggcttc cacgcgttca ccaccacctt
    16561 tgaagatttg cacggtctga aacagcttcc aggtctggcc gtacagcgtc tgatgcagca
    16621 gggttacggc tttgcgggcg aaggcgactg gaaaaccgcc gccctgcttc gcatcatgaa
    16681 ggtgatgtca accggtctgc agggcggcac ctcctttatg gaggactaca cctatcactt
    16741 cgagaaaggt aatgacctgg tgctcggctc ccatatgctg gaagtctgcc cgtcgattgc
    16801 cgtagaagag aaaccgatcc tcgacgttca gcatctcggt attggtggta aggacgatcc
    16861 tgcccgactg atcttcaata cccaaaccgg tccagcgatt gtcgccagcc tgattgatct
    16921 cggcgatcgt taccgtctgc tggttaactg tatcgacacg gtgaaaacac cgcactccct
    16981 gccgaaactg ccggtggcga atgcgctgtg gaaagcgcaa ccggatctgc caactgcttc
    17041 cgaagcgtgg atccttgctg gtggcgcgca ccataccgtc ttcagccatg cgctgaacct
    17101 caacgatatg cgccagttcg ccgagatgca cgacattgaa atcacggtga ttgataacga
    17161 cacccgcctg ccagcgttta aagacgcact gcgctggaac gaagtgtatt acggatttcg
    17221 tcgctaagta gccacatcag gtatgtaacg cctgatgcga cgctgacgcg tcttatctgg
    17281 cctacacgct gcgattttgt aggccggata agcaaagcgc atccggcatt gttttccccc
    17341 tcgccccttt ggggagaggg ccggggtgag gggaaacgac ctctcaggag tcaacatgtt
    17401 agaagatctc aaacgccagg tattagaagc caacctggcg ctgccaaaac acaacctggt
    17461 cacgctcaca tggggcaacg tcagcgccgt tgatcgcgag cgcggcgtct ttgtgatcaa
    17521 accttccggc gtcgattaca gcgtcatgac cgctgacgat atggtcgtgg ttagcatcga
    17581 aaccggtgaa gtggttgaag gtacgaaaaa gccctcctcc gacacgccaa ctcaccggct
    17641 gctctatcag gcattcccct ccattggcgg cattgtgcat acgcactcgc gccacgccac
    17701 catctgggcg cagtcgggtc agtcgattcc agcaatcggc accacccacg ccgactattt
    17761 ctacggcacc attccctgca cccgcaaaat gaccgacgca gaaatcaacg gcgaatatga
    17821 gtgggaaacc ggtaacgtca tcgtagaaac ctttgaaaaa cagggtatcg atgcagcgca
    17881 aatgcccggc gttctggtcc attcccacgg cccgtttgca tggggcaaaa atgccgaaga
    17941 tgcggtgcat aacgccatcg tgctggaaga ggtcgcttat atggggatat tctgccgtca
    18001 gttagcgccg cagttaccgg atatgcagca aacgctgctg gataaacact atctgcgtaa
    18061 gcatggcgcg aaggcatatt acgggcagta atgactgtat aaaaccacag ccaatcaaac
    18121 gaaaccaggc tatactcaag cctggttttt tgatggattt tcagcgtggc gcaggcaggt
    18181 tttatcttaa cccgacactg gcgggacacc ccgcaaggga cagaagtctc cttctggctg
    18241 gcgacggaca acgggccgtt gcaggttacg cttgcaccgc aagagtccgt ggcgtttatt
    18301 cccgccgatc aggttccccg cgctcagcat attttgcagg gtgaacaagg ctttcgcctg
    18361 acaccgctgg cgttaaagga ttttcaccgc cagccggtgt atggccttta ctgtcgcgcc
    18421 catcgccaat tgatgaatta cgaaaagcgc ctgcgtgaag gtggcgttac cgtctacgag
    18481 gccgatgtgc gcccgccaga acgctatctg atggagcggt ttatcacctc accggtgtgg
    18541 gtcgagggtg atatgcacaa tggcactatc gttaatgccc gtctgaaacc gcatcccgac
    18601 tatcgtccgc cgctcaagtg ggtttctata gatattgaaa ccacccgcca cggtgagctg
    18661 tactgcatcg gcctggaagg ctgcgggcag cgcatcgttt atatgctggg gccggagaat
    18721 ggcgacgcct cctcgcttga tttcgaactg gaatacgtcg ccagccgccc gctgctgctg
    18781 gaaaaactca acgcctggtt tgcaaaccac gatcccgatg tgatcatcgg ctggaacgtg
    18841 gtgcagttcg atctgcgaat gctgcaaaaa catgccgagc gttaccgtct tccgctgcgt
    18901 cttgggcgcg ataatagcga gctggagtgg cgcgagcacg gctttaaaaa cggcgtcttt
    18961 tttgcccagg ctaaaggtcg gctaattatc gacggtatcg aggcgctgaa atccgcgttc
    19021 tggaatttct cttcattctc gctggaaact gtcgctcagg agctattagg cgaaggaaaa
    19081 tctatcgata acccgtggga tcgaatggac gaaattgacc gccgtttcgc cgaagataaa
    19141 cctgcgctgg caacttataa cctgaaagat tgcgagctgg tgacgcagat cttccacaaa
    19201 actgaaatca tgccattttt actcgaacga gcaacggtga acggcctgcc ggtggatcgg
    19261 cacggcggtt cggtggcagc gtttggtcat ctctattttc cgcgaatgca tcgcgctggt
    19321 tatgtcgcgc ctaatctcgg cgaagtgcca ccgcacgcca gccctggcgg ctacgtgatg
    19381 gattcacggc cagggcttta tgattcagtg ctggtgctgg actataaaag cctgtacccg
    19441 tcgatcatcc gcacctttct gattgatccc gtcgggctgg ttgaaggcat ggcgcaacct
    19501 gatccagagc acagtaccga aggttttctc gatgcctggt tctcacggga gaaacattgc
    19561 ctgccggaga ttgtgactaa catctggcac gggcgcgatg aagccaaacg tcagggtaat
    19621 aagccgcttt cgcaggcgct gaagatcatt atgaatgcct tttatggcgt gctcggcacc
    19681 accgcctgcc gcttcttcga tccgcgcctg gcatcgtcga tcaccatgcg tggtcatcag
    19741 atcatgcggc agaccaaagc attgattgaa gcgcagggct acgacgttat ctacggagat
    19801 accgactcga cttttgtctg gctaaaaggt gcacattcgg aagaagaggc ggcgaaaatc
    19861 ggtcgtgtac tggtgcagca cgttaacgcc tggtgggcgg aaacgctgca aaaacaacgg
    19921 ctgaccagcg cattagaact ggagtatgaa actcatttct gccgttttct gatgccaacc
    19981 attcgcggag ccgataccgg cagtaaaaag cgctacgccg ggctgattca ggagggcgat
    20041 aaacagcgga tggtgtttaa agggctggaa accgtgcgca ccgactggac gccgctggcc
    20101 cagcagtttc agcaggagct atacctgcgc atcttccgca acgagccata tcaggaatat
    20161 gtacgcgaaa ccatcgacaa actgatggcg ggtgaactgg atgcgcgact ggtttaccgt
    20221 aaacgccttc gccgtccgct gagcgagtat cagcgtaatg tgccgcctca tgtacgcgcc
    20281 gctcgccttg ccgatgaaga aaaccaaaag cgcggtcgcc ccttgcaata tcagaatcgc
    20341 ggcaccatta agtacgtatg gaccaccaac ggcccggagc cgctggacta ccaacgttca
    20401 ccactggatt acgaacacta tctgacccgc cagctacaac ccgtggcgga gggaatactc
    20461 ccttttattg aggataattt tgctacactt atgaccgggc aacttgggct attttgagca
    20521 aaaaaaagag ttcgccagat accattttga tgcgtgacga atgctttgcc atccagtacc
    20581 atagcgccct ttccattcct ggacctgaat aacaccacta cctcataagc acggtagcgg
    20641 gtggttattg cctgcaatta aagatataga gccgaacaca tatgcctttt acacttggtc
    20701 aacgctggat cagcgataca gaaagcgaat tgggacttgg aaccgttgtc gcggtggatg
    20761 cgcgaactgt cactttactt ttcccatcta ctggtgaaaa ccgtctgtac gcacgcagtg
    20821 attcccccgt gacccgcgtg atgttcaacc ctggtgatac tattaccagc catgacggct
    20881 ggcagatgca agtcgaagaa gtaaaagaag aaaatggctt gctgacctat atcggtactc
    20941 gcctggatac tgaagagtcc ggcgtagccc tgcgtgaagt tttccttgat agcaaactgg
    21001 tgttcagcaa accgcaggac cgtctgtttg ccgggcagat tgaccgtatg gaccgctttg
    21061 cgctgcgtta tcgcgcgcgt aagtattcca gcgaacagtt ccgtatgccg tacagcggcc
    21121 tgcgcggtca gcgtaccagc ctgattccgc atcagctcaa catcgctcat gatgttggtc
    21181 gccgccacgc gccgcgcgtc ctgctggctg acgaagtggg tttagggaaa accattgaag
    21241 ccgggatgat cctgcatcag caactgctct ctggcgctgc tgaacgtgtg ctaattatcg
    21301 tcccggaaac cttacagcat cagtggctgg tagaaatgct gcgccgtttc aacctgcgct
    21361 ttgcgctatt tgatgatgag cgttatgccg aagctcagca cgatgcttac aacccgtttg
    21421 acaccgaaca gctggtgatt tgctcgctgg attttgcccg tcgtagcaaa cagcgcctgg
    21481 aacatctctg tgaagccgaa tgggacctgc tggtggtcga tgaagcgcat cacctggtgt
    21541 ggagcgaaga tgcgccaagc cgtgaatatc aggccattga acaactggca gagcacgtgc
    21601 cgggcgttct gctgctgacc gcgaccccgg aacagctggg gatggaaagc cacttcgccc
    21661 gtctgcgtct gctggacccg aaccgtttcc acgattttgc gcagttcgtt gaagagcaga
    21721 aaaattatcg tccggttgcg gacgccgttg ccatgctgct ggcaggtaac aaactgagca
    21781 atgacgaact gaacatgctc ggcgagatga tcggcgagca ggatatcgag ccgctgttgc
    21841 aggcagcaaa cagcgacagc gaagatgccc agagcgcccg tcaggagctg gtttcgatgc
    21901 tgatggatcg ccacggcacc agccgcgtgc tgttccgtaa cacgcgtaac ggtgtgaaag
    21961 gattcccgaa acgcgagctg cacaccatta aactgccgct accgacgcag tatcagacgg
    22021 ctattaaagt ctccggcatt atgggcgcac gtaaaagtgc ggaagatcgt gctcgcgata
    22081 tgctctaccc ggagcgtatt tatcaggaat ttgaaggtga taacgccacc tggtggaact
    22141 tcgatccgcg cgttgagtgg ctgatgggct acctgaccag ccatcgctct cagaaagtgc
    22201 tggtgatctg cgccaaagct gccactgcgc tgcaactgga gcaggtactg cgcgaacgtg
    22261 aaggtattcg cgctgcggtg ttccacgaag gtatgtcgat tatcgaacgt gaccgcgctg
    22321 ccgcctggtt tgccgaagaa gacaccggcg cacaggtact gctgtgctca gaaatcggtt
    22381 ctgaaggacg taacttccag ttcgccagcc acatggtgat gtttgacctg ccattcaacc
    22441 cggatctact ggagcagcgt attggtcgtc tggatcgtat cggccaggcg cacgatattc
    22501 agatccatgt gccttatctg gagaaaaccg ctcagtcggt gctggtgcgc tggtatcacg
    22561 aaggtctgga tgcatttgag cacacctgcc cgaccggacg cactatttac gatagcgtat
    22621 acaacgatct gattaactat ctggcttcac cggatcaaac cgaaggcttt gacgatctga
    22681 tcaaaaactg ccgcgagcaa catgaagcgc tgaaagcaca gctggaacag ggtcgtgacc
    22741 gcctgctgga aatccactcc aacggtggcg aaaaagccca ggcactggca gaaagcattg
    22801 aagagcagga tgacgatacc aacctgatcg ccttcgccat gaacctgttc gatattatcg
    22861 gtatcaatca ggacgatcgc ggcgacaaca tgatcgtgct gacgccgtcc gatcatatgc
    22921 tggtgccgga cttccctggc ctgtcggaag atggcatcac catcaccttt gatcgtgaag
    22981 tggcgctggc gcgtgaagat gcacagttta ttacctggga gcatccgctg atccgcaacg
    23041 gtctggatct gattctttct ggcgataccg gtagcagcac gatttcactg ttaaaaaaca
    23101 aagcgttgcc ggtaggtacg ctgttggtgg aactgattta tgtggttgaa gcccaggctc
    23161 cgaagcagtt gcagctcaac cgcttcctgc caccgacgcc ggtacgtatg ctgctggata
    23221 aaaacggcaa caacctggcg gcgcaggtag agtttgaaac ctttaaccgc cagcttaacg
    23281 cggttaaccg tcacaccggc agcaaactgg ttaacgccgt gcagcaggat gttcacgcta
    23341 tccttcaact gggtgaagcg cagatcgaga aatctgcccg tgcattgatt gatgcagcgc
    23401 gtaacgaagc cgacgaaaaa ctgtctgccg agctgtctcg tctggaagct ctgcgtgcag
    23461 tgaacccgaa cattcgtgac gacgaactga ccgccattga gagcaaccgt cagcaggtaa
    23521 tggaaagcct ggatcaggca ggttggcgtc tggatgccct gcgtttgatc gttgtaacgc
    23581 atcagtaacg gagccgaaaa tggggatgga aaactacaat ccaccgcagg aaccctggtt
    23641 ggttatcctg tatcaggatg accatattat ggtggtcaac aagccgagcg gtttgttgtc
    23701 agtgccgggt cgtctggaag agcacaaaga cagcgtgatg acgcgcattc agcgtgatta
    23761 tccgcaggca gaatcggtgc atcgtctgga tatggctacc agcggcgtga ttgtagtggc
    23821 gctgaccaaa gccgcggagc gggagttaaa acgccagttc cgcgagcgcg agccgaaaaa
    23881 gcagtatgtg gcccgcgtct gggggcatcc atcccccgca gaaggtctgg tggatctgcc
    23941 gctgatttgc gactggccaa accgcccgaa acagaaagtc tgttacgaaa cgggtaaacc
    24001 tgcgcagacg gaatatgaag tggtggagta tgcggcggat aacacggcaa gagtggtgtt
    24061 aaaaccgatt accgggcgtt cgcatcaatt gcgtgtgcat atgctggcgc tggggcatcc
    24121 gattctcggc gatcgttttt atgcatcacc agaagcgaga gcgatggcac cgcgtttgtt
    24181 gctacatgca gagatgttga cgattaccca tccggcgtat ggcaatagta tgacgtttaa
    24241 agcgccagcg gatttttaat cgtcacgctg gcgcagttcg gtaggcggat aaggcgttca
    24301 cgccgcatcc ggcaattgtg cgccgattgc ctgatgcgac gcgggccgcg tcttatcagg
    24361 cctacagttt aaaacttatt taaacccttt ctgctgcttt atcagctcat atgcctgctg
    24421 aatttcctgc gctttctgct tcgccatctc catcatctca ggcggcaaac ctttcgccac
    24481 cagcttatcg ggatggtgtt cactcatcag cttacggtag gcacgtttga tggtggtcgc
    24541 atcatccgtc ggcttcacgc ccagcacatt acaggcatct tccagcgttg ggccacgctg
    24601 cgcttgctgc cagttaccac cgccagtttg ctgctgataa ccgccgccaa actgtgcacc
    24661 gccctgcatc atgcgcaaaa actggtcaaa ctgggcgcgg gagatcccta attcttcagc
    24721 aatgacatac agcaccgccc gttcattcgg gtgcagtgaa ccatcagcaa acgccgcctg
    24781 aatctggatc tccagaaaca tacgaattaa gtcaaaacga ccaaagcaga cactgcgaaa
    24841 ctggcgcatc ttttcgcgca gcgggtaatt gtctgatttt cccacccgga acgcattttg
    24901 cgccgcagta cgggaagcgc catgaagatt cattcggtcc atcaactggc tggcgatatg
    24961 aatatcagcc tccgtgacgc gacctttgga tttggttaaa tgccccatca cttcaaaagt
    25021 ggtggcaaaa aacagcgcct gacgctcacg ctggttggcg aaccacgcca ttttacggct
    25081 acgggcttta tcaaacatat ggccaattaa caggcctaac actacgcccc aaaagccgcc
    25141 gcccatcagt aaggccacgg ccacgccaat gatttttccc caatactgca tatattcccc
    25201 aaatcgacac acggatatca gggctatctc ccacaatata aaggtgcttt taccgttttc
    25261 cggctgcggt caattgtggg acatcgccta taatttgcat tatcatacct gtcattcaat
    25321 gccgtgccta acaccactga cgctattcgg acaggattaa cactagcgta gagatgacga
    25381 gtacgttagt ctctgaccgt ttgtcacgcg caacgttacc gatgatggaa caataaaatc
    25441 aacgtatgaa aaaacgtatc cccactctcc tggccaccat gattgccacc gccctttata
    25501 gtcaacaggg actggcagcc gacctcgcct cacagtgcat gttgggcgtg ccaagctatg
    25561 accgtcctct ggtacagggc gataccaatg acttacccgt gactatcaat gctgaccacg
    25621 cgaaagggga ctacccggat gacgccgtgt ttactggcag cgtggatatc atgcagggta
    25681 acagccgtct gcaggccgac gaagtgcagc tccatcaaaa agaggcacca ggacaaccgg
    25741 agccggtacg taccgttgat gcgctcggta atgtccatta cgacgataac caggtgatcc
    25801 tcaaagggcc gaaaggctgg gcgaatctga acaccaaaga taccaacgtc tgggaaggtg
    25861 attaccagat ggtgggtcgc cagggtcgcg gtaaagcgga cctgatgaaa caacgtggcg
    25921 aaaaccgcta taccattctg gataacggta gctttacctc ctgtctgccg ggttctgaca
    25981 cctggagcgt ggtaggtagc gaaattattc atgaccgcga agaacaagtt gcggagatct
    26041 ggaacgcccg ctttaaggtg ggtccggtac cgatctttta tagcccctat ttgcagttgc
    26101 cggtgggtga caaacgtcgc tctggtttct tgatcccgaa cgccaagtac accaccacca
    26161 actactttga gttctacctg ccatattact ggaacatcgc gccaaatatg gatgccacca
    26221 tcacgccgca ttatatgcat cgtcgtggca acatcatgtg ggagaacgaa ttccgctacc
    26281 tctcccaggc gggcgctggc ttgatggaac tggactatct gccttcagat aaagtctatg
    26341 aagatgaaca cccgaacgat gacagttcac gtcgttggtt attctactgg aaccactccg
    26401 gggtcatgga tcaggtgtgg cgtttcaacg tcgactacac caaggtcagc gatcctagct
    26461 acttcaatga tttcgataac aagtacggtt ccagtactga cggctacgca acgcaaaaat
    26521 tcagcgttgg ctatgcggtg caaaacttca atgccaccgt ttcaaccaag cagttccagg
    26581 ttttcagcga acagaacacc agtagctact cggcagagcc gcagttagac gttaattact
    26641 accagaatga tgttggtccg tttgatacgc gtatttacgg ccaggcagtg cactttgtta
    26701 acaccagaga cgacatgcct gaagcaaccc gtgttcacct ggaaccgacc atcaatttgc
    26761 cgctctctaa taactggggc agcatcaata ccgaagcgaa gttgctggca acccattatc
    26821 agcaaactaa tcttgactgg tataactcca gaaacacgac caagctggac gaatccgtta
    26881 accgcgtaat gccgcaattc aaagttgacg gcaaaatggt ctttgaacgc gatatggaaa
    26941 tgctggctcc gggttatacc caaacgctgg aaccgcgcgc gcagtatttg tacgtgccgt
    27001 atcgcgatca gagcgacatc tataactacg actcatctct gctgcaatct gactactctg
    27061 gcctgttccg ggaccggact tacggcggtc ttgaccgtat tgcctccgct aaccaggtga
    27121 cgaccggtgt cacatctcgc atatatgatg atgctgccgt tgaacgtttt aatatttccg
    27181 ttggtcaaat ctactatttc acggagtctc gcactggcga tgacaacata acatgggaga
    27241 atgacgacaa aacgggctca ctggtgtggg caggcgatac ttactggcgt atctccgagc
    27301 gttggggatt gcgtggcggg attcagtacg atacacgtct ggataacgta gcgaccagta
    27361 actccagcat tgaataccgt cgggatgaag accgtctggt acagctgaat taccgttacg
    27421 ccagcccgga atatattcag gctacgctgc ctaagtacta ttccactgct gagcaatata
    27481 agaatggtat ttcgcaggta ggtgctgtcg ccagctggcc aattgccgat cgttggtcca
    27541 ttgttggggc ctactactac gacaccaatg cgaacaagca agccgactct atgttaggtg
    27601 tgcaatacag ctcctgctgc tatgcaattc gcgtcggtta cgagcggaag ctgaacggtt
    27661 gggataacga taaacaacat gcggtatatg acaacgcaat cggctttaac atcgaacttc
    27721 gcggcctgag ctccaactac ggtctgggta cgcaagagat gctgcgttcg aacattctgc
    27781 cgtatcaaaa cactttgtga tttgttgatt taccacgtaa tccgcagtgc ggttaattga
    27841 aatggaaaaa gtatgaagaa ctggaaaacg ctgcttctcg gtatcgccat gatcgcgaat
    27901 accagtttcg ctgcccccca ggtagtcgat aaagtcgcag ccgtcgtcaa taacggcgtc
    27961 gtgctggaaa gcgacgttga tggattaatg cagtcggtaa aactgaacgc tgctcaggca
    28021 aggcagcaac ttcctgatga cgcgacgctg cgccaccaaa tcatggaacg tttgatcatg
    28081 gatcaaatca tcctgcagat ggggcagaaa atgggagtga aaatctccga tgagcagctg
    28141 gatcaggcga ttgctaacat cgcgaaacag aacaacatga cgctggatca gatgcgcagc
    28201 cgtctggctt acgatggact gaactacaac acctatcgta accagatccg caaagagatg
    28261 attatctctg aagtgcgtaa caacgaggtg cgtcgtcgca tcaccatcct gccgcaggaa
    28321 gtcgaatccc tggcgcagca ggtgggtaac caaaacgacg ccagcactga gctgaacctg
    28381 agccacatcc tgatcccgct gccggaaaac ccgacctctg atcaggtgaa cgaagcggaa
    28441 agccaggcgc gcgccattgt cgatcaggcg cgtaacggcg ctgatttcgg taagctggcg
    28501 attgctcatt ctgccgacca gcaggcgctg aacggcggcc agatgggctg gggccgtatt
    28561 caggagttac ccgggatctt cgcccaggca ttaagcaccg cgaagaaagg cgacattgtt
    28621 ggcccgattc gttccggcgt tggcttccat attctgaaag ttaacgacct gcgcggcgaa
    28681 agcaaaaata tctcggtgac cgaagttcat gctcgccata ttctgctgaa accgtcgccg
    28741 atcatgactg acgaacaggc ccgtgtgaaa ctggaacaga ttgctgctga tatcaagagt
    28801 ggtaaaacga cttttgctgc cgcagcgaaa gagttctcgc aggatccagg ttctgctaac
    28861 cagggcggcg atctcggttg ggcaacagcg gacatttttg atccggcttt ccgtgacgcc
    28921 ctgacccgcc tgaacaaagg tcaaatgagt gcaccggttc actcttcatt cggctggcat
    28981 ttaatcgaac tgctggatac ccgtaatgtc gataaaaccg acgctgcgca gaaagatcgt
    29041 gcataccgca tgctgatgaa ccgtaagttc tcggaagaag cagcaagctg gatgcaggaa
    29101 caacgtgcca gcgcctacgt taaaatcctg agcaactaat ggttaaaacc caacgtgttg
    29161 tgatcactcc cggcgagccc gccgggattg gcccggactt agttgtccag cttgcacagc
    29221 gtgagtggcc ggtcgaactg gttgtttgtg ccgatgccac tctccttacc gaccgggcag
    29281 cgatgctcgg tttgccgctc accctccgca cttattcccc caactctcct gcacaaccgc
    29341 aaactgcggg cacattaacg ctacttcctg tcgcgctacg tgaatctgtc actgcggggc
    29401 agttagcggt tgaaaatggg cattatgtgg tggaaacgct ggcgcgagcg tgcgatggct
    29461 gtctgaacgg tgaatttgct gcgctgatca caggccccgt gcataaaggc gtcattaacg
    29521 acgcaggcat tccgtttacc ggtcataccg agtttttcga agagcgttcg caggcgaaaa
    29581 aagtggtgat gatgctggcg accgaagaac ttcgcgtggc gctggcaacg acgcatttac
    29641 cgctgcgcga tatcgcagat gctatcaccc ctgcgctttt gcacgaagtg attgctattt
    29701 tgcatcacga tttgcggacc aaatttggta ttgccgaacc gcgcattctg gtctgcgggc
    29761 tgaatccaca cgcgggcgaa ggcggtcata tgggtacgga agagatagac accattattc
    29821 cgctgctcga cgagttgcgg gcgcagggga tgaaacttaa cgggccgctg cctgccgata
    29881 ccctgtttca gccgaaatat ctcgataacg ccgacgccgt gctggcgatg taccacgatc
    29941 agggtcttcc cgtgctaaaa taccagggct tcgggcgcgg tgtgaacatt acgctgggcc
    30001 tgccctttat tcgcacatca gtggaccacg gcaccgcgct tgaactggcg ggacgtggcg
    30061 aagccgatgt cggcagtttt attacggcgc ttaatctcgc catcaaaatg attgttaaca
    30121 cccaatgaat aatcgagtcc accagggcca cttagcccgt aaacgcttcg ggcaaaactt
    30181 tctcaacgat cagttcgtga tcgacagcat tgtttctgcc attaacccgc aaaagggcca
    30241 ggcaatggtc gaaatcggcc ccggtctggc ggcattgacc gagccggtcg gcgaacgtct
    30301 ggaccagctg acggtcatcg aacttgaccg cgatctggcg gcacgtctgc aaacgcatcc
    30361 attcttaggc ccgaaactga cgatttatca gcaggatgcg atgaccttta actttggcga
    30421 actggccgag aaaatgggtc agccgctgcg tgttttcggc aacctgcctt ataacatctc
    30481 cacgccgttg atgttccatc tgtttagcta tactgatgcc attgccgaca tgcactttat
    30541 gttgcaaaaa gaggtggtga atcgtctggt tgcaggaccg aacagcaaag cgtatggtcg
    30601 attaagcgtc atggcgcaat actattgcaa cgtgatccca gtgctggaag taccgccgtc
    30661 agcctttaca ccaccaccca aagtggattc cgccgtcgtg cgcctggttc ctcatgcaac
    30721 gatgcctcac ccggttaaag atgttcgtgt gttgagccgc atcaccaccg aagcctttaa
    30781 ccagcgtcgt aaaaccattc gtaacagcct cggcaacctg tttagcgtcg aggtgttaac
    30841 gggaatgggg atcgacccgg cgatgcgagc ggaaaatatc tctgtcgcgc aatattgcca
    30901 gatggcgaac tatctggcgg agaacgcgcc tttgcaggag agttaacgat gatcaattcg
    30961 ccccgagtgt gtattcaggt tcaaagcgtc tacattgagg cacaatcttc acctgataat
    31021 gaacgttacg tttttgctta taccgtaacc atacgcaatc tggggcgagc gccagtgcag
    31081 ttgttggggc gttactggct gatcaccaat ggcaatggcc gtgaaaccga agtccagggc
    31141 gaaggagtgg ttggcgtcca gccacttatc gcgcctggcg aagagtacca gtacaccagc
    31201 ggtgcaatca ttgaaacccc gctgggcacc atgcagggtc actacgaaat gatcgatgaa
    31261 aatggcgtcc ctttcagcat cgacattccc gtattccgac tcgccgttcc cacactcatt
    31321 cattaaaaga atatggcgac ataccttatt ggcgacgttc atggttgtta cgatgaactg
    31381 atcgcattgc tgcataaagt agaatttacc cctgggaaag ataccctctg gctgacgggc
    31441 gatctggtcg cgcgcgggcc gggttcgctg gatgttctgc gctatgtgaa atccttaggc
    31501 gacagcgtac gtctggtgct gggcaatcac gatctgcatc tgctggcggt atttgccggg
    31561 atcagccgca ataaaccgaa agatcgcctg acaccgctgc tggaagcgcc ggatgccgac
    31621 gagctgctta actggctgcg tcgccagcct ttgctgcaaa tcgacgaaga gaaaaagttg
    31681 gtgatggccc acgcagggat cacgccgcag tgggatctac agaccgccaa agagtgcgcg
    31741 cgcgatgtag aagcggtgct atcgagtgac tcctatccct tctttctcga cgctatgtac
    31801 ggcgatatgc caaataactg gtcaccggaa ttgcgggggc tgggaagact gcgttttatc
    31861 accaacgcct ttacccgtat gcgtttttgc tttccaaacg gtcaactgga tatgtacagc
    31921 aaagaatcgc cggaagaagc ccctgcccca ctgaaaccgt ggtttgcgat tcctggccct
    31981 gtcgctgaag aatacagcat cgcctttggt cactgggcat cgctggaagg caaaggtacg
    32041 ccggaaggta tttacgcgct ggataccggc tgctgctggg gtgggtcatt aacctgcctg
    32101 cgctgggaag ataagcagta ttttgtccag ccgtcgaacc ggcataagga tttgggcgaa
    32161 gcggcggcgt cttaaacaca gcctgatata ggaaggccgg ataagacgcg accggcgtcg
    32221 catccggcac tagccgtaaa ttctatacaa aattaccgcc gctccagaat ctcaaagcaa
    32281 tagctgtgag agttctgcgc atcggcgtcg tggaattcgc tgaataccga ttcccagtca
    32341 tccggctcgt aatccgggaa atgggtgtcg ccttccactt ctgcgtcgat atgcgtcaga
    32401 tacagttttt gcgcttttgg caggaactgc tcataaacgc gaccgccgcc aatcaccatg
    32461 atttctggta cgtcaccaca cgccgcgatg gcttcatcca ccgacttcac ccacgttacg
    32521 cgatcgtccg tacccggttg actgctgagg ataatatttt tgcgtcctgg caacggacga
    32581 ccgattgatt cccaggtatg gcggcccata atcacgggtt tatttaaggt gttgcgttta
    32641 aaccaggcga gatcggcagg caggttccac ggcatggcgt tttccatgcc gataacgcga
    32701 tctaccgcta acgccgcaat cagactgatc attgagattt cccgataaaa aaaattgtcg
    32761 ccactatacg taaagcgtaa accgtcgtcg actggtgcga ggatgatgtt gaggaaaatt
    32821 ttatattctg ctggcgagtc cacgctctct ccctggactc gccgcattac aatgaaacaa
    32881 aaacaaacag ttagctgtaa agtgtgattt acgtcactct ttattaggat gagggtttcg
    32941 tttccggttc atccgccatg ttgccggtat gtttaccttc ttcggttccc tgccagccat
    33001 gtcgttgaat taatgacaga tgttcgcggt cctcggtaat gatctcactt aacatcgcgc
    33061 tggtgcgttt atagaccgcc gcgcgggctt tggtatcatt ctcaaccatt gccatctctt
    33121 ccaccatctg aatattaaag cggcggaaca catcggcacg ttctcgcgct tcatacggcc
    33181 ccagacctaa actttccagt gccagacgcc cggttttcag cgcaccttcg aaggtttcac
    33241 gctccggctt ttcaacgcct gcctgacgca aacgaatgta gtggtcgaca tcgcgggcgc
    33301 gggcaataat ctgcaaatgc gggaaatgtt ctttcaccat ctctgtcagt tgcaggttgg
    33361 tttgcggatc gtcgatggcg ttaatcagca cttccgcttt cgccgctccg gcagattcca
    33421 gtaaatccat ccgcgtggca tcgccataaa acactttcat accaaattta cgcaaggttt
    33481 cgatatggtc cggatcgtga tcgagtacca ccattttcac cccgctggag agcagtaaac
    33541 gtccggtaat ctgcccaaaa cgaccgaatc cggcgataat cacgcgcggc tgttcttcgt
    33601 cgatctcatc ggcttcacgc gcttcctcag tagaagattg ctcaaggcga ttgaggatca
    33661 ccagcagaat cggcgttgct gccatcgaca gcgccaccgc cagggtcagc gatttcgccc
    33721 actccggctc cagcacattc gccatctgcg ccgcgccaaa taccacaaag gcaaactcac
    33781 tgccctgccc taacaacacc gcaaaccaac gacgctgttt atttggcact tgcaacggtc
    33841 gggcaatcag ccacagcatg gcgattttga tgatgaggaa accgagcagc aaaatgacaa
    33901 tgcgcaatgg gttttcaagc agcgtgccaa agtctatcga catgccaaca ccgatgaaaa
    33961 acagccccaa cagcaaacct ttaaatggtt cgatatcgct ctccagcgca tgacggtatt
    34021 ccgagcttgc cagcagtacg cccgccagaa acgcgcccat cgccatcgac aagccgacct
    34081 cttccagcag caaaccaaag ccaaacacga ggaataacgc cacggcacta aacacttccc
    34141 gcaagccaga gcgggctaca aaacgcagcg ccggacgcgt gacatagcgc cccagcaata
    34201 ccaccagcac cagcgcaccc gccactttta acgccgagag agcaaatgcg cccatcgtcg
    34261 tcgaggcact gctcgttgcc agtagcggaa tcatcgccac cagcgggatc gccgcgatat
    34321 cctggaacag cagcaccgca aaggcactgc gacccatttg cgtcaccatc agattgcgtt
    34381 cattcatcgc ctgcatggca atcgccgtag aggagagcgc cagcgtcatg ccgatcaatt
    34441 ccgcgacctg ccagcgcagc ccaagtaaca tgcagaacag ccccagcagg ccgccgcaaa
    34501 tcaccatctg caatgcgcca ccgccgaaca ctgccgcacg cagcttccac agcctttgtg
    34561 gatcgagttc gaggccgata ataaacagca tcagcaccac gccaatctcg gcaaagtgca
    34621 gaatagattc ggcatcggtc accagtcgca gcccccacgg gccaataatg cagccggcga
    34681 tcaggtagcc aagtaccgat cccagaccaa gacgtaccgc aatgggtaca atcagcgctg
    34741 ccgaaccgag ataaatcagc gcctgaatca gcgtatggct atccatgatg ggcctcctgc
    34801 cattccagca gacgttgctt atagtgacgc gcctgccctt cgagggtttc gtcgtcacaa
    34861 ataaaggtgc agtgcatggc aaacggtggc agccagttca gcccgcagta gattgccgtc
    34921 gcctgtagcg gctgcgacag cacatcaaag cccggatgcg caccaatttc aaaatggctt
    34981 tccccgccgc cggtcgtcac cgcccacagc aaatgtttgc catgcagcgc cgtgccgccg
    35041 tgaccgtaag cccagccgtg cgagaaaact ttatcgatcc aaagtttgag gagcggtgga
    35101 atgctgtacc actgcatcgg gtgctgccag acgatcagat cggcgcgaga cagcgcctcc
    35161 tgctcggcgg caatatcgat attgaagtca ggatagagtt gataaagaga gcgaatttcg
    35221 acgccttcca gcgtccttgc ctgttcaagc atccgtttat tcgcatggga atgatgcgga
    35281 tacggatgcg cataaattat aagaatcata ggtagcccgc gtgattacgc ctttgttttc
    35341 ccacaaagtg tagtcatata ttcacgcggc ttatagtcaa taatattgat tcagaaaaga
    35401 gagaaatctg aacaatttac cccattctct gcccgcggat cgtcatattg ctggtttgcg
    35461 ccagtgacat ccctcgagtc tccggggcaa acgctacgga aatcaacaag ccaaacagcg
    35521 agatacccgc ccccattagc atcgtgttac tgataccgta attattgata aagatcggca
    35581 gtgcccaggt cgaaacaatg gtgccaatac ggcttaagga cataatcacg cccacggcag
    35641 aggcgcggat atctgtcggg aagagttcat taggatagag ccactgcaaa ttacccggcc
    35701 cgccagagaa aaaggcatac accgcaaagg ccatcactac cagccagatc cccatatccg
    35761 ggatcagccc caaaaccgcc agcgccagcg tcatcatggc aaagctgcta atcaacaatg
    35821 gacgccgtcc ggcagtgttt agccacagca tcggcggaat acagccgagc ataaagaaca
    35881 ggctaatcac cacgttcccc agtgccgcgt ttttgccaac ccccaatccc aacaaaccaa
    35941 cgatttgcgg gccaaaggtg taaatggcga acattgggat cacctggcag gtccagatgg
    36001 cggcaacaaa cagaacaaaa ggaaaatggc ggcgattaaa cagatcgcga aaacgggttt
    36061 gctgcggctg ctcttcatcg aaagccaccg gttcgccaaa cagttttatc atcatctctt
    36121 cgcactcttt tactcgccct ttgcgtaata accagcgggg agattcaggc agttcgaatc
    36181 gaccaatcaa aatcaacaaa caggggatcg ccgcgctacc cagcatccag cgccagccgc
    36241 cttccacatc ataaagccag tagccgacca gatcggcgca ggtcgcgccg acataccaca
    36301 tggcggcgat aaagctgatg gaaaacgccc gctgacgggt actggagaac tcggtgatca
    36361 ttgaggtggc gatgggataa tctgcaccga tgacaatgcc gataagtacc cgcatcacca
    36421 acagttcgac aggggatgag acaaacatcg tcgccaccga tatcacgccg atggcgatga
    36481 tatcaatgag gaacattttg cgccgtccga ctttatcgga gatatagcca aacagcgatg
    36541 tgccaacgaa cagcccggcg agcgttcccg cacccagcaa gccaatccag tcagcgtcca
    36601 gtttcagcgc cggagtcagt tgctccagcg ccacgccaat cattaccagt acataaccat
    36661 ccagaaacgg accaccactt ccccacagca aaatgcggcg gtgaatagag gagaatttga
    36721 gatcgtcaaa gtttctggac ggttgcatgg gttattcctg ttttttgcaa tgagagtcag
    36781 tgcggcgtta cgtatcaaac caacatcagc cgtaacggaa ctccacacca aaggtgccgc
    36841 gcgggtattc ccactgttcc agcgccgacc ccagccccag aatgcgacag gtgccgcact
    36901 ccagacatcc ggcgtaatcg aagcgcacac tgccgtcatc ctgcttcttg tacagacctg
    36961 cggggcacgc tttcaccagc agctccagca cctgtttatc agcatcggcc ttcacaacaa
    37021 tgtgcggatg ctcttcatcg acattgaatt tattgacgcc cagtttgacg tccacattga
    37081 cgggagaagt cataaaacgg tcactccttt catgccatcc ttgattagat tgatgaagcc
    37141 cactttctta ccgtggcgga ggattttctt gcgcatcagt tctggcgcac tgccgtcaat
    37201 ggtgaacagg tcacgcgcca cgcccaccgc cagctccggg tagccgctga acatgcgtgg
    37261 gttatcaagg aacgccggta gtttctggta catgcgcata tcgcgcagcg ggccactctc
    37321 aagatgctga cgatattccg ccagtttttg cttactgaaa tcgtcgcttt tcatcgctga
    37381 aagcacggtt tttgctgcag cttccccggc ggcaatcgcc agatccatac cgcgaatggt
    37441 aaaaccgagg ttcatacaca ttccggcggc atcgccggca atcaatacgc cgtcaccgac
    37501 cagctccggc agcatgttga tgcctgcttc cggcactacg tgagcggaat attccaccag
    37561 cttgccaccc gcgatcagcg gtgcaacggc cggatgttgt ttgaaatctt ccagcatttg
    37621 cggcaccgat ttttttgcgt catgcagatg atgcagacca caaaccagcc ccagcgacag
    37681 ggtattttca ttggtataaa ggaagccgcc gcccatcagg ccatcggtgg gtgatcccgc
    37741 aaacagacaa gccgcgccct gattaccctg caactgaaaa cggtcttcga ttaccgactt
    37801 cggtaactcg atcagttcct tcacgccaac cgccacatcc gtcggtttaa cgcgttttgc
    37861 catccccaac ttttcggcaa ggatggaatt caccccgtca gcaaggatca ccgttttcgc
    37921 ttcaatcaca tcgccatcgg cttctacacc gacgactttg ccatcgcgct gtacgaggtt
    37981 atcgacacga atcccggtaa ttaactgcgc gcccgcttct tcggcctgct ccatcaacca
    38041 ggcatcaaat ttactgcgca aaacggagta agaacgctgg gatggcgagg tttcgtcacc
    38101 gttgcagtag tccatagtca tcgctgactt ttccgtcata aacgcgagtt tttcatgggt
    38161 gatcaggcgt tctacggggg cggagtcggc gaaaccagga ataatgtgtt ccagactgtg
    38221 ggcatagaga cgcccgccgg tgacgttctt ggcacctgcg gaattgccac gctcgataac
    38281 taacacttgc gcaccttcgc gggcgagcac cagtgcggca accgaaccgg caagccctgc
    38341 accgacgatg atggcgtcaa agatatcttc ggacataaga gctccctgtc agcggcaggg
    38401 ccaaaatgca gccctgccag agtggatcaa cgcgctaaag ctgcggtcag cgccggaagg
    38461 atcttcacgg cgtcgccaac aatgccgtaa tccgcgtact ggaagatcgg cgcattttta
    38521 tctttgttga tggcgaaaat ggtttgcgat gcattagcac caaccatgtg ctggatctgc
    38581 ccggagatcc ccaccgccag gtacagttca ggtttcagca tcaggttgga gataccgaca
    38641 tagcgttcgt gctccatcca tttttcgttt tccgccaccg gacgagaaca ggccaactcc
    38701 gcacctatcg ccttgcaaag ctgttctgcc agcgcaatgt tctctttgct gccaataccg
    38761 cgaccgacgc tgaccaccag acgggctttg tcgagatcga cgctgttgct ctggcgcgcc
    38821 tgggttgccg tgcgggtaat cgccacagcc ggagcctgcc actccacggt gtgcgtttcg
    38881 ccagtgcgtg acgcgtctgg ctgagccgca tcgaacgtgc cgctgctgat ggtcagtacc
    38941 gcatacggcg tggcaatgcg ttcttcgcca atcgccagac caccgtaaac catgtgtttc
    39001 actgtcgctt taccgtcctg tacgctgacg gtgctggcat cgttagacac cgccgcttta
    39061 aggcgataac ccagttttgc cgccagtaat ttgccgcgac gggtgtttgg cagcagcacc
    39121 aggccgtctg cgccgtgctg gcgaatagtg tcagccatga caccggcgta atcttcgatc
    39181 atccgatcgt ccggtttgcc gtttaatttc cagacatgat tagcgccgag ctggattgcc
    39241 tgtacgccgt cggcatcatt gaggacaaag gtgttgattt gattagctaa agcctgcgca
    39301 ccgttcatca gttccggcag acgagaaggg gtatcgctga atacccagac ttgagaaaac
    39361 gtgttcatag catcccctgt aattaaatga ctttgcgaag attttcagca aatgcggcga
    39421 tctgttcttc gccgtcgcct tcaatcacga tgcgctgacg ttcgcgctgt ttcggcgcag
    39481 caacctgttg ttcagaccag gcgtccacgg cgttaaaacc gatatccgcc gccgaccata
    39541 cctggactgg ctttttcgcc gcgccgagaa tggctttcat cgaaggaatt tgtggggagt
    39601 tgatatcagt ggaaacagca acaaccgcag gcagcggaat gcttaaggtt tcggtttcat
    39661 cttccagttc gcgctcaacg gtgagggtat ctgccgtcag ggaaataatt ttgctgacgc
    39721 cgttaactgc cggaatattg aggatttcgc ccaccagcag accaacctgc tgggcataaa
    39781 ggtcggaaga accatcgcca cagaggatca aatcaaagcc tgctttctgg gcggctgcag
    39841 ccagtgcgct cgccgtttgt tgcggcagtg cctgctcgaa ctggtcatca atcaccacaa
    39901 tcagttcatt cgggccgcgc gatagcacat ctttacgccc tttggcgttg gtcagggctt
    39961 taccgcccac acttaaggcc gtcacctgcg cctctgctgc ctgttgcttt agctggcaag
    40021 ccgcttcaat agcgttcaga tcgtattggc ttattttggc atcggctttg ctgaagtcta
    40081 atgaaccatc agcattattg accgcaatat cctgttcatc aggcacgcac ttatagcaag
    40141 taataatctt cattgcatct ccagaaatca tgaaggaaac gtcgttcata tcggtggtgt
    40201 taattacaga tatcacgccc tttaatttgg aaataaaact ttaatcacca atattgaaaa
    40261 tgtcacacgc agataaatcc aactttcaat attgttaagt tcctcaccaa tattgaaaac
    40321 atggcgtagc aaaaagaaat tttcaatatt gctttatgga tcaccaatat tgaaagtcag
    40381 atgctatttt tttactcaca acacaacata acaaactgat taataatcaa aattaacgag
    40441 aaaagcgccc tctattcacc cgaaacaaaa atgtgataac aatcacagaa tgcagcttat
    40501 tgaataccca ttatgagtta gccattaacg cgtccacga
//
LOCUS       DAPHDW010000027        40261 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.027, whole genome shotgun sequence.
ACCESSION   DAPHDW010000027 DAPHDW010000000
VERSION     DAPHDW010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 40261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40261
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.027"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            114..1478
                     /locus_tag="RH057_001403"
     CDS             114..1478
                     /locus_tag="RH057_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3127799.1"
                     /translation="MNDYKMTPGERRATWGLGTVFSLRMLGMFMVLPVLTTYGMALQG
                     ASEALIGIAIGIYGLTQAVFQIPFGLLSDRIGRKPLIVGGLAVFAAGSVIAALSDSIW
                     GIILGRALQGSGAIAAAVMALLSDLTREQNRTKAMAFIGVSFGITFAIAMVLGPIITH
                     KLGLHALFWMIAILATTGIALTIWVVPNSSTHVLNRESGMVKGSFSKVLAEPRLLKLN
                     FGIMCLHMLLMSTFVALPGQLADAGFPAAEHWKVYLATMLIAFGSVVPFIIYAEVKRK
                     MKQVFVFCVGLIVVAEIVLWNAQTQFWQLVVGVQLFFVAFNLMEALLPSLISKESPAG
                     YKGTAMGVYSTSQFLGVAIGGSLGGWIDGMFDGQGVFLAGAMLAAVWLAVASTMKEPP
                     YVSSLRIEIPADIAANEALKVRLLETEGIKEVLIAEEEHSAYVKIDSKVTNRFEIEQA
                     IRQA"
     gene            complement(1606..2097)
                     /gene="yajQ"
                     /locus_tag="RH057_001404"
     CDS             complement(1606..2097)
                     /gene="yajQ"
                     /locus_tag="RH057_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HDV3127800.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            2265..3176
                     /gene="panE"
                     /locus_tag="RH057_001405"
     CDS             2265..3176
                     /gene="panE"
                     /locus_tag="RH057_001405"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HDV3127801.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVYDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            3139..3729
                     /gene="yajL"
                     /locus_tag="RH057_001406"
     CDS             3139..3729
                     /gene="yajL"
                     /locus_tag="RH057_001406"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HDV3127802.1"
                     /translation="MSASALVCLAPGSEETEAVTTIDLLVRGGIKVTTASVASDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGAECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(3783..5231)
                     /gene="thiI"
                     /locus_tag="RH057_001407"
     CDS             complement(3783..5231)
                     /gene="thiI"
                     /locus_tag="RH057_001407"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HDV3127803.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLAIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPEVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSIDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNR
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            5437..5679
                     /gene="xseB"
                     /locus_tag="RH057_001408"
     CDS             5437..5679
                     /gene="xseB"
                     /locus_tag="RH057_001408"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HDV3127804.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            5679..6578
                     /gene="ispA"
                     /locus_tag="RH057_001409"
     CDS             5679..6578
                     /gene="ispA"
                     /locus_tag="RH057_001409"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HDV3127805.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQSLKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            6603..8465
                     /gene="dxs"
                     /locus_tag="RH057_001410"
     CDS             6603..8465
                     /gene="dxs"
                     /locus_tag="RH057_001410"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HDV3127806.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLAI
                     LNFGTLMPDAAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLGAAGMEAKIKAWLA"
     gene            8520..9494
                     /gene="yajO"
                     /locus_tag="RH057_001411"
     CDS             8520..9494
                     /gene="yajO"
                     /locus_tag="RH057_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HDV3127807.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSIDDSLRRLGMDYVDILQIHRWDYNTPIEVTLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHGWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKPEQIAELETPYKPHAVVGFK"
     gene            complement(9548..10066)
                     /gene="pgpA"
                     /locus_tag="RH057_001412"
     CDS             complement(9548..10066)
                     /gene="pgpA"
                     /locus_tag="RH057_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HDV3127808.1"
                     /translation="MTILPRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGTMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(10044..11021)
                     /gene="thiL"
                     /locus_tag="RH057_001413"
     CDS             complement(10044..11021)
                     /gene="thiL"
                     /locus_tag="RH057_001413"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HDV3127809.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLHDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFATP"
     gene            complement(11099..11518)
                     /gene="nusB"
                     /locus_tag="RH057_001414"
     CDS             complement(11099..11518)
                     /gene="nusB"
                     /locus_tag="RH057_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HDV3127810.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(11538..12008)
                     /gene="ribE"
                     /locus_tag="RH057_001415"
     CDS             complement(11538..12008)
                     /gene="ribE"
                     /locus_tag="RH057_001415"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HDV3127811.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(12097..13200)
                     /gene="ribD"
                     /locus_tag="RH057_001416"
     CDS             complement(12097..13200)
                     /gene="ribD"
                     /locus_tag="RH057_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HDV3127812.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVASMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQLLRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPIRIVIDSQNRVTPEHHIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGALLQAGLVDELIVYIAPKLLGSDARGLC
                     TLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(13204..13653)
                     /gene="nrdR"
                     /locus_tag="RH057_001417"
     CDS             complement(13204..13653)
                     /gene="nrdR"
                     /locus_tag="RH057_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HDV3127813.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDDVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            13804..14343
                     /locus_tag="RH057_001418"
     CDS             13804..14343
                     /locus_tag="RH057_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV3127814.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     KETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDADGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVEQLDFVRIHDIQPVMQ"
     gene            14642..15526
                     /gene="tsx"
                     /locus_tag="RH057_001419"
     CDS             14642..15526
                     /gene="tsx"
                     /locus_tag="RH057_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HDV3127815.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(15703..16050)
                     /locus_tag="RH057_001420"
     CDS             complement(15703..16050)
                     /locus_tag="RH057_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HDV3127816.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(16179..17150)
                     /gene="secF"
                     /locus_tag="RH057_001421"
     CDS             complement(16179..17150)
                     /gene="secF"
                     /locus_tag="RH057_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HDV3127817.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALAHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(17161..19008)
                     /gene="secD"
                     /locus_tag="RH057_001422"
     CDS             complement(17161..19008)
                     /gene="secD"
                     /locus_tag="RH057_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HDV3127818.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(19036..19368)
                     /gene="yajC"
                     /locus_tag="RH057_001423"
     CDS             complement(19036..19368)
                     /gene="yajC"
                     /locus_tag="RH057_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HDV3127819.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(19391..20518)
                     /gene="tgt"
                     /locus_tag="RH057_001424"
     CDS             complement(19391..20518)
                     /gene="tgt"
                     /locus_tag="RH057_001424"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HDV3127820.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSVYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(20574..21644)
                     /gene="queA"
                     /locus_tag="RH057_001425"
     CDS             complement(20574..21644)
                     /gene="queA"
                     /locus_tag="RH057_001425"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HDV3127821.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDDRSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            21737..22318
                     /gene="acpH"
                     /locus_tag="RH057_001426"
     CDS             21737..22318
                     /gene="acpH"
                     /locus_tag="RH057_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HDV3127822.1"
                     /translation="MNFLAHLHLAHLAESSLSGNLLADFVRGNPEESFPPDVVAGIHM
                     HRRIDVLTDNLPEVREAREWFRSETRRVAPITLDVMWDHFLSRHWSQLSPDFPLQEFV
                     CYAREQVMTILPDSPPRFINLNNYLWSEQWLVRYRDMDFIQNVLNGMASRRPRLDALR
                     DSWYDLDAHYDALETRFWQFYPRMMAQASHKAL"
     gene            complement(22323..24140)
                     /gene="malZ"
                     /locus_tag="RH057_001427"
     CDS             complement(22323..24140)
                     /gene="malZ"
                     /locus_tag="RH057_001427"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HDV3127823.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVSMHKQRSQQQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNRGTGGACHNPES
                     PWRDWYSFSDDGTALDWLGYASLPKLDYQSESLVNEIYRGEDSIVRHWLKAPWSMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYQRMIALRKKSQALRHGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEACEV
                     VLPASPLLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(24296..25669)
                     /gene="proY"
                     /locus_tag="RH057_001428"
     CDS             complement(24296..25669)
                     /gene="proY"
                     /locus_tag="RH057_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HDV3127824.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVWFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(25745..27064)
                     /gene="brnQ"
                     /locus_tag="RH057_001429"
     CDS             complement(25745..27064)
                     /gene="brnQ"
                     /locus_tag="RH057_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HDV3127825.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVAAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFRLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(27471..28766)
                     /gene="phoR"
                     /locus_tag="RH057_001430"
     CDS             complement(27471..28766)
                     /gene="phoR"
                     /locus_tag="RH057_001430"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HDV3127826.1"
                     /translation="MLERLSWKRLVLELLLCCFPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTHLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(28824..29513)
                     /gene="phoB"
                     /locus_tag="RH057_001431"
     CDS             complement(28824..29513)
                     /gene="phoB"
                     /locus_tag="RH057_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HDV3127827.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIEMQGLSLDPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            29703..30905
                     /gene="sbcD"
                     /locus_tag="RH057_001432"
     CDS             29703..30905
                     /gene="sbcD"
                     /locus_tag="RH057_001432"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HDV3127828.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIITSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGASKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEELDESQQQRLQHLFTTTLH
                     TLAGEHEA"
     gene            30902..34045
                     /gene="sbcC"
                     /locus_tag="RH057_001433"
     CDS             30902..34045
                     /gene="sbcC"
                     /locus_tag="RH057_001433"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HDV3127829.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVALLTPEQV
                     QSLTASLQVLTDEEKQLLTAQQQEQQSLNWLTRLDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNAALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGATLRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDELPHSEETVALENWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHGRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            34171..35355
                     /gene="araJ"
                     /locus_tag="RH057_001434"
     CDS             34171..35355
                     /gene="araJ"
                     /locus_tag="RH057_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HDV3127830.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMGVLTELAHNVGISIPAAGHMI
                     SYYALGVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQEFSWRYTF
                     LLIAVFNIAVMASVYFWVPDIRDEAKGKLREQFHFLRSPAPWLIFAATMFGNAGVFAW
                     FSYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIV
                     LALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFN
                     LGSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADTPVLAKP
                     LG"
     gene            complement(35498..36406)
                     /gene="mak"
                     /locus_tag="RH057_001435"
     CDS             complement(35498..36406)
                     /gene="mak"
                     /locus_tag="RH057_001435"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HDV3127831.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNANSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRAHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIISLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVRKAKHGDSSGVRGAAWLWPQE"
     gene            36531..37442
                     /gene="rdgC"
                     /locus_tag="RH057_001436"
     CDS             36531..37442
                     /gene="rdgC"
                     /locus_tag="RH057_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HDV3127832.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWIDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(37520..37603)
                     /locus_tag="RH057_001437"
     CDS             complement(37520..37603)
                     /locus_tag="RH057_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127833.1"
                     /translation="MTQRPWSKLQHKTHNIAALKIIARRSE"
     gene            complement(37600..37941)
                     /locus_tag="RH057_001438"
                     /pseudo
     CDS             complement(37600..37941)
                     /locus_tag="RH057_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(38089..38373)
                     /gene="ppnP"
                     /locus_tag="RH057_001439"
     CDS             complement(38089..38373)
                     /gene="ppnP"
                     /locus_tag="RH057_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HDV3127834.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPSHSEFHLQVAEPTSYLCRYL"
     gene            complement(38445..39122)
                     /locus_tag="RH057_001440"
     CDS             complement(38445..39122)
                     /locus_tag="RH057_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HDV3127835.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLTVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(39380..39571)
                     /gene="yaiA"
                     /locus_tag="RH057_001441"
     CDS             complement(39380..39571)
                     /gene="yaiA"
                     /locus_tag="RH057_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HDV3127836.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(39621..40145)
                     /gene="aroL"
                     /locus_tag="RH057_001442"
     CDS             complement(39621..40145)
                     /gene="aroL"
                     /locus_tag="RH057_001442"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HDV3127837.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAPSTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
BASE COUNT         9978 a        10788 c        10180 g         9315 t
ORIGIN      
        1 aaacatacct gacacacaaa ttactctcct ctgtaatatt ttctcattaa cactctgttt
       61 acccaacatg cgctccgcac tacactatgt gggcattttt ttaaggggtg gtaatgaacg
      121 attataaaat gacgccaggt gagaggcgcg cgacctgggg tttagggacc gtattctcgt
      181 tgcgcatgct gggcatgttt atggttctgc cggttctgac cacgtacggc atggctctgc
      241 aaggtgccag cgaagcatta atcggtattg ccattggtat ttatggtctg actcaggccg
      301 tttttcagat tccgtttggc ctgctttccg accgcattgg tcgcaaacca ttaattgtcg
      361 gtgggctggc ggtgtttgcc gccggtagcg ttatcgctgc gctttccgac tccatctggg
      421 gaattattct gggccgggcg ctacaaggct ctggagcaat tgccgccgcc gttatggcgc
      481 tgctttccga tctcacgcgc gaacaaaacc gcaccaaagc gatggcgttt atcggcgtga
      541 gctttggcat tacctttgcc attgcgatgg tgcttggccc gatcatcact cacaaacttg
      601 ggctgcacgc gctgttctgg atgatcgcta ttctggcaac aaccggcatt gcgttgacca
      661 tttgggttgt gcccaacagt agcactcacg tacttaatcg tgagtccgga atggtgaaag
      721 gcagtttcag taaagtgctg gcggaaccgc ggctgctaaa actcaacttt ggcattatgt
      781 gtctgcacat gcttctgatg tctacgtttg ttgccctgcc cggacagcta gctgatgcgg
      841 ggttcccggc ggctgaacac tggaaggtct atctggcgac gatgttaatc gcctttggct
      901 cggtcgtgcc tttcattatc tacgctgaag ttaagcgcaa aatgaagcaa gtctttgtct
      961 tctgcgtcgg gttgatcgtg gttgcggaaa ttgtgttgtg gaacgcacaa acgcagttct
     1021 ggcaactggt ggtcggcgtg cagcttttct ttgtggcgtt taatttgatg gaagcccttc
     1081 tgccctcact tatcagtaaa gagtcgccag caggttacaa aggtacggcg atgggtgtct
     1141 actccaccag ccagtttctt ggcgtggcga ttggcggttc gctgggcggc tggattgacg
     1201 gcatgtttga cggtcagggg gtatttctcg ctggcgcaat gctggccgca gtgtggctgg
     1261 cagtcgccag taccatgaaa gaaccgccgt atgtcagcag tttgcgcatt gaaatcccgg
     1321 cggacattgc cgcaaacgaa gcgttaaaag tgcgtttgct ggaaactgaa ggcatcaaag
     1381 aagtgttgat tgcagaagaa gaacattcag cttatgtgaa aatcgacagc aaagtgacga
     1441 atcgctttga gatagaacag gcaattcgtc aggcataaaa aacgcggcga attgcctgct
     1501 gcgctacgct tatcaggcct acaaagtttc tgaattacaa acctttgtag gccgaatcag
     1561 gcgttcatgc cgcatctgac atgaacaaaa cgcacatagt cgcgattaat cgcggaagtt
     1621 tttgaactgg aacggctgac cgagatcgcc accacgtacc atcgccatga cagcctgcaa
     1681 atcatcacga gatttgcccg ttacgcggat ttcatcgccc tgaatttgcg cctgcacttt
     1741 cagtttgctg tctttgatca tcttgacgat tttcttctgg gtcgcgctct caatgccctg
     1801 tttcagtttc gcttccacaa accaggtttt accgctatga acgatattct ccggtacatc
     1861 cagcgaactg ccttcaatgc cgcgcttcag cagcttggca cgcagaatat ccagcaactg
     1921 attgacctgg aaatcggact cgctcaacac tttgatggtt ttgctggcgt cgttcagctc
     1981 aaatgaggct tcaacgttac ggaagtcaaa acgggactcc acttcgcggc tcgcgttatc
     2041 gaccgcgtta cgtgcttcct gaagatcaac ttcagagaca atatcgaaag atggcatctt
     2101 ttcctctccc ttcatttttg atgcgaagca taatacccgc aaagttaaat aaccgacagt
     2161 cctgcaccag gacgtatccc ttaattgtgc atagtcgcaa cagcatgcac aattaaggga
     2221 tagccttatt gaacacccgg agtggttgcg ggtgaggagg aacaatgaaa attaccgtat
     2281 tgggatgcgg tgccttaggg caattatggc ttacagcact ttgcaaacag ggtcatgaag
     2341 ttcagggctg gctgcgcgta ccgcaacctt attgtagcgt gaatctggtt gagacagatg
     2401 gttcgatatt taacgaatcg ctgaccgcca acgatcccga ttttcttgcc accagcgatc
     2461 tgctcctggt gacgctgaaa gcatggcagg tttacgatgc cgtcaaaagc ctcgcgtcca
     2521 cactgcctgt aaccacgcca atactgttaa ttcacaacgg catgggcacc atcgaagagt
     2581 tgcaaaacat tcagcagcca ttactgatgg gcaccaccac ccatgccgcc cgccgcgacg
     2641 gcaatgtcat tattcatgtg gcaaacggaa tcacgcatat tggtccggca cggcaacagg
     2701 atggcgatta cagttatctg gcggatattt tgcaaaccgt gttgcctgac gttgcctggc
     2761 ataacaatat tcgcgccgag ctgtggcgca agctggccgt caactgtgtg attaatccac
     2821 tgaccgccat ctggaattgc ccgaatggtg aattacgtca tcatccgcaa gaaattatgc
     2881 agatatgcga agaagtcgcg gcagtgatcg agcgcgaagg gcatcatact tcagcagaag
     2941 atttgcgtga ttacgtgatg caggtgattg atgccacagc ggaaaatatc tcgtcgatgt
     3001 tgcaggatat ccgcgcgctg cgccacaccg aaatcgacta tatcaatggt tttctcttac
     3061 gccgcgcccg cgcgcatggg attgccgtac cggaaaacac ccgcctgttt gaaatggtaa
     3121 aaagaaagga gagtgaatat gagcgcatcg gcactggttt gcctcgcccc tggtagtgaa
     3181 gagactgaag ccgtcaccac tatcgatctg ctggttcgcg gcggtatcaa agtcaccact
     3241 gccagcgtcg ccagcgatgg caacctggcc attacctgct cgcgcggcgt gaagctgctg
     3301 gcggatgcgc cgctggtcga ggtggctgat ggcgaatatg acgtgatcgt actgcctggt
     3361 ggcattaaag gcgcagagtg ttttcgcgat agtaccctgc tggttgaaac cgttaaacag
     3421 ttccaccgtt ccgggcgtat cgtcgcggct atttgcgccg cgccagccac cgtgctggtg
     3481 ccgcacgata tcttcccgat tggtaatatg accggcttcc cgacgctgaa agacaaaatt
     3541 cccgctgaac aatggcagga caagcgcgtc gtctgggatg cacgggtaaa attgctgacc
     3601 agccaggggc cggggacagc tatcgacttt ggtctgaaaa ttatcgacct gctggttggg
     3661 cgcgagaaag cccatgaagt ggcatcacaa ctggtgatgg cggcagggat ttataattat
     3721 tacgagtagt gtcggatgcg gtaagcgtcg catctgacca gatgcgacgt tacaaccaca
     3781 aattacgggc gatatacctt cacattgtta aagccctgct cgcgcagata gagcgcctgc
     3841 agacggctca tcaccccgcg ctcacaccac agtagccagg ttctgttctg gtcgagatcg
     3901 ccaaacttgg tgctcagttt atagaacggc agagaaacta catcaatccc ttcgactttc
     3961 agtggcttat cttcctgttc atcgatagaa cggatatcga ggatcacgtc gttcgggccg
     4021 aagccattga cggtttccac ttccaccact tcctgctcgg tctgctgggc gatttcgcgg
     4081 atatcaacgt tattcgcttc ttcaaccact ttatcgagaa tgctgaagtc gaatttctct
     4141 tcttccgctt caatcttcga cttaaccgct ttcaccgtcg ggcttttgga gataacaccg
     4201 caatattccg gcatcgtgcg agcaaagtct tcggtgccaa tctggcgagc caggttgatg
     4261 atgtgctctt tgtcgtaaga aatcagcgga cgcaggatca gcgtatcgga gacgttatca
     4321 atcaggcgca ggttggtcag cgtctgactg gacacctggc cgagcgcttc gccggtgacc
     4381 agcgcctgta cgccgtaacg ttcagccact ttagacgcgg cacgcaccat catacgtttg
     4441 aggataacgc ccatctgacc gtcgtcgatt ttctcgagaa tttccccgac gaccggttcg
     4501 aaattaatag cgacaaaacg cacgcggtgg gagctgccaa aacggttcca cagataatgc
     4561 gccacctgac gcacgccaat ttcatgcgcc gcgccgccaa ggttaaagaa acagtaatgc
     4621 acgcggcagc cgcgacgcat caacatataa ctggaaacac cggagtcgaa accaccggaa
     4681 atgagcgaca gcacatcttc ctgggtgccg atcgggaaac cgccaatacc ttcgtagcgg
     4741 cctttaatca gcaggagacg atcgtcttcc acttccagat ggacagtcac ttccggattg
     4801 gtcagcttca cgcgcgcgga ctcaatatgc tgatttaaac cgccgccgac gtaacgttcc
     4861 acatcaatcg agctaaaatc atgtttgcca cggcgcttca cgcgtacgca gaaggttttg
     4921 ccttccagct gatcgcgata ctgaaccaac gctttctcaa aaatatcgtg catgtcggta
     4981 aacggcacgt cttcgacttc gagaatatgg tggatacccg gaatacgggt cagggcgtcg
     5041 cgaatagcca gacgctggtt ttcatctttt gcgcgaactt cgatgttatc ccagtggcgg
     5101 acgacagcga gcgtctcatc atagtgcttt aaaacgttac gaatgttccc ggtaaggatt
     5161 tttataaagc gcaagcgcac agattggctt ttgatggtga tttccgggaa caatttaatg
     5221 ataaacttca tggcggcaat ggttcgttgg caagccttaa gcgacttgta tagggaaaaa
     5281 tacagcagcc cacacctgcg gctgcatcca ggcgcggaag tataccacta acatcgcttt
     5341 gctgcgcaca tcaccttacc attgcgcgtt atttgctatt tgccctgagt ccgttaccat
     5401 gacggggcga aaaatattga gagtcagaca ttcattatgc cgaagaaaaa tgaggctccc
     5461 gccagctttg aaaaggcgct gagcgagctg gaacagattg taacccgtct ggaaagtggc
     5521 gacctgccgc tggaagaggc gctgaacgag ttcgaacgcg gcgtgcagct ggcacgtcag
     5581 gggcaggcca aattacaaca agccgaacag cgcgtacaaa ttctgctgtc tgacaatgaa
     5641 gacgcctctc taaccccttt tacaccggac aatgagtaat ggactttccg cagcaactcg
     5701 aagcctgcgt taagcaggcc aaccaggcgc tgagccgttt tatcgcccca ctgccctttc
     5761 agaacactcc cgtggtcgaa accatgcagt atggcgcatt attaggtggt aagcgtctgc
     5821 gacccttcct ggtttatgcc accggtcata tgtttggcgt tagcacaaac acgctggacg
     5881 cacccgctgc cgccgtagag tgtatccacg cttactcatt aattcatgat gatttaccgg
     5941 cgatggatga tgacgatctg cgtcgcggtt tgccgacctg ccatgtgaag tttggcgaag
     6001 caaacgcgat tctcgctggc gacgctttac aaacgctggc gttctcgatt ctaagcgatg
     6061 ccaatatgcc ggaagtgtcg gatcgcgaca gaatttcgat gatttctgaa ctggcgagcg
     6121 ccagcggtat tgccggaatg tgcggtggtc aggcattaga tttagacgcg gaaggcaaac
     6181 acgtacctct ggacgcgctt gagcgtattc atcgtcataa aaccggcgca ttgattcgcg
     6241 ccgccgttcg ccttggtgca ttaagcgccg gagataaagg gcgtcgtgct ctgccagtac
     6301 tcgacaagta cgcagagagc atcggccttg ccttccaggt tcaggatgac atcctggatg
     6361 tggtaggaga tactgcaacg ttgggaaaac gccagggtgc cgaccagcaa cttggtaaaa
     6421 gtacctaccc tgcacttctg ggtcttgagc aagcccggaa gaaagcccgg gatctgatcg
     6481 acgatgcccg tcagtcgctg aaacaactgg ctgaacaatc actcgatacc tcggcactgg
     6541 aagcgctagc ggactacatc atccagcgta ataaataaac aataagtatt aataggcccc
     6601 tgatgagttt tgatattgcc aaatacccga ccctggcact ggtcgactcc acccaggagt
     6661 tacgactgtt gccgaaagag agtttaccga aactctgcga cgaactgcgc cgctatttac
     6721 tcgacagcgt gagccgttcc agcgggcact tcgcctccgg gctgggcacg gtcgaactga
     6781 ccgtggcgct gcactatgtc tacaacaccc cgtttgacca attgatttgg gatgtggggc
     6841 atcaggctta tccgcataaa attttgaccg gacgccgcga caaaatcggc accatccgtc
     6901 agaaaggcgg cctgcacccg ttcccgtggc gcggcgaaag cgaatatgac gtattaagcg
     6961 tcgggcattc atcaacctcc atcagtgccg gaattggtat tgcggttgct gccgagaaag
     7021 aaggcaaaaa tcgccgtacc gtctgtgtaa tcggcgatgg cgcgattacc gcaggcatgg
     7081 cgtttgaagc gatgaatcac gcgggcgata tccgtcctga tatgctggtg gtcctcaacg
     7141 acaacgaaat gtcgatttcc gaaaatgtcg gcgcgctcaa caaccatctg gcgcagctgc
     7201 tttccggtaa gctttactct tcactgcgcg aaggcgggaa aaaagttttc tctggcgttc
     7261 cgccaattaa agagctgctc aaacgtaccg aagaacatat taaaggcatg gtagtgcctg
     7321 gcacgttgtt tgaagagctg ggctttaact acatcggccc ggttgacggt cacgatgtgc
     7381 tggggcttat caccacgctg aagaacatgc gcgacctgaa aggcccgcag ttcctgcata
     7441 tcatgaccaa aaaaggtcgt ggttatgaac cggcagaaaa agaccccatc actttccacg
     7501 ccgtgcctaa atttgatccc tccagcggtt gtttgccgaa aagtagcggc ggtttgccga
     7561 gctattcaaa aatctttggc gactggttgt gcgaaacggc agcgaaagac aacaagctga
     7621 tggcgattac tccggcgatg cgtgaaggtt ccggcatggt cgagttttca cgtaaattcc
     7681 cggatcgtta cttcgacgtg gcaatcgccg agcaacacgc ggtgaccttt gccgccggtc
     7741 tggcgattgg tgggtacaaa cccattgtcg cgatttactc cactttcttg caacgcgcct
     7801 atgatcaggt gctgcatgac gtggcgattc aaaagctccc ggtcctgttc gccatcgacc
     7861 gcgcgggcat tgttggtgct gacggtcaaa cccatcaggg cgcttttgac ctctcttacc
     7921 tgcgctgcat accggaaatg gtcattatga ccccgagcga tgaaaacgaa tgtcgccaga
     7981 tgctctatac cggctatcac tataacgacg gcccgtccgc ggtgcgctac ccgcgcggta
     8041 acgcggttgg cgtggaactg acgccgctgg aaaaactgcc aattggcaaa ggcattgtga
     8101 agcgtcgtgg cgagaaactg gcgatcctta actttggtac gctgatgcca gacgcggcga
     8161 aagtcgctga atcgctgaac gctacgctgg tcgatatgcg ttttgtgaaa ccgcttgatg
     8221 aagcgttaat tctggaaatg gccgccagcc atgaagcgct ggtcaccgta gaagaaaacg
     8281 ccattatggg cggcgcaggc agcggcgtga acgaagtgct aatggcccat cgtaaaccag
     8341 tacccgtgct gaacattggc ctgcctgact tctttattcc acaaggaact caggaagaaa
     8401 tgcgcgccga actcggcctc ggtgccgccg gtatggaagc caaaatcaag gcctggctgg
     8461 cataatccct actccactcc tgctatgctt aagaaattat tcatggcagg agtggcagca
     8521 tgcaatacaa ccccttagga aaaaccgacc ttcgcgtttc ccgactttgc ctcggctgta
     8581 tgacctttgg cgagccagat cgcggtaatc acgcatggac actgccggaa gaaagcagcc
     8641 gtcccataat taaacgtgca ctggaaggcg gcataaattt ctttgatacc gccaatagct
     8701 attccgatgg cagcagcgaa gagatcgttg gtcgcgcact gcgggatttc gcccgtcgtg
     8761 aagacgtggt cgttgcgacg aaagtgttcc atcgcgttgg tgatttaccg gaaggattat
     8821 cccgtgcaca aattttgcgc tctatcgacg acagcctgcg acgtctcggc atggattatg
     8881 tcgatatcct gcaaattcat cgctgggatt acaacacgcc gatcgaagtg acgctggaag
     8941 ctctgaacga cgtggtaaaa gccgggaaag cgcgttatat cggcgcgtca tcaatgcacg
     9001 cttcgcagtt tgctcaggca ctggaactcc aaaaacagca cggctgggcg cagtttgtca
     9061 gtatgcagga tcactacaat ctgatttatc gcgaagaaga gcgcgagatg ctgccactgt
     9121 gttatcagga gggcgtggcg gtgattccgt ggagcccgct ggcgcggggg cgactgacgc
     9181 gtccgtgggg agaaactacc gcacgactgg tgtctgatga ggtggggaaa aatctctata
     9241 aagaaagcga tgaaaatgac gcgcagatcg cagagcggtt aacgggcgtc agtgaagaac
     9301 tcggtgcaac acgagcacaa gttgcgctgg cctggttgtt gagtaaaccg ggcattgccg
     9361 caccgattat cggtacatcg cgggaagaac agcttgatga gctgctgaac gcggtggata
     9421 tcactttgaa gccagaacag attgccgaac tggaaacgcc gtataaaccg catgcggtcg
     9481 taggatttaa ataagagtcc aggcctgatg agacgtgaca agcgtcacat aaggcactgg
     9541 tgcacaacta cgacagaata cccagcggcc agtgatgacc gataaagtac aggatacctg
     9601 cggagatcac cccggcgaca atatcgtcga tcatgatccc catgccgcca tgtacattgc
     9661 gatcaaacca gcggatcggc cacggcttcc acatatccag aatacggaaa atcacaaacc
     9721 cggcggcaac ccactgccag tcattggtcg gcagcgccat gagcgtgatc cacataccaa
     9781 taaattcgtc ccagacaatg ctgccgtgat cgtgcacgcc catatctttt gcggtctgat
     9841 gacaaagata gacgccgata cagatcccca gcatcaccac cagcgagtag agctgccagg
     9901 gcaaaaaggt catcagatac cagaacggaa tcgctgccag cgagcccatc gtcccaggaa
     9961 cgatcgggct taatccactt ccgaatccga cagcaagcag atgccacgga ttactcatct
    10021 tcaggcgact tttcgcgaca tctttatggc gtggcaaaat ggtcatatcc tttccagtca
    10081 aacgtgacag gttcgccgtc acgaataaaa caaagccctt cgatatcggc ggtcatctgc
    10141 ccgatacagg taaacggtac gcccaggtgt ccgagagcca catccagcgc gccacggttc
    10201 agttccggca cagtgaaaca caactcgtaa tcttcaccgc cagagagcgc ccagcgcagc
    10261 gcctgttcag gttcaacatg gcgagaaagc gcatcagaaa acggcagcaa tgccaggtca
    10321 atacgtgcgc cgcagtcgct ggctttcacg atatgcccga gatcggaaat cagaccgtca
    10381 gagagatcga tagctgaatt tgccagatcg cgcagtgcct gtccctgtaa aatacgcggc
    10441 gatggacgga gatgacgttt gatcaagtag tccgcatctt tagcatcggc aacctgcaaa
    10501 cggttttgca aaatcgccag cccggcggcg ctatcgcccg gtgtaccggt cacatagatc
    10561 cagtcacccg gtttcgctcc agagcgcgtt aaggctcgtc ccatcggaac aaagccgtgg
    10621 atacccaacg tcattgataa tggcccacgc gtggtatcgc cgccaatgag ttgcatatcg
    10681 taataattga gaagatcaaa caaactgtcg ctgaaggact caagccacgc ttcgtctacg
    10741 tccggtaagg ttaatgccag cgtcagccag gccggatcgg cccccatcgc tgccagatcg
    10801 cttaggttca ccgccagtgc tttatacgcc agatcagcag gatcgatatc atggaggaag
    10861 tggttgcccg ccaccagcgt atcagtgctg atcgccaggg tctgtttctc ggggatattg
    10921 agaagagcgc aatcgtcgcc gatgcccagt tcgacatcaa gacgagaact tcttacacgg
    10981 tcaaaataac gggcaatcag ggagaactcg ccacatgcca tacgttatgc ctcagcaggt
    11041 aaaaaagaaa aggccggcga tcgcggaacg gtcttccgtg aatctaccgg cctggatatc
    11101 actttttgtt agggcgaatc acaggtgctg ctttatcgag tacgccgttg acgaacttat
    11161 ggctgtcttc tgcgccgaac gatttcgcca gttcgatcgc ttcgttaatg gccactttgt
    11221 atggcacatc gctacgttta gacagctcgt acagcgcaat gcgcagtact gctttttcta
    11281 cctgtcccag ttcttccagc aggcgggaca ggtatggctt catcagtccg tcgaggtatg
    11341 cggtgttagt cgccaccccg gccagcagct cacggaagta caggacgtca acgtctttta
    11401 catcctgttc agccaggaac tggtattcaa catcagcgat gtcgttctgg gacaactgcc
    11461 aggagtagag cgcctggacg gcacactcac gagcgcggcg acgagcagca ggtttcacgg
    11521 atttcccctt actaatttca ggccttgatg gctttcaata cattaatcat ttcaagcgcg
    11581 gtcagtgcag cttctgcacc tttgttgcca gctttggtgc cagcacgttc gatcgcttgt
    11641 tcaatgcttt cagtggtcag aaccccaaaa gcaaccggaa tttcgctgtc ctgggcaaca
    11701 tgcgccaggc cgttgcttgc accaccagcg acatattcaa agtgggcagt gccaccacga
    11761 ataaccgtac ccagcgcaat caccgcgtcg tatttaccgg ttttagccag tgcacccgcc
    11821 gccagcggca gctcataggc accaggcacc caaacaacgg taatgttttc atcttttacc
    11881 tgaccgatac gtttcagtgc gtcaattgca ccttccagca ggctgtcatt gataaagttg
    11941 ttgaaacgcg cgatggtgat ggcgacgcga gcgtccgggg tagcaacgtt agcttcaata
    12001 atgttcattt tcttccttcg ggttcgagta tggccccgca ggggggcgga ttttagcata
    12061 atatttcgtg cgctgcttcc ctttcgagcc gggagatcat gcacccacta aatgcaggca
    12121 aacatccggg cctacatgac gtatctcttt gaatttaaat tggggggcgt cggctaattt
    12181 ctcaagccct ggcagcgtgc ataatccgcg ggcgtcgctg cctaatagtt taggtgcgat
    12241 atagacaatc agctcatcga ctaaacccgc ctgcagcaat gcgccagcga gcgttggccc
    12301 cgcttccacc cagatgctgt taatttgctg tttacccagt tgcatcatca gtacaaccag
    12361 atccagatga cctttatgct ctggaatcag caaggtacgc accgtttccg gccactcacg
    12421 agaatcttcc tgcgtacgcg cgaaccaggt ttcgccgggc tgctgcacaa tgtgatgttc
    12481 cggcgtcacg cgattttggc tatcaatcac aatacgtatc ggctggcgga gattttgttg
    12541 cgggtagagc gcctgagttt gttcatccag ttcggaccaa cgcaccgtta aggcaggatc
    12601 atccgccagc accgtggcgc tgctggttaa aatggcatga ctttgcgcgc gcagtagttg
    12661 tacatcacgc cgcgcctggg gcgaagtgat ccactggctt tcgccgctcg ccatcgccgt
    12721 gcgaccatca agcgatgcgc caagttttaa ctgaatataa ggaaagccgg tgcgcatccg
    12781 cttgagaaag cctttattca attgctcggc ttcactcatc atcaggccgt ggctgacgtc
    12841 aatgccagcc tgttgcagac ggtaaagtcc acgcccagcg acctgcgggt taggatcttg
    12901 catcgaggca accacgcgcg ctacgccagc ggcgatgagt gcgtcacagc acggtggcgt
    12961 acgaccatga tggctacagg gttcgagtgt cacatacgcc gttgcgcctt tggctttttc
    13021 acccgccata cgcaacgcgt gtacttcggc atgtggttca cccgctcgtt ggtggtaacc
    13081 ttcaccgaca atttcgccat ctttgacaat gacgcacccg acattcgggt tggggtgggt
    13141 ggtaaaacgt cctcgttgcg ccagctttag cgcccgcgcc atgtaatact cgtcctgcac
    13201 ggcttagtcc tccaggcgcg cgatctcttc gccaaattct ttgatatctt cgaaactgcg
    13261 gtagacagag gcaaaacgga tataggcgac tttatcgagc tttttcaatt gctccatcac
    13321 cagattgcca atcatcttgc tcggcacttc gcgctcaccg gtggcgcgca gctgcgattt
    13381 aatatgattg attgccattt cgacgtcatc ggaactcacc ggacgttttt ccagcgcccg
    13441 cagcattccg ctacgcaatt tctcttcatt aaacggttca cgcacgtcgt tgctttttac
    13501 aacacgcggc ataaccagct ccgccacttc aaaggtggtg aaacgttcat tacacaccag
    13561 acactgccga cggcggcgta cggatgaacc ctcgcccacg agacgagagt caattacctt
    13621 agtgtccacg gcgaaacaga atgggcaatg catacggcgt cctgaccagg tggttaacag
    13681 aaatctattt taccctgaac tgacgataca acaaaggcgc aacgcttttg agataaagga
    13741 tcgatgcttt cgctgctttt accctctacc attaggctaa ttgcgacgag aaagggacga
    13801 cctatgaata ctaacgtttt tcgactgctc ctgctgggaa gcctgttcag ccttagcgcc
    13861 tgtgtgcagc agagcgaagt gcgacagatg aaacacagcg tcagcacgct gaaccaggag
    13921 atgacgcaac tcaataaaga aacggtcaaa atcactcagc aaaacaggct gaatgcaaaa
    13981 tccagcagtg gggtttacct tctgccagga gcgaaaacac cggcaagact ggaaagccag
    14041 atcggtactt tacgtatgtc gctggtgaat attacgcctg atgcagatgg caccaccctg
    14101 acgctacgta ttcagggcga gtcgaatgat cccttgcccg cattcagcgg aactgttgaa
    14161 tatgggcaaa ttcagggaac aatagacaac tttcaggaaa tcaatgtgca gaatcaattg
    14221 attaatgccc ctgccagcgt cctcgccccc agcgatgttg atattccgtt acagttaaag
    14281 ggtatttctg tagaacagtt agacttcgta cgtatccacg atattcaacc tgttatgcag
    14341 taaacgtatt tcggggcgat tttgtgcgtc tcgcaacatc tttccccgtc attttgttac
    14401 tccgcttaca tcaccctgat tgatagtaaa agtttgcaac aagggtgaaa gtcagtacaa
    14461 tccctgcccg aatgtgtgta aacgtgaacg caatcgatta cgtaaatgat agaactgtga
    14521 aacgaaacat atttttgtga gcaatgattt ttataatagg ctcctctgta tacgaaatat
    14581 ttagaaacgc aatttgcgcc tttttcactc ccgcaaggga ttttcaaaca gtggcataca
    14641 tatgaaaaaa acattactgg cagccggtgc ggtactggcg ctctcttcgt cttttactgt
    14701 caacgcagct gaaaacgaca aaccgcagta tctttccgac tggtggcacc agagcgttaa
    14761 cgttgtcgga agctatcaca cccgtttcgg accgcagatc cgcaacgata cctaccttga
    14821 gtacgaagca ttcgctaaaa aagactggtt cgacttctat ggttatgcgg atgcgccggt
    14881 attcttcggc ggtaactccg atgctaaagg tatctggaac cacggttctc cgctgtttat
    14941 ggaaatcgaa ccacgtttct ccatcgacaa gctgaccaat actgacctta gcttcggtcc
    15001 gttcaaagag tggtacttcg cgaacaacta catttacgac atgggtcgta ataaagatgg
    15061 tcgccagagc acctggtaca tgggtctggg taccgacatc gatactggtc tgccgatgag
    15121 cctgtccatg aacgtctatg cgaaatacca gtggcagaac tatggcgcag cgaacgaaaa
    15181 cgagtgggac ggttaccgtt tcaaaattaa atactttgtg ccgattaccg atctgtgggg
    15241 cggtcagctg agctacatcg gcttcaccaa cttcgactgg ggttccgatt taggggatga
    15301 cagcggtaac gcaatcaacg gtattaagac ccgtactaat aactctatcg cttccagcca
    15361 tattctggct ctgaactacg atcactggca ctactctgtc gtagctcgtt actggcacga
    15421 cggtggtcag tggaacgacg atgcagaact gaacttcggc aacggcaact tcaacgttcg
    15481 ctctaccggc tggggtggtt acctggtagt aggttacaac ttctgattat gaaaatgccg
    15541 ggatttattc ccggcatttc agattgttaa caacgtgcgc tttgttcatg ccggatgcgg
    15601 cgtaaacgct ttgtccgacc cacaaaagca cgtaaattca atatattgct gaaataacgt
    15661 agtcctgata agcgtagcgc atcaggcaat tttacgtttt aatcacttct tcttgttcat
    15721 catcgctttc aggtcagcga atgggttgta cttcgcttca ccgacatctt tctgcgcatc
    15781 ttcccctgcg ataacggtcg taccatactg atccgcttcg gtatatttcg aatgctcatg
    15841 atcgtggcaa tagagacaaa gcaattccca gttactgcca tcttccgggt tattggtatg
    15901 gtcgtgatca atgtggtgaa cagtaagttc acgcaggttg gaataaacaa actcgcggga
    15961 acagcgaccg cagacccacg gatatatttt taatgctttt tcgcgatagc cgctttctaa
    16021 ccgcgcatag ttttttggga tgatagccat gaagagttac ctggatgaaa tgtagggtta
    16081 cgcgcagttt cccaagatgc ggggatttta gcaatctcca tacatcaaaa aaaatcccga
    16141 tcttctgacc gggattttca acatcaacgg gaacttgatt acggcagaat tgacggctga
    16201 tccgcccctt ctttttccac tttctgctgc aacatgtgtt cgcgcttcat acccagtttc
    16261 agagccaacg cagatgccac atagatggaa gatgcagtac cgatggaaac accgataagc
    16321 atggtcagcg agaagccttc cagtaccgga ccaccgaaga ggtacagcat caggataacc
    16381 atcaaggtag taccggatgt gatcaaggta cggtgcagcg tctgggtcaa ggacacgtta
    16441 aagatttcgt aaggcgtacc gcgacggatc ttgcggaagt tttcacgaat acggtccgat
    16501 accacgatac tgtcgttaag cgagtaaccg ataaccgaca tcaacgatgc cacaatggtc
    16561 aggtcaatct cgatatggaa taacgacaaa atacccagcg taatgataac gtcgtgcgcc
    16621 agcgcaataa cgacccctgc cgccagtcgc cactcaaagc gaaaacctac gtacacaagg
    16681 atagacagca gcgccgccat caacgccatc gcaccggttt gcgcaaggtc tgcccccacg
    16741 ctcggaccga cgaactcaat acgcttcact gctgcattct gattggtgga ttcgttaatc
    16801 accttcagaa cctggctgcc cagcacctga ccgccggttt cgccttcagc aggcggcata
    16861 cggaccatga tgtcgtggct gctaccaaag ttttgcagca ttggctcttc aaaaccggct
    16921 ttttgcaatg catcacgcat tacgtcaatt tcagccggtt tttcgagcgt aatttcgata
    16981 accgtaccac cggtgaaatc cagcccccag ttaaagccgc gcacgcccat aataacgata
    17041 gcggcgatta acagcagacc agagatgccg aaagcccagt agtcccagcg cataaagtca
    17101 tagactttac ggccgtggtt tagttgttca acagtatatt cctgtgccac atcgcactcc
    17161 tcagattgac agcttcttga cgcgcttgcc gccatatagc aggtttacga tggcacgcgt
    17221 accgacaatc gcggtaaaca tcgacgtcgc cacaccgata ccggtagtaa tcgcgaaccc
    17281 tttaattgcc ccggtaccca ctgcgtacag gatgatgact ttaatcagcg tggtgatgtt
    17341 cgcatcgaag atagaactga atgcgccacg ataaccttca tcaattgcct gttgaacagt
    17401 acgtccgttg ctcaactctt ctttaatacg ttcgttgatc agtacgttcg catcgaccgc
    17461 caccgcaagg gttaagacga tacccgcgat acctggcata ctcagcgttg cgcctggcaa
    17521 cagcgacata atgccgacga ttaagatcaa gttggcaatc agagcactgg tcgcaatcag
    17581 accaaacttc ttatagaaga tgatcatgaa cagaatagaa accagcagac cggcaaggca
    17641 agcctccagc ccctgttcaa tgttctgcat acccagggtt gggccaatgg tacgttcttc
    17701 aacaatctga atcggcgcga tcaacgcacc ggcacgcagc agcagtgaca gctgacgggc
    17761 ttcgttcggg ttgttgatgc cggtgatacg gaagctgtta cccagacgag actggatgtt
    17821 ggcgatgtta atcacctctt cctgtttcac cagaaccgca cgaccatttg catctttctt
    17881 accgctgtct ttgtactcca caaacagggt tgccatcggt ttgccgatgt tgtccttagt
    17941 gaagttagac atgatgttac caccagcgct atcgagcgag atgttaacct gcggttggtt
    18001 gtattcgtcc tggctggaag tggagtcggt gatatggtca ccggtcagaa ttacgcgttt
    18061 gtacagcaca actggctgac cttcgcgggt ctgtttcact tcagagtcgc ccggtacgcg
    18121 accggatgcc gcagcggcct ggtcaacgtt ggtgtttacc agacggaatt ccagcgttgc
    18181 cgtcgcaccc agaatctctt tcgcacgcgc agtgtcctga atacctggca gttcaacaac
    18241 gatacggtca gcaccctgac gctgaaccac cggctccgcc acgccaagtt ggtttacacg
    18301 gttacgcagg atattgatgt tctgctgcac cgcatattca cgcgcttcac tcagacgagc
    18361 atcgctcatt accgcacgca gctggttgct gccctggcta ctaatcacca ggtccggatg
    18421 gcgcttgctc agatacgcaa tggcttcatc acgagcttta gcatcgcgga aagtaatgct
    18481 caggccgtag ttgttttctt tacgaacggt ggtatacggg atgccctttt cacgcagatc
    18541 gctgcgcagg ctatcgatat tttgttcctg gagtttgcca agcgcggtgt ccatatccac
    18601 ttccatcagg aagtgaacgc cgccacgcag gtcaaggccg agcttcatcg gctcagcgtg
    18661 aatagctgcc agccagcgcg gcgttgccgg ggcaaggtta agcgccacga cgtatttgtc
    18721 acccataacg cccattaatg cttcacgagc gcgcagctgg gtgtcagtgg agtcgaagcg
    18781 cgcaagaata gcgccctctt ccagtgccac agacttagca gttatttttt cttcttgtaa
    18841 cgttttctgg acctggatca gcgtttgctc actggcggcg actccgcgcg caccagtgat
    18901 ctgaacagcc ggatcctcac caaacaggtt gggaagcgca tacagcagac cgatgacaat
    18961 caccacgatc agcatgacgt acttccacaa aggataacgg tttaacacgg caattccctt
    19021 agggaaaaat tttaattaca gcgccttcat ggtgcctttc ggcaggacgg cagctacgaa
    19081 gtcacgttta ataactactt cagtggtgtc attcagcgcg atagcaatgt agccgttttc
    19141 cgctactttg gttacgcgac caaccaggcc accgttcgtc agaacttcat cacctttggc
    19201 aatggagtcc atcagctttt tgtgttcttt ggtgcgcttc tgctgtggac gcaggatcat
    19261 gaaatagaaa atcagaccga ataccaccag catcaaaatc aaagacatcg ggctaccttg
    19321 cgccggtgca cccgttgccg ctaccgcatc agaaataaaa aagctcatta aatttccctc
    19381 attattaata ttaatcaacg ttcaaaggtg gtacttctcg cccctgacgc tggtaaaaat
    19441 cagttacgaa gctctctaat ttaccctctt caatagcctt gcgtaaaccc gccatcaaac
    19501 gctggtagta acgaaggtta tgaatggtgt tgagtcgcgc gcctaatatt tcgttgcaac
    19561 ggtcaagatg atgcaagtaa gcgcgtgaat aattgcgaca ggtgtagcaa tcacactcag
    19621 gatcgagtgg gccagtatcg ctcttatact tcgcattgcg gattttcacc acgccatcgg
    19681 tcacgaacaa atgaccattt cgggcgttgc gggttggcat tacgcagtca aacatatcga
    19741 taccacgacg tacgccttca accaggtctt ctggtttacc aacgcccatc aggtaacgcg
    19801 gtttgtctgc cggaatttgc gggcatacat gctccagaat gcggtgcata tctgctttcg
    19861 gctcacccac agccagaccg ccgacagcgt agccatcaaa accgatatct accagacctt
    19921 taacagaaat atcacgtaaa tcttcgtaaa cgctgccctg aatgatacca aacagcgcat
    19981 ttttgtttcc gagactgtca aaacgctcac ggctacgctt cgcccaacgc agagacatct
    20041 ccatggagcg ttttgcgtaa tcccagtcag caggatacgg cgtacactca tcaaagatca
    20101 tgacgatatc cgaaccaaga tcgtactgaa tctccattga tttttcagga tcgaggaaaa
    20161 tcggatcgcc gttgatcggg ttacggaagt gcacgccctg ttcggtgatt ttacgaatat
    20221 cgccaaggct gaagacctgg aaaccgccgg agtcggtgag gatcggcccc ttccactgca
    20281 taaaatcgtg cagatcgccg tgcagtttca tgatttcctg gcccgggcgc agccacaggt
    20341 ggaaggtgtt gccgaggata atttgcgcgc cagtggcttc aacttcttcc ggcgtcatcc
    20401 cttttacggt gccgtaggtg ccaacaggca taaaacaagg cgtttccact acgccacgat
    20461 caaagaccag gcggccacgg cgtgcgcgac cgtcggtggt gtccagttca aatttcattt
    20521 tttctccact acgtcagaaa aacagtccaa cgttttaaac cagcgccgcg gaattactcc
    20581 ccgacgcgct cattaattgc ctgcggattg tacgtgataa acatcgcatc accgtaacta
    20641 aaaaagcgat atttctcttc taccgctgct ttataggcgt tcatggtgtg ttgataaccg
    20701 gcaaaggccg aaaccagcat aatcagcgtc gactctggca agtggaagtt cgtcaccagc
    20761 gcatcgacca ctttgtactg gaagcccgga tagataaaga tttgtgtatc gtcgaagaac
    20821 ggttcaatga gatcgttttt cgctgcctga gccgcgcttt ccagcgaacg tactgaagtg
    20881 gtgccaaccg caatcacccg gttaccgcgc gctttcgccg ccagtaccgc gtctaccaca
    20941 tcctgcggta cttcagcgta ttccgagtgc atgatgtgat cttcaatggt gtcgacgcgc
    21001 accggctgga aggtgcccgc accaacgtgc aacgtcacaa acgccatctc cacgcctttg
    21061 gcgcgcaatt tttccagcaa aggctcgtca aaatgcagac cagcggtcgg ggctgcaacc
    21121 gcgcccggtt tttcgctata aacggtttga taaagttcgc ggtcagcgtc ttcgtccgga
    21181 cggtcgatat acggcggcag cggcatatgg ccgatactgt tgaggatatc cagtaccgaa
    21241 cgatcatcat tgaattcgac ttcaaacagt gcgccgtggc gcgcggtcat tgttgcgtta
    21301 atactttcgt catcgcccag cagcagttct gcgccaggtt ttggcgcttt cgaggcgcga
    21361 atatgcgcaa gaatgcgttt gtcgtcgagc atccgctcaa ccagcacttc aatcttgccg
    21421 ccgctggctt tacgcccaaa caggcgcgcc gggatcacgc gggtattatt aaaaaccaga
    21481 agatcgccgg ggttgagctt atcaagtaaa tcggtgaaag taccgtgcgt cagcgcgccc
    21541 gtcggcccgt ccagcgacag taaacgacag ctactgcgtt caggcatggg atagtgggca
    21601 atcagggatt cgggcaattc aaaggagaaa tcggtaacgc gcatgacact gactcagact
    21661 aaaataagag gcgggtagtc tagtgccggg gcgctctccc tgcaaccttt accccctttt
    21721 ggatagaata taatcgatga attttttagc tcacctgcat ttagcccatc tcgcggaaag
    21781 ctcgctttcc ggcaatttac tggctgattt cgtacgcgga aatcctgaag aaagttttcc
    21841 gcccgacgtc gtggctggca ttcatatgca tcgacgtatc gacgtattaa ccgacaatct
    21901 gccggaagtc cgcgaagcac gggaatggtt tcgtagtgaa actcgccgcg ttgcgcctat
    21961 tacgctggat gtcatgtggg atcactttct ttcccgccac tggtcgcagc tgtcgccgga
    22021 ctttccgcta caggaatttg tctgttatgc ccgcgagcaa gtgatgacga ttttgccgga
    22081 ctcaccgcca cgttttatca atctgaacaa ttacttgtgg tcagagcagt ggctggtgcg
    22141 ctatcgcgat atggatttca tccagaacgt gttaaacggc atggcaagcc gccgcccacg
    22201 tctggatgcc ctgcgtgact cctggtacga tttagacgct cattatgacg ccctcgaaac
    22261 ccgcttctgg cagttttatc cgcggatgat ggcgcaggcg tcacacaagg cgttataaaa
    22321 cgttagttca tccataccgt agccgaaatg gcaggcaaag ccagaatccc gtcagtcagt
    22381 tgcccatgcc cttctttgca ttgccattgc acggcattga gcaacggtga cgcgggtagc
    22441 acgacttcac aagcttcgcc acggttgatt gccaccagta cacgctgctg attcagcacg
    22501 cggacaaata ccaccacgtt atcttccgca tacagcacct gacagccgcc atgacgtagc
    22561 gcctgacttt tcttacgcag cgcaatcatt cgctggtaca gcgcgaataa cgccgtatcc
    22621 tgcttttcca cctgccaagg gaacggttta cggcaaaacg gatcgttttt gccatccagt
    22681 cctacttcat caccgtaata aatgcacggt acaccaggcc aggtgaacag ccagaccacc
    22741 gccagcggca ggcgcgcaat atcccgaccg agcagcgttt taaatcgcgc agtatcgtgg
    22801 ctgtcgagct gattaaacat acgtaattgt tgttgatgag aaagccctgc gcggtaatta
    22861 tccatccagg ccatacaggt ttgggcatca atttgctgcg gatcgtaaga gatatcggta
    22921 ttggcaagaa atccccacaa cgggaatgtg aagccacgat agttcatggc ggcatcttcc
    22981 acatcggcct gtaaccattg ccgtgcatcg ccaaaatgtt cgccgacaat atacgcttcc
    23041 ggctgggttt ctttcgccgc ttcggtgatc ccagcaacgt gctgcatatt attgcgcgcc
    23101 ccacccgcct cccccagcat atgcaccaca tccagccgcc agccgtccat actccacggc
    23161 gctttcagcc aatggcggac aatactgtct tccccgcgat aaatttcatt caccagactt
    23221 tccgactgat aatccagctt cggcaagctg gcatagccaa gccagtcgag cgccgtgcca
    23281 tcatcactaa acgagtacca gtcgcgccag ggcgattcag ggttgtgaca agctcccccc
    23341 gtgccacgat tgtgcctgtc aaaccaggca tgggaatcgc cactgtggtt aaacacgcca
    23401 tccagcacca gccgcattcc cagctgctgc gtattgtggc gtaagcgcag caacgcccca
    23461 tcaccgccaa actgcggatc gacatggcga taatcctcgg tatcgtattt atgtacgctg
    23521 ggagctttaa acaccggatt gagatacagc gccgtcacgc caagcttttt cagatacggc
    23581 agtttttcgc ttatcccgtc cagatcgccg ccatagaacg ttgatccgcc cgcctgcgcc
    23641 gtgaccggtt catcccagtc acgcaagatg atctcttgtc cggctgcatg atggtaatag
    23701 acatgatcct gttcagcttc acgaggaaga ctacgcgcaa aacgatcagg gaagatctga
    23761 taaaaaatct gatccgcagc ccattgtggg ccgatatccg gtacatcgac ggcaaactgc
    23821 tccagtcgtg ccggcggcat tcggctgaag ccctgcggtg taaaccaacg ctggcgatcg
    23881 tgccacagca gtttgaaact gtaacgccgc cggggttgtc cgctggagag atcaatcgcc
    23941 gcacgccatg cggtgacgcc aggctgctgc tgactgcgct gcttatgcat cgatactgac
    24001 atttcttcgt tatcgtgttc tgtacgcagc ataatgcgct gcggtgggtc ttcgcccgtc
    24061 agccacagtg taatgagcag ttgatctttg ctttgtttaa caaatggggg caccggcagg
    24121 tgccatgcat ttaacatcat aattcccctg tgatgaaaat ctgatcacct tgccatagcc
    24181 taatgcagga tcactcatca tctgaaggat atttggggga ggaggacggg ggcggataac
    24241 tttcggaata gccggatcag gatttcctcc tgaaccggct tacagaacgc aagtattatt
    24301 ggttttcagc cagctgacga tcgtggcggc gtttaaacat ccagccaatc aacagcacaa
    24361 caatccacgc gaaaccgaca tacagcgaga tacgcgtatc cgggtgataa ccaatcaacc
    24421 cgataataaa gagcaggaaa atcagcccgc cgatggtcgt tgctacccca cccggcactt
    24481 taaatttcag cgccttaact tcttctggcg gcaaacggcg acggaaggca atttgcgaca
    24541 gcaggatcat aatccacacc cacaccgtgg cgaaggttgc cagcgaagcg atcaccagga
    24601 agacgttttc cggcatgatg tagttcagat acaccgcaaa cagcagcgcg gtagtcatca
    24661 ccagcaccgt aacccacgga ataccgcgac gcgacgtttt gctgaaaatt ttcggcgcgc
    24721 tgccctgctc tgccataccg tggagcatac ggcctacgcc aaatacgtca ctgttaattg
    24781 ccgacagcga agcagtcagc acaacaaagt taagaatgct ggcggcaaag gtaatgccca
    24841 tatgctggaa cgtcagcacg aacgggctac cggcagtgcc aacctgattc cacgggtaga
    24901 tagacataat gacgaacagc gtaccgacgt agaacaccag aatacgcatc ggcacggagt
    24961 taatcgcacg cggtatcgat ttctcaggat ctttcgcttc accggcggta atcccgataa
    25021 tttcgatccc accgtaagca aacatcacca tttgcaacga catcaccatc ccaagccagc
    25081 cgttactgaa gaagccgccg ttgctccaca ggttatgaat accggtcggt tgcccgccgt
    25141 tgccaatccc ccagatgatg atgccgaaac cggcgacaat catgatgatg atggtggcga
    25201 ctttaaagaa cgagaaccag aattccagct caccgaagac cttcacgctc atcaggttta
    25261 cggcgcaaat gatcagcacc acgctcagta cccaaatcca gtgcggcacc gtcgggaacc
    25321 agacacccat atagatacca aaagcggtca catcggcgat ggcgacaata aggatttcaa
    25381 agcaataggt ccagccggta atgtaacctg ccagcgggcc gaggttttcc tgcgcataac
    25441 gcgagaaaga gctggctgcc gggttatgta ccgacatttc ccccagcgca cgcataatga
    25501 tatacgccgc gataccaccg ataatatagg ccaacaacac gctcggaccg gccattttga
    25561 tggcgtctgc cgaaccgtaa aacagcccgg tgccaattgc tgaacccagt gccataaagc
    25621 gtatgtggcg ggtacttagc ccacgcttta gcttgttctt actttccatc aattcctaac
    25681 ccatcaacac aataaaaaac cacggggcat cgcagccccg tggttaaaac aaatgttcag
    25741 tgatttagtg agcgctggag gtcacctgac gacctgccgc gcgatcccag ataatggcca
    25801 gaaccaccat caccactgtt ggcattaacc acgccagacc ttgttcggcc agcggtaaac
    25861 gctgcgccca ggacggtaag atatcgctga atgcagatgc tttgatcccg tcgagaatac
    25921 caaaaagcag gctgataaac atcggcggag caatcacgcg ggacgaatta tgccaccatg
    25981 agcgtgtaaa acttaataca accagtgcga tacacggcgg ataaatagcg gtcagcaccg
    26041 gtacggagat ctggatcagc tggcttaagc cgaggttaga aaccaccatc gagaagccgc
    26101 cgaggataaa caccagcgta cgataagaga gcggtacgta ctgggcaaag aattctgcac
    26161 aagcacaggt caggccaact gccgttacca ggcaggcgat gaagattaac gccgccagca
    26221 ggaagctacc gccgccgcca aaggtgtgct gaacgtaagc atgcagaata gcagcgccgt
    26281 ttgcagactg atcgaccagc gacgcgctgt ctgaacccag acggaacagc gccaggtaca
    26341 gcagagtcag accaacaccc gccatcaggc cagcccagac ggtataacgg gtcagcagac
    26401 gcgcttcggt aacgccacga gaacgcgccg cgttaacaat aacgatacca aacaccattg
    26461 cgcccagcgt atccatggtc agatagccgt taacgaagcc gttagaaaac gcagcgtttt
    26521 gataagcctc agtcgccgtg ctgatagaac ccgccggcca gacaatcgcg gcaacagaca
    26581 ggatgaccag cgcgataatt ttcagcggcg caaggaagtt gcccacggta tccagcagct
    26641 tgcccggata gagcgaaacc agaataacga tagcgaaata gaccaggctg tagataaaca
    26701 gcggcagcgc ggaatcacca gtcagcggcg caatccccac ttcaaaggaa acggtagctg
    26761 tacgcggcgt agcgaaaagc ggccccaccg ccaggtaaca aaccgttgcc agcagtacgc
    26821 cagcgacttt accgattggc gtgctgaggc tgtcaacacc gccgccaact tttgccagcg
    26881 ccactaccgt tagtaccggc agaccaacgg cagtaatgag gaagccgaat gccgcagtcc
    26941 agacgtgttc gcctgcctgc aagccgacca ttggagggaa aataatgtta cctgcgccga
    27001 cgaacaacgc aaatgtcata aagcccagag cgatgatatc gcgcgatctt aattgatggg
    27061 tcataaatca tactgcctgt ggatgtggtg ttgtaacgtt aaatttttgt gccaatccct
    27121 ggggatgata cagcggtata tttaaacaat tttagcggga aatactccca accaggcgcg
    27181 gcgaagaata gtttttcgat ttaccgcgtt aatgcagtca gtatgacagc acctgaagcg
    27241 caatttaaac gcttataacg ttttaaagca aggagggatc taaaaaccag aggtatatag
    27301 cgataagaaa tcaccaacca gtataaaaca ctatttccgt gaaaaagaca tggctaatca
    27361 tgcgaacaaa aaatcaccaa ccgttgtaaa atctaacgcc cgtattttga ctgcaaagaa
    27421 aataagccag catcggctgg cttatggcaa taaaagatga caaaggcgga ttaatcgctg
    27481 tttttggcaa ttaaacgttc cgggataaca aaactgaaac gtgttccttt tcctactgta
    27541 ctctcaatat tcaggcgact ttcgtgatga ttcaccgcat gtttcacgat cgctaaccct
    27601 aatccgctac cgccggtttg ccgggaacgc gctttatcaa cgcgataaaa acgctcggtc
    27661 aggcgcggaa tatgctccgg tgcaatgccc ggtccgttat cttcaacgct aaattcggca
    27721 ccgtgcggca ctcgctgcca gcgtacggtg atatgcgtgc cttctggagt atgattcacg
    27781 gcgttataga ccaggttcga aatcgcactg cgtagctgat cttcgttgcc agacactttg
    27841 aggccgttat ctatctcaaa ggtaaatgtc tgttttttct gactcagagt ctgagcctca
    27901 cgctcaacaa cgcgcagcat catcggcaca tcaacctttt cattgagcaa atgcgtcggt
    27961 gcggcttcta ttttcgacag cgtcagcaac tgcttcacca gcccttccat ccgctgggtc
    28021 tgctcgcgca tggtgtgcaa cgctttttcg cgtaccgcgc cttccagcgg ctgctcatcc
    28081 atcatctcca ggtaaccctg taacacggtc aatggcgtac gtaactcatg gctcacgttg
    28141 gcaaaaaagt tacgccgcgc cccttccagt tgatgcattt gcgtgacatc acgcgccacc
    28201 atcagcaact gtttgtgggt ataaggcatg acgcgaattt ccagatgccg cccggtgttg
    28261 agcaccagat tgagcgggcg agaaaaatca cgcgttttca gatattgcgt aaactccggg
    28321 taacgcagca ggttaaggat gttctgcccg ttatcttccg gccagcgcaa accaagaatt
    28381 tgttgcgcca gaccgttaca ccagaaaata ccgccctctt cagtggtcag caccactgcg
    28441 tcgggcagcg actccgcgcc gctacgaaag cgtttaatca gattgcccag ttcacggcgg
    28501 cgttttttat ttcgcagctg catctggtgt aagccgtata gtagcggttc ccagctacca
    28561 cgccccggtg gcggggtcat actacgatcc acccacagcc accatgaaag gcgcaataaa
    28621 ttccagaaat gccagataag cagtcccgtt accgatgcca gcaaaaacca gggcaggtaa
    28681 ccaaaaaatg cacccaggat gaaagccggg aagcagcaaa gtagcagctc cagcaccagc
    28741 cttttccacg acagccgttc cagcacgtaa gatactccag ttaagaaatc ataagccctg
    28801 ctctgcgtcc gatgagcaag gcgttaaaag cgggttgaaa aacgatatcc tgtaccgcgc
    28861 acggtctgca ccatgcggtc atgcccgcct ggctccagtg ctttacgtag gcgacgaatg
    28921 tggacatcga ccgtacgatc ttccacatac acgttagttc cccagacgtg gtttaacagc
    28981 tgctcgcggc tgtacacgcg ctcaggatgc gtcataaaaa agtgcagcag tttaaattct
    29041 gtcggcccca tctccagcgg ctcttcgccc gccatcactc ggtgagatgt cgggtcgaga
    29101 cttaatccct gcatctcaat cacctcttcc accgccattg gcgaaatacg gcgcattacc
    29161 gctttgattc gcgccaccag ctccttcggc gaaaacggct tggtgatata gtcatccgcg
    29221 ccggtttcaa ggccgcgcac gcgatcttct tcttcccctc tggcggtcaa catcaccact
    29281 ggaatatccc gggtcatcga ctcgcgcttg aggtgtttga tgaactggat accggagccg
    29341 ccaggtaaca tccagtcaag gagaattaaa tccggccagg gttcattcag ttgattcaca
    29401 gcactgtcat aatcttccgc ttcgaccggc tgaaagccat tttgttcgag cacgaagcag
    29461 accatttcgc gaattggagc ttcatcttct acgaccagaa tacgtctcgc catgatttgc
    29521 cctgttgtaa taaataggtt gcgatcatta atgcgacgtc attatgcgtc agatttatga
    29581 cagatttatg aaaagctcgt cgcacatatc ttcaggttat tgatttccgt ggcgcagaaa
    29641 aaagcaaatg gcacatctgt ttgggtataa tcgcgcccat gctttttcgc cagggaatcg
    29701 ttatgcgcat ccttcacacc tcagactggc atctcggcca gaacttctac agtaaaagcc
    29761 gcgaagctga acatcaggct tttcttgact ggctgctgga aacagcacaa acccatcagg
    29821 tggatgcgat tattgttgct ggtgatgttt tcgataccgg ctcgccgccc agttacgccc
    29881 gcacgttata caaccgtttt gttgtcaatt tacagcaaac tggctgtcat ctggtggtac
    29941 tggcaggaaa ccatgactcg gtcgccacgc tgaatgaatc gcgcgatatc atggcgttcc
    30001 tcaatacgac cgtggtcgcc agcgccggac atgcgccgca aatcttgcct cgtcgcgacg
    30061 ggacgccagg cgcagtgctg tgccccattc cgtttttacg tccgcgtgac attattacca
    30121 gccaggcggg gcttaacggt attgaaaaac agcagcattt actggcagcg attaccgatt
    30181 attaccaaca acactatgcc gatgcctgca aactgcgcgg cgatcagcct ctgcccatca
    30241 tcgccacggg acacttaacg accgtgggtg ccagtaaaag tgacgccgtg cgtgacattt
    30301 atattggcac gctggacgcg tttccagcac aaaactttcc accagccgac tacatcgcgc
    30361 tcgggcatat tcaccgcgca cagattattg gcggcatgga acatgttcgc tattgcggct
    30421 ctcccattcc actgagtttt gatgaatgcg gaaagagtaa atatgtccat ctggtgacat
    30481 tttcaaacgg caaattagag agcgtggaaa acctgaacgt accggtaacg caacccatgg
    30541 cggtgctgaa aggcgatctg gcgtcgatta ccgcacagct ggaacagtgg cgcgatgtat
    30601 cgcaggagcc acctgtctgg ctggatatcg aaatcactac tgatgagtat ctgcatgata
    30661 ttcagcgcaa aatccaggca ttaaccgaat cattgcctgt cgaagtattg ctggtacgtc
    30721 ggagtcgtga acagcgcgag cgtgtgttag ccagccaaca gcgtgaaacc ctcagcgaac
    30781 tcagcgtcga agaggtgttc aatcgccgtc tggcactgga agaactggat gaatcgcagc
    30841 agcaacgtct gcagcatctt ttcaccacga cgttgcatac cctcgccgga gaacacgaag
    30901 catgaaaatt ctcagcctgc gcctgaaaaa cctgaactca ttaaaaggcg aatggaagat
    30961 tgatttcacc cgcgagccgt tcgccagcaa cgggctgttt gctattaccg gcccaacagg
    31021 tgcggggaaa accaccctgc tggacgccat ttgtctggcg ctgtatcacg aaactccgcg
    31081 tctctctaac gtttcacaat cgcaaaatga tctcatgacc cgcgataccg ccgaatgtct
    31141 ggcggaggtg gagtttgaag tgaaaggtga agcgtaccgt gcattctgga gccagaatcg
    31201 ggcgcgtaac caacccgacg gtaatttgca ggtgccacgc gtagagctgg cgcgctgcgc
    31261 cgacggcaaa attctcgccg acaaagtgaa agataagctg gaactgacag cgacgttaac
    31321 cgggctggat tacgggcgct tcacccgttc gatgctgctt tcgcaggggc aatttgctgc
    31381 cttcctgaat gccaaaccca aagaacgcgc ggaattgctc gaggagttaa ccggcactga
    31441 aatctacggg caaatctcgg cgatggtttt tgagcagcac aaatcggccc gcacagagct
    31501 ggagaagctg caagcgcagg ccagcggcgt cgcgttgctc acgccggaac aagtgcaatc
    31561 gctgacagcg agtttgcagg tacttactga cgaagaaaaa cagttactta ccgcgcagca
    31621 gcaagaacaa caatcactaa actggttaac gcgtctggac gaattgcagc aagaagccag
    31681 ccgccgtcag caggccttgc aacaggcgtt agccgaagaa gaaaaagcgc aacctcaact
    31741 ggcggcgctt agtctggcac aaccggcacg aaatcttcgt ccacactggg aacgcatcgc
    31801 agaacacagc gcggcgctgg cgcatattcg ccagcagatt gaagaagtaa atactcgctt
    31861 acagagcaca atggcgcttc gcgcgagcat tcgccaccac gcggcgaagc agtcagcaga
    31921 attacagcag cagcaacaaa gcctgaatac ctggttacag gaacacgacc gcttccgtca
    31981 gtggaacaac gaactggcgg gttggcgtgc gcagttctcc caacaaacca gcgatcgcga
    32041 gcatctgcgg caatggcagc aacagttaac ccatgctgag caaaaactta atgcgcttgc
    32101 ggcgatcacg ttgacgttaa ccgccgatga agttgctacc gccctggcgc aacatgctga
    32161 gcaacgccca ctgcgtcagc gcctggtcgc gctgcatggg cagattgttc cccaacaaaa
    32221 acgtctggcg cagttaatgg tcactatcca gaatgtcact ctggaacaga cgcaacgtaa
    32281 tgccgcactt aacgaaatgc gccagcgtta taaagaaaag acgcagcaac ttgccgatgt
    32341 gaaaaccatt tgcgagcagg aagcgcgcat taaaacgctg gaagcccagc gcgcgcagtt
    32401 acaggcgggt cagccttgcc cactttgtgg ttccaccagc cacccggcgg tcgaggcgta
    32461 tcaggcgctg gagcctggtg ttaatcagtc tcgattactg gcgctggaaa acgaagttaa
    32521 aaagctcggt gaagaaggtg cgacgctacg tgggcaactg gacgccttaa caaagcagct
    32581 tcagcgtgat gaaaacgaag cgcaaagcct ccgacaagat gagcaagcac ttactcaaca
    32641 atggcaagcc gtcacggcca gcctcaatat caccttgcag ccacaggacg atattcaacc
    32701 gtggctagat gcacaagatg agcacgaacg ccagctgcgg ttactcagcc aacgacatga
    32761 attacaaggg cagattgccg cgcataatca gcaaattatc cagtatcaac agcaaattga
    32821 acaacgccag cagcagctct taacggcatt ggcgggttat gcactgacat tgccacagga
    32881 agatgaagaa gagagctggt tggcgacacg tcagcaagaa gcgcagagct ggcagcaacg
    32941 ccagaacgaa ttaaccgcgc tgcaaaaccg tattcagcag ctgacgccga ttctggaaac
    33001 gttgccgcaa agtgatgagc tcccgcacag cgaagaaact gtggcgctgg aaaactggcg
    33061 gcaggttcat gaacaatgtc tcgcattaca cagccagcag cagacgttac agcaacagga
    33121 tgttctggcg gcgcaaagtc tgcaaaaagc ccaggcgcag tttgacaccg cgctacaggc
    33181 cagcgtcttt gacgatcagc aggcgttcct tgcggcgcta atggatgaac aaacactaac
    33241 gcagctggaa cagctcaagc agaatctgga aaaccagcgc cgtcaggcgc aaacgctggt
    33301 cactcagaca gcagaaacgc tggcacagca tcaacaacac cgacctgacg ggttggctct
    33361 cactgtgacg gtggagcaga ttcagcaaga gttagcgcaa actcaccaaa agttgcgtga
    33421 aaacaccacg agtcaaggcg agattcgcca gcagctgaag caggatgcag ataaccgtca
    33481 gcaacaacaa accttaatgc agcaaattgc tcaaatgacg cagcaggttg aggactgggg
    33541 atatctgaat tcgctaatag gttccaaaga gggcgataaa ttccgcaagt ttgcccaggg
    33601 gctgacgctg gataatttag tccatctcgc taatcagcaa cttacccggc tgcacgggcg
    33661 ctatctgtta cagcgcaaag ccagcgaggc gctggaagtc gaggttgttg atacctggca
    33721 ggcagatgcg gtacgcgata cccgtaccct ttccggcggc gaaagtttcc tcgttagtct
    33781 ggcgctggcg ctggcgcttt cggatctggt cagccataaa acacgtattg actcgctgtt
    33841 ccttgatgaa ggttttggca cgctggatag cgaaacgctg gataccgccc ttgatgcgct
    33901 ggatgccctg aacgccagtg gcaaaaccat cggtgtgatt agccacgtag aagcgatgaa
    33961 agagcgtatt ccggtgcaga tcaaagtgaa aaagatcaac ggcctgggct acagcaaact
    34021 ggaaagtacg tttgcagtga aataactatt cagcaggata atgaatacag aggggcgaat
    34081 tatctcttgg ccttgctggt cgttatcctg caagctatca ctttattggc tacggtgatt
    34141 ggtagccgtt ctggtggttg tgatggtggt atgaaaaaag tcattttatc tttggctctg
    34201 ggcacgtttg gtttggggat ggccgaattt ggcattatgg gcgtgctcac ggagctggcg
    34261 cataacgtag gaatttcgat tcctgctgcc gggcatatga tctcgtatta tgcactgggg
    34321 gtggtggtcg gtgcgccaat catcgcactc ttttccagcc gctactcact caaacatatc
    34381 ttgttgtttc tggtggcgtt gtgcgtcatt ggcaacgcca tgttcacgct ctcttcgtct
    34441 tacctgatgc tcgccattgg tcggctggta tccggctttc cgcatggcgc attttttggc
    34501 gtcggagcga tcgtgttatc aaaaattatc aaacccggaa aagtcaccgc cgccgtggcg
    34561 gggatggttt ccgggatgac agtcgccaat ttgctgggca ttccgctggg aacgtattta
    34621 agtcaggaat ttagctggcg ttacaccttt ttattgatcg ctgtttttaa tattgcggtg
    34681 atggcatcgg tctatttttg ggtgccggat attcgcgacg aggcgaaagg aaagctgcgc
    34741 gaacaatttc actttttgcg cagcccggcc ccgtggttaa ttttcgccgc caccatgttt
    34801 ggcaacgcag gtgtgtttgc ctggttcagc tacgtaaagc catacatgat gtttatttcc
    34861 ggtttttcgg aaacggcgat gacctttatt atgatgttag ttgggctagg gatggtgctg
    34921 ggaaatatgc taagtggcag gatttcagga cgttattcac cactgcgcat tgcagcagtg
    34981 actgacttta taattgtact ggcactgctg atgctctttt tctgcggcgg catgaaaaca
    35041 acgtcgctta tttttgcttt tatttgttgc gcgggattat ttgccctttc agcaccgcta
    35101 caaatattgt tactacaaaa cgccaaaggc ggagagttat taggtgccgc aggtgggcaa
    35161 atagcgttta acctcggtag cgccgtcggc gcatattgcg gtggtatgat gctgacgctg
    35221 gggctggcat ataattacgt ggcgctgcct gccgccctgc tttcgtttgc tgcgatgtcg
    35281 tcgttgctgc tgtatggtcg ctataagcgc cagcaagcgg cggatactcc ggtgctggcg
    35341 aaaccactgg ggtaggttat agtctcggtg ctcattactt attgccggat gcggcgtgaa
    35401 cgccttatcc gccctacgcg gttctggcac attttgcagg cctgataaga cgcggcaagc
    35461 gtcgcatcag gcatgatgcg ccaattgcct acgtttttta ctcttgtggc cataaccacg
    35521 cagcgccgcg tacgccgctg gaatcaccgt gcttcgcctt acgcaccggc gtttcacatt
    35581 cgccgccgaa gacaaattgt ttaatcaact gcccaaccgt ttgatataaa cggtctacat
    35641 tgctcatccc gccccccagg acaatcacat ccggatcgag aatattcacg acatgtgcca
    35701 gcgattttgc cagccgcagc tcgtagcgac gcaatgccag ttccgctacc ggatcgcttt
    35761 cttcaaccag gctgataatt tcactgcctt tcagcgcatg tccgctcaaa cgacgataat
    35821 ctgtggcaaa tcccgtgccc gaaataaagg tttcaataca accttgttta ccgcaataac
    35881 aagggacttc ctcgcgataa cgcagttcgt cttcgtccat ccacggtagc ggattgtgtc
    35941 cccactcacc tgccgtgcca ttgccgccga tatgcgcccg cccattgaat gccacgcccg
    36001 cgccgcatcc cgtgccgata atcacggcaa atactgtctg cgctcccgct gccgcgccat
    36061 ctactgcttc tgaaaccgcc agacagttgg cgtcatttgc cagccgcact tcccgctgca
    36121 acctcgcgct taagtcttta tcgaatggct gaccgttgag ccaggttgaa ttggcattct
    36181 taaccacacc ggtgtaaggc gaaattgagc caggaatgcc catacctacc gttccgcgct
    36241 gccctgtcgc ctgctccgcc atatcaacca acgtggcgat cgtttcaata gtctgccggt
    36301 aatcatcacg cggcgtgggc agacgatggc ggtacaactg ctcccctgca tcgcccagtg
    36361 caatcacttc agttttggtg ccgcctaaat cgatacctat acgcacggta ctctccttat
    36421 ttttttcaat atcaatagcg tagagacgga caaccggatt ggcaatgcaa ggccgccgac
    36481 aattcgttat catgcccgct aaatttaacg acaaggccgt ggaaattatc atgctgtggt
    36541 tcaaaaattt aatggtttac cgtcttagcc gcgagatttc gctgcgtgca gaagagatgg
    36601 aaaaacagct agcctcgatg gcatttaccc catgcggcag ccaggacatg gcgaagatgg
    36661 gctgggttcc tccgatggga tcgcacagcg atgcgttaac gcacgttgcc aatggtcaaa
    36721 ttgttatctg cgcacgcaaa gaagaaaaaa tcctcccgtc tccggtgatt aaacaggcgc
    36781 tggaagcgaa aatcgccaag ctggaagcgg aacaggcgcg taagctgaag aaaaccgaaa
    36841 aagattcgct gaaagacgaa gtgctgcact ctctgctgcc gcgagctttc agccgtttta
    36901 gccagacaat gatgtggatc gacacggtta atggtttgat tatggtggac tgcgccagcg
    36961 ccaaaaaagc ggaagatacg ctggcattac tgcgtaaaag cctggggtcg ttacctgttg
    37021 taccattgag catggaaaac ccgattgaac tgacgctaac cgaatgggtt cgctccggta
    37081 gcgcagcaca gggcttccag ttgcttgatg aagccgagct gaaatcgttg ctggaagatg
    37141 gcggcgtgat ccgcgcgaag aaacaagatc tgaccagcga agagatcacc aatcacatcg
    37201 aagccggaaa agtggtgact aaactggcgc tcgactggca gcagcgcatt cagtttgtga
    37261 tgtgcgacga tggttcgctc aagcgtctga agttctgcga cgagctgcgc gatcaaaacg
    37321 aagatatcga ccgtgaagat ttcgcccagc gttttgatgc cgatttcatc ctgatgactg
    37381 gtgaactggc agcgttaatt caaaacctga ttgaaggatt aggtggcgaa gcacaacgtt
    37441 aattgctgat tttcctttaa tgccggatgc gacgcctgcc gcgtcttatc cggcgtacga
    37501 agccacacca ggcatataat tattcgctac ggcgggcaat aatttttagc gcagcaatat
    37561 tatgcgtttt atgctgtaac ttgctccatg gacgttgtgt cattgttttt cctcaagcca
    37621 gtgacgcgct tcactgcgaa ttttccgcat tgccccggtt ttcactaaat cccgtaacag
    37681 cgaaagatcg ggttgttcga caaataagag taatgatgga tcctctttta accacgtcgt
    37741 tttcacatcg gcagcgagct gtggattatt catagctagc gaccgatgcc gagactcggt
    37801 caatgtcgtt aacaacgacg ccggggtatg tgcgttgcgt aatgccgttt ggcgcaaatg
    37861 atcattgcga ctttgcagca atgcacgcca ccattcatca tctgatttat cttttatcgt
    37921 agccagttcc gcaacaatat ctggagatct tcactgatat ttatatcaac cttgatgaat
    37981 tatgaatata tcggaaattg aaaaaatcgc agcaaaggaa atattattat gggttaaaga
    38041 gaggatattt cagaaaaatg ccctcccgtt caaggggaag gcgaggaatt acagatagcg
    38101 gcacagataa gaggtgggtt cggcaacttg cagatgaaac tcactgtgac tgggaacatt
    38161 aaacaccgaa ccggcttcat acacctgcca gtcggtcgcg tcaggcagta acacattcag
    38221 cgcgccactg attaccgtca tctcttccgg ctcagcggtg ctgaaggtgt attcgccttc
    38281 aaccataaca cccacgctgg cgcgaccagt gctgctgctg gaaaagccga ttgatttcac
    38341 tttgccggaa aagtactcat tactttgaag cataaactgg ccctgtctgg tagttgaaca
    38401 tctacttcac tataggggcc agaggcgctg gctgtcacgc aaaattacac cagtaattcc
    38461 gcagccagcc gtgcaatcaa tacgttagac agcaagacag gaacatcgag ctgtttttgc
    38521 agtaaatcgc gatgacgttg gtgaaatcct aaacaatcca gcatgatgac atcagcccct
    38581 tttgccagta attctttccc ggcatcaatg attttttgtt ctgaatcatg aatggggtta
    38641 cccaatgaaa ataccggcga tttctgcaaa atttgccatt tttgcgcctg aacggtcagc
    38701 atctcctcaa ccggaacgat aacccccacc tgatgatctt caacaataga ggaaaccagt
    38761 ggaggcaata ttcgcgacgg ctcaagaaag atcgtattac gcgcagtcat actactaatg
    38821 tttgctgtac tcattaataa aatgacgtca taaccctgat tatcgagcac ttcaaccaca
    38881 ccttgcaggt ctcgctccac tttacgacgc gaaacatggg ccagctggtt gtcatttaat
    38941 aatgtgagaa tggtgtcttc gcctgcttct ggcgcgtact ccgccatcac ttcttcacga
    39001 cttaacttcc ccagcaggct atgatgggaa atattatctt cgtcaatgta ttccgtcagg
    39061 agcggcaaaa cctcctgcat aggtacaatg ccgatggtca ggatcgccaa cgacgcactc
    39121 attgttcatc caccttactt ttctttcgtc gttaattacc gggcaagtgt gaagcaccac
    39181 tgctgacact tacttgtgct acaaatgaca aaaagcgtag cagcagacgt gcacggcata
    39241 atgtaaagat tccaaatgat tccagtaatg gattgttatt gtttaatatt ctaattatta
    39301 gaaaaacatg aattatgaaa aatgtgacgc agatcccttg tagtggcact cagaatccct
    39361 tccgagcagt ctgatgcggt cactctttgt cagggtcctc atagcgtttt ttcgcatcca
    39421 tcgcttcctg agccgtcgga tgttcactga tcaacgagtc tggtttagga tgatcggctc
    39481 tgagttggta ccaggttacc gtctgtcctg gctttccttt ttcaatcgtc actatatatg
    39541 cttcacgagg ataaggcggt ttcgttggca tcgttcttcc ttatttcacg ggatgaacgt
    39601 taagtatagg cgctctaaaa tcaacaattg atcgtctgtg ccagggcgct acgaatttca
    39661 gaaatcacct ggctgggttc gtttgttgcg tcgatgataa tatgcgcaac ttcgcgatat
    39721 agcgcatcgc gttcttccag cacttcctga acttcttcgc tcagcggttt tcccgttaag
    39781 gttggccgta aatcttcttc cggtgcagct tgcagtcggt taaccaggac tgatactggc
    39841 gcacacaaat aaaccacgat cccgttattt tgcatgaagt gacgattaaa ttccgtcaga
    39901 ataatgccgc cgcctgtagc gataacggtg gatggcgcag ttaccgcttc cagcgccgcc
    39961 gtttctctgg cgcgaaatcc cgcccactct tccctttcga cgatctccgc gaccgtcata
    40021 ttgagctgtg attgcaacca ctgatcggta tcgacaaacc gacggttaag cgaatcggca
    40081 agggccattc cgaccgttgt tttaccacag ccccgaggcc cgatcagaaa aagaggttgt
    40141 gtcatcgtgg gttttcccca ataggtcgca atgcggcgaa agccggtgtc atgagaatag
    40201 cgatcatacc atcaaactag tacaatttcg attgtaaaga aaaaattcca cttaaagtga
    40261 a
//
LOCUS       DAPHDW010000028        38562 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.028, whole genome shotgun sequence.
ACCESSION   DAPHDW010000028 DAPHDW010000000
VERSION     DAPHDW010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38562)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38562)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38562
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.028"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            300..1616
                     /gene="gntT"
                     /locus_tag="RH057_001443"
     CDS             300..1616
                     /gene="gntT"
                     /locus_tag="RH057_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV3127838.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(1661..3745)
                     /gene="malQ"
                     /locus_tag="RH057_001444"
     CDS             complement(1661..3745)
                     /gene="malQ"
                     /locus_tag="RH057_001444"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HDV3127839.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWLLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMTAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYQNVDSPEVRQFC
                     EEHRNDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLETMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(3755..6148)
                     /gene="malP"
                     /locus_tag="RH057_001445"
     CDS             complement(3755..6148)
                     /gene="malP"
                     /locus_tag="RH057_001445"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HDV3127840.1"
                     /translation="MSQPIFNDKQFQEALSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVTKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLIDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VRLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENPQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            6760..9465
                     /gene="malT"
                     /locus_tag="RH057_001446"
     CDS             6760..9465
                     /gene="malT"
                     /locus_tag="RH057_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
                     /protein_id="HDV3127841.1"
                     /translation="MLIPSKLSRPVRLDHTVVRERLLAKLSGANNFRLALITSPAGYG
                     KTTLISQWAAGKNDIGWYSLDEGDNQQERFASYLIAAVQQATNGHCAICETMAQKRQY
                     ASLTSLFAQLFIELAEWHSPLYLVIDDYHLITNPVIHESMRFFIRHQPENLTLVVLSR
                     NLPQLGIANLRVRDQLLEIGSQQLAFTHQEAKQFFDCRLSSPIEAAESSRICDDVSGW
                     ATALQLIALSARQNTHSAHKSARRLAGINASHLSDYLVDEVLDNVDLATRHFLLKSAI
                     LRSMNDALITRVTGEENGQMRLEEIERQGLFLQRMDDTGEWFCYHPLFGNFLRQRCQW
                     ELAAELPEIHRAAAESWMAQGFPSEAIHHALAAGDALMLRDILLNHAWSLFNHSELSL
                     LEESLKALPWDSLLENPQLVLLQAWLMQSQHRYGEVNTLLARAEHEIKDIREGTMHAE
                     FNALRAQVAINDGNPDEAERLAKLALEELPPGWFYSRIVATSVLGEVLHCKGELTRSL
                     ALMQQTEQMARQHDVWHYALWSLIQQSEILFAQGFLQTAWETQEKAFQLINEQHLEQL
                     PMHEFLVRIRAQLLWAWARLDEAEASARSGIEVLSSYQPQQQLQCLAMLIQCSLARGD
                     LDNARSQLNRLENLLGNGKYHSDWISNANKVRVIYWQMTGDKAAAANWLRHTAKPEFA
                     NNHFLQGQWRNIARAQILLGEFEPAEIVLEELNENARSLRLMSDLNRNLLLLNQLYWQ
                     AGRKSDAQRVLLDALKLANRTGFISHFVIEGEAMAQQLRQLIQLNTLPELEQHRAQRI
                     LREINQHHRHKFAHFDENFVERLLNHPEVPELIRTSPLTQREWQVLGLIYSGYSNEQI
                     AGELEVAATTIKTHIRNLYQKLGVAHRQAAVQHAQKLLKMMGYGV"
     gene            complement(9508..10524)
                     /gene="rtcA"
                     /locus_tag="RH057_001447"
     CDS             complement(9508..10524)
                     /gene="rtcA"
                     /locus_tag="RH057_001447"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HDV3127842.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITSIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGEYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFNTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGSFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFKVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(10528..11754)
                     /gene="rtcB"
                     /locus_tag="RH057_001448"
     CDS             complement(10528..11754)
                     /gene="rtcB"
                     /locus_tag="RH057_001448"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HDV3127843.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAEDLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPVNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESDQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLKAVAWAQLFASLNRDAMMENVVTALQSVTQKTVRQQQTLAMEE
                     INCHHNYVQKEQHFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            11943..13541
                     /gene="rtcR"
                     /locus_tag="RH057_001449"
     CDS             11943..13541
                     /gene="rtcR"
                     /locus_tag="RH057_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HDV3127844.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPTLCLCQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVSVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSPPRKKEQPRGAGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRLQLEHVIALCRQAKSLSAAGRLLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(13523..14281)
                     /locus_tag="RH057_001450"
     CDS             complement(13523..14281)
                     /locus_tag="RH057_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HDV3127845.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(14298..15128)
                     /gene="glpG"
                     /locus_tag="RH057_001451"
     CDS             complement(14298..15128)
                     /gene="glpG"
                     /locus_tag="RH057_001451"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HDV3127846.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWQAGHTGSGLHYRRYPFFAALRERAGPVTWVVMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYVQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(15173..15499)
                     /gene="glpE"
                     /locus_tag="RH057_001452"
     CDS             complement(15173..15499)
                     /gene="glpE"
                     /locus_tag="RH057_001452"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HDV3127847.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            15689..17194
                     /gene="glpD"
                     /locus_tag="RH057_001453"
     CDS             15689..17194
                     /gene="glpD"
                     /locus_tag="RH057_001453"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HDV3127848.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNTHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYARTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRADDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(17248..17853)
                     /locus_tag="RH057_001454"
     CDS             complement(17248..17853)
                     /locus_tag="RH057_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000589351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127849.1"
                     /translation="MIKFRLYIPPVILGFVIVPLLVWPTVIVLAVLIFTLTFLAEIIF
                     SFPLLVVRISLQELQLELMVEYALFFSVMAGIGWQFSRRTPPELKNRLHCWLVFSPVY
                     FWLILSNFILYISPEKSALLENIRNFFLTFVWLPLNFSPFWPQPWTDFVGPISAQLGF
                     ALGYYCHWCRKNRNQRKKWGDWVTCLSLAILAQGPLFNYLQ"
     gene            complement(17856..18623)
                     /locus_tag="RH057_001455"
     CDS             complement(17856..18623)
                     /locus_tag="RH057_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001182130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127850.1"
                     /translation="MQPSPAMQALIEQIYHIFRRYPVPQKFVVCCEYCLSQQEQKVLR
                     STSLRAISYSLINAWNSSPGPDPQNSDEVRYFLPRLLEFVAQGQFDNIHEVFSLRRIN
                     LASKENWREDEWKILQRFACQYMTDWVSGDEAVELQYMLEMFFRADIALAPLLDAINS
                     VPGFWSTVSLACLLNRYCEDYIRDNQDDIDNVITTQINAWAFNNQSILKERARQAIEN
                     PPKQPEQGTQHQVWVDDWIIDECLCAMYDASSESPGK"
     gene            complement(18626..19336)
                     /locus_tag="RH057_001456"
     CDS             complement(18626..19336)
                     /locus_tag="RH057_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127851.1"
                     /translation="MELVNTLFASLVGTDPFTGVDITIANCKSAYWDEGIVQQLINQA
                     LDEGEKFVGADGLEGLLRYNVTLNIGLTSSNVWPGFSLDTATISRLCACGADFGFDPY
                     ISDVPDVQCDLNTTNDLTVQFTAMLNPDERVIIAKRPLKKCESWIEDIYIFQVFKDAW
                     KFHNDNSLRGFRDKQAELKLYARYYTVENCAEESCRDCNSCIRPSFSLSRSAIIRLNV
                     ANARFVYQPFTRDQRARG"
     gene            complement(19351..20859)
                     /locus_tag="RH057_001457"
     CDS             complement(19351..20859)
                     /locus_tag="RH057_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312299.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV3127852.1"
                     /translation="MMQNRKWILTSLVMTFFGIPILAQFLAAVIAMLGVGLAGIIEVC
                     NIFITPTIYLLLNIFMLALGALMLFFSGRVWADDSAPEKREIAVWRQCLFLVPALLTL
                     GVWIIALHLADYQFRQMGAGWLADLMLPWLGVLLASLVGGEYWWLVIIPVGAHISFSL
                     GYGWPTRYPLTGTSGLRCRNSLLFILLMLGFVAGYQAYLYKQLNPGVGVRENIDTWAW
                     RPDKLNNQLTPLRGKPQIQFTQNWPRLDGATAAYPIYASAFYALSVLPEDFHEWEYLA
                     NSRTPEAYNKIVKGNADIIFVAQPSGGQKKRAEESGVTLIYTPFAREAFVFIVNVDNP
                     VNSLTEQQVRDIFSGAITNWRTVGGNDQEIQTWQRPEDSGSQTVMQSQVMKNVRMISP
                     QETEVASMMEGMIKVVAEYRNTNNAIGYTFRYYATQMNADKNIKLLAINGIAPTAENI
                     RNGKYPYIVDAFMVTRENMTSETQKLVEWFLTPQGQSLVEDVGYVPMYKTLH"
     gene            complement(20984..23431)
                     /gene="glgP"
                     /locus_tag="RH057_001458"
     CDS             complement(20984..23431)
                     /gene="glgP"
                     /locus_tag="RH057_001458"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HDV3127853.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(23450..24883)
                     /gene="glgA"
                     /locus_tag="RH057_001459"
     CDS             complement(23450..24883)
                     /gene="glgA"
                     /locus_tag="RH057_001459"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HDV3127854.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHAHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KRQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(24883..26178)
                     /gene="glgC"
                     /locus_tag="RH057_001460"
     CDS             complement(24883..26178)
                     /gene="glgC"
                     /locus_tag="RH057_001460"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HDV3127855.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(26196..28169)
                     /gene="glgX"
                     /locus_tag="RH057_001461"
     CDS             complement(26196..28169)
                     /gene="glgX"
                     /locus_tag="RH057_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HDV3127856.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGHSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPGGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGSLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNTYCQDNQLTWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITAVWQGPAHGLCVFQR"
     gene            complement(28166..30352)
                     /gene="glgB"
                     /locus_tag="RH057_001462"
     CDS             complement(28166..30352)
                     /gene="glgB"
                     /locus_tag="RH057_001462"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HDV3127857.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPVYRQYHHDKLT
                     FGILYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SMHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(30625..31728)
                     /gene="asd"
                     /locus_tag="RH057_001463"
     CDS             complement(30625..31728)
                     /gene="asd"
                     /locus_tag="RH057_001463"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV3127858.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            31920..32513
                     /gene="yhgN"
                     /locus_tag="RH057_001464"
     CDS             31920..32513
                     /gene="yhgN"
                     /locus_tag="RH057_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HDV3127859.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(32570..33910)
                     /gene="gntU"
                     /locus_tag="RH057_001465"
     CDS             complement(32570..33910)
                     /gene="gntU"
                     /locus_tag="RH057_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HDV3127860.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILHETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGSATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(33914..34441)
                     /gene="gntK"
                     /locus_tag="RH057_001466"
     CDS             complement(33914..34441)
                     /gene="gntK"
                     /locus_tag="RH057_001466"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HDV3127861.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDAAFAMQRTNKVSLIVCSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(34580..35575)
                     /gene="gntR"
                     /locus_tag="RH057_001467"
     CDS             complement(34580..35575)
                     /gene="gntR"
                     /locus_tag="RH057_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HDV3127862.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHCHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(35799..36494)
                     /gene="yhhW"
                     /locus_tag="RH057_001468"
     CDS             complement(35799..36494)
                     /gene="yhhW"
                     /locus_tag="RH057_001468"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HDV3127863.1"
                     /translation="MIYFRKANERGHANHGWLDSWHTFSFANYYDPNFMGFSALRVIN
                     DDVIEAGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPNSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(36617..37654)
                     /locus_tag="RH057_001469"
     CDS             complement(36617..37654)
                     /locus_tag="RH057_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV3127864.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKPFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAK"
     gene            37986..38474
                     /gene="yhhY"
                     /locus_tag="RH057_001470"
     CDS             37986..38474
                     /gene="yhhY"
                     /locus_tag="RH057_001470"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3127865.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKYGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
BASE COUNT         9386 a        10456 c         9580 g         9140 t
ORIGIN      
        1 gaacatgtgc cagacgttgg cgcagcattg ctttggcaaa tccctccctt cgtttctccg
       61 cgataatatg accaacctct cataatttaa atttaccccg ctctggtgat tctcaaacgc
      121 cagatgttac ccgtatcatt cacatgggta ccaaacatac tcctgacatc tgactacaat
      181 aattagtttt agtgggtatc agtcgtggtg ccgcaatatc tctgttcccg attgggataa
      241 ttagagtttg tcgtcagaaa attgacgtta cccataacaa atgaaaggcc aggtaaatca
      301 tgccattagt cattgttgct atcggtgtaa tcttgttgtt gctcctgatg atccgcttca
      361 aaatgaacgg cttcatcgct ctcgtcctcg tggcgcttgc tgttggatta atgcaaggaa
      421 tgccgctgga taaagttatt ggctccatca aagccggtgt cggcgggacg ctcggtagcc
      481 ttgccctgat catgggtttt ggcgcaatgc tgggcaaaat gctggcagac tgcggtggcg
      541 cacaacgtat cgccaccacg ctgattgcca aatttggtaa aaagcacatc cagtgggcgg
      601 tggtgttaac cggttttacc gttggttttg ccctgttcta tgaagtgggc ttcgtgctga
      661 tgctgcctct ggtgtttacc atcgcggctt ccgcgaatat tccactgctg tatgttggtg
      721 taccaatggc ggctgcactg tctgtgaccc acggcttcct gccaccgcat ccgggcccga
      781 ctgcgattgc caccattttc aatgccgata tgggtaaaac cctgctgtac ggtactattc
      841 tggcaatccc gaccgtgatt ctcgccggtc cggtttacgc tcgcgtgttg aaaggtatcg
      901 ataagccaat cccggaaggt ctctacagcg cgaaaacctt cagcgaagaa gagatgccga
      961 gctttggcgt cagcgtctgg acttctctgg tgccggttgt gctgatggcg atgcgtgcga
     1021 ttgccgaaat gatcctgccg aaaggtcacg ctttcctgcc ggtagcggag ttcctcggtg
     1081 acccggtaat ggcaacgctg attgccgtgc tgattgcgat gttcaccttt ggtctgaacc
     1141 gtggtcgttc aatggatcag attaacgaca cgctggtttc ttccatcaaa atcattgcga
     1201 tgatgctgtt gatcatcggt ggtggcggtg cgttcaagca ggtgctggta gacagcggcg
     1261 tggacaaata cattgcttcc atgatgcacg aaaccaacat ttctccgctg ctgatggcct
     1321 ggtcgattgc tgccgtactg cgtatcgcgc tgggttccgc aaccgttgcg gcaatcactg
     1381 cgggtggtat cgcggcaccg ctgattgcaa cgacgggtgt tagcccagag ctgatggtta
     1441 ttgcggtggg ttccggtagt gtgattttct ctcatgtgaa cgatccgggc ttctggctgt
     1501 tcaaagagta ctttaacctg actatcggcg agaccatcaa atcctggtcg atgctggaaa
     1561 ccattatctc ggtgtgcggt ctggtaggct gtctgctgct gaatatggtg atttgataac
     1621 actgactgcc ggatgcatcg catccggcat caaccgcact ctacttcttc ttcgctgcag
     1681 ctctgcgccg tctgtccaaa tccttcagca acttgttcac gccatcatcg gcaaacatcg
     1741 tctcaagcgt tgcggaaagc ttgcgtcgcc agtttttata ctggtaactg gtgccaggaa
     1801 tattcaccgg ttcggccata tccagccagt cttccggctg tagtcctaac agagcactgt
     1861 tactgtcggc aatgtagcgc tgcaaaccac ggttcagcgt cggcgtcatc gacatcaacg
     1921 atgccttatg cccggcacgt tttggcagac aaccatattt atgcagtgca tccagcagcc
     1981 cttgcttcgc cagttcgcga tcctgataca gaccgcgcag taccacttca tccggataca
     2041 gccccagggt tttgcccagc gttagatccc cgctctccca gtaaccgcgt agcgtaggca
     2101 ggtcatgtgt cgccgcaacc gccatcgact gctccggata cgctttcggt gcacggaacg
     2161 tcttctcgtg gtcgttttcg aaatagagca ctttgtagga gtacacaccg ctgctgcgca
     2221 gcttaccgac aatctctacc ggtacggtac cgagatcttc accaatcacc atacagcgat
     2281 gacgtttact ttcgagtgcc agaatcgaga gcagatcatc caccggatag tgaacatacg
     2341 cgccctgatc tgccgtctcg ccatacggta tccaccacaa acgcagcatc gacatcacat
     2401 ggtcaattcg taatgcgccg cagttttgca tattggcacg caacagctcg ataaacggtt
     2461 cataggcacg cgcggtgatg atatgcgggt ccattggcgg taatccccag ttctgcccca
     2521 acgggccgag gatatccggc ggcgcgccaa ccgacgcttt caggcaatat agttcacggt
     2581 cacaccaggt ttccgcccca ccttccgcta cgccaaccgc cagatcacga tacaagccaa
     2641 tcggcatttc atagccctgg cttatctccc agcaggcggc aaactggctg taagccagcc
     2701 actgcaacca gagataaaaa tcgacgtcat tacgatgttc ttcgcagaac tgacgcactt
     2761 ctggtgaatc cacgttctga tacatctctg gccatgcagg ccagccccag cgcatttcgt
     2821 cctctttcac ttgctgggca tgtagcgcat caaaggctgc ctgccagaac aggctgtcgc
     2881 cctgctctgc aacaaactgg cgaaacgcgg tcatctgctc atcatcacgt tgcgcgaaac
     2941 ctttccacgc cattcgtaat gctgtcattt ttagggcggt aaccgtggag taatcgaccc
     3001 agtcggcatc gcgcgcctgt tgcagcgtct gttgcgtggt cggcaactgc caccaggcct
     3061 gagcctcttc gctaagatgg aaatcttcaa cggcgttaac gtcgatataa atcacattca
     3121 gccaacggcg agaagacggg ctgtatgggc tggcactctc cggatttgcc ggatagagcg
     3181 catgaatcgg gttcaggccg atgaacgacc cgccacgttt tgccacatcc accagcatcg
     3241 ctttgagatc gccaaaatcc ccaatacccc agtttttttc cgatcgcagc gtataaagct
     3301 gaacgcaggc accccacagc ttttgtttat tcagcaacgc ctgcggttcg taacagcgtt
     3361 tcggggcgac aatcacccgg caatgcgcgc gctggtcgtc ctgggtgagt gtcagcgtgt
     3421 gataaccttc cggcagcttc gtcggtagat tgaacgcttt gccccccgtt acatggcctt
     3481 tgtactgtgt tccttcttcg gtggtcagca gccagctata ttcgccgctg ccctccacca
     3541 ccatcggcat ttttttgccg ctggtataaa ccatgacatt cgggactggc gttaccgcca
     3601 ctttcgtggc ggtacgttga tgcatcgcgt caagcaaacg ccgtttggtt tcggcgctaa
     3661 tcgactgcgg tttaccgtgg gcattgatgt aattggggct aatccccgcc gccagcgcgg
     3721 cattatccag acgtttgctt tccatcgagc ttccttagcg ttttgcctgc cagatacgag
     3781 cctgataatc gcgaatagag cgatccgagc taaacatacc gcagcgggcg gtattgagga
     3841 tcgccgcgcg agtccaggcc tcctggtcgc ggtacagcac atccacctgc ttttgtgcct
     3901 caacataggc tgcgaaatcc gccatcacca gatacggatc gccgccttgt ttgccgatac
     3961 tgtgcagcat ctggtcgaag gcatgcttat cgccgtcgct gtatttaccg ctttccagct
     4021 ctttcaatac tgcgtccagc accttatctt tcttccgcca tttcaccggg tcgtagcctt
     4081 tggccagaat agccttcact tgttccacgg tatgaccaaa aataaagata ttttcttcac
     4141 cgactttctc ggcgatttca acgttcgcac catccagcgt accgacagta agcgcaccat
     4201 tgagcgccag tttcatattg ccggtaccgg aagcttcttt acctgcagtc gaaatttgtt
     4261 cggagatatc cgccgccggg atcagttttt ccgccgccga aacgcaataa tccggcagga
     4321 acaccacctt caacttatcg ccaaccagtg gatcgttgtt gatcacgtca gccactttgt
     4381 tgatcgcaaa gataatattc ttcgccaggt agtagcccgg tgccgctttc gcgccgaaga
     4441 ggaagacgcg cggtacgcga tcagcctgcg ggttttcacg aatttctttg tacaacgcca
     4501 gaatatgcag cagattcagg tgctggcgtt tgtactcatg cagacgtttg atctgaatat
     4561 cgaaaatcgc ctgtggattg atctcaatac cggtacgaac tttcacaaac tccgccagac
     4621 ggactttatt cgcctgcttg atctcgcgat attgctgacg gaatttcgca tcatcagcga
     4681 atttttccag attgatcagc tgatcgagat cgttagccca ctctttttgc agtgatttat
     4741 ccaacagagc cgccagtgca gggttgcact gtttgatcca gcgacgtggg gtaataccgt
     4801 tggtgacgtt atggaatttg ttcggccata gctggtgata ttccgggaac agatctttca
     4861 ccaccagatc cgagtgcagc gccgcaacac cgttcaccgc gaaaccgcca accacacaca
     4921 ggttcgccat atgcacttgt ttatcgtgca ccaccgccag tttggcccac actttttcat
     4981 cgcccggcca ggttttctcc accagcgttt taaagcgagt attaatttcg ttaataatct
     5041 gcatatggcg cggcagtaag cctttcacca gtttcacatc ccagcgttcc agcgcttctg
     5101 gcatcagggt atggttggtg taagcgaaag ttttgctggt aatggcccag gcgtcatccc
     5161 agctcatctg gtgctcatcg atcagcacgc gcagcagttc tggaatcgcg atagttgggt
     5221 gggtatcgtt cagctgaata acttcgtaat ccgccagttc gtgcagttta cgccccgcca
     5281 gatgatggcg acgcaaaata tccgctaccg aacaggcaca ctggaagtac tgctgcatca
     5341 agcgcagctt tttaccggca gtatggttgt cgtttggata gagaactttg gtcagttttt
     5401 ccgcattgat gccctgctgt tcggcacgca ggaaatcacc gtcgttaaat ttagtcagat
     5461 caaacggatg cgcgtgcgtc gcctgccaca gacgcagcgg ctgcgccacg ccattacgat
     5521 agccgacaac ggggagatcc cacgcttgac cggtaatggt aaactccggc tcccagcgtc
     5581 cgtctttcgt cactttaccg ccaatcccta cctgcacatc cagtgcttcg ttgtggcgga
     5641 accacgggta gttactgcga tgccagtcat ccggcgcttc aacctgtttg ccatcgacaa
     5701 aagactggcg gaacaaacca tattgatagt tcagaccgta acccgtcgca gactgaccga
     5761 cagttgccat tgagtcgagg aagcacgccg ccagacgtcc cagaccaccg ttacccagcg
     5821 ccgggtcgat ctcttcttcc agcaggtccg tcagattgat gtcataagcc ttcaacgaat
     5881 cctgtacatc ctgataccag ccgagattca acaggttgtt gcccgtcagg cgaccaatca
     5941 aaaactccat tgagatgtag ttaacatgtc gctgattcgc caccggcttg gcgaatggct
     6001 gagcacgcag catttcggcc agtgcttcac tcactgctag ccaccactgg cgaggagtca
     6061 tttcagccgc agaatttaag ccataacgct gccactgacg tgaaagcgct tcctgaaatt
     6121 gcttatcgtt aaaaataggt tgtgacatag gagttccact tttcttagat tttcaacaca
     6181 acgttatcgc tagtttgcca ggctcgatgt tgaccttcct catcctgcgg gggattaggc
     6241 agggaggagt tgcggggatg agcaaggaaa tgtgatctca accacttaaa gctagtgcaa
     6301 accacaggat tagcagcaaa tcaatgcaat acagcgcaga aaatctgtat ctaagtgcaa
     6361 aaaatggccg ttgcgtattt tcaaaaagcg gaaggtaact ctataaatta agtaaaggag
     6421 tgaaacagtt tcataagtaa aatatccagt gtgctccatc tcattcttaa tagatttatt
     6481 aagatcatct ttttagatgg cactttcatc aggaatgaag aagaaaccct tgcttaaatg
     6541 aatctgatga acataaggga aaccagtatt cacgctggat cagcgtcgtt ttaggtgagt
     6601 tgttaataaa gatttggaat tgtgacacag tgcaaattca gacacataaa aaaacgtcat
     6661 cgcttgcatt agaaaggttt ctggccgacc ttataaccat taattacgaa gcgcaaaaaa
     6721 aataatattt cctcattttc cacagtgaag tgattaacta tgctgattcc gtcaaaacta
     6781 agtcgtccgg ttcgactcga ccataccgtg gttcgtgagc gcctgctggc taaactttcc
     6841 ggcgcgaaca acttccggct ggcgctgatc acgagtcctg cgggctacgg aaagaccacc
     6901 ctcatttccc agtgggcggc aggcaaaaac gatatcggct ggtactcgct ggatgaaggt
     6961 gataaccagc aagagcgttt cgccagctat ctcattgccg ccgtgcagca ggcaaccaac
     7021 ggtcactgtg cgatatgtga gacgatggcg caaaaacggc aatatgccag cctgacgtca
     7081 ctcttcgccc agcttttcat tgagctggcg gaatggcata gcccacttta tctggtcatc
     7141 gatgactatc atctgatcac taatccagtg atccacgagt caatgcgctt ctttattcgc
     7201 catcaaccag aaaatctcac cctggtggtg ttgtcacgca accttccgca actgggcatt
     7261 gccaatctgc gtgttcgtga tcaactgctg gaaattggca gtcagcaact ggcatttacc
     7321 catcaggaag cgaagcagtt ttttgattgc cgtctgtcat cgccgattga agctgcagaa
     7381 agcagtcgga tttgcgatga cgtttccggt tgggcgacgg cactacagct aatcgccctc
     7441 tccgcccggc agaataccca ctcagcccat aagtcggcac gccgcctggc gggaatcaat
     7501 gccagccatc tttcggatta tctggtcgat gaggttttgg ataacgtcga tctcgcaacg
     7561 cgccattttc tgttgaaaag cgccattttg cgctcaatga acgatgccct catcacccgt
     7621 gtgaccggcg aagaaaacgg gcaaatgcgt ctcgaagaga ttgagcgtca ggggctgttt
     7681 ttacagcgga tggatgatac cggcgagtgg ttctgctatc acccgctgtt tggcaacttc
     7741 ctgcgccagc gctgccagtg ggaactggcg gcggagctgc cggaaatcca ccgtgccgcc
     7801 gcagaaagct ggatggccca gggatttccc agcgaagcga ttcatcatgc gctggcggca
     7861 ggcgatgcgc tgatgctgcg cgatattctg cttaatcacg cctggagttt gttcaaccat
     7921 agcgaactgt cgctgctgga agagtcgctt aaggccctgc cgtgggacag tttgctggaa
     7981 aatccgcagt tagtgttatt gcaggcgtgg ctaatgcaaa gccaacatcg ctatggcgaa
     8041 gttaacaccc tgctagcccg tgctgaacat gaaatcaagg acatcagaga aggcaccatg
     8101 cacgcagaat ttaacgctct gcgcgcccag gtggcgatta acgatggtaa tccggatgaa
     8161 gcggaacggc tggcaaaact ggcactggaa gagctgccgc cgggctggtt ctatagccgc
     8221 attgtggcaa cctcggtgct gggtgaagtg ctgcactgca aaggcgaatt gacccgctca
     8281 ctggcgctaa tgcagcaaac cgaacagatg gcacgccagc acgacgtctg gcactacgcc
     8341 ttatggagtt taatccagca aagtgaaatt ctgtttgccc aagggttcct gcaaaccgcg
     8401 tgggaaacac aggaaaaagc gttccagctg atcaacgagc agcatctgga acagctgccg
     8461 atgcatgagt ttctggtgcg catccgagca caactgttat gggcctgggc acggctggat
     8521 gaagccgaag cgtcggcgcg tagcgggatt gaagtcttgt cgtcttatca gccacagcaa
     8581 cagcttcaat gcctggcaat gttgattcaa tgctcgctgg cccgtggtga tttagataac
     8641 gcccgtagcc agctgaaccg tctggaaaac ctgctgggga atggcaaata tcacagcgac
     8701 tggatctcta acgccaacaa agtccgggtg atttactggc aaatgaccgg cgataaagcc
     8761 gccgctgcca actggttgcg tcatacggct aaaccggagt ttgcgaacaa ccacttcctg
     8821 caaggtcaat ggcgcaacat tgcccgcgca caaatcttgc tgggcgagtt tgaacctgcg
     8881 gaaattgttc tcgaagaact caatgaaaat gcccgcagtc tgcggttgat gagcgatctc
     8941 aaccgtaacc tgttgctgct taatcaactg tactggcagg ccggacgtaa aagtgacgcc
     9001 cagcgcgtgt tgctggacgc attaaaactg gcgaatcgca ccggatttat cagccatttt
     9061 gtcatcgaag gcgaagcgat ggcgcaacaa ctgcgccagc tgattcaact caatacgctg
     9121 ccggaactgg aacagcatcg cgcacagcgt attctgcgag aaatcaatca acatcatcgg
     9181 cataaattcg cccatttcga tgagaatttc gttgaacgtc tgctaaatca tccggaagtg
     9241 ccagaactga tccgcaccag cccgttgacc caacgtgaat ggcaggtcct ggggctgatt
     9301 tactccggtt acagcaatga acaaattgcg ggcgaactgg aagtggcggc aaccaccatc
     9361 aagacgcata tccgcaatct gtaccaaaaa ctcggcgtcg cccaccgtca ggctgccgtg
     9421 cagcacgcgc agaaactgct gaagatgatg ggttatgggg tgtgagttta gccggataat
     9481 gcattggatc cggtttacat ctttacatca ttcaatactc acccgcgtta cgccatctgt
     9541 ttctatcaaa ccaaaccgca ccggcaagaa acgctccacc accgcgatat tggtcagcag
     9601 atggcatgaa ggatgggcga ccttaaattc tcccgcgccc gccagcgcca tcggcagtac
     9661 cagttggtcg gcgagatatt cccccaccgc cgctgggctt gccaggtagc gtttcacctc
     9721 tttcactaac tgcgcggcta ccacctcggc actgacgcgc ttttcaccga cgacgaaaaa
     9781 gcgttcagtg atattttcac tttcgacttc cagcgagacg gtattacccg gcccctggtc
     9841 gcgcggcagg ttatgaatat tctgttcatg cagggaaaaa ctccccgcca gtgtagcgat
     9901 ttcacgctca gcaacatggc gcggcacacc agctaataga acttctccac gcatctgcac
     9961 aatgttcccg cgctcgccaa gttgcaaggt gttaaacgat gccacgggcg agacttccgt
    10021 tgccaccacg ccgcctccgg caggataaaa accgtgacgt aacagcgtgg tttgctgatg
    10081 aattcctatt ttcgccagca gcggctccag cacccggcgg ataaaatccg caggcggggc
    10141 cgacgggtta tcggtgcctc cgctcacttc aacacgcgaa ggtccatcgg caaaccacag
    10201 cgcgggcagc accgtttgca gcaccagcgt acagcttccg gcactaccga tagcaaagcg
    10261 gtactcgccg ccgcgcacgg tgccaggccg gaagaccaga cgctgcgacc ccagctccgc
    10321 gccttccacc gttgccctac aaatttccgc agccgctttt accgcggtca gatgctggcg
    10381 caacagcccc ggtttcgctc gcccggcacg aatgctggtg atggtaaatg gctggccggt
    10441 tatcatcgac aggctcagcg ccgagcgcag gatctgcccg ccaccttcgc cctgtgcgcc
    10501 atccagcgca atcatccttt tcatcattta tccttttacg cacaccacct gacgcagggt
    10561 atagataact tccaccagat cgctttgtgc cgccatcacc gcatcaatat ctttatacgc
    10621 catcgggatt tcgtcgatca cttcggcatc tttacggcat tccacatgcg cggtggcacg
    10681 aatttgatct tccacgctga acagtttttt cgctttagta cggctcatta cccgcccggc
    10741 accgtggctg cacgaacaga acgactcttc atttcccagc ccacggacga taaagctttt
    10801 cgctcccatc gaaccgggaa taattccata ttgaccagca cgcgcagaca ccgcgccttt
    10861 acgcgtcacg tagatctctt cgccaaagtg ctgttctttt tgcacatagt tgtggtgaca
    10921 gttgatctct tccatcgcca gcgtttgctg ttgtcttacc gttttctgcg taacgctctg
    10981 caacgctgtt actacgtttt ccatcattgc atcgcggtta aggctggcaa aaagctgcgc
    11041 ccaggccacc gctttcaggt aatcatcaaa gtattccgta ccttccataa agtacgccag
    11101 atcacgcgac ggcaacgttt caagctggtc ctgcatctct ttttgcgcca gatcgataaa
    11161 gtaagtcccg atggcgttac caattccgcg tgaaccggag tgcagcataa tccacacctg
    11221 atccgactca tcaagacaga tttcaataaa atggttaccc gttcccagcg ttcccagatg
    11281 tttatagtta ttggtattca ggaaacgcgg atatttttgc gttaaccact gataaccggc
    11341 ttccagctca gcccatttag catcgacgtt aacaggtgga ttttcccagg cacctttatc
    11401 acgtttacaa cggccagtgg tacgcccgtg cggcacggcc gtttcaatcg cctgacgcag
    11461 ctctgccagg ttttcaggca ggtcttccgc cgttaacgcg gtacgcagcg cgttcattcc
    11521 acagccaata tccacgccca ccgccgccgg aataatcgcc cctttggtcg ggatcacgct
    11581 accaatggtg gaacctttac ccaggtgtac atcaggcatt accgcaatat gtttgaaaat
    11641 aaacggcatc ttcgccgtat taataagttg ctgacgcgca tcggcctcta ccggcacgcc
    11701 tttggtccac atttttaccg gggcattttc agtggtcagt aattcgtaat tcattttgtt
    11761 tttctctttt cgttgtttgc tgtcctgata attgcaaccg tcgtgccaga aaattaaata
    11821 agcagcttaa ttttttaatt cattgtttta aaaaagatta tatctttacg tccgtaaccg
    11881 gagatttccc gcaaagccaa tttaccgata atgaaatatc gtcttttata aggatatcta
    11941 agatgcgtaa aacagtggct tttggctttg tcggtaccgt actggattat gccgggcgcg
    12001 gcagtcagcg ctggtcaaaa tggcgtccga cactctgttt atgccagcaa gaatcgttgg
    12061 tcatcgatcg actggaattg ttgcacgacg cccgctcgcg ctcgctattt gaaacgctta
    12121 aacgcgatat cgccagcgtt tcgccagaaa cagaagtggt gagcgttgag attgaactgc
    12181 ataacccgtg ggatttcgaa gaggtctacg cctgcctgca tgatttcgcc cgtggttacg
    12241 agtttcagcc agaaaaagaa gactatttaa ttcacatcac caccggtacc cacgtcgcgc
    12301 agatttgctg gtttctgctg gcagaagcgc gttacctgcc cgcccggctg atacaatctt
    12361 cacctccgcg caaaaaagaa cagccgcgcg gcgcaggtga agtgacgatt atcgatctcg
    12421 atttaagccg ttataacgcc atcgccagcc gctttgccga ggaacgccag caaacgcttg
    12481 attttcttaa gtccggcatt gccacgcgta acccccactt caaccgcatg attgagcaga
    12541 tcgaaaaagt ggcgatcaaa tcccgcgcgc cgattctgct taacggtcca accggcgcgg
    12601 gcaagtcatt tctggcgcga cgcatcttcg agttaaaaca ggcgcggcat cagtttagcg
    12661 gcgcgtttgt ggaagtgaac tgcgccaccc tgcgcggcga taccgccatg tcgacgctgt
    12721 ttggtcatgt aaaaggcgcg tttaccgggg cgcgggaatc tcgtgaaggt ttattacgca
    12781 gcgccaacgg cggaatgttg tttcttgatg agattggcga actgggcgca gacgaacaag
    12841 caatgctgct aaaagccatt gaagagaaaa ccttttaccc gtttggcagc gatcgccagg
    12901 tgagcagtga ttttcagctt atcgccggaa cggtgcgcga tttgcgccag ctggttgccg
    12961 aaggcaaatt tcgcgaagat ctgtacgcgc ggatcaatct ctggaccttc accctgccgg
    13021 gtctacgcca gcgccaggaa gatattgaac cgaaccttga ttatgaagtg gagcgccacg
    13081 ccacactcac tggcgacagc gtgcgtttta acaccgaagc gcggcgcgcc tggttggctt
    13141 ttgcgacctc tccccaggca acatggcgcg gtaactttcg cgaactttct gccagcgtca
    13201 cgcggatggc cacctttgcc actagcggac gcatcactct ggacgtggtt gaagatgaga
    13261 taaaccgtct gcgctataac tggcaggaga gccgcccctc cgcgcttacg gcgttgctgg
    13321 gcgcagaggc agaaaacatc gacctttttg acaggctgca actggaacac gtgatagctc
    13381 tctgtcgcca ggcaaagtcg ctttccgctg ccggacgcct gctttttgat gtttcgcgcc
    13441 agggcaaagc cagcgtcaat gacgcggatc ggctacgcaa atacctggcg cgttttggtc
    13501 tgacgtggga agccgtgcag gatcagcaca gctccagttg aatatgatgg tccgtcagca
    13561 cctgcatcac gcttactggc ggcggggcgt cggtgtagac ggcatctacc atgctgatgc
    13621 tgcccatatt gaccatcgcg ttacggccaa atttcgagtg atcgacaacc agcataacgt
    13681 ggcgcgagtt ctcaataatg gcgcgtttgg tgcgaacttc gtggtaatcg aactccagca
    13741 gcgagccgtc gctatcgatg ccgcttatcc ccagaatgcc gaaatcaagg cggaactggg
    13801 agataaaatc gagcgtcgct tcgccaatga tcccgccatc gcggctgcgt aattcgccac
    13861 cggcgagaat aatgcggaaa tcttctttta ccatcaacgt gttagcaaca ttgagattgt
    13921 tggtgacaat acgcaaattg ctgtgattaa gcagcgcgtg cgccaccgct tccggcgtgg
    13981 tgccgatatc gataaacagt gtcgagccat tggggatttg ctccgccact ttgcgggcaa
    14041 tcctctcttt ttcttcggtc tgggtggctt tacgatcgtg ccacggcgtg ttaaccgaac
    14101 tggaaggcag cgccgcaccg ccatggtgac gcaggatcag gttttgctcc gccagctcat
    14161 tgaggtcgcg gcgaatagtc tgcgggctga cggagaaatg ctctaccagc tcttcagtac
    14221 tgacataacc ctgctgttta accagttcga taataccgtt gtgacgttgt gtttgtttca
    14281 tttataaatc cctggaatta ttttcgtttt cgcgcattga gcgaatcaac aaaagccatc
    14341 gctaaaccca cggctaaccc ggcgatgtgt gctccgttcg ccatcgacat cccaaacaaa
    14401 tcaaaccatc cggcgacaat ccagatcagc gcaaagataa ttaacccacg ttgcaggtaa
    14461 atgccacttt gcggatcgcg ttcgccacgt agccagacgt agcccatcag cgcatacacc
    14521 acgccagaaa gcccgccaaa ccacggcccg ctgaattttt gctgcacata gccgcttaac
    14581 agggcgctaa tgagagtaat aacaattagc ttaccgctac cgaggcgttt ttccaccgca
    14641 ccgccgagat accaccacca gagcaggtta aagaggatat gcatcagcga gaagtgcatt
    14701 aacgcgtggg tgaagtaacg ccagaactca aatttcagtg ttggatcgaa tggccaggcc
    14761 agccataaca tcacttcctg atcgccgaga atttgcatgg cgataaacac caccacgcag
    14821 gcgatcatca ccacccaggt taccggacct gcgcgttcac gcaaggcagc aaagaaagga
    14881 taacggcgat aatgcagacc actgccggta tggcccgcct gccagctggc cgccagataa
    14941 cgcggatctg ccgggttttc gagaaaacgc gccagctccg cccgcacgcg ctcggcctgg
    15001 gactcatccg ccagccagac atcgctttgg ttatgttgtt gaatcgtgag gataacaccc
    15061 tgcgtcgcca tataatcaac aaacgcctgc gccacgcggg ggttagcaaa agaggtaatc
    15121 atcaacatcg ttgctgtcgc ttattccaca caaaagggga cagtataaag cgttacgcgc
    15181 cgtacgccac ctctgcggga aactgacgct gccaggcttc aaaaccgccg tcaatgctat
    15241 agaccacatc gtagccctgt tgcagcagat actgcgccgc gcctttgctg ctattgccgt
    15301 gataacacat caccatcacc ggagtgtcaa agtcgttatc acgcataaac gcgcccagcg
    15361 tgtcgttggt taaatggaaa gcctgcaccg catgccccat agcgaaactc tgtggatcgc
    15421 gaatatcgac cagcaccgcc tctttttcct gcaacttctg gtgcgcgtcg gcaacgttaa
    15481 tacattcgaa ctgatccatg cgtctctctt tctttacaaa caagtgggca aatttaccgc
    15541 acagtttacg tcgaagcggc agataaacgc cataatgtta tacatatcac tctaaaatgt
    15601 tttttcaatg ttacctaaag cgcgattctt tgctaatatg ttcgataacg aacatttatg
    15661 agctttaacg aaagtgaatg agggcagcat ggaaaccaaa gatctgattg tgataggtgg
    15721 cggcatcaat ggtgctggta tcgcggcaga cgccgctgga cgcggtttat ccgtgctgat
    15781 gctggaggcg caggatctcg cttgcgcgac ctcttccgcc agttcaaaac tcattcacgg
    15841 tggcctgcgc taccttgagc actatgaatt ccgcctggtc agcgaggcgc tggctgaacg
    15901 tgaagtgctg ctgaaaatgg ctccgcatat cgccttcccg atgcgttttc gcctgccaca
    15961 tcgtccgcat ctgcgcccgg cgtggatgat tcgcattggt ctgtttatgt acgatcatct
    16021 gggtaaacgc accagcctgc cgggatcaac tggtttgcgt tttggcgcaa attcagtgtt
    16081 aaaaccggaa attaagcgcg gattcgaata ttctgactgt tgggtagacg acgcccgtct
    16141 ggtactcgcc aacgcccaga tggtggtgcg taaaggcggc gaagtgctta ctcggactcg
    16201 cgccacctct gctcgccgcg aaaacggcct gtggattgtg gaagcggaag atatcgatac
    16261 cggcaaaaaa tatagctggc aagcgcgcgg cctggttaac gccaccggcc cgtgggtgaa
    16321 acagttcttc gacgagggga tgcatctgcc ttcgccttat ggcattcgcc tgatcaaagg
    16381 cagccatatt gtggtgccgc gtgtgcatac ccagaagcag gcctacattc tgcaaaacga
    16441 agataaacgt attgtgttcg tgatcccgtg gatggacgag ttttccatca tcggcaccac
    16501 cgatgtggaa tacaaaggtg atccgaaagc agtgaagatt gaagagagtg aaatcaatta
    16561 cctgctgaaa gtgtataaca cgcactttaa aaagcagtta agccgtgacg atatcgtctg
    16621 gacctactcc ggtgtgcgtc cgctgtgtga tgatgagtcc gactcgccgc aggctattac
    16681 ccgtgattac acccttgata tccatgatga aaacggcaaa gcaccgctgc tgtcggtatt
    16741 cggcggtaag ttgaccacct accgcaaact ggcggaacat gcgctggaaa aactgacgcc
    16801 gtattatcag ggcattggcc cggcgtggac gaaagagagc gtgctgccgg gtggtgccat
    16861 tgaaggcgac cgcgacgatt atgccgctcg cctgcgccgc cgctatccgt tcctgactga
    16921 atcgctggcg cgtcattacg ctcgcactta cggcagcaac agcgagctgc tgctcggcaa
    16981 tgcgggaacg gtaagcgatc tcggggaaga tttcggtcat gagttctacg aagcggagct
    17041 gaaatacctg gtggatcacg aatgggtccg ccgcgccgac gacgccctgt ggcgtcgcac
    17101 aaaacaaggc atgtggctaa atgcggatca acaatctcgt gtgagtcagt ggctggtgga
    17161 gtatacgcag cagaagttgt cgctggcgtc gtaaggatga tgcccggttg tgaagtgcaa
    17221 ccgggctatt tggattcctt agactattca ttgtaaataa ttgaataacg gcccctgagc
    17281 taaaatcgcc aaacttaagc acgttaccca atcgccccac ttcttcctct ggtttctatt
    17341 ttttctgcac caatggcaat aatatcccaa tgcaaaacca agctgtgcac taatcgggcc
    17401 gacaaaatca gtccaaggct gcggccaaaa aggggaaaaa ttcaggggaa gccagacaaa
    17461 cgtcagaaag aaatttcgga tattttccag caacgctgat ttctctggag aaatataaag
    17521 aatgaaattc gagagaatta accagaaata gaccggcgaa aagaccagcc agcaatgcag
    17581 tctgtttttt aattcaggag gcgttctgcg ggagaattgc caaccgatgc cagccattac
    17641 actgaaaaaa agtgcatatt caaccattaa ctcaagttgt agttcctgaa gagaaatacg
    17701 cacaaccagg agcggaaagg agaatattat ttctgccaga aaagttaacg tgaatataag
    17761 tacggctaag acaataaccg tcggccatac caataatggc acgataacaa aaccgagaat
    17821 tacaggggga atataaagcc ggaatttaat catttttatt ttcctggtga ttcggatgat
    17881 gcgtcataca tcgcgcataa acactcatca attatccagt cgtctaccca gacctgatgc
    17941 tgtgtgccct gttctggctg ctttggtggg ttctcaatcg cctgccgcgc gcgttctttc
    18001 aagatggatt gattgttgaa ggcccaggca ttgatttggg tggtaatgac gttatcgata
    18061 tcgtcctggt tatctctgat ataatcctca caatatctgt tcaataaaca ggcaagagaa
    18121 acggtcgacc aaaagccggg aactgaatta atggcatcca gcaatggcgc aagagcgata
    18181 tcggcacgga aaaacatttc cagcatatac tgtaattcga cagcttcatc gccggatacc
    18241 caatcagtca tatactggca ggcaaatcgt tgcaaaattt tccattcatc ctcacgccaa
    18301 ttttctttac tggcaaggtt tatacgccgt agagaaaata cttcgtgaat attgtcaaac
    18361 tgcccctgtg caacaaattc cagtaaacgc ggcagaaaat agcggacctc atcgctattt
    18421 tgcggatcag gccctgggct actattccat gcattgatta aggaatacga gatcgctcgt
    18481 aacgatgtac tccgtaatac cttttgttcc tgctgactca ggcaatattc acaacaaacc
    18541 acaaatttct gcggcaccgg ataacgacga aaaatatgat aaatctgctc aattaatgcc
    18601 tgcatggcgg ggctgggttg cataattacc ccctcgcccg ctgatcgcgt gtaaacggtt
    18661 gataaacaaa acgagcattc gccacgttca ggcgaataat ggcactacga gagagggaaa
    18721 agctggggcg aatgcaacta ttgcagtctc gacagctttc ttcagcgcaa ttttccactg
    18781 tgtaataacg agcgtagagt ttcagttccg cttgcttatc acgaaatccg cgtaaactgt
    18841 tatcgttatg aaatttccag gcatctttaa agacctgaaa aatgtagata tcctcaatcc
    18901 aggactcaca ttttttaagc gggcgctttg ctatgatcac tcgctcatcc gggtttagca
    18961 tcgccgtaaa ttgaacggtg aggtcatttg tcgtgttcag atcacattga acatctggta
    19021 catcactgat ataggggtca aatccaaagt cagcaccaca ggcacaaaga cggctaatgg
    19081 tggcggtatc gagggaaaat cccggccata cattgctgga cgttaagcca atatttaatg
    19141 tgacattata tcgaagcaat ccctcaagac cgtcagcacc gacgaacttt tccccttcat
    19201 ccagggcctg gttaatgagt tgctgaacaa tgccttcatc ccagtatgcc gatttgcagt
    19261 tggcaatcgt aatatccacg cctgtgaatg gatcagtacc gacaaggctt gcaaataatg
    19321 tattaactaa ttccattttt attatccgcc ttaatgcaac gttttataca tcggcacata
    19381 acctacatct tccaccaggc tctgcccctg cggcgttaaa aaccactcca ccaatttttg
    19441 tgtttctgac gtcatatttt cccgcgttac cataaaggca tcgacgatat agggatattt
    19501 gccgttgcga atgttttcgg cagtcggtgc aataccgtta atcgctagca gttttatatt
    19561 tttatcggcg ttcatttgtg ttgcgtaata acggaaggtg taacctattg cgttgtttgt
    19621 attacggtat tcggcaacga ctttaatcat tccctccatc atacttgcca cttctgtttc
    19681 ctgcggcgag atcatgcgga catttttcat gacctgggat tgcatcaccg tctggctacc
    19741 agagtcttcc gggcgttgcc aggtctggat ctcctgatcg ttaccgccaa cagtacgcca
    19801 gttggtaatt gcaccactga agatgtcacg cacctgttgt tcagtcaggg aattaaccgg
    19861 gttatctaca ttgacgatga aaacaaacgc ttcacgggca aaaggcgtgt aaatcaaagt
    19921 gacgcccgat tcctccgcgc gttttttctg cccaccggaa ggttgagcca caaagataat
    19981 atcggcatta cctttaacaa tcttgttata tgcttcggga gtacgagagt tcgccagata
    20041 ttcccattcg tgaaaatctt ccggcaaaac gcttagtgca taaaaggcag aggcataaat
    20101 ggggtacgcc gccgttgcgc catcaagtcg cggccagttt tgcgtgaact gaatttgcgg
    20161 tttaccacgc agtggtgtca gttgattatt gagtttatcg ggtcgccagg cccaggtatc
    20221 aatattttca cgcacaccga cgccgggatt aagctgttta tataaataag cctggtaacc
    20281 ggcgacaaaa ccaagcatga gaaggataaa caagagagaa ttacggcaac gtaacccgga
    20341 cgtgcccgtt aaaggatatc tggtcggcca gccgtatccc agcgaaaaac tgatatgcgc
    20401 gccaacaggt ataatgacta accaccagta ctcaccaccg actaatgagg ctaacaaaac
    20461 gcccagccag gggagcataa gatcagccaa ccaacccgct cccatctggc gaaattgata
    20521 atctgccaga tgcagcgcga ttatccagac ccccagggtt aataatgcgg gtactaaaaa
    20581 aagacattgt cgccagacgg ctatttctct tttttctggt gcactatcgt ccgcccacac
    20641 tcgccccgag aaaaatagca ttaatgcgcc cagcgccagc ataaaaatgt tgagcagaag
    20701 gtaaattgtt ggcgtgataa agatattaca aacttcaata ataccggcaa gtccgacacc
    20761 cagcatggca ataaccgccg ccaaaaactg cgccagaatg gggatgccga aaaaagtcat
    20821 taccagcgag gtcaaaatcc attttctgtt ttgcattatt ctttccattc tttctgaatg
    20881 gcgaaattat actccccgag tccccttgcc ccttctggac acttttccga aatgatggcg
    20941 aaaaaaacgg gaccctttgg ccccgttcta tttattagtg aaattacaat ctcaccggat
    21001 caatatgcca gatatgatcg gcgtactctt tgatagtacg gtcagaagag aagtagccca
    21061 tattggcaat gttcagcatc gcttttgcgg tccactcttc ctgaagctcg tagagttcgt
    21121 cgactttatc ctgacaatcg acatagctgc gataatccgc cagtacctgg tagtgatcgc
    21181 cgaagttgat cagcgaatca accagatcgc gatagcgacc cggatcttcc ggactgaata
    21241 caccgctgcc gatttgcgtc agcacctgat gcagctcctc atctttctcg tagtattcac
    21301 gcggtttgta gccctgacga cgcagttctt ccacttcttc cgctgtgtta ccaaagataa
    21361 agatattgtc agcaccgaca tgatccagca tctcgacatt cgcaccgtcc aacgtaccga
    21421 tagtcagcgc accgttaagc gcaaacttca tgttactggt gccggaagct tccgtccccg
    21481 ccagcgaaat ctgttcagac agatcggccg ccggaatgat caactgcgcc aggctaacgc
    21541 tgtagttcgg gatgaacacg actttcagct tgtcgccaat ctgcggatcg ttgttgatca
    21601 ctttcgctac gtcattgatc aaatgaataa tgtgcttcgc catgtaatag gccgaagccg
    21661 ccttaccgcc aaaaatattc acgcgcggta cccacttcgc atccgggtcg gccttgatgc
    21721 ggttatagcg ggtaatcaca tgcaacacat tcatcaattg acgtttgtat tcgtgaatac
    21781 gtttgatttg tacatcgaac aacgcctttg gattcaccac cacattcagc tgctgggcga
    21841 tatactctgc cagacgcttt ttgttctcca gcttcgcctg atgcacagcg tgattaacca
    21901 ttgggaaatc acagtgttgt tgcagctcat taagcaggct aaggtcggtg cgccagttac
    21961 ggcccaggtg ttcgtccagc acggctgaaa gcgatgggtt cgctaccgct agccagcgac
    22021 gcggcgtcac accgttggtg acgttggtga aacgacccgg gaagattttc gcaaagtcgg
    22081 caaacaacga ttgcaccatc agattagagt gcagttccga tacaccgtta actttgtggc
    22141 tcacaacaac cgccagccag gccatacgca cacgacgacc gttggattca tcaatgatcg
    22201 acgcccgtcc cagcagatcg gtatcgttcg gatactgttc ctgcaaggtt ttcaggaaat
    22261 agtcgttgat ttcaaagatg atctgcaggt gacgcggcag aattttaccc agcatatcaa
    22321 ccggccaggt ttccagcgcc tcgctcatca gcgtgtggtt agtgtaggag aagacctgac
    22381 aacacacctc aaacgcgtcg tcccagctaa attggtgctc atcgatcagc agacgcatca
    22441 tctcaggaat cgacagtacc ggatgggtat cattgagatg aatcgcgatt ttatccgcca
    22501 ggttatcgta ggttttatgc aactgataat ggcggcttaa aatgtcctga atggtcgagg
    22561 aaaccaggaa gtattcctga cgcaggcgca gctcacgccc ggagtaggtg gagtcatccg
    22621 gatacagtac gcgagatacg ttctcggagt ggtttttatc ttccactgcc gcgaagtagt
    22681 caccctggtt gaatttaccg aggttaattt cgctactggc ttgggcactc cacaaacgca
    22741 acgtgttggt cgcgtcggtg tcgtaaccag ggattatctg atcgtaagcg actcccagaa
    22801 tctcttcggt ttcaatccag cgcgtttttt taccttcctg ctgaatgcga ccaccaaaac
    22861 ggactttata gcgcgtgttg tggcgtttga attcccacgg gttaccgtat tccagccagt
    22921 agtctggcga ctctttctgg ctaccgttaa cgatgttctg cttgaacata ccgtagtcat
    22981 agcggatgcc gtaaccgcgc cccggcaacc ctaacgtcgc cagagaatca aggaagcaag
    23041 ccgccagacg tcccaggcca ccgttaccga ggcctgggtc attttcttca tcaatcagct
    23101 cttcgagatt taaccccatc gcttccagtg cgccctgtac atcttcgtaa attcctagcg
    23161 acaacatggc gttggagagc gtacggccaa tcaaaaactc catcgacagg tagtaaacct
    23221 gacgagtttc ttgcgacaac tgggcacggt ttgaacgtaa ccagcgctcc acgagacgat
    23281 cgcgcacagc aaataacgtt gcgttcagcc attcatgttt attggcgacg accggatcct
    23341 ttccaatcgt aaacatcagc ttgtaagcga tagagtgctt aagagcttct acgctaagcg
    23401 tgggcgatga atatgtaaac ggagcattca tataggcgtt tcctgaaaac tatttcaagc
    23461 gatagtaaag ctcacggtac gacttcgccg cgacctgcca gctaaaatcc attgccatag
    23521 cctgacgttg cacaaaccgc cacagtgaag gacgggacca cagtacaaaa gcacgtcgaa
    23581 tagcccgtaa cagcgaccag gcattactat cttcaaagac aaacccactg gcgacgccat
    23641 ctgcaaggtt ctcaagagaa cagtcagaaa ccgtatcagc aagcccaccg gtgcgccgca
    23701 ctaacggcag cgtaccgtac ttcaatccat aaagttgcgt taagccgcac ggttcaaaac
    23761 ggctgggcac cagaatgacg tccgcgccgc ccataatgcg atgcgaaaat gcttcgtgat
    23821 agccaatctg aacgcccacc tggccggggt attccgctgc cgccgcaagg aaaccttcct
    23881 gcagcaccgg atcgcccgcg ccgagtagcg ccagctgccc gccctgctcc agaagacccg
    23941 gtaaggcttc cagcaccagg tcgagacctt tctggctggt cagacggctc accactgcaa
    24001 aaagcggcac tttatcgtca accttaagcc ccattgcgat ttgtaactgg cgcttatttt
    24061 ccgctttatc ttccaacgta tcgcgggtgt aacgcgaggc caacagtaag tccgtctctg
    24121 gactccagat tttctcgtcc acgccgttca gtacgccgga aagacgccct tcacggtgac
    24181 gctgttgcaa cagaccttcc ataccgtagg caaactgcgg ttcggtgatc tcgcgagcgt
    24241 aggttggact gaccgccgta atgtgatcgg catagtacag accggccttc aggaaagaga
    24301 tttgtccgtt gaattccagc ccatgaatat taaagaatga ccatggcaat tggatgtcat
    24361 tcatatgatg tgcataaaac atgccttgat aggccaggtt gtgcacagta aacaccgact
    24421 tcgccggacg cccgcgcgcc gccagatacg caggcgcaag gcctgcatgc cagtcgtgcg
    24481 catgcaccac atcaggacgc cagaatgggt caagcccgct ggccatttct gccccaaccc
    24541 accccagcag cgcaaaacgc aatacgttgt cggtataggc aaataagttg gtatcgtgat
    24601 acgggcttcc cggacgatca tagagatgcg gcgcgtcaat caggtatatg ccaaccccgt
    24661 tgtaatgacc gaacaacagc gtgatatgtc cggcgaaggt atcacgacgg gatactacct
    24721 gcgcatcggt aacgccacgg cgaatatcgg gaaatgcagg caacagtacg cgagcgtcaa
    24781 cgccgtctgc gatttgtgct gcgggtaatg ccccaataac atcagccaga ccgccggttt
    24841 taagcagcgg gaacatctct gaacatacat gtaaaacctg cattatcgct cctgtttatg
    24901 ccctaacttc cgtagcattt cgcgcgttac cagcacgatg ccttcttctg aacgatagaa
    24961 acgacgtgca tcttcctctg cgttttcacc aatcaccatg ccttccggaa taacacaagc
    25021 acgatcgatg acgcagcggc gcagacggca cgagcgacct acccatactt ccggtaacaa
    25081 tacggcggaa tcaatgttgc agaatgaatt cacgcgaacg cgcgagaaca gaacggactg
    25141 caccaccacc gaaccggaga tcacacaacc gccggatacc agtgagttaa gggtcatccc
    25201 gtggctaccg gagcgatcct gcacgaattt cgctggcggt aatgattcat tgtaggtgcg
    25261 aattggccaa ttgcgatcgt acatatccag ttccggcacc acagaggcca gatcgaggtt
    25321 cgctttccag taagcttcca gcgtacccac atcgcgccag tacggctcgg catccgggtc
    25381 ggattgtacg caagagagcg ggaacgggtg cgcataggcc agaccggctt cggtgatctt
    25441 gggaatcaaa tctttgccaa agtcgtggct ggagttctca tcacgatcgt cttcttccag
    25501 cagttcatac agatagtcgg cgtcaaagac gtagataccc atactcgcca gagatttgct
    25561 cggatcgttc ggcattgacg gtgggttagc aggtttttca acgaattcga taattttatc
    25621 gttctcatca accgccataa cgccaaatgc ggaggcttct tcaatcggta ctggcataca
    25681 agcaacggtg caacgtgcgc ctttttcgac gtgatcgata agcatacgcg agtagtcttg
    25741 cttgtagata tggtcgcccg ccaggatcac cacgtattcc gctttataac ggcggataat
    25801 gtcgaggttt tgggtgaccg catctgcggt gccgcgatac cagttttccc ctttcattct
    25861 ctgctgtgct ggcagcagat cgacaaactc gttcatttct tcattgaaga atgaccagcc
    25921 gcgctgaatg tgctgcacca gagtgtggga ctggtactgg gtgatcacgc ccatacgacg
    25981 gatcccggag ttgatgcagt tagacagcgc aaagtcgata atgcggaact taccgccgaa
    26041 gtgtacggcc ggttttgctc gcttattggt taaatccttc aggcgggtac cacgtcctcc
    26101 cgccagtatc agggcaacag atttcaatgg cagctggcgc gccaacatta agtgatcgtt
    26161 cttctctaaa ctaaccatga ctaactcctt ttttatcatc tctggaacac acacaatccg
    26221 tgtgcaggtc cctgccagac agccgtaatc actgggttat cctctccagc gaatggggga
    26281 atggcgtgcc actccccagc aggtaaaaca atctctgtta cctcaagcgt ggcgttaatt
    26341 gcgatcaaaa agcgatccga gagcagaatt tgcagctgtt tcggcccgtt ttgccactca
    26401 tccgtgctta aaggttgagc atatcgattt agccaacgga cattgccgtc gccttcttcc
    26461 caccagcgat tctccaccaa agcgggaatg cgcttgcgca gatggattaa cgcggcggta
    26521 aatgcggtta aaccactgct tgcctgcgac cagtccaacc aggttaattg gttatcctga
    26581 cagtaggtat tgttattgcc atgctggctg tgaccatgtt cgtcaccggc cagtaacatc
    26641 ggcgtaccct gggagagcaa caacgttgtt aacagggcgt gaatgctgtc gcgccgccgt
    26701 tcaaccaggt caagagaacc gcctaaccct tctttaccat gattgttact gtaattgttg
    26761 ttggtcccgt cgcgattttc ttctccgttt gcttcattgt gtttatggtt gaagcaaacg
    26821 cagtcgcgaa gcgtaaaacc gtcatgcgcg gtgacgagat taatcgcggc actcggcaga
    26881 cgaccattac gtttaaaaac atcgctggag gcagcaaaac gcccggcaaa cgcccccaga
    26941 ggcaaatcat aatgtagcca gaaacgacgg gcagcatcgc ggaaatgatc gttccactcg
    27001 gcaaacagcg gcgggaaatt tcccacctga taaccaccag gagcgatatc ccacggttca
    27061 gcaattaact tcacctgcga gagcaccggg cagttctgga tagcggtaaa caacggcgca
    27121 tcctgacgga actctggcgt acggcccatg actgccgcca gatcaaagcg gaaaccatcg
    27181 acgtggcagg tttctaccca ataacgcagg caggcgctgg cataatccac caccgccgga
    27241 tgactcaaat tgagcgtgtt gccgcaaccg gtccagttgt gataatcgcc gtcttctctt
    27301 atccaataat agctacggtt atcgatccca cgcagcgaga ataacgggcc gtcgaggtcc
    27361 agttccgcac tatggttgag cacgatatca agaatgactt cgatacccgc tttatgcagt
    27421 gctttgattg catcgcgaaa ctcatccagc gccgtttctg gcgagcaggc atacgccgga
    27481 tgcagcgcaa acatcgccac cgggttgtaa ccccagtagt tacttagccc catgcgttgc
    27541 agacgtggtt cactggcaaa ctgcgccact ggcagcagtt ccagcgcggt aatgcccaat
    27601 tgtttcaaat agttgatcat caccggatgc ccgagggctt tataagtgcc acggatctcg
    27661 accgggatct ccgggtgcaa gtacgttaat cctttgacat gggcttcata aatgatggtg
    27721 ctgccccacg gcgtgcgcgg cggggcatca tcttcccagt catagtgatc aaccaccact
    27781 acgcatttcg gcgcaatggc ggcgttgtcg cgatagtcag gttcattatg accggcgtgc
    27841 agcagcgggt tatctttaaa ctccccgtca atttgccgcg cgcaaggatc aatcaacaac
    27901 ttcgccgggt taaagcgatg cccctcggcg ggttgccagg ggccatgaac gcgataacca
    27961 taacgcaaac ccgggcgcgc atccggcaga taaccgtgcc aaatgtcgcc actgtgccct
    28021 ggcaagtcat agcgatgttc ctggccattg gcgtcaaaga cacacagttc tacccgctcg
    28081 gcatgagcgg agaaaagtgt gaagttgacg ccctgaccgt cgtaatgcgc gccgagggga
    28141 gcgggtttgc caatggcgag ttgtgtcatt ctgcctcccg aaccagccag atagtggcca
    28201 gcggtggtag cgtcaggctt agtgaatgct gacgaccgtg gctggcaatc tcatcgctgt
    28261 gtaccgtgcc gccattgcct gcattactgc cgtgatagtg catggaatcg gtattgagga
    28321 tttcacgcca tttgcccggc tggtttatgc cgaagcgata atcatgacgc ggtaccggcg
    28381 taaagttact ggcaacgatg atttcgttac cctctttatc gcgacgcaca aagatcagca
    28441 ccgagcgttc tttgtcatcc accaccagcc attcaaagcc gtacgggtca aaatccagtt
    28501 catgcattgc tttatggtgg cggtaggtga ggttcagatc gcgcaccaga cgctggacac
    28561 cgtggtgcca gttatcgccg ccttccaaca gatgccagtc gaggctggcg tcatggttcc
    28621 actcgcggcc ctgggcaaat tcgttaccca tgaacagtag tttcttgccc gggaatgccc
    28681 acatccagcc atagtaggcg cgcaggttcg cgaatttctg ccatgcgtcg cccggcatgc
    28741 ggtcgagaat cgatttttta ccgtggacca cttcatcatg cgacaacggc aggacgaagt
    28801 tttcagtgta gttgtagaga atcccgaagg tcagtttatc gtgatgatac tgacgataaa
    28861 ccgggtcgag cttcatgtag tccagggtgt catgcatcca gccgaggttc cacttgtacc
    28921 agaagcccag accgcccata tcctgcggac gagaaacgcc agggaaatcg gtagactcct
    28981 cagccattgt caccgcaccg gaaacctgct caccaagaat acggttggta ttacgcaaga
    29041 attcaatcgc ttcaagattc tcgcgcccgc caaattcgtt cgggatccac tccccctctt
    29101 tacggctgta gtcgcgataa atcattgacg ccaccgcatc gacgcgcagc gcatcaatac
    29161 caaaacgttc aatccagtaa agcgcgttac cgacgaggaa gttactgact tcacggcgac
    29221 catagttgta gatcagcgtg ttccagtcct gatgatagcc ttcacgcgga tcgctgtgtt
    29281 catacaagtt cgtgccatca aattcggcaa gcgcaaagtc atcagtcggg aagtggcctg
    29341 gcacccagtc gagaatcacg ttcagaccag ctgcgtgtgc ggcatcaatg aaataacgga
    29401 agtcgtcgcg agtaccaaaa cggcgggttg gtgcatacag gccggttggc tgataacccc
    29461 aactgccatc gaagggatgc tcgttaatgg gcagtagttc gaggtgggta aagcccatcc
    29521 atttagcata aggcaccagt tgatcggcca gctcgcggta gctcaaccag aaattgttgt
    29581 cggtgtggcg acgccaggaa cccaggtgaa cttcataaat agagattggc gcatcaaact
    29641 gattcgcttt tttgcgctct tcagtctgta caaccttttc cggcagcccg caaataagag
    29701 acgcggtttc cgggcgcatt tgcgcttcga aggcataagg gtcggacttc agacgcaagt
    29761 tgccattggc atcaatcatc tcgtatttat agagctgacc gttatgcgcc ccagggataa
    29821 acagttccca gatgccgctc tctttacgca ggcgcatcgg gtgacggcga ccgtcccagt
    29881 agttgaattg cccaaccacc gagacccgac gggcgtttgg agcccagaca gagaaacgcg
    29941 tacctgtgac gccatccata gtatctgcat gcgcgcctaa ggtttcatac gggcgcaggt
    30001 gagtaccttc agataatagc caggcatcca tttcctggat tagcggacca aaacggtaag
    30061 gatcatcaat caggttttgc tgaccatgcc agacaacagc caactgatag cggaaaaaat
    30121 tcttacgtcg cggaatgacg ccgctaaaga atccccgtga gtcgagacac tccagttttg
    30181 cgagtttgcg cccggttttc ggttcaatca cccacacatc ggtagcgtcg ggtaaaaggg
    30241 cacggacttc cagtcccgcg gtggttttat gcattcccag tacggaaaaa ggatccgcaa
    30301 aatggcctgc aattagcgcg ttaatcacgt ctctatcgat acgatcggac atgcttgtct
    30361 tcctgtttta ttgtgtcacc ccatccaatt ggatttttga cttctggttg tgacattttt
    30421 ttgacctgaa cggcgcagca ctctgtgcat cctctctgcg tcgtcctcac ttcaggtaag
    30481 gctgtgaata ctcatgtatt cagccaccct taaagaatag ccaatgctct atttaactcc
    30541 cggtaaatca tgaaacatct gcgcttactc ctgtattacg cactaacagg ggcggcatcg
    30601 cgccccagat ttaatgaata aagattacgc cagttgacga agcatccgac gcagcggctc
    30661 cgcggccccc cacagcagct ggtcgcccac ggtaaaggct gacaggaact ctggtcccat
    30721 attcagctta cgcaggcggc ctaccggcgt ggtcagcgtg ccggtaacgg cagctggggt
    30781 tagctcacgc atagtgattt cccgatcgtt cggaacgact ttcgcccacg gattgtgcgc
    30841 agccagcagt tcttccacgg tcggaataga cacatctttt ttcaatttaa tagtgaatgc
    30901 ctggctgtgg cagcgcaatg ccccgacacg cacacataaa ccatctaccg gaattacgga
    30961 agatgtgttg aggatcttgt tggtttccgc ctgccctttc cactcttcgc ggctctgacc
    31021 gttatcgagc tgtttgtcga tccacggaat caggctaccc gccagcggca cgccaaagtt
    31081 atccaccggc agctcaccgc tacgggttaa ggttgtgact ttgcgttcga tatcgagaat
    31141 agcagaggac ggggtcgcga gttcatctgc cacatggcca tacagatggc ccatctgggt
    31201 taataactca cgcatatgtc gcgcaccacc gccggaagcg gcctggtagg ttgcaacgga
    31261 cacccaatca acaagatcat tggcgaataa accacccaac gacatcaaca tcaggcttac
    31321 ggtacagtta ccgccaacaa aagtcctgat gccattattt aatccgtcgg taatgacgtc
    31381 ctgattgacg gggtcaagaa tgatgatggc gtcatctttc atgcgcagag acgatgctgc
    31441 gtcaatccag taaccttgcc atccgctttc acgaagcttt ggatagattt cgttggtata
    31501 atcgccgccc tgacaggtca caatgatatc gagggccttt agcgcctcca gatcaaaggc
    31561 atcctgaagt gtgccagtgg ttccgccaaa agacggcgca gcctggccaa gctgagaagt
    31621 agaaaagaag acagggcgaa tggcgtcgaa gtcgcgctct tcaaccatgc gttgcatgag
    31681 aacggagccg accataccgc gccagccgat aaaaccaaca tttttcataa gcgttttttc
    31741 ctgcaaagat gtgtgctgta taaatgtgcc ggtctcctct tggcacatct ttcaccatac
    31801 aaaaagcagc caaagtcgca agtgaaatta atcaatgata gcgaagccat cagtaatgcg
    31861 acttatcctg ctttgttagc acgcagaaag tccgcggcaa ttatcaggga atttgagtta
    31921 tgaatgaaat catttctgca gcagttttat tgatcctgat tatggatccg ctcggaaacc
    31981 tacctatttt catgtccgta ctgaaacata ctgaaccgaa aagacggcgg gcaatcatgg
    32041 tgcgagagtt gcttattgct ctcctggtga tgctggtgtt cctgtttgcg ggtgagaaaa
    32101 ttctggcatt tctcagcctg cgagcagaaa ccgtctccat ttctggcggc atcattctgt
    32161 ttctaatcgc cattaagatg attttcccca gcgcttcagg aaatagcagc gggcttccgg
    32221 caggtgaaga gccatttatc gtgccgttgg caattccgtt agtcgccggg ccgactattc
    32281 tcgccacgct gatgttgttg tctcatcagt acccgaatca gatggggcat ctggtgattg
    32341 ctctgctgct ggcctggggc ggcacctttg tcatcctgct acagtcttcg ctatttttac
    32401 gtctgctggg cgagaaaggg gtgaacgcac ttgaacgcct gatgggattg attctggtga
    32461 tgatggcaac ccagatgttc ctcgacggca ttcgaatgtg gatgaagggg taacgaggag
    32521 agtttttcga aaagaacccg gtagcgtcac aactaccggg cgaacaaact taactcaaca
    32581 gctggaacgc aatcatccca acgatggcac cgacagtgcc gaggatggtt tccatcatgg
    32641 tccaggtttt cagcgtttcg gcttcggtcg cgccggtaaa tttaccgaac aaccagaaac
    32701 cggcgtcgtt aacgtggctg acaacaatcg aaccaccagc gatacaaatc gacagcgccg
    32761 ccatttgcgc accggagtag ttcagttgtt caataaccgg catcaccagt cctaccgccg
    32821 ttaaacaggc tacggtggca gaaccctgaa tgatgcgcac tgcagcggcc agcacgaagc
    32881 aggtgatggc tatcggcagg cccatgccag ttaacgcttc gcccagtgcc ggacctacgc
    32941 cagagtcaac cagcacctgt ttgaacacgc cacctgcacc aatcaccagc agaatgatcc
    33001 ccgccggttg cagcgcgtga ccgcaaatct ccatcacttt gtcttttggc atgccctgac
    33061 gcattgccag gccataaatc gctaccagac aagcaaccag aatcgcggta aacggatgac
    33121 caataaactc gaaccattcg taagcggtag agccttctgg cacaaaacgc gcggcaatgg
    33181 ttttcagccc caccagcacc agcggcaaca ggatcagcga caggctgaat ccgaaagatg
    33241 gcatcttgcc ttcgccgaga tgcggttcgc tgatgtcgtc aggaatatgc aactccacgt
    33301 agcggctgat gaaattaccc cacagcggcc cggcaataat cattcccgga attgccgcac
    33361 acaggccaat caggatcatc cagccaaaat cggcgttcat ctgcgatgcc agcagcattg
    33421 gcgctggccc tggcaccagg aacgccgccg ctgccgccac gcctgcgaat aatgggatta
    33481 ccagcttcac caggttcgta ccggtgtggc gcgccattga gaaagcaacg ctaatcagca
    33541 gaacaatcgc cacttcaaag aacagcggta gcgcacagac cagccccgca aggccgatgg
    33601 cataatgcgc gcggctgtga ccgaaggatt taagcatttt gacggcaatc tgatcgactg
    33661 cgccggtttc atgtaagatc ttgccaaaca tagctcccag ggcgacaacc accgccagga
    33721 agccgagggt gcctcccatc cctttttcca tcgtcgctgc gattttatcg agcggcatac
    33781 cggaaaaaag gccagccccc atggacacca ccatcaaagc caggaaagcg tgcatacgcg
    33841 ccttcatgac taaaaacagc agcagtaaaa cagaccctac tgctgttaaa acaagcgtta
    33901 atgtagtcac tacttatttg ccttttttaa taacctcaat ggtgcttgcc acaacacctt
    33961 ccagcggttg atcgatatcc accaccagta catcagtttc gtccgcaccc ggctcctgca
    34021 gcgtttcaaa ctgcgtcacc aacatttggg ttttaaagaa atggcctttg cgcgctttca
    34081 ggcggctttc aatcacgtca aaatcgcctt tcaggtagat gaaagagaga ttcggattac
    34141 cttcacgcag caagtcgcga tagtgttttt tcaatgcaga acagacgatc agtgacactt
    34201 tattagtgcg ctgcatagca aacgcggcgt cgttcagcgc ctgcaaccac ggtttgcgat
    34261 cgtcgtcatt cagcggttcg ccagacgcca ttttttcgat attgcgccgt ggatggagga
    34321 aatcgccatc aagaaacgcg gcatgaagtt gatgcgccac ttcactggcg accgcagatt
    34381 tgccgctgcc cgatacgccc atcaagacgt aaatgtggtg atcatggtta gtcgtgctca
    34441 aagtggtgcc cccacaatac aagaattaaa aatgttacgg gtaactgtta tcggtaacat
    34501 tgtccagccg gacaatatca gaagcaatat ccatccgtgc cttaagtgta taagtgtgag
    34561 ctacttcaaa tttgtaggct taaatagatc cgcccggtga caaggtgaaa cctaaatcta
    34621 acattttcgg tgtcacagat tcgccacgaa tacgcgccag caggcgttca gcgccaatac
    34681 tgcccatccg ctcacgcggc gtcagcacgc tcgctaaccg cggctccatc acctggccaa
    34741 tgtcatgacc gtggaaaccg gcaatcgcca tatcgtcagg aaccttcaat cccagacgct
    34801 gacattcaaa cgccgcgccg accgccaggt catcgttggt acagaacacg ccatccagct
    34861 gcggatattc ccgccgcgcc tggcgaatca gttcaatacc ggaagagtaa gaagaagatt
    34921 gctcaaccat cacgctatac ggcaccagac ctgcatccag catcgcctgt tcgtatccct
    34981 tctgtttgat gatagtacgt tcgtcgagac gcgcgccgag ataggcaatg tggcaatgcc
    35041 cgcgagcaat aatggcggtg gtcatctggc gtgccgcttc aaagttatca aaaccgacgg
    35101 cgatatcgag gcacggcgac tggctgtcca tgagttccac caccgggatc cccgccactt
    35161 caatcatctt taaggtgcgc ggcgtgtggg tacgttcggt gaggatcagt ccgtcgatat
    35221 tccatgagag catggattcg agacgttctt gctccatttc cggtttataa ccgtagtgcg
    35281 ccagcatggt ctgataaccg tgcgcgtcgg tgacgctttc gattccgcgt aatacttccg
    35341 cgaaaacctg gttggtgaga gaaggtaaca ggacgccaat cgcccggctg gtggcgttag
    35401 agaggatatc gggcgcacga ttgggaatat agcccagttc atcaagagcg gcggcaatct
    35461 tgccgcgtag agcgacggaa acctgctccg ggttgcgtaa aaaacggctg accgtcattt
    35521 tggtcacgcc tacacggtca gccacatcct gaagtacggg tcttttcttt ttcatcgtcc
    35581 tgaaggtaca aaagagatag attctcttag tttaacacgg acgcatcaga accttcccgg
    35641 ggaaaacagt gcttgtttac aattattaga tatacactaa atcaattgca tcgcattgtg
    35701 ctaacgacgc ggcaaaaccc cggaagcgtg catcagcacg cgaccggagc gagcgaggaa
    35761 gcctgcaaaa aggcaggctt caaagatgca gttgagtttt aaaccggcgg cagatcgaac
    35821 agtaacactt cgctgtcgct atccgcatgg atggagattg cctgctcatc ccagattgcc
    35881 agaccatcgc tggtcgaggc tttcacgccg ttaatggtga cattgccttt caccacctgg
    35941 atccagacgc ggcgttcagc ggcaatctga tgcaccgact gctcatcttt cagcaacgcc
    36001 caacgataca gttccatatc ctgatgcact ttcagcgaac catctcgcgc atccggcgag
    36061 agcaccagct gtttgccctg cacggcatcg aagcgacgct gttcataacg cggcgtaatg
    36121 ccattttctt caggcatgat ccagatctga tacagatgca gacgctcggt gctgtttggg
    36181 ttgtactctg agtgacgaat acccgtaccc gcactcataa tctggaactc acccgccgga
    36241 acctgctctt tattgcccat gctgtcctga tgctcaacag taccttccag cacgtaggtc
    36301 aaaatttcca tatctttatg cgggtgagtg ccgaagccct gccctgcttc aatcacgtcg
    36361 tcgttaatca cgcgcagcgc ggagaagccc ataaagttcg gatcgtaata gttggcaaaa
    36421 gagaaagtat gccaggagtc cagccagcca tgatttgcat gaccacgttc atttgctttg
    36481 cggaagtaga tcatttgtat tcgccccctg aatgatttcg atgggcttag tgtggacccg
    36541 atctgcctgg gatgatagag ggtgaaaatt gacccctctg ttcaaaaaaa ttgaactatt
    36601 cgaggggcca tcaaacttac ttagcgagag ttactgtgga gggagaggct tgctcaaatc
    36661 cgcgttcaag gatttccaga ttggtaagaa cttcagattc cttgacgtaa tttggcgcac
    36721 cgtgggtgat ggtttgatac aacgcatcat aaacgcgccc gtaatcgccc atctccggct
    36781 tcatctcttc tctgaccgtc acgccctcgt cattgacata ctccagcaca ccgaccgaat
    36841 catccgctgc gaatcccggt tcgcccggca taatattagc cttcaggctg gtttcctgct
    36901 ggtcgatacc gtatttaata aacgaacctt tcttaccgtg aacgataaat ttcggataat
    36961 cgattttcac cagatggctg gttttgacga tggctttcag gtcgccataa aacagttgcg
    37021 cttcaaaggt gtcgtcagga ttggctttat tacgcaggct gcggatgtca taagcgacgt
    37081 gatccgggcg accgaacaga gaaataatct ggtccatcgt atgcacacca aggccataaa
    37141 acgcgccatc ctgcggcagc ccaggtttgg tctctgccac cgggcggtaa tagtcaaaat
    37201 ggctttccac ttcaacaatc tctcccaact tgccactttc aatcgctttt ttcgctgtca
    37261 ggaagcagga gtcaaagcga cgattctgat acggcgtgac ggtcagccct ttgcttttcg
    37321 ccaacgcaaa cagctccttc gcctgggcaa gcgtcggggt gaacggtttt tcgaccagca
    37381 catttttccc ggcttccagc gcgcgtttcg cgtactcgaa atggctgtcc gcgtgggtgc
    37441 agacaacaac cagcttaaca tcgggatcgt ttagtacttc gtcgagatcg ctggtgaaat
    37501 ggatatggga ataaatggga gcctgttctt ccggcttcgc atggcgacga aaaatatgcg
    37561 cgacatgcca gctatccttg cggttaagta catacggcag atggtaacgg gtggtgcttt
    37621 tgccgaagcc aataaaggcg cagttgatga ccatgatttc gtccttttta aggtggttat
    37681 ttacacctta gcgcaaagcg gacgtggttc ctactggagt gcgcataacg aacacaagca
    37741 ctcccgtgga taaattgaga acgaaagatc aaaaaaaagc cagcacccgg ctggctaagt
    37801 aatactggaa gcaatgtgag caatgtcgtg ctttcaggtt ctccgcgagg gtcttcctga
    37861 tcgcgagaca ataataatca ttctcattcg cacttgtcca acactttttg caaaaaaatg
    37921 catttgactc gcatttgaaa gtcaatgatg ttgaaaggga catttacccc aaagaggaca
    37981 aaggaatgag tgagatagta atacgccacg cagaaacacg ggattacgag gccatcaggc
    38041 agattcacgc ccagccggag gtgtattgca acacactaca ggtgcctcat ccttccgatc
    38101 atatgtggca ggagcgactc gccgatcgtc ccggcatcaa gcaactcgtc gcctgtattg
    38161 atggagacgt cgtgggccat ctcaccattg acgtgcaaca gcgcccacgc cgcagtcatg
    38221 ttgccgattt tggtatctgt gtcgactctc gttggaagaa ccgcggcgtc gccagcgccc
    38281 tgatgcgaga gatgattgaa atgtgcgaca actggttgcg ggtagatcgc attgaactaa
    38341 ccgtgtttgt cgataacgcc ccggcaatta aggtctataa aaaatacggc tttgaaattg
    38401 aagggactgg taagaagtac gcattgcgta atggtgaata tgtcgatgca tattatatgg
    38461 cgcgggtgaa gtaagatagt gccctttttc tgagatggaa aaagggtgtc attcaaaatc
    38521 gacatacctt cctttaaggt atttatttgc ccaatacata aa
//
LOCUS       DAPHDW010000029        38453 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.029, whole genome shotgun sequence.
ACCESSION   DAPHDW010000029 DAPHDW010000000
VERSION     DAPHDW010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 38453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.029"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            113..2356
                     /gene="pdeF"
                     /locus_tag="RH057_001471"
     CDS             113..2356
                     /gene="pdeF"
                     /locus_tag="RH057_001471"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HDV3127866.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGIALGIFVRKYAELGFYETLSLTANFIIIIIFCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIMLRIQYKQKLSHWLSPLLE
                     PGEDVYQLSGNDLALRLNTESHQERITALDSHLKQFRFFWDGMPMQPQIGVSYCYVRS
                     PVNHIYLLLGELNTVAELSIVTNAPENMQRRGAMYLQRELKDKVAMMNRLQQALEHNH
                     FFLMAQPITGMRGDVYHEILLRMKGENDELIGPDSFLPVAHEFGLSSSIDMWVIEHTL
                     QFMAENRAKMPAHRFAINLSPTSVCQARFPVEVSQLLAKYQIEAWQLIFEVTESNALT
                     NVKQAQITLQHLQELGCQIAIDDFGTGYASYARLKNVNADLLKIDGSFIRNIVSNSLD
                     YQIVASICHLARMKKMRVVAEYVENEEIREAVLSLGIDYMQGYLIGKPQPLIDTLNEI
                     EPIRESA"
     gene            complement(2395..3936)
                     /gene="ppx"
                     /locus_tag="RH057_001472"
     CDS             complement(2395..3936)
                     /gene="ppx"
                     /locus_tag="RH057_001472"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HDV3127867.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEVLRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(3941..6007)
                     /gene="ppk1"
                     /locus_tag="RH057_001473"
     CDS             complement(3941..6007)
                     /gene="ppk1"
                     /locus_tag="RH057_001473"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HDV3127868.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNSHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRGDTIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            complement(6178..6816)
                     /gene="purN"
                     /locus_tag="RH057_001474"
     CDS             complement(6178..6816)
                     /gene="purN"
                     /locus_tag="RH057_001474"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HDV3127869.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            complement(6816..7853)
                     /gene="purM"
                     /locus_tag="RH057_001475"
     CDS             complement(6816..7853)
                     /gene="purM"
                     /locus_tag="RH057_001475"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HDV3127870.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            8178..8804
                     /gene="upp"
                     /locus_tag="RH057_001476"
     CDS             8178..8804
                     /gene="upp"
                     /locus_tag="RH057_001476"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HDV3127871.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            8890..10179
                     /gene="uraA"
                     /locus_tag="RH057_001477"
     CDS             8890..10179
                     /gene="uraA"
                     /locus_tag="RH057_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HDV3127872.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPDSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            10229..10975
                     /gene="hda"
                     /locus_tag="RH057_001478"
     CDS             10229..10975
                     /gene="hda"
                     /locus_tag="RH057_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HDV3127873.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(11113..11472)
                     /gene="arsC"
                     /locus_tag="RH057_001479"
     CDS             complement(11113..11472)
                     /gene="arsC"
                     /locus_tag="RH057_001479"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311384.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HDV3127874.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIVVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(11493..12956)
                     /gene="bepA"
                     /locus_tag="RH057_001480"
     CDS             complement(11493..12956)
                     /gene="bepA"
                     /locus_tag="RH057_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HDV3127875.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQERFKPYTKM"
     gene            13169..14230
                     /locus_tag="RH057_001481"
     CDS             13169..14230
                     /locus_tag="RH057_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV3127876.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPALMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            complement(14268..14492)
                     /locus_tag="RH057_001482"
     CDS             complement(14268..14492)
                     /locus_tag="RH057_001482"
                     /inference="COORDINATES: protein motif:HMM:NF013397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HDV3127877.1"
                     /translation="MYRQFVCDSVRHCHSPFCPTPLRQLAHSSADNFPALTVSHFITA
                     NLLPVMLGNIIGGAVLVSICYRAIYLRQES"
     gene            complement(14384..15115)
                     /locus_tag="RH057_001483"
     CDS             complement(14384..15115)
                     /locus_tag="RH057_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001698835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
                     /protein_id="HDV3127878.1"
                     /translation="MRNKLSFDLQLSARKAAIAERIASHKIARSKVSVFLMAMSAGVF
                     MAIGFTFYLSVIADAPSSQALTHLVGGLCFTLGFILLAVCGTSLFTSSVMTVMAKSRG
                     VISWRTWLINALLVACGNLAGIACFSLLIWFSGLVMSENAMWGVAVLHCAEGKMHHTF
                     TESVSLGIMCNLMVCLALWMSYCGRSLCDKIVAMILPITLFVASGFEHCIANLFVIPF
                     AIAIRHFAPRPFGNWRTVAQTIFRH"
     gene            complement(15137..17149)
                     /gene="hyfR"
                     /locus_tag="RH057_001484"
     CDS             complement(15137..17149)
                     /gene="hyfR"
                     /locus_tag="RH057_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HDV3127879.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSLVQLIELHVLKSNFYYRYHDDGSDVTATVEYQGEMVDYSRHAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEEQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PRNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADAWRSMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRRDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRAIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNNLNLHLNVRQSRLLPTLNEDSALRSSMAQLLHPTTPENDEEER
                     QRIVQVLRETNGIVAGPRGAATRLGMKRTTLLSRMQRLGISVREVL"
     gene            complement(17179..17592)
                     /gene="hyfJ"
                     /locus_tag="RH057_001485"
     CDS             complement(17179..17592)
                     /gene="hyfJ"
                     /locus_tag="RH057_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HDV3127880.1"
                     /translation="MAEECGEIVIWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLAQVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFALLA
                     DDATSVWQARSIQFIHLLDEIVQEPAIYLMARKIA"
     gene            complement(17585..18343)
                     /gene="hyfI"
                     /locus_tag="RH057_001486"
     CDS             complement(17585..18343)
                     /gene="hyfI"
                     /locus_tag="RH057_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HDV3127881.1"
                     /translation="MSPVLTQHVSQPITLDEQTQKMKQHLLQDIRRSAYVYRVDCGGC
                     NACEIEIFAAITPVFDAERFGIKVISSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLREADDPRLNSIVQQLFRVLRGLHG"
     gene            complement(18340..18885)
                     /gene="hyfH"
                     /locus_tag="RH057_001487"
     CDS             complement(18340..18885)
                     /gene="hyfH"
                     /locus_tag="RH057_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HDV3127882.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSHTWQLYLRRCIYCGRCEEVCPTRAIQLTNNFELTVTN
                     KADLYTRATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLLNDDTDVPLVAKEQL"
     gene            complement(18895..20610)
                     /gene="hyfG"
                     /locus_tag="RH057_001488"
     CDS             complement(18895..20610)
                     /gene="hyfG"
                     /locus_tag="RH057_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
                     /protein_id="HDV3127883.1"
                     /translation="MNVNSSSNRGEAILAALKTQFPGAVLDEERQTPEQVTITVKINL
                     LPDVVHYLYYQHDGWLPVLFGNDERTLNGHYAVYYALSMEGAEKCWIVVKALVDADSR
                     EFPSVTPRVPAAVWGEREIRDMYGLIPVGLPDQRRLVLPDDWPEDMHPLRKDAMDYRL
                     RPEPTTDSETYAFINEGNSGARVIPVGPLHITSDEPGHFRLFVDGEQIVDADYRLFYV
                     HRGMEKLAETRMGYNEVTFLSDRVCGICGFAHSVAYTNSVENALGIEVPQRAHTIRSI
                     LLEVERLHSHLLNLGLSCHFVGFDTGFMQFFRVREKSMTMAELLTGSRKTYGLNLIGG
                     VRRDILKEQRLQTLKLVREMRADVSELVEMLLAMPNMEQRTQGIGILDRQIARDYSPV
                     GPLIRGSGFARDLRFDHPYADYGNIPKTLFTFTGGDVFSRVMVRVKETFDSLAMLEFA
                     LDNMPDTPLLTEGFSYKPHAFALGFVEAPRGEDVHWSMLGDNQKLFRWRCRAATYANW
                     PVLRYMLRGNTVSDAPLIIGSLDPCYSCTDRVTLVDVRKRQSKTVPYKEIERYGIDRN
                     RSPLK"
     gene            complement(20600..22185)
                     /gene="hyfF"
                     /locus_tag="RH057_001489"
                     /pseudo
     CDS             complement(20600..22185)
                     /gene="hyfF"
                     /locus_tag="RH057_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
     gene            complement(22190..22840)
                     /gene="hyfE"
                     /locus_tag="RH057_001490"
     CDS             complement(22190..22840)
                     /gene="hyfE"
                     /locus_tag="RH057_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HDV3127884.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFAAEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            complement(22852..23959)
                     /locus_tag="RH057_001491"
                     /pseudo
     CDS             complement(22852..23959)
                     /locus_tag="RH057_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442177.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit D"
     gene            complement(23971..24081)
                     /locus_tag="RH057_001492"
     CDS             complement(23971..24081)
                     /locus_tag="RH057_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoin racemase"
                     /protein_id="HDV3127885.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGFCSPH"
     gene            complement(24098..25045)
                     /gene="hyfC"
                     /locus_tag="RH057_001493"
     CDS             complement(24098..25045)
                     /gene="hyfC"
                     /locus_tag="RH057_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311372.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HDV3127886.1"
                     /translation="MRQTLYDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPISSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLATGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            complement(25056..27073)
                     /gene="hyfB"
                     /locus_tag="RH057_001494"
                     /pseudo
     CDS             complement(25056..27073)
                     /gene="hyfB"
                     /locus_tag="RH057_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            complement(27073..27690)
                     /gene="hyfA"
                     /locus_tag="RH057_001495"
     CDS             complement(27073..27690)
                     /gene="hyfA"
                     /locus_tag="RH057_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HDV3127887.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVLPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGEDPLSLTQEQH"
     gene            complement(27943..28413)
                     /gene="bcp"
                     /locus_tag="RH057_001496"
     CDS             complement(27943..28413)
                     /gene="bcp"
                     /locus_tag="RH057_001496"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HDV3127888.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            complement(28413..28985)
                     /locus_tag="RH057_001497"
     CDS             complement(28413..28985)
                     /locus_tag="RH057_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HDV3127889.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            29131..30009
                     /gene="dapA"
                     /locus_tag="RH057_001498"
     CDS             29131..30009
                     /gene="dapA"
                     /locus_tag="RH057_001498"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HDV3127890.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLELADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            30026..31060
                     /gene="bamC"
                     /locus_tag="RH057_001499"
     CDS             30026..31060
                     /gene="bamC"
                     /locus_tag="RH057_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HDV3127891.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VSVFQAAFSK"
     gene            31273..31986
                     /gene="purC"
                     /locus_tag="RH057_001500"
     CDS             31273..31986
                     /gene="purC"
                     /locus_tag="RH057_001500"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HDV3127892.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            32140..32367
                     /gene="vapB"
                     /locus_tag="RH057_001501"
     CDS             32140..32367
                     /gene="vapB"
                     /locus_tag="RH057_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HDV3127893.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAG
                     ESWDSWFDGENVSADFMDIRDQPAMQERESF"
     gene            32367..32765
                     /gene="vapC"
                     /locus_tag="RH057_001502"
     CDS             32367..32765
                     /gene="vapC"
                     /locus_tag="RH057_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_490590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HDV3127894.1"
                     /translation="MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIVVTNNTREFERVAGIRLEDWS"
     gene            32912..33775
                     /locus_tag="RH057_001503"
     CDS             32912..33775
                     /locus_tag="RH057_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HDV3127895.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVILATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            33790..35805
                     /gene="tmcA"
                     /locus_tag="RH057_001504"
     CDS             33790..35805
                     /gene="tmcA"
                     /locus_tag="RH057_001504"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HDV3127896.1"
                     /translation="MAELTAFHTLTAQMKREGIRRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQPDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDTDSLRWSDCPDPIAAPHFVQHFKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHTATGAPQPEQQQLLQQLLTMPSGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFDDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQYFLQAAGENEIAGALWLVDEGGLSQQLSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGQQLIAGALQYTRDLDYLSVSFGYTGELWRFWHRCG
                     FVLVRMGNHREASSGCYTAMALLPMSNAGKQLAEREHYRLRRDAQALAKWNGETLPVD
                     PLNDAVLSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNVSDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNDVRTERLRDRITQWQFFH"
     gene            35879..36577
                     /gene="ypfH"
                     /locus_tag="RH057_001505"
     CDS             35879..36577
                     /gene="ypfH"
                     /locus_tag="RH057_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV3127897.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDEAL
                     SGGKPGDDDVIEMM"
     gene            complement(36658..36858)
                     /locus_tag="RH057_001506"
     CDS             complement(36658..36858)
                     /locus_tag="RH057_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HDV3127898.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(36886..38013)
                     /gene="dapE"
                     /locus_tag="RH057_001507"
     CDS             complement(36886..38013)
                     /gene="dapE"
                     /locus_tag="RH057_001507"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HDV3127899.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMSRNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            complement(38017..38373)
                     /locus_tag="RH057_001508"
     CDS             complement(38017..38373)
                     /locus_tag="RH057_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HDV3127900.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLKG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
BASE COUNT         9352 a         9911 c        10119 g         9071 t
ORIGIN      
        1 gacacttaga atcaatgctc tctatagtga tgaataatca tcattcgaag tcaggtggga
       61 tgcctgtctg aatacacctc cttcaggatg tgggggattc tgctgagcat ctatgaaact
      121 gaatgcaact tatataaaaa tacgtgataa atggtggggg cttccgctgt tcctgccttc
      181 tttaatcttg cccattttcg cccacattaa tactttcgcg catatttctt ccggtgaggt
      241 ttttctcttt tatctgcctc tggcactgat gatcagcatg atgatgtttt tcagctgggc
      301 ggcattgcca gggatcgcct tagggatttt tgtccgcaaa tatgcagagc tgggttttta
      361 cgaaacgcta tcattaacgg ctaattttat tatcattatc attttctgtt ggggcggtta
      421 cagggtcttt actccccggc gtaacaacgt ttcacatggt gatacccgtt taatttccca
      481 gcgtatattc tggcagattg tgtttcctgc aacgctgttt ctgatacttt tccagtttgc
      541 tgcatttgta ggattactgg cgagcagaga aaatctggtc ggtgtcatgc cttttaacct
      601 cgggacttta atcaattatc aggccttact ggtgggtaat ctgatcggtg tcccgctgtg
      661 ctacttcatc attcgggtag tgcgaaatcc attttattta cgtagctatt attcgcaatt
      721 aaaacagcag gttgatgcca aagtcaccaa aaaagagttc gcgctctggc tactggcatt
      781 aggtgcttta ttgttgctgt tatgcatgcc gttaaatgaa aaaagcacaa tttttagcac
      841 caattatacc ttgtcattat tgctgcccct gatgatgtgg ggagcgatgc gctatggtta
      901 taagctgatt tcgctgctct gggcggtcgt gttgatgatc agcatccaca gctatcaaaa
      961 ttacattccc atttatcctg gctataccac gcagctgacc ataacctcct ccagttatct
     1021 ggtattctct tttattgtca attatatggc tgtactggca actcgtcagc gagcggtagt
     1081 cagacgcatt cagcggcttg cgtatgtgga cccggtggtt catctgccaa atgttcgcgc
     1141 cctgaatcgc gcgttgcgtg atgccccctg gtctgcgctt tgttatttac gcatccctgg
     1201 catggaaatg ctggttaaga actatggcat catgctgcgg attcaataca agcaaaaact
     1261 ttctcactgg ctgtcaccct tgctggaacc gggtgaagat gtttatcagc tttcgggtaa
     1321 cgatctcgcg ctgcggctga atacagaatc gcaccaggag cgcattaccg cactggatag
     1381 ccatctcaag caatttcgtt tcttttggga tggcatgccg atgcaaccgc agattggcgt
     1441 cagttactgc tatgtgcgct cgccagtgaa tcatatctac ctgctgctgg gagagctaaa
     1501 tacggtcgcc gaactttcca tcgtgaccaa cgccccggaa aatatgcagc gtcgcggggc
     1561 aatgtatttg caacgcgaat tgaaagataa agtcgcgatg atgaatcgac tacagcaggc
     1621 gctggaacac aaccattttt tcctgatggc ccagccgatt accggtatgc gtggtgatgt
     1681 ttaccatgaa attcttctgc gcatgaaagg tgagaatgat gaactgatcg gccccgatag
     1741 cttcttgccg gtcgcgcacg aatttggttt atcgtcgagt atcgacatgt gggtcattga
     1801 gcatacgctg caatttatgg ctgaaaacag agcgaagatg cccgctcacc gttttgctat
     1861 taatctgtct ccaacctcgg tatgtcaggc tcgttttcct gttgaagtca gtcagttgct
     1921 ggctaaatat cagattgaag cgtggcaact tatttttgaa gtcaccgaaa gtaatgctct
     1981 gaccaatgtt aagcaggcgc aaatcacctt gcagcatctt caggaattag gctgccagat
     2041 tgcgattgat gatttcggca ccggctacgc cagctatgca cggcttaaaa atgtgaatgc
     2101 cgatctgctt aaaattgacg gcagttttat ccgcaatatt gtgtcaaata gtctggatta
     2161 tcagatagtg gcatcgattt gccacctggc gcgaatgaag aaaatgcggg tagtggcaga
     2221 gtacgttgaa aacgaagaga tccgcgaggc ggtgctctct ttggggatcg attatatgca
     2281 gggttatctt attggtaagc cgcagccgtt aattgatacg ttgaatgaaa tcgaacccat
     2341 tcgcgaaagt gcctgaataa tgcgggccga catttctcgt cggcccgcaa agcattaagc
     2401 ggcgatttca ggtgtacttt cttcttcaat tttcaaccgc cagccagcca cgccttccca
     2461 gtattcttgc tccttttcca gatcaagcag taccagcgca ttctgactaa accagtcatg
     2521 cgggaaacgc agtgtccagt gactgtcatc agtaatcagt gtcaatgttg gcggtgtggt
     2581 ggttgcctga cgttgattgt ttagtaatac gccaaggcgc aatagctgta tcagtggcag
     2641 gaactgtttc ttcttaaaca aggtaaagcg cggcagatcg tcgagcttaa tcgctttacg
     2701 gtgatagcgc accagtgtcg ccatcatcag ctgctgttcc tgattaaaac ccggcaagtc
     2761 actgttttgc agaatataag cggagtggcg atgcaaaccg ctgtggttga tattcaaccc
     2821 gacctcatgc agcatggcgg cccatcgcag tagcgcctcc agttgcggat gcgccagctt
     2881 cggttgctgt tcccgccact gttcgtacat ttgcatagtg gtatccagca ctcgtcgggc
     2941 ctgttcgctg tcgatgtgat actggttggc gaggctgctg gcggtgcgac tacgcacatc
     3001 ctgatgacgg aaacgtcctt ccatttcata cagtacgcct tcgcgtaacg ccccgtcaga
     3061 aaggcgcagt tcacggatgg ctaaagcatc aaacacaccg cataaaatcg ccagtccagg
     3121 aacgaagact gttttccgct cttcggaaag acccggtaaa ctcagcgatg cgaaattacg
     3181 gtgacgtaaa acttctttta ccagtttttc cagacgttcc ggggtaatta tcccgtcttt
     3241 ctcgcccatt tccattaaca cttcatgggc ggcttttatg gtaccggaag cgcccattgc
     3301 cacgttccag ccctgaatac ggaattgcca ggttaaagtt tccagttttt gtgctgccgc
     3361 catacgagcg cgctgaaaat tctctttatt gatgaccccg ccagggaagt aaagctgggc
     3421 aaagctgaca caacccatcc ggcggctttc aacgagaatc ggttcgaaat tttcaccaat
     3481 caccagttcc gtagatccgc cgccaatatc aataaccagt ttgcgacctt tctccggttg
     3541 ggtatgttcc acgcccataa aaatcagacg ggcttcttca ttaccggaaa taatttcaat
     3601 cgggtaggga atgacctttt ccgcgcgttt cagaaagtca gtggcgttca gcgcctgacg
     3661 cagcgtatgg gtgccgacta tacagacgct ggcaggagaa aacccttgta gccgttcggc
     3721 aaacagcgac agacagttca aaccgcgcgt cattgcctct tcactcaaca tattatctgg
     3781 ccccaggccg tccgccagat gcacccgctg tttcaggcgg ccaataatct gcatggcacc
     3841 atctaccaca cgggctatga ccatgtgaaa actgtttgaa ccaagatcga ccgcagcaaa
     3901 ctcctgcgga cgaggggatt tatcgtgtat tggcataggg ttattcaggt tgttcgagtg
     3961 atttgatgta gtcgtaaatc gccaactgcg cccgtacttt gcggcgattg ccgcggggaa
     4021 cgtagcgatt actgagttct ttatcgatat aacgtgcttt gaccgtatcg ctgaacaata
     4081 tgtcgatgat gtccagcacc cgctgcttca ggcgcggatc gagcagcggc gtcgccactt
     4141 caatacgata atcaatattg cgcgtcatcc agtcggcgga agaaaggtag acctttttat
     4201 cgccgccatt ttcaaaaata taaacccggt catgttcaag gtaacggtca acaatactga
     4261 tggcacgaat gttgtcgcta atgccttcca gattggggat cagcgaacac attccgcgaa
     4321 ccagcagatt aaccggtacg ccggagctgg aggccgcata cagacgatca accaggcctt
     4381 tatcgacaag gttatttagc ttcagggtga taccgctggg cagcccttgc tgcgcgttgg
     4441 cgatttcgcg gtctaccatt tcatataaca gacggcgaga gttttgcggt gacaccatta
     4501 aataatcaaa tgtcaccggg cggtatgggt tttcaataaa gttaaatacc cgccgtactt
     4561 cgttggtgat acgcgcatcg gcggtcagca atgaatagtc agtataaaga cgcgcggttt
     4621 tttcgttaaa gttcccggtc ccgatgtgtg cgtaacgcac cacttcaccg ttttcttttc
     4681 gtgaaatcag gaacagtttg gcgtgaattt tcagccccgg cgcagagaag ataacgtgca
     4741 cgcctgcttc ggtcaggcgc ttcgcccagt gaatgttagc ttcttcgtcg aaacgcgcct
     4801 gtaactcaac caccacggtg actttcttac cgttatgtgc ggcgtggatc atcgagtcga
     4861 tgatgcgtga atctttcgcc acgcggtaaa tgttaatttt aatcgccagt acgctcgggt
     4921 cgaacgaagc ctgacgcagc agttccagca catgctcaaa ggtgtgataa ggataataga
     4981 gcaacacatc gcgttcgcga atggcatcaa aaccattgcg gaactgggct ttatcaaacc
     5041 aaatatggcg taaacgcggc agtggtttgt tcaccagatt ggctttgccg acattgggga
     5101 aattaataaa gtctttaaaa ttatgataac gaccgccggg gacgatggag tcgtagcggg
     5161 aaatagtcag tttttcgcgt aacacttcaa ccagcgcatt gggcatatcg cgctgataaa
     5221 caaaacgcac cggctcagca gttaaacgct gcttgagact ggaagacatc aactccatca
     5281 ggctggcttc catctcatgc actaaatcgt attcggcatc gcgggtcatc ttcattgaat
     5341 aggcattcag cgcgtcataa tcaaagaagc ctttgaaaat atcatcaagg cagtaacgca
     5401 gaatgttatc cagaagaatc atcggcttgc gtcgacgcgg cgcttctggc ggtaaattca
     5461 caaagcgcgg cactttatct gatgggatct ccagcagcgc gtaacggatg gtatcgccac
     5521 ggataatttc caccgccaga taggtgtaat catctttcag gaactgcact aagtcagtgt
     5581 caggattgat taaaatcggc gtaatgtgct gacgcagata ctgcttaaaa taatgacgca
     5641 gccagttttg ttgattgacg gagagctggc gttcattaat caggaagatc tggttacgcg
     5701 ccatctccag cagcagctcg ttgtagaggc cgtcgaattc ctgatcggct ttcagcaccc
     5761 gggactgaat tttgcccagt aaatggcggg aatgagagtt ggagccttgt tcttcgctaa
     5821 taatgatgcg tcgcttcagt tcagcgaagc ggactttata gaactcatca aggttattgg
     5881 aatagatccc caggaaacgc atcctttcaa tcagcgggtt agatttgtcc gccgcttcct
     5941 gaagcacgcg ttcattgaac gataaccaac tgagctcttt ttcgatgtat agcttttcct
     6001 gacccattac cacttttact ccgttttatt cacgggacac tgcctggata ttatggcgag
     6061 caatattggc agatgtccaa ctatgccaga aaagggggaa aaaaactgct ggagcttgcg
     6121 atttacaggt gaattgaaaa acgccagcgg cagagctggc gctttaacta cggggcatta
     6181 ctcgtcggca gcgtagccct gcggcggcag acgttgacca tccagccacg cggcgttttc
     6241 gtgcattttc agacgaccat cggcaaacca gctaatcacc agtggataaa tagcgtgttc
     6301 ctgggtttgc acgcgggcgg tgatgtcatc ttccgtatca ccagcaaata ccgggacttt
     6361 cgcctgtaaa ataaccgggc caccgtccag ttcatcggtg acgaaatgca ccgatgtacc
     6421 gtgctcttca tcaccatttt ccagcgcctg acgatgggtg tgtaatccgg gatatttcgg
     6481 cagcagagaa gggtgaatgt tcagcaaacg cccggcatag tgggagacaa acgccgggct
     6541 gagaatgcgc ataaaaccag ccagcacgac cacatcgggt gcgtacatgt cgatttcatg
     6601 aatcaactcc cggtcatagg cctcacgact gtcaaacgcg ctggcgatga gcgtatgcgt
     6661 tgcaataccc gcctggcggg cgcgttcaag gccgaacgcg tcggccttat tgctgaaaac
     6721 tgcccgtacg gtgcctttaa ttttgttggt tttacaggcg tcaataattg cctgtaaatt
     6781 acttccgttg ccggaaataa gcaccacaat attcattatt cgataaccac gcgttgttcg
     6841 gaatcagagg ctttgatgat accgattttc cacgcgtttt caccgttggc attgagtagg
     6901 gcgagggctt tgtccacttc cggagcaggc agggcaataa tcatcccgac gccgcagttg
     6961 aaggtgcgat acatttcatg gcgctcaacg ttacctgccg tttgcagcca gttgaacact
     7021 tccggccact gccaggaaga ttcatcaatc actgcctggg tattatctgg caatacgcgc
     7081 ggaatgtttt cccagaagcc gccgccggtc aggtgcgcaa tggcatgcac atcgaccttt
     7141 tcaatcaact ccagcactga cttcacgtaa atgcgggtcg gtgccagcag atgatcggct
     7201 aatggcttac catcaagttc ggtggtttgc ggatcacaac cgctgacttc aagaattttg
     7261 cgcaccagcg aatagccgtt cgagtgcgga ccgctggaac cgagtgcaat cagcacatcg
     7321 ccgtcgctga ctttagagcc gtcgatgatt tctgattttt ctaccacgcc aacgcagaaa
     7381 cccgcgacat cgtaatcttc accgtgatac atccccggca tttctgccgt ttcgccaccc
     7441 accagtgaac agcccgattg cagacatcct tccgcaatgc cgctgatcac cgctgaagcg
     7501 gtatcaacat ccagttttcc ggttgcgtaa tagtcgagga aaaacagcgg ttctgcacct
     7561 tgcaccacca ggtcattaac gcacatggcg accagatcaa taccaatggt gtcgtgacgt
     7621 tttaagtcca ttgccagacg cagcttggta cctacgccgt cagtgccgga aaccagcacg
     7681 ggttcacgat atttttgcgg caatgcacac agcgcaccga agccgcccag accgcccatc
     7741 acttccggac gacgcgtttt cttcactacg cctttgattc ttccaaccag agcattaccc
     7801 gcgtcaatat caacaccggc atctttgtag ctaagagagg ttttatcggt cactgcttgg
     7861 gtccccacgc gttacttgcg gtagaaaaat aaaattcggc gcaattctaa cagggaaagc
     7921 aaacgtttgc gagactgctt tacacaacct ttttgcacgt cttttcccca gacgcgcggc
     7981 gaaagaagac ttgtgccagg gtaaaggtta gttttcggat ggaataatct tctttcataa
     8041 ccatttgaat ataaaataac tttatctcaa accgttatca ttttgactaa agtcaacgaa
     8101 aagaatattg ccgccttgaa gaaaggaggt ataatccgtc gatttttttt gtggctgccc
     8161 ctcaaaggag aaagagtatg aagatcgtgg aagtcaaaca cccactcgtc aaacacaagc
     8221 tgggactgat gcgtgagcaa gatatcagca ccaagcgctt tcgcgaactc gcttccgaag
     8281 tgggtagcct gctgacttac gaagcgaccg ccgacctcga aacggaaaaa gtaactatcg
     8341 aaggctggaa cggcccggta gaaatcgacc agatcaaagg taagaaaatt actgttgtgc
     8401 caattctgcg tgcgggtctt ggtatgatgg acggtgtgct ggaaaacgtt ccgagcgcgc
     8461 gcatcagcgt tgtcggtatg taccgtaatg aagaaacgct ggagccggta ccgtacttcc
     8521 agaaactggt ttctaacatc gatgagcgta tggcgctgat cgttgaccca atgctggcaa
     8581 ccggtggttc cgttatcgcg accatcgacc tgctgaaaaa agcgggctgc agcagcatca
     8641 aagttctggt gctggtcgct gcgccagaag gtatcgctgc gctggaaaaa gcgcacccgg
     8701 acgtcgaact gtataccgca tcgattgatc agggactgaa cgagcacgga tacattattc
     8761 cgggcctcgg cgatgccggt gacaaaatct ttggtacgaa ataaagaata aaaataatta
     8821 aagccgactt taagagtcgg cttttttttg agtaaagcgc ctataacaca taatacagag
     8881 gataatacta tgacgcgccg tgctatcggg gtgagtgaaa gaccgccact tttacagaca
     8941 atcccgctta gtttgcaaca tttgttcgcc atgtttggtg caaccgtcct ggtgcccgtc
     9001 ttatttcata ttaacccggc gactgtactg ttatttaacg gtattggaac gctgctgtat
     9061 ctcttcatct gtaaagggaa aattccggct tatcttggtt ccagctttgc ctttatttca
     9121 ccggtattgt tactgttgcc gttagggtat gaagtcgcgc tgggcggctt tattatgtgc
     9181 ggcgtgctgt tctgcctggt ttcttttatc gtgaagaaag cggggaccgg ctggctggac
     9241 gtgctgtttc caccagcggc aatgggcgca atcgttgccg tcatcggtct ggagctggcg
     9301 ggcgtagctg ccggtatggc gggtttactc ccggctgaag ggcaaacgcc agactccaaa
     9361 accatcatca tctctattac caccctggcg gtcacggttt taggttccgt gctgtttcgt
     9421 ggtttcctgg caattatccc gattttaatt ggcgtgctgg tgggctacgc gctctctttc
     9481 gcaatgggaa ttgtcgatac cacgccgatt attaatgctc actggtttgc gctgccaacc
     9541 ctctatacgc cgcgcttcga gtggtttgcc attctgacta ttctgccagc ggcgttagtg
     9601 gttattgccg aacacgtagg gcacctggta gtaacggcta atatcgtcaa aaaagatctg
     9661 ctgcgcgatc caggtctgca ccgttcgatg tttgctaacg gcctgtcgac cgtgatctcc
     9721 ggcttctttg gctctacgcc aaataccact tacggagaaa acattggcgt gatggcgatc
     9781 acccgtgttt acagtacctg ggttatcggt ggggcggcga ttttcgctat cctgctttcc
     9841 tgtgtcggta aactggctgc cgctatccag atgatcccat tgccggtgat gggcggcgtt
     9901 tcgctgctgc tttatggtgt catcggtgct tccggtattc gtgttttgat cgaatcgaaa
     9961 gtggattaca acaaagcaca gaacctgatc ctgacttccg tgattttgat catcggcgtc
    10021 agtggcgcga aggtaaacat cggcgcggcg gagttaaaag gtatggcgct ggcgaccatc
    10081 gtcggtatcg gcttaagttt gatcttcaaa ctgattagtg tgctgcgccc tgaagaagtg
    10141 gtgctggacg cagaagacgc ggatataaca gacaaataat gtaccgaccg ggcagtgttg
    10201 ctgcccggtt caaacatcat gggattctgt ggtaaacttc tcgcgatttt gtgaaatcct
    10261 ggttgaggta tctctgaaca caccggcaca gctctctttg ccactttatc ttcctgacga
    10321 cgaaaccttt gcaagtttct ggccggggga taactcctct ttactggctg cgctgcaaaa
    10381 cgtgctgcgt caggaacata gcggttacat ctatctctgg gcacgcgaag gcgcggggcg
    10441 cagccatctg ctgcacgcgg cttgcgcgga attgtcgcag cgtggcgatg cggtgggcta
    10501 tgtcccgctg gataaacgca cctggtttgt tccggaagtg ctcgacggta tggagcattt
    10561 gtcgctggtc tgtatcgaca acattgagtg tattgcaggc gatgagttgt gggagatggc
    10621 gattttcgat ctctacaatc gaattctgga atcgggcaaa acacgattgt tgatcaccgg
    10681 cgatcgtcca ccgcggcagt tgaatctggg attaccggat ctcgcgtcgc gactcgactg
    10741 ggggcagatc tacaaattgc agccactttc tgatgaagat aagttgcagg cgctacagtt
    10801 acgcgcgcgt ttgcgtggtt ttgaactgcc ggaagatgtg gggcgtttct tgctgaagcg
    10861 gctcgacaga gaaatgcgca cgctatttat gacgttggat cagttggatc gtgcgtcgat
    10921 taccgcgcaa cgtaagctga ccattccgtt tgtgaaagaa attctgaagt tgtagataag
    10981 gtgtttattg tcggatgcgg cgcgaacgcc ttatccgaac tacgcgaagt taatccatag
    11041 tattcgtagg ccggataaga cgtggcaagc gtcgcatccg gcaatgtgct tcaactagcg
    11101 atggagcaca tcttaaccca cgatttccag tacctgctcc ggtggacgac caatccgcgc
    11161 tttgccattc gcgaccacaa tcgggcgctc cattagcttc ggattatcca ccattgcctg
    11221 aatcagcgcc tcttcgctaa gcgaactgtc agccaggttc agttctttat aaagatcctc
    11281 tttctggcgc atcaattcac gggcgctatt catccccagc attttcagca aatcgcgcag
    11341 cgttgccgca tcggcgggtg tctcaaggta gagcaccact tctggctcca cgccgttttc
    11401 tttcagcagg ttcagcgttt cccggctctt tgaacagcgt gggttatggt agatttttac
    11461 ctgtttggtc attgttcttc ctttaatgcg aattacatct tggtataagg cttaaagcgt
    11521 tcctgcagct ggcgcaactg gtcgatgcgc gcatcgtaac gcgcttgttg caggctgcct
    11581 aatttcacct gcgaactggc gctactcaac agcgaaatgg cctgatcgag tcgtccggcg
    11641 agcgcataac cttctgctcg cgcagccagc tcctgatcac ggttatttag cgcggcttcc
    11701 gcctgtgcca gcaaatccca gccgttgctg tcatctttat tattaaaggt gtagcgattc
    11761 agaatattgg ccgcttcttg tggttgaccg ccttgcagat aagcgttcgc caggttgagc
    11821 tgcaacaccg gattggtgcg taaatcgcgg gcatttttca ggcgattgat cgcttcattg
    11881 gctttgtttt gcccaagatc gatatcagta gccagatcga gataccatgc gttgccaggt
    11941 tctgccgcca gtaacggttg cagcgtttta cgcgcctcgt cgtatttatt ggcttccatc
    12001 gcctgtaaag cacgaccata ttgcgctgct cgttgctgac gaacgtttcc tttcgcccat
    12061 tcatccagca aatcactggt gagctggtta cgtccggaat tatacatccc cagtgtgcgc
    12121 gctttcgcca gatagaaatc ttccgacgac tgcaccacca tcgggcgcat ctgattagca
    12181 cggttgcggg catctgccag acgactttcc ggcaacgggt gagtcaataa aatttccggc
    12241 gggcgcgagg agtaacgcgc ctgatcgagt aatttttcga ggaaggttgg catcgcctgc
    12301 ggatcgaatc ccgagcgttg cagcacctga ataccaatgc ggtccgcttc ctgttcattt
    12361 tgctgggtga aactgatcat cccctgacgc gttcccgcca gtgtaccggt cagcgccgcc
    12421 atccccgcct gcggactggc catcgccagt aaaatagaac ctaacgcgcc gacccaggtc
    12481 agcggcgcgc tgcgctgctg atcttccatc gctcgcgcca ggtgacgttg ggtgacgtgg
    12541 gagatttcgt gcgccataac tgaagccagt tgactttcgt tatcggaata acggaacagg
    12601 gcagagtgca gcaccacgtt gccgccaaag aaagcaaagg cgttaatttc gtcgttgttg
    12661 atcagaaaaa aatgaaacgg tgtcttaacc gaattggcat gcgaaaccag acgcatcccc
    12721 agcgaattaa tatattgcgt taacagcggg tcattaatta acggcgcgct gccgcgtagc
    12781 tggcggacat aatagtcgcc catctgcatt tcctgaccaa tggaaagcgt gcttcctgcg
    12841 gaggttccca tatccggcaa ggtgtctgcg ctgtcggcaa acgccggggc tacctgacca
    12901 atggtcattg cagcaatgag ggttgcaacc aggttttttt tcaactgcct gaacataacc
    12961 tctatcctgt atttctgaag atagtcattt gaccgatact gtgaaatatc gttcccaccg
    13021 cgccgccttt gagtgtaacg gcgaagatta gcccactcaa atgacttttc agtttcctga
    13081 atccgtcata aaaagcgaag tcttttttgt gacatttcga tacaattgcg gatcgcaatc
    13141 ccgctcttga ggttcaggga aggtctttat gctcgaaatg ttgatgcaat ggtatcgccg
    13201 ccgttttagc gacccggaag cgattgcctt gctggttatt ttagttgccg gatttggcat
    13261 tatctttttc tttagtggcc tgcttgctcc gttgctggtg gctattgtgc tggcctattt
    13321 gctggaatgg ccaaccgtgc gcctgcaatc tattggctgc tcccgccgct gggcgacgtc
    13381 gattgtattg gtggttttcg tcggtatatt gctactgatg gcgttcgtgg tactgcctat
    13441 cgcctggcaa cagggcatct acttaatccg cgatatgccg gggatgctca ataagctttc
    13501 tgactttgcc gccacgttgc cgcgccgcta tccggcgtta atggatgcgg gcattattga
    13561 tgcaatggcc gaaaatatgc gcagtcggat gctgactatg ggcgattcgg tggtgaaaat
    13621 ttccctcgcc tcgctggtcg gtttgctgac catagccgtc tatctggtgc tggtgccatt
    13681 gatggtcttc ttcctgctga aagacaaaga gcagatgctg aacgccgttc gccgggtgct
    13741 gccgcgtaac cgtggactgg caggacaggt gtggaaggag atgaatcaac aaatcaccaa
    13801 ctatatccgc ggcaaagtgc tggagatgat cgtagtaggg atcgccacct ggctggggtt
    13861 cttgctcttc gggctgaact attcgctgct gctggcggtg ctggtcggct tctcggttct
    13921 tattccgtac attggcgcat ttgtggtgac cattccggtg gttggcgtgg cgctattcca
    13981 gtttggcgca ggtactgaat tctggagctg cttcgcggtg tatctgatta ttcaggcgct
    14041 ggacggcaac ctgttagtac cggtgttgtt ctccgaagcg gttaacctgc atccgctggt
    14101 gattatttta tcagtagtga tcttcggtgg tttgtgggga ttctggggcg tattcttcgc
    14161 cattccattg gcgacgctga tcaaagccgt gatccacgcc tggcccgatg ggcaaatcgc
    14221 gcaagaataa tcttcagccc gacgaactgt ttcgccgggc tgatttttca ggattcctga
    14281 cgtaaataaa tagcccgata acaaatactc accagcactg caccgccgat aatattaccc
    14341 agcatcaccg ggagcagatt ggcggtaata aaatggctga ccgtcagtgc cggaaaattg
    14401 tctgcgctac tgtgcgccag ttgccgaagg ggcgtggggc aaaatggcga atggcaatgg
    14461 cgaacggaat cacaaacaaa ttggcgatac agtgctcaaa gccactggcg acaaacaggg
    14521 tgatgggcaa aatcatagcg acgattttgt cgcataatga acgcccgcaa taactcatcc
    14581 acagcgccag gcaaaccatc agattgcaca taatgccgag gctgacagat tcagtaaatg
    14641 tatgatgcat tttgccctcg gcgcagtgta aaaccgcgac tccccacatc gcgttttcac
    14701 tcatcaccag cccggaaaac cagattaaca aactgaaaca ggcaatacct gccagattac
    14761 cgcaggccac cagaagtgcg ttaatcagcc aagttcgcca actaataacg ccccgacttt
    14821 ttgccatcac cgtcattacc gacgaggtga acaggctggt gccacaaacc gccagcaaaa
    14881 taaagccgag tgtaaagcaa aggccgccca ccagatgggt taatgcctgt gaagacgggg
    14941 catcggcgat aacggaaagg taaaaagtaa atccgatcgc cataaacacg ccagcggaca
    15001 tcgccattaa aaagaccgac actttactgc gggcaatttt atgggaggca atccgttcag
    15061 cgattgccgc ttttctggcg ctcaactgca agtcgaaaga gagtttgttt ctcatgcata
    15121 ctcctgcaaa agcagattac aacacctcgc gaaccgagat ccccagacgc tgcattcgtg
    15181 acagcagcgt ggtgcgcttc atccctaatc gtgtcgccgc gccacggggc ccggcaacaa
    15241 tgccattggt ttctcgcaat acctgaacaa tgcgctgacg ttcttcttcg tcattctctg
    15301 gcgtcgtcgg gtgcagcaac tgcgccattg aactgcgaag cgctgaatct tcatttagcg
    15361 tcggcagtaa acggctttgt cggacattta gatgtaaatt cagattgtta ccacgagtca
    15421 acagtaccgc ccgctcaatc acgttttcca gctcgcgcac gttgcccggc caatcccacg
    15481 acatcaactg gcgtagcgcc tcggtcggga ttgcgtcaat tgcgcgattc atatggcgcg
    15541 ccattttttg cgtgaaatgc tttgctaaaa gagggatatc ttccggacga tcgcgtagcg
    15601 gcggcaattc cagtgggaag acattcaggc gataaaagag atcgctgcga aactggcgat
    15661 cttcaaccat ttgccacaaa tcacggttgg tggcggcaat gactctgaca ttcaccggga
    15721 tcgttctact cccgccgaga cgctcaatct cccgctcctg caatacgcgc agcaatttag
    15781 gctgaagttc taacggcaga tcgccaattt catcgagaaa caacgtaccg ccatcggcaa
    15841 tttcaaaacg accacgatgg gtattaatcg caccagtaaa cgcccctttg tcatgaccga
    15901 ataactcact ttccagaaga ctggcgggga tggcagcgca gttgattttg accagcggct
    15961 tgtcgcgtcg cgggctaagt tgatggatcg ctctggcgat cacctctttg ccagttccgg
    16021 tttcaccgca aatcaacacc gtactgtcgc tcttcgccac aatatctacc tgctggagca
    16081 ggtcttccat tgcctggctt tgatagataa tgtcaccgac gcccagattc gaaaggagct
    16141 gctcgctaag ctggtggttt tcttgctgca aactttcctg caaatcggtc atgctacgcc
    16201 aggcatcggc attgccaacg gcaatagcga tgcgatcggc tatgtgttgt agtagctggc
    16261 agttttcctc actaaagaga gtggaagagg tatgcgccag taacaatgca cccggtgtat
    16321 ggttgccaaa ggtaagcggt atcaggagcg cagattcgca gccgttagat gcaagtaaga
    16381 gtaataacgg gtcgcgttgc cagagaaaca gatcgtctgc acggtgcgtc aaggtcggtt
    16441 gtcgggtttg tagcgtttgt gtcagcaata cactgtcgcc aggcatattg cgtggcagac
    16501 acgcacaatg tgaggcagaa agatcgctgc accacaggct gaacttctcg ttctttcgat
    16561 gatcgcccag taccatactg actgaagcca gaccgaaaaa atgatggatc tcacgagcga
    16621 cgtcagcgat cagatcgtcg agatcaagat gtgacagcac tgcattagtg atatccacca
    16681 gtacccgatg atgatcgcga tctttctcca gcaaacgcag cgccacctgt tgttcttcat
    16741 cattgagcca tgattgcaga actggggtga taatgcgtgc cagccagtta aacagtgtgc
    16801 aatcctgggg agtaaaacgg ctgccgtggg tacgaataaa gcgtagctcc gccattccac
    16861 tactgccgag aaggacggcg tgacgcgaat aatcgaccat ctcgccctga tactctactg
    16921 ttgccgtcac atcgctgcca tcatcatggt agcggtaata gaagttgctt ttgagaacat
    16981 gcagttcaat aagttgtacc aggctgaacg gcggcggcag cgggatgaag gcgcgtaata
    17041 aagacgcctt gccgtgtttc tgtgctaacc gctccgcgag cattccgttt accgcttcaa
    17101 ttgttattcc ttgtggcggg gcaaacatcg cctcgtctga catagccatc aacagtcctt
    17161 attaatgaga aatccttctc acgcaatttt tctggccatc agatagatgg ccggttcctg
    17221 cacaatttca tccaacagat gaatgaattg aatgctacgc gcctgccaca cagacgtcgc
    17281 gtcatctgcc agtagcgcga atgcccgggt caacagggcg gtgtggctgg cgtcaataac
    17341 aatctcacca aaggtcatca cccccagcat cttacgtcgg gcttgctcct cttcgacctg
    17401 tgcaagccaa tcttcgtatt ccgtcagtgg gcagcggaag gcgacattca gacaatcaat
    17461 cacgccaacg tgatgaccga tagccagcga gtaatacatt acctgagagc tgtgttccgg
    17521 catctcgtcg ctactggcga caaacttttt tcgcagcgtc cagataacaa tttccccgca
    17581 ctcctcagcc atgtaacccc cggagaacgc gaaagagttg ctgaacgata ctattgagac
    17641 gcggatcgtc ggcttcgcgc aaccaggtgt taacccgatt tcctgtagga tcgtcgctta
    17701 aatggcgcag gagccgatcg caaatttctc gcccctgacg atagcccgcc agccgccgcg
    17761 cttctcgctc aatggcgata cgctgtgatg gcgggatctg tggccacaac ggttgcatag
    17821 tcaccccggt gggatcgcga taatccacag cgtgaatctt ctgttgcagc aaaccgagcg
    17881 ccacggcgaa accgtgaatg gtggcggccg gtgttggcgg gcagccgggg atccaaacat
    17941 caatggggac aatggtgtcg ctgccgcccc agacgctgta gagatcgtgg aaaataccgc
    18001 cgccgacacc gcacgcgccg taggaaacac agattttatg atcgggggca gactcatacg
    18061 cccgaagtgc aggcatacgc atcgcccggg tgactgcgcc tgtaaataac aaaatatcgg
    18121 cgtgacgcgg tgatgaaata accttaatgc caaaacgttc tgcgtcgaat actggtgtga
    18181 tggcggcaaa aatttcgatt tcacaggcgt tgcagccgcc gcaatcgacg cgataaacgt
    18241 aagccgagcg acggatatcc tgtagcaaat gctgcttcat cttttgcgtt tgctcgtcca
    18301 gcgtgatggg ctggctgaca tgttgtgtaa gcactggact catagctgct ccttagccac
    18361 cagcggtaca tctgtatcgt cgttgagcag cgtcgcgcgt tgtttgcact ccgggcagac
    18421 gctcgcttgc gcccataaca tttcgcggtt ttgtggcgca ttttgttgct gtgccaacaa
    18481 ttcagcagcc agtgcgacgg ttttttgcgg ggcaaacggg cgttcgcaac ggctgcaacg
    18541 ttgtagatgg aacgtcgcgc gggtatagag atcggcttta ttggtgacgg tcagttcaaa
    18601 gttattggta agctggatgg ctctggtcgg gcacacttct tcacaacgtc cgcagtaaat
    18661 acaacgcctc agatagagct gccaggtgtg cgaattttgc tggtcgtcgg tctggatagt
    18721 cagcgcattt gccggacaag cacaggcgca ggcaccgcag gcaatacatt ggctgggcat
    18781 caggtccggt ttcccgcgaa agcctgggct gacctccagt ggcgcgaagg gatatttcac
    18841 cgtcgcggtt ccggcgcgca taatagtttt cagtaacttc agcatcttct gtccttactt
    18901 cagcggcgaa cggttacgat caataccgta gcgttcgatc tctttatacg gcacggtttt
    18961 tgactggcgc ttgcgcacat ctaccagcgt cacacggtcg gtacaggagt agcagggatc
    19021 aaggctaccg ataatcagcg gtgcgtcaga aacggtattg ccgcgcagca tgtaacgcaa
    19081 caccggccag ttggcgtagg tggcggcacg acagcgccag cggaacaatt tttggttatc
    19141 accgagcatg ctccagtgca cgtcttcacc gcgtggcgct tcaacaaagc ccagcgcgaa
    19201 tgcgtgaggt ttatagctaa agccttcggt cagcagcggg gtatccggca tgttgtcgag
    19261 ggcaaattcc agcattgcca gcgaatcaaa cgtctctttg acacggacca tcacgcggga
    19321 gaaaacatcg ccgccggtga aggtaaacag tgtttttgga atattgccgt agtcggcgta
    19381 ggggtgatca aagcgcaaat cacgggcaaa accactgccg cggatcagcg gccctacagg
    19441 gctataatca cgggcgattt gtcggtcgag aatgccaatg ccctgagtgc gttgttccat
    19501 attcggcata gcaagcagca tctctaccag ctccgacacg tcggcgcgca tctcgcgcac
    19561 cagtttcagc gtttgcagac gttgctcttt gagaatatcg cggcgaacac caccaatcag
    19621 attcagaccg taggttttac gcgacccggt cagcaattcc gccatcgtca tcgacttttc
    19681 ccgcacgcgg aaaaattgca taaagccggt atcaaaacca acaaaatggc aggagaggcc
    19741 aaggttgagc aaatgactgt gtagccgttc gacttccagc agaatcgagc gaatggtatg
    19801 cgctcgttgc ggcacctcaa tccccagtgc attttcaacc gagttggtat aggccacact
    19861 gtgggcaaaa ccgcaaatcc cacacacgcg gtccgataag aaggtcactt cgttgtagcc
    19921 catccgcgtt tctgccagtt tctccatgcc gcgatggaca taaaacaggc ggtaatcagc
    19981 atcgacaatt tgctcgccat ccacaaacaa gcggaagtga cccggttcat cggaggtgat
    20041 atgcagcggg ccgacaggga tcacccgcgc accgctgttg ccctcattga taaacgcata
    20101 cgtttcggaa tctgtcgtcg gttcagggcg cagtcgataa tccatcgcat ctttgcgcag
    20161 cggatgcata tcttccggcc agtcatcggg caacaccagg cgacgctgat ccggcaggcc
    20221 aaccggaatc agcccgtaca tatcgcgaat ttctcgctcg ccccagaccg cggcagggac
    20281 gcgcggtgtg actgacggaa actcccgact gtcggcatcg accagtgcct tcaccacgat
    20341 ccagcatttt tcggcccctt ccattgaaag ggcataataa accgcgtaat gaccgttaag
    20401 tgtccgctcg tcgttgccaa acaggactgg aagccagcca tcatgttgat aataaagata
    20461 atgtacaacg tcaggcagca gattgatttt caccgtaatg gtgacctgtt caggcgtttg
    20521 tcgctcttca tccagcaccg cgccggggaa ctgcgttttc agggcggcga gaatcgcttc
    20581 gccacgattt gacgatgaat taacgttcac tgtgtttctc cgaaacagat gcggtgcctg
    20641 aaggcaaaaa atcatgccag gtgctacgtt gtgcaggcag gtcgtgcgtc cctgagagga
    20701 ctatagtgga agcgcccgcc aggatcctga tgacaggttg tggaatatgc gttcccatcg
    20761 ccagcatcat gaccagcaga atcaccattg gcgaggtggt caaccagccg agatcacccc
    20821 ggttaacggc ctgcggcggt ttcgccatta acacccgcgc tgccatccgt accaggcccg
    20881 ccagcaccag cgttaacagc aataacagca ggacgataat cagcaggtga ttacgtgcca
    20941 gtccggcggt aatggtcata aattcgctaa gaaaaatgtt gaagggcggc atccctgcca
    21001 gcgccagcgc cagcgcaccg ccgccaaaca gcacggcggt aaatggcatg attttgagca
    21061 tcccacagac gacgttgaga tcgcgcgtgc cgtacttgag cagtacattg ccggaaccgc
    21121 agaacagcag cgtttttgcc agactgtggt ttaaggtgtg cagcagcgcg gcaaaaattc
    21181 ccagtggccc gccaatgcct agcgccaccg cgaccagtcc catgttctcc acgctggagt
    21241 acgccagcag acgcttaatg tcccgctgta ccagaatgaa aaatgccgcc acggcaaccg
    21301 acaacatgcc gaagatgagc aacaaccggt tggggaaatc gctgccgatg gcctggcaga
    21361 taatgatgta atagcgaatt agcaccaaca gcgcgcagtt cagcaatacg gcggagagca
    21421 gggcgctgac cggactcggt gcttcactgt gagcatccgg cagccaggcg tgcatgggaa
    21481 atagcccggt tttggtgcca aagccaatta gcacaaacac aaaggccaac agcattaatg
    21541 ttgggtcaag caaggacgat tgcttaagaa cctcgctcca gaatatcgcc atttctgcct
    21601 gcggcataac gctggcgacg ttggcgtata ccagcacggt accgaacaga ccaaaagcga
    21661 caccaacagt acaaataatg atgtacttcc atgctgcttc cagcgatgaa cgctgaccgt
    21721 aaatgcctac cagaaacgcc gagcttaagg tggtggcttc gatcgccgcc cacatcacaa
    21781 tcaggttatt gctggtaaca accagcagca tggtaaacta aaacagatgg aagaaaccgt
    21841 agtaatcgca cagcgtaacg ggggaaagct cgccgtgtgc cacttcatga cgcatgtagc
    21901 caatcgagta aacaccggtg agaaagccaa tcacaccaag aatggcgagg aacaaaccgc
    21961 cagaccatca atatgaagcc acagtcccgc agcgaatatt tctcctgcat cagcggcagt
    22021 ttggaccacc cagagtgcca gaatcagcag cagtgtgatc cctaagctat gtaatacagt
    22081 caccgtacaa gtcgcagaga gcccccgttt ccggcaggca aaacagagca gcgaaaaaag
    22141 cagcggcgtg agcaggagta aagcgaacat cacagaataa ctcatctcat tatcccttca
    22201 gcgcggtcaa gttgttcacg tccagcgtgc cgtgggtacg ccatattttt cttgccagta
    22261 acaccatcac aatgacggcg aagatggcgt cggtagcgat acctatttcc accagttccg
    22321 gtgctcgcca ggcaagaagc gccagcacca gatgggagcc gttttccatc aggcagtaac
    22381 caaaaatttg ccgcaggata ttgcgctggc tgacaatgca cagcaggcca agcagaaaat
    22441 gacctaacgc taccgccagc gccggtttca gcccggtagc catcggtagc ttcacgggct
    22501 gaacgacaaa tgcgcaaagc aggacaatta acgccgcgag cagagccatc attgccggac
    22561 cgaataacgc tttttccggg atgttctggg gaatatttcg tgcagcgtaa gtcatgatta
    22621 acggtaccag cagcactttg gtgataaagg cactggcgga ccagatcagc agttgctctg
    22681 cggcgaacag gcacgagaga gtggcgaaaa tagacaccag caccagcgac tggcaggcgt
    22741 aaaatccgca tgacaggcga tagcttttga cgctaatcac aaacagcgat gtcagcatca
    22801 tcagtcccgc cagattattt acgatcatag aaccggtcat tcatcatctc cttactgcaa
    22861 ccaggtcgcg gcgattacgc tggaaatcag cgacatcaca atcagtacaa tcaacaccag
    22921 gcgcattgac cctggcagcg gtgcggcatc ggcgacggcc tcgctcggtt tgccaggcac
    22981 aacgcgacca aaccagcgaa taaaccaggc gaaactggcg accgattcaa tcatcagcag
    23041 aatcatggcg ggcagcagga tccagtactc cactgacaac gcaaaaccgg cagcaaacag
    23101 cgggaattta ctgaagaagc cgttgaacgg cggcacgccg gtaatcgcca gcgctgccac
    23161 gcagaaaccc acgcctggca acggcagggt gtgcaatacg ccacgcagac gcggcaacaa
    23221 gcgcgtgccg cagctgtaac tcagcgcacc tgctacaagg aaaaacaggc ttttagcgaa
    23281 cgcgtggttg acgatgtagg cgatgctgcc ctccagtgcc agccgcccac tttcaccatc
    23341 gatgcggcgt ggagataggc gctgatcggt gttggcgctt ccattgcgtc cggtagccac
    23401 gcttgcatcg gtagctgggc cgatttcccc cacgcggcaa acaggatgcc gccataaacc
    23461 agataacgtg cgtcgccatg taactcgctc atcgcgctaa gcacaaacgt tccggtctgc
    23521 aaaaacagcg tggcggcggc aagatacaac cccaacgaac cgatatgggt gataagcaac
    23581 gcttttagtg ctgaacgctg cgctttatcg ctctggtaat aactgatcaa cgcccaggag
    23641 cagccgcccg taatttcaaa aaacaacaac tgaccgagca gcgtcgagga gagtaccagt
    23701 cccgccatcg cgccgataaa caacagcaaa atgcgtaata acgattcgtg ccgttatgcg
    23761 ggtgttcgcg atttttatcc gtcagataac ccgtggagta gatcgtgacc agcaaaccga
    23821 gaaacaccac cacaaacaga atcagcgtac tcacgcgatc aatgaccagg ccaaacaatg
    23881 ccacatcccc cacgttgacc aacgtcaacg tgacggcaac tttatcggcc tgataaaacg
    23941 cggagatcag cgacaacatg cacagcgtgg tcagtgcggc gaacaaaacc cccattcggc
    24001 ggcccgacgt tgtggcgaaa acgaaacgac cagtgcgccg ataaaaggca gcattaacgt
    24061 cgtcagagca agattttcca tattccgtca gtgctcctta cagaccggtt aaccagaaga
    24121 cccatgcaag cgcagcaagg ctgaagccaa gccaggtcac atggtgaatg agtaaaaaac
    24181 gcccgcgtgc cagcgtgttt tctgcgattg aggccagtac aaaaatcagc aaaaccttga
    24241 gcagcgtaac gacaagtgaa gtcagcaggc aggcgagaga aagttcttgc gcgcgcccaa
    24301 agggcagaaa cagggccaca aacagtgctg ccatcacgac ctgtttcagc cccagccccc
    24361 atttcgctag cgccagcccg gcaccggaat attcggtcag cgggccttcc tgtaattcct
    24421 gttctgcttc agcaacatca aagggaattt ttcccatctc aatgaagcag gcaaaaccac
    24481 aggccagtaa cgccagtacg gtggttagcg gcgagttcca gcccgttgcc agcgtattgc
    24541 tgatcatctc gatatgcgtg gaacctgcta tcagcgccaa taccagcagt gagagaatga
    24601 gcatgggttc gaccagaatg ccgagcgtca actcgcgact ggcaccgact cccgcaaacg
    24661 ggcttccggt atccagcccg gaaagagcaa agaaaaaacg aaacagggca agaagataga
    24721 taagggtgat cagatcgccg ccgcccgcaa aaggggaaac ggtaataaaa agtggtaggg
    24781 ccatcgccag caccagcatg ctgctgatta atacccacgg catcaggcgg aacatcagac
    24841 ctgaagatat aggcgcaact tcctggcgtt taaacagttt gtggatatcg cgataatcct
    24901 gccagatccc cggcccgcgg cgggagtgca tacgcgcgcg tatctgccgg gaaatacccg
    24961 taaaaagcgg ggttagcatc agcagaatca cggcctgtgc taacgcaaaa atgaccagat
    25021 atccgtcgta aagagtttgt ctcatggtga tttccttaga cggcaatagc gattagcagc
    25081 acaaccagtg cggcgaccac atacaggcaa tagagacgaa agtcgccgct ttgcagatgc
    25141 tgtatttctt tggccagccg ttgggtggcg ctcacgatgg ggcggatcac ccgctcatcc
    25201 cagaagggtt ctgtgctctg agccctggcg gtgacgtgcg caaaaccttt atttagcctc
    25261 agcgtagggt cgagctgttg tcgtagacga aataacgcag agaagaccac acgcagcggc
    25321 tgcatcacgc cattgcctga cggggccatc gcattttccc agccgtagcc gcatgcccag
    25381 gcatctcctg tgcgacgaaa cgctgcgcgg cgcgaacgac accacagcca gtaaaggcca
    25441 ggcagcaaag gcatcgccag tagtaacagc aacagtaatg agggggtgac ctgtgtatga
    25501 aacgtgccgg gtacaagtgc tattccgctg gcgacagtga cgggaggggt attggtaaac
    25561 gcatgggcaa tatgcattat cttcggtgcc agccaactgg cactaagcgc aattaatacg
    25621 cagagtgccg cgagcagtag catggcgacg atcatcgtat ttggcacttc ctgtgcctct
    25681 tcagcgtgtg tactgcgcgg cgcaccacag aaagtaatac cgtacatttt tacgaagcac
    25741 attactgcca gcccaccagt gactgccagc attaccatag caataggacc cgcaagttgt
    25801 agcgctacgg cttccacacg acttagtgag aacagcgact gccaggtgta ccattcgctg
    25861 ataaaaccat tcagcggagg aatggctgat atcgcgaggc aaccaatcag gcatgctgcg
    25921 gctgtccacg gcatccgttt cgccagtgcc cccatttttt ccatgtcgtg ggtatgcaaa
    25981 cgcgaaataa tcgcgcccgc gccgagaaat agcagccctt tgaacagcgc atggttgagc
    26041 agatgaaaca gtgcgccgag cagtccaacc acggtgagca gcgggtcgtg cagtgacaga
    26101 ccgaccatcg ccacaccgac tgccagcaaa ataatgccga cgttttcgac ggtactccag
    26161 gccagcagcc gtttgatatc ctgttccgcc agtgcataca gcacgcccag gagcgcggag
    26221 attgcgccga tcgccatcac cagagtgccc caccacagag gcaaacccgt ttgcgccagc
    26281 agatccatcg ctactttcag gatgccgaaa ataccgattt tgaccattac gccagacatc
    26341 aacgccgaag cgtgcgatgg tgcggcaggg tgagcgcgcg gcaaccagct gtgcaacggc
    26401 atcatcccgg ctttcgcgcc aaaaccgaaa aaggccagca gaaacaccgc cgacgccagc
    26461 cccggagaaa gtgaaagcgt gcgaaactgg caaaatcgag gctgccgctt tcgcgcccca
    26521 tcagcaagaa ggcgatcata atcagcaccg atccggcgtg ggcgataaaa aagtagagca
    26581 tgccagcgtt gatcgacgtt ttatcctgcc tggcaatgac cagaaaccag gaagacagcg
    26641 acatcatttc aaacagcacg atgaaccaaa aagcgttgtc catcaccagc agggcaacca
    26701 tcgatgcgat gaaaagattc ataaagaagc ccatcgccgc cgcgcctttg ccctcgtatt
    26761 cgcgcatata agtcaatgaa tagagcgaac aaaccaccac cagcaaggag ataaccagca
    26821 ccataaatgc cgagaggcta tccattcgca gggttaaatc ggcaaagtca aaaggggtgg
    26881 caaaatgggc gactaaagtt acaccagcat gtaattgcgt aattccgctg atgatgccaa
    26941 tcacgccgcc caccagcgat gtgatgccgg aaagcttaat agccagtctg tccagaccga
    27001 gtaaaaacag cgaagccaga ctggcaaaca gatagaggat cagcgaccag gttaataatt
    27061 gcagggcatc cattagtgtt gctcctgagt gagggaaagg ggatcctccc cgccattggc
    27121 aaaccagctt gcggcaaggc gctgtttttc tttcatctga cgttgcaggc tatcgtcggt
    27181 gatcagccgt aacgcctgat tcgggcaagc gcgaacgcag gccggacctt ctggcaagaa
    27241 atcacacagg tcgcatttca ccgcgacggt ctggacaccc ggttcccagc gcagcaacgc
    27301 atgttggggc agcacgtttt cttcgccgtc tttgagtgag ccttcagcct gaaaaacata
    27361 ttgcgcatgg gcattcaccg gacggcttcc tgaagcgctg attgcgccaa atgggcagac
    27421 cacggcgcaa agcttgcagc caatacagag gctttcgttg agttggatcg catcatccct
    27481 ctgagagatg gcattgaccg ggcagacctg caggcaaggg gcttcctcac agtgatgaca
    27541 cacgacaggg gcagtgattg ttgacgtctt cgccagggcc aggcgcgggt gttgctgtaa
    27601 accttgcgtt ttatgcacgt ccgaacaggc agcgagacag gtattacatc ctgtacacca
    27661 cagtggttcg gccaccacaa agcggttcat gtcggctcct tgaaaatgat atgaaacatt
    27721 gcatttcata ttgcataaag gatgccattc tgattaactc aatatattca ataagttatg
    27781 tattgactgg attctgaacg gtacaaaaat gatggtcatt aacgttcagc aaaaaatgga
    27841 ttcgacaccc gtgtcgcagg ggaaagcgag aatgtgatct tgatcacgag aagtgaggta
    27901 tgctggccgc aagcgcagcc agcacggaat ggagcaaagt aatcaggcgt gttctttcag
    27961 ccagttcagc acaacgtcgt ggtgattgct ggttttgaaa tcgtcaaaga catgttcgat
    28021 tttgccatca gcgtcaatca ggaagctgat gcgatgaatg ccatcgtagg ttttgcccat
    28081 gaaggacttt tcaccccaga cgccgaattg ttcgcacacc tggtggtcct catcagacag
    28141 gagcgtaaag ttaagcagct ctttttccgc aaaacgggag agtttttcgg gtttatcggt
    28201 gctgataccc agcacatcaa cgcccgcttt tttcaactca tccatgttat cgcgtaagcc
    28261 gcaggcctgt acggtacagc cgggggtcat ggctttcggg tagaaataaa ccagaacacg
    28321 ctgtccctgg aagtcggtca aattaacttg ttctccgtct tgatccggca agctaaattt
    28381 cggtgcgata tcaccggctt tcagtggatt cattacttaa ctccatcctg ttcatcatgt
    28441 tgggaataat tgacgacgtt aatactgcct tgtgcattga gttctgtaca tagggctttg
    28501 aacgcttgct caatatttgc tgcgtccgca gatgcggggc tgtgggcggt tatctgaata
    28561 tgcaactgcg cagcccgttc attttcagca ggttgcgtgc gcgacaccag ctccgcaatg
    28621 ttcatatgat gcgcgtcgaa aagtgctgtg aagcgttcaa ttaaatgcgg ggagtctgcc
    28681 acatcgacct gaacccagac agatgctggc attggcggac gcggacgcgc cgtcgtgcgc
    28741 ttcatcacga ttaaaagatc cagttcggca cctttcaacg gtaacgttga ttcaatcaga
    28801 gtaatggcat tccatgaacc ggaaagcagc ataataaacg tgaactcttc tcccagcatc
    28861 gccaggcgac tgtcttcaat attacagccg caactactga catgacgggt gatggtgttc
    28921 acaattccag ggcgatcggc acccaacgca gtgatcacca gataatgttg cgatgacagt
    28981 gtcaaactgg ttattccttt aaggggtgag ttgttcttaa ggaaagcata aaaaaaacat
    29041 gcatacaaca atcagaacgg ttctgtctgc ttgcttttaa tgccatacca aacgtaccat
    29101 tgagacactt gtttgcacag aggatggccc atgttcacgg gaagtattgt cgcgattgtt
    29161 actccgatgg atgaaaaagg taatgtctgt cgggctagct tgaaaaaact gattgattat
    29221 catgtcgcca gcggtacttc ggcgatcgtt tctgttggca ccactggcga gtccgctacc
    29281 ttaaatcatg acgaacatgc tgatgtggtg atgatgacgc tggagctggc tgatgggcgc
    29341 attccggtaa ttgccgggac cggcgctaac gctactgcgg aagccattag cctgacgcag
    29401 cgcttcaatg acagtggtat cgtcggctgc ctgacggtaa ccccttacta caatcgtccg
    29461 tcgcaagaag gtttgtatca gcatttcaaa gccatcgctg agcatactga cctgccgcaa
    29521 attctgtata atgtgccgtc ccgtactggc tgcgatctgc tcccggaaac ggtgggccgt
    29581 ctggcgaaag taaaaaatat tatcggaatc aaagaggcaa cagggaactt aacgcgtgta
    29641 aaccagatca aagagctggt ttcagatgat tttgttctgc tgagcggcga tgatgcgagc
    29701 gcgctggact tcatgcaatt gggcggtcat ggggttattt ccgttacggc taacgtcgca
    29761 gcgcgtgata tggcccagat gtgcaaactg gcagcagaag ggcattttgc cgaggcacgc
    29821 gttattaatc agcgtctgat gccattacac aacaaactat ttgtcgaacc caatccaatc
    29881 ccggtgaaat gggcatgtaa ggaactgggt cttgtggcga ccgatacgct gcgcctgcca
    29941 atgacaccaa tcaccgacag tggtcgtgag acggtcagag cggcgcttaa gcatgccggt
    30001 ttgctgtaaa gtttagggag atttgatggc ttactctgtt caaaagtcgc gcctggcaaa
    30061 ggttgcgggt gtttcgcttg ttttattact cgctgcctgt agttctgact cacgctataa
    30121 gcgtcaggtc agtggtgatg aagcctacct ggaagcggca ccgcttgcgg agcttcatgc
    30181 cccggctgga atgattttgc cggtgacctc cggtgattat gcaatcccgg tgaccaacgg
    30241 tagtggtgct gtcggtaagg cgctggacat tcgtccacca gcccagccgc tggcactggt
    30301 ttctggcgcg cgtacccagt tcacgggcga taccgcttca ttgctggtgg aaaatggtcg
    30361 tggcaatact ctgtggccgc aggtggttag cgtgctgcag gcgaaaaact acaccatcac
    30421 ccaacgtgat gatgctggtc agacactgac caccgattgg gtacaatgga accgtctgga
    30481 cgaagacgag cagtatcgtg gtcgttatca aatctctgtt aagccgcagg gttatcagca
    30541 ggcggttacg gttaaactgc tgaacctgga acaggcgggc aaaccggttg cagacgcggc
    30601 ttccatgcag cgttacagca cggagatgat gaacgttatt tccgccggtc tggataaatc
    30661 tgccactgac gccgcgaacg ctgcgcaaaa tcgtgcctcc accactatgg acgtacaaag
    30721 tgcagctgat gacaccggtt taccgatgct ggtcgtacgc gggccgttca atgtggtttg
    30781 gcaacgtctg ccagcggcgc tggaaaaagt gggcatgaaa gtgaccgaca gcacccgttc
    30841 gcagggtaac atggccgtaa cttataagcc gctgtctgac agcgactggc aggaactggg
    30901 cgcgagcgat ccaggcctgg catccggtga ctataaactg caggttggcg atttagataa
    30961 ccgcagcagc ctacagttca tcgatccgaa aggtcatact ctgactcaga gtcagaacga
    31021 cgcgctggta tctgtcttcc aggctgcgtt tagcaagtaa aaatacaggg ctggaatcat
    31081 ccggcccttt tttctgatat gatacgcaaa cgtgtgcgtc ggcaggaaaa cgcgatttta
    31141 gcggtaattc gcacgaaatt tgtttgtcgg acgtagttcg gataaggcgt tcacgccgca
    31201 tccgacaaaa catccggcac accagacagc aaaagatttt aaaacgttaa ttcacaccca
    31261 ggagtgataa agatgcaaaa gcaagctgag ttgtatcgtg gtaaagcgaa aaccgtatac
    31321 agcacggaaa acccggacct gttggtgctc gaattccgca atgatacgtc agcaggggat
    31381 ggcgcgcgca ttgagcagtt tgatcgcaaa ggtatggtga acaacaagtt caattacttc
    31441 attatgagca aactggctga agcgggtatc ccgactcaaa tggagcgtct gctctccgat
    31501 accgaatgtc tggtgaaaaa gctggatatg gtgccggttg agtgtgtcgt gcgtaaccgt
    31561 gctgctggct ctctggtgaa acgtcttgga atcgaagaag gtattgagct gaacccgccg
    31621 ctgttcgatc tgttcctgaa aaacgacgcc atgcacgatc cgatggtcaa cgaatcttac
    31681 tgcgaaacct ttggctgggt gagcaaagag aacctggcgc gtatgaaaga gctgacctac
    31741 aaagcgaacg acgtgctgaa aaaactgttc gatgatgctg gtctgattct ggtcgacttc
    31801 aagctggagt ttggcctgta caaaggtgaa gtggtactgg gcgatgagtt ctccccggac
    31861 ggcagccgcc tgtgggacaa agaaacactg gagaaaatgg acaaagaccg tttccgccag
    31921 agcctcggtg gcctgatcga agcctatgaa gccgtcgccc gccgcctggg tgtacagctg
    31981 gactgatttt tctggttgct tgtttcttta gccggagggt actccggcta aatgtctttg
    32041 atacccccgc tacaatactt ctcattatct ccctttgaca ggaaagtcat accaggccaa
    32101 tatgtatata cataacgtat atcccaaaaa gggggggcta tggaaactac tgtattttta
    32161 agtaatcgca gtcaggccgt cagattaccg aaagcggtcg ctctgccaga agacgttaag
    32221 aaagtagaaa ttattgcgat tggtcgtaca agaatcatca ctcccgccgg tgagtcctgg
    32281 gacagttggt ttgatggcga aaatgtgagt gcggatttta tggatatccg tgatcaaccc
    32341 gcaatgcagg aaagggaatc attttgatgc tgaaattcat gctggatacc aatatctgta
    32401 ttttcactat taagaataaa ccagtacatg tcagagagcg gtttaacctg aattctggcc
    32461 ggatgtgtat aagttctgta acattgatgg agcttatcta tggggcagaa aaaagtcaga
    32521 tgccagaacg caatttggcg gttattgaag gtttcgtatc tcgtttggta gtgcttgatt
    32581 atgatagtgc tgctgcgacg cataccgggc aaatcagggc cgagcttgcc cggcagggaa
    32641 ggcctgttgg accttttgac cagatgatag cgggacatgc tcgcagtcgc ggtttgattg
    32701 tggttaccaa caatacccga gagtttgagc gagtggcggg aatcagacta gaagactgga
    32761 gctaacattc tcttttactt ctaacattcg taaattatat aacgtggcat gaagccttaa
    32821 ttcctccgcc atttcatccc gctttcctgt ggtaatcttc ctctgaacca cctacgatca
    32881 tttctataat gaatatattg ttgaggtatc tatgcgttgg caagggcgac gtgaaagtga
    32941 caatgttgaa gacaggcgca atagctccgg cggtccgtca atgggcggtc ccggtttccg
    33001 tctgccgagc ggtaaaggcg ggctgatttt actgatagtc gtgctggttg caggctacta
    33061 tggcgttgat ttaaccgggt tgatgaccgg gcagccggtt tctcaacagc aatcaacgcg
    33121 gtcaattagc ccaaatgaag acgaagccgc aaaattcacc tcggtgattc tggcaaccac
    33181 ggaagacacc tggggacaac agttcgagaa gatgggtaag acctatcagc aaccgaagct
    33241 ggtcatgtac cgtggaatga cgcgtaccgg atgcggggcg ggccagtcca taatggggcc
    33301 gttctattgc ccggcggatg gcacggttta tatcgatctc tccttctatg atgacatgaa
    33361 agacaaactt ggcgcggatg gcgattttgc ccaggggtac gttatcgccc atgaagtcgg
    33421 tcatcatgtg cagaaactgt taggcatcga gccgaaagtt cgtcaactgc aacaaaacgc
    33481 gacgcaggcg gaagtgaacc gcttatctgt gcgtatggaa ctccaggccg actgttttgc
    33541 cggtgtctgg gggcatagta tgcagcagca aggcgttctg gaaaccggcg atctggaaga
    33601 ggcgctgaac gcggcgcagg ccatcggcga tgaccgttta caacagcaaa gtcaggggcg
    33661 agtagtacca gacagtttca ctcatggcac ttctcagcaa cgctacagct ggtttaaacg
    33721 tggtttcgac agcggcgatc cggcacaatg caatactttt ggtaaaagca tttaacttcc
    33781 ggggcaggga tggctgaact gactgcgttt cacacattaa cagcgcaaat gaaacgtgaa
    33841 gggatccgcc gcttgctggt gttgagcggg gaagagggtt ggtgttttga tcatgcgctt
    33901 aagttgcgtg atgccttacc tggcgactgg ctgtggattt cgccgcagcc agatgctgaa
    33961 aaccactgtt ctccctcggc actacaaact ttacttgggc gcgagttccg gcatgcggta
    34021 ttcgacgccc gccacggctt tgatgccgct gcctttgcgg cacttagcgg aacgttgaaa
    34081 gcgggaagct ggctggtttt gttactccct gtatgggaag agtgggaaaa ccaacctgat
    34141 accgactcgc tgcgctggag tgattgccct gaccctattg cggcgccgca ttttgtccag
    34201 catttcaaac gtgtacttac ggcggataac gacgctattc tctggcggca aaaccagccg
    34261 ttctcgttgg cgcattttac tccccgtact gactggcaca ccgcgaccgg cgcaccacaa
    34321 ccagaacaac agcaactctt acagcagcta ctgaccatgc cgtcgggcgt ggcagcggta
    34381 acggctgcgc gtgggcgcgg taaatcggcg ctggcagggc aactcatttc tcgtattgcg
    34441 ggtagtgcga ttgtcactgc gcccgcaaaa gcggcaacgg atgtactggc acaatttgcg
    34501 ggcgagaagt ttcgctttat tgcgccggat gccttgttag ccagcgatga gcaagccgac
    34561 tggctggtgg tcgatgaagc cgcagccata cctgcgccgt tgttgcatca actggtatcg
    34621 cgttttcctc gaacgttgtt aaccactacg gtgcagggct acgaaggcac cggacgtggt
    34681 tttttgctga aattttgcgc tcgctttccg catttacacc gttttgaact gcaacagccg
    34741 atccgctggg cacagggatg cccgctggaa aaaatggtta gtgaggcact ggtttttgac
    34801 gatgaaaact tcacccatac accacaaggc aatatcgtca tttccgcatt tgaacaaacg
    34861 ttatggcgaa gcgagccaga aacgccgtta aaggtttatc agttattgtc tggtgcgcac
    34921 taccggactt cgccgctgga tttacgccgg atgatggatg caccagggca atatttttta
    34981 caggcggctg gcgaaaacga gattgccgga gcgctgtggc tggtggatga ggggggatta
    35041 tctcaacaac tcagtcaggc ggtatgggca ggttttcgtc gcccgcgggg taatctggtg
    35101 gcccagtcgc tggcggcgca cggtagcaat ccactggcag cgacattgcg tggacggcgg
    35161 gtcagccgga tagcagttca tccggcgcgt cagcgggaag gcacagggca gcaacttatt
    35221 gctggtgctt tgcaatatac gcgtgacctc gactatcttt cggtgagttt tggttacacc
    35281 ggggagttat ggcgtttctg gcaccgctgc ggttttgtgc tggtgcggat gggtaatcat
    35341 cgtgaagcca gcagcggttg ctatacggcg atggcgctgt taccgatgag taatgcgggt
    35401 aaacaactgg ctgaacgtga gcattaccgt ttacgtcgcg atgcgcaagc tctcgcaaag
    35461 tggaatggcg aaacgcttcc tgttgatcca ctaaacgatg ccgtcctttc tgacgacgac
    35521 tggcttgaac tggccggttt tgctttcgct catcgtccgc tattaacatc gttaggttgc
    35581 ttattgcgtc tgctacaaac cagtgaactg gcattaccgg cgctgcgtgg gcgtttacag
    35641 aaaaacgtca gcgacgcgca gttatgtacc acacttaaac tttcaggccg caagatgtta
    35701 ctggtccgtc agcgggaaga ggccgcgcag gcgctgttcg cacttaatga tgttcgcact
    35761 gagcgtctgc gcgatcgcat aacgcaatgg caattttttc actgactcct tcaattttct
    35821 cccatggtca ttgtgagtca gcggcgtaaa attaatgaca tcagttaagg agttcgccat
    35881 gaaacatgac cattttgttg ttcaaagccc ggataaacct gcgcaacagt tgttactgct
    35941 tttccacggt gtcggggata acccggtggc aatgggggaa ataggtagct ggtttgcacc
    36001 gctgtttcct gatgcgctgg tggtgagtgt cggtggcgcg gagccgagcg gtaatccggc
    36061 ggggcgtcag tggttttcag tgcagggtat tacggaagat aatcgccagg cgcgtgtgga
    36121 tgccatcatg ccgacgttta ttgagacggt gcgctactgg cagaaacaga gcggagtagg
    36181 ggctaacgcc acggcgttaa tcggtttctc tcaaggcgcg attatggcgc tggagagcat
    36241 taaagccgaa ccgggccttg cttcacgcgt cattgctttt aacggacgct atgcgagcct
    36301 gccggaaact gcgtctacag ccacgacaat tcacctgatt catggcggtg aagatccggt
    36361 tatcgatctg gcgcatgcgg ttgcggcgca ggaagcgtta atcagtgccg gtggtgacgt
    36421 gacgctggat atcgtggagg atttaggcca tgcgatcgac aatcgcagta tgcaatttgc
    36481 cctggatcac ttgcgttata ccattccgaa acattatttc gacgaagcgc taagtggcgg
    36541 taagcctggc gatgatgatg tgattgaaat gatgtaataa tagccgggta ggcctgataa
    36601 gactgtatca ggcatcatgg ccggatgtgg cgtgaacgcc ttatccggcc actcagctta
    36661 cttcttcggt tgatcctttt tcggccagtc gtcgtcatcg tcccacttat cgttgaaatc
    36721 acgatgcggg ggaagctccg gcttgttggc gagaaatttc ttatggtcga cgcgcttgag
    36781 atctttaatc acgttcagca ggacgcctac caaaaagaca atcaccagaa tccaccaata
    36841 tttagccagc cagtccatgc ttatttcctc ttgcagaacc actcatcacg cgacgagctg
    36901 ttccatgata cgttgataca tacgggcaag tagctgcagg tcggcagcgt tcacacattc
    36961 attaatttta tgaatagtgg cattgaccgg cccgagttcc accacctgcg cccccatgcg
    37021 ggcaataaag cgcccgtcgg acgttccgcc tgtggtcagt agctgcggtt taatttcatt
    37081 atagtgctca accgcgttaa cgaccgcatc caccagttta ccgcgcgcgg tcaaaaatgg
    37141 ctgcccggaa agccaccagt ccaccgtata acgcagttga tgtttttcaa gcagggcaag
    37201 cacctgcgct ttgatcatct catcagtcag ttcggtgctg aagcggaagt taaactgcac
    37261 aaacagttca cccggaataa cgttgttact gcccgttccc gcctgaatat tagcaatctg
    37321 catactggtc gccgggaaaa attcattgcc ctgatcccac tcaatagcca ctaattcatt
    37381 aaggaaaggt gctgcgcgat gtactggatt gtcagccaga tgcgggtagg ccacatgccc
    37441 ctgaacgcca tgaatggtaa ggttgcaggt taacgatccg cgacgaccat ttttcaccac
    37501 atcaccaacc acttcgatac tcgacggttc gccgaccagg cagtaatcga gacgctcatt
    37561 acgcgacatt aacgcttcga cgacttttac cgtaccgttg tgggcactag cttcttcatc
    37621 agaagtgatc agaaatgcca gtcgccccgt atggttggga tgttgtgcga caaaacgttc
    37681 tgccgctacc accatcgccg ccagcgagcc tttcatatct gccgcaccgc gcccgaataa
    37741 catgccgtca cgaatggttg gttcaaacgg cggattgatc caacgatcgg cgtcgccagg
    37801 cggcaccacg tcggtatgcc cggcaaaggc taacgtttca ccctgcccac gccatgccca
    37861 aaaattctgc gtatcggcaa agtccatgcg ttcaacggta aaaccgatcg cctgcaaacg
    37921 ttcaatcaac aaagcctggc atcctgcgtc atcaggactc agggaagggc ggcgaataag
    37981 ctgttgtgtc agctcaataa ccgggcacga catagactac acctcatgga aaaattgctg
    38041 ataactggaa tcactgaaac ccagcagcat aggcttacca ggcgcgcaga gcaatggacg
    38101 tttgataatt gcaggcattt cagtcattaa tgccgccgca gaggccgcat cggtgatttt
    38161 attgcgggtg gtttcgtcca gtttacgcca ggttgtacca cgggtgttga gtaacgcttc
    38221 ccagcctaat tcgttgataa aacccttcaa taattcgctg tccagcccat cgacgcggta
    38281 atcatgaaaa cgatagtcga tgttattggc ttccagccaa cggcgagcct ttttaatggt
    38341 gtcacaattt ttgatgccgt aaagtgtaac cataatgaat cctttttcga tttatgagaa
    38401 taaatactca tttaagggtg aaaacatttt gcctgcaaat gattatcagg aaa
//
LOCUS       DAPHDW010000030        37958 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.030, whole genome shotgun sequence.
ACCESSION   DAPHDW010000030 DAPHDW010000000
VERSION     DAPHDW010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37958
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.030"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(99..386)
                     /locus_tag="RH057_001509"
     CDS             complement(99..386)
                     /locus_tag="RH057_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HDV3127901.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTVESQKSVEDEDIIRLVAER"
     gene            complement(461..604)
                     /gene="mgrB"
                     /locus_tag="RH057_001510"
     CDS             complement(461..604)
                     /gene="mgrB"
                     /locus_tag="RH057_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HDV3127902.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            763..1002
                     /locus_tag="RH057_001511"
     CDS             763..1002
                     /locus_tag="RH057_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127903.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLGITDCPLLRKSNIVVDNG"
     gene            complement(1145..1936)
                     /gene="kdgR"
                     /locus_tag="RH057_001512"
     CDS             complement(1145..1936)
                     /gene="kdgR"
                     /locus_tag="RH057_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HDV3127904.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLRCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            2113..3486
                     /locus_tag="RH057_001513"
     CDS             2113..3486
                     /locus_tag="RH057_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3127905.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAMRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIATELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMAAGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRSRA"
     gene            complement(3532..4413)
                     /gene="htpX"
                     /locus_tag="RH057_001514"
     CDS             complement(3532..4413)
                     /gene="htpX"
                     /locus_tag="RH057_001514"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HDV3127906.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(4605..6653)
                     /gene="prc"
                     /locus_tag="RH057_001515"
     CDS             complement(4605..6653)
                     /gene="prc"
                     /locus_tag="RH057_001515"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HDV3127907.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNALWDSKVKFDELSLKLAGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FGPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLPKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(6673..7371)
                     /gene="proQ"
                     /locus_tag="RH057_001516"
     CDS             complement(6673..7371)
                     /gene="proQ"
                     /locus_tag="RH057_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HDV3127908.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(7468..7965)
                     /gene="msrC"
                     /locus_tag="RH057_001517"
     CDS             complement(7468..7965)
                     /gene="msrC"
                     /locus_tag="RH057_001517"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HDV3127909.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFGRFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            8095..9378
                     /gene="yebS"
                     /locus_tag="RH057_001518"
     CDS             8095..9378
                     /gene="yebS"
                     /locus_tag="RH057_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HDV3127910.1"
                     /translation="MALNTPQITPTKKITVRSIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDTITGAMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERFY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGRCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSMLDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            9347..11980
                     /gene="yebT"
                     /locus_tag="RH057_001519"
     CDS             9347..11980
                     /gene="yebT"
                     /locus_tag="RH057_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
                     /protein_id="HDV3127911.1"
                     /translation="MSQETPASTTEAQIKNKRRISPFWLLPFIALMIAGWLIWDSYQD
                     RGNTVTIDFMSADGIVPGRTPVRYQGVEVGTVQDISLSDDLRKIEVKVSIKSDMKDAL
                     REETQFWLVTPKASLAGVSGLDALVGGNYIGMMPGKGKEQDHFVALDTQPKYRLDNGD
                     LMIHLQAPDLGSLNSGSLVYFRKIPVGKVYDYAINPNKQGVVIDVLIERRFTDLVKKG
                     SRFWNVSGVDANVSISGAKVKLESLAALVNGAIAFDSPEESKPAEAEDTFGLYEDLAH
                     SQRGVIIKLELPSGAGLTADSTPLMYQGLEVGQLTKLDLNPGGKVTGEMTVDPSVVTL
                     LRENTRIELRNPKLSLSDANLSALLTGKTFELVPGDGEPRKEFVVVPGEKALLHEPDV
                     LTLTLTAPESYGIDAGQPLILHGVQVGQVIDRKLTSKGVTFTVAIEPQHRELVKGDSK
                     FVVNSRVDVKVGLDGVEFLGASASEWINGGIRILPGDKGEMKASYPLYANLEKALENS
                     LSDLPTTTVSLSAETLPDVQAGSVVLYRKFEVGEVITVRPRANAFDIDLHIKPEYRNL
                     LTSNSVFWAEGGAKVQLNGSGLTVQASPLSRALKGAISFDNLSGASASQRKGDKRILY
                     ASETAARAVGGQITLHAFDAGKLAVGMPIRYLGIDIGQIQTLDLITARNEVQAKAVLY
                     PEYVQTFARGGTRFSVVTPQISAAGVEHLDTILQPYINVEPGRGNPRRDFELQEATIT
                     DSRYLDGLSIIVEAPEAGSLGIGTPVLFRGLEVGTVTGMTLGTLSDRVMIAMRISKRY
                     QHLVRNNSVFWLASGYSLDFGLTGGVVKTGTFNQFIRGGIAFATPPGTPLAPKAQEGK
                     HFLLQESEPKEWREWGTALPK"
     gene            12060..13499
                     /gene="rsmF"
                     /locus_tag="RH057_001520"
     CDS             12060..13499
                     /gene="rsmF"
                     /locus_tag="RH057_001520"
                     /EC_number="2.1.1.178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
                     /protein_id="HDV3127912.1"
                     /translation="MAQHTVYFPDAFLTQMREAMPSTLSFDDFLAACQRPLRRSIRVN
                     TLKISVADFLQLTAPYGWTLTPIPWCEEGFWIERDNEDALPLGSTAEHLSGLFYIQEA
                     SSMLPVAALFADGNAAQRVMDVAAAPGSKTTQIAARMNNEGAILANEFSASRVKVLHA
                     NISRCGISNVALTHFDGRVFGVAVPEMFDAILLDAPCSGEGVVRKDPDALKNWSPESN
                     QEIAATQRELIDSAFHALRPGGTLVYSTCTLNREENEAVCMWLKETYPDAVEFLPLGE
                     LFPGANKALTEEGFLHVFPQIYDCEGFFVARLRKTQAIPALPTPTYKVGNFPFSPVKD
                     REAGQIRQAAAGVGLNWDENLRLWQRDKELWLFPVGIEALIGKVRFSRLGIKLAETHN
                     KGYRWQHEAVIALASPDNMNAFELTPQEAEEWYRGRDVYPQAAPVADDVLVTFQHQPI
                     GLAKRIGSRLKNSYPRELVRDGKLFTGNA"
     gene            13617..13853
                     /locus_tag="RH057_001521"
     CDS             13617..13853
                     /locus_tag="RH057_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HDV3127913.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            13958..14149
                     /locus_tag="RH057_001522"
     CDS             13958..14149
                     /locus_tag="RH057_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HDV3127914.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGGCF
                     PIEDFIDGFWRPAQEYGDF"
     gene            complement(14150..14806)
                     /gene="pphA"
                     /locus_tag="RH057_001523"
     CDS             complement(14150..14806)
                     /gene="pphA"
                     /locus_tag="RH057_001523"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
                     /protein_id="HDV3127915.1"
                     /translation="MKQPAPVYQRIAGHQWRHIWLSGDIHGCLEQLRRKLWHCRFDPW
                     RDLLISVGDVIDRGPQSLRCLQLLEQHWVRAVRGNHEQMAMDALASQQMSLWLMNGGD
                     WFIALADNQQKQAKTALEKCQHLPFILEVHSRTGKHVIAHADYPDDVYEWQKDVDLHQ
                     VLWSRSRLGERQKGQGITGADHFWFGHTPLRHRVDIGNLHYIDTGAVFGGELTLVQLQ
                     "
     gene            complement(15202..15543)
                     /locus_tag="RH057_001524"
     CDS             complement(15202..15543)
                     /locus_tag="RH057_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HDV3127916.1"
                     /translation="MMKKSILTFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTKAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(15556..16428)
                     /gene="copD"
                     /locus_tag="RH057_001525"
     CDS             complement(15556..16428)
                     /gene="copD"
                     /locus_tag="RH057_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HDV3127917.1"
                     /translation="MLAFTWIALRFIHFTSLMLVFGFAMYGAWLAPLTIRRLLAKRFL
                     RLQQHAAVWSLISATAMLAVQGGLMGTGWADVFSPNIWQAVLQTQFGGVWLWQIVLAL
                     VTLIVALMQPRNVSRLLFMLTTAQFILLAGVGHATMNEGVTAKIHQTNHAIHLICAAA
                     WFGGLLPVLWCMQLIKGRWRHQAIQALMRFSWCGHFAVIGVLASGVLNALLITGFPPT
                     LTTYWGQLLLLKAILVMIMVVIALANRYVLVPRMRQDEDRAAPWFEWMTKLEWAIGAV
                     VLVIISLLATLEPF"
     gene            complement(16432..16806)
                     /gene="yobA"
                     /locus_tag="RH057_001526"
     CDS             complement(16432..16806)
                     /gene="yobA"
                     /locus_tag="RH057_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HDV3127918.1"
                     /translation="MTSTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYI
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            16945..17175
                     /gene="holE"
                     /locus_tag="RH057_001527"
     CDS             16945..17175
                     /gene="holE"
                     /locus_tag="RH057_001527"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HDV3127919.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            17277..17933
                     /locus_tag="RH057_001528"
     CDS             17277..17933
                     /locus_tag="RH057_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HDV3127920.1"
                     /translation="MSFWKVAAAQYEPRKTSLTEQVAHHLEFVRAAARQQCQLLVFPS
                     LSLLGCDYSRRALPAPPDLSLLDPLCYAATTWRMTIIAGLPVEYNDRFIRGIAVFAPW
                     RKTPGIYHQSHGACLGRRSRTITVVDEQPEGMDMDPTCSLFTTGQCLGEPDLLASARR
                     LQFFSHQYSIAVLMANARGNSALWDEYGRLIVRADRGSLLLVGQRSSQGWQGDIIPLR
                     "
     gene            17957..18619
                     /gene="exoX"
                     /locus_tag="RH057_001529"
     CDS             17957..18619
                     /gene="exoX"
                     /locus_tag="RH057_001529"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HDV3127921.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSHLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGIKYSNMALYKTRKLNVQTPPGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERDPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(18616..20676)
                     /gene="ptrB"
                     /locus_tag="RH057_001530"
     CDS             complement(18616..20676)
                     /gene="ptrB"
                     /locus_tag="RH057_001530"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HDV3127922.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPEVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWEILLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDKLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(20885..21544)
                     /locus_tag="RH057_001531"
     CDS             complement(20885..21544)
                     /locus_tag="RH057_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HDV3127923.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVPGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDAKERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVRDGIERDLEQQKRTLA
                     E"
     gene            complement(21871..22227)
                     /locus_tag="RH057_001532"
     CDS             complement(21871..22227)
                     /locus_tag="RH057_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HDV3127924.1"
                     /translation="MKKRGAFLGLLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVSVDCKAGMAEYQRR"
     gene            complement(22294..22584)
                     /gene="yebG"
                     /locus_tag="RH057_001533"
     CDS             complement(22294..22584)
                     /gene="yebG"
                     /locus_tag="RH057_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
                     /protein_id="HDV3127925.1"
                     /translation="MAVEVKYVVIREGEEKMSFTSKKEADAYDKMLDTADLLDTWLTN
                     SPVQMEDEQREALSLWLAEQKDVLSTILKTGKLPSPQVVGAESEEEDASHAA"
     gene            22718..23896
                     /gene="purT"
                     /locus_tag="RH057_001534"
     CDS             22718..23896
                     /gene="purT"
                     /locus_tag="RH057_001534"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HDV3127926.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     SDAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSESLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRRLGVALATAESVVDAIERAKHAAGQVKVQG
                     "
     gene            complement(23952..24593)
                     /gene="kdgA"
                     /locus_tag="RH057_001535"
                     /gene_synonym="eda"
     CDS             complement(23952..24593)
                     /gene="kdgA"
                     /locus_tag="RH057_001535"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HDV3127927.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(24630..26441)
                     /gene="edd"
                     /locus_tag="RH057_001536"
     CDS             complement(24630..26441)
                     /gene="edd"
                     /locus_tag="RH057_001536"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HDV3127928.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPEDKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            complement(26676..28151)
                     /gene="zwf"
                     /locus_tag="RH057_001537"
     CDS             complement(26676..28151)
                     /gene="zwf"
                     /locus_tag="RH057_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HDV3127929.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKAGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFSRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            28489..29358
                     /gene="hexR"
                     /locus_tag="RH057_001538"
                     /gene_synonym="yebK"
     CDS             28489..29358
                     /gene="hexR"
                     /locus_tag="RH057_001538"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HDV3127930.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAAMALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            29486..30928
                     /gene="pyk"
                     /locus_tag="RH057_001539"
     CDS             29486..30928
                     /gene="pyk"
                     /locus_tag="RH057_001539"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HDV3127931.1"
                     /translation="MSRRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPADVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVHFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(31059..32030)
                     /gene="lpxM"
                     /locus_tag="RH057_001540"
                     /gene_synonym="msbB"
     CDS             complement(31059..32030)
                     /gene="lpxM"
                     /locus_tag="RH057_001540"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416369.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HDV3127932.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVDWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IARRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(32150..33472)
                     /gene="mepM"
                     /locus_tag="RH057_001541"
     CDS             complement(32150..33472)
                     /gene="mepM"
                     /locus_tag="RH057_001541"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HDV3127933.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            complement(33488..34420)
                     /gene="znuA"
                     /locus_tag="RH057_001542"
     CDS             complement(33488..34420)
                     /gene="znuA"
                     /locus_tag="RH057_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HDV3127934.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            34499..35254
                     /gene="znuC"
                     /locus_tag="RH057_001543"
     CDS             34499..35254
                     /gene="znuC"
                     /locus_tag="RH057_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HDV3127935.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNRPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            35251..36036
                     /gene="znuB"
                     /locus_tag="RH057_001544"
     CDS             35251..36036
                     /gene="znuB"
                     /locus_tag="RH057_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HDV3127936.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLIAIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQRVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFILSMMKKQAS"
     gene            complement(36183..37193)
                     /gene="ruvB"
                     /locus_tag="RH057_001545"
     CDS             complement(36183..37193)
                     /gene="ruvB"
                     /locus_tag="RH057_001545"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HDV3127937.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGALEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(37202..37813)
                     /gene="ruvA"
                     /locus_tag="RH057_001546"
     CDS             complement(37202..37813)
                     /gene="ruvA"
                     /locus_tag="RH057_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HDV3127938.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDASSETLIREALRAAL"
BASE COUNT         9098 a         9789 c         9354 g         9717 t
ORIGIN      
        1 agagttttaa tttgaagcag ttgcaccatg gcagtgcgta taatcaggat gaattgagta
       61 acccttcaac gacggggtta ctcaagattg aggaaggatt atcgttcggc gaccagacga
      121 ataatatctt cgtcttcaac cgatttttga ctctcaacgg tctttttatc tgctttctca
      181 ggttcgtttg cttcgcacaa acgacgtaac agtgcatttt gacgtttttg ctgatccagc
      241 aacgcctcaa gcagttcaat ctgttcgtta gtccgagaac tggcacgatt gataaaaaac
      301 cacaagatga gcccgataag aagaaccacc accgatacaa ccaaagacgc aatgttcatc
      361 acgcctgaat ttacaacttc gttcatttca ccacctctga gtagaggcgc tattctacca
      421 ctgctggaga ggaagaaaat ctagtgctga aaaaatgata tcaccacggg ataaactggt
      481 taatggcaca aattccgctg aaaaattgta catcctgatc gcacatcatg ttgaatacct
      541 gcgcccaaag cagcaagcaa gccaacacca cgacaaccag aacgacccat cgaaactttt
      601 tcactccaat ctccgcttca cctaccttat gtcattaaac tagcatgcgt tagttaaaca
      661 gcgcctaact atgtcgatat tcgtgctttt tcggaatgag tagcttgctt cagcgcatat
      721 tgccgctacg attaagcgaa cataaaaaga gaagaggttg taatgcgatt catcattcgc
      781 acagttatgc tgatagcact ggtatggata ggattattac tcagcggata tggcgttttg
      841 attggaagca aagagaatgc cgcaggatta ggtttgcaat gtacctatct gactgccaga
      901 ggcaccagca cggtacaata cttgcacact aaaagtggtt tcttggggat aacagattgc
      961 ccactgttac gtaaaagcaa tatcgtggtc gataatggtt aagggtaata aataaccaga
     1021 ttctcactca tgagtaaaat attcttatag caagcgcgtt taatataacg caccttcaac
     1081 tttatttttc tcatgctaaa taaagaacct gtctgtttga tattccgaca ggttcttact
     1141 ctcatcagaa cggatagtcg tgataaccca tttgggcaga aattttgcgc gctgcggtat
     1201 gcaacattgc gacatattcc tgtaaacgct cttcagagaa acgcaacgtc gggaaggaga
     1261 tgctcaaacc tgcaatgacc acgccaaagc gatcaaatac cggtaccgca atgcatcgca
     1321 gcccttcttc ctgctcttcg ttatcttcgc catacccctg ctcgcgcacc tggtccagaa
     1381 cgggtaataa cgcttctgta ctggtgatgg tccgctcggt actgcgttta tactccacgc
     1441 cctcaagaat ttgcttcact tcatcgcgat cgcgccatgc cagcagtacc ttaccaatcg
     1501 cggtgctgta cagcggatta cgacgcccaa tccgtgaata catgcgcaaa ttgtacatag
     1561 agtcaatttt gtgaatgtaa acaatactgt cttcgtccag tgcgccgagg tggatagttt
     1621 ctttggtcag gcgggagatc tcacgcatct ggatatctgc gctacgaatt aaatcgacgt
     1681 tttgtaacgc gcgagcgccc agttcaaaca atttcagggt cagggaatat ttctccgact
     1741 ccccttcctg cgccacataa cctaaggttt tcatggtctg taaaaagcga taaacggtgc
     1801 tttttgacat catgacgcgc tgcgacagct cggttatccc tatttcgcgc tcttcaccca
     1861 gcgcctgcaa aatgccaaaa acttttagca cggaagatac agaatcaggc tgtttatcca
     1921 gatctgcgtt agccatttat cacctcattg cgagtgtttt ataaaaatca gaactgtttt
     1981 ttattataat ttcgcaccag ggtggtcgca atccatcttt tgccggttag ttacaattct
     2041 gcgacatcca ccgtgaatat cagtgctaga atcatacccc tgttgattat tcaccaaaga
     2101 tataaaattc ctatgccaaa agttcaggcc gacggcctgc cattgcccca gcgatacggt
     2161 gcgatattaa ccattgtgat tggtatttcg atggctgtcc ttgacggcgc aatcgccaac
     2221 gtcgccctgc caacaatcgc cacggacctt catgccacgc cagccagttc catctgggta
     2281 gtgaacgcct atcaaatcgc cattgtcatc tccctgctct cgttttcgtt tctgggcgat
     2341 atgtttggct atcgacgtat ttataaatgc ggtctggtcg tttttctgtt gtcttcactg
     2401 ttctgcgccc tttctgattc gctgcaaatg ctcacccttg cgcgtgtcat acaaggtttc
     2461 ggcggtgcag cgttgatgag cgttaatacc gcacttatcc gcctgatcta tccacaacgt
     2521 tttctgggga gagggatggg cataaactcg tttattgttg ccgtttcttc tgctgccggg
     2581 ccgacaattg ctgcagcaat cctctccatc gcatcctgga aatggttatt tttaatcaac
     2641 gtaccgttag gtattatcgc cctgcttctg gcgatgcgtt ttctgccacc caatggttct
     2701 cgcgccagta aaccccgttt tgacctgccc agcgccgtga tgaacgcgtt aaccttcggc
     2761 ctgcttatca ctgcgttgag tggtttcgct caggggcaat cgctgacgtt aattgctacg
     2821 gaactggtgg taatggttgt tgttggtatt ttctttattc gccgccagct ttctcttccc
     2881 gtaccgctgc taccggtgga tttactgcgt atcccgctgt tttcactttc tatttgcaca
     2941 tctgtttgct ctttctgcgc acaaatgctg gcaatggttt ccctgccctt ttacctgcaa
     3001 accgtgctcg ggcgtagtga agtcgaaaca ggtttacttc tgacaccgtg gccgttagca
     3061 acgatggtga tggctccgtt ggcaggctat ttgattgaac gcgtacatgc aggattgctg
     3121 ggtgctctag ggttgttcat catggctgcg gggctttttt ccctggttct gctgcccgcg
     3181 tcacctgcgg atatcaatat tatctggccg atgatcttat gtggcgctgg atttggcttg
     3241 ttccagtcac ccaataacca caccattatt acctccgctc ctcgcgaacg tagcggtgga
     3301 gccagtggca tgttaggaac ggctcgtcta ctgggtcaga gtagcggcgc ggcactggtg
     3361 gcgctgatgc taaatcagtt cggtgataat ggtacgcacg tttcgctgat ggctgcggct
     3421 attctggcag tgattgctgc ctgtgtcagt ggtttacgta tcactcagcc acgatccagg
     3481 gcataaaaaa agcgcgtcga tcaggacgcg ctttttaata tttacttcat attacttcag
     3541 gtattcaccc gtacgcagag cttcaattcg tttatccagc ggcgggtgag tcatgaacaa
     3601 ctcactgagc gatttcgact taccgttaat gcagaaagcc atcatgctgg ttgcttcttg
     3661 cggttcatag ctggttttca ggcgctgcag cgcggcaatc attttctcgc gaccaaccag
     3721 ttttgccgaa ccggcatcag catggaattc acgatgacgc gagaaccaca tggtgataat
     3781 gctcgccaga ataccaaaca ccagttccag aaccgttgca accgcaaagt agatcagcgg
     3841 gttgccgttg ctctcttcac cttcatcacg atttccgccc ataaaacccg cggcaagctg
     3901 cgccagaata cgggaaataa agataacaaa ggtgttcacc acgccctgaa tcagcgtcat
     3961 ggtgaccata tcaccattgg cgatgtggct gatttcgtga gcaattaccg cctcggcttc
     4021 atccgggctc atgttctgca gcaaaccggt gctgacagca accagagagg catcacggcg
     4081 cgcaccggtt gcaaaagcgt tgatgtccgg cgcatggtag atagccactt gcggcatagc
     4141 gatccctgcc tgacgagcct gggttgctac agtattaacc agccaacgtt ccctttcgtt
     4201 acgcggttgc tcgatcactt ccccgccaac agatcgtaat gccatccatt tggacatcag
     4261 aagcgaaacg aaggaaccac caaaaccgaa cagcaaggcc atgatcatca gcccctgaac
     4321 gctgctcgac tgtatccctg tcaggctcag taccagcccg aaaacgacca ttacggccag
     4381 gttcgttagc aggaagagcg cgattcgcat cataattttc tttttacctc agtttaacaa
     4441 aacgcaatat gcgataccca catcgtatgg gttacgcgac tattttcaag tctggatagt
     4501 gcgtaagtca ccagaaagac acaactttac attttgtagc atctgattta cggcatcttg
     4561 ccgctgttaa aaaatcaggc acaatttctt gtgcctgatt gatattactt gacgggagcg
     4621 ggttgttccg cgggtctggc tttttcaagc ttcgccagat cgagtgcgat attcaccgtc
     4681 tcatccagat aaggatccgg ctcctggtaa tctttaggta gatcatccag cttcttcaac
     4741 tccggtttac cttcgcgttt aaagcgttcg ttcaaacgcg ccagacgcgt cgcatcatct
     4801 tcattattct ctttctcacg cacagcgtag ttcagagaaa cgatattgcg cttgtccttc
     4861 atagcgttga agcgcgcgat atccttcatg atgttctgga actcaggatc tttcgcgata
     4921 cgcgcattat gttccttcag cagctccggc ccaaaggccg ttaaatcgcc tgatttaaca
     4981 taagtcgcgg catcaatgct atcccacggc agcgcgttat cttcgaattt ctcacccgtt
     5041 tccgtttctt cattacccgt cggcatgatg atgtctggcg ttacgccttt acgttgcgta
     5101 ctgccgccgt taacgcgata gaatttctgg atcgtgtact gcacagaacc cagcgctggc
     5161 cattcaggac gtaacatctg atcgtaaata cggttcaatg aacggtattg ctgaacggtg
     5221 cctttaccaa acgtcggttc acccacaacc agcgcacgac cgtaatcctg cattgccgcg
     5281 gcaaagattt ctgaagccga agcactgaaa cggtcaacca gtaccaccag cgggccttta
     5341 tagaacacct gcccgtcggt atcgctgtct tcacgaacct tgccgttgtt atcgcggacc
     5401 tgaacaatgg gacccgcagg aataaacaga cctgagagcg ataccgcttc ggtcaacgcc
     5461 ccaccgccat tgctacgcag gtcgatgatc acactgctga cattctgttt ttccagtttc
     5521 tgcagttgaa ctttgacatc gtctgtcaaa cccacataga agcccggaat atccagcacg
     5581 ccgactttct ctttaccgac ggtcttcacc gacattttaa ccgcgcggtc ttcgagacga
     5641 atacgttcac gggtcaatgt tacagtgcgg gtcttggtcc ctttaccagc aggtaaaatt
     5701 tccagacgaa ctttactgcc cttcggccct ttaattaagg caaccacatc atcaagacgc
     5761 cagccaatca cgtcaaccat cggcttgcct gtttgaccaa caccgacaat tttgtcacca
     5821 acgctgatag ctttactctt cgctgccgga ccacctgcca ccatcgaatt gataacggtg
     5881 tagtcatcat ccatttgcag cactgcgcca ataccttcca gcgacaaact catttcagtg
     5941 ttgaactgtt cggtattacg cggggaaaga tagttggtat gcgggtcgat ttcacgcgca
     6001 aacgccgtca ttgccagcga gaaaacatct tcgctgttgg tttgcgccag acgacgaatg
     6061 gcaaatttgt agcggcgtgt cagggtttca cgaatttctt tatccgtttt tcctgccagc
     6121 ttcaggctta actcgtcgaa tttgacttta ctgtcccaca gcgcgttcaa ctcagcctcg
     6181 tttttcggcc agggcgcttt gctgcggtca aggttataag tgtcgttgcc ggtgaaatcc
     6241 atcggctttt ccagtaccga caaagcgtac tggtaacgct caaaacggcg cttttgcgcc
     6301 agattgtaga gatcgtagaa aacgtcgagt ttgcctgaac gcagttcatc gcctaactcg
     6361 gttttctttt tcgcgaactg ttcaacatcg cttgccagca gcacgttgtg gctgtaatcg
     6421 agcagattca ggtagcggtc aaagattttg gccgaaaatg cctgatcgag gtcgaactgg
     6481 cgataatgag aacgggtgaa gcgcgacgtt acgcgctcac ttaccgtcgc atgctgcgtc
     6541 tcttccttta ataccggaat ttgatcagca cgcgtgatat cttctacagc gaaggtctgg
     6601 cctgctattg caagcaggcc agctaacgcg gtaagcctaa aaaacatgtt catgcctggc
     6661 ccggcctccg tttcagaaca ccaggtgttc tgcgcgcaca atcaaagaca tacccgaatt
     6721 cagctggacg cggacgccgt ctttggtgat ttctaatacg gtggcatcca tcgcgttttg
     6781 acccgctttc accttcagag cttgtccgac agtcagagct gaaatgtcag aaaccggggt
     6841 gtgctgttct tcgcgaggtg cttttactgt ttttggcgct ttctctaccg gcttttgcgc
     6901 acgaggttta cgttcagcgc cttctttgcg gcgtggcgtg gtcggacgtg gcttgcgttc
     6961 gcggcgcggt gcgtcttctt tctcaccagc agctgcggca gcttcgcgtt ttttcgcttg
     7021 ctgttcagca cgctgtgcct gaacacgcgc tttcgcttct tcaagctgct tgcgagcatg
     7081 ctctacatgt tgctcgtcca gctcaccgca tgggttgccg tcaagatcga cacgcgttgc
     7141 gccgggttta acaccgtaaa gataacgcca gctcgaagtg tagagacgta aagcggatcg
     7201 taattgcgtt ttgctcaggt tcatttcccc ggcaacacga tcgaccaaat cctgaaaaat
     7261 accgattttc agcggacgcg cttcaccttc cgcactgaaa cagtggggaa aacgttcggc
     7321 cagaaacgcg attacttctt tactgctatt caacttaggt tgattttcca tgaaatttcc
     7381 tgattacaac ggacgtagcc aacaagcgca ggcatgaaca ggcgtcatta taatgacgct
     7441 accagtaaat gctacgttat ccgttgatta tcctgcgacg ctcgcaaaga attttttgta
     7501 atccgtcgtt gcaagcactt tttcaagctg tgccacaagc tgacgtaagc cttgctcgtc
     7561 ctcgtctgta aagcgaccga agacggtact atcgatgtca agaacaccaa taatctgatt
     7621 tttcaccacc agcggcagaa caatttcaga attactcgcc gcatcacagg caatatgccc
     7681 gtcaaacgcg tgaacatcct cgatacgctg cacttgattg cgggcaaccg cagtgccgca
     7741 caccccgcgc ccgacgggta tccggacaca ggcaattttg ccctgaaatg gtcctagtac
     7801 cagtgtatcg tcctcaagca aataaaaacc tgcccagttt acgtcagtga gacgctcgta
     7861 taacaacgca ctggtgttcg caagcgttgc cagaaaactg gtttctcctg ccatcagcgc
     7921 gttaaagtcg cgatttaaat ccgcgtaaaa ttctgttttg ttcattatat aatcacttgg
     7981 ttgtcttacc tggaactgcc agcctattaa aataagcatt aaatgcgtta atgctcaaga
     8041 tcattcccat catgggttaa tattaatgtt aattcttatt acatttggca cgtcatggct
     8101 cttaacacac cacaaattac gccgacaaaa aagataacag tgaggtcaat cggcgaggaa
     8161 ctgccgcgtg gtgattacca acgttgcccg caatgcgaca tgctgtttag cctgcccgag
     8221 ataaattctc atcagagtgc ctattgtccg cgctgtcagg caaaaattcg cgacgggcgc
     8281 gactggtcgc taacgcgcct ggcggcaatg gctttcacca tgctgttgct gatgccgttt
     8341 gcctggggcg aaccgctgtt gcatatctgg ctgttaggta ttcgtatcga cgccaacgtc
     8401 atgcaaggca tctggcaaat gaccaaacag ggcgatacga taacgggggc gatggtcttt
     8461 ttctgcgtta ttggtgcccc cctcattctg gtgtcctcca tagcatattt atggtttggt
     8521 aaccgactgg gaatgaattt acgtccggta ctgctgatgc ttgagcgact taaagagtgg
     8581 gtaatgctgg atatctacct ggtcggcatt ggcgttgctt ctataaaggt acaggattat
     8641 gcccatatcc aggcgggtgt cggcttgttc tcttttgtgg cgttggtgat tttaacgacg
     8701 gtgacgttgt cacatcttaa tgtcgaggag ctgtgggagc gattttatcc gcagcgcccc
     8761 gctacgcgta gggacgagaa acttcgtgtc tgtcttgggt gccattttac cggctatcct
     8821 gatcagcgtg gtcgctgccc gcgttgccat atcccgctac gcctgcgtcg ccgtcatagt
     8881 ttgcaaaaat gctgggcggc gctgttagcg tcaatcgttt tgttgttacc tgccaacctg
     8941 ttgcctattt ctatcattta tctgaacgga ggacggcagg aagatacaat tctttccgga
     9001 attatgtcgc tggcaagtag caacattgcg gttgcgggaa tcgtgtttat cgccagtatt
     9061 ctggtaccgt ttactaaagt gatcgtcatg ttcactttac tgttgagcat tcattttaaa
     9121 tgccagcaag gtttacgcac acgcattctg ttactgcgga tggtgacctg gattggtcgc
     9181 tggtcgatgc tcgacctgtt tgtcatatct ttaaccatgt cgctgattaa tcgcgatcag
     9241 atcctcgctt ttactatggg accggctgcg ttttatttcg gcgcagcggt aattttgact
     9301 attcttgctg tggaatggct ggacagccgc ttactttggg atgcacatga gtcaggaaac
     9361 gcccgcttcg acgactgaag cgcagattaa aaataaacgc cgtatctcac ctttctggct
     9421 gctgccgttc atcgcgctaa tgattgccgg ttggctgatt tgggacagtt atcaggaccg
     9481 gggtaatacc gtcaccatcg actttatgtc ggcggatggt attgtcccgg gccgtacgcc
     9541 tgttcgttat cagggcgttg aagtcggaac agtgcaggat atcagcctca gcgacgatct
     9601 tcgtaagatt gaagtcaagg tcagcatcaa gtccgatatg aaagatgcgc tgcgcgaaga
     9661 gactcagttc tggctggtga cgccaaaagc atcgttggca ggtgtctccg ggctggacgc
     9721 cctcgtcggt ggtaactata tcggcatgat gccaggtaaa ggtaaagagc aggatcactt
     9781 tgtcgcactc gatacccaac cgaaatatcg actggacaat ggcgatctga tgatccacct
     9841 gcaagccccc gatctcggtt cgctgaacag cggttcattg gtctatttcc gcaagatccc
     9901 tgtgggaaaa gtctacgact atgccatcaa tcccaacaag caaggcgtgg tgattgatgt
     9961 cctgatcgag cggcgtttta ccgatctggt gaaaaaaggt agccgtttct ggaacgtttc
    10021 cggcgttgat gccaatgtca gtatcagtgg cgcgaaggtg aaactggaaa gtctggcggc
    10081 actggttaac ggtgcgattg ccttcgattc acctgaagag tcgaaacctg ccgaggcgga
    10141 agataccttt ggtctgtatg aagatctggc ccacagccag cgtggcgtaa taataaaact
    10201 ggaactgccg agtggggcag gattaaccgc cgactcgacg ccattaatgt atcagggact
    10261 ggaagtcgga cagctaacta aactggattt aaatcctggt ggtaaagtca ctggggaaat
    10321 gacagttgat cccagcgtcg ttaccctgct tcgtgaaaat acccgcatcg aattacgtaa
    10381 tccgaaatta tcccttagcg atgccaatct cagcgccctg ctgactggca aaaccttcga
    10441 gttggtaccc ggcgatggcg agccacgcaa agagttcgtt gttgtgccag gcgaaaaagc
    10501 actgctgcat gaacctgatg ttctgacgct gaccctgacc gcgccggaaa gttacggtat
    10561 tgatgctggt cagccgctca ttcttcacgg cgtgcaggta ggccaggtta tcgatcgtaa
    10621 actcaccagc aaaggcgtca cctttaccgt cgccatcgag cctcagcatc gagaactggt
    10681 aaaaggcgat agcaaatttg tcgtcaacag ccgtgtcgac gtgaaggtgg ggctggatgg
    10741 cgttgagttt ctcggtgcca gcgcctcaga atggattaac ggcgggatac gtattctgcc
    10801 gggcgataaa ggcgagatga aagccagcta tccactgtat gccaatctgg aaaaagcgct
    10861 ggagaacagc cttagcgatt tacccaccac aaccgtgagt ttgagtgcag agacgctgcc
    10921 ggatgtgcag gcaggatcgg tagtgctcta ccgtaaattt gaagttggtg aagttattac
    10981 cgtccgtccg cgagctaacg cgtttgatat cgatctgcat attaagccgg agtatcgcaa
    11041 ccttctgacc agcaatagcg tgttctgggc agaaggcggg gcgaaagttc agctgaatgg
    11101 tagtggcctg accgtacagg catccccgct ctccagagca ttaaagggag ccattagctt
    11161 cgataacctc agcggtgcca gcgccagtca gcgtaaaggc gacaaacgaa ttctgtatgc
    11221 ttccgaaaca gcggcccgtg cggttggtgg gcagattacg cttcacgctt tcgatgccgg
    11281 aaaactggcg gtcgggatgc caattcgcta tctcggtatt gatatcgggc aaatccagac
    11341 gctggatctg attaccgcgc gcaatgaagt acaggcaaag gcggtgctct atccggaata
    11401 tgtccagacc tttgctcgcg gtggtacgcg cttctcagtg gtcacaccgc aaatttcggc
    11461 agctggcgtt gagcatcttg atactatcct ccagccgtat atcaacgtcg aaccaggccg
    11521 gggcaatcct cgccgcgact ttgaattaca agaggccacc attactgatt cgcgttacct
    11581 ggatggctta agcattattg ttgaagcgcc ggaagccggt tcgttaggca tcggtacgcc
    11641 tgtgctgttc cgtggtctgg aagtcggtac ggttacagga atgacgctgg ggacattgtc
    11701 agatcgcgtg atgattgcga tgcgcatcag taaacgctat caacacctgg tgcgtaacaa
    11761 ttccgtcttc tggttggcat cgggttacag tctggacttt ggtctgacgg gcggcgtagt
    11821 gaaaaccggc acctttaacc agtttatccg tggcggcatc gccttcgcca cgcctccggg
    11881 tacgccactg gcaccgaaag cccaggaagg caaacacttc ctgttgcagg aaagtgaacc
    11941 gaaagagtgg cgtgaatggg gaactgcgct tcccaaataa tgcccactgc tccggcgtgc
    12001 ctgcgccgga gcgtttatgc taaactgcgc gcctgttttt ttgccagtgg tacatgctcg
    12061 tggcccaaca caccgtttat ttcccggacg cctttctgac gcaaatgcgc gaagcgatgc
    12121 cttcgacgct ctcttttgat gattttcttg ccgcctgtca gcgcccgttg cgccgcagca
    12181 ttcgcgttaa tacgctgaaa atctccgttg ctgatttcct gcaattaacc gctccttatg
    12241 gctggacgct tacgccaatt ccgtggtgtg aagaaggttt ctggattgaa cgcgacaatg
    12301 aagatgcatt gccattgggt agtaccgccg agcatttaag cggcctgttt tatattcagg
    12361 aagccagttc aatgttgccc gtcgccgcct tgtttgctga cggtaatgca gcacagcggg
    12421 tgatggatgt cgctgctgcg ccaggctcca aaacgacgca aattgccgcg cggatgaata
    12481 acgaaggggc aatccttgcc aatgagtttt ccgccagtcg ggtaaaagtg ttacatgcca
    12541 atatcagccg ctgtggcatc agtaatgttg cgctcacaca ttttgatggc cgcgtgtttg
    12601 gtgtggcagt gccagaaatg ttcgatgcca ttttgctgga cgctccctgc tctggcgaag
    12661 gcgtggtgcg taaagatccc gatgcgctaa aaaactggtc accagaaagc aatcaggaaa
    12721 tcgcagctac acaacgggag cttatcgaca gcgcctttca tgcattacgt cctggtggta
    12781 cgctggttta ctcgacctgt accttaaaca gggaagaaaa cgaagccgtt tgcatgtggc
    12841 tgaaagagac ttaccctgac gcagtagagt ttttaccact tggcgagctc ttccctggtg
    12901 caaacaaagc gctgaccgaa gaaggctttt tgcatgtttt cccacaaatt tacgactgcg
    12961 aaggcttctt cgttgctcgt ctgcgtaaaa ctcaggccat tcccgcctta cccaccccaa
    13021 catacaaagt gggcaatttt ccgttcagcc cggtgaaaga tcgcgaagct ggacaaattc
    13081 ggcaggcggc tgcaggtgtt ggcttaaact gggatgaaaa cctgcgcctc tggcagcgtg
    13141 acaaagaact gtggttgttc ccggtgggca ttgaagccct gatcggtaaa gtccgatttt
    13201 ctcggttggg gattaaactt gccgagacgc acaacaaagg ttatcgctgg cagcatgaag
    13261 cggttattgc tcttgcctca cccgataata tgaatgcatt cgagctgaca ccgcaggaag
    13321 cggaggagtg gtatcgcggg cgcgatgttt acccgcaagc cgcgccagtg gcggatgatg
    13381 tattggttac tttccagcat caaccgattg gtttagccaa acggattggt tcgcgattga
    13441 aaaacagcta tccgcgtgaa ctggtgcgcg atgggaaact ttttaccggt aacgcctgac
    13501 agcgcacaaa aaaagcgcac tttttgactg gcacattcgg cggcctcaac taggctgaaa
    13561 aatggtgcga tcggactggt cgtaccacaa tcggcagcta aatggagagc acgaagatga
    13621 aaaccagtgt gcgcataggc gcttttgaaa tcgacgacgg cgaattacac ggtgaatcgc
    13681 cgggtgatcg aacgttaacc attccttgta aatctgaccc cgatttatgt atgcaactcg
    13741 atgcctggga tgctgaaacc agtatccctg ccctgctaaa tggcgaacac tctgtccttt
    13801 accgtacccg ttacgatcaa caatctgatg cctggattat gcgtcttgcc tgatccaaaa
    13861 agaacccgtc ggcatggcgg gttatttgtc ctggttattc ccccgttgta aaatctctcc
    13921 taaacttaac ggtacggcac cacacttcgg ggatgaaatg ttcgcgctgg tactttttgt
    13981 ttgctacctg gatggcggtt gtgaagatat tgttgtggat gtctacaaca cggaacagca
    14041 gtgtctttat tctatgagcg atcaacggat ccgccatggc ggttgttttc cgattgagga
    14101 ttttatagat ggtttctggc gacctgcaca ggagtacggt gatttttaat tattgcaatt
    14161 gcacaagagt cagttcgccc ccaaagacag caccggtatc aatataatgc aggttgccaa
    14221 tatccacgcg atgtcgcaac ggtgtatgac caaaccagaa atgatcagca cctgtaattc
    14281 cctgcccttt ttggcgttca cctaatcgcg agcggctcca caagacctga tgcaaatcaa
    14341 cgtccttttg ccattcataa acatcatctg gataatcggc atgagcaata acatgtttgc
    14401 cggtacgact gtgtacttca agaataaagg gcaaatgctg acatttttcc agcgccgttt
    14461 tcgcttgttt ctgttgatta tctgccagcg caataaacca gtcgccgcca ttcatcaacc
    14521 acaaagacat ctgctgagat gccagcgcat ccatcgccat ctgttcatga ttgcctctta
    14581 ccgcacgaac ccaatgttgt tccagtaact gcagacaacg taaactttgc ggcccacgat
    14641 cgataacgtc tcctactgag ataagtaaat ctcgccacgg atcaaaacga caatgccata
    14701 atttgcggcg caactgctca agacaaccgt gtatatcgcc agaaagccag atatgtcgcc
    14761 attgatgacc cgcaattctc tgataaacgg gcgcaggctg tttcatcaat attttcctcc
    14821 cgcgctaaag atcacataat cttaacaaga atgttaaaaa acgctggact cagacagtag
    14881 agtgtgtgtt atggttgact ataaagtcag cgaaggaaat gcttctggct tttaacagat
    14941 aaaaagagac cgaacacgat tcctgtattc ggtccaggga aatggctctt gggagagagc
    15001 cgtgcgctaa aagttggcat taatgcaggc ttagttgcct tgccctttaa gaatagatga
    15061 cgacgccagg ttttccagtt tgcgtgcaaa atggtcaata aaaagcgtgg tggtcatcag
    15121 ctgaaatgtt aaaaaccgcc cgttctggtg aaagaactga ggcggttttt ttattggaaa
    15181 tcaaaaggct attttaggta attaacagag tttttcagct cgttctataa acggtgccag
    15241 actcattttt tcgccgggat tgttaggatc atcaatctga atcaccgaaa tgggttgggc
    15301 tttagtcttc ccactggcaa cttccttttg tgcgatatcg tttaaaggat actgcacgag
    15361 ggtacttggg ttaatgacat acaaagcatt acccggtctg caagtcagca tcacctcttc
    15421 gcgattaaac gcccatttgt ctttacccac ttcaaaacga ctgacggtaa tcacctgcgg
    15481 tgcagccagc gccgctgcag aactggtgag taacagaaac gtcagaatac tttttttcat
    15541 cattttcgta atccatcaaa aaggttcgag agtcgcaagc aggctgatga tcaccagcac
    15601 taccgcacct atcgcccatt cgagtttagt catccactca aaccagggtg ccgcacgatc
    15661 ttcatcctgc ctcatgcgcg gtacaagaac ataacgatta gccagcgcaa tgaccaccat
    15721 aatcatcacc aggatggctt tgagcaataa gagttggccc cagtaagtcg taagcgtggg
    15781 gggaaatccg gtaatcaaca atgcattaag cacgccgctt gccagtacgc cgatcaccgc
    15841 aaaatgcccg caccaggaaa aacgcatcag cgcctgaatg gcctggtgtc gccaacggcc
    15901 tttgatgagt tgcatacacc agagcaccgg cagtaatccc ccaaaccacg ccgccgcaca
    15961 aatcaggtga atcgcgtgat tagtctgatg gattttcgcc gttacccctt cattcatcgt
    16021 cgcatgcccc actcccgcca gcagaataaa ttgcgcggta gtgagcataa acagcaaacg
    16081 tgacacattc cgcggttgca taagggcgac gatcaacgtt acgagggcga gaacaatttg
    16141 ccataaccag acgccaccaa actgcgtttg caaaacagcc tgccaaatgt ttggcgaaaa
    16201 tacatctgcc catcccgttc ccatcaaccc gccctgaacg gcgagcattg cagtggcact
    16261 gatcaaactc cagacggcag cgtgctgttg taaacgcaga aaacgcttcg ccagcaagcg
    16321 acgaatcgtt aagggggcca gccaggcacc gtacattgcg aagccaaaga ccagcatcag
    16381 cgaggtgaaa tggataaatc gcagcgcgat ccaggtaaac gccagcatga tttatttcac
    16441 gctaaaggtg tagtgtcctt tcgttttatg cccgtccacc gaaacaacat gccagtcaac
    16501 gatgtaggtt ccgggtttca gcgaatcggc cagtggaaca atcagttgct tttggtcctg
    16561 ctcatttcgc ttcgccggta atgttttaat attttcgttt tttggccccg tgatttttgc
    16621 accactgaat cctgtttcaa caccttccga gaagtttaaa gtgattgcct gcggtgcagc
    16681 tgtcacttgc gcgtttgccg caggatactg atgcgttaaa tgcgcatgtg cccagacgga
    16741 aggtgtcacc aacgaagtgg tcaggattgc cagcgcgtag cgaagggagc gtgcagttga
    16801 agtcatatta tctattcctt tttgtaataa cttttttaca gagcataacc ttgtctaatg
    16861 tctgagtcga ggatcatcaa ttccggcttg ccatcctggc tcactcttag taacttttgc
    16921 ccgcgaatga tgaggagatt aagaatgctg aagaatctgg ctaaactgga tcaaacagaa
    16981 atggataaag tgaatgtcga tttggcggcg gccggggtgg catttaaaga acgctacaat
    17041 atgccggtga tcgctgaagc ggttgaacgt gaacagcctg aacatttgcg cagctggttt
    17101 cgcgagcggc ttattgccca ccgtttggct tcggtcaatc tgtcacgttt accttacgag
    17161 cccaaactta aataaaactt atacagagtt acactttctt acataacgcc tgctaaatta
    17221 tgagtatttt ctaaaccgca cccataattt gcagtcattt tgaaaaggaa gtcattatgt
    17281 cgttctggaa agttgcagca gcgcaatatg agcctcgtaa aacctcactc accgaacagg
    17341 tagctcatca tctggagttt gtgagggccg ccgccaggca acaatgtcag ctgttagttt
    17401 ttccatcgct ttctttactg gggtgtgatt attcgcgacg tgcccttcct gccccacccg
    17461 atctctcact gttagacccg ctttgttatg ccgcaacgac ctggcgaatg accatcattg
    17521 ccggccttcc cgttgaatat aacgatcgct ttattcgtgg aattgcggtg ttcgcaccgt
    17581 ggcgaaaaac gcctgggatc taccatcaga gtcatggtgc atgtctgggt cgtcgttcca
    17641 gaaccatcac ggtggtcgat gaacaaccgg aaggtatgga tatggacccg acctgttcac
    17701 tgtttactac agggcaatgt ctgggagaac ccgacctgct ggcgtcggcc cgtcgcttac
    17761 aatttttttc acatcagtac tcgattgccg tgctgatggc aaatgcccgt ggtaacagtg
    17821 cactatggga tgaatatggt cgtctcatcg ttcgcgccga tcgtggttca ttattgttag
    17881 tcggtcagcg ttcatcacag ggttggcaag gcgatatcat tccattacgc taggcttttt
    17941 cggcctggag catgccatgt tgcgcattat cgatacagaa acctgcggtt tgcagggagg
    18001 gatcgttgag attgcctctg ttgatgtcat tgacggaaaa atcgtcaacc ccatgagcca
    18061 cctggtgcgc cccgatcgtc ctattagtcc acaagcgatg gcgattcatc gcatcaccga
    18121 agccatggtc gccgataaac cgtggattga agatgtgatc ccacactatt acggtagtga
    18181 atggtatgtc gcgcataacg ccagctttga ccgccgcgta ctgcctgaga tgcccggtga
    18241 gtggatttgc actatgaaac tggcccgtcg tttgtggcct gggatcaagt acagcaatat
    18301 ggcgttatat aaaacacgca agctcaatgt acagacgccg ccgggcctgc atcatcaccg
    18361 cgcgttgtat gactgttata tcaccgccgc gttgcttatc gatattatga acacctccgg
    18421 ctggacggca gaacagatgg ccgatatcac cggacgtccg tcgttgatga cgaccttcac
    18481 ctttggcaaa taccgtggca aagcggtttc cgacgttgcc gaacgcgatc cgggctatct
    18541 gcgctggtta tttaataacc tggacagcat gagcccggag ctgcgtttaa cactgaaaca
    18601 ttatctggaa aatacttagt ccgcaggcgt agcgggtaat gttccctgcg ccagcgcgac
    18661 cagaaaagca tattccatcg ctacgccttc gtacgattta aagcgaccag atttaccgcc
    18721 atggcctgag tccatgtcgg tacagagcaa taaaagatgg tcatcggttt tcagctcgcg
    18781 caatttagcg acccattttg ccggttccca atattgcacc tgagaatcgt gcaaaccggt
    18841 cgttaccagt aaatgcggat aagcctgtgc ggtgacgttg tcatacgggc tgtagctttt
    18901 catgtactcg taatattgcg gatcctgcgg gttaccccac tcttcaaact caccagtggt
    18961 aagaggaatt gattcatcaa gcatcgttgt tacaacatca acaaacggta cctgggcgat
    19021 aacgccgtgg aataattccg ggcgttgatt aattgcaacg cccatcaaca tgccccccgc
    19081 actcccgccc atcgcataac aaagcgaagg agagccatag cccagtttta acaatgcatc
    19141 gcaggcatca agataatcat taaacgtatt tttcttcttc agaaattttc cgtcttcgta
    19201 ccattgttgc cccagctcac caccgccgcg aacatggaca atggcgtaga caaagccacg
    19261 atctaacaaa ctcaagcggc taaaactgaa atcggcatca atacttgcgc cgtaagaacc
    19321 atagccatac accagcaacg ggttgtgtcc tttgcgaaaa tgtttgcgat ggtagaccaa
    19381 cgaaaccgga acttcgacgc catcacgggc gactatccac aggtgttcac tgcggtaatt
    19441 cgccgcatca aaaccgggca cttctgtttg ttttaataca cgacgctcac cggtatccat
    19501 atccagttca aacaaagtgt ctggtgtggt catggaagaa taaccataac gcaatcgcgc
    19561 ggtttcaggt tctggattgt aggcaatcca ggtcacatag gctggatcat caaaggcaat
    19621 accaatgact tcccgggtct tgcggttaat ttggcgcaaa ctggttaacc cgcgctgacg
    19681 ctcttcaacc accagccagt cggtaaacag cgtaaaccct tccagcatga tgttttcgcg
    19741 tggcggaatt aactcttccc actgttgctc atcacgcata cgggtacggt ataagccaaa
    19801 gtttttgccg tggcggttgg aacgcagata aaaacgatgc tggtagtgat caaggctgta
    19861 ttcgtgatct ttgcggcgcg gcagaaaaac aaacggctcg gcatcggcca tttccgcgtc
    19921 cagcaggcga acttcactgg tggtggcgct ggccaaatga atgactacat agtgcttcga
    19981 ggtcgtttta tgcaggctga cgtaataggt atcgtctttt tcttcgtaga tcagtttatc
    20041 ttgcgatgct ggcgtaccga tggcgtgacg ccagacctga taaggcagca gcgtcaccgg
    20101 atgcttgcga acatagtaga aagtccagga gtcatttgcc cagacaaagc tgggttcaac
    20161 gttatccagc agttccgggt accagttacc tgtttccaga ttacgaaaac gaatgccgta
    20221 ctggcgtcgg gaaagaaaat cttctgccag cgccataatg gtgttatcgg gcgtaatcgc
    20281 cattccgccc atcgaataaa actcactatg agccgcgcgc ttgttggcat cgagcaatat
    20341 ttcccactcg tcccactctt cactgaacgc cgattgacgc tggtagatag catattcaca
    20401 gcctggttca taaatatgcc gatagcggta gccatttttg atgtagggcg cagaaacttc
    20461 tcgttgcgga atgcggtcga tgatttcctt taagatgcga tcctgcaagg cttgttgtga
    20521 ggccatcacc cgatgaccgt aactattttc ttgttgcagg tagtccagga cttctggctg
    20581 agaacgcgta tcgtcccgca gccagtagta attatcgatg cgcgtatcgc catgaagcgt
    20641 catggcgtgg ggaatgcggg cggcttttgg tagcatgtta ttgttctttc tggttgaaac
    20701 atcctataag ggtggcaaaa ctcaccgggg atgcaagcga aacaggggag tcattgctta
    20761 gatgatgaca ggtaatggcg cggatatcga atgttatgca gacagagaaa atcagtctgt
    20821 tcaaatggct gtgcggttct ggatagcccg aaatattcaa cttcagacta tccagagagc
    20881 ggaattattc cgccaaagtg cgtttttgct gttcgagatc gcgttcaatg ccgtcacgaa
    20941 catcctgggg gattttcagc gcgtcaccca gtgcattcag gtaactgcgt tccataaaat
    21001 ggtcaatatc aatagccgcg caactcagga aatagatttc cagcgcctct tcttcattgc
    21061 ggaccccggt agccaggcgt tgtggatcca gcggttgttc gattgcctgc tcaatgagta
    21121 cacgcccctg ctcttccacg ccggcttcac gcaattgctg gtcgattgcc gcacgttctt
    21181 tggcatcaat atgaccatca cttttagcgg caaagaccag cgcaaggatc aaacgttctg
    21241 tacgctcatc cagcggcgta ctttgcgcgc caaactgcgg ttcgtcctga tgcgccgcgc
    21301 gaattttatc tttgtattta ttccacagca ccgtacccgc taccgctccg ccgccaacca
    21361 gtaacgcgtt ggtgccatat ttcgtaagaa gtttacgtgc tgatttattt gcgaccagca
    21421 gcccagccag tccgcctaat gcgcctggca ctaacagttt gcccaatccc tgatccgcag
    21481 acgaggaggt agaagaactg ctttgcccaa gcagggattg cagttgattt aaccagttag
    21541 ccatatttgc tcctcaataa ccatttttag tgcctgtagc atagcggatg aggatgtcag
    21601 aaaatgtata tcgagacaaa agatgcgcaa ataagcgggg cggtaggccg ttattcaaag
    21661 aaattcgcgc caggtaaggt atttactgta tatcaggtga taagggtatt ttaggtgaaa
    21721 gtagtttgtc gttttgagtg gcattgtttg atgctgattg cccgatgcga cgctgttgcg
    21781 tcttctcagg cctacgattg tggtgcgttc tggggcggac aaggcactcg cgccgcatcc
    21841 gccaacggtc aatcgaatta tcagaagcca ttaacgccgc tgatattccg ccattcccgc
    21901 tttgcagtcc acgctgacct ggtaatgaat atcggcactt ttaccacgca cggttagcgg
    21961 tactgaccat ttatcatctt taccctgaat gtcctgcaaa ctgacccaag ccacgggatc
    22021 ggcctgaccg acaatttttt gatcatctgc ccagcgcgcc acgcgatttt gttgataatc
    22081 acgttttacg ctcgcggcaa ttccggcagc atccagacct tcacactttg ggaaagtgac
    22141 cgacttgctg gtttcattat tggcagcgaa aactgatgcg caggcagaaa ccaacaacag
    22201 ccctaaaaac gcccctcttt ttttcatgtt tttctccata gcacaatgat tcaggagaaa
    22261 gcatggtaca aattgtcagg agcgcaagtt gcttcaggcc gcgtgtgagg catcttcctc
    22321 ttcggattca gcaccgacga cctgtgggga cggtaatttg ccggttttca gaatggtgct
    22381 cagcacatct ttttgttctg ccagccatag cgaaagggct tcacgttgct cgtcttccat
    22441 ttgcactgga gaatttgtca gccaggtgtc aagcagatcc gccgtatcaa gcattttgtc
    22501 ataagcatcg gcttcctttt tgctggtaaa cgacattttc tcttcgccct cacgaatgac
    22561 tacgtattta acttcaaccg ccatttgcag cctctcataa taactgtgat tttatacagt
    22621 atatttcttt tcggttgaga aatcaacatc agcaataaag acatacgcaa acgttttcgt
    22681 ttatactgcg cgcggaatta atcaggggat attcgttatg acgttattag gcactgcgct
    22741 gcgtccggca gcaactcgcg tgatgttatt aggctccggt gaactgggta aagaagtggc
    22801 aatcgagtgt cagcgtctcg gcgtagaggt gattgccgtc gatcgctatt ccgacgcacc
    22861 agccatgcat gtcgcgcatc gctcccatgt cattaatatg cttgatggtg atgcattacg
    22921 ccgtgtggtt gaactggaaa aaccacatta tatcgtgccg gagatcgaag ctattgccac
    22981 cgatatgctg atccaacttg aagaggaagg actgaatgtt gtcccctgcg ctcgcgcaac
    23041 gaaattaacg atgaatcgcg agggtatccg tcgcctggcg gcagaagagc tgcagctgcc
    23101 cacttccact tatcgttttg ccgatagcga aagccttttc cgcgaggcgg ttgccgccat
    23161 tggctatccc tgcattgtaa aaccggtgat gagctcttcc ggcaaggggc agacgtttat
    23221 tcgctctgca gagcaacttg ctcaggcatg ggaatacgct cagcaaggcg gtcgcgccgg
    23281 agcgggccgc gtaattgttg aaggcgtcgt taagtttgac ttcgaaatta ccctgctaac
    23341 cgtcagcgcg gtggatggcg tccatttctg tgcaccagta ggtcatcgcc aggaagatgg
    23401 cgactaccgt gaatcctggc aaccacagca aatgagcccg cttgcccttg aacgtgcgca
    23461 ggagattgcc cgtaaagtgg tgctggcact gggcggttat ggtctgtttg gtgtcgagct
    23521 gtttgtctgt ggtgatgagg tgattttcag tgaggtctcc cctcgtccac atgataccgg
    23581 gatggtgacg ttaatttctc aagatctctc agagtttgcc ctgcatgtac gtgccttcct
    23641 cggacttcct gttggcggga tccgtcagta tggtcctgca gcttctgccg ttattctgcc
    23701 acaactgacc agtcagaatg tcacgtttga taatgtgcag aatgccgtag gcgcagattt
    23761 gcagattcgt ttatttggta agccggaaat tgatggcagc cgtcgtctgg gtgttgcgct
    23821 ggcgaccgct gagagtgttg ttgacgccat tgaacgcgcg aagcacgccg ccggacaggt
    23881 aaaagtacag ggttaaaccc ggcaaaaaaa cgctacaaaa atgcccgatc gtagatcggg
    23941 cgttttgact tttacagctt agcgccttct acagcttcac gcgccagctt agtaatgcgg
    24001 tcgtaatcgc ccgcttccag cgcatctgcc ggaaccagcc aggaaccacc gatgcacagc
    24061 acgcttttca gcgccaggta gtcacggtag ttagccggag aaataccacc cgtcgggcag
    24121 aaacggacct gggagaacgg acccgcgatc gcctgcaggg ctttcacgcc gccgttagct
    24181 tcagccggga agaatttgaa ctctttcaaa ccgtagtcca tacccagcat cagttcggaa
    24241 acagtgctga tccccggaat tagaggaata gtcccttcgg tagcagcttt cagcagcggc
    24301 tcggtcagac ccgggctaat tgcgaactgt gcacccgctt cagtgacttc tgccagctgc
    24361 tgtggattca gcaccgtacc ggcacccaca atcgcttcag gcacttcttt ggcgatagca
    24421 cggatagcgt caactgcaca ctcggtacgc agagtcactt ccagaacgcg caccccacca
    24481 gcaaccaacg cttttgccat cggcactgcg tgttccagtt tttttaccac gataaccggt
    24541 acaaccgggc cggtggtcag gattgattct gcacttgttt tccagttttt catcagagtt
    24601 ttctctcgcc tgattacaaa tttgtcgtct taaaaagtga tacaggttgc gccctgttcg
    24661 gcaccggaca gtttttcacg caaggcgctg aataattcac gtcctgtccc cacgcgtgac
    24721 gcgctcaggt caggaatgtg cggttcgcga gcagccagtt ccgcttcgtc taccagcagc
    24781 gtcagttcgc ctgtctgtcc attcacacga atgatgtccc cgtcgcgcac ttttgccagc
    24841 agcccgccat cgtaggcttc tggtgttacg tggatagctg acggcacttt acctgaagcg
    24901 ccggagagtc gtccatcggt aactaacgca attttgaaac accggtccaa taatacacca
    24961 agtggcggca tgagtttatg taattctggc attccgttcg cttttggccc ctgatgacgg
    25021 acaacaacga cacaatcgcg gtccagcaaa cccgcttcaa aggccggcat aacgtcatgc
    25081 tggctttcaa aaacaaccgc tggcgcttca atcacctggt tctcaaccgg cacggcagag
    25141 gttttcataa ccgcacggcc caggttaccg cttaacactt ttgtcccacc atgatgagag
    25201 aaaggttgtt cgaaggaagc gatcacattg ctgtcgagtg atttttccgc cccttcccgc
    25261 cagtccagtt caccattatt cagccatggt tcaagggtat aacgagacag accaaagcct
    25321 gccaccgtat tgacatcttc atgcagcagg cctgctttga gcagttcacg caccagaacc
    25381 ggtacgccac ctgccgcctg gaagtggtta atatcggccg gaccgttcgg gtagagacgt
    25441 gccatcagcg gtacaacatc agaaaggtcg gagaagtcat cccagttaat ctgaataccg
    25501 gccgcgcgcg ccatcgccac caggtgcatg gtgtggttag tggaaccacc ggtcgccagc
    25561 agagcaacaa taccgttcac caccactttc tcatcgatca tcttaccgat cggcatccat
    25621 tcattaccat taccggtcat gcgtgtaacc tgacgcgcag cggcagcggt caaagcatcg
    25681 cgcagcggag aatccggatg aacaaaagaa gagcctggca actgcatccc cataaattcc
    25741 accaccatct ggttggtgtt ggcagtaccg tagaaagtac atgttcccgg cgcatggtaa
    25801 gacgcggctt ctgactccag taaggccatg cggtccactt taccttcggc ataaagctgg
    25861 cgaatacgca ctttttcttt atttggcaaa ccgcttgcca tcggtccaga cggcacaaac
    25921 accgcaggca aatgaccaaa cgacagggct gccatcgtca gacccgggac aatcttgtcg
    25981 cacacaccga ggaacagagc accatcaaac atgttatggg acagcccgac cgccgcagac
    26041 atcgctatca cttcgcggct tagcagcgac aattccattc catcctgccc ctgggtgaca
    26101 ccatcacaca tcgccggaac accgcccgca acctgaccaa ccgcattcgc ttcatgcagg
    26161 gctttacgaa tgatttctgg atagtgttca taaggctggt gcgcggagag catgtcgtta
    26221 taggaggtga tgatggcgat attgttacgc aacatgcttt tcaaagaggc tttgtcttct
    26281 ggctggcagg cagcgaaacc gtgtgccagg ttaccgcatg ccaactgcga acgatgaacg
    26341 gtcgaagttt tcgcttgttc tatccgggcg agataagcag agcgagtctc gcgcgaacgt
    26401 tcaatgattc gatttgttac gcgtaacaat tgtggattca taaaggctcc tgaaattgag
    26461 ttgtcagagc aggatgattc acaacgcgtt tcattcagag gatttatgac tgaaacgcct
    26521 gtaaccggag ctcatagggc aaaaacgttt cagtcagtgt aataaaaaaa gcctcgtggg
    26581 tgaatccgca cgaggcctga aagtgtaaaa attgttctac aatctgtgca agatcatgtt
    26641 accggtaaaa taaccataaa ggataagcgc agatattact caaactcatt ccaggaacga
    26701 ccatcacggg taatcatcgc caccgaggca acgggtcccc aggttccggc ctgatacggt
    26761 ttcggcgcat cattgtccat cgcccacgcc tcagtaatgg agtctaccca tttccaggct
    26821 tcttccactt cgtcgcgacg aacaaacagt gcctgaatac cacgcatggt ttccagcagc
    26881 aaacgttcat aggcatccgc cagatgcgtc tgattaaagg tttctgaata gctcagatcc
    26941 agcttggtga tttgcaggtt atgtttgtgg tcaaggccag gaactttatt cagtacctgg
    27001 atatccacgc cttcatcagg ttgcagacgg atagtcagtt tattctgcgg cagatcctgc
    27061 cacgattctt taaacagatt cagttcaggt gttttgaaat agaccacgac ttcagaacat
    27121 ttggtcggca gacgtttacc agtacgcagg tagaatggca caccggccca gcgccagtta
    27181 tcaatgtcga cgcggatcgc tacgaaggtt tctgtattgc tgctcttgtt cgcgccctct
    27241 tcttccagat atcccggcac ttttttgccc tgggcgaagc ccgcagtata ttgcccgcgt
    27301 acggtttttt cgcgtacgtt ggagcggtcg atgcggcgca gagacttcag tactttcact
    27361 ttttcatcgc ggatgctgtc tgcgctcagg tcagacggcg gagacatcgc aatcatgcag
    27421 agaatttgca gcaggtggtt ttggatcata tcgcgcatct gaccggcttt atcaaaatag
    27481 ccccagcgcc cttcgatccc cacttcttct gccacggtaa tctcaacatg atcaatggtg
    27541 cgattgtccc agttattcac aaacagggag ttagcaaaac gcagcgccaa caggttcagc
    27601 accgtttctt taccaagata gtggtcgata cggtaaacct ggcactcctc gaagtattcg
    27661 ccaacctgat cattgatttc ctgcgaggtc gccagcgacg tccccagcgg tttctccata
    27721 actacgcgtg ccggtttagc attcagtttt gcctcaccaa gccctttgca aattgcgcca
    27781 aaagtgctgg gcggcatggc aaagtagtta atggtgatac gatttttttg atccagcatc
    27841 gcgccgagac ggctgaatgc agcagtgtca ttgacatcga gattacaaaa atccagacgt
    27901 gcactcaggg tgtcccataa accttcatca atagtttctt tcatgaaagt ttcgagcgcc
    27961 tcacggacaa ctttggtata agccgcttta tcccagtcag cacgccctac gccgataatc
    28021 cgggtgtccg ggttgagctg accggctttt tccagttgat acagggaagg cagcaattta
    28081 cgacgcgcaa ggtcgccttt cgcgccgaaa atgaccaggt cacaggcctg ggctgtttgc
    28141 gttaccgcca tgtcattctc cttaagttaa ctaacccggt acttaagcca gggtatactt
    28201 gtaattttct tacggtgcac tgtactgctt ttacgagctt gcgaaaactg taaacgctta
    28261 tccacccgtg cgattacggg aaaagcgcgc aaagtgcggc aaaactgata aaaaaatcat
    28321 cgttttccat tcggtaaaaa tctgacactg atcatgttat gaaaaaaaat aacaactttt
    28381 ttatctgctt ttgtcattaa cacggcacac aggcgtaata tctgacaaaa ctgcatttcg
    28441 atttctttca gtgcggaaat cgtcattacc cgtgagtctc tttacatcat gaatatgctg
    28501 gaaaaaatcc agtctcagct ggaacatttg agcaaatcag agcgcaaagt tgccgaggtc
    28561 attctggctt cgcccgataa cgcgatccat tcgagtattg ctgctatggc actggaagcc
    28621 aatgttagcg aaccgacggt gaatcgtttc tgtcgcagca tggacacgcg cggttttcct
    28681 gattttaaac ttcatctggc acagagtctg gcgaatggca ctccctatgt taatcgcaac
    28741 gtcaatgaag atgacagcgt tgaatcatac acagggaaaa tatttgagtc cgcaatggca
    28801 acgctcgatc atgtccgtca ttcactggat aaatctgcca tcaaccgcgc cgtcgacttg
    28861 ctcactcagg caaaaaaaat cgcctttttc ggattaggct cttcagccgc cgttgcccac
    28921 gatgcgatga ataagttctt tcgttttaat gttccggtgg tgtactccga tgatattgtg
    28981 ctgcaacgca tgagttgtat gaattgtagc gacggagacg tggtggtgct gatttctcac
    29041 actggaagaa caaaaaatct ggtcgagctg gcgcagctgg ctcgcgaaaa cgacgcaatg
    29101 gtgattgccc tcacctctgc gggtaccccg ctcgcccggg aagcaacgct ggcaattacc
    29161 ctcgacgtac cggaagatac tgacatttat atgcccatgg tttctcgact tgcacagctg
    29221 accgtgatag atgtgctggc gacaggattt actttgcgac gcggtgcaaa attcagagat
    29281 aacttgaagc gggtcaagga agcgctgaag gaatcgcgtt ttgataagca gttacttaat
    29341 ttaagtgacg atcgctaaaa acgactgtca ctgtcctaat cttatacgac atccgaatga
    29401 gattaattta tcgccatcgc ggcgttattt cattcggatt tcatgttcaa gcaacacctg
    29461 gttgtttcag tcaacggagt attacatgtc cagaaggctt cgcagaacaa aaatcgttac
    29521 cacgttaggc ccagcaacag atcgcgataa taatcttgaa aaagttatcg cggcgggtgc
    29581 caacgttgta cgtatgaact tttctcacgg ctcgcctgaa gatcacaaaa tgcgcgcgga
    29641 taaagttcgt gagattgccg caaaactggg gcgtcatgtg gctattctgg gtgacctcca
    29701 ggggcccaaa atccgtgtat ccacctttaa agaaggcaaa gttttcctca atattgggga
    29761 taaattcctg ctcgacgcca acctgggtaa aggtgaaggc gacaaagaaa aagtcggtat
    29821 cgactacaaa ggcctgcctg ctgacgtcgt gcctggtgac atcctgctgc tggacgatgg
    29881 tcgcgtccag ttaaaagtac tggaagttca gggcatgaaa gtgttcaccg aagtcaccgt
    29941 cggtggtccc ctctccaaca ataaaggtat caacaaactt ggcggcggtt tgtcggctga
    30001 agcgctgacc gaaaaagaca aagcagacat taagactgcg gcgttgattg gcgtagatta
    30061 cctggctgtc tccttcccac gctgtggcga agatctgaac tatgcccgtc ggctggcacg
    30121 cgatgcagga tgtgatgcga aaattgttgc caaggttgaa cgtgcggaag ccgtttgcag
    30181 ccaggatgca atggatgaca tcatcctcgc ctctgacgtg gtaatggttg cacgtggcga
    30241 cctcggtgtg gaaattggcg acccggaact ggtcggcatt cagaaagcgt taatccgtcg
    30301 tgcgcgtcag ctaaaccgag cggtaatcac ggcgacccag atgatggagt caatgattac
    30361 taacccgatg ccgacgcgtg cagaagtcat ggacgtagca aacgccgttc tggatggtac
    30421 tgacgctgtg atgctgtctg cagaaactgc cgctgggcag tatccgtcag aaactgttgc
    30481 agccatggcg cgcgtttgcc tgggtgcgga aaaaatcccg agcatcaacg tttctaaaca
    30541 ccgtctggac gttcagttcg acaatgtgga agaagctatt gccatgtcag caatgtacgc
    30601 agctaaccac ctgaaaggcg ttacggcgat cattaccatg accgaatcgg gtcgtaccgc
    30661 gctgatgacc tcccgtatca gctctggtct accaattttc gccatgtcgc gccatgaacg
    30721 tacgctgaac ctgactgctc tctatcgtgg cgttacgccg gtgcactttg atagcgctaa
    30781 tgacggcgta gcagctgcca gcgaagcggt taatctgctg cgcgataaag gttacttgat
    30841 gtctggtgac ctggtgattg tcacccaggg cgacgtgatg agtaccgtgg gttctactaa
    30901 taccacgcgt attttaacgg tagagtaagt acgttgccgg atgcggcgaa aacgccacat
    30961 ccggcctaca gttcaatgat agttcaacag atttcgaata ttctgaagca aacttgaact
    31021 tatcatcagg cgaaggcctc tcctcgcgag aggctttttt atttgatggg ataaagatct
    31081 ttgcgcttat acggctggat ttcgcccggt ttgcgagttt tcagcaattt tagtatccag
    31141 gtgtattgtt ctggtcgcgg accaacaaaa atctcgactt cttcattcat ccgccgcgca
    31201 atcgtatgat catccgcctc taacagatca tccatcggtg ggcgcacctg aatcgtcaga
    31261 cggtgcgtct tgccatcata aatcggaaac agcggaacaa cgcgcgcacg gcacactttc
    31321 atcaaacgac caatcgcggg caacgtcgct ttataggtgg caaagaaatc aacaaattcg
    31381 ctgtgctcag ggccatgatc ctgatcgggt aaataatatc cccagtagcc ctgacgtacc
    31441 gactggatga atggtttaat accatcattt ctcgcatgca gacgaccgcc aaaacgacga
    31501 cgcaccgtgt tccagacata atcaaaaacc gggttgccct gattatggaa catcgctgcc
    31561 attttctgcc cttgcgaggc catcagcatg gcaggaatat cgacggccca accgtgcggc
    31621 accagaaaga taactttctc gttattacgc cgcatctctt caatgatctc cagcccttgc
    31681 cagtcaacgc gcggctgaat tttctccggc ccgcgtattg ccaactcagc catcattgcc
    31741 atcgcttgcg gcgcggtggc aaacatctca tcaacaatcg cttcgcgttc agcttcacta
    31801 cgttctggaa agcagagcga cagattgatt aacgcacgac ggcgtgagct ttttcccagt
    31861 cgtccggcaa aacgtcccag ccgtgccaga atgggatcac ggaactttgg cggcgttaaa
    31921 gcgatacccg ccatcgctgc tacgcccagc catgctcccc agtagcgcgg gtggcgaaag
    31981 gatttatcaa actcaggaat gtattcgcta ttattttttt tcgtttccat gcttttccag
    32041 tttcggataa ggcaaaaatc aatctggtga tagtgtagcg gcgcaacttg ccccgcacca
    32101 aataaaaaaa gccggtactg actgcgtacc ggctgcgaat ggatgttaat taatcaaacc
    32161 gtagctgcgg cacaatctct ttggcctgcg ccaggaattc gcgacgatcg gagccggtca
    32221 gcccttcggt acgcggcagt tttgccgtca gcgggtttac ggcctgctgg tttatccata
    32281 cttcatagtg cagatgtggc ccggttgaac gtccggtatt accggaaagc gcgatacggt
    32341 cgccacgttt caccttctgt cccggtttca ccagaatctt gcgcaagtgc atataacgcg
    32401 tggtgtagct gcgaccatga cgaatagcca cataataacc tgctgcgcca ctgcgtttgg
    32461 caaccaccac ttcaccgtca cccactgaaa gcactggcgt accctgcggc atggcgaaat
    32521 caacacctct gtgtggtgca acgcgaccgg tcacaggatt agtacgacgc gggttaaagt
    32581 tagacgagat acggaactgt ttcgccgtcg ggaatcgcaa gaatcctttc gccagaccag
    32641 taccgttacg atcgtagaat ttgccatctt cagcgcggat agcgtaataa tctttacctt
    32701 ctgaacgcaa acgtacgccc agcagctggc tttgctcacg tttaccatca agcatttctc
    32761 gagacattaa taccgcaaat tcatcgcctt ttttcagttt gcggaaatcc atttgccact
    32821 gcatggcttt aatcactgcg ctcacttcgg cgctggttaa accggcgttt ctggcgctgg
    32881 caacaaagct ccccccgacg gtacctttca gcagattgtt aacccactct ccttgctgca
    32941 tttcgctggt cattttaaaa ccgttagcgg cagtacggtc ataggttcgg gtttcacgac
    33001 gagacacttc ccaggtgagg cgctgcagtt cgccgtccgc ggttaatgtc caggagagtt
    33061 gttgaccgat tttcaggtta cgcaattctt tgtcggcagc agccagttgg gtgatatcac
    33121 ccatatcaat accatactga ttgagaatgc tgcttagcgt atcgccagtg gaaacaacat
    33181 attcatgcac gcccgcttca ccggcgattt tgtcatccag ttcgtcctgg ggaatggctt
    33241 catcttcttg tgcagcttga tcaatcggct cactggcttc aggtaagagc gaacgaattt
    33301 cgttctgttc cagctcaatg gttttgacaa ttggcgtggc atcgcggtga taaacatagg
    33361 gccgccagac agcgacggcc agagtaagaa cggtgagcga ccccaacata acgcggtgtg
    33421 gtcgcggtaa attattaaac gccagggcga cagagcgggc tatctgttgc acgtaatcac
    33481 ttcctcatta atctcctttc aggcagctcg catactggtt ggctaattga ctcaggaatt
    33541 ctgaatagct tgttttaccc agtttgatat tcgtccccag gggatccaac gttcccatac
    33601 gaacggatgt ccctcgtgcg acgctctcaa cgaccgctgg cctgaactgt ggctcagcaa
    33661 aaacgcaggt tgctttttgc tcaaccaact gtgttcttat ttcatgtaaa cgctgcgcgc
    33721 caggttgaat ctcagggtta acggtaaaat gaccaagcgg tgtcagtcca aactgttttt
    33781 cgaaatagcc gtaagcatcg tgaaaaacga aataaccttt ccccttgagc ggcgcgagct
    33841 cgttaccaac ctgcgtttcg gttgaggcta attgtgcctc aaaatccttc aggttggcgt
    33901 caagtttggc tcgactttgc ggcataagtt ccactaattt tccatggatt gcaaccgctg
    33961 tagcccgcgc tatctctggg gaaagccaaa gatgcatgtt gaaatcgccg tgatggtgat
    34021 cttcgtcact tttttccgcg tggtcgtgat catcatcatc gccgtgaata cttttcatca
    34081 gtagcggttt cacatcttca agctgcgcaa tcgttacctg cttcgcttct ggtaatttgc
    34141 ttaccggttt ttgcataaac gcttccatct ccgggccaac ccaaacgact aagtccgcgt
    34201 tctgtaagcg ttttacatcc gatggacgca gtgaataatc atgttctgaa gcgccgtcag
    34261 gcagtaaaac ctccgtttct gttacaccat cagcaatggc agaagcgatg aacccaacgg
    34321 gtttaagcga agcgacaacg gcggcatctg cggcctgtgt tgcacctccc cagagagcgg
    34381 cggataatgc tgcgaaaaga agcgtttttt tatgtaacat aatgcgacca atcatcgtaa
    34441 tgaatatgag aagtgtgata ttataacatt tcatgactac tgcaagacta aaattaacat
    34501 gacaagtctg gtttccctgg aaaatgtctc ggtttctttt ggccaacgcc gcgtcctctc
    34561 tgatgtgtcg ctggaactta aacctggaaa aattttgact ttacttgggc caaatggcgc
    34621 aggtaagtcg acactggtac gggtagtgct cgggctggta acacccgatg aaggggttat
    34681 caagcgcaac ggaaaactgc gcatcggcta tgtaccgcag aagctgtatc tcgacaccac
    34741 gttgccactg accgtaaacc gttttttacg cttacgccct ggtacacata aagaagatat
    34801 tttgcctgca ctgaaacgtg tccaggccgg gcacctgatt aacgcaccga tgcaaaagct
    34861 ctctggtggc gaaacgcagc gtgtactatt agcgcgagca ttgttaaatc gcccgcaatt
    34921 attagtgctg gatgaaccca ctcaaggcgt ggatgtaaat ggccaggtgg cgttatatga
    34981 ccttattgac caactgcgtc gcgaactgga ttgtggcgtt ttaatggtct ctcacgatct
    35041 gcatctggta atggcaaaaa ccgatgaagt gctgtgtctg aatcaccaca tttgttgttc
    35101 cggcacaccg gaagttgttt ccctacatcc ggagtttatt tctatgtttg gtcctcgtgg
    35161 tgctgaacaa ctgggtatct atcgccatca tcataatcat cgtcacgatt tacagggacg
    35221 aattgttttg cgtcggggaa atgatcgctc atgattgaat tattatttcc cggttggtta
    35281 gccgggatca tgctcgcctg tgccgcgggt ccgctgggtt cgtttgtagt ctggcgtcgt
    35341 atgtcttatt tcggtgatac gctggctcat gcctcattac ttggcgtcgc gtttggtttg
    35401 ttgctggacg tgaatccatt ctatgcggta attgccgtta cgctgctcct ggcgggcggt
    35461 ctggtatggc tggagaagcg tccacagctg gcgattgaca cgttattagg gattatggcg
    35521 cacagtgccc tgtcgctggg cctggtggtc gttagtctga tgtctaacat tcgtgttgat
    35581 ttgatggctt acctgttcgg tgatttgctg gcagtgacgc cagaagatct catcgctatt
    35641 gcgattggcg tggtcatcgt ggtggctatt ttgttctggc aatggcgcaa tttgctgtcg
    35701 atgacgatta gcccggatct ggcgtttgtt gatggtgtga aattacagcg cgtgaaattg
    35761 ttgttgatgc tggtgacggc attgacgatt ggtgtagcga tgaaatttgt tggcgcgttg
    35821 attattactt cactgctgat tattcctgca gctactgcgc gtcgctttgc ccgtacgccg
    35881 gaacagatgg ctggtgtcgc tgttttggtg gggatggtgg cagtaactgg cggtttaacc
    35941 ttttccgcgt tttacgatac gccggcgggt ccgtcggtgg tgctatgtgc ggcactgtta
    36001 tttattctca gtatgatgaa aaagcaggcc agctaatcta tcgctgaaca catttgtcgg
    36061 atgcggcgcg agcgccttat ccgacctacg gttcgctatc tctggctggc ctgataagac
    36121 gcgaacagtg tcgcatcaga cacactgcca gtgtcggatg cggcgcgagc gaccaatccg
    36181 acttacggca tttctggcgg cgttatgcca aagtgattcc acgcccgcgt cgtcgccata
    36241 cgcccacgcg gtgtacgctg caaaaagcct tgctgaatca aataaggttc cagcacatcc
    36301 tcaatggttt cacgttcttc gccaatggct gccgcaaggt tatccagacc taccggccca
    36361 ccaaagaact tatcgattac cgccagcaat aacttgcggt ccatataatc gaaaccttca
    36421 gcatcgacat tcaacatatc caacgcctga gcagcgatat ctgccgagat ggtgccatcg
    36481 tgcttcactt cggcgaaatc acgcactcga cgcagcagac ggttggcaat gcgcggcgta
    36541 ccgcgagctc gacgagcaac ttccagcgcg ccgtcatcac tcatctcaag ccccataaag
    36601 cgtgcgctgc gactgacgat atattgcaga tccggcacct gataaaactc cagacgctgc
    36661 acaataccaa aacgatcgcg caacggtgat gtcagcgaac ctgcgcgcgt ggttgcacca
    36721 atcagggtaa acggcggcaa atcaatttta atggagcgtg ccgccggacc ttcaccaatc
    36781 atgatatcca gttggtagtc ttccattgcc ggatacaaca cctcttccac cactggcgaa
    36841 agacggtgga tctcatcaat aaacaacacg tcatgcggtt caaggttagt gagcatcgct
    36901 gccagatcgc ccgccttttc cagcaccgga ccagaagtcg tgcgtaaatt aacgcccatt
    36961 tcattggcga cgatgttggc aagcgtagtt ttacccaacc ccggaggacc gaaaatcaac
    37021 agatgatcga gggcatcgcc gcgcagtttc gctgctttga tgaaaatctc catctgtgaa
    37081 cgaacctgcg gctgaccaac atactcttcc agtaatttag ggcgaatggc gcgatctgcc
    37141 acatcttccg gcaaagtggt accggcagaa atcagacggt ctgcttcaat catcctttac
    37201 ctcataacgc ggcgcgtagg gcttcgcgaa ttaatgtttc actgctggcg tcagggcgag
    37261 cgattttgct caccatgcgg cttgcctctt gtggtttata gcccagcgcc accagcgcgg
    37321 caactgcttc ttgttcagca tcgtcggtcg ccgggctggc aggtgatgtg agtaccaggt
    37381 cggcggctgg cgtaaagaga tcgccatgca aacctttaaa tcggtctttc atttcaacaa
    37441 tcaagcgttc ggcggttttt ttgccaatac ccggcagttt caccagcgcc cccacttctt
    37501 cacgctcaac ggcattaacg aactgctgcg ctgacattcc ggagaggatc gccagcgcca
    37561 acttcgggcc gacgccgttg gttttgatca actctttgaa caatgtgcgc tcttgtttat
    37621 tgttaaaacc gtacagcagt tgcgcgtctt cacgcaccac aaagtgggtg aaaacgatcg
    37681 cttcctgacc cgcttcaggg agttcataaa aacaggtcat cggcatatgc acttcatagc
    37741 ctacgccgcc cacttcaatt aacaccagcg ggggttgttt ttcaatgatg atgcctctga
    37801 gtctgcctat cacatgacgc tcctgcgtaa tgaatcaaag ataatgctgt atgataaaaa
    37861 aatgctggat agatatccag cgaaggatga agaaaacttg cgaggtgtcc cgatgatctg
    37921 aaaaatggca gagtataatt tattcaacag gttatatt
//
LOCUS       DAPHDW010000031        37524 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.031, whole genome shotgun sequence.
ACCESSION   DAPHDW010000031 DAPHDW010000000
VERSION     DAPHDW010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 37524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37524
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.031"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            123..284
                     /locus_tag="RH057_001547"
     CDS             123..284
                     /locus_tag="RH057_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127939.1"
                     /translation="MEFSFSPKLFVVAVAAALPLMASAADTPSTATARKGFAGYDHPN
                     QYPQNAAIY"
     gene            complement(368..1603)
                     /locus_tag="RH057_001548"
     CDS             complement(368..1603)
                     /locus_tag="RH057_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV3127940.1"
                     /translation="MLQQVPTRAFHVMAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYVRHYIAASEPQNEVAFTWQGGEPTLLGLDFYRRAVKLQAKYGAGRKISN
                     SFQTNGVLLDDKWCAFLAENHFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAASDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLLVVNK"
     gene            complement(1750..1826)
                     /locus_tag="RH057_001549"
     tRNA            complement(1750..1826)
                     /locus_tag="RH057_001549"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1790..1792),aa:Pro,seq:tgg)
     gene            complement(1869..1955)
                     /locus_tag="RH057_001550"
     tRNA            complement(1869..1955)
                     /locus_tag="RH057_001550"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1919..1921),aa:Leu,seq:cag)
     gene            complement(1976..2051)
                     /locus_tag="RH057_001551"
     tRNA            complement(1976..2051)
                     /locus_tag="RH057_001551"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2016..2018),aa:His,seq:gtg)
     gene            complement(2110..2186)
                     /locus_tag="RH057_001552"
     tRNA            complement(2110..2186)
                     /locus_tag="RH057_001552"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2150..2152),aa:Arg,seq:ccg)
     gene            complement(2289..3674)
                     /locus_tag="RH057_001553"
     CDS             complement(2289..3674)
                     /locus_tag="RH057_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3127941.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWFPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3865..4605)
                     /gene="rffM"
                     /locus_tag="RH057_001554"
     CDS             complement(3865..4605)
                     /gene="rffM"
                     /locus_tag="RH057_001554"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HDV3127942.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISVVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4608..5960)
                     /gene="wzyE"
                     /locus_tag="RH057_001555"
     CDS             complement(4608..5960)
                     /gene="wzyE"
                     /locus_tag="RH057_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HDV3127943.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQALLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5957..7036)
                     /gene="rffT"
                     /locus_tag="RH057_001556"
                     /gene_synonym="wecF"
     CDS             complement(5957..7036)
                     /gene="rffT"
                     /locus_tag="RH057_001556"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HDV3127944.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNDHIAALCAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASVDKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(7033..8283)
                     /gene="wzxE"
                     /locus_tag="RH057_001557"
     CDS             complement(7033..8283)
                     /gene="wzxE"
                     /locus_tag="RH057_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HDV3127945.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEISQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8285..9415)
                     /gene="rffA"
                     /locus_tag="RH057_001558"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8285..9415)
                     /gene="rffA"
                     /locus_tag="RH057_001558"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HDV3127946.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVIST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDALAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGERFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9420..10094)
                     /gene="rffC"
                     /locus_tag="RH057_001559"
                     /gene_synonym="wecD"
     CDS             complement(9420..10094)
                     /gene="rffC"
                     /locus_tag="RH057_001559"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HDV3127947.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTPDVLAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASRRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(10072..10953)
                     /gene="rfbA"
                     /locus_tag="RH057_001560"
     CDS             complement(10072..10953)
                     /gene="rfbA"
                     /locus_tag="RH057_001560"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HDV3127948.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10972..12039)
                     /gene="rffG"
                     /locus_tag="RH057_001561"
     CDS             complement(10972..12039)
                     /gene="rffG"
                     /locus_tag="RH057_001561"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HDV3127949.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSEHFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELAPN
                     KPHGVAHYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(12039..13301)
                     /gene="wecC"
                     /locus_tag="RH057_001562"
     CDS             complement(12039..13301)
                     /gene="wecC"
                     /locus_tag="RH057_001562"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HDV3127950.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSTRASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13298..14428)
                     /gene="wecB"
                     /locus_tag="RH057_001563"
     CDS             complement(13298..14428)
                     /gene="wecB"
                     /locus_tag="RH057_001563"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HDV3127951.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGDLYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIDPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQTMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14484..15530)
                     /gene="wzzE"
                     /locus_tag="RH057_001564"
     CDS             complement(14484..15530)
                     /gene="wzzE"
                     /locus_tag="RH057_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HDV3127952.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSATAITDRPTVNMLGGYYSQQQFLRNLDARSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15542..16645)
                     /gene="wecA"
                     /locus_tag="RH057_001565"
     CDS             complement(15542..16645)
                     /gene="wecA"
                     /locus_tag="RH057_001565"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HDV3127953.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIIDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16885..18144)
                     /gene="rho"
                     /locus_tag="RH057_001566"
     CDS             complement(16885..18144)
                     /gene="rho"
                     /locus_tag="RH057_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HDV3127954.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18229..18330)
                     /gene="rhoL"
                     /locus_tag="RH057_001567"
     CDS             complement(18229..18330)
                     /gene="rhoL"
                     /locus_tag="RH057_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HDV3127955.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18471..18800)
                     /gene="trxA"
                     /locus_tag="RH057_001568"
     CDS             complement(18471..18800)
                     /gene="trxA"
                     /locus_tag="RH057_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HDV3127956.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18931..20196
                     /gene="rhlB"
                     /locus_tag="RH057_001569"
     CDS             18931..20196
                     /gene="rhlB"
                     /locus_tag="RH057_001569"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HDV3127957.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20330..21814
                     /gene="gppA"
                     /locus_tag="RH057_001570"
     CDS             20330..21814
                     /gene="gppA"
                     /locus_tag="RH057_001570"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HDV3127958.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENALSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQESQWQSYVHWPLEVH"
     gene            complement(21861..23882)
                     /gene="rep"
                     /locus_tag="RH057_001571"
     CDS             complement(21861..23882)
                     /gene="rep"
                     /locus_tag="RH057_001571"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HDV3127959.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEARGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPAQAAAEAKGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNHQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSPKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            24099..24548
                     /locus_tag="RH057_001572"
     CDS             24099..24548
                     /locus_tag="RH057_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HDV3127960.1"
                     /translation="MAINFSPKVGEILECNFGNYPVSQNGQISTAYYDGRIPPEMIKN
                     RLVVVLNGKINGNACIVVPLSTTRDHDKLNRGMHVEIASNVINDLQFFDQQIRWAKAD
                     LVQQVSRNRLNRARTYRGYLNQCLPHELVADIQRAVIKSINAISLIN"
     gene            24701..24982
                     /gene="ppiC"
                     /locus_tag="RH057_001573"
     CDS             24701..24982
                     /gene="ppiC"
                     /locus_tag="RH057_001573"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HDV3127961.1"
                     /translation="MAKTAAALHILVKEEKLALELLEQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(25028..26503)
                     /gene="ilvC"
                     /locus_tag="RH057_001574"
     CDS             complement(25028..26503)
                     /gene="ilvC"
                     /locus_tag="RH057_001574"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HDV3127962.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIARKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            26653..27546
                     /gene="ilvY"
                     /locus_tag="RH057_001575"
     CDS             26653..27546
                     /gene="ilvY"
                     /locus_tag="RH057_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HDV3127963.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPSLSGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMADQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILDRSDEKTPFELGVCA
                     QKKRLHEPLIEAFWRILPNHK"
     gene            complement(27598..29142)
                     /gene="ilvA"
                     /locus_tag="RH057_001576"
     CDS             complement(27598..29142)
                     /gene="ilvA"
                     /locus_tag="RH057_001576"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HDV3127964.1"
                     /translation="MADSQPLSGTPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(29145..30995)
                     /gene="ilvD"
                     /locus_tag="RH057_001577"
     CDS             complement(29145..30995)
                     /gene="ilvD"
                     /locus_tag="RH057_001577"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HDV3127965.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVANSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIEFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDMIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALRAYASLATSADKGAVRDKSKLGG"
     gene            complement(31060..31989)
                     /gene="ilvE"
                     /locus_tag="RH057_001578"
     CDS             complement(31060..31989)
                     /gene="ilvE"
                     /locus_tag="RH057_001578"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HDV3127966.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQVGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(32009..32272)
                     /gene="ilvM"
                     /locus_tag="RH057_001579"
     CDS             complement(32009..32272)
                     /gene="ilvM"
                     /locus_tag="RH057_001579"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HDV3127967.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(32269..33915)
                     /locus_tag="RH057_001580"
     CDS             complement(32269..33915)
                     /locus_tag="RH057_001580"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HDV3127968.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAAAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLATTKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQYC
                     AQLRDEHTWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDEFENVWPLVPPGASNSEMLEKLS"
     gene            complement(33918..33968)
                     /locus_tag="RH057_001581"
     CDS             complement(33918..33968)
                     /locus_tag="RH057_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3127969.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(34055..34153)
                     /gene="ilvL"
                     /locus_tag="RH057_001582"
     CDS             complement(34055..34153)
                     /gene="ilvL"
                     /locus_tag="RH057_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HDV3127970.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            34506..36026
                     /locus_tag="RH057_001583"
     CDS             34506..36026
                     /locus_tag="RH057_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HDV3127971.1"
                     /translation="MSLSIVHTRAALGVNAPPITVEVHISKGLPGLTMVGLPETTVKE
                     ARDRVRSAIINSGYEYPAKKITINLAPADLPKEGGRYDLPIAIALLAASEQLTANKLD
                     EYELVGELALTGALRGVPGAISSATEAIKSGRKIIVAKDNEDEVGLINGEGCLIADHL
                     RAVCAFLEGKHALERPKPTDAVSRALQHDLSDVVGQEQGKRGLEITAAGGHNLLLIGP
                     PGTGKTMLASRINGLLPDLSNEEALESAAILSLVNAESVQKQWRQRPFRSPHHSASLT
                     AMVGGGAIPGPGEISLAHNGVLFLDELPEFERRTLDALREPIESGQIHLSRTRAKITY
                     PARFQLVAAMNPSPTGHYQGNHNRCTPEQTLRYLNRLSGPFLDRFDLSLEIPLPPPGI
                     LSKTVVPGESSATVKQRVMAARERQFKRQNKLNAWLDSPEIRQFCKLESEDAMWLEGT
                     LIHLGLSIRAWQRLLKVARTIADIDQSDIITRQHLQEAVSYRAIDRLLIHLQKLLT"
     gene            complement(36051..36389)
                     /gene="maoP"
                     /locus_tag="RH057_001584"
     CDS             complement(36051..36389)
                     /gene="maoP"
                     /locus_tag="RH057_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HDV3127972.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            36508..37347
                     /gene="hdfR"
                     /locus_tag="RH057_001585"
     CDS             36508..37347
                     /gene="hdfR"
                     /locus_tag="RH057_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HDV3127973.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNTLIRDLLKINVLD
                     EVY"
     gene            complement(37443..37518)
                     /locus_tag="RH057_001586"
     tRNA            complement(37443..37518)
                     /locus_tag="RH057_001586"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37483..37485),aa:Trp,seq:cca)
BASE COUNT         9169 a        10213 c         9452 g         8690 t
ORIGIN      
        1 ataaataaat aatttatttt tgtcactatt atttatgtaa tcatcctgtc aaggagaggg
       61 atctcaatta tcaatgctta attacgtcat cattttgatg acatgaaacc ggagcatttt
      121 gcatggaatt ttcgttttca cccaaacttt ttgttgttgc tgtcgccgcc gctcttcctc
      181 tcatggccag cgcagcagat accccgtcaa ctgccaccgc acgcaaaggc tttgccggat
      241 acgatcaccc aaaccagtat ccacagaacg ctgcaattta ttgaatttgc acgatcatgt
      301 aggccggata aggcgtttac gccgcatccg gcaatcaacc gcaggcggcc acaaaatggc
      361 cgctttttta cttattcacc accagcaaat gtgcctgcat aatgtcactg gccgggcgac
      421 cgtgcgccag caaatcagcc attgctttaa gatatggcgg tagatggcgg aaataacgct
      481 gatacccggc acacaaataa ttcagccccg gtttgccgct ggcatcgagc atgaagcggt
      541 gtttcgggca gcctccccag cacgctttta acacgttaca actgcgacac tgcgccggta
      601 attgcttaaa tttatcttca ccaaacacct gctgttgcgg ggaatcgatc atttctgcaa
      661 ttgtttgctg atgcatattc cccagccgat attgcggata aacatagtga tcgcaggcgt
      721 aaacgtcgcc gttgtgctca acaatcaccg agcgcccaca ggttggctga tgatggcaaa
      781 ccgcacccgg cgcaccgaca aaattggcaa acgcccattc gatattcatc acgaaaatct
      841 tgccgacgtc gcgtttgatc cagtggtcga atatcgccac cagaaattca ccaaattcat
      901 cggggcgcac cgaccattcc gtcagttccc cctgaatatc accaggcgca tgtagtttca
      961 gtccatcgct ggcagctgtt tcatcagcca ggcgctcgac caccggaata aactggatga
     1021 attcgactcc cgcatcgcac aaaaaatcat aaacctgcaa cggttgctgc gcgctggtgc
     1081 ggttgacgca gaccagcacg ttatagtcga catgatgttt ttgcagcagc gtcagggcac
     1141 gcatcaccag cttatgcgtt ggtctgccac ctttggtcac gcgatattga ttgtggatct
     1201 cagccggacc gtccagcgat aacccaacaa gaaaatgatt ttctgccaga aatgcacacc
     1261 atttatcatc gagcagcacg ccgttagtct ggaagctgtt acttatcttc ctgccagcac
     1321 cgtatttcgc ctgtaacttt acggcacggc ggtaaaaatc cagcccgagt aacgttggtt
     1381 cgccgccctg ccaggtaaaa gccacttcgt tttgcggttc gctggcagcg atatagtgac
     1441 ggacatacgc ttccagcgtg tcatcgtcca tatgcgtgac tggcttttcg cggtaaaggg
     1501 attgtttttc gagataaaaa cagtagtcac agttcagatt acaatcggaa ccactcggtt
     1561 tcgccatcac atgaaaagca cgcgttggaa cctgttgcag catggtcact ccttgcctga
     1621 agtcatcctt caatacgtta agggcgaaat cataccgtgg ggcaatgagt tattacgggc
     1681 gtttattgcg aagggagggt gacgaaatgc acagaaaaca aaaaaccccg ccgaagcggg
     1741 gttcaaaatt ggtcggcgag agaggattcg aacctccgac ccactggtcc caaaccagtt
     1801 gcgctaccaa gctgcgctac tcgccgaaat actgcttttt gaatttttag ttcaattctt
     1861 taaagtcgtg gtgcgagggg ggggacttga acccccacgt ccgtaaggac actaacacct
     1921 gaagctagcg cgtctaccaa ttccgccacc ttcgcattgt cacaacttct aataatgggg
     1981 tggctaatgg gattcgaacc cacgacaact ggaatcacaa tccagggctc taccaactga
     2041 gctatagcca ccactacaaa tcttgttacg cggtattact accaccgcag ctcaagcgcc
     2101 gggactaaat ggcgcgcccg acaggattcg aacctgagac ctctgcctcc ggagggcagc
     2161 gctctatcca gctgagctac gggcgcttag cgccgttgcg ggggtggata atacggactt
     2221 cccacccctc tgtctagtcc ctttttaaat aaattgcgcg tttggttacg gtttgtgcgt
     2281 tttgctgctt attcctccag tttatgcgct ttcccgtggc gattaaggcc aaaaacttta
     2341 taaatcgccg tcaccgccag cataaagatg atgccaacaa acagcgacat acgcgtatct
     2401 tcattaaagt acatgccgat caaaacgcaa atcaggaatg ccattgttac gtagttggcc
     2461 cacgggaaca ggattgagcg gaacggatgg ctggcaatcg ccgctttatg tgcacgccga
     2521 aaacgcagct ggcttatcaa tatcacaaac catggcacca tccccggaag cacgctggca
     2581 ctgtagacgt agacaaacac acgctgcgga ttgggaatga tgtagttcag gcatgagcca
     2641 attagcagaa tagcaataga tactgccaca cccgcaacgg gtacgccgtg acgggaaact
     2701 ttcgccatcg ccgccggtaa ctgacggttt ttcgccagtg cgtagagcat acgtccgcaa
     2761 ctgtacatgc cgctgttaca gccagagagc gcagccgtca gcaccacaaa gttgataatg
     2821 cccgccgctg cggtaatacc gattttggca aaagtcagta cgaacgggct gccgttgctg
     2881 cctatttcat tccacgggaa gatggtgacg ataacgaaaa tcgcgcctac gtagaaaatc
     2941 aggatccgcc acagcacctt gcctacggca ctgcgcagcg tcacctgcgg attcttcgct
     3001 tcaccggcag taatgccaat cagctccacg ccctggtagg acgccaccac aatacacagc
     3061 gcggtcagga accctttcca gccacccgca aagaaaccgc catgctctgt gagattgcta
     3121 aaaccaatcg actgcccgcc attgccaaag ccaaagaaaa tcacgcccag gccaatgaca
     3181 atcatcacga taatcgtggt gactttgatc atcgcgaacc agaactcgat ttcgccgtac
     3241 aaccgcaccg ccgccagatt cgccaacgcc accagcgcca ctgcgatcaa tgcgggtatc
     3301 cactgcgcca tctccgggaa ccagaactgg acataaacgc caatggcggt gatttctgag
     3361 atccccaccg ccatccacat aaaccaataa gaccaggcgg tgagataacc aaagaacggg
     3421 ctcatataac gatgcgcata aacggcgaac gaaccggtaa ccggttcgag gaacaacatt
     3481 tcgcccattg aacgcatgat gaaaaagacg aatagcccgg cgatgatata ggccaacaac
     3541 acggatggcc cggcccattt caaggtactg gcggccccca taaacaggcc gacgccaatg
     3601 gtgcccccca gggcgatgag ttcgatatgt cgagcttcca gcccacgctg tagctctggt
     3661 ttgttatctg ccataaatcc tcgtgttgtg tttgcatgct ttccggtgtt accggttatc
     3721 gttatgggta catcgagtgt tgcgaatgtt ttcgtaattc aggagaaatg gcaaataaag
     3781 cattaaaaat ttgaatgctt tgtgtaataa aaaagcagac aggcgacgga gtgaccactc
     3841 cgtcgcttta caaagagagg aaaatcagag gttgccggtg tagtgccagc gtaaataacg
     3901 cagcaaacga agctgacgct taatgcggct cggctgcgaa agcaggcggt agagccactc
     3961 cagtcccagc gtttgccaga ttttcggtgc gcgttttacg tgaccggtga aaacatcgta
     4021 agtcccgcca acgcccatat acagcgcatc tggatgcacc agacggcagt cgcgcatgaa
     4081 gatctcctgc tttggcgatc ccatcgcaac ggtgacgatt tgcgctccgc tggcatgaat
     4141 gcgttcaaac agcgcctgac gctgctcggg tttaaaataa ccatcctgac tgccaacgat
     4201 attcacattc cattggttgc gcagtttagc ttcagtttgc gccagcactt caggtttacc
     4261 gcccacaaga aataccggcg tcccttcttt gcctgcgcgc gccatcagct cttcccagag
     4321 atcggcaccg gcaacgcggg aaacctgcgc ctgcgggtac tttttacgta ctgaacgtac
     4381 aacgctgatg ccatccgcat atttaaattc ggcagcgtta attaactccc tgacctcggc
     4441 gttatcttca atggtcagca ttttttcggc attaatggca accagcgttc cctgcttaag
     4501 ctgcccgtca gcaaacagat aatcgagggc gtgctgcata tcacgccaac caatcaactg
     4561 taagccacgc agcgtatagg tcggtgccgt ggtgttgtta ttcattgtta tccttcaacc
     4621 tgcgtccgga gcgatgattt tgtacgttta tgaatgagtc cggcgctttc aaaaagccag
     4681 tacaacagtt ttgcgatcat cagacatgcg ccgaagacca cgataaaaaa gactacgcgt
     4741 gagacaaacg aatccagccc ttcacgtgcc agcacgatca tattgaagat cgccccaaag
     4801 cagaaactgt gcaatatcgc agccttatag cgattagtct cgcggttgcc cagctcatac
     4861 agccagtcga accatttgat gatcagtcca accacaatcg ccccgagtgg gatgaacaac
     4921 gcgccgccca tcaccaccag tgagcctata agcgtaggcg agatcgccag tccggagtgg
     4981 ttattcagca cttcccaggt aaagtagttg gctgagttca gcaccatact cgggcgaccc
     5041 ggccacagcc aggaagggat aaagacatag aaatcgcgga caattggagc caggccctgg
     5101 aagtcgatgt tgtcgtagtt ctgcaacagc aacgccagat tctcccatgg cgagaaggtg
     5161 tcgcgagtga gatagagaaa cgtatagaac gcttcatcgc cgctcacatt cattccatag
     5221 cgttttagcg ccagccagaa catgcccaca atccccagca cgcccgccgc cgccagcatc
     5281 cacaacgaaa tccagccgcg aataatgcca ataaacagga agatagcgaa tgcgatgatg
     5341 atattggcgc gagtgccgcc gacaatcata taagtcagca agccaaaggc gaccgtgctg
     5401 acgaggaaaa acagccacgc tttgctatcc tggcgcagaa agtagaccac cagcatcgcc
     5461 gggatgaaaa agtaaaagaa gcgttttaac gccacgccgg agacttcact ggagaagatc
     5521 tgactgtagg agttaagccg gaacagcaaa aagccgttgt gcatgaagaa gatgccgacg
     5581 cttaccagcg cgatgcccat caggatcacc cacgtaagat tggtctccac gcggttcatg
     5641 gtaaacagcg gacggcgcgg tacatcagca acgcgtttgc gtaggcgggt tttgtaggtg
     5701 acatagtaaa ccgcgtagaa gcagcccgca gaaagcaacg cctgcaacaa gatttctggc
     5761 ggcgcgacac caacatcaaa gcgaaatacc agcacgctgg tcagcgggaa gccgaagaaa
     5821 aaggtgagca aaaacagcaa tgaaaagaag acattgaagt taaagcgcac acggcgaaac
     5881 tcaaaccagg tcagcgtggc aataaacagc gtgcagagca gccagacaac aaacaggcca
     5941 ctgaattgca gcagactcat gcgacctccc cggcggcaat cgccaacgcc cgctgccagc
     6001 cttgtagata gttagggcta aagaaggcaa tggtgttttt atccaccgac gccaactggc
     6061 gctgcgcttc acgcacaata tcctcgttga gatcgtcggt agtaaacagc accggcaaat
     6121 gctgttccgt catatcctgc cagaacggat tttcccggtt aagcacacaa ggaatgcccg
     6181 cctgaatcag taagcaaagc gtaccaatgc cctgctggcg ggcaaaaata aagtaaccaa
     6241 gatcgcactg acgaagtagc gccagatagg cgtcaaattc cagtttttcg ctcaaaattt
     6301 gcagattttc ttcgctgaat aactccagcc ccgcctgacg aacttcctca atgtacgctt
     6361 cgttattagg cggatatccc atcggcacca ccacttttac cgtatcgcca aattgctgat
     6421 gaacggcgca caaggcagca atatggtcat tgctgcggtc accggagttc cccaccagaa
     6481 tggtcatttt cccttcacgt tgccgatcgt tcgccatcgt attgagagaa gggtccatcc
     6541 gcgtcgggaa gtacagcagt tcgccccgca cctttggatg cgttttggca aaaaagctca
     6601 aatcgccgcg ggtggcaaat acacagccga ctcgcttttg cgccaggcga cgtagtgggt
     6661 aaaaaagctt atatctcaag ccactggaaa gctcatacag gtctgccccc cagatatgcc
     6721 agaaaaactg gctgggctta atgccaccac tcagcagagc cagccacagt gtgggattga
     6781 actgaccgtg gaagaaaaaa cgctgctgac ggttagcttt tgctttcgcg atgaccgctt
     6841 ccgccagcga ttttttccca gggaaaaatt gcacagaaag cgccggacag ctatcactta
     6901 agccgtcgtc cttgccaaca accataaact cgcgcgcgtg ctcgctcgtc gcagccagcg
     6961 cgtcattgaa aaaccgcaaa acggttcggt tatggtgagg gatatccgat cccagtacgt
     7021 gaatcagtac agtcatgccc gcctacgcca gagtaaaaac acgccacaac aaagagaaaa
     7081 atagacgata taagttgcca tatatgcctg cgccgcgccc agtgcaccat gcgcagggat
     7141 tagccagtgg gcaaatacca tcaataaagt gaactggctg atttccgcca gaatataaaa
     7201 ccgcaatgac gctttggcga tcaccagata accaaagaca taagcgccca cttttaacac
     7261 atcacccact aactgccagg caaagagatc gcgcatagcg gtaaatttat tcgacaacag
     7321 cagccagata gcaaaatcac gcagcagcca gacggtaaaa ctcgccgccg ccactgccgg
     7381 taagacgaat ttcagcgatt taaccacttc ccgggtgata tcgcgctttt ccgttagccg
     7441 cgacaacgtg ggcagcaaat atacgctgaa cgatgccgta ataaattgca ggtaggcatc
     7501 ggaaatactg ctcacccctt gccagatccc cacttcatcc cagctatact gcgccgcgag
     7561 cagtttacgc atcatgatgt aagcaacagg caaggtcacc gacgtaatca acgccatgag
     7621 cgtaaatttg ctcaactgcc ctgccagacc gttatcccag ctgggtttca gatagcttaa
     7681 cgggatgaca ccacgtttga tcaacatgat ggcggcagga attaccacca gcgcgggaat
     7741 cagcgccaga cccagcaacg ccccttcata accgcccaaa cggtaactga cgtagtacgc
     7801 gagaacgcca atcaagctgc cgacaatcag cgataacgca ttacctgcgg catcgcgaaa
     7861 gcctttcatc agcgccagta acaggttgcc ccaggcgatc cccatttgca ccagcgccac
     7921 taaacgcacc agcccctgat agtcggtatt accaaacaat ccctggctga ttggcgcagc
     7981 tgccagcaca aaaaccagcg ccatcagcgt agagaaacca agtaccatcg ctgatgaagt
     8041 gccgaccacg cggcgcagct gttgcggatt atcatggtac tgggcaacgt atttggttac
     8101 accgttaaag atgccagctc cggcaagcac gccgagcacg gtaatcaact ggcggaaatt
     8161 tgccgccagc ccaagccccg ccggaccaaa tgacacagcc agcaacttac cgaccagtaa
     8221 cccggcacca atcttgacca gtgtactggc cgccgtccac aaggacgctt ttgccaacga
     8281 catatcagga gaagtagttc aacaaagtcg caattaccgt acgctgattg acgggcgaca
     8341 ggttgtagaa cagcggcagg cgcagcaggc gctcgctctc tttggtggtg tagcgatctt
     8401 caccgtggaa ctcaccaaag cgttcccccg cagggcaacc gtgcagcgga atgtaatgga
     8461 acaccgccat gatttccgct tctttcagaa agttaatcaa cgcgctccgg tcatcaatat
     8521 cccgcagttt aatgtagaac atatgcgcgt tctgcacgca gccatcggga atcgacggca
     8581 gctcgatacg cccggctttc gccagaggcg ctaacgcatc gtagtagttt tgccacagcg
     8641 ccagacgttg ctggttgata cgatccgctg cttccagttg cgcccacagg tacgcagctt
     8701 gcagatcgga catcaaatag ctggagccaa tatcgcgcca ggtatattta tcgacctgac
     8761 cacggaagaa ctggctgcgg ttagtgccct tttcacggat gatctcggct cgttcgatta
     8821 acgctttatc gttaatcagc gtcgcgccgc cttcaccacc cgccgtatag tttttggttt
     8881 catggaagct aaagcagcca atatgaccaa tggttcccag tgcacgccct ttgtaagtgg
     8941 atatcacgcc ctgagcggca tcttctacca caaacagatt atgctttttc gccaacgcca
     9001 taatggtgtc catttcgcag gccacacccg cgtaatggac cggcacgata acgcgcgttt
     9061 tgtcggtgat cgccgcttca atcagcgttt cgtcgatgtt catggtgtcc gggcgaacat
     9121 ccacaaaaac gatttttgcg ccacgcagca caaaggcatt ggcggtggag acaaaggtgt
     9181 agctcggcat gatcacttca tcgccaggct ggatatcgag cagcagcgcc gccatctcca
     9241 gcgaagcggt gcaggacggc gtcagtaaca ctttggcgct gccaaaacgt tgctccagcc
     9301 actgctggca gcgacgggta aaaccgccat cgccacacag tttgccgcta cccattgccg
     9361 actgcatata gtcgagttcg gttcccacca ccggcggtgc gttaaatgga atcatgtgat
     9421 cacctgtata accagtacgc ggtgctttct acatttgcac cactttgtat gtatcgttta
     9481 agcgcggcgg tgttgcccat ttgggtcgcc acccgcaaag ttgttttacc gcgagcatac
     9541 gcccagttta gcgccgtttg catcagctca gcacctgcac cgcgtccagc cagcaggcca
     9601 attcgcgcat ctgtcgcatt gagttcccgt aaagagacat agccgcgaat atcgccggac
     9661 gccgcacgta aaatcagaca ttgatgatca aaggtgccgc gcacggcatt ttcaatccac
     9721 tgtgcataaa agcgtctgct ggcgtcaggc gcataccacg gcgcacgaaa acggctttgc
     9781 gcaaatgcgg cgctggctaa ctgacgtaat gcgggaatat cggtctcttg tgccactaca
     9841 gcaccactat cactgacatt gttcacgggt agtgccaaat caacttcacc ttctaccagg
     9901 gagaatccca gctgttgcag ggcatccagt tcacccgtat ttgatgccgc aattttggcc
     9961 tgcacccgtg accacggcgc taacacgtct ggcgtcagga gcggtgcttc agacgtaatg
    10021 cgcacgatgg cgctgttaac accaaagaag gcgttttccc aggttagtgg ctcaatactg
    10081 gcgcggacgg gcacgaagta actccagcag atattggccg tagccagttt tcgctaatga
    10141 actggcagca cgcttcacac cctcgtcatc gagccagccg ttacgccagg cgatctcttc
    10201 caggcaggca atcttaaagc cctggcgttt ttctactgtc tgcacaaagg tactggcttc
    10261 aatcaggctg tcgtgagtgc cagtgtccag ccaggcaaat ccgcgcccga gcagttcaac
    10321 ggtcaggttg cccgcctcga ggtacatctg gttgatggag gtaatctcca gttcaccacg
    10381 ctccgacggc ttcacctgct ttgcgtactc cacgacttta ctgtcgtaga aataaagccc
    10441 ggtcaccgcc cagtttgact tcggctgttt tggcttttct tccagcgaga tagcgcggaa
    10501 attgtcgtca aactccacca cgccaaagcg ttccgggtcc atcacctgat agccaaatac
    10561 cgtcgcccct tccgtgcgcg ccgcaacatg acgcagcttc ggactgaagc cctgaccgaa
    10621 gaagatgtta tcgcccagca ccagacaaga aggttcaccg ttgaggaagg tttcaccgat
    10681 gataaaggcc tgcgccagac cgtccgggct gggctgttcg gcgtattcca gctggatacc
    10741 gaactcacta ccatcgccca gcaggcgctg gaaataacct ttatcttccg gcgtagtgat
    10801 gatgagaatt tcgcggatac cggccaacat cagcaccgac agcggatagt aaatcattgg
    10861 cttatcgtaa atcggcaaca gttgcttcga tacgccgcgc gtaatcggat gcaatcgggt
    10921 gccggaaccg cccgccagga taataccttt catgtttgcc tccgctgaaa attagccttt
    10981 cagacctaaa cgctcgccct gatagctgcc gtcctgcacc tgcttccacc agctttcatt
    11041 agccagatac cactgcaccg ttttacgcat tccactttca aaggtttcct gtggcagcca
    11101 accaagttca cgggcaattt tcgaagcatc aatggcataa cgcagatcat gccccggacg
    11161 gtcagcgaca aaggtgatca agtcacgata gtgcgccacg ccgtgcggct tgttcggagc
    11221 cagttcttcc agcagctcgc aaatggtttc cacaacatcg agattcttac gctcgttgtg
    11281 accaccaata ttataggttt caccgacttt cccggtggtc gccacgcaat acagcgcgcg
    11341 ggcgtgatct tccacataca gccagtcacg gatttgctgc ccgttgccat ataccggcag
    11401 cggtttaccc gccagcgcgt tgaggatcat cagcgggatc agtttttccg gaaagtggta
    11461 aggaccgtag ttattcgagc agttggtgat cagcgtcggc agaccgtagg tccgcagcca
    11521 ggcgcgcacc agatggtcgc tactggcttt tgacgcggaa taagggctgc tcggcgcata
    11581 cggcgtggtt tcggtgaaga aatcatccgt cgagtgcagg tcaccatata cttcgtcggt
    11641 ggagatatga tgaaaacgga acgctgattt tttatcttcc gtcagcgtat tccagtaagc
    11701 ccgcgccgct tcaagcaatg tataagtccc gacaatgttg gtttcaataa acgctgccgg
    11761 gccgtcaata gaacggtcaa catggctttc ggctgccaga tgcatgacac agtctggctg
    11821 atgctcagtg aatacgcgtg ccagttctgc ccgatcgcag atatcaactt tctcaaaggc
    11881 aaagtgctcg ctttgcgcga ccggtgccag cgacatcagg tttccggcgt aggtcagctt
    11941 atcgaccact accaccgcat cgctcgtttc gttgatgata taacgcacca gcgccgagcc
    12001 aataaacccg gcaccacctg ttatcagaat ttttctcatc agcgccagac tcctttggca
    12061 tcgacgacat actgctgatg gacattgtcg ccattgataa ctttgaactg actatgatcg
    12121 accagcatca ccagcacgtc tgccgttgcc agcgcctcgt caagctgcgc cagagtacaa
    12181 agcccggtca gttttttcgg caactggtgg atgttaggct caacaaccag cgtttcgccg
    12241 ctgtgccact gggcgatcag ttcagcgatt tccatcgccg ggctttcgcg caggtcatca
    12301 atattcggtt taaacgccag accaaagcag gcgattttca gttcactggc gcgtttatcg
    12361 gtagcagcaa ggcagtcggc aaccgccgct ttcacctgat cgataaccca gaacggtttg
    12421 tgatcgttaa cttcgcgcgc ggtacggata agccgcgcct gctgggggtt ctgtgccacg
    12481 ataaaccacg gatcaacagc aatgcagtga ccgcccacgc cagggccagg ctgaagaata
    12541 ttaacgcgag ggtgacgatt cgccaggcga atcagttccc agacgttaat cccctgatcg
    12601 gcacaaatca gcgacaattc attagcaaaa gcgatattca catcgcggaa gctgttttcg
    12661 gtgagcttac acatttccgc cgtccgcgag ttagtgacga cacactcacc ttcgaggaaa
    12721 attttgtaca gttcgctggc gcgggtcgaa caaaccggcg tcataccacc aatcacgcga
    12781 tcgtttttaa tcagctcgac cataacctgg cccggtaaca cgcgttccgg gcagtaagca
    12841 atgttgacgt ccgcctgctc gcccacctgc tgtgggaaag tgagatccgg acgcatctct
    12901 gctaaccatt ctgccatctt ctcggttgac cccaccggcg aggtggattc aaggatcacc
    12961 agcgcgcctt ttttcagcac tggcgcaatg gagcgagcag ccgattcaac gtaggtcata
    13021 tctggctcat gatcgccttt aaacggcgtg ggtacagcaa tcagccaggc atccgcttca
    13081 actggcgtcg tgctcgctcg taaaaaaccg ccttctacgg cagtttttac tacgctcgcc
    13141 aaatcaggct cgacgatatg gatttcgcca cgattgatgg tatcaaccgc gtgttggttg
    13201 atatcgacac caatcacctg tttttgccgt gaggcaaacg ctgctgctgt aggcagcccg
    13261 atgtaaccca gtccgataac agaaatggtc gcaaaactca tagtgatatc cgattatttt
    13321 ttaacgcttc cagaatgcga gagcatgcct gaccatcacc atacgggtta tgggcgcggc
    13381 tcatagtttg atattcgttt tcgtctttta aaagacgcgt cacttcctcg acaattcgct
    13441 gcttatccgt gcctaccaga cgcaccgtac ccgccgtcac cgcttccgga cgctcagtgg
    13501 tatcgcgcat caccagcacc ggtttcccca gcgaaggcgc ttcttcctga atgccgcctg
    13561 agtcggtcaa aatcagccag gcgtggttca tcagccagac aaacggtaaa tactcctggg
    13621 gatcgatcag aatgacattt ttcacatgcc ccagaatgcg attgaccggc tctctgacgt
    13681 tcgggttgag atgcaccgga tagacaatct ggatgtcctg gtgcgtggtg gcgatgtctg
    13741 ccagcgcgtg gcagatttct tcaaagccac gaccgaaact ttcacgcctg tgaccggtca
    13801 ccagaatcat ctttttatcg gggtcgataa acgggtaatt tgccgctagt tctgaacgca
    13861 gcgtgtcgct gctcatcacc tgatcacgca cccataacag tgcatcaatg actgtattac
    13921 cagtaatgaa gattcggcta tccgcaacgt tttcacgcag caagttttgc cgggaagttt
    13981 cggttggaga gaagtgatac atcgccagat gcccggtcaa tgtacggtta gcctcttccg
    14041 gccacggcga atagagatcg cccgtgcgta aaccagcctc aacgtgacca acaggaatac
    14101 gctgataaaa cgccgccagg ctggttgcca gcgtcgttgt tgtgtcgccg tgaaccagca
    14161 cgacgtctgg tttgaactcg gcaagaatag gttttagccc ttccagaatc cgacaggtta
    14221 tctctgtcag gccctgtcct ggctgcatta tgttgagatc gtagtcaggt acaatggaaa
    14281 agagtttcag cacctgatcg agcatctccc gatgctgcgc agtgacgcaa actttagcct
    14341 caaaaaaagg atcttttgcc aacgcatgca ccaacggagc catcttgatg gcttccgggc
    14401 gcgtaccaaa tacagtcagt actttcacat cgattctctt cgaataagcg gcgagcgcct
    14461 ttgcgctcgc cgcagcagtg ttgctatttc gagcaacggc gggttaatgc gacaccagcc
    14521 ccgatcagcc ccccgacaat gccccacata atcatcagga aggcacgacg tgggctatcg
    14581 cgttttaccg gttcttccgg cgtacgcaaa tagcgatagg tctgaaaacg cggatccaga
    14641 gttggaccaa cattcagggt gtttaacatg gcccgattct gatcatagtc gagatcaaag
    14701 gccggaccga cggcctgtaa attttccagt cgagcctgga gcattggacg cccaagcagg
    14761 aacatttctg aatcaggtaa ttcctcggca ggcacatctg tcgcactgcg cgaaatatta
    14821 tgctgctcag caattttcag cgcctgctca atgctgttca tccggcggtc gtagatggct
    14881 ttcgccacct cttcctgacg cttcacctga gctttcatct ggatggtgcg tgccgcccat
    14941 gcgcctttca gctcatcatt cagatggctg gctgcacgct ggctggcaaa agcaacatac
    15001 tgacgtaaca ggttattagc gtcaggcgcg gtttcggcaa taagcttcac gctgtcattg
    15061 accgcgcgag taaagtctcc ggggataaac tggatgttgt taatcatttc atccagcaac
    15121 gccgcatcgg ctttgctgtt gcccaccatc cgctgcttgt aatagtcggt ttgcagccag
    15181 aactctctgc gggtatccca cgaggccagt tgcataacaa actctttata ggcttcgtcc
    15241 atgaccgatg gttggtcggc agaagccatg tttgaacggg catccaggtt acgcaaaaat
    15301 tgctgctgcg agtaatatcc ccccagcata ttcaccgttg gacgatcggt aatcgccgtc
    15361 gcgctccact cttgtcgagc aaaaaaagta tacgccagcg cgattaacgc aaacgccagc
    15421 cccatgccaa taatccatag cttcccagcc cacaaggtac gaaacaaccc acgaatatcc
    15481 agttcatttt cagcgtcttc ggccggtttc ccaggcattg gttgtgtcat cacatcctca
    15541 tttatttggt taaattgggg ctgccaccac gatttctacg cagtctgcgt tttacgcgct
    15601 taataaagcg agcaactttc caggcacgct taatgcaata tccatagagg aagaatgcta
    15661 gcaaaaagag caccagcatg acccactccg ggacaaaatg agaatattct gccagcacgc
    15721 caatggaagc gagcagtgct gcggcaaggg taatcagcac aaacgcctga cgggaagtaa
    15781 acccggcacg catgatcaaa tggtgaatat gctgacggtc aggagagaat gggctcatgc
    15841 ctttacgcag gcgacggtac ataatcgcca ccatatccat tagcggaatg gcgattatcc
    15901 acaaagcggt aaccgggctg atgggatggg ttttgccctg ggtcgtttcg agcaggatcc
    15961 agataacggt aaaaccaatc agcgtactgc ccgcatcacc cataaagact ttgtagcggc
    16021 gacccaggat accaaggtta agcatgatgt atggcaggat ggcggcgatc atcgcaaagc
    16081 accagattgc gaggctggtt tgcccgtcga accacaaaat cataccgatt gctgcaaacg
    16141 agacgcagga caacccgccc agcaagccat caatgccatc aaccatgttg aacgcattaa
    16201 tggccgccca gacggcaaat agcgtcagga agtaaccaaa cggtccgagc accatctccc
    16261 aggagccaaa gatataaccc aggctactga gataaagttt gccgaacacc atcataacaa
    16321 tgccaacagc ggcctgtatg gtggcacgga tttttacgct gatatcaaaa cggtcatcca
    16381 gcgcgccaat gaaaacaagc acaccggcac aagcgagata gagagatgca tgcggaatat
    16441 agtaatcgat aattccgaac gtgaagcaaa tccctgcgta aaccgaaatc cccccaacga
    16501 gaggtatcaa tccctggtga cgtttgcgga agtttggttt atccactaaa ccgacttttt
    16561 ttgccacttt acgggcaaaa aacagaaaca gtgtcgtgaa taaaaaaata ctgatgagat
    16621 cagtactcac tgtcagtaaa ttcacaatgc atgctctcag agaaaattat tagcagaagt
    16681 ataaccacga agacctttat tcagaaggga aagtccgttc cgaacccacc agtcctgtgc
    16741 aaaaattaac catttgatat gcaaattgca taacatgcca gcaaattcca ggaattttcc
    16801 taacatcgcc agcgcggcat taagattaca gatataaaag caaaacgcca cgtaaacacg
    16861 tggcgttttt ggcataagac aaatttatga gcgtttcatc atttcgaaga aatcgtcatt
    16921 ggtcttagtc atggccagtt tattaatgag gaattccatt gcatcgattt cgcccatcgg
    16981 gtgaatgatt ttgcgcagga tccacatttt ctgcagttct tcctgagtcg tgagcagctc
    17041 ttctttacgg gtaccggaac ggttgtagtc gatagccggg aagacgcgtt tttcagcgat
    17101 cttacgagag aggtgcagtt ccatgttgcc tgtaccttta aactcttcgt aaataacttc
    17161 gtccatttta gaaccggtat cgataagcgc cgtcgcgata atggtcaggc tgccgccctc
    17221 ttccacgtta cgcgccgcac cgaagaagcg tttcggacga tgaagggcat tggcgtccac
    17281 accaccggtc aacactttac ctgacgccgg aacaacagtg ttgtaagcgc gcgcaagacg
    17341 agtgatggag tcgagcagaa tgataacgtc tttcttgtgc tcaaccaggc gtttggcctt
    17401 ctcgatcacc atttccgcaa cctgaacgtg gcgagatgcg ggttcgtcaa aggtagaagc
    17461 aacaacttca ccttttacca gacgctgcat ctcggttact tcttccggac gttcgtcgat
    17521 cagcagaacc atcagcacac aatccgggtg gttgtaagca atgctctgag caatgttctg
    17581 cagcagcata gttttaccgg ctttcggcgg tgccacaatc agaccacgct gaccacgacc
    17641 gataggtgat gccagatcca gtacgcgagc ggttaaatct tcagtagaac cgttaccacg
    17701 ttccatacgc agacgagagt ttgcgtgcag cggggttaag ttctcaaaga ggattttgtt
    17761 gcgggcgttt tcaggtttgt cgaagttaac ttcgttaact ttcagcagcg caaaatagcg
    17821 ttcaccttct ttcggcgggc gaatcttacc agagatggta tcaccagtgc ggaggttgaa
    17881 acggcggatt tggctagggg aaacgtagat gtcatcagga ccggcgaggt aggagctgtc
    17941 tgcggaacgg aggaaaccaa atccatcctg caatatctcc agtacgccat caccaaagat
    18001 atcttcgcca ctctttgcgt gctgcttcag gatggcaaaa ataatgtcct gcttacgcat
    18061 acgagccagg ttttccagcc ccatattttc gccgagagtg atcagctcag aaaccggcgt
    18121 attctttaat tcggtaagat tcatagtggt gtgagttctt aaacttgggg taaatctcga
    18181 acttaatgtt gtgaatggta tggcaggatc atccatgcct gtttacggtc atcgactcat
    18241 gtcttttcgc tgcctggtca caggagagta cgcagaactg aaacgacaag acggattgag
    18301 tgacgagcca gaaatctgtt cacttcgcat ttaagataaa acgggaagcg ttggaaatta
    18361 caagattcaa acttaataag gtatgtttaa tacgaagtca acactaagtt agcatgactc
    18421 acgccgggcg tccagttttt agcgacgggg cacccgaaca tgaaattccc ttacgccagg
    18481 ttagcgtcga ggaactcttt caactgacct ttagacagtg cacccacttt ggttgccgcc
    18541 acttcaccgt ttttgaacag cagcagagtc gggataccac ggatgccata tttcggcgca
    18601 gtgccagggt tttgatcgat gttcagtttt gcaacggtca gtttgccctg atattcgtca
    18661 gcgatttcat ccagaatcgg ggcgatcatt ttgcacggac cgcaccactc tgcccagaaa
    18721 tcgacgagga tcgccccgtc cgctttgagt acatccgtgt caaaactgtc gtcagtcagg
    18781 tgaataattt tatcgctcat atataactcc acaggaataa gcctggcgtg ttggtttcgt
    18841 tgttggtgta acattaacca actaaaggtt gactttattt caccggatac gctttcgtaa
    18901 agcaatagta agctgatatt ctaccacact atgagcaaaa cacatttaac agaacagaag
    18961 ttttccgact tcgccctgca tccgaaggtt gtagaagccc ttgaaaaaaa agggtttcat
    19021 aactgtacgc ccattcaggc actggccctt ccgctgacgc tggcgggtcg tgacgtagcc
    19081 gggcaggcgc aaaccggtac cgggaaaacg atggcgtttc ttacgtcaac gtttcattat
    19141 cttctctctc atcctgcgat tgccgatcgc aaggtgaatc aaccacgtgc cttaattatg
    19201 gcaccgacgc gtgaacttgc cgtgcagatc catgccgacg cagaaccgct ggcggaagct
    19261 accggcctga agctgggtct ggcttacggt ggtgatggct acgacaaaca gctgaaagtg
    19321 ctggaaagcg gcgttgacat tctgattggc actacggggc gtttaattga ctacgccaag
    19381 cagaaccaca ttaacctcgg tgccattcag gtggtggtac tggacgaagc cgatcgcatg
    19441 tacgatctgg gctttattaa agatatccgc tggctgttcc gccgtatgcc gcctgcaaac
    19501 cagcgcctca acatgctgtt ctccgccacg ctttcgtacc gggtacgtga actggcgttc
    19561 gagcagatga acaatgccga atatattgaa gtggaaccgg aacagaaaac gggccaccgt
    19621 attaaagaag agcttttcta cccttctaac gaagaaaaaa tgcgtttgct gcaaacgctg
    19681 atcgaagaag agtggccaga cagagcgatt attttcgcca ataccaaaca ccgttgtgaa
    19741 gagatctggg gccacctggc ggcagatggt catcgtgtcg gtttgttgac aggcgatgtc
    19801 gcgcagaaaa aacgtctgcg tattcttgat gaatttaccc gtggcgatct ggatattctg
    19861 gttgccaccg acgttgccgc gcgtggtttg catattccgg cagtgacgca cgtctttaac
    19921 tacgatttac ccgatgactg tgaagattac gttcaccgta ttggtcgtac aggtcgcgca
    19981 ggcgcaagcg gtcactctat tagcctggcg tgtgaagagt atgcattgaa tttgcctgct
    20041 attgagacct atattggtca ctcaattccg gtaagcaaat acaatccgga cgcattgatg
    20101 accgatctgc caaaaccgct gcgcctcacg cgcccacgta caggcaatgg tccgcgtcgt
    20161 actggcgctc cgcgtaatcg tcgtcgttca ggttaaataa aatgtcatac tcaaaatcat
    20221 tcaaactgta tcaagatggc aagagaatga gtcccgatga gcgtactgta gtaagtgatt
    20281 cgggtgaaag aatgcagcca acacagagac agattgaagg atgaagagta tgggttccac
    20341 ctcgtcgctg tatgcagcca ttgatctcgg ttcgaatagt tttcatatgc tggttgtgcg
    20401 cgaggtggct ggaagcatcc agacgctgac gcgaattaaa cgcaaagtgc gtctggctgc
    20461 tggcctgaat agcgaaaatg ccctctctaa tgaagcaatg gagcgcggtt ggcaatgtct
    20521 gcgcctgttt gctgaacgtc tgcaagatat tcctccctcg caaattcgcg ttgtcgctac
    20581 ggcgacatta cgacttgccg tcaatgcggg tgattttatt gccaaagcgc aggaaatcct
    20641 cggttgtccg gtacaggtga tcagcggtga agaggaagca cgtctgattt atcagggcgt
    20701 tgctcacacc actggtggtg ccgatcagcg cctggtggtg gatataggcg gagccagtac
    20761 tgaactggta accggcacgg gtgcacaaac cacctcgttg ttcagcctgt cgatgggctg
    20821 cgtcacctgg ctggaacgct attttgccga tcgtaatctg gggcaggaaa attttgatgc
    20881 tgcagaaaaa gcggcacgcg aagtgttacg tccggttgcc gatgaattac ggtatcacgg
    20941 ctggaaagtg tgcgttggcg cttccggcac cgtgcaggcg ttacaggaaa tcatgatggc
    21001 acagggaatg gatgaacgca ttaccctgga aaagttgcag caactgaaac agcgcgccat
    21061 tcattgcggt cggctggaag agctggagat tgacgggctg acgctggaac gtgcgttagt
    21121 gttcccgagt ggtctggcga tcctgatcgc catttttacc gaactgaata ttcagtgtat
    21181 gaccctggcg ggcggtgcgc tgcgtgaagg cctggtctac ggcatgttgc atctggccgt
    21241 cgagcaggat attcgcagcc gtacgctgcg taatattcag cgccgcttta tgatcgatat
    21301 tgatcaggcg cagcgcgtag ccaaagttgc agctaacttc ttcgatcagg tggaaaatga
    21361 atggcatctt gaagcaataa gccgcgattt gctcatcagc gcttgtcagc ttcatgaaat
    21421 cggcctgagc gttgacttca aacaagcgcc gcaacacgct gcttatctgg tgcgtaacct
    21481 ggatcttccc ggttttaccc ccgcacagaa gaaactgctg gcgacgctac tgctcaacca
    21541 gactaacccg gtcgatctct catcgctgca tcagcaaaat gccgtaccac cgcgcgtcgc
    21601 agaacaactc tgccgtttac tacgcctggc tatcattttt gccagccgtc gccgtgacga
    21661 tctcgtgcca gagatgacat tacaggctaa ccatgaactg ttgaccttga cgcttccgca
    21721 aggttggcta acccaacatc cgctgggtaa agagattatt gctcaggaaa gccagtggca
    21781 gagctatgtc cactggccgc tggaagtgca ttaatgagta agtgccggat gcgatgctga
    21841 cgcatctttt ccggccttga ttatttccct cgttttgccg ccatcatcgc tttcaaattc
    21901 gccagatggc tttgcccttt ctgcatccgt tcttcggcgc tgaccacttt gcgctcctgt
    21961 tcccaaatca gatcatcctg cggcagctcc agcaaaaagc ggctcggctc cgggcgcacc
    22021 agttcgccat actgacggcg ttctttacac agcgtaaagg tcaattcctt ctgggcgcgg
    22081 gtaatgccga cataggccag ccgccgctcc tcatcgatat tatcttcatc gatgctgctc
    22141 tggtgcggca aaaacccttc ttccataccg accatgtaga cataaggaaa ctccagccct
    22201 ttcgacgcgt ggagagtcat cagttgcacc tgatccagct cttcttcact ctcaccacgc
    22261 tccatcatgt cgcgcaaagt aaagcgcgtc accacctggg tgagcgtcat cggctcatcc
    22321 agttcgctgc cttccagcat ctccgtcatc cagctaaaca gttggttgac gttcttcatg
    22381 cgcatttcgg cggctttcgg gctgggcgat gtttcgtaca gccaggattc ataatccatg
    22441 ccgtggatca gatcgcgcac cgcagcaatc ggctcccgct ccgccaggcg ctggatttct
    22501 gccaaccagt gagtgaagcg ggtcaatgct tcataaccac gtccgctaag ggtctgactc
    22561 aggcccatat caaagctggc ggtaaacatg cttttattgc gcgtcatcgc ccactcaccc
    22621 agctttttca gcgtcgccgg gccaatctct cgcttcggcg tgttaacgat acgcagaaat
    22681 gcgctgtcat cgtccgggtt agtcagcaca cgcagataag ccagcaaatc cttgatttca
    22741 ggacgagaga aaaacgacgt accaccagaa attttgtacg ggatgcggtt ttgcatcagg
    22801 aatttttcaa atacccgcga ctgatggttg ccgcgataaa gaatggcgta atctttgtac
    22861 tgcgttttat tgacgaagtg atgcgcaatc agttcaccgg tgacgcgctc ggcttcatgt
    22921 tcctcgttat tcgcgcttaa aacttttagc tctgtgccat aacccagttc ggagaacaga
    22981 cgcttttcaa agacgtgcgg attattggcg atcaggatgt tcgccgcttt caaaatacgt
    23041 ccggaagagc ggtagttttg ctcaagctta atcaccttca gcgccggaaa atcctggctc
    23101 aacagcacca ggttttgcgg acgtgcgccg cgccaggagt agatcgactg gtcgtcatcg
    23161 ccaactacgg taaagcgcgc gcggctgccc accagcagtt tcaccagttc atactggctg
    23221 gtgttggtgt cctgatactc atccaccagc aggtagcgaa ttttgttctg ccagcgctcg
    23281 cggacttctt cattgcgttg cagcagcaac gtcggcaata aaatcagatc atcgaagtcg
    23341 agaacgttac aggctttcag gtgtgcatca tacagcccat aacaatgggc aaaaatacga
    23401 tcccgctcgc cctttgcctc tgccgccgcc tgcgccggag ttttgagatc gttcttccag
    23461 ttggagatgg tcgaaattag ctgttgcagg agaactttgt catcttcaat cagcccctca
    23521 gtcagctctt tgagcaacgc cagctgatcg gtatcgtcaa acaatgagaa gttcgctttc
    23581 atcccaagcg ccgcatactc acgcttgatg atatccagcc ccagcgtgtg gaaggtggag
    23641 atcatcagcc ctcgcgcctc tttgcgaccc agcgtctgcc cgactcgctc tttcatctcg
    23701 cgcgccgcct tattggtaaa ggtcaccgcc gcgatatgtc gtgcctgata accgcaaccg
    23761 cggatcagat gggcgatttt attggtgata acacgagttt taccggaacc cgcgcccgcc
    23821 agcaccaggc aggggccggt aacgaattcg acagcttgtt gttggccggg gtttagacgc
    23881 ataggtgtat tgctcaatct tcgaacgggg ggaggattgt agcagaatcg catttgaatg
    23941 tgaccgagtg aaaaccgcta accggatggc tgcggatgtt taaatcagag agggaaggcc
    24001 gaaaccggag ttcatcatga atccattaga cttagaaaat atcgggtcgt aacgttgtgt
    24061 tattactttt tcagagttta ctaaggagag gtttactcgt ggcgattaat ttcagcccca
    24121 aggttggtga aatactggaa tgcaactttg gtaactatcc tgttagccag aatggacaga
    24181 tcagtacggc gtattacgat ggccgcattc cacctgagat gataaaaaat aggttggttg
    24241 tcgtgctcaa cggaaaaatt aatggcaacg catgtatcgt cgttccatta tcaaccactc
    24301 gcgaccatga caagttaaat cgtggaatgc atgttgaaat agccagtaat gttatcaatg
    24361 acctgcaatt ctttgatcag cagatacgtt gggctaaagc cgacctggta caacaagtca
    24421 gtcgaaaccg attaaacaga gcaaggactt atcgggggta tcttaatcaa tgtttgccac
    24481 atgagttggt tgcagacata cagagggcag tcatcaagtc aatcaatgcc atatcgctca
    24541 taaattgaca ataaaatcac catccccgat aatgactctg tagcccatag aggccctaag
    24601 ttttctcccc ttgagggacc tgcgaagccc ggattttccg ggcttttgcc atatatgtcc
    24661 ccccctccct gactgacaat ttttaattca aggcacgatc atggcaaaaa cagcagcagc
    24721 actgcatatc cttgtaaaag aagagaaact ggctctggaa cttctcgagc agattaagaa
    24781 cggtgccgat ttcggcaagc tggcgaagaa acactccatt tgcccatcag gcaaacgcgg
    24841 cggtgattta ggtgaattcc gccagggtca gatggttccg gcgttcgata aagttgtgtt
    24901 ctcttgtccg gtactggagc caaccggccc gctgcacacc cagttcggtt accacatcat
    24961 taaggtgctg taccgtaact aatagcaagg ccttctccgg gagaaggcct tgaactctga
    25021 atcgctatta acccgcaaca gcaatacgtt tcatatccgt catatagccg cgcagtttct
    25081 tacctacttg ctcaatcgca tggctgcgaa tcgcttcatt tacatcacgc agctgcgcat
    25141 tatctaccgc accttccgga atagctttac ccaggtcgcc cggttgcagt tctgccataa
    25201 acggtttcag cagcggcaca caagcgtaag agaacagata gttaccgtac tcggcagtat
    25261 cagatataac cacgttcatt tcatacagac gcttacgggc gatagtgttg gcaatcagcg
    25321 gcagttcgtg cagtgattca taatatgcag actcttcaat gatgccggaa tcgaccatgg
    25381 tttcgaacgc cagttcaacg cccgctttca ccatcgcgat catcagtacg cctttatcga
    25441 agtactcctg ctcgccgatt ttgccttcat actgcggcgc ggtttcgaag gcggttttgc
    25501 cggtctcttc acgccaggtc agcagtttct tatcatcgtt ggcccagtcc gccatcatac
    25561 cggaggagaa ttcgccggag atgatgtcgt ccatatgttt ctggaacagg ggtgccatga
    25621 tctctttcag ctgttcagac agcgcgtaag cacgcagttt cgccgggtta gagagacggt
    25681 ccatcatcag ggtgatgccg ccctgcttca gcgcttcggt gatggtttcc caaccgaact
    25741 gaattagttt ttctgcgtat gccggatcgg tgccctcttc caccagcttg tcgaagcaca
    25801 gcagcgagcc agcctgcaac ataccgcaca ggatggtttg ctcacccatc aggtcagatt
    25861 tcacttccgc aacgaaggac gattccagca cgcccgcacg gtgaccaccg gttgctgccg
    25921 cccatgcttt agcgatcgcc ataccttcgc ctttcggatc gttttccggg tgaacggcaa
    25981 tcagcgtcgg tacgccgaat ccacgtttgt actcttcacg cacttcggtg ccagggcatt
    26041 tcggcgcaac catgactacg gtgatatctt tacggatctg ctcgcccact tcgacgatgt
    26101 tgaaaccgtg cgagtaaccc agcgccgcgc cgtctttcat cagtggctgt acggtgcgca
    26161 ctacatcaga gtgctgcttg tccggcgtca ggttaaccac cagatccgcc tgcgggatca
    26221 gttcttcgta agtaccaact ttaaaaccgt tttcggtcgc tttacgccag gacgcgcgct
    26281 tctcggcaat cgcttcttta cgcagagcgt aggagatatc gagaccagaa tcacgcatgt
    26341 tcaggccctg gttcagcccc tgtgcgccac agccgacgat aaccactttt ttaccctgaa
    26401 ggtagctcgc gccgtcggcg aattcgtcgc gccccataaa gcgacatttg cccagctgtg
    26461 ccagctgctg gcgcaggttc agtgtattga agtagttagc catggtgatt cctcgtgatg
    26521 ttgtgcttct tattgttcgg ttcgcgtttg cgagattgaa ttcactatat gacaggaaat
    26581 ttattgcgga aattgatata ttcacaacgt cacattgcaa tttctgcaac gtcaacattg
    26641 agggctgcgc cattggattt acgcgatctg aaaaccttcc tgcatctggc ggaaagccgc
    26701 cattttggcc gcagcgcgcg ggcgatgcac gttagcccat ccacgctctc acggcagatt
    26761 cagcgcctgg aagaagatct tggtcagccg ctatttgtgc gcgataaccg cacggtaacg
    26821 ctgaccgaag cgggcgaaga gttacgcgtt ttcgcccagc aaacattgtt gcagtatcag
    26881 caactgcgcc acaccatcga tcagcaaggg ccgtcgctct ctggcgagtt acatattttc
    26941 tgctcggtga ccgccgctta cagccatcta ccgccgattc tcgaccgctt ccgcgcggaa
    27001 cacccgtcgg tggagatcaa actcaccact ggtgatgcgg cagatgcaat ggaaaaagtg
    27061 gtcacgggag aagcggatct ggcgattgcc gggaaaccgg agacgctacc cggcgctgtg
    27121 gcgttttcga tgctggagaa tctggcagta gtgctgattg cccccgcgct gccctgcccg
    27181 gtgcgtaatc aggtatctgt cgaaaagccc gactggtcaa cggtgccgtt tattatggcc
    27241 gatcaggggc cggtacgccg ccgcattgaa ctgtggtttc gacgcaataa aatcagcaac
    27301 ccgatgattt acgccacggt tggcgggcat gaagcgatgg tatcgatggt ggcgctcggc
    27361 tgtggtgtgg cgctgttgcc ggaagtggtg ctggaaaaca gcccggagcc tgtacgcaac
    27421 cgggtaatga ttttagatcg cagcgatgag aaaacgccgt ttgaactggg cgtgtgtgca
    27481 caaaaaaagc ggctgcatga gccgctgatt gaggcgttct ggaggatttt gccgaaccac
    27541 aaatgacgtt gtcgcgcggg taggcctgat aagcgaagcg ctatcaggca tttttcccta
    27601 acccgccaaa aagaacctga acgccggatt attggtttcg tcgtggcaat cgtagcccag
    27661 ctcatttagc cgggtttcga aatccggttc atggtcgccg agttcgaacg ccgccagtac
    27721 gcgcccgtag tcggtgccat ggctgcgata gtggaacaaa gaaatgttcc agtgcgtacc
    27781 cagcgtgttg aggaagcgca gcagcgcgcc cggtgattcc gggaattcga agctgtagag
    27841 gcgttcctgc aacggatgtg acggacgccc gccgaccata tagcgcacat gcaacttcgc
    27901 catttcgtcg tcggagagat ccaccacgct gtagccgccg tcgttgagca tctgcaaaat
    27961 ttctttgcgc tcttcgaggc cgcggctcag gcgcacgccg acaaagatgc aggcgttttt
    28021 ggcatcggca aaacggtagt tgaactcggt gaccgatcgc ccgccaagca gttggcagaa
    28081 tttgaggaag ctgccttttt cttccggaat ggtcaccgcc aacaacgctt cacgctgttc
    28141 gcccagttcg cagcgttccg agacgtagcg caggccgtgg aagttcacgt tggcaccgga
    28201 aagaatatgc gccagccgtt cgccgcgaat gttgtgctgg gcgatatatt ttttcattcc
    28261 cgccagcgcc agcgcgccag agggttccgc caccgcgcgc acatcttcga ataaatcctt
    28321 catcgccgca cagatcgcat cactatcgac ggtgatgatg tcgtcgagat actcctggca
    28381 taaacggaag gtttcgtcac cgatgcgttt taccgctacg ccttcagcaa atagccctac
    28441 gcgcggcaga tcaaccggat gacccgcatc cagcgctgct ttcaggcagg cggagtcttc
    28501 cgcttctacg gcgatcactt tgatttgtgg catcagttgt ttgatcagca ccgcaacgcc
    28561 cgccgccaga ccgccgccgc cgactggcac aaatacgcgg tcgagatggg cgtcctgctg
    28621 gagcagttcc agcgccagcg tgccttgccc ggcaatcacc atcggatggt cgaacggcgg
    28681 cacccaggtg aacccctgct gctgtgacag ttcgatcgct ttggctttcg cttcatcaaa
    28741 gttcgcgccg tggagcagca cttcgccgcc gaagccgcgc accgcatcaa ctttgatatc
    28801 ggcggtggcg gttggcatga cgatcagggc cttcacgcct aaccgtgcgg aagaaaacgc
    28861 gacgccctgc gcgtggttac ccgcagaagc ggtaatcacg ccgtgcgctt tttgttcttc
    28921 cgtcaggccc gccatcatgg cgtatgcgcc acgaagctta aagctgtgca ctggctggcg
    28981 atcttcgcgc ttcaccagaa tcacgttatc gagacgcgac gacagttttt ccattttctg
    29041 tagcggcgtg acctgcgccg cttcgtagac cggcgcgcgc agcaccgctc ttaaatattc
    29101 ggcaccttcc ggggtaccgg acaggggttg cgagtcagcc attattaacc ccccagtttc
    29161 gatttatcgc gcaccgcacc tttgtcggcg ctggttgcca ggctggcgta ggcgcgcaac
    29221 gcaaaggaga cctgacgttc gcgatttttt ggcgtccagg ctttgtcgcc acgagcttcc
    29281 tgcgcttcac gacgcgccgc cagttcggca tcgcttacct gtaactgaat gccacggttc
    29341 ggaatgtcga tagcaatcat gtcgccgtct tcaatcagac caatgctgcc gccgcttgcc
    29401 gcttccggcg agacgtggcc gatggaaaga ccagaggtgc cgccggagaa gcgtccgtcg
    29461 gtgatcagcg cgcaggcttt gccgaggccc atcgatttca ggaagctggt tgggtagagc
    29521 atttcctgca tgccggggcc gcctttcggg ccttcgtaac ggatcaccac cacatcgcca
    29581 gcgacaactt tgccgccgag aatcgcttct accgcgtcgt cctggctttc gtacactttc
    29641 gccggaccgg tgaatttgag gatgctgtca tcgacgcccg cggttttaac gatgcagccg
    29701 ttttctgcga aattaccgta gagcaccgca aggccaccgt ctttgctgta ggcgtgttcc
    29761 agcgagcgga tgcagccgtt tgcgcgatcg tcatcgagag aatcccaacg gcaatcctgc
    29821 gagaatgcct gtgtggtacg aatgcccgcc gggccagcgc ggaacatatt ttttaccgcg
    29881 tcatcctggg tcagcataac gtcgtattgt tccagcgttt gcggcaacgt caggccaagt
    29941 acgtttttca catcacggtt cagtaacccc gcacgatcca actcgccgag aataccgata
    30001 acaccaccag cacgatgaac atcttccata tggtacttct gcgtactcgg cgcgacttta
    30061 cacagctgcg gtaccttgcg ggagagctta tcgatatcac tcatggtgaa ttcgatttcc
    30121 gcttcctgcg ccgccgccag caggtgaagt accgtgttgg tagagccgcc catcgcgata
    30181 tccagcgtca tggcattttc aaacgccgcc ttactggcga tattacgcgg cagtgcactt
    30241 tcgtcgtttt gctcgtagta acgtttggtc aattcaacaa tgcgcttacc agcattgagg
    30301 aacagttgct tgcgatcggc gtgggtcgcc agcagcgagc cgttgcccgg ctgcgacagg
    30361 cccagtgctt cggtcaggca attcatagag ttagcggtaa acatcccgga gcaagatccg
    30421 caggttggac aggcggaacg ttcaacctga tcgctctggg agtcagaaac tttcgggtct
    30481 gcgccctgga tcatcgcatc aactagatcg agcttaatga tctgatcgga cagtttggtt
    30541 ttcccggctt ccatcgggcc gccggaaaca aagatcaccg gaatattcag gcgcagggaa
    30601 gccatcagca tccccggggt gattttgtcg cagttagaga tgcagaccat ggcgtcggcg
    30661 cagtgagcgt tgaccatata ctcaacggaa tcagcgatca gttcgcgaga tggcagtgaa
    30721 taaagcatcc ccccgtggcc catggcaatc ccatcatcca ccgcaatggt gttgaactct
    30781 ttggcaacgc cgccagccgc ttcaatttgt tctgcgacca gtttaccgag atcgcgcaga
    30841 tggacgtgac ccggtacaaa ttgggtgaac gagttcgcaa ccgcgataat cggcttgcca
    30901 aaatcggcgt cggtcattcc ggtggcgcgc cacagcgcac gagcacccgc catattacga
    30961 ccatgagtgg tggtggcgga acggtactta ggcatacttt atttactccc agtgtctgtc
    31021 tcgtaaatgg gacggtgcgt gccgtcccat tttttgtatt tattgattaa cttgatctaa
    31081 ccagccccat ttatcttcgg tttcgccagt gaagaggccg aagaaggctt gctgaatgcg
    31141 tttggtaacc gggccgcaac ggccttcacc aacctgaata ccatctacac tgcgcactgg
    31201 cgtgatttct gccgccgtac cggacataaa cacttcatcc gccagataca gggattcgcg
    31261 cgacagcacc tgctcacgca cttcaattcc caactctttc gccagtttga tgatggcgtc
    31321 acgggtaata cccggcagcg cggaggaggt gaacggtggg gtgaacagca caccatcttt
    31381 cacttcaaac aggttttcgc ctgcgccttc agagatataa ccgttcacat ccagcgcgat
    31441 accttcctga taaccgtggc ggcgcgcttc gctacccacc agcagggaag agaggtagtt
    31501 accaccggct tttgccgccg tcgggatggt gtttggtgct gcgcggttcc aggaggaaac
    31561 catcgcatcg atcccctgct ccagcgcttc tgcgcccaga tacgctcccc acgggaaagc
    31621 ggcgataatc acatcggttg agtatcccgc tggcgggtta acgcccatac caacatcacc
    31681 gacgaagatc agcggacgga tataggcgct ggtgagattg tttttgcgga tcacgtcacg
    31741 acaagcttcc atcagctcat caatgctctg cgaaaccggg aagcgataga ttttggcgga
    31801 gtcatgcaga cgctgcatat gctcacgatg gcggaataca accggtcctt tgtgcgagtc
    31861 gtagcaacgg atgccttcaa aaaccgaggt gccatagtgc agcgcgtgcg acatcacatg
    31921 caccttcgcg tcttcccagc gaaccatctc cccattgaac caaatgtaat cagctttctt
    31981 cgtggtcatt tttatattcc ttttgcgctc aggcgcggat ttgttgtgat gtggttgtgc
    32041 tctggcagat ggcaacgtgt gcgacgtcca ccagtttatt taactgacta aacagtaagt
    32101 cgaccgaccg tgggctggca acggtcaatt cgatatttat attttgtgca tcgctggcgg
    32161 cggccatatt cattgaacag acgtggaaac cacgatgacg caccacgcgt aacacacgtt
    32221 ctaaggtttc cggattgaag cgagccgata cattgacctg atgttgcatc atgataattt
    32281 ctccaacatt tctgaattac tggcaccagg cggcaccagc ggccagacgt tctcaaattc
    32341 gtcgattgag acatgaagca ggtatggccc atcactgttc agcatggtgt cgagtgccgc
    32401 ttcaacctgg tctttacggg tgatgtgttg gccagggatg ccgaaggcgc tggctaacat
    32461 gagaaaatcg gggttatcgg taagggtggt ttcgctatat cgttcctgga aaaacagttg
    32521 ctgccattgt cgaaccatcc ctaaccgttg gttatcgagt aagacgattt tcaacggtaa
    32581 ctgcttacgt tttacggtgc ccagctcttg tacattcatc atgaaagagc cgtcaccgga
    32641 gatacagacg acggtatcgt tcggtcgcgc gacttgcgcg ccaaccgccg ccggtaaacc
    32701 gaaacccatg gtgcctaagc cgctggaggt gatgaaattt tccgggcgag tatgggcgat
    32761 gtgctgcgca gcccacatct ggtgctgccc cacatctgtg gtcacgacgc aatccgcagg
    32821 tttacgatcc gacagttgtt ttaacaacaa tggcgcgtag atagcgtcac cgggatggtc
    32881 gtaacgccag gtatgttcat cacgcagctg cgcgcagtat tgctgccagt cattgatatt
    32941 taacggctgc tgtaatgctg gtaacagagc atttaaatca ccttgtaatg ccacatgtgc
    33001 ctgacgcagc ttgttcattt ctgccgggtc gatatccata tggataacac tggcgtgtgg
    33061 tgcgaaggtg ttcagtttgc cggtcacccg gtcatcaaaa cgtgcaccca cggcgatcag
    33121 caagtcgcac tcctgcaccg cgaagttcgc cgctttggtg ccatgcattc ccagcatgcc
    33181 caggtagtac ggataatctg cttcaactgc gcccagccct ttcagcgtgc aggtggcagg
    33241 catttttgtg gtagcgagaa attctcgtaa agcaggaact gcctgcgcca tacccacgcc
    33301 accaccaacg tacagcatcg gtttttgcgc ttttgccagc atctggcgcg cttgctcaac
    33361 ttcggcatgg gggaaagtca cttcgttttc aacggtggtg aaccacggtt ccagatcgcc
    33421 gctggctaac tggatatctt ttgggatatc gaccagaacc ggaccaggac gacctgagct
    33481 ggcaacgtcg aatgctgcag ccatgatgcg cggcaactct tccagcgact gcaccagaaa
    33541 gctgtgcttg gtacaggcta acgacaatcc caggacatcc acttcctgaa atgcgtcggt
    33601 gccgataaac ggtgcggaca cttgaccggt gatggcaacg acagggatgg aatctaacag
    33661 tgcgtccgca agcccggtta tcaggttggt tgcgcccgga ccagacgtgg cgatacatac
    33721 gccagtttta ccggtagcac gggcataacc gatagccgcc attgccgcac cctgctcatg
    33781 tcggcacagc aagtgctcca cgccgccgtc atacaatgca tcgtaaaccg gcataattgc
    33841 gccacccgga taaccgaaaa cggtgttcac accctgtgcc cgcaacgcat gtaccaccca
    33901 ctgtgcgcca ttcatagtta gttccccgtc ctgaatcttg agaaacagaa ttttgtgctg
    33961 ttattcattg tctgctcctc ggttatgttt ttaaggtcaa aaaaaacccc cggacctttc
    34021 ggtgcggggg tcttagttcg ttaaggcttg atttctaagc ctttcctcgt ccaagtgcag
    34081 ccccgcacgg tgggataata atcaccacca cgctaatcac gaccaggcta atcactcgta
    34141 gaagggctgt cattttgtct tttcttgcat cttgttcgaa ggaatgccta aagagttacc
    34201 ataggttttg taaatagtac aagatatttt ttggccgatt tttatgatgc gtttaataat
    34261 ttcaatataa attgttgttt tttaaaaata aaatagtgag tgaaaaaaat tcaatttcag
    34321 tatgaatgtt gttcccttcc tcgtagttca tcctttgtcc gctgcatttt tgcgagcatc
    34381 attccaccgc cagaacaact caaattcact tgcacaaata aacaggaatc agccactaaa
    34441 attcacgaat tagcgggcta cagcagcgat atcttatgca cataatggtg actcaaggag
    34501 ggcttatgtc actgtcaatt gttcataccc gcgcagccct gggagtaaat gcgcccccga
    34561 tcactgttga ggtacatatc agtaaaggtc tacccggctt aacgatggtg ggcttaccag
    34621 aaacaacggt aaaagaagct cgcgatcgcg tgcgcagcgc cattatcaat agcggatatg
    34681 aatatccggc caaaaaaatc accatcaacc tggctccagc cgatctgcca aaagaagggg
    34741 gacgatatga tttacctatc gccattgcgt tgctggcggc ctcagaacag cttacagcca
    34801 ataagttaga tgaatatgaa ttagtcggag aactggcgct tacaggcgct ctgcgtggcg
    34861 ttcccggtgc aatctccagt gcaactgaag ctattaagtc gggcagaaaa attatcgtcg
    34921 cgaaagataa cgaagatgaa gtggggctaa ttaacggtga aggatgcctg atagccgatc
    34981 atctgcgggc tgtctgtgcg tttctggaag gtaagcacgc tctcgaacgc ccgaaaccaa
    35041 ctgatgcagt atcccgggcg ctacaacatg atctcagtga tgttgtcggt caggagcaag
    35101 gaaagcgagg actggaaatt accgccgctg gcgggcacaa ccttttactg attgggccgc
    35161 cgggaacagg taaaacaatg ctcgccagcc gtattaatgg cctgttgcca gatttaagca
    35221 atgaagaggc actggagagc gctgcgatat taagtctggt aaatgctgaa tcagtacaaa
    35281 aacaatggcg gcagcgcccg ttccgctcac cgcatcacag tgcgtcgtta actgcgatgg
    35341 tgggcggtgg tgcaattcca gggccaggtg aaatttcgtt ggcgcataac ggcgtgcttt
    35401 ttcttgatga gctacctgaa tttgaacggc gtacactgga tgccttgcga gagccgattg
    35461 aatccgggca gatccatctt tcacgcacac gagccaaaat aacctatcca gcccgtttcc
    35521 agcttgttgc ggcgatgaat cccagcccta ccggacatta tcagggaaac cataaccgct
    35581 gcacgccaga acagacatta cgttatctca accggctctc ggggcccttt ctcgaccgct
    35641 tcgatctctc actggagatc ccattaccac cccccggcat tttgagtaaa acggtagtgc
    35701 cgggagaaag cagcgccacc gttaaacaac gcgtaatggc cgccagagag cgccaattta
    35761 agcggcagaa taaactgaat gcctggctgg atagtccgga aatacgccaa ttctgcaaac
    35821 ttgagagcga agatgcgatg tggttggaag gaacactgat ccatctgggg ttatcgattc
    35881 gtgcctggca gcggttattg aaagttgcac gaaccattgc tgatattgat cagtctgaca
    35941 ttatcacacg tcagcatttg caggaggcag ttagctatcg tgcgattgac cgtttgctca
    36001 tccatctgca aaaactactg acataaaaaa agggcatttc gcccttttta ttaatcatca
    36061 gaatcggtgt agtcttcagc accttcaacc tgcggtttac cgccggaaag ggtgtgaaaa
    36121 cgttttggac gcttaatacg cgtcatatac ttggaccaca cgcgttctgc ttctgtcact
    36181 ggctcacgtt cgccacggca tactgctacg aagagtttct cttcctcggt aaccggctcg
    36241 cgtttgccaa gatccaactc attgaaggca taaccatgac gctcaagcag ttgtgcctct
    36301 ttgatggtga aatcaccatg acgagagaat ccacgtggat aatgtttatt gtcgaaatat
    36361 cgattagtcg tcgtaaagct ttccgccatc ctgcacgctc ctaattcttt gaccgagcta
    36421 gttatggcgc ggagtattag ttacgcttga cagagtgtaa aacaaaacat ttaaatcata
    36481 acgacaaata attttgcgga gagcactgtg gatacggaat tgttaaaaac tttcctggaa
    36541 gttagccgaa cgcgtcactt tggtcgagcg gctgaatcgc tctatctgac ccagtcagca
    36601 gtgagctttc gaatcagaca actggaaaat caactgggtg tgaacctttt cacccgccac
    36661 agaaacaata tccgtttaac cgctgccggt gaaaaactac tgccttatgc agaaacgctc
    36721 atgagcacct ggcaggccgc ccgcaaggag gtggcgcata cctcacgaca taacgagttt
    36781 tctatcggtg ccagcgcctc attgtgggaa tgtatgctta atcagtggct gggacgtttg
    36841 tatcaaaatc aggatgccca tacaggctta cagttcgaag cgcgaattgc ccaacggcag
    36901 tctctggtaa aacagctgca tgaacgccag cttgatcttc ttattaccac tgaagcgccc
    36961 aaaatggacg aatttagtag tcagttgctg ggatatttca ctttagcgct ttataccagt
    37021 gccccttcaa aactaaaggg agatcttaat tatctgcgac ttgagtgggg gccagatttt
    37081 caacagcatg aggcaggttt gatcggtgct gacgaagtgc ccattctgac aaccagttct
    37141 gctgaactgg cacagcaaca gattgcgatg cttaatggtt gcacctggct acccgtcagc
    37201 tgggcgcgta aaaaaggcgg cctgcatacc gttgtcgata gcacaacact ttcacggccg
    37261 ctttatgcca tatggctgca aaatagcgat aaaaatacgt tgattcgcga tcttttgaaa
    37321 attaacgtgc tggatgaagt gtattaatat gaatggctgg caaggatgcc ggtagaagga
    37381 tttacttcgg agagggttat ttcagataaa aaaaatcctt agctttcgct aaggatgatt
    37441 tctggcaggg gcggagagac tcgaactccc aacacccggt tttggagacc ggtgctctac
    37501 caattgaact acgcccctaa ttag
//
LOCUS       DAPHDW010000032        36550 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.032, whole genome shotgun sequence.
ACCESSION   DAPHDW010000032 DAPHDW010000000
VERSION     DAPHDW010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 36550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36550
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.032"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            241..1458
                     /gene="alaA"
                     /locus_tag="RH057_001587"
     CDS             241..1458
                     /gene="alaA"
                     /locus_tag="RH057_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HDV3127974.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            1542..2141
                     /gene="yfbR"
                     /locus_tag="RH057_001588"
     CDS             1542..2141
                     /gene="yfbR"
                     /locus_tag="RH057_001588"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV3127975.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNSQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(2200..4032)
                     /locus_tag="RH057_001589"
     CDS             complement(2200..4032)
                     /locus_tag="RH057_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HDV3127976.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTGVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTDEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(4119..4769)
                     /gene="hxpA"
                     /locus_tag="RH057_001590"
     CDS             complement(4119..4769)
                     /gene="hxpA"
                     /locus_tag="RH057_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HDV3127977.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEHIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(4780..5274)
                     /locus_tag="RH057_001591"
     CDS             complement(4780..5274)
                     /locus_tag="RH057_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HDV3127978.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMEMYHALHVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(5357..5812)
                     /locus_tag="RH057_001592"
     CDS             complement(5357..5812)
                     /locus_tag="RH057_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HDV3127979.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGQLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            6150..7352
                     /gene="ackA"
                     /locus_tag="RH057_001593"
     CDS             6150..7352
                     /gene="ackA"
                     /locus_tag="RH057_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HDV3127980.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVVFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            7427..9571
                     /gene="pta"
                     /locus_tag="RH057_001594"
     CDS             7427..9571
                     /gene="pta"
                     /locus_tag="RH057_001594"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HDV3127981.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPAF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            9761..11281
                     /gene="yfcC"
                     /locus_tag="RH057_001595"
     CDS             9761..11281
                     /gene="yfcC"
                     /locus_tag="RH057_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HDV3127982.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGDASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(11314..11856)
                     /gene="yfcD"
                     /locus_tag="RH057_001596"
     CDS             complement(11314..11856)
                     /gene="yfcD"
                     /locus_tag="RH057_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HDV3127983.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSEVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(11914..12465)
                     /gene="yfcE"
                     /locus_tag="RH057_001597"
     CDS             complement(11914..12465)
                     /gene="yfcE"
                     /locus_tag="RH057_001597"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HDV3127984.1"
                     /translation="MKLMFASDIHGSLPATERVLELFAQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMLLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPASYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(12521..13165)
                     /gene="yfcF"
                     /locus_tag="RH057_001598"
     CDS             complement(12521..13165)
                     /gene="yfcF"
                     /locus_tag="RH057_001598"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HDV3127985.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLVLGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            13301..13948
                     /gene="yfcG"
                     /locus_tag="RH057_001599"
     CDS             13301..13948
                     /gene="yfcG"
                     /locus_tag="RH057_001599"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HDV3127986.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFESGAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRQRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            14005..14367
                     /gene="folX"
                     /locus_tag="RH057_001600"
     CDS             14005..14367
                     /gene="folX"
                     /locus_tag="RH057_001600"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HDV3127987.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            14388..15281
                     /locus_tag="RH057_001601"
     CDS             14388..15281
                     /locus_tag="RH057_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HDV3127988.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHEQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPIRNEQFAHALGHALHRPAILRVPATAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(15329..16231)
                     /gene="rpnB"
                     /locus_tag="RH057_001602"
     CDS             complement(15329..16231)
                     /gene="rpnB"
                     /locus_tag="RH057_001602"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HDV3127989.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARDFIDIHLPAPLRKLCDLTT
                     LKLEPNSFIDDDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEIAERAPQEKEKLMTIADRLREEGRNDGLILGKREEALRIAQEMLD
                     RGLDRELVMMVTRLSPDDLIAQSH"
     gene            complement(16428..17201)
                     /gene="hisP"
                     /locus_tag="RH057_001603"
     CDS             complement(16428..17201)
                     /gene="hisP"
                     /locus_tag="RH057_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HDV3127990.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(17209..17925)
                     /locus_tag="RH057_001604"
     CDS             complement(17209..17925)
                     /locus_tag="RH057_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3127991.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(17922..18608)
                     /gene="hisQ"
                     /locus_tag="RH057_001605"
     CDS             complement(17922..18608)
                     /gene="hisQ"
                     /locus_tag="RH057_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HDV3127992.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNTVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(18698..19480)
                     /gene="hisJ"
                     /locus_tag="RH057_001606"
     CDS             complement(18698..19480)
                     /gene="hisJ"
                     /locus_tag="RH057_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HDV3127993.1"
                     /translation="MKKLVLSLSLVLAFSSATAAFAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIDLAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(19701..20483)
                     /gene="argT"
                     /locus_tag="RH057_001607"
     CDS             complement(19701..20483)
                     /gene="argT"
                     /locus_tag="RH057_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HDV3127994.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYAPFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDDAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(20749..21318)
                     /gene="ubiX"
                     /locus_tag="RH057_001608"
     CDS             complement(20749..21318)
                     /gene="ubiX"
                     /locus_tag="RH057_001608"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HDV3127995.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVADIETHLVMSQAARQTL
                     SLETGFSLREVQALADVTHDARDIAASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(21413..22930)
                     /gene="purF"
                     /locus_tag="RH057_001609"
     CDS             complement(21413..22930)
                     /gene="purF"
                     /locus_tag="RH057_001609"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HDV3127996.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(22967..23455)
                     /gene="cvpA"
                     /locus_tag="RH057_001610"
     CDS             complement(22967..23455)
                     /gene="cvpA"
                     /locus_tag="RH057_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HDV3127997.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIRGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(23714..24376)
                     /gene="dedD"
                     /locus_tag="RH057_001611"
     CDS             complement(23714..24376)
                     /gene="dedD"
                     /locus_tag="RH057_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HDV3127998.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPTTIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEAPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(24366..25634)
                     /gene="folC"
                     /locus_tag="RH057_001612"
     CDS             complement(24366..25634)
                     /gene="folC"
                     /locus_tag="RH057_001612"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HDV3127999.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGREKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(25704..26618)
                     /gene="accD"
                     /locus_tag="RH057_001613"
     CDS             complement(25704..26618)
                     /gene="accD"
                     /locus_tag="RH057_001613"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HDV3128000.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDVLKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQALEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPGFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(26774..27433)
                     /locus_tag="RH057_001614"
     CDS             complement(26774..27433)
                     /locus_tag="RH057_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3128001.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VTPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(27516..28328)
                     /gene="truA"
                     /locus_tag="RH057_001615"
     CDS             complement(27516..28328)
                     /gene="truA"
                     /locus_tag="RH057_001615"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HDV3128002.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(28328..29341)
                     /locus_tag="RH057_001616"
     CDS             complement(28328..29341)
                     /locus_tag="RH057_001616"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HDV3128003.1"
                     /translation="MSEGWNIAVLGATGAVGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(29407..30543)
                     /gene="pdxB"
                     /locus_tag="RH057_001617"
     CDS             complement(29407..30543)
                     /gene="pdxB"
                     /locus_tag="RH057_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HDV3128004.1"
                     /translation="MKILVDENMPYARDLFSRLGEVTAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQHADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGPLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            30642..31637
                     /gene="flk"
                     /locus_tag="RH057_001618"
     CDS             30642..31637
                     /gene="flk"
                     /locus_tag="RH057_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HDV3128005.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNNRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLAAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(31634..32812)
                     /locus_tag="RH057_001619"
     CDS             complement(31634..32812)
                     /locus_tag="RH057_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3128006.1"
                     /translation="MTAVSQTETRSSANFSLFRIAFAVFLTYMTVGLPLPVIPLFVHH
                     ELGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVVGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPVLAWACNGTVRKVPALAEERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(33105..34325)
                     /gene="fabB"
                     /locus_tag="RH057_001620"
     CDS             complement(33105..34325)
                     /gene="fabB"
                     /locus_tag="RH057_001620"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HDV3128007.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNVKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAHIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMKMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKD"
     gene            34484..36490
                     /gene="mnmC"
                     /locus_tag="RH057_001621"
     CDS             34484..36490
                     /gene="mnmC"
                     /locus_tag="RH057_001621"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HDV3128008.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVVAESGFGTGLNFLTLWQAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDEGRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQDAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSAPWFNRTGSSKREAAIIGGGIAS
                     ALLSLALLRRGWQVTLYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWNEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSHTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
BASE COUNT         9025 a         9946 c         9097 g         8482 t
ORIGIN      
        1 acagataaaa aaaccatact tacaattaag aatcagaaca attcactata taacattgca
       61 tgtaaagcat atacacctca ttattttgtc attattaagt tattaacagc acaatcgagc
      121 cttcccctct ggcaaaatct tattctgcag accttcaaaa caccgtcctg ggggagtaca
      181 ttgttctaag ctgacttcca cggcagggag tggcgataac agcaaaaaag gtcaagattc
      241 atgtccccca ttgaaaaatc cagcaaatta gagaatgtct gttatgacat ccgtggtccg
      301 gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa actgaacatc
      361 ggcaacccag ccccgttcgg ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc
      421 aacctgccta ccgctcaagg gtattgcgat tccaaaggtc tttactccgc gcgtaaagcc
      481 atcatgcagc actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc
      541 ggcaatggtg tatcggagct tatcgttcag gcaatgcagg cattgctgaa cagcggggac
      601 gaaatgttgg ttcctgcacc agattaccca ctatggaccg cggcggtttc gctttccagc
      661 ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact ggttcccgga cctcgatgat
      721 attcgcgcta aaattacgcc tcgtacgcgt gggatcgtta taatcaaccc aaataaccca
      781 accggcgcgg tatattccaa agagctttta atggagattg tggagattgc acgtcagcat
      841 aatctcatta tcttcgccga tgaaatttat gacaaaattc tttacgacga cgctgagcat
      901 cactcaattg cgccgctggc acctgacctg ctgaccatta cctttaacgg actgtcgaaa
      961 acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac
     1021 gccaaaggct acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt
     1081 cctgcgcaac acgccattca gaccgcgctg ggtggttatc agagcatcag tgaatttatt
     1141 acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt gggaactgat caacgatatt
     1201 ccgggcgttt cctgcgtgaa acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc
     1261 aaacgcttta acattcacga cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa
     1321 gttctgttgg tgcaagggac ggcattcaac tggccgtggc cggatcactt ccgcattgtc
     1381 acgctaccgc gtgtcgatga tatcgagctg tctttgagca agttcgcgcg tttcctttct
     1441 ggttatcatc agctgtaatc ttaatttcac tgccggagat tgcatccggc agcgttatcc
     1501 cgccacaatg acctgatgat gtcatcatac gtaaggtcac tatgaaacag agccattttt
     1561 ttgcccatct ctcccgcctg aaactcatta accgctggcc gctcatgcgc aacgtgcgga
     1621 cggaaaatgt gtccgaacac agtttgcagg tagcgatggt cgcccatgcg ctggcagcta
     1681 tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt
     1741 accacgatgc ctcagaagtg ctcaccggcg atctccctac tccggtgaaa tacttcaatt
     1801 cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc tcagcaaaaa ctggtcgata
     1861 tggttccgga agagctgcgg gatatctttg cgccgttaat tgacgagcat gcatatagcg
     1921 atgaagaaaa atcgctggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg
     1981 aagaactcgc ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc
     2041 ttgaagcgcg tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc
     2101 atctttcgct cgatgagatt agccaggatt caccgctgta agcagccgga gtctgcgtcg
     2161 catcaggcaa taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg
     2221 gatcatcacc acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa
     2281 gtcactgaag ctgtaatttc ccggacctaa aaccagtgtg ttaacaggtg aagaaaccgg
     2341 tgtcataaag gcggcggatg ctgccatcgc cacgaccatc gcgaatggat aaggcgacac
     2401 ccccatcgtt ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt
     2461 agagataaat agcccaataa ccgccgacaa gacaaacaaa caacccagca tcatatgtgg
     2521 cccgtaacca ccgccaatgt ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt
     2581 ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat
     2641 ggatttatag gcgctttcag catctataca gcggaatttc cccatcagca ggcaggcgat
     2701 gatagcggca acaggattag gaatttcatc tgtcagcatt aacgccacca ttagcaccag
     2761 acagaaaatg gcatggggtg cctggctgtg cgcgggcgat gcttcactca cctcttccgg
     2821 taagttcagc gctacgaagt cgcggccctg tttggccagc ataccgatca gtttccagtt
     2881 acccacaacc aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag
     2941 cgccacgcca ttgcgcttta gccccaccac attcagtccg tagcgggtac gaaaaccaat
     3001 ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc
     3061 cacatcaagg gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg
     3121 ctcactacaa aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc
     3181 acgcgcgcga aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca
     3241 gcgttcaaca ccgatgacgt tagcgccata acgctcacgt aatttgagat catccagccg
     3301 ttgacctatc attggcgatc cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg
     3361 atattcacgg ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg
     3421 ggtatcccct ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac
     3481 gaccaggcca ataggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa
     3541 ttcactgttg actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag
     3601 cccggcaaaa cttaacggca tcatcagacg tgacggcgaa gtttgcatac gcatggcaac
     3661 gcttaacacc acgggaataa agatagcgac aacgccggtt gaactcataa acgcgccaag
     3721 ccccgcgacg gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgccacttt
     3781 gaccagccat gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat
     3841 aaacaaggcg gcaatcagga caacgttagg atcagaaaag ccggaaaata cttcggggac
     3901 cgtcagcgtt ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg
     3961 cactctgccc gtcgcaaaca agacgatggc aacggccagt aatgaaagaa cccaaatcaa
     4021 ttcaccgttc acaacttatc cttgttaatt gagggggatg acttgattct gccataaaaa
     4081 agccccgacg agacggggct aaatcatgat caggtgtttc actgaataat aacatcgcca
     4141 tttggctgtt tggtcacagt aatttgctcc agactgtgga ggaccaaatc gacctcattc
     4201 aggcgcgggg tatctgccgg agcgttaacc gcaatgacat ggcaacccgc cgccaggcca
     4261 gaaagcacgc cagcgggagc atcttccacc accacacact cctgcggcgc aagccccagc
     4321 agctgcgcac ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg
     4381 gttacaaaca cctctggtgc gggaagccca gctattttat ggcgcgctcg cgctaccggc
     4441 atggagccag aagtcacaat ggcccacgga atacctgctt tattcaaatg attgagtaag
     4501 gcgattgccc ccggaagcgc ggtaatacct tcggtttccg tggcctcgat gtgctccaga
     4561 cgcgtaaact cggcggcaat atcagcctcg gatttgcccg ccataaaatg gcgcaaagag
     4621 gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa cccatgacgt
     4681 ctggcccagt tgctccacgc ccgttctacc gcaggcaggg aatccaccag cgttccatca
     4741 agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct caggcgttga taatttgatt
     4801 aatttcgttg gcgctcaaat ggtactgacg cgggcaggca tgccacacat taagcatgcg
     4861 ctgatatttt tcccacattg gcgtctgggc gttaaaaccg tgagttccgg cgtcaaaatg
     4921 ggtatagcgc ccttccacat taaccataaa gcggacataa ccgaggtaac gtgcttcagt
     4981 ggcggcgtca aagccgagga aggtgacacg acgttcatcg atggattgct ggtcctgcaa
     5041 attagaccag gaaacatgca acgcatgata catctccata atgtcgatga tagtgcggca
     5101 ggtttcttct ttcagctcgc caaactcgcg atccaattca cgcatctgta atccgtaacc
     5161 acgctcaata attgtttgca ggcgacggta acgttcagca tttgccggat cgagcatagt
     5221 catcatcttg tactgattag acaaaataag acgttgcgcg ttggtcattt ccattgttga
     5281 ctcctgtatc actatactac ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg
     5341 cgtaatcagg ggtcaattac aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc
     5401 gcttaagcgt gtcagctaat gcctggtaat caggcttgcc ttcaacgggt gccaacacct
     5461 gtccagactc ctgcaattta ccgcgaactt cataaaacca gttaaggatt gcagggggta
     5521 atggcgttac agaacgcttg cccagccacc acaatccctg catgggtaaa cttaaggcga
     5581 acagcgcagt ggcaactgcc ggcccaagct gaccgcccag ggcaatctgc cagcagagag
     5641 taaatacggc gatcggcggc ataaaacgga tcgcataacg cgtcatcttg ataacgcgat
     5701 tttcgacaaa gaccggggca aggcgttttt ccagcggcca cgtctttgag taatgctgtc
     5761 cccggcgaaa caagctaaaa aaattaacag aacgattatc cggcgttgac atgcttcacc
     5821 tcaacttcac atataaagat tcaaaaattt gtgcaaattc acaactcagc gggacaacgt
     5881 tcaaaacatt ttgtcttcca tacccactat caggtatcct ttagcagcct gaaggcctaa
     5941 gtagtacata ttcattgagt cgtcaaattc atatacatta tgccattggc tgaaaattac
     6001 gcaaaatggc atagactcaa gatatttctt ccatcatgca aaaaaaattt gcagtgcatg
     6061 atgttaatca taaatgtcgg tgtcatcatg cgctacgctc tatggctccc tgacgttttt
     6121 ttagccacgt atcaattata ggtacttcca tgtcgagtaa gttagtactg gttctgaact
     6181 gcggtagttc ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa gagtaccttt
     6241 ctggtttagc cgaatgtttc cacctgcccg aagcacgtat caaatggaaa atggacggca
     6301 ataaacagga agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg
     6361 ttaatactat tctggcacaa aaaccagaac tgtctgcgca gctgactgct atcggtcacc
     6421 gtatcgtaca cggcggcgaa aagtatacca gctccgtagt gatcgatgag tctgttattc
     6481 agggtatcaa agatgcagct tcttttgcac cgctgcacaa cccggctcac ctgatcggta
     6541 tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa aaacgttgct gtatttgaca
     6601 ccgcgttcca ccagactatg ccggaagagt cttacctcta cgccctgcct tacaacctgt
     6661 acaaagagca cggcatccgt cgttacggcg cgcacggcac cagccacttc tatgtaaccc
     6721 aggaagcggc aaaaatgctg aacaaaccgg tagaagaact gaacatcatc acctgccacc
     6781 tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa atgcgttgac acctctatgg
     6841 gcctgacccc gctggaaggt ctggtcatgg gtacccgttc tggtgatatc gatccggcga
     6901 tcatcttcca cctgcacgac accctgggca tgagcgttga cgcaatcaac aaactgctga
     6961 ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag cgactgccgc tatgttgaag
     7021 acaactacgc gacgaaagaa gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg
     7081 cgaaatacat cggtgcctac actgcgctga tggatggtcg tctggacgct gttgtattca
     7141 ctggtggtat cggtgaaaat gccgcgatgg ttcgtgaact gtctctgggc aaactgggcg
     7201 tgctgggctt tgaagttgat catgaacgca acctggctgc acgtttcggc aaatctggtt
     7261 tcatcaacaa agaaggtacc cgtcctgcgg tggttatccc aaccaacgaa gaactggtta
     7321 tcgcgcaaga cgcgagccgc ctgactgcct gatttcacac cgccagctca gctggcggtg
     7381 ctgttttgta acccgccaaa tcggcggtaa cgaaagagga taaaccgtgt cccgtattat
     7441 tatgctgatc cctaccggaa ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg
     7501 tgcaatggaa cgcaaaggcg ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac
     7561 cggtggcgat gcgcccgatc agactacgac tatcgtgcgt gcgaactctt ccaccacgac
     7621 ggccgctgaa ccgctgaaaa tgagctacgt tgaaggtctg ctttccagca atcagaaaga
     7681 tgtgctgatg gaagagatcg tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt
     7741 tctggttgaa ggtctggtcc cgacacgtaa gcaccagttt gcccagtctc tgaactacga
     7801 aatcgctaaa acgctgaatg cggaaatcgt cttcgttatg tctcagggca ctgacacccc
     7861 ggaacagctg aaagagcgta tcgaactgac ccgcaacagc ttcggcggtg ccaaaaacac
     7921 caacatcacc ggcgttatcg ttaacaaact gaacgcaccg gttgatgaac agggtcgtac
     7981 tcgcccggat ctgtccgaga ttttcgacga ctcttccaaa gctaaagtaa acaatgttga
     8041 tccggcgaag ctgcaagaat ccagcccgct gccggttctc ggcgctgtgc cgtggagctt
     8101 tgacctgatc gcaactcgtg cgatcgatat ggctcgccac ctgaatgcga ccatcatcaa
     8161 cgaaggcgac atcaatactc gccgcgttaa atccgtcact ttctgcgcac gcagcattcc
     8221 gcacatgctg gagcacttcc gtgccggttc tctgctggtg acttccgcag accgtcctga
     8281 cgtgctggtg gccgcttgcc tggcagccat gaacggcgta gaaatcggtg ccctgctgct
     8341 gactggcggc tacgaaatgg acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac
     8401 cggcctgccg gtatttatgg tgaacaccaa cacctggcag acctctctga gcctgcagag
     8461 cttcaacctg gaagttccgg ttgacgatca cgaacgtatc gagaaagttc aggaatacgt
     8521 tgctaactac atcaacgctg actggatcga atctctgact gccacttctg agcgcagccg
     8581 tcgtctgtct ccgcctgcgt tccgttatca gctgactgaa cttgcgcgca aagcgggcaa
     8641 acgtatcgta ctgccggaag gtgacgaacc gcgtaccgtt aaagcagccg ctatctgtgc
     8701 tgaacgtggt atcgcaactt gcgtactgct gggtaatccg gcagagatca accgtgttgc
     8761 agcgtctcag ggtgtagaac tgggtgcagg gattgaaatc gttgatccag aagtggttcg
     8821 cgaaagctat gttggtcgtc tggtcgaact gcgtaagaac aaaggcatga ccgaaaccgt
     8881 tgcccgcgaa cagctggaag acaacgtggt gctcggtacg ctgatgctgg aacaggatga
     8941 agttgatggt ctggtttccg gtgctgttca cactaccgca aacaccatcc gtccgccgct
     9001 gcagctgatc aaaactgcac cgggcagctc cctggtatct tccgtgttct tcatgctgct
     9061 gccggaacag gtttacgttt acggtgactg tgcgatcaac ccggatccga ccgctgaaca
     9121 gctggcagaa atcgcgattc agtccgctga ttccgctgcg gccttcggta tcgaaccgcg
     9181 cgttgctatg ctctcctact ccaccggtac ttctggtgca ggtagcgacg tagaaaaagt
     9241 tcgcgaagca actcgtctgg cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct
     9301 gcagtacgac gctgcggtaa tggctgacgt tgcgaaatcc aaagcgccga actctccggt
     9361 tgcaggtcgc gctaccgtgt tcatcttccc ggatctgaac accggtaaca ccacctacaa
     9421 agcggtacag cgttctgccg acctgatctc catcgggccg atgctgcagg gtatgcgcaa
     9481 gccggttaac gacctgtccc gtggcgcact ggttgacgat atcgtctaca ccatcgcgct
     9541 gactgcgatt cagtctgcac agcagcagta atctcgtcat catccgcagc tttgcgctgc
     9601 ggatatctga accggaaata atcactattt ccggtttttt attctcttaa tctgcattaa
     9661 tcctttctga ttatcttgct taactgcgct gcatcaatga attgcgccat ttcactttgc
     9721 atacttacca ctttgttttg tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc
     9781 caaaccaaca agaagatggg caatgcccga tacgttggtg attatctttt ttgttgctat
     9841 tttaaccagc cttgccacct gggtagttcc ggtggggatg tttgacagtc aggaagtgca
     9901 gtatcaggtt gatggtcaaa caaaaacacg caaagtcgta gatccacact catttcgcat
     9961 tctgactaac gaagcaggcg aacctgagta tcaccgcgta cagctgttca cgacgggcga
    10021 tgaacgcccg ggcctgatga acttcccgtt tgaaggatta acctcaggat cgaaatacgg
    10081 gacagctgtt ggcatcatca tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat
    10141 gcgtacagga accattgata acggtatcct ggcgcttatt cgccataccc gcgggaatga
    10201 aattctcttt attcctgcgc tgtttattct gttttcactt ggcggcgcgg tatttggtat
    10261 gggagaagag gccgtcgcct ttgccattat catcgcaccg ctaatggtcc ggctgggcta
    10321 tgacagtatt accaccgtcc tggtgaccta tattgccacg caaatcggtt ttgccagttc
    10381 gtggatgaac ccgttttgtg tggtcgttgc tcaggggatt gccggcgttc cggtgctttc
    10441 tggctccggg ttgcgcatcg tggtgtgggt tatcgccact ctgattggcc tgatctttac
    10501 catggtgtac gcctcacgag tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga
    10561 ccgcttcttt cgtgaaaagc aggctgatgt tgaacaacgt ccgtttacct ttggtgactg
    10621 gctggtattg attgtcctga ccgccgtaat ggtctgggtg atttggggcg tgatcgttaa
    10681 tgcctggttt attccagaaa ttgccagcca gttcttcacc atgggtctgg tgattggcat
    10741 catcggcgtt gttttccgcc ttaacggcat gacggttaat accatggctt catcctttac
    10801 cgaaggggcg cgaatgatga tcgcccctgc cctgctggtg ggtttcgcca aagggatttt
    10861 gctgctggtc ggtaatggtg aagcaggtga tgccagcgtg ttaaatacca tcctcaacag
    10921 cattgccaat gccattagcg gtctggacaa cgctgtcgcg gcctggttta tgttgctctt
    10981 ccaggcggta tttaatttct ttgtgacatc cggttctggt caggcggcgt taaccatgcc
    11041 gttactggca ccgcttggcg atctggtcgg tgttaaccgt caggttaccg tgctggcatt
    11101 ccagtttggt gatggcttca gccacatcat ttacccaacc tcagcttcgt taatggcgac
    11161 gctcggtgtt tgccgggtgg acttccgtaa ctggctgaag gtgggcgcga cactgcttgg
    11221 actgctgttt attatgtcca gcgtcgtggt gatcggcgct cagttgatgg gctaccacta
    11281 aaaatgttaa gagccgcaat tgcggctctt ttttcattct gccgtttcag tctctacggc
    11341 ttcatttttg gcattgcgct tcatccacaa cgccagcgct ttcagcgagt ctggagtgaa
    11401 ctcatcgcag cgtgcggtga tttcttccgg cgtcagccag caaacttcac tgacttcatc
    11461 ttcctgtagg gcgaagggac cgtgagagac gcagctgaac aatgcgcccc agacacggca
    11521 atttttatct tcgaaataga actgcccgtg ctcagcaaag gggacaccgg caatgcccaa
    11581 ctcttcttcc gcttcgcgac gcgcggattc cagcagttgc tcatcggcct ggaccacacc
    11641 gcctgcggtc gcatctaaca tgccgggtaa aaagtctttt gtctcggtac gacgctggac
    11701 cagaattttg cccatgccat catgcacgac gatgtaagtt gcacgatgac gcagacactg
    11761 tgcccgcatt tgttcccggc tggcttgtgc aatgacttcg ttctcttcat tgacaatatc
    11821 cacccattca gtacttgcca aacgacgctg ttccattatc aggaaacctt ctttttctgg
    11881 cgctcttacg gcgcattttg agttgtgggt aaattacgga ttaatcgcga cctgcgcaat
    11941 gatactttga tcattgagtg cgattacact aagtacgtca ttatccagca tgccataact
    12001 cgccggatta ccgcctttcg gaatactcac cgagccgggg ttgaagtgga aaatctcccc
    12061 ccgctgttct gccactggca gatgggtatg gccgtacacc agcacatcgt tctggtttaa
    12121 agcaggcaga ttttccgggc caaaaagatg accatgcgtc aaaaacagac gttgtttttc
    12181 cagtaatacc tgttgccacg gcgcggttat cgggaaatgc agcagcattt gatccacttc
    12241 gctgtcgcag ttgccgcgca cagcgataac cttatgtgcc acttcattaa gccgttcagc
    12301 gactttggct ggcgcgtaac cctccggtaa agcattacgc gggccgtgat tcaacacgtc
    12361 gccaaggatc accagccact gggcaccgct ttgggcaaac aactccagaa cacgttccgt
    12421 cgccggtaac gacccatgaa tgtccgatgc aaacatcagt ttcatcactc actcctcgtc
    12481 gaaaaaacgt aagctatgat actggattct gtcgttgcta tcagccagat tgcttcgccg
    12541 aaagtgcaat aaaacgctgg acagacgctc gctgccactg gaatgtcgca taatccacca
    12601 ggcgttctgg cacctcatcg ccatgtagta ccaggcggtt aatcattagc gccagatcag
    12661 tatcagcaat gcaccattca ccaaataaat tcggctgacc cagtactaac aaatgttctg
    12721 ccatcgcaaa cagtttctct gcactggctt ttccctcggc cgttagtggc gcttttttcg
    12781 cccccgcaaa gacaacatcc gtcggacgct cttcgcggat gggcatcaga tcgctgcgca
    12841 gccaggcctg aatctgtcgt gcacgcgcac gattttctaa atcaagcgga taaatacgtt
    12901 cccaggtcgg tggcgcaaat cgatcttcca gatactccgc aatggcagaa gattcactca
    12961 actcaaaatc atcgatttgt aataacggca cacggcgtgt ctgaccgtaa ccttgccacg
    13021 tcggctgcaa atgttcaccg ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt
    13081 tttcctgcaa cgccacccag gcggataaca cataagggga gaaaaagtgg gcatctgacc
    13141 aaagcgtgat agcgggttta ctcattatgt cctcggcaat aaagggggct ggctatcaaa
    13201 ctatagagcc tttttgtcac tgtcacgtga cgaatactca cgtaagtcgc cgcgctttct
    13261 atacttgtgg tgttcagatg attaaacaac cggagctgca atgatcgatc tctatttcgc
    13321 cccgacaccc aatggtcaca aaattacgct gtttctcgaa gaagcagagc ttgattatcg
    13381 cttgattaag gtagacctgg ggaaaggcgg tcagtttcgt ccggaatttt tgcgcatttc
    13441 gcccaacaac aaaattccgg caattgttga tcattctcct gccgatggcg gcgaaccgtt
    13501 aagcctcttt gagtctggtg ccattttgtt gtatctggct gagaaaacag gactcttttt
    13561 gagtcatgaa acgcgtgagc gcgccgccac attacagtgg ttattctggc aggtaggcgg
    13621 actggggccg atgcttgggc aaaatcatca ttttaatcac gcagcccccc aaaccattcc
    13681 ttacgctatt gaacgttatc aggttgaaac tcagcgtctt taccatgtac tgaacaagcg
    13741 gctggaaaac tcgccctggc tgggaggcga gaactacagc attgcggata ttgcctgctg
    13801 gccgtgggtt aatgcctgga ctcgccagcg aattgacctc gcaatgtatc cggcagtcaa
    13861 gaactggcat gagcggatcc gttcgcgccc tgccaccggg caggcactgc taaaagcaca
    13921 actcggtgat gagcgttcgg atagttaaca gaaacaggtt ctcgtgtatt atttcatcct
    13981 aagtaaaaca acggagaacc tgcaatggca caacctgccg ctattattcg tataaagaac
    14041 cttcgtttgc gtacgtttat cggaattaag gaagaagaaa ttaacaaccg tcaggatatt
    14101 gttatcaatg tgacgatcca ctaccccgcc gataaagcgc gcactagcga agatatcaac
    14161 gatgcgctga attatcgcac cgtaacgaaa aacattattc agcatgtaga gaataaccgt
    14221 ttctctttgc tggaaaaatt aactcaggat gtgctcgata tcgcacgtga acatcactgg
    14281 gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta
    14341 tccatgacct taagctggca gcgttaaccg ccatatcggg aggctgcatg aacatagtga
    14401 tcaccggagg gacgggatta attggtcgcc atttgattcc gcgtttgctg gagctgggcc
    14461 atcaaatcac ggtagtgacg cgtaacccac agaaagccag ttccgttctc ggccctcggg
    14521 tgacgctatg gcaagggcta gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca
    14581 acctggccgg agaaccgatt gctgataaac gctggactca cgagcaaaaa gagcgtctct
    14641 gccaaagccg ctggaatatc acgcaaaaac tggtcgattt gattaatgcc agcgacacgc
    14701 cgccgtcggt actcatttcc ggctcggcaa cgggctatta tggcgactta ggtgaagtgg
    14761 tggttaccga agaggaaccg ccgcataacg aatttactca taaactctgc gcccgctggg
    14821 aagagattgc ctgccgggcg caaagtgaca aaacgcgagt gtgcctgctg cgtaccggcg
    14881 tagtgctggc accggatggc ggtattctcg gtaaaatgct gccgccgttt cgtcttggcc
    14941 tgggcgggcc gattggttcc ggtcggcagt atctggcctg gattcatatc gatgatatgg
    15001 tcaacggcat tctctggctg ctggataacg agctgcgcgg gccatttaat atggtttcgc
    15061 cctacccgat acgcaatgaa caatttgccc atgcgctcgg tcatgcgctg catcgcccgg
    15121 ctattttgcg cgtccccgca accgccattc ggctgttaat gggcgaatct tcagtactgg
    15181 tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga agcgggtttt gcgtttcgtt
    15241 ggtacgattt agaagaggcg ctggcggatg tcgttcgctg atgtggttta ctgcaaacat
    15301 ccgccagtta actcccggtg ttacagggtt agtggctttg cgcgataaga tcgtctggtg
    15361 aaagtcgggt caccatcata actaactctc ggtctaaacc tctatccagc atctcctgag
    15421 caatacgcag ggcttcttcg cgtttgccaa gaattaatcc atcattccgc ccttcttcac
    15481 gtaatctgtc agcaatggtc atcagtttct ccttttcctg tggtgcgcgt tctgctatct
    15541 caccaataaa tgcacggaaa cgctgggcat ctcccgtttg taatacataa ttaaacaggg
    15601 cttttagctg tctgtcatta gtgttccctg taactagcag cgaaacaatt tggtcgacta
    15661 atcccaacag atcgcgctga cgaatatgtt tctgaattaa ctccaacagc gccattttgc
    15721 gatgttgcat aatctcgtca tccggcacca cggtaatatc caccaacgga aaagccgatg
    15781 aatatatttt acgggcaatc gcaggctcgg caaattcatc aagccagcag agtgaataag
    15841 gataaggact tctgcaacca tgataaaaca gcattgggat caccaatgga agctctttat
    15901 agcctgcatc cagatggttt tgcatcgccg cgatggaata gcgcatcatg cgaaaagcca
    15961 ttaattcttc cggcttactt tggtgctcta tcactacata aatataaccc actccctcct
    16021 gcgttttcac agaccacaag aggtcggaat aatattgccg caggtcgtca tcaataaaac
    16081 tgtttggttc cagtttaagc gtcgttaaat cacacagttt gcgcagcggc gcgggaagat
    16141 gaatatcaat aaaatcccgc gcggtgtctg gatggcgtaa aaaagattta aataccgcat
    16201 catgcggcgt ggaagttgtc gatattgtca tggcgttccg tcacccctca aatttaagat
    16261 gacgcgacaa taaccacgat taacacccaa tgcagcaggc agtttttatc tttggacagc
    16321 gaattcagaa gtatttttag cggactgcaa cttttacatt tcagcggaag ctgactcgca
    16381 aattccccat gaaaatactc cggcagcgtt agccaccgga gaggaaatta tttcagcgat
    16441 cccttaagga actgttgcag acgagggctt tgcgggttgc caaataactg ctccggcgcg
    16501 ccctcttctt ctattttccc ctgatggagg aaaatgacgt gagtagaaac atggcgagca
    16561 aagcccattt cgtgagtcac taccaccatg gttttcccct cttctgccag ttgctgcata
    16621 atacgtaaca cttcgcctac cagttcagga tcgagcgccg aggtaggttc atcaaacagt
    16681 aaaacttccg gttccatcgc cagcgcccgc gcgatagaaa cacgctgttg ctgaccgccg
    16741 gaaagatgca ccggatattt cccctgcgca cgttcgtcta tcccgacttt tgccagatac
    16801 ttcaccgccc gctcgcgcgc ttcctgcttg ctcaggccca acacctgaat cggcgcttcc
    16861 atgacgtttt ccagcaccgt catatggctc cagagattga agtgctggaa taccatcgtc
    16921 aggcgtgtgc gcagtaagcg cagttgattt ttatcggcga ctttgagttg accgtctttg
    16981 tcgcgcacca gattgatcgt ctggccattg accacgatcg acccttcact cggtttttcg
    17041 aggaagttaa tgcagcgcag aaaggtactt ttccccgatc ccgacgatcc gatgatgctt
    17101 attacatctc cggcattcgc ttgcagtgag acccctttca gcacttcatg ttcgccgtag
    17161 cgtttgtgca aatcgataac gtttaattta ttctcggaca tcgtattctc agtgcgttga
    17221 agaaggtttc acatgctgca accagcgttt ttccgctctg cgaaagaggc tgatcaggac
    17281 ataagagatg attaaataga gcaccgcggc aatgccgaag gcggtaaaag gttgatacgt
    17341 ggcggcgtta atatcgcggg ctattttcag cagatccggc acagtggccg taaacgccag
    17401 cgcagtagag tgcagcatca ggatcacttc gttgctgtat gccggtaacg caatacgcag
    17461 cgcagaaggc aaaataatgc aacgatacat tttaaaagtc gagaagccat aggctctggc
    17521 ggcttcaatt tccccatgcg gaaccgaacg gattgcccca gcaaaaatct cggtagtgta
    17581 agcgcaggtg ttaagcgtca gcgccagcac ggtacagttc aggccactgc ggaagaaagc
    17641 gttaaggaat tcggttccct taacaatctc aagcgtgtac atgccggaat agaacaccag
    17701 caactgaaca tacagcggcg taccgcgaaa aatataggta aataaccaga ttggaaactg
    17761 gatgtattta ttactggaga cacgaccaat cgccagaaac agcgccagga ctccgcctat
    17821 cactaccgac aaaataagca gccacagagt gatcgccaca ccagtaaagc gataaccgtc
    17881 ggtccacagc agcggtttcc agtattcatg taagatttcg atcacaggtc agccctcttc
    17941 acacccacgg agtagcggcg ctcaaggaac agcagcacac cattggaaac agtggtgaaa
    18001 accaggtaaa tcacgccaca gacgatggcg aaatagaacg gttcccaggt acttttgcct
    18061 gccagttggg tggctttgac cacatcttcc aggccgagta acgaaaccag tgcggtagat
    18121 ttgaggatca cctgccagtt gttgccaatg cctggcaggg cgtaacgcat catcgccgga
    18181 aacatgatcc gccgaaacac ttgcccacga gtaaaaccga acgccgtcgc cgcctctata
    18241 tgccctttcg gcactgccat aaacgcgcca cggaacgttt cggtaaaata agcaccgtag
    18301 ataaaaccga gagtaataat accagcgacc atcggatcga tatcaatctg cccgacgccc
    18361 atcgcctccg tcaccgtgtt tagtgcaatc tgcaaaccgt agaaaatcag cagcatcaac
    18421 accaagtccg gcacgcctcg aatcagcgtg gtgtaccctt cgaaaatcag ccccgaaagc
    18481 cgattttgcg agagcttacc gccagcgcca attaaaccga tgattacagc gagcactaca
    18541 gaactgatag ccagctccag cgtgacgagc gcaccctgta aaataacacc tgaaaaccca
    18601 tacaacatgc tgcctgtcct gtcgtgtgtg gtgaattacc gcctcttgtc tccctccggt
    18661 tacccgaagg gagcgggctt tctttcaccg atggcgatta gccaccataa acatcaaaat
    18721 cgaagtactt tttcgctaat ttctcgtaag taccgtcagc gcgcatttcg gcaaaggctt
    18781 tgttcagtgc ctcgcgcagt tcgttatctt ctttacgcag gcccatgccg gtccctacgc
    18841 caaacagttt ttcatcttta acagacgggc caccgaattt gtaatcttta ccgacaggtt
    18901 gtttgaggaa accttcgctg gcagcgacct catcctggaa cgcggcatca atacgtccgg
    18961 cagtcaggtc agaataaatg ttgtcctgcc cctgatacga gacgatttca atgccttttg
    19021 gtgcccaatg ttcgttaccg aacgtctcct gggtggtgcc ctgcaatacg cctacccgct
    19081 tgcctttcag cgactcgact gtcggctgaa tgtcggaatt tttcgccacc accagacgag
    19141 aatcggcagc gtacagtttg tcggtgaagg caatttcttg ctgacgtttt tccgtaatgg
    19201 aaagcgatga catgatggcg tcaatcttct tcgcttttaa ggacgggatt aacgcatcca
    19261 gcggattttc gacaaacgta cattgcgtat tgatgcgttt gcataattcc tttgccagat
    19321 cgatatcgaa gccaaccagt tcgccttgtg aattttttga ttcaaatggc gcataggtcg
    19381 ggtcggtacc gatgcggatg ttttgcggaa tcgcagcaaa cgccgcagtt gcgctggaga
    19441 aggccagaac cagagagagc gatagcacca gttttttcat aatgtcctca actgacagtc
    19501 tttttatagg gaatatttac aggtttgtta cacttatcgt gccataaaat tgaccatcaa
    19561 ggcaaaatat cccgccccgt tagacgtatt atttttcatt aaaaatgctt aagtatgcat
    19621 ttcaatgcac cacgatagtg caacaaacct ggcgtgcacc atgatgatca ttccatcagg
    19681 tacagcttcc cagcgacgta tcagtcaccg tagacattaa agtcgaaata ctttttcgcc
    19741 atcttgtcgt aggtgccgtc ctgacgcagc tcgccaagcg ccttattgaa ggcagccgtc
    19801 agttcagcat catctttacg tagccctaca ccggtgccat caccgaagta ttttttgtct
    19861 tttactgatg agccagcaaa ggcgaaatct ttaccagcag gttgcttgag gaatccttcg
    19921 ctggcagcaa cttcatcttg taacgcagca tccagacgtc ctgcagccag atcggaatag
    19981 accaaatcct ggttggcata ggccaccaca tccacgcctt tgctacgcca ggtctcgtta
    20041 gcgtaagctt cctgggttga tccctgcagc acaccaacat gtttaccttt cagtgaatcc
    20101 agcgttggct gaatcggtga acctttggca gcaatcaaac gagaatctgc ggcgtacagc
    20161 ttgtcggaga aggcaatctc ctgctgacgt ttatcggtaa tggaaagcga cgaaataatg
    20221 gcgtcgattt ttttcgcttt cagtgagggg atcagcgcgt caaagtcact ggcaacccag
    20281 gtacatttca cctgcatccg tttgcacatc tcgttaccga gatcgatatc aaagccaaca
    20341 aaatcacctt tagcatcttt cgacgagaac ggcgcatagg tggtatcggt tccgatacgt
    20401 accgtctccg gtagcgccgc atagctggaa gccgctgcgg agagaccgac taacaaagac
    20461 agagcgagaa tcgacttctt catacataac cctcaagtga aaatggcttt tttatgttgt
    20521 gtattgtgtt gtgtgtttgc aggctctttc atgcaggtct tatgccatct tgacgacaac
    20581 agtaacattc aacgttaaat atgttaataa gacgttgcat tattgtcctg aagttgaaga
    20641 tagcaggtat agcggttgaa tcgcagcgtt tcgattgaat ggcagaaaca aaatgtcgag
    20701 gatttgatcg cggttgcaaa attgccctga aacagggcaa cagcggagtt atgcgccctg
    20761 ccagcgggca aagagatctt caggaagggt tatcgcaaac tggtcaagaa cacgattaac
    20821 cgtctgattt atcacatcat caagggattg cgggcgatga taaaacgccg gaacgggagg
    20881 cataatcacc gcaccgattt ctgctgcctg agtcattaaa cgcagatggc ctaagtgcaa
    20941 tggtgtttca cgcacgcaga gcaccaacgg gcgacgctct ttcagcacca catctgccgc
    21001 acgggtcagt aaaccatcgg tgtagctatg gacaatgccg gaaagggttt tgattgaaca
    21061 gggtaaaata accatcccca gcgtctggaa agaaccggaa gagatgctgg cggcaatatc
    21121 gcgcgcatcg tgcgtgacat cagctaatgc ctgcacttcg cgcagggaaa aacccgtttc
    21181 gagggataag gtctggcgcg ccgcctggct catcaccaga tgcgtttcga tatctgcgac
    21241 atcgcgcaga acctgtaata agcgcacgcc ataaatcgcg ccgctggcac cgctgatgcc
    21301 tacaatgagt cgtttcataa atagttgccc tgtcagactt gcaggcagac tttgcaggat
    21361 ttcggcggga gttgcaagtc agggtgccag accggcaccc tcagcgaagg catcatcctt
    21421 cgttatgcat ttcgagattt tccacttcgt tctgacgttg cactgctttg gcgtcatcat
    21481 tacgtaacgt atcgaggaaa tcgaggtagc cctgatcaac atctttggtg acgtagacgc
    21541 cgttgaacac cgagcattca aactgctgga tatccggatt ttcagcgcga acggcgtcga
    21601 tcagatcgtt cagatcctgg aaaatcaacc cgtcagcacc gatgatctgg cgaatttcat
    21661 caacttcgcg accgtgagcg ataagctccg tggcgctcgg catatcaata ccataaacgt
    21721 tcgggaagcg aatttccggt gccgcagaag cgaggtacac tttcttcgct ccggcttcgc
    21781 gtgccatctc gataatctgc tcagaagtgg tgccacggac gatggagtcg tcgaccagca
    21841 ggacgttttt atcgcggaac tcggcgcggt tggcattcag tttacggcgc acggacttac
    21901 gacgcagctg ctggcccggc atgataaagg tgcggccaac atagcggttt ttaacgaagc
    21961 cctggcggta cggtttgccc agaatacgag caatttccag cgcgatatca cacgaggttt
    22021 ctgggatcgg gatcaccacg tcgatatcca gatcttccca ttcgcgggca attttctcgc
    22081 ccagtttcgt gcccatattc acacgcgcgc tgtaaacgga aattttgtcg atgaacgagt
    22141 ccgggcgagc aaagtataca tactcaaaca ggcacggatt gctgaccgga ttgtcagcac
    22201 attgacgggt aaacaactgc ccttcttcag taatgtaaat cgcttcgcca ggcgcgacgt
    22261 cacgcaggaa atcaaagccc agcgtatcga gcgctacgct ttcggaagcg accatatatt
    22321 ctgtacggtt ctcgtcaata tcacgttttc ccagtaccag cggacgaatc ccgtttggat
    22381 cgcggaaagc aaccataccg tggccgataa tcatcgccac acaggcatat gcgccgcgga
    22441 ttaagcggtt tgtggcagca atggcagcga aaatattgtc ggcttccagc gggtagtggc
    22501 ggaaattgtc cagctcgctg gcgaagatat taagcagaat ttccgagtcg gaagtggtgt
    22561 tgatgtggcg gcgtttttct tcaaacagtt ttttacgcaa ctcgtgagcg ttggtcagat
    22621 tgccgttgtg ggcaagcgta atgccatacg gggagttaac gtaaaacggc tgcgcttcag
    22681 aggcgctgga gctgccagcc gtagggtaac gcacatgacc aatgcccata ttgccctgca
    22741 aacgctgcat atggcgagct tcaaatacat cgctcaccag cccgttcgct ttacgcaaac
    22801 ggaagcagtt attggcatct atggtgatga tgccggcggc atcctgaccg cgatgctgaa
    22861 gcaccgttaa ggcatcataa atcgactggt taaccggcat aacaccggcg ataccgacaa
    22921 taccgcacat acgtcttttt cctcgttaag ccacatctca gagcacttac gctctgggca
    22981 agaaacttga cgagctttgc agataatcaa aaaaccatct gatgataaaa ctgaactgcg
    23041 ggatcagctg tgatttgctc cagtcttcgc ttttcgacac cccggtaaag gagtcgagaa
    23101 agaagagaat ggcggcaaca atcaacacac cgcgcaacgc accgaaacag acgcccagca
    23161 cccgatcggt gcctgacaac cccgttttct ccaccagctg gcctatcacg aagttcacga
    23221 tagcaccaac gatcagggta gcgataaaca gtaccgcgat ggcaattcca tttcgaacca
    23281 gttcgtcttc aaagcccgta aaccagactg acaggtaagt gtagtaatga ctggcaacaa
    23341 agaaagcaca accccatgtc accagcgata acgcttcacg aacaaagccg cggatcaggc
    23401 taaccagaga ggaaaaagca atcaccgcga ttatggcgta atcaatccag accatatgtg
    23461 tcccacgatt ttacgccctg tcatcctgtt cggggcgcat tctaacagaa aaagaaaacg
    23521 tttgcgtagg gatttccttc ccgcgcatca ataaaaatgg cgctgaaaaa atattcaacg
    23581 ccatcgactt tttatgcctt tgcggcatcg tgcaatgcgt gtcggatgcg gcgtaaacgc
    23641 cttatccgac ctacggttct aacactgccc aggcctgata agacgcgcca gcgtcgcatc
    23701 aggcaagact gtattaattc ggcgtatagc ccattaccac gccacttaag ccagaaagtt
    23761 gcttcaactc acccagcgaa cctttcagct tatctttcga ggcatccggc ccaaccagaa
    23821 tacgggtaat tttaccctgc actggcgtgg atggcgacgt ataaacccga taaccggcac
    23881 cgcgcagctt accgacaatc tcattcactt tatcggcatt tttcagcgca cccagttgca
    23941 caacataggc tttacccgtt ggcgctgctt tttcttccac gaccggcttc ggttctggct
    24001 tcggcgcagg tggtgcttca accttcggtt taggcggctc caccggtttc ggcttcggtg
    24061 gggcgaccgg tgcaggttcc ggttcaaact cggtgttatt ggctgcaata gtggtcggat
    24121 cgagcgacgg tgctgcggca tcacctgccc gcacctcttc cgctgcgcct tccggcggct
    24181 gcgtcggcaa cgcctgggtg gcggctggca tcatatcagg ctcatcgcga tcgcccgctt
    24241 tcggcaccag cgggatagcc gcgaactcat cctgataatg ttttttctgc ccgtccagca
    24301 gccctggaag tacaatcacc cccagcgcca ccagcacgat cgtgcccact aaccgattct
    24361 gaaacttact tgccaccgct tctcctcgcg tcaatcactt ccatgacatg tgcgacagtg
    24421 tggaaagaac cacacaccag cacggtgtct tccgctttag cgtccgccat tgcggcatcc
    24481 catgcctgcg caacgctatc aaatgatttg ccgttaccca aatgctcaag cagttgttct
    24541 gccgtggcac cgcgcggccc ttccagtggc gcacaatacc agtcatcaac cacgcttttc
    24601 aaccaggcca gagttccggc aatatcttta tcatgtagca taccgataac cgccagcacg
    24661 cgcccgtttt tcggtagcgc tttcatacgc ccggtgagat attccgccgc atgtggatta
    24721 tgcgcgacat caaaaataac gcgtggcgac tcgctcacaa tctggaaacg tcccggcaaa
    24781 attgcgctgg caatcccgtc gcgaatggca ttttcactga cttccagccc gctggcacgc
    24841 agtgccgcca gcgccgttgc ggcattcggt tgcgggacaa gcggcaacgg cagattttcc
    24901 agcgtgccgt gagcatcgct aaacgcccag tcatgatcgg tgacggaata gttccactca
    24961 acgccccgac gttgtaacag tgcacctttt tcctgtgcca catcagcaat ggtagaaggc
    25021 atttccggct caccgacaat tgccggtttt tcgctgcgga agatgcctgc tttctcgcga
    25081 ccaatacttt cgcgatctgg acccagccag tcggtatgat ccagcgcaat actggttact
    25141 accgcgacat cggcatcgac aatattggtt gcgtccagac gaccgcccag ccctacttcc
    25201 agaatcacca cgtcaagttg tgcctgcttg aacagccaca acgccgacag cgtaccgtac
    25261 tcgaaatagg tcagggaaat atcaccgcgt gccgattcaa tctccgcaaa agaggcggtg
    25321 tgggccgatt ccggcaattc ctggccctgc acacgtacgc gctcggtata acgcaccaga
    25381 tgcggtgaac tgtagacgcc cactttgtac cctgccgcca tcagaatcga ctccagcgta
    25441 cggcaggtgg tgcctttgcc attcgtaccc gcaacggtaa acacaaatgg cgctggtttc
    25501 agaacgccaa gacgcgccgc gacctggctc acgcgctcaa ggccgagatc gatagtttta
    25561 ctgtgcaggt tttccagata agaaagccac gaagccagag gcgacgcggc ttgaggagtg
    25621 cgtttgataa tcatggtatc cgctgattcg ttacggtgag aatagcaaaa gggcagagcc
    25681 agtggccctg cccttatcag ttatcaggcc tcaggttcct gatccggtac cgggggtacc
    25741 actacgcctt cacgcggcgc ttcaggattc ggcgctggca gattcatcaa cttcgccaga
    25801 atgctcgcca gtttcaggcg catttccgga cgacggacga tcatgtcgat ggcgcctttc
    25861 tcgatcagga attcactgcg ctggaatcct ggcggcagtt tttcgcgaac ggtctgttcg
    25921 ataacacgcg gaccggcaaa gccaattaac gctttcggtt cagcgatgtt gagatcgccc
    25981 agcatggcga aacttgcaga aacaccgccc atcgtcgggt cggtcagcac ggagatgtac
    26041 ggcaagccgc gctcctgcat ttttgccagt gccgcagagg ttttcgccat ctgcatcagc
    26101 gacatcagtg cttcctgcat acgtgcgcca ccagaggcgg agaagcagat cagcgggcag
    26161 ttatcttcca gcgcctgctc aacggcacgc acgaaacgtg cacccacaac agaccccatt
    26221 gaaccgccca taaaggcgaa ctcgaatgcc gcagcgacaa ccggcattcc atacagagtg
    26281 cctttcatca ccaccagcgc atctttttcg ccagtttctt tctgcgcaga tgccagacgg
    26341 tctttatact tcttggagtc acgaaacttc agcacatctt tcggctcaag ctcgctaccc
    26401 agctccacaa ggcttccttc atctaacagg ctatgcaggc gattacgcgc tgtcatacgc
    26461 atgtgatggt cacacttcgg acagacctca agattacgtt ccagctcagc gcggtataaa
    26521 acctgaccgc agctatcaca cttagtccac accccttcag gaatgctcgc cttgcgggtg
    26581 ggagtaatgt tgcttttaat tcgttcaatc cagctcatta gggacctttc tgtctgaacc
    26641 tggttcgatg ccagttttat ctttggggac gcataatgcc atttttgccc ccaacagacc
    26701 atgaatgttg cacattaaaa cataacagcc cgaaactttg gataaaaaag tggtcgaacc
    26761 gcggagttac tttttatttt gcggcgcgtg ccgcagcgcg tttgtgacgg attatttcga
    26821 tgacgcccgg caaaatagaa accacaataa tcccgacgat cagcagctta aggttatcct
    26881 gaaccatcgg gattgtaccg aagaaatagc ctgcgtaggt aaaaagcagt acccacaaca
    26941 gtgcgccgat cacgttataa gcggcgaaat gacggtacga catgtggccc attcccgcaa
    27001 caaacggggc gaacgttctg acgatcggca caaaacgggc gagaataatc gttttgccgc
    27061 catgtttctc ataaaactga tgggttttgt cgagataact gcgacggaaa attttcgaat
    27121 tgggattact gaacaacttt tcaccgaaca accgcccgat agtgtaattg accgcgtcac
    27181 ccacaatcgc ggcgatcagc atcagcacca ccatcatatg gacgttgaga tcgttggttt
    27241 ccagtgatgc caacgctccg gcaacaaaca gcaatgaatc acccggtaaa aacggcgtta
    27301 ccaccagacc ggtttcacag aacaaaatta agaacaaaat ggcataaacc cagacgccgt
    27361 actccgcgac cagctctgcc agatgcacgt caatgtgcag gataaaatcg atgaggaaat
    27421 aaatcaggtc catatttgcc tatgccttgt actcgtcatc tttcaggctg taactacgtt
    27481 ggctacagcc tgaaatgttc cgagcattat tctcgttagt ccgccagaaa tagcgggccc
    27541 attggcggtt ttggaagatc ataccggtca gggtaatcca ccgcgaccag atacagccct
    27601 tccgcttttg ccgttgctgc cgccagcgtt ctgtcctttg ccgccagcaa ctctgctatc
    27661 cagctctccg gctggttgtg ggcaccgact tccatcaggc tgccgacaat attcctgacc
    27721 atatgatgta caaaagcatt cgctttaata tctaccacca cataaggacc gtgacgcgtg
    27781 acattaatat gcataacgtt gcgccacggg gttcgggact ggcactgcac cgcacggaac
    27841 gaggtgaaat cattctcgcc cagcaagcat tgcgcagccc gatgcatccg ttcagcgtcc
    27901 agcggttcgt aaaaatgggt taccccttta ctcagtaccg ccgggcgcag ccgatgattg
    27961 tagatgatgt agcgataacg gcgagccgtg gcgctaaatc gggcatgaaa atcatcaggt
    28021 acagctttaa cccaacgcac ggcgatgtca ccaggtaaat tcgcatttac gcccaaggtc
    28081 cacgccgcgt ctttgcgctg cgcggttgtt tcgaaatgca ccacttgccc ggtaccgtgt
    28141 acccctgcgt cagtacgccc ggcgcagaag acggtgatgg gttcgttcgc cacctgggag
    28201 agcgcctttt ccagcttctc ctgcacactg cggacttcat tctgccgttg ccagccgtaa
    28261 tacttactgc cgtcgtactc aatgcccagc gcaattttat aaactggcag ttgttgctgg
    28321 tcggacatta gtacagatac tcctgcacca gtttctcggc gatttttact gccatcagcg
    28381 cgccgccaaa gcgaacgtta tcggccaccg accagaactg gacttgctcc ggcataccgt
    28441 agtcattacg cacgcagcca acagaaagat gcggcgtacc cgaagcatcc cctacctgag
    28501 tcgggaattc gttctcttca gagagcacaa tatcttcgcc ctgagcaaac gcatcacgcg
    28561 cttcttctgc cgccagcgga cgcagtgctt caaagttgac catctgggca tgaccgtaga
    28621 ataccggtgc ctggacgacg cttgccgaaa tcatcagccc ttcgtcctgc aggattttgc
    28681 gtacttcgtc aacgatacga cgttcttcac gcacgctacc ttcgctatcc ggcaacaacg
    28741 gcagcatatt gaacgccagt tgacgaccga agaaatcttc ttcgtcaatc ggaatgccgt
    28801 tgagcaattt cgcactctgc cccgctaacg catcgaccgc ttttttgccc tgggcggagg
    28861 ctgaaatcag gctggtaaca ctgatacgcg acaaaccgcc ctgatcgatt aacggtttca
    28921 gtgccgccag cagctggctg gtcagactgt ctggtacggc gatgacattc cggttgcggt
    28981 aatcggtcag tacaaacggg tttacttccg gcaccaccag cggtacgtcg ggttcgagag
    29041 caaacagtcc gctgctgtcg atcaccaggc aacctgagtt ggtcgcttct tcaacccagg
    29101 cagcggtagc ttctttgcct gcgacaaaaa atgccagctg cgcctgcgtc cagtcgaatt
    29161 cagcggcatc ctgcacggtg attgttttac caccaaagcg cagttgttcg cctgcgcttt
    29221 cgttacgtgc cagtgcataa atttccccaa ccgggaactg acgttcagcc agcgtttcaa
    29281 gcagggcttc gcccacagcg ccagttgcgc ccaggacggc aatgttccag ccttcagaca
    29341 tggtggttta ctccagaaat agcaaagctc cctgccaatg ttacagcaga gagcatgaag
    29401 aagagattaa cgtgccggat gatgaacggc gttaaaaccc agtttacaca gcaatgatgc
    29461 cgcactggcg tcatcacaaa ttacatacag agatgaccat tcacggcgct caagatagtt
    29521 tttgcgcagt ttatcgaact cacccggtat cccggcgact ttacgcagcg gtgcgtcatc
    29581 gcggcgcaca tcatacacca aatgcaccag ccttttcagc gtcggttgat cgagcgggcc
    29641 atgcagcgta atgcgaccaa actctggcgc gggcagtaat gtatccagcg caacgtgctg
    29701 ttcatgccca ataaacttgc tataggcttc aaacacttgc gtagtaccgc gtgctttacc
    29761 ttccagggta taacctgcga tatgcggcgt gccgatatcc acttttgtca gcaactccac
    29821 gttaagttcc ggttcgcctt cccagacatc cagcactacg cttaacttct ggccttcatt
    29881 caggcaggtc agcaacgcag tattatcgac gactgcgcca cggcaggcgt taatcagaat
    29941 cgctcccggc ttcaggctac ggatcagttt ttcatccgcc agatgcagcg ttttgtacgg
    30001 cccatctttg aagagtggcg tatggaaagt cagaatatcc gcatgctgaa ctaattcatc
    30061 cagcgagcgg aaatcaccct catccccgcg gtcagcgcga ggcggatcgc aaagcaatgt
    30121 tttaatccct aacgcttcca gtcgcgcctg caatcgacgt ccaacgttac caacgcccac
    30181 gatccccacc gtacggtcgt gcagtgaaaa tccatcgcgt tcggcaagca tcagtagtga
    30241 ggagaaaaca tattccacca ccgcaatcgc attacagcca ggtgcagcgg aaaaaccaat
    30301 tcccgcctgc ttcaaccatg cttcatcgac atggtcggtc cccgcagtgg cagtgccaac
    30361 aaatttaatg ggttttcctg ccagcaaaga ttcattcact ttcgtgaccg aacgcaccat
    30421 cagcgcatcc gcgtctgcca gttgagcaac ggggattgga cgcccgggaa ccgcggtcac
    30481 ctcacccaaa cggctaaata agtcgcgggc ataaggcata ttttcatcaa caaggatttt
    30541 cacgtttgtg ttacctgtat gagacgagag ttaaccggac aagtgtgcca taatctcgcg
    30601 gccaggcata cttgcgaaga tttcaggtat aaggatacgt aatgatacaa cctatttccg
    30661 gccctcctcc tgggcaacca ccaggtcagg gagataacct gccgtctggc gcgggcaatc
    30721 agcctttatc cagtcagcaa cgtacttcgc tggaaagctt aatgacgaaa gtgacctcac
    30781 tgacgcaaca acaaagagca gaactgtggg cgggtatcag gcacgatatt ggtctgtcgg
    30841 gagattcacc gctgctttcg cgtcacttcc ctgccgctga gcataatctg gcgcaacgtc
    30901 tgctggccgc gcaaaaaagc cattctgccc gccagctttt agcgcaatta ggggagtatt
    30961 tacgtctggg gaataatcgt caggcggtca cggattatat ccgtcataac tttggtcaga
    31021 cgccgctgaa tcagctctca ccggagcaat taaaaaccat tctcaccctg ttgcaggaag
    31081 ggaagatggt tattccgcaa ccacagcagc gcgaggcgac cgaccgtcct ttattaccgg
    31141 cggagcacaa tgcgctaaaa cagctggtga ccaaacttgc ggcggcaacg ggggaaccca
    31201 gcaaacagat ctggcaatcg atgctggaac tttccggggt gaaagatggc gagttaattc
    31261 cagcgaaact gtttaaccat ctggtgacct ggttgcaggc gcgccagacg ttaagccagc
    31321 aaaatacgcc gacgctggaa tcactacaga tggcgctaaa acaaccttta gatgccagtg
    31381 aactggcggc gttatcggca tatatccagc aaaaatatgg cctttctgcg caatcatcgc
    31441 tttcttctgc ccaggccgag gatattctta atcagcttta tcaacggcgg gttaaaggga
    31501 ttgatccgcg tgatatgcaa ccgctgctta atccttttcc accgatgatg gacacgttgc
    31561 aaaatatggc aacgcgtccc gcgctgtgga tactgttagt cgcgattatc ctgatgctgg
    31621 tctggctggt tcgttagccc cgacgaaatg acagtatcgt gacaataatt cccagcaccg
    31681 cagagatcgc cccggcaaga aataccgaag agtaaccaaa cgtggtcgcc agcattcccg
    31741 ccagcggccc ggagacgccg agggcgatat cctgaaacgc ggcgtaaccg cccagtgcgg
    31801 tgccgcgaac ttgtgagggg acgcgtttaa ccacctccac gcccagcgca ggaaagataa
    31861 gcgaacatcc ggctccggtt aacgccgcgc ccgctaatgc gacccacgca cctggggctt
    31921 gccagagcag caacaagccc accgtttcta caagcagaga gacaatcgcc actttcacgc
    31981 cgccaaaacg gtccggcatc cagccaaaca tgacgcgcat cacgacaaat gcgccgccaa
    32041 acgcggtaag agtaaagccc gccatcgccc atcctttgct ggcaaagtag agcgaaacga
    32101 aagtcccgat aaccgcaaaa ccaacgcctt gtagtgccag acctaaccct ggtttccaga
    32161 taagcccgac aacgctccac agcgatggac gttcttccgc cagggccggt actttgcgca
    32221 ctgtgccgtt acaggcccac gccagtacgg gtaataccat tgtggtgatc gccagtgcgg
    32281 caaaaccgta atggctatga atcaacaggc caagcggagc accaacagcg agggcaccgt
    32341 aaatcgccat tccgttccat gacatcactt tgccagagtg ttttggccct acgatgccta
    32401 gtccccaggt cagagcgcct gtcagtaact ggctttcacc aaagccaaga atcaaacgcc
    32461 cgacgaccaa cagagcaaat ttgaacggtg cggagacagg caaaatcgcc gccagcagca
    32521 acgcgccgcc agccagacca caagctaaca ttccctgaag cgccgaacgt tttgcaccat
    32581 attgatcggc cagtcgcccg gcgtaaccac gcgtcagcac cgtagccaga aactgaatcc
    32641 cgacggcaat gccgaccatg gtattgccat agcccagttc atgatgaaca aacagcggga
    32701 taaccggcaa cggcaaccct acggtcatgt aggtgagaaa aaccgcaaaa gcgatgcgga
    32761 agagcgaaaa attggcagaa gatcgtgttt cggtttggct tacagcagtc atgcattact
    32821 ccagaatgca gcgcaaggcg aggagtatcc ccgtctcatc tctctggttt cagggttacg
    32881 gtgcgttggc aggatttaac gcgtacgtct tttcagaagg aaatcgacaa agcgggaagt
    32941 ttgcctggaa ctggcggcga ttgtcaatga tgtggaaaag ggaaccatca ggttcccttt
    33001 tgcgttagtg ccggaggccg cattggcgcg taacgtcgga tgcgacgctg gcgcgtctta
    33061 tccgacctac tccgactact ccgactactc cgacctactg cgaattaatc tttcagcttg
    33121 cgcattacca gcgtggcgtt ggtgccgccg aagccgaagc tgttagacat aacggtggtc
    33181 agttcgcgat cggtcgtttc ggtcacgatg ttcagacctg cagcctgctc gtccagctct
    33241 tcaatgttga tgctcggggc gataaagccg tgttccagca tcagcagaga gtagatagct
    33301 tcctgtacgc cagcagcgcc cagagagtga ccggtcatgg ctttggttgc agaaatcgcc
    33361 gggctcttat cgccgaacac ttcacggata gctgccagtt ctttcacgtc gccaaccgga
    33421 gtcgaagtac catgagagtt caggtaatcg attggggtat caacgccgtg catcgccatc
    33481 ttcatgcagc gtactgcgcc ttcgccagac ggagcaacca tgtctgcacc atcagaggtt
    33541 gcgccgtagc caacgatttc agcatagatg tgagcaccac gcgccagcgc gtgttccagc
    33601 tcttcaacca ctaccatacc gccgccgcca gcgataacga aaccgtcacg gtgagcgtcg
    33661 taagtacggg aggctttttc cggggtgtcg ttgtatttag tagacagcgc acccatcgcg
    33721 tcgaattcgc aggccatttc ccagcacagc tcttcgccgc cgccagcaaa cacgatgtcc
    33781 tgtttgccca gttggatctg ctctactgcg ttaccgatac agtgtgcgga agtcgcacac
    33841 gcggagctga tggagtagtt aacgccatga attttaaacg gcgtggcgag gcaggcagaa
    33901 acgccggatg ccatcgcttt ggtgaccaca tacgggccaa ccgctttcag gccgcgcggg
    33961 ccgcgcattg cgtcagcgcc gaacacctgg aaacgcgggg aaccgccgcc agaacctgca
    34021 atcaggccaa cgcgcgggtt attctggtaa gcttccggag aaaggcccgc atctgcgatt
    34081 gcctgctcca tagaaaggaa tgcataaatg gatgcgtcgc tcataaagcg cacaactttg
    34141 cggtcaatga ggccagtggt atccagtttt acgttgcccc agacgtggct acgcatgccg
    34201 gaatccttca gctcctgaga gaaagtgatc cctgaacgtc cttcacgcag agatgccagg
    34261 acttcctgct ggttattacc gatgctggaa acaatgccca ggccagtaat cactgcacgt
    34321 ttcattcaat acctctgtaa gtcgcacata gagtaagttt cgaatgcaca atagcgtaca
    34381 cttgtacgcc gaacaagtcc gatcagccat ttaatagaga aatttgcgca gccttacaca
    34441 catcgctaag atcgagccac cgcctgtaag acgagtaact tacgtgaaac actactccat
    34501 acaacctgcc aacctcgaat ttaatgctga gggtacacct gtttcccgag attttgacga
    34561 tgtctatttt tccaacgata acgggctgga agagacgcgt tatgtttttc tgggaggcaa
    34621 ccaattagag gtacgctttc ctgagcatcc acatcctctg tttgtggtag cagagagcgg
    34681 cttcggcacc ggattaaact tcctgacgct atggcaggca tttgatcagt ttcgcgaagc
    34741 gcatccgcaa gcgcaattac aacgcttaca tttcattagt tttgagaaat ttcccctcac
    34801 ccgtgcggat ttagccttag cgcatcaaca ctggccggaa ctggctccgt gggcagaaca
    34861 acttcaggcg cagtggccaa tgcccttgcc cggttgccat cgtttattgc tcgatgaagg
    34921 ccgcgtgacg ctggatttat ggtttggcga tattaacgaa ctgaccagcc aactggacga
    34981 ttcgctaaat caaaaagtag atgcctggtt tctggacggc tttgcgccag cgaaaaaccc
    35041 ggatatgtgg acgcaaaatc tgtttaacgc catggcaagg ttggcgcgtc cgggcggcac
    35101 gctggcgaca tttacgtctg ccggttttgt ccgccgcggt ttgcaggacg ccggattcac
    35161 gatgcaaaaa cgtaagggct ttgggcgcaa acgggaaatg ctttgcgggg tgatggaaca
    35221 gacattaccg ctcccctgct ccgcgccgtg gtttaaccgc acgggcagca gcaaacggga
    35281 agcggcgatt atcggcggtg gtattgccag cgcgttgttg tcgctggcgc tattacggcg
    35341 cggctggcag gtaacgcttt attgcgcgga tgaggccccc gcactgggtg cttccggcaa
    35401 tcgccagggg gcgctgtatc cgttattaag caaacacgat gaggcgctaa accgcttttt
    35461 ctctaatgcg tttacttttg ctcgtcggtt ttacgaccaa ttacccgtta aatttgatca
    35521 tgactggtgc ggcgtcacgc agttaggctg gaatgagaaa agccagcata aaatcgcaca
    35581 gatgttgtca atggatttac ccgcagaact ggctgtagcc gttgaggcaa atgcggttga
    35641 acaaattacg ggcgttgcga caaattgcag cggcattact tatccgcaag gtggttggct
    35701 gtgcccagca gaactgaccc gtaatgtgct ggaactggcg caacagcagg gtttgcagat
    35761 ttattatcaa tatcagttac agaatttatc ccgtaaggat gactgttggt tgttgaattt
    35821 tgcaggagat cagcaagcaa cacacagcgt agtggtactg gcgaacgggc atcaaatcag
    35881 ccgattcagc catacgtcga ctctcccggt gtattcggtt gccgggcagg tcagccatat
    35941 tccgacaacg ccggaattgg cagagctgaa gcaggtgctg tgctatgacg gttatctcac
    36001 gccacaaaat ccggcgaatc aacatcattg tattggtgcc agttatcatc gcggcagcga
    36061 agatacggcg tacagtgagg acgatcagca gcagaatcgc cagcggttga ttgattgttt
    36121 cccgcaggca cagtgggcaa aagaggttga tgtcagtgat aaagaggcgc gctgcggtgt
    36181 gcgttgtgcc acccgcgatc atctgccaat ggtaggcaat gttcccgatt atgaggcaac
    36241 actcgtggaa tatgcgtcgt tggcggagca gaaagatgag gcggtaagcg cgccggtttt
    36301 tgacgatctc tttatgtttg cggctttagg ttctcgcggt ttgtgttctg ccccgctgtg
    36361 tgccgagatt ctggcggcgc agatgagcga cgaaccgatt ccgatggatg ccagtacgct
    36421 ggcggcgtta aacccgaatc ggttatgggt gcggaaattg ttgaagggta aagcggttaa
    36481 ggcggggtaa tctgctctcg cagtgtttgc cggatgcggc gtgaacgcct tatccgacct
    36541 acgtgtgacg
//
LOCUS       DAPHDW010000033        35891 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.033, whole genome shotgun sequence.
ACCESSION   DAPHDW010000033 DAPHDW010000000
VERSION     DAPHDW010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35891)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35891)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35891
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.033"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(192..332)
                     /locus_tag="RH057_001622"
     CDS             complement(192..332)
                     /locus_tag="RH057_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128009.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            428..1144
                     /gene="arcA"
                     /locus_tag="RH057_001623"
     CDS             428..1144
                     /gene="arcA"
                     /locus_tag="RH057_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HDV3128010.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(1204..2556)
                     /gene="creD"
                     /locus_tag="RH057_001624"
     CDS             complement(1204..2556)
                     /gene="creD"
                     /locus_tag="RH057_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HDV3128011.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEDDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELDAPNITLGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLKLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREVSESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHTGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTMDDQLRIWK
                     "
     gene            complement(2614..4038)
                     /gene="creC"
                     /locus_tag="RH057_001625"
     CDS             complement(2614..4038)
                     /gene="creC"
                     /locus_tag="RH057_001625"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HDV3128012.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDSQG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMDGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVTPTEVNVAAEPALLDQALGNLLDNAIDFTPESGRITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(4038..4727)
                     /gene="creB"
                     /locus_tag="RH057_001626"
     CDS             complement(4038..4727)
                     /gene="creB"
                     /locus_tag="RH057_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HDV3128013.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QLVPDVIILDVGLPDISGFELCRQLLALHPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSSPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDTHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(4740..5213)
                     /gene="creA"
                     /locus_tag="RH057_001627"
     CDS             complement(4740..5213)
                     /gene="creA"
                     /locus_tag="RH057_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HDV3128014.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            5424..6293
                     /gene="robA"
                     /locus_tag="RH057_001628"
     CDS             5424..6293
                     /gene="robA"
                     /locus_tag="RH057_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HDV3128015.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(6290..6937)
                     /gene="gpmB"
                     /locus_tag="RH057_001629"
     CDS             complement(6290..6937)
                     /gene="gpmB"
                     /locus_tag="RH057_001629"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HDV3128016.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            6989..7510
                     /gene="yjjX"
                     /locus_tag="RH057_001630"
     CDS             6989..7510
                     /gene="yjjX"
                     /locus_tag="RH057_001630"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HDV3128017.1"
                     /translation="MHQVVCATTNPAKIQAILQAFHEIFGEGSCHIASVAVESGVPEQ
                     PFGSEETRAGARNRVANARRLLPEADFWVAIEAGIDGDSTFSWVVIENTSQRGEARSA
                     TLPLPAVILQKVREGEALGPVMSRYTGIDEIGRKEGAIGVFTAWKLTRASVYHQAVIL
                     ALSPFHNAVYQAL"
     gene            complement(7595..7921)
                     /gene="trpR"
                     /locus_tag="RH057_001631"
     CDS             complement(7595..7921)
                     /gene="trpR"
                     /locus_tag="RH057_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HDV3128018.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(8011..9948)
                     /gene="sltY"
                     /locus_tag="RH057_001632"
     CDS             complement(8011..9948)
                     /gene="sltY"
                     /locus_tag="RH057_001632"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HDV3128019.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFVRTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLMQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSALTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            10159..11826
                     /gene="ettA"
                     /locus_tag="RH057_001633"
     CDS             10159..11826
                     /gene="ettA"
                     /locus_tag="RH057_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HDV3128020.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEQPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(12133..13365)
                     /gene="nadR"
                     /locus_tag="RH057_001634"
     CDS             complement(12133..13365)
                     /gene="nadR"
                     /locus_tag="RH057_001634"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HDV3128021.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIDTVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQR"
     gene            complement(13386..14768)
                     /gene="radA"
                     /locus_tag="RH057_001635"
     CDS             complement(13386..14768)
                     /gene="radA"
                     /locus_tag="RH057_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HDV3128022.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSMMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(14817..15785)
                     /gene="serB"
                     /locus_tag="RH057_001636"
     CDS             complement(14817..15785)
                     /gene="serB"
                     /locus_tag="RH057_001636"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HDV3128023.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            15891..16535
                     /locus_tag="RH057_001637"
     CDS             15891..16535
                     /locus_tag="RH057_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HDV3128024.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            16563..17579
                     /gene="lplA"
                     /locus_tag="RH057_001638"
     CDS             16563..17579
                     /gene="lplA"
                     /locus_tag="RH057_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
                     /protein_id="HDV3128025.1"
                     /translation="MSTLRLLISDSYDPWFNLAVEECIFRQMPATQRVLFLWRNADTV
                     VIGRAQNPWKECNTRRMEEDNVRLARRSSGGGAVFHDLGNTCFTFMAGKPEYDKTIST
                     SIVLNALNALGVSAEASGRNDLVVKTAEGDRKVSGSAYRETKDRGFHHGTLLLNADLS
                     RLANYLNPDKKKLAAKGITSVRSRVTNLTELLPGITHEQVCEAITKAFFAHYGERVEA
                     EIISPDKTPDLPNFAETFARQSSWEWNFGQAPAFSHLLDERFSWGGVELHFDVEKGHI
                     TRAQVFTDSLNPAPLEALAGRLQGGLYRADMLQQECEALLVDFPDQEKELRELSTWIA
                     GAVR"
     gene            17611..17760
                     /locus_tag="RH057_001639"
     CDS             17611..17760
                     /locus_tag="RH057_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128026.1"
                     /translation="MIDLENQEREIINIMLSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLK"
     gene            complement(18035..18754)
                     /gene="deoD"
                     /locus_tag="RH057_001640"
     CDS             complement(18035..18754)
                     /gene="deoD"
                     /locus_tag="RH057_001640"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HDV3128027.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGIDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(18834..20057)
                     /gene="deoB"
                     /locus_tag="RH057_001641"
     CDS             complement(18834..20057)
                     /gene="deoB"
                     /locus_tag="RH057_001641"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV3128028.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(20109..21431)
                     /gene="deoA"
                     /locus_tag="RH057_001642"
     CDS             complement(20109..21431)
                     /gene="deoA"
                     /locus_tag="RH057_001642"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HDV3128029.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVIHAKDENSWQEAAKAVKAAIKLADKAPESTPTVYRRISE"
     gene            complement(21558..22337)
                     /gene="deoC"
                     /locus_tag="RH057_001643"
     CDS             complement(21558..22337)
                     /gene="deoC"
                     /locus_tag="RH057_001643"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HDV3128030.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGNEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            22595..24145
                     /locus_tag="RH057_001644"
     CDS             22595..24145
                     /locus_tag="RH057_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HDV3128031.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQDEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAELKQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIAN
                     GPVHDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYC
                     QQQIAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCKKEG
                     IAARYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGAR
                     LPYGDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMRE
                     FTANVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISH
                     EQQMRFSQ"
     gene            24117..24980
                     /locus_tag="RH057_001645"
     CDS             24117..24980
                     /locus_tag="RH057_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HDV3128032.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKRCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSICLLAERGKLAELRLLVIPDQ
                     VDYLHHIDELATFIKRLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(25093..25875)
                     /locus_tag="RH057_001646"
     CDS             complement(25093..25875)
                     /locus_tag="RH057_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3128033.1"
                     /translation="MICRFIDTHCHFDFPPFSGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAEKYQPLYAALGLHPGMLEKHSDVSLDQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLSRTGVVHGFS
                     GSLQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QANRPEQAARVFAVLCELRSEPADEIAEVLLNNTYAVFSVSG"
     gene            complement(25872..26945)
                     /locus_tag="RH057_001647"
     CDS             complement(25872..26945)
                     /locus_tag="RH057_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HDV3128034.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAYICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRIFEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(27067..27228)
                     /locus_tag="RH057_001648"
     CDS             complement(27067..27228)
                     /locus_tag="RH057_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HDV3128035.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(27355..27960)
                     /gene="osmY"
                     /locus_tag="RH057_001649"
     CDS             complement(27355..27960)
                     /gene="osmY"
                     /locus_tag="RH057_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HDV3128036.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRHVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(28353..29942)
                     /gene="prfC"
                     /locus_tag="RH057_001650"
     CDS             complement(28353..29942)
                     /gene="prfC"
                     /locus_tag="RH057_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HDV3128037.1"
                     /translation="MTLSPYLQEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLDGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     SRLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(30033..30710)
                     /gene="yjjG"
                     /locus_tag="RH057_001651"
     CDS             complement(30033..30710)
                     /gene="yjjG"
                     /locus_tag="RH057_001651"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HDV3128038.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRGNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHHREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(30725..31171)
                     /gene="rimI"
                     /locus_tag="RH057_001652"
     CDS             complement(30725..31171)
                     /gene="rimI"
                     /locus_tag="RH057_001652"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HDV3128039.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRVLLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(31140..31553)
                     /gene="holD"
                     /locus_tag="RH057_001653"
     CDS             complement(31140..31553)
                     /gene="holD"
                     /locus_tag="RH057_001653"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HDV3128040.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIAIPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            31656..32687
                     /gene="rsmC"
                     /locus_tag="RH057_001654"
     CDS             31656..32687
                     /gene="rsmC"
                     /locus_tag="RH057_001654"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HDV3128041.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSRQMGDNARFSLVATADDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANCVEGEVFASNVFSEVKGRFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
     gene            32955..33041
                     /locus_tag="RH057_001655"
     tRNA            32955..33041
                     /locus_tag="RH057_001655"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32989..32991,aa:Leu,seq:cag)
     gene            complement(33080..33316)
                     /locus_tag="RH057_001656"
     CDS             complement(33080..33316)
                     /locus_tag="RH057_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HDV3128042.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTS
                     VMLYHKQLRHYVLLPSCLALIAAIMLMIMNLNQG"
     gene            33457..34245
                     /gene="fhuF"
                     /locus_tag="RH057_001657"
     CDS             33457..34245
                     /gene="fhuF"
                     /locus_tag="RH057_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HDV3128043.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRATIAEHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRAACFWVDVCEDKNATPHSPQQRMERL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTTGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGDCTLK"
     gene            complement(34283..34960)
                     /gene="bglJ"
                     /locus_tag="RH057_001658"
     CDS             complement(34283..34960)
                     /gene="bglJ"
                     /locus_tag="RH057_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418786.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HDV3128044.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIADDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAADILLCMRHCEA
                     SNVLHPY"
     gene            complement(34918..35643)
                     /locus_tag="RH057_001659"
     CDS             complement(34918..35643)
                     /locus_tag="RH057_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HDV3128045.1"
                     /translation="MLPGCCKNGIVISKIPVMQAGLKEVMRTHFPEYEIISSASAEDL
                     TLLQLRRSGLVIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAVELLM
                     CPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMNETHDFSYRSVILTLSERK
                     VLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGARELN
                     LPSVYGDAAEWNTAELRREMSHS"
BASE COUNT         8543 a         9781 c         9060 g         8507 t
ORIGIN      
        1 aaataaatct cgacaattgg attcaccacg tttattagtt gtatgatgca actagttgga
       61 ttattaaaat aatgtgacga aagctagcat ttagatacga tgatttcatc aaactgttaa
      121 cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag ttttattggg tgtccggccc
      181 ctcttagcct gttatgttgc tgttaaaatg gttaggatga cagccgtttt tgacactgtc
      241 gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt tgttgacgtt gatggaaagt
      301 gcatcaagaa cgcaattacg tactttagtc atgttacgcc gatcatgtta atttgcagca
      361 tgcatcaggc aggtcaggga cttttgtact tcctgtttcg atttagttgg caatttaggt
      421 agcaaacatg cagaccccgc acattcttat cgttgaagac gagttggtaa cacgcaacac
      481 gttgaaaagt attttcgaag cggaaggcta tgatgttttc gaagcgacag atggcgcgga
      541 aatgcatcag atcctctctg aatatgacat caacctggtg atcatggata tcaatctgcc
      601 gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag caggcgaatg ttgcgttgat
      661 gttcctgact ggccgtgaca acgaagtcga taaaattctc ggcctcgaaa tcggtgcaga
      721 tgactacatc accaaaccgt tcaacccgcg tgaactgacg attcgtgcac gcaacctgct
      781 gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt cgtagcgttg aaagctacaa
      841 gttcaatggt tgggaactgg acatcaacag ccgttcgttg atcggccctg atggcgagca
      901 gtacaagctg ccgcgcagcg agttccgcgc catgcttcac ttctgtgaaa acccaggcaa
      961 aattcagtct cgtgctgaac tgctgaagaa aatgaccggc cgtgagctga aaccacacga
     1021 ccgtactgta gacgtgacga tccgccgtat tcgtaaacat ttcgaatcta cgccggatac
     1081 gccggaaatc atcgccacca tccacggtga aggttatcgc ttctgtggtg atctggaaga
     1141 ttaatcggct ttaccaccgt caaaaaaaac ggcgcttttt agcgccgttt ttatttttca
     1201 accttatttc cagatacgta attgatcgtc cattgtaact tctttactgg ctttcatttt
     1261 cggcagtgaa aacgcatacc agtcgatatt acgggtcaca aacatcatgc cggccagcgc
     1321 caccaccagc acactggttc ccaacaacag cgcgctatcg gcagagttga gcagtcccca
     1381 catcacacca tccagcaaca acagcgcgag ggtaaacaac atgctgttgc gccagccttt
     1441 caataccgcc tgcaaataaa taccgttcat taacgcccca atcagactgg cgattatcca
     1501 tgccacggta aaaccggtat gttcagaaag cgccagcaag agcaaataaa acatcaccaa
     1561 tgaaagcccc accagcaaat attgcattgg gtgtaagcgt tgcgcggtga gcgtttcaaa
     1621 aacaaagaac gccataaaag tcagcgcaat cagcagaatg gcatacttag tcgcccggtc
     1681 agttaattgg tattgatcgg ctggcgtcgt cactgcgacg ctaaacgcgg ggaagttttc
     1741 ccagccggta tcattgcctg aagcaaaacg ctcaccgaga ttatttgcaa accagctgct
     1801 ttgccattgc gcctgaaaac ctgactcgct aacttcccgt ttggctggca aaaaatcacc
     1861 taaaaaactg ggatgcggcc agttgctggt taaggtcatt tcgctattac gcccggcagg
     1921 caccacagaa agatcgccgg taccgcttaa attcagggcc atattcagct tcaggttctg
     1981 cttgcgccag tccccttcgg gtaaagggat atgcacgccc tgcccgcctt gctctaaccc
     2041 ggtaccgggt tcgatggtca gcgctgttcc gttaacttca ggcgctttca ccacaccaat
     2101 accacgcgca tccccgacgc taatcacaat aaatggcttg cctaaggtga tatttggtgc
     2161 gtcgagttcg ctaagacgcg aaacatcgaa atcggctttt agcgttaaat cactgtgcca
     2221 gacctgaccg gtataaatcc ctatcttgcg ttcttcaacg ttctgattgc catcaaccat
     2281 caatgactca ggtaaccaaa aatggataaa acttcgtttc cgctccacgg ttttatcgtc
     2341 ttcctgcacc gtataaagct cggtcacagg aatagcgatg agcggcccaa cgagtttttg
     2401 cggcccgctg gtactttggc gaatcgcatc ttccacatcg ctacggtaat cggcacgttc
     2461 gacaatcacc tgccgaatca gcattatcgg aatcaacaac agcaatactg caccaaacag
     2521 ggtggtcatt ttccagaaca ggggggattt caacatagtc ttctcctttg caatggcagc
     2581 aggatacgaa ggctatgtgg gaagaatttg aagctatgtg aagtgacggt gaagtcgaag
     2641 cgaggccagc acgccacctt cctgcacgtt gcgcagcgtg acttcgccgt taaacaaacg
     2701 ggcgacctca ctgacgaacg ccaaccccag accgctgctt ttttgcccat ttgcacgcgg
     2761 caaagagtaa aagcgttcaa aaatacgtga cagcgcgtag tcaggaatac cactaccggt
     2821 atccagcacc ttaagcgtga cgtgttcctg atccacttcg gcgcttagcg ttatacgacc
     2881 gctctcgggg gtaaaatcga tggcgttatc cagtaaattc cccagcgcct gatccagtaa
     2941 cgccggttca gcagcaacat taacctcggt gggcgtaaca tgtagagtga tgtttttttc
     3001 tgccaactgc acggtgcgcg cttcgctgac gcggcggaat aatgccgcca catcaacagc
     3061 agtcaaaacg acttcctgac gattctccag tcttgcctgg cgtagtaacg tttccaccag
     3121 tgcctgcatt cgcgcatttt gcgtcagaat gttgtcagta aaacgagcca ctacttcagg
     3181 cggcggacct tcgcgtaaaa tttccgccgc gccacgaatc gccgccagtg ggctttttag
     3241 ctcatgggtt aacgcataaa catactgctc aatatagttt ttcccttcca gcttcacgcg
     3301 catactttcc agcgcctgcg cgagtttacg cagctcgcta ctaccgagat cgggaagagg
     3361 aacgggctta ttgtcagtga cggaatcagc atagcgagtg agccgggcaa tggagcggtt
     3421 gatccaccaa accatgcctg cgccaatcac cagtgcgatc cccaacaaaa tggcgctggc
     3481 ccataaaatt cgccgctcgc tgcgtttaat gaccggagcc atcgccgcgt tcggtttgcc
     3541 tacgctcaaa acgccaataa gccgcgagcc gtccataatc ggcgcggcaa catacatcac
     3601 cgaactttcg ggatcggcag gattttgcaa cgtgctgcgc gcaccatact gaccacgcaa
     3661 cgttagccag acgtcattcc agcgcgaata atcctgccca acggctttat ttgccgaatc
     3721 gaacaatact ttaccctgcg aatcggtcat atagacatga tattcattgc gcactttgtt
     3781 aatgccaccg atattggcgc gaaacgggcg atgttgtagc tgattaaacg cctgcgccag
     3841 ttgcccatgc gttgggtccc cagagagcaa atcgggacgc gccagctccg ccagcaacgt
     3901 tgcggtgtcg atcaacgtcc cctccgttgc tcttcgcacg cccggtttaa cttctttgac
     3961 aaaaatagcc agtacgaacc aggccgccac cgccaccagt aaaaaatagc ccagcagcaa
     4021 ccgcatgccg atacgcatta caggcccctc aggctatatc ccatgccgcg atgagtatta
     4081 atcggtgaaa gatcgggatt gatggcgcgc agcttggcac gcaacgtttt aatgtgggta
     4141 tcaacggtgc gatcataggt gtcctgcgca tcttcccaca cgctatccat cagttgctgg
     4201 cgggaccaga cgcggcctgg cgacttgagt aacgtcttca gcaataaaaa ctcataccgc
     4261 gtcagcgtta atggcgtgtc aaaccagctg atctgcgccg cgggttcatt caattcaaaa
     4321 tgaccaatac ggatgacggg agacggcgac gagaacttct tcacccgacg cagtaaggtg
     4381 cgcaccctgg cgcacacttc gcggggtgaa aacggtttag ccacatagtc gtcagcgcca
     4441 atttccagcc caagcaggcg atcgacctct tcacttcggg ccgtcaggaa cagtacaggt
     4501 aacgccggat ggagcgccag taactggcgg cacaattcaa agccgctgat atcaggcaaa
     4561 ccaacgtcga gaattatgac gtcgggaacc agctggcgag ctttatccag caccggcaag
     4621 cctcgctcaa agacctcgac ggcaaaacct tcctgctgca acatgtagac cagcgtgtcg
     4681 gctatccctt gctcatcttc cactaaccag accgtttccc gttgcataaa tcgcctctgt
     4741 tattgccgcc acggcatgac aggaaccgcg ctaatcgcgt ttttcggcga accttctaca
     4801 actttgtcgg agtaagccag ataagcgagc gcgttgcgtt tagcatcata aaagcgcacg
     4861 acctgtaacg acttaaagac cagggacgta cgttttttga atactacctc gccctgagct
     4921 ttgccgtttt taatacgatc cgacagttca atcggcccga cttgctgaca agaaatggcc
     4981 gcatcggagg tatcttccgc caggcccaat ccccctttaa tacccccggt tttcgcccgg
     5041 ctcacataac aggtgacatt tttcacatcg ggatcgtcaa aggcttccac aacaattttg
     5101 tgatccgggc cgatcatttt aaatacggtg tcgaccgaac caatctcttc tgcatgagcc
     5161 aatggcccca gcattatcag gcttaaagac aggatcaaat gcttgtattt catattgtta
     5221 ccatttttat ctattacacg agcaattagt tcgtcacggt aaataacata ttgattaaga
     5281 acaaaatctc aatactttta tttccgtcaa gccctaaaac atactctact aagaaaaaaa
     5341 cactgaatgc taaaacagca aaaaatgcta ttatccaatt acctgatgtc aggtgctcgt
     5401 tgttgaaagg atgaggatat tttatggatc aggccggcat tattcgcgac cttttaatct
     5461 ggctggaagg tcatctggat cagcccctgt cgctcgacaa tgtagcggcg aaagcaggtt
     5521 attccaagtg gcacttacag agaatgttta aagatgtcac tggccatgct attggcgcgt
     5581 atattcgtgc tcgtcgtttg tcgaaatcgg cggtcgcact acgcctgact gcgcgtccga
     5641 ttctggacat cgcgctgcaa taccgcttcg actctcaaca gacatttacc cgcgcattca
     5701 agaagcagtt tgcccagact cctgcacttt accgccgttc tcctgaatgg agcgcctttg
     5761 gtattcgccc gccgctacgt ctgggtgaat tcactatgcc agagcacaaa tttgtcaccc
     5821 tggaagatac gccgctgatt ggtgttaccc agagctactc ctgttcgctg gagcaaatct
     5881 ctgatttccg ccatgaaatg cgttatcagt tctggcacga ttttctcggc aacgcgccga
     5941 ccattccgcc agtgctctac ggcctgaatg aaacgcgtcc gagtcaggat aaagacgacg
     6001 aacaagaagt attctatacc accgcgttag cccaggatca ggcagatggc tatgtactga
     6061 cggggcatcc ggtgatgctg cagggcggcg aatatgtgat gtttacctat gaaggtctgg
     6121 gaaccggcgt gcaggagttt atcctgacgg tatacggaac gtgcatgcca atgctcaatc
     6181 tgacgcgccg taaaggtcag gatattgagc gatactaccc ggcagaagat gccaaagcgg
     6241 gagatcgccc aattaatctg cgctgtgaac tgctgattcc gatccgtcgt taacgctgca
     6301 actcatctaa tgcaggggcg tccagatgtg agatgtcccc tgcggtttca acaacccagc
     6361 cagacgccag ccacaggctt tcctgataat ccacgcgcga aatagaacag ttacgcagac
     6421 gtaagcggcg ctctgcccat gctggtaatc cgagaatcgt actcaccaga catcccagtg
     6481 caataccatg gctcaccagc agcggtcggc ttccctgcgg taagtcccgg caagattcca
     6541 gtgccgcatt gacgcgatcg ctgagctcct gcattgactc gccttcagga atacgcccgt
     6601 caacggtgcc attgaccagc tgccgacgcc agttctcttc ttcttcggtc agagaatcga
     6661 tatgtctttt ttccagcaca cccatgttta attcacgcag gcgagaatca aagatgatgt
     6721 cacagccgca ggcctgggcg atgatttccg ccgtacgccg ggtgcgtcct aaatcgctac
     6781 tgatgatatg cgtaatgcca agctctttgg cacgggttgc cacctgcatc gcctgttgct
     6841 cacctttggc ggtcagcggg ctgtcagact ggccctgaat acgtcgctcg gcgttccact
     6901 gcgtttcacc gtggcggact aggtatacct gtaacatgct ttttttccgt atactgctgt
     6961 aacgttaatt ttctgagtaa tgctgattat gcaccaagtt gtctgtgcga ccaccaatcc
     7021 cgctaaaatt caggccattc tgcaggcatt tcacgagatc ttcggcgaag gatcctgcca
     7081 tattgcatcc gttgccgtcg agagcggtgt accggaacag ccctttggca gtgaggaaac
     7141 gcgcgctggc gcacgaaatc gggtagcaaa tgcccgccgt ttacttccag aggctgattt
     7201 ttgggtggcg attgaagctg gcatcgatgg tgacagcact ttcagttggg tggtgattga
     7261 aaacaccagc cagcgcggcg aggcgcgttc ggctactttg ccgttacctg cggtgattct
     7321 gcagaaagtg cgtgaaggtg aggcgcttgg tcccgtgatg tcgcgttata ccggtattga
     7381 tgagattggt cgtaaggaag gggcgatcgg cgtgtttacc gcctggaaac tcactcgcgc
     7441 cagcgtctat caccaggcgg tgatcctggc gctgagtccg tttcacaatg ccgtgtatca
     7501 ggcattgtag gacggataag gcgttcacgc cgcatccggc attcggtgca cgatgcctga
     7561 tgcgacgctg ccgcgtctta tcaggcctac aaaatcaatc gcttttcagc aacacctctt
     7621 ccagccactg gcgcagctcc acgggcgcgg ctttcaggct gttagatcca cgcgtaatcg
     7681 tcgcgatgcc cgcgccgagt tcatttttta actcacgctg gctcatttcg ccgcgcaaca
     7741 gctcttcgac aatacgcacg cgagtcccca acgcttcgcg ctcatctggc gtcagcatca
     7801 gatttaacaa cggtaaatgg agatcgtttt ggtaggcatt cttaagcagg tcgacaaaac
     7861 gtaaccactc ctggtgacgc tgttctgcca tcgctgctga atagggtgat tgttgggcca
     7921 taatatgtcg ccattgttag cgggggaagc aaaatgcctc ccccggttgt actcgctaaa
     7981 gagtacgata gcatatcata aacgtgcgga tcagtaacga cgtccccatt ccgtggcgct
     8041 catcaacgtc ggtttatccc ccatgaaata gcggtagtaa gcgtcataag ccagcacgtt
     8101 cttcacataa ccgcgcgtct cggagaacgg aatactctcg acaaatgcca cagcgtcgat
     8161 acgcccggcg ctgttgccaa gccaggttcg cacccgccct ggtccggcgt tataagctgc
     8221 tgaggagaaa atacggttat tgccaaactg ctgataaaca tattgcagat aactggtgcc
     8281 aatgttgata ttcgtttccg gatccagcaa ttgcccaggg ctactataac cgggaataga
     8341 gaacatcttc accgtatggg tcgctgtacc aggcataatc tgcatcaggc cgctggcccc
     8401 taccggtgat ttcacttttg gattccaggc gctctcctga cgggcaatcg ccatcgcata
     8461 gctttgcggg atctccttac cgctggtgta gcgtttgaaa agatcgttgt aagccagcgg
     8521 gaatcgctct tccagatgat cccatagctt cccggcgatc gttgcctgaa cgctaagatc
     8581 ccaccattgg ttgttgaaag cataccgcgc cagttgagcc tgctctgttt ttgacttgct
     8641 cttcaccaga ttggcccact cgctacgcgc ggtgttatcg agattccagt acatcaactc
     8701 gcgcacgcgc gccatctccg gcccctgagt cagggcgctg tcaacattct gcggcgcttt
     8761 atcaatcttc agctcatact cttctccgat gcgttgtgct gcaaccatcg ggtagaaacc
     8821 acgctgttgc atgagttgat gcaaaatctc tttcgcttca gcttcacgtc cgcgttccag
     8881 caataaatcc gcctgccagt aacgccattc atctttctct tttgcttcca tcggcagacg
     8941 cgccagccag gtattcaggc cgcgacgatc gccggtgcca agtgccattc gcacacggcg
     9001 ttcaatcaac gacgtggatt gcgagcgcat aatggcgtca tcgcgccatt tcgcctgctc
     9061 gtcggtgaca tcgttgccca tcaaacgcca ggcgacgata tcgcgcagct cctgaatctg
     9121 atcttcatta agctgctgcg cctgggcaag cgacgggatc atcagccgtg cattttcagc
     9181 atcctgccgc gccacactgg caaacgccac cgctgccatt tgacgggtaa aatcggtcgc
     9241 tccggttgta cgcacgaagg tcagtaccgt attagggttg ttcgccagtg aaatgattgc
     9301 cgaggcgata gtctggtaat cggcaggcat ctgccctgcc agcactgtta ccaggccagt
     9361 gttacccgct ttcatcgcca gacggatacg ctctaaatac gccagcggat cttgtttacc
     9421 tgacgcacgc cagacgctaa ataacttgtc acaggcgtta ggctggctct tgccggttag
     9481 ccacagctct ttcgcccctt gccaggcttc ttcactctgc ccggtgttcc atttcgcata
     9541 gtagtaatta cattgcgctt cggtagttcc gggcttttcc gggctaaagg ctaacaagcc
     9601 acgccagtct tcacgccgcg ccagttcatt gacgaaacga gattgcagcg tgcgagcggg
     9661 aggaagcgtg gggttagcgc gaacaaagtt agtgaccgtc accgccggtt gattcatcag
     9721 atcatcggtg atctggcggt attccaggta gggataaagc ggataatcct tcagtccagg
     9781 catcatttgt tcgaccacat ccatttgtcg attatcccag gcctgcttga tttgcgcgta
     9841 acggctacgc tgctcatcca gtgagtcggc tcgcgccacg ctgctgaccg tcagcagaca
     9901 gacaccggca gccaacagcc gccaggtaac ttgtttggct ttttccacaa gcgcatcctc
     9961 taagtgtaaa tacatcaatg cagcgtcatg ccgcgtaata gatttatgct aaccagtcat
    10021 tgccgtttac gccacgttac ggacactttt ttacttttac tgcgaaggtg atcgaccaac
    10081 tgggattagg cggggaaaaa ggctacactt cgcctttgaa aactcatcat cacgataaac
    10141 atagaggcga agtccaacgt ggctcaattc gtttatacca tgcatcgtgt cggcaaagtt
    10201 gttccgccga aacgtcatat tttgaaaaac atctctctga gtttcttccc tggggcaaaa
    10261 attggtgtcc tgggtctgaa cggcgcgggt aagtctaccc tgctgcgcat tatggcgggc
    10321 attgataaag acatcgaagg tgaagcgcgt ccgcagccag acatcaagat tggttacctg
    10381 ccgcaggaac cgcagctgaa cccggaacac accgtgcgtg agtccattga agaagcggtt
    10441 tctgaagtgg ttaacgccct gaaacgcctg gatgaagtgt atgcgctgta cgccgatccg
    10501 gatgccgatt ttgacaagct ggccgctgaa caaggccgtc tggaagagat cattcaggct
    10561 cacgacggtc ataacctgaa cgtacagctg gagcgtgcgg cggatgcgct gcgtctgcca
    10621 gattgggacg cgaaaatcgc taacctctcc ggtggtgagc gtcgtcgcgt ggcgttgtgc
    10681 cgcctgcttc tggaaaaacc agacatgctg ctgctcgacg aaccgaccaa ccacctggat
    10741 gccgaatccg tggcctggct ggaacgcttc ctgcacgact tcgaaggcac cgttgtggcg
    10801 attactcacg accgttactt cctcgataac gttgcaggct ggatcctcga acttgaccgc
    10861 ggtgaaggta ttccgtggga aggcaactac tcctcctggc tggagcagaa agatcagcgc
    10921 ctggcgcagg aagcttcaca agaagcggcg cgtcgtaagt cgatcgagaa agagctggag
    10981 tgggtacgtc agggaactaa aggccgccag tcgaaaggta aagcacgtct ggcacgcttt
    11041 gaagagctga acagcaccga atatcagaaa cgtaacgaaa ccaacgaact gtttattcca
    11101 cctggaccgc gtctgggcga taaagtgctg gaagtcagca acctgcgtaa atcctacggc
    11161 gatcgcctgc tgattgatga cctgagcttc tcgatcccga aaggggcgat cgtcgggatc
    11221 atcggtccga acggcgcggg taaatcgacc ctgttccgta tgatctccgg tcaggaacag
    11281 ccggacagcg gcaccatcac tttaggtgaa acggtgaaac tggcgtcggt tgatcagttc
    11341 cgtgactcaa tggataacag caaaaccgtt tgggaagaag tttccggcgg gctggatatt
    11401 atgaagatcg gcaacaccga gatgccaagc cgcgcctacg ttggccgctt taactttaaa
    11461 ggggttgatc agggtaaacg cgttggtgaa ctttccggtg gtgagcgcgg tcgtctgcat
    11521 ctggcgaagc tgctgcaggt tggcggcaac atgctgctgc tcgacgaacc aaccaacgac
    11581 ctggatatcg aaaccctgcg cgcgctggaa aacgccctgc tggagttccc gggctgtgcg
    11641 atggttatct cgcacgaccg ttggttcctc gaccgtatcg ccacgcacat cctggactac
    11701 caggatgaag gtaaagttga gttcttcgaa ggtaacttta ctgagtacga agagtacaag
    11761 aaacgcacgc tgggcgcaga cgcactggag ccgaagcgta tcaagtacaa gcgtattgcg
    11821 aagtaatgcg taaaatgccg gatgcggcgt gaacgcctta tccggcctac aaaccaagaa
    11881 atttcaatac gttgtgaatc tccaggcctg ataagacgcg gtaagcgtcg catcaggcat
    11941 tgagcaccga atgccggata cggcgtgaac gccttttccg gcctacaaca gccttagtat
    12001 ccgcaaattc gtagcgggtc acccgtgctg attcttttaa accttgttta atctcgacag
    12061 cctcttccag agaggttttc aactcatcaa aaaacttatt ttatacctcg cctttgagcc
    12121 gtttcatcgc ggttatctct gctcccccat catctcccgc accaactcca cgcagcgcag
    12181 gaaacggctg tcataatcgt cctcttcaac ccgcacgaat tcgatattgt tctcttccag
    12241 catctccacc agcaagttct ggaactcttt gcgatccacc gaactgccga ggctgcgtaa
    12301 accatccgcc acccacggcg tgttgttctc cagcaggatc accagatcga aacggtattc
    12361 atcaatcaac gcctgtacga acggatgctc acgcccttcg tactttttgc agaacgcctg
    12421 ggtagtgaca aaatcagtgt caataaacgc cactttattg gcatatttca ctgcaaaatc
    12481 aatgtattgt gcgtggccca gcgcgatttt atcgtaatcg gaatactgca atgcgatctc
    12541 atcaccgccg aggtgtgaaa agacataatc gcgaccatat tcccacgcac tggtggtatt
    12601 gaagatattg gcaagtttgt ttaccagggt ggatttaccg ctcgactcgc caccaaggat
    12661 cgccacggta cgtacaaaga acggcttcac ttccgtagga atatattccc agtagcggaa
    12721 cggattttcg cggatctgcg caccgctgat actcataaag gtacgtttcg gatcgaccag
    12781 caccgtatcg atccccagat gttccatata ctgtggcgca tcggcttctt ccgaggtgta
    12841 gatcaggtcc ggctgaatcc ccttttcagc cataaacttt ttgatgccgt tgctccacac
    12901 atcccagccg tgcggatacg gctccatgcc ctcttcgttg aaagcatgaa tgcgaatatt
    12961 tttctgatat ttaaaagttt gtaataacca gcgcagacga tccggcaccg tgggctgctg
    13021 cgacatggcg ctgtcttcaa acaacgcgcg gtcgcgggta tcgtcaaaac ccataatgat
    13081 atgcagctcg tcaacctggc tacaggcgcg ctggataagg tagatatgtc cggtatgcag
    13141 cgggtagaac ttaccgaata cgacaccaat ggttttcttc tgtcggggaa attcaagccc
    13201 caaaaaacgg tgcaacgcct ccagcttttg cgcgctgggg cttttaattt tggcattcag
    13261 taactggctt aaataccctt tggtcatacc gctggcatca gctacctgct gtagcgtgca
    13321 gccctgttgc ttgatggcag ttttcaggta atcaaatgac gacatatctc cctccgtatc
    13381 tctcattata agtcgtcgaa cacgctaagc gcgtcggaga gttttttaac gccaaaaatc
    13441 tgcatccctt ccggcgcttt tttcggtacg ttagccgccg gaacaatcgc ccggcgaaaa
    13501 ccgtgtttcg ccgcttctga aattcgttcc tgaccgctgg gcaccgggcg gatctcccct
    13561 gccagcccga cttcaccaaa caccaccaga tcctgtggca gcggtctgtc acgcaggctg
    13621 gaaaccatcg ccagcagtaa cgctaaatcg gcactggttt cggttacctt cacgccgccg
    13681 accacgttca caaatacatc ctgatcggcc atttgcagac caccgtgacg gtgcaagaca
    13741 gccagcagga ttgccagacg gttttgttcc agccccactg ccacgcggcg cggattcgcc
    13801 atcatcgagt gatcgaccag cgcctgaatc tccaccagca acggacgcgt tccttcccac
    13861 accaccatca cggagctgcc ggaggtcact tcatctccgc gacttaagaa aattgccgaa
    13921 gggttgctga cttcacgcag cccctgttcg gtcatcgcga agacgcccag ctcattcacc
    13981 gcgccgaagc ggtttttatg gctgcgcaag gtgcgaaaac gggagtcggc atcaccatcc
    14041 agaagcaccg aacagtcgat acagtgttcc agcactttag ggccagccag cgagccatct
    14101 ttggttacgt gcccaaccat gacaatcgcc acaccgcgcg ttttggcgaa gcgcgtcaga
    14161 taagccgccg tttcacgcac ctgcgccacg ctaccaggcg atgattgtac atccgccata
    14221 tgcatcacct ggatcgagtc aattaccatc agtttcggtt gctcttcttc ggcaatcagg
    14281 cagatctgct cgatgctggt ttccgacaac atattgagat tgtcagtcgg caggccaagt
    14341 cgatgagcgc gcattgccac ctgttgcagc gactcttcgc cggtgacata cagcgttttc
    14401 atctgctggg ccagtttgca cagcgtttgc agcaacagcg tggatttccc cgcaccaggg
    14461 ttaccgccaa tcagaatggc acttcctggc accacgccgc cgcctagtac gcggtcgaac
    14521 tctttaaatc cggtggaaaa acgcggcagc tcttcaaggc tgatatcgga gagtttctgg
    14581 acttttgcca ccccggcgct accggcatag ccgctgagac gctcgttacg cgccaccgtt
    14641 ggcgacgcag caagacgcac ctcggtgatg gtgttccagg catgacaggc actgcactgc
    14701 ccctgccagc gcggataatc ggccccgcat tcattacaaa caaaggcgcg ttttggagct
    14761 tttgccacgg tgtacctcgt taatgctgtg ccgcccgcag gatggcgggc gagcaattac
    14821 ttctgattca ggctgcctga gaggatgcag aataccccca tcaggtcagc gtgacggatg
    14881 gtgacttccg ccttttcatt cacttttggc ttggcatggt aggcaatccc cagccctgct
    14941 gctttgatca tcggcaggtc attagctcca tcgccaatcg ccacggtctg cgccagcggg
    15001 atttcatact cctgcgcgag gcgagtcaaa gttttcgctt tgtactgcgc gtctacgatg
    15061 tcgccgatca cattgccggt aaatttaccg tccatgatct ccagttcatt ggctaccacg
    15121 gcggtcaggc gcagcttgtc gcgcagatat tcagcaaaga aagtaaagcc gccggaggca
    15181 atcgccactt tccagcccag cgtttccagc ttgagcacca gttgcgttaa gcctggcatc
    15241 agcggcagat tttcacgcac ctgttgcaga atattggcgt cagcgccttt cagcgtcgcc
    15301 acacggctgc gcaggctggc ggtaaaatcg agttcgccgc gcatcgcccg ttcggttact
    15361 tccgccacca tctcgcccgt tccggccagt ttggcaattt catcaataca ttcaatctgg
    15421 atggcggtgg aatccatgtc catcaccagc aaacccggcg tgcgcaggtg cgggattttc
    15481 cccagcgggg cgacatccag ttgcgcttca tgggccaggc gcgtagcccg tgcggtgagt
    15541 gaacctgcca gacgaatcac ctgataatct tccacgcacc aggcggcaac aatcaccatc
    15601 gccgcaccca gtttgctctg gtattgggtc agacgctgtt tatccagccc acgaccatac
    15661 agcagccagc cgctacgacc tgcgtggtaa tccagtggca tcacttcatc accacttaat
    15721 gaaagaggca gacccggcca taaagagaca tcttcaggca ggtcgcacca ggtaatgtta
    15781 ggcattaagg ctcctgtaaa atcgttcgaa gcagggaaaa taacgcatga ggctaccttg
    15841 tatccattgc ttctggcaac attaagtctc aaattttcaa agggtggaag atggctcgca
    15901 caaaactgaa attccggctg catcgggcag tgattgtcct gttctgtctt gccttgttag
    15961 tggcactgat gcagggggcg tcatggttta gtcaaaacca ccagcgacag cgtaatccac
    16021 agctggaaga actggcccgc accctggcgc gtcaggtgac gctgaacgtt gcaccgctga
    16081 tgcgtaccga ctcaccagat gaaaaacgca ttcaggcgat cctcgatcag ttaacggatg
    16141 aaagccgcat cctcgacgcg ggtgtgtatg acgaacaagg cgatcttatc gcccgttcag
    16201 gcgaaagcgt cgaagtgcgc gaccggctgg cgctcgacgg taaaaaagcg ggcggctatt
    16261 ttaaccagca gattgtcgaa ccaattgccg ggaaaaacgg accgctcggc tatctgcgcc
    16321 tgacgctcga cacccatacg ctcgccaccg aagcccaaca ggtggataac accactaaca
    16381 ttttacgcct gatgttgctg ctttcactgg caatcggcgt agtgctgacc cgcacgctgc
    16441 tacagggtaa acgtacccgc tggcagcaat cgcccttcct gttaaccgcc agcaaaccgg
    16501 tgccggaaga ggaagaaagc gagaaaaaag agtgacccat tactacaaga aaggaaatcg
    16561 ttatgtccac attacgcctg ctcatctctg actcttacga cccgtggttt aacctggcgg
    16621 tggaggagtg tatttttcgc cagatgcccg ccacgcagcg cgttctgttt ctctggcgca
    16681 atgccgacac ggtagtgatt ggtcgcgcgc agaacccgtg gaaagagtgt aatacccggc
    16741 ggatggaaga agataacgtc cgcctggcac ggcgcagtag cggtggcggc gcggtgttcc
    16801 acgatctcgg caatacctgc tttaccttta tggctggcaa gccggagtac gataaaacca
    16861 tctccacgtc gattgtgctc aatgcgctga acgcgcttgg cgtcagcgcc gaagcgtccg
    16921 ggcgtaacga tctggtggtg aaaaccgccg aaggtgaccg caaagtctca ggatcggcct
    16981 atcgcgaaac caaagatcgt ggcttccacc acggcacttt gctgctcaat gccgacctta
    17041 gccgcctggc aaactatctc aatccggata aaaagaaact ggcggcgaaa ggcattacct
    17101 cagtgcgttc ccgcgtgacc aacctcaccg agctgctgcc ggggatcacc catgagcagg
    17161 tttgcgaggc cataaccaag gcctttttcg cccattatgg cgagcgtgta gaagcggaaa
    17221 tcatctcccc ggacaaaacg ccagacttgc caaacttcgc cgaaaccttt gcccgtcaga
    17281 gtagctggga atggaacttc ggtcaggctc ctgcattctc gcatctgctg gatgaacgct
    17341 ttagctgggg cggcgtggaa ctgcatttcg acgttgaaaa aggccatatc acccgcgccc
    17401 aggtgtttac cgacagcctc aaccccgcgc cgctggaagc cctcgccggg cgactgcaag
    17461 gcggcctgta ccgcgcggat atgctgcaac aagagtgcga agcgctgttg gttgacttcc
    17521 cggaccagga aaaagagcta cgggagttgt cgacgtggat agcgggggcg gtaaggtaac
    17581 tcgctgatta aaataccaaa ggaagggaat atgatcgatc tggaaaacca ggagcgggag
    17641 attatcaata taatgttaag ccagcgtatt tcctggctag ccgccgtgcg tatcaggcat
    17701 aaattatcgc ttgctgaagt aagtaaaatg ttaggtatct cgattaattc tctaaaatag
    17761 atcgaaaaga cggaacggtt tagctcaaac atcaaaagca aaatggcaga aatttatggt
    17821 tgtccaccag agttgctgat atgcccttcc tggatgacgg ctgaacataa atgaatagat
    17881 tcgtgaatag atacttgaat agattttatt ttctatccat atgaaaaata atagtaaatc
    17941 tataaatcag ttgctattga tgaatagata ctaaaaagcc ggagcagtct cccactccgg
    18001 cttcacacga caatcgcctt tcagcgaaac acaattactc tttatcgccc agcagaacgg
    18061 attccagtgc gattttgatc atgtcgttga aggtggtctg acgctcagcg gcagtggtct
    18121 gctcgtgagt gcggatgtgg tcagacacgg tacagatggt cagggctttc gcgccaaatt
    18181 ctgcagcgac gccgtagata ccagccgctt ccatttccac gccgaggatg ccgtattttt
    18241 ccatcacgtc gaacatttcg ccgtccggag agtagaacag gtcagcggag aacaggttac
    18301 caacgcgagc atcaatgccc agtgctttag ccgcgtctac cgcgttacgc accatgtcaa
    18361 agtcagcgat agcggcaaag tcatgatctt taaaacggat gcggttaact ttggaatcgg
    18421 tgcaggcacc cataccgata acgacgtcgc gcagttttac gtgcggcaga actgcgccac
    18481 aggaacccac gcggataatt ttcttcacgc cgaaatcggt gatcagttct ttggtgtaga
    18541 tggagcagga cgggataccc ataccgtgac ccattacgga aattttgcgg cctttataag
    18601 taccggtgaa gcccagcata ccgcgaacgt tgttcacttc acgggcatct tcaaggaaag
    18661 tttcagcaat atactttgca cgcagcgggt cgcctggcat caaaactacg tcagcgaaat
    18721 cgcccatttc tgcattaatg tgtggggtag ccattgtttt atccttatct acatgttttc
    18781 accctctccc tgagggagag ggccggggtg agggcatcaa gccgcaccca ttatcagaac
    18841 atggctttgc catattccat atcagaagta ccaaaatatt ttgccagagt ctggccgata
    18901 tccgcgaagg tttcacggtg acccagtgag cccggtttta ctttcgggcc gtaaaccagt
    18961 accggaatgt gttcacgcgt gtgatcagta ccggtccagg tcggatcaca accgtggtca
    19021 gcagtgagga tcaggatgtc gtcatcgcgc agcagagaca tcagctccgg cagacggcgg
    19081 tcgaacagtt ccagacccgc cgcataacct gcgacgtcgc gacggtggcc ccaggaagag
    19141 tcgaagtcaa cgaagttggt gaagacgatg gtgttatcac ccgcttcttt catctctttg
    19201 atggtggcgt caaacagcgc gtccaggcca gttgctttca ctttcttggt gataccgcag
    19261 ttggcgtaga tgtctgcaat tttaccgaca gaaaccacct gaccgtgttt ttcatcaacc
    19321 agtttctgca gcacggtcgg tgctggcggc tcaacagcca ggtcgtgacg gttaccggta
    19381 cgctggaagt taccggcttt gtcgccaata aacggacgag cgataacacg accgatgttg
    19441 tagccgccgt tggtcagctc ttcacgggcg atttcgcaca gttcgtagag tttatccagg
    19501 ccaaaggttt cttcgtggca ggcaatctgg aacacggagt cagcggaggt atagaaaatc
    19561 ggcttgccgg ttttcatgtg ctcttcgccc agctgatcca gaatgaccgt accggaagag
    19621 tggcagttac cgagataacc aggcaggtta gcgcgttcaa ccagtttatc cagcagctct
    19681 tgcgggaagc tgttttcgtg atcggagaag tatccccact caaacagaac cgggacaccg
    19741 gcgatttccc agtgaccaga cggggtatct ttaccggatg acatttcgtg cgcccatgcg
    19801 tacgcgccga taacttcagc gttgccgtcc attcccgccg gaatgaaacc ggtagaacct
    19861 tcgtgtgctt tcgccagccc cagacgggtc agatttggca gattgagcgg gcctttacga
    19921 ccgttatcag cttcgccttt agcacaagct tctgcgatat gacccagggt gtcagccccg
    19981 acgtcaccaa agcgttctgc atcttctgta gcgccgatgc cgaatgagtc cagcaccata
    20041 ataaatgcac gtttcatatg ttctccgtac tcagtgcttc aaatttaaat gatcagatca
    20101 gtataccgtt attcgctgat acggcgatag acagttggtg tgctttccgg tgctttatcg
    20161 gcaagtttaa ttgccgcttt caccgctttc gccgcttcct gccagctgtt ttcgtctttc
    20221 gcgtggataa ccgccagcgg acgctgaccg tctacctggt cgcccagacg cgccatatca
    20281 gtaaagccga cgctgtaatc aatggtgtca gatgcctgac gacgaccgcc gcccattgca
    20341 accactgcca tccccagcgc gcgggtatcc atttcactga caaaaccttc ggtatcagca
    20401 tagactgctt tcgtcagcat cgctgtcggc agatacttcg cgtagttctc aacgaagtcg
    20461 gttgggcctt tttgtgccgc taccatacga ccaaagactt ctgccgcttt accgttgtcc
    20521 agcactgctt gcaatttcgc gcgcgcttcg gcgtcatctt tcgccagttt gccggagata
    20581 agcatctcca cgcacagcgc catcgtgaca tcaaacagac gcgggttacg atattcaccc
    20641 gtcaggaact gcaccgcttc acgaacttca accgcgttac ctgcactgga ggccagtacc
    20701 tgattcatat cggtgagcag cgcggtggtg cgcacgccag cgccgttagc cacgccaaca
    20761 atcgcttcgg caagggcttc agagagttcg taggtcggca taaacgcgcc gctacccact
    20821 ttcacgtcca tcaccagcgc atccagacct tccgccagtt tcttcgccag gatcgaggca
    20881 gtgatcagcg ggatggagtc cacggttgcg gtaatatcac gggtcgcgta gaaacgttta
    20941 tccgccggag ccagtgagct ggtctggccg ataatcgcta cgccgacgtc tttaataatt
    21001 tcgcggaaac ggttgtcatc cgggaaaatg tcgaagccag ggatggattc cagtttgtcg
    21061 agcgtaccgc cagtatgacc gaggccgcga ccggagatca tcggaatata gccgccgcag
    21121 gctgcgacca tcggccccaa catcagcgaa gtcacatcgc cgacgccgcc ggtggagtgt
    21181 ttatcaacaa tcgggccatt cagatgcagg cttttccagt cgagaacggt tcctgaatct
    21241 cgcatcgcca tggtcagcga gacacgctca ggcattgtca tatcgtggaa gaaaatggtc
    21301 atcgcgaggg cggcaatctg cccttcggag atagtgttgt cgcgaatacc gttgataaag
    21361 aaacgaattt cttcatcgct tagcgcatga ccatcacgtt ttttacgaat aatttcttgt
    21421 gcgagaaaca aggtaacctc ccgaggaaaa gagtatgagg cggcttttgc cggatgcgtc
    21481 tacgccttat ccggcctacg ggtagatacg tctcgtaggc ctgataagcg cagcgcatca
    21541 ggcgtaaagc atcttactta gtagctgctg gcgctcttac cgtcaccgtg gcccagcgct
    21601 ttcaacaggc ttgccagcag gctggaagca ccaaagcggt agtgacgcgc atctgcccag
    21661 tcagcaccga acagttcatc tgcgatggcg agatatttct gcgcatcttc cgcagtacgc
    21721 acgccgcccg ctggtttgaa accaacggtt ttttctacgc ccatatcacg gatcacttcc
    21781 atcatgatgc gcgcgctttc cggcgtcgcg ttcacagcca ctttaccggt agaggttttg
    21841 atgaagtccg cacccgcttt gatggagatt tcagacgctt tacggatcag cgcttcgtct
    21901 ttcagttcgc cggtttcgat gatcactttc agcagtacat tcgctgccgc gcaagcctct
    21961 ttacaggctt tcaccaggtc aaaaccaacc tgctcgttac ccgccatcag cgcgcggtac
    22021 gggaacacaa cgtcaacttc atcagcaccg taggcgattg ccgcacgggt ttctgccagc
    22081 gcgatttcga tgtcatcgtt accgtgtggg aagttggtta ccgtagcgat tcggatttcc
    22141 ggggtgccct gctctttcag cgttttgcga gcaatcggga taaagcgagg atagatacag
    22201 atagcggcgg tattgccgac cggagttttg gcctgatgac acagagcaat tactttctcg
    22261 tcggtgtcgt cgtcattcag ggtggtcagg tccatcaatt tcagtgcacg caggctgctt
    22321 gctttcagat cagtcatttc attctcctgg cttgtcgcca ataaaattca ccttgcgagt
    22381 ttgttagtat tctaacatct actccgcaac acacttcgat atacatcaca attaaggaaa
    22441 tctacttaca agtttgcatc actgtaatgc gatctggttc aaataattca ctttcaaatg
    22501 aatgcgtcag tggtggcaaa cgcatcagga tcaaagtgaa catcacgaaa cttcttacaa
    22561 tggcgcatca ccttcggcat aaaaggaaaa gaagatgccc acttctcatg aaaatgcact
    22621 gcaacaacgt tgccagcaaa ttgtcaccag cccagtgctt agcccggagc agaagcgcca
    22681 ttttctggct ctggaagcag aaaacaatct gccttatccg cagcttcctg ccgaagcccg
    22741 ccgcgcgctg gatgagggtg taatctgcga tatgtttgaa ggtcatgcgc cgtacaaacc
    22801 gcgctatgtc ttacccgatt acgcccgttt tctggcgaac ggttccgaat ggctggagct
    22861 ggaaggcgcg aaagatcttg atgacgcact ctctctgctg accattcttt accaccacgt
    22921 accgtcggtc acatcgatgc cggtctacct ggggcaactg gatgcgttgt tgcaaccgta
    22981 tgttagaatt ctaacacaag acgagatcga tgttcgaata aaacgtttct ggcgttacct
    23041 cgacagaacc ctgccagacg cctttatgca cgccaatatc ggcccgtctg attcgcccat
    23101 tacccgcgcg atcttacgtg cagacgcaga gttgaagcag gtttcaccga acctgacctt
    23161 tatctacgat cctgaaatca cccctgatga cctgctgctg gaagtggcga agaacatctg
    23221 tgaatgtagc aaaccgcaca tcgccaacgg tccggtgcat gataaaattt tcacaaaagg
    23281 gggctacggg attgtgagct gttacaactc actgccgctg gcgggtggtg gcagcacgct
    23341 ggtacgcctt aacctgaaag ccattgccga gcgcagcgaa tcgctggatg acttctttac
    23401 gcgcactcta ccgcactact gccagcagca gatcgccatc atcgatgcgc ggtgtgaatt
    23461 cctctatcaa caatcacact tctttgagaa tagcttcctg gtgaaagaag ggctgattaa
    23521 ccctgaacgt tttgtgccaa tgtttggcat gtatgggctg gcggaagcgg ttaacttgct
    23581 gtgtaagaaa gaagggattg ccgcgcgcta cggtaaagaa gccgccgcaa atgaagtggg
    23641 ttatcgcatc agcgcgcaac tggcggagtt tgtcgccaat acccccgtga aatatggctg
    23701 gcaaaaacgc gccatgttac acgcacagtc ggggatcagt tccgatatcg gcaccacgcc
    23761 gggcgcgcgt ttgccgtatg gcgatgagcc agatccgatc acccatctgc aaactgtcgc
    23821 cccgcatcat gcttattatt attccggcat cagcgacatt ctgacgctcg acgaaaccat
    23881 caaacgtaac ccgcaggcac tggtacagct ttgcctcggt gcctttaaag ccggaatgcg
    23941 tgaatttacc gccaatgtca gcggtaacga tctggttcgc gttaccggtt atatggtgcg
    24001 tttgtcggac ttagaaaaat atcgcgccga aggttcacgc accaacacca cctggctggg
    24061 cgaagaagcc gcacgcaaca ctcgtattct ggaacgccag ccgcgcgtga taagccatga
    24121 acagcagatg cgctttagtc agtaagatta tccccttctc ctgcgttgac gggccaggca
    24181 gtcgtctggc tctgtttttg cagggctgca atctgcgctg caaaaactgt cacaatccgt
    24241 ggacgatggg acgttgcaat gactgtgggg agtgcgtgcc acagtgtccg catcaggcgt
    24301 tgcagattgt tgacggcaaa gtggtgtgga acgctgtggt ttgcgagcag tgtgatacct
    24361 gcctgaagag gtgtccgcaa catgccacgc caatggcgca atccatgagc gtggacgaag
    24421 tgcttagcca tgtccgcaaa gcggtgctgt ttattgaagg gataacggta agcggcggcg
    24481 aagccacgac ccaactgccg tttgtggtgg cgctgtttac agccatcaaa aacgatccgc
    24541 aactgcgcca cctcacctgc ctcgtggaca gtaacggtat gttgagcgaa accggctggg
    24601 aaaaattact cccggtgtgc gacggcgcga tgctcgatct caaagcgtgg gggagcgaat
    24661 gtcatcagca attaaccgga cgcgataatc agcagattaa gcgcagcatc tgtttgctgg
    24721 cagagcgcgg taagctggcg gaactgcgtt tgctggtaat tccagaccag gtggattatt
    24781 tgcatcacat cgatgagctg gcgacgttta tcaagagact tggcgatgtt ccggttcgcc
    24841 tgaatgcgtt tcatgcccac ggcgtgtatg gcgaggcgca aagctgggcg agcgccacgc
    24901 cggaagacgt tgagccgttg gctgatgcgt taaaggtgcg cggggtgagc cggttgatat
    24961 ttccggcgct ctatttgtga catgccggat acggcctacg gttcggcaca aacctgtagg
    25021 catgataaga cgcgacaagc gtcgcatcag gcattatgcg ccgactgccg gatgctgcct
    25081 acgaagggca aactacccac taacgctaaa caccgcatac gtattattaa gcaacacttc
    25141 ggcaatctca tccgccggtt ccgagcgcaa ctcgcacagc acggcaaata cccgggcggc
    25201 ctgttccggg cggttagcct gcccctgaaa accattgagc ggcatatccg gcgcgtcggt
    25261 ttccagcaac aacgatgcca gcggtaattt tgcgatgaca tcgcgggttt tactggcgcg
    25321 tggataggtg atagtaccgc ctacgccgat tttgtagccc agctgtacaa accgttcggc
    25381 ctgttgcagg ctgccggaaa aaccgtgaac cacgccagtg cgcgataaat cgtggcgttt
    25441 aagatgcatc gccagtttgt cgtgtgtgcg ccgggaatga aggatcaccg gcagatcgta
    25501 gcgtttcgcc agtttcagct gttcgtcgag taaccactgc tgcctctcaa attgcggatc
    25561 gtcgccgaag agatccagac cgatctcccc caccgccacc accttcgccg gacgcctttc
    25621 cagcgcctgc tgtagctgat caagagacac atcgctatgt ttttccaaca tccccggatg
    25681 caaccccaat gcggcataaa gcggttgata tttttccgct aacgcctgca cgcgggcaaa
    25741 attttccgcc tctgtcgccg gaacaatgat cttgcctacg cccgcttgtg ccgcgcgttg
    25801 caggctggcc tcttcatcgc cactaaacgg cgggaaatca aaatggcagt gggtatcgat
    25861 aaaacggcaa atcaagccag atcctcattg ttaaatgtcg ggtcgttcgc ctgcggagca
    25921 tcgctcactt cggcaaccag cgtatcgttg gcgactggcg caggcggaat aacgatcgat
    25981 tccggcgtca ccactggcac cagatggcgg gtaagcggcg ctttttcagt taatagcttg
    26041 ccaaccgtgg cgaggaaata acgaccgcat aaacgcccaa gtttatagtc ttcacgcagc
    26101 gccggaatcc gactgccaag cgcgatacta tgtaatggct tcggtggata aatttcgaat
    26161 atccgcagct tgcccggtgg tttctcaata aattgctgaa tgtcacgata gctggtttca
    26221 tgatgctgca ccagattgac cagcggctgc aggctactgt cacccagcca gcgttccatg
    26281 cgtttgaacc actgcggcgt gtagtacatt tgtgacggca cagtgcggat gacgaccaac
    26341 gttttagcgc cctgccttgc cgcctcttta accggaatcg catcactgat cccgccatcc
    26401 aggtagttaa tgccttccag cgacactccg ctacgataaa agccaggtat cgcactggag
    26461 gcgcgaatca catccagcca gttttgtttg gttggtaaaa agtaattcgg cgcgtagtca
    26521 tcctgacgac aggcgcacat ataaaacgat ttgccgctgt caaacaaccg cgcggcggta
    26581 tccatttgca acggcatctg acttgcggtg gcctccacca gccagtcgag atcgataaga
    26641 tttcctccac ggacaaagcg caatggatcg aaaaattcgc gttttgtggt atagcgcatg
    26701 atgactttgc gcgcgtaacc gggctgattg cagatatacg ccgagaggtt ctgcgccccg
    26761 gcagatgtgc cgagataaag atcgaaagga ttaaactgcg cgcgcataaa ctcatccagc
    26821 acgccagccg tgaaaattcc acgctgtccg ccgccttcgc acaccagagc tattcgtcca
    26881 ggctggaacg gccttagcga caacggcgca atattaccaa gcgttacagg tattcgctgc
    26941 cccacctctg ccttcctgtt tttgttgtga aagaatgaca aagtaacgca atttattatt
    27001 cgctaaaacc gcaaaagcca gtccgctgga ctggctttgg ataaaatttt cttaatggtc
    27061 tgaaatctag ggtcgttttc ggcccatgaa caaactcacc aggaacagaa taatcccgac
    27121 gacaaagaca attttagctg cgcctgcagc ggtaccggcc agaccaccaa acccaagtgc
    27181 ggcggcgatt aacgcgataa ccagaaatat gatgccccaa cgaaacataa gcgtctcctt
    27241 taccatagtt aatgtcaccg cttgatatga gcgtgaaaat cactcaatgc gatattttta
    27301 gtgtggtgca catcgcgcct cccgacaaag ttcgggagga cgaattacga cgaattactt
    27361 agttttcaga tcatttttaa cgcttttcac accatctacc gctttggcga tactttcagc
    27421 acggtcgctc tgtgcctggg aatcgacggt accggagagc tgaaccacgc cgtcggtggt
    27481 ttcaactttc acatgacggg aagggacgat atcgtccgcc agcagtttgg ctttgatttc
    27541 actggtggtg gcggtgtcac ccgcgtagcc cttcaccgag ccttctttag cgtcgcgaac
    27601 gtgcagtttg tcgctgacag aggtcacccc ttcaacgcct ttcgccactt tcactgcctc
    27661 ttcggcctga gcctggcttt caacgaaacc gctcagggtc acgacttttt gatcggtttt
    27721 gacagagata tcggtgctct tgatgttgtc atgatccacc agcgccgcct tcactttcgc
    27781 ggtgatggcg ctgtcatcca tgaaattacc gactttattc atagagctat cgactttttg
    27841 ccctgcgctt tcattggtag tctgtgcgtt gttttccgcg taggcagagc cggtcgcgac
    27901 ggcagaggtc aacattacag ccagcagagt tttcgaaatc ttcagtcttg tcatagtcat
    27961 cgatttattc ctgtatgttt gctcgtaatt tgagcctggc aacacgaggt tgcattgctg
    28021 aatagggaga gacttcaccc tctacagaag tcaatggtcg ccatcacaaa agcgatgggt
    28081 gatgaataac gaccattaca gcctctgaat cagttattaa tatcggtaga atgacaatcg
    28141 acggctttag atactgatat ctacgcattg aacggtattt aacgccgtca gaaatgtcat
    28201 tactttgtta aatatagttc acaattttga aaccgctcgg gatatcagcg aaaacataag
    28261 caaaagtgaa tgttttaaga acattccgta agcggctaat aaggaaggga aattgacagg
    28321 gcgcagcggc taccgcgccc tggaggcaag aattaatgct cgcgggtctg gtggaactga
    28381 acgtcgggat aacgttcctg cgccaggcgc aggttgacca tgctggtagc gatgtaagcg
    28441 aggttatcgc cgccatcaag cgccagttgg ctttcgttct tacgcttgaa ctcttcgaat
    28501 ttcttcgcgt ccgcacattc tacccagcgg gcagtggcaa cgttgactga ttcatatact
    28561 gcttcaacgt tgtattcgct cttcaggcgc gataccacca catcaaactg cagcacacca
    28621 acagcaccaa cgatcaaatc gttgttggag atcggacgga acacctgcac cgcgccctct
    28681 tcggaaagct gtaccagccc tttgagcagc tgtttttgct tcagcggatc tttcaggcgg
    28741 atacgacgga acagttctgg tgcgaagttc ggaataccgg tgaacttcat catctcaccc
    28801 tgggtaaagg tgtcgccgat ctgaatagtg ccgtggttgt gcaggccgag gatatcgccc
    28861 ggatacgctt cttcaacgtg cgaacggtcg cccgccataa aggtcagcgc gtcggagata
    28921 accacatctt tcgcagtacg cacctggcgc agcttcatgc ctttttcata tttaccggat
    28981 actacgcgca taaacgccac gcggtcgcgg tgtttcgggt ccatgttggc ctgaatttta
    29041 aatacgaagc cggtaaattt gtcttcgctc gcctctacgg tacgggtatc agtctgacgc
    29101 ggcatcggcg caggagccca ctccaccagg ccatccagca tatgatcaac accgaagtta
    29161 cccagcgcag taccgaagaa taccggagtg atttcgcccg caaggaacag ttctttgtcg
    29221 aactcgttag acgcgccttt caccagttcc agttcgtcac gtagctgctg tgccagatct
    29281 tcaccaaccg cagcgtcgag atccgggtta ttcagccctt taacaatacg gacttcctga
    29341 atcgtgtggc ctttaccgct ctgatagaga taggtttcgt ctttataaag gtggtaaacg
    29401 cctttaaaca gcttgccgca gccaatcggc caggtgatcg gtgcgcagcc gattttcagc
    29461 tcgttctcaa cttcatcgag cagttccatc gggtcgcgga tatcacggtc aagtttgttc
    29521 ataaaggtga ggatcggcgt gtcgcgcaga cgggtaactt ccatcagctt acgggtacga
    29581 tcttcaacac cttttgcagc gtcgataacc atcaggcagc agtccaccgc cgtcagggta
    29641 cgataggtat cttccgagaa gtcttcgtgc cccggggtgt cgagcaggtt aaccaggcaa
    29701 tcgtgatacg gaaactgcat cacagacgta gtaatggaga tcccacgctg cttttccatc
    29761 tccatccagt ccgacttagc gtgctggttg gaaccacggc cttttactgt accggcggtc
    29821 tgaatggcct gtccgaacag cagcaccttc tcggtgatgg tagtcttacc ggcatccggg
    29881 tgagaaataa tggcaaaagt gcggcgcttc gccacctctt gcaaataagg agacaacgtc
    29941 ataattcttt cttcttgagt aagcggcatc gcgccgcgct tgttgaaaac gaaaaattgc
    30001 ggctatttta cccatcaacg ggggggaggc aatcagtgtt tacacaggag ctgctccagt
    30061 tcgtgcaacg aagaaacggt ccaggtgggc gcgatgcctt ctggttgctc gcgatggtgc
    30121 gcattcagcc agcaggtcgc aagcccggcg ttgatgccac cgagaatatc ggactcggca
    30181 gtgtcgccaa ccatcagcac gcgtgaacgg tcaggattgc ccgcctgttc cagcgcataa
    30241 tcgaaaattt tcttattcgg tttggcaacg ccaacttctt cggaaatcac cagcaaatcg
    30301 aagtaatcac gcaggcccgt gcgttccaga cgcacttgct gcaaggcgct aaagccgttg
    30361 gtgatgatgc cgattttggc gttgccacga atggcgttaa gcagagaaac cgcgcccggc
    30421 agcggcgtgc agatttccgc catcgcatta ataaaggcat cgttaagcaa gcctgcttca
    30481 actttcagcc gttcggccca gctctcaaaa cgcccgtgct gaagctgtaa tgaagtgatc
    30541 gcgccgtttt gataatccac ccacagtggc ttgttaacgg cctgatagtc ctgaaaatct
    30601 tcagcggtaa aggtgacgct gtaatcaaga aacatccgct gcaggccggt gaatgagtca
    30661 aaggtaaaca gcgtttcatc ggcatcaaag aaaatccagt cccacttcat tattccacct
    30721 tgtcttacat actgattggc aacgccatga tgatggcgtc ttcgcgaccg tccgtggtgg
    30781 ggtagtaatt gcggcgaatc gtcgcctcgt taaagcctaa actttcgtac agggcaatgg
    30841 cggcagcatt tgaggcacgg acttccagcc atagtgtcgc cacgccgcgt ttttccagtt
    30901 cgtcgatcag atgttccagc agcacccttc ccaatccctg acgctgatag tcaggatcga
    30961 ccgcaatatt gaacaatgta gcttcatcca gcaccacttg cgtaatcgca aacgccgcca
    31021 ttttgccgtt ttgcgttaac tgaaagttga gataacgctc gccctggttg ctggcaaagg
    31081 ttttttcact ccacggaaag gcgtgggcgc gttgttcaat gtggtaagcc gctggtaaat
    31141 cagtcgtttc gagggaagaa atcgtgttca tatgtgcaaa tttgttgcca taacgcggcg
    31201 cgtgccgttg ggtttgcccg taattcggtg agcgccggtg atgccacctg agcgccttcc
    31261 agtgatagcg gttcgtcagt acccaaccgc cagctgttgc agcgactgcc ttgcggcagc
    31321 atcgcgattt tttctggcgt cagttgcagc acttgatcgg ggctgacggt taatgcgcgc
    31381 agaacatcgc tcactaaagg atcggtcagg gcgggaagat cgtttgccac catcaccaga
    31441 cggacgtgtg ccgggatcgc aatggcaatc tccccctgca acgcgccagg gcgacgcagc
    31501 gaccactggg taatgcccag ttgctgtaac tgccagtctc gtcgggatgt catagcgaat
    31561 cgctcctgtt gtcaggggcg caaatatagc aaattcgtcg ataccgcgcc aacatatggc
    31621 tataatcgcc gccagtatca attgaggagc attccatgtc tgcatttacc ccggcaagtg
    31681 aagtcttgct gcgtcacagt gatgatttcg aacaaagccg tattctgttt gccggagact
    31741 tacaggatga cctgcccgcg cgtttagata ccgcggccag ccgtgctcat acccagcaat
    31801 tccaccactg gcaggtatta agccgccaga tgggggataa cgcccgtttc agtctggtcg
    31861 ccacggcgga tgacgtcgca gattgcgata cgctgattta ctactggccg aagaacaaac
    31921 cggaagccca gttccagttg atgaatttac tttctctgct gccggtgggg acggatattt
    31981 ttgtcgttgg cgagaaccgc agcggcgtgc gcagcgccga gcagatgctg gcagattatg
    32041 cgccgttgaa taaagtcgac agcgctcgtc gctgtggcct ctattttggt cgtctggaaa
    32101 aacagccggt atttgatgcc gataaattct ggggcgaata cagcgtcgat ggcctgacgg
    32161 tcaaaacgct gcctggcgtg tttagccgcg acggtctgga tgtcggtagc cagttgctgc
    32221 tctcgacgtt aactccgcac acgaaaggta aagtgctgga tgtcggctgt ggcgcggggg
    32281 tgctttcagt tgcctttgcg cgccattcgc cgaaaattcg tctcaccttg tgcgatgtct
    32341 ctgcgccagc ggtagaagcc agccgcgcaa cacttgcggc caactgtgtt gaaggtgaag
    32401 tctttgccag caacgtcttt tccgaggtga aaggtcgttt tgatatgatc atctccaacc
    32461 cgccgttcca cgatgggatg caaaccagcc tggatgcggc gcaaacgctg attcgcggcg
    32521 cggtgcgtca tcttaatagc ggcggcgagc tgcgaattgt agcgaacgcc ttcctgcctt
    32581 acccggacgt gctggatgag acatttggct tccatgaagt gattgcgcaa accgggcgtt
    32641 tcaaggtgta tcgcgccatt atgacccgcc aggcgaagaa aggttaatta tctcgccgaa
    32701 taccgtgtcg gatgcggcgt gaacgcctta tccgacctac aaaacctgca cgttagccct
    32761 tcgtaggcca gataagacgc gccagcgtcg catctggcat ctgcccccgc atgcctacca
    32821 aacctgcacg ctagtttcct gatggacatt tttccagcaa ttacacctcc ggcgataatt
    32881 aactattgac gaaaagctga aaaccactag aatgcgcctc cgtggtagca attcttttta
    32941 agaattgatg gtatgcgaag gtggcggaat tggtagacgc gctagcttca ggtgttagtg
    33001 tccttacgga cgtgggggtt caagtccccc ccctcgcacc aattatcttt acttcctttc
    33061 ttgtttcttc cttgattttt catccctgat tcaaattcat tatcatcagc atgatggcag
    33121 caataagcgc caggcacgat ggtagcaaaa cgtaatgccg taactgtttg tgatacagca
    33181 tcaccgacgt caatactaat cccatcagaa tgacaatccg ccactgatcc ggtgataaat
    33241 ccgcatacat cagccctgca atcagcaatc ctggcagcaa cactccccag ccactgccca
    33301 gcgtacgttg caacatggtt tcatctcttt cattgataat gataaccaat atcatatgat
    33361 aatttttatc atttgcaagc cagagaaatc ccttgctatc gggtaaacct atcgctagga
    33421 ttagcaatca ttatcattta gattgctatc ccgattatgg cctatcgttc cgcaccgctc
    33481 tatgaagatg ttatctggcg aacgcatctc cagccacagg atgctgggct tgcacaagcc
    33541 gtacgtgcga cgatcgccga acatcgtgaa catttgctgg agtttatccg cctggatgaa
    33601 cccgctccgc ttaacgccat gacgctggcg caatggtcat caccgaatgc gctaagttct
    33661 ctactcgcgg tctattccga tcatatctat cgcaaccaac caacgatgat ccgcgagaac
    33721 aaaccgctga tctcactatg ggcacaatgg tatatcggcc tgatggtgcc accattaatg
    33781 ctggcgctac tgacgcagga aaaggcatta gatgtgtcgc cggaacattt ccatgctgag
    33841 tttcacgaaa ccggccgtgc cgcctgtttc tgggtcgatg tgtgtgaaga taaaaacgca
    33901 acaccacatt cgccgcagca gcgaatggaa aggttaatca gccaggcgct ggttccggtt
    33961 gtgcaggcac tagaagcgac cggagagatc aacggcaaac ttatctggag taataccggt
    34021 tatttgatca actggtatct tactgagatg aagcaactgc tcggcgaggc gacggttgaa
    34081 tcgctgcgcc atgccctctt ttttgagaaa acgcttacta ccggtgaaga taatccactc
    34141 tggcgtaccg tggtgctacg cgacggcctg ctggtgcgcc ggacctgctg ccagcgttat
    34201 cgcttaccgg atgtgcagca atgtggcgat tgtacgctga aatgaaagca ctgccgggga
    34261 agtaaacccg gcatcatgca gattaatagg gatgcaacac attacttgct tcgcaatgcc
    34321 gcatacataa cagaatatct gcggcctcca acaaccctgc atcagaactg acgcccagtt
    34381 tcgacatcac attaaattta tgtgcacgga tcgttttgat attgcgttta agctgctcgg
    34441 caatttgtgt cattgagtag ccacgcgaca taaagcgcaa tatttctctc tccgtcgggc
    34501 ttagcgtccg gctttggtta atgtaccact gattgttcag tcggtcggtc gcctgacgta
    34561 caccatttaa tgacaaaaag agttcctgat gaaaaatctc cagcgttgac gctttactta
    34621 atacaccgtc cagcggtgat ggcgacaatg aaccaatcag ccgagcttca atatcatcat
    34681 ccgcaataac taaacgccgg gtacgcggaa acttaatcgc cagttcagtc aggcaagata
    34741 atccctctct gcgctcactt ctcatggcag aaaaagaaaa aatgaccgcc gcaaatgaga
    34801 tccgcgacat ggcatcctga aatgactcct gactggtata ggtatggagc ttataagggt
    34861 tacccgcaaa ctttctgaat aaactctcaa taccgatact gctcatgacg catttttcta
    34921 tgagtgcgac atttctcttc ttaattcggc tgtgttccat tctgcggcat ctccataaac
    34981 agaaggcaag ttaagttctc ttgcaccctg cgcgctattt atccatgcat acatttcagc
    35041 gttgctgtga attgccagtc gacgcatcgc actgtttttt tgggcgctga tagttttatt
    35101 acttttctta agcaacgaag ctatctggtt gatgccccat cctttaccta atagccgcag
    35161 taccttgcgc tctgaaagag tgagaatgac ggagcgatag ctaaaatcat gagtttcatt
    35221 cattgccgga gaggtcagca tggcgctgat acgctctgcg tgcgtattgc cggaacgtac
    35281 ggtcttgacc agattctcaa tgggttcaac atcagacaat aacgtcgccg tagggcacat
    35341 gagcaattct actgcctggg aataccagga gcgtgacacc atgaaaaccc agtgaatttc
    35401 ccgatattgt gagattaaag aataataatg ttcacaaaca gaacgaggat cttcactttc
    35461 accggctaaa tcagcaatga ctaatccgga acgacgtaat tgtaataagg taaggtcctc
    35521 cgcagaggcg ctggatatta tttcatattc agggaagtga gtcctcatga cctcttttaa
    35581 ccctgcttgc ataacaggta ttttactgat aacaattcca tttttgcagc atcctggcaa
    35641 catatttcct ccacatccac tgtctaaaaa tcatgatggt ttattcagga tattcattcc
    35701 ttaaatgttt ccagcaaatt aatagattaa gcgaataaat tatccttagc attcactttc
    35761 atatcagcat actcctgctg cacaacaact ccactgcatg tgtcagaagg ttagcatcaa
    35821 atgataacct ggtgaaatag cctggagaca tttaaaatat ctacattaag ttgttaggaa
    35881 tacttaatga a
//
LOCUS       DAPHDW010000034        35656 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.034, whole genome shotgun sequence.
ACCESSION   DAPHDW010000034 DAPHDW010000000
VERSION     DAPHDW010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 35656)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35656)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35656
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.034"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(47..778)
                     /gene="rlmB"
                     /locus_tag="RH057_001660"
     CDS             complement(47..778)
                     /gene="rlmB"
                     /locus_tag="RH057_001660"
                     /EC_number="2.1.1.185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HDV3128046.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACLRSADAAGVHAVIVPKDRSAQLNATAKKVACGAAESVPLIRVTNLART
                     MRMLQEENIWIVGTAGEADHTLYQSKMTGRLALVMGAEGEGMRRLTREHCDELISIPM
                     AGSVSSLNVSVATGICLFEAVRQRS"
     gene            complement(958..3399)
                     /gene="rnr"
                     /locus_tag="RH057_001661"
     CDS             complement(958..3399)
                     /gene="rnr"
                     /locus_tag="RH057_001661"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HDV3128047.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRRQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(3438..3863)
                     /gene="nsrR"
                     /locus_tag="RH057_001662"
     CDS             complement(3438..3863)
                     /gene="nsrR"
                     /locus_tag="RH057_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HDV3128048.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKSASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(4068..5366)
                     /gene="purA"
                     /locus_tag="RH057_001663"
     CDS             complement(4068..5366)
                     /gene="purA"
                     /locus_tag="RH057_001663"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HDV3128049.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPTELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(5470..5667)
                     /locus_tag="RH057_001664"
     CDS             complement(5470..5667)
                     /locus_tag="RH057_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HDV3128050.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(5749..6753)
                     /gene="hflC"
                     /locus_tag="RH057_001665"
     CDS             complement(5749..6753)
                     /gene="hflC"
                     /locus_tag="RH057_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HDV3128051.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(6756..8015)
                     /gene="hflK"
                     /locus_tag="RH057_001666"
     CDS             complement(6756..8015)
                     /gene="hflK"
                     /locus_tag="RH057_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HDV3128052.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGTSNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(8101..9381)
                     /gene="hflX"
                     /locus_tag="RH057_001667"
     CDS             complement(8101..9381)
                     /gene="hflX"
                     /locus_tag="RH057_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HDV3128053.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKATGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(9458..9766)
                     /gene="hfq"
                     /locus_tag="RH057_001668"
     CDS             complement(9458..9766)
                     /gene="hfq"
                     /locus_tag="RH057_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HDV3128054.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(9852..10802)
                     /gene="miaA"
                     /locus_tag="RH057_001669"
     CDS             complement(9852..10802)
                     /gene="miaA"
                     /locus_tag="RH057_001669"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HDV3128055.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSA
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(10795..12642)
                     /gene="mutL"
                     /locus_tag="RH057_001670"
     CDS             complement(10795..12642)
                     /gene="mutL"
                     /locus_tag="RH057_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HDV3128056.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMNVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFNHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRSIPENRVAAGRNHFAEPAAREPVAPRYTPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSAEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(12652..13989)
                     /gene="amiB"
                     /locus_tag="RH057_001671"
     CDS             complement(12652..13989)
                     /gene="amiB"
                     /locus_tag="RH057_001671"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HDV3128057.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGVIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADAPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVARKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKQSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(14008..14469)
                     /gene="tsaE"
                     /locus_tag="RH057_001672"
     CDS             complement(14008..14469)
                     /gene="tsaE"
                     /locus_tag="RH057_001672"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HDV3128058.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(14441..15988)
                     /gene="nnr"
                     /locus_tag="RH057_001673"
     CDS             complement(14441..15988)
                     /gene="nnr"
                     /locus_tag="RH057_001673"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HDV3128059.1"
                     /translation="MTDHTMKKNPVSIPHTVWHADDIRRGEREAADALGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLATAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIAAVDIPSGLLAETGATPGAVINADHTITFIALKPGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPGEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15987..17126
                     /gene="queG"
                     /locus_tag="RH057_001674"
     CDS             15987..17126
                     /gene="queG"
                     /locus_tag="RH057_001674"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HDV3128060.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGAIPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEEDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(17109..17162)
                     /locus_tag="RH057_001675"
     CDS             complement(17109..17162)
                     /locus_tag="RH057_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128061.1"
                     /translation="MRFYFYSQAVDESGVTR"
     gene            complement(17396..17471)
                     /locus_tag="RH057_001676"
     tRNA            complement(17396..17471)
                     /locus_tag="RH057_001676"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17436..17438),aa:Gly,seq:gcc)
     gene            complement(17508..17583)
                     /locus_tag="RH057_001677"
     tRNA            complement(17508..17583)
                     /locus_tag="RH057_001677"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17548..17550),aa:Gly,seq:gcc)
     gene            complement(17794..18339)
                     /gene="orn"
                     /locus_tag="RH057_001678"
     CDS             complement(17794..18339)
                     /gene="orn"
                     /locus_tag="RH057_001678"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV3128062.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            18434..19486
                     /gene="rsgA"
                     /locus_tag="RH057_001679"
     CDS             18434..19486
                     /gene="rsgA"
                     /locus_tag="RH057_001679"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV3128063.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            19583..20551
                     /gene="psd"
                     /locus_tag="RH057_001680"
     CDS             19583..20551
                     /gene="psd"
                     /locus_tag="RH057_001680"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV3128064.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            20573..23896
                     /gene="mscM"
                     /locus_tag="RH057_001681"
     CDS             20573..23896
                     /gene="mscM"
                     /locus_tag="RH057_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV3128065.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(23925..24239)
                     /locus_tag="RH057_001682"
     CDS             complement(23925..24239)
                     /locus_tag="RH057_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDV3128066.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(24236..24550)
                     /locus_tag="RH057_001683"
     CDS             complement(24236..24550)
                     /locus_tag="RH057_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128067.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(24602..26104)
                     /gene="yjeM"
                     /locus_tag="RH057_001684"
     CDS             complement(24602..26104)
                     /gene="yjeM"
                     /locus_tag="RH057_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV3128068.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(26323..27300)
                     /gene="epmA"
                     /locus_tag="RH057_001685"
     CDS             complement(26323..27300)
                     /gene="epmA"
                     /locus_tag="RH057_001685"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV3128069.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            27625..29433
                     /gene="frdA"
                     /locus_tag="RH057_001686"
     CDS             27625..29433
                     /gene="frdA"
                     /locus_tag="RH057_001686"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV3128070.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            29426..30160
                     /gene="frdB"
                     /locus_tag="RH057_001687"
     CDS             29426..30160
                     /gene="frdB"
                     /locus_tag="RH057_001687"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV3128071.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            30171..30566
                     /gene="frdC"
                     /locus_tag="RH057_001688"
     CDS             30171..30566
                     /gene="frdC"
                     /locus_tag="RH057_001688"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV3128072.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            30577..30936
                     /gene="frdD"
                     /locus_tag="RH057_001689"
     CDS             30577..30936
                     /gene="frdD"
                     /locus_tag="RH057_001689"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV3128073.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            30999..32132
                     /locus_tag="RH057_001690"
     CDS             30999..32132
                     /locus_tag="RH057_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV3128074.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGEAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            32221..32754
                     /gene="blc"
                     /locus_tag="RH057_001691"
     CDS             32221..32754
                     /gene="blc"
                     /locus_tag="RH057_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV3128075.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(32751..33068)
                     /gene="sugE"
                     /locus_tag="RH057_001692"
     CDS             complement(32751..33068)
                     /gene="sugE"
                     /locus_tag="RH057_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV3128076.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(33250..33396)
                     /gene="ecnB"
                     /locus_tag="RH057_001693"
     CDS             complement(33250..33396)
                     /gene="ecnB"
                     /locus_tag="RH057_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV3128077.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(33507..33632)
                     /gene="ecnA"
                     /locus_tag="RH057_001694"
     CDS             complement(33507..33632)
                     /gene="ecnA"
                     /locus_tag="RH057_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV3128078.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(33684..34250)
                     /gene="efp"
                     /locus_tag="RH057_001695"
     CDS             complement(33684..34250)
                     /gene="efp"
                     /locus_tag="RH057_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV3128079.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            34292..35320
                     /gene="epmB"
                     /locus_tag="RH057_001696"
     CDS             34292..35320
                     /gene="epmB"
                     /locus_tag="RH057_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV3128080.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLHQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         8494 a         9782 c         9054 g         8326 t
ORIGIN      
        1 aaaaaaaacc atccaaatct ggatggcttt tcataattca gacgaactag ctgcgctgac
       61 gaaccgcttc aaataagcaa attccggttg caaccgaaac gttcagggaa gaaacacttc
      121 ctgccattgg gatgctgatc aactcatcgc aatgttcacg ggtcaggcga cgcatacctt
      181 caccttccgc gcccatcacc agcgccaggc gtccggtcat tttgctctga tagagtgtat
      241 gatccgcctc gcctgccgta ccgacgatcc agatattctc ttcctgcaac atacgcatgg
      301 tgcgcgcaag gttagtcacc cgaatcagtg gaacgctttc tgccgcgccg caggctactt
      361 ttttcgccgt agcgttgagc tgtgcggagc gatctttcgg cacaatcacc gcgtgaacgc
      421 cagcagcgtc cgcgctacgc aggcacgcgc cgaggttgtg cggatcggtt acaccgtcga
      481 ggatcagcag gaacggttga tcgagcgaag cgatcagatc cggcagatcg ttttcctgat
      541 actgacgtcc tggcttcacg cgggcgataa tgccctgatg cacggcaccg tcgcttttct
      601 cgtcgagata ttggcggttt gccaactgga taaccacgcc ctgagactca agggcgtgaa
      661 tcagcggtaa cagacgttta tcttcacggc cttttaaaat aaagacttcc tgaaaacgtt
      721 caggggcgcg ctccagcagg gcctgcactg cgtggatgcc gtaaatcatt tcgctcatta
      781 atgtactcgt tgtttacgtt ttgcctgatg cactacgttt atccggccta catgatctct
      841 gcaatatatt gaatttgcgt gattatgtag gccggataag gcgttcacgc cgcatccggc
      901 atgaacaaag cgcattttgt cagcaatcta accctcttct tttaaagagg gtattgatca
      961 ctctgccact tttttcttcg ccgcacgctt cgctttggtc gctgcggcta ttttctgcgt
     1021 tttcgccgat ggctttttcg cttttctcgc gtctttcttc gccgctttcg gcttcgtttt
     1081 tttctcaccg cggaaggcgc tgtctggctc aaagtttacc tttttaccga cctgacgacg
     1141 cttgccgcct tttttgcctg catcgccttt tttcgctttc tcgcgcgccg ttttaccgac
     1201 gttgcgcggt gcgcgttcac tggagatcag actaaagtcg attttgcgct cgtccatatt
     1261 aaccgcttcg actcgaactt ccacgcgatc gcccaggcga taagtctggc cgctggattc
     1321 ccccatcagg cgttgcccta cctggtcaaa gcgatagtag tcattgtcca gcgaagagac
     1381 atggaccaga ccatcaatga acaagtcgtc cagacggacg aagaagccaa agccagtgac
     1441 gctggaaatt acgcctttaa agacgttacc tacctggtcg agcatgaagt cacacttcag
     1501 ccagtcagcg acatcgcgcg ttgcttcgtc ggcacgacgt tccgccatcg aacagtgctg
     1561 acccagttgc aacatctctt ccatcgaata atggtagccg cctgtttcag tggtgttgcc
     1621 ctgatgcccc tgctctttcg ccagcagata tttaatggcg cggtgcagcg tcaggtcagg
     1681 ataacgacga atcggcgaag taaagtgcgc ataggactgc aatgccagac cgaagtgacc
     1741 acggttttct ggatcgtaaa tcgcctgttt catcgagcgt agcagcatgg tttgcagcat
     1801 ttctgcatca ggacggtcgg caaccgactc cagcagttcc gcgtagtcac gcggttccgg
     1861 cttgttacca cccggcagct ccagccccag ctccgccagc actgaacgga aagaggtaat
     1921 cgcttcggtg ctcggcttgt cgtgaatacg gaacagtgcc ggttctttcg ctttctcaac
     1981 gaaacgcgcc gccgagatat tcgccagaat catgcactct tcaattaatt tgtgcgcgtc
     2041 gttacgctgg gtctgttcga tacgttcaat acggcgttca gcgttgaaaa tgaacttcgc
     2101 ttcttcgctc tcaaatgaga tcccaccgcg ttcttcacgg gctttatcca gcactttata
     2161 gaggttatgc aactcttcga gatgcttaac cagcggggcg tactgctcgc gcagatcctg
     2221 atcgccctgc agaatatgcc agactttggt gtaggtcaga cgcgcgtgag agctcatcac
     2281 cgcttcgtag aatttgtagc ccgtcaggcg gcctttcgac gaaaccgtca tctcgcacac
     2341 catacacagg cggtctacct gcgggttgag cgagcacagg ccgttagaga gcacttccgg
     2401 cagcatcggg ataacctgcg aagggaagta caccgacgta ccacgattac gcgcttctct
     2461 gtccagcggc gttggcggac gcacatagta gctgacgtcg gcaatcgcga cccataaacg
     2521 ccagccgccg ccgcgttttt tctcgcagta aactgcatcg tcaaagtcac gggcgtcttc
     2581 gccatcaatg gtgaccagcg gtaaatcgcg caaatcgaca cggcccgctt ttgcttcttc
     2641 cggcacttct tctttcagcc cggcaacctg ttgctcaaca gcctgcggcc agatgtacgg
     2701 aatttcatgg gtacgcagag cgatatcaac cgccatgccg gtgcccatat tatcgccaag
     2761 gacttcgacg attttaccca ccgctttggt acggcgagtc gggcgttggg tcagttcaac
     2821 aaccaccaca aagcccatcc gcgcgcccat gatctgatcg ggcgggatta agatatcgaa
     2881 gctcagacgg ctgtcgtcag gaaccacaaa gccgacgccc gcttcggtaa agtagcgacc
     2941 aacaatctgg ctggtttttg gcaccagtac gcggacaata cgcgcttcac gacgaccttt
     3001 acggtcagcg cccagcggct gagccagcac ctgatcgcca tgaatgcagg ttttcatctg
     3061 ctcgctggag agatacaaat catctttacg cccttcaacc cgcagaaagc cgtagccatc
     3121 acggtggcca ataacggtac ctttcaccag gtcgaggcgt tccggcagcg catagcactg
     3181 acgacgagtg aagaccagtt gaccatcgcg ctccatcgcg cgcaggcggc gacgcaggcc
     3241 ttcaagctgc tcttcgcctt caatgtgcag ttctaccgcc agctcatcac ggctggccgg
     3301 tttttcacgt ttagttaaat gttcgaggat aaattcccga ctagggatgg gattcgcgta
     3361 tttttcagct tcgcgttcct ggaaaggatc ttgtgacatc tcggttcctc cgttgtcatc
     3421 tctgatgaag attttcgtca ctccaccagc aataatttat aaagcggttg attctcttca
     3481 accaaatcgg caagcgtgta gttatccagt tccgtaagaa aactttgcac ggccttagaa
     3541 agcgcctgtt tcaaccgaca ggcaggtgta atgtggcaaa actcactgct gcaattcacc
     3601 agcgataagg gctccagctc gcgcaccaca tcaccaatac gtatcgcact cgccgattta
     3661 cccaggcgaa tgccgccatt ttttccacga acagcagtca cgtagccggc acgactaagt
     3721 tgattgatta ttttgaccat atgattacgg gagacgccgt agacgtcagt cacttcagaa
     3781 atacttgtca tccgcccttc tggcaatgac gccatgtaga tcagcgcacg taatccgtaa
     3841 tcagtgaaac tcgttaactg cacatcaacc tcaaaaggga aatcgggaaa aaatacattt
     3901 atattgatga taaaccagcc acaagctgtg tcgctaattt atttcagttc ggggaaggaa
     3961 aaaagcgagg atttaacact gtgtcggata gcgtaatagc ttaaccgaca cagcaatgta
     4021 tcacactgtt agcccggcag gcaaaatatc tgccaggcgt accagaatta cgcgtcgaac
     4081 ggatcgcgca gaatcatggt ttcagtacga tccggaccgg tagagatgat atcgatcggc
     4141 acaccagtca gctcttcaat acgcttgatg tagttcagcg ccgcctgcgg cagaccgcta
     4201 cgatctttca cgccgaaggt ggattcagac cagcccggca tggtttcgta aatcggctct
     4261 acacctttcc agtcgtcagc tgccagcgga gtggtcgtca cttcacgacc atccggcata
     4321 cggtaagcca cacagagttt cacctctttc aggccatcca gaacgtccag tttagtcagg
     4381 cagaagccag acagggagtt cagctgtacc gcacgacgaa cggcaacggt gtccagccag
     4441 ccggtacgac gacgacgccc cgtagttgcg ccgaattcgt taccctgctt gcagaggaac
     4501 tcgccagttt catcaaacag ttcggtcggg aacggacctg cacctacacg agttgagtaa
     4561 gctttgagga tacccagaac ataatcaaca taacgcgggc ccaggccgga accggtcgcc
     4621 acgccaccag cagtggtgtt ggaagaagtt acgtacggat aagtaccgtg gtcgatatcc
     4681 agcagcgtac cctgcgcacc ttcaaacatg acgaaatcgc cacgctgacg cgcctggtcg
     4741 agcaggtcag aaacgtcaac caccatagaa gtcaggatgt cggcaacagc catcgtatca
     4801 tccagaactt tctggtaatc aaccgcttca gctttgtagt agttaaccaa ctggaagttg
     4861 tgatattcca tcacttcttt cagtttttca gcgaaggttt ctttgtcgaa aaggtcgcca
     4921 acacgcagac cgcgacgtgc tactttatct tcataagcag gcccgatacc acgaccggtg
     4981 gtgccgatcg ctttcgcgcc acgcgctttc tcacgcgcgt tatccagcgc aacgtgataa
     5041 tcaaggatca gcggacatgc ttcagacagc agcagacgct cacgaacggg gatgccacgg
     5101 tcttccagtt ctttcatctc tttcatcagc gcggccggag acagcacaac accgttaccg
     5161 atgatgctgg ttacattctc gcggagaata cctgatggaa taagatggag aacggttttt
     5221 tcaccgttga ttacgagagt atggcctgcg ttgtgaccgc cctggtagcg tacaacatat
     5281 ttagcccgtt cagtcagaag atcgacgatc ttacctttac cttcgtcacc ccattgggtg
     5341 cccagtacga cgacgttgtt acccattttt caaaatcacc gtttgcttaa aaatggattc
     5401 taccatcgct ttttcagagt tacagcactt tttgcactca aaaatgacct cgatcagtct
     5461 gttttttgtt cagccaatcg ttttcctcaa catgtagtag accacaacgc ccgcaaccac
     5521 aagtccaccg ccaaaacgac gtaaaatatt atcgggcaaa ttggtcatcg cagagatcat
     5581 cttcttccat gccttcgggt aaagcatcgg ccctaaacct tccagtacca aaaccagggc
     5641 aagcgccagc cagattgtcg aattcattca ttatccttat agaaaaagaa aaccaccgac
     5701 atccctgagg atgcggtggc tttattgacc tgtaccgcag tcgttatatt aacgcgttgc
     5761 ggaagtcggc gtcttcatgt agcggaagaa atcgctatcc gggctcatga ccatcacgtc
     5821 ctgattgcca gagaagctgt tctcataagc acgcaggcta cggatgaatg cgtagaagtc
     5881 cggatcttta ctgaatgcat cagcaaacag tttggctgct tcggcatcgc cttcaccacg
     5941 catgatgcgg ccctgacgct cagcttctgc cagcgttctg gtcacttcat agtcggcagt
     6001 cgcgcgcagt ttttccgctt cttcctgacc ttgtgaacgg tgacgacgcg ctaccgcttc
     6061 acgctcggcg cgcatacggt tgtagatcgc ttcagacact tcggtcggca ggttgatctg
     6121 cttaattcgc acatcgacaa cctcaatacc cagcgccgcc atactgttcg ggttgatgac
     6181 cggaactttg cccttcgtct cagccgttac gcgctctgcc gcttcggcaa tggcgttatc
     6241 tgccgccggg gtagtaactt catcttctgt acccgcagaa ccggagttca gcgcgtcacg
     6301 tacttcgagg gtcagacgac cacgggaatc ggtgacgata tctttcacgt ccaggcgacc
     6361 aatttcagaa cgcagacggt cagagaactt acgtttcaac agcacttccg cttgcgaaat
     6421 gtcgccacca cccgttgcca ggtagtaacg gctgaaatcg ctgatgcgcc atttgatgta
     6481 agagtcgacg atcaggtctt tcttctcttt ggtcacaaag cggtcggcct ggttgtccat
     6541 ggtctgaata cgtgcgtcga gcattttcac cgtttcaatg aacggtatct tgaaatgcag
     6601 acccggctca taaaccagag gtttgttgtc atcgtcacgc agtaccttac caaaacgcag
     6661 cgtaataccg cgctcacctt ctttgacgac aaagacagac atgtaaagca ctaccagcac
     6721 gatgatgata atcgcgataa ctgacttacg catcgttatt ccccctgacg ctggtagtcg
     6781 ttacgctgcg cgttggcgcg gcgttggtcc ataatatcgc cctgactggt ggacgacgtg
     6841 ttgcttgctc cactggttgt ggaggaagag gctggcggca gacgcagcag attgctggta
     6901 ccgttatcgc tcttcgccgc aggggcgtta ccgcctttca gcatctggtc taacggcaga
     6961 accatcaggt tgccaccttt atcgttaacc agcactttgc gggtgttacc caacactttt
     7021 tccatcgtct cgatatacag acgctcgcga gtaatttccg gcgcggcttt atattccggc
     7081 agaagtttag caaagcgcgc cacttcaccc tgagcttcca ggatggtctg ggccttgtac
     7141 gcacgcgcct cttcgaggat acgttgcgcc tgaccgttcg cacgcggctg aacttcgttg
     7201 gtatacgctt ctgcttcacg aatgtattgc tgttcgtttt cacgcgcggc aatcgcatcg
     7261 tcaaacgccg cttttacttc ttccggcgga cgagcagcct ggaagttgac gtccagcagc
     7321 gtgataccca tgtcatacgg acggatagtc tcttccagtt cgcgctgagt atcgctacga
     7381 atcacggtac gaccttccgt cagaatgcgg tccatggtgt atttaccgat aaccccgcgc
     7441 agggcgctgt cggtagcctg acgcaggctg tcatccgggc tggtcacgct atacagatat
     7501 ttttccggat tggtgacgcg gtactgcacg ttcatctcaa cgcgcactac gttctcgtcc
     7561 gacgtcagca tcacaccaga agcggccagt tcacgcacgg cttccacgtt caccggtttg
     7621 acttcgtcga taaacgtcgg tttccagttc agacccggct caaccagatg gctgaattta
     7681 ccaaagcgtg ttaccacgcc gcgttcggct tctttaatgg tatagaaacc actggccgcc
     7741 cagataatga caatcgctgc cgctgcgatg gtaacgacac gaccgccaag ctgcgggcgc
     7801 gggccttgcg atgaactgcc accgccagat ccggtgcctt taccgccgcc cagaccaccg
     7861 agctttttgc tcagtttgcg gaagatatca tctaaatcag gtggcccttg atcgcgaccg
     7921 cctttgtttc catttccctc agagttgccg ccaggtttgc tgcttcccca cgggtcgcgg
     7981 tcttgtccgt tattaccggg ctgattccac gccatgtttg tgctccatat ttgttatgcg
     8041 gtgatccctg ttgtctttcg acgccagagg atatgactcc acgcttcaga cgctacgccg
     8101 ttagatcagg taatcgatca acgccggttc ttgtttacag aggcgacgcc agtcaacgat
     8161 cggcatacga acttgcagac ttacgctgcc gtcctcctcc atccactctt tttctattgc
     8221 ctgaagctga taaaaacgac ttctcagacg cccttcctgc ggtggcagac gcaatgtatg
     8281 ctgcgccacc tcgccggaaa gccgctccgt caaagcctga aaaagctgtg gtatccccgc
     8341 tccggtctgt gcggaaagcc agacacggat cggtttgttc tcttcgtccc gatcaatacg
     8401 cggttcgaaa tcttccagca tatcgatctt gttcatcacc agcagggttg ggatctcgtg
     8461 agcgtcgatc tcttcaagaa ccgtattcac cgcttcgatg ttttcttgta cacgcacatc
     8521 cgccgcatca atgacgtgca gcagtaatgt ggcttgccgc gtctcttgta acgtggcttt
     8581 aaatgctgca accagatcgt gcggcaggtg gcgaataaac cctacggtat ctgccaacac
     8641 ggtttcaccg acatctgcaa cgtcaatacg ccgcaacgtc gggtcgaggg tggcaaacaa
     8701 ctggtctgcc gcgtagaccc gcgcttcggt gatgcgattg aaaagggtag atttaccggc
     8761 gttggtatat cccaccagcg aaacagtagg aacgtcggct ttgatacgcg attgccgccc
     8821 ctgctcacgc tgcttttcaa ctctttccag gcgcgactgt atctgcacga tgcgattacg
     8881 caacaaacga cggtcggttt cgagctgggt ttcacccgga ccacgcaaac ctatcccgcc
     8941 tttctgtctt tcaaggtggg tccagccacg caccaggcgc gtagccagat ggcgcagctg
     9001 cgccagctca acctgcaact taccctcatg ggtacgcgca cgttgggcga aaatatctaa
     9061 aataaggccg gtgcggtcga taacacgaca ctcgcacaaa cgctccaggt tacgctcttg
     9121 cgccgggctc agggcatggt caaaaagaac gaccgaagca cccgtcgctt tgacagcttc
     9181 cgcaatttca actgctttac cttcacctac aaaatacttt gggtgcggcg ctttacggct
     9241 accggtaatc acctgcaatg cttcgacacc ggcggaagag accagagatt caaactcctg
     9301 gaggtcttcc atatctttgt cttgcgtaaa atagatgtgt accagtaccg cctgctcacc
     9361 agcatcataa cggtcaaaca agcgtataac cctctaaata gatcagcggg gaacgcagga
     9421 tcgctggctc cccgtgtaaa aaaaacagcc cgaaacctta ttcggtttct tcgctgtcct
     9481 gttgcgcgga agtattctgc gcgctgctac catgatggta gttactgctg gtaccgccac
     9541 cggcgttgtt actgtgatga gaaaccgggc gagacgggac aacagtagaa atcgcgtgct
     9601 tgtaaaccat ctggctgacc gtgtttttca gcaggatcac gaactgatca aaagactcga
     9661 tttgcccttg cagcttaata ccattcacca aataaataga aactggaaca cgttcccgac
     9721 gcagtgcgtt caggaacgga tcttgtaaag attgcccctt agccattctc tcttttcctt
     9781 atatgcttat ttgtactttg aacctttcga ttctgaaaaa attgcgcacg atacgtctca
     9841 attgtacaca ttcagcctgc gatagcacca acaacctgta atacttcgtc acgcgcctgt
     9901 tctggttttt cactatcaag ccagtgaacc ccttcccaac cacgcagcca ggttatctgc
     9961 cgcttcgcca actgtctcgt ggcgcaaaca cctcgataaa ccatttcatc gtatgagatt
    10021 tcgccttcaa ggtaagacca catctggcga taacccacgc aacgaatgga aggcaagtcc
    10081 gtatgcaaat ctcctcgggc aaaaagcgcc cggacttctg cttcaaaacc tgaagccaac
    10141 atctgatgaa aacgctgctc aatgcgttga tggagcagtt cacggctcgc cggggcgatg
    10201 gcgaactgat gcacctgata cggtagagcg tctcctgacg tttgcgtcag ttccgttaaa
    10261 gttttacccg aaatgaaaaa aacttccagt gcccgggaaa gcctttgtgg atcatttgga
    10321 tgaatccttg ctgccgcaac cggatctacc tcctgaagtt gacgatgcaa tgactcccaa
    10381 ccttgctctg ccgcctgttg ctcaattctg gcccgcactt ccgggtctgc cgacggtagc
    10441 ggcgacaacc cttccagcaa tgccttgaaa tacaacatcg taccgcccac taacagtggg
    10501 atccgccccg ccgcggtgat atcggccatt tccgccagcg catcgcggcg aaaatcagca
    10561 gccgagtaag cctgcgacgg atcgcgaata tccagcaatc ggtgcggcgc ggcgagtaac
    10621 tcttcagcgt tcggcttcgc cgtcccgata tccatccctt tgtaaataag ggcagaatca
    10681 acgcttatca actctactgg taaaatttta cgcagctcaa tggctaacgc cgttttaccg
    10741 gaggccgtcg gccccatcaa aaaaatcgcc ttaggcaggc tcgccttact gatatcactc
    10801 atctttcagg gcttttatcg ccggatgtaa atcaacagat tgtaacagac cacccggcgg
    10861 cgttttcaca agttgcggac ataaccgttc cacgtccgcc agcagggtta tggcctgtgc
    10921 cattgaccac tgcgcatgtt cgctcatcag atttcgtgca atccactgcg caatattgcc
    10981 aggttcgaat acggactgct tcgccaggta gcctatcagt tcaggaatca agatttgtaa
    11041 attttgttgg cgtaagggta aaggcacggc cctgatggtc acatgctgtg catctgactg
    11101 gaaatcaata cccaattccg ccagggcaga ctgcgctttt tctaatgccg atttttcttc
    11161 ggcagaaact tttagccgca acggaatcag cagcggctgg gcgcaaacgg gcgcttcacc
    11221 cggcgtcaat tgtgcctgac gtagccaacg ttctgccact ggcaaggata aaagtgaaat
    11281 gttgccatcg cgctccagca acgcacagtc ggaatggacg atagtcagta cccgaccaaa
    11341 actctgactg ttcgccgcaa gtgcaggttc ctgcggttcc ggcgcttttg gtttttgcat
    11401 cggcgcgggc gtttgcaaaa gctggcgata cacttcacct tgctgtttct ggtagcctgg
    11461 ctgcgcattc ggccaggggg cagccggacg actgcctgat gctggcgcag gagtgtagcg
    11521 cggagctacc ggctcacgag ctgccggttc tgcaaagtga ttgcgccccg ccgccacgcg
    11581 gttttccgga atggaacgcg gtgcaggttg gggttcatcg tccagcggta gcggcgtttc
    11641 cagttgctgt tgtagcacgc tcagcacgcc ctgatagata aaatcatgca ccagacgcga
    11701 ctgatggaaa cgcacttcgt gtttggcggg gtgcacgttg acgtccacct gatgcggatc
    11761 gatctccaga tacagcacaa atgccggttg ctgatcggcc cccagtttgt cttcgcaggc
    11821 ctggcggatc gcgtgattga tcaggcgatc gcgcatcatg cgaccgttca cgtagcaata
    11881 ctgaatttct gccagtgcgg gcgtggtgtg atttggatcg gccacccagc cgcgtagcgt
    11941 gagatcgccg tgttgccatt caatcgccag cgcttgttca agaaaagcgg tgccgcaaat
    12001 cgcgcctaag cgccgttctt tttgcccgcc ttccggcact gcgcggtact gacgcacaat
    12061 tttaccgtta tgcgacaggt tgatcgtgac gtcgaaacgc gccagcgcaa tgcggcggat
    12121 gatctcatca atgtggttaa attcggtttt ctcggtgcgc aggaatttgc gccgcgccgg
    12181 ggtgttgtag aacagatcca gcacctccag cgtcgtcccc acaggatgcg ccgccggttt
    12241 taccgtcacg ttcatatcgc gcccttcggc ataggcctgc caggcttcct gctgttctgc
    12301 ggtgcgtgaa gtgagcgtca ggcgggaaac cgaactgata ctcgccagcg cctcaccgcg
    12361 aaagcccagg ctgataatgg cttcgagatc gtccagagag gcgattttac tggtggcatg
    12421 acgcgccagc gccagcgcca gctcgtcttt tttgataccg cagccgttat cacgaatgcg
    12481 gataagtttc gccccaccgc gttcgatatc aatatcgata cgtgtcgcac ctgcatcgag
    12541 gctgttttcc accagttctt tgactaccga cgcaggtcgc tcgaccacct cacctgcggc
    12601 aatctggttc gccagttgtg gcggtaagac ctgaattggc atcaatcgtc cttagtttgg
    12661 cagcgtgcga tctggcgtcg tcaccgtact ggccgtttgt gccgttgccc cctgcggcgc
    12721 agattgcatc ggatgcgcaa ggaaataatt gcgcaggcct ttgtaaatgg cttctgccag
    12781 ctgttgttgg taatcgtcgc tcgccagcaa acgttcttcg ctgttgttgc tgataaaacc
    12841 ggtttcgacc agtactgatg ggatatccgg cgaacgcaga acgccgagac tcgcgtgttc
    12901 tggtcggcgt ttatgtattt cgccaatgcg ttgcaactga ctgatcatac tggtcgctac
    12961 atcataccct acccgttggg aatgaccgaa ctgtaaatcc agcaccgcct ggcttaaata
    13021 ggggtcagac tgactgttcg ccagcacatc acccgcccca cccagcagct ccgactgttt
    13081 ctcgtgctgc tccagccagc tggccatttc gctgttggcg cgacggttag agagcaccca
    13141 tacggaagcg ccagtcgcac tgcggttcgg tgcggcatca gcgtgaatcg acacgaggaa
    13201 attggcgttt tgcttacgtg ccacatcact gcgccccatc accgagataa agtaatcccc
    13261 gtcacgggtt aaaacgccct taaacatcgg atcgtcattg agcaaagtac gcaatttacg
    13321 cgcgatggcg atggtgacat ttttctcccg cgtaccaccg gggccgatag cgccagggtc
    13381 ctgaccgccg tgtccggcat caatagcgat gataatttta tcgccagtgt tagccgtcgc
    13441 gcgcgctgcc ggacgcgtta ccgtattact gctgataaca cccgtagtgc ggttactttc
    13501 cgttttaaac ggattgcgcg ccggttcgct gacgcgcggt gcgacaaccg caggcgtttc
    13561 aacgcgtttc gcaaccacgg gcggcggagg aggaggtggc ggcgcatcgg cgttaatcgt
    13621 aaagacgaca gtgtaattgc tgccattctg ccgcttcacc gcttcggttt taccgttttc
    13681 ggtaagatcg accaccagcc gtagcgtttg tgcatcttta ggcgttccag agcgaatcgc
    13741 cttcaccaga ttattgccgc tgaacaacaa cggcagtccc tgaatcacgc ccgtttgttt
    13801 gatatcgagc gccacggtgc gtttgctttg atggctaaac gcataatcag gatcgccaat
    13861 aaaactcaac gttatccgcg cctgttgatt accgttagaa acctgaatat cagagagcgt
    13921 cgcggcaccc accggcgtgc acagcagcag cagcgtcgct accaaccaat ttctgatgcg
    13981 atacatcatc ccgccacctt tcaaaggtta accggctaaa cgcgccagca acaattcacc
    14041 cgcagaggaa accgcactca cgcgcgcctc acggccttgt gcctgataat caatgtgtat
    14101 ttcgacatcc gggtcaggaa gaacaccagt accttgttgt ggccactcca ccaggcagat
    14161 ggcatcgttg gcaaaataat cgcgaatccc cataaactcc agctcctcgg gatcggcaag
    14221 gcggtacaaa tcaaagtgat aaaccattaa attgtcgagc gtatagggtt cgaccagcgt
    14281 ataagtgggg cttttgacat taccctgatg acccagagct tgtaaaaagc cccggctaaa
    14341 ggtggtttta cctgcgccta aatcgccata cagatagatt acggttgcgc catcgcaggc
    14401 tttcgctacc cgctcgccca ggtctaatgt tgcctgctca tcagggagcg gaattactcg
    14461 attcatcatg gtttttatca gtcacttccg ggttaacaat acgctgtagc gtggaaaaga
    14521 gatcggttgc cagcatcccg cgcgttccaa aacgcgccgc cagtacgtca gctgcagcac
    14581 cgtgcgcgac acagcccgca caggcggcat catacggcga cagtttttgc ccaagcaatg
    14641 cgccaataat accagagagc acatcgccca tgccgccgct cgccatgcct gcatttccgg
    14701 catcaataat gcctaaagcg tcaggatggg cggcgaccac ggttccggca cctttcagca
    14761 ccgctacgcc gccataacgt tgtaccagac gtttggcgca atgtaagcgg tcactttcaa
    14821 tttcagcgac ggaacagcct aacaaccgtg cggcctcgcc aggatgcggc gtgatcacgc
    14881 gattgtgacg cttatcggga ttgattgcca gcaggttcaa tgcatcggca tcccacaaca
    14941 tcggtttgcg aaaattctca actttttgca gtgctttttt cccccactct tgctggccca
    15001 gaccgggacc aatcaccacc acatcggccc attccaggct ttcggtaaga gagtccatcg
    15061 tcagttcatg caccatcaat tccggtcgtg cagtcagcag cggcgcaatg ttttcactgc
    15121 gggtcagtac tcggactaaa ccagcaccag cacgtagcgc cgcttccccc gtcatacgaa
    15181 tagcccccgc cgtgccgtga tcgccaccga taattaccag ccgcccgtga tcgcctttat
    15241 gcgaagtcgg gcgacgcggt tttagccagt gagaaagttg ttctgccgaa aaccgctgaa
    15301 ttttcgtctc ctgacctgcc agccaactat ccagccccag tgagtcaaaa tgcagttgtc
    15361 cggtaacatc ccgcgctttt ccagtgagca agcctggttt cagcgcaata aaagtgatgg
    15421 tgtgatcggc gttgatcact gcgcctggcg tagcgccggt ttcagccagc aggccggaag
    15481 ggatatcaac cgccgcaatc ggcgcaggat gggaattagc gtggtcgatt aactggctaa
    15541 tggattcgcg gggcgcttgc tgcaagccgg taccgagcag cgcatcaaca atcagatcta
    15601 ccgattcggg ccagacaata ttcgaagcat ggatctcgcc tcccgcgttt aaccatgctt
    15661 cgcgtgccag cgcggcctct tccggcaacg gtttgtcgct ctcctgggcc agcagcgtga
    15721 cctcaatgcc gaccgctgtg gccagccgcg cgaccacgta gccatcgccg ccgttattac
    15781 catgaccgca cagcaccagc caatggcggg cgtcaggata cgccgaacga cacacctgga
    15841 atgcggcttc gccagcgcga agcatcagct catagagtgt aagccccagc gcatctgccg
    15901 cctcacgttc tccgcggcgg atatcgtcgg cgtgccagac ggtgtgtggt atacttacgg
    15961 ggtttttctt cattgtatgg tccgtcatgt cagagcccct cgatctcaat cagttagcgc
    16021 aaaaaattaa acagtggggg ctggaactgg gctttcagca ggtaggtatt accgataccg
    16081 atctcagcga gtccgagccc aaactgcaag catggctgga caaacaatac cacggcgaaa
    16141 tggactggat ggcacgtcac ggtatgctgc gcgctcgccc ccatgagtta ttgcccggta
    16201 cgctgcgcgt gatcagcgtg cggatgaatt accttcctgc taacgccgca tttgccagca
    16261 cgctgaaaaa ccccaaactc ggctatgtta gccgttatgc gctgggccgt gactatcaca
    16321 aacttctgcg caaccgactc aaaaagctgg gcgagatgat tcagcaacat tgtgtttcgc
    16381 tgaattttag accgtttgtc gattctgcgc ctattctcga gcgcccgtta gctgaaaaag
    16441 ctgggctcgg ctggacaggt aagcactcac ttatcctcaa tcgcgaggcc ggttctttct
    16501 tctttttagg cgaattgctg gtcgatattc cgctgcccgt ggatcaacca gtcgaggaag
    16561 gatgcggtaa atgcgtggcc tgtatgacga tttgcccgac cggtgccatc gtcgagccat
    16621 ataccgtcga tgctcgccgc tgtatctctt atctcaccat cgaacttgaa ggggcgatcc
    16681 cggaagagtt gcgaccgtta atgggaaacc gtatttacgg ttgcgatgac tgccagctta
    16741 tctgcccgtg gaatcgctat tcacaactca ccacagaaga ggatttcagc ccgcgtaagc
    16801 cgctacacgc accggaactc attgagttat tcgcctggag cgaagagaag tttttaaaag
    16861 tcacggaagg atcggcgatt cgtcgtattg gtcacctgcg ttggctgcgt aatatcgccg
    16921 tagcattagg caatgcccct tgggatgaaa cgattttgac agcgctggaa agtcgtaaag
    16981 gtgagcaccc acttctcgat gagcacatag cgtgggcgat tgcgcagcaa atagagagac
    17041 gaaatgcgtg catagtcgaa gtgcaattgc cgaaaaaaca gcgtctggtt cgggtgattg
    17101 aaaaagggtt accgcgtgac gcctgattca tccacagctt gtgaataaaa ataaaaacgc
    17161 attgcaattc aagacgaata aaatcgtcaa gtgatcgact cgacaaaacg gaatcttaat
    17221 ttttaactta tctatcaata ggttaaaaaa atactattca ccgtgcgaag tttctttgaa
    17281 attaagatta cagcctgagg ctgtggataa ctctgtttac aagagtttgt cagagacaac
    17341 gaaaaccagc aataacgcat cgcatcgctg tggatatttt attgagagaa gaatttggag
    17401 cgggaaacga gactcgaact cgcgaccccg accttggcaa ggtcgtgctc taccaactga
    17461 gctattcccg cttgggtggt ctgtgctttg cagcactttt caaattttgg agcgggaaac
    17521 gagactcgaa ctcgcgaccc cgaccttggc aaggtcgtgc tctaccaact gagctattcc
    17581 cgcgtactac ttaattttgc tttcgtaatt ttcgcatttc tgcgtcgtta cgggaggcgc
    17641 attatacgag aaatctttcc ctctgcaacc ccccaaaaaa gttttttttg cgtttttatc
    17701 tcaagtgctg aaattatcag caagacgttc aatttagcgt caaaatcacg ccgccgcaag
    17761 tggattccgg cgcttacgtg accaggaaaa attttacagc ttgataaaat gctcgcggta
    17821 gtaagccagc tccgccaccg attcacggat atcatccatc gcctgatgcg tcccctgctt
    17881 ggtaaaacca tccagaattt ccggcttcca gcggcgcgcc agctctttca gggtgctgac
    17941 atcgagataa cggtagtgga agtaggcttc cagctccggc atgtatttaa acaggaaacg
    18001 acggtcctga ccgatgctgt taccgcaaat cggcgatttt cccgcaggca cccactgttt
    18061 taaaaattcg agcgttgcca gttcagcttc ccgatcgccc atcgtgctcg ctttcacgcg
    18121 ctctaccagc ccgctggcgg tatgggtgcg cacgttccag tcatccatca gcgccagctg
    18181 ttcatcagac tggtgtactg caatggtcgg cccttctgcc agaatattca ggttggcatc
    18241 ggtcaccagc gtggcaatct caataatgcg atcgcgctcg ggatccagac cggtcatctc
    18301 aagatcgatc caaatcaggt tgttttcatt ggcactcatg ctattttcca cccattcaca
    18361 gtgactatat tgatccacaa ttgcgtgtat catagatgtt ttgcccatca ggggcgacca
    18421 ggagtctgta cgattgagta aaaataaact ctccaaaggc cagcagcgcc gcgtgaacgc
    18481 caatcaccag cgtcgtctta aaacgtctaa ggagaagccc gactacgacg acaatctgtt
    18541 tggcgagcct gatgaaggta tcgtcatcag ccgctttggt atgcacgctg atgtggaatc
    18601 cgccgatggc gacgttcacc gctgcaatat tcgccgtacc atccgttcgc tggtaaccgg
    18661 cgaccgcgta gtctggcgtc cgggtaaacc ggcggcggaa ggcgtaaatg tcaaagggat
    18721 cgtggaagcg gtgcatgagc gtacctcggt gttgacgcgt ccggatttct acgacggcgt
    18781 aaaacctatt gccgccaaca tcgaccagat tgtcattgtc tccgccattt tgccggagct
    18841 gtcgctcaat attatcgacc gttacctggt ggcctgcgaa accttgcaga ttgagccgat
    18901 tattgtgctc aacaagatag acctgctgga cgacgaaggc atggcgttcg tcaacgagca
    18961 gatggatatc taccgcaata tcggttatcg cgtattgatg gtttccagcc atactcagga
    19021 tgggctaaaa ccgctggaag aggcgttgac cgggcgcatc agcatttttg ccgggcagtc
    19081 tggcgtcggc aaatccagcc tgctgaatgc gttgctgggg ctgcaaaaag agatcctgac
    19141 caacgatgtc tctgacaact cggggctcgg tcagcacacc acgaccgccg ctcggctgta
    19201 tcacttcccg cacggcggtg atgtgattga ttcccctggt gtgcgtgagt tcggcctctg
    19261 gcacctggag ccggaacaaa tcactcaggg ctttgtcgaa ttccatgact atttaggtct
    19321 gtgtaaatat cgcgattgca aacacgatac cgatccgggc tgcgctatcc gggaagcggt
    19381 tgaggaaggg aaaatcgcgg aaacccgttt cgaaaactat caccgtattc tggaaagcat
    19441 ggcgcaggta aaaacgcgta aaaacttttc tgatacggat gactgacaat taagctaacc
    19501 gtcgttagaa tcgtcccctt tttttcagga tccggcatgt tggccggatc aggaacgaca
    19561 aaacaatggc ctggaggcta ccttgttaaa ttcatttaaa ctttcgctac agtacattct
    19621 gccgaaacta tggcttactc gcctggcggg ttggggcgca agcaagcggg caggatggct
    19681 gacaaaactg gttatcgatc tgttcgttaa atactacaag gtcgacatga aagaggcgca
    19741 aaagccggac accgccagct accgcacctt taacgaattc tttgtccgtc cgctgcgtga
    19801 cgaagtacgc ccaatcgata ccgatccgaa tgtactggtc atgcctgccg atggcgttat
    19861 cagccagttg ggtaaaatcg aagaagataa aatcctgcaa gccaaaggcc acaactacag
    19921 cctcgaagcc ctgctggcag gcaactatct gatggcggac ctgttccgca acggtacgtt
    19981 tgtgaccact tacctctccc cgcgtgacta ccatcgcgta cacatgccgt gcaacggtat
    20041 tctgcgtgag atgatctacg taccgggcga tctcttctcc gttaaccatc tcacggctca
    20101 gaacgtgccg aatctgtttg cccgtaacga acgcgtgatt tgccttttcg ataccgaatt
    20161 tggcccaatg gcgcagattc tggtcggagc gacgattgtt ggcagcattg agacggtctg
    20221 ggcgggcacc attacgccgc cgcgcgaagg tatcatcaag cgctggacct ggcctgccgg
    20281 ggaaaacgac ggttctgtgg cactgctgaa aggccaggaa atgggtcgct ttaaactcgg
    20341 ttccaccgtt atcaacctgt ttgcaccggg taaagtgaat ctggttgagc aactggaaag
    20401 cctgtctgtc acgaaaattg gccagccgct ggcagtatct accgaaacct ttgttacgcc
    20461 agacgctgaa ccggccccgc ttcctgctga agagatcgaa gcagaacacg acgccagccc
    20521 attggttgac gataaaaaag accaggtcta atccatcaaa ggaaacgctg acgtgcgcct
    20581 gattatcact tttctgatgg cctggtgcct cagttggggg gcgtacgccg cgacggcccc
    20641 cgatagcaaa caaatctcac aagaactgga gcaggcaaaa gcggcgaaac ccgcgcagcc
    20701 ggaagtcgta gaggcgctcc agtccgcctt aaatgcgctt gaggaacgaa aaggttccct
    20761 tgagcgcatc aagcaatatc aagaagtcat tgataattac ccgaaacttt ccgctactct
    20821 gcgcgcacaa ttaaacaaca tgcgtgacga accgcgcagc gtgtcgccgg ggatgtctac
    20881 cgacgcgctg aatcaggaaa ttctccaggt cagcagccag ttgctggata aaagccgtca
    20941 ggcccagcaa gagcaggagc gcgcccgcga gattgccgat tcgctgaatc aactgccgca
    21001 acagcaaacc gacgcccgcc gtcagttaaa tgagatcgag cgccgcctgg gaacgcttac
    21061 tggcaatacc ccgctcaatc aggcacaaaa tttcgcgttg cagtctgact ccgcacgcct
    21121 gaaagcgctc gttgatgaac tggaactggc gcagctgtct gccaataacc gtcaggaatt
    21181 agcgcgctta cgctcagagc tggcggaaaa agagagccag caactggatg cgtatttgca
    21241 ggccttacgt aatcagttga acagccaacg tcagcttgag gcggagcggg cgctggaaag
    21301 taccgaattg ctggcagaaa acagcgccga tttgccgaaa gacatcgtcg cgcaattcaa
    21361 aattaaccgc gaactatcgg cggcactgaa tcaacaggcg cagcggatgg atctcgttgc
    21421 ctcgcaacag cgccaggcca ccagccagac gttacaggtt cggcaggcgc tgaatacgct
    21481 gcgtgaacag tcgcaatggc tgggatcgtc caacctgctc ggcgaagcgt tgcgggcgca
    21541 ggtggcacgg ctgccggaaa tgccgaaacc acaacagctt gataccgaaa tggcgcagtt
    21601 gcgtgtgcaa cggttacgtt atgaggatct gcttaataaa cagccgctgc tacggcaaat
    21661 tcatcaggcc gacggtcagc cgctgaccgc cgagcaaaac cgtattctgg aagcacagct
    21721 acgcactcag cgtgagttgc tgaactcatt gttgcagggt ggcgacacgc tactgctgga
    21781 actgaccaag ctgaaagtct ccaacgggca acttgaggat gcgctgaaag aggtgaatga
    21841 agcgacgcat cgctatctgt tctggacctc tgacgtgcgc ccgatgacca tcgcctggcc
    21901 actggaaatc gcccaggatc tgcgtcgtct catttcgctg gacaccttca gtcagttggg
    21961 caaagccagt gtgatgatgc tgaccagcaa agagacgatt ttgccgctgt ttggcgcgtt
    22021 gattctggtc ggttgcagta tttactcgcg ccgctatttc acccgttttc ttgaacgttc
    22081 ggcggcgaaa gtcggcaaag tgactcagga tcacttctgg ctgacgttgc gcactctttt
    22141 ctggtcgatt ctcgtcgcgt caccgctgcc ggtgctgtgg atgacgctgg gttacggctt
    22201 gcgcgaggcg tggccttatc cgctggcggt cgcgattggc gatggcgtaa cggccaccgt
    22261 gccgctgctg tgggtagtga tgatttgcgc cacctttgcc cgcccgaacg gcttgtttat
    22321 cgctcatttt ggctggccgc gcgaacgtgt ttcccgtggg atgcgctact acctgatgag
    22381 catcgggctt attgtgccgc tgattatggc gctgatgatg ttcgataacc tcgacgaccg
    22441 tgaattctcc ggttcgctgg gacggctttg ctttatcctc atttgcggtg cgctggcggt
    22501 ggtcaccctc agcctgaaaa aggccgggat cccactgtat ctcaacaaag agggcagcgg
    22561 cgacaacatt accaaccata tgctgtggaa catgatgatt ggcgcgccgt tggttgccat
    22621 tctggcgtcg gctgtgggtt atctggcaac ggcacaggcg ctgttagcga ggcttgaaac
    22681 ctcggttgcc atctggttcc tgctactggt ggtttatcac gttatccgcc gctggatgct
    22741 gatccagcgt cgcaggctgg cgtttgaccg ggcgaagcat cgccgggcag agatgttagc
    22801 gcaacgcgcg cgtggcgaag aggaagcgca tcatcacagt agcccggaag gggcaattga
    22861 agtcgatgaa agcgaagtcg atctcgatgc catcagtgcg caatccttgc ggctggtgcg
    22921 ctcaattttg atgttgatcg ccctgctttc ggtcattgtg ctgtggtcag aaatccattc
    22981 cgctttcggc ttcctcgaaa atatttctct gtgggatgtc acttccactg tacagggcgt
    23041 agaaagcctg gagccgatta ccctcggtgc agtgctaatt gccattctgg tatttatcat
    23101 caccacgcag ctggtgcgca acctgccagc gctgctggaa ctggcgattt tgcagcacct
    23161 ggatttaact ccgggtacgg gttacgccat caccaccatc accaaatatc tgctgatgct
    23221 gattggcggg ctggtcggct tctcaatgat tggtattgag tggtcgaaat tgcagtggct
    23281 ggttgccgcg ctcggtgttg gtctcggttt tggtttgcag gaaattttcg ccaactttat
    23341 ctctggtctg attatcctgt tcgaaaaacc gattcgcatt ggcgatacgg tgacaattcg
    23401 cgatctcacc ggtagcgtga cgaaaattaa cacccgcgcc accaccatca gcgactggga
    23461 ccgtaaagag ataatcgtgc cgaacaaggc gtttattacc gagcagttta tcaactggtc
    23521 gctctctgac tcggtcacgc gcgtggtgtt gacgataccg gcccctgccg atgccaatag
    23581 cgaagaagtg acggaaatcc tgctcaccgc agcgcgtcgc tgctcgctgg tgatcgacaa
    23641 cccggcaccg gaagtcttcc tggtggatct gcaacagggg attcagattt tcgagctgcg
    23701 tatttacgcc gctgagatgg gtcaccgtat gccgctacgc catgagatcc accagctgat
    23761 tctggcaggc ttccacgccc acggtatcga tatgccattc ccgcccttcc agatgcgtct
    23821 ggaaagcctc aacggtaaac aaacggggag aacgctgacg tctgcgggca aaggtcgtca
    23881 ggcgggaagt ttgtaagttt gtgtgccgga tgaaccaatc cggctcacaa acaaaactga
    23941 tagcgcagaa aaaactgcca cagccagtag ccgaaaatag ccagcgcggt ggcgtacata
    24001 aaccagccgc gtttttttcg ccacaaagcg aaccagagaa aagggaaaaa gagcggtaac
    24061 gtcattatca caccaacatc acgggtatcg tcttcttcga gatacatcaa cacatcgcag
    24121 acgttgttaa tcccgcgtcc atcaatcatc cactctttgt ccagcgcgga agtgatggtt
    24181 atccagagga acgccacaat aaaccagatg cagcacaaga caaacatgcg cgcactcata
    24241 aggtaaccgg tcgcttaatt tcgctatagc gcatacgaaa caaatcgccg tatcccacta
    24301 attccccgcg aaaaaccaga catcggataa gataagcgca ataaatatcc ggcaggtttc
    24361 cccaccgttt accatcgcga tctttgaggg tgttaatggt gctacccacc accatcgcca
    24421 ctttacgggt gtggcaggca tcgacaaaag agagaatcgt ctgctctatt tccgctacat
    24481 ccccggcaga aaattgcagc gccctgattt catcttcggt tattgccggg tcccggctgg
    24541 tgtcatccat aacatttcct gtctttacgc cctatcgtca ggcagacgat agggcatcgg
    24601 gttattccac cgcccactgc gggttttttg ccactctgct gcaatagttc tggtaaatcg
    24661 ccatcgctaa cagcgagaag aagacagggc cgccaatcat ccacaatgtg ctgtcccagt
    24721 ctccggcttc aaccacaggc tgaataatgg tgaagacgtt cgcaaatgtc accaccagta
    24781 cgacaaccac tgtcgcaatc attgccgaca aatgcgtttt gaaaatcaca aatggtctgt
    24841 cgagatcctg acgtgcttta aagaatggga aagccagcgc gaggaacagg taaggaagcg
    24901 tcatagacac gttcgccatc agcgtcagct tgttaaagaa cgcggatgcg gtaccgccac
    24961 caaacgaaac cagcaggatg aagacagtaa ccaacccgca ctgcatccac atggcgatag
    25021 acggcatccc catcgcattc aggcgcgtca tcggttccgg ccacaacgct ttcggcgtcc
    25081 cctggatgat ggctttcagc ggtgaatagc aaagcgtaaa gaacgcaccg gtataagcga
    25141 ggaacatcga aagtccggta atacgcgcaa accatacgcc cagcgacaat gacgcttcag
    25201 gtgacaaatg cagtgcgtta cccagcgtca ccccgaggct cttcatcagc acataggtaa
    25261 tattgccaag gttaacggaa ccattactta atacctgctg ccagtttgtg ctgacacccc
    25321 ataaaaatat tgccagcgaa taaccgattg aaataacaat agcggcaaaa acaatacctt
    25381 tggcaaagtt cttttctgga ttttccgttt tatcgaccag accaccaacc gcttcaattc
    25441 cgccataggc aaaaatagca aataccacaa acgatagcat tgccagaccg gactgataac
    25501 ctgggttcgg tgatgcaagg aaattaatat cctgcgcgaa atgcccacca tttaataaca
    25561 aaatagtaat gcttactaac agcaatacta aattcagaca cattactgca ataccgccca
    25621 ccgcagtaat gcgggcaatt ttattaatcc cctttgaagc aacgacggtg accagaatca
    25681 tccatgccac tgccagcaga ccaaccacct gcgtaggctc cagtccagca atacgccagt
    25741 gctgggtcat gtcgctacca tagaggaatg ttgagaacgg tacccaaact ttcgcggagg
    25801 tactcaccat ccagatgata taagaggaaa accacataaa cgtaccaatg aaggcaaaac
    25861 gtggtccgac actattattc atccaggaat agataccgcc ttcttctttg cgataagcag
    25921 ctcccatttc agccatcatt aaggcgaatg gaataaagaa taacaatgca gaaaatatat
    25981 aaaagggaat cgcactataa cccattaagt aataagccga tgggctattg gcaaatccaa
    26041 atacggaagt aaagatcatc aatatgagtc ctatcagact catctttttt atcgtgtgag
    26101 gcatgaaacc atccttcatt tcagcgcgtg tataacacgt gcccactgaa ctgcactgcg
    26161 caacattgag caggcagcgt aaaaaccaat acagaattga tacctggatg atatcaaata
    26221 tccagtctta aattgtggcc tttaacggaa aaatgaaaac atatgctaca gaatggcgct
    26281 tatcacgcca ttcttcgctg ttaattcagt aatttttcag aattatgccc ggtcaacgct
    26341 aaaggcgatg acttcagcca gtgtctccgc gcccagcgcc aacatcacca gacgatcaac
    26401 acctaatgcc acgccggaac agtcaggcat accgactttc aaggcttcaa tcagattctg
    26461 gtcaatgggg tgctgcggca aaccgcgcgc cgcgcgctta cggttatctt gttcaaagcg
    26521 ttgttgctgc tcacgggcat ccgtcaattc atggaaacca ttcgccagct caataccttt
    26581 ataataaacc tcaaagcgtt cagcgacccg atgatcttcg gtactgattt gcgccagtga
    26641 tgcctggctg gctggaaagt ggtacacaaa ggtcggtttt tctttgccaa tatttggctc
    26701 tacgccaaag gtaaacagca attgtagcag cgtgtcgcgg tcttcttcgg tatcagcaac
    26761 attgctcaaa tccagtttcg ctgcgacttc ccgcagttgc gttttgtcgg cagagagcgg
    26821 gtcaatttcc agataacgca agaaagcttg ttgataagaa aggctttctg ctgccgggca
    26881 gtccagcacc tgttgtaaga gatcgtccac ctcgttcatc aaccggtaca tatcatagtg
    26941 cggtcgatac cactccagca tagtgaactc agggttgtga taacgcccca tctcttcatt
    27001 acggaagctg cggcacagct ggaataccgg cccacaaccg gcaaccagca ggcgtttcat
    27061 atggtattcc gggctggtca ttaaccagag attcatcccc tgcgaatgcc cggggccaac
    27121 gaaacgtgtc tcaaacggga ccaaatgaat atcggttacc gtcgcctggc tcatacaagg
    27181 cgtctccacc tccagcactc cacgatcggc aaagaaacga cggatctccg ccataatcgc
    27241 cgcgcgtttt aataagttag gaatggatgc gctcggctgc caggatgccg tttcgctcat
    27301 agttaaatct ccagtttttg acaagggcac gaagtctact cgcaacgcga cggcgagaca
    27361 aattttacgc aggaatcaaa cagcggtggg cagtgactaa aaaaagcacg atctgatggt
    27421 ttagtaatta aattaatcat cttcagtgat aatttagccc tcttgcgcac taaaaaaatc
    27481 gatctcgtca aatttcagac ttatccatca gactatactg ttgtacctat aaaggagcag
    27541 tggaatagcg ttcgcagacc gtaactttca ggcacttacc ctgaagtacg gggctgtggg
    27601 ataaaaacaa tctggaggaa tgtcgtgcaa acctttcaag ccgatcttgc cattgtaggc
    27661 gccggtggcg cgggattacg tgctgcaatt gctgccgcgc aggcaaatcc aaatgcaaaa
    27721 atcgcactaa tctcaaaagt atacccgatg cgtagccata ccgttgctgc agaagggggc
    27781 tccgccgctg tcgcgcagga tcatgacagc ttcgaatatc actttcacga tacagtagcg
    27841 ggtggcgact ggttgtgtga gcaggatgtc gtggattatt tcgtccacca ctgcccaacc
    27901 gaaatgaccc aactggaact gtgggggtgc ccatggagcc gtcgcccgga tggtagcgtc
    27961 aacgtacgtc gcttcggcgg catgaaaatc gagcgcacct ggttcgccgc cgataagacc
    28021 ggcttccata tgctgcacac gctgttccag acctctctgc aattcccgca gatccagcgt
    28081 tttgacgaac atttcgtgct ggatattctg gttgatgatg gtcatgttcg cggcctggta
    28141 gcaatgaaca tgatggaagg cacgctggtg cagatccgtg ctaacgcggt cgttatggct
    28201 actggcggtg cgggtcgcgt ttatcgttac aacaccaacg gcggcatcgt taccggtgac
    28261 ggtatgggta tggcgctaag ccacggcgtt ccgctgcgtg acatggaatt cgttcagtat
    28321 cacccaaccg gtctgccagg ttccggtatc ctgatgaccg aaggctgccg cggtgaaggc
    28381 ggtattctgg tcaacaaaaa tggctaccgt tatctgcaag attacggcat gggcccggaa
    28441 actccgctgg gcgagccgaa aaacaaatat atggaactgg gtccacgcga caaagtttct
    28501 caggccttct ggcacgaatg gcgtaaaggc aacaccatct ccacgccgcg tggcgatgtg
    28561 gtttatctcg acctgcgtca cctcggcgag aaaaaactgc atgaacgtct gccgttcatc
    28621 tgcgaactgg cgaaagcgta cgttggcgtc gatccggtta aagaaccgat tccggtacgt
    28681 ccgaccgcac actacaccat gggcggtatc gaaaccgatc agaactgtga aacccgcatt
    28741 aaaggtctgt tcgccgtggg tgaatgttcc tctgttggtc tgcacggtgc aaaccgtctg
    28801 ggctccaact ccctggcgga actggtggtc ttcggccgtc tggccggtga acaagcgaca
    28861 gagcgtgcag caactgccgg taatggcaac gaagcggcaa ttgaagcgca ggcagctggc
    28921 gttgaacaac gtctgaaaga tctggttaac caggatggcg gcgaaaactg ggcgaagatc
    28981 cgcgacgaaa tgggcctggc aatggaagaa ggttgcggta tctaccgtac gccggaactg
    29041 atgcagaaaa ccatcgacaa gctggcagag ctgcaggaac gcttcaagcg cgtgcgcatc
    29101 accgacacct ccagcgtgtt caacaccgac ctgctctaca ccattgaact gggccacggt
    29161 ctgaacgttg ctgaatgtat ggcgcactcc gcaatggcac gtaaagagtc ccgcggcgca
    29221 caccagcgtc tggacgaagg ttgcaccgag cgtgacgacg tcaacttcct caaacacacc
    29281 ctcgccttcc gcgatgctga tggcacgact cgcctggagt acagcgacgt gaagattact
    29341 acgctgccgc cagctaaacg tgtttacggt ggcgaagcgg atgcagccga taaggcggaa
    29401 gcagccaata agaaggagaa ggcgaatggc tgagatgaaa aacctaaaaa ttgaggtggt
    29461 gcgctataac ccggaagtcg ataccgcacc gcatagcgca ttctatgaag tgccttatga
    29521 cgcaactacc tcattactgg atgcgctggg ctacatcaaa gacaacctgg caccggacct
    29581 gagttaccgc tggtcctgcc gtatggcgat ttgtggttcc tgcggcatga tggttaacaa
    29641 cgtgccaaaa ctggcatgta aaaccttcct gcgtgattac accgacggta tgaaggttga
    29701 agcgttagct aacttcccga ttgaacgcga tctggtggtc gatatgaccc acttcatcga
    29761 aagtctggaa gcgatcaaac cgtacatcat cggcaactcc cgcaccgcgg atcagggtac
    29821 taacatccag accccggcgc agatggcaaa gtatcaccag ttctccggtt gcatcaactg
    29881 tggtctgtgc tacgccgcgt gcccgcagtt tggcctgaac ccagagttca tcggtccggc
    29941 tgccattacg ctggcgcatc gttataacga agatagccgc gaccacggta agaaggagcg
    30001 tatggcgcag ttgaacagcc agaacggcgt atggagctgt actttcgtgg gctattgctc
    30061 cgaagtctgc ccgaaacacg tcgatccggc tgcggccatt cagcagggca aagtagaaag
    30121 ttcgaaagac tttcttatcg cgaccctgaa accacgctaa ggagtgcaac atgacgacta
    30181 aacgtaaacc gtatgtacgg ccaatgacgt ccacctggtg gaaaaaattg ccgttttatc
    30241 gcttttacat gctgcgcgaa ggcacggcgg ttccggctgt gtggttcagc attgaactga
    30301 ttttcgggct gtttgccctg aaaaatggcc cggaagcctg ggcgggattc gtcgactttt
    30361 tacaaaaccc ggttatcgtg atcattaacc tgatcactct ggcggcagct ctgctgcaca
    30421 ccaaaacctg gtttgaactg gcaccgaaag cggccaatat cattgtaaaa gacgaaaaaa
    30481 tgggaccaga gccaattatc aaaagtctct gggcggtaac tgtggttgcc accatcgtaa
    30541 tcctgtttgt tgccctgtac tggtaaggag cctgagatga ttaatccaaa tccaaagcgt
    30601 tctgacgaac cggtattctg gggcctcttc ggggccggtg gtatgtggag cgcaattatt
    30661 gcgccggtga tgatcctgct ggtgggtatt ctgctgccac tggggctgtt tccgggtgat
    30721 gcgctgagct acgagcgcgt tctggcgttc gcgcagagct tcattggtcg cgtattcctg
    30781 ttcctgatga tcgttctgcc gctgtggtgt ggtttacacc gtatgcacca cgcgatgcac
    30841 gatctgaaaa ttcacgtgcc tgcgggcaaa tgggttttct acggtctggc tgctatcctg
    30901 acagttgtca cgctgattgg tatcgttaca atctaacgta tcgccaatgt aaatccggcc
    30961 cgcctatggc gggccgtttt gtatggaaac cagaccttat gttcaaaacg acgctctgcg
    31021 ccttattaat taccgcctct tgctccacat ttgctgcccc tcaacaaatc aacgatattg
    31081 tgcatcgcac aattaccccg cttatagagc aacaaaagat ccccggtatg gcggtggcgg
    31141 taatttatca gggtaaacct tattacttta cctggggcta tgcggacatc gccaaaaagc
    31201 agcccgtcac acagcaaacg ttgtttgagt taggttcggt cagcaaaaca tttacgggcg
    31261 tgcttggtgg cgaggctatt gctcgagggg aaatcaagtt aagcgatccc acaacaaaat
    31321 actggcctga acttaccgct aaacagtgga atgggatcac actattacat ctcgcaacct
    31381 acactgctgg cggcctgcca ttgcaggtgc cggatgaggt gaaatcctca agcgacttgc
    31441 tgcgcttcta tcaaaactgg cagcctgcat gggcgccagg aacacaacgt ctgtatgcca
    31501 actccagtat cggtttgttc ggcgcactgg ctgtgaagcc gtctggtttg agttttgagc
    31561 aggcgatgca aacacgtgtc ttccagccac tcaaactcaa ccatacgtgg attaatgtac
    31621 cgcccgcaga agaaaagaat tacgcctggg gatatcgcga aggcaaggca gttcatgttt
    31681 cgccaggggc gttagatgct gaaacttatg gtgtgaagtc gaccattgaa gatatggcct
    31741 gctgggtacg aagcaatatg aatccccgtg atatcaacga caaaacactt cagcaaggga
    31801 tacaactggc acaatctcgc tactggcaaa ccggcgatat gtatcagggc ctgggctggg
    31861 aaatgctgga ctggccggta aatcctgaca gcatcattaa cggcagtggc aataaaattg
    31921 cactggcagc acaccccgta aaagcgatta cgcccccaac tcctgcagta cgcgcatcat
    31981 gggtacataa aacaggggca accggcggat ttggtagcta tgtcgcgttt attccagaaa
    32041 aagagctggg tatagtgatg ctggctaaca aaaactatcc caatccagcg agagtcgccg
    32101 ccgcctggca gattctcaac gctctacagt aaaatttcat cgggtccgaa ttttcggacc
    32161 ttttctgcgc ttttccttgc tgtcatctac acttagaaaa aaaccagtaa ggaaacattt
    32221 atgcgcctgc tccctctcgt tgccgcagcg acagctgcat ttctggtcgt tgcctgcagt
    32281 tctcctacgc cgccgcgtgg cgtaacagta gtgaataatt tcgacgccaa acgttatctc
    32341 ggtacctggt atgagattgc ccgttttgat caccgttttg aacgtggact ggaaaaagtc
    32401 accgcaacat acagcctgcg tgatgacggc ggcctgaatg tcattaataa aggctataac
    32461 cctgacagag gaatgtggca gcagagtgaa gggaaagcgt actttaccgg cgcaccaact
    32521 cgcgctgcgc tgaaagtgtc attctttggt cctttctatg gcggttataa cgttattgca
    32581 ctcgatcggg aataccgcca tgcgctggtt tgcggcccgg accgcgacta cctgtggata
    32641 ctctcccgca cgccaaccat ttctgacgaa gtgaaacagg agatgctggc agtcgcgacc
    32701 cgggaagggt ttgatgtcag taagtttatt tgggtacagc agcccggtag ttagtgagtg
    32761 ctgagtttca gaccaataat ccccaatacg attaacgcca gactcgccag gcgcatcggg
    32821 ttagcggact caccgagcag cacaatgccg gtgatagccg cgccgactgc gccaataccc
    32881 gtccacacgg cataagccgt ccctactggt aacgatttca tcgcccaggc aagtagcgcc
    32941 atactgacaa tcatcgccgt cacggtaata acactcggcg tcaaacgact aaagccgtgg
    33001 gtatatttca ggccaacggc ccataccact tccagcagac cagcaataac taagataatc
    33061 caggacatat caggctccgg agaattgggg ccgtccccgg tgaaaagagg cgtttgcagg
    33121 tcgtcctgca aagctgatgg ttgaacggat attttgccag gtggcagggg aatttcaact
    33181 attaccttag tgactatcgc agaaaaaaca aacggcacga catacctgtc gtaccgttat
    33241 tgcttattgc tattgctgcg ctttcgttgc tgcgccagaa atcgcgttac cgccatcaga
    33301 aatgtcttca ccaacgccac gcgtggtgtt gcaggcagtt aatactgttg aaagcaccag
    33361 aacagaaaag atcgctgcaa ttgtcttctt caccataacg tcttcctttt aggtaatgtt
    33421 atttatgttt gcctatagca aattaataat agacaacatc gccaaaaatg acggaaatct
    33481 gatgattttt cggaagagaa gaaaaattag ctggcagcgc gagagatgga gttgccgaga
    33541 tgtttgatgt cttcgccgaa accgcgagcg gtgttacagc ccgtgagcag cgtgctggca
    33601 agcaaaacaa gaacgataag gcgtttcatc atctctgacc tgaataagtg atggtgcagc
    33661 ctgcaggccg caccacaacc gcattacttc acgcgagaga cgtattcacc agagcgggta
    33721 tccactttga tgacttcgcc gatttgtacg aacagcggaa ccttaaccac agcgccagta
    33781 gacagggtag ccggtttgcc gccagtacct gcggtatcac ctttcaggcc cggatcggta
    33841 tcaacgattt ccagttcaac gaagttcggc ggagtaacgg agatcggctg accattccac
    33901 agagttacga tacactctgc ctgatccagc agccatttag cgttgtcgcc aattgctttt
    33961 gcatcagcag acagctgctc gaaagtttcg ttgttcatga agtgccagaa ctcaccgtcg
    34021 ttgtacaggt aagtcaggtt catatcgaca acatcagcgc cttcagcgga atcagtagat
    34081 ttgaaggttt tttctacgcg agtaccggtc agcagacgac gcagtttaac gcgagcaaat
    34141 gcctggcctt tacccggttt tacgaattca ctcgcttcaa ccgcgtaagg ttcgccgtct
    34201 aacatgattt taagaccagc acgaaaatcg ttgctatagt acgttgccat aaggccctct
    34261 gaaatttgtt aattggtagc taagccacaa aatggcgcat attgtaaccc taaatacccc
    34321 atccagagaa gattggttaa cgcaacttgc cgatgttgtg accgatcctg atgaacttct
    34381 gcgtcttttg aatatagacg cggacgaaaa actgttggcc ggacgcagcg ccaaaaagct
    34441 gtttgccctg cgtgtgcccc gctcatttat cgatcgcatg gagaaaggca atccggacga
    34501 tcctcttttg catcaggtac ttacctcgca agatgagttt gtcgtcgcgt ccggattctc
    34561 caccgacccg ctggaagaac agcacagcgt agtgcctggt ttgttgcata aataccacaa
    34621 ccgggcgctt ttgctggtca aaggcggctg cgcggtaaat tgccgctatt gcttccgtcg
    34681 ccacttcccc tatgccgaaa atcagggcaa caagcgtaac tggcagactg ctcttgagta
    34741 tgttgctgcg catccggaac tggacgagat gattttctcc ggcggcgatc cgctgatggc
    34801 gaaagatcac gagctggact ggttgctcac acaactggaa gccatcccgc atatcaaacg
    34861 tctgcggatt cacagccgtc tgccgattgt gatcccggca cgtatcaccg aggcgctggt
    34921 tgaacgcttt gcccgttcta cgctgcaaat cttgctggtg aatcacatca atcatgccaa
    34981 tgaggtagat gaaacgttcc gtcaggcgat ggctaaattg cgccgtgtcg gtgtcaccct
    35041 gcttaaccag agcgttctgt tacgtggtgt gaacgataac gcacaaacgc tggcaaacct
    35101 gagtaatgcg ttgttcgatg ccggcgtaat gccgtattac ctgcatgtgc tcgataaagt
    35161 acagggcgcg gcgcatttta tggtgagtga tgacgaagca cggcagatta tgcgtgagtt
    35221 gctgacactg gtgtcgggtt atctggtgcc gaaactggcg cgagaaatcg gcggcgaacc
    35281 cagcaaaacg ccgctggatc tccagctacg ccagcagtaa ctgattaaac agggaagtta
    35341 aacattagcc tgatgcgcta cgcttatcag gcctacattg tgcattgaaa ttgaatggat
    35401 ttgcaatgaa ctgtaggccg gataaggcgt ttacgccgca tccggcaaga gcaacaaacg
    35461 caagtacagc aacctgtcat taaactggga aatttaatct ttgcccgttg cataaccttt
    35521 aattattcat atacattgct gttgttactc tttatatagc caacaccaat aattaacctt
    35581 tcttttctgt ttatatagag tgagtacggc tatttatgtt attttaacaa ctattactac
    35641 caagcccatt tatctg
//
LOCUS       DAPHDW010000035        34770 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.035, whole genome shotgun sequence.
ACCESSION   DAPHDW010000035 DAPHDW010000000
VERSION     DAPHDW010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 34770)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34770)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34770
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.035"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..964)
                     /locus_tag="RH057_001697"
     CDS             complement(<1..964)
                     /locus_tag="RH057_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element IS621 family transposase"
                     /protein_id="HDV3128081.1"
                     /translation="MEHELHYIGIDTAKEKLDVDVLRPDGRHRTKKFANTTKGHDELV
                     SWLKGHKIDHAHICIEATGTYMEPVAECLYDAGYIVSVINPALGKAFAQSEGLRNKTD
                     TVDARMLAEFCRQKRPAAWEAPHPLERALRALVVRHQALTDMHTQELNRTETAREVQR
                     PSIDAHLLWLEAELKRLEKQIKDLTDDDPDMKHRRKLLESIPGIGEKTSAVLLAYIGL
                     KDRFAHARQFAAFAGLTPRRYESGSSVRGASRMSKAGHVSLRRALYMPAMVATSKTEW
                     GRAFRDRLAANGKKGKVILGAMMRKLAQVAYGVLKSGVPFDASRH"
     gene            complement(1212..1787)
                     /gene="nfuA"
                     /locus_tag="RH057_001698"
     CDS             complement(1212..1787)
                     /gene="nfuA"
                     /locus_tag="RH057_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HDV3128082.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            complement(1846..2529)
                     /gene="gntX"
                     /locus_tag="RH057_001699"
     CDS             complement(1846..2529)
                     /gene="gntX"
                     /locus_tag="RH057_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HDV3128083.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIVSVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAAAV
                     QVWCLCRTL"
     gene            2567..3337
                     /gene="bioH"
                     /locus_tag="RH057_001700"
     CDS             2567..3337
                     /gene="bioH"
                     /locus_tag="RH057_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HDV3128084.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRV"
     gene            complement(3366..4244)
                     /gene="rpnA"
                     /locus_tag="RH057_001701"
                     /pseudo
     CDS             complement(3366..4244)
                     /gene="rpnA"
                     /locus_tag="RH057_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417870.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
     gene            complement(4447..4683)
                     /gene="feoC"
                     /locus_tag="RH057_001702"
     CDS             complement(4447..4683)
                     /gene="feoC"
                     /locus_tag="RH057_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HDV3128085.1"
                     /translation="MASLIQVRDLLALRGRMEAAQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            complement(4683..7004)
                     /gene="feoB"
                     /locus_tag="RH057_001703"
     CDS             complement(4683..7004)
                     /gene="feoB"
                     /locus_tag="RH057_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HDV3128086.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDAALARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFDVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAIDETWQSL
                     KDTFSLSVLMNPIEASKGDGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVVIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            complement(7021..7248)
                     /gene="feoA"
                     /locus_tag="RH057_001704"
     CDS             complement(7021..7248)
                     /gene="feoA"
                     /locus_tag="RH057_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HDV3128087.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFNVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            complement(7686..10007)
                     /locus_tag="RH057_001705"
     CDS             complement(7686..10007)
                     /locus_tag="RH057_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HDV3128088.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDAALLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     GVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNARRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            complement(10104..10580)
                     /gene="greB"
                     /locus_tag="RH057_001706"
     CDS             complement(10104..10580)
                     /gene="greB"
                     /locus_tag="RH057_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HDV3128089.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEASWYVNAIEYVKP"
     gene            10808..11527
                     /gene="ompR"
                     /locus_tag="RH057_001707"
     CDS             10808..11527
                     /gene="ompR"
                     /locus_tag="RH057_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HDV3128090.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            11524..12876
                     /gene="envZ"
                     /locus_tag="RH057_001708"
     CDS             11524..12876
                     /gene="envZ"
                     /locus_tag="RH057_001708"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HDV3128091.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(12952..14574)
                     /gene="pckA"
                     /locus_tag="RH057_001709"
     CDS             complement(12952..14574)
                     /gene="pckA"
                     /locus_tag="RH057_001709"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HDV3128092.1"
                     /translation="MRVNNGLTPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTKQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            14953..16677
                     /locus_tag="RH057_001710"
     CDS             14953..16677
                     /locus_tag="RH057_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HDV3128093.1"
                     /translation="MDNVELSPATRWGMIATGLLQGLVCYLLIAWLAGKNHSWIVYGV
                     PATVAFSSVLLFSVISFKQKRLWGWLALVFIATLGMSGWLKWQTDGMNPWRAEKALWD
                     FGCYLLLMAMLLLPWIQQSLRIRNDSSRYRYFYQSVWHNVLILLVIFLANGLTWLVLL
                     LWSELFKLVGITFFNTLFFATDWFIYLTLGLVTALAVILARTQSRLIDSIQKLFTLIA
                     TGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAIVRDPQKASL
                     PWTGPLRCLIKTALLAAPLYVFVAAWALWLRVAQYGWTVDRLQGVLAVLVLLVWSLGY
                     FVSIVWRKGQNPVVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSHMARYQSGKN
                     TSDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQHLQEQVSEKV
                     LAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDGRAERILFAF
                     DDERYIVYGFDPDKKEWQELTMSLLPRDITKEKLLTAAKDGKLGTKPKAWRDLVVDGE
                     RLDVNLNE"
     gene            complement(16740..17618)
                     /gene="hslO"
                     /locus_tag="RH057_001711"
     CDS             complement(16740..17618)
                     /gene="hslO"
                     /locus_tag="RH057_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417860.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HDV3128094.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMSLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNAQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVKFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADPQVH"
     gene            complement(17643..18044)
                     /gene="hslR"
                     /locus_tag="RH057_001712"
     CDS             complement(17643..18044)
                     /gene="hslR"
                     /locus_tag="RH057_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HDV3128095.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMALAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            complement(18055..18723)
                     /gene="yrfG"
                     /locus_tag="RH057_001713"
     CDS             complement(18055..18723)
                     /gene="yrfG"
                     /locus_tag="RH057_001713"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HDV3128096.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            complement(18788..20923)
                     /gene="igaA"
                     /locus_tag="RH057_001714"
     CDS             complement(18788..20923)
                     /gene="igaA"
                     /locus_tag="RH057_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HDV3128097.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTGASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMKIQAYYESCLNPQLITPSESLIE"
     gene            21243..21803
                     /gene="nudE"
                     /locus_tag="RH057_001715"
     CDS             21243..21803
                     /gene="nudE"
                     /locus_tag="RH057_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HDV3128098.1"
                     /translation="MSKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLVGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            complement(21968..24520)
                     /gene="mrcA"
                     /locus_tag="RH057_001716"
     CDS             complement(21968..24520)
                     /gene="mrcA"
                     /locus_tag="RH057_001716"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417855.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HDV3128099.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVATLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTLMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQQATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            24640..25419
                     /gene="hofM"
                     /locus_tag="RH057_001717"
     CDS             24640..25419
                     /gene="hofM"
                     /locus_tag="RH057_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HDV3128100.1"
                     /translation="MAFKIWQIGLHLQQQEAVAVAIVRGAKECFLQRWWRLPLENDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYRWGRKLAVGMTSAKELAAALSVDPES
                     VAICGEGGFDPWEAVSVRQPPLPPSGGDFAIALGLALGKAY"
     gene            25419..25958
                     /gene="hofN"
                     /locus_tag="RH057_001718"
     CDS             25419..25958
                     /gene="hofN"
                     /locus_tag="RH057_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HDV3128101.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            25942..26382
                     /gene="hofO"
                     /locus_tag="RH057_001719"
     CDS             25942..26382
                     /gene="hofO"
                     /locus_tag="RH057_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HDV3128102.1"
                     /translation="MNMFFDWWFATSPRLRQLCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLETPHEG"
     gene            26372..26776
                     /gene="hofP"
                     /locus_tag="RH057_001720"
     CDS             26372..26776
                     /gene="hofP"
                     /locus_tag="RH057_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
                     /protein_id="HDV3128103.1"
                     /translation="MRVKRWLLAGIALCLLTGMRDPFKPPEDLCRISELSQWRYQGMV
                     GRGEHIIGVIKDGQKKWRRVQQNDVLENGWTILQLTPDALTLGTGTNCEPPQWLWQRQ
                     GDTNEAMDSRTTVDADTRRTGGKAAKSDADGG"
     gene            26688..27926
                     /gene="hofQ"
                     /locus_tag="RH057_001721"
     CDS             26688..27926
                     /gene="hofQ"
                     /locus_tag="RH057_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HDV3128104.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNNNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QGSEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            28327..28848
                     /gene="aroK"
                     /locus_tag="RH057_001722"
     CDS             28327..28848
                     /gene="aroK"
                     /locus_tag="RH057_001722"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HDV3128105.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            28905..29993
                     /gene="aroB"
                     /locus_tag="RH057_001723"
     CDS             28905..29993
                     /gene="aroB"
                     /locus_tag="RH057_001723"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HDV3128106.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTNET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLKRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEIRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            30085..31371
                     /gene="damX"
                     /locus_tag="RH057_001724"
     CDS             30085..31371
                     /gene="damX"
                     /locus_tag="RH057_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HDV3128107.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPAATDGQQRVEVQGDLNNALTQPQNQQPLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPATTRPARQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPVAPVASTKAPAATSAPAPKETATTAPVQTASPAQTTATPAAGGKTA
                     GNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVSGV
                     YASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            31478..32314
                     /gene="dam"
                     /locus_tag="RH057_001725"
     CDS             31478..32314
                     /gene="dam"
                     /locus_tag="RH057_001725"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV3128108.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMARADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            32332..33009
                     /gene="rpe"
                     /locus_tag="RH057_001726"
     CDS             32332..33009
                     /gene="rpe"
                     /locus_tag="RH057_001726"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HDV3128109.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALATGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            33002..33760
                     /gene="gph"
                     /locus_tag="RH057_001727"
     CDS             33002..33760
                     /gene="gph"
                     /locus_tag="RH057_001727"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HDV3128110.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPIAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     SEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            33753..34757
                     /gene="trpS"
                     /locus_tag="RH057_001728"
     CDS             33753..34757
                     /gene="trpS"
                     /locus_tag="RH057_001728"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HDV3128111.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFDYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
BASE COUNT         8069 a         8936 c         9441 g         8324 t
ORIGIN      
        1 tatgccgtga cgcatcgaac ggcacgcctg acttcagcac tccatacgcc acctgtgcca
       61 gcttgcgcat catcgcgccg agaatcacct ttcctttctt gccattagcc gccagacggt
      121 cgcggaacgc ccgtccccac tcagtcttac tggtggctac cattgcgggc atatacaacg
      181 ccctgcgaag cgacacatgt ccggccttac tcatccggct cgcccctctc acactgctac
      241 ctgattcata acgccgtggt gtcagacccg caaaagcggc gaactgtctg gcatgggcga
      301 agcggtcctt cagaccgata taagccagca ataccgcaga tgttttctct ccgatacccg
      361 ggatgctttc cagcagtttc ctgcggtgtt tcatatccgg atcatcgtct gtcaggtctt
      421 ttatctgctt ctcaagacgc ttcagctctg cttcaagcca cagaaggtga gcatcaatgc
      481 tcggtctctg gacttcccgc gccgtttcag tgcgattcag ttcctgcgtg tgcatatctg
      541 tcagcgcctg gtggcggact accagggcac gcaacgcgcg ttcaagcggg tgaggcgctt
      601 cccaggctgc agggcgcttc tgacgacaga actctgccag catgcgcgca tccacggtat
      661 cagtcttgtt acgcagtcct tcactctgag cgaaagcttt acccagcgca ggattaatga
      721 ctgacactat gtagccagca tcgtaaaggc actcagcgac aggttccata taggtgccgg
      781 tcgcttcgat gcagatatgc gcatggtcaa tcttgtgacc tttcagccag ctcaccagct
      841 catcgtgccc tttagtggtg ttagcgaatt ttttggtgcg atgacgacca tcaggacgca
      901 acacatcgac atccagtttc tctttagcgg tgtcgatacc gatataatga agttcatgtt
      961 ccatagtgaa accaaccttg caaatacgga ttaccgggaa aaccggtcca tgatactgtc
     1021 cggtttatca ctttgggaga aaggcagtcc gctgcataaa tctacgcaac aggttagaac
     1081 ccaaggcccg atacggcatg cggactgcag gcagagaaaa ctggccgatt ttctctgcac
     1141 tggagggata atacaaggcc cgaaatgacg ctttagcgtc gcatcgggca atctacaaaa
     1201 gaggggataa cttagtagta ggagtgttcg ccgcgctggt gttcggtgag atcgcgcaca
     1261 cctttcagct ccgggaattc gttcagcagc tgcttctcga tcccttcttt cagcgtcaca
     1321 tcgaccatgg aacaaccgtt acagccgccg ccaaattgca gaatggcgta accgtcttcg
     1381 gtgatttcca tcagcgaaac gcgaccaccg tgaccagcaa gctgtgggtt gatctgcgac
     1441 tgcagcatat actccacgcg ctccatcagc ggtgcatcgt ctgccacttt acgcattttg
     1501 gcgttcgggg ctttcagcgt taactgagaa cccaactggt cggtaacaaa gtcgatctct
     1561 gcatcttcca ggtatggtgc gcttaactca tcaacatacg cggtcagcag gtcaaatttc
     1621 agggctgtgt cggtggcttc cacagcgtcc ggcggacaat aagaaacgcc acattcagcg
     1681 ttaggcgtgc cagggttaat cacaaatacg cggatttgtg tcccttcttc ctgatttgcc
     1741 agcagtttgg caaagtgcgc ttgtgcagca tcggaaatac ggatcatagt aatggcctaa
     1801 tagttgacta ttttagttgg ttataatacg cccatcatcg aggctctaca aggttcgaca
     1861 aaggcaccag acctggacag ccgccgcacc attgcgtaaa agcaactgcg caatctctgc
     1921 gacggtactt ccggtggtaa cgacatcatc cacaatcacc atatggcgac cttgcacggg
     1981 caattcaaga cgaaaggcat ttttcaggtt gcgcttgcgc agccgggcac tgagaaaatg
     2041 ctgggtcgca gtggcccgtg tacgtgtgac ggcttcgcta tcccattggc agtgcaacca
     2101 gcgtgataac ggctgacaca gcaaatcgct ctgattaaat ccccgacgcc agtgacgccg
     2161 ctgccataac ggaacgctga cgatgcgatc cggcaattgc aacccggtgg tgcgacgagc
     2221 gtgtaagact tccaatagta acagacgtga cagggcgctg gcgatttcac tgcgccggga
     2281 aaatttaagc tggtggataa gcggacttaa cggcggcgca tagtcggcaa ccgtgaccag
     2341 tctttgccag ggcggcggtt tttgcaggca gcgaccgcag ggaagatggg agtgtgtggc
     2401 gggtaatcca cattgtgggc ataacgtttt atctgtgcgg gtggcgcgtg aacagaccga
     2461 acaaatcccc caatgaccta acgccagtgg cattcggcat agccagcata atcccggtac
     2521 tgttagcata tgttcatcct tgtaagtcaa aagagaacaa tagcggatga ataacatctg
     2581 gtggcagacc aaaggtcagg ggaatgttca tcttgtgctg ctgcacggat ggggactgaa
     2641 tgccgaagtg tggcgttgca ttgacgagga acttagctcg cattttacgc tgcaccttgt
     2701 tgacctgccc ggcttcgggc gtagccgggg atttggtgcg ctgtcacttg ctgatatggc
     2761 cgaagccgtg ctgcaacagg cacctgataa agccatttgg ttaggctgga gtctgggcgg
     2821 gctggtggca agccagattg cgttaaccca tcccgagcgt gttcaggcgc tggtcaccgt
     2881 ggcgtcgtca ccttgtttta gtgctcgtga cgagtggccg gggataaaac cggacgtgct
     2941 ggcgggattt cagcagcaac tcagtgatga ttttcagcgt acagtggagc ggttcctggc
     3001 gttacaaacc atggggactg aaacggcgcg ccaggatgcg cgggcgttga agaaaaccgt
     3061 tctggcgtta ccgatgccgg aggttgacgt gcttaatggc gggctggaaa tcctgaaaac
     3121 ggtcgatctc cgtcagccgc tgcaaaacgt gtccatgccg tttttgcgat tgtatggcta
     3181 tctcgacggt ctggtgccgc gcaaagtggt gccgatgctg gataaacttt ggcctcacag
     3241 cgaatcatat atcttcgcca aagcggccca tgcgccgttt atttcgcatc cggtcgagtt
     3301 ttgtcacctg ctggtggcgt tgaagcagag ggtgtaggca acttttgaaa tggcgagaca
     3361 gaaatttact ggctcgccgc agccaactct tcttctgaca ccccagtaaa gcgcatgatg
     3421 tctgtaagag ggactccgga ttcaagcatt attttggcta tatgcagggc tttggattgt
     3481 tccccctcct gccgcaatct ttccgcaatt gtcattaaac tctccttgtg tttcggtgaa
     3541 cgttcggcaa cgccgtcgat aaaatcgtta aaacgtaccg cgtcgccggt ttgcagtatg
     3601 taattaaaca gccctttgat ttgtctgtca ttagcgtatc cactacttaa taagcaggcc
     3661 atttgctcta ccagccccat caggtcgcgt tgacgaatat gtttttgaat taactccagc
     3721 agcgccatac gtcggtgctg catgatttca tcatcaggca tgacggtgac atcaatcagc
     3781 ggaaatgcgg aggcataaag ctgccttgcc agtttgggat cggcgaaaca atccagccaa
     3841 cacagcgaat agggataggg gctttcaata ccgtggtaaa acaacaatag caccaccatc
     3901 ggcaacgttt tgtatccagc atcaaggtga ttttgcattg cggcaatagc ttaacgcatc
     3961 atgcgaaatg cgatcagttt gtttgaggtg ctttgatgtt caatcagaca atagatgtat
     4021 cctggtcctt gttccgtttt caccgaccac agcacatcgg aatagctttc acgcagatcg
     4081 tcatcaataa agctgctcga ctccagcttg agggttttca tatcacagag cgcgtgaatg
     4141 ggggccggta aatggaacgc aagaaaatcg cgagccgtgt ccggttggcg taaaaagagt
     4201 ttgaacagcg catcatgtgg ggtggaactc tgctttttac tcatggcgtt ccttcacctg
     4261 ttgtttgaat gccgcgactc tacgcgccac tccacatctg tgcattagcg agttttcatc
     4321 cctgcgagat gctttcaaag gaacttacgc cgtgctgcaa gagttgcatt tttatggaaa
     4381 gccgcacgca aaaaagcccg gatgcagacg cataccgggc tttccaaaac aggcgatgga
     4441 gtaaggttaa cgcagcgccc accactcgcg cagacaggct tttccttccg ggcagctttt
     4501 acaactgcca gagaggcagc cgtcaggttc ttcctgaatc cgcacggctt tgcccatact
     4561 ttccagttgt tgcagcatgg cgttaatcat tggctgtgga gtgttcaatg tctggcttat
     4621 ctgggccgct tccatacggc cccgtaacgc cagcaaatcg cgcacctgaa taagtgaagc
     4681 cattaatggc aatcaccggt ggtgctggct gcgcagcaac tgcttaccga cttgcgggtt
     4741 gccagcagtt cgatatccac ccggctacgc gcgcggcgca gcagaccgat aaccacgatg
     4801 ttaaacagga taaccgccag aatgcacacc aggctgtaag ttgggtgctg actgtagctg
     4861 gcgacctgat agaacaatgt tgccagcgag taagcgatat tcagccccca caggatggag
     4921 aagcccatcc agccacggct tgattcacgg gcgatggctc ccatcaccga gatacatggt
     4981 acatacagca ggacgaaaat caggtagctg taagctgctg ctgcgctacc gaatttctga
     5041 tccatcacgc ccatcgcccc ggtacccatt tcgccgtcgc ctttgctggc ttcaatgggg
     5101 ttcatcagta cgctaaggct gaaggtgtct ttcaggctct gccaggtttc atctatcgca
     5161 ctgaacagct cttcaccgag gttaaactct gccggattga actcttcgtc ctgaatattt
     5221 tctgcggtgt agagggtgtt gagcgtaccc actaccactt ctttcgccat ggcacctgta
     5281 aacaggccaa ccgttgcctg ccagttatct tcatgcacgc caattggctt gaagaccggg
     5341 gtgatcaccc ggctgacgga cgccagcgcc gagtcgttga tgttatcgac gattttcccg
     5401 ctcagcgaga agctgttgaa agcgctcagg aaaatgctga cgatgatgat cactttacca
     5461 gcacgcagaa cgaagccttt cagacgctgc caggtctgga taatcaggct tttaacgtgt
     5521 ggtacatgat agaccggcaa ctccatgaca aacggcgtcg cttcaccgcg catgatggtg
     5581 tacttgagca tcaggccagt cagcaccgcc atcacaatac ccagcatata cagcgagaag
     5641 accgccagcg caccgttctg cccgaagaag gcagccgcga atactgcgaa gatagccaga
     5701 cgcgcgccgc aggacataaa cggtgccatc atgatggtca tcagacgttc acgcggtgca
     5761 tcaagcgtac gtgcacccat taccgacggt acgttacaac cgaaaccgac gatcagcggc
     5821 acaaaggatt tccccggcaa gcccagcgcc tgcatcagac ggtccatcac aaacgccgca
     5881 cgcgccatat acccggagtc ctcaaggaag gagaggaaca ggtacatcat gccaatctgc
     5941 ggcaccagtg gcagcacggt gttaataccg ccacccaggc cctgggcgag gaagatagtc
     6001 agccagtccg ggaagtggag cgtgtagcca atccattgaa taccatgcac aaatagcgcc
     6061 acggagccga cgtcaaacag cggctgtaac gccccgccga tgttgatagc cagcaggaac
     6121 atcaggtaca tcacaaagag gaaaatcggc agaccgagga aacggttgag cacgatttta
     6181 tctaccgcag tggtgaaacg gctgggttct gccgtcaggg tgttgcttac cacatcacag
     6241 atggcagcaa tgcactggta acgcgcatcg gcaatgtgca gcgccggatc gtccatctca
     6301 ttacgcagac gggcgagggc ggcatccaga tgctgcgacg cttcaccggc gtaggcgcgg
     6361 ctgtagatat cgccttccag catttgcagg cccagccagc gacgttgttt cagcgggatg
     6421 tcggaaggca tcacttttgc cagtgaatct gcttcgttga gtagcggctg tgcgtaatgc
     6481 accagttcca cattctcgtt agctttatag cgatcaatcg ccagcttgag cgcttcaata
     6541 ccgcgaccac gggttgaaac cagcgggatc actgggcagc caagacgcgc cgacagagca
     6601 tcaatttcaa tacgaatatt ttgcttctcg gcaatgtcga gcatgttcag tgccacgatg
     6661 cagggaatgc cgagttccag cagttgtagc gtcaggtaca ggttacgctc aaggttagac
     6721 gcatccacca cgttaatcag caggtcggcg tcgccactca aaatgtagtg acaggcgatt
     6781 tgctcatcga gcgaggtctg cgatgagatg gtggtcagag aataggtgcc gggcaggtcc
     6841 accagcgtga cctgatgatc ggtggtggag aattgccctt ctttacgttc gacggtaacg
     6901 ccagcccagt tacctacacg ctgacgtgcg ccagtgagct ggttaaataa ggttgtcttg
     6961 ccagaatttg gattaccaat taagcctatg gttaattttt tcattgttgt tatcaccgta
     7021 ttaacaggaa accgcttcca cttctaataa ggccagatct tttttgcgta ataccaggct
     7081 cacacgacgg gtttcgatat gaatggggtc gccgagtgga gcgacgcgca ccacattaaa
     7141 agaggagcca ggtaacatgc caagagaaag cagtttttgg cgatatgccg ggctgatttc
     7201 acgggaaaag ccagtgattt tccacgcagt atctggagtg tattgcatag gtgcctactt
     7261 gtttctcatt aactggataa ctacctcatg gggcgtttga cagcgacatg agaccagatg
     7321 atttgccaac aaaggagaga ttaaataatc aacaacacaa tgataatgag aatggtttct
     7381 atcagcaata cttaaaatgt gtgcgaatgt tggtttgaat aataatctga ctcaatgttg
     7441 atgtggctca aggttatatc gcagcgaaaa tataattcga atagcgaatt accaggactg
     7501 gctaatgttt aattaaggtt tcagttgtta atgaagtgat aaatgttgcc ggaatatatt
     7561 gtgagtttgg ctgtggaata aaaatgccgg agatattctc cggcactttt atgtcagaca
     7621 aggcgttcgt tgccagatgc ggcgtggaca ccttatcgcg caggtttagg ggtcaatcag
     7681 ggcgtttaac gttttttgcc cattgctgcc gccagcgcgt ccatcatcgc gctgttaccg
     7741 gcaggctgcg cttcacgtcc gcgcggtttg gctgccgggc ggttgttttg cgggcgttca
     7801 ttaccgccgc cgcgacgggc gttggtttcg ccaggctgct cgtccagacg catggtcagg
     7861 gcgatacgtt tacgctgaag atccacttcc agcactttca ccttcacaat gtcgcccgct
     7921 ttcaccacgg tatgcggatc ttctacaaac ttgttcgaca atgaagagat gtgaaccagg
     7981 ccgtcctgat gcacgccgat atcgacaaac gcgccaaagt tggtgacgtt ggtcaccgca
     8041 ccttcgagga tcatacccgg ttgcaggtcg ttcattgtct cgacgccatc ggcaaactga
     8101 gcggttttaa attccggacg cggatcgcga cccggttttt ccagctcttt gatgatgtca
     8161 gttactgtcg gcacaccgaa tttttcatca gtaaagtcag acgctttcag gttacgcagt
     8221 tcgctgctgt tacccatcag atctttcagt gcctgctgtg ttgctgccag aatgcgttcc
     8281 accaccggat aggcttccgg gtggacggta gacgcgtcca gcgggttatc accatggtta
     8341 atgcgcaaga agcccgcgca ctgctcgaag gctttcggcc ccagacggct cactttcagc
     8401 agttgctgac ggttctggaa ctgaccgttc tcatcgcgcc aggccacgat gttttgcgcc
     8461 atcatgcgcg tcaggcccgc cacgcgggtt aacagcggaa cagaagcagt gttgagatcg
     8521 acgccaacgg cgtttacgca gtcttctact actgcgtcca gtttgcgggc cagttgcgtc
     8581 tggctgacgt catgctgata ctgacctacg ccgatagatt tcggatcgat tttcaccagc
     8641 tccgccagcg gatcctgcaa acggcgggcg atagacaccg cgccacgcag cgaaacgtcg
     8701 agatccggaa actcctgcgc tgccagctcg gaagccgagt aaaccgacgc gccagcttcg
     8761 ctgacgatca ctttctgtgc ggtcactttc gggaactgct tctgcacgtc gagatagaaa
     8821 cgttcggttt cgcgggaagc tgtaccgttg ccgatcgcca ccagttcaac gttatgcttt
     8881 tcacacagcg cagcaacagt catcgctgct tttgcggctt gtccggtgtg cgggtaaatg
     8941 gtatcggtcg ctaccagttt gccagtggca tcgaccaccg ccacttttac cccagtacgc
     9001 agacccggat cgaggcccat cgttgcacgc agtccggcag gggccgccat cagcagatcg
     9061 tgcaggttac gggcaaagac gttgattgct tcatcttccg cgcgttcgcg cacggtaccc
     9121 atcagttcgg tttccagatg catcagcacc ttgatgcgcc aggtccagct cactacgcct
     9181 ttgcgccagc tatccgccgg ggcattgttc aggcgcaggc caaggtgatc catgatgatt
     9241 tgctcgcaat agctctcttt gggcggctca tcgaactgcg gatcggcatt cagcgaaagc
     9301 tggagtacgc cttcgttacg cccacggaac atcgccagcg cgcggtgaga aggcaccgtg
     9361 gacaacggtt cgtgatgatc gaaatagtcg cggaattttg ccccttcctc ttctttaccg
     9421 ctcaccacgg ttgagaccag atgcgcgttc ttccacagat aatcacgcac tttcgccagc
     9481 agcgcggcat cttcggcaaa acgttccatc aggatatagc gcgcgccgtc cagcgcggct
     9541 ttggtatctg ccacgccttt atcggcatca acatattgtg cagcggcgac ttctggcgtg
     9601 tgtgacggat cgctccacag caggtcagcc aacggctcaa gccctgcttc aatggcgatt
     9661 tgcccgcggg tgcggcgttt aggtttgtag ggcaggtaga ggtcttcgag ttcggttttg
     9721 cttagggtgg cgttgatggc cttcgccaga tcatcggtga gtttgccttg ctcggaaatg
     9781 gatttgagga tcgcctgacg tctctcttcc agctcgcgca gatagctcag acgcgtttcc
     9841 agattacgca gctgcgtgtc atccagaccg ccggtgattt ccttacgata acgtgcgata
     9901 aacggcacgg tattcccttc gtcaagcagg cgaacggcag cgtcaacctg ttccgggcgc
     9961 gcctgaattt cacccgcaat aatgcggcag aacgaatcat tcatcatggt ttgagttcat
    10021 cttttcggat caaaaatcag gggatagtta tacggactgg ctggcaaaaa tgccagccat
    10081 cggcaggagg ttaagactct tccttacggt ttcacgtact cgatagcatt aacataccag
    10141 ctcgcttccc cggcaggggt attcaccacc gccagatcgc cgacttcttt tttcagcaat
    10201 gcgcgggcca tcggggaatc gatagagatg taatctttac ggccaaaaat ttcatcgtag
    10261 ccgacaatac ggaaacggtg agtcacgcca tcgtcgtttt caatctccac ccacgcgcca
    10321 aaaaagactt tgccttcctg ctgaggggag taatcgacga ttttgagatt ttccaggcat
    10381 ttagtgagat agcgcacgcg acggtcgatt tcacgcagac gctttttatt atactgatag
    10441 tcagcatttt cgctgcggtc gcccagactt gcggcccagg tcaccttttt tgtgacctcc
    10501 gggcgttctt cacgccagag ataattaagc tcttgtttga gtttttcata cccttcccgg
    10561 gtaaccaggg gcgttttcat ctcgttgatt ccctttgtct gtttgataat gcgcacattg
    10621 ggtataacgt gatcatatca acagaatcaa taatgtttcg ccgaataaat tgtatactta
    10681 agctgctgtt taatatgctt tgtaacaatt taggctgaaa ttcataccag atttagctgg
    10741 tgacgaacgt gagctttttt aagaatacac gcttacaaat tgttgcgaac ctttgggagt
    10801 acaaacaatg caagagaact acaagattct ggtggtcgat gacgacatgc gcctgcgcgc
    10861 gctgctggag cgttatctca ccgaacaagg cttccaggtt cgaagcgtcg ctaatgcaga
    10921 acagatggat cgcctgctga ctcgtgaatc tttccatctt atggtactgg atttgatgtt
    10981 acctggtgaa gatggcttgt cgatttgccg acgtcttcgt agccagagca acccgatgcc
    11041 gatcattatg gtgacggcga aaggggaaga agtggaccgt atcgttggcc tggagattgg
    11101 cgctgacgat tacattccaa aaccgtttaa cccgcgtgaa ctgctggccc gtatccgtgc
    11161 ggtgctgcgt cgacaggcga acgaactgcc aggcgcaccg tcacaggaag aggcggtaat
    11221 tgctttcggt aagttcaaac ttaacctcgg tacgcgcgaa atgttccgcg aagacgagcc
    11281 gatgccgctc accagcggtg agtttgcggt actgaaggca ctggtcagcc atccgcgtga
    11341 gccgctctcc cgcgataagc tgatgaacct tgcccgtggt cgtgaatatt ccgcaatgga
    11401 acgctccatc gacgtgcaga tttcgcgtct gcgccgcatg gtggaagaag atccagcgca
    11461 tccgcgttac attcagaccg tctggggtct gggctacgtc tttgtaccgg acggctctaa
    11521 agcatgaggc gattgcgctt ctcgccacga agttcatttg cccgtacgtt attgctcatc
    11581 gtcaccttgc tgttcgccag cctggtgacg acttatctgg tggtgctgaa cttcgcgatt
    11641 ttgccgagcc tccagcagtt taataaagtc ctcgcgtacg aagtgcgtat gttgatgacc
    11701 gacaaactgc aactggagga cggcacgcag ttggttgtgc ctcccgcttt ccgtcgggag
    11761 atctaccgtg agctggggat ctctctctac tccaacgaag ctgccgaaga ggcaggtctg
    11821 cgttgggcac aacactatga attcttaagc catcagatgg cacagcaact gggcggcccg
    11881 acggaagtgc gcgttgaggt caacaaaagt tcgcctgtcg tctggctgaa aacctggctg
    11941 tcgcccaata tctgggtacg ggtgccgctg accgaaattc atcagggcga tttttccccg
    12001 ctgttccgct atacgttggc gattatgcta ttggcgatag gcggggcgtg gctgtttatt
    12061 cgtatccaga accgaccgtt ggtcgatctc gaacatgctg ctttgcaggt cggtaaaggg
    12121 attattccgc cgccgctgcg tgaatatggc gcgtccgagg tacgttcggt tacccgcgcc
    12181 tttaaccata tggcggctgg tgttaagcaa ctggcggatg accgcacgct gctgatggcg
    12241 ggggtaagcc atgatctgcg cacgccgctg acgcgtattc gcctggcgac cgagatgatg
    12301 agcgagcagg atggctacct ggcagaatcg atcaataaag atatcgagga gtgcaatgcc
    12361 atcattgagc agtttatcga ctacctgcgc accggacagg agatgccgat ggaaatggcg
    12421 gatctcaacg cagtactcgg tgaggttatt gctgccgaaa gtggctatga gcgggaaatt
    12481 gaaaccgcgc tttaccccgg cagcattgaa gtgaaaatgc acccgctgtc gatcaaacgc
    12541 gcggtggcaa atatggtggt caacgccgcc cgttacggca atggctggat caaagtcagc
    12601 agcgggacgg agccgaatcg cgcctggttc caggtggaag atgacggtcc gggaattgcg
    12661 ccggaacaac gtaagcacct gttccagccg tttgttcgcg gcgacagtgc gcgcaccatt
    12721 agcggcacgg gattaggact ggcgattgtg cagcgtatcg tggataacca taacgggatg
    12781 ctggagcttg gcaccagcga gcggggcggg ctgtccattc gcgcctggct gccagtgccg
    12841 gtaacgcggg cgcagggcac gacaaaagaa gggtaaaaaa acgggaggcg aaggtgcctc
    12901 ccgttttgct ttctctaaga tactggatag atattctcca gcttcaaatc attacagctt
    12961 cggaccagcc gctaccagcg cggcacccgc tggggtgtcg gtgtatttat cgaagttgtc
    13021 gataaacagt ttcgccaggg tttcggcttt ttcctgccac tgttccgggg aagcataggt
    13081 gttacgcgga tcgagaatct tcgtgtctac gcccggcagt tcggttggga tcgccaggtt
    13141 aaacatcggc agagtgaaag tttctgcgtt atccagcgaa ccgttgagga tggcgtcgat
    13201 aatggcgcgg gtatctttaa tcgagatacg tttgccagtg ccgttccagc cagtgttaac
    13261 cagataagcc tgcgcgcccg ccgcctgcat acgtttcacc agcacttctg cgtactgagt
    13321 cgggtgcagc gacaggaatg ccgcgccgaa gcaagcggag aaggttggcg tcggttcggt
    13381 gatgccacgc tcagtaccgg ccagtttggc ggtgaagcca gagaggaagt ggtactgggt
    13441 ttgatcggca gtcagacgag aaaccggtgg cagcacacca aaggcgtctg cggtcaggaa
    13501 gataacttta gttgcgtggc ccgctttgga aaccggctta acgatgttat cgatgtgata
    13561 gatcggataa gaaacgcggg tgttctcggt ttttgaacca tcatcaaagt cgatagtgcc
    13621 atcttcacgc acggtgacgt tttccagcag cgcatcgcga cggatagcgt tgtagatttc
    13681 aggttccgct tctttcgaca gcttgatagt ttttgcgtag cagccgcctt cgaagttaaa
    13741 cacgccgtca tcgtcccagc cgtgttcgtc atcgccaatc aggcgacgtt tcgggtcggt
    13801 ggaaagggtg gttttaccgg tgccggaaag gccgaagaac accgcaacat cgcctttctc
    13861 gccaacgttg gcggagcagt gcatagaagc gatacctttc agcggcagca ggtagttcat
    13921 catcgagaac atccctttct tcatttcgcc gccgtaccag gtgccgccaa tcagctgcat
    13981 gcgctcggtc aggttaaacg ccacgaagtt ttcggagttc agaccctgtt ctttccactg
    14041 cgggttagtg cacttcgcgc cgttcataac gataaagtct ggtttgaaac ctgccagctc
    14101 ttcatcgctc gggcgaataa acatgttttt gacaaaatgc gcctgccagg ccacttcggt
    14161 gatgaaacgg acggaaagac gagtatccgg gttcgcacca cagaaagcgt cgacaacgaa
    14221 cagacgtttg ccggaaagct gtttggtcac caggcctttc agatgttgcc aggtttccgg
    14281 agagagaggt ttgttatcgt tcttaccttt gcctttgtct gcccaccaga aagtatcgcg
    14341 agtggtatcg tcacggacga tatacttatc ttttggtgaa cgaccggtga agatcccggt
    14401 atcgacggca acggcaccca gattagttaa caccccgcgc tcataacctg tcaggctcgg
    14461 atcgagctct tcctgataca gcaggtcgta gcttgggttg taaacgatat catgtacgtc
    14521 actgatacca taagcctcga gttcttgcgg ggtcaaacca ttgttaacgc gcatttcact
    14581 gctccttagc caatatgtat tgcctgaata gtaaagtctt tttgggggtg ttaaccgcga
    14641 caaggctcat agatttacgt atctggagaa attcatattt gacaaaaaac tccgtgattc
    14701 ctgtcacgaa acggttgcta ttatcgcaga aatagcattt taggtgtgga aaatgttctc
    14761 aaaaggttaa atcttggttt atatggcggg aatattgtga gtggaatcgc attcatgaat
    14821 gaaattgatg caaatttaat cggtaaagta tcgattcacc acgctaactt tttttgatta
    14881 ttttctgaaa aaagcaaaat actactgccc ggatgcgata attaacccac cttttgattc
    14941 tggataacca acatggataa cgttgaactt tcaccggcaa cacgctgggg gatgattgct
    15001 accggattac ttcagggtct ggtttgctac ctgctgatcg cctggcttgc cggaaaaaat
    15061 cacagttgga ttgtttatgg cgtgcctgcg accgtcgcgt tttcatccgt tttactcttc
    15121 tccgtgatct cttttaaaca gaagcgcctt tggggatggc tggcgctagt gtttattgcc
    15181 acgctgggga tgagcggctg gctgaagtgg caaactgatg gcatgaaccc ctggagagct
    15241 gaaaaggcgc tttgggattt tggttgctat ctgctgctga tggcaatgtt gttgctaccg
    15301 tggatacaac aaagccttcg catccgcaat gacagtagcc gctatcgcta tttttaccag
    15361 tcagtatggc ataacgtact tatattactg gtgatttttc tcgccaatgg cttgacgtgg
    15421 ctagtgcttt tactgtggag tgagttgttt aaacttgttg gcatcacgtt tttcaatacg
    15481 ctcttttttg caaccgactg gtttatttat ctcacgttag gtctggttac cgcgctggcg
    15541 gtgatcctcg cgcgaacaca gtcacgttta atcgactcta ttcaaaagtt gttcacgtta
    15601 atcgccacgg ggttgctgcc gttagtatca ttgctaaccc tgatgtttat catcaccctg
    15661 ccgtttacgg gcctgagcgc gatttctcgc cacatctccg ccgccgggtt gctgttgacg
    15721 ctggcctttt tgcaattgat cttaatggct attgtccgcg atccgcaaaa agcgtcactt
    15781 ccctggacag ggccgttgcg ttgcctgatt aaaaccgctt tgctggctgc tccgctgtat
    15841 gtgttcgtcg ccgcctgggc gttatggctg cgggtcgctc agtacggctg gactgtcgac
    15901 cgcttgcagg gcgtgctggc ggtgctggtg ttactggtgt ggtcgctggg gtattttgtc
    15961 agcatcgtct ggcgtaaagg gcaaaatccc gttgttcttc agggcaaagt gaaccttgcg
    16021 gtttcgttat tggtgttggt gatactggtg cttcttaatt cgccggtgct ggacagtatg
    16081 cgcattagcg tgaacagtca tatggcgcgt tatcagagcg gcaaaaacac gtcagaccag
    16141 gtaactatct acatgctcga gcagagcggt cgctatggac gtgctgcgct tgagtcgctg
    16201 aaaagcgatg ccgggtttat gaaagacccg aaacgcgcgc gggatctgct gatggcgtta
    16261 gatggagagc aacatcttca ggaacaggta tcggaaaaag tattagccga aaatgtgtta
    16321 attgcccctg gttctgttaa accggatgcg acattctggt cggccttaat ccaggatcgc
    16381 tataacgtga tgacctgcat tgaaaaagac gcctgcgtcc tggtcgagca agatctgaat
    16441 agtgatggca gggcagagcg gatcctcttt gcctttgatg atgaaagata cattgtctat
    16501 ggctttgatc ctgacaagaa agaatggcaa gagcttacga tgagtttact tccgcgtgat
    16561 ataacgaaag aaaaattact cacagctgcg aaggatggaa aactgggaac gaagcctaaa
    16621 gcgtggcgcg atcttgtagt ggatggtgaa aggctggatg tgaatctgaa tgagtgacaa
    16681 agcgcatcag gtgtattagt cgtaaaatcc ggcagagtcc tctctgccgg acatactcat
    16741 taatgaactt gcggatctgc cggagacgcg ttgttgcgga tttcagcaat atccatcgca
    16801 ttgaacagat agtggttacc gcagtaatca caatgcatgt caatttcgcc atcttccgcc
    16861 aggatgctat cgacttcttc atcaggcagc gttttcagcg cgtcggcgca acgttcacgc
    16921 gagcaggtgc atttgaactt tacatcctgc ggatcgtaaa ccgtcacctc ttcttcgtga
    16981 tacaaacgcc acaacacttc gtttgccggt aaggtcagca gttcttcggt tttgatggtt
    17041 tcggttagcg tcgccaggtg gtcaaagtcg tcctgctggg cattttgcgc aggcattacc
    17101 tgcaacaaca taccgcctgc agccggttta ccgtctacgt cgccggtgcg aataaacagg
    17161 cgcgtcggca gctgttcaga acgcataaag taatcttcca ggcaggccgc cagggtatca
    17221 ccttccagac caaccacgcc ctgatagcgt tcgccttcgc tcggggtaat ggtgatcacc
    17281 acgtaaccat tgccgaccag cgttttcagg tcggcatttt ccggaatttc gccctgtacg
    17341 cgagccacac cgcgcatctg ctggttgttg tttccgttga tgaccgccag actcatcggg
    17401 ccgtcgcctt gcagttgtac ggtgatatca ccatcaaact tcaacgtagc ggttaacagg
    17461 ctggtcgcaa ccagcagttc cgccagcacg tttttaacgg gctgcggata atcgtgattc
    17521 tcaaggatct gttgcagggt ttccgaaacg gttaccagtt cgccgcgcac ggcaaagttt
    17581 tcaaacagat agcgatgtaa ttggtcatgt tgcggcataa tcatctctct tgcaggtgac
    17641 agttattcac tgtcgccgtg tttaaatcgt aacaggtcgc ggcgctcttt tttgtccggg
    17701 cgtcggtccg ggtgcggcat ggttaaggca ttaagtttac gtgccagcgc cattttttcg
    17761 cgtttctcta cactttccgc agtctcttca tacagcaagg ctgcctcgct ggcggggcga
    17821 cgctgttcag taatcgcctt tacaatcacc gtgcgttcgt catttccctg gcgcagagtg
    17881 agcgtggcat tcagctcgac gattttgctc ggcttgctgc gctgcccgtt gtaatgcacc
    17941 ttaccgcctt caatcatttc acgggccagc gcgcgggttt tataaaaacg ggcagcccat
    18001 agccatttat ccagtcgaac ctcaacagca ggtttttctt tcatggcgtc tccttcacat
    18061 tagcgagggg atcaggcggc ggtagtcatt cagtgacgga tggcgttgat actgtttctc
    18121 ggcaatcccg gagtcaggat tagtcacgcc gaggcagtaa cgaataccaa attgcgcggc
    18181 agcatcgaga atcgcttcgc tgtcatcaat aaacagcgtt ctttcagctt tcagacccgt
    18241 agcttcggcc accgcatgcc ataaccgctg atcctctttc ggataaccaa atgtgtgggt
    18301 ggaaagtaat aaatcaaggt gtgcgtccag accggtatgc tcaagtttta ccgccaggtt
    18361 gtgcggatgc gcattggtga gcaaaattcg ctgcttaccg ctggctttca gtgcctcaag
    18421 aaacggaatg gtatcttcac gcagtacggc acgcggtccc atctcggtgg tcatcgcaca
    18481 gatatccaga cccagttgct cactccagta atcaagacag taccagttta gcgtatgctg
    18541 tacgtcgtga tattgctggc gcatatattc catcgcttcc tgtggcgtaa ccccgttttt
    18601 cgcgccccat gtttcaggca ccagcttttg ccagaaatag ttatcgaagg cgaggtcgag
    18661 caacgtgccg tccatatcca gcagaacggt atctacgtcc tgccaggcaa tgttgatatg
    18721 catgagggaa atctccagag tgaagcaatt tgcgcgacag ggtagcataa cctgccgcgc
    18781 aaacgtgtta ttcgataagg ctttctgaag gggtgatcag ttgcgggttc aggcagcttt
    18841 cataataggc ctgaatcttc atcatgcgag tgcgatgacg ctggtaacgc cgccaggcct
    18901 gtacgccatt ataaatggcg ctacccagca tcgtcagtag cagcagcgtg gtgctgagat
    18961 agcgccacag gccggaacga tccgggatcg gatgaaggcc tatatgctgc gtaccattgg
    19021 cgtcagtgaa gatttttgtg acgataccct cggcgttaaa cggcgtatgc atcagcattt
    19081 gtgccagttt ctggaaagcg ttccactgtt cttgcggcgg atagtcgtaa agtgatgccg
    19141 aaggccaggg ctgatcaaca aaatcgctgc cttcgtcgct gacaatcagg aatccgccgg
    19201 gggccggact gtttagtgat tgtgccgctc gcgccgtttc atgcgtgata aacggcgcgg
    19261 tggaggttgc caccaggtta tccagcgatt ccgcactcac cgggcgtaat aacacattca
    19321 cgccatcgag cttcccggcg ttggcgcgtt ttaccagcgc gtcccagtct ttactgttgc
    19381 cgagattgac cagcgcattt ttcagtcgca cacagtcatc tttggcagaa cacagatccg
    19441 ctgtcttcag tacaatgtcg ccaaaatcat caagcaatac catgccggat ttttgaattg
    19501 ctgaacgtaa tgaggcactg acgcgagatt catcttccgg tttagggtgc agctggcgat
    19561 taactgcttc agtcaatgcc gtcgctttgt tgaccagttc agattctggt aatggcaatg
    19621 agcgggcgtc attccagatg atctgcgagc agtcaaacgg taaaaaaggt gaattggttt
    19681 tcgcgctcca ggttccggaa gtgcgaatat tacacattcc cgtaccgcta atacgcaatg
    19741 tatcgcctac ccgcacgcca gcgtcagcca gttgttttac gctggtggct tcaatggtct
    19801 gcgcgccttt catccatgag agagtgaatt tcagcggcat atccagcggg atccagaata
    19861 acagcataaa cagcaccagc agcgagcccg cagcgataat cgtactgcgc agccagtgct
    19921 gtaacggaaa gttttttact tcatcatgca gcgaaagata tcgcccctga cgcactacgt
    19981 ggcggtcgag atagatatcg atatcggttt gttgaccgag atcctgagca atgtatggct
    20041 gccagtgtgc gggatagacc aggtcgataa taccgagtga aatattgttg atctgttcct
    20101 gatcgttttc gccaaacaat ccccaacgac ggggtgtacc gcgcagacaa tgaatttccc
    20161 tcagggagga ttttgccggt ggcgcgaata gcccccacag accagcgccc agcagcagta
    20221 acgcgccgcc tgccagccac ggaacaaata catccggggt aatcaggcag aaaaagaaca
    20281 tcagaaagga ggcgacgatc agcaacgctt cgcgcagccc gcgcggacga ctcagcgcat
    20341 actcttcgtg cgtttctttg cgaatattga gtagctcgat ttgctcactc tcttccccgc
    20401 gaatcgacgc ttgcgtggac ggaacaggtt gcaaggcata gccgcgcgtt tcctgcatgt
    20461 actcctgcag cgtatgaccg ttgagtgaaa taaccagcgg cagcgaatcg gtatgaatca
    20521 gttcaacggt attctcatcg ttgatgtact gttcccagaa agggggcagg tggacttcta
    20581 ccgaatcgag gtagtaacgc catttattcg gatcgtcggt agagatgccg taacgcgtga
    20641 tagcgtgtgt cagcatcatg acgttgttgc tttcggcatt cagcgccaga gagatcggtg
    20701 ccgcgctggc tcccgttggg ccaggcacct gtaatacctg cgtcaggctc tcaagataat
    20761 tttcaacggc gctacgttct tcaggtgtga gtttacgcgt ttgcgcatcc gcgaaggcgt
    20821 tggtccaggg cagctgacgc cgtctggatc gcacttttat cagccatccc gcaagtagtg
    20881 agcaggccag caaagcagct aaaaaaatca caatggtgct catgctttcc ccatcttact
    20941 tagtctgtca ggcgtggtca gtcgcaccgg acacaataca tgattctgcg gttggctatc
    21001 ggcaagcgtg gagtttaact tgagcatcat taagcacatc acagtaaccg ctgatcatag
    21061 caaaccctct gaaggcggaa tatcagcaaa ttcctggagt tatttcaacc tattgacttt
    21121 taatttgatt tgaggacgat tatttattaa gttacataaa tgtaaataac atgcaattca
    21181 cattgccgaa agcgtgtccg atatcgcaca ataaccgctg atttcacagc atagatccca
    21241 cgatgagcaa atcattacaa aaacccacca ttctgaatgt tgaaactgta gcccgttccc
    21301 gactgtttac cgtcgagagc gtggatctgg agttcagcaa tggcgtgcgg cgtgtttatg
    21361 aacgaatgcg tccaaccaac cgggaagcag tgatgattgt gccgattgtg gacgatcacc
    21421 tgatcctgat ccgcgaatac gcagtgggaa ctgaatccta cgaattaggt ttttcgaaag
    21481 gattaattga tccgggtgaa agcgtctacg aagccgctaa ccgcgagcta aaagaagagg
    21541 ttggatttgg tgcgaacgat ctgacttttt tgaagaagct cagcatggca ccgtcttact
    21601 tttccagcaa aatgaatatc gtggtagcgc aagatctcta cccggaatcg ctggtaggcg
    21661 atgagccaga gccgctaccg caggtgcgct ggccgctggc gcatatgatg gatttgctgg
    21721 aagaccctga cttcaacgaa gcgcgtaatg tcagcgcgct gttcctcgtg cgcgaatggt
    21781 tgaaagggca ggggcgagtg taaatgcgtg aagtttgcct gatgcacaat gtttatcagg
    21841 catacggagt tcctgcaatt tattgagttt tcacgatttt gttggcctga taacgcgttc
    21901 acccgcatcc ggcataaaca aatgcacttt gtcagccaac tgaaaaggcg ccgaagcgcc
    21961 tttttaatca gaacaattcc tgtgcctcac cattatcgat aatggtcgtt cccacctcgt
    22021 gcaccgcctg ttgtgtcggc tgcgtacctt cgatgaaata ctcttcgcgg ctgttgccac
    22081 cattagctaa ctgcccggtg ctgcgatcga tattcaccgt cacaataccc ggaggcggcg
    22141 tcagcggctg ctccggcaca ccttcaagaa cggctttcat ataagcgtcc catgcaggct
    22201 gggcactctt ggcaccgcct tcgtaacctg agatctgatc tttaatcgct ccggaagccg
    22261 ttgtatgacc gagattacga cggtgatcat caaagccaat ccagaccgag gtcacaacgc
    22321 ccggaccgta acccgagaac cacgcatctt tcgaactgtt agtggtaccg gttttcccgc
    22381 cgatatcgcg acgctgcaaa tcacgacctg cacgccagcc ggtaccctgc cagcctggtt
    22441 caccaaagat attggtgttc aaagcactct taatcaggaa tgccagcgga gtgttgatga
    22501 cgtgcggtgc gtactcctgc gcgccagtct tcgccactaa cgcctgattt gcctgctcca
    22561 gctgcggcat tggtacagaa acattctgct gctcgcggga gatagcgaca tcttcaacat
    22621 cgttattttc cagcacgttc gatttctgcg tatcaccgta aatcaccgga atatcgcatt
    22681 ccgggcaggc tactttcggt ttcgcttcga aaatcacgcc gccctgatcg ttttcaattt
    22741 tgctgataaa ccacgggtcc accaggaagc caccgttcgc catgaccgcg tagccgcgcg
    22801 ccacctgcat tggggtgaag gacgctgaac ccagcgccag cgattcggtg tggacaatgt
    22861 tttgcgccgg gaagccgaag cgttgcagat attctgcagc gtagtcgacg cccatcgccc
    22921 gcattgcgcg taccatcacc acgtttttcg actgacccag cccctgacgt aagcgaattg
    22981 gaccagcata ctgcggtggt gagttcttcg gctgccagtc agaaccggca cctgcatccc
    23041 agcgagaaat tggcacatcg ttcaacatac ttgccagcgt cagaccttta tccatcgccg
    23101 cggtgtagag gaacggtttg atgttggaac ccacctgacg cagtgcctgg gtggcgcggt
    23161 taaacttgct ctgattgaaa tcaaagccac cgaccagcgc cataacggca ccgttttgcg
    23221 gattgatcga caccagcgcc gagttcactt ccggcacttg tgccagccac catgcatcgc
    23281 caacctgacg aacccagatt tgctgacccg tttgcagaac atcggtcact ttacgcggcg
    23341 tcggtccttg ctgagtatcc gaacggtaag gacgcgccca gcgaacgcct tccatactca
    23401 atgcgacggt cgacccgtcc gccagcatcg ccgtcgcttg ctgaggattg gcgctggtga
    23461 ctgcggcagg cagcagcgga ccataggttg gcagcgcctt cagcgtatcg gtaatcttgt
    23521 tgttatccca cgccgactcg cccactttcc acagcacatt tgccgggccg cgatagccgt
    23581 ggcgcatgtc gtagtccagc acgttattac gtaccgcctg ctgtgcggcc tgctgcactt
    23641 tgcgggtgat ggtggtgtaa atgcgataac cgtcttcata ggcactttcg ccataacggt
    23701 tatacatctc ctggcgcacc atttcgctca ggtacggcgc agagaaagca atctccggcg
    23761 cgtgatagtt agcgttaatc gcctcagtgc gtgtctgatc gaactgttgt tgggtgatat
    23821 acccttcatc cagcatccgc gacagtacga cgttacgccg cgcgacggca cgatccattg
    23881 agtagagcgg attaaaggtg gaaggcgctt tcggcagccc ggcgatcaca gccatttcgt
    23941 tcagcgtcag ttggtcgacc gttttaccga aatagacttg tgccgcagca ccgacaccat
    24001 aggcgcggta accgaggtaa atcttgttca gataaagctc gaggatctcg tctttcgtca
    24061 gcaactgttc aatgcgaatc gcgaggaaga cttccttaat cttacgcatc agcgtgcgtt
    24121 ctggactgag gaagaaattt ctcgccagct gctgggtaat ggtactcgcc ccttgtgacg
    24181 cgtggccgga gaacagcgcc acacttgccg cacggaagat ccccaccggg tcaacgccgt
    24241 gatgctcgta gaagcggctg tcttccgtcg cgataaaggc tttcaccatc tccggtggga
    24301 tttgatccaa cgtaaccgga atacgacgtt tctcaccgta ttgagcaatc agctcgccat
    24361 cggcgctgta aatctgcatc ggaatttgca ggcgaacatc ttttaatgtc gccacatccg
    24421 gcagttgtgg ctcgatgtag cggtataggc cataaatcga gcctgctccc agcaaaatgc
    24481 aacagactgc aaggatcaaa aaatacttta cgaacttcac tggaaatttc ccatttagtt
    24541 tcatttgggc agtttataaa caaacgcgcg gtagtataaa ggcaagccag acgcattgat
    24601 atacccgtca gagtgacggg tgataaggag atcatcacaa tggcatttaa gatctggcaa
    24661 attggtttgc atttacaaca gcaagaagcg gtagcggttg cgatcgtacg gggcgcaaaa
    24721 gaatgctttt tgcaacgctg gtggcggttg ccgctggaga acgacattat caaagatggg
    24781 cggattgttg atgcgcagca gctggctaaa acgttgttac cttggagtcg cgaactgccg
    24841 cagcgtcatc acattatgtt ggcgtttccc gccagtcgca cattacagcg gtcatttccg
    24901 cgcccgtcga tgtcccttgg tgagcgggag caaacggcct ggctgtcagg gacgatggcc
    24961 cgcgagctgg atatggatcc ggactccctg cgcttcgatt atagcgaaga ctcactcagc
    25021 cccgcttata acgtgactgc cgcgcaaagc aaagagctgg caacgctgct tactctggca
    25081 gaaaggttgc gtgttcatgt tagtgcgatc accccggatg ccagtgcatt acagcgattc
    25141 ctgccttttt taccttctca tcagcaatgt ctggcctggc gtgataacga acagtggctg
    25201 tgggcgacac gctatcgctg ggggcgcaaa ctggcggtgg ggatgactag cgcgaaggag
    25261 ctggcggcag cgttatccgt tgatcccgaa agcgtcgcga tatgtggcga agggggattt
    25321 gatccctggg aggccgtttc tgttcgtcag ccgccgctac cgccgtcagg tggagacttt
    25381 gccatcgcgc tggggctggc gcttgggaag gcgtactgat gaacccgcca attaattttt
    25441 tgccctggcg acagcaacgc cggaccgctt ttctgcgttt ctggttgctg atgttcgttg
    25501 cgcctctgct gctggccgtc gggataacgc taatactgcg tctgacaggc agcgccgaag
    25561 cgcgcataga cgcagttttg cttcaggcgg aacaacaact cgcccgcagc ttacagataa
    25621 cgaagccacg tttgctggag caacagcagt tacgcgaaca gcgttcgcaa cggcagcgcc
    25681 agcgacaatt tacccgcgac tggcaatctg cgctggaagc actggcggcg cttttacccg
    25741 agcacgcctg gctgacaacg ataagctggc agcagggaac gctggagatc aaggggctga
    25801 caacaagcat taccgcgtta aacgcactag aaacgtcact ccgccaggat gcgtcttttc
    25861 atctcaatca gcggggagcc acgcagcagg atgcgcaggg acgctggcaa tttgagtatc
    25921 agttaacaag gaaggttagc gatgaacatg ttctttgact ggtggttcgc cacatcaccc
    25981 cgcctccgcc agctttgctg ggcattctgg ttgctgatgt tagttacgct catttttcta
    26041 tcatcgacac accatgaaga gcgcgacgca ttaattcgac tacgggcaag tcatcaccag
    26101 cagtgggccg cactgtatcg cctggtagac accactccct tcagcgagga aaaaacgctg
    26161 cccttttcgc ctctggattt tcagttatcc ggcgcgcaac tggtttcctg gcatccatcc
    26221 gcgcagggag gtgagttggc gttgaaaacg ctgtgggaag cagtgccgtc ggcatttaca
    26281 cggctggcag agcgcaacgt cagcgtgagc cgtttttcgt taagcgtgga aggtgatgat
    26341 cttttgttca cgctacaact ggagacaccg catgagggtt aaacgctggt tgttggcagg
    26401 tattgcattg tgccttttaa ccggtatgcg tgaccctttt aaaccgccgg aagatctatg
    26461 ccggattagc gaacttagcc agtggcgcta tcaggggatg gtagggcgag gcgagcacat
    26521 catcggtgta ataaaagacg ggcaaaagaa atggcgacgg gtgcagcaaa acgatgtgct
    26581 ggaaaacggc tggacaattt tacagctgac gccagacgca ctaacgctgg gaaccgggac
    26641 aaactgcgaa ccgccacaat ggttgtggca acggcaagga gatacaaatg aagcaatgga
    26701 tagccgcact actgttgatg ctgatacccg gcgtacaggc ggcaaagccg caaaaagtga
    26761 cgctgatggt ggatgacgtt ccggtagctc aggtgttgca ggcgctggct gaacaggaga
    26821 agttgaacct ggtcgtgtcg ccagacgtca gcggtacggt gtcgttacat ctaacagatg
    26881 ttccctggaa gcaggcacta caaactgtag tgaaaagcgc cggactgata acgcggcagg
    26941 aaggcaacat tctctcagtg cattccattg cctggcagaa taacaatatc gcccgccagg
    27001 aggcggagca ggcgcgggcg caggcaaatc tgccgctgga aaatcgcagt ataaccctgc
    27061 aatacgccga cgcgggagaa ctggcgaaag cgggggagaa gctactgagt gccaaaggga
    27121 gtatgaccgt cgataaacgc accaatcgtc ttttgctacg agataacaaa acggcgttaa
    27181 gcgcgcttga acagtgggta gcgcaaatgg atctgccggt cgggcaggtt gagctgtcgg
    27241 cgcatattgt caccattaat gaaaaaagtt tgcgtgagtt aggcgtgaaa tggacgctgg
    27301 ccgatgcgca acacgctggt ggcgttgggc aagtcaccac gcttggtagc gacctctccg
    27361 tagcgacggc gacaacgcat gtcggtttta acattgggcg catcaacgga cgcttgctgg
    27421 atcttgagct ttccgcgctc gaacaaaaac agcagctgga tattatcgcc agtccgcgtc
    27481 tgctggcctc acatcttcag cctgccagca ttaaacaggg gagcgaaatt ccatatcagg
    27541 tttccagcgg ggaaagtggc gcgacgtcgg tggaatttaa agaggccgtc ctggggatgg
    27601 aggtcacgcc cacggtgtta caaaaaggtc gcatccggct gaaattacac atcagccaga
    27661 acgttccggg gcaggtgcta cagcaggccg atggcgaagt gctggcgatt gataagcagg
    27721 agatcgaaac gcaggtcgag gtcaaaagcg gagaaacgtt ggcgctgggc ggcattttta
    27781 cccgtaaaaa taaatcgggt caggatagcg taccgttgct tggcgacatt ccctggttcg
    27841 ggcaattatt tcgtcatgac ggaaaagaag atgaacgacg cgagttagtg gtgtttatca
    27901 cgccacgact ggtttccagt gagtaaacag ccgtaaaagc ggtaatgttt ttatgctgaa
    27961 cgtgtttcat ctatttgacg cgcgcaggta tttagcatac aaggagtacc gatttgagag
    28021 ttggtgctct tcgctgcctg cgttccatga tgatgattta tcattcaggc ggcattttgc
    28081 tgtctttttt acgctaatct tacccggtga tttatcgcca gagcggtggt agcaaggcag
    28141 cgcgcttgca gcgaccagat atgcagaggg atgggtgatt tattcagttg ccaaacccgc
    28201 tggagtattg agataatttt cagtctgact ctcgcaatat cttatgaggt ttcagttcat
    28261 gtcctgcggc gctctctgag cgaagcgggt ttatcattaa cgaatagtct tagtagtacc
    28321 gaaaaaatgg cagagaaacg caatatcttt ctggttgggc ctatgggtgc cggaaaaagc
    28381 actattgggc gccagttagc tcaacaactc aatatggaat tttacgattc cgatcaagag
    28441 attgagaaac gaaccggagc tgatgtgggc tgggttttcg atttagaagg cgaagaaggc
    28501 ttccgcgatc gcgaagaaaa ggtcatcaat gagttgaccg agaaacaggg tattgtgctg
    28561 gctactggcg gcggctctgt gaaatcccgt gaaacgcgta accgtctttc cgctcgtggc
    28621 gttgtcgttt atcttgaaac gaccatcgaa aagcaacttg cacgcacgca gcgtgataaa
    28681 aaacgcccgt tgctgcacgt tgaaacaccg ccgcgtgaag ttctggaagc gttggccaat
    28741 gaacgcaatc cgctgtatga agagattgcc gacgtgacca ttcgtactga tgatcaaagc
    28801 gctaaagtgg ttgcaaacca gattattcac atgctggaaa gcaactaatt ctggctttat
    28861 atacactcgt ctgcgggtac agtaattaag gtggatgtcg cgttatggag aggattgtcg
    28921 ttactctcgg ggaacgtagt tacccaatta ccatcgcatc tggtttgttt aatgaaccag
    28981 cttcattctt accgctgaaa tcgggcgagc aggtcatgtt ggtcaccaac gaaaccctgg
    29041 ctcctctgta tctcgataag gtccgcggcg tacttgaaca ggcgggtgtt aacgtcgata
    29101 gcgttatcct ccctgacggc gagcagtata aaagcctggc tgtactcgat accgtcttta
    29161 cggcgttgtt acaaaagccg catggtcgcg atactacgct ggtggcgctt ggcggcggcg
    29221 tagtgggcga tctgaccggc ttcgcggcgg cgagttatca gcgcggtgtt cgtttcattc
    29281 aagtcccgac gacgttactg tcgcaggtcg attcctccgt tggcggcaaa actgcggtca
    29341 accatcccct cggtaaaaac atgattggcg cgttctacca gcctgcttca gtggtggtgg
    29401 atctcgactg tctgaaaacg cttcccccgc gtgagttagc gtcggggctg gcagaagtca
    29461 tcaaatacgg cattattctt gacggtgcgt tttttaactg gctggaagag aatctggatg
    29521 cgttgttgcg tctggacggt ccggcaatgg cgtactgtat tcgccgttgt tgtgaactga
    29581 aggcagaagt tgtcgccgcc gacgagcgcg aaaccgggtt acgtgcttta ctgaatctgg
    29641 gacacacctt tggtcatgcc attgaagctg aaatggggta tggcaattgg ttacatggtg
    29701 aagcggtcgc tgcgggtatg gtgatggcgg cgcggacgtc ggaacgtctc gggcagttta
    29761 gttctgccga aacgcagcgt attataaccc tgctcaagcg ggctgggtta ccggtcaatg
    29821 ggccgcgcga aatgtccgcg caggcgtatt taccgcatat gctgcgtgac aagaaagtcc
    29881 ttgcgggaga gatacgctta attcttccgt tggcaattgg taagagtgaa gttcgcagcg
    29941 gcgtttcgca cgagcttgtt cttaacgcca ttgccgattg tcaatcagcg taacaacaag
    30001 aaaggtcagg ccgcttatca agcggtctat tagcttcagg ttaattgcaa cgtggtaagc
    30061 attaaccttt tagtggggtg ttaaatggat gaattcaaac cagaagacga gctgaaaccc
    30121 gatcccagcg atcgtcgtac tggtcgttct cgtcaatctt ctgaacgttc tgagcgtact
    30181 gaacgtggcg aaccgcagat caattttgat gatattgaac ttgatgacac tgacgatcgc
    30241 cgtccgactc gtgcgcaaaa agagcgcaat gaggaaccgg aaatcgaaga agaaattgac
    30301 gaatccgaag atgaaaccgt ggatgaagag cgcgtagagc gtcgtccgcg taagcgcaaa
    30361 aaagcagcca gtaaacccgc ttctcgtcag tatatgatga tgggcgtcgg cattctggtt
    30421 ctactgctgt tgatcatcgg tatcggttct gcgctaaaag ccccctcgac cacttccagc
    30481 gatcaaaccg cgtctggcga gaagagtatt gatcttgctg gcaatgcgac cgatcaggcg
    30541 aatggggtgc agccagcgcc gggaaccacg tctgcggaaa atactcagca ggatgtttct
    30601 ctgccgccga tctcttctac gccgactcaa gggcaaaccc cggcggcaac ggatggtcaa
    30661 caacgtgttg aagtgcaggg tgacctgaac aatgcgctga cccagccaca aaatcagcaa
    30721 ccgctgaaca atgtggcggt caattccacg ttgccgactg aacccgcgac tgtcgcgcct
    30781 gttcgcaatg gcaatgcatc gcgtgacacg gcgaaaacgc aaaccgctga acgtccggcc
    30841 actacacgcc cagctcgtca gcaggcggtg attgaaccga aaaaaccgca agcaaccgtg
    30901 aaaacggagc cgaagccggt agcacagacg ccgaaacgta ctgaaccagt tgctcctgtg
    30961 gcgagcacga aggcaccggc tgcgacttct gcgccagcac caaaagagac ggcgactacg
    31021 gctccagtac agacggcatc cccggcgcaa accacggcaa caccagccgc tggagggaag
    31081 accgcaggta atgttggttc gttgaaatcg gcaccgtcca gccattacac tctgcagctg
    31141 agcagttcct ctaactacga caacctgaac ggttgggcga agaaagagaa tctgaaaaac
    31201 tacgttgtct atgaaacgac gcgtaatggt cagccgtggt atgtcctggt ttctggcgtg
    31261 tacgcttcga aagaagaggc gaaaaaagcg gtatctacat tgccagcaga tgttcaggcc
    31321 aaaaacccgt gggcgaaacc gctgcgtcag gtacaggccg atctgaagta atcaaggtta
    31381 tctcccgcaa tgggttatcg ttgcgggagt tgcctgaagc gctggatgct gtcggagctt
    31441 tctccacagc cggagaaggt gtaattagtt agtcagcatg aagaaaaatc gcgctttttt
    31501 gaagtgggca gggggcaagt atcccctgct tgatgatatt aaacggcatt tgcccaaggg
    31561 cgaatgtctg gttgagcctt ttgtaggtgc cgggtcggtg tttctcaaca ccgacttttc
    31621 tcgttatatc cttgccgata tcaatagcga cctgatcagt ctctataaca ttgtgaagat
    31681 gcgtactgat gagtacgtac aggccgcacg cgagctgttt gttcccgaaa caaattgcgc
    31741 cgaggtctac tatcagttcc gcgaagagtt caacaaaagc caggatccgt tccgtcgggc
    31801 ggtactgttt ttatatttga accgctacgg ttacaacggc ctgtgtcgtt acaatctgcg
    31861 cggtgagttt aacgtgccgt ttggccgcta caaaaaaccc tatttcccgg aagcagagtt
    31921 gtatcacttc gctgaaaaag cgcagaatgc ctttttctat tgtgagtctt acgccgatag
    31981 catggcgcgc gcagatgatg catccgtcgt ctattgcgat ccgccttatg caccgctgtc
    32041 tgcgaccgcc aactttacgg cgtatcacac aaacagtttt acgcttgaac aacaagcgca
    32101 tctggcggag atcgccgaag gtctggttga gcgccatatt ccagtgctga tctccaatca
    32161 cgatacgatg ttaacgcgtg agtggtatca gcgcgcaaaa ttgcatgtcg tcaaagttcg
    32221 acgcagtata agcagcaacg gcggcacacg taaaaaggtg gacgaactgc tggctttgta
    32281 caaaccagga gtcgtttcac ccgcgaaaaa ataattctca aggagaagcg gatgaaacag
    32341 tatttgattg ccccctcaat tctgtcggct gattttgccc gcctgggtga agataccgca
    32401 aaagccctgg caactggcgc tgatgtcgtg cattttgacg tcatggataa ccactatgtt
    32461 cccaatctga cgattgggcc aatggtgctg aaatccttgc gtaactatgg cattaccgcc
    32521 cctatcgacg tacatctgat ggtgaaaccc gtcgatcgca ttgtgcctga tttcgctgcc
    32581 gctggtgcca gcatcattac ctttcatcca gaagcctccg agcatgttga ccgcacgctg
    32641 caactgatta aagaaaatgg ctgtaaagcg ggactggtat ttaacccggc gacaccgctg
    32701 agctatctgg attacgtgat ggataagctg gatgtgatcc tgctgatgtc cgtcaatcct
    32761 ggtttcggcg gtcagtcttt cattcctcaa acactggata aactgcgcga agtacgtcgc
    32821 cgtatcgacg agtctggctt tgacattcgt ctggaagtgg acggtggcgt gaaggtgaac
    32881 aacattggcg aaatcgctgc ggcgggcgcg gatatgttcg tcgccggttc ggcaatcttc
    32941 gaccagccag actacaaaaa agtcattgat gaaatgcgca gtgaactggc aaaggtaagt
    33001 catgaataag tttgaagata ttcgcggcgt cgcttttgat cttgatggta cgctggtcga
    33061 cagtgctccc ggtcttgctg ctgcggtaga tatggcgctg tatgcgctgg agttgcccat
    33121 cgcaggtgaa gaacgcgtta ttacctggat tggtaacggc gcagatgttc tgatggagcg
    33181 cgcattgacc tgggcgcgtc aggaacgtgc gactctgcgt aaaacaatgg gtaaaccgcc
    33241 cgttgatgac gacattccgg cagaagaaca ggtacgtatt ctgcgtaaac tgttcgatcg
    33301 ctactatagc gaggttgccg aagaggggac gtttttgttc ccgcacgttg ccgatacgtt
    33361 gggcgcgttg caggctaaag gcctgccgct aggcctggtc accaacaaac cgacgccgtt
    33421 cgtcgcgccg ctgctcgaag ccttagatat tgccaaatac tttagcgtgg ttatcggcgg
    33481 cgatgatgtg caaaacaaaa aaccgcatcc ggacccgctg ttactggtgg ctgagcggat
    33541 gggaattgcc ccacaacaga tgctgtttgt cggcgactca cgcaatgata ttcaggcggc
    33601 aaaagcggca ggttgcccat cagttggctt aacctacgga tataactacg gcgaggctat
    33661 cgatctcagc cagcctgatg taatttatca gtctataaat gaccttctgc ccgcattagg
    33721 gcttccgcat agcgaaaatc aggaatcgaa aaatgactaa gcccatcgtt tttagtggcg
    33781 cacagccctc aggtgaattg accattggta actacatggg tgcgctgcgt cagtgggtaa
    33841 acatgcagga tgactaccat tgcatttact gtatcgttga ccaacacgcg atcaccgtgc
    33901 gccaggatgc acagaagctg cgtaaagcga cgctggatac gctggccttg tatctggctt
    33961 gtggtatcga tcctgagaaa agcaccattt ttgttcagtc ccacgtacca gaacatgcgc
    34021 agttaggctg ggcactgaac tgctatacct acttcggcga actgagccgc atgacccagt
    34081 ttaaagataa atctgcgcgt tatgccgaga acatcaacgc tggtctgttt gactatccgg
    34141 tgctgatggc tgcggacatc ctgctgtatc aaactaatct ggtaccggtg ggtgaagacc
    34201 agaaacagca cctggaactg agtcgcgata tcgcccagcg tttcaacgcg ctgtatggcg
    34261 agatctttaa ggtgccggag ccgtttattc cgaaatctgg cgcgcgcgta atgtcgctgc
    34321 tggagccgac caagaagatg tccaagtctg acgataaccg caataacgtt atcggcctgc
    34381 tggaagatcc gaaatcggta gtgaagaaaa tcaaacgcgc ggtcactgat tctgacgagc
    34441 cgccggtagt tcgctacgat gtgcagaaca aagcgggcgt ttccaacctg ctggatatcc
    34501 tttctgcggt aacgggccag agcattccag aactggaaaa acagttcgaa ggcaagatgt
    34561 atggtcatct gaaaggtgaa gtggctgatg ccgtttccgg tatgctgact gaattgcagg
    34621 aacgctatca ccgtttccgc aacgatgaag ccttcctgca acaggtgatg aaagacggcg
    34681 cagaaaaagc cagtgtacac gcttcccgta cgctgaaagc ggtgtacgaa gcgattggtt
    34741 ttgtggcgaa gccgtaatat caaaacgctc
//
LOCUS       DAPHDW010000036        33635 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.036, whole genome shotgun sequence.
ACCESSION   DAPHDW010000036 DAPHDW010000000
VERSION     DAPHDW010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 33635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.036"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            8..235
                     /locus_tag="RH057_001729"
     CDS             8..235
                     /locus_tag="RH057_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HDV3128112.1"
                     /translation="MLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLLRGW
                     IYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            238..1095
                     /locus_tag="RH057_001730"
     CDS             238..1095
                     /locus_tag="RH057_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3128113.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEK
                     VSITPQVSGRIVELNIKDNQLVNAGDLLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFISAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LRGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQRVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            1095..3107
                     /locus_tag="RH057_001731"
     CDS             1095..3107
                     /locus_tag="RH057_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV3128114.1"
                     /translation="MNASSWSLRNLPWFKATLAQWRYALRNTIAMCLALTVAYYLNLD
                     EPYWAMTSAAVVSFPTVGGVISKSLGRIAGSLLGAIAALLLAGHTLNEPWFFLLSMSA
                     WLGFCTWACAHFTNNVAYAFQLAGYTAAIIAFPMVNITEASQLWDIAQARVCEVIVGI
                     LCGGMMMMILPSSSDATALLTALKNMHARLLEHASLLWQPETNDAIRAAHEGVIGQIL
                     TMNLLRIQAFWSHYRFRQQNARLNALLHQQLRMTSVISSLRRMLLNWPSPPGATREIL
                     EQLLTALASSQTDAYTVARIIAPLRPTNVADYRHVAFWQRLRYFCRLYLQSSQELHRL
                     QSGVDDHARLARTSALARHTDNAEAMWSGLRTFCTLMMIGAWSIASQWDAGANALTLA
                     AISCVLYSAVAAPFKSLSLLMRTLVLLSLFSFVVKFGLMVQISDLWQFLLFLFPLLAT
                     MQLLKLQMPKFAALWGQLIVFMGSFIAVTNPPVYDFADFLNDNLAKIVGVALAWLAFA
                     ILRPGSDARKSRRHIRALRRDFVDQLSRHPTLSESEFESLTYHHVSQLSNSQDALARR
                     WLLRWGVVLLNCSHVVWQLRDWESRSDPLSRVRDNCISLLRGVMSERGVQQKSLAATL
                     EELQRICDSLARHHQPAARELAAIVWRLYCSLSQLEQAPPQGTLAS"
     gene            complement(3108..3629)
                     /gene="sodC"
                     /locus_tag="RH057_001732"
     CDS             complement(3108..3629)
                     /gene="sodC"
                     /locus_tag="RH057_001732"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HDV3128115.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGAGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(3710..4606)
                     /locus_tag="RH057_001733"
     CDS             complement(3710..4606)
                     /locus_tag="RH057_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HDV3128116.1"
                     /translation="MVQRITIAPQGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RDHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQQLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(4655..4894)
                     /locus_tag="RH057_001734"
     CDS             complement(4655..4894)
                     /locus_tag="RH057_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HDV3128117.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            4997..5596
                     /gene="nemR"
                     /locus_tag="RH057_001735"
     CDS             4997..5596
                     /gene="nemR"
                     /locus_tag="RH057_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416166.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HDV3128118.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLTELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIDLLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            5633..6730
                     /gene="nemA"
                     /locus_tag="RH057_001736"
     CDS             5633..6730
                     /gene="nemA"
                     /locus_tag="RH057_001736"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HDV3128119.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMAVQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAGFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRISPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQRKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            6811..7218
                     /gene="gloA"
                     /locus_tag="RH057_001737"
     CDS             6811..7218
                     /gene="gloA"
                     /locus_tag="RH057_001737"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HDV3128120.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            7321..7968
                     /gene="rnt"
                     /locus_tag="RH057_001738"
     CDS             7321..7968
                     /gene="rnt"
                     /locus_tag="RH057_001738"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HDV3128121.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            8061..12677
                     /locus_tag="RH057_001739"
     CDS             8061..12677
                     /locus_tag="RH057_001739"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HDV3128122.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARS
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQLPLKGIADERRRRGETEVNLRVGIRTGDTPAQERSKLTRNPPDILITTPESLYL
                     MLTSRARETLRGVETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGLSATVR
                     SASDVAAFLGGDRPVTVVNPPAMRHPQIRIVVPVANMDDVSSVASSTGEDSHAGREGS
                     IWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVDAAHFE
                     STSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLELGIDMG
                     AVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVECMFAGR
                     LENLTPPHNPLDVLAQQTVAAAAMEALQVDEWYSRVRRAAPWKDLPRRVFDATLDMLS
                     GRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPEGEEKA
                     GSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSARLPFWRGEG
                     NGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATGIVPGS
                     RHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAITGRIHALWGADASVVASDDGIVA
                     RIPDTDGKLPDAAIFLFEPEKLLQIVREAVGSSALFAARFRECAARALLMPGRTPGHR
                     TPLWQQRLRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQIS
                     DVTTTTPSPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELL
                     DPQVIRQVEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLE
                     NLLAVKRIFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLR
                     YLRAHALVTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRS
                     LQAAREATRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQL
                     AGVGLPASLWESQILPARVRDYSSEMLDELLATGAVIWSGQKKLGEDDGLVALHLQEY
                     AAESFTPAEADQANRSALQQAIVAVLADGGAWFAQQISQRIRDKIGESVDLSALQEAL
                     WALVWQGVITSDIWAPLRALTRSSSNARTSTRRSHRARRGRPVYAQPVSPLVSYNTPN
                     LAGRWSLLQVEPLNDTERMLALAENMLDRYGIISRQAVIAENIPGGFPSMQTLCRSME
                     DSGRIMRGRFVEGLGGAQFAERLTIDRLRDLATQATQTRHYTPVALSANDPANVWGNL
                     LPWPAHPATLVPTRRAGALVVVSGGKLLLYLAQGGKKMLVWQEKEELLAPEVFHALTT
                     ALRREPRLRFTLTEVNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(12728..13075)
                     /gene="grxD"
                     /locus_tag="RH057_001740"
     CDS             complement(12728..13075)
                     /gene="grxD"
                     /locus_tag="RH057_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HDV3128123.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            13410..14225
                     /gene="mepH"
                     /locus_tag="RH057_001741"
     CDS             13410..14225
                     /gene="mepH"
                     /locus_tag="RH057_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HDV3128124.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKTAKKAASKSTTKSKTASSVKKSSITASKNAKTRSKHAVNKTASA
                     SFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPYRWGG
                     SSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAAPIERSELKNGDLVFFRTQG
                     RGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            14353..14934
                     /gene="sodB"
                     /locus_tag="RH057_001742"
     CDS             14353..14934
                     /gene="sodB"
                     /locus_tag="RH057_001742"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HDV3128125.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(15080..16249)
                     /locus_tag="RH057_001743"
     CDS             complement(15080..16249)
                     /locus_tag="RH057_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3128126.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGAGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMRVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVMGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(16415..16504)
                     /gene="ynhF"
                     /locus_tag="RH057_001744"
     CDS             complement(16415..16504)
                     /gene="ynhF"
                     /locus_tag="RH057_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HDV3128127.1"
                     /translation="MSTDLKFSLVTTIIVLGLIVAVGLTAALH"
     gene            16803..17828
                     /gene="purR"
                     /locus_tag="RH057_001745"
     CDS             16803..17828
                     /gene="purR"
                     /locus_tag="RH057_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HDV3128128.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(17825..18757)
                     /locus_tag="RH057_001746"
     CDS             complement(17825..18757)
                     /locus_tag="RH057_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3128129.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     VVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLGDSETLNKEWLREPEETPATGD"
     gene            18870..20081
                     /locus_tag="RH057_001747"
     CDS             18870..20081
                     /locus_tag="RH057_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HDV3128130.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVGCQNH
                     GNAEVAHSESH"
     gene            20372..21520
                     /gene="cfa"
                     /locus_tag="RH057_001748"
     CDS             20372..21520
                     /gene="cfa"
                     /locus_tag="RH057_001748"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HDV3128131.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIASAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCEGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(21560..22201)
                     /gene="ribE"
                     /locus_tag="RH057_001749"
     CDS             complement(21560..22201)
                     /gene="ribE"
                     /locus_tag="RH057_001749"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HDV3128132.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            22416..23789
                     /gene="mdtK"
                     /locus_tag="RH057_001750"
     CDS             22416..23789
                     /gene="mdtK"
                     /locus_tag="RH057_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HDV3128133.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRSMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPDPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRLPSVI
                     ILQRASR"
     gene            complement(23830..25086)
                     /locus_tag="RH057_001751"
     CDS             complement(23830..25086)
                     /locus_tag="RH057_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV3128134.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVIGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPGGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGADLQQSTITVQQGGVLILDGSTVKGDGVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVTGDIHVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            25394..25470
                     /locus_tag="RH057_001752"
     tRNA            25394..25470
                     /locus_tag="RH057_001752"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25428..25430,aa:Val,seq:gac)
     gene            25475..25551
                     /locus_tag="RH057_001753"
     tRNA            25475..25551
                     /locus_tag="RH057_001753"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25509..25511,aa:Val,seq:gac)
     gene            25659..25964
                     /locus_tag="RH057_001754"
     CDS             25659..25964
                     /locus_tag="RH057_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HDV3128135.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            26090..27694
                     /locus_tag="RH057_001755"
     CDS             26090..27694
                     /locus_tag="RH057_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
                     /protein_id="HDV3128136.1"
                     /translation="MKKIAIVGAGPTGIYTLFSLLQQQTPLSISIFEQADEAGVGMPY
                     SDEENSKMMLANIASIEIPPINCTYLEWLQKQEASHLQRYGVKKETLHDRQFLPRILL
                     GEYFRDQFLRLVDQARQQKFAVAVYESCQVTDLQITNAGVMLATNQDLPSETFDLAVI
                     ATGHVWPDEEEATRTYFPSPWSGLMEAKVDACNVGIMGTSLSGLDAAMAVAIQHGSFI
                     EDDKQHVVFNRDNASEKLNITLMSRTGILPEADFYCPIPYEPLHIVTDQALNAEIQKG
                     EEGLLDRVFRLIVEEIKFADPDWSQRIALESLNVDSFAQAWFAERKQRDPFDWAEKNL
                     QEVERNKREKHTVPWRYVILRLHEAVQEIVPHLNEHDHKRFSKGLARVFIDNYAAIPS
                     ESIRRLLALREAGIIHILALGEDYKMEINESRTVLKTEDNSYSFDVFIDARGQRPLKV
                     KDIPFPGLREQLQKTGDEIPDVGEDYTLQQPEDIRGRVAFGALPWLMHDQPFVQGLTA
                     CAEIGEAMARAVVKPASRARRRLSFD"
     gene            complement(27706..28518)
                     /locus_tag="RH057_001756"
     CDS             complement(27706..28518)
                     /locus_tag="RH057_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128137.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIHHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERALVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGPGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(28522..29307)
                     /locus_tag="RH057_001757"
     CDS             complement(28522..29307)
                     /locus_tag="RH057_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HDV3128138.1"
                     /translation="MNSSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(29304..29972)
                     /locus_tag="RH057_001758"
     CDS             complement(29304..29972)
                     /locus_tag="RH057_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HDV3128139.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYQVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(30036..30674)
                     /locus_tag="RH057_001759"
     CDS             complement(30036..30674)
                     /locus_tag="RH057_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HDV3128140.1"
                     /translation="MNHRDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARAVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(30687..32789)
                     /locus_tag="RH057_001760"
     CDS             complement(30687..32789)
                     /locus_tag="RH057_001760"
                     /EC_number="1.2.7.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016246021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
                     /protein_id="HDV3128141.1"
                     /translation="MANGWTGNILRVNLTTGNITLEDSSKFKSFVGGMGFGYKIMYDE
                     VPPGTKPFDEANKLVFATGPLTGSGAPCSSRVNITSLSTFTKGNLVVDAHMGGFFAAQ
                     MKFAGYDVIIIEGKAKSPVWLKIKDDKVSLEKADFLWGKGTRATTEEICRLTSPETCV
                     AAIGQAGENLVPLSGMLNSRNHSGGAGTGAIMGSKNLKAIAVEGTKGVNIADRQEMKR
                     LNDYMMTELIGANNNHVVPSTPQSWAEYSDPKSRWTARKGLFWGAAEGGPIETGEIPP
                     GNQNTVGFRTYKSVFDLGPAAEKYTVKMSGCHSCPIRCMTQMNIPRVKEFGVPSTGGN
                     TCVANFVHTTIFPNGPKDFEDKDDGRVIGNLVGLNLFDDYGLWCNYGQLHRDFTYCYS
                     KGVFKRVLPAEEYAEIRWDQLEAGDVNFIKDFYYRLAHRVGELSHLADGSYAIAERWN
                     LGEEYWGYAKNKLWSPFGYPVHHANEASAQVGSIVNCMFNRDCMTHTHINFIGSGLPL
                     KLQREVAKELFGSEDAYDETKNYTPINDAKIKYAKWSLLRVCLHNAVTLCNWVWPMTV
                     SPLKSRNYRGDLALEAKFFKAITGEEMTQEKLDLAAERIFTLHRAYTVKLMQTKDMRN
                     EHDLICSWVFDKDPQIPVFTEGTDKMDRDDMHASLTMFYKEMGWDPQLGCPTRETLQR
                     LGLEDIAADLAAHNLLPV"
     gene            complement(32810..33436)
                     /locus_tag="RH057_001761"
     CDS             complement(32810..33436)
                     /locus_tag="RH057_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HDV3128142.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
BASE COUNT         8065 a         8884 c         8293 g         8393 t
ORIGIN      
        1 gaagtttatg ctcaatgcaa caggattgcc cttacaagat ctggtgttcg gtgcgtccgt
       61 ctattttcct ccgtttttca aagccttcgc gtttggattc gtcatctggc ttgtcgtaca
      121 ccgcctgctt cgtggctgga tctacgccgg tgacatctgg catcccttgt taatggattt
      181 atcgctgttt gcgatttgcg tttgccttgc tctggcaata ctgattgcgt ggtaactatg
      241 tcaattaaaa caattaagta tttctcaaca atcattgtag cgatagttgc ggttcttgcc
      301 ggatggtggc tgtggaatta ttacatgcaa tctccgtgga cacgcgacgg gaaaatacgc
      361 gcagaaaagg tttctatcac cccacaggtg tcgggacgta ttgttgagct gaatataaaa
      421 gacaaccaac tggttaacgc gggggatctt ctgctcacga tcgataaaac gccctttcag
      481 atcgccgagc tgaacgcaca ggctcagctg gcgaaagcgc aatctgacct cgcaaaagcc
      541 aataacgaag cgaatcgtcg tcgtcatctc tcacaaaatt ttatttctgc cgaagaactc
      601 gataccgcta acctcaatgt taaagcgatg caggccagtg ttaatgccgc acaggccacg
      661 ctgaaacaga cacaatggca actggcgcaa acggaaattc gcgccccggt gagtggttgg
      721 gtgactaacc tcactacccg catcggcgat tacgctgaca ccgggaaacc gctgtttgcc
      781 cttgtcgaca gccactcgtt ttacgtcatt ggttattttg aagaaaccaa gttgcgccat
      841 atccgcgaag gtgcaccagc acaaattacc ctctatagtg ataacaaaac gttacggggt
      901 cacgtttcca gtatcggtcg ggcgatttat gatcaaagcg ttgaaagtga ctcaagcctt
      961 attccggatg tgaaacctaa cgtcccctgg gttcgtctcg cccaacgcgt tcccgtccgc
     1021 tttgcgctgg ataaagttcc aggcgatgtc acgctggtgt cgggcaccac ctgtagcatt
     1081 gccgtaggtc aataatgaac gcatcgtcat ggtccttgcg caatttgccc tggttcaagg
     1141 ccacgctggc gcaatggcgt tatgcgttac gcaataccat tgccatgtgt ctggcgctga
     1201 cggttgccta ttatttgaat ctggatgaac cctattgggc gatgacctcg gctgcagtgg
     1261 ttagctttcc caccgttggc ggtgtcatca gcaaaagcct cggacgcatc gctggcagtt
     1321 tactgggagc cattgcggca ctgcttcttg ccgggcatac gctcaatgag ccgtggtttt
     1381 ttctattgag catgtcggcg tggcttggct tttgtacctg ggcctgtgcg cacttcacga
     1441 ataacgtcgc gtatgcattt caactggcgg gctacacggc tgccatcatc gcctttccga
     1501 tggttaatat tactgaggcc agccagctgt gggatatcgc tcaggcgcgc gtttgcgagg
     1561 tgatagtcgg tattttgtgc ggcggaatga tgatgatgat cctgcctagc agttccgatg
     1621 ctacagccct tttaaccgca ttgaaaaaca tgcacgcccg attactggaa catgccagtt
     1681 tactctggca gcctgaaaca aacgatgcca ttcgtgcagc acatgagggg gtgattgggc
     1741 agatactgac catgaatttg ctgcgtatcc aggctttctg gagccactat cgttttcgcc
     1801 agcaaaacgc gcgccttaat gcgctactcc accagcaatt acgtatgacc agtgtcatct
     1861 ccagcctgcg acgtatgttg ctcaactggc cctcaccgcc aggtgccacg cgagaaattc
     1921 tcgaacaatt gctgacggcg ctcgccagtt cgcaaacaga tgcttacacc gtcgcacgta
     1981 ttatcgcccc gctacgcccg accaacgtcg ccgactaccg gcacgtcgcc ttctggcagc
     2041 gactacgtta tttttgccgc ctttatctgc aaagtagtca ggaattacat cgtctgcaaa
     2101 gcggtgtaga tgatcatgcc agactcgcac ggacatccgc tctggctcgt cataccgaca
     2161 acgccgaagc tatgtggagc gggctgcgta cattttgtac gttgatgatg attggcgcat
     2221 ggagtattgc atcgcaatgg gatgccggtg ccaatgcatt aacgctggca gcaattagct
     2281 gcgtactcta ctccgccgtc gcagcaccgt ttaagtcgtt gtcactgctg atgcgcacgc
     2341 tggtgttact ttcgctattc agctttgtgg tcaaatttgg cctgatggtc cagattagcg
     2401 atctgtggca atttttactg tttctctttc cactgctggc gacaatgcag cttcttaaat
     2461 tgcagatgcc caaatttgcc gcactgtggg ggcaactgat tgtttttatg ggttcgttta
     2521 tcgctgtcac taatcccccg gtgtatgatt ttgctgattt tcttaacgat aatctggcaa
     2581 aaatcgttgg cgttgcgctg gcttggctag cgttcgccat tctgcgtcca ggatcggatg
     2641 ctcgtaaaag ccgccgccat attcgcgcgc tgcgccggga ttttgtcgat cagctaagcc
     2701 gccatccaac actgagtgaa agcgaatttg aatcgctcac ttatcatcac gtcagtcagt
     2761 tgagtaacag ccaggatgcg ctggctcgcc gttggttatt acgctggggt gtagtgctgc
     2821 tgaactgttc tcatgttgtc tggcaattgc gcgactggga atcgcgttcc gatccgttat
     2881 cgcgagtacg ggataactgt atttcactgt tgcggggagt gatgagtgag cgtggcgttc
     2941 agcaaaaatc actggcggcc acacttgaag aattacagcg gatttgcgac agccttgccc
     3001 gtcatcatca acctgccgcc cgtgagctgg cggcaattgt ctggcggctg tactgctcgc
     3061 tttcgcaact tgagcaagca ccaccgcaag gtacgctggc ctcgtaatta cttaattaca
     3121 ccacaggcat agcgttcacc gccaccgccc agcggtttag gttgatcgga catattatcg
     3181 ccgccaacgt ggaccatcag cgctttgtct ttgatttcat ccagtgattt cagacgaggc
     3241 gcgatgacgg catcggtagc tttgccgtca ttattgacga ccagtgcagg cagatcgcct
     3301 aaatgcccgg caccttctgg cccttcatgt ttaccggtat tttgtggatc aagatgcccg
     3361 cctgcggatt ccgcggcgct ggctttgcca tctttggtgg ctggctggca gcttcctttg
     3421 gcatgaatat ggaagccatg ttcaccgggg ggtaatgctt tcagatcggg cgaaaactcc
     3481 agacctttat cggtttcagt aatggtgacg ctaccaattg actgccctac cccttgcgac
     3541 gtgacgaggt tcatctcgac tttttcactg gcagcttgtg cgccggttgc aacaaccagc
     3601 gccagaatag ccagactaaa acgtttcata ggacctccgt tcattgtgca ttcctgaata
     3661 agtgtaaacc agtgacacat ttttgaacgg cggctattcc taaaagtgct tacggtacgt
     3721 cgtaccccag tgccgcttta cggatacgaa accattgttg acgggtcatt ttcagtgttt
     3781 ctgcttcgac agctgcccgt acgcgctcaa ttttacctga accgataatt ggcagcggct
     3841 gcgatggtaa acgtaatacc caggcgtaaa ccacctgttc aatcgagccc gcgtttaact
     3901 cctctgccac cacagccagt tcatcacgca gctgctggaa ataatcatca ttaaacagac
     3961 gaccaccacc aaggcaggac cacgccatcg gacgaacacg cagttgttgt agttggtcga
     4021 gcgtgccatc cagcagtaac ggctgatgca ccggggatat ttccacctga ttagtggcaa
     4081 gggtaaacgg cagacgtgat tgcaacaggg caaattgcgc aggcgtaaag ttcgatacgc
     4141 caaaatgacg cactttgccg ctctgatgca gatgtttgaa cgcgtccgcc acttcatcgg
     4201 catccattaa cgggtctggt cggtggatta acagcagatc cagatgatcg gtcgcgagat
     4261 taattagcga ctgttcggcg ctcttaatga tgtgatcgcg gtcagtgatg taatgaccaa
     4321 tgacgttttc ttcacgcgcg gtcgtcgcga taccgcattt actgacgatt tccatccgtt
     4381 cacgcaggtg aggtgccagt ttcagtgcct cgccaaacgc cgcttcgcac tgatagccac
     4441 cataaatatc agcatggtcc acggtggtca cgccgagatc cagatgctct tcaataaaac
     4501 tgaccagctg gcgggcggac atattccagt ccatcaatcg ccagtagccc atcacaaaac
     4561 gggaaaactc cgggccttgc ggcgcaatag taatacgctg aaccataatc gcttcctctt
     4621 atcagatatg agaggagtat acgcaagatt aggttcaaaa gagtgatggt tgctccggtt
     4681 cgtctgatga cgctggctta tttgcgcgta atttgcgcat taatcgctgc cgacaaaggc
     4741 gcagcacctc ttgtttttcg ccatcgctca ttttattcca gttaaaacgc tcatcccgac
     4801 tacgaaaaca gccgcgacaa aacccgcgtt cgtcagactg gcaaattccc cggcacgggc
     4861 tctggacggg aaagaactct aattgctccg ccacttcgcc ctcctcagat aagattatta
     4921 ccattattga agctgttaat gtccaaagta gcaactttgc ttgcactaga ccgactggtc
     4981 tactacactc caacgcatga acaaacacac cgaacatgat actcgcgaac atctcctggc
     5041 gacgggcgag caactttgcc tgcaacgtgg attcaccggg atggggctaa gcgaattact
     5101 aaaaaccgct gaagtgccga aagggtcctt ctatcactac tttcgctcta aagaagcgtt
     5161 tggcgttgcc atgcttgagc gtcattacgc cgcatatcac cagcgactga ctgagttgct
     5221 gcaatccggc gaaggtaact accgcgaccg catactggct tattaccagc aaacactgaa
     5281 ccagttttgc caacatggaa ccatcagtgg ttgcctgaca gtaaaactct ctgccgaagt
     5341 gtgcgatctg tcagaagata tgcgcagcgc gatggataaa ggtgctcgcg gcgtgatcga
     5401 cctgctctct caggcgctgg aaaatggccg tgagaaccat tgtttaacct tttgtggcga
     5461 accgctacaa caggcacaag tgctttacgc actatggctg ggtgcgaatc tgcaggccaa
     5521 aatttcgcgc agtttcgagc cactggaaaa cgcgctggcc catgtaaaaa acattattgc
     5581 gacgcctgcc gtttagcagg cattttttat caccagacga ccgggagcct ttatgtcatc
     5641 tgaaaaactg tattccccac tgaaagtggg cgcgatcacg gcggcaaacc gtatttttat
     5701 ggcaccgctg acgcgtctgc gcagtattga accgggtgac attcctaccc cgttgatggc
     5761 ggaatactat cgccaacgtg ccagtgccgg tttgattatt agtgaagcca cgcaaatttc
     5821 tgcccaggca aaaggatatg caggtgcgcc tggcatccat agtccggagc aaattgccgc
     5881 atggaaaaaa atcaccgctg gcgttcatgc tgaaaatggt catatggccg tgcagctgtg
     5941 gcacaccgga cgcatttctc acgccagcct gcaacctggc ggtcaggcac cggtagcgcc
     6001 ttcagcactt agcgcgggaa cacgtacttc tctgcgcgat gaaaatggtc aggcgatccg
     6061 tgttgaaaca tccatgccgc gtgcgcttga actggaagag attccaggta tcgtcaatga
     6121 tttccgtcag gccattgcta acgcgcgtga agccggtttt gatctggtag agctccactc
     6181 tgctcacggt tatttgctgc atcagttcct ttctccttct tcaaaccatc gtaccgatca
     6241 gtacggcggc agcgtggaaa atcgcgcacg tctggtactg gaagtggtcg atgccgggat
     6301 tgaagaatgg ggtgccgatc gcattggcat tcgcatttcg ccaatcggta ctttccagaa
     6361 cacggataac ggcccgaatg aagaagccga tgcactgtat ctgattgaac aactgggtaa
     6421 acgcggcatt gcttatctgc atatgtcaga accagattgg gcggggggtg aaccgtatac
     6481 tgatgcgttc cgcgaaaaag tacgcgcccg tttccacggt ccgattatcg gcgcaggtgc
     6541 atacacagta gaaaaagctg aaacgctgat cggcaaaggg ttaattgatg cggtggcatt
     6601 tggtcgtgac tggattgcga acccggatct ggtcgcccgc ttgcagcgca aagctgagct
     6661 taacccacag cgtgccgaaa gtttctacgg tggcggcgcg gaaggctata ccgattaccc
     6721 gacgttgtaa tccaacattg cgagcggcgt aaagccgccg ctatactaaa acaacatttt
     6781 gaatctgtta gccattttga ggataaaaag atgcgtcttc ttcataccat gctgcgcgtt
     6841 ggcgatctgc aacgctccat cgatttttat accaaagtgc tgggcatgaa actgctgcgt
     6901 accagcgaaa acccggaata caaatactca ctggcgtttg ttggctacgg cccggaaacc
     6961 gaagaagcgg tgattgaact gacctacaac tggggcgtgg ataaatacga actcggcact
     7021 gcttatggtc acatcgcgct tagcgtagat aacgccgctg aagcgtgcga aaaaatccgt
     7081 caaaacgggg gtaacgtgac ccgtgaagcg ggtccggtaa aaggcggtac tacggttatc
     7141 gcgtttgtgg aagatccgga cggttacaaa attgagttaa tcgaagagaa agacgccggt
     7201 cgcggtctgg gcaactaatc tcctgccggg cgtgaactca tcgcgcccgc atctttactg
     7261 catcgacaag taatatttgt cataatgcgc gctgcaattt atccgtatta agagaatcag
     7321 atgtccgata acgctcaact taccggtctg tgcgaccgtt ttcgtggttt ttatcctgtt
     7381 gtgatcgatg ttgaaacagc cggatttaac gccaaaaccg atgcgctgct tgagattgcc
     7441 gccatcaccc tgaaaatgga tgaacaaggc tggctgatgc cggacaccac attacatttc
     7501 cacgtcgaac catttgtcgg cgcaaatttg caaccagaag ccctcgcctt caacggcatt
     7561 gacccgaacg atcccgatcg cggcgcggtc agcgaatacg aggcgctgca cgaaattttt
     7621 aaagttgtac gtaaaggtat taaagcgagc ggctgtaacc gcgccattat ggtggcgcac
     7681 aatgccaatt ttgatcacag ctttatgatg gccgccgcag aacgcgcctc actgaaacgt
     7741 aacccgttcc accctttcgc cacttttgac actgctgcac tggccgggct ggcactcgga
     7801 caaaccgtat tgtcaaaggc ttgccagacc gctggcatgg acttcgacag cacccaggcg
     7861 cactccgcgc tgtacgacac cgaacgcact gctgtgctgt tttgtgaaat cgtcaaccgc
     7921 tggaaacgtc tgggaggctg gccgctacct gccgccgaag aggtgtaatc gagtcgatgc
     7981 ctgatgacat gcaatgattc aggcatctat agtgaggcta ttccacgcat cctgcatgat
     8041 attcacgggg aatagcgtta atggcagata atccagaccc ttcatcgctc ctgccggacg
     8101 tgttttcacc ggcgacccgc gactggtttc ttcgcgcctt taaacagccg accgctgtcc
     8161 agtcgcaaac ctggcatgtg gcggcgcgaa gcgaacatgc gctggtgatt gcaccgaccg
     8221 gctccgggaa aacgctggca gcatttctct acgccctcga tcggctcttc cgcgaaggcg
     8281 gcgaagatac ccgcgaggcg cataagcgta aaacctcacg catcctctat atttcaccga
     8341 taaaagctct gggcaccgac gttcagcgca acttgcagct cccgttgaag ggtattgccg
     8401 atgaacggcg gcggcgcggc gaaacggaag tcaatcttcg cgtagggatc cgtactggcg
     8461 atacgcctgc acaggaacgc agcaaactca cccgtaatcc gccggatatt ctgatcacca
     8521 cacccgaatc actctatctg atgctgacct cccgcgcgcg cgaaacgcta cgcggcgtcg
     8581 aaacggtaat tattgatgaa gtccacgcgg tagcgggcag taaacgtggt gcgcatctgg
     8641 cgttaagtct ggagcggctc gatgcgctgc tccacacctc agcacagcga attggccttt
     8701 ctgccactgt gcgctcagcc agcgatgtgg cagcatttct tggtggcgat cgcccggtta
     8761 cggtagtcaa cccgcccgca atgcgccatc cgcagatacg aattgtcgta ccggtcgcca
     8821 atatggatga tgtctcatcg gtcgccagca gcaccggcga agacagccat gccggccggg
     8881 aaggctccat ctggccatat attgaaacgg gtatccttga tgaagtgttg cgccatcgct
     8941 cgaccattgt ctttactaat tcacgtgggc tggcggaaaa actgacggca cgattaaatg
     9001 agctttacgc cgcacgctta cagcgttccc cgtctatcgc cgttgatgcg gcccatttcg
     9061 agtcgacctc cggcgcaacc tctaaccgtg tacaaagtag cgacgttttt attgcccgct
     9121 cacaccacgg ctccgtctct aaagaacaac gagcaatcac cgagcaggcg ctgaaatcgg
     9181 gtgagttacg ctgcgtggtc gcaacctcca gtctcgaact ggggattgat atgggcgcag
     9241 tggatctggt gattcaggtg gcaacgccgc tttctgttgc cagtgggtta caacgcattg
     9301 gtcgcgccgg acatcaggtt ggcggtgtat ctaaagggct gtttttccct cgtacccggc
     9361 gtgacttagt cgattccgca gtcattgtag agtgtatgtt cgcaggcagg ctggaaaacc
     9421 tgacaccgcc gcataatcca ctcgacgtcc ttgcgcagca aaccgttgcc gccgcggcga
     9481 tggaggcatt acaggtagac gaatggtact cccgcgtacg ccgtgccgca ccgtggaaag
     9541 atctgccaag acgtgttttt gacgccacgc tggatatgct ttccgggcgc tatccctctg
     9601 gcgatttttc tgcttttcgc cccaaactgg tctggaacag ggagaccggg atattgaccg
     9661 cccgacctgg cgctcaattg ttggcggtta ccagcggcgg caccattccg gatcgtggca
     9721 tgtatagcgt gttattaccc gaaggtgaag aaaaggccgg ttcgcggcgg gtgggtgaac
     9781 tggatgagga gatggtatat gagtcgcggg tgaacgacat tatcactctc ggcgctacct
     9841 catggcggat ccagcaaatc acccgcgatc aggtgattgt gactcctgct ccgggtcgtt
     9901 ctgcccggct ccccttctgg cgtggtgaag gtaacggacg tccggctgaa ttaggcgaga
     9961 tgatcggcga ttttcttcat ttgctggcgg atggcgcgtt cttttccggg actattcccc
    10021 cgtggctggc agaagaaaat acgaacgcca atattcaggg gttgattgac gagcaacgca
    10081 acgcgacggg catcgttccg gggagtcgcc atctggtcct cgaacggtgc cgtgatgaaa
    10141 ttggcgactg gcgtattatt ttgcactctc cctatggaag acgggtgcat gaaccctggg
    10201 cgctggcgat taccgggcga atacatgccc tatggggcgc tgacgcgtct gtggtcgcca
    10261 gtgatgacgg cattgttgcg cgcattcctg acactgatgg taaattgccc gacgccgcga
    10321 tttttttgtt tgaaccagaa aagttgctgc aaattgtccg cgaggcggta ggcagctcgg
    10381 cacttttcgc cgcccgtttt cgcgaatgcg ccgcgcgggc attattaatg ccggggcgca
    10441 ctccgggcca tcgcaccccg ctttggcaac aacggctgcg cgccagtcag ttgctggaaa
    10501 tcgctcaggg atatccggat tttccggtca ttctcgaaac cctgcgcgaa tgtctgcaag
    10561 atgtttatga tcttcccgca ctggaacgtt tgatgcgtcg cctgaacggt ggcgaaattc
    10621 aaatatccga tgtaacgacc actacgccct cgcctttcgc cacaagttta ttgttcggct
    10681 atgtcgcgga atttatgtac cagagcgacg ccccgctggc agagcgccgg gcatccgtac
    10741 tgtcgctgga cagcgagtta ctgcgcaatc tactcggaca ggtcgatccg ggggaattac
    10801 tcgacccgca ggtcattcgc caggtggaag aagagttgca acgactggct cctggcagaa
    10861 gagcgaaagg tgaagaagga ttgttcgacc tgctgcgcga actggggcca atgaccgttg
    10921 aagacctggc gcaacggcat acaggcagca gtgaagaggt tgcgtcgtat ctggaaaatc
    10981 ttcttgcagt aaaacgaatc ttcccagcga tgattagcgg acaggagcgt cttgcctgta
    11041 tggatgatgc cgccaggctg cgtgatgccc tcggcgtacg actaccagag tcattgccag
    11101 agatttattt acatagagtc agttacccgc ttcgcgacct ctttctgcgc tatctccggg
    11161 ctcatgctct ggtcacggct gaacaactgg ctcatgagtt tagtctcggt attgccattg
    11221 tcgaagagca gcttcagcaa ctgcgtgaac agggtctggt gatgaatctg caacaagaca
    11281 tctgggtgag cgatgaagta tttcgtcgtc tgcgtttgcg ctcgctgcaa gccgccagag
    11341 aagcgacgcg tcccgttgca gccacgacct atgcgcgatt gctgctggaa cgtcagggcg
    11401 tattacccgc caccgatggt agcccggcgc tctttgcctc aacatcgcca ggcgtttatg
    11461 agggcgtaga tggcgtgatg cgggtgatcg aacagcttgc cggagtcggt ttacccgcct
    11521 cactctggga aagccagatc ctgcctgccc gcgtacgcga ctattcgtca gaaatgctcg
    11581 atgaattact ggcaaccggt gcagttatct ggtcggggca aaaaaaactg ggtgaagatg
    11641 acggcctggt ggcactgcat ctacaggaat atgctgcaga atcgttcact cccgccgaag
    11701 cggatcaggc gaatcgttcg gcgctgcaac aagcgatagt cgctgttctg gctgacggag
    11761 gagcctggtt tgcacaacaa atcagccaac ggatacgcga caaaatcggc gaatcggttg
    11821 atctctctgc cctgcaagag gcgttatggg cgctggtctg gcaaggcgtc atcaccagcg
    11881 acatttgggc accgttacgc gccctcaccc gcagcagttc caacgcacgc acctcaactc
    11941 gccgcagtca ccgggctcgt cgtggacgtc ctgtctatgc gcaacccgtc tcgccgctgg
    12001 tatcttacaa cacaccaaat ttggctggac gctggtcgtt attgcaggtg gagccactaa
    12061 acgataccga aaggatgctg gcgctggcgg aaaatatgct cgaccgctac ggcatcatca
    12121 gtcgtcaggc ggtgatagcc gaaaatatcc ctggcgggtt tccatcgatg caaacgcttt
    12181 gtcgaagtat ggaagactcc gggcgaatta tgcgaggtcg ttttgtagaa ggtctgggtg
    12241 gcgcgcaatt cgctgaacgt ctgactattg accgattgcg cgatctggcg acacaagcca
    12301 cgcaaacgcg ccactataca ccagtggcgc tctctgccaa cgatccggct aatgtgtggg
    12361 gaaatcttct gccctggcct gcacatccgg caacgctggt tccaacgcgt cgggcgggtg
    12421 cgctggtggt cgtttctggc ggcaaattgt tactctatct ggcgcaaggt ggcaaaaaaa
    12481 tgctggtctg gcaggaaaaa gaggaattac tcgccccaga ggttttccac gcgctgacta
    12541 ccgcactgcg tcgcgaacca cggctgcgct ttacgctaac agaagtgaat gatctaccgg
    12601 tccggcaaac gccgatgttt acgctgctgc gtgaggcggg attttcaagt tcgccacaag
    12661 ggctggattg gggatagaga aaggactgac ggatgcccgt tcgcatccgt cagtattgca
    12721 ggacggatta ttccgcgtcc ggctcttcag acttgtattt agcggcagtt tctttgatca
    12781 gctgctgcag ttcgccacgc tgatacattt cgatcacgat atcacaaccg ccgaccagct
    12841 cgccgtcaac ccacagttgc gggaaggtcg gccagttagc atatttcggc agttccgcac
    12901 gaatgtccgg attctgcaga atatcaacat aggcaaaacg ttcgccacat gcggcaagcg
    12961 cctggactgc ctgggcagag aaaccgcagc tcggcagttt cggtgaacct ttcatgtaca
    13021 gcaggatcgg gttttcagca atctggcgtt ggattttttc gatagtggtg ctcattgtct
    13081 tgcttcctca aacttcttta cggcagtaat ctgacattgt agcgggtcag tgcggcatcg
    13141 gaaaataaca ttttcatcac gcttttgcta ttttatccct ttgctctatt tgttgcattt
    13201 caaatattcg tttcttctct gatgcgcggc aaactggcgt atttataacc atttttagct
    13261 ttcactgctg catttttttg cgctcgccaa cgaaacgtat tttttaacaa taaaagctat
    13321 taactttctc ttcttctatg cattagaatc atcaagtttt gtaaatcaga cgcaggcatg
    13381 atagacctgc cttaacagag ggacgctcag tggcgcggat aaaccgtatt tcgatcacgc
    13441 tctgtgcttt actttttacc accctgcctt taacgcctat ggcccatgct tcaaagcaag
    13501 ccagggagag ttctgctacc actcatatca ccaaaaaagc agataaaaag aaaagcacgg
    13561 caaccaccaa aaagacccag aaaacagcga aaaaagccgc cagtaaaagt acgaccaaaa
    13621 gcaaaaccgc ttcttccgtt aaaaaatctt ccattaccgc ttctaaaaac gccaaaactc
    13681 gcagcaaaca cgccgtcaat aaaacggcct cagccagctt taccgaaaag tgtaccaaac
    13741 gtaagggcta taaatcgcat tgtgtgaaag tcaaaaatgc cgcgtcagga actcttgccg
    13801 acgcgcacaa agcgaaggtg caaaaagcta cgaaagtggc aatgaataaa ctgatgcagc
    13861 aaattggtaa gccatatcgt tggggtggca gctcaccgcg taccggtttt gattgcagcg
    13921 gcctggttta ttacgcttat aaagatttgg tgaaaattcg tattccgcgt acggcgaatg
    13981 aaatgtatca cctgcgtgat gcagcgccaa tcgaacgtag tgaactgaaa aacggcgacc
    14041 tggtcttttt ccgtactcag ggacgcggca cagccgatca tgtcggcgtg tatgtcggca
    14101 acggcaaatt tattcagtca ccgcgcacag gtcaggaaat tcaaatcact tctctcagtg
    14161 aagactactg gcagcgccac tatgttggcg ctcgtcgggt aatgacccca aaaacacttc
    14221 gctaaaactt taccctgttg ttacggcaac agggtaagtt catcttttgt ctcacctttt
    14281 aatttgctac cctatccata cgcacaataa ggctattgta cgtatgcaaa ttaataataa
    14341 aggagagtag caatgtcatt cgaattacct gcactaccat atgctaaaga tgctctggca
    14401 ccgcacattt ctgcggaaac tatcgagtat cactacggca agcatcatca gacttatgtc
    14461 actaacctga acaacctgat taaaggtacc gcgtttgaag gtaaatcact ggaagagatt
    14521 attcgcagct ctgaaggtgg cgtattcaac aacgcagctc aggtctggaa ccatactttc
    14581 tactggaact gcctggcacc gaacgccggt ggcgaaccga ctggaaaagt cgctgaagct
    14641 atcgccgcat cttttggcag ctttgccgat ttcaaagcgc agtttactga tgcagcgatc
    14701 aaaaactttg gttctggctg gacctggctg gtgaaaaaca gcgatggcaa actggctatc
    14761 gtttcaacct ctaacgcggg tactccgctg accaccgatg cgactccgct gctgaccgtt
    14821 gatgtctggg aacacgctta ttacatcgac tatcgcaatg cacgtcctgg ctatctggag
    14881 cacttctggg cgctggtgaa ctgggaattc gtagcgaaaa atctcgctgc ataataactg
    14941 atggcaaatg cagcattgcc tgatgcactt cgcttaccat gccaacgcgg atgaggcgtt
    15001 aacagcgcat tcggcaagat aaatcgcacg ttgtcagcaa ctgtaacgca gaaggttatc
    15061 cttctgcgtt tttgtttaat tagctgttag caacgcaaac tgtttcaggt tgttttctgg
    15121 ctgacataaa caccagcaat aatgccagtc ccgcgacaat cgctcccatc accggcacaa
    15181 agctgtatcc cagcccagcg gaaattaccg caccaccagc agctgctccc agcgcatttc
    15241 caagattaaa ggcaccaata ttgactgatg aagacagacc tggcgcttca ctggcgacac
    15301 gcatcacgcg catctgtaac ggcggtacga ccgcaaaggt tgctgcgccc cacaccacca
    15361 tgctaatagc tgcgccgaac tcattgcggg ccaggaacgg gattgccagc ataatcacca
    15421 tcaacaacaa caaaaagcct ttcaacgtgc cgttaactga acgatctgcc agtttgccgc
    15481 cgagatagtt accgatagag aatccgacac caatcagcac cagcattgcc gtgacgaaca
    15541 ccggtgttgc gtgggtaata ctttgcagta ccggagagat ataggtgtag agagtaaaca
    15601 ttgcaccagc tcccagtacc gtcgtcagca atgcagacag cacctgcgga cgcattaata
    15661 ccgccagctc ttttttcact tcaggtcgtg cccctgcacc acctttaggt aatgagaaga
    15721 acagacttac cattgaaatc actcccagcc ccgccgttgc cagaaatgac atccgccagc
    15781 cgatggtttc acccaaccag gtcgccgccg gcacgccacc gatatttgcc agggttaacc
    15841 ccataaacat agtggcaact gcgctggcct gtttatgttt tggcaccacg cttgcggcca
    15901 cgactgaacc caaaccaaaa aatgctccgt gattcaggct ggtcaaaatg cgtgaaagca
    15961 tcagggtcat ataatccggc gcgatggcgg aaagtacgtt gccgagcgtg aaaattgcca
    16021 tcaggaaaat caacgcactg cggcgggcac gatgagaaag tagaagcgtc atcagcggcg
    16081 cgcccaccat tacgccaact gcataggcac tgattaacat tccggcagcg ggaatcgaga
    16141 catccacacc gcgcgcaatg acgggcaaca agcccattgg cgagaactcc gttgtcccga
    16201 taccaaacgc gccaatcgcc agcgccagca acggatagtt aattttcatg ccttatctcc
    16261 acctcttcgc gtcattacgc gatattcatt aaagtggcga aagcatgaca gcaatcacaa
    16321 aaaaatgaaa ataacaaaaa gagaaaacac ttttgccatt ttgctaacaa acaggaagga
    16381 gatgcgaggg agaacgcgct ccctcgagag gaaatcagtg cagcgcggca gtcaaaccca
    16441 cggctacgat caaaccgagg acgataatcg ttgttaccag tgaaaattta aggtcggtgc
    16501 tcatcaagtt ttctcctttt ttattaccac acaaaaagtg atattacgca tttttacaca
    16561 ctgtgatgaa aaaatctccc gtcatttata atgataagtg tttttaccac ttcccctttt
    16621 cgtcaagatc ggccaaaatt ccacgcttac actatttgcg tactggccat tgaccccttc
    16681 ctgacgctcc gtgtcgtttt tccggcgtac cgcaacactt ttgttgtgcg taaggtgtgt
    16741 aaaggcaaac gtttaccttg cgattttgca ggagctgaag ttagggtctg gagtgaaatg
    16801 gaatggcaac aataaaagat gtagcgaaac gagcaaacgt ttccactaca actgtgtcac
    16861 acgtaatcaa caaaacacgt ttcgtcgctg aagaaacgcg caacgccgtg tgggcagcga
    16921 ttaaagaatt acactactcc cctagcgcgg tggcgcgtag cctgaaggtt aaccacacca
    16981 agtctatcgg tttgctggcg accagcagcg aagcggccta ttttgccgag atcattgaag
    17041 cggttgaaaa aaattgcttc cagaaaggtt acaccctgat tctgggtaat gcgtggaaca
    17101 atcttgagaa acagcgggct tatctgtcga tgatggcgca aaaacgcgtc gatggtctgc
    17161 tggtgatgtg ttctgagtac ccagagccgt tgctggcgat gctggaagag tatcgccata
    17221 tcccaatggt ggtgatggac tggggtgaag caaaagctga cttcaccgat gcggtcattg
    17281 ataacgcgtt cgaaggcggc tacatggccg ggcgttatct gattgaacgc ggtcaccgcg
    17341 aaatcggcgt catccccggc ccgctggaac gtaacaccgg cgcaggccgc cttgccggtt
    17401 ttatgaaggc gatggaagaa gcgatgatca aggtgccgga aagctggatt gttcagggtg
    17461 actttgaacc cgaatctggt tatcgcgcca tgcagcaaat actgtcgcag ccgcatcgcc
    17521 ctactgccgt cttctgtggt ggcgatatca tggcaatggg cgcactttgt gctgctgatg
    17581 aaatgggtct gcgcgtcccg caggatgttt cgctgatcgg ttatgataac gtgcgcaacg
    17641 cccgctattt tacgccggcg ctgaccacga tccaccagcc aaaagattcg ctgggtgaaa
    17701 cagcgttcaa catgctgttg gatcgtatcg tcaacaaacg tgaagaaccg cagtccattg
    17761 aagtgcatcc gcgcttgatt gaacgccgct ccgtggctga cggcccgttc cgcgactatc
    17821 gtcgttaatc acccgttgcg ggagtctctt ccggctcccg cagccactcc ttattcagcg
    17881 tctcactatc gccgagatac tcaagcagcc aggttaacgc aggcgacata tcattttgct
    17941 gccatgtcag acaacatgcc gaatccggga aggggttttc caattcaagt gctacccact
    18001 tcccctcatt aagccacggt ttggcgaaat gtgttggcac catccctatg cataatcctg
    18061 ccgagataca ggttgccgat gattcccagt caggcacgac aactcttttt tggttatcca
    18121 gcaaccaggt aatacgttta ggtagcgttc gcgaggtgtc ttcgcgcacc aacgacggcc
    18181 agttgcgcaa cgtatcatcg ctgaatgggc catccatcaa cgccagcggg tggtggctgg
    18241 caacaacgca actccagctt agcatcccca tatcccggaa ggcataacga ccgcctaccg
    18301 gaatcgcgcg tgttgcgcca atcgccagtt ccacgcgccc gtcggaaagc gcatcccaga
    18361 caccgttgaa cacttcctga aagacaagaa gttcgacatc atcaaaatgg cgataaaaat
    18421 caacgatcat ctgccgtgta cgttctggcc tgacaatatt atccactgcg atagctaact
    18481 gaccgcgcca gccgttcgct atctgctgac attgctggcg ggtgatctgc atttttttga
    18541 caacagagcg cccttctttg agaaaccacg ctccagcagc ggtcagctcc acatcacggt
    18601 gccgtcgttc aaagagcggc accgccagcc actcttccag ctgacgcacg gtatagctga
    18661 ccgcagaagg aacgcgatgc agctcctgtg ccgcagcgct aaaactacca ttacgcgcta
    18721 ccgcatcaac aacttcgagt gaatattctg accacatagt ctacctgcaa aatttttgaa
    18781 accagtcatc aaatattacc gtttcacaac actaatttca ctccctacac tttgcggcgg
    18841 tgtttaattg agagatttag agaatataca tgcaacctgg gaaaagattt ttagtctggc
    18901 tggcgggttt gagcgtactc ggttttctgg caaccgatat gtatctgcct gctttcgccg
    18961 ccatacaggc cgacctgcaa acgcctgcgt ctgctgtcag tgccagcctt agtctgttcc
    19021 ttgccggttt tgctgcagcc cagcttctgt gggggccact ctccgaccgt tatggtcgta
    19081 aaccggtgtt attaatcggc ctgacaattt ttgcgttagg tagtctgggg atgctgtggg
    19141 tagaaaacgc cgctacgctg ctggtattgc gttttgtaca ggctgtgggt gtctgcgccg
    19201 cggcggttat ctggcaagcg ttagtgacgg attattatcc ttcacagaaa gttaaccgaa
    19261 tttttgcgac catcatgccg ctggtgggtc tatctccggc cctggctcct ctgttaggaa
    19321 gctggctgct ggtccatttt tcctggcagg cgattttcgc caccctgttt gccattaccg
    19381 tggtgctgat tctgcctatt ttctggctca aacccacgac gaaggcccgt aacaatagtc
    19441 aggatggtct gacctttacc gacctgctac gttctaaaac ctatcgcggc aacgtgctga
    19501 tatacgcggc ctgttcagcc agtttttttg catggctgac cggttcaccg ttcatcctta
    19561 gtgaaatggg ctacagcccg gcagttattg gtttaagtta tgtcccgcaa actatcgcgt
    19621 ttctgattgg tggttatggc tgtcgcgccg cgctgcagaa atggcaaggc aagcagttat
    19681 taccgtggtt gctggtgctg tttgcagtca gcgtcattgc gacctgggct gcaggcttca
    19741 ttagccatgt gtcgctggtc gaaatcctga tcccattctg tgtgatggcg attgccaatg
    19801 gcgcgatcta cccgattgtt gtcgcccagg cgctgcgtcc cttcccacac gcaactggtc
    19861 gcgccgcagc gttgcagaac actctacaac tgggtctgtg cttcctcgca agtctggtag
    19921 tttcctggct gatcagtatc agcacgccat tgctcaccac caccagcgtg atgttatcaa
    19981 cagtagtgct ggtcgctctg ggttacatga tgcaacgttg tgaagaagtt ggctgccaga
    20041 atcatggcaa tgccgaagtc gctcatagcg aatcacactg atctatatcg atatacttat
    20101 acttaggctg ctaacaaaat tttgttgtat gattgaaatt agcggcctat actaatttcg
    20161 agttgttaaa gctacgataa atattatgtt tttacgggga caggatcgtt cccgactcac
    20221 tatggatggt catttcggca aaggttcctc ctttccctct gttctacgtc ggattataga
    20281 ctcgcggttt tttctgcgag atttctcaca aagcccaaaa agcgtctacg ctgttttaag
    20341 gttctgatca ccgaccagtg atggagaaac tatgagttca tcgtgtatag aagaagtcag
    20401 tgtaccggat gacaactggt accgtatcgc caacgaatta cttagccgtg ccggtatagc
    20461 cattaacggt tctgccccgg cggatattcg tgtgaaaaac cccgattttt ttaaacgcgt
    20521 tctgcaagaa ggctctttgg ggttaggcga aagttatatg gatggctggt gggaatgtga
    20581 ccgactggat atgtttttta gcaaagtctt acgcgcaggt ctcgagaacc aactccccca
    20641 tcatttcaaa gacacgctgc gtattgccag cgctcgtctc ttcaatctgc agagtaaaaa
    20701 acgtgcctgg atagtcggca aggagcatta cgatttgggt aatgacttgt tcagccgcat
    20761 gcttgatccc ttcatgcaat attcctgcgc ttactggaaa gatgccgata atctggaatc
    20821 tgcccagcag gcgaagctca aaatgatttg tgaaaaattg cagttaaaac cagggatgcg
    20881 cgtactggat attggctgcg gctggggcgg actggcgcac tacatggcat ctaactatga
    20941 cgtaagcgtg gtcggcgtca ccatttctgc cgaacagcaa aaaatggctc aggaacgctg
    21001 tgaaggcctg gatgtcacca ttttgctgca agattatcgt gacctgaacg accagtttga
    21061 tcgtattgtt tctgtgggga tgttcgagca cgtcggaccg aaaaattacg atacctattt
    21121 tgcggtggtg gatcgtaatt tgaaaccgga aggtatattc ctgctccata ctatcggttc
    21181 gaaaaaaacc gatctgaatg ttgatccctg gattaataaa tatatttttc cgaacggttg
    21241 cctgccctct gtacgccaga ttgctcaatc cagcgaaccc cacttcgtga tggaagactg
    21301 gcataacttc ggtgctgatt acgatactac gttgatggcg tggtatgaac gattcctcgc
    21361 cgcatggcca gaaattgcgg ataactacag tgaacgcttt aaacgaatgt ttacctatta
    21421 tctgaatgcc tgtgcaggtg ctttccgcgc ccgtgatatt cagctctggc aggtcgtgtt
    21481 ctcacgcggt gttgaaaacg gccttcgagt cgctcgctaa aggctattct atcgccccct
    21541 ctccgggggc gatttcagat caggcttctg tacctggttg attcatggca ttttctcgtg
    21601 ccgccagcac acgttctacc gtatctacca ctgcctgagt ttgtggatca atttcaatgt
    21661 tgacgcgtgc gccgagtttt ttcttcccaa gagtcgtgcg ttccagagtt tccggaatta
    21721 aatggacgca aaaacgcgtt ggcgtgactt cgccgacggt caggctaata ccgtcgatgc
    21781 caataaatcc tttgtacagg atatatttca tcaactgact atcctggact ttaaaccaga
    21841 tctggcgatt attttccgag gttaatattt tcgccacttc agcggtggtc ataatatgac
    21901 ctgacattaa gtgtccgcca atttcatcac tgaatttcgc cgcacgctca acgtttaccc
    21961 aatcccccac ttttaaatcg ccaagattgg taatgcgtaa cgtttctttc atcaggtcaa
    22021 aactgacatg gttgccgtta atttccgtca cggtcaggca gcaaccgtta tgcgccacgg
    22081 aagcacctgt ttccaggccg tccagcatgt ggtcgggtaa ctccaccaca tgcgtacgaa
    22141 aatttggttt ctcgtcaatc gacaccagtt ttgcggtgcc ctgtacaatc cccgtaaaca
    22201 tacttacaac tcctgaaatc agttaagaca ttctgttcag cacaatagca ggtggcaaac
    22261 gcccttacca gtgaaggggt aagaatgact attttttcac tggagaatta ataaatcctc
    22321 gctacaatag actgaatttc ccctgcttct tctttttgct gcccattcag gcggcttttt
    22381 agtctctcat ataactacaa ataaaaggtg ttcacgtgca gaagtatatc agtgaagcgc
    22441 gtctgttatt agcattagca atcccggtga ttctcgcgca aatcgcccaa actgcgatgg
    22501 gttttgtcga taccgtgatg gcgggcggct atagtgccac cgacatggcg gcggtcgcta
    22561 tcggtacttc tatctggctt ccggcgatcc tctttggtca cggactgctg ctggcattaa
    22621 cgccggttat cgcgcaatta aatggttccg gtcgacgtga gcgcattgcg catcaggtgc
    22681 gacaaggttt ctggctggca ggttttgttt ccgttctcat tatgcttgtg ctgtggaatg
    22741 caggttacat tatccgctcc atggaaaaca tcgatccggc tctggcggac aaagccgtgg
    22801 gttatctgcg tgcgttgttg tggggcgcgc cgggctatct gttcttccag gttgcccgta
    22861 accaatgtga aggtctggca aaaaccaagc cgggtatggt aatgggcttt atcggcctgc
    22921 tggtgaacat tccggtgaac tatatcttta tttatggtca tttcggtatg cctgagctcg
    22981 gtggcgttgg ttgtggcgtg gctactgcgg cggtgtattg ggtcatgttc cttgccatgg
    23041 tttcttacat taaacgcgcc cgctccatgc gcgatattcg taacgaaaaa ggcaccgcga
    23101 aacccgatcc tgcggttatg aaacgactga ttcaactcgg tttgccgatt gcgctggcac
    23161 tattctttga agtgacactg tttgccgtcg tggctctgtt agtgtctccg ctcggtattg
    23221 ttgatgtcgc aggacaccag attgccctga actttagttc actgatgttt gtgcttccaa
    23281 tgtcgctggc ggcagcggta actatccgcg taggttatcg tctgggtcag ggctcaacgc
    23341 tggatgcgca aaccgctgcg cggaccgggc ttatggtggg tgtctgtatg gcaaccctga
    23401 cggccatatt cacggtttca ctgcgggagc aaatcgccct gttgtacaac gacaatcccg
    23461 aggttgtaac gctggctgcg catttgatgt tgctggcggc ggtatatcag atttctgact
    23521 caatccaggt gattggcagt gggattttgc gtggttataa agatacgcgt tccattttct
    23581 atatcacctt tacggcttac tgggtgctgg gcttgccaag cggctatatt ctggcactga
    23641 ccgatctggt cgttgaacct atggggccag caggcttctg gataggcttt attattggcc
    23701 tgacgtcggc agccattatg atgatgctgc gtatgcggtt cctgcaacgt ctgccgtcag
    23761 ttatcattct gcaacgagca tcccgctaat aaagacaagg cgcaaccttc acgggttgcg
    23821 cctgtgtttt tacgcaggct ggagcgttgc gccaatcccg tcttcgtctg gctgtaattt
    23881 cagagcgttg cacagagttt gccgactggc atctgttatc tcaacgtgta tatccccggt
    23941 aacttcccct ttcacattga tgaagtcagg tgagatttct ttcgcactgg tttgcaggca
    24001 aatcgctccc tctccgcgca ggcgtttcac tttcagttgc acatgcgttg ccgcaccagt
    24061 gatcagccac agttttccgc cattcagatt gatgttacca acaataaaag tgacaccgtc
    24121 accttttacc gtactgccgt cgagaatcaa cacaccgccc tgctgtacag taatcgttga
    24181 ttgttgcaga tctgcgccat agccaataat cattgaagcg ccgtccagta atgcaacact
    24241 cccggtcagt tcaaccggtg cgcgatcttc ctctacgaca aaattttcgt ccgcgctggt
    24301 gggtgacaac aggatcactg ttccaccttg tattaccgca ttttccagcg taccggcatg
    24361 cacttcagcc cgaccaccca cattcacgga caggttttgc gtcttaccgg aactgtagag
    24421 ctgaaggcca tccgtcccca gacgataaat ggatccattc tcaacaatag tgtcagagtc
    24481 ttcaccaccg agattattca tggtgccacc agcaagcaac gtatcactgg ctttcccggc
    24541 cagcataaaa acgccgccct gattaatctc acaattgctg gcttccccac ctggatacac
    24601 caacaattca ccaccttcat ctaccacgac cgctgaggtt gtcccattaa ccaacaggcc
    24661 gccctcttga tcgagaatga ttttttccgc gcgatgtcct tccagtacac gcaggttacc
    24721 gccattttcc agcaacaaac cgcaggcata accctgatca acgctgaatt caccttcggg
    24781 atggcggcca ttaaccgtag cgagcgtaga gagactaatt gcacctccct cggactgaac
    24841 aatatctgtg gcaatggcac cgtcgctgac cgatagcgtg ccattgttac cgtactgtgt
    24901 gcatttggcg atcccaccgg ttgccacttt caaccagcca gcccccgtga ccacggtatt
    24961 ttcggctctt ccgccagctt caacaatcaa ctcgccttca gtaagttgcg tcgcgccaat
    25021 gacctcgccg gtcttaacaa tttgcacatt cccgtcgctc atcaagtttt tcgcatcaga
    25081 tcccatgatt tattcctttg ctgcatctgt gtgcctttat tgctacctaa gtgtaaaggc
    25141 tacggaggat ttatccacga cagatttgag atggtggcaa acaactctgt ttaaactctg
    25201 atacacgaat tattgggttg tatcagatgt aaatgcgatc ctgaataaaa atcgcccttg
    25261 caaatcaaca aaatatagcc aatttgctta aaagctctgc aaacggtcga aatagcgcag
    25321 aaaattacgt tttgcctctt gccaccttcc cactctgccg ctaatattcg tccccgttgt
    25381 cacctacaac gttgcgttca tagctcagtt ggttagagca ccaccttgac atggtggggg
    25441 tcgttggttc gagtccaatt gaacgcacca tcctgcgtcc gtagctcagt tggttagagc
    25501 accaccttga catggtgggg gtcggtggtt cgagtccact cggacgcacc agattttctt
    25561 aatctggtct tctccttttt ccctctgttt cttctctgta tccaatacgt taaaagattt
    25621 acactgtctt cgtatgcgtt atcagaagga gaatcgctat ggcaactttg ttacaacttc
    25681 attttgcttt taatggccca tttggcgacg caatggctga gcagcttaaa ccgcttgctg
    25741 agtcgattaa tcaggaacct ggttttctgt ggaaggtatg gacagaaagt gaaaagaacc
    25801 acgaagccgg tgggatctat ctttttactg atgaaaaaag cgcccttgcc tatctggaaa
    25861 aacatactgc ccgacttaaa aatctcggcg ttgaggaagt tgtcgccaaa gttttcgatg
    25921 tcaatgagcc actgagtcaa atcaatcagg caaaactcgc ctgacagaat ttaatcaagg
    25981 gcggttagcg cccttttcat ccctgtctga aatttctcaa attctaaaaa tctcaaccaa
    26041 acttatctga taacactaaa ttcgaaagaa tgcgtacagg taagtaacaa tgaaaaaaat
    26101 tgctattgtg ggtgccgggc ctacggggat ctacacctta ttctcgcttc tacagcaaca
    26161 aactccactt tctatttcta tcttcgagca ggctgacgag gccggtgtcg ggatgccata
    26221 cagtgatgag gaaaactcaa aaatgatgct ggcaaatatt gccagtattg aaataccgcc
    26281 gattaattgt acgtatctcg aatggctaca aaagcaagaa gccagccatc tccagcgtta
    26341 tggcgttaaa aaagaaacct tgcacgatcg tcagttttta ccgcgaattc tgctgggcga
    26401 atatttccgc gatcaatttt tacgattagt agaccaggca cgacagcaaa aatttgcagt
    26461 ggctgtttat gaatcatgcc aggttaccga tctgcaaatt acaaatgctg gcgtcatgct
    26521 cgctacaaat caggatttac ccagcgagac gtttgattta gcggtgattg ccacgggtca
    26581 cgtctggcct gatgaagaag aagcaaccag aacgtatttt cccagtccgt ggtcaggcct
    26641 gatggaagca aaggtcgatg cgtgtaacgt gggtattatg ggaacatcct tgagcggact
    26701 ggatgcggca atggcagtgg ctattcagca tggttcgttc attgaagatg ataaacaaca
    26761 cgtcgttttt aaccgcgata acgcaagtga aaagctaaat attacgttga tgtcgcgcac
    26821 gggtatttta cccgaagccg atttctattg ccctattccc tacgagccct tacacatcgt
    26881 caccgatcag gcattaaatg ctgagattca aaaaggtgaa gagggccttt tggatcgggt
    26941 atttagattg atagtagagg aaatcaagtt tgctgatcca gactggagcc aacgcatagc
    27001 cttagagagc ctgaatgtcg attcctttgc tcaagcctgg tttgccgagc gcaaacaacg
    27061 cgacccattt gactgggcag aaaaaaatct ccaggaagtc gaacgcaata aacgagaaaa
    27121 acatactgtt ccctggcgtt atgtcattct gcgcctgcat gaagccgtac aggaaattgt
    27181 tcctcatctg aatgaacacg accataaacg gttcagtaaa ggccttgcca gggttttcat
    27241 cgataattat gcggcaatcc cttcagagtc tattcgtcgc ctacttgcct tacgtgaagc
    27301 gggaatcatt catattctcg ccctcggtga agactacaaa atggaaatta atgagtcgcg
    27361 caccgtcctg aaaacggaag acaacagcta ctcgtttgac gtttttattg atgcccgcgg
    27421 acagcgtccg cttaaagtga aagatattcc tttccctggg ctacgcgagc aattacagaa
    27481 aacaggggat gaaatccctg atgttggcga agattatacg ttacagcaac ccgaagatat
    27541 tcgtggacgc gtagcgttcg gcgcgttgcc ctggttgatg cacgaccagc ctttcgttca
    27601 gggacttacg gcatgtgcag aaattggtga ggcgatggct cgggcggtcg taaagcctgc
    27661 atcccgtgct cgtcggcgtc tttcgtttga ttaaaggtaa agctatcagc actcaacaaa
    27721 ctgatgctgt tcattaagac gatagcggac tccggttcga atattgtttt caccttcaat
    27781 ggcgacggcg aagcgaactc tttcgccgtc atgatatgcc agtgcggcgc ttccccctgg
    27841 cccgagaata attgagtcga ccacgcccgt gctcactacc gttgaatttt caccgctggc
    27901 aatgatgtgt acattgtcgc cgctgttggc aattctggcg tttgcgccaa agctggcgat
    27961 ttgcaccagg tcaccattac tggcgacatg acaccgttca cccaatgtac aaacgcgtac
    28021 tcgcattccg gtattcgcaa tccgactact atctccagcg ctgcttatcc gcgaagaatt
    28081 accggttaca gcaaccagtg cgcgttcacc ggagctgcca atgtgactgt taaagccgac
    28141 acttccaatc cgcacgctat aacccatact ggcaatttgc gcggcatacc cagcggttgc
    28201 gattcgggcg ttatcctcac aacagccgat gcgagcatga tcgtcgctgc gggcaaactg
    28261 actgcaaaat ggtatctctc cggggtctgt ggccagtttt tgcatagagg aaacttcctg
    28321 atgcgcgaag ttttcatccg ccagccattt tttccacgca tactcaacca gactctccgc
    28381 ccagtccgta tatccttcct caatcagtgc atgatgaata tcggcataac taccgccgcg
    28441 tgggaaatgg cgcaggaacc agcgatacat caccgctccg atacgccatt ctcgcaggtc
    28501 tgctcgtgta atgatcattc gttagtgccg gtgatagcca tcgaccatgc ttttaaaggt
    28561 ttcgtgtggc gtacgccccg tggtgcaaag ataaagatga ccgaagataa aaaagaggct
    28621 tataaatgcc agagcaaaat gcgcctgcaa taaccagtat cttacgccag gaaacacatc
    28681 tcccacggct tgcggataga gacacaacag ccccgtcaat agtaacaacg gcagcaatcc
    28741 atacatgaca ccaacatagg cgacctgctg taagggatta aatttagact gtgttgttgc
    28801 cgggaaagga tgttcttccc cctgcataat gccaaacaaa taaaatcgcg tttgttttgc
    28861 cgctcgttcc agccacccct gacgacgaat gcgatagtgg tgaccattat ccccaacggc
    28921 attgatcagc acaaagccga gccagcatgc cagtaacaaa aatccgcaaa cttcatgcac
    28981 cgcaaccaga cttttaaccg cagttgcgcc caccatcgca aagtgattta tcagcccact
    29041 tgccagcaac aatacaaaga gtaacgcatt cgaccagtgc cataatctga ccgctttgct
    29101 gtaaagataa accttctcac catgatcggt ggctgatttc ttcacgccac gagcacgaag
    29161 caaggcgtgc agccccagca ccaaccacat cccaaccagc aatactccgg caatgatcaa
    29221 ccacaccggc caaaattcgg gagtgaactg tggcacatag ttcgctaact ggctctgaaa
    29281 ctgttcagca tgttgcgacg agttcataca ttttcctttt taatcaaatg ttgaccgaac
    29341 cggcgataca gatgcggttt cccggcacca ggtagctgat attgatagta tttattatcc
    29401 tgcaaccacg cctgaatttc tgggctatct tcgcgaccaa agatcaatgc gtgttccgga
    29461 caggcgctga cgcaaatggg cggaaagcct tttgccagtc gggactcagc gcagaaatca
    29521 catttatctg ccactttagt gacagggtta aggtagcgaa cctggtacgg acaagcgcca
    29581 atgcaataac tgcagccgat gcactgcgat ttttctaccc gcacgatccc ctgctcatca
    29641 cgccacgacg cccctgtcgg gcatacgtcg atgcacggag catcttcgca gtgctgacat
    29701 gactggcgaa aaaagtgata ctgcgtttcg ttgtcgttat cggtgactgg aatgtgtgct
    29761 atcgataaac ggcttccctg agcggggacg tggttagttt tacggcatgc gcgggcgcaa
    29821 atattacagc cattgcaccg tgactcgtca tgaatcatgg cataccgaac ttccttttct
    29881 tgcctcgtgt tcgctaacag agaagacgca gaaccagtaa aaaagattac tgttcccatc
    29941 cccagaacaa atttgcgtcg agtgaaggac atctgattat cccctaatgc agtggctgca
    30001 actgtccacc ctatccatga catctccctg aaatttcagg ggttctgaaa ctgctctacc
    30061 tcgaaccatt cgcataagcc ataagccttg ccataggtca taaatcgctc gctaaacaag
    30121 tacaacaccg ccgtcgatga ctccacctgc cgtatatcgg catattcagg tgcaacatcc
    30181 atcgcggcga tcgcggcttc aatctgcgct tcctggaagt aatatggcgc ttgcatcagc
    30241 atggcgactt tgtaaggacg aggatatgtc tggcactcaa aacgaacggc gtgtgcaatg
    30301 gcgcgacaaa tgtcctgctc cacaacttgc aaagacatcg ccgcgtagtt ttcactcatg
    30361 gcctgcgttg agtaatagta atcatcctgg cttcccgtca tacaagcgat atccgcgtag
    30421 tctccgttct gcttaatgct ttccagcaaa actgacagct cctcttctgc aaccgaatag
    30481 ggtggcgaga gaaacacggc gcgcgccacc agctgcccag ccgcagaatg ctggcgaatg
    30541 aactctgcca ggatttcagc cggttctggt tcggttaccg tatcaacggg gtgacgcatc
    30601 ccctgtaacc attgccgctt cgcctcgtca acttcgctga ctttcgctaa cggcaattca
    30661 tcccgatggt tcatctcacc cattccttac acaggcagta gattgtgtgc tgccagatcg
    30721 gcggcaatat cttccagccc cagacgctgc aatgtttcgc gggtcggaca accaagctgt
    30781 gggtcccagc ccatttcttt gtagaacatc gtgagcgaag catgcatatc gtcacgatcc
    30841 attttgtcag taccttcagt aaagaccggg atctgcggat ccttgtcgaa tacccaggaa
    30901 cagataagat cgtgttcatt acgcatatct ttggtttgca tcagttttac cgtgtaggca
    30961 cgatgcaacg taaagatacg ctctgcagct aaatctaatt tttcctgagt catctcttcg
    31021 ccggtgatcg ctttgaagaa tttggcttca agcgccagat cgccccggta attacggctt
    31081 ttcagcggcg aaacggtcat tggccagacc cagttgcaca gggtgacggc gttatgcaaa
    31141 cagacccgca acagcgacca ctttgcatat ttaatttttg cgtcattgat tggcgtgtag
    31201 tttttggttt catcgtaggc atcttcagaa ccaaaaagct ctttcgccac ttcacgctgc
    31261 agtttcaatg gcaaacccga accaataaaa ttgatatggg tatgcgtcat gcagtcacgg
    31321 ttgaacatac agttaacaat ggaaccgacc tgcgctgacg cttcattggc atggtgaacc
    31381 ggatagccaa acggcgacca gagtttattt ttcgcgtagc cccagtactc ttcacccaaa
    31441 ttccagcgtt ctgcgatggc atatgaacca tcagccaggt gactcagctc acccacacga
    31501 tgcgccagac ggtagtaaaa atctttaatg aagttaacgt cacccgcttc cagttgatcc
    31561 cagcgaattt ccgcatactc ttcagcaggc agaacacgct tgaacacccc tttgctgtaa
    31621 caataggtaa agtcgcgatg caactgcccg tagttacacc ataggccgta gtcatcgaac
    31681 aaattcagac ccaccaggtt accaatcaca cggccatcgt ctttatcttc aaaatctttc
    31741 ggaccgttcg ggaagatggt ggtatggaca aagttggcta cacaagtgtt accacctgtg
    31801 ctgggcacgc caaactcttt cacccgagga atattcattt gggtcataca acggatcggg
    31861 caagagtggc agccactcat ttttactgtg tatttctctg ccgccggacc taagtcaaaa
    31921 acggatttat aggtacgaaa gccgaccgtg ttctgattgc ctggcggaat ttcccccgtt
    31981 tcaatcggac caccttcagc cgcgccccaa aacagccctt tacgtgctgt ccaacgtgac
    32041 ttgggatctg aatactctgc ccacgattgc ggcgtacttg gcacgacatg gttgttattc
    32101 gcaccaataa gttcagtcat catgtaatcg ttgagacgct tcatctcctg acgatcggca
    32161 atgttgaccc ctttcgtccc ttcaaccgca atcgctttca ggtttttcga acccataatt
    32221 gcgccagttc ccgcaccgcc gctgtggtta cggctattca acatgccaga gagaggaaca
    32281 aggttttccc cagcctgacc aatagccgcc acacaggttt ccggactggt caatcgacaa
    32341 atttcttccg tcgttgcgcg cgtccctttt ccccataaga aatcggcttt ttccaggcta
    32401 actttgtcat ctttaatctt cagccatacc ggtgatttcg ccttcccttc gataataatg
    32461 acgtcgtatc cagcgaattt catttgcgct gcaaaaaagc cacccatatg ggcatcgaca
    32521 actaaatttc ctttggtaaa agtagaaagt gaggtgatat ttacgcgaga actacagggg
    32581 gcaccagatc cagttaatgg gccggtagca aagactaatt tattcgcttc atcgaaaggt
    32641 ttcgtgcctg gcggtacttc gtcatacata attttgtagc cgaagcccat gccaccgaca
    32701 aaacttttaa acttactgga atcttcgagg gtaatatttc ctgtcgtgag attgactctt
    32761 aatatattac ctgtccaacc gttagccatg attttttcct ttgcaagatt cacacagtaa
    32821 tatctttcca ctcgataatt tttaacgccc ctgttgggca ggcgtttgcg cattcaccgc
    32881 ataacacaca tttcgaggat tttttacttt cggtatttac ggtggccatc atccacgggc
    32941 atgccgtagt acaggcgcta cagccaatac agcgtttatg atcgacggta atacagcctt
    33001 ctttctgctg ccaggtaatc gcaccaatcg ggcagacgtt catgcattgc ggctctttgc
    33061 attgacggca ggtgtccgcg gtatagttga gatcgccata caggccgccg ccagagccaa
    33121 ccccgttgtc gccaaagaaa taattgcggt ggattttgat acgggagaag aatgtcccta
    33181 ctgagccatc gttgaagttg gtacaagaga tttcacagcg gtggcaaccc gtgcatcgcg
    33241 ctcgctgggt caccagcacc cctttgggtg tatttatcaa ccctactgtg ccgctatcaa
    33301 tatcttcctg tttgcaaccc aaaagtgaca gtaacgcagg ggcaattgtt agaccagcaa
    33361 ggccttttcc tgatatacgc aagaattcta atcgcgttaa gccaatatct aatagtggac
    33421 gatcaaccgg gttcatttat ttcgcatcct cttcgcaaac acagtgctga caattcagca
    33481 gcctgatatt aagcattcaa ctaatacgct gctgactctg tccccaacac cagatatttt
    33541 ccctaacccg atagtgtatg agaaaagatt taacgccgat aaactaatcc tttatggtta
    33601 ggagaaaaaa taatgtgatt attctgagcc cttaa
//
LOCUS       DAPHDW010000037        32969 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.037, whole genome shotgun sequence.
ACCESSION   DAPHDW010000037 DAPHDW010000000
VERSION     DAPHDW010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.037"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            30..935
                     /locus_tag="RH057_001762"
     CDS             30..935
                     /locus_tag="RH057_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005100755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3128143.1"
                     /translation="MLIVSPSVVASEKAHELYDSIYGGKPAPDVINTLHKMAESGDID
                     AQSLLGWEYYQPRYDTKPDVQEAIKWFELAAKQGDREAPLALGGIYYDGEQVRVDYAK
                     AYALFNQAAQHGGNLAWSRLGIMYANGQYVEVDCKKAKEYLDKGVHIYGGPEDFLATC
                     RKDMIDRKTVDDTLPVITVTRSGMRDNFLDKGFSCMDSLFATTNKLGEVANLRVTFSI
                     RRPSGKEINQTVGFAPFGLNRLNISFTDYLFGSFTSNSSLILYKPEFERKSCATVRTT
                     IVAATATINGKDVELLKAGAIEQKW"
     gene            complement(991..1533)
                     /locus_tag="RH057_001763"
     CDS             complement(991..1533)
                     /locus_tag="RH057_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3128144.1"
                     /translation="MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRAS
                     LKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEKQRVSGVLQGSQPSEIGVVRLVM
                     LGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
                     TPNAFGAVPMFLAIQHILAA"
     gene            complement(1517..1828)
                     /locus_tag="RH057_001764"
     CDS             complement(1517..1828)
                     /locus_tag="RH057_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HDV3128145.1"
                     /translation="MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            complement(2013..2903)
                     /gene="cyoE"
                     /locus_tag="RH057_001765"
     CDS             complement(2013..2903)
                     /gene="cyoE"
                     /locus_tag="RH057_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HDV3128146.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPPVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            complement(2915..3244)
                     /locus_tag="RH057_001766"
     CDS             complement(2915..3244)
                     /locus_tag="RH057_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HDV3128147.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            complement(3244..3858)
                     /locus_tag="RH057_001767"
     CDS             complement(3244..3858)
                     /locus_tag="RH057_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HDV3128148.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            complement(3848..5839)
                     /gene="cyoB"
                     /locus_tag="RH057_001768"
     CDS             complement(3848..5839)
                     /gene="cyoB"
                     /locus_tag="RH057_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
                     /protein_id="HDV3128149.1"
                     /translation="MFGKLSLDAVPFHEPIVMVTIAGIILGGLALVGLITYFGKWTYL
                     WKEWLTSVDHKRLGIMYIIVAIVMLLRGFADAIMMRSQQALASAGEAGFLPPHHYDQI
                     FTAHGVIMIFFVAMPFVIGLMNLVVPLQIGARDVAFPFLNNLSFWFTVVGVILVNVSL
                     GVGEFAQTGWLAYPPLSGIEYSPGVGVDYWIWSLQLSGIGTTLTGINFFVTILKMRAP
                     GMTMFKMPVFTWASLCANVLIIASFPILTVTVALLTLDRYLGTHFFTNDMGGNMMMYI
                     NLIWAWGHPEVYILILPVFGVFSEIAATFSRKRLFGYTSLVWATVCITVLSFIVWLHH
                     FFTMGAGANVNAFFGITTMIIAIPTGVKIFNWLFTMYQGRIVFHSAMLWTIGFIVTFS
                     VGGMTGVLLAVPGADFVLHNSLFLIAHFHNVIIGGVVFGCFAGMTYWWPKAFGFKLNE
                     TWGKRAFWFWIIGFFVAFMPLYALGFMGMTRRLSQQIDPQFHTMLMIAASGAVLIALG
                     ILCLVIQMYVSIRDRDQNRDLTGDPWGGRTLEWATSSPPPFYNFAVVPHVHERDAFWE
                     MKEKGEAYKKPDHYEEIHMPKNSGAGIVIAAFSTIFGFAMIWHIWWLAIVGFAGMIIT
                     WIVKSFDEDVDYYVPVAEIEKLENQHFDEITKAGLKNGN"
     gene            complement(5861..6808)
                     /gene="cyoA"
                     /locus_tag="RH057_001769"
     CDS             complement(5861..6808)
                     /gene="cyoA"
                     /locus_tag="RH057_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit II"
                     /protein_id="HDV3128150.1"
                     /translation="MRLRKYNKSLGWLSLFAGTVLLSGCNSALLDPKGQIGLEQRSLI
                     LTAFGLMLIVVIPAILMAVGFAWKYRASNKDAKYSPNWSHSNKVEAVVWTVPILIIIF
                     LAVLTWKTTHALEPSKPLAHDEKPITIEVVSMDWKWFFIYPEQGIATVNEIAFPANTP
                     VYFKVTSNSVMNSFFIPRLGSQIYAMAGMQTRLHLIANEPGTYDGISASYSGPGFSGM
                     KFKAIATPDRAEFDQWVAKAKQSPNTMSDMAAFEKLAAPSEYNQVEYFSNVKPDLFAD
                     VINKFMAHGKSMDMTQPEGEHSAHEGMEGMDMSHAESAH"
     gene            complement(7268..8743)
                     /gene="ampG"
                     /locus_tag="RH057_001770"
     CDS             complement(7268..8743)
                     /gene="ampG"
                     /locus_tag="RH057_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HDV3128151.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
     gene            complement(8787..9365)
                     /locus_tag="RH057_001771"
     CDS             complement(8787..9365)
                     /locus_tag="RH057_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3128152.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRDLRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            9670..9987
                     /gene="bolA"
                     /locus_tag="RH057_001772"
     CDS             9670..9987
                     /gene="bolA"
                     /locus_tag="RH057_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HDV3128153.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            10331..11629
                     /gene="tig"
                     /locus_tag="RH057_001773"
     CDS             10331..11629
                     /gene="tig"
                     /locus_tag="RH057_001773"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HDV3128154.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            11875..12498
                     /gene="clpP"
                     /locus_tag="RH057_001774"
     CDS             11875..12498
                     /gene="clpP"
                     /locus_tag="RH057_001774"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HDV3128155.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            12624..13898
                     /gene="clpX"
                     /locus_tag="RH057_001775"
     CDS             12624..13898
                     /gene="clpX"
                     /locus_tag="RH057_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HDV3128156.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            14086..16440
                     /gene="lon"
                     /locus_tag="RH057_001776"
     CDS             14086..16440
                     /gene="lon"
                     /locus_tag="RH057_001776"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HDV3128157.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDAPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVTGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            16649..16921
                     /gene="hupB"
                     /locus_tag="RH057_001777"
     CDS             16649..16921
                     /gene="hupB"
                     /locus_tag="RH057_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HDV3128158.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            17113..18984
                     /gene="ppiD"
                     /locus_tag="RH057_001778"
     CDS             17113..18984
                     /gene="ppiD"
                     /locus_tag="RH057_001778"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV3128159.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLDAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGAPG
                     INSDIITVDGDRAFVLRISEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKILSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            19135..19506
                     /locus_tag="RH057_001779"
     CDS             19135..19506
                     /locus_tag="RH057_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HDV3128160.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLLERNLAVLTL"
     gene            19600..19998
                     /gene="fadM"
                     /locus_tag="RH057_001780"
     CDS             19600..19998
                     /gene="fadM"
                     /locus_tag="RH057_001780"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HDV3128161.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGLENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILSQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(20050..20745)
                     /gene="queC"
                     /locus_tag="RH057_001781"
     CDS             complement(20050..20745)
                     /gene="queC"
                     /locus_tag="RH057_001781"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HDV3128162.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(20810..22510)
                     /locus_tag="RH057_001782"
     CDS             complement(20810..22510)
                     /locus_tag="RH057_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
                     /protein_id="HDV3128163.1"
                     /translation="MRLLNRLNQYQRLWQPSAGKPQAVTVNELAERCFCSERHVRTLL
                     RQAQEAGWLEWQAQSGRGKRGQLRFLVTPESLRNAMMEQALETGKQQDVLELAQLAPG
                     ELRTLLQPFMGGQWQNDTPTLRIPYYRPLEPLQPGFLPGRAEQHLAGQIFSGLTRFDN
                     NTQRPIGDLAHHWETSTDGLRWDFYLRSTLHWHNGDAVKASHLHQRLLMLLQLPALDQ
                     LFISVKRIEVTHPQCLTFFLHRPDYWLAHRLASYCSHLAHPQFPLIGTGPFRLTQFTA
                     ELVRLESHDYYHLRHPLLKAVEYWITPPLFEKDLGTSCRHPVQITIGKPEELQRVSQV
                     SSGISLGFCYLTLRKSPRLSLWQARKVISIIHQSGLLQTLEVGENLITASHALLPGWT
                     IPQWQVPDEVKLPKTLTLVYHLPIELHTMAERLQATLAAEGCELTIIFHNAKNWDDTT
                     LLAHADLMMGDRLIGEAPEYTLEQWLRCDPLWPHVFDAPAYAHLQSTLDAVQVMPDEE
                     NRFNALKAVFSQLMADATLTPLFNYHYRISAPPGVNGVRLTPRGWFEFTEAWLPAPSQ
                     "
     gene            22610..23428
                     /gene="cof"
                     /locus_tag="RH057_001783"
     CDS             22610..23428
                     /gene="cof"
                     /locus_tag="RH057_001783"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HDV3128164.1"
                     /translation="MARLAAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHCDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKEIPALLQAFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVLTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            23581..24039
                     /gene="decR"
                     /locus_tag="RH057_001784"
     CDS             23581..24039
                     /gene="decR"
                     /locus_tag="RH057_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HDV3128165.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGFWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            24069..25841
                     /locus_tag="RH057_001785"
     CDS             24069..25841
                     /locus_tag="RH057_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV3128166.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGQILMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVAQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDAPEIREEAVDA"
     gene            25834..27615
                     /locus_tag="RH057_001786"
     CDS             25834..27615
                     /locus_tag="RH057_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HDV3128167.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKMVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGVVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDER
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIRLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDGIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIVDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            27796..28134
                     /gene="glnK"
                     /locus_tag="RH057_001787"
     CDS             27796..28134
                     /gene="glnK"
                     /locus_tag="RH057_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HDV3128168.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            28164..29450
                     /gene="amtB"
                     /locus_tag="RH057_001788"
     CDS             28164..29450
                     /gene="amtB"
                     /locus_tag="RH057_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HDV3128169.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSAGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(29498..30358)
                     /gene="tesB"
                     /locus_tag="RH057_001789"
     CDS             complement(29498..30358)
                     /gene="tesB"
                     /locus_tag="RH057_001789"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HDV3128170.1"
                     /translation="MSQALKNLLTLLNLEKIEEGLFRGQSEDLGLRQVFGGQVVGQAL
                     YAAKETVPEERLVHSFHSYFLRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIF
                     YMTASFQAPEAGFEHQKTMPSAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEV
                     RPVEFHNPLKGHVAEPHRQVWIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHGIG
                     FLEPGIQIATIDHSMWFHRPFNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVAST
                     VQEGVMRNHN"
     gene            30576..31148
                     /locus_tag="RH057_001790"
     CDS             30576..31148
                     /locus_tag="RH057_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3128171.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINKGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
     gene            complement(31179..31490)
                     /locus_tag="RH057_001791"
     CDS             complement(31179..31490)
                     /locus_tag="RH057_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HDV3128172.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            31653..31749
                     /gene="ffs"
                     /locus_tag="RH057_001792"
     ncRNA           31653..31749
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="RH057_001792"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            31859..32929
                     /locus_tag="RH057_001793"
     CDS             31859..32929
                     /locus_tag="RH057_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001548253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3128173.1"
                     /translation="MSLMLSRHGIWYYRKVNVLPCGKRREFRRSLGTRSKTEATKRSL
                     RFADRPWLITETSTTTIDLIQSPNSDLSLSELKLHAEQYVEFKSREVCEREVSSIKRC
                     IDKYFSFTEQVFKRSRAAEFISGLDVAIPTKNKYVKKISGFFRWLKHRTDYDFQNPFE
                     GLLQKDREPVSQKREAYTSAQIRCLENGISTLPDWKIWIILLGRFTGMRANEICQLYH
                     GDILKNEGRWCIRIDDRHDGQVVKTENSKRTIPIHNKLIDIGFLDYVQKQKGRLFPQL
                     TFYKGSYSHYFTRWFSGYRKKLGVPEFHSLRHYIATKLKNAGVVEHYAAAFLGHADNS
                     ITYRRYGKSVAVSQLYDLLEHI"
BASE COUNT         8391 a         7990 c         8834 g         7754 t
ORIGIN      
        1 tgttaactaa aagaatatta ttttctgtaa tgctgatagt ttcaccaagc gtggtggcca
       61 gcgagaaagc gcatgaattg tatgacagca tatacggtgg taaacctgct cccgatgtta
      121 taaatacact gcataaaatg gctgaatcgg gagatatcga cgcgcagagc ctgttaggct
      181 gggaatatta tcagcctcgt tatgatacca aacctgatgt tcaggaagcg attaaatggt
      241 tcgagttagc ggctaaacaa ggtgacagag aagctccgtt agcgctgggg ggtatctact
      301 acgacggtga gcaggtgcgg gtggattatg ccaaagcgta tgcactattt aatcaggccg
      361 cgcagcacgg tgggaattta gcatggtcca ggttgggcat tatgtacgcc aatggtcagt
      421 atgttgaggt agattgtaag aaagcgaagg aatatcttga taaaggtgtc cacatttatg
      481 gtggcccaga agactttctg gctacttgtc gaaaagacat gattgacaga aaaacggttg
      541 acgatacgtt acccgtgatt acggttaccc gttctgggat gcgagataat tttttagata
      601 aaggtttttc ctgcatggat agcctcttcg ccaccaccaa taaattaggc gaagtggcca
      661 atcttcgcgt gacattcagc attcgtcgcc catccggtaa ggagattaac caaacggttg
      721 gcttcgcgcc tttcggttta aaccggctga atattagctt tactgattac ctatttggtt
      781 catttaccag taattcgtct cttattcttt ataaaccgga atttgagcga aaatcttgtg
      841 caactgtaag gaccacaatc gttgccgcaa cggcaaccat taacggcaag gatgtggagt
      901 tgctgaaagc gggggcaatt gaacaaaagt ggtaacgctg ctcagccccc ggctgaggca
      961 aaacttgccc tggcgggtgg cgataataat ctaagccgcg agaatatgct gaatcgccaa
     1021 aaacataggt acagcaccaa aagcatttgg tgtcgctgaa agctgaacaa agccgagacg
     1081 agcataaaaa ttaatggcgg cagggtcagc atcaagataa acccctttaa ttggtaatgc
     1141 ctggtgaatt atttttacat gttcaaaaaa atcacatagt aggtcctgac caaagccccg
     1201 cttttgatac ttccgtgcta cccccaacat gactaatctg acaacaccaa tttctgaagg
     1261 ttgtgaaccc tgaaggacgc cagaaacgcg ttgtttttcc agcgaatatg ccgtaaaagt
     1321 acagatgccg atcagttcac cactttgtct gtcaataagt gctttagccg cacagtcgct
     1381 attacgcaca cttttcttta gcgatgcgcg tacaaattta tcgataacag ggttaccgca
     1441 atcaaactgt ttttgcccag gataagtaat atcagactga taattacagg agaggactat
     1501 gggcagagta atctcttcat gcttgtcgac catcgctgtt tttccttttc attaacgcct
     1561 tgagggcgag cgtcggttga gcaggttcag cgatgagttg gttcatcagt tcccagcttt
     1621 gattcgaaag ctcacggcga cgttggttat caagcacact ttcggcgcgt tccatagcag
     1681 cgctcaaaat aaaagcgctt aaatctaccc caacggcttg cgcagcttca cgaagtttgt
     1741 ttttggcgtc gggactagtt ttgagttcga cccgcgcatt tttcagcgtt ttatcgacat
     1801 cgggtagagt ggctgttttt ccaggcatta tgacctcttt gattaactga atgaaatcgt
     1861 agggagtcga tctcatacat aatacggcta tcaaccgtat gattaattta tgttcatctg
     1921 ctcactctgt caagatatac ggttattagc cgtatctctg atctcagaca ataaaaaagg
     1981 tgccgtagca cctttttaat agagaggttt tgttaccaca cagcagccag cagcgtatgc
     2041 gagtccggta ccataaaatc aacggacatc atcaccgaga gggcagtgat ggcgatgata
     2101 gagaagccga acagcttgcg cgcccagatt ctgtcatcag caactttata accgcgcaga
     2161 gccataccta accaccagac gctaaccgcc gcggcgacca ccagatattt atatccagcg
     2221 taaccgccaa gagagagcat cagcgtggca acggcaaagg cgatgatata cagcgtgatg
     2281 tgattcttcg ccaccgaaat gccttttacc actggcaata ccggaatgtt tgccgcctgg
     2341 taatccttaa agcggaaaat ggcgatggca taggagtgag gcatctgcca caggctgaag
     2401 atagccagca ggatcgctgc gccgctatcg aactcgccgg ttactgcaca gtagccaatc
     2461 accggcggcg cagcgccgga gagcgaacca atcaatgtgc cgtagactga gtggcgtttc
     2521 atgtacaggc tatagacgcc gacataaacc acaaagccca tcacccccag ccagcaggcc
     2581 agcggattcg cgccaaacca cagcagcata aagccagcaa tacccagcaa ggtggcgtac
     2641 accagcgaga cagcaggaga gatcaggcct ttaaccagca cccgattctt cgtcctttcc
     2701 atctttctgt cgatatccct gtcgatgtag ttgttaaaca cacaacccga cgccacaacc
     2761 agtgacaccc caaccagcgt gtagataaac aggggataat caatgctgcc ctttgaggcc
     2821 agcaggaatc ccccaatcac cgagatcagg ttgccaaaga tgatgcctgg tttcgttact
     2881 tgcaggtatt gcttaaacat cataaccgcc gctcttagtg catcatcatg ttgtagttga
     2941 ggttccacat aatccagatg gagcctacaa ccaggatagc gatgattagc acggtgaaga
     3001 caaacgccgt catgttccag ccttcatctg atttggtatt catgtgcagg aagcacacca
     3061 gatgcaccag aacctgtacc actgccattg ccaggattgt tcccagaatt acggccggag
     3121 aggcagcccc tgtcatcacc atccagaacg gaatcaccgt cagaatgatc gacaggataa
     3181 agcctgtcat gtaggttttt acgctgccat gggacgcgcc gctgtgatcg gtagaatgac
     3241 tcattacatc gcccccatca gataaacaac agtgaacaca cagatccaaa ccacatccag
     3301 gaagtgccag aacaggctca ggcacatgat gcgggtacgg ttagtgctgg tcaggccgcg
     3361 acgagcgatt tgtaccatca gcaccgccat ccagataaga ccggaagtga cgtgcagacc
     3421 gtgcgtgccg accagcgcaa agaacgctga caggaagccg ctgcgatccg gacccatgcc
     3481 gttaacaatc aggtgatgga attcgtagat ttccatcccg ataaatccgg caccaaacaa
     3541 ccaggtcaac gccagccagg agataacctg gcttttgttg tttttgtaca tggcgatagc
     3601 cgccatgccg taggtgatgg agctgaacaa cagcaagaaa gtttcaacca gaacgaacgg
     3661 cagttcgaaa atgtccttac ctgtcgggcc gcctgcggtg ccgttcacca gaacggcata
     3721 ggtagcaaac aagattgaga acagaatgca gtcgctcatc aggtagatcc aaaatccgaa
     3781 gattttggtt ccgcctgcat cgtggtgccc gtgttcgtgc gcgtgggcag tcgcgtgcgt
     3841 caaagtatca gttgccattt ttcagccctg ccttagtaat ctcatcgaaa tgctggtttt
     3901 ccagtttttc gatttctgcc accggcacgt agtaatccac gtcctcgtcg aagcttttca
     3961 cgatccaggt gatgatcatg cctgcgaagc caacaatcgc cagccaccag atatgccaga
     4021 tcatggcgaa accgaagatg gtggagaaag ctgcaatgac gatacctgca ccgctgtttt
     4081 tcggcatatg aatttcttca tagtggtcag gctttttata cgcttcgcct ttctctttca
     4141 tttcccagaa tgcatcacgc tcgtgaacgt gcggcacaac ggcaaagtta tagaacggag
     4201 gcggggaaga ggttgcccac tccagcgtac ggccacccca cgggtcgcca gtcaggtcac
     4261 ggttctggtc gcggtcgcga atagaaacgt acatctgaat aacgaggcag agaataccca
     4321 gcgcaatcag tactgcaccg ctagctgcaa tcatcagcat ggtgtggaac tgcgggtcaa
     4381 tctgctggct caaacgacgg gtcatgccca tgaagcccag cgcatacagc ggcataaagg
     4441 caacgaagaa gccgatgatc cagaaccaga acgcgcgttt accccaggtt tcattcagtt
     4501 tgaaaccgaa ggctttaggc caccagtagg tcatccctgc gaagcagccg aagaccacgc
     4561 cgccgatgat cacgttatgg aagtgcgcaa tcaggaacag gctgttatgc agaacgaagt
     4621 ccgcgcccgg tactgccagc agcacgccag tcatcccgcc caccgagaaa gtgacgataa
     4681 aaccgatggt ccacagcatc gcagaatgga acacgatgcg gccctgatac atggtgaaca
     4741 gccagttgaa gatcttcacc ccggtcggga tggcgataat cattgtggtg ataccaaaga
     4801 aggcgtttac gttcgcgccc gcacccatcg taaagaagtg gtgcagccaa acgatgaacg
     4861 acagcacggt gatacagacg gttgcccata ccagcgaggt ataaccaaac agacgtttac
     4921 gcgagaaggt tgccgcaatt tcggagaaca caccgaaaac aggcaggatc aggatgtaaa
     4981 cttccgggtg gccccaggcc caaatcaggt tgatgtacat catcatgttg ccacccatat
     5041 cgttggtaaa gaaatgggtg cccagatagc gatccagggt caacaacgcg acggtaaccg
     5101 tcagaattgg gaaggaagca ataatcagta cgttcgcgca cagtgatgcc caggtaaata
     5161 ctggcatctt gaacatggtc atgcccggtg cgcgcatctt cagaatggta acgaagaagt
     5221 tgataccggt aagcgtcgta cctataccgg atagctggag actccatatc cagtaatcga
     5281 caccgactcc cggactgtac tctattcccg atagcggtgg ataggccagc cagccggtct
     5341 gcgcaaattc gcccacgccg agagaaacgt taaccagaat cacaccaaca acagtaaacc
     5401 agaagcttaa gttgttgagg aacgggaacg caacgtcacg cgcgccgatc tgcagcggaa
     5461 ccaccaggtt catcagaccg ataacgaaag gcatcgccac gaagaagatc ataatcacgc
     5521 cgtgcgcggt gaagatctga tcgtagtggt gaggtggcag gaagcccgct tcgcccgccg
     5581 aggcaagagc ctgctggcta cgcatcataa tggcgtcagc aaaaccacgc agcaacatca
     5641 caatcgccac gatgatatac atgataccga ggcgtttatg gtcgacggag gtcagccact
     5701 ctttccacag gtaggtccac ttaccgaagt aagtgatcag gccaacgagc gccagacctc
     5761 ccaaaataat gccagcgatc gtaaccatga cgataggttc atggaacggg actgcatcaa
     5821 gtgataattt tccgaacatc tttattcttc ctcaacccct ttaatgggcg gattccgcgt
     5881 ggctcatgtc catgccttcc ataccttcgt gtgcactgtg ctcaccttct ggctgggtca
     5941 tgtccatgct cttaccgtga gccataaact tgttaatcac atcggcaaac aagtctggtt
     6001 tcacgttgga gaaatattcc acctggttgt attcgctagg cgcggccagt ttttcgaacg
     6061 cagccatgtc agacatggtg ttcggcgact gtttcgcttt tgcgacccat tggtcgaatt
     6121 cggcgcgatc cggtgttgca atagctttga acttcatgcc tgagaagccc ggcccgctgt
     6181 agctggcgga gataccgtca taagtaccgg gttcgttggc gatcagatgc aggcgagtct
     6241 gcataccggc catggcataa atctggctac ccagacgcgg aatgaagaag gagttcatca
     6301 cggagttgga ggtcactttg aagtacaccg gagtgttcgc agggaaagcg atttcattca
     6361 cggtagcaat gccctgttcc gggtagatga agaaccattt ccagtccatg gaaaccactt
     6421 cgatggtaat gggcttctcg tcgtgtgcca gcggcttgct aggctcaaga gcgtgagtgg
     6481 ttttccaggt cagtaccgca aggaagatga tgattaagat aggtaccgtc cagaccacag
     6541 cttccacttt attggagtgt gaccagttcg ggctgtactt agcatcttta ttgctcgcac
     6601 gatacttcca ggcgaaacca acagccatca agattgcggg aataacgaca atcaacatca
     6661 ggccaaatgc cgtcagtatc agtgaacgtt gctccagacc aatctgtcct ttgggatcta
     6721 acagcgcaga attacagcca ctgagcaata cagtgcctgc aaataatgac aaccatccca
     6781 aacttttatt gtatttcctg agtctcattt aacgacctca attccacggg atctggtggc
     6841 gtttaaagtg tgtgggcatt ttacgggaag gttacattac tgtaaacatg attaaattcc
     6901 tgttacttgg atttggctgt caggtcacat atgttgcaaa acatatcaag ttgattttaa
     6961 gaagctgttg caaaagggcg ttataacgaa aggggagtgc agaaggagtt tggacgggca
     7021 attggtataa ccaatctgga ataaaacaat tgtttaacaa ataattatca ttatggtgac
     7081 aattgggtgg gggaaaataa agactcatta gctgaatcgt gtaagaaaaa atagaattca
     7141 taaattgcgt caataatttc caaacgctaa gccgcacaaa agaacaaata ttattaagcg
     7201 atactgatgt ttatttagcc gtaataatta cggcgagtga ttactacagc taaataatat
     7261 ttacagatta cgtcagatgc gtttttcgta gcgccagata atccagcaaa ccaccaagca
     7321 cgacgccaga aagggcgact aaaaccccga cttccagcag agcaggcagg aaagagaagt
     7381 gcgtcaaatc cagcgcgtcc atcgtcaaca gcagtaacca cacggccaac aggctgacgc
     7441 ccgccgccag tgtccacatg gcaaaggcat aacctgccgg atattcggta cgggagataa
     7501 agttgtcatt tactcgtgta tattcaagcg tctggcggca aaccagcagc aaaataagcc
     7561 ccggtacggc agcggcgacg gagaatagat agaatgtcga ccagccgtgt gcttcaacaa
     7621 accaacccgc cacggggccg acataaactc gccctacagc ggaaagcgct gagagcaggg
     7681 caaattgagt tgcggaaaat gacttattac acagcgtcat taacagcgcg acaaaggctg
     7741 atgtgcccat cccgccacag aggttttcga aaaagacggc tgcgcccatg ctgtagagat
     7801 gcttatcagt aatcgacagc agccagtaac cagcgttaga cgcaccttgt aaaatgccga
     7861 aaatcagcag cgcccggaac agcgacaggc gctgcatcaa aatcccaccg tacaatgcgc
     7921 ccacaatggt cgccagtaag ccaagcgttt tgttaaccac gcctacttca cccgcatcaa
     7981 acccgacgcc gcgaatcaaa aacgtggttg tcaggctcat ggcgaatgcg tcgcccagct
     8041 tatacagcac gataagaagc aaaataagcc aggcattatt gcgaccaaag aaatctcgca
     8101 gaggtgcaac aaccgcttgt tccagcgttt ttggcacagg aatggtgtcg gtcggttctg
     8161 gtgcaagcaa cgtcgcgata atacaaggga tcaacagcgc cgccatcaac cagtacatgc
     8221 cctgccagcc cagccattta tctgccagcc acagggctaa gccgccggaa accagcatcc
     8281 ctaaacggta acccagcacg ctgattgccg cgcccgcacc acgttcttct gccgggagca
     8341 catcggtttt ccacgcatcg aagacaatat cctgggaggc cgagcaaaaa gcgatcacca
     8401 ccgccagtgc cgccatccag cggagttggg tgccaggttc gagaaaaccc atcgccgcaa
     8461 tggcgactaa taacaggatt tgcgtggcga gcagccaacc gcgccgccgc ccaaaaaatg
     8521 ggggcgtgta gcggtccatc agcggtgacc agaggaattt aaaaacgtaa gcctggccta
     8581 ccagagagaa gaaaccaatg gttttgagat caatattctc gaccgtcatc caggcctgta
     8641 aggtgccgga tgtcagggcg agcggtagcc cggaagcaaa acccaggatc agcaatatgg
     8701 ctgaacgcgg ctgttgaaaa atacgtaaat attgactgga catgggcgtc tacaggcccg
     8761 gtttgcaccg ggccagaggg cagaaattaa cgcgcgttct gcttgataaa ttcgtgaatg
     8821 ctggtgtcct gagacatatc agcgatggta tcggtcagca cactgttaac cgcatcggcg
     8881 atatttttgt tggaggcctg gaacgcacct tcaacgttgt agctggcacg atagtttttg
     8941 gtcattttgt tgccattctg cgcggtagcg atgatggcga tatccgcttt ggtagcgatg
     9001 ttgtagcgca cgttgccctg ggacacgtca gcatacagtt ggctaacgat gatttgcaga
     9061 ttaaccgggc cattcggacc aaccatgtaa ccacgcgcgg tcatctgttt ttccagcact
     9121 tcttgcagca ggaaacgcag atcgcgggag gcggtcaggg taacgatttg attatcgcgg
     9181 gtgacttttg ccagcgcctg atcggtacgc tgatcggcac cattaatgct tacggtgacg
     9241 cccatcaggc ttggatcctg ctgtggcagt gtaatcgtcg gggaaacttc aatagttgtt
     9301 ggcggttttg cgcatcctgc aagcataaac agagcaacta acgggaagag gatttttttg
     9361 aacatgttcg ggctctcaga gactcttaag cgtgtttggt aaaaattccc gccatcataa
     9421 cattgccaac ggcgagggga agtgggtaag gcatgtaaat tcatcatgtt gacgaaataa
     9481 tcgcccctgg caaaagaaac actgatgcga ggcctgtgtt tcaatcttta aatcagtaaa
     9541 cttcatacgc ttgacggaaa aaccaggacg aaacctaaat atttgttgtt aagctgcaat
     9601 ggaaacggta aaagcggcta gtatttaaag ggatggatga catctcagcg ttgtcggagg
     9661 agatatttca tgatgatacg tgagcggata gaagaaaaat taagggcggc gttccaaccc
     9721 gtattcctcg aagtagtgga tgaaagctat cgtcacaatg tcccagccgg ctctgaaagc
     9781 cattttaaag ttgtgctggt cagcgatcgt tttacgggtg aacgttttct gaatcgtcat
     9841 cgaatgattt acagtacttt agcggaggaa ctctctacta ccgttcatgc gctggctctg
     9901 catacttata ctattaagga gtgggaaggg ttgcaggaca ccgtctttgc ctctcctccc
     9961 tgtcgtggag caggaagcat cgcgtaaaaa cgcatttgca actgtcggcg cttttccagt
    10021 atgttgctaa agattttatg aaaaacggcc tgcgggccgt tttgttttgt ctggattttg
    10081 cgctttttgc ccagcattca gacgaaaatt gcccgggaat tgtgaaaaaa tacgcgacag
    10141 cgcgcaataa ccgttctcga ctcataaaag tgatgccgct ataatgccgc gtcctatttg
    10201 aatgctttcg ggatgattct ggtaacaggg aatgtgattg attataagaa catcccggtt
    10261 ccgcgaagcc aacaacctgt gcttgcgggg taagagttga ccgagcactg tgattttttg
    10321 aggtaacaag atgcaagttt cagttgaaac cactcaaggc cttggccgcc gtgtaacgat
    10381 tactatcgct gctgacagca tcgagaccgc tgttaaaagc gagctggtca acgttgcgaa
    10441 aaaagtacgt attgacggct tccgcaaagg caaagtgcca atgaatatcg ttgctcagcg
    10501 ttatggcgcg tctgtacgcc aggacgttct gggtgacctg atgagccgta acttcattga
    10561 cgccatcatt aaagaaaaaa tcaatccggc tggcgcaccg acttatgttc cgggcgaata
    10621 caagctgggt gaagacttca cttactctgt agagtttgaa gtttatccgg aagttgaact
    10681 gcagggtctg gaagcgatcg aagttgaaaa accgatcgtt gaagtgaccg acgctgacgt
    10741 tgacggcatg ctggatactc tgcgtaaaca gcaggcgacc tggaaagaaa aagacggcgc
    10801 tgttgaagca gaagaccgcg taaccatcga cttcaccggt tctgtagacg gcgaagagtt
    10861 cgaaggcggt aaagcgtctg atttcgtact ggcgatgggc cagggtcgta tgatcccggg
    10921 ctttgaagac ggtatcaaag gccacaaagc tggcgaagag ttcaccatcg acgtgacctt
    10981 cccggaagaa taccacgcag aaaacctgaa aggtaaagca gcgaaattcg ctatcaacct
    11041 gaagaaagtt gaagagcgtg aactgccgga actgactgca gaattcatca aacgtttcgg
    11101 cgttgaagat ggttccgtag aaggtctgcg cgctgaagtg cgtaaaaaca tggagcgcga
    11161 gctgaagagc gccatccgta accgcgttaa gtctcaggcg atcgaaggtc tggtaaaagc
    11221 taacgacatc gacgtaccgg ctgcgctgat tgacagcgaa atcgacgttc tgcgtcgtca
    11281 ggctgcacag cgtttcggtg gcaacgaaaa acaagctctg gaactgccgc gcgaactgtt
    11341 cgaagaacag gctaaacgcc gcgtagttgt tggcctgctg ctgggcgaag ttatccgcac
    11401 caacgagctg aaagctgacg aagagcgcgt gaaaggcctg atcgaagaga tggcttctgc
    11461 gtacgaagat ccgaaagaag ttatcgagtt ctacagcaaa aacaaagaac tgatggacaa
    11521 catgcgcaat gttgctctgg aagaacaggc tgttgaagct gtactggcga aagcgaaagt
    11581 gactgaaaaa gaaaccactt tcaacgagct gatgaaccag caggcgtaat ttacgcagca
    11641 taacgcgcta aattcgcaca aaggcccgtc accgccaggt ggtgggcttt tttttgtcat
    11701 gaattttgca tggaaccgtg cgaaaagcct ctttcggtgt tagcgtaaca acaaaagatt
    11761 gttatgcttg aaatatggtg atgccgtacc cataacacag ggactagctg ataatccgtc
    11821 cataaggtta caatcggtac agcaggtttt ttcaatttta tccaggagac ggaaatgtca
    11881 tacagcggcg aacgagataa ctttgcaccc catatggcgc tggtgccgat ggtcattgaa
    11941 cagacctcac gcggtgagcg ctcttttgat atctattctc gtctacttaa ggaacgcgtc
    12001 atttttctga ctggccaggt tgaagaccac atggctaacc tgattgtggc gcagatgctg
    12061 ttcctggaag cggaaaaccc agaaaaagat atctatctgt acattaactc cccaggcggg
    12121 gtgatcactg ccgggatgtc tatctatgac accatgcagt ttatcaagcc tgatgtcagc
    12181 accatctgta tgggccaggc ggcctcgatg ggcgctttct tgttgaccgc aggggcaaaa
    12241 ggtaaacgtt tttgcctgcc gaattcgcgc gtgatgattc accaaccgtt gggcggctac
    12301 cagggccagg cgaccgatat cgaaattcat gcccgtgaaa ttctgaaagt taaagggcgc
    12361 atgaatgaac ttatggcgct tcatacgggt caatcattag aacagattga acgtgatacc
    12421 gagcgcgatc gcttcctttc cgcccctgaa gcggtggaat acggtctggt cgattcgatt
    12481 ctgacccatc gtaattgatg ccagaggcgc aactgtgccg ctatacttat ccagggcggc
    12541 acaacgctgt aagcggcttg cgcctgagaa tggcatttgc gtcgtcgtgt gcggcacaaa
    12601 gaacaaagaa gaggttttga cccatgacag ataaacgcaa agatggctca ggcaaattgc
    12661 tgtattgctc tttttgcggc aaaagccagc atgaagtgcg caagctgatt gccggtccat
    12721 ccgtgtatat ctgcgacgaa tgtgttgatt tatgtaacga catcattcgc gaagagatta
    12781 aagaagttgc accgcatcgt gaacgcagtg cgctaccgac gccgcatgaa attcgcaacc
    12841 acctggacga ttacgttatc ggccaggaac aggcgaaaaa agtgctggcg gtcgcggtat
    12901 acaaccatta caaacgtctg cgcaacggcg ataccagcaa tggtgtcgag ttgggcaaaa
    12961 gtaacattct gctgatcggt ccgaccggtt ccggtaaaac gctgctggct gaaacgctgg
    13021 cgcgcctgct ggacgtcccg ttcaccatgg ccgacgcaac cacgctgacc gaagccggtt
    13081 atgtgggcga agacgttgaa aacatcattc agaagctgtt gcagaagtgc gattacgacg
    13141 tacagaaagc gcagcgcggg attgtctaca tcgatgaaat cgacaagatt tctcgtaagt
    13201 cagacaaccc gtccattacc cgagacgttt ccggtgaagg cgtacagcag gcactgttga
    13261 aactgatcga aggtacggta gctgctgttc caccgcaagg tgggcgtaaa catccgcagc
    13321 aggaattctt gcaggttgat acctctaaga tcctgtttat ttgtggcggt gcgtttgccg
    13381 gtctggataa agtgatttcc caccgtgtag aaaccggctc cggcattggt tttggcgcga
    13441 cggtaaaagc gaagtccgac aaagcaagcg aaggcgagct gctggcgcag gttgaaccgg
    13501 aagatctgat caagtttggt cttatccctg agtttattgg tcgtctgccg gttgtcgcaa
    13561 cgttgaatga actgagcgaa gaagctctga ttcagatcct caaagagccg aaaaacgccc
    13621 tgaccaagca gtatcaggcg ctgtttaatc tggaaggcgt ggatctggaa ttccgtgacg
    13681 aggcgctgga tgctatcgct aagaaagcga tggcgcgtaa aaccggtgcc cgtggcctgc
    13741 gttccatcgt agaagccgca ctgctcgata ccatgtacga tctgccgtcc atggaagatg
    13801 tcgaaaaagt ggttatcgac gagtcggtaa ttgatggtca aagcaaaccg ttgctgattt
    13861 atggtaagcc ggaagcgcaa caggcatctg gtgaataatt aaccattccc atacaattag
    13921 ttaaccaaaa aggggggatt ttatctcccc tttaattttt cctctattct cggcgttgaa
    13981 tgtgggggaa acatccccat atactgacgt acatgttaat agatggcgtg aagcacagtc
    14041 gtgtcatctg attacctggc ggaaattaaa ctaagagaga gctctatgaa tcctgagcgt
    14101 tctgaacgca ttgaaatccc cgtattgccg ctgcgcgatg tggtggttta tccgcacatg
    14161 gtcatcccct tatttgtcgg gcgggaaaaa tctatccgtt gtctggaagc ggcgatggac
    14221 catgataaaa aaattatgct ggtcgcgcag aaagaagctt caacggatga gccgggtgta
    14281 aacgatcttt tcaccgtcgg gaccgtggcc tctatattgc agatgctgaa actgcctgac
    14341 ggcaccgtca aagtgctggt cgaggggtta cagcgcgcgc gtatttctgc gctctctgac
    14401 aatggcgaac acttttctgc gaaggcggag tatctggagt cgccgaccat tgatgagcgg
    14461 gaacaggaag tgctggtgcg tactgcaatc agccagttcg aaggctacat caagctgaac
    14521 aaaaaaatcc caccagaagt gctgacgtcg ctgaatagca tcgacgatcc ggcgcgtctg
    14581 gcggatacca ttgctgcaca tatgccgctg aaactggctg acaaacagtc cgttctggag
    14641 atgtccgacg ttaacgaacg tctggaatat ctgatggcaa tgatggaatc ggaaatcgat
    14701 ctgctgcagg ttgagaaacg cattcgcaac cgcgttaaaa agcagatgga gaaatcccag
    14761 cgtgagtact atctgaacga gcaaatgaaa gctattcaga aagaactcgg tgaaatggac
    14821 gacgcgccgg acgaaaacga agccctgaag cgcaaaatcg acgcggcgaa gatgccgaaa
    14881 gaggcaaaag agaaagcgga agcagagttg cagaagctga aaatgatgtc tccgatgtcg
    14941 gcagaagcga ccgtagtgcg tggttatatc gactggatgg tacaggtacc gtggaatgcg
    15001 cgcagcaagg tcaaaaaaga cctgcgtcag gcgcaggaaa tccttgatac cgaccattat
    15061 ggtctggagc gcgtgaaaga tcgcatcctt gagtaccttg cggttcaaag ccgtgtcaac
    15121 aaaatcaagg gaccgatcct ctgcctggta gggccgccgg gggtaggtaa aacctctctt
    15181 ggtcagtcca ttgccaaagc caccgggcgt aaatatgtcc gtatggcgct gggcggcgtg
    15241 cgtgatgaag cggaaatccg tggtcaccgc cgtacttaca tcggttctat gccgggtaaa
    15301 ctgatccaga aaatggcgaa agtgggcgtg aaaaacccgc tgttcctgct cgatgagatc
    15361 gacaaaatgt cttctgacat gcgaggcgat ccggcctctg cactgcttga agtgctggat
    15421 ccagagcaga acgtagcgtt cagcgaccac tacctggaag tggattacga tctcagcgac
    15481 gtgatgtttg tcgcgacgtc gaactccatg aacattccgg caccgctgct ggatcgtatg
    15541 gaagtgattc gcctctccgg ttataccgaa gatgaaaaac tgaacatcgc caaacgtcac
    15601 ctgctgccga agcagattga acgtaatgca ctgaaaaaag gtgagctgac cgtcgacgat
    15661 agcgccatta tcggcattat tcgttactac acccgtgagg cgggcgtgcg tggtctggag
    15721 cgtgaaatct ccaaactgtg ccgcaaagcg gttaagcagt tactgctcga taagtcatta
    15781 aaacatatcg aaattaacgg cgataacctg catgactacc tcggtgttca gcgtttcgac
    15841 tatggtcgcg ctgataacga aaaccgtgtc ggtcaggtaa ccggtctggc gtggacggaa
    15901 gtgggcggtg acttgctgac cattgaaacc gcatgtgttc cgggtaaagg caaactgacc
    15961 tataccggtt cgctcggcga agtgatgcag gagtctattc aggcggcgtt aacggtggtt
    16021 cgtgcgcgtg cggaaaaact ggggatcaac cctgattttt acgaaaaacg tgacatccac
    16081 gtccacgtac cggaaggtgc gacgccgaaa gatggtccga gtgccggtat tgctatgtgc
    16141 accgcgctgg tttcttgcct gaccggtaac ccggttcgtg ccgatgtggc aatgaccggt
    16201 gagatcactc tgcgtggtca ggtactgccg atcggtggtt tgaaagaaaa actcctggca
    16261 gcgcatcgcg gcgggattaa aacagtgcta attccgttcg aaaataaacg cgatctggaa
    16321 gagattcctg acaacgtaat tgccgatctg gacattcatc ctgtgaagcg cattgaggaa
    16381 gttctgactc tggcgctgca aaatgaaccg tctggcatgc aggttgtgac tgcaaaatag
    16441 tgacctcgcg caaaatgcac taataaaaac agggctggca ggctaattcg ggcttgccag
    16501 cctttttttg tctcgctaag ttagatggcg gatcgggctt gcccttatta aggggtgttg
    16561 taaggggatg gctggcctga tataactgct gcgcgttcgt accttgaagg attcaagtgc
    16621 gatataaatt ataaagagga agagaagagt gaataaatct caattgatcg acaagattgc
    16681 tgcaggggct gatatctcta aagctgcggc tggccgtgcg ttagatgcta ttattgcttc
    16741 cgtaactgaa tctctgaaag aaggggatga tgtagcactg gtaggttttg gtacttttgc
    16801 cgttaaagag cgtgctgccc gtactggccg caacccgcag accggtaaag agatcaccat
    16861 cgctgctgct aaagtaccga gcttccgtgc aggtaaagca ctgaaagacg cggtaaacta
    16921 agcgttgtcc ccggtgggga tgtgacgaag ttcaagggcg catctactga tgtgcctttt
    16981 ttatttgtat tcggtgactt tctgcgtctt gtgggctgac aattgccccc gtttcttgtc
    17041 acaataggcc tttgcgcgca tcgatacgtt gcgtgaggta cacagtcatc tacagcggag
    17101 tgttgttaca ccatgatgga cagcttacgc acggctgcaa acagtctcgt gctcaagatt
    17161 attttcggta tcattatcgt gtcgttcata ttgaccggcg tgagtggtta cctgattggc
    17221 ggaggcaata actacgccgc aaaagtgaat gaccaggaaa tcagccgtgg gcagttcgag
    17281 aacgccttta acagcgagcg taatcgcatg cagcaacagc tgggcgatca atactccgag
    17341 ctggcagcga atgaaggcta tatgaaaacc ctgcgtcaac aggtgctgaa tcgtctgatc
    17401 gacgaggcgc tgttggatca gtacgctcgt gagctgaaac tgggtatcag tgatgagcag
    17461 gttaaacagg cgattttcgc gaccccagcc ttccaggttg acggcaaatt tgataacagc
    17521 cgctataacg gtatcctcaa ccagatgggg atgaccgccg atcagtacgc ccaggcgctg
    17581 cgtaaccagc tcactaccca acagctgatt aacggtgttg ccggtaccga ttttatgctg
    17641 aaaggtgaaa ccgacgagct ggcggcactg gtcgcgcaac aacgcgtggt gcgtgaggcg
    17701 actatcgatg ttaacgcgct ggcggcgaag cagcctgtga ccgaacagga gattgccagc
    17761 tactacgaac aaaacaaaaa caatttcatg acgccggaac aattccgcgt gagttacatc
    17821 aagctggatg ccgcaacgat gcagcaaccg gttagcgatg cggatatcca gagctactac
    17881 gatcagcatc aggatcaatt cacccagccg cagcgtaccc gctacagcat catccagacc
    17941 aaaactgaag atgaagcgaa agcggtactt gatgagctga ataaaggcgg tgattttgct
    18001 gcattagcca aagaaaaatc tgccgatatt atctctgccc gtaacggcgg cgatatgggt
    18061 tggttagaag atgccactat ccccgacgaa ctgaaaaatg ctggtctgaa agaaaaaggg
    18121 caactgtctg gtgtcatcaa atcttcggtc ggtttcctga ttgtacgtct ggacgacatt
    18181 cagccagcga aagtgaaatc gttagacgaa gtacgtgacg acattgcggc gaaagtgaaa
    18241 cacgaaaaag ccctcgatgc gtactatgcg ctgcagcaga aagtgagcga tgcggcaagc
    18301 aacgacaccg agtctctggc cggtgcagag caagctgccg gcgttaaagc cactcagacg
    18361 ggttggttta gcaaagataa cctgccggaa gagttgaact tcaagccggt tgccgacgct
    18421 atctttaacg gcggtctggt aggtgaaaac ggcgcgccgg gcatcaactc tgacatcatc
    18481 accgtagacg gcgaccgcgc attcgtgctg cgcatcagcg agcacaaacc ggaagcggtg
    18541 aaaccgttgg cagatgttca ggaacaagtt aaggcactgg ttcagcacaa caaagctgaa
    18601 caacaggcga aagtggatgc tgagaaactg ctggttgatt tgaaagccgg caaaggtgcg
    18661 gaagctatgc aggctgccgg tctgaaattt ggcgagccga aaatcttaag ccgttccggt
    18721 cgtgacccga ttagccaggc ggcgtttgca ctgccactgc cagcgaaaga caaaccgagc
    18781 tacggtatgg cgaccgatat gcaaggtaat gtggttctgc tggcgctgga tgaagtgaaa
    18841 caaggttcaa tgccggaaga tcagaaaaaa gcgatggtgc agggtatcac ccagaacaac
    18901 gcacaaatcg tctttgaagc tctgatgagt aacctgcgta aagaggcgaa aatcaaaatt
    18961 ggcgatgcgc tggaacagca ataatcctga agccgcctcg caaaaaaatg cgttgcagct
    19021 gtaacaagaa aaggtcgctt tcgcggcctt ttccatttct gaacattgcc atttgtttac
    19081 tgttttcaat cccgttaagg tgattccact gttaacaaac aaggagaaaa cagtatgaaa
    19141 cacggaatta aagcactgct cattaccctg tccctggcct gtgccggaat gtctcatagc
    19201 gcgctggcgg cagcttctgt ggcgaaaccg acggcggtag aaaccaaagc ggaagctcct
    19261 gcagcacaaa gtaaagcagc agtaccggcg aaagccagtg acgaagaagg cacccgggtc
    19321 agcattaata atgccagcgc ggaagagcta gcccgcgcga tgaatggcgt tggcctgaag
    19381 aaagcgcagg cgattgtcag ttatcgcgaa gagtacggtc cgtttaaaac ggtggaggat
    19441 ctaaagcagg tgccggggat gggcaattcg ctgctggaac gtaatctggc ggtattaacc
    19501 ctgtaattaa tttgcatagt ggcaattttt tgccagactg aagaggtcat accagttatg
    19561 acctctgtac ttataacaac aacgtaaggt tattgcgcta tgcaaacaca aatcaaagtt
    19621 cgtggatatc atctcgacgt ttaccagcac gtcaacaacg cccgctacct tgaatttctc
    19681 gaagaagccc gctgggatgg gttggaaaat agcgacagtt ttcagtggat gacggcccat
    19741 aacatcgcct tcgtcgtggt caatatcaat attaactatc gtcgcccagc ggtattaagc
    19801 gacctgttaa ctattaccag tcagttgcag caattaaacg gtaaaagcgg catcttaagc
    19861 caggtcatta cactggagcc ggaagggcag gtggtagcgg atgcgcttat tacgtttgtt
    19921 tgtattgatc ttaaaacgca gaaagcatta gctctggaag gggaattgcg cgaaaagctg
    19981 gagcagatgg ttaagtaaac gttttgtggt gccggatgct caagccgcat ccggcgacac
    20041 ccggaataat tacctcaacc cggttttctg cttcatcgct gccatcaccg tcggtttatc
    20101 ggccagataa tgattcaaac cgttggcgcg taaattacat gccgcacaat gaccgcaacc
    20161 gtcgccttta atgccgttat agcaggtcaa cgtttcgtta cggactaaat ccagtttgcc
    20221 gtaataatct gccagcgccc aggtttccgc tttatcaatc cacatcagcg gcgtttcaaa
    20281 acgaatatct ttcgccatgc ccaaactgac ggcatggttt agtgctttca caaactcatc
    20341 gcggcaatcc gggtagccgg agaaatccgt ttcacagacg ccggtaatta cggcttctgc
    20401 ttttacctga tacgcatata ttgccgccag cgtcaggaac aaaatattac gccctgggac
    20461 aaacgtattc gggatgccat cggcttcagg ttcataatca ggcaccggaa tgctgtcacg
    20521 tgtcaggctg ctgaccgcca gctcgttgag cagcgttaca tcaagaacct tatgcgcgcg
    20581 tgctcccagt ttcagcgcca gttcgcgtgc cacgtcgatt tctgcgcgat gtcgctgacc
    20641 gtaatcgaac gtcacgcaat ggacttcatc atattgttgt aatgcctgca ccagacaggt
    20701 ggtggaatcc tggcctccac tgaacacaac gacagcacgt ttcatagata atcctgcttg
    20761 aacaataaaa gcgttatggt aacgcctgcg attaacccgg accagctatt cattgcgacg
    20821 gcgcgggaag ccaggcttcg gtaaattcaa accagccgcg cggtgtcagt cgcacaccgt
    20881 tcacgccggg aggggcacta atgcgatagt gatagttgaa cagcggcgtc agcgtcgcgt
    20941 ctgccattaa ctggctaaaa accgctttca gggcattaaa tcggttttcc tcatcaggca
    21001 ttacttgcac cgcgtccagc gtcgattgca gatgtgcata tgctggagcg tcgaaaacat
    21061 gtggccatag tggatcgcaa cgtagccatt gctccagagt atattccggt gcttcgccaa
    21121 ttaatctgtc gcccatcatg aggtctgcgt gtgccaatag ggtcgtgtcg tcccagtttt
    21181 ttgcgttatg aaaaataatt gtaagttcac agccttccgc tgccagtgtc gcctgtaggc
    21241 gttctgccat ggtatgaagt tctatcggta ggtgataaac cagcgtcaag gttttcggta
    21301 gtttgacttc atccggtact tgccattgcg gaatagtcca gcctggcagt aatgcatgac
    21361 tggcggtgat caggttttct cctacttcta acgtttgtaa taaaccggat tgatgaataa
    21421 tggagatcac ttttcgcgcc tgccagaggg agagtcgggg acttttgcgc aacgttaaat
    21481 agcaaaaacc taaactgatg ccactactga cctggctgac ccgttgcagc tcctccggtt
    21541 tgccgatggt gatttgcacg ggatgccgac agctggttcc caaatctttt tcgaaaagcg
    21601 gcggagttat ccagtactca accgctttaa gcagtggatg acgtaaatgg taataatcat
    21661 ggctttccag gcgcaccagt tccgctgtga attgtgttaa gcgaaaagga cccgtgccga
    21721 tcagtgggaa ttgcggatgc gccagatggc tacaatagct cgccagccgg tgcgcaagcc
    21781 agtaatcagg gcgatgtaaa aagaaggtca gacactgcgg atgggtgact tcaatacgct
    21841 tcacgctaat aaataattga tccagtgctg gcagttgtaa cagcatcaat aatcgctggt
    21901 gtaagtgtga ggcttttact gcatcgccgt tatgccagtg tagggttgaa cgaagataaa
    21961 agtcccagcg taacccgtca gtagaggttt cccagtgatg cgctaaatcg ccaatcgggc
    22021 gctgggtatt attatcgaag cgggtcaggc cggaaaatat ctgtccggcg agatgctgct
    22081 cggcacggcc gggcaaaaag cctggttgta gcggttcgag cgggcgatag tagggaatac
    22141 gcaacgtggg tgtatcgttt tgccattgtc cgcccataaa cggctgtaac agagtgcgca
    22201 gctcacctgg ggccagttgc gccagctcca gcacatcttg ctgctttccg gtttccagtg
    22261 cctgttccat catcgcattg cgtagcgatt ccggcgtgac cagaaagcgt aattgtccgc
    22321 gctttccgcg tcctgactgc gcctgccact ccagccatcc cgcctcctgt gcctgacgca
    22381 acagcgtacg aacatggcgt tcgctgcaaa aacagcgttc ggccagttca ttgacggtga
    22441 cagcttgcgg ctttccggcg gaaggttgcc acagacgttg atactggtta agacggttga
    22501 gcaatcgcat ataaacccgg aacaatatta tttaactatt cactattact tccgtatata
    22561 tcaggtgata ctcaatcacc attaaccgtg tcacagagtg gagaaagaaa tggctcgtct
    22621 ggcagcattt gatatggatg gcactttatt gatgcctgac catcatttag gtgagaaaac
    22681 cctctctact ttggcgcgac tgcgtgaacg cgacattacc ctcacttttg ccacggggcg
    22741 tcatgcgctg gagatgcagc atattctcgg ggcgctatcg ctggatgcat atttgattac
    22801 cggcaacgga acgcgcgtgc attctctgga aggtgaactt ttacattgtg atgatttacc
    22861 tgcggatgtc gcggagctgg tgctgtatca gcaatgggat acccgagcca gcatgcatat
    22921 cttcaatgac gacggttggt ttaccgggaa agagatccct gcgttgttgc aggcatttgt
    22981 ctatagcggt tttcgttatc agataatcga tgtcaaaaaa atgccactcg gcagcgtcac
    23041 caagatctgc ttctgtggcg atcacgacga tcttacacgc ttgcagatcc agctatacga
    23101 agcattaggc gagcgtgcac atttgtgttt ttccgccacg gattgcctcg aagtgctgcc
    23161 ggtgggctgc aataaaggcg ctgcattgac ggtgctgacc caacatttag gtttatcgtt
    23221 gcgcgattgc atggcctttg gtgatgcgat gaacgatcgc gaaatgttag gcagcgtcgg
    23281 tagcggcttt attatgggca atgcgatgcc gcaactgcgc gcggagctcc cgcatttacc
    23341 ggtgattgga cattgccgaa atcaggctgt ctctcactat ttgacgcact ggctggacta
    23401 tccacatcta ccttattccc ccgaataacg agatcccttc cagcaccggg caattgcccg
    23461 gttttttttg cgttgaattt gtcattttgt gccgtggtgt ttaaaccgca cagaataaat
    23521 tgtcgcgatt tcacctttaa aatagaatta aaagagaaaa aaattctccg tggaagggct
    23581 atgttagata aaattgaccg taagctgctg gccttactgc agcaggattg caccctctct
    23641 ttgcaggcac tggctgaagc cgttaatctg acaaccaccc cttgctggaa gcgcctgaaa
    23701 cggctggagg acgacggtat ccttatcggc aaagtcgccc tgctggatcc ggaaaaaata
    23761 ggcctcggcc tgaccgcctt tgtgctgata aaaacgcaac atcacagcag cgaatggtat
    23821 tgccgctttg tcacggtggt taccgaaatg ccagaagtgc tggggttctg gcgcatggct
    23881 ggtgaatacg attatctgat gcgcgtccag gttgccgaca tgaaacgcta cgacgagttt
    23941 tataagcgtc tggtaaacag cgtgccgggg ctgtcggacg tcacttccag cttcgcgatg
    24001 gaacagatta aatacaccac ttctttaccc atcgaataaa tatccagaat caggtcagga
    24061 cacaacgcgt gcgattattt gctcaattaa gctggtattt ccgtcgggaa tggcgtcgct
    24121 atctcggggc tgtcgccttg cttgtcatta tcgcgatgct gcaactggtt ccgccaaaag
    24181 tggttggtat tgttgtcgat ggcgtgacag aacaacactt tactaccggg cagatcctga
    24241 tgtggatcgc caccatggtg ctgattgccg ttgtggttta tctcctgcgt tacgtctggc
    24301 gggtattgct gtttggtgcg tcttatcaac tggctgttga actgcgtgaa gattattacc
    24361 gtcagctaag ccggcagcat cctgagtttt acctgcgtca tcgcacgggt gatctcatgg
    24421 ctcgtgcgac caatgacgtc gatcgcgtcg tgtttgccgc cggagaaggg gtgctgacgc
    24481 tggtggattc actggtgatg ggctgcgctg tgttgattat gatgtctacg caaattagct
    24541 ggcagttgac cttatttgcc ctgttgccga tgccagtgat ggcgatcatg atcaagcgca
    24601 acggcgatgc cttgcatgaa cgctttaagc tggcacaggc ggcgttttct agtcttaatg
    24661 accgcaccca ggaaagcctc accagtatcc gcatgatcaa agcctttggt ctggaagatc
    24721 gccagtcggc gttatttgcc gcggatgccg aagataccgg caaaaaaaac atgcgggtgg
    24781 cgcgtattga tgctcgtttc gacccaacca tctatatcgc gattggtatg gcgaacttgc
    24841 tggcgattgg cggcggtagt tggatggtgg cgcagggcag tttaacgctg ggccagctca
    24901 ccagttttat gatgtattta ggtctgatga tttggccaat gctggcgctg gcatggatgt
    24961 ttaacattgt ggaacgtggt agtgctgcat acagccgtat tcgcgcgatg ctggcggaag
    25021 cgccggtggt gaacgatggt agcgaacccg tgccggaagg gcgtggcgaa ctggatgtaa
    25081 atattcacca gttcacgtat ccgcagactg accatcctgc gctggaaaac gtcaatttcg
    25141 ccctgaaacc cggtcagatg ctgggtatct gcgggccgac tggttccggc aaaagtaccc
    25201 tgttgtcgct cattcagcgt catttcgacg tcagcgaggg ggatattcgc tttcatgata
    25261 ttcctctgac gaagttacaa ctcgatagct ggcgtagccg cctggcggta gttagccaga
    25321 caccgttcct tttttccgac accgtagcga acaacatcgc gttgggttgc ccgaatgcca
    25381 cccagcaaga gattgagcat gtcgcgcggt tagccagcgt acatgacgat attttgcgtt
    25441 taccgcaagg ttacgataca gaggtgggcg agcgcggcgt gatgctttcc ggcgggcaaa
    25501 aacagcgtat ctctattgct cgtgcgttat tagtcaacgc ggaaatcctc atccttgatg
    25561 atgcgctttc ggcggtggac ggacgcacag agcaccagat cctgcataac ctgcgtcagt
    25621 ggggacaggg tagaacggta atcatcagtg cccatcgcct ttcggcactg acggaagcca
    25681 gtgaaattat tgtgatgcag cacggacata tcgcccagcg tggcaatcat gatgagctgg
    25741 cgcaacaaag cggctggtat cgcgatatgt atcgctatca acaactggag gcggcgctcg
    25801 acgacgctcc ggaaattcgc gaggaggccg tcgatgcgta gttttagcca actgtggccg
    25861 actctcaagc gtctgttagc gtacggttcg ccgtggcgta aaccgctggg gattgcggtc
    25921 ctgatgatgt gggttgcggc agcggcagaa gtcagtgggc cgctgcttat cagctatttt
    25981 atcgacaata tggtagcgaa aaataacctg ccgttgaaaa tggttgcagg gctggctgcg
    26041 gcgtatgttg ggctacaact gtttgccgcc gggctacatt acgcgcagtc gctgctgttt
    26101 aatcgggcgg cagtaggcgt agtgcaacag ttgcgtaccg acgtgatgga tgccgcattg
    26161 cgccagccat taagcgagtt tgatacccaa cccgtcgggc aggtgatttc ccgcgtcact
    26221 aatgacactg aagtgatccg cgatctctac gttaccgtag tggcaactgt cctgcgtagc
    26281 gccgcactgg tgggcgcgat gctggtggcg atgttcagcc tcgactggcg aatggcgctg
    26341 gtggcgataa tgattttccc ggtggtgctg gtggtaatgg tgatatacca gcgttacagc
    26401 acgccgattg tccgccgtgt gcgcgcctat ctagcggata tcaacgacgg ctttaacgaa
    26461 atcatcaatg gcatgagcgt tatccagcag ttccgtcagc aggcgcgatt tggcgaacgt
    26521 atgggggagg ccagtcgttc acactatatg gccaggatgc aaaccctgcg cctcgacggt
    26581 tttctgctgc gtccgctgct gagtctgttt tcatcgctca ttctttgtgg cttgttgatg
    26641 ctgtttggct tctccgctag cggcaccatt gaagtgggcg tgctgtatgc gtttatcagc
    26701 tatcttgggc gacttaacga accattaatc gaactgacca cgcaacaggc gatgctgcaa
    26761 caggctgttg ttgctggtga gcgcgtgttt gaactgatgg acggaccgcg ccagcaatat
    26821 ggcaatgatg agcgcccgtt acagagtggc accatcgaag tcgataacgt gtcatttgct
    26881 tatcgcgatg acaatctggt gctaaagaac attaatctct ctgtgccttc gcgtaacttt
    26941 gtggcactgg tcgggcatac cggcagtggc aaaagcaccc tcgccagttt attgatgggc
    27001 tattacccgc taacggaagg tgagattcgc cttgatggtc gtccattaag ttcgctaagt
    27061 cacagcgcgc tgcgccaggg cgtggcaatg gtgcagcaag atccggtggt gctggcggat
    27121 accttcctcg ccaacgtgac gctggggcgt gatatctccg aagaacgcgt ctggcaggcg
    27181 ctggaaaccg tgcaactggc ggagctggcg cgcagcatga gtgatggtat ttatacgccg
    27241 ctgggcgagc aggggaataa tctctccgtc gggcaaaagc aactgctggc actggcacgc
    27301 gtgttggtgg agacgccgca aatcctgatc cttgatgagg caaccgccag cattgactcc
    27361 ggtactgaac aggcgattca acatgctctg gcggctgtgc gtgaacatac cacgctggtg
    27421 gtgattgctc accgcttatc gaccattgtt gatgccgaca ccattctggt gcttcatcgt
    27481 gggcaagccg tggagcaggg cactcaccag caactgctgg cggcccaggg acgctactgg
    27541 cagatgtatc aactgcaact tgcgggcgaa gagctggcag ccagcgtgcg tgaagaagaa
    27601 tcattgagcg cctgaatagc gcaatatttc atcgttggtg caaaaatgta acgcactgtg
    27661 cactgtcata gtgcgttttc attttcaatc ttcttaactt cctgctctct ttctcgtttt
    27721 tcatttctgg cacaccgctt gcaatacctt cttcgtgtag cagaaccatt accgaattct
    27781 gaccggaggg gatctatgaa gctggtgacc gtgataatca aaccattcaa gctggaagac
    27841 gttcgtgaag cgttatcttc cattggtatt cagggcctga ccgtcaccga agtgaaaggt
    27901 ttcgggcgtc agaaagggca tgccgagctg taccgggggg cggaatacag cgtcaacttc
    27961 ctgccaaaag taaaaattga tgtggcaatt gctgatgacc aactcgatga agtgatcgat
    28021 atcgtcagta aggcggctta caccggaaaa attggcgacg gcaaaatctt cgtcgctgaa
    28081 ttgcaacgcg tcattcgtat tcgtaccggc gaagccgacg aagcggcgct gtaatctctg
    28141 gcacacagca acaggaacga aaaatgaaga tagcgacgat aaaaactggg cttgcttcac
    28201 tggcgatgct gccgggactg gtaatggctg cacctgcggt ggccgataaa gccgacaatg
    28261 cgtttatgat gatttgtact gctctggtgc tgtttatgac tattccgggg attgccctgt
    28321 tttacggtgg gttgattcgc ggcaaaaacg ttctgtcgat gctgacgcag gtgacggtga
    28381 cattcgcact ggtctgtatt ctctgggtgg tttacggtta ctcgctggcg tttggtgagg
    28441 gcaacaactt cttcggcaac attaactggc tgatgctgaa aaacatcgaa ctgacggcgg
    28501 tgatgggcag catttatcag tatatccacg tagcgtttca gggatcgttt gcctgcatta
    28561 ccgtaggctt gatcgttggg gcgctggcgg aacgaatccg cttctcagct gtgttgattt
    28621 tcgtggtggt atggctgacg ctctcttaca ttccaattgc gcatatggtc tggggcggtg
    28681 gtttgctggc ttctcacggt gcgctggatt tcgcgggtgg cacggtggtg cacattaacg
    28741 ccgcaatcgc cggtctggtg ggcgcgtatc tgataggaaa acgcgtgggc ttcggtaaag
    28801 aggcgtttaa accacacaac ctgccgatgg tcttcaccgg gactgccatt ctctatatcg
    28861 gttggtttgg ctttaacgcc gggtcagcgg gcacggcgaa tgaaatcgcg gcactggcat
    28921 ttgtgaatac tgtggtcgca acggcggcgg caattcttgg ctggatcttc ggtgaatggg
    28981 cgctgcgtgg taagccttct ctgctgggag cgtgttctgg cgcgattgcc ggtctggtcg
    29041 gcgtgacgcc agcgtgtggc tacattgggg ttggcggcgc gttgattatc ggcgtggtag
    29101 ctggtctggc gggcttgtgg ggcgttacta tgctcaaacg cttgctgcgg gtggatgatc
    29161 cctgcgatgt cttcggtgtg cacggcgttt gtggcattgt cggctgtatc atgactggga
    29221 tttttgccgc cagttcgctg ggtggcgtgg gcttcgctga aggtgtgacg atgggccatc
    29281 agttgctggt acagctggaa agcatcgcca ttacgatcgt ctggtccggt gtggtggcat
    29341 ttattggcta caaactggcg gatctgacgg ttggtctgcg tgtaccggaa gagcaggagc
    29401 gagaagggct ggatgtcaac agtcacggcg agaatgccta taacgcgtaa caagcactgc
    29461 aaaaaatagc cggacggttt tacctccggc tattttttta attgtgatta cgcatcaccc
    29521 cttcctgaac ggtcgaggca accagcacgc cgtcctgggt ataaaactca ccgcgcacaa
    29581 agccgcgtgc gctggacgcc gaggtgctct ccacgctata cagcagccat tcattcaaat
    29641 taaacgggcg atggaaccac atggaatggt caatggtggc aatctgaatc cccggttcga
    29701 gaaaaccgat gccgtgcggc tgtagagcta ccggcaggaa gttaagatca gaagcgtaac
    29761 cgagcagata ctgatgaacg cgcaggtcat ccggcacgct accatttgcg cggatccaca
    29821 cctgacgatg tggttctgcg acgtgacctt tcagtgggtt atgaaactcc accggacgga
    29881 cttccagcgg acgatcgcag atgaatttat ctttaagcac tggcggcagc aggtgcgcca
    29941 gcgattgggc gatttgcgtt tccgaaggca ggccatcagg cgctggcgcg gacggcattg
    30001 ttttttgatg ttcgaaaccc gcttctggtg cctggaaaga ggccgtcata taaaaaatcg
    30061 gtttgccgtt ttgaatagca gcaacccggc gggcgctgaa gctgttaccg tcacgcagcg
    30121 tttcgacatc ataaataatc ggcttcttac tatcgccagg gcgaagaaag tagctgtgaa
    30181 acgaatgtac cagccgctct tcagggacgg tctcttttgc agcatacaag gcctgaccca
    30241 cgacctggcc gccaaacacc tggcgtaaac ctaaatcttc actctggccg cgaaagagtc
    30301 cttcctcaat tttttccaga tttaacaatg tcagtaaatt ttttagcgcc tgactcatat
    30361 aactctccag taacaaagct gccgcagcaa gccaaagtga gttgagtata acgcaaattt
    30421 gctactggtc cgatgggtgc aatggtctga attacgggct aattacaggc agaaatgcgt
    30481 gatgtgtgcc acacttgttg acgttactat tttgttaacc actcttccgg cgaggaaagt
    30541 tagcccgctg gtgcattgat aataaggaga aatgaatgaa actcgtgcac atggccagtg
    30601 gtttagcggt tgcgattgcg ttggcggctt gcgcagataa aagcgcggat attcagacgc
    30661 cagccccggc tgcaaatacg tctatttcag caacacaaca acctgctatc cagcaaccga
    30721 atgtctccgg taccgtctgg atccgtcaga aagtcgcact gccgcctgat gctgtgctga
    30781 ccgtgacact ttctgacgcg tcgttagccg atgcaccgtc aaaagtgctg gcgcagaaag
    30841 cggtgcgtac tgaaggtaaa cagtcaccat tcagctttgt tctgccattt aacccggcag
    30901 atgttcagcc gaacgcgcgt attctgttga gtgcggcgat taccgtgaat gacaaactgg
    30961 tatttattac cgataccgtt cagccggtga tcaacaaggg cggaactaaa gccgacctga
    31021 cattggtgcc ggtgcagcaa accgccgtgc cggttcaggc cagcggtggt gcaacgacta
    31081 ccgtaccttc gacttcacca actcaggtga atccgtcttc ggcagttcca gctcctacgc
    31141 aatattaagc gacgttaacc ctctccgccc ggagagggtt agtagttcca gcgataacgc
    31201 tgcaagtcga tttgcccgct tcccgatacc atcacacctt ctgccagtaa tgcctgtcgc
    31261 tgacgctgta aatccggtcc ggttagcgaa attgtgccgt ggcgattaac cacccggtgc
    31321 cagggtaagg tgctgccttc agggagacgc tttaacacac cgcccacctg gcgcgcggcg
    31381 cggggcgatc ccgccagttt cgccacatca ccgtaagtgg tgacatagcc ttcgggaata
    31441 gcggcgacga tttgccagac gcgttgggga aatgaatctt ctttttccat cttttcttcc
    31501 tgaggtaatt tttcagcata atctggaaaa acgcccgagt gaagtcgcat tgcgcaagaa
    31561 accagcatct ggcacgcgat gggttgcaat tagccggggc agcagtgata atgcgcctgc
    31621 gcgttggttc tcaacgctct caatgggggc tctgttggtt ctcccgcaac gctactctgt
    31681 ttaccaggtc aggtccggaa ggaagcagcc aaggcagatg acgcgtgtgc cgggatgtag
    31741 ctggcagggc ccccacccat ttcagggtaa gtacttgata gataatgtct aaagtactga
    31801 aatctctccc ccatagcctt agtggtacaa aaactgtgta tcaccagtag gctgcacgat
    31861 gtcccttatg ctctcccgtc atgggatctg gtactaccgg aaagttaacg tcttaccctg
    31921 tggtaaacgc cgtgagtttc gtcgttcgtt aggtactcgt tcaaagactg aagcgacaaa
    31981 gcgatcttta cgctttgcag atcgcccttg gctgattact gagacgtcca cgactacaat
    32041 cgatttgatc caatccccta attcagactt gtcgcttagc gagctaaaac tacatgctga
    32101 gcagtacgtt gagtttaaaa gccgtgaagt ttgtgaaagg gaagtttctt ctatcaaacg
    32161 ctgtattgat aagtacttta gcttcactga gcaggtgttt aagcgtagta gggctgctga
    32221 attcattagc gggctggatg tcgcgattcc tacaaagaac aaatatgtta agaaaatcag
    32281 tgggtttttt cgatggctga aacaccgtac tgactatgat tttcaaaatc cctttgaggg
    32341 attgctccaa aaggatcgcg agccagtttc ccaaaagcga gaggcatata cctctgctca
    32401 gatcagatgc ctagagaatg gaatcagtac attacctgac tggaagatat ggattatttt
    32461 gcttggtcga tttacgggga tgagggctaa tgaaatttgt caactatatc atggtgatat
    32521 attaaaaaat gagggaagat ggtgcatcag gattgatgat cgtcatgatg gacaggtggt
    32581 caaaacggag aacagtaaac gtacaatacc aattcataat aaattgatag atattggatt
    32641 tttggactat gtacagaaac agaaaggcag actgtttccg cagttaactt tttataaagg
    32701 gtcatacagc cactacttca cccgctggtt cagcggttac cgcaaaaaat taggggttcc
    32761 agagtttcat tcactgcgac attatattgc cactaagtta aaaaatgctg gtgtagtgga
    32821 gcattatgct gctgcattct taggacatgc agataattca attacataca gaagatatgg
    32881 taagtcagtc gcggtatctc aactatatga tctattagaa catatttgat tcgtactaag
    32941 cagcctcttc gctgagagcc tgcttagtg
//
LOCUS       DAPHDW010000038        32838 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.038, whole genome shotgun sequence.
ACCESSION   DAPHDW010000038 DAPHDW010000000
VERSION     DAPHDW010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32838
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.038"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..273)
                     /locus_tag="RH057_001794"
     CDS             complement(<1..273)
                     /locus_tag="RH057_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3128174.1"
                     /translation="MKIICRLLLAMACLCLANISWATVCANSTGVAEDEHYDLSNIFN
                     STNNQPGQIVVLPEKSGWVGVSAICPPGTLVNYTYRSYVTNFIVQET"
     gene            complement(286..2895)
                     /locus_tag="RH057_001795"
     CDS             complement(286..2895)
                     /locus_tag="RH057_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HDV3128175.1"
                     /translation="MKIPTTTDIPQRYSWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPARVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQITL
                     LSSAHGYIPPEEWDEGIPALLLNYNFTGNRGNGNDSYFFSELSGINIGPWRLRNNGSW
                     NYFRGNGYHSEQWNNIGTWVQRAIIPLKSELVMGDGNTGSDIFDGVGFRGVRLYSSDN
                     MYPDSQQGFAPTVRGIARTAAQLTIRQNGFIIYQSYVSPGAFEITDLHPTSSNGDLDV
                     TIDERDGNQQNYTIPYSTVPILQREGRFKFDLTAGDFRSGNSQQSSPFFFQGTALGGL
                     PQEFTAYGGTQLSANYTAFLLGLGRNLGNWGAVSLDVTHARSQLADDSRHEGDSIRFL
                     YAKSMNTFGTNFQLMGYRYSTQGFYTLDDVAYRRMEGYEYDYDYDGEHRDELIIVNYH
                     NLRFSRKDRLQLNISQSLNDFGSLYISGTHQKYWNTSDSDTWYQVGYTSSWVGISYSL
                     SFSWNESVGIPDNERIVGLNVSVPFNVLTKRRYTRENALDRAYASFNANRNSNGQNSW
                     LAGVGGTLLEGHNLSYHVSQGDTSNNGYTGSATANWQAAYGTLGVGYNYDRDQHDVNW
                     QLSGGVVGHENGITLSQPLGDTNVLIKAPGAGGVRIENQTGILTDWRGYAVMPYATVY
                     RYNRIALDTNTMGNSIDVEKNISSVVPTQGALVRANFDTRIGVRALITVTQGGKPVPF
                     GSLVRENSTGITSMVGDDGQVYLSGAPLSGELLVQWGDGANSRCIAHYVLPKQSLQQA
                     VTVISAVCTHPGS"
     gene            complement(2926..3618)
                     /gene="fimC"
                     /locus_tag="RH057_001796"
     CDS             complement(2926..3618)
                     /gene="fimC"
                     /locus_tag="RH057_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV3128176.1"
                     /translation="MMTKIKLLMLIIFYLIISASTHAAGGIALGATRIIYPADAQQTA
                     VWIRNSHTNERFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVNSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(3838..4380)
                     /gene="fimA"
                     /locus_tag="RH057_001797"
     CDS             complement(3838..4380)
                     /gene="fimA"
                     /locus_tag="RH057_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV3128177.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIASSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            4861..5727
                     /gene="folD"
                     /locus_tag="RH057_001798"
     CDS             4861..5727
                     /gene="folD"
                     /locus_tag="RH057_001798"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HDV3128178.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDTLNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQDE"
     gene            5729..5941
                     /gene="ybcJ"
                     /locus_tag="RH057_001799"
     CDS             5729..5941
                     /gene="ybcJ"
                     /locus_tag="RH057_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HDV3128179.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            6049..6570
                     /locus_tag="RH057_001800"
     CDS             6049..6570
                     /locus_tag="RH057_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3128180.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKVIPPRLLFAGIILAMLPDADVLS
                     FKFGVAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSL
                     LDSVTTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTPYGHQVIISELMWVWLP
                     GMVLMGMLWWRRR"
     gene            complement(6606..7991)
                     /gene="cysS"
                     /locus_tag="RH057_001801"
     CDS             complement(6606..7991)
                     /gene="cysS"
                     /locus_tag="RH057_001801"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HDV3128181.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNGDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNPMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            8165..8659
                     /gene="ppiB"
                     /locus_tag="RH057_001802"
     CDS             8165..8659
                     /gene="ppiB"
                     /locus_tag="RH057_001802"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HDV3128182.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRSGMHQDVPKEDVIIESV
                     TVSE"
     gene            8662..9384
                     /gene="lpxH"
                     /locus_tag="RH057_001803"
     CDS             8662..9384
                     /gene="lpxH"
                     /locus_tag="RH057_001803"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HDV3128183.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQTLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            9502..10011
                     /gene="purE"
                     /locus_tag="RH057_001804"
     CDS             9502..10011
                     /gene="purE"
                     /locus_tag="RH057_001804"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HDV3128184.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAAEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNDWRKAQTDEVL
                     ENPDPRGAA"
     gene            10008..11075
                     /gene="purK"
                     /locus_tag="RH057_001805"
     CDS             10008..11075
                     /gene="purK"
                     /locus_tag="RH057_001805"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HDV3128185.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQN
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFERLGELAIVKRRTGGYDGRGQWRLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NSPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFG"
     gene            complement(11280..12173)
                     /locus_tag="RH057_001806"
     CDS             complement(11280..12173)
                     /locus_tag="RH057_001806"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HDV3128186.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRGKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(12170..12985)
                     /locus_tag="RH057_001807"
     CDS             complement(12170..12985)
                     /locus_tag="RH057_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HDV3128187.1"
                     /translation="MTIIHPLLASRSAPNYRQSWRLAGVWRRAINLMTESGELLTLHR
                     QGSGFGPGGWVLRRAQFDALCGGLCGNERPQVVAQGIRLGRFTVKQPQRYCLLRITPP
                     AHPQPLAAAWMQRAEETGLFGPLALAASDPLPAELRQFRHCFQAALNGVKTDWRHWLG
                     KGPGLTPSHDDTLSGMLLAAWYYGALDARSGRQFFACSDNLQLVTTAVSVSYLRYAAQ
                     GYFASPLLHFVHALSCPKRTAVAIDSLLALGHTSGADTLLGFWLGQQLLQGKP"
     gene            complement(12996..14255)
                     /locus_tag="RH057_001808"
     CDS             complement(12996..14255)
                     /locus_tag="RH057_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009029994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HDV3128188.1"
                     /translation="MFTSVAQANAAVIEQIRRARPHWLDVQPASSLISELNQGKTLLH
                     AGPPMRWQEMTGPMKGACVGACLFEGWAKDEAQALAMLEQGEVNFIPCHHVNAVGPMG
                     GITSASMPMLVVENVTDGNRAYCNLNEGIGKVMRFGAYGEDVLTRHRWMRDVLMPVLS
                     AALGRMEHGIDLTAMMAQGITMGDEFHQRNIASSALLMRALAPQIARLDHDKQHIAEV
                     MDFLSVTDQFFLNLAMAYCKAAMDAGAMIRAGSIVTAMTRNGNMFGIRVSGLGERWFT
                     APVNTPQGLFFTGFSQEQANPDMGDSAITETFGIGGAAMIAAPGVTRFVGAGGMEAAR
                     AVSEEMAEIYLERNMQLQIPGWDFQGACLGLDIRHVVETGITPLINTGIAHKEAGIGQ
                     IGAGTVRAPLACFEQALEALAESMGIG"
     gene            complement(14265..15932)
                     /locus_tag="RH057_001809"
     CDS             complement(14265..15932)
                     /locus_tag="RH057_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HDV3128189.1"
                     /translation="MIHAFIKKGCFQDSVSLMIISRKLSESENVDDVSVMMGTPANKA
                     LLDTTGFWHDDFNNATPNDICVAIRSEAADAGIAQAIMQQLEEALKQLAQGSGSSQSL
                     TQVRRWDSACQKLSDANLALISVAGEYAAELANQALDRNLNVMMFSDNVTLEDEIQLK
                     TRAREKGLLVMGPDCGTSMIAATPLAFANVMPEGNIGVIGASGTGIQELCSQIALAGE
                     GITHAIGLGGRDLSREVGGISALTALEMLSADEKSEVLAFVSKPPAEAVRLKIVNAMK
                     ATGKPTVALFLGYTPAVARDENVWFASSLDEAARLACLLSRVTARRNAITPASSGFIC
                     GLYTGGTLAAEAAGLLAGHLGVEADDTHHHGMMLDADGHQIIDLGDDFYTVGRPHPMI
                     DPALRNQLIADLGAKPQVRVLLLDVVIGFGATADPAASLVSAWQKACAARSDSQPLYA
                     IATVTGTERDPQCRSQQIATLEDAGIAVVSSLPEATLLAAALIHPLSSATQQHTPSLL
                     ENVAVINIGLRSFALELQSASKPVVHYQWSPVAGGNKKLARLLERLQ"
     gene            16249..17298
                     /gene="allD"
                     /locus_tag="RH057_001810"
     CDS             16249..17298
                     /gene="allD"
                     /locus_tag="RH057_001810"
                     /EC_number="1.1.1.154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate dehydrogenase"
                     /protein_id="HDV3128190.1"
                     /translation="MKISRETLHQLIENKLCQAGLKREHAATVAEVLVYADARGIHSH
                     GAVRVEYYAERISKGGTNREPEFRLEETGPCSAILHADNAAGQVAAKMGMEHAIKTAQ
                     QNGVAVVGISRMGHSGAISYFVQQAARAGLIGISMCQSDPMVVPFGGAEIYYGTNPLA
                     FAAPGEGDEILTFDMATTVQAWGKVLDARSRNMSIPDTWAVDKNGAPTTDPFAVHALL
                     PAAGPKGYGLMMMIDVLSGVLLGLPFGRQVSSMYDDLHAGRNLGQLHIVINPNFFSSS
                     ELFRQHLSQTMRELNAITPAPGFNQVYYPGQDQDIKQRKAAVEGIEIVDDIYQYLISD
                     ALYNTSYETKNPFAQ"
     gene            17320..18555
                     /gene="allC"
                     /locus_tag="RH057_001811"
     CDS             17320..18555
                     /gene="allC"
                     /locus_tag="RH057_001811"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HDV3128191.1"
                     /translation="MITHFRQAIEETLPWLSSFGADPAGGMTRLLYSPEWLETQQQFK
                     KRMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWL
                     AIDWLKTQYSAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNS
                     FVDAMKACGFTLPNAPLTPRQDIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTV
                     TLNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNV
                     VPGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKEL
                     VATLTELCESEKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITD
                     LAEGVKTLALMLYQLAWQK"
     gene            18566..19351
                     /gene="allE"
                     /locus_tag="RH057_001812"
     CDS             18566..19351
                     /gene="allE"
                     /locus_tag="RH057_001812"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HDV3128192.1"
                     /translation="MGYLNNVTGYRDDLLANRAIVKHGNFALLTPDGLVKNIIPGFEN
                     CDATILSTPKLGASFVDYLVTLHQNGSNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLVSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVYNLDNN
                     WIPVKKGDYIFMGAYSLQAGYGVGRGEAFSYIYSKDCNRDVEI"
     gene            complement(19480..20625)
                     /gene="glxK"
                     /locus_tag="RH057_001813"
     CDS             complement(19480..20625)
                     /gene="glxK"
                     /locus_tag="RH057_001813"
                     /EC_number="2.7.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 3-kinase"
                     /protein_id="HDV3128193.1"
                     /translation="MKIVIAPDSFKESLSAEKCCQAIKAGFSTLFPDANYICLPIADG
                     GEGTVDAMVAATGGNIVTLEVCGPMGEKVNAFYGLTGDGKTAVIEMAAASGLMLVAPE
                     KRNPLLASSFGTGELIRHALDNGIRHIILGIGGSATVDGGMGMAQALGVRFLDADGQV
                     LVANGGNLARVASIEMDECDPRLANCHIEVACDVDNPLVGARGAAAVFGPQKGATPEM
                     VEELEQGLQNYARVLQQQTEINVCQMAGGGAAGGMGIAAAVFLNADIKPGIEIVLNAV
                     NLAQAVQGAALVITGEGRIDSQTAGGKAPLGVASVAKQFNVPVIGIAGVLGDGVEVVH
                     QYGIDAVFSILPRLAPLAEVLASGETNLFNSARNIACAIKIGQGIKN"
     gene            complement(20647..21948)
                     /locus_tag="RH057_001814"
     CDS             complement(20647..21948)
                     /locus_tag="RH057_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308602.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil/xanthine transporter"
                     /protein_id="HDV3128194.1"
                     /translation="MFNFAVSRESLLSGFQWFFFIFCNTVVVPPTLLSAFQLPQSSLL
                     TLTQYAFLATALACFAQAFCGHRRAIMEGPGGQWWGTILTITLGEASRGTPINDIATS
                     LAVGIALSGVLTMLIGFSGLGHRLARLFTPSVMVLFMLMLGAQLTTIFFKGMLGLPFG
                     IADPNFKIQLPPFALSVAVMCLVLGMIIFLPQRFARYGLLVGTITGWLLWYFCFPSSH
                     SLSGELHWQWFPLGSGGALSPGIILTAVITGLVNISNTYGAIRGTDVFYPQQGAGNTR
                     YRRSFVATGFMTLITVPLAVIPFSPFVSSIGLLTQTGDYTRRSFIYGSVICLLVALVP
                     ALTRLFCSIPLPVSSAVMLVSYLPLLFSALVFSQQITFTARNIYRLALPLFVGIFLMA
                     LPPVYLQDLPLTLRPLLSNGLLVGILLAVLMDNLIPWERIE"
     gene            complement(22005..23366)
                     /gene="allB"
                     /locus_tag="RH057_001815"
     CDS             complement(22005..23366)
                     /gene="allB"
                     /locus_tag="RH057_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase AllB"
                     /protein_id="HDV3128195.1"
                     /translation="MSFDLIIKNGTVILENEARVVDIAVKGGKIAAIGQDLGDAKEVM
                     DASGLVVSPGMVDAHTHISEPGRSHWEGYETGTRAAAKGGITTMIEMPLNQLPATVDR
                     ASIELKFDAAKGKLTIDAAQLGGLVSYNIDRLHELDEVGVVGFKCFVATCGDRGIDND
                     FRDVNDWQFFKGAQKLGELGQPVLVHCENALICDALGEEAKREGRVTAHDYVASRPVF
                     TEVEAIRRVLYLAKVAGCRLHVCHISSPEGVEEVTRARQEGQDVTCESCPHYFVLDTD
                     QFEEIGTLAKCSPPIRDLENQKGMWEKLFNGEIDCLVSDHSPCPPEMKAGNIMEAWGG
                     IAGLQNCMDVMFDEAVQKRGMSLPMFGKLMATNAADIFGLQQKGRIAPGKDADFVFIQ
                     PNSSYVLTNDDLEYRHKVSPYVGRTIGARITKTILRGDVIYDIEQGFPVAPKGQFILK
                     HQQ"
     gene            complement(23426..24880)
                     /locus_tag="RH057_001816"
     CDS             complement(23426..24880)
                     /locus_tag="RH057_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative allantoin permease"
                     /protein_id="HDV3128196.1"
                     /translation="MEHQRKLFQQRGYSEDLLPKTQSQRTWKTFNYFTLWMGSVHNVP
                     NYVMVGGFFILGLSTFSIMLAIILSAFFIAAVMVLNGAAGSKYGVPFAMILRASYGVR
                     GALFPGLLRGGIAAIMWFGLQCYAGSLACLILIGKIWPGFLTLGGDFTLLGLSLPGLI
                     TFLLFWLVNVGIGFGGGKVLNKFTAILNPCIYIVFGGMAIWAISLVGIGPIFDYIPSG
                     IQKAENSGFLFLVVINAVVAVWAAPAVSASDFTQNAHSFREQALGQTLGLVVAYILFA
                     VAGVCIIAGASIHYGADTWNVLDIVQRWDSLFASFFAVLVILMTTISTNATGNIIPAG
                     YQIAAIAPTKLTYKNGVLIASIISLLICPWKLMENQDSIYLFLDIIGGMLGPVIGVMM
                     AHYFVVMRGQINLDELYTAPGDYKYYDNGFNLTAFSVTLVAVILSLGGKFIPFMEPLS
                     RVSWFVGVIVAFAAYALLKKRTTAEKTGEQKTIG"
     gene            complement(25050..25928)
                     /gene="glxR"
                     /locus_tag="RH057_001817"
     CDS             complement(25050..25928)
                     /gene="glxR"
                     /locus_tag="RH057_001817"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV3128197.1"
                     /translation="MKLGFIGLGIMGTPMAVNLARAGHQLHVTTIGPVAEELLALGAV
                     NVETARQVTESSDIIFIMVPDTPQVEEVLFGENGCAKTSLKGKTIVDMSSISPIETKR
                     FARQVNELGGDYLDAPVSGGEIGAREGTLSIMVGGDEAVFERVKPLFELLGKNITLVG
                     GNGDGQTCKVANQIIVALNIEAVSEALLFASKAGADPVRVRQALMGGFASSRILEVHG
                     ERMIKRTFNPGFKIALHQKDLNLALQSAKALALNLPNTATCQELFNTCAANGGSQLDH
                     SALVQALELMANHKLA"
     gene            complement(26027..26803)
                     /gene="hyi"
                     /locus_tag="RH057_001818"
     CDS             complement(26027..26803)
                     /gene="hyi"
                     /locus_tag="RH057_001818"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HDV3128198.1"
                     /translation="MLRFSANLSMLFQEYDFLERFDKAAQCGFRAVEFMFPYDYDIEV
                     LQHKLSSNNLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAVAIRYARALGNKKI
                     NCLVGKTPVGVSAEQVHTTLVENLRYAANMLAKEGILLLIEPLNHFDMPGFHLTGTQQ
                     ALALIADIGSNNIKIQYDIYHMQRMEGELAQTMTTWANKIGHLQIADNPRRGEPGTGE
                     INYDFLFDLIEKSDYDGWVGCEYKPLTTTEAGLSWINPYR"
     gene            complement(26816..28597)
                     /gene="gcl"
                     /locus_tag="RH057_001819"
     CDS             complement(26816..28597)
                     /gene="gcl"
                     /locus_tag="RH057_001819"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HDV3128199.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGASHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGVCAADPERKVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMNQYRVPVVVEIILERVTNISMGNE
                     LDNVMEFEDIADNAADAPTETCFMQYK"
     gene            complement(28687..29502)
                     /gene="allR"
                     /locus_tag="RH057_001820"
     CDS             complement(28687..29502)
                     /gene="allR"
                     /locus_tag="RH057_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HDV3128200.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGSVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            complement(29580..30062)
                     /gene="allA"
                     /locus_tag="RH057_001821"
     CDS             complement(29580..30062)
                     /gene="allA"
                     /locus_tag="RH057_001821"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HDV3128201.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIYELERHPLGTQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            30292..31218
                     /gene="allS"
                     /locus_tag="RH057_001822"
     CDS             30292..31218
                     /gene="allS"
                     /locus_tag="RH057_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HDV3128202.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YHPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSARTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSGKAVEDIVTLFTQRRPEISGFLEIFGNPRS"
     gene            31287..32381
                     /gene="mnmH"
                     /locus_tag="RH057_001823"
     CDS             31287..32381
                     /gene="mnmH"
                     /locus_tag="RH057_001823"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HDV3128203.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMN
                     NDERAAVGTCYKQQGSDAALALGHKLVAGEIRQQRMDAWRAACLQNPQGILCCARGGQ
                     RSHIVQSWLYAAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAEWVKAR"
     gene            32497..32838
                     /locus_tag="RH057_001824"
                     /pseudo
     CDS             32497..32838
                     /locus_tag="RH057_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014543172.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT         8229 a         8748 c         8056 g         7805 t
ORIGIN      
        1 agtttcctga acaataaagt tggtgacata actacggtat gtataattca ccagcgtgcc
       61 gggtggacaa attgctgaca cacctaccca gccggatttt tccggtaaaa caacaatctg
      121 ccctggctgg ttattggtgc tattaaagat atttgagaga tcatagtgtt catcttctgc
      181 tacgccagta ctatttgcac aaacagtagc ccaggatatg tttgccagac acaaacatgc
      241 catcgccaat aataatctgc agattatctt cattgataat ttcctttatg agccaggatg
      301 tgtgcaaact gccgaaataa cagtgacggc ttgctgtaag ctttgcttcg gcaatacata
      361 gtgcgcaatg cagcgtgagt tcgcgccgtc tccccactga accagtaatt caccagacaa
      421 tggcgcacca cttaaataaa cttgcccgtc atcacccacc atactggtta ttccggtact
      481 gttttcccgt accagtgatc caaacggcac cggttttccg ccctgggtaa cggtaatgag
      541 cgcccgcacg cctatgcggg tatcaaaatt ggcacgaacc aacgcgcctt gcgtcggcac
      601 aacgctgctg atattttttt caacatcgat ggaattcccc atcgtattgg tatcaagcgc
      661 gatacggtta taccgataaa ccgtggcata cggcatcacc gcatagccgc gccagtcggt
      721 taaaatgcca gtttgatttt caatgcgtac accgcctgcg ccaggcgctt taatcaaaac
      781 attggtatcc cctaaaggct ggctcagcgt tatgccattt tcgtgcccga ccacaccgcc
      841 agacagctgc cagttaacgt catgttgatc gcggtcgtag ttatacccga cccccagcgt
      901 accgtaagcg gcctgccagt ttgccgtggc gctgcccgta tacccattat tcgaggtatc
      961 accctggctt acgtgataac tcaggttgtg gccttctagt aaggtcccac ctacacctgc
     1021 cagccagcta ttttgcccgt tgctgttacg gttggcgtta aaggaggcat aagcgcggtc
     1081 gagcgcattt tcccgggtgt agcgacgttt ggtcagaaca ttgaaaggca ctgaaacatt
     1141 aagtccgaca atacgttcgt tatcggggat ccctacagat tcattccacg aaaatgagag
     1201 cgaataactg atgccaaccc agctgctggt ataccccacc tgataccagg tatctgaatc
     1261 cgaagtattc cagtattttt gatgggtacc agagatataa agcgagccaa agtcattaag
     1321 tgattgtgaa atatttaact gcaaacggtc tttacggcta aagcgtaaat tgtggtaatt
     1381 cacgattatc agttcatccc gatgctcacc gtcgtaatcg taatcatatt cgtacccctc
     1441 cattcgacga tacgcaacat catcaagggt ataaaaacct tgtgtcgaat agcggtaacc
     1501 cattaactga aaattggtgc cgaaggtgtt catcgatttc gcatagagga agcgaatgga
     1561 atccccctca tgacgactgt cgtcggctaa ctgactgcgc gcatgggtca catccagcga
     1621 cactgccccc cagtttccga gattgcgccc cagtcctaat aaaaaggcgg tgtaattggc
     1681 agataattgc gtcccgccgt aggcagtaaa ttcctgtggt aaaccgccga gtgccgtgcc
     1741 ctgaaaaaag aaaggtgatg attgctgact attaccgcta cgaaaatcgc ccgccgtcag
     1801 gtcaaatttg aaacgccctt cgcgttgtaa aatcggcact gttgaatacg gaattgtgta
     1861 attctgctga ttgccatcgc gctcgtcgat ggtgacatcc agatcgccat ttgaagatgt
     1921 cgggtgcaaa tctgtaattt caaaagcgcc gggggaaaca tagctttgat agataataaa
     1981 accattttgc cgaatcgtta gctgggccgc cgtacgggca atcccacgta ccgttggggc
     2041 aaacccttgc tggctatcag gatacatatt atcggaagaa taaagccgta caccacgaaa
     2101 tccaacacca tcgaaaatat cacttcctgt attgccgtct cccattacca gttcactttt
     2161 cagcggaata atggcgcgct gtacccaggt gccaatatta ttccactgtt ctgaatgata
     2221 tccatttccg cgaaaatagt tccaggaacc attgttgcgt aaacgccacg ggccaatatt
     2281 aatcccgctg agctcactaa aaaaatagct atcgttacca ttacctctgt taccggtgaa
     2341 attataattc aggagtaaag caggaatacc ttcatcccac tcttcaggcg gaatgtaacc
     2401 gtgagcgcta ctcaacaacg ttatctgagg aatagtgatg ttcaggcgca tcgctgcaaa
     2461 atcaaagtta attgtggcat caggtacagc tttcagtaaa ttgatgcatt tattgttttg
     2521 ctgctgggct aactcgggaa atgcactgct attaaggcca attcgttcaa ggagtacctg
     2581 gttaaaacag ggcattaacc cacctgattt atcacctgta ttttctgttg tcgattcaaa
     2641 tacgatatcc tgtgaaccaa tgaactcatc attacgccag agatccaccc gatacacgcg
     2701 cgcaggttga tgattacctc tctcaaagcg cgataaatca gcaacggatg tgccattctc
     2761 taataaaaat gccgggttga aataactttc cgcatagctt aaaaaggagg gtaaaatggc
     2821 aagagatgaa taacaaattc cggccagaca ccagctatac ctctgcggaa tatccgtagt
     2881 agtgggtatt ttcataatta gctctgatat tgtcagatga ttcggttagt ttaagttcac
     2941 ttctctgacc ggggttactg aaccataatc attaacggtc tgaaatttca gctttccctg
     3001 cactcctgag ggtaagggaa tttgtgaatt aactctgggt gcagccatag cattccccaa
     3061 cttttggctg cccacttgta agttaaccag ggtgacataa aaaggtgagg gattatgaac
     3121 attgatatag ttaccggatc gcgaaaactt gagtgtgtcc ggcgcttctg cgggtaattc
     3181 ttgtaattga attggacgga gaaataattt catgcgcgat aaaatcgcca gttgcaaaac
     3241 attcctgccg ttcaatgcat ttttatctac cgaagggatc gtcttaacat tcatccagaa
     3301 cagagactca cgatctgccg ccagcggtgg accggtgtaa ataatacgca aggtattttc
     3361 gcttttgggt tcactaacaa acagtggcgg tgtaatgatg aatgactttt cttttacacc
     3421 gctgctgttt tcaatccacg aattgaccag aaagcgctca ttggtatggc tatttctaat
     3481 ccataccgca gtctgttgag catcagcggg ataaataata cgcgtggcac ctaatgcgat
     3541 ccctccggca gcatgggtgc tggccgaaat gattaaataa aatataatga gcatcaataa
     3601 ctttatttta gtcatcatag ttaatacgcc ttgtgtattc ctgacggcaa aaataagaaa
     3661 tataaagaaa tgattgcttt ggtttcttac aacacatcat tcccttttca tttaataaat
     3721 cggcatgata caaagtgaac atgatttcga tttacattgc caccataccg cttcagacaa
     3781 ccggtacatt actttgttat aagacgtgat atataattca aaacaacgtg gttttgatta
     3841 ttcgtatctc ataataaaag tcgcgtcagc atttgcttgc cctgctgatg cacttgtacc
     3901 ggtgccttta taacgtgcag aaaaatgaag aacgttggtc ccggggatca agttctggtt
     3961 ggttgagaag ctattcccat caggtttgag aattgcggat gtattatcaa gtatttcaat
     4021 accgacaccc gttgctgttg ttgtattggt actggatgca atggccaata aattatcatt
     4081 gattgcatca gcctgaccag aaaatgcaac ggcagcatta gcggcaacaa caggatcgca
     4141 gtcgttcaac tgaatggtga atggaattaa tgcagaggta ttaccaacag cattgaaaat
     4201 atcggtacga tattgaccga gtgtgacaac ctggtctgcc gagtcagtat tcactgaaca
     4261 ggcagcattc accagttcgc cttcaaagtg aattgtaccg ccatctacaa cggcagcata
     4321 actacccgta gcggcgaata gtgcggcagc cagcgcagac gaaataaatc ttaatttcat
     4381 atatattcct tcaatctcat ttatcgactc cacatccgta tataaccgat tactttattt
     4441 aagacactga tagtagtaaa attccttttt atcctctaag aatgtcttaa ttgaaaatat
     4501 gcactctaat ctaaaaaata gagagccccg ttagatgaat acttccgcgc aaaatatatt
     4561 caacacaaat atagacctga agcggtaaat caccaggctg aaaattcttt ttatattgtc
     4621 aggtatttct taaattatct taatccttaa acaaggaaat aaatcagttc cagatttaca
     4681 acgccatcat ggacgaaaaa tgaagctttc aatctcagcg acctcagcga cggtgcgcct
     4741 aaccttcgca agaggtcgct tcacgcgata aatctgaaac gaaacctgac agcgcgcccc
     4801 gcttctgaca aaataggcgc atccccttcg acctacgtaa cagatggaat cctctctctg
     4861 atggcagcaa agattattga cggtaaaacg attgcgcagc aggtgcgctc tgaagttgct
     4921 caaaaagttc aggcgcgtat tgcagccgga ctgcgggcac caggactggc cgttgtgctg
     4981 gtgggtagta accctgcatc gcaaatttat gtcgcaagca aacgcaaggc ttgtgaagaa
     5041 gtcgggttcg tctcccgctc ttatgacctc ccggaaacca ccagcgaagc ggagctgctg
     5101 gagcttatcg atacgctgaa tgccgacaac accatcgatg gcattctggt tcaactgccg
     5161 ttaccggcgg gtattgataa cgtcaaagtg ctggaacgta ttcatccgga caaagacgtg
     5221 gacggtttcc atccttacaa cgtcggtcgt ctgtgccagc gcgcgccgcg tctgcgtccc
     5281 tgcaccccgc gcggtatcgt cacgctgctt gagcgttaca acattgatac cttcggcctc
     5341 aacgccgtgg tgattggcgc atcgaatatc gtcggtcgcc caatgagcat ggaactgttg
     5401 ctcgcaggtt gcaccactac cgtgactcac cgcttcacta aaaatctgcg tcatcacgta
     5461 gaaaatgccg atctgttgat cgttgccgtt ggcaagccag gctttattcc cggtgactgg
     5521 atcaaagaag gcgcaattgt gattgatgtc ggcatcaacc gtctggaaaa tggcaaagtt
     5581 gtgggcgacg tcgtgtttga agacgcggct aaacgcgcct catacattac gcctgttccc
     5641 ggcggcgttg gcccgatgac ggttgccacg ctgattgaaa acacgctaca ggcgtgcgtt
     5701 gaatatcatg atccacagga tgagtaacat ggcgacattt tctttaggta aacatccgca
     5761 cgttgagctg tgcgacttgc tgaaactgga aggctggagc gaaagcggcg cgcaggcgaa
     5821 aatcgcgatt gccgaaggcc aggtgaaagt cgacggtgcg gttgaaacgc gcaaacgctg
     5881 caaaatcgtc gccggtcaga cagtgagttt tgcaggtcac agcgtacagg ttgttgcctg
     5941 atccctttcc cggcctgtat caacaggccg gttctctttt cagacttttg ccctctacgc
     6001 cccgttcatc catgctgata gcatcgacat tttgccgaag cgaccatcat gccaaccgtt
     6061 attacgcacg ccgccgttcc cctatgcatt ggtttagggt tgggttcgaa agtcatccct
     6121 ccgcgtctgt tatttgccgg aatcatcctg gcgatgctgc ccgacgccga cgtattgtcg
     6181 tttaaatttg gcgttgctta cggcaatgtt tttggtcatc gcgggtttac ccattcgctg
     6241 gtgtttgcgt ttgttgtccc gctgttatgt gtgctgattg gacgccgatg gttcagggct
     6301 gggctgattc gctgctggct gtttttaacc gtctcgttgc tatcgcacag cttgctggat
     6361 tcggtaacca ctggcggtaa gggcgttggc tggctgtggc cgtggtcaga tgaacgcttt
     6421 ttcgctccct ggcaggtgat taaagtcgcg ccgtttgcat tgtctcgtta caccacgccg
     6481 tacgggcatc aggtgataat ttctgaattg atgtgggtgt ggttgccggg gatggtgctt
     6541 atgggaatgt tgtggtggcg caggcgataa ccggatgcga aaactcgcat ccggcaacag
     6601 cgcaattact tacgacgcca ggtggtccct tgcgggccat cttccagcac gatccccatc
     6661 tcgttaagac ggtcacgcgc cgcatctgcc gccgcccagt ctttcgcttt acgggcatcc
     6721 agacgctgtt gaattaacgc ttcaatctca gccacttcgc tgtcgtctgc ctgcgcgccg
     6781 ctttgcagga acgcttccgg ttcttgctcc agcaggccca atacagcgga aagtttacgc
     6841 aggtgagatg ccattgcatt cgccgctgcc atatcttctg ctttcagacg gtttacttca
     6901 cgcgccatat caaacagtac ggaataggct tccggggtgt tgaaatcgtc gtccatcgct
     6961 tcaataaagc gcgcttcaaa cgcttcgcca ccggcaggcg caacggtttt atctgtgccg
     7021 cgcagcgcag tgtagagacg ctccagcgcc gcacgcgctt gcttcaggtt ctcttcgctg
     7081 tagttcaact ggctgcgata gtggcccgac atcaggaagt aacgcacggt ttccgcgtcg
     7141 tagtatttca gcacatcgcg cacggtaaag aagttaccca gcgatttgga catcttctcg
     7201 cggtcaacca tcaccatccc cgagtgcatc cagtagttca catactggcc atcatgggca
     7261 caggtggact gcgcgatttc gttctcgtgg tgcgggaaca tcaggtctga accgccgccg
     7321 tggatatcaa agtggttacc cagctgcttg cagttcattg ccgaacattc aatgtgccag
     7381 cctggacggc ccgcgcccca cggagacggc cagctcggtt cgccctcttt cgacatcttc
     7441 cacagaacga agtccatcgg gttgcgtttg tcatcaacca cgtcaacgcg cgcgccagcc
     7501 tgcagctggt cgagatcctg acgcgacagc acgccataag ttggatcggt cgggacgtcg
     7561 aacatcacgt cgccgttgtc cgccacataa gcgtgacctt tggcgatcag ttgttcagtg
     7621 agttcaataa tttctgcgat atggtgcgtc gcgcgcggct ccatatccgg gcgcagaatg
     7681 ttcaaagcat caaaatcttt gtgcatttcg gcgatcatgc gatccaccag cgccacaaag
     7741 ctttcgccat tttcattggc gcgtttgatg attttgtcgt cgatatcggt aatgttgcgc
     7801 acatacttca gtttatagcc gaggaaacgc agatagcgcg caaccacgtc aaaagcaaca
     7861 aaggtacgcc cgtgaccgat atgacagaga tcgtaaacgg tgattccaca cacgtacatg
     7921 ccgacttccc cggcgtgaat aggcttaaat tcctcttttt ggcgtgtcag agtattgaag
     7981 atttttagca tcgaagattc cgtttagaca tgtgtgggta attgagttgc gtataatacc
     8041 catatttccc gcgcgaatca gcatacattg catgatgatc cgatcgtgcg gttatgctat
     8101 aacaccaccc tatatatgac ccgaactggg ttgaagcacc aatcaaacgg aacaggatgc
     8161 aaaaatggtt actttccaca ccaatcacgg cgatattgtc atcaaaactt ttgacgataa
     8221 agcacctgaa acagttaaaa acttcctgga ctactgccgc gaaggttttt acaacaacac
     8281 cattttccac cgtgttatca acggctttat gattcagggc ggcggttttg aaccgggcat
     8341 gaaacaaaaa gccaccaaag aaccgatcaa aaacgaagcc aacaacggcc tgaaaaatac
     8401 ccgtggtacg ctggcaatgg cacgtactca ggctccgcac tctgcaactg cacagttctt
     8461 catcaacgtg gttgataacg acttcctgaa cttctctggc gaaagcctgc aaggttgggg
     8521 ctactgcgtg tttgctgaag tggttgacgg catggacgtg gtagacaaaa tcaaaggtgt
     8581 tgcaaccggt cgtagcggta tgcaccagga cgtgccaaaa gaagacgtta tcattgaaag
     8641 cgtgaccgtt agcgagtaat cgtggcgaca ctctttattg cagatcttca tctctgcgtg
     8701 gaagaaccgg cgatcaccgc cggttttctg cgttttttag cgggggaagc ccgcaaggcc
     8761 gacgcgctgt atattcttgg cgatctgttt gaagcatgga ttggcgacga cgatcccaac
     8821 ccactccatc gccagatggc ggcagcgatc aaagcggtgt ccgattccgg cgttccctgt
     8881 tatttcattc atggcaaccg tgattttctg ctcggcaaac gctttgcccg tgaaagcggc
     8941 atgacattat tgccggaaga aaaggtgctc gaactttatg gtcgccgggt gttgattatg
     9001 catggcgaca cgctgtgcac cgatgacgcg ggttatcagg cttttcgcgc caaagtccac
     9061 aaaccctggc tgcaaacgct attcctcgcc ctgccgttgt ttgtgcgcaa acgcattgcc
     9121 gcgcgaatgc gagcgaacag taaagaagcc aacagcagta aatcgctggc gatcatggac
     9181 gttaaccaaa acgcggtagt cagtgcgatg gaaaaacatc aggtgcaatg gctgatccac
     9241 gggcataccc atcgcccggc ggtacatgaa cttatcgcca accagcaacc tgcttttcgc
     9301 gtggtactcg gtgcctggca tacagaaggt tcgatggtga aagtcacggc ggatgacgtt
     9361 gagctgattc attttccgtt ttaaaaaacc cgcaactttg ctgatttcac agccacgcaa
     9421 ccgttttcct tgctctcttt ccgtgctatt ctctgtgccc tctaaagccg agagttgtgc
     9481 accacaggag ttttaagacg catgtcttcc cgcaataatc cggcgcgtgt cgccatcgtg
     9541 atggggtcca aaagcgactg ggctaccatg cagttcgccg ccgaaatctt cgaaatcctg
     9601 aatgtcccgc accacgttga agtggtttct gctcaccgca cccccgataa actgttcagc
     9661 ttcgccgaaa gcgccgaaga gaacggttat caggtgatta ttgcgggcgc aggcggcgca
     9721 gcgcacctgc caggcatgat tgccgccaaa acgctggtgc cggtgctggg cgtgccagta
     9781 cagagcgccg cactgagcgg tgtcgatagc ctctactcca tcgtacaaat gccgcgcggc
     9841 attccggtgg gtacgctggc gattggtaaa gctggcgcgg caaacgcggc attactggca
     9901 gcacaaattc ttgcgactca tgataaagaa ctgcaccagc gtctgaatga ctggcgcaaa
     9961 gcccagaccg acgaagtgct ggaaaacccg gacccgcgag gtgcggcatg aaacaggttt
    10021 gcgtcctcgg taacgggcag ttaggccgta tgctgcgtca ggcaggtgaa ccgttaggca
    10081 ttgctgtctg gccggtcggg ctggacgctg aaccggcggc ggtgcctttt caacaaaacg
    10141 tgattaccgc tgagatcgaa cgctggccgg aaaccgcatt aacccgcgag ctggcgcgtc
    10201 atccggcctt tgtgaaccgc gatgtgttcc cgattattgc cgaccgtctg actcagaagc
    10261 agcttttcga taagctccac ctgccgaccg caccgtggca gttacttgcc gatcgcagcg
    10321 agtggcctgc ggtgtttgag cgtttaggtg aactggcgat tgttaagcgt cgcactggtg
    10381 gctatgacgg tcgcggtcaa tggcgtttac gtgccaatga aaccgaacag ttaccggcag
    10441 agtgttacgg cgaatgtatt gtcgagcagg gcattaactt ctctggtgaa gtgtcgctgg
    10501 ttggcgcacg cggatttgat ggcagcaccg tgttttatcc gctgacgcat aacctgcatc
    10561 aggacggtat tttgcgcacc agcgtcgctt ttccgcaggc caatgcgcag cagcaagcgc
    10621 aagccgaaga gatgctgtcg gcgattatgc aggagctggg ctatgtgggc gtgatggcga
    10681 tggagtgttt tgtcaccccg caaggtctgc tgatcaacga actggcaccg cgtgtgcata
    10741 acagcggtca ctggacacaa aacggtgcca gcatcagcca gtttgagctg catctgcggg
    10801 cgattaccga tctgccgtta ccgcaaccgg tagtgaatag tccgtcggtg atgatcaacc
    10861 tgattggtag cgatgtgaat tatgactggc tgaaactgcc gctggtgcat ctgcactggt
    10921 acgacaaaga agtccgtccg gggcgtaaag tggggcatct gaatttgacc gacagcgaca
    10981 catcgcgtct gactgcgacg ctggaagcct taatcccgct gctgccgccg gaatatgcca
    11041 gcggcgtgat ttgggcgcag agtaagttcg gttaactggt gctctattct tgccggatgc
    11101 ggcgtaaacg ccttatccgg cctaccgatc cggtacccat tgtaggcctg ataagatgcg
    11161 tcaagcatcg catcaggcat tgtgcaccaa ttgccggatg cggcaccggt tgtaggcctg
    11221 ataagacgcg tcaagcgtcg catcaggcat ggtgcaccga ttgccggatg ctctcatcac
    11281 tacagcgaaa tacaggtccc cgcttcgccc gccagcgtct cttcaattcg cgataacgcc
    11341 ccaatccacg cgggtttacc acggcttctg acataaccac ttaccgccgt taccttcggc
    11401 cccatcgaac catcggcttt ggcaaatggc gctaactcat ccggtgtggc atggcgaatg
    11461 gcgcgttgct gcggcgttcc ccagttttca tataccgcat cagcatcggt gaggatcacc
    11521 agcccatcgg cgttaatctg ctcggcaagc aaggcagcgg cgagatcttt atcaatcacc
    11581 gcttcactcc ctgccccatc ttccgtcaca ggcacaccgc cgccgccact gcaaatcacc
    11641 acatgcccct ctttgagcaa caactcgatg gcttcgctgt cgagaatttt acgcggttgc
    11701 ggagacgcca ccacccggcg caaatattta ccatcacgtt tcatctgcca gccgtaagcc
    11761 gcttccagtg cctcttgttc ttctggctga taaaccggac caataaattt ctctggctgc
    11821 aaaaacgccg gatcatcagg cgaaacctca atgcgcgtca gcaccgtcgt cacgggcggc
    11881 atctgcggct gtgcgctcaa actctgcgcc agcatatagc caatcatccc ctgactttct
    11941 gccaccagca catccagcgg atacggttct acctctttcc acgccagatt ctgcaatgcc
    12001 agcagcccca cctgcggccc gttgccgtga acaatcgcca gccgataaga acgggccagg
    12061 cgtgccagcg cgggtacagc actggcgata ttgcgatatt gattttccgc cgtcagcgcc
    12121 tcaccgcgct ggagtaaggc gttgccccca agagctacaa ccagtgtttt catggttttc
    12181 cttgtaataa ttgttgacca agccagaacc ccagcagtgt atctgcccct gacgtatgcc
    12241 ccagcgccag cagcgaatca atcgcaacag cggtacgttt cgggcaactc agagcatgaa
    12301 caaagtgcag gagtggcgag gcgaaatatc cttgcgcggc ataacgtaaa taactgacgc
    12361 tcaccgctgt ggtaacgagt tgcagattgt cggaacaggc aaaaaactgg cgaccggagc
    12421 gcgcatctaa agcgccataa taccaggccg ccagcagcat tccgctcagc gtgtcatcat
    12481 gactcggcgt taatccgggg cctttaccca gccagtgccg ccagtcggtc ttaacgccat
    12541 tgagcgcggc ctgaaaacag tgacgaaact ggcgtaactc agcaggcagc ggatcgcttg
    12601 ccgccaacgc cagtggcccg aaaagcccgg tttcctccgc gcgttgcatc catgcagctg
    12661 caagcggttg aggatgcgca ggcggcgtaa tacgcagcaa acaataacgc tgtggctgtt
    12721 taaccgtgaa acgcccgagg cgaatccctt gagccacaac ctgtggtcgt tcattgccgc
    12781 ataatccacc gcataacgca tcgaattgcg cgcggcgaag cacccatcct ccggggccga
    12841 aaccactacc ctgacgatgc agcgttaaca gttcgccgct ttccgtcatc aggttaatcg
    12901 cccgtcgcca cactcccgct aaccgccagg actggcgata attcggtgca ctacggctgg
    12961 caagcagagg atggatgatc gtcattgcgc gttcctcaac caatccccat gctttcagcc
    13021 agtgcttcca gcgcctgttc aaaacacgcc agcggtgccc gcacagtgcc tgcgccaatc
    13081 tgcccgatcc ccgcctcttt atgggcgata ccggtattga tgagtggcgt aatgccggtt
    13141 tctaccacgt gacgaatgtc cagtcccagg cacgcgccct gaaaatccca gcctgggatc
    13201 tgcaactgca tattgcgctc aaggtaaatt tccgccatct cttcagatac cgctctggcc
    13261 gcttccatgc cacccgcacc gacaaagcgc gttacgccag gcgctgcgat cattgccgca
    13321 cctccgatac caaaggtttc ggtaatcgcg ctatcgccca tatccgggtt cgcctgctcc
    13381 tgcgagaagc cggtgaaaaa cagaccttgc ggagtgttta caggcgcagt aaaccagcgt
    13441 tcgcccagcc cgcttacccg aatcccgaac atattgccgt tgcgggtcat tgccgtgacg
    13501 atgctgcctg cgcggatcat cgcgccagca tccatcgccg ccttgcagta agccatcgcg
    13561 aggttgagga agaactgatc ggtcacgctg aggaaatcca tcacttcggc gatgtgctgt
    13621 ttatcatgat cgaggcgagc aatttgtggg gccagcgcac gcattaacag tgctgaggaa
    13681 gcaatattgc gttgatggaa ctcatcgccc atggtaatgc cctgcgccat catcgccgtg
    13741 agatcgatac cgtgctccat gcgccccagc gccgcgctta atactggcat taacacatcg
    13801 cgcatccagc gatggcgagt caggacatct tcgccgtaag cgccaaaacg catcactttg
    13861 ccgatacctt cgttgaggtt gcagtacgcc cggttgccgt cggtcacgtt ctcgaccacc
    13921 agcatcggca tactggcaga agtaatgccg cccattggcc cgacggcatt cacatggtga
    13981 caaggaatga agttcacttc cccctgctcc agcattgcca gtgcctgcgc ttcatctttc
    14041 gcccagcctt caaacagaca tgcacccaca catgcccctt tcatgggtcc ggtcatctcc
    14101 tgccagcgca ttggcggccc ggcgtgaagc agtgttttgc cctggtttag ttcgctgata
    14161 agtgaagaag ccggttgcac atccagccag tgtggacgag cgcgacgaat ttgttcgata
    14221 accgcagcat tggcttgcgc cactgatgta aacatgggaa ccccttattg caaacgttct
    14281 aataaacgag ccagtttttt attgccgccg gcgactggcg accattggta atgcacaacc
    14341 ggtttgctgg cgctttgtag ctccagcgca aagctgcgta atccgatgtt aatcacggcg
    14401 acgttttcca gtaatgacgg tgtgtgttgc tgtgtggcag acgagagcgg atgaattaac
    14461 gctgccgcca gcaaggtggc ttccggtagc gaactcacta ccgcgatccc cgcatcttcc
    14521 agcgtggcga tttgctgcga gcggcattgc gggtcacgtt cggtacctgt caccgtggca
    14581 atggcgtaca gcggttggct gtctgaacgc gcggcacagg ctttttgcca ggcgctcacc
    14641 agcgaggcag caggatcggc ggtcgcaccg aagccaatca cgacatcaag cagcaacacg
    14701 cgcacttgcg gtttagcgcc gagatcggca attaactggt tgcgtaaggc tgggtcgatc
    14761 atcggatggg gacgcccgac ggtgtagaaa tcatcgccca gatcaatgat ctggtgacca
    14821 tcggcgtcca gcatcatgcc gtgatgatgg gtatcgtcgg cttccacgcc aaggtgcccg
    14881 gcaagtaatc ccgccgcttc ggcagccagc gtaccgccgg tatacaaacc gcaaataaat
    14941 ccgctgctgg caggcgttat cgcgttacgt cgcgccgtaa cgcgtgaaag caggcaagcc
    15001 aggcgtgcgg cctcatccag cgaggaggca aaccagacat tctcgtcgcg ggccaccgcc
    15061 ggggtataac ctaaaaacag cgccaccgtc ggtttgccgg ttgctttcat ggcattaaca
    15121 attttcagac gcacagcttc ggcaggcggt tttgaaacaa atgccagcac ttcgcttttc
    15181 tcgtctgcac tgagcatttc cagcgccgtc agcgcactga tgccgcccac ttcacggctg
    15241 aggtcgcgcc cgccaaggcc aatcgcgtga gtaattccct cccctgccag cgcaatctgt
    15301 gagcacagct cctgaatccc ggtaccggaa gcgccaatga cgccaatgtt gccttccggc
    15361 atcacgttag caaaagccag cggcgtggcg gcaatcatcg acgtaccgca gtccggcccc
    15421 atcaccagca agcctttttc tcgcgcgcgg gttttaagtt ggatttcatc ttccagcgtg
    15481 acgttatcgg agaacatcat cacgttgagg ttgcgatcca gcgcctgatt cgccagctcc
    15541 gccgcatact cgccagccac tgaaatcagc gccagattgg catcggataa tttctgacag
    15601 gcactgtccc agcgacgcac ctgcgtcaag gactggctgc tgcctgaccc ctgcgccagt
    15661 tgttttagcg cctcttcaag ctgctgcata atcgcctgcg cgatccccgc atccgccgct
    15721 tcgctacgaa ttgccacgca aatatcgttc ggcgtggcgt tattaaaatc gtcatgccag
    15781 aaacctgtgg tatctaataa cgctttattg gcgggcgtac ccatcattac ggaaacatca
    15841 tcaacatttt ctgattcgct gagttttcgt gaaataatca ttaaactgac cgaatcctga
    15901 aaacaccctt ttttaataaa ggcgtggatc ataacaactc cttaaaaatt gcacccatgg
    15961 caattgcacg agcagtgtta atgtggcgat tacggtatcg atgacaacga gtaaataagg
    16021 tgatgaatat cacacaggga ttattaacaa aagaattcaa ataaccgcca tgctattacg
    16081 tgttattttt taataagcgt ggttgtttta aaaaccaggt taaaagcgag cggattatca
    16141 aataaattct aactatattt ttttgcctgt ctggatcaca taatccagat attttccctg
    16201 tatgttaatt gcagtcatgc ttcacaccgt cgttaaaaag gaagacagat gaaaatcagt
    16261 cgggaaacac tccaccagct aattgagaat aaactctgcc aggctgggtt aaaacgtgag
    16321 cacgctgcaa ccgtggctga agtattggtt tacgccgatg ccagagggat ccactctcat
    16381 ggcgcggtgc gcgtggaata ctacgcggaa cgcatttcaa aaggcggcac caaccgcgaa
    16441 ccggagtttc gtcttgagga aaccgggccg tgctcggcaa ttttacatgc cgacaatgcc
    16501 gccggacagg tcgcggcgaa aatgggtatg gaacatgcca tcaaaaccgc ccagcaaaat
    16561 ggcgttgcgg tggtcggtat cagccggatg ggtcacagcg gcgcaatctc ttattttgtg
    16621 cagcaggcag cccgcgccgg attaattggt atttcgatgt gccagtccga tccaatggtg
    16681 gtgccgtttg gcggcgcgga aatttactac ggtactaacc cactggcctt tgccgcgccg
    16741 ggagaaggcg acgagatcct tacctttgat atggcgacta ccgtacaggc atggggaaaa
    16801 gtgctcgacg cccgctcgcg taatatgtct atcccggata cctgggcggt cgataaaaac
    16861 ggtgcaccaa caaccgatcc gttcgcggta catgctctgc tccccgccgc cgggccaaaa
    16921 gggtatggcc tgatgatgat gattgacgtc ctctcaggcg tcttactcgg cttaccgttc
    16981 gggcgacagg ttagttcgat gtatgacgat ttacatgccg ggcgtaattt ggggcaatta
    17041 catatagtta ttaatccgaa ctttttctcc tccagcgaat tattccgtca acatcttagc
    17101 cagaccatgc gcgaattaaa tgccattacc cccgcgcccg gttttaatca ggtttattat
    17161 cccggacagg atcaggatat taaacaacgc aaagccgccg tcgaaggcat cgaaattgtt
    17221 gatgatattt accagtattt aatttccgac gcgctttata acacgtcata cgaaacgaaa
    17281 aatccctttg cgcaataatt attgagacag gactttctta tgattacaca tttccgtcaa
    17341 gccatagaag aaacgctacc ctggctctcc tcttttggcg ctgacccagc gggtgggatg
    17401 acccgtttac tttattcgcc ggaatggctg gaaacccagc agcaatttaa aaaaagaatg
    17461 gcagcaagcg ggctggaaac acgtttcgat gaagtgggga atttatacgg tcgcctgagt
    17521 ggcaccgaat atccacagga agtggttctg agcggttcgc atatcgatac cgtggttaac
    17581 ggcggtaacc tcgacgggca attcggcgcg ctggcggcgt ggctggcaat tgactggttg
    17641 aaaacgcaat acagcgcgcc gttacgtacg gtcgaagtgg tggcgatggc agaagaagaa
    17701 ggcagccgct tcccgtatgt cttctggggc agtaaaaata tctttgggct ggcgaaccct
    17761 gacgacgtgc ggaatatctg tgatgccaaa ggaaatagtt ttgtcgatgc gatgaaggct
    17821 tgcggattta ctctgccgaa cgccccacta actccgcgtc aggatattaa agcctttgtt
    17881 gaactgcata ttgaacaggg ctgtgtgctg gaaagtaatg gtcaatcaat tggcgtggtg
    17941 aatgcaattg tcggacagcg tcgctatacg gtgacgctga acggcgaatc aaaccatgca
    18001 ggcaccacgc cgatgggtta tcgtcgtgat acggtttacg ctttcagtcg catttgccat
    18061 cagtcgatcg aaaaagcgaa aaagatgggc gatccgctgg ttctgacctt tggcaaagta
    18121 gagccgcgtc cgaatacggt gaatgtggta ccgggtaaaa ccacgttcac cattgattgt
    18181 cgtcataccg acgctgccgt gctgcgcgat ttcacccaac agttagaaaa cgacatgcgg
    18241 gcgatttgcg atgaaatgga cattggtatt gatatcgatt tatggatgga cgaagaaccc
    18301 gtgccgatga ataaggagct ggtcgccacc ctgacagaat tgtgtgaaag tgaaaaactg
    18361 aattaccggg tgatgcacag tggtgccggg cacgacgcgc aaattttcgc gcctcgcgta
    18421 ccaacctgca tgattttcat tcccagcatc aatgggatca gccataaccc ggcggaacgc
    18481 accaatatta ccgaccttgc cgaaggggtc aaaacgttgg cactcatgct ttatcaactt
    18541 gcctggcaga aataaggagt cataaatggg atatttaaat aacgtcaccg gttaccgcga
    18601 tgatttactg gctaaccgtg cgattgttaa acacggtaat ttcgcgctgt taaccccgga
    18661 tggtctggta aaaaatatta ttccgggctt tgaaaattgt gacgcgacaa tcctctccac
    18721 gccaaagctg ggtgcctctt ttgttgatta tctggtcaca ctgcatcaaa acggtagcaa
    18781 ccaacagggc ttcggtggcg aaggcattga aacgttcctg tatgtgatct ctggaaatat
    18841 cactgccaaa gccgaaggca aaacatttgc cttaagcgaa ggtggctatc tttattgccc
    18901 accaggctcc ttaatgacgt ttgttaacgc ccaggccgaa gacagccaaa tctttttata
    18961 taagcgccgc tatgttccgg tagaaggcca tgcaccgtgg ctggtttctg gcaatgccag
    19021 cgaactggaa cgcattcact atgaaggtat ggatgatgtg atcctgctgg attttctgcc
    19081 caaagagtta ggttttgata tgaacatgca tatcctctct tttgcaccag gtgccagcca
    19141 cggttatatc gaaacacatg ttcaggaaca cggtgcctat attctttccg gtcagggggt
    19201 ttataacctc gacaataact ggatcccggt gaaaaaaggc gattacatct ttatgggcgc
    19261 ttattcttta caggctggtt atggcgtagg gcgtggtgaa gcgttcagct atatttactc
    19321 gaaagattgt aaccgcgacg tcgagattta agtcatacca ctcgcctgat tatttttagc
    19381 tatgttggat tttgccacgg tttataccgt ggcttttttt gtagtaaatt caggtaaaaa
    19441 aagatgcgat ataaatcgca tccgctttaa aggtaagggt cagtttttaa ttccctgacc
    19501 tattttaatg gcgcaggcaa tatttcgtgc gctgttgaag agattggttt caccgctggc
    19561 gagcacttcg gctaaaggtg ccagacgtgg caaaatgctg aataccgcat caatgccgta
    19621 ctggtgcacc acttccacgc catcacccaa tacgccagca atcccaatca ccggtacatt
    19681 aaactgcttc gccaccgacg ccacacccag cggcgcttta ccgcctgccg tttgcgagtc
    19741 gatgcgccct tccccggtaa tcaccagtgc tgcgccctgc actgcctgcg caagattgac
    19801 cgcattcaac acaatctcaa tacctggttt aatatccgca ttgagaaata ccgccgccgc
    19861 aatacccata ccgcccgcag cgccgccgcc cgccatctgg cagacgttga tttcagtttg
    19921 ctgttgtaaa acacgggcgt aattttgcag cccctgttcc agctcttcga ccatttccgg
    19981 cgttgcccct ttttgcgggc caaacaccgc cgccgcgccg cgtgccccta ccagcgggtt
    20041 atcaacgtca catgctactt caatatggca attcgccaga cgcggatcgc attcatccat
    20101 ctcaatgctt gccacgcgcg ctaaattacc gccgttagcc accagcacct gaccgtcggc
    20161 atcaaggaaa cgcacaccga gcgcctgcgc catgcccata ccaccgtcga ccgtcgcact
    20221 gccgccaatg ccgagaataa tatggcgaat gccgttatcc agcgcatgac gaattaactc
    20281 ccccgtacca aaactggagg ccagcaacgg attacgcttt tcaggcgcga ccagcatcag
    20341 gccacttgct gccgccatct caatcaccgc cgttttcccg tcgccggtaa ggccataaaa
    20401 agcattcact ttttcgccca tcggcccgca gacttcaagc gtcacgatgt tgccgcccgt
    20461 cgcggcgacc atcgcatcca ccgtcccttc gccgccatcc gctatcggca aacagatata
    20521 gttcgcatcg ggaaagaggg tcgaaaaccc ggctttaatt gcctgacaac atttttctgc
    20581 acttaagctc tctttaaaag agtctggcgc aatgacaatc ttcattatca atcctttttc
    20641 aacaaattat tcgatgcgtt cccacggaat aagattatcc ataagaacag ccagtaaaat
    20701 cccgaccagt aagccgttac tgagcagagg acgcagcgtt aatggaaggt cttgcagata
    20761 cacaggcggt aatgccatta aaaatatgcc gacaaataac ggcaatgcga gtcgataaat
    20821 attgcgagcg gtaaacgtta tttgctggct aaacaccagc gcggaaaaga gtaaaggcag
    20881 ataagaaacc agcatgaccg cactactcac gggtaaaggg atactgcaaa acagtcgtgt
    20941 gagtgccgga accagcgcca ccagaaggca aataacgctg ccataaataa acgaacgccg
    21001 cgtgtaatcg cctgtctggg ttaataaacc aatggatgaa acaaacggtg aaaatggaat
    21061 taccgccagc ggtacggtta tcagcgtcat aaatccggtc gccacaaagc tgcgacgata
    21121 acgcgtattc cccgcgccct gctgcggata aaaaacatcc gtgccccgaa tcgcaccgta
    21181 ggtattgctg atattcacca gacctgtaat caccgccgtc agaataattc ccggagacaa
    21241 agcacctcca ctgccgagcg ggaaccactg ccagtgcaac tcaccggaga gcgagtgcga
    21301 agaaggaaag caaaagtacc acaacaacca gccggttatg gtgccgacca gcaggccata
    21361 acgggcaaaa cgttgcggca ggaagataat catccccagt accaggcaca tcaccgccac
    21421 cgagagcgcg aacggcggta actgaatttt aaaatttggg tcggctatgc caaacggcag
    21481 cccgagcata cctttgaaaa agatagtggt cagctgcgcg cccagcatca acataaacaa
    21541 gaccatcacc gacggcgtaa ataaccgtgc caggcgatgg cctaatccgc taaaaccaat
    21601 caacatcgtc agcacgccgg agagcgcaat ccccaccgcc aggctggtag cgatatcgtt
    21661 gatcggtgtc ccgcgcgatg cttcgccaag ggtgatagta aggatggttc cccaccactg
    21721 gccacccggc ccttccataa tagcgcgacg atgaccgcag aacgcctgag cgaagcaggc
    21781 cagtgcggta gcaagaaaag catattgcgt gagcgtaagc aggctacttt gcggcaactg
    21841 aaaagcagaa agtaacgtag gaggaaccac aaccgtgttg caaaaaataa agaaaaacca
    21901 ctgaaatcct gataacaggc tttcgcggct gactgcaaaa ttgaacataa acacacctta
    21961 accggagaat gcccgccgca aggacgggca ttgcaggggc cagattactg ctgatgttta
    22021 aggataaatt gacctttcgg cgcaacaggg aagccctgtt caatgtcgta aatcacatca
    22081 ccacgtaaga tggttttcgt gatacgcgcg ccaatggtac ggccaacata cgggctgact
    22141 ttgtggcgat attccagatc gtcattggta agaacatagc tgctattcgg ctgaatgaag
    22201 acgaagtcgg catcttttcc tggggcgata cggccttttt gctgcagacc gaaaatatct
    22261 gctgcgttag tcgccattaa tttgccgaac attggcagag acattccgcg tttctgtacc
    22321 gcttcatcga acatcacgtc catacagttt tgcagaccgg caataccgcc ccatgcttcc
    22381 atgatgttgc cggctttcat ttccggaggg catggtgagt ggtcggaaac caggcagtcg
    22441 atttcaccgt taaacagttt ttcccacatg cctttctggt tttccagatc gcggatcggc
    22501 ggtgaacact tcgccagagt gccgatttct tcgaactgat cggtatccag tacaaagtaa
    22561 tgcgggcagg attcacaagt aacgtcctga ccttcctgac gtgcacgagt cacttcttca
    22621 acgccttccg ggctgctgat atggcaaacg tgcagacggc aaccggcaac tttcgccaga
    22681 tacagcacgc ggcgaatcgc ttccacttcg gtaaataccg gacgcgaagc cacatagtca
    22741 tgggcagtta cgcgaccttc acgtttcgct tcttcgccca gtgcgtcaca gatcagcgcg
    22801 ttttcgcagt gcaccagcac cggctgcccc agttcgccca gcttctgcgc acctttgaag
    22861 aactgccagt cgtttacatc acggaagtcg ttgtcgatac cgcgatcgcc acaggtcgca
    22921 acgaagcatt tgaagccgac aacgcccact tcatccagct catgcagacg gtcgatgttg
    22981 taagacacca ggccaccgag ttgtgccgca tcgatagtca gcttgccttt agcggcatcg
    23041 aacttcagtt caattgaagc gcggtcaacc gttgcaggca gctggttgag cggcatttcg
    23101 atcatggtgg tgataccacc ttttgccgct gcgcgagtac cggtttcata accttcccag
    23161 tggctacgac ccggttcaga aatatgggtg tgcgcatcaa ccatgcccgg cgaaaccacc
    23221 agaccagacg catccataac ttcttttgca tcgcccagat cctgaccgat agcagcaatt
    23281 ttcccgcctt taacggcgat atctacaacg cgagcttcgt tttctaaaat aacggtgccg
    23341 tttttaatga ttaaatcaaa agacataaca aactccttgt aaaatacaga ttggttttta
    23401 actcaatgtt caatatcggg attaattaac ctatggtttt ttgctctcct gttttttctg
    23461 ctgttgtacg tttctttaat aaggcgtagg ccgcaaaggc gacgatgacg ccgacaaacc
    23521 atgaaacacg cgataacggt tccataaagg gaataaactt accgccaaga gataaaataa
    23581 cggccaccag agttactgaa aacgcggtga ggttaaaacc gttatcgtaa tatttataat
    23641 cgccaggtgc ggtgtacagt tcatcaagat taatttgccc acgcatcacc acaaaataat
    23701 gtgccatcat gacaccaatt accggaccca gcattccgcc gatgatatcg aggaacagat
    23761 aaatactgtc ctgattttcc attaatttcc acgggcagat cagcaggctg ataatactgg
    23821 caatcagtac gccgttttta taggtcagtt ttgtcggtgc aatggcagca atctgataac
    23881 cggccggaat aatattaccg gtggcgttag tggaaatagt cgtcatcaga ataaccagta
    23941 ccgcaaagaa cgaggcaaac aggctgtccc aacgctgaac aatatccagc acgttccagg
    24001 tgtcagcgcc gtagtgaata ctggctccgg caataataca taccccagcg accgcaaaca
    24061 gaatataggc cacaactaaa cccagcgttt gccccagcgc ttgctcacga aacgagtggg
    24121 cgttttgcgt aaagtcggat gcgctcaccg ccggtgccgc ccagaccgca actaccgcgt
    24181 taatcaccac caggaacaag aatccactgt tttctgcttt ctgaataccg ctcggaatgt
    24241 agtcaaagat tggaccgatc ccgaccagtg aaatcgccca aatcgccatg ccgccgaaaa
    24301 cgatatagat gcacgggtta agaatggcag tgaatttatt taaaactttg ccaccgccaa
    24361 aaccaatacc aacgttaacc agccagaaga gtaagaaagt aattaagccc ggtagcgaaa
    24421 ggcctaacaa ggtaaaatca ccaccgagag ttaaaaatcc cggccagatt ttgccaatca
    24481 ggatcaagca ggccagtgat ccggcgtaac attgcaggcc aaaccacata atggcagcaa
    24541 taccacctct taataatccg ggaaacagtg cgccacgtac gccgtaagaa gcacgcagga
    24601 tcatggcaaa cggtacgccg tatttactgc ccgcagcacc gtttaatacc attaccgcgg
    24661 caatgaaaaa ggcgctgagg ataattgcca gcataatact aaaggtagac aagccgagaa
    24721 taaaaaagcc gccgaccatc acataattgg gaacgttatg aaccgaaccc atccataagg
    24781 taaaatagtt aaatgttttc caggtccgct ggctttgcgt tttcggcaat aaatcttcgc
    24841 tatagccgcg ttgctggaat agctttctct gatgttccat aaatccctct taatttcacg
    24901 tatcgaataa cgttgtttta atctagctat gaggaacaaa taaaataaaa aatattattt
    24961 ttaatactga agcaacatct ttggctgctt tcttttttaa tattgctggc cgattcattt
    25021 tctgatatcg gccattttta ttgcgggtat caggccagtt tatggttagc cattaattcc
    25081 agcgcctgca ctaacgcaga gtgatccaac tggctgccac cgtttgccgc acaggtatta
    25141 aataactcct ggcaggtcgc agtgtttggc aggttcagcg caagtgcttt cgcactttgc
    25201 agtgccaggt tgagatcttt ctggtgcaga gcgattttga agcccggatt aaaggtgcgt
    25261 ttaatcatac gctcgccatg aacttccaga atacgtgagg aagcaaagcc gcccatcagc
    25321 gcctggcgca cacgtaccgg gtccgcaccg gcttttgaag caaatagcag ggcttcagaa
    25381 accgcttcaa tattgagcgc cacgataatc tgatttgcca ctttgcaggt ttgaccatcg
    25441 ccgttaccgc ccacgagggt gatattttta ccgagcagtt caaacagcgg tttaacacgt
    25501 tcaaataccg cttcatcacc gccaaccata atcgacaacg tcccttcacg cgcaccgatt
    25561 tcaccgccgg agactggcgc atcgagataa tcgccgccca gttcattcac ctgacgagcg
    25621 aaacgcttag tttcaatcgg ggaaatggag ctcatatcaa caatggtttt gcccttcagc
    25681 gaagtttttg cacagccatt ttcaccaaac aatacttctt caacctgcgg cgtatctggc
    25741 accataataa agataatgtc agaagattca gttacctggc gcgcggtttc aacatttact
    25801 gcgcccagcg ccagtaattc ttccgcaacc ggaccaatgg tcgtcacatg cagttgatgg
    25861 cctgcgcgtg ccaggttaac tgccatcggt gtgcccataa tacccaggcc aataaatccc
    25921 agtttcataa ttaacctctt gtgaatatcg ctgtttctga attcagacaa cacgatcccc
    25981 tgccggcata gcggcaaaaa atgcattgtc tgaatcgttt ttcgttttag cggtatggat
    26041 taatccatga taatcccgct tccgtggtgg tcaggggttt atattcgcac cctacccaac
    26101 cgtcataatc agacttttca attaaatcga ataaaaagtc gtaatttatt tccccggtgc
    26161 caggttcgcc acggcggggg ttatcggcaa tctgcaaatg accgatttta tttgcccatg
    26221 tagtcatggt ttgggctaat tccccttcca tgcgttgcat gtggtaaata tcgtactgga
    26281 tcttgatatt gttactgcca atatcggcaa tcagcgccag tgcttgttgt gttccggtca
    26341 aatggaagcc cggcatatca aagtgattaa gtggctcaat cagcagcaaa ataccctctt
    26401 ttgccagcat atttgccgca tagcgcaggt tctctaccag agtggtatgt acctgctctg
    26461 ctgatacgcc cacaggtgtt ttaccaacca gacagttaat ttttttatta cccagtgcac
    26521 gggcataacg aatcgcaacg gctacaccat cacgaaattc ctcttcccgg ccaggaatac
    26581 aggctattcc gcgttcaccc gccgcccagt cacccgcagg taaattatgc agcgtatgct
    26641 ccaggttatt actgctgagc ttgtgctgca aaacttctat gtcgtagtca taggggaaca
    26701 taaattctac agcgcgaaat ccgcactgcg ccgctttatc aaagcgctca agaaaatcat
    26761 attcctggaa taacatcgac aagttagcag agaaacgtaa cattaattac ctcccttact
    26821 tatattgcat aaaacaggtt tcggttggcg cgtccgctgc gttatcggcg atatcttcaa
    26881 attccatcac attatcgagt tcgttaccca tcgaaatatt ggttacgcgc tcgagaatga
    26941 tttccaccac cacaggtaca cggtattgat tcattaatgc tttcgcctgt tcaaatgccg
    27001 gagcaatatc ttccggtttg aaaacacgaa tcgctttaca acctaaacct tccgctactt
    27061 ttacgtggtc aacaccgtag ccattcactt cactggagtt gatattctcg aaagcgagtt
    27121 gcacgcagta gtccatatca aacgcgcgct gcgactggcg aatcagcccc agataagcgt
    27181 tattgaccag tacatggatg tacggaatgt tgaactgtgc gccaacggcc aattcttcaa
    27241 tcaggaactg gaagtcgaag tcaccggaaa tcgccaccac tttgcgctct ggatcagcgg
    27301 cacaaacccc cagcgccgcc ggaatggtcc agcccagcgg gcctgcctgc ccacagttga
    27361 tccagtggcg gtctttaaag acatgcagca tttgcgcagc ggcaatttgc gacagaccaa
    27421 tggtggtgac atagcaaaca tcgcgaccaa aggctttgtt catctcttca tacacgcgct
    27481 gcggtttcac cggcacgttg tcgaaatggg ttttgcgcag caaagtgcgt ttgcgctgct
    27541 ggcagtcggc gacccattct ttacgacacg gcagacgacc cgctttttgc atctcctgcg
    27601 ccacttcaac cagcagtgtc agcgccgctt tagcatcaga gacaatgccg agatccggac
    27661 acagcacgcg accaatttgc gtcggctcaa tatcgatatg aacgattttg cgcccttcgg
    27721 tgtatttctc taccgaaccg gtatgacggt tagcaaaacg gttaccgata ccaaacacca
    27781 tgtcggacgc cagcaacgtt gcgttaccgt aacgatgcgc ggtttgcaga cccaccatcc
    27841 cggccatcag ttcatgatcg tccgggatac agccccagcc catcagcgtt gggatcaccg
    27901 gaacgctggt cagttcagca aactgttgta acagtgcagc tgcgtcagca ttaattaccc
    27961 cgcccccggc aacaatcacc ggacgttcgg cctggattaa catttctaca gctttttcga
    28021 tctgcatacg gctggcagca ggtttgtaga ccggcagcgg ttcgtacata tcaggatcaa
    28081 actcgatttc cgcaacctga acgtcgaacg gtaaatccac cagtaccgga cccggacgac
    28141 cagaacgcat caggtgaaat gcctgttgca acacacgagg caccagcgcc gcttcacgta
    28201 ctgtaaccgc cattttactt accggcttgg cgatagcttc gatatcaaca gcctggaaat
    28261 cttctttatg cagacgggcg cgcggagcct ggccagtaat acacagaata ggaatggaat
    28321 cagcagaagc agaatagagc gcggtgatca tgtccgtgcc cgcaggaccg gaagtcccca
    28381 gacatacgcc gatattccct gccgttgcgc gggtataacc ttccgccatg tgcgaagcac
    28441 cttccacatg acgcgccaga atgtgacgaa taccgccgtg cttacgcatc gctgagtaga
    28501 acggattgat tgcagctccc ggaacgccga aggcggtggt gataccttct ttctccagca
    28561 catacattgc cgcgtcaacg gctctcattt ttgccatttt attcctacct ctattaattg
    28621 gaaaaatttt ccaactttca aattttgtca aaaaaacccg ctccgcagag cgggatgcga
    28681 cagacactat ggatgtgctt tcagtcccaa cgccgtgctg atatcgcggg cggtgtctct
    28741 gaccagctca ccctgactga caaaacgatc ttctgtcagt cttgatgaag gcccggagat
    28801 ggagatagcg gcaacaacgc taccgacatc atcgtaaatt gctgaagcta tgcaattcag
    28861 acctacaaca tgctcttctt tatctacggt atagcccagt tcacgcgctt gttccaggtc
    28921 cttcagcaag gtgggcatat ccacaagcgt agttggcgta aactgctgca aaccggtttg
    28981 cagaatgatg ctcatcaact cctcttccgc cagcggataa agcagcgctt tgcccgcacc
    29041 ggaagcatgc agcggcagac gactgcccag tggcgcacac atcctgacca ttgatttaca
    29101 ctctaactga ccaattaata ctgcttcatt gccgttacgg atcgcgacat tgaccgtttc
    29161 gccggaaagt aacatcaggc ggcgcataaa cggcccggcg acggagagga catcgcggtt
    29221 atggatgtac gccgcaccga cgttaaagac acctaatcct atatgccacc agcctaattg
    29281 actgtcctga tagacaaaat ccgctgcctg taaaaccttc agcaagcgaa aggtcgtgga
    29341 gagcggcaaa tccagattga gagaaatatc gctaaccgac gaacttcccc cacttttttc
    29401 cagatattgc agaatcgcaa ttccccgctc taacgcctgt gcgcccttct gtgccacagg
    29461 ctccgcctgt cctggcctgc cgcgccgtct aacttccgtc atacctacgt taactccgac
    29521 ctacaagtga gcataaacaa tgaacaatta gtttatctta tgcaagtcgg ttgcaggcgt
    29581 cacgcaaaac agagttcctg ttcaggaata ctttcaacat cacagttgtc actgccgccg
    29641 cgatcgatgg tcagaaaatc ggtgacgcgc tgccaggcga aaagtgggtg atgccagacg
    29701 ttacgatggt aattcactcc ctgttcgccg ttggtgataa acgcccgcag cgttgacagg
    29761 tctggtttgt cgtcgcctaa cgccacgacc accacaaaca cttcaccttt catcgggata
    29821 aaggcctgag tgcccagcgg atgacgttcg agttcgtaaa tggtcagcgg cagattcgcc
    29881 ggttgcgcgc ggttaatgct gataagcgta cggtcttgct caagaatctc aaccagcgcc
    29941 aaatcgtggt aacgctccac caggccattg ttaatatgga aaaaatcccg ttgctgagtt
    30001 tcgattacgt cgccataagc actaaaggct tcctgactta acggtaatac ctgaagtttc
    30061 atctttttgt ccttgtaaca taattccgtt caaccatcgc cgaccggtca gcttggtttc
    30121 aataacaggc aatctaaaac cttaagagaa caacatcaat acaaaagttg gaatattttt
    30181 ccaaaactag atttagtcca caaattgaac agggttatca tgtaaaaata ctgataaatc
    30241 agaggataag aaaatcacgc aacttgcgga tttaaaaggg gatacctgaa gatgttcgat
    30301 ccagaaacct tgcggacttt cattgcggtt gctgaaacag gaagtttttc aaaagcggca
    30361 gaacgattat gtaaaaccac ggcgacgatc agttatcgca ttaaacttct ggaagagaat
    30421 accggagtag cgctgttttt ccgcacgact cgcagcgtga cgttgacagc ggctggcgag
    30481 catctacttt cccaggccag agactggctg agctggctgg aaagtatgcc aagcgagctg
    30541 caacaggtga atgatggcgt ggaacgccag gtgaatattg tcatcaacaa cctgctctac
    30601 cacccccagg ccgtcgccca gttgctggcg tggctgaatg aacgttaccc ctttacccag
    30661 tttcacatct cccgacaaat ctatatgggc gtctgggatt cgctattata cgaaggtttt
    30721 tcactggcta tcggcgtcac gggaaccgag gcgctggcaa atacctttag tcttgatccc
    30781 ttaggatcgg tgcaatggcg ctttgtcatg gcggcggatc atccgctggc gaacgttgaa
    30841 gagccgctaa cagaagcgca gttgcggcgc tttccggcgg tcaatattga agacagcgcc
    30901 cgcaccttaa ccaaacgcgt cgcctggcga ttgcctgggc aaaaagagat tattgttcct
    30961 gatatggaaa cgaaaatcgc cgcccatctt gcgggcgttg gcattggttt tttgccaaaa
    31021 tcgctttgcc agtcaatgat cgataatcaa caactagtta gccgggtaat cccaacgatg
    31081 cgccctcctt cgccattgag tctggcatgg cgcaaatttg gcagcggcaa agcggtagaa
    31141 gatattgtga ccttgtttac ccagcgcagg ccggaaatca gcggattttt agaaattttc
    31201 ggcaacccac gcagttaaaa atcaagtttc cttgcgcgtt ggcgcgtaaa atagcgcgca
    31261 agctgatttg aggttcactt ttaactatgc aagagagaca cacggaacag gactatcgtg
    31321 ccctgctgat tgctgatacg cccattattg atgttcgcgc ccctatcgag tttgagcaag
    31381 gcgcaatgcc cgccgctatc aatctgccgt taatgaataa cgatgaacgc gccgccgttg
    31441 gcacctgcta taaacagcaa ggctcagacg cagcgctggc gctggggcat aaactggtgg
    31501 cgggtgaaat tcgtcagcag cgcatggacg cctggcgggc agcgtgcctg caaaatccgc
    31561 aaggtattct ctgctgcgcc cgtggcggtc agcgctcaca tattgtgcaa agctggttgt
    31621 atgcagcggg gattgattat ccgctggtgg aaggcggtta taaggcactg cgccagaccg
    31681 cgattcaggc gactattgaa ctggcacaaa aaccgatagt gctgattggc ggttgtactg
    31741 gcagcggtaa aacgctgtta gtgcagcaac agccgaacgg tgttgatctg gaagggctgg
    31801 cgcgccatcg cggttcggcg tttggtcgca cgttacaacc acaacttagc caggcgagtt
    31861 ttgaaaacct gctggctgcc gaaatgctga aaaccgacgc ccgtcaggat ttgcgcctgt
    31921 gggtgctgga agacgaaagc cggatgatcg gttcgaatca cctgccggaa tgcctgcgcg
    31981 agcgaatgac tcaggcggcg attgcggtgg tagaagatcc gtttgagatc cgtcttgagc
    32041 gcctgaacga agagtatttc ttgcgtatgc atcatgattt tacccacgct tatggcgacg
    32101 aacagggctg gcaggaatat tgcgaatacc tgcatcacgg actttcggcg attaagcgtc
    32161 ggctggggtt acagcgctat aacgaactcg ctgcccagct ggatacggcg ctgacaacgc
    32221 aactcaccac cggcagcacc gacggtcatc tggcctggct ggtgccgtta cttaaagaat
    32281 attacgaccc aatgtatcgc tatcagcttg agaaaaaagc ggaaaaagtt gtctttcgcg
    32341 gtgagtgggc ggaagtggcg gaatgggtta aggcgcggta atgagggacg tatttgtatt
    32401 agtcgcgact tgatctgaca catggccttg aaaggttgag agttaccggt tttgatatga
    32461 gtgtcgaatc cttatccaaa acaagaggta actctcatgc ttcatactgc caatcccgtc
    32521 atcaaacaca aagccggttt gctcaatctg gctgaagaac tcagcaacgt atcaaaagcc
    32581 tgtaaaatca tgggcgtttc gcgtgatacg ttttatcgtt accgcgaact ggtcgctgaa
    32641 ggcggtgtgg atgcgcagat taatcgtagt cgccgcgctc ctaaccttaa gaaccgtacc
    32701 gatgaggcaa ctgaacaggc tgttgttgat tacgccgtcg ctttcccgac acatggtcag
    32761 caccgggcca gcaacgagct gcgtaaacag ggggttttat ctccgacagt ggtgtccgtt
    32821 ctgtctggtt actccata
//
LOCUS       DAPHDW010000039        32288 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.039, whole genome shotgun sequence.
ACCESSION   DAPHDW010000039 DAPHDW010000000
VERSION     DAPHDW010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32288)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32288)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32288
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.039"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(363..457)
                     /locus_tag="RH057_001825"
     tRNA            complement(363..457)
                     /locus_tag="RH057_001825"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(421..423),aa:Sec,seq:tca)
     gene            750..2132
                     /locus_tag="RH057_001826"
     CDS             750..2132
                     /locus_tag="RH057_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
                     /protein_id="HDV3128204.1"
                     /translation="MKSEVLSVKEKIGYGMGDAASHIIFDNVMLYMMFFYTDIFGIPA
                     GFVGTMFLVARALDAISDPCMGLLADRTRSRWGKFRPWVLFGALPFGIVCVLAYSTPD
                     LSMNGKMIYAAITYTLLTLLYTVVNIPYCALGGVITNDPTQRISLQSWRFVLATAGGM
                     LSTVLMMPLVNLIGGDNKPLGFQGGIAVLSVVAFMMLAFCFFTTKERVEAPPTTTSMR
                     EDLRDIWQNDQWRIVGLLTIFNILAVCVRGGAMMYYVTWILGTPEVFVAFLTTYCVGN
                     LIGSALAKPLTDWKCKVTIFWWTNALLAVISLAMFFVPMQASITMFVFIFVIGVLHQL
                     VTPIQWVMMSDTVDYGEWCNGKRLTGISFAGTLFVLKLGLAFGGALIGWMLAYGGYDA
                     AEKAQNSATISIIIALFTIVPAICYLLSAIIAKRYYSLTTHNLKTVMEQLAQGKRRCQ
                     QQFTSQEVQN"
     gene            2142..4460
                     /gene="yicI"
                     /locus_tag="RH057_001827"
     CDS             2142..4460
                     /gene="yicI"
                     /locus_tag="RH057_001827"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HDV3128205.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLSNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPAWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPTDVQWFDGSDPQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHSFLSLPIYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPATDGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQDGSQAESEQGLVVKPQGNALTITL"
     gene            complement(4513..6222)
                     /locus_tag="RH057_001828"
     CDS             complement(4513..6222)
                     /locus_tag="RH057_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HDV3128206.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPATLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPKL
                     PLTGDGNIQLTASGDIQANVPLKPTVSGQLHAVNAAKQQVTQTMNAGIVSSGEVTSTE
                     PVR"
     gene            complement(6343..7734)
                     /gene="xanP"
                     /locus_tag="RH057_001829"
     CDS             complement(6343..7734)
                     /gene="xanP"
                     /locus_tag="RH057_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HDV3128207.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            8014..9219
                     /gene="gltS"
                     /locus_tag="RH057_001830"
     CDS             8014..9219
                     /gene="gltS"
                     /locus_tag="RH057_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV3128208.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSMMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            9222..10088
                     /locus_tag="RH057_001831"
     CDS             9222..10088
                     /locus_tag="RH057_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128209.1"
                     /translation="MKKRRKEPETLREHCRHIFGDEPPVLCVWETEFDYADAELKALA
                     AKEWQQISERDLSAYYVLNLVYNEPMQIELFRYLFPLCLAQWHETVLAGGYGDHFEES
                     LMKALCRPYLWQEMMNASQRQQVRQFLLDTALQRMDNERGFNNVLCWLAVFNTLGGAA
                     PLIRSLWSRWWALDTPGKAVCAIQYAAHLIYPIEANPLWSQEWIGWGHPLGHKDGWSS
                     DNRAFLRQMLTPEMIVAGVQAAAEILRGEPEGAMAARIAQDAYEAMDILTIQIEDLLR
                     DLSCDESGHALE"
     gene            complement(10073..12154)
                     /gene="recG"
                     /locus_tag="RH057_001832"
     CDS             complement(10073..12154)
                     /gene="recG"
                     /locus_tag="RH057_001832"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HDV3128210.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARV
                     VAEIERDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFEPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPEMERYSNA"
     gene            complement(12160..12849)
                     /gene="trmH"
                     /locus_tag="RH057_001833"
     CDS             complement(12160..12849)
                     /gene="trmH"
                     /locus_tag="RH057_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HDV3128211.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGRGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            complement(12856..14964)
                     /gene="spoT"
                     /locus_tag="RH057_001834"
     CDS             complement(12856..14964)
                     /gene="spoT"
                     /locus_tag="RH057_001834"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HDV3128212.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            complement(14983..15258)
                     /gene="rpoZ"
                     /locus_tag="RH057_001835"
     CDS             complement(14983..15258)
                     /gene="rpoZ"
                     /locus_tag="RH057_001835"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HDV3128213.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQEQEAAELQAVTAIAEGRR"
     gene            complement(15313..15936)
                     /gene="gmk"
                     /locus_tag="RH057_001836"
     CDS             complement(15313..15936)
                     /gene="gmk"
                     /locus_tag="RH057_001836"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HDV3128214.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            16194..17876
                     /gene="ligB"
                     /locus_tag="RH057_001837"
     CDS             16194..17876
                     /gene="ligB"
                     /locus_tag="RH057_001837"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HDV3128215.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFGSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MVDKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVPQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQDDSDTLNSL
                     GVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWEEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNSLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARKQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            complement(17873..18490)
                     /locus_tag="RH057_001838"
     CDS             complement(17873..18490)
                     /locus_tag="RH057_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HDV3128216.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(18782..19606)
                     /gene="dinD"
                     /locus_tag="RH057_001839"
     CDS             complement(18782..19606)
                     /gene="dinD"
                     /locus_tag="RH057_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HDV3128217.1"
                     /translation="MNEHHQPFEEIRHYGTEGQEFWSARELAPLLDYRDWRNFQKVLA
                     RATQACEASNQAASDHFVETTKMVVLGSGAQRELEDVHLSRYACYLVVQNGDPAKPVI
                     AAGQTYFAIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDGVNSKQQANTTHFDVGSKVRQTIQELGGTMPEELPTPQVSIKQLENSVKITEKK"
     gene            complement(19827..20690)
                     /locus_tag="RH057_001840"
     CDS             complement(19827..20690)
                     /locus_tag="RH057_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HDV3128218.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMQSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            20817..21533
                     /gene="rph"
                     /locus_tag="RH057_001841"
     CDS             20817..21533
                     /gene="rph"
                     /locus_tag="RH057_001841"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HDV3128219.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAVCDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            21599..22240
                     /gene="pyrE"
                     /locus_tag="RH057_001842"
     CDS             21599..22240
                     /gene="pyrE"
                     /locus_tag="RH057_001842"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HDV3128220.1"
                     /translation="MKPYQRQFIEFALGKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGLEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RSEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(22277..22873)
                     /gene="slmA"
                     /locus_tag="RH057_001843"
     CDS             complement(22277..22873)
                     /gene="slmA"
                     /locus_tag="RH057_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HDV3128221.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(22980..23438)
                     /gene="dut"
                     /locus_tag="RH057_001844"
     CDS             complement(22980..23438)
                     /gene="dut"
                     /locus_tag="RH057_001844"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HDV3128222.1"
                     /translation="MMKKIDVKILDPRVGKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRDQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            complement(23416..24636)
                     /gene="coaBC"
                     /locus_tag="RH057_001845"
     CDS             complement(23416..24636)
                     /gene="coaBC"
                     /locus_tag="RH057_001845"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HDV3128223.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            24808..25476
                     /gene="radC"
                     /locus_tag="RH057_001846"
     CDS             24808..25476
                     /gene="radC"
                     /locus_tag="RH057_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418095.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HDV3128224.1"
                     /translation="MKNNSQLMMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            25693..25929
                     /gene="rpmB"
                     /locus_tag="RH057_001847"
     CDS             25693..25929
                     /gene="rpmB"
                     /locus_tag="RH057_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HDV3128225.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            25950..26117
                     /gene="rpmG"
                     /locus_tag="RH057_001848"
     CDS             25950..26117
                     /gene="rpmG"
                     /locus_tag="RH057_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HDV3128226.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            26215..27024
                     /gene="mutM"
                     /locus_tag="RH057_001849"
     CDS             26215..27024
                     /gene="mutM"
                     /locus_tag="RH057_001849"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HDV3128227.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(27063..27542)
                     /gene="coaD"
                     /locus_tag="RH057_001850"
     CDS             complement(27063..27542)
                     /gene="coaD"
                     /locus_tag="RH057_001850"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HDV3128228.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(27550..28827)
                     /gene="waaA"
                     /locus_tag="RH057_001851"
     CDS             complement(27550..28827)
                     /gene="waaA"
                     /locus_tag="RH057_001851"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HDV3128229.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDADYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            29240..30298
                     /gene="rfaQ"
                     /locus_tag="RH057_001852"
     CDS             29240..30298
                     /gene="rfaQ"
                     /locus_tag="RH057_001852"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HDV3128230.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
     gene            30295..31419
                     /locus_tag="RH057_001853"
     CDS             30295..31419
                     /locus_tag="RH057_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HDV3128231.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFELIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGVDRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            31412..32209
                     /gene="rfaP"
                     /locus_tag="RH057_001854"
     CDS             31412..32209
                     /gene="rfaP"
                     /locus_tag="RH057_001854"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HDV3128232.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYSADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPRRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
BASE COUNT         7628 a         8013 c         8673 g         7974 t
ORIGIN      
        1 gcagtctgat tattggtgaa gtttatagtc agtttttttc gccagcggtt aaagaaatag
       61 aatagtcgta taaaaaaggg tgttctgggg cgagtaaaac aacatcttca ccttaatggc
      121 gatacagttg gctgagagtc agcagggggg caaatcacct gcaaataaac acggcgcata
      181 ctggcgtgaa tatcgcggat gcgttcgagg acagaagaac cgacaggcgg attcttcagc
      241 cgctcatcgt ccatcacaaa accttagacc aggtattcct gaagccgatt gaatattgag
      301 cagtagaaaa tatctggatt gacagtatta caaggggttg gagaacgtca ggagaggcat
      361 tttggcggaa gatcacagga gtcgaacctg cccgggaccg ctggcggccc caactggatt
      421 tgaagtccag ccgcctcacc ggagacgacg atcttccgcg cctcgattgc tacatggagg
      481 cggggcgcat tatagctact tccttgagtt tctacatccc ccagatcagt tttttccgcc
      541 taatacacgc agcgtattat cacgaaagca ttatagttca tataaaaccc atattttaca
      601 atgatatatg agcggaatta acgctaccac gatcacataa aacaagaatc ataaattaat
      661 aaccagatat cggaatattc gctctcacag ggatggttta caaaatgcgt tatcggtctc
      721 agcaccccta tagcatcaag gaaaagcaga tgaagagtga agtgttgtcc gttaaagaga
      781 aaattggtta tggcatggga gacgccgcca gccacattat tttcgataac gtaatgttat
      841 atatgatgtt cttttatacc gatatttttg gcattcctgc cggatttgtc ggaaccatgt
      901 ttttggtcgc tcgtgcactg gatgcgattt ccgatccttg catggggttg ttggccgatc
      961 gaacgcgctc tcgctggggt aaatttcgtc cgtgggtact gtttggcgca ctgccattcg
     1021 ggatcgtctg tgtactggcc tatagcacgc cagatctcag tatgaacggc aaaatgatct
     1081 atgcagcaat tacttacacc ctacttacct tactttatac cgtcgtcaat atcccttact
     1141 gcgcattggg tggtgtaatc accaatgacc cgactcagcg tatctcgctg caatcctggc
     1201 gttttgtgct ggcgaccgcg ggaggcatgc tttctactgt tctgatgatg ccactggtta
     1261 atttaattgg cggtgataat aaaccactcg gtttccaggg cggtatcgcg gtcctttccg
     1321 tggtggcatt catgatgctg gcattttgtt tcttcaccac taaagaacgc gttgaagcac
     1381 cacctacaac aacgtctatg cgggaagatt tacgtgatat ctggcaaaac gaccagtggc
     1441 ggattgtcgg tttactaacc attttcaata tcctggcggt gtgcgtacgc ggtggggcga
     1501 tgatgtatta cgtcacatgg attttgggca cgccggaagt gtttgtcgct tttctcacca
     1561 cttattgcgt gggtaacctg attggttccg cactggcaaa acctctgacc gactggaaat
     1621 gtaaagtcac tatcttctgg tggacgaacg ccctgctggc agtgattagc ctcgcgatgt
     1681 tctttgttcc catgcaggcc agcatcacta tgtttgtctt catcttcgtg attggtgtgt
     1741 tgcatcaact ggtgacacct atccagtggg taatgatgtc cgataccgtc gactacggcg
     1801 agtggtgcaa tggtaaacgc ctgaccggga tcagttttgc tggcacgctg tttgtgctca
     1861 aactggggtt ggccttcggc ggcgctctta tcggctggat gctggcttat ggcggatatg
     1921 atgcggcaga aaaagcgcag aacagcgcca cgattagcat cattattgcg ctattcacga
     1981 ttgttccggc gatctgttat ttgctgagcg cgattatcgc taaacgctac tactcactca
     2041 cgacgcacaa tctgaaaacc gttatggaac agctggctca gggtaaacgc cgttgccagc
     2101 aacaattcac ctctcaagaa gtgcagaact aaggaacggc aatgaaaatt agcgatggaa
     2161 actggttgat tcaacctggc ctcaatttga ttcacccgct tcaggtgttc gaggttgaac
     2221 agcaggataa tgaaatggtg gtctatgctg ccccccgtga tgtgcgtgaa cgtacctggc
     2281 agcttgatac gcctttattt acgttgcgct ttttctcccc acaggaaggt attgtcggtg
     2341 tgcggattga gcattttcag ggggcgctga ataacggtcc tcattatccg ctcaatattt
     2401 tgcaggacgt gaaggtcaca atcgaaaaca cagaacgtta tgctgagttt aaaagtggca
     2461 acttaagcgc gcgtgtcagc aaaggtgagt tctggtcact ggattttctg agcaacggcg
     2521 aacgtattac cggtagtcag gtgaaaaata atggctacgt gcaggacacg aataatcaac
     2581 gcaattatat gtttgagcgg cttgatcttg gcgttggcga aacagtttac ggtctgggag
     2641 agcgctttac tgccctggtg cgcaatggcc agacggtaga gacctggaac cgggacggcg
     2701 gcacaagtac tgaacaggcg tataaaaata tcccgttcta catgactaac cgtggttatg
     2761 gggtactggt caatcatccc cagtgtgtct cttttgaagt gggatcggag aaagtctcca
     2821 aagtgcagtt cagcgttgag agtgaatatc tcgaatactt tgttatcgac ggcccgacgc
     2881 cgaaagcggt acttgatcgt tatacccgct ttactggtcg tccggcgctg ccgcccgcgt
     2941 ggtccttcgg cctgtggcta accacttcat ttaccaccaa ctacgacgaa gcgacggtaa
     3001 acagctttat cgatggtatg gcggaacgca atctgccgct gcatgttttc cactttgact
     3061 gtttctggat gaaagccttc cagtggtgcg attttgagtg ggacccgctg actttccctg
     3121 acccggaagg gatgatccgc cgcctgaaag cgaaaggact gaaaatctgc gtctggatta
     3181 acccctatat cggtcaaaaa tcccccgtct ttaaagagtt acaagagaaa ggctatttac
     3241 tcaaacgccc ggacggttcg ctatggcagt gggataaatg gcagccaggt ctggcgattt
     3301 atgactttac caatccggat gcctgcaaat ggtacgccga caaactgaaa ggtctggtcg
     3361 cgatgggcgt tgattgcttt aagaccgact ttggcgaacg tatcccaact gatgttcagt
     3421 ggtttgacgg ttccgatccg cagaaaatgc ataaccatta tgcgtacatc tacaacgaac
     3481 tggtgtggaa cgtgctcaag gacaccgttg gtgaggaaga agctgtcttg tttgcccgct
     3541 cggcctccgt cggtgcgcag aaattcccgg tacactgggg tggcgattgt tacgctaact
     3601 acgaatcaat ggcggaaagc ctgcgcggtg gtttgtctat tggcctttca ggttttggct
     3661 tctggagcca cgatatcggc ggctttgaaa ataccgctcc ggcgcacgtt tacaaacgct
     3721 ggtgcgcgtt tggtttgctc tccagccata gccgtttaca cggtagcaaa tcttatcgtg
     3781 tgccgtgggc ctacgatgat gagtcctgtg atgtggtgcg cttcttcacg caactgaaat
     3841 gccgcatgat gccgtatctg tatcgtgaag ctgcgcgtgc gaacgcgcgg ggtacgccga
     3901 tgatgcgggc catgatgatg gagttcccgg acgatccggc ttgtgattac cttgaccgtc
     3961 aatacatgtt aggcgacaac gtgatggttg cgccggtgtt cactgaagcg ggcgatgtgc
     4021 agttctacct gccggaaggt cgctggacac acctgtggca caacgatgag ctcgacggta
     4081 gtcgctggca taaacagcag cacagcttcc tgagtctgcc catttatgtg cgtgataaca
     4141 ccctactggc gctgggcaac aacgatcaac gtcccgatta cgtgtggcac gaaggcacgg
     4201 cattccacct ctttaatctg caagacgggc atgaagccgt ctgtgaagtg cccgctactg
     4261 acggatcggt gatctttact ttaaaagcag cacgtactgg caacacgatt actgtgactg
     4321 gtgcgggcga ggcgaagaac tggacgctgt gcctgcgcaa tgttgtgaaa gtaaatggtc
     4381 tgcaagacgg ttcgcaggct gaaagtgagc aggggctggt ggtgaagcct caagggaatg
     4441 cgctgacaat tacgttgtaa tctattgatg gaatgccggg tacgtctgag tcgtacccgg
     4501 cgagattgat gtttaccgca ccggctccgt cgatgtaact tcaccgctgg aaacgatgcc
     4561 agcattcatg gtttgagtca cttgctgctt tgcggcgttc acggcatgca attgcccgct
     4621 aaccgtaggt ttcaacggga cattggcctg aatatcgcca ctggcggtaa gctgaatatt
     4681 gccgtcgcca gtcaacggta atttaggcca tccccactgt tgcaaaatat tcaccggcac
     4741 accgcgtcca ttcaggctga tatgtgtctg acgttgtggc gtttgtgaaa cactggcggt
     4801 ggcttccaga atgccttttt cagtaaatgc actcagttcg ctgatattca ccgtgctgct
     4861 gttggcggtc agcgccagcg acgggcgacg aacatcaaca cgattgaatg tcgcggcggc
     4921 ggcattcaga ttcgccgagc cactccagac gccccattta tgatcggtaa ccagcgtcag
     4981 gttggcaccg taaccatcga gcgcggtgag ctgccacggg aagtcagggt cgatatcaat
     5041 gatcagattg cggctggcgc taaatctctt cagttgcagg ctgtttaacc aaccgggtgt
     5101 cgtttccatc cacaactgtt gccagttttt cggcaaggta tattccagcc cggcaattgc
     5161 cgcatcatca aggatcaacg ttttcccgtc acgcagccag ttccctgacg ttctgaccat
     5221 acccccttcc cagcggctgg tgaactggcg cagcgctacg ccctgcgggg aaaattccgc
     5281 gtttataatc gggtcaaata aatgcagcga accataaatg aactcgctag cgttcatcga
     5341 cagtttgcct tcttgtgtct gccagtcatc tttactgaag gtcatgttgc gcaagctgag
     5401 atcgaggtcg gtcaccgccc agtccggacc ttgcaaacga gcatcgatca cttccaggcg
     5461 accaatctgc aacgacggga cagagcgtaa tggcgcaaag aagtcggtta gcgatttttc
     5521 gctttgtaga cggatatccg ccatgcgcag attttccact tgccagctgc cgtcggcgtt
     5581 acgctgcgcg tttccggtta atgtcccgcg ggcgatgtcg gcacccaggt tagtcagcgt
     5641 aacgcgatcg ttatcaatac tgccttcaat cagtacattg gtggcaggaa catcattgag
     5701 cgaaagcgat ccggcactaa actgaatctg cgccttcgta cccagcactt taccggcttc
     5761 tggtgaccac ggaaccacgc cgccatttac ccgctgcgcg ctcagtttcc attcgctatt
     5821 cgggctatta aacgccatat cacgcagttg cagacgatcg gctttgaacg gtagcggcgc
     5881 ggtctggtcg gtgagattca gcgtcccgtt ttccagcagg atggtatcga catggcgtgg
     5941 ttcggtcagt tgccgactgc ttagcgcaat gtcgacactt tttgccacca gggtcgcggg
     6001 ctgaccatca cgaccaaacg tgacgttctc cagcacgata tgagatggcg cggaaaaacg
     6061 gtgatccatc gccccgaagg ccagatgata gtcgctattc tcggaaaccc atgcgctgat
     6121 atgttctgct ccccagcggg tttgcagaag aaaatagagg ccagcgatca ccactaacag
     6181 agcgatgaga atgtagagaa gcagcttccc aataaatttc atggtcttcc atcccgtgaa
     6241 atgcacataa gggagttatg cacgatttac gcgcaatcct caaggcggga atggtgaaag
     6301 aggtgagact gcggcggtaa ttatcttacc gccgccagtg aattactgtt tttctggtgg
     6361 gaaaatcaga ttcagcacga tggcagtaat accgcccgcg gcgatcccgg aggagagcag
     6421 gtttttcagc cattcagggg caaactgcaa aatcagcggc tgctgagaca cgcccagacc
     6481 aaccgccagc gacagcgcga taatcagaat cgcccgacgg ttcagcggct cacgagaaac
     6541 gatacgcaca ccggaggcgg cgatggtgcc aaacattaca agcgttgcgc cgcccagaac
     6601 cggttctgga atgtgttgta caaaaccgct cactgccggg aacagaccca gcacgatcaa
     6661 catcagcgcg acgacaaaac cgacatagcg gctggcaaca ccagtcaact ggatcactcc
     6721 gttgttttgc ccgaagcagg agttcgggaa ggtgttaaac accgccgaaa caaacgagtt
     6781 caggccgttt gccagcacgc cgcctttcag gcgtttcatg tacagcggac cggacactgg
     6841 ctgttcggaa acgtcagagg tcgccgtgat atcgccaatg gtttccagcg aagtgatcat
     6901 aaagaccagc atcagcggca gcagcagact ccattcaatg ccaagaccgt aatagagcgg
     6961 cgttggcacc ataatcagtt cttgcgtcat cggttcgttg ctttctggca acatgcccat
     7021 aaaccacgcc agcgcatatc cggccgccat cgcaatcacc agtgaggcca cgcgtaagta
     7081 agggttacgt tgacggttaa gcaggataat taaggctaag accacgcctg ccagcagcag
     7141 atttttcggt gcgccgaagg tgttatcgct catggctgcg taaccgccgc caatggacgt
     7201 taacccaacc tgaattagcg acaggccgat aatcatcacc acaacgccag aaaccagcgg
     7261 cgtaataatg cggcgcgcca gatgcagaac gcgggagatc accatctcgg tgcaacttgc
     7321 cagcatcaac gtgccgaaca aagccgccat catggtagga acatcagcac caccggtttt
     7381 cagcgcggta ccgcccataa tcagcggggc aacaaagttg aagctggtgc cctgaataga
     7441 caacagcccg gagccaaccg gaccccaggc cttaatttga ataatcgatg ccacaccgga
     7501 ggcaaacagc gacatactaa taatgtgttg cgtgtcttgt gccggtaaac ccagcgcctg
     7561 gcagattaat agcgctggcg tgatcaccgc aacgaacatc gccagcagat gctgacaggc
     7621 ggcaaacagg gtttgaggaa gcggcggacg atcttcaaga cggtaaatca gttcgctgtt
     7681 ttgagtctgc gcaaccggtt gcgcattttc tgactcgagg gtggaaacag acatcgaaaa
     7741 caatcccgtg gtagcaaagc gggcatttta gctgaccgaa agagaaaagc aaacgtttgc
     7801 cacatcctca tctaaatatt taaatgagat aaatgcactt ttttatataa cttttggtat
     7861 ttttctgctt aaaatccatg ccatttgttc attaacagct gagaatttga cccaggaaag
     7921 cataactcga tacccgtcat agttcatgaa gttgccactg cgcgatgaag tatgacgagt
     7981 atgaaagagt gatgcggata caaaggagta actatgtttc atctcgatac tttagcaacg
     8041 cttgttgccg caacgctgac gttgctgctc gggcgtaagt tggtccattc cgtctccttt
     8101 ttgaagaaat acaccatacc ggaacctgtt gcgggtggtt tgttggtggc gctggcgcta
     8161 ctagtactga aaaaaagcat gggctgggaa gtcaactttg atatgtccct gcgcgatccg
     8221 ttaatgctgg ctttcttcgc caccattggc ctgaacgcca acattgccag tttgcgtgcc
     8281 ggtgggcgtg tggttggcat cttcttgatt gtggttgttg gtctgttggt gatgcaaaat
     8341 gccattggca ttggtatggc tagcctgtta gggcttgatc cgctgatggg gctgttggcc
     8401 ggttctatta cgctttccgg cggtcacggt acgggcgctg cgtggagtaa attgttcatt
     8461 gaacgttatg gcttcaccaa tgcgacggaa gtggcgatgg cctgtgcaac gttcggtctg
     8521 gtgctgggcg gcttgattgg cggtccggtg gcgcgctatc tggtgaaaca ctccaccacg
     8581 ccgaacggta ttccggatga ccaggaagtc ccgacggcgt ttgaaaagcc ggatgtggga
     8641 cgcatgatca cctcgctggt gctgattgaa actatcgcgc tgattgctat ctgcctgacg
     8701 gtggggaaaa ttgttgcgca acttttggct ggcactgctt ttgaactgcc gaccttcgtc
     8761 tgtgtactgt ttgttggcgt gattctgagc aacggtctgt caatgatggg cttttaccgc
     8821 gtctttgagc gtgcggtatc cgtgctgggt aacgtaagcc tgtcgttgtt cctggcgatg
     8881 gcgctgatgg ggctgaaact gtgggagctg gcttcgctgg cgctgccgat gctggcgatt
     8941 ctggtggtac agaccatctt catggcgttg tatgccatct tcgttacctg gcgcatgatg
     9001 ggcaaaaact acgatgcggc agtgctggct gcgggtcact gtggttttgg cctcggtgca
     9061 acgccaacgg caatcgccaa catgcaggcg atcactgaac gctttggccc gtcgcacatg
     9121 gcgtttttgg tggtgccgat ggtcggtgcg ttctttatcg atatcgtcaa tgcgctggtg
     9181 attaagctgt atttaatgtt gccgattttt gccggttaaa aatgaaaaag cgtcggaagg
     9241 aaccggaaac gctacgtgaa cactgtcgcc atatttttgg tgatgagccg ccagtgctct
     9301 gtgtatggga aacggaattt gattacgcag atgccgaact caaggcgctg gcggctaaag
     9361 agtggcagca gatcagcgaa cgggatttga gtgcttacta tgtactcaat ctcgtttata
     9421 acgaaccgat gcaaatagag ttgtttcgct atctgtttcc gctctgcctg gcgcaatggc
     9481 atgaaaccgt tttagctggc ggttatggtg atcacttcga agaatcctta atgaaggcat
     9541 tgtgtcgacc ctatctctgg caggagatga tgaatgcttc ccaacgccag caggtacggc
     9601 aatttttact ggacaccgcg ttgcagcgta tggataacga gcgtggtttc aataacgttc
     9661 tttgctggct ggcggtgttc aatactctgg gtggggccgc gccgttaatt cgttctctct
     9721 ggtcgcgctg gtgggcgctg gatacgccag gaaaagcggt gtgtgctatt cagtatgccg
     9781 cgcatctaat ttatcccata gaagccaatc cgttatggtc gcaagaatgg ataggctggg
     9841 ggcatccatt aggccataaa gatggctggt cctctgataa tcgcgctttt cttcgccaaa
     9901 tgttaacacc tgaaatgatt gtggctggcg tacaggcagc ggctgaaatt ctgcgtggtg
     9961 aaccggaagg cgcaatggcg gcgcgaattg cacaggatgc gtatgaggca atggatatcc
    10021 tcactatcca gattgaagat ttactgcgtg acttgtcttg tgatgagtct ggtcatgcgt
    10081 tggagtaacg ttccatctcc ggcatccagc gttctatcag ggcttttgct tgttgtgggt
    10141 aacgttcgtg aatatgtcgc gccaggcgct gaacttccgg gatcatcgcc tgatcgcgca
    10201 gtaaatccgc cactttaaac tcagcattac ccgtctgacg cgtacctaac agttcacccg
    10261 gtccgcgaat ctctaagtct ttctgtgcaa tcacaaaacc gtcgttgctg tcgcgcagca
    10321 cttgcagccg aatttgcgcg gttttggaga gcggcgtttt gtagagcagc acgcagtgag
    10381 aagccaccgc accacgacct acacgcccgc gaagctggtg taactgcgcc agacccagac
    10441 gctccgggtt ttcgataatc atcagactgg cgttaggcac atcaacgccg acttcaataa
    10501 cggttgtggc aaccagcagg tgtagctcac cttgtttaaa cgacgccatc accgcctgtt
    10561 tctcggcagg tttcatccgc ccgtgtacca ggccaacgtt caactctggt agcgccagtt
    10621 tcaactcttc ccaggtagct tccgccgcct gcgcttccag caattccgac tcttcaatca
    10681 acgtacaaac ccagtacgcc tgacgacctt cagttatgca ggcgtggcgc acgcggtcaa
    10741 tgatgtcggt acggcgggta tcaggaatag cgaccgtagt cactggcgtg cggcctggcg
    10801 gcagctcatc tatcaccgag gtatcgagat cggcatacgc cgtcattgcc agcgtgcggg
    10861 ggatcggcgt ggcggtcatg atcaactgat gcggatggaa gccttgctgc tggcctttct
    10921 cccataacgc cagacgctga tgcacgccaa agcgatgttg ttcgtcgata atcaccagcg
    10981 ccagaccgtt aaactgcacc tgttcctgga agatggcgtg cgtaccaaca atcatctgca
    11041 cctgaccgct ggcgatggct tcctgctgag acagtcgcgc tttgcctttc tgcttaccgg
    11101 cgagccagcc cacttcgata ccgagcggtt caaaccagtt gcggaagtta ttggcgtgct
    11161 gttcggcgag taattcggtt ggtgccatca atgccacctg tttgccgtgg gcaatcgcac
    11221 gcagcgcggc gagggcggcg accagcgttt taccggaacc tacatcgccc tgtaccaggc
    11281 gcatcatcgg cacatccagc gccatatcgc gctcgatctc cgccactacg cgtgcctgtg
    11341 cgcccgttgg cttgaacggt aaggctgcga ggagtttatt tttcagcgca tcattggcgc
    11401 tcagcggctg ggcatgaaaa cgctgtgctc cggcacgtaa ggctaacatg ctgaggttgt
    11461 gcgccagcag ttcttccaga atcagacgac gttgcgccgg atgctgcccg gtttccagat
    11521 cgctaagctg tagcgtcggt ggcgggcggt gcaaagtgcg caacgcttcc ggtagcgtca
    11581 tcattccttg tgacagttcc ggcggcagga gttcttcaat ggcgcaggtg tcgagcagat
    11641 ccagcgcctg gtcggttaat ttacgcagcg tggcctgctt tacgccttcc gttgttggat
    11701 aaaccggcgt gagcgtttcc tgtaattctg gcgtgctgag atcgccctgc acgcggtatt
    11761 ccgggtggat catctccgca ccatatttac cgcgctttgc ttcgccataa gccagtacac
    11821 ggcggcccgt cgccaggcta tttttcattg ccgcgctgaa attgaaaaag cgcatggtga
    11881 ggatgccgga accgtcgctg atctggcagg tcatcatccg ccgaccgccg aaggagatat
    11941 tgcagttcag cacttcgcct tccaccgtgg cataaacgcc cggcagtagt tctccgatgg
    12001 ggtagagatg ggtgcgatct tcgtagcgca ggggaaggtg taagagtaga tcctgcacgg
    12061 tatgcaggtt gattttcgcc agtttgttac taagtgctgc gccaacgccc gttagggaac
    12121 tgagtgggac agcatctaac aggcgacctt tcatggcact taccctgcag cctgcatagt
    12181 agcccaccag tcggcatcag cttcgatctc gccttgctga ttgacgtggg gataaggcag
    12241 gcctttgcgt tttgcgactt tcgccagcac cggatagccg ccttcaaaca acaggcgttg
    12301 ttgctctgct tccggcaaca tgctgttttc acgcaggtac atgcctgcat tttgccgctg
    12361 acgctgggct tcgtaaagaa tgagggctga ggcaacggaa acattcagcg actgcaccat
    12421 gccgatcatc ggaatgatga tgtcctgatc cgccagggcc aatgcttcct gcgtgatgcc
    12481 cgttttctcc tgtcccatca aaatgcaggt cgggcgagtg taatcaattt cgcggaaatc
    12541 gacagcgtta tcagaaagat gggttgccag aatctgcatg ccccggcctt tgagatgagc
    12601 gacggcatcg ccaatggtgc ggtgtgtttt cacctgtacc cagctgttac tacccgccgc
    12661 tgccgaagcc atggtgcgca tgcggctacc aggccagacg gcgtgaactt catgtacgcc
    12721 aacggcatct gcggtacgaa taatcgcaga aacgttatga ggtttgtgga cctgctccat
    12781 gcagacggtc agatcaggct gccgcctggc gagcatttcg cagatgcgtg cataacgtgt
    12841 tgggttcata aaacattaat ttcggtttcg ggtgacttta atcacgtctg gcatcacgcg
    12901 gattttgcgc atgatattcg ccagatgcac acggtcacgg gcggtcagac gaataaaggc
    12961 gctgtagacg cgaccatctt tctcttccgt attcaaactt tgaatattcg aagtcgtggt
    13021 gttaattgcc gccgtcaggt ttgccagcgc gccctgatga ttgaacatct ccaccttgat
    13081 ttcggtgatg aactcctgcg ccgtctcttt atcccattcc acagccataa acttctctgg
    13141 ctctttctgg tagccacgga tgttacggca ggattcatgg tggatcacca gacctttacc
    13201 ggggctgacg tgggcgataa tcgggtcgcc aggaataggg cggcagcatt tcgcaaaggt
    13261 gatcagcacg ccatcggcac ctttaatggg cagatgtccg tggctttggg ttgccggtgg
    13321 aatggaggcg tccccatgtt gcagattttt cgcgaccacc acgctcattg cgttaccaag
    13381 tccgatttct gccagcagat cgtcaagcgt tgccagcttc atgcgatcca gctcgcgctg
    13441 aatattttcc tgcgggattt cattgagctt acggctacca cccaaagcat ggttgagcag
    13501 acgacggccc aggcttacag aatcatcacg cttgaggttt ttcagcaact gacgaatttt
    13561 ggcgcgcgct tttgagctaa cgacaaagtt cagccacgcg gcattcgggc gagcgcccgg
    13621 tgcggtaatg atttcaacgg tttgaccgct ggtaagcggc tgcgacagcg ggtaaggctg
    13681 gcggtcaacg cgtgcgccca cgcaggcatg accgatatcg gtatgcactg cataagcgaa
    13741 gtcgacgggc gttgcaccgg caggcagctc gacaattcgc ccttccggtg tgaaaacgta
    13801 aatctcatcc gggaagagat cggatttaac gctctcgata aattcaaacg aactaccggc
    13861 gctctgttgc agctccagca ggctttgcat ccagcgctgg gcgcggattt gtgcggtagt
    13921 actggtttcg ccgtgctctt tataagccca gtgcgcggca acacccatct ccgccatctg
    13981 atccatatct tcggtacgga tctggacctc aaccggcacg ccgtgcgggc caatcatcga
    14041 ggtgtgcaac gactgatagc cgttcgcttt aggaatggcg atatagtctt tcacgcgacc
    14101 cggacgcggc ttgtacaggc tatgcatttg cccgagcacg cgataacagg tgtcagaatc
    14161 attgacgatc acgcggaaag cgtagatgtc catgattgag tgaaaacgct gctctttgag
    14221 caccattttg cagtaaatcg aataaagatg cttttcgcga ccactgacgc ggcacggtat
    14281 tcccgcttcc tgcaaacgcc cttcgatttc agagaggatt ttttggatca tctctttacg
    14341 gttgccgcgc gcggctttca ccacttcttt aattacgcgg taacgattgg gatacagcgc
    14401 ctcaaaaccc agctcttcga gttcggtttt aatgtggtgg atacctaaac ggtgcgccag
    14461 cgggctataa atttcgagag tttcacgggc gatgcggcga cgtttgtccg ggcgaagtga
    14521 gcccagcgtg cgcatgttgt gggtacggtc ggcaagtttg atgagaatga cgcggatatc
    14581 ctgcaccatc gccataatca tcttgcgaaa gttttcggcc tgcgcctctt tcttatcgcg
    14641 gaacttgagc ttatcaagtt tcgacacccc ctctaccagc tcggcgacgc ttttaccaaa
    14701 aagctgttcc atgtcctggt aggtggcggg agtatcttca atcacgtcat gcagcagcgc
    14761 cgccatcagc gtttcatagt cgagtttcat ctcggccaga atgcaggcaa ccgctaccgg
    14821 gtgcgtgata tagggttcac cgcttgaacg tgtttgcccc tcgtgagcat cacgtgcaac
    14881 gagatacgcc tgccgcagac gcttgatttg gtcttccggc aggtaggttt gaatcagttg
    14941 attcaggctt tcaaacagat acaagggcga cccgctttgt gattaacgac gaccttcagc
    15001 aatagcggta acggcttgta attcagcggc ttcctgctct tgctgttcct ggcgttcgcg
    15061 aacgtcgagg atctggttgt tgatcagacc ttcttcgatt tcgcgcagcg cgattacagt
    15121 ggttttatcg ttttcttccg gtaccagcgg atcctttccg cctacctgca tctgacgagc
    15181 gcgacgcgcg gcgaccagta ccaggtcaaa acggttacca attttctcta cagcgtcctg
    15241 aacagttacg cgtgccatac ttaaaaagct ccacaggtga agaaatgact gggcatgata
    15301 ctgaaatcag gttcagtctg ccaacaattt gctgattaaa gcgtcatgac gctgcttttg
    15361 gcggctcatg cgcagacgtt cggcgcgaat aatggtcttc aaatcggtca acgcggtatc
    15421 gaagtcatca ttcacaatca gataatcata ttcggcgtaa tggctcattt ctgcaacagc
    15481 ttgcgccata cgctttgcaa tgacctcttc gctgtcctga ccgcgaccgc gtagacggcg
    15541 gtccagttca attttggacg gcggtaaaat aaagatactc cgcgcgtgcg gcattttctg
    15601 gcgaatttgc tgcgcgccct gccagtcgat atcgagaaaa acatcgacac cggtcgccag
    15661 tacttgctca atggcctcac gcgaagtgcc atagtaatta ccaaaaactt ctgcgtgttc
    15721 gaggaacgca tctctgctaa tcatttcttt aaattcatca tgattaacaa agaaataatg
    15781 ttcaccgtgg acttcaccag gacgcggttg gcgtgtggtg tgtgaaacag aaacctgggt
    15841 gtcatacaac ggttgggttt ttaataaagc ctgaatcagg ctggatttac ccgcgccact
    15901 gggggcagaa acaatataaa gcgtgccttg agccatgagt atctttcgta tgtgattagc
    15961 gaaataaagc ctacatacga gcttattata cacggcgcgg ccatgtgacg tagcccttgc
    16021 tcacactttt tgcgcaaagt tgttgcgttt tgcgtagctg tttccgcatt tgcctgtgat
    16081 ttactgcaat aaagacgatt ctcaggaagc gtcctcgcag actccgtttt tgccactcgg
    16141 ctttgccttc accacgttgt atatctgcca gcctgactac agcggaggtg aagatgaaag
    16201 tatggatggc gatattaata agtatcttgt gctggcaatc atctgtgtgg gcggtctgtc
    16261 cggcctggtc gccagccaga gcacaggaag aaatttcccg cctgcaacag caaataaaac
    16321 agtgggacga tgactactgg aaggaaggaa aaagtgaggt ggaagacggt gtttacgatc
    16381 agttaagcgc ccgtcttacg cagtggcaac gctgctttgg gagcgagccc cgcgacgtga
    16441 tgatgccgcc tttaaatggt gcggttatgc atccggttgc ccatacgggt gtacgcaaaa
    16501 tggtggataa aaacgcatta agtctgtgga tgcgagaacg tagcgatctt tgggtgcagc
    16561 caaaagttga tggcgttgcg gtaaccctgg tttatcggga cgggaaactg aacaaagcaa
    16621 tcagtcgcgg taatggcctg aaaggcgagg actggacgca gaaagttagc ttaatttccg
    16681 ctgtgccgca aaccgttagc gggcctttag ccaacagtac gcttcagggg gaaatatttc
    16741 tccagcgcga ggggcatatc caacaacaaa tggggggaat aaatgcccgc gcaaaagttg
    16801 ctggcctgat gatgcgccag gacgatagcg acacgctgaa ttctctgggc gtttttgtct
    16861 gggcatggcc ggatggaccg cagttaatga ccgatcgttt aaaggagctg gctaccgcag
    16921 gttttactct aacgcagagg tatacccgtg cggttaaaaa tgctgatgag gttgcgcgcg
    16981 tacgcaatga gtggtggaaa gcgaaattac ccttcgtcac cgatggcgtg gttgtgcgag
    17041 gggcgaaaga acccgaatcc cgccattggc taccaggcca ggcagaatgg ctggtggcct
    17101 ggaaatatca acctgtagct caggttgccg aagtgaaggc gattcagttt gcggtgggta
    17161 agagcggtaa aatatcggtg gttgcgtcac tcgcacctgt catgctggat gataaaaaag
    17221 tccagcgggt gaatattggt tccgtcaggc gctgggagga gtgggatatt gcgcctggtg
    17281 atcagatcct cgtcagcctt gccggtcagg gtattcctcg cattgatgat gtggtctggc
    17341 gcggtgcaga acgtacaaaa ccgacaccac cagaaaaccg ctttaactcg ttgacctgct
    17401 actttgcttc tgatgtttgt caggaacagt tcatttcacg cttagtctgg ctggggtcaa
    17461 aacaggttct ggggctggat ggcattggtg aggccggctg gcgcgcgctg catcagactc
    17521 atcgctttga gcatatcttt tcctggcttt tattaacgcc agagcaatta cagaacacgc
    17581 cggggatcgc gaaaagtaaa agtgcgcagc tatggcatca gtttaatctg gctcgtaagc
    17641 agccttttac tcgctgggtg atggcaatgg gaataccgct aacccgggcg gcgcttaatg
    17701 ccagtgatga acggtcctgg tcgcaacttt tatttagcac ggagcagttc tggcagcagc
    17761 tgccggggac tggatcgggg cgcgccagac aggttattga atggaaggaa aatgcgcaaa
    17821 tcaagaagct gggcagttgg ctggctgccc agcagatcac aggttttgaa ccttagtgtc
    17881 cttcgtggct gtagtaaaag accggtaatc ccagcttaag gcgtagcgcc agtaagcggg
    17941 caaaaaagcc gaataccagg gtggagataa ttaccacatc atggttagaa acatagtgtt
    18001 gcagcgcgat gtacagcacg gcggaggcaa atgagacacc ggcatacaac tctttctgga
    18061 ataccagtgg gatacgttta cagaacatat cgcgcaaaac gccgccaaac acgccggtgg
    18121 ttaccgccgc gacaacggca ataattgggc cgtgccccat atccagtgca acctgtgcgc
    18181 cgatgataga aaagaccacc agtccaagcg catcgagcac cagaaacact ttgcgcaggt
    18241 aaggcattac aggagcaacg atagtggtga gtactgcggc ggtagcgacg ataatcacat
    18301 attccgggtg tttgacccag ccgagcggat agtggcccag cagaatatcg cgcactgacc
    18361 ctccgccaat tgcggtggcg gtcgcaataa taattacgcc aaatgtatcc atgcggcgtc
    18421 gcccggccgc cagagccccc gtcatggctt cggcagtaat gccaaccaaa tacaaaatgt
    18481 gtaacagcat ggttctctcc ctaaaaatct gtctggcagg gtagcgattt gtgcgtattg
    18541 tcacgattga gattttttaa ggtgagagga aattgataac ttgtagttat gtgaaatcgc
    18601 tattttctgt agcgtagatg cattcttctg acttcttgtc tgagaaaatg cataagaaaa
    18661 aataggttaa aattgagggg tggaaaagaa aggtgcaatg tttagccatt gtgttcaccg
    18721 cacctggact ttggcttacg catcgggtat ctcaatcaac aaaaagagtt tttagtttct
    18781 gctatttctt ctctgtaatt tttacactgt tttccaactg cttgatgctg acctgcgggg
    18841 tcggcaactc ctcaggcatg gttccgccaa gttcctgaat ggtttgcctc accttgctac
    18901 ccacgtcaaa gtgtgtggta ttagcttgct gttttgaatt cacgccatcc cgcttgagtt
    18961 tttcttctgt ttgggtagct cgaaagagat tagccgccag ttctgttgaa cccatatgat
    19021 ccaggatctt ctgactcttt ttcagcccct tcagttggtg gatggctttc tgatctaatc
    19081 caccatacag cccctggtaa ccatgattct gaaatatggc gaaatcagta gctgtcgcta
    19141 ccccagcttg ctgtgctgcc tcaaccaatt gtttgttatg ttctttcaac tcatttctca
    19201 gaaacagacg cttttcgtct tcacgaagtt gcttaaatgc ttcatcgtca gcaagctcct
    19261 gccgtcgggt ctggatagca aaataagtct gccctgccgc aatgaccggt ttcgcagggt
    19321 cgccgttttg tactaccagg taacaggcat agcgggagag atgaacatct tcaagttctc
    19381 gttgagcccc tgaacctaat acaaccattt tggtggtttc aacgaaatgg tcagatgcag
    19441 cttgattgct ggcttcgcac gcttgagtcg cgcgggccag taccttttga aaattgcgcc
    19501 agtcccgata atccagtaac ggtgccaatt ctctagctga ccaaaattct tgcccttcag
    19561 tgccataatg ccgtatttct tcaaaaggtt gatgatgttc gttcattgcc cactccatgt
    19621 gatatcgatt gcaaagaaag taaatctgta actgtattta tatacagttg tgagtatctc
    19681 acgttctcag tgtgctgggc aaaaaaacat cgcaaaatct ggagcaggca cgcaaaacag
    19741 aattgaagtt gcggggaaga aaaatgccgc tctgtttgag tgggcggcat ttgtgaaggc
    19801 tgaaaagtaa gaattagtgt tacgagttac tcaatattct gaatctgctc gcgcatctgc
    19861 tcaatcaaca ctttcagctc gatggcggag tttgtcactt cggcattgat agatttcgac
    19921 gcaagagtgt tcgactcgcg gttgaattcc tgcatcataa agtccagacg acgaccaacg
    19981 gcttcttttt tcttcagaat gttgtaggtc tctttgacat gcgcttccag gcggtccagt
    20041 tcttcggcaa cgtcaattcg ttgtgccagc agaaccagct cctgctccag acggttgttt
    20101 tccaactgaa cctgagcatc ttccagcttc gcgaccagac gctcacgctg ccattgcagg
    20161 atttccggca tatgggcgcg gactttgacc acttcggcgg tgacgccttc cagacgctgc
    20221 tcgatcaatg ctttcagcgc ctgaccttcg gtttcgcgcg cgacgataaa gtcgtccagc
    20281 gtaccatcca acgccgcgag aatttcagcg gcaatcgcgt cgagatcctg ctcctgggct
    20341 gccatcacgc ccggccagcg tagaatatca accgggttga tttccccttc gtcactctgc
    20401 atttttaccc agttcgcggc agttaccagc tgtttagcca gtttttcatt gaggatcagc
    20461 tcaccttgtg cgctaacatc tggctcatag cgcagggtac attccacttt accgcgcgtc
    20521 aggcgagaac gaatacgctc gcgaacgaca ggttcaaggc tacggaactg ctccggcaga
    20581 cgaaagtaag tttccagata acgctggttt accgagcgca tttcccaggt tgcgctcccc
    20641 cattcaccct tgatttcacg ccgggcgtag gcggtcatac tgcggatcat agacgttcct
    20701 gtttataaaa ggagaggtgg aaggattata gccatcgatg ccttgtcagg ataggaataa
    20761 ccgccggaag tccgtataat gcgcagccac atttgtttca agccggagat ttcaatatgc
    20821 gtccagcagg ccgtagcaat aatcaggtgc gtcccgttac cctgactcgt aactatacaa
    20881 aacatgcaga aggctcggtg ctggtcgaat ttggcgatac caaagtgttg tgtaccgcct
    20941 ctattgaaga aggcgtgccg cgcttcctga aaggtcaggg ccagggctgg atcaccgcag
    21001 agtacggtat gctgccgcgt tctacccaca cccgtaacgc tcgtgaagcg gcgaaaggta
    21061 agcagggtgg acgcacaatg gaaatccagc gtctgatcgc ccgtgctctt cgcgcggcag
    21121 tagatttgaa agcgctgggt gagttcacca ttacgctgga ctgcgacgtg cttcaggctg
    21181 atggtggcac tcgtaccgcg tcgattacgg gtgcctgcgt ggcgctggca gatgcgctac
    21241 agaagctggt ggaaaacggc aagctgaaaa ccaacccgat gaaagggatg gtagccgcag
    21301 tttctgtcgg aattgtgaac ggcgaagcgg tttgcgatct ggaatacgtt gaagactctg
    21361 ccgcagagac cgacatgaac gtagtgatga ccgaagacgg gcgcatcatt gaagtgcagg
    21421 ggacggcaga aggcgaaccg ttcacccacg aagaattact caccttgttg gcgctggccc
    21481 gagggggaat cgaatccatt gtagcgacgc agaaggcggc gctggcaaac tgatttttaa
    21541 ggcgactgat gagtcgcctt ttttttgtct gtagaaaagt aagatgagga gcgaaggcat
    21601 gaaaccatat cagcgccagt ttattgaatt tgcgcttggc aagcaggtgt taaagtttgg
    21661 cgagtttacg ttgaagtccg ggcgcaaaag cccctatttc ttcaacgccg ggctgtttaa
    21721 taccgggcgc gatctggcac tgttaggccg tttttacgct gaagcgttgg tggattccgg
    21781 ccttgagttc gatctgctgt ttggccctgc ttacaaaggg atcccgattg ccaccactac
    21841 cgccgtggcg ctggcggagc atcatgacct tgacctgccg tactgcttta accgcaaaga
    21901 ggcaaaagac cacggtgaag gcggcaatct ggttggtagc gcgttacaag gacgcgtaat
    21961 gctggtagat gatgtgatca ccgccggaac ggcgattcgc gaatcgatgg agattattca
    22021 ggctaatggc gcgacgcttg ctggcgtgtt gatttcgctc gatcgtcagg aacgggggcg
    22081 cagcgagatt tcggcgattc aggaagttga gcgtgattac aactgcaaag tgatctctat
    22141 catcaccctg aaagacctga ttgcttacct ggaagagaag ccggaaatgg cggaacatct
    22201 ggcggcggtt aaggcctatc gcgaagagtt tggcgtttaa agaaactcgc cggatgaaaa
    22261 gtcatccggc gtcatattac tgcaactgcg cggcaattag cggccagcgg gcgtcaaaat
    22321 catccgtcgg gcggtattta aattcgctgc ggacaaaacg tgacagcata ccttcacaga
    22381 aggccaggat ctggcttgcc agcagggttt catcggtggt gtaaccttca ccctcacgca
    22441 ttctcttttc acgcaatacc tggcgcagct gcgcttcaat acgctcgaac agctggttga
    22501 tgcgcccttg caggcgatcc tgttcaaaca ttagcgcatg accagtgagg atgcgggtca
    22561 ggccaggatt acgctcacca aaaccgagaa gcagcaacac aatcagacgc aggcgcgctg
    22621 tggtgtcttt ctcatctttc agaatcaggt tgatgcgagt aatcaggcta tcttcgataa
    22681 actcaatcag gctatcgaac atgcgggtct tactggggaa gtggcgatac agtgccgctt
    22741 cggaaacgcc gacagaggcg gccagttttg ccgtcgtgat acgttggctt ccatcgctgg
    22801 attccagcat cagcgccaga gactgaagta tttcctcgcg acggttcctt ttcgcagttt
    22861 gtttttctgc catgttacaa aatacccctg aaaataagca cttgccaggc ggcacccacg
    22921 ctatgaccgc aaacgaaatg tttgcggcta tgttatgacg ttattcggat gcgtatgtgt
    22981 tactgacgac cagagtgacc aaagccgcct tcaccgcggt cggtggcgtc gaaatcttcc
    23041 accagattaa attcagcctg tactaccgga acaaaaatca tctgggcgat gcgttcgcca
    23101 ggttgaatgg tgaagctgtc ctgatcacgg ttccacacgg aaatcatcaa ctggccctga
    23161 tagtcagaat cgatcaatcc taccaggtta ccaagcacga taccgtgctt atgtcccaat
    23221 ccggagcgcg gcagcatcat tgccgccagt gaaggatcgg caatatgaat cgccagcccg
    23281 gtcggaacca gcgtagtgtc acccggagcc agttctacgg cgtcgtcgag acaggcacgc
    23341 aggtcaagtc cggcagagcc agaggtggca taagtcggga gcggaaattc cttcccaacg
    23401 cgcgggtcca gaatcttaac gtcgattttt ttcatcataa cgggtcacga tctcgtcgag
    23461 taataattgg ccaaggagct ctttgcgctc aagcggtaag actttatctc cgtcctgcca
    23521 gaaaaggtgt aatgcgttgt tgtcgctgtt aaatccttga gttggctggg aaacatcgtt
    23581 cgcgcagatc agatcaaggt ttttacggat acgtttttgc cgggcgtatt cttccacatt
    23641 atttgtttcg gcggcaaatc caacgacgta gggtcgatgg tcttttagtg cggcaacgcc
    23701 tgcgacgata tcggggtttt taaccatttt tattgttaat tcatcaccct gcgtggcctg
    23761 ctttttgatt ttctctgggg ccacggtagc tgcgcgataa tccgccacgg cggcgcagcc
    23821 gataaaaata ttttgctgct gtacagaagc attcacggcg gcttccattt ccagcgcggt
    23881 catcacatca acacgtttaa caaacggtgg cgtcggtagt gaaaccggac ctgataccag
    23941 cgtgacgttc gcgccacgac gggcagcggc ggcggcgata gcaaaaccca tcttgccgga
    24001 gctgtgatta gagatataac gcacgggatc gagcggttca cgcgtcgggc cggcggtaat
    24061 cataatgttc agatgtttca ggtcgttgac gggcgaaaaa tgcgctaccg ccatatccac
    24121 aatggttaac ggatcgagca ttcgcccagg accgatatca ccacaagcct gactgccact
    24181 gtctggcccc cagatgagca aaccacggga agcaagcacc tctaaattat gctgcgtggc
    24241 agcggcacgg tacatctgct ggttcatggc ggggagcacg gctacaggcg caggtgtagc
    24301 cagacaaatc gtcgatacca ggtcattcgc cattccggca gcaacacggg caatcaaatc
    24361 tgccgtggca ggggcgagaa tcactaaatc agcccattta cccagctcaa tatggcccat
    24421 agcggcttcg gctgccgggt ccagcagact gtcggaaacg ggataaccag aaaccgcctg
    24481 caagctaagt ggggtgataa aggcttttgc cgcttcggtc atggctacgc ggacgtcggc
    24541 cccgcgatcg cgcaaacgac gcaccagttc aggggtttta taggcagcaa taccgccgct
    24601 aacgccgaga acgatttttt taccggccag gctcatgatg atttttcctg ttgggtgaca
    24661 ccagaagttg gcgattttat cacaatactt ttgttgtcgt gccttcacct gagaatcact
    24721 ttgcgaggcg ctttccagga ttgaaaactg gccgtcgatt taacggaacg gctatgacag
    24781 gatgcgagca ccacaaagga ggtgaaggtg aaaaacaatt cacagctgat gatgccgcgc
    24841 gaaaaaatgc tgaagtttgg tattagcgcc ttaacggatg tcgagctgct ggcgctattt
    24901 ctgcgtaccg gaacgcgcgg taaagatgta ttaaccctgg caaaagagat gctggagaat
    24961 ttcggctctc tttatggctt gttaacctct gaatatgaac aatttagtgg cgttcatgga
    25021 attggcgtgg cgaaatttgc ccagttaaag gggattgctg aactggcgcg gcgttactac
    25081 aacgtgcgga tgcgtgaaga aagcccttta ctcagcccgg agatgacgag ggaattttta
    25141 caaagccagc tcacgggtga ggagcgggag atctttatgg tgatctttct cgactcccaa
    25201 caccgggtta taacgcatag ccgtcttttt tccggcacgc taaaccatgt tgaagtccat
    25261 cctcgggaaa ttatccgcga agcgataaaa ataaacgcct cggcgctgat ccttgcacat
    25321 aatcaccctt cgggttgtgc tgaacccagt aaagcggata aactcattac tgaacggata
    25381 ataaagagtt gtcagttcat ggatttacgc gtgctcgacc atatcgtgat tgggcgtgga
    25441 gagtatgttt cttttgccga acgcggctgg atttaacccg ctatgcgcga tccttcggga
    25501 tctttgtctg ttcgggactt gagcacatcg ctgagtcagc gtatactacg ccacctttga
    25561 gaatctcggg tttggcattt gggcctggca atcgagagtt cacatagaac tgcgatgacc
    25621 gggctgtaaa gcctgacgag gcgccaatac cccatacgaa gctcgagcta atttgatttt
    25681 tggagaatag acatgtcccg agtctgccaa gttactggca agcgtccggt gaccggtaac
    25741 aaccgttccc acgcactgaa cgcgactaaa cgccgtttcc tgccgaacct gcactctcac
    25801 cgtttctggg ttgagagcga gaagcgtttt gtcaccctgc gcgtatctgc taaaggtatg
    25861 cgtgtaatcg ataaaaaagg catcgataca gttctggctg aactgcgtgc ccgtggcgaa
    25921 aagtactaag tacttagagg aaataaatca tggctaaagg tattcgtgag aaaatcaagc
    25981 tggtttcttc tgctggtact ggtcacttct ataccactac gaagaacaaa cgtactaagc
    26041 cggaaaaact ggaactgaaa aaattcgatc cagttgttcg tcagcacgtg atctacaaag
    26101 aagcgaaaat caaataattc tcgctttgat gtaacaaaaa acctcgctcc ggcggggttt
    26161 tttgttatct gcttgccccc atattgattg catctgttca ttcctggaga tgctatgcct
    26221 gaattacccg aagttgaaac cagccgtcgc ggcatagaac cgcatctcgt tggtgcaacc
    26281 attcttcatg cagtggtgcg caacggacgc ttgcgctggc cggtttcaga agagatctac
    26341 cgtttaagcg accaaccagt gcttagcgtg cagcggcggg ctaaatattt gctgctggag
    26401 ctgcctgagg gctggattat cattcatttg gggatgtctg gcagcctgcg catccttcca
    26461 gaagaacttc cccctgaaaa gcatgaccat gtggatttgg tgatgagcaa cggtaaagtg
    26521 ctgcgctaca ccgatccgcg ccgctttggt gcctggctgt ggaccaaaga gctggaaggg
    26581 cataatgtgc tggcccatct tggaccggag ccgctcagcg acgatttcaa tggtgagtat
    26641 ctgcatcaga agtgcgcgaa gaaaaaaacg gcgattaaac cgtggctgat ggataacaag
    26701 ctggtggtag gggtagggaa tatctatgcc agcgaatcac tgtttgcggc ggggatccat
    26761 ccggatcggc tggcgtcatc actgtcgctg gcagagtgtg aattgttagc tcgggtgatt
    26821 aaagcggtgt tgctgcgttc gattgagcag ggtggtacaa cgctgaaaga ttttctgcaa
    26881 agtgatggta aaccgggcta tttcgcgcag gaattgcagg tttacgggcg aaaaggcgag
    26941 ccgtgtcggg tgtgcggtac gccgattgtg gcgactaaac atgcgcagcg ggcaacgttt
    27001 tattgtcggc agtgccagaa gtaattcatg cgcgccggat ggcataccat ccggcataaa
    27061 cgctacgcta acttcgccat cagcgcctga tggacattct ccggcaggaa atgggtgaca
    27121 tcgccctgat ggcgcgccac ctctttcacc aacgatgaag agataaacga ccactctttc
    27181 gacggcatca gaaacacact ttccagttcc ggcattaagt ggcgattcat atgcgccagc
    27241 tgcatttcat attcaaaatc cgccaccgca cgcaggccac gaatcagcac cgtagcgtgt
    27301 tgattacggg cgaagttcgc catcaaatca ctaaacccga ccacttccac gttccccaga
    27361 tgcgcggttg cctgctgtgc cagcgccaca cgctcttcca gggtaaacat cggtttttta
    27421 ctggggctgg cggcaatcgc cagaataacg tgatcgaaca tttgcgtggc gcgcgtcacg
    27481 atatcgatat gaccattggt aatgggatcg aaagtacccg gataaatcgc ccgtttttgc
    27541 ataacaacct caatgcgttt tcggtggcag gtaaggttcc agcagttgaa gcagacgctg
    27601 tagcgcgccc tggttttgat acagtacttc aacggcatga cggccataga aactacggta
    27661 atcggcgtcg gtgagtaaag aggacacctc ttttgcaagc gtagtggcat cggtaacggt
    27721 aatcagcccg cttgcctgct ccagccgcgc gcaaatgtct ttaaagttaa aagtatgcgg
    27781 ccccatcaat accggaatag cgtgtgcggc agcttccagc ggattatgcc caccacgttc
    27841 aaccagtgaa ccgccaacaa aggcgagatc ggcaatgccg tacagtaaca tcaactcgcc
    27901 catcgtatcg ccaaccacaa cctgcgtgct ggtggagggg acttcccctg aagagcgtgt
    27961 gatatagctt agtccagcct ggcggacaag attaatcgca tccgggaagc gttctggatg
    28021 acggggtacc aggatgagca ataaattcgg gaattgctgt aacaatgcct gatgtgcggc
    28081 aatgaccacg ctctcttcac cttcgtgagt gctggtggca atccataccg ggcggtgtgg
    28141 tgcccactgg cggcgcagcg tcacggcttt agcagccaac tgcggcgtta cagaaatatc
    28201 gaatttcagg ctaccggtaa cggtcacctg attatttttt gcgcccagcg ccacaaaacg
    28261 tgcaccatct tcttcatttt gcgcagcaat cagcgtaata cgacgcagca agcgacggac
    28321 gaatttaccc agtttggcat aacctgcggc cgagcgggca gagagtcgcg cgttagcgat
    28381 caccagcgga attttacgtt tgtgtagcgc cgcaatcagg ttaggccata gttcggtttc
    28441 cataatcaac accagtttgg ggtcgacttt attcaggaaa cggttgagcg catcgggcag
    28501 gtcatacggc agataaacgt gctgaacatc cttcccgaaa gccgattgta cgcgctccga
    28561 accggttggc gtcatggttg taacggtaat cggtaaatca ggataacgat ggcgcagcgc
    28621 gcgcaccaac gggatcgccg ccagagtttc accgacggag acggagtgca gcataatgcc
    28681 gcctggtttt agcggatggc ggtaaaaacc gtaacgttca ccccagcgtt ttcgataggc
    28741 cggagcctta cgtccgcgca cccagagccg tatccagatc agcggctgaa taaggtagag
    28801 aagggcggtg taaagcaatt cgagcatagt aaatagctga cttatggatg tgctggggat
    28861 tctatgtatt tagctgtggc tttaccatta cttttcccgt ttttgactta aatagcaatt
    28921 tacccgccaa ttcttgctga acatctatac tttttttgta tttttatgcg attcattgat
    28981 ttctgctgta cgtgcaggaa ggaatcgcag agcaacagtg aaagaatatg gttgctaatg
    29041 attttatttg attgatttct ttattgaaaa tcaacaatgt gcttaaaaat cattcgggtc
    29101 ggattgccgc gaaagaagtg atacactagc gcgtcaatat cagtgcggta tcagtattca
    29161 ggtagctgtt gagcctgggg cggtagcgtg ctttttctgc ttcaccttaa tcggataatc
    29221 tcaacaaaaa gagttacttg tggataagcc atttcgaaga attttgctca ttaagatgcg
    29281 ttttcatggg gatatgttat taactactcc cgtcattagt tcgctgaaaa aaaattaccc
    29341 tgacgcaaaa atcgatgtgc tgctttatca ggacaccatc ccgatcctgt ctgaaaatcc
    29401 agagattaac gcgctctacg gcataaaaaa taaaaaagca aaagcctcag aaaaaattgc
    29461 caactttttt catctcatca aggtattacg tgccaataag tatgacctta tcgtcaatct
    29521 taccgatcaa tggatggttg ctatactggt tcgcttatta aatgcccgtg tgaaaatttc
    29581 ccaggattat catcatcggc agtctgcttt ttggcgtaaa agtttcaccc atttggtgcc
    29641 gttgcagggt ggaaatgtgg tggaaagtaa cttatccgtg ctgacaccat tgggacttga
    29701 ttcgttggtg aagcagacaa ccatgagtta cccgcctgca agctggaaac gtatgcgtcg
    29761 cgaacttgat cacgctggtg ttggacaaaa ttatgtggtt atccaaccta cggcgcggca
    29821 aatcttcaaa tgctgggaca acgccaagtt ttccgctgtg attgatgcct tacatgctcg
    29881 tggttatgaa gtcgttctga cgtccggccc agataaagac gatctggcct gcgtcaatga
    29941 aattgcgcag ggatgccaga cgccaccagt aacggcgctg gctggaaagg tgaccttccc
    30001 ggaacttggt gcgttaatcg atcatgcgca gctgtttatt ggcgttgatt ccgcaccggc
    30061 gcatattgcc gctgcagtta atacgccgct gatatcgctg tttggtgcga cagaccatat
    30121 tttctggcgt ccctggtcaa ataacatgat tcaattctgg gcgggagatt accgggaaat
    30181 gccaacgcgc gatcagcgtg accgaaatga gatgtatctt tcggttattc cggcggcaga
    30241 tgtcattgct gctgtcgata aattactgcc ctcctccacg acaggtacgt cgttatgatc
    30301 gttgcttttt gtttatataa atattttccc tttggcggtt tgcagcgtga ttttatgcgt
    30361 attgctcaga cagtcgccgc ccgaggtcat catgttcggg tttataccca gtcgtgggaa
    30421 ggcgaatgcc ctgatgtatt tgaactgatc aaagtgccgg ttaaatcgca taccaatcac
    30481 gggcgcaatg cggagtattt tgcctgggtg caaaaacatt tacgcgaaca tcccgtcgat
    30541 aaagtcgttg gattcaacaa aatgccgggg ctggacgttt attatgccgc tgatgtttgt
    30601 tatgccgaga aagtagcgca ggaaaaaggc tttttctatc gcctgacgtc acgttatcgc
    30661 cattatgccg cctttgagcg ggcaaccttc gaacagggta agccgacaca gttgctgatg
    30721 ctgacagata agcaaatcgc cgatttccag aaacattatc agactgaagc ggagcgtttt
    30781 catattctgc caccggggat ttatcctgat cgtaaatata gccagcagcc agccaatagc
    30841 cgtgaaatct tccgtaagaa gaatggaata accgaacaac aatatttatt gttgcaggtc
    30901 ggttcagact tcacgcgtaa aggtgtcgat cgttccattg aagcacttgc ttcgttaccg
    30961 gattcgctgc gccacaacac attgctatat gttgttgggc aggataaacc gcgaaaattt
    31021 gaggcgctgg cagaaaaacg cggcgtgcgc agtaatgttc actttttctc ggggcgcaat
    31081 gatgtctcgg aattaatggc ggcggcggat ttattactgc atcctgccta ccaggaagcg
    31141 gcgggaattg tgctgctgga agcgattact gcaggattac cggtactaac aacagccgtt
    31201 tgtggctatg cgcattatat tgtcgacgct aattgcggcg aggctattgc tgagccattc
    31261 cgccaggaaa cattgaatga gattttacgc aaagcgttaa cgcaatcttc attgcgccag
    31321 gcctgggcgg aaaatgcgcg acattatgct gatacacaag atttatacag tctgccagag
    31381 aaagcggcgg atatcataac gggtggtctg gatggttgaa cttaaagagc cgtttgccac
    31441 gttatggcgc ggcaaagatc cttttgagga agttaaaacc ttgcagggtg aggtatttcg
    31501 tgaactggaa actcgccgta ctctgcgctt tgaaatggcg ggcaaaagct attttctcaa
    31561 atggcatcgc ggcacgaccc tgaaagagat aatcaaaaat ttactctcat tgcggatgcc
    31621 agtattaggc gctgaccgcg aatggaatgc gattcatcga ctgcgggatg tcggcgttga
    31681 tactatgtat ggggtggcat ttggcgaaaa aggcatgaat ccgctgacca gaacttcatt
    31741 tattattacc gaagatctga caccaaccat aagtcttgaa gattacagtg ctgactgggc
    31801 gactaaccct ccagatgttc gcgtaaagcg tatgcttatt aagcgtgtcg cgacgatggt
    31861 gcgcgatatg catgctgcgg gcattaacca ccgtgactgt tatatctgtc atttcctgct
    31921 gcacttgcct ttttccggta aggaagagga gttaaaaatt tcggtaattg acctgcaccg
    31981 ggcgcagctt cgcacgcgcg ttccacgtcg ttggcgggat aaagatctta ttgggcttta
    32041 tttttcttcg atgaatatcg gcctgactca gcgggatatc tggcggttta tgaaagtgta
    32101 ttttgccgcc ccgcttaaag acattctcaa gcaggaacaa ggactgctgt cgcaagcaga
    32161 agcaaaagcc acaaaaatca gggaaagaac gattcgaaaa tcgttgtaac ccgtaactta
    32221 tttttgccaa aattttggat acagaataaa tatgtctcaa ctcaatgata gtgacatcat
    32281 cctttttg
//
LOCUS       DAPHDW010000040        32189 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.040, whole genome shotgun sequence.
ACCESSION   DAPHDW010000040 DAPHDW010000000
VERSION     DAPHDW010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 32189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32189
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.040"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(93..1139)
                     /gene="aroH"
                     /locus_tag="RH057_001855"
     CDS             complement(93..1139)
                     /gene="aroH"
                     /locus_tag="RH057_001855"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HDV3128233.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTAIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLASAVDTRF"
     gene            complement(1296..2129)
                     /gene="ppsR"
                     /locus_tag="RH057_001856"
     CDS             complement(1296..2129)
                     /gene="ppsR"
                     /locus_tag="RH057_001856"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HDV3128234.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            2462..4840
                     /gene="ppsA"
                     /locus_tag="RH057_001857"
     CDS             2462..4840
                     /gene="ppsA"
                     /locus_tag="RH057_001857"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HDV3128235.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPNEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(4897..6543)
                     /gene="fadK"
                     /locus_tag="RH057_001858"
     CDS             complement(4897..6543)
                     /gene="fadK"
                     /locus_tag="RH057_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HDV3128236.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKTGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNTSLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWQDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNVLEKQPADLSALRFFLCGGTTIPKKVARECQQRGIKLLSVY
                     GSTESSPHAVVNLDDPLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEVVAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASGKIQKFLLRKDIMRRLTQDVCEEIE"
     gene            complement(6600..6893)
                     /locus_tag="RH057_001859"
     CDS             complement(6600..6893)
                     /locus_tag="RH057_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV3128237.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILAANPDINEFRKLMKA
                     CPAGLYKQDDAGNIHFDSAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(6890..8179)
                     /locus_tag="RH057_001860"
     CDS             complement(6890..8179)
                     /locus_tag="RH057_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV3128238.1"
                     /translation="MSEDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHTQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQMVNDGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KEREDFSASSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(8235..9173)
                     /locus_tag="RH057_001861"
                     /pseudo
     CDS             complement(8235..9173)
                     /locus_tag="RH057_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310432.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
     gene            complement(9193..9957)
                     /locus_tag="RH057_001862"
     CDS             complement(9193..9957)
                     /locus_tag="RH057_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HDV3128239.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIIIDNDSPEAIAELAEHLKKALN"
     gene            10273..11184
                     /locus_tag="RH057_001863"
     CDS             10273..11184
                     /locus_tag="RH057_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV3128240.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDAFAYTLAIL
                     YYENFKNAYRSEQGYIKKDVLIKDILFYLNNNYREKITLEQLSKKFRASVSYICHEFT
                     KEYRISPINYVIQRRMTEAKWSLTNTELSQAEISWRVGYENVDHFAKLFLRHVGCSPS
                     DYRRQFKNCFAEQEILSEFPQPVSLVG"
     gene            complement(11227..12378)
                     /locus_tag="RH057_001864"
     CDS             complement(11227..12378)
                     /locus_tag="RH057_001864"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HDV3128241.1"
                     /translation="MDFSLTEEQELLLASIRELITTNFPEEYFRTCDQNGTYPREFMR
                     ALADNGISMLGVPEEFGGIPADYVTQMLALMEVSKCGAPAFLITNGQCIHSMRRFGSA
                     EQLRKTAESTLETGDPAYALALTEPGAGSDNNSATTTYTRKNGKVYINGQKTFITGAK
                     EYPYMLVLARDPQPKDPKKAFTLWWVDSSKPGIKINPLHKIGWHMLSTCEVYLDNVEV
                     EESDMVGEEGMGFLNVMYNFEMERLINAARSTGFAECAFEDAARYANQRIAFGKPIGH
                     NQMIQEKLALMAIKIDNMRNMVLKVAWQADQHQSLRTSAALAKLYCARTAMEVIDDAI
                     QIMGGLGYTDEARVSRFWRDVRCERIGGGTDEIMIYVAGRQILKDYQNK"
     gene            complement(12392..13987)
                     /locus_tag="RH057_001865"
     CDS             complement(12392..13987)
                     /locus_tag="RH057_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HDV3128242.1"
                     /translation="MKPVKPPRINGRVPVLSAQEAVNYIPDEATLCVLGAGGGILEAT
                     TLITALADKYKQTQTPRNLSIISPTGLGDRADRGISPLAQEGLVKWALCGHWGQSPRI
                     SELAEQNKIIAYNYPQGVLTQTLRAAAAHQPGIISDIGIGTFVDPRQQGGKLNEVTKE
                     DLIKLVEFDNKEYLYYKAIAPDIAFIRATTCDSEGYATFEDEVMYLDALVIAQAVHNN
                     GGIVMMQVQKMVKKATLHPKSVRIPGYLVDIVVVDPDQSQLYGGAPVNRFISGDFTLD
                     DSTKLSLPLNQRKLVARRALFEMRKGAVGNVGVGIADGIGLVAREEGCADDFILTVET
                     GPIGGITSQGIAFGANVNTRAILDMTSQFDFYHGGGLDVCYLSFAEVDQHGNVGVHKF
                     NGKIMGTGGFIDISATSKKIIFCGTLTAGSLKTEIADGKLHIVQEGRVNKFIRELPEI
                     TFSGKIALERGLDVRYITERAVFTLKEDGLHLIEIAPGVDLQKDILDKMDFTPVISPE
                     LKLMDERLFIDAAMGFVLPEAAH"
     gene            complement(14131..14889)
                     /gene="aroD"
                     /locus_tag="RH057_001866"
     CDS             complement(14131..14889)
                     /gene="aroD"
                     /locus_tag="RH057_001866"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HDV3128243.1"
                     /translation="MKTVTVKDLVIGTGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLTATLEMQEQYADRPIITMSMAKTGVISRLAGEV
                     FGSAATFGAVKKASAPGQISVNDLRTVLTILHQA"
     gene            complement(14920..15786)
                     /gene="ydiB"
                     /locus_tag="RH057_001867"
     CDS             complement(14920..15786)
                     /gene="ydiB"
                     /locus_tag="RH057_001867"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HDV3128244.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGISMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTVTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(15798..17063)
                     /locus_tag="RH057_001868"
     CDS             complement(15798..17063)
                     /locus_tag="RH057_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3128245.1"
                     /translation="MSQNKAFSTPFILAVLCIYFSYFLHGISVITLAQNMSSLAEKFS
                     TDNAGIAYLISGIGLGRLISILFFGVISDKFGRRAVILMAVIMYLLFFFGIPACPNLT
                     LAYGLAVCVGIANSALDTGGYPALMECFPKASGSAVILVKAMVSFGQMFYPMLVSYML
                     LNNIWYGYGLIIPGILFVLITLMLLKSKFPSQLVDASVANELPQMNSKPLVWLEGVSS
                     VLFGVAAFSTFYVIVVWMPKYAMAFAGMSEAEALKTISYYSMGSLVCVFIFAALLKKM
                     VRPIWANVFNSALATITAAIIYLYPSPLVCNAGAFVIGFSAAGGILQLGVSVMSEFFP
                     KSKAKVTSIYMMMGGLANFVIPLITGYLSNIGLQYIIVLDFTFALLALITAIIVFIRY
                     YRVFIIPENDVRFGERKFSTRLNTIKHRG"
     gene            complement(17290..18504)
                     /locus_tag="RH057_001869"
     CDS             complement(17290..18504)
                     /locus_tag="RH057_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3128246.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFGILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMLINALFLYRCTFPPHPGRRLPVIKKTTRSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLSQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSAGSIATFTIPLITAHLSQRSIADIMWFDTAIAAIGFLLALFIGLRSRKKTR
                     HHSLKENVAPGG"
     gene            complement(18604..18960)
                     /locus_tag="RH057_001870"
     CDS             complement(18604..18960)
                     /locus_tag="RH057_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HDV3128247.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DRVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQRVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(19096..19203)
                     /gene="rprA"
                     /locus_tag="RH057_001871"
     ncRNA           complement(19096..19203)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="RH057_001871"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(19389..20501)
                     /gene="ydiK"
                     /locus_tag="RH057_001872"
     CDS             complement(19389..20501)
                     /gene="ydiK"
                     /locus_tag="RH057_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HDV3128248.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            20890..23946
                     /locus_tag="RH057_001873"
     CDS             20890..23946
                     /locus_tag="RH057_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV3128249.1"
                     /translation="MIPQISQAPGVVQLVLNFLQELEQQGFTGDTATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRYVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITPLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDVPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLESLNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESQPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            23943..24353
                     /gene="menI"
                     /locus_tag="RH057_001874"
     CDS             23943..24353
                     /gene="menI"
                     /locus_tag="RH057_001874"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HDV3128250.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGVC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            24453..24641
                     /locus_tag="RH057_001875"
     CDS             24453..24641
                     /locus_tag="RH057_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HDV3128251.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            25189..25557
                     /gene="sufA"
                     /locus_tag="RH057_001876"
     CDS             25189..25557
                     /gene="sufA"
                     /locus_tag="RH057_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HDV3128252.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQAMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFGV"
     gene            25566..27053
                     /gene="sufB"
                     /locus_tag="RH057_001877"
     CDS             25566..27053
                     /gene="sufB"
                     /locus_tag="RH057_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HDV3128253.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLKFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            27063..27809
                     /gene="sufC"
                     /locus_tag="RH057_001878"
     CDS             27063..27809
                     /gene="sufC"
                     /locus_tag="RH057_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HDV3128254.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLAVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            27784..29055
                     /gene="sufD"
                     /locus_tag="RH057_001879"
     CDS             27784..29055
                     /gene="sufD"
                     /locus_tag="RH057_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HDV3128255.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGEISPQQRDALALTLDAVRLVFVDGRYVSALSDATE
                     GSGYEVSINDDRQGVPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            29052..30272
                     /gene="sufS"
                     /locus_tag="RH057_001880"
     CDS             29052..30272
                     /gene="sufS"
                     /locus_tag="RH057_001880"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HDV3128256.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDSEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPNL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNNIAEYEQNLMHYALSQLESVPDLTLYGPRNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVD
                     RLVTGLQRIHRLLG"
     gene            30285..30701
                     /gene="sufE"
                     /locus_tag="RH057_001881"
     CDS             30285..30701
                     /gene="sufE"
                     /locus_tag="RH057_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HDV3128257.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDRSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            30850..31854
                     /gene="ldtE"
                     /locus_tag="RH057_001882"
     CDS             30850..31854
                     /gene="ldtE"
                     /locus_tag="RH057_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HDV3128258.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(31918..32154)
                     /gene="lpp"
                     /locus_tag="RH057_001883"
     CDS             complement(31918..32154)
                     /gene="lpp"
                     /locus_tag="RH057_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HDV3128259.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
BASE COUNT         8170 a         7976 c         8192 g         7851 t
ORIGIN      
        1 tcaatattat ggctgaccag agcaagcagt aatgtgctga gaagacaaca agttgcgcag
       61 aaatgcccat cccatcagga atgggcacgc attcagaagc gggtatctac cgcagaggca
      121 agtttttcga ccaggcgttc ggtatcttcc cagcccagac acgggtcggt aatggattga
      181 ccgtaagtga gcggctgacc gccgacgatt ttttgcgttc cttcgcgcag gaaactttcc
      241 gccataattc cagcaatcgc cgtagagcca ttgcggattt gctgacaaat atcctcacaa
      301 acttctaact ggcgacggtg ctgcttctgg cagttaccgt ggctgaaatc caccaccaga
      361 tgttcaggta aatcaaactc gtgcagcgta tcgcaggctg cggcgatatc atcggcatga
      421 taattcggtt ttttgccgcc acgcataata atgtggccat acgggttgcc gctggtctga
      481 tagatggtca tctgaccatt tttgtctggc gagagaaaca tatggctggc gcgggctgcg
      541 cggatagcat ccacggcaat ccgcgtgttg ccatcggtac catttttaaa acctaccgga
      601 caggagagtg ccgaagccat ttcacggtgg atctgacttt cggtagtacg tgcgccaatc
      661 gcgccccaac tgattaaatc agcaataaac tgaccggtca ccatatcgag gaactcagtc
      721 gcggtaggga cacccagctc atttacctgt aaaagtaatt tgcgcgccag ctccagaccg
      781 tgatttaccc gatagctgcc gtttaaatcc ggatcggaga ttagcccttt ccagccgaca
      841 acagttcgtg gtttttcaaa ataggtgcgc attacgattt ccagccgtga ctggtactgg
      901 ttgcgcagcg actgcagacg ggtggcgtac tccattgcag cggtgagatc gtggatcgag
      961 caggggccaa taatgaccaa cagtcgctta tcttcaccat tcagtatttt ttcaattctg
     1021 cggcgggagt cggtgacatg ggtggcgacg ccaggcgtta cgggataccg tagcgcgagt
     1081 tcggcgggcg ttaccaggct ctcaatacgc gcagtacgga gttcgtcagt tctgttcatt
     1141 acaagtctca gaaatttgtt gcttctacgg caggactacc gggaagtgat gcgcatcacg
     1201 ataaaccaat cgcagctgat ttcaagtgcg aggtgtgtca cacctcgccg ggtggttagc
     1261 atgataacaa aaaaataagc taatgcacta gttctctagt acattcggcg gctaagcccc
     1321 atgatatcga ggatcttggt ggcaatctct tctaccgaat aattggtact gttaatccac
     1381 gggatctgat ttttacggta caacgcttct acttccgcga cttccatcct gcactgacga
     1441 agcgaggcat agcggctgtt ctcccgacgt tcctcgcgaa tcgccgccag acgttccggg
     1501 tcgatagtca ggccgaacaa tttatgctga agcggtttga gcgacgcggg tagcaccaga
     1561 ttgtccatat cgtcggcaat aaaggggtag tttgccgcgc ggataccaaa ctgcattgcc
     1621 agatacagac tggtgggggt tttaccacag cgagaaacac cgagcaggat cacctgagcc
     1681 tggtccagat tgcgcaacga aatgccgtca tcgtgggcga gggtgtaatc aatcgccgca
     1741 atgcgcgcat catatttatt gagattatta gggttaaggc catgggtacg atgagcaatc
     1801 ggcgttggat ccagtttcat ctcttgttgt agcggggcaa ccagcgcctg aacaatatcc
     1861 tggcaaaagc cttcactttg caagatgatg gcgcgaatct ccggcaacac gatggagtag
     1921 aagaccagcg ggcgcacacc tgtctggtga taaattgcgt caatctgatc cttcactgcc
     1981 cgtgcacggc tctcattttc gacaaacggc agcgtaatgc tgctgatagt gacgggaaat
     2041 tgtgacatta ctgcgtgtcc taatacctcc gcagttattg ccgtaccatc agaaatataa
     2101 aaaacgtggc gatcaacagc attatccatt ttgttcttcc cgtgatgcag acataattct
     2161 atgaaagcat aaattaaaaa cgcacagaag cgtaggacgt aatgtctggt ttataaaatg
     2221 aaccttcaat tttatttttt atgaaaacag catttcattt ttatggtttc gtttataccg
     2281 atggtttatg tggaaattgt cgaatagagc aggtttgcgc aacgctggga tcagtcttaa
     2341 aaagtaaaaa aatatatttg cttgaacgat tcaccgtttt tttcatccgg ttaaatatgc
     2401 aaagataaat gcgcagaaat gtgtttctca aaccgttcat ttatcacaaa aggattgttc
     2461 gatgtccaac aatggctcgt caccgctggt gctttggtat aaccaactcg gcatgaatga
     2521 tgtagacagg gttgggggca aaaatgcctc cctgggtgaa atgattacta acctttccgg
     2581 aatgggtgtt tccgttccga atggtttcgc cacaaccgcc gacgcgttta accagtttct
     2641 ggaccaaagc ggcgtaaacc agcgcattta tgaactgctg gataaaacgg atattgacga
     2701 tgttacccag cttgcgaaag cgggcgcgca aatccgccag tggattatcg acactccctt
     2761 ccagcctgag ctggaaaatg ccatccgcga agcttatgca cagctttccg ccgatgacga
     2821 aaacgcctct tttgcggtgc gctcctccgc tactgcagaa gatatgccgg acgcttcctt
     2881 tgccggtcag caggaaacct tcctcaacgt tcagggtttt gacgccgttc tcgtggcagt
     2941 gaaacatgta tttgcttctc tgtttaacga tcgcgccatc tcttatcgtg tgcaccaggg
     3001 ttacgatcac cgtggtgtgg cgctctccgc cggtgttcaa cggatggtgc gctctgacct
     3061 cgcatcttct ggcgtgatgt tctccattga taccgaatcc ggctttgacc aggtggtgtt
     3121 tatcacttcc gcatggggcc ttggtgaaat ggtcgtgcag ggcgcggtta acccggatga
     3181 gttttacgtc cataaaccga cacttgcagc gaatcgcccg gctatcgtgc gtcgcaccat
     3241 ggggtcgaaa aaaatccgca tggtttacgc gccgacccag gagcacggca agcaggttaa
     3301 aatcgaagac gtaccgcagg aacagcgtga catcttctcg ctgaccaacg aagaagtgca
     3361 ggaactggca aaacaggccg tacaaattga gaaacactac ggtcgcccga tggatattga
     3421 gtgggcgaaa gatggccaca ccggcaaact gttcattgtg caggcgcgtc cggaaaccgt
     3481 gcgctcacgc ggtcaggtca tggagcgtta tacgctgcat tcacagggta agattatcgc
     3541 cgaaggccgt gctatcggtc atcgcatcgg cgcgggtccg gtgaaagtca tccatgacat
     3601 cagcgaaatg aaccgcatcg aacctggcga cgtgctggtc actgacatga ccgacccgga
     3661 ctgggaaccg atcatgaaga aagcatctgc catcgtcacc aaccgtggcg gtcgtacctg
     3721 tcacgcggcg atcatcgctc gtgaactggg cattccggcg gtagtgggct gtggtgatgc
     3781 aacagaacgg atgaaagacg gtgagaacgt cactgtttct tgtgccgaag gtgataccgg
     3841 ttacgtctat gcggagttgc tggaatttag cgtgaaaagc tccagcgtag aaacgatgcc
     3901 ggatctgccg ttgaaagtga tgatgaacgt cggtaacccg gaccgtgctt tcgacttcgc
     3961 ctgcctgccg aacgaaggcg tgggccttgc gcgtctggaa tttatcatca accgtatgat
     4021 tggcgtccac ccacgcgcac tgcttgagtt tgacgatcag gaaccgcagt tgcaaaacga
     4081 aatccgcgag atgatgaaag gttttgattc tccgcgtgaa ttttacgttg gtcgtctgac
     4141 tgaagggatc gcgacgctgg gtgccgcgtt ttatccgaag cgcgtcattg tccgtctctc
     4201 tgattttaaa tcgaacgaat atgccaacct ggtcggtggt gagcgttacg agccagatga
     4261 agagaacccg atgctcggct tccgtggcgc gggccgctat gtttccgaca gcttccgcga
     4321 ctgtttcgcg ctggagtgtg aagcagtgaa acgtgtgcgc aacgacatgg ggctgaccaa
     4381 cgttgagatc atgatcccgt tcgtgcgtac cgtagatcag gcgaaagcgg tggttgaaga
     4441 actggcgcgt caggggctga aacgtggcga gaacgggctg aaaatcatca tgatgtgtga
     4501 aatcccgtcc aacgccttgc tggcagagca gttcctcgaa tatttcgacg gcttctcaat
     4561 tggctcaaac gatatgacgc agctggcgct cggtctggac cgcgactccg gcgtggtgtc
     4621 tgaactgttc gatgagcgca acgatgcggt gaaagcactg ctgtcgatgg cgattcgtgc
     4681 cgcgaagaaa cagggcaaat atgtcgggat ttgcggtcag ggtccgtccg accacgaaga
     4741 ttttgccgca tggttgatgg aagaggggat cgatagcctg tctctgaatc cggacaccgt
     4801 ggtgcaaacc tggttaagcc tggctgaact gaagaaataa aataaatacc cggcggcgtt
     4861 ttgtcgccgg gtttatgtga tccccgaaga tgaaacttat tcaatctctt cacagacatc
     4921 ctgcgttaat cgccgcataa tatcttttct taacaaaaac ttttgtattt tacctgaggc
     4981 agtgcgcggt agtttttcga ttaccacgat atgttcagga tatttatatt ttgcgacccg
     5041 tttacggcta aaaaaagcca ccacctcttc cagcgataat gaatgatgcg gcgctttcag
     5101 cacgacataa gcgcatgatc gttcacctaa acgttcatcg ggcattgcaa ccacacaggc
     5161 atcgtgaatt ttaggatgct gcaataaaat atcttccact tcacggctgc taatattttc
     5221 gccgccgcgg acaataatat cttttttgcg tccggtaatt tttatatagc cagcctcatc
     5281 catacggcag agatcgccgc tgtaatacca gccttcttcg tccagcgcac gggcggttaa
     5341 ttcaggttca tcaaaatacc ccataaacac attggggcca cgcgaggctt cttcaccttc
     5401 gcaacctggc ggtaaggtct tgcgtgcgtc atcgaccact ttaatctcta cacctgcggc
     5461 agcgtaacca tcggtgtgca taaagcgcga caaaggatca tcgagattca ccaccgcatg
     5521 cggcgaactt tctgtggaac cataaacact taataattta atgccgcgct gctggcattc
     5581 acgtgccact tttttgggga ttgtggtacc gccgcaaaga aagaaacgca gcgctgaaag
     5641 gtccgcgggt tgtttctcta gtacattcaa aagatcatag acaaacggcg ttgcgcctag
     5701 catacaggtg caacgctgct gctcaagcag cgcgagacac gcatcaggag tgaaaatatc
     5761 taacaacacg ctgcgagcgc caattaagaa tggtgccgtt acgccatgca gaaagcccgt
     5821 tgcgtgacca agtggcgcag gcatcataaa gacatcctgc caggtcagat tcagtcgcgc
     5881 gcaataagcc cgctcgctgg cgagaatatt gttatgcgtt agcatcacgc cctttggcag
     5941 accctcggtt ccggaggtaa acagcaccgc agctaattca tcgccgtggg tcgttatcgc
     6001 cgtggtcagt gaggtattgt cggcgataat ctgacttaat gagagggaag aggtggcggg
     6061 agccagtttg tccacaccga caatttgttg tagttgtgga agctgatttt gcagcggcag
     6121 gattaaatct accggacgcg tttgtttaaa caacgtcggt gcaaagaaca tttttgcctg
     6181 acacttattg agcacccaca ccagttctgc ttcccgccag gaaggcaaca gcggcacgga
     6241 aaccgcaccg gttttcaggc aggcaagata gataacggta aattcacacc agcctggcag
     6301 ttgaaatgcg atgcgatcgc ctgattcaat acccttcgct aacatccagt ttgccagaca
     6361 gctcgcggcg tgatcgagcg cgctataggt gtacgatgct ccatgattat cgaccacggc
     6421 aattttgtct ggcatcgcac gagcggtctg ctgccagtaa tcggccagcg aagcatcgcc
     6481 ccatagccct tgctgacgat acgccgcacg acgttgttcg ttgaacgtta atgtcacttt
     6541 catgttggac tctcgaaaca gaacatcagg cccgagatgc gggcctgtgg gatgcatgat
     6601 tagccgtagc gaaaatcaat accgaaggtg cctgcgggat attgccactg ttcgagaata
     6661 gtgttaccgc acagcacccg acaggtgccg cactccagac aaccggcaga atcaaaatga
     6721 atgttgccgg cgtcatcctg cttataaagt ccggcagggc aggctttcat taatttacgg
     6781 aattcattga tatcgggatt tgccgccaga atgatatgcg ggtggccctc atcaacatgg
     6841 aatttattga cgcctaattt gatgtcgacg ttaaccgtag cgttctggct catagcgcgg
     6901 ttgctccctt aatgccatct ttcagcaagt tgatcagccc aattttcttc gcgtgtccca
     6961 tgatcatttt gcgtaccggc tggtttggtt tgccgtcaat ggtgaacatc tcgttcatga
     7021 tgtcggcgac cattcgtggg tattggctaa acaggcgcgg gttttccatc agcgccggga
     7081 tcttgcggaa atgctgcata tcgcgcatga cgcagctttg ttccagctca cgtttgtatt
     7141 gcgccagact gctggcggag aaatcctcgc gttctttggc ggcgatcacc gttgtggcgg
     7201 cagcctgagc cgatgcaatg gctaaatcca tgccacgaac tgtaaaaccc aaattcaggc
     7261 agaagcctgc ggcgtcacca acgatcatca cgccatcgtt aaccatctgc ggcaccattg
     7321 ccagaccgcc ttctggcacc atatgcgcgg aatattcaag cagtttgccg ccgctaatta
     7381 gtgggcgaat ggcggggtgt tgtttaaaat cttccagcat ttgcggcacg cttttttgcg
     7441 tatgggcgat atcacccaat ccacaaacca gccccaagga tatggaatcc ttgttggtat
     7501 agagaaatcc cccgcccatc aggccgtcag aaggggaacc ggcaaacagc caggcggcac
     7561 cttcctcgcc cgtaatatta aagcgatcgt tgatctgttc tggtgtgagg ccaataacct
     7621 ctttaacacc aacagcgtaa tgatgcggat cggaagcggg aaccattccc agcgagcggc
     7681 caagcatcga gttaacgcca tcagccagaa tcaccacatt cgcttcgaga atatcatccc
     7741 cagcctgcac gccagtgacc ttgtttcctt cacgaaccag cgcatcgacg cgaacgcctg
     7801 ggataaactg cgcgcccgcc tgctcggctt gttccatcaa ccacgggtcc agacgattac
     7861 gcaatacggt ataagatgcg tgttgcggaa catctggttg ctcgcggtga aaatcgaggg
     7921 taacggcgct ttcttcggtt aagaaggaga ttttctcgcg tgtgaccttg cgttctaccg
     7981 gcgctgatac tgcaaagcct ggaatgattg cttcaagtgt gtgggcataa agacgcccgc
     8041 cggtcatgtt tttacatccg gcactgtcgc cgcgttctat caccaggaca tccagcccgg
     8101 ctcgcgccat gaccagtgcg gcaacgctac cagcaacgcc cgcaccgacc acaatggcat
     8161 caaatttgtc ttccgacatg cgttatgctc ccttcccgct gcgtttacag cgggagatgt
     8221 ggttgaaagg ggaattagcg gctcaactgg ctaatcaggg cagggacgac tttgtagata
     8281 tcgcccacca gaccgtagtc ggcataattg aagattggcg catttttatc tttattgatg
     8341 gcgacaatca cttttgcgcc gttgccgcca accatatgct ggatctgccc ggggatcccc
     8401 agcgtcaggt agagatcgga tttcagcaac acgccggaga caccgatata acgttcacgc
     8461 tccatccagt tctcgccttc ggcaattgga cgtgaacagc cgacttcagc attcagcacc
     8521 gccgccagtt cgtggaccat tttcaggtca tcctgcgccg ccagaccacg accgacgcca
     8581 accacacgtt ttgctttgct caggtccacg ctgcttgcgg ctttcgcacg gcgttcgcga
     8641 cagagaattt catgacgcgg agcaacataa ggtaccgttt ctgtcgggca ctgatgagag
     8701 gtatcactgg tgcacggttc ctgaacaccg ggtgcaaggg taatgatcgc cagcgggctg
     8761 ttgatctttt cctgagcgaa cgctaacccg ccatacatcc ggtgttcggc gcaaatgtga
     8821 ccatcgacaa tatccaccgc cgtggcatcg ttcaccagcg ccgcattcag ttgcacactt
     8881 aaccgtgctg ccagagcttt accacgtttc gtcgcggcca acagcaacat agcggggtgt
     8941 ttatctttca gcagggcagc aatgctttcg gcgtaatttt cagtgcgttg cagcgcgtcg
     9001 ttttgctcaa gaacataaat acattttgga ccataaggca taactgcctg cgcctagtcg
     9061 gtattttgta caatggcata cacctgttgg ccccattgct gagcgccgcc aaacagttca
     9121 gcataacgtt caggattatc gctaaagacc cagacgctgt ttaattgact catcgtttct
     9181 tctccgtagg cttcagttca gggctttctt cagatgttcc gccagctcgg caatggcctc
     9241 cggcgaatcg ttatcgataa tgatgtgctt acgttctgtt tgcggcggta cgcgaatgcc
     9301 gaccagttca gcaagtggcg cgctctggct ccagtcaata tcacttgcct gccactgatt
     9361 taccggtttt ttacccgcgc cgagaatggc tttcatcgaa ggaatgcgtg gcacgttaat
     9421 atcggaggtg acgcagagca cggctggaac agagagttca ataacttcaa catcatcttc
     9481 aagcgtgcgt tcaatcacca gtgtattgcc ctgacgctga atagcactca ctgcattaat
     9541 caccggaagt tgcagaattt ctccgaccag caagccaacc tgctgggcat aaaggtcgcc
     9601 ggaaccttca ccaaagatca gtaaatcgaa gccgatcttt tcaattgctg ccgccagcgc
     9661 ttttgcggta tcgagaggca gtgcatgttc aagttgcgca tcctgcacca aatacaggct
     9721 gtgcggcccg cgggatagca cgtctttgcg cactttcgag ttctgcaaca atgagccgcc
     9781 aacggtcagc gcggctatct catcgtcatc tgttgcgagc tggcttgcag cttcaatggc
     9841 attgagatcg aactggctga ttttggcgtc ggcattgtcg aaattcaggg tgtattctgg
     9901 agtgacaaca atgtcctgtt cttcaggcac cagcttaaag caggttatta ttttcatgat
     9961 ttctcctgtg agtcgcatac ggaaccatct ccgatggaag gtaaaaatgg cctgaaaata
    10021 gtgtgcgata gcggtgcggg gttttcgacg gagcaagctg ttttccttgc caacaatctt
    10081 gcaaaaaaaa tgcgatcgct aaaacaaaag tgcaatttgc ggcaatatag ttattccatg
    10141 accgtacaat cttgcattca gtgtaaataa tcttgtttag ctctcgctaa acgcggttgt
    10201 ataaccgcaa tacacgccgt attttatccg gcatatattg tttttgcttg ctcatggaat
    10261 agggaggttg tcatgtatca acgctgtttt gataacgcca gtgaaacgct gtttgtcgcc
    10321 ggtaaaacgc cacggctttc acgttttgca tttagcgatg atccaaaatg ggagtccgga
    10381 catcacgttc atgacaatga aaccgagttg atttacgtca agaaaggggt tgcaaggttt
    10441 accatcgatt cttcgttata tgtcgcacat gcggatgaca ttgtggtgat agaacgcggc
    10501 aggctacatg cggtggcctc tgacgttaac gatccggcaa cgacgtgtac ctgtgcgctg
    10561 tacggctttc agtttcaggg ggctgaggaa aatcagctac tgcaaccgca ttcttgtccg
    10621 gtaattgccg cggggcaggg aaaagaagtc ataaaaacct tatttaatga gctaagtgtg
    10681 attttgccgc aaagtaaaaa tagccaaaca tcttcgttat gggatgcatt tgcctatacg
    10741 ttagccattc tttactacga aaattttaaa aatgcttatc gttcggagca ggggtatatt
    10801 aaaaaagatg ttctgataaa agatattctt ttctatctga ataataatta tcgcgaaaaa
    10861 atcactttag aacagttatc gaaaaaattt cgtgccagcg tcagttatat ttgccatgaa
    10921 tttaccaaag agtatcgtat ttcccctatt aactatgtta ttcaacggcg tatgacggaa
    10981 gcaaaatggt cactcactaa tactgaatta tcacaggcag agatctcctg gcgtgtgggt
    11041 tatgaaaatg tcgatcactt tgccaaactg tttttgcgcc atgtcggttg ttcgcccagc
    11101 gattaccgta gacaatttaa aaactgtttt gcggaacaag aaatcctatc tgaatttcct
    11161 caaccggtaa gtcttgttgg ataaatctgg cggcgcagtt tgttaagaag ctgcgccgcc
    11221 tgcagattat ttgttctgat agtctttcag gatctgccga cctgctacgt aaatcataat
    11281 ttcgtctgta ccgccgccga tacgttcaca acggacatca cgccagaagc gggagacgcg
    11341 cgcttcatcg gtatagccca gaccgcccat gatttgaatc gcatcatcaa tgacttccat
    11401 tgcggtacgt gcgcaataca gttttgccag cgccgcgctg gtgcgcagtg actgatgctg
    11461 atcggcttgc catgccactt tcagcaccat gttgcgcatg ttgtcgatct taatcgccat
    11521 cagcgccagt ttttcctgga tcatctggtt atgaccaatg ggcttaccaa aagcgatacg
    11581 ttggttggca tagcgggcgg catcttcaaa ggcgcattcg gcaaagccgg tgctgcgcgc
    11641 ggcgttgatc aggcgctcca tctcaaagtt gtacatcaca ttgaggaaac ccattccttc
    11701 ttcgcccacc atgtcgctct cttcaacttc cacattgtcg agatagactt cgcaggtgct
    11761 gagcatatgc cagccgattt tatgcagcgg gttaatctta atgccgggct tactggagtc
    11821 gacccaccac agggtgaagg cttttttggg atctttcggt tgcggatcgc gcgccagcac
    11881 cagcatatac gggtactctt tcgcaccggt aataaaggtt ttttgtccgt tgatgtagac
    11941 cttgccgttt ttacgcgtgt aagtggtagt ggcactgttg ttatctgagc cagcacctgg
    12001 ttccgtcaac gccagggcat aggcgggatc acccgtttcc agggtgctct ctgccgtttt
    12061 acgtagctgc tctgcagaac cgaaacggcg catactgtga atacattgac cgttggtaat
    12121 taaaaatgcc ggagcaccgc attttgacac ttccatcagc gccagcattt gggtgacgta
    12181 atccgcaggg ataccaccaa attcttccgg cacgccaagc atggaaatac cgttatccgc
    12241 cagcgcccgc ataaactcac gcggatatgt cccgttttga tcgcaggtgc ggaaatactc
    12301 ttccggaaag ttagtcgtaa tcagttcgcg aatactggcc agcagcagtt cttgttcttc
    12361 agttaaagaa aaatccatca taatactcct gttaatgagc cgcttcaggc aggacaaaac
    12421 ccatcgccgc atcgataaat aatctttcgt ccatcagttt gagttctggc gaaatcactg
    12481 gggtgaagtc cattttgtcg agaatatctt tttgtaaatc gacgccaggg gcgatttcga
    12541 ttaaatgcag gccgtcttct ttcagcgtga atacagcacg ttcggtgatg taacgaacat
    12601 ccagtccccg ttcgagcgcg atttttccgc taaaagtaat ttccggtagt tcccgaataa
    12661 atttatttac ccgtccttcc tggacgatat gcaatttgcc atcggcaatt tctgttttta
    12721 aactgcccgc agttaacgtg ccgcagaaaa taattttctt cgaggtggca ctgatatcaa
    12781 taaatccacc ggtacccatg attttgccat tgaatttatg cacgccgacg ttaccgtgct
    12841 ggtcgacttc agcaaaactc aaataacaaa catccagacc accaccgtga taaaaatcaa
    12901 actgggacgt catatccaga atggcgcggg tattcacgtt cgcgccaaag gcgataccct
    12961 gtgaagtaat tccgccaatc ggacctgttt ctaccgtcag aataaagtca tcagcacaac
    13021 cttcttcccg ggcgaccagg ccaataccgt cagcaatacc gacgccgaca ttccccaccg
    13081 cgcctttacg catttcgaat aatgcacgcc gcgcaactaa tttacgttgg tttaggggca
    13141 ggctaagttt ggtgctgtca tcaagggtga agtcaccaga aataaaacgg ttaaccggcg
    13201 caccgccata cagttggctt tgatccggat cgaccaccac aatatccacc agataacccg
    13261 gaatacggac agatttagga tgcagcgtgg ctttcttaac cattttctgc acctgcatca
    13321 tcacaatacc gccgttattg tgcaccgcct gggcaataac caatgcgtcg agatacatca
    13381 cctcatcttc aaaagtggcg tagccttcac tgtcgcaggt ggtagcgcga atgaaggcga
    13441 tatctggcgc aatcgctttg taatagagat attctttgtt atcaaactcg accagtttaa
    13501 tcaggtcttc tttcgtgact tcattcagtt tgccgccttg ctggcgtgga tcgacaaatg
    13561 tcccgatgcc aatatcacta ataataccag gctggtgggc tgcggcggcg cgtaaggttt
    13621 gtgtaagtac accttgtgga tagttataag caataatttt attttgttct gcgagttcag
    13681 aaatacgcgg cgattgtccc cagtgaccac ataatgccca tttcaccaga ccttcttgcg
    13741 ccagaggact aataccacgg tcggcgcgat cgccaagccc tgtcggacta ataatcgata
    13801 aattacgtgg cgtttgagtc tgtttatatt tatcagcaag agctgtaatt aacgtggtgg
    13861 cttccagaat accgccgcca gcgcctaaca cacaaagtgt tgcttcgtcg ggaatataat
    13921 tcaccgcttc ctgtgccgac aggaccggca ctcgtccatt aatacgaggt ggttttacag
    13981 gtttcattgt taattcctga ttcagatgtg attttcccta cgccagatgg agaattagcg
    14041 cacagagact cacgaaatat ttttattaaa tacggttaaa tatatttttt aagttcggcg
    14101 gggagggtgt tcccgccgaa atattattgc ttatgcctgg tgtaaaatag ttaataccgt
    14161 gcgtaaatca tttaccgaga tttgccctgg tgcagaggct ttttttaccg caccaaaagt
    14221 tgccgccgag ccaaatactt caccagccag acgagaaatt acgccagttt ttgccatcga
    14281 catcgtgatg attggacgat cggcatactg ctcctgcatc tccagggtcg cggtaagcaa
    14341 cgtcagcaca tcgctggtac tttgcggcat cagcgcaatc ttaggaatat cggcgtcgaa
    14401 ggactgcatt ttgcgcagac gggcaatgat ttcttcggct tccggcgttt tatggaagtc
    14461 atggttggac atgacaactt tcacatcatg cgcgtgggcg taggcgacgg tttctttaac
    14521 ctgatcatcg ccggtaaata actccagatc gatcatatca accaggccgc tgtcgatggc
    14581 tgcacgattg agagcgatat aagcctcggt ggaaatcgcc tgctcgccgc cttctttggc
    14641 actgcggaag gtaaacagca gcggtttttc tggcatggtt tcacggagaa tttttgccgc
    14701 cgccatgaca gactccacat tggagaggtc ggcaaagtgg tccacacgcc attccagaat
    14761 atcaaagtcc gcttcacgat aggcgagagc ttcggatttc acgcgggcga tatctttcgc
    14821 catcagcgag acgatgattt taggtgcgcc cgtaccaatg acgagatctt ttacagttac
    14881 ggttttcatt ttttaccctt tctgcacgcg gtcagcctgt caggcaccga accccatgac
    14941 ctgtttaaca tattccagag ggaaatcttt gccagtccat aacgtgaact gttcagcccc
    15001 ttgccacaac aacatgccgt atccatcaat cgttttgcaa ccagcttgtt gcgcctgctg
    15061 caataacttc gtcatatgcg ggttatacac gcattcagtg accagcagtc ccggatgtaa
    15121 cagactgata tcattaacta atgattcatt ctcaaggggt ttcataccca cttttgtgcc
    15181 attggttaaa atgtcggcgg aagccagggc ttcagcaaag gcttgctgat cggcgagatc
    15241 ggtaaccgtg acgacacaat cggtgttttc gttaacccgc tgcgcgaagg cgagggcttt
    15301 atcgaagaac tcatcccgac ggttaaagag tttaatttct tttaaacctt caattgcccc
    15361 ctgcgcgcca atcgccgttg aggcacctcc ggcccctaac agcaccattg ttttgccttt
    15421 gatatcaaaa ccgctctctt taatggcgcg aatatggccc gtgccgtcgg tgttatagcc
    15481 gcgcaggtag ccatcatcat taacgatggt gttgatggca cccaccagtt tggctgctgg
    15541 cgttaattca tcaacatatt cacacgccag ttgtttgttc ggcattgata taccagttcc
    15601 gcgcattttg agggctttta atccttcaat tgctccggga aaactatcgt tatccacttc
    15661 aaaggccata taggtaaatg gcaatcccgc tttttctaag gctttattct gcatttcagg
    15721 cgataaactg tggcggatag gataggccat cagcccaatc aattcgtatt ttgcggtaac
    15781 atccataatt aactccttta acctctatgc ttaattgtgt ttaaccgggt gctaaattta
    15841 cgctcgccaa accgcacatc attctcagga ataataaaaa cgcggtaata gcggataaaa
    15901 acaataattg cggtaatcag ggccagcagc gcgaaagtaa aatcgagaac aatgatatat
    15961 tgaaggccga tgttcgacag ataaccggta atcagcggaa taacaaagtt agccagtcca
    16021 cccatcatca tataaatact ggtgactttg gctttgcttt ttgggaaaaa ctctgacatg
    16081 accgaaacac cgagctgtaa aatgccgcca gctgctgaga aaccgataac aaaggcgccg
    16141 gcattacaca ccagtggaga agggtacagg tagataatgg ctgctgttat tgttgccagt
    16201 gcagagttaa atacattagc ccagatgggc cggaccattt ttttcagtag tgcggcaaaa
    16261 ataaagacac agaccaacga gcccatactg taataagaga tggtttttaa tgcctcagct
    16321 tctgacatac cagcaaaagc catcgcatat ttgggcatcc acaccacaat cacataaaag
    16381 gtcgagaatg cggctacacc gaacagtacc gatgaaacac cttccagcca gactaacggt
    16441 ttgctgttca tttgcggtaa ttcattagct acgctggcgt ccaccaactg gctggggaat
    16501 ttgcttttca acagcatcag cgtgatcagt acaaacagaa tacccggaat aatcagccca
    16561 tagccgtacc agatattatt gagcaacata tagctcacca gcattgggta gaacatttgc
    16621 ccaaatgaca ccatcgcttt aaccagtatg accgccgaac cagaggcttt cggaaagcat
    16681 tccatgagcg cggggtagcc acccgtatcc agcgctgagt tagcgatacc tacgcacact
    16741 gccagaccgt aggcgagagt taaattcggg caagcgggaa taccaaagaa gaatagcaga
    16801 tacattatta ctgccattaa tatcaccgcc cgacgaccaa acttatcgga gatcacaccg
    16861 aagaataaaa tactgatcaa tcgccccaaa ccgataccgg aaattaagta ggcaatgccc
    16921 gcgttgtcag tggaaaactt ttctgccaga gatgacatgt tttgggcaag cgtaataaca
    16981 ctaatgccgt gcaggaaata gctgaagtaa atacaaagaa cagccaggat aaatggcgtg
    17041 ctgaaagcct tattttgaga cattttcaca gctccttgct gaaagataat ttcgttaccg
    17101 tacgtattgt tctccgacgg tttacataat tactatactg aagatgattt tccctgggta
    17161 catggcataa agtttatgtt tccaggaata ttgcatgtca tagtttgttt ttgatcgcga
    17221 ttttccttag ttagcgcaga aagagttatt tatccgtaag atcttgcatg acctgaaaag
    17281 aatattgcat tacccacccg gagcgacatt ttcctttagc gagtgatgcc gcgttttttt
    17341 gcggctgcgt aagccgataa acagtgccag taaaaagccg atggcagcga tggcggtatc
    17401 gaaccacata atatcggcaa tacttctttg ggacagatga gccgtaatca acggaatagt
    17461 aaaggtcgca atactgcccg cactgtaata gatccctgta gctttacctt tagcgtaggg
    17521 gaaacgttca gccattaacg tcaggccaat ttgcacaaca cctccagcag aggtaaaacc
    17581 aatgacaaaa gcaaatatta tcaccacata aaatgtggga tgcaggcaga cggtaaacag
    17641 agcaataaat gagataaagg tgtacagcat cagtaatgtt gttgggcgaa cggtatttcg
    17701 aatgagtgga gcggtaataa atacacaaag cagcgaaccc acggtgtaga tactgagtag
    17761 tttgatcgac atagtgtatg acatgcctgc aacaaattgt ccgtactgtg acagccactg
    17821 gctaaccaga taaaacgttg ccattgagat atagccatat aaggtataac tggctaaatc
    17881 gataattgaa cagcgatgtt ccgtagagcg ggtggttttc tttatgacag gtaagcgacg
    17941 acccggatgg ggtgggaacg tacaacggta taaaaacaga gcgttaataa gcataatgcc
    18001 tgcagcaatc ataaaggacc aaccgaacca cagttcagcc cacactaaca ggctaatgat
    18061 tagcggtaat aaaaattgtc cgctggaaac aaatgcttta attaaaatat tggctgtccc
    18121 aggtgagcgt ggaaaagctt ccatcaaact gggataagtg cctgcatcga gaaaactgtt
    18181 tgccattccc gccagaaagc caaaaacata agcgataatg atgttattgg tctgcaggat
    18241 gccaaaaaag aaggccatat agcaacacat cccgagcatg ataaaagggc ggcgaccaaa
    18301 gcgatcggat aataatcctg caaaaagcaa gacacttaat cgaccaatgc ccagcgatga
    18361 gataactatc gagacacccg cggcattagt ctgccaaagt gtctccagcg aggccatatt
    18421 caggctcatc aaaatgacgc ccataccatg caccaggtaa ttaaaataca acccaagtgc
    18481 ggtagggtaa tagggatttt tcataggttc tacttagcaa aggtgcttta aaaagtgtag
    18541 cgatgttgtg tcagtatcca ttcattattg ttgtaacaac gacaattcct ttctgtggaa
    18601 aaattagcaa agccgctcca gatcgtgtgc atataattca gcggctaccg attgatagat
    18661 cttccagtca ataaaattgc catcagggta gactcgttga aatgcggtta agtcggggaa
    18721 aaaacgcatg gtgttgttgc cgatgcggtt atttattttc tctatgatgg catgaagatg
    18781 tttttttctt tgctgacgca tagccaacag ttgctcgact acacgatcag gaatggcaca
    18841 cttgccattt tcccattgac gccagctttc actgtcaccg gtctgggcaa tccaggtcgc
    18901 gcattcatca atagtcatgg caaaaatatg gcggagtgcc tggagttcat aagcgttcat
    18961 cgtgtaatgg ctgcatgaaa taaacaataa aatataaata acattatcag cctgctgacg
    19021 gcttgaagag agtcacagta tcttgtgcaa cattattgga aagagtgagg ggcgaggtag
    19081 cgaagcggaa aaatgttaaa aaaaagccca tcgtgggaga tgggcaaaga ctacacacag
    19141 caattcgttg tttcactcag gggatttcca tgcttataaa tcaatatgtt gatttataac
    19201 cgtgagctaa tagtaggcat acggactttt tgcgtcgatc agattcgtct cattagttta
    19261 aaaactgtaa agaatttgcg acaggtacta ccagctgaat ggcggtaagc aggaaaatat
    19321 ggggaagagc aagcaaatat caattagcgg tggttaacaa tcaggcggca gagccgcctg
    19381 atgagaaatt acttattcgg tttctcgatt tcgccaagtt cttcgagaat ttcttccggt
    19441 tggtccgtcg gcggcgggac ttcttccacc cacgcggcaa acagacgcca ggaaacggct
    19501 aacagaaccg gaccaataaa cagaccgatc atcccgaaag caatcaaacc accaataacg
    19561 ccagagagga tcaggatcag cggtaaatcg gcacccatgc gaattaacat tgggcggatg
    19621 acgttatcca gcgtgccaac cacaccgctc cacactaaca ataccgttcc ccaggtggta
    19681 tcgccggtcc agtagagcca gataatcgcc ggaatcagta ccggcaacgg gccaagctgg
    19741 acaaggcagg agaggatcat taacaccgtt agcaacgttg cataaggtac gccggatacg
    19801 gcgaggccga taccgccaag cactgcctgt actaacgccg tcaccaccac acccagcgcc
    19861 accgcgcgga tagcctgtgc cgccagcagt acggcggcat ctccgcgaac gcctgccaga
    19921 cgggttgcaa aatggcgaat gccttgtgcc acctgttcac cgcgccagta cagcagggca
    19981 ctgaagagaa gcatcagcgc acaatgcacc ataaagcgcc cgatatgcgc cgcctgccca
    20041 acgaaccagg tggtggtggt gccaatataa gggcggactt tcgccatgat cgccgtcccc
    20101 cccatatcca gcaagttgtg ccagcctgca tacagcttcg cgccaatcac cggaatggta
    20161 ttaagccacg ccagatcggg taacgtcatg tcgccgctgg aaatggcttt aattagcggg
    20221 ccgctgccgt cgacgatact gttaaccagc aaagcaatag ggatgataaa caccatcact
    20281 aataacagcg tcatcaccag aacggcgaga gagcggcggc caaacatgat cttttgcaaa
    20341 cgtaacaata ccggccaggt ggcgataacc accgtaccgg cccatgcaaa gccgagaata
    20401 aagggttgaa caatccacag acatgccaca atcatgatgg ctaaaaacag caccgaaagc
    20461 agaatttgtg cgacatccct gggctgacga acatttacca taactacttt tcaccttttt
    20521 tgtgcgtcaa atcgtcgacg cgacgtgaac acgcgaaact cacctgcata atgatgagca
    20581 atttcagcgg tttttaacag gccgattctg cccttaattc tgatgggcgt gttagaaaaa
    20641 aatgtggtac aacaactcag gcaacacgca aacgatttac tcgtcgtatt tcaagttgca
    20701 tgtgctgcgg ctacgttcgt tcaccccagt cacttactat tgtaagctcc tggagttcac
    20761 tcgcttgccg ccttcctgca actcgaaatc catagagtaa ataaacatca cctttcagtg
    20821 cgctattgca gccgcctgaa agaatagtgt atcgacctac atcacagacc gcaggaaagg
    20881 gtcaatataa tgattccaca gatttcccag gcacccggcg tcgttcaact ggtgcttaat
    20941 tttttgcaag agctggagca acaaggtttt accggcgata cggcgacaag ttatgccgat
    21001 cgtctgacaa tgtcgaccga caacagtatt taccaacttc tccccgatgc ggtggtattt
    21061 ccgcgttcaa ccgcagatgt ggcgctgatc gcccgtcttg ccgcgcagga acgctattca
    21121 tcgctgatct ttaccccccg cggcggcggc accggcacta acggtcaggc gctcaaccag
    21181 gggattattg ttgatatgtc ccgccatatg aaccgcatca tcgaaattaa ccctgaagag
    21241 ggctgggtgc gcgttgaggc cggggtgata aaagatcaac tcaatcagta cctgaaaccg
    21301 ttcggctact tttttgcgcc ggaactttcg accagcaacc gggcaacgct cggtgggatg
    21361 atcaataccg atgcatccgg tcagggatcg ctggtctatg gcaaaacgtc ggatcacgta
    21421 cttggcgtac gcgcggtgtt gttggggggc gatattctcg atacgcaacc tttacccgtc
    21481 gaactggcgg aaacgctggg taaatccaat accacaatcg ggcgaattta taacacggtt
    21541 tatcaacgtt gccgtcagca acgccagtta atcatcgaca acttccccaa acttaaccgc
    21601 tttcttaccg gttacgatct gcgttatgtc tttaacgatg agatgaccga gttcgacctg
    21661 acgcgcattc tgacgggttc agaagggacg ctggccttta ttaccgaagc gcggctggat
    21721 attacgccct tgcctaaagt gcgccgtctg gtgaacgtca aatatgactc ttttgactcc
    21781 gcgctgcgta acgcgccgtt tatggttgag gcgcgggcgc tttcggtaga gacggtggac
    21841 tcaaaagtgc tgaatctggc gcgggaagat attgtctggc attccgtcag cgagttgatt
    21901 accgatgtgc ctgacaaaga gatgctcggg ctgaacattg tggagtttgc tggtgatgat
    21961 gaggcgctga ttgacgagcg agttaatgct ctctgtgtgc ggcttgatga gctgattgcc
    22021 agccaacagg caggtgtgat tggctggcag gtgtgccgcg agctggcggg tgttgaacgt
    22081 atctatgcga tgcgtaaaaa agccgttggt ctgcttggca atgccaaagg tgccgctaag
    22141 ccaattccgt ttgctgagga tacctgcgta ccgccggaac atctggccga ttatattgct
    22201 gaatttcgcg cgctgctcga cagccacggc ttaagctacg gtatgttcgg tcacgtcgac
    22261 gcaggtgtct tgcacgtccg tccagcgctg gatatgtgcg atcctcaaca agagattttg
    22321 atgaagcaaa tctctgatga cgtagtggcg ctgactgcga aatacggtgg tttgttgtgg
    22381 ggcgagcacg gcaaaggttt tcgcgctgaa tacagcccgg cgtttttcgg tgaggaactt
    22441 tttgcagaac tgcgcaaagt gaaagcggca tttgacccgc ataaccgact caacccaggg
    22501 aagatttgcc cgccagaagg tctcgatgcg ccgatgatga aagtggacgc ggtgaagcgc
    22561 ggtacattcg atcggcagat ccccattgcg gtacgccagc agtggcgcgg tgcgatggag
    22621 tgtaacggca acggtttatg cttcaacttt gatgcccgta gtccgatgtg tccgtcgatg
    22681 aagatcaccc agaaccggat tcattcaccg aaagggcgcg caacgctggt gcgtgaatgg
    22741 ctgcgtttgt tggcggatcg cggcgttgat ccactcaaac tggaacaaga actgcctgaa
    22801 tccggcgtca gtttgcggac gttaattgcc cgcacgcgca atagctggca tgcgaataaa
    22861 ggcgaatatg acttctcaca cgaagtcaaa gaggcgatgt cgggctgtct ggcctgtaaa
    22921 gcgtgttcga cccagtgccc catcaaaatt gatgtgccgg agtttcgctc tcgttttctg
    22981 cagctctatc acacccgtta tttacgcccg ctgcgcgacc acctcgtcgc tacggtcgag
    23041 agctacgcgc cgctgatggc acgcgcgccg aaaaccttta acttcttcat taaccagccg
    23101 ctggtgcgca aactctcgga aaaacatatc ggcatggttg atttgccgct gctgtcggtc
    23161 ccctcgctac aacaacaaat ggtggggcat cgctcggcaa acatgacgct ggaacagctt
    23221 gaatctctca atgcagagca gaaagcgcgc acagtgttgg tggtgcagga cccctttacc
    23281 agctattacg atgcgcaagt ggtggcggat tttgtccgtc tggtcgaaaa attaggtttc
    23341 cagcctgtgt tactgccatt ttcaccaaat ggcaaagccc agcatatcaa aggctttctt
    23401 aatcgttttg cgaagacggc gaaaaagacg gcggatttcc tcaaccgtat ggcgaagctg
    23461 ggtatgccaa tggtaggcgt cgatccggcg ctggtacttt gttatcgcga tgaatataaa
    23521 ctggcactgg gcgaggaacg tggcgagttt aacgtcttac tggcgaatga atggctggca
    23581 agcgcacttg agtcacagcc agtggctaca gtcagcggtg aatcatggta tttctttggt
    23641 cactgtaccg aagttaccgc cttgccgggt gcgccagcac aatgggccgc gatatttgcc
    23701 cgttttggcg cgaaactgga aaatgtcagc gtgggttgct gcggcatggc agggacttac
    23761 ggacatgaag cgaaaaacca tgaaaattcg ctcgggatct atgagttatc ctggcatcag
    23821 gctatgcagc gactgccgcg taaccgctgt ctggcgaccg gatattcctg ccgtagccag
    23881 gtaaaacggg ttgaaggcac gggggtacgc catcctgtgc aggctttact ggagattatt
    23941 aaatgatatg gaaacggaaa atcaccctgg aagcactgaa tgctatgggt gaaggaaaca
    24001 tggtggggtt cctggatatt cgctttgaac atattggtga tgacaccctt gaagcgacaa
    24061 tgccagtaga ctcgcggaca aagcagcctt tcgggttgct gcatggagga gcatccgtgg
    24121 tactggccga aagtatcggt tccgttgccg gttatttatg taccgaaggt gagcaaaaag
    24181 tggttggtct ggaaatcaat gctaaccacg tccgctcggc acgagaaggg cgggtgcgcg
    24241 gcgtatgcaa accgttgcat ctcggttcgc gtcaccaggt ctggcagatt gaaatcttcg
    24301 atgagaaagg gcgtttgtgc tgttcgtcac gattgacgac cgccattttg tgacgttgtc
    24361 accagaaaag tgtgacgcat attgcttttg gtcattatct gcacagtaaa cggtggtata
    24421 ctgtgcaggt cttgtctaaa agcgaggatg ctatgtctac tcaacttgat cccacccagc
    24481 tggccattga atttttacgc cgtgaccaga gcaatctttc acctgcccaa tatctcaaac
    24541 gtctgaaaca actagaactt gaatttgctg acctgcttac tctctcttct gctgaattaa
    24601 aagaagaaat ctactttgcc tggcgtctgg gcgtgcattg atcctgactt aatgccgcaa
    24661 gttcgccaaa tcgacctgga ttaacctccg tccaggttcg atgaccttct taatactgct
    24721 tatctaccgc ctgatattgt tcaaaaataa tactaatcgc agtttgtgtt aaaacggcgg
    24781 gttagcttta tgagattatt cttatcgccc cttcaagagc taagccactg agagtgccgg
    24841 agataagcgc cggatggggt agaaaccctt aagcctgtgt tgcacagact taagggtttt
    24901 cttatttctg aacgctgcac ttttttacct atctcttaaa tagtcatttt caatacaact
    24961 ttttcttttt catcacagcc ttaatgctcc ttagataata accattatct aacaatgaga
    25021 tacctaattc ttagcgtgcc tgttaaccca cacatcaggg tctatgctta ttaaaagcag
    25081 gcaaaaagca tgttttacgg taaagcccct gcgtttgctg ggttgtactg ataatcatta
    25141 tcactaacat gctgttatac gctgaaagcg atgaagtgag gtaaatcgat ggacatgcat
    25201 tcaggaacct ttaacccaca agatttcgcc tggcaaggct taacgctgac acccgcagcg
    25261 gcgatacaca tccgtgagct ggtggcaaag cagccgggta tggtcggcgt gcgcttaggc
    25321 gtgaagcaaa cgggctgcgc gggctttggc tatgtgctcg acagtgttag cgagccggac
    25381 aaagacgatc tgctgtttga acacgacggc gcgaagctgt ttgtcccgct gcaagcgatg
    25441 ccgtttattg atggcacgga agtcgatttc gttcgtgaag gacttaatca gatattcaaa
    25501 tttcacaacc ctaaagccca gaatgaatgt ggctgtggcg aaagctttgg ggtataggcg
    25561 gtactatgtc tcgtaatact gaagcaactg acgatgtcaa aacctggacc ggcggcccgc
    25621 tgaattataa agaaggattc ttcacccagt tagccaccga tgagctggca aaggggataa
    25681 acgaagaggt ggtgcgcgca atttcggcga agcgtaatga gccggagtgg atgctgaagt
    25741 ttcgtctaaa cgcctatcgc gcatggctgg agatggaaga accgcactgg ttgaaagcgc
    25801 actacgacaa gctgaattat caggattaca gctactactc agcaccatcg tgcggtaatt
    25861 gtgacgacac ttgcgcgtct gaacctggcg cggtgcagca aactggcgcg aacgcctttt
    25921 taagtaaaga ggtggaggcg gcgtttgagc agttgggcgt tcccgtgcgg gaaggcaaag
    25981 aggtggcggt ggatgccatt ttcgactcag tttcggttgc cactacttat cgcgaaaaac
    26041 tggcggagca gggaattatt ttctgttcct ttggtgaggc gatccacgat cacccggaac
    26101 tggtgcgtaa atatctcggc accgtggtgc cggggaatga caacttcttt gccgcgctta
    26161 atgcggcggt agcctctgac ggtacgttta tttatgtgcc taaaggcgtg cgctgcccga
    26221 tggaactttc cacctatttt cgcattaacg cggaaaaaac cgggcagttt gagcgcacca
    26281 ttctggtggc cgacgaagac agctacgtca gctacattga aggctgttcc gctccggtgc
    26341 gtgacagcta tcagttacac gcggcagtgg tggaagtcat catccataaa aacgccgagg
    26401 tgaaatattc cacggtacaa aactggttcc ctggcgataa caacaccggc ggtattctca
    26461 acttcgtcac caagcgtgct ttgtgcgaag gcgaaaacag caaaatgtca tggacgcaat
    26521 cagaaaccgg gtccgcgatt acgtggaaat atcccagctg cattttgcgc ggcgataact
    26581 ccattggtga gttttactca gtggcgctga ccagcggtca tcagcaagcg gataccggca
    26641 ccaagatgat ccacatcggt aaaaacacca aatcgaccat tatctcgaaa gggatctctg
    26701 ccggacatag tcagaacagt tatcgcggct tagtgaaaat catgccgacg gcaaccaatg
    26761 cgcgcaattt cactcagtgc gactcaatgc tgattggcgc taattgtggg gcgcatacct
    26821 tcccgtatgt cgaatgtcgc aataacagcg cacaactgga gcacgaggca acgacatcac
    26881 gtattggtga agatcaactg ttttactgcc tgcaacgcgg gatcagcgaa gaagacgcca
    26941 tctcgatgat tgttaacggt ttctgcaaag acgtgttctc agaactgccg ctggaatttg
    27001 ccgttgaagc acaaaaactc ctcgccatca gtcttgaaca cagcgtcgga taaggaataa
    27061 acatgttaag tattaaagat ttacacgtca gcgtggaaga taaagctatc ctgcgtggat
    27121 taagcctcgc cgttcgtccc ggcgaagttc acgccattat ggggccaaac ggttcgggca
    27181 aaagtacctt atcggcaacg cttgccgggc gcgaagatta tgaagtgacg ggcggcacgg
    27241 ttgagttcaa aggcaaagat ttgcttgcac tgtcgccgga agatcgtgca ggcgaaggca
    27301 tctttatggc cttccagtat ccggtggaga ttccaggcgt cagtaaccag tttttcctgc
    27361 aaacggcact taatgcagtg cgcagctatc gcggccagga aacgctcgac cgctttgatt
    27421 ttcaggattt gatggaagag aaaatcgctc tcctgaagat gccggaagat ttattaaccc
    27481 gttcggtaaa cgttggtttt tccggcggcg agaaaaagcg caacgatatt ttgcaaatgg
    27541 cggtgctgga accggagtta tgcattcttg atgagtcgga ctccgggctg gatattgacg
    27601 cattaaaagt ggtcgccgat ggcgtgaact cgctgcgtga tggcaagcgc tcattcatca
    27661 ttgttacgca ctaccaacgc attctcgact acatcaagcc tgattacgtc catgtgctat
    27721 atcagggacg aattgtgaaa tccggcgatt tcacgttggt caaacaactg gaggagcagg
    27781 gttatggctg gcttaccgaa cagcagtaac gcgctgcaac agtggcatca cttgtttgaa
    27841 gctgaaggga caaaacgctc accgcaagca cagcagcatt tacaacaatt gctgcgtacc
    27901 ggactgccga cacgtaaaca tgaaaactgg aaatatacgc cgctggaagg gctgaccaat
    27961 agccagtttg tcagcattgc gggagagata tccccacagc agcgagatgc cttagcgtta
    28021 acgttagacg ctgtgcggct ggtatttgtc gatggacgtt acgtgtcagc actgagcgat
    28081 gcgactgaag gcagcgggta tgaagtgagc attaacgacg accgtcaggg ggtacccgac
    28141 gctattcagg cggaagtgtt tctgcatttg acggaaagcc tggcacaaag cgtgacgcat
    28201 atcgccgtga agcgcggtca acggccggca aagccattgc tgttaatgca tattacccag
    28261 ggcgtggcag gtgaagaggt caacactgcc cattaccgac atcatctgga tctggcggaa
    28321 ggtgccgaag caacggtgat cgaacatttt gtcagcctta atgatgctcg tcacttcacc
    28381 ggcgcacggt tcactatcaa tgtcgcagcg aacgcccact tgcagcatat caagctggcg
    28441 tttgaaaacc cggtcagtca ccactttgcc cataacgatt tgttgctggc tgacgatgcc
    28501 accgcattta gccatagttt cctgctgggt ggcgcagtgt tacgacacaa caccagtacg
    28561 caactcaatg gcgaaaacag cacgctgcgg atcaatagcc tggcgatgcc ggtgaaaaac
    28621 gaggtgtgtg atacccgtac ctggctggaa cacaataaag gtttttgtaa cagccgacag
    28681 ttgcacaaaa ctatcgtcag cgacaaaggc cgcgcggtat ttaacggttt gatcaacgtc
    28741 gcgcagcacg ccatcaaaac ggatggtcag atgaccaaca ataatctgct gatgggcaaa
    28801 ctggcagaag tggatacgaa accgcagctg gaaatctatg cagatgatgt gaaatgcagc
    28861 cacggcgcga cggtggggcg tattgatgat gaacagatgt tctatctgcg ctcgcgcggg
    28921 atcaatcagc aggatgccca gcagatgatc atttacgcct ttgctgccga actgacggaa
    28981 gcactgcgtg atgaggggct taaacagcag gtgctggccc gaatcggtca acgactgcca
    29041 ggaggtgcaa gatgactttt tccgtcgaca aagtgcgggc cgactttccg gtgctttcgc
    29101 gtgaggtaaa cggtttgccg ctggcttatc tcgacagcgc cgccagtgcg cagaaaccaa
    29161 gccaggtgat tgactccgaa gccgagtttt atcgtcacgg ctacgcggcg gtgcatcgcg
    29221 gtattcatac cttaagcgcc caggcgaccg agaaaatgga gaacgtgcgc aagcgggcat
    29281 cgctgtttat taatgcccgt tcggcggaag agctggtgtt cgtccgcggc acgacggaag
    29341 ggatcaatct ggtcgccaat agttggggca acagcaacgt gcgggcgggc gataacatca
    29401 tcatcagtca gatggagcac cacgctaaca ttgttccctg gcagatgctt tgcgcacgcg
    29461 ttggcgcaga gctgcgtgtg atcccgctca atcccgacgg tacgctgcaa ctggagacgc
    29521 tacctaatct gttcgatgag aaaactcgcc tgctggcaat tactcatgtc tccaacgtgc
    29581 ttggcacaga aaatcccctg gcggaaatga tcacgcttgc gcatcagcat ggcgcaaaag
    29641 tgctggtgga tggcgctcag gcggtgatgc atcatccggt ggacgttcag gcgctggatt
    29701 gcgattttta cgtattctcc gggcataaac tgtatggccc caccggaatt ggcattcttt
    29761 atgtgaaaga agccttgttg caggagatgc cgccgtggga agggggcggt tctatgatcg
    29821 ccaccgtcag cctgagtgaa ggcactacct ggaccaaagc accatggcgg tttgaagccg
    29881 gtacacccaa taccgggggc atcattggtc ttggcgcggc gctggagtat gtttcggcgc
    29941 tggggcttaa taacatagcc gagtatgaac agaatctgat gcactacgcg ctatcacagc
    30001 tggaatctgt accggatctc actctctatg gcccgcggaa caggcttggc gttattgctt
    30061 ttaatcttgg taaacaccac gcctatgatg ttggcagttt tctcgataac tacggcattg
    30121 ctgtgcgtac cggacatcac tgcgctatgc cattaatggc ctattacaac gtccctgcta
    30181 tgtgtcgggc gtcgctggcc atgtataaca cccatgaaga agtggatcgt ctggtgaccg
    30241 gactgcaacg tattcaccgt ctgctgggat aacagggagg cactatggct ttattgccgg
    30301 ataaagaaaa attgctgcgt aattttttac gctgcgccaa ctgggaagag aaatatctct
    30361 acattattga gctgggccag cgtctgccag aattacgcgc cgaagaccga agcccacaaa
    30421 atagcattca gggctgtcag agtcaggtgt ggattgtcat gcgccagaat gcgcagggaa
    30481 ttattgaatt gcagggcgac agcgatgcgg cgattgtgaa agggcttatt gcggtcgtct
    30541 ttattctcta cgatcagatg acgccgcagg atattgtcaa tttcgatgtg cgtccgtggt
    30601 ttgaaaaaat ggcgctcacc caacatctca ccccatctcg ttcacaaggt ctggaagcga
    30661 tgattcgcgc aattcgcgcc aaagccgctg cacttagcta aactaaaggg acagctttca
    30721 tccggttggc tcacattgcc ggatggtcaa tgtttacact gacctgcaca ggagtgtttt
    30781 ggtttcaggt gaacataagc gccatccgac gtttcagcgt gagtctccgg caaatacagg
    30841 aggtttacaa tgaaacgcgc gtctttgctt acactcacgc ttatcggcgc ttttagcgcc
    30901 atccaggctg cctgggcggt tgattatccg ctaccaccaa ccggaagccg actggttggg
    30961 caaaatcaaa cgtatacggt gcaagaaggg gataaaaacc ttcaggctat tgcccgacgt
    31021 tttgatactg cggcaatgtt gatccttgaa gccaataaca ctatcgcccc ggtgccaaaa
    31081 cctggtacga cgataactat tccttcacaa ctgttattac ctgatgcacc gcgtcagggg
    31141 attatcgtta accttgcaga gctgcgcctt tattattatc cgccgggaga aaatattgtg
    31201 caggtttatc caataggtat tggattgcag gggctggaaa cgccggtgat ggaaacgcgt
    31261 gttgggcaga aaatccctaa cccaacctgg acgcctacgg caggcattcg tcaacgttcg
    31321 ctggagcgtg gcattaaatt accgccagtc gttcctgccg gaccaaataa cccgctagga
    31381 cgttacgcac tgcgcctcgc gcatggtaat ggcgaatacc tcattcatgg taccagtgcg
    31441 ccggacagcg tcggtttgcg cgtcagttca gggtgtattc gcatgaatgc accggatatt
    31501 aaagccttgt tctccagcgt gcggacggga acgccggtga aagtgatcaa cgaaccggtg
    31561 aaatattccg tggaacctaa cgggatgcgt tatgttgaag tacatcgacc actatcagca
    31621 gaagaacagc aaaacgttca gacaatgcca tacacactgc cagcaggctt tacgcaattt
    31681 aaagacaata aggctgtaga tcagaagtta gtcgataaag cgttgtatcg tcgggcaggg
    31741 tatccggttt cggtgagcag tggagcaact cccgcagcca gcaatgcgcc ttcagtagag
    31801 tcagcgcaga atggtgaacc agagcaaggg aatatgttac gcgcgacgca gtagcggtaa
    31861 acggcagaca aaaaaaatgg cgcacaatgt gcgccatttt tcacttcaca ggtactatta
    31921 cttgcggtat ttagtagcca tgttgtccag acgctggtta gcacgagctg cgtcatcttt
    31981 agcagcctga acgtcggaac gcattgcgtt cacgtcgttg ctcagctggt caactttagc
    32041 gttcagagtc tgaacgtcag aagacagctg atcgatttta gcgttgctgg agcaacctgc
    32101 cagcagagta gaacccagga ttaccgcgcc cagtaccagt ttagtagctt tcattattaa
    32161 taccctctag attgagttaa tctccatgt
//
LOCUS       DAPHDW010000041        31959 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.041, whole genome shotgun sequence.
ACCESSION   DAPHDW010000041 DAPHDW010000000
VERSION     DAPHDW010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31959
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.041"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            373..1941
                     /gene="cydA"
                     /locus_tag="RH057_001884"
     CDS             373..1941
                     /gene="cydA"
                     /locus_tag="RH057_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HDV3128260.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQAVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            1957..3096
                     /gene="cydB"
                     /locus_tag="RH057_001885"
     CDS             1957..3096
                     /gene="cydB"
                     /locus_tag="RH057_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HDV3128261.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLP
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            3111..3224
                     /gene="cydX"
                     /locus_tag="RH057_001886"
     CDS             3111..3224
                     /gene="cydX"
                     /locus_tag="RH057_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HDV3128262.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            3224..3517
                     /gene="ybgE"
                     /locus_tag="RH057_001887"
     CDS             3224..3517
                     /gene="ybgE"
                     /locus_tag="RH057_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HDV3128263.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            3667..4071
                     /gene="ybgC"
                     /locus_tag="RH057_001888"
     CDS             3667..4071
                     /gene="ybgC"
                     /locus_tag="RH057_001888"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV3128264.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            4068..4760
                     /gene="tolQ"
                     /locus_tag="RH057_001889"
     CDS             4068..4760
                     /gene="tolQ"
                     /locus_tag="RH057_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HDV3128265.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            4764..5192
                     /gene="tolR"
                     /locus_tag="RH057_001890"
     CDS             4764..5192
                     /gene="tolR"
                     /locus_tag="RH057_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HDV3128266.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            5257..6522
                     /gene="tolA"
                     /locus_tag="RH057_001891"
     CDS             5257..6522
                     /gene="tolA"
                     /locus_tag="RH057_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV3128267.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEEAAAKAAADAKAKAEADAKAAEEAA
                     KKAAADAKKKAEAEAAKAAAEAQKKAEAAAAALKKKAEAAEAAAAEARKKAATEAAEK
                     AKAEAEKKAAAEKAAADKKAAAEKAAADKKAAEKAAAEKAAADKKAAAEKAAADKKAA
                     AAKAAAEKAAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASG
                     ADINNYAGQIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAAL
                     AAAKLAKIPKPPSQAVYEVFKNAPLDFKP"
     gene            6655..7947
                     /gene="tolB"
                     /locus_tag="RH057_001892"
     CDS             6655..7947
                     /gene="tolB"
                     /locus_tag="RH057_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HDV3128268.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            7982..8503
                     /gene="pal"
                     /locus_tag="RH057_001893"
     CDS             7982..8503
                     /gene="pal"
                     /locus_tag="RH057_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HDV3128269.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            8513..9304
                     /gene="cpoB"
                     /locus_tag="RH057_001894"
     CDS             8513..9304
                     /gene="cpoB"
                     /locus_tag="RH057_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HDV3128270.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVGSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGAAASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            9469..9544
                     /locus_tag="RH057_001895"
     tRNA            9469..9544
                     /locus_tag="RH057_001895"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9502..9504,aa:Lys,seq:ttt)
     gene            9680..9755
                     /locus_tag="RH057_001896"
     tRNA            9680..9755
                     /locus_tag="RH057_001896"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9713..9715,aa:Val,seq:tac)
     gene            9758..9833
                     /locus_tag="RH057_001897"
     tRNA            9758..9833
                     /locus_tag="RH057_001897"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9791..9793,aa:Lys,seq:ttt)
     gene            9983..10058
                     /locus_tag="RH057_001898"
     tRNA            9983..10058
                     /locus_tag="RH057_001898"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10016..10018,aa:Val,seq:tac)
     gene            10062..10137
                     /locus_tag="RH057_001899"
     tRNA            10062..10137
                     /locus_tag="RH057_001899"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10095..10097,aa:Lys,seq:ttt)
     gene            10284..10359
                     /locus_tag="RH057_001900"
     tRNA            10284..10359
                     /locus_tag="RH057_001900"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10317..10319,aa:Lys,seq:ttt)
     gene            10393..10468
                     /locus_tag="RH057_001901"
     tRNA            10393..10468
                     /locus_tag="RH057_001901"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10426..10428,aa:Lys,seq:ttt)
     gene            10717..11760
                     /gene="nadA"
                     /locus_tag="RH057_001902"
     CDS             10717..11760
                     /gene="nadA"
                     /locus_tag="RH057_001902"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HDV3128271.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQAECSLDLGCPVEEFNAFCDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKTLPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNGLQSIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            11798..12517
                     /gene="pnuC"
                     /locus_tag="RH057_001903"
     CDS             11798..12517
                     /gene="pnuC"
                     /locus_tag="RH057_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HDV3128272.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(12514..13455)
                     /gene="zitB"
                     /locus_tag="RH057_001904"
     CDS             complement(12514..13455)
                     /gene="zitB"
                     /locus_tag="RH057_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HDV3128273.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHEHDALLDQIQHYL
                     MDHYQIEHATIQMEYQPCHGPDCHLNEGVSGHSHHHH"
     gene            complement(13569..13949)
                     /locus_tag="RH057_001905"
     CDS             complement(13569..13949)
                     /locus_tag="RH057_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HDV3128274.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14265..15317
                     /gene="aroG"
                     /locus_tag="RH057_001906"
     CDS             14265..15317
                     /gene="aroG"
                     /locus_tag="RH057_001906"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HDV3128275.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLESGEPLAYGKSITDACIGWEDT
                     DALLRQLANAVKARRG"
     gene            complement(15475..16227)
                     /gene="gpmA"
                     /locus_tag="RH057_001907"
     CDS             complement(15475..16227)
                     /gene="gpmA"
                     /locus_tag="RH057_001907"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HDV3128276.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(16432..17472)
                     /gene="galM"
                     /locus_tag="RH057_001908"
     CDS             complement(16432..17472)
                     /gene="galM"
                     /locus_tag="RH057_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HDV3128277.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVART
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            complement(17466..18614)
                     /gene="galK"
                     /locus_tag="RH057_001909"
     CDS             complement(17466..18614)
                     /gene="galK"
                     /locus_tag="RH057_001909"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HDV3128278.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPHDDRKVRVMAADYENQLDEFSLDAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            complement(18618..19664)
                     /gene="galT"
                     /locus_tag="RH057_001910"
     CDS             complement(18618..19664)
                     /gene="galT"
                     /locus_tag="RH057_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HDV3128279.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTRTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAEREDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            complement(19674..20690)
                     /gene="galE"
                     /locus_tag="RH057_001911"
     CDS             complement(19674..20690)
                     /gene="galE"
                     /locus_tag="RH057_001911"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308814.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HDV3128280.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVGESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPKIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            complement(20951..22423)
                     /gene="modF"
                     /locus_tag="RH057_001912"
     CDS             complement(20951..22423)
                     /gene="modF"
                     /locus_tag="RH057_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HDV3128281.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPGEDDTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDEIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANEPRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLVTGDHPQGYSNDLTLFGRRRGSGETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQAVSDRQQKLVQQWLDILGIDK
                     RTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            complement(22491..23279)
                     /gene="modE"
                     /locus_tag="RH057_001913"
     CDS             complement(22491..23279)
                     /gene="modE"
                     /locus_tag="RH057_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HDV3128282.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIEHGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            23408..23557
                     /gene="acrZ"
                     /locus_tag="RH057_001914"
     CDS             23408..23557
                     /gene="acrZ"
                     /locus_tag="RH057_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HDV3128283.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            23724..24497
                     /gene="modA"
                     /locus_tag="RH057_001915"
     CDS             23724..24497
                     /gene="modA"
                     /locus_tag="RH057_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3128284.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            24497..25186
                     /gene="modB"
                     /locus_tag="RH057_001916"
     CDS             24497..25186
                     /gene="modB"
                     /locus_tag="RH057_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HDV3128285.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPGETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            25189..26247
                     /gene="modC"
                     /locus_tag="RH057_001917"
     CDS             25189..26247
                     /gene="modC"
                     /locus_tag="RH057_001917"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HDV3128286.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMA
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLDEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELV
                     IKPGLWLYAQIKSVSITA"
     gene            complement(26248..27066)
                     /gene="ybhA"
                     /locus_tag="RH057_001918"
     CDS             complement(26248..27066)
                     /gene="ybhA"
                     /locus_tag="RH057_001918"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HDV3128287.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETAQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIARGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            27221..28216
                     /gene="pgl"
                     /locus_tag="RH057_001919"
     CDS             27221..28216
                     /gene="pgl"
                     /locus_tag="RH057_001919"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HDV3128288.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYSIAPDDGALTFAAESALPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDHSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(28257..29210)
                     /locus_tag="RH057_001920"
     CDS             complement(28257..29210)
                     /locus_tag="RH057_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3128289.1"
                     /translation="MKHELSSMKAFVILAESSSFNNAAKLLNITQPALTRRIKKMEED
                     LHIQLFERTTRKVTLTKAGKRLLPEARELIKKFDETLFNIRDMNAYHRGMVTLACIPT
                     AVFYFLPLAIGKFNELYPNIKVRILEQGTNNCMESVLCNESDFGINMNNVTNSSIDFT
                     PLVNEPFVLACRRDHPLAKKQLVEWQELVGYKMIGVRSSSGNRLLIEQQLADKPWKLD
                     WFYEVRHLSTSLGLVEAGLGISALPGLAMPHAPYSSIIGIPLVEPVIRRTLGIIRRKD
                     AVLSPAAERFFALLINLWTDDKDNLWTNIVERQRHALQEIG"
     gene            29394..30446
                     /locus_tag="RH057_001921"
     CDS             29394..30446
                     /locus_tag="RH057_001921"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HDV3128290.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            30522..31955
                     /locus_tag="RH057_001922"
     CDS             30522..31955
                     /locus_tag="RH057_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HDV3128291.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKRIAYLLIGKIGNTTLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDVVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
BASE COUNT         7673 a         7793 c         8436 g         8057 t
ORIGIN      
        1 catgattaac ataatttgta ggaattgata tttatcaatg tataagtctt ggaaatgggc
       61 atcaaaaaga gataaattgt tctcgatcaa attggctgaa aggcggtaat ttagctataa
      121 attgatcacc gtcgaaaaat gcaaatttgc ttcaacaaaa acctgtttat tgtaaggatt
      181 ttgcggcgta atatatacgt gggatcaatt tgagttttta ttaacatgtt tgcaaccttt
      241 ctttacgccg tttttgtgtg cattcacatg gtatgatgaa agtgttcaaa caaatttcta
      301 ttggggcatg cgtgtgaccc tttctaacgg ggttcactct cggagtcttc atgcgatgag
      361 caaggagtca tgatgttaga tatagtcgaa ctgtcgcgct tacagtttgc cttgaccgcg
      421 atgtaccact tcctttttgt gccactgacg ctcggtatgg cgttcctgct ggccattatg
      481 gaaacggtct acgtcctctc cggcaaacag atttataaag atatgaccaa gttctggggc
      541 aagttgtttg gtatcaactt cgctctgggt gtggctaccg gtctgaccat ggagttccag
      601 ttcgggacta actggtctta ctattcccac tatgtagggg atatcttcgg tgcgccgctg
      661 gcaatcgaag gtctgatggc cttcttcctc gaatccacct ttgtaggtct gttcttcttc
      721 ggttgggatc gtctgggtaa agttcagcat atgtgtgtca cctggctggt ggcgctcggt
      781 tcaaacctgt ccgcactgtg gattctggtt gcgaacggct ggatgcaaaa cccaatcgcg
      841 tccgatttca actttgaaac tatgcgtatg gagatggtga gcttctccga gctggtgctt
      901 aacccggttg ctcaggtgaa attcgttcac actgtagcgt ctggttatgt gactggcgcg
      961 atgttcatcc tcggtatcag cgcatggtat atgctgaaag gtcgtgactt cgccttcgct
     1021 aaacgctcct ttgctatcgc tgccagcttc ggtatggctg ctgttctgtc tgttattgtt
     1081 ctgggtgatg aatccggcta cgaaatgggc gacgtgcaga aaaccaaact ggctgctatt
     1141 gaagccgagt gggaaacgca acctgcgcct gctgccttta ctctgttcgg cattcctgat
     1201 caggaagagg agacgaacaa atttgcgatt cagatccctt acgcactggg catcattgca
     1261 acgcgttccg tggatacccc ggttatcggc ctgaaagagc tgatggtgca gcatgaagaa
     1321 cgcattcgta acgggatgaa ggcgtactct ctgctcgaac aactgcgttc tggttctacc
     1381 gaccaggcgg ttcgtgacca gttcaatagc atgaagaaag acctcggtta cggtctgctg
     1441 ctgaaacgct atacgccaaa cgtggctgat gcgactgaag cgcagattca acaggcaacc
     1501 aaagactcca tcccgcgtgt agcgccgctg tactttgcgt tccgtatcat ggtggcgtgt
     1561 ggcttcctgc tgctggcaat catcgcgctc tctttctgga gtgtcatccg caaccgcatt
     1621 ggcgagaaaa aatggcttct gcgcgccgcg ctgtacggta ttccgctgcc gtggattgct
     1681 gtagaagcgg gctggttcgt ggctgaatat ggccgccaac cgtgggctat cggtgaagtg
     1741 ctgccgacag ctgtggcgaa ctcgtcactg accgcaggcg atctcatctt ctcaatggtg
     1801 ctgatttgcg gcctgtatac cctgttcctg gtggcagaat tgttcttaat gttcaaattt
     1861 gcacgcctcg gcccaagcag cctgaaaacc ggtcgctatc actttgagca gtcttccacg
     1921 actactcagc cggcacgcta agacaggagt cgtcaaatga tcgattatga agtattgcgt
     1981 tttatctggt ggctgctggt tggcgttctg ctgattggtt ttgcagtcac tgacggtttc
     2041 gacatggggg tgggcatgct cacccgtttc ctcggtcgta acgacaccga gcgtcgaatt
     2101 atgattaact ccattgcacc acactgggac ggtaaccagg tttggctgat caccgcgggc
     2161 ggcgcactct ttgctgcctg gccgatggtc tatgccgctg cgttctccgg cttctatgtg
     2221 gcgatgatcc tcgtgctggc gtctttgttc ttccgtccgg tcggttttga ctaccgctcc
     2281 aagattgaag aaacccgctg gcgtaacatg tgggactggg gcatcttcat tggtagcttc
     2341 gttccgccgc tggtaattgg tgtagcgttc ggtaacctgt tgcagggcgt accgttcaac
     2401 gttgatgaat atctgcgtct gtactacacc ggtaacttct tccagttgct taacccgttc
     2461 ggcctgctgg caggcgtggt gagcgtaggg atgatcatta ctcagggcgc aacctatctg
     2521 caaatgcgta ccgtgggcga actgcacctg cgtacccgtg caacggctca ggtggctgcg
     2581 ctggtgacac tggtctgttt cgcactggct ggcgtatggg tgatgtacgg tatcgatggt
     2641 tatgtcgtga aatcgacaat ggaccattac gcagcctcta acccactgaa taaagaagtg
     2701 gttcgtgaag ctggcgcatg gctggttaac ttcaacaaca cgccaattct gtgggctatt
     2761 ccggcgctgg gtgtggttct gccgctgctg accatcctga ctgcacgtat ggataaagcc
     2821 gcgtgggcgt ttgtgttctc ctccctgacg ctggcctgca tcatcctgac agccggtatc
     2881 gcaatgttcc cgtttgtgat gccgtccagc accatgatga acgcaagtct gacaatgtgg
     2941 gatgcaactt ccagccagct gacgcttaac gtcatgacct gggttgcggt ggttctggta
     3001 ccgatcattc tgctctacac cgcctggtgt tactggaaaa tgttcggtcg tatcaccaaa
     3061 gaagatattg aacgtaacac ccactctctg tactaagtaa ggagctaaaa atgtggtatt
     3121 tcgcatggat tctgggaacg cttcttgcct gttcgtttgg ggtaatcacc gcgctggcgc
     3181 ttgaacacgt cgaatcaggc aaagccggtc aagaagacat ctgatgagta agattatcgc
     3241 gactttgtat gcggtaatgg acaagcgccc cctgcgggcg ctttccttcg tgatggcgct
     3301 tctgttagca ggatgtatgt tttgggaccc atctcgtttt gccgcgaaga ccagtgaact
     3361 ggaaatctgg catggtttat tgctgatgtg ggccgtctgt gctggtgtga ttcacggcgt
     3421 gggctttcgt ccgcagaagg ttctttggca agggattttt tgcccattgc ttgccgatat
     3481 tgttctcatt gtcgggctga ttttcttctt cttttaaatc agaattctct ttaaaaaatt
     3541 atgggccgct ccaggcccat aaatttttac gctcccttaa cttgccctca ttcccaaacc
     3601 tcaatcgcgc gcgtatagta gcagcgttta aaagctctaa cttttgttgc attaccggga
     3661 tgtaaagtga atacaacgct gtttcgatgg ccggttcgcg tctactatga agataccgat
     3721 gccggtggtg tggtgtacca cgccagttac gtcgcttttt atgaaagagc acgcacagag
     3781 atgctgcgtc atcatcactt cagtcagcag gcgctgatgg ctgaacgcgt tgcctttgtg
     3841 gtacgtaaaa tgacggtgga atattacgca cctgcgcggc tcgacgatat gctcgaaata
     3901 cagactgaaa taacatcaat gcgtggcacc tctttggttt tcacgcaacg tattgtcaac
     3961 gccgagaata ctttgctgaa tgaagcagag gttctggttg tttgcgttga cccactcaaa
     4021 atgaagcctc gtgcgcttcc caagtctatt gtcgcggagt ttaagcagtg actgacatga
     4081 atatccttga tttgttcctg aaggctagcc ttctggttaa acttatcatg ttgattttga
     4141 ttggtttttc aatcgcatct tgggccatta ttatccagcg gacccgtatt cttaacgcag
     4201 cggcgcgcga agccgaagcg tttgaagata aattctggtc tggaatcgaa ctctctcgcc
     4261 tctatcaaga gagccagggg aaacgggata atctgactgg ttcggaacaa atcttttaca
     4321 gcgggttcaa agagtttgtg cgcctgcatc gtgccaatag ccatgcgccg gaagccgtag
     4381 tggaaggggc gtcgcgtgct atgcgtatct ccatgaaccg tgaacttgaa aatctggaaa
     4441 cgcacattcc gttcctcggt acggttggct ccatcagccc gtatattggt ctgtttggta
     4501 cggtctgggg gatcatgcac gcctttatcg ccctcggggc ggtaaaacaa gcaacactgc
     4561 aaatggttgc gcccggtatc gcagaagcgt tgattgcgac tgcaattggt ctgttcgccg
     4621 caattccggc ggtaatggct tataaccgcc tcaaccagcg cgtaaacaaa ctggaactga
     4681 attacgacaa ctttatggaa gagtttaccg cgattctgca ccgccaggcg tttaccgtta
     4741 gcgagagcaa caaggggtaa gccatggcca gagcgcgtgg acgaggtcgt cgcgatctca
     4801 agtccgaaat caacattgta ccgttgctgg acgtactgct ggtgctgttg ctgatcttta
     4861 tggcgacagc gcccatcatc acccagagcg tggaggtcga tctgccagac gctactgaat
     4921 cacaggcggt gagcagtaac gataatccgc cagtgattgt tgaagtgtct ggtattggtc
     4981 agtacaccgt ggtggttgag aaagatcgcc tggagcgttt accaccagag caggtggtgg
     5041 cggaagtgtc cagccgtttc aaggccaacc cgaaaacggt ctttctgatc ggtggcgcaa
     5101 aagatgtgcc ttacgatgaa ataattaaag cactgaactt gttacatagt gcgggtgtga
     5161 aatcggttgg tttaatgacg cagcctatct aaacatctgc gtttcccttg cttgaaagag
     5221 agcgggtaac aggcgaacag tttttggaaa ccgagagtgt caaaggcaac cgaacaaaac
     5281 gacaagctca agcgggcgat aattatttca gcagtgctgc atgtcatctt atttgcggcg
     5341 ctgatctgga gttcgttcga tgagaatata gaagcttcag ccggaggcgg cggtggttcg
     5401 tccatcgacg ctgtcatggt tgattcaggt gcggtagttg agcagtacaa acgcatgcaa
     5461 agccaggaat caagcgcgaa gcgttctgat gaacagcgca agatgaagga acagcaggct
     5521 gctgaagaac tgcgtgagaa acaagcggct gaacaggaac gcctgaagca acttgagaaa
     5581 gagcggttag cggctcagga gcagaaaaag caggctgaag aagccgcaaa acaggccgag
     5641 ttaaagcaga agcaagctga agaggcggca gcgaaagcgg cggcagatgc taaagcgaag
     5701 gccgaagcag atgctaaagc tgcggaagaa gcagcgaaga aagcggctgc agacgcaaag
     5761 aaaaaagcag aagcagaagc cgccaaagcc gcagccgaag cgcagaaaaa agccgaggca
     5821 gccgctgcgg cactgaagaa gaaagcggaa gcggcagaag cagctgcagc tgaagcaaga
     5881 aagaaagcgg caactgaagc tgctgaaaaa gccaaagcag aagctgagaa gaaagcggct
     5941 gctgaaaagg ctgcagctga taagaaagcg gcagcagaga aagctgcagc cgacaaaaaa
     6001 gcagcagaaa aagcggctgc tgaaaaggca gcagctgata agaaagcagc ggcagaaaaa
     6061 gccgccgcag acaaaaaagc ggcagcggca aaagctgcag ctgaaaaagc cgctgcagca
     6121 aaagcggccg cagaggcaga tgatattttc ggtgagctaa gctctggtaa gaatgcaccg
     6181 aaaacggggg gaggggcgaa agggaacaat gcttcgcctg ccgggagtgg taatactaaa
     6241 aacaatggcg catcaggggc cgatatcaat aactatgccg ggcagattaa atctgctatc
     6301 gaaagtaagt tctatgacgc atcgtcctat gcaggcaaaa cctgtacgct gcgcataaaa
     6361 ctggcacccg atggtatgtt actggatatc aaacctgaag gtggcgatcc cgcactttgt
     6421 caggctgcgt tggcagcagc taagcttgcg aagatcccga aaccaccaag ccaggcagta
     6481 tatgaagtgt tcaaaaacgc gccattggac ttcaaaccgt aatcgcgatg ttgactgttc
     6541 ggacggtcaa catcaggcac cggttgccac ggggttctgg tagttttgtg tattttagtt
     6601 tgttaacatt ctgctaaatt atcgtgggcc atcggtccag ataagggaga tatgatgaag
     6661 caggcattac gagtagcatt tggttttctc atactgtggg catcagttct gcatgctgaa
     6721 gtccgcattg tgatcgacag cggtgtagat tccggtcgtc ctattggtgt tgttcctttc
     6781 cagtgggcgg ggcctggtgc ggcacctgaa gatattggcg gcatcgttgc tgctgacttg
     6841 cgtaacagcg gtaaatttaa tccgttagat cgcgctcgtc tgccacagca gccgggtagt
     6901 gcgcaggaag tacaaccagc tgcatggtcc gcactgggca ttgacgctgt agttgtcggt
     6961 caggtcactc cgaatccgga tggttcttac aatgttgctt atcaacttgt tgacactggc
     7021 ggcgcaccgg gtactgtact tgctcagaac tcgtacaaag tgaacaagca gtggctgcgt
     7081 tatgctggtc ataccgccag tgatgaagtg tttgaaaaac tgaccggcat taaaggtgcg
     7141 ttccgtactc gtattgccta cgttgttcag accaacggcg gtcagttccc gtatgaactg
     7201 cgcgtatctg actatgacgg ttacaaccag tttgtcgttc accgttcacc gcagccgctg
     7261 atgtctccgg cgtggtcacc agacggttct aaactggctt atgtgacctt cgaaagcggt
     7321 cgttccgcgc tggttattca gacgctggca aatggcgctg tacgtcaggt ggcttcattc
     7381 ccgcgtcaca acggtgcacc tgcattctcg ccagacggca gcaaactggc attcgccttg
     7441 tcgaaaaccg gtagtctgaa cctgtacgta atggatttgg cttctggtca gatccgccag
     7501 gtgactgatg gtcgcagtaa caataccgaa ccgacctggt tcccggatag ccagaacctg
     7561 gcatttactt ctgaccaggc cggtcgtccg caggtttata aagtgaatat caacggcggt
     7621 gcgccacaac gtattacctg ggaaggttcg cagaaccagg atgcggatgt cagcagcgac
     7681 ggtaaattta tggtaatggt cagctccaat ggtgggcagc agcacattgc caaacaagat
     7741 ctggcaacgg gaggcgtaca agttctgtcg tccacgttcc tggatgaaac gccaagtctg
     7801 gcacctaacg gcactatggt aatctacagc tcttctcagg ggatgggatc cgtgctgaat
     7861 ttggtttcta cagatgggcg tttcaaagcg cgtcttccgg caactgatgg acaggtcaaa
     7921 ttccctgcct ggtcgccgta tctgtgataa taattaattg aatagtaaag gaatcattga
     7981 aatgcaactg aacaaagtgc tgaaagggct gatgattgct ctgcctgtta tggcaattgc
     8041 ggcatgttct tccaacaaga acgccagcaa tgacggcagc gaaggcatgc tgggtgccgg
     8101 cactggtatg gatgcgaacg gcggcaacgg caacatgtct tccgaagagc aggctcgtct
     8161 gcaaatgcaa cagctgcagc agaacaacat cgtttacttc gatctggaca agtacgatat
     8221 ccgttctgac ttcgctcaaa tgctggatgc acatgcaaac ttcctgcgta gcaacccgtc
     8281 ttacaaagtc accgtagaag gtcacgcgga cgaacgtggt actccggaat acaacatctc
     8341 cctgggtgaa cgtcgtgcga acgccgttaa gatgtacctg cagggtaaag gcgtttctgc
     8401 agaccagatc tccatcgttt cttacggtaa agaaaaacct gcagtactgg gtcatgacga
     8461 agcggcatac tccaaaaacc gtcgtgcggt actggtttac taagagaatt gcatgagcag
     8521 taacttcaga catcaactat tgagtctgtc gttactggtt ggtatagcgg ccccctgggc
     8581 cgcttttgct caggcaccaa tcagtagtgt cggctcaggc tcggtcgaag accgcgtcac
     8641 tcaacttgag cgtatttcta atgctcacag ccagctttta acccaactcc agcaacaact
     8701 ttctgataat caatccgata ttgattccct gcgtggtcag attcaggaaa atcagtatca
     8761 actgaatcag gtcgtggagc ggcaaaagca gatcctgttg cagatcgaca gcctcagcag
     8821 cggtggtgca gcggcgcaat caaccagcag cgatcaaagc ggtgcggcgg catcaacgac
     8881 gccgacagct gatgctggta ctgcgaatgc tggcgcgccg gtgaaaagcg gcgatgcaaa
     8941 cacggattac aatgcagcta ttgcgctggt gcaggataaa tcccgccagg atgacgcaat
     9001 ggtggcattt cagaatttca tcaaaaatta ccctgattca acttacctgc caaacgccaa
     9061 ttattggctg ggtcagttaa actacaacaa gggtaaaaaa gatgatgcgg cgtactattt
     9121 tgcttcggta gtgaaaaact atccgaagtc accaaaggct gctgatgcga tgtttaaagt
     9181 cggcgtcatc atgcaggaca aaggtgacac cgcaaaagcg aaagccgtat accagcaggt
     9241 tatcagtaaa taccctggta ctgatggcgc taaacaggca caaaaacgtc tgaacgcgat
     9301 gtaatgcata acacacgacc agaagtcgca ttatttctgg tcgtgtcgtg cgaatcataa
     9361 gcagttgagt gatctgcatc gaaatttttg ttgcgctcaa gtctgaaatc agtaatatat
     9421 gccgccgttg ccacgggata tcaaacaaac cgaaagcaac gaaaaagtgg gtcgttagct
     9481 cagttggtag agcagttgac ttttaatcaa ttggtcgcag gttcgaatcc tgcacgaccc
     9541 accaatcgct aaggtggaag cggtagtaaa acgtgaagga taacgttgca tcagcaacgg
     9601 cccgaagggc gagacgaagt cgagtcatcc tgcacgaccc accactaaca tagttaattg
     9661 tagtatccag cgtagtattg ggtgattagc tcagctggga gagcacctcc cttacaagga
     9721 gggggtcggc ggttcgatcc cgtcatcacc caccaccggg tcgttagctc agttggtaga
     9781 gcagttgact tttaatcaat tggtcgcagg ttcgaatcct gcacgaccca ccagttttag
     9841 catcgaagac agatgttaag cgtgtaggat aacgttgcgt cagcaacggc ccgtagggcg
     9901 agcgaagcga gtcatcctgc acgacccacc actaattacg gtaggttcgg tggaagtagt
     9961 ttgtagtatc cagcgcagta tcgggtgatt agctcagctg ggagagcacc tcccttacaa
    10021 ggagggggtc ggcggttcga tcccgtcatc acccaccact cgggtcgtta gctcagttgg
    10081 tagagcagtt gacttttaat caattggtcg caggttcgaa tcctgcacga cccaccaatt
    10141 ttaacatcaa actcagatgt taagcgtgaa ggataacgtt gcgccagcaa cggcccgtag
    10201 ggcaagcgaa gcgagtcatc ctgcacgaca caccaatctt aaagattggc cccgagtaga
    10261 aatctttcag gtaacacccg tatgggtcgt tagctcagtt ggtagagcag ttgactttta
    10321 atcaattggt cgcaggttcg aatcctgcac gacccaccat cctgaatgat taaagcagca
    10381 taatcccgca aggggtcgtt agctcagttg gtagagcagt tgacttttaa tcaattggtc
    10441 gcaggttcga atcctgcacg acccaccaat gtaaaaaagc gccctaaagg cgcttttttg
    10501 ctattcaggc atcctcaatt tcactttgta aacctgatga catcgtcaga gcttactgtg
    10561 caagcaactc tacgtcggtg gaattaggcg taattcgagt gacttttccc caccattcga
    10621 ctatcttgtt tagcatataa aacaaattac accgataaca gcgaatatta cgctaatgtc
    10681 ggttttaacg ttaagcctgt aaaacgagat ggtaagatga gcgtaatgtt tgatccagac
    10741 acggcgattt atcctttccc cccgaagccg acgccgttaa gcattgatga aaaagcgtat
    10801 taccgcgaga agataaaacg tctgctaaaa gaacgtaatg cggtgatggt tgcccactac
    10861 tataccgatc ccgaaattca acaactggca gaagaaaccg gtggctgtat ttctgattct
    10921 ctggaaatgg cgcgcttcgg tgcaaagcat cccgcttcta ctttgttagt cgctggggtg
    10981 agatttatgg gagaaaccgc caaaattctc agtccggaaa aaacaattct gatgccgaca
    11041 cttcaggctg aatgttcact ggatctcggc tgccctgttg aagaatttaa cgcattttgc
    11101 gatgcccatc ccgatcgtac tgtcgtcgtc tacgccaaca cttctgctgc ggtaaaagcg
    11161 cgcgcagatt gggtggtaac ttcaagcatt gccgtcgaac ttattgatca tcttgatagt
    11221 ttgggtgaaa aaatcatctg ggcacccgac aaacatctgg ggcgttacgt gcaaaaacag
    11281 acgggtggag acattctatg ctggcagggt gcctgtattg tgcatgatga atttaagact
    11341 caggcgttaa cccgcttgca agaagaatac ccggatgctg ccatactggt gcatccagaa
    11401 tcaccacaag ctattgtcga tatggcggat gcggtcggtt ccaccagtca actgatcgct
    11461 gctgcgaaaa cattgccaca tcagcggctt atcgtggcaa ccgatcgggg cattttctac
    11521 aaaatgcagc aggcagtgcc agataaagag ttactggaag caccaaccgc aggtgagggt
    11581 gcaacctgcc gcagctgcgc gcattgtccg tggatggcca tgaatggcct tcagtccatc
    11641 gcagaggcat tagaacagga aggaagcaat cacgaggttc atgttgatga aaggctgcga
    11701 gagagggcgc tggtgccgct caatcgtatg ctggattttg cggctacact acgtggataa
    11761 cgaataataa ggcgtaacgt tacgctttgg gggaaagatg gattttttta gtgtgcagaa
    11821 tatcctggta catataccaa taggggcagg cggttatgat ctctcatgga tcgaagcggt
    11881 aggcacgatc gccgggttgc tgtgtattgg ccttgccagt ctggagaaga tcagcaacta
    11941 cttctttggc ctgatcaacg tcaccttgtt tggcattatt ttctttcaga ttcagctgta
    12001 tgccagcctg ctattacagg tgtttttctt tgccgcgaat atttacggtt ggtatgcgtg
    12061 gtcgcgacaa accagtcaga acgaggcgga gttgaaaatt cgctggttgc cattgccgaa
    12121 ggcactcagc tggttggcgg tttgcgttgt ttcgattggt ctgatgacgg tatttatcaa
    12181 tccggtgttt gcatttttga cccgcgtggc agtcatgatc atgcaagcat taggattaca
    12241 ggttgtgatg cctgaactgc aaccggacgc tttcccgttc tgggattcat gcatgatggt
    12301 gttatctatc gtggcaatga ttctgatgac gcgtaagtat gtggaaaact ggctgttgtg
    12361 ggtgattatt aacgtgatta gcgtcgttat ttttgcactt cagggcgttt acgccatgtc
    12421 tctggagtac atcatcctga cctttattgc gctcaacggc agccggatgt ggatcaacag
    12481 cgcacgtgaa agaggctcac gcgcgctgtc ccattaatgg tgatgatgtg aatggccaga
    12541 caccccttca ttcaggtggc agtctggccc atgacaaggt tgatattcca tctgaatggt
    12601 ggcgtgctca atctgatagt gatccatcag gtagtgttgg atctgatcca acaaggcatc
    12661 gtgttcgtgt ggcgggatca cctgcacatg caacgtcatc accggcttct cgcctaccat
    12721 ccatacatgt acatggtgca cattgcggac ttccgggatt tcccggcaca tacgacgctt
    12781 cagttcagcg atatccagcg ataccggtgc accttcaagt aattcattca cgctatcttt
    12841 caacagtcgc cacgcgctac gcagaaccag aagcgacacc agtatcgaga gaatggggtc
    12901 agcaggtgtc cagccagtcc agataataat caacgcggca ataatagccc caaccgaacc
    12961 cagcaggtcc ccgagcacat gcagtgccgc tgctcgtacg ttgaggtttt tctcttcgct
    13021 gccgtgatga agtaaccaga aagaaagtat atttgccagc aacccagcca cggcaattgc
    13081 catcatcatg ccgccctcga ccggacgcgg cgtgcggaag cgttctatcg cctcccagac
    13141 aatcaaaatg gtaattacca cgagggctat ggcattcaca aacgccgcga gcgtggtcag
    13201 tcttagccaa ccgaaagtgt ggcgaatggt gggaggacga cgtgaaaatt gcacggcgag
    13261 tagggcgaaa agcagggcgg cagtatcggt caacatatga cccgcatcgg ccagcaatgc
    13321 cagagaacca gaaagaaaac caccaacgac ttctaccagc ataaacccgg cagtcacgcc
    13381 gaaagcatac aacaagcgac gagcattatt atcttctggc aggtgtgagg acgtgtgtga
    13441 gtgtgagtgc gccatgacga gtcatccctt aattattctc acttaacttt atgacatcat
    13501 actgctttta gaagtgaaaa attaaaaggg agagactccg ctctcccatt attggctatt
    13561 ttgcagggtt actgcgtggt accgtcggtt ttggtatcga catcattatt gatgccatca
    13621 ccggtttgta cctttttatt gatatccgga cagcgaccat ctttgcacat ggtgttcttg
    13681 tgctcttcat ctttggtcat tccgtcattg ttcattgaag aaccatccga atgcagcatt
    13741 gtgccaccag aaccggtatt taccccgtta ttgtcgacgt tatttggcgc gacattttca
    13801 cgggcgtcag gggctacctg gcccgcatca gctgcggcgt ttgcctggcc gttattagtt
    13861 tgcgctccgc tatcggcagc cagtgcggca ccgctggcaa ggcttagagt ggcagtcaga
    13921 aataatgtgg ccagttttgt cattttcata ggatgctcct gttatggtcg ttatgtcgga
    13981 taacctcttc caacagtgca tttgcaggtg aatataaggc attggtttaa gatttcagcc
    14041 aggttatgaa acgcagcaga gaatcttgaa ataattaaca aacaaaggag ttacagttag
    14101 aaattgtagg agagatctcg tttttcgcga caatctggcg tttttcttgc taattccagg
    14161 attaatccgt tcatagtgta aaaccccgtt tacacattct gacggaagat atagattgga
    14221 agtattgcat tcactaagat aagtatggca acactggaac agacatgaat tatcagaacg
    14281 acgatttacg catcaaagaa atcaaagagt tacttcctcc tgtcgcattg ctggaaaaat
    14341 tccccgctac tgaaaatgcc gcgaatacgg ttgcccatgc ccgaaaagcg atccataaga
    14401 tcctgaaagg taatgatgat cgcctgttgg ttgtgattgg cccatgctca attcatgatc
    14461 ctgtcgcggc aaaagagtat gccactcgct tgctggcgct gcgtgaagag ctgaaagatg
    14521 agctggaaat cgtaatgcgc gtctattttg aaaagccgcg taccacggtg ggctggaaag
    14581 ggctgattaa cgatccgcat atggataata gcttccagat caacgacggt ctgcgtatag
    14641 cccgtaaatt gctgcttgat attaacgaca gcggtctgcc agcggcaggt gagtttctcg
    14701 atatgatcac cccacaatat ctcgctgacc tgatgagctg gggcgcaatt ggcgcacgta
    14761 ccaccgaatc gcaggtgcac cgcgaactgg catcagggct ttcttgtccg gtcggcttca
    14821 aaaatggcac cgacggtacg attaaagtgg ctatcgatgc cattaatgcc gccggtgcgc
    14881 cgcactgctt cctgtccgta acgaaatggg ggcattcggc gattgtgaat accagcggta
    14941 acggcgattg ccatatcatt ctgcgcggcg gtaaagagcc taactacagc gcgaagcacg
    15001 ttgctgaagt gaaagaaggg ctgaacaaag caggcctgcc agcacaggtg atgatcgatt
    15061 tcagccatgc taactcgtcc aaacaattca aaaagcagat ggatgtttgt gctgacgttt
    15121 gccagcagat tgccggtggc gaaaaggcca ttattggcgt gatggtggaa agccatctgg
    15181 tggaaggcaa tcagagcctc gagagcgggg agccgctggc ctacggtaag agcatcaccg
    15241 atgcctgcat cggctgggaa gataccgatg ctctgttacg tcaactggcg aatgcagtaa
    15301 aagcgcgtcg cgggtaaggt ttaattgtcg gatgcgccgt cagagtggcg tatccgatga
    15361 atcaccacag gcctgataag tcgcgcagcg tcgcatcagg caatgtgctc cattgttagc
    15421 aacaaaaatg ccgactcact tgcagtcggc tttctcattt taaacgaatg acgtttactt
    15481 cgctttaccc tggtttgcaa ccgccgctgc tttcgctgcg atctcgtcag cattacccag
    15541 ataatagcgt ttcagcggtt tgaaattctc gtcgaactca tacaccagcg gcacgccagt
    15601 cgggatatta agctcaagaa tctcttcttc gctcatgtta tcaagatatt tcaccagcgc
    15661 acgtaaagag ttaccgtgtg cagcgatgat cacgcgctca ccgctcttca tacgcggcag
    15721 aatagtttca ttccagtaag ggatcacgcg gtcaatggtc agcgccaggc tttccgtcag
    15781 cggcagttct ttttcgctca gtttcgcgta acgcggatcg tgacccggat aacgctcatc
    15841 atctttagtc agttccggcg gagtcactgc aaaaccacga cgccactgtt tcacctgctc
    15901 gtcgccatac ttttcagcag tttccgcttt gttcagaccc tgcaacgcac cgtagtgacg
    15961 ttcgttcagt ttccaggatt tctcaacggg cagccatgcc tgatccagtt cgtccagcac
    16021 attccacagg gtatggatag cgcgtttcag cacagaggtg taagcaaagt caaagctgta
    16081 accttcctct ttcagcagct tacctgctgc ttttgcttcg cttacgcctt tctcagacag
    16141 atccacgtcg taccaaccgg tgaaacggtt ttctttgttc cactgacttt cgccatgacg
    16201 aaccagaacc agcttagtta cagccatata cttactcctc aaatcatttt ttaatgataa
    16261 taattctcat tatattgccg tgacgaagca acagcaatgc ttacgcataa ccatagcgaa
    16321 aatagtgtcg cagtgtaagg ttgttgtgaa tattgagttg caaatatgtc ggtgtttgct
    16381 ggtgatttga gcaatataag gataaagccc tcattacgag ggcttcaggt gctactcagc
    16441 aataaactga tattccgtca ggctggaata ctcttcgcca ggacgcagga agcagtccgg
    16501 ttgcggccat tcagggtggt ttgggctgtc cggcagaaac tcgctttcca gtgccaggcc
    16561 ttgccagtcg gcgtaaggtt cagttcctct tgatggcgtg ccgccgagga agttgccgga
    16621 gtagaattgc agagccggag cggtggtgta gaccttcagc tgcaattttt catctgctga
    16681 ccagacatgc gccgccactt tcttgccatc gcctttggcc tgtaacaaga atgcgtgatc
    16741 gtaacctttc actttgcgct gatcgtcgtc ggcaagaaac tcactggcga tgattttggc
    16801 gctacggaaa tcaaaagacg ttctggcgac agatttcagg ccgtcgtgcg gaatgccgcc
    16861 ttcatcaacc ggcagatatt cgtccgccag aatctgcaac ttgtgattgc gcacgtcaga
    16921 ctgctcgccg tcaagattga aatagacgtg attagtcata ttcaccgggc aaggtttatc
    16981 aactgtggcg cgataagtaa tagagatacg gttatcgtcg gtcagacgat attgcaccgt
    17041 cgcgccgaga ttacccggga agccctgatc gccatcatct gaactcaggg caaacagcac
    17101 ctgacgatcg ttctggttca caatctgcca gcgacgtttg tcgaaccctt ccgggccgcc
    17161 gtgcagctgg ttaacgccct gacttggcga aagcgtcacg gtttcaccgt caaaggtata
    17221 acggctattg gcgatgcggt tggcataacg accaatagag gcccccagaa acgcagcctg
    17281 atcctgatag cattccgggc tggcacagcc gagcagcgcc tcgcggacgc tgccatcgga
    17341 aagcggaata cgagctgaaa gtaaagtcgc accccagtcc atcagcgtga ctaccatccc
    17401 tgcgttgtta cgcaaagtta acagtcggta cggctgacca tcgggtgcca gtgcgggagt
    17461 ttcgttcagc actgtcctgc tccttgtgat ggtttacaaa cgtaaaaagt ctctttaata
    17521 cctgtttttg cttcatattg ttcagcgaca gcttgctgta cggcaggcac cagctcttcc
    17581 gggatcagcg cgacgataca gccgccaaat ccgccgccgg tcatgcgtac gccacctttg
    17641 tcgccaatca cagctttgac gatttctacc agagtgtcaa tttgcggcac ggtgatttcg
    17701 aaatcatcgc gcatagaggc atgagactcc gccattaact cgcccatacg tttcaggtcg
    17761 ccttgctcca gcgcgctggc agcttcaacg gtgcgggcgt tttcagtcag gatatgccgc
    17821 acgcgtttcg ccacgattgg gtccagctca tgtgcaacag cgttgaactc ttcaatggtg
    17881 acatcgcgca gagctggctg ctggaagaaa cgcgcaccgg tttcgcactg ttcacgacgg
    17941 gtgttgtatt cgctgccaac cagggtacgt ttgaagttac tgttgatgat gacgacagcc
    18001 acacctttcg gcatggaaac tgctttagtc cccagtgagc ggcagtcaat cagcaaggca
    18061 tgatctttct tgccgagtgc ggaaattagc tgatccatga tcccgcagtt acagccaaca
    18121 aactggtttt ctgcttcctg accgttaagc gcgatttgtg cgccgtccag cggcagatga
    18181 taaagctgct gcaatacggt tccgaccgcg acttccagtg aagcggaaga acttaacccg
    18241 gcaccctgcg gcacattgcc gctgatcacc atgtccacgc cgccgaagct gttgttacgc
    18301 agttgcagat gtttcaccac gccacgaacg tagttcgccc attgatagtt ttcatgcgcg
    18361 acaatgggcg catcgaggga aaactcgtcg agctgatttt cataatcggc tgccattacg
    18421 cgaactttac ggtcatcgtg tggcgcacag ctgatcacgg tttgataatc aatcgcgcag
    18481 ggcagaacga aaccgtcgtt gtagtcggtg tgttcaccaa tcaaattcac gcggccaggc
    18541 gcctgaatgg tgtgagtggc agggtagcca aatgcgttgg caaacagaga ttgtgttttt
    18601 tctttcagac tcatttctta cactccggat tcgcgaaaat ggatatcgct gactgcgcgc
    18661 aaacgctctg ctgcctgttc tgcggtcagg tctcgctggg tctctgccag catttcataa
    18721 ccaaccataa atttacgtac ggtggcggag cgcagcagag gcggataaaa gtgcgcgtgc
    18781 agctgccagt gttgattctc ttcgccatta aatggtgcgc cgtgccagcc catagagtag
    18841 gggaaggagc actggaagag gttgtcataa cgactggtca gctttttcaa cgccagcgcc
    18901 aaatcgctgc gctgggcgtc ggtcaaatcg gtgatccgca aaacgtgggc tttgggcagc
    18961 agtagcgttt cgaacggcca ggcagcccag taaggcacaa cggctaacca gtgttcggtt
    19021 tcgacaacgg tacggctacc gtctgccagc tcgcgctgaa cataatccac cagcattggt
    19081 gatttctgtt cggcaaaata ttctttttgc aggcggtctt cgcgctcagc ttcgttaggc
    19141 aggaagctat ttgcccaaat ctgtccgtgc ggatgcgggt tagagcagcc catcgccgca
    19201 cctttgtttt caaagacctg cacccacggg tacgtttttc ccagctctgc ggtttgctcc
    19261 tgccaggttt tgacgatttc cgtcaatgcc gcaacgctca gttctggcaa cgttttactg
    19321 tgatccggtg aaaagcagat cacccggctg gtaccgcgcg cgctctggca acgcatcaaa
    19381 ggatcgttgc tttctggcgc atctggcgtg tcagacatca aggccgcaaa gtcattagtg
    19441 aaaacgtaag ttctggtgta atcggggttt ttatcgcctg tcacccgcac attacctgcg
    19501 cagaggaagc aatctggatc gtgcgcaggt aacacctgtt tggctggcgt ttcctgcgcc
    19561 ccctgccagg ggcgcttagc gcggtgcggt gaaaccagaa tccattgccc ggtgagcggg
    19621 ttgtagcggc gatgtggatg atcaacggga ttaaattgcg tcatggtcgt tccttaatcg
    19681 ggatatccct gtggatggcg tgactgccag tgccaggtgt cctgcgccat ttcatcgagt
    19741 gtgcgcgtta cgcgccagtt cagttcacgg tcggctttgc tggcgtccgc ccagtaggcc
    19801 ggaaggtcgc cttcgcgacg cggtgcaaaa tgatagttaa ccggtttgcc gcaggctttg
    19861 ctgaaggcat taaccacgtc cagcacgctg ctgcctacgc cagcaccgag gttgtagatg
    19921 tgtacgcctg gcttgttcgc cagtttttcc atcgccacga cgtgaccgtc cgccagatcc
    19981 attacgtgga tgtaatcgcg tacgccagta ccatcttcgg tcggataatc gttaccaaaa
    20041 atcgccagcg agtcgcgacg gcctacagca acctgggcga tgtatggcat caggttattc
    20101 ggaatgcctt gcggatcttc gcccatatcg cccgacggat gcgcgccaac cgggttgaag
    20161 tagcgcagca gggcaatgct ccagtccggc tgggcttttt gcaggtcggt gaggatctgt
    20221 tccaccatca atttgctttt gccataaggg ctttgcggtg tgccggtcgg gaagctttca
    20281 acgtatggaa ttttgggctg atcgccataa acggtagcgg aggagctaaa aataaagttt
    20341 ttgacgttag cggcgcgcat ggcgctaatc aggcgcagag tgccgttgac attgttgtcg
    20401 taatattcca gcggtttttg taccgattcg ccgacggctt tcagcccggc gaagtggatc
    20461 acggtgtcga tagcgtgatc gtgcagtatc tcggtcatca acgcttcgtt acgaatatcg
    20521 ccttcaacaa acgttggatg tttgccgcct aaacgctcga taacaggcag tacgctgcgc
    20581 ttactgttac agaggttatc aagaatgatg acatcatgac cgttttgcag taattgcacg
    20641 caggtatgac ttccaatgta accgctacca ccggtaacca gaactctcat aattcgctcc
    20701 attaggctta tggtatgaaa taaccatagc ataacaaaga tgcgaaaagt gtgacatgga
    20761 ataaattagt ggaatcgttt acacaagaat ttagccgttt tttatgcgcg attaagtgat
    20821 tataaaacag agggtttatg aatgattgcg ctttttatct gaaaaaagaa gcggtttcat
    20881 gcctgcatgc gtcgaaccgt tggccggaga gggcgcttag gccgcctccg gcaaggtcag
    20941 cactaccgac tcaatatatt tttgtcagca catagcgata gagtccaccg tccggcacga
    21001 actcaaggcg atgggtaata caggcaggcg catcttcagc gtggtgcgaa acaaacaaca
    21061 attgcgtttc accttcgcta atcagcacat caacaaaacg gcggataagc tggcgattga
    21121 gcggatcaag cccctgtagt ggttcatcga gaataagcaa cgtcggatgt ttcaccagtg
    21181 cgcggacgat cagcgccaga cgctgctgtc cccaggaaag actatggaac ggagcgtcag
    21241 ccgtacgttt atcaatgccg agaatatcca gccactgctg caccagtttt tgctggcgat
    21301 ccgaaacggc ctgataaatg ccaatcgaat caaaatagcc agaaagaatc acattacgca
    21361 cggtagtgct gacccggtaa tccagatgca aactactgct gacgtaaccg atatgctttt
    21421 tgatatccca gatggtttcg ccgctgccgc gacgtcgtcc gaaaagcgtc aaatcgttgc
    21481 tgtaaccttg cggatgatcg ccagtaacca ggcttaataa cgtcgatttt cctgcaccat
    21541 ttggcccgac aatttgccag tgttcgcctg gattcacctg ccagctaagg ttattaagaa
    21601 tggggcgatc gttataagaa accacgccat tgttcagcac aatgcgcggt tcgttggcgg
    21661 gtaaggcgtg acgtgctgaa ggttcatccg gctccggcag ttgcacacct tcaagctgtt
    21721 cactatgcgc cagttgcgcg acgagtgctt gttggagcag ttcctcttta gcgccagttt
    21781 ccgctaacgt gcaatccgcc agcacgccag caaactggac aaactccggg atctcatcga
    21841 agcgattgag caccagtacc agagtaatac cggactgatg taacgaggcg agtcgctcag
    21901 ccagctgctg acgtgaggca acatccaggc catcgaacgg ctcatcaaga atcaacaagt
    21961 caggctccga catcagcgcc tgacacagca gggtttttcg cgtctcgcca gtggaaaggt
    22021 atttaaagcg tcggtcgagg agggcggtaa taccgaactg ctgcgccagt tgcatgcaac
    22081 gcggtgcatc ctttacttca tcctgaatga tctcagccgt agtgcgtccg gtgtcatctt
    22141 cgccagggcc gagcatatcg gtgttattcc gctgccattc gtcgctgacg agtttttgca
    22201 attgctcgaa ggagagacga gtgatgtggg aaaactggct ttgccgttca cctttcaaaa
    22261 gcggaagttc ccccgccagc gcgcgggcca gggccgattt cccgcttcca ttcgaaccga
    22321 caaacgccca actatcaccc gcgtttaacg ttagctgagg caattgcagc gtttttgtgt
    22381 cgctaagacg aaacgtgcct tgcaaaattt gcaacgatga cattttatat cccattttgt
    22441 gcagcgatta ctgacaggga tacgtgtttc ataacaaatt gtcaacacgc ttagcacagc
    22501 gtggcgataa tcacgctgtc ggcattaaag taggccgtga cattctgccc ttgctgaaga
    22561 gaagtcgctt catttaccgg cactgtggcg cacagtgttt gcccgtcggg tagcgccatt
    22621 aatacttcgc actgctctgc gccgtgctca atatgactaa taatacccgg taattggttg
    22681 tcagcgtttt gcgcgaccgc ctcgtcctga gtaataccta cccacggcgc ttttagcaat
    22741 atcaacactt ctttgccttc atccagcccc agacgcgcgc cgctttgtgc ggtaattgcg
    22801 actttcaggc gtgtttttcc gtcagccagt aagacatcaa catgctgttg aacgtcatca
    22861 tgatcgcggg cggtgatggt gccgaaccac tggttacggg cgctggtttg cagtgaaaaa
    22921 cgtgagatcg cggccagcag gctgttcagc ggcagggcgt catcgtcact taacacatca
    22981 aaggcttttt gctggatttg cgccagtaag tcatagagct gaatcagtcg ctgaccatag
    23041 cgggtcagta ctgcgccgcc gccaccttta ccgcctgttg cgcgctcgac cagaatatgc
    23101 tcacttaact gattcatctc gttaatggca tcccaggcgc ttttatagct gataccggca
    23161 tctttcgctc cctggctaat ggaaccggaa agcgcaatgt gttttagtag cgaaatccgg
    23221 cgcgggtcgg cgaataattt ttgttggagc ttaagggtga gaaggatttc ggcctgcata
    23281 acaatgtcct ggcaaaagtc ttattgtgac ggaaaacgaa cgccacgcaa agctgaccgc
    23341 acaaaagggg agtgcttttc tgtgcttagc ggttagaata gtctcatgac tatatctgga
    23401 gttgaccatg ttagagttat taaaaagtct ggtattcgcc gtaatcatgg tacctgtcgt
    23461 gatggccatc atcctgggtc tgatttacgg tcttggtgaa gtattcaaca tcttttctgg
    23521 tgttggtaaa aaagaccagc ccggacaaaa tcattgattc cctgaatgcc cgcttagtcg
    23581 ggcattttct ttttctcaac ttcctgcttt tcctgccgat attttttctt atctacctca
    23641 caaaggttag caataactgc tgggaaaatt ccgagttagt cgttatattg tcgcctacat
    23701 aacgttacat taaggggtta ccaatggctc gtaaatggtt gaacttgttt gccggggcgg
    23761 cactctcttt cgctgttgct ggcaatgcac tggcagatga agggaaaatc acggtgttcg
    23821 ccgccgcatc actgactaac gcaatgcagg acattgctac gcagtataaa aaagagaaag
    23881 gcgtggatgt ggtttcttct ttcgcttcgt catctactct cgcccgtcag attgaagcgg
    23941 gtgcccctgc ggatctgttt atttctgccg atcagaaatg gatggattat gcggttgata
    24001 aaaaagcgat cgatactgct acgcgtcaga cactgctcgg caatagcctg gtcgttgtag
    24061 caccgaaagc cagcgagcag aaagatttca ccatcgacag caaaaccaac tggacttcgc
    24121 tgctgaatgg cggtcgtctg gcggttggcg atccggaaca tgttcccgct ggcatttatg
    24181 caaaagaagc actgcaaaaa ctgggcgcat gggatacgct ctctccgaaa ctggctccgg
    24241 cggaagatgt tcgtggcgcg ctggcgttgg tcgaacgcaa tgaagcgccg ctgggcatag
    24301 tttacggttc tgacgcagtt gccagcaaag gggtaaaagt ggtcgccacc ttcccggaag
    24361 attcgcataa aaaagtggaa tacccggttg ctgttgtgga agggcataac aatgcgacag
    24421 tgaaagcatt ttatgattat ttgaaagggc cgcaggctgc ggaaatcttt aaacgttacg
    24481 gatttacaac caagtaatga tactgaccga tccagaatgg caggcagttt tattaagcct
    24541 gaaagtttct tccctggctg tgctgtttag cctgccgttt gggatctttt ttgcctggtt
    24601 actggtgcgt tgcacgtttc cgggcaaagc tctgctcgac agcgtactgc atctaccgct
    24661 ggtgttaccg cccgtggtcg tcggttactt attattagtt tcgatgggac ggcgcgggtt
    24721 tatcggtgaa cgtctgtatg actggtttgg cattaccttc gcctttagct ggcgcggcgc
    24781 ggttctcgct gccgccgtca tgtcgtttcc gctgatggtg cgggcaattc gtctggcgct
    24841 ggaaggggtt gatgtcaaac tcgaacaggc cgcaagaaca ctgggggccg ggcgctggcg
    24901 cgttttcttt actatcacgt taccgctgac cttaccggga attattgttg gtacggtact
    24961 ggcttttgct cgttctctcg gtgagtttgg tgcaaccatc acctttgtgt cgaacattcc
    25021 tggtgaaacg cgaaccattc cttctgccat gtataccctg atccagaccc ccggcgggga
    25081 aagtggcgcg gcgagactgt gcattatttc tattgcgctg gcgatgatct ccctgttgat
    25141 ttcagaatgg ctggccagaa tcagccgtga acgggcgggg cgctaatcat gctggaactg
    25201 aatttttccc agaccttggg caaccattgc ctgaccatta atgaaacgct gcccgccaat
    25261 ggcatcactg ctatctttgg cgtctccggt gccggaaaaa cttcgctgat taacgccatc
    25321 agtggactga cgcgcccgca aaaagggagg attgtcctca atggtcgggt actaaatgat
    25381 gccgaaaaag gtatctgcct gacgccagaa aagcgtcgcg ttggctatgt ttttcaggat
    25441 gcgcggctgt ttccgcatta caaagtgcgt ggcaatctgc gctacggcat ggcgaaaagc
    25501 atggtcgatc agttcgataa gctggtggcg cttttaggca ttgaaccgtt gcttgaccgt
    25561 ttaccaggca gcctgtccgg aggcgaaaaa cagcgcgtag cgattgggcg ggctttgctg
    25621 acggcaccag aattgctgtt gctggatgaa ccgctggcgt cactggatat tccgcgtaaa
    25681 cgcgaactgt tgccttatct gcaacgtctg acgcgggaaa tcaacatccc gatgttgtat
    25741 gtcagccatt cgctggatga gatcctccat ctggcagaca gagtgatggt actggaaaac
    25801 ggtcaggtga aagcctttgg cgcactggaa gaagtctggg gtagtagcgt gatgaatccg
    25861 tggctgccga aagagcaaca aagtagcatt ctgaaagtga cggtacttga gcatcatccg
    25921 cattacgcga tgactgcgct ggcgctgggc gatcagcatt tgtgggtcaa taagctggac
    25981 gaaccgctgc aagctgcgct acgtatccgc attcaggctt ccgatgtttc tctggtttta
    26041 caaccgccgc agcaaaccag cattcgtaac gtattgcggg caaaagttgt taatagttat
    26101 gacgacaacg gccaggtgga agtggaactg gaagtcggag gtaaaacgct gtgggcgcgt
    26161 atcagcccgt gggccaggga tgaactggtg atcaaacctg gcctgtggct gtacgcgcaa
    26221 attaaaagtg tgtcgataac cgcctgatta aatcaggtgg ctataaatga actgggcaat
    26281 gctgtcggtg gtgttatcac caatcacaat gttggcgcgc gcttttaccg cgtcatcggc
    26341 gttgcccatc gccacgcctg taccagcggc ctccagcata ctgatatcat taaagttatc
    26401 gccgaacgcc acgacgtttt ccatcgacca gccttgcgcc tcaacccatt tcgtcaaacg
    26461 tttacctttg ctgttgccgc cgcgtgcaat atcaacctga tcgtgccagg accattcaca
    26521 ctccagtccc agttcatgtt cgacatgctt accaaaatgc tgcaattgcg gcaggtcatc
    26581 gtgcgtcagg gcgaacttcc atacggcgtt aacttgttgc gccgtttcag ccagagaagc
    26641 gacttgtgtg aaagtcggac gctgttccgg cggcagggtt tgcgcccagt tagacgtgcg
    26701 aatgacatgc ccggtcgggt gctcatagac cattgcatca tccacataca tcagaccgtg
    26761 aatgtggtgt tcattcagca tctcaatgag ttgcagggct ttattaacgg gcattgggtc
    26821 cgcttccagc acggtttttg catgataatc atacaaatag gtgccattac agcaaatagc
    26881 aggtgtatcc agcgccagcg cctgataaaa aggatgaata gcgacgtgat ggcgacctgt
    26941 gacgatgatt aattgatagc ctgcttcgcg agcgcgggcc agggcttcta tcgatgaagg
    27001 aagcagggtc tttttcgggg tcaataaggt gccgtctaag tcgagagcaa tcacgcgtgt
    27061 ggtcatggcg tattccagat taaggttaag aattttcgtc tgcgcgaatg gtacaccgat
    27121 accactatcg gacaaaattc tgcgttttaa ttcagcattc accgccaaaa gcgactaatt
    27181 ttagctgtta cagtcagttg ctaaatgcaa aggagcattc atgaagcaaa cagtttatat
    27241 cgccagccct gagagccagc aaattcacgt ctggaacctg aatcatgaag gcgcactgac
    27301 gctgacacag gttgtcgatg tgccggggca ggtgcagccg atggtggtca gcccggacaa
    27361 acgttatctc tatgttggtg ttcgccctga gtttcgcgtc ctggcgtata gtatcgcccc
    27421 ggacgatggc gcactgacct ttgccgcaga gtctgcgctg ccgggtagtc cgacgcatat
    27481 ttccaccgat caccaggggc agtttgtctt tgtaggttct tacaatgcgg gtaacgtgag
    27541 cgtaacgcgt ctggaagatg gcctgccagt gggcgtcgtc gatgtggtcg aggggctgga
    27601 cggttgccat tccgccaata tctcaccgga caaccgtacg ctgtgggttc cggcattaaa
    27661 gcaggatcgc atttgcctgt ttacggtcag cgatgatggt catctcgtgg cgcaggaccc
    27721 tgcggaagtg accaccgttg aaggggccgg cccgcgtcat atggtattcc atccaaacga
    27781 acaatatgcg tattgcgtca atgagttaaa cagctcagtg gatgtctggg aactgaaaga
    27841 tccgcacggt aatatcgaat gtgtccagac gctggatatg atgccggaaa acttctccga
    27901 cacccgttgg gcggctgata ttcatatcac cccggatggt cgccatttat acgcctgcga
    27961 ccgtaccgcc agcctgatta ccgttttcag cgtttcggaa gatggcagcg tgttgagtaa
    28021 agaaggcttc cagccaacgg aaacccagcc gcgcggcttc aatgttgatc acagcggcaa
    28081 gtatctgatt gccgccgggc aaaaatctca ccacatctcg gtatacgaaa ttgttggcga
    28141 gcagggacta ctgcatgaaa aaggccgtta tgcggtcggg cagggaccaa tgtgggtggt
    28201 ggttaacgca cactaaccgc tgatttaacc ggcgcagtct ctcctgcgcc ggtgtattaa
    28261 cctatctcct gtaacgcatg tctctggcgt tcgacgatat tggtccacaa attgtcttta
    28321 tcgtcagtcc acaaattaat cagcaaggca aaaaagcgtt ctgctgccgg agaaagcacg
    28381 gcatctttgc ggcgaataat ccccaatgtc cgacgtataa cgggttccac cagtggaata
    28441 ccaataatcg aagaataggg cgcatggggc attgccagac caggaagcgc cgaaatcccc
    28501 agtcccgcct ctaccagtcc taatgacgtc gaaagatgac gcacttcgta aaaccagtcc
    28561 agcttccagg gcttgtcggc tagctgttgt tctatcaaca atcggttgcc gctggaggaa
    28621 cgtacgccaa tcattttgta acccactaac tcctgccatt ctacaagctg ctttttggcc
    28681 aatggatggt cacgccgaca ggccagcacg aatggctcgt tgaccagtgg ggtaaaatca
    28741 atggatgaat ttgtgacgtt gttcatgttt atgccaaagt ctgattcgtt acacagcacc
    28801 gattccatgc aattattcgt gccttgttcc agaatccgca ctttaatatt gggatacagc
    28861 tcattaaatt taccgattgc cagcggtaaa aaataaaata ctgcggtcgg aatacacgct
    28921 aacgtcacca taccacgatg ataagcattc atatcacgaa tattaaaaag cgtttcatcg
    28981 aatttcttta ttaattcccg cgcctccggg agcaaccttt ttcctgcttt tgttaacgta
    29041 accttgcgag ttgtacgctc aaatagttga atatgtaaat cttcctccat cttttttatt
    29101 cttcgcgtta atgcaggttg cgtaatattg agtaatttag cagcattgtt aaatgaactg
    29161 gactctgcca gtatgacaaa tgccttcata cttgataatt catgcttcat tattactccg
    29221 gaaaatggaa gcgacgattt tgggtggctg gtcgttaaaa attttaactg catttagcca
    29281 acttaaatta atgaaaaaat gttattaatc gttgagctaa agtcattaga gatgctttgt
    29341 ccttaatgta accatatcgc aataagttat gtttttaaat tgagggcatt attatgaaaa
    29401 aaataccctg cgtgatgatg cgaggtggaa cctcgagggg cgcgttcctg ttagcggaac
    29461 atttacccga agatcaaacg cagcgcgata aaatattgat ggcaattatg ggttccggta
    29521 acgatctgga aattgacggt attggcggcg gtaatccact gaccagtaaa gtcgccatta
    29581 ttagccgttc cagcgatccg cgtgctgatg ttgattatct gtttgctcaa gttatcgtcc
    29641 atgagcaacg tgtcgatacc acgcctaact gcggcaatat gctttctggc gttggggcat
    29701 tcgccattga aaatggtttg attgcagcga catcaccggt cacccgtgtg cgtatccgca
    29761 acgtcaatac gggtacgttc atcgaagctg atgtgcaaac gccaaatggt gttgtcgagt
    29821 acgagggtag tgccagaatt gacggcgtac cgggtactgc cgcaccggtt gcgctcactt
    29881 tcctgaatgc cgctggaacc aaaaccggaa aagttttccc gactgataat cagattgatt
    29941 attttgacga tgtcccggtg acctgtatcg atatggcgat gccagtcgtc attattccgg
    30001 ctgaatatct gggaaaaaca ggttatgaat taccggcgga actggatgcc gacaaagcat
    30061 tattagcccg cattgaatct atccgtctac aagcgggtaa agcaatgggc ttaggtgatg
    30121 tcagtaatat ggttatccct aaacctgtgc ttatttctcc agcgcagaaa ggcggggcaa
    30181 ttaatgtgcg ttattttatg ccgcattctt gccatcgcgc gctggcgata accggtgcta
    30241 ttgctatttc cagtagttgt gcattggaag gcaccgtcac ccgacaaatc gtcccttctg
    30301 taggatacgg caatatcaat attgaacacc ccagtggtgc gctcgacgtt catttaagta
    30361 atgaaggtca ggatgccacg acgttacgcg catctgttat tcggacaacc agaaaaatat
    30421 tttccggtga agtttatctt ccctgaaaaa attcgttgtc aggataagga caatcaataa
    30481 aggacttctg tatgagtcat acagaaagaa caggatttta aatgaataag aaatcgttat
    30541 ggaagctaat tctgatatta gcgatcccat gtattattgg ctttatgcca gctccggcag
    30601 gattaagcga actggcctgg gtgctttttg gtatttacct ggcggccatt gtggggctgg
    30661 ttatcaagcc tttcccggaa cctgtcgtac tgttaattgc cgttgctgcc tcgatggtgg
    30721 tggtcggtaa cttatccgac ggtgcgttta agaccaccgc cgtattaagc ggttactctt
    30781 caggtactac ctggctggtg ttctcggcgt ttaccttaag cgccgcattt gtgaccaccg
    30841 gtttaggtaa acgtattgcc tatctgctga ttggtaaaat cggtaacacc acgctgggtc
    30901 tgggttacgt tacggtattc ctcgatctgg tactggctcc ggcaacaccg tctaacaccg
    30961 cgcgtgcggg cggcattgtg ttaccgatca tcaacagcgt ggcggtggct ttggggtccg
    31021 aaccggaaaa aagtccgcgt cgtgtcggac attacctgat gatgtccatt tacatggtca
    31081 ccaaaaccac cagctatatg ttctttaccg caatggcggg gaacattctg gcgctgaaaa
    31141 tgatcaacga cattctgcac ctgcaaatta gctggggtgg atgggcactg gccgccggat
    31201 tgccgggcat cattatgctg ctggtcaccc cactggtgat ttataccatg tatccgccag
    31261 aaattaagaa ggtggataat aaaaccatcg ctaaagcggg ccttgccgaa ctgggaccga
    31321 tgaaaatccg cgaaaaaatg ctgctcggtg tctttgtgct ggcgctgctg ggctggattt
    31381 tcagtaagtc tctgggggtt gatgaatcca ccgtggcaat cgttgttatg gcgaccatgc
    31441 tgctgctggg tatcgttacc tgggaagacg tggttaaaaa taaaggcggc tggaatacct
    31501 taatctggta cggcggtatt atcggattaa gctccttatt atcgaaagtt aaattcttcg
    31561 aatggttagc tgaagtcttt aaaaataacc tggcatttga tggtcacggt aacgttgctt
    31621 tcttcgttat tattttcctc agcattatcg tgcgttattt cttcgcttcc ggtagtgcct
    31681 atatcgttgc tatgttaccg gtatttgcca tgctggcgaa cgtctccggc gcaccgttaa
    31741 tgttaaccgc gctggcactg ttgttctcca actcctatgg cggcatggtt actcactatg
    31801 gcggcgcggc aggtccggtc atctttggcg tgggttataa cgatattaaa tcctggtggt
    31861 tggtcggtgc ggtactgacg atattaacct tcctggtgca tatcaccctc ggcgtgtggt
    31921 ggtggaatat gctgatcggc tggaacatgc tgtaaatat
//
LOCUS       DAPHDW010000042        31911 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.042, whole genome shotgun sequence.
ACCESSION   DAPHDW010000042 DAPHDW010000000
VERSION     DAPHDW010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.042"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..302
                     /locus_tag="RH057_001923"
     CDS             3..302
                     /locus_tag="RH057_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001170054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128292.1"
                     /translation="MGNGNFWQLFLGKEPYSKKSDFVKAWLLWWAITILVLFILSLTT
                     TLNLRSIGIGLSFGFCQILGRYTMSLGWSKYWGVLGVLPVTNIVLFLILIFFKKR"
     gene            complement(349..1236)
                     /gene="rarD"
                     /locus_tag="RH057_001924"
     CDS             complement(349..1236)
                     /gene="rarD"
                     /locus_tag="RH057_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HDV3128293.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFAFI
                     WVALAIFVMDAIYTQRRKS"
     gene            complement(1288..1755)
                     /locus_tag="RH057_001925"
     CDS             complement(1288..1755)
                     /locus_tag="RH057_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HDV3128294.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            1920..2789
                     /gene="pldA"
                     /locus_tag="RH057_001926"
     CDS             1920..2789
                     /gene="pldA"
                     /locus_tag="RH057_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HDV3128295.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            2922..4751
                     /gene="recQ"
                     /locus_tag="RH057_001927"
     CDS             2922..4751
                     /gene="recQ"
                     /locus_tag="RH057_001927"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HDV3128296.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSKGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            4815..5435
                     /gene="rhtC"
                     /locus_tag="RH057_001928"
     CDS             4815..5435
                     /gene="rhtC"
                     /locus_tag="RH057_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HDV3128297.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTARWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(5497..6117)
                     /gene="rhtB"
                     /locus_tag="RH057_001929"
     CDS             complement(5497..6117)
                     /gene="rhtB"
                     /locus_tag="RH057_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HDV3128298.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            6228..7250
                     /gene="pldB"
                     /locus_tag="RH057_001930"
     CDS             6228..7250
                     /gene="pldB"
                     /locus_tag="RH057_001930"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HDV3128299.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATPTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            7258..8058
                     /gene="yigL"
                     /locus_tag="RH057_001931"
     CDS             7258..8058
                     /gene="yigL"
                     /locus_tag="RH057_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HDV3128300.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGVSKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            8134..9033
                     /gene="bioP"
                     /locus_tag="RH057_001932"
     CDS             8134..9033
                     /gene="bioP"
                     /locus_tag="RH057_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HDV3128301.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRGNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQHNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(8921..9874)
                     /gene="metR"
                     /locus_tag="RH057_001933"
     CDS             complement(8921..9874)
                     /gene="metR"
                     /locus_tag="RH057_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HDV3128302.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALENFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            9992..12253
                     /gene="metE"
                     /locus_tag="RH057_001934"
     CDS             9992..12253
                     /gene="metE"
                     /locus_tag="RH057_001934"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HDV3128303.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALGHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDDVAEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIRQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDVSRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRLG"
     gene            complement(12292..13107)
                     /locus_tag="RH057_001935"
     CDS             complement(12292..13107)
                     /locus_tag="RH057_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV3128304.1"
                     /translation="MATTQQSGFTPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGQLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            13369..14130
                     /gene="udp"
                     /locus_tag="RH057_001936"
     CDS             13369..14130
                     /gene="udp"
                     /locus_tag="RH057_001936"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HDV3128305.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTASSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESQAVKIVVEAARRLL"
     gene            14270..15697
                     /gene="rmuC"
                     /locus_tag="RH057_001937"
     CDS             14270..15697
                     /gene="rmuC"
                     /locus_tag="RH057_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HDV3128306.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            15792..16547
                     /gene="ubiE"
                     /locus_tag="RH057_001938"
     CDS             15792..16547
                     /gene="ubiE"
                     /locus_tag="RH057_001938"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HDV3128307.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            16561..17166
                     /gene="ubiJ"
                     /locus_tag="RH057_001939"
     CDS             16561..17166
                     /gene="ubiJ"
                     /locus_tag="RH057_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HDV3128308.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            17163..18803
                     /gene="ubiB"
                     /locus_tag="RH057_001940"
     CDS             17163..18803
                     /gene="ubiB"
                     /locus_tag="RH057_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HDV3128309.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            18882..19151
                     /gene="tatA"
                     /locus_tag="RH057_001941"
     CDS             18882..19151
                     /gene="tatA"
                     /locus_tag="RH057_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HDV3128310.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            19155..19670
                     /gene="tatB"
                     /locus_tag="RH057_001942"
     CDS             19155..19670
                     /gene="tatB"
                     /locus_tag="RH057_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HDV3128311.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            19673..20449
                     /gene="tatC"
                     /locus_tag="RH057_001943"
     CDS             19673..20449
                     /gene="tatC"
                     /locus_tag="RH057_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HDV3128312.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSPEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            20491..21273
                     /gene="tatD"
                     /locus_tag="RH057_001944"
     CDS             20491..21273
                     /gene="tatD"
                     /locus_tag="RH057_001944"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HDV3128313.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAADLNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(21270..21758)
                     /gene="rfaH"
                     /locus_tag="RH057_001945"
     CDS             complement(21270..21758)
                     /gene="rfaH"
                     /locus_tag="RH057_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HDV3128314.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTINATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPATPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            21925..23418
                     /gene="ubiD"
                     /locus_tag="RH057_001946"
     CDS             21925..23418
                     /gene="ubiD"
                     /locus_tag="RH057_001946"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HDV3128315.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IEQGETAPEGPYGDHTGYYNEVDSFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            23464..24165
                     /gene="fre"
                     /locus_tag="RH057_001947"
     CDS             23464..24165
                     /gene="fre"
                     /locus_tag="RH057_001947"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HDV3128316.1"
                     /translation="MTTLSCKVTSVEAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDIPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHP
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(24357..25520)
                     /gene="fadA"
                     /locus_tag="RH057_001948"
     CDS             complement(24357..25520)
                     /gene="fadA"
                     /locus_tag="RH057_001948"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HDV3128317.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDAQACLVGGVEHMGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSGAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVSGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSVSDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(25530..27719)
                     /gene="fadB"
                     /locus_tag="RH057_001949"
     CDS             complement(25530..27719)
                     /gene="fadB"
                     /locus_tag="RH057_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HDV3128318.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLVEGAKAVLRQAINGDLDWKAKRQPK
                     LEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDVVVEAVVENPKVKKAVLAETEQKVRPNTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDTIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAVVDDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEESIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            27909..29240
                     /gene="pepQ"
                     /locus_tag="RH057_001950"
     CDS             27909..29240
                     /gene="pepQ"
                     /locus_tag="RH057_001950"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HDV3128319.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRCTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            29240..29854
                     /locus_tag="RH057_001951"
     CDS             29240..29854
                     /locus_tag="RH057_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HDV3128320.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            29893..31344
                     /gene="trkH"
                     /locus_tag="RH057_001952"
     CDS             29893..31344
                     /gene="trkH"
                     /locus_tag="RH057_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HDV3128321.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            31356..31901
                     /gene="hemG"
                     /locus_tag="RH057_001953"
     CDS             31356..31901
                     /gene="hemG"
                     /locus_tag="RH057_001953"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HDV3128322.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMIKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         7393 a         8114 c         8745 g         7659 t
ORIGIN      
        1 atatgggaaa tggtaacttt tggcagttgt tcttgggtaa agaaccttac agcaaaaaat
       61 ctgattttgt taaggcatgg ttattgtggt gggctattac catccttgtt ctatttattt
      121 taagcctgac tacgacatta aatttaagaa gtataggtat agggttaagt tttggctttt
      181 gtcaaatcct tggtagatat acgatgtctc taggatggag taagtactgg ggagttttgg
      241 gggtacttcc tgtaacaaat attgttcttt ttcttatctt aatttttttc aaaaaaagat
      301 aaataagtca gtgggtaggc tcattaaacg gcctacccat gttaattctt aactttttct
      361 acgctgcgta taaatcgcat ccatcacaaa aattgccagc gccacccaaa taaaggcgaa
      421 agtcaccatc ttatcggcac ccggtttttc accataaaac gtcacagcca gcaggaacat
      481 cagcgtcggg ccaatgtact ggaaaaagcc taacgttgag agacgcaggc gcgtggcagc
      541 ggcagtaaaa cacaacagcg gtacggtagt gacaataccg gcggcgatca gcagtaaatt
      601 cagcgacatc gggttttgcc ccatatggct ggttgagctg tcggcaatag caaacaggta
      661 aatcgccgcc acgggcagca gccacatggt ttcaattaac atgccggttt gtgcttctac
      721 cgcaatcttc ttgcgcacca ggccgtagaa ggcaaaacta aatgccagcc ccagcgcgat
      781 aataggtagc gaaccaaaag tccacagctg gaccaacacg ccacatatcg ccagaatcac
      841 cgccagccat tgcatccggc ggaatcgctc gccgaggaaa atcatcccca gcacaatgtt
      901 caccagcggg ttaataaagt aaccaaggct cgcttccagc atatggtgat tgttcaccgc
      961 ccagataaac agtagccagt tgccaccaat cagcacggca gagactgcca gcataaaaat
     1021 tttctgtggc gtctgaatca gcgtttttaa ataggaccac tggcggcaaa tgctcatcag
     1081 caccaccata aagaaaaacg accagatcac gcgatgcgtc aggatttcat cggcgggcac
     1141 gtagtaaatc aacttgaagt acgctggcgc aataccccaa ataaaataag cggcaagagc
     1201 gagtaatacg ccctgccgcg tttgttttgc atccatcggg aatactcatt tttaattggt
     1261 aacagcagtt tacctgcttt tatgtcttca acctaccata taggtggcgg tggcactggc
     1321 aatataaagc tgttcttcat tgtgtaattc aacgcgggcg acggcgactt tattgcctgc
     1381 acgcaacagg ctactggtag cagtaaaacg ctcgcccctg cctgggcgca gataatcaac
     1441 gcgaagatca atggtcccca tccgcgatag ccgctggcgt agttcatctt cactgatggt
     1501 ttcgtggcgg gttaaggtac ttcccacgca caccagaccg gcggcgacat ccagcgccga
     1561 cgcaatgacc ccgccgtgca aaatgctttg cgcccagttg cccaccatca ttggctgatt
     1621 tttaaaggcc agctgtgcga actctttttc gtaacgctcc agttccatcc ccaatgcgcg
     1681 gttaaatggc atgtgataaa caaacatctc acccactaat ttcagggctt gttcagcagt
     1741 cagtacggca gacatatcat ccttacactt cattggttaa tgaaatgttg attttatgct
     1801 tctttgttgt tggtttctac tttaggaagg gataactaac ggcaatggag ttaagtatgt
     1861 aaaatagccc gcagaaaaac attcacctta tcaataattc gttacggaga acacgaccga
     1921 tgcggactct gcagggctgg ttgttgccgg tgtttatgtt gcctatggca gtatatgcac
     1981 aagaggcaac ggtgaaagag gtgcatgacg cgccagcggt gcgtggcagt attatcgcca
     2041 atatgctgca ggagcatgac aatccgttca cgctctatcc ttatgacacc aactacctca
     2101 tctacaccca aaccagcgat ctgaacaaag aagcgattgc cagttacgac tgggcggaaa
     2161 atgcgcgtaa ggatgaagta aagtttcagt tgagcctggc atttccgctg tggcgtggga
     2221 ttttaggccc gaactcggtg ttgggtgcgt cttatacgca aaaatcctgg tggcaactgt
     2281 ccaatagcga agagtcttca ccgtttcgtg aaaccaacta cgaaccgcaa ttgttcctcg
     2341 gttttgccac cgattaccgt tttgcaggtt ggacgctgcg cgatgtggag atggggtata
     2401 accacgactc taacgggcgt tcagacccga cctcccgcag ctggaaccgc ctttatactc
     2461 gcctgatggc agaaaacggt aactggctgg tagaagtgaa gccgtggtat gtggtgggta
     2521 atactgacga taacccggat atcaccaaat atatgggtta ctaccagctt aaaatcggct
     2581 atcacctcgg tgatgcggtg ctcagtgcga aaggacagta caactggaac accggctacg
     2641 gcggcgcgga gttaggctta agttacccga tcaccaaaca tgtgcgcctt tatactcagg
     2701 tttacagcgg ctatggcgaa tcgctcatcg actataactt caaccagacc cgtgtcggtg
     2761 taggagttat gctaaacgac ttgttttgat gaacggttga gtggttggca aatctggaac
     2821 ccagcatcca ggattaccct ctcagagact aaaagcattg cagtttctcg cgcaggcgct
     2881 gaaaatagcg cctgttttta tttcaggcaa tcggggtgaa tgtggcgcag gcggaagtgt
     2941 tgaatctgga gtccggagct aaacaggttt tacaagaaac ctttggctac caacagtttc
     3001 gccccggcca ggaagaaatt atcgacactg tgctttccgg tcgcgattgc ctggtcgtca
     3061 tgcccaccgg tggcggaaaa tccctttgct atcaaattcc tgccttattg ctaaacggcc
     3121 ttaccgtggt tgtttcaccg ctgatttcgt tgatgaaaga tcaggtggat caactgcaag
     3181 ccaacggcgt ggcggcggcg tgccttaact cgacgcaaac ccgcgaacag caacttgaag
     3241 tgatgacagg ctgccgcacc gggcaaattc gtctgcttta tatcgccccg gaacgcctga
     3301 tgctggataa ctttcttgag catctggcgc actggaatcc ggtgttatta gccgttgatg
     3361 aagcgcactg tatctcccaa tggggccacg atttccgccc ggaatatgcc gcgctcggtc
     3421 agttgcgcca gcggttcccg acgctgccgt ttatggcgct gaccgccaca gccgacgaca
     3481 ccacgcgcca ggatatcgtg cgcctgctgg ggctgaacga tccgctgatt caaatcagca
     3541 gttttgaccg tccgaatatt cgctacatgc tgatggagaa gttcaaaccg ctcgatcagt
     3601 tgatgcgcta cgtgcaggaa cagcgcggta agtccggcat tatctactgc aacagccggg
     3661 cgaaagtaga agacaccgct gcgcgcctgc aaagcaaggg tattagcgcg gcggcctatc
     3721 atgccgggct ggaaaataat gtccgcgccg acgtgcagga gaaattccag cgcgatgacc
     3781 tgcaaattgt ggtggcgacg gtggcgttcg gcatgggcat caataaacca aacgttcgct
     3841 tcgtggtcca ctttgatatt ccgcgcaata tcgaatccta ttatcaggaa accggtcgcg
     3901 ccgggcgtga tggcctgcct gcggaagcga tgctgtttta cgatccggct gatatggcgt
     3961 ggctgcgccg ttgtctggaa gagaagccgc aggggcagtt gcaggatatc gagcgccaca
     4021 aactcaatgc gatgggcgcg tttgccgaag cgcaaacttg ccgtcgtctg gtattgctga
     4081 actattttgg cgaagggcgt caggagccgt gcgggaactg cgatatctgc ctcgatccgc
     4141 cgaaacagta cgacggttca accgatgctc agattgccct ttccaccatt ggtcgtgtga
     4201 atcagcggtt tgggatgggt tatgtggtgg aagtgattcg tggtgctaat aaccagcgta
     4261 tccgcgacta tggtcatgac aaactgaaag tctatggcat gggccgtgat aaaagccatg
     4321 aacattgggt gagcgtgatc cgccagctga ttcacctcgg cctggtgacg caaaatattg
     4381 cccagcattc tgccctacaa ctgacagagg ccgcgcgccc ggtgctgcgc ggcgaatcct
     4441 ctttgcaact tgccgtgccg cgtatcgtgg cgctcaaacc gaaagcgatg cagaaatcgt
     4501 tcggcggcaa ctatgatcgc aaactgttcg ccaaattacg caaactgcgt aaatcgattg
     4561 ccgatgaaag caatgtcccg ccgtacgtgg tgtttaacga cgcaaccttg attgagatgg
     4621 ctgaacagat gccgatcacc gccagcgaaa tgctcagcgt taacggcgtt gggatgcgca
     4681 agctggaacg ctttggtaaa ccgtttatgg cgctgattcg tgcgcatgtt gatggcgatg
     4741 acgaagagta gtcagcagca taaaaaagtg ccaggatgaa gactccgtaa acgtttcccc
     4801 cgcgagtcaa atgtatgttg atgttatttc tcaccgtcgc catggtgcac attgtggcgc
     4861 ttatgagccc cggtcccgat ttcttttttg tctctcagac cgctgtcagt cgttcccgta
     4921 aagaagcgat gatgggcgtg ctgggcatta cctgcggcgt aatggtttgg gctgggattg
     4981 cgttgcttgg cctgcatttg attatcgaaa aaatggcctg gctgcatacg ctgattatgg
     5041 ttggcggtgg cctgtatctc tgctggatgg gttaccagat gctgcgcggc gcgctgaaaa
     5101 aagaggcagt ttctgcacct gcgccacagg tcgagctggc gaaaagtggg cgcagtttcc
     5161 tgaaaggttt actgaccaat ctcgctaatc cgaaagcgat tatctacttt ggctcggtgt
     5221 tctcattgtt tgtcggtgat aacgttggca ctaccgcgcg ctggggcatt tttgcgctga
     5281 tcattgtcga aacgctggcg tggtttaccg tcgttgccag cctgtttgcc ctgccgcaaa
     5341 tgcgccgtgg ttatcaacgt ctggcgaagt ggattgatgg ttttgccggg gcgttatttg
     5401 ccggatttgg cattcatttg attatttcgc ggtgatgcca gacgcgtctt cagagtaagt
     5461 cggataaggc gtttacgccg catccgacat tatttttcac gcatgcctcg ccgatgctaa
     5521 cagcgctccc accagcataa acaacgagcc gaaaatctta ttcagcgcct tcatctgctt
     5581 tggtccttta atccatagag caatccgttg agcaagggtg gcgtaaccga tcatcacaat
     5641 aatatcgacc acaatagtgg tgacgccgag cacgatatac tgcatcagtt gcggctgttg
     5701 cggcatgatg aattgcggaa atagcgccgc cagaaacaca atacttttgg gattggtgag
     5761 attcacaaaa actgcgcgct ggaacaaatg tcgacgcgat tgagtagagg ccagcgattt
     5821 aaggtcaatt gcaccagcgg cgcgccactg ctggattccc agccaaatca agtaagccgc
     5881 gcctgcccac ttcaacactt caaacgcaat cactgagcgg gaaaatagcg tccccaaccc
     5941 cacgccaacc agcacaatat gaatcgccag tccggtctga agcccagcaa tagacgccac
     6001 cgcgccgcga taaccgtggt tgagcgaggt ggtcatagtg ttgattgcac cagagcctgg
     6061 cgacaggctt aaaatgatcg atgtcagcag gtaggcaaac caccattcta aggtcatgat
     6121 gaactcccgg tgtgtctatt tttgtgccac aatacgctac tgtcgcagcg ttgtgtcagg
     6181 cacgctaaaa aaaacgattt tacgtggttt aagaggcaga ttacccgatg tttcagcagc
     6241 aaaaagactg ggaaacaaga gaaaacgcgt ttgctgcttt taccatggga ccgctgactg
     6301 atttctggcg tcagcgtgat gaagcagagt ttactggtgt ggatgacatt ccggtgcgct
     6361 ttgtccgttt tcgcgcacag caccatgacc gggtggtagt catctgcccg gggcgtattg
     6421 agagctacgt aaaatatgcg gaactggcct atgacctgtt ccatttgggg tttgatgtct
     6481 taatcatcga ccatcgcggg cagggacgtt ccggtcgcct gttagccgat ccgcatctcg
     6541 ggcatgttaa tcgctttaat gattatgttg atgatctggc ggcattctgg cagcaggagg
     6601 ttcagcccgg tccgtggcgt aaacgctata tactggcaca ttcgatgggc ggtgcgatct
     6661 ccacattatt tctgcaacgc catccaggtg tatgtgacgc cattgcgcta actgcgccaa
     6721 tgtttgggat cgtgattcgt atgccgtcat ttatggcacg gcagatcctc aactgggccg
     6781 aagcgcatcc acgtttccgt gatggctatg caataggcac cgggcgctgg cgcgcgttgc
     6841 cgtttgctat caacgtactg acccacagca gacagcgata tcgacgtaac ttacgcttct
     6901 atgctgatga cccaacgatt cgcgtcggtg ggccgaccta ccattgggta cgcgaaagta
     6961 ttctggctgg cgaacaggtg ttagccggtg cgggtgatga cgccacgcca acgcttctct
     7021 tgcaggctga agaggaacgc gtggtggata accgcatgca tgaccgtttt tgtgaactcc
     7081 gcaccgccgc gggccatcct gtcgaaggag gacggccgtt ggtaattaaa ggtgcttacc
     7141 atgagatcct ttttgaaaag gacgcaatgc gctcagtcgc gctccacgcc atcgttgatt
     7201 ttttcaacag gcataactca cccagcggaa accgctctac agaggtttaa atttcttatg
     7261 taccaggttg ttgcgtctga tttagatggc acgttacttt ctcccgacca tacgttatcc
     7321 ccttacgcca aagaaactct gaagctgctc accgcgcgcg gcatcaactt tgtgtttgcg
     7381 accggtcgtc accacgttga tgtggggcaa attcgcgata atctggagat taagtcttac
     7441 atgattacct ccaatggtgc gcgcgttcac gatctggatg gtaatctgat ttttgctcat
     7501 aacctggatc gcgacattgc cagcgatctg tttggcgtag tcaacgacaa tccggacatc
     7561 attactaacg tttatcgcga cgacgaatgg tttatgaatc gccatcgccc ggaagagatg
     7621 cgctttttta aagaagcggt gttcaaatat gcgctgtatg agcctggatt actggagccg
     7681 gaaggcgtca gcaaagtgtt cttcacctgc gattcccatg aacaactgct gccgctggag
     7741 caggcgatta acgctcgttg gggagatcgc gtcaacgtca gtttctctac cttaacctgt
     7801 ctggaagtga tggcgggcgg cgtttcaaaa ggccatgcgc tggaagcggt ggcgaagaaa
     7861 ctgggctaca gcctgaagga ttgtattgcg tttggtgacg ggatgaacga cgccgaaatg
     7921 ctgtcgatgg cggggaaagg ctgcattatg ggcagtgcgc accagcgtct gaaagacctt
     7981 catcccgagc tggaagtgat tggtactaat gccgacgacg cggtgccgca ttatttgcgt
     8041 aaactctatt tatcgtaatc gttctttatt tggtcagttg tcaacctgat acttcgctac
     8101 aatggatact cgttaatcaa agagttttcc attgtggcgc tacttatcat caccacgatt
     8161 ctgtgggcct tctcctttag cttttatggc gagtaccttg cggggcacgt cgatagctat
     8221 tttgcggtgc tggtgcgcgt tggcctggcg gcactcgttt ttctgccgtt tctgcgtacc
     8281 cgtggcaata gcctgaaaac agtcggcctg tatatgctgg tgggcgcgat gcagcttggc
     8341 gtgatgtata tgctgagttt ccgcgcttat ctctatctga cggtttccga actgctgctg
     8401 ttcaccgtac tgacgccgct ctacatcacg ctgatttatg acatcatgag taagcgccgt
     8461 ctgcgctggg gatatgcttt tagtgccttg ttggcggtga ttggtgccgg gattattcgc
     8521 tatgatcagg tcaccgacca tttctggact ggcttgttgc tggtgcaact ctccaatatc
     8581 acttttgcca tcggcatggt gggttacaaa cgcctgatgg aaactcgccc gatgccgcag
     8641 cataacgcct ttgcgtggtt ctatcttggc gcgttcctgg tggcggtaat tgcatggttc
     8701 ttgctgggaa atgcgcagaa aatgccgcaa accacgttgc aatggggcat tctggtgttt
     8761 cttggcgtgg tggcttccgg gattggctac tttatgtgga actacggcgc gacgcaggtg
     8821 gacgccggaa cgctgggaat tatgaataat atgcacgttc cggcagggct gctggtaaac
     8881 ctggctatct ggcaccaaca gccgcactgg ccaacgttta ttacaggcgc gctggtgatc
     8941 ctggcctcac tgtgggtgca tcgtaagtgg gtcgctccgc gctcttcaca aacggcagat
     9001 gatcgcaggc gtgattgcgc gctgagcgaa taaacgcttc cgtaactggc tgacgctgct
     9061 cgccatcgcg cacggcggcg tacagtcggc tccacaagcc ttcgcccagg gttttggtca
     9121 ccaccagacc ctggcgctca aaactctcta ctacccaatg cggtagcgcg gcaataccca
     9181 tccgcgcggc aaccatctga atcaacaata aggtgttatc gacgcttttt agtgacggac
     9241 tgacgcctgc cggctgaaga aaatgccgcc agacatccag tcgactacgc tgcaccggat
     9301 aaattaatag cgtctcgctg gcgagatctt ccggtgtaat acgcgttttt gccgccagtg
     9361 gatggtcagg tgctaacacc agacgcactt catagtcgaa catcggcgaa taatgcagac
     9421 cactgcgcgg cagaatatcg gacgtcatta ccagatccag ctcgccctgt tgcaacgcgg
     9481 gctgcgggtc aaacgtcacg cccgatttaa aatccatctc tacctgcggc cagttcttat
     9541 ggaaattttc taacgcgggc gtcagccact gaatacagct atggcactca atggcaatgc
     9601 gcagacgcgt ctgctgcggt tcattgcagg cttgcagtgc ctggctaatt tgcggcagca
     9661 cctggtttgc cagttgcaac aggatttctc cctgcggtgt aaagcgtagc ggctggctct
     9721 tacgcacaaa tagccggaag ccaaggcgtt gttccagatc gctaaactgg tgagacaggg
     9781 cggattgcgt ctgatgcaac gtcgccgcag cggctgcgag agagccgcag ttccgcaacg
     9841 cttgtagcgt tttcaggtgt tttacttcga tcatgaaagt ccttcacttc ggcatgaata
     9901 atttgcgctt gaggaatata cagtaaccgc caattatgga tgtgtaaaca tctggacggc
     9961 taaatactaa aaattccata aggggcatat aatgacaatt cttaatcaca ccctcggttt
    10021 ccctcgcgtt ggcctgcgtc gcgagctgaa aaaagcgcaa gaaagttatt gggcggggaa
    10081 ctccacgcgt gaagaactgc tgacagtagg gcgtgaactg cgtgctcgtc actgggatca
    10141 acaaaagcaa gcgggtatcg acctgctgcc ggtgggcgat tttgcctggt acgatcatgt
    10201 tctgaccacc agtctgctgc tgggtaatgt tccgccacgt catcagaaca aagacggttc
    10261 ggtagatatc gacaccctgt tccgtattgg tcgtggacgt gcgccgactg gcgaacctgc
    10321 ggcggcagcg gaaatgacca aatggtttaa caccaactat cactacatgg tgccggaatt
    10381 cgttaaaggc caacagttca aactgacctg gacgcagctg ctggaggaag tggacgaggc
    10441 gctggcgctg ggccacaacg ttaagcctgt gctgctgggg ccggttactt acctgtggct
    10501 ggggaaagtg aaaggtgaac agtttgaccg ccttagcctg ctgaacgaca ttctgccggt
    10561 ttatcagcaa gtgctggcag aactggcgaa acgcggcatc gagtgggtac agattgatga
    10621 acccgcgctg gtactggaac taccacaggc gtggctggac gcatacaaac ccgcttacga
    10681 cgcgctccag ggacaggtga aactgctgct gaccacctat tttgaaggcg taacgccaaa
    10741 tctcgacacg attactgcgc tgcctgttca gggtctgcat gttgacctcg tacatggtaa
    10801 agatgacgtt gctgaactgc acaagcgcct gccttctgac tggttgctgt ctgcgggtct
    10861 gatcaatggt cgtaacgtct ggcgcgccga tcttaccgag aaatatgcgc aaattaagga
    10921 cattgtcggc aaacgtgatt tgtgggtggc atcttcctgc tcgttgctgc acagccccat
    10981 cgacctgagc gtggaaacgc gtcttgatgc agaagtgaaa agctggtttg ccttcgccct
    11041 acaaaaatgc catgaactgg cactgctgcg cgatgcgctg aacagtggtg acacggcagc
    11101 tctggcagag tggagcgccc cgattcaggc acgtcgtcac tctacccgcg tacataatcc
    11161 ggcggtagaa aagcgtctgg cggcgatcac cgcccaggac agccagcgtg cgaatgtcta
    11221 tgaagtgcgt gccgaagccc agcgtgcgcg ttttaaactg ccagcgtggc cgaccaccac
    11281 gattggttct ttcccgcaaa ccacggaaat tcgtaccctg cgtctggatt tcaaaaaggg
    11341 caatctcgac gccaataact accgcacggg cattgcggaa catatcaggc aggccattgt
    11401 tgaacaggaa cgtttgggac tggatgtgct ggtacatggc gaggccgagc gtaatgacat
    11461 ggtggaatac tttggcgagc acctcgacgg gtttgtcttt acgcaaaacg gttgggtaca
    11521 gagctacggt tcccgctgcg tgaagccacc gattgttatt ggtgacgtta gtcgcccggc
    11581 accgattacc gtggagtggg cgaagtatgc gcaatcgctg accgacaaac cggtgaaagg
    11641 gatgctgacg gggccggtga ccatactctg ctggtcgttc ccgcgtgaag atgtcagccg
    11701 tgaaaccatc gccaaacaga ttgcgctggc gctgcgtgat gaggtggccg atctggaagc
    11761 ggcagggatt ggtatcatcc agattgacga accggcgctg cgcgaaggtt taccactgcg
    11821 tcgtagcgac tgggacgcct atctccagtg gggcgtagag gccttccgta tcaacgccgc
    11881 cgtggcgaaa gatgacacac aaatccacac tcacatgtgt tattgcgagt tcaacgacat
    11941 catggattcc attgcggcgc tggatgcaga cgtcatcacc atcgaaacct cgcgttccga
    12001 catggagttg ctggagtcgt ttgaagagtt tgattatccg aatgaaatcg gtccaggcgt
    12061 ctatgacatt cactcgccaa acgtaccgag cgtggaatgg attgaagccc tgcttaagaa
    12121 agcggcaaaa cgcattccgg cagagcgcct gtgggtcaac ccagactgtg gcctgaaaac
    12181 gcgcggctgg ccagaaaccc gcgcggcact ggcgaacatg gtgcaggcgg cgcagaattt
    12241 gcgtctgggg taaatccaaa ccgggtggta ataccacccg gtcttttctc attacagcga
    12301 cttcttccca ccatactgct taaaccattc cagcatacgc tgccagccat ctttcgcaga
    12361 ttcggcatga tagctcgggc gataatcagc gttgaatgca tgcccggcat ccgggtagac
    12421 gataatctct gctttcgcat tagcagcccg cagcgcctgg cgcatcgttt caacgctctc
    12481 ctgcggaatg ctgttatcct gaccgccata taagccgaga accggcgcgt taagatcggt
    12541 tgcgatatca acaggttgtt tcggtgaatt cagcgactta tcgcccgtca gtttgccgta
    12601 ccacgccact gcggctttta gctgtggatt atgcgcggca tacagccagg tgatacgtcc
    12661 accccagcag aatccggtga tcattaaacg atgaacatcg ccgccgttgc gggaagccca
    12721 actggcgaca tgatcgagat cggccagcac ctgcgagtca ggcacttttg ctaccagacc
    12781 gctaagcaac gtggggatat cggcaaaatc attcggatcg ccttcgcgga agtaaagttc
    12841 aggtgcgata gccagatacc cctccagcgc cagacggcga caaatgtcgc ggatatgttc
    12901 atgcacgcca aaaatttcct gcactacaat gaccactggc agttggccat cgctttgctt
    12961 tggtctggca tggtaagcag gcatgttatc cccttgtgaa gggatagagg tgaagcccgc
    13021 cacaatcgcg tcgtccgggg tctgaacgat ggtcgaagcg agaggcgatg cagcaggtgt
    13081 aaatccagat tgttgtgttg ttgccatggt attctccgta cccttataaa aatgttgcgc
    13141 aatgttaact atagtcagca tgcaacaaat cacattgcct gaatcggctc atcttttatg
    13201 cagtcctgca gaatgaagag tgatttatgt gatttgaatc acttttggtg ggtaaattta
    13261 tgcaacgcat ttgcgccatg gtgatgagta tcacgaaaaa atgttaaacc cttcggtaaa
    13321 gtgtcttttt gcttcttctg actaaaccga ttcacagagg agttgtatat gtccaagtct
    13381 gatgtttttc atctcggcct cactaaaaac gatttacaag gggctacgct tgccatcgtc
    13441 cctggcgacc cggatcgtgt ggaaaagatc gccgcgctga tggataagcc ggttaagctg
    13501 gcatctcacc gcgaattcac tacctggcgt gcagagctgg atggtaaacc tgttatcgtc
    13561 tgctctaccg gtatcggcgg cccgtctacc tctattgctg ttgaagagct ggcacagctg
    13621 ggcattcgca ccttcctgcg tatcggtaca acgggcgcta ttcagccgca tattaatgtg
    13681 ggtgatgtcc tggttaccac ggcgtctgtc cgtctggatg gcgcgagcct gcacttcgca
    13741 ccgctggaat tcccggctgt cgctgatttc gaatgtacga ctgcgctggt tgaagctgcg
    13801 aaatctattg gcgcgacaac tcacgttggc gtgacagctt cttctgatac cttctaccca
    13861 ggtcaggaac gttacgatac ttactctggt cgcgtagttc gtcactttaa aggttctatg
    13921 gaagagtggc aggcgatggg cgtaatgaac tatgaaatgg aatctgcaac tctgctgacc
    13981 atgtgtgcaa gccagggcct gcgtgccggt atggtggcgg gcgttatcgt taaccgcacc
    14041 cagcaagaga tcccgaatgc tgagacgatg aaacaaaccg aaagccaagc ggtgaaaatc
    14101 gtggtggaag cggcgcgtcg tctgctgtaa tcctcttctc ctgtctgaag gccgacgcgt
    14161 tcggcctttt gtatttttgc gtagcgcctc gcaggaaatg cctttccaac tggacgtttg
    14221 tacagcacaa ttctatgttg tgcgggtaag taatgaggct ggaggcgttg tggatttctc
    14281 aatcatggtt tacgcagtta ttgcgttggt gggtgtggca attggctggc tgtttgccag
    14341 ttatcaacat gcgcagcaaa aagccgagca attagctgaa cgtgaagaga tggtcgcgga
    14401 gttaagcgcg gcaaaacaac aaattaccca aagcgagcac tggcgtgcag agtgtgaatt
    14461 gctcaataac gaagtgcgca gcctgcaaag tattaacacc tctctggagg ccgatctgcg
    14521 tgaagtaacc acgcggatgg aagccgcaca gcaacatgct gacgataaaa ttcgccagat
    14581 gattaacagc gagcagcgcc tcagtgagca gtttgaaaac ctcgctaacc gtatttttga
    14641 gcacagcaat cgccgggttg atgagcaaaa ccgtcagagt ctgaacagcc tgttgtcgcc
    14701 gctacgtgaa caactggacg gtttccgccg tcaggttcag gacagcttcg gtaaagaagc
    14761 acaagaacgc cataccctga cccacgaaat tcgtaatcta cagcaactca acgcgcaaat
    14821 ggcccaggaa gcgatcaacc tgacgcgcgc gctgaaaggc gataacaaaa cccaggggaa
    14881 ctggggtgaa gtggtactga cgcgggtgct ggaggcttcc ggtctgcgtg aagggtatga
    14941 atatgaaacc caggtcagca tcgaaaatga cgcccgctcg cggatgcagc cggatgtcat
    15001 cgtgcggttg ccgcagggaa aagatgtggt gatcgacgcc aaaatgacgc tggtcgccta
    15061 tgaacgctat ttcaacgccg aagacgacta cacccgcgaa agcgcgctac aggaacatat
    15121 cgcgtcggtg cgtaaccata tccgtttgct gggacgcaaa gattatcaac agctgccggg
    15181 gctgcgaact ctggattacg tgctgatgtt tattcccgtt gaacccgctt ttttactggc
    15241 gcttgaccgc cagccggagc tgatcaccga agcgttgaaa aacaatatca tgctggttag
    15301 cccgactacg ctgctggtgg cgttgcgtac tattgccaac ctgtggcgtt atgagcatca
    15361 aagccgcaac gcccagcaaa tcgccgatcg tgccagcaag ctgtacgaca agatgcgttt
    15421 gttcatcgat gacatgtccg cgattggtca aagtctcgac aaagcgcagg ataactaccg
    15481 ccaggcaatg aaaaaactct cttcaggtcg cggaaatgtg ctggcgcagg cagaagcgtt
    15541 tcgcggttta ggagtagaaa ttaaacgcga gattaatccg gatttggctg aacaggcggt
    15601 gagccaggat gaagagtatc gacttcggtc ggttccggag cagccgaatg atgaagctta
    15661 tcaacgcgat gatgaatata atcagcagtc gcgctagccc attaggggta gttaagccgg
    15721 gtagaaatct agggcatcga cgcccaatct gttacacttc tggaacaatt ttttgatgag
    15781 caggcattga gatggtggat aagtcacaag aaacgacgca ctttggtttt cagaccgtcg
    15841 cgaaggaaca aaaagcggat atggtcgccc acgttttcca ttccgtggca tcaaaatacg
    15901 atgtcatgaa tgatttgatg tcatttggta ttcatcgttt gtggaagcga ttcacgattg
    15961 attgcagcgg cgtacgccgt gggcagaccg tgctggatct ggctggtggc accggcgacc
    16021 tgacagcgaa attctcccgc ctggtcggag aaactggcaa agtggtcctt gctgatatca
    16081 atgaatccat gctcaaaatg ggacgcgaga agctgcgtaa tatcggtgtg attggcaacg
    16141 ttgagtatgt tcaggcgaac gctgaggcgc tgccgttccc ggataacacc tttgattgca
    16201 tcaccatttc gtttggtctg cgtaacgtca ccgacaaaga taaagcactg cgttcaatgt
    16261 atcgcgtgct gaaacccggc ggccgcctgc tggtgcttga gttctcgaag ccaattatcg
    16321 agccgctgag caaagcctat gatgcatact ccttccatgt gctgccgcgt attggctcac
    16381 tggtcgcgaa cgacgccgac agctaccgtt atctggcaga atccatccgt atgcatcccg
    16441 atcaggatac cctgaaagcc atgatgcagg atgccggatt cgaaagtgtc gactactaca
    16501 atctgacggc aggggttgtg gcgctgcatc gtggttataa gttctgacag gagaccggaa
    16561 atgcctttta aacctttagt gacggcagga attgaaagtc tgctcaacac cttcctgtat
    16621 cgctcacccg cgctgaaaac agcccgctcg cgtctgctgg gtaaagtatt gcgcgtggag
    16681 gtaaaaggct tttcgacgtc attgattctg gtgttcagcg aacgccaggt tgatgtactg
    16741 ggcgaatggg caggcgatgc tgactgcacc gttatcgcct acgccagtgt gttgccgaaa
    16801 cttcgcgatc gccagcagct taccgcactg attcgcagtg gtgagctgga agtgcagggc
    16861 gatattcagg tggtgcaaaa cttcgttgcg ctggcagatc tggcagagtt cgaccctgcg
    16921 gaactgctgg ccccttatac cggtgatatc gccgctgaag gaatcagcaa agccatgcgc
    16981 ggaggcgcaa agttcctgca tcacggcatt aagcgccagc aacgttatgt ggcggaagcc
    17041 attactgaag agtggcgtat ggcacccggt ccgcttgaag tggcctggtt tgcggaagag
    17101 acggctgccg tcgagcgtgc tgttgatgcc ctgaccaaac ggctggaaaa actggaggct
    17161 aaatgacgcc aggtgaagta cggcgcctat atttcatcat tcgcactttt ttaagctacg
    17221 gacttgatga actgatcccc aaaatgcgta tcaccctgcc gctacggcta tggcgatact
    17281 cattattctg gatgccaaat cggcataaag acaaactttt aggtgagcga ctacgactgg
    17341 ccctgcaaga actggggccg gtttggatca agttcgggca aatgttatca acccgccgcg
    17401 atctttttcc accgcatatt gccgatcagc tggcgttatt gcaggacaaa gttgctccgt
    17461 ttgatggcaa gctggcgaag cagcagattg aagctgcaat gggcggcttg ccggtagaag
    17521 cgtggtttga cgattttgaa atcaagccgc tggcttctgc ttctatcgcc caggttcata
    17581 ccgcgcgatt gaaatcgaat ggtaaagagg tggtgattaa agtcatccgc ccggatattt
    17641 tgccggtcat taaagcggat ctgaaactta tctaccgtct ggctcgctgg gtgccgcgtt
    17701 tgctgccgga tggtcgccgt ctgcgcccaa ccgaagtggt gcgcgagtac gaaaagacct
    17761 tgattgatga actgaatttg ctgcgggaat ctgccaacgc cattcagctt cggcgcaatt
    17821 ttgaagacag cccgatgctc tacatcccgg aagtttaccc tgactattgt agtgaaggga
    17881 tgatggtgat ggagcgcatt tacggcattc cggtgtctga tgttgcggcg ctggagaaaa
    17941 acggcactaa catgaaattg ctggcggaac gcggcgtgca ggtgttcttc actcaggtct
    18001 ttcgcgacag ctttttccat gccgatatgc accctggcaa catcttcgta agctatgaac
    18061 acccggaaaa cccgaaatat atcggcattg attgcgggat tgttggctcg ctaaacaaag
    18121 aagataaacg ctatctggca gaaaacttta tcgccttctt taatcgcgac tatcgcaaag
    18181 tggcagagct acacgtcgat tctggctggg tgccaccaga taccaacgtt gaagagttcg
    18241 aatttgccat tcgtacggtc tgtgaaccta tctttgagaa accgctggcc gaaatttcgt
    18301 ttggacatgt actgttaaat ctgtttaata cggcgcgtcg cttcaatatg gaagtgcagc
    18361 cgcaactggt gttactccag aaaaccctgc tctatgtcga aggggtagga cgccagcttt
    18421 atccgcagct cgatttatgg aaaacagcga agcctttcct ggagtcgtgg attaaagatc
    18481 aggtcggtat tcctgcgctg gtgagagcat ttaaagaaaa agcgccgttc tgggtcgaaa
    18541 aaatgccaga actgcctgaa ttggtttacg acagtttgcg ccagggcaag tatttacagc
    18601 acagtgttga taagattgcc cgcgagcttc agtcaaatca tgtacgtcag ggacaatcgc
    18661 gttattttct cggaattggc gctacgttag tattaagtgg cacattcttg ttggtcagcc
    18721 gacctgagtg ggggctgatg cccggctggt taatggcagg tggtctgatc gcctggtttg
    18781 tcggttggcg caaaacacgc tgattttttc atcgctcaag gcgggccgtg taacgtataa
    18841 tgcggctttg tttaatcatc atctaccaca gaggaacatg tatgggtggt atcagtattt
    18901 ggcagttatt gattattgcc gtcatcgttg tactgctttt tggcaccaaa aagctcggct
    18961 ccatcggttc cgatcttggt gcgtcgatca aaggctttaa aaaagcaatg agcgatgatg
    19021 aaccaaagca ggataaaacc agtcaggatg ctgattttac tgcgaaaact atcgccgata
    19081 agcaggcgga tacgaatcag gaacaggcta aaacagaaga cgcgaagcgc cacgataaag
    19141 agcaggtgta atccgtgttt gatatcggtt ttagcgaact gctattggtg ttcatcatcg
    19201 gcctcgtcgt tctggggccg caacgactgc ctgtggcggt aaaaacggta gcgggctgga
    19261 ttcgcgcgtt gcgttcactg gcgacaacgg tgcagaacga actgacccag gagttaaaac
    19321 tccaggagtt tcaggacagt ctgaaaaagg ttgaaaaggc gagcctcact aacctgacgc
    19381 ccgaactgaa agcgtcgatg gatgaactac gccaggccgc ggagtcgatg aagcgttcct
    19441 acgttgcaaa cgatcctgaa aaggcgagcg atgaagcgca caccatccat aacccggtgg
    19501 tgaaagataa tgaagctgcg catgagggcg taacgcctgc cgctgcacaa acgcaggcca
    19561 gttcgccgga acagaagcca gaaaccacgc cagagccggt ggtaaaacct gctgcggacg
    19621 ctgaaccgaa aaccgctgca ccttcccctt cgtcgagtga taaaccgtaa acatgtctgt
    19681 agaagatact caaccgctta tcacgcatct gattgagctg cgtaagcgtc tgctgaactg
    19741 cattatcgcg gtgatcgtga tattcctgtg tctggtctat ttcgccaatg acatctatca
    19801 cctggtatcc gcgccattga tcaagcagtt gccgcaaggt tcaacgatga tcgccaccga
    19861 cgtggcctcg ccgttcttta cgccgatcaa gctgaccttt atggtgtcgc tgattctgtc
    19921 agcgccggtg attctctatc aggtgtgggc atttatcgcc ccagcgctgt ataagcatga
    19981 acgtcgcctg gtggtgccgc tgctggtttc cagctcgctg ctgttttata tcggcatggc
    20041 gttcgcctac tttgtggtct ttccgctggc atttggcttc cttgccaata ccgcgccgga
    20101 aggggtacaa gtatccaccg acatcgccag ctatttaagc ttcgttatgg cgttgtttat
    20161 ggcgtttggt gtctcctttg aagtgcctgt ggcaattgtg ctgctgtgct ggatggggat
    20221 tacctcgcca gaagacttac gcaaaaaacg cccgtatgtg ctggttggtg cattcgttgt
    20281 cgggatgctg ctgacaccgc cggatgtctt ctcgcaaacg ctgttggcga tcccgatgta
    20341 ctgtctgttt gaaatcggtg tcttcttctc acgcttttac gttggtaaag ggcgaaatcg
    20401 ggaagaggaa aacgacgctg aagcagaaag cgaaaaaact gaagaataaa ttcaaccgcc
    20461 cgtcagggcg gttgtcatat ggagtacagg atgtttgata tcggcgttaa tttgaccagt
    20521 tcgcaatttg cgaaagaccg tgatgatgtt gtagcgcgcg cttttgacgc gggagttaat
    20581 gggctactca tcaccggcac taacctgcgt gaaagccagc aggcgcaaaa gctggcgcgt
    20641 cagtattcgt cctgttggtc aacggcgggc gtacatcctc acgacagcag ccagtggcaa
    20701 gctgcgactg aagaagcgat tattgagctg gccgcgcagc cagaagtggt ggcgattggt
    20761 gaatgtggtc tcgactttaa ccgcaacttt tcgacgccgg aagagcagga acgcgctttt
    20821 gttgcccagc tacgcattgc cgcagattta aacatgccgg tatttatgca ctgtcgcgat
    20881 gcccacgagc ggtttatgac attgctggag ccgtggctgg ataaactgcc tggtgcggtt
    20941 cttcattgct ttaccggcac acgcgaagag atgcaggcgt gcgtggcgcg tggaatttat
    21001 atcggcatta ccggttgggt ttgcgatgaa cgacgcgggc tggagctgcg ggaattgttg
    21061 ccgttgattc cggcggagaa attgctgatc gaaactgatg cgccgtatct gctcccgcgc
    21121 gatctcacgc caaagccatc atcccggcgc aacgagccag cccatctgcc ccatattttg
    21181 caacgtattg cgcactggcg tggagaagat gccgcatggc tggctgccac cacggatgcc
    21241 aatgtcaaaa cactgtttgg gattgcgttt tagagtttgc ggaactcggt gttcttcaca
    21301 ctgtgcttaa tctctttatt aataagatta agcaatagca tggagcgagc ctcaccatcg
    21361 ggttcggtga aaatggcctg aaagccttcg aacgcgcctt cggtaataat caccttatca
    21421 cccggataag gggttgccgg atcgacaatg tctttcggtt tatataccga tagctgatga
    21481 atcaccgccg atgggactat cgctggcgac gcgccaaagc gcacgaagtg gctgacaccg
    21541 cgggtcgcgt tgatagtcgt ggtatgaatc acttctggat caaattcgac aaacaggtag
    21601 ttgggaaaca atggctcact gactgcagta cgttttccac gcacgatttt ttccagggtg
    21661 atcatcggtg ccaggcaatt cacagcctgt ctttcgaggt gttcctgggc acgttgaagt
    21721 tgcccgcgct tgcagtacag taaataccag gattgcataa tgactcttat ccgtttaatc
    21781 ggggcgcaag gatagcaaaa gctttacgct aagttaatta tattccccgg tttgcgttat
    21841 accgtcagag ttcacgctaa tttaacaaat ttacagcatc gcaaagatga acgccgtata
    21901 atgggcgtag attaagaggc tacaatggac gccatgaaat ataacgattt acgcgacttc
    21961 ttgacgctgc ttgaacagca gggtgagcta aaacgtatca cgctcccggt ggatccgcat
    22021 ctggaaatca ctgaaattgc tgaccgcact ttgcgtgccg gtgggcctgc gctgttgttc
    22081 gaaaacccta aaggctactc aatgccggtg ctgtgcaacc tgttcggtac gccaaagcgc
    22141 gtggcgatgg gcatggggca ggaagatgtt tcggcgctgc gtgaagttgg taaattattg
    22201 gcgtttctga aagagccgga gccgccaaaa ggtttccgcg acctgtttga taaactgccg
    22261 cagtttaagc aagtattgaa catgccgaca aagcggctgc gtggtgcgcc ctgccaacaa
    22321 aaaatcgtct ctggcgatga cgtcgatctc aatcgcattc ccattatgac ctgctggccg
    22381 gaagatgccg cgccgctgat tacctggggg ctgacagtga cgcgcggccc gcataaagag
    22441 cggcagaatc tgggcattta tcgccagcag ctgattggta aaaacaaact gattatgcgc
    22501 tggctgtcgc atcgcggcgg cgcgctggat tatcaggagt ggtgtgcggc gcatccgggc
    22561 gaacgtttcc cggtttctgt ggcgctgggt gccgatcccg ccacgattct cggtgcagtc
    22621 acccccgttc cggatacact ttcagagtat gcgtttgccg gattgctgcg cggtaccaag
    22681 accgaagtgg tgaagtgtat ctccaatgac cttgaagtgc ccgccagtgc ggagattgtg
    22741 ctggaagggt atatcgaaca aggtgaaacg gcgccggaag ggccgtatgg cgaccacacc
    22801 ggttactata acgaagtcga tagtttcccg gtatttaccg tgacgcatat tacccagcgt
    22861 gaagatgcga tttaccactc cacctatact ggtcgtccgc cggatgaacc cgcggtgctg
    22921 ggtgtcgcac tgaacgaagt gtttgtgccg attctgcaaa aacagttccc ggaaattgtc
    22981 gatttttacc tgccgccgga aggctgttct tatcgtctgg cggtagtgac gatcaaaaaa
    23041 cagtacgccg gacacgcgaa gcgcgtcatg atgggcgtct ggtcgttctt acgccagttt
    23101 atgtacacta aatttgtgat cgtttgcgat gatgacgtca acgcacgcga ctggaacgat
    23161 gtgatttggg cgattaccac ccgtatggac ccggcgcggg atactgttct ggtagaaaat
    23221 acgcctattg attatctgga ttttgcctcg cctgtctccg ggctgggttc aaaaatgggg
    23281 ctggatgcca cgaataaatg gccgggggaa acccagcgtg aatggggacg tcccatcaaa
    23341 aaagatccag atgttgtcgc gcatattgac gccatctggg atgaactggc tatttttaac
    23401 aacggtaaaa gcgcctgatg cgcgtttgtt ttgccctatt tatcgatccg acagagaaag
    23461 cgcatgacaa ccttaagctg taaagtgacc tcggtagaag ctatcacgga taccgtatat
    23521 cgtgtccgca tcgtgccaga cgcggccttt tcttttcgtg ctggtcagta tttgatggta
    23581 gtgatggatg agcgcgacaa acgtccgttc tcaatggcct cgacgccgga tgaaaaaggg
    23641 tttatcgagc tgcatattgg cgcttctgaa atcaaccttt acgcgaaagc agtcatggac
    23701 cgcatcctca aagatcatca aatcgtggtc gatattcccc acggagaagc gtggctgcgc
    23761 gatgatgaag agcgtccgat gattttgatt gcgggcggca ccgggttctc ttatgcccgc
    23821 tcgattttgc tgacagcgtt ggcgcgtaac ccaaaccgtg atatcaccat ttactggggc
    23881 gggcgtgaag agcagcatct gtatgatctc tgcgagcttg aggcgctttc gttgaagcat
    23941 cctggtctgc aagtggtgcc ggtggttgaa caaccggaag cgggctggcg tgggcgtact
    24001 ggcaccgtgt taacggcggt attgcaggat cacggtacgc tggcagagca tgatatctat
    24061 attgccggac gttttgagat ggcgaaaatt gcccgcgatc tgttttgcag tgagcgtaat
    24121 gcgcgggaag atcgcctgtt tggcgatgcg tttgcattta tctgagatat aaaaaaaccc
    24181 gcccctgaca ggcgggaaga acggcaacta aaactttatt cagtggcatt tagatctatg
    24241 tcgcatccgg cattcggcac acgatgcctg atgcgacgct ggcgcgtctt atcaggccta
    24301 cattggtgcc ggatcggtag accggataag gcgttcacgc cgcatccggc aagtggttaa
    24361 acccgctcaa acaccgtcgc aataccctga cccagaccga tacacatcgt cgccagacca
    24421 aactggacgt ctttgcgctc catcagattt agcagcgtgg tgctgatacg cgcaccggaa
    24481 cagcccagcg gatgacccag cgcgatcgcg ccaccgttga ggttgatctt ctcgtcaatc
    24541 tgctccatta gccccagatc tttaatacat ggcaggatct gcgcggcaaa ggcttcattc
    24601 atttcaaaca cgccgatatc actgacagaa agccccgctt ttttcagcgc cagcttcgag
    24661 gccggaaccg ggccgtaacc cataatcgat gggtcacaac caacgaccgc catcgaacgc
    24721 acgcgggcgc gcggcttaag acctaattca tgggcgcggc tttcactcat caccagcatg
    24781 gcagctgcgc catcggaaag cgcagaagat gtacccgccg ttaccgtacc gcttactgga
    24841 tcaaacgccg gacgcagcgt ggctagggct tccacggtgg tttccgggcg aatcacttcg
    24901 tcgtaattaa actgcttcag gacgccgtcg gcatcgtgac caccggtcgg gataatttca
    24961 tttttaaatg cacccgactg cgtggcggcc caggcgcggg cgtgtgaccg cgcggcaaag
    25021 gcatcctgca tttcacggct gataccgtgc atacgcgcca gcatttctgc cgttaagccc
    25081 atcatgcccg ccgctttggc gacattgcgg ctcaggccgg ggtgaaaatc aacgccgtga
    25141 ctcatcggca catggcccat atgctccacg ccgccaacca gacatgcctg cgcatcgccg
    25201 gtcataatca tccgtgctgc gtcatgcagt gcctgcatgg atgaaccaca caagcgattg
    25261 acggtaaccg ccgggacaga gtgtggtact tctgccagca gcgccgcgtt acgggcgatg
    25321 ttaaaaccct gctccagcgt ctgctgcaca caaccccagt aaatatcgtc gagggccgcc
    25381 gcttccagcg ccgggttacg cgccagcagg ctacgcatta aatgagcgga gagatcttct
    25441 gcacgcacgt tacgaaaagc accgcccttc gaacggccca tcggggtgcg aattgcatcg
    25501 acaatgacaa cctgttccat tgtgactcct taagccgttt tcaggtcgcc aaccggacgg
    25561 gctggctcaa ccggaggata gtacggttcg ttatgacgcg ctttattacg cagaccttcc
    25621 ggcacttcat acagcgggcc gaggtgctga tattgctgtg ccatatcgag gtattttgcg
    25681 ctaccgaggg tgtccagcca gcggaacgcg ccgccgtgga acggagggaa gcccaggccg
    25741 tagaccagcg ccatatccgc ttccgccgga gtggcgataa tgctttcttc cagacagcgc
    25801 accacttcgt tgaccatcgg gatcatcatg cgggcgataa tctcctcttc gctgaaatcg
    25861 cgcttcggct ggctcacttc tgccagcagg tcgtcaacga cggcgtcttc ttctttcttc
    25921 ggcttacctt tgctgtcttc tttataacgc cagaaaccga ggccgttctt ctgaccaaag
    25981 cggttggcat caaacagcgc gtcgatggta tcgcgataat ctttctgcat ccgctgcggg
    26041 aagcctgctg ccatgacagc ctgggcatga tgcgcggtgt caatgcccac gacatccagc
    26101 agatatgccg gacccatcgg ccagccaaac tgtttttcca ttactttgtc gatcttgcgg
    26161 aaatccgcgc cgtcgcgcag cagctggctg aaaccggcga aatacgggaa cagcacgcgg
    26221 ttgacgaaga agccggggca gtcgttaacc acaatcggcg ttttgcccat cttgctcgcc
    26281 caggcgacga ctttcgcgat ggtttcgtcg gagcttttct cgccgcgaat tatttctacc
    26341 aacggcattc ggtggaccgg gttaaagaag tgcatcccgc agaagttttc tggacgttcc
    26401 agcgcgttgg ccagttcgct gataggaatg gttgaagtgt tagacgccag cacggtattc
    26461 gggcgtactt tctgctcggt ttctgccagt acggcttttt tcacttttgg gttttcgaca
    26521 accgcttcta ccacaacatc cacgcggtca aacccggcgt agtcgagcgt tgggtggatt
    26581 gtggagatca cgccagccag tttcagacca tcgatcttgc cgcgctcaag ctgcttgttc
    26641 agcagtttcg cggcttcggt catgccgagg gttaacgact tgtcgttgat atctttcatg
    26701 acaaccggca cgcctttcca cgcagactgg taagcgatgc cgccgcccat aatgcctgca
    26761 cccagcaccg cggcctgttt cggggtttca acgtctttgg tgagtttctt cgctttgcct
    26821 tttacatatt gatcgttaag gaaaatgccg accagtgcgc gggcttcgtt ggtatgcgcc
    26881 agcgggacaa aacttttgtt ttccaggttt aaggcttctt cacgaccaaa acgggccgca
    26941 gcttcaatgg tttttactgc ggtgatgggg gccggataat gtttccccgc tgtttgtgcg
    27001 accatccctt tagcgatggt gaagctcatg gtggcttcaa tcttgctcag ttttagtggt
    27061 tccagcttcg gctgacgttt tgctttccag tcgaggtcgc cgttaatggc ctggcgtaaa
    27121 accgcctttg cgccttcaac cagtttttct gctttgacta cgccatccac cagaccgatt
    27181 ttcagcgcct gatccgcgcc gacatcttta ccggcggcaa tgatttccag cgcactgtca
    27241 gcgcccagca tacgtggcat acgtacagaa ccgccaaagc ccggcatgat gcccagtttg
    27301 gtttccggca gaccgatgcg cagatccggc gtcgccagac gataatcggt cgccagcacg
    27361 cattcgcagc caccgcccag cgcatagccg ttgacggcag caatggtcgg caccggcaga
    27421 tcttccaggc gattaaacac gctattggca aagtgcagcc actgacttaa ctgttcttca
    27481 ggaacgagga acagggacaa aaattcggtg atatcagcac cgacgataaa ggctgctttg
    27541 ttcgaacgca gcagcagccc ttttagatct gattgctgtt ccagcacgcc gatggcctcg
    27601 ccgaggctgg cgacggtcgc agtgtcgagt ttattaactg aacctggggc atcaaatacc
    27661 agttcggcaa tgccatcttc cagccagtca aggtacaggg tgtcgccttt gtaaagcatg
    27721 tcagtctcct gaatccgcaa ggtgatctgg tcgtaccaga tgcatcgaag tgtgtatttt
    27781 gtgttaaaaa tatgcaaata aaagattaaa gaaatgccga tctgatcacg ctcggcagaa
    27841 atcacgctct ggatgaacga tgtgctaaga tgcggagact taaggtcaaa aaaacagaag
    27901 ggtaaaaaat ggaatcactg gcctcactct ataaaaatca tatagctacc ttacaggaac
    27961 ggactcgcga tgcgctggcg cgcttcaagc tggatgcgtt acttattcac tccggcgaac
    28021 tgttcaacgt ttttctcgac gatcatccct atccgtttaa agtgaacccg caattcaaag
    28081 cgtgggtgcc ggtaactcag gtgccaaact gctggctgct ggtggatggc gtgaacaagc
    28141 cgaaactgtg gttctatctg ccggttgatt actggcacaa cgtcgaaccg ctgccgacct
    28201 ctttctggac tgaagacgtg gaagtgatcg cgctgccgaa agccgatggc attggtagcc
    28261 tgctgcctgc tgcgcgcggg aatatcggtt atatcggtcc ggtgccggaa cgtgcgctgc
    28321 aactgggtat tgaggccagc aatatcaacc cgaaaggggt gatcgactac ctgcattact
    28381 accgctcctt caaaaccgag tacgaactgg cctgtatgcg tgaagcgcag aaaatggcgg
    28441 tcaacggtca tcgcgcggca gaagaagcgt tccgttctgg catgagcgag ttcgatatca
    28501 acattgccta tctgaccgcg accggtcatc gtgataccga cgtaccttac agcaacattg
    28561 tggcgctcaa cgaacacgct gcggtgttgc attacaccaa actggaccac caggcaccgg
    28621 aagagatgcg cagcttcctg ctggatgccg gggccgaata taacggctat gcggctgacc
    28681 tgacccgtac ctggtcggca aaaagtgaca acgactacgc acagctggtg aaagacgtaa
    28741 atgatgaaca actggcgctg atcgctacca tgaaagcagg cgtcagctat gtggattacc
    28801 acatccagtt ccatcagcgc atcgccaaac tgctgcgtaa acatcaaatc atcaccgata
    28861 tgagtgaaga agcgatggtc gaaaacgatc tcaccggacc gtttatgccg cacggtattg
    28921 gccatccgct gggcctgcag gtgcatgacg tagccggttt tatgcaggat gatagcggta
    28981 cgcacctcgc ggcaccggca aaatatccgt acctgcgctg cacccgtatt ctccagccgg
    29041 gcatggtgtt aaccatcgaa ccgggtatct acttcattga atcgctgctg gcaccgtggc
    29101 gtgaagggca gttcagcaag cacttcaact ggcagaaaat tgaagcactg aaaccgttcg
    29161 gcggcattcg tatcgaagac aacgtggtga tccacgaaaa caacgtggaa aacatgaccc
    29221 gggatctgaa actggcgtga tggaaagctg gttaattcct gcggcaccgg tcacggtcgt
    29281 tgaagagatc aaaaaaagcc gcttcataac gatgttggcg cataccgatg gcgttgaggc
    29341 ggcgaaagcg tttgttgaat cggtgcgggc agaacacccc gatgcccgcc accattgcgt
    29401 ggcgtgggtg gcgggtgcgc cggatgattc tcaacagctg ggtttctctg acgacgggga
    29461 gccggcggga acggcaggta aaccgatgct cgcccagcta atgggcagcg gcgtcgggga
    29521 aattaccgct gtggtagtgc gctactacgg cggcatattg cttggcaccg gtgggttagt
    29581 gaaagcgtat ggcggcggcg tgaatcaggc gctgcgccag ctaacgaccc aacgcaagac
    29641 gccattaacc gaatatactt tgcaatgtga atatagtcag ttaaccggca ttgaagcgtt
    29701 gctggggcag tgtgacggca aaattatcaa cagtgattat caggcattcg ttctgctgcg
    29761 ggtggcgctt ccggcggcga aagtggctga attttccgca aagctggcgg attttagccg
    29821 tggttcattg caattgttag cgattgaaga ataatcccca cttcgttttg cagaactaag
    29881 gaagcggcag agatgcattt tcgcgccatt acccgaatcg ttggactact ggtcatctta
    29941 ttttcaggga ccatgattat ccctgggctg gtagcactca tctaccggga tggagcgggc
    30001 cgcgctttta cccagacctt ttttgtcgcc ctcgccattg gctctatgct gtggtggccg
    30061 aaccgcaaag agaagggcga actgaaatcc cgtgaggggt ttctgatagt ggtgctgttt
    30121 tggaccgtgc tgggtagcgt cggtgcgctc ccttttatct tctcggaaag cccgaacctc
    30181 acgattaccg atgcgttttt tgaatctttc tctggcctga ccaccaccgg ggccactacg
    30241 ctggtggggc tggattcgct ccctcatgcc atcctctttt atcgccagat gctgcaatgg
    30301 tttggcggga tggggatcat cgtgttagcg gttgcgatac tgcctatcct cggcgtgggt
    30361 gggatgcagc tctatcgcgc agaaatgccc ggcccgctga aagataacaa aatgcgcccg
    30421 cgaattgcgg aaacggcgaa aaccctgtgg ttgatttatg tcttgctgac cgtcgcctgt
    30481 gcgctggcgt tgtggtttgc cggaatggat gcctttgatg ccatcggcca tagctttgcg
    30541 actatcgcta ttggcggctt ctcgacacat gatgccagta tcggttattt cgacagcccg
    30601 actattaaca ctatcattgc tatcttcctg ctgatctccg gctgtaacta cggtctgcac
    30661 ttttcactgt taagtgggcg tagtctgaag gtttattggc gcgatccgga atttcgcatg
    30721 tttatcggcg tacagtttac gctggtggtt atttgtacac tcgtactgtg gtttcataat
    30781 gtctacagtt cggcgctgat gacaattaac caggcgtttt tccaggtggt atcgatggcg
    30841 acaaccgccg ggtttacgac tgacagcatt gcccgctggc cgctcttttt gccggtactg
    30901 cttttatgtt cagcttttat cggcggttgc gccgggtcaa cgggcggtgg cctgaaagtg
    30961 atccgcatcc tgctgctgtt taagcagggg aaccgtgagc tgaaacgact ggtgcatccg
    31021 aacgccgtct atagcattaa gctggggaat cgcgcactgc cggaacgtat cctcgaagcc
    31081 gtttggggat ttttctccgc ctatgcattg gtgtttattg tcagtatgct ggcgattatc
    31141 gccacgggcg tggatgactt ttctgccttt gcctcagttg ttgcgacatt gaataacctg
    31201 gggccagggc ttggcgtggt tgctgataac tttaccagta tgaacccggt ggctaaatgg
    31261 atcctgattg ccaacatgct gtttggtcgt ctcgaggtct ttacattgct ggtgctcttt
    31321 accccgactt tctggcgtga atgatggagt aatacgtgaa aacattaatt cttttctcaa
    31381 caagggacgg acaaacgcgc gagattgcct cctacctggc ttcggaactg aaagaactgg
    31441 ggatccagac ggatgtcgcc aatgtgcacc gcattgaaga accacaatgg gaaaattatg
    31501 accgtgtggt cattggtgct tctattcgct atggtcacta ccactctgcg ttccaggaat
    31561 ttgtcaaaaa acatgcgacg cgactgaatt cgatgccgag cgccttttat tccgtaaacc
    31621 tggtggcgcg caaaccggag aagcgtactc cacagaccaa cagctacgcg cggaagtttc
    31681 tgatgaactc gcaatggcgt cccgatcgct gcgcggtcat tgccggggcg ctgcgttacc
    31741 cacgttatcg ctggtacgac cgttttatga tcaagctgat tatgaagatg tcaggcggtg
    31801 aaacggatac gcgcaaagaa gttgtctata ccgattggga gcaggtggcg aatttcgccc
    31861 gagaaatcgc ccatttaacc gacaaaccga cgctgaaata agcacaaaga a
//
LOCUS       DAPHDW010000043        31052 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.043, whole genome shotgun sequence.
ACCESSION   DAPHDW010000043 DAPHDW010000000
VERSION     DAPHDW010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 31052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.043"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..264
                     /gene="tuf"
                     /locus_tag="RH057_001954"
     CDS             <1..264
                     /gene="tuf"
                     /locus_tag="RH057_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312930.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3128323.1"
                     /translation="EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMV
                     MPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLS"
     gene            494..877
                     /gene="secE"
                     /locus_tag="RH057_001955"
     CDS             494..877
                     /gene="secE"
                     /locus_tag="RH057_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HDV3128324.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLVAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATVAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            879..1424
                     /gene="nusG"
                     /locus_tag="RH057_001956"
     CDS             879..1424
                     /gene="nusG"
                     /locus_tag="RH057_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HDV3128325.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1583..2011
                     /gene="rplK"
                     /locus_tag="RH057_001957"
     CDS             1583..2011
                     /gene="rplK"
                     /locus_tag="RH057_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HDV3128326.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            2015..2719
                     /gene="rplA"
                     /locus_tag="RH057_001958"
     CDS             2015..2719
                     /gene="rplA"
                     /locus_tag="RH057_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HDV3128327.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            3132..3629
                     /gene="rplJ"
                     /locus_tag="RH057_001959"
     CDS             3132..3629
                     /gene="rplJ"
                     /locus_tag="RH057_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HDV3128328.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3696..4061
                     /gene="rplL"
                     /locus_tag="RH057_001960"
     CDS             3696..4061
                     /gene="rplL"
                     /locus_tag="RH057_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HDV3128329.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4381..8409
                     /gene="rpoB"
                     /locus_tag="RH057_001961"
     CDS             4381..8409
                     /gene="rpoB"
                     /locus_tag="RH057_001961"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HDV3128330.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8486..12709
                     /gene="rpoC"
                     /locus_tag="RH057_001962"
     CDS             8486..12709
                     /gene="rpoC"
                     /locus_tag="RH057_001962"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HDV3128331.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            12922..13461
                     /locus_tag="RH057_001963"
     CDS             12922..13461
                     /locus_tag="RH057_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-shock protein C"
                     /protein_id="HDV3128332.1"
                     /translation="MKQEVEKWRPFGHPDGDIRDLSFLDAHQAVYVQHHEGKEPLEYR
                     FWVTYSLHCFTKDYEHQTNEEKQSLMYHAPKESRPFCQHRYNLARTHLKRTILALPES
                     NVIHAGYGSYAVIEVDLDGGDKAFYFVAFRAFREKKKLRLHVTSAYPISEKQKGKSVK
                     FFTIAYNLLRNKQLPQPSK"
     gene            complement(13871..15004)
                     /gene="thiH"
                     /locus_tag="RH057_001964"
     CDS             complement(13871..15004)
                     /gene="thiH"
                     /locus_tag="RH057_001964"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HDV3128333.1"
                     /translation="MKTFSDRWRQLDWDDIRLRINGKTAVDVERALNASQFTRDDMMA
                     LLSPAASGYLEQLAQRAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYARHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAALTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(15001..15771)
                     /gene="thiG"
                     /locus_tag="RH057_001965"
     CDS             complement(15001..15771)
                     /gene="thiG"
                     /locus_tag="RH057_001965"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HDV3128334.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVTLLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAEMLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHAAQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEAGLLARQSGPGSRSHFAHATSPLTGFLEASA"
     gene            complement(15773..15973)
                     /gene="thiS"
                     /locus_tag="RH057_001966"
     CDS             complement(15773..15973)
                     /gene="thiS"
                     /locus_tag="RH057_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HDV3128335.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(15957..16712)
                     /gene="thiF"
                     /locus_tag="RH057_001967"
     CDS             complement(15957..16712)
                     /gene="thiF"
                     /locus_tag="RH057_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HDV3128336.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVVLNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(16705..17340)
                     /gene="thiE"
                     /locus_tag="RH057_001968"
     CDS             complement(16705..17340)
                     /gene="thiE"
                     /locus_tag="RH057_001968"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HDV3128337.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(17340..19235)
                     /gene="thiC"
                     /locus_tag="RH057_001969"
     CDS             complement(17340..19235)
                     /gene="thiC"
                     /locus_tag="RH057_001969"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HDV3128338.1"
                     /translation="MSATKLTRREQRAHAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARGDTEELTVRSSDYTKARLADDGLDELRFSGVLTPKRAKAGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGARLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAATQTIEMGMADMSENFRARGGEI
                     YLRKEEA"
     regulatory      complement(19315..19415)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(19467..19943)
                     /gene="rsd"
                     /locus_tag="RH057_001970"
     CDS             complement(19467..19943)
                     /gene="rsd"
                     /locus_tag="RH057_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HDV3128339.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            20038..20811
                     /gene="nudC"
                     /locus_tag="RH057_001971"
     CDS             20038..20811
                     /gene="nudC"
                     /locus_tag="RH057_001971"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HDV3128340.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLVQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSILLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            20851..21915
                     /gene="hemE"
                     /locus_tag="RH057_001972"
     CDS             20851..21915
                     /gene="hemE"
                     /locus_tag="RH057_001972"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HDV3128341.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRRELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            21925..22596
                     /gene="nfi"
                     /locus_tag="RH057_001973"
     CDS             21925..22596
                     /gene="nfi"
                     /locus_tag="RH057_001973"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HDV3128342.1"
                     /translation="MDLASLRAQQIELASSVIREDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLMVDVPTIGVAKKRLCGKFEPLSSEPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            22639..23229
                     /locus_tag="RH057_001974"
     CDS             22639..23229
                     /locus_tag="RH057_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HDV3128343.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMSDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLREAGESNIGIIFQQ"
     gene            23416..23688
                     /gene="hupA"
                     /locus_tag="RH057_001975"
     CDS             23416..23688
                     /gene="hupA"
                     /locus_tag="RH057_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HDV3128344.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            23701..24396
                     /locus_tag="RH057_001976"
     CDS             23701..24396
                     /locus_tag="RH057_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HDV3128345.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRAAQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(24398..24823)
                     /gene="zraP"
                     /locus_tag="RH057_001977"
     CDS             complement(24398..24823)
                     /gene="zraP"
                     /locus_tag="RH057_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HDV3128346.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            25061..26437
                     /gene="zraS"
                     /locus_tag="RH057_001978"
     CDS             25061..26437
                     /gene="zraS"
                     /locus_tag="RH057_001978"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HDV3128347.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMHR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTVSESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            26434..27759
                     /gene="zraR"
                     /locus_tag="RH057_001979"
     CDS             26434..27759
                     /gene="zraR"
                     /locus_tag="RH057_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HDV3128348.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGALD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSSAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(27756..29045)
                     /gene="purD"
                     /locus_tag="RH057_001980"
     CDS             complement(27756..29045)
                     /gene="purD"
                     /locus_tag="RH057_001980"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HDV3128349.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFVAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVETFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALAYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDTGPNTGGMGAYSPAPVVTDDVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLGV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTNGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(29057..30646)
                     /gene="purH"
                     /locus_tag="RH057_001981"
     CDS             complement(29057..30646)
                     /gene="purH"
                     /locus_tag="RH057_001981"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HDV3128350.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7341 a         8037 c         8483 g         7191 t
ORIGIN      
        1 gaagtgtaca ttctgtccaa agatgaaggc ggtcgtcata ctccgttctt caaaggctac
       61 cgtccgcagt tctacttccg tactactgac gtgactggta ccatcgaact gccggaaggc
      121 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
      181 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      241 gttgtagcaa aagttctgag ctaattgccg ataacatttg acgcaatgcg cactaaaagg
      301 gcatcatttg atgccctttt tgcacgcttt cgtaccagaa cctggctcat cagtgatttt
      361 ctttgtcata atcattgctg agacaggctc tgttgagggc gtataatccg aaaagctaat
      421 acgcgtttcg atttggtttg cctcgcgatc gcggggtgaa aatgtttgta gaaaacttct
      481 gacaggttgg tttatgagtg cgaataccga agctcaagga agcgggcgcg gcctggaagc
      541 gatgaagtgg gtcgttgtgg tggcattgct cctggtggcg attgtcggca actaccttta
      601 tcgcgacatt atgctgccgc tgcgtgcgct ggccgtagta attctgattg ctgcagcggg
      661 tggtgtcgcg ctgttaacga caaaaggtaa agctaccgtt gcttttgccc gtgaagcgcg
      721 taccgaagtc cgtaaggtca tttggccgac tcgccaggaa acattgcaca ccacgctgat
      781 tgtggctgcg gttaccgcag taatgtcact gatcctgtgg ggactggatg gtattctggt
      841 tcgcctggta tcctttatca ctggcctgag gttctaagat gtctgaagct cctaaaaagc
      901 gctggtacgt cgttcaggcg ttttccggtt ttgaaggccg cgtagcaacg tcgctgcgtg
      961 agcatatcaa attacacaac atggaagatt tgtttggtga agtcatggta ccaaccgaag
     1021 aagtggttga aatccgtggc ggtcagcgtc gcaaaagcga acgtaaattc ttccctggct
     1081 acgtcctcgt tcagatggtg atgaacgacg cgagctggca cctggtgcgc agcgtaccgc
     1141 gtgtgatggg cttcatcggc ggtacttccg atcgtcctgc gccaatcagc gataaagaag
     1201 tcgatgcgat tatgaaccgc ctgcagcagg ttggtgataa gccgcgtccg aaaacgctgt
     1261 ttgaaccggg tgaaatggtc cgtgttaatg atggtccgtt cgctgacttc aacggtgttg
     1321 ttgaagaagt ggattacgag aaatctcgtc tgaaagtgtc tgtttctatc ttcggtcgtg
     1381 cgaccccggt agagctggac ttcagccagg ttgaaaaagc ctaacccagc gatcaaaaaa
     1441 gcggcgattt aatcgttgca caaggcgtga gattggaata caatttcgcg ccttttgttt
     1501 ttatgggcct tgcccgtaaa acgatttttt atatcacggg gagcctctca gaggcgttat
     1561 tacccaactt gaggaattta taatggctaa gaaagtacaa gcctatgtca agctgcaggt
     1621 tgcagctggt atggctaacc cgagtccgcc agtaggtccg gctctgggtc agcagggcgt
     1681 aaacatcatg gaattctgca aagcgttcaa cgcaaaaact gattccatcg aaaaaggtct
     1741 gccgattccg gtagtaatca ccgtttacgc tgaccgttct ttcactttcg ttaccaagac
     1801 tccgccggca gcagttctgc tgaaaaaagc ggctggtatc aagtctggtt ccggtaagcc
     1861 gaacaaagac aaagtgggta aaatttcccg cgctcagctg caggaaatcg cgcagaccaa
     1921 agctgccgac atgactggtg ccgacattga agcgatgact cgctccatcg aaggtactgc
     1981 acgttccatg ggcctggtag tggaggacta agaaatggct aaactgacca agcgcatgcg
     2041 tgttatccgc gagaaagttg atgcaaccaa acagtacgac atcaacgaag ctatcgcact
     2101 gctgaaagag ctggcgactg ctaaattcgt agaaagcgtg gacgtagctg ttaacctcgg
     2161 catcgacgct cgtaaatctg accagaacgt acgtggtgca actgtactgc cgcacggtac
     2221 tggccgttcc gttcgcgtag ccgtatttac ccaaggtgca aacgctgaag ctgctaaagc
     2281 tgcaggcgca gaactggtag gtatggaaga tctggctgac cagatcaaga aaggcgaaat
     2341 gaactttgac gttgttattg cttctccgga tgcaatgcgc gttgttggcc agctgggcca
     2401 ggttctgggt ccgcgcggcc tgatgccaaa cccgaaagtg ggtactgtaa caccgaacgt
     2461 tgctgaagcg gttaaaaacg ctaaagctgg ccaggttcgt taccgtaacg acaaaaacgg
     2521 catcatccac accaccatcg gtaaagtgga ctttgacgct gacaaactga aagaaaacct
     2581 ggaagctctg ctggttgcgc tgaaaaaagc aaaaccgact caggcgaaag gcgtgtacat
     2641 caagaaagtt agcatctcca ccaccatggg tgcaggtgtt gcagttgacc aggctggcct
     2701 gagcgcttct gtaaactaat gcctttacgt gggcggtgat tttgtctaca atcttacccc
     2761 cacgtataat gcttaatgca gacgtatatc cgagatattc gggttgtggc aaggcggcaa
     2821 ctgagtgagt cgccaggagc atagctaact atgtgactgg tgcgaatgaa ggaagccaac
     2881 gccgccacaa gttgaatagc gacggataga aaagatttgt tcgttggagc ctggcctatc
     2941 caggcctccg tcgaagaccg caggagtttc gcaagaaact taatcccctg cgtagacggt
     3001 gacagaacgc taagattatt cttttatatt ctggcttgtt tctgctcacc gtaattaaga
     3061 cgctctctcc gtttggagga gtgaagtgag ttccagagat tttctctggc aaacatccag
     3121 gagcaaagct aatggcttta aatcttcaag acaaacaagc gattgttgct gaagtcagcg
     3181 aagtagccaa aggcgcgctg tctgcagtag ttgcggattc ccgtggcgta actgtagata
     3241 aaatgactga actgcgtaaa gcaggtcgcg aagctggcgt atacatgcgt gttgttcgta
     3301 acaccctgct gcgccgtgct gttgaaggta ctccgttcga gtgcctgaaa gacgcgtttg
     3361 ttggtccgac cctgattgca tactctatgg aacacccggg cgctgctgct cgtctgttca
     3421 aagagttcgc gaaagcgaat gcaaaatttg aggtcaaagc cgctgccttt gaaggtgagc
     3481 tgatcccggc gtctcagatc gaccgcctgg caactctgcc gacctacgaa gaagcaattg
     3541 cacgcctgat ggcaaccatg aaagaagctt cggctggcaa actggttcgt actctggctg
     3601 ctgtacgcga tgcgaaagaa gctgcttaat cgcagttatc tttttaacgc attcgcttac
     3661 gtataaactt attctgatat tcaggaacaa tttaaatgtc tatcactaaa gatcaaatca
     3721 ttgaagcagt tgcagctatg tctgtaatgg acgttgtaga actgatctct gcaatggaag
     3781 aaaaattcgg tgtttccgct gctgctgctg tagctgtagc tgctggcccg gttgaagctg
     3841 ctgaagaaaa aactgaattc gacgtaattc tgaaagctgc tggcgctaac aaagttgctg
     3901 ttatcaaagc agtacgtggc gcaactggcc tgggtctgaa agaagctaaa gacctggtag
     3961 aatctgcacc ggctgctctg aaagaaggcg tgagcaaaga cgacgcagaa gcactgaaaa
     4021 aagctctgga agaagctggc gctgaagttg aagttaaata agccaaccct tccggttgca
     4081 gcctgagaaa tcaggctgat ggctggtgac tttttagtca ccagcctttt tgcgctgtaa
     4141 ggcgccagta gcgtttcaca ctgtttgact actgctgtgc ctttcaatgc ttgtttctat
     4201 cgacgactta atatactgcg acaggacgtc cgttctgtgt aaatcgcaat gaaatggttt
     4261 aagcgtgata gcaacaggca ttgcggaaag tgttccattt tccggtcaac aaaatagtgt
     4321 tgcacaaact gtccgctcaa tggacagatg ggtcgacttg tcagcgagct gaggaaccct
     4381 atggtttact cctataccga gaaaaaacgt attcgtaagg attttggtaa acgtccacaa
     4441 gttctggatg taccttatct cctttctatc cagcttgact cgtttcagaa atttatcgag
     4501 caagatcctg aagggcagta tggtctggaa gctgctttcc gttccgtatt cccgattcag
     4561 agctacagcg gtaattccga gctgcaatac gtcagctacc gccttggcga accggtgttt
     4621 gacgtccagg aatgtcaaat ccgtggcgtg acctattccg caccgctgcg cgttaaactg
     4681 cgtctggtga tctatgagcg cgaagcgccg gaaggcaccg taaaagacat taaagaacaa
     4741 gaagtctaca tgggcgaaat tccgctcatg acagacaacg gtacctttgt tatcaacggt
     4801 actgagcgtg ttatcgtttc ccagctgcac cgtagtccgg gcgtcttctt tgactccgac
     4861 aaaggtaaaa cccactcttc gggtaaagtg ctgtataacg cgcgtatcat cccttaccgt
     4921 ggttcctggc tggacttcga attcgatccg aaggacaacc tgttcgtacg tatcgaccgt
     4981 cgccgtaaac tgcctgcgac catcattctg cgcgccctga actacaccac agagcagatc
     5041 ctcgacctgt tctttgaaaa agttatcttt gaaatccgtg ataacaagct gcagatggaa
     5101 ctggtgccgg aacgcctgcg tggtgaaacc gcatcttttg acatcgaagc taacggtaaa
     5161 gtgtacgtag aaaaaggccg ccgtatcact gcgcgccaca ttcgccagct ggaaaaagac
     5221 gacgtcaaac tgatcgaagt cccggttgag tacatcgcag gtaaagtggt tgctaaagac
     5281 tatattgatg agtctaccgg cgagctgatc tgcgcagcga acatggagct gagcctggat
     5341 ctgctggcta agctgagcca gtctggtcac aagcgtatcg aaacgctgtt caccaacgat
     5401 ctggatcacg gcccatatat ctctgaaacc ttacgtgtcg acccaactaa cgaccgtctg
     5461 agcgcactgg tagaaatcta ccgcatgatg cgccctggcg agccgccgac tcgtgaagca
     5521 gctgaaagcc tgttcgagaa cctgttcttc tccgaagacc gttatgactt gtctgcggtt
     5581 ggtcgtatga agttcaaccg ttctctgctg cgcgaagaaa tcgaaggttc cggtatcctg
     5641 agcaaagacg acatcattga tgttatgaaa aagctcatcg atatccgtaa cggtaaaggc
     5701 gaagtcgatg atatcgacca cctcggcaac cgtcgtatcc gttccgttgg cgaaatggcg
     5761 gaaaaccagt tccgcgttgg cctggtacgt gtagagcgtg cggtgaaaga gcgtctgtct
     5821 ctgggcgatc tggataccct gatgcctcag gatatgatca acgccaagcc gatttccgca
     5881 gcagtgaaag agttcttcgg ttccagccag ctgtctcagt ttatggacca gaacaacccg
     5941 ctgtctgaga ttacgcacaa acgtcgtatc tccgcactcg gcccaggcgg tctgacccgt
     6001 gaacgtgcag gcttcgaagt tcgagacgta cacccgactc actacggtcg cgtatgtcca
     6061 atcgaaaccc ctgaaggtcc gaacatcggt ctgatcaact ctctgtccgt gtacgcacag
     6121 actaacgaat acggcttcct tgagactccg tatcgtaaag tgaccgacgg tgttgtaact
     6181 gacgaaattc actacctgtc tgctatcgaa gaaggcaact acgttatcgc ccaggcgaac
     6241 tccaacctgg atgaagaagg ccacttcgta gaagacctgg taacttgccg tagcaaaggc
     6301 gaatccagct tgttcagccg cgaccaggtt gactacatgg acgtatccac ccagcaggtg
     6361 gtatccgtcg gtgcgtccct gatcccgttc ctggaacacg atgacgccaa ccgtgcattg
     6421 atgggtgcga acatgcaacg tcaggccgtt ccgactctgc gcgctgataa gccgctggtt
     6481 ggtactggta tggaacgtgc tgttgccgtt gactccggtg taactgcggt agctaaacgt
     6541 ggtggtgtcg ttcagtacgt ggatgcttcc cgtatcgtta tcaaagttaa cgaagacgag
     6601 atgtatccgg gtgaagcagg tatcgacatc tacaacctga ccaaatacac ccgttctaac
     6661 cagaacacct gtatcaacca gatgccgtgt gtgtctctgg gtgaaccggt tgaacgtggc
     6721 gacgtgctgg cagacggtcc gtccaccgac ctcggtgaac tggcgcttgg tcagaacatg
     6781 cgcgtagcgt tcatgccgtg gaatggttac aacttcgaag actccatcct cgtatccgag
     6841 cgtgttgttc aggaagaccg tttcaccacc atccacattc aggaactggc gtgtgtgtcc
     6901 cgtgacacca agctggggcc ggaagagatc accgctgaca tcccgaacgt gggtgaagct
     6961 gcgctctcca aactggatga atccggtatc gtttacattg gtgcggaagt gaccggtggc
     7021 gacattctgg ttggtaaggt aacgccgaaa ggtgaaactc agctgacccc agaagaaaaa
     7081 ctgctgcgtg cgatcttcgg tgagaaagcc tctgacgtta aagactcttc tctgcgcgta
     7141 ccaaacggtg tatccggtac ggttatcgac gttcaggtct ttactcgcga tggcgtagaa
     7201 aaagacaaac gtgcgctgga aatcgaagaa atgcagctca aacaggcgaa gaaagacctg
     7261 tctgaagaac tgcagatcct cgaagcgggt ctgttcagcc gtatccgtgc tgtgctggta
     7321 gccggtggcg ttgaagctga gaagctcgac aaactgccgc gcgatcgctg gctggagctg
     7381 ggcctgacag acgaagagaa acaaaatcag ctggaacagc tggctgagca gtatgacgaa
     7441 ctgaaacacg agttcgagaa gaaactcgaa gcgaaacgcc gcaaaatcac ccagggcgac
     7501 gatctggcac cgggcgtgct gaagattgtt aaggtatatc tggcggttaa acgccgtatc
     7561 cagcctggtg acaagatggc aggtcgtcac ggtaacaagg gtgtaatttc taagatcaac
     7621 ccgatcgaag atatgcctta cgatgaaaac ggtacgccgg tagacatcgt actgaacccg
     7681 ctgggcgtac cgtctcgtat gaacatcggt cagatcctcg aaacccacct gggtatggct
     7741 gcgaaaggta tcggcgacaa gatcaacgcc atgctgaaac agcagcaaga agtcgcgaaa
     7801 ctgcgcgaat tcatccagcg tgcgtacgat ctgggcgctg acgttcgtca gaaagttgac
     7861 ctgagtacct tcagcgatga agaagttatg cgtctggctg aaaacctgcg caaaggtatg
     7921 ccaatcgcaa cgccggtgtt cgacggtgcg aaagaagcag aaattaaaga gctgctgaaa
     7981 cttggcgacc tgccgacttc cggtcagatc cgcctgtacg atggtcgcac tggtgaacag
     8041 ttcgagcgtc cggtaaccgt tggttacatg tacatgctga aactgaacca cctggtcgac
     8101 gacaagatgc acgcgcgttc caccggttct tacagcctgg ttactcagca gccgctgggt
     8161 ggtaaggcac agttcggtgg tcagcgtttc ggggagatgg aagtgtgggc gctggaagca
     8221 tacggcgcag catacaccct gcaggaaatg ctcaccgtta agtctgatga cgtgaacggt
     8281 cgtaccaaga tgtataaaaa catcgtggac ggcaaccatc agatggagcc gggcatgcca
     8341 gaatccttca acgtattgtt gaaagagatt cgttcgctgg gtatcaacat cgaactggaa
     8401 gacgagtaat tctcgctcaa acaggtcact gctgtcgggt taaaacccgg cagcggattg
     8461 tgctaactcc gacgggagca aatccgtgaa agatttatta aagtttctga aagcgcagac
     8521 taaaaccgaa gagtttgatg cgatcaaaat tgctctggct tcgccagaca tgatccgttc
     8581 atggtctttc ggtgaagtta aaaagccgga aaccatcaac taccgtacgt tcaaaccaga
     8641 acgtgacggc cttttctgcg cccgtatctt tgggccggta aaagattacg agtgcctgtg
     8701 cggtaagtac aagcgcctga aacaccgtgg cgtcatctgt gagaagtgcg gcgttgaagt
     8761 gacccagact aaagtacgcc gtgagcgtat gggccacatc gaactggctt ccccgactgc
     8821 gcacatctgg ttcctgaaat cgctgccgtc ccgtatcggt ctgctgctcg atatgccgct
     8881 gcgcgatatc gaacgcgtac tgtactttga atcctatgtg gttatcgaag gcggtatgac
     8941 caacctggaa cgtcagcaga tcctgactga agagcagtat ctggacgcgc tggaagagtt
     9001 cggtgacgaa ttcgacgcga agatgggtgc ggaagcaatc caggctctgc tgaagagcat
     9061 ggatctggag caagagtgcg aacagctgcg tgaagagctg aacgaaacca actccgaaac
     9121 caagcgtaaa aagctgacca agcgtatcaa actgctggaa gcgttcgttc agtctggtaa
     9181 caaaccagag tggatgatcc tgaccgttct gccggtactg ccgccagatc tgcgtccgct
     9241 ggttccgctg gatggtggtc gtttcgcgac ttctgacctg aacgatctgt atcgtcgcgt
     9301 cattaaccgt aacaaccgtc tgaaacgtct gctggatctg gctgcgccgg acatcatcgt
     9361 acgtaacgaa aaacgtatgc tgcaggaagc ggtagacgcc ctgctggata acggtcgtcg
     9421 cggtcgtgcg atcaccggtt ctaacaagcg tcctctgaaa tctttggccg acatgatcaa
     9481 aggtaaacag ggtcgtttcc gtcagaacct gctcggtaag cgtgttgact actccggtcg
     9541 ttctgtaatc accgtaggtc catacctgcg tctgcatcag tgcggtctgc cgaagaaaat
     9601 ggcactggag ctgttcaaac cgttcatcta cggcaagctg gaactgcgtg gtcttgctac
     9661 caccattaaa gctgcgaaga aaatggttga gcgcgaagaa gctgtcgttt gggatatcct
     9721 ggacgaagtt atccgcgaac acccggtact gctgaaccgt gcaccgactc tgcaccgtct
     9781 gggtatccag gcatttgaac cggtactgat cgaaggtaaa gctatccagc tgcacccgct
     9841 ggtttgtgcg gcatataacg ccgacttcga tggtgaccag atggctgttc acgtaccgct
     9901 gacgctggaa gcccagctgg aagcgcgtgc gctgatgatg tctaccaaca acatcctgtc
     9961 cccggcgaac ggcgaaccaa tcatcgttcc gtctcaggac gttgtactgg gtctgtacta
    10021 catgacccgt gactgtgtta acgccaaagg cgaaggcatg gtgctgactg gcccgaaaga
    10081 agcagaacgt ctgtatcgct ctggtctggc ttctctgcat gcgcgcgtta aagtgcgtat
    10141 caccgagtat gaaaaagatg ctaacggtga attagtagcg aaaaccagcc tgaaagacac
    10201 gactgttggc cgtgccattc tgtggatgat tgtaccgaaa ggtctgcctt actccatcgt
    10261 caaccaggcg ctgggtaaaa aagcaatctc caaaatgctg aatacctgct accgcattct
    10321 cggtctgaaa ccgaccgtta tttttgcgga ccagatcatg tacaccggct tcgcctatgc
    10381 agcgcgttct ggtgcatctg ttggtatcga tgacatggtc atcccggaga agaaacacga
    10441 aatcatctcc gaggcagaag cagaagttgc tgaaattcag gagcagttcc agtctggtct
    10501 ggtaactgcg ggcgaacgct acaacaaagt tatcgatatc tgggctgcgg cgaacgatcg
    10561 tgtatccaaa gcgatgatgg ataacctgca aactgaaacc gtgattaacc gtgacggtca
    10621 ggaagagaag caggtttcct tcaacagcat ctacatgatg gccgactccg gtgcgcgtgg
    10681 ttctgcggca cagattcgtc agcttgctgg tatgcgtggt ctgatggcga agccggatgg
    10741 ctccatcatc gaaacgccaa tcaccgcgaa cttccgtgaa ggtctgaacg tactccagta
    10801 cttcatctcc acccacggtg ctcgtaaagg tctggcggat accgcactga aaactgcgaa
    10861 ctccggttac ctgactcgtc gtctggttga cgtggcgcag gacctggtgg ttaccgaaga
    10921 cgattgtggt acccatgaag gtatcatgat gactccggtt atcgagggtg gtgacgttaa
    10981 agagccgctg cgcgatcgcg tactgggtcg tgtaactgct gaagacgttc tgaagccggg
    11041 tactgctgat atcctcgttc cgcgcaacac gctgctgcac gaacagtggt gtgacctgct
    11101 ggaagagaac tctgtcgacg cggtaaaagt acgttctgtt gtatcttgtg acaccgactt
    11161 tggtgtatgt gcgcactgct acggtcgtga cctggcgcgt ggccacatca tcaacaaagg
    11221 tgaagcaatc ggtgttatcg cggcacagtc catcggtgaa ccgggtacac agctgaccat
    11281 gcgtacgttc cacatcggtg gtgcggcatc tcgtgcggct gctgaatcca gcatccaggt
    11341 gaaaaacaaa ggtagcatca agctcagcaa cgtgaagtcg gttgtgaact ccagcggtaa
    11401 actggttatc acttcccgta atactgaact gaaactgatc gacgaattcg gtcgtaccaa
    11461 agaaagctac aaagtacctt acggtgcggt actggcgaaa ggcgatggcg aacaggttgc
    11521 tggcggcgaa accgttgcaa actgggaccc gcacaccatg ccggttatca ccgaagtaag
    11581 cggttttgta cgctttactg acatgatcga cggtcagacc attactcgtc agacagacga
    11641 attgaccggt ctgtcttcgc tggtggttct ggattccgca gaacgtacag caggtggtaa
    11701 agatctgcgt ccggcactga aaatcgttga tgctcagggt aacgacgttc tgatcccagg
    11761 taccgatatg cctgcgcagt acttcctgcc gggtaaagcg attgttcagc tggaagatgg
    11821 cgtacagatc agctctggtg acaccctggc gcgtattccg caggaatccg gcggtaccaa
    11881 ggacatcacc ggtggtctgc cgcgcgttgc ggacctgttc gaagcacgtc gtccgaaaga
    11941 gccggcaatc ctggctgaaa tcagcggtat cgtttccttc ggtaaagaaa ccaaaggtaa
    12001 acgtcgtctg gttatcaccc cggtagacgg tagcgatccg tacgaagaga tgattccgaa
    12061 atggcgtcag ctcaacgtgt tcgaaggtga acgtgtagaa cgtggtgacg taatttccga
    12121 cggtccggaa gcgccgcacg acattctgcg tctgcgtggt gttcatgctg tgactcgtta
    12181 catcgttaac gaagtacagg acgtataccg tctgcagggc gttaagatta acgataaaca
    12241 catcgaagtt atcgttcgtc agatgctgcg taaagctacc atcgttaacg cgggcagctc
    12301 cgacttcctg gaaggcgaac aggttgaata ctctcgcgtc aagatcgcaa accgcgaact
    12361 ggaagcgaac ggcaaagtgg gtgcaactta ctcccgcgat ctgctgggta tcaccaaagc
    12421 gtctctggca accgagtcct tcatctccgc ggcatcgttc caggagacca ctcgcgtgct
    12481 gaccgaagca gccgttgcgg gcaaacgcga cgaactgcgc ggcctgaaag agaacgttat
    12541 cgtgggtcgt ctgatcccgg caggtaccgg ttacgcgtac caccaggatc gtatgcgtcg
    12601 ccgtgctgcg ggtgaagctc cggctgcacc gcaggtgact gcagaagacg catctgccag
    12661 cctggcagaa ctgctgaacg caggtctggg cggttctgat aacgagtaat cgttaatccg
    12721 caaataacgt aaaaacccgc ttcggcgggt ttttttatgg ggggagttta gggaaagagc
    12781 atttgtcaga atatttaagg aatttctgaa tactcataat caatgtagag atgactaata
    12841 tcctgaaact gactgaacta attgagtcaa actcggcaag gattcgatac tattcctgtg
    12901 taactttctt aaggaacgag aatgaaacag gaagtggaaa agtggcgacc ttttggacat
    12961 ccggatggtg atattcgtga tttatcattt cttgatgctc atcaggctgt ctacgttcag
    13021 catcatgagg gcaaagagcc tttagagtat cgcttttggg ttacctactc tcttcactgc
    13081 ttcacaaaag attatgaaca tcagacgaac gaagaaaaac aatcgttaat gtaccacgcg
    13141 cctaaagaat ctcgtccctt ctgccagcac cgttataact tagcgcgcac acacttaaaa
    13201 agaactattt tggcgctgcc agaaagcaac gttattcatg ccgggtatgg tagctatgcc
    13261 gtgattgagg tggacttaga cggaggagat aaggcatttt actttgttgc gttcagggct
    13321 ttcagggaaa agaaaaaact ccgtttgcat gtaactagcg cttatcccat ttctgaaaaa
    13381 cagaaaggta aatcagtgaa atttttcacc attgcctaca acttattgag aaataagcag
    13441 cttcctcagc cctcaaaata acaaaaccca ccttaaggtg ggtttcgcca gagaattatc
    13501 tctggtattc agaacgccat taccggactt tgccttgacc ttgcgataat cgcaggttgc
    13561 gggatgtctg aatttcttca gtctgctgca tcctggaaga tgagaacatg tgttcttatt
    13621 ttcgtctcta tcatagttga gtatttactc tcttacaatc agatctcttt cattgctcaa
    13681 caggcgatgg cttcagactt tgcattacgg aatttttaag aaaggcaggg cgaaacgagg
    13741 aagaagcttt tcgccctgga caacattcct gctgacggca ctaccataaa aaaacagaac
    13801 gctttcaaca accttgcgtt tttcatcttc cgtagcggct cggaataaat ttacgttctt
    13861 acattccgtc tcatagtctt tgcgaggccc gtcccagata gctgtcccag tctttccata
    13921 ccggctgcaa accctgagcg gttaacgcgg cagcaaccgc ttccggtctg cgatcgtcgt
    13981 gcggtgagaa ctgttccaac tcggggtgat tatcggcata gccacctggc tgcgttttcg
    14041 agaaggcgct gacgttattg atcgccagcg gaataacgcg atcgcgaaac cacggtgatt
    14101 cccgcgtgga gagtgacagt tcaatctccg gtgcaagcag tcggaaggcg cagatggttt
    14161 gcactaactg gcgttcatcc ataatcgacg caggctcaat gccgccagta cacgggcgca
    14221 ggcgcggaaa ggagacagag taacggcttt gccagtaatg ctgttgcagc catagcaaat
    14281 gttcggcaac catatagcag tcaacgcgcc agttgtcgga aaggccaatt agcgcgccga
    14341 ggcctatctt atcaatcccc gcacgcccca gccgatccgg cgtttccagc cgccagaaga
    14401 agtcctgttt ttttcctttc agatgatggc gggcataagt cgcctcgtga tatgtctcct
    14461 gataaaccat cacgccatcc agaccaagct gctttaactc ggcgtattcc gtctccgcca
    14521 gcggttgcac ttccatttgt agtgaagaga actgttcacg cagggcgggg agatgacgac
    14581 gaaagtaatc catccccact ttcgcctgat gttcaccagt gactaatagc agatgttcaa
    14641 agcccatctc ccgtatagcg gcgctttccc tggcaatatc cgcttcatcc agcgttttgc
    14701 gcttgatgcg attactcatg gaaaatccgc agtacgtgca gtcgttagcg caaagattgg
    14761 aaagataaag cgggacgtag aaactaactg tgttgccaaa tcgctgacgg gtcagacgct
    14821 gcgcccgttg ggccagttgt tccagatagc cactggcggc tggcgataac agcgccatca
    14881 tatcgtcgcg ggtgaattgc gaggcattta gcgcccgctc tacgtcaaca gccgttttgc
    14941 cgttgatacg caggcggatg tcatcccagt ccagttgtcg ccagcgatcg ctgaaggttt
    15001 tcatgccgat gcctccagaa atccagtcag cgggctggtg gcatgagcaa aatgactgcg
    15061 gctgcccggt ccggactgac gtgccagcag gcctgcttct accgccagac gaaatgcctt
    15121 cgccatgttg acgggatcgt ccgctacggc aatcgccgta ttcactaaca ccgcgtcggc
    15181 ccccatttcc agggcctgcg cggcatggct gggaacgccg atgccagcat cgacaaccac
    15241 cggaactgtg gcctgctgga taataatctc cagcatggca cgggtttcca gtccctgatt
    15301 cgagccaatc ggcgcgccga gcggcatcac cgctgcacag ccgacttctt ccagacgttt
    15361 acacaatacc ggatcggccc cgcagtaagg cagcacgaca aatccctgtt gtaccagcat
    15421 ttcggcggct ttcagagttt cgatgggatc gggcaacagc cagcgggcgt cagggtgaat
    15481 ctctaatttt aaccagtttg tgcctaacgc ttcacgtgcc agatgggcgg cgaaaatggc
    15541 ttcttccgct gttttcgccc cggaagtatt tggcagcagg gtcacacccg ccgcgataag
    15601 cggttcgagg atagcgtcgt tgtgctggcg caagtcgaca cgtttcatcg ccagtgtcac
    15661 cagctggctg ccggaagcgc ggatcgcctc caccatcagt tgtgaagaag cgaatttccc
    15721 tgtgccggta aacagatgtg aatcaaacgt tttgtccgca atacgtaaca tctcaacccc
    15781 ctgcaataac ctgaaaaagc aggatctggt cgccatcctg cacgatatgt tgcgtccact
    15841 gctcacgcgg gacgatttgc tgattaatcg ccagagccgc gcccgcttgt cgttggtcga
    15901 gttgctccag tagttcgtga acagtttgcc cggcggcgca ctgcatcggt tgatcgttaa
    15961 acaggatctg cattgcttcc tccgcatacc gggcaaccac tggcgcggcg caacgccagg
    16021 ctgcgccact ggctagactt accatcgaac agtcggagtt ctcccgcagg cgtctctata
    16081 ccgcttaata acttaatggc ttccagtgcc tgcaaagtgc ccatcacccc gaccaccggg
    16141 ccaaccacgc ccgccgtgcg gcagttgcgt tctggctcct ggttatctgg ccacaggcag
    16201 cggtaacacc cctgctccca gggcggcgtc agtaccatca actgaccgcc aaatccgacc
    16261 gcgctggcgg tgataagcgg cgtgttgagt accacgcagg cggcattaat ctcctggcga
    16321 gtcgccatat tgtcggtaca gtcgagcacc acatcggccc gtgcaaccgc atcttttaac
    16381 gcctcacccg ttaaccgttg ttgtaatgct gtcagttgaa tgtcgggatt caactgtgtc
    16441 agtcgctgtt ggctgacctg cgatttcggg cgatcgatat cttcagtggt aaagaggatt
    16501 tgtcgttgca gattgcttaa atgcacatcg tcgtcatctg ccagtaccag cgtcccgacg
    16561 ccagcgcccg ccaggtatag cgcagcaggt gtacccagcc cgccaagacc gataatcagc
    16621 acctggctgt cgagcagttt ttgctgcccg tcaagagcga tatcgtcgag caggatttgg
    16681 cggctataac gcataaaatc acggtcattc atcgccaact cctgcaattt ccagcaactg
    16741 tgccgttgcc aaacgccagt ctgcggcttg agtaatggcg ctgacgacgg cgatactgcc
    16801 gacacccgtt gctatcaccg caggcgcgcg tgccagactg ataccgccaa tcgccacggt
    16861 gggataatcc gccagtcgct caacatgccg tgccagctgt tccagcccct gcggtgcaga
    16921 aggcatctgt ttggtttgcg tcgggaacac atgtcccagc gcgatataag aggggcgtgc
    16981 tgccagcgcg acgtcgattt ccatatcgtc atgtgtcgaa acacccagcc gcaggcctgc
    17041 cgcgcggatg gcattgagat cggtggcttg caaatcttcc tgccccaaat ggacgccata
    17101 cgcctgatgc ttgatcgcga gccgccagta atcgttgata aacaatcgcg cgttatagcg
    17161 gcggcccagc gcaattgccg ccacgacatc ggcttccacc tcttcatcgc gccgatcttt
    17221 gatgcgtagc tggagagtac gtacgcctgc atccaacaga cgttcgatcc actgtacgct
    17281 gtccaccact gggtacagtc ctaaacgaaa aggtacagga ggaaaatcag gctgatacat
    17341 cacgcttcct ccttacgcag gtagatttct ccgcctctgg cacggaagtt ctccgacata
    17401 tccgccattc ccatttcaat agtttgcgtg gcggcgtaat cacgcacttc ctggctgatt
    17461 ttcatcgagc agaatttcgg cccacacatg gagcaaaaat gggcgacttt acctgactct
    17521 tgcggcaggg tttcatcgtg ataagcgcgg gcggtaaacg ggtcgagggc cagattaaac
    17581 tggtcttccc agcgaaattc gaagcgggct ttcgacatgg cgttatcgcg aatttgcgcg
    17641 cccggatgcc ctttcgccag gtcagcggcg tgggcggcaa tcttataggt gataagcccc
    17701 tgcttaacat cttctttatt gggcagaccc agatgctctt ttggcgttac gtaacagagc
    17761 atcgcgcagc caaaccagcc aatcatcgcc gcaccaatcc ccgacgtgaa gtggtcatag
    17821 cccggcgcaa tatcggtagt tagcggcccc agagtgtaaa acggcgcttc gtggcagtgc
    17881 tctaactcct cggtcatatt gcggcggatc atctgcatcg gcacgtggcc tgggccttca
    17941 atcatcacct gcacgtcata ttcccaggca attttggtca gttcgcccag cgtatgcagc
    18001 tcggcaaact gcgcttcatc gttggcgtcc tgaatagaac cggggcgcag accgtcgccc
    18061 agcgacagcg aaacgtcata agcggcacag atttcacaaa tttcgcggaa gtgttgatag
    18121 aggaaatttt cctgatgatg ggagaggcac catttcgcca taatcgaacc gccgcgagag
    18181 acgataccgg tcaggcgttt tgcggtcatc ggcacatagc gcagcagtac gcccgcatgg
    18241 atagtgaagt aatccacacc ttgctcggcc tgttccagca gcgtgtcgcg gaacgcttcc
    18301 caggtaagat cttcggcgat cccgttaacc ttctccagcg cctggtagat cggcactgta
    18361 ccgatcggca ccgggctgtt acgcaaaatc cactcgcggg tttcgtgaat atagcgaccg
    18421 gtggagagat ccatcaccgt atccgctccc cagcgcgtgg accataccag cttttccact
    18481 tcttcttcga tggaagaggt gaccgccgag ttgccgatat tggcgttaac ttttaccagg
    18541 aagttacggc caataatcat cggctccgat tccggatgat taatgttggc cgggataatc
    18601 gcacgtccgg cagcaacttc atcacggaca aattccgcag tgatattttc cggcagacgt
    18661 gcgccaaagc tcattcccgg atgctggtgg cgtaaaactt cgctacgtat gcgctcgcgg
    18721 cccatattct cgcggatggc gatgaattcc atttccggcg tgatgatgcc ctggcgggcg
    18781 tagtgcagtt gggtgacacg gcgtcctgct ttggcgcgtt ttggtgttag tacgccgcta
    18841 aaacgcagtt cgtcgaggcc atcatctgcc agccgcgctt tagtgtaatc ggaactgcgc
    18901 acggtaagtt cttcggtatc gccgcgcgca tcgatccacg gctggcgtag ttttgccagc
    18961 ccttgctgca cgttaatggc aatctgcgga tcaccatacg ggccggaggt gtcgtagacc
    19021 ggaatcgctt cgttttcttc gtactgcggc tgttctttgc taccgccaat tagcgtcggg
    19081 ctaagctgga tctcacgcat cggcacgcga acgccggggt gcgtgccagt gatataaata
    19141 cgttttgagt tgggaaaggc ggtgccttcc agggtgtcga taaaatgttg ggcgtgggcg
    19201 cgctgttcgc ggcgggtcag ttttgttgca gacatagctc attccaaaag ttaaggacgt
    19261 ggcttgtcag acgacggatg aagcaagaga cgatcgccgc aaaggcgatg cgatagcaga
    19321 ttaactcttg ttcccttcgc aggtattagc ctgatcaggt tccgcggatc ccgaataaac
    19381 ggtctcagcc agttaaggca ctccgacaag aaattagccc cgcaaatggg gcattgaatg
    19441 taaattacgc gttaacagca cagaactcaa gcaggatgtt tgacgcgggc ggcgtcaagc
    19501 accagcagaa tcagcttatc ttccagcaca aagcgcgctt ccagcgcctc gccgatgtca
    19561 gataaaacct gttgaaactc aaggtaatta tcatgatcga tagcggtttc caggctggaa
    19621 tcgtagtaat ccataatctg ttgagtattg gcttcaagtt gcggccaaat cttcgcggcg
    19681 cgcgcgagtt gcccgttgcc ttccagctta tgaagaatgc gctcataaat actgaaatgc
    19741 ccggcagaca agtaatcgac caggctctga caaaaatcat caagggcttt ttcgtttagc
    19801 ctcatgtacg attctttgcc aggcttaatg ccaaccagat tgtagtaagc cacgagcaga
    19861 tgcttacgta catgtagcca gcgatcaacc agtttgttac ttcctctgac gcgctccgtc
    19921 aggttatcga gctggttaag catgattgac tccgcaagtt tgtattcaaa aactgctcag
    19981 tgagaaatgt aaaaaccatg ttaaacatgc cagtgatgca aaggtagtgc aagagctatg
    20041 gatcgtataa ttgaaaaatt agatcacggc tggtgggtcg tcagccatga acaaaaatta
    20101 tggttgccga agggagaatt gccatatggc gaagcggcaa atttcgatct tgtgggtcag
    20161 cgcgcactac aaatcggcga atggcagggg gaacctgttt ggttagtaca acagcagcgg
    20221 cgtcacgata tggggtcggt acgtcaggtc attgatctcg atgttgggct gtttcaactg
    20281 gccggacgag gcgtacaact ggcggagttt taccgatcgc ataaatactg tggttactgc
    20341 gggcatgaaa tgtatccgag caaaaccgaa tgggcgatgc tgtgcagcca ttgccgtgag
    20401 cgttactacc cgcaaatcgc cccctgcatt attgttgcta tccgtcgcga tgattcgatc
    20461 ctcctcgccc agcatacccg ccatcgtaac ggtgtccata cagtacttgc cggattcgtc
    20521 gaagtgggcg aaaccctcga gcaggcagtc gcgcgggaag tgatggaaga gagcggaatt
    20581 aaagttaaaa acttgcgtta cgtgacttct cagccgtggc cgtttcctca gtctttaatg
    20641 accgcgttta tggcggaata tgacagcggc gacatcgtga tcgacccgaa agaattgctc
    20701 gaggcgaact ggtatcgcta tgacgatttg ccgttactcc cgccgcccgg caccgtagcg
    20761 cgccgtctga tagaagatac ggtggcgatg tgtcgggcag agtatgagtg atgatacact
    20821 gaccgcctga cgcactaagg aacagccaaa atgaccgaac ttaaaaacga tcgttatctg
    20881 cgggcgctgc tgcgccagcc cgttgatgtc actccagtat ggatgatgcg ccaggcgggt
    20941 cgctatctac cggaatataa agccacgcgc gcccaggcgg gcgattttat gtcgctgtgc
    21001 aaaaacgccg agctggcgtg cgaagtgact ttgcaaccgc tgcgtcgcta cccgctggat
    21061 gcggcgatcc tcttttccga tatcctcacc gtgccggacg cgatggggtt agggctctat
    21121 tttgaagccg gagaaggtcc gcgttttacc tcgccagtca cctgcaaagc cgacgtcgat
    21181 aaactgccaa ttccggaccc ggaagatgag ctgggttacg tgatgaacgc ggtgcgtacc
    21241 attcgtcgcg aactgaaagg cgaagtgccg ctgattggtt tttccggcag cccgtggacg
    21301 ctggcaacct acatggtgga aggcggcagc agcaaagcct tcaccgtgat caaaaaaatg
    21361 atgtatgccg atccgcaggc gctgcacgct ctactcgata aactggcgaa aagcgtcact
    21421 ttgtatctga atgcgcagat taaagccggt gctcaggctg tgatgatttt cgacacctgg
    21481 ggcggcgtgc ttaccgggcg cgattatcaa cagttctcgc tttattacat gcataaaatt
    21541 gttgatggtt tactgcgtga aaacgacggt cgccgcgtac cggtcacgct gtttaccaaa
    21601 ggcggcggac agtggctgga agccatggca gaaaccggtt gcgatgcgct gggcctcgac
    21661 tggacaacgg acatcgccga tgcgcgccgc cgtgtgggca ataaagtcgc gttgcagggt
    21721 aatatggatc cgtcgatgct gtacgcgccg cctgcccgca ttgaagaaga agtagcgact
    21781 atacttgcag gtttcggtca cggcgaaggt catgtcttta accttggtca cggcattcat
    21841 caggatgtgc cgccagaaca tgctggcgtg ttcgtggagg cagtgcatcg actgtctgaa
    21901 cagtatcacc gctaaggagt gattatggat ctcgcgtcat tacgcgctca acaaatcgaa
    21961 ctggcttctt ctgtgatccg cgaggatcga ctcgataaag atccaccgga tctgatcgcc
    22021 ggagccgatg tcgggtttga gcagggcgga gaagtgacgc gagcggcgat ggtgctgctg
    22081 aaatatccct cgcttgagct ggtggagtat aaagttgccc gcatcgccac caccatgcct
    22141 tacattccag gttttctttc cttccgcgaa tatcctgcgc tgctggcagc gtgggagatg
    22201 ctgtcgcaaa agccggattt agtgtttgtc gatggtcatg ggatctcgca tcctcgccgt
    22261 cttggcgtcg ccagccattt tggtttgatg gtggatgtgc caacgatcgg cgtggcgaaa
    22321 aaacggctct gcggtaaatt cgaaccgctc tccagcgaac cgggcgcgct ggccccactg
    22381 atggataaag gcgagcagct ggcctgggtc tggcgcagca aagcgcgctg taacccgttg
    22441 tttatcgcta ccggccatcg ggtcagcgtg gacagcgcgc tggcgtgggt acaacgctgc
    22501 atgaaaggct atcgtctgcc ggagccaacg cgctgggcgg acgcggtggc ctcggaacgt
    22561 ccggcgttcg tgcgctatac agcaaatcag ccctaattca ggtaaactgc ggataatttc
    22621 cgtatttgag aactcaacat gttacaaaac ccaattcatc tgcgtctgga gcgcctggaa
    22681 agctggcagc atgtcacttt catggcttgc ttatgcgaac gcatgtaccc caattacgcc
    22741 atgttctgcc agcaaaccgg ttttggtgat gggcaaattt accgtcgtat tctcgatctc
    22801 atctgggaaa cgctgaccgt taaagacgca aaagtaaatt tcgacagcca actggagaaa
    22861 tttgaagaag cgattccttc agccgacgat ttcgatctgt acggcgttta tccggcaatc
    22921 gatgcctgcg tggcgttaag tgaactggtc cattcgcgtt tgagtggtga aacgctcgaa
    22981 cacgcggtgg aagtgagtaa gacctccatc acgaccgttg cgatgctgga aatgactcag
    23041 gctggtcgcg aaatgagcga tgaagagctt aaagaaaacc cagctgtaga gcaagaatgg
    23101 gacattcagt gggaaatatt ccgactttta gctgagtgcg aagaacgcga tatcgagctg
    23161 ataaaaggcc ttagggcaga cctgcgtgag gcgggtgaga gcaatattgg tataattttt
    23221 cagcaataag accagaaaac gtgatttaac gcctgatttg tcgtacctgg agtcttccct
    23281 ttcgcccccc gtctggtcta catttggggg gcgaaaaaaa gtggctatcg gtgcgtgtat
    23341 gcaggagagt gctattctgg catttccgtc gcactcgatg cttagcaagc gataaacaca
    23401 ttgtaaggat aacttatgaa caagactcaa ctgattgatg taattgcaga gaaagcagaa
    23461 ctgtccaaaa cccaggctaa agctgctctg gagtccactc tggctgcaat tactgagtct
    23521 ctgaaagaag gcgatgctgt acaactggtt ggtttcggta ccttcaaagt gaaccaccgc
    23581 gctgagcgta ctggccgcaa cccgcagacc ggtaaagaaa tcaaaattgc cgcagctaac
    23641 gtaccggcat ttgtttctgg caaggcactg aaagacgcag ttaagtaaga ttgcgtggca
    23701 gtgaacagtt ttaacgaagg ggtggtttca ccccttttgt ctttctggcg tcgatcattg
    23761 atgctggcag gtgcgctgtt tctcactgcc tgtagccaca actcttcact tcctcccttt
    23821 accgccagtg gatttgctga agaccagggc gcggtacgca tctggcgaaa agacagcggc
    23881 gataatgtgc atctgcttgc cgtgtttagc ccgtggcgca gtggcgatac cacgacgcga
    23941 gagtatcgct ggcagggcga taacctcacg ctcatcaata tcaatgttta cagcaaaccg
    24001 ccggtgaaca ttcgcgcgcg ttttgacgat cgcggtgatc tgagctttat gcaacgtgaa
    24061 tccgatgggg aaaagcagca gctttctaac gaccaaatcg atttataccg ttatcgtgct
    24121 gctcagatcc gccagattag cgatgcctta cgtcagggga gagtcgtgct gcgccagggg
    24181 cgctggcatg cgatggaaca gaccgtgacc acctgcgaag ggcaaaccat taaacctgat
    24241 ttagattcgc aggcgatagc gcatatcgag cgccgccaga gccgctcttc tgttgatgtc
    24301 agcgtggcat ggctggaagc gcccgaaggt tcgcaattac tgttagtggc aaactctgat
    24361 ttctgtcgct ggcaacccaa cgagaaaacg ttctgattta ccagtggccc atacccatat
    24421 gaccgccacc accgcagccg ccgtagccca tgcccattcc ggctccgcgc ggaatacccg
    24481 cttcagccat cgcgatatcc cgtttcaccc gtaactcatc taacgactga cgcaaattct
    24541 ccatctcttt ggcgaccgcg ttaattttgc tgctatccgg tgggttcgcg gctaacaggg
    24601 cattgtattc ataacgcttc gtcaccagtt gctgttgcag tgcgctgctt tgagcgtaaa
    24661 agtcattatg gattttctgc cacgctgtct gttgttcgct ggtcaaaggc gcggcatttt
    24721 gctgccacat accgtgtccg ccgtgagcaa atgcagatgt cgatcccatc gccattgctg
    24781 aaagcgccat cattaccagg gcaattttcg tgttccgttt catggttaat cctccagtgg
    24841 ttgtcttact tcgggtattg catcttccgt gccaacgatg aaacgctgat atgacgggta
    24901 atctggcatg ataaacgagt aaaaatgact cgcctgctgc gggtagcgag tcatttttac
    24961 tcattgaaac ttaagccttt gtgttacagc gcagggtaag cgctgataaa agatggcatg
    25021 atttctgctg tcagaaaggg atgagcaggc aaagaagaag atgcgtttta tgcaacgttc
    25081 taaagactcc ttagctaaat ggttaagcgc gatcctcccc gtggtcattg ttgggctggt
    25141 agggttgttt gcggtgacgg tgattcgcga ttatgggcgc gagactgccg ccgccagaca
    25201 aacgctgctg gaaaaaggca gtgtacttat tcgcgctctt gaatccggct cgcgcgtcgg
    25261 catggggatg cgcatgcatc atgcgcagca gcaggcctta ctggaagaaa tggccgggca
    25321 gcctggagta cgttggtttg cggtcacgga tgaacaagga acaatcgtga tgcatagcaa
    25381 ctccggcatg gtgggaaaac agctttattc cccgcaggaa atgcagcagt tacatcctgg
    25441 agatgaagaa gcgtggcggc ggatcgatag cgcagacggc gagcctgttc tggaaattta
    25501 tcgccagttt caaccgatgt ttgctgctgg aatgcaccgg atgcgccata tgcagcagta
    25561 tgccgcgaca ccacaagcaa ttttcatcgc tttcgacgcc agtaatattg tgagtgccga
    25621 agatcgtgag cagagaaaca ccctgattat cctcttcgcc ctggcgacgg tcttgctggc
    25681 aagcgtgttg tcattcttct ggtatcgccg ctatctgcgc tcgcgccagc tgttgcagga
    25741 tgaaatgaag cgcaaagaga agctggtagc tctggggcat cttgcggcag gcgttgccca
    25801 cgaaatccgt aatccacttt cctcaattaa agggctggcg aaatactttg ccgaacgcgc
    25861 gccagcaggg ggagaagcgc atcaattggc gcaggtgatg gcgaaagaag ccgaccgttt
    25921 aaaccgcgtg gtaagcgagt tgctggaact ggttaagcca acgcatctgg ctttgcaggc
    25981 ggtggatctc aacacgctga ttaaccactc attacagctg gtaagccagg atgcaaacag
    26041 ccgggagatc cagttacgct ttaccgccaa cgacacatta ccggaaattc aggccgatcc
    26101 ggacaggctg actcaggtcc tgttgaatct ctatctcaat gctattcagg cgattggtca
    26161 gcatggcgtg attagcgtga cggtcagcga aagcggcgcg ggcgtgaaaa tcagcgttac
    26221 cgacagcggt aagggaattg cggcagatca gcttgaagcc atcttcactc cgtacttcac
    26281 caccaaagcc gaaggcaccg gattggggct ggcggtcgtg cataatattg ttgaacaaca
    26341 cggtggtaca attcaggtcg caagccagga gggaaaaggc tcaacgttca ccctctggct
    26401 tccggtcaat attacgcgta aggacccaca aggatgacgc acgataatat cgatattctg
    26461 gtggtggatg atgacattag ccactgcact attttgcagg ctttactgcg cggctggggc
    26521 tataacgtcg cgctggcgaa cagcgggcga caggcgctgg agcaggtgcg ggaacaggtt
    26581 tttgatcttg tgctttgcga tgtgcgaatg gcggagatgg acggcatcgc cacgctgaaa
    26641 gagatcaaag cgttaaaccc ggcaattccg gtgctgatta tgactgcgta ctccagcgtc
    26701 gagacggcgg tagaggcact gaaaactggg gcgctggatt atctcatcaa gccgctggat
    26761 ttcgataacc tacaggcgac gctggaaaag gcgctcgcgc atacgcacag tattgatgct
    26821 gaaacacctg cggtgactgc cagccagttc ggtatggtcg gtaaaagctc ggcgatgcaa
    26881 cacctgctca gtgaaatcgc cctcgtcgcg ccatcggaag ccacggtact gatccacggc
    26941 gattccggca ccggtaaaga gctggtcgcc agggcgattc acgccagtag cgcacgtagc
    27001 gaaaaaccgc tggtaacgct caactgtgcg gcactcaacg aatccttgct ggaatctgaa
    27061 ttgttcggtc acgaaaaagg ggcgtttact ggagccgata aacgccggga agggcgcttt
    27121 gttgaggcgg acggcggcac gctgtttctc gatgaaattg gcgatatctc gccgatgatg
    27181 caggtgcgtc tgctgcgtgc gattcaggag cgcgaagttc agcgtgtcgg tagcaaccag
    27241 actatctcgg ttgatgtccg gctgattgcg gcgacccatc gcgatcttgc cgcagaggtg
    27301 aatgccgggc gttttcgcca ggatctctac tatcgcctga atgtggtggc gattgaagta
    27361 ccatcgctgc ggcaacggcg ggaagatatc cctctgctgg ctggccattt tctgcagcgc
    27421 tttgccgagc gtaatcgcaa ggcggtaaaa ggttttacgc cccaggcgat ggatctgttg
    27481 attcattacg actggccggg aaatattcgt gagctggaaa acgcggtgga acgggcagtg
    27541 gtgctgctga ccggggaata tatttccgaa cgcgagctgc cgctggcgat tgccagtacg
    27601 ccgatcccgc tggggcaaag tcaggatatt cagccgctgg tggaagtgga aaaagaggtg
    27661 attctggcgg cactggagaa aacgggcggc aacaaaactg aagccgcccg tcagttaggg
    27721 atcacgcgta aaacgttatt ggcaaaactg tcgcgttagt tctgctcgcg ttcgatagca
    27781 cgccagccga tatctttccg gcagaagcag tcgtcccagt ggatatcggt cattaaggca
    27841 taggcgcgtt tctgcgcttc tgccacggta tgacccagcg cggtgacgca cagtacgcgt
    27901 ccgccgttgg ttaccacctg ctcgtcatcc gccagttttg tgcccgcgtg gaacactttg
    27961 ccgtcagcca cttcttccag cggcaggccg tggatcacat cgccggtgcg gtaatcgccc
    28021 ggatatccac ccgcagccat caccacgccg agagaagcgc gttcatccca ctcggacgtt
    28081 ttctcgtcca gcttgccttc acaggccgcc aggcagagct caaccagatc ggacttcatg
    28141 cgcagcataa tcggctgggt ttccggatcg ccaaagcggc agttaaattc aataaccttc
    28201 ggattgccct gtttgtcgat catcaggccc gcatagagaa aaccggtgta ggtgttgcct
    28261 tccgccgcca tgcctttcac ggttggccag ataatacgtt ccatggtgcg ctgatgaacg
    28321 tcatcggtta ctaccggcgc gggggagtaa gcgcccatcc cgccggtgtt tggtccggta
    28381 tctttatcgc ctacgcgttt gtgatcctgg ctggtagcca tcggcagcac atgctcgccg
    28441 tccaccatca cgataaagct cgcttcttcg ccatcgagga actcttcgat aacgatgcga
    28501 tgacccgcgt cgccaaaagc gttgcccgcc agcatatcgt gaacagccgc ttccgcttct
    28561 tccagcgtca tcgccacgat aacgcctttc ccggcagcca gaccgtccgc tttaatgacg
    28621 attggcgcgc ctttctcacg cagatacgcc agcgcaggtt ctacctcggt gaagttctgg
    28681 tattccgccg tagggatctt atggcgggcc aggaaatctt tggtaaacgc ttttgagcct
    28741 tccagttggg ccgcacctgc ggttgggccg aagattttca gcccggcggc gcggaaggtc
    28801 tcgaccacgc ctttcaccag cggcgcttcc gggccgacga tggtcagatc aatcttttcg
    28861 ttttgtgcga aatccaacag cgccgggata tcggtcacgc caatagcaac gttttgcagc
    28921 gcgggttcca gcgcagtgcc tgcattaccc ggagcaacaa aaacagtctc aaccagcggc
    28981 gactgggccg ctttccaggc cagcgcgtgc tcgcgcccgc cgttaccaat cactaatact
    29041 ttcatctatt gctccattaa tggcggaagt ggcgcatgtc ggtgaagagc atcgcaatac
    29101 cgtgctcgtc ggcggcggca atcacttcgt catcacggat ggaaccgcca ggctggatta
    29161 cgcaggtcac gcccgcagcg gcggcggcat caataccgtc gcggaacggg aagaacgcgt
    29221 cagaagccat cgaggaacct ttcacttcca ggccttcatc agcagcttta ataccggcga
    29281 ttttcgcgga gtacacgcgg ctcatctggc ccgcgccaat accgatggtc atattgtttt
    29341 tggcatagac gatagcattg gatttcacga acttcgccac tttccagcag aacagcgcat
    29401 cgcgcagttc ctgttcggtc ggctgacgtt gggtgacgac gcgcagttct tccgcaccga
    29461 ccatccccag gtcacgatcc tgaaccagca gaccgccgtt cacgcgtttg aaatcaagac
    29521 ccggaacacg ctcgccccac tgaccgcagg tcagaacgcg tacgttctgc ttggcggcgg
    29581 tgattttcag ggcttcttcg ctggcggaag gcgcaataat cacttcgaca aactgacgag
    29641 aaatgatggc ctgtgcggtt tccgcatcca gctcgcggtt aaaggcaatg atgccgccga
    29701 atgcggaggt tgggtcggtt ttgtacgcgc gatcgtaagc atcaagaatg gaattgccga
    29761 tagctacacc gcaagggttg gcgtgcttca caatcacaca tgccggctcg gcgaactctt
    29821 tcacgcactc cagcgccgca tcggtatcgg cgatgttgtt ataagagagg gctttaccct
    29881 gaacctgggt tgcggtagca acggaggctt ctttcacatt ctcttctata tagaaggcag
    29941 cctgctggtg gctgttctcg ccgtaacgca tatcctgctt cttaatgaag ttcaggttca
    30001 gcgtgcgtgg gaagcgacca gcggcttctt tgctttcacc gtggtacgcc ggaaccatgc
    30061 tgccgaagta gttggcaatc atgctgtcgt aggcggcagt gtgttcgaag gctttgatgg
    30121 cgaggtcgaa acgggttgca agcgtcagcg atccttcgtt gtcatccatc tctttaataa
    30181 tggcgtcgta gtcgctgctc ttcaccacga ttgcgacatc tttatggttc ttagcggcgg
    30241 agcgcaccat ggtcgggccg ccgatatcga tgttctcaac cgcatcttcc agcgagcaac
    30301 cttcacgggc cacggtctgg gcgaacggat acaggttaac aaccaccata tcgataggct
    30361 ggatctgatg ttcttccata atggcatcgt cctggccgcg acggcccaga atgccaccat
    30421 gtactttcgg atgcagggtc ttcacgcgtc catccatcat ctccgggaaa ccggtgtaat
    30481 cggaaacttc ggttaccggc agaccttttt ctgctaacag acgggcagtg ccccctgtag
    30541 acagcagctc cacaccgcgt gcggaaagtg cctgggcgaa ttcgacgata ccggctttgt
    30601 cagaaacact gagcagagcg cggcggactg gacgacgttg ttgcatggta aatcccctgg
    30661 atttgactat tacagagagc gttagctgaa tttttcgcga aaaactcagc taacgcccct
    30721 aacggggcat ccttattttt cgcccgcatt gtaacgaaaa cgtttgcgca acgctcgcga
    30781 atttttctct ttcaatggtg atcacaattt tgacagtggt taccgtggga aaaatacaga
    30841 aattacattg atgattgtgg ataactctgt gcgtaaaaag gtataaagcg gggttttgct
    30901 ggggaatgca gcagtcagtc atttttctgc aatttttcta ttgcggcctg cggagaactc
    30961 cctataatgc gcctccatcg acacggcgga tgtgaatcac ttcacacaaa cagccggttc
    31021 ggttgaagag aaaaatcctg aaattcaggg tt
//
LOCUS       DAPHDW010000044        30163 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.044, whole genome shotgun sequence.
ACCESSION   DAPHDW010000044 DAPHDW010000000
VERSION     DAPHDW010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30163
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.044"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            168..1751
                     /locus_tag="RH057_001982"
     CDS             168..1751
                     /locus_tag="RH057_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HDV3128351.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAETASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEDEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(2025..3878)
                     /gene="asmA"
                     /locus_tag="RH057_001983"
     CDS             complement(2025..3878)
                     /gene="asmA"
                     /locus_tag="RH057_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HDV3128352.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQVQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGQVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVKMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(3900..4481)
                     /gene="dcd"
                     /locus_tag="RH057_001984"
     CDS             complement(3900..4481)
                     /gene="dcd"
                     /locus_tag="RH057_001984"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HDV3128353.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(4573..5214)
                     /gene="udk"
                     /locus_tag="RH057_001985"
     CDS             complement(4573..5214)
                     /gene="udk"
                     /locus_tag="RH057_001985"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HDV3128354.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            5532..8849
                     /locus_tag="RH057_001986"
     CDS             5532..8849
                     /locus_tag="RH057_001986"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV3128355.1"
                     /translation="MSKQSQHVLIALPHPLLHLVSLGLVSFIFTLFSLELSQFGTQLA
                     PLWFPTSIMMVAFYRHAGRMWPGIALSCSLGNIAASILLFSTSSLNMTWTTINIVEAV
                     VGAVLLRKLLPWYNPLQNLADWLRLAFGSAIVPPLLGGVLVVLLTPGDDPLRAFLIWV
                     LSESIGALALVPLGLLFKPHYLLRHRNPRLLFESLLTLAITLTLSWLSMLYLPWPFTF
                     IIVLLMWSAVRLPRMEAFLIFLTTVMMVSLMMAADPSLLATPRTYLMSHMPWLPFLLI
                     LLPANIMTMVMYAFRAERKHISESETRFRNAMEYSAIGMALVGTEGQWLQTNKALCQF
                     LGYSQEELRGLTFQQLTWPEDLNKDLQQVEKLISGEINTYSMEKRYYNRNGDVVWALL
                     AVSLVRHTDGTPLYFIAQIEDINELKRTEQVNQQLMERITLANEAGGIGIWEWELKPN
                     IFSWDKRMFELYEIPPHIKPNWQVWYECVLPEDRQHAEKVIRDSLQSRSPFKLEFRIT
                     VKDGIRHIRALANRVLNKEGEVERLLGINMDMTEVKQLNEALFQEKERLHITLDSIGE
                     AVVCIDMAMKITFMNPVAEKMSGWTQEEALGVPLLTVLHITFGDNGPLMENIYSADTS
                     RSAIEQDVVLHCRSGGSYDVHYSITPLSTLDGSNIGSVLVIQDVTESRKMLRQLSYSA
                     SHDALTHLANRASFEKQLRMLLQTVNSTHQRHALVFIDLDRFKAVNDSAGHAAGDALL
                     RELASLMLSMLRSSDVLARLGGDEFGLLLPDCNVESARFIATRIISAVNDYHFIWEGR
                     VHRVGASAGITLIDDNNHQAAEVMSQADIACYASKNGGRGRVTVYEPQQAAAHSERAA
                     MSLDEQWRMIKENQLMMMAHGVASPRIPEARNLWLISLKLWSCEGEIIDEQKFRRSFS
                     DPALSHALDRRVFHEFFQLAAKAVASKGISIALPLSVAGLSSATLVNDLLEQLENSPL
                     PPRLLHLIIPADAILDHAESVQKLRLAGCRIVLSQVGRDLQIFNSLKANMADYLLLDG
                     ELCANVQGNLMDEMLITIIQGHAQRLGMKTIAGPVVLPLVMDTLSGIGVDLIYGDVIA
                     DAQPLDLLVNSSYFAIN"
     gene            complement(8888..9745)
                     /gene="alkA"
                     /locus_tag="RH057_001987"
     CDS             complement(8888..9745)
                     /gene="alkA"
                     /locus_tag="RH057_001987"
                     /EC_number="3.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2"
                     /protein_id="HDV3128356.1"
                     /translation="MYTLNWQPPYDWSWMLGFLAARAVSGVETVADSYYARSLAVGEY
                     RGVVTAIPDIARHTLHINLSAGLEPVAAECLAKMSRLFDLQCNPQIVNGALGKLGAAR
                     PGLRLPGCIDAFEQGVRAILGQLVSVAMAAKLTAKVVQLYGERLDDFPEYICFPTPQR
                     LAAADPQALKALGMPLKRAEALIHLANAALEGSLPMTIPGDVEQAMKTLQTFPGIGRW
                     TANYFALRGWQAKDVFLPDDYLIKQRFPGMTPAQIRRYAERWKPWRSYALLHIWYTEG
                     WQPDGTDEL"
     gene            9879..11231
                     /gene="yegD"
                     /locus_tag="RH057_001988"
     CDS             9879..11231
                     /gene="yegD"
                     /locus_tag="RH057_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416573.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3128357.1"
                     /translation="MFIGFDYGTANCSVAVMRDGKPQLLKMENDSTLLPSMLCAPTRE
                     SVSEWLYRHHDVPADDDETQALLRRAIRYNREEDIDVTAKSVQFGLSSLAQYIDDPEE
                     VWFVKSPKSFLGASGLKPQQVALFEDLVCAMMLHIRQQAQAQLPEAITQAVIGRPINF
                     QGLGGDEANAQAQGILERAAKRAGFRDVVFQYEPVAAGLDYEATLQEEKRVLVVDIGG
                     GTTDCSLLLMGPQWRSRLDREASLLGHSGCRIGGNDLDIALAFKNLMPLLGMGGETEK
                     GIALPILPWWNAVAINDVPAQSDFYSSANGRLLNDLVRDAREPEKVALLQKVWRQRLS
                     YRLVRSAEECKIALSSVAETRASLPFISDELATLISQQGLESALSQPLARILEQVQLA
                     LDNAQEKPDVIYLTGGSARSPLIKKALAEQLPGIPIAGGDDFGSVTAGLARWAEVVFR
                     "
     gene            complement(11243..13183)
                     /gene="yegI"
                     /locus_tag="RH057_001989"
     CDS             complement(11243..13183)
                     /gene="yegI"
                     /locus_tag="RH057_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YegI"
                     /protein_id="HDV3128358.1"
                     /translation="MKPTLYTATGECVTPGRELGKGGEGAVYDINEFVDSVAKIYHTP
                     PPALKQDKLAFMAATADAQLLNYVAWPQATLHGGRGGKVIGFMMPKVSGKEPIHMIYN
                     PAHRRQRYPHCAWDFLLYVARNIASSFATVHEHGHVVGDVNQNSFMVGRDSKVVLIDS
                     DSFQINANGTLHLCEVGVSHFTPPELQTLSSFVGFERTANHDNFGLALLIFHVLFGGR
                     HPYSGVPLISDAGNALETDITHFRYAYASDNQRRGLKPPPRSIPLSMLPSDVEAMFQQ
                     AFTESGVATGRPTAKAWVAALDSLRQQLKKCPVSAMHVYPAHLTDCPWCALDNQGVIY
                     FIDLGEEVITTGGDFVLAKVWAMVMASVAPPALQLPLPDHFQPTGRPLPLGLLRREYI
                     ILLEIALSALSLLLCGLQAEPRYIILVPVLAAIWIIGSLTSKAYKAEVQQRREAFNRA
                     KMDYDHLVRQIQQVGGLEGFIAKRTMLEKMKDEILGLPEEEKRALAALHDTARERQKQ
                     KFLEGFFIDVASIPGVGPARKAALRSFGIETAADVTRRGVKQVKGFGDHLTQAVIDWK
                     ASCERRFVFRPNEAITPADRQAVMAKMTAKRHRLESTLTVGATELQRFRLHAPARTMP
                     LMEPLRQAAEKLAQAQADLSRC"
     gene            complement(13180..13941)
                     /gene="pphC"
                     /locus_tag="RH057_001990"
     CDS             complement(13180..13941)
                     /gene="pphC"
                     /locus_tag="RH057_001990"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase PphC"
                     /protein_id="HDV3128359.1"
                     /translation="MSWRLVYASAVGTSHISADLPCQDACQMQVAWLNDQQPLLVMFL
                     ADGAGSVSQGGEGAMLAVNEAMAYMSQKVQGGELGLNDILATDIVLTIRQRLFAEAEA
                     KELAVRDFACTFLGLISSANGTLIMQIGDGGVVVDFGHGLQLPLTPMVGEYANMTHFI
                     TDEDAVSRLETFTSTERVHKVAAFTDGIQRLALNMLDNSPHVPFFTPFFNGLASATQE
                     QLDLLPELLKQFLSSPAVNERTDDDKTLALALWLP"
     gene            complement(13938..14597)
                     /locus_tag="RH057_001991"
     CDS             complement(13938..14597)
                     /locus_tag="RH057_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV3128360.1"
                     /translation="MSEQITFATSDFASNPEPRCPCILLLDVSGSMSGRPINELNAGL
                     VAFRDELLADPLALKRVELGIVTFGPVHVEQPFTSAANFFPPILFAQGDTPMGAAITK
                     ALDMVEERKREYRANGISYYRPWIFLITDGAPTDEWQAAANKVFQGEEDKKFAFFSIG
                     VQGADMKTLAQISVRQPLPLQGLQFRELFSWLSSSLRSVSRSTPGTEVVLEAPKGWTS
                     V"
     gene            complement(14818..14877)
                     /gene="ibsA"
                     /locus_tag="RH057_001992"
     CDS             complement(14818..14877)
                     /gene="ibsA"
                     /locus_tag="RH057_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsA"
                     /protein_id="HDV3128361.1"
                     /translation="MMKHVIILVILLVISFQAY"
     gene            complement(15150..15206)
                     /locus_tag="RH057_001993"
     CDS             complement(15150..15206)
                     /locus_tag="RH057_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3128362.1"
                     /translation="MMKLLIIVVLLVISFPAY"
     gene            complement(15478..15534)
                     /locus_tag="RH057_001994"
     CDS             complement(15478..15534)
                     /locus_tag="RH057_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HDV3128363.1"
                     /translation="MMKLLIIVVLLVISYPAY"
     gene            15813..17060
                     /gene="mdtA"
                     /locus_tag="RH057_001995"
     CDS             15813..17060
                     /gene="mdtA"
                     /locus_tag="RH057_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
                     /protein_id="HDV3128364.1"
                     /translation="MKGSYKSRWVIVIVVVIAAIAAFWFWQGRNDSQSAAPGATKQAQ
                     QSPAGGRRGMRSGPLAPVQAATAVEQAVPRYLTGLGTITAANTVTVRSRVDGQLMALH
                     FQEGQQVKAGDLLAEIDPSQFKVALAQAQGQLAKDKATLANARRDLARYQQLAKTNLV
                     SRQELDAQQALVSETEGTIKADEASVASAQLQLDWSRITAPVDGRVGLKQVDVGNQIS
                     SGDTTGIVVITQTHPIDLVFTLPESDIATVVQAQKAGKPLVVEAWDRTNSKKLSEGTL
                     LSLDNQIDATTGTIKVKARFNNQDDALFPNQFVNARMLVDTEQNAVVIPTAALQMGNE
                     GHFVWVLNSENKVSKHLVTPGIQDSQKVVIRAGISAGDRVVTDGIDRLTEGAKVEVVE
                     AQSATTPEEKATSREYAKKGARS"
     gene            17060..20182
                     /gene="mdtB"
                     /locus_tag="RH057_001996"
     CDS             17060..20182
                     /gene="mdtB"
                     /locus_tag="RH057_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
                     /protein_id="HDV3128365.1"
                     /translation="MQVLPPSSTGGPSRLFIMRPVATTLLMVAILLAGIIGYRALPVS
                     ALPEVDYPTIQVVTLYPGASPDVMTSAVTAPLERQFGQMSGLKQMSSQSSGGASVITL
                     QFQLTLPLDVAEQEVQAAINAATNLLPSDLPNPPVYSKVNPADPPIMTLAVTSTAMPM
                     TQVEDMVETRVAQKISQISGVGLVTLSGGQRPAVRVKLNAQAIAALGLTSETVRTAIT
                     GANVNSAKGSLDGPSRAVTLSANDQMQSAEEYRQLIIAYQNGAPIRLGDVATVEQGAE
                     NSWLGAWANKEQAIVMNVQRQPGANIISTADSIRQMLPQLTESLPKSVKVTVLSDRTT
                     NIRASVDDTQFELMMAIALVVMIIYLFLRNIPATIIPGVAVPLSLIGTFAVMVFLDFS
                     INNLTLMALTIATGFVVDDAIVVIENISRYIEKGEKPLAAALKGAGEIGFTIISLTFS
                     LIAVLIPLLFMGDIVGRLFREFAITLAVAILISAVVSLTLTPMMCARMLSQESLRKQN
                     RFSRASEKMFDRIIAAYGQGLAKVLNHPWLTLSVALSTLLLSVLLWVFIPKGFFPVQD
                     NGIIQGTLQAPQSSSFANMAQRQRQVADVILQDPAVQSLTSFVGVDGTNPSLNSARLQ
                     INLKPLDERDDRVQKVIARLQTAVDKVPGVDLFLQPTQDLTIDTQVSRTQYQFTLQAT
                     SLDALSTWVPQLMEKLQQLPQLSDVSSDWQDKGLVAYVNVDRDSASRLGISMADVDNA
                     LYNAFGQRLISTIYTQANQYRVVLEHNTENTPGLAALDTIRLTSSDGGVVPLSSIAKI
                     EQRFAPLSINHLDQFPVTTISFNVPDNYSLGDAVQAIMDTEKTLNLPVDITTQFQGST
                     LAFQSALGSTVWLIVAAVVAMYIVLGILYESFIHPITILSTLPTAGVGALLALMIAGS
                     ELDVIAIIGIILLIGIVKKNAIMMIDFALAAEREQGMSPRDAIYQACLLRFRPILMTT
                     LAALLGALPLMLSTGVGAELRRPLGIGMVGGLIVSQVLTLFTTPVIYLLFDRLALWTK
                     SRFARHEEEA"
     gene            20183..23260
                     /gene="mdtC"
                     /locus_tag="RH057_001997"
     CDS             20183..23260
                     /gene="mdtC"
                     /locus_tag="RH057_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HDV3128366.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLNPQALFNQGVSLDDVRTAVSNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVSVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNIWLYISIPKTFFPEQDTGVLMGGIQA
                     DQSISFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQ
                     QIIDRLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRK
                     KLATLPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIY
                     QPMNQYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGL
                     SAASTISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILI
                     IAAIATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLI
                     GIVKKNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSG
                     GDGSELRQPLGITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            23261..24676
                     /locus_tag="RH057_001998"
     CDS             23261..24676
                     /locus_tag="RH057_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3128367.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLTIDGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHVSVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            24673..26076
                     /gene="baeS"
                     /locus_tag="RH057_001999"
     CDS             24673..26076
                     /gene="baeS"
                     /locus_tag="RH057_001999"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HDV3128368.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLQISAGQRDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            26073..26795
                     /gene="baeR"
                     /locus_tag="RH057_002000"
     CDS             26073..26795
                     /gene="baeR"
                     /locus_tag="RH057_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HDV3128369.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLPYVRQTPPDLILLDLMLPGIDGLTLCREIRRFSDIPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREHLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYGVGYRWEADACRIV"
     gene            26986..27318
                     /locus_tag="RH057_002001"
     CDS             26986..27318
                     /locus_tag="RH057_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDV3128370.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            27527..27823
                     /locus_tag="RH057_002002"
     CDS             27527..27823
                     /locus_tag="RH057_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HDV3128371.1"
                     /translation="MTYTDVRFLLTGGRKMARTMTVDLGDELREFIESLIESGDYRTQ
                     SEVIRESLRLLREKQAESRLQALRDMLAEGLSSGEAQPWEKDAFLRKVKAGIRK"
     gene            27825..28121
                     /locus_tag="RH057_002003"
     CDS             27825..28121
                     /locus_tag="RH057_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV3128372.1"
                     /translation="MRIIKLMPKANEDLEGIWYYSYHHFGEPQADRYVEHLSDVLQIL
                     SNNNIGTPRPELGEGIFVLPFERHVIYFLQSPGEIIVIRILNQNQDATRHLHWS"
     gene            28224..29585
                     /gene="yegQ"
                     /locus_tag="RH057_002004"
     CDS             28224..29585
                     /gene="yegQ"
                     /locus_tag="RH057_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HDV3128373.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIRNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPIAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(29915..>30163)
                     /locus_tag="RH057_002005"
     CDS             complement(29915..>30163)
                     /locus_tag="RH057_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128374.1"
                     /translation="ETSIGKFGLAYKSNIQRKLDNQYYTEAEASLARGRISGAENIVK
                     NDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFKCGE"
BASE COUNT         6967 a         8032 c         7971 g         7193 t
ORIGIN      
        1 ttcttaaata atgttttatt attaccactt ttaattcagg gataatgtga ggttattacc
       61 ctcaaataat atgagataaa tagtgctgca acattgcatt tttgccccga tttatccatg
      121 atcgaattgt gacatttgtc atacaacgaa taggttttgt acttactatg gaatggattg
      181 ccgatccgtc tatctgggcc gggttaatca cgctgattgt gatcgaactg gtcctcggca
      241 ttgataacct ggtctttatt gccatcctcg ccgaaaaact accgccgaag cagcgcgacc
      301 gcgcacgggt taccgggctg ctgctggcga tgttaatgcg cctgttactg ctggcgtcaa
      361 tctcctggct ggtcaccctg actcaaccgc tgttcagctt ccgctcgttt acctttagcg
      421 cccgcgactt aatcatgctg tttggtggtt tcttcctgtt gttcaaagcc acgatggagc
      481 tgaacgaacg gctggaaggg aaagacagca ataatcccac acaacgcaaa ggtgcgaagt
      541 tctggggcgt ggtgacgcaa attgtggtac tggacgccat tttctcactc gactcagtga
      601 ttaccgccgt cgggatggtc gaccatttac tggtcatgat ggccgccgtg gttatcgcta
      661 tcagcctgat gttgatggcc agcaagccgt taacgcaatt tgttaacagt cacccgacga
      721 tcgttattct ctgcttaagc ttcctgttga tgattggctt tagcctggtg gcagaaggtt
      781 tcggcttcgt cattccgaaa ggctacctgt acgctgccat tggtttctcg gtgatgatcg
      841 aggcgctcaa tcagttggct atctttaacc ggcgacgttt tctttccgct aaccagacgc
      901 tgcgccagcg gactaccgaa gcggtaatgc gcctgcttag cgggcaaaaa gaagatgcgg
      961 aactggacgc cgaaaccgcg tccatgctgg tggatcatgg taaccagcag atctttaatc
     1021 cgcaggaacg gcggatgatt gagcgggtac ttaatcttaa ccagcgtacc gtcagcagca
     1081 ttatgacgtc gcgccacgat attgagcata tcgatctcaa cgcgccggaa gacgagatcc
     1141 gccagttact ggagcgaaat cagcatacgc ggctggtggt taccgacggt gatgatgcag
     1201 aagatttgct cggtgttgtt cacgttatcg acctgctaca acagtcactg cgcggcgaac
     1261 cgctcaacct gcgggtgttg attcgccagc cgctggtgtt cccggaaacc ttgccgttgt
     1321 tacctgccct ggagcagttc cgtaatgccc gcacgcactt tgcttttgtg gtggatgagt
     1381 ttggctcggt ggaagggatt gtgacattaa gtgacgtcac tgaaaccatt gccggtaact
     1441 taccgaacga agtggaagag atcgacgccc gccatgatat tcagaagaat gcggacggtt
     1501 cctggacggc gaatggtcat atgccgctgg aagatctggt gcaatatgtg ccgctgccgc
     1561 tggatgaaaa acgtgaatat cacaccattg ccgggctgtt gatggagtat ttgcaacgta
     1621 ttccaaaacc tggcgaagaa gttcaggtgg gggattattt gcttaaaacg ttgcaggtgg
     1681 aaagccatcg cgtgcagaag gtgcagatta ttccactgcg taaggatggc gagatggagt
     1741 acgaggtgtg atggtatcgg tcattttacc cgatagccga aaggaataag atgagggaac
     1801 cgcgccagcg gcgatttacc gccgggagcc cgggttgcca gggtggtggc gattgagcca
     1861 ccctggcacg ttcaccggct tgagcgttcc atagtagtga ggaacatcaa gtgaacggaa
     1921 cagccttcga tcttacatat tcagaatata atggtgccgc cgcgaacgcc tgatgcgacg
     1981 ctttacgcgt cttatcaggc ctacaggaca ggtcacctgg taacctacat cttctccagc
     2041 aacttcttca catctttgcc attgcgggaa tctttattcc gctcggccca gtcattcagg
     2101 cgacgtttcg cttcgtcctg tagatgtttg cgcagtaact gatccacttg cagactgtaa
     2161 ttgagttgct gccagttgcc ataaacccgc agcggtactg gcgtttcttt gaggaaatca
     2221 atcagtttgc tttccccgtt ccagccaccc acgacccgaa tatcaaactg ggtgtcgcag
     2281 gtttgatctg ccagattcaa catgccttcc cctgtcagcg ccagcacagg cgattgacct
     2341 tgcatgtcgt ttaacgtcac gacgccatcc ttcaacgtca aatcggtggt aaagcggtca
     2401 agccgcgtca cgttatcaaa gttttcagcg gccttcacat caccgccatt acgctctacc
     2461 gcttgctgaa tcatctgctg gaagttcatc ccttccatgc gcgtgtcggt cattttgaca
     2521 tgtgcctgtc cttgccagtt gtggcggaat gcgtcggcat ctatgtcagc accggagaaa
     2581 tcaccagcca gtgacatttt tccggtcaac gaaatcggat agttaaacgc cttcagaatg
     2641 gtaccaatct caacgttttc cagccgtggc tggaagttta tccgcggatt tattgatgtc
     2701 gcgtccagcg tgcccggcag tgaaacctgt ccaccgttaa gcttgccctg cagttgagta
     2761 atttccagca aacccgactt gttggtcatt tgcgtggcaa catctgtaaa attcattccg
     2821 cgccagcgca cgttactggc ctgcaacaaa atatcagccg taaagccttg cagtccctga
     2881 tatgccggtt catcaatacg cgaagaaatg accgggcgcg gcaatgttga ttggctctgc
     2941 ccctgctgcg cggcaccgtt ttcaccattc gctgtttcat taagcgggat gaggttgtca
     3001 agattcagtt gcgggaattg cagtctcagc tgccattcag gtttctctgt cagcgtgacc
     3061 tgtgcttgcc cgctcagcgt gctgtcattg gcggtcaagc tgatttggtt aaatgaaagg
     3121 cgtttatgtg actcctgcca ctggacctgg aaactcccct gcccctgaat accttgtttt
     3181 ggtaaatctg caccctgcaa ctgccagtta atttgttcaa tagccgccgt taaatcatgc
     3241 ggataatcag aagcatctac cgtaccgtta agggatatcg tgagatcgcg ctgatcgcga
     3301 ttaacccgcc cggagaactc aaatgagcca cgatgttggg gatcttgttc catttgcagg
     3361 cggatattgc ggattgtcac ctgctcgtca tcttcatgct ggaacaccag cacgctgtcc
     3421 gccaccttaa gactggatat atcaaacgac catccgcgat cgtctgacag atccggcaag
     3481 gtattgtcgc gcggtgcaac cggagcgtct tcactgcgca ccgcttccgt ctgcggcgtc
     3541 agttggatca ctgccccttt tagcatcacc tgcttaacgc tcagttgatg actcagtagt
     3601 ggtaaaagcg ccacgtccag acgcatgttg tcggcgcgaa ccagtggctg gcttgcgccc
     3661 tgggcggtga gagacattcg cccggagagg atactaagct gcggccagac gtgccaacgc
     3721 agtggcccgt cgagctgcaa ttgataaccg ctacgtgcag caacttgctt gaccatatag
     3781 tcgcggaaat cattcggatt caccagcaac actaacgcag ataacccggc caccagcacg
     3841 accaggagta tcatcagcgt cgtcagaaat cgtctcatgg tatcctcaat gggcctgaat
     3901 tagtctttat cgattcggct ggctaccgcg ccctgctggt tgcgatattt cgcatcttca
     3961 cggcggttgt aaggtcgcgc cgccgggccg gaaagcggct caaagctcag cgcaccaatt
     4021 aacatgcccg gacgcagcgc cagcggcagc ttaccggagt tgtagaactc cagcacaatg
     4081 caaccagacc agcccggatc gatacggtgc gcggtgacgt gcaccatcag ccccagacgc
     4141 gccaaagagg aacgcccgtc cagccagccc accagatcgg ctggcagcgt caccgactcc
     4201 agcgtcaccg ccagcgccag ctctcctggg tgaagataga acgcctcgcc ctcgtcgaga
     4261 acgatctcat cgctcatcac gcggtcaagc gcggcgctca cttcatcttt gggaccgctc
     4321 agatcgataa acgctgccgt gtgaccacgg aaggtacgaa atttattgcc caggcgtaca
     4381 tccaccgtcg cgccgttaat acgctccact ggcggacgtg ggttgatcga caaacggccc
     4441 tcatcaagcc aggcttcaat atctcggtca cacagacgca tggcattttc tcctttcgcg
     4501 catcactccc ttaacgccaa ttacgtcaag ggcataccag gttatcactg aacggtacac
     4561 aatttatcaa gcttattcaa agaactgact tattttcgct ttcaatatat cgatcgcgat
     4621 gcggtttttc ccgccgcgcg gcacgataat gtccgcatat tgtttagaag gctcaatgaa
     4681 ttgcaggaac atcgggcgca cggttttttg atattgcgcc atcactgaat ccattgaacg
     4741 cccacgctcg ttaacgtcac gcttgatgcg gcgcatcagg cagatatcca gcggggtatc
     4801 aacgaaaatg gagaagttaa gttcgtcacg caaacgcgca tccgtcagca acaaaatgcc
     4861 ttcgagaatg atgaccttct tcggctcaac cgtcaccgtt tctttcatac gcgtatgttc
     4921 aacatagctg taaaccggca ggtcaattgc cgagccgcgt ttcaacgctt gtaaatgctc
     4981 aagcaacagg ctgtgatcca tcgcgctggg atggtcgtag ttggtcttaa cgcgttcttc
     5041 catcgacaga tggctttgat ctttgtaata gcagtcttcg ggaattacgc cgatgtgttc
     5101 atcaccgact tgctcacgta attcacgata aagggtactg gcaataagac tcttgccgga
     5161 agccgatgcg ccagcgatac cgataatgac gcactgatga gactgatcag tcatatattt
     5221 agcgacctga ttaacctgga tgttaggaag ggggcgacga agcgccaaac gcggcaatta
     5281 tagggatttc atccgcctga taccagtcga atagcgctgc cgcgcgctca gagttaattg
     5341 ttgacgaaga attcccggtg gcaaattacg ttgatcagtt ttatacaagg taaaaaatgt
     5401 tatacgcagt tgcgcaaatt atccgccttt acgtcacttt atgagcaatt cgcatataaa
     5461 atgtaaaact tttgtactag cataaacaca gaaacgaata ctggcgacct ggtcttgcgg
     5521 ataaagcggt aatgagcaaa caatcacagc atgtattaat tgccctgccc cacccgctgc
     5581 ttcacctggt cagtttaggt ttagtctcgt ttatctttac ccttttctcg cttgagcttt
     5641 cgcagtttgg cacccaactc gccccactgt ggttcccgac gtccatcatg atggtggcgt
     5701 tttatcgcca tgccgggcgc atgtggccgg gaattgcgct gagctgctcg ctgggaaata
     5761 tcgccgcatc catcctgctt ttttccacca gctcgctgaa catgacctgg acgaccatca
     5821 atattgttga agccgtggtc ggggcagtgc tgctacgtaa attgctgccg tggtataacc
     5881 ccttgcaaaa tctggctgac tggctgcgtc tggcattcgg cagcgccatt gttccgcctc
     5941 tgctaggggg tgttctggtt gtcctgctga cgcccggaga cgatcctctc agggcatttt
     6001 tgatatgggt actgtcagaa tccatcggcg ctctggcact ggtgccgctg ggattgttat
     6061 ttaaaccaca ctatctgctg cgccatcgca acccacggtt gctttttgag tcgctgctca
     6121 cgttagccat cacactgacg ttaagctggc tttcgatgct gtatctgccg tggcctttta
     6181 ctttcattat tgtgctgttg atgtggagcg ccgtgcgcct gccacgaatg gaagcctttt
     6241 tgatcttcct taccacggtg atgatggtgt cgctgatgat ggccgcggat ccctccctgc
     6301 ttgctacgcc gcgtacatac ctgatgagcc atatgccgtg gctaccgttt ttgctgatcc
     6361 tgctgcccgc caacatcatg acgatggtga tgtatgcctt tcgtgcggaa cgcaaacaca
     6421 tttccgaaag cgaaacccgt tttcggaacg ccatggaata ttccgctatc ggtatggcgt
     6481 tagtgggcac cgagggacaa tggctgcaaa ccaacaaagc gctctgccag tttctcgggt
     6541 acagtcagga agagctgcgc ggactcacct ttcagcaact gacctggccg gaggatctca
     6601 ataaagatct ccaacaggtt gaaaagctga taagcggtga aataaacacc tattcaatgg
     6661 aaaaacgcta ctacaaccgc aatggcgatg ttgtctgggc gttgcttgcc gtctcactgg
     6721 tgcgccacac ggatggcacg ccgctctatt ttatcgctca gattgaagac attaacgagc
     6781 taaaacgcac cgaacaggtg aatcagcaac tgatggagcg catcacgctg gctaacgaag
     6841 cgggcgggat tggcatctgg gagtgggagt tgaagccgaa tatttttagc tgggataagc
     6901 ggatgttcga gctgtatgaa attcctccac atatcaaacc gaactggcag gtgtggtacg
     6961 agtgcgtgct gccggaagat cgccagcatg ccgaaaaagt gattcgtgat tcgttgcaat
     7021 cacgctcgcc ctttaaactg gaatttcgca ttaccgtaaa agacggtatt cgccatatcc
     7081 gcgccctcgc caaccgggta ctgaataaag aaggcgaagt cgaacgcctg ctcggcatta
     7141 atatggatat gactgaggtt aaacagctta acgaggcatt gtttcaggaa aaagagcgcc
     7201 tgcacataac gctggactcc atcggcgaag ccgtggtctg tattgatatg gcaatgaaaa
     7261 ttacctttat gaatccagtg gcggagaaga tgagcggctg gacgcaggaa gaagcattag
     7321 gtgttccgct cctgacggtg ttgcatatta cctttggcga caatggtccc ctgatggaga
     7381 acatttacag tgccgacacc tcacgttccg cgattgaaca agatgtggtg ttgcactgcc
     7441 ggagcggcgg cagctacgac gtgcattaca gtattacgcc gttaagtact ctggacggca
     7501 gcaatattgg ttcggttctg gtgattcagg acgttaccga atcacgcaaa atgctgcgcc
     7561 agctgagcta cagcgcctcc catgatgcac tgacgcatct cgccaatcgc gccagttttg
     7621 agaagcaact gcgtatgctg ctgcaaacgg taaacagtac acatcagcga catgccctgg
     7681 tgtttatcga tcttgatcgc tttaaagcgg tgaatgacag cgccgggcat gcggcgggcg
     7741 acgctttgct gcgcgaactg gcgtcgttga tgctgagtat gctgcgttcc agcgacgtgc
     7801 tggcgcgact cggtggggat gaatttggtc tgctgctacc agactgtaat gttgaaagcg
     7861 cgcgttttat cgctacacgc attatcagtg ccgtgaatga ctatcacttt atatgggaag
     7921 gccgtgtgca tcgggtaggt gccagtgccg ggattacctt gattgatgac aacaatcatc
     7981 aggcggcaga agtgatgtcg caggctgata tcgcctgtta tgcctccaaa aatggtggac
     8041 ggggccgggt gacggtttac gaaccacagc aagctgccgc acatagcgaa cgggcggcga
     8101 tgtcgcttga tgaacagtgg cggatgatta aagagaatca gttgatgatg atggcccacg
     8161 gtgtcgcttc gccgcggatc ccggaagcgc gtaatttgtg gctgatttcg cttaaactct
     8221 ggagttgcga aggcgagatt attgatgaac aaaaatttcg tcgtagcttc agcgatccgg
     8281 cacttagcca tgctcttgac cgacgggtat tccacgaatt tttccagctg gccgcaaaag
     8341 cggttgccag taaaggcata agcatcgccc tccccctttc cgttgccggt ttgagtagcg
     8401 ccacgctggt gaatgatctg cttgagcagc tggaaaatag ccctctacca ccacggttat
     8461 tacatctgat tattccggca gacgcgattt tagatcacgc agaaagcgtg caaaaactgc
     8521 ggctggcggg atgtcggata gtgctcagcc aggtgggccg cgatctgcaa atcttcaact
     8581 cgctgaaagc gaatatggca gattacctgc tacttgatgg tgagttatgc gccaacgtgc
     8641 agggtaattt gatggatgag atgctgatta cgattattca ggggcacgct cagcgactcg
     8701 ggatgaaaac catcgccggg ccagtcgttt tacccttagt gatggatacg ctttctggca
     8761 tcggcgtcga tctgatttat ggtgatgtga ttgccgatgc ccaaccgctg gatttgctgg
     8821 tgaatagcag ttatttcgcg attaactgag ggagcgtttg cccattgcct gatgcgacgc
     8881 atcaggccta caattcgtcc gttccgtctg gttgccagcc ttccgtatac cagatatgca
     8941 acagcgcata agaacgccag ggcttccagc gctcggcata acggcggatt tgtgccggtg
     9001 tcatccccgg aaatcgctgt ttaatcagat aatcatccgg cagaaaaaca tctttcgcct
     9061 gccagccacg caaagcgaaa taattcgccg tccagcgccc gatacccgga aaagtttgca
     9121 gcgttttcat cgcctgctcc acatcgcccg gaattgtcat tggtaagctg ccctccagcg
     9181 ccgcatttgc cagatgaatc agtgcctctg cccgtttcaa cggcatgcct aacgctttta
     9241 atgcctgcgg gtcggctgct gccagccgct gaggcgtggg gaagcagata tactccggaa
     9301 aatcatccag ccgttcgcca taaagctgta caactttggc agtcaatttt gccgccatcg
     9361 ccacgctcac cagttggcct aaaatcgccc gcacaccctg ctcaaaagca tcaatacagc
     9421 cgggtaaacg caatccgggc cgcgccgcgc ctaacttgcc caacgcaccg ttaacaatct
     9481 gcgggttaca ttgcagatca aacaggcggc tcattttcgc cagacactca gcggcaacgg
     9541 gttctaaacc tgcacttaaa tttatgtgca gagtatggcg ggctatatcc ggaatagcag
     9601 tcaccacgcc gcgatattcg cccaccgcca gactacgggc ataataactg tccgcgaccg
     9661 tttccacacc actcactgca cgggcggcga gaaatcccaa catccacgac cagtcatacg
     9721 gcggctgcca gttcagggta tacatcgcat ctccttattc atccgctttc agcataaacg
     9781 ttattcagac gctacgcttt gctttcatat tccggttgtc gcgacagcaa catttcgcta
     9841 aagtcacgcc ccttcttcac cggcatgggg attaattcgt gtttattggt tttgattacg
     9901 gtacagcaaa ctgttcagtg gcggtcatgc gtgacggtaa accgcaatta ctgaaaatgg
     9961 aaaacgacag cacgctgctg ccttcaatgc tttgcgcgcc aacgcgtgaa tcggtaagcg
    10021 aatggctgta ccgccatcat gatgttccgg cagacgacga tgaaacgcag gcgctgttac
    10081 gtcgggcgat tcgttataac cgcgaagaag atatcgatgt tacggcgaaa agcgtgcagt
    10141 tcggtctttc ctcactggca cagtacattg atgatccaga agaagtgtgg tttgtgaaat
    10201 caccaaaatc gttcctcggt gccagcggct taaaaccgca gcaggtagcg ctgtttgagg
    10261 atctggtctg cgcaatgatg ttgcacattc gccagcaggc gcaggcgcag ctgccagaag
    10321 cgattactca ggcggtgatt ggtcgcccga tcaacttcca ggggctgggc ggcgatgaag
    10381 caaacgccca ggcgcaaggg attctggaac gcgcggcgaa gcgtgccgga ttcagggacg
    10441 tggtattcca gtacgagccg gtcgcggctg ggctggatta cgaagccacc ttgcaggaag
    10501 aaaaacgggt gctggtggtg gatatcggcg gtggtacgac tgactgttca ttgctgctga
    10561 tggggccgca gtggcgttcg cgtctcgatc gtgaagccag cctgctgggt cacagtggtt
    10621 gccgtattgg cggtaacgat ctggacatcg cactggcgtt taaaaacctg atgccgttac
    10681 tgggcatggg tggcgaaacc gaaaaaggca tcgccctgcc gattctgccg tggtggaatg
    10741 cggttgccat caacgacgta cctgcgcaga gtgatttcta cagtagtgcc aacggtcgtc
    10801 tgcttaacga tctggtacgc gatgcccgcg aaccggaaaa agtggccctg ctacagaaag
    10861 tctggcgtca gcgtttaagc tatcgcctgg tgcgtagcgc agaagagtgc aaaattgctc
    10921 tttcaagcgt agcggaaacc cgcgcctcac tgccgtttat cagcgatgaa ctggctacgc
    10981 tgattagcca gcaagggctg gaaagcgccc tcagccagcc actggcgcgg attctggaac
    11041 aggtgcaact ggccctggat aacgcccagg aaaaaccgga cgttatctac ctgaccggcg
    11101 gtagcgcccg ttcgccgctg attaaaaaag cgctggcaga acagttgccg ggaattccga
    11161 ttgcaggcgg cgatgacttt ggctccgtca ccgccgggct ggcacgctgg gcggaagtgg
    11221 tgtttcgtta aaagaaaatg gctcagcagc ggcttaaatc tgcctgcgcc tgagccagtt
    11281 tttctgccgc ctgacgtaac ggttccatca acggcatggt ccgtgctgga gcatgaaggc
    11341 ggaatcgctg caactctgtc gcgccgacag tcaacgtcga ttccagccga tgtcgtttgg
    11401 cggtcatttt cgccattacc gcttgtctgt ctgctggcgt tatcgcttca ttcggcctga
    11461 acacaaaacg gcgttcacag ctcgctttcc agtcgataac cgcctgggtc agatggtcgc
    11521 caaacccttt cacttgctta acgccacgac gggtaacatc tgcggctgtt tcaataccaa
    11581 aagaccgtaa cgccgcttta cgcgcagggc caacgccggg aatagaggca acatcaataa
    11641 aaaatccctc cagaaacttc tgcttctgcc gttcccttgc agtgtcatga agtgctgcca
    11701 gagcgcgttt ctcttcttcc ggtaacccga gaatttcgtc cttcattttt tcgagcatcg
    11761 tccgtttggc gataaaacct tccaggccgc ctacctgctg gatctggcgg actaaatggt
    11821 catagtccat tttcgcacga ttaaatgcct cacggcgttg ctgaacttct gctttgtacg
    11881 ctttgcttgt cagactgcca ataatccaga tagccgccag cacaggaacc aaaataatat
    11941 aacgcggttc tgcctgaagg ccgcaaagca gcagcgataa tgctgacagt gcgatctcaa
    12001 gcagaatgat gtattcgcgc cgtaacaggc ctaaaggaag cggcctgcca gtcggttgga
    12061 aatgatcggg taatggcagt tgcaatgctg gcggtgctac tgacgccatc accatcgccc
    12121 agacttttgc cagcacaaaa tccccgccgg tggtaatgac ctcttcgccg agatcaataa
    12181 aatagataac gccttgatta tccagcgcac accacgggca gtcggtcaaa tgagcgggat
    12241 aaacatgcat tgccgaaacg ggacatttct ttaactgttg gcgcagagaa tcaagtgccg
    12301 ctacccacgc tttagccgtc ggacgcccgg ttgccacgcc actttccgtg aatgcctgct
    12361 gaaacatggc ttcaacatca ctcggtaaca tcgatagcgg aatcgatcgt ggcggcggtt
    12421 ttaaaccacg tcgctgatta tctgacgcgt aggcataacg gaaatgggta atatccgtct
    12481 ccagcgcatt acccgcatca gagataagcg gcacaccaga ataaggatgc cgaccaccaa
    12541 acaagacgtg aaatatcagc aaggcaaggc caaaattatc gtgattcgcg gtgcgttcaa
    12601 agccgacaaa tgacgacaag gtttgtagct ctggcggcgt aaaatgcgac acaccgactt
    12661 cgcataaatg caacgtgcca ttagcgttaa tctgaaagga gtcgctatca atcaatacca
    12721 ctttgctgtc gcgacctacc ataaaactgt tctggtttac gtcacccacg acgtgcccgt
    12781 gctcgtgaac cgtagcaaaa gatgaagcaa tattgcgcgc aacatagagt agaaaatccc
    12841 acgcacaatg agggtaacgt tgacgacgat gtgccgggtt atagatcata tgaatcggtt
    12901 ctttaccaga aacttttggc atcataaaac cgataacttt tccgcctcgt ccaccgtgaa
    12961 gcgttgcctg cggccaggcg acataattca acaattgcgc gtcagctgtc gcagccataa
    13021 aggcaagttt gtcctgtttt aaggcgggtg gcggcgtgtg ataaatcttg gcgacgctat
    13081 cgacaaactc attgatatca taaaccgcgc cttcaccgcc tttgcccagt tcacggcctg
    13141 gcgtaacgca ctcaccagta gcagtatata aagtgggttt catggtaacc acagcgccag
    13201 tgccaacgtt ttatcatcat ccgtacgctc gtttaccgcc gggctggaca aaaactgctt
    13261 taacaattca ggtaataaat cgagttgttc ctgcgttgct gacgccagtc cattgaaaaa
    13321 cggggtaaag aaaggtacat gaggagaatt atccagcata tttaacgcca gtcgctggat
    13381 accatccgta aatgctgcaa ctttatgcac acgctcagtg ctggtgaaag tttccagtct
    13441 ggaaacggca tcttcatcag taatgaagtg cgtcatattg gcatattcac ccaccatcgg
    13501 cgtaagcggc aattgcagac cgtgaccaaa atccaccacc acgccgccat cgccaatctg
    13561 cataatcaag gtgccattag ccgatgaaat caatcccaga aacgtgcagg caaaatcgcg
    13621 aactgctaat tctttggcct ctgcctcggc aaaaagtcgc tgacgaatag tcagcacaat
    13681 atccgtggcg aggatatcat taagccccaa ttccccaccc tgcacttttt gcgacatata
    13741 agccatcgct tcattgacgg cgagcatcgc gccttcgcca ccctgcgaga cgctgcctgc
    13801 accatcagca agaaacatca ccagtaatgg ttgctgatcg tttaaccagg caacctgcat
    13861 ctggcaggca tcctgacaag gtaaatcagc gctaatgtgc gacgttccaa cagcggatgc
    13921 ataaaccaga cgccagctca cactgacgtc cagccttttg gcgcttccag cacaacttcc
    13981 gttcccggcg tggagcggga aaccgaacgc agcgaactgg ataaccagct aaacagttca
    14041 cggaattgta atccttgcag aggcaaaggc tgacgaacgc tgatttgtgc cagcgtcttc
    14101 atatccgccc cctgaacacc gatggaaaag aaggcaaact tcttatcttc ttccccctga
    14161 aacactttgt tggcggcagc ctgccactca tcggttggag cgccatcggt gatcaggaaa
    14221 atccacgggc gataatacga aataccattt gcgcggtact cacgtttcct ctcttcaacc
    14281 atatccaggg ctttggtaat ggcggccccc attggcgtat ccccctgggc aaacaggatg
    14341 ggcgggaaaa agttagcggc gctggtaaag ggttgttcca catgcaccgg gccgaacgtc
    14401 acaatgccga gttccactct ttttaaagcc agcggatcgg caagcagttc atcacgaaag
    14461 gccaccaatc cggcgttaag ttcattaata ggtctgccgc tcatcgagcc agaaacatcc
    14521 agcagcagaa tgcaggggca acgtggttcc gggttactgg caaaatcgct ggtggcaaac
    14581 gtgatttgtt cgctcataag aaatcctttc ttagagaatt atcgaggtcc ggtatcatac
    14641 cgactgcaat aatgaaataa gagcaaacga agaaactttc agaattaggc ccggcggcgc
    14701 agaaaatttt gctattcgcc tcgcaagaac ggagagccgt cgattgacac taagggcgga
    14761 gtgacataat ttcaggagtg agggttaggg agaggtttcc ccctccccct ggtgttctta
    14821 gtaagcctgg aagctaatca ctaagagtat caccagtatg atgacgtgct tcatcataac
    14881 cctttcctta ttaaaagccc tcttctccgg gagaggcttt cccgtttcag cgtcccgctg
    14941 aaatcatcgg cttacctcct ttcgccctga atgcagtcta tcgctaacgc gtgaataaaa
    15001 tagtttcctc tgtgattatt gcgtaacgac tcgcaaagaa tatttgctgg caggatcgca
    15061 gactacaaag cctgcggatt gacaatctta tcgtgaaggc atactttcag gagtgagggt
    15121 agagcggggt ttcccccgcc ctggtagtct tagtaagcgg ggaagcttat gactaagagc
    15181 accacgatga tgagtagctt catcatgacc ctttccttat ttaaggcccc ttcctcggga
    15241 ggggctttcc cgtttcagcg tcccgctgaa atcgtcggct tacctccttt cgccatgcac
    15301 ttagtctatc gctgacttgc agataaaata gtttccagtg tgattattgc gtaacgtctc
    15361 gcaaagaatt tttgctggca ggatcgcaga cggcaaagcc tgcggattga caatcttatc
    15421 gtgaaggcat actttcagga gtgagggtag agcggggttt cccccgccct ggtagtctta
    15481 gtatgctggg tagctaatca ctaagagcac cacgatgatg agtagcttca tcatgaccct
    15541 ttccttattt aaggcccctt cctcgggagg ggctttcccg tttcagcatc cggctgaaat
    15601 ctgcggctta cctcctttcg ccctgtatgt agtctatcgc taacgcgtag ataaaatagt
    15661 ttcctgtgtt attactggat gcgtgctcgc aaatgtgccc gccattcaga cgattcccga
    15721 tagtgtttca taattcctcc atttttctcc cttattggct ggctacacta gtatcattcc
    15781 gcgaaacgtt tcaggaagag aaactcttaa cgatgaaagg cagttataaa tcccgttggg
    15841 taatcgtaat cgtggtggtt atcgccgcca tcgccgcatt ctggttctgg caaggccgca
    15901 atgactccca gagtgcagcc ccaggggcga cgaaacaagc gcagcaatcg ccagcgggtg
    15961 gtcgccgtgg tatgcgttcc ggcccattag ccccggttca ggcggcgacc gccgtagaac
    16021 aggcagttcc gcgttacctc accgggcttg gcaccattac cgccgctaat actgttacgg
    16081 tgcgcagccg cgtggacggt caactgatgg cgttacattt ccaggaaggc cagcaggtca
    16141 aagcaggcga tttactggca gaaattgacc ccagccagtt caaagttgca ttagcacaag
    16201 cccagggcca actggcaaaa gataaagcca cgcttgctaa cgcccgccgt gatctggcgc
    16261 gttatcagca actggcaaaa accaatctcg tctcccgtca ggagctggat gcccaacagg
    16321 cgctggtcag tgaaaccgaa ggcaccatta aggctgatga agcaagcgtc gccagcgcgc
    16381 agctgcaact cgactggagc cgcatcaccg caccagtcga tggtcgcgtt ggtctcaagc
    16441 aggttgatgt tggtaaccaa atctccagtg gtgataccac cggaattgtg gtgatcaccc
    16501 agacgcatcc tatcgattta gtctttaccc tgccggaaag cgatatcgct actgtagtgc
    16561 aggcgcaaaa agccggaaaa ccgctggtgg tagaagcctg ggatcgcacc aattcgaaga
    16621 aattaagtga aggcacgctg ttaagcctcg ataaccaaat cgatgccaca accggtacga
    16681 ttaaagtgaa agcacgcttt aataatcagg atgatgcgct gtttcccaat cagtttgtta
    16741 acgcgcgcat gttagtcgac accgaacaaa acgccgtagt gatcccaaca gccgccctgc
    16801 aaatgggcaa tgaaggccat tttgtctggg tgctgaatag cgaaaacaag gtcagcaaac
    16861 atctggtgac gccgggcatt caggacagtc agaaagtggt gatccgtgca ggtatttctg
    16921 cgggcgatcg cgtggtgaca gacggcattg atcgcctgac cgaaggggcg aaagtggaag
    16981 tggtggaagc ccagagcgcc accactccgg aagagaaagc caccagccgc gaatacgcga
    17041 aaaaaggagc acgctcctga tgcaggtgtt acccccgagc agcacaggcg gcccgtcgcg
    17101 cctttttatt atgcgtcctg tcgccaccac gctgctgatg gtggcgatct tactcgccgg
    17161 gattatcggt tatcgcgccc tgcccgtttc ggcgctgccg gaagtggact atccgaccat
    17221 tcaggtggtc acgctctacc caggtgccag cccggatgtc atgacctctg ccgttaccgc
    17281 gccgctagaa cgccagttcg ggcagatgtc tggcctgaaa cagatgtcgt cgcaaagttc
    17341 cggcggtgcg tcagttatca ctttgcagtt ccagctaaca ttaccgctcg atgtcgccga
    17401 gcaggaagtg caggccgcga ttaacgctgc gaccaacttg ttgccgagcg atctgcctaa
    17461 cccgccggtt tacagcaaag tgaacccggc agatccgccg atcatgacgc tcgccgtcac
    17521 ctcaaccgcc atgccgatga cgcaagtgga agatatggtg gaaacccgcg tcgcgcagaa
    17581 aatctcgcag atttccggtg tcggactggt gacgctttcc ggcggtcagc gtccggctgt
    17641 tcgcgtcaaa cttaacgctc aggcgattgc cgccctcggc ctgaccagcg aaaccgtgcg
    17701 caccgccatt accggcgcta acgttaactc ggcaaaaggt agcctcgacg gcccttcccg
    17761 tgcggtcacg ctttccgcta acgaccagat gcaatccgcc gaagagtatc gccagctaat
    17821 catcgcctac cagaacggcg caccgattcg tctgggcgat gtcgcaaccg tagagcaagg
    17881 agcagaaaac agctggctcg gcgcgtgggc gaacaaagaa caggccattg tgatgaatgt
    17941 tcagcgccag cccggtgcta acattatctc caccgccgac agcatccggc agatgctgcc
    18001 acagctcacc gagagtctgc cgaaatcggt gaaggtgaca gtgctttccg atcgcaccac
    18061 caatattcgc gcatccgtcg atgatactca gtttgaattg atgatggcta tcgcgctggt
    18121 agtcatgatc atctacctgt ttttgcgcaa tattccggcg accatcattc ccggtgttgc
    18181 tgtaccgctg tcgttaatcg gcacttttgc ggttatggtg tttctcgatt tttcaatcaa
    18241 taacctgaca ctgatggcgt taactatcgc caccggattc gtggtcgatg acgccatcgt
    18301 agtgatcgaa aacatttccc gctatatcga aaaaggcgaa aaaccgttgg cggcggcgct
    18361 caaaggcgcg ggtgaaatcg gctttaccat tatctcgctg accttctcac tgattgcggt
    18421 gttgatccca ctgctgttta tgggcgatat cgtcgggcga ctgttccgcg aatttgctat
    18481 taccctggcg gtagcgattt tgatctcagc ggtggtgtcg ctgaccctga caccgatgat
    18541 gtgcgcgcgg atgctcagcc aggagtcgtt gcgtaaacag aaccgcttct cccgtgcctc
    18601 ggaaaaaatg ttcgacagga taatcgccgc ctatggtcag ggacttgcga aagtgctgaa
    18661 tcatccgtgg ctgaccttaa gcgtggcact cagcacgctg ctgcttagcg tgctgctgtg
    18721 ggtgttcatt ccgaaaggtt tcttcccggt acaggacaac ggcattattc agggcacttt
    18781 gcaggcaccg caatccagct cctttgccaa tatggcccag cgacaacgcc aggtcgcgga
    18841 cgtgattttg caggatccag cggtgcaaag cctgacctca tttgttggtg ttgatggcac
    18901 taacccgtcg ctgaatagtg cacgtttaca aatcaacctc aaaccgttgg atgaacgtga
    18961 tgatcgggtg caaaaagtca tcgcccgtct gcaaacggcg gtagataaag tgccgggcgt
    19021 cgatctcttc ctgcaaccaa cgcaggacct gaccatcgat actcaggtca gccgcaccca
    19081 gtaccagttt accttgcagg ccacgtcact ggatgcgctc agtacctggg tgccacagtt
    19141 gatggaaaaa ctccagcaac tgccacagct ttctgatgtc tccagcgact ggcaggacaa
    19201 agggctggtg gcgtatgtca atgttgatcg cgacagcgcc agccgtctgg ggatcagcat
    19261 ggcggacgtc gataacgccc tgtacaacgc gtttggtcag cgactgattt ccactattta
    19321 tactcaggct aaccagtatc gcgtggtgct ggagcacaat accgaaaata ccccaggcct
    19381 cgcggcgctg gataccattc gcctgaccag cagcgacggt ggcgtggtgc cgttaagctc
    19441 aattgccaaa attgagcagc gttttgcgcc gctttccatc aaccatctgg atcagttccc
    19501 ggtaacgacc atctccttta acgtgccgga taactattcg ctgggcgatg cggtgcaggc
    19561 gattatggac accgaaaaga cgctgaatct gccggtggat atcaccacgc agttccaggg
    19621 cagcaccctc gccttccagt cggcgctggg cagcactgtc tggctgattg tcgcggcggt
    19681 ggtggcgatg tatatcgtgc tcggcattct gtacgagagc tttattcacc cgatcaccat
    19741 tctctcgacg ctacccaccg caggagtcgg cgcactgctg gcgttaatga ttgccggtag
    19801 cgaactggat gtgattgcga ttatcggcat tattttgctg atcggtatcg tgaagaagaa
    19861 cgccatcatg atgatcgact tcgcgctggc tgctgagcgc gagcaaggca tgtcgccgcg
    19921 cgatgccatc taccaggctt gtctgttgcg ttttcgtccg atcctgatga ccactctggc
    19981 ggctctgctt ggcgcgctgc cgctgatgtt gagtaccggg gtcggcgcgg aactgcgtcg
    20041 tccgttaggt atcggcatgg tcggcggcct gattgtcagc caggtgctga cgctgtttac
    20101 cacgccggtg atttatttgc tgttcgaccg cctggcattg tggaccaaaa gccgctttgc
    20161 ccgtcatgaa gaggaggcgt aagtgaagtt ttttgccctc ttcatttacc gtccggtggc
    20221 gacgatttta ctgtcggttg ccattaccct gtgcggcata ctgggcttcc gtatgctgcc
    20281 ggtcgccccg ctgccgcagg tcgattttcc ggtgattatg gtcagcgcct cgctgcccgg
    20341 tgcgtcacca gaaacaatgg cgtcttccgt tgccacgccg ctggagcgct cacttgggcg
    20401 cattgccgga gtcagtgaaa tgacctccag cagttcgctc ggcagcacgc gtattatttt
    20461 gcagtttgat tttgaccggg atatcaacgg cgcagcgcgt gatgtgcagg cggcgatcaa
    20521 cgctgcacaa agtttgctgc ccagtgggat gcccagccgc ccgacctatc gcaaagcgaa
    20581 cccgtcggat gcgccaatta tgatcctcac gctgacgtcc gatacttatt cgcagggtga
    20641 actgtacgat ttcgcctcga cgcagctggc tccgacgatt tcgcaaatcg acggtgttgg
    20701 tgatgtcgat gtcggaggca gctcactgcc cgccgtacgc gtcgggctga atccgcaggc
    20761 gctgtttaat cagggcgtgt cgctggacga cgtacgcacc gccgtcagca atgccaacgt
    20821 gcgtaaaccg cagggcgcgc tggaagatgg cactcaccgc tggcagatcc agaccaatga
    20881 tgagctaaaa accgccgctg aatatcagcc gttgattatt cactacaaca acggcggcgc
    20941 ggttcgtctg ggcgatgtgg cgacggtgac cgactcagtg caggatgtgc gcaacgccgg
    21001 gatgaccaac gccaaaccgg ctattttact gatgatccgc aaactgccgg aagccaatat
    21061 tatccagacg gttgacagca tccgggcaaa attaccggag ttgcaggaaa ccattccggc
    21121 ggcgattgat ctgcaaattg cccaggatcg ctcccccacc attcgcgcct cgctggaaga
    21181 agtcgagcaa acgctgatta tctcggtggc gctggtgatt ctggtggtgt ttttattcct
    21241 gcgctcgggt cgcgccacta ttattcccgc cgtttcggtg ccggtttcgc tgattggtac
    21301 gtttgcggcg atgtacctgt gcggattcag tctcaataac ctttcgttaa tggcgctcac
    21361 catcgctact ggtttcgtgg tggatgacgc catcgtggtg ctggaaaaca ttgcacgtca
    21421 tctggaagcg ggaatgaaac cgttgcaagc cgcactgcaa ggtactcgcg aagtcggttt
    21481 tacggtgctg tcgatgagtc tgtcactggt ggcggtgttc ctgccgctgc tgttgatggg
    21541 cggattgccg ggccgactgt tacgcgaatt tgccgtgacg ctttctgtcg ccattggtat
    21601 atcgttgctg gtttctctga cattaacgcc aatgatgtgt ggctggatgc tgaaagccag
    21661 caagccgcgc gagcaaaagc gactgcgtgg ttttggtcgc atgttggtag ccctgcaaca
    21721 aggctacggc aagtcactga aatgggtgct caatcatacc cgtctggtgg gcgtggtgct
    21781 gcttggcacc attgcgctga atatctggct gtatatctcg atcccgaaaa ccttcttccc
    21841 ggagcaggac actggcgtgt tgatgggcgg gattcaggcg gatcagagta tttcgtttca
    21901 ggcgatgcgc ggtaagttgc aggatttcat gaaaattatc cgtgacgatc cggcagtgga
    21961 taatgtcacc ggctttacag gcggttcgcg agtgaacagc gggatgatgt ttatcaccct
    22021 caagccacgc gacgaacgca gcgaaacggc gcagcaaatt atcgaccgtc tgcgcgtaaa
    22081 actggcgaaa gaaccggggg cgaatctgtt cctgatggcg gtacaggata ttcgcgttgg
    22141 tgggcgtcag tcgaacgcca gctaccagta cacgttgtta tccgacgacc tggcggcact
    22201 gcgcgaatgg gagccgaaaa tccgcaaaaa actggcgacg ttgccggaac tggcggacgt
    22261 gaactccgat cagcaggata acggcgcgga gatgaatctg gtttacgacc gcgacaccat
    22321 ggcacggctg ggaatcgacg tacaagccgc caacagtctg ttaaataacg ccttcggtca
    22381 gcggcaaatc tcgaccattt accagccgat gaaccagtat aaagtggtga tggaagtgga
    22441 tccgcgctat acccaggaca tcagtgcgct ggaaaaaatg ttcgttatca ataacgaagg
    22501 caaagcgatc ccgctgtcgt atttcgctaa atggcaaccg gcgaatgccc cactatcggt
    22561 gaatcatcag ggattatcgg cggcctcgac catttcgttt aacctgccga ccggaaaatc
    22621 gctctcggac gccagtgcgg cgatcgatcg cgcaatgacc cagcttggtg tgccttcgac
    22681 ggtgcgcggc agttttgccg gcacggcgca ggtgttccag gagacgatga actcgcaggt
    22741 gatcctgatt atcgccgcca tcgccacggt gtatatcgtg ctgggtatcc tttacgagag
    22801 ttacgtacat ccgctgacga ttctctccac cctgccctcg gcgggcgttg gagcgctgtt
    22861 ggcgctggag ctgttcaatg ccccgttcag cctaatcgcc ctgataggga tcatgctatt
    22921 aatcggcatc gtgaagaaaa acgccattat gatggtcgat tttgcgcttg aagcccaacg
    22981 gcacggtaac ctgacgccgc aggaagctat tttccaggcc tgtctgctgc gttttcgccc
    23041 gattatgatg actaccctgg cggcgctgtt tggtgcgctg ccgctggtat tgtcgggcgg
    23101 cgacggctcg gagctgcggc aacccctggg gatcaccatt gtcggcggac tggtaatgag
    23161 ccagctcctt acgctgtata ccacgccggt ggtgtatctc tttttcgacc gtctgcggct
    23221 gcgtttttcg cgtaaaccta aacaaacggt aaccgagtaa atgacagatc ttcccgacag
    23281 cacccgttgg caattgtgga ttgtggcttt cggcttcttt atgcagtcgc tggacaccac
    23341 catcgtaaac accgcccttc cctcaatggc gcaaagcctc ggggaaagtc cgttgcatat
    23401 gcacatggtc attgtctctt atgtgctgac cgtggcggtg atgctgcccg ccagcggctg
    23461 gctggcggac aaagtcggcg tgcgcaatat tttctttacc gccatcgtgc tgtttactct
    23521 cggttcactg ttttgcgcgc tttccggcac gctgaacgaa ctgttgctgg cacgcgcgtt
    23581 acagggcgtt ggcggcgcga tgatggtgcc ggtcggcaga ttgacggtga tgaaaatcgt
    23641 accgcgcgag caatatatgg cggcgatgac ctttgtcacg ttacccggtc aggtcggtcc
    23701 gctgctcggt ccggcgctcg gcggtctgct ggtggagtac gcgtcgtggc actggatctt
    23761 tttgatcaac attccggtgg ggattatcgg tgcgatcgcc acattgatgt taatgccgaa
    23821 ctataccatg cagacgcggc gcttcgatct ctccggcttt ttattgctag cggttggcat
    23881 ggcggtatta accctggcgc tggacggcag taaaggtaca ggtttatcgc cgctgacgat
    23941 tgatggcctg gtcgcagttg gcgtggtggc actggtgctt tatttgctgc acgccagaaa
    24001 taacaaccgc gctcttttca gtctgaaact gttccgcact cgtacctttt cgctgggcct
    24061 ggcggggagc tttgccggac gtattggcag tggcatgttg ccctttatga caccggtttt
    24121 cctgcaaatt ggcctcggtt tctcgccgtt tcatgccgga ctgatgatga tcccgatggt
    24181 gcttggcagc atgggaatga agcgaattgt tgtacaggtg gtgaatcgct ttggttatcg
    24241 tcgggtactg gtagcgacca cgctgggtct gtcgctggtc accctgttgt ttatgactac
    24301 cgctctgctg ggctggtact acgttttgcc gttcgtcctg tttttacaag ggatggtcaa
    24361 ctcgacgcgt ttctcctcca tgaacaccct gacgctgaaa gatctcccgg acaatctggc
    24421 gagcagcgga aacagcctgc tgtcgatgat tatgcaattg tcgatgagta tcggcgtcac
    24481 catcgccggg ctgttgctgg gactttttgg ttcgcagcat gtcagcgtcg acagcggcac
    24541 cacacaaacc gtctttatgt acacctggct tagcatggcg tttatcatcg cccttccggc
    24601 gttcatcttt gccagagtgc cgaacgatac gcatcaaaat gtagctattt cgcggcgaaa
    24661 aaggagcgcg caatgaagtt ctggcgacct ggtattaccg gcaaactgtt tctggcgatt
    24721 ttcgccacct gcattgtctt gctgatcagt atgcactggg cggtgcgtat cagttttgag
    24781 cgtggcttta ttgattacat caagcatggt aatgaacagc gattacaact gttaagtgat
    24841 gcgcttggcg agcagtatgc gcagcatggc aactggcgct tcctgcgcaa caatgatcgc
    24901 tttgtctttc agatcctgcg ttcatttgaa cacgataatt cggaagataa acccggcccg
    24961 ggtatgccac cgcacggctg gcgtacccag ttctgggtgg ttgatcaaaa caacaaagtg
    25021 ctggttggtc cgcgagcgcc gattccacct gacggtacac ggcgacccat tctggtcaac
    25081 ggtgcggaag ttggcgcggt gatcgcctcc cccgttgagc ggttaacgcg caatactgat
    25141 atcaatttcg ataaacaaca gcggcaaacc agctggttga ttgtcgccct ggcaacgtta
    25201 ctcgcggcac ttgccacttt tctgctggcg cgcggtttac tggcaccggt aaaacgactt
    25261 gtcgatggca cgcacaaact ggcggcgggc gatttcacta cccgcgtaac gcccaccagt
    25321 gaagatgaac tgggcaaact ggcgcaagac ttcaaccagc ttgccagcac actggagaaa
    25381 aaccagcaaa tgcggcgcga ttttatggcc gatatttctc acgaactgcg tacgccatta
    25441 gcggtgctgc gcggtgaact ggaagccatt caggatggcg tgcgtaaatt cacgccggag
    25501 acggtggcgt ctttacaggc ggaggtcggt acactgacca aactggttga cgatctccat
    25561 cagttgtcga tgtctgatga aggcgctctc gcctatcaaa aagcaccggt agatttgatc
    25621 ccactgctgg aagtggcggg cggcgcattt cgcgaacgat tcgccagtcg tggcctgaaa
    25681 ctgcaatttt ccctgccaga cagtattacc gtatttggcg atcgcgaccg tttaatgcag
    25741 ttattcaata acttactgga aaacagcctg cgctacactg acagcggcgg cagcctgcaa
    25801 atctctgccg ggcagcgcga caaaacggtg cgcctgacct ttgccgacag tgcgccaggt
    25861 gtcagtgacg atcagctaca aaaattgttt gaacgttttt atcgcaccga aggttcccgc
    25921 aaccgtgcca gcggcggttc cgggctgggg ctggcgattt gcctgaacat tgttgaagca
    25981 cataatggtc gcattattgc tgcccattcg ccttttggcg gggtaagcat tacagtagag
    26041 ttaccgctgg aacgggattt acagagagaa gtatgaccga gttaccaatc gacgaaaaca
    26101 caccgcgtat tttgatcgtg gaagatgaac cgaagctggg gcagttgctc attgattatc
    26161 tgcgtgctgc gagctatgcg ccgacgctta tcagccacgg cgatcaggta ctgccgtatg
    26221 tgcgccagac accaccggat ctgatcctgt tggatctgat gctccctggc atcgatggct
    26281 tgacgctgtg ccgggaaatt cgtcgttttt ctgacattcc tatcgtgatg gtgacggcaa
    26341 aaatcgaaga gatcgaccgc ctgctcggac tggagattgg cgcagatgac tacatctgca
    26401 agccgtacag cccacgggaa gtggtagcgc gcgtcaaaac cattttgcgc cgttgcaaac
    26461 cgcagcgcga gttgcagcaa caggatgctg aaagcccgtt gattatcgac gaaggtcgtt
    26521 ttcaggcttc atggcgcggt aaaatgcttg acctgacacc tgcggaattt cgtctgctga
    26581 aaacgctctc tcatgaacca ggaaaagtgt tctcccgcga gcatttgctc aatcatcttt
    26641 atgacgacta ccgcgtagta accgaccgca ccatcgacag ccacattaaa aacctgcgcc
    26701 gcaagctgga atctctcgac gccgaacagt catttatccg cgccgtttac ggcgttggtt
    26761 accgctggga agccgacgcc tgccgcatcg tttagtttta gcgacatgac tttgttagcc
    26821 ggagatggcg ttcagaccag atccggcaac attatcccac gcatggtcag caaactgaca
    26881 ttatctctcc gccctggcag gcatcagaaa atcgctcata ctttaatcgg taaacagcac
    26941 ctttagatgc tgttttcgat acgcaatttc aatcaaggag tcattatggc tggttggttt
    27001 gaactcagta agagcagtga taatcagttc cggtttgtgc taaaagcggg caatggtgag
    27061 actatcctca ccagcgagct gtatacctca aaagcctctg cggaaaaggg catcgcgtcg
    27121 gtgcgtagca acagcccgca agaagaacgc tatgagaaaa aaacggcaag taacggcaaa
    27181 ttctatttca atctgaaagc cgctaatcat caaattatcg gctccagcca gatgtacgcc
    27241 accgcgcaat ctcgtgaaac cggaattgcc tccgttaaag ccaatggcac aagccagacg
    27301 gtgaaagaca atacgtaatt acgatgccgg gcgtgggttg ttggcgtccg gctttatatc
    27361 caaataacgc ttagttatta ataattaaga gtaaccgccg ccattaaaat agtgggcact
    27421 gacgagcact cgttgtgctt tctttgttta ccatcccata cttatcctcc tgaacatgtt
    27481 catgtgccaa aatatcttct aatactttcg tgacgctatc cggtttatga catacactga
    27541 tgtaagattt ttacttacag gcgggcgtaa aatggccaga acgatgacgg ttgatctcgg
    27601 cgatgagttg cgtgaattta tcgagtcctt aattgaatcc ggcgactatc gcacccaaag
    27661 cgaagtgatc cgtgagtcac tgcgattact tcgagaaaag caagcagagt ctcgcctgca
    27721 agcgttacgt gacatgctcg cagaagggtt gagtagtggt gaagctcagc cgtgggaaaa
    27781 ggacgcgttc ttacggaagg tgaaagcagg gataaggaaa taatgtgcgt attattaaac
    27841 tcatgccaaa agccaatgag gatttagaag gcatctggta ttacagctat caccatttcg
    27901 gcgaaccaca agccgacaga tatgtggaac atctttcaga cgttctccag attctgagta
    27961 acaataatat aggtacgccc agacctgaat taggcgaagg tatttttgtt cttccttttg
    28021 aacgccacgt catttatttc ctgcaatcac caggggaaat tatcgtcatt cgtatcctta
    28081 accaaaacca ggacgccacc agacatttac actggagcta aacccgccga ttgcgctaca
    28141 atgcccgccc ttaaagtggg ggcactcccc taaccgcttc atcaggtgaa gcggatctga
    28201 cctgtcatca gaacgagaga attatgttta aaccggaact cctttccccg gcgggaacgc
    28261 tgaaaaatat gcgttacgct ttcgcttatg gcgcagatgc tgtttatgcg ggccagccgc
    28321 gttattccct gcgtgtgcgc aacaacgaat tcaaccacga aaatcttcag ctcggcatca
    28381 atgaagccca cgcgctgggg aaaaagtttt atgtcgtggt caacattgca ccgcacaacg
    28441 ccaagctgaa aacctttatc cgtgacctga aaccggtggt ggaaatgggg ccggatgcgc
    28501 tgattatgtc cgatccaggg ctgattatgc tggtgcgtga gcacttccct gaaatgccga
    28561 tccacctttc ggtgcaggct aacgccgtga actgggcgac ggtgaaattc tggcagcaaa
    28621 tgggcctgac ccgcgtgatc ctctctcgcg agctgtcgct ggaagagatt gaagagatcc
    28681 gcaatcaggt gccggatatg gagatcgaga tcttcgttca cggcgcgctg tgcatggcct
    28741 actccggtcg ctgcctgctc tctggctata tcaacaagcg cgacccgaac cagggcacct
    28801 gcaccaacgc ctgccgctgg gagtacaacg tccaggaagg gaaagaagat gatgttggca
    28861 acatcgtaca caagtacgag ccgattccgg tgcaaaatgt tgagccgacg ctgggtatcg
    28921 gcgcaccaac cgacaaagtg tttatgatcg aagaggccca gcgtccgggc gagtatatga
    28981 ccgcgtttga agatgagcac ggcacttaca tcatgaactc gaaagatctg cgcgccatcg
    29041 cccatgtaga acgcctgacc aaaatgggcg tgcattcgct gaaaatcgaa ggtcgtacca
    29101 aatctttcta ctattgtgca cgcaccgcac aggtttaccg caaagctatc gatgacgccg
    29161 ctgcgggaaa accgttcgat accagcctgc tggaaactct ggaaggtctg gcgcatcgtg
    29221 gctataccga aggtttcctg cgtcgtcata ctcacgacga ttatcagaac tacgaatacg
    29281 gttattcagt ttctgaccgc cagcagtttg ttggtgagtt taccggtgag cgcaaggggg
    29341 acctcgcggc ggtagcggtg aaaaataaat tctccgttgg cgacagcctt gagctgatga
    29401 cgccgcaagg caacattaat tttacccttg agcacatgga aaacgccaaa ggcgaagcta
    29461 tgccgatagc accaggcgat ggttatactg tgtggctccc ggtcccgcag gatcttgagc
    29521 tcaattacgc gctgctgatg cgtaatttct ccggggaaac cacgcgtaat ccccacggta
    29581 agtgattaat ttcgattatt tttcccggat ggaaaattct tagaaaccga tcacatacag
    29641 ctgcatttat taaggttatc atccgtttcg ctgaaaaaca taacccataa aatgctagct
    29701 gtaccaggaa ccacctcctt agcctgtgta atctccctta cacgggctta ttttttatgc
    29761 ataagcccta tccctggtca ccgtcttcca ttgaccacat cgatagaatc tcccttcata
    29821 gcacgatgcc tttcacgtaa cggcatcgtg ctcgcacagg ttccggctaa gcacaaccag
    29881 aacgcgcatg tttgatgctt accaaaaaat attctcactc tccacatttg aatgtcagac
    29941 gagcgacgcc atgtaatcct gcaccttctg tcttcaggtc aactatctgc atttttttgc
    30001 cctgagtaac acagaaatgg gctgcatcat tttttactat attttctgca ccagatattc
    30061 tgcccctggc taaagaagct tcggcttcgg tgtagtattg attatcgagt ttacgctgaa
    30121 tattactttt atatgcaaga ccaaatttac cgatacttgt ctc
//
LOCUS       DAPHDW010000045        30142 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.045, whole genome shotgun sequence.
ACCESSION   DAPHDW010000045 DAPHDW010000000
VERSION     DAPHDW010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 30142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.045"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(29..346)
                     /gene="metJ"
                     /locus_tag="RH057_002006"
     CDS             complement(29..346)
                     /gene="metJ"
                     /locus_tag="RH057_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HDV3128375.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            623..1783
                     /gene="metB"
                     /locus_tag="RH057_002007"
     CDS             623..1783
                     /gene="metB"
                     /locus_tag="RH057_002007"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HDV3128376.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLRRFLSGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            1786..4218
                     /gene="metL"
                     /locus_tag="RH057_002008"
     CDS             1786..4218
                     /gene="metL"
                     /locus_tag="RH057_002008"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HDV3128377.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEYSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPGKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDHPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            4567..5457
                     /gene="metF"
                     /locus_tag="RH057_002009"
     CDS             4567..5457
                     /gene="metF"
                     /locus_tag="RH057_002009"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HDV3128378.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            5786..7966
                     /gene="katG"
                     /locus_tag="RH057_002010"
     CDS             5786..7966
                     /gene="katG"
                     /locus_tag="RH057_002010"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HDV3128379.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPTQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPAEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            8060..8965
                     /gene="yijE"
                     /locus_tag="RH057_002011"
     CDS             8060..8965
                     /gene="yijE"
                     /locus_tag="RH057_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HDV3128380.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYTRHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGAVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(8992..9609)
                     /locus_tag="RH057_002012"
     CDS             complement(8992..9609)
                     /locus_tag="RH057_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
                     /protein_id="HDV3128381.1"
                     /translation="MKASLALLSLLTAFTSHSLKSPAVPPTVVQIQANTNLAIADGAR
                     QQIGSTLFYDPAYMQLTYPGGDVPQERGVCSDVVIRALRSQKVDLQKLVHEDMAKNFA
                     EYPQKWQLKRPDSNIDHRRVPNLETWFTRHDKTRPISKNPSDYQAGDIVSWRLDNGLA
                     HIGVVSDGFARDGTPLVIHNIGAGAQEEDVLFSWRMVGHYRYFVK"
     gene            complement(9884..10987)
                     /gene="gldA"
                     /locus_tag="RH057_002013"
     CDS             complement(9884..10987)
                     /gene="gldA"
                     /locus_tag="RH057_002013"
                     /EC_number="1.1.1.6"
                     /EC_number="1.1.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
                     /protein_id="HDV3128382.1"
                     /translation="MDRIIQSPGKYIQGADVINRLGEYLKPLAERWLVVGDKFVLGFA
                     QSTVEKSFKDAGLVVEIAPFGGECSQNEIDRLRGIAETAQCGAILGIGGGKTLDTAKA
                     LAHFMGVPVALAPTIASTDAPCSALSVIYTDEGEFDRYLLLPNNPNMVIVDTKIVAGA
                     PARLLAAGIGDALATWFEARACSRSGATTMAGGKCTQAALALAELCYNTLLEEGEKAM
                     LAAEQHVVTPALERVIEANTYLSGVGFESGGLAAAHAVHNGLTAIPDAHHYYHGEKVA
                     FGTLTQLVLENAPVEEIETVAALSHAVGLPITLAQLDIKEDVPAKMRIVAEAACAEGE
                     TIHNMPGGATPDQVYAALLVADQYGQRFLQEWE"
     gene            complement(10998..11660)
                     /gene="fsa"
                     /locus_tag="RH057_002014"
     CDS             complement(10998..11660)
                     /gene="fsa"
                     /locus_tag="RH057_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV3128383.1"
                     /translation="MELYLDTANVAEVERLARIFPIAGVTTNPSIIAASKESIWEVLP
                     RLQKAIGDEGILFAQTMSRDAQGMVEEAKRLRDAIPGIVVKIPVTSEGLAAIKILKKE
                     GITTLGTAVYSAAQGLLAALAGAKYVAPYVNRVDAQGGDGIRTVHELQALLEMHAPES
                     MVLAASFKTPRQALDCLLAGCESITLPLDVAQQMLNTPAVESAIEKFEYDWNAAFGTT
                     HL"
     gene            complement(11672..14173)
                     /gene="ptsP"
                     /locus_tag="RH057_002015"
     CDS             complement(11672..14173)
                     /gene="ptsP"
                     /locus_tag="RH057_002015"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV3128384.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLAGDNCQLLISGADEQEAHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALGNLPAAKGVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLREHLLAGVSAGLSCAEAIVTS
                     ANHFCEEFARSSSSYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQPAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRVWLTQQARTADGIRIEIAANIAHSVE
                     AQAAFGNGAEGVGLFRTEMLYMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGG
                     DKPVDYLNIPAEANPFLGYRAVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSM
                     EEILWVKEKLAEAKQQLRNEHIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSN
                     DLTQYLLAVDRDNAKVTRHYNSLNPAFLRALDYAVQAVHRQGKWIGLCGELGAKGSVL
                     PLLVGLGLDELSMSAPSIPAAKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFPM
                     TQQDAPLVTAECITLESDWRSKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFST
                     GLGFSFAIPHSKSEHIEQSTISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIF
                     SRLARRIMHEEFRNALVNAASADAIASLLQHELEL"
     gene            14482..15561
                     /locus_tag="RH057_002016"
     CDS             14482..15561
                     /locus_tag="RH057_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HDV3128385.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIVGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15576..15896
                     /locus_tag="RH057_002017"
     CDS             15576..15896
                     /locus_tag="RH057_002017"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV3128386.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            15947..18244
                     /locus_tag="RH057_002018"
     CDS             15947..18244
                     /locus_tag="RH057_002018"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HDV3128387.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATSTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQTSLTQGEDAAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDDVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLYEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRAFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            18210..19088
                     /locus_tag="RH057_002019"
     CDS             18210..19088
                     /locus_tag="RH057_002019"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HDV3128388.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLIPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            19090..19431
                     /locus_tag="RH057_002020"
     CDS             19090..19431
                     /locus_tag="RH057_002020"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HDV3128389.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(19418..20269)
                     /locus_tag="RH057_002021"
     CDS             complement(19418..20269)
                     /locus_tag="RH057_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3128390.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPAPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVPAGGWNFPQWQAPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTERSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(20484..22217)
                     /locus_tag="RH057_002022"
     CDS             complement(20484..22217)
                     /locus_tag="RH057_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HDV3128391.1"
                     /translation="MHSTEVQAKPLFSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWAASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            complement(22400..25051)
                     /gene="ppc"
                     /locus_tag="RH057_002023"
     CDS             complement(22400..25051)
                     /gene="ppc"
                     /locus_tag="RH057_002023"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HDV3128392.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            complement(25403..26554)
                     /gene="argE"
                     /locus_tag="RH057_002024"
     CDS             complement(25403..26554)
                     /gene="argE"
                     /locus_tag="RH057_002024"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HDV3128393.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASCGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGVNAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPLPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELITQVIHHFCWH"
     gene            26708..27712
                     /gene="argC"
                     /locus_tag="RH057_002025"
     CDS             26708..27712
                     /gene="argC"
                     /locus_tag="RH057_002025"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HDV3128394.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVDLPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQVLQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            27720..28496
                     /gene="argB"
                     /locus_tag="RH057_002026"
     CDS             27720..28496
                     /gene="argB"
                     /locus_tag="RH057_002026"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HDV3128395.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRHAEQLPALFNGMPMGTRILA"
     gene            28557..29930
                     /gene="argH"
                     /locus_tag="RH057_002027"
     CDS             28557..29930
                     /gene="argH"
                     /locus_tag="RH057_002027"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HDV3128396.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLDELQKFSPVIDEDVYPILSLQSCLDKRAAKGGVSPQQVAQAIA
                     FAQARLE"
BASE COUNT         6784 a         7694 c         8429 g         7235 t
ORIGIN      
        1 gttccactcc gcgccgctct tttccgcttt agtattccca cgtctccggg ttaatcccca
       61 tctcacgcat gatctctttt gccgcttccg ggatttcgtc gctgcgctct ttacgcagat
      121 cggcatcatc cggcaaaggt tgcccggtaa aggcatgcag aaacgcttcg cacagcagct
      181 cgctgttggt agcgtgacgc aggttgttca cctgacgacg cgtgcgttca tcggtgagga
      241 tttttaacac cttaagagga atggaaaccg taatcttttt gacttgttca ctcttcttgc
      301 cgtgctcagc gtatgggctg atatattcgc cgctccattc agccatgaga tacttaatcc
      361 tcttcgtcaa taaattgaga ccagaccaca cagttaatgt gggcactggc cgtaaacccg
      421 catagtttac cgtacaggcg ctaccgttac atcgggtaat ccacctgctg gcgtgataat
      481 gcatataatt ttaacggcta tttgggattt gctcaatcta tacgcaaaga agtttagatg
      541 tccagatgta ttgacgtcca ttaccacaat gtttactctg gtgcctgaca tttcaccaat
      601 aaagcccagg gaacatcatc acatgacgcg taaacaggcc accatcgcag tgcgtagcgg
      661 gttaaatgac gacgaacagt atggttgcgt tgtcccaccc atccatcttt ccagtaccta
      721 taactttacc ggatttaatg aaccgcgcgc gcatgattac tcgcgtcgtg gcaacccaac
      781 gcgcgatgtg gttcagcgag cactggcaga actggaaggc ggtgctggtg cagtacttac
      841 taataccggc atgtccgcga ttcacctggt aacgaccgtc tttttgaaac ctggcgatct
      901 gctggtagcg ccgcacgact gctacggcgg tagctatcgc ctgttcgaca gcctggcgaa
      961 acgtggttgc tatcgcgtgt tgtttgttga tcaaggcgat gaacaggcat tacgggcagc
     1021 gctggcagaa aaacccaaac tggtactggt agaaagccca agtaatccat tgttacgcgt
     1081 cgtggatatt gcgaaaatct gccatctggc aagggaagtc ggggcggtga gcgtggtgga
     1141 taacaccttc ttaagcccgg cattacaaaa tccgctggca ttaggtgccg atctggtgtt
     1201 gcattcatgc acgaaatatc tgaacggtca ctcagacgtg gtggccggcg tggtgattgc
     1261 taaagacccg gacgttgtca ctgaacttgc ctggtgggca aacaatattg gcgtgacggg
     1321 cggcgcgttt gacagctatc tgctgctacg cgggttgcga acgctggtgc cgcgcatgga
     1381 gttggcccag cgtaacgcgc aggcgattgt gaaatatctg caaacccagc cgttggtgaa
     1441 aaaactgtat cacccgtcgc tgccggaaaa tcaggggcat gaaattgccg cgcgtcagca
     1501 aaaaggcttt ggcgcaatgt tgagttttga actggatggc gatgagcaga cgctgcgtcg
     1561 tttcctgagc gggctgtcgt tgtttacgct ggcggaatca ttagggggag tggaaagttt
     1621 aatctctcac gccgcaacca tgacacatgc aggcatggca ccagaagcgc gtgctgccgc
     1681 cgggatctcc gagacgctgc tgcgtatctc caccggtatt gaagatggcg aagatttaat
     1741 tgccgacctg gaaaatggct tccgggctgc aaacaagggg taaaaatgag tgtgattgcg
     1801 caggcagggg cgaaaggtcg tcagctgcat aaatttggtg gcagtagtct ggctgatgtg
     1861 aagtgttatt tgcgtgtcgc gggcattatg gcggagtact ctcagcctga cgatatgatg
     1921 gtggtttccg ccgccggtag caccactaac cagttgatta actggttgaa actaagccag
     1981 accgatcgtc tctctgcgca tcaggttcaa caaacgctgc gtcgctatca gtgcgatctg
     2041 attagcggtc tgctacccgc tgaagaagcc gatagcctca ttagcgcttt tgtcagcgac
     2101 cttgagcgcc tggcggcgct gctcgacagc ggtattaacg acgcagtgta tgcggaagtg
     2161 gtggggcacg gtgaagtgtg gtcggcacgc ctgatgtctg cggtacttaa tcaacaaggg
     2221 ctgccagcgg cctggcttga tgcccgcgag tttttacgcg ctgaacgcgc cgcacaaccg
     2281 caggttgatg aagggctttc ttacccgttg ctgcaacagc tgctggtgca acatccgggc
     2341 aaacgtctgg tggtgaccgg atttatcagc cgcaacaacg ccggtgaaac ggtgctgctg
     2401 gggcgtaacg gttccgacta ttccgcgaca caaatcggtg cgctggcggg tgtttctcgc
     2461 gtaaccatct ggagcgacgt cgccggggta tacagtgccg acccgcgtaa agtgaaagat
     2521 gcctgcctgc tgccgttgct gcgtctggat gaggccagcg aactggcgcg cctggcggct
     2581 cccgttcttc acgcccgtac tttacagccg gtttctggca gcgaaatcga cctgcaactg
     2641 cgctgtagct acacgccgga tcaaggttcc acgcgcattg aacgcgtgct ggcctccggt
     2701 actggtgcgc gtattgtcac cagccacgat gatgtctgtt tgattgagtt tcaggtgccc
     2761 gccagtcagg atttcaaact ggcgcataaa gagatcgacc aaatcctgaa acgcgcgcag
     2821 gtacgcccgc tggcggttgg cgtacataac gatcgccagt tgctgcaatt ttgctacacc
     2881 tcagaagtgg ccgacagtgc gctgaaaatc ctcgacgaag cgggattacc tggcgaactg
     2941 cgcctgcgtc aggggctggc gctggtggcg atggtcggtg caggcgtcac ccgtaacccg
     3001 ctgcattgcc accgcttctg gcagcaactg aaaggccagc cggtcgaatt tacctggcag
     3061 tccgatgacg gcatcagcct ggtggcagta ctgcgcaccg gcccgaccga aagcctgatt
     3121 caggggctgc atcagtccgt cttccgcgca gaaaaacgca tcggcctggt attgttcggt
     3181 aagggcaata tcggttcccg ttggctggaa ctgttcgccc gtgagcagag cacgctttcg
     3241 gcacgtaccg gctttgagtt tgtgctggca ggtgtggtgg acagccgccg cagcctgttg
     3301 agctatgacg ggctggacgc cagccgcgcg ttagccttct tcaacgatga agcggttgag
     3361 caggatgaag agtcgttgtt cctgtggatg cgcgcccatc cgtatgatga tttagtggtg
     3421 ctggacgtta ccgccagcca gcagcttgct gatcagtatc ttgatttcgc cagccacggt
     3481 ttccacgtta tcagcgccaa caaactggcg ggagccagcg acagcaataa atatcgccag
     3541 atccacgacg ccttcgaaaa aaccgggcgt cactggctgt acaatgccac cgtcggtgcg
     3601 ggcttgccga tcaaccacac cgtgcgcgat ctgatcgaca gcggcgatac tattttgtcg
     3661 atcagcggga tcttctccgg cacgctctcc tggctgttcc tgcaattcga cggtagcgtg
     3721 ccgtttaccg agctggtgga tcaggcgtgg cagcagggct taaccgagcc tgatccgcgt
     3781 gacgacctct ccggcaaaga cgtaatgcgc aagctggtaa ttctggcgcg tgaagcgggt
     3841 tacaacattg aaccggacca ggtacgtgtg gaatcgctgg tgcctgctca ttgcgaaggc
     3901 ggcagcatcg accatttctt tgaaaatggc gatgaactta acgagcagat ggtgcaacgg
     3961 ctggaagcgg cccgcgaaat ggggctggtg ctgcgctacg tggcgcgttt cgatgccaat
     4021 ggcaaagcgc gtgtgggcgt ggaagcggtg cgtgaagacc atccgctggc atcactgctg
     4081 ccgtgcgata acgtctttgc catcgaaagc cgctggtatc gcgacaaccc gctggtgatc
     4141 cgtgggcctg gcgcggggcg cgacgtcacc gccggggcga ttcagtcgga tatcaaccgg
     4201 ctggcacagt tgttgtaatt tcagaaattt aataatgccc ggtactcatg ttttcgggtc
     4261 tatggtttct aatgaaatat attgaattat cataggatta ggccggatga agcgtttacg
     4321 acgaatccgg caagaagcaa taagtacatg gttagtttat atttgcagtc cggtttgctt
     4381 tgcataccgg attttctttt tcttaccatc ctgaagtttt ttcatcttcc ctgatttttc
     4441 ctcaccatca ttggtcattt ttcggttgac gcccttcggc ttttccttca tctttacatc
     4501 tggacgtcta aatggataga tgtgcacaac acaacatata actacaagcg attgatgagg
     4561 taaggtatga gcttttttca cgccagccag cgggatgccc tgaatcagag cctggcagaa
     4621 gtccaggggc agattaacgt ttcgttcgag tttttcccgc cgcgtaccag tgaaatggag
     4681 cagaccctgt ggaactccat cgatcgcctt agcagcctga aaccgaagtt tgtatcggtg
     4741 acctatggcg cgaactccgg cgagcgcgac cgtacgcaca gcattattaa aggcattaaa
     4801 gatcgcactg gtctggaagc ggcaccgcat cttacttgca ttgatgcgac gcccgacgag
     4861 ttgcgcacca ttgcacgcga ctactggaat aacggtattc gtcatatcgt ggcgctgcgt
     4921 ggcgatctgc cgccgggaag tggtaagcca gaaatgtatg cttctgacct ggtgacgcta
     4981 ttaaaagaag tggcagattt cgatatctcc gtggcggcgt atccggaagt tcacccggaa
     5041 gcaaaaagcg ctcaggcgga tttgcttaat ctgaaacgca aagtcgatgc cggagccaac
     5101 cgcgcgatta ctcagttctt cttcgatgtc gaaagctacc tgcgttttcg tgaccgctgt
     5161 gtatcggcgg gcattgatgt ggaaattatt ccgggaattt tgccggtatc taactttaaa
     5221 caggcgaaga aatttgccga tatgaccaac gtgcgtattc cggcgtggat ggcgcaaatg
     5281 ttcgacggcc tggatgatga tgccgaaacc cgcaaactgg tgggcgcgaa tattgccatg
     5341 gatatggtga agattttaag ccgtgaagga gtgaaagatt tccacttcta tacgcttaac
     5401 cgcgctgaaa tgagttacgc gatttgccat acgctgggtg ttcgacctgg tttataaata
     5461 gtgtggcttt tgtgaaaatc acactgtgat cacaaatttt aaacagagca caaaatgctg
     5521 cctcgaaatg agggcgtaaa aataaggttg tcagctttgt tttcgccctc cttccttgaa
     5581 gaataggaat ctaaaaccct tttttataaa gcagttgtcc gaattcggac ataatcaaaa
     5641 aagcttaatt acgatcaatt tgatctacat ctctttaacc aacaatatgt aagatctcaa
     5701 ctatcgcatc cgtggattaa ttcaattata acttctctct aacgctgtgt atcgtaacgg
     5761 taacactgta gaggggagca cattgatgag cacgtcagac gatatccata acacgacagc
     5821 cactggcaaa tgcccgttcc atcagggcgg tcacgaccag agcgcggggg cgggcacaac
     5881 cactcgcgac tggtggccaa atcaactccg tgttgacctg ttaaaccaac attctaatcg
     5941 ttctaacccg ctgggtgagg actttgacta ccgcaaagaa ttcagcaaat tagattacta
     6001 cggcctgaaa aaagatctga aagccctgct gacagaatct cagccgtggt ggccagccga
     6061 ctggggcagc tacgccggtc tgtttattcg tatggcctgg cacggcgcag gtacttaccg
     6121 ttcaatcgat ggacgcggcg gcgcgggtcg tggtcagcaa cgttttgcac cgctgaactc
     6181 ctggccggat aacgtaagcc tcgataaagc gcgtcgcctg ttgtggccaa tcaaacagaa
     6241 atatggtcag aaaatctcct gggccgacct gtttatcctc gcaggtaacg tggcgctgga
     6301 aaactccggc ttccgtacct tcggttttgg tgccggtcgt gaagacgtct gggaaccgga
     6361 tctggatgtt aactggggtg atgaaaaagc ctggctgact caccgtcatc cggaagcgct
     6421 ggcgaaagca ccgctgggtg caaccgagat gggcctgatt tacgttaacc cggaaggccc
     6481 ggatcacagc ggcgaaccgc tttctgcggc agcagctatc cgcgcgacct tcggcaacat
     6541 gggcatgaac gacgaagaaa ccgtggcgct gattgcgggt ggtcatacgc tgggtaaaac
     6601 ccacggtgcc ggtccgacat caaacgtagg tcctgatcca gaagctgcac cgattgaaga
     6661 acaaggttta ggctgggcga gcacttacgg cagcggcgtt ggcgcagatg ccattacctc
     6721 tggtctggaa gtagtctgga cccagacgcc gacccagtgg agcaactatt tcttcgagaa
     6781 tctgttcaag tatgagtggg tacagacccg cagtccggct ggagcaatcc agttcgaagc
     6841 ggtagacgca ccggaaatta tcccggatcc gttcgatccg tcgaagaaac gtaaaccgac
     6901 aatgctggtg accgacctga cgctgcgttt tgatcctgag ttcgagaaga tctctcgtcg
     6961 tttcctcaac gatccgcagg cgttcaacga agcctttgcc cgtgcctggt tcaaactgac
     7021 gcacagggat atggggccga aatctcgcta catcgggccg gaagtgccga aagaagatct
     7081 gatctggcaa gatccgctgc cgcagccgat ctacaacccg gccgagcagg acattatcga
     7141 tctgaaattc gcgattgcgg attctggtct gtctgttagt gagctggtat cggtggcctg
     7201 ggcttctgct tctaccttcc gtggtggcga caaacgcggt ggtgccaacg gtgcgcgtct
     7261 ggcattaatg ccgcagcgcg actgggatgt gaacgccgca gccgttcgtg ctctgcctgt
     7321 tctggagaaa atccagaaag agtctggtaa agcctcgctg gcggatatca tcgtgctggc
     7381 tggtgtggtt ggtgttgaga aagccgcaag cgccgcaggt ttgagcattc atgtaccgtt
     7441 tgcgccgggc cgcgttgatg cgcgtcagga tcagactgac attgagatgt tcgagctgct
     7501 ggagccaatt gctgacggtt tccgtaacta tcgcgctcgt ctggatgttt ccaccaccga
     7561 gtcactgctg atcgacaaag cccagcaact gaccctgacc gcgccggaaa tgaccgcgct
     7621 ggtgggcggg atgcgcgtac tgggtgccaa cttcgatggc agcaaaaacg gcgtcttcac
     7681 tgaccgcgtt ggcgtattga gcaatgactt cttcgtgaac ttgctggata tgcgttacga
     7741 gtggaaagcg accgacgaat cgaaagagct gtttgaaggc cgtgaccgcg aaaccggcga
     7801 agtgaaatac actgccagcc gtgcggatct ggtatttggt tctaactctg tcctgcgtgc
     7861 ggtggctgaa gtttacgcca gcagcgatgc tcacgagaag tttgttaaag acttcgtggc
     7921 ggcatgggtg aaagtgatga acctcgaccg tttcgacctg ctgtaatctg accttcttca
     7981 gcgactgcct ttcaggcagt cgctgaagtt tctttaccgg cgtatagtct ccacaggaaa
     8041 actacacact ggatctctca tgtctgccgc aggaaagagc aacccactgg caatcagtgg
     8101 cctggttgtg ctcacactta tctggagtta tagctggatt ttcatgaagc aagtcaccag
     8161 ctacatcggt gccttcgact ttaccgcctt acgctgcatt ttcggcgctc tcgttttatt
     8221 catcgtcctt ttattacgtg gtcgcggaat gcgcccgaca ccgtttaaat acaccttagc
     8281 cattgccctg ttacaaacct gcgggatggt tggcctggcg cagtgggcgc tggtcagcgg
     8341 cggagcgggg aaggtggcga tcctaagcta taccatgccg ttctgggtgg tgattttcgc
     8401 cgcgttgttt ctcggtgaac gcctgcgacg tgggcaatat ttcgcgatac tgattgccgc
     8461 tttcggctta tttttggtgt tgcagccgtg gcaactcgat ttctcttcaa tgaaaagtgc
     8521 catgctggca atcctctccg gcgtcagttg gggggcgagc gcgattgttg ctaaacgcct
     8581 atacacccgt catccgcgcg tggatttatt atcgttaaca tcctggcaga tgctctatgc
     8641 ggcgctggtg atgagtgtgg tcgctttact ggtgccgcaa cgtgaaattg actggcagcc
     8701 caccgtgttc tgggcgctgg cctacagtgc gattctggcg acggcgctgg cgtggagctt
     8761 atggttgttt gtattgaaaa acttgcctgc cagtattgcc agcttaagca cgctggccgt
     8821 tcccgtttgc ggcgtactct tttcctggtg gctgctcggt gagaatccgg gggccgttga
     8881 aggtagcggt attgtgctga ttgtgctggc actggcgctg gtgagccgta agaaaaaaga
     8941 agctgtcagt gtaaaaagca tctgaatttt ttcttcatgt ggggcgatcg cttatttaac
     9001 aaaataacga taatgcccca ccatccgcca gctaaacagc acatcttctt cctgcgcgcc
     9061 tgcgccaatg ttatgtatca ccagcggcgt accgtcgcgg gcgaagccat ctgacaccac
     9121 cccaatatgt gccagtccgt tatccagtcg ccaggagaca atatcgcccg cttgatagtc
     9181 actggggttc ttgctgatgg ggcgtgtttt atcgtggcgg gtaaaccagg tttccagatt
     9241 aggcacccga cggtgatcga tgttgctgtc cgggcgcttt aactgccatt tttgcgggta
     9301 ctcggcaaaa ttcttcgcca tatcttcgtg caccagtttc tgcaaatcga ccttctggct
     9361 gcgcaatgca cggatcacca catcggaaca taccccgcgt tcttgcggga catcaccgcc
     9421 aggataggta agctgcatat acgccggatc gtaaaatagc gtgctgccga tttgctgtct
     9481 ggcaccgtct gcgatggcaa ggttggtatt ggcctggatt tgtaccacgg ttggtggaac
     9541 ggcgggagat tttaaggagt ggctggtaaa tgccgttagc aggctgagca gcgccagtga
     9601 agctttcatc tggttcgtcc gtgaaaataa gagtcacaga ttcgaccttc cgggctaacg
     9661 attgttctgt ggagaaaatg tccagaagcg gataaaaaac tcctgaacaa cgtgggagtt
     9721 tcagattact ggcacagagt gccggatgcg gagtgaacgc cttatccggc ctacaaaagc
     9781 acgcatattc aatatattgc agagatttcg taggcttgat aaacgtagcg catcaggcaa
     9841 ttttgcgttc aaactcccgg acaagccggg agtttggagt aggttattcc cactcttgca
     9901 ggaaacgctg accgtactgg tcggctacca gcagagcggc gtaaacctga tctggcgtcg
     9961 cgccgccagg catgttgtga atggtttcac cttctgcaca tgccgcttct gccacaattc
    10021 gcattttcgc cgggacatct tctttaatat ccagttgagc gagagttatt ggcaaaccta
    10081 ccgcatggct aagtgcagct acggtttcga tttcctcaac cggtgcgttt tccagaacca
    10141 gctgcgtcag cgtaccaaat gccacttttt caccgtgata atagtgatgc gcgtccggga
    10201 tagcggtcag gccgttatgc accgcgtgag ccgccgccag cccgccgctt tcaaaaccaa
    10261 cgccgctcaa ataggtgttc gcttcaatca cgcgctccag cgccggggtt actacatgct
    10321 gttcggcggc aagcatcgct ttttcgcctt cttccagcag ggtgttgtag cacagttcag
    10381 ccagtgccag cgccgcttgg gtgcacttgc cgcccgccat ggtggtcgcg ccgctacgag
    10441 agcaagcacg cgcttcaaac caggttgcca gcgcatcgcc gatacccgct gctaacagac
    10501 gtgcaggtgc gcccgcgacg attttggtgt cgacaatgac catattcggg ttatttggca
    10561 acagcagata gcggtcaaac tcaccctcat cggtgtagat aacagacaat gcgctgcacg
    10621 gtgcatcggt agaggcgata gtcggtgcga gcgctaccgg aacacccatg aaatgtgcca
    10681 gtgctttggc agtatcgagg gtttttccgc caccgatacc gagaattgcg ccacactgcg
    10741 cagtctccgc gatgccacgc agacggtcga tctcattttg cgaacattca ccgccaaacg
    10801 gcgcaatttc tactaccagt ccagcatctt taaagctttt ctcgacagtg gattgagcaa
    10861 aacctaaaac aaatttgtca cccaccacta accagcgttc tgccagcggc ttcaggtatt
    10921 cgcccagacg attaatcaca tcagcgccct ggatgtattt acccggtgat tgaataatgc
    10981 ggtccataat tgctccttta gagatgagta gtgccaaatg cggcattcca gtcgtattca
    11041 aacttctcta tagctgactc taccgcaggc gtattgagca tttgttgcgc tacatctaaa
    11101 ggcagggtga tggactcaca tcctgccagc aaacaatcca gtgcctgacg cggcgtttta
    11161 aagctggctg caagcaccat gctttctggc gcgtgcattt ctaacagcgc ttgcagctca
    11221 tgaaccgtac gaataccgtc tccgccctgg gcgtctacgc ggttaacata cggagcaacg
    11281 tattttgctc ctgccagtgc ggctaataac ccttgtgcgg cgctatatac agcggtgcca
    11341 agtgtagtaa taccctcttt tttcagtatt ttaattgctg ccagaccttc ggaagtcacc
    11401 gggattttca ccacaatgcc cggaatggcg tcgcgcagac gcttcgcttc ttccaccatc
    11461 ccctgcgcgt cgcggctcat ggtctgagca aacagaatgc cctcatcacc aatcgctttt
    11521 tgcagacgcg gcagcacttc ccagatggac tccttgctgg cagcgataat gctcgggtta
    11581 gttgtcaccc cggcaatcgg gaatatgcgt gccagacgtt cgacttctgc gacgttagcg
    11641 gtgtccagat acagttccat gatgtttcct tttacagttc cagttcatgt tgcagcaggc
    11701 tggcgatagc gtcggcagag gcggcgttaa ccagcgcgtt acggaattct tcgtgcatga
    11761 tgcggcgagc gaggcgcgag aaaatgcgca tgtgctgatc gcccgcagcg tgtttgttca
    11821 gggttaacat aatgatgaat tgcgcttcat catcgcccca gcgcaccggc gcttgcagac
    11881 gcgccacgct gatggtggat tgctcaatgt gttctgattt gctgtgtgga atggcaaaac
    11941 taaagcccag accggtagag aaaacggcct cgcgcgccca taagtcggct tccagtttac
    12001 gcggatagcg gcagcggccc gccagcagca ggttatcggt catgcctttg agcacttctt
    12061 ctttgctgcg ccagtcactt tccagcgtga tgcactcggc ggtgaccagc ggtgcgtcct
    12121 gttgggtcat ggggaattgc gccagcaggt gttccacttc cagcgaagta cggcaggcca
    12181 ttgcctggtt gagcaacttg cggcactcac ggctatcaag ttgcgccatc cgcgctttcg
    12241 ccgccggaat tgatggtgcg ctcatgctga gttcatccag ccctaagccg accagcaacg
    12301 gcagcacgga acctttcgct cccagctcac cgcacagacc aatccatttg ccctggcgat
    12361 gcaccgcctg cacggcgtaa tcgagcgccc gcaagaatgc cggattcagg ctgttgtagt
    12421 gacgagtaac cttagcgtta tcgcgatcca ctgccagcag atattgcgtc aggtcattac
    12481 taccaatact aaagaaatca atctcttcgc agcattgatc gatgatgaac atcaccgacg
    12541 gtacttccag cataatgcca agctggatct tctcatcaaa cggaatgtgt tcgttacgca
    12601 gttgctgttt ggcttccgcc agtttttctt tcacccataa gatctcttcc attgaggaga
    12661 tcatcgggat catgattttc aggctgccgt gagcggaggc gcgcaggatc gaccgtagtt
    12721 gtgtggtgaa cagcgaggcg tattcttcat aaatacgcac agcgcgataa ccgaggaacg
    12781 ggttagcttc tgccggaatg ttcagataat caacgggttt gtcgccgcca atgtccatag
    12841 tgcgcacaat aatgctgcgt ccgttggcag actccagcgc ctgacaaaaa atgttgtaca
    12901 gctcgctttc gcccggtgca ctggtgcgat ccatatagag catttcagtg cggaacaaac
    12961 caacgccttc cgcaccattg ccgaatgcgg cctgcgcttc cacggagtga gcgatgttag
    13021 cggcaatttc aatgcggata ccgtcagcgg tacgggcttg ttgggtcagc cagacacgct
    13081 gttgctcacg cagggcgtcc tgtacgcggg cttcttgctg ataataacga gctacggctt
    13141 ctcctggctc aaccacaatc gccccagcgt tgccgtcgat ataaatcgtt tgatgctgcc
    13201 acggagtaag ggcatcaata tctacaccaa ccagtgtcgg aatgttgaac gaacgggcaa
    13261 gaattacggt atgtgaggtg gtgccgccac ttttcagtaa cagacctttg aggtgatttt
    13321 tatccagttc gaggaactgg ctgggagtca gttcatcagc catacaaatg gcgggctgcg
    13381 tcagtttgcc cggtgccggg aagcgttgct caccgtagat ttgctggagt aactggaagc
    13441 agacgtcgcg tacgtccagg gcacgttctt gcaggtagct gctgctggaa cgggcgaact
    13501 cttcgcaaaa gtgattcgca ctggtgacaa tcgcttcggc gcagcttaat ccggcgctga
    13561 cgcctgccag taaatgttcg cgcagggaag tatcgccagc cagggatcgg tgcgcttcca
    13621 gaatcgcgct ggtggcaccg tcgctatcca gcagacgaaa ctcaatgttt ttcagtacca
    13681 gcgtcaggcc gttttccagt gcggattgct cggcgtcaac gcctttggct gcgggaagat
    13741 tacccagcgc attgagatct aaagaagaga tcggcgtcag aatgccgcct gcactaccgc
    13801 tgcacacggt gcgggcgcgg ataatttgcg gattcagatt ggtcagtgaa accggcagtg
    13861 gttccagttc gtcagattta acttccgcca gcggcgcatc gcagtggggg aattcatcgc
    13921 gcagccattg gcttaaacgc tggtgcgctt cctgttcgtc ggccccggaa atcagtaact
    13981 ggcagttatc gcccgccagc gtatcggtgc caatcagcgc aagggcgctt ttggcgttgc
    14041 ccttgcggtc agtgcgcagg ttatgccact caatttgtga tgaaaaagta ttacacagcg
    14101 tttcaacgtg gcttgccgga cgtgcatgta cgccgttagg tagctcacaa ataaattcca
    14161 caatcagggc cattgcctct ctcccggtac ggttttctgt ttacaggata agagcgcacg
    14221 gcaacggcct gccatgtgac aaatctgcca aaagctggac aaatgtaatg taaccgtcaa
    14281 tttgcgacgc gtctcacaag acgctgtttt gcggcatgct tccggtttat cgcaagttat
    14341 gaggcggatc gcatttttgt actgatatta caaaagtcca gtaaatggcc tttttatcca
    14401 ctgtttgctc cgcctgcgat tgcctattgt ttcctccatc aaaaatatgg acatgggctt
    14461 tcgcaagccc gggagcagct tatgaatgag ttggtgcaga tcctgaaaaa tacccgtcag
    14521 catttaatga cgggcgtttc acacatgatt cccttcgtgg tatcgggcgg tattttgctg
    14581 gcggtttccg tcatgttgta tggcaaaggc gcagtgccgg atgccgtagc cgatccaaat
    14641 ctgaaaaaac tgtttgatat cggcgttgcg ggcttgacgc tgatggtgcc tttcctcgca
    14701 gcgtacatcg gttattccat tgcagagcgt tctgcgctgg ctccgtgcgc tatcggtgcc
    14761 tgggttggta acagctttgg tgcgggcttc tttggtgcac tgatcgccgg gattatcggc
    14821 ggcatcgtgg tgcattacct gaagaaaatt ccggtgcata aagttctgcg ctcggtgatg
    14881 cctatcttca ttattcctat cgtcggcaca ctgattaccg caggcatcat gatgtggggc
    14941 ttgggcgagc ctgtaggggc gttgaccaac agcctgactc agtggcttca ggggatgcag
    15001 cagggcagca ttgttatgct ggcggtgatc atgggtctga tgctggcgtt cgatatgggc
    15061 ggtccggtta acaaagtggc ctatgccttc atgctgattt gcgttgctca gggtgtttat
    15121 accgtggtgg ccatcgctgc cgtgggtatt tgtatcccgc cgctggggat ggggctggcg
    15181 acgctgattg gtcgtaaaaa tttctccgca gaagagcgcg aaaccggcaa agcggcactg
    15241 gtgatgggct gtgttggcgt tactgaaggg gcgattcctt tcgccgctgc cgatccgctg
    15301 cgtgtcattc cttccatcat ggtcggttca gtttgtggtg cagtaactgc ggcgctggtc
    15361 ggtgcgcagt gctatgcagg ctggggtggt ctgattgtgc tgccggtggt tgaaggcaag
    15421 ctgggttata tcgcagcagt ggctgtcgga gcagtggtga cggctgtttg tgtgaacgtg
    15481 ctgaaaagtc tggcgcgtaa aaatgggtct tcgactgatg aaaaagaaga cgacctggat
    15541 ttggattttg aaattaatta attgaggaac gagccatgac gaaaattatt gcagtaaccg
    15601 catgtccttc aggtgttgcc catacctata tggcagcaga ggcgctggaa agtgccgcga
    15661 aagcgaaagg ctgggaagtg aaagtagaaa cccaggggtc aattggtctg gaaaatgaac
    15721 tgactgcgga agatgtggcg agcgctgata tggtgattct gaccaaagat atcggcatca
    15781 agtttgaaga gcgttttgct ggcaagacca tcgtgcgcgt caacatcagc gatgcagtaa
    15841 aacgtgctga cgccattatg agcaaaattg aagctcacct ggcgcaaacc gcttaagttc
    15901 ttctcccgct cgcaagggcg ggttcgcttt cccacaggag ttcctcatga cgaatcgtat
    15961 ctctcgcctc aaaactgcac tgtttgccaa tacccgtgaa atctcgctgg agcgggcgct
    16021 gctttatacc gccagccatc ggcaaaccga aggcgaaccg gtgatattgc gccgggcgaa
    16081 ggcaacagcg tatatccttg aacatgttga aatttcgatt cgtgatgaag aactgattgc
    16141 cggtaaccgc accgtaaaac cgcgcgccgg gattatgtcg ccggaaatgg acccttactg
    16201 gctgctgaaa gagctggatc aattcccgac gcgtccgcag gaccgctttg ctatcagcga
    16261 agaagataaa cgtatctacc gcgaagagtt gttcccgtac tgggaaaaac gttcgatgaa
    16321 agatttcatc aacgggcaga tgacagatga agtaaaagcc gcgaccagca cgcagatttt
    16381 cagcatcaac cagacagata aaggccaggg gcacattatt attgattacc cacgcctgtt
    16441 gaatcacggg ctgggggagc tggtggcaca gatgcagcaa cattgtcagc aacagccgga
    16501 gaatcacttt tatcaggcag cgctgttact gctggaagcc tcgcagaaac acattttgcg
    16561 ttacgccgaa ctggcggaaa cgatggcggc aaactgcaca gatgcccagc gtcgcgaaga
    16621 gctgctgact attgcggaga tctcccgcca taacgcccaa cataagccgc agacgttctg
    16681 gcaggcgtgc cagttattct ggtacatgaa catcattctg caatacgaat ccaacgccag
    16741 ttcgctatcg ttggggcgct tcgaccagta tatgttgccg ttctatcaga catcattaac
    16801 ccagggcgaa gatgcggcgt tcctgaaaga actgctcgaa tctttatggg tgaaatgcaa
    16861 cgacatcgtg ctgttgcgct ccaccagtag cgcgcgttat ttcgccggtt tcccgaccgg
    16921 ctataccgca ctgctcggcg ggttaaccga gaacggacgt agcgcggtga acgtgctttc
    16981 gttcctttgc cttgacgcct atcaaagcgt gcaattaccg caaccgaacc tcggcgtgcg
    17041 cactaacgcc ttgatcgaca cgccgttcct gatgaaaacc gccgaaacca ttcgcctcgg
    17101 caccggtatt ccgcaaatct ttaacgatga cgtggtggtg ccagcgttcc tcaaccgtgg
    17161 cgtttcgctg gaagatgcgc gcgactattc cgtagtgggc tgtgtggaat tatctattcc
    17221 cggcagaacc tacggcttgc atgacatcgc gatgtttaat ctgctgaaag tgatggaaat
    17281 ctgcctgtat gaaaatgaag gcaatgctgc gctgacttat gaaggtttac tggagcagat
    17341 ccgcgccaag atcagccact acatcaccct gatggttgag ggcagcaata tttgtgatat
    17401 cggccatcgc gactgggcac ctgtaccgct gctctcgtct tttatcagcg attgtctgga
    17461 aaaaggccgc gatattaccg atggcggcgc gcgttataac ttctccggcg tacaggggat
    17521 cggtatcgcc aacctgagcg attctctcca tgcgttgaaa gggatggttt ttgagcaaca
    17581 gcgtttaagt tttgacgaat tgctgtcggt attaaaagcc aactttgcaa cgccagaagg
    17641 cgaaaaagtc cgtgctcgct taattaaccg cttcgagaaa tacggtaacg atatcgacga
    17701 ggtggataac atcagcgccg aactgttacg ccactactgc aaagaagtgg aaaaatacca
    17761 gaacccgcgc ggcggctact tcacgccggg atcgtatacc gtttctgctc acgtcccgtt
    17821 gggatcggtg gttggagcga cgccagacgg tcgttttgcc ggagaacagc tggcagacgg
    17881 cggcttgtca cccatgctgg gccaggacgc acaagggcca acagcggtac tgaagtcagt
    17941 cagtaagctc gataacacgc tgctgtctaa cggtacgttg ctgaacgtga aattcactcc
    18001 agcgaccctg gaaggtgaag caggattacg caaactggcc gacttcttac gggcatttac
    18061 ccagcttaag ttacaacata ttcagtttaa cgtggtgaac gccgacacgt tgcgggaagc
    18121 gcaacagcgc ccacaagatt atgccgggct ggtggtgcgc gttgccggat acagcgcctt
    18181 ctttgtcgaa ctgtcgaagg agatccagga tgacatcatc cgccggacag cgcatcagct
    18241 gtaacgttgt ggaaacgcgc cgcgatgatg tggcgcgcat tttcaacatt cagcgttatt
    18301 cactgaatga cggcgagggc attcgtacgg tggtcttttt taaaggctgt ccgcatcttt
    18361 gcccgtggtg tgctaatccg gagtcgatct ccggcaaaat ccagacagta cgcagagagg
    18421 cgaaatgtct gcactgtgcg aaatgtttgc gtgatgcgga tgaatgcccc tccggtgcgt
    18481 ttgaacggat tggtcgcgat atcagccttg acgcgctgga acgggaagtg atgaaagatg
    18541 acattttctt tcgcacgtcc ggcggcggcg tcacgctttc tggcggcgaa gtgttaatgc
    18601 aggcggagtt tgctacccgt tttttacagc ggctgcggtt gtggggcgtg tcatgcgcca
    18661 ttgaaactgc cggagacgcg ccagccagca agctattacc actggcgaaa ttgtgcgatg
    18721 aagtgctgtt cgatttaaaa attatggacg cgactcaggc gcgggatgtg gtgaagatga
    18781 acctgccacg cgtgctggag aatctgcgtt tgctggtgag tgagggtgtc aacgtgatcc
    18841 cgcgtttacc gttgatccct ggtttcacgc tcagccggga gaatatgcag caggcgctgg
    18901 atgtactgat cccgctgaaa atcaggcaga tccatctgtt accgtttcat cagtacggcg
    18961 aaccgaaata ccgcctgctg gggaaaacat ggtcgatgaa agaggtgcct gcgccgtcgt
    19021 cagccgatgt gacaacgatg cgcgaaatgg cagaacgggc cggatttcag gttaccgtgg
    19081 gaggttaaaa tggcatacct ggtggcagta accgcctgcg taagcggcgt ggcgcatact
    19141 tatatggcgg cggaacggct ggaaaagttg tgccagttag agaagtgggg cgtcagcatt
    19201 gaaactcagg gcgcgctggg aacggagaat cgtttagcgg acgaggatat ccgtcgggcg
    19261 gatgttgctc tgttgattac ggatatcgag cttgccggtg ccgagcgatt tgaacattgc
    19321 cgctatgtgc aatgcagcat ctacgcattc ctgcgtgagc cgcagcgggt aatgagcgcg
    19381 gtgcgcaaag tgctttctgc gccgcagcaa acccatctta ttctggagta gtcggttttt
    19441 ctgtcagttg gctgtggtac tgccggcgat attccgacgg cgagcgttcg gtgtttttac
    19501 gaaacagacg gcagaagtag ttgctgtcga caaagccgca ggcgtgcgcc acttctttta
    19561 ccttcaggtc atagcctttc aataacgtct tagcgtgctc cagtcgcgtg tgattcaggt
    19621 attcgttaaa accaatggcc cccgtttttt gaaacaggtg cgagagatag tttggtgaaa
    19681 tatagaatgc ctgtgcgaca gattcgcggg taagcgcgga ggcatagcgt tcgtcgatat
    19741 aatcgcgaat agcttcaaat agtgcctggc tgcgtgaggc ggtctggatt tggctgccga
    19801 gcaaatcgcg gcagtggcta agcaggctgg cgacgataag ccttgccgtt tgctgctcct
    19861 gcggctgcat ttgcatttca ttgagcgttt gtagcagaaa agaacctatg cgtgggccgc
    19921 gccgggcgac gtgttgcttc gccagatttt gatattgttt gccatcccat tgcacgacgc
    19981 tgaagccgag ttgctgtttg ccaaacagca cgctaaaggt agtagcgggg gcttgccatt
    20041 gtggaaaatt ccagccacca gcgggaacgt acagcacatc gccgggaact aacgttgctc
    20101 cagcgccggt atcaacaaac tcaccttcca gcactatttc cagccgtgga aaatcgacct
    20161 gatacgcaag atcgggcgcc ggaccgttac tgctggcaaa gtaaatctgg cgcagggaca
    20221 gcgggccgtt gatcaggcgg gagagcagat agctgacgtc gtgatacatg ttattccctt
    20281 aggtatgtag acgggaaaac agtaactggc attattaaaa aaggccacct gaaaagtgtg
    20341 gcctgagggg agttcgatgt aaacattgcg gcagttgtat tgccggatgt ggcgtaaacg
    20401 ccttattcag tctaccgccg cgatctgtag gccggataag acgcgtcaag cgtcgcatcc
    20461 ggcaaatagc gcctggctga taattactga ttacctacct ggtcgccata cggcagagtg
    20521 tcgtaatcga tcagcgcgtt tttcttgtac gggttaccaa tccagcgggt agtttctttg
    20581 aactgcggat tcaccactga acgggttgga tcgtaaccgt cgtaagataa gcccgccaaa
    20641 tctgaccagg tgtggatcag ttccgccagg ctgtatttac gatcgacatc ctgtgagaaa
    20701 tcacggggat gagtcgcttg ccatttttcc gacgtccaca gcaggaacgg aatggtgtac
    20761 atgtggcgcg tcgggttgtc ttcgttacgc ccctgagttt tgtgcggcgg cgtgtcgtaa
    20821 acctcttcac cgtggtcaga gaagtaaacc aggaatccgt tcgggtctgc cgctttaaag
    20881 tctttaatca ggctggcaac cacatgatcg ttatacaggt tagcgttgtc ataatcgtta
    20941 tatgattcca gctcttctgc gcttaatcct ggcggaacat gatcggtatt gccatcaaac
    21001 ttgccctgat tttccgggta gcggtatttg tatttgatat gcgtacccag cagatgaacg
    21061 atgatcagtt tcttcggcgc agggtcattc agcacatcct ggaacggctt cagcacgttg
    21121 gtgtcgtatt cacgcgcact ctgcgtacgt tgctggttca tgtagtactg cttgtcggtc
    21181 tggcgcgaaa ataccgtcag catggtattg cgggcggtca tcgtctgctg gttggtgatc
    21241 cagaaggttt tataacccgc ctgtttcatc atgttcatca gcgacggctg cgtcagatac
    21301 agatccgggt tcttttcatt ggcaaaggtc agcgcctgtt gcaggatttc aatggtgtac
    21361 ggacgagacg taactacgtt attaaacacg gtcagattcg gatcggtttt atgcagcgca
    21421 tccagctccg gcgtggtttc acgcggataa ccgtacagac tcatgcgtcc gcgctgggtc
    21481 gactcgccaa tcaccagcac taaagtgcgc ggttcgttac ccgattcatc tttgaaatta
    21541 gccagtggcg gcaaggcatt attttcattc agtaactttg ttagcgagtt tagttgctga
    21601 cgatactgat aatagccggt caggaattgc cacggtgcgg caggctccat acgcgaggcc
    21661 aggttatcca acgttttctc aaacggcttg tttttgataa acgtattcat ggcgatcgga
    21721 tgcagaatca agccataaag cagggcaaaa gagacaacat aacgccacgg ctttggaata
    21781 tagaccgggc gcaggcgtgt ccacagcaga actgccaccg ccgtataggc cagcgcgata
    21841 agcacaattt tcaggctgaa atactggctt aaatactcgc tggcttcgtt ggtgttggtt
    21901 tcgaacatca caaacagaac gctctgcgag aactcctgcc cgtagatgac gtagtagcat
    21961 agcgccgcca gagaggccgc ccatagcacc acgccgatta ctgcggcaat aattttaatc
    22021 cgcttcggaa agaggaatac cgggatcaac cacagcgagc tgaataacag cgagtcgcga
    22081 atgccgttgg tgccactata accactgatg taaataatgg cctgtagcag agtagagaaa
    22141 aaccaaaagt agagcagtgc ccaacccagg gctttccagc taaaaagggg tttagcctgg
    22201 acttctgtgg aatgcatagt aagaacctgt cttgaaaaaa atatcgccga atgttacgac
    22261 aattccttaa ggatatctga aggtatattc agaatttgaa taaaatgcag acagaaatat
    22321 attgaaaacg agggtgttag aacagaagta tttcagaaaa ccctcgcgca aaagcacgag
    22381 ggtttgcaga agaggaagat tagccggtat tacgcatacc tgccgcaatc ccggcaatag
    22441 tgaccattaa cgcttgttcg acgcgaggat ccggttcctg gccttctttt tctgcctggc
    22501 gggagcggtg cagtaactcg gcctgcaata cgttcagcgg gtcggtgtaa atattccgta
    22561 gctgaataga ctctgcaatc cacggcagat cggccatcag atgggaatcg ttggcaatcg
    22621 ccagcaccac tttgatgtct tcttcttgca ggttgcgtaa ctctttacct aacggccaca
    22681 gtgctttgtc taccaggcgt tggtcatagt attccgccag ccacaggtct gctttggcga
    22741 agaccatctc cagcatgccg agacgcgtcg agaagaatgg ccaatcgcgg cacatggctt
    22801 ccagctcgct ctgtttgcca tcttcgacca ctttttgcag cgccgtacct gcacccagcc
    22861 aggcggggag catcaggcgg ttttgcgtcc aggcaaaaat ccacggtatg gcgcgcagcg
    22921 actcgacgcc gccggttggg cgacgtttcg ccggacgtga acccaacggc agtttgccca
    22981 attcttgttc cggcgtagcg gagcggaagt aaggcacaaa atctttgttt tcacgtacgt
    23041 agccacggta gagatcgcag gagatgactg acagttcatc cataatgcga cgccagctct
    23101 ctttcggctc aggtggtggc agcaggttgg cttccagaat cgccccggtg tacagcgaca
    23161 ggctgctgac ggtgatttct ggcagaccat atttaaagcg gatcatctcg ccctgttcag
    23221 tcacgcgcag gccacctttc aggcttcctg gcggttgtga cagcagcgcc gcatgagcag
    23281 gtgcgccgcc gcgaccaatg gaaccgccgc gaccgtggaa caacgtcagc tcaatacccg
    23341 ctttttcgca ggttttgatt aatgcatcct gtgcctgata ttgcgcccag gaagctgcca
    23401 tcactcccgc atcttttgct gagtcggaat agccaatcat caccatctgt ttgccctgaa
    23461 tcaggccacg ataccagtca atattgagca gctgggtcat gacatcgttg gcgttgttca
    23521 ggtcatcgag ggtttcaaac agcggagcaa ccggcatcgc aaacccgata cccgcttctt
    23581 tcagcagcag gtggacagcc agcacgtcgg acggcgtttt cgccatcgag atcacgtagg
    23641 cggcaatgga gccttgcggc gcttcggcaa tcacctggca ggtatcgagc acttcgcgcg
    23701 tttcggcgct tggttgccag ttgcgcggta gcagcggacg tttggagttc agttcgcgga
    23761 tcaggaacgc ctgtttgtca gcttctgacc agctctcgta gtcgccgata ccgaggtagc
    23821 gggtcagttc acccagtgct tcggtatggc gggtgctctc ctgacggata tcaatacgga
    23881 ccagcggtac gccgaaacat ttcacgcggc gcagggtgtc gagcagatcg ccgttggcga
    23941 taatacccat gccacacgcc tgaagtgact ggtagcaagc gtagagcggt tcccacagtt
    24001 cttcgttttg tgtcagcagc ccttctggtt ttggcagttc ttcgcctttc aggcgcgctt
    24061 ccagccatgc ctgtgtcgcc atcaggcgag aacgcaggtt tttcatcaga tagcgatacg
    24121 gttctgcggc accttcttcg ccaaccagcg ccagcagttc aggggtcgct tcaaccatcg
    24181 acagttcaga aaccagcacc tgaatatctt tcaggaacaa atcggtggct ttccagcggc
    24241 tgagtagcag gacgtggcgg gtgatatcgg cagtgacgtt cgggttgccg tcgcggtcgc
    24301 cgcccatcca cgaagtaaaa cggaccggaa caaattcgac gggcagtttg tagccgaggt
    24361 tctcttccag ttgttcgttc agttcgcgca ggtaatttgg tacgccttgc cacaggctgt
    24421 tttccactac ggcaaagccc catttggctt catctaccgg gcttggacgc agcttacgga
    24481 tttcatcggt atgccatgac tgggcgatta actggcgcag gcgacgcatc agctggttgt
    24541 gttcgtagtc agcgatatct ttgttatcga gctgttttaa gcaggcgttc acttccacca
    24601 ttttgtggat cagtgtacga cgggtaattt cggtagggtg agccgtgagg accagttcca
    24661 gcgacagcga ttccactgct tttttgatgg tgtcttcgct cagttccggc tggtttttca
    24721 gtttacgcag ggtgcgggcg atcacttccg ggttgctggc agcttcgcct ttcggcgaaa
    24781 tgctgtggta ttgctcggcg gtgttggcca ggttcaggaa ctgactaaac gcacgcgcaa
    24841 cgggcagcag ctcgtcgttc gacaaatttt gtaaggtggt gagcaactcc tggcggttag
    24901 catcattgcc agcgcgtgaa gatttcgaca acttacggat agtttctacg cgttcaagaa
    24961 tgtgttctcc caacgcatcc ttgatggttt ctcccagcac tttgccgagc atactgacat
    25021 tactacgcaa tgcggaatat tgttcgttca tattacccca gacaccccat cttatcgttt
    25081 gatagccctg tatccttcac gtcgcattgg cgcgaatatg ctcgggcttt gcttttcgtc
    25141 gtcttttata aagccacgta aaagcggtga cgtcaaatgc tgcgaaatcg cttcagcaaa
    25201 cgaataaata gcaggaattt acgtcattaa attcacgacg ctttaaataa gcgtaactta
    25261 tggaaatgtt aaaaaatcgc cccaagtaac accaaaggtg taggtcggat aagatgcgca
    25321 agtatcgcat ccgacattat tgcggcactg gagtttgaca acagtgccgg atgcggcgcg
    25381 agcgccttat ccggcctacg ttttaatgcc agcaaaaatg gtgaattacc tgggttatca
    25441 gttcgcgggt gggcttgata aaccgtgttt ccagatattc atcaggttga tgagcctgat
    25501 taattgagcc aggccccaac accagcgtcg ggcataacgt ttgaataaac ggcgcttcgg
    25561 tacagtaatt caccacttcg gtttttgctc cgagcaattt ctcaaccact tcaaccagtt
    25621 gatgattcgg tgggcattca tagccaggga tcggcggatg cagctcgtcg accgtcagac
    25681 gacccggcca gcgttcgctc accggagcca atgcatcgtt gagcaaacca ttaagttcat
    25741 tgagtgtcat gccaggcagc ggacgaatat ccatatgcaa ctcacagcaa gcgcaaatac
    25801 ggttagaagc gtcgccaccg tgaatatgcc cgaggttgag cgtagggtat ggcacggtaa
    25861 acgcttcgta gtgataacgt tctttcaggt tatcgcgcaa ttgcaaaata tgcccgatgg
    25921 cgtcgtgcat tagttcgata gcgttaactc cgcgtgctgg atcgctggag tgccccgact
    25981 ggccctgaat acggatggcg ttagagatat gacctttatg tgcgcgtacc ggttgtagtg
    26041 acgtcggttc gccaatgatg gcgcaatccg ggcgcagggc ggtagtttcg gcaaaataac
    26101 gcgctccggc catactggtt tcttcatcag cagtcgccag aatgtagagc ggttttttca
    26161 gtttcgtgac gtcgacatcg cgtagcgcat caaggataaa cgcaaaaaag cctttcatgt
    26221 cggcggtgcc taagccgtaa agcttgccgt catgctccgt cagtgtaaac ggatcacgcg
    26281 tccagcgacc gtcatcaaat ggcaccgtat cggtatgccc cgccagcaac aagccgccag
    26341 ccccctgtcc gcaactggcc agcatattaa atttgttgcg agttcctgga acaggctgca
    26401 cttccacatt gaagcccaaa tctttaaacc agtccgccag cagagtgatt aaatctgcat
    26461 tgctttgatc gagtgcctct tccgtggcgc ttattgaagg tgtggcaatc agagcacggt
    26521 aaatctcgat aaatggcggt aatttgtttt tcattgttga cacacctctg gtcatgatag
    26581 tatcaatatt catgcagtat ttatgaataa aaatacacta acgttgagcg taataaaacc
    26641 caccagccgt aaggtgaatg ttttacgttt aacctggcaa ccagacataa gaaggtgaat
    26701 agccccgatg ttgaatacgc tgattgtggg tgccagcggc tacgctggcg cagagctagt
    26761 gacctatgta aatcgccatc cgcatatgaa cataaccgct ttgactgttt cagcgcaaag
    26821 caatgatgcg ggaaagttaa tctccgattt gcatccgcag ctaaaaggca tcgttgatct
    26881 gccgttgcag ccgatgtcgg atatcagcga gtttagccca ggggtggacg tagtgtttct
    26941 cgccaccgcc catgaagtta gccacgattt agcgccgcag tttcttgaag cgggctgcgt
    27001 ggtgttcgac ctttccggcg cgtttcgtgt taacgacgcc accttctatg aaaaatatta
    27061 cggctttacc catcaatacc cggaactgtt ggaacaggca gcctacggtc tggcggagtg
    27121 gtgcggtaat aaattaaaag aagcgaattt gattgcggtg ccgggctgtt atccgacggc
    27181 ggcacagctg gcgctgaaac cgttgattga tgccgatctt cttgacctca atcagtggcc
    27241 ggtgatcaac gccaccagcg gcgtgagcgg tgcagggcgt aaagcggcca tttcaaacag
    27301 cttttgtgaa gttagcctgc aaccgtatgg cgtctttact catcgccatc aaccagagat
    27361 cgccacacac ctcggtgctg acgttatctt caccccacat ctgggcaatt tcccgcgcgg
    27421 cattctcgaa accattacct gccgcctgaa atcgggtgtg acccaggcgc aagtcgcgca
    27481 agtgttacag caggcgtatg cccataaacc gctggtgcgg ctgtatgaca aaggcgttcc
    27541 ggcgctgaaa aatgtcgttg ggctgccatt ttgcgatatc gggtttgccg ttcagggcga
    27601 gcatctgatt attgtggcga ccgaagacaa cttactgaaa ggcgcggcgg cacaagcggt
    27661 acagtgcgcc aatattcgtt tcggctatgc ggaaacgcag tctcttattt aagggtgcaa
    27721 tgatgaatcc attaattatc aaactgggcg gcgtactgct ggatagtgaa gaggcgctgg
    27781 aacgtctgtt tagcgcactg gtgaattatc gtgagtcaca tcagcgtccg ctggtgattg
    27841 tgcacggcgg cggttgcgtg gtggatgagc tgatgaaagg gctgaatctg ccggtgaaaa
    27901 agaaaaacgg cctgcgggtg acgcctgctg atcagataga cattatcacc ggagcactgg
    27961 cgggaacggc aaataaaacc ctgttggcat gggcgaagaa acatcagatt gcggccgtag
    28021 gtttgtttct cggtgacggc gacagcgtca aagtgaccca gcttgatgaa gagttaggtc
    28081 atgttggact ggcgcagcca ggttcgccta agcttatcaa ctccttgctg gagaacggtt
    28141 atctgccggt ggtcagctcc attggcgtaa cagacgaagg gcaactgatg aacgtcaatg
    28201 ccgaccaggc ggcaacggcg ctggcggcaa cgctgggcgc ggatctgatt ttgctctccg
    28261 acgtcagcgg cattctcgac ggcaaagggc aacgcattgc cgaaatgacc gccgcgaaag
    28321 cagaacaact gattgagcag ggcattatta ctgacggcat gatagtgaaa gtgaacgcgg
    28381 cgctggatgc ggcccgcacg ctgggccgtc cggtagatat cgcctcctgg cgtcatgcgg
    28441 agcagcttcc ggcactgttt aacggtatgc cgatgggtac gcggatttta gcttaagttt
    28501 tgttggccgg aggcgcagct ttccggcatt gaatttcaaa ataaggaaac agagttatgg
    28561 cactttgggg cgggcgtttt acccaggcag cagatcaacg gttcaaacaa ttcaacgact
    28621 cactgcgctt tgattaccgt ctggcggagc aggatattgt tggctctgtg gcctggtcca
    28681 aagccctggt cacggtaggc gtgttaaccg cagaagagca ggcgcaactg gaagaggcgc
    28741 tgaacgtgtt gctggaagat gttcgcgcca ggccacaaca aatccttgaa agcgacgccg
    28801 aagatatcca tagctgggtg gaaggcaaac tgatcgacaa agtgggccag ttaggcaaaa
    28861 agctgcatac cgggcgtagc cgtaatgatc aggtagcgac tgacctgaaa ctgtggtgca
    28921 aagataccgt tagcgagtta ctgacggcta accggcagct gcaatcggcg ctggtggaaa
    28981 ccgcacaaaa caatcaggac gcggtaatgc caggttacac tcacctgcaa cgcgcccagc
    29041 cggtgacgtt cgcgcactgg tgcctggcct atgttgagat gctggcgcgt gatgaaagcc
    29101 gtttgcagga tgcgcttaag cgtctggatg tcagcccgct aggctgtggc gcgctggcgg
    29161 gaacggccta tgaaatcgac cgtgaacagt tagcaggctg gctgggcttt gcttcggcga
    29221 cccgtaacag tctcgacagc gtttctgacc gtgaccatgt gttggaactg ctttctgctg
    29281 ccgctatcgg catggtgcat ctgtcgcgtt ttgctgaaga tctgattttc tttaacaccg
    29341 gcgaagcggg gtttgtggag ctttctgacc gcgtgacttc cggttcatca ttaatgccgc
    29401 agaagaaaaa cccggatgcg ctggagctga ttcgcggtaa atgcggtcgg gtgcaggggg
    29461 cgttaaccgg catgatgatg acgctgaaag gtttgccgct ggcttacaac aaagatatgc
    29521 aggaagacaa agaaggtctg ttcgacgcgc tcgatacctg gctggactgc ctgcatatgg
    29581 cggcgctggt gctggacggc attcaggtga aacgtccacg ttgccaggaa gcggctcagc
    29641 agggttacgc caacgccacc gaactggcgg attatctggt ggcgaaaggc gtaccgttcc
    29701 gcgaggcgca ccatattgtt ggtgaagcgg tggtggaagc cattcgtcag ggcaaaccgc
    29761 tggaagatct gccgctcgac gagttgcaga aattcagccc ggtgattgac gaagatgtct
    29821 atccgattct gtcgctgcaa tcgtgcctcg acaagcgtgc ggcaaaaggc ggcgtctcac
    29881 cgcagcaggt ggcgcaggca attgcttttg cgcaggcgcg gttagagtaa tttgtttata
    29941 ttgctcccct gaaaatgggg agcttaccac gcctacaaag tgcgacactt cgtaggcgga
    30001 taaggcgttt acgccacatc aggcagaaat ttgtcagcat tctggtgagc ttaaatttaa
    30061 cgcccaacca gaaccattat ttgtcacatt cctttttatc attgtgtctt ctttaccttc
    30121 acactcccca taaaaatcat at
//
LOCUS       DAPHDW010000046        29721 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.046, whole genome shotgun sequence.
ACCESSION   DAPHDW010000046 DAPHDW010000000
VERSION     DAPHDW010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.046"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(212..2785)
                     /gene="clpB"
                     /locus_tag="RH057_002028"
     CDS             complement(212..2785)
                     /gene="clpB"
                     /locus_tag="RH057_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HDV3128397.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKAAGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
     gene            complement(2915..3646)
                     /gene="pgeF"
                     /locus_tag="RH057_002029"
     CDS             complement(2915..3646)
                     /gene="pgeF"
                     /locus_tag="RH057_002029"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HDV3128398.1"
                     /translation="MSKLIVPQWPLPKGVAACSSSRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAAHAGWRGLCAGVLEETVSCFADNPENILAWLGPAIGPR
                     AFEVGVEVREVFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="RH057_002030"
     CDS             complement(3643..4623)
                     /gene="rluD"
                     /locus_tag="RH057_002030"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HDV3128399.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISMLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            4758..5495
                     /gene="bamD"
                     /locus_tag="RH057_002031"
     CDS             4758..5495
                     /gene="bamD"
                     /locus_tag="RH057_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HDV3128400.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRVEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            5766..6107
                     /gene="raiA"
                     /locus_tag="RH057_002032"
     CDS             5766..6107
                     /gene="raiA"
                     /locus_tag="RH057_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HDV3128401.1"
                     /translation="MTMNITSKQMEITPAIRQHVADRLAKLEKWQTHLINPHIILSKE
                     PQGFVADATINTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVK
                     DANFVEEVEEE"
     misc_feature    6210..6335
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            6211..6258
                     /gene="pheL"
                     /locus_tag="RH057_002033"
     CDS             6211..6258
                     /gene="pheL"
                     /locus_tag="RH057_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheA operon leader peptide PheL"
                     /protein_id="HDV3128402.1"
                     /translation="MKHTPFFFAFFFTFP"
     gene            6357..7517
                     /gene="pheA"
                     /locus_tag="RH057_002034"
     CDS             6357..7517
                     /gene="pheA"
                     /locus_tag="RH057_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HDV3128403.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQQQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="RH057_002035"
     CDS             complement(7560..8681)
                     /gene="tyrA"
                     /locus_tag="RH057_002035"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HDV3128404.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGEVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="RH057_002036"
     CDS             complement(8692..9762)
                     /gene="aroF"
                     /locus_tag="RH057_002036"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HDV3128405.1"
                     /translation="MQKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHRFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGLLTARVA"
     gene            9972..10337
                     /locus_tag="RH057_002037"
     CDS             9972..10337
                     /locus_tag="RH057_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HDV3128406.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKGADENASTIRLN"
     gene            10487..11005
                     /locus_tag="RH057_002038"
     CDS             10487..11005
                     /locus_tag="RH057_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HDV3128407.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNGFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDALSRSGVKVNP
                     DVLMLARKKNDG"
     gene            10995..12221
                     /gene="dgcN"
                     /locus_tag="RH057_002039"
     CDS             10995..12221
                     /gene="dgcN"
                     /locus_tag="RH057_002039"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
                     /protein_id="HDV3128408.1"
                     /translation="MMDNDNSLNKRPTFKRALRNISMTSIFITMMLIWLLLSVTSVLT
                     LKQYAQKNLALTAATMTYSLEAAVVFADGPAATETLAALGQQGQFSTAEVRDKQQNIL
                     ASWHYTRKDPGDTFSNFISHWLFPAPIIQPIRHNGETIGEVRLTARDSSISHFIWFSL
                     AVLTGCILLASGIAITLTRHLHNGLVEALKNITDVVHDVRSNRNFSRRVSEERIAEFH
                     RFALDFNSLLDEMEEWQLRLQAKNAQLLRTALHDPLTGLANRAAFRSGINTLMNNSDA
                     RKTSALLFLDGDNFKYINDTWGHATGDRVLIEIAKRLAEFGGLRHKAYRLGGDEFAMV
                     LYDVQSESEVQQICSALTQIFNLPFDLHNGHQTTMTLSIGYAMTIEHASAEKLQEIAD
                     HNMYQAKHQRAEKLVR"
     gene            12237..12719
                     /locus_tag="RH057_002040"
     CDS             12237..12719
                     /locus_tag="RH057_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV3128409.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAMGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVITTP
                     "
     gene            complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="RH057_002041"
     CDS             complement(12796..13143)
                     /gene="rplS"
                     /locus_tag="RH057_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HDV3128410.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="RH057_002042"
     CDS             complement(13185..13952)
                     /gene="trmD"
                     /locus_tag="RH057_002042"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HDV3128411.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="RH057_002043"
     CDS             complement(13983..14531)
                     /gene="rimM"
                     /locus_tag="RH057_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HDV3128412.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="RH057_002044"
     CDS             complement(14550..14798)
                     /gene="rpsP"
                     /locus_tag="RH057_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HDV3128413.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="RH057_002045"
     CDS             complement(15047..16408)
                     /gene="ffh"
                     /locus_tag="RH057_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HDV3128414.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGKFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            16575..17366
                     /locus_tag="RH057_002046"
     CDS             16575..17366
                     /locus_tag="RH057_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HDV3128415.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLMICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLVHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            17387..18673
                     /locus_tag="RH057_002047"
     CDS             17387..18673
                     /locus_tag="RH057_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HDV3128416.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVHIGEYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="RH057_002048"
     CDS             complement(18728..19321)
                     /gene="grpE"
                     /locus_tag="RH057_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HDV3128417.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            19444..20322
                     /gene="nadK"
                     /locus_tag="RH057_002049"
     CDS             19444..20322
                     /gene="nadK"
                     /locus_tag="RH057_002049"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HDV3128418.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            20408..22069
                     /gene="recN"
                     /locus_tag="RH057_002050"
     CDS             20408..22069
                     /gene="recN"
                     /locus_tag="RH057_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HDV3128419.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALEIARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKFDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLNKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            22218..22559
                     /gene="bamE"
                     /locus_tag="RH057_002051"
     CDS             22218..22559
                     /gene="bamE"
                     /locus_tag="RH057_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HDV3128420.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(22621..22911)
                     /locus_tag="RH057_002052"
     CDS             complement(22621..22911)
                     /locus_tag="RH057_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417108.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HDV3128421.1"
                     /translation="MPGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="RH057_002053"
     CDS             complement(22901..23338)
                     /gene="ratA"
                     /locus_tag="RH057_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HDV3128422.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELASNMVQAFTVRAKEVYSAR"
     gene            23509..23991
                     /gene="smpB"
                     /locus_tag="RH057_002054"
     CDS             23509..23991
                     /gene="smpB"
                     /locus_tag="RH057_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HDV3128423.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            24206..24568
                     /gene="ssrA"
                     /locus_tag="RH057_002055"
     tmRNA           24206..24568
                     /gene="ssrA"
                     /locus_tag="RH057_002055"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(24729..27989)
                     /locus_tag="RH057_002056"
                     /pseudo
     CDS             complement(24729..27989)
                     /locus_tag="RH057_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571801.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
     gene            complement(28054..28173)
                     /locus_tag="RH057_002057"
     CDS             complement(28054..28173)
                     /locus_tag="RH057_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV3128424.1"
                     /translation="MLLSYVEYHHFIMMSLMSGEHNTLALSLQDESPGLVPVG"
     gene            complement(28259..28516)
                     /locus_tag="RH057_002058"
     CDS             complement(28259..28516)
                     /locus_tag="RH057_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HDV3128425.1"
                     /translation="MDARVVCKPNRLGRSMWHLVVLLEELCKRGINFRALAQSIFAQQ
                     WGDECCKSKRICDLKVIVLFLCEQKIFIRNDYVNHFILPTA"
     gene            complement(28902..29721)
                     /locus_tag="RH057_002059"
                     /pseudo
     CDS             complement(28902..29721)
                     /locus_tag="RH057_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961353.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         7547 a         7807 c         6808 g         7559 t
ORIGIN      
        1 cggaattctc aggagaaccc cgctgacccg gcggcgtgtt tgccgttgtt ccgtgtcagt
       61 ggtggcgcat tatagggagt tattccggcc tgacaagcga aaaatataaa aactttatcg
      121 ttcgctcatt tttcaggcaa aacatcttaa atatagtctt ttccgtctaa cttatagaca
      181 aaaacgagcc ccgaagggct cgttttatca tttactggac ggcgacaatc cggtcttcat
      241 taacttccag gcgaatcact ttacccggaa ccaattcacc agacagtatt tgctgtgcca
      301 gcgggttttc gatctgctgc tggatagcac gtttcaaagg acgtgcacca tagactggat
      361 cgtaaccgtt ctcgctcagc agtttcagcg cctcgtcaga aatgtggatt tcataaccac
      421 gttcttccag acgtttgtac agacgtttca actgaatctg cgcaatcgag gcaatgtgct
      481 gttcacccag cggatggaag accaccactt catcaatacg gttaatgaat tccggacgga
      541 agttatggct taccacaccg agcaccagct ctttcatgtg cgcataatcc agttcaccga
      601 agcgttcctg aatcaaatcg gaaccgaggt tagaggtcat aatgacaacc gtattacgga
      661 agtcgaccgt tctcccctgc ccgtcagtca gacgaccatc atccagtacc tgcaacagaa
      721 tgttgaagac atccggatgc gctttttcca cttcatccag caggatgacg gaatacggac
      781 gacgacgcac agcttcggtc aggtagccac cttcttcata accgacatat cccggaggcg
      841 caccaaccag acgagacacc gagtgtttct ccataaactc ggacatatcg atacggacca
      901 tcgcctcgtc gctatcaaac ataaagttcg ccagcgcctt acaaagctca gttttcccca
      961 cacccgttgg gccgaggaac aggaatgaac caatcgggcg atttggatcc gccaaccccg
     1021 cacggctacg gcgaatagcg ttagataccg catcaaccgc ttcgttctga ccaatgacgc
     1081 gatggtgcag ttcttgctcc atacgcagca gtttttcgcg ctcgctttcc atcatgcgag
     1141 aaaccggaat ccccgtccaa cgcgccagca cttcagcaat ttcggcgtcg gtcactttat
     1201 tacgcaacag acgcatagtt ttgccttcga gctgcgttgc ggcttccagt tgcttttcca
     1261 gttccgggat tttgccgtat tgcagttcag acatccgcgc caggtccccc acacggcgag
     1321 cctgttcaat agcgattttc gcctgttcca gttccgcttt aatggtctgc gtaccagaaa
     1381 gcgatgcctt ctctgctttc cactcttctt ctaactcgga gtactgacgt tctttgtcgc
     1441 tcagttcttc gttgagcata tccaggcgtt ttttactggc ttcatcagac tctttcatta
     1501 acgcctgttg ttccagtttg agctggatga tacgacgatc gagtcggtcg agttcttctg
     1561 gttttgagtc aatctgcata cgaatgctgg atgctgcttc atcgatcagg tcgatggctt
     1621 tatccggcag ctgacggtca gcaatgtagc gatgagacaa cgtcgccgct gcaacaattg
     1681 ccgggtcagt aatttgcaca tggtggtgca attcgtaacg ttctttcagg ccacgcagaa
     1741 tcgcaatggt atcttcaaca gaaggctcgg caacaaacac tttctggaaa cgacgttcca
     1801 gcgcagcatc tttttcgatg tactggcgat attcgtcaag cgtcgtggca cctacgcagt
     1861 gcaattcacc acgcgccagc gccggtttca gcatgtttcc ggcgtccatt gcgccatcgg
     1921 ctttacccgc gccgaccatg gtatgtaatt cgtcgataaa caggatgacg ttgccttcct
     1981 gtttggcaag atcgttaagc acgcctttta aacgttcttc aaactcaccg cgatatttcg
     2041 ccccagccac cagcgcgccc atatccagcg ccagtacgcg gcggcctttc aatccttccg
     2101 gcacttcgcc gttgataata cgctgcgcca gaccttcaac gatggcagtt ttaccgacgc
     2161 cgggttcacc aatcagtacc gggttatttt tagtacgacg ttgcagcacc tgaatggtac
     2221 ggcgaatttc ttcatcacga ccaatcaccg gatcgagttt gccctgttcg gctcgttcgg
     2281 taaggtcgat ggtatatttt ttcaaagcct gacgttggtc ttcagcacct tgatcgttca
     2341 cgctttcacc tccacgcatt tgttcaatcg cttgagtaat gttggcggtg gtcgcccctg
     2401 ctgctttcag gatgtcggcc agcgtgccgc gagactcaag tgccgccaga acgaacagtt
     2461 ctgacgagat aaagttatca ccacgttttt gcgccagctt gtcgcaaaga ttaagaacgc
     2521 gcaccagatc ctgtgatggc tggacatcac caccagtacc ttcaacctgc ggtaaacgat
     2581 ttaatgcctg attgatatct gtgcgcaact ggccagcatt tatgccagcg gatgttaata
     2641 aaggactaac cgaaccccct tcctgattca gcagggcgct cattaaatga agtggttcga
     2701 taaattggtt gtcgtgcccg agtgcaagtg attgggcatc ggcaagagca agctggaatt
     2761 tattagtaag acgatccaga cgcataactc ctcccataac ggatcaaagt tgctactgga
     2821 gattaaatga ggtcatccct caattattca aggttattga ccagattaat gtgaaaagaa
     2881 aatcacgcgt accggatcgt cttgattctt taggttatat cagccaaatg aaacttgcca
     2941 tacgaccggt ggtcttgtcg cgacgataag agaagaaagt ctcattttcc gtatatgtac
     3001 aacggtcgcc gccgaaaatt tgctcaacac ccacgttcgc cagacgctgc cgggcaagct
     3061 gataaatatc cgccagatac ttatcaccat gctgaatgaa agctgcactt gctttagcgt
     3121 ctactgccat aaacacctcg cgaacctcca cccccacttc gaacgcgcgt ggaccaattg
     3181 ccggccctaa ccaggcgaga atattttccg gattatcagc aaaacaggaa accgtctctt
     3241 ccagcacgcc tgcgcacagt ccacgccagc cagcatgagc ggcggcgact tccgttcccg
     3301 ctcgattgca aaacagcaca gggaggcagt cggcagtcat cactgcgcaa accgtgccgg
     3361 gcgtattgct ataagaggca tctgctcgtt ttgaggcata aggttcgcca gtaagcttaa
     3421 gcacatcttt gccgtgtacc tgctcaagcc agaccggttt agaaggcaaa ttgcccgcag
     3481 caaaaagtcg cttgcgattc tcctcaacgt gatccgggtt atcgccacaa tgggcaccga
     3541 ggttgagcga gtcatacggg ggcaagctca cgccgccgat acgagaagag ctacaggccg
     3601 caacaccttt tggtagcggc cactgcggga caatcagctt actcataacc agtccacttc
     3661 atccttatgc tcttcgaaat cggcgcgcat cacctcaatc agctccacca tatcttgtgg
     3721 aataggcgca tgccattcca tttcgatgcc ggagatcggg tgataaagac gcagcatggt
     3781 tgcatgaagc gcctggcggt caaacttacg cagcatggag ataaatgctt ccgaagcacc
     3841 ttttggcgga cgcggacggc caccataaac cggatcgccc accagcggat gagtgatatg
     3901 ggccatatgc acgcggatct ggtgcgtacg tccagtttcc agacgcaacc gcagacgcgt
     3961 gtgcacacgg aagtgttcca tgatgcgata gtgagtcacc gccggtttgc ccatcggatg
     4021 caccgccatg tgagtacgtt tggtcgggtg gcggctgatg ggttcatcta ctgtaccgcc
     4081 agcggtcata tgaccaatcg ccaccgcttc atactcacga gtaatttcac gccgttgcaa
     4141 agattcgact aaacgcgtct gagccggaac ggtttttgcc acaaccatca ggccagtggt
     4201 gtctttatcc agacgatgga cgatgcccgc acgtggtaca tcggcaatgg gcggatagta
     4261 atgaagcaac gcattcagta ccgtgccatc cgggttaccc gcgccaggat gtaccaccag
     4321 gtcgcgcggt ttattaatga cgatgatatc ttcatcttca tagacgatat ccagcgggat
     4381 atcctgcggt tcaaaacgcg cttcttcttc aatctcagcg ttgatggcaa cctgctcgcc
     4441 acccaatact ttttctttcg gcttatcaca aactttgccg tttaccagca cgcgctggtc
     4501 gaggatccat tcttttattc gcgaacgtga ataatccggg aacatttcgg ccaaagcctg
     4561 atctaagcgt tgaccgagtt ggttttcgga caccgttgca gtgagctgta ctcgttgtgc
     4621 catatatact gcttcttcgt ttatcgttgg ggttttacgg ctttgccgtt taatatagtg
     4681 tgctattgta gctggtctta accgggagca ggaacagaga atctcccgta ttacattttg
     4741 aggaaagtca aaacgtcatg acgcgcatga aatatctggt ggcagccgcc acactaagcc
     4801 tgtttttggc gggttgctcg gggtcaaagg aagaagtacc tgataatccg ccaaatgaaa
     4861 tttacgcgac tgcacaacaa aagctgcagg acggtaactg gagacaggca ataacgcaac
     4921 tggaagcgtt agataatcgc tatccgtttg gtccgtattc gcagcaggtg cagctggatc
     4981 tcatctacgc ctactataaa aacgccgatt tgccgttagc gcaggctgcc atcgatcgtt
     5041 ttattcgcct taacccgacc catccgaata tcgattatgt catgtacatg cgtggcctga
     5101 ccaatatggc gctggatgac agtgcgctgc aagggttctt tggcgttgac cgtagcgatc
     5161 gcgatcctca acatgcacga gctgcgttta gtgacttttc caaactggtg cgcggctatc
     5221 caaacagtca gtacaccacc gatgccacca aacgtctggt attcctgaaa gatcgtctgg
     5281 cgaaatatga atactccgtg gccgagtact atacagaacg tggcgcatgg gttgccgtcg
     5341 ttaaccgcgt agaaggaatg ttgcgcgact acccggatac ccaggctacg cgtgatgcgc
     5401 tgccgctgat ggaaaatgca taccgtcaga tgcagatgaa tgcgcaagct gaaaaagtag
     5461 cgaaaatcat cgccgcaaac agcagcaata cataacagaa acctgaaata caaaacggca
     5521 gcccttgagc tgccgttttt ttattctgtt agttgtgaaa ctgaagcgat ttagtcacta
     5581 tcgatctcat caaatatggc tcgctttgag atattcctca agtaaaaaaa cacctcttcc
     5641 tgcgatttct cacaaaaaag attcgttgac aaaaagtgac aaaattatga gattcccatc
     5701 acacattttg acatcaggag cggtatgctg aattcaccaa gacgggaaga caagaggtaa
     5761 aatttatgac aatgaacatt accagcaaac aaatggaaat tactccggcc atccgccaac
     5821 atgtcgcaga ccgtctcgcc aaactggaaa aatggcaaac acatctgatt aatccacata
     5881 tcattctgtc caaagagcca caagggtttg ttgctgacgc cacaatcaat acacctaacg
     5941 gcgttctggt tgccagtggt aaacatgaag atatgtacac tgcaattaac gaattgatca
     6001 acaagctgga acggcagctc aataaactgc agcacaaagg cgaagcacgt cgtgccgcaa
     6061 catcggtgaa agacgccaac ttcgtcgaag aagttgaaga agagtagtcc tttatattga
     6121 gtgtatcgcc aacgcgcctt cgggcgcgtt ttttgttgac agcgtgaaaa cagtacgggt
     6181 actgtactaa agtcacttaa ggaaacaaac atgaaacaca caccgttttt cttcgcattc
     6241 ttttttacct tcccctgaat gggaggcgtt tcgtcgtgtg aaacagaatg cgaagacgaa
     6301 caataaggcc tcccaaatcg gggggccttt tttattgata acaaaaaggc aacactatga
     6361 catcggaaaa cccgttactg gcgctgcgag agaaaatcag cgcgctggat gaaaaattat
     6421 tagcattact ggcagagcgg cgcgaattgg ccgtcgaggt aggaaaagcc aaactgctct
     6481 cgcatcgccc ggtacgagat attgatcgtg aacgcgattt actggaaaga ttaattacgc
     6541 tcggtaaagc gcaccatctg gacgcccatt acattactcg cctgttccag ctcatcattg
     6601 aagattccgt attaactcag caggctttgc tccagcaaca tctcaataaa attaatccgc
     6661 actcagcacg catcgctttt ctcggcccca aaggttctta ttcccatctt gcggcgcgcc
     6721 agtatgctgc ccgtcacttt gagcaattca ttgaaagtgg ctgcgccaaa tttgccgata
     6781 tttttaatca ggtggaaacc ggccaggccg actatgccgt cgtaccgatt gaaaatacca
     6841 gctccggtgc cataaacgac gtttacgatc tgctgcaaca taccagcttg tcgattgttg
     6901 gcgagatgac gttaactatc gaccattgtt tgttggtctc cggcacgact gatttatcca
     6961 ccattaatac ggtctacagc catccgcagc cattccagca atgcagcaaa ttccttaatc
     7021 gttatcctca ctggaagatt gaatataccg aaagtacgtc tgcggcaatg gaaaaggttg
     7081 cacaggcaaa atcaccgcat gttgctgcat tgggaagcga agctggcggc actttgtacg
     7141 gtttgcaggt actggagcgt attgaagcga atcagcaaca aaacttcacc cgatttgtgg
     7201 tgttggcacg taaagccatt aacgtgtctg accaggttcc ggcgaaaacg acgttgttaa
     7261 tggcgaccgg gcaacaagcc ggtgcgctgg ttgaagcgtt gctggtgctg cgcaaccaca
     7321 atctgattat gacccgtctg gaatcacgcc cgattcacgg taatccatgg gaagagatgt
     7381 tttatctgga tattcaggcc aatcttgaat cagcggaaat gcaaaaagca ttgaaagagt
     7441 taggggaaat tacccgttca atgaaggtat tgggctgtta cccaagtgag aacgtagtgc
     7501 ctgttgatcc aacctgatga aaaggtgccg gatgatgtga atcatccggc actggattat
     7561 tactggcggt tgtcattcgc ctgacgcaat aacacgcggc tttcactctg aaaacgctgt
     7621 gcgtaatcgc cgaaccagtg ctccaccttg cggaaactgt caataaacgc ctgcttatcg
     7681 ccctgctcca gcaactcaat cgcctcgccg aaacgcttat agtaacgttt gattaacgcc
     7741 agattacgct ctgacgacat aataatgtcg gcataaagct gcggatcctg agcaaacagt
     7801 cgcccgacca tcgccagctc aaggcggtaa atcggcgaag agagcgccag aagttgctca
     7861 agctgaacat tttcttctgc cagatgcagc ccataagcaa aagtagcaaa gtggcgcaga
     7921 gcctgaataa acgccatatt ctgatcgtgc tcgacagcgc taatacgatg cagccgagcg
     7981 ccccagacct gaatttgctc cagaaaccat tggtatgctt ccggcttacg tccatcacac
     8041 cagaccacaa cttgctttgc caggctaccg ctgtccgggc cgaacatcgg gtgtaacccc
     8101 agtaccgggc catcgtgcgc cgccagcatg gcctgtaatg gtccattttt cactgatgcc
     8161 agatcaacca gaatacaatc tttcggtaaa ggcggtaatt tgccgataac ttgctcagta
     8221 acgtggattg gcacactaac aatcaccatt ccggcatcgg caacaatatc agccgctcga
     8281 tcccagtcat gttgctccag aatccgcacc tgataacccg agagggtcag catcttctcg
     8341 aacaggcgtc ccatctgacc gccaccgccg acgataacca ccggacgcag tgacggacaa
     8401 agtgttttaa atcctttgtc gttttcactg gagtaagatt cacgcatcac ccgacgcaaa
     8461 acatcctcaa tcagatctgg cggtacaccc agagcttccg cctctgcacg acgcgaggcc
     8521 aacatagatg cctcgcgctc cggaacataa ataggcagtc caaagcggct tttcacctcg
     8581 cccacttcag caaccagttc cagacgctta gctaataaat tcagcagcgc tttatcgact
     8641 tcatcaattt gatcgcgtaa tgcggtcaat tcagcaacca taataaacct cttaagccac
     8701 gcgagccgtc agcagcccgt tcagatcctg atgaatttca cgcagcaagg catcggtcat
     8761 ttcccagcta atgcaggcat cggttacgga tacaccgtat ttcatttcac tgcgcggttg
     8821 ctcggaagac tgattgccct cgtggatatt actttcgatc atcagaccaa taattgagcg
     8881 attgccatct ttgatttgag caaccacgga ttctgccacc gcaggctgac ggcgataatc
     8941 tttattggaa ttaccgtggc tgcaatctac catcagagac gggcgcagtc ccgcctgttc
     9001 catctctttt tcacattgcg caacatccgc agggctatag ttcggcgctt taccaccgcg
     9061 caggatgaca tgcccgtccg gattcccctg agtttgtagc aacgcaacct gccctgcctg
     9121 gttaatgcca acaaaacggt gcggctgggc ggcggcgcgc atagcgttaa ttgctgttgc
     9181 cagactgccg tcggtgccgt ttttaaaacc aaccggcatg gaaagcccgg aggccatttc
     9241 acggtgagtt tgcgattccg ttgtacgagc accaattgct gaccagctaa acagatcgcc
     9301 caggtattgc gggctattcg gatctaacgc ttccgtcgcc agtggcagtc ccatattcac
     9361 cagctcaagc agcaatttac gcgcgatctg cagcccggct tctacatcaa aagagccatc
     9421 catatgggga tcgttaatta accctttcca gccgacagtg gtacggggtt tttcaaaata
     9481 gacgcgcatt accagataga ggctatcgct gacctctgcg gcaagggctt taaatcgacg
     9541 agcatattcc agagcagttt ccggatcatg aatggaacaa ggaccacata ctaccagcag
     9601 acgaggatcg cgcccggcga taatatctga aatggtttta cgcgagtcag caatctgggc
     9661 ttcttgttgc aggctcaatg gaaaagcggc cttcagttgt tccggagtca ttaaaacctg
     9721 ttcgtcggta atatgtacgt tattcagcgc gtctttttgc atgatggcga tcctgtttat
     9781 gctcgtttgc gatagttgat cctcagcgag gatgacgtaa cgataacaca taaagtaaag
     9841 ttttcaatcc atatttcgta catttttatt tacacaggca atttagtcgc gatttcaacc
     9901 cttacttctg tatagataaa tttacacttc ctttgaaaac aatccgctat gctttgaaaa
     9961 aggagaaaga aatgatgaaa aagtttatcg cccccttgtt ggctttactg gttagcggat
    10021 gtcagattga tccttatact cacgcgccaa ccttgaccag caccgactgg tatgatgttg
    10081 gtatggaaga tgcgatatcg ggcagcgcca taaaagatga cgatgcattt agcgattcac
    10141 aggcggatcg cggtctatac cttaaaggat atgccgaagg acaaaagaaa acttgccaga
    10201 ccgattttac ttatgcccgg ggactttccg gtaaaagctt tcctgccagc tgcaacaatg
    10261 ttgaaaatgc cagccaactg catgaagtat ggcaaaaagg ggctgatgaa aatgccagca
    10321 caatacgatt aaattaatta aaaccccaca aataaattaa acataagatt ttactcattg
    10381 tgttttattt ctcacattga tgacgatcgc atgaaaaaat gatattttaa tgagcattct
    10441 gaaaaaatat cgatgcattt cgagcgaaga tggtggggat ccctgaatgc gtttttctca
    10501 ccgactgttc ttacttctga ttctcctgct gacgggagca ccaatactcg ctcaggagcc
    10561 ttctgacgtt gcgaaaaacg tgcgtatgat ggtttccggt attgtcagtt acacccgctg
    10621 gcctgcgcta tcagggccac caaagttatg tatcttttca tcttcacgtt ttagtaccgc
    10681 cctacaagag aatgccgcga catctttgcc ttatctgcct gtcatcattc acactcaaca
    10741 agaggcgatg atttcaggct gcaatggttt ttattttggc aacgagtcac cgacattcca
    10801 gatggaatta accgaacaat atccgtcaaa ggcgttatta ttaattgccg aacaaaatac
    10861 cgagtgcatt attggtagcg ccttttgtct gatcatccac aataatgacg tcagatttgc
    10921 cgtaaacctg gatgccttat cgcgtagcgg tgtaaaggtc aacccggatg tcttaatgct
    10981 cgcacggaag aaaaatgatg gataacgata attctcttaa taagcgcccc acgtttaaaa
    11041 gagcattacg caacatcagt atgaccagca tatttatcac tatgatgctg atctggttgc
    11101 tgctttccgt gacctcggtg ctgaccctga aacagtacgc gcaaaaaaac ctggcactga
    11161 cagcagcaac aatgacttac agtctggaag cagctgtcgt ttttgccgat ggccctgcag
    11221 caactgaaac actggcagcg ctgggccagc aagggcaatt ttcaactgca gaagtacgtg
    11281 ataagcagca aaatattctg gcgtcctggc attacacccg taaggatcca ggcgatactt
    11341 tcagcaattt cataagccac tggctcttcc ccgcccccat cattcagccg attcgtcaca
    11401 atggtgaaac cattggcgaa gtacgcttaa ccgctcgcga cagttcaatc agccatttta
    11461 tctggttttc gctcgccgta ctgaccggtt gtattctgct ggcatcaggc atcgcaatta
    11521 ccctcacccg ccatttgcac aatggcctgg tagaagcgct gaaaaatatc accgatgtcg
    11581 tacatgatgt gcgttccaac cgcaattttt cccgacgagt ttcggaagaa cgtatcgctg
    11641 agtttcaccg cttcgctctc gacttcaaca gtctgctgga tgaaatggaa gagtggcagc
    11701 ttcgtttaca ggccaaaaat gcgcagcttc tgcgtaccgc gctacatgac ccattaaccg
    11761 ggctggctaa ccgcgcagcg tttcgtagcg gcatcaatac gttgatgaac aattccgatg
    11821 cccgaaaaac gtcggcgtta ctatttcttg atggcgataa tttcaaatat atcaatgata
    11881 cctggggtca tgcgacgggc gatagagtct tgattgaaat cgcaaaacgg ttagctgaat
    11941 ttggcgggct gcgacataaa gcataccgcc tgggcggcga tgaattcgct atggtgctct
    12001 atgatgtaca gtcggaatct gaagtgcagc agatatgctc agcactgaca caaatcttta
    12061 atctcccgtt tgatcttcat aatggccatc agaccaccat gacattaagc attggttacg
    12121 cgatgaccat tgagcacgcc tctgcggaaa aattacaaga gattgccgat cacaatatgt
    12181 atcaggccaa acaccagcgt gccgaaaagc tggtgagata acaaggatat atcgatatga
    12241 taaagcacct ggtagcaccc ctgattttca cctcactaat actgactggc tgccagtccc
    12301 ctcagggaaa gtttactcct gagcaagtcg ccgctatgca atcttatgga tttactgaat
    12361 ccgccggcga ctggtcgctg ggcttatcag atgccattct gttcgcaaaa aatgactaca
    12421 aattgctccc ggaaagccag caacagatcc aaaccatggc agccaaattg gcctcgacag
    12481 ggctaacaca tgcccgtatg gatggacaca ccgataacta tggtgaagac agttacaacg
    12541 aaggcttatc attgaaacgg gcgaatgtcg tggccgatgc atgggctatg ggtggacaaa
    12601 ttccacgcag caatctcacc acacagggtt taggaaaaaa atatcccata gccagtaaca
    12661 agaccgccca gggccgcgcc gagaaccgcc gcgtcgcagt ggtgattaca accccttaaa
    12721 acacgagcaa acagacaaaa aaagggccag ccaattggcc agcccttctt aacaggatgt
    12781 cgcttaagcg aaatcttagt taagacgctc tttgatacga gcagccttac cagtacgctc
    12841 acgcaggtag tacagtttag ctttacgaac agcaccacga cgtttgacag aaatgctgtc
    12901 aactaccgga gagtgagtct ggaagacacg ctcaacgcct tcgccgttgg aaattttacg
    12961 aacagtgaat gcagagtgca gaccgcggtt acgaatagcg ataaccacgc cctcgaatgc
    13021 ctgcagacgt tttttggaac cttcaacaac ccatactttc acttccacgg tatcacccgg
    13081 acggaaggaa ggtacgtcct gcttcatctg ctcttgttca agttgcttaa taatgttgct
    13141 cataatttaa tctcttatcc tgggtaaact gatatctcgg gggcttacgc catcccatca
    13201 tgtttatgtt gctgttgtgc gtgttccgtt ttgaactccg ccagcaacct tgcttgctct
    13261 tcagtcagag ccaggttttc cagaagttca ggtcttctaa gccaggtacg gcccagcgac
    13321 tgtttcaaac gccagcgacg tatctcggca tggttgcccg acagtaacac tggcggaact
    13381 tccatccctt ctaacacctc aggccgcgta tagtgcgggc aatccagcaa tccttcagca
    13441 aaggaatctt ccgttgccga ggcttcatgt cccagtactc ccggaataaa ccgggaaacg
    13501 gagtcaatca gcgtcattgc tggtaactca ccaccactga gaacgtaatc gccgattgac
    13561 cattcttcgt caatttcggt ttggatcacg cgctcatcta taccttcgta gcgaccgcac
    13621 accagaatca atttttgatt cgttgccagt tcgctgacgc ccgcttgatc aagcttgcgt
    13681 ccctgtggtg acagataaat cacctttgcg ccttcacccg ccgcggcttt tgctgcatga
    13741 atggcgtccc gcaagggttg caccatcatt aacatccccg gtccgccgcc gtaaggacga
    13801 tcgtccacgg tacggtgccg gtcatgcgtg aagtcgcgag gactccaact ctggatgctc
    13861 agcaggccat ttttaactgc ccggccagtt accccgtaat cggtaattgc gcggaacatt
    13921 tcaggaaaca ggctaattat gccaatccac atagcgccgt cttttaccgt ttatccggtg
    13981 gtttaaaaac caggatccca atctacttcg attgaacgag tagtgagatc gactttcttg
    14041 ataacctgcc catcgaggaa cggtacgaga cgttccttga taccaaacgc atctttcagg
    14101 tttgccttaa tgacgagaac gtcattagat ccggtttcca tcatatcgac gactttaccg
    14161 agatcgtagc cttcagtggt tactacctgg cagcccatca ggtctttcca gtagtagtca
    14221 ccctcttcaa gctgaggcag ctgcgatgaa tccacgacaa tttcacaatt cgtcagcagg
    14281 ttcgccgcat cacgatcgtc aacgcctttc agcttgatga tcatgtcctg attgtggtgc
    14341 ttccagcttt ccagctggac ttgctgccac tgacccgcct tctggataaa ccagggctga
    14401 tagtcaaaaa tgctttcggc gtcttcggtg gaagaaaaca ctctgagcca cccacgaata
    14461 ccgtaagacg aacccatttt tcccaaaacg atgggatcaa caggtgcttg cgcggtgagt
    14521 tgtttgctca tcatgaccac cgtgacagat taagctgctt tgtttacttc tttgatcagc
    14581 gcagcaacgc gatcagaaat agttgcgccc tggccaaccc agtgagcgat gcgatccaga
    14641 tccaggcgag tgccttcttc tttttcgcta gcgattgggt tgaagaaacc aacgcgctcg
    14701 atgaagcgac cgttgcgtgc attacggctg tcagcgacaa caacctggta gaacggacgc
    14761 tttttagcgc cgtgacgtgc taaacgaata gttaccataa catcctcttg tgtgaataaa
    14821 acaaccggac cccatcgagg aacggagtcc ggtgtcatat taaaagcccg aaaattttac
    14881 tcatttttgc gggaattgca atcaacagtt gctaactctg ctgtaaaagg ccgtcggcgg
    14941 tgcagccagt ttggtgccgg agtgcgcgca gtcaccggag cgtacacgca gtacgtgagg
    15001 atgacgagca catcccggtg ccaaaatggc aaacaagcca ggccgattag cgaccaggga
    15061 agcctggggg catcataccc ttcatgcttc tcatcatctt cgccattccg cccttcttca
    15121 ttttcttcat catgcgctgc atgtcgtcga actgtttcag aagacggtta acgtcctgca
    15181 cctgcatacc gcaaccggca gcaatacggc gtttacgcga acctttgatg atttctggct
    15241 tagcgcgctc tttcatcgtc atcgagttga tgatggcttc catacgcacc agcactttat
    15301 cgtccatctg cgacttaaca ttatccggga tctgccccat gcccggcagc ttgcccatca
    15361 ggctagccat gccgcccata tttttcatct ggcgcagttg ctcaagaaaa tcgttgagat
    15421 cgaagccgtc gccttttttc agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt
    15481 tgctttcgat atcttcgatc agcgacagta cgtcgcccat gccgagaata cgcgacgcga
    15541 tgcggtccgg atggaacggc tccagcgcct cagttttctc gccaacaccg agaaacttga
    15601 tcggtttgcc agtgatgtga cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca
    15661 ctttggtcaa cactacgccg gtaagcggta acgcttcatt gaatgctttt gccgtattgg
    15721 ccgcatcctg accggtcatg gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg
    15781 catggacttg tttgatctcg tccatcatcg cttcgtcaac gtgcagacga ccagcggtat
    15841 ccaccagcag cacgtcgtag aatttcagtt tggcttcttt cagcgccgcg ttaacgatat
    15901 ctaccggctt ctgaccaaca tcagaaggga agaaatcaac gcccacctgc tctgccagcg
    15961 tctcaagctg tttgattgcc gccgggcgat aaacgtcggc agaaaccacc agcactttct
    16021 tcttgtgctt ctcgcgcagg aacttaccga gtttaccaac gctggttgtt ttaccggcac
    16081 cttgcaggcc cgccatcagc acgaccgcag gcggttgcgc cgccaggttc agggtctggt
    16141 tctcttcgcc catcgccgca accagttcgt tacggacgat tttgacgaac tcctgccccg
    16201 gcgtcaggct cttattaact tcatgaccaa ccgctttctc ttttacgcga ttgataaact
    16261 cacgcactac cggcagagcg acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg
    16321 tatcttttac gttgtcttca gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg
    16381 acaaacgatc ggttaaatta tcaaacattg tctctcgcct ggggtggaaa cggttggccg
    16441 caatcgcgac acatcatcag tattttgccg cagtataaca tgaaggcgtc tttgttgtta
    16501 tgcaacggtt ggagcagcgt tcacctgacg ctatactgct tctctttctt attgctcaaa
    16561 ctgtcgacat cactatgccc gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc
    16621 ttgcgctgat tgttcccggt ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta
    16681 tttctgcggt cattgcgctg gtctgccacg caatcgctct ggaagcccgc atcctgcccg
    16741 acggtgatag cggacaaaac ctcagcctgc tgaacgttgg ttcattggtc agtttgatga
    16801 tctgtacggt aatgaccatt gtggcttctc gcaatcgtgg ctggttgctg ctacccattg
    16861 tctatgcctt tgcgcttatc aacctggcgc tggcgacctt catgcccaat gaatacatca
    16921 cccatctgga agcaacgcct gggatgctgg tgcacattgg cttatcgctc ttttcctatg
    16981 ccacgctaat tatcgccgcc ctgtacgcgc tgcaactggc gtggattgat taccaactga
    17041 agaacaagaa gctggcattt aaccaggaaa tgccgccatt gatgagtatc gagcgtaaaa
    17101 tgttccacat cacgcagatt ggcgtggtgc tgctaacgct cacgctttgc actggcctgt
    17161 tctacatgca caacctattt agcatggaaa atatcgacaa ggctgtgctc tctatcgtgg
    17221 cgtggtttgt ctatattgtg ctgctgtggg gacattacca tgaaggatgg cgtggacgcc
    17281 gcgtcgtctg gtttaacgtt gcgggcgcgg taattctgac actggcctac ttcggcagcc
    17341 gaattgtcca gcagttaatc agctaaaccc agaaaaggag tttcccctgg aacacatttc
    17401 tactactacg ttgatcatta ttctgatcat catggtggtc atttcagcct atttttccgg
    17461 gtccgaaacc ggaatgatga ccctcaaccg ctatcgtctg cgacatatgg cgaaacaggg
    17521 taatcgctcg gccaaacgcg tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct
    17581 ggtgttaatc ggcaataacc tggtcaatat tcttgcctcc gcgctcggca ctattgttgg
    17641 gatgcgtttg tacggcgatg cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt
    17701 actggtattt gctgaggtat tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc
    17761 ttatccgagt agttttctgc tggctccgct gcaaattttg atgatgccgc tggtctggtt
    17821 gctgaatgct atcacccgca tgttaatgcg catgatgggc atcaaaaccg atatcgtggt
    17881 cagcggctct ttgagcaaag aagagttgcg cactatcgtg catgaatcgc gctcacaaat
    17941 ttcccgtcgc aatcaggata tgctgctgtc ggtgctcgat ctggaaaaaa tgaccgttga
    18001 tgacatcatg gtgccgcgca gtgaaattat cggtattgat atcaacgatg actggaaatc
    18061 gattctgcgc caactctccc actcacctca cgggcgcatc gtgctctacc gtgattcgct
    18121 ggacgacgcc atcagtatgc tgcgtgtgcg tgaagcctgg cggttgatgt cggagaaaaa
    18181 agagttcacc aaagaaacca tgctacgcgc cgcggacgag atctattttg tcccggaagg
    18241 tacgccgctc agcacgcagt tggtgaaatt tcagcgcaac aaaaagaaag tcggcctggt
    18301 cgtcaacgag tatggagaca ttcaggggct ggtgacggtt gaagatattc tggaagagat
    18361 tgtcggcgat ttcactacgt cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa
    18421 cgacggttcg gtgattattg atggcactgc caacgtgcgg gaaatcaaca aagcctttaa
    18481 ctggcatcta cctgaagacg atgcccgcac ggttaatggc gtcattcttg aagctctgga
    18541 agagatccct gtcgctggca cccgcgtgca tattggcgag tacgatatcg atattctcga
    18601 cgttcaggac aatatgatta agcaggtaaa agtctttcct gtaaaaccgc tgcgcgagag
    18661 tgtggcggag taacgaaaac ggccctgcat tcgcatgcag ggccgtgaat tattacgaaa
    18721 acagaaatta cgcttttgct ttcgctacag ttaccatcgc cgcacgaatc gtacgaccat
    18781 tcagcgtata acccttctgc ataatgccca gtacgttacc tggcgcaacg tcatcagatt
    18841 ccaccattgc gatagcctga tgcacattcg ggtccagcgg cacgttagtt tcggcgatca
    18901 cttcaacacc aaacttacgc acaacatcca gcatcgactt cagcgtcagc tcaatgcctt
    18961 caaccatcgc agacatatcc gggttagctt tatcagccac ttccagcgca cgatccaggc
    19021 tatcaatcac cggcagcaat tcgttgatga atttctccag cgcgaatttg tgggcttttt
    19081 caatatccag ttcagtacgt cgacgcaggt tttccatttc ggcttttaca cgcaaaatgc
    19141 cgtcacgttc acgggtctgg gcttcagcca gctgagcttc gagattcgca attttttcat
    19201 cgcgcggatc cacctgctca gcagaagctt ctggctcaac tgcctcaatc tcttcgtgct
    19261 gatccatgat aatttcttcc ggggcttgcc cctcaggcgt tttctgttct ttactactca
    19321 tgaatttctc cgcgtttttt tcgcattcat ctcgctaact tcgcttatta tggggatcag
    19381 tttcagggtt tcaagggaag cactcacatt gtcatcaatc ttcgcaacaa ggacctcgga
    19441 aaaatgaata atcatttcaa gtgtattggc attgtgggac acccacggca ccctactgca
    19501 ctgacaacac atgaaatgct ctaccgctgg ctgtgcacaa aaggttacga ggtcatcgtt
    19561 gagcaacaaa tcgctcacga actgcaactg aagaatgtga aaactggcac gctcgcggag
    19621 attgggcaac tagctgatct cgcggtagtc gttggtggcg acggtaatat gctgggcgcg
    19681 gcgcgcacac tcgcccgtta cgatattaaa gttattggaa tcaaccgtgg caacctgggt
    19741 ttcttgactg accttgaccc cgataacgcc cagcaacagt tagccgatgt gctggaaggc
    19801 cactacatca gcgagaaacg ttttttgctg gaagcgcaag tctgtcagca agattgccag
    19861 aaacgcatca gcaccgcgat aaatgaagtg gtgcttcatc caggcaaagt ggcgcatatg
    19921 attgagttcg aagtgtatat cgacgagatc tttgcgtttt ctcagcgatc tgatggacta
    19981 attatttcga cgccaacagg ctccaccgcc tattccctct ctgcaggcgg tcctattctg
    20041 accccctctc tggatgcgat taccctggtg cccatgttcc cgcatacgtt gtcagcacga
    20101 ccactggtca taaacagcag cagcacgatc cgtctgcgtt tttcgcatcg ccgtaacgac
    20161 ctggaaatca gttgcgacag ccagatagca ctgccgattc aggaaggtga agatgtcctg
    20221 attcgtcgct gtgattacca tctgaatctg attcatccga aagattacag ttatttcaac
    20281 acattaagca ccaagctcgg ctggtcaaaa aaattattct aattttacgc cagcctcttt
    20341 actgtatata aaaccagttt atactgtaca caataacagt aatggttttt catacaggaa
    20401 aacgactatg ttggcacaac tgaccatcag caactttgct atcgttcgtg agcttgagat
    20461 tgattttcat agcggcatga ccgtaataac tggcgagacc ggcgcgggta aatctattgc
    20521 aatagatgcc ctcggtcttt gtctcggtgg tcgcgctgaa gccgacatgg tgcgtaccgg
    20581 cgctgctcgc gctgacctgt gcgcccgttt ttctctgaaa gatacgccag cggctctgcg
    20641 ctggctggaa gaaaaccagc ttgaagacgg gcatgaatgt ttgcttcgtc gcgtgatcag
    20701 cagcgatggt cgctcccgtg gtttcatcaa cggtacagct gttcctctgt cacaactgcg
    20761 cgaactgggt cagttgctga ttcagatcca tggtcagcac gctcatcagt tactcaccaa
    20821 acccgagcac caaaagttcc tgcttgatgg ctatgccaat gaaacctctc tactccagga
    20881 aatgacagca cgttatcagt tgtggcatca aagctgccgt gacctcgcgc atcatcaaca
    20941 gttaagtcag gaacgcgctg cccgtgcgga actgctgcaa taccaattaa aagaacttaa
    21001 cgaatttaat ccgcagcccg gagagtttga acaaatcgac gaagagtaca aacgtctggc
    21061 gaacagcggt caattgctga ccaccagcca gaatgcattg gcattaatgg ccgacggtga
    21121 agacgcaaac ctgcaaagtc agctttacac ggctaaacaa ctggtgagcg aattgattgg
    21181 catggacagc aaactgtccg gcgtacttga tatgctggaa gaagctacca tccagattgc
    21241 tgaagccagc gatgaactgc gccactactg cgatcgtctg gatctcgatc ccaaccgact
    21301 atttgaactt gaacagcgca tctcaaaaca gatttcgctg gcacgtaaac atcacgtcag
    21361 ccctgaggca ttgccacagt attaccagtc gctactggaa gaacagcagc aactggacga
    21421 tcaggccgac tcacaagaaa cgcttgcgct ggcggtaacg aaacatcatc agcaggcact
    21481 ggaaatcgcg cgcgcattac accaacaacg ccagcaatat gcagaagaac ttgcacagct
    21541 gatcaccgac agtatgcatg cgctctcaat gccgcatggg cagtttacga tcgatgttaa
    21601 atttgacgag catcacctgg gcgctgacgg tgccgatcgt attgagtttc gggtaaccac
    21661 caacccaggt cagccaatgc agcctattgc caaagtcgca tccggtggtg aattgtcccg
    21721 catcgcactg gcaatccagg tcatcacggc gcgtaaaatg gaaaccccgg cactgatttt
    21781 tgatgaagtg gatgtaggta ttagcggtcc aacagcggca gttgtcggca aactgctgcg
    21841 tcaactcggc gaatcaactc aggtgatgtg tgttacccac ctgccacaag tcgcgggatg
    21901 tggtcatcaa cactattttg tcagcaaaga aaccgatggt gcgatgacag aaacgcatat
    21961 gcaatccctg aataaaaaag cgcggttaca agagctggcg cgcctgcttg gtggcagtga
    22021 agtcacacgt aatacactgg cgaatgcgaa agaactgctt gcagcgtaaa cttttttcct
    22081 gcttcacggt cagagtaaac agcaaaacgc cgtaagaccg gaaagcaaaa ggttttaaag
    22141 tgatgaaagg tctattatca tcggcatatt acagatgagc cacgtactgc tcgggcccga
    22201 aaaggaatca aatcactatg cgctgtaaaa cgctgactgc tgcagcagca gtactattga
    22261 tgttgaccgc aggctgttcc actctggagc gagtggttta ccgtcctgac atcaaccagg
    22321 ggaactatct gaccgctaac gacgtatcca aaatacgtgt tggcatgacg caacaacaag
    22381 ttgcgtacgc attgggtaca ccgctgatgt ccgatccatt tggtacgaat acctggttct
    22441 atgtcttccg ccagcaacca ggtcatgaag gtgtaacgca gcaaacgctg acgctgacct
    22501 ttaacagtag cggtgtgttg accaatattg ataacaaacc tgcgctgagt ggtaactaat
    22561 aataaagttg ctctcaaaga cgttaaaaaa ggtgctcaat gagcaccttt tttctgtctg
    22621 ttatttattc gctgattttt ctgctcgttg cctgcgaagc tctttcggat cggcaatgag
    22681 agggcgataa atctccaccc gatcgccatc atgcacacta tcgcttagtt ttgccggacg
    22741 gctgtaaatg ccgactttgt ttttagttaa atcgatatcg gtacgcaatt ccagcaagcc
    22801 actggcgcga atagcttctt caaccgtcgc gccctcctgc agcgtcacgc gctgcaggta
    22861 ctgcttctca ggtagcgcat aagccacctc aacggcaatt ttacctggca ctgtaaacct
    22921 ctttcgcacg aaccgtaaaa gcctggacca tattagacgc cagctcttta aacacgcgac
    22981 caaaggcgag ttcaatcaac ttattggtaa actcaaagtc gagatgaaac tcgatacgac
    23041 acgcctcctg gctcagcggc gtaaacttcc atccaccaat caatttcttg aacggcccat
    23101 ccaccagatt cataagaata ctttggttac tggtcaactg gttgcgggta gtaaacgttt
    23161 tgctgatccc agccttagag acatctaccg ccgcagtcat ctgcccagga gtggactcca
    23221 gaatccgact tccggtacaa cccggcaaaa actgaggata agactgaacg tcattcacta
    23281 actgatacat ttgctccgcg ctgtagggta ccagtgcggt ccggctaatc tgaggcataa
    23341 caatttccat caacaaaaat ccaacaaata atatcattta tcctgctgta aaaaaaacgc
    23401 tatcccggcg ctgggtaaca tcgggttcat gctaagatag agccttgtcc cccgcaggat
    23461 tgatatgggg tgttttcgat ttcagattac cgatgattca cgacgcttat gacgaagaaa
    23521 aaagcacata aacctggttc agcgaccatc gcgcttaaca agcgcgcccg tcacgaatac
    23581 tttatcgaag aagagttcga agcgggactt gccctgcaag gctgggaggt taaatccctg
    23641 cgcgcaggaa aagccaatat cagcgacagc tacgtccttc tgcgtgacgg agaggcattt
    23701 ctgtttggcg ctaacatcac gccaatggcc gtggcctcca cgcatgtggt gtgcgatcct
    23761 acccgtaccc gcaagttact tctcaaccaa cgcgaactgg actcattgta cggtcgcgtc
    23821 aatcgagaag gctataccgt agtggcgctc tccctgtact ggaaaaatgc ctggtgcaaa
    23881 gtgaaaatcg gcgtcgccaa aggtaaaaaa cagcacgata aacgttcaga tatcaaagaa
    23941 cgcgaatggc aggtggataa agcacgtatc atgaaaaacg cccaccgtta aacctgcact
    24001 ccaattattg accagttcct caccgcgcct ccctctccgg cggcgcgaat gaacatctta
    24061 ttggctatca catccgacac aaatgttgcc atcccattgc ttaatcgaat aaaaatcagg
    24121 ctacatgggt gctaaatctt taacgataac gccattgagg ctggtcatgg cgctcataaa
    24181 tctggtatac ttacctttac acattggggc tgattctgga ttcgacggga tttgcgaaac
    24241 ccaaggtgca tgccgagggg cggttggcct cgtaaaaagc cgcaaaaaat agtcgcaaac
    24301 gacgaaaact acgctttagc agcttaataa cctgcttaga gccctctctc cctagcctcc
    24361 gctcttagga cggggatcaa gagaggtcaa acccaaaaga gatcgcgtgg aagccctgcc
    24421 tggggttgaa gcgttaaaac ttaatcaggc tagtttgtta gtggcgtgtc cgtccgcagc
    24481 tggcaagcga atgtaaagac tgactaagca tgtagtaccg aggatgtagg aatttcggac
    24541 gcgggttcaa ctcccgccag ctccaccaat catgattgga cggtataagg acaacaccaa
    24601 taaaaacagg aagttagcag tctcagcagg acaccgacca gacggtgaag agacataaaa
    24661 ggatacgcaa aggagccgcg gctcctggtg acatgaaagc ccacagatgt gggctttttc
    24721 gttgatggtc agaacgacca gttcacacca gctaccccgt tccacgggga ttccacaccg
    24781 gcaccatggc tataccccac cccaagatgc ccgcttaacg tactgctgaa tgaggcttta
    24841 atacctgcct ggtatattcc acgtctgccc gacaaatcat tgacgaaatt accgtcacta
    24901 ttcactttca cccggttatc atcgacaaat tctttgcgca cagccgcctt cagccacggc
    24961 tcaacttcca taccgttccc cagacgcatg ttgtaactca gcgttgcacc cagttcacga
    25021 tataaactgc gggtatcgac tgatttcgat tccatgccat tggataaatg atattcaggg
    25081 ttatcagcgg tgaacccccg ttaacgatgc atacggcgtc aggttccagt taccatcggt
    25141 aaatcgcatc ccggtttcaa tgtgaccgcc cagcccgttg ctgcgataac tgccgttggc
    25201 ggctccaccg ctgccaaccg tggagtcgcg agcttcggta cctgctaata ccaataaatt
    25261 accgccattt tccagcaaca tattggtcgc taaattgccg gaaatggaaa aagtgccgta
    25321 ctggtgagta ccgctgattt caataccgtt agccgtgctc gtctggagag cggcaccgct
    25381 gttctggacg atatctgtcg ctttgccatt atcgttaact gtcagcgtac cgccttcatt
    25441 gatctttgtt tttattgcct ctccgttagc tgaaactgtt tgtattccgc cgtcgttaat
    25501 cgttgtctca ttcgccaccc cctcgacgat ttgttcgccg ccggtgagcg tcgtgcctgt
    25561 cgcagtggct tttgttttga cgatctcccg tccgcccgta ttgacctgtg ttttgtcaga
    25621 agaggtgtct gactccatgg ttaacacgcc gccatttgcc agcaggatat tgttcgccgc
    25681 accctgctcg atgctgaacg cgacgccatc cgcgcgtgtt cctgtgaccc gcgtcgccct
    25741 ggtggttgca accaaagcgt cctggctact ctgctgtatc cccgttgcgc tgcctttctc
    25801 ccgcacatcg agtgtgccgc cgtcattaag caccgagttt tcagccagac cgccctcatt
    25861 aactacctgt gaacacccat taataatgga accttccgct gtcccgtttg ccataatttg
    25921 ttgtaggcca gagacgatat cggtattgat tgccttacca taattctgaa cggtttgcgt
    25981 gccaccattg atgtgtgttt tctctgttga cccaccatca acaatttgtt caccactttc
    26041 gatatttgcc tcagtggcta atccatatac cgtttgcttg ccacctttga tatttgcttt
    26101 atcagaagtg gcactggcat atattgtttg ggtgccagca ctattgagta cagtgccaac
    26161 atcttttcca taaacatcca ttttgccgtt ggcattaata atcgtatcaa ctgcccggga
    26221 accagtgacg actgttaatg agccagcgtt ttccagcact acatttttag cttctgaatt
    26281 cctgatgtag aaagcatcac cataactgtt ggttccttcg ataagggttc cggaagttgt
    26341 ggaagcaatt aatgcgccgc cggattgttg ctcaacatgc ttagcctcac caccgtcctg
    26401 aacctccaga acgccaccat tattaagtct ggtggtatct gttttagcct ccttctggac
    26461 aatcagctta ccgccagcat caacggtagt atttttcgcc gaggttttag ccactaccgt
    26521 cagttcgccg gtattttcca gcacaacata attagcctcc cctccggtaa tagtgaagtg
    26581 agagagcttg ttgtatcctt caatatcagt ccctgcgccc gtgttggcaa ctaaagcact
    26641 gcccgtctcc tggttaaccc catgtgcaat accaccggta tagacaatca gcgagcctcc
    26701 ggcgctaata ttgctgccag ttgccgtacc atctttctca acaacctgcc ggctcccgga
    26761 ggatataatt gtcgtgtcag ctttcccgcc gctcttgata ttttgcgttc cgccgttgat
    26821 attggtaccc gtggcaatac catgattatt aatattctgt gtaccaccat tgattatggt
    26881 attggtcgcg tttccggtaa catccattac cccgccgtta tctattcggg tcgcatcagc
    26941 tttagcattc gttaaaactg acattgttcc tttatcttta ataatcgtct tgtttgccga
    27001 accatatgcg tttatgtcta aatgaccacc gttttccagc aacacattgt ctgccacgtg
    27061 attgtggatg gagaatgcac cttcactatt cgtaccgctc accgtcgtac cgttagtgtt
    27121 tgtttttaaa attgcaccat cgtgctgggt aacatttgtt gccgtaccac cactaacatc
    27181 aagcacgcca ccggaataaa cttcaataac atccgaggag ctggtgtaat caacaatttg
    27241 cgtaccacca gaatagatct gagtattttt gccgttgact tattattcag agactgagtt
    27301 ccaccttcaa tcgtcgtgtc cagcgcatgg ctttcatata ctctttgctc accgccattt
    27361 ttaatggtgg ttgttcctac tgtgctctgt tcaacatact gtcgtccacc atttatgatt
    27421 gtgttcgttg caatacttcc ttgcgtgatg tcctgataac cggacttatt tatcgttgta
    27481 ccatcaacat gcccctgagt tgttactttc tgccctccac catcaatgat ggttccattc
    27541 gcagtccccc cacttatgtg tgaattacca cccttaattg tcgttccatt gctgatacca
    27601 cctgtataaa cgtcctgatt gccactgtcg attatcgtac cggtggaaat gcccccgtca
    27661 tgaattgtct gtcttcctcc gttaatggtt gtattattag cctgccccac aaaattgtta
    27721 tgacttccta catcttgata tccacccgat tcaataagac ttccattaga taccccgcca
    27781 tgaacattct gctggccatg gttgataata tgagtgttat ttgttgtacc tcgttcatct
    27841 actctttggc tgccatctac agtctcatcg tttaccacac caataacatc aggagtgaag
    27901 gccgtcatcc caggcggggc atatatcaag gcagatatca ataaggaaag tactgagcgg
    27961 cgacaataat ggggactggt cctgttcata aatttcatcc tctgaaaagt gaatactgag
    28021 tagcgtttaa gcgaccttag ctctgctgca acatcagccc acaggcacca gaccagggga
    28081 ttcatcctga agagacagcg caagtgtatt gtgttcaccg ctcatcaaag acatcatgat
    28141 gaaatgatga tattccacat aagaaagaag cattttttaa acgcagtgcg ctgaagtatg
    28201 gttggataaa aaagtcaatc tattcaggga atacgggggt attcttttct ttcgacaatc
    28261 aggccgtcgg caaaataaaa tgatttacat aatcgtttct gatgaatatc ttctgctcac
    28321 ataaaaataa cacaataact ttgagatcgc agattctttt acttttacag cattcgtccc
    28381 cccattgttg ggcaaatata gattgggcca gagcacgaaa gttaatacca cgtttgcaca
    28441 gctcctccaa cagcacgaca agatgccaca tactgcgccc cagtcggttc ggtttacaga
    28501 ctacccgtgc gtccaccgcc gataatgtcc tgagcagttt tttcagtccg gacctcctgc
    28561 cacggtttta cgttgaagag gtaaccctga gcacgcagtt catcagtcag gcgtggtgta
    28621 ccgtaaccct attattgatg ggtaagatca aaaaaacttt caggcagcta aggaaagttg
    28681 aaccagacat tagaataaat atttcaacca attacagcac caattcagac accgccagct
    28741 caacaaataa atcaaggggt tacgtgaaag cgtagcccct ttttctttgg tagtggcagc
    28801 aaaatggttg tagtgtaaaa aataatcccg tttaatcaat caataataca tattgtttca
    28861 atctacgtta ttatctcttt gtaaaaatag ccatttatta atcattgaaa actgctttta
    28921 gaacttgata caacgggact agtcacaaca ggactattct caacgggatc atcctcagag
    28981 gaactatcat caaagtcatc atccataaat aaaatatcat cgaatggtgc cacgcccgtg
    29041 atgagtttta ttttattatt acgatcagtc aagactccac ttaaaccgtt ttcgctcaca
    29101 ggttttaatg atttttcatt actcttgttg taagcaggcg cattaaaaat acacggagta
    29161 tcaagatcaa acaatgacgt tccccagttc acatattgaa tatcatagtt actgaagttc
    29221 tgtccagaaa agaagcatcc cttaaaatcc aatccaccta aattatataa accatcctct
    29281 tctttttgga gagcaatgtt aattttggct atctcccgga catcatcgcc atttttatat
    29341 ttgaataccg tttcaagatt ttccccacac agtttgactt ctggaaataa tttgaaatca
    29401 aagcctatat tattatgtat caacgtagat gaacaaaaga tggagaaagc ttgcagtgct
    29461 gaattatagc tttcgatttt atcctgaggc tgcgctcttg gtaaaaactc atagcaacat
    29521 tcataaaaat taagtagaaa ctctgaagac cttccatttt tatcaaataa tatccccttg
    29581 aactcattca caaacgcatc ttttttttct tgaatatcta tggggtgttc ctttgactct
    29641 gacaaagatg aaatctcatc tatttttttt catatgaatt acgtgattcc atacagacat
    29701 ttggcggcgt ttctaaaata a
//
LOCUS       DAPHDW010000047        29558 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.047, whole genome shotgun sequence.
ACCESSION   DAPHDW010000047 DAPHDW010000000
VERSION     DAPHDW010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 29558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29558
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.047"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(83..2152)
                     /gene="glyS"
                     /locus_tag="RH057_002060"
     CDS             complement(83..2152)
                     /gene="glyS"
                     /locus_tag="RH057_002060"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HDV3128426.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2162..3073)
                     /gene="glyQ"
                     /locus_tag="RH057_002061"
     CDS             complement(2162..3073)
                     /gene="glyQ"
                     /locus_tag="RH057_002061"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HDV3128427.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3168..3467)
                     /locus_tag="RH057_002062"
                     /pseudo
     CDS             complement(3168..3467)
                     /locus_tag="RH057_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3642..4637
                     /gene="wecH"
                     /locus_tag="RH057_002063"
     CDS             3642..4637
                     /gene="wecH"
                     /locus_tag="RH057_002063"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HDV3128428.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4679..5116)
                     /locus_tag="RH057_002064"
     CDS             complement(4679..5116)
                     /locus_tag="RH057_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HDV3128429.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5162..5503)
                     /locus_tag="RH057_002065"
     CDS             complement(5162..5503)
                     /locus_tag="RH057_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128430.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5672..7126)
                     /gene="xylB"
                     /locus_tag="RH057_002066"
     CDS             complement(5672..7126)
                     /gene="xylB"
                     /locus_tag="RH057_002066"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HDV3128431.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWVAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELARAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7198..8520)
                     /gene="xylA"
                     /locus_tag="RH057_002067"
     CDS             complement(7198..8520)
                     /gene="xylA"
                     /locus_tag="RH057_002067"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HDV3128432.1"
                     /translation="MQAYFDQLDRVRYEGSKSSNPLAFRHYNPDELVLGKRMEEHLRF
                     AACYWHTFCWNGADMFGVGAFNRPWQQPGEALALAKRKADVAFEFFHKLHVPFYCFHD
                     VDVSPEGASLKEYINNFAQMVDVLAAKQEESGVKLLWGTANCFTNPRYGAGAATNPDP
                     EVFSWAATQVVTAMEATHKLGGENYVLWGGREGYETLLNTDLRQEREQLGRFMQMVVE
                     HKHKIGFQGTLLIEPKPQEPTKHQYDYDAATVYGFLKQFGLEKEIKLNIEANHATLAG
                     HSFHHEIATAIALGLFGSVDANRGDAQLGWDTDQFPNSVEENALVMYEILKAGGFTTG
                     GLNFDAKVRRQSTDKYDLFYGHIGAMDTMALALKIAARMIEDGELDKRIAQRYSGWNS
                     ELGQQILKGQMSLADLAKYAQEHNLSPVHQSGRQEQLENLVNHYLFDK"
     gene            8886..9878
                     /gene="xylF"
                     /locus_tag="RH057_002068"
     CDS             8886..9878
                     /gene="xylF"
                     /locus_tag="RH057_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3128433.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKEA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTTLNNGLKDVPSRLLTPIDVNKNNIKDTVVKDGFHKESEL"
     gene            9956..11497
                     /gene="xylG"
                     /locus_tag="RH057_002069"
     CDS             9956..11497
                     /gene="xylG"
                     /locus_tag="RH057_002069"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HDV3128434.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVCLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRCQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVGRELTALYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     IAQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11475..12656
                     /gene="xylH"
                     /locus_tag="RH057_002070"
     CDS             11475..12656
                     /gene="xylH"
                     /locus_tag="RH057_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HDV3128435.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12734..13912
                     /gene="xylR"
                     /locus_tag="RH057_002071"
     CDS             12734..13912
                     /gene="xylR"
                     /locus_tag="RH057_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XylR"
                     /protein_id="HDV3128436.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDGVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLADWLQTLPPQTGIIAVTDARARHILQVCEHLHIPVPEKLCVIGI
                     DNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEEMPLQRILVPPVRVIERRS
                     TDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRSNLEKRFKEEVGETIHAMI
                     HAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKKAYDTTPKEYRDVNSEVML
                     "
     gene            complement(14020..14844)
                     /locus_tag="RH057_002072"
     CDS             complement(14020..14844)
                     /locus_tag="RH057_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HDV3128437.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAIAAERNWL
                     ISKQYQGQWSPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15164..17194
                     /gene="malS"
                     /locus_tag="RH057_002073"
     CDS             15164..17194
                     /gene="malS"
                     /locus_tag="RH057_002073"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV3128438.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDAPAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTGYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASAALREWKKANPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17372..18625
                     /gene="avtA"
                     /locus_tag="RH057_002074"
     CDS             17372..18625
                     /gene="avtA"
                     /locus_tag="RH057_002074"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HDV3128439.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEAQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEARPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18777..19250)
                     /locus_tag="RH057_002075"
     CDS             complement(18777..19250)
                     /locus_tag="RH057_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HDV3128440.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTAVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLRQQPV"
     gene            complement(19352..20200)
                     /gene="yiaJ"
                     /locus_tag="RH057_002076"
     CDS             complement(19352..20200)
                     /gene="yiaJ"
                     /locus_tag="RH057_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HDV3128441.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20401..21399
                     /gene="yiaK"
                     /locus_tag="RH057_002077"
     CDS             20401..21399
                     /gene="yiaK"
                     /locus_tag="RH057_002077"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HDV3128442.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVWAKIQAL"
     gene            21411..21878
                     /locus_tag="RH057_002078"
     CDS             21411..21878
                     /locus_tag="RH057_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV3128443.1"
                     /translation="MIFGHIAQPNPCRLPAAIEKGLDFLRATDFNALEPGVVEIDGKN
                     IYAQIIDLTTREAVENRPEVHRRYIDIQFLAWGEEKIGIAIDTGNNKVSESLLEQRDI
                     IFYHDSEHESFIEMIPGSYAIFFPQDVHRPGCILQTASEIRKIVVKVALTALN"
     gene            21996..22469
                     /gene="yiaM"
                     /locus_tag="RH057_002079"
     CDS             21996..22469
                     /gene="yiaM"
                     /locus_tag="RH057_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate TRAP transporter small
                     permease YiaM"
                     /protein_id="HDV3128444.1"
                     /translation="MKKILEAILAINLAVLSCIVFINIILRYGFQTSILSVDELSRYL
                     FVWLTFIGAIVAFMDNAHVQVTFLVEKLSPAWQRRVALVTHSLILFICGALAWGATLK
                     TIQDWSDYSPILGLPIGLMYAACLPTSLVIAFFELRHLYQLITRSNSLTSPPQGA"
     gene            22472..23749
                     /gene="yiaN"
                     /locus_tag="RH057_002080"
     CDS             22472..23749
                     /gene="yiaN"
                     /locus_tag="RH057_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter large permease
                     YiaN"
                     /protein_id="HDV3128445.1"
                     /translation="MAVLIFLGCLLGGIAIGLPIAWALLLCGAALMFWLDMFDVQIMA
                     QTLVNGADSFSLLAIPFFVLAGEIMNAGGLSKRIVDLPMKLVGHKPGGLGYVGVLAAM
                     IMASLSGSAVADTAAVAALLVPMMRSANYPVNRAAGLIASGGIIAPIIPPSIPFIIFG
                     VSSGLSISKLFMAGIAPGMMMGATLMLTWWWQASRLNLPRQQKATMQEIWHSFVSGIW
                     ALFLPVIIIGGFRSGLFTPTEAGAVAAFYALFVATVIYREMTFATLWHVLIGAAKTTS
                     VVMFLVASAQVSAWLITIAELPMMVSDLLQPLVDSPRLLFIVIMVAILIVGMVMDLTP
                     TVLILTPVLMPLVKEAGIDPIYFGVMFIINCSIGLITPPIGNVLNVISGVAKLKFDDA
                     VRGVFPYVLVLYSLLVVFVFIPDLIILPLKWIN"
     gene            23762..24748
                     /gene="yiaO"
                     /locus_tag="RH057_002081"
     CDS             23762..24748
                     /gene="yiaO"
                     /locus_tag="RH057_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diketo-L-gulonate transporter
                     substrate-binding protein YiaO"
                     /protein_id="HDV3128446.1"
                     /translation="MKLRSVTYALFIAGLAAFSTSSLAAQSLRFGYETSQTDSQHIAA
                     KKFNDLLQERTKGELKLKLFPDSTLGNAQAMISGVRGGTIDMEMSGSNNFAGLSPVMN
                     LLDVPFLFRDTAHAHKTLDGKVGDDLKASLEGKGLKVLAYWENGWRDVTNSRAPVKTP
                     ADLKGLKIRTNNSPMNIAAFKVFGANPIPMPFAEVYTGLETRTIDAQEHPINVVWSAK
                     FFEVQKYLSLTHHAYSPLLVVINKAKFDGLSPEFQQALISSAQEAGNYQRKLVAEDQQ
                     KIIDGMKEAGVEVITDLDRKAFSDALGTQVRDMFVKDVPQGADLLKAVDEVQ"
     gene            24752..26248
                     /gene="lyxK"
                     /locus_tag="RH057_002082"
     CDS             24752..26248
                     /gene="lyxK"
                     /locus_tag="RH057_002082"
                     /EC_number="2.7.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-xylulokinase"
                     /protein_id="HDV3128447.1"
                     /translation="MTQYWLGLDCGGSWLKAGLYDREGREAGVQRLPLCALSPQPGWA
                     ERDMAELWQCCMAVIRELLTHSGVSGEQIVGIGISAQGKGLFLLDKNNKPLGNAILSS
                     DRRAMEIVRRWQEDGIPEKLYPLTRQTLWTGHPVSLLRWLKEHEPERYAQIGCVMMTH
                     DYLRWCLTGVKGCEESNISESNLYNMSLGEYDPCLTDWLGIAEINHALPPVVGSAEIC
                     GEITAQTAVLTGLKAGTPVVGGLFDVVSTALCAGIEDEFTLNAVMGTWAVTSGITRGL
                     RDGEAHPYVYGRYVNDGEFIVHEASPTSSGNLEWFTAQWGEISFDEINQAVASLPKAG
                     GDLFFLPFLYGSNAGLEMTSGFYGMQAIHTRAHLLQAIYEGVVFSHMTHLNRMRERFT
                     DVHTLRVTGGPAHSDVWMQMLADVSGLRIELPQVEETGCFGAALAARVGTGVYRDFSE
                     AQRDLQHPVRTLLPDMTAHQLYQQKYQRYQHLIAALQGFHARIKEHIL"
     gene            26245..26907
                     /gene="ulaD"
                     /locus_tag="RH057_002083"
     CDS             26245..26907
                     /gene="ulaD"
                     /locus_tag="RH057_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HDV3128448.1"
                     /translation="MSRPLLQLALDHSSLEAAQRDVTQLKDSVDIVEAGTILCLNEGL
                     GAVKALREQCPDKIIVADWKVADAGETLAQQAFGAGANWMTIICAAPLATVEKGHAMA
                     QRCGGEIQIELFGNWTLDDARDWHRIGVRQAIYHHGRDAQASGQQWGEADLARMKALS
                     DIGLELSITGGITPADLPLFKDIRVKAFIAGRALAGSANPAQVAGDFHAQIDAIWGGK
                     RA"
     gene            26900..27760
                     /locus_tag="RH057_002084"
     CDS             26900..27760
                     /locus_tag="RH057_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418039.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HDV3128449.1"
                     /translation="MRNHPLGIYEKALAKDLSWPERLVLAKSCGFDFVEMSVDETDER
                     LSRLDWSTAQRTSLVAAMIETGVGIPSMCLSAHRRFPFGSRDEAVRERAREIMSKAIR
                     LARDLGIRTIQLAGYDVYYEDHDEGTRQRFAEGLAWAVEQAAASQVMLAVEIMDTAFM
                     NSISKWKKWDEMLASPWFTVYPDVGNLSAWGNDVPAELKLGIDRIAAIHLKDTQPVTG
                     QSPGQFRDVPFGEGCVDFVGIFKTLHELNYRGSFLIEMWTEKAKEPVLEIIQARRWIE
                     ARMQEAGFIC"
     gene            27754..28449
                     /gene="araD"
                     /locus_tag="RH057_002085"
     CDS             27754..28449
                     /gene="araD"
                     /locus_tag="RH057_002085"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HDV3128450.1"
                     /translation="MLEQLKADVLAANLALPAHHLVTFTWGNVSAVDDTRQWMVIKPS
                     GVEYDVMTADDMVVVEIASGKVVEGSKKPSSDTPTHLALYRRYAEIGGIVHTHSRHAT
                     IWSQAGLDLPAWGTTHADYFYGAIPCTRQMTAEEINGEYEYQTGEVIIETFEERGRSP
                     AQIPAVLVHSHGPFAWGKNAADAVHNAVVLEECAYMGLFSRQLAPQLPAMQNELLDKH
                     YLRKHGANAYYGQ"
     gene            complement(28481..28582)
                     /locus_tag="RH057_002086"
                     /pseudo
     CDS             complement(28481..28582)
                     /locus_tag="RH057_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312484.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
     gene            28586..28774
                     /locus_tag="RH057_002087"
                     /pseudo
     CDS             28586..28774
                     /locus_tag="RH057_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312486.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(28796..29536)
                     /locus_tag="RH057_002088"
     CDS             complement(28796..29536)
                     /locus_tag="RH057_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MipA/OmpV family protein"
                     /protein_id="HDV3128451.1"
                     /translation="MLINRNIVALFALPFMASATASELSIGAGAAYNESPYRGYNKNT
                     KAIPLISYEGDSFYVRQTTLGFILSQSEKNELSLTASWMPLEFDPADNDDYAMQQLDK
                     RDSTAMAGVAWYHHERWGTVKASAAADVLDNSNGWVGELSVFHKMQIGRLSLTPALGV
                     LYYDENFSDYYYGISESESRRSGLASYSAQDAWVPYVSLTAKYPIGEHVVLMASAGYS
                     ELPEEITDSPMIDRNESFTFVTGVSWRF"
BASE COUNT         7273 a         7602 c         7431 g         7252 t
ORIGIN      
        1 agaagattat taaaggttgc tgtattattt agcgataata aaaaaagcct gccagatggc
       61 aggcttttta ataacggcgt tattactgca acagcgaaat atccgcaacg cgcaggaaca
      121 gttcgcgcag tttctccagc atggtcagac ggttgagacg caattctttg tcatcaacca
      181 tgaccatcac tttatcgaag aaagcatcaa ccggctcacg cagctctgcc agttcgacca
      241 gcgcatcctg gtaacgacct tccgcaaagt acggctccag cttgtcacgt agcaccacaa
      301 cctgcatcgc cagtttaatt tcttccggct ctttcagggt agaggcattc acgcggtcgc
      361 tcagcacttc gtcagatttc gccaaaatgt tggagacacg cttgttcgcc gccgccagtg
      421 cagcagctgc ttcaagggta cggaagtgcg ataccgcttt catgcgggca tcgaaatcag
      481 ccggacgagt cggacgacgc gccagtaccg cctggatggt gtcaacggtg taaccttcgt
      541 cctgatacca ggcgcggaag cgaccgagca taaagtcgat aacatcatca accacgttgg
      601 cgttagtcag cttatcgcca tacagacgca ctgcttcttc ggtcagcgtt tgcagatcaa
      661 ggttgaggtt cttctcaacg ataattcgca gcacgccaag tgcggcacga cgcagcgcaa
      721 acgggtcttt gtcgcctttc ggatgctggc cgataccgaa gatacccgcc agagtatcca
      781 tcttgtcagc aatcgccagc gcacaggcta ccgggttaga cggcaggtca tcaccggcaa
      841 agcgcggctg atactgctca ttcagcgcca ccgcgacatc ttccgcttcg ccatcgtgac
      901 gcgcatagtg catccccata acgccctggg tgtcggtgaa ctcgaagacc atgttggtca
      961 tcaggtcaca cttagacagc aggcccgcac gggttgcgtg gttaacgtca gcgccaatct
     1021 gttcagcaat ccagccagcc agcgcctgga tgcggtcagt tttgtcacgc agcgtaccca
     1081 gctgttgctg gaacagcacg gtttgcaggc gcggcaggtt atcttccaga cgttttttac
     1141 ggtcggtgtt gaagaagaac tcggcatccg ccagacgcgg acgaacgact ttctcgttac
     1201 cggagataat ctgctgcgga tctttcgatt cgatgttggc aacgaagata aagttcggca
     1261 gcagtttgcc gtcgttcgca tacaccggga agtatttctg gtcacctttc atggtgtaaa
     1321 ccagcgcttc agccggcacc gcgaggaatt tctcttcgaa ttttgcggtc agaacgaccg
     1381 gccactccac cagcgaagcc acttcttcca gcaggctttc gcttaagtca gcgttaccgc
     1441 caatcttacg cgctgcttct tcggcatcgg ctttaatctt cgctttacgt tcttcgtaat
     1501 cggcgatgac tttcccgcgc tcacgcagaa tttccggata ctgatcggcg ttgtcgatgg
     1561 tgaattccgg ttcgcccata aagcggtggc cgcgaatcac gcgatcggac tgaatgccca
     1621 gaatggttgc cggaatgact ttgtcgccca gcagcagggt cacggtgtgc accggacgca
     1681 cgaagtgcac gtcgcttgcg ccccaacgca tcagtttcgg gatcggcagt ttcgccagag
     1741 aagtcgcaac catattcggc agcagtgctt cggtgctttc gcccttcaca tgggcgcgat
     1801 acagcagcca ttcgccttta tcggtagtca gacgctcagc ctggtcaacg gtaataccgc
     1861 aaccacgcgc ccaaccttct gccgctttgc tcggtttgcc ttcagcgtcg aacgcctggg
     1921 caatcgccgg gccgcgtttt tcgatttcac gatccggttg cgcttccgcc aggttagcta
     1981 ctttcagcgc cagacgacgc ggagcagcaa accattgaac ggtgccgtgt gcgaggccag
     2041 cgttatccag ctccgcagta aaattcgcag caaaggactc agccaggctg cgcagtgctt
     2101 ttggtggcag ctcttcagtg ccgatttcca ccagaaaagt tttctcagac atagccgcct
     2161 cttacttatc tttgttgcac atcgggaagc cgagggcttc acgggaagcg tagtatgctt
     2221 ctgccactgc tttggtcagg gtgcgaatgc gcagaatgta gcgctgacgc tcggtgacgg
     2281 agatggcttt acgcgcatcc agcaggttga agctgtgggc ggctttcaga atacgctcgt
     2341 aggctggcag cggcagcgga ttttccagcg ccagcagctg ctgcgcttct ttctcgtact
     2401 gctcaaagca ggtgaacagg aagtccacat ccgcgtattc gaagttgtaa gtggactgct
     2461 ccacttcgtt ctgatggaac acgtcgccgt aggtggtttt acccagcggg ccgtcgctcc
     2521 agaccaggtc gtaaacgctg tctacgccct gaatgtacat ggccagacgt tccagaccgt
     2581 aggtgatctc gccggtaacc ggtttacact ccagaccacc aacctgctgg aagtaagtga
     2641 actgcgtcac ttccatgccg tttagccaca cttcccagcc cagtccccag gcacccagag
     2701 tcgggttttc ccagttatct tccacgaaac ggatgtcgtg aatagtcggg tccatgccca
     2761 gctctttcag agaaccgagg tacagctcct gaatattgtc cggcgatggc ttaatgacca
     2821 cctgaaactg atagtagtgc tgtaaacggt tggggttttc gccgtagcga ccatcggtcg
     2881 ggcgacgaga aggctgaaca taagcagccg ccatcggttc tggccccagc gcgcgcagac
     2941 aggtcattgg gtgagaggtt cccgcgccga cttccatgtc caatggttga acaatggtgc
     3001 agccctggcg agcccagtaa tcctgtaagg tcaggatcaa gccctggaag gtcctggtat
     3061 caaacttttg catattattt cgtgctggat acgtgtggat ttaaaggaag ggatcagtat
     3121 acccgctgga tggaagatat acagtacgaa acgggaaaaa gcagggctta acgcatggaa
     3181 agatgcaaaa aatggccatc tgcgtcaaaa gaacagacaa actgctcttt acggaaggta
     3241 taaccgcgca tttcatagct tccctggaac tgttcgaagg cggtgacgtc gattttttgc
     3301 tcgccggtgt tataacgttt tgccgcctaa tccttgcata acgcttccat attcaacgaa
     3361 cgcagaggat caacctttac ccgttgtgct ttctgcacgg gtgacggtgt agaacaaccc
     3421 gctagcacca taagcgccat tgccggaaag aaagcgttca tcatcattaa attaccgtct
     3481 ggtttgcagg tgagtcttat tatttatatc ggtatagtgg ctgatatctt acgaatttcc
     3541 ttaagcctct ataagctgta gtgataaaac tcagattatt ccagtgcgcc aactgaatga
     3601 tttatcggtc agactttttt taaccagata cagagacaca gatgcagccc aaaatttact
     3661 ggattgataa cctgcgaggg atagcgtgtt taatggtggt gatgattcac accactacct
     3721 ggtatgtgac caatgctcat agtgttagcc ccgtcacctg ggatatcgcc aatgttctga
     3781 attctgcctc tcgtgtcagc gtgccgctat ttttcatgat atccggctat ctcttttttg
     3841 gcgaacgcag cgcccagccg cgccatttct tgcgtatcgg cttatgcctg attttttata
     3901 gcgcaatcgc actgctctac attgcgctgt ttacctccat caatatggag ttagcgctga
     3961 aaaacctgct gcaaaagcca gtgttttacc acttgtggtt tttcttcgcg attgcggtga
     4021 tttatctggt ttcaccgctg attcaggtga agaacgtcgg cggaaaaatg ttgctggtgc
     4081 taatggtggt gattggcatt atcgctaacc caaacacagt gccgcagaaa attgacggtt
     4141 ttgaatggct gccaattaac ttatatatca atggcgatac tttttactac attctgtatg
     4201 gcatgttggg ccgcgctata gggatgatgg acacacagca taaagcactg tcgtgggtga
     4261 gcgccgcgct gtttgcgacg ggggttttta ttatctctcg cgggacatta tatgaattgc
     4321 agtggcgcgg aaattttgcc gatacctggt atctttactg tgggccgatg gtttttatct
     4381 gcgcaatcgc gctattgact ctggttaaaa acacgctgga tacgcgtacc attcgcggac
     4441 ttggcttaat ctcccgccat tcattgggta tatacggatt ccacgccttg attatccatg
     4501 cgctgcgcac ccggggaatt gagcttaaaa attggccaat actggatatt atttggatct
     4561 tttgcgcgac gttggcagcg agtttgttac tttctatgct ggtacaacga atcgacagaa
     4621 acagattagt gagttaagta aaagcccgtt cacattggac tgaccgggct tacgtgaatt
     4681 attcgctgta ttcttcctgg gtaacctgtg tttcttttgg cgggtttatt ccggcatcac
     4741 gaatattctt ctgcaccgct actgcaccaa acaagcttaa gaagaaagcc agtccataaa
     4801 aacctttttc actgagtaat aatgtcgcat tccacagacc taccatcagt aatgcaacag
     4861 agataataaa gacagtcagg caggtcatat aataaatgga agtggtcggt atgccttcat
     4921 acttgtcccg aacggtcttt tgataagacg ctgcagaaaa cagtcctaac accagtacgg
     4981 caaaataata ccctttttca tttaactgca tctctgcatt ccatagccct aacagatagg
     5041 taacgatacc accaacgaga gctatccatg acacaatact aaaggccggt gaataggttg
     5101 atattttgtt gtccatcaca gtattccttt cattcctgaa tgtgtaagag ctttatgttg
     5161 ctcataccga gcgaaacaaa cgcatttgac caaacaagca gacaaaaaag gcgatggggt
     5221 agatcaccat ttcataagca ttgataggtg catttaaaac accgataaac aagagaccga
     5281 tagtgattaa cgcaactaac tggcagatat gggtaaaaaa attatccagt tgcccgagtt
     5341 tttctgcgcg aagatatgca tagttaccaa aagttgctgt cattaacacg ccaagaaaat
     5401 agccttttcc acttagcaat gggcatgaga tccatagccc aaccagataa accagcgccc
     5461 cgacaacaaa taatagtttt gccacagttt ttgaggtttt cataacgatc tccatatcta
     5521 ccagcgatac attacgagta accaacgaaa gacaaaactg aaaaatgcca ttaacaaatg
     5581 attttcagaa taaattcata ctaaatatta attaattagt gagatatata gatgtgaatt
     5641 atcccccacc cggtcaggca ggggataacg tttacgccat taatggcaga agttgctgat
     5701 agaggcgacg gaacgtttca cgtcgtggct gataagcggc ataacgctgc gcatctggta
     5761 gatgcgactg ttctaacggt agctgcggca acaactcaat gagcgatttc tctggattcg
     5821 ccgcaatctg cgccagcctt gctgcgccca gtgctggccc cacatccccc cccgtacggt
     5881 aatcgagctg ctgaccgctg atatccgcca gcatctgacg ccagtactca ctacgcgccc
     5941 cgccaccaat caacgtaaca ctttgcggtt taatgccgca ggcatgcacg acatccatgc
     6001 catctgccag cgcataaccc acgccttcca gcactgctcg cgccagttca ttggggccat
     6061 gttgatgagt caaaccaaag aaaaccccct tcgcctgggg attattgtgt ggtgtacgct
     6121 cgccggaaag ataaggcaga aaccaaactg gctcggcact ttcatcagcc tgttgagctg
     6181 cagcgattaa agctgggaca ttgcacaggc cggttaattt cgcgacccaa tccagacacg
     6241 atgctgcact cagcattaca gacattaaat gccaacgctg cggtagcgca tggcaaaagc
     6301 tgtgtacagc gctttctggc ttgcttaaga acccttcgct gacagcaaaa tagacccccg
     6361 acgtccccag cgataacatt gcctgattag catcaaccat tcccacacca actgcaccag
     6421 ctgcattgtc gccaccgcct gcaacaactg gcaccgtcgc cataccccac gctttcgcaa
     6481 cttcaggtaa caaagcacca gtaatttcgc tgccttcgta taatgcgggc atctggtcac
     6541 gagataagtc gcaagcctgc agcatgacgt cactccagtc acgctttgcg acatccagcc
     6601 acatggtgcc agctgcgtca gacatatcgc tggcaaactc ccccgtcata cgcagacgca
     6661 agtaatcttt cggtaataat actttgtcga tttgacggaa tatctccggc tcatgccgct
     6721 gaacccatag caatttaggc gcagtaaatc cgggcatcat caggttgccg gtaatcactc
     6781 gtgattgcgg aactctcgct tccagcaaag tgcactcttg cgcacagcgc ccgtcgttcc
     6841 acaaaatggc agggcgcaat acccgttgtt gagcatccag taaggttgct ccatgcatct
     6901 ggccggcaat acccaatgct ttaacgtcct gcagagaatg ctgatcgccc agagctttca
     6961 ttgcgcgatc agttgcctgc caccactgtt ccgggtcttg ttccgaccag agtggatgcg
     7021 ggcgcgaaac ggtcagcttt tccgtttgcg aagcaaccac ctcaccctgc tcgttgagca
     7081 aaataacttt tacgcccgag gtgccaagat ctatcccgat atacatatcg atcgttcctt
     7141 aaaaaaatgc ccggtatcgc taccgataac cgggccaacg gactgcacag ttagccgtta
     7201 tttgtcgaac agataatgat ttaccagatt ttccagttgc tcctggcgac cactctgatg
     7261 caccggagac aaattatgtt cctgagcata tttggctaaa tctgccagtg acatttggcc
     7321 tttcaggatt tgctggccca attcgctatt ccagccggaa taacgctgcg cgatgcgttt
     7381 atccagctcg ccatcttcaa tcatgcgcgc tgcaattttc agcgccagtg ccatcgtatc
     7441 catcgcgccg atatgaccgt aaaacagatc atatttatca gtactttgac gacgtacttt
     7501 ggcatcgaag ttcagaccac cggtggtgaa accgcctgct ttgagaattt catacatcac
     7561 cagcgcattc tcttccacac tgttcgggaa ctggtcggtg tcccagccca gttgcgcatc
     7621 gccacggttg gcgtcgacag aaccgaacag gccaagcgca atggcggtgg ctatttcatg
     7681 atggaaagag tgacctgcca gcgtcgcgtg gttagcttca atgttcagtt taatctcttt
     7741 ttccagacca aactgtttca ggaagccata gaccgtcgcg gcatcgtaat catattgatg
     7801 tttagtcggt tcttgcggtt tcggttcgat aagcaacgtg ccctggaagc cgattttatg
     7861 tttatgctca accaccatct gcataaagcg gcccagttgt tcacgctcct gacgcaagtc
     7921 ggtatttaac agcgtttcgt aaccttcacg accaccccac aggacatagt tttcaccgcc
     7981 caatttatgg gttgcttcca tcgctgtaac aacttgcgtt gccgcccagc tgaagacttc
     8041 aggatctggg ttcgtcgccg cacccgcgcc atagcgaggg tttgtaaagc agttagcggt
     8101 tccccacagc agcttcacgc cgctctcttc ttgcttggct gccaggacat caaccatttg
     8161 cgcaaaatta ttgatgtact cttttaacga cgcgccctca ggggaaacat ccacatcgtg
     8221 gaagcaataa aatggcacat gtaacttgtg gaaaaactca aatgcgacat ctgctttacg
     8281 cttcgccaac gccagtgcct caccaggctg ctgccacgga cgattaaacg cccccacacc
     8341 aaacatgtcc gccccgttcc agcagaaggt gtgccagtag caggcggcaa aacgcaagtg
     8401 ctcttccata cgcttgccca acaccagttc gtcgggattg tagtgacgga atgctaacgg
     8461 gtttgaggat tttgagcctt cataacgaac gcgatcgagc tggtcaaaat aggcttgcat
     8521 attgaactcc ataatcaggt aataccgcgg gtgatggatg atgtcgtaat attgggcact
     8581 ccctttcagt tgctcaatta tgttatttca cactgctatt gagataattc acaagtgtgc
     8641 gctcgctcgc aaaataaaat ggaatgatga aactgggtaa ttcctcgaag agaaaaatgc
     8701 aataagtaca attgcgcaac aaaagtaaga tctcggtcat aaatcaagaa ataaaccaaa
     8761 aatcgtaatc gaaagataaa aatctgtaat tgttttcccc tgtttagtcg ctaaaaattg
     8821 gttacgttta tcgcggtgat tgttacttat taaagctgtc ctctaactac agaaggccct
     8881 acaccatgaa aataaagaac attctactca ccctttgcac ctcacttctg cttaccaacg
     8941 ttgcggcaca cgccaaagaa gtcaaaatag gtatggcgat tgatgatctc cgtcttgaac
     9001 gctggcaaaa agatcgagat atttttgtga aaaaggcaga atctctcggc gcgaaagtat
     9061 ttgtacagtc tgcaaatggc aatgaagaaa cacaaatgtc gcagattgaa aacatgataa
     9121 accggggtgt cgatgttctt gtcattattc cgtataacgg tcaggtatta agtaacgttg
     9181 taaaagaagc caaacaagaa ggtattaaag tattagctta cgatcgtatg attaacgatg
     9241 cggatatcga tttttatatt tctttcgata acgaaaaagt cggtgaactg caggcaaaag
     9301 ccctggtcga tattgttccg caaggtaatt acttcctgat gggcggctcg ccggtagata
     9361 acaacgccaa gctgttccgc gccggacaaa tgaaagtgtt aaaaccttac gttgattccg
     9421 gaaaaattaa agtcgttggt gaccaatggg ttgatggctg gttaccggaa aacgcattga
     9481 aaattatgga aaacgcgcta accgccaata ataacaaaat tgatgctgta gttgcctcaa
     9541 acgatgccac cgcaggtggg gcaattcagg cattaagcgc gcaaggttta tcagggaaag
     9601 tagcaatttc cggccaggat gcggatctcg caggtattaa acgtattgct gccggtacgc
     9661 aaactatgac ggtgtataaa cctattacgt tgttggcaaa tactgccgca gaaattgccg
     9721 ttgagttggg caatggtcag gaaccaaaag cagataccac actgaataat ggcctgaaag
     9781 atgtgccctc ccgcctcctg acaccgatcg atgtgaataa aaacaacatc aaagacacgg
     9841 tagttaaaga cggattccac aaagagagcg agctgtaagc gttacgcccc ggcgcggagc
     9901 gggggcgtga tttctctcca tgccgcgtga atgaattggc ttaggtggag tcgttatgcc
     9961 ttatctactt gaaatgaaga acattaccaa aaccttcggc agtgtgaagg cgattgataa
    10021 cgtctgcttg cggttgaatg ctggcgaaat cgtctcactt tgtggggaaa atgggtctgg
    10081 taaatcaacg ctgatgaaag tgctgtgtgg tatttatccc catggctcct acgaaggcga
    10141 aattattttt gcgggagaag agattcaggc gagtcacatc cgcgataccg aacgcaaagg
    10201 tatcgccatc attcatcagg aattggccct ggtgaaagaa ttgaccgtgc tggaaaatat
    10261 cttcctgggt aacgaaataa cccacaatgg cattatggat tatgacctga tgacgctacg
    10321 ctgtcagaag ctgctcgcac aggtcagttt atccatttca cctgataccc gcgttggcga
    10381 tttagggctt gggcaacaac aactggttga aattgccaag gcacttaata aacaggtgcg
    10441 cttgttaatt ctcgatgaac cgacagcctc attaactgag caggaaacgt cggttttact
    10501 ggatattatt cgcgatctac aacagcacgg tatcgcctgt atttatattt cgcacaaact
    10561 caacgaagtc aaagcgattt ccgatacgat ttgcgttatt cgcgacggac agcacattgg
    10621 tacgcgtgat gctgccggaa tgagtgaaga cgatattatc accatgatgg tcgggcgaga
    10681 gttaaccgcg ctttacccta atgaaccaca taccaccgga gatgaaatat tacgtattga
    10741 acatctgacg gcatggcatc cggtcaatcg tcatattaaa cgagttaatg atgtctcgtt
    10801 ttccctgaaa cgtggcgaaa tactgggtat tgccggactc gttggtgccg gacgtaccga
    10861 gaccattcag tgcctgtttg gcgtgtggcc cggacaatgg gaaggaaaaa tttatattga
    10921 tggcaaacag gtagatattc gtaactgtca gcaagccatc gcccagggga ttgcgatggt
    10981 acccgaagac agaaagcgcg acggcatcgt tccggtaatg gcggttggta aaaatattac
    11041 cctcgccgca ctcaataaat ttaccggtgg cattagccag cttgatgacg cggcagagca
    11101 aaaatgtatt ctggaatcaa ttcagcaact caaagttaaa acgtcgtccc ccgaccttgc
    11161 tattggacgt ttgagcggcg gcaatcagca aaaagcgatc ctcgctcgct gtctgttact
    11221 taacccgcgc atcctcattc ttgatgaacc taccaggggt atcgatattg gcgcgaaata
    11281 cgagatctac aaattaatta accaactcgt ccagcagggt attgccgtta ttgtcatctc
    11341 ttccgaatta cctgaagtgc tcggccttag cgatcgggta ctggtgatgc atgaagggaa
    11401 actaaaagcc aacctgataa atcataacct gactcaggag caggtgatgg aagccgcatt
    11461 gaggagcgaa catcatgtcg aaaagcaatc cgtctgaagt gaaattggcc gtaccgacat
    11521 ccggtggctt ttccgggctg aaatcactga atttgcaggt cttcgtgatg attgcagcta
    11581 tcatcgcaat catgctgttc tttacctgga ccaccgatgg tgcctactta agcgcccgta
    11641 acgtctccaa cctgttacgc cagaccgcga ttaccggcat cctcgcggta ggaatggtgt
    11701 tcgtcataat ttctgctgaa atcgaccttt ccgtcggctc aatgatgggg ctattaggtg
    11761 gcgtcgcggc gatttgtgac gtctggttag gctggccttt gccacttacc atcattgtga
    11821 cgttggttct gggactgctt ctcggtgcct ggaacggatg gtgggtcgcg taccgcaaag
    11881 tcccttcatt tattgtcacc ctcgcgggca tgttggcatt tcgcggcata ctcattggca
    11941 tcaccaacgg cacgactgtt tcccccacca gcgccgcgat gtcacaaatt gggcaaagct
    12001 atctacctgc cagcactggc ttcatcattg gcgcgcttgg cttaatggct tttgttggtt
    12061 ggcaatggcg cggaagaatg cgccgtcagg ctttgggttt gcagtctccg gcctctaccg
    12121 cagtagtcgg tcgccaggct ttaaccgcta tcatcgtatt aggcgcaatc tggctgttga
    12181 atgattaccg tggcgttccc actcctgttc tgctgctgac gttgctgtta ctcggcggaa
    12241 tgtttatggc aacgcggacg gcatttggac gacgcattta tgccatcggc ggcaatctgg
    12301 aagcagcacg tctctccggg attaacgttg aacgcaccaa acttgccgtg ttcgcgatta
    12361 acggattaat ggtagccatc gccggattaa tccttagttc tcgacttggc gctggttcac
    12421 cttctgcggg aaatatcgcc gaactggacg caattgcagc atgcgtgatt ggcggcacca
    12481 gcctggctgg cggagtggga agcgttgccg gagcagtaat gggggcattt atcatggctt
    12541 cactggataa cggcatgagt atgatggatg taccgacctt ctggcagtat atcgttaaag
    12601 gtgcgattct gttgctggca gtatggatgg actccgcaac caaacgccgt tcttgatttt
    12661 gataaaaatt ttctcaaagc cggttacgta ttaccggttt tgagtttttg catgattcag
    12721 caggaaaaga accatgttta ctaaacgtca ccgcatcaca ttactgttca atgccaataa
    12781 agcctatgac cggcaggtag tagaaggcgt aggggaatat ttacaggcgt cacaatcgga
    12841 atgggatatt ttcattgaag aagatttccg cgcccgcatt gataaaatca aggactggtt
    12901 aggagatggc gtcattgccg acttcgacga caaacagatc gagcaagcgc tggctgatgt
    12961 cgacgtcccc attgttgggg ttggcggctc gtatcacctt gcagaaagtt acccacccgt
    13021 tcattacatt gccaccgata actatgcgct ggttgaaagc gcatttttgc atttaaaaga
    13081 gaaaggcgtt aaccgctttg ctttttatgg tcttccggaa tcaagcggca aacgttgggc
    13141 cactgagcgc gaatatgcat ttcgtcagct tgtcgctgaa gaaaagtatc gcggagtggt
    13201 ttatcagggg ttagaaaccg cgccagagaa ctggcaacac gcgcaaaatc ggctggcaga
    13261 ctggctacaa acgctgccac cgcaaaccgg gattattgcc gttactgacg cccgagcgcg
    13321 gcatattctg caagtatgtg aacatctaca tattcccgta ccggaaaaat tatgcgtgat
    13381 tggcatcgat aacgaagaac tgacccgcta tctgtcgcgt gtcgcccttt cttcggtcgc
    13441 tcagggcgcg cggcaaatgg gctatcaggc ggcaaaactg ttgcatcgat tattagataa
    13501 agaagaaatg ccgctacagc gaattttggt cccaccagtt cgcgtcattg aacggcgctc
    13561 aacagattat cgctcgctga ccgatcccgc cgttattcag gccatgcatt acattcgtaa
    13621 tcacgcctgt aaagggatta aagtggatca ggtactggat gcggtcggga tctcgcgctc
    13681 caatcttgag aagcgtttta aagaagaggt gggtgaaacc atccatgcca tgattcatgc
    13741 cgagaagctg gagaaagcgc gcagtctgct gatttcaacc accttgtcga tcaatgagat
    13801 atcgcaaatg tgcggttatc catcgctgca atatttctac tctgttttta aaaaagcata
    13861 tgacacaacg ccaaaagagt atcgcgatgt aaatagcgag gtcatgttgt agcgatgatg
    13921 agaattgtcg gcgtcacatc agggaactca gcacagccac ctggtgcgag caaaagcctt
    13981 atccgaccga caaatcagat aaggctcaag gaaatgcaat tacatatgcg ccgcgattaa
    14041 ccgttggtta tcctggtaca ttgcgaacag gtagttgtta taactcttcc ctttggtcga
    14101 gtagcccttc agcttgtgaa tcatcgctgt ggcagtcact tcctgatccg ctttacgcag
    14161 ctgcgcacgc gatttacgga acgaagagta agccgggtgc gtattcaggt tagtgacata
    14221 ggcgctcacc gattctttga cagaactaaa ctgtgagtac cctttcactt tacctggcgc
    14281 attggtacaa cgtcctttca tgcatttcat gccgaacagg ttgttgttgt tgcgcgccag
    14341 cttcgacgtt ccccaaccgc tttctgctgc agccatcgtc gccaccatac tggtggggat
    14401 aatgtctacg cgttcaagca aggtattcca cgggattttt cgcgtattac cagaccactt
    14461 caccttgtag cgtttggcga tgtctttcag acgcgcacgc tcagcaggtg accattggcc
    14521 ctgatactgt tttgaaatca gccagttacg ttctgcagca atggctgcat tttggctggt
    14581 aatgtaaggc attacggtcc ggagaaacgc ctttttcctt ggtgttccgg aagggtattt
    14641 tcgcaaatca ggaagtgaac tactctttgc actattgcga gaatactctt gtttactgct
    14701 tacctgttta ttactagctt tagttaagtg ggacttttga ctcgctgttg ttgtgtgcgt
    14761 cttcgctaac acctcactcg aaaacaccag agtgagtaac ataagaatca tcgccccata
    14821 tcgtcgtatg ggagtcaaaa tcatcaggtc tcctggtcgg atttaatcat tccaacacct
    14881 tatatttttc acaaatttga gagttgaatc tcaaatcata tcaaaaatag ctgtcaagag
    14941 caccccaagg aatagtccaa atctgaaact atgtcacgtg ttaacgatta agattggcgc
    15001 taaattgcag aaaatgtggg gtttatcgca aaattcagcc gttttttgcg cgagatcgct
    15061 cacccttgct tctcatcctg tggacttacc gctcagggat gagttttgtc tggcttatcg
    15121 ctggcaaact gtctgaaatc gcagcaataa ggactcatcc accatgaaac tcgccgcctg
    15181 ttttctgaca ctccttcctg gcttcgccgt tgccgccagc tggacttctc cggggttccc
    15241 tgcctttagc gaacagggaa cgggaacatt tgtcagccac gcgcagttgc ccaaaggtac
    15301 gcgtccactc acgctaaatt ttgaccaaca gtgctggcag cctgcagatg cgataaaact
    15361 caatcagatg ctttccctgc aaccttgtag caacacgccg cctcaatggc gattgttcag
    15421 agacggcaaa tatacgctgc aaatagacac ccgctccggt acgccaacat tgatgatttc
    15481 catccagaac gccgccgaac cggtagcaaa cctggtccgt gaatgcccaa aatgggatgg
    15541 attaccgctc acgctggatg tcagcgccac tttcccggaa ggagccgccg tacgggatta
    15601 ttacagccag caaattgcga tagtgaagaa cggtcaaata acgttacaac ccgctgctac
    15661 cagcaacggt ttactcctgc tggaacgggc agaaactgac gcccctgccc ctttcgactg
    15721 gcataacgcc acggtttact ttgtgctgac agatcgtttc gaaaacggcg atcccagtaa
    15781 tgaccagagt tacggacgtc ataaagacgg tatggcggaa attggcactt ttcacggcgg
    15841 cgatttacgc ggcctgacca acaaactgga ttacctccag cagttgggcg ttaatgcttt
    15901 atggataagc gccccatttg agcaaattca cggctgggtc ggcggcggta caaaaggcga
    15961 tttcccgcat tatgcctacc acggttatta cacacaggac tggacgaatc ttgatgccaa
    16021 tatgggcaac gaagccgatc tacggacgct ggttgatagc gcacatcagc gcggtattcg
    16081 tattctcttt gatgtcgtga tgaaccacac cggctatgcc acgctggcgg atatgcagga
    16141 gtatcagttt ggcgcgttat atctttctgg tgacgaggtg aaaaaaacgc tgggtgaacg
    16201 ctggagcgac tggaaacctg ccgccgggca aacctggcat agctttaacg attacattaa
    16261 tttcagcgac aaaacaggct gggataaatg gtggggaaaa aactggatca gaacggatat
    16321 cggcgattac gacaatcctg gattcgacga tctcactatg tcgctggcct ttttgccgga
    16381 tatcaaaacc gaatcaacta ccgcttctgg tctgccggtg ttctataaaa acaaaacgga
    16441 tactcacgct aaagtcatcg aaggctttac acctcgcgat tacttaaccc actggttaag
    16501 tcagtgggtc cgcgactatg ggattgatgg ttttcgggtc gataccgcca aacatgttga
    16561 gttgcccgct tggcagcaac tgaaaaccga agccagcgcc gcgcttcgcg aatggaaaaa
    16621 agctaacccc gacaaagcat tagatgacaa acctttctgg atgaccggtg aagcctgggg
    16681 ccacggcgtg atgcaaagtg actactatcg ccacggcttc gatgcgatga tcaatttcga
    16741 ttatcaggag caggcggcga aagcagtcga ttgtctggcg cagatggata cgacctggca
    16801 gcaaatggcg gagaaattgc agggtttcaa cgtgttgagc tacctctcgt cgcatgatac
    16861 ccgtctgttc cgtgaagggg gcgacaaagc agcagagtta ttactattag cgccaggcgc
    16921 ggtacaaatc ttttatggcg atgaatcctc gcgtccgttc ggtcctacag gttctgatcc
    16981 gctgcaaggt acacgttcgg atatgaactg gcaggatgtt agcggtaaat ctgccgccaa
    17041 cgtcgcgcac tggcagaaaa tcagccagtt ccgcgcccgc catcccgcaa ttggcgcggg
    17101 caaacaaacg acactttcgc tgaagcaggg ctacggcttt gttcgtgagc atggcgacga
    17161 taaagtgctg gtcatctggg ctgggcaaca gtgacttttc cggcttcccg ttcgtcagta
    17221 cctcgggaag ccgccaacca ggataaaatg tcagccctaa tcagcgttgc aggataaagc
    17281 accgctcact ctccaacaga ccgatttgca ccccagcaaa tgtagcgtta ttgttacctt
    17341 ccttgctaca gagttcgaca gatatcccgc tatgacattc tccctttttg gtgacaaatt
    17401 tacccgccac tccggcatta cgctgctgat ggaagatctg aacgacggtt tacgcacgcc
    17461 tggcgcgatt atgctcggcg gcggtaatcc ggcgcagatc ccggaaatgc aggactactt
    17521 ccagacgcta ctgaccgaca tgctggaaag tggcaaagcg actgatgcac tgtgcaatta
    17581 cgacggtcca caggggaaaa cggagctact cacactgctt gccggaatgc tgcgcgagaa
    17641 gttgggttgg gatatcgaag cacagaatat tgcactaaca aacggcagcc agagcgcgtt
    17701 tttctactta tttaatctgt ttgccggacg ccgtgccgat ggtcgggtca aaaaagtgct
    17761 gttcccgctg gcaccggaat acattggcta cgctgacgcc ggactggaag aagatctgtt
    17821 tgtctctgcg cgtccgaata ttgaactgct gccggaaggc cagtttaaat accacgtcga
    17881 ttttgagcat ctgcatattg gcgaagaaac cgggatgatt tgcgtctccc ggccgacgaa
    17941 tccaacaggc aatgtgatta ctgacgaaga gttgctgaag cttgacgcgc tggcgaatca
    18001 acacggcatt ccgctggtga ttgataacgc ttatggcgtc ccgttcccgg gtatcatctt
    18061 cagtgaagcg cgcccgctat ggaatccgaa tatcgtgctg tgcatgagtc tttccaagct
    18121 ggggctgcct ggctcccgct gcggcattat catcgccaat gaaaaaatca tcaccgccat
    18181 caccaatatg aacggcatta tcagcctggc acctggcggt attggtccgg cgatgatgtg
    18241 tgaaatgatt aagcgtaacg atctgctgcg cctgtctgaa acagtcatca aaccgtttta
    18301 ctaccagcgt gttcaggaaa ctatcgccat cattcgccgc tatttaccgg aaaatcgctg
    18361 cctgattcat aaaccggaag gagccatttt cctctggcta tggtttaagg atttgcccat
    18421 tacgaccgag cagctctatc agcgcctgaa agcacgcggc gtgctgatgg tgccggggca
    18481 caacttcttc ccagggctgg ataaaccgtg gccgcatacg catcaatgta tgcgcatgaa
    18541 ctacgtacca gagccggaga aaattgaggc gggggtgaag attctggcgg aagagataga
    18601 aagagcctgg gctgaaagtc actaaaaatt gcctgatgcg ctacgcttat caggcctaca
    18661 tgatttctgc aatatattga atttggaaga atttgtaggc cggataaggc gtttacgccg
    18721 catctggcat aaacaaagcg cactattccg gcgttgagaa acgccggaaa aacgtttcaa
    18781 acaggctgct gccgtagccg atgccgctgc actttctcga catccatgca ctgcaacgcc
    18841 tttgtcgggc aggcttcaac acaggccggt cccgtctccc gatgccagca cagatcgcac
    18901 ttaatcgccc tcgccttttt gcgcgaagaa acgacctcca tcgcaccaaa cgggcaagcc
    18961 agcatacagc ttttacagcc aatgcaacgt gtttgttcaa cgaaaatatg cccatgttcg
    19021 cggcttatcg cgtcaacagg gcagacattc gcgcacggtg catcttcaca ctgatgacag
    19081 gctactgccg tggtccagct gtggtcttta atgacacgaa tacgggaaat aaactcgtct
    19141 ggtgacaacg cagcgcaatc ctgattctcc tgatgcgaca ctgcgcaagc cacttcacag
    19201 gtacggcaac cgatacattt cgtcgcatcc gcaataataa accggttcat gatcactcct
    19261 ttgcactaaa acagcagagt gccagagagc gcgtccctac tgctttgatc caacgaagga
    19321 aatgttaaag ttttggcact tgtccaaagc gttatgtgat cgcgcccagg tcatccctga
    19381 cagtaaatcc cagttcatta gaaatagcct gcgcggtttc acgcagtggt ttcaggagat
    19441 ttttctctcc cacctgtttc agacgtgatg tcgaaagcga aatcgacacg gcgtacggca
    19501 cccgcccatg aatatcaaac accggaacag caatacagga gacgccgagt tcgttttctt
    19561 ctctgtccat cgccgctccg ctttcacgaa tgtgcgccag ttcgtcgaac atcgcgggca
    19621 gctcggtaat ggtattgcgg gttaacggct ggatctcatg ctgatggttt tcccagtatg
    19681 acttcacgta gtccgggtga ccaaacgcca tgtagatctt gcccattgcg gaacagtaga
    19741 gcggcatatg ctggccaata taggcacggg ttcgcagcat cccggttgtg ggttccagct
    19801 tataaatcaa aatagcgtga tcgtcttcgc ggctggagaa gttaatggtt tcaccagtgg
    19861 cgatgttcag tgcctcaaga tgcggagcgg cgatatgaat gatattcagc gaagacagcg
    19921 ccttctgccc gacggcaata aatttggtag tcaggcggta actccctgcg gcgggcgcgg
    19981 tggtcacata gccgcaggac tgtaatccct gcaataagcg atggacggta ctcttattta
    20041 aaccagccag ctccgaaaga tgcgccaacg gacaaccgtt tggatagttg ctcaaaatct
    20101 caatcagcat caacccgcga aacagactct ggcttccggc tggacgctct ttttcctgcg
    20161 ccatctcgtt ctcttttttt cccatcactt ctttccccat tttgtcgcgt cctgatggta
    20221 gcgcaaagtg tgccgtagtt cacgatctcg acagataatt tataaccact tgatttttat
    20281 gtcttttgaa attcatcaat cagattgctt tgttaaaaag tgatcgatat atttgaaatc
    20341 aagtttcgca tattgaaatt ttaagccaaa aaagcgatca aaaaaacaag gaagcctggg
    20401 atgaaagtga catttgagca gttaaaagca gcctttaatc gggtcttaat ttcacgcggc
    20461 gttgacagcg aaacggctga cgcctgtgca gagatgttcg cccgcaccac cgaatccggc
    20521 gtttattctc acggcgttaa tcgtttccct cgtttcattc aacaactgga aaacggcgat
    20581 atcattcctg atgcccaacc caaacgtata accagcctcg gcgcaattga acagtgggat
    20641 gcccagcgtt cgatcggcaa cctgacggcg aaaaagatga tggatcgcgc cattgaactg
    20701 gctgccgatc acggtattgg tctggtggca cttcgtaatg ccaaccactg gatgcgcggc
    20761 ggcagctacg gctggcaggc ggcggaaaaa ggctatattg gcatttgctg gaccaactcc
    20821 atcgccgtaa tgccgccgtg gggcgcaaaa gagtgtcgca ttggcactaa cccgctgatc
    20881 gtcgccattc cttccacgcc gatcaccatg gtcgatatgt cgatgtcgat gttctcttac
    20941 ggcatgttag aagttaaccg cctggcgggc cgtcagctcc cggtcgatgg tggctttgat
    21001 gatgagggca atttgaccaa agaacctggc gttatcgaga agaatcgccg cattttgccg
    21061 atgggctact ggaaaggttc tggcatgtcg attgtgctgg atatgatcgc tactctcctt
    21121 tccgacggcg catcggttgc cgaagtcacc caggacaaca gcgacgaata cggcatttca
    21181 caaattttta ttgccattga agtggacaag cttatcgacg gtcccacccg cgatgccaag
    21241 ctgcaacgta tcatggatta cgttactacc gctgaacgcg ctgacgaaaa ccaggccatc
    21301 cgcttacccg gccacgaatt tactaccctg ctggccgaaa accgccgtaa cggtattacc
    21361 gttgatgaca gcgtgtgggc caaaatccag gcgttataag gagataagtc atgatttttg
    21421 gacatatcgc gcagcccaat ccgtgccgtt tgcccgccgc cattgaaaag gggctcgatt
    21481 ttctgcgcgc taccgatttc aacgccctgg agccgggcgt tgtcgaaatc gacggcaaaa
    21541 atatttatgc gcaaattatc gatttaacca cacgcgaagc ggtggaaaac cgtccggaag
    21601 tgcatcgtcg gtatatcgat attcagtttc tggcgtgggg tgaagagaaa atcggcattg
    21661 ctattgatac gggaaataat aaagtcagcg aatcactatt agagcagcgc gatattattt
    21721 tttatcatga cagtgaacat gaatcattta tagaaatgat accgggcagc tacgcaattt
    21781 tctttccgca ggatgttcac cgaccaggtt gtattttgca aacagcctct gagatacgaa
    21841 aaattgtggt taaagtcgca ttaacggcgc tgaattaatc gaataatcgt ctacgccaga
    21901 acgactgggt aatgttattg ctctaactat attaataatt taggttgctg cacatgcaac
    21961 ttgaattatg acagacatag cctcaaggaa tagctatgaa aaaaatactt gaagcaatac
    22021 tggcgattaa tctcgccgta ctttcctgta ttgtgtttat aaacattatt ttaagatatg
    22081 gttttcagac aagcatttta tctgtagatg aattgtcacg ttatttattt gtctggttaa
    22141 catttattgg cgcgattgtg gcttttatgg ataacgccca cgttcaggtc acctttctgg
    22201 tggagaaact ctcccccgca tggcaacgac gagtcgctct ggttacccat tccttaatct
    22261 tatttatttg tggcgcactg gcctggggcg caacgctaaa aacaattcag gactggagcg
    22321 attattcacc gatcctcggt ttacccatcg gcctgatgta tgccgcttgt ctgcccacca
    22381 gcctcgttat cgcattcttt gaactgcgtc atttatatca actcatcacg cgtagcaatt
    22441 ctctaacgtc gccaccgcaa ggagcttaat catggctgtg ctgatttttc tgggctgtct
    22501 gttgggtggt attgctatcg gtttgcctat tgcctgggca ctgttgttgt gcggggcggc
    22561 gttaatgttc tggctggaca tgtttgatgt ccagatcatg gcacaaacgc tggtgaacgg
    22621 agccgatagc ttctccctgc tggcgattcc gttctttgtg ctggcgggtg aaatcatgaa
    22681 tgcgggcggc ttgtcaaaac gcattgttga cctgccgatg aaactggtgg ggcataaacc
    22741 aggcgggctg ggctacgtcg gcgtgctggc agctatgatt atggcgagcc tttctggttc
    22801 tgccgttgcg gataccgccg ccgttgccgc tctactggtg ccgatgatgc gcagcgccaa
    22861 ctacccggtc aaccgggcgg cggggctgat tgcctctggc ggcattatcg cgccaattat
    22921 tcccccttcc attccgttta ttatcttcgg cgtttccagc ggattatcca tcagtaagct
    22981 gtttatggcg ggcattgccc ctggcatgat gatgggcgca acgctgatgc ttacctggtg
    23041 gtggcaggcc agtcgcctta atttaccccg ccagcaaaaa gcaacgatgc aggaaatctg
    23101 gcactctttt gtctccggta tctgggcgct gtttcttcca gtcatcatta ttggcgggtt
    23161 tcgctccggt ctgtttaccc ctaccgaagc cggggccgtt gctgctttct atgcgctgtt
    23221 tgtcgccaca gttatttacc gtgaaatgac cttcgccaca ctttggcatg tactcattgg
    23281 cgcggcgaaa accacctcag tggttatgtt tctggtggcc tcggcacaag tttccgcctg
    23341 gctgattacc attgctgaac tgccgatgat ggtttctgat ttactgcaac cgttggtcga
    23401 ttcaccgcgt ctgctgttta tcgtcattat ggtggctatc ctgattgtcg gcatggtcat
    23461 ggatttaacg ccgacggtat taattcttac cccggtatta atgcctttag ttaaagaggc
    23521 aggaatcgat ccgatttatt tcggtgtcat gtttatcatt aactgctcaa tcggcttaat
    23581 tacaccgcct atcggtaatg tacttaacgt tatttcaggg gtggcaaaac tcaaattcga
    23641 tgatgcagtc agaggcgttt tcccttacgt cctggtttta tattcattat tagtcgtatt
    23701 tgtttttatt cccgatctca tcatcctccc tttgaaatgg attaattaaa aggaaaatat
    23761 tatgaaatta cgctctgtaa cctacgcatt attcattgct ggcctggctg cattcagcac
    23821 atcttctctg gcggcgcaat ctttacgttt cggttatgaa acctcacaaa ccgactcgca
    23881 acatattgcg gcgaaaaaat tcaatgattt attgcaggag agaaccaaag gcgagctgaa
    23941 attaaaattg tttccggata gcaccctcgg taacgcgcag gcgatgatca gcggcgtacg
    24001 tggcggcacc atcgatatgg aaatgtccgg ctcgaataac tttgccgggt tatcaccagt
    24061 gatgaacttg cttgatgtcc ctttcctgtt ccgcgatacc gctcacgcgc ataaaacgct
    24121 cgacggcaaa gtcggtgatg atctgaaagc ctcacttgaa ggtaaaggac tgaaagtact
    24181 ggcctactgg gaaaacggct ggcgcgatgt caccaactcg cgcgcaccgg ttaaaacccc
    24241 cgccgacctg aaagggctga aaattcgcac caacaatagc ccgatgaata tcgccgcatt
    24301 caaagtcttt ggcgctaacc cgatcccgat gccatttgcc gaagtctata ccgggctgga
    24361 aacgcgcact atcgacgctc aggaacaccc gatcaacgtc gtctggtcag caaaattttt
    24421 cgaagtgcag aagtaccttt ctctgacgca ccacgcctat tccccgcttc tggtggtgat
    24481 caacaaagcg aagtttgatg gcttaagccc ggagttccag caagcgctaa tttcatctgc
    24541 gcaggaagcg ggtaactacc agcgcaaact ggttgccgaa gatcagcaaa aaatcatcga
    24601 cggcatgaaa gaagcgggcg tggaagtcat caccgatctc gaccgcaaag cctttagcga
    24661 cgcactgggt actcaggtcc gcgacatgtt tgtaaaagat gttccgcagg gtgctgatct
    24721 actgaaagcc gtggatgagg tgcaataaac catgacgcaa tactggctgg ggttagattg
    24781 tggcggtagc tggctgaaag ccgggctgta tgaccgcgaa ggccgggagg caggcgtgca
    24841 gcgcctgccg ctgtgcgcat taagcccgca gccaggttgg gcagagcgcg atatggcaga
    24901 actgtggcaa tgctgcatgg ctgtcattcg cgaactgctt acccattccg gcgttagcgg
    24961 ggaacaaatt gtcggtatcg gtatctccgc acaaggaaaa ggcttgtttt tgctggataa
    25021 aaacaacaag ccgctcggta acgccatttt gtcctcggat cgccgggcga tggaaatcgt
    25081 tcgtcgctgg caggaagatg gcatcccgga aaaactctac ccgttgaccc gacaaacctt
    25141 gtggaccggg catccggtgt cgctgttacg ctggctgaaa gagcacgaac cggaacgcta
    25201 cgcgcaaatt ggctgcgtga tgatgacgca cgactacctg cgctggtgtt taaccggcgt
    25261 caaaggctgt gaagagagca atatttccga gtccaacctc tacaacatga gtcttgggga
    25321 atatgacccg tgcctcaccg actggctggg gattgctgaa atcaatcacg ccctgccgcc
    25381 tgttgtcgga tctgccgaaa tctgcgggga gatcaccgct cagacagccg tactgaccgg
    25441 tctgaaagcg ggtacgcccg ttgtcggcgg cctgtttgat gtggtttcca ccgcactctg
    25501 cgccgggatc gaagacgaat ttacccttaa tgcggtgatg ggcacctggg cggtgaccag
    25561 cggcataacc cgcggtttac gtgacggtga agcgcatccg tatgtctatg gtcgctacgt
    25621 taacgatggt gaatttatcg ttcacgaagc cagccccacc tcttccggca acctcgaatg
    25681 gtttaccgca cagtggggag aaatctcgtt tgatgagatc aaccaggccg ttgccagctt
    25741 gccgaaggcc ggtggcgatc tctttttcct gccgttcctg tacggcagca acgccggact
    25801 cgagatgacc agcggtttct acgggatgca ggccattcac acccgcgcac acctgttgca
    25861 ggccatttat gaaggggtgg tgttcagcca tatgacccac ctcaaccgga tgcgcgaacg
    25921 ttttactgat gtgcacaccc tgcgcgtcac tggcggcccg gcgcactccg atgtctggat
    25981 gcaaatgctg gcggacgtca gcggtctgcg tatcgagctg ccgcaggtgg aagaaaccgg
    26041 ctgttttggc gcggcacttg ccgcccgtgt cggcaccgga gtttatcgcg atttcagcga
    26101 agctcaacgt gacttacagc acccggtacg caccctgctg ccggatatga ccgcccatca
    26161 gctttaccaa caaaaatacc aacgctatca gcatctcatt gccgcacttc agggctttca
    26221 cgcccgcatc aaggagcaca tattatgagc cgaccacttc tgcaactggc actcgaccac
    26281 tcatcacttg aagccgcaca gcgcgacgtg acgcagttaa aagacagcgt cgatattgtc
    26341 gaagcgggca ccattctctg tttaaacgaa gggcttggcg cggtgaaagc cttgcgcgaa
    26401 cagtgccctg acaaaatcat cgttgccgac tggaaggtcg ccgacgctgg tgaaacgctc
    26461 gcgcaacagg catttggcgc aggcgctaac tggatgacca tcatctgcgc cgcaccgctc
    26521 gcgacggtgg aaaaaggcca cgcgatggcg caacgctgcg gtggtgaaat tcagatcgag
    26581 ctgttcggta actggacgct ggacgacgcc cgcgactggc atcgtattgg cgtgcggcag
    26641 gcgatttatc atcacggtcg tgatgcacag gccagcgggc aacagtgggg cgaagccgat
    26701 ctggcacgca tgaaggcgct ttccgatatc ggccttgagc tttccattac cggcgggatt
    26761 acccctgctg acctgccgct gtttaaagat attcgcgtga aagcgtttat tgccgggcga
    26821 gcactggcag gctcagcaaa tccggcgcag gtggctggcg atttccatgc gcaaatcgac
    26881 gctatctggg gaggcaagcg tgcgtaatca tccgttaggg atttatgaaa aggcgctggc
    26941 gaaagatctc tcctggccgg agcggctggt gctggcaaaa agctgcggtt ttgattttgt
    27001 cgaaatgtcg gtggatgaaa ccgacgaacg gctttcacgt cttgactgga gcaccgcaca
    27061 aaggacttcg ctggttgccg cgatgatcga aacgggtgtt gggatcccgt cgatgtgcct
    27121 gtccgcacat cgtcgctttc cttttggtag ccgtgacgag gcagtgcgcg aacgggcgcg
    27181 ggaaatcatg agtaaagcga ttcgcctggc gcgcgatctc ggcattcgca ccatccagct
    27241 ggcaggttat gacgtctatt acgaagacca cgacgaaggc acccggcaac gttttgctga
    27301 agggctggcg tgggcagtcg aacaggcggc ggcatcacaa gtaatgctgg cggtggagat
    27361 tatggataca gcgtttatga actccatcag caaatggaaa aaatgggacg agatgctcgc
    27421 ctcgccgtgg ttcaccgtct acccggacgt cggcaacctc agcgcctggg gcaatgatgt
    27481 tcccgccgaa ctgaaactgg gcattgaccg tatcgccgcg atccacctga aagacaccca
    27541 gccagtcacc gggcaaagcc ccggacagtt ccgtgatgtg ccgtttggcg aaggctgcgt
    27601 cgatttcgtt ggcatcttca aaacgctgca tgaactgaac tatcgcggtt ctttcctgat
    27661 tgagatgtgg accgaaaaag ccaaagagcc ggtgctggag atcattcagg cgcggcgctg
    27721 gattgaagcg cgtatgcagg aggctggatt tatatgttag agcaactgaa agccgacgtg
    27781 ctggcggcga atctggcgct tcccgctcac catctggtga cgttcacctg gggcaatgtc
    27841 agcgcggtag acgacacgcg gcaatggatg gtaatcaaac cttccggcgt cgagtacgac
    27901 gtgatgaccg ccgacgatat ggtggtggtt gagatagcca gcggcaaggt ggtggaaggt
    27961 agcaaaaagc cctcgtccga cacgccaacg catctggcgc tttaccgtcg ctatgccgaa
    28021 attggtggca ttgtgcatac ccactcgcgc cacgccacca tctggtcaca ggccgggctg
    28081 gatctccccg cctggggcac cacccacgcc gattattttt acggtgctat cccctgcacg
    28141 cgacagatga ccgcagagga gattaacggc gaatatgaat atcagaccgg cgaagtgatc
    28201 attgaaacct tcgaagaacg tggcaggagt ccggcacaaa tcccggcggt gctggtgcat
    28261 tctcacggcc cgttcgcatg gggtaaaaac gccgccgatg ccgtgcataa cgccgtagta
    28321 ctcgaagaat gcgcctatat gggtctattc tcgcgccagc ttgcgccgca gctccctgcg
    28381 atgcaaaacg aactgctgga taagcactac ctgcgtaagc atggggccaa tgcctattac
    28441 gggcagtaat ccctcacgcc ggggcttcat cgccccggca ctacgaattg atatgttcct
    28501 tgctgtaacg ccgcttccac gctgctggcg ttaaaccagt atgtttctga aaaatctgcc
    28561 ggaaatagcc aacgtcatta aaccgtattg catggagcag gccgacaacg tcgagtcact
    28621 gcataacctg ttgctgcccg ccgatgcgcc atttacgaca tttgaaggca agggattgtt
    28681 cagccataag atcctgattc agacgccagg ttcccggtat gaacagagca atccagagca
    28741 acaacctttg tggcattacg acggagcacc agccgcaccg acatccaccg gtgaattaaa
    28801 aacgccagct caccccggtg acaaaggtaa aactctcatt acgatcaatc atcgggctgt
    28861 cggtaatctc ttccggcagc tcgctgtatc ctgcgctcgc catcaatacg acgtgctctc
    28921 ctatcgggta ttttgccgtc aggctgacat agggcaccca ggcatcctgc gcggaataac
    28981 ttgccagacc gctacgacgg gactcactct ctgaaatgcc atagtaatag tcactgaaat
    29041 tctcgtcata atagagaacg cccagcgcag gtgtcagcga caggcgacct atctgcattt
    29101 tgtggaatac cgatagctcc cccacccagc cgttgctgtt atccagaacg tccgcagccg
    29161 cagaggcttt cacggttccc caacgctcgt ggtgatacca ggcaaccccc gccatagccg
    29221 tactatcacg cttatcaagc tgttgcatgg cataatcgtc attatcggca gggtcaaatt
    29281 ccagcggcat ccaggatgcg gtcaggctaa gttcattttt ttcactttgc gacagaataa
    29341 aacctaacgt ggtctgacga acataaaaag aatcaccttc ataactaatc agcggaattg
    29401 cctttgtatt tttattataa ccgcgataag gcgattcatt ataagccgca ccagcaccaa
    29461 tggataattc agaagcagtt gcgcttgcca taaaaggcaa cgcaaataac gccacaatat
    29521 tgcggttaat taacatgtcc ggtattccat tttaaaat
//
LOCUS       DAPHDW010000048        28481 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.048, whole genome shotgun sequence.
ACCESSION   DAPHDW010000048 DAPHDW010000000
VERSION     DAPHDW010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.048"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1209
                     /locus_tag="RH057_002089"
     CDS             <1..1209
                     /locus_tag="RH057_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDV3128452.1"
                     /translation="TVNDDANDVAVTESYKPVWDWKISQFNVSDIKFETMINPQFADR
                     TYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFATGHLRIMAEGENFLSQLLNIEDE
                     VLNVLLRENFNEQLDTNVNTIISILNRRDIVLESLQPYLVINKDAVTPCTFLGDQTGD
                     RFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNCNGNYMQNFTRMKPLDEDTYAGI
                     KDYPVCFYDPKYKIMANHHGITFDEQRKRYIIGPITVSIDEMTNALDPVELAAIINKK
                     HHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLEALLACSQMLGINQVVAHNGNGG
                     RERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGAGPEITTPWKSYQHEKNRNGLMP
                     LIRRRTMLQL"
     gene            1575..2642
                     /locus_tag="RH057_002090"
     CDS             1575..2642
                     /locus_tag="RH057_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3128453.1"
                     /translation="MDKSKRHLAWWVVGLLAVAAIVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAHRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            2639..5374
                     /gene="rbbA"
                     /locus_tag="RH057_002091"
     CDS             2639..5374
                     /gene="rbbA"
                     /locus_tag="RH057_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HDV3128454.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVMVLGGDMRDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREVRINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPELLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYISLGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            5374..6498
                     /locus_tag="RH057_002092"
     CDS             5374..6498
                     /locus_tag="RH057_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3128455.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKVWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            6571..6846
                     /locus_tag="RH057_002093"
     CDS             6571..6846
                     /locus_tag="RH057_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HDV3128456.1"
                     /translation="MRTKVQALRKKQKNTLDQIVKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVRDWLLSIGVKP"
     gene            6843..7202
                     /locus_tag="RH057_002094"
     CDS             6843..7202
                     /locus_tag="RH057_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HDV3128457.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(7322..7723)
                     /gene="nikR"
                     /locus_tag="RH057_002095"
     CDS             complement(7322..7723)
                     /gene="nikR"
                     /locus_tag="RH057_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HDV3128458.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(7729..8535)
                     /gene="nikE"
                     /locus_tag="RH057_002096"
     CDS             complement(7729..8535)
                     /gene="nikE"
                     /locus_tag="RH057_002096"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HDV3128459.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWRGEPLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQRV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIVETQVVGDKLTFSSDAGRVLQNAVLPAFPVRRRTTEKV"
     gene            complement(8532..9296)
                     /gene="nikD"
                     /locus_tag="RH057_002097"
     CDS             complement(8532..9296)
                     /gene="nikD"
                     /locus_tag="RH057_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HDV3128460.1"
                     /translation="MPQQIELRNIALQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPCALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            complement(9296..10129)
                     /gene="nikC"
                     /locus_tag="RH057_002098"
     CDS             complement(9296..10129)
                     /gene="nikC"
                     /locus_tag="RH057_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HDV3128461.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWLPYDPQAIDLPSR
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGR
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            complement(10126..11070)
                     /gene="nikB"
                     /locus_tag="RH057_002099"
     CDS             complement(10126..11070)
                     /gene="nikB"
                     /locus_tag="RH057_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HDV3128462.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGGWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            complement(11070..12644)
                     /gene="nikA"
                     /locus_tag="RH057_002100"
     CDS             complement(11070..12644)
                     /gene="nikA"
                     /locus_tag="RH057_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HDV3128463.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALSKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            complement(12755..13342)
                     /gene="acpT"
                     /locus_tag="RH057_002101"
     CDS             complement(12755..13342)
                     /gene="acpT"
                     /locus_tag="RH057_002101"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HDV3128464.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRALLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRANWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            complement(13397..14446)
                     /locus_tag="RH057_002102"
     CDS             complement(13397..14446)
                     /locus_tag="RH057_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HDV3128465.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPIAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLELM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGIYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            14578..15795
                     /locus_tag="RH057_002103"
     CDS             14578..15795
                     /locus_tag="RH057_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3128466.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVTIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            complement(15799..16356)
                     /gene="dcrB"
                     /locus_tag="RH057_002104"
     CDS             complement(15799..16356)
                     /gene="dcrB"
                     /locus_tag="RH057_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HDV3128467.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(16429..17094)
                     /locus_tag="RH057_002105"
     CDS             complement(16429..17094)
                     /locus_tag="RH057_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HDV3128468.1"
                     /translation="MDVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            17315..17560
                     /gene="tusA"
                     /locus_tag="RH057_002106"
     CDS             17315..17560
                     /gene="tusA"
                     /locus_tag="RH057_002106"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HDV3128469.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            complement(17662..19860)
                     /gene="zntA"
                     /locus_tag="RH057_002107"
     CDS             complement(17662..19860)
                     /gene="zntA"
                     /locus_tag="RH057_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HDV3128470.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSTPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTLVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAALTIPVAESQRALVGSGIEA
                     QVNGEHVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAA
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(19934..20560)
                     /locus_tag="RH057_002108"
     CDS             complement(19934..20560)
                     /locus_tag="RH057_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HDV3128471.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            20701..21060
                     /locus_tag="RH057_002109"
     CDS             20701..21060
                     /locus_tag="RH057_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HDV3128472.1"
                     /translation="MSKPPLFFIVIIGLIIVAASFRFMQQRREKADNDMAPLQQKLVL
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            complement(21063..21332)
                     /locus_tag="RH057_002110"
     CDS             complement(21063..21332)
                     /locus_tag="RH057_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HDV3128473.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(21322..21918)
                     /gene="rsmD"
                     /locus_tag="RH057_002111"
     CDS             complement(21322..21918)
                     /gene="rsmD"
                     /locus_tag="RH057_002111"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HDV3128474.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRAVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQREAQGESDAD"
     gene            22068..23564
                     /gene="ftsY"
                     /locus_tag="RH057_002112"
     CDS             22068..23564
                     /gene="ftsY"
                     /locus_tag="RH057_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HDV3128475.1"
                     /translation="MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQTVVEEIVQAQEP
                     VKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVAESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSPEEWQAEAETVEIVEAAEEEAAKEEITDEELEAQAL
                     AAEAAEEAVIVVPPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISL
                     FRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEM
                     GEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTFR
                     AAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKS
                     HLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLD
                     GTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            23567..24235
                     /gene="ftsE"
                     /locus_tag="RH057_002113"
     CDS             23567..24235
                     /gene="ftsE"
                     /locus_tag="RH057_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HDV3128476.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            24228..25286
                     /gene="ftsX"
                     /locus_tag="RH057_002114"
     CDS             24228..25286
                     /gene="ftsX"
                     /locus_tag="RH057_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HDV3128477.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            25531..26385
                     /gene="rpoH"
                     /locus_tag="RH057_002115"
     CDS             25531..26385
                     /gene="rpoH"
                     /locus_tag="RH057_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HDV3128478.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMQGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            26656..27759
                     /gene="livJ"
                     /locus_tag="RH057_002116"
     CDS             26656..27759
                     /gene="livJ"
                     /locus_tag="RH057_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HDV3128479.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(27958..28341)
                     /gene="panM"
                     /locus_tag="RH057_002117"
     CDS             complement(27958..28341)
                     /gene="panM"
                     /locus_tag="RH057_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HDV3128480.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPAVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
BASE COUNT         6802 a         7374 c         7847 g         6458 t
ORIGIN      
        1 acagtaaatg atgatgctaa tgatgttgca gttactgagt cgtacaaacc cgtctgggac
       61 tggaaaatat cgcaattcaa tgtatctgat attaaattcg aaacaatgat aaacccccag
      121 ttcgctgaca gaacctattt ctccaattgt tcacctgttg atcccacaag taccagacca
      181 acctattttg gcgataccga tggctcagtc ggtgcagttt tgtacgcgct ttttgcaacg
      241 ggacatttga gaatcatggc tgaaggagaa aatttcctta gccagttgct taatatagaa
      301 gatgaagttc tcaacgtatt gctaagggag aactttaatg agcaacttga cactaatgtt
      361 aatacaataa tcagtattct gaacagaaga gatatagttt tagaaagtct tcagccttat
      421 ttagttatta ataaagacgc tgtgacgcca tgtacgtttc ttggcgacca aaccggcgat
      481 cgatttagta atatctgtgg cgatcaattt atcatcgatt tgttaaaacg cataatgagt
      541 atcaatgaaa atgttcatgt attagctgga aatcatgaaa cgaattgcaa tggtaactat
      601 atgcagaact tcacgcgtat gaaaccactt gatgaggata cctacgctgg gataaaagat
      661 tatcctgtat gtttctatga ccccaaatac aaaataatgg caaatcatca tgggataact
      721 tttgatgagc agcgaaaacg ttacattata ggaccgataa cagtctctat tgatgaaatg
      781 accaatgctc tcgatccggt cgaattagcg gcaattatca ataaaaagca tcatgctata
      841 attaatggca aaaagtttaa aactagccgg gctatctctt gtcgatcctt taatcgttat
      901 ttttctgttt cgacagatta cagacctaaa cttgaagcac tattagcatg ttcacaaatg
      961 ttaggtataa atcaagttgt tgcacataac ggaaatggtg gaagagaacg cataggcgaa
     1021 acggggacag ttttaggtct taacgcccgt gacagtaaac atgctggaag aatgttcagc
     1081 atgcataatt gccaaattaa ccctggtgca gggcctgaga taaccactcc ctggaagtct
     1141 taccagcatg aaaaaaacag aaatggactt atgcctttga ttagaaggcg cacaatgtta
     1201 cagctctgaa ccatagacaa catgaatatt aatgttacta tctggttgag actgttgata
     1261 aaacgtaaaa aggaatgctc tgttcctgca actgaacagg cgtgaaacgc tatccaacag
     1321 gatggatacc gttttgtcag ccagttttat agatcacact tatgagtatg cgtgtttcat
     1381 ttacggccag gaatgctgat gatgacaaca ttactacccg ttttcactaa gccttccccg
     1441 ttggcgctca atgctctgcg cgctggtcgt atttgccgtt tccttcttat cccggatgga
     1501 agaatccgct gaacgtcgtt cggcggcagt tgtaacacgc gtcgtttgct tacagggatt
     1561 atggcgggtc gattatggat aagagtaagc gccatctggc gtggtgggtt gtcgggttac
     1621 tggcggtggc ggctatcgtg gcgtggtggc tgttgcgccc ggcaggtgtg ccggaaggct
     1681 ttgctgtcag caatgggcgc attgaagcga cggaagtgga tatcgccagc aaaattgccg
     1741 ggcgtattga caccattctg gtgaaagaag gccagtttgt tcgagaaggt gaagtgctgg
     1801 cgaagatgga tactcgcgtg ttgcaggaac agcgactgga agccatcgcg caaatcaaag
     1861 aagcacaaag cgccgttgct gccgcgcagg ctttgctgga gcaacgccaa agtgaaactc
     1921 gcgccgctca gtcgctggtt aatcaacggc aagctgaact ggactccgta gccaaacgtc
     1981 atacccgttc ccgctcgctc gcccatcgtg gggctatttc tgcgcaacag ttggatgacg
     2041 accgcgccgc cgctgagagc gcccgagctg cgctggaatc ggcaaaagct caggtttcgg
     2101 cttctaaagc cgctatagaa gcggcacgca ccaatatcat tcaggcgcaa actcgcgtcg
     2161 aagccgcaca agccactgaa cggcgcattg ccgcagatat cgatgacagc gaactgaaag
     2221 ccccgcgtga cggacgcgtg cagtatcggg ttgccgagcc aggcgaagtg ctggcggcag
     2281 gcggtcgggt gctgaatatg gtcgatctca gcgacgtcta tatgactttc ttcctgccaa
     2341 ccgaacaggc gggcacgctg aaactgggcg gtgaagcccg gctgatcctc gatgccgcgc
     2401 cagatctgcg tattcctgca accatcagtt ttgtcgccag tgtcgcccag ttcacgccaa
     2461 aaaccgtcga aaccagcgat gaacggctga aactgatgtt ccgcgtcaaa gcgcgtatcc
     2521 caccggaatt actccagcag catctggaat atgtcaaaac cggtttaccg ggcgtagcgt
     2581 gggtgcgggt gaatgaagaa cttccgtggc ctgacgacct cgtggtgagg ttgccgcaat
     2641 gacgcatctg gaactggttc ccgtcccgcc tgtcgcgcaa ctggcgggcg tgagccagca
     2701 ttatggaaaa accgttgcgc tgaacaatat cacactcgat attccggccc gttgcatggt
     2761 cgggctgatt ggcccggacg gcgtcgggaa gtcgagcttg ttgtcgttga tttccggtgc
     2821 ccgcgtcatt gaacagggca atgtgatggt gctgggcggc gatatgcgcg acccgaagca
     2881 tcgccgcgac gtctgcccgc gcatcgcctg gatgccgcag gggctgggca aaaatctcta
     2941 ccacacctta tcggtgtatg aaaatgtcga ttttttcgct cgcttgttcg gtcacgataa
     3001 agcggagcgg gaagtccgaa ttaatgagct gctgaccagc accgggttag caccgtttcg
     3061 cgatcgtccg gcaggtaaac tctccggcgg gatgaagcaa aaactggggc tgtgctgcgc
     3121 gttaatccac gacccggaac tgttgatcct tgatgagcca acaacggggg ttgacccgct
     3181 ctcccgcgcc cagttctggg atctgatcga cagtattcgc cagcggcaga gcaatatgag
     3241 cgtgctggtc gccaccgcct atatggaaga ggccgaacgc ttcgactggc tggtagcgat
     3301 gaatgccgga gaagtgctgg caactggcag cgccgaagag ctgcggcagc aaacgcaaag
     3361 cgccacgctg gaagaggcgt ttataaatct gttaccgcaa gcgcaacgcc aggcgcatca
     3421 ggcggtagtg atcccaccgt atcaacctga aaatgcagag attgccatcg aagcgcgcga
     3481 tctgaccatg cgttttggtt ccttcgttgc cgttgatcac gtcaatttcc gcattccacg
     3541 cggggagatt tttggttttc ttggatcgaa cggctgcggt aaatccacca ccatgaaaat
     3601 gctcaccgga ctgctgcccg ccagcgaagg tgaggcgtgg ctgtttgggc aaccggttga
     3661 tccaaaagat atcgataccc gccgtcgggt gggctatatg tcgcaggcgt tttcgctcta
     3721 taacgaactc accgtgcggc aaaaccttga gttacatgcc cgtttgtttc acatcccgga
     3781 agcggaaatt cccgcgcgag tggctgaaat gagcgagcgt tttaagctca acgacgttga
     3841 agatgttctg ccggagttat tgccgctcgg cattcgccag cggctttcgc tggcggtggc
     3901 ggtgattcat cgcccggaga tgttaatcct cgatgaacct acttctggcg tcgatccggt
     3961 ggcgagggat atgttctggc agttgatggt cgatctctcg cgccaggaca aagtaaccat
     4021 ttttatctcc acccacttta tgaacgaagc ggaacgttgc gaccgcatct cactgatgca
     4081 cgccggaaaa gtgcttgcca gcggtacacc gcaggaactg gttgagaaac gcggagccgc
     4141 cagtctggaa gaggcattta tcgcctattt gcaggaagcg gcagggcaga gcaacgaagc
     4201 cgaagcgccg cccgtggtac acgacaccac ccacgcgccg cgtcagggat ttagcctgcg
     4261 ccgtctgttt agctacagcc gccgcgaagc gctggaactg cgacgcgatc cggtacgttc
     4321 gacgctggcg ctgatgggaa cggtgatcct gatgctgata atgggttacg gcatcagtat
     4381 ggatgtggaa aacctgcgct ttgcggtgct cgatcgcgac cagaccgtca gtagccaggc
     4441 gtggacgctc aatctctccg gttcccgtta ctttatcgaa cagccgccgc tcaccagtta
     4501 tgacgagctt gatcgtcgga tgcgtgcggg cgatatcacg gtggcgattg agatcccgcc
     4561 caatttcggg cgcgatatcg cgcgtggtac gcctgtggaa ctcggtgtct ggatcgacgg
     4621 agcgatgccg agccgtgccg aaacggtaaa aggttacgtg caggccatgc accagagctg
     4681 gttacaggat gtggcgagcc gacaatccac acccgccagc caaagcgggc tgatgaatat
     4741 tgagacgcgc tatcgctata acccggacgt aaaaagcctg ccagcgattg ttccggcggt
     4801 gatcccgctt ctgctgatga tgatcccgtc aatgctaagc gcccttagcg tggtgcggga
     4861 aaaagagctt gggtcgatta tcaaccttta cgtgaccccc accacgcgta gtgaattttt
     4921 gcttggtaaa cagttgccat acatctcgct ggggatgctg aactttttcc tgctctgcgg
     4981 cctgtcggtg tttgtgtttg gcgtaccgca taaaggcagt ttcctgacgc tcaccctggc
     5041 ggcgctgctg tatatcatca ttgccaccgg aatggggctg ctgatctcca cctttatgaa
     5101 aagccagatt gccgccattt tcggaacggc gattatcacg ttgatcccgg cgacacagtt
     5161 ttccgggatg atcgatccag tagcttcgct ggaagggcct ggacgttgga tcggcgaggt
     5221 ttacccgacc agtcattttc tgactatcgc ccgcgggacg ttctcgaaag cgctggatct
     5281 gactgatttg tggcaacttt ttatcccgtt gctgatagcc atcccgctgg tgatgggctt
     5341 aagcatcctg ctgctgaaaa aacaggaggg atgatgcgcc atttacgcaa tatttttaat
     5401 ctgggtatca aagagttgcg cagtctgctc ggtgataaag cgatgctgac gctgattgtc
     5461 ttctcgttta cggtgtcggt gtattcgtca gcgaccgtta cgccaggatc gttgaacctc
     5521 gcgccgatcg ccattgccga tatggatcaa tcgcagttat cgaaccggat cgttaacagc
     5581 ttctatcgcc cgtggttttt gccaccggag atgatcaccg ccgatgagat ggatgccgga
     5641 ctggacgccg ggcgttatac ctttgcgata aatattccgc ctaattttca gcgtgatgtc
     5701 ctcgccggac gccagccgga tattcaggtg aacgtcgatg ccacgcgcat gagccaggca
     5761 tttaccggca atgggtatat ccagaatatt atcaacggtg aagtgaacag ctttgtcgcg
     5821 cgctaccgtg ataacagcga accgttggta tcgctggaaa cccggatgcg ctttaacccg
     5881 aaccttgatc ccgcgtggtt tggcggggtg atggcgatca tcaacaacat taccatgctg
     5941 gcgattgtat tgaccggatc ggcgctgatc cgcgagcgtg aacacggcac ggtggaacac
     6001 ttgttggtga tgccgataac gccgtttgag atcatgatgg caaaggtctg gtcgatgggg
     6061 ctggtggtgc tggtggtgtc gggattatca ctggtgctga tggtgaaagg cgtgctgggt
     6121 gtaccgattg aaggctcgat cccgctgttt atgctgggcg tggcgctcag tctgtttgcc
     6181 accacgtcaa tcggcatttt tatggggacg atagcgcgtt caatgccgca actggggctg
     6241 ctggtgattc tggtgctgct accgttgcaa atgctttccg gtggatccac gccgcgcgaa
     6301 agtatgccgc agatggtgca ggacattatg ctgaccatgc cgacgacaca ctttgttagc
     6361 ctcgcgcagg ccatcctcta ccggggtgcc ggattcgaaa tcgtctggcc gcagttcctg
     6421 acgctgatgg caattggcgg cgcgtttttc accattgcac tgctgcgatt caggaagacg
     6481 attgggacaa tggcgtaagg aagggaaact tgacttcata ctgtgatgtc atactattga
     6541 tgtcataaag tgaagtcata aggacatgcc atgagaacca aggtacaggc tttacggaag
     6601 aaacaaaaaa atacattgga tcaaatagtt aaaactcctg ttcctcaagg gatcaagtgg
     6661 tctgatatag agtcactggt taaagcatta ggcggagaaa ttaaggaagg aagaggttcg
     6721 cgttgcaagt tcatactaaa tatgagcgtt gcgtgtttcc atcggcctca tccgtcgcca
     6781 gataccgata aaggcgctgt agaaagcgtg cgtgactggt tactaagtat aggggtaaaa
     6841 ccatgatcaa actgaagacg ccaaactcta tggagattgc cgggcaacct gcagttatca
     6901 cttacgtgcc agaactgaat gcatttcgtg gcaaattcct gggcttgtca ggttattgtg
     6961 attttgtctc tgacagcatt caggggctgc aaaaagaagg ggaactttcc ctgcgagagt
     7021 atcttgagga ttgtaaagct gcgggtattg agccgtatgc gcgtacagaa aagattaaga
     7081 cctttacgtt acgctatcca gaatcgctga gtgaacgcct taataatgcg gctgcgcagc
     7141 aacaagtctc tgttaatacc tatattattg aaacactgaa tgagcgtttg aatcatttat
     7201 aaattttagc ggcgtacatt acgcagaata tctgccttta caggatctat ctatgcaact
     7261 gaacttaatt ttataaatta agttcagtta atatcttttc tttcctcgtt tcagaaataa
     7321 ctcaatcttc cttcggcagg cactgcaaat gcccgtgccg cacgccgcgc tgggcgataa
     7381 cgtcatcggc aaaatgctgc acgtcgccca tatcaccttt cagcacggcg atttccagac
     7441 agtcgtcgtg attgatatgc acatgcagcg tggcgacgga gagatcgtgg tgatggtgct
     7501 gggtggagac aatgcggctg gctaagtcgc gtttttcgtg ttcatacaca taggagagca
     7561 ccgcgaaacc ttgcgtgccg tgctgctgag tggcctcctg ggccagagcg ctacgcagaa
     7621 tatcgcggat agcttcggaa cgattgttat aaccacgacg ctggctcagg ctgtccagcg
     7681 tctccagtaa atcgtcatca agcgtgatgg tgactcgttg catttgcgtt aaaccttttc
     7741 tgtggtgcga cggcgcacgg ggaatgcggg taataccgcg ttttgtagca cacgtccggc
     7801 gtcagaggaa aaggttaatt tatctcccac cacctgggtt tcgacgattt gtccgttgtc
     7861 catcaccatc acccgctgac agaagcgttc caccaggcgt aagtcgtggg tgatgaacag
     7921 gcaggcggtg ccaaactgtt gttgtagctt tttcagcagg cgaatgacgc ccgcctgtaa
     7981 cacgagatca aggttagaaa ctgcttcatc cagaatcagc agcttcggtt cgaccgccag
     8041 cgcgcgagcc aggcagacgc gctggagctg gccgccgctt aactgcggtg ggcgcttgtc
     8101 gagcacgctg tcgtcgagat cgaccgcctt cagcatttcg ctggcgcgcg ccagttgttc
     8161 ggattttttc agtgatagca ggtggcgcat cggttcacgc aggatctcgc gcacggtttt
     8221 gcgcggattc acggcgctga tggagtcctg aaataccatc tgaatatcgc ggcggaacgc
     8281 tttacgctgg gcgcgattga gtttcgccag cggttcgccg cgccagctaa tattcccttg
     8341 tgagggagac tctaagccta ccagcagccg cgcgagggtg ctttttccgc agccgctgcg
     8401 tcccagcagg gcgacggttt cgccgctttt cagggtcagg gaaacgttat tcagcaccgc
     8461 ctgatgttga tgttttccgc taaatccacc gtgggcatag tgatgggaaa ggccgcagac
     8521 gttaagtaag gtcatgatgc cagctccata ccgtagaggg cgagatgagc ggaaaccagg
     8581 ctgcgcgtca ccgtatgttt gggggcgtta aacagcgttt ctacatcgcc ctgttcgaca
     8641 attttacctt gtgacatcac cgccacgtca tccgccagac gcgccacaac gcccatatca
     8701 tgggtgacca gcagcattcc cggcgcttgt ttttgcataa tgctttccag cagatcgagg
     8761 atgcgcgcct gtgctaccac gtcgaggtcg gtggtcggtt catcggcgat gataaacggt
     8821 gattcacaca gcaccgccat cgcaatcatc atgcgctgca acatgccgcc gctcatctcg
     8881 aacgggtaca gcttcagcac gcgcgcggcg ttttccagcc ccaccgcttc tatggcagcg
     8941 gtaagcgtag cgtcatcggc gggtttccct aacgccaggc aggtttcccg cgcgtgggtg
     9001 tgcatggtgt gcagtggatt aaaggcgctg cgcgggttct gcatgatggt ggcaattttg
     9061 atgccgcgca gggcgcaagg cgaaaccggt ttgccatcgg ctaaaatttc ccccgccgtc
     9121 tggcgaacgc cagcgggcaa aatgcccagc gtcgcggcgc aggttaatga tttcccgctg
     9181 ccgctaccgc cgactaacgc cagcacgcgc ccgcgttgca gggttaacga tacaccgtgt
     9241 accagcggct gcgcggcctg tagcgcgata ttacgtagtt caatctgttg cggcattagt
     9301 gtgcgtgctc cgtcaccaga tgaggatcca gatgatcgcg cagtgcgtca cccaccaggt
     9361 taaaggccat cacgctgata aacagcgcca gccccggcca gaacatttgc agcggctggg
     9421 tccagatata ctggcgcgcg tcgttaatca tcacgcccca ttcggcggtc ggcgcggtca
     9481 caccgaggcc aaggaaagac atccccgcga cgtgcagcat catatggccg atatccagcg
     9541 ttgccagcac cagcagcgaa gggatcaccg cgcctgccag atgatcgaca aacacccgca
     9601 catggcccgc accggaaagc cgtgacgcca gcacaaactc gcgttggcgt agtgaaatca
     9661 ccaggctgcg caccatgcgt gcataccacg cccagtgcga cagggcgatg gcgataatta
     9721 cgttggtcag cccggtgccg agcacgccaa ccataaagaa cgacagaatc gaggtcggga
     9781 aggtcataaa catatcggcg acgcgcatgg tggcctgatc aacgcgtccg ccaatcagtc
     9841 cggcgctgcc gccaatcacc agccctaatg tcagcaccag cagcaggcag gccattaccg
     9901 aaccgagcga cacgcgggtc gccgccatca gccgcgagaa aatatcacgc cctaagtgat
     9961 cggtgcccag ccagtgctgc gcatccggcg aaagcaggcg cgacggcaga tcaatagcct
    10021 gagggtcata cggcagccac cactggctgg taagcgcaat cagcgccagc agggcgataa
    10081 tgatcagcgc caggcgtacc gaccagcggg aagagaggaa aaagttcacg cgtgcgctcc
    10141 ttcatgacga cgaatgcgcg ggtccagcgc ggcgttgagc aaatcgacaa tcaaattaca
    10201 gaccacaaaa accaccacca tcatcagcgt aaagcactgg atcaccggat agtcacggtt
    10261 aaaaatcgcc gacaccgcat agcgcccgac gcccggccag gcaaagatgt tttcgataat
    10321 catcgtcccg ccgatcagtt cgccgatgtg catccccacg gcggtgatca tcggcagcga
    10381 ggcattgcgc aggatgtggc gacgttcggt ctgtttgtcg ttcaggccgc gcagacgcgc
    10441 ccaggtgacg tgacgctgac cggcgacgtc cagcatactg gcgcgcagta aacgcgcgtt
    10501 aatcgccagc gacataaagg caatggaaac cgcaggcaaa atgatgtgct gccagccgcc
    10561 gtaacccatc gcgggtagcc attgcagata caccgaaaac gccatcacca gtaaaaacgc
    10621 cagccagaag ttaggcatcg acacgccaag aaacgcgata aagcgcacgg cgaaatccgg
    10681 cagacggtcg cggtggcgcg ccgcccagat accgagcggt acggaagtga gcagaattaa
    10741 taccagcgcc gcacctgcaa gttccagcgt ggcgggcagg aagttcagca tatcgtccag
    10801 taccgggcgt tggctggcga atgagatacc aaagtcaaga tgcagcgcct tccacaacca
    10861 ggtgccgtac tggacgtaca gcggctgatc cagtcccagc atggtgcggg tagaggccag
    10921 catctccggc gtcggcggca ggttagacag acgcaaataa tcgagcgccg ggtcgccggt
    10981 accgaggcgc agcatcagaa aaatgatcac cgaggcggcg agcaccatcg ggatcagcag
    11041 cagaaagcgg cgtaatacgt aacgcaacat taaggtttca ccggtttaat ctgttcgaac
    11101 ggaatttcgg tggcgatcgg cgcgtagggg atattaccca gctccggttt tgataccacc
    11161 atcatcgaga tgtagctgat aggcagataa accgcctcgt catgcagacg ggtcagaatg
    11221 tcgcgataca gcgcctgacg ttgcgtttcg tcatgggtcg ccagcacttc gccgatctct
    11281 ttatcaatca gcggcttatc ggctaatcct tgctgtgcct ggaagtcagc gtgcgacggt
    11341 acgcgcattg aactcaggaa ggcgtgtgga tcgtatggcg cgccccaggt gcggtggaaa
    11401 atcatgccaa aacgaccgtc gcgctgacga gcatagatac tgctctcttc ttcgccaatc
    11461 agcgagacat ctgcgccaat ctggcgcata tcagcctgaa tgatttccgc catcgatttg
    11521 cttaacgcat cggtgccgat gaacgacagt tcaatgcgca gcggctgacc atttttctcg
    11581 cggatgtctt tgcccgcagg cagcgtccaa ccggcttttt ccagcaactc tttcgctttc
    11641 tgcggatcgt actggcgcgg tttcaggccg agattagcgt aaggcacaga aggggcaaac
    11701 agggtgtcgg cgacctgctg ggtgccatat aacgcgttat caatcagcga ttttttgttt
    11761 accgcgtaat taagagcttc acgtactgcc agctcgttgg tgggggcttt ggcggtattg
    11821 agcgccagca tcacggtttc gatcggctgt gacagttggg tgtggtaagc cggattctgg
    11881 ctaaagcggg cgaaggtatc gagcggtaat aacccttcgt ttccgtacag caggtcgata
    11941 tcgccagttt caaacgccac cgcgcgggta gtcgggtccg ggatgacgtt aaaggtgatc
    12001 tttttaatcg ctggcttttc gccccagtag ttttcgttac ggacgaagac atcgtactga
    12061 ttcagtttcg attcctgcaa aatccatggt ccggtgccaa tcggcgcttt aattccgttc
    12121 atggtttcat ggtttttaaa ctgcgaggga gcgataaaac ggaaaggacg gggcagggcc
    12181 agttcttgca ggaaaggata gtaggcgctt ttcagggtaa tttgcagctc tgttttactg
    12241 agtgctttaa catcaacaat ctggtttgcc agctccagcc aggcgtgacg ttgacggtta
    12301 tcgagcactg cgcggaagtt ttctgccgcc gcctcggcat cgaacggttc accgttggag
    12361 aatttcacgt catcacgcag ggtgaaggtc caggttttac catcttctga atgagtccag
    12421 ctttttgcca gccacgggat caccgaaccg tctgcctgat atttcaccaa tggttcataa
    12481 accatgctct gggcgaacat ctggttaggc gtgtaaaggt gcgggtttag tggcccgaca
    12541 ttcaccggcc aggcggtggt gatttcatct ggtgcagcgg catggacgat aaaagacgca
    12601 caagccagca gcgcaaatag agtgcggcgg agtgtggaga gcatggtaac cccaatggat
    12661 taaaatagat ggcggaaata agtatgacga ttttaagtat tcgtcatact gattacctgt
    12721 tctggatcaa tagatgggca gtcgggtggg cggatcagtt aactgaatcg atccattgca
    12781 cactgtcggc ggtgagcgta aagggagtag gggtgcagat cgccaggctt aaattttcaa
    12841 gctgacaatg gctgactgac agcgaggagt gataggtgct gtctacgctg acgatttgcc
    12901 aggcgctgcc gccacgctgt ttaacgatgg cttctttgcg cgtccagatg cgccagaaca
    12961 tttccagttg ctgatcagga tgcacggcgt ccatctcagc gtgttccccg aggctgaata
    13021 cggcgttcgc cagccagcgc cagttggcgc gcgggcgaat aacttcgata tcgcagccga
    13081 cttcgccttc atcactcaac agcagggcga tatcgtcacc gctatggctt aagttgaacc
    13141 atagcggcgt ttccggcgca aatgcaggtt tgccttgttc gccatagatg atctccggta
    13201 gcggggaaag cgtgtgcgaa agcaatgcac gccccgccag ccagcgttcg cgtcgtggac
    13261 cttgcggtgc ttgctcgcgt aaacccggtg gcagtggagc tgcgcttaag gtcgaaactt
    13321 tccccagaac tatccgatac atatcagggc caacgtttaa tggaaaatga aagtgcgtat
    13381 cgtatcactt gtcgcctcat cccggtaacc gacttttcgg tctgcctggc cccagtaaaa
    13441 tcgccagttt gctaccgcct ttggtggttt ccatccagat tttacacaca ctggttaatg
    13501 gcaccgaaag tagcatccct accgggccga gcagccatcc ccaaattaac aatgaaagaa
    13561 ataccaccat ggtggacatc cccaggcgat ggcccatcat ccgtggctct aaaatattgc
    13621 cgatgaccat atggacaact aaaaacaatg cgccgaccag aatacattcg taaataccat
    13681 taaacagcag cacctgaatc attggcggta cggcggaaat taccgcgccg atattgggca
    13741 cgtagttaag caaaaacgcc agtactgccc acatcagcgc aaactgtacc cccatcagct
    13801 ccagccccag ccagacgatg acacctgtcc ataaactgag tagcgtcttc aatgcaagat
    13861 agtgcgaaac gcctttaagt gcgcggtgta atcccgcgat gtgaatctgt ggattattca
    13921 gcgcaaaacg cattttgtaa gggacgtggc gcacttcaaa cagcataaaa actacggtca
    13981 tcaccagcaa aagcacgctc gccattgccc cggaaagccc ggtcattagc gctgtggtga
    14041 aggtaaccac tttttccgag tccatccgct gcagcattcg ctccggcgac atatgcaaat
    14101 taagaaaagg caacatctcc tgcaatttaa aaagtttgcg cgtcagctcc ttattaaact
    14161 tcggcagcat agagataaat tcgttaaacg atgccgccag tacgccgacc agcgcagtta
    14221 gtgcgatcag catcaccacc accacaatcg taatggcaat ggggcgttgt actccccgac
    14281 gaataaacca ggtgacgagc gggttgagga cgatggcaaa aaacagcgcc agtaacagct
    14341 gcacaatgat atctgccgct gcgtgaatgc ccgcgaggat cactaccagc gaggccagct
    14401 tgagcagaat gtgcatgccc gttttatcgg gttgaggggt ttccattggg gcttccttgt
    14461 gactttttgt attaagtgta gcgggagtca cgccagcaat attctgattc ttactgctga
    14521 ttttcacgtc agcacgtggt aaaaatgaaa cactgttgta aaaatgtggt gatcctcatg
    14581 cccgaacccg tagccgaacc cgcgctaaac ggattgcgtc tgaatttgcg cattgtctcc
    14641 attgtcatgt ttaacttcgc cagctacctc accatcgggt tgccgctcgc tgtattaccg
    14701 ggctatgtcc atgatgtgat gggctttagc gctttctggg caggattggt tatcagcctg
    14761 caatatttcg ccaccttgct gagccgtcct catgccggac gttacgccga tttgctgggc
    14821 cccaaaaaga ttgtcgtctt cggtttatgc ggctgctttt tgagcggtct gggatatctg
    14881 acggcaggat taaccgccag tctgcccgtc atcagcctgt tattactttg cctgggacgc
    14941 gtgatccttg ggattgggca aagttttgcc ggaacgggat cgaccctgtg gggcgttggc
    15001 gtggttggct cgctgcatat cgggcgagtg atttcgtgga acggcattgt cacttacggg
    15061 gcgatggcga tgggtgcgcc gttaggcgtc gtgttttatc actggggcgg cttgcaggcg
    15121 ttagcgttaa tcattatggg cgtggcgctg gtggccattt tgttggcgat cccgcgtccg
    15181 acggtaaaag ccagtaaagg caaaccgctg ccgtttcgcg cggtgcttgg gcgcgtctgg
    15241 ctgtacggta tggcactggc actggcttcc gccggatttg gcgtcatcgc cacctttatc
    15301 acgctgtttt atgacgctaa aggttgggac ggtgcggctt tcgcgctgac gctgtttagc
    15361 tgtgcgtttg tcggtacgcg tttgttattc cctaacggca ttaaccgtat cggcggctta
    15421 aacgtagcga tgatttgctt tagcgttgag ataatcggcc tgctactggt tggcgtggcg
    15481 actatgccgt ggatggcgaa aatcggcgtc ttactggcgg gggcagggtt ttcgctggtg
    15541 ttcccggcat tgggtgtagt ggcggtaaaa gcggttccgc agcaaaatca gggggcggcg
    15601 ctggcaactt acaccgtatt tatggattta tcgcttggcg tgactggacc actggctggg
    15661 ctggtgatga gctgggcggg cgtaccggtg atttatctgg cggcggcggg actggtcaca
    15721 atcgcgttat tactgacgtg gcgattaaaa aaacggcctc cggaacacgt ccctgaggcc
    15781 gcctcatcat cttaaaactt actgaataac cagcgtatta atgatgtttt ctgcggtggt
    15841 ctgcgctttt tgctgatcgt cagcgggcag cgtaatttgc atggtcagca gttgattacc
    15901 cacgttaccc agaataacgg aagagtacgc cgtctggcct ttcgcggaga taatactgtc
    15961 taactgctgc attttgtgac ctttcagctc aatggcttta ttggttacca cttgcagctg
    16021 cggatcgcgg ctacgttgct gatcttccag acgcttcgcc agcaccgcca gatcttcttt
    16081 cggatcatcg cccatgatga cgatgactgc tttctgcccg gtggcgtcgg accagacatg
    16141 catgttattg gcctgcgttc ccagcttacc gctctggtcg gtcatatccg ctggcagcga
    16201 gaaacttaac ttgccatcaa gcaggttgac gggattcccg gtagcattac tttccgcgac
    16261 cgaaccctgc gccgtagcgt tagtgtcttt atcatcacag gccgcaagcc ccataaccag
    16321 caggccaatt ccgacatatt taaccagatt gcgcattgac ttcttccttt cgataaacgg
    16381 ccataacggc tcattcatcc atcttatcac aactctgata acgaaccttt aactcgcctg
    16441 caaagcgttg atttcggatt tatctgccag tcttttcaac agcatattga gtaatacgcc
    16501 atacattggc aggaagaaaa cgatactgat taacactttg aaacagtaat cgaccagcgc
    16561 gatttccatc cagtgttcag ccataaaggc atccgggcta cgccagaagg caatgaagaa
    16621 aaaggccagc gtgtcgctga cgttaccgaa cagtgtggac gctgtcggtg ccagccacca
    16681 gcggcgactt tgacgcaggc ggttaaaaac gtgcacgtcg aggatttgcc ccagcgcgta
    16741 ggccatgaaa ctggcggtgg cgatacgggc gacaaacagg ttgaagtggg cgagtgcgcc
    16801 gaatccctgc catgaaccca tatagaacag cgacgagatg acataggaga ttaataacgc
    16861 agggatcatt accgcgaaga taatgcgtcg ggccagcggt gcgccaaaaa tacgcacggt
    16921 caggtcggtc gcgagaaaga taaacggaaa actaaacgcg ccccaggtgg tatgaaaacc
    16981 aaaaatggag accggaagct ggaccagata gttactggag gtgatcacca gcagatgaaa
    17041 taacgataac cagaacaacg ccttatagcg ttgagtttgc gagaaaacgt ccatattgta
    17101 cctttttgat taaccattgg ggtgagggaa cccaatacgt acgacacgtc tgttatctta
    17161 acataataac gtgcttaccc ttttttcgag ccgccgcatg atactgcttt acgttgacaa
    17221 tgcaatggtt aattttcacg caatcgttaa cctggtttgc ttacggactc acagggcgta
    17281 aactagcgcc gtttttttaa gtgatgagaa gaaaatgacc gatctctttt ccagccctga
    17341 ccacacactc gacgcacttg gcctgcgctg cccggaaccg gtgatgatgg tgcgcaaaac
    17401 tgtgcgcaat atgcagcctg gcgaaacgtt gctgattatc gccgacgatc cggccactac
    17461 ccgcgatatt cctgggtttt gtacctttat ggaacacgaa ctggttgcga aagagacgga
    17521 tggactgcct tatcgttatt tgattcgtaa aggcggttga tggggaaaga ttggcttcga
    17581 tgccgccttt tcccctcacc ctaaccctct ccccagaggg gcgaggggac cgatcgcgct
    17641 caatgttgcg atcggtttgc cttatctcct gcgcaacaat cttaacgcat tcgctgtcac
    17701 cagcaccgtc gcacccgtat ctgccagcac cgccagccat agtccggtca tccctaaaag
    17761 cgtggtgacg aggaagatcc ctttcagtcc cagcgcaatg gtgatgttct ggcggatatt
    17821 ggcgtgagtg gcgcgtgcca gttcaatcat ttgcaccagg ccgcgcaggt ggttatgggt
    17881 taatgctgca tcagcggttt ccagcgccac gtctgtgcca ctgcccattg caatcccaat
    17941 ggcggcagct ttcatcgctg gcgcgtcgtt aataccatca ccgaccatcg ccaggggcgc
    18001 atgttgattc agcttggtca ccgctttgac tttatcttcc ggcaataggc ccgctttaaa
    18061 ctccagcccc agctccccgg cgattgccgc cgctgcacgt ggattatcgc cggtgaggat
    18121 cactcctttg acccccagcg cgttcagttc actgatggca gttgcagcat cggcgcgcag
    18181 ggtatcctgc aatgcaatga tacccagcac gtcatcgtta cgtactacca gcactaccgt
    18241 ttgcccggcg ctttccagtt cattaatcaa accagcaaat gcatcagcgg gatgtttccc
    18301 tgcagcgcat atcaacacgt gctcaccgtt aacctgcgct tcaatgccag acccgaccag
    18361 cgcccgctgt gactcggcga cgggaatggt cagtgcagca acctgtgctt ctcgcacgat
    18421 ggcctgcgcc agtggatgcg tcgcgccttg ctcgaccgcc gccgccagtg tcagcagttc
    18481 agattcacta atacccgttg ccggatgaat cgcggtaacg cgcggtttac cgacggtcag
    18541 cgtaccggtt ttatcaaacg ccaccagggt aacacgaccc agttgttcca gcgccgctcc
    18601 gcctttaatt aacgccccac gacgcgctgc tgctgccagc ccggaggtaa tcgccgcggg
    18661 cgtggagata actaacgcgc acgggcagcc aatcagcagc agcgtcaggc ctttataaat
    18721 ccactcctgc cagctggcgg caaacagcag cggcggcacc agcgttacca gcagggcgac
    18781 ggccataatc gctggcgtat aaatacggct gaaacggtcg ataaaccgct caatgggggc
    18841 gcggcgctct tcggcttctt cgatcagttt cagaatccgg tcaatggcgc tggcacccgg
    18901 ttctgacagc acttccagcg ttaccagacg gtctacgctg gtggctccag cagggacttt
    18961 atcgcccgtc gcgcgctcca ccggaatgga ttctccggtc agggcgcttt catcaaaact
    19021 ggcaaacggt gagagcaatt taccgtcggc aggcaaacgc ccacctgcgg cgacttcaat
    19081 cacatcgcca gggcgcaggc tgttaatcgc cacctcttcc cgctcaccgt tacgcaggcg
    19141 cgtggcggtt tctggtttca gcgccattaa cgcgctgacc ccttgacgtg cgcggctggc
    19201 ggcccagcct tccagtcgtt cgccaatcaa aaacagcagc aacaccatcg cagcttcagc
    19261 cgttgcgcca ataaacagcg caccaatagc ggctacgctc attaacgttt caatggcaaa
    19321 gtagctgccg gatttgatca accgtaatgc ctgacgagca atcgggtaca gcccaaccag
    19381 cgtggtcgcg ataaacgcca gttgcccgaa cggatgattg aactgctcca aaccccagct
    19441 gattgccatc atcacgataa gcgtaatcag cggcagattc tctttcaggc gcgatgcttg
    19501 tggctcgtcg gcggcctgct catcgcgcag ggaatagccc gctttttgca ccgcagattc
    19561 aacctgggca cggatgtcat tgtcggcatc aaccaccagt ttttcggtgg cgaacaacac
    19621 ctgtacctga ttcacgcctg caagctggcg cacggcattt tctaccttgc gcgcacaggc
    19681 ggcgcagtcc atgccgctga ctttccagct atagcgggtg ccggagacgt tttcagagag
    19741 agttggcgtg ctggaacatg cgccgtcgca gcaacagtcg ttggcgttct gtaccgtggt
    19801 tagcggtttg aacgcagcaa attgaggggc tttcttgccg tgattgtcag gagtcgacat
    19861 ggcatcctcc ggttaagttt tttctcatta accgaaggat acactctgga gtcgactcca
    19921 gagtcaagtt ttatcagaga tacagcgagc ggacgatcag gaagtgaccg gcaaagtagc
    19981 aggccgcagc aatcgcgtta tccgcacgga agcgtcggcg atagtgacta cccagccaga
    20041 caaagttact gataaacagc aacgaagcgc cgacaaacgc agagagcgcc ggagcggtcg
    20101 gacggaagaa ccacagttca cctgccagcc acaccatcac cagcgtcatg ccgataaacg
    20161 tgcagatagg ccaacgatac tcttccagcc gcgtccagat aatcgccagt aacagcgcgc
    20221 ccagaaccag cagcaccagt ggcagaggcc agaagaaaga gagcgtcatc tgactggcga
    20281 aatagatggt gtacagcagg tgcgagagga aaaacgcgcc gatggcgtac atcagacgtt
    20341 gacgtggcaa cagggttagc gcatcgccca gcagtgaggc gcacagcccc gccagcacca
    20401 gatagctaat ggcgtcgaac atcggcgctt gccaggccag taacagcagg agaagaaggg
    20461 ttaatggttt aaatacccag cgttgccagg ttggcccacg atacgatgca tccacagata
    20521 gccatgcgga aagacagaca gcgataaacg accaaagcat cttagttcct tgaattcgtt
    20581 attttttcgt aagcggaatg cctacggtct tgcttccagt ttagtggcga gggtggacgg
    20641 atgacaacac cataactggc agggtatgct taattccgca ttttccgatg agggataaag
    20701 atgagcaaac cacctctttt ctttattgtt atcattggct taatcatcgt cgccgcatcg
    20761 tttcgtttta tgcaacaacg acgggaaaaa gctgataatg atatggcacc actccagcaa
    20821 aagctggtgt tggtgagcaa caagcgggaa aaaccgatta acgatcgccg ttcgcgccag
    20881 caggaagtga ctccggcagg caccagtatg cgctatgagg caagcttcaa accgcaaagc
    20941 ggaggaatgg agcagacgtt tcgcctcgac gcccagcagt accacgccct gacagtgggc
    21001 gataaaggta cgctgagcta taaaggaacg cgctttgtca gctttgtagg cgaacaataa
    21061 ggttattttt taactttaaa tttcttctgc cagaccagca gttcaaacac gccgaaaagg
    21121 aaaatccgta ccttttcggc ggtggtcatc tgcgggccat ctttcggtaa agtggatttc
    21181 aatagcgcca gctgcatacc atgcatcagc acggtaaaaa tcagcgccac gttaacgaaa
    21241 atattcagcg gacgtgggaa gggatgcacc aggttgagga ttaaaaatcc ccaaacacag
    21301 agcattaaca aacgaccaat attaatcagc atcactttct ccttgtgctt cgcgttgata
    21361 cagccgataa gccacctgac ccgccacttt ttcccgatgt aatgaccagt ttgccggaac
    21421 agtgggcaga ccgttttcga cttcgctttc gacataaatc agggcttcgt cagccagcca
    21481 gccgttatct tccagtaaat ttatcgtctc ttctaacaag ccacggcgga acggtggatc
    21541 gacaaacacg atattatgcg gtgtgccttt ttgcgccagg aatgacattg cgttgctgtt
    21601 caccacgcgt gcattgcctg cttttagtgt cgccagattc ttaattaact gctgagaaac
    21661 cgcgcgatcc atctcaatca acgtggcccc cgcagcatag cgcgacaacg cttccagccc
    21721 cagcgcgccg ctcccggcga agcaatccag acattgggcg tcaacaatga ccggagccag
    21781 ccagttaaac aacgtttcgc gtacgcggtc ggtggtgggg cgcagacctg ggctatcagg
    21841 aaccgggagt ttacggcctc gccactgccc gccaataatg cggatttggc cgctgccgga
    21901 atgattcggt tttttcatga taaattgctc aatccgccag ataacacata cttgcaggcg
    21961 gtgatgtgaa ttaagttaag tgatagacta tttcatcatt tttttagctg ctatgtacat
    22021 agcgttaacg ctgtgccatg aagcaacagc gaggagtgta gtcgcaaatg gcgaaagaaa
    22081 aaaaacgtgg ctttttttcc tggctgggct ttggtcaaaa agagcagacc ccggaaaaag
    22141 agacagaagt tcagaatgaa caaacggttg tagaagaaat cgttcaggcg caagagcctg
    22201 tgaaggcttc tgaacaagcc gtcgaagagc agccgcaggc gcatactgag gccgaggcgg
    22261 aaacttttgc tgccgacgtt gtggaagtca ctgaacaggt tgctgaaagt gaaaaagcgc
    22321 agcctgaagc ggaagtcgtt gcacaaccgg aaccggtcgt agaagaaacg ccggagccag
    22381 tggctatcga acgtgaagag ctgccgttgc cggaagacgt caacgccgaa gaggtttcgc
    22441 cagaagagtg gcaggctgaa gcggaaaccg tagagattgt cgaagcggcg gaagaagaag
    22501 cggctaaaga agaaatcacc gacgaagagc tggaagcaca ggcgctggct gccgaagcgg
    22561 cagaagaagc ggtgatagtg gttcctccgg cagaagaaga gcagccggtt gaagaaattg
    22621 cccaggagca ggaaaaaccg accaaagaag gtttcttcgc gcgcctgaaa cgcagcctgt
    22681 taaaaaccaa agaaaatctc ggttccggat ttatcagcct gttccgcggt aaaaaaatcg
    22741 acgatgatct gtttgaagag ctggaagaac agctgttgat cgccgatgtg ggcgtggaaa
    22801 ccacacgtaa aatcatcacc aatctgacgg aaggcgcatc ccgcaagcag cttcgtgatg
    22861 ccgaggccct ctatggcctg ctgaaagaag agatgggcga gattctggcg aaagtcgatg
    22921 agccgctgaa tgttgaaggt aaaacgccgt tcgtgatcct gatggtaggc gtcaacggtg
    22981 tgggtaaaac cacgacgatt ggtaagctgg cgcgtcagtt tgagcagcag ggtaaatcgg
    23041 tgatgctggc ggcgggcgat actttccgtg cagcagcggt tgaacagctt caggtctggg
    23101 ggcagcgcaa caatattccg gtgattgccc agcataccgg tgcggattcc gcctctgtta
    23161 tcttcgacgc cattcaggcc gctaaagcgc gtaatatcga cgtcctgatt gccgataccg
    23221 ccggacgcct gcagaacaaa tcgcacctga tggaagagtt gaagaaaatc gtccgcgtga
    23281 tgaagaaact cgacgttgaa gcgccgcatg aagttatgct gactatcgat gccagcaccg
    23341 ggcagaacgc ggtaagccag gccaaactgt tccatgaagc tgttggctta accggcatca
    23401 cgctaacgaa actggacggc acggcgaaag gcggggtaat tttctcggtg gctgaccagt
    23461 ttggtatccc tatccgctac attggtgtcg gcgaacgtat tgaggatttg cgtccgttta
    23521 aggcggacga ctttatagag gcactttttg cccgagagga ttaacaatga ttcgctttga
    23581 acatgtcagc aaggcttatc tcggtgggag acaggcgctg cagggcgtta cgttccatat
    23641 gcagccgggt gagatggcgt ttctgaccgg tcattccggc gcagggaaaa gtaccctcct
    23701 gaagctgatc tgtgggattg agcggcccag cgccgggaaa atctggttta gcggccatga
    23761 catcacgcgt ctgaaaaacc gtgaagttcc gtttctgcgc cgccagattg gcatgatttt
    23821 ccaggatcac catctactga tggaccgtac tgtctacgat aacgtggcaa tcccgctgat
    23881 tatcgccggt gccagcggtg acgatattcg tcgccgggtg tcggcggcac tggataaagt
    23941 cgggctactg gacaaagcga agaacttccc tattcagctt tctggcggtg aacaacagcg
    24001 tgttggcatt gcccgcgcgg tggtgaacaa gcccgcggta ctgctggcgg acgaaccgac
    24061 tggtaacctg gacgacgcgt tgtcggaagg gattctacgt ctgtttgaag agtttaaccg
    24121 cgttggggta accgtattga tggcaacgca cgacatcaac ctgatctcgc ggcgttccta
    24181 tcgcatgctc accctgagcg atggtcactt gcatggaggc gtgggccatg aataagcgcg
    24241 atgcaatcaa tcatattcgg cagtttggcg ggcgtctgga tcgcttccgt aaatcggtcg
    24301 gcggctctgg cgacggcggt cgtaacgcgc caaaacgcgc gaaatcctcg ccaaaaccgg
    24361 taaatcgcaa aaccaacgtt ttcaacgaac aggtgcgcta tgccttccac ggcgcattgc
    24421 aggatctgaa aagcaaaccg ttcgccacgt ttttaacggt gatggttatc gccatctccc
    24481 tgacgctgcc cagcgtctgt tatatggtgt acaaaaacgt taaccaggcg gcgacgcaat
    24541 attatccgtc accgcaaatc actgtttact tgcaaaaaac gctggacgat gacgccgcag
    24601 caggcgtggt agcacagcta caggccgagc agggcgtgga gaaagtgaac tatctttctc
    24661 gtgaagacgc actgggcgag ttccgtaact ggtctggttt tggtggtgcg ctggatatgc
    24721 tggaagaaaa cccgcttccg gcagtggcgg tggtgatccc gaaactcgat tttcagggga
    24781 cggaatcact gaatacgctg cgtgatcgta tcacccagat taacggcatt gacgaagtgc
    24841 ggatggatga cagctggttt gcccgtctgg cggcgttgac cgggctggtc gggcgcgttt
    24901 ccgcgatgat cggcgtgttg atggtggcag cagtgttcct cgtcatcggc aacagtgtgc
    24961 gtctgagcat ctttgctcgc cgtgactcca ttaacgtgca gaaactgatt ggtgcgacgg
    25021 atggtttcat cctgcgcccg ttcctgtacg gaggcgcatt gctgggattt tctggcgcat
    25081 tgctgtcatt aattttgtca gaaattctgg tactgcgttt gtcatcggcg gttgcagaag
    25141 tggcacaggt tttcggaacg aagtttgata tcaatggctt atcgttcgat gaatgcctgc
    25201 tgttgctgct ggtttgctct atgattggct gggtggcagc gtggcttgcc acggtacaac
    25261 atttacgcca ctttacgcct gaataataaa agcgtgttat actctttccc tgcaatgggt
    25321 tccgtagcag ggaaagagac cccgttgtct cttcccggta tttcatctct atgtcacatt
    25381 ttgtgcgtaa tttattcaca agcttgcatt gaacttgtgg ataaaatcac ggtctgataa
    25441 aacagtgaat gataacctcg ttgctcttaa gctctggcac agttgttgct accactgaag
    25501 cgccagaaga tatcgattga gaggatttga atgactgaca aaatgcaaag tttagcttta
    25561 gccccagttg gcaacctgga ttcctacatc cgggcagcta acgcgtggcc gatgttgtcg
    25621 gctgacgagg agcgggcgct ggctgaaaag ctgcattacc atggcgatct ggaagcagct
    25681 aaaacgctga tcctgtctca cctgcggttt gttgttcata ttgctcgtaa ttatgcgggc
    25741 tatggcctgc cacaggcgga tttgattcag gaaggtaaca tcggcctgat gaaagcagtg
    25801 cgccgtttca acccggaagt gggtgtgcgc ctggtctcct tcgccgtcca ctggatcaaa
    25861 gcagagatcc acgaatacgt tctgcgtaac tggcgtatcg tcaaagttgc gactaccaaa
    25921 gcgcagcgca aactgttctt caacctgcgt aaaactaagc agcgtctggg ctggtttaac
    25981 caggatgaag tcgaaatggt ggcccgtgaa ctgggcgtaa ccagcaaaga cgttcgtgag
    26041 atggaatcac gtatggcggc acaggacatg acctttgacc tgtcttccga cgacgattcc
    26101 gacagccagc cgatggcacc ggtgctctat ctgcaggata aatcatctaa ctttgccgac
    26161 ggcatcgaag atgataactg ggaagagcag gcggcaaacc gtctgaccga cgcgatgcag
    26221 ggtctggacg aacgcagcca ggacatcatc cgtgcgcgct ggctggacga agacaacaag
    26281 tccacgttgc aggaactggc tgaccgttac ggtgtttccg ccgagcgtgt gcgccagctg
    26341 gaaaagaacg cgatgaaaaa acttcgcgct gccattgaag cgtaatttct actactaagc
    26401 agagaaccct ggatgagaat ccggggtttt tgttttttgg gcctctacaa taatcaattc
    26461 cccctccggc aaaacgtcaa tccccacgca gattgttaat aaactgtcaa aatagctatt
    26521 ccaatatcat aaaaatgggg tatgttttag cagagtatgc tgctaaagca cgggtagtca
    26581 tgcataaaac gaaataaagt gctgaatcac aacaccacaa cacacgtaat aaccagaaga
    26641 atggggattc tcaggatgaa cataaagggt aaagcgttac tggcaggatg tatcgcgctg
    26701 gcattcagca atatggctct ggcagaagat attaaagtcg cggtcgtggg cgcaatgtcc
    26761 ggtccggttg cgcagtacgg tgaccaggag tttaccggcg cagagcaggc ggttgcggat
    26821 atcaacgcta aaggcggcat taaaggcaac aaactgcaaa tcgtaaaata tgacgatgcc
    26881 tgtgacccga aacaggcggt tgcggtggcg aacaaagtcg ttaacgacgg cattaaatat
    26941 gtgattggtc acctctgttc ttcatcaacg cagcctgcgt ctgacatcta cgaagacgaa
    27001 ggcattttaa tgatcacccc agcggcaacc gcgccggagc tgaccgcccg tggctatcag
    27061 ctgatcctgc gcaccaccgg cctggactcc gaccaggggc cgacggcggc gaaatatatt
    27121 cttgagaaag tgaaaccgca gcgtattgct atcgttcacg acaaacagca atacggcgaa
    27181 ggtctggcgc gagcggtgca ggacggcctg aagaaaggca atgctaacgt ggtgttcttt
    27241 gatggtatca ccgccgggga aaaagatttc tcaacgctgg tggcccgtct gaaaaaagag
    27301 aatatcgact tcgtttacta cggcggttat cacccggaaa tggggcaaat cctgcgtcag
    27361 gcacgcgcgg cagggctgaa aactcagttt atggggccgg aaggtgtggc taacgtttcg
    27421 ctgtctaaca ttgcgggcga atcagcggaa gggctgctgg tgaccaagcc gaagaactac
    27481 gatcaggttc cggcgaacaa acccattgtt gacgccatca aagcgaaaaa acaggaccca
    27541 agcggcgcat tcgtttggac cacctacgcc gcgctgcaat ctttgcaggc gggcctgaat
    27601 cagtctgacg atccggctga gatcgccaaa tacctgaaag cgaactccgt ggataccgtg
    27661 atgggtccgc tgacgtggga tgagaaaggt gatctgaaag gctttgagtt cggcgtattt
    27721 gactggcacg ccaacggcac ggcgaccgat gcgaagtaat cattaatcgg caactttggg
    27781 ttgccgccaa attgctaata tcgagtacgt tgcttcatgc cggatgcggc gtaaacgcct
    27841 tatccggcct acaagatcca aagaaatcag taaattgcaa cacacattgt aggcctgata
    27901 agcgtagcgc atcaggcaat ttctcgttgg tcatcacttt tgaaatcaac ttgacgatta
    27961 acgcttctcc cagccgccct gttgcgccgt aaaccccagc gcctgcataa acgccgtcat
    28021 cacaccgcga tcttccacgc ctgcatccgc catccaccag catgaaacgg caggattgtt
    28081 acgcaaaacc tcttccagca gatattgccc cacaccgcga cggcgggtga cttcccgcac
    28141 gcgcagggaa tccagtgctc cctcggtgcc gcttaaggtt acccgcacgg ccgctagcag
    28201 gcgctcgtta aaacgcgcgg cgtagatgcg gtggttatcg tcaacctgta aagaggaagg
    28261 ggaatactcc ggccagatct tttgcaggtc aatccggtcc tggtcgctaa agttttctaa
    28321 tcgaatgatg gtcagcttca tgggtaaccc gtgtaaatca caaaagtgga accagtgtag
    28381 cgaaataatt taatcggagg ctttctcttt tttatttctt ttggcaggtg attaattttt
    28441 taacagcaat aattacaaag ttaaaacatt atagaatgaa a
//
LOCUS       DAPHDW010000049        28245 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.049, whole genome shotgun sequence.
ACCESSION   DAPHDW010000049 DAPHDW010000000
VERSION     DAPHDW010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28245
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.049"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RH057_002118"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RH057_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3128481.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="RH057_002119"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="RH057_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HDV3128482.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRMGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="RH057_002120"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="RH057_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HDV3128483.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="RH057_002121"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="RH057_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HDV3128484.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="RH057_002122"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="RH057_002122"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HDV3128485.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQISNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="RH057_002123"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="RH057_002123"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HDV3128486.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLTVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLGLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="RH057_002124"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="RH057_002124"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HDV3128487.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIVEGHELSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="RH057_002125"
     CDS             complement(4598..5320)
                     /locus_tag="RH057_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3128488.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="RH057_002126"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="RH057_002126"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV3128489.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKAAFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="RH057_002127"
     CDS             6520..6738
                     /locus_tag="RH057_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HDV3128490.1"
                     /translation="MQDLSLETRLAELESRLAFQEITIEELNVTVTAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="RH057_002128"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="RH057_002128"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HDV3128491.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="RH057_002129"
     CDS             complement(7472..7672)
                     /locus_tag="RH057_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HDV3128492.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVRKDEQVIGIFHPD"
     gene            complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="RH057_002130"
     CDS             complement(7682..9487)
                     /gene="kefB"
                     /locus_tag="RH057_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
                     /protein_id="HDV3128493.1"
                     /translation="MEGSDFLLAGVLFLFAAVAAVPLASRLGIGAVLGYLLAGIAIGP
                     WGLGFISDVDEILHFSELGVVFLMFIIGLELNPSKLWQLRRSIFGVGAAQVLLSAALL
                     AGLLMLTDFAWQAAVVGGIGLAMSSTAMALQLMREKGMNRSESGQLGFSVLLFQDLAV
                     IPALALVPLLAGSADEHFDWMKIGMKVLAFVGMLIGGRYLLRPVFRFIAASGVREVFT
                     AATLLLVLGSALFMDALGLSMALGTFIAGVLLAESEYRHELETAIDPFKGLLLGLFFI
                     SVGMSLNLGVLYTHLLWVVISVVVLVAVKILVLYLLARLYGVRSSERMQFAGVLSQGG
                     EFAFVLFSTASSQRLFQGDQMALLLVTVTLSMMTTPLLMKLVDKWLSRQFNGPEEEDE
                     KPWVNDDKPQVIVVGFGRFGQVIGRLLMANKMRITVLERDISAVNLMRKYGYKVYYGD
                     ATQVDLLRSAGAEAAESIVITCNEPEDTMKLVEICQQHFPHLHILARARGRVEAHELL
                     QAGVTQFSRETFSSALELGRKTLVTLGMHPHQAQRAQLHFRRLDMRMLRELIPMHADT
                     VQISRAREARRELEEIFQREMQQERRQLDGWDEFE"
     gene            complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="RH057_002131"
     CDS             complement(9487..10038)
                     /gene="kefG"
                     /locus_tag="RH057_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312229.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HDV3128494.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRSVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     QELASHARAYGDWLANPLSPGGR"
     gene            10169..12082
                     /locus_tag="RH057_002132"
     CDS             10169..12082
                     /locus_tag="RH057_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3128495.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHGKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFQGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQADEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12082..13104
                     /locus_tag="RH057_002133"
     CDS             12082..13104
                     /locus_tag="RH057_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV3128496.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRKVKFT
                     PYWQRLELPDGDFVDLAWSEAPAQARHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPPQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13098..13316
                     /locus_tag="RH057_002134"
     CDS             13098..13316
                     /locus_tag="RH057_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HDV3128497.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13370..14239
                     /locus_tag="RH057_002135"
     CDS             13370..14239
                     /locus_tag="RH057_002135"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HDV3128498.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14294..14698)
                     /locus_tag="RH057_002136"
     CDS             complement(14294..14698)
                     /locus_tag="RH057_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HDV3128499.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            15000..15632
                     /gene="crp"
                     /locus_tag="RH057_002137"
     CDS             15000..15632
                     /gene="crp"
                     /locus_tag="RH057_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HDV3128500.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15683..17773
                     /locus_tag="RH057_002138"
     CDS             15683..17773
                     /locus_tag="RH057_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312236.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV3128501.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKRLIIGASLFATCSLLTQLLLAKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTALHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPEGYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="RH057_002139"
     CDS             complement(17840..19060)
                     /gene="argD/dapC"
                     /locus_tag="RH057_002139"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HDV3128502.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYMHYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLSCAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPQFKGRARDFLYAGAGAGVMVLNAGPDVMRFAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="RH057_002140"
     CDS             complement(19146..19709)
                     /gene="pabA"
                     /locus_tag="RH057_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HDV3128503.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHNGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHR"
     gene            complement(19741..20343)
                     /locus_tag="RH057_002141"
     CDS             complement(19741..20343)
                     /locus_tag="RH057_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HDV3128504.1"
                     /translation="MSDKFGEGRDPYLYPGLDIMRNRLNIHQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20333..20500)
                     /locus_tag="RH057_002142"
     CDS             complement(20333..20500)
                     /locus_tag="RH057_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HDV3128505.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="RH057_002143"
                     /gene_synonym="rotA"
     CDS             complement(20605..21177)
                     /gene="ppiA"
                     /locus_tag="RH057_002143"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HDV3128506.1"
                     /translation="MFKSTLAAMAAVFALSALSPAAMAAKGDPHVLLTTSAGNIELEL
                     DKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKNEAD
                     NGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMDVAD
                     KISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21448..22629
                     /gene="tsgA"
                     /locus_tag="RH057_002144"
     CDS             21448..22629
                     /gene="tsgA"
                     /locus_tag="RH057_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HDV3128507.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSAA
                     MFILGVVSGITMSIGTFLVTQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKHAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPAHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGPIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22891..25434
                     /gene="nirB"
                     /locus_tag="RH057_002145"
     CDS             22891..25434
                     /gene="nirB"
                     /locus_tag="RH057_002145"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HDV3128508.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25431..25757
                     /gene="nirD"
                     /locus_tag="RH057_002146"
     CDS             25431..25757
                     /gene="nirD"
                     /locus_tag="RH057_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HDV3128509.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDEQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25884..26690
                     /gene="nirC"
                     /locus_tag="RH057_002147"
     CDS             25884..26690
                     /gene="nirC"
                     /locus_tag="RH057_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HDV3128510.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26709..28082
                     /gene="cobA"
                     /locus_tag="RH057_002148"
     CDS             26709..28082
                     /gene="cobA"
                     /locus_tag="RH057_002148"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV3128511.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDALNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
BASE COUNT         6829 a         7574 c         7146 g         6696 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gataatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt cagaccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcatcatcac ttgcgtgacg ctcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcgttccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccatgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaagc gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcgaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagaccca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggtcag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcacttgcc
     4441 ggtgaggtca attggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 tcatggcctt ctactatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctggaaat
     4621 caccgctttt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgcg tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 accagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc gactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttcttttag agagttttcc
     6121 atgtaacgac ccagcgaggc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gctgctttgc tgtcagcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agcagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 acacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tgaccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaggaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcaac cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccacgacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc accaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gcgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tggtgtccgc
     7561 acttaacgca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcaa taaaacgttt tcggattgcc atctttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcggcat
     7801 gcattgggat gagctctcgc agcatccgca tatccaggcg gcgaaaatgc agttgcgctc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 cgtatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgtggtcatc atggaaagcg
     8401 tcaccgtcac cagcaacaac gccatctggt cgccctggaa taagcgttgt gaagaagcgg
     8461 tagaaaagag gacaaaggca aactcaccac cctgactcaa cacgccagca aactgcatcc
     8521 gctctgagct acgcacgcca tacaatcgcg ccagcagata cagcacgaga attttcaccg
     8581 ccaccagcac aaccacgctt atcactaccc acaacagatg ggtataaagc accccgaggt
     8641 tgagtgacat gccgacagag ataaagaaca aaccgagcag caagcctttg aagggatcga
     8701 tagccgtttc cagttcatgg cgatattcac tttccgccag cagcacgccc gcaataaatg
     8761 taccgagcgc catcgacagc cccagcgcat ccataaacaa tgcggaaccc aacaccagca
     8821 gcagcgtcgc ggcggtgaac acttcccgca cgccagaagc tgcaataaag cggaataccg
     8881 gacgcagtaa atagcgccca ccaatcagca tgccgacaaa cgccagcacc ttcatgccga
     8941 tcttcatcca gtcgaaatgt tcgtctgccg accccgccaa caacggcact aacgccagtg
     9001 ctgggattac tgccagatcc tgaaacagca gaaccgaaaa cccgagctgg ccggattcgc
     9061 tgcgattcat ccctttctca cgcatcaatt gcaatgccat tgcagttgaa gacatcgcaa
     9121 ggccaatgcc accgaccacc gccgcctgcc aggcgaaatc cgtcagcatc aataatcccg
     9181 ccagcaacgc cgcgcttaac agcacctgtg ccgcgcctac gccaaaaatc gaacgccgca
     9241 gttgccaaag tttggaggga ttcaactcaa ggccgatgat aaacatcagg aataccacgc
     9301 cgagttccga aaagtggagg atctcatcga cgtcgctaat aaaccccagc ccccacgggc
     9361 caattgcaat ccctgccagc aaatatccca acacagcgcc aatacccagc cgcgatgcca
     9421 gcggcaccgc agccaccgcc gcgaagagaa acagcactcc tgcgagtaaa aaatcggaac
     9481 cttccatcag cggcctcctg gagacagcgg atttgccagc cagtcaccgt aggctctggc
     9541 gtggctcgcc agctcctgtg cgctttgccg tctcgcccag taaataatga tgggacttaa
     9601 ccaatgcatc cggcacatgc ccgccgccag ttcaaagggg cgcagcacat cgctcatcgg
     9661 gtagcgattc agcgcgtcat aacggtaagc actttccggc tcgccggtgg taatcacgct
     9721 acgccagtac tttcccgcca gttggtttcc tcccggcccg ctggcaaaac cacgacttaa
     9781 tacccggtcc agccactctt tcagtagcgc cgggcagcta taggtataaa gaggatgctg
     9841 aaagacaatc acctcgtgct cgcgcagtaa tgcctgctca cgggggatat caataaaaaa
     9901 atcgggatag tgcgcgtaaa ggtcgtgcac ggtaacattg ctgagctgcg tggccggttt
     9961 aagcagtacc cggtttgcca ccgagtcctg agattccgga tgggcataca gcagcaaaac
    10021 tttcgctggc tgagacatca tccccctccc ggtatggttt ttgtgtatag tcgctgtttt
    10081 gggctaccat tgcgcccggt gcggcagctc gcccatacat tacattatca taatgataag
    10141 ttaacatagt ctgaacatac ggcaccttat gattgttttc tcctcgttac aaattcgtcg
    10201 cggcgtgcgc gtcctgctgg ataatgccac cgccaccatc aacccagggc agaaagtcgg
    10261 cctggtgggt aaaaacggct gtggtaaatc taccctgctg gcattgctga aaaatgaaat
    10321 cagcgccgac ggcggcagct acacctttcc gggaagctgg caactggcgt gggtgaatca
    10381 ggaaacgccg gcgttaccgc aagcggcgct ggaatatgtc attgacggcg accgtgaata
    10441 tcgtcaacta gaagcgcagc tacacgacgc caacgaacgt aacgacgggc acgccattgc
    10501 gaccattcat ggcaagctgg atgctattga cgcatggagt attcgctccc gtgctgccag
    10561 cctgctgcac ggcctcggtt tcagcaatga acaactggag cgcccggtaa gtgatttttc
    10621 cgggggctgg cgtatgcgtc ttaaccttgc ccaggcgctg atttgccgtt cagacttgct
    10681 gctgctcgac gaaccgacta accacctcga tctcgatgcc gttatctggc tggaaaaatg
    10741 gttgaagagc tatcagggca cgctgatcct gatctctcac gaccgcgact tcctcgatcc
    10801 gatcgttgat aaaattattc atatcgaaca acaaagcatg ttcgagtaca ccggtaacta
    10861 cagttcgttt gaagtacagc gcgccacccg tctggcgcag caacaagcga tgtatgaaag
    10921 ccagcaggaa cgcgtagcgc atctgcaaag ttatatcgac cgtttccgtg ccaaagccac
    10981 caaagcgaag caggcccaga gccgcattaa gatgctcgag cgtatggagc tgattgcccc
    11041 cgcgcacgtc gacaacccgt tccgctttag cttccgcgcg ccggaaagcc tgccaaatcc
    11101 gttactgaag atggaaaaag tcagcgcggg ctatggcgat cgcattattc tcgactcgat
    11161 taaactgaac ctggtgcccg gctcgcgcat tggtctgtta ggccgcaacg gcgcgggtaa
    11221 atcgacatta atcaaactgt tagccggtga acttgcgcca gtcagcggtg aaattggtct
    11281 ggcgaaaggg atcaagctcg gctacttcgc ccagcatcaa cttgaatacc tgcgcgccga
    11341 cgaatcacct attcaacatc tggcacgttt agcgccgcag gagctggaac aaaaactgcg
    11401 tgactacctc ggcggctttg gtttccaggg cgataaagta accgaagaaa cgcgccgctt
    11461 ctcaggtggg gaaaaagccc gcctggtgct ggcattaatt gtctggcagc gtccgaatct
    11521 gctgctgctc gacgaaccga ctaaccacct tgacctcgac atgcgtcagg cactcaccga
    11581 agcattaatc gagttcgaag gcgcgctggt tgtcgtttcg cacgaccgtc atttgctgcg
    11641 ttccaccact gacgatctct acctggttca cgatcgtaaa gtcgaaccgt tcgacggcga
    11701 tctggaagat tatcaacagt ggttgagcga cgtacaaaag caggaaaacc aggccgacga
    11761 agcgccaaaa gagaacgcga acagcgccca ggcacgtaaa gatcagaagc gtcgggaagc
    11821 ggagttgcgt gcgcaaaccc agccactgcg taaagagatt gcccgtctgg aaaaagagat
    11881 ggagaagctg aacgcgcaac tggcgcaggc agaagagaaa ctcggcgaca gcgaactgta
    11941 tgaccagagc cgtaaagcgg agttgaccgc ctgcctgcaa cagcaagcca gcgccaaatc
    12001 cggcctggaa gagtgcgaaa tggcatggct ggaagcccag gagcagcttg agcagatgtt
    12061 gctggaaggc caaagcaact gatggcgcag ataacgacga ccgatgccaa tgaattcagc
    12121 agcagtgctg aattcacccc tatgcgcggc tttagcaatt gtcatctgca aaccatgctg
    12181 ccgcgtctgt ttcgtcgcaa ggtgaaattc accccgtact ggcagcggct ggagttgccc
    12241 gacggcgatt ttgtcgatct cgcgtggagt gaagcccctg cacaggctcg gcataaaccg
    12301 cgtttagtgg tatttcacgg gctggaaggc agtctcaata gcccttacgc ccacggtctg
    12361 gttgaggcgg cgcaaaagcg cggctggctg ggcgtggtga tgcattttcg cggatgcagc
    12421 ggtgaaccaa accgtatgca ccgcatttac cattcgggcg aaaccgaaga cgccagttgg
    12481 tttttacgct ggctgcaacg cgaatttggt catgcgccaa cggctgccgt cggctattcg
    12541 ctcggcggta atatgctggc ctgtttgctg gcaaaggaag gcaatgatct cccggttgat
    12601 gcggcggtga ttgtctctgc gccgtttatg ctggaagcct gtagctatca tatggaaaag
    12661 ggcttttccc gcgtttatca gcgttacttg ctgaacctgt taaaagccaa tgccgcgcgc
    12721 aagctggcag cctaccccgg aacgctgccg attaatctcg cgcagttaaa atcggtacgt
    12781 cgcatccgtg aatttgacga tctcatcacc gccagaattc acggctacgc cgacgctatc
    12841 gactattatc gtcagtgtag cgccatgccg atgctgaacc ggatcgccaa accgacgctg
    12901 attattcacg ccaaagacga tccgtttatg gatcatcagg tgatcccgaa accggaaagt
    12961 ctccccccgc aggtggagta tcaactgact gaacatggcg gtcatgttgg ctttattggc
    13021 ggtacattac ttcatccgca aatgtggctg gagtcacgca ttcctgactg gttaacaacg
    13081 tatctggagg cgaaatcatg ttgattccgt ggcaagacct ctcccccgaa acgctggaaa
    13141 atttaattga aagctttgtg ttacgtgaag gcaccgatta tggtgaacat gagcgtacac
    13201 ttgaacagaa agtcgccgac gtcaaacgcc agctacagtg cggagaagcg gtgctggtat
    13261 ggtcggagct gcacgaaacg gtcaatatta tgccgcgcag tcagtttcgc gaataacctg
    13321 caaccgtcgg cggacgagat aataatgata gctaagtcat ggagttacta tgtctgccaa
    13381 acatccggtc attgcggtaa caggatccag cggcgcgggg accaccacca ccagcctcgc
    13441 gtttcgtaaa atattcgcgc agttaaatct gcatgcagct gaggtggaag gcgacagttt
    13501 tcaccgttac acccgcccgg aaatggacat ggcgatccgc aaagcgcgcg acgccgggcg
    13561 gcatatcagc tacttcggcc ccgaagcgaa cgacttcggc ctgctggaac aaaccttcat
    13621 tgaatacggt cagagcggca aagggaaatc tcgcaaatat ctgcatacct acgatgaagc
    13681 cgtaccgtgg aatcaggtac cgggaacatt caccccctgg caacctttac cagaacccac
    13741 tgatgtactg ttttatgaag gtttacacgg cggcgtagtc acgccacagc ataacgttgc
    13801 gcagcatgtg gacttactgg tcggcgtggt gcctatcgtt aaccttgagt ggattcaaaa
    13861 actgatccgc gacaccagcg agcgcgggca ctcacgagaa gcagtgatgg actcagtagt
    13921 gcgttcaatg gaagactata tcaactacat cacaccgcag ttttcccgca cccatcttaa
    13981 cttccagcgc gttcccaccg tcgacacttc aaacccgttc gcggcaaaag gtatcccgtc
    14041 gctggatgaa agctttgtgg tgatccattt tcgtaatctg gaagggatcg atttcccctg
    14101 gctgctggcg atgttgcaag gctcattcat ttcccacatc aatacgttag tggtaccggg
    14161 cggcaaaatg gggctggcaa tggaattaat tatgctgccg ctggtgcaac gattgatgga
    14221 aggaaagaaa atcgagtaac tctgctatta cgccggataa aatactatcc ggcttcacaa
    14281 cgggatagtt aaatcacgcg gcaaccactt catacgagtg agtaatattc accgcttttt
    14341 ccagcatcaa cgccactgaa caatatttct cagcagagag atcaaccgca cgcgcaaccg
    14401 ctgcgtcttt caggtcgcga ccggtgacga taaaatgcag attaatgtgc gtaaacaggc
    14461 gtggtgcctc ttcgcggcgt tcagaggtca attttacttc acaatcgacc acatcctgac
    14521 gccctttttg caggatcgaa accacatcga tggcactgca accacccgcc gccatcaaca
    14581 ccatttccat cggacttggt gctttatcgc ctgagttgcc gtccattaaa atctggtgac
    14641 cagaggcgga ttcgcccaga aaagttaacc cttcgaccca cttcactcgc gcttgcattt
    14701 ttgctactcc actgcgtcaa ttttcctgac agagtacgcg tactaaccaa atcgcgcaac
    14761 ggaaggcgac ctgggtcatg ctgaagcgag acaccaggag acacaaagcg aaagctatgc
    14821 taaaacagtc aggatgctac agtaatacat tgatgtactg catgtatgca aaggacgtca
    14881 cattaccgtg cagtacagtt gatagcccct tcccaggtag cgggaagcat atttcggcaa
    14941 tccagagaca gcggcgttat ctggctctgg agaaagctta taacagagga taaccgcgca
    15001 tggtgcttgg caaaccgcaa acagacccga ctctcgaatg gttcttgtct cattgccaca
    15061 ttcataagta cccatccaag agcacgctta ttcaccaggg tgaaaaagcg gaaacgctgt
    15121 actacatcgt taaaggctct gtggcagtgc tgatcaaaga cgaagagggt aaagaaatga
    15181 tcctctccta tctgaatcag ggtgatttta ttggcgaact gggcctgttt gaagagggcc
    15241 aggaacgtag cgcatgggta cgtgcgaaaa ccgcctgtga agtggctgaa atttcgtaca
    15301 aaaaatttcg ccaattgatt caggtaaacc cggacattct gatgcgtctg tctgcacaga
    15361 tggcgcgtcg tctgcaagtc acttcagaga aagtgggcaa cctggcgttc ctcgacgtga
    15421 cgggccgcat tgcacagact ctgctgaacc tggcaaaaca accagatgct atgactcacc
    15481 cggacggtat gcaaatcaaa attacccgtc aggaaatcgg tcagattgtc ggctgttctc
    15541 gtgaaaccgt gggacgcatt ctgaagatgc tggaagatca gaacctgatc tccgcacacg
    15601 gtaaaaccat cgtcgtttac ggcactcgtt aatcccgtcg gagtggcgcg ttacctggta
    15661 gcgcgccatt ttgtttcccc cgatgtggcg cagactgatt tatcaccccg atatcaacta
    15721 tgcacttcga caaacgctgg tgctatgttt gcccgtggcc gttgggttaa tgcttggcga
    15781 attacgattc ggtctgctct tctctctcgt tcctgcctgt tgcaatattg cgggccttga
    15841 tacgcctcat aaacgttttt tcaaacgctt aatcattggt gcgtcgctgt ttgccacctg
    15901 tagcttactg acacagctgc tactggcaaa agatgttccc ctgccctttt tgctgaccgg
    15961 attaacgctg gtacttggcg tcactgctga gttggggcca ttgcacgcaa aattgcttcc
    16021 cgcatcgctg ctggcagcca tttttaccct cagtctggcg ggatacatgc cggtctggga
    16081 accgttgctc atctatgcgt tgggcactct ctggtacgga ttgtttaact ggttttggtt
    16141 ctggatctgg cgcgaacaac cgctgcgcga gtcactaagt ctgctgtacc gtgaactggc
    16201 agattattgt gaagccaaat acagcctgct tacccagcac accgatcctg aaaaagcgct
    16261 gccgccgctg ctggtgcgcc agcaaaaagc ggtcgatcta attacccagt gctatcagca
    16321 aatgcatatg ctttccgcgc aaaataatac tgactacaag cggatgctgc gtattttcca
    16381 ggaggcgctg gatttacagg aacatatttc ggtcagtttg catcagccgg aagaggtgca
    16441 aaagctggtc gagcgtagcc atgcggaaga agttatccgc tggaatgcgc aaaccgtcgc
    16501 cgctcgcctg cgcgtgctgg ctgatgacat tctttaccat cgcctgccaa cgcgttttac
    16561 gatggaaaag caaattggcg cactggaaaa aatcgcccgc cagcatccgg ataatccggt
    16621 tgggcaattc tgctactggc atttcagccg catcgcccgc gtgctgcgca cccaaaaacc
    16681 gctctatgcc cgtgacttac tggccgataa acagcggcga atgccattac ttccggcgct
    16741 gaaaagttat ctgtcactaa agtctccggc gctacgcaat gccggacgac tcagtgtgat
    16801 gttaagcgtt gccagcctga tgggcaccgc gctgcatctg ccgaagtcgt actggatcct
    16861 gatgacggta ttgctggtga cacaaaatgg ctatggcgca acccgtctga ggattgtgaa
    16921 tcgctccgtg ggaaccgtgg tcgggttaat cattgcgggc gtggcgctgc actttaaaat
    16981 tcccgaaggt tacaccctga cgttgatgct gattaccacc ctcgccagct acctgatatt
    17041 gcgcaaaaac tacggctggg cgacggtcgg ttttactatt accgcagtgt ataccctgca
    17101 actattgtgg ttgaacggcg agcaatacat ccttccgcgt cttatcgata ccattattgg
    17161 ttgtttaatt gctttcggcg gtactgtctg gctgtggccg cagtggcaga gcgggttatt
    17221 gcgtaaaaac gcccatgacg ctttagaagc ctatcaggaa gcgattcgct tgattcttag
    17281 cgaggatccg caacctacgc cactggcctg gcagcgaatg cgggtaaatc aggcacataa
    17341 cactctgtat aactcattga atcaggcgat gcaggaaccg gcgtttaaca gccattatct
    17401 ggcagatatg aaactgtggg taacgcacag ccagtttatt gttgagcata ttaatgccat
    17461 gaccacgctg gcgcgggaac accgggcatt gccacctgaa ctggcacaag agtatttaca
    17521 gtcttgtgaa atcgccattc agcgttgtca gcagcgactg gagtatgacg aaccgggtag
    17581 ttctggcgat gccaatatca tggatgcgcc ggagatgcag ccgcacgaag gcgcggcagg
    17641 tacgctggag cagcatttac agcgggttat tggtcatctg aacaccatgc acaccatttc
    17701 gtcgatggca tggcgtcagc gaccgcatca cgggatttgg ctgagtcgca agttgcggga
    17761 ttcgaaggcg taatgcaggc taaaccatcg gataaggcat tcacgccgca tccgacatct
    17821 tttgcctgat gcttcgtatt tacgccccaa ccaccttcgc caccgcgtgg gcgaaacgtt
    17881 gcatcccttc atcgatatcc gcatcttcca ccaccagcga cggtgcaaaa cgcatcacat
    17941 ccggtccggc attcagcacc attacgccag cccctgcgcc cgcatacagg aaatcacgcg
    18001 cccgaccttt gaactgtggc ttaagctccg cgccaatcaa cagccccata ccgcgaatat
    18061 cgctaaacac atcgtactgc tgatcgatct tctgcagatg gtcaacaaaa cgctggcgtt
    18121 tcgcctgaat gccttccagc acttcagggg tattgatgat atcaaacgcc gcgcccgcta
    18181 ctgcacagga cagaggatta ccgccgtagg tggaaccgtg agaaccagga tgaaatgcag
    18241 aagcaatttc tgccgtggtc agcatggcgc taatcgggaa gccgccgcct aacgctttcg
    18301 cagaggtcag aatatccggc gtcacgccgt agtgcatgta agcaaacaaa tcgccggtcc
    18361 gccccatccc gcactgtact tcatcaaaca ccaataatgc ctgatgttga tcgcacagct
    18421 cgcgcaagcc ctgcaaaaac tctggcgtcg ctgccgtcac accgccctcg ccctggatcg
    18481 gctcaaccac caccgcacag gtgtgatcat ccatcaccgc tttcactgcg tggagatcgt
    18541 taaaaggaac gtggatgatg tctgccggtt ttggcccaaa gccgtcggaa tattttggct
    18601 gcccgcccac cgagacggta aacagcgagc gaccatgaaa agcgttatgg aaggcaataa
    18661 ttttggtttt gaacggacta tgacgcacac aggcgtaatg gcgtgccagt ttaaaggcgg
    18721 tttcgttagc ttccgtgccg gagttcataa acaccacgcg ttcggcaaac gtcgcctcaa
    18781 tcagtttgcg cccaagacgc agcgccggtt cattggtgaa aacgttactg atatgccaca
    18841 gagtttcgcc ctgggttttt aacgcgttca ccagcgcagg atggcaatgg cccaacgccg
    18901 taactgcaat gccacccgcg aaatcgacat actccttgcc ttgctgatcc cagattcggc
    18961 tgccctgacc ttttaccgga atgaactctg ccggagcata aatcggcagg atcacttcat
    19021 cgaaagtcgc gcgtgtaatt gctgtttgtt caattgccat ctcatgatca ccctgttacg
    19081 cataaataat tgtgaaatta taatcacaaa atatgcataa aaaatcacta aatggcaatc
    19141 agaaatcagc gatgcaggaa attagccagc agttgatgtc cttgttcgct aagaatactt
    19201 tctggatgga actgcacacc ttccagatcc cactggcgat ggcgaatccc cataatctcg
    19261 cgggtttcgc tccaggccgt cacgtcaaag cacgctggta atgagtcagg ttccaccacc
    19321 agcgaatggt agcgtgtcac ggtaagtgga tttgccagcc cccgaaatac gccctcaccg
    19381 ttatgtgtaa tcggcgaggt tttgccgtgc atgacctttg cggcgcgcac aactttaccg
    19441 ccaaatgcct gcgccattgc ctgatgaccg aggcagacgc caagaatcgg caagcgcccg
    19501 gcatagtggc gaataacgtc aagggagatc ccggcttcat ctggcgtaca ggggccaggt
    19561 gagatgacaa ttttttgtgg tttaagggcg tcgatatccg ccagcgtcaa cgcatcgttg
    19621 cgcttaacca gcacatccgc ccccagttca caaaagtact ggtagaggtt ccaggtaaaa
    19681 gaatcgtagt tatctataag caggatcatg gcggctccgg tacgaaagaa ccgcgctatt
    19741 ttactcagat tccccggctt cgcttaccac tttgctaaat atcatctgca gcgcggtgag
    19801 atcccccatt gccccactct gatttgcctg attccaggcc tctttttcga taccctgcca
    19861 gctcagttga tacccggcat gaatcgccag ttgctcgaag aagatccgct gtgccagacc
    19921 acttcccacc cggaagggat gcagcacgtt gatttcacaa tagtaatgcg ccagccgctc
    19981 gacgaacttc gctttctcca ggccaaccag ataaccttct tcctccagat cctgcatcag
    20041 ggcattgccc tctttttcga tataagcaaa gtggcagaac ggcgtatcgc cctgataaat
    20101 atcaacttca cgcagttgcc ccgcccagtc gaaaatatcc tgatacagct ggcgatggat
    20161 agtgcgcaaa tgcggtaaac cgcgcaccag cggaccaagc tcaatggtcg cagcacgcag
    20221 cgccgtcatt tcgtaagcgg cctgttccag ccgctgctgc tggtggatgt tcagccggtt
    20281 acgcatgata tcaaggccag gataaagata cggatcgcgc ccttcgccga atttatcgct
    20341 catagtgcct cctcagctct tcaaggcgcg ctaaagcctc tgcggcagta agagtgacta
    20401 aaggcatctc gacgccttca agacggcgac tggcctggaa attacgatta cgctgaagct
    20461 cccagagacg ggacttttgc ttatcggtga gtttcttcac ttgatgcctc cctgaacgtg
    20521 tccatttgcc acaagtataa gcggcaaatc cgggttacgc cgggagagca gaagcgcggg
    20581 caagattgcc cgcgcgaaaa atcattacgg caggacttta gcggaaagga taactaccgg
    20641 ttttgacggc acattctggt acggaccaac gtcatgagtc ggcacctggg aaatcttatc
    20701 ggcaacgtcc atgcctttca ccactttacc aaataccgcg taaccgaaat cacgctgacc
    20761 atggtcaagg aaggcgttat cggcaacgtt gataaagaac tggctggtgg cgctgtcttt
    20821 gtcagcggta cgcgccatcg cgatggtgcc acgcgtgttg cgcaggccgt tatcggcttc
    20881 atttttgatt ggcgggtttg gttttttctg ctgcatctgc tcggtgaaac cgccgccctg
    20941 aatcataaag ccaggaatga cgcggtgaaa ggtagtgttg ttataaaaac cgctgttcac
    21001 ataatcgaca aagttttgca cagacactgg cgctttttgt ttatccagct ccagttcgat
    21061 gttaccagct gaggttgtca acaatacgtg cgggtcccct ttcgctgcca ttgctgcggg
    21121 agaaagagca gaaagagcga aaacagcagc catcgccgcc agggtcgatt tgaacatgag
    21181 atttccttaa caaacagagc agaaaaaaag caagtgaaga gattctaaat agccttcaag
    21241 aacaaggcca tccctttacc taattttacg tatctgaaat atatgtaacc tgacgatcac
    21301 tttttattcc gtgatcaaaa tcacctctta aaatgcaatt tagcaaccga ttgcaataaa
    21361 acatttaaac agatcacaaa atcacctaaa atcgcccgct cgcggcatat cccgctatgc
    21421 cattttattt ttgaacgcag gccaatcatg actaacagca atcgcatcaa gctcacatgg
    21481 attagctttc tctcctacgc actgactggt gcgttggtta ttgtcaccgg gatggtgatg
    21541 ggaaatatcg ccgattattt caatctgcct gtttccagta tgagtaacac cttcaccttc
    21601 ctcaacgccg gcattttaat ctctatcttc ctcaacgcct ggctgatgga aatcgtcccg
    21661 ttgaaaacgc agttacgttt tggctttctc ctgatggtgc tggcggttgc cggtttgatg
    21721 ttcagccaca gcctggcgct gttctcggcg gcgatgttca ttctcggggt ggtcagcggc
    21781 atcaccatgt cgattggtac attcctggta acacaaatgt atgaagggcg tcagcgcggt
    21841 tcccgcctgt tatttaccga ctccttcttc agtatggctg ggatgatttt cccaatgatc
    21901 gccgcgtttc tactggcgcg cagcattgag tggtactggg tttatgcctg catcgggctg
    21961 gtgtatgtcg ctatttttat tctgaccttc ggctgtgagt tcccggcgct gggcaaacat
    22021 gcgccaaaaa cggatgctcc ggtagagaaa gaaaagtggg ggatcggcgt actgtttctc
    22081 tctgttgcgg cgctgtgcta catcctcggt cagttaggtt ttatctcctg ggtgcctgag
    22141 tatgccaaag gcctgggcat gagcctgaac gacgcgggca cgctggtgag taacttctgg
    22201 atgtcataca tggtcggcat gtgggcgttc agctttattc ttcgcttctt tgatttgcaa
    22261 cgcattctga ccgtactggc tggtctggct gcgattctga tgtacgtctt taacaccgga
    22321 acaccagcac atatggcgtg gtcaattctc gctctgggct tcttctccag cgcgatctat
    22381 accaccatca tcaccctggg ttcacagcag accaaagtac cgtcgccaaa actggttaac
    22441 tttgtcctga cctgcggaac catcggtact atgttgacct ttgtggttac cggcccgatt
    22501 gttgaacata gcggtccgca ggcggcactg cttacagcaa acggtctgta cgctgtcgtc
    22561 tttgtgatgt gcttcctgtt aggtttcgtc agccgtcacc gtcagcataa caccctgacc
    22621 tctcattaat tgctaatgcc ggacggcact atcgtcgtcc ggccttttcc tctcttcccc
    22681 cgctacgtgc atctatttct ataaacccgt tcattttgtc tattttttgc acaaacatga
    22741 aatatcagac aattccgtga cttaagaaaa tttatacaaa tcagcaatat accccttaag
    22801 gagtatataa aggtgaattt gatttacatc aataagcggg gttgctgaat cgttaaggta
    22861 ggcggtaata gaaaagaaat cgaggcaaaa atgagcaaag tcagactcgc aattatcggt
    22921 aacggtatgg tcggccatcg ctttatcgaa gatcttcttg ataaatctga tgcggccaac
    22981 tttgatatta ccgttttctg tgaagaaccg cgcatcgctt atgaccgcgt acacctctcg
    23041 tcttacttct ctcaccacac cgccgaagag ctgtcgctgg tgcgcgaagg cttctacgag
    23101 aaacacggca tcaaagttct ggtcggcgaa cgcgctatca ccatcaaccg tcaggagaag
    23161 gtgattcact ccagcgccgg gcgtaccgtt ttttatgaca agctgatcat ggcgaccggt
    23221 tcctacccgt ggatcccgcc aatcaaaggt tctgatactc aggactgctt tgtctatcgc
    23281 accattgaag acctcaacgc cattgaatcc tgcgcccgtc gcagcaaacg cggtgccgtt
    23341 gttggtggcg gcctgttagg tctggaagcc gctggcgcgc tgaaaaactt aggtattgaa
    23401 acccacgtta tcgaatttgc ccctatgctg atggcggaac agcttgatca gatgggtggc
    23461 gagcagctgc gtcgcaaaat cgaaagtatg ggcgtgcgcg ttcacaccag caaaaacacc
    23521 cttgagattg tgcaggaagg tgtcgaagcg cgtaaaacca tgcgttttgc cgacggcagc
    23581 gagctggaag ttgactttat cgtcttctct accggtatcc gtccgcgcga taagctggca
    23641 acccagtgtg gtctggacgt tgctccgcgt gggggtatcg tcattaatga ttcctgccag
    23701 acttccgatc cggatatcta cgccatcggt gaatgcgcaa gctggaacaa ccgtgtattt
    23761 ggtctggtag caccaggcta caaaatggcg caggtcgccg ttgaccatat tctgggtagc
    23821 gaaaacgcct ttgaaggtgc tgaccttagc gccaagctga aactgctggg cgtagacgta
    23881 ggcggtattg gtgatgcgca cggtcgcacg cctggcgcac gtagctacgt ttacctcgac
    23941 gaaagtaaag agatctacaa acgcctgatt gtcagcgaag acaacaaaac cctgctcggt
    24001 gcggtactgg tgggcgatac cagcgactac ggtaacctgc tgcaactggt gctgaacgct
    24061 atcgaactgc cggaaaaccc ggattccctg atcctgccag cacactctgg tagcggcaag
    24121 ccgtctatcg gtgttgataa actgccggac agcgcgcaaa tctgctcctg cttcgacgtc
    24181 accaaaggtg atctgattgc tgccatcaac aaaggctgcc acaccgttgc ggcgctgaaa
    24241 gctgaaacca aagcgggtac tggctgcggt ggctgtatcc cgctggttac tcaggtactg
    24301 aacgcggaac tggcgaaaca gggcatcgaa gttaacaaca acctgtgcga acactttgct
    24361 tattcgcgtc aggaactgtt ccatttgatc cgcgttgaag gcattaaaac cttcgaagaa
    24421 ctgctggcga aacacggcaa aggctacggt tgtgaagttt gtaaaccaac cgtcggttcg
    24481 ctgctggcct cctgctggaa cgaatacatt ctgaagccgg aacatactcc gctgcaggat
    24541 tctaacgaca acttcctcgc taacatccag aaagacggaa cctactcggt gatcccgcgc
    24601 tctccgggcg gtgaaatcac cccggaagga ctgatggcag tgggtcgtat cgcgcgtgaa
    24661 tttaatctct acaccaaaat caccggctct cagcgcctgg cgatgtttgg cgcacagaaa
    24721 gacgatctgc cggagatctg gcgtcagctg attgaagctg gcttcgaaac cggtcatgcc
    24781 tatgcgaaag cactacgtat ggcgaaaacc tgcgtgggta gcacctggtg ccgctacggc
    24841 gttggcgaca gcgtcggcct cggcgtggaa ctggaaaacc gctacaaagg catccgtacg
    24901 ccgcacaaaa tgaagttcgg tgtctccggc tgtacccgtg aatgttcaga agctcagggt
    24961 aaagacgtgg gtattatcgc cactgaaaaa ggctggaacc tgtatgtttg cggtaacggc
    25021 ggcatgaaac cgcgtcatgc ggatctgctg gcagcggata tcgatcgcga aacgctgatc
    25081 aaatatctcg accgcttcat gatgttctac atccgtaccg ccgacaaact gacgcgtacc
    25141 gcgccgtggt tagaaaacct cgaaggcggc atcgattatc tgaaagcggt gatcattgac
    25201 gacaaactgg ggctgaacgc acatctggaa gaagagatgg cgcgcctgcg tgaagcggta
    25261 gtgtgtgagt ggactgaaac ggtcaataca ccgtccgcgc agactcgctt caaacacttc
    25321 atcaacagcg acaagcgtga cccgaacgta cagatggtgc cagagcgcga acagcaccgt
    25381 ccggcaacgc cgtatgaacg tatcccggta actctggtgg aggacaacgc atgagccagt
    25441 ggaaagacat ctgcaaaatc gatgacatcc tgcctgaaac cggcgtctgc gcgctgttag
    25501 gtgacgagca ggtagcaatt ttccgcccgt atcacagcga tcaggtgttt gcgatcagca
    25561 acatcgaccc gttcttcgag tccagcgtgc tgtcacgcgg gctgattgcg gaacaccagg
    25621 gcgagctgtg ggtcgccagc ccgctgaaaa aacagcgttt tcgcttaagt gatggcttgt
    25681 gcatggaaga cgaacagttt tccgtcaaac attacgaagc acgagtgaaa gacggcgtgg
    25741 tgcagctgcg cggttaatgt tttaacggga ggcgcaatgc ctcccctttt tgcatggtcc
    25801 tgtaatgacc ttcggtatat tgcaggacat tttttaaact ttttgtttta tttttttgtt
    25861 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaacg
    25921 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25981 tggcgggcgc gtatgtgggt cttgggatca tcttgatttt cacgctcggt aatttgctcg
    26041 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26101 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26161 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26221 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26281 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26341 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26401 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26461 ctggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26521 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26581 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26641 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacggc tgccggttaa ttactaaggg
    26701 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26761 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26821 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26881 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26941 cagcgacgga tgatgacgcg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    27001 tcttctgtaa cgtggtcgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27061 accgctcacc gctcatggtc gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27121 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27181 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27241 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27301 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27361 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27421 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27481 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27541 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27601 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27661 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27721 cctattcggg tattccgctc acgcatcgcg attatgccca gagcgtgcgc ttaattaccg
    27781 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27841 tggtgttcta tatggggctg aatcaggctg cgactattca gcaaaagctg attgaacacg
    27901 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacagcagtc acgcagcgcg
    27961 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    28021 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaaccatt
    28081 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28141 ataaaacgta gccccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28201 acttacaaaa ttaagtcatt cgctcactga cgccaattaa atatt
//
LOCUS       DAPHDW010000050        28095 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.050, whole genome shotgun sequence.
ACCESSION   DAPHDW010000050 DAPHDW010000000
VERSION     DAPHDW010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 28095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28095
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.050"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            20..709
                     /locus_tag="RH057_002149"
     CDS             20..709
                     /locus_tag="RH057_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001382949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV3128512.1"
                     /translation="MTNDFQHEARILNSIWLITALGLVTTAGLAWVAKYLEITATKYD
                     SPQMYVAIGLLLLCMYGLSKDINKINAVIAGVIYLFLLSLVAIVVASLAPVPAIIVVF
                     STAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIMNAALMSERPVWVISCLM
                     IVLWSGIISHGRNKLLELAGKCHSEELWSPVRCAVTGALTLYYYFIGFFGILAAIAIT
                     LVWQRHTRFFH"
     gene            complement(745..1701)
                     /locus_tag="RH057_002150"
     CDS             complement(745..1701)
                     /locus_tag="RH057_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HDV3128513.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRIFSLSITTNWLGYILLAGIIFTAGVVELPDHWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(1701..2942)
                     /gene="clsB"
                     /locus_tag="RH057_002151"
     CDS             complement(1701..2942)
                     /gene="clsB"
                     /locus_tag="RH057_002151"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HDV3128514.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DDVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     ARREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLAQVDP
                     PAQPTMETQDRVETENTGVKP"
     gene            complement(2939..3700)
                     /locus_tag="RH057_002152"
     CDS             complement(2939..3700)
                     /locus_tag="RH057_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV3128515.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            3833..4243
                     /locus_tag="RH057_002153"
     CDS             3833..4243
                     /locus_tag="RH057_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HDV3128516.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(4205..5311)
                     /locus_tag="RH057_002154"
     CDS             complement(4205..5311)
                     /locus_tag="RH057_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3128517.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(5322..6455)
                     /locus_tag="RH057_002155"
     CDS             complement(5322..6455)
                     /locus_tag="RH057_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3128518.1"
                     /translation="MSNPILSWRRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQAGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(6448..8184)
                     /locus_tag="RH057_002156"
     CDS             complement(6448..8184)
                     /locus_tag="RH057_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3128519.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGNATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPITRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(8177..9172)
                     /gene="hlyD"
                     /locus_tag="RH057_002157"
     CDS             complement(8177..9172)
                     /gene="hlyD"
                     /locus_tag="RH057_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HDV3128520.1"
                     /translation="MKKPVVIGLAVVALAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGNEAGHE"
     gene            complement(9175..9846)
                     /gene="cecR"
                     /locus_tag="RH057_002158"
     CDS             complement(9175..9846)
                     /gene="cecR"
                     /locus_tag="RH057_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HDV3128521.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            10075..11439
                     /gene="rhlE"
                     /locus_tag="RH057_002159"
     CDS             10075..11439
                     /gene="rhlE"
                     /locus_tag="RH057_002159"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
                     /protein_id="HDV3128522.1"
                     /translation="MSFDSLGLSPDILRAVAEQGYREPTPIQQQAIPAVLEGRDLMAS
                     AQTGTGKTAGFTLPLLQHLITRQPHAKGRRPVRALILTPTRELAAQIGENVRDYSKYL
                     NIRSLVVFGGVSINPQMMKLRGGVDVLVATPGRLLDLEHQNAVKLDQVEILVLDEADR
                     MLDMGFIHDIRRVLTKLPAKRQNLLFSATFSDDIKALAEKLLHNPLEIEVARRNTASD
                     QVTQHVHFVDKKRKRELLSHMIGKGNWQQVLVFTRTKHGANHLAEQLNKDGIRSAAIH
                     GNKSQGARTRALADFKSGDIRVLVATDIAARGLDIEELPHVVNYELPNVPEDYVHRIG
                     RTGRAAATGEALSLVCVDEHKLLRDIEKLLKKEIPRIAIPGYEPDPSIKAEPIQNGRQ
                     QRGGGGRGQGGGRGQQQPRRGEGGAKSASAKPAEKPSRRLGDAKPAGEQQRRRRPRKP
                     AAAQ"
     gene            complement(11671..12153)
                     /gene="ybiA"
                     /locus_tag="RH057_002160"
     CDS             complement(11671..12153)
                     /gene="ybiA"
                     /locus_tag="RH057_002160"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HDV3128523.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAAWPIKVDGKTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRDRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRALLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            12273..14423
                     /gene="dinG"
                     /locus_tag="RH057_002161"
     CDS             12273..14423
                     /gene="dinG"
                     /locus_tag="RH057_002161"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HDV3128524.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVARREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITAPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDEVLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEAQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKHLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGEHSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            14451..15413
                     /gene="ybiB"
                     /locus_tag="RH057_002162"
     CDS             14451..15413
                     /gene="ybiB"
                     /locus_tag="RH057_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HDV3128525.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALRLSSVSHPEYIGRVAKFFSDI
                     GGRALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDTAGSELLPQAKDPETTAQ
                     WIERCLAGSEPIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            15554..16639
                     /gene="hcxB"
                     /locus_tag="RH057_002163"
     CDS             15554..16639
                     /gene="hcxB"
                     /locus_tag="RH057_002163"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HDV3128526.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKTVKESGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCAAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGALLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQKQGIPLDAGS
                     WQAICDAARQIGMPEETLQAFCQQLAS"
     gene            complement(16868..17128)
                     /locus_tag="RH057_002164"
     CDS             complement(16868..17128)
                     /locus_tag="RH057_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3128527.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(17393..17659)
                     /gene="ybiI"
                     /locus_tag="RH057_002165"
     CDS             complement(17393..17659)
                     /gene="ybiI"
                     /locus_tag="RH057_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HDV3128528.1"
                     /translation="MASGWANDDAVNEQINSTIEDAIARARGEIPRGESLDECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDSQLR"
     gene            complement(17733..18410)
                     /gene="ybiX"
                     /locus_tag="RH057_002166"
     CDS             complement(17733..18410)
                     /gene="ybiX"
                     /locus_tag="RH057_002166"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HDV3128529.1"
                     /translation="MMYHIPGVLSPQNVARFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSTLYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDNNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(18452..20734)
                     /locus_tag="RH057_002167"
     CDS             complement(18452..20734)
                     /locus_tag="RH057_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
                     /protein_id="HDV3128530.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQTNADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGVG
                     AFFAGENGNSTTGDAIYMRGADTSNSIYIDGIRDIGSVSRDTFNTEQVEVIKGPSGTD
                     YGRSAPTGSINMISKQPRNDSGIDASASIGSAWFRRGTLDVNQVIGDTTAVRLNVMGE
                     KTHDAGRDKVKNERYGVAPSIAFGLGTANRLYLNYLHVTQHNTPDGGIPTIGLPGYSA
                     PSAGTAALNHSGKVDTHNFYGTDSDYDDSTTDTATMRFEHDINDNTTIRNTTRWSRVK
                     QDYLMTAIMGGASNITQPTSDVNSWTWSRTANTKDVSNKILTNQTNLTSTFYTGSIGH
                     DVSTGVEFTRETQTNYGVNPVTLPAVNIYHPDSSIHPGGLTRNGANANGQTDTFAIYA
                     FDTLQITRDFELNGGIRLDNYHTEYDSATACGGSGRGAITCPAGVAKGSPVTTVDTAK
                     SGNLVNWKAGALYHLTENGNVYINYAVSQQPPGGNNFALAQSGSGNSANRTDFKPQKA
                     NTSEIGTKWQVLDKRLLLTAALFRTDIENEVEQNDDGTYSQYGKKRVEGYEISVAGNI
                     TPAWQVIGGYTQQKATIKNGKDVAQDGSSSLPYTPEHAFTLWSQYQATDDISVGAGAR
                     YIGSMHKGSDGAVGTPAFTEGYWVADAKLGYRVNRNLDFQLNVYNLFDTDYVASINKS
                     GYRYHPGEPRTFLLTANMHF"
     gene            complement(20999..21259)
                     /locus_tag="RH057_002168"
     CDS             complement(20999..21259)
                     /locus_tag="RH057_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3128531.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMFGTATIYK"
     gene            21535..22461
                     /gene="rlmF"
                     /locus_tag="RH057_002169"
     CDS             21535..22461
                     /gene="rlmF"
                     /locus_tag="RH057_002169"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HDV3128532.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVAFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            complement(22458..24683)
                     /gene="ybiO"
                     /locus_tag="RH057_002170"
     CDS             complement(22458..24683)
                     /gene="ybiO"
                     /locus_tag="RH057_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
                     /protein_id="HDV3128533.1"
                     /translation="MRWILFILFCLLGAPAHAVSIPGVTTTTTTDSTTEPAPEPDIEQ
                     KKAAYGALADVLDNDTSRKELIDQLRTVAATPPAEPVPKIVPPTLVEEQTVLQKVTEV
                     SRHYGEALSARFGQLYRNITGSPHKPFNPQTFSNALTHFSMLAVLVFGFYWLIRLCAL
                     PLYRKMGQWARQKNRERSNWLQLPAMIIGAFIIDLLLLALTLFVGQVLSDNLNAGSRT
                     IAFQQSLFLNAFALIEFFKAVLRLIFCPNVAELRPFTIQDESARYWSRRLSWLSSLIG
                     YGLIVAVPIISNQVNVQIGALANVIIMLCMTVWALYLIFRNKKEITQHLLNFAEHSLA
                     FFSLFIRAFALVWHWLASAYFIVLFFFSLFDPGNSLKFMMGATVRSLAIIGIAAFVSG
                     MFSRWLAKTITLSPHTQRNYPELQKRLNGWLSAALKTARILTVCVAVMLLLSAWGLFD
                     FWNWLQNGAGQKTVDILIRIALILFFSAVGWTVLASLIENRLASDIHGRPLPSARTRT
                     LLTLFRNALAVIISTITIMIVLSEIGVNIAPLLAGAGALGLAISFGSQTLVKDIITGV
                     FIQFENGMNTGDLVTIGPLTGTVERMSIRSVGVRQDTGAYHIIPWSSITTFANFVRGI
                     GSVVANYDVDRHEDADKANQALKDAVAELMENEEIRGLVIGEPNFAGIVGLSNTAFTL
                     RVSFTTLPLKQWTVRFALDSQVKKHFDLAGVRAPVQTYQVLPAPGAAPAEPLPPGEPT
                     L"
     gene            complement(24800..25522)
                     /gene="glnQ"
                     /locus_tag="RH057_002171"
     CDS             complement(24800..25522)
                     /gene="glnQ"
                     /locus_tag="RH057_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HDV3128534.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALENVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     DPQVLIKNPPSQRLQEFLQHVS"
     gene            complement(25519..26178)
                     /gene="glnP"
                     /locus_tag="RH057_002172"
     CDS             complement(25519..26178)
                     /gene="glnP"
                     /locus_tag="RH057_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HDV3128535.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            complement(26317..27063)
                     /gene="glnH"
                     /locus_tag="RH057_002173"
     CDS             complement(26317..27063)
                     /gene="glnH"
                     /locus_tag="RH057_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HDV3128536.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFGTEPK"
     gene            complement(27467..27970)
                     /gene="dps"
                     /locus_tag="RH057_002174"
                     /gene_synonym="pexB"
     CDS             complement(27467..27970)
                     /gene="dps"
                     /locus_tag="RH057_002174"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HDV3128537.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
BASE COUNT         6644 a         7310 c         7488 g         6653 t
ORIGIN      
        1 ttcgcgctca gcaaataaca taactaatga ttttcagcac gaagccagga tattaaacag
       61 catatggctc attaccgctt taggtttggt gacaaccgca ggactggcct gggtagcgaa
      121 gtatctcgaa attacggcaa caaaatatga ttcaccgcaa atgtatgtcg ccataggatt
      181 attattactt tgtatgtatg gcttaagtaa ggatatcaac aagataaatg ccgtcatcgc
      241 aggcgtaata tatctatttt tactctcttt ggtggcgatt gtcgttgcta gtttagctcc
      301 tgtacctgcc attatcgtcg tgttcagcac tgcgggcgcg atgtttttaa tcagtatgct
      361 ggccggttta ttatttaatg ttgatcctgg ttctcaccgt tttatcatta tgatgacgtt
      421 gacagggttg gccctggtga tcatcatgaa tgccgcatta atgagtgaac gacccgtttg
      481 ggtaataagt tgcttaatga ttgtgttatg gtcaggcatt atctcgcatg gacgaaataa
      541 gctccttgaa ttggcgggga aatgccatag tgaagagttg tggagtccgg ttcgttgcgc
      601 tgttacaggt gcattaacac tctattacta ttttatcggc ttctttggga tacttgccgc
      661 gatagctata acgcttgtct ggcaaaggca tacgcgtttt tttcattagt cgtaaatgca
      721 gatactaaag aacttaacta aacttcacat cgccgcttca tttttcgccc gtagcttctt
      781 cgcctggctt tccagtagca ggtaacagat cagcgccagc agcagcggga taaagtaata
      841 caggacgcga taagcgagca gggcggcgat aatgctccct ttcgaggtat gctccccagc
      901 cagtagtgcg ataaacaccg cttccagcac accgatcccc gccggaatat gcacgatgac
      961 gccagcaata ctactaacca gtaacacgcc cagtacaaag aaatagttca cgctttgacc
     1021 aagtaacagc cagataatcg cccccattac catccagtta acgctggaaa tcagcatctg
     1081 agcgagggcg aatttccatg aaggcagcac cagcttttgc cctttgatgg tcatatggcg
     1141 gtgcttcgcg aaagcgcaaa accacaaata aaccgcgata atcatcagta agccaatgcc
     1201 gagaatacgc agcgtagttt gatcgacata ccagtgatcc ggcaactcca ccacgcctgc
     1261 ggtaaagata atccctgcca gtaaaatata gcccagccag ttggtggtaa tactgagcga
     1321 gaaaatccgc gtaatagtgc tgcccggtaa ccctagtcgg gagtacaaac gatagcgcat
     1381 accaatgccg ccgacccagg tactgagcgt caggttgaag gcgtagcaga taaacgacac
     1441 cagcatcacc tggcgcttcg ccagtttgtg accgcagtaa aagcgggcga gcaggtcata
     1501 gcagccgtaa atcagatagc tgacgaccac cagcccgacc gcactaagca gcgcaacgcg
     1561 attgtagtcg cggatgacct tccagacctc ttcccagtcc acttttttgg cgtagaccac
     1621 cagcaacaca atcaccgcga taaaaaacag ccaggtgagg atcttctttg ctaagcgcca
     1681 gcgcgggtgt gatttactca tcagggtttt acccccgtgt tttcagtttc tacccgatcc
     1741 tgcgtttcca ttgtcggttg tgcgggcgga tcaacctgcg ccagacgtgg tgtgtgtgcc
     1801 ggaagccagc caaccagcgc cgggaagtgg cgtaaaaagt ggaacgccag cacgcttttg
     1861 gtcaggttcc accaggtgcg tttcggcagc atggtctcat cgacctgctg acaatctgcg
     1921 gcaataatgc cgttcagatt atcgcgcagc gtctggttaa aatgacgatc gtggatgatg
     1981 acatttgctt cgagattcag tgacaaactg agcggatcga gattactgga ccctactgtc
     2041 gcccagtgat cgtccatcaa tgccactttg ccgtggagcg ggcggcggcg gtactcaaat
     2101 acctgaacgc cgcctttaac cagatagtta tacagcaagc gcgcaccgac tctgacaatc
     2161 ggcatatccg gttcgccctg aatgatcagt ttgatccgca ccccgcgccg tgccgcttta
     2221 cgcaaggcgt gtaaaaatcg atagccgggg aagaagtagg cgttggcgat aatgacttct
     2281 cgccgcgcct gagtgagcat tttcagatag tgacgttcaa tatcatcgcg atgttcttcg
     2341 ttatcgcgcc agaccagcaa tacctgcgct tctcccggct ggcggttctc ttccgctttg
     2401 tgatgacgtc gccaccagcg tcgtgccgcg ctctgtccag gcaggttttc cagctcaaac
     2461 tggagaatat cttcgacaat cggcccttca aggcgtaccg cgtaatcctg tttagcctct
     2521 ggaccgtagc tggacatatg ctcggcggag taattcagcc cgccaataaa ggcgatacgc
     2581 gcgtcgatca ccacaatttt gcgatgcatc cggcgaaaca cattggtgcg cataccaaaa
     2641 aggcgagggc ggggatcgta gtagcgaaat accacgccag ctgccgtcag ttcattgaca
     2701 aactcatcgc tgagatccgg cgaaccgtag ccatccagca agacttccgc tttaaccccg
     2761 cgttgcgctg ctgccagtag tgccgcatgc agctgtttgc cgacgtcatc ctcaaaccag
     2821 ataaacgttt caagaatgat gcgttcttgt gcctcgccaa tcgccttaaa caccgcggga
     2881 taatattgct cgccgttttc cagcaactgg atcttattgc cttcgcgcca gctacatttc
     2941 ataaatgaat ctccgcactt aaaggggcat gatcagaaag gtgtcgccat gtccgcagcg
     3001 gcaacgcggt tggcgcgctg gcgctggcat ttttgacgta gatcctgtcc agtcgtagta
     3061 gaggaaattg caccggaaac gtgcgcgccg ggcgtccgtg ggcgcgggta aaaatctcat
     3121 ccagtccggc ctgcactttt aacggatgat tagctttttg ccgccagtca ttgaaatcac
     3181 ccgccaccaa taccggttcg ccgtccggta gctcattcac ccattcggcg agcatcgcaa
     3241 gctgcgcctg acggtgcgcc tcacgcaggc ccagatgtac gcacatcaca tgaatcgctt
     3301 ttccggtcat cggtggcaca atgcggcagt agagcacgcc gcgcttttcc gcaccatcga
     3361 ccgaaacatc gcgattctca taatgttcaa tgggataacg cgacagtacg gcgttgccgt
     3421 gatgcccttc cgggtatacg gcattgcgac cgtaggcaaa atcgctccac atcgtgtcgg
     3481 cgagaaactc gtagtgcgag gtatcgggcc agttttccac atgcagcgga tgaacttcgt
     3541 gcgcgcccat cacttcctgc aggcaaacaa tatcggcgct gacggtacgc accgcgtcgc
     3601 gaagttccgg caaaatgaag cgtcggttaa acgcggtaaa acctttgtga atgttgatgg
     3661 tgagcacctt gaacgaaaat tgttgtgttt gatcgggcat aattttcctg tctttgcctc
     3721 ttatctcatt gaaatagtgt agtcggcgtc acaaaaaggt gcggtcttac ggaattttcc
     3781 gtaaagttcg gtactctgag taagtagaga taaattcttc aggagagaag ccatgaagtg
     3841 gcaacaacgt gttcgtgtcg caactggtct aagttgctgg cagattatgt tgcatttact
     3901 ggtagtggcg ctgctggtgg tgggctggat gagtaagact ctggttcacg tcggcgtggg
     3961 attatgcgca ctgtattgtg tcacggtagt gatgatgttg gtgtttcagc gccaccccga
     4021 gcaacgctgg cgtgaggtgg cagacgtgct ggaagagctg accacgacct ggtattttgg
     4081 cgcagcgctg attgtgctgt ggctgttgtc ccgtgtactg gaaaacaact ttttgctggc
     4141 aattgcaggg ctggcaatcc ttgccggccc ggcggtagtg tctttgctgg cgaaagataa
     4201 gaagttacat caccttacgt ctaaacatcg cgtacgccgc tgaccctgtc gtggccgtta
     4261 tcaccagtag cggccacaaa ctattccaca caatatccag actcgcatcc ttcaaataaa
     4321 tctgcttggt aatgtccgta aagtggcgaa tagggttaat ccacgtcagg ttttgcagcc
     4381 atactggcat gttttccacc ggagaaacgt aaccggaaag gagaatggcg ggcatcataa
     4441 aaacaaacac gccgataaac gcctgctgtt gtgtcgagca gagtgatgaa atcaacagac
     4501 cgaatcccac cagcgataaa ccgtaaatca ccatcgtaaa gtagaacagc gccagcgatc
     4561 cggcgaaggg gatttgatac gcccagatac caatcgccag cacaatggtg gcctggaagg
     4621 tggcgacaat taacgccggt acggctttgc cgatgaagat ctgccaggtg gtgagcggcg
     4681 aaaccagtag ctgatcgagc gtaccttgtt cacgttcgcg ggcgacggaa agtgaagtga
     4741 cgatcattac gccgatagtg gtgatcatgg cgatcagcga cggcaccaca aaccatttgt
     4801 agtcaagatt cgggttatac cagttgcgta ccaccagctc gctgttgtta ggtttcggtt
     4861 ttccttccag cagctcctgc tgataatttt tgacgatctg ttgcaggtag ttggcggcaa
     4921 tttgcgcact gttggagtta cgcccgtcga ggatcaactg caacggcgcg gtctggaagg
     4981 tatccagttt gcgcgagaag tcagccggga aacgcaccag tagtaacgcc ttttgtgtgt
     5041 cgatggttgg gcggatctcc tgtgggcttt tcagcagcag cacatgagta aaggcgctgg
     5101 cgcgggcaaa acgttgggtc agctccaccg aatgctcgcc gttatcttca tcgtagatgg
     5161 cgatggtggc gttagtcact tccagcgtgg cggcgaacgg gaacagaatc acctgaataa
     5221 gcacaggtaa aatcagaatc gcgcgggttt gcggttcgcg cagcagcgac tgcaactctt
     5281 tgcggattaa cgtccataag cgatgaaaca tgctcttctc cctaatccag ccgacgtttg
     5341 gttttcagcc acgtcaggcc gataaacatc accgccgaag cgatcaaaaa cagcacgttt
     5401 accaccagca ccactggaat attcccggcg aggaacaggc tttgcagggt gctgacgaaa
     5461 taacgagcgg gaataatgta cgtcaccgcg cggatcaccg cgggcatact gtcgatctga
     5521 aaaataaagc cggaaagcat aatcgacggc agaaaagcgg cgttcagggc gacctgagcg
     5581 gcattgaact ggttgcgggt aatcgtggaa atcagcagcc ccatccccag ggtactgagt
     5641 aaaaacaggc tggagataaa aaacagaatc agcagcgacc cgcgatacgg cacgccgaga
     5701 ataaacactg acaccagcat acacagcaac atcgccagca tcccgagaaa gtaataaggg
     5761 atcagcttac acagcagcag ttccgtgcgg gtaatctccg tagagagcag agcctccatg
     5821 gtgccgcgtt cccattctcg cgccaccacc agcgaggtga gaatcgcgcc gatgaccgtc
     5881 atgataatgg tcaccgcacc ggggataatg aagtgctggc taatcgccgc cgggttaaac
     5941 cagtagcggg tttgtacatc aataagcggt tcaaaagtct gcccgttgtc ctccgctcgc
     6001 tgcatttgcc agatctgcca gatcccttcg acatacccct gtacaaagtt agcggtattc
     6061 ggctcactgc cgtcggtgat cacctgaatc ggtgcggtgg cgttggcgcg ctccatctgt
     6121 tccgcaaaat ccaccggaat aaccaccaga ccgcgaattt tccccgcctg cattttggcg
     6181 atcagttcct gacggttatc gctgatggtg gcgtcgatgt agggcgaacc ggtcatggtg
     6241 tgggtgaaat ccagcgccgc ttcgctacgc tgttccagta aaatcccgac ccgcagcttg
     6301 ctggagtcga ggttaatgcc gtaaccaaaa ataaacagca gcaacagcgg gatcactacc
     6361 gcaatcagcc agctactcgg atcgcgaacg atctgccgcg tctctttgac gcacagcgcc
     6421 cgcacgcgac gccaggaaag gatcgggtta ctcattgcta tgctccttat cccagtcgtg
     6481 gatcaactga ataaaggctt gctccatggt gggatcgggt tgctcgtcgt tggccgactg
     6541 cgctttcaaa tcatccggcg tgccgctggc aattaatttc ccacggtaca ccaggccgat
     6601 gcggtcgcaa tattctgctt catccataaa gtgggtggtg accatcaccg tgacgccttt
     6661 ctctaccatg ctgttgatat gtagccagaa ttcgcggcga gtaatcggat caacgccgga
     6721 cgtcggttcg tcgagaaaca gaatgtccgg ttcatgcatc agcgagcagg ccagtgccag
     6781 ccgctgttta aaacctaatg gcagttcatc ggtggcgtgg gaggcgatac ttttcaggcc
     6841 gaacgcctcg ctcatacggg agattttttc gttctgcgcc cgaccgcgta agccatacac
     6901 accagagaaa aagcgtaaat tctgttcgac cgtcaggtta ccgtagagcg aaaacttttg
     6961 cgccatatag ccgagatgct ggcgcgcttt accagaactc tctttcagat ccatccccag
     7021 caccagcgcc tggccggaag tcggcaccag caaaccgcac atcatcttaa aggtggtcga
     7081 tttacccgcg ccgtttggcc ccagcaaacc aaaaatctcc ccacgtttaa cggcaaagtt
     7141 gacgtgatcg gtggcggcaa aatcaccaaa tttcttggtc agttctttcg cttcgatcac
     7201 cgtctcgccg ggtgtgcctt ctaccgtatg taatattgcg cccagcggcg attctgaggt
     7261 tccggcacca cccagcaaat caataaatgc atcttcaaaa cgcggcgtag tttcgttgat
     7321 gttgatttcc ggcatcccgt cggcatggcg aatatcgtct ggtgtggcct cttttttgag
     7381 gatcagacgt accgatttcc cctgaatcat gccgtcgctg acctgtggca gtttcaaggc
     7441 gcgttgcaat agtttgcggt tgccctcgtg tggactggtc atcagaaaac tgcgtccggc
     7501 catggtttgt gtcagggctt ttggttcccc ctgatacagc aactcgcctt cgttcatcag
     7561 taacacgtca cggcactgct cggcttcgtc gagatacgag gtactccaga ggattaacat
     7621 cccttcgcct gccagctcat gcaccatctg ccacagttcg cgccgtgaga tagggtcaac
     7681 gccgacgccg ggttcatcga gcagcaacac tttcggttcg cccaccaggg tacaggccag
     7741 accgagtttt tgtttcatcc caccggagag cttgcccgcc aggcgtccgg taaacggccc
     7801 aagagacgta aactccagca ggcgagcaaa agtttgctta cgtgcctcgc cggtgacgct
     7861 gcgcaaatcc gcgtacagat tgaggttctc catcaccgtg agatcttcat acagaccaaa
     7921 tttctgcggc atatagccga gcacggcgtg cagcgcgccg tcgtttttga ttggatcaaa
     7981 gccaatcacc gtggcattgc cgctgtcggg tttcagtaat cccgccagca tccgcattag
     8041 cgtggtttta cctgcaccat ccggccccac cagccccgtc acataaccgg cgtgaatggt
     8101 acaatcgagc ggcgcgacgg cgggcttgtc catgcccgga aagcgttttt ccaggccgtt
     8161 cagcgtgata acggcatcat tcatgtcctg cctcgttacc gaattgcacc gtcaccggca
     8221 ttccctggcg taacgcatca tcggcgtcgg tcaccacaat acgcaggcga tagacgaggt
     8281 cggtacgcag atccggtgtt tcgacggttt tcggggtaaa ttcagcagtc ggcgaaacga
     8341 aaccaatctg cccgtgatag ggcttgtccg ggcgaccatc ggtataaagc agcactttgc
     8401 gccccggctg ggcctggtca agattccgtt catcaacata ggcacgtacc cacaccggac
     8461 gcgttagtga aacggtaaac accgtgccac cttcattgag aaccgtgcct ggctccaccg
     8521 cgcgcgttaa cagcgtgcca tcagacgggg ctatcaacgt tgagtcctgc aaattcaact
     8581 ccgcctgcgc cagttgcgcc tgcgcctgtt cgaggctggc tttcgcctgg gcgatgtcct
     8641 gttcacggtt accggaacgg tactgacgca atttatcctg tgctgatttc agcgttgcct
     8701 gcgcctggtc gcgcgaggag cgggcatttt ccaggtcgtt tgccgaaatg gtgcggcttt
     8761 tccacaaccc ttgctggcgg ttatagaagt tctgcgcata gtcataggcg gcttgcgcct
     8821 gtttcaccgc tgcggcggcc tgagcgattt cttcatcgcg atacccggca agcatcaggt
     8881 catactgcgc ctgtgccacc gagacgcccg ctttcgcctg catcagggca atctcatatg
     8941 gcttgtgatc cagttcgccc agcacctggc ccgctttgat cgcatcacct tcgtccaccg
     9001 ccagcgattc aacgcgcccc ccaacacgga aactaagatt taccgtacga atatccacgt
     9061 tgccatacag cgtcaggccg ttatcctggc ggctttgata ccaccagtag cctccggcaa
     9121 ccacggcggc aagtgccact accgccaatc cgatcacgac aggttttttc atcactacaa
     9181 actcctttgc gataatcctt gcaaaatcag gtcgatatga caggtcaccg tctggttgat
     9241 cagttcggtc ttttcttcat cgaacgcggt ccagccggta cgtaacagaa ttgtttcttt
     9301 gccaagacgg aacgccagaa tctcaccaat caacgcatga gtatggagga tcatgcgggt
     9361 atcgttggcg tcgcagccgg tccaggcggc aatcaggcgt gtcaggtggc tgtgtagcgg
     9421 gctaatcacc tgttcgtgca ccaggtgata ggctgccgtg ggagagagct gctcacggga
     9481 gataaacttg ctgaggttga cggtgtcatc ctgggtgagc agcttaatca tgttcctgca
     9541 ggcgcgaagg atcagttcac ggatggctgc gcgatcaggc tgtggttgtg cgaacaagcg
     9601 ttcggcctct tcggcgtgcg gacggaactg ctcgccaata aaatcggcaa tccactgggc
     9661 gcaggcgagg tacaaatctt ctttcgaacc gaagtagtag gtgatggcag cgatattctg
     9721 cccggcctgg gcggctatct cgcgggtagt ggcgttcatt ccatattcac caaactgcgc
     9781 cagtgcggca gcaatcagct gttttttcgc ctgttcaccc ttgattgtca tggcaggatt
     9841 attcatcgca cagtccattc ttaatcaaat gattgattaa gattatgact ccatatggga
     9901 gttgtccagt atggctaaga attttagcta cgccagtcac agggataatt tatgcgctgc
     9961 gtcacaaaaa ctgctacact ccgctccctc atgacattgt ggtttttgtc attttccttt
    10021 tcagtatctc cctgaaaact acaccggtaa cggtcggggc ggttcggagt agttatgtct
    10081 ttcgattctt tgggtttaag ccctgatatc ctgcgcgccg ttgccgagca gggttaccgt
    10141 gaacccaccc ctattcagca gcaggcgatc cctgcggtgc tggaaggccg cgacctgatg
    10201 gctagcgccc agaccggtac cggtaaaaca gcgggcttta cgctgccgct gttgcaacac
    10261 ctgatcactc gccagccgca cgccaaaggg cgtcgtccgg tacgtgcgct cattcttacc
    10321 ccgacccgtg aactggctgc gcagattggc gaaaacgtcc gtgattacag caaatatttg
    10381 aacattcgtt cgctggtggt gtttggcggt gtcagcatta acccgcaaat gatgaagctg
    10441 cgcggcggcg ttgatgttct ggtggcaaca ccgggacgtt tgctggacct ggaacatcaa
    10501 aatgcagtga agctggatca ggttgaaatc ctcgtcctcg atgaagctga ccgcatgctc
    10561 gacatgggct ttatccacga tatccgtcgc gtgttaacaa aactgccagc gaagcgccag
    10621 aacctgctgt tctctgcgac cttctctgac gatattaaag ccctggcgga aaaactgttg
    10681 cacaacccgc tggaaatcga agtggcacgc cgcaataccg cgtctgatca ggtgacccag
    10741 cacgttcact ttgtcgataa gaaacgcaaa cgcgaattgc tgtcgcacat gattggtaaa
    10801 gggaactggc agcaggtgct ggtgtttacc cgtaccaaac acggcgctaa ccatctggct
    10861 gagcagctca ataaagatgg catccgtagt gcggcgatcc acgggaataa atcgcaaggt
    10921 gcgcgtaccc gtgcgctggc tgattttaaa tcgggtgaca ttcgcgtact ggtggcgacc
    10981 gatatcgctg cgcgcggtct ggatatcgaa gagttgccgc acgtcgtcaa ctatgaactg
    11041 ccaaacgtac cggaagatta tgtccaccgt atcgggcgta ccggtcgtgc ggctgctacc
    11101 ggtgaagcgt tgtcgctggt gtgtgttgat gaacacaaac tgctgcgtga tatcgaaaaa
    11161 ctgctgaaaa aagagatccc gcgcattgcg attccgggct atgagccgga cccgtcaatc
    11221 aaagccgaac cgatccagaa cggtcgccag caacgtggcg gcggcggtcg tgggcaaggt
    11281 ggtggtcgcg gtcaacagca accacgccgt ggggaaggtg gcgcaaaatc tgcaagcgcg
    11341 aaacctgcag aaaaaccgtc tcgccgcctc ggcgatgcca aaccggcagg cgaacaacaa
    11401 cgtcgccgcc gtccgcgtaa acctgctgct gcgcagtaat ctttaatgcc gggctatgcc
    11461 cggcatcagg ctgatgaaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    11521 gtgaactctg caatatattg aatttgcata cttttgtagg ccggataagg cgtttacgcc
    11581 gcatccggca tttcacaaca agcacttgtc agcaatttga gaacacggga aaaaatttat
    11641 ctgttttacc gcccataagc caccgagacg ttacttctct atagccaatt gttcgcgcaa
    11701 ctccattaaa aggtagccca gtcgattctt gcccttacca ttaccaccgt ctccccagta
    11761 agcatcgttt tccgtatgct caaccagttt tgcgggcgcg gttgccagca agagcgcacg
    11821 cagttctgca tgctgttcga atttagcgcg aagtgctttt cgcatcacct gttctttgac
    11881 cgactcccag tttttacgca gaggcttaga acgatcgcgc cccatgcgtg cggcgaccat
    11941 aggggaagaa acccgacgga tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg
    12001 aaaatagtgt tctgaggtgg gccaggtttt cccgtcaacc ttgatgggcc aggcggcaaa
    12061 gttagaaaaa tcaccgtagt catcgctggt gctgtagaaa tttatgatgg tgtcttgcat
    12121 gacgtgttgg atcctttgtg ctcgaacggg cattaaaccg cattatgttg gtggttattg
    12181 cgagccgctt tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata
    12241 ttggctgttt atacagtatt tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc
    12301 aaattgccgc ctggtataag gcgcttcagg aacagatccc cgactttatt ccccgtgcgc
    12361 cgcagcggca gatgattgcg gacgtcgcca aaacgctggc cggagaagaa gggcggcatc
    12421 tggcgattga agcccccacc ggcgttggga aaacgctctc ctatttgatc cccggcatcg
    12481 ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg
    12541 atcagattta cagcaaagat ttaccgctgc tgaaaaagat cattcccgat cttaaattca
    12601 ctgccgcttt tgggcgtggg cgctacgttt gcccgcgtaa tctgacggcg ctcgccagca
    12661 ctgagcccac gcaacaggat ctgctggcgt ttcttgacga cgaactgacg ccaaataatc
    12721 aggaagagca aaaacgttgt gcgaagctga agggcgatct cgacacttat aaatgggatg
    12781 gtctgcgcga tcatactgat atcgctatag atgacgatct ctggcgtcgt ttaagtaccg
    12841 acaaagccag ctgcctcaac cgcaactgtt actactatcg tgaatgcccg ttttttgttg
    12901 ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg
    12961 cgatggaaag cgaagccgta ctacctgacc cgaaaaatct gctgctggtg ctggacgaag
    13021 gtcatcacct gccagatgtg gcccgggatg cgctggagat gagtgccgaa atcaccgcgc
    13081 cttggtatcg actacagctg gacttgttca cgaaactggt cgctacctgc atggagcagt
    13141 ttcgcccgaa gaccatccca ccgctggcga tccctgaacg tttgaatgcg cattgtgaag
    13201 agttgtatga gcttatcgcc tcgttaaaca acattctcaa tctctacatg cctgccgggc
    13261 aggaggcaga gcaccgtttt gcgatgggcg aactgcctga tgaagtgctg gagatctgcc
    13321 agcggctggc aaaactcacc gagatgctgc gtggtctggc ggagttattt cttaacgatt
    13381 taagtgagaa aaccggcagc cacgatattg tgcgtctgca tcggttgatt ttgcagatga
    13441 accgcgcgtt ggggatgttc gaggcgcaaa gcaaactctg gcggctggcg tctctggcgc
    13501 aatcttccgg tgcgccggtg accaaatggg cgacgcggga agagcgcgaa gggcagctgc
    13561 atctctggtt tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc
    13621 gcagtattcc gcacattatt gtcacctccg caaccttgcg ttcgctgaac agtttttcgc
    13681 gtttgcagga gatgagtggt ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt
    13741 ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt
    13801 ccatcgacaa cgaagagcag catattgccg aaatggcggc ctttttccgt gagcaggtgg
    13861 agagcaaaaa acatctcggt atgttggtac tgtttgccag cggacgggcg atgcagcgct
    13921 ttctcgacta tgtgacggat ttacgcctga tgctgctggt gcagggcgat cagccgcgtt
    13981 atcgcttagt tgaactgcac cgcaaacgcg ttgccaacgg tgagcatagt gtgctggtgg
    14041 gcttacagtc atttgccgaa gggcttgatt tgaaaggtga tctgctcagc caggtgcata
    14101 tccacaaaat cgcttttccg cccatcgaca gcccggtggt gatcaccgaa ggggaatggc
    14161 tgaaaagcct caaccgctat ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc
    14221 tgattcagca ggttgggcga ctgattcgaa gccacggttg ctggggcgaa gtggttatct
    14281 acgacaaacg cttgctgacc aaaaattatg gcaagcgact actggatgca ttaccggtat
    14341 ttccgataga gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat
    14401 ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa atggactatc
    14461 gcaaaatcat taaagagatc gggcgcggga aaaaccacgc gcgcgattta gaccgggata
    14521 ctgcgcgcgg tctgtatgcc catatgctca atggtgaagt ccctgacctt gagttgggcg
    14581 gcgtattgat tgcgctacgt atcaaagggg aaggggaagc agagatgctc ggcttttacg
    14641 aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg atgccgattg
    14701 tcatccccag ttacaacggc gcgcgtaaac aggccaacct gacgccgttg ttggcgattc
    14761 tcctgcacaa actcggtttt cctgtggtgg ttcacggggt tagcgaagat ccaacccgcg
    14821 tgctgactga aaccattttt gaattgatgg gtattacgcc aacgttgcac ggcgggcagg
    14881 cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc agtcggcgcg ttttgcccgc
    14941 cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt gcacataccc
    15001 tggcgaaact ggcgacgcca tttgccgaag gtgaggcgct gcgtctttcc agcgtttcgc
    15061 atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg cgggcgctac
    15121 taatgcatgg tactgaaggt gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc
    15181 tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggacactgct ggtagcgagt
    15241 tactgccaca agcaaaagat ccggaaacca cggcgcagtg gattgagcgt tgccttgctg
    15301 gcagcgaacc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg
    15361 gtgaagcggc aactatcagc gacggcctgg cgcgcgttaa tcaggcattt taattctttt
    15421 ctcagccgga tgagccatgt catccggctt ttaccccacc gcaatatgaa attcctgcat
    15481 ctttattgac cttcccacgc ccggcgtgca gcataaaaaa acaacaaaca cataacataa
    15541 acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc acagttttat
    15601 tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag tggccgatca
    15661 tttaatcgcg gcaaacctgg cagggcatga ttcacatggt attggcatga tcccaagcta
    15721 tgtacgctcc tggagtcagg ggcacctgca aattaaccat catgccaaaa ccgttaaaga
    15781 gtcgggggcg gcagtcacgc tcgatggcga tcgcgcattt ggtcaggtcg cggcacatga
    15841 agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg tggcgctaca
    15901 taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgtgcag cggcggggtt
    15961 tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcaccgt tccacggtcg
    16021 cgacagccgc tttggcacca atccgttctg tgtggttttc cctcgtaaag ataatttccc
    16081 gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg tcgcctggca
    16141 taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc cgacgaccaa
    16201 tccggcggta atgcaggagt cgccgttggg ggcgctgttg acctttgccg aacataaagg
    16261 ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg gtaaaacgac
    16321 gcatcaggaa acgttacaaa ccagtcccga tgccattctt aactgcatga ccactatcat
    16381 catcaacccg gaactcttcg gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga
    16441 gtgggtgaaa gcctcgccgc atgatgatga taagccgatt ttgctaccgg gcgagtggga
    16501 agtgaacacg cgtcgcgaac ggcagaagca ggggattcca ctggatgcgg gaagctggca
    16561 ggccatttgt gatgcagcgc ggcagattgg tatgccggaa gagacgttgc aggctttctg
    16621 tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa caagggtaac
    16681 ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg gaaatacttg
    16741 tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta ggcctgataa
    16801 gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcgtaaacgc cttatccgtc
    16861 ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg gtggcggaag
    16921 tgatgctgta tccactggca ccggctgccg cggctttctc agccagtttc gcttccaggg
    16981 catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg ttctgtgcct
    17041 gggatgccgt taccggttcc gcggcaaaca cgccaaatga cagagttgaa agagccatag
    17101 cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt tttgtataag
    17161 tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata aaatcgaaga
    17221 gaattgaccg ccttgttcaa ataaattgat tgatatctaa catactgata aaccatttat
    17281 ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt taaaggcacg
    17341 gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt agttaacgta
    17401 actggctatc tttcgaacct ctgcgattat atcctgtata cgctggtttt tgtaaatctt
    17461 tctcctgctg acaatgaatg cataagcgca cgccaggaat ggcttcccga cgggcttgcg
    17521 ggatgggggc accgcactct tcacattcat ccaggctttc gccacgcgga atttcacccc
    17581 gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt
    17641 tagcccaacc ggatgccata tcgtcctccc catatcaatg cttgtacagt taagtgtagc
    17701 taatccaggg acgaacttgg gcagttcaag catcagatct ccgaccattc ccgcagcaga
    17761 ttatgataaa gattaagcag cgacaggatc tcttcatttt caccatagcg gcttttcagc
    17821 gactgaatat tgttgtccag ttcaaacagc atggcgcgct ttttatcatc acggatcatc
    17881 gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc
    17941 aggctgctgg agggatacaa cacgagatcg cctgccggga gttttacccg atgttgtccg
    18001 aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc
    18061 gtggcagaaa ggtcagtacg catccagccg ttttgtggat ggctgcgtac tgcgccatcc
    18121 acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt ggaaagggta
    18181 cgcggcaagg ccgcggcaaa gaataaagca tgttggttaa ccgcgttcag cacctcattt
    18241 tgcagggcgg cgtataacgt gctgcgggtg tcgacctgtt gattgttctt aacttgcgca
    18301 ccttgtgcgc cggtggtgac gcgtccatcc acccattcgg cttgttccag ttgttcgcga
    18361 aaccgggcga cgttctgtgg cgataacacg ccgggaatgt ggtacatcat acaagttctc
    18421 caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct gtgagcaaga
    18481 aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag gcgacgtaat
    18541 cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta acccgatacc
    18601 ccagtttggc atcggcgacc cagtaacctt cggtaaacgc tggcgttccc accgcgccgt
    18661 ctgaaccttt atgcatactg ccgatatagc gtgcgcccgc gccaacagag atatcatctg
    18721 ttgcctgata ctggctccat aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg
    18781 aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg gtatagccgc
    18841 caatcacctg ccacgcgggc gtgatattcc cggccacgga tatctcatag ccttcgacac
    18901 gtttcttacc gtattgcgag taagttccgt catcattttg ctcaacttca ttttcgatat
    18961 cagtacggaa tagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc catttggtgc
    19021 caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca ctgttaccgc
    19081 tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa acggcatagt
    19141 taatatagac attaccgttt tccgtcaggt gatacagcgc cccggctttc cagttcacca
    19201 gattgcccga cttggcggtg tcgacggtgg tgaccggaga accttttgcc acaccagctg
    19261 ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggcggt ggcactgtca tattcagtat
    19321 gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat
    19381 cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca
    19441 ggccgccggg atgaatgctg ctgtcaggat gataaatatt taccgcgggt aacgtcaccg
    19501 gattaacgcc gtagttagtc tgcgtttcac gggtaaattc cacgccggta ctgacatcat
    19561 gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg agaattttat
    19621 tactcacatc tttggtattc gccgtgcgtg accaggtcca gctattcaca tcgctggtgg
    19681 gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc tgctttacgc
    19741 gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca
    19801 tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag
    19861 tatcaacttt tccggaatga ttcagagccg ctgttcccgc agatggggca gaataacccg
    19921 gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgggtgaca tgcagataat
    19981 taagatacaa acgattcgct gtaccaaggc caaaagcgat agaaggggcg acgccgtaac
    20041 gctcattttt gactttgtcg cgtccggcat cgtgcgtttt ttcgcccatc acattcaggc
    20101 gcaccgcagt ggtatcacca atgacctgat tgacgtccag cgtgccgcgg cggaaccagg
    20161 cgctgccaat actggcggag gcgtcaatgc cggaatcatt gcgcggctgc ttgctgatca
    20221 tattgatcga gcccgtcggc gcgctgcgcc cgtagtcggt gccggaaggc cctttaatca
    20281 cttcgacctg ctcagtattg aaggtgtcgc gcgagacgct gccgatatcg cgaatgccat
    20341 caatataaat actgttagag gtatcagcac cgcgcatata aatggcgtcg ccagtggtgg
    20401 agttaccgtt ctcacccgca aaaaacgcac ccacgccggg gacgtttttg agcgcgtcgg
    20461 taaggttggt tgcgccctga tctttaatca cttgttcaga aatcaccgtc atcgtgcggg
    20521 tagtatccgc taccggacgc gagaatttcg gatcggcaga ttgttgtggc gcataaagcg
    20581 aaggcgttga tgcttcgaca accagcgtgt catccgcgtt agtttgccct tcggcggcga
    20641 gtgcctgagc cacaggcgtg atgccaatac aaagaccggc aaagaacgtg agcgaatgaa
    20701 attgtctggc agggaaattg cgattgtttt ccattttgca ggtgactttt ttttatatgt
    20761 tgaaattttc actgccacga gaaagcctct ctgcggatgc gatgccactg ttaatctcgt
    20821 gatagtgttc gtgcaagtga taataatttt gataagcatt atcacttggc gcgattttct
    20881 atcacccatt gcgcgaaaaa gaaatacatt tatttacatt gcgttcatat ttgatgtttt
    20941 ttgattacaa aacaggcgaa aactgcatcc agaggcgaat gcagttacgt tggtggggtt
    21001 atttgtagat ggttgcagta ccgaacatct ggtcatttcc gcctgcggaa ttaatgacat
    21061 aacctttcgc gccttgctcg cgcgcttttt ctgccagttt atcctccaga tcgcttaggt
    21121 ttgatgcgcc agttgccgaa acggtaccgg cgggccgtag ctgaccgctg tccaggttac
    21181 tcatcggttg cgcggcataa gccgtgagag agattccgct caggacggtg gcaatcagta
    21241 gtgtgaggca ctttttcata atgacatcct cataaataca acgggtagtg cctgataagt
    21301 gtaggtcgcc agggcgatga aaatgacgca aaaaattgac agagatgtgc ttattttttg
    21361 aacgatatat ttttacaaaa taagacaaat caatgacatg cataatgctg atttttatga
    21421 tgaattgcca taaaccatag ccatgttaag gttttgggga atgataaacg cagccagata
    21481 tttaccctgt cgcgttatgc gggtatctta cgccgctgct taaaggagaa tgctatgtcc
    21541 gcccagaaac cggggttgca tccgcgcaac cgtcatcaca gccgctacga tctcgccacg
    21601 ctttgtcagg tcaatcctga actcaggcaa ttcctcacgc ttacacccgc aggggagcaa
    21661 agcgtagact ttgccaatcc gctggcggtg aaggcgctca ataaggcatt gctggcccat
    21721 ttttacgccg tagcgaactg ggatatcccg gacggttttc tctgcccgcc agtaccgggc
    21781 cgggcggatt atattcatca ccttgccgat ttactggcag aagcaagcgg aacaattcca
    21841 gctaatgcca gcattctgga tatcggcgtt ggtgcgaact gtatttatcc gctgattggc
    21901 gtacatgaat atggctggcg ttttaccggt agtgaaacca gcagtcaggc gttaagtagt
    21961 gcgcaggcga tcatcagtgc taatccgggg cttaaccgcg ccattcgtct gcgtcggcaa
    22021 aaagagagtg gggcgatttt taacggcatc atccataaaa acgagcaata cgacgcgacc
    22081 ttgtgtaacc cgccattcca cgattccgcc gctgcggcac gggcaggtag tgagcgtaaa
    22141 cgccgtaacc tggggctgaa caaagacgat gcactgaact ttggcggcca gcaacaggag
    22201 ttgtggtgtg aaggtggtga agtcgccttt atcaaaaaga tgattgaaga gagcaaaggc
    22261 ttcgcgaagc aggtgatgtg gtttacctcg ctggtttctc gtggtgaaaa cttaccgccg
    22321 ttgtatcgtg ctctgacgga cgtgggcgcg gtgaaggtgg ttaaaaaaga gatggcccag
    22381 gggcaaaagc agagtcgctt tattgcctgg acctttatga acgacgagca gcgccgccgt
    22441 tttgttaatc gccaacgtta aagcgttggc tcccccggcg gcagcggttc agccggggcc
    22501 gcgcccggag caggcagcac ctgataagtc tgcactggcg cgcgaacacc tgccaggtcg
    22561 aaatgctttt tcacctggct gtcgagggca aagcggaccg tccactgttt gagtggcagc
    22621 gtggtgaacg aaacacgcag tgtaaacgcg gtattgctta agccgacaat cccggcaaag
    22681 tttggttcac caataaccag cccgcgaatt tcttcgtttt ccatcaattc cgctaccgca
    22741 tctttcagtg cctgattggc tttatcagca tcttcatggc gatcaacatc gtagtttgcc
    22801 accaccgaac caatgccgcg gacgaagtta gcaaaggtgg ttatcgaaga ccaaggaatg
    22861 atgtgatacg ccccggtatc ctgacgcacg cccacggagc gaatcgacat ccgttccaca
    22921 gtgccggtca acggcccgat agtaaccaaa tctccagtgt tcatgccgtt ttcaaactga
    22981 ataaataccc cggtgataat atctttcacc agcgtttgcg aaccaaacga gatagccaga
    23041 cctaatgccc cagcacctgc cagcaatggc gcgatattga cgccgatttc cgacaacaca
    23101 atcatgatgg tgatggtact gataatcacc gccagcgcgt tacggaacag cgtcagcagg
    23161 gtacgcgtac gggcgctggg tagcgggcgg ccatgaatat ccgaagccag ccggttttcg
    23221 atcaaactgg cgagcactgt ccagccaacc gccgagaaga aaagaatgag tgcgatgcgg
    23281 atcaggatat ctacggtttt ctgccccgcg ccgttttgca gccagttcca gaaatcgaac
    23341 aatccccatg cgctcaacag caacattacc gccacgcaga cggtcagaat acgcgccgtt
    23401 ttcagcgccg ccgaaagcca gccattcagc cgtttttgta gctccggata gttacgctga
    23461 gtatgtggcg agagggtgat ggttttcgcc agccagcggg aaaacatacc ggaaacaaac
    23521 gctgcgatac caataatcgc caggctgcgc accgttgcac ccatcataaa tttcaggctg
    23581 ttgcccggat cgaacaacga aaagaaaaac agcacgataa aataggcgct tgccagccag
    23641 tgccacacca gcgcaaaggc gcggataaac aggctgaaaa aggccagcga atgctccgca
    23701 aagttgagca aatgttgggt aatctctttt ttattgcgaa agatcaggta caacgcccag
    23761 acggtcatgc acagcataat gatgacgttc gccagcgcac ctatctgtac attcacctga
    23821 ttagagataa tcggcacggc cacaatcagg ccataaccta tcaaactgct taaccagctc
    23881 aggcgacgac tccagtaacg ggcgctctcg tcctgaatcg tgaacgggcg cagttcagcc
    23941 acgtttgggc aaaaaatcag gcgtagtacg gctttgaaaa attcaatcag ggcaaaggcg
    24001 ttgagaaaca aactttgttg gaaagcgatg gtgcgactgc ccgcattcag gttgtcgctt
    24061 aatacctggc cgacaaacaa tgtcagtgcc agtaacagta ggtcgataat aaacgccccg
    24121 ataatcatcg ccggaagctg cagccagttg ctgcgttcgc gatttttttg ccgcgcccac
    24181 tggcccattt tgcgatacag cggcagtgcg cacagacgaa tcagccagta aaaaccaaac
    24241 actaataccg ctaacattga aaaatgggtc aacgcattgc tgaaggtttg tggattaaac
    24301 ggcttatgcg gggagccggt gatattgcga taaagttgtc cgaagcgggc ggaaagggct
    24361 tcaccataat ggcggctgac ttcggtgacc ttttgcagca cggtttgctc ttcaaccagc
    24421 gtcggcggca cgatcttcgg gaccggttcc gcagggggcg tcgcggcgac agtacgcaac
    24481 tggtcgatca attctttacg cgaggtgtcg ttatccagca catccgccag tgcgccatag
    24541 gccgcttttt tttgttcgat atccggttcc ggggccggtt cagtcgttga gtccgttgtc
    24601 gttgtggttg taacgccggg tatggatacc gcgtgggcag gtgcgcccag caggcagaag
    24661 aggatgaaca ggatccaccg catgactcct ccagtgagaa aacagagcaa aaagataagt
    24721 atagatgctg ggggaggggc gattttaaat gagaggaatc tggtgtgcct ccctttcggg
    24781 tgaaagggag gcagagaagt taagagacgt gctgcaaaaa ttcctgcaag cgctggctcg
    24841 gcgggttctt gatcaatacc tgcggatcgc catcttccgc aatccggcct ttgtcgataa
    24901 agatcagccg cgaagctact ttctcggcaa aaccgatttc gtgggtcacg atcaccatcg
    24961 tcatcccttc ttcagccaga tcctgcataa ccttcagcac ttcatggcgc agttccgggt
    25021 caagagcgga agtcggttca tcaaacagca tcattttcgg cttcaccgcc agcgcgcggg
    25081 caatcgccac acgctgctgt tgaccaccag aaagttcgga agggtagtga tgtgcacgtt
    25141 ctgccagacc gactttcgcc agcagctcac gcgccagttt ttccgcctct tctttgttcg
    25201 cgccacgcac gcgtagcgga ccaaacatga cgttttccag cgccgtcaga tgcgggaaga
    25261 ggtaaaactg ctggaacacc atacctgctt cctggcgaat caggcgctcg tcaactttcg
    25321 gatcgttaac cttcaggcca tcgacaatca gatcgccgga ggtgatttct tccagtttgt
    25381 tgatgcagcg cagcagggtc gatttaccgg aaccggacgg cccgataatc accacgactt
    25441 cgccctgggc aatgttcaaa tcgatattgt gcagcacctg ggttgggcca aagtgcttgg
    25501 agacgttttt aaattcaatc acaggatttt catccttctt tccagacgac gcagaataaa
    25561 gctcagcacc agggtaataa tcagatagaa caccgccacg gcgctccaga tctcaagggc
    25621 gcggaagtta ccggcaataa tttcttgccc ctgacgggtc agttccgcca cgccgatcac
    25681 aataaacagc gaggtgtctt taatgctgat gatccactgg ttacccagcg gcggcagcat
    25741 acgacgcagt gccagcggta aaatgacgta gcgaatggtt tcccaacgtg aaagaccgag
    25801 cgccagtcct gcttcacgaa aacctttgtg gatagacagc accgcaccac gcgtgatttc
    25861 cgcaatgtac gcgccggagt tgatcatgat ggtgaccacc gccgcagtaa atgggtcgat
    25921 gcgtaagtcg ttaaacgcca tcggcagggc gaaatagata aacatcacct ggacgacgat
    25981 aggtgtgccg cggatcactt caataaagac cagcgcgacg tggttggcta tccaaccacc
    26041 gaaggtgcgt gcaaaacctg ccagcaatcc gattaccaga ccgcctgcca gaccgaggac
    26101 cgaaatccac agggtcattt tggcaccttc aatcagaagc ggaatggcag gccagatggc
    26161 actccagtca aactgcatat gttgttcctg ttaccgtggt gtaaataatc aaattcaggg
    26221 taattaatgg ccccggagcg ggtttgcgcc cctcacccta atcctctccc cagaggggag
    26281 agggaactgc cagtgcgttt tacaggtgta gcgttattat ttcggttcag taccgaacca
    26341 ttttttgtag atttcgttgt aagttccgtt ctcgcgcagg gttttcaacg cgccgttgac
    26401 tttgtcacgc agctcgtcgc tacctttcgg gaacgcaata ccgtattgct gcgcttccag
    26461 agagtcacct accgctttga actgaccgtt accggcggtt ttgatgaagt acagaatgtt
    26521 tggcgtatcg tgcagaacgg cgtctgcgcg gttggtgccc agttccatat aggcgttatc
    26581 gatgttcggg aactgacgca gatctttagt tttgatgttt gctttcgcgt aatcaacgga
    26641 gccagtaccg ctcttcacag caaccacttt cccgtcgaga tctttcacgc ttttcacatc
    26701 gttattgtta gctttcacca tcactaacag gccgcttttg tagtagccgt cagagaaatc
    26761 gatcgctttt ttacgctcgt cggtgatggt aatgcccgcc agcgccagat cgacgttttt
    26821 ggtttgcagt gccggaatga tcccactgaa atccatcggc ttcagttcgt aatccagctt
    26881 cagctcttta gcgatggcag cccacagatc aacgtcaaag cccacatatt tatcgccctg
    26941 tttaaattca aacggaacga aggcggtatc cgtcgcgaca actaattttt tatccgcggc
    27001 atgagaagaa accgcaaaag ccagggtcag tgcagccagt gaaactttta atacagactt
    27061 catagcattt ccttttttct atccacggga cgatcccctg cgtgacattc acatatatga
    27121 aaaaatcgtg ccagttttgc aactccttga tttttcgaga tgcggcaaaa acgtgatgca
    27181 cgatttatag ggcaataccc tgaagatgca ccattctggg gcaccaatct ggtgcgctaa
    27241 aattgtgcac tcaacacagt gattatttag cgtaaaacct gatgaaaaaa caatctttat
    27301 gtaacgattg tgtgatgatg tggatacaaa aaatttactt aatcagctgg agatagcaga
    27361 tggatgcact aaataagtgc gttgaggtgg gctgcaaaac aaaacggcct cctgtcagga
    27421 agccgctttt atcgggtact aaagttctgc accatcagcg atggatttat tcgatgttag
    27481 actcgataaa ccacaggaat ttatccaggt cgcgagacgc ggcggtcagg atatctgcgg
    27541 tgtcgtcatc tttcgcttcg ccaatcgctt tgcgtacgtc attagcgacg attgcgtaac
    27601 ggtcagccag ttctttcagg tgatcctgaa cgttgtggat gtccagcggg taacttttca
    27661 gcggggtttt gctgttgata acttgagtgg tccccagagc tacaccgccc agctgcactg
    27721 cacgttctgc catggtatcc agatgatcga tcagtgcggt gcggaagcca tccagcattt
    27781 catgtacggc aatgaagtta gcgccgcgca tgttccagtg cgcttgtttg gtaatcaaag
    27841 aaagatcaat aaactggata acctggcgat tcagcaactc tactgttgct tttttctcgc
    27901 tgtcggagac atcgttgcgg gtataaagca gattggtcgc ttttgattta actaatttag
    27961 cggtactcat aatttcatat cctcttgatg ttatgtccca gtaattaacg agactaagta
    28021 tagcaccggc tatgtgttcc gctattctgg ctgttcctat cacactaata gtggtaacaa
    28081 gcgtgaaaaa caaaa
//
LOCUS       DAPHDW010000051        27969 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.051, whole genome shotgun sequence.
ACCESSION   DAPHDW010000051 DAPHDW010000000
VERSION     DAPHDW010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27969)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27969)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27969
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.051"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..159
                     /locus_tag="RH057_002175"
     CDS             <1..159
                     /locus_tag="RH057_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001535067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3128538.1"
                     /translation="IFQWLTGIRTENIPPFNPLTDEDNEPVMVFNGERNVPADSLKPQ
                     PLILPDRR"
     gene            complement(268..3783)
                     /locus_tag="RH057_002176"
     CDS             complement(268..3783)
                     /locus_tag="RH057_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV3128539.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSVGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTIHTSFSINFDDGIDKTAETD
                     EEREARYAALQQEWRQYLDDSERLLKNVAGDWIKNPEQYELAEHGYIVKTAQSGGASF
                     HILSLYDHLLVCKKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGTGAMAGRWLPELKSFGAYFPSSTRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPEKDCSSPEKVIELLHGQLAAKSGQLIRLNATWQTLSQ
                     VRAARELIANDIEQYLDNLNKLLSGQEQKVTQLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRSKRQYQIQLFLEDKLGALIAGNQWSEPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIILKEQQAVQEWQRLALDLGFEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQEEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMSLYE
                     HRRCFDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGEQASSGSRYNLLE
                     AETIAAWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQALGKQGISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EIQPPSSPRGLLAKYLFESEKNALSFDYKERKDLKTSGTKIYTLHGVEQHDNFLNQTF
                     ENTDKHITIVSPWLTWQKLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4235..5497)
                     /locus_tag="RH057_002177"
     CDS             complement(4235..5497)
                     /locus_tag="RH057_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDV3128540.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRNEARKLISEGIDPCEQKRAKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLSTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALDLSRLPELLSRISS
                     YKGQPVTQLAVMLNLLVFIRSSELRYARWSEIDIDNAMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQAVAILTELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5695..5770)
                     /locus_tag="RH057_002178"
     tRNA            complement(5695..5770)
                     /locus_tag="RH057_002178"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5735..5737),aa:Phe,seq:gaa)
     gene            complement(5876..6583)
                     /locus_tag="RH057_002179"
     CDS             complement(5876..6583)
                     /locus_tag="RH057_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HDV3128541.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVTEAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSII
                     SIPLLIIQLTLAWAAALILPLTTPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6981..9116
                     /gene="speC"
                     /locus_tag="RH057_002180"
     CDS             6981..9116
                     /gene="speC"
                     /locus_tag="RH057_002180"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3128542.1"
                     /translation="MKSMNIAASSDLVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGATPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LAIIQLGTYDGTVYNARQVIDTVGHLCDYILFDSAWVGYEQFIPMMADSSPLLLELNE
                     NDPGIFVTQSVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSPLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9166..10422)
                     /gene="nupG"
                     /locus_tag="RH057_002181"
     CDS             complement(9166..10422)
                     /gene="nupG"
                     /locus_tag="RH057_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HDV3128543.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10624..11703)
                     /gene="mltC"
                     /locus_tag="RH057_002182"
     CDS             complement(10624..11703)
                     /gene="mltC"
                     /locus_tag="RH057_002182"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HDV3128544.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLQSRNNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQHTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11768..12043)
                     /locus_tag="RH057_002183"
     CDS             complement(11768..12043)
                     /locus_tag="RH057_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HDV3128545.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(12071..13123)
                     /gene="mutY"
                     /locus_tag="RH057_002184"
     CDS             complement(12071..13123)
                     /gene="mutY"
                     /locus_tag="RH057_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HDV3128546.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMARFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAAANNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13284..14003
                     /gene="trmB"
                     /locus_tag="RH057_002185"
     CDS             13284..14003
                     /gene="trmB"
                     /locus_tag="RH057_002185"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HDV3128547.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKGQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAYEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            14003..14329
                     /locus_tag="RH057_002186"
     CDS             14003..14329
                     /locus_tag="RH057_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HDV3128548.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKTVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEERKLDEVRTSEL
                     FDVWWD"
     gene            14513..15232
                     /locus_tag="RH057_002187"
     CDS             14513..15232
                     /locus_tag="RH057_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HDV3128549.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGESSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15408..16454
                     /gene="ansB"
                     /locus_tag="RH057_002188"
     CDS             15408..16454
                     /gene="ansB"
                     /locus_tag="RH057_002188"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HDV3128550.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINTDCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16571..17578
                     /locus_tag="RH057_002189"
     CDS             16571..17578
                     /locus_tag="RH057_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HDV3128551.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAITLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANERWKKDTEQLRRNGVI
                     K"
     gene            complement(17733..18869)
                     /gene="hemW"
                     /locus_tag="RH057_002190"
     CDS             complement(17733..18869)
                     /gene="hemW"
                     /locus_tag="RH057_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HDV3128552.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     NDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPSYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            complement(18862..19455)
                     /locus_tag="RH057_002191"
     CDS             complement(18862..19455)
                     /locus_tag="RH057_002191"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HDV3128553.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDVLGGAPGIYSARYSGEDATDLKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            complement(19463..19753)
                     /locus_tag="RH057_002192"
     CDS             complement(19463..19753)
                     /locus_tag="RH057_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HDV3128554.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            complement(19750..20316)
                     /gene="yggT"
                     /locus_tag="RH057_002193"
     CDS             complement(19750..20316)
                     /gene="yggT"
                     /locus_tag="RH057_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HDV3128555.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQWAHCDFYNPFSQFVVKVT
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFKVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYVINMGVAEVLQATGNMLLPGLWMAL"
     gene            complement(20334..21038)
                     /gene="yggS"
                     /locus_tag="RH057_002194"
     CDS             complement(20334..21038)
                     /gene="yggS"
                     /locus_tag="RH057_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HDV3128556.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNDQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPAPESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            21056..22036
                     /locus_tag="RH057_002195"
     CDS             21056..22036
                     /locus_tag="RH057_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HDV3128557.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGLMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPTVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YIYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQRVGEGRL"
     gene            complement(22212..22628)
                     /gene="ruvX"
                     /locus_tag="RH057_002196"
     CDS             complement(22212..22628)
                     /gene="ruvX"
                     /locus_tag="RH057_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HDV3128558.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(22628..23191)
                     /locus_tag="RH057_002197"
     CDS             complement(22628..23191)
                     /locus_tag="RH057_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HDV3128559.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            complement(23300..24250)
                     /gene="gshB"
                     /locus_tag="RH057_002198"
     CDS             complement(23300..24250)
                     /gene="gshB"
                     /locus_tag="RH057_002198"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HDV3128560.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTRYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            complement(24263..24994)
                     /gene="rsmE"
                     /locus_tag="RH057_002199"
     CDS             complement(24263..24994)
                     /gene="rsmE"
                     /locus_tag="RH057_002199"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HDV3128561.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGKIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            complement(25074..25781)
                     /gene="endA"
                     /locus_tag="RH057_002200"
     CDS             complement(25074..25781)
                     /gene="endA"
                     /locus_tag="RH057_002200"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HDV3128562.1"
                     /translation="MYRYLSIAAVVLSAAFSGPALAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            complement(25876..26373)
                     /locus_tag="RH057_002201"
     CDS             complement(25876..26373)
                     /locus_tag="RH057_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HDV3128563.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAVYRCVHCGE
                     QLVAK"
     gene            complement(26450..27844)
                     /gene="galP"
                     /locus_tag="RH057_002202"
     CDS             complement(26450..27844)
                     /gene="galP"
                     /locus_tag="RH057_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HDV3128564.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGSGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
BASE COUNT         6930 a         7053 c         7131 g         6855 t
ORIGIN      
        1 atttttcagt ggctgaccgg cattcgtact gaaaatatac caccattcaa tccgctgaca
       61 gacgaagata atgaacccgt tatggttttt aacggagaaa ggaatgtacc ggcagacagt
      121 ctgaaaccgc agcctctgat tcttccggac agaagataat catgtcggta tgaatgttat
      181 aattaaggac tgtgcaataa tagccattcc attcagccat cttacgctcg aaaacaatca
      241 ataagatggc tactcaccat tcctgctcta aacctgacgc ctctcaagac tattataaat
      301 agcctcaatc tcacccttca ggttatcacc gcaataaacc attgaggtat cgtatcgttc
      361 atatcgcgct tcccgtgtcg cactaaacca gttgaacgat cccacgcaca gcaaaccatc
      421 atcaccaata acaattttgc tatgaacacg gtttaccagc tttgtagcaa tacccagcgc
      481 attcagtttc tccagcgccg ctttaaagtt ctgctgcttc tcttttcgct tctcaaaatc
      541 attatgttca gtgttgtagc ttctgtcagt gactatcgtg acgttaattc cacgtgaaca
      601 cgccgcaatc atggaatcaa gaaaacccgt ttgctccagc ttttgccagg tcagccatgg
      661 agaaactatc gtgatgtgtt tatcggtatt ttcaaacgtc tgattcagga agttatcatg
      721 ctgctccaca ccatgaagtg tgtagatttt ggtcccggaa gtttttaaat ccttacgctc
      781 tttataatca aaagagagcg cattcttctc ggactcaaag agatattttg ccagcaatcc
      841 tcgcggagat gagggaggct ggatctcaaa cagatccata tcgccgaaga ccaggaagct
      901 atccttcgca cgggagactg caacattcag catgctgtta tcgctatcaa taaacccgcc
      961 gtcttcatgt tttgaataga ctggcgagaa tataacaatc gctctttccg ctccctgaag
     1021 agagtgcacg gtgcccactg tgagcgactt ttcattcgtg cctgtactga taccttgttt
     1081 acccagcgcc tgttttatag tggatacctg cgcgctaaaa ggcgtcacga taccgacaac
     1141 ttcatgaagc gatttgccat aatgcgcttc aatattttgc tggttctctg ccaaccaggc
     1201 cgctatcgtt tcagcctcaa gcaaattata tcgacttcca ctacttgcct gctctccttt
     1261 accatcaata tggagataac ccattgcggg cattaaattg ctctcttcac gccctctttt
     1321 aggcaacaac ttaccgtgat agcagagcgt attacagtat ccaataatat tatcgaagca
     1381 ccggcggtgt tcatataggg acataccacg agccagttcg ggatcatatt gatagcgcga
     1441 agcaaactgc gctattttca taacgctgcc agatgcggca cttttaccaa ggtctgcgat
     1501 tgccgtatat ttctcggtaa tctcttcttg cgtactgcca gacagaattt tttccgccag
     1561 catgttaccg acatcaatcg cgggagcaat actccatatt ggcgggatct gctccgtatc
     1621 gccaatcact aatgccttct tagctaatgc aaacgaggca gcagccactt caggaagcac
     1681 ctgcccggct tcatcgacaa tgagtaaatc ggcaaaatca tacaaataac ttttctcgaa
     1741 tttacgctgt cctttgtgct cacttatctg catattgccg ggcagcatat agcatgtcat
     1801 caccacacat ggagtgagtt tcattcgacg ttgccaacgg gcggttaccc ctttagcgcc
     1861 ttttttcttc ttctcttcct gcagatcatc aatgctggcc atatccatca gccaacgacc
     1921 ttcccagtag tgagtcgtca gtaaaaatgc agggaagcga atctgcgtat cagccagttc
     1981 atcggcctgt gagaagctca gttcctcgtc gccctcaaac cctaagtcaa gagccagtct
     2041 ctgccactcc tgaaccgcct gctgttcttt aagaatgatt tcatgggcgg agtcaatctg
     2101 ctgccggtat gttgtttgct cgcgctcagc ggaattgagc agcccatcaa tattacgttc
     2161 gatagtttca ggctcagacc actgatttcc tgcaatcagc gcgcctaatt tatcttcgag
     2221 aaacaactgt atttggtact gtcgcttact acgaaccgcc gggagccagg aaaataatga
     2281 atagatcagt gattcaccgg cgcgatattt tttccattcc gttttagcac tcttcagttg
     2341 agtgactttt tgttcttgtc cggaaagtaa tttatttaaa ttatcgagat attgctcaat
     2401 gtcgttagca ataagctcac gcgcagcccg tacctggctt aacgtttgcc atgttgcgtt
     2461 cagtcttatc agttgcccgg attttgctgc caactgacca tgcaggagtt caatgacctt
     2521 ttcaggggat gaacaatctt tttcaggaaa ggctgcctta gccttctcca gataaaacag
     2581 cagtgcatcc tctacatact cttttgactc aacctggttg aagaaatctt cagtttgata
     2641 ttttttggct gcctcagctt tacgagtgct tgagggaaaa taagcgccga agcttttcag
     2701 ctcaggtaac catcgtccgg ccattgcacc agtgccctgt gaaaaatctt tcccgaacgc
     2761 ttcgataatg ttcgttacag cctggttgtt cgttgaagtc gcgataataa ccggaggctc
     2821 agatttttcg agagccgctc tggcccactg cgtggcgatg atagaaagca ccagcgtggt
     2881 ttttccggtt cccggaggtc cattaacagc aaggatatcg ccatgtcttg catccagaaa
     2941 atggcttaag gcatcgcgct gagcctttgc cagcggaaac ttatctccgg agtgtccaag
     3001 cctgtcgctg aattttgcgc ctggggccag caaagactct gcagcatgaa cctctcgcga
     3061 ggcgaagcga ttgaagagcg gcacatcctt cttgcaaaca agcaggtgat cataaagaga
     3121 aaggatatgg aaactggcac cgccagattg cgccgtttta acaatataac cgtgctcagc
     3181 gagttcatat tgctcaggat ttttaatcca gtcgccggca acgttcttca gcagcctctc
     3241 tgaatcatcc agatattgac gccactcctg ctgcaaggct gcatatcgtg cttcccgttc
     3301 ttcatccgtt tcggcagtct tatcaatgcc gtcatcaaag ttgatagaga atgaggtatg
     3361 gattgtcttg tatttgtcat actgcccaat ctcaccaatc gaaaatgctc cttttggcaa
     3421 aggttcaagc aggtctctgg gaatggaggt cgctggcgtc ggatataaaa aaccctcacg
     3481 gcttagcaag gctggcgtca ctatcggggt aacaatatca ggcgcgccta cggaacggtc
     3541 cttaccatgc tgcagtaacc ggaaataaac ttttgggcgc aagatgacat cgaccgtttc
     3601 gacatcgtct ttttctccct caaaaaattt actgacaatc gcttcgtcaa gacgtcccgc
     3661 cgctatccca tgccagtgag tgaaattttg ggcgtctttc cgtttaaaac tgccctttcc
     3721 tgactcagca tccgcaagcg agttgcgcca gtatgaggca aaccctaaag cattttcatc
     3781 cattaccagt cctgtgactc aatgaaatcc attgatttat cgaatataaa atcaccgtat
     3841 ggacagctat tgtccatatt gaaaaagttg gatgcaatgt tttgtttata attcgatatc
     3901 aaatgaactg gttgtacgag gttcagaaca caagctcatt aaacggtaag tcaggactat
     3961 tgatttcata acagtgaata cattttttac cgtttataat aactgcctgt gttggacggg
     4021 gataccagag cagccgaaaa caccgggggt tattacctgc tctcatggaa accatatatt
     4081 cctggtgcta atcacagtca taaatagcct gaagaacgtg acgaagggac tttaatggac
     4141 gccggagtaa cttgtcagaa acatcaatct tcgtcatcat ttcaaagaaa aataatcagt
     4201 taaagcagtc cgggctgatt gcccggactg cttactattt taatggattg ttaatctttg
     4261 catattcaaa cggactgata cccttctccc ggttcgcatc cagaaaatcg gcccaccatt
     4321 gcaacatcag tcgccgttct tccagatgtt ctgctttatg gatatacgca gcacgtacac
     4381 cattacgctc ctgatggctc atctgacgtt ccacagcatc gcgggaccac aaacctgatt
     4441 caatcaatgc actgcacgcc atcgcccgga atccatggcc acagacttcc tgggttgtgt
     4501 cataccccat caccctcagg gccttattta cagtattttc actgattggt ttacgcgggt
     4561 catgtgcacc cgtaaatatc agaccatttt caccagccca tgtctgaagt tctgtcagta
     4621 tggctacagc ctgtttgctg agtggcacaa gatgtggtgt tcgcatcttg gagccccggt
     4681 gtgagaattt tacgccgggc agaggttcgc gttcggctgg aatagtccac atggcattgt
     4741 caatatcaat ttcagaccac cgggcgtatc tgagttcact ggaacgaata aaaaccagta
     4801 aattcagcat aacggcaagc tgggtgacag gttgcccctt gtaactgcta atacgcgaca
     4861 aaagttcagg caggcgagaa agatcaagag cggggcgatg ctggcgtttt accgtggtca
     4921 atgcgccagc catatccaga gcaggattat atctgatgat gccagactgt acggcataac
     4981 gcattacagc agatattctt tgctgcagac gactggcgat ttcataaatt tgtttagctt
     5041 ctgcggcacg aacggggata agcaggtccg gggtactgag tgttgtgata tcccggttgc
     5101 cgataaaggg gaaaacgtgt gtctcgaggc ttttgagtac tttatcgctg tgtgattgtg
     5161 cccattgttt attactggca tgccagcgtc gtgcaatatg ttcaaaagag agttgtaaat
     5221 cagggactac ttttttagct cttttttgtt cgcaagggtc aatcccctcc gaaatcagtt
     5281 ttcgggcctc attccgtttt gtacgagctt cggagagaga tacttctgga tacacaccaa
     5341 gtgccagagt cttctcttta cccagaatac gataacggag acgccagtaa cgggaaccat
     5401 taggatgtac cagcaggaac atgccagccc cgtcagtgag tttataagcc ttgtcagtgg
     5461 gctttgcagc ccggattttt gcgtcagtca gtgccatagt tcagccttct gaatgcgtta
     5521 aagagggtat aaattctgtc gaactggggt atacccgcaa ttatacccac acaatggggt
     5581 tgatgtgggg ggaatctggt tgagttcggt agaattgatc tggaggcaga gcgcttaaat
     5641 tgtggcgtcc tgaaacgaaa aacggacctc cgtggaggtc cgttgatatg aatttggtgc
     5701 ccggactcgg aatcgaacca aggacacggg gattttcaat cccctgctct accgactgag
     5761 ctatccgggc aacggggcgc attaaacctg attcgcctcg tctcgtcaat caaatttctt
     5821 caatttactg cagactgcac aatctatcat cactttgccg ctattgcacg cattgtcagg
     5881 caaaccaggc ggtccaggcg gcggaaagcg gcattgccag aaggagtgct ggaagcatgt
     5941 tgaccaccgg gaacatttta atgccacaga tgcgtaatcc ggttgccagc agcaataaac
     6001 cgcctacggc gctgaagtct gccatcatcg acggtgtggt cagcggtaat atcagcgcgg
     6061 cagcccacgc cagcgttaac tggatgatca gtaatgggat actaattatc gacaccgcaa
     6121 tacccagcga gcaggcgaag atcatcgccg taaagaaatc aagaaatgac ttggcgatta
     6181 aaatactcgg atcgccggtc atcccttcgt tcatcgcccc gaagatcccg gtgccgctgg
     6241 cgcaaaacag gacaataatc gcgacataat tctgaataaa agattcatgc gctggcttct
     6301 tacgtgagtg acgaaacaga ttttgtgctt cggtgactgc tgtattgaca cctttctcca
     6361 gcaaacaaat ttcgccaatt agagcgccaa gtagcgtagc taaaaccatc gctggaaggt
     6421 tggcacattt caccaccagt aaaataccaa tccccagcga tgccagacca aaaatagatg
     6481 tcatggagac gcggatacgt tccggtaagc gttggctgag cagtgcgccg agaacgccgc
     6541 ccagtaatac tgcactagcg ttgataaaag ggccgatgac cacaagagtt cctgttaact
     6601 gtattgtttg attttcttat tatgaccgtt tggtgccgtc gatggcttgt ttgttcgcaa
     6661 agtcctggct tgcacgcttt agcgaaaggt gccatgattg cgcgaatttt ctcctctctg
     6721 tacggagttt gcccgatgca cgccacctcc ttacattctc tcgcttatcg ccgtttcgcg
     6781 cgaaacgttt ccctttctat gttactgctc atgcggtgaa gttaacgcac gctcactgca
     6841 ggacaacagt aaaatcagag cgtttctgct tttactgatg tctggcggtc ggagctggtg
     6901 accagtttga cccatatctc atggggcagg gttttccacc ttgtccggta ttcttacttc
     6961 cccgaaacgg gtttgcgctt atgaaatcaa tgaatattgc cgccagtagt gatctggtat
     7021 cccgactttc ttctcatcgt cgcgtggtgg cgttgggaga tactgatttt acggacgtcg
     7081 cggcagtcgt cattaccgct gcggatagtc gcagtggcat tcttgcgttg cttaagcgca
     7141 ccggttttca tctaccggtg tttttgtatt ccgaacatgc tgttgaatta cctgcgggcg
     7201 ttacggcggt aatcaacggc aacgagcagc agtggctgga gctggaatcc gcagcctgtc
     7261 agtatgaaga gaatttgctg ccaccgtttt atgacacgct gacgcagtac gttgagatgg
     7321 gcaacagcac ctttgcttgc cctggacatc aacatggtgc gttttttaaa aagcatcctg
     7381 ccggacgcca tttttacgat ttctttggtg agaacgtctt tcgcgccgat atgtgtaacg
     7441 ctgacgtaaa attgggcgat ctgcttattc atgaaggatc ggcgaaagat gcgcagaaat
     7501 tcgcagccaa agtctttcat gccgataaaa cctattttgt gctgaacggc acatcggcag
     7561 cgaataaagt ggtgacgaat gcgctgttaa cgcgtggcga tctggtgctc ttcgaccgta
     7621 acaaccataa gtcgaatcat cacggcgcgc tgattcaggc gggggcgacg ccggtctatc
     7681 tggaagcttc acgcaacccg tttggtttca ttggcggtat tgatgcgcac tgttttaatg
     7741 aagagtatct gcgccagcaa attcgcgacg ttgcgccaga aaaagccgac ctgccgcgcc
     7801 cgtttcgcct ggcgattatc cagctgggaa cctatgacgg cactgtctac aacgcccgtc
     7861 aggtgatcga taccgttggg catctgtgtg attacattct gtttgattcc gcgtgggtcg
     7921 gttacgaaca atttatcccg atgatggcgg atagctcgcc gctgctgtta gaacttaacg
     7981 aaaacgatcc ggggattttt gtgacccagt cggtgcacaa acagcaggct ggattctcac
     8041 agacgtcgca gatccataaa aaagataacc atatccgcgg acaggcgcgt ttttgcccgc
     8101 ataagcggtt gaataacgcc tttatgctcc atgcttctac cagccctttc tatccgctgt
     8161 ttgctgcact ggatgttaac gccaaaattc atgaagggga gagtgggcgt cggctgtggg
     8221 ctgagtgcgt tgagttgggt attgagtcgc gcaaggctat tcttgcgcgc tgtaagctgt
     8281 tccgcccgtt tatcccgccc gttgttgatg gcaaattgtg gcaggattat ccgacatctg
     8341 tgttagccag cgaccgccgt tttttcagtt ttgagccggg ggcgaagtgg cacggctttg
     8401 aaggatatgc cgcggatcag tattttgttg atccgtgcaa gctgttactc actacaccag
     8461 gtatcaatgc cgaaactggc gaatatagcg actttggcgt tccggcgacg attctggcgc
     8521 actatctgcg tgagaacggc attgtgccgg agaagtgcga tctcaactcc attctgttct
     8581 tattaactcc ggcggaaagc cacgagaagc tggcacaact ggtggcgatg ctggcgcaat
     8641 ttgaacagca tattgaggat gactcgccgc tggctgaggt gttgccgagc gtttataaca
     8701 aatatccggt gcgctatcgc gactacaccc tgcgccagtt gtgtcaggag atgcacgatc
     8761 tgtatgtcag tttcgacgtc aaagacctac aaaaagcgat gttccgccag cagagtttcc
     8821 cgtcagtggt gatgaacccc caggatgcgc atagcgctta tattcgcggt gaagtggagt
     8881 tggtgcggat tcgtgatgcc gaagggcgaa ttgcggcaga aggggcgttg ccttatccac
     8941 ctggcgtgct ttgcgtggta cccggggaag tctggggtgg ggcggttcaa cgttatttcc
     9001 ttgcactgga agaaggggtg aatttgttgc cgggattttc gccggagctg caaggtgttt
     9061 atagcgaaac cgatgcggat ggcatgaaac ggttgtacgg ttatgtgttg aagtaagaat
     9121 aaaaaaaacg ggtcaccttc tggcgacccg tttttctttg cgtaattagt ggctaaccgt
     9181 ctgtgtgcct gtcgggacac gaacgtgttt atatttgaac atcgccatga acgcgaaggc
     9241 cagaaccacg gagtaaccag cgaaaatcaa ccatacggtc tgccagtcgg taatgccgtt
     9301 ttgggtgtac atctcaacaa ctttaccgct cacgatgccg ccgaggatac agccgaagcc
     9361 gttagtcatc atcaggaaca tcccttgtgc actggcgcga attgccgggc taacttcttt
     9421 ttcgacaaac accgaaccag agatgttgaa gaagtcgaat gcgcaaccgt aaacgatcat
     9481 cgacagtacc agcagtacag taccgaacgg agtcgggtcg ccgtaagcaa acagcgcaaa
     9541 acgcaggatc cacgccacaa tactgatcat cattacgttc ttaataccgt agcggcttaa
     9601 gaagaacggg atggtcagaa tgaacagggt ttcagagatc tgcgaaatcg acatgatgat
     9661 tgacgcatgc tgcacaataa agctgctggc aaacatcgga tctttgtcga agctgtgcag
     9721 gaaggtatta ccgaacatgt tggtaatctg cagttccgcg cccagcagca ttgagaagat
     9781 aaagaagatt gccatacgct tgtttttaaa cagcgcgaat gcatcgaggc ccagcagggt
     9841 tgtccagctc tgattcgctt gctgtttagc aaccggaata tgcggcagag tcagggtaaa
     9901 cagaaccaga atggcggaaa gtgctgcgcc aatatacagc tgcatgtggc ttaattcgaa
     9961 gccagacagg ctcaccaccc acattgccat gataaagccg atggtgcccc agatacggat
    10021 tggcgggaag tcagtaacga tatccatccc ggcattttgc aggcgatagt aagagatggt
    10081 gttgattaac ccaagcgttg gcatataagc aaacgagtta atcaatatca caaggaacat
    10141 cgcctccggt gtcgtgacct gtgccgccat gaacagcgtg atagcgccaa tggtgtggca
    10201 aatggcatat acccatttcg cacttaacca tttgtcggcc acaatcccca gcagcgcagg
    10261 cataaagacc gcggcgatac ccagtgagct ataaactgcg ccaatagaag caccgtcaaa
    10321 cttcagggta acaaacatat aggagccgag ggtcgtcagc caacttcccc acagacagaa
    10381 ctgcagaaaa gagaggattt tcagctgcag cttaagattc atgttaattt cctcacatcg
    10441 tgatgcggat gagtgtttga aaaagcacat ttgaccgtgc gagatgatgg gattctatca
    10501 acttcgcgga ctggtttttt gttacctgtg gcaaataatt gcaaacattc ccaatttgca
    10561 aaacgaaatt gtgatgtgga taacattttt gcccctgagc atcgtcaggg gcggttaatg
    10621 gaattatcgg cggcggtagg atttttgcgc ggtattcact ttataaagat aacggcgaga
    10681 ttccgcagag ggatggcggg tcgtcagcgt ctgataaaca tcgcccggcg tcatggtgtt
    10741 aataatattg gcagcctgaa tcttatcatt cgaaaagact cgcagcacgc tgcctgcgcc
    10801 gccgttatag gcggtgatga cggcataacg ccgcgatgtt gggttatcaa ttccgccgag
    10861 ataaacattg ttcagcatcg ccagatacgc ggtgccggta tcaatattgc tggcaggatc
    10921 aaacaagaaa ctgcggctcg gcgtgccgga tttcccctgc gagcggaaca catctttccc
    10981 ggcagtatgt tgtaccacct gcattaatcc cagcgcatcg gaacggctga ccgcatacgg
    11041 gttaaaggaa gattcggtct gcataattgc cagaatcagc gactcatcaa cgccatattt
    11101 ccgtgacgcc tggcggacca tgccgagata tttgtgcgca cgtttatcaa ggtggttcgg
    11161 taccatgtta atggtgacgc tgtagatgat acgcagcccg ttattgcggc tttgcagacg
    11221 gtttttcagc agataatccg cgaagttgct tgcgcgacct tcccagcgaa tcggctgccc
    11281 ggtgttgtcc accacctgac cgtaaaggaa aggttctttc gaaatcgtaa tatcatcaac
    11341 gtcggaatag agatcgaccg aactcggatc gtcacccatc agcaacgttt tgataattgc
    11401 ccggcgcaaa tgcgcggcag gttctgtccc ggcgatagtt tcgatagtaa tcgtaccgtc
    11461 atcgaagttg atgtggctgc gggtctgata ttgatcggtg tatttcacgt agtccttagg
    11521 accggcgatc accacctctt tgaatcccca gatgttctca atattgtggg caaattgccc
    11581 catcagaata tcaaaaccgt tggtatcttt gacccaggct tcgttatagg tatcgccttt
    11641 tttggtcgtc gaacaggaga tgagcaacgg cgcaatcaaa gccagcgcga gatatttttt
    11701 catcattccg ggtgcgtgtt gtgtttgttt ttatgcaagt tgccggaggc gtgctccggc
    11761 actgttttta ttttttatct tccggcgtat agccctcgat atgcacctct ttaccctcga
    11821 acaggaagtt gaccatctcc tgctcaagca gcttgcggtg ctcggcattc atcatgttga
    11881 gtttcttttc attaatcagc atggtttgct tgtgctgcca ctgcgcccag gcttctttgg
    11941 agatctcgtt atagatgcgt tttcccagct cgccggggta cagctgaaaa tcctgacctt
    12001 ctgcttcacg ttgcaggaaa gtacaaaaaa tcgttctgct cataaatcat cctctttatc
    12061 gactcacgcg ctaaaccggc gcgccagtgc gtaactgctg taacaaacgc tccacgggag
    12121 ccgccaggcc aactgacggc ggttgcgcta agttatacca gagcgcattg ccttcatcca
    12181 tgcagccggt gaatgacgac acgggaagcc acataggcac aatatctaag tggaaatggc
    12241 tgaaggtatg gcgaaacgcg gtcagctgcg tcaggttatc ggcagcaatc tgccgttgcg
    12301 ccagccaatg ccgcaaactt tcttcgtcgg caaactgcgg gaaacagtat aaaccgcccc
    12361 acaatccgct cggcggacgc tgcgccagca atacttcatc ttcgtgctgt aatagcaaaa
    12421 agtagccggt gcgctccggc agcgtctgtt tcggtttttt gcccggataa agcgaccagc
    12481 tattgttggc ggcggcaata catccgtttt gtagcggaca gagcgaacat ttcggtttcg
    12541 agcgcgtaca aatcatcgcg cccaaatcca tcatcgcctg attaaaccgt tccacgccga
    12601 ccgcgggcgt cacctgctcg cttaaactcc ataatttatt ctcgacctct tttttcccag
    12661 gccagccgct tacagcatag cagcgcgcca gcacgcgttt gacgttaccg tcgagaatcg
    12721 gaaagtgctt acccagagaa agcgagagaa tcgcgcctgc ggtggaacgc ccgacgcccg
    12781 gcagtgctgc aacttcctca aaggtttccg ggaatttacc gccgtgtaag gtcgccactt
    12841 gttgtgccgc tttatgcaga ttgcgcgcgc gggcgtaata gccaagcccg gtccacaagt
    12901 ggagaacttc gtcgagcggc gcattggcga gatcggtcac cgtcgggaag cgcgccataa
    12961 agcgttcaaa ataggggata acggtcgcaa cctgagtttg ttgcaacatc acttctgaga
    13021 gccatacttt gtagggcgtc ttgtcaattt gccagggcag agtttttcgc ccgtatttat
    13081 cgtaccagtc cagaacctgg gctgaaaatt gcgacgcttg catggtcacc gaattcactg
    13141 ttgttggggg caagattgca gcacagcgac ggcagggtgt aaaccggaac tttccgcagc
    13201 cacggcctta tcatgcttgc atccggcaac taactttgga taatgcccgt tttcagaaca
    13261 ctttcacaag cgactaaatc tttatgaaaa acgacgtcat ttcaccggaa tttgatgaaa
    13321 acggccgccc actgcgccgt atccgtagtt ttgtgcgccg ccaggggcga ctgaccaaag
    13381 gccaggaaca tgcgctggaa aactactggc cggtgatggg cgttgagttc agcgaagata
    13441 tgctggattt ccccgcgctt tttggccgtg aagcgccggt gacgcttgag attggttttg
    13501 gcatgggggc gtcgctggtg gcaatggcta aagatcgccc cgagcaggac ttcctcggca
    13561 ttgaagtgca ttcaccgggc gttggtgcgt gcctggcttc tgcgtatgaa gagggcttaa
    13621 gcaacctgcg cgtgatgtgt cacgatgcgg ttgaagtgct gcataaaatg attcctgaca
    13681 attcattgcg catggtgcag ctctttttcc ctgacccgtg gcacaaagcg cgccataata
    13741 aacgccgtat cgttcaggtg ccgtttgccg aactggtaaa aagcaaactg cagctggggg
    13801 gcgtattcca tatggcgacc gactgggaac cttatgcgga acatatgctt gaagtgatgt
    13861 cttctattga cggttataaa aacctgtcag agagcaatga ttacgtaccg cgtccggcat
    13921 cacgtccagt gacgaaattt gaacaacgtg gtcatcgtct tggtcacgga gtatgggact
    13981 taatgttcga gagggtgaaa taatggcaaa gaaccgtagc cgtcgtctgc gtaaaaaaat
    14041 gcacatcgac gaattccagg aattaggatt ttcggtggca tggcgattcc cggaaggtac
    14101 atcggaagaa cagattgata aaaccgttga tgattttatt aacgaggtca tcgaaccgaa
    14161 caaactggcc tttgacggca gcggttatct ggcctgggaa ggtctgatct gcatgcagga
    14221 aatcggcaaa tgcaccgaag aacatcaggc gattgtgcgt aagtggctgg aagagcgcaa
    14281 actggatgag gtacgcacca gcgaactttt cgacgtttgg tgggactaag aaagcatacg
    14341 ggcgatgaca aatgcaaaac tgcctgatgc gctacgctta tcatgcctgg aaagatgcac
    14401 gatcgagtag gcgggataag gtgtttacgc cgcatccggc atggaaaacg cgtactttgt
    14461 tatcaatctg gggccagcaa atgctggcct gattttttct tgagggaaga ctatgatgcg
    14521 caaaatgctg ctggcggcag cactttcagt gacggcaatg accgctcacg ccgactacca
    14581 gtgcagcgtc acgccgcgtg acgatgtgat tgtcagcccg caaaccgtgc aggtgaaggg
    14641 cgaaaacggc aatctggtga tcacgccaga cggcaacgtg atgtataacg gtaagcaata
    14701 ttccctgaat gccgcccagc gcgagcaggc gaaggattat caggctgaac tacgcagcac
    14761 gctgccgtgg attgatgaag gcgcgaaaag ccgcgtcgaa aaagcccgta ttgcgctgga
    14821 taaaattatc gttcaggaga tgggcgaaag cagcaaaatg cgcagccgtc tgaccaaact
    14881 tgatgcgcag ctgaaagagc agatgaaccg cattatcgaa acgcgcagcg atggcctgac
    14941 gtttcactat aaagccattg atcaggttcg cgccgaaggc cagcaattag tgaatcaggc
    15001 aatgggtgga attttacagg acagcattaa tgaaatgggc gcgaaagcgg tgttgaaaag
    15061 cggcggtaac ccattacaga atgtgctggg aagcctgggg gggctgcaat cctcaatcca
    15121 aaccgagtgg aaaaagcagg aaaaagattt ccagcagttt ggcaaagatg tttgtagccg
    15181 cgttgtgact ctggaagata gccgcaaagc cctggtcggg aatttaaaat aatcctctat
    15241 tttaagacgg cataatactt ttttatgccg tttaattctt cgttttgtta cctgcctcta
    15301 actttgtaga tctccaaaat atattcacgt tgtaaattgt ttaacgtcaa atttcccata
    15361 cagagctaag ggataatgcg tagcgttcac gtaactggag gaatgaaatg gagtttttca
    15421 aaaagacggc acttgccgca ctggttatgg gttttagtgg tgcagcattg gcattaccca
    15481 atatcaccat tttagcaacc ggcgggacca ttgccggtgg tggtgactcc gcaaccaaat
    15541 ctaactacac agcgggtaaa gttggcgtag aaaatctggt taatgcggtg ccgcaactga
    15601 aggacattgc gaacgttaaa ggcgagcagg tagtgaatat cggctcccag gacatgaacg
    15661 ataatgtctg gctgacactg gcgaaaaaaa ttaacaccga ctgcgataag accgacggct
    15721 tcgtcattac ccacggtacc gacacgatgg aagaaactgc ttacttcctc gacctgacgg
    15781 tgaaatgcga caaaccggtg gtgatggtcg gcgcaatgcg tccgtccacg tctatgagcg
    15841 cagacggtcc attcaacctg tataacgcgg tagtgaccgc agctgataaa gcctccgcca
    15901 accgtggcgt gctggtagtg atgaatgaca ccgtgcttga tggccgtgac gtcaccaaaa
    15961 ccaacaccac cgacgtagcg accttcaagt ctgttaacta cggtcctctg ggttacattc
    16021 acaacggtaa gattgactac cagcgtaccc cggcacgtaa gcataccagc gacacgccat
    16081 tcgatgtctc taagctgaat gaactgccga aagtcggcat tgtttataac tacgctaacg
    16141 catccgatct tccggctaaa gcactggtag atgcgggcta tgatggcatc gttagcgctg
    16201 gtgtgggtaa cggcaacctg tataaatctg tgttcgacac gctggcgacc gccgcgaaaa
    16261 acggtactgc agtcgtgcgt tcttcccgcg taccgacggg cgctaccact caggatgccg
    16321 aagtggatga tgcgaaatac ggcttcgtcg cctctggcac gctgaacccg caaaaagcgc
    16381 gcgttctgct gcaactggct ctgacgcaaa ccaaagatcc gcagcagatc cagcagatct
    16441 tcaatcagta ctaatcacct cgccccggta tcgtgccggg gctttttcac ttcagactta
    16501 cgtccattgc caattttcat taccctaatg ataatcaccg gaataaatta ttccgcgtga
    16561 ggtttttcgg gtgaaaaagc aatggattgt tggcacggca ctgcttatgt tgatgactgg
    16621 taatgcctgg gcagatggcg aaccgccaac tgaaaatatc ttaaaagatc aattcaaaaa
    16681 gcagtatcac ggcattctca agcttgatgc tatcacctta aaaaatcttg atgctaaggg
    16741 taatcaggcc acctggtcag cggaaggcga tgtctcttcc agtgacgatc tctatacctg
    16801 ggtcggtcag ttggcagatt acgaactgct cgaacagacc tggacgaaag ataaaccggt
    16861 aaaattctcg gcgatgttaa ccagtaaagg aacgcccgcg tctggctggt cggtgaactt
    16921 ttactctttt caggcggcag ccagcgatcg tgggcgggtg gtcgacgata tcaaaacgaa
    16981 taataaatat ctgatcgtga atagcgaaga tttcaattat cgctttagtc agcttgaatc
    17041 tgcgttgaat acccagaaga actcgattcc tgcgctggaa aaggaagtga aagcgcttga
    17101 taagcaaatg gtggcggcgc agaaagcagc ggatgcatat tggggaaaag atgcaaacgg
    17161 taagcaaatg acccgcgaag aagcctttaa aaaaattcac caacagcgtg atgagtttaa
    17221 taaacagaac gatagcgagg cgttcgcggt taaatatgac aaagaggttt atcaaccggc
    17281 gattgcggca tgtcataaac agagtgaaga gtgttatgaa gtgccgattc agcagaaacg
    17341 agatttcgat atcaacgaac aacggcgaca gacttttctg caatcacaaa aacttagccg
    17401 taaattgcag gatgactggg taacgctcga aaaagggcaa tatcctttaa cgatgaaagt
    17461 ttcagaaata aacagtaaaa aagtcgcgat tttgatgaag attgacgata tcaaccaggc
    17521 taatgaacgc tggaaaaaag ataccgaaca actgcggcgc aatggggtga ttaagtaagt
    17581 ttgatatagt gcacgatttg tagcaggatg cggcgttgac cccgcatccg gcaatcaacg
    17641 tctgatgcga cgcttgcgcg tcttatcaga cctacaaccg ttgccacatg taggctggat
    17701 aaggcgttca cgccgcatcc ggcaatacaa gtttactcag ccagaaaaag ctccagcagc
    17761 gaatttaaaa acagcttccc atgttccgtt atctgccagt aatccgcaca ttcggtgaga
    17821 taaccctggg caatcgcctc gtctaactgt gggcgaatca catcttcgca aagcccggta
    17881 tacgcaataa actccacgcg cggcgcggcc tccagcagac ggaagcgatt cataaagaac
    17941 tcaaacggct tatctgcggc ttcgacatca cgctggcttt ccagatacct tccttgcata
    18001 aaaccacgcg gatgacgcgt tttagtggta cgcagaatgc gcccatccgg gaaggtcact
    18061 ttgccgtgcg cgccgcagcc aataccgatg tagtcgccaa agcgccagta gttgagattg
    18121 tgctggcact gataactggg tttggcgtaa gcggaagttt catattgctg ataacccgct
    18181 gcggttaata actgatgccc ctgttcgaaa atatcccaca gcgcgtcatc gtccggcagc
    18241 accggtggtc gcgaaccaaa cagcgtattg ggttcgatgg tcagttgata ccaggaaaga
    18301 tgcggcggat tcagttcaat ggcctggcgt agatcgccaa gcgcctcttc cagtgattga
    18361 tccggtagtc catgcatcaa atcaaggtta aagctacgta accctaaacc gctcgccagc
    18421 tttgccgctc gtttcgcttc ttgcgggcca tgaatgcgcc cgagtcgttt cagcttttct
    18481 tcgctaaaac tctgcacacc aatagagatg cggttcacac cagcacgctg ataatcgaca
    18541 aagcgatccg cttctaccgt gcctgggttc gcttccatag taatttctgc atccgctgcc
    18601 agcggcaaac gcgcacgcac gccgtccagc agcgtttgca tcgccgggcc ggaaagcagg
    18661 ctcggcgtac caccgccaat aaagattgtc tttacttcac ggccctgagc gtaagccaca
    18721 tcgttgtcca gatcgttaag cagatgctga acataatcgt cgtgcggcac ttctcctttc
    18781 aacgcgtgag agttgaaatc gcagtacggg catttctgca cgcaccacgg gatgtgaatg
    18841 tagagactca gcggcggtaa tttaaccatt acgtaaagcg tccagcagca gtttcaatgc
    18901 ctgaccacgg tgggaaatgg cgctcttttc ttcgcgggtc agttcggcgg cggttttccc
    18961 ttcggaaggt acgaagaaga ttggatcata accaaagcca ccagtgcccg ctggttcacg
    19021 agtaatcacg cccggccagc taccgtggca taccagcgga gtcggatctt ccgcgtgacg
    19081 cagatatacc agcacgcagt ggaaacgcgc ctgacgttgg tcatccggta cgtctttcag
    19141 tgtttccagc agtttttgca gattctttag atcggtcgcg tcttcaccgg aataacgcgc
    19201 ggagtaaatg cccggcgcgc cgccaagcac atctaccgcc agaccagagt cgtcggcaat
    19261 tgccggtaaa ccggtcactt ttgccgcatg gcgcgctttc agaatcgcgt tttcgataaa
    19321 ggtcaggccg gtttcttcag cggaatctac gccgaggtct gtttgggcca cgatatcaag
    19381 accgaagtcg ctaagcagcg acgccagctc acgcacttta ccggcattgc cggttgcgag
    19441 gacaactttt tgcataggat acctaattaa ttaacgccgc gatttctggc gggatttgtt
    19501 gcggattaat gattttaatt tgtttgtggc ggccaagttc gcctttttca atcaccacct
    19561 ggcttttggc aacccggaat tgcttaccga gaaacttcac cagatgactg ttggcctgac
    19621 cgtcaaccgg cggcgcggta atggcgactt taacttcgtc gccatgtaaa ccgacaatag
    19681 aatcacggct ggctttcggc tgaatataga gccgtaaaac cagaccgtca tcattaactg
    19741 ttacggcact cataacgcca tccacagccc cggcagcagc atgtttccgg ttgcctgtaa
    19801 tacttctgcg acacccatat tgatgacata cagcagcaga acgaggatca tcggcgagaa
    19861 atcaatccca cccattgccg gtagcaggcg gcgaatcggg cgcagcagcg gatcggccag
    19921 ctgaatcagc acgtattcaa tcggactacg accctggctt acccagctca taatcgccat
    19981 caccagcagg acccagaaaa tcagcaggcc gatggttttc agcagaatca gtaaaccggc
    20041 aatccagatg attggcagga aggtcaccac tttaaacagc acgatggctt tgataaaact
    20101 gagaatgtac gcaaccagca gcgaggcgct atcaatggga cccattgccg gaataacgcg
    20161 gcgcagtggc ccgataattg gctgtgttac cttcactaca aactgtgaga aggggttgta
    20221 aaaatcacaa tgagcccact gcatccagat gcgtaataac agcaccatgg tatacagctc
    20281 aatgaccgtt gaaagcagga aagtcaacgt attcatggcg ttcctttaat tccttatttt
    20341 ttagagtaat cacgcgcacc aaaaattgca gtgccgatac gaaccatcgt gctacctgcc
    20401 gcaatggcgg cttccatatc gtccgacatt cccagagaga gcgtgtcgat atgcgggtag
    20461 cgcgttttca gtccggcaaa tgctacagcc atttggcgtg caacttcaaa ctgccttaca
    20521 tattctgact caggcgcagg gattgccatc agcccgcgca gacgtaaacg cggtagttca
    20581 gcgaccgccg ctgccagctc gtccagttct gccagttgaa tcccggactt actgttttca
    20641 tcactaatat taatttgaat cagtacgtta agagggggaa gttctgccgg gcgctgatcg
    20701 ttgaggcggg tagcaatgcg caaacggtcg atggtatgac accagtcgaa atgctctgcc
    20761 accaggcggc ttttattaga ctgcaacggg ccaataaaat gccattctaa tcctgttacg
    20821 cccagttcct gaaagtggcg aattttatct accccttcct gaacgtagtt ttcaccaaat
    20881 tgacgctgcc cggcatcaat ggcttctgcg atggcgctcg caggttttgt tttactgact
    20941 gcaagcagcg taatttcttc tggagaacgg ccgcaacgcg ttgcagccgc tgagattttg
    21001 tcccggacct gtgccaggtt atgcgcaata tcgttcattt tccgaggatc ttagtatgaa
    21061 tatggaagaa attgtggccc ttagtgtaaa gcataacgtc tcggatctac acctgtgcag
    21121 cgcctggccc gcacgatggc gcattcgcgg gctaatggaa gctgcgccgt ttgatgcgcc
    21181 ggacgtcgaa gagctactgc gggagtggct ggatgacgat cagcgggcaa tattgctgga
    21241 aaatggccag ctggattttg ccgtgtcgct ggcggaaaac cagcggttgc gtggcagtgc
    21301 gttcgcgcaa cggcaaggta tttctctggc attacggttg ttaccttcgc actgtccaca
    21361 gctcgaacag cttggcgcac caacggtatt gccggaatta ctcaagagcg agaatggcct
    21421 gattctggtg acgggggcga cggggagcgg caaatctacc acgctggcgg cgatggttgg
    21481 ctatctcaat caacatgccg atgcgcatat tctgacgctg gaagatcctg ttgaatatat
    21541 ctatgccagc cagcgatgtt tgatccagca gcgggaaatt ggtttgcact gtatgacgtt
    21601 cgcatcggga ttgcgggccg cattgcggga agatcctgat gtgattttgc tcggagagct
    21661 gcgtgacagc gagacaatcc gtctggcact gacggcggca gaaaccgggc atttggtgct
    21721 ggcaacatta catacgcgtg gtgccgcgca ggcagttgag cgactggtgg attcatttcc
    21781 ggcgcaggaa aaagatcccg tacgtaatca actggcaggt agtttacggg ccgtgttgtc
    21841 acaaaagctg gaagtggata aacaggaagg acgcgtggcg ctgtttgaat tattgattaa
    21901 cactcccgcg gtggggaatt tgattcgcga agggaaaacc caccagttac cgcatgttat
    21961 tcaaaccggg cagcaggtgg ggatgttaac gtttcagcag agttatcagc agcgggtggg
    22021 ggaagggcgt ttgtgacgtt gccatgcccg atgcgcaagt ttatagggcc tataaaggca
    22081 ataaatgtct gatgcgacgc taacgcgtct tatcagacct acagccgctg ccagatgtgt
    22141 gctggataag gcgttcacgc cgcatccggc aagatggcgg taacgactat ccgccaggct
    22201 tttagatcgc cttaatatcc ctgctcgaaa tagctttcga gaataataac cgcagaggca
    22261 gagtcgactt tgcctttgtt gagcgcccga tagccgccct gttcaaacag accggaacgg
    22321 gcttccacgg tgctaagacg ttcgtcatgg agctttactt caacaccgaa acgaccatga
    22381 atacggttgg caaatttacg cgctctggca gtcaatggtt gctcggtgcc gtccatattc
    22441 agcggcaaac cgacgatgat ttcgtccggc tgccactctt tcagtaaacg ttcgatgagg
    22501 ttccagtccg gcgtaccgtc ctgtgcttta attgcaggca aagggcgagc agtgccggta
    22561 atgcgttggc cgaccgctac gccaatgctt ttggtgccga agtcgaaggc gagtaaggtt
    22621 ccactcatca ggcgtgccct gccacaccag gcatggtgag aatatccaca ccaatcagtt
    22681 ttgccgcctc gcgccagcgg tcggcaatcg gcgttttgaa cagaatattc agatccgccg
    22741 gggccgttag ccacgcgtta tcgagaattt cttgttccag ttgacctttt tcccaggagg
    22801 cataacccag agccaccaat acgtcagacg gttgtttatc ggtgccgagc gtttccagca
    22861 catcgcggga agtggtcatc actgtgttgt ctgaaatgcg aatgctggaa gcaaaattgg
    22921 agggcggagt atgcaaaata aacccgcgat cttcagccag cggaccgccg agcataaccg
    22981 gtttatccag acggattgat tcatcacgcg gctccggcgt gatcttcagc ttttccagaa
    23041 tcccttcaat tttgagattt tccagcggct tgttgacgat gatccccatt gcaccattgg
    23101 tattatgttc gcaaatgtag accacggaac gacggaaaat cggatcctgg agagcaggca
    23161 tggcaataag aaagtgatgc tgtaaattca ttgtcagagg ttctgtttcc tggttcaaaa
    23221 aagcaacagc gcccagtatg cgtggaaaga agggcgctgt cactcagagt ctcaacgaga
    23281 tccttctcgc taaggtgggt tactgctgct gtaaacgtgc ttcgatggca tccattaaca
    23341 ttccggtgat cgacaccgga aactctgctt caatctcacg aatacaggtt gggctggtga
    23401 cgttaatttc agtcagacgg tcgccgatga tatccagacc aacaaaaatc agcccttttt
    23461 ctttcagcgt cggcccgatc tgacgggcga ttttccagtc actttccgtc agcggacgag
    23521 gttcaccgcg accaccggca gccagattgc cacgggtttc gcccccctgc ggaatacgcg
    23581 ccaggcagta cggtaccggc tcgccatcca ccaccagcac gcgtttgtcg ccatctttaa
    23641 tggctggcag gtaattttgc gccatgcagt agcgagtgcc atgctcagtc agggtttcgg
    23701 caatcacgcc gaggtttgga tcgccttctt tcacgcggaa aatcgacgcg ccgcccatac
    23761 cgtccagcgg cttaagaatg atgtcgctgt gtttctccca gaacgctttt agctgcgctt
    23821 tattgcgcgt aaccagcgtt tctggcgtta agtcagagaa ccaggcggta aacagtttct
    23881 cgttacagtc gcgcaggctc tgcggcttgt taacgatcag cgtccctttc tcttcggcac
    23941 gttccagaat ataggtcgcg tagataaact cggtatcaaa cggcgggtct ttacgcatca
    24001 ggatcacatc gagatcggcc agcggcagat cctgttcacc gacgaacgaa aaccactctt
    24061 cgtagttctg cttcacgttc agcgtgcggg tatgggcgcg ggcttcgcca ttgatcagat
    24121 acagatctcc catctccata tagtgaagtt cgtaaccacg acgctgtgct tccagcaaca
    24181 tagcgaaact ggaatctttc ttgatgttga tgtttgcgat ggggtccatc acgatgccga
    24241 gcttgatcat tattcttctc cgttagccca aatcgccaaa tcgtacttgt agcgcggtaa
    24301 tggcggtgag cgcagttgtc tctgtacgca aaacgcgagg tcccaacagg atatcagtaa
    24361 attgatagcg ggcagtcatg gcaatttcat ctgccgataa accgccttcc gggccaatca
    24421 gcaggcggac gcgttcaacc ggtaacggca acgtattgat gctgttactg gcgcgcgggt
    24481 gaagattcag tttcagtcct tcatcctgct ctgcacacca ggcttccaga tccatcgctg
    24541 ggcggatttc cggcacccgg ttacgaccac actgctcaca ggcagcaatt gcaatcttct
    24601 gccactgctg gagcttcttg ttcagacgtt cactatccag tttaacgccg cagcgctcag
    24661 aaaaaagtgg cgtaatgagg cttacaccga gttcgatcga tttctggatc gtaaattcca
    24721 ttttttcacc acgcgacatc acctgaccga ggtgaatatg cagcggagat tcgcgatcgt
    24781 cgatcttgcc ttccagcacc ttcacttcca cgcttttttt gctggcgctg gtaatttcgg
    24841 cgtcaaagac ctggttgcta ccgtcaaaca attgcaacgc ctgccccggc cccatgcgca
    24901 gtacgcgccc gatatggttg gcggcatctt cgcaaagcgc gatgtgagaa tggctggtca
    24961 gtggttcagg atgataaatg cgggggatac gcatagttaa aaatccgcgt cgtctcccca
    25021 cgcggttgta cgcgtggggt aggggttaac aaaaagaatc ccgctagtgt aggttagctc
    25081 tttcgcgcct ggcaagcgcg ttgcacatac gggttatgat tgccttgcac cttcgcgatg
    25141 cgttcatcgc gctcgcactc ccagtcggta accggataca tcttgttcca tgcgttgaac
    25201 agctgcgttt gctggcgaga gagtgtcagg ttgtattggt cgcgcatata gaagtaggtg
    25261 cgcgcaatgg caccgcgtgc acgcgctggt ggttcggcag ctttttcttt gaaatcgacc
    25321 ttcatggcgc attgaccgta ctggccttcg ccgccattcc actggctgta cataaagttg
    25381 ccgcgatcgc cattcacctc accgactgac ggctgcaggt tatgcatatc gctttccatc
    25441 ttgcgataga ccggatcttt agcgcagttt ttacgtccac cgtcctgcca gcactggcgc
    25501 tggtgaccga actgccaggc gggaacgacg tgttcccact ctacgcggct ggctcggttt
    25561 tcatttttgc gcacctgata gccgcacgat tgcagatcaa caacgccttt tttgccctgc
    25621 cagttaattt tacatccgca ataaaacgta ccgggcgcgt cagcgtggac ttttaccgcc
    25681 gcggctttcg cctgagaaaa actattgata ccttcggcca acgccgggcc ggaaaatgct
    25741 gcgctcagta ccaccgcagc aatagacaaa taacggtaca tcacttactc cgtgttaaaa
    25801 cgagccagca acgtagcgaa agttgttttg gtatgcaatc agccagatca ggaaagttcc
    25861 tgatagttca gatggttatt tcgcaaccag ctgttcaccg cagtgaacac agcgatagac
    25921 ggcctcgcca cgaactacgc gattatggcg gcgtacggta agctgatgct cctggcactt
    25981 gcagcggtag gggaaggtgt tgcgacgcac ggattgcagt tcgaactgat gcgtacgacg
    26041 ggcgggaacg cccagcacgc tttccatcat ccacttccac tctttgccat gtggcgctac
    26101 gcggccgaaa tgtttccata ccagcaaatg tgccagttcg tgcggtacca cttcttcaat
    26161 aaaagcttca ctgttttcca acagcaaaac gggattgagg cggatttcat agctttccag
    26221 ccaggctgtt ccggcggagg ttccgcgctg ggtgtaagag agttttggct ccgggtagtt
    26281 acgccctagc ttcaggttgg cctgggcgag tttttcccgc aggcggcgca taacggcctg
    26341 ctggatggcg atagggagac gggatgtttt catagcggca gaggatagag cgaagaaaag
    26401 atgactgcaa gaggtggctt cctccgcgat gggaggaagc ctgaggagat taatcgtgag
    26461 cgccgatttc gcgcagttta cgacctttca tcagattacg ttcaatatgt tccagcgaaa
    26521 cgtgtttggt ttccggtacc agccacaatg tcagcaggat aaacagtacg ttcagagccg
    26581 catacaccca gaaggtgttg gcgttaccca gcgtgttgag catggtcagg aacgttgcgc
    26641 caacgatcat gttggcaatc cagttggtgg cggtggagca ggtgatgcca aaatcgcggc
    26701 ctttcagcgg ctgaatttcg gagcacagta cccaaatcag cggaccggca ctcatggcaa
    26761 aaccgacaat aaacatcagc agcatggcga tggcgaaata ctgcgctgac ggagagtgaa
    26821 taccgatatg catcattgta ccgagtacgc ccatgccagc tgccatcacc aggaagccca
    26881 gcgttagcgt tggtttacgt ccccagcggt caacaaggcc gattgcgata aaggtggcaa
    26941 gtacgttggt caggccgaca atcacggtcc cccacatttg ctcggtagtg ttggtataac
    27001 ccgccagttc gaagattttc ggcgcgtaat acatgatgac gttcatcccg gtgaattgct
    27061 gcattacctg caacagtacg ccaaggaaca ccgcgcggcg gaagttgctg ttctctttaa
    27121 acagcgccca gccactctgt ttaacctgca aactttcacg gatttcatcc agttcgcgtt
    27181 tcgcttccgc gctggtgtca cgcaggcgta gcagcacgcg ttcggcatca acaaaacggc
    27241 gcttggcggc aaaccaacgt gggctgtctg gcaggaagaa gacaccaatc agcagcaaaa
    27301 ttgccgggat gataatcaca cccagcatcc agcgccatgc accggtgtag ctgaaggcgg
    27361 tatcagaaag ataagcaccg aggatcccga tagtgatcat caactgatac atcgagatca
    27421 tactgccgcg aattttttcc ggcgcaattt ctgacaggta cagtggtgcg gtataagagg
    27481 ccacacccac cgccagcccc agcagaacgc gggaaagaat cagtacttca acgtttggcg
    27541 cagccgcaga gaacagcgaa ccggcaacaa acaaaattgc gccgatcatc aggctctttt
    27601 tgcgcccgag tttaaaggag agccagccgc tgcccaccgc accgactgcc gcaccgaaca
    27661 tcatggagct tacgacccat tcttgcgtat gcgaagtaat ctggaattca tctgcaataa
    27721 acggcagtgc gccagcaatt acaccgatat ccaggccaaa gagtaatccc gccagagcgg
    27781 caaggaagca gacgaaaaac gtcattgcct tgtttgaccg cccctgtttt ttagcgtcag
    27841 gcatgatgcc ctccaatatg gttatttttt attgtgaatt aagataggtg agtacgacgt
    27901 aaaaagatgt gaagcaaatc acatcagtgt aatcgcttac actaacattt atcttagtta
    27961 tggtttaag
//
LOCUS       DAPHDW010000052        27412 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.052, whole genome shotgun sequence.
ACCESSION   DAPHDW010000052 DAPHDW010000000
VERSION     DAPHDW010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27412
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.052"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(45..1295)
                     /gene="icd"
                     /locus_tag="RH057_002203"
     CDS             complement(45..1295)
                     /gene="icd"
                     /locus_tag="RH057_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HDV3128565.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1467..2120
                     /gene="rluE"
                     /locus_tag="RH057_002204"
     CDS             1467..2120
                     /gene="rluE"
                     /locus_tag="RH057_002204"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HDV3128566.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKPENQPTRVILF
                     NKPYDVLPQFTDEAGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2130..2591
                     /gene="nudJ"
                     /locus_tag="RH057_002205"
     CDS             2130..2591
                     /gene="nudJ"
                     /locus_tag="RH057_002205"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HDV3128567.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILKASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2645..3751
                     /gene="mnmA"
                     /locus_tag="RH057_002206"
     CDS             2645..3751
                     /gene="mnmA"
                     /locus_tag="RH057_002206"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HDV3128568.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGIEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIEVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3787..4428
                     /gene="hflD"
                     /locus_tag="RH057_002207"
     CDS             3787..4428
                     /gene="hflD"
                     /locus_tag="RH057_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HDV3128569.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSRQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4432..5802
                     /gene="purB"
                     /locus_tag="RH057_002208"
     CDS             4432..5802
                     /gene="purB"
                     /locus_tag="RH057_002208"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HDV3128570.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQYRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            5970..6641
                     /gene="phoP"
                     /locus_tag="RH057_002209"
     CDS             5970..6641
                     /gene="phoP"
                     /locus_tag="RH057_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HDV3128571.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6641..8101
                     /gene="phoQ"
                     /locus_tag="RH057_002210"
     CDS             6641..8101
                     /gene="phoQ"
                     /locus_tag="RH057_002210"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HDV3128572.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAKWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTFVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            8177..9298
                     /gene="roxA"
                     /locus_tag="RH057_002211"
                     /gene_synonym="ycfD"
     CDS             8177..9298
                     /gene="roxA"
                     /locus_tag="RH057_002211"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HDV3128573.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(9347..10573)
                     /gene="pepT"
                     /locus_tag="RH057_002212"
     CDS             complement(9347..10573)
                     /gene="pepT"
                     /locus_tag="RH057_002212"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HDV3128574.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVESYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVDRADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVTLEGMEK
                     AVQVIVRIAELTAQRK"
     gene            10823..11959
                     /gene="potA"
                     /locus_tag="RH057_002213"
     CDS             10823..11959
                     /gene="potA"
                     /locus_tag="RH057_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HDV3128575.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMREGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            11943..12800
                     /gene="potB"
                     /locus_tag="RH057_002214"
     CDS             11943..12800
                     /gene="potB"
                     /locus_tag="RH057_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HDV3128576.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            12797..13591
                     /gene="potC"
                     /locus_tag="RH057_002215"
     CDS             12797..13591
                     /gene="potC"
                     /locus_tag="RH057_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HDV3128577.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            13588..14634
                     /gene="potD"
                     /locus_tag="RH057_002216"
     CDS             13588..14634
                     /gene="potD"
                     /locus_tag="RH057_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HDV3128578.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            14692..15153
                     /locus_tag="RH057_002217"
     CDS             14692..15153
                     /locus_tag="RH057_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001460774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HDV3128579.1"
                     /translation="MSQDSKVLSRAFLGIGLILLVISAIIFYYHFTFTKSAVHTEGII
                     VDAVWYNNHSNDVDDNGSWYPVVAFRPTPDYTLIFNSNIGSDFYEDSEGDRVNVYYPP
                     GHPEQAEINNPWVNFFKWGFVGIAGIIFGSVGLIITLPSTKKSRRKRKSRP"
     gene            15150..15575
                     /locus_tag="RH057_002218"
     CDS             15150..15575
                     /locus_tag="RH057_002218"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HDV3128580.1"
                     /translation="MKKFIILLSLLILLPLVATSKPLIPIMKTLFTDVTGTVPDAEEI
                     ARKAELFRQQTGIAPFIVILPDINNEASLRQNGKAMLAHASSSLSNVKGSVLLLFTTR
                     EPRLIMITNGQVESGLDDKHLGLLIENHTLAYLNADLWY"
     gene            15591..15938
                     /locus_tag="RH057_002219"
     CDS             15591..15938
                     /locus_tag="RH057_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128581.1"
                     /translation="MAVLQAQILKQPTPPLTYYPHPGQQHENAPPGSTNTLGFIAWAA
                     TFILFSWIFCYTTRFIYALKFAVAMTIANMGYQALCLYIDNSFAITRITPLWAGLIGV
                     CTFIAALLLTSKR"
     gene            complement(16077..16898)
                     /gene="cobB"
                     /locus_tag="RH057_002220"
     CDS             complement(16077..16898)
                     /gene="cobB"
                     /locus_tag="RH057_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HDV3128582.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGL"
     gene            complement(16914..17825)
                     /gene="nagK"
                     /locus_tag="RH057_002221"
     CDS             complement(16914..17825)
                     /gene="nagK"
                     /locus_tag="RH057_002221"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HDV3128583.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGTLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLIFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVCLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPRHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(17854..19098)
                     /gene="lolE"
                     /locus_tag="RH057_002222"
     CDS             complement(17854..19098)
                     /gene="lolE"
                     /locus_tag="RH057_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HDV3128584.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVDQPWTNWQEALDHVQKVPGIAAAAPYINFTG
                     LVESGVNLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHVAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VIIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(19098..19799)
                     /gene="lolD"
                     /locus_tag="RH057_002223"
     CDS             complement(19098..19799)
                     /gene="lolD"
                     /locus_tag="RH057_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HDV3128585.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLHNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSRALEMLKAVGLDHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(19792..20991)
                     /gene="lolC"
                     /locus_tag="RH057_002224"
     CDS             complement(19792..20991)
                     /gene="lolC"
                     /locus_tag="RH057_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HDV3128586.1"
                     /translation="MYQPVALFIGLRYMRGRAADRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAGN
                     ITGWRLWLDEPLKVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            21253..22326
                     /locus_tag="RH057_002225"
     CDS             21253..22326
                     /locus_tag="RH057_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV3128587.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHLTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDIRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLLYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            22454..25900
                     /gene="mfd"
                     /locus_tag="RH057_002226"
     CDS             22454..25900
                     /gene="mfd"
                     /locus_tag="RH057_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HDV3128588.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGVLIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANTNLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKVRNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARILLDVARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            26044..27006
                     /gene="ldtC"
                     /locus_tag="RH057_002227"
     CDS             26044..27006
                     /gene="ldtC"
                     /locus_tag="RH057_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HDV3128589.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPAGSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRADSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(27089..27346)
                     /gene="bhsA"
                     /locus_tag="RH057_002228"
     CDS             complement(27089..27346)
                     /gene="bhsA"
                     /locus_tag="RH057_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple stress resistance protein BhsA"
                     /protein_id="HDV3128590.1"
                     /translation="MKNVKTLIAAAILSSMSFASFAAVEVQSTPEGQQKVGTISANAG
                     TNLGSLEEQLAQKADEMGAKSFRITSVTGPNTLHGTAVIYK"
BASE COUNT         6673 a         7106 c         6945 g         6688 t
ORIGIN      
        1 cgggcgtgtt atacgcccgt tgcaatatta aacacatgta gtgattacat gttcttgatg
       61 atcgcatcac caaattctga acatttcagc agtttagcgc cttccatcag acgctcgaag
      121 tcataggtta cggtcttcgc attgattgcg ccttccatac ctttaacaat caggtctgcg
      181 gcttcagtcc agcccatatg gcgtaacatc atctcagcgg agagaataat agagccaggg
      241 tttactttgt cctgaccggc gtatttcggc gcagtaccgt gggtggcttc aaacagggcg
      301 cattcgtcac cgatgtttgc accaggggcg ataccgatac cgccaacctg cgctgccagg
      361 gcgtcagaaa tgtagtcacc gttcaggttc atacaggcga taacatcata ttcagccgga
      421 cgcagcagga tctgttgcag gaatgcatca gcaatcacgt ctttaatgac gatctctttg
      481 ccggtgttcg ggtttttaac tttcagccac gggccgccgt cgatcagttc accgccaaac
      541 tcttcacgcg ccagctggta gccccagtct ttaaacgcgc cttcggtgaa cttcatgatg
      601 ttgcctttgt gcaccagggt cacagagtca cggtcgttgg caattgcgta ttcaatcgcg
      661 gcacggacca gacgtttggt gccttcttca gaacacggct tgataccgat accgcaatgt
      721 tccgggaagc gaattttctt cacgcccatc tcttcacgca ggaatttaat cactttctcg
      781 gcgtcggcag agtctgcttt ccattcgata cccgcataaa tgtcttccga gttttcacgg
      841 aagataacca tatcggtcag ttcagggtgt ttaaccgggc ttggagtgcc ttgatagtaa
      901 cgtaccggac gcaggcagat gtagagatcc agttcctggc gcagggcaac gttcagagag
      961 cgaataccgc caccaacagg agtggtcagc ggacctttaa tggcaacgcg atattcacga
     1021 atcagatcaa gggtttcagc aggcagccag acatcctgac cataaacctg tgtggatttt
     1081 tcaccggtgt aaatttccat ccaggagatt ttacgctcgc ctttataggc tttctcgact
     1141 gcagcgtcga ccactttcag catggctggg gttacatcta caccgattcc atcaccttca
     1201 atgtaaggga taatcggatt ttcaggaacg ttgagtttgc cgttttgcag ggtgatcttc
     1261 ttgccttgtg ccggaacaac tactttactt tccattcacc tctccttcga gcgctactgg
     1321 tttgctcgtc tgccaccacg ttgcatatgc gtttgcgtcc tgcgatacgg atgctttaga
     1381 gcaatttttt gttaatgatt tgtaattggc ttgtcaatac taccctattg tttgccgtca
     1441 tgaaagatgc gcgttattag gctataatgc ggcaattcat aatctctgaa aataccatgc
     1501 aaaaaacttc ttttagaaat caccaggtta agcgattcag ctcgcaacgt tctaccaggc
     1561 gtaaacctga aaaccagccc acgcgtgtga tcctgttcaa taaaccctac gatgttcttc
     1621 cgcagttcac cgatgaagcc ggacgcaaaa cattaaaaga attcatcccg gttcagggcg
     1681 tttatgcagc aggtcgtctt gaccgcgata gcgaagggtt actggtgctg accaataacg
     1741 gcgcgttgca ggcgcgttta acccagccgg gtaaacgcac cgggaaaatc tattatgtgc
     1801 aggtggaagg tattcccaca caagacgcac ttgaagcctt gcgcaatggc gtaaccttaa
     1861 atgatggccc taccctgccc gccggcgcgg agctggttga cgaaccagcg tggttatggc
     1921 cgcggaatcc accaattcgt gaacgcaaaa gtattcccac cagctggctg aagatcacct
     1981 tatatgaagg acgtaatcgc caggtgcgcc gcatgaccgc ccatgttggt ttccccacgc
     2041 tgcgactgat tcgctatgcg atgggagatt actctttgga taatcttgcc aatggcgaat
     2101 ggcgagaagt gacagattaa ggatgcaaaa tgtttaaacc gcacgttacc gttgcttgcg
     2161 tggtgcacgc agaaggcaaa tttttagtcg ttgaagagac gattaatggt aaagcgttat
     2221 ggaaccaacc tgccgggcat ctggaagccg atgaaacctt agtggaagcc gccgcccgtg
     2281 aactgtggga agaaaccggc atcagcgcgc agccgcaaca ctttattcgt atgcatcagt
     2341 ggattgcgcc agataaaacg ccgtttttgc gtttcctctt tgccattgag cttgagcaaa
     2401 tatgcccgac ccagcctcat gacagcgata tcgactgctg tcgttgggtc agcgccgaag
     2461 agattttaaa ggcgtcaaat cttcgttcgc cgctggtggc ggaaagtatc cgttgttatc
     2521 aaagcgggca acgctatccg ctggagatga ttggcgattt taactggcct tttacaaagg
     2581 gtgtcatcta aaaggctgcg tgatagaata cgccgccttg aagttcaatg tcgtgagtga
     2641 tccaatgtct gaaaccgcaa aaaaagtaat cgtcggcatg tccggcggtg tcgattcctc
     2701 cgtttctgcc tggctgttgc aacaacaggg atatcaggtt gaaggcctgt ttatgaagaa
     2761 ctgggaagaa gacgacggtg aggaatattg cacagcggca gcggatctgg ctgatgccca
     2821 ggctgtctgc gacaagctcg gcattgaact gcacaccgtt aactttgctg ccgagtactg
     2881 ggacaacgtc ttcgaactgt tccttgccga atataaagcc ggtcgcacgc cgaatccgga
     2941 tattctgtgc aacaaagaga tcaaatttaa agccttcctc gaatttgccg ccgaagattt
     3001 aggtgccgat tatatcgcta ccggtcatta cgtacgtcgg gccgatgtcg atggcaagag
     3061 ccgcctgctg cgtggtctgg acagcaataa agaccagagc tacttccttt atacgctcag
     3121 ccatgagcag attgcgcaaa gcctgttccc ggtcggcgaa ctggaaaaac cgcaggtgcg
     3181 taagattgct gaagatcttg gtctggtcac cgcgaagaaa aaagactcta ccggcatttg
     3241 cttcattggc gaacgtaaat tccgcgagtt cctgggccgt tatctcccgg cgcaaccggg
     3301 caaaatcatt accgtcgatg gcgatgaaat tggtgagcac caggggctga tgtatcacac
     3361 tctcggtcag cgtaaaggtc tgggtatcgg tggcaccaaa gaaggtatcg aagaaccgtg
     3421 gtatgtggtg gacaaagacg tcgaaaacaa cattctggtt gtcgctcagg gccatgaaca
     3481 cccgcggctg atgtctgtcg ggttgattgc ccagcagttg cactgggtcg atcgcgaacc
     3541 attcaccggc actatgcgtt gcacggtaaa aacccgctat cgccagaccg atatcccttg
     3601 caccgtcaag gcgctggacg atgatcgcat tgaagtgatt ttcgatgaac cggttgccgc
     3661 cgtgacgccg ggccagtctg ccgtcttcta taacggtgaa gtgtgcctcg gtggcggtat
     3721 tattgagcag cgtctgccgc tgccggtctg attattatct ttacttaaaa caaggaagca
     3781 gtgaacgtgg caaagaatta ctatgacatc accctcgccc tggccggtat ttgtcagtcg
     3841 gcacgcctgg tgcaacaact cgctcaccag gggcattgtg atgccgatgc gctacacgtc
     3901 tcactcaaca gtattattga tatgaacccc agctcgacgc tggcggtttt tggcggtagc
     3961 gaagccaacc tgcgcgtcgg gctggaaacc ctgctcggcg tgctcaatgc cagcagtcgc
     4021 cagggcttaa acgccgaatt aacccgctac acactcagct tgatggtgct tgagcgcaaa
     4081 ctctcctcag cgaaaggcgc gctcgacact ctgggcaacc ggatcaacgg cctgcaacgc
     4141 cagctcgaac acttcgattt acagtccgaa acgctgatga gcgcgatggc tgctatctat
     4201 gttgatgtga tcagcccgct tggcccgcgc attcaggtca ccggttcccc tgctgtactg
     4261 caaagcccac aagtgcaggc gaaagttcgc gcaaccctgc tggcaggcat tcgcgccgcc
     4321 gtgctctggc accaggtcgg cggcggacgt ctgcaactga tgttttctcg taatcgcctg
     4381 accactcagg caaaacaaat tcttgctcat ttaaccccgg agttgtgatc tatggaatta
     4441 tcctcactga ccgccgtttc ccctgtcgat ggacgctacg gcgataaagt cagcgcgctg
     4501 cgcgggattt tcagcgaata tggtttgctg aaattccgtg tacaagttga agtacgttgg
     4561 ctgcaaaaac tggccgcgca cgcagcgatc aaggaagttc ctgcttttgc tgccgacgca
     4621 atcggttacc ttgatgcaat tgtcgccaat ttcagcgaag aagatgccgc acgcatcaaa
     4681 accatcgagc gtaccactaa ccacgacgtt aaagcggttg agtatttcct gaaagaaaaa
     4741 gtggcggaga tcccggaact gcacgcggtt tctgaattca tccactttgc ctgtacttcg
     4801 gaagatatca ataacctctc ccacgcatta atgctgaaaa ccgcgcgtga tgaagtgatc
     4861 ctgccgtact ggcgtcaact gattgatggc attaaagatc tcgccgttca gtaccgcgat
     4921 atcccgctgc tgtctcgtac ccacggtcag ccagccacgc cgtcaaccat cggtaaagag
     4981 atggcaaacg tcgcctaccg tatggagcgc cagtaccgcc agcttaatca ggtggagatc
     5041 ctcggcaaaa tcaacggcgc ggtcggtaac tataacgccc atatcgccgc ttacccggaa
     5101 gttgactggc atcagttcag cgaagagttc gtcacctcgc tgggtattca gtggaacccg
     5161 tacaccaccc agatcgaacc gcacgactac attgccgaat tgtttgattg cgttgcgcgc
     5221 ttcaacacca ttctgatcga ctttgaccgt gacgtctggg gttatatcgc ccttaaccac
     5281 ttcaaacaga aaaccattgc tggtgagatt ggttcttcca ccatgccgca taaagttaac
     5341 ccgattgact tcgaaaactc cgaagggaat ctgggccttt ccaacgcggt attgcagcat
     5401 ctggcaagca aactgccggt ttcccgctgg cagcgtgacc tgaccgactc caccgtgctg
     5461 cgtaacctcg gcgtgggtat cggttatgcg ctgattgcgt atcaatccac cctgaaaggc
     5521 gtgagcaaac tggaagtaaa ccgtgaccat ctgctggatg agctggatca caactgggaa
     5581 gtgctggctg agccaatcca gacagttatg cgtcgctatg gcatcgaaaa accgtacgag
     5641 aagctgaaag agctgactcg cggtaagcgc gttgacgccg aaggcatgaa gcagtttatc
     5701 gatggtctgg cgttgccaga agaagagaaa gcccgcctga aagcgatgac gccggctaac
     5761 tatattggtc gagctatcac catggttgat gagctgaaat aaactcgtat cagtgccgga
     5821 tggcgatgct gtccggcctg tttattaaaa ttatccactt tttctttttt cactttacct
     5881 cccctccccg ctggtttatt taatgtttac ccccataaac acataatcgc gttacactat
     5941 tttaataatt aagacaggga gaaataaaaa tgcgcgtact ggttgttgaa gacaatgcgt
     6001 tgttacgtca ccaccttaaa gttcagattc aggatgctgg tcatcaggtc gatgacgcag
     6061 aagatgccaa agaagccgat tattatctca atgaacattt accggatatt gcgattgtcg
     6121 atctcggatt gcctgacgag gacggtctgt cactgattcg ccgctggcgt agcaatgatg
     6181 tttcactgcc gattctggta ttaaccgccc gtgaaagctg gcaggacaaa gtcgaagtat
     6241 taagtgccgg tgctgatgat tacgtgacta aaccgtttca tattgaagag gtgatggcgc
     6301 gaatgcaggc attaatgcgg cgtaatagcg gcctggcttc acaggtcatt tcgctacccc
     6361 cgtttcaggt tgatctctct cgccgtgaat tatctattaa tgacgaagtg atcaaactga
     6421 ccgcgttcga atacaccatt atggaaacgt tgatacgcaa taatggcaaa gtggtgagca
     6481 aagattcgtt aatgctccaa ctctatccgg atgcggagct gcgggaaagc cataccattg
     6541 atgtactgat gggacgtctg cgcaaaaaaa ttcaggcaca atatccgcaa gaagtgatta
     6601 ctaccgttcg cggccagggc tatttgttcg aattgcgctg atgaaaaaat tactgcgtct
     6661 ttttttcccg ctctcactgc gggtacgttt tctgttggca acggctgcgg tagtactggt
     6721 gctttcgctt gcctacggaa tggtcgcgct gatcggttat agcgtcagtt tcgataaaac
     6781 tacgtttcgg ctgttacgtg gcgagagcaa tctgttctat acccttgcga agtgggaaaa
     6841 caataagttg catgtcgagt tacccgaaaa tatcgacaag caaagcccca ccatgacgct
     6901 aatttatgat gagaacgggc agcttttatg ggcgcaacgt gacgtgccct ggctgatgaa
     6961 gatgatccag cctgactggc tgaaatcgaa tggttttcat gaaattgaag cggatgttaa
     7021 cgataccagc ctcttgctga gtggagatca ttcgatacag caacagttgc aggaagtgcg
     7081 ggaagatgat gacgacgcgg agatgaccca ctcggtggca gtaaacgtct acccggcaac
     7141 atcgcggatg ccaaaattaa cctttgtggt ggtggatacc attccggtgg agctaaaaag
     7201 ttcctatatg gtctggagct ggtttatcta tgtgctctca gccaatctgc tgttagtgat
     7261 cccgctgctg tgggtcgccg cctggtggag tttacgcccc atcgaagccc tggcaaaaga
     7321 agtccgcgaa ctggaagaac ataaccgcga attgctcaat ccagccacaa cgcgagaact
     7381 gaccagtctg gtacgaaacc tgaaccgatt gttaaaaagt gaacgcgaac gttacgacaa
     7441 ataccgtacg acgctcaccg acctgaccca tagtctgaaa acgccactgg cggtgctgca
     7501 aagtacgctg cgttctctgc gtagtgaaaa gatgagcgtc agtgatgctg agccggtaat
     7561 gctggagcaa atcagccgca tttcacagca aattggctac tacctgcatc gtgccagtat
     7621 gcgcggcggg acattgctca gccgcgagct gcatccggtc gccccactgc tggacaatct
     7681 cacctcagcg ctgaacaaag tgtatcaacg caaaggggtc aatatctctc tcgatatttc
     7741 gccagagatc agctttgtcg gtgagcagaa cgattttgtc gaggtgatgg gcaatgtgct
     7801 ggataatgcc tgtaaatatt gcctcgagtt tgtcgaaatt tctgcaaggc aaaccgacga
     7861 gcatctctat attgtggtcg aggatgatgg acccggtatt ccattaagca agcgagaggt
     7921 cattttcgac cgtggtcaac gggttgatac tttacgccct gggcaaggtg tagggctggc
     7981 ggtagcccgc gaaatcaccg agcaatatga gggtaaaatc gtcgccggag agagcatgct
     8041 gggcggtgcg cggatggagg tgatttttgg tcgccagcat tctgcgccga aagatgaata
     8101 aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg cagataccag
     8161 cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt ttcttgaacg
     8221 tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta ttgacccgat
     8281 ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gtcgacagtc gactggtcag
     8341 tcaccaggat ggcaaatggc aggtcagcca cggtccgttc gaaagctacg atcatctcgg
     8401 tgaaactaac tggtcattgt tagtgcaagc agtaaatcac tggcatgagc cgaccgccgc
     8461 gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga tgatctcttt
     8521 ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt ttatcattca
     8581 gggtaccgga cgccgtcgct ggcgagtggg cgaaaagctg caaatgaaac agcactgccc
     8641 acacccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag agctggagcc
     8701 tggcgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc tggaaaatgc
     8761 gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta gcggatttgc
     8821 cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg atgttccacc
     8881 tcgcgctcat cctgcggacg ttctgccgca agaaatggat aaactgcgtg agatgatgct
     8941 cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta tatcccagtc
     9001 acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg aaatctacga
     9061 tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgagtat tgcgcattgg
     9121 cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg cactggatgc
     9181 actcgccagc aacattgcgc tgaccgcgga gaattttggc gatgcgctgg aagatccgtc
     9241 attcctcgcg atgctcgcgg cgctggtcaa tagcggatac tggttcttcg aagggtaagt
     9301 ttgggtttaa gccggatgcg gcatcacatg ccgcatccct tttcgcttac ttccgttgcg
     9361 ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct tccagagtca
     9421 caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg cacggtaatc
     9481 ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt tccggttcaa
     9541 tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc tcaaccactt
     9601 tctcgcgcat attgtagtaa ctgtcttcaa tcaccagttc aatgtagcaa tcaggatgta
     9661 accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct tcaaactgtt
     9721 tacggtcgaa atcacggatg atgtagtgca tatcggctcg gtcaacggtg cctttcatgc
     9781 tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca tccgccggaa
     9841 cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct ttcgccgtgc
     9901 ccggatgaac attgttaccg acaattttga tattgaccga tgcggcgttg aagttttcaa
     9961 actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca tcgaaggcat
    10021 caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag gcgacgcgaa
    10081 tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg atttctgcaa
    10141 taccggcttt gtcatcggca cctaacaagg ttttaccatc ggtggtaatc aacgtctgac
    10201 ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg ccgataccca
    10261 gcgcaatatc gccaccgcga tagctttcaa caatttgcgg attaacattt ttgccgctgc
    10321 aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg ccagggacgt
    10381 tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg ataagcccca
    10441 tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc gtgctgggaa
    10501 cctgtctcac ccctgctttt gattgggtat ccagagacac gtagttcaaa aatcgctcaa
    10561 gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga agcaagaaaa
    10621 gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat cagcattacc
    10681 ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc aagcggtttg
    10741 ccgtagaatt accgccctca ttaagagtca ccaaggtggt taaccacaaa ccccgcatcg
    10801 gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac aaccgagttc
    10861 gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta aagaggtcat
    10921 tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg gcccttctgg
    10981 ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg attccggacg
    11041 catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct atgtgaacac
    11101 tgttttccaa agctacgcac ttttccccca catgaccgtg ttcgaaaatg tggcctttgg
    11161 gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg aagccctgcg
    11221 gatggtgcaa ctggaaacct tcgctcaacg taaaccgcat cagctctctg gtggtcaaca
    11281 gcaacgcgtc gccattgctc gcgcggtggt taacaagcct cgtctgttgt tgctggatga
    11341 gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc tgaaagcgtt
    11401 acagcgtaag cttggtatta ccttcgtctt tgtcactcac gatcaggaag aagcgctcac
    11461 catgtcagac aggattgtgg tgatgcgcga gggtcgtatt gagcaagatg gtacaccgcg
    11521 cgaaatttac gaagagccga aaaacctgtt tgttgccggc ttcattggcg aaatcaatat
    11581 gtttaacgcc actgtcatcg aacgtctaga cgagcagcgc gtacgcgcca acgttgaagg
    11641 ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac tgcatgttct
    11701 gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg ctgaagggct
    11761 gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg ttgttgaact
    11821 ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgacc ctgactttga
    11881 ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg tcgtactggc
    11941 tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc ggttggcttg
    12001 tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg acccgcgacg
    12061 acgccagttt cgtcaaaatg gtctttacgc tggataacta cacgcgtctg ctcgatccgc
    12121 tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc ctcgcctgcc
    12181 tggtgctggg ctacccgttt gcctggtttc tggcgaagct accacacaag gtgcgtccgc
    12241 tgctgctgtt tctgctgatt gttcccttct ggaccaactc actgattcgc atctacgggc
    12301 tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg ctgggcgtta
    12361 tcgacacacc aattcgtatc atgttcacgc ccagtgcggt gattatcggt ctggtttaca
    12421 ttctgctacc gtttatggtg atgccgctgt actccagtat cgaaaaactg gataagccgt
    12481 tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc cgtatcatta
    12541 ttccactgac gatgccggga attattgccg gatgtctgct ggtgatgctg ccagcgatgg
    12601 gcctgttcta tgtatccgac ctgatgggcg gtgcgaaaaa cctgctgatc ggtaacgtca
    12661 tcaaggtcca gttcctcaat attcgtgact ggccgtttgg tgcagccacc agcattacgc
    12721 tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt ctgctgaata
    12781 aaaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga ccgctatcta
    12841 cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca gctcgcgctt
    12901 tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga acaacgacag
    12961 cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga catttgctac
    13021 gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgtg gtaagccgtt
    13081 cgttagcgga atgctgtttg tggtgatgat gtcaccagat atcgtgatgg cgatttctct
    13141 gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc tgttctcgca
    13201 tatcaccttc tgcctgccgt ttgtggtggt gacggtgtat tcgcgcctga aaggttttga
    13261 cgtgcggatg ctggaagcgg cgaaagatct cggtgccagt gaatttacca ttctgcgaaa
    13321 aatcattctg ccactggcaa tgcctgcggt ggcggcgggc tgggtgttaa gctttaccct
    13381 gtcgatggac gatgtggtag tttcttcgtt cgtcaccgga ccaagttatg aaattctgcc
    13441 gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg cgctggcaac
    13501 catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg ctcgtgataa
    13561 aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct gctcgcggcg
    13621 ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac gctgtatttc
    13681 tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa agaaaccggt
    13741 attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtatgccaa gctgaaaacg
    13801 tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga taaaatgcgt
    13861 aaagaaggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag caatctcgat
    13921 ccagacatgc tcaataagcc gtttgacccg aataacgatt actccattcc gtatatctgg
    13981 ggtgcgacgg cgattggtgt taacggtgat gcggtggatc cgaaatctgt caccagctgg
    14041 gccgatctgt ggaagcctga gtacaaaggc agcctgctgt tgaccgacga tgcccgtgaa
    14101 gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga tccgaaagag
    14161 attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtggcagc ctttaactcc
    14221 gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat ctggaacggt
    14281 tctgctttcg ttgcacgcca ggcgggtacg ccaattgacg tggtatggcc gaaagaaggc
    14341 ggcattttct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa agaaggcgcg
    14401 ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc cgaaactatc
    14461 ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gcccggaagt ggcgaacgat
    14521 aaaacgctct acccggatgc tgaaaccatt aaaaatggtg aatggcagaa tgacgttggc
    14581 gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg ttaattcagc
    14641 aagcgggcgg tggcattacc gcccgttttt tatttagcaa ggatgtggct aatgagtcag
    14701 gacagtaaag tgttatcccg cgcttttctc ggtatcggtc tgatattgct cgttatttct
    14761 gcaatcatct tttattacca ttttactttt accaaaagcg cggttcatac agaaggaatc
    14821 attgttgatg ccgtttggta caacaatcac tcaaacgacg tcgatgataa tggttcctgg
    14881 tatccagtgg ttgcctttcg ccctacgcca gattacacgc taattttcaa ttcgaatatc
    14941 ggtagtgatt tttatgaaga tagcgaaggt gacagagtca atgtctatta tccgccaggg
    15001 catccagaac aagccgaaat aaacaatccc tgggttaatt tttttaaatg gggctttgtt
    15061 ggcatagcag gcattatttt cggtagtgtc ggtctgataa tcacgctccc ctccaccaaa
    15121 aaatctcgca ggaaacggaa gtcccgccca tgaaaaaatt cattatactg ctcagtttac
    15181 tgatactcct gcctctggta gcaaccagta aaccattgat tccaataatg aagacacttt
    15241 ttacggatgt taccggtact gtaccggatg ctgaagagat cgcacgtaaa gccgagcttt
    15301 ttcgccaaca aacgggaatt gcaccgttta ttgtgatttt acccgacatt aataatgaag
    15361 ccagtctcag acaaaatggt aaagcgatgc tggcgcatgc gtcatcttca ttgagtaatg
    15421 taaaaggaag tgttctgtta ctatttacta cccgcgaacc acggttaatt atgatcacca
    15481 acggccaggt tgaaagtggt ctggacgata aacatctcgg ccttctgata gaaaatcaca
    15541 cgctggctta tttaaatgca gatctctggt attagggaat caataatgca ttggctgttc
    15601 tacaagcaca gatattaaaa caaccgacgc cgccactgac gtattatccg catccaggac
    15661 aacagcatga aaatgctccc cccggcagca ccaacacatt aggttttatc gcctgggctg
    15721 cgacgtttat cttattttcc tggatctttt gctacacgac tcgttttatt tatgcgttaa
    15781 aatttgcagt ggcgatgact attgccaata tgggttatca ggctttatgt ttgtatatcg
    15841 ataatagctt tgcgattacc agaataacgc cactctgggc agggctaatt ggtgtctgta
    15901 cgtttattgc cgcattgtta ttgacgagta aacgataaaa gaggccggaa aagtgcgcgt
    15961 gtattatgcc ggatggggcg cgaacgcctt atccggccca caaaacccgc aaattcaatt
    16021 aattgcgtcc ccttgtaggc ctgataagcg tagcgcatca ggcaatgctt cctgctttat
    16081 aatcccttca gcaacttttc aacaaactca ggcaccacct ggcttgccgg gccgtaatat
    16141 ttctcggcaa attcattacc gacctgactt ggttcgagat tcagttccac ggtgtgcgcg
    16201 ccatgcagtt tcgcttcgtg aacaaaccca gccgccggat aaacatgccc ggaagtacca
    16261 atggcaatga aaatatcagc catcgacaac gccatataaa tttcatccat gccgagtggc
    16321 atttcgccaa accaaacgac gtgcgggcgc aagggtgccg ggaactggca acaatggcat
    16381 ttatcttctg gcgtaacgtc tccggtccag tcgagaacct gaccactttg cgaacaacga
    16441 actttcagca attccccatg catatgaatg acattggtat tacctgcgcg ttcatgcagg
    16501 ttgtctatat tctgcgttac cagcaaaaag cgatcgccga gggcgtcttg cagtttagcc
    16561 agcgcaagat gcgcggcgtt aggctgaatt tctggctgct gcagctgtcg acgacgggcg
    16621 ttataaaacg cttgcaccag ttcaggatcg cgatcgaaac cttccggcgt tgccacatct
    16681 tcaacccgat gttcttccca caggccatct gcggcgcgaa aggtacgaat acctgattcc
    16741 gcagaaatcc ctgcccctgt cagtacgagt actcttggtt tttccattgc ttccggcacc
    16801 actttatctc tgaaaaaaat acgctgacgc aaacgctcgc gcaggcggcg tttattttta
    16861 cgaaaacgac ttaaccgatg accccgacgc gacagcatag caacctctgt tgtttaatcg
    16921 gttagatgta ggaaggccgc accacgcatt cctcccgcat caccgtggcg cgcgcgttca
    16981 atgcgcggaa cacgagctac aggtaagaga tgacgaggca gcctgtccgc cagttgcgtt
    17041 gtgattgccg ggaaattcga taagccacca ccaatgacga ccaggtcagg gtcaacaatg
    17101 gtcaggatat ttcccagaca aaccgctaat aaatccagat aacgctcaac gtgcgccctt
    17161 gcctgctcat cgccttgatc ataaagcgca ataatttcgg gagcctgcaa cggttgatga
    17221 taatagtgtt gatacagcca cgcaaaaccg cgaccagaca gataattttc aatgcagcca
    17281 tgctgaccac agccgcagcg gcgtaacggg aaatccaacc ccatcatggt taacgcatca
    17341 accggcagac gcatatggcc aaactcgccg gtaatgtagc ttttaccggt aatcggtttg
    17401 ccgttgaaaa tcagcccgcc gccaacgccg gtgccgagaa tcaaccccat caccagtgga
    17461 tattgagtaa attcatcatc ccaggcttct gaaagggcaa aacagttggc atcgttatca
    17521 aggcgtacat cgcgatcaag acgtgcgctc aggtcggcac gcagcggttt accgctggca
    17581 gcggggacat tggcggcata cagcgtacca tcttctgttt ccggcattcc cggaataccg
    17641 atgccgacag aacctttaca gccaaaacgt tggtcagctt cagctaccag ctcacacact
    17701 gcatctaaaa atgcgtcata gctgtcacgc ggtgtcggca cccgcttttc ccactgcaac
    17761 tgccgaccgc tatcaaacac gccaagcgca atttttgttc caccaatatc aaacccgtaa
    17821 tacattgccg ctccttgttt taatgtactg cctttactgg ccgctaagga ctcgcgcagg
    17881 gtcaatatta ctggcgcgcc gcgccggata ccaacttgcc aaaagactca gcaacaatgc
    17941 tgtgaccagt acgtagaaga cgtccagcca gtgcaattcc gatggcagga aatcaataaa
    18001 atagatatcg ctggagagga actgatgacc gatcaacttt tcaatccact caataatcgg
    18061 ggtaagttgc agtgaaacca ccacaccgat aatcacccca cacaggctgc cgaacagccc
    18121 tgccagcaat ccataccaga caaagatggc gcgaattaaa ccatctttcg cccccagcgt
    18181 tcttaatact gcgatatcgc cactcttgtc tttcaccgcc atcactaagg tggagacgat
    18241 gttgaaacag gccacgccaa tcaccagtac catcgccaga tacataatgg cgcggatcat
    18301 ctggatatcg cgatacatat agccgtaagt accaatccaa cttttaatat aaacatagct
    18361 gttggtcact tcacccgcat cgcgtaccag cttattggcg ttgaaaacat ccgtcatttt
    18421 aagggcaata cctgacacgc tggaacccat atcaagatat tgttgggcat ccgccagcgg
    18481 gatcatggca aaactgtgat cgagttgacc actcaactgc aaaataccgg caacgtgcaa
    18541 acgcacacgt tttggctgca tcagtttatg ctcgggattc gagttgggga tcataatcga
    18601 cacccaatcc ccctgcttca ctttcagcgc atccgccacg cctttgccga tgataatttg
    18661 ctgttcgccc gctttaaaat tgcgccacgc atcgccctgg acaaacgagg gtaatgcgct
    18721 cagacgctgt tcctgttgcg ggttaacgcc cttcacctgg attgcgcgaa gattcacgcc
    18781 actttccacc agcccggtga aattgatata cggcgcggca gcggcaatac ccggcacttt
    18841 ttgcacgtga tccagtgctt cctgccagtt agtccacggc tgatccaccg cctcgatttc
    18901 accatgcggc accaccgcca gaatacggtt gttcagttcg cgttcaaagc cgttcatcgc
    18961 gcttaagccg acgatcaata ccgccacgcc gagggcaatg ccaatggtag aaataacgga
    19021 gatcagcgac accatgccgc cgcgccgccg tccgcgacta aaacgcaggc caattaataa
    19081 cgataaaggc atcgccatta ctccgccccc atcaggctca gttccgccgt cagacgacca
    19141 tcacgcattt ctaactggcg gctcatacgt ttcgccagtt gcaggtcgtg agtaaccacc
    19201 aggaaggcgg tgccctgcaa gcgattcaat tccccaagca actggaagat gctgtcggcg
    19261 ttacgcgcat cgaggttacc ggtaggttca tccgccagta ccaggcgcgg gttattcacc
    19321 agcgcacggg caatcgccac acgctggcgt tcgccgccag aaagttcaga tgggcggtga
    19381 ttcgcacgat gatccagccc caccgctttt aacatctcaa gtgcacggct gttgatttca
    19441 gcgggctttt tcttgccaat cagcagtggc atagccacgt tttccagggc agtaaaatcc
    19501 ggcagcaggt ggtgaaactg ataaataaag cccagcttct ggttgcgcag ttctgctttc
    19561 gccgccgaag acagtttgct catcggttga ccgttaaaga tcacatcgcc ggaggttggt
    19621 gtatccagcc cgcccagcag gtgcagcaag gtacttttac cggaaccaga gctaccgacg
    19681 atcgccatca tttcaccttc gcccacgctg aaactgacat tgtgcagtac atcggtttgc
    19741 acactgcctt cctgatagcg tttgcacagg ttgtcgcatt gcaacaggat cttattcata
    19801 acgtaaagcc tcagcgggtt gtgtggcggc agcgcgccat gaagggtaaa gcgtagacag
    19861 cagcgcgata gccatcgcca ccagcgcaat aacaatgacc tgtaaaggtt cgatagccac
    19921 cggcagcgcc gcgccatcaa gcaggacgcc gattatcggc atcagattat ttaactggct
    19981 ggcaagcagt gcgccaagcg ccgcaccgag gatcgcaccg ataatcccgg cgctggcccc
    20041 ttgcaccata aagaccatca tgatttgtcg cggagttaag ccttgcgttt gcaggatcgc
    20101 gacttcgccc tgcttctcca ttaccatcag ccctagtgag gtaataatgt taaacgccgc
    20161 aacggcgaca atcaggctca gcagcaagcc catcatattt ttttccatgc gtacggcctg
    20221 gaacagctcg cctttacggt cacgccagtc ctgccatttg ctgccttcag gcagtttttg
    20281 ctgacttaaa gagtcaactt tcagcggctc atccagccac aaacgccagc cggtaatatt
    20341 gcctgccgga taacgcatca gacgcgaggc atcctcaata ttcaccagca tttcatagcc
    20401 atcgacttca ctgttggcgg cgaaagtgcc aatcacattg aacaggcgct ggcttggaat
    20461 acgccccatc ggcgtgaact ggctggcaga tggtaccatc acgcggattt gatcaccgcg
    20521 attaacgcct agctgtgagg caagttgttc gccgaggatg acattatatt tccccggctc
    20581 gaggtcagtt tgtttcacat tgaccagata cggtgtaagt ggatcttttt gcgccgggtc
    20641 gataccgagc atcaccccga ccgccacgct gcgcgcgctt tgcagtacca catcaccggt
    20701 agtaataggt gcgacgcgat taacgccgtc cagtttgact gccgtttccg ggagttgctg
    20761 cgggttaaga gagccatgct cagaagagag aattgcctgt ggcatcaggc caaggatgtt
    20821 gttttgcagc tcgcgctcaa agccgttcat cactgacaat actgtgacca gcgccatcac
    20881 cccgagggta atgccgatgg tagaaagcca ggagacgaaa cgaccgaagc gatccgctgc
    20941 acgcccacgc atgtaacgca ggccaataaa tagagcgaca ggttggtaca tgaaatccgt
    21001 ctggttgctg tagcaaagac acgagtatat aagcgaaagc aagatgggta aacgtctgaa
    21061 ctgtaagtga tcgtcagggc taatctgaaa agcccccgta aatcaatcgg ctaaattgcc
    21121 atgttttatc atcaatcttg ccgcctgatc ttaatctgaa aaatcatcca atacagacag
    21181 atacccatta cacctcgggt cataacccgc aggatcaacc gcatgtaaat taaaaaagag
    21241 cgtgttgtcc tgatgaaaca aaaagagcta tggattaacc agatcaaagg gttatgtatt
    21301 tgtctggtgg tgatttatca ctcggtcatt accttttatc cgcatctgac cacttttcag
    21361 catccgttat cggaagtcct gagcaaatgc tggatctatt tcaatcttta ccttgccccc
    21421 tttcgtatgc cggttttttt ctttatatcc ggctatttga ttcgccgcta tatcgacagc
    21481 gtaccgtggg gaaattgtct cgatatacgg atctggaaca tcttctgggt gctggcgctt
    21541 tggggcgtgg tgcagtggct ggcgttaagt gcgctgaacc agtggctggc acctgagcgc
    21601 gatttaagca atgcctccaa tgccgcttat gccgattcca ccggtgagtt cctgcacggg
    21661 atgatcaccg ccagcaccag cttgtggtat ctgtatgcgt taattgtcta tttcgtggta
    21721 tgtaaaattt ttagccgtct ggcgctgcca ctattcgcct tgtttgtact gctgagtgtg
    21781 gcggttaatt tcgttcccac gccgtggtgg ggaatgaaca gtgtgatccg caatttgctt
    21841 tattacagcc ttggcgcatg gtttggcgca acaataatga cctgtgttaa agaggtgccg
    21901 ttgcgccgcc atctgctgat ggcttctttg cttaccgttc tggcggtcgg tgcctggttg
    21961 tttactatct cgctgctgtt gtcgctggta tcgattgtgg tgatcatgaa gctgttttat
    22021 caatacgagc aacgattcgg tatgcgctcc accagcctgc tgaatgtgat tggctccaac
    22081 accattgcta tctacaccac ccatcgcatt ctggttgaaa tattcagctt aactctgctt
    22141 gcgcaaatga acgcagcacg ctggtcgccg caagtcgaac tgacactcct gctggtttat
    22201 ccctttgtca gtttgttcat ctgtactgtt gcgggcttgc tggtaagaaa actttcacag
    22261 cgtgcattca gcgatctgtt gttctccccg ccttcactgc ccgctgccgt cagttactct
    22321 cgttaaacca tcgccccagc cggggcgatt caatttgcta aaccatgtcg attacggata
    22381 atgaacagca ttgcttatca gagatatgcc cccatatgtt gaggcatatc ctaacgagaa
    22441 tctgacaacc gttatgcctg aacaatatcg ttatacgctg cccgtcaaag cgggtgagca
    22501 gcgtctgctg ggcgagttaa ccggcgcagc ctgtgcaacg ctggtagcgg aaattgccga
    22561 acgtcacgcc ggtccggtgg tactcattgc accagatatg caaaatgctc tgcgtttgca
    22621 tgatgaaatc agccagttca ccgatcagat ggtgatgaat ctggcggact gggaaactct
    22681 tccctacgac agtttttcgc ctcatcagga cattatctcc tcgcgccttt ccacccttta
    22741 ccagctaccg acgatgcagc gtggcgtact gattgttccg gtgaatacgc ttatgcagcg
    22801 cgtttgccca cacagttttc tccacggtca tgcgctggtg atgaaaaaag gtcagcgcct
    22861 gtcacgagat gcattacgaa cccaactgga cagcgccggt tatcgccatg ttgaccaggt
    22921 gatggagcac ggcgaatacg ccacgcgcgg cgcgttgctg gatctcttcc cgatggggag
    22981 tgagctgcct tatcgtcttg atttctttga tgatgaaatc gacagcctgc gggtgtttga
    23041 cgtcgacagc cagcgcacgc tggaggaagt agaagcgatc aatctgctgc ccgcgcacga
    23101 atttccgacc gataaagcgg caattgaact gttccgcagc cagtggcgcg ataccttcga
    23161 agtgaagcgc gatccggaac atatttacca gcaagtgagt aaaggcacat tacctgccgg
    23221 gatcgagtac tggcagccat tgttcttcag cgaaccactg ccgccgctgt tcagttattt
    23281 ccctgccaat accttgctgg tgaatactgg cgatctggaa accagtgccg aacgtttcca
    23341 ggctgacacg ctggcgcgtt ttgagaatcg cggcgtcgat ccgatgcgcc cgctgttgcc
    23401 accacaatcg ctctggctgc gggtggacga gctcttctca gagctgaaaa actggccccg
    23461 ggtgcagcta aaaactgaac atttaccgac aaaagccgcg aataccaatt taggtttcca
    23521 gaaactgcca gacctggccg ttcaggcaca acaaaaagcg ccgctggatg cgctgcgtaa
    23581 gttcctcgag actttcgacg gtccggtggt gttctcggta gaaagtgaag gtcgccgtga
    23641 agcgctgggt gaactgctcg cacgaattaa aattgctccg caacgcatta tgcgtcttga
    23701 tgaagccagc gaccgtgggc gttatctgat gattggcgct gccgaacatg gttttgtcga
    23761 taaggtgcgt aatctggcgc tgatctgcga aagcgatctg ctcggtgaac gtgttgcccg
    23821 ccgtcgtcag gattctcgcc gcaccatcaa ccccgataca ctgatccgta accttgcgga
    23881 gctgcatatt ggtcagccgg tggtccatct ggagcacggt gtcgggcgct atgccggaat
    23941 gaccacgctc gaagcgggcg gcattactgg cgagtatttg atgctcacct atgccaacga
    24001 cgccaaactg tatgttccgg tgtcgtcact gcatctgatt agccgttacg caggtggcgc
    24061 ggaagaaaac gccccgctgc ataaacttgg cggcgatgcg tggtcacgcg cgcggcagaa
    24121 agcggcggaa aaagtgcgtg atgtggcggc ggaattgctg gatatctacg cgcaacgcgc
    24181 cgccaaagag ggcttcgcgt ttaaacacga tcgtgagcag tatcagttgt tctgcgacag
    24241 cttcccgttt gaaaccacgc cggatcaggc gcaggccatt aatgcggtac ttagcgacat
    24301 gtgtcagccg ctggcaatgg atcgtctggt gtgcggcgat gttggctttg gtaaaacaga
    24361 agtggcgatg cgcgccgctt tcctggcagt agataaccac aagcaggtgg cggtgttggt
    24421 gcctaccacc cttctcgcgc agcagcatta cgacaacttc cgcgaccgtt tcgccaactg
    24481 gccggtacgt atcgaaatga tctcccgttt ccgcagcgcc aaagagcaga cgcaaatcct
    24541 tgcggaagtg gcggaaggga aaatcgatat tctgatcggt acgcacaaac tgctgcaaag
    24601 tgacgtcaag tttaaagatt taggcctgct gattgtcgat gaagaacacc gcttcggggt
    24661 gcgtcataaa gagcgtatta aagcgatgcg cgcgaacgtg gatattctga cgcttactgc
    24721 aacgccgatc ccacgtacgc tgaatatggc aatgagcgga atgcgtgacc tgtcgattat
    24781 cgccacgccg cccgcccgtc gtctggcagt taaaaccttt gtccgtgagt atgacagcct
    24841 ggtggtccgg gaggcgatcc tgcgtgaaat tttgcgcgga ggacaggttt attatctcta
    24901 caatgatgtg gaaaacattc agaaagccgc cgaacggctg gcagaactgg tgccagaagc
    24961 gcggatcgcc atcggtcacg ggcagatgcg cgagcgcgaa ctggaacggg tgatgaatga
    25021 tttccatcat caacgtttca acgtgctggt ttgtaccacc attatcgaaa ccgggatcga
    25081 catcccgaca gccaacacca ttatcattga acgtgcggat cacttcggtc tggcgcagct
    25141 gcaccagtta cgcggtcgcg tcggacgttc gcatcatcag gcatatgcat ggttgctgac
    25201 gccgcatcca aaagcgatga ctaccgatgc acaaaaacgt cttgaagcga ttgcctcgct
    25261 ggaagatctc ggtgcaggct ttgcgctggc aacgcacgat ctggagattc gcggcgcggg
    25321 tgaactgctt ggcgaagaac aaagcggctc aatggaaacc atcggtttct cgctgtatat
    25381 ggagttgctg gaaaacgccg tcgatgcact gaaagccgga cgcgagccgt cgctggaaga
    25441 tctcaccagc cagcaaacag aagtcgagct gcggatgccg tcgctattgc cagatgattt
    25501 cattcctgac gtgaatacgc gtttgtcgtt ctataaacgt attgccagcg ccaaaacgga
    25561 aaacgaactg gaagagatca aagtcgagct tatcgatcgc ttcggcctgc tgccggatcc
    25621 ggcgcgtatc ctgctggatg ttgcccgtct gcgccagcaa gcgcagaaac tggggatcag
    25681 gaagctggaa ggtaatgaga aaggcggggt gatcgaattt gccgagaaga atcacgttaa
    25741 tccggcctgg ttgattggtt tgctgcaaaa acagccgcag cattaccgcc ttgatggtcc
    25801 gacgcgcctg aaatttattc aggatttgag tgagcggaaa acgcgtatcg aatgggtacg
    25861 ccagtttatg cgtgaactgg aagagaacgc gatcgcttaa tggtaaatcg ggggcgtttc
    25921 tgcgccccca tctgcaacat ttacaaattc tttgcacttc cctgcactat ccgacactgt
    25981 caccatccat aattcaggct tatctgttta ttacaataac cttatattta ttatggattt
    26041 ttggtgatga tcaaaacgcg tttttctcgc tggctaacgt tttttacgtt cgccgctgcc
    26101 gtggcgctgg cgctaccggc aaaagccaac acctggccgc tgccgccagc gggcagtcgt
    26161 ctggttggcg aaaacaaatt tcatgtggtg gaaaatgacg gtggttctct ggaagccatc
    26221 gccaaaaaat ataacgtcgg ctttctcgct ctgttacagg ctaaccccgg cgttgatcct
    26281 tacgtaccgc gcgcggacag cgtgttaacg atcccgttgc aaaccctact tccagacgcg
    26341 ccgcgcgaag gcattgtgat caacattgcg gagctgcgtc tctattacta cccgccgggt
    26401 aaaaattcgg taaccgtgta tccaataggt attggtcagt taggtggtga cacgctgaca
    26461 ccgacaatgg tgaccaccgt ttcagacaaa cgtgcaaacc caacctggac gccaacggca
    26521 aacatccgcg cccgttataa agcacaggga attgagttgc ctgcggtagt gccggctgga
    26581 ccggataacc caatgggcca tcatgcgatt cgtctggcgg cctatggcgg cgtttatttg
    26641 cttcacggta cgaacgccga tttcggcatt ggcatgcggg taagttctgg ctgtattcgt
    26701 ctgcgggatg acgatatcaa aacactcttt agccaggtca ccccaggcac caaagtgaat
    26761 atcatcaaca ctccgataaa agtctctgcc gaaccaaacg gtgcgcgtct ggttgaagta
    26821 catcagccgc tgtcagagaa gattgatgac gatccgcagc tgctgccaat tacgctgaat
    26881 agcgcaatgc aatcatttaa agatgcagca caaactgacg cagaagtgat gcaacatgtg
    26941 atggatgtcc gttccgggat gccggtggat gtccgccgtc atcaagtgag cccacaaact
    27001 ctgtaaatcc tggataaaaa aaatccccgc gacacggcat gtcgcgggga aaaaatcagc
    27061 agtagcaggc attaatgagg gttaatgctt atttataaat tactgctgtt ccatggaggg
    27121 tattcggacc ggttacagaa gtaatacgga aagattttgc gcccatctca tccgcttttt
    27181 gcgccagctg ctcttccagc gatcccagat ttgtccccgc gttagcactg attgtaccga
    27241 ctttttgttg gccttctggc gttgactgaa cttcgacagc cgcaaagctg gcaaatgaca
    27301 tggagcttaa aatcgccgca gcgatgaggg tttttacgtt tttcataata gtggccttat
    27361 gcagatgaat gacgtcgcaa gcaattactt aacgatcgat aactaaatga ta
//
LOCUS       DAPHDW010000053        27253 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.053, whole genome shotgun sequence.
ACCESSION   DAPHDW010000053 DAPHDW010000000
VERSION     DAPHDW010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 27253)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27253)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27253
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.053"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(124..687)
                     /gene="fimA"
                     /locus_tag="RH057_002229"
     CDS             complement(124..687)
                     /gene="fimA"
                     /locus_tag="RH057_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV3128591.1"
                     /translation="MKLKHVGMIVVSVLAMSSAAVSAAEGDESVTTTVNGGVIHFKGE
                     VVNAACAIDSESMNQTVELGQVRSSRLAKAGDLSSAVGFNIKLNDCDTNVSSNAAVAF
                     LGTTVTSNDDTLALQSSAAGSAQNVGIQILDRTGEVLILDGATFSAKTDLIDGTNILP
                     FQARYIALGQSVAGTANADATFKVQYL"
     gene            complement(1119..1327)
                     /locus_tag="RH057_002230"
                     /pseudo
     CDS             complement(1119..1327)
                     /locus_tag="RH057_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226947.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(1639..2961)
                     /gene="hipA"
                     /locus_tag="RH057_002231"
     CDS             complement(1639..2961)
                     /gene="hipA"
                     /locus_tag="RH057_002231"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system serine/threonine
                     protein kinase toxin HipA"
                     /protein_id="HDV3128592.1"
                     /translation="MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLASRYARPLSLS
                     LPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYHAKSRQPFDLLSEIGRDSVGAVT
                     LLPENETITRPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
                     LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVP
                     DAEIIKAGNVRALAVERFDRRWNAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIA
                     RIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQAGGSYRLTPFYDII
                     SAFPVLGGTGIHISDLKLAMGLNASKGKKTAIDKIYPRHFLATAKVLRFPEVQMHEIL
                     SDFARMIPAALDNVKTSLPTDFPENVVTAVESNVLRLHGRLSREYGSK"
     gene            complement(2961..3227)
                     /gene="hipB"
                     /locus_tag="RH057_002232"
     CDS             complement(2961..3227)
                     /gene="hipB"
                     /locus_tag="RH057_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HipB"
                     /protein_id="HDV3128593.1"
                     /translation="MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISN
                     FENTPDNTTLTTFFKILQSLELSMTLCDTKNASPESTEQQDLEW"
     gene            complement(3436..4836)
                     /locus_tag="RH057_002233"
     CDS             complement(3436..4836)
                     /locus_tag="RH057_002233"
                     /inference="COORDINATES: protein motif:HMM:TIGR01414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3128594.1"
                     /translation="MNSEGGKPGNVLTVNGNYTGNNGLMTFNATLGGDNSPTDKMNVK
                     GDTQGNTRVRVDNIGGVGAQTVNGIELIEVGGNSAGNFALTTGTVEAGAYVYTLAKGK
                     GNDEKNWYLTSKWDGVTPADTPDPINNPPVVDPEGPSVYRPEAGSYISNIAAANSLFS
                     HRLHDRLGEPQYTDSLHSQGSASSMWMRHVGGHERSRAGDGQLNTQANRYVLQLGGDL
                     AQWSSNAQDRWHLGVMAGYANQHSNTQSNRVGYKSDGRISGYSAGLYATWYQNDANKT
                     GAYVDSWALYNWFDNSVSSDNRSADDYDSRGVTASVEGGYTFEAGTFSGSEGTLNTWY
                     VQPQAQITWMGVKDSDHTRKDGTRIETEGDGNVQTRLGVKTYLNSHHQRDDGKQREFQ
                     PYIEANWINNSKVYAVKMNGQTVGREGARNLGEVRTGVEAKVNNNLSLWGNVGVQLGD
                     KGYSDTQGMLGVKYSW"
     gene            complement(4833..8850)
                     /locus_tag="RH057_002234"
                     /pseudo
     CDS             complement(4833..8850)
                     /locus_tag="RH057_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310144.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter barrel domain-containing
                     lipoprotein"
     gene            complement(9381..10973)
                     /gene="lsrK"
                     /locus_tag="RH057_002235"
     CDS             complement(9381..10973)
                     /gene="lsrK"
                     /locus_tag="RH057_002235"
                     /EC_number="2.7.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416028.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer-2 kinase"
                     /protein_id="HDV3128595.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIAPEYIAAVSACSMREGIVLYNNE
                     GAPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRDWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTPVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     WQQVVNLAAPVTDPEMNVRVNPHVIPGMVQAESISFFTGLTMRWFRDAFCAEEKLIAE
                     RLGIDTYTLLEEMASRVPPGSWGVMPIFSDRMRFKTWYHAAPSFINLSIDPDKCNKAT
                     LFRALEENAAIVSACNLQQIADFSNIHPSSLVFAGGGSKGKLWSQILADVSGLPVNIP
                     VVKEATALGCAIAAGVGAGIFSSMAETGERLVRWERTHTPDPEKHELYQDSRDKWQAV
                     YQDQLGLVDHGLTTSLWKAPGL"
     gene            complement(11052..12005)
                     /gene="lsrR"
                     /locus_tag="RH057_002236"
     CDS             complement(11052..12005)
                     /gene="lsrR"
                     /locus_tag="RH057_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HDV3128596.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSSLKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            12254..13789
                     /gene="lsrA"
                     /locus_tag="RH057_002237"
     CDS             12254..13789
                     /gene="lsrA"
                     /locus_tag="RH057_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HDV3128597.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGMAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFTRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPVVREKSLSASQKLWLELPGNRPQHAAGTPVLTLENLTG
                     EGFRNVSMTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGGNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLLISSDLEEIELMADRVYVMHQGEIAHSALTGRDINVETIMRVAFGDSQRQE
                     VSC"
     gene            13783..14811
                     /gene="lsrC"
                     /locus_tag="RH057_002238"
     CDS             13783..14811
                     /gene="lsrC"
                     /locus_tag="RH057_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HDV3128598.1"
                     /translation="MLKFIQNNREITALLAVVLLFALPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGVLVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLFGVS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFIPNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            14811..15803
                     /gene="lsrD"
                     /locus_tag="RH057_002239"
     CDS             14811..15803
                     /gene="lsrD"
                     /locus_tag="RH057_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HDV3128599.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            15815..16837
                     /gene="lsrB"
                     /locus_tag="RH057_002240"
     CDS             15815..16837
                     /gene="lsrB"
                     /locus_tag="RH057_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
                     /protein_id="HDV3128600.1"
                     /translation="MTLHRFKKIALLSALGIAAISMNVQAAERIAFIPKLVGVGFFTS
                     GGNGAQQAGKELSVDVTYDGPTEPSVSGQVQLINNFVNQGYNAIIVSAVSPDGLCPAL
                     KRAMQRGVRVLTWDSDTKPECRSYYINQGTPAQLGGILVDMAARQVNKDKAKVAFFYS
                     SPTVTDQNQWVKEAKAKIAKEHPGWEIVTTQFGYNDATKSLQTAEGILKAYSDLDAII
                     APDANALPAAAQAAENLKNDKVAIVGFSTPNVMRPYVERGTVKEFGLWDVVQQGKISV
                     YVADALLKKGSMKTGDKLDIQGVGQVEVSPNSVQGYDYEADGNGIVLLPERVIFNKEN
                     IGKYDF"
     gene            16864..17739
                     /gene="lsrF"
                     /locus_tag="RH057_002241"
     CDS             16864..17739
                     /gene="lsrF"
                     /locus_tag="RH057_002241"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HDV3128601.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGALDWGMQSRLSRIF
                     NPKTGNTVMLAFDHGYFQGPTTGLERIDINIAPLFEHADVLMCTRGILRSVVPPATNK
                     PVVLRASGANSILAELSNEAVALSMDDAVRLNSCAVAAQVYIGSEYEHQSIKNIIQLV
                     DAGMKVGMPTMAVTGVGKDMVRDQRYFSLATRIAAEMGAQIIKTYYVEKGFERIVAGC
                     PVPIVIAGGKKLPEREALEMCWQAIDQGASGVDMGRNIFQSDHPVAMMKAVQAVVHHN
                     ETADRAYELYLSEKQ"
     gene            17763..18053
                     /gene="lsrG"
                     /locus_tag="RH057_002242"
     CDS             17763..18053
                     /gene="lsrG"
                     /locus_tag="RH057_002242"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HDV3128602.1"
                     /translation="MHVTLVEINVHEDKIDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDAVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            18110..18868
                     /gene="tam"
                     /locus_tag="RH057_002243"
     CDS             18110..18868
                     /gene="tam"
                     /locus_tag="RH057_002243"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HDV3128603.1"
                     /translation="MSDWNPSLYLHFAAERSRPAVELLARVPLENIKYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLIESEQQLFL
                     KRYHQMLEEQYPLQENGQILLAFPRLFIVARRME"
     gene            complement(18872..19786)
                     /locus_tag="RH057_002244"
     CDS             complement(18872..19786)
                     /locus_tag="RH057_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bestrophin family protein"
                     /protein_id="HDV3128604.1"
                     /translation="MIVRPQQHWLRRIFVWHGSVLSKISSRLLLNFLFSIAVIFMLPW
                     YTHLGIKFTLAPFSILGVAIAIFLGFRNNAGYARYVEARKLWGQLMIASRSLLREVKT
                     TLPDSASVREFARLQIAFAHCLRMTLRKQPQAEVLAHYLKTEDLQPVLASNSPANRIL
                     LIMGEWLAVQRRNGQLSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTV
                     YLFCIMLPFALVVDLHYMTPFISVLISYTFISLDCLAEELEDPFGTENNDLPLDAICN
                     AIEIDLLQMNDEAEIPAKILPDRHYQLT"
     gene            complement(19993..21444)
                     /gene="uxaB"
                     /locus_tag="RH057_002245"
     CDS             complement(19993..21444)
                     /gene="uxaB"
                     /locus_tag="RH057_002245"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HDV3128605.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKAKGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIVLAEKDHWE
                     QDLTQVPGLVEQVANDLDAILEKGMREAVRPLC"
     gene            complement(21671..23089)
                     /locus_tag="RH057_002246"
     CDS             complement(21671..23089)
                     /locus_tag="RH057_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3128606.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIALIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINSLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(23228..23587)
                     /locus_tag="RH057_002247"
     CDS             complement(23228..23587)
                     /locus_tag="RH057_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HDV3128607.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVVQMNQP"
     gene            complement(23587..24513)
                     /gene="glsB"
                     /locus_tag="RH057_002248"
     CDS             complement(23587..24513)
                     /gene="glsB"
                     /locus_tag="RH057_002248"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HDV3128608.1"
                     /translation="MAVAMDNAILGNILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINAGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(24577..25965)
                     /gene="sad"
                     /locus_tag="RH057_002249"
     CDS             complement(24577..25965)
                     /gene="sad"
                     /locus_tag="RH057_002249"
                     /EC_number="1.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate-semialdehyde dehydrogenase"
                     /protein_id="HDV3128609.1"
                     /translation="MTITPATHAISINPATGEQLSVLPWAGANDIENALQLAAAGFRD
                     WRETNIDYRAEKLRGIGKALRARSEEMAQMITREMGKPINQARAEVAKSANLCDWYAE
                     HGPAMLKAEPTLVENQQAVIEYRPLGTILAIMPWNFPLWQVMRGAVPIILAGNGYLLK
                     HAPNVMGCAQLIAQVFKDAGIPQGVYGWLNADNDGVSQMIKDSRIAAVTVTGSVRAGA
                     AIGAQAGAALKKCVLELGGSDPFIVLNDADLELAVKAAVAGRYQNTGQVCAAAKRFII
                     EEGIASAFTERFVAAAAALKMGDPRDEENALGPMARFDLRDELHHQVEKTLAQGARLL
                     LGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGPVAAITVAKDAEHALELANDSEFG
                     LSATIFTTDETQARQMAARLECGGVFINGYCASDARVAFGGVKKSGFGRELSHFGLHE
                     FCNIQTVWKDRI"
     gene            26066..26947
                     /locus_tag="RH057_002250"
     CDS             26066..26947
                     /locus_tag="RH057_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3128610.1"
                     /translation="MDLTQLEMFNAVAEAGSITQAAAKVHRVPSNLTTRLRQLETELG
                     VDLFIRENQRLRLSPAGHNFLRYSQQILALVDEARSVVAGDEPQGLFSLGSLESTAAV
                     RIPATLAEFNHRYPKIQFSLSTGPSGTMLEGVLEGKLNAAFIDGPINHTAIDGIPVYR
                     EELMIVTPQGHAPVIRASQVNGSNIYAFRANCSYRRHFESWFHADGAAPGTIHEMESY
                     HGMLACVVAGAGIALIPRSMLESMPGHHQVEAWPLAEQWRWLTTWLVWRRGAKTRPLE
                     AFIQLLDVPDSAKQGYQ"
     gene            27025..>27253
                     /gene="yneK"
                     /locus_tag="RH057_002251"
     CDS             27025..>27253
                     /gene="yneK"
                     /locus_tag="RH057_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HDV3128611.1"
                     /translation="MVTPVSISNYISLPDDFPVRNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNINSATKKIFNNLDNLSYIQRTSFRG"
BASE COUNT         6840 a         7041 c         6427 g         6945 t
ORIGIN      
        1 ccaaatacaa accatttctc attaaaaatg agatgaatat tggtaaattg ctcgggtgtg
       61 cgtagccatg ccacacacac cctgtttcaa tttcagatga cgtatagcca ccttaagatt
      121 gaattataga tattgaactt tgaaggtcgc atcagcgttt gcagtaccag ctacggactg
      181 cccgagagca atataacgag cctggaatgg cagtatattc gtgccatcaa tcaagtcggt
      241 tttagcacta aaagtggccc catcaagtat taatacctca cccgtacggt ccaaaatttg
      301 aataccgaca ttttgggcag agcctgccgc tgaactctgc agcgctaacg tatcgtcatt
      361 actggtgaca gtagtaccca ggaatgcaac agctgcatta ctggaaacat tggtatcaca
      421 atcattcagc ttgatattga agccaacggc ggagctgagg tcacccgctt tagccaggcg
      481 agaagaacga acctgaccca gctcaaccgt ttggttcatt gattctgaat caattgcaca
      541 agcggcattt accacttcac ctttaaaatg aataacaccg ccattaacag tggtcgttac
      601 tgattcatca ccctcggctg cgcttaccgc agcagacgac atcgccaaca cagaaacgac
      661 aatcatacca acatgtttga gtttcataat tctccaaaat ttatatttaa gtagctgaat
      721 aaagagagtt aacaattaag tcattctgtt attgcttaaa aacaagaatc attcttgatt
      781 ggttgttgcc ctgagaatat agtttccagg gttattacat ttcacgaaga acgataatat
      841 ctttaaatca caaggacacc taatcacaac aagtttattg tgcacttgtt gaagagagta
      901 tttgcaaaaa cattttatgt cgctaaataa catgacgatt tcattatgca tgttatttat
      961 aaaccgtgac taaacagaat attattgcga acaagggaag tgtcaggaaa ggtcaagggc
     1021 tactaatggt taatatggct ataaatcatt agctatggtt aatgtttgct tgctgaaact
     1081 aacaccgaat tgctctaaac agataagtgc agggtttatt aaagctgacc gacagacttt
     1141 cttaacctgg taatatctct cttgggtgac tctccaaaca tccgcgaata ttcccaactg
     1201 aaatgggata gctttcgtaa ccgacagcat aggctgctgt ggtgacatcg taatgttcat
     1261 tcagcatcgg tcgcctgact tcattgagac gtagccattt ctgataccgg agtggactca
     1321 ttcccgcttg atccgtcctt aaataagtaa cagcaccctc tggtgttttc gtatgcagtg
     1381 tctatgcgac agacactgcg ctcttagcat ggggcaggga ataaccaggc tatttctcca
     1441 tgcacaggcc aattatgaaa gaggagaggc ttaagttctt gaaccctgaa cgaataacga
     1501 ctaccagaaa agcacttcgc atcactcaga catgatttaa cataatatac attatgcgca
     1561 ccagtataaa ccaaggaaga atccagtcgt tggcggtcat gattgtcatg ctcattaaca
     1621 atgaccaaac cccatatctc acttactacc gtattctcgg cttaaccgtc catgcaacct
     1681 caacacattg ctttcaactg ccgtcaccac gttctccgga aaatctgtcg gtaatgaagt
     1741 cttcacgtta tccagtgctg ctggaatcat tctggcaaag tcactcagga tttcatgcat
     1801 ctgcacttcc gggaatctca gcacctttgc tgtcgccaaa aaatgtcgcg gataaatttt
     1861 atcgattgcc gtttttttgc ctttggatgc gttaagcccc attgccagtt tgagatcgct
     1921 gatgtgtatt cccgtaccgc caaggaccgg aaatgctgaa atgatgtcgt aaaatggcgt
     1981 gagtcgataa ctgccgccag cctgaataaa tacggagaag ttttttgcat gaccgtccgt
     2041 tgcgccaatc aaccactgga agacctggaa tttcataaaa tcatagcgat ctttcagcgc
     2101 ctcgctggac cccatcaaaa aagccatgat ccgcgcgatg cctgggcctc catctgattc
     2161 atatttcacc gatgaaggta aaccgaatgt ctgacacata tcctcctgtg gcaagcgaag
     2221 taaaaccgtt cgctcagcat tccaacgcct gtcaaaacgt tcgaccgcta acgcgcgcac
     2281 atttcccgct ttaatgattt ctgcgtccgg aacattcaac ccaagttctt tcgccagcag
     2341 cagacagtaa tactcattat caacgctttg gctgagatcg agcgtcgcat tgggctgcct
     2401 gatttcgcca atcggtaatt taatgatgtg cgtcgtcggc gttattcctt tcggaatgca
     2461 ccagtcattg cctattctaa gcagcgctgt cttctcctgc gcgccagcaa ccgagatgcg
     2521 aaagtcattt tcttctctaa tcatgcccag agggatatct gctttataag ccgttaatac
     2581 ttcttcaagc ctggcttcag taagcttttc ccatgccatt atcgggcgcg ttatggtttc
     2641 attttcgggt aataacgtta cggcaccaac gctgtctcgc cctatttctg acaataaatc
     2701 aaacggttgt ctggatttag catgataacg tttaacgatc cggtcacgta caatcgggct
     2761 atcgggtaac aggttatcga agaagttaaa tacggcatct gaggtgatat tccccctctg
     2821 caatggcagc gaaagtgaca acggtctggc ataacggctg gctaaccact ccggtgcata
     2881 cttaaaggtg tgcgcgccgt tggctaactt cgttaactcg cctacccgct ggttgttcat
     2941 ccaagtgaca agtttaggca ttaccactcc agatcttgct gttctgttga ttctggcgag
     3001 gcatttttcg tgtcgcatag tgtcattgag agttcaagcg actgtaaaat cttaaaaaat
     3061 gtcgtgagcg tggtattgtc aggggtgttt tcgaaattgg aaattgtcgc ctgcttaatg
     3121 ccaatttttt tcgccagctc gctctgcgtc cagccatttt gctggcgaac cagtttcatt
     3181 gcattcgcta attgcgttgg gctatagatc ttctgaaagc tcatcatgtc atacgtccac
     3241 agcaaattta tccgcttaag gggatattat cagttttatc ctttagtgag gataaagtca
     3301 atctggaaac acatcttatc cgctgtacgg gataaacgct gcgatatccg cgatcgcgga
     3361 taaaagggag gctttaaagc ctttgaagtc ggcaatatta agccgcatgc catctcgaca
     3421 tgcggcttat acggtttacc agctatattt cactcccagc atgccctgag tatcgctata
     3481 gcctttatca cctagttgca caccgacatt cccccacagg ctaaggttgt tatttacttt
     3541 cgcctcaacc ccggtacgta cttcaccgag attacgcgca ccttcacggc ctacggtttg
     3601 accattcatc ttcacggcgt agactttgct gttgttgatc caattcgctt caatgtaagg
     3661 ctggaactca cgctgtttac catcgtcacg ctggtgatgg ctgttcaggt aggttttcac
     3721 cccaagtcgc gtttgcacat ttccgtcgcc ttccgtttca atgcgcgttc cgtctttccg
     3781 ggtatggtcg gaatctttca cacccatcca ggtgatttgc gcctgtggct ggacgtacca
     3841 ggtattcagc gtcccttcgc tgccgctaaa tgttcccgct tcaaaggtat acccaccctc
     3901 aacagaggcc gtcacaccgc gagaatcata gtcgtcagca gaacggttat cggaactgac
     3961 gctgttatca aaccagttat acagcgccca gctgtcaaca taagcgccgg tcttattcgc
     4021 atcgttctga taccaggtcg cgtacagccc agcgctgtaa ccgctgatgc gcccatccga
     4081 tttataaccc acacgattac tctgagtatt actgtgctga ttagcgtaac ctgccatcac
     4141 gccaagatgc cagcgatcct gcgcgttgct actccactgc gccaaatcgc cgcctagctg
     4201 caatacatag cggttagcct gagtatttag ctgaccgtca ccggcccttg aacgttcgtg
     4261 ccctccgaca tgacgcatcc acatactgct tgccgacccc tgagaatgca gtgaatctgt
     4321 atactgcggc tcacccagac ggtcgtgtaa acgatggcta aacagcgagt tggctgcggc
     4381 aatgttgctg atatagcttc cggcctccgg gcgataaact gatgggcctt ccggatccac
     4441 aacaggggga ttattgatgg gatcgggtgt atccgctggc gttacgccgt cccatttact
     4501 ggtcagatac cagtttttct cgtcattccc cttcccttta gccagcgtgt agacgtaagc
     4561 cccagcttcg acagttccgg tggtcagcgc gaagttacct gcagaattac cgccaacctc
     4621 aatgagttca ataccgttga ccgtttgtgc accgacgccg ccaatgttat caacccgaac
     4681 gcgagtgttc ccttgggtat cgcctttcac gttcatctta tcggtgggcg aattatcgcc
     4741 gcccagcgtc gcgttgaacg tcatcaggcc attgtttccg gtatagttgc cgttaacggt
     4801 cagtacattc cccggtttcc ccccttcgct attcatttga accgtgccgc cattctccag
     4861 gttgccgccg atagtggttt tagcgacacg cagtgccccc ctaacgttca tatcacctgc
     4921 agtggtgaca tttcccgaca tcacaccgct gttatggata ttaatgtgtt gacttgccat
     4981 attaatggca gagtcggaac cgaaagcaat ttcccccccc acgatatccg tcgtaccctg
     5041 atacgccgcg gaatcgttgt taagagtcag gatcccggta cccagttttg tcacatcacc
     5101 gctaccgctg atactgtgcg tcatcgccca gctacgctca gtggagatgg tgagcaggcc
     5161 attattgata acggaagcat cacccaggtt tttctgctcg ttgacgctga gcgcgctgcc
     5221 tgtatcaatg ttgaattgcc ccacaaagcg gctattatcg ccatccagct ctacatctgt
     5281 cctggcggtt gcgctcacga tacccttccc gcttatgcta ttacgcagtt cgccagtcac
     5341 gttttttagc gttaacagac cgtcattggc aatatttcct ctgcctaacc cctgagtatt
     5401 atcgaggctg acttccgcat ttgggctaat gctggttaac gcattgtagc gtgcattaag
     5461 cccctcgata gccagcgttc cccctgtaac attcaggttt ccgccacctg tcagttcacc
     5521 ctgaatgatc ccacctttat ttaccgtcag cgccccctct ttgaacaaaa cagtcgaacc
     5581 catctgcccg gtgaatgtct ctaccgtctg cgtcgatcca ttaagatcga cgatggccgc
     5641 gttgctgatg ttcagttccc gggtgttgcc aagtgcacca tccgcatcgg tacgtaatgt
     5701 ccccatctga acgtaggttg ccccctgata gtcgttctga ccgttggaaa gcgaaacctg
     5761 tcgcaccgta ttgattgcca gatccccttc accaccgatt tttgccgaca tatcggccgt
     5821 tgcgccatag gctccgccat gttcggctaa cgtcagcttt tgcccaccat ggatgttcag
     5881 cgctttcagc ccatagttaa cgtacaaacc atcacctggt gcggtcgtga ggcggaatcc
     5941 ataatcaccc tcggcgacca ccgttccgtt ttgcgcaatg tgtaacgttt tgtccgcttc
     6001 cacctcgtcg ccctgtaaat cacgtaacgt taatgagcca cccgacccaa taaccgtttg
     6061 cgccttcacc agttgaacgc cgacatggct atcgtcgtgt tccagcaaat tgagcgtcgt
     6121 cagagggtta ttcgccatgg gatcattcca cggtttaggc acgtcgataa gcacgtcgcc
     6181 cgtcccgttt acctgatagt tacggccttt ccaggtgtag tcacccgcgc cgacaaccag
     6241 cgtatcgacg ctgatatatc cctctgcaag cgtcgcagca ggaatatccg tatcgaaaat
     6301 gagcgtaccg ccattcatgg ccagtccacc aatggattgc tctcccactt ttaccgatgt
     6361 ggtattttca ctgtcagact gcaacatcgc gtgagtaagc gcagcggtgt tgtcgcgttc
     6421 cagagtgaag gtactgtctt tcagttgggc aacaccggcg aattcagtcc ctgttgcatg
     6481 ggtaaagcca aacatcttgt cgtaggatga cagctgcact cgcatcaagc catcgccttt
     6541 gagcgcattg gtcagcgtgt aatctcctgc actgttcgtt gatgcgagaa tgtcgagcgt
     6601 accggggctt gcaatggtga cttcactcga acccatgccc gttaccccat ctgccgtatg
     6661 agacgcaatg gtcaggaggc cgtcattgac cagcgtttta ccgctataag tattccctgc
     6721 attgaacaca gtatggcctg catcatggat cacttttccg tctttatcgt cacctgtaat
     6781 cagcatatcg acctggtagc cggcatcact gttattagta tgattaaaga caaaaacacc
     6841 ttcgccagaa ccaaactcta ctttcgtcgc gttggttata tacccggcat ctgccgccgc
     6901 ctcaccgtga gctgcaccaa tgtttagcgt tcctacagca ggttcaaaaa cacctaagta
     6961 aacttctcca ccttcgacat tcagcgtgcc gctattcgtc agggttaacg tacccgtacc
     7021 agatgtccct acgtacatat tgaatgtttc aagaagagag ttctgcccat ccaccctcac
     7081 gtcgccctta cttttgtcat tgagagctat ctgtgtatca cgcgctttaa ctatacctcc
     7141 ggtggtaata ttcagttcac ccttgcctaa tacaccgacc tgtagcaatt gtgcgttagt
     7201 agatgatgtc tttaaattcc agagactgtc cgttgaaata ttaactatcc ccttaccgga
     7261 ggcatttcct ccgataaatc catagcccgt tgaattaagg gtggcaccat tactgacatt
     7321 tacgataccg ctaccaaaat taccgacatt gatacccaca gtatcgaacg atgatgtcgc
     7381 atccatcagg ttcagtgtgc cgataccgct tgctttatcg cccaaaaaag taatattttg
     7441 cgatacgaac ttaccgccat cgctaatatt gaggtttccg acgcccgcaa caccaatggt
     7501 ggtatagaca ttcttattga cctcccacat cccccccgtg gtgatgctga cattcccgac
     7561 gccggtttct ccataaccga gtgagcttcc tccgttgctg acaacaagcc cttcattact
     7621 gatattcatt tcgccgtggc cgtcataacc aagattagtt ccgagattag tgataacgga
     7681 gttcttatcc ttaacagtaa tgttgcctgt gcctgtttct ttcatccccg cagtgataat
     7741 tcccgaatct actttacctt cacgcgagac attaagttca ccgtcgccag catcaccgat
     7801 atagatatag cggaaagctt cgcccgttcc gaggaaattc caatgccctt tatcggtcac
     7861 gttgacgaca ccgtgggaac cgtcctgaac gcccactaat gaatattctt tgttattaat
     7921 tagtccatta ttggaaatat tgagtgtgcc attaccgaaa tatccattat agagtctgcg
     7981 taccgtgatg acagagtctt gatcctgcac attcagggtt ccgataccgg tagcattgct
     8041 accgataatc gtgttttcag ccgtaatcag tccaccctcg cgaatagtcg cttccccagt
     8101 tccttgatta ccaatttgaa attcaattga ggaatcgtta ttttttatta gccactcacc
     8161 gcccttttca acgacaacct ggccattact gcccgcctga tagcctaaaa tggcgactat
     8221 tgaactcgtg acgtaaccct tatccgtaat atttaatgag cccgtaccgt aactccctat
     8281 ttcgaataat tcggtcgtca gaacagagtc ctctccctca acattgaccg tcccgacgcc
     8341 tcctgtcgag gaacctagtc ttaaatagcc tccatcaacg tgacctttct gtttaatatt
     8401 cagcgtcccc gttccggatt gacccacatt taaaggcctt gcattatttc cgctatcata
     8461 caatcgccag gtgccaccca aaacattaac ggtgccctct gagtcctcat tagcgccaat
     8521 gacgctggta gtaatagtgg ttaaggaggc attaccgcca gccaaaatat taagcactcc
     8581 agtgccatac cagccaacca ggtaggcatc ataagcaaca tcagtatcaa tattggtaat
     8641 ctgatcatta tcaacttcca gacttgcacc agacgctgaa ccgcttagtg ccagcaaaac
     8701 acccagcgtc aatctactga atttcgttgt cagtccagag gatttacgca aattaaccgt
     8761 cgatgtttta cctgccctgc gagttaattc cgagcaggcc tgaaatacct gtagagtgca
     8821 attccatatc acgcgataga ttctattcat ataaacgctc catatacaaa caatacagcc
     8881 acgaaagaga tgagtcgtca gacattaaaa atgaaactta ttaaattgtc agaggtctgt
     8941 attgagtgtt agttggaggt gggaaggtgt caatttggct aatttgctaa tactttcgaa
     9001 ttgatagatt aaaaaatgca tagttcttat tttatttaaa aatgaactat gagttattgt
     9061 tgattaataa gaaaactgtt tctttcaata ggaaaattat catcatcata tttaacaaag
     9121 aatagcacta attgctaaaa atcgaagttt attaaacccc ttttgcatcc attagtgtct
     9181 ttaggaatat tcgctataaa ataagggttg tgcttaatgc tttaagaaaa atagcaattt
     9241 tcccttgaat atagtactgg tgatggaacg atgaatctgc aagctggctt ttaacaagcc
     9301 agctctaaaa gaagggaaat aagaataact atactcaaaa actaacagcc acggtcatca
     9361 tgatgtggct gtcaatgaaa ctataaccca ggcgctttcc ataacgacgt cgtcagtcca
     9421 tgatcaacca gccccagctg atcctgataa actgcctgcc acttatcgcg tgaatcctga
     9481 taaagttcat gcttttccgg gtctggtgtg tgcgtccgtt cccagcgaac caggcgttct
     9541 ccggtttctg ccattgatga aaaaattccg gcaccgacgc cagctgcaat ggcacatcct
     9601 aatgcagtgg cttctttgac caccggaata ttgacgggta atcccgagac atcagcgaga
     9661 atttgactcc ataatttccc ttttgaacct ccgcctgcaa agactaacga tgaaggatga
     9721 atattcgaga aatcagcaat ttgctgcaag ttacacgctg atacaatcgc cgcattttct
     9781 tccagcgcac ggaacaatgt cgctttgtta catttatccg ggtcaatgga caagttaata
     9841 aaggaaggcg cagcgtgata ccaggtttta aagcgcattc tgtcggagaa gatcggcatt
     9901 acgccccacg acccaggcgg cacccgactg gccatctctt ccagcagcgt ataggtgtcg
     9961 atgcctaaac gttcggcaat cagtttttct tcggcacaga aagcatcgcg gaaccagcgc
    10021 atggtgagtc cggtaaaaaa gcttatagat tcagcttgta ccatgccagg aataacatga
    10081 ggattaacgc gcacgttcat ttctgggtct gtcaccggcg cggctaaatt tacaacttgc
    10141 tgccagaatg tgccgccaag aaccgcggtt tgtgccggac gcacaacgcc taacccaagg
    10201 caaccaagct gcacgtcgcc tcctccaacg accaccggag tgcccgcctt cagaccgcag
    10261 agttccgccg cttgtgaact taccacgccc agcaatgtgc cggtttcttt gacaggagaa
    10321 agaatatcgg cacgcaggcc agccatatcc agcaatgcag gtttccagtc acgggtggtt
    10381 agatcaagaa gtcccgtggt gccagcgtta gagggatcca ccgccagttc gccgctgagc
    10441 atataggcca gccagtcgct gatcatggtg atggttgatg cctgacggta aatatcggaa
    10501 cgatggtgcg ccagccaaag taatctgggg atggcactta aagccagtgt ttgtccggtc
    10561 gcgcgataaa cttcgttttc aaaggtattg ttgtgcagtt ctttaagttc gctaacttcg
    10621 cgtgccgctc tggcatccac attggcgcag gcccagatcg gggctccttc attattatat
    10681 aaaacaatgc cttcacgcat cgaacatgcc gaaacggcag cgatatactc cggggctatg
    10741 ccggcgttgt gcagcgcctg gcgcatacac tcacacgcca gttgccagtt tttgttgaga
    10801 tcaaattcca tagaaccagg aacgtccggt actgccaggt gccgccactc cgcctgtccc
    10861 actgctattt gattgccttc caggtcgaat atcacagccc gaatacttcc ggtacctgca
    10921 tccagcgcca tcaggtaata ctttgattct gaaggggtaa agagtcgagc cattagatta
    10981 tcctcggctt atgcgttctg tctgttcctc tatacgttct ccatcattcc cggtaataag
    11041 gtcatgcaaa tttaactacg taaaatcgcc gctgctgtgt cctgatcggt aaccagtgcg
    11101 ttgatataac cgcctttcat tgcagcggca attgcttcgg ctttattttc tccccctgcc
    11161 acgccaaccc ggacgggtat ggtcttcagc gagcttaaag gtaagccaat cagttcgtta
    11221 tgtattttaa tatccgtgac aacgtcacct tttgcatcaa aaaagtagcc taaaatgtcg
    11281 ccaaccgccc cttttcgacc aatcattaac tgttcgccct ggctgatata accggagcga
    11341 atgatcgtcg cgtcgtcctg ttgactgacg gccccaatgc cgacaatcgc cacatcggct
    11401 gcttgcgcgg ctaacagaac atctttgacg caattttcat tttttagcgt acgggcaatg
    11461 tcagcggagg atgcccgcaa cggagccgga ataatattca cactgcacgc cgcgttaagc
    11521 tgcccgatac ccgtcatata agaaccgacg ccaccggaga gcgtgaccag gcgaatttgc
    11581 tgtgacgaaa taaaaccact taagcgttgc agcgtattca tggttgcctc gccaaaacca
    11641 atcgccagca tctgttgtgg ttgaagtaaa ctcatcaaca tatgcgccgc gcctatcccc
    11701 agtcgcccgc cgacatcagc atccgcaagc ccagggatga cccggacatg ttgcagcgaa
    11761 aactgacgac gtaattgagt ttcatattcc agacagcctt caaagcgaga attaatctgt
    11821 acgcgaataa tgccggactg atgccctttc tccagcaagc gcgacacttt caaacgggtc
    11881 aggccgagac gatcgctgat ctcgctctgg gtcagcccgt cgtgatagta aaaccacgcg
    11941 atccgcgcga cctgttcttc ttcacacatt ccctgttctg aaattgccga atcgttgatt
    12001 gtcataattc attcttcact ttgaacatat ttaaatcttt aatgcaattg ttcagttctt
    12061 gcgcatttat atctgtgatg gcagccacag tttgactcta cgagcatgaa caaacgcaac
    12121 cgtgaaaatc aaaatagcat aaattgtgat ctattcgtcg gaaatatgtg caatgtccac
    12181 ctaaggttat gaacaaatta aaagcagaaa tacatttgtt caaaactcac ctgcaaaact
    12241 gaacggggga aatatgcaaa cgagtgatac ccgcgcgtta ccgctacttt gcgcccgctc
    12301 ggtttataaa cagtattcag gggtcaatgt cctgaaaggc atcgatttta cgttgcatca
    12361 gggggaggtc cacgccctgc tcggcggcaa tggtgccggt aaatcgacgt taatgaagat
    12421 tattgccggt attacccctg ctgatagcgg tacgctggag attgggggca acaactacgc
    12481 cagattaacg ccagttcatg ctcatcagct gggcatttat ctcgttcccc aggaaccgct
    12541 gcttttccca agcctgtcga taaaagaaaa catcctgttt gggatggcaa aaaaacagct
    12601 ctccatgcaa aaaatgaaga acttgctggc ggcgctgggc tgccagtttg atctgcatag
    12661 tctggcagga tcgctggatg tcgccgatcg ccaaatggtg gaaatcctcc gcgggctaat
    12721 gcgcgactcg cggattctga tcctcgatga acctaccgcc tcgcttaccc ctgcggaaac
    12781 cgaacgcttg tttactcgct tgcaagagct gcttgctact ggcgtgggta ttgtttttat
    12841 ctcgcataag ctgccggaaa ttcgccagat tgccgatcga attagcgtga tgcgcgacgg
    12901 aaccatcgcc ttaagcggca aaaccagcga actgtctacc gacgacatta ttcaggccat
    12961 cacgccagtg gtacgggaaa aatcgctctc tgccagccaa aaattatggc tggagttacc
    13021 tggtaaccgc ccacaacatg ctgccggaac gccggtgctg acactggaaa atctgaccgg
    13081 cgaaggtttc aggaatgtca gcatgacgct caatgccgga gaaattctgg gcctggctgg
    13141 gctggtgggg gccggacgca cagaactggc cgagacgctc tatggtctgc gtactttgcg
    13201 tggcggacgc attatgctga atggtaaaga gatcaataaa ttatccactg gagaacgttt
    13261 actgcgcggt ctggtttatc tgccggaaga tcgccagtca tccggactga atctcgatgc
    13321 ttcgcttgcc tggaacgtct gcgcccttac tcataacctt cgtggattct gggcgaaaac
    13381 cgcgaaagat aatgccaccc tggaacgtta tcgtcgggcg ctgaatatta aatttaacca
    13441 accggaacaa gctgcacgga cattatccgg tggcaaccag caaaaaatcc tcattgccaa
    13501 atgcctggaa gcttcgccgc aagcattgat tgtcgatgag ccgacgcgcg gcgtggatgt
    13561 ctcggcccgt aatgatatct accagctgtt gcgcagcatc gccgcacaaa atgtggcagt
    13621 gctgcttatc tcctccgatc tggaagagat cgaactgatg gcagatcgcg tgtatgtgat
    13681 gcatcagggc gaaattgccc actctgcact gaccgggcgc gatattaatg tcgagactat
    13741 tatgcgcgtt gccttcggcg atagtcagcg tcaggaggtg tcatgctgaa gtttattcag
    13801 aacaaccgtg aaatcacggc actgctggcg gtggtgctgc tgtttgcatt acccggtttt
    13861 ctcgaccgcc agtatttaag tgtgcaaacg ctgaccatgg tttatagcag cgcgcaaatc
    13921 ctgatcctgc tggcaatggg cgcgacgctg gtaatgctta cgcgcaatat tgatgtttca
    13981 gtgggttcga ttaccggaat gtgcgcagtg ctgttgggga tgttactgaa cgcggggtat
    14041 tcactacctg ttgcttgtgt cgcgacttta ctgcttggtt tgctcgcggg atttttcaac
    14101 ggtgtcctgg tcgcgtggct aaagatccct gccattgttg ccacccttgg cacgttaggg
    14161 ttgtacagag gcatcatgtt gctgtggact ggcggtaaat ggattgaagg gttacccgcc
    14221 gaactgaaac agctctccgc accgctgctg tttggcgttt ccgcgattgg ttggttgacg
    14281 ataattctgg tggcatttat ggcctggctg ctggcaaaga cggcgtttgg acgcagtttt
    14341 tatgccacgg gcgataattt acagggcgct cgtcaactgg gtgttcgtac ggaagccatt
    14401 cgcattgtgg cattttcgtt gaacggctgc atggcggcac tggcgggaat tgtgtttgct
    14461 tcgcagattg gttttatccc caaccagacc ggtaccgggc tggagatgaa agcaattgca
    14521 gcctgcgtgc tgggcggcat tagtttgctc ggcggttccg gtgcgatcat cggtgcggta
    14581 ctcggcgcat ggttcctgac gcagatcgat agcgtactgg tgctgttgcg cattccggca
    14641 tggtggaacg attttatcgc gggcctcgtg ctgctggcgg tgctggtgtt tgatggacgc
    14701 ctgcgttgtg cgctggaacg taatctacgg cggcaaaaat atgcccgctt tatgacgcca
    14761 ccgccatccg ttaaacccgc ttcgtcaggt aaaaaacggg aggccgcata atgcgtattc
    14821 gctacggttg ggaactggct cttgccgcac tgctcgttat tgagattgtc gcatttggtg
    14881 caattaaccc gcgaatgtta gatctcaata tgttgctgtt cagcaccagt gactttatct
    14941 gcattggcat tgtcgcccta ccgctgacga tggtgattgt cagtggcggg atcgatattt
    15001 cgtttggttc gaccatcggc ctctgcgcca ttgcattggg cgtactgttt caaagtggtg
    15061 tgccgatgcc gctggcgata ctcctgacct tactgctcgg cgcattgtgc gggctgatca
    15121 acgccggatt aattatctat accaaagtta acccgctggt gattacgctt ggcacgctgt
    15181 atctgtttgc cggaagcgct ctgctgcttt ccggtatggc cggagcgacg gggtacgaag
    15241 gtattggtgg attcccgatg gcgtttacag atttcgctaa cctggatgtg ctgggactcc
    15301 ccgttccgct gattatcttc ctgatatgtc tcctcgtttt ctggctctgg ctgcataaaa
    15361 cccatgccgg acgtaatgtg tttttgattg ggcaaagccc gcgcgtggcg ctttatagcg
    15421 cgattccagt taaccgcacc ttatgtgcgc tctatgccat gacggggctg gcgtctgcgg
    15481 tcgccgctgt gctgctggta tcgtattttg gttcagcacg ttccgatctc ggtgcgtcgt
    15541 ttctgatgcc cgccatcacc gccgtggtgc ttggcggtgc caatatttat ggtggttccg
    15601 gttccattat cggcaccgcc attgcggttt tattagtggg atatttgcaa caaggtttgc
    15661 aaatggcagg agtgccaaat caggtgtcca gcgccctttc cggtgcgcta cttatcgtcg
    15721 ttgtcgtagg tcgttccgtt agcctgcatc gccagcaaat taaagagtgg ctggcgcgtc
    15781 gggccaataa cccattgcca taaaggatat cttcatgaca cttcatcgct ttaagaaaat
    15841 cgccttactt agcgctcttg gcattgccgc aatctctatg aatgtgcagg ccgcagagcg
    15901 tattgcattt attcccaaac tggttggcgt gggatttttt accagcggtg gcaacggcgc
    15961 acaacaagcg ggtaaagagc tgagcgttga tgtgacctac gacgggccga cagaacccag
    16021 tgtttctggt caggtacagt tgattaataa cttcgtcaat caaggttata acgccattat
    16081 cgtttctgcg gtttcgcctg atggcttgtg tccggcactg aaacgcgcca tgcaacgtgg
    16141 tgtgagagtg ctgacctggg actctgatac taaaccggag tgccgctctt actacattaa
    16201 tcagggaacg cccgcccagt tgggaggtat attggtggat atggcggcgc gtcaggtgaa
    16261 taaagacaaa gccaaagtcg cgtttttcta ctcaagcccc accgttacgg accaaaacca
    16321 gtgggtgaaa gaagcgaaag cgaaaatcgc caaagagcat cctggctggg aaattgtcac
    16381 tacgcagttt ggctataacg atgccactaa atcattacaa accgcagaag gaatattaaa
    16441 agcgtatagc gatctcgacg ccattatcgc ccccgacgcc aacgccctgc ccgctgccgc
    16501 acaagccgca gaaaacctga aaaatgacaa agttgcgatt gtcggattca gtacgccaaa
    16561 cgtgatgcgt ccatatgtgg aacgcggcac ggtgaaagaa tttggcctgt gggatgtggt
    16621 tcagcaaggc aaaatttcag tgtatgtcgc ggatgcatta ttgaaaaaag gatcaatgaa
    16681 aacgggcgac aagctggata tccagggcgt aggtcaggtg gaagtctcgc caaacagcgt
    16741 tcagggctat gactacgaag cggatggtaa tggcatcgta ctgttaccgg agcgcgtgat
    16801 attcaacaaa gagaatatcg gcaaatacga tttctgatgt gcattactta accggagtaa
    16861 gttatggcag atttagacga tattaaagat ggtaaagatt ttcgtaccga tcaaccgcaa
    16921 caaaatatcc cttttaccct gaaaggttgc ggtgcgctgg attggggaat gcagtcacgc
    16981 ttatcgcgga tatttaatcc gaaaacgggt aataccgtga tgctggcctt tgatcatggt
    17041 tattttcagg ggccgactac cggacttgaa cgcattgata taaatatcgc cccgctgttt
    17101 gaacatgccg atgtattaat gtgtacgcgc ggcattttgc gcagcgtagt tcccccagcg
    17161 accaataagc cggtggtact gcgggcgtca ggtgcgaact ctattctggc ggaattaagt
    17221 aatgaagccg tggcgttatc gatggatgat gccgtgcgcc tgaacagttg cgcggtggcg
    17281 gcgcaggttt atatcggcag cgaatatgaa catcagtcga tcaaaaatat tattcagctg
    17341 gttgatgccg gaatgaaagt gggaatgccg accatggccg tgactggcgt gggcaaagat
    17401 atggtgcgcg atcagcgtta tttctcgctc gcgactcgaa tcgccgctga aatgggggcg
    17461 caaattatca aaacctatta tgtcgaaaaa ggttttgaac ggattgttgc cggatgtccg
    17521 gtacccattg ttattgctgg cggtaaaaaa ttaccggagc gcgaggcgct ggaaatgtgc
    17581 tggcaggcta tcgatcaggg cgcttctggt gtggatatgg ggcgtaatat tttccagtct
    17641 gaccatccgg tggcgatgat gaaagccgta caggcggtgg ttcaccataa cgaaacggct
    17701 gatcgggcat atgaactcta tctgagtgaa aaacagtaac tgcggattta aggagaagaa
    17761 ttatgcacgt cacactggtt gaaattaacg ttcatgaaga caagattgac gagtttatcg
    17821 aagtttttcg ccagaaccac ctgggctctg tacaggaaga aggcaatttg cgcttcgatg
    17881 tcttacagga cccagaagtg aattcgcgct tttatattta cgaagcctat aaagatgaag
    17941 acgcagtggc attccataaa accacgcctc actacaaaac ctgtgtcgcg aaactggaat
    18001 ctttaatgac cgggccgcgt aaaaaacgtc tgttcaatgg gttgatgccg tgaggcgaat
    18061 ttatcatttt tatctacaat tggggtaacg cgctgacggg agtaaaaata tgtctgactg
    18121 gaacccctct ttatatctgc actttgccgc tgaacgatcg cgtccggcgg tggagctgct
    18181 tgccagagtg ccgctggaga atatcaaata cgttgccgat cttggctgtg gcccaggtaa
    18241 cagcaccgcc ctgctgcatc aacgttggcc tgcggccagg ataacaggca tcgactcgtc
    18301 tccggcgatg attgctgaag cgcgcagtgc tttgccagac tgccagttcg tggaagcaga
    18361 catccgcaac tggcaaccgg aacaggcact cgatctgatt tttgctaatg cctcactgca
    18421 atggctgccc gaccactacg aattgtttcc tcatctggtt tctttactta atccgcaggg
    18481 cgtgctggca gtacagatgc cagataactg gctggagccg acccatgtgc tcatgcgcga
    18541 agttgcctgg gaacaaaact acccggatcg cgggcgtgag ccgttggctg gcgttcatgc
    18601 ttactacgat attttgagcg aagccggatg tgaggtcgat atctggcgaa ccacctacta
    18661 tcaccagatg ccgtcacacc aggcgattat cgattgggtg actgccactg gattacgtcc
    18721 gtggttacag gatctgatcg agagcgaaca gcagcttttt cttaagcgct accatcagat
    18781 gctggaagag cagtatccac tgcaagagaa cggacagata ctgctggcat ttccgcgtct
    18841 ttttattgtt gcccggcgta tggagtaaat atcacgtcag ctggtaatga cgatcgggaa
    18901 gaatttttgc tggaatttcg gcttcatcgt tcatctgtaa caggtcaatt tcaatagcgt
    18961 tgcagatggc atccagcggt aaatcattgt tttcagtacc gaacggatct tccagttctt
    19021 ccgccagaca atccagcgaa ataaaagtgt aggaaatcag cacagagata aaaggcgtca
    19081 tgtaatgcag gtccacgacc agcgcgaacg gcagcatgat acaaaacaga taaacggtac
    19141 gatgcaaaat cagggtgtag gcaaagggaa ttggcgtata ggcaatgcgc tcgcatcctg
    19201 ccaggaccgc tgaaatatca ttaagccgat cgttgaggct aataaacagg atatctgaaa
    19261 gctgcccatt gcggcgctga accgccaacc actcccccat tattaacaag atacggttag
    19321 ctggagagtt cgaagccagt acaggctgaa gatcttcagt cttgagataa tgagccagca
    19381 cttccgcctg tggctgtttg cgtaatgtca tgcgtaaaca gtgggcgaag gcgatttgca
    19441 accgggcaaa ctcccttaca cttgccgaat ccggcaatgt cgtttttacc tcgcgcagta
    19501 acgaccgtga ggcaatcatc aactgccccc aaagttttcg cgcttcaacg taacgggcgt
    19561 acccggcatt attacgaaaa ccaagaaaaa tggcgatggc gacaccgaga atgctgaacg
    19621 gtgcgagggt gaatttgatg cccagatgcg tgtaccaggg cagcatgaaa ataacagcga
    19681 tagaaaagag aaaattgagt agtaagcgcg atgatatctt ggataatact gagccgtgcc
    19741 agacaaaaat acggcgcagc cagtgttgtt gtggacgaac aatcatggtt atcttcaggc
    19801 gtgaaaaagt cgccctatta aacgtgatta cgatcacatt ctcaagacgc tacttacaaa
    19861 ttacctacaa gcttacaaca ttaacaggcg ctgcatgtgg catctgttaa tgataaaggc
    19921 acataggatg ttgtaactaa tatagtgaaa taagaaaccc ggtcgaaacc gggttcagaa
    19981 gtaacggtgt tattagcaca acggacgtac agcttcgcgc atcccttttt caagaatcgc
    20041 atccaggtca ttagcaacct gctcgaccag accaggcact tgcgtcaggt cctgctccca
    20101 gtggtctttt tctgccaaca caatcgctac cagttcctgg gtaccgataa cacggtcacg
    20161 atgctggctc cagagttgct gataacgttc cagccagtgt gcatcgtcct gtaccggata
    20221 tgtttctccg ttacgctcac cacgatagaa ggcaatcaat gccgctaatg cgaaagtaag
    20281 gcgcgccgga agtgtgcctt ttgccttctg ccctgccagc agctgcggca ggatgcgagt
    20341 gcggaacttg gtcataccgt tgagcgcgat agacagcagc tgatgcttaa tgtacgggtt
    20401 acggaaacgc ccggtgactg cactggcgaa agattccagt tcatcacgag gcaaatccag
    20461 taccggaata atttcttcgt agatagcttt ttcaacgaat gcgcaaattt cagcatcgtt
    20521 catcgcttca cctacggtat ccagccctgc ctggaacgcc accggcacca gcgcggtgtg
    20581 cgcaccgttg aggattgcca ctttgcgttc tttgtacggt ttaatatcgt caacaatcag
    20641 cacgttgagc ggatatttgt ccagacgcag ttcagtcgct aaggatttcg gcccctgaat
    20701 cacaaacagg taaaagtgtt cagcggtgtc gaggaaacca tcgtgataac ccagttcttc
    20761 ttccagttta gccacttcat cgcgcggata accggtaacg atacggtcca ccagcgtaga
    20821 acagaagctg ttagcctgat ccaaccattg aataaatgct tctggcagtg cccactcttg
    20881 agcatagcgc agcaccagtt cacgcaacgc gtcgccattg tagtcgatca actcacacgg
    20941 gatgatgatc caacctttat ccagcgcacc gttgaaatgg ctaaaacgtt cgaacagcag
    21001 acgggtcagt tttgcgggat agcttaccgc tggcgcgtca tcgaatttat cgcccgcatg
    21061 gtagctgata cccgcttcgg tggtgttaga gaaaacaaag cgcatttccg gattgtgcgc
    21121 cagtttcagg aattcatcgt attcactgta gacgctgatt tcacgattaa cagagcgaat
    21181 cagacgcgcg tcgctgaccg cttccccctt ctcattcagg ccacggataa tggtggtgta
    21241 cagaccatcc tgcgtgctca gtgacggcgg gaatgaagtt tcaatcggac gaacaacgac
    21301 cacgccagaa ttcagatcgg tgtgctcatt caggagatcg atttgccagt caacaaaggc
    21361 gcgcaggaag ttaccttcac caaactgaat gatacgttct ggatactgtg caccgggaaa
    21421 atcgcgacga tttagtgttt tcacaatggg ttcccttctg attagtcata caacctgttt
    21481 gaattggtac gacaggttag caaactttaa tacgccgaac ccctgttttg atcaactcct
    21541 gatgattaat gagcagtttt atgagaaaag tgtggcgcgg atcatggttt aatcgaggaa
    21601 atacgccttt tcctggatca taaagtggta gaacacattg cattcaaatc gcgcggaatg
    21661 aataaagatg tcagacaact tcctcaccgt aacgcatagt gctggtacgg ttgcgcccat
    21721 ctttcttcga gcgatacaga cacgtatcgg cttcgaccat caatttatta aaatcatcgg
    21781 tcagcgttcg gtacgatgcg cgaccactac cgacgccaat acttaccgtg agataaagcg
    21841 ttttttgttg ccaggtgaat ggttgcagtt caacgccttt acggattttt tccgccatta
    21901 gcagaccatc gacaggattc accgacggca ctgcaacagc aaattcttcg ccgcccatgc
    21961 gcgccaccag ccccttatca ccgacaatct tctgaatatg ccgggcaaac acgcttaaca
    22021 ctttatcgcc acattcatgt ccatagttat cgttaatgct tttgaaatag tcaatatcaa
    22081 gcagcatgac cgtcagatgt tgtgtctgtt tcagcgatgg acttttcagc gcctcataaa
    22141 gaccggaccg tgagtaaacc tgagtcagaa aatcaaagtc ggctcgcagc gcaacttgct
    22201 tcattagcga attgatcgct gccacgctaa aagaaaccat aattgggcat atcgccatcg
    22261 tggcaatacc gagacgtgcg gagaacattt ccggaatgga gaacggcgaa ccgaccgaga
    22321 tatcaatcac cgaatttgcc accagtacga tttccaccgc accggtgaca aaggtcaaca
    22381 gacatgttac ctgcggcgta tagcgcactg cacaccagat taatgcaggc agcgggaacg
    22441 ccagactccc cgctccgcca atgactaccg aggcaataac cgacacaata agcgcgatgg
    22501 cgggcatcat ctgttctgct ttaaagcgcg gcagcactcc aggaatagcc aacgtcagca
    22561 tacaaggcac gatcaacacg cccgttgaga attgctcact gaaccagtcc gcaagcaaag
    22621 gccagaaatc cagactatca atactgaccg aaccaatcgc cccgacaata gcgcataata
    22681 atgcggcaat cagacagtaa ttaaatagcc gtaaggcact tactggctca tacttatttt
    22741 tccccagacg cttatcccgc acgaccagta aggcgacggt aacaataaac atcatattgg
    22801 agaaattgat aaccagtgaa accagtcccc attcggtggt tatggcatca taaaccagca
    22861 tcgccacata actgatcgca tagtaatgca gtcgattaag ccagacatag cgggcaaaca
    22921 cccccgccat tacgccattc agcggccaga aaagagaaag ttcgtgaacc agccgtaact
    22981 ctgcaccaat aaaatagaac agcgtggtta gcgcaaaaat agcgatgcta ttacgtagca
    23041 gatgaccttc ctggaacaaa cggaaagttg aaatggggtg tacatgcatt aattatctgt
    23101 ccatacaatt gcgggtggca attatctcaa cgtaataagt aaattcacat aaaggatagc
    23161 atgttcgcta acagcggcgg caaataaagg taccctggga atgctatttt atcgttttcc
    23221 gcaattctca tggctgattc atctgtacaa ccagccagtg atagattacc gccacgatat
    23281 aacgttgttg ttcttcgctt aacgaaacac cctgcggaat gttatgccac ttagccagac
    23341 aaccgcgaca gcaggtcgcg gtggcgtgct gagcgataaa caccggatgc ccgcgcatgg
    23401 gggtttgctt accgtcatta gccggtaatg ccggagcaag acgtttagcg acaaaatccg
    23461 ccgcatgttg ttcgatgact ggcgcgcctt tctccaggca atactgacgc tctttcatgc
    23521 ccagacgaaa gcgagagcga aattttgaac gggataaacg cgcgaagagt ggatcgagag
    23581 actgcattaa taaaccgaac gccctaactg tttcgtcaat tgttcaagga cggcgatacc
    23641 cgcaagcgag ttacctgcat catccagttc cggactccag actgcgatgg ccatttcatg
    23701 cggaacaatc gccacaatac cgccaccaac gccagatttc gccggtagcc ccacccgcca
    23761 ggcaaactcc cccgcgttct ggtacatacc actggtcgcc atcagcgcgt taatttgccg
    23821 cgcctgcatt ggcgtcacca caggttcatc aatatgaata gctttcccct gattagccag
    23881 aaagacaaac gtccgggcca gctctacaca gctcattttc agagcgcagt aatgaaagta
    23941 gttttgcaga acggttgtca catcatgatg gaaattgcca aacgacttca tcagccaggc
    24001 gatagccgca tttcgcgcgg aatgttcaaa ttcggaacgc gctaccaccg tatcgtatga
    24061 aatatcagac acaccgctta agccgcgcac gacttccagc atacgttgcc gtggtgcgct
    24121 taatcgccct tgcaacatat cgcaaaccac cagcgcacca gcattaataa acggattacg
    24181 cggaataccc tgctccattt ccagttgcac taaagaattg aacggtgatc cagacgggtc
    24241 tttgccgacg cgttgccaga tttcctcttc ggagtaatga cgcatggcga caacgagact
    24301 cagcactttg gaaatagact gaatggaaaa acgttcttgc gcgtctccgg cctgaaaaag
    24361 ctgtccgtca acggtacaga tagcaatgcc caatcgggaa ccgtctactg tagccagcgc
    24421 cggaatataa tccgcgactt taccctgacc aatgagcggc cgcacttgcc gcaagatgtt
    24481 tcctaaaatt gcattatcca tggcgactgc cactttctac tcctggaccg caggtctgaa
    24541 aagacctgcg agtatatcag agctgaatat gtcgcgtcag atccggtctt tccacaccgt
    24601 ctggatatta cagaattcgt gtaagccgaa atgggaaagc tcacgaccaa agccactctt
    24661 tttcacgcca ccaaaggcca ctcgcgcgtc gctggcacaa taaccattga taaacacccc
    24721 accgcattcc agacgtgccg ccatctgtct ggcctgtgtt tcgtcggtgg taaaaatggt
    24781 cgctgaaagg ccgaactcac tatcattagc cagttccagc gcatgttctg catctttcgc
    24841 aacggtgatt gccgcaacag ggccaaacat ttcttcccga aacgcggtca tttctggggt
    24901 aacattcgcc agaaccgttg gcggatagta gttacctgcc ccagccatct tttccccgcc
    24961 cagtaacaaa cgcgcaccct gcgccagggt tttctccacc tgatgatgca gctcatcacg
    25021 taaatcaaaa cgagccattg gtccgagagc gttctcttca tcacggggat cgcccatttt
    25081 caaggctgcc gcagctgcca caaaacgttc ggtaaatgca gaagcaattc cctcttcgat
    25141 aataaagcgt tttgccgctg cacaaacctg tccggtattc tgataacgtc cggctaccgc
    25201 cgctttaacc gccagttcca gatcggcatc gttaagcaca ataaatggat ccgaaccgcc
    25261 cagttccagt acgcattttt tcagtgccgc tccagcctgt gcgccaatag ccgctcccgc
    25321 acgaacactt ccggtcaccg tgacagcagc aatgcgcgaa tctttaatca tttgactgac
    25381 accgtcgttg tcggcattca gccagccata tacgccttgc gggattcccg catctttaaa
    25441 cacctgggca atgagctgag cacagcccat cacattcggc gcatgtttaa gtaagtagcc
    25501 gttacctgca agaatgatgg gaaccgcgcc acgcatcacc tgccataacg gaaagttcca
    25561 cggcataatc gccagaatcg tccccaacgg tcgatactca ataactgcct gctgattttc
    25621 caccagcgta ggttccgcct tcagcattgc cggaccatgt tctgcatacc agtcacacaa
    25681 attcgccgat ttcgccactt cagcgcgcgc ctgattgatt ggtttgccca tttcacgggt
    25741 gatcatttgc gccatttctt cgctacgggc gcgcagagcc ttaccgatac cacgcagttt
    25801 ttcagcacga taatctatat ttgtctcgcg ccagtcgcga aagcctgctg ccgccagctg
    25861 aagtgcgttt tcgatatcgt tagcgccagc ccaaggcagc acagaaagtt gttcacccgt
    25921 ggcaggattt attgaaattg catgagttgc cggagtaatg gtcatcgggg tatctccttt
    25981 atgagtcatg gtatgaagat acgcagattt actcttgctt taaaatgaat aatattaagc
    26041 cacttattca cgaatcgaga atgttatgga tctgacccaa ctggagatgt tcaacgcggt
    26101 tgccgaagct ggcagcatta cccaggctgc agcaaaagtg catcgcgtac cgtcgaatct
    26161 cactacccgt ctacgccagc tggaaacaga actgggggta gatctgttta ttcgcgagaa
    26221 tcagcgttta cgtctctctc ctgccgggca taacttttta cgctatagcc aacaaattct
    26281 cgcgttagtg gatgaagcgc ggagcgttgt cgctggcgat gagccgcaag gtttattttc
    26341 tcttggttcg ctggaaagca ccgcagcagt gcgcattccg gccacgctgg cggaattcaa
    26401 ccatcgttat cccaaaattc agttttcact ttccaccggc ccttccggca ctatgctgga
    26461 aggagtgctg gagggaaaac tgaatgcggc atttattgat gggcccatta accatactgc
    26521 cattgacggg ataccggtat accgcgagga gctgatgatc gtcacgccac aaggtcatgc
    26581 gccagtaatc cgcgccagtc aggttaacgg cagtaacatt tatgccttcc gcgccaattg
    26641 ttcgtatcgt cgccacttcg agagctggtt tcatgctgac ggtgccgctc cgggaactat
    26701 tcacgagatg gagtcgtatc acgggatgtt ggcctgtgtg gtcgcaggag caggcattgc
    26761 gcttattcca cgctctatgc tggaaagtat gccggggcat caccaggttg aagcgtggcc
    26821 gttagctgag caatggcgtt ggttaacaac ctggctggtc tggcgtcgcg gtgcgaaaac
    26881 gcgtccgctt gaggcattta ttcaactact ggatgtgcct gactcggcaa aacagggtta
    26941 ccaatgagct atttttgata gttcttgcag ctcaaatgcc ctatatagtg atgttccgat
    27001 gacttatgac tatatggggc aaatatggtt acgccagtaa gcatcagtaa ttacatatct
    27061 cttcctgatg attttcccgt acgtaatatt gcacctcagg taaaagaagt tttaaaggac
    27121 tttattgatg cgctaagtac aataatatgt gatgaagagt ggcgcacgtc tttaaacatc
    27181 aactcagcca caaaaaagat atttaataac cttgataact tatcatatat tcagaggact
    27241 tcttttcggg gta
//
LOCUS       DAPHDW010000054        26869 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.054, whole genome shotgun sequence.
ACCESSION   DAPHDW010000054 DAPHDW010000000
VERSION     DAPHDW010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26869)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26869)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26869
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.054"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            4..768
                     /gene="ycaL"
                     /locus_tag="RH057_002252"
     CDS             4..768
                     /gene="ycaL"
                     /locus_tag="RH057_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HDV3128612.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
     gene            941..1624
                     /gene="cmk"
                     /locus_tag="RH057_002253"
     CDS             941..1624
                     /gene="cmk"
                     /locus_tag="RH057_002253"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HDV3128613.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQVIEKALQ
                     YARQKLALA"
     gene            1735..3408
                     /gene="rpsA"
                     /locus_tag="RH057_002254"
     CDS             1735..3408
                     /gene="rpsA"
                     /locus_tag="RH057_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HDV3128614.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            3568..3852
                     /gene="ihfB"
                     /locus_tag="RH057_002255"
     CDS             3568..3852
                     /gene="ihfB"
                     /locus_tag="RH057_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HDV3128615.1"
                     /translation="MTKSELIERLATQQSHISAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            4059..6323
                     /locus_tag="RH057_002256"
     CDS             4059..6323
                     /locus_tag="RH057_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309023.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
                     /protein_id="HDV3128616.1"
                     /translation="MKITTVGVCIICGIFPLLILPQLPGTLTLAFLTLFACVLAFIPV
                     KTVRYIALTLLFFVWGILSAKQILWAGETLTGATQDAIVEITATDGMTTHYGQITHLQ
                     GRRIFPAPGLVLYGEYLPQAVCAGQQWSMKLKVRAVHGQLNDGGFDSQRYAIAQHQPL
                     TGRFLQASVIEPNCSLRAQYLASLQTTLQPYPWNAVILGLGMGERLSVPKEIKNIMRD
                     TGTAHLMAISGLHIAFAALLAAGLIRSGQIFLPGRWIHWQIPLIGGICCAAFYAWLTG
                     MQPPALRTVVALATWGMLKLSGRQWSGWDVWICCLAAILLMDPVAILSQSLWLSAAAV
                     AALIFWYQWFPCPEWQLPPVLRAVVSLIHLQLGITLLLMPVQIVIFHGISLTSFIANL
                     LAIPLVTFITVPLILAAMVVHLSGPLILEQGLWFLADRSLALLFWGLKSLPEGWINIA
                     ERWQWLSFSPWFLLVVWRLNAWRTLPAMCVAVGLLMCWPLWQKPRPDEWQVYMLDVGQ
                     GLAMVIARNGKAILYDTGLAWPEGDSGQQLIIPWLHWHNLEPEGVILSHEHLDHRGGL
                     DSILHTWPMLWIRSPLNWEHHQPCVRGEAWQWQGLRFSVHWPLQASNDKGNNHSCVVK
                     VDDGTNSILLTGDIEVPAEQKMLSRYWQQVQTTLLQVPHHGSNTSSSLPLIQRVNGKV
                     ALASASRYNAWRLPSNKVKHRYQQQGYQWLDTPHQGQVTVNFSAQGWRISSLREQILP
                     RWYHQWFGVPVDNG"
     gene            6360..8108
                     /gene="msbA"
                     /locus_tag="RH057_002257"
     CDS             6360..8108
                     /gene="msbA"
                     /locus_tag="RH057_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HDV3128617.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DSLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            8105..9091
                     /gene="lpxK"
                     /locus_tag="RH057_002258"
     CDS             8105..9091
                     /gene="lpxK"
                     /locus_tag="RH057_002258"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HDV3128618.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHADVSALV
                     STGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            9128..10360
                     /locus_tag="RH057_002259"
     CDS             9128..10360
                     /locus_tag="RH057_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HDV3128619.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            10412..10594
                     /gene="ycaR"
                     /locus_tag="RH057_002260"
     CDS             10412..10594
                     /gene="ycaR"
                     /locus_tag="RH057_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HDV3128620.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            10591..11337
                     /gene="kdsB"
                     /locus_tag="RH057_002261"
     CDS             10591..11337
                     /gene="kdsB"
                     /locus_tag="RH057_002261"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HDV3128621.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGADR
                     IIVATDHEDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQPSPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            11491..12384
                     /locus_tag="RH057_002262"
     CDS             11491..12384
                     /locus_tag="RH057_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HDV3128622.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(12361..13140)
                     /gene="elyC"
                     /locus_tag="RH057_002263"
     CDS             complement(12361..13140)
                     /gene="elyC"
                     /locus_tag="RH057_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HDV3128623.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            13276..14061
                     /gene="cmoM"
                     /locus_tag="RH057_002264"
     CDS             13276..14061
                     /gene="cmoM"
                     /locus_tag="RH057_002264"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HDV3128624.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     HQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            14058..15380
                     /gene="mukF"
                     /locus_tag="RH057_002265"
     CDS             14058..15380
                     /gene="mukF"
                     /locus_tag="RH057_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HDV3128625.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            15361..16065
                     /gene="mukE"
                     /locus_tag="RH057_002266"
     CDS             15361..16065
                     /gene="mukE"
                     /locus_tag="RH057_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415443.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HDV3128626.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEENQPDSGEEE"
     gene            16065..20525
                     /gene="mukB"
                     /locus_tag="RH057_002267"
     CDS             16065..20525
                     /gene="mukB"
                     /locus_tag="RH057_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HDV3128627.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEADLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADCAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFTS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLCERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLPGSDEAPSQAS"
     gene            20786..22633
                     /gene="ldtD"
                     /locus_tag="RH057_002268"
     CDS             20786..22633
                     /gene="ldtD"
                     /locus_tag="RH057_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV3128628.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPVKGTRWLYSSKPYALATPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLALLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLVYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWQVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADGRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            22814..23362
                     /locus_tag="RH057_002269"
     CDS             22814..23362
                     /locus_tag="RH057_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HDV3128629.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSPGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            23389..24036
                     /gene="gloC"
                     /locus_tag="RH057_002270"
     CDS             23389..24036
                     /gene="gloC"
                     /locus_tag="RH057_002270"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HDV3128630.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(24258..25448)
                     /gene="aspC"
                     /locus_tag="RH057_002271"
     CDS             complement(24258..25448)
                     /gene="aspC"
                     /locus_tag="RH057_002271"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HDV3128631.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGIPEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSETVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSNDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCEAI
                     VAVL"
     gene            complement(25633..26721)
                     /gene="ompF"
                     /locus_tag="RH057_002272"
     CDS             complement(25633..26721)
                     /gene="ompF"
                     /locus_tag="RH057_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HDV3128632.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKAVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
BASE COUNT         6498 a         6588 c         7290 g         6493 t
ORIGIN      
        1 cacatgaaga atactaaatt actgctggcg attgcgacct ctgcagcatt actgacaggg
       61 tgtcaaaata cccacggtat tgataccaat atggctatca gctccggttt aaatgcctat
      121 aaagcagcaa cattaagcga tgccgatgca aaagcgattg ccaatcaggg ctgtgccgaa
      181 atggacagcg gcaatcaagt cgcaagtaaa tccagcaagt acggtaaacg tctggcaaaa
      241 atcgccaaag cattgggtaa caatattaat ggcacgccgg tcaactataa ggtttatatg
      301 accagcgacg tcaacgcatg ggcgatggcg aacggctgtg tccgtgtcta cagtggcctg
      361 atggacatga tgaatgacaa cgaaattgaa ggtgttctgg gccatgaact gggccacgtc
      421 gcgttgggtc actcgctggc tgaaatgaaa gcttcttatg cgatcgttgc cgcacgcgat
      481 gccatttcag ctaccagcgg tgtggcttcc cagctttccc gctcacaatt gggtgatatc
      541 gcagaaggcg ctattaatgc taaatactcc cgtgataaag agtccgaagc agatgatttc
      601 tcctttgatc tgttgaagaa acgtggcatc agcacccagg ggctggttgg cagctttgaa
      661 aaactggcta gtctggatgg cggtcgcacc cagtccatgt ttgactctca cccaccatca
      721 acagagcgtg cgcaacacat ccgtgatcgt atcgcctctg gtaagtaaat cattgtcatc
      781 tttcgggctg gtcttctgcc agcccgctat aattgcgcaa taaatcccca tctgaataca
      841 gacaaaactg gtttttgcac acaacgttaa cgatttgtgg cgtcggcgcg tataatgcgc
      901 gcggttatgt taacggtacg cctgttttaa ggagataaag atgacggcaa ttgccccggt
      961 tattaccatt gatggcccaa gcggtgcagg gaaaggcacc ttgtgtaagg ctatggcgga
     1021 agcattgcaa tggcatctgc tggattcggg tgcaatttat cgcgtactgg cattggcggc
     1081 attacatcac catgttgatg ttgcgtcaga agatgcgctg gtaccgctgg catcccatct
     1141 ggatgtacgt tttgtgtcaa ccaatggcaa tctggaagtg atcctcgaag gggaagatgt
     1201 cagcggcgaa attcgtactc aggaagtggc gaatgcagct tcacaagtcg cggcattccc
     1261 acgcgttcgt gaagcattat tgcgtcgcca acgcgcgttt cgcgaattac caggcctgat
     1321 tgccgatggc cgcgacatgg gaacggtggt attccctgat gcgccagtga aaattttcct
     1381 tgacgcctcc tcggaagaac gtgcgcatcg ccgcatgcta cagttgcagg agaagggctt
     1441 tagtgttaac tttgagcgcc ttttggccga gatcaaagaa cgcgacgacc gcgatcgtaa
     1501 ccgagcggta gcgccactgg ttccggcagc cgatgcttta gtgttggatt ccaccacctt
     1561 aagcattgag caagtgattg aaaaagcgct acaatacgcg cgccagaaat tggctctcgc
     1621 ataagcgacc gaatttgcag tacccccgtt gcaatggaat gacagcgggt atgttaaaca
     1681 accccatccg gcatggagcc aggtggacgt taaatataaa cctgaagatt aaacatgact
     1741 gaatcttttg ctcaactctt tgaagagtcc ttaaaagaaa tcgaaacccg cccgggttct
     1801 atcgttcgtg gcgttgttgt tgctatcgac aaagacgtag tactggttga cgctggtctg
     1861 aaatctgagt ccgccatccc ggctgagcag ttcaaaaacg cccagggcga gctggaaatc
     1921 caggtaggtg acgaagttga cgttgctctg gacgcagtag aagacggctt cggtgaaact
     1981 ctgctgtccc gtgagaaagc taaacgtcac gaagcctgga tcacgctgga aaaagcttac
     2041 gaagatgctg aaactgttac cggtgttatc aacggcaaag ttaagggcgg cttcactgtt
     2101 gagctgaacg gtattcgtgc gttcctgcca ggttctctgg tagacgttcg tccggtgcgt
     2161 gacactctgc acctggaagg caaagagctt gaatttaaag taatcaagct ggatcagaag
     2221 cgcaacaacg ttgttgtttc tcgtcgtgcc gttatcgaat ccgaaaacag cgcagagcgc
     2281 gatcagctgc tggaaaacct gcaggaaggc atggaagtta aaggtatcgt taagaacctc
     2341 actgactacg gtgcattcgt tgatctgggc ggcgttgacg gcctgctgca catcactgac
     2401 atggcctgga aacgcgttaa gcatccgagc gaaatcgtca acgtgggcga cgaaatcact
     2461 gttaaagtgc tgaagttcga ccgcgaacgt acccgtgtat ccctgggcct gaaacagttg
     2521 ggcgaagatc cgtgggtagc tatcgctaaa cgttatccgg aaggtaccaa actgactggt
     2581 cgcgtgacca acctgaccga ctacggctgc ttcgttgaaa tcgaagaagg cgttgaaggc
     2641 ctggtacacg tttccgaaat ggattggacc aacaaaaaca tccacccgtc caaagttgtt
     2701 aacgttggcg atgtagtgga agttatggtt ctggatatcg acgaagaacg tcgtcgtatc
     2761 tccctgggtc tgaaacagtg caaagctaac ccgtggcagc agttcgcgga aacccacaac
     2821 aagggcgacc gtgttgaagg taaaatcaag tctatcactg acttcggtat cttcatcggc
     2881 ctggacggcg gcatcgacgg cctggttcac ctgtctgaca tctcctggaa cgttgcaggc
     2941 gaagaagcag ttcgtgaata caaaaaaggc gacgaaatcg ctgcagttgt tctgcaggtt
     3001 gacgcagaac gtgaacgtat ctccctgggc gttaaacagc tcgcagaaga tccgttcaac
     3061 aactgggttg ctctgaacaa gaaaggcgct atcgtaaccg gtaaagtaac tgcagttgac
     3121 gctaaaggcg caaccgtaga actggctgac ggcgttgaag gttacctgcg tgcttctgaa
     3181 gcatcccgtg accgcgttga agacgctacc ctggttctga gcgttggcga cgaagttgaa
     3241 gctaaattca ccggcgttga tcgtaaaaac cgcgcaatca gcctgtctgt tcgtgcgaaa
     3301 gacgaagctg acgagaaaga tgcaatcgca actgttaaca aacaggaaga tgcaaacttc
     3361 tccaacaacg caatggctga agctttcaaa gcagctaaag gcgagtaatt ctctgactct
     3421 tcgggatttt tattccgaag tttgttgagt ttacttgaca gattgcaggt ttcgtcctgt
     3481 aatcaagcac taagggcggc tacggccgcc cttaatcaat gcagcaacag cagccgctta
     3541 atttgccttt aaggaaccgg aggaatcatg accaagtcag aattgataga aagacttgcc
     3601 acccagcaat cgcacatttc cgccaagacg gttgaagatg cagtaaaaga gatgctggag
     3661 catatggcct cgactcttgc gcagggcgag cgtattgaaa tccgcggttt cggcagtttc
     3721 tctttgcact accgcgcacc acgtaccgga cgtaatccga agactggcga taaagtagaa
     3781 ctggaaggaa aatacgttcc tcactttaaa cctggtaaag aactgcgcga tcgcgccaat
     3841 atttacggtt aagtttttac tcaaacttga acgcgagaaa agcacctgtc gggtgctttt
     3901 ttcatttctc taatcaggaa ctggaagctg cctcgcagag ttttgagcag ttttcaccct
     3961 ttcgttaaat tcttctgaat gtgcctcggg gaacgcaaaa ttcccaccag acaaccgctc
     4021 aacaaagttg cacactttcc ataaacaggg aggggtgcat gaaaataacg acagtcggcg
     4081 tatgcataat ttgcggaatt tttccgttgc tgattttgcc ccaattgcct gggacattaa
     4141 cccttgcgtt tctgactctc ttcgcctgcg tactggcatt tatccctgtt aaaaccgtcc
     4201 gttatatcgc gctgacgttg ctgtttttcg tttggggcat attatcagca aagcaaattt
     4261 tgtgggcagg agaaacctta actggcgcga cgcaggatgc aattgttgag atcactgcaa
     4321 ctgacggcat gaccactcat tacggtcaaa ttactcatct acaaggtcga cgtatattcc
     4381 ctgcgccagg ccttgtgctg tatggcgaat atcttccgca agcggtttgt gctggacaac
     4441 aatggtcaat gaaactcaaa gttcgtgcgg ttcatggcca acttaatgat ggcggctttg
     4501 atagccagcg ttatgccatt gcccagcatc aaccgctcac cggccgcttt ctgcaggcaa
     4561 gtgttattga accgaattgt agcctgcgtg cacagtatct ggcgtcacta caaacaacgc
     4621 tgcaacccta tccgtggaat gcggttattc ttggtttagg tatgggggaa cggttatccg
     4681 tccccaaaga aatcaaaaat atcatgcgcg atactggaac ggcgcattta atggcgatat
     4741 cgggattgca catcgctttt gcggcgttgc tggctgccgg actcattcgc agtggacaaa
     4801 tttttctgcc tgggcgctgg atccactggc aaataccatt aattggtggg atctgctgtg
     4861 ctgcttttta tgcctggttg acgggaatgc aacctcctgc attgcgtacc gtggtggcgc
     4921 ttgctacgtg gggaatgctt aagttaagtg ggcgacagtg gagtggctgg gatgtatgga
     4981 tatgttgtct ggcggcaatt ttgctgatgg atcctgttgc cattctctcg caaagtttat
     5041 ggctctctgc cgctgcggtc gcggcactga ttttttggta tcagtggttt ccctgtcctg
     5101 agtggcaact gccgccggta ttgcgtgcag ttgtttccct catccatctg caactgggta
     5161 tcacacttct gcttatgccc gtgcaaatcg tcatatttca tggcattagt ctgacctcgt
     5221 ttattgcaaa tctattagca attcccttgg tgacatttat cacggttccg ttgatcctcg
     5281 ccgcgatggt tgtgcattta agcgggccgt taatcctgga gcaagggtta tggtttcttg
     5341 ccgaccggtc tttggcttta cttttctggg ggttaaagag tttgccagaa gggtggatca
     5401 acattgctga acgttggcaa tggctatcat tttccccatg gttcttactg gtggtatggc
     5461 gattaaacgc ctggcgaacg ttgccagcaa tgtgtgtggc tgtaggcttg ctgatgtgct
     5521 ggccgctatg gcaaaaacct cgacctgacg agtggcaggt gtacatgctt gatgtcgggc
     5581 aagggctggc aatggtgata gccagaaacg gcaaagcgat tctctatgac acaggactgg
     5641 cctggcccga aggggatagt gggcaacaac tgattatccc ctggctccac tggcataatc
     5701 ttgaaccgga aggcgttatt ctgagtcatg aacatctgga tcaccgggga gggctggact
     5761 caatattgca cacatggccg atgttatgga tcagaagtcc gttaaactgg gaacatcatc
     5821 agccctgtgt gcgtggcgaa gcgtggcaat ggcaaggatt gcgtttcagc gtgcactggc
     5881 ctttacaagc tagcaacgat aaaggaaata accattcctg tgtggttaag gttgatgacg
     5941 ggacgaatag cattcttcta accggtgata ttgaagtccc cgctgaacaa aagatgctaa
     6001 gccgttactg gcagcaagtg cagacaacat tgcttcaggt acctcaccat ggcagtaata
     6061 cctcatcatc gttgccatta attcagcgag tgaatggaaa agtggcactc gcatcggcat
     6121 cgcgctataa cgcatggcga ttgccctcta ataaagttaa gcatcgctat caacagcaag
     6181 gatatcaatg gcttgatact ccacatcagg gtcaagtgac ggtcaatttt tcagcgcaag
     6241 gctggcggat tagcagcctc agagagcaaa ttttacctcg ttggtatcat cagtggtttg
     6301 gcgtgccagt ggataacggg tagaatatgc ggctatttca acaaatgctg gttttttgaa
     6361 tgcataacga caaagatctc tctacgtggc agacgttccg ccgactgtgg ccaaccattg
     6421 cgccttttaa agcgggtctg atcgtggcgg gcgtagcgtt aatcctcaac gcagccagcg
     6481 ataccttcat gttatcgctc cttaagccac ttcttgatga tggctttggt aaaacagatc
     6541 gctccgtgct ggtgtggatg ccgctggtgg tgatcgggct gatgatttta cgtggtatca
     6601 ccagctatgt ctccagctac tgtatctcct gggtatcagg aaaggtggta atgaccatgc
     6661 gtcgccgcct gtttggtcac atgatgggaa tgccggtttc attttttgac aaacagtcaa
     6721 cggggacgct gttgtcacgt atcacctacg attccgaaca ggttgcttct tcttcttccg
     6781 gcgcactgat aacggttgtg cgtgaaggtg cgtcaatcat cggcctgttc atcatgatgt
     6841 tctattacag ttggcaactg tcgatcattt tgattgtgct ggcaccgatt gtttcgattg
     6901 cgattcgcgt agtatcgaag cgttttcgca acatcagtaa aaacatgcag aacaccatgg
     6961 ggcaggtgac caccagcgcc gaacaaatgc tgaagggcca caaagaagta ttgattttcg
     7021 gtggtcagga agtggaaacg aaacgctttg ataaagtcag caaccgaatg cgtcttcagg
     7081 ggatgaaaat ggtttcagcc tcttccatct ctgatccgat cattcagctg atcgcctctt
     7141 tggcgctggc gtttgttctg tatgcggcga gcttcccaag tgtcatggat agcctgactg
     7201 ccggtacgat taccgttgtt ttctcttcaa tgattgcact gatgcgtccg ctgaaatcac
     7261 tgaccaacgt taacgcccag ttccagcgcg gtatggcggc ttgtcagacg ctgtttacca
     7321 ttctggacag tgagcaggag aaagacgaag gtaagcgcgt gatcgagcgt gcgactggcg
     7381 acgtggaatt ccgcaatgtc acctttactt atccgggacg tgacgtacct gcattgcgta
     7441 acatcaacct gaaaattccg gcagggaaga cggttgctct ggttggacgc tctggttcag
     7501 gtaaatcaac catcgccagc ctgatcactc gtttttacga tattgatgaa ggcgaaatcc
     7561 tgatggatgg tcacgatctg cgcgagtata ccctggcgtc gttacgtaac caggttgctc
     7621 tggtgtcgca gaatgtccat ctgtttaacg atacggttgc taacaacatt gcttacgcac
     7681 ggactgaaca gtacagccgt gagcaaattg aagaagcggc gcgtatggcc tacgccatgg
     7741 acttcatcaa taagatggat aacggtctcg atacagtgat tggtgaaaac ggcgtgctgc
     7801 tctctggcgg tcagcgtcag cgtattgcta tcgctcgagc cttgctgcgt gatagcccga
     7861 ttctgattct ggatgaagct acctcggctc tggataccga atccgaacgt gcgattcagg
     7921 cggcactgga tgagttgcag aaaaaccgta cctctctggt gattgcccac cgcttgtcta
     7981 ccattgaaaa ggcagacgaa atcgtggtcg tcgaggatgg tgtcattgtg gaacgcggta
     8041 cgcataacga tttgcttgag caccgcggcg tttacgcgca acttcacaaa atgcagtttg
     8101 gccaatgatc gaaaaaatct ggtctggtga atcccctttg tggcggctat tgctgccact
     8161 ctcctggttg tatggcctgg tgagtggcgc gatccgtctt tgctataaac taaaactgaa
     8221 gcgcgcctgg cgtgcccccg taccggttgt cgtggttggt aatctcaccg caggcggcaa
     8281 cggaaaaacc ccggtcgttg tctggctggt ggaacagttg caacagcgcg gaattcgagt
     8341 gggggtcgta tcgcggggat atggtggtaa ggctgaatct tatccgctgt tattgtcggc
     8401 agataccaca acagcacagg cgggtgatga acctgtgttg atttatcaac gcactgatgc
     8461 gcctgttgcg gtttctcccg ttcgttctga tgcggtaaaa gccattctgg cgcaacaccc
     8521 tgatgtgcag atcatcgtaa ccgacgacgg tttacagcat taccgtctgg cgcgtgatgt
     8581 ggaaattgtc gttattgatg gtgtgcgtcg ctttggcaat ggctggtggt tgccggcggg
     8641 gccaatgcgt gagcgagcgg ggcgcttaaa gtcagttgat gcggtaatcg tcaacggcgg
     8701 tgtcccccgc agcggtgaaa tccccatgca tctgctgccg ggtcaggcgg tgaatttacg
     8761 taccggtacg cgttgtgatg ttgctcagct tgaacatgtg gtggcgatgg cagggattgg
     8821 gcatccgccg cgcttttttg ccacgctgaa gatgtgcggc gtacaaccgg aaaaatgtgt
     8881 accgctggcc gatcatcagt ctttgaacca tgcggatgtc agcgcgttgg taagcaccgg
     8941 gcaaacgctg gtaatgactg aaaaagatgc ggtgaaatgc cgggcctttg cagaagaaaa
     9001 ttggtggtat ttgcctgtag acgcacagct ttcaggtgat gaaccagcga aactgcttac
     9061 gcaactaacc tcgctggctt ctggcaacta gttacgccgc ggcagcgttc gattgatgga
     9121 gtcatgaatg tcgctgccgc acctctccct tgctgatgcg cgtaatcttc accttgccgc
     9181 acaaggcctg ttaaacaaac cccgccgtcg agcgtcgttg gaggatattc cggcaacgat
     9241 ctcccgcatg tccttgctgc aaatcgatac catcaatatt gttgcccgta gtccatatct
     9301 ggtgcttttc agtcgtctgg gaaattatcc tgcccagtgg ctggatgagt ctctggcgcg
     9361 tggcgaatta atggaatact gggcgcatga agcctgcttt atgccacgca gcgactttcg
     9421 tcttattcgc caccgcatgc tggcacctga aaaaatgggc tggaaataca aagacgcctg
     9481 gatgcaggaa catgaggcgg aaattgcaca gttaattcag catattcatg ataaggggcc
     9541 ggtacgttca gccgattttg agcatcctcg taaaggtgca agcggctggt gggaatggaa
     9601 gccgcataaa cggcatctgg aaggtttatt tactgccgga aaggtgatgg tgattgaacg
     9661 gcgcaacttc cagcgcgttt atgatttaac ccaccgtgtc atgcctgact gggatgatga
     9721 gcgcgatctc gtttcgcaaa cagaagcaga aatcatcatg ctggataaca gtgcgcgtag
     9781 cctgggaata ttccgcgaac agtggctggc agattactat cggctgaaac gtccggcact
     9841 ggcggcgtgg cgcgaagcga gggctgaaca gcagcaaatc attgctgtgc atgttgaaaa
     9901 attgggcaat ctttggctgc atgctgattt gctgccgcta ctcgagcgtg cgctggccgg
     9961 aaagctcact gcaacgcaca gcgcggtact ttcgcctttt gatcctgttg tctgggatcg
    10021 caaacgcgca gagcagcttt ttgattttag ctaccggctg gagtgctata ccccagcgcc
    10081 gaaacgccag tatggctatt ttgttctgcc attattacat cgtgggcaat tagttgggcg
    10141 aatggatgcc aaaatgcatc gccagacagg catccttgaa gttatctctc tgtggttaca
    10201 ggaaggcatt aaaccaacga caatgctgca aaaagggtta cgtcaggcga ttactgattt
    10261 cgcgagctgg cagcaggcaa cgcgggtgac attaggacgc tgcccgcaag gtctctttac
    10321 tgattgccgc actggctggg aaatagaccc cgtcgcataa atgattatga taagatttat
    10381 gcattcataa cccgatccat ctggaggaac tatggatcat cgtctgcttg aaatcattgc
    10441 ctgcccggtt tgcaacggaa aactttggta taaccaagaa aaacaagaac ttatttgcaa
    10501 actggacaac ctggctttcc cgctgcgtga tggcattcca gtgttgctgg aaacggaagc
    10561 ccgcgtgctg actgctgatg agagtaaatc atgagttttg tggtcattat tcccgcgcgc
    10621 tacgcgtcaa cgcgtctgcc cggtaaacca ttggttgata ttaatggcaa acccatgatt
    10681 gttcatgttc ttgaacgtgc tcgcgaatca ggtgccgatc gcatcatcgt ggcaaccgat
    10741 catgaggatg tcgcccgcgc cgttgaagcc gctggcggtg aagtatgtat gacgcgcgcc
    10801 gatcaccagt cagggacaga acgcctggcg gaagttgtcg aaaaatgcgc attcagcgac
    10861 gacacggtga tcgttaatgt gcagggtgat gaaccgatga tccctgcgac aatcattcgt
    10921 caggttgctg ataacctcgc tcagcgtcag gtgggtatgg cgactctggc ggtgccaatc
    10981 cacaatgcgg aagaagcgtt taacccgaat gcggtgaaag tggttctcga cgctgaaggg
    11041 tatgcactgt acttctctcg cgccaccatt ccttgggatc gtgatcgttt tgcagaaggc
    11101 cttgaaaccg ttggcgataa cttcctgcgt catcttggta tttatggcta ccgtgcaggc
    11161 tttatccgtc gttacgtcaa ctggcagcca agtccgttag aacacatcga aatgttagag
    11221 cagcttcgtg ttctgtggta cggcgaaaaa atccatgttg ctgttgctca ggaagttcct
    11281 ggcacaggtg tggatacccc tgaagatctt gagcgcgttc gcgctgaaat gcgctaattt
    11341 cacttcacga cacttcagcc aattttggga ggagtgtcgt accgttacga ttttcctcaa
    11401 tttttctttt caacaattga tctcattcag gtgacatctt ttatattggc gctcattatg
    11461 aaagcagtag cttttatgag ggtaatctga atggaacagc tgcgtgccga attaagccat
    11521 ttactgggcg aaaaactcag tcgtattgag tgcgtcaatg aaaaagcgga tacggcgttg
    11581 tgggctttgt atgacagcca gggaaaccca atgccgttaa tggcaagaag ctttagtacg
    11641 cccggaaaag cccgacaact ggcatggaaa accaccatgc tggcaagaag tgggactgtc
    11701 cgtatgccga ctatttatgg tgtgatgacg catgaagaac accccggccc cgatgtcctg
    11761 ctactggagc ggatgcgtgg tgtttcggtg gaggcaccag cccgaacacc agaacgctgg
    11821 gaacaactca aagaccaaat cgttgaagcc ttactggcct ggcaccgtca ggacagtcgc
    11881 ggttgcgtcg gcgcggtcga caatactcag gaaaatttct ggccctcatg gtaccggcaa
    11941 catgttgaag tgctatggac cacgctcaat cagttcaata acaccggtct gacgatgcag
    12001 gataagcgga tcctgtttcg cactcgcgaa tgtctcccgg cattatttga aggctttaac
    12061 gacaattgtg tgttgattca cggtaacttc tgtttacgca gcatgctgaa agattcgcgc
    12121 agcgatcagt tactggcgat ggtcgggccg ggactaatgc tttgggcacc gcgagaatac
    12181 gaactgttcc gactaatgga taattctctg gcggaagatt tgctctggag ttacctgcaa
    12241 cgcgcgccag tggcggagtc gttcatctgg cggcgttggt tgtatgtgtt atgggatgaa
    12301 gttgcgcaac tggttaatac cggacgattt agtcggcgca acttcgatct ggcatcaaaa
    12361 tcactcttgc cgtggctcgc ctgacggacc tttcagccac tgccagatgc ggccaagcgt
    12421 ttcatagcca acacgatcgc tatgcatcag ccagaccgga gaagggattg cccgttccca
    12481 cggattaagc ggcgagtcga tggccaactg attcgccggg gctggtagcg gatttaaccc
    12541 ttcctgctga aaaaagatca tcgcccgcgg cagatgtgat gcggaggtca ccagcaggaa
    12601 aggggcgtca ccaatcgcct gtttcactgc cgcagcttct tcttcggtat cttttggcaa
    12661 atccagggtg ataatttgct cgcgcggcac gcccagcgat tgcgcaactc tggcacctac
    12721 ttccgctgta ctcaccgtat tggtttttgc tacgcctccc gtgaagatca gttttgatcc
    12781 cggattttcg cgccataagc gaataccttc attcaggcgt ggcagactgt tattgattaa
    12841 attagagctc ggtgcccact gcgggttcca ggtataacca cctccgagca ccacgatata
    12901 gtccactttt tgcgaattat tccatgtcgg ataggtgctt tcgatgggac gcagcaggcg
    12961 gtcggctacc ggttgcaaac tcaataacaa gagtgccagc cacccaatgc taataaaaat
    13021 cttcccggtt ttttgaaaac ggctaaacca cagcagcgcc aggccagcgc caataattaa
    13081 caacatcagc ggaagtggca gcaacatatt gccaatcact tttttcagtg taaaaagcat
    13141 cctttctggt tcctttttta accatatagc aagggatcgc cagcgatatt acaccagacg
    13201 ggttcattct cgccgtggct gtgacaaaat agcgtcttta tcagaaaacg tcttagccaa
    13261 ccggtggaga aagcaatgca ggatcgcaat tttgatgata ttgcggaaaa gttttcccgt
    13321 aacatttacg gcaccaccaa agggcagctt cgacaggcta ttctgtggca ggatctcgat
    13381 cgcgtgctgg cggaaatggg cccgcaaaaa ctgcgtgtgc tggatgctgg cggtggagaa
    13441 gggcagaccg caatcaaaat ggccgagcgt gggcatcagg tcattttatg cgatctttct
    13501 gcgcagatga tcgaccgcgc aaaacaggcg gcagaagcaa aaggtgtgag cgacaacatg
    13561 caatttatac attgcgccgc tcaggatgtt gcttcgcatt tggaaacgcc cgttgatctg
    13621 atattgttcc atgcggtgct cgagtgggtg gccgatcccc gcagtgtatt gcagaccctg
    13681 tggtcagtgt tgcgtccagg cggcgtgttg tcgttaatgt tctacaatgc gcatggtttg
    13741 ttgatgcata acatggtcgc cgggaatttt gattacgtgc aggcgggaat gccgaaaaag
    13801 aaaaaacgga cgctttcgcc agattatcca cgcgacccgg cgcaggttta tctgtggctg
    13861 gaagaagctg gttggcaaat tatgggtaag acaggcgttc gcgtgtttca tgattatctg
    13921 cgcgagaagc accagcagcg cgactgctat gaagcattac ttgaattaga aacgcgttat
    13981 tgccgtcagg aaccgtatat taccctgggg cgttatattc atgtcaccgc gcgcaaaccg
    14041 cagagcaagg ataaagtatg agtgaatttt cccagacagt ccccgaactg gttgcctggg
    14101 ccagaaaaaa tgacttctcc atctcgctgc cggtagaccg actctctttt ctgctggcgg
    14161 ttgccacgct gaacggcgag cgtctggatg gtgagatgag tgaaggcgag ctggtggatg
    14221 cattccgcca tgtgagtgat gcgtttgagc aaaccagcga aaccatcggc gtgcgcgcca
    14281 ataacgcgat caacgacatg gtgcgtcaac gtctgctgaa ccgctttacc agcgagcagg
    14341 cggaagggaa cgcaatttac cgtctgacgc cgctcggcat cggcatcact gactattaca
    14401 ttcgtcagcg cgagttttct acgctgcgcc tttctatgca gttgtcgatt gtggcgggtg
    14461 agctcaaacg cgcagcagat gccgccgaag agggcggtga tgaatttcac tggcaccgta
    14521 atgtttatgc tccactgaaa tattcggtag cagaaatttt cgacagtatc gacctgacgc
    14581 aacgtctgat ggatgaacag cagcagcagg tgaaggatga tatcgcccag ttgctgaaca
    14641 aagactggcg ggcggcgatt tccagctgtg aattgttgct ttcggaaact tccggaacgc
    14701 tgcgtgaatt gcaggatacg ctggaagcgg caggcgacaa attgcaggct aatctgttgc
    14761 gcattcagga tgcgacgatg acccatgacg atctgcattt cgtcgatcgt ctggtgttcg
    14821 atctgcagag caaactcgat cgtattatca gttggggcca gcaatccatc gacttgtgga
    14881 ttggctacga ccgccacgta cacaaattta ttcgtaccgc gatcgatatg gataaaaacc
    14941 gcgtctttgc tcagcggtta cgtcagtcgg tacaaaccta ttttgatgag ccgtgggcgc
    15001 taacttatgc caatgccgat cgtctgctgg atatgcgtga cgaagagatg gcactgcgcg
    15061 atgaagaagt gactggggaa cttcctgagg atctggaata cgaagagttt aacgagatcc
    15121 gcgaacagct ggcggcgatc atcgaagaac aacttgccgt gtacaaaacc agacaagtgc
    15181 cgctggatct tggtctggtg gtacgcgaat atctgtcaca gtatccgcgt gcacgtcact
    15241 ttgacgttgc gcgcattgtt attgatcagg cggtacgtct tggcgtagcg caagcagatt
    15301 tcaccggact gccagcgaaa tggcagccga ttaatgatta cggagccaag gtacaggcgc
    15361 atgtcatcga caaatattga acaagtgatg ccggttaagc tggcgcaggc gctggcgaat
    15421 ccgttatttc cggcgctgga cagcgcctta cgttcaggcc gccatattgg cctcgacgaa
    15481 ctggataatc atgcattcct gatggatttt caggaatatc tggaagagtt ttacgcgcgt
    15541 tataacgttg agcttattcg cgcaccagaa gggttcttct atttacgccc acgttccacc
    15601 acgctgatcc ctcgttccgt cttgtcggaa ctggatatga tggtcgggaa aatcctctgt
    15661 tatctctatc tcagcccgga acggctggcg aatgagggga ttttcaccca gcaggaactg
    15721 tacgacgaac tgctcaccct ggccgatgaa gcaaaactgc tgaaactggt gaacaaccgt
    15781 tcaaccggtt cagacgttga ccgtcagaag ttgcaggaga aagtacgttc ttcgctcaac
    15841 cgtctgcgtc gtttaggcat ggtgtggttt atgggccacg acagcagcaa gtttcgcatt
    15901 accgaatcgg tgttccgctt cggagccgat gtgcgtgctg gcgacgatcc ccgtgaagca
    15961 cagcgtcgcc tgattcgtga tggcgaagca atgccgattg aaaatcatct gcaactcaac
    16021 gatgaaaccg aagagaatca gccagatagc ggagaggaag aataatgatt gaacgcggta
    16081 aatttcgctc actgacgctg attaactgga acggcttttt tgcccgaact tttgaccttg
    16141 acgagctggt cacgacgctt tctggcggta acggggcggg taaatccacc accatggcgg
    16201 cgttcgttac ggcgctgatc cccgacctga ccctgctgca tttccgtaac actacggaag
    16261 ccggggccac cagcggttcg cgcgataaag gtctgcacgg taagctgaaa gcgggtgtct
    16321 gttattcgat gctcgacacc attaactcgc gccaccagcg cgtggtggtc ggtgtgcgtc
    16381 tgcaacaggt tgccggacgc gatcgtaaag tggatatcaa gccgtttgcc attcagggac
    16441 tgccgatgtc ggtgcagccg acacagctgg tgaccgaaac cctgaacgaa cgccaggcgc
    16501 gcgtgctgcc gcttaacgag ctgaaagaca agctcgaggc gatggaaggc gtgcagttta
    16561 aacagttcaa ctccattact gattaccact cgctgatgtt cgatctgggc atcatcgcgc
    16621 gtcgtctgcg ctccgcatct gaccgtagca aattctatcg tctgatcgaa gcttcgctgt
    16681 atggcgggat ctccagtgcc attacccgtt ctctgcgcga ctacctgttg ccggaaaaca
    16741 gcggcgtgcg taaagcgttc caggacatgg aagcggcgct gcgtgaaaac cgtatgacgc
    16801 tggaagcgat tcgtgtcacc cagtcggacc gcgacctgtt taagcatctg atcagcgaag
    16861 ccaccaacta cgtggcggcg gactacatgc gtcacgccaa cgagcgccgt gtccatctcg
    16921 acaaagccct ggagtttcgt cgcgagctac atacttcgcg tcagcaactg gcggctgagc
    16981 agtacaaaca cgtcgatatg gcgcgtgagc tggcagagca caacggtgcc gaaggcgatc
    17041 tggaagcgga ttatcaggcg gccagcgatc acctgaacct ggtgcaaacc gcactgcgtc
    17101 agcaggagaa gatcgaacgc tacgaagcgg atctcgatga gctgcagatc cgtctggaag
    17161 agcaaaatga agtggtggca gaagccatcg aacgccagga agagaatgag gctcgtgcgg
    17221 aagctgccga actggaagtg gacgagctga aaagccagct tgctgactac cagcaggcgc
    17281 tggacgtcca gcaaacgcgc gcgatccagt ataaccaggc gattgctgcg cttaatcgtg
    17341 ccaaagaact gtgccatctg ccggacttaa ccgccgactg cgccgccgaa tggctggaaa
    17401 ccttccaggc gaaagagctg gaagcgactg aaaaaatgct ctctcttgag cagaaaatga
    17461 gcatggcgca aaccgcgcac agccagtttg agcaggctta tcagctggtg gtggcaatca
    17521 acggcccgct ggcgcgtaac gaggcgtggg atgtcgctcg cgaactattg cgcgaagggg
    17581 tcgatcagcg tcacctggca gagcaggttc aaccgttgcg gatgcgctta agtgaactgg
    17641 aacagcgtct gcgcgagcag caagaagccg aacgcctgct ggcagacttc tgcaaacgtc
    17701 agggcaagaa ttttgatatt gatgaactgg aagctctgca tcaggagctg gaagcgcgca
    17761 ttgcctctct ttccgatagc gtgtctaacg cccgtgaaga gcgcatggca ctgcgccagg
    17821 aacaggaaca gctgcagtct cgcattcaga gtttgatgca gcgtgcgccg gtttggctgg
    17881 cagcgcaaaa cagtctcaac cagttgagcg agcagtgcgg tgaagagttt acctccagcc
    17941 aggacgtcac cgaatatctg caacagttgc tggagcgtga gcgagaggcg attgttgaac
    18001 gcgatgaagt gggcgcgcgc aaaaacgctg tcgatgaaga gatcgaacgt ttaagccagc
    18061 ctggcggctc tgaagatcag cgtctgaacg cgctggcgga gcgttttggt ggtgtgctgc
    18121 tgtcagaaat ttatgacgac gttagcctgg aagatgcgcc gtacttctca gcgctgtatg
    18181 gcccgtcacg ccacgccatc gtggtgccag atctgtcaca ggtaactgaa cacctggaag
    18241 gcttgaccga ttgcccggaa gatctctatc tgatcgaagg agatccgcag tcattcgatg
    18301 acagcgtgtt cagcgttgat gagctggaaa aagcggtagt ggtgaaaatc gccgatcgtc
    18361 agtggcgtta ttcacgtttc ccggaagtgc cgctgtttgg tcgtgctgcg cgtgaaagcc
    18421 gtattgaaag cctccatgcc gagcgtgaag tgctttccga acgcttcgcc acgctctcct
    18481 ttgatgtaca gaaaactcag cgtctgcatc aggcgttcag ccgctttatc ggcagtcatc
    18541 tggcggttgc gtttgagtct gacccggaag cagaaatccg tcaactgaac agccgtcgcg
    18601 tcgaactgga gcgggcgtta agtaatcatg aaaatgataa ccagcagcag cgtattcagt
    18661 ttgagcaggc gaaagagggc gttacggcgc tgaaccgcat tctgccgcgt ctcaacctgt
    18721 tggctgatga cagtctggcg gatcgcgtcg atgaaatccg cgaacgtctg gatgaagccc
    18781 aggaagccgc gcgttttgtt cagcagtttg gcaatcaact ggcgaaactg gaaccgattg
    18841 tttcggtatt gcagagcgac ccggaacagt tcgaacagtt aaaagaagat tacgcgtact
    18901 ctcagcagat gcagcgcgat gcccgtcagc aggcgtttgc cctgacggaa gtggtgcagc
    18961 gtcgtgcgca ctttagctat tctgactcgg cagaaatgct tagcggtaac agcgatctca
    19021 acgaaaaact gcgtgaacgt ctggaacagg cggaagcgga gcgtacccgc gctcgcgaag
    19081 cgttgcgcgg tcacgcagcg cagttgagtc agtacaacca ggtgctggct tcgctgaaaa
    19141 gttcttacga caccaaaaaa gagctactca acgatctgca acgtgaattg caggatatcg
    19201 gcgtgcgtgc tgatagcggg gcagaagagc gggcgcgtat tcgccgtgac gagctgcatg
    19261 cgcaactgag caataaccgt tcacgccgca atcaactgga aaaagcgctt accttctgcg
    19321 aagcggagat ggacaacctg acccgcaaac tgcgcaagct ggagcgggat tactttgaga
    19381 tgcgcgagca ggtagtgacc gccaaagcgg gctggtgtgc ggtgatgcgc atggtgaaag
    19441 ataacggcgt tgagcgccgc ttacaccgtc gtgagctggc ttatctctcc gctgatgatt
    19501 tgcgttccat gtcggataag gcgttaggtg cgctgcgtct ggcggtggcg gataacgaac
    19561 atctgcgcga cgtgctgcgc atgtcggaag atccgaaacg tccggagcgt aaaattcagt
    19621 tcttcgtggc ggtttatcag catctgtgtg aacgtattcg tcaggatatt attcgtaccg
    19681 atgatccggt ggaagctatc gaacagatgg agattgaact tagccgtctg accgaagaat
    19741 taacctcccg tgaacagaaa ctggcgatca gttcccgcag cgtggcgaac atcattcgca
    19801 aaaccattca gcgcgagcag aaccgtatcc gtatgctcaa ccaggggttg cagaacgtat
    19861 cgtttggtca ggtgaacagc gtgcgtctca acgtgaacgt gcgtgaaacg cacgccatgc
    19921 tactggatgt gctctctgaa cagcacgagc agcatcagga tctgtttaac agcaaccgtt
    19981 tgaccttctc ggaagcgctg gcgaaactgt atcaacgtct taacccgcag attgatatgg
    20041 ggcagcgcac gccgcagacc atcggtgaag aactgctgga ttaccgcaac tatctggaaa
    20101 tggaagttga ggttaaccgt ggttccgatg gctggctgcg cgcagagtct ggtgcattgt
    20161 cgaccggtga ggcgattggt accggtatgt cgattctggt gatggtggta caaagctggg
    20221 aagatgaatc tcgccgcctg cgcggtaaag atatctctcc ttgccgcctg ctgttcctcg
    20281 atgaagcagc gcgactggat gctcgttcta tcgccacgct gtttgaattg tgtgaacgtt
    20341 tgcaaatgca gctcatcatc gcagcgccgg aaaatatcag cccggagaaa ggcaccacct
    20401 ataaactggt gcgtaaagtc ttccagaata ccgaacacgt tcacgtcgtc ggcctgcgag
    20461 gatttgcgcc gcaactccct gaaacgcttc caggaagtga cgaagcgcct tctcaggcga
    20521 gttaaaatta aggcggcagc aatgccgcct tttctttttc cgaaaactcc gtttctgcac
    20581 taaaaaagtg gcacattacg gcgcgctttt ctttaaactt ctttacatta ggttatgtaa
    20641 aaacgtatcg gcgtttatat actgaagata agcctgatga gtaacaggct tgctcgtcat
    20701 actttcgtga gtattggcgt tgtacaggca agtcgtaaaa taacagcctg gctattcaga
    20761 gtatgataaa aacagggggc aagggatgtt gcttaatatg atgtgtggtc gtcggctgtc
    20821 ggcaatcagt ttgtgcctgg ccgtaacatt cgctccactg ttcaatgcgc aggccgatga
    20881 gcctgaagta atccctggcg acagcccggt ggctgtcagt gaacagggcg aggcactgcc
    20941 gcaggcgcaa gccacggcaa taatggcggg gatccagcca ttgcctgaag gtgcggcaga
    21001 aaaagcccgc acgcaaatcg aatctcaatt acccgcaggt tacaagccgg tttatcttaa
    21061 ccagcttcaa ctgttgtatg ccgcacgcga tatgcaaccc atgtgggaaa accgtgatgc
    21121 tgttaaagcc ttccagcaac agctggcaga ggtggcgatt gccggtttcc agccgcagtt
    21181 taataaatgg gtagagttac tgaccgatcc tggtgttaac gggatggcac gcgacgtagt
    21241 gctctctgat gcgatgatgg gctatctcca tttcattgca aatattccgg tcaaaggcac
    21301 tcgctggcta tatagcagta aaccttatgc acttgcaacg ccgccgctct cggtgattaa
    21361 ccaatggcag ctggcgctgg ataaaggtca attgcctacg tttgttgctg gactggcacc
    21421 gcagcatccg caatatgcgg cgatgcatga atcgttactg gccttactca gtgacaccaa
    21481 accgtggccc caactgaccg gcaaagcaac gttgcgccca gggcagtgga gtaacgacgt
    21541 accggcgttg cgcgaaatat tgcaacgcac aggcatgttg gacggggggc cgaaaattac
    21601 tctacctggc gatgacacgc caactgacgc ggtagtcagc ccatccgctg ttactgttga
    21661 aacagcagaa actaagccga tggacaagca aacgacatct cgtagtaaac ctgcgcctgc
    21721 cgttcgcgcc gcctacgata atgaactggt ggaagccgtt aaacgttttc aggcatggca
    21781 aggattgggg gcagatggtg ctattggccc ggcaacacgt gactggttaa acgtaacgcc
    21841 cgcccagcgt gctggcgtgt tggctctcaa catccagcga ttgcgcttgc tgccaacaga
    21901 gctttctacc gggatcatgg ttaacattcc ggcctattcg ctggtctact atcagaacgg
    21961 caatcaggtg ctggattcgc gagtcattgt cggtcgcccc gatcgcaaaa cgccgatgat
    22021 gagcagtgcc cttaacaacg tagtggtaaa cccgccgtgg aacgtacctc caactctggc
    22081 acgcaaagat attctgccaa aagtgcgcaa cgatccggga tatctcgaaa gccatggcta
    22141 tacggtgatg cgcggctgga acagcagaga agcgattgac ccatggcagg ttgactggtc
    22201 tacaatcacg gcctcgaatt taccgttccg cttccagcag gctccaggcc cacggaactc
    22261 gctggggcgc tataaattca atatgccgag ttcagaggcc atttatttgc atgacacgcc
    22321 gaaccacaat ctgttcaagc gtgatacacg cgcattgagc tcaggctgtg tacgagtgaa
    22381 taaagcttcc gatctggcga atatgctgtt gcaggatgca ggctggaatg acaaacgtat
    22441 ttctgatgcg ctgaagcagg gtgatacacg ttacgtcaat attcggcagt cgattccggt
    22501 gaatctctac tacctgacgg cctttgttgg tgcagatggt cgtacccagt atcgtacaga
    22561 tatttacaat tatgatctgc ctgcgcgatc cagctcgcaa atcgtatcga aagcggaaca
    22621 attaatcagg taaatgaagt agttcgggga aatcagttgt cgtaataatt agcatgattg
    22681 ggggcgattc tctgcagccc ccgtcactgc tggggttgag tcatcttgac gtctgcttta
    22741 cgggcggtta aggtgcctct tgtgcgccag aagtgcatat aaacgataac attgacctgt
    22801 agacttgatt atcatggaca aattcgacgc taatcgccgc aaattgctgg cgcttggtgg
    22861 cgttgcactc ggtgccgcca tcctgccgac ccctgcgttt gcaacactct ctaccccacg
    22921 cccgcgcatt ttgacactca ataatcttca taccggagag tcaatcaaag cggagttttt
    22981 cgatggcaga ggctatattc aggaagaatt ggcaaaactt aaccattttt tccgcgatta
    23041 ccgcgcgaac aaaataaagt ccatcgaccc aggattattc gaccagttgt atcgcctgca
    23101 agggttgtta ggcacgcgca aaccggtgca actcatttcc ggttatcgtt ctattgatac
    23161 caacaatgaa ctacgcgccc gcagccctgg agtagcgaag aaaagctatc acactaaagg
    23221 ccaggcgatg gatttccata ttgaaggtat cgcgttaagc aatattcgca aagccgcgtt
    23281 atctatgcgc gcaggtggtg taggatatta tccacgtagt aactttgtgc atattgatac
    23341 cgggccagca cggcactggt agtaattgct taacgaaaca ggggcagtat gaactatcgt
    23401 attattccgg tcaccgcatt ctcccagaac tgttcattaa tctggtgtga acaaacccgt
    23461 ctggccgcac tggtcgatcc tggcggcgat gcggaaaaaa tcaaacagga agttgatgac
    23521 agcggcctga cactgatgca gatcctgctg acgcatggtc atctggacca cgttggcgca
    23581 gcggcggaac tggcgcaaca ttacggcgtg ccggtttttg gcccggaaaa agaagatgag
    23641 ttctggctgc aaggcttgcc tgcgcaaagt cgtatgtttg gtctggaaga gtgccagccg
    23701 ctgacgccag atcgttggct gaacgaaggc gataccatca gcatagggaa tgtgacttta
    23761 caggtgttac attgccctgg gcatacgccg ggtcatgtcg tgttttttga tgatcgggca
    23821 aagctgctga tttctggcga tgttattttc aaaggcggag tagggcgcag tgacttcccg
    23881 cgtggcgatc ataatcaact gatttcttca atcaaagata aattgctgcc actgggggat
    23941 gacgtgacat ttattccggg tcacggacca ttatccacac ttggttatga acgcctgcat
    24001 aatcccttcc tgcaagacga aatgcccgtc tggtaaggca cataaaaaag cccgctttta
    24061 atgcgggcct ggatttctgg caaagtgcgc tttgtttatg ccggatgcgg cacgagcgcc
    24121 ttatccggcc tacaaaatcg tgcaaattca aaatattgca ggggacgcgt aggcctgata
    24181 agcgtagcgc atcaggcaat gttgcgtttg tcatcagtct cagcccgctt ttcagcgggc
    24241 ttcattgttt ttaatgctta cagcactgcc acaatcgctt cgcacagcgg agccatgtta
    24301 tctggtgtca tcccggccac atttacgcga ccagaagcaa ccgcatatac gccaaactct
    24361 tcgcgcagac gcagcacttg ttcttttgtc aggccactga aggagaacat gccgttctgt
    24421 ttgatgataa agctgaagtc gcggtttgcg cctttttcct gcagcgtatt gacgaacaac
    24481 tgacgcatac gctgaatacg ctggcgcata tcagtcagct cttgttccca aatcgcacgt
    24541 aacgcatcgt tgctcaggat ggtggcaaca acagaagcgc cgtgtgctgg tgggttagag
    24601 tagttagcgc gaatcgccgc tttcatttgg ctgaatgcgc gatcaacggt ttcactgtcg
    24661 gcagcaacca gagtacaagc gccaacacgc tcgttgtaca ggccaaagtt tttagagtag
    24721 gaactggcaa caatcagctc tttatgcata gccgcgaaag cgcgcagtcc ttcagcatct
    24781 tcttccagac cacgggcaaa accctggtaa gcgaagtcaa acagcggtaa ccagcctttc
    24841 tcaactgaga gttgtgccag tgtctgccat tgttccagcg tagggtcgat accggttggg
    24901 ttatggcagc agccatggaa cagcactacg tcgccagcct gagcttcatt caggctgtta
    24961 atcagtgcat cgaagtcaag agtgtgattt tccgcatcat aataagcgta ttcacgaact
    25021 tccagacctg cagagttaaa gacgctctta tggttcggcc agcttgggtt gctcacccac
    25081 actcgcttaa cgctggtatt ttttgccagg aaatcggcag ctacgcgtag tgcgccagtg
    25141 ccccccggag tctgtgccgt gcgagcacgt ttgtcattga tcagggcgct acctttacca
    25201 aacagcagtt cctgagtgca gcgaccaaat tcagggatgc cgtcaatgcc gaggtaattt
    25261 ttggtggttt cattttcgag cagatactgt tcagcctttt tcacgctggt cagtaccggg
    25321 gttttgcccg tctcatcttt atagacacca atcccgaggt taattttgcc gggacgttca
    25381 tcggcacgaa acagatcggc caggcccaga atcgggtcgg caggagcggc ggtaatgttc
    25441 tcaaacatga cgaggttcca ttatggttac agaagggaag tccgctatca gggtaacggg
    25501 agatttacaa aattccaact attactgatg aaaacgcagg ctgtttttgc aagacgtgag
    25561 attgctctgg aaggtataaa aaaaacagga ccaaagtcct gttttttcgg catttaacaa
    25621 agaggtatgc tattagaact ggtaaacgat acccacagca acggtgtcgt ctgaacctac
    25681 gcccagtttg ttgtcagaat cgatctggtt gatgatgtag tcaacatagg tggacatgtt
    25741 tttgttgaag tagtaggttg cgcccacttc aaagtagttc accagatcaa catcaccgat
    25801 accttctacg tctttcgctt tagatttggt gtaagcgatg gacggacgca gaccgaaatc
    25861 gaactggtat tgcgcaacta acagaacgtc ttgcgttttg ttggcgaagc cgctggtgtt
    25921 tgtaaattta ttagtgatcg gcgtagcgtt acgggtttca ccgtagttcg ctgccaggta
    25981 gatgttgttc gcgtcgtact tcagaccagt agcccactgt tcagcttttt taccgttgcc
    26041 aagaggttga gcttcttgca ggttggtacg gtcagctgca ccataagcac caacgatacc
    26101 aaagccttcg tattcgtagc tgatagaacc gccaacaccg tcgccgttag aacggcgtgc
    26161 agtgtcacgc tcgtttttac ccaggtactg aacagcgaag ttcaggccat caaccagacc
    26221 aaagaagttg gagttacgat aggtagcaac gccgccaaca cgaccaacga agaagtcatc
    26281 gctgtatgca gtatcaccac caaattctgg cagcatatcg gtgtaaccca gtgcatcata
    26341 aaccacaccg tagttacggc cgtaatcgaa agaaccaacg tcagcgtatt taagacccgc
    26401 gaatgccaga cgcgttttgt taccagtttg agcgtcagcg ccttcagagt tgttaccctg
    26461 gaagttatat tcccactgac cataaccggt cagatcggaa ttgatttgag tttccccttt
    26521 aaaaccaaga cgggcatagg tcatgtcgcc attgccaccg taactgtttt caccgttacc
    26581 cttggaaaaa taatgcagac caacagcttt accgtacaga tctactttgt tgccatcttt
    26641 gttatagatt tctgcagcgt ttgcagtacc tgctactaac agagcaggga cgatcactgc
    26701 cagaatattg cgcttcatca ttatttatta ccctcatggt tttttttatg acacctgcca
    26761 ctgccgtcaa taagttctgt caataaaaat ttacggaact attgatgaga gtttggtgtc
    26821 tttatgtgtc tgcaggcatc tttccattca aactaacgtt tcgctaccg
//
LOCUS       DAPHDW010000055        26717 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.055, whole genome shotgun sequence.
ACCESSION   DAPHDW010000055 DAPHDW010000000
VERSION     DAPHDW010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.055"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..264
                     /gene="tuf"
                     /locus_tag="RH057_002273"
     CDS             <1..264
                     /gene="tuf"
                     /locus_tag="RH057_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3128633.1"
                     /translation="EVYILSKDEGGRHTPFFKGYRPQFYFRTTDVTGTIELPEGVEMV
                     MPGDNIKMVVTLIHPIAMDDGLRFAIREGGRTVGAGVVAKVLG"
     gene            447..641
                     /gene="bfd"
                     /locus_tag="RH057_002274"
     CDS             447..641
                     /gene="bfd"
                     /locus_tag="RH057_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HDV3128634.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            714..1190
                     /gene="bfr"
                     /locus_tag="RH057_002275"
     CDS             714..1190
                     /gene="bfr"
                     /locus_tag="RH057_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HDV3128635.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(1187..1654)
                     /locus_tag="RH057_002276"
     CDS             complement(1187..1654)
                     /locus_tag="RH057_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HDV3128636.1"
                     /translation="MDATLPFLILYACLSVLLFLWDAKHGLLPDRFTCPLLWSGLLFS
                     QVCNPDGLADALWGAIIGYGTFAVIYWGYRILRHKEGLGYGDVKFLAALGAWHTWTFL
                     PRLVFLAASFACGAVVVGLLMRGKESLKNPLPFGPFLAAAGFVVGWDSLLAGR"
     gene            2033..2344
                     /gene="rpsJ"
                     /locus_tag="RH057_002277"
     CDS             2033..2344
                     /gene="rpsJ"
                     /locus_tag="RH057_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HDV3128637.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2377..3006
                     /gene="rplC"
                     /locus_tag="RH057_002278"
     CDS             2377..3006
                     /gene="rplC"
                     /locus_tag="RH057_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HDV3128638.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            3017..3622
                     /gene="rplD"
                     /locus_tag="RH057_002279"
     CDS             3017..3622
                     /gene="rplD"
                     /locus_tag="RH057_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HDV3128639.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLHKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3619..3921
                     /gene="rplW"
                     /locus_tag="RH057_002280"
     CDS             3619..3921
                     /gene="rplW"
                     /locus_tag="RH057_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HDV3128640.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3939..4760
                     /gene="rplB"
                     /locus_tag="RH057_002281"
     CDS             3939..4760
                     /gene="rplB"
                     /locus_tag="RH057_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HDV3128641.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4777..5055
                     /gene="rpsS"
                     /locus_tag="RH057_002282"
     CDS             4777..5055
                     /gene="rpsS"
                     /locus_tag="RH057_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HDV3128642.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            5070..5402
                     /gene="rplV"
                     /locus_tag="RH057_002283"
     CDS             5070..5402
                     /gene="rplV"
                     /locus_tag="RH057_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HDV3128643.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VLVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5420..6121
                     /gene="rpsC"
                     /locus_tag="RH057_002284"
     CDS             5420..6121
                     /gene="rpsC"
                     /locus_tag="RH057_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HDV3128644.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            6134..6544
                     /gene="rplP"
                     /locus_tag="RH057_002285"
     CDS             6134..6544
                     /gene="rplP"
                     /locus_tag="RH057_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HDV3128645.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6544..6735
                     /gene="rpmC"
                     /locus_tag="RH057_002286"
     CDS             6544..6735
                     /gene="rpmC"
                     /locus_tag="RH057_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HDV3128646.1"
                     /translation="MKAKELREKSVEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6735..6989
                     /gene="rpsQ"
                     /locus_tag="RH057_002287"
     CDS             6735..6989
                     /gene="rpsQ"
                     /locus_tag="RH057_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HDV3128647.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            7154..7525
                     /gene="rplN"
                     /locus_tag="RH057_002288"
     CDS             7154..7525
                     /gene="rplN"
                     /locus_tag="RH057_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HDV3128648.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7536..7850
                     /gene="rplX"
                     /locus_tag="RH057_002289"
     CDS             7536..7850
                     /gene="rplX"
                     /locus_tag="RH057_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HDV3128649.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIVEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7865..8404
                     /gene="rplE"
                     /locus_tag="RH057_002290"
     CDS             7865..8404
                     /gene="rplE"
                     /locus_tag="RH057_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HDV3128650.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8419..8724
                     /gene="rpsN"
                     /locus_tag="RH057_002291"
     CDS             8419..8724
                     /gene="rpsN"
                     /locus_tag="RH057_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HDV3128651.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8758..9150
                     /gene="rpsH"
                     /locus_tag="RH057_002292"
     CDS             8758..9150
                     /gene="rpsH"
                     /locus_tag="RH057_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HDV3128652.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            9163..9696
                     /gene="rplF"
                     /locus_tag="RH057_002293"
     CDS             9163..9696
                     /gene="rplF"
                     /locus_tag="RH057_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HDV3128653.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9706..10059
                     /gene="rplR"
                     /locus_tag="RH057_002294"
     CDS             9706..10059
                     /gene="rplR"
                     /locus_tag="RH057_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HDV3128654.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            10074..10577
                     /gene="rpsE"
                     /locus_tag="RH057_002295"
     CDS             10074..10577
                     /gene="rpsE"
                     /locus_tag="RH057_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HDV3128655.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10581..10760
                     /gene="rpmD"
                     /locus_tag="RH057_002296"
     CDS             10581..10760
                     /gene="rpmD"
                     /locus_tag="RH057_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HDV3128656.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10764..11198
                     /gene="rplO"
                     /locus_tag="RH057_002297"
     CDS             10764..11198
                     /gene="rplO"
                     /locus_tag="RH057_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HDV3128657.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            11206..12537
                     /gene="secY"
                     /locus_tag="RH057_002298"
     CDS             11206..12537
                     /gene="secY"
                     /locus_tag="RH057_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HDV3128658.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12569..12685
                     /gene="rpmJ"
                     /locus_tag="RH057_002299"
     CDS             12569..12685
                     /gene="rpmJ"
                     /locus_tag="RH057_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HDV3128659.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12832..13188
                     /gene="rpsM"
                     /locus_tag="RH057_002300"
     CDS             12832..13188
                     /gene="rpsM"
                     /locus_tag="RH057_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HDV3128660.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            13205..13594
                     /gene="rpsK"
                     /locus_tag="RH057_002301"
     CDS             13205..13594
                     /gene="rpsK"
                     /locus_tag="RH057_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HDV3128661.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13628..14248
                     /gene="rpsD"
                     /locus_tag="RH057_002302"
     CDS             13628..14248
                     /gene="rpsD"
                     /locus_tag="RH057_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HDV3128662.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14274..15263
                     /gene="rpoA"
                     /locus_tag="RH057_002303"
     CDS             14274..15263
                     /gene="rpoA"
                     /locus_tag="RH057_002303"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HDV3128663.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15304..15687
                     /gene="rplQ"
                     /locus_tag="RH057_002304"
     CDS             15304..15687
                     /gene="rplQ"
                     /locus_tag="RH057_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HDV3128664.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTRILKCGF
                     RAGDNAPMAYIELVDRSEKAEAAAE"
     gene            15794..16162
                     /locus_tag="RH057_002305"
     CDS             15794..16162
                     /locus_tag="RH057_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1992 domain-containing protein"
                     /protein_id="HDV3128665.1"
                     /translation="MWLLDQWAERHIAEAQAKGEFDNLAGSGEPLILDDDSHVPPELR
                     AGYRLLKNAGCLPPELEQRREAIQLLDILKGIRHDDPQYQEVSRRLSLLELKLRQAGL
                     STDFLRGDYADKLLNKINDN"
     gene            16173..16598
                     /gene="zntR"
                     /locus_tag="RH057_002306"
     CDS             16173..16598
                     /gene="zntR"
                     /locus_tag="RH057_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(2+)-responsive transcriptional regulator"
                     /protein_id="HDV3128666.1"
                     /translation="MYRIGELAKMAEVTPDTIRYYEKQQMMEHEVRTEGGFRLYTESD
                     LQRLKFIRHARQLGFSLESIRELLSIRIDPEHHTCQESKGIVQERLQEVEARIAELQS
                     MQRSLQRLNDACCGTAHSSVYCSILEALEQGASGVKSGC"
     gene            16654..16872
                     /gene="arfA"
                     /locus_tag="RH057_002307"
     CDS             16654..16872
                     /gene="arfA"
                     /locus_tag="RH057_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome-rescue factor ArfA"
                     /protein_id="HDV3128667.1"
                     /translation="MSRYQHTKGQIKDNAIEALLHDPLFRQRVEKNKKGKGSYMRKGK
                     HGNRGNWEASGKKVNHFFTTGLLLSGVC"
     gene            complement(16869..17279)
                     /gene="mscL"
                     /locus_tag="RH057_002308"
     CDS             complement(16869..17279)
                     /gene="mscL"
                     /locus_tag="RH057_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HDV3128668.1"
                     /translation="MSIIKEFREFAMRGNVVDLAVGVIIGAAFGKIVSSLVADIIMPP
                     LGLLIGGIDFKQFAVTLRDAQGDIPAVVMHYGVFIQNVFDFLIVAFAIFMAIKLINKL
                     NRKKEEPAAAPAPTKEEVLLTEIRDLLKEQNNRS"
     gene            complement(17409..18785)
                     /gene="trkA"
                     /locus_tag="RH057_002309"
     CDS             complement(17409..18785)
                     /gene="trkA"
                     /locus_tag="RH057_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HDV3128669.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(18807..20096)
                     /gene="rsmB"
                     /locus_tag="RH057_002310"
     CDS             complement(18807..20096)
                     /gene="rsmB"
                     /locus_tag="RH057_002310"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HDV3128670.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRQQEELLAEFNASDARYLHPSWLLKRLQKAYPEQ
                     WQSIVEANNQRPPMWLRVNRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRVYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNLPGAEEGDGFFYAKLIKK"
     gene            complement(20142..21089)
                     /gene="fmt"
                     /locus_tag="RH057_002311"
     CDS             complement(20142..21089)
                     /gene="fmt"
                     /locus_tag="RH057_002311"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HDV3128671.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPVKVLAEEKGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINVHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(21104..21613)
                     /gene="def"
                     /locus_tag="RH057_002312"
     CDS             complement(21104..21613)
                     /gene="def"
                     /locus_tag="RH057_002312"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HDV3128672.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEADGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            21743..22867
                     /gene="dprA"
                     /locus_tag="RH057_002313"
     CDS             21743..22867
                     /gene="dprA"
                     /locus_tag="RH057_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HDV3128673.1"
                     /translation="MVDTEIWLRLMSISSLYGDDMVRIAHWLAKQSQIDAVGLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAIVGSRAHSWYGERWGRLFCETLATRGVTITSGLARGIDGVAHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLLEHGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            22839..23312
                     /locus_tag="RH057_002314"
     CDS             22839..23312
                     /locus_tag="RH057_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HDV3128674.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFDREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            23341..23883
                     /locus_tag="RH057_002315"
     CDS             23341..23883
                     /locus_tag="RH057_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase DNA-binding C4 zinc finger
                     domain-containing protein"
                     /protein_id="HDV3128675.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPACGENLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            23888..24460
                     /gene="tsaC"
                     /locus_tag="RH057_002316"
     CDS             23888..24460
                     /gene="tsaC"
                     /locus_tag="RH057_002316"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HDV3128676.1"
                     /translation="MNNNLQGDAIAAAIDVLNEERVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDAQRETIFSRWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            24465..25283
                     /gene="aroE"
                     /locus_tag="RH057_002317"
     CDS             24465..25283
                     /gene="aroE"
                     /locus_tag="RH057_002317"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HDV3128677.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GIGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            25280..25537
                     /locus_tag="RH057_002318"
     CDS             25280..25537
                     /locus_tag="RH057_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HDV3128678.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(25513..26067)
                     /locus_tag="RH057_002319"
     CDS             complement(25513..26067)
                     /locus_tag="RH057_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HDV3128679.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
     gene            26540..>26717
                     /locus_tag="RH057_002320"
     rRNA            26540..>26717
                     /locus_tag="RH057_002320"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         6877 a         6330 c         6929 g         6581 t
ORIGIN      
        1 gaagtgtaca ttctgtccaa agatgaaggc ggccgtcata ctccgttctt caaaggctac
       61 cgtccgcagt tctacttccg tactactgac gtgactggta ccatcgaact gccggaaggc
      121 gtagagatgg taatgccggg cgacaacatc aaaatggttg ttaccctgat ccacccgatc
      181 gcgatggacg acggtctgcg tttcgcaatc cgtgaaggcg gccgtaccgt tggcgcgggc
      241 gttgttgcta aagttctggg ctaattacac gttaattagt tttgaattga aaagggcgct
      301 tcggcgccct ttttgctgcc cgtaatttga gctcgaaata tttagtcgta attttgctcg
      361 ctgaactatg gcgcaaataa aactgtgcta ttgtaatcat aaccattctc atttacactt
      421 tgcgcagaat ttgaacggga gcaatcatgt acgtttgttt gtgtaatggt gtaagtgaca
      481 aaaaaattcg ccaggcagtc cgtcagtttc atccgcaatc tttccaacaa ttgcgtaagt
      541 ttattcctgt gggaaatcaa tgcggtaagt gcattcgcgc cgcgcgcgaa gtgatgcagg
      601 acgagttgat gcagatgcca gaatttaaag agattgcctg aagaccacgc ttattttttg
      661 acatcccgct agccccatct acgctctaat agtggaagcg gagggactat aaaatgaaag
      721 gtgatgttaa aatcataaat tatctcaata aactattggg aaatgagctt gtcgcaatca
      781 atcagtattt tctccatgcc cggatgttta aaaactgggg cctgatgcgt ctcaatgacg
      841 tcgaatacca tgaatccatc gatgagatga aacacgctga taaatatatc gagcgtattt
      901 tgtttttaga aggaatcccc aacttacagg atttaggcaa actggggatt ggtgaagatg
      961 tcgaggagat gctgcaatcc gacctgcgat tagaactgga aggcgcgaag gatctccgtg
     1021 aggctatcgc gtatgccgac agcgttcatg actatgttag ccgtgatatg atgatcgaga
     1081 tccttgccga tgaagaaggt catattgact ggctcgaaac tgagctggat ctcatcggta
     1141 aaatcggttt acaaaattac ctgcaatcac aaataaaagt taaagattaa cggcctgcca
     1201 gcaaactatc ccaacctaca acgaaacccg cagccgccag aaatggccca aacggcagcg
     1261 ggttttttaa tgactctttt cctctcatga gcagaccaac aactacggct ccgcaagcga
     1321 atgaggcagc aaggaaaacc agtcgtggca agaaggtcca ggtatgccag gcaccaaggg
     1381 cggcgagaaa cttcacatca ccatagccta atccttcttt atgacgcagt atgcgatacc
     1441 cccagtaaat gacagcgaat gtgccgtaac caataatcgc cccccataac gcatcggcta
     1501 aaccgtcagg attacaaacc tgagagaaca aaagacctga ccagagtaac gggcaggtaa
     1561 atctgtcagg taacaagcca tgctttgcat cccataaaaa aagtaggaca gataaacaag
     1621 cgtacaaaat cagaaaggga agggtagcgt ccataaaaca tcctctgtaa ttggagtatc
     1681 accatcgaat aactccggat gctcagcaac agagaaatcg ctgatttgtt aacctggttc
     1741 cggtgaaaaa cgctatcttc acaaccttac aaatttcgtg gaatacagct cgcaatctgc
     1801 gcgttgcgat taatttctac ccgatgcttg cgtcaggctc agatttgcgt ataatgcgcg
     1861 ggcttgtcgt agttgacagc aggttcaatc tgaacctcag ttagccgaat ttggctacct
     1921 aacaatgctc ccaatcgggg agctacgtaa gaacggttac actctcccat caatcgtaat
     1981 gggtctgagg agtaatcatt ttcgtttata aaataattgg agctctggtc tcatgcagaa
     2041 ccaaagaatc cgtatccgcc tgaaagcgtt tgatcatcgt ctgatcgatc aagcaaccgc
     2101 ggaaatcgtc gagactgcca agcgcactgg tgcgcaggtc cgtggtccga tcccgctgcc
     2161 gacacgcaaa gagcgcttca ctgttctgat ctccccgcac gtcaacaaag acgcgcgcga
     2221 tcagtacgaa atccgtactc acttgcgtct ggttgacatc gttgagccaa ccgagaaaac
     2281 cgttgatgct ctgatgcgtc tggatctggc tgccggtgta gacgtgcaga tcagcctggg
     2341 ttaatcaggt cattgagcga ttgagaggtt gaaacaatga ttggtttagt cggtaaaaaa
     2401 gtgggtatga cccgtatctt cacagaagac ggcgtttcta tcccagtaac cgtaatcgaa
     2461 gttgaagcaa accgcgttac tcaggttaaa gacctggcta acgatggcta ccgtgctatt
     2521 caggtgacca ccggtgctaa aaaagctaac cgtgtgacca agcctgaagc tggccacttc
     2581 gctaaagctg gcgtagaagc tggccgtggt ctgtgggaat tccgcctggc tgaaggcgaa
     2641 gagttcactg taggtcagag cattagcgtt gaactgtttg ctgacgttaa aaaagttgac
     2701 gtaactggca cctctaaagg taaaggtttc gcaggtaccg ttaagcgctg gaacttccgt
     2761 acccaggacg ctactcacgg taactccttg tctcaccgcg ttccgggttc tatcggtcag
     2821 aaccagactc cgggcaaagt gttcaaaggc aagaaaatgg caggtcagat gggtaacgaa
     2881 cgtgtaaccg ttcagagcct tgacgtagta cgcgttgacg ctgagcgcaa cctgctgctg
     2941 gttaaaggtg ctgtcccggg tgcaaccggt agcgacctga tcgttaaacc agctgtgaag
     3001 gcgtaaggag atagcaatgg aattagtatt gaaagacgcg cagagcgcgc tgactgtttc
     3061 cgaaactacc ttcggtcgtg atttcaacga agcgctggtt caccaggttg ttgttgctta
     3121 tgcagctggt gctcgtcagg gtactcgtgc tcagaagact cgtgctgaag taactggttc
     3181 cggtaaaaaa ccgtggcgcc agaaaggcac cggccgtgcg cgttctggtt ctatcaagag
     3241 cccgatctgg cgttctggtg gcgtgacctt tgctgctcgt ccgcaggacc acagtcaaaa
     3301 agttaacaag aagatgtacc gcggcgcgct gaaaagcatc ctgtccgaac tggtacgtca
     3361 ggatcgtctg atcgttgtcg agaagttctc tgtagaagcg ccgaaaacta agctgctggc
     3421 acagaaactg aaagacatgg ctctggaaga tgtgctgatc atcaccggtg agctggacga
     3481 aaacctgttc ctggctgcgc gcaacctgca caaggttgac gtacgcgatg caactggtat
     3541 cgacccggtt agcctgatcg ccttcgacaa agtcgtaatg actgctgatg ctgttaagca
     3601 agttgaggag atgctggcat gattcgtgaa gaacgtctgc tgaaggtgct gcgtgcaccg
     3661 cacgtttctg aaaaagcgtc tactgcgatg gaaaaatcca acaccatcgt actcaaagtt
     3721 gctaaagacg cgaccaaagc agaaatcaaa gctgctgtgc agaaactgtt tgaagtcgaa
     3781 gtcgaagtcg ttaacaccct ggtagttaaa gggaaagtta aacgtcacgg acagcgtatc
     3841 ggtcgtcgta gcgactggaa aaaagcttac gtcaccctga aagaaggcca gaatctggac
     3901 ttcgttggcg gcgctgagta agtcggagga gtaatacaat ggcagttgtt aaatgtaaac
     3961 cgacatctcc gggtcgtcgc cacgtagtta aagtggttaa ccctgagctg cacaagggca
     4021 aaccttttgc tccgttgctg gaaaaaaaca gcaaatccgg tggtcgtaac aacaatggcc
     4081 gtatcaccac tcgtcatatc ggtggtggcc acaagcaggc ttaccgtatt gttgacttca
     4141 aacgcaacaa agacggtatc ccggcagttg ttgaacgtct tgagtacgat ccgaaccgtt
     4201 ccgcgaacat cgcgctggtt ctgtacaaag acggtgaacg ccgttacatc ctggccccta
     4261 aaggcctgaa agctggcgac cagattcagt ctggcgttga tgctgcaatc aaaccaggta
     4321 acaccctgcc gatgcgcaac atcccggttg gttctactgt tcataacgta gaaatgaaac
     4381 caggtaaagg cggtcagctg gcacgttccg ctggtactta cgttcagatc gttgctcgtg
     4441 atggtgctta tgtcaccctg cgtctgcgtt ctggtgaaat gcgtaaagta gaagcagact
     4501 gccgtgcaac tctgggcgaa gttggcaatg ctgagcatat gctgcgcgtt ctgggtaaag
     4561 caggtgctgc acgctggcgt ggtgttcgtc cgaccgttcg cggtaccgcg atgaacccgg
     4621 tagaccaccc acatggtggt ggtgaaggtc gtaactttgg taagcacccg gtaactccgt
     4681 ggggcgttca gaccaaaggt aagaagaccc gcagcaacaa gcgtactgat aaattcatcg
     4741 tacgtcgccg tagcaaataa ttttagagga taagccatgc cacgttctct caagaaaggt
     4801 ccttttattg acctgcactt gctgaagaag gtagagaaag cggtggaaag cggagacaag
     4861 aagcccctgc gcacttggtc ccgtcgttca acgatctttc ctaacatgat cggtttgacc
     4921 atcgctgtcc ataatggtcg tcagcacgtt ccggtatttg taaccgacga aatggtcggt
     4981 cacaaactgg gtgaattcgc accgactcgt acttatcgcg gccacgctgc tgataaaaaa
     5041 gcgaagaaga aataaggtag gaggaagaga tggaaactat cgctaaacat cgccatgctc
     5101 gttcttctgc tcagaaggtt cgccttgttg ctgacctgat tcgcggtaag aaagtgtcgc
     5161 aggctctgga tattttgacc tacaccaaca agaaagcggc tgtactggtc aagaaagttc
     5221 tggaatctgc cattgctaac gctgaacaca acgatggcgc tgacattgac gatctgaaag
     5281 ttacgaaaat tttcgtagac gaaggcccga gcatgaagcg cattatgccg cgtgcaaaag
     5341 gtcgtgcaga tcgcatcctg aagcgcacca gccacatcac tgtggttgtg tccgatcgct
     5401 gagactctgg agactagcaa tgggtcagaa agtacatcct aatggtattc gcctgggtat
     5461 tgtaaaacca tggaactcta cctggtttgc gaacaccaaa gaattcgctg acaacctgga
     5521 cagcgatttt aaagtacgtc agtacctgac taaggaactg gctaaagcgt ccgtatctcg
     5581 tatcgttatc gagcgtccgg ctaagagcat ccgtgtaacc attcacactg ctcgcccggg
     5641 tatcgttatc ggtaaaaaag gtgaagacgt agaaaaactg cgtaaggtcg tagcggacat
     5701 cgctggcgtt cctgcacaga tcaacatcgc cgaagttcgt aagcctgaac tggacgcaaa
     5761 actggttgct gacagcatca cttctcagct ggaacgtcgc gttatgttcc gtcgtgctat
     5821 gaagcgtgct gtacagaacg caatgcgtct gggcgctaaa ggtattaaag ttgaagttag
     5881 cggccgtctg ggcggcgcgg aaatcgcacg taccgaatgg taccgcgaag gtcgcgtacc
     5941 gctgcacact ctgcgtgctg acatcgacta caacacctct gaagcgcaca ccacttacgg
     6001 tgtaatcggc gttaaagtgt ggatcttcaa aggcgagatc ctgggtggta tggctgctgt
     6061 tgaacaaccg gaaaaaccgg ctgctcagcc taaaaagcag cagcgtaaag gccgtaaata
     6121 aggagcgtcg ctgatgttac aaccaaagcg tacaaaattc cgtaaaatgc acaaaggccg
     6181 taaccgcggt ctggcgcagg gtacggatgt tagcttcggc agcttcggtc tgaaagctgt
     6241 tggccgtggt cgtctgactg cccgtcagat cgaagcagca cgtcgtgcta tgacccgtgc
     6301 agttaagcgt caaggtaaga tctggatccg tgtgttcccg gacaaaccga tcactgaaaa
     6361 gccgctggca gtgcgtatgg gtaaaggtaa aggtaacgtg gagtattggg ttgccttgat
     6421 tcagccgggt aaagtcctgt atgaaatgga cggtgttccg gaagagctgg cccgtgaagc
     6481 attcaagctg gcagcagcga aactgccgat taaaaccacc tttgtaacta agacggtgat
     6541 gtaatgaaag caaaagagct gcgtgagaag agcgttgaag agctgaacac cgagctgctg
     6601 aacctgctgc gtgagcagtt caacctgcgt atgcaggctg caagtggcca gctgcaacag
     6661 tctcacctgt tgaagcaagt gcgtcgcgat gtcgcacgcg ttaagacttt actgaacgag
     6721 aaggcgggtg cgtaatgacc gataaaatcc gtactctgca aggtcgcgtt gttagcgaca
     6781 aaatggagaa atccattgtt gttgctatcg aacgttttgt gaaacacccg atctacggta
     6841 aattcatcaa gcgtacgacc aaactgcacg tacatgacga gaacaacgaa tgcggtatcg
     6901 gtgacgtggt tgaaatccgc gaatgccgtc cgctgtccaa gactaaatcc tggacgctgg
     6961 ttcgcgttgt agagaaagcg gttctgtaat acagtacact ctctcgatac gaataaacgg
     7021 ctcagaaatg agccgtttat tttttctacc catatccttg aagcggtgtt ataatgccgc
     7081 gccctcgata tggggatttt taacgacctg attttcgggt ctcagtagta gttgacatta
     7141 gcggagcact aaaatgatcc aagaacagac tatgctgaac gtcgccgaca actccggtgc
     7201 acgtcgcgta atgtgtatca aggttctggg tggctcgcac cgtcgctacg caggcgtagg
     7261 cgacatcatc aagatcacca tcaaagaagc aattccgcgt ggtaaggtca aaaaaggtga
     7321 tgtgctgaag gcggtagtgg tgcgcaccaa gaagggtgtt cgtcgcccgg acggttctgt
     7381 cattcgcttc gatggtaatg cttgtgttct tctgaacaac aacagcgagc agcctatcgg
     7441 tacgcgtatt tttgggccgg taactcgtga gcttcgtagt gagaagttca tgaaaattat
     7501 ctctctggca ccagaagtac tctaaggagc gaatcatggc agcgaaaatc cgtcgtgatg
     7561 acgaagttat cgtgttaacc ggtaaagata aaggtaaacg cggtaaagtt aagaatgtcc
     7621 tgtcttccgg caaggtcatt gttgaaggta tcaacctggt taagaaacat cagaagccgg
     7681 ttccggccct gaaccaaccg ggtggcatcg ttgaaaaaga agccgctatt caggtttcca
     7741 acgtagcaat cttcaatgcg gcaaccggca aggctgaccg tgtaggcttt agattcgaag
     7801 acggcaaaaa agtccgtttc ttcaagtcta acagcgaaac tatcaagtaa tttggagtag
     7861 tacgatggcg aaactgcatg attactacaa agacgaagta gttaaaaaac tcatgactga
     7921 gtttaactac aattctgtca tgcaagtccc tcgggtcgag aagatcaccc tgaacatggg
     7981 tgttggtgaa gcgatcgctg acaaaaaact gctggataac gcagcagcag acctggcagc
     8041 aatctccggt caaaaaccgc tgatcaccaa agcacgcaaa tctgttgcag gcttcaaaat
     8101 ccgtcagggc tatccgatcg gctgtaaagt aactctgcgt ggcgaacgca tgtgggagtt
     8161 ctttgagcgc ctgatcacta ttgctgtacc tcgtatccgt gacttccgtg gcctgtccgc
     8221 taagtctttc gacggtcgtg gtaactacag catgggtgtc cgtgagcaga tcatcttccc
     8281 agaaatcgac tacgataaag tcgaccgcgt tcgtggtttg gatattacca ttaccactac
     8341 tgcgaaatct gacgaagaag gccgcgctct gctggctgcc tttgacttcc cgttccgcaa
     8401 gtaaggtagg gttactaaat ggctaagcaa tcaatgaaag cacgcgaagt aaaacgcgta
     8461 gctttagctg ataaatactt cgcgaaacgc gctgaactga aagcgatcat ctctgatgtg
     8521 aacgcttccg acgaagatcg ttggaacgct gttctcaagc tgcagactct gccgcgtgat
     8581 tccagcccgt ctcgtcagcg taaccgctgc cgtcaaacag gtcgtccgca tggtttcctg
     8641 cggaagttcg ggttgagccg tattaaggtc cgtgaagccg ctatgcgcgg tgaaatcccg
     8701 ggtctgaaaa aggctagctg gtaattgtca ccaattgaat cacgggagta aagacagatg
     8761 agcatgcaag atccgatcgc ggatatgctg acccgtatcc gtaacggtca ggccgcgaac
     8821 aaagctgcgg tcaccatgcc ttcctccaag ctgaaagtgg caatcgccaa cgtgctgaag
     8881 gaagaaggtt ttattgaaga ttttaaagtt gaaggcgaca ccaagcctga actggaactt
     8941 actctgaagt atttccaggg caaagctgtt gtagaaagca ttcagcgtgt cagccgccca
     9001 ggtctgcgca tctataaacg taaagatgag ctgccgaaag ttatggcggg tctgggtatc
     9061 gcagttgttt ctacctctaa aggtgttatg actgatcgtg cagcgcgcca ggctggtctt
     9121 ggtggcgaaa ttatctgcta cgtagcctaa tcggaggaaa aaatgtctcg tgttgctaaa
     9181 gcaccggtcg ttgttcctgc cggcgttgac gtaaaaatca acggtcaggt tattacgatc
     9241 aaaggtaaaa acggcgagct gactcgtact ctcaacgatg ctgttgaagt taaacatgca
     9301 gataataccc tgaccttcgg tccgcgtgat ggttacgcag acggttgggc acaggctggt
     9361 accgcgcgtg ccctgctgaa ctcaatggtt atcggtgtta ccgaaggctt cactaagaag
     9421 ctgcagctgg ttggtgtagg ttaccgtgca gcggttaaag gcaatgtgat taacctgtct
     9481 ctgggtttct ctcatcctgt tgaccatcag ctgcctgcgg gtatcactgc tgaatgtccg
     9541 actcagactg aaatcgtgct gaaaggcgct gataagcagg tgatcggcca ggttgcagcg
     9601 gatctgcgcg cctaccgtcg tcctgagcct tataaaggca agggtgttcg ttacgccgac
     9661 gaagtcgtgc gtaccaaaga ggctaagaag aagtaaggta acactatgga taagaaatct
     9721 gctcgtatcc gtcgtgcgac ccgcgcacgc cgcaagctcc aggagctggg cgcaactcgc
     9781 ctggtggtac atcgtacccc gcgtcacatt tacgcacagg taattgcacc gaacggttct
     9841 gaagttctgg tagctgcttc tactgtagaa aaagctatcg ctgaacaact gaagtacacc
     9901 ggtaacaaag acgcggctgc agctgtgggt aaagctgtcg ctgaacgcgc tctggaaaaa
     9961 ggcatcaaag atgtatcctt tgaccgttcc gggttccaat atcatggtcg tgtccaggca
    10021 ctggcagatg ctgcccgtga agctggcctt cagttctaag gtagaggtgt aagatggctc
    10081 acatcgaaaa acaagctggc gaactgcagg aaaagctgat cgcggtaaac cgcgtatcta
    10141 aaaccgttaa aggtggtcgt attttctcct tcacagctct gactgtagtt ggcgatggta
    10201 acggtcgcgt tggttttggt tacggtaaag cgcgtgaagt tccagcagcg atccagaaag
    10261 cgatggaaaa agcccgtcgc aatatgatta acgtcgcgct gaataacggc actctgcaac
    10321 accctgttaa aggtgttcac acgggttctc gcgtattcat gcagccggct tccgaaggta
    10381 ccggtatcat cgccggtggt gcaatgcgcg ccgttctgga agtcgctggg gttcataacg
    10441 ttctggctaa agcatatggt tccaccaacc cgatcaacgt ggttcgtgca actattgatg
    10501 gcctggaaaa tatgaattct ccagaaatgg tcgctgccaa gcgtggtaaa tccgttgaag
    10561 aaattctggg gaaataaacc atggcaaaga ctattaaaat tactcaaacc cgcagtgcaa
    10621 tcggtcgtct gccgaaacac aaggcaacgc tgcttggcct gggtctgcgt cgtattggtc
    10681 acaccgtaga gcgcgaggat actcctgcta ttcgcggtat gatcaacgcg gtttccttca
    10741 tggttaaagt tgaggagtaa gagatgcgtt taaatactct gtctccggcc gaaggctcca
    10801 aaaaggcggg taaacgcctg ggtcgtggta tcggttctgg cctcggtaaa accggtggtc
    10861 gtggtcacaa aggtcagaaa tctcgttctg gcggtggcgt acgtcgcggt ttcgagggtg
    10921 gtcagatgcc tctgtaccgt cgtctgccga aattcggctt cacttctcgt aaagcagcga
    10981 ttacagccga agttcgtctg tctgacctgg ctaaagtaga aggcggtgtt gtagacctga
    11041 acacgctgaa agcggctaac attatcggta tccagatcga gttcgcgaaa gtgatcctgg
    11101 ctggcgaagt aacgactccg gtaactgttc gtggcctgcg tgttactaaa ggcgctcgtg
    11161 ctgctatcga agctgctggc ggtaaaatcg aggaataagt agcagatggc taaacaaccg
    11221 ggattagatt ttcaaagtgc caaaggtggc ttaggcgagc tgaaacgcag actgctgttt
    11281 gttatcggtg cgctgattgt gttccgtatt ggctctttta ttccgatccc tggtattgat
    11341 gccgctgtac ttgccaaact gcttgagcaa cagcgaggca ccatcattga gatgtttaac
    11401 atgttctctg gtggtgctct cagccgtgct tctatctttg ctctggggat catgccgtat
    11461 atttcggcgt cgatcattat ccagctgctg acggtggttc acccaacgtt ggcagaaatt
    11521 aagaaagaag gggagtctgg tcgtcgtaag atcagccagt acacccgtta cggtactctg
    11581 gtgctggcaa tattccagtc gatcggtatt gctaccggtc tgccgaatat gcctggtatg
    11641 caaggcctgg tgattaaccc aggctttgca ttctacttca ctgctgttgt aagtctggtc
    11701 acaggaacga tgttcctgat gtggttgggc gaacagatta ctgaacgagg tatcggcaac
    11761 ggtatttcaa tcattatctt cgccggtatt gtcgcgggac tcccgccagc cattgcccat
    11821 actatcgagc aagcgcgtca aggcgacctg cacttcctcg tgttgctgtt ggttgcagta
    11881 ttagtatttg cagtgacgtt ctttgttgta tttgttgagc gtggtcaacg ccgcattgtg
    11941 gtaaactacg cgaaacgtca gcaaggtcgt cgtgtctatg ctgcacagag cacacattta
    12001 ccgctgaaag tgaatatggc gggggtaatc ccggcaatct tcgcttccag tattattctg
    12061 ttcccggcga ccatcgcgtc atggttcggg ggcggtactg gttggaactg gctgacaaca
    12121 atttcgctgt atttgcagcc tgggcaaccg ctttatgtgt tactctatgc gtctgcaatc
    12181 atcttcttct gtttcttcta cacggcgttg gttttcaacc cgcgtgaaac agcagataac
    12241 ctgaagaagt ccggtgcatt tgtaccagga attcgtccgg gagagcaaac ggcgaagtat
    12301 atcgataaag taatgacccg cctgaccctg gttggtgcgc tgtatattac ctttatctgc
    12361 ctgatcccgg agttcatgcg tgatgcaatg aaagtaccgt tctacttcgg tgggacctca
    12421 ctgcttatcg ttgttgtcgt gattatggac tttatggctc aagtgcaaac tctgatgatg
    12481 tccagtcagt atgagtctgc attgaagaag gcgaacctga aaggctacgg ccgataattg
    12541 gtcgcccgag aagttacgga gagtaaaaat gaaagttcgt gcttccgtca agaaattatg
    12601 ccgtaactgc aaaatcgtta agcgtgatgg tgtcatccgt gtgatttgca gtgccgagcc
    12661 gaagcataaa cagcgccaag gctgattttt tcgcatattt ttcttgcaaa gttgggttga
    12721 gctggctaga ttagccagcc aatcttttgt atgtctgtgc gtttccattt gagtatcctg
    12781 aaaacgggct tttcagcatg gaacgtacat attaaatagt aggagtgcat agtggcccgt
    12841 atagcaggca ttaacattcc tgatcataag catgccgtaa tcgcattaac ttcgatttat
    12901 ggcgtcggca agacccgttc taaagccatc ctggctgcag cgggtatcgc tgaagatgtt
    12961 aagatcagtg agctgtctga aggacaaatc gacacgctgc gtgacgaagt tgccaaattt
    13021 gtcgttgaag gtgatctgcg ccgtgaaatc agcatgagca tcaagcgcct gatggatctt
    13081 ggttgctatc gcggtttgcg tcatcgtcgt ggtctcccgg ttcgcggtca gcgtaccaag
    13141 accaacgcac gtacccgtaa gggtccgcgc aaaccgatca agaaataatc ggggtgattg
    13201 aataatggca aaggcaccaa ttcgtgcacg taaacgtgta agaaaacaag tctctgacgg
    13261 cgtggctcat atccatgctt ctttcaacaa caccatcgtg actatcactg atcgtcaggg
    13321 taacgcgttg ggttgggcaa cagccggtgg ttccggtttc cgtggttctc gcaaatccac
    13381 tccgtttgca gctcaggttg cagcagagcg ttgcgctgac gccgtgaaag aatacggcat
    13441 caagaatctg gaagttatgg ttaaaggtcc gggtccaggc cgcgaatcta ctattcgtgc
    13501 tctgaacgcc gcaggtttcc gcatcactaa tattactgat gtgactccga tccctcataa
    13561 cggttgtcgt ccgccgaaaa aacgtcgcgt ataacgcctc gttttccagg tttgttggag
    13621 aaagaaaatg gcaagatatt tgggtcctaa gctcaagctg agccgtcgtg agggcaccga
    13681 cttattcctt aagtctggcg ttcgcgcgat cgataccaag tgtaaaattg aacaagctcc
    13741 tggccagcac ggtgcgcgta aaccgcgtct gtctgactat ggtgtgcagt tgcgtgaaaa
    13801 gcaaaaagtt cgccgtatct atggtgtgct ggagcgtcag ttccgtaact actacaaaga
    13861 agcagcacgt ctgaaaggca acaccggtga aaacctgttg gctctgctgg aaggtcgtct
    13921 ggacaacgtt gtataccgta tgggcttcgg tgccactcgt gcagaagcac gtcagctggt
    13981 tagccataaa gcaattatgg taaacggtcg tgttgttaac atcgcttctt atcaggttag
    14041 tccgaatgac gttgtaagca ttcgtgagaa agcgaagaag cagtctcgcg tgaaagccgc
    14101 tctggagctg gctgagcagc gtgaaaagcc aacctggctg gaagttgatg ctggcaagat
    14161 ggaaggtacg tttaagcgta agccggagcg ttctgatctg tctgcggaca ttaacgaaca
    14221 cctgatcgtc gagctttact ccaagtaaag cttagtacca aagagaggac acaatgcagg
    14281 gttctgtgac agagtttcta aaaccgcgcc tggttgatat cgagcaagtg agttcgacgc
    14341 acgccaaggt gacccttgag cctttagagc gtggctttgg ccatactctg ggtaacgcac
    14401 tgcgccgtat tctgctctca tcgatgccgg gttgcgcggt gaccgaggtt gagattgatg
    14461 gtgtactaca tgagtacagc accaaagaag gcgttcagga agatatcctg gaaatcctgc
    14521 tcaacctgaa agggctggcg gtgagagttc agggcaaaga tgaagttatt cttaccttga
    14581 ataaatctgg cattggccct gtgactgcag ccgatatcac ccacgacggt gatgtcgaaa
    14641 tcgtcaagcc gcagcacgtg atctgccacc tgaccgatga gaacgcgtct attagcatgc
    14701 gtatcaaagt tcagcgcggt cgtggttatg tgccggcttc tacccgaatt cattcggaag
    14761 aagatgagcg cccaatcggc cgtctgctgg tcgacgcatg ctacagccct gtggagcgta
    14821 ttgcctacaa tgttgaagca gcgcgtgtag aacagcgtac cgacctggac aagctggtca
    14881 tcgaaatgga aaccaacggc acaatcgatc ctgaagaggc gattcgtcgt gcggcaacca
    14941 ttctggctga acaactggaa gctttcgttg acttacgtga tgtacgtcag cctgaagtga
    15001 aagaagagaa accagagttc gatccgatcc tgctgcgccc tgttgacgat ctggaattga
    15061 ctgtccgctc tgctaactgc cttaaagcag aagctatcca ctatatcggt gatctggtac
    15121 aacgtaccga ggttgagctc cttaaaacgc ctaaccttgg taaaaaatct cttactgaga
    15181 ttaaagacgt gctggcttcc cgtggactgt ctctgggcat gcgcctggaa aactggccac
    15241 cggcaagcat cgctgacgag taaccggatc acaggttaag gttttactga gaaggataag
    15301 gtcatgcgcc atcgtaagag tggtcgtcaa ctgaaccgca acagcagcca tcgccaggct
    15361 atgttccgca atatggcagg ttcactggtt cgtcatgaaa tcatcaagac gactctgcct
    15421 aaagcgaaag agctgcgccg cgtagttgag ccgctgatta ctcttgccaa gactgatagc
    15481 gttgctaatc gtcgtctggc attcgcccgt actcgtgata acgagatcgt ggcaaaactg
    15541 tttaacgaac tgggcccgcg tttcgcgagc cgtgccggtg gttacactcg tattctgaag
    15601 tgtggcttcc gtgcaggcga caacgcgccg atggcttaca tcgagctggt tgatcgttca
    15661 gagaaagcag aagctgctgc agagtaatct gaagcaacgt aaaaaaaccc gccccggcgg
    15721 gtttttttat acccgtagca tccccactta tctacaatag ctgtactctt tttgttcatc
    15781 ccctggagta tatatgtggt tacttgacca gtgggcagag cgtcatatag cagaagcgca
    15841 agcgaaaggt gagtttgata acctggcagg tagcggcgaa ccattgatac tggatgatga
    15901 ttctcacgtg ccaccggaat tacgtgcggg gtatcgcttg ttgaagaatg ccggttgctt
    15961 accgccagaa cttgagcaac ggagggaagc aattcagctt ctggatattc tcaaaggtat
    16021 ccgtcacgat gatccgcaat atcaagaggt tagccgtcga ttgtcattac tggaattgaa
    16081 gctgcgacaa gctggactga gtaccgattt tttacgcggc gattatgctg acaagttgtt
    16141 gaataaaatc aacgataact agtggagtat gtatgtatcg cattggtgag ctggcaaaaa
    16201 tggcggaagt aacacccgac acgattcgtt attacgaaaa acagcagatg atggagcatg
    16261 aagtgcgtac tgaaggtggg tttcgcctgt ataccgaaag cgatctccag cgattgaaat
    16321 ttatccgcca tgccagacaa ctaggtttca gtctggagtc gatccgcgag ttgctgtcga
    16381 tccgtatcga tcctgaacac catacctgtc aggagtcaaa aggcattgtg caggaaagat
    16441 tgcaggaagt cgaagcacgg atagccgagt tgcagagtat gcagcgttcc ttgcaacgcc
    16501 ttaacgatgc ctgttgtggg accgcccata gcagtgttta ttgctcgatt cttgaagctc
    16561 ttgaacaagg ggcgagtggc gttaagagtg gctgttgatt ttttgcactg acaggattac
    16621 actcgcgccg ttaaataacc aactggagtt tttatgagcc gatatcagca tactaaaggg
    16681 cagataaagg ataatgcgat agaagcatta ctacatgatc ccttattccg acagcgagta
    16741 gagaaaaata agaaagggaa aggcagttac atgcgaaaag gaaaacatgg caatcggggg
    16801 aactgggagg ccagtggcaa gaaagtaaat cactttttta ccactggcct tctgctttca
    16861 ggcgtttgtt aagagcggtt attctgctct ttcagcaaat cacgaatttc tgtcagtaat
    16921 acttcttctt tagttggtgc tggtgcggct gctggttctt cttttttccg attcagtttg
    16981 ttgattagct taatcgccat aaagatggca aaggccacaa tcagaaaatc aaagacgttt
    17041 tgaatgaaga caccgtaatg catcacaaca gcagggatat ccccctgcgc atcgcgtagc
    17101 gtgacagcaa actgtttaaa gtcgatcccg ccaattaata agcccagagg tggcatgatg
    17161 atatcggcaa ccagtgaaga tacaatcttc ccgaatgccg caccgataat gacacccacc
    17221 gccaaatcca ccacgttccc gcgcatcgca aattcgcgaa attctttaat aatgctcatg
    17281 ttattctccc tatgaagccg acaaccataa gtctaacaaa tgttaagcca ttttcctgcc
    17341 aggaatgaat ttaattaatc cttgataatc aaagggaaat aataaggcgt cattagacgc
    17401 cttattaatt acaagaagaa agggcttggc tggaagagtc tttcgacgtc ggtaataaac
    17461 tttttatctg tgaggaacat aattacgtga tcgccttgct caatgcgcag attgtcattg
    17521 gcaatcatca cgtcgtttcc acgtaccacc gctccaataa tcgttcctgg cggtagcttg
    17581 atttcgtcaa tgactctgcc gacaacgcgt gaggtgcttt catcaccgtg agcaacggct
    17641 tcaatagctt ctgctacgcc gcggcgcaat gaggaaacac caacaatatc tgcttttcgc
    17701 acatggctaa gcaacgcaga aatagttgct tgttgtggtg aaatcgcaat atcgatcacg
    17761 ctcccctgaa ccagatccac ataagcgcga cgctggatca ataccatcac ctttttcgca
    17821 cccatacgtt tggcaagcat ggcggacatg atattggcct cgtcatcgtt ggtgacggca
    17881 ataaacagat caacttgatc gatatgttct tcggccagca gttcttgatc cgacgcatca
    17941 ccaaaaaaga cgatcgtatt ctgcaacttt tccgccagtt cggcagcgcg ctgctgatta
    18001 cgttcgatga gtttaacgct gtaatctttt tccagacgac gcgccagccc tgcaccgata
    18061 ttaccgccac caaccagcat gatccgctta tacggttttt ccagtcgctg taattcactc
    18121 atcaccgcac ggatatgctg tgaagcggca ataaagaaca cttcatcacc agcttccaca
    18181 atggtcgaac cttgtggacg aatggggcga tcgtggcgga aaattgctgc cacacgagta
    18241 tcgatatgtg gcatatgttc gcgcatggtc gaaagtgcgt taccaatcag cgggccgcca
    18301 taataggctt taaccacagc gaggctgact ttaccctcag cgaagttcac cacctgcaat
    18361 gcgccgggat actcaatcag tcggtaaata ttatcgataa ccaactgctc tggtgcgatc
    18421 agatgatcaa tcggtacagc atctgaatga aatagcttat cggcatcgcg cacgtagtct
    18481 ggtgagcgga tgcgagcgat gcgattaggg gtgttgaaaa gtgagtaggc tacctggcag
    18541 gcaaccatat tggtttcatc tgaactggtt acagcaacca gcatatcggc gtcgtcggca
    18601 cctgcctccc gcaatacgcg tggatgagag ccatgcccct gcacgactcg caggtcaaat
    18661 ttatcctgca aggtccgcag acgctcaccg ttggtatcga caacagtgat atcgttgttc
    18721 tcgccaacca ggttttccgc cagtgtgccg ccaacctggc cggcacccag aatgataatt
    18781 ttcatcagtc gcgacccgtt atctcatcac tttttgatta gcttagcgta aaagaagccg
    18841 tcgccctctt cggcaccagg cagattttgt ttacccggtt gctctggtgt tcctgtttcg
    18901 caaagttcgg catcagcggt acgttgcaaa aaggctttaa tctgcaggct attctcttcc
    18961 ggtaacaccg aacaggtggc atagaccagg gttccaccgg attttaaatg cggccaaatg
    19021 gcgtcgagaa tttcagactg caattgcgcg agttccggga tatcgcgatc gcgacgtaac
    19081 catttaatat ccggatggcg acgaatcaca ccggttgctg aacaaggcgc atctaataaa
    19141 atgcgatcaa actgttgctc gccacaccat tgggaagggt agcggccatc accttgtttc
    19201 acggtcgctt tcataccaag gcgttttaaa ttgtcgtaaa cgcgagagag gcgctgttcg
    19261 tcaatatcaa ccgcaacaac ctgcgcttct ggtgccacct caaggatatg cgttgtttta
    19321 ccgccggggg cggcacaaag atccagaatg tgttcaccgt tttgtggcgc aagccaggtc
    19381 atgcaacctt gtgctgatgc atcctgaacg gtaacccatc cgtcttcaaa accaggtagc
    19441 gcatgaacag gtgcaggtgt ttccagccgt acagcatcag ggtaatccgc atgcgggaaa
    19501 cctttcattc ctgcttcatc caacaatgca agccagctgt cgcgggaatg atgcgtacga
    19561 ttaacgcgca gccacattgg cggacgctgg ttattggctt cgacgatgga ttgccactgc
    19621 tctggatacg ctttttgcag acgcttcagc aaccaggaag gatgcagata acgtgcatca
    19681 ctggcattaa actcggctaa taactcttct tgctgacgct ggaactggcg taatacaccg
    19741 tttatcaacc ctttaagttg cggacgctta attgcgatag cgccttcaac cgtttcagcc
    19801 agcgcagcat gaggtggaat gcgggtataa agcagttgat acaaaccaac cataatcagg
    19861 taatgcacgg tccgctgttt gccggtcatc ggacgggcca ttaacttatt aatcagccag
    19921 tctaactgcg aaagcgtacg cagtacgcca aagcacaact cttgaagaag tgctttgtct
    19981 ttatcggaaa ctttttgctg gagcggtggc agaatgttgc ttaatgattg cccttgctcg
    20041 acgacttgtt caacggcctg ggccgccatg ctacgtaaat tacgttgttt tttcataagt
    20101 ataaaaacgc ccggcaagac cgggcttaga agagtggact atcagaccag acggttgccc
    20161 ggaacaaacc attcccgacg agagttcagg aggtcttgcg cgctcatcgc tttcttaccc
    20221 gcaggttgta acgagagcag gttcaggatg ccatcaccag tcgcaacctg aatgccttgt
    20281 ttgttggctt caaggatcgt tcctggtgca gcgttggttg ccgtatcaat gaccgatgct
    20341 ttccagactt taaccggctg tccttcaatt tccagccagc tcattggcca tggattgaaa
    20401 gcgcgaatgc agcgttcaag ctgtgctgcc gaaagtgacc agtcaatacg cgcttcttct
    20461 ttactcaact tttcggcgta agtgacaaga gtttcgtcct gaacttctgg tttcgccgtg
    20521 ccgtctgcca gttgtttcaa cgtggtgata agcccttgtg ggccaagctc tgccagcttg
    20581 tcgtacagcg taccactggt atcttctgca gtaatcgggc aggagagctt atagagcatg
    20641 tcaccggtgt ctaaaccgac atccatttgc ataatggtca caccagtttc tgcatcaccc
    20701 gcccatagtg agcgttggat tggtgcagca ccgcgccagc gtggcagcag tgaaccatga
    20761 acgttgatac agccaagacg cggcatctcc agcactgctt taggcagaat taagccatag
    20821 gcgacgacga ccataacatc agcctgcaga tcggcgacca gttgctggtt ttcttgtgga
    20881 cgcagggaaa caggttgaaa aacgggcaga cctttttcct cagccagaac tttaaccggg
    20941 ctgggcatca gttttttacc gcgtcctgcc ggtcggtctg gctgggtgaa cacgccaacg
    21001 acgttatgac cagaagacaa cagcgcgtcg agatgacgcg ctgcaaagtc aggtgtaccc
    21061 gcaaaaataa tacgtagtga ttctgacacg ttagttctta tccttaagcc cgggctttca
    21121 gacgatccag tttttcaact ttctgacgaa tacgttgttg tttcaacggt gacagataat
    21181 ccataaacag tttgccgacc aggtgatcca tctcatgctg aatacagatg gctaacagac
    21241 cgtctgcttc caattcaaat ggtttaccgt cgcggtccag ggcgcgaatt ttaactttct
    21301 ctgcgcgtgg cactaaagca cgttgttcag ggatcgacag gcaaccttct tcaatgcctg
    21361 tttcgccgct tttttctaaa agctccggat tgattaacac cagccgttcg tcacggtttt
    21421 ccgaaacatc aataacaatg atacgttgat ggatatcaac ctgggttgcc gccaggccaa
    21481 taccttcttc tgcgtacatc gtctcgaaca tatcatcgac gatacgctga atttctgcat
    21541 tcacttcttc taccggttta gcaactttgc gaagccgctc gtccggaata tgtaacactt
    21601 gcaaaactga cataaatctc cagagatgtg ttcaggagtt agaaagatta tttcttctat
    21661 tctagacaaa tccccctctg attgacagca tcactgacca atcgcaaaga ttgctaaggc
    21721 tgcttatggc agggagataa ggatggtcga tacagaaatt tggctgcgtt taatgagtat
    21781 cagcagcttg tacggcgatg atatggtccg tatagctcac tggctggcaa aacagtcgca
    21841 aattgatgcg gttggattgc agcaaacagg gcttacattg cggcaggcac aacgctttct
    21901 ttcatttccg cgaaagagta tcgaaagctc actttgttgg ttggagcaac ccaaccatca
    21961 tttaatccct gcggacagcg aattttatcc tcctcaactt caggcgacga cagattaccc
    22021 cggcgcactg tttgttgaag gagaactgca cgcgctgcat tcatttcagc ttgccatagt
    22081 ggggagtcgg gcgcattcat ggtatggcga gcgatgggga cgattatttt gcgaaactct
    22141 ggcgacgcgt ggagtgacaa ttacaagtgg actggcgcgt ggaatcgatg gtgtggcgca
    22201 taaagcggcc ttacaggtaa atggcgtcag cattgctgta ttggggaatg gacttaatac
    22261 cattcatccc cgccgccatg cccgactggc tgccagtttg cttgaacatg gcggagctct
    22321 cgtctcggaa tttcccctcg atgttccacc ccttgcttac aatttcccac gaagaaatcg
    22381 cattatcagt ggtctaagta aaggtgtact ggtggtggaa gcggctttgc gcagtggttc
    22441 gctagtgaca gcacgttgtg cgcttgagca ggggcgtgaa gtttttgcct tgccaggtcc
    22501 aatagggaat ccgggaagcg aagggcctca ctggttaata aaacaaggtg cgattcttgt
    22561 gacggaaccg gaagaaattc tggaaaactt gcaatttgga ttgcactggt tgccagacgc
    22621 ccctgaaaat tcattttatt caccagatca gcaagacgtg gcattgccat ttcctgagct
    22681 cctggctaac gtaggagatg aggtaacacc tgttgacgtc gtcgctgaac gtgccggcca
    22741 acctgtgcca gaggtagtta ctcaactact cgaactggag ttagcaggat ggatcgcagc
    22801 tgtacccggc ggctatgtcc gattgaggag ggcatgccat gttcgacgta ctaatgtatt
    22861 tgtttgaaac ctatattcac acagaagctg agttgcgtgt ggatcaagac aaacttgaac
    22921 aggatcttac cgacgcagga tttgatcgag aagatattta caatgccctg ctttggctgg
    22981 aaaagcttgc ggattatcag gaaggactgg cagaaccgat gcaactggcc tctgatccac
    23041 tctccatgcg tatttatacc cctgaggagt gtgaaagact ggatgccagc tgtcgtggtt
    23101 ttctgctttt ccttgagcag attcaggtgc tcaaccttga aactcgtgaa atggtgatag
    23161 agcgagtgct cgcgctggat aacgcagagt tcgaactgga tgatctgaaa tgggtaatcc
    23221 tgatggtgtt gttcaatatt ccgggctgcg aaaatgcgta ccagcaaatg gaagaattac
    23281 tctttgaagt gaatgaaggt atgctgcatt aatcatttct ttaattcagc ataagttgtt
    23341 atggcgaaat cagcactgtt cacggtgcgt aataatgagt cctgcccaaa gtgcggggct
    23401 gaactggtta ttcgatccgg gaaacacggt ccgtttcttg gatgctcaca gtatccggcg
    23461 tgtgactacg tccgtcctct gaaatcttca gcggatggac atattgtcaa agttctggag
    23521 gggcaggttt gccctgcatg tggcgaaaat ctggtattac gccagggacg ctttggtatg
    23581 tttattggtt gcagtaacta ccctgaatgc gaacataccg aacttatcga taaaccggac
    23641 gaaacagcaa ttacatgccc ccaatgccgg acgggccatc tggtccagcg ccgctcccgt
    23701 tatggcaaaa catttcactc ctgtgatcgc tacccggagt gtcaatttgc cattaacttc
    23761 aaacccatag ctggagaatg ccctgagtgt cattatccac tactcatcga aaagaaaacc
    23821 gcgcagggtg taaaacactt ttgtgccagt aaacaatgtg gaaagccggt ttcggcggaa
    23881 taataacgtg aataataacc tgcaaggaga cgctatcgca gctgcgatag atgtcctcaa
    23941 tgaagaacgt gtcatcgcct atccaacgga agccgttttc ggtgtcgggt gcgatcctga
    24001 tagcgaaaca gcagtgatgc gactgttgga gttaaaacag cgtccggttg ataaggggct
    24061 gattttaatc gcggcaaatt acgagcagct taaaccctat attgatgaca ccatgctgac
    24121 tgatgcgcag cgtgaaacca ttttttcccg ctggccaggt cctgtcacct ttgtctttcc
    24181 cgcgcctgcg acaacaccgc gctggttgac gggccgcttc gattcgcttg ctgtacgagt
    24241 caccgaccat ccgttggtgg ttgctttgtg ccaggcttat ggtaaaccgc tggtttctac
    24301 cagtgccaac ttgagtggat tgccaccttg tcgaacagta gacgaagttc gcgcacaatt
    24361 tggcgcggcg ttcccggttg tgcctggtga aacggggggg cgtttaaatc cttcagaaat
    24421 ccgcgatgcc ctgacgggtg aactgtttcg acaggggtaa cataatggaa acctatgctg
    24481 tttttggtaa tccgatagcc cacagcaaat cgccattcat tcatcagcaa tttgctcagc
    24541 aactgaatat tgaacatccc tatgggcgcg tgttggcacc catcaatgat ttcatcaaca
    24601 cactgaacgc tttctttcgt gctggtggta aaggtgcgaa tgtgacggtg ccttttaaag
    24661 aagaggcttt tgccagagcg gatgagctta ctgaacgggc agcgttggct ggtgctgtta
    24721 ataccctcaa gcggttagaa gatggacgcc tgctgggtga caataccgat ggtataggtt
    24781 tgttaagcga tctggaacgt ctgtctttta tccgtcctgg tttacgtatt ctgctcatcg
    24841 gcgctggtgg agcatctcgc ggcgtactac tgccacttct ttccctggac tgtgcggtga
    24901 caatcactaa tcggacggta tcccgcgcgg aagagttggc taaattgttt gcgcacactg
    24961 gcagtattca ggcgttgggt atggacgaac tggaaggtca tgagtttgat ctcattatta
    25021 atgcaacatc cagtggcatc agtggtgata ttccggcgat cccgtcatcg ctcattcatc
    25081 caggcattta ttgctatgac atgttctatc agaaaggaaa aactcctttt ctggcatggt
    25141 gtgagcagcg aggctcaaag cgtaatgctg atggtttagg aatgctggtg gcacaggcgg
    25201 ctcatgcctt tcttctctgg cacggtgttc tgcctgacgt agaaccagtt ataaagcaat
    25261 tgcaggagga attgtccgcg tgaatcaggc catccagttt ccggacagag aagagtggga
    25321 cgagaataaa aaatgtgtat gttttcccgc tctcgtgaat ggcatgcaac tgacatgtgc
    25381 gatctctggc gagagtctgg cgtatcgttt tactggagat acgccagaac agtggttagc
    25441 gagttttcgt cagcatcgct gggatctgga agaagaagcg gaaaacttaa ttcaggaaca
    25501 aagtgaagat gatcaaggct gggtctggtt accctgatcc agatattcgt ccttccattt
    25561 cacgtaatta ttcgcggaat agcgtaaccc agccttctct tcatcactta acgggcggat
    25621 ctgtttgacg gggctaccga gatacagata tccgctctcc agccgtttat tttgtgggac
    25681 cagactaccc gcaccaatca tcacatcatc ttctactatt gcgccatcaa gtaaaattga
    25741 gcccatccca accaaaactc gattgccaat ggtgcagccg tggagcatca ccttgtgacc
    25801 aacagtgaca tcttcgccaa tggttaatgg gttgccatct gggttgtacg aggatttatg
    25861 agtgacatgc aacatactgc catcctggat attggtgcgt gctccgatct gtacataatg
    25921 tacatctcca cgaatcacaa cgagcggcca gatccccaca tcatcagcca gacgaacgtc
    25981 accaatcacg acactgctat cgtcgatcat tacgcgctga ccgatttgtg gaaaaagatc
    26041 gcggtatggg cgtaaaacat cagacatact tacctcagca ataaatgatt tactaatgac
    26101 tttgggggca ttattggcct tgtgcaagtc ttttagtatg caaaaaagca ccgttttgtg
    26161 tgcgaatgca gcaaaaaggg tgaaaaaaca acaaacagaa aaaaagatca aaaaagtact
    26221 tgtgcaaaaa attgggatcc ctataatgcg cctccgttga gacgacaacg tgaaacactt
    26281 cacaagatgg tcgtaacaac gaagagaaaa aatcctgaaa ttcagggttg actctgaaag
    26341 aggaaagcgt aatatacgcc acctcgcgac agtgagctga aagctgcgtc gcaactgctc
    26401 tttaacaatt tatcagacaa tctgtgtggg cactcgaaga tacggattct taacgtcgca
    26461 agacgaaaaa tgaataccaa gtctcaagag tgaacacgta attcattacg aagtttaatt
    26521 ctttgagcat caaactttta aattgaagag tttgatcatg gctcagattg aacgctggcg
    26581 gcaggcctaa cacatgcaag tcgaacggta acaggaaaca gcttgctgtt tcgctgacga
    26641 gtggcggacg ggtgagtaat gtctgggaaa ctgcctgatg gagggggata actactggaa
    26701 acggtagcta ataccgc
//
LOCUS       DAPHDW010000056        26438 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.056, whole genome shotgun sequence.
ACCESSION   DAPHDW010000056 DAPHDW010000000
VERSION     DAPHDW010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26438
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.056"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            184..900
                     /gene="nanC"
                     /locus_tag="RH057_002321"
     CDS             184..900
                     /gene="nanC"
                     /locus_tag="RH057_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HDV3128680.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            920..2026
                     /gene="nanM"
                     /locus_tag="RH057_002322"
     CDS             920..2026
                     /gene="nanM"
                     /locus_tag="RH057_002322"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HDV3128681.1"
                     /translation="MNKTITALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPRDQATSAFIDGNLYVFGGIGKNSKGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSATIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAALVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLAPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            2091..3071
                     /locus_tag="RH057_002323"
     CDS             2091..3071
                     /locus_tag="RH057_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HDV3128682.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHPRIKQL
                     ARFAHTHPGGPSCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPSIPDNAGVLIVPCCRGGSAFIAGSEGTYSERHGASHDACRWGSDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGVISDRFVEAILQFWREK"
     gene            complement(3079..3201)
                     /locus_tag="RH057_002324"
     CDS             complement(3079..3201)
                     /locus_tag="RH057_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012139841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV3128683.1"
                     /translation="MISFVFLLPVYPNCHAMLHRRKPPFTPEELKALMDANKSN"
     gene            3283..4155
                     /locus_tag="RH057_002325"
     CDS             3283..4155
                     /locus_tag="RH057_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004186812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV3128684.1"
                     /translation="MASSKSLQQAIANIKIWHKGEQRAPHKPLLLLYVLAGYLNGHPR
                     LFDYGSEIYEPLHSLLERFGPQRSHYRPDMPFWRLQGDGFWQLHNAELCSTAGSSRQP
                     PVKELNEYHVAGGFDEQHYALVTGNKKLINTLAQQILEAHFTESIQEEIADELGFDLQ
                     QIRKQRDPLFRKNVLRAYNYQCAICGFNMRHDDTTVALEAAHIKWKQQGGPCEIPNGL
                     ALCAIHHKAFDKGSIGLDENMRVLVSDAVNGGGIVERLFWDFDGKTIALPQVRKNYPY
                     EVFVEWHRKEVFRG"
     gene            4325..6400
                     /locus_tag="RH057_002326"
     CDS             4325..6400
                     /locus_tag="RH057_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000177022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV3128685.1"
                     /translation="MTLVDSVEAGRLTISELIDALAKDKNYTASRWYQRYRAFTTLLQ
                     QTSTFAEPATDGLVKQLWYERDNGIASIRQGVPSLAEYQQSLPLLRELTERIRQQPDE
                     ETYQYVGNALQQAKENGLLKRMYWSLRNRVFAAFSPENYTSTVDENAFNKAAEFLNQH
                     FHLGLVLTGNWLQKNYELKQAIHAQSPDTDPYYVNMAIWHLYELLRERDNEQKQEKVA
                     STTSITRSEPIENKIILHSPTNVIFFGPPGTGKTFRLQQKMKEYTSHAVPADRDAWLD
                     SRLESLNWMQVITLVLLDLGKRAKVRQIIEHMWFQRKALLNGRNGNLSNTAWAALQSY
                     TVPESLTVDYKNRREPAVFNKTDNSEWFLVDSQLEQVEDLVELYAELKRGPKSAEAIQ
                     RFAVVTFHQSYGYEEFIEGIRARSDESGNISYPIEPGIFMRLCQRANADPGHRYAIFI
                     DEINRGNISKIFGELISLIEVDKRAGMPNAMSLQLAYSGDHFSVPGNVDIIGAMNTAD
                     RSLALMDTALRRRFDFVEMMPDLSLLSEAKVKGIELESLLEKLNSRIEALYDREHTLG
                     HAFFMPVKNALDAGDEEAAFKQLKIAFQKKIIPLLQEYFFDDWNKIRLVLADNQKQDD
                     NLQFVIEKTDDLDTLFGNNHGLRRHDQQSTAYELKDFDQEIWNIPQAYRSIYQPQQTP
                     LDEQAVNHG"
     gene            6393..7736
                     /locus_tag="RH057_002327"
     CDS             6393..7736
                     /locus_tag="RH057_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HDV3128686.1"
                     /translation="MGEVISVFEYDLLGSGKAASVGAKPVPPQVFNYLEALSLASNQG
                     SQFLKLTSRSGFKLLQVQNYAGMLSTPHGFQLEILPKVGKNLTAANARQTLLTMLSHL
                     PGFRHIQTQQATLQAQRMPLLEIFINQFLHSVSQLLKQGLRSDYVSKQGNLAFMKGKL
                     MLSAQLRHNAVNRHKFCVDYDDYMPDCAANRLLHSALDKLLSLQLSSDNQRWLYELRF
                     AFDGIPLSRDIERDINNLRLERGMAHYNEPMAWAQLILRGMSPSALQGNTKAISLLFP
                     MEAVFESFVAQTLPDELPPHLKVLPQAATYSLVKHGLKDCFKLRPDLLIQSHKPVQTE
                     MVMDTKWKLVNSSQQTKSLYGLAQADFYQMFAYGQKYLGGNGEMYLIYPAHDDFSQPI
                     AQHFAFSETLKLWVVPYRIMAKRGERMMWPNDVSCTMSPKLDRMAASVSYSGGMR"
     gene            7733..8119
                     /locus_tag="RH057_002328"
     CDS             7733..8119
                     /locus_tag="RH057_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004222267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128687.1"
                     /translation="MTHEYQRQSLEQLITQQTATPRSAAVVFNENQTLWQTLGFSQSQ
                     VSLWLASLPQSNHLKGPTTATYQVIPDIAAHLVSLLHQAGGRMPLAQVLKKLPAGITT
                     SEQQIRKLAQKHAQIEIKGPLLVLIN"
     gene            8230..9369
                     /locus_tag="RH057_002329"
     CDS             8230..9369
                     /locus_tag="RH057_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128688.1"
                     /translation="MVKQHSEFTQPLVELPGSAGRLLKQVRDIEQVAPLFRDAGLVKA
                     VPKPTIVNLRVAGIATKSCLDKVLGGFIYASAAVRTDLLIDDNKVSTVGSDSVSELDD
                     IDFEAQRKRLDLIEIRQAYQLVEKKLLGYEPGDLILLDTPLFLARDMAPLERNVRHKQ
                     EYEKTRQVIETFWQNHRSRIFPWNQNGVVLASILAERFSAIVSIAKQDLRTEQGRKQI
                     LASDGFSKELMQNLEGLDESLVGVGDLRFINGILGNYSRTIAFRLSEQEKRIEPNIEA
                     KQGLIGFHFRSARGGQIKMVQLAGDEPEWKSADLDLVASRLMVLDMQNKGNAMPLPQL
                     LGYQQLGILPKFATFYRQGLHQALKNNDIEAGWLAGLDEGLNNGL"
     gene            9359..11044
                     /locus_tag="RH057_002330"
     CDS             9359..11044
                     /locus_tag="RH057_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019106735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HDV3128689.1"
                     /translation="MAYKVLGKLVGNTGDASQLTMVVQDSFSVRRGEFVRIMHQERKD
                     EGQVAVLGRVTKISRTNMLYNAGFGDGVTELELLPGANVTGENMFAQVELVGYRDPVT
                     RQIKIPRRPLNPGTAVETVDFQFLSDFYEFNEHSSLHLGNLVGYDKGENTVPVFIDVN
                     KLVTEHLAVLAMTGSGKSYTVGRIIERLVAVNNGTVVVFDPHGEYGKALAGGNLQFSS
                     FLEATDDKRDQEALPLIKQTFEKLQAAGAGIQVYSPQHESFKHKYASKNTPLALQFDH
                     FEMDDIAEILPGLTEPQQRVLDVAIRYWRSADKTEPRDINRLRFLLGDGIEELKEWDD
                     LSEAEAKALSGRSAAVASMKLSRVLNEAKSFYSATMAEPTDIYKMVGRPSNQQGRLVV
                     VDLQGLSDTAKQVICALLSSEILKAASSKTDPLRPCFIIYEEGHNFAPAGGNAVSHRI
                     IKKIAGEGRKFGVGFGIVSQRPSKLDSDVTSQCNTLITMRLKNPDDQRFIAKASDMVS
                     KADLEELPSLSTGEALVCGRSIPAPLLVKVGSKALIHGGESPEVLRVWGSFSG"
     gene            11181..11594
                     /locus_tag="RH057_002331"
     CDS             11181..11594
                     /locus_tag="RH057_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128690.1"
                     /translation="MAVVECPAPGTFGADIRSDSGWFHKSSASPVCLIEFERFDGSAK
                     GQQKLEEKLKNLLEAAQRWNHCPKTLVLSAWSQGLVGVPDTQKLKDICRMGFTSSTGT
                     QVIAAPDVEVVFSRFLFIKNLNMIVLDRIHYEVLM"
     gene            11591..13060
                     /locus_tag="RH057_002332"
     CDS             11591..13060
                     /locus_tag="RH057_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDV3128691.1"
                     /translation="MNIKSAHFLPGTNKRLGDKYLLLECLGDGSHGWVWRAERLKDGK
                     IVAVKIPKDITREDRQLAEGKELLNVEPHENIVQIFDMGRIDNEWFYIEMEYFPSQTL
                     AQKLDDRQRNFGQTYERLFRIYRQVLCAVHYLAELPVPISHGDIKPHNILVGERDLVK
                     LTDFGSSALPEEIYVRTRENGGTVLYSAPEFSNVDSRRGSLEELLLGDIYSLGVLLYQ
                     LLTGKLPHDTPAQVQRHAPFKLPTEINSSIHTDLEQVVLTCLQKRAEDRFSTISDLIH
                     AFDSAATKQLKVGAIAPVFQTKPAQDWSSAVLEAMSDQSYQKAAQLASQEYSRSKDLQ
                     ALHQQLIALYRANRLFDFEKVVEDNKAILLEGKLTQPAALFELIVKANLQLRNISQAN
                     FWLLQRKQAEAENAATMYLESSILGLEAKYSEARVLLEKVNQTTPMKFHVLSQLILVC
                     EQMRDYSGAAAYLKAALRVAPLDNSMKEKKELYAKLGVI"
     gene            complement(13262..14526)
                     /locus_tag="RH057_002333"
                     /pseudo
     CDS             complement(13262..14526)
                     /locus_tag="RH057_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003924703.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(14728..14812)
                     /locus_tag="RH057_002334"
     tRNA            complement(14728..14812)
                     /locus_tag="RH057_002334"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14776..14778),aa:Leu,seq:caa)
     gene            15008..16027
                     /gene="ahr"
                     /locus_tag="RH057_002335"
     CDS             15008..16027
                     /gene="ahr"
                     /locus_tag="RH057_002335"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HDV3128692.1"
                     /translation="MSMIKCYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALAGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIKHVRDGKARYRVV
                     LKADF"
     gene            16157..17659
                     /locus_tag="RH057_002336"
     CDS             16157..17659
                     /locus_tag="RH057_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HDV3128693.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGVAQAGTASEKLLARLKNIGVNDWQPHANPVVVWDIFG
                     EKGHPVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDTNGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVLGQLGNRVQHALRAFTPKDQKAV
                     KAAAQTMRANPAFDTEKAIQELGTGEALISFLDVKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGVQASAEQQNNPPAKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRRR"
     gene            complement(17778..18860)
                     /gene="lptG"
                     /locus_tag="RH057_002337"
     CDS             complement(17778..18860)
                     /gene="lptG"
                     /locus_tag="RH057_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313266.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV3128694.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEELGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTNLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(18860..19960)
                     /gene="lptF"
                     /locus_tag="RH057_002338"
     CDS             complement(18860..19960)
                     /gene="lptF"
                     /locus_tag="RH057_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV3128695.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            20227..21738
                     /gene="pepA"
                     /locus_tag="RH057_002339"
     CDS             20227..21738
                     /gene="pepA"
                     /locus_tag="RH057_002339"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HDV3128696.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGLAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMANHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            21872..22315
                     /gene="holC"
                     /locus_tag="RH057_002340"
     CDS             21872..22315
                     /gene="holC"
                     /locus_tag="RH057_002340"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HDV3128697.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGGAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            22315..25170
                     /gene="valS"
                     /locus_tag="RH057_002341"
     CDS             22315..25170
                     /gene="valS"
                     /locus_tag="RH057_002341"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HDV3128698.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EDEVRKENNLGADVVLRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(25225..26421)
                     /locus_tag="RH057_002342"
     CDS             complement(25225..26421)
                     /locus_tag="RH057_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HDV3128699.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFCYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSMKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTTYCIKYAILAFLALLPFLAVAGYIIIDQILNAYDSSVYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLARISRGIM
                     PSLPFL"
BASE COUNT         7191 a         6249 c         6569 g         6429 t
ORIGIN      
        1 ctgtttattt aaataatgtg aaaagagatt tttcacagga gaccttatac aaaaaaatat
       61 aaaatacagc taccggttgc caaagacact ataagcctgg caaaaaaata ttacacaaca
      121 taaatgctaa ttgtttatgc gggctttgta ttgctttctg tatcctacaa atgagtgaaa
      181 tttatgaaaa aggctaaaat actttctggc gtattattac tgtgcttttc gtccccatta
      241 atttctcagg ctgcgacact ggacgtacgt ggtggatatc gtagtggaag ccacgcctat
      301 gagactcgac tcaaagtcag tgagggatgg caaaatggat ggtgggcaag catggaaagt
      361 aatacctgga ataccattca tgataataaa aaggaaaatg ccgcactcaa tgatgttcag
      421 gttgaagtta attacgcgat taaacttgat gatcaatgga cggtgcgccc gggaatgtta
      481 acgcatttta gcagcaacgg cacacgctac ggaccctacg taaaactgtc ctgggacgcg
      541 acaaaagatc ttaattttgg cattcgctat cgttacgact ggaaagctta ccgacaacaa
      601 gacttatccg gtgatatgtc tcgtgataac gttcatcgtt gggatggata tgtcacttac
      661 catattaata gtgatttcac cttcgcatgg caaacgacgc tatacagcaa acagaacgat
      721 tatcgctatg caaaccataa gaaatgggcg acggaaaatg catttgttct acaataccat
      781 atgacgcccg atattacgcc atacatagaa tatgactacc tggaccgtca gggtgtttac
      841 aacggcagag ataatttatc ggaaaacagt tatcgcattg gtgtgtcatt taaactgtag
      901 tagacaggag acagtcacaa tgaataaaac aataacggcg cttgctatca tgatggcttc
      961 atttgccgca aacgcgtctg tattacctga aactcctgtg ccatttaaaa gtggtaccgg
     1021 agcaattgat aacgacactg tctacattgg tttaggtagc gcaggtacgg catggtacaa
     1081 gctggataca caggccaaag ataaaaaatg gacagcgtta gctgcattcc ctggtggacc
     1141 aagagatcaa gcaacctcgg catttattga tggcaatctg tatgtgtttg gcggcattgg
     1201 caaaaatagc aagggcttga ctcaggtatt taatgacgta cacaaataca accccaaaac
     1261 caatagttgg gttaaattga tgtcgcatgc accgatgggc atggcgggtc atgtgacttt
     1321 tgtacacaac ggcaaggctt atgttactgg cggtgttaac cagaatatct tcaatggcta
     1381 ttttgaagat ctcaacgagg ctggaaaaga ttcagcaact atagacaaga tcaatgccca
     1441 ttattttgac aaaaaagcag aagattattt cttcaataag tttctgttgt cttttgatcc
     1501 ctcaacacag caatggagtt acgctggcga atctccctgg tacggaacgg ctggtgcggc
     1561 gcttgtgaat aaaggtgata aaacctggct tattaatggc gaagccaaac caggattgcg
     1621 aacggatgcc gtatttgaac ttgatttcac cggtaataat ttaaaatgga ataagcttgc
     1681 tcccgtctca tcaccagatg gcgtcgctgg cggttttgcg gggataagca atgattctct
     1741 tatatttgcc ggaggggccg gattcaaagg ttcacgagaa aattaccaga acggtaagaa
     1801 ctatgcgcat gaaggcctaa aaaaatcata tagcactgat attcatcttt ggcataacgg
     1861 gaaatgggat aaatcgggtg aattatcgca aggtcgggcc tacggagtat cattgccctg
     1921 gaataatagt ctattgatta ttggcggtga aactgcaggc ggcaaagcgg tgacggattc
     1981 agttttgatc tctgtgaagg ataataaagt cacagtacaa aattaacgct tcagggcccc
     2041 ggtaaagggg ccctgctatc aatttgtcat ttcaattacg cgaaatttat atgaacgcaa
     2101 taatatcgcc cgattattac tatgttctta ccgttgctgg tcagtctaat gccatggcgt
     2161 atggcgaagg actgccatta ccggacaggg aagatgcgcc tcatcccaga attaaacaat
     2221 tagcgagatt tgcgcatacg catcccggag gcccgtcatg tcactttaac gacattattc
     2281 cactgactca ctgcccacac gatgttcagg atatgcaggg ttatcaccat cctctggcaa
     2341 cgaatcatca aacacagtac ggcaccgttg gccaggcact gcatattgca cggaaattac
     2401 tgccctctat tcctgataat gcaggggttc tcatcgttcc atgttgtcgt ggcggctcgg
     2461 cttttatcgc gggcagcgaa gggacatatt cagaacggca cggagccagc catgatgctt
     2521 gtcgttgggg atcggatact ccgctatacc aggatttagt cagcagaacg cgagccgcac
     2581 tggcaaaaaa tccgcagaac aaattcctcg gcgtatgctg gatgcaaggc gaatttgact
     2641 taatgaccag tgactacgcg tcacaccctc aacactttaa tcatatggtt gaagcctttc
     2701 gtagggatct aaaacaatac cattctcagc ttaataatat tactgacgcg ccgtggtttt
     2761 gcggcgatac cacctggtac tggaaagaaa atttcccgca ttcgtatgaa gctatttatg
     2821 gcaattatca aaataatgtt ttagccaata ttattttcgt cgacttccag caacaaggtg
     2881 aaagaggact gacgaacgcg cctgatgaag acccggacga tttaagcacg ggatattacg
     2941 gttcagcgta ccggtcaccg gagaactgga cgacggcact gcgaagcagt catttcagca
     3001 cggcagcccg tcggggggtt atttctgaca ggtttgtaga agcaattttg cagttttggc
     3061 gcgaaaagtg agcgttcatt aatttgattt attcgcatcc atcaacgctt ttaactcctc
     3121 cggcgtaaaa ggtggtttgc gtcggtgcaa catcgcatgg caattagggt acacaggcag
     3181 caaaaaaaca aatgagatca aagtaatata tcttcacttg caggcttgaa cagccgaaaa
     3241 tttgcgagca tgatcgcaat tatttcactg ccagggatat caatggcttc cagtaaatcg
     3301 ctacagcagg caattgccaa cataaaaatc tggcacaaag gtgaacagcg cgcgccgcat
     3361 aagccattgt tattgctata cgtattagcg ggatacctga atggacatcc acgccttttc
     3421 gattatggat cggaaatcta cgaacctctg cacagtttac tggaacgttt tgggccacag
     3481 cgttcacatt accgacctga tatgccgttc tggcgattac aaggcgacgg attctggcaa
     3541 ttacacaatg ccgagctttg ctcaacagca gggagcagcc gacaaccgcc agtgaaggaa
     3601 ctgaacgaat accacgttgc aggcggtttc gatgaacagc actacgccct ggtaaccggc
     3661 aataaaaaac tcatcaatac tctggcgcaa cagatactcg aagcgcactt caccgagagt
     3721 attcaggaag aaattgctga cgagctgggt tttgatctcc agcaaatccg taaacagcgc
     3781 gaccctttat ttcgcaaaaa tgtattacgc gcttataact accagtgcgc gatatgtggc
     3841 tttaatatgc gccacgacga caccaccgta gcgttggaag cggcgcatat aaaatggaaa
     3901 cagcaaggag gtccttgtga aattcctaat ggcctggcgc tttgcgcaat tcaccataaa
     3961 gcctttgata aaggatcgat tggcctggat gaaaatatgc gggtactggt atcagatgcg
     4021 gtaaacggcg gagggatcgt tgagagatta ttctgggatt ttgacgggaa aactattgcg
     4081 ttgccgcagg tgcgtaaaaa ttacccttat gaggtgtttg tagagtggca tcggaaagag
     4141 gtttttaggg ggtgaggttg ataacagcat tcaccctaca accttacatt gtatttaaat
     4201 tagagctgag ttaacagata gttgtatata taacaggcac ttgtcacaac gattaactca
     4261 atatagttag aatacctcat attaattaaa aaataagtaa tgtcacaaca gaggaaaagt
     4321 cacgatgact cttgtagata gtgtcgaagc aggtaggcta acgattagtg aattgattga
     4381 tgccctggcg aaagataaaa attacacagc ttccagatgg tatcagcgat accgtgcatt
     4441 tacgactttg ctacagcaaa cctcaacttt tgctgagcct gcaacagatg gtctggtcaa
     4501 acagctttgg tatgagcgtg acaacggcat tgcaagtatt cgccagggcg ttccatcctt
     4561 agcagaatat cagcaaagcc tgccactgct tagagaacta actgaacgaa ttcggcaaca
     4621 gccggatgaa gaaacttacc aatatgttgg caatgcactt caacaagcta aagaaaacgg
     4681 acttctcaag cgtatgtatt ggagtttgag aaatcgcgtc tttgccgcgt tctcgccaga
     4741 aaactacacc agtactgtgg atgagaatgc ttttaataaa gcagcagaat tcttaaatca
     4801 gcacttccat ctcggtttgg tactgaccgg aaattggtta cagaaaaact atgaattgaa
     4861 acaagccata cacgcccaat ctcctgatac agatccttat tatgtgaata tggccatctg
     4921 gcatctctat gaattgctcc gtgaacgcga taatgaacaa aagcaggaga aagtagctag
     4981 cactacatcc ataacccgca gtgagcccat cgagaacaag atcatcctac attcaccaac
     5041 taacgtgatc ttctttggcc cccctggcac tggcaagacc ttcaggttgc agcaaaaaat
     5101 gaaagagtac acttctcatg ctgttcccgc tgatcgtgat gcctggctgg attctcgcct
     5161 tgaatcgttg aactggatgc aggttataac gctggtgctg ctcgatcttg ggaaacgagc
     5221 gaaagttcgc caaattattg aacatatgtg gtttcaacgt aaggcattat taaacggtcg
     5281 taatggcaat ctatcgaata ctgcctgggc agctttgcaa tcctatacag ttcccgagtc
     5341 gttaaccgtt gattataaga atcggcgtga gcctgccgta tttaacaaaa cagataacag
     5401 cgaatggttt ctagttgatt cacagctcga gcaagtggag gatttggtag agctctacgc
     5461 cgaacttaaa cgtggcccta aatctgccga agccatccag cgttttgcgg tggttacgtt
     5521 ccaccaatct tacggctatg aagaatttat tgaaggtata cgcgctcgct ctgacgagag
     5581 tggcaatatc tcttatccca ttgagccggg tatctttatg cgcctttgcc aacgtgcgaa
     5641 tgccgatcca ggacatcgct acgccatttt cattgatgag atcaatcgcg gcaacatatc
     5701 caagatcttt ggtgaactaa tctcactcat tgaagtagac aagcgtgcag gcatgcccaa
     5761 tgcgatgagc ctgcaactgg cttatagcgg tgatcacttc agcgtacccg gcaatgtcga
     5821 tatcatcgga gccatgaata cagcggaccg ttctttagct ctgatggaca cggctttgcg
     5881 ccgtcgcttt gactttgtcg aaatgatgcc tgatctctct ttactgagtg aagctaaggt
     5941 gaaaggcata gagctcgagt cgttgttaga gaaactcaat agccgcatcg aggctcttta
     6001 cgatcgtgaa catacgctgg ggcatgcgtt ctttatgccg gtaaaaaatg cactcgatgc
     6061 cggtgatgaa gaagctgcgt ttaaacaatt gaagatcgca ttccagaaaa agatcattcc
     6121 gcttttacag gaatactttt tcgatgactg gaacaagatc cggttggtgc tggcagacaa
     6181 tcaaaagcaa gacgacaacc tgcaattcgt gattgagaaa accgacgatc tcgatacgct
     6241 ttttggtaac aaccatggtt tacgacgcca tgatcagcaa tcaacagctt atgagctcaa
     6301 agatttcgat caagagatct ggaatattcc acaggcttat cgttcaattt atcagcccca
     6361 acagactccc cttgatgagc aggcagtaaa tcatgggtga agttatctcc gtttttgaat
     6421 atgacctctt ggggagtgga aaagcggctt cagttggcgc aaaaccggtg cctccacagg
     6481 tctttaatta tttagaagcg cttagcctgg caagtaatca agggagccag ttcctcaaac
     6541 tcacctctcg ctcaggcttc aaattgcttc aggttcaaaa ctacgcaggg atgctttcaa
     6601 cacctcacgg tttccagctt gagatactgc ccaaggttgg taaaaacctc acagcggcta
     6661 atgcacgtca aacattgctg acgatgctaa gccatctgcc tgggtttcga catattcaaa
     6721 cccagcaagc cactcttcag gctcagcgta tgcctttgct tgagattttt attaaccagt
     6781 ttttgcatag cgtcagccaa ttgcttaaac agggtttacg ctccgactat gtgagcaagc
     6841 aaggtaatct ggcttttatg aagggcaagc tgatgctttc tgcacagctg cgacataacg
     6901 cggtgaatcg ccataagttt tgtgtcgatt atgatgacta catgcctgat tgtgcagcca
     6961 atcggctctt acactccgca ctagataagc ttcttagtct gcagctgtca tcagataatc
     7021 aacgctggct ttacgaactg cgttttgcgt ttgacggtat tccacttagc cgggatattg
     7081 aacgtgatat aaacaactta cgcctcgagc gtggtatggc tcattacaac gagccaatgg
     7141 catgggcgca attgatcctg aggggaatga gcccgagtgc tttgcaggga aacaccaaag
     7201 cgatatcact tttgttccct atggaagcgg tatttgaatc ctttgtggca cagaccctac
     7261 ccgacgagtt accacctcac ctcaaagttt tacctcaagc agcaacttat tctttggtaa
     7321 agcatggact taaagattgc tttaagcttc gccccgactt gctgattcaa tcacacaaac
     7381 cagttcaaac cgaaatggtg atggatacaa aatggaagct ggtgaatagc agccagcaga
     7441 caaaatcact ctatggactg gcacaagctg acttctatca aatgtttgcc tacggacaaa
     7501 aatatcttgg tgggaatggc gaaatgtatc tgatttaccc tgcgcatgat gactttagcc
     7561 aacctatagc gcagcatttt gctttctcgg agactttaaa attatgggtt gtgccatacc
     7621 ggataatggc aaaacgcggt gagaggatga tgtggccgaa tgacgtgtcc tgtacaatga
     7681 gccccaaact ggatcggatg gctgcttcag tatcctatag tggaggcatg agatgaccca
     7741 tgaataccag cgacaaagcc tggaacaact aataactcag caaacagcaa ctcccagaag
     7801 cgccgccgtt gtatttaatg agaaccaaac cctctggcaa acacttggct ttagccagtc
     7861 tcaagtttcg ctatggcttg cctcattgcc tcaaagtaat catctaaaag ggcccacaac
     7921 ggcaacgtac caggttatac ctgatatcgc ggcacatttg gtatcattgt tgcatcaagc
     7981 tggtgggcgc atgccccttg cacaagtact gaaaaagctc cctgctggta ttacgaccag
     8041 tgaacagcag atacgcaaac tggcacagaa gcacgcgcag atagagatta aaggcccctt
     8101 gctggtactg ataaattagc cggcatttgt agtaaccatt ggctgtatta aaggatttta
     8161 gaaaacatca tggcaaaaga agcaaaaaaa caagacccgc aacagcagct agaagcactg
     8221 ttcaataaac tggtcaaaca gcactccgag tttactcagc cattggtcga attaccggga
     8281 agtgcaggac gcttgcttaa acaggtccgt gatattgagc aagtggcgcc gctattccgt
     8341 gatgctggct tagtgaaagc ggtccccaag cccacaattg ttaatttacg cgtagccggt
     8401 attgcgacta aatcgtgctt agacaaggta ttgggtggtt ttatttatgc cagtgcagca
     8461 gtgcgcacag atttgttaat agatgacaat aaagtcagca cggtgggtag cgactcggta
     8521 tcagaactgg atgatattga ttttgaagct cagcgtaaac ggcttgattt aattgaaatt
     8581 cgtcaggctt atcagttagt tgagaaaaaa cttttaggtt atgagcctgg cgatttaatt
     8641 ttgctggata cgccgttgtt tttagcacgg gatatggctc cgctggagag aaatgtccgt
     8701 cataaacaag agtacgaaaa aaccagacaa gtgatagaaa ctttttggca aaaccatcgc
     8761 tccagaattt ttccctggaa tcaaaatggt gtagtacttg cgagcatttt ggctgagcgc
     8821 tttagtgcca ttgtaagtat cgccaaacaa gatttacgta ccgaacaagg acgcaaacaa
     8881 attttagcat ctgatgggtt ttctaaagag ttgatgcaaa atctggaagg actggatgaa
     8941 agtttggttg gcgtgggaga tttgcgattt atcaatggca ttttaggcaa ctattcgcgc
     9001 accatcgcat ttcgtttgtc tgaacaagaa aagcgcattg aacccaatat tgaagctaag
     9061 caaggtctaa ttggttttca cttccgtagt gcccgaggtg gacagataaa aatggtgcaa
     9121 ttagcgggtg acgaaccaga atggaaaagt gctgatctcg atttagttgc ctctcgcctg
     9181 atggtgctgg atatgcaaaa caaaggtaat gcaatgccgt tgccacaatt acttggttat
     9241 caacagcttg gtattttacc taaatttgct acgttttatc gccagggttt acaccaggca
     9301 ctaaagaata atgatattga ggcagggtgg ttggcaggcc tcgatgaggg attgaataat
     9361 ggcttataaa gttttaggta aattagtcgg taacactggt gatgccagtc agttaactat
     9421 ggttgtacaa gactcgtttt cagtacgccg tggtgagttt gtacgcatta tgcaccagga
     9481 acgcaaagat gaggggcaag tcgccgtact ggggcgagtg accaagataa gccgtactaa
     9541 tatgctctat aacgccggct ttggcgatgg tgtaaccgag cttgagcttt tgcccggtgc
     9601 gaatgtaact ggtgagaata tgtttgctca ggtcgagctg gttggttacc gcgatcctgt
     9661 tactcgtcaa atcaaaattc cacgtcgccc gcttaacccg ggtaccgctg ttgaaaccgt
     9721 cgattttcag tttttgagtg atttttacga atttaatgaa cactccagtt tgcacttagg
     9781 taacttagta ggctacgaca aaggtgagaa tacagttcct gtttttattg acgtgaacaa
     9841 gttggtcact gagcatttgg ctgttctggc aatgacgggt tcaggtaagt cttatacagt
     9901 tggccgcatt attgagcgtt tagttgcggt taataacggt acggtagtag tatttgaccc
     9961 acatggtgaa tacggtaaag ccctcgctgg cggtaatctg caattctcaa gcttcttaga
    10021 agccactgac gataagcgtg atcaagaagc actgccactg ataaaacaga cctttgaaaa
    10081 gctgcaagcc gccggtgcag gtattcaggt ttactcgccg cagcatgaat cttttaaaca
    10141 taaatacgcc agtaagaata cgccattagc gcttcagttt gaccattttg aaatggacga
    10201 tattgctgaa atattacctg gcttaacgga accgcagcaa cgagtactcg atgttgcaat
    10261 tcgttattgg cgctctgcag ataagactga gccacgagat attaaccgcc tacgcttctt
    10321 attaggtgat ggtattgaag agttaaaaga gtgggacgac ctttctgaag ctgaggcgaa
    10381 ggcgttatca ggccgtagtg ccgccgtcgc ctcgatgaag ctttcacgtg tgcttaatga
    10441 agctaaaagt ttttacagcg caacaatggc tgagccaact gatatttaca aaatggtggg
    10501 tcgcccgagc aatcaacagg ggcgtttagt cgttgttgac ttacaaggtt tgagtgatac
    10561 cgctaagcaa gtcatttgtg cactgttatc tagtgaaata ttaaaagcag cctcgagcaa
    10621 aacggacccg ctgcgcccat gctttatcat ttacgaagag ggtcataatt ttgccccggc
    10681 cggcggcaac gcagtcagtc accgcattat taaaaagatc gctggtgaag gtcgtaaatt
    10741 tggcgttgga tttggcattg tgagccagcg tccatcgaaa ttagattcag atgtgacctc
    10801 tcagtgcaat acgctaatta cgatgcgctt aaagaaccct gacgatcaac gatttatagc
    10861 taaagcatca gatatggtga gcaaagctga tctggaggaa ctcccgagtt tatcaaccgg
    10921 tgaagctttg gtgtgtggtc gttcaatccc tgcaccactg cttgttaaag ttggtagtaa
    10981 ggcattaatt cacggtggtg aatcaccaga ggtactgcgt gtttggggct ctttcagtgg
    11041 ctaaggtgcc tatgcctgaa gttgcctggg taaatgagac atttccactt ttagcaagcg
    11101 gtaagctatt ggaccaggat gtattggtcc acagcctggg ctgtagcgtt tggaatacat
    11161 tcgggcatga acagtcattt atggcggtag tggaatgccc ggctccaggg acatttggtg
    11221 ctgatattcg ctctgactcg ggctggtttc ataaatcttc tgcatcgcct gtgtgtttaa
    11281 tcgaatttga acgctttgat ggttcagcga aggggcagca aaagctggaa gaaaaattaa
    11341 aaaacttact ggaagcagca cagcgctgga atcattgccc caaaaccctg gtcctcagtg
    11401 cctggagcca aggcttagta ggtgtacctg atactcaaaa actgaaagac atatgccgta
    11461 tgggttttac ttcgtccacg ggtacccaag taatcgccgc tcctgatgtt gaggtggtat
    11521 ttagtcgctt tttatttatc aaaaacctga acatgattgt tttggacaga attcactatg
    11581 aggtgttgat gtgaatataa aatcagcaca ttttttgcca gggacgaata aacgccttgg
    11641 tgacaaatac ctgttacttg aatgccttgg tgatggcagc catggctggg tatggcgggc
    11701 ggaacgcctg aaagatggaa aaattgtcgc agtaaaaatc cctaaggata ttactcggga
    11761 agaccgtcag cttgccgaag gtaaagagct tttaaacgtc gagccgcacg aaaatattgt
    11821 gcaaattttt gatatgggcc gtatagataa tgaatggttc tatattgaaa tggagtattt
    11881 tcccagccaa accctggcgc aaaagctcga tgacaggcag cgtaattttg gccagaccta
    11941 tgaacggttg ttcagaatat atcgtcaagt gctctgtgct gtgcattatt tggccgaatt
    12001 accagtacca atatctcatg gagatattaa gccacataac attttggttg gagagcggga
    12061 cctggtgaag ttgacggact ttggcagctc agctttacca gaagaaattt acgttcgtac
    12121 ccgtgaaaat ggcgggactg tgctgtattc ggccccagag ttctccaacg tcgatagccg
    12181 tagaggcagc ctggaagagc tactattggg cgacatctat agcttaggtg ttttgctata
    12241 ccagctgctg acaggaaagc tgccccatga tacgccagca caagttcagc gccacgcacc
    12301 tttcaagctt ccgacagaga ttaatagctc tattcatacg gacctggaac aagtggttct
    12361 tacttgttta caaaaacgag cggaggatcg cttttccact atttccgact taattcatgc
    12421 atttgattct gctgccacta agcaattaaa agttggtgct attgctcccg tttttcaaac
    12481 taaacctgct caggactggt caagtgcggt acttgaagca atgtccgatc aatcttacca
    12541 aaaagctgct caattagcgt cgcaggagta tagccgcagt aaagatctac aagctttaca
    12601 tcagcaactt attgccttgt atcgcgccaa tcgcttattc gactttgaaa aagttgtaga
    12661 agataataaa gcgatcttac ttgagggtaa attgactcaa ccagcagctt tgttcgagtt
    12721 gatagttaag gcaaacctac agttacgcaa tatcagtcaa gcaaatttct ggctgttgca
    12781 acgaaagcaa gctgaagcag aaaacgcggc caccatgtat ttagaatcat ctatccttgg
    12841 ccttgaagcg aaatattctg aagcaagagt actattggag aaagtaaacc aaacaactcc
    12901 aatgaagttc catgtgttaa gccaactcat tttagtttgc gagcaaatgc gtgattactc
    12961 tggtgctgcg gcttatttga aagcggcatt gcgagtagca ccattggata acagcatgaa
    13021 agagaaaaaa gaattgtatg caaagcttgg agttatttga ttgaaaggat gaccattgcg
    13081 cgaacaaggc atagtggata tttaacactt ccgttcacat accggacaaa aaaataaagt
    13141 tttacataac gatttccttc ttgattgagt taagacaaaa gcctctcccc attagggcgg
    13201 aggcttctca gttataagcc tttaattaaa aaatagttaa tattcatata cttagataga
    13261 attatcgaaa agaactatta atctttgcat agtcaaatgg agtgatagcc gccattctat
    13321 tcgcatccag ataatcagcc caccactgca gcatcaacat tcgctcccca agatgttccg
    13381 ccttatggat ataagccgcc cgtactgagc tacgttcctg atgactcatc tgccgctcta
    13441 ctgcatccct cgaccacaat cccgattcaa tcaaagaact acacgccatc gtcctgaaac
    13501 catgtccaca cacttccgtt ttcgtgtcat agcccatcac tcgcaaggcc ttgttaagcg
    13561 tattctcact cattggctta cgcggatcgt gatcaccaac gaagatcagc tcacggtttc
    13621 cactcatgct tttgattttt tccagaatgg tgagagcttg tcgagataag ggtacaagat
    13681 gtggagtccg catcttcgaa ccacgctgag aatgcttaac tccctccagt ggctcacgtt
    13741 ctcccggaat cgtccacatg gcagtatcaa aatcaacttc tgaccagcga gcaaaacgca
    13801 actcgcttga acggatgaaa actaacaatg tgagttcaac cgccagtcgg gttaacggcc
    13861 tgccagaata gtgatagatg cgttgaagca attcaggaat gcggttaagt tccagagcag
    13921 cacgatgctg tcttttcgcc gtagcaaccg caccagcaat ctcttgagcg gggttgtagt
    13981 cgattaatcc actgtgtaca gcaaagcgta taatcgcggt agtatgctgc tgcaaacgag
    14041 cagcgacttc gagacgccca gacgactcca ccgctttgat ggggacaagc agatcccgag
    14101 tcttcagatc agcaatgttc cgcttaccga tagcagcaaa gagattatct tcgaggcttt
    14161 tcaatacacg agcgctatgc gatgcagacc acttctgatt gctggcgtgc cactctctgg
    14221 ctaccacttc aaacgttatc acctcttgct catgctctac cttaacagct ttcttgttct
    14281 cactaggatc gacgccgtta gctaacaact tacgggcttc atcccggcga cctctggcat
    14341 cgaccagcga gacttcaggg tatttcccca gcgccagcat cttctcctta ccgccaaaac
    14401 gataacgaag tcgccagtat ttggagccat tagggtgacc aacaaaacca tgccttcacc
    14461 gtcagtaagt ttataggctt ttgcttccgg cttagccgaa cggaccttca catcactcag
    14521 agccatgatg agtatccttt caagggccct gtgtgggtac aaacataatc gaaccgggaa
    14581 atacccgcag ttgtacccgc atcagtaagt tgatgtagat tgaatcaggt tgacttaggt
    14641 tgagtgaaaa agcgaggaaa gccttgcgga tactggattt caggcacaaa aaaagccgcc
    14701 cttgagcgac tcgatttgca tacgatgtgg tgccgaaggc cggactcgaa ccggcacgta
    14761 tttctacggt tgattttgaa tcaactgcgt ctaccgattt cgccacttcg gcactgaaga
    14821 ggtatgcgga aaacgttgtg gattatacct ggcgcacgcc accatgcaag cgccagcttg
    14881 ctcccacttc gctaagtgct gaaattttca gcattgcata cagcgatgtg taacctttgt
    14941 cacactccag gcaccccgcc ctgccatgct ctacacttcc cagacaacac cagagaagga
    15001 tcaaaaaatg tcgatgataa aatgctatgc cgcaaaagaa gcgggcggcg aactggaagt
    15061 ttatgagtac gatcccggtg aactgaagcc acaagatgtt gaagtgcagg tggattactg
    15121 cgggatctgc cattccgatc tgtcgatgat cgacaacgaa tggggatttt cacagtatcc
    15181 gctggttgcc gggcatgagg tgattggtcg cgtggtggcg ctcgggagcg ccgcgcagga
    15241 taaaggtttg caggtcggtc agcgtgtcgg gattggctgg acggcgcgta gctgcgggca
    15301 ttgcgacgcc tgtattagcg gtaatcagat caactgcgag caaggtgcgg tgccaacaat
    15361 tatgaatcgc ggtggctttg ccgagaagtt gcgtgcagac tggcaatggg tgattccact
    15421 gccagaaaat attgatatcg agtccgccgg gccgctgctg tgtggtggta tcacggtctt
    15481 taaaccactg ttgatgcacc atatcacagc caccagccgc gttggggtaa ttggtattgg
    15541 cgggctgggg catatcgcta taaaacttct gcacgcaatg ggatgcgagg tgacagcctt
    15601 tagttcgaat ccggcgaaag agcaggaagt actggcgatg ggtgccgata aagtggtgaa
    15661 tagccgcgat ccgcaggcac tgaaagcact ggcggggcag tttgatctca ttatcaacac
    15721 cgtcaacgtc agcctcgact ggcagcctta ttttgaggcg ctgacctatg gcggtaattt
    15781 ccatacggtc ggtgcggttc tcacgccgct gtctgttccg gcctttacgt taattgcggg
    15841 cgaccgcagc atctctggtt ctgctaccgg cacgccttat gagctgcgta agctgatgcg
    15901 ctttgccgcc cgcagcaagg ttgcaccgac caccgaattg ttcccgatgt cgaaaattaa
    15961 cgacgccatc aagcatgtgc gcgacggtaa ggcgcgttac cgcgtggttc tgaaagccga
    16021 cttctgatcc ctttgccggg tgcctgtcgc ccggcactaa aatctctttg gaaggcatct
    16081 cgcaatctgc tgaatttagc ggtgaagagt gcgcagccgt tgcctatact ccaggcagga
    16141 aactggagga aatctcatga gcgaacccct gttaattgcc cgcacgccag acacagaact
    16201 gtttttactg ccgggaatgg ctaaccgtca cgggctgatt actggcgcaa cggggacggg
    16261 taaaactgtc acgctgcaaa aactggcgga gtctctatcg gaaatcggtg ttccggtgtt
    16321 tatggccgat gtgaaaggcg atctgaccgg cgtcgcgcag gcaggaacgg cgtcggaaaa
    16381 actgctcgca aggcttaaaa atatcggcgt caatgactgg caaccgcatg ctaatccggt
    16441 ggtggtgtgg gatatctttg gcgagaaagg ccatccggtg cgggcgacgg tttcggatct
    16501 ggggccgctg ttgctggcgc ggctgttgaa tctcaacgat gtgcaatctg gcgtgctgaa
    16561 tatcatcttc cgtattgctg acgatcaggg attgttgctg ctcgacttta aagatctgcg
    16621 ggcgattacc cagtacatcg gcgataacgc caaatctttc cagaatcagt acggtaatat
    16681 cagtagcgca tcggttggtg ccatccagcg cggactgttg tcgctggaac agcaaggcgc
    16741 agcacacttc tttggcgagc cgatgctgga tatcaaagac tggatgcgca ccgataccaa
    16801 cggtaaaggc gttatcaata tcctcagcgc cgagaagctt tatcagatgc cgaaactgta
    16861 cgccgccagc ctgctgtgga tgctttcaga gttgtatgaa caattgccgg aagcgggcga
    16921 tctggagaaa ccaaaactgg tgtttttctt cgacgaggca catctgctgt ttaacgatgc
    16981 accgcaggta ctgctggata agattgagca ggtgatacgg cttattcgct caaaaggcgt
    17041 aggcgtctgg ttcgtttcgc aaaacccgtc tgatattccg gataatgtgc tcgggcagct
    17101 cggtaatcgc gttcaacacg ctttgcgtgc ttttacgccc aaagatcaga aagcggtaaa
    17161 agctgcggcg caaaccatgc gggccaatcc ggcatttgat accgaaaagg caatccagga
    17221 actggggacc ggcgaggcgt taatctcgtt tcttgatgtg aaaggaagtc cttcagtggt
    17281 ggaacgggcg atggtgattg cgccttgttc gcggatggga ccggtgacgg aagatgagcg
    17341 taatggcttg attaatcact ctccggtgta tggcaagtac gaggatgagg tggaccggga
    17401 atccgcctat gagatgttgc aaaaaggcgt acaggcaagc gccgagcagc aaaataatcc
    17461 ccccgcgaaa gggaaagagg tggcggtgga tgacggcatt cttggtggat taaaggatat
    17521 tttgtttggc actaccggac cacgcggcgg gaagaaagat ggtgtggtgc aaacaatggc
    17581 gaaaagcgcc gcccgccagg tgacgaatca gattgtacgc gggatgttgg ggagtttgct
    17641 ggggggaaga agaaggtaag tcaggtgcgg tctgtgcccc tcaccctaac cctctcccca
    17701 aaggggcgag gggaccgatc gaactcgtat ttgactttat caacaatgtg aaagggagct
    17761 ttcgctccct tgcttggtta cgattttctc attaacagcc acaggctgat taagaagaag
    17821 ctggcgcttg gcaacagtgc gccgatgatc ggcgggatgc cataaaccaa cgtcaacggg
    17881 ccgaagatct ggtccagtac gtagaagaca aaaccgaagc tgataccggt gaccacacgc
    17941 acgcccatcg gtacgctacg cagtgggcca aagatgaacg acagcgccat cagcatcatc
    18001 accgccacgg atagcggctg gaagattttg ctccacatgt tgagctgata acgtccagcg
    18061 tcctgaccgc tcgacttcag atacttcacg tagttgtgca aaccgctgat tgagagtgca
    18121 tccgggtcca gcgccaccac gcccagtttg tctggcgtga ggttggtttt ccaggtgccg
    18181 ctcaccgtct gcgaaccggt aatctgtttc ggattggtca gatcagattc atcaacctgc
    18241 gacagacgcc agactttatg ttccgggtca aacttcgcag tagcggcata gcgtacggat
    18301 tgcagacgac gattctcgtt aaaggcataa atgctgatgc cacctaactc ttcgtcacct
    18361 ttaacccgct caatgtagac gaagttgttg ccatctttcg cccataagcc ttgctgggta
    18421 gagagcaacg agccgccgta catcgcctgc gcacggtagt tacgcgccat ctgctcgccc
    18481 tgcggcgcga cccattcgcc aatcgccatc gtcagcaaga ccagcggaat ggcggttttc
    18541 atcaccgaca gcgccacctg catacgggta aaaccagaag cctgcatcac caccagttcg
    18601 ctgcgctgcg ccagcatccc aagaccaagc aacgccccaa gcagagccgc catcgggaag
    18661 aagatttgca catctttcgg cacgctcagc aaggtataca ttcctgcgcc taacgcgtcg
    18721 taactcccct gcccggcttt tttcagctga tcgacaaact tgataatgcc cgacagcgac
    18781 accagcatga acagcgtcat catgatggtg gtgaaaatag ttttaccgat atagcggtca
    18841 agtacgccaa aaggttgcat cacaccgctc ctttacgcga aaaactggcg cgcaggcggc
    18901 ggaccggcac ggtgtcccaa aggttgagaa caatcgccaa agccagataa atcaggttaa
    18961 cggtccacat ccacagcgtc gggtccagct tacctttacc gccgttcgat ttcagggagg
    19021 tctggatcag gaagaacaac agatacagca gcatggctgg cagcatcgat agtacgcgcc
    19081 cctgacgcgg gttaaccacg ctcagcggta cgaccataag tgccatcata aacacggtga
    19141 acaccaacgt gatacgccag ttcagttctg cgcgagcacg atcggtgtca gtgttccaca
    19201 atgtgcgcat gtccatctgg tcggtatcgt tcgggtcgag cgccaccgcc tggtgaccaa
    19261 tgatcgcctg ataatcctgg aaatccgtaa tgcggaaatc acgtaacagt gcagtgcctt
    19321 cgaagcgcgt tccctggttg agagtgacga cctgggagcc gtcgcgcagc tgggttaaat
    19381 gtccggaatc ggccaccacc acagaaggac gtgcattacc ttttggtcga atttgcgcga
    19441 ggaacacatc tttgaaatcg ctgccgtcaa cgctttcgat gaacagcacc gaactgccat
    19501 tagtcgcttg ctggaattgc ccttgcgcca gcgccgccat gccagggttc gctttcgctt
    19561 ctgctaacac ttcatcctga tgacgcgatg accacggtcc cgcccacatc acgttaaccg
    19621 ccgcgacgat tgccgtgaat accgcaagga tcattgccgc tttcaccaga accgctttgc
    19681 tcaggccgca ggcatgcatt accgtaattt cactttcggt atacagtttg cccagcgtca
    19741 tcagcagccc gaggaacagg cttaatggca ggataagctg cgccatttcc ggcacgccca
    19801 acccgagaag ggagagcacc agattcgccg gaatatcgcc gtcaaccgct gcgccgagga
    19861 tcctcactaa cttttgacag aagaagatca aaagcaagat gaagagtatc gccagctggc
    19921 ttttgagcgt ctcccgcacc agatatctta tgattatcac tttaaatacg cccgtaaaaa
    19981 ctcgtctttt gcaggatttt agcttgtttc atggcttaaa cgtcatttat tctcttgagt
    20041 cgtcgaaatc gtcgctaaga taattatact caacggattc acctctcaga ttttgttctg
    20101 acgtgccaat gccgtaataa cgttaagatt aacacgaagt catcgcaaca gcggacatga
    20161 gttacgaaag cttgcaattc tatctgtagc caccgccgtt gtctttaaga ttcaggagcg
    20221 tagtgcatgg agtttagtgt aaaaagcggt agcccggaga aacagcggag tgcctgcatc
    20281 gtcgtgggcg tcttcgaacc acgtcgcctt tctccgattg cagaacagct cgataaaatc
    20341 agcgatgggt acatcagcgc cctgctacgt cggggcgaac tggaaggaaa accggggcag
    20401 acattgttgc tgcaccatgt tccgaatgta ctttccgagc gaattctcct tattggttgc
    20461 ggcaaagaac gtgagctgga tgagcgtcag tacaagcagg ttattcagaa aaccattaat
    20521 acgctgaatg atactggctc aatggaagcg gtctgctttc tgactgaact gcacgttaaa
    20581 ggccgtaaca actactggaa agtgcgtcag gctgtcgaga cggcaaaaga gacgctctac
    20641 agtttcgatc agctgaaaac gaacaagagc gaaccgcgtc gtccgctgcg taaaatggtg
    20701 ttcaacgtgc cgacccgccg tgaactgacc agcggtgagc gcgcgatcca gcacggtctg
    20761 gcgattgccg ccgggattaa agcagcaaaa gatctcggca atatgccgcc gaatatctgt
    20821 aacgccgctt acctcgcctc tcaggcgcgc caactggctg acagctacag caagaatgtc
    20881 attacccgcg ttatcggcga acaacagatg aaagagctgg ggatgcattc ctatctggcg
    20941 gtcggtcagg gttcgcagaa cgaatcgctg atgtcggtga ttgagtacaa aggcaacgcg
    21001 tcggaagatg cacgcccaat cgtgctggtg ggtaaaggtt taaccttcga ctccggcggt
    21061 atctcgatca agccttcaga aggcatggat gagatgaagt acgatatgtg cggtgcggca
    21121 gcggtttacg gcgtgatgcg gatggtcgcg gagctacaac tgccgattaa cgttatcggc
    21181 gtgctggctg gctgcgaaaa catgcctggc gggcgtgcct atcgtccggg cgatgtgtta
    21241 accaccatgt ccggtcaaac cgttgaagtg ctgaacaccg acgctgaagg ccgcctggta
    21301 ctgtgcgacg tgttaactta cgttgagcgt tttgagccgg aagcggtgat tgacgtggcg
    21361 acgctgaccg gtgcctgcgt gatcgcgctg ggtcatcaca ttactggtct gatggcgaac
    21421 cacaatccgc tggcccatga actgattgcc gcgtctgaac aatccggtga ccgcgcatgg
    21481 cgcttaccgc tgggtgacga gtatcaggaa caactggagt ccaattttgc cgatatggcg
    21541 aacattggcg gtcgtcctgg tggggcgatt accgcaggtt gcttcctgtc acgctttacc
    21601 cgtaagtaca actgggcgca cctggatatc gccggtaccg cctggcgttc tggtaaagcg
    21661 aaaggcgcaa ccggtcgtcc ggtagcgttg ctggcacagt tcctgttaaa ccgcgccggg
    21721 tttaacggcg aagagtaagt ttgcgtttta ccctcaccct aaccctctcc ctaagggaga
    21781 gggaaccgat cgagcgcaat agaaattcat tgctcatctt ttagggtgag gaatgcagca
    21841 atatttaaat ccaccacaag aagccccatt tatgaaaaac gcgacgttct accttctgga
    21901 caatgacacc accgtcgatg gcttaagcgc cgttgagcag ctggtgtgtg aaattgccgc
    21961 agaacgttgg cgcagtggta agcgcgtgct catcgcctgt gaagatgaaa agcaggctta
    22021 ccggctggat gaagccctgt gggcgcgtcc ggcagaaagc tttgttccgc ataatttagc
    22081 gggagaagga ccgcgcggcg gtgcgccggt ggagatcgcc tggccgcaaa aacgcagcag
    22141 cagcccgcgc gatatattga ttagtctgcg aacaagcttt gcagattttg ccaccgcttt
    22201 cacagaagtg gtagacttcg ttccttatga agattctctg aaacaactgg cgcgcgaacg
    22261 ctataaagcc taccgcgtgg ctggtttcaa cctgaatacg gcaacctgga aataatggaa
    22321 aagacatata acccacaaga tatcgaacag ccgctttacg agcactggga aaagcagggc
    22381 tactttaagc ctaatggcga tgaaagccag gaaagtttct gcatcatgat cccgccgccg
    22441 aacgtcaccg gcagtttgca tatgggtcac gccttccagc aaaccatcat ggataccatg
    22501 atccgctatc agcgcatgca gggcaaaaac accctgtggc aggtcggtac tgaccacgca
    22561 gggatcgcta cccagatggt cgttgagcgc aagattgccg cagaagaagg taaaacccgt
    22621 cacgactacg gccgcgaagc tttcatcgac aaaatctggg aatggaaagc ggaatccggc
    22681 ggcaccatta cccgtcagat gcgccgtctc ggcaactccg tggactggga gcgtgaacgc
    22741 ttcaccatgg acgaaggcct gtccaatgcg gtgaaagaag ttttcgttcg tctgtataaa
    22801 gaagacctga tttaccgtgg caaacgcctg gtaaactggg atccgaaact gcgcaccgct
    22861 atctctgacc tggaagtgga aaaccgcgaa tcgaaaggtt cgatgtggca catccgctat
    22921 ccgctggctg acggcgcgaa aaccgcagat ggtaaagatt atctggtggt tgcgactact
    22981 cgtccggaaa ccctgctggg cgataccggc gtggccgtta acccggaaga tccgcgttac
    23041 aaagatctga ttggcaaata tgtcattctg ccgctggtta accgtcgtat tccgatcgtt
    23101 ggcgacgaac acgccgacat ggaaaaaggc accggctgcg tgaagatcac cccggcgcac
    23161 gactttaacg actatgaagt cggtaaacgt cacgccctgc cgatgatcaa catcctgacc
    23221 tttgacggcg atatccgtga aagtgcccag gtgttcgata ccaaaggtaa cgaatctgac
    23281 gtttattcca gcgagatccc cgcagaattc cagaaactgg agcgttttgc tgcacgtaaa
    23341 gcggtcgttg ctgcggttga cgcactcggc ctgctggaag aaattaaacc acatgacctg
    23401 accgttcctt atggcgaccg tggtggcgtg gtcatcgaac cgatgctgac cgaccagtgg
    23461 tacgtgcgtg ccgatgtcct ggcgaaaccg gcagttgaag cggttgagaa cggcgacatt
    23521 cagttcgtac cgaagcagta cgaaaacatg tacttctcct ggatgcgcga tattcaggac
    23581 tggtgtatct ctcgtcagtt gtggtggggt caccgtatcc cggcatggta tgacgaagcg
    23641 ggtaacgttt atgttggccg caacgaagac gaagtgcgta aagaaaataa cctcggtgct
    23701 gatgttgtcc tgcgtcagga cgaagacgtt ctcgacacct ggttctcttc tgcgctgtgg
    23761 accttctcta cccttggctg gccggaaaat accgacgccc tgcgtcagtt ccacccaacc
    23821 agcgtgatgg tatctggttt cgacatcatc ttcttctgga ttgcccgcat gatcatgatg
    23881 accatgcact tcatcaaaga tgaaaatggc aaaccgcagg tgccgttcca cactgtttac
    23941 atgaccggcc tgattcgtga cgacgaaggc cagaagatgt ccaaatccaa gggtaacgtt
    24001 attgacccac tggatatggt tgacggtatt tcgctgccag aactgctgga aaaacgcacc
    24061 ggcaatatga tgcagccaca gctggcggac aaaatccgta agcgcaccga gaagcagttc
    24121 ccgaacggta ttgaaccgca cggtactgac gcgctgcgct tcaccctggc ggcgctggct
    24181 tctaccggtc gcgatatcaa ctgggatatg aaacgcctgg aaggttaccg taacttctgt
    24241 aacaagctgt ggaacgccag ccgctttgtg ctgatgaaca cagaaggtca ggattgcggc
    24301 ttcaacggcg gcgaaatgac gctgtcgctg gcggatcgct ggattctggc ggagttcaac
    24361 cagaccatca aagcgtaccg cgaagcgctg gacagcttcc gcttcgatat cgccgcaggc
    24421 attctgtatg agttcacctg gaaccagttc tgtgactggt atctggaact gactaagccg
    24481 gtaatgaacg gtggcaccga agccgaactg cgcggtactc gccatacgct ggtgaccgtg
    24541 ctggaaggtc tgctgcgcct cgcgcatccg atcattccgt tcatcaccga aaccatctgg
    24601 cagcgtgtga aagtactttg cggtatcact gccgacacca tcatgctgca gccgttcccg
    24661 cagtacgatg catctcaggt tgatgaagcc gcactggccg acacagagtg gctgaagcag
    24721 gcgatcgttg cggtacgtaa catccgtgca gaaatgaaca tcgcgccggg caaaccgctg
    24781 gagctgctac tgcgtggttg cagcgcggat gcagaacgtc gcgtaaatga aaaccgtggc
    24841 ttcctgcaaa cgctggcgcg tctggaaagc atcaccgtgc tgcctgccga tgacaaaggt
    24901 ccggtttccg ttactaagat catcgacggc gcagagctgc tgatcccgat ggctggcctc
    24961 atcaacaaag aagatgagct ggcgcgtctg gcgaaagaag tggcgaagat cgaaggcgaa
    25021 atcagccgta tcgagaacaa gctggcgaat gaaggctttg tcgcccgcgc accggaagcg
    25081 gtcatcgcga aagagcgtga aaagctggaa ggctatgcag aagcgaaagc gaagctgatt
    25141 gaacagcagg ctgttatcgc cgcgctgtaa tcaaaacaca gtaatgaaaa cgaaggccgg
    25201 agcatgctcc ggccttttta tctcttacag aaatggtaaa gaaggcataa ttccccgtga
    25261 aatcctggct aagaagcctt tatctggttg ttcttcttta ttgattaaag gaaggtcatc
    25321 caaatcgccc agcaaatacg tattttttgc ctgccagtca atcatccata ttttcagcag
    25381 tggataagcc agaccgcccg taatcccgga tatcaccacc aacgcacaca tgcgataaag
    25441 cataccgtgg tacgttaaag ttgagcgaaa acgaatacgc ccatcattca gtgacaggtt
    25501 gctcataaaa tgatttcgca acgacaccgt taaataactg gtgctaacag caatcccaaa
    25561 ataatagatt aactgcgcga ttatcatttt acgttgcatt tccataaatt gctgtaaatt
    25621 ctcaatatca tcatttgcat acacagaact atcatacgca tttaatattt gatcgatgat
    25681 aatataacca gcaacagcga gaaaaggtaa taaagctaaa aatgcgagaa tggcatattt
    25741 tatacaatac gtagtatcta atttcacctt gaaacgatgt ataccgaaac tggtattgct
    25801 ccagagaaaa ctcattacca ggctatataa agtaccatta aaaataccaa tggaaacaat
    25861 accaactatt gccatcagaa ccacagatat aataacacta cttgaactat tggcatgtag
    25921 catctttgtc gagataaaga aaacagtccc catcccaatg gccattaaaa tcggcaagaa
    25981 aaaggttacc caccagaacc ctttcataga gcaattaaaa ctaaatctta cgccgttgag
    26041 actggtcatc aaggcctgat aacgcagtcc ttttgctgcc ataaatataa gcaaaaccaa
    26101 cagtaacaaa gtcaaaacac agccaacaag tggcatatct gctgacactg tcattaagat
    26161 tgcgaaatag caaaaaacaa aaacaagaca actaacaaaa acattcccac cagtaattcc
    26221 ataagaaaat cgttgtccgt taacttccat attagcatag agataacgct tacatttcat
    26281 taatgcccat ggcaaataga tacctagcgt tataatcgtt aacaacacat tcaccacaca
    26341 aataagaaaa tatctctcac ctgtaccatg aaaaacaaac gaatgggacg gaacatccat
    26401 ttcattaata acttgagcca taaaaattcc ctgaatta
//
LOCUS       DAPHDW010000057        26149 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.057, whole genome shotgun sequence.
ACCESSION   DAPHDW010000057 DAPHDW010000000
VERSION     DAPHDW010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 26149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26149
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.057"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1305
                     /gene="pgaA"
                     /locus_tag="RH057_002343"
     CDS             <1..1305
                     /gene="pgaA"
                     /locus_tag="RH057_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDV3128700.1"
                     /translation="VTQHTINTSPPFLRLMGTPTSIPNDTWLQGHSFLSTVAKYSNDL
                     PQAEMTARELAYNAPGNQGLRIDYASVLQARGWPRAAENELKKAEVIEPRNINLEVEQ
                     AWTALTLQEWQQAVVLTHDVVEREPQDPGVVRLKRAVDVHNLAELRIAGSTGIDAEGP
                     DSGKHDVDLTTIVYSPPLKDNWRGFAGFGYADGQFSEGKGIVRDWLAGVEWRSRNIWL
                     EAEYAERVFNHEHKPGARLSGWYDFNDNWRIGSQLERLSHRVPLRAMKNGVTGNSAQA
                     YVRWYQNERRKYGVSWAFTDFSDSNQRHEVSLEGQERIWSSPYLIVDFLPSLYYEQNT
                     EHDTPYYNPIKTFDIVPAFEASHLLWRSYENSWEQIFSAGVGASWQKHYGTDVVTQLG
                     YGQRISWNDVIDAGATLRWEKRPYDGDREHNLYVEFDMTFRF"
     gene            1314..3332
                     /gene="pgaB"
                     /locus_tag="RH057_002344"
     CDS             1314..3332
                     /gene="pgaB"
                     /locus_tag="RH057_002344"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     N-deacetylase PgaB"
                     /protein_id="HDV3128701.1"
                     /translation="MLRNGNKYLLMLVSIIMLTACISQSRTSFIPPQDRESLLAEQPW
                     PHNGFVAISWHNVEDEAADQRFMSVRTSALREQFAWLRENGYQPVSIAQIREAHRGGK
                     PLPEKAVVLTFDDGYQSFYTRVFPILQAFQWPAVWAPVGSWVDTPADKQVKFGDELVD
                     REYFATWQQVREVARSRLVELASHTWNSHYGIQANATGSLLPVYVNRAYFTDHARYET
                     AAEYRERIRLDAVKMTEYLRTKVEVNPHVFVWPYGEANGIAIEELKKLGYDMFFTLES
                     GLANASQLDSIPRVLIANNPSLKEFAQQIITVQEKSPQRIMHIDLDYVYDENLQQMDR
                     NIDVLIQRVKDMQISTVYLQAFADPDGDGLVKEVWFPNRLLPMKADIFSRVAWQLRTR
                     SGVNIYAWMPVLSWDLDPTLTRVKYLPTGEKKAQIHPEQYHRLSPFDDRVRAQVGMLY
                     EDLAGHAAFDGILFHDDALLSDYEDASAPAITAYQQAGFSGSLSEIRQNPEQFKQWAR
                     FKSRALTDFTLELSARVKAIRGPHIKTARNIFALPVIQPESEAWFAQNYADFLKSYDW
                     TAIMAMPYLEGVAEKSADQWLIQLTNQIKNIPQAKDKSILELQAQNWQKNGQHQAISS
                     QQLAHWMSLLQLNGVKNYGYYPDNFLHNQPEIDLIRPEFSTAWYPKND"
     gene            3325..4650
                     /gene="pgaC"
                     /locus_tag="RH057_002345"
     CDS             3325..4650
                     /gene="pgaC"
                     /locus_tag="RH057_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HDV3128702.1"
                     /translation="MINRIVSFFILCLVLCIPLCVAYFHSGELMMRFVFFWPFFMSIM
                     WIVGGVYFWVYRERHWPWGENAPAPQLKDNPSISIIIPCFNEEKNVEETIHAALAQRY
                     ENIEVIAVNDGSTDKTRAILDRMAAQIPHLRVIHLAQNQGKAIALKTGAAAAKSEYLV
                     CIDGDALLDRDAAAYIVEPMLYNPRVGAVTGNPRIRTRSTLVGKIQVGEYSSIIGLIK
                     RTQRIYGNVFTVSGVIAAFRRSALAEVGYWSDDMITEDIDISWKLQLNQWTIFYEPRA
                     LCWILMPETLKGLWKQRLRWAQGGAEVFLKNMTRLWRKENFRMWPLFFEYCLTTIWAF
                     TCLVGFIIYAVQLAGVPLNIELTHIAATHTAGILLCTLCLLQFIVSLMIENRYEHNLT
                     SSLFWIIWFPVIFWMLSLATTLVSFTRVMLMPKKQRARWVSPDRGILRG"
     gene            4652..5065
                     /gene="pgaD"
                     /locus_tag="RH057_002346"
     CDS             4652..5065
                     /gene="pgaD"
                     /locus_tag="RH057_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6-N-acetyl-D-glucosamine
                     biosynthesis protein PgaD"
                     /protein_id="HDV3128703.1"
                     /translation="MNNLIITTRQSPVRLLVDYVATTILWTLFALFIFLFAMDLLTGY
                     YWQSEARSRLQFYFLLAVANAVVLIVWALYNKLRFQKQQHHAAYQYTPQEYAESLAIP
                     DELYQQLQKSHRMSVHFTSQGQIKMVVSEKALVRA"
     gene            complement(5115..5903)
                     /gene="phoH"
                     /locus_tag="RH057_002347"
     CDS             complement(5115..5903)
                     /gene="phoH"
                     /locus_tag="RH057_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HDV3128704.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMGGVEA
                     IGMARDSRDTSPILARNEAQLHYLKAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDVLVRRLGASFMQYCLRPEIG
                     KVEIAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(6522..7793)
                     /gene="efeB"
                     /locus_tag="RH057_002348"
     CDS             complement(6522..7793)
                     /gene="efeB"
                     /locus_tag="RH057_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HDV3128705.1"
                     /translation="MQYEDKNGVNEPSRRRLLKGIGALALAGSCPVAHAQKTQSAPGT
                     LSPVARNEKQPFYGKHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLT
                     QGGAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKM
                     TRFPNDSLDAALCHGDVLLQICANTQDTVIHALRDIIKHTPDLLSVRWKREGFISDHA
                     ARSKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQ
                     FRVEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLAN
                     PRTAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALE
                     EYVKPIGGGYFFALPGVKDANDYLGSALLRV"
     gene            complement(7799..8926)
                     /gene="efeO"
                     /locus_tag="RH057_002349"
     CDS             complement(7799..8926)
                     /gene="efeO"
                     /locus_tag="RH057_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HDV3128706.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVTQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYADQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(8984..9814)
                     /locus_tag="RH057_002350"
     CDS             complement(8984..9814)
                     /locus_tag="RH057_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HDV3128707.1"
                     /translation="MFVPFLIMLREGLEAALIVSLIASYLKRTQRGRWIGVMWIGVLL
                     AAALCLGLGIFINETTGEFPQKEQELFEGIVAVIAVVILTWMVFWMRKVSRNVKVQLE
                     QAVDSALQRGNHHGWALVMMVFFAVAREGLESVFFLLAAFQQDVGIWPPLGAMLGLAT
                     AVVLGFLLYWGGIRLNLGAFFKWTSLFILFVAAGLAAGAIRAFHEAGLWNHFQEIAFD
                     MSAVLSTHSLFGTLMEGFFGYQEAPSVSEVAVWFIYLIPALVAFALPPRAGATASRSA
                     "
     gene            complement(10356..11864)
                     /gene="putP"
                     /locus_tag="RH057_002351"
     CDS             complement(10356..11864)
                     /gene="putP"
                     /locus_tag="RH057_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HDV3128708.1"
                     /translation="MAISTPMLVTFCVYIFGMILIGFIAWRSTKNFDDYILGGRSLGP
                     FVTALSAGASDMSGWLLMGLPGAVFLSGISESWIAIGLTLGAWINWKLVAGRLRVHTE
                     YNNNALTLPDYFTGRFEDKSRILRIISALVILLFFTIYCASGIVAGARLFESTFGMSY
                     ETALWAGAAATILYTFIGGFLAVSWTDTVQASLMIFALILTPVIVIISVGGFGVSLEV
                     IKQKSIENVDMLKGLNFVAIISLMGWGLGYFGQPHILARFMAADSHHSIVHARSISMT
                     WMILCLAGAVAVGFFGIAYFNEHPAVAGAVNQNAERVFIELAQILFNPWIAGILLSAI
                     LAAVMSTLSCQLLVCSSAITEDLYKAFLRKHASQKELVWVGRVMVLVVALVAIALAAN
                     PENRVLGLVSYAWAGFGAAFGPVVLFSVMWSRMTRNGALAGMIIGALTVIVWKQFGWL
                     GLYEIIPGFIFGSIGIVVFSLLGKAPSAAMQKRFAEADAHYHSAPPSRLQES"
     gene            complement(12023..12232)
                     /locus_tag="RH057_002352"
     CDS             complement(12023..12232)
                     /locus_tag="RH057_002352"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128709.1"
                     /translation="MMLQLCATMLNVTCVASLKMNEMLIKEIDMTGIKKITQTFSLRQ
                     LTFLKGATAKNVRECNLMKNSVAEH"
     gene            12287..16249
                     /gene="putA"
                     /locus_tag="RH057_002353"
     CDS             12287..16249
                     /gene="putA"
                     /locus_tag="RH057_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3128710.1"
                     /translation="MGTTTMGVKLDDATRERIKSAATRIDRTPHWLIKQAIFSYLEQL
                     ENSDTLPELPALLSGAANESDEAPTPAEEPHQPFLDFAEQILPQSVSRAAITAAYRRP
                     ETEAVSMLLEQARLPQPVAEQAHKLAYQLADKLRNQKNASGRAGMVQGLLQEFSLSSQ
                     EGVALMCLAEALLRIPDKATRDALIRDKISNGNWQSHIGRSPSLFVNAATWGLLFTGK
                     LVSTHNEASLSRSLNRIIGKSGEPLIRKGVDMAMRLMGEQFVTGETIAEALANARKLE
                     EKGFRYSYDMLGEAALTAADAQAYMVSYQQAIHAIGKASNGRGIYEGPGISIKLSALH
                     PRYSRAQYDRVMEELYPRLKSLTLLARQYDIGINIDAEEADRLEISLDLLEKLCFEPE
                     LAGWNGIGFVIQAYQKRCPLVIDYLIDLATRSRRRLMIRLVKGAYWDSEIKRAQMDGL
                     EGYPVYTRKVYTDVSYLACAKKLLAVPNLIYPQFATHNAHTLAAIYQLAGQNYYPGQY
                     EFQCLHGMGEPLYEQVTGKVADGKLNRPCRIYAPVGTHETLLAYLVRRLLENGANTSF
                     VNRIADTSLPLDELVADPVTAVEKLAQQEGQTGLPHPKIPLPRDLYGHGRDNSAGLDL
                     ANEHRLASLSSALLNSALQKWQALPMLEQPVAAGEMSPVINPAEPKDIVGFVREATPR
                     EVEQALESAVNNAPIWFATPPAERAAILHRAAVLMESQMQQLIGILVREAGKTFSNAI
                     AEVREAVDFLHYYAGQVRDDFANETHRPLGPVVCISPWNFPLAIFTGQIAAALAAGNS
                     VLAKPAEQTPLIAAQGIAILLEAGVPPGVVQLLPGQGETVGAQLTGDDRVRGVMFTGS
                     TEVATLLQRNIASRLDAQGRPIPLIAETGGMNAMIVDSSALTEQVVIDVLASAFDSAG
                     QRCSALRVLCLQDEIADHTLKMLRGAMAECRMGNPGRLTTDIGPVIDSEAKANIERHI
                     QTMRSKGRPVFQAVRENSEDAREWQSGTFVAPTLIELDDFAELQKEVFGPVLHVVRYN
                     RNQLPELIEQINASGYGLTLGVHTRIDETIAQVTGSAHVGNLYVNRNMVGAVVGVQPF
                     GGEGLSGTGPKAGGPLYLYRLLANRPESALAVTLARQDAEYPVDAQLKAALTQPLNAL
                     REWAANRPELQALCTQYGELAQAGTQRLLPGPTGERNTWTLLPRERVLCIADDEQDAL
                     TQLAAVLAVGSQVLWPDDALHRQLVKALPSAVSERIQLAKAENITAQPFDAVIFHGDS
                     DQLRALCEAVAARDGAIVSVQGFARGESNILLERLYIERSLSVNTAAAGGNASLMTIG
                     "
     gene            complement(16289..16927)
                     /gene="rutR"
                     /locus_tag="RH057_002354"
     CDS             complement(16289..16927)
                     /gene="rutR"
                     /locus_tag="RH057_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HDV3128711.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEIFFNQTVENVQRIIIEGIRPR"
     gene            17215..18306
                     /gene="rutA"
                     /locus_tag="RH057_002355"
     CDS             17215..18306
                     /gene="rutA"
                     /locus_tag="RH057_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HDV3128712.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIVQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSVPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQAAEQTGRDVGSYVLFMVIADETDDAARAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLPALTQEVA"
     gene            18306..18998
                     /gene="rutB"
                     /locus_tag="RH057_002356"
     CDS             18306..18998
                     /gene="rutB"
                     /locus_tag="RH057_002356"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HDV3128713.1"
                     /translation="MTTLTARPEAITFDPQQSALIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEYFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            19010..19396
                     /gene="rutC"
                     /locus_tag="RH057_002357"
     CDS             19010..19396
                     /gene="rutC"
                     /locus_tag="RH057_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HDV3128714.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFCIQCGLVKPDALVEIATIAHIAK"
     gene            19404..20204
                     /gene="rutD"
                     /locus_tag="RH057_002358"
     CDS             19404..20204
                     /gene="rutD"
                     /locus_tag="RH057_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HDV3128715.1"
                     /translation="MKLSLSPPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIEHYAVVGHALGALVGMQLALDYP
                     ASVTVLISVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPANWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPSACSS
                     ELHAALPDSQKMVMRYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            20214..20804
                     /locus_tag="RH057_002359"
     CDS             20214..20804
                     /locus_tag="RH057_002359"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HDV3128716.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLRELYALMKWGP
                     TSANCSPARIVFIRTVEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQYVDDAFFA
                     GSTLKSNLLINIGYGDSSKLYARLPRLSFEEACGLL"
     gene            20815..21309
                     /gene="rutF"
                     /locus_tag="RH057_002360"
     CDS             20815..21309
                     /gene="rutF"
                     /locus_tag="RH057_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309280.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HDV3128717.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHATPYGLVWFDRSYHALM
                     RPAC"
     gene            21330..22658
                     /gene="rutG"
                     /locus_tag="RH057_002361"
     CDS             21330..22658
                     /gene="rutG"
                     /locus_tag="RH057_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HDV3128718.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPYERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSHAAWFGLPHFSTPAFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAPVIGGASIVVFGLIAVAGARIWVQNRVDLSQNGNLIMVAVTL
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSKRLVDVPPPEVVHQEP"
     gene            complement(22741..22914)
                     /locus_tag="RH057_002362"
     CDS             complement(22741..22914)
                     /locus_tag="RH057_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV3128719.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGQHSGGNFKNDPQRASEAG
                     KKGGKSSHGKSDN"
     gene            23287..23883
                     /gene="wrbA"
                     /locus_tag="RH057_002363"
     CDS             23287..23883
                     /gene="wrbA"
                     /locus_tag="RH057_002363"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HDV3128720.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            23904..24131
                     /locus_tag="RH057_002364"
     CDS             23904..24131
                     /locus_tag="RH057_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HDV3128721.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(24169..25410)
                     /gene="agp"
                     /locus_tag="RH057_002365"
     CDS             complement(24169..25410)
                     /gene="agp"
                     /locus_tag="RH057_002365"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HDV3128722.1"
                     /translation="MNKTLIAAAVAGIVLIASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGMVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPVITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRTSAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(25699..>26149)
                     /locus_tag="RH057_002366"
     CDS             complement(25699..>26149)
                     /locus_tag="RH057_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128723.1"
                     /translation="GVNLQSDNKIQGQGGFLSWSFLSFKKEFIDSCLNDKKCILHLPE
                     FIFNDNKVRLVLDTDPEQRVNEVKNFLTALSDSICSLFIVNSNMASISLGNESFSTDE
                     DLEYGYLMNTGNHYDVYLPPELFAQAYKLNNKEMNAQLDYLNRYAIY"
BASE COUNT         6357 a         6788 c         6632 g         6372 t
ORIGIN      
        1 gtcacccaac ataccattaa tacttcgccg cctttccttc ggttaatggg cacgcctacg
       61 agcatcccga atgatacctg gttacagggg cattcgtttc tctcaaccgt agcaaaatat
      121 agtaatgatc ttcctcaggc tgaaatgaca gccagagagc ttgcttataa cgcaccagga
      181 aatcagggac tgcgcattga ttacgcgagt gtgttacaag cccgcggttg gcctcgtgca
      241 gcagaaaatg aattaaaaaa agcagaagtg atcgagccac gtaatattaa tctggaggtt
      301 gaacaagcct ggacagcatt aacgttacaa gaatggcagc aggcagttgt cttaacgcac
      361 gatgttgtcg aacgtgaacc gcaagatccc ggcgttgtac gattaaaacg tgcggttgat
      421 gtacataatc ttgcagagct tcgtatcgct ggctcaacag gaattgatgc cgaaggcccg
      481 gatagtggta aacatgatgt cgacttaacc accatcgttt attcaccacc gctgaaggat
      541 aactggcgcg gttttgctgg attcggttat gccgatggac aatttagcga aggaaaaggg
      601 attgttcgcg actggcttgc gggtgttgag tggcggtcac gtaatatctg gctcgaggca
      661 gagtacgctg aacgcgtttt caatcatgag cataaacccg gcgcgcgcct gtctggctgg
      721 tatgatttta atgataactg gcgtattggt tcgcaactgg aacgcctctc tcaccgcgtt
      781 ccattacggg caatgaaaaa tggtgttaca ggcaacagtg ctcaggctta tgttcgctgg
      841 tatcaaaatg agcggcgtaa gtacggtgtc tcctgggctt tcactgattt ttccgacagt
      901 aaccagcgtc atgaagtctc acttgagggt caggaacgca tctggtcttc accatatttg
      961 attgtcgatt tcctacccag tctgtattac gaacaaaata cagaacacga taccccatac
     1021 tacaacccta taaaaacgtt cgatattgtt ccggcatttg aggcaagcca tttgttatgg
     1081 cgaagctatg aaaatagctg ggagcaaata ttcagcgcag gtgttggtgc ctcctggcaa
     1141 aaacattatg gcacggatgt cgtcacccaa ctcggctacg ggcaacgcat tagctggaat
     1201 gacgtgattg atgctggcgc aacgctacgc tgggaaaaac gaccttatga cggtgacaga
     1261 gaacacaact tatacgttga attcgatatg acattcagat tttaaggata aatatgttac
     1321 gtaatggaaa taaatatctc ctgatgctgg tgagtataat tatgctcacc gcgtgcatta
     1381 gccagtcaag aacatcattt ataccgccac aggatcgcga atctttactc gccgagcaac
     1441 cgtggccgca taatggtttt gtagcgattt catggcataa cgttgaagac gaagctgccg
     1501 accagcgttt tatgtcagtg cggacatcag cactgcgtga acaatttgcc tggctgcgcg
     1561 agaacggtta tcaaccggtc agtattgctc aaattcgtga agcacatcga ggaggaaaac
     1621 cgctaccgga aaaagctgta gtgctgactt ttgatgacgg ctaccagagt ttttataccc
     1681 gcgtcttccc aattcttcag gccttccagt ggcctgctgt atgggccccc gtcggcagtt
     1741 gggtcgatac gccagcggat aaacaagtaa aatttggcga tgagttggtc gatcgagaat
     1801 attttgccac gtggcaacaa gtgcgagaag ttgcgcgttc ccggctcgtt gagctcgctt
     1861 ctcatacatg gaattctcac tacggtattc aggctaatgc caccggcagc ttattgcctg
     1921 tatatgtaaa tcgtgcatat tttactgacc acgcacggta tgaaaccgca gcagaatacc
     1981 gggaaagaat tcgtctggat gctgtaaaaa tgacggaata cctgcgtaca aaggttgagg
     2041 taaatccaca cgtttttgtt tggccttatg gcgaagcgaa tggcatagcg atagaggaat
     2101 taaaaaaact cggttatgac atgttcttca cccttgaatc aggtttggca aatgcgtcgc
     2161 aattggattc cattccgcgg gtattaatcg ccaataatcc ctcattaaaa gagtttgccc
     2221 agcaaattat taccgtacag gaaaaatcac cacaacggat aatgcatatc gatcttgatt
     2281 acgtttatga cgaaaacctc cagcaaatgg atcgcaatat tgatgtgcta attcagcggg
     2341 tgaaagatat gcaaatatca accgtgtatt tgcaggcatt tgctgatccc gatggtgatg
     2401 ggctggtcaa agaggtctgg tttccaaatc gtttgctacc aatgaaagca gatattttta
     2461 gtcgggttgc ctggcaatta cgtacccgct caggtgtaaa catctatgcg tggatgccgg
     2521 tattaagctg ggatttagat cccacattaa cgcgagtaaa atacttacca acaggggaga
     2581 aaaaagcaca aattcatcct gaacaatatc accgtctctc tcctttcgat gacagagtca
     2641 gagcacaagt tggcatgtta tatgaagatc ttgccggaca tgctgctttt gatggcatat
     2701 tgttccacga tgatgctttg ctttcagatt atgaagatgc cagtgcaccg gctatcacgg
     2761 cttatcagca agcaggcttt agcgggagtc tgagcgaaat tcgacaaaac ccggagcaat
     2821 ttaaacagtg ggcccgcttt aaaagtcgtg cgttaactga cttcacttta gaacttagtg
     2881 cgcgcgtaaa agccattcgc ggtccacata ttaaaactgc acgaaatatt tttgcacttc
     2941 cggtaataca acctgaaagt gaagcctggt ttgcacagaa ttatgctgat ttcctaaaaa
     3001 gctatgactg gaccgctatt atggctatgc cttatctgga aggtgtcgca gaaaaatcgg
     3061 ctgaccaatg gttaatacaa ttgaccaatc aaattaaaaa catccctcag gctaaagaca
     3121 aatctatttt agaattacag gcacaaaact ggcagaaaaa tggtcagcat caggctattt
     3181 cttcgcaaca actcgctcac tggatgagcc tattacaact gaatggagtg aaaaactatg
     3241 gttattatcc cgacaatttt ctgcataacc aacctgaaat agaccttatt cgtcctgagt
     3301 tttcaacagc ctggtatccg aaaaatgatt aatcgcatcg tatcgttttt tatattatgt
     3361 ctggtgttat gcatacccct atgcgtagcg tactttcact ctggtgaact gatgatgagg
     3421 ttcgttttct tctggccgtt ttttatgtcc attatgtgga ttgttggcgg cgtctatttc
     3481 tgggtctatc gtgaacgcca ctggccgtgg ggagaaaacg caccagctcc ccagttgaaa
     3541 gataatccgt ctatctccat tatcattccc tgttttaatg aggagaaaaa cgttgaggaa
     3601 accatacacg ccgctttagc acagcgttat gagaacattg aagttattgc cgtaaatgac
     3661 ggttcaacag ataaaacccg tgccatcctg gatcgcatgg ctgcacaaat tccccatttg
     3721 cgggtcattc atctggcgca aaaccagggg aaagccattg cgcttaaaac cggagctgcc
     3781 gcggcgaaaa gtgaatatct ggtgtgcatt gatggcgatg cgttattaga ccgcgatgcg
     3841 gcggcatata ttgtggaacc gatgttgtac aacccgcgtg tgggtgccgt aaccggtaat
     3901 cctcgtattc gaacacgttc taccctggtg ggtaaaattc aggttggcga gtattcctca
     3961 attattggtt tgatcaagcg aacccagcgt atctatggaa acgtatttac cgtttccggt
     4021 gttattgccg catttcgtcg cagcgccctg gcagaagtgg gttactggag tgacgatatg
     4081 atcaccgaag atattgatat tagctggaag ctgcagttga atcagtggac gattttttac
     4141 gagccacggg cactgtgctg gatattaatg cctgaaacgt taaaagggct gtggaaacag
     4201 cgcctgcgct gggctcaggg cggtgcagaa gtattcctca aaaatatgac aaggctatgg
     4261 cgcaaagaaa actttcgaat gtggccactg ttttttgaat actgtctgac gacaatatgg
     4321 gcctttacct gcctggtcgg tttcattatt tacgcagtcc aacttgccgg tgtaccgtta
     4381 aatattgaat tgacacatat cgctgcgaca catactgccg gaatattatt gtgtacgtta
     4441 tgtttactgc aatttattgt cagcctgatg atcgagaatc gctatgagca taatctgact
     4501 tcatcgcttt tctggattat ttggttcccg gttattttct ggatgctgag cctggcaacg
     4561 acattggtat catttacacg agtcatgttg atgcctaaaa agcaacgcgc ccgttgggta
     4621 agtcccgatc gcgggattct gagaggttaa tatgaacaat ttaattatta cgacccgaca
     4681 atcgccagta cgtttactgg ttgattatgt tgccacaacc atcttgtgga cattatttgc
     4741 tttgttcata ttcttattcg ccatggatct gctgacgggt tattactggc aaagcgaggc
     4801 cagaagccga cttcagttct attttttgct ggcagtggcg aatgccgtcg tgttaattgt
     4861 ctgggcgctg tacaataagc tgcgttttca aaaacagcag catcatgcag cctaccaata
     4921 tacgccgcaa gaatatgcag agagcttagc aatacctgat gagctctatc agcaactaca
     4981 aaaaagccat aggatgagcg tacacttcac cagccagggg caaataaaaa tggttgtttc
     5041 agaaaaagcg ctagtccggg cataaacacc caaaacaaag cccggttcgc ccgggctctg
     5101 caccgataat acacttaact gtatgcatgc agcgtacgtt ggcaaagtgc cgaacgtacg
     5161 cagtcctctt taccgaaccg gacgatccca accatttcat cttcttcgaa acgttccagc
     5221 gcgtcactta atccggagca cacgccgcga ggcaaatcgc attgcgtgat atcaccgttg
     5281 acgataaccg tcacgttctc cccgaggcgg gttaaaaaca ttttcatttg cgcggcagtc
     5341 acattctgcg cctcgtcaag aatgacgact gcattttcaa aggtacgtcc acgcatatag
     5401 gcgaacggcg caatttccac cttccctatt tccggtcgca ggcagtactg cataaaggaa
     5461 gcccctaagc gccggaccag cacgtcgtag accgggcgaa aatagggagc aaacttttct
     5521 gcgatatctc caggtaagaa gccaagatct tcatcggctt gcagaactgg acgggtgacg
     5581 ataatcctgt cgacatcctt atgtatcagg gcctctgccg cttttgctgc gctgatccag
     5641 gtttttccgc acccggcttc gcccgtggcg aatatcagct gcttactctc aatagccttc
     5701 agatagtgca attgcgcttc atttcgcgcg aggatgggcg aagtatcgcg actgtcgcgg
     5761 gccataccaa tggcttctac gccgcccatc tgcacaagcg aggtgaccga ttcttcttca
     5821 cgctgcttat ggctgcgcga atcccgtctc agcacacgtt ttgcctcacg acgagctttg
     5881 atcactgctt tttgtcttcc catggagagc accttgagtt gtttgtattc atcacacgcg
     5941 ccgttggcag cgcgattatg cgcacgaaca tcagagggtt ggcttccttg taagccatag
     6001 tttgcttttg gataaaatgc cgaaaaacgg ctacgcacac cgtttgcggc gtcggtaaca
     6061 cgagtaaaga gaggaagtga gaaaaataca gagtgacgaa gttgctaccg gaggagaaat
     6121 ctccgcaagt ggtggagcgt agcttaacaa gacattgtcc agaacaggac gttaccatcc
     6181 gcgatctcca tagtgactga ctatcactgc cgggaacttc cgctgctact taataattac
     6241 aacagatctc gcattcattg caacaatata ttcacttata tttaaccata aaacaccatt
     6301 tcagtgacat tagtttctac tggaaagatg acagagtgat gacagatatg aaaaaagctg
     6361 tgtgctttca gcaggattcg catcaggcat ctgcgcacga acgccggatg cggcgtgaac
     6421 gccttatccg cctaccgaac ccgcaccgat tgtaggcata agaagacgcg caagcatcgc
     6481 atcaggcatc tgcgcaagaa cgccttatcc gtctataaac attaaacccg caataacgcg
     6541 cttccgagat aatcgttcgc gtccttcact cccggcagcg caaaaaaata accgccgccg
     6601 ataggtttaa cgtattcctc cagcgcttcg ccattgagcc ttttttgtac agtcaggaag
     6661 cctttttcca gatcgtgttg gtaacagaca aacagtagcc ccatatccag ttgcccggag
     6721 ttggtgacgc ccagtgaata actgtagcca cgacgcagca tcaggctgga ctcactctcc
     6781 gccgtgcggg gattcgccag ccggatatgg ctgtccagcg caatcacctt cccttccgga
     6841 tcgctggcgt aatcgggaac atcatgctca tgctgcattc ccagcggcgc accggtttgc
     6901 ttatcacgac caaaaatcgt ctgctgttct ttcagcggag ttctgtccca aaattccact
     6961 cgaaactgaa tcaagcgtac tgcctgatag ctgccaccga ttgtccacgc tggctcctgc
     7021 tgatctgccg ttacccacac cactttttgc atcaacttat cattctggct atcgggattg
     7081 gcagtgccgt ctttgaaacc cagcaaatta atcggcgtct ctttgccttt actacgcgcc
     7141 gcgtgatcgg aaataaaccc ttcccgcttc cagcgcacgc tgagcaaatc tggcgtatgt
     7201 ttgatgatat cgcgcagcgc atggatcacc gtgtcctggg tgttggcgca aatttgtagc
     7261 aacacatcac catgacataa cgccgcatcc agcgagtcgt tgggaaaacg cgtcatcttc
     7321 tgcaactttt tcggcatctg tggcgcaagg ccaaagcgct catcaaacaa tgagtgaccc
     7381 accgataacg tgatggtgag attatcgggc gcaatgtagc cgccaagaat gccggaatcg
     7441 agtggtggca ggcgcggatt tggcgtttct ggtgccgctc cgccctgagt cagaaaagca
     7501 aaacgctgag tcaacaagcg aaacaaccgt tcaagatcgg ctttatcgct ggcaagcaca
     7561 tcaaacgcca ccagcatcat tgcggcctgt tgtggcgtca ggatccctgc ctgatgctta
     7621 ccataaaacg gctgtttctc attgcgtgca accggtgaaa gcgtacccgg cgcactttgc
     7681 gttttttgtg catgagcgac cggacaactt cccgccagcg ccagcgcacc aatgcctttc
     7741 agtaaacgtc ggcgtgacgg ttcattcacg ccgtttttat cttcatactg cataatgctt
     7801 aatccaatcc cagcacaccg cgaagttgcg ccagatcttc cgccagcgca gtaatcggtc
     7861 ctttcagtgc attccggtcg gcatcggtca atttgtcgta ggtttcaaaa ccgtctttag
     7921 tacggtattt cgccagaatg gtatcgacct ttttaaagtt ggcatcgact tttgccaaca
     7981 gttccgggtt agctttttgc agttgtggac gcagcagatc gacaattttc tgcgagcctt
     8041 caacgttagc ctggaaatcc cacagatcgg tgtggctgta gcgatcttct tcaccgctga
     8101 ttttgctggc tgccacttcc tcaatcagtc cggctgcacc gccgaccact tttgaaggtg
     8161 ggaaagccag ttcactgatg cgtttttgca aatcgaccac atcggtataa agctggtcag
     8221 cgtactgatc catgcctttg gtggtgttgt cgccaaacaa tgctttttcc agacggtgga
     8281 aaccggtgaa ttttggatca gcggcttttt gctcgtaatc atcttcacgg gcgtcaatgc
     8341 tgccatccag atcggagaac agttcagcaa tcggttcaat gcgctcatag tgctggcgcg
     8401 tcggcgcata cagtgctttc gctttttcga tatcgcctgc tttaatcgcg tcggtaaagg
     8461 ctttggtgtc ggtcaccagc tgcgtggttt ccgccatgac atacgcttta tatgcagtaa
     8521 ttgcaccacc aagacttaac agcgcatcac tttgcgccgc atccgccgtt gcctcacctt
     8581 tgacgatcaa cttccctttc gggttagtca gcagaccgca ggtcatatcg tattcgccag
     8641 gctgtaaatt cgccgtcatt ttctggctaa aaccaggggc gatattttcc cgctcttcca
     8701 ccaccatcac gcctttgagg atctcccact ccagcgcctt ctggctatgg ttctgaataa
     8761 tgaactgtgt tttcccggcg ttaaccgtaa tggtcatcgg ttcgcactgc ttatccgtca
     8821 cggtcacttt gacctgcgtc acatcagcgg cgttagccat aaaagcagaa gaaaacagcg
     8881 cagccacgct caactgcaat gcgttacggc ggaagttaat ggtcatgacg agactatccc
     8941 tttaaagtat gttgtaacta agcaagagtt tgcgtcgtat ttgttacgcg gagcgagacg
     9001 ctgtcgcccc tgcgcgtggt ggcagagcaa atgccaccag cgccgggatg agataaataa
     9061 accagacggc gacttcgctg acgctcggcg cttcctgata gccaaaaaac ccttccatca
     9121 gcgtgccaaa cagcgagtga gttgagagca ccgcactcat atcgaaggcg atttcctgaa
     9181 agtggttcca caatccggct tcatgaaatg cgcgaatggc accagctgcc agccctgcgg
     9241 cgacgaagag aataaacagg ctggtccatt taaaaaatgc accaagattg aggcgaatac
     9301 cgccccagta gagcaagaag cctagcacca cggcagtagc aagaccgagc attgcaccca
     9361 gcggcggcca gatcccgaca tcttgttgaa atgccgccag caggaaaaag accgactcca
     9421 gcccttccct tgcaacggca aaaaagacca tcatcaccag cgcccagcca tgatgatttc
     9481 cacgctgcaa tgcgctatcg actgcctgct ccagttgtac tttgacgttg cgcgacactt
     9541 tgcgcatcca gaaaaccatc caggtaagga tcaccacggc gatcaccgcc acgatacctt
     9601 caaacagttc ctgctctttt tgcggaaatt cgccggtggt ttcgttaatg aagatcccca
     9661 agcccaggca caacgcagcg gcaagcaaca cgccaatcca catcacacca atccatcggc
     9721 ctcgctgggt acgcttaaga tagctggcaa tcaaactgac aatcagcgcg gcttcaagtc
     9781 cttcgcgcaa cataatgaga aacggaacaa acatgccacc cacccttaaa cgtttttctg
     9841 agtcaatcta tgcaaagaaa agtaaatcgc agtgatagcg attatcatta cggtaaagaa
     9901 aaatacaagc ggaatgttta taaaatgatg ttgaaatgta aacaaagaga ggtaatgtca
     9961 ggggatataa acgattattc caggtacaaa gatgggattg tttacaacga gccatgtgtt
    10021 cttgccggat gccgcgtaaa cgccgtatcc agccgacaaa accgtcctga ctcaatacat
    10081 caaaacttcc atgtaaaact gataagcggg tgtatcaagc aatttcgtcc atcctcgatt
    10141 atcaaatcta actttttgcc cggaacataa gaacagcgcg ggtgttggtg gcgtcaaaat
    10201 cgctgaaacg ttccccgaag gccggggcag cagggggcgg cggcgattgg ccgccccctg
    10261 cgcgctccct gcaccattca atttatatgg cgaggcacat taagggagcg caacccgctg
    10321 ctgaagccaa acaaaaccgc cgcaggctaa gtcccttagc tttcctgcaa ccgtgacggc
    10381 ggagccgaat gatagtgcgc atcggcctcg gcaaagcgtt tttgcatcgc cgctgacggc
    10441 gctttaccca gcaaactaaa cactacaatc ccaatactgc cgaagataaa gcccggaata
    10501 atttcgtaca gtcccagcca gccgaactgt ttccagacga taaccgtcag cgccccgatg
    10561 atcatccccg ccagcgcacc gttgcgcgtc atgcgtgacc acatcaccga gaacagcacc
    10621 accggaccaa acgccgcgcc aaagcctgcc cacgcatagc tcactagccc cagcacacgg
    10681 ttttccgggt ttgccgccag cgcaatggcc accagcgcca ccaccagcac catcacacgc
    10741 cctacccaca ccagctcttt ctggctggca tgtttacgca gaaacgcttt gtataaatct
    10801 tcggtaatcg cactggagca caccagcagc tggcaactta aggttgacat taccgccgcc
    10861 agaattgccg acagcagaat cccggcaatc cacgggttaa acagaatttg cgccagttcg
    10921 ataaacacgc gctcagcatt ctggtttacc gcacctgcta ccgccgggtg ctcattaaag
    10981 taagcaatac caaagaagcc gacagctacc gcccctgcca ggcagaggat catccaggtc
    11041 atactgatac tacgcgcatg gacaatgctg tggtgagagt ctgccgccat aaaacgcgcc
    11101 aggatgtgcg gctgcccgaa gtaacccagc ccccaaccca tcagtgagat aatggcgaca
    11161 aagttcagcc ctttgagcat atcaacgttt tcgatgctct tttgtttgat cacttccagc
    11221 gagacaccaa agccaccgac actgataatg acgataaccg gcgtcaggat cagggcaaaa
    11281 atcatcaggc tggcctgtac agtgtcagtc cagctcaccg cgaggaaacc gccaataaag
    11341 gtgtaaagga tcgtcgccgc agctcccgcc cacagagccg tttcgtagct catgccaaag
    11401 gtactttcaa acagacgcgc gcctgccaca atgcccgaag cgcaataaat ggtgaagaac
    11461 agcaaaatca ccagcgcgga gataatgcgc aaaatgcggc ttttatcttc aaagcgcccg
    11521 gtgaaataat ccggcaatgt taaggcgtta ttgttgtatt cggtatgcac acgcaaccgc
    11581 cccgccacca gcttccagtt gatccacgcg cctaatgtca ggccaatggc gatccaactt
    11641 tcggaaatcc cggaaagaaa aaccgcgccc ggcaacccca ttaacagcca gccgctcata
    11701 tccgacgcac ccgccgataa tgccgtcacg aatggcccaa ggctacgacc gcccagaata
    11761 tagtcgtcaa agtttttcgt tgatcgccag gcgataaacc caatcaatat catgccaaag
    11821 atatagacac aaaatgtcac caacatcggt gtgctaatag ccatctaaag tctccaaaaa
    11881 attattatcg gcaatgtcga aacttgccgt tatatctgcc accggaacgg ggtaacagag
    11941 tttatgtttt accagggcga ccgtatcctg ccggaagcgc tggttattca caatccattt
    12001 aacacaccat ttacattaaa ttttagtgct cagcgacact atttttcatc aggttgcact
    12061 ctctcacatt ttttgcggtt gcacctttca aaaatgttaa ctgccgcaga gaaaaagtct
    12121 gagttatttt tttaatccct gtcatatcga tttcttttat taacatttca ttcattttta
    12181 agcttgctac gcatgtcaca tttaacatgg ttgcacaaag ttgcaacatc atggatattt
    12241 cacgataacg ttaagttgca cctttcagaa caacaggagt aatggcatgg gaaccaccac
    12301 catgggggtt aagctggacg acgcgacgcg tgagcgtatt aagtctgccg cgacacgtat
    12361 cgatcgcaca ccacactggt taattaagca ggcgattttt tcttatctcg aacaactgga
    12421 aaacagcgat actctgccgg agctacctgc gctgctttct ggcgctgcca atgagagcga
    12481 tgaagcaccg actccggcag aggaaccaca ccagccattc ctcgactttg ccgagcaaat
    12541 tttgccccag tcggtttccc gcgccgcgat cacagcggcc tatcgccgcc cggaaaccga
    12601 agcggtttct atgctgctgg aacaagcccg cctgccgcag ccagttgctg aacaggcgca
    12661 caaactggcg tatcaactgg ccgataaact gcgtaatcaa aaaaacgcca gtggtcgcgc
    12721 aggtatggtt caggggttat tgcaggagtt ttcgttgtca tcgcaggaag gcgtggcgct
    12781 gatgtgtctg gcggaagcgt tgttgcgtat tcccgacaaa gccacccgtg atgcgttaat
    12841 tcgcgacaaa atcagcaacg gtaactggca atcacacatt ggtcgtagcc cgtctttgtt
    12901 tgttaatgcc gccacctggg ggctgctgtt tactggcaaa ctggtttcca cccataacga
    12961 agccagcctc tcccgctcgc tgaaccgcat tatcggtaag agcggtgaac cgctgatccg
    13021 caaaggtgtg gatatggcga tgcgcctgat gggcgagcag ttcgtcacgg gtgaaaccat
    13081 tgcagaagcg ttagccaatg cccgcaagct ggaagagaaa ggtttccgtt actcttacga
    13141 tatgcttggc gaagctgcac tgactgccgc agatgcacag gcgtatatgg tttcctatca
    13201 gcaggcgatt cacgccattg gtaaagcgtc caatggtcgt ggcatctatg aagggccggg
    13261 catttcaatc aaactgtcgg cgctgcatcc gcgttatagc cgcgcccagt atgaccgggt
    13321 aatggaagag ctttacccgc gtctgaaatc actcaccctg ctggcgcgtc agtacgatat
    13381 tggtattaac atcgatgccg aagaggccga tcgtctggag atctccctcg atctgctgga
    13441 aaaactctgt ttcgagccgg aactggcagg ctggaacggt atcggttttg ttattcaggc
    13501 ttatcaaaaa cgctgcccac tggtgattga ttacctgatt gatctcgcca cccgcagccg
    13561 tcgccgtctg atgattcgcc tggtgaaagg cgcgtactgg gatagtgaaa tcaagcgtgc
    13621 gcagatggac ggccttgaag gctatccggt ttatacccgc aaggtgtata ccgacgtttc
    13681 atatctcgcc tgtgcgaaaa agctgctggc ggtgccgaat ctaatctacc cgcagttcgc
    13741 gacgcacaac gcccatacgc tggcagcgat ttatcaactg gcaggtcaga actactatcc
    13801 gggtcagtac gagttccagt gcctgcatgg tatgggcgag ccactgtatg agcaggtcac
    13861 cgggaaagtt gccgacggca aacttaaccg tccgtgtcgt atttatgctc cggtcggtac
    13921 ccatgaaacg ctgttggcgt atctggtgcg tcgcctgctg gaaaacggtg ctaacacctc
    13981 gtttgttaac cgtattgccg acacctcttt gccactggat gaactggtcg ccgatccggt
    14041 cactgctgta gaaaaactgg cgcaacagga agggcaaact ggattaccgc atccgaaaat
    14101 tcccctgccg cgcgatcttt acggtcacgg gcgcgacaac tcggcagggc tggatctcgc
    14161 taacgaacac cgtctggcct cgctctcctc tgccctgctc aatagtgcac tgcaaaaatg
    14221 gcaggccttg ccaatgctgg aacaaccggt agcggcaggt gagatgtcgc ccgttattaa
    14281 ccctgcggaa ccgaaagata ttgtgggctt tgtgcgtgaa gccacgccgc gtgaagtaga
    14341 acaggcgctg gaaagtgcgg ttaataacgc gccaatctgg tttgccactc ctccggctga
    14401 acgcgcggca attttacatc gcgctgccgt gctaatggaa agccagatgc agcaactgat
    14461 tggtattctg gtgcgtgaag ccggaaaaac cttcagtaac gccattgccg aagtgcgcga
    14521 agcggtcgat tttctccact actacgccgg acaggtgcgg gatgatttcg ctaacgaaac
    14581 ccaccgtcca ttagggcctg tggtgtgtat cagtccgtgg aacttcccgc tggctatttt
    14641 caccgggcag atcgctgccg cactggcggc aggtaacagc gtgctggcaa aaccggcaga
    14701 acaaacgccg ctgattgccg cgcaagggat cgccattttg ctggaagcgg gtgtaccccc
    14761 tggcgtggtg caattgctgc caggtcaggg ggaaaccgtg ggcgcgcaac tgacgggtga
    14821 tgatcgcgtg cgcggggtga tgtttaccgg ttcaaccgaa gtcgctacgt tactgcagcg
    14881 caatatcgcc agccgcctgg acgctcaggg tcgccctatt ccgctcatcg ctgaaaccgg
    14941 cggcatgaac gcgatgattg tcgattcttc agcactgacc gaacaagtag taatagatgt
    15001 gctggcctcg gcgttcgaca gtgcgggtca gcgttgttcg gcactgcgcg tgctgtgcct
    15061 gcaagatgag attgccgacc acacgttgaa aatgctgcgc ggcgcaatgg ccgaatgccg
    15121 gatgggtaat ccgggtcgcc tgaccaccga tatcggtccg gtgattgata gtgaagcgaa
    15181 agccaatatt gagcgccata ttcagaccat gcgtagcaaa ggccgtccag tgttccaggc
    15241 ggtgcgggaa aacagtgaag atgcccgtga atggcaaagc ggcacctttg tcgccccgac
    15301 gctgatcgaa ctggatgact ttgccgaatt gcaaaaagag gtctttggtc cggtgctgca
    15361 tgtggtgcgt tataaccgta atcagctgcc ggagctgatc gagcagatta acgcttccgg
    15421 ctatggtctg acgcttggcg tccatacacg tattgatgaa accatcgccc aggtcactgg
    15481 ctcggcccat gtcggtaacc tgtacgttaa ccgtaatatg gtgggcgcag tggttggtgt
    15541 gcagccgttc ggcggcgaag ggttgtccgg taccgggccg aaagcaggcg gtccgctcta
    15601 tctctaccgt ctgctggcga atcgcccgga aagtgcgctg gcagtaacgc tcgcgcgtca
    15661 ggatgcagag tatccggtcg atgcgcagtt gaaagccgca ttgactcagc cgctaaatgc
    15721 actgcgggaa tgggcagcaa atcgtccaga attgcaggcg ttatgtacgc aatatggcga
    15781 gctggcgcag gcaggaacac aacgattgct gccagggccg acgggtgaac gcaacacctg
    15841 gacgctgctg ccgcgtgagc gcgtgttgtg tattgccgat gatgagcagg atgcgctgac
    15901 tcagctcgcc gccgtgctgg cggtgggcag ccaggtactg tggccggatg acgcgctgca
    15961 tcgtcagcta gtaaaggcat tgccatcggc agtcagcgaa cgtattcaac tggcgaaagc
    16021 ggaaaatata accgctcaac cctttgatgc ggtgatcttc cacggtgatt cggatcagct
    16081 tcgcgcattg tgtgaagccg ttgctgcgcg ggatggcgca attgtttcgg tgcagggttt
    16141 tgcccgtggc gaaagcaata tccttctgga acggctgtat atcgaacgtt cgctgagtgt
    16201 gaataccgct gccgctggcg gtaacgccag tttaatgact ataggttaaa caggtagccg
    16261 gaggatgtta caacctcctc cggcatcttt aacgtggtcg aatcccctca ataataatcc
    16321 gctgcacgtt ttcaaccgtt tgattgaaaa atatctcatc gcgcaacgtc gcgcctgtca
    16381 ccgcctccac ctgaggggcg aaatcggcgt aatgttgagt ggaagcccaa atcatgaaaa
    16441 tcaaatgctg cggatcaatc ggcgcgagtt tgccgctttt gacccaaccg gcaatcagcg
    16501 ccgatttctc atcaattaat gccttcaaat cgcccgtcag ttcatccatt aacagcggcg
    16561 cgcctgccag catctccata cagaacaggc gcgaagcctg cggataatcg cgtgagactt
    16621 ccagcttcag acggatgtac tctttgatcg ccgccagcgg ggcgaaatct tcacgaaacg
    16681 cttttaacgg tgccagccag atatcgagaa tctgccgcag cacggcaata tacagcgcct
    16741 cttttgacgg gaagtaatac agcagattgg tttttgaaac acccgccaac tctgcgatct
    16801 gctccagcct tgtgccgtga aaaccgaatt gtgaaaaagt gtccagtgct gcgctaagaa
    16861 tcgctttttt cttcgcgctt actgcgcgcg aacgtttacc cgttgttttc actgcgcctt
    16921 gcgtcatgcg ctctcccctc ttgtagattg gcaacaggat agcaaaaccc ccgcccggcc
    16981 tcgaccttct gcactctcat cgcgcacaga tgcatgtttt atgtgcaact gttttgaccg
    17041 tttagtccac tttttaccag atattttaga caacacaatg ttaacacatt aataacattc
    17101 actttttaaa aactggcatc cgctttgcaa acaagccatt acgcggctga aaaggatatg
    17161 caggatgcag cgccccgtct gactttcact ttacgagacg aagagaggtt gatgatgaaa
    17221 attggcgtat tcgtacctat tggcaacaac ggctggctca tttcgaccca cgcgccgcag
    17281 tacatgccga cctttgaact gaataaagcc attgtgcaaa aagcggagca ctaccatttc
    17341 gatttcgccc tgtcgatgat caaactgcgt ggctttggcg gcaaaactga gttctgggat
    17401 cacaaccttg agtcgttcac cttgatggcg gggctggcgg ccgtgacctc gcgcattcag
    17461 atttacgcca ctgctgccac cttaacgtta cctccggcaa tcgtcgcccg tatggccgca
    17521 accatcgact ccatctctgg cgggcgtttt ggcgtcaacc tcgtgactgg ctggcaaaag
    17581 cccgagtatg agcagatggg tatctggcct ggcgatgact atttctcccg tcgttacgac
    17641 tatctcaccg aatatgttca ggtgctgcgc gacctgtggg gcacggggaa aagcgatttt
    17701 aaaggcgatt ttttcaccat gaatgattgt cgcgtcagtc cgcaaccgag tgtccccatg
    17761 aaagtgatct gcgccgggca aagcgacgct ggcatggcgt tctccgccca gtatgccgat
    17821 ttcaacttct gtttcggcaa aggcgtaaat acgcccacgg ctttcgcccc gaccgctgcg
    17881 cggatgaaac aggccgcaga gcaaaccggg cgcgacgttg gttcttatgt gttgtttatg
    17941 gtgattgccg atgaaaccga cgatgccgct cgcgccaaat gggaacacta caaagcgggc
    18001 gcggatgaag aggcgttaag ctggctaacc gaacaaagtc agaaagatac ccgctccggt
    18061 actgatacca acgtccgtca gatggccgat cccacttcgg cggtaaacat caatatgggg
    18121 acgttagtcg gttcttacgc cagtgtcgcg cgcatgttag atgaagtcgc aagcgtgcct
    18181 ggtgccgaag gcgtgctgtt gaccttcgat gattttctgt cgggaatcga aaccttcggc
    18241 gagcgcattc aaccactgat gcagtgccgc gcccatctac ctgcgctgac tcaggaggtg
    18301 gcatgatgac aaccttaacc gctcgaccgg aagccattac cttcgatccg cagcaaagtg
    18361 cgctgatcgt ggtggatatg caaaacgctt atgccacgcc aggcggctac ttagatctcg
    18421 ccgggtttga tgtctcaacc actcgcccgg tcattgccaa cattcaaacc gccgtgaccg
    18481 cagcgcgagc ggcagggatg ctgatcatct ggtttcaaaa tggctgggat gaacagtatg
    18541 tcgaagctgg cggccccggc tcaccgaatt ttcataaatc gaacgccctg aaaaccatgc
    18601 gtaagcagcc gcagctgcag gggaaattgc tggcgaaagg ctcctgggat tatcaactgg
    18661 tggatgaact ggtgccgcag cctggcgata ttgtgctgcc gaagccgcgc tacagcggtt
    18721 tcttcaatac accgctggac agcattttgc gcagccgtgg aatacgccat ctggttttca
    18781 ccggtatcgc taccaacgtc tgcgtcgaat cgacgctacg cgatggcttt tttctggagt
    18841 atttcggcgt ggtgctggaa gacgcaacac accaggcggg gccggaattt gcacagaaag
    18901 ccgcgttgtt caatatcgaa accttttttg gctgggtcag cgacgtcgaa acgttctgcg
    18961 acgcgctttc tcccacatcc tttgctcgta tcgcttaagg agtttaacga tgccaaaatc
    19021 cgtaattatt cccgctggca gcagcgcacc gctggccccc ttcgttcccg gcacgctggc
    19081 tgatggcgtg gtgtatgtct ccggtacgct ggcttttgat caacataata acgtgctgtt
    19141 tgccgatgac ccaaaggcgc aaacccgcca cgttctggaa actatccgca aggtgatcga
    19201 gacggcgggt ggcacgatgg cggatgtgac cttcaacagc atctttatta ccgactggaa
    19261 aaattacgcc gcgattaacg aaatctacgc cgagtttttt ccgggtgata aaccggcgcg
    19321 attctgcatt cagtgcggac tggtaaaacc tgacgcgctg gtggaaatcg ccacaattgc
    19381 gcatatcgcc aagtgaggcc gcgatgaaac tttcactctc acctccccct tatgctgatg
    19441 cgcccgtagt ggtgttgatt tcgggtcttg ggggtagcgg cagttactgg ttaccgcaac
    19501 tggcggtgct ggagcaggag tatcaggtag tctgttacga ccagcgcggc accggcaata
    19561 atcccgacac gctggcagaa gattacagta tcgcccagat ggcagcggaa ctgcatcagg
    19621 cgctggtagc cgcagggatt gagcattacg cagtggtcgg ccatgcgctc ggtgcgctgg
    19681 tgggaatgca gctggcgctg gattatcccg cgtcggtaac tgtgctgatc agcgttaacg
    19741 gctggctacg aataaacgcc catacgcgcc gctgttttca ggttcgcgaa cgattactgt
    19801 atagcggcgg cgcgcaggca tgggtggaag cgcagccgtt gttcctctat cccgccaact
    19861 ggatggcggc ccgcgcacct cgccttgagg cagaagacgc gctggcactg gcgcattttc
    19921 agggcaaaaa taatttactg cgtcgactta acgccctcaa acgcgctgac tttagtcacc
    19981 atgcggatcg catccgctgc ccggtgcaaa tcatctgcgc cagtgatgat ctgctggtgc
    20041 catcagcatg ttccagtgag cttcatgccg ccctgcccga tagccagaaa atggtgatgc
    20101 gctatggcgg acacgcctgc aacgtgaccg accccgaaac gtttaatgct cttttactca
    20161 acgggcttgc cagcctgtta catcaccgcg aagccgccct gtaaggaatt gctatgaacg
    20221 aagccgttag cccaggtgcg cttagcaccc tgttcaccga tgcccgcact cacaacggct
    20281 ggcgggagac acccgtcagc gatgagacgt tacgggagct ttatgccctg atgaaatggg
    20341 ggccgacatc agctaactgt tctccggcac ggatcgtgtt tatccgcact gtagaaggaa
    20401 aagaacgtct gcgcccggcg ctttccagcg gcaatctaca aaaaaccctg accgcgcccg
    20461 tcaccgctat cgttgcctgg gacagtgaat tttatgaacg gttaccacta ctgtttcccc
    20521 acggtgatgc ccgcagttgg tttacctcca gcccacaact tgccgaagaa acagcgtttc
    20581 gcaacagctc catgcaggcg gcctatctga tcgtcgcttg ccgtgcgctg ggactggata
    20641 ccggcccgat gtcgggtttt gaccgtcaat acgttgacga cgcttttttt gcaggcagca
    20701 cgctgaagag caatctgctg attaatatcg gctatggcga tagcagcaaa ctttatgcgc
    20761 gcctgccacg tctgtccttt gaagaagcct gcgggctgtt gtaaggagca aactatgaac
    20821 atagttgatc aacaaacctt tcgcgatgcg atgtcctgta tgggcgcggc ggtcaatatc
    20881 atcaccacgg acggtccagc cgggcgcgcc gggttcaccg ccagcgccgt ctgcagcgtg
    20941 accgatacgc cgcccacatt actggtgtgc ctgaatcgtg gggcgtccgt ctggccggta
    21001 ttcaatgaaa atcgaacgct gtgtgtaaat acactgagcg ccgggcagga gccgctttca
    21061 aatctttttg gcggcaaaac gcccatggaa caccgctttg ccgccgcccg ctggcagacg
    21121 ggtgtgaccg gatgcccgca actggaagag gctctggttt cgtttgattg ccgtatcagc
    21181 caggtggtga gcgtcggcac ccacgacatt ctgttttgcg ccatcgaagc gattcatcgt
    21241 cacgccacac cctacgggct ggtgtggttc gatcgcagtt atcacgcgct gatgcgccct
    21301 gcttgttaac ctcctaaagg agacagctga tggcaatgtt cggttttcct cactggcagt
    21361 taaaatcgac ctctacagag agcggcgtgg tcgcgccgta tgaacgattg ccgtttgctc
    21421 agacggcagt aatgggcgtt caacacgcgg tggcgatgtt tggcgcgacg gtgttaatgc
    21481 cgattctgat ggggctggat cccaatcttt ccattttaat gtcggggatc ggcacgttac
    21541 tgtttttctt tattaccggc gggcgcgtcc ccagttatct cggttccagc gcggcttttg
    21601 tcggcgtggt gattgccgcg acgggcttta acggtcaggg cattaacccg aatatcagca
    21661 ttgcgctggg cgggattatc gcctgtgggc tggtttatac cgtcattggc ctggtggtga
    21721 tgaaaattgg tacacgctgg attgaacgac taatgccgcc ggtggtgacg ggtgcggtgg
    21781 tgatggcgat tggcctgaac ttagcgccta tcgcagtgaa aagtgtatcg gcttcggcct
    21841 ttgatagctg gatggcggta atgacggtgc tgtgtattgg gctggtggcg gtgtttaccc
    21901 gtggcatgat ccagcggtta ttgattctcg taggattaat tgtcgcctgt ctgctgtatg
    21961 gcgtgatgac caacgtttta gggctgggaa aagccgtcga ttttactctc gtcagccatg
    22021 ctgcctggtt cggtttgcca catttttcca ctcctgcttt taatggtcag gcaatgatgc
    22081 tgattgcgcc cgtggcggtg attctggtgg cggaaaactt aggtcatctc aaggcagtcg
    22141 ccgggatgac cggacgcaat atggacccgt atatggggcg ggcattcgta ggcgatggtc
    22201 tggcaacgat gctttccggc tctgtcggcg gtagcggcgt gaccacctat gctgaaaata
    22261 tcggtgtgat ggccgtgacc aaagtctact cgacgctggt gtttgttgct gctgccgtca
    22321 tcgccatgct gttgggcttt tcgccgaaat ttggcgcgct gatccatacc attcctgcac
    22381 cggtgattgg tggtgcttca attgttgtgt tcgggctgat tgccgtcgca ggcgcaagga
    22441 tctgggtaca aaaccgtgtc gatttaagcc agaacggtaa tttgattatg gtcgcagtaa
    22501 ccttagtgct gggcgcgggt gattttgctc tcacgctggg cggttttacg ttgggaggga
    22561 tcggtacagc aacctttggc gcgattttac tcaatgcgtt gttaagcaaa aggttggttg
    22621 acgttccacc gccagaagtt gttcatcagg agccataaac agaaaaccgc cggggtaacc
    22681 ggcggtgatg actttttgac gagcttcaga caaaaatgca ttcgtcactg attagtccgg
    22741 ctagttgtca cttttaccgt gactgctctt gccacctttt ttacctgctt cagatgcgcg
    22801 ctgcgggtca ttcttgaaat tccccccgct gtgctgtcca ccttttttac ctgcttctga
    22861 tgctctttcg cggtcttctg caaaattgcc ggaaccgcct cgatggtttg ccattactga
    22921 cctccgtctg ggtatagaca tcatattgca tagaattaac gcggcgtatg gcggctgcca
    22981 tattttcgct acgtgggact aagcttagtg aactgacgcg ggcagacccg cgtttggtaa
    23041 tatcctgcaa cagcttatta gcgagaatag atatcatctc caataattat ccataagccg
    23101 ctcttatgat taaaaaaacg cgatgagata aaaatgtgtc attttgcgac aaaattacgt
    23161 gcttgtgaaa gttgttataa atcaaataag tggttgtgaa atttgcactc tgaaaaggac
    23221 gtcttatctt taaataagtg gtagcgaatc gctacggaat agagataaca cgaggagtgg
    23281 ttagaaatgg ctaaagttct ggtgctttat tattccatgt acggacatat tgaaacgatg
    23341 gcacgcgcag tcgctgaggg tgcaagcaaa gtcgatggcg cagaagttgt cgttaagcgc
    23401 gtaccggaaa ccatgccgcc gcaattattt gaaaaagcag gcggtaaaac gcaaactgca
    23461 ccggttgcaa ccccgcaaga actggccgat tacgacgcca ttatttttgg tacacctacc
    23521 cgctttggca acatgtccgg tcaaatgcgt accttcctcg accagacggg cggcctgtgg
    23581 gcttccggcg cactatacgg aaaactggcg agcgtcttta gttccaccgg tactggcggc
    23641 ggtcaggaac aaactattac ttcaacctgg acgacccttg cgcatcacgg catggtaatt
    23701 gtccccattg gctacgcagc gcaggaatta tttgacgttt cacaggttcg cggcggtacg
    23761 ccgtacggcg caaccaccat cgcaggcggt gacggctcac gccagccaag ccaggaagaa
    23821 ctgtctattg ctcgttatca aggggaatat gtcgcaggtc tggcagttaa acttaacggc
    23881 taatcttcaa caggaggata cgcatgccaa ctcaagaagc gaaagctcat cacgtcggtg
    23941 aatgggcatc tctgcgcaat acgtcgccgg aaatagccga ggccattttc gaagtcgccg
    24001 ggtacgacga aaaaatggcg gaaaagattt gggaagaagg tagcgatgaa gtgttagtca
    24061 aagcctttgc taagaccgat aaagattcgc ttttttgggg cgaacagacc atcgaacgta
    24121 aaaacgttta accagcgact cccccacttc tcgcggggga gttttctgtt atttcaccgc
    24181 ctcattcaac acgctatcaa acttatccat tgggcagaaa ccgttggcat ctatcgggca
    24241 accgcttaat tccagcgtca cgcgctgcgc aggtgcctgc agggttaacg catcggcatt
    24301 acgtaactgt tccgcactct ggtacacata ttcaattttc atcaaatcgc gattggcttt
    24361 gctgtcatgc caacgctgga aaacgatttt gccgccaatc ggcgtgcgtt cgttctggtc
    24421 atgcaactga tacggtttga aatccagcgc cgttaacaga gaggcaatgt tggagtcgtg
    24481 cccaaccaac actgtaattt tcggtgcgct ggtgcgatcg gtgaccagag ctttgtcgat
    24541 ataactgacc agcggtttcg caacattgcg cgccacttcc ggtgaggtaa acaggctgtc
    24601 ctggtagccg tttttaagct tcgacaacac cttccactgc tggtcagatt tgatttctcc
    24661 ccaggccacc tgatccatcg gaaaaccttc gtaatattgc aaagtaaacg catctaccag
    24721 cgagttgccg actttcagcg gcccggaaac accaggttct tgttgatact tggcgctaaa
    24781 ggtatttttg ccatccacca gcgaacactg ttgtttctct ttacaggcag gggaatcttt
    24841 atagttaacg attttttcca gtagttggta gctgtcggta agctggagtt tgctgagctc
    24901 tttctccatt gccgccaccg cctgttcact gaatgcggcg gaatcatcgg tgatcaccgg
    24961 gttaaaggtt gggtccatag ttcccatttt ttcctggtga tgcacaggaa tatcacaccc
    25021 cggaaatgcg ccggtaataa agaactgtgc ggtcgcgaca gtacgttgca gactattggc
    25081 ataggcataa acggtgtccg gcggcgggca ttcccccgat tttaccatcc cctgctctgc
    25141 cagccattca cgcatgtaat ggcccatata cacttcgagc acgccacctt tggtggtgag
    25201 ttgcccaccg gggacgtccc attctggcca tttattcggc gtcgactgct ccagcacact
    25261 gccattgttc gccagcggcg cacgtaagtt atggcggctc atcatgagca cttgctgtag
    25321 ctgatagcct tccggtacgg tttgtgcctg agcgtttgaa gcgattaaaa ctatccctgc
    25381 cacagctgcg gcgattagcg ttttgttcat tcctggcacc tcttttgtta tcaatcacta
    25441 aagagtgtga cagaaatatg acatttttcc gggaacaggc tcgcaaaaca gcttgttgag
    25501 cggctgtttt tatattcatc tttaaaaaca ggtaaggttt gatgcaagct atgtctgctt
    25561 atcccctgcc tcgcgtacac cgctgatgat tacacctgtg gctaaacagg atatcagcgg
    25621 cgttactaat acagaggcaa tagtaccaat aataagagaa gtgaggctaa taacaatggt
    25681 ttttagcaca tgcccttgtc agtaaattgc ataacgattt aaatagtcga gttgcgcatt
    25741 catttcctta ttgtttaact tgtaagcctg agcaaaaagt tcaggaggga ggtaaacgtc
    25801 ataatgattg ccagtgttca ttaaataacc atactcaaga tcttcatctg ttgaaaagga
    25861 ttcattcccc aaggagattg atgccatatt actattaaca ataaataatg agcaaatgct
    25921 atctgaaaga gcagtgagga aatttttcac ctcattaacc ctctgctctg ggtcggtgtc
    25981 taaaacaagg cgtaccttgt tatcattaaa tataaattcg ggtaaatgca gtatgcattt
    26041 tttgtcattc aagcaactat caataaattc ttttttaaaa gacaaaaaac tccatgataa
    26101 aaaaccaccc tgcccctgaa ttttgttgtc agattgtaga ttaacgccg
//
LOCUS       DAPHDW010000058        24994 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.058, whole genome shotgun sequence.
ACCESSION   DAPHDW010000058 DAPHDW010000000
VERSION     DAPHDW010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24994
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.058"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(102..185)
                     /gene="yohP"
                     /locus_tag="RH057_002367"
     CDS             complement(102..185)
                     /gene="yohP"
                     /locus_tag="RH057_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HDV3128724.1"
                     /translation="MKIILWAVLFIFLIGLLVVTGVFKMIF"
     gene            409..1845
                     /gene="mdtQ"
                     /locus_tag="RH057_002368"
     CDS             409..1845
                     /gene="mdtQ"
                     /locus_tag="RH057_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
                     /protein_id="HDV3128725.1"
                     /translation="MNRDSFYPAIACFPLLLMLAGCAPMHETRQALSQQTPAAQVDTA
                     LPTALKNGWPDSQWWLEYHDNQLTSLINNALQNAPDMQVAEQRIQLAEAQAKAIATQD
                     GPQIDFSADMERQKMSAEGLMGPFALNDPAAGTTGPWYTNGTFGLTAGWHLDIWGKNR
                     AEVTARLGTVKARAAEREQTRQLLAGSVARLYWEWQTQAALNTVLQQIEKEQNTIIAT
                     DRQLYQNGITSSVEGVETDINASKTRQQLNDVAGKMKIIEARLSALTNNQTKSLKLKP
                     VALPKVASQLPDELGYSLLARRADLQAAHWYVESSLSTIDAAKAAFYPDINLMAFLQQ
                     DALHLSDLFRHSAQQMGVTAGLTLPIFDSGRLNANLDIAKAESNLSIASYNKAVVEAV
                     NDVARAASQVQTLAEKNQHQAQIERDALRVVGLAQARFNAGIIAGSRVSEARIPALRE
                     RANGLLLQGQWLDASIQLTGALGGGYKR"
     gene            1898..2659
                     /locus_tag="RH057_002369"
     CDS             1898..2659
                     /locus_tag="RH057_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3128726.1"
                     /translation="MAQVAIITASDSGIGKECALLLAQQGFDIGITWHSDEEGAKDTA
                     REVVSHGVRAEIVQLDLGKLPEGAQALEKLIQRLGRIDVLVNNAGAMTKAPFLDMAFD
                     EWRKIFTVDVDGAFLCSQIAARQMVKQGQGGRIINITSVHEHTPLPDASAYTAAKHAL
                     GGLTKTMALELVRHKILVNAVAPGAIATPMNGMDDSDVKPDAEPSIPLRRFGATHEIA
                     SLVAWLCSEGANYTTGQSLIVDGGFMLANPQFNPE"
     gene            complement(2789..3367)
                     /locus_tag="RH057_002370"
     CDS             complement(2789..3367)
                     /locus_tag="RH057_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3128727.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDHQA"
     gene            3537..4124
                     /locus_tag="RH057_002371"
     CDS             3537..4124
                     /locus_tag="RH057_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HDV3128728.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLALTVILWGYGYRLF"
     gene            4298..5230
                     /gene="pbpG"
                     /locus_tag="RH057_002372"
     CDS             4298..5230
                     /gene="pbpG"
                     /locus_tag="RH057_002372"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HDV3128729.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(5268..6983)
                     /gene="dld"
                     /locus_tag="RH057_002373"
     CDS             complement(5268..6983)
                     /gene="dld"
                     /locus_tag="RH057_002373"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HDV3128730.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARINEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            7179..9476
                     /gene="bglX"
                     /locus_tag="RH057_002374"
     CDS             7179..9476
                     /gene="bglX"
                     /locus_tag="RH057_002374"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HDV3128731.1"
                     /translation="MKWLCSVGIAVSLALQPALADDLFGNHPLTPEARDAFVTELLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSTMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMEELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNAV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQSQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPMSFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            9687..10604
                     /gene="osmF"
                     /locus_tag="RH057_002375"
     CDS             9687..10604
                     /gene="osmF"
                     /locus_tag="RH057_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HDV3128732.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVVRGAITSGELDIYPEYTGNGAFFFKDENDAAWKNAQ
                     QGYEKVKKLDSEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPAFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWTK"
     gene            10611..11768
                     /gene="yehY"
                     /locus_tag="RH057_002376"
     CDS             10611..11768
                     /gene="yehY"
                     /locus_tag="RH057_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HDV3128733.1"
                     /translation="MTYLRINPVLALLLLLTVIAAALPFISYAPNRLVSGEGRHLWQL
                     WPQTIWMLVGVGCAWLTACFIPGKKGSICALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGSGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLIDALFDLLIALLKVKRND"
     gene            11761..12687
                     /gene="yehX"
                     /locus_tag="RH057_002377"
     CDS             11761..12687
                     /gene="yehX"
                     /locus_tag="RH057_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HDV3128734.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGEIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMTLLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVRLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVMNTQGQPCGTLHFQDLLEEA"
     gene            12692..13423
                     /gene="yehW"
                     /locus_tag="RH057_002378"
     CDS             12692..13423
                     /gene="yehW"
                     /locus_tag="RH057_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HDV3128735.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPVLQATLAGLGAIDASVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
     gene            complement(13404..13511)
                     /locus_tag="RH057_002379"
     CDS             complement(13404..13511)
                     /locus_tag="RH057_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohO"
                     /protein_id="HDV3128736.1"
                     /translation="MRIAKIGVIALFLFMAFGGIGGVMLAGYTFILRAG"
     gene            complement(13571..14302)
                     /gene="mlrA"
                     /locus_tag="RH057_002380"
     CDS             complement(13571..14302)
                     /gene="mlrA"
                     /locus_tag="RH057_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MlrA"
                     /protein_id="HDV3128737.1"
                     /translation="MALYTIGEVALLCDINPVTLRAWQRRYGLLKPQRTDGGHRLFND
                     ADIDRIREIKRWIDNGVQVSKVKMLLSNENVDVQNGWRDQQETLLTYLQSGNLHSLRT
                     WIKERGQDYPAQTLTTHLFIPLRRRLQCQQPTLQALLAILDGVLINYIAICLASARKK
                     QGKDALVVGWNIQDTTRLWLEGWIASQQGWRIDVLAHSLNQLRPELFEGRTLLVWCGE
                     NRTSAQQQQLTSWQEQGHDIFPLGI"
     gene            14524..16209
                     /gene="btsS"
                     /locus_tag="RH057_002381"
     CDS             14524..16209
                     /gene="btsS"
                     /locus_tag="RH057_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HDV3128738.1"
                     /translation="MYDFNLVLLLLQQMCVFLVIAWLMSKTPLFIPLMQVTVRLPHKF
                     LCYIVFSIFCIMGTWFGLHIDDSIANTRAIGAVMGGLLGGPVVGGLVGLTGGLHRYSM
                     GGMTALSCMISTIVEGLLGGLVHSILIRRGRTDKVFNPITAGAVTFVAEMVQMLIILA
                     IARPYEDAVRLVSNIAAPMMVTNTVGAALFMRILLDKRAMFEKYTSAFSATALKVAAS
                     TEGILRQGFNEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGKPISST
                     YTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLYEAKNR
                     LFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNALNTIK
                     AVIRRDSEQASQLVQYLSTFFRKNLKRPSEFVTLADEIEHVNAYLQIEKARFQSRLQV
                     NIAIPQELSQQQLPAFTLQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNA
                     GLYQPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
     gene            16206..16925
                     /gene="yehT"
                     /locus_tag="RH057_002382"
     CDS             16206..16925
                     /gene="yehT"
                     /locus_tag="RH057_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310963.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HDV3128739.1"
                     /translation="MIKVLIVDDEPLARENLRVFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            16972..17442
                     /locus_tag="RH057_002383"
     CDS             16972..17442
                     /locus_tag="RH057_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HDV3128740.1"
                     /translation="MLSNDILRSVRYILKANNNDLVRILALGNVEATAEQIAVWLRKE
                     DEEGFQRCPDIVLSSFLNGLIYEKRGKDESAPALEPERRINNNIVLKKLRIAFSLKTD
                     DILAILTEQQFRVSMPEITAMMRAPDHKNFRECGDQFLRYFLRGLAARQHVKKS"
     gene            complement(17483..17944)
                     /locus_tag="RH057_002384"
     CDS             complement(17483..17944)
                     /locus_tag="RH057_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HDV3128741.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKLTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
     gene            complement(18069..20069)
                     /locus_tag="RH057_002385"
     CDS             complement(18069..20069)
                     /locus_tag="RH057_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HDV3128742.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     GALIATFSDGVRTQLANSQALKEAQCSCGASGMCRHRVMLVLSYQRLCATVQPTEKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKGITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVEAKAQQAEFNHLIWQMRSEHVTSSDDPFASEEG
                     QTCRQYVQQLSQALWLGGISQPLIHYEAAFNRALQAAEACNWRWVSESLRQLRASVDA
                     FHTRASHYHAGECLRQLAALNSRLNCAQEMARRDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQENSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASACSPFAVERMAALLQQTDDPVSLVSGFVSFVDGQLTLEPRVMMTKTRAWALDAET
                     APVAPLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAISQAELLANDLSAV
                     GFYRLAHVLGQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
     gene            complement(20066..21202)
                     /locus_tag="RH057_002386"
     CDS             complement(20066..21202)
                     /locus_tag="RH057_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HDV3128743.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSGGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARQIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSLIPLARNFDFKSTLRANLQHWHPQHGKLYIESPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAVEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            complement(21195..23474)
                     /locus_tag="RH057_002387"
     CDS             complement(21195..23474)
                     /locus_tag="RH057_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128744.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFFSHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQAARRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQDESQALLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDFPGDALNRQREAFMARWIAWAVQQNNGDVLVVCGGWHAPALAKMWRECPQDIN
                     TPELPSLADAITGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHNLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFTPDGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEEHWKLTRPLSQHAAL
                     IEAACFGATLQEAARHKLEADMLDAGGIGSITTCLSQAALAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGISLSAAVALLERRSQAIHAPALDRGAAHGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPTFIAGFSSHLNQLS
                     DADFINALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQNWGVFHV"
     gene            complement(23485..24573)
                     /locus_tag="RH057_002388"
     CDS             complement(23485..24573)
                     /locus_tag="RH057_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HDV3128745.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATAISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
BASE COUNT         5849 a         6380 c         6809 g         5956 t
ORIGIN      
        1 tgcgccagtg attatatgaa gcaatgcaca tgacaggagc gcaacaggat gagagactga
       61 aatattctcg ttattttcgg acctgatgac attaattaat tttaaaatat catcttaaat
      121 acgccagtca ccaccagtag cccaatcagg aaaataaaca atacagccca gagtataatt
      181 ttcattggcg ttcctttatt tgtgagtgct gaataagtat aggaaaaaac gaagcccttt
      241 gccgttatcc ataaacgatt aagataaatc ggaagatatc attcacttag tgtatatttt
      301 tctggatatg tcgttttatt gctactgatg tattgccacg cgaaactaaa aaatatatcg
      361 caacacacgg taatctccgc gcaccttttc ttacctgatt attcccccat gaatcgtgat
      421 tccttttatc ccgccatcgc ctgttttccg ctgttactga tgctggccgg gtgtgcacct
      481 atgcatgaaa cccgccaggc gttaagccag caaacgcctg ctgcacaagt tgacaccgca
      541 ttacccacgg cgctgaaaaa tggttggcca gacagccaat ggtggctgga gtatcacgat
      601 aatcaactca cttccttaat taacaatgca ctacagaacg caccggatat gcaggtggct
      661 gaacagcgca ttcagctagc tgaagcccag gcgaaggcaa ttgctactca ggatggtccg
      721 cagatcgact tttctgcgga tatggagcgg caaaaaatgt cggcagaagg cttaatgggg
      781 ccgtttgctc tgaacgatcc ggccgcaggt acgaccggtc cgtggtacac caacggaact
      841 tttggcttaa cggcgggctg gcatctcgat atctggggaa agaatcgggc ggaggttact
      901 gcccgcctgg gtacggttaa agcacgggcg gcggaacgcg agcaaacccg ccaattgctg
      961 gctggcagcg tagcccgcct gtactgggag tggcaaaccc aggcggcgtt aaacacggtc
     1021 ttgcagcaaa tagaaaaaga gcagaacacc attatcgcga ccgatcgcca gctatatcag
     1081 aacgggatta cttcttcagt tgaaggtgtg gaaaccgata ttaatgccag caaaacccgg
     1141 cagcagctca acgatgtcgc ggggaaaatg aaaattattg aggcacggtt aagcgcactt
     1201 acaaataacc agacaaagtc attgaagctt aaaccggtcg cgttgccgaa agtggcaagc
     1261 cagcttcctg atgaactggg gtactcctta ctggcccggc gggcagattt gcaggcggcg
     1321 cactggtacg ttgagtcatc gctaagcacc attgatgcgg caaaagcggc attttatcct
     1381 gacatcaacc tgatggcctt cctgcaacag gatgcgttgc acttaagcga tctgttccgt
     1441 cattccgcgc agcaaatggg cgttacggca ggcctgacgc tacccatttt cgatagtggt
     1501 cgtcttaacg ccaatctcga tatcgcaaaa gccgaaagca acttgtctat cgccagctac
     1561 aacaaagcgg tggttgaagc ggtgaatgac gtggcgcggg cagccagtca ggttcagaca
     1621 cttgcggaga aaaaccagca tcaggcgcaa attgagcgcg atgccttgcg tgtggtaggt
     1681 cttgcgcagg cgcgctttaa cgcgggcatc attgctggtt cccgcgtcag cgaagccaga
     1741 atccccgcgc tgcgtgagcg ggccaatggc ctgttattgc aagggcagtg gctggatgct
     1801 tccattcaac tcactggtgc gttgggcggg ggttacaaac gctgatgata tgcgccttct
     1861 atacttaacg tttattcagc gttaagtgga gaactcgatg gcacaggttg cgattattac
     1921 cgcctccgat tcggggatcg gcaaagagtg cgcgttatta ctggcgcagc aggggtttga
     1981 tattggcatt acctggcact cagatgaaga aggggcaaaa gataccgcgc gtgaggtagt
     2041 tagccacggc gtacgtgcgg agatcgtgca gctggatctc ggtaaactgc cagaaggtgc
     2101 gcaggcgctg gagaaactca ttcaacggct ggggcgtatt gatgtgctgg tgaataatgc
     2161 gggcgcaatg accaaagcgc cgtttcttga tatggctttc gatgagtggc gcaagatttt
     2221 taccgttgat gtcgatggtg catttttatg ctcacaaatt gcggcccgcc agatggtgaa
     2281 acaagggcag ggcggtcgca tcatcaacat tacgtcggta catgaacata cgccgctgcc
     2341 ggatgccagc gcttacacag ccgctaaaca tgcgctcggc gggttaacca aaacgatggc
     2401 gctggagctg gtcaggcata agattttggt gaacgcagtc gcgcctgggg cgatcgccac
     2461 gccaatgaat ggcatggatg acagcgacgt gaagcccgac gcggagcctt cgattcctct
     2521 gcggcgtttt ggcgcaacgc atgagattgc cagcctggta gcgtggctgt gctcagaggg
     2581 cgcaaattac accaccgggc agtcgttgat agtggatggc ggctttatgt tggcgaatcc
     2641 gcagttcaac ccagaatagc gcagtgaggt gcgtggttat ttcaatgggt aaaattttgg
     2701 taggcatgat aagacgcaca agcgtcgcat caggcatggt gcaccggatg tcggatgcgg
     2761 cgcgaacgcc ttacccaggg caatggtttt acgcctgatg atccggcttt ttcttcccgc
     2821 gccgtttcag ccaccagcgc acgcccacca ccagaaccac gaccagaatc aaccagaccc
     2881 agtgcttcaa atgctggtcg agattgtgca accacggcgc aatcacctga ccaccagcgt
     2941 aaccaatagt ggtaaaaatc aacgcccagg caaatgcgcc gagaatattc agcggcagaa
     3001 agattttcgg cggcagctgg ctggcaccaa tcagcgtcgg gccaatcacc cgaaagccat
     3061 acataaagcg cgtaccaatg acaaacagat acggatggcg ttggataagt ttctgcgccc
     3121 gctcaatttt atcctgatgt ttcgagaaac ggcgtaacag cttgccgcca aaccgccgcc
     3181 cgcacagata gagcacctgg tcgccaatca tgccgccaag cgccacagaa agtaccacca
     3241 gcgggaactt taatagcccc tgatgcgccg caacgcctcc cagcaaagtc acggtttcac
     3301 cttccgccag gctaccgatc accagcgcgg cataaccata ttgtgagata agtgtattga
     3361 gatccatagg ccagtctgtc tccctttggt tgccagtaag catacaccct gtgaatcatt
     3421 cacgctgaaa aaggacgttc atcagacagt gaaaattaca tgggcgaaca aaaaataatc
     3481 acaagctgac ttatacttga actgtcaggt tcatgaactc acaacaaggg ggcggtatga
     3541 gccatgtctg gggactgttt tcccatcccg atcgtgaaat gcaggtgatt aatcgcgaaa
     3601 acgaaacgat ttctcaccat tacacccacc acgttttgct gatggcggcg attccggtga
     3661 tttgcgcctt cattggcact acacagattg gctggaattt tggcgatggc actatcctga
     3721 agttatcctg gtttaccgga ctggcgctgg ctgtcttgtt ttacggcgtt atgctggctg
     3781 gggtcgcggt gatggggcgg gtcatctggt ggatggcgcg taattatccg cagcgtccgt
     3841 cacttgcgca ctgcatggtc ttcgcgggct acgttgcaac tccgctgttt ttaagcggtc
     3901 tggtggcgct ttatccactg gtctggttgt gtgcgctggt cggcacggtg gcgctgtttt
     3961 acaccggcta cctgctttac cttggtattc cgagcttcct gaatatcaac aaagaggaag
     4021 gccttagctt ctctagttcg acgctggcga ttggcgtgct ggtactggaa gtgctgctcg
     4081 ccctaaccgt tatactatgg ggctacggtt atcgtttgtt ctagaaagtt ctttgacgtt
     4141 gcattgctgg cgtaaatgtc agcaatgcag catttcctca cgattctcct ttgacgatcg
     4201 cactttttgc tcgttatgat ggcgtcgcta gcctcagtaa atccttactc cggcggtgcg
     4261 caacccgtgc gcgtgaacca ctatctgaat gctcatcatg ccgaaatttc gagtttcttt
     4321 atttagcctg gccctgatgc tggctgtgcc ttttgcaccg caggccgttg cgaaaacggc
     4381 agccgctacc accgcttcac aaccggaaat tgcctccggt agcgcgatga ttgttgatct
     4441 gaataccaac aaagtgatct attcgaacca cccggatctg gtgcgtccga ttgcgtctat
     4501 cagcaaatta atgaccgcga tggttgtgct ggatgcacga ctgccgctgg atgaaaaact
     4561 aaaagtggat atcagccaga cgccggagat gaaaggggtc tattcgcgcg tacgactgaa
     4621 tagcgaaatc agccgtaaag atatgctgtt gctggcgctg atgtcttcag aaaaccgcgc
     4681 ggcggcaagc cttgcacacc attatcccgg tggttacaaa gcctttatta aggcaatgaa
     4741 tgcgaaagcg aaatcgctcg gaatgaacaa cacgcgcttt gttgaaccta ccggattgtc
     4801 ggtgcataac gtttcaactg cccgtgactt aaccaaactg ctcattgcca gcaaacaata
     4861 tccgttgatc gggcagttaa gtactacccg ggaagatatg gcaaccttct ctaacccgac
     4921 gtatacgctg ccgttccgca atactaatca tctggtgtat cgcgataact ggaatattca
     4981 gttaaccaaa accggcttta ccaatgcggc gggccattgt ctggtgatgc gtacggttat
     5041 caataataaa ccggtggcgc tggtagtgat ggacgcgttt ggcaaatata cccattttgc
     5101 cgatgccagc cgcctgcgta cctggattga aaccggtaaa gtgatgcctg tgccggcagc
     5161 agcgttgagc tataaaaaac aaaaagccgc ccaaatggcg gcggcggggc agacggcaca
     5221 gaacgattaa gtgaattcgg atggcgatac tctgccatcc gtaattttta ctccacttcc
     5281 tgccagtttt tccgtttact ggttttaccg atccccggat tcatgctgtt ggtcggatcg
     5341 ttctcgcgat agaacttctg caacgtctcc ggtgctttat acaaatgacc gacgttatgc
     5401 tcggcagggt attgcgcgcc gcgctgctgt agcagttcca gcatctgttc ttttaacgca
     5461 tgcacatcca cgcctttttt cactatgtaa tcctgatgga agacatagca cataaaatgg
     5521 ccgtaataga gcttgtgcac cagctggctg tcgatctccg gcggtaaatg ctcataccac
     5581 tcggtgtcgt tacgccgcag agcgatatcc aacgccagaa tgtcttcgac ttcatcggaa
     5641 tgcaccgcct gataacgaat tgctgcgccc gcagcggcga aacggtgtaa aaacgctttg
     5701 ctgccttcct ccggcgtaca gacaaagaaa tcgccttcgg cctgtttgaa ataatccacc
     5761 aaccacgatt tggcttcgcc cacgccatcg cccgccattt ttaacagcag atgatgctcg
     5821 tatttatcgc gccagttttt catgcgcggc ggtaaatggc tggggaacag gtgaccgaat
     5881 ttttgcatcg cacggtcagt aaaatgcgga cggaagaatt tcactttctc cagcatcgca
     5941 tcggtgcgtc ccttgagatt aaagaagaac ggcatcttgt cggtgcctaa cttatcaatc
     6001 atcaggaagg tgtctttgcc gtatttttcc gcaatatcgt agatatcccg gtgcatatat
     6061 tccccggcaa ccggcagatt ttcgaagtta gccagaatat gacggcggat ttcggtcagc
     6121 acttccggct ggttggtgcc gatataaaac acctgctgat ttttttccgc ttcgaaggta
     6181 tctagacgta ccgcaaagac ggccagcttc ccggcgcaac cagaagattc aaataaccga
     6241 tccggatcgg cgttataacg tgcgggcgtg tcggcttcaa tatcacgaac gcggtggaca
     6301 tagtcataat cgtgggcgtg acgaccatcg tgacgcacat cgtcatcttt gatgcgatca
     6361 tcatccagct tgctaaggat ctgctccggc gtttcgccca gatcaatccc cagatggttc
     6421 accagcgtca gtttgccgtc ttcatttata cgcgcgaata acgacatttc ggtatacgcc
     6481 gggccacgtt gcaccagcga gccgccggag ttgttacaaa taccgccgat aaccgatgcg
     6541 cctatacacg acgatccaat caccgagtgc ggttcgcgtc ccagcggttt gagggctttt
     6601 tccagcgaat agagcgtggt gcccggatag gccagcacct gttcgccctt gccaagaacg
     6661 tgcagcttgt cgagacgcag ggtgctgatg atcacgatat cgcgatcata gtcgttaccg
     6721 tttggcgtcg atccttcggt caggcctgta ttggcagcct gcatcagaat aattttgtcg
     6781 gcggtgacac aggctttcag cacccgccac aattccagta gtgagccagg gaaaacgaca
     6841 gccagcgcgt cgccctgacc agaacggaag cccttgcgat agcgggccgt ttttgcggga
     6901 tcggtgagca ggtgtgaatg accgaccaga cgggcaagtt cattcaaaaa ggctttatta
     6961 tcagttgttg tcatggaaga cattttccac tccttgtggt ggcgaaaaaa tatcacaaac
     7021 tagaatagcg ccttgtatga caattcagcg agtgaatatt ttgaaaattc agacaataaa
     7081 ctgagcattc tttttctctc tccatcatgc ttatggcaaa ctacggtttt tgcaccattt
     7141 tcgctgcctc gtcgggcaac agaggaagaa aaatccatat gaaatggcta tgttcagtag
     7201 gaatcgcggt gagtctggcc ctgcagccag cactggcgga tgatttattc ggcaaccatc
     7261 cattaacgcc cgaagcgcgg gatgcgttcg tcaccgaact gcttaagaaa atgacagttg
     7321 atgagaaaat tggtcagctg cgcttaatca gcgtcggccc ggataacccg aaagaggcga
     7381 tccgcgagat gatcaaagac ggtcaggttg gggcgatttt caacaccgta acccgtcagg
     7441 atatccgcgc catgcaggat caggtgatgg aattaagccg cctgaaaatt cctcttttct
     7501 ttgcttacga cgtgctgcac ggtcagcgca cggtgttccc gattagcctc ggtctggcct
     7561 cgtcttttaa cctcgatgca gtgaaaacgg tcggacgtgt ctctgcttat gaagcggcag
     7621 atgatggcct gaatatgacc tgggcaccga tggtcgatgt ctcgcgcgat ccgcgctggg
     7681 gacgtgcttc cgaaggtttt ggcgaagata cgtatctcac ctcaacaatg ggtaaaacca
     7741 tggtggaagc gatgcagggt aaaagcccgg cagatcgcta ctcggtgatg accagcgtca
     7801 aacactttgc cgcatacggc gcggtagaag gcggtaaaga gtacaacacc gtcgatatga
     7861 gtccgcagcg cctgtttaat gattatatgc cgccgtacaa agcggggctg gacgcaggca
     7921 gcggcgcggt gatggtggcg ctgaactcgc tgaacggcac gccagccacc tccgattcct
     7981 ggctgctgaa agatgttctg cgcgaccagt ggggctttaa aggcatcacc gtttccgatc
     8041 acggtgcaat caaagagctg attaaacatg gcacggcggc agacccggaa gatgcggtgc
     8101 gcgtggcgct gaaatccgga atcaacatga gtatgagcga cgagtattac tcgaagtatc
     8161 tgcctgggtt gatcaaatcc ggcaaagtga cgatggaaga gctggacgat gctgcccgcc
     8221 atgtactgaa cgttaaatat gatatgggat tgtttaacga cccatacagc catttggggc
     8281 cgaaagagtc tgacccggtg gataccaatg ccgaaagccg cctgcaccgt aaagaagcgc
     8341 gtgaagtggc gcgcgaaagc ctggtgttgc tgaaaaaccg tctcgaaacg ttaccgctga
     8401 aaaaatcggc caccattgcg gtggttgggc cactggcgga cagtaaacgt gacgtgatgg
     8461 gcagctggtc cgcagccggt gttgccgatc aatccgtgac cgtactgacc gggatcaaaa
     8521 atgccgtcgg tgaaaacggt aaagtgctgt atgccaaagg ggcgaacgtt accagtgaca
     8581 aaggcattat cgatttcctg aatcagtatg aagaagcggt caaagtcgat ccgcgctcgc
     8641 cgcaagagat gattgatgaa gcggtgcaga cggcgaaaca atctgatgtg gtggtggctg
     8701 tagtcggtga agcacagggg atggcgcacg aagcctccag ccggaccgat atcactattc
     8761 cgcaaagcca acgtgacttg attgcggcgc tgaaagccac cggtaaaccg ctggtgctgg
     8821 tgctgatgaa cgggcgtccg ctggcgctgg tgaaagaaga tcagcaggct gatgcgattc
     8881 tggaaacctg gtttgcgggg actgaaggcg gtaatgcaat tgccgatgta ttgtttggcg
     8941 attacaaccc gtccggcaag ctgccaatgt ccttcccgcg ttctgtcggg cagatcccgg
     9001 tgtactacag ccatctgaat accggtcgcc cgtataatgc cgacaagccg aacaaataca
     9061 cttcgcgtta ttttgatgaa gctaacgggg cgttgtatcc gttcggctat gggctgagct
     9121 acaccacttt caccgtctct gatgtgaaac tttctgcgcc gaccatgaag cgtgacggca
     9181 aagtgactgc cagcgtgcag gtgacgaaca ccggtaagcg cgagggggcc acggtagtgc
     9241 agatgtactt gcaggatgtg acggcttcca tgagtcgccc tgtgaaacag ctgaaaggct
     9301 ttgagaaaat caccctgaaa ccgggcgaaa ctcagactgt cagcttcccg atcgatattg
     9361 aggcgctgaa gttctggaat caacagatga aatatgacgc cgagcctggc aagttcaatg
     9421 tatttatcgg cactgattcc gcacgcgtta agaaaggcga gtttgagttg ctgtaatgct
     9481 ttagtaaggg cgcgacgttt gcgccctttg taggccggat aaggcgctcg cgccgcatcc
     9541 ggcaggaaac gccggaccga atatgcctct taagtattta tctgattctg caactgaatc
     9601 tttccgctca agctaacccc gccattatca actatgcttt tctcttaatt cgctgaaaac
     9661 aacagtaaaa gaatgaggaa agcatcatgc cactcttaaa gctctgggca ggttcactgg
     9721 ttatgttggc agccgtgagc ctgccgctac aggcggcttc ccccgttaaa gtcggttcaa
     9781 aaatcgatac cgaaggtgcg ctactcggca atatcatttt gcaggtgctg gaaagccacg
     9841 gcgtaccgac ggtgaataaa gtgcaactcg gaacgactcc tgtggtgcgc ggggcgatta
     9901 cttccggtga actggatatc tatccggaat ataccggcaa tggcgctttc ttctttaaag
     9961 atgaaaacga tgcagcgtgg aaaaacgcgc agcaaggtta cgagaaagtc aaaaagctcg
    10021 attcggagca caacaagtta atctggctga cgcccgcgcc tgcaaataac acctggacta
    10081 tcgccgtgcg tcaggatgtg gcagagaaaa ataaactcac ttcgcttgcc gacctgagtc
    10141 gttatctgca agagggcggc accttcaaac tggcagcctc ggcagagttt atcgaacgcg
    10201 ccgatgcgtt acccgcgttt gaaaaagcct atggctttaa gctcggtcag gatcagttgc
    10261 tgtcactggc tggtggcgac acggcggtga cgatcaaagc cgctgcccag caaacctctg
    10321 gcgttaatgc tgcaatggct tacggcactg acggcccggt agcggcgctg gggctgcaaa
    10381 ccttaagcga tccgcaaggc gtgcaaccta tctatgcgcc tgcaccagtg gtgcgtgaat
    10441 cggtgttgaa ggagtatccg caaatggcac agtggctaca gccagtcttc gccagcctcg
    10501 atgcaaaaac attgcagcaa ctgaatgcca gcattgcagt tgaaggactg gatgccaaaa
    10561 aagtggctgc cgactacctg aaacaaaaag ggtggacgaa gtaatttccc gtgacttatc
    10621 tacgtattaa tcctgttctg gcgctgctgc tgttgctgac ggtaatcgca gcggcgctgc
    10681 cgtttatcag ttacgcgcct aatcgtttag tttcgggtga ggggcgtcat ctctggcagc
    10741 tgtggccgca aacgatctgg atgctggtgg gcgttggttg cgcctggctg acggcctgtt
    10801 ttattcccgg taaaaaaggc agcatttgtg cactcattct ggcgcaattc gtcttcgtat
    10861 tgctggtgtg gggcgcggga aaggcggcga cacaactggc gcaaaatggc agtgcgctgg
    10921 cgcgtaccag cctcggcagt ggtttctggc tggctgcggc gctggcattg ctggcctgta
    10981 gcgatgccat ccgccgaatc tccacgcatc cgctgtggcg ctggttgttg catatgcaga
    11041 ttgccattat tccgctgtgg ttgctgtact ccggcacgct taacgatctc tcactaatga
    11101 aagaatacgc caaccgtcag gatgtgtttg acgacgcgct ggcacaacat ctgacgttgc
    11161 tgtttggtgc ggtgctgcct gcgttagtga ttggtgtgcc gttgggcatc tggtgctact
    11221 tttccactgc tcggcagggg gcaatttttt ctctgctcaa tgtcattcag accgtgcctt
    11281 cggtggcgct ctttggcctg ttgattgcgc cgcttgccgc gctggtgacg gcctttccgt
    11341 ggctggggaa gctcggcata gcaggaaccg gaatgacacc cgcactgatt gcgctggtgc
    11401 tctatgcctt gctgccgctg gtgcgcggcg tggtagtcgg tttgaaccag atcccgcgcg
    11461 atgtgctgga gagcgccaga gcgatgggca tgagcggggc gcagcgattc ctgcatgttc
    11521 agttaccact ggcgttaccg gtatttctgc gcagcctgcg ggtggtgatg gtgcaaactg
    11581 taggtatggc ggtgattgcg gcgttaatcg gcgcaggcgg ttttggtgcg ctggttttcc
    11641 aggggctgct aagcagcgcc attgatttag tgttgctggg ggtgatcccg gtaattgttc
    11701 tggcggtgct gatcgatgcc ctgttcgatt tgcttatcgc actgctgaag gtgaaacgta
    11761 atgattgaat ttagccatgt cagcaaactg tttggcgcac aaaaagccgt taacgatctt
    11821 aatctcaatt ttcaggaagg gagtttttcg gtgctgattg gcacatctgg ctccggcaaa
    11881 tccaccacct tgaaaatgat taaccgcctg gtggaacatg acagcggaga gatccgcttt
    11941 gccggagaag aaattcgctc gctgccagta ctggagttgc gccgccggat gggctatgcc
    12001 attcaatcta ttggcctatt cccccactgg agcgtggcgc aaaacatcgc taccgtgccg
    12061 caattacaaa aatggtcgcg ggcacggatt gacgatcgta tcgacgaatt aatgacgcta
    12121 ctggggctgg agtcaaattt gcgtgagcgt tatccgcatc agctttccgg tggtcagcag
    12181 caacgtgtgg gagtggcgcg cgcactggct gccgatccgc aagtcttact gatggatgaa
    12241 ccttttggcg cactggaccc ggtaacgcgc ggcgcgttgc aacaagagat gacgcgcatt
    12301 caccgtttgc tggggcgcac tattgtgctg gtcactcatg atattgatga ggcgctaagg
    12361 ctggcagaac atctggtatt gatggatcac ggtgaagtgg tgcagcaggg gaatccgctg
    12421 acgatgctga ctcgtccggc gaatgatttt gtccgccagt tttttggacg tagtgaactg
    12481 ggggtgcgcc tgctttcgtt acgtagtgtg gcggattacg tgcgtcgcga agaacgggca
    12541 gaaggtgagg cactggcaga agagatgacg ctacgcgatg cgctctccct gtttgtcgcg
    12601 cggggatgcg aggtgctgcc ggtgatgaac acgcagggcc agccttgcgg cacgctgcat
    12661 tttcaggatc tgctggagga ggcgtaagcg tatgaaaatg ttgcgcgatc cgctgttctg
    12721 gctcattgct ttgtttgtgg cactgatttt ctggctgcct tacagccagc cgctgtttgc
    12781 tgccttgttc ccacaactgc cacgacccgt ttatcagcaa gaaagttttg cagctctggc
    12841 actggctcat ttctggctgg tgggaatttc gagtttgttt gcggtgatca ttggcactgg
    12901 tgccggaatt gctgtcactc gcccgtgggg cgcggaattt cgcccactgg tggaaactat
    12961 tgccgccgtt ggacagactt ttccgcctgt cgcagtgctg gcgattgccg ttccggtgat
    13021 cggctttggt ctgcaaccag cgattatcgc cttgatcctt tacggtgtgc tgcccgtcct
    13081 gcaggcgaca cttgccgggc tgggagcgat cgatgccagc gtgacagaag ttgcgaaagg
    13141 tatgggaatg agtcgtggtc agcgactgcg taaggtcgag ctaccgctgg cggctccggt
    13201 gattctggcg ggcgtgcgaa cttcggtgat tatcaacatt ggtacggcga cgatcgcctc
    13261 aacggtaggg gccagcacgc tgggtacgcc catcatcatc gggcttagcg gatttaatac
    13321 cgcgtatgtg atccaggggg cgttactggt ggcactggcg gcgatcatcg cagaccgcct
    13381 gtttgaaagg ctggtgcagg cgcttagcca gcacgcaaaa taaaggtata acctgcgagc
    13441 atgacgccac caattccgcc gaacgccata aacaggaaca gggcgatgac cccaatttta
    13501 gctatgcgca taatgcactc cttatgttaa cgaaaggatt gtacagtaaa gcgcatttgt
    13561 taacgaatca ttaaatgccg agtgggaaaa tatcatggcc ttgttcttgc caactggtga
    13621 gttgctgctg ttgggcggag gttcgatttt caccgcacca caccagcaat gtacgacctt
    13681 cgaatagttc agggcgtagt tgattgagcg agtgggcgag gacatcaatg cgccatcctt
    13741 gttgactggc aatccagccc tccagccaca gacgggtggt atcctgaata ttccagccaa
    13801 ccaccagcgc atctttaccc tgttttttac gtgccgaagc cagacaaatg gcgatgtagt
    13861 tgatcagtac gccgtcgagg atcgccagca gcgcctggag agtcggttgt tggcactgaa
    13921 gccgtcggcg cagaggaata aacagatgtg tggtgagtgt ctgggcgggg taatcctgac
    13981 cgcgctcttt gatccacgtt cgcaggctat gcagattgcc gctttgcagg taggtcagta
    14041 atgtttcttg ctgatcgcgc cagccgttct gcacatcaac attttcatta ctgagcagca
    14101 ttttaacttt gctgacctgc acgccgttgt cgatccagcg tttgatctcg cggatacggt
    14161 caatatcggc atcgttgaat agtcgatgac cgccgtctgt ccgttgcggt ttcagcaacc
    14221 cgtaacgcct ctgccacgcg cgtaacgtga cagggttaat atcacaaagc aacgccactt
    14281 caccaattgt gtaaagcgcc atcgtctcac ccttgctcgc gaggtcccgg tttaacttta
    14341 gacgcagttt tgcgaaccag gtagttttgc ccgttttttg tgcatctata gggtgatttt
    14401 atttttgcca ggcgattttg agtgatcgta ctcacgaatt ctcatttttc tgcaagagtt
    14461 caaagaaagt taaacgcagg caatgtatgt tacgcgtttt aaagggaagt gtggtttgcg
    14521 ggtatgtacg attttaatct ggtgttgctg ctgcttcagc agatgtgcgt ttttttagtc
    14581 attgcgtggt taatgagtaa aacgccatta ttcataccgt taatgcaggt cacggttcgt
    14641 ctgccgcata aatttctctg ctacatcgtc ttttccatct tctgcatcat gggcacctgg
    14701 tttgggttgc acattgacga ttctattgcc aatacccgtg cgataggcgc ggtaatgggc
    14761 ggcttactcg gcggtccggt cgtcggtggg ctggttggtc tgaccggcgg cttacatcga
    14821 tattcgatgg ggggcatgac cgcgctaagt tgcatgatct caaccatcgt tgaaggattg
    14881 ctcggcggcc tggtacacag catcctgatc cgtcgcgggc gcactgataa agtctttaac
    14941 cccattaccg ccggtgccgt cacgttcgtc gctgaaatgg tgcaaatgct gatcatcctt
    15001 gcgatcgccc gaccttatga agatgcggtg cgtctggtga gtaatattgc tgcaccaatg
    15061 atggtcacca ataccgtcgg cgcggcgctg tttatgcgta tattgctcga taaacgcgcg
    15121 atgtttgaaa aatacacttc tgctttttct gccactgcgc tgaaagtggc agcctcgacg
    15181 gaaggcattt tgcgacaggg gtttaacgaa gtgaacagca tgaaagtggc tcaggtgctg
    15241 tatcaggagc tggatattgg tgcagtcgcg attaccgatc gagagaaatt gctggccttt
    15301 accggaattg gtgacgacca ccatttaccc ggcaaaccga tttcttcgac ttacacctta
    15361 aaagcgattg aaaccggtga agtggtctac gctgatggca acgaagtacc ttatcgttgc
    15421 tctttgcatc cgcaatgcaa actggggtcg acgctggtaa ttccgttgcg tggcgaaaat
    15481 cagcgcgtga tgggcaccat caaattgtat gaagccaaaa accgtttatt cagttcaata
    15541 aaccgcacgc tgggcgaggg gattgcgcaa ctgctttcgg cgcagattct tgccgggcaa
    15601 tatgagcggc aaaaagcgat gctcacccag tcagaaatca aactgcttca cgcccaggtg
    15661 aatcctcatt ttttgtttaa tgcgcttaac accattaaag cggtgatccg ccgcgacagc
    15721 gaacaggcca gccagctggt gcagtatctt tccacttttt tccgcaaaaa cttaaagcgg
    15781 ccttcggagt ttgttactct cgccgacgaa attgaacatg tgaacgctta tctgcaaatt
    15841 gaaaaggcgc gcttccagtc gcggttgcag gtcaacattg ctattccgca agaattatcc
    15901 cagcagcaat tgcccgcgtt taccctgcaa ccgatagtgg aaaacgccat taaacatggg
    15961 acatcacaac tgctggatac agggcgagtg gcaatcagcg cccgacgtga ggggcaacat
    16021 ttgatgctgg agatcgaaga caatgccggt ttgtatcaac cggtaaccaa tgccagtggg
    16081 ctggggatga atctggtgga taagcgttta cgtgaacggt ttggcgatga ctatggaata
    16141 agcgtcgcct gtgagcctga tagttacacc cgaataacgt tacgactacc atggagggac
    16201 gaggcatgat taaagtctta attgtcgatg atgaaccgtt agcacgggag aacctgcgcg
    16261 tatttttgca ggagcagagc gatattgaaa tcgttggaga gtgttcaaac gccgtggaag
    16321 ggatcggcgc ggtgcataaa ctgcgcccgg atgtgctgtt tctcgatatc cagatgccgc
    16381 gcatcagtgg tctggaaatg gtggggatgc tcgatccgga acatcgtccg tatattgttt
    16441 ttctcaccgc gtttgacgaa tacgctatta aagcctttga agaacatgct tttgattatc
    16501 tgctgaagcc aattgatgaa gcgcgactgg agaaaacgct ggcgcgattg cgtcaggagc
    16561 gcagcaagca ggatgtttcg ctgttaccgg aaaatcaaca ggcgctgaaa tttatccctt
    16621 gtacggggca tagtcggatt tatttgctgc aaatgaaaga tgtggcattt gtcagcagtc
    16681 ggatgagcgg tgtctacgtt accagccacg aagggaaaga gggctttacc gaattgacat
    16741 tacgtaccct ggaaagtcgt acaccactac tgcgctgcca tcgtcagtat ctggttaatc
    16801 tcgcgcattt acaggagatt cgtctggaag ataacggcca ggccgagttg attttgcgta
    16861 atggcttaac cgtgccggtc agccgccgtt atctgaaaag cttaaaagag gcgattggcc
    16921 tgtaaaagac tgctaaaatg gctttttgcc tcatcaacac ctgaaggcct catgctaagt
    16981 aacgatattc tgcgcagcgt gcgctacatt ttgaaagcca ataataatga cctggtgcgt
    17041 attctggcgc tgggtaatgt cgaagccacc gcggaacaga tcgccgtctg gctacgtaaa
    17101 gaagacgaag agggttttca gcgttgtccg gacattgttt tgtcgtcatt cctcaacggc
    17161 ctgatttatg aaaaacgcgg caaggatgag tctgctccgg cactggagcc ggaacgtcgc
    17221 attaataaca acatcgtgct gaaaaaatta cgcatcgcgt tttcgctgaa aaccgatgac
    17281 attctggcta tcctcaccga acagcagttc cgcgtttcga tgccggaaat tacagcgatg
    17341 atgcgcgcac cggatcataa aaacttccgc gaatgcggcg atcaattttt acgttatttt
    17401 ctgcgtggac tggcagcgcg ccagcatgtg aagaaaagct aagacgggta tggcggccat
    17461 gcgaaacatg gccgccgaca gattatttca cttctttaaa accagcggct ttcatcacca
    17521 gttccatttg cgccatagtg ataccttttt tggcatcttc agcagaaacg ttgattcctg
    17581 aaataccctg cagggcttta aaatccactt tttccatatc gatagtcacg ttttcctgcg
    17641 cgtaggtatc tgtataggtt aatttttctt caacacccgc gatgtttttg tatttggcgc
    17701 ttaacggctc aagtgtcttg gcagcgtctt ctttggtggt tgcaccaata gaagcaaatt
    17761 gaattttggt ttcagaagat tgcttaagca ccttgtcacc tttgtagaca taggtaatgg
    17821 caatttcagt gccgttcaga ttggcgctga atttcttcga ttcttctttg tcaccgcagc
    17881 cagcaagaga gaaaaccaga acagatgcaa caacgaggga aaacagctta ttgaaagcct
    17941 tcatgtaaaa ctccatttta tttaatcaag aaactggtga ctctcaccag gggctatata
    18001 gaatatgcct aataccgtga cgtgagcagt ccggaactgg agtagaactc ttagtaaaaa
    18061 gcactatttc atccttgttg ctgaagcatg gggaataatt gttcgcaaag taaaacaccg
    18121 ttattcattg cttctacccg tgcctcgctt tctgtattac gaaattgtcc caacacatgt
    18181 gccagccgat aaaaaccgac cgcggagagg tcattcgcca gcaactctgc ctgactaata
    18241 gcgctctgtt cctgatagcg ccagccgtta tggagcagtt gaataagtaa cgcctggcag
    18301 cgcatcagca actgatgagc ggtagacggc acaggcaaaa cgctggcaga aggtagcggt
    18361 gccacaggcg cagtttccgc gtccagcgcc caggcgcggg tttttgtcat catcacccgt
    18421 ggttccagtg tcaattgccc atcaacaaaa ctgacaaagc cagaaaccag actcacgggg
    18481 tcgtctgttt gttgcaaaag cgccgccatg cgttcaacgg caaaaggaga acaggcagat
    18541 gccggaaggg ataacgtcag gagattatct tcaccttcgc cgctgattac ctgcgcatcc
    18601 agcgtctggc ggctgctatc ccaaccgagc gaaatacact cagcgaccgg cagaataaat
    18661 aagttatcga cctgattaag aggccgtatg caggcggggg gacgctggcg taaatattcc
    18721 cgcaaagcca caatgcccgg ctggcgtaac ggcgcgctca acatttgcca ggcatcaggc
    18781 gacagcggca caacgctgct taagcggttg cgggtagcta acagcagctc gccatcggca
    18841 ctgcgttttg ctgcttgtga aacaatttgc ccacccgcca gtgcgccagc ctgaaaacta
    18901 aacagccgac gcgtagctgc cggtgagttt tcctgttcac ttcgcggcca actgcgcgaa
    18961 aggtgcaaaa tactgccggt gtcgggatcg gtaaaccaga tgcgtaaacc ataatgctca
    19021 atatcctgcc agcaacgcat acctaaagac accagccgca gatgatcaag ctttgcttct
    19081 ccggcaatgc cagagccaac gaccgtgcgc cacggcacag gaggaacttc accaacactg
    19141 tcgcgccggg ccatctcttg tgcgcaattt aatcgactgt ttaatgccgc aagctgacgt
    19201 aagcattctc cggcatgata gtggctggcg cgagtgtgga aggcatcaac gcttgcccgt
    19261 agctgtcgta gtgattcact cacccagcgc cagttgcagg cctctgccgc ctgcaatgcg
    19321 cggttgaatg ctgcctcgta gtgaataagc ggctggctga tgccgccaag ccataatgcc
    19381 tggcttaatt gctgaacata ttgacgacac gtttggcctt cttcgctggc aaacggatcg
    19441 tcagatgatg tgacgtgttc gctgcgcatc tgccagatta aatggttaaa ttctgcttgc
    19501 tgcgctttgg cctcgacgaa ggcctgcaca gccagtacga catgttcgca aagtgtgcct
    19561 tcaatacaat cacaacgggc gaaacgaata ctgctacggg aataaaaacg cacatcgctc
    19621 atcggtaagc gggcagaggg aatttcgccc ggcgcacaga acaactcaat ggtgatgcct
    19681 ttagcgacca gcgcctgcgc gcgtttgcgg gtggcatcgg gcagggtagc cagttcttcc
    19741 agccagattg ccggatccca ctcttcttct ttttccgtgg gctggacggt ggcacaaagt
    19801 cgttgataac ttaacaccag catcacgcga tgacggcaca tgccgctggc cccgcagctg
    19861 cactgagcct ctttcagtgc ctggctgttc gccagctggg tacggacacc gtcactgaag
    19921 gtggcgatta aagcgccgtt ctcatggctg atttccggga cgttgccatt ttccagttcc
    19981 ttaagactgc gcttaacaaa accggcattg cttaacgccg tcagtgcctg cggtgtcagt
    20041 tctaataatt ccggacgtag tgaattcatg actgaagatt ctccgcaagc catgatgcca
    20101 gctcgcccgg cgtcatggcg gctatttgtg cgccgacatt aaccagcgcc tgggccgtat
    20161 cgcggtcata gcaaggcgtt gcggtgctat cgagcgctgc cagtcccagc actttgatgc
    20221 cgctctggac acactttttc acctgatgcg tcagcaatga tgatgaaccc ccttcgtaaa
    20281 aatcgctcac gaggataatg acgctttttg ctggttgttc aataagttgc cgaccatact
    20341 ccacggcact ggcgatattg gtcccgccgc ccaactgtac tttcattaat aactctaccg
    20401 gatcggcaac gtctgccgtg agatcaacca cgcttgtgtc aaacgccacc agatgggtac
    20461 gaatgccggg taactgccac aaacaggccg ccatcaccgc agagtggatc accgagtcca
    20521 ccatcgatcc gctttgatca accagtaaga ccagttgcca ttgttcgctt tggcgtttaa
    20581 tgcggctgtt aaagcggggg gattcgatat acaacttgcc gtgttgcggg tgccagtgtt
    20641 gcaggttggc gcgcagagta cttttgaaat caaagtttcg cgccagtgga attaatgagc
    20701 ggcgacggcg atcgcggaca ccagaaaaag cctgacgaac ttcctttgcc agtcgcgcca
    20761 taatttcttc aacaacctgg cgcactatct ggcgggcggc agccagtact tcgggattca
    20821 tcagatgttt ggtgtgcaaa acggcgcgta gcaggctttc cgaaggctgc atacgttcca
    20881 gcacgtcgag attcgtcacc acatcttcaa tgccgtagcg cagtacggca tcgctttcca
    20941 gtcgctcaat cacctgttgc ggaaacagag tgtgaatact gttgatccac tcaggggtgg
    21001 tgagatttga gccacctaat ccaccagagc gttcaccacg ctggagccgt tcaggatcgc
    21061 gcccatacag ccactccagc gcgtggtcta tctgccgggc gttgtcatcc agcccacaaa
    21121 gcgtcgtttc tgccgcttcg ccaagaatta atcgccagcg ttgtagctca cgggtggtca
    21181 gaagatcgtt cagttcagac atggaaaact ccccagtttt gcagcgatgc cagtgcctgc
    21241 tgttcgagtt gttgatgatg tgcaatggcc tgtggtggac aatgcaacgg catttgcagc
    21301 gccgaaacgg gaagttgcgc cagttgataa tgctcaagca cctgatgcgc cagcgtcccg
    21361 cgttctcgtg gtggtagcca ggccatcgcc gcgcgtaaat cgggcagggc attgataaaa
    21421 tcggcatcac ttagttgatt taaatgactg ctgaaaccgg cgataaatgt cggctgacag
    21481 gccagttgat ggcgggccag cgccagcaaa ccgtgcagcg cttcgccgga ttgtgccggg
    21541 gataactgcg ccagcatcgt cagcgccgct tcggcactgg cgttaggatg ttccagacgc
    21601 atcagtgcgc catgagccgc gccgcgatcc agcgccgggg catgaattgc ctgactgcgt
    21661 cgctccagta aggctaccgc cgcagaaaga gaaatgccgg ggagttgcac accgctatgt
    21721 agatcgcgca ggatatgaca caacgcttgc cagctatgca ggtgagcgtg aaactccttt
    21781 tcatctggtc tgccgttaga ttcacatagc cacagcgtgc gatcgatagc cgcgcataac
    21841 gtcgtttgta atatctgcgc gccttgcata ccgctaattt catccagccg ccataaggca
    21901 tatagcactt ccagcgcctg gcccatttcg gcaaattgat tttcctgggc gattaataat
    21961 gtgagttgct ccagtaattg ttgactgaag gacgccagac ccgctaacgc cgcctggcta
    22021 agacatgtgg tgatactgcc gattccaccc gcgtccagca tatcggcttc taatttatgg
    22081 cgtgcggctt cctgaagtgt tgcaccaaag catgcggcct caatcaatgc cgcatgctgg
    22141 ctaagcgggc gggttaattt ccagtgttct tcaccgttgc ctgcaagtgt cagtgtactt
    22201 ccctgctggc gtacaatccc agggatctcc agtattgcca gccgatgtaa cacctgactt
    22261 tgcgccagcc catcgggggt aaagcgattt aaggtaagct ccgccggaag agagattgcg
    22321 gtacgttcca gttcgcaggt gacatctttt ggcagaggcg gttgtggcgt ggaaggggca
    22381 agtttaccga atccgtcacc cgctaatgtg tctatcaacg ttagcagaat cggatcggta
    22441 tcgggatgaa taacgccgcg atagctccac ggcaacggtg cattcagggc ttctttaatc
    22501 aacgagcctg ctatggcatc cagccagtca gtgcgtaacg gtagtgtatg accgcgcaac
    22561 tgtgccagtg ccattgcatg cagatgtgcg gcagccatat ccgcggtcga agcaggcaag
    22621 ttgtgctggc gcaaacgggt gagaaccgtt tttagcagtt gttcaccggc ctgctgtaag
    22681 ccccactgcc agcaccagtt ttgccagacc ggggcaggca taccggaaag gtatcctgcc
    22741 agcacatcaa ggcgcttttc actgtagggc gtgagataac aacctgtaat ggcatctgcc
    22801 agcgagggca attccggcgt gttaatgtcc tgcgggcatt cgcgccacat ttttgccagt
    22861 gccggagcgt gccagccacc gcagacgact aacacgtcgc cattattttg ctgcaccgcc
    22921 catgcaatcc agcgagccat aaaggcttcg cgctgacgat tgagtgcatc gccggggaaa
    22981 tcgccccgca gttgggcaaa atagtgcgcc agcgcagagg gtaatgcagt ttgctggctt
    23041 tcatcttcga acaagtgatc ccacagggta tcgctgttat ccatgcgggt ggcacgcagc
    23101 agtaaggcct ggctttcatc ttgcgtatca ggcgaatcgt cctcttcttc actctgcgcc
    23161 cagcaaggca aatcgatgaa gtaagtttgt gcctgaatac gacgtgcggc ttgtagcgcc
    23221 tgccactccg gcgaaaattc agcaaatggc gtccaggcac cacgcccggg ggctgcaccg
    23281 tcctgatact ggcaataact gtaaatagct accggaagct ggtgggaaaa aaaaagttcg
    23341 tctacccggt cattaaaatc agccgggcct tcaatcaaca cgtatcgtgg ccgctggctt
    23401 tcgattaacg atttcaccag ccgggcgcag gccggactat gatgccggat gccgacaatt
    23461 aacggctcgc tcatgcctct ttcctcacgg caggcggtgg cgggcttgat aataagcctg
    23521 ccagtgagct tctttatgtg tggcgacacg ctgttcgaag taacggcgca gacgagcgcg
    23581 atcttcttcg ttatctttga cgatggttcc ggcaatacag tccaccaggt cggcaggctc
    23641 ccccgcgcga ttcgctaaaa accaggcgcg gacgcctacg gcgtgggcca cgttcacggc
    23701 ttcagcggtg gacataatcg ctgtcagtgt atccattgac gttttctttt cgccgttggc
    23761 acgcagatcg cggaaggtgc gtaccagtaa ctccagtact gcgtcaggca ctttatgcgg
    23821 aataccgcta tgggccagca aacgcgccga agcactggcg accagttcca gctcctgggc
    23881 gaaatccata atcggaaaga cggtttcaaa atcgaagcga cgctttagcg cggcactcat
    23941 ctcattgacg ccgcgatcgc gggtattggc agtggcaata atattaaagc cttcccgcgc
    24001 atagagctga ctggcttcac cggtgagttc cggcaccgtc atcaccctgt cagaaagcat
    24061 acctaacaga cagtcttgta cttccagcgg tgtacgcgtt atctcttcaa aacggacgat
    24121 tttgccgtcg cgcattccct ggtaaagcgg ggcagggacc agtgcttccg ttgacggacc
    24181 atggttgata agcagggcgt agttccagcc gtacttgatt tgatcttcgg tagtagatgc
    24241 gccgccctgg atggttaacc cggcatcgcc gctaatagcg gttgccagta attcagacag
    24301 gagagatttt gcggtgccag gttcccccac caacatcaaa ccgcgcccgg tggcaagagt
    24361 gactaacatg cgttcgacag cggagggact aatcaccact ttacggctaa tattttgcgc
    24421 gctatcgcca aggataaaag cacgggccgc aggcaaactt aactgccaac cgggcgggca
    24481 aggtgcgtta tcattttgtt ttaattttgc cagttcatcg gcgtataaca cagcagcagg
    24541 cggacgctgc agatgattat tctgcggtga catatattca tccctgaaat gtattaatga
    24601 aataaacgat attttacgcg caataaatgt ttggcgcatt agttgtttga gcgaaaaata
    24661 tctggaaagc agatcacagt aaataaaaaa acctgctggt gaggcaggtt cttttattta
    24721 ctactttcag gcctaacacc acagcctgtg caacatcgtg ttccaacgaa cgaattataa
    24781 ccacggctgg accccgttgc aattaagata atgagattta taatccagac cgttgggacg
    24841 ttgcgccgga tcgaatctat ttcaagtcct ggctaccgga cgggtggtgc tgcaggtggt
    24901 gccctgatcc ggagtgcaac atcgtggtcc aacatacgta aaccagtatc gacaggcagt
    24961 gtaagggtaa ggtgctggtg ttgcgctcct taat
//
LOCUS       DAPHDW010000059        24930 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.059, whole genome shotgun sequence.
ACCESSION   DAPHDW010000059 DAPHDW010000000
VERSION     DAPHDW010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24930
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.059"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            425..1564
                     /gene="wza"
                     /locus_tag="RH057_002389"
     CDS             425..1564
                     /gene="wza"
                     /locus_tag="RH057_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HDV3128746.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALGNAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            1570..2013
                     /gene="wzb"
                     /locus_tag="RH057_002390"
     CDS             1570..2013
                     /gene="wzb"
                     /locus_tag="RH057_002390"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HDV3128747.1"
                     /translation="MFNNILVVCVGNICRSPTAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMEKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYRKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            2016..4178
                     /gene="wzc"
                     /locus_tag="RH057_002391"
     CDS             2016..4178
                     /gene="wzc"
                     /locus_tag="RH057_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HDV3128748.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITAVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEDEKAKLNGRVTAMPKTQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIVDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGIKRYKQSQLLAMGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            4356..5195
                     /gene="wcaA"
                     /locus_tag="RH057_002392"
     CDS             4356..5195
                     /gene="wcaA"
                     /locus_tag="RH057_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
                     /protein_id="HDV3128749.1"
                     /translation="MKDNPLISIYMPTWNRQQLAIRAIKSVLRQDYSNWEMIIVDDCS
                     TSWEQLQQYVTALNDPRITYIHNDINSGACAVRNQAIMLAQGEYITGIDDDDEWTPNR
                     LSVFLAHKQQLVTHAFLYANDYVCQGEVYSQPASLPLYPKSPYSRRLFYKRNIIGNQV
                     FTWAWRFKECLFDTELKAAQDYDIFLRMVVEYGEPWKVEEATQILHINHGEMQITSSP
                     KKFSGYFHFYRKHKDKFDRASKKYQLFTLYQIRNKRMTWRTLLTLLSVRNGKRLADGI
                     RGR"
     gene            5198..5686
                     /gene="wcaB"
                     /locus_tag="RH057_002393"
     CDS             5198..5686
                     /gene="wcaB"
                     /locus_tag="RH057_002393"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HDV3128750.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            5683..6900
                     /gene="wcaC"
                     /locus_tag="RH057_002394"
     CDS             5683..6900
                     /gene="wcaC"
                     /locus_tag="RH057_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HDV3128751.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDATLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDNIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            6875..8092
                     /gene="wcaD"
                     /locus_tag="RH057_002395"
     CDS             6875..8092
                     /gene="wcaD"
                     /locus_tag="RH057_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HDV3128752.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISVKKLMIALGIGAGLTAFNYLFGQSLDASKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDAMILAGIILSGSFSGV
                     MTFILFYLLEWAFQYLNKEAIKKKLPLALISLAVFLVGVVIAFPYISTRLGDLGTEGS
                     SSYYRIVGPLVMVGYSLTHIDGVVRFGSLYEYVASFGIFNGADVGKTIDNGLYLLIIY
                     FSWFAVFLSLWYMGKVIKMMINAFGDNRNFRVQLYLFTPVSLFFTGSIFSPEYAFLIV
                     CPFILRKALNITR"
     gene            8103..8849
                     /gene="wcaE"
                     /locus_tag="RH057_002396"
     CDS             8103..8849
                     /gene="wcaE"
                     /locus_tag="RH057_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaE"
                     /protein_id="HDV3128753.1"
                     /translation="MLLSIITVAFRNLEGIVKTHASLAHLALAEDISFEWIVVDGGSN
                     DGTREYLENLNGIYNLRFVSEPDNGIYDAMNKGITMAQGKFALFLNSGDIFHQDAANF
                     VRKLKMQKDNMMITGDALLDFGDGHKIKRSAKPGWYIYHSLPASHQAIFFPVSGLKKW
                     RYDLEYKVSSDYALAAKMYKAGYAFKKLNGLVSEFSMGGVSTTNNMELCADAKKVQRQ
                     ILHVPGFWAELSWHLRQRTTSKTKALYNKV"
     gene            8865..9413
                     /gene="wcaF"
                     /locus_tag="RH057_002397"
     CDS             8865..9413
                     /gene="wcaF"
                     /locus_tag="RH057_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HDV3128754.1"
                     /translation="MQDLSGFSVPKGFRGGNAIKVQLWWAVQATIFAWSPQVLYRWRA
                     FLLRLFGAKIGKNVVIRPSVKITYPWKLTLGDYAWVGDDVNLYTLGEITIGAHSVISQ
                     KSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVAPGVTIGDGTVVGARSSVFKT
                     LPANVVCRGNPAVVIRERVETE"
     gene            9440..10561
                     /gene="gmd"
                     /locus_tag="RH057_002398"
     CDS             9440..10561
                     /gene="gmd"
                     /locus_tag="RH057_002398"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HDV3128755.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            10564..11529
                     /gene="fcl"
                     /locus_tag="RH057_002399"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             10564..11529
                     /gene="fcl"
                     /locus_tag="RH057_002399"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HDV3128756.1"
                     /translation="MSKQRIFIAGHRGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     LFLGSSCIYPKLAKQPMAEGELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGCDYRS
                     VMPTNLYGPHDNFHPNNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRG"
     gene            11532..12011
                     /gene="gmm"
                     /locus_tag="RH057_002400"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             11532..12011
                     /gene="gmm"
                     /locus_tag="RH057_002400"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HDV3128757.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPMTAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASDDVHANSRAYFLAEKRAGVPGL"
     gene            12008..13231
                     /gene="wcaI"
                     /locus_tag="RH057_002401"
     CDS             12008..13231
                     /gene="wcaI"
                     /locus_tag="RH057_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HDV3128758.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIDKGVAAENVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADRLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            13234..14670
                     /gene="cpsB"
                     /locus_tag="RH057_002402"
     CDS             13234..14670
                     /gene="cpsB"
                     /locus_tag="RH057_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HDV3128759.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSAGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMERTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            14863..16233
                     /gene="cpsG"
                     /locus_tag="RH057_002403"
                     /gene_synonym="manB"
     CDS             14863..16233
                     /gene="cpsG"
                     /locus_tag="RH057_002403"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HDV3128760.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLRDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            16288..17682
                     /gene="wcaJ"
                     /locus_tag="RH057_002404"
     CDS             16288..17682
                     /gene="wcaJ"
                     /locus_tag="RH057_002404"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HDV3128761.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLIKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            17684..19162
                     /gene="wzxC"
                     /locus_tag="RH057_002405"
     CDS             17684..19162
                     /gene="wzxC"
                     /locus_tag="RH057_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HDV3128762.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDAI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSVVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCVVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            19438..20718
                     /gene="wcaK"
                     /locus_tag="RH057_002406"
     CDS             19438..20718
                     /gene="wcaK"
                     /locus_tag="RH057_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HDV3128763.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHAEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMKQHNSAAGVVGRVKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRSNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPALNARLSEAVSRERQTGMQMVQSVLERIGEVK"
     gene            20715..21935
                     /gene="wcaL"
                     /locus_tag="RH057_002407"
     CDS             20715..21935
                     /gene="wcaL"
                     /locus_tag="RH057_002407"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HDV3128764.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTQN
                     THAAWTKYNLAARTRWLQDEPTGKVAKLRHRASQTLRGIHRKNTWQALNLKRYGAESR
                     NLILSAICGQVATPFYADVFIAHFGPAGVTAAKLRELGVIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGMAFRYRILGIGPWERRLRTLIEQYQ
                     LEDVVEMPGFKPSHEVKAMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLVPENDARALAQRLAAFSQLDTDELAPVVKRAREKVEHDFNQ
                     QVINRELASLLQAL"
     gene            21946..23340
                     /gene="wcaM"
                     /locus_tag="RH057_002408"
     CDS             21946..23340
                     /gene="wcaM"
                     /locus_tag="RH057_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HDV3128765.1"
                     /translation="MPFKKISRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFGEGQTVVVPAGLVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QATGEEGGSMHNITLDVRGSDCTIKGLAMSGFGPVTQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGTDCRQLIHVENGKHFVIRNIK
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVALTNIEMKRASLELHNKPQHLFMRNIKV
                     MQESSIGPALSMNFDMRKDIRGVFMAKKETLLSLANVHAVNEKGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            23515..24408
                     /gene="galF"
                     /locus_tag="RH057_002409"
     CDS             23515..24408
                     /gene="galF"
                     /locus_tag="RH057_002409"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HDV3128766.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVTIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
BASE COUNT         6121 a         6212 c         6761 g         5836 t
ORIGIN      
        1 tgacacttaa tataattctt aaaaatagcc aattgccgaa ttgttacctt gcctgctatt
       61 ccgttagctg taacacttcc tcctgcatta ttggagagcc aatattcaat taccataatg
      121 tcaccttata ttcggaagtt aatttccgca ataacccttt ctcatcaacg attgtatgca
      181 gagagggttt gaaaaattaa gctaaatcag tgtattggta gctaaaaagc caggggcggt
      241 agcgtgtctg gatgcctgaa agaccagtct gaattattct gtcaatagcc tgcggatgaa
      301 atcaattttt ttaggactga tgccagttaa atttgtattc aattaactgc aaggtaaatg
      361 tgcatggcga cattgctatt aatagtgcac aggataatta ctctgccaaa gtgataaata
      421 aacaatgatg aaatccaaaa tgaaattgat gccattattg gtgtcagtaa ccttgataag
      481 cggttgcaca gtacttccgg gcagcaatat gtcgacgatg ggcaaagacg tcatcaaaca
      541 gcaggacgct gatttcgatc tcgacaaaat ggtgaatgtt tatccgctga ccccgcgcct
      601 gattgaccaa ttacgcccac gcccgaatgt agcgcgcccc aatatgacgc tggaaagtga
      661 gatcgcgaat taccagtatc gcgtcgggcc gggcgacgtt ctgaatgtca ccgtctggga
      721 tcacccggaa ctcaccacgc cagccggtca gtaccgcagc tccagcgaca ccggcaactg
      781 ggtacagcct gacggcacta tgttttaccc gtatatcggc aaggtccacg tagtcgggaa
      841 aacgctcgct gaaatccgca gtgatattac cgggcgctta gcgacgtaca tcgctgaccc
      901 gcaggtggat gttaatatcg ccgccttccg ctcgcaaaag gcctatatct caggtcaggt
      961 gaataaatcc ggtcaacagg cgatcaccaa cgtaccactg accattctcg acgccatcaa
     1021 cgccgcaggt ggcctgaccg acaccgctga ctggcgcaac gtggtgctaa cacacaatgg
     1081 tcgtgaagaa cgcatttctt tgcaggcgct gatgcaaaat ggcgacctca atcagaaccg
     1141 actgctttac cccggcgata ttctctacgt gccgcgtaat gatgatctga aagtgtttgt
     1201 gatgggtgaa gtgaagaaac agagcaccct gaaaatggac tttagcggca tgaccctgac
     1261 tgaagccctg ggcaatgccg aaggtatcga catgaccacc tccaacgcca gcggcatctt
     1321 tgtcattcgt ccgctgaaag gcgagggcgg gcgtaacggc aagattgcca atatctacca
     1381 gctggatatg tccgatgcga cgtcgctggt gatggcgaca gaattccgcc tgcaacctta
     1441 tgacgtggtg tatgtcacca ccgccccggt ttcccgctgg aaccgtctga tcaatcagtt
     1501 gctgccaact attagcggtg tccgttacat gacggataca gccagcgaca ttcataactg
     1561 gtaatcgtca tgtttaacaa catcttagtt gtctgtgtcg gcaatatttg ccgttccccg
     1621 acggcggaac gcttactgca acgttatcac ccggagctga aagtggagtc cgctggactc
     1681 ggcgcgctgg tcggtaaggg cgctgatcct accgctatca gcgtcgccgc agaacatcaa
     1741 ctgtctctgg aaggtcactg tgcacggcaa atcagccgcc gcctgtgtcg caactatgac
     1801 ctgattttga ccatggaaaa gcgccatatc gaacgcttat gcgagatggc acctgagatg
     1861 cgcggcaaag tgatgctgtt tggtcactgg gataacgaat gtgaaatccc cgatccgtat
     1921 cgcaaaagcc gggaaacgtt tgcagcggtg tacacattac ttgaacggtc tgcccgccag
     1981 tgggcgcagg cattgaacgc agagcaggta taagaatgac agaaaaagta aaacaacatg
     2041 ccgctccggt aacgggcagt gatgaaatcg atattggtcg cctggtcggc accgtcattg
     2101 aagcgcgctg gtgggtgatt ggcatcaccg ctgtatttgc cctctgtgct gtggtttaca
     2161 ctttcttcgc cacgccgatt tatagtgccg acgcactggt acaaatcgaa caaaacagcg
     2221 gcaattcgtt agtgcaggac attggctcgg cattagccaa caaaccgcct gcatcggacg
     2281 ccgagatcca gttgattcgt tcgcgtctgg tgcttggtaa aacggtggac gatctagacc
     2341 tcgatattgc ggtgagcaaa aacacgttcc cgattttcgg tgcgggttgg gatcgcctga
     2401 tgggacgtca gaacgagacg gtgaaagtga ccacctttaa ccgcccgaaa gagatggcgg
     2461 accaggtgtt tacgcttaat gtgctggatg acaaaaacta cactctgagc agtgatggcg
     2521 gctttagcgc ccgtgggcaa gcgggccaga tgctgaaaaa agaaggcgtc acgctgatgg
     2581 ttgaagccat tcacgcccgc ccgggcagtg agtttaccgt caccaaatac tccacgttgg
     2641 ggatgatcaa ccaactgcaa aacagcctga cggtaacgga gaacggcaaa gacgcaggcg
     2701 tactgagcct gacttatacc ggtgaagatc gcgaacagat ccgcgacatt cttaacagca
     2761 tcgcccgtaa ctatcaggaa caaaatattg agcgcaaatc ggcggaagcg tcgaaaagcc
     2821 tcgctttcct cgcgcaacag ttaccggaag tacgtagccg cctcgatgtt gccgaaaaca
     2881 aactgaatgc cttccgtcag gataaagatt ctgttgatct gccgctggaa gcgaaagcgg
     2941 tgctcgattc gatggtgaac atcgacgcgc agttgaacga actgaccttt aaagaggcgg
     3001 aaatctccaa gctgtacacc aaagttcacc ccgcgtaccg cacgctgctg gagaaacgtc
     3061 aggcgctgga agacgaaaaa gccaaactta atggtcgcgt aacggcgatg ccgaaaaccc
     3121 agcaggaaat tgtccgtctg acccgcgatg tcgagtctgg tcagcaggtc tatatgcaac
     3181 tgctgaataa agagcaggag ctgaaaatca ccgaagccag caccgtcggc gatgtgcgca
     3241 ttgttgaccc ggcaatcacc cagcctggcg tgctgaaacc gaagaaaggg ctgattatcc
     3301 tcggcgcgat tatccttggc ctgatgcttt ctatcgtggg tgtgctgctg cgctcgttgt
     3361 ttaatcgcgg catcgaaagc ccgcaggtgc tggaagaaca cggtatcagc gtctatgcca
     3421 gcatcccgct gtcggaatgg cagaaagcgc gcgatagcgt caaaaccatc aaagggatta
     3481 aacgctataa acagagccag ctactggcga tggggaatcc aaccgatctg gcgattgaag
     3541 ccatccgcag tctgcgtacc agtctgcact tcgcgatgat gcaggcacag aacaatgtgt
     3601 tgatgatgac cggggttagc ccgtcaatcg gtaaaacctt tgtctgcgcc aacctggcgg
     3661 cggtgatcag ccagaccaat aaacgggtgt tgttgatcga ctgcgatatg cgcaaaggct
     3721 acacccacga gctgctgggc actaataacg ttaatggcct gtcggaaatt ctgattggtc
     3781 agggcgatat tactaccgct gctaaaccga cctctattgc caaatttgac ctgatcccgc
     3841 gcggtcaggt gccgccaaat ccttctgaac tgttgatgag cgaacgcttt gccgaactgg
     3901 tgaactgggc gagtaaaaac tatgacctgg tgttgattga tacgccgccg attctggcag
     3961 tgaccgatgc ggcaattgtt ggtcgtcatg tcggaaccac gttaatggtg gcgcgttatg
     4021 cggtcaacac cttgaaagaa gtggaaacca gtctgagccg ctttgagcaa aacggtattc
     4081 cggtgaaagg ggtgattctg aactccatct tccgccgcgc cagcgcgtat caggattatg
     4141 gctattacga atacgaatat aagtcggatg cgaaataacg acatccgctg tcggatgcga
     4201 tgctgacgca tcttatccga cctaccttca cgccgcatcc ggcaagcaaa ccagctccgt
     4261 aggccggata aggcgctcac gccgcatccg gcaacagaac ccgtaaacaa gcttataaac
     4321 cggcagaaca tctcgacact ccgggagaac aacctatgaa agacaatccg ctgatttcaa
     4381 tctatatgcc gacctggaac cgccaacaac tggcgattcg ggcgataaaa tcggtcctgc
     4441 gccaggacta cagcaactgg gagatgatca tcgtggatga ttgctccacc tcctgggaac
     4501 aactgcaaca gtacgtcact gccctcaacg atccgcgtat tacctacatt cacaacgaca
     4561 ttaacagcgg ggcgtgcgcg gtacgtaacc aggcgattat gctggcgcaa ggggaataca
     4621 ttaccgggat cgatgacgat gatgaatgga cgcccaaccg cctgagcgtc ttcctcgccc
     4681 ataaacagca actggtcact cacgcctttt tgtacgctaa cgactacgta tgccagggag
     4741 aagtttattc ccaaccagca agcttaccac tgtatcccaa atcgccgtac tcacgccgcc
     4801 tgttctataa acgcaatatc attggcaatc aggtttttac ctgggcgtgg cgtttcaaag
     4861 agtgcttgtt cgataccgaa ctgaaagcag cacaggatta cgacatcttc ctgcggatgg
     4921 tggtggagta tggcgaaccg tggaaagtgg aagaggcgac gcagatcctg catatcaatc
     4981 acggtgaaat gcagatcacc tcgtcaccga aaaaattctc cgggtacttc cacttttacc
     5041 gcaagcacaa agataaattt gaccgtgcaa gcaaaaaata tcagctgttt accctctacc
     5101 agatccgcaa taaacgtatg acctggcgca ctttgctgac gctgctgtcg gtgcgtaacg
     5161 gcaaacgtct ggctgatggt attcgggggc gctaaagatg ctggaagatc tgcgcgccaa
     5221 cagctggagt ttacgcccgt gctgcatggt tcttgcctat cgcgttgctc atttttgttc
     5281 ggtgtggcgc aaaaagaacg tcctcaacaa tctgtgggcg gccccgctgc tggtgctgta
     5341 tcgcattatc accgaatgct ttttcggtta tgaaatccag gccgccgcga ccattggccg
     5401 ccgctttact atccatcacg gttacgccgt agtgatcaat aaaaacgtgg tggcggggga
     5461 tgatttcacc attcgccacg gcgtcactat cggcaatcgt ggtgccgata acatggcatg
     5521 tccacacatt ggcaacggcg tcgaactcgg tgccaacgtc attattcttg gtgatatcac
     5581 gcttggtaac aacgtcaccg tgggtgcggg cagcgtggtg ctcgactctg tcccggacaa
     5641 cgcgctggtg gtgggagaaa aagcgcgagt gaaggtaatt aaatgaatat tttgcaattt
     5701 aatgtgcgac tggcggaagg cggggcagca ggtgtggcgt tagatctcca ccagcgtgcg
     5761 ctgcaacagg ggctggcgtc acattttgtc tacggttacg gcaaaggcgg caaagagagc
     5821 gtcagccatc aaaactatcc gcaggtcatc aaacatacgc cgcggatgac cgcgatggcg
     5881 aacattgccc tgtttcgtct gtttaatcgc gatctgttcg gcaatttcaa tgagttatat
     5941 cgcaccatta ctcgcacacc gggtccggtg gtcctgcatt ttcatgtgct gcacagctac
     6001 tggttgaatc ttaacagcgt ggtgcgcttt tgcgaaaagg tgaaaaacca taaaccggac
     6061 gccactctgg tctggacgct gcacgatcac tggagcgtta ccggacgctg cgcctttacc
     6121 gacggttgcg aaggctggaa aacgggctgc cagaaatgcc cgaccttaaa taactatccg
     6181 ccggtgaaga ttgatcgggc gcaccagctg gtggcgggca aacgccagtt attccgcgag
     6241 atgctggcgc tgggctgtca gtttatttcc cccagccagc atgtggctga cgctttcaat
     6301 agtctgtacg ggccagggcg ttgccggatt atcaataatg gtattgatat ggcaaccgaa
     6361 gcgattctgg cggacttgcc tccggtgcgc gaaacccagg gcaagccgaa aatcgcggtg
     6421 gtggcgcatg acctgcgtta cgacggcaag actaaccagc aactggtgcg tgagatgatg
     6481 gcgctgggcg acaacattga actgcatacc tttggtaagt tctcgccgtt caccgctggc
     6541 aacgtggtta atcacggctt tgaaactgac aagcgcaagt tgatgagcgc gctcaatcaa
     6601 atggatgcgc tggtgttcag ttctcgcgtc gataactacc cgctgatttt gtgtgaggcg
     6661 ctatcgattg gcgtgccggt gatcgccacc catagcgatg cggcgcggga agtgctgcaa
     6721 aaatccggtg gtaaaaccgt cagtgaagaa gaggtgctgc aactggtgca gttaagcaaa
     6781 ccggaaatcg cgcaggcgat atttggtacc acgctggctg agttcagcca acgcagccgc
     6841 gccgcctaca gtggacaaca gatgctggag gagtatgtca acttctatca gaatctgtag
     6901 ctacctgctg ctgccgctga tctacctgct ggttaacgtc aaaatcgccc agcttggtga
     6961 aagtttcccc atcactatcg tcactttttt acctgtcttg ttactgctgt ttttagaacg
     7021 catcagcgtt aaaaaattga tgattgcctt agggattggt gcgggactca cggcgttcaa
     7081 ctatctgttt ggtcagtcgt tggatgccag taaatacgtc acttcaacca tgctgtttgt
     7141 ctatattgtg atcattattg gcatggtgtg gagtattcgc tttaaaacga tttcgccaca
     7201 caaccatcgc aagatattac gtttctttta tctggtggtc gggctggtgg tggcgctggc
     7261 ggcagtggag atggcgcaaa ttatcctcac cggtggcagc agtattatgg agtcgatttc
     7321 gaaatatctg atttacagca acagctatgt gctgaatttc attaaattcg gcggcaagcg
     7381 cacgacagca ctttatttcg aaccggcatt tttcgctctg gcattaatct caatttggct
     7441 cagcatcaaa cagtttggta tcaaaacgcc taaaaccgat gctatgattc tcgcagggat
     7501 aatattatcc ggatcgtttt caggggttat gacctttatc ctgttttatt tgctggagtg
     7561 ggcatttcaa tatctgaata aagaggcgat taagaaaaag ttgccgttag cattgatttc
     7621 tctggctgta ttcctggttg gtgtggtaat cgcgtttcct tatatttcca cccgtctggg
     7681 cgatttaggt acggaaggat cgtcatcgta ttatcgtatt gtcggtccgc tggtaatggt
     7741 cggttattct ttgacccata ttgacggtgt agtcagattt ggctcacttt atgaatatgt
     7801 cgcatcattc ggaatattta acggtgcgga tgtcggaaaa accatagaca atggtttgta
     7861 tctgctgatt atttattttt cctggttcgc agtgttttta tcgctgtggt acatggggaa
     7921 agtgataaaa atgatgatca acgcttttgg tgataaccgc aattttcgcg tgcaattata
     7981 tctttttact ccggtgtcgc tgttttttac cggttcgata tttagcccgg aatatgcatt
     8041 tttaatcgtc tgtccgttta ttttgcgaaa agcgttaaat attacgaggt aagaataaga
     8101 acatgttgct tagcataatc actgtcgcgt ttcgtaacct cgaagggata gtcaaaacac
     8161 atgcctcgct ggcgcatctg gcgctggcgg aagatatcag cttcgaatgg attgttgtcg
     8221 atggcggttc caatgacggc acccgtgagt atctggaaaa tctcaatggt atctataacc
     8281 tacgctttgt cagcgagcca gataacggta tctacgacgc catgaacaaa ggcattacga
     8341 tggcacaagg caagttcgcg ttgtttctca attcgggcga tatttttcat caggatgccg
     8401 caaattttgt ccgtaagtta aaaatgcaaa aagataacat gatgatcacc ggcgatgcgc
     8461 tgctggattt tggcgacggg cataaaatta aacgtagcgc caaaccgggc tggtatattt
     8521 atcacagcct gcccgccagt catcaggcga tatttttccc tgtatccggc ttgaaaaaat
     8581 ggcgttatga cctggagtat aaagtttctt ccgactacgc gctggcagcc aaaatgtata
     8641 aagccggtta tgcatttaaa aaactcaatg gcctggtgtc tgaattttcc atgggtgggg
     8701 tatctaccac caataatatg gaattgtgtg ctgacgcgaa aaaagtccaa cgacaaatat
     8761 tacatgtacc tggcttttgg gctgaattat cctggcattt acgccaacgt actacctcaa
     8821 agacgaaagc cttatataac aaagtctgaa tataaggaga accaatgcaa gatttaagcg
     8881 gtttctcggt gccgaaaggg ttccggggcg gcaacgctat taaagtgcaa ttatggtggg
     8941 cagtacaggc aacaatattt gcctggtcgc cacaagtatt gtatcgctgg cgggcatttt
     9001 tattacgttt attcggcgca aaaataggaa aaaacgtagt tattcgtccg tcagtaaaaa
     9061 ttacctatcc gtggaaatta accttaggtg attacgcgtg ggtcggcgat gacgtcaatt
     9121 tatataccct cggtgaaata accattggcg cacattcggt gatatcgcaa aaaagttatt
     9181 tatgcaccgg tagccacgac catgcaagtc aacatttcac cattaatgct acgcctattg
     9241 tgattggcga gaaatgctgg ctggcaaccg atgtttttgt tgcccctggc gtcacgattg
     9301 gcgacggcac cgtcgtgggt gcacgaagca gtgtttttaa aacgcttccg gcaaatgtgg
     9361 tttgccgggg gaatcccgca gtggtgatac gcgaacgcgt tgaaactgaa taaattcaaa
     9421 aaatacagag gaataagaca tgtcaaaagt cgctctcatc accggtgtaa ccggacaaga
     9481 cggttcttac ctggcagagt ttctgctgga aaaaggttac gaggtgcatg gtattaagcg
     9541 tcgcgcatcg tcattcaaca ccgagcgcgt ggatcatatt tatcaggatc cgcatacctg
     9601 taacccgaaa ttccatctgc attatggcga cctgagtgat acctccaacc tgacgcgtat
     9661 tttgcgtgaa gtgcagccgg atgaagtgta caacctgggc gcaatgagcc acgttgcggt
     9721 ttcttttgag tcaccggaat ataccgctga cgtcgacgcg atgggtacgc tgcgcctgct
     9781 cgaggcgatc cgcttcctcg gtctggaaaa gaaaacccgt ttctatcagg cttccacctc
     9841 tgaactgtat ggtctggtgc aggaaattcc gcagaaagaa accacgccgt tctacccgcg
     9901 atctccgtat gcggtcgcca aactgtacgc ctactggatc accgttaact accgcgaatc
     9961 ctacggcatg tacgcctgca acggtattct cttcaaccat gaatccccgc gccgtggcga
    10021 aaccttcgtt acccgcaaaa taacccgcgc aatcgccaac atcgcccagg ggctggagtc
    10081 gtgcctgtac ctcggcaata tggattccct gcgtgactgg ggccacgcca aagactacgt
    10141 aaaaatgcag tggatgatgc tgcaacagga acagccagaa gatttcgtta ttgctaccgg
    10201 cgttcagtac tccgtgcgtc agttcgtaga aatggcggca gcacagctgg gcatcaaact
    10261 gcgctttgaa ggcacgggcg ttgaagagaa gggcattgtg gtttccgtca ccgggcatga
    10321 cgcgccgggc gttaaaccgg gggatgtgat tatcgccgtt gacccgcgtt acttccgtcc
    10381 ggctgaagtt gaaacgctgc tcggcgaccc gactaaagcg cacgaaaaac tgggctggaa
    10441 accggaaatc accctcagag agatggtgtc tgaaatggtg gctaatgacc tcgaggcggc
    10501 gaaaaaacac tctctgctga aatctcacgg ctacgacgtg gcgatcgcgc tggagtcata
    10561 agcatgagta aacaacgaat ttttatcgct ggtcatcgcg ggatggtcgg ttccgccatc
    10621 aagcgacagc tcgaacagcg cggtgatgtg gaactggtat tacgcacccg cgacgagctg
    10681 aacctgctgg acagccgcgc cgtgcatgat ttctttgcca gcgaacgtat tgaccaggtc
    10741 tatctggcgg cggcgaaagt gggcggcatt gttgccaaca acacctatcc ggcggatttc
    10801 atctaccaga acatgatgat tgagagcaac atcattcacg ctgcgcatca gaacgacgtg
    10861 aacaaactgc tgtttctcgg atcgtcctgc atctacccaa aactggcaaa acagccgatg
    10921 gcagaaggcg agctgttaca gggcacgctg gagccgacca acgagccata cgccattgcc
    10981 aaaatcgccg ggatcaaact gtgcgaatct tacaaccgcc agtatggatg cgattatcgc
    11041 tctgtcatgc cgaccaacct gtacgggccg cacgacaact tccatccgaa taattcgcat
    11101 gtgatccccg cattgctgcg ccgcttccac gaggcgacag cacagaatgc accggacgtg
    11161 gtggtgtggg gcagcggtac gccgatgcgc gaatttctgc atgtcgatga tatggcggcg
    11221 gcaagcattc atgtcatgga gctggcgcac gaagtctggc tggagaacac ccagccgatg
    11281 ctgtcgcaca ttaacgtcgg cacgggtgtt gactgcacta tccgcgagct ggcgcaaacc
    11341 atcgccaaag tggtgggtta caaaggccgg gtggttttcg atgccagtaa accggatggc
    11401 acaccgcgca aactgctgga tgtgacgcgc ctgcatcagc ttggctggta tcacgaaatc
    11461 tcactggaag cggggcttgc cagcacttac cagtggttcc ttgagaatca agaccgcttt
    11521 cgggggtaat gatgttttta cgtcaggaag actttgccac ggtagtgcgc tccactccgc
    11581 ttgtctctct cgactttatt gtcgagaaca gccgcggcga gtttctgctt ggcaaaagaa
    11641 ccaaccgccc ggcacagggt tactggtttg tgccgggagg gcgcgtgcag aaggacgaaa
    11701 cgctggaagc cgcatttgag cggctgacga tggcggaact ggggctgcgt ctgccgatga
    11761 cagcgggcca gttttacggt gtctggcagc acttttatga cgataacttc tccggcacgg
    11821 atttcaccac gcactatgta gtgctcggtt ttcgcttcag agtcgcggaa gaggaactat
    11881 tactgccgga tgagcagcat gacgattacc gctggctgac gccggacgcg ctgctcgcca
    11941 gtgacgatgt tcacgctaac agccgcgcct attttctcgc tgagaagcgt gccggagtac
    12001 ccggattatg aaaatactgg tctacggcat taactactcg ccggagttaa ccggcatcgg
    12061 caaatacacc ggcgagatgg tggaatggct ggcagcacaa ggtcatgagg tgcgggtcat
    12121 taccgcaccg ccgtactacc cgcagtggca ggtgggcgag aactattccg cctggcggta
    12181 caaacgagaa gagggggccg ccacggtgtg gcgctgcccg ctgtacgtgc caaaacagcc
    12241 gagcaccctg aaacgcctgt tacatctcgg cagttttgcc gtcagcagtt tctttcctct
    12301 gatggcgcaa cgtcgctgga agccggatcg cattatcggc gtggtgccaa cgctgttttg
    12361 cacgccggga atgcgcctgc tggcgaaact ctctggtgcg cgtaccgtgc tgcatattca
    12421 ggattacgaa gtagatgcca tgcttgggct gggccttgcc ggaaaaggca aaggcggcaa
    12481 agtggcacag ctggcgacgg cgttcgaacg tagcggactg cataacgtcg ataacgtctc
    12541 cacgatttcg cgttcgatga tgaataaagc catcgataaa ggcgtggcgg cggaaaacgt
    12601 catcttcttc cccaactggt cggaaatcgc ccgttttcag catgttgcag acgccgatgt
    12661 tgatgcactt cgtaaccagc ttggcctgcc ggataacaaa aaaatcattc tttactccgg
    12721 caatattggt gaaaagcagg ggctggaaaa cgttattgaa gctgccgatc gtctgcgcga
    12781 tgaaccgctg atttttgcca ttgtcgggca gggcggcggc aaagcgcggc tggaaaaaat
    12841 ggcgcagcag cgtggactgc gcaacatgca atttttcccg ctgcaatcgt atgacgcttt
    12901 acccgcactg ctgaagatgg gcgattgcca tctggtggtg caaaaacgcg gcgcggcaga
    12961 tgccgtattg ccgtcgaaac tgaccaatat tctggcggta ggcggtaacg cggtgattac
    13021 cgcagaggcc cacacagaac tggggcagct ttgcgaaacc tttccgggca ttgcggtttg
    13081 cgtagaaccg gaatcggtcg aggcgctggt ggcggggatc cgtcaggcgc tcctgctgcc
    13141 caaacacaac acggtggcac gtgaatatgc cgaacgcaca ctcgataaag agaacgtgtt
    13201 acgtcaattt ataaatgata ttcggggata attatggcgc agtcgaaact ctatccagtt
    13261 gtgatggcag gtggctccgg tagccgctta tggccgcttt cccgcgtact ttaccccaag
    13321 cagtttttat gcctgaaagg cgatctcacc atgctgcaaa ccaccatctg ccgcctgaac
    13381 ggcgtggagt gcgaaagccc ggtggtgatt tgcaatgagc agcaccgctt tattgtcgcg
    13441 gaacagctgc gtcaactgaa caaactcacc gagaacatta ttctcgaacc ggcagggcgt
    13501 aacactgcac ctgccattgc actggcggcg ctggcggcaa aacgtcatag cccggagagc
    13561 gacccgttaa tgctggtatt ggcggcggat catgtgattg ccgatgaaga cgcgttccgt
    13621 gccgccgtgc gtaatgccat gccatatgcc gaagcgggca agctggtgac cttcggcatt
    13681 gtgccggatc taccagaaac cggttatggc tatattcgtc gcggtgaagt gtctgcgggt
    13741 gagcaggata cggtggcctt tgaagtggcg cagtttgtcg aaaaaccgaa tctggaaacc
    13801 gcccaggcct atgtggcaag cggcgaatat tactggaaca gcggtatgtt cctgttccgc
    13861 gccggacgct atctcgaaga actgaaaaaa tatcgcccgg atattctcga tgcctgtgaa
    13921 aaagcgatga gcgccgtcga tccggatctt gattttattc gtgtggatga agaagcgttt
    13981 ctcgcctgcc cggaagagtc ggtggattac gcggtcatgg aacgtacggc agatgctgtt
    14041 gtggtgccga tggatgcggg ctggagcgat gttggttcct ggtcttcatt atgggagatc
    14101 agcgcccaca ccgccgaggg caacgtttgc cacggcgatg tgattaatca caaaactgaa
    14161 aacagctatg tgtacgccga atctggcctg gtcaccaccg tcggggtgaa agatctggta
    14221 gtggtgcaga ccaaagatgc ggtgctgatt gccgaccgta acgcggtgca ggatgtgaaa
    14281 aaagtggtcg agcagatcaa agccgatggt cgccatgagc atcgggtgca tcgcgaagtg
    14341 tatcgtccgt ggggcaaata tgactctatc gacgcgggcg accgctacca ggtgaaacgc
    14401 atcaccgtga aaccgggcga gggcttgtcg gtacagatgc accatcaccg cgcggaacac
    14461 tgggtagtgg tcgcgggaac ggcaaaagtc accattgatg gtgatatcaa actgcttggt
    14521 gaaaacgagt ccatttatat tccgctgggg gcgacgcact gcctggaaaa cccggggaaa
    14581 attccgctcg atttaattga agtgcgctcc ggctcttatc tcgaagagga tgatgtggtg
    14641 cgcttcgcgg atcgctacgg acgggtgtaa acgtcgcatc aggcgctgtt tgtcggatgc
    14701 ggcgtgaacg ccttatccaa cctacggttc gggtttgtag tcctgataag acgcggcagc
    14761 gtcgcatcag gcaatgaatg cgaaaccgag atgtaaataa cgacaaaaac aaaattggcc
    14821 gtttcggtca gggccaacta ttgcctgaaa aagggtaacg acatgaaaaa attaacctgc
    14881 tttaaagcct atgatattcg cgggaaatta ggcgaagaac tgaatgaaga tattgcctgg
    14941 cgcattggtc gcgcctatgg cgaatttctc aaaccgaaaa ccattgtgtt aggcggtgat
    15001 gtccgcctca ccagcgaaac cttaaaactg gcgctggcaa aaggtttacg ggatgcgggc
    15061 gtcgatgtgc tggatattgg catgtccggc actgaagaga tctatttcgc cacgttccat
    15121 ctcggcgtgg atggcggcat tgaagttact gccagccata atccgatgga ttacaacggc
    15181 atgaagctgg tgcgcgaagg ggctcgcccg atcagcggcg ataccggact gcgcgatgtc
    15241 cagcgtctgg cagaagccaa cgactttcct cccgttgatg aaaccaaacg cggtcgctat
    15301 cagcaaatca atctgcgtga cgcttacgtt gatcacctgt tcggttatat caacgtcaaa
    15361 aacctcacgc cgctcaagct ggtgatcaac tccgggaacg gcgcagcggg tccggtggtg
    15421 gacgccatcg aagcccgctt taaagccctc ggcgcacctg tggaattgat caaagtgcac
    15481 aacacgccgg acggtaattt ccccaacggt attcctaacc cgctactgcc ggaatgccgc
    15541 gacgacaccc gcaatgcggt catcaaacac ggcgcggata tgggcattgc ctttgatggc
    15601 gattttgacc gctgcttcct gtttgacgaa aaagggcagt ttatcgaagg ctactacatt
    15661 gtcggcctgc tggcagaagc gttcctcgaa aaaaatcccg gcgcgaagat catccacgat
    15721 ccacgtctct cctggaatac cgttgatgtg gtgactgccg ctggcggcac gccggtgatg
    15781 tcgaaaaccg gacacgcctt tattaaagaa cggatgcgca aggaagacgc catctacggt
    15841 ggcgaaatga gcgcccacca ttacttccgt gatttcgctt actgcgacag cggcatgatc
    15901 ccgtggctgc tggtcgccga actggtgtgt ctgaaaggaa aaacgctggg cgaactggta
    15961 cgcgaccgga tggcggcgtt tccggcaagc ggtgagatca acagcaaact ggcgcaaccc
    16021 gttgaggcga ttaaccgcgt cgaacagcat tttagccgtg aggcgctggc ggtggatcgt
    16081 accgatggca tcagtatgac ctttgccgac tggcgtttta acctgcgctc ctccaacacc
    16141 gaaccggtgg tgcggttgaa tgtggaatcg cgcggtgatg tgccgctgat ggaagcgcga
    16201 acgcgaactc tgctgacgtt gctgaacgag taatgtcgga tcttccctta ccccactgcg
    16261 ggtaaggggc taataacagg aacaacgatg acaaatctaa aaaagcgcga gcgagcgaaa
    16321 accaatgcat cgttaatctc tatggtgcaa cgcttttcag atatcaccat catgtttgcc
    16381 ggactatggc tggtttgcga agtgagcgga ctgtcattcc tctacatgca cctgttggtg
    16441 gcgctgatta cgctggtggt gttccagatg ctgggcggca tcaccgattt ttatcgctca
    16501 tggcgcggtg ttcgggcagc gacagaattt gccctattgc tacaaaactg gaccttaagc
    16561 gtgattttca gtgccggact ggtggcgttc aacaatgatt tcgacacgca actgaaaatc
    16621 tggctggcgt ggtatgggct gaccagtatc ggactggtgg tttgccgttc atgtattcgc
    16681 attggggcgg gctggctgcg taatcatggc tataacaagc gcatggtcgc ggtggcgggg
    16741 gatttagccg ccggacaaat gctgatggag agcttccgta atcagccgtg gttagggttt
    16801 gaagtggtgg gcgtttacca cgacccgaaa ccgggcggcg tttctaacga ctgggcgggt
    16861 aacctgcaac agctggtcga ggatgctaaa gcgggtaaga ttcataacgt ctatatcgcg
    16921 atgcaaatgt gcgacggcgc gcgagtgaaa aaactggtcc atcaattggc agacaccacc
    16981 tgttcggtgc tgctgatccc cgatgttttt accttcaaca ttctccattc acgtctcgaa
    17041 gagatgaacg gcgttccggt ggtgccgctg tacgatacgc cgctttccgg ggttaaccgc
    17101 ctgataaaac gtgcggaaga cattgtgctg gcgacgctga tcttgctgct gatctccccg
    17161 gtgctgtgct gtattgcgct ggcggtgaaa ctcagttcac cagggccggt tattttccgc
    17221 cagactcgct atggcatgga tggcaagccg atcaaagtgt ggaagttccg ttcgatgaaa
    17281 gtgatggaga acgacaaagt ggtgacccag gcgacgcaga acgatccgcg cgtcaccaaa
    17341 gtggggaact ttctgcgccg cacctcgctg gatgaactgc cgcagtttat caatgtgctg
    17401 accggcggga tgtcgattgt cggtccacgt ccgcacgcgg tggcgcataa cgaacagtat
    17461 cgacagctca ttgaaggcta catgctgcgc cataaagtga aaccgggcat taccggctgg
    17521 gcgcagatta atggctggcg tggcgaaacc gacacgctgg agaaaatgga aaaacgcgtc
    17581 gagttcgacc ttgagtacat ccgcgaatgg agcgtctggt tcgatattaa aatcgttttc
    17641 ctgacggtat tcaaaggttt cgttaacaaa gcggcatatt gatatgagct tacgtgaaaa
    17701 aaccatcagc ggcgcgaagt ggtcggcgat tgctacggtg atcatcatcg gcctcgggct
    17761 ggtacagatg accgtgctgg cgcggattat tgacaaccac cagttcggtt tgcttaccgt
    17821 gtcgctggtg atcatcgcgc tggcagatac gctttctgac ttcggtatcg ctaactcgat
    17881 tattcagcga aaagaaatca gccaccttga actcaccacg ctgtactggc tgaacgtcgg
    17941 gctggggctt gtggtgtgcg tggcggtgtt tttgttgagt gatgccattg gtgacgtgct
    18001 gaataacccg gacctggcac cgttgattaa aacattgtcg ctggcgtttg tggtgatccc
    18061 ccacgggcag cagttccgcg cgttgatgca aaaggaactg gagttcaaca aaatcggcat
    18121 gatcgaaacc agcgcggtgc tggcgggctt cacttttaca gtggttagcg cccatttctg
    18181 gccgctggcg atgaccgcga tcctcggtta tctggtcaat agtgcggtaa gaacgctgct
    18241 gtttggctac tttggccgca aaatttattg ccccggtctg cacttctcgc tggcgtctgt
    18301 ggcaccgaac ttacgttttg gtgcctggct gacggcggac agcatcatca actatctcaa
    18361 taccaacctt tcaacgctgg tgctggcgcg tattctcggc gcgggcgtgg cagggggata
    18421 caacctggcg tacaacgtgg ccgttgtgcc gccgatgaag ctgaacccga tcatcacccg
    18481 cgtgttgttt ccggcgtttg ccaaaattca ggacgacacc gaaaagctgc gcgtcaactt
    18541 ctacaagttg ctgtcggtgg tggggattat caactttccg gcgctgctcg ggctgatggt
    18601 ggtgtcgaat aattttgtac cgctggtctt tggtgagaag tggaacagca ttattccggt
    18661 gctgcaattg ctgtgtgtgg tgggtctgct gcgctccgta ggtaacccga ttggctcgct
    18721 gctgatggcg aaagcgcggg tcgatatcag ctttaaattc aacgtattca aaacatttct
    18781 gtttattccg gcgattgtta ttggtgggca gatggcgggc gcaatcggcg tcacgcttgg
    18841 cttcctgctg gtgcagatta tcaacaccat tctgagttac ttcgtgatga ttaaaccggt
    18901 tcttggttcc agttatcgcc agtacatcct gagtttgtgg ctgccgtttt atctctcgct
    18961 gccgacgctg gtggtcagtt atgcgctggg cattgtgctg aaagggcaac tggcgctggg
    19021 gatgctgctg gcggtgcaaa tagccgcggg cgtgctggcg tttgtggtga tgattgtgct
    19081 gtcgcgccat ccgctggtgg tggaagtgaa gcgtcagttt tgtcgcagcg aaaaaatgaa
    19141 aatgctttta cgggcggggt gagttgttgt tttcggttcc gacaacggtt gccgggtgca
    19201 gcgtaaaacg ccttatccat cctacggttc tgtgctcggg acctgtaggc ctgataagac
    19261 gcgtcagcgt cgcatcaggc actgaatgcc ggatgcggcg tgaacgcctt atccggccta
    19321 cggttctgtg ctcgggtctt gtaggcctga taagacgcgc cagcgtcgca tcaggcaaaa
    19381 aacggcaacg aacacaacaa acacagcctc acctggcatc aaatttatga ggaaaaaatg
    19441 aaattactta ttctgggcaa ccacacttgc ggcaaccgcg gcgacagcgc catcttgcgc
    19501 ggcttacttg atgccatcaa catcctcaat ccacacgcag aagtggacgt gatgagccgc
    19561 tatccggtca gttcttcctg gctgctcaac cgcccggtaa tgggcgatcc gctgttcctg
    19621 caaatgaaac aacacaacag cgcggcgggc gttgtcgggc gcgttaaaaa agtcctccgt
    19681 cgccgctatc agcatcaggt attgctctca cgcgtcaccg acactggcaa gttgcgtaat
    19741 atcgccatcg cccagggatt cactgacttc gtgcgactgc tgtcaggtta cgacgccatt
    19801 attcaggtcg gcggatcgtt ttttgtcgat ctctacggcg tgccgcagtt tgaacatgcg
    19861 ctttgcactt tcatggcgaa aaagccgctg tttatgattg gtcacagtgt tggcccgttc
    19921 caggatgagc aatttaacca actggcgaac tatgtttttg gtcactgtga cgcgctgatc
    19981 ctgcgcgaat cggtcagcct tgatttgatg aaacgcagca atatcaccac tgcaaaagtg
    20041 gaacatggcg tcgatactgc gtggctggtc gatcaccaca ccgaagattt caccgccagc
    20101 tatgccgtcc aacactggct ggacgttgcc gcacaacaga aaacggtggc aattaccctg
    20161 cgcgaactgg caccgttcga caaacgtctc ggcaccactc aacaagcgta tgaaaaagcc
    20221 tttgccgggg tggtcaatcg cattctcgac gaaggctatc aggttattgc gctctccacc
    20281 tgtacgggta tcgacagcta taacaaagac gaccgcatgg tggcgctcaa cctgcgccag
    20341 cacatcagtg atcctgcccg ttatcacgta gtgatggatg aacttaacga tctggaaatg
    20401 ggcaaaattc tcggtgcctg tgaactcacc gtcggtacgc gcctgcactc tgccattatc
    20461 tcgatgaatt ttgctactcc ggcaattgcc atcaactacg aacataaatc cgccgggatt
    20521 atgcagcagc tgggactacc ggagatggca attgatatcc gtcatttatt agacggcagc
    20581 ctgcaagcga tggttgcgga taccttaggc cagcttccgg cgctgaatgc gcgacttagt
    20641 gaagccgtca gtcgtgagcg tcagacagga atgcagatgg tgcagtccgt actggaacgc
    20701 atcggggagg tgaaatgaag gtcggcttct tcttattgaa atttccgctg tcgtcggaaa
    20761 ccttcgtcct caaccaaatc actgcgttta ttgatatggg ctttgaggtg gagattgtcg
    20821 cgctgcaaaa aggcgacaca caaaacaccc acgcggcatg gacgaaatac aatcttgccg
    20881 ccagaacccg ctggttacag gacgaaccta cgggcaaagt ggcgaaactg cgccaccgag
    20941 ccagtcagac cttgcgcggc attcatcgta aaaatacctg gcaggcgctc aacctcaaac
    21001 gctatggtgc cgagtcgcgg aacctgattt tgtctgccat ttgcggccag gtcgcaacac
    21061 cattttatgc cgatgtcttt atcgctcatt ttggccctgc gggggtaacc gcagcaaaac
    21121 tacgcgaact gggtgtcatt cgcggcaaaa ttgccactat cttccacggt attgatatct
    21181 ccagtcggga agtgctcaac cactacactc ccgaatatca acaactgttt cgccgtggcg
    21241 acctgatgtt accgataagc gatttgtggg ccggaaggct gcaaaaaatg ggctgcccga
    21301 gggaaaaaat cgccgtatcg cgtatgggcg tggacatgac gcgctttagc ccgcgtccgg
    21361 tgaaagcgcc cgcaacgccg ctggaaatca tctccgtcgc acgcttaacc gaaaaaaaag
    21421 gcctgcatgt ggcgatcgaa gcctgccgtc agttgaaaga gcagggcatg gcatttcgct
    21481 atcgcatcct cggcattggc ccgtgggaac gacgcctgcg taccctcatc gaacaatatc
    21541 aactggaaga tgtggtagag atgccgggct ttaaaccgag ccacgaagtg aaagcgatgc
    21601 tcgacgacgc ggatgtcttc ctgttgccat cggtaacggg cgcggatggc gatatggaag
    21661 gcattccggt agcgctgatg gaagcgatgg cggtcggcat tccggtggtt tctactctgc
    21721 atagcggaat accggaactg gtggaggctg acaaatccgg ctggctggtg cctgagaacg
    21781 atgctcgcgc actggcgcaa cgactggcgg cgtttagcca actggacacc gacgaactgg
    21841 ctccggttgt caaacgcgcg cgcgaaaaag tcgaacacga ttttaaccag caggtgatta
    21901 accgagaact cgccagcttg ttgcaggctt tatagaggag aacgtatgcc atttaaaaaa
    21961 atctcccgac gcaccttcct gacggcaagc tccgcgcttg ccttcctcca tacccctttc
    22021 gcccgcgcac ttcccgcccg acaaagcgtt aacattaacg actacaaccc acacgactgg
    22081 atcgcctcat ttaaacaagc cttcggcgaa gggcaaacgg tcgtcgtgcc tgccggattg
    22141 gtttgcgaca atatcaacac cggcatcttt atccctcccg gtaaaacgtt acacatcctg
    22201 ggaagcctgc gcggtaacgg cagagggcgt tttgtcttac aggacggcag ccaggcgaca
    22261 ggggaggagg gcggcagtat gcataacatc accctggatg tgcgtggctc tgactgcacc
    22321 atcaaagggc tggcgatgag cggctttggc ccggtaacgc agatttatat cggcggcaaa
    22381 aacaaacggg tcatgcgcaa cctgaccatc gataacctga ccgttagcca cgctaattac
    22441 gccatcttac ggcagggatt tcataaccag attatcggtg ccaacatcac caattgtaag
    22501 ttcagtgact tacagggcga cgccattgaa tggaacgtgg caattaacga cagtgatatt
    22561 ttgatctccg accatgtcat cgagcgcatc aactgtacta acggcaaaat caactggggc
    22621 atcggcatag gccttgcggg aagtacctac gataacaact acccggaaga ccaggcagtg
    22681 aaaaactttg tcgtggcaaa tatcacgggg acggattgtc ggcagttgat ccatgttgaa
    22741 aatggtaaac attttgttat tcgtaatatc aaagcccgca atatcacgcc ggatttcagt
    22801 aagaaagcag gtattgataa cgcgacggtc gctatttacg gttgtgacaa tttcgtgatt
    22861 gataatattg aaatgattaa tagcgccggg atgttaatcg gctatggtgt aattaaaggc
    22921 aaatatctct cgataccgca aaatttccga gtgaataata ttcaactgga taatacccac
    22981 cttgcttata aattgcgcgg catccaaatc tccgccggga atgctgtctc ctttgtggcg
    23041 ctgactaaca ttgagatgaa gcgtgcatcg ctggagttac acaacaaacc gcaacatctt
    23101 tttatgcgta atatcaaagt gatgcaggaa tcctcaattg gaccagcatt gagcatgaac
    23161 ttcgacatgc gcaaagacat tcgcggcgtc tttatggcga aaaaagaaac actgctgtca
    23221 cttgcaaatg ttcatgcggt gaatgaaaaa gggcaaagct ccgtcgatat cgacaggatt
    23281 aatcaccata ttgttaatgt ggaaaaaatt aactttagat tgccggaacg gagggagtag
    23341 atttgcgacc attcttggaa aaatggagcc atacttagga acaatgctac tgcaatccac
    23401 aacgaagcgg cgtaacatca caagtaattc agtaatcaat tcagggtaat tgatgctggc
    23461 gaaaaaaatc gaacaagcta taattcagca accattttac aggtggatga aataatgacg
    23521 aatttaaaag cagttattcc tgtagcgggt cttgggatgc atatgttgcc tgccaccaag
    23581 gcgattccca aagagatgct accgatcgtc gacaagccaa tgattcagta cattgttgac
    23641 gagattgtgg ctgcagggat caaagaaatc ctcctggtaa ctcacgcgtc caagaacgcg
    23701 gtcgaaaacc acttcgacac ctcttatgaa ttagaatctc tccttgagca gcgcgtgaag
    23761 cgtcaactgc tggcggaagt gcagtccatc tgtccaccgg gcgtgaccat tatgaacgtg
    23821 cgtcagggcg aacctttagg tttgggccac tccattttat gtgcacgacc tgctattggt
    23881 gacaacccat ttgtcgtggt actgccagac gttgttattg atgacgccag cgccgacccg
    23941 ctgcgttaca accttgctgc catgattgcg cgcttcaacg aaacgggccg cagccaggtg
    24001 ctggcaaaac gtatgccggg tgacctctct gaatactctg tcatccagac caaagagccg
    24061 ctggaccgcg aaggtaaagt cagccgcatt gttgaattca tcgaaaaacc ggatcagccg
    24121 cagacgctgg actcagacat catggccgtt ggtcgctatg tgctttctgc cgatatttgg
    24181 ccggaactag aacgcactca accaggtgca tggggacgta ttcagctgac tgatgctatt
    24241 gctgaactgg caaaaaaaca gtctgttgat gcaatgctga tgactggtga cagctacgac
    24301 tgcggtaaaa aaatgggcta tatgcaggcg ttcgtgaagt atggattacg caacctcaaa
    24361 gaaggggcga agttccgtaa aggcattgag aagctgttaa gcgaataatg aaaatctgac
    24421 cggatgtaac ggttgataac aaaattataa cggcagtgaa gattcgtgga gaaagtaatt
    24481 tgttgcgaat attcctgccg ttgttttata taaacaatca gaataacaac gagttagcaa
    24541 taggatttta gtcaaagttt tccaagattt tccttgtttc cagagcggat tggtaagaca
    24601 attagcgttt gaatttttcg ggtttagcgc gagtgggtaa cgctcgtcac atcgtaggca
    24661 tgcatgcagt gctctggtag ctgtaaagcc aggggcggta gcatgggtat tttgaagcat
    24721 ttatacctcc ttattcagtg agaagggaca gattacgtaa cactgctttg ttgtactaaa
    24781 taattcgcat tttatgttca acaatcgaga tattccttat tacctaaaac tgtttttcat
    24841 tgtttataca tgatcgaata ctccttacat aaataaggag aacaaaatgg aacttaaaaa
    24901 attgatggaa catatttcta ttatccccga
//
LOCUS       DAPHDW010000060        24834 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.060, whole genome shotgun sequence.
ACCESSION   DAPHDW010000060 DAPHDW010000000
VERSION     DAPHDW010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 24834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24834
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.060"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..227
                     /locus_tag="RH057_002410"
     CDS             <1..227
                     /locus_tag="RH057_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128767.1"
                     /translation="ACNVFAFSISGGVSERSYKETEKTSAMTTTHSTKLQPSQAILFK
                     MREDAPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(304..546)
                     /locus_tag="RH057_002411"
     CDS             complement(304..546)
                     /locus_tag="RH057_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HDV3128768.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            899..1819
                     /gene="lpxP"
                     /locus_tag="RH057_002412"
     CDS             899..1819
                     /gene="lpxP"
                     /locus_tag="RH057_002412"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HDV3128769.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            2175..2246
                     /gene="ypdK"
                     /locus_tag="RH057_002413"
     CDS             2175..2246
                     /gene="ypdK"
                     /locus_tag="RH057_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HDV3128770.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(2311..3549)
                     /gene="alaC"
                     /locus_tag="RH057_002414"
     CDS             complement(2311..3549)
                     /gene="alaC"
                     /locus_tag="RH057_002414"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HDV3128771.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKRYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENRDRIRQAIRGIKA
                     MFRADGLLPASSKHIHENAE"
     gene            3926..5623
                     /gene="ypdA"
                     /locus_tag="RH057_002415"
     CDS             3926..5623
                     /gene="ypdA"
                     /locus_tag="RH057_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     YpdA"
                     /protein_id="HDV3128772.1"
                     /translation="MHEIFNMLLAVFDRAALMLICLFFLIRIRLFRELLHKSAHSPKE
                     LLAVTAIFSLFALFSTWSGVPVEGSLVNVRIIAVMSGGILFGPWVGIITGVIAGIHRY
                     LIDIGGVTAIPCFITSILAGCISGWINLKIPKAQRWRVGILGGMLCETLTMILVIVWA
                     PTTALGIDIVSKIGIPMILGSVCIGFIVLLVQSVEGEKEASAARQAKLALDIANKTLP
                     LFRHVNSESLRKVCEIIRDDIHADAVAITNTDHVLAYVGVGEHNYQNGDDFISPTTRQ
                     AMNYGKIIIKNNDEAHRTPEIHSMLVIPLWEKGVVTGTLKIYYCHAHQITSSLQEMAV
                     GLSQIISTQLEVSRAEQLREMANKAELRALQSKINPHFLFNALNAISSSIRLNPDTAR
                     QLIFNLSRYLRYNIELKDDEQIDIKKELYQIKDYIAIEQARFGDKLTVIYDIDEEVNC
                     CIPSLLIQPLVENAIVHGIQPCKGKGVVTISVAECGNRVRIAVRDTGHGIDPKVIERV
                     EANEMPGNKIGLLNVHHRVKLLYGEGLHIRRLEPGTEIAFYIPNQRTPVASQATLLL"
     gene            5638..6372
                     /gene="ypdB"
                     /locus_tag="RH057_002416"
     CDS             5638..6372
                     /gene="ypdB"
                     /locus_tag="RH057_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HDV3128773.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            6385..7242
                     /locus_tag="RH057_002417"
     CDS             6385..7242
                     /locus_tag="RH057_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3128774.1"
                     /translation="MKAPGLPADQQFFADLFSGLVLNPQLLGRVWFASQPASLPVGSL
                     CIDFPRLDIVLRGEYGNLLEAKQQRMVEGEMLFIPARAANLPINNKPVMLLSLVFAPT
                     WLGLSFYDSRTTSLLHPARQIQLPSLQRGEGEAMLTALTHLSRSPLEQNIIQPLVLSL
                     LHLCRNVVNMPPGNSQPRGDFLYHSICNWVQDNYAQPLTRESVAQFFNITPNHLSKLF
                     AQHGTMRFIEYVRWVRMAKARMILQKYHLSIHEVAQRCGFPDSDYFCRVFRRQFGLTP
                     GEYSARFQG"
     gene            complement(7245..9740)
                     /gene="ptsP"
                     /locus_tag="RH057_002418"
     CDS             complement(7245..9740)
                     /gene="ptsP"
                     /locus_tag="RH057_002418"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HDV3128775.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFFNHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQVRRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPIDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(9765..10802)
                     /gene="ypdE"
                     /locus_tag="RH057_002419"
     CDS             complement(9765..10802)
                     /gene="ypdE"
                     /locus_tag="RH057_002419"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3128776.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEADRLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARSYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLGCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTVVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(10802..11887)
                     /gene="ypdF"
                     /locus_tag="RH057_002420"
     CDS             complement(10802..11887)
                     /gene="ypdF"
                     /locus_tag="RH057_002420"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3128777.1"
                     /translation="MTLLASLRDWLKAQQLDAVLLSSRQNKQPHLGISTGSGYVVISR
                     ESAHILVDSRYYVEVEARAQGYQLHLLDATNTLTTIVNQIIADEQLQTLGFEGQQVSW
                     ETAHRWKSELNAKLVSATPDVLRQIKTPEEVEIIRLACGIADRGAEHIRRFIQAGMSE
                     REIAAELEWFMRQQGAEKASFDTIVASGWRGALPHGKASDKIVAAGEFVTLDFGALYQ
                     GYCSDMTRTLLVNGEGVSAESHPLFDVYQIVLQAQLAAISAIRPGVRCQQVDDAARQV
                     ITEAGFSHYFGHNTGHAIGIEVHEDPRFSPRDTTTLQPGMLLTVEPGIYLPGQGGVRI
                     EDVVLVTPQGAEVLYAMPKTVLLTGEA"
     gene            complement(11902..13149)
                     /locus_tag="RH057_002421"
     CDS             complement(11902..13149)
                     /locus_tag="RH057_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HDV3128778.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAVI
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(13171..13497)
                     /locus_tag="RH057_002422"
     CDS             complement(13171..13497)
                     /locus_tag="RH057_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HDV3128779.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQALEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAVGIIKEIEEM
                     IASEQQ"
     gene            complement(13716..14681)
                     /gene="glk"
                     /locus_tag="RH057_002423"
     CDS             complement(13716..14681)
                     /gene="glk"
                     /locus_tag="RH057_002423"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HDV3128780.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDNPGLLGSGAHLRQTLGHIL"
     gene            14885..16141
                     /locus_tag="RH057_002424"
     CDS             14885..16141
                     /locus_tag="RH057_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HDV3128781.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASALQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPG
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            16256..16582
                     /locus_tag="RH057_002425"
     CDS             16256..16582
                     /locus_tag="RH057_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV3128782.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(16723..17961)
                     /locus_tag="RH057_002426"
     CDS             complement(16723..17961)
                     /locus_tag="RH057_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HDV3128783.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLAMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            18297..19499
                     /gene="nupC"
                     /locus_tag="RH057_002427"
     CDS             18297..19499
                     /gene="nupC"
                     /locus_tag="RH057_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HDV3128784.1"
                     /translation="MDRVLHFVLALAVVAILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(19549..21738)
                     /locus_tag="RH057_002428"
     CDS             complement(19549..21738)
                     /locus_tag="RH057_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
                     /protein_id="HDV3128785.1"
                     /translation="MFVEHNLIKNIKIFTLAFTLTVVLIQLSRFISPLAIIHSSYIFL
                     AWMPLCVMLSILFIFGWRGVVPVLCGMFCTNLWNFHLSFLQTAVMLGSQTFVVLCACA
                     ILRWQLGTRWRYGLTSRYVWQRLFWLGLVTPIGIKCSMYLVGSFFDFPLKISTFFGDA
                     DAIFTVVDLLSLFTAVLIYNMLFYYLTRMIVSPHFAQILWRRDIAPSLGKEKRAFTLS
                     WLAALSVLLLLLCTPYENDFIAGYLVPVFFIIFTLGVGKLRYPFLNLTWAVSTLCLLN
                     YNQNFLQGVETEYSLAFILAVLISFSVCLLYMVRIYHRSEWLNRRWHLQALTDPLTLL
                     PNFRALEQAPEQEAGKSFCCLRIDNLEFMSRHYGLMMRVHCIRSICRTLLPLMQENEK
                     LYQLPGSELLLVLSGPETEGRLQHMVNILNSRQIHWNNTGLDMGYGAAWGRFDGNQET
                     LQPLLGQLSWLAEQSCAHHHVLALDSREEMVSGQTTKQVLLLNTIRTALDQGDLLLYA
                     QPIRNKEGEGYDEILARLKYDGGIMTPDKFLPLIAQFNLSARFDLQVLESLLKWLATH
                     PCDKKGPRFSVNLMPLTLLQKNIAGRIIRLFKRYHISPQAVILEITEEQAFSNAESSM
                     YNIEQLHKFGFRIAIDDFGTGYANYERLKRLQADIIKIDGVFVKDIVTNTLDAMIVRS
                     ITDLAKAKSLSVVAEFVETQQQQALLHKLGVQYLQGYLIGRPQPLAD"
     gene            complement(21946..22021)
                     /locus_tag="RH057_002429"
     tRNA            complement(21946..22021)
                     /locus_tag="RH057_002429"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(21986..21988),aa:Ala,seq:ggc)
     gene            complement(22061..22136)
                     /locus_tag="RH057_002430"
     tRNA            complement(22061..22136)
                     /locus_tag="RH057_002430"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22101..22103),aa:Ala,seq:ggc)
     gene            22357..22716
                     /locus_tag="RH057_002431"
     CDS             22357..22716
                     /locus_tag="RH057_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV3128786.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            22718..23110
                     /locus_tag="RH057_002432"
     CDS             22718..23110
                     /locus_tag="RH057_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HDV3128787.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWIREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(23162..24577)
                     /gene="gltX"
                     /locus_tag="RH057_002433"
     CDS             complement(23162..24577)
                     /gene="gltX"
                     /locus_tag="RH057_002433"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HDV3128788.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
BASE COUNT         6197 a         6585 c         6100 g         5952 t
ORIGIN      
        1 tggcatgcaa tgtttttgca ttttccattt ctggtggtgt aagtgagagg agctataaag
       61 agactgaaaa aacatcagcg atgacaacca cacactctac aaaacttcag ccatcacagg
      121 cgattttgtt taagatgaga gaagatgcgc caccattaaa cctcacagaa gaaatcacgc
      181 ctacttatcc gacaaaggcg aattatctta ttcatcctgt gcgatagcgt ctgtcacatg
      241 gcaggtgaac aatcatgtct gatgctgatg tgaacacctg cccaaatgag ccatcctgac
      301 ccactatcgc ctgccatacc ccaccataaa gcgaatcatc aagccacaag ccggggcaag
      361 aaacataaat atccccgtca ctagttcatt accgccagtt aaccacatgg tgaagtaata
      421 aatgagtgca gcgaggatca ccatgccgaa gaaagaagag accgccgata ccacgttcag
      481 tacctgaccg atgtagccgc ttacgcagac aatacacagg gcgagaaaca tccataaata
      541 aatcatagct caacctcctg ccgcgctaaa aaacctgtaa cttcagtcac tttaatagta
      601 agacaatcct taacctctgg caatttttgc taagcatcaa tgaaaacagc atgttaaatg
      661 caagactgtt gtgtacggaa aaatatttac tttgcacgat taataatcag tagctgaaag
      721 cagtcagcga gtgataaagt acagataccg cattcatgct cctcgtggtt atgtcctgac
      781 tagtctttac actctttaca ggaaccattg tcgtacatga tggcccaacc aattgagtgt
      841 accgctgtcg acatggaatc ttctgcgata caacaattcg tatctacaga aggtaactat
      901 gtttccacaa tgcaaatttt cccgcgagtt tctacatcct cgctactggc tcacatggtt
      961 tgggcttggt gtactctggc tttgggtaca gcttccttat cctgttctct gctttctcgg
     1021 cacgcgcatt ggcgcaatgg cgcgaccatt cctgaaacgt cgtgaatcta tcgcccgtaa
     1081 aaacctggaa ctttgtttcc cgcagcattc tgcggaagaa cgcgagaaga tgattgccga
     1141 aaactttcgt tcactcggca tggcgctggt agaaaccggc atggcatggt tctggcccga
     1201 cagtcgcgta cgtaaatggt ttgatgttga agggttggat aaccttaaac gcgcacaaat
     1261 gcaaaatcgc ggcgtaatgg ttgtcggcgt ccattttatg tcgctggaac tgggcggccg
     1321 cgtgatggga ctgtgccaac caatgatggc tacctatcgt ccacataata atcagctgat
     1381 ggaatgggtg cagacccgtg ggcgcatgcg ctctaacaaa gcgatgatcg gcagaaataa
     1441 tctgcgcggc attgtcggtg cactgaagaa aggtgaagcg gtatggtttg ctcccgatca
     1501 ggattatggt cgtaaaggca gctccttcgc gccgttcttt gcggtggaaa atgtcgccac
     1561 aaccaatggc acctatgttc tctcccgtct ctctggcgca gccatgttga ccgtaacgat
     1621 ggtaagaaaa gcggattaca gcggatatcg tttgttcatc accccagaga tggaaggcta
     1681 cccgacagat gaaaatcaag ccgctgccta tatgaacaag attatcgaga aagagatcat
     1741 gcgcgcaccg gagcagtacc tctggatcca ccgtcgcttt aaaacgcgcc cggtgggaga
     1801 atcgtcgttg tacatttaac aaactgctct tactctttcc ctcctcaaat cggccataat
     1861 aacctcatgg tttttatggc ttttttctta aggttttttc cttcgctgaa tatctaatta
     1921 ttaccgtcag aaattaaact gtcgccgata tgagacactt gtaagttact aaaagtaaat
     1981 aagaaaatgc ctcttgtctc gtttcagttt taggcgtaca ttagcgccgt ctcgagcagt
     2041 ttagctcaga attctggggt gatgcccacg gtaaaataca gggcttttaa ggtcgctttg
     2101 cccggtgtca actcactgta tcaggtgtaa tgaagtcatt caggcgtaac agtaattacg
     2161 cggagagatg taaagtgaaa tatttcttta tgggcatttc ttttatggtc atcgtttggg
     2221 ccggtacttt tgccctgatg atctaaaagc aaactgtgca atgaaaaaca ggagccatca
     2281 ggctcctgtt tgcatttctg acgacggtgt ttattccgcg ttttcgtgaa tatgtttgct
     2341 gctggcgggt agtaaaccgt cggcacggaa catcgcttta atcccacgaa tcgcctgacg
     2401 aatacggtcg cggttttcaa tcagtgcaaa gcgaacatgc gtatcaccat agtcgccaaa
     2461 gccaatcccc ggcgagacac aaaccttcgc ttcgttaagg agcttcttgg caaattccag
     2521 cgatcccatg gccgcatatg gttccgggat tttcgcccag acatacatcg aagccttcgg
     2581 catttcaacc atccagcccg cttcatgcag ccctttaacc aatacatcac ggcggcgttt
     2641 gtactgttcg gcaatgtcgc gcacgcactg ttgatcgccc tccagcgccg caatcgccgc
     2701 cacctgcaac ggcgtaaagg tgccgtaatc gtgatagctt ttaatacgag ccagggcgct
     2761 gaccagcgtt ttgttgccaa ccataaagcc gatacgccag cccgccatgt tgtagctttt
     2821 cgacagcgta aagaactcga ctgccacatc gcgtgcaccc ggtacctgca tgattgacgg
     2881 cgctttccag ccatcgtaga cgatatcggc ataggccagg tcgtggacca ccagcacatc
     2941 gtagcgtttc gccagcgcca caaccttttc gaagaactcc agctccacgc attgcgcggt
     3001 tggattcgac gggaagccga ggatcatcat cttcggtttc ggataacttt cacgaatggc
     3061 gcgctccagt tcgttgaaga aatcgacacc ttccaccagc ggcactgagc gtacctgcgc
     3121 cccggcaatc accgcgccat aaatatggat cgggtaactt ggattcggca ccagcaccgt
     3181 gtcaccatga tccagcgtcg ccagcatcag atgcgccagg ccctctttcg aaccaatagt
     3241 gacgatggct tctgattccg ggtcgatttc aacgtcgtag cgatcctgat accagcggga
     3301 aatggcgcga cgtaaccgcg gaatgccgcg tgaagtggag taaccatgcg tgtccgggcg
     3361 ctgggccaca gtacataatt tttcgacgat atgcggcgga gtcgcaccgt ccgggttacc
     3421 catgctgaaa tcgataatat cttcgccgcg ccgacgcgca gccattttca gttcagcggt
     3481 gatgttaaaa acgtagggcg ggagacgatc aatgcgcgta aagcgacgtt cagggcgagt
     3541 gtcagccata atttcctcag attaacgtta gcgcccggac cgtccgagcg acgctgccac
     3601 aatggtggct gttttgaaaa tagcctgatt aatttctgtc tgtccagcgt tttagaacat
     3661 ttttatcaat ggctttaaat gaggaactcc acttcaaaaa caggaaattt ttctacgcta
     3721 acaagaaaat taccaccaat tttattaaca ttataatttc aataaattta ccagccagaa
     3781 gtgcattatc aagagataca ttgctaatac accatttatc tttcaatccc cttttaaatt
     3841 tatggttttt cctcaaaaac ggcctgctgg tcatcagcct tcaggttacc tatcatagag
     3901 gtttaatcct tattcagagt cacccgtgca cgaaatattc aacatgctgc tggcggtctt
     3961 cgatcgggca gcgttaatgc ttatctgcct gttctttctc atccgtatcc gcctgtttcg
     4021 cgaactgttg cacaagtcgg cgcactcccc aaaagaattg ctcgccgtta ccgccatttt
     4081 ctcgctgttc gccctgttca gcacctggtc cggcgttccc gtagaaggct cgctggtgaa
     4141 cgtacgtatt atcgcggtga tgtccggcgg gattctgttt ggcccgtggg taggcatcat
     4201 taccggcgtg attgcgggta ttcaccggta tttaattgat atcggcggcg tgacggcgat
     4261 cccctgcttt atcaccagca ttctggcggg ttgtatatca ggctggataa acctgaaaat
     4321 ccccaaagca cagcgctggc gcgtcggtat tctcggcggc atgttgtgtg agacgttgac
     4381 catgattctg gtaattgtct gggcaccaac taccgcgttg gggatcgata tcgtctctaa
     4441 aatcggcatt ccaatgatcc tcggtagcgt ctgtatcggc tttattgtgc ttctggtgca
     4501 aagcgttgag ggcgaaaaag aggccagcgc cgcgcggcag gccaagctgg cgctggatat
     4561 cgccaacaaa acgctacctc tgtttcgcca tgtcaatagc gagtcattac gcaaagtttg
     4621 tgaaattatt cgcgatgaca ttcacgccga tgcggtggcg attaccaata ccgatcatgt
     4681 gctggcctat gttggcgtgg gtgaacataa ctatcagaat ggcgatgact tcattagccc
     4741 gactacccgt caggcgatga attacggaaa aatcatcatt aaaaacaatg atgaagccca
     4801 ccgcacacca gagattcatt ccatgctggt gatcccattg tgggagaaag gcgttgtgac
     4861 cggaacgctg aaaatttact actgccacgc gcatcagatc acctcgtcat tacaggaaat
     4921 ggcggtcggt ctgtcgcaaa tcatctccac ccaactggag gtttcacgcg ccgagcagct
     4981 acgtgaaatg gcaaataagg cagagcttcg cgccctgcaa agcaaaatta atccccattt
     5041 cctgtttaac gctttgaacg ctatttcatc atcaatccgt ctgaatccgg atactgctcg
     5101 ccagctgatc tttaatctgt cgcgttatct gcgctataac attgaattaa aagacgatga
     5161 gcaaatcgat atcaaaaaag agctgtatca aattaaagac tatattgcca ttgagcaggc
     5221 ccgctttggt gacaagctga cggttatcta tgatattgat gaagaggtga attgctgcat
     5281 tcccagcctg ctgatccagc cgttggtgga gaacgccatt gtccacggta ttcagccttg
     5341 caaaggtaaa ggcgttgtca ccatcagcgt tgcagagtgc ggaaatcggg tccgcattgc
     5401 ggtgcgagat accgggcacg gcatcgatcc aaaggtgatt gagcgggtcg aagcgaatga
     5461 aatgccgggc aataaaattg gcctgctgaa tgtccatcat cgcgtgaagt tattgtatgg
     5521 cgaggggctg catatccgcc gcctggagcc ggggacggaa attgcgtttt acattcctaa
     5581 ccaacgcacc ccagtcgcct cacaggctac gttattgctt tgagccggag tgatattgtg
     5641 aaagtcatca ttgttgaaga cgaattcctg gcacaacagg aactgagctg gctaattaaa
     5701 gagcacagcc agatggagat tgtcggcacc tttgacgacg gtctggacgt gttgaagttt
     5761 ttgcagcata accgcgtcga cgccattttt ctggatatca atattccgtc gctggatggc
     5821 gtgttgctgg cgcaaaacat cagccagttc gcccataaac cgtttattgt gttcatcacc
     5881 gcgtggaaag aacatgcggt agaagcgttt gaactggagg cgtttgacta cattctcaaa
     5941 ccgtatcagg agtcacgtat taccgggatg ctgcaaaaac tggaagcggc ctggcaacaa
     6001 cagcagacca gcagtacgcc tgccgcgacg gtaacgcgtg agaatgacac cattaatctg
     6061 gtgaaagatg agcgaataat cgtcacgcca attaacgata tctattacgc cgaagcgcac
     6121 gagaaaatga cctttgtcta tacgcggcgt gaatcctacg taatgccgat gaacattacc
     6181 gaattttgca gcaaactgcc gccgtcgcat tttttccgct gccatcgctc attttgtgtc
     6241 aatctgaaca aaatacgcga aatcgaaccg tggtttaata acacctacat tctgcgactg
     6301 aaagatctgg attttgaagt gccggtcagc cgcagcaaag tgaaagaatt tcgccagtta
     6361 atgcatcttt aatcgggaac tttcatgaaa gcaccaggct tgcccgccga tcaacaattt
     6421 ttcgccgatc tgttcagcgg cctggtgctt aacccgcaac tactggggcg cgtctggttt
     6481 gccagccagc ctgcctcgtt accggtgggc agtttatgta ttgattttcc ccgtctggat
     6541 atcgtgctgc gcggcgaata cggcaacctg ctggaagcaa agcagcagcg aatggtggaa
     6601 ggagaaatgc tgtttattcc ggcacgcgcg gctaatttac cgatcaacaa caaaccggtg
     6661 atgctgttaa gcctggtgtt cgcaccgacc tggcttgggt tatcgtttta cgatagccgc
     6721 accacgtcgt tgttgcatcc tgctcgccag atccagcttc ccagcctgca acgcggtgaa
     6781 ggtgaagcga tgcttaccgc cctcacccat cttagccgtt cgccgctgga gcagaatatc
     6841 attcagccac tggtgttaag tttgctgcat ctttgccgca acgtggtgaa tatgccgccg
     6901 ggtaattcgc aaccgcgcgg cgattttctc tatcacagca tttgtaactg ggttcaggat
     6961 aattatgccc agccgctcac ccgcgagagc gtggcgcagt tttttaatat cacgcccaat
     7021 catctgtcaa aactgtttgc tcagcatgga acgatgcgtt ttatcgagta tgtgcgttgg
     7081 gtgcgaatgg cgaaggcgag gatgattttg cagaaatatc atctgtcaat tcatgaagtg
     7141 gcacagcgtt gcggttttcc ggatagcgac tatttttgtc gcgttttccg gcgtcagttt
     7201 ggtctgacgc cgggagagta cagcgcccgt tttcagggct aacgtcagaa ggttaattct
     7261 gtttccagca gcgtcaggat actttgtgca tcttgcgcgg caaacaacga ctggcggaag
     7321 tttttattca ccagtttacg cgccagctgc gagaagactt tcacatgatt catcccttcg
     7381 ttagcaccca gcgtcagcat gatcaccagt tcgacttcgc ccatttctga ctgccagtca
     7441 atcggtttcg ccagccgggc aatgctgata ctggaatgac ggatccactg agatttggtg
     7501 tgcgggatcg ctacgccaaa accaacgccg gtggtaacaa tctcttcccg ctgccagaca
     7561 tcttcttcca gctcaaacgg atgttcagtg cgcccgttaa cgccgaggtt gccgcacagg
     7621 aactggatcg cctgctcttt attgctaaaa tcctgatcaa caaagatatt ctccagcgcc
     7681 agcagtgggc gaacgtcttc ttccggcgta aaggcggtga gtaacgcttc aatttcctgc
     7741 gcactgcggc attcacatgc ctgacgcgcc agttcccgac acgcctcgct atccagttga
     7801 cgaagctggc ttttcaccgc cggaatacgc gggctactca tactcagctc atccaggccc
     7861 agcccaagca gtagcggcag ataacggctt tcaccgccca gttcaccgca aatgcctacc
     7921 catttgcccc gctgatgcgc agtggtaaca atctgctgca acatgcgcag gaacgatggc
     7981 gtaatcgggt tatacagcgg cgatacgcgc gggttattac gatcgaccgc atacagatac
     8041 tgggtcatat cgttagaacc gatactgaag aaatcgacct catcgcagaa gtggtcgatg
     8101 atgtaacaca ccgacggcac ttccaccatg atacccagag taatcgtctc tgcatgacgc
     8161 aggccatcgc gcttaagctc aacgatcgct ttttggatct cgcccttcac ccataagatc
     8221 tgatcgaggc tgtgaaccat cgggatcatc aactgggcgt tgccaaaact ggcagcgcgc
     8281 aaaatcgccc gtagttgagt gcggaacagg ccagcaaatt ccgggtaaat acgtaccgcg
     8341 cgatagccga ggaacgggtt ctcttcctgg ggaatattca gataaggaat gcttttatcg
     8401 cctccgatgt ccattgtgcg gaagataatc ggcttgtcgc ccgctgccag tagcacctgc
     8461 tggtaggctt caaattgctc ctgctcgtcc ggcgcgctgt cgcgatccat atagagcatt
     8521 tcagtacgga acagtccaac gccttccgcg ccgttggcaa acgcgcctgg cgcttccaga
     8581 gcggtgccga tattcgccgc aatatcaata cgcttgttat cacgggaata ggccagctgc
     8641 gcggcggcct gcgcctgttg tttttggcgt ttatccgcca gcgtctgtgc gacctgataa
     8701 taaccgctca ccgcgtcatt cgggttaatc gccagcacgc cgcactgggc gtcaagcacc
     8761 gccggttgcc cggcataacg ggcaatcgca tccagcggca ggccgctcag taccgggatc
     8821 gccgaggcgc gggccagaat cagggtatgc gaggtgcgcc cagttttctc cagaatcatg
     8881 cctgcaagat tttttaaatc gaggctcaaa aactggcttg gggttaaatc ctcggctacc
     8941 agaatagtcg gtttttccag caccagattg ttgcgcggct tcagttccgg ccaggtgata
     9001 tgcagcaact gttcgctaat atcgcgaatg tcgctaacac gttcgcgcag ataatcactg
     9061 gcagaagcag aaagtttggc gcaaacctgc tccatattgc tgatgatcgc cgcccccagc
     9121 ccttgatgct gttctgtcat cagacgacgg atattgcccg cgaattcatc atcctgaatc
     9181 agcgacaaat gggcgctgag gatagttttg ctttcgccgt cacgctcacg caattgctgg
     9241 ttcagttgct cggcaagcgt tgccaggctg tgctccagcc gggtggaatc ttgcgcactg
     9301 gcggggattg cccgataact gtcgaggctg tcgctttgta acagggtcag cgtacccacg
     9361 ccgacgccgc ttgccagcac attgccgtac agtaaatccg ggttaaggcg gcttaatgaa
     9421 cgcggcagcg gatgcgccgt cagttctgcc tgcgtcggct gaacgctgtc gctgtcgata
     9481 aagcgcacca ggatgtactc ttccagcacg cgccgcacct gctcttcatc gctgccgcta
     9541 atgttcaggc tgcaactgtc attaaataag gtgccagttc caatcaacgc cagcgagctt
     9601 ttagcatcgg cctttgcgtt ctggcgatgg ttgaaaaagg tgatttcgct ttgccactgg
     9661 ctgcactgtt ctttaagttc ccacgccgga cgagcatgta gaccgttagg caaaggacag
     9721 agaaattgaa tcgttaacat aaccgctccg ggttaatcag gagatcatct gaaatccgtc
     9781 agttgaacaa ccgtctcacg cgtaagacgt tgaataaggg cagataaaag ttgctccatc
     9841 tgcaaaatgt cgcggcaatc ggcaatcgat gccgcgcaat gtccgtggcg ggtggcaggc
     9901 cccattacca ctgtgggtac gccagtaccg gttaagtgca ccgctccgcc gtccgtgccg
     9961 ccgttgctga acatatctgc ctgcaacggt acgccaattt cagccgccac ggtttcgatc
    10021 caggcggtaa gttttggcgg cgcaatcagt gacttgtcgc ttaacaccag catcgggccg
    10081 ttaccaatct ggcgatggtt agccgcgcca taatcaaagt ttttcgccca gcaggcggta
    10141 tccagcacaa tggcgacgtc cggcgacacc gcgcgggtag cggtttgccc accgcgtaat
    10201 cccacctctt cgctggaact tgccaccagc cacacctccg caggcagttc ggcatcgtgc
    10261 agctcacgta gtaacgtcac cagcagatag caaccgaggc gatcatcaaa ggctttcccc
    10321 atcacccgct ggtgagggag aacctgaaaa gttgtatcaa aggtgacgcg atcgcctgga
    10381 cgaattcccg cctgcatcac ttcgtcatac gagcgcgcac caatgtccac gcgcatggcg
    10441 ctgacgtcat tcccctgccg gtcgccgtca agcaggcctg gaattttgca ctcttcacgg
    10501 gtggtgatgc gcaccggctg cagctggcgg gcagccatgc gcacgttgcc aaccggcagc
    10561 acatcaattg ccccttcgcg ggagatgcta cgcaccataa atcccacttc gtccatatgg
    10621 gcgcagatca tcaccttcgg acctgtcgat tcattgaggc ggatcagcac cgatcccaga
    10681 ccatcaaatc gcacttcttt ttgcaggcga tccgcttctt ccagcaggat ctgccgcact
    10741 tcctgttccg aggaggcgat cgcatctgcc tcgctcaacg cttttaatag cgataaatcc
    10801 attatgcctc tcccgtgagc aacactgttt tcggcatggc gtagagcact tctgcgcctt
    10861 gcggggtaac cagcacaaca tcttcgatgc gcacgccccc ttgccctggc aaataaatcc
    10921 ccggctccac ggtcagtaac atgcctggct gtagcgtcgt ggtgtcccgc ggtgaaaaac
    10981 gcggatcttc atgaacttca atgccgatag catgaccggt gttatgaccg aaatagtggc
    11041 taaaacctgc ctcggtaatc acttggcgcg cggcatcgtc aacctgctgg cagcgcacgc
    11101 cggggcgaat tgcagagatt gccgcgagct gtgcctgcag gacaatctga tagacgtcaa
    11161 acagcgggtg agattcggcg ctcacccctt cgccattcac cagcaaggtg cgcgtcatat
    11221 cagagcagta gccctgatac agcgcaccaa aatcgagagt gacaaactcg cccgctgcaa
    11281 caatcttgtc gctggctttg ccgtgcggca gcgccccgcg ccagccgctg gcgacaatgg
    11341 tgtcaaaaga ggctttttct gcgccctgct ggcgcataaa ccactccagt tcagcggcta
    11401 tctcgcgctc gctcatcccc gcctgaataa agcggcgaat atgctccgct ccgcgatcgg
    11461 caatcccaca ggcgaggcgg attatctcca cctcctctgg cgttttgatt tgccgcagca
    11521 catccggcgt ggcgctaacc agtttcgcat tgagttcaga cttccagcgg tgcgcggttt
    11581 cccaactcac ctgctggcct tcaaaaccga gcgtttgcaa ctgctcatcg gcaatgattt
    11641 gattgacgat agtggtaagc gtgttcgtcg cgtcaagcaa atgcagctgg tagccttgcg
    11701 cacgggcttc cacctcaacg taatagcgcg aatccaccag aatgtgcgca ctttcacggc
    11761 taataaccac atagcctgat ccggtggaga tccccagatg cggctgtttg ttctgccgtg
    11821 aggagagaag cactgcatcc agttgttgcg ccttaagcca gtcgcgcagc gaagcgagta
    11881 atgtcatctg gattttcctt cttacaggct atcgattaac aatttgcctt tacggaacat
    11941 catcagacgc aggaacacca ccatcagcgc agttatgact gcccccagcg cgatccccgc
    12001 catatagacg cccaggttag tgaccagcgg ccatgcccag atagcggact ccgggaacca
    12061 ttgcactgcg cccagccaga cagcggcggt tgagccgaca atagcgccga ccatatacga
    12121 cggaatggcg gtgatggggc tttccagcgc aaacggaatc gcaccctcac tgatccccat
    12181 aaacgccagg aacatggcgg tcttaccctg cggataaagt tgagcgttga acaggcgttt
    12241 gccggttaaa cgacggtcga taatggtcgc cagccccaga ccaatcggcg gaataacgat
    12301 agcgatagaa cgcgcggtaa ccggtaatac gtggtcggtg gtaaagctga aggcaacgaa
    12361 acctgccgct ttgttgattg ggccaccaag gtcgattgcg gtcgccgcgg caatccccat
    12421 tgcatacatc agcgcgccct tctcacctgc ggcagtcagc acggtacgga taccgccgtt
    12481 gatccagcca ccaaatgggg tgatgacata gtacatcgcc agcatgacaa aaatggcgga
    12541 aagaatcggt agcaaaaatg tggttttgaa cgctaacagg aaatctggta actggatttt
    12601 ctggttcatc catttcacaa ggtaaccagc aacgatagat ataatcagcg caccaatgaa
    12661 ggtggacggc accggcgaag aggtcgccca ttgcatagtg ctgggatcga agttcagcag
    12721 ttgcgtcggc tgggtagaca tcaagccgcc gataaaacca gccgggaacg ccagtttgcc
    12781 gccgatagag ttcgccacaa aagcggcaaa catcggaata gcaaagccaa acaacacgcc
    12841 gccgaaggac tgcgacagcc aggcaaattt cagtaaggag aggtcgaaac cggagaattt
    12901 cccgctatta agagcatcca tgatgccgat ctcagcagga attttaagcc agctataagc
    12961 aatcagctgg ctgaaagcga ggatcacacc gcccataatt aaggtcggca ccatgcgtga
    13021 aatccctgac atcacatgct gcgggagttc accccagaag ctgcttttag aggcctgcgg
    13081 attcttaact gccgctgccg caccggatgc gccaggcaca acggttgcac tgcgtttttt
    13141 aatggccata acaattatcc ctgtcgaata ttactgttgt tcagaggcaa tcatctcttc
    13201 gatttctttg atgatgccta cagcgttttt aattgcgtcc tgcaaagtga tttcataaac
    13261 gtcgcgtgat tcgaaacgtt cgttatcttc cggggtaact gccacggaat ggatgatgat
    13321 ggtcgcttcg gcgatatcct gcgccgtcag gcgattctgg atgccgtccg cgccctgggt
    13381 ttcaattttc acttcataac cggcttctac cgccgcttct tccagcgcct gagcggccat
    13441 aaaagtgtga gccaggccca tcgggcaggc acataaggca atcagtttct tactcatcgt
    13501 taattcctcg ttcagtgata gttgacgagt gaaattatga acgtgcaaaa caaatcgccg
    13561 ttaccagaca aataatgcat ttactggaga attaatcccg ctgtatggaa gtgtgacgca
    13621 ggtcggcctt gtgaggggac acaaagaaaa attgaattaa ctgtatgatt taaaagatta
    13681 tcgggagagt tacctcccga tataacagga aggatttaca gaatgtgacc taaggtctgg
    13741 cgtaaatgtg caccggaacc gagaagaccc ggattgtcat ggacgatgag atacaccgga
    13801 atatcatgga catattcttt aaagcgccct ttatcttcaa atgcggcacg gaaaccagag
    13861 gctttgaaga actcaaggaa gcgcggcacg ataccgcccg caataaacac gccgccaaat
    13921 gtcccgagat tgagcgccag attgccgcca aaacggccca taatgacgca aaacagcgac
    13981 aacgcgcggc ggcaatcggt gcagctatca gccagcgcac gttcggtaat atcttttggc
    14041 ttgagatttt ctggcaggcg gttgtcagct ttcacaattg cgcgatacaa attcaccagc
    14101 ccagggccag aaagcacgcg ttccgccgaa acatggccaa tttccgcacg cagtatttcg
    14161 aggataatgg cctcttcttc actattcggc gcaaaatcaa cgtgaccgcc ttcgcccggc
    14221 aagcttaccc aacgcttatc gacatggacc agatgcgcaa ccccaagccc cgttccggca
    14281 ccgtaaaccg caataggctt accttctacc ggttctgcgc caccaaactg aatcagatgc
    14341 tcttttttca gcatcgggat cgccatcgat acagcggtaa aatcgttaat aatttccaga
    14401 tggctaaaac cgagattctt tttcatttcg gcaattgaga acgcccaggt atggttggtc
    14461 attgccaccc agtcaccggt aattgggcaa gcgatggcaa tacagccgtc tttcacctcg
    14521 accttatgtt cttcaagata aacgcgaatg accgcttcga ggctggggta atcaagccct
    14581 gaataggtct tagcctgcga gatttcacca ctggcaatat cacacagagc aagacgtgcg
    14641 ttggtgccgc ccacatcacc gactaatgca tactttgtca ttcttcaact gctccgctaa
    14701 agtcaaaata attctttctc acactgtaaa tacctggggg cataacaaca acgccggaaa
    14761 ggcaggctcc ctgtaaatat cgatctgggt cacacattta ctttatcgtt tcagcaccaa
    14821 ttgcagcgat gcctttttgc aagctgggca aactaagtat ctgaccccgc ataaggaata
    14881 gaacatgctc catccgcgag ccagaaccat gttgttatta tcgctccccg ccgtggcaat
    14941 tgggattgcg tccagtctta ttctgattgt ggtgatgaaa atcgcctcgg cattacagaa
    15001 tttgctctgg caacgactgc cgggaactct ggggatagcc caggattcac ctctctggat
    15061 catcggtgta ttaacgctaa cgggtattgc ggtggggttg gttatccgtt tcagccaggg
    15121 tcatgccgga ccagaccccg cctgtgaacc gctgatcggc gcaccagttc cgccctctgc
    15181 gctaccagga cttatcgtag cattaattct cggtcttgct ggcggcgtca gcctggggcc
    15241 ggaacatccg atcatgaccg tcaatatcgc ccttgcggtt gccattggcg ctcgtctgtt
    15301 accgcgcgtc aaccgaatgg agtggactat tttagcctct gccggaacca tcggcgcgct
    15361 gtttggtaca cctgttgcgg cggcgttgat attttcgcaa accttaaatg gcagtagtga
    15421 agttccgcta tgggatcgtc tttttgcgcc attaatggcg gcagcagctg gtgcacttac
    15481 taccggatta tttttccatc cccatttttc actgcccatt gcgcattacg gacagatgga
    15541 aatgaccgat attctcagcg gtgcaattgt cgcggcgatt gccatcgcag cagggatggt
    15601 cgccgtatgg tgcttaccac ggttgcacgc gatgatgaat caaatgaaaa atccggtgct
    15661 cgtgctgggt attggcggat ttattctcgg tattctgggg gttattggtg gaccagtttc
    15721 gctgtttaaa gggctggatg agatgcagca gatggtggca aatcaggctt tcagcaccag
    15781 cgattacttt ttgctggcgg taattaaact tgccgccctg gtcgttgctg ccgccagtgg
    15841 ctttcgcggt gggcgaatct tcccggcagt gtttgtcggc gtggcattag ggttgatgct
    15901 gcatgagcac gtacccgccg taccagcggc aataaccgtt tcttgcgcta ttctcggcat
    15961 cgtgctggtg gtaacacgcg atggctggtt aagtcttttt atggcggcgg tcgttgtacc
    16021 caataccaca ttgctaccgc tgctctgtat cgtcatgctt ccggcatggc tgttattagc
    16081 aggtaagccg atgatgatgg tcaatcgtcc gaagcaacag ccaccccacg ataacgttta
    16141 gcaaaaaatg ctctccttta tgatttaaga gttatggcga tgatacgggg ctttacctcc
    16201 ccgtaatatt gcgttaacag gccgctgacg gcttcgatcg tgaaggagaa taacaatgtt
    16261 caggtcactg tttctggcgg ccgccctgat ggcatttacc ccgcttgcag caaacgcagg
    16321 tgaaatcacc ctactgccat caatcaaatt acaaattggc gatcgcgatc attacggtaa
    16381 ttactgggac ggtggtcact ggcgcgaccg tgactactgg catcgcaatt atgagtggcg
    16441 taaaaaccgc tggtggcgtc atgataatgg ctatcatcgt ggctgggata agcgtaaagc
    16501 gtatgagcgt ggctatcgtg aaggctggcg cgatcgtgac gatcatcgcg gaaaaggccg
    16561 cggacatgga caccgccatt aaacacattc gccaatggaa cacaatgcct gatgcgacgc
    16621 tagcgcgtct tatcaggcct acaaaccggc tctttaacgc gtagggcgga taaggcgttt
    16681 acgccgcatc cgccaatagt gccagatgcg acgctcattc aactacaatc ccagcgccgt
    16741 ccccaccaac aaccagatat tcagcgccac gaccagcact acaatcaccc agcctgtctg
    16801 ttttacgcgt ttgctgttca ccagatcgcc catcaacttg ctgtcactgg tgaaaatcag
    16861 cagtggaacc agcgccaggg cgataccaaa acttaacagc acctgactca taaccagaat
    16921 ccgtgtcgga tctaatccca tcagaatgac aataaatgac ggcaacatgg tgactgtacg
    16981 acgcacccac agcgggatat ggaagcgaat gaatccctgc atcaccacct gccccgccag
    17041 tgtccccacc accgttgagg acagtccggc agcaaccaga cttaacccaa agaccgttgc
    17101 cgcagcatgg cttaacagcg gttgcagcgt cagataagcc tcatcaagat cggcaacacc
    17161 agtatgacca gaaaagtgga acgccgccgc agctgtagcc atcatcgcca gattgacaaa
    17221 accggcaatc gtcatggcga tagccacatc ccatttggtg gcggaataac gttgttgacg
    17281 cgaaccgcca tgtaaatgct gagtgagcga agagtgcaaa taaatcacat gcggcataat
    17341 cgtcgcccct aacacgcctg ctgccaggaa gaccgcctcc gaagtaggta aactcgggat
    17401 caccattcct ttacccagct gcgccaggtt aggctgggag aaaatcaact cgacaatgta
    17461 agccgcggca acaaacaaca gtaacccgcc aatcactttc tccagcggtt tttgcccgcg
    17521 acgttgcagc attaaaatca ggaaagtcgc gatccccgtc agcaccgcgc cctgcaacaa
    17581 cgaaacacca agaatgagtt taaaaccgat cgccgcacca ataaattccg ccaggtcggt
    17641 tgccatcgca ataatttctg cctgaaccca atagaaccac actacgggac gcggatagtg
    17701 atcacgaatc tgctccgcca gatttttacc ggtggcaatc cctagtttgg cagagaggat
    17761 ctgaatcagc atcgccatca ggttggccca aacgacaacc cacagtagct gatagccgaa
    17821 gctagcaccc gcctgaatat tggtcgcaaa gttaccggga tcgatataac caatcgccgc
    17881 aatgaacgca ggtcccatta atgcgagcct catcttgcgc gccgcccgtc cgctgctact
    17941 ctcaacgcga tagttcgtca tcttgtgcct ctaaaacata gcctttgcta tgtttcatgc
    18001 tatgccaaac gagaatgatt atcaaattca tttgaatggg ttgtggtgat ttctttgata
    18061 gaccaggatt atgactacga aaagattgat gacttgaatg ttatggttat gtttaatgtt
    18121 agcacattta cataactttc agcttccata cacaacatag cagaaatgta tgacagatca
    18181 ctatttttga agcttgtcac aggacgtcat tatagtgtgt gtcagatctc gttttcctta
    18241 accatgttac atagaatgtg cacggaaatt taacctgcct catatttgga gcaaatatgg
    18301 accgcgtcct tcattttgta ctggcacttg ccgttgttgc gattctcgca ctgctggtaa
    18361 gcagcgaccg caaaaaaatt cgtatccgtt atgttattca actgcttgtt atcgaagtgt
    18421 tactggcgtg gttcttcctg aactccgacg ttggtttagg cttcgtgaaa ggcttctccg
    18481 aaatgttcga aaaactgctc ggatttgcca acgaagggac taacttcgtc tttggtagca
    18541 tgaatgatca aggcctggca ttcttcttcc tgaaagtgct gtgcccaatc gtctttatct
    18601 ctgcactgat cggtattctc cagcacattc gcgtgttgcc ggttattatc cgcgcaattg
    18661 gtttcctgct ctccaaagtc aacggtatgg gcaaactgga atcctttaac gccgtcagct
    18721 ccctgattct gggtcagtct gaaaacttta ttgcctataa agatatcctc ggcaaaatct
    18781 cccgtaatcg tatgtacacc atggctgcca cggcaatgtc caccgtgtcg atgtccatcg
    18841 ttggtgcata catgaccatg ctggaaccga aatacgtcgt tgctgcgctg gtactgaaca
    18901 tgttcagcac ctttatcgtg ctgtcgctga tcaatcctta ccgtgttgat gccagtgaag
    18961 aaaacatcca gatgtccaac ctgcacgaag gtcagagctt cttcgaaatg ctgggtgaat
    19021 acattctggc aggtttcaaa gttgccatta tcgttgccgc gatgctgatc ggctttatcg
    19081 ccctgatcgc cgcgctgaac gcactgtttg ccaccgttac tggctggttt ggctacagca
    19141 tctccttcca gggcatcctg ggctacatct tctatccgat tgcatgggtg atgggtgttc
    19201 cttccagtga agcactgcaa gtgggcagta tcatggcgac caaactggtt tccaacgagt
    19261 tcgttgcgat gatggatctg cagaaaattg cttccacgct ctctccgcgt gctgaaggca
    19321 tcatctctgt gttcctggtt tccttcgcta acttctcttc aatcgggatt atcgcaggtg
    19381 cagttaaagg cctgaatgaa gagcaaggta acgtggtttc tcgcttcggt ctgaaactgg
    19441 tttacggctc taccctggtg agtgtgctgt ctgcgtcaat cgcagcactg gtgctgtaaa
    19501 accatacata aaaaagccgg ggatcattcc ccggcttttt tacgcctgtt aatcagctaa
    19561 tggctgcggg cgaccaatca aatacccttg cagatattgc accccgagct tatgcaatag
    19621 cgcctgctgc tgttgcgtct cgacaaactc cgcgaccaca ctcaatgact tcgctttcgc
    19681 cagatcggta attgatctca caatcatcgc atccagcgta ttcgtgacaa tatctttcac
    19741 aaagacgcca tcaattttga tgatatcagc ctgcaaacgc tttaaccgtt cgtagttggc
    19801 atatccggtg ccaaagtcat caatcgcaat ccggaaacca aacttatgca gctgctcgat
    19861 gttgtacatg ctgctttctg cgttagaaaa cgcctgctcc tcggtgatct caagaatgac
    19921 cgcctgcggg gagatgtgat aacgcttaaa cagacgaata atccgcccgg caatattctt
    19981 ttgcagcagc gtgagcggca ttaaattgac tgaaaagcgc ggtccttttt tgtcgcaagg
    20041 gtgtgttgcc agccacttca acaaggattc cagcacttgc aaatcaaaac gcgcgctaag
    20101 gttaaactga gcaataaggg gcagaaactt atccggggtc ataatgccgc cgtcatattt
    20161 cagtcgcgcg aggatctcat cataaccttc accctctttg ttgcgaattg gctgggcgta
    20221 gagcagcaaa tcaccctgat ctaacgccgt gcgaatggta ttcagcaata gcacctgttt
    20281 agtggtctgc ccggaaacca tctcctctct gctatccagc gccagcacat gatgatgtgc
    20341 gcaggattgc tccgccagcc agcttaactg ccccaacaag ggttgcaggg tttcctgatt
    20401 tccatcaaaa cgcccccagg cagcaccata gcccatatcc agcccggtat tgttccagtg
    20461 aatttgccga ctattcagga tgttaaccat atgctggagt cgcccttccg tttccggccc
    20521 gctcagcact aacagcagtt cactacccgg caattgatac aacttttcgt tttcctgcat
    20581 caacggcagc agcgtacggc aaattgagcg gatacagtga acgcgcatca ttaagccgta
    20641 atgacgactc ataaactcaa gattatcaat gcgcaggcag caaaaactct tgcccgcctc
    20701 ttgctccggc gcttgttcca acgcacgaaa gttgggtagg agcgttaacg gatctgtcag
    20761 cgcctgcaaa tgccagcggc gattaagcca ttcactgcga tgataaatgc gcaccatata
    20821 gagcaggcaa acgctaaagg aaatcagcac cgcaagaata aatgccagcg aatattcggt
    20881 ttccacccct tgcaaaaagt tctggttgta attcagaagg caaagcgttg aaacagccca
    20941 ggtgagattt aaaaacggat agcgaagctt accgaccccg agggtaaaga tgatgaagaa
    21001 aacgggtacc aagtaaccgg caataaagtc gttttcataa ggtgtgcaca acagaagtag
    21061 cagcacgctt aacgctgcca gccagcttaa ggtaaatgcg cgtttctctt tgcccaacga
    21121 cggagcgata tccctgcgcc acaatatctg cgcaaagtgg ggacttacaa tcatgcgagt
    21181 gagatagtag aaaagcatgt tgtaaatcag cacagcggtg aaaaggctta gcaaatcaac
    21241 gaccgtgaaa atggcatccg catcgccgaa aaaggtggat atctttagcg ggaaatcaaa
    21301 gaagctcccc acaagataca tgctgcattt gatgccgatc ggcgtcacca aaccaagcca
    21361 gaacagacgt tgccagacat atcggctggt caatccataa cgccaacgcg tccccagctg
    21421 ccagcgtaat attgcgcagg cacacaacac gacaaacgtc tggctaccga gcatgaccgc
    21481 agtctgtaaa aaagagagat gaaagttcca cagattggtg caaaacatcc cgcataaaac
    21541 gggaacgaca ccgcgccagc caaagataaa caagattgac agcattacgc acagtggcat
    21601 ccacgccaga aagatataac tggaatggat aatggcaagt ggcgaaataa aacgggatag
    21661 ctgaataagt accacggtga gcgtaaacgc tagtgtgaat atcttgatat tttttatcag
    21721 gttatgctcc acaaacatga gtacactact ctcactgatc ggggttttca ggcacacaaa
    21781 taataagttt attcgcatcg cgaagcaaac aggggccagc ctgttgccga tttttcatcc
    21841 aatgatgctt tcaccaggcg gcgctaaccc ggacgtagac tgtattacaa aagcggcaaa
    21901 aagcagagac aaaaaacccc cgctttgcag cgagggttgg aaatttggtg gagctaagcg
    21961 ggatcgaacc gctgacctct tgcatgccat gcaagcgctc tcccagctga gctatagccc
    22021 cacgatgcgt ttacgtacca aatttgctgg gtgcaaaatt tggtggagct aagcgggatc
    22081 gaaccgctga cctcttgcat gccatgcaag cgctctccca gctgagctat agccccgtca
    22141 cgtaaagctt gtcgagttga cgggcggcat catatgaatt ccgcccgaat gtgtcaacgg
    22201 caaattgcaa cgtgtagttt caatcgctga aaaatcaggc aaatgaacaa ttttggaaaa
    22261 ccgctcgcat tcagtagtta ttcatgtcac ggtttcctgt aaagtggtgt tataaaatga
    22321 actactaata gacccacata cattcaggga attgttatgt tcaaggagag gatgacacca
    22381 gatgaacttg ccagactgac cggctatagc cgccagacca ttaataaatg ggtacgcaag
    22441 gaaggctgga caacgtcacc aaaacctggc gtccagggtg gcaaagcccg actggttcac
    22501 gtcaatgaac aggttcgtga atatattcgc aatgctgaac gtccagaagg tcagggagaa
    22561 gcgcctgccc tttctggtga tgcgccgctt gaagtattgc tggtgacact ggcgaaagag
    22621 atgacgccag ttgaacaaaa acagtttaca tccttgcttc tgcgggaagg gattatcgga
    22681 ttgttacaac gcttagggat tcgcgatagc aaataatatg aaaagattac gcaacaaaat
    22741 gaccaccgaa gaactggctg aatgcctcgg tgtggctaaa caaactgtta accgttggat
    22801 cagagaaaaa ggctggaaaa cggaaaaatt tcccggcgtg aaaggcggtc gtgccagact
    22861 tattctggtc gatacgcaag tttgcgagtt tattcagaac acgccagcct tccataacac
    22921 accaatgttg atggaagctg aagagagaat tgctgaatac gctcctggag cacgcgcccc
    22981 ggcgtatcga caaatcatca acgcgataga taatatgaca gacattgagc aggaaaaagt
    23041 cgcgcaattt ttgtcacgtg aaggaattcg caatttcctc gcccgtctcg atattgacga
    23101 atcagcataa ataaaaaacg gcaggatatt atctcctgcc gtttatcttt ttacacgcta
    23161 attactgctg attttcgcgt tcagcaataa aatccagcgc tttgttgata cgctcgatac
    23221 tgcgggtctt accaattgcg tgaacggtaa catccagtgc tggagactgc cccgcaccgg
    23281 ttacggcgac acgcagcggc ataccaactt tacccatacc cacttccagc tcatccgccg
    23341 tcgcctgaat agcgtgatga acgttttcag cggtccagtc agtaatcgcg gccagtttgt
    23401 cacgaaccac ttccagcggc tgacgcgcta ccggacgcag atgttttttc gccgcatcgg
    23461 catcgaactc agcaaaatct tcgtagaaat aacggcagct ctgtgccatc tctttcagcg
    23521 tcttgcagcg ttcgcccagc agtttcacca gatcagccag ctgcgggccg ttacgggtat
    23581 cgatattttc ctgctcaatg tgccactgta agtgagtagc aacatactcc ggcggcagcg
    23641 cgttaatgta gtgatggttc agccacagca gcttgtcggt gttgaacgca ctggcagatt
    23701 tgctgacggc attcaaagtg aagtatttga tcatctcttc acgagtgaag atttcctgat
    23761 cgccgtggga ccagcccaga cgcaccagat agttcagcag tgcttctggc aaataaccgt
    23821 catcacgata ctgcattacg ctgactgccc cgtgacgttt ggacagtttt ttaccgtcat
    23881 cgccattgat catagaaacg tgcgcgtaaa ccggcaccgg cgctttcagg gccttaagaa
    23941 tgttgatctg gcgcggcgtg ttgttgatat ggtcttcgcc acggataacg tgggtgattt
    24001 ccatatccca gtcatcgaca accacacaga agttataggt tggggaacca tcggtacggc
    24061 ggataatcag atcatccagc tcctggttgc tgaactcgat cggaccacgg atctgatcgt
    24121 caaaaacaac agaaccttcc tgcggattag caaaacgcac gacacacggt tcatcatcag
    24181 catgatgctc atggctgtgg cggcagcgac cgtcataacg cggcttctca cctttcgcca
    24241 tttgctcttc gcgcagcgct tccaggcgct ctttagagca atagcattta taagcagtgc
    24301 cctcttccag catctgatcg atcaccgcat tgtagcgatc aaaacgtttg gtctggtagt
    24361 acggaccttc atcccactcc aggctcagcc agttcatgcc atccataatg gcttcgatag
    24421 cttccggcgt ggaacgctca agatcggtgt cttcaatacg cagcacgaac tcaccgccgt
    24481 ggttacgtgc aaaaagccag gagtaaagag cagtacgcgc gccgccaacg tgcagatagc
    24541 ctgttgggct tggcgcgaag cgagttttga ttttcatgaa atggccttac gtttagaaag
    24601 atgccgacaa ccggcaaatc ctggaaaaat tgagtgggcg atattctatc actcccgcct
    24661 gattcctcaa tgtagttcgg gtttctacct tacgcattat tgttttcgtt gacaaattgc
    24721 gcaacacggt ctgttttgcg atcatcttgc ttaaatttac gacgaacgaa caatttcttt
    24781 agaaaatgcg ttgactcatt ttgaactctc cctataatgc gaatccacac agcg
//
LOCUS       DAPHDW010000061        23955 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.061, whole genome shotgun sequence.
ACCESSION   DAPHDW010000061 DAPHDW010000000
VERSION     DAPHDW010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.061"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            36..3770
                     /gene="yfaL"
                     /locus_tag="RH057_002434"
     CDS             36..3770
                     /gene="yfaL"
                     /locus_tag="RH057_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
                     /protein_id="HDV3128789.1"
                     /translation="MRIIFLRKEYLSLLPSMIASLFSANGVAAAIDLCQGYDIKASCH
                     ASRQSLSGITQDWSVADGQWLVFSDMTNNASGGAVFLQQGAEFSLLPENETGMTLFAN
                     NTVTGEYNNGGAIFAKENSTLNLTDVIFSGNVAGGYGGAIYSSGTNDTGAVDLRVTNA
                     MFRNNIANDGKGGAIYTINNDVYLSDVIFDNNQAYTSTSYSDGDGGAIDVTDNNSDSK
                     HPSGYTIINNTAFTNNTAEGYGGAIYTNSATAPYLIDISVDDSYSQNGGVLVDENNSA
                     AGYGDGPSTAAGGFMYLGLSEVTFDIADGKTLVIGNTENDGAVDSIAGTGLITKTGSG
                     DLVLNADNNDFTGEMQIENGEVTLGRSNSLMNVGDTHCQDDPQDCYGLTIGSIDKYQN
                     QAELNVGSTQQTFVHSLTGFQNGTLNIDAGGNVTVNQGSFAGTIEGAGQLTIAQNGSY
                     VLSGAQSMALTGDIVVDDGAVLSLEGDAADLAALQDDPQSIVLNGGVLDLSDFSTWQS
                     GTSYNDGLEVSGSSGMVIGSQDVVDLAGGNDMHIGGDGKDGVYVVVDAGDGQVSLANN
                     NSYLGTTQIASGTLMVSDNSQLGDTHYNRQVIFTDKQQESVMEITANVDTRSTTTEHG
                     RDIEMRADGEVAVDAGVDTQWGALMADSSGQHQDEGSTFTKTGAGTLELTASGTTQSA
                     VRVEEGTLKGDVADIFPYASSLWVGDGATFVTGADQDIQSIDATSSGTIDISDGTVLR
                     LTGQDTSVALNASLFNGDGTLVNATDGVTLTGELNTNLETDSLTYLADVTVNGDLTNT
                     SGAVSLQNGVAGDTLTVNGDYTGGGTLLFDSELNGDDSVSDQLVMNGNTAGNTTVVVN
                     SITGIGEPTSTGIKVVDFAADPTQFQNNAQFCLAGSGYVNMGAYDYTLVEDNNDWYLR
                     SQEVTPPSPPDPDPDPTPDPDPTPDPIPAYQPVLNAKVGGYLNNLRAANQAFMMERRD
                     HAGGNGQTLNLRVIGGRYHYTAAGQLAQHEDTSTVQLSGDLFSGRWGDDGEWMLGAVG
                     GYSDNQGESRSNMTGTRADNQNHGYAVGLTSSWYQHGNQKQGAWLDSWLQYAWFNNDV
                     SEQDDGTDHYHSSGIIASLEAGYQWLPGRGVVIEPQAQVIYQGVQQDDFTAANHARVS
                     QSQGDDIQTRLGLHSEWRTAVGVTPTLDLNYYHDPHATEIEEDGSTISDDAVKQRGEI
                     KVGVTGNISQRVSLRGSVAWQKGSDDFAQTAGFLSMTVKW"
     gene            complement(3898..4620)
                     /locus_tag="RH057_002435"
     CDS             complement(3898..4620)
                     /locus_tag="RH057_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HDV3128790.1"
                     /translation="MNAEKSPVNHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     LTNTFKLGPGVDVNYMLHTQNK"
     gene            4767..7394
                     /gene="gyrA"
                     /locus_tag="RH057_002436"
     CDS             4767..7394
                     /gene="gyrA"
                     /locus_tag="RH057_002436"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HDV3128791.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDSAVYDTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEHIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7543..9231
                     /locus_tag="RH057_002437"
     CDS             7543..9231
                     /locus_tag="RH057_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
                     /protein_id="HDV3128792.1"
                     /translation="MSGEKKAKGWRFYGLVGFGAIALLSAGVWALQYAGSGPEKTLSP
                     LVVHNNLQIDLNEPDLFLDSDSLSQLPKDLLTIPFLHDVLSEDFVFYYQNHADRLGIE
                     GSIRRIVYEHDLTLKDKLFSSLLDQPAQAALWHDKQGHLSHYMVLIQRSGLSKLLEPL
                     LFAATSDSQLSKTEISSIKINSETVPVYQLRYNGNNALMFATYQDKMLVFSSTDMLFK
                     DDQQDTEATAIAGDLLSGKKRWQASFGLEERAAEKTPVRQRIVVSARLLGFGYQRLMP
                     SFAGVRFEMGNDGWHSFVALNDESASVDTSFDFTPVWNSMPAGASFCVVVPYSHGIAE
                     EMLSHISQENDKLNGALDGAAGLCWYADSKLQTPLFVGQFDGTAEQAQLPGKLFTQNI
                     GAHESKAPEGVLPISQTQQGEAQIWRREVSSRYGQYPKAQAAQPDQLMSDYFFRVSLA
                     MQNKTLLFSLDETLVNNALQTLNKNRPAMVDVIPTDGIVPLYINPQGVAKLLRNETLT
                     SLPKNLEPVFYNAAQTLLMPKLDALSQQPRYVMKLAQMEPGAAWQWLPITWQPL"
     gene            9228..9851
                     /locus_tag="RH057_002438"
     CDS             9228..9851
                     /locus_tag="RH057_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HDV3128793.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSGLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGFSSQYLPPEMTLTPGQRQLAQNW
                     NQGNGKTGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            9875..14389
                     /locus_tag="RH057_002439"
     CDS             9875..14389
                     /locus_tag="RH057_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000536590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HDV3128794.1"
                     /translation="MGTGLANADDSLPSSNYAPPAGGTFFLLADSSFSSSEEAKVRLE
                     APGRDYRRYQMEEYGGVDVRLYRIPDPMAFLRQQKNLHRIVVQPQYLGDGLNNTLTWL
                     WDNWYGKSRRVMQRTFSSQSRQNVTQALPELHLGNAIIKPSRYVQNNQFSPLKKYPLV
                     EQFRYPLWQAKPVEPQQGVKLEGASSNFISPQPGNIYIPLGQQEPGLYLVEAMVGGYR
                     ATTVVFVSDTVALSKVSGNELLVWTAGKKQGEAKPGSEILWTDGLGVMTRGVTDDSGT
                     LQLQHISPERSYILGKDAEGGVFVSENFFYESEIYNTRLYIFTDRPLYRAGDRVDVKV
                     MGREFHDPLHSSPIVSAPAKLSVLDANGSLLQTVDVTLDARNGGQGSFRLPENAVAGG
                     YELCLAYRNQVYSSSFRVANYIKPHFEIGLALDKKEFKTGEAVSGKLQLLYPDGEPVK
                     NARVQLSLRAQQLSMVGNDLRYAGRFPVSLEGSETVSDASGHVALNLPAADKPSRYLL
                     TVSASDGAAYRVTTTKEILIERGLAHYSLSTAAQYSNSGESVVFRYAALESSKQVPVT
                     YEWLRLEDRTSHSGELPSGGKSFTVNFAKPGNYNLTLRDKDGLILAGLSHAVSGKGST
                     AHTGTVDIVADKTLYQPGETAKMLITFPEPIDEALLTLERDRVEQQSLLSHPANWLTL
                     QRLNDTQYEARVPVSNSFAPNITFSVLYTRNGQYSFQNAGIKVAVPQLDIRVKTDKTH
                     YQPGELVNVELTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRNNVRT
                     SSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDKQGKA
                     YFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDKPAAG
                     LFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLAQNIQQSGLLSAELQQNGQV
                     QDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFRNNLD
                     ALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAGPGAR
                     FTWWGEDGNGDAFLTAWAWYADWQASQAIGVTQQPEYWQHMLDSYAEQADNMPLLHRA
                     LVLAWAQEMNLPCKTLLKGLDEAIARRGTKTEDFSEEDTRDINDSLILDTPESPLADA
                     VANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQTAAIL
                     SGLTAEQSTIERALAMNWLAKYMATMPPVVLPAPAGAWAKHKLTGGGEDWRWVGQGVP
                     DILSFGDELSPQNVQVRWREPAKTAQQSNIPVTVERQLYRLIPGEEEMSFTLQPVTSN
                     EIDSDALYLDEITLTSEQDAVLRYGQVEVPLPPGADVERTTWGISVNKPNAAKQQGQL
                     LEKARNEMGELAYMVPVKELTGAVTFRHLLRFSQKGQFVLPPARYVRSYAPAQQSVAA
                     GSEWTGMQVK"
     gene            14390..16039
                     /locus_tag="RH057_002440"
     CDS             14390..16039
                     /locus_tag="RH057_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HDV3128795.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLIRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPGVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QDLIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            16044..16820
                     /locus_tag="RH057_002441"
     CDS             16044..16820
                     /locus_tag="RH057_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HDV3128796.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRVESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            17095..19944
                     /gene="rcsC"
                     /locus_tag="RH057_002442"
     CDS             17095..19944
                     /gene="rcsC"
                     /locus_tag="RH057_002442"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HDV3128797.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPGVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRES"
     gene            complement(20144..20794)
                     /gene="rcsB"
                     /locus_tag="RH057_002443"
     CDS             complement(20144..20794)
                     /gene="rcsB"
                     /locus_tag="RH057_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HDV3128798.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(20811..23483)
                     /gene="rcsD"
                     /locus_tag="RH057_002444"
     CDS             complement(20811..23483)
                     /gene="rcsD"
                     /locus_tag="RH057_002444"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HDV3128799.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTWQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDTTATGDNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESLPNTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVDE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRQLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
BASE COUNT         5825 a         5826 c         6550 g         5754 t
ORIGIN      
        1 tatgccgtct tatataaaat gatggtcaga ttaatatgcg gattatcttt ctacgcaagg
       61 agtatttatc tctactcccg tcaatgattg catctctttt ctctgctaac ggtgtcgcgg
      121 cggccattga tttatgccag ggatatgata tcaaagcgag ttgtcacgcc agcaggcaaa
      181 gcctttcagg cattacgcag gactggagtg tcgccgatgg gcaatggctg gttttttcgg
      241 atatgaccaa taatgccagc ggaggagccg tatttttgca acaaggagcg gaattttcac
      301 tattaccaga aaatgaaact ggaatgactc tgtttgccaa taacaccgtt acaggagaat
      361 ataataacgg cggggccata tttgctaaag aaaactcaac gctgaatctt actgatgtta
      421 ttttttccgg taacgtcgca ggcggctatg gtggcgcaat ctattcttct ggtactaacg
      481 atactggtgc cgtcgattta cgtgtcacta acgccatgtt tcgcaataac atcgctaatg
      541 atggcaaagg tggcgcaatt tataccatta ataatgacgt ttatttaagt gatgttattt
      601 ttgataacaa ccaggcatat acatcaacaa gttacagtga tggcgatggc ggggcaatcg
      661 atgttaccga taataatagc gacagcaagc atccttcagg ttatacgata ataaataaca
      721 ccgcctttac aaataacact gccgaaggtt atggcggggc gatatatacc aatagcgcga
      781 cggctcccta tcttattgat atttctgttg atgacagcta cagccagaac ggcggcgtgt
      841 tagtggatga gaacaacagc gcagcaggct atggagatgg tccttccact gcggcgggtg
      901 gctttatgta tctcggctta agtgaagtta cctttgatat tgccgacgga aaaacgctgg
      961 ttattggcaa tacagagaat gacggagctg ttgactctat tgctggtacc gggttgatca
     1021 ccaaaacagg ttccggcgat ctggtactta atgcagataa caatgacttt actggtgaga
     1081 tgcagattga aaacggtgaa gttaccctgg gtcgcagcaa ctccctgatg aatgtcggcg
     1141 atacgcattg ccaggacgat ccgcaagact gctacggtct gacgataggg agtattgata
     1201 agtaccagaa tcaggcagag ctaaacgttg gctccaccca acaaaccttt gtgcactcat
     1261 tgacgggctt tcagaatggc actttaaata tcgatgctgg tggcaatgtt actgttaatc
     1321 aaggtagttt tgctggcacc atcgaaggtg ctggtcagct caccattgcg caaaacggca
     1381 gctacgtgct gtcgggggcg cagtcgatgg cgctaaccgg cgatatagtc gttgatgatg
     1441 gtgcggtgct ttcgctggaa ggcgacgcgg cagatctcgc cgctctccag gacgatccgc
     1501 agtcgatcgt gttaaacggc ggtgtgctcg atctctctga tttctccacc tggcagagtg
     1561 gcacatcata caacgatggc cttgaagtca gcggcagcag cggaatggtt atcggcagtc
     1621 aggatgtggt agatcttgca ggtggaaacg atatgcatat cggcggtgac gggaaagatg
     1681 gcgtctacgt ggtggtcgat gcgggcgacg ggcaggtaag tctggcaaac aataatagtt
     1741 atttgggcac aacacaaatc gcctccggta cgctgatggt gagcgacaac tcgcagcttg
     1801 gagataccca ctataaccgc caggttatct ttaccgataa gcaacaagaa agcgtgatgg
     1861 agattactgc caatgtcgat actcgctcta cgacgactga gcatgggcgt gatattgaaa
     1921 tgcgcgccga cggtgaagtg gcagttgatg cgggggtaga cacgcagtgg ggcgcactga
     1981 tggctgacag cagcgggcag catcaggatg agggtagcac atttactaaa acgggggcgg
     2041 gtacgctgga gctgaccgcc agcggtacaa cgcagtcggc ggtacgtgtc gaagaaggca
     2101 ccctgaaagg cgatgttgcg gatatctttc cttatgcttc gtcactgtgg gttggtgatg
     2161 gggcgacgtt cgttactggc gcggatcagg atattcagtc tatcgatgct acttccagcg
     2221 gcactatcga tatcagcgat ggtacggttt tgcgtctgac cgggcaggat acttccgtcg
     2281 cccttaatgc ctcattgttt aacggcgatg ggacgctggt gaatgctacc gatggcgtga
     2341 cgttgacagg tgagcttaat accaaccttg aaactgacag cctgacctat cttgccgacg
     2401 tgacggttaa tggtgatctg accaatacgt ccggtgcggt tagcctgcaa aatggcgtcg
     2461 ctggcgatac gctgacggta aacggtgatt ataccggcgg cggtacgcta ctgttcgaca
     2521 gcgaattaaa cggcgatgac tcggtaagcg atcaattggt gatgaacggt aatactgctg
     2581 gcaacacaac tgtggtggtt aactccatta cagggattgg tgagccgaca tcgacaggca
     2641 ttaaagtggt tgatttcgca gctgatccca cgcagtttca aaacaatgca cagttctgtc
     2701 tggctggcag tggctacgtc aacatgggag cgtatgacta cacgctagtg gaagataaca
     2761 acgactggta tctgcgatcg caagaagtaa cgccgccatc gccacctgat ccagaccctg
     2821 acccaacacc cgatcctgac ccaacacccg atccaatccc cgcttaccag ccggtgctga
     2881 atgccaaagt tggcggttat ctcaataatc tgcgggcggc aaatcaggcg tttatgatgg
     2941 agcgacgcga tcatgcaggt ggcaatggtc agacgctgaa tttacgtgtt atcggcggac
     3001 gttatcatta cacggcggca gggcaactgg ctcagcatga agacacttct acggtgcagc
     3061 ttagcggtga cctgtttagc gggcgctggg gcgatgatgg cgagtggatg cttggggcgg
     3121 ttggtggcta cagtgataat cagggcgaga gccgctcgaa tatgaccgga actcgcgccg
     3181 ataaccagaa ccacggttat gccgttgggc tgacctcaag ctggtatcag cacggtaatc
     3241 agaagcaggg ggcctggctg gatagctggc tgcaatacgc gtggtttaac aatgatgttt
     3301 ccgaacaaga cgatggcaca gatcattacc actcgtcggg gattatcgcc tcgctggaag
     3361 ccgggtatca gtggttaccg gggcgtggtg tggtgattga accgcaggcg caggtgattt
     3421 atcagggcgt gcagcaggat gattttaccg ccgctaacca tgcgcgcgtg tcacaatcgc
     3481 agggggatga tattcagacg cggctgggtt tacacagcga atggcgtacc gcggttggtg
     3541 tcacaccaac attagatctg aattattatc acgatcccca tgcgacggaa attgaagaag
     3601 atggcagcac tatcagtgac gatgcggtga agcaacgggg tgaaataaaa gtgggagtca
     3661 cgggcaatat cagtcagcga gtgtccctgc gcggcagcgt ggcgtggcag aaagggagtg
     3721 atgattttgc ccagacggca ggttttttgt cgatgacggt gaaatggtaa tcgctgtctg
     3781 accatcacta ttgccggatg cggcgtaaac gccttatccg gcctacgggg agcatttgta
     3841 ggcctgataa gacgcggcaa gcgtcgcatc aggcattggc tcggccaaac aacaacctca
     3901 cttattctgc gtgtgcagca tatagttcac atccacgccg gggccgagtt taaaagtatt
     3961 agtgagcggg ttgtaatgta gcccggttat atgtcgctct ttaaggcttg tctgatctac
     4021 ccagccaagc agttctgccg gtttaataaa cttcttcaca tcatgcgtgc ctttgggcac
     4081 catgcgcaaa atatattccg cgccaaccac cgccatcagc catgacttgc cgttgcggtt
     4141 aagcgtcgag aaaaagacat cgccgcctgg tttcaccagt tgcgcacagg ctctgaccac
     4201 tgactgcgga tcggggacgt gctccagcat ctccatgcag gtcaccacat catactgccc
     4261 ggcatgtttt gccgcgtgct cttccacggt ttcctgcacg taatccacct gaatgccgct
     4321 ttccagtgcg tgcagttttg ccacctgcaa tggctcaaag cccatatcca gaccggtcac
     4381 cgtcgcgcct tcgcgcgcca tactctcggc cagaatgccg ccgccacaac cgacatcgag
     4441 cacctttttg ccaaataaac cgccagcacg ctcggcaata tagcccagac gcagcgggtt
     4501 aatgcggtgc agcggtttga actcaccttc cagatcccac cagcgggagg cgacggcttc
     4561 aaatttagcg atctcttcgt ggtctacgtt atggtttacc ggcgattttt cggcattcat
     4621 tagcacttct actccgtaat tggcaagaca aacgagtata tcaggcattg gatgtgaata
     4681 aagcgtatag gtttacctca aactgcgcgg ctgtgttata atttgcgacc tttgaatccg
     4741 ggatacagta gagggatagc ggttagatga gcgaccttgc gagagaaatt acaccggtca
     4801 acattgagga agagctgaag agctcctatc tggattatgc gatgtcggtc attgttggcc
     4861 gtgcgctgcc agatgtccga gatggcctga agccggtaca ccgtcgcgta ctttacgcca
     4921 tgaacgtact aggcaatgac tggaacaaag cctataaaaa atctgcccgt gtcgttggtg
     4981 acgtaatcgg taaataccat ccccatggtg actcggcggt ttatgacacg atcgtccgta
     5041 tggcgcagcc attctcgctg cgttacatgc tggtagacgg tcagggtaac ttcggttcca
     5101 tcgacggcga ctctgcggcg gcaatgcgtt atacggaaat ccgtctggcg aaaattgccc
     5161 atgaactgat ggccgatctc gaaaaagaga cggtcgattt cgttgataac tatgacggca
     5221 cggaaaaaat tcctgacgtc atgccgacca aaattcctaa cctgctggtg aacggttctt
     5281 ccggtatcgc cgtaggtatg gcaaccaaca tcccgccgca caacctgacg gaagtcatca
     5341 atggttgtct ggcgtatatc gatgatgaag acatcagcat tgaagggctg atggaacaca
     5401 tcccgggtcc ggacttcccg acggcggcaa tcattaacgg tcgtcgcggt attgaagaag
     5461 cttaccgtac cggtcgcggc aaggtatata tccgcgcccg tgctgaagtg gaagttgacg
     5521 ccaaaaccgg acgtgaaacc attatcgtcc acgaaattcc gtatcaggta aacaaagcgc
     5581 gcctgatcga gaagattgcg gaactggtaa aagaaaaacg cgtggaaggc atcagcgcgc
     5641 tgcgtgacga gtctgacaaa gacggtatgc gcatcgtgat tgaagtgaaa cgcgacgcgg
     5701 tcggtgaagt tgtgcttaac aacctctact cccagaccca gttgcaggtt tctttcggta
     5761 tcaacatggt ggcattgcac catggtcagc cgaagatcat gaacctgaaa gacatcatcg
     5821 cggcgtttgt tcgtcaccgc cgtgaagtgg tgacccgtcg tactattttc gaactgcgta
     5881 aagctcgcga tcgtgctcat atccttgaag cattagccgt ggcgctggcg aacatcgacc
     5941 cgatcatcga actgatccgt catgcgccga cgcctgcaga agcgaaaact gcgctggtgg
     6001 ctaatccgtg gcagctgggc aacgttgccg cgatgctcga acgtgctggc gacgatgctg
     6061 cgcgtccgga atggctggag ccagagttcg gcgtgcgtga tggtctgtac tacctgaccg
     6121 aacagcaagc tcaggcgatt ctggatctgc gtttgcagaa actgaccggc cttgagcacg
     6181 aaaaactgct cgacgaatac aaagagctgc tggatcagat cgcggaactg ttgcgtattc
     6241 ttggtagcgc cgatcgtctg atggaagtga tccgtgaaga gctggagctg gttcgtgaac
     6301 agttcggtga caaacgtcgt actgaaatca ccgccaacag cgcagacatc aacctggaag
     6361 atctgatcac tcaggaagat gtggtagtga cgctctctca ccagggctac gttaagtatc
     6421 agccgctttc tgaatacgaa gcgcagcgtc gtggcgggaa aggtaaatct gccgcacgta
     6481 ttaaagaaga agactttatc gaccgactgc tggtggcgaa cactcacgac catattctgt
     6541 gcttctccag ccgtggtcgc gtctattcga tgaaagttta tcagttgccg gaagccactc
     6601 gtggcgcgcg cggtcgtccg atcgtcaacc tgctgccgct ggagcaggac gaacgtatca
     6661 ctgcgatcct gccagtgacc gagtttgaag aaggcgtgaa agtcttcatg gcgaccgcta
     6721 acggtaccgt gaagaaaact gtcctcaccg agttcaaccg tctgcgtacc gccggtaaag
     6781 tggcgatcaa actggttgac ggcgatgagc tgatcggcgt tgacctgacc agcggcgaag
     6841 acgaagtaat gctgttctcc gctgaaggta aagtggtgcg ctttaaagag tcttctgtcc
     6901 gtgcgatggg ctgcaacacc accggtgttc gcggtattcg cttaggtgaa ggcgataaag
     6961 tcgtctctct gatcgtgcct cgtggcgatg gcgcaatcct caccgcaacg caaaacggtt
     7021 acggtaaacg taccgcagtg gcggaatacc caaccaagtc gcgtgcgacg aaaggggtta
     7081 tctccattaa ggttactgaa cgtaatggtt tagtggttgg cgcggtacag gtagatgact
     7141 gcgaccagat catgatgatc accgatgccg gtacgctggt acgtactcgc gtttcggaaa
     7201 tcagcatcgt aggccgtaac acccagggcg tgatcctcat ccgtactgcg gaagatgaaa
     7261 acgtagtggg tctgcaacgt gttgctgaac cggttgacga ggaagatctg gataccatcg
     7321 atggcagtgc cgcggaaggg gacgatgaaa tcgctccgga agtggacgtt gacgacgagc
     7381 cagaagaaga ataattttac ttcttcatgc caaaagggag ctatctccct tgtttgaatt
     7441 gaaaagtcca ggctgcaaag tctgggcttt tgtcgtatta gggcacggta aagtttggct
     7501 gtgcccgtaa aaaatggctg gctatacaca aggaatgtgg caatgagtgg tgaaaaaaag
     7561 gcgaaaggct ggcggttcta tggtcttgta ggttttggcg caatagcact gctttccgct
     7621 ggcgtctggg cgttgcaata tgctggcagt gggccagaaa aaacgttgtc gccgctggtg
     7681 gtgcacaaca atctgcaaat cgatctcaat gagccggacc tctttctcga cagcgactct
     7741 ctgagccagc ttcccaaaga tctcctcacc attccgtttc tccacgatgt tctgagcgaa
     7801 gatttcgttt tctattatca gaatcatgcc gatcgtctgg gcattgaagg cagcattcgt
     7861 cgtattgtct atgaacacga tctcacgctg aaagataagc tcttttcgtc actcttagat
     7921 cagcccgcgc aggcagcgct gtggcacgat aaacaaggcc atctttcaca ttatatggtg
     7981 ctgatccagc gcagtggttt aagcaaactg ctggagccat tgttgtttgc cgctaccagc
     8041 gacagccagt taagcaaaac ggaaatcagt agcatcaaga taaatagtga aactgttcct
     8101 gtttatcagt tgcgctataa cggcaataac gctctgatgt tcgcgactta tcaggacaag
     8161 atgctggtgt tttccagcac ggatatgttg tttaaagatg atcagcagga taccgaagcc
     8221 acggcgatcg caggtgattt gttgagcggc aaaaaacgct ggcaagcaag ctttggcctg
     8281 gaagagcgtg ctgctgaaaa aacgccagta cgccagcgca tcgtggtcag cgccaggttg
     8341 ctgggttttg gctaccagcg gttaatgcct tcttttgctg gcgtacgctt cgaaatgggt
     8401 aacgacggct ggcacagttt tgtggcgtta aatgatgaat ccgccagcgt agataccagt
     8461 ttcgatttta cgccggtctg gaacagtatg cctgccgggg ccagcttctg tgtggtggtg
     8521 ccgtattcac acggtattgc cgaagagatg ctttcgcaca tcagtcagga aaacgacaag
     8581 ttgaatgggg cgttagacgg tgccgcgggg ctgtgctggt atgcagactc aaaattgcaa
     8641 accccgctgt ttgtcggtca gtttgatggc actgccgaac aggcgcaatt gccagggaaa
     8701 ctgtttacgc aaaatattgg tgcgcacgaa agcaaagcgc cagaaggtgt tttgccgata
     8761 agccagactc agcagggcga agcgcaaatc tggcgtcgcg aagtgagttc ccgatacggt
     8821 cagtatccga aagcgcaggc ggcgcaaccc gatcaattaa tgtcggatta ttttttccgc
     8881 gtgtcgctgg cgatgcaaaa caaaacgctg cttttctccc tcgatgaaac gctggttaat
     8941 aacgccctgc aaacactgaa taaaaaccgc ccggcaatgg tggatgtaat acccactgat
     9001 ggcatcgttc cgctctatat caatccacaa ggcgtggcga aactgctgcg taacgaaacg
     9061 ttgaccagtc tgccgaagaa tctcgaaccg gttttttata acgccgcaca aactttatta
     9121 atgccgaagc tggacgcttt atctcaacaa ccgcgttatg tcatgaagct ggcccagatg
     9181 gaacccggtg ccgcctggca gtggctaccc ataacctggc agccgctatg aggcacgggc
     9241 tgctggcgct gatttgctgg ctgtgttgtg ttgttgccca tagcgaaatg ctgaatgtcg
     9301 aacaatccgg gctgtttcgc gcctggtttg tacgcattgc acaagaacag ctccgccagg
     9361 ggccaagtcc acgctggtat cagcaggatt gtgcgggcct ggtgcgattt gcggcgaacg
     9421 agacgctgaa agttcacgat agtaaatggc taaaaagtaa cggtttctct agccagtatt
     9481 tgccgccaga gatgacgcta acacctggac agcgtcaact ggcgcaaaac tggaatcagg
     9541 ggaacgggaa aaccggcccc tacgtgaccg cgattaattt gattcagtac aacagccagt
     9601 ttattggcca ggacataaac caggcgctgc ctggcgatat gatttttttc gatcagggcg
     9661 atgcccagca cttaatggtc tggatggggc gttacgtcat ctaccacacc ggaagcgcca
     9721 cgaaaactga caacggaatg cgcgcagtca gtctgcaaca acttatgaca tggaaggaca
     9781 cccgatggat acccaacgat tccaatccca atttcattgg catttatcgt ttaaattttc
     9841 tggcgcgata gcagcgtgct tgtccctgtc tcttgtggga acagggcttg ctaatgctga
     9901 tgattcgctt ccttccagta actatgcgcc gcccgccggg ggaacattct ttttgcttgc
     9961 tgacagcagt tttagcagca gtgaagaggc gaaagtgcga ctggaagcgc cggggcgtga
    10021 ttatcggcgc tatcagatgg aagagtacgg cggcgtggac gttcgcctgt atcgtattcc
    10081 tgacccgatg gcatttttgc gccagcagaa aaacctgcat cgcattgtgg tgcaaccgca
    10141 atatctgggc gacgggctga acaatacgct gacctggctg tgggataact ggtacggcaa
    10201 atctcgccgc gtaatgcagc gtactttctc ttctcagtca cggcagaatg tgactcaggc
    10261 attacccgaa ttacatctcg gcaatgccat tattaaacct tcccgttatg tacagaacaa
    10321 ccagttttcc ccgctgaaaa aatatccctt ggtggaacag ttccgttatc cactatggca
    10381 ggctaaaccg gtcgagccgc agcaaggggt aaaactggaa ggcgcatcca gcaatttcat
    10441 ctcgccgcag ccgggtaaca tttatattcc tctcggccaa caagagccgg gactgtacct
    10501 cgtcgaggcg atggttggtg ggtatcgggc gacgacggtg gtgtttgttt ccgataccgt
    10561 ggcgcttagc aaagtgtcag gcaacgagct tctggtgtgg accgcgggta aaaaacaggg
    10621 tgaagcgaag cccggctcag agatcttgtg gactgacggt cttggcgtga tgacccgcgg
    10681 tgtgaccgat gacagcggta ccttgcagtt acaacatata tcgccagaac gttcatacat
    10741 tctgggtaag gatgctgaag gcggtgtttt tgtctccgag aacttcttct acgaaagcga
    10801 aatctacaac acccgcttgt atatttttac cgatcgcccg ctatatcgcg caggcgatcg
    10861 tgtcgatgtt aaagtgatgg gccgcgagtt ccacgatccg ttgcattcat cccccatcgt
    10921 cagcgccccg gcgaagcttt cggtgctgga cgctaacggc agtctgttgc aaaccgtcga
    10981 tgtcacgctg gatgcgcgca atggcgggca gggaagtttc cgcctgccag aaaatgccgt
    11041 tgccggaggt tatgagttat gtcttgctta ccgcaatcag gtctatagca gcagttttcg
    11101 cgtggcaaac tacatcaagc cacatttcga gattggttta gctctcgaca aaaaagagtt
    11161 caaaactggc gaagcggtca gcggcaaact gcaactgctc tacccggatg gcgagccggt
    11221 aaaaaatgcc cgcgtgcagt taagtttgcg cgctcagcaa ttatcaatgg tcggtaacga
    11281 tttgcgttat gccggacgtt tccccgtgtc gctggaaggc agcgaaacgg tgtccgacgc
    11341 cagcggtcat gtggcgttaa atctccccgc cgccgataaa ccgagccgtt atttgttaac
    11401 cgtctccgcc agtgacggcg cggcgtatcg tgtcaccacc accaaagaga tcctcattga
    11461 acgcggtctg gcgcattact cattaagtac tgccgcacaa tacagtaata gcggcgagtc
    11521 ggttgtgttc cgttatgccg cgctggaatc ttcaaaacag gttcctgtta cgtatgaatg
    11581 gttgcgtctc gaagaccgca cgagccatag cggagagcta ccgtcaggcg gcaaatcctt
    11641 taccgtcaat ttcgctaaac ctggcaacta caatctgaca ttacgcgata aagacggctt
    11701 aattctcgct gggttaagtc atgccgtcag cggtaagggc agcacggcgc atactggtac
    11761 ggtagatatc gtggcggata aaacgctgta ccagccaggc gaaactgcga agatgctgat
    11821 tacctttccg gagccaattg atgaagcatt attgacgctg gaacgcgatc gcgtggaaca
    11881 gcagtcgctg ctttcgcatc cggcaaactg gctgacgtta caacgtttaa acgataccca
    11941 gtatgaagcc cgggttccag tgagcaattc ctttgcgcct aacatcactt tttcggtgct
    12001 gtatacccgt aatggtcagt acagttttca gaacgccggg atcaaagttg ccgttcctca
    12061 gcttgatatc cgggtgaaaa cggacaaaac ccattaccag cctggtgaac tggtcaatgt
    12121 cgaattaacc tcgtcgctga aaggtaaacc tgtttctgcg cagctaacgg taggcgtggt
    12181 cgatgaaatg atctacgcgc tgcaaccaga aatcgcgccg aatatcggca aatttttcta
    12241 tccgctgggg cgtaacaatg tgcgtaccag ctccagtctg tcgtttatca gctacgacca
    12301 ggcgctctcc agcgagccgg ttgcgcctgg cgcaactaac cgcagcgagc ggcgagtaaa
    12361 aatgcttgaa cgtccacggc gtgaagaggt ggataccgcg gcatggatgc cgtcactcac
    12421 aaccgataaa caaggcaaag cgtatttcac gttcctgatg cctgattcgt taacccgctg
    12481 gcgtatcacc gcgcgtggga tgaacggcga cgggctggtc gggcaggggc gtgcttatct
    12541 gcgttcggaa aaaaatctct acatgaagtg gagtatgcca acggtgtatc gcgtgggcga
    12601 caaaccggcg gcaggactgt ttatcttcag tcagcaggat aacgaaccgg tggcgctggt
    12661 gactaaattt gcaggcgctg agatgcgcca gacgctgacg ctgcacaaag gggcgaatta
    12721 tatttcgctg gcgcagaaca ttcagcaatc tggcttgtta agcgcagaac tgcaacaaaa
    12781 tgggcaagtg caggacagca ttagcacaaa actgtctttt gtggataaca gctggcccgt
    12841 tgaacagcag aaaaatgtca tgctcggcgg tggcgagaac gcgctgatgt tgcccgagca
    12901 ggcgagcaat atccggctac aaagtagtga aacgccgcag gagattttcc gcaacaatct
    12961 tgatgcgtta gtcgatgaac cgtggggtgg cgtaatcaac accggtagcc gtctgatccc
    13021 gctcagtctc gcctggcgtt cgcttgccga tcatcaaagt gccgccgcta acgacattcg
    13081 tcagatgatt caggataacc gtctgcggct gatgcaactg gcggggcccg gagcgcgctt
    13141 tacctggtgg ggtgaagatg gcaatggtga cgccttcctt acggcatggg catggtacgc
    13201 cgactggcag gccagccagg cgatcggcgt aacgcaacaa ccggaatact ggcagcatat
    13261 gctcgacagc tacgcggagc aggcagataa catgccgtta ttgcatcggg cgctggtgct
    13321 ggcatgggcg caggagatga atttgccgtg caaaacgttg ttgaaagggt tggatgaagc
    13381 tatcgcccgg cgcggaacta aaactgaaga tttctctgag gaagacaccc gcgatatcaa
    13441 tgatagcctg atcctcgata caccggagtc tccactggca gatgcggtgg caaacgtctt
    13501 aaccatgacg ttgctgaaaa aagcgcagtt gaagtccacg gtgatgccac aggttcagca
    13561 atatgcgtgg gataaagcgg caaacagcaa tcagccgctg gcgcacacgg ttgtgctgct
    13621 taatagcggt ggcgacgcta cccagacggc cgctatttta agtggtttga ccgctgagca
    13681 atccactatt gagcgcgcgc tggccatgaa ctggctggcg aaatatatgg cgacaatgcc
    13741 tccagttgtt ttgcctgcgc ctgcgggcgc atgggctaaa cataagttaa ctggaggggg
    13801 cgaagactgg cgttgggttg gtcagggtgt gccggacatt ctctcttttg gtgacgaatt
    13861 atcgccgcaa aatgtgcagg tccgctggcg tgaaccggca aaaacggctc aacaaagtaa
    13921 cattccggtg accgttgaac gccagttgta tcggcttatc cccggtgaag aagagatgag
    13981 ctttactctg cagccggtga ccagcaatga gattgacagc gatgcgctgt atctcgatga
    14041 aattacgctt accagcgagc aggatgcagt tctgcgctat ggtcaggtgg aagtaccgct
    14101 cccgccggga gccgacgttg agcgcacaac atggggcatt tcagtcaata aacccaacgc
    14161 cgcgaaacag caggggcaat tgctggaaaa agcgcgtaat gaaatgggcg aactggccta
    14221 tatggtgccg gtgaaagaac tgacgggagc ggtcactttc cgccatttgc tgcgcttctc
    14281 gcaaaaaggg caattcgttc tgcctcctgc tcgttatgtg cgttcctatg cacctgcgca
    14341 gcaaagtgtt gcggcaggga gtgaatggac cgggatgcag gtgaaataag tgaactggcg
    14401 cagaatcgtc tggctattgg cgttggttac tctgccaaca cttgcggagg agacgccgtt
    14461 acaactggtg ctgcgcggtg cgcagcacga tcaactgtat cagctgtcgt catcaggcgt
    14521 gaccaaagtc tcagcattac ctgactcgct caccacgccg ttagggagtt tgtggaaact
    14581 gtatgtttac gcctggctgg aggataccca tcaaccggag cagccctacc agtgtcgcgg
    14641 aaactccccg gaagaggttt attgttgtca ggctggggag agcataacgc gcgataccgc
    14701 gctggtacgt tcctgtgggc tctattttgc gccgcagcgg ttgcatattg gcgcagatgt
    14761 gtgggggcaa tactggcaac agcgtcaggc acctgcgtgg ttagcgtctc tgactacgct
    14821 gaagccagaa acctcggtaa cggttaaatc gctgctcgat tcattagcga cgttacctgc
    14881 gcaaaacaaa gctcaggaag tcttactgga tgtggtgctg gacgaggcga aaatcggtgt
    14941 cgcatccatg ctcggtagtc gggtacgggt gaaaacctgg agctggtttg ccgatgataa
    15001 acaggaaatc cgtcagggtg gttttgccgg ctggttaacc gatggtacac cgctgtgggt
    15061 tactggcagt ggcaccagca aaacggtact aatccgttat gcgacggtgc ttaatcgggt
    15121 tttgccagtg ccgacgcagg ttgccagtgg gcaatgtgta gaggtggagc tgtttgcccg
    15181 ctatccgctg aagaaaatca cagcggaaaa gagtacgacg gcagtaaaac ccggcgtact
    15241 taatgggcgc tatcgtgtga cgtttactaa tggtaaccac attacgtttg tcagccacgg
    15301 tgagacgacc ttactgagcg aaaagggcaa actgaaattg cagtcgcatc tcgatcgtga
    15361 agagtacgtt gccagagttc tggatcgcga ggcgaaaagt acgccccctg aggcagcgaa
    15421 agccatgacc gtggcgattc gcactttttt acagcaaaac gccaatcggg aaggcgattg
    15481 cctgaccatc cccgacagca gcgccacgca gcgtgtttct gcttcgccag cgaccacggg
    15541 ggcgcgaacg atgaccgcct ggacgcagga tctcatctat gcaggcgatc cggttcatta
    15601 tcatggcagt cgcgctaccg aagggacact ttcctggcgg caagccacgg cccaggcggg
    15661 gcagggggag cgttacgacc agatactcgc gtttgcttat cccgacaaca gccttagccg
    15721 ctggggagct ccacgctcaa cctgccagtt attacccaaa gcgaaagcct ggctggcgaa
    15781 aaaaatgccg cagtggcggc gtatattaca aggcgagacg gggtacaacg aaccagacgt
    15841 gtttgcggtc tgtcgtttag tctcaggttt cccctatacc gatcggcagc agaaacggct
    15901 gtttattcgt aacttcttta cgcttcagga tcggctggat ttaacccatg agtatctgca
    15961 cctggccttt gatggttatc ccaccgggct tgatgagaac tatatcgaaa cgctgacccg
    16021 acaattatta atggactaat gctatgcgaa agatttttct tccgttgtta ctggtggcac
    16081 tttcgcctgt cgctcacagt gaaggtgtgc aggaagtcga gattgacgca ccgctctctg
    16141 gctggcatcc ggcagagggc gaagatgcca gtttttcaca atcgatcaat tacccggcgt
    16201 cgtccgtcaa catggcagat gatcaaaata tctcagcgca gatccgcggc aaaataaaga
    16261 attatgctgc ggcgggtaaa gttcagcagg gccggctggt ggtcaacggt gccagtatgc
    16321 cgcagagagt tgaatccgat ggttcatttg cacgccctta tattttcact gaaggcagca
    16381 acagcgtgca ggtcatcagc ccggatgggc aaagccgaca aaaaatgcag ttttactcaa
    16441 cgccgggtac aggaacgatt cgtgcacgtt tacggctggt tctctcgtgg gatacggaca
    16501 ataccgatct cgatctccat gttgtcacgc ccgatggcga acacgcctgg tacggtaaca
    16561 ccgtgctgaa aaacagtggt gcactggata tggatgtcac gacggggtac ggacccgaga
    16621 ttttcgccat gccagcacca gttcacgggc gctatcaggt gtatatcaac tactatggtg
    16681 ggcgcagcga aacggaattg acgaccgccc aactgacgct gatcaccgat gaaggatcgg
    16741 tcaatgagaa acaggaaaca tttattgtac cgatgcgtaa tgctggcgag ctgacgctgg
    16801 tgaaaagttt tgactggtga tccatccctt cacacaacct ggctctctct tttttcagat
    16861 aagactgctg ggcgatctgc cgacgccagg atataaccat taacaagcgt cggcagattg
    16921 gtcatttctt atttttcgta ctgagaactg tccatctact ccggctttcc tggttaaggc
    16981 aatggtttct aagcgatacg cattattgca atggtgacag aacattgcgg taacacgctt
    17041 ttaccgctac cttaaccaca ctccatcggt cacctgaggc ggagcttcgc ccctttgaaa
    17101 taccttgctt cttttcgtac aaccctgaaa gcctcgcgct acatgttcag agcattggcg
    17161 ttagtgctct ggctgttgat tgctttttca tccgttttct acatcgttaa tgcgttacat
    17221 cagcgagaat cggaaattcg tcaggaattt aatctgagtt ccgatcaggc tcagcgcttt
    17281 attcaacgca cctctgatgt gatgaaagag ctgaagtaca tcgccgaaaa tcgcttatcg
    17341 gcagaaaacg gtgtgctttc cccgcgtgga cgagaaacgc aggcggatgt gcctgcgttt
    17401 gaaccgctgt ttgccgactc cgattgttcc gcaatgagta acacctggcg aggttctctg
    17461 gagtcattgg cgtggtttat gcgctactgg cgcgataatt tttctgcggc ttacgatctc
    17521 aaccgggtat ttttaatcgg cagtgataac ctctgcatgg ccaatttcgg tctgcgtgat
    17581 atgccagtgg aacgcgatac cgcgttgaaa gctttgcatg aacgcatcaa taaatatcga
    17641 aatgcaccac aagatgatag cggcagtaac ctctactgga tcagcgaagg tccgcgccct
    17701 ggcgtcgggt atttttacgc gttgacgcca gtttatctgg cgaaccggtt gcaggcgctt
    17761 ttgggtgtcg agcagaccat ccggatggag aactttttct taccgggtac gttgccgatg
    17821 ggggttacca ttcttgatga aaatggtcat accctgattt cgcttaccgg accagaaagt
    17881 aaaattaagg gcgatcctcg ctggatgcag gaacgctcct ggtttggcta tacggaaggg
    17941 ttccgggagc tggtgctgaa gaaaaatctg ccaccctcat cgctaagcat cgtgtattcg
    18001 gtgccggttg ataaggtgct ggaacgcatt cgcatgttga tccttaacgc aattttgctg
    18061 aatgtgcttg ccggagctgc attgtttact ctcgcgcgga tgtacgagcg acgtattttc
    18121 attccggcgg aaagcgacgc cctgcgactg gaagaacatg agcagttcaa tcgcaagatt
    18181 gtcgcctccg cgccagtggg tatctgtatt ttgcgtaccg ctgatggcgt caatatttta
    18241 agtaacgaac tggcgcatac ctatctcaat atgcttacgc atgaggaccg ccaacgactg
    18301 acgcaaatta tctgtgggca gcaggtcaat tttgttgatg tcctgaccag caacaatacc
    18361 aatctgcaaa tcagcttcgt ccattcgcgc tatcgtaatg aaaacgtggc catttgtgtg
    18421 ctggtggatg tttcttcgcg cgtgaagatg gaagagtcgt tgcaggagat ggcacaagca
    18481 gcggaacagg cgagccagtc aaaatcgatg ttccttgcca ccgtcagtca tgagctgcga
    18541 acgccgctgt atggcattat cggtaacctg gatctgttgc aaaccaaaga gttaccgaaa
    18601 ggcgtcgatc ggctggtgac ggcaatgaac aactcttcca gcctgttgtt gaaaattatc
    18661 agcgatattc tcgatttctc gaagattgaa tcggaacagt tgaagatcga accgcgtgag
    18721 ttttcaccgc gtgaagtgat gaaccacatc accgccaact atttaccgct ggtggtacgc
    18781 aagcagttag gcttgtactg ctttattgaa ccggatgtgc cagtggcctt aaatggcgac
    18841 ccgatgcgtt tacagcaggt catctccaac ctgttgagta acgccataaa attcaccgat
    18901 accggctgta tagttttgca tgtccgcgct gatggcgatt atctctctat ccgtgttcgc
    18961 gataccggcg tggggattcc ggcgaaagaa gtggtgcgct tgtttgatcc cttcttccag
    19021 gtcggaacgg gcgtacagcg aaacttccag gggaccggtc tgggtctggc gatttgtgaa
    19081 aaactgatca gcatgatgga cggcgatatc tcggtagatt cagaaccggg aatgggcagc
    19141 cagtttaccg tgcgtattcc gttgtacggc gctcagtacc cgcagaaaaa aggcgtggaa
    19201 gggttgagtg gtaaacgctg ctggctggcg gtccgcaatg cgtcgctctg tcagttcctg
    19261 gaaaccagtt tgcagcgcag cggcatcgtc gttacaacat acgaagggca ggaaccgact
    19321 cccgaagatg tgttaattac tgacgaggta gtgagtaaaa aatggcaggg tagagcggta
    19381 gtgaccttct gtcgtcgcca tattggtatt ccgctggaga aagcgccagg ggagtgggta
    19441 cacagtgtgg ctgccccgca tgagctaccg gcattgttgg cgcgtattta tttgatcgag
    19501 atggagagcg acgatcctgc taacgctctg ccgtcgacgg acaaagcggt cagcgataat
    19561 gacgatatga tgattctggt cgtggatgat catccgatta accgacgtct gctggcagat
    19621 cagttgggat cgttgggcta tcaatgtaaa accgcgaatg atggtgtcga tgcgcttaat
    19681 gtacttagca agaatcatat tgatatcgtg cttagcgacg tcaacatgcc aaatatggat
    19741 ggttaccgct tgacgcaacg cattcgtcag ttgggactga cgttgccggt aatcggagta
    19801 actgctaatg cgttggctga agagaagcag cggtgtctgg agtccggtat ggacagctgc
    19861 ctgtcgaagc cggtaacgct ggatgtgata aaacagacgc tgacggtata tgccgagagg
    19921 gtcaggaaat cgcgggaatc gtaggacgga taaggtgttt atgccgcatc tggcattcag
    19981 tgcaaatgcc agatgcgacg ctgacgcgtc ttatctggcc tactttaatg taatctgttg
    20041 aaataatggg aatcgtaggc cggataaggc gtttacgctg catccggcaa tcggtgcaaa
    20101 tgccagataa gacactaacg cgtcttatct ggcctacaga tgattagtct ttatctgccg
    20161 gacttaaggt cacagaagag agataattca gcagggcgat atcgttctcg acacccagct
    20221 tcatcatcgc agatttcttc tggctactga tggttttaat actgcggttc agctttttag
    20281 cgatctcggt caccaggaag ccttccgcaa acaggcgcag aacttcactc tcttttggcg
    20341 agagacgctt gtcaccgtaa ccaccagcac tgattttttc caacaggcga gaaacgcttt
    20401 ccggggtaaa tttcttccct ttctgcagcg cggcgagagc tttcggcaga tcggtcggtg
    20461 caccttgttt cagcacgatc ccttcgatat ccagatccaa taccgcacta agaatcgccg
    20521 ggttgttgtt catggtcaga acaatgatcg acaggcttgg gaaatggcgc ttgatgtact
    20581 tgattaaggt aatgccatcg ccgtacttat cgccaggcat ggagagatcg gtaatcaaca
    20641 catgcgcatc cagtttcggc aggttgttga tcagtgctgt agagtcttca aattcgccga
    20701 caacattcac ccactcaatt tgctcaagtg atttgcgaat accgaacaag actatcggat
    20761 ggtcatcggc aataattacg ttcatattgt tcatgtaata ggctaccttg ctacagcaag
    20821 ctcttgacat aactgtcaat gtcgctgatg tatttttcta ttcctggaac atccttctca
    20881 cgaatcagat gttccagcgt ttcacataac tgcttgccgg gtaccagatt tagcatggca
    20941 aatacgcctt taagacgatg agccgtttgg gctaacgcag caaagtcact ggttgctgct
    21001 tcagtataca gcctcttaac atcatccggt actgtgtcta caaagagcgc ataatagccg
    21061 ctggcatgga gttgcgcatt ttcatctccg cccagaggcg attctgtcac ctcttcctgc
    21121 gccagttgca cttcaattaa ttgtaagacc gcttcctgca tagcgttgct catattgaag
    21181 ttgacgcaca attgaccagg cccaatttcc cgtacgccag actcatcatc gcttaaaagc
    21241 aagccagagg cagtaagatt agacggatta tccgttaaaa agatatcata atcttgacta
    21301 attaatcttt catcgggtgt gatacaggtt gcaccccaat tttctaactg gcgagtgaca
    21361 atattccgaa tttctgccga agtaacatcc accattacgc agacatcatc cagtaaacgc
    21421 tcttcttcct cttcaacttc cgggtcagct gcgagcattt tgatatgcac agagtagcgt
    21481 gtaccaagcc catcccgcgt tttgatgttt aaatgaccgc ccagtttacg tgccagttga
    21541 tcgctcagcc agaatgccag cgggtccgcc ttgccatagc gatcgttttg ggtctggttg
    21601 ataaacggga agtgcaaatt atccatttca tgaatactta cgccttctcc cgtgtccaga
    21661 atgcggaacg tcaggcggtc ttcggaggac tcatcctgat caacctcaag ggtgattttt
    21721 cccaattgcg ttgaggtcac ggcatattgc atcagtagca gcaaaatacg tcgtaaggca
    21781 tcgcgatcgc cgcggcgcat atcgtgtgct ttcagatgat tgttaatcag cagttgcaga
    21841 cctttacgct tgatggcagg caacactgaa ggcacaactt catcaattaa atcctgcacg
    21901 gagaacagca ccgtctcact tttccagcta tcatccgcaa gcatgttcgc taactgtatt
    21961 tcatcgacca gccgcaccag cacatctgcc tgattcgcca gttgtttgct ttccggggcg
    22021 ttgagtttag ccgcgctctc cgccagggac tgtgcgggtt ctttcagcgc atcgccaatg
    22081 tttttcataa aggtcatccg gccctgctgg tttttctcat acagacgctg cgcctgcttg
    22141 agtttcttgt ttaccagcac ttcgcgatcc tgatcgcgaa taatgaaaat ttgtgtgcgc
    22201 ggcgcgacct ggctgcggaa catgcggatc tcatacagct cgttattgat cgtcgcctga
    22261 ataatcccct gatgctgttc cgccatggtg gtgatgtttt gcagattcaa atgcggcagc
    22321 aaatgatcgg caattttgtt acttatgaca gtgcggttcg attcctgatc gtgaaccagc
    22381 aggccgagcg gcagcagtga gactatctct tcattgattg cccgtaaaat gcgcaattcg
    22441 ttattgaccg ccgtgttggg tagactttct gtactgcgac tggagaaatg gcggaatgtg
    22501 gtatagccaa ataacgccag cgccagcaaa ccgatgttca gcagcagtgg cagcagaatg
    22561 ttttgcaacg tatccagcaa taaggtgcca taaggaacct gccagaccag gcgcatatcg
    22621 gtagagttga gtgccgagga gatttcaatc ttcgtactgt taaagtggat actgacgcta
    22681 tccgttcctt ctttctcatt atcattgtct cccgttgccg tcgtgtctgg ctcaaggcgg
    22741 aaactgtcca gcggcatacc cggtgggatc aaatcattaa tcggcagatc aaaagccacg
    22801 accgttgcca gatgtcccgg ctggttgaac gtggtacgca aggtaaagta atgaccgttc
    22861 tgccaggcca ggcggcgcat gttagaaaaa ctttcgcgtt catcgagggc gttggcctgt
    22921 tgcaacattt ctgcacgacg tgaatcaaca atgtcactga cggtcgattc tttaaatccg
    22981 gaggtgagat ctttgagggg cagggttgag atcagcacca gactgttatc ctgaccattc
    23041 aggtaataca tcgaccacgg tacattttct gcgccccaca atgtgtccag ataggtggac
    23101 atccgttgag tcatctcaag cgttgagttg tcgtgagagc caaagattaa cgcttccgtt
    23161 ttgcggcgtg gtttctccag atagtagaca tcctgtttca ggcgcgtctc ttgtaaacct
    23221 tcgccggagg agggggaggt cgtcgcggca atgttgtcgt agatctgcca ggtcacgtaa
    23281 cgccaggtat cgacgcgctt ttggattgcg tgggtaatgt cgacaatctg gtaactttta
    23341 tctttcagcc atgcgttaac ggcgctttgt accattaccc ccatcgtcac cagtaacaca
    23401 atgatcaaca gtaaaaagaa gcgggtaatg ctccccggta ggagtgaaaa gcgggtcgtg
    23461 gccgttgtct ctttctgacg cattcgtgtt tatgacctgt taaaacttcg cgaagtgata
    23521 gggcagtata aagggtacag tgtgaaattc cagactctta cctgattgat gattctctat
    23581 tctcccgtga tatttatcat cctgtatggg ggaaatagat gtacaagcgc cattttgctg
    23641 tacaaataac ctacaaaaag cccaacgaaa ttaattgcaa ataaacgaag attgctggaa
    23701 attatgctga tgttaattat ttgtgaaata gttaacaagc gttatagttt ttctgtggta
    23761 gcacagaata atgaaaagtg tgtaaagaag ggtaaaaaaa accgaatgcg aggcatccgg
    23821 ttgaaatagg ggtaaacaga cattcagaaa tgaatgacgg taataaataa agttaatgat
    23881 gatagcggga gttattctag ttgcgagtga aggttttgtt ttgacattca gtgctgtcaa
    23941 atacttaaga ataag
//
LOCUS       DAPHDW010000062        23908 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.062, whole genome shotgun sequence.
ACCESSION   DAPHDW010000062 DAPHDW010000000
VERSION     DAPHDW010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23908)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23908)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23908
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.062"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            258..1673
                     /gene="tnaA"
                     /locus_tag="RH057_002445"
     CDS             258..1673
                     /gene="tnaA"
                     /locus_tag="RH057_002445"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HDV3128800.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1764..3011
                     /gene="tnaB"
                     /locus_tag="RH057_002446"
     CDS             1764..3011
                     /gene="tnaB"
                     /locus_tag="RH057_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HDV3128801.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNVDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3143..4318
                     /gene="mdtL"
                     /locus_tag="RH057_002447"
     CDS             3143..4318
                     /gene="mdtL"
                     /locus_tag="RH057_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HDV3128802.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTVSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4293..5252
                     /gene="yidZ"
                     /locus_tag="RH057_002448"
     CDS             4293..5252
                     /gene="yidZ"
                     /locus_tag="RH057_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HDV3128803.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTPLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTWAL
                     DNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQYYNQLHQLPLVALPL
                     PFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA"
     gene            5409..6158
                     /locus_tag="RH057_002449"
     CDS             5409..6158
                     /locus_tag="RH057_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HDV3128804.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPTIEKLSVWEFDGKHGWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKAFSPN"
     gene            6180..6746
                     /gene="yieF"
                     /locus_tag="RH057_002450"
                     /gene_synonym="chrR"
     CDS             6180..6746
                     /gene="yieF"
                     /locus_tag="RH057_002450"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HDV3128805.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQADGVVIVTPEYNYSVPGGLKNAIDWLSRLP
                     DQPLAGKPVLIQTSSMGVIGGARCQYHLRQILVFLDAMVMNKPEFMGGVIQNKVDPQT
                     GEVIDQGTLDHLTGQLTAFGEFIQRVKI"
     gene            complement(6800..8137)
                     /gene="adeP"
                     /locus_tag="RH057_002451"
     CDS             complement(6800..8137)
                     /gene="adeP"
                     /locus_tag="RH057_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HDV3128806.1"
                     /translation="MSQQHTTQASGQGMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQVGMGAIFWGAIGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANPETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPGYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALMFILKIVFIDAH"
     gene            8304..8969
                     /gene="yieH"
                     /locus_tag="RH057_002452"
     CDS             8304..8969
                     /gene="yieH"
                     /locus_tag="RH057_002452"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HDV3128807.1"
                     /translation="MSQIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     RFKGVKLYEIIDIVSLEHGVTLAKTEAEHVYRAEVARLFDSELEAIEGAGALLSAITA
                     PMCVVSNGPNNKMQHSMGKLNMLHYFPDKLFSGYDIQRWKPDPALMFHAAKAMNVNVE
                     NCILVDDSVAGAQSGIDAGMEVFYFCADPHNKPIVHPKVTTFTHLSQLPELWKARGWD
                     ITA"
     gene            9036..9509
                     /locus_tag="RH057_002453"
     CDS             9036..9509
                     /locus_tag="RH057_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CbrB"
                     /protein_id="HDV3128808.1"
                     /translation="MSVSRRVIHHGLYFAVLGPLIGVLFLVLYIFFAKEPLVLLVIIQ
                     VLPLFLLLSITTGAIPALLTGVMVACLPEKIGSQKNYRCLAGGIGGVVITEIYCAVIV
                     HIKGMASSELFENILSGDSLVVRIIPALLAGVVMSRIITRLPGLDISCPETDSLS"
     gene            9558..10145
                     /locus_tag="RH057_002454"
     CDS             9558..10145
                     /locus_tag="RH057_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CbrC family protein"
                     /protein_id="HDV3128809.1"
                     /translation="MTQNIRPLPQFKYHPKPLETGAFEQDKTVECDCCEQQTSVYYSG
                     PFYCVDEVEHLCPWCIADGSAAEKFAGSFQDDASIEGVEFEYDEEDEFAGIKNTYPDE
                     MLKELVERTPGYHGWQQEFWLAHCGDFCAFIGYVGWNDIKDRLDEFANLEEDCENFGI
                     RNSDLAKCLQKRGDCQGYLFRCLHCGKLRLWGDFS"
     gene            complement(10207..10929)
                     /locus_tag="RH057_002455"
     CDS             complement(10207..10929)
                     /locus_tag="RH057_002455"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV3128810.1"
                     /translation="MKLIITEDYQEMSRVAAHHLLGYMSKTRRVNLAITAGSTPKGMY
                     EYLTTLVKGKPWYDNCYFYNFDEIPFRGKEGEGVTITNLRNLFFTPAGIKEENIQKLT
                     IDNYREHDQKLAREGGLDLVVLGLGADGHFCGNLPNTTHFHEQTVEFPIQGEMVDIVA
                     HGELGGDFSLVPDSYVTMGPKSIMAAKNLLIIVSGASKAQALKNVLQGPVTEDVPASV
                     LQLHPSLMVIADKAAAAELALG"
     gene            complement(10944..12113)
                     /locus_tag="RH057_002456"
     CDS             complement(10944..12113)
                     /locus_tag="RH057_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HDV3128811.1"
                     /translation="MNIKIAALTLAIASGISAQWAIAADMPASPAPTIPVKQYVTQVN
                     ADNSVTFRYFAPGAKNVSVVVGVPVPDNIHPMTKDEAGVWSWRTPILKGNLYEYFFNV
                     DGVRSIDTGTAMTKPQRQVNSSMILVPGSYLDTRSVAHGDLIAITYHSNALQSERQMY
                     VWTPPGYTGMGEPLPVLYFYHGFGDTGRSAIDQGRIPQIMDNLLAEGKIKPMLVVIPD
                     TETDAKGIIPEDFVPQERRKVFYPLNAKAADRELMNDIIPLISKRFNVRKDADGRALA
                     GLSQGGYQALVSGMNHLESFGWLATFSGVTTTTVPDECVAARLNDPAAINQQLRNFTV
                     VVGDKDVVTGKDIAGLKTELEQKKIKFDYQEYPGLNHEMDVWRPAYAAFVQKLFK"
     gene            complement(12140..13756)
                     /gene="bglH"
                     /locus_tag="RH057_002457"
     CDS             complement(12140..13756)
                     /gene="bglH"
                     /locus_tag="RH057_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate-specific porin BglH"
                     /protein_id="HDV3128812.1"
                     /translation="MFRRNLITSAILLMAPLAFSAQSLAESLTVEHRLELLEKALRET
                     QSELKKYKDEEKKKYTPATVNRSVSTNDQGYAANPFPTSSAAKPDAVLVKNEEKNASE
                     TGSIYSSMTLKDFSKFVKDEIGFSYNGYYRSGWGTASHGSPKSWAIGSLGRFGNEYSG
                     WFDLQLKQRVYNENGKRVDAVVMMDGNVGQQYSTGWFGDNAGGENFMQFSDMYVTTKG
                     FLPFAPEADFWVGKHGAPKIEIQMLDWKTQRTDAAAGVGLENWKVGPGKIDIALVRED
                     VDDYDRSLQNKQQINTNTIDLRYKDIPLWDKATLMVSGRYVTANESASEKDNQDNNGY
                     YDWKDTWMFGTSLTQKFDKGGFNEFSFLVANNSIASNFGRYAGASPFTTFNGRYYGDH
                     TGGTAVRLTSQGEAYIGDHFIVANAIVYSFGNDIYSYETGAHSDFESIRAVVRPAYIW
                     DQYNQTGVELGYFTQQNKDANSNKFNESGYKTTLFHTFKVNTSMLTSRPEIRFYATYI
                     KALENELDGFTFEDNKDDQFAVGAQAEIWW"
     gene            complement(13842..15236)
                     /gene="bglB"
                     /locus_tag="RH057_002458"
     CDS             complement(13842..15236)
                     /gene="bglB"
                     /locus_tag="RH057_002458"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HDV3128813.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWQEDGKGISTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYHRYPEDIALFAEMGFTCLRISIAWARIFPQGDEVEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMPYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHEPFTGVGLAEESGEAEVYQAIHHQLVASARAVKACHSLLP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDSVGLRVLLNTLWDRYQKPLFIVENGLGAKDSVEADGSIQDDYRIAYLNDHLVQV
                     NEAIADGVDIMGYTSWGPIDLVSASHSQMSKLYGFIYVDRDDNGEGSLTRTRKKSFGW
                     YAEVIKTRGLSLKK"
     gene            complement(15255..17132)
                     /gene="bglF"
                     /locus_tag="RH057_002459"
     CDS             complement(15255..17132)
                     /gene="bglF"
                     /locus_tag="RH057_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HDV3128814.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     NTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFWFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADELGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGPLSTWISELIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHWGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTVMLH
                     FITAKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVELLIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(17265..18101)
                     /gene="bglG"
                     /locus_tag="RH057_002460"
     CDS             complement(17265..18101)
                     /gene="bglG"
                     /locus_tag="RH057_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HDV3128815.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRPGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRIISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLPNPLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMH
                     LVSAQMSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILE
                     HASINDSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRK
                     EH"
     gene            complement(18387..19112)
                     /gene="phoU"
                     /locus_tag="RH057_002461"
     CDS             complement(18387..19112)
                     /gene="phoU"
                     /locus_tag="RH057_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HDV3128816.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAEKDSDK"
     gene            complement(19127..19900)
                     /gene="pstB"
                     /locus_tag="RH057_002462"
     CDS             complement(19127..19900)
                     /gene="pstB"
                     /locus_tag="RH057_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HDV3128817.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(19991..20881)
                     /gene="pstA"
                     /locus_tag="RH057_002463"
     CDS             complement(19991..20881)
                     /gene="pstA"
                     /locus_tag="RH057_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HDV3128818.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(20881..21840)
                     /gene="pstC"
                     /locus_tag="RH057_002464"
     CDS             complement(20881..21840)
                     /gene="pstC"
                     /locus_tag="RH057_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HDV3128819.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(21927..22967)
                     /gene="pstS"
                     /locus_tag="RH057_002465"
     CDS             complement(21927..22967)
                     /gene="pstS"
                     /locus_tag="RH057_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HDV3128820.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(23215..>23908)
                     /locus_tag="RH057_002466"
     CDS             complement(23215..>23908)
                     /locus_tag="RH057_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3128821.1"
                     /translation="NAPFEYHANNPSGNTKYNCNRIEPLSISSGAKAIVYFYIKKTFA
                     GKLIIPETKIVTLYGTISRDTPVDYSQPMADVYIRGDISAPQSCEINNLQPVYFDFKE
                     IPAADFSSVVGSAVTTHKITKTVTIECENLGILNTDDISTSFYATEPNTDNSMVVTSN
                     SNVGIKIYDKNNKEIKVNGGELPTDMGKSTVYGEKSGSVTFSAAPASLTGARPAPGQF
                     TATATITVEIVR"
BASE COUNT         6033 a         6024 c         5676 g         6175 t
ORIGIN      
        1 caatatcgac aataagattg tcgatcaccg cccttaattt acccttctgt agccatcacc
       61 agagccaaac cgattagatt caatgtgaac gattagtttg ctatatctta attttgcctt
      121 ttgcaaaggc catctctcgt ttatttactt attttagtaa atgatggtgc ttgtatatat
      181 acatggcgaa ttaatggata ttgcagatgt aatattcaca gggatcactg taattaaaat
      241 aaatgaagga ttatgtaatg gaaaacttta aacatctccc tgaaccgttc cgcattcgtg
      301 ttattgagcc agtaaaacgt actactcgcg cttatcgtga agaagcaatt attaaatccg
      361 gtatgaaccc gttcctgctg gatagcgaag atgtgtttat cgatttactg accgacagcg
      421 gcaccggggc ggtaacccaa agtatgcagg cagcgatgat gcgcggcgac gaagcctaca
      481 gcggcagccg cagctactat gcgttagccg agtcagtgaa aaatatcttt ggttatcaat
      541 acaccattcc gactcaccag ggccgtggcg cagagcaaat ttatattccg gtactgatta
      601 aaaaacgcga gcaggaaaaa ggcctggatc gcagcaaaat ggtggcattc tctaactatt
      661 tctttgatac cacgcagggc catagccaga ttaacggctg taccgtgcgt aacgtctata
      721 tcaaagaagc cttcgatact ggcgtgcgtt acgactttaa aggtaacttt gaccttgagg
      781 gattagaacg cggtattgaa gaagttggcc cgaataacgt gccgtatatc gttgcaacca
      841 tcaccagtaa ctctgcaggt ggtcagccgg tttcactggc aaacttaaaa gcgatgtaca
      901 gcatcgcgaa gaaatacgat attccggtgg taatggactc cgcacgcttt gctgaaaacg
      961 cctatttcat caagcagcgt gaagcagaat acaaagactg gaccatcgag cagatcaccc
     1021 gcgaaaccta caaatatgcc gatatgctgg cgatgtccgc caagaaagat gcgatggtac
     1081 cgatgggcgg cttgctgtgc atgaaagacg acagcttctt tgatgtgtac accgagtgca
     1141 gaaccctttg cgtggtgcag gaagggttcc cgacatatgg cggcctggaa ggcggcgcga
     1201 tggagcgtct ggcggtaggt ctgtatgacg gcatgaatct cgactggctg gcttatcgta
     1261 tcgcgcaggt acagtatctg gtcgatggtc tggaagagat tggcgttgtc tgccagcagg
     1321 cgggcggtca cgcggcattc gttgatgccg gtaaactgct gccgcatatc ccggcagacc
     1381 agttcccggc acaggcgctg gcgtgcgagc tgtataaagt cgccggtatc cgtgcggtag
     1441 aaattggctc tttcctgtta ggccgcgatc cgaaaaccgg taaacaactg ccatgcccgg
     1501 ctgaactgct gcgtttaacc attccgcgcg caacatatac tcaaacacat atggacttca
     1561 ttattgaagc ctttaaacat gtgaaagaga acgcggcgaa tattaaagga ttaaccttta
     1621 cctacgaacc aaaagtattg cgtcacttca ccgcaaaact gaaagaagtt taattaatac
     1681 tacagagtgg ctataaggat gttagccact ctcttaccct acatcctcaa taacaaaaat
     1741 agccttcctc taaaggtggc atcatgactg atcaagctga aaaaaagcac tctgcatttt
     1801 ggggtgttat ggttatagca ggtacagtaa ttggtggagg tatgtttgct ttacctgttg
     1861 atcttgccgg tgcctggttt ttctggggtg cctttatcct tatcattgcc tggttttcaa
     1921 tgcttcattc cgggttattg ttattagaag caaatttaaa ttatcctgtc ggctccagtt
     1981 ttaacaccat caccaaagat ttaatcggta acacctggaa cattatcagc ggtattaccg
     2041 ttgccttcgt tctctatatc ctcacttatg cctatatctc tgctaatggt gcgatcatta
     2101 gtgaaacgat atcaatgaat ttgggctatc acgctaatcc acgtattgtc gggatctgca
     2161 cagccatttt cgttgccagc gtattgtgga taagttcgtt agccgccagt cgcattacct
     2221 cattgttcct cgggctgaag attatctcct ttgtgatcgt gtttggttct ttcttcttcc
     2281 aggtcgatta ctccatcctg cgcgatgcca ccagcaccac tgcgggaacg tcttacttcc
     2341 cgtatatctt tatggctttg ccggtgtgtc tggcgtcatt tggtttccac ggcaatattc
     2401 ccagcctgat tatttgctat ggaaaacgca aagataagtt aatcaaaagc gtggtatttg
     2461 gttcgctgct ggcgctggtg atttatctct tctggctcta ttgcacgatg gggaatattc
     2521 cgcgcgaaag ctttaaggcg ataatctcct caggcggcaa cgttgattcg ctggtgaaat
     2581 cgttcctcgg caccaaacag cacggcatta tcgagttttg cctgctggtg ttctctaact
     2641 tagctgttgc cagttcgttc tttggtgtca cgctggggtt gttcgattat ctggcggacc
     2701 tgtttaagat tgataactcc cacggcgggc gtttcaaaac cgtgctgtta accttcctgc
     2761 cacctgcgtt gttgtatctg atcttcccga acggctttat ttacgggatc ggcggtgccg
     2821 ggctgtgcgc caccatctgg gcggtcatta ttcccgcagt gcttgcaatc aaagctcgca
     2881 agaagtttcc caatcagatg ttcacggtct ggggcggcaa tcttattccg gcgattgtca
     2941 ttctctttgg tataaccgtg attttgtgct ggttcggcaa cgtctttaac gtgttaccta
     3001 aatttggcta aatccttcaa gaagccagcc attcgctggc ttcttgcctc tcaggaaatc
     3061 acttatgtcc aaatggcaac tcgcctgatc ctccttcacc acgtatgctt tgcgtcacct
     3121 tactatcagg acgctttagc ccatgtcccg ctttttgatt tgtagttttg ccctggtttt
     3181 actttatccc gccgggattg atatgtacct cgttggttta ccgcgcatcg ccgccgatct
     3241 caatgccagc gaagcgcagt tgcatattgc gttctccgta tatctggcgg ggatggcagc
     3301 tgcgatgtta tttgccggta aagtggccga tcgttcaggg agaaagccgg tcgccatacc
     3361 cggcgcggcg ctatttatta ttgcctcggt gttctgttca ctggctgaaa ccagcacgtt
     3421 atttcttgca ggccgatttc tacaggggtt gggcgcaggc tgttgttacg tagtggcgtt
     3481 cgccattttg cgcgacacgc tggatgatcg acgtcgggct aaagtgctgt cattactcaa
     3541 cggtattacc tgcatcattc cggtgttagc gccagtgctc ggacatctga ttatgcttaa
     3601 attcccgtgg cagagtctgt tctgggcgat ggcaatgatg ggcatcgcgg tactgatgtt
     3661 gtctttgttt attttaaaag aaacgcgccc agcgtccccc gctgcttcgg acaaaccacg
     3721 agaaaatagc gagtcgctgc ttaatcggtt tttcctcagc cgtgttgtta tcaccaccct
     3781 cagcgtttcg gtgatcctca ctttcgtcaa tacatcgccg gtattgctga tggaaatcat
     3841 gggttttgag cgcggagaat acgccaccat tatggcgttg actgctggcg tcagcatgac
     3901 cgtttcattc tccacgccat ttgcgctggg aatttttaag ccacgtacgt tgatgatcac
     3961 ctcgcaggtg ttattccttg cagcggggat cacccttgcc gtttcacctt cccatgcggt
     4021 ttctctgttt ggtatcacgc tgatttgcgc cggtttctcg gtaggttttg gcgtagcgat
     4081 gagtcaggcg ttaggaccat tttcattacg cgcgggcgta gccagctcga ccttaggtat
     4141 tgcgcaggtt tgcggttcgt cactgtggat ttggctggca gcggtggttg gtatcggcgc
     4201 atggaatatg ctgatcggga ttctgattgc ctgtagcata gtgagcctgt tgctgattat
     4261 gttcgtcgcg cctggacgcc ccgttgccgc tcatgaagaa atccatcacc acgcttgatc
     4321 tcaatctgct gctctgtctg caactgctga tgcaggagcg cagcgtgacc aaagcggcga
     4381 agcggatgaa cgtgacccct tcagcggtga gtaagtcgct ggcaaagtta agagcgtggt
     4441 ttgacgaccc gctctttgtg aactcaccgc tgggcctgtc gcccacaccg ctaatggtca
     4501 gcatggagca aaatctggcg gaatggatgc aaatgagcaa tctgctgctg gataaaccgc
     4561 accaccagac accgcgcggc ctgaagtttg agttggcagc ggaatcgccg ctgatgatga
     4621 tcatgcttaa tgcgctgtcg aaacggatct accaacgtta cccgcaggcg accatcaaac
     4681 tacgtaactg ggattacgat tccttagatg ccattactcg tggtgaagtg gatattggtt
     4741 tttccggtcg cgaaagccat cctcgctcgc gggagctgtt aagctcgcta ccgttagcca
     4801 ttgattatga agtgctgttt agtgatgtac cctgcgtctg gttacgcaaa gatcatccgg
     4861 cactgcatga aacgtggaat ctggacacct ttttacgtta tccgcatatc agcatttgct
     4921 gggaacagag cgacacctgg gcgctggaca atgtgttaca ggagctgggg cgcgaacgca
     4981 cgattgctat gagcctgccg gaattcgagc agtcactgtt tatggcagcg caacccgaca
     5041 atctgctact ggcgaccgcg ccgcgctact gtcagtacta caatcaactc catcaactgc
     5101 cgttggttgc tcttcctctc ccgtttgacg aaagccagca aaaaaagctg gaagttcctt
     5161 ttaccctgct gtggcataaa cggaacagcc ataatccgaa gatcgtctgg ttacgggaaa
     5221 ccattaaaaa cctttacgcg tcgatggcat aaccgaatcg tatgaaacgc gacccaattt
     5281 cacaataaaa tgtaaaaaag ttgtaataag cttgtctgaa tcgaactttt agccgcttta
     5341 gtctgtccat cattccagta aatgattact cttgtattca taatggacca ttaagcatgg
     5401 agcgaaaaat ggcgactcac tttgcccgag ggattttaac ggaaggacat ctgatttctg
     5461 ttcgtctccc ctcccagtgt catcaagaag cccgaaacat tccccctcat cgtcaaagcc
     5521 gttttctggc gtctagaggt ttactcgcag aactgatgtt catgctgtat ggcattggcg
     5581 aattgccgga aatcgtcacc ctgccgaaag gtaaaccggt tttcagtgat aaaaatttgc
     5641 cttcgttttc tatttcctat gccgggaata tggttggcgt ggcgttaaca accgaaggtg
     5701 aatgtggtct cgatatggaa ctacagcgtg cgacgcgcgg gtttcatagc ccacacgcgc
     5761 ccgataacca caccttttcc agcaatgaat cgctatggat cagtaaacaa aacgatccta
     5821 acgaagcgcg ggcgcagctc atcacgctgc gccgaagcgt gctaaaacta accggtgatg
     5881 ttttgaatga cgatccgcgc gatctgcagc tgctgcccat tgccggacgc ctgaaatgtg
     5941 ctcatgtaaa tcatgtagaa gcgttatgcg acgcggagga cgtgctggtg tggtccgtgg
     6001 cggtcacgcc cacgattgaa aagctcagtg tctgggagtt cgatggcaaa cacggctgga
     6061 aaagcctgcc ggatattcac agccgcgcca acaatcctac cagccggatg atgcgttttg
     6121 cccagctctc taccgtgaag gctttttcgc caaattgata gacaaccaca ggagtcatca
     6181 tgtctgaaaa attgcaggtg gttacgttac tggggagcct gcgcaaaggc tcatttaatg
     6241 gcatggttgc acgtaccctg ccgaaaattg ctccggcgag catggaagtc aatgcgttac
     6301 cttccattgc cgacattccc ttgtatgacg ctgacgtaca gcaggaagaa ggttttccag
     6361 caacggttga agctctggcg gaacagatcc gtcaggctga cggtgtggtg atcgtcacgc
     6421 cggaatataa ctactcggta ccgggtgggc tgaaaaatgc catcgactgg ctttcccgcc
     6481 tgccggatca accgctggcc ggtaaaccgg tgttgattca gaccagctca atgggcgtga
     6541 ttggcggcgc gcgctgtcag tatcacctgc gccagattct ggttttcctc gatgcaatgg
     6601 tgatgaacaa gccggaattt atgggcggcg tgattcagaa caaagttgat ccgcaaaccg
     6661 gagaagtgat tgatcagggt acgctggacc acctgaccgg gcaattgacc gcatttggtg
     6721 agtttattca gcgagttaag atctaaataa aaaacccgcc agcaatcatg catggcgggt
     6781 ttttaatgcg ctatcgattt taatgagcgt cgataaatac aatcttcagg ataaacatca
     6841 gcgcaacgat gattacgcac gggctaaggt cacgcagacg cccggtacca atcttcatca
     6901 cgcagtagga gataaagccc agcgcaatac cttcggtaat cgaaaagctg aacggcatca
     6961 tcacggcggt aataaacgcc ggaacagatt cagtaagatc ctgccagttc acgcgagcaa
     7021 gacttgaggt catcaacacg ccaacgtaga tcaacgcgcc agctgcagcg tagcctggca
     7081 ccattcccgc cagcggcgac agaaagataa ccagcaggaa cagcagacca acaaccactg
     7141 ccgtcagacc ggtacgaccg ccaaccgata cgccggaaga ggactcaata taagccgtaa
     7201 cggaagaagt accgataaac gaaccggtca cggaagagat actgtcgaca tacagcgcct
     7261 gcttcatgcg cgggaattta cccttctcat ccgccaggcc tgctttatcg gtcacgccaa
     7321 tcagcgtacc ggaggagtca aacaggttga ccagcatgaa agagaaaatc acccctgcca
     7381 gcccgaggtt aaacgacccg gctaaatcta catggcccac gacagtcatt acgctcggcg
     7441 gcgcagaaac gatgccattg tagtggacat cacccagcat ccagcccagc agcgttgtca
     7501 ccacgatgga aaccagcacc gccgcgtgaa tgttgcgcga ggccagaata gcgatgatga
     7561 agaagccgag gatccccagc agcacgctgt gagaagtcag gttaccgatg ctgaccagcg
     7621 tttccgggtt agcgacaatc acacctgcat tcttcagtcc catcatacca atgaacagac
     7681 cgataccgct ggtgatgccc acacgcagac ttaccggaat gttagcaatc atccagtaac
     7741 gaacgcggaa aatcgtcagt aacagcagac caatcgcgcc ccagaagatt gcgcccatcc
     7801 cgacttgcca cggcaagccc atagcctgta caacgacaaa agcgaagaat gcattcaggc
     7861 ccatagcggg tgccagtgca actggcaggt tagcaaacag tcccatcata atactgccga
     7921 atgcagcaat cagacaggta gtaacgaaga cggcgctggt atccatgcca gcaacgccaa
     7981 gaatttgcgg gttaacaaaa acgatgtaaa ccatcgtcag gaaggtggta aaaccggcga
     8041 tcacttcggt ccgtgccgtc gtgccatgtt cgcgcagttt aaacacgcgt tccagcatcc
     8101 cctggccaga agcctgggtt gtgtgttgtt gactcattat ctatttccga acaaggaggg
     8161 aaaatccgtc gctatcgtat accaaaatga gacaatatgg acggctgaag ataacttttt
     8221 ttcattgtat ttgcttatac ggcaacgatt gcgtcatgcg aattggcatg acgaaaacgt
     8281 taaactaacg ataaaggaaa gacatgtccc agatagaagc ggtatttttc gactgcgacg
     8341 gtacgctggt cgacagtgaa gtcatttgct cccgcgcata tgtaacgatg tttcaggaat
     8401 ttggtattac gctcgatcct gaagaggtat tcaaacgttt caaaggtgta aaactgtacg
     8461 aaattatcga tattgtttcc cttgaacatg gtgttacgtt agcgaaaaca gaagctgaac
     8521 acgtttaccg tgcagaagtc gctcggctgt tcgattcaga actggaagcc atcgaagggg
     8581 ctggagcgct cctgtcagcg atcactgcgc caatgtgtgt ggtatctaac ggcccaaata
     8641 acaaaatgca gcattctatg ggcaagctga atatgttgca ctacttcccg gataaactgt
     8701 tcagcggcta cgatattcag cgctggaagc cagacccggc gttaatgttc catgcggcaa
     8761 aagcgatgaa tgtaaatgta gaaaactgca ttctggttga tgactcagtt gccggtgcgc
     8821 aatctggtat cgacgcaggt atggaagtgt tctacttctg cgccgacccg cacaataagc
     8881 cgatcgttca cccgaaagtc accaccttta cccatctttc gcagttaccg gaactgtgga
     8941 aagcgcgtgg ttgggatatt acggcatagt tcttcacact cccttcactt accccgctta
     9001 aattggcgct caaaggtaag taaagggagt ttgatatgtc tgtttcacgt cgggtaatac
     9061 atcacggact ttattttgcg gttttaggac cgttaattgg tgttctgttt cttgtcctct
     9121 acatattctt cgcaaaagaa ccgctggttc ttttggtaat aatacaggta cttccattgt
     9181 ttctcttatt gtcgattact acgggagcta ttcctgcatt gttaaccggc gtgatggtcg
     9241 cctgtctgcc ggaaaagatc gggtcacaga aaaattatcg ttgtctggct ggtggcatag
     9301 gtggcgtcgt tatcaccgag atctattgtg cagttattgt acatattaag ggcatggctt
     9361 cctcggagtt gtttgaaaac attctttctg gtgacagtct cgttgtccgc atcattcctg
     9421 cattgctggc aggtgtggtg atgagcagaa tcattacccg tctgcccgga ttggatattt
     9481 catgtcctga aacagactct ttaagttaag cgggatactt tatctttggg ctactcaaaa
     9541 gcagacagga tgttactatg actcaaaata tcaggccgtt accccaattc aaatatcatc
     9601 ccaagccact ggaaacaggc gcatttgaac aggataaaac cgtagagtgc gattgctgtg
     9661 aacaacagac gtcagtttat tactcgggtc ccttttattg cgttgatgaa gttgaacatc
     9721 tctgtccgtg gtgtattgcg gacggttctg ctgctgaaaa gtttgcaggt agttttcagg
     9781 atgatgccag catagaaggt gttgaatttg agtatgatga agaggacgaa tttgccggta
     9841 ttaagaacac atatcctgat gaaatgctga aagagttggt tgaacgcacg ccaggttatc
     9901 atggatggca acaggaattc tggcttgcgc attgtggcga tttctgtgct tttatcggct
     9961 atgtgggctg gaatgatata aaagatcgcc tcgatgaatt tgccaacctt gaagaagatt
    10021 gtgagaattt cggtattaga aattctgatc tagctaaatg cctgcaaaag cgtggtgatt
    10081 gtcagggtta tctcttccgc tgtctccact gcggcaagct gagactgtgg ggtgattttt
    10141 cgtagttatt taaataatga gaacaggccg gagcgtaatt catacatccg gccttatttc
    10201 ttcagcttaa ccgagcgcta attctgcggc agcggcttta tcagcgatta ccatcagcga
    10261 aggatgcagc tgcaacacgg aagctggaac atcttcggta accggacctt gcagcacatt
    10321 tttcagtgcc tgcgctttac ttgcgccact gacaattatc agcaagtttt tcgccgccat
    10381 gatgcttttc ggccccatcg tcacgtagct gtcaggcact aacgaaaagt cgccgcccaa
    10441 ctcaccatgt gccacaatat caaccatctc tccctggatc gggaactcca cggtttgctc
    10501 atggaaatgg gtcgtattcg gcaaattgcc acaaaaatga ccatctgcac ctaatcccag
    10561 caccaccaaa tccaggccgc cttcacgcgc cagtttctga tcatgctcgc ggtagttatc
    10621 aatagtgagc ttctggatat tctcttcttt gatccccgca ggggtgaaaa acagattacg
    10681 cagattggta atcgttacgc cttctccctc tttgccacga aagggaattt catcgaaatt
    10741 atagaaatag cagttatcgt accagggctt acctttaacc agggtggtga ggtattcata
    10801 catgcctttg ggcgtgctac cggcggtaat tgccaggtta acacgacgcg tcttcgacat
    10861 ataaccaagc agatggtgtg ccgcgacacg gctcatttcc tggtaatctt cggtaatgat
    10921 taatttcatc ttaatgcctt attttattta aataatttct gtacgaaggc tgcataggca
    10981 ggccgccaga catccatttc atggttcagg ccgggatatt cctggtaatc aaacttaatt
    11041 tttttctgct caagctcagt tttcagcccg gcgatatcct tgccggttac gacatcttta
    11101 tcaccaacga ccacagtaaa attacgtagt tgctggttaa tagctgccgg atcgttcagc
    11161 cgggccgcga cacattcatc cggtacggtt gtcgtggtaa caccactgaa tgtggccagc
    11221 cagccaaagc tttccagatg attcattccg gaaaccagcg cctggtaccc gccttgtgaa
    11281 agccctgcca gcgcgcgacc atcggcatct ttacggacat taaaacgctt gctaatcagc
    11341 gggataatat cgttcatcag ttcgcgatct gccgctttag cattcagcgg ataaaagact
    11401 ttacgtcttt cctgaggaac gaaatcttcg ggaataatgc ccttcgcatc ggtttcggta
    11461 tccgggatca ccaccagcat cggtttaatt ttcccttcag ccagcaggtt atccatgatt
    11521 tgcgggatac gcccctgatc gatagcggaa cgtccggtat caccaaaacc gtgatagaaa
    11581 tagagcactg gcaaaggctc gcccatgccg gtgtatcctg gcggggtcca gacatacatc
    11641 tgacgttcag attgcaatgc gttggagtgg taagttatgg cgatcaaatc accatgcgca
    11701 acagaacgcg tatccagata actgcctggc accagaatca tactggagtt aacctggcgc
    11761 tgaggcttag tcattgcggt gcctgtatca atgctgcgta caccatcaac attgaaaaaa
    11821 tactcgtaca ggttgccttt caggataggt gtgcgccacg accagactcc tgcttcgtct
    11881 ttggtcatcg ggtgaatatt gtccggaacc ggaacaccca ccactacaga gacatttttt
    11941 gccccagggg caaagtagcg aaaggtcacg ctgttatcgg cattgacctg agtcacatac
    12001 tgtttcaccg ggatagtggg tgccgggctg gctggcatat ccgctgctat ggcccactga
    12061 gcagaaatac cgctggcaat agccagcgtt aaagcagcaa tttttatatt catcgtaata
    12121 ttcctttcat taagttccat taccaccaga tttcagcctg ggcaccgaca gcaaactggt
    12181 cgtctttatt gtcttcgaag gtgaagccat ccagttcgtt ttccagggct ttgatatacg
    12241 tggcgtagaa acgaatttcc ggacgcgagg tcaacatact ggtattgact ttaaaggtat
    12301 ggaagagcgt ggttttataa ccagactcat taaatttatt actattcgca tctttgtttt
    12361 gctgggtgaa atagcccagt tcaacacctg tctggttata ttggtcccaa atataggccg
    12421 ggcgaacaac cgcacgaata gattcgaaat cagagtgtgc gcctgtttcg tagctataaa
    12481 tatcgttacc gaaggagtaa acaatcgcgt tagccacaat aaaatgatcg ccgatatagg
    12541 cttcgccctg cgaggtcaga cgaactgctg ttccgccggt gtgatcacca taataacgac
    12601 cattaaaggt ggtaaatgga cttgcgccag cataacggcc aaagttactg gcgatagagt
    12661 tattcgcaac caggaaggag aattcgttga agccaccttt atcaaatttc tgcgttaaag
    12721 atgtgccgaa catccaggta tctttccagt cataataccc gttattatcc tgattatctt
    12781 tttccgatgc gctttcgttt gccgtgacat aacgaccact caccattaag gtcgctttat
    12841 cccataacgg gatatcttta tagcgtaaat caatagtatt ggtattaatc tgctgtttgt
    12901 tttgcaggct gcgatcgtaa tcatcgacat cttcgcgaac cagcgcgata tcaattttac
    12961 ccggaccgac tttccagttt tccagaccta cacccgctgc ggcatcagta cgctgcgttt
    13021 tccagtccag catctggatt tcaattttcg gcgcaccgtg tttacccacc cagaaatcag
    13081 cctctggcgc aaagggcagg aaacctttgg tggtaacgta catatcggag aactgcataa
    13141 agttctcgcc accggcgtta tcgccaaacc agccggtaga gtactgctga ccaacgttac
    13201 catccatcat cacaacggca tcaacccgtt tgccgttttc gttgtagaca cgttgtttta
    13261 actgcaaatc aaaccagccg gagtattcgt taccaaagcg gcccagagaa ccaatcgccc
    13321 atgatttagg tgaaccatga gaggcggtcc cccaaccaga acggtaatag ccgttgtaac
    13381 taaagccaat ttcatctttc acaaacttac tgaaatcttt cagagtcatg gaagaataaa
    13441 tcgagcctgt ctcactggca tttttctctt catttttgac cagtacagca tcaggttttg
    13501 cggcactact ggtcgggaac ggattggcgg catacccttg atcattcgta cttacgctac
    13561 gattcaccgt cgctggcgta tattttttct tctcttcatc tttatacttt ttgagttcgc
    13621 tttgcgtttc tcttaacgct ttttctaata actcaaggcg atgttccacc gttaatgatt
    13681 cagccaatga ttgtgcagaa aaggctaacg gtgccattaa taagatggca gaggtaataa
    13741 gatttcgtct aaacataata tccctttatg gtgcaaagaa agaattaacg tatcgcatcc
    13801 agactgttct gaatgcgacg ataattaagg tgctttattg gttatttttt taatgacagc
    13861 ccccgcgttt tgatcacttc tgcataccat ccgaagcttt tcttacgcgt tcttgtgagg
    13921 cttccttcgc cattatcatc acgatccaca taaataaagc cgtagagctt agacatttgt
    13981 gaatgagatg cactgactaa atcaattggc ccccaactgg tgtaccccat aatatccaca
    14041 ccatcggcaa tcgcttcatt tacctgtacc aggtgatcgt ttaaataggc aattcgataa
    14101 tcgtcctgta tcgaaccatc cgcttcaacg ctgtcttttg cgcctaatcc gttctcgaca
    14161 ataaataacg gtttttgata acgatcccaa agcgtattta acagaacccg taatccaacc
    14221 gaatcaattt gccaccccca ctctgaactt ttcagatgcg gattggggat catattcagt
    14281 atgttgccct gcgcattttt attaatgctt tcgtcgtggg aaacacaacc agtcatgtaa
    14341 taactaaaag agatgaaatc gacggtatgt tttaaatctt ctgcgtcact ttcagtcatc
    14401 tcaatggtga tattgtggtc gcggaagaaa cgctgcatat agccgggata ctggccacgc
    14461 gcctgaacat caccaaagaa catccagcgc cggttctctt ccatggcctg caacatatcc
    14521 tgtggctggc aggtgagggg gtaaaccagc ccaccgagaa gcatattgcc gattttcgct
    14581 tcggggagca ggctatgaca ggctttaact gcccgcgcac tggcaaccag ttgatggtgg
    14641 atagcctgat aaacttccgc ctcgccactc tcttctgcca gccccacgcc cgtgaatggc
    14701 tcgtgtaacg acatgttgat ttcattaaac gtcagccata acgccacttt atgttggtag
    14761 cgagtaaaga ccgtgcgggc gtaatgctcg aagtgatcga tgaccgctcg attagcccaa
    14821 ccgccgtagt ttttcaccag cccatatggc atttcgtaat gggataacgt taccagcggc
    14881 ttgatccccg cctgcgccat ttcatcaaac agccgatcgt aaaacgctaa ccccgcttca
    14941 ttcggttcga cttcgtcgcc ctgagggaaa attcgcgccc aggcaatgga aatacgcaga
    15001 caggtgaagc ccatctcggc aaataacgcg atatcttccg ggtaacggtg ataaaaatcg
    15061 atggcgacat ctttgatatt ctctttcccc aggatgcgcg gttccatttt tcccattacg
    15121 ccatgaggct gtaaatctga ggtcgagatc cctttgccat cttcctgcca ggcaccttcc
    15181 acctgattgg cagctgttgc gccaccccaa agaaatgttt ctggaaatgc tttcataatt
    15241 aactcctttt atcgttagcg aatgatggat aacagcggtt cacctgcgct tatctgcgcc
    15301 gtgccgtggg gtaatacgtc cgtaaaatca tcgctattac tgattaatac cggcgtcgtc
    15361 agatcaaatc cggcctcgcg aatagcaggg atatcaaaag aaatcagccg atcgcctgta
    15421 ttgaccttgt cacccacgtt gacgtgagcg gaaaagaatt tgccgtccag ttttacggtg
    15481 tcgataccga catgaatcag gagctccaca ccatcatctg actcaatgcc aatggcgtgt
    15541 aatgtggcga acaacgaagc aattcgaccc gcaaccggag aacgcacttc accaaccgag
    15601 ggcagaatgg cgataccttt acccaacagg ccactggcaa acgtggtatc agcgacgtga
    15661 atgagcggca caatctctcc cgtcatcggt gaacagatac cgccctgctc aggtggtgta
    15721 ataacctctg gtgttttctc ttgcggggca ccctgcgctg gctgacgttt agcggtgatg
    15781 aaatgaagca tcaccgtacc gacaaatgcg caaccgatgg caatgacacc gccaataacg
    15841 ctggcccaga cggtgaaatc aattcccgtt gacgggatgg tttgcatgaa ggtgaaaata
    15901 cttggcaaac caaaggagta cactttcgtt tgcgcgtagc caatgatggt ggcccccaaa
    15961 gccccactga tacaggcgat aacaaagggg tacttacgcg gcagattaac gccatatacc
    16021 gctggttcgg tgataccaaa caggcccgtc aacgccgctg atcccgccac cacttttttc
    16081 tgcgcatcgc gttcgcagag gaagacgccg agcgccgccc cgacctgcgc cataatggcg
    16141 ggcattaaca gcgggatcat ggtgtcgtag cccagcacgg tgaagttatt gatacacagc
    16201 ggcaccaggc cccagtgcag tccgaacatg acgaagattt gccagaagcc gcccattacc
    16261 gcgcctgcaa atgcaggaac cgcctgataa agccagagat aaccggcggc aatcagttcg
    16321 cttatccagg ttgatagcgg ccccaccagc agaaaggtga cgggtgtgat aaccatcaga
    16381 catagcaatg gtgtgaagaa atttttgatt gccgacggta accacgcatt aagtcggcgt
    16441 tccagaatgc tgcacaacca ggcagaaaaa ataatgggaa taaccgatga cgagtaattc
    16501 aacaatgtga ccggaatacc caggaaatcc agccccagct catccgcttt ttgcccgttc
    16561 tcgaaagcag tcagaattaa tggatgcact aacgctccac caatcaccat ggcagtaaat
    16621 ggattaccgc cgaagcgttt ccccgcggtg tatcccagga ttatcgggaa gaaccaaaac
    16681 aaggcatcac tggcgctgaa taaaattaaa taagtaccac tttgttcggt cgtccactga
    16741 aaagtgagcg ccagagccag catacctttc aggatcccgg ttgccgccat caaaccgatc
    16801 agaggcgtaa aaatacctga aataacataa acaaagcggt ttagcagatt acctttctca
    16861 tcattttccg gtgcctgttg cgctttttcg ccaaggcctg ccacgctgtt gaccgccagg
    16921 aagacatcgg ccacatggtt acctatgacc acctgaaact ggccaccgct ttccaccacc
    16981 ataataatac cgggggtgtt tttcagtacc tcagcttgcg ctttgctttc atcctttaac
    17041 ttaaaacgta atcgcgttgc gcaatgcatc agactaacaa tgttatctgc gcccccgact
    17101 cctgcgacta tttttctggc taactccgtc ataacttgcc ctctaccgct ttgcggcaaa
    17161 actccaaaaa aaacctgaaa aaaacggcct gacgtgaatc aagcaatttt tttcaggttt
    17221 tgcccgctta gtgcggtaac aatcctttac tcagtaataa tatttcagtg ttctttgcgc
    17281 acgcgctcta tatttatcgc taaaaacata atctctgcgg gtgagatttt acgttgatac
    17341 tgcaaaccaa taaaaatggc gatccgttcc gcacattgcc atgcttgcgg gtaattttgt
    17401 tttactgctt gttgtaatga ttcatcacta tcgttaattg aagcatgttc aagaatacgc
    17461 catgataaaa acttcagatg cgtaaccagt cgctgataac tcaagctttc ttcctggtaa
    17521 ttaaggctga actgaaattt tattaattgc agcatttcgc gcattaattg cgtgacacct
    17581 gcaacatcct ccatattccc actcatttgg gcactgacca gatgcatggc aataaaaccc
    17641 acttcatctt tcggtaactg cacgcccagc cgtttatcaa taatggttaa cgcttcttcc
    17701 cctagctgga actctttcgg gtaaagccgc tggatgtccc atagcagcgg gttaggcagc
    17761 aacacgtttt gctgaaagcg tttaatcgca aactggcaat ggtcagttag cgagatataa
    17821 atactgtcct gcaactttcc caggcgctcc tgcgctaaag agataatacg atcacaggtt
    17881 gccatcacct caagaggaat atgacttaag agttcgctta atcgcccgtt cagttcatga
    17941 ctgctcaagg catactcttt ttctattcca cttgagttaa ttctttcgcc agggcgtttt
    18001 tgaaagccaa ttccgcgccc catgacgact ttttcccgct gttgatcatc aataaccacc
    18061 acaacattat tgttgagaat tttggtgatt tgcatgttca tagcaaggac cttttttata
    18121 aacaaaaaaa cccgacttca ccagtattct ctggttatgt caggttttgc ctgcgaatgc
    18181 agtaacaatc cagtcattta ttaatgattt ttataacgaa catccaggtt cgaaaattaa
    18241 tttaattgcg tgcagagaaa atagcaatgc gctattgata aaaatatgac catgctcgca
    18301 gttattaact ttgtgtaatt ttaggaattt ataaagttat atataacaaa tcccaataat
    18361 taagttattg ggatttgttt ggtgaattat ttatcgctat ctttttctgc cagcagttta
    18421 tcgagttcgt caccaccgac gtgacggaaa tcctgcccct tcacgtagta gaagataaac
    18481 tcacaaatat tctggcagcg gtcgccaata cgttcgatag agcgcgcaca gaacagcgcg
    18541 gtaagtacgc tcggaatggt gcgcgaatct tccatcatgt aggtcatcag ttgacgaaca
    18601 ataccttcgt attcctgatc gactttttta tcttcacgat aaatacgtac cgcttcgtca
    18661 atgtccatcc gcgcgaacgc gtccagcacg tcgtgcagca tctggatggt atgacggccc
    18721 agcgactcca gacttaccag caacggctga tgctgctggg agaatttctc cagcgcagta
    18781 cggcagattt tgtccgccac gtcgccaata cgctccagtt cggcaatggt tttactgatc
    18841 accataacca gacgcaggtc gctcgctgtc ggctgacgtt tggcgataat gcgcacgcag
    18901 gcttcatcga tcgccacttc catcatgttg acgttcttgt cgccttcgat gacgcgcttc
    18961 gccagatcgc tgtcctggtt atgcatcgcg gtgattgcat cagaaagctg ctgctccacc
    19021 atgccgccca tggtcatcac ctgcgtgcgg atactttcca gttcggcgtt gaactggccg
    19081 gaaatatgtt tattaagatt gagactgtcc ataacgcact cctgaatcaa ccgtaacgac
    19141 cggtgatgta gtcttctgtt tgtttcttcg ctggcttggt gaacagatcg tccgtgttgc
    19201 tgaactcaat caattcgccc aggtacataa acgccgtgtg gtcggaacaa cgcgcagcct
    19261 gctgcatgtt gtgggtgacg atcaccacgg tgtaatcctg cttcagttcg gtgatcagct
    19321 cttcaatacg cccggtagag atggggtcga gcgccgaaca cggttcgtcg agcagcagca
    19381 cttccgggcg aatggcgata ccacgcgcaa tacacagacg ctgttgctga ccaccagaga
    19441 gagagtaacc gctctggtgc aatttatctt tggtttcgtt ccacaatgcg gctttggtca
    19501 atgcccactg cacgcgctcg tccatgtcgg cacgggagag cttctcaaac agacgaacgc
    19561 caaaggcgat gttgtcgtag atggacatcg ggaacggcgt cggtttctgg aacaccatgc
    19621 ccactttcgc acgcagcagg gcgatatcct gagagttggt caggatgttg tcgccatcaa
    19681 gcagaatttc accttccgca cgctgctccg ggtacagttc aaacattttg ttgaaggtac
    19741 gcagcagcgt cgatttaccg cagccggacg gcccgataaa cgccgtaacc tggtttttag
    19801 cgatatccag gttgatgttt ttcagggcat ggaatttgcc gtagtagaag ttcaaattac
    19861 gaacctgaat tttactcggg gcagtttcaa ccatactcat tgcaatcttt tcctcatcgt
    19921 gcctgatgca cttcgcgaat caggcttacc gtaggccgga taaggcgttc acgccgcatc
    19981 cggcaaagtg ttaaccgtgt ttattcttcg caaaaacaac gcgcgccaga atgttcagca
    20041 gcagtacgca cagggtaata atcaacaccc cggcccaggc cagttgctgc cattctgcaa
    20101 acgggctcat cgcaaactta aagatcgtca ccggcaggtt ggcgatcggc tgcatcatgt
    20161 ccgtgctcca gaactggttg gagagcgcgg taaacagcag cggcgcggtt tcaccggcaa
    20221 tacgggcaat cgccagcagg ataccggtca tgatcccgga caccgacgct ttcagcgtaa
    20281 tcgcggagat cattttccac ttcggtgtac ccagcgcata agccgcttca cgcaggctgt
    20341 ccggcaccag tttcagcatg ttctcggtgg tgcggataac aatcggcacc tgcaacagcg
    20401 ccagcgcaat tacacccgcc cagccagaga agtgctccat ctgcgccacc acgatggtgt
    20461 aaacaaacag accaaccaca atcgacggcg cggagagcag aatgtcgtta atgaagcgaa
    20521 tcacttctgc cagccaggat ttacgaccat attccgccag ataaatcccc gccataatgc
    20581 ccagcggcgt accgaatacc gtggcccaca aaattaacag cccgctaccc gccagggcgt
    20641 tcgccagacc accaccttcc gtattgggcg gcggcgtcat ttcagtgaac agcgccagcg
    20701 acataccgtc gataccgcgg gtgatggtgg acattaaaat ccagatcagc cagaacaggc
    20761 cgaaggccat cgtcgccatc gagagcgtca gcgcaatacg gtttttgagg cggcgacgcg
    20821 cctgcatttt gcggcgagat tcagccagcg ccgcagtggt ttgcatttca accatagcca
    20881 ttagcgtgcc ccctcattct tagccaggcg cataatcata aacttcgatg cggcgaggac
    20941 gatgaaggta atcacaaaca ggatcaggcc cagttccatc agtgcggcaa cgtgcagacc
    21001 ggattccgct tccgcaaatt cgttcgccag cgcagaggtg atactgttgc ccggcatata
    21061 cagcgaggcg ctgtcgagct ggtaggtgtt accgatgata aaggtcaccg ccatggtttc
    21121 accgagcgcg cggcccagcc ccagcatgat gccgccgata acaccatttt tggtgaacgg
    21181 aagaacgata cgccagataa cttcccaggt ggtgcagcca ataccgtagg ccgactcttt
    21241 catcatcacc ggggtttgtt cgaacacatc acgcattacc gccgcaatgt acggaataat
    21301 catgatggcg aggatcacgc ctgccgcgag gataccgata ccaaatgcgg ggccagagaa
    21361 cagcgcgcca acaatcggga tattcgacat gatattgccg accggctcct gaaagtaaac
    21421 ggcgaacagc ggcgcaaaga taaacaggcc ccacatgccg taaacgatac ttggaatggc
    21481 tgccagcagc tcaatggcga tacccagcgg gcgtttcagc cagccaggcg caagctcagt
    21541 caggaacagg gcgataccga aactcaccgg gacggcgatc agcagcgcga taaacgaagt
    21601 caccaacgta ccgtagatcg gcaccagcgc cccgtagata tcgttcggtg catcccactc
    21661 tttggtccat aggaaagcca gaccaaattt ctgaatgctc ggccaggagg agatgatcag
    21721 agagacaata atgccaccca acatcaatag cacaatcagc gccgccagtt ttaccagcac
    21781 gctgaaaatt atgtcgccct ttttacccgg tgggttaaaa gcaggcttgg ttgcagccat
    21841 aagttactct tcagttaaac gcgtttacaa agtcattttg taatgccgga tgcggcgtaa
    21901 aacaccgtac ccggcctgga gttttattag tacagcggct taccgctact gtctttaata
    21961 ttggtcttcc acgcagcgcg aacctgttca actacactat ccggcaggct ggcgtaatcc
    22021 aggtcgttcg cctgtttagc cccggttttg tacgcccagt cgaagaattt cagcacttct
    22081 gtgccttgtt ctggtttctt ctgatctttg tggatcagaa tgaacgtggt agaggtaata
    22141 ggccatgcat cttcgccttt ctggttggtc agatcctgag cgaaggtttt gctccagtct
    22201 gcaccttttg ctgcattagc gaagttttct tcggtcggac taaccggttt accatcagcg
    22261 gagatcagtt tggtgtacgc caggttgttc tgcttcgcgt aagcatattc aacataacca
    22321 attgcacccg gcagacgctg aacgaacgcg gcgataccgt cgttaccttt accgcccaga
    22381 ccgatcggcc attttacggt agagccagta ccaacgttgt ttttccactc ttcgttcact
    22441 ttcgccaggt agctggtgaa gacgaaggaa gtcccggagc catctgcgcg gcgtactaca
    22501 gcaatgtttt gtgaaggcag tttcagaccc ggattcagtt tggcgatggc ttcatcatcc
    22561 cacttcttga ttttgcccag gtagatgtcg ccgagggttt taccatccag caccagttcg
    22621 ccagacttca gccctggaat gttaaccgcc agcaccacgc cgccaatcac ggtcgggaac
    22681 tggaacagac cttcctgagc cagtttttcg tcagacagcg gcgcgtcaga ggcaccaaaa
    22741 tcaacggtat tagcgataat ctgttttacg ccaccggaag aaccgatacc ctggtagtta
    22801 actttattac cggtttcttt ctggtaagtg tcagcccatt tggcatacac cggcgcaggg
    22861 aaggttgcac ctgcacctgt caggcttgct tctgcaaaca cagagaaagc gctcatcgat
    22921 aaggtcgcgg cgacaacagt tgcgacggtg gtacgcataa ctttcataat gtctcctggg
    22981 aggattcata aagcattgtt tgttggctac gagaagcaaa ataggacaaa caggtgacag
    23041 ttatatgtaa ggaatatgac atttttatga cagaaaggaa ttaccaaata acttactgat
    23101 tttaaatgtg aagatgaaaa tcccacatgt catctttttg tcatgatgca tttaaaagtg
    23161 ataaagaaat gggtgagatg acaacgatgt gatgaacaat cgttgtcaga cctattagcg
    23221 cacaatctca acagttatcg tcgctgttgc agtaaattgc cccggggctg ggcgagctcc
    23281 agtaaggctc gcaggagcag ctgaaaaagt tacactacct gatttttcac catacactgt
    23341 tgatttaccc atgtctgttg gcaactcacc accgttcact ttgatttcct tattattttt
    23401 gtcgtaaatt tttatcccca cgttagagtt tgaagttacg accattgaat tgtccgtatt
    23461 gggttcggta gcataaaaag acgtactgat atcatcggta ttcagtatcc ccaggttttc
    23521 acactcaata gtgaccgttt tagtaatttt atgtgttgtt accgcacttc caacaacaga
    23581 tgaaaaatct gcagcaggta tttctttaaa atcaaaataa actggctgta aattatttat
    23641 ttcgcaactt tgcggagctg aaatatcgcc ccgaatataa acatcagcca tcggctgtga
    23701 gtaatccacc ggggtgtcac ggctaattgt tccatacaat gtcactatct ttgtttcagg
    23761 aataatcaat tttcctgcaa atgtcttctt gatataaaaa tatactatcg ctttagcccc
    23821 actagatata cttaaaggtt caatgcggtt gcaattgtac tttgtattac cagacgggtt
    23881 gttagcatga tattcaaaag gtgcattc
//
LOCUS       DAPHDW010000063        23884 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.063, whole genome shotgun sequence.
ACCESSION   DAPHDW010000063 DAPHDW010000000
VERSION     DAPHDW010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23884
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.063"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(190..1638)
                     /gene="cusS"
                     /locus_tag="RH057_002467"
     CDS             complement(190..1638)
                     /gene="cusS"
                     /locus_tag="RH057_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HDV3128822.1"
                     /translation="MVSKPFQRPFSLATRLTFFISLATIAAFFAFAWIMIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFTRDAIPDKDARGGEVYLLSGPTMMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKCQVAGDPLMLRRALSNLLSNALRYTP
                     PSEAIVVRCQTVNHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVIMLPERE"
     gene            complement(1628..2311)
                     /gene="cusR"
                     /locus_tag="RH057_002468"
     CDS             complement(1628..2311)
                     /gene="cusR"
                     /locus_tag="RH057_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HDV3128823.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKRLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            2468..3847
                     /gene="cusC"
                     /locus_tag="RH057_002469"
     CDS             2468..3847
                     /gene="cusC"
                     /locus_tag="RH057_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HDV3128824.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPAMPVPQQFSLSQNGLVN
                     AADNYQNAGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDAGRYPQL
                     NGEGSGSWSGNLKGNTATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSDSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLNDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEISL
                     YTALGGGWQ"
     gene            3874..4206
                     /gene="cusF"
                     /locus_tag="RH057_002470"
     CDS             3874..4206
                     /gene="cusF"
                     /locus_tag="RH057_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HDV3128825.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEVQPQVISATGVV
                     KGIDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            4222..5445
                     /gene="cusB"
                     /locus_tag="RH057_002471"
     CDS             4222..5445
                     /gene="cusB"
                     /locus_tag="RH057_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
                     /protein_id="HDV3128826.1"
                     /translation="MKKNALIIGSMIAGGIISAAGFTWFAKAEPPAEKTSTAERKILF
                     WYDPMYPNTRFDKPGKSPFMDMDLVPKYADEESSASGVRIDPTQTQNLGVKTATVTRG
                     PLTFAQSFPANVSYNEYQYAIVQARAAGFIDKVYPLTVGDKVQKGTPLLDLTIPDWVE
                     AQSEYLLLRETGGTATQTEGILERLRLAGMPEADIRRLIATQKIQTRFTLKAPIDGVI
                     TAFDLRAGMNIAKDNVVAKIQGMDPVWVTAAIPESIAWLVKDASQFTLTVPARPDKTL
                     TIRKWTLLPGVDAATRTLQLRLEVDNADEALKPGMNAWLQLNTASEPMLLIPSQALID
                     TGSEQRVITVDADGRFVPKRVAVFQASQGVTALRSGLAEGEKVVSSGLFLIDSEANIS
                     GALERMRSESATHAH"
     gene            5457..8600
                     /gene="cusA"
                     /locus_tag="RH057_002472"
     CDS             5457..8600
                     /gene="cusA"
                     /locus_tag="RH057_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HDV3128827.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPEMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCALFLWHVRSALVAIISLPLGLCIAFIVMHFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKRLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQEQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADIKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRK"
     gene            8702..10078
                     /gene="pheP"
                     /locus_tag="RH057_002473"
     CDS             8702..10078
                     /gene="pheP"
                     /locus_tag="RH057_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HDV3128828.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGHGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(10146..11393)
                     /gene="ybdG"
                     /locus_tag="RH057_002474"
     CDS             complement(10146..11393)
                     /gene="ybdG"
                     /locus_tag="RH057_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HDV3128829.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     IDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESILNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(11501..12154)
                     /gene="nfsB"
                     /locus_tag="RH057_002475"
     CDS             complement(11501..12154)
                     /gene="nfsB"
                     /locus_tag="RH057_002475"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HDV3128830.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNERKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFADMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNITLTEV"
     gene            complement(12248..12616)
                     /locus_tag="RH057_002476"
     CDS             complement(12248..12616)
                     /locus_tag="RH057_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV3128831.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYPGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            complement(12681..12929)
                     /locus_tag="RH057_002477"
     CDS             complement(12681..12929)
                     /locus_tag="RH057_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HDV3128832.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            complement(12995..14113)
                     /locus_tag="RH057_002478"
     CDS             complement(12995..14113)
                     /locus_tag="RH057_002478"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV3128833.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNE
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTHPF
                     QKWQRQEVCENERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFASSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKMGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            14555..14707
                     /gene="hokE"
                     /locus_tag="RH057_002479"
     CDS             14555..14707
                     /gene="hokE"
                     /locus_tag="RH057_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV3128834.1"
                     /translation="MLTKYALAAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKVVL
                     AYEPKK"
     gene            15058..15210
                     /gene="hokE"
                     /locus_tag="RH057_002480"
     CDS             15058..15210
                     /gene="hokE"
                     /locus_tag="RH057_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HDV3128835.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(15332..15952)
                     /gene="entD"
                     /locus_tag="RH057_002481"
     CDS             complement(15332..15952)
                     /gene="entD"
                     /locus_tag="RH057_002481"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HDV3128836.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKAEHLAGRIAAIYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(16127..18367)
                     /gene="fepA"
                     /locus_tag="RH057_002482"
     CDS             complement(16127..18367)
                     /gene="fepA"
                     /locus_tag="RH057_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HDV3128837.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFHLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGANTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKMGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKAETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SVNTHF"
     gene            18610..19812
                     /gene="fes"
                     /locus_tag="RH057_002483"
     CDS             18610..19812
                     /gene="fes"
                     /locus_tag="RH057_002483"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HDV3128838.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWIYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGRGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDATAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKAIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            19815..20033
                     /gene="ybdZ"
                     /locus_tag="RH057_002484"
     CDS             19815..20033
                     /gene="ybdZ"
                     /locus_tag="RH057_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HDV3128839.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQASCQQWLEVHWRTLTPANFTQLQEAQ"
     gene            20030..>23884
                     /gene="entF"
                     /locus_tag="RH057_002485"
     CDS             20030..>23884
                     /gene="entF"
                     /locus_tag="RH057_002485"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin non-ribosomal peptide synthetase
                     EntF"
                     /protein_id="HDV3128840.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGDVDAPLL
                     ARAVVVGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIHVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCTW
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWAEQRRQLPPPASLSPAPLAGR
                     SASADILRLKLEFTDGEFRQLATQLSGVQRTDLALALAALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTASGPVLNVLPLGIHIAAQETLPELATRLAAQLKKMRRHQRYDAEQIVRD
                     SGRAAGEEPLFGPVLNIKVFDYQLDIPGVQTQTHTLATGPVNDLELALFPDEHGDLSI
                     EILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQINATQV
                     EIPETTLSALVAEQAAKTPDAPALADARYLFSYREMREQVVALANLLRERGVKPGDSV
                     AVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQLPRF
                     SDVPNLTSLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAIVNRL
                     LWMQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAMQQFF
                     AEYGVTTTHFVPSMLAAFVASLTPQTARQNCATLKQVFCSGEALPADLCREWQQLTGA
                     PLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPVPPGV
                     AGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEYLGRS
                     DDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINLAAATGGDARQLVGYLVSQSG
                     LPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKTQASGRAP
                     KAGSEKIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTPGQVM
                     VASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVLSRYL
                     DPQWSIIGIQSPRPHGPMQTATNLDEVCEAHLATLLEQQPHGPYYLLGYSLGGTLAQG
                     IAARLRARGEQVAFLGLLDTWPPETQNWQEKEANGLDPEVLAEINREREAFLAAQQGS
                     TSTELFTTIEGNYADAVRLLTTAHSVPFDGKATLFVAERTLQEGMSPERAWSPWIAEL
                     DIYRQDCAHVDIISPGAFVKIGP"
BASE COUNT         5557 a         6075 c         6505 g         5747 t
ORIGIN      
        1 tttagaaagg gcattgtgat ctacaattgg aagaaacaca gatttaacta agtttcatat
       61 acaaacatct cgtgaaactt tgaaaaataa aagccttcga tatttcatat gtttaataag
      121 tttttctcta atttgttgat caacaatacc aggaggtgat gcaccacgcc ctgaatttga
      181 acttaataat cactctctct ccggcagcat aatcacaaac cttgtccccc gcgcatctga
      241 cgttaccgca accgtgcctt tatgcgcgac aacaatcgat ttcactatcg ccagcccaat
      301 accgctacct tcaccttttc gctggcggga agggtcaacg cgatagaaac ggtcaaacaa
      361 tcgcggtaag tgctcgggcg caatgggcgt accggggttt tcgacggaaa cttgcacctg
      421 atgattgacc gtctggcagc gcactacaat agcctcactg ggtggcgtat aacgcagggc
      481 attagaaagc aggttgctta acgcccgacg cagcatcagc ggatcgcccg caacctgaca
      541 tttgtcgcca acaaaccgca actccacgcc gcgatcttcc gctaacgcct cgaaaaaatc
      601 gaacactttg ccgacttcat ccgccaggtt gagcattttc ttttcgggga ttagctggtt
      661 gttatcggcc tgagcgagaa acagcatatc gctgaccatt ttcgccattc gcgtcagctc
      721 ttcgagatta gagtagagca catcttccag ttccttctgg ctgcgcgact ggctgagggc
      781 gatttccgtt tgcgttatga gattagtaat tggtgtgcga atttcgtgag cgatatccgc
      841 tgagaaattg gactggcggg taaagacatc ctcaatacgc tcgatcatat ggttgaacga
      901 cagtaccagc tgttccagct caatgggcac ggtctgcggg tcgaggcgaa cgtcgagatc
      961 tttcgaggta atattctgga tttgacggct gacgctgcgg atcggcgcgt gacctttatg
     1021 taccgccaat agcacgataa agacgatcag gatgctgatg acagatgcgg tcataataag
     1081 tttattcatc aaatcattta tgtaatgaag atgaaaatcg atcgaaagcg cgatgtagag
     1141 cgtataaatc ggtttgccgt ccaccaacgg gccaaccggc aagttaatca tccgccagtt
     1201 gctgtgttcc atatgcccgt gaccgtggcc tggcatcatc atcgtcgggc cggaaaggag
     1261 atacacctca ccgccccgcg cgtctttatc gggtatggcg tcacgcgtaa actcgcggat
     1321 atccggcgca ccgggggagt gatacaccgt tttaccatgg ctatctgcca gggagatcaa
     1381 cacgttggaa taaccactga cgatatcttc cagcgtcatt aagcgtcggg cttgcgtctc
     1441 gtcaggatga ttaagtaccc gttcaagcgt ggcgctaatt tcttttaaat cattaatatc
     1501 ctgctcggca aaatgcactt ttactgagtg gatcatgatc catgcaaagg cgaaaaacgc
     1561 tgcgatggtg gccaggctga taaaaaaggt caggcgggtt gccagcgaaa acgggcgctg
     1621 aaatggctta ctgaccatcc ggcacctcaa gcatgtaacc cacgccgcgc acggtctgaa
     1681 ttagctttgg ctcaaagtcg ttgtcgattt tgccgcgcag ccgcttcacc gctacatcaa
     1741 tggcgttagt gtcgctgtcg aaattcatgt cccacacctg cgaggcgata agcgagcggg
     1801 gcagcacttc gccctgatgg cggaggaaaa actccagcag agtaaactct ttactggtca
     1861 gagtgatgcg cgtgccgctg cgggtgactt tgcggctgac gagatcgacc atcagatcgg
     1921 caacctgaaa ctgactttcg ataatcaccg ccgccccgcg ccgcaggaga gtgcgcaccc
     1981 gcgccagcag ttcagcaaaa gcgaacggct tcaccagata gtcatccgcc cccaactcca
     2041 gccccttgac gcgatgttca atggtgccaa gcgcggtaag caacagaatc ggcatccctt
     2101 tattggcgga acgcaacatg cgcacgatat cccagccgtt cacgtccggc agcataatat
     2161 cgaggattat cagatcataa tcaccggtca tcgccagatg gtagccattc aacccgttgt
     2221 cggccaaatc gaccacaaaa ccggcttcgg ttaacccttt ggtcaagtat tctccggttt
     2281 tcttttcatc ttcgacaatc aacagtttca tatttcctcc gcatgttgcc cgggcaattc
     2341 tagagtagcg ggatcagatg gcaatcgctt attggcaaaa tgacaatttt gtcatttttc
     2401 tgtcaccgga aaatcagagc ctggcgagta aagttggcgg cataaaatca ccagaaatta
     2461 tgagcctatg tctccttgta aacttctgcc attttgtgtg gcccttgcgc taaccggttg
     2521 ttcactggca ccggattatc agcgtccggc aatgcccgtg ccgcagcagt tctcactcag
     2581 ccagaacggc ctggttaacg cagcagataa ctatcagaac gcgggctggc gcaccttttt
     2641 tgttgataat caggtgaaga cgctgattag cgaggcgctg gtgaataacc gggatttgcg
     2701 catggcgacg ctgaaagtgc aggaggcacg ggcgcaatat cgtctgaccg atgccggccg
     2761 ctacccacag ctcaatggcg agggcagcgg cagctggagc ggtaatctta aagggaatac
     2821 cgccacgaca cgggagtttt cgaccggcct taacgccagc tttgacctcg attttttcgg
     2881 tcgcttaaag aacatgagcg aagccgagcg acaaaattat ttagccactg aggaagctca
     2941 gcgcgcggtg catattctgc tggtttctaa tgtcgcgcaa agctatttca atcagcaact
     3001 ggcgtatgcg caattgcaaa tagccgaaga aacgctgcgt aattatcagc agtcatatgc
     3061 gtttgtcgaa aaacaactgt tgaccggtag cagcaatgtt ctggcgctgg aacaggctcg
     3121 cggggtgata gaaagtaccc gcagtgacat cgctaaacgt cagggggaac tggcgcaggc
     3181 gaataatgca ttgcaactgt tattgggaag ctacggcaag ctgccgcaag cgcagacagt
     3241 aaacagcgac agcctgcaaa gcgttaaatt accggcgggc ttgtcgtcgc aaatcttatt
     3301 gcagcgcccg gatattatgg aagctgaaca cgcgttaatg gcggctaatg ctaatattgg
     3361 tgctgcgcgt gcggcatttt tcccctctat cagcctgacc agtggaatat caaccgccag
     3421 tagcgatcta tcctcattat ttaacgccag cagcgggatg tggaatttta tacccaaaat
     3481 tgagatcccc atttttaatg ccggacgcaa ccaggccaat ctggatatcg ccgaaattcg
     3541 ccagcagcag tcggtggtga attatgaaca gaaaatccag aacgccttta aagaagtggc
     3601 agatgcgctg gcattacgtc aaagcctgaa cgatcaaatt agcgcccagc agcgttatct
     3661 ggcgtcgctg caaattaccc tgcaacgggc acgggcatta tatcagcacg gcgcggtaag
     3721 ttatctggaa gtgctggatg ccgagcgctc tttatttgca acccgacaaa ctttacttga
     3781 cctgaattat gcccgtcagg ttaacgaaat ttctttatat accgcacttg gtggcggttg
     3841 gcagtagtaa tttttaactc caggagagaa taaatgaaaa aagcactgca agtcgcaatg
     3901 ttcagtctgt ttaccgttat tggctttaat gcccaggcta acgaacatca tcatgaaacc
     3961 atgagcgaag tacaaccgca ggttattagc gccactggcg tggtaaaggg tattgatctg
     4021 gaaagcaaaa aaatcaccat ccatcacgat ccgattgctg ccgtgaactg gccggagatg
     4081 accatgcgct ttaccatcac tccgcagacg aaaatgagtg aaattaaaac tggcgacaaa
     4141 gtggcgttta attttgtcca gcagggcaac ctttctttat tacaggatat taaagtcagc
     4201 cagtaaccca ggtttaatga gatgaaaaaa aacgcgctta ttatcggcag catgatcgcg
     4261 ggcggtatta tttctgcggc aggttttacc tggtttgcaa aggctgaacc acccgcagaa
     4321 aaaacgtcga ccgcagaacg taaaatctta ttctggtacg acccgatgta ccccaatacg
     4381 cggttcgata aaccaggtaa atcgccgttt atggatatgg atctggtgcc gaaatatgcc
     4441 gatgaagaga gttctgcgtc tggtgtgcgc attgacccga ctcagacgca gaatctgggg
     4501 gtgaaaacgg ctaccgtcac gcgcgggccg ctgacttttg cccagagttt cccggcgaat
     4561 gtcagttaca acgagtatca gtatgccatt gtgcaggctc gcgctgccgg gtttatcgac
     4621 aaggtgtatc cgcttaccgt gggcgataaa gtacaaaagg gcacaccgct tctcgacctg
     4681 accattcccg actgggtgga agcgcagagt gagtatttac tgctgcgcga aacgggcggt
     4741 acggcgaccc agactgaagg cattcttgag cggctgcgac tggcgggaat gccggaggcg
     4801 gatattcgcc gcctgatcgc cacgcaaaaa atccagactc gctttacgct caaagcaccc
     4861 attgatggtg tgatcaccgc gtttgatctg cgcgcgggaa tgaatatcgc caaagataac
     4921 gtggtagcga aaattcaggg tatggacccg gtgtgggtca ctgctgcgat cccggagtct
     4981 atcgcctggc tggtgaaaga tgcctcgcag tttacgctca ccgttccggc acgaccggat
     5041 aaaacactca ccatccgcaa atggacgctg ctacctggcg tggatgccgc gacccgcacg
     5101 ctgcagctgc gtctggaagt cgacaacgcc gacgaggcgc taaaaccggg catgaacgcc
     5161 tggttgcaac tcaacaccgc cagcgaaccg atgctgctca ttccgtcaca agcgctgatt
     5221 gataccggca gcgaacagcg ggtgattacc gttgatgccg acgggcgctt tgtaccgaaa
     5281 cgcgttgctg ttttccaggc atcgcaaggc gtcaccgcat tacgctctgg tctggcggaa
     5341 ggtgaaaagg tggtttccag cggcctgttc ctgattgatt ccgaagccaa tatttctggc
     5401 gcactggagc ggatgcgctc tgaaagtgct acccatgcgc attgagggaa taaccaatga
     5461 ttgaatggat tattcgtcgc tcggtggcga accgtttttt ggtgctgatg ggcgcgttgt
     5521 ttctgagcat ctggggcacc tggaccatca ttaacacgcc agtggatgcg ctgccggatc
     5581 tctccgatgt gcaggtgatt attaaaacca gctatcccgg tcaggcaccg caaatcgttg
     5641 aaaatcaggt gacttatccg ctaaccacca ccatgttgtc ggtgcctggc gcgaagactg
     5701 tgcgcggttt ctcgcagttt ggagactctt atgtgtatgt cattttcgaa gatggtaccg
     5761 atccgtactg ggcgcgttcg cgggtgctgg agtacctcaa ccaggtacag ggtaagctgc
     5821 ctgcgggagt cagcgccgag ctgggaccag atgccacggg tgttggctgg atctatgaat
     5881 atgcactggt ggatcgcagc ggtaagcacg atctggccga tttacgctca ttgcaggact
     5941 ggtttctcaa atatgagcta aaaaccatcc ctgacgttgc ggaagtggcg tcggtgggcg
     6001 gcgtggtgaa agagtatcag gtggttatcg atccccagcg cctggcgcaa tatggcatca
     6061 gtctagccga agtaaaaagc gcgctggatg cttcaaacca ggaagcgggc ggttcgtcga
     6121 tcgaactggc ggaagcggaa tatatggtgc gcgccagcgg ctatctgcaa acgctcgacg
     6181 actttaatca catcgtttta aaagccagtg aaaatggcgt gcccgtttat ctgcgcgatg
     6241 ttgcgaaggt ccagattggc ccagagatgc gccggggcat tgccgaacta aacggcgaag
     6301 gcgaagtggc gggcggggtg gtgatcctgc gatccggcaa aaacgcccga gaagtgatcg
     6361 ccgccgtgaa ggacaaactg gaaacgctga aaagtagtct gccggaaggc gtggagatag
     6421 ttacaacata cgatcgcagc cagctcattg accgcgccat cgacaacctc agcggcaagt
     6481 tgctggaaga gtttattgtg gtggcggtag tctgtgcgct gtttctctgg catgtgcgct
     6541 cggcgctggt ggcgattatt tcgttgccgc tggggttgtg tattgctttt attgtcatgc
     6601 acttccaggg actgaatgcc aatattatgt cgttgggcgg cattgcgatt gccgtcgggg
     6661 cgatggtcga tgccgctatc gtcatgatcg aaaatgcaca taaacggctg gaagagtggc
     6721 agcaccagca tcctgacgcc acgctggata ataaaacgcg ctggcaggtg atcaccgatg
     6781 catctgttga agtgggtccg gcgctattta tcagtctgct gattatcacg ttgtcgttta
     6841 tcccgatctt caccctggaa gggcaggaag ggcgtctgtt tggcccgttg gcgttcacta
     6901 aaacctatgc gatggcgggc gcggcgctgc tggcgatcgt agtgatccct atcctgatgg
     6961 ggtactggat ccgtggcaaa attccgccgg aaagcagtaa cccgctcaat cgctttttga
     7021 ttcgtgttta tcatccgctg ttgctgaaag tactgcactg gccgaaaacc acgctgctgg
     7081 tggcagcgct ttcggtgctg acggttctct ggccgctcaa taaagttggc ggggaatttt
     7141 taccgcagat caatgaaggc gacttgttgt atatgccatc gacgctgccg gggatttccg
     7201 cagcagaggc ggcgagtatg ctgcaaaaaa ccgacaagct aattatgagc gtacctgaag
     7261 tggcgcgggt atttggcaaa accgggaaag cggaaaccgc caccgattct gctccgctgg
     7321 agatggtaga aacgaccatc cagcttaagc cgcaggagca gtggcggcca ggcatgacga
     7381 tggacaaaat cattgaggaa ctggataaca ccgtgcgtct gccggggctg gcgaatctgt
     7441 gggtgccgcc aattcgtaac cgtatcgata tgctctcaac cggcattaaa agccccatcg
     7501 gcattaaagt ttccggcact gtgctggcgg atatcgacgc gatggctgag caaattgaag
     7561 aagtggcgcg aacggtgcca ggcgtagctt ctgctcttgc cgagcggctg gaaggtgggc
     7621 gctatatcaa cgttgagatt aaccgtgaaa aagccgcgcg ttacggtatg acggtggcgg
     7681 atgtgcagtt gtttgtgact tctgctgtgg gcggggcgat ggttggcgaa acggtggaag
     7741 gaattgcccg ttatccaatt aatctgcgtt atccgcaaag ctggcgcgat agtccgcagg
     7801 cactgcgcca gctgccgatt ctgacgccga tgaagcagca aatcaccctg gcagacgtgg
     7861 ccgacattaa agtctctacc ggaccgtcaa tgctgaaaac cgagaatgcg cgcccgacga
     7921 gctggattta tatcgatgcc cgcgatcgtg acatggtgtc ggtggttcac gatttgcaaa
     7981 aagcgatagc tgaaaaagtg cagttaaaac cgggcaccag cgtggcattc tccgggcagt
     8041 tcgagctact ggagcgcgcc aaccataagc ttaaactcat ggtgccgatg acgttgatga
     8101 ttatcttcgt gctgttgtat ctggcgttcc gtcgggtggg cgaagcgttg ctgattatca
     8161 gcagcgtacc gtttgcgctg gtgggcggca tctggttgct gtggtggatg ggctttcatc
     8221 tttccgtggc gacgggcact ggctttatcg ccctcgccgg ggtcgccgcc gaatttggcg
     8281 tggtgatgct gatgtattta cgtcacgcca tagaggccga gccgtcgttg aataatccgc
     8341 aaacattcag tgagcagaag ctggatgagg cgttatatca cggcgcggtc ctgcgcgtgc
     8401 gcccgaaagc gatgacggtg gcggtgatta tcgccggtct gctgccgatt ctgtggggaa
     8461 cgggggctgg ttcagaggtg atgagccgga ttgccgcacc gatgattggc ggcatgatca
     8521 ccgcaccttt gctgtcgctg tttattatcc cggcggcgta taagctgatg tggctgcacc
     8581 gacatcgggt acggaaataa aagcaggata ccccgtttaa ccgtgtggat tgtgtcttgc
     8641 gacgatgggc actaaatgtt aaaaggtgcc cctcaacaaa aaagacacac aggggaaagg
     8701 cgtgaaaaac gcgtcaaccg tatcggaaga tactgcgtcg aatcaagagc cgacgcttca
     8761 tcgcggatta cataaccgtc atattcaact gattgcgctg ggtggcgcaa ttggtactgg
     8821 tctgtttctt ggcattggcc cggcgattca gatggcgggt ccggctgtat tgctgggcta
     8881 cggcgtcgcc gggatcatcg ctttcctgat tatgcgccag cttggcgaga tggtggtcga
     8941 agagccggta tccggttcat ttgcccactt tgcctataaa tactggggac cgtttgcggg
     9001 cttcctctct ggctggaact actgggtaat gttcgtgctg gtgggaatgg cagagctgac
     9061 cgctgcgggc atctatatgc agtactggtt cccggatgtt ccaacgtgga tttgggctgc
     9121 cgccttcttt attatcatca acgccgttaa cctggtgaac gtgcgcttat atggtgaaac
     9181 cgagttctgg tttgcgctga ttaaagtgct ggcgatcatc ggtatgatcg gctttggcct
     9241 gtggctgctg ttttctggtc acggcggcga gaaagccagt atcgacaacc tctggcgcta
     9301 cggtggtttc tttgccaccg gctggaatgg gctgattttg tcgctggcgg taattatgtt
     9361 ctccttcggc ggtctggagc tgattgggat tactgccgct gaagcgcgcg atccggaaaa
     9421 aagcattcca aaagcggtaa atcaggtggt gtatcgcatc ctgctgtttt acatcggttc
     9481 actggtggtt ttactggcgc tctatccgtg ggtggaagtg aaatccaaca gtagcccgtt
     9541 tgtgatgatt ttccataatc tcgacagcaa cgtggtagct tctgcgctga acttcgtcat
     9601 tctggtagca tcgctgtcag tgtataacag cggggtttac tctaacagcc gcatgctgtt
     9661 tggcctttct gtgcagggta atgcgccgaa gtttttgact cgcgtcagcc gtcgcggtgt
     9721 gccgattaac tcgctgatgc tttccggagc gatcacttcg ctggtggtat taatcaacta
     9781 tctgctgccg caaaaagcgt ttggtctgct gatggcgctg gtggtagcaa cgctgctgtt
     9841 gaactggatt atgatctgtc tggcgcatct gcgttttcgt gcagcgatgc gacgtcaggg
     9901 gcgtgaaaca cagtttaagg cgctgctcta tccgttcggc aactatcttt gcattgcctt
     9961 cctcggcatg attttgctgc tgatgtgcac gatggatgat atgcgcttgt cagcgatcct
    10021 gctgccggtg tggattgtat tcctgtttgt ggcatttaaa acgctgcgtc ggaaataagg
    10081 cattcacgct acatccgaca aaacgatgtc cgctctcatc cattcgatga gagcggtttt
    10141 tttaattact gcttaaatgc acccgccaga gagcgaatat cattgccggt tggcgactga
    10201 tgaagtcgca gaccaaactc ttcgacaatc gcaaatatgt gatcgaatat atcagcctga
    10261 atgctttcat attccagcca caccacggtg ttagtaaacg cgtagatctc gagcggtaaa
    10321 ccgttatcac ctggagccag ttggcgtacc attaaggtca tatctttacg aatccgcgga
    10381 tggttacgca gatattcgtt cagataggca cgaaaggttc caatattggt cattcggcgc
    10441 aggtttaata tcgactccgt agaaccttgc tggcgattcc actcattaat ttcctgatgg
    10501 cggctggtta aataaggttt taacaagtgc gctttattca gacgttgcat ttcgtcttca
    10561 tcaataaaac gaatactggt gacatcaata ctgatactgc gcttaatacg tcgcccgcca
    10621 gatgctgaca tcccgctcca gtttttaaag gagtcagaaa ccagagacca ggtgggaata
    10681 gtggtaatgg tattgtccca gttacgcact ttgacggtgg ttaacccaat atcgatcacc
    10741 gcgccatccg cgccgtattt cggcatctcc agccagtcgc ccagtttcag catatcgttc
    10801 gcggaaagct gaatacctgc caccagacca agaatcggat ctttaaaaac caacatcagc
    10861 acggcagcca ttgcaccaag accgctgatc agaatcgctg gcgactgacc aatcagcagc
    10921 gagatcatca aaatgccgac caggattgcg ccgatcagtt taatcccctg aaatatccct
    10981 ttcagcggta actgagatgc cgccgggaat ttctgcgcca gattcaaaat aacatccagc
    11041 aacgagaaga ctgaaagcag cgcatacatc atgatccaca actgcgcgca ggtagtcaga
    11101 atatctgccg cttcggtgcc tttttgcagc cagaataccg cctgaatatt gacgataatc
    11161 ccctgcaggg taaaagctaa acggtggaag agtttattct gggtaatgat ttgcaaccaa
    11221 agccgtgaac tggcgatggc acgtttttcg aaggtccgca gtaccaccca atgcaaaata
    11281 atatgcacca cgacggcggt cagaaaaata ataccgaaaa tcatcaccat cgaggtggtg
    11341 tgatcgatct caatacccgc taaatcttca acctgggata ttaaatcctg cataacgtct
    11401 cctttataca acagcagcct atgatgacgg ctgaaacagg gttatgcaaa tcaggagaat
    11461 ctgaggggaa atagccgggc agatgcccgg caagagagaa ttacacttcg gttaaggtta
    11521 tgttttgcgg cagacgagat ttcggcagcg tagcgttaaa atcttcaacg ctgtgatgac
    11581 ctaccggaac aaccaccaga ctggtgtagc ctttctcttt cagaccaaat tctgcatcga
    11641 ggatggcggc gtcaaaacct tcgatgggta ccgcgtccag acccagcgcc gccacgccga
    11701 gcaggaagtt accgacgttg agataaacct gttttgccat ccactctgca tcatcatgca
    11761 gatctttacg gtgcatatca gcgaagaact tgcgaccttt atcgttcgcg gctttcgctt
    11821 ccggcgtggc aaagcggcca tcagcatctt cctggtcaac aaccagcttc agccaggcat
    11881 cgtccatcgc ggtttttgca cagaacacca cgacgtgcga ggcatcaagt attttacgtt
    11941 cgttgaacac ataattaccg gcagcggatt tggcaacacg cgctttacct tcttccgtgc
    12001 tggcaacaat aaaatgccac ggctgggagt tggtgctgga tgggctgtat tgcaggagag
    12061 ttttgatctg ctcggcctgt tccggggtaa gttttttact ggcatcaaat gccttagtgg
    12121 aatgacgctt taaggcgaca gaaatgatat ccataaagac tccatgtgaa agtaattttg
    12181 cgtgccagca gattacaagg ttcaacgaga aatggtaagc gagaaaaatg cgctatagat
    12241 ttccgcttta gcctggacgc actctttttt gatcgcgttt agccagacca tcaaccacca
    12301 gattccacga atcgttgatc agatcgcgaa gtaacgcttc ggagatttcc tcgccgggat
    12361 acaccgaaat ccagtgcttt ttattcatgt gataccctgg cttaatgctc gggtatatct
    12421 gctgatttaa cagggatttt tgtggatcgg acttcagatt gataaagggg acgccgcgca
    12481 gctccgacga cagcataaaa attttgccgc caattttaaa aacatcgaac tccgggccaa
    12541 aaggccagca aagctcgaca aagggtaact caagggccag gcgtttcgcc gtttcgtgca
    12601 gtgattgctt atccataaac gttcctttag gcgaaggaga ataagcaaag tatgccgcga
    12661 agtacggcga taatcgacgt ttaatccgcc agcgagaacc agcgtcgcca gataaagcgc
    12721 agaacaaaat actcaatagc gcccagcact aaaaaccaca gacaaaacaa taaagtgtaa
    12781 agctggctaa gatccatcag atggaacatg gtcaccagtt tttgtgccag cgtcagcccc
    12841 agtgcggggg cgggcagcag caggcaagag aggaaagcca tcagcaaaat gcctgctgcg
    12901 gtagtcaagg tttctaaagg gtgtttcata acattgttaa atgtaagtta aaacaccatt
    12961 gtcagggata ttcttctgta aggcaattcc ctacttagtc accggcccag atctcacaat
    13021 gctttttcac cagcccaatc agcgagccac catcggcgac gaaatcgcgc atcagctgcg
    13081 cttcattcag accgctgacg acctggcgat gcagggcctc aatcgcgctc gatgcaccca
    13141 ttttatgtgc ggaaggggcg attttttcca gcaatcgcaa ggtatcttcc gttagcggtc
    13201 gacgatcgcc agtgtgcgga tcggtaatga cgccttccag cccatagcgg caggcctgga
    13261 aacggttgaa tttatacagc aggtaatctt tctcctgatg tttaaacggg cgttccgtca
    13321 gtaaccagtg ggcggtggcc tgaattaatc ccgccatatt taccgcgtgg ctaagggtta
    13381 acggggtatc catcacccga acctccaccg tgccaaaatg aggactgggg cgaatatccc
    13441 agtgcagatc tttaatgctg tcgatcatcg tggtgtaact caggcagcga aacagggctt
    13501 caaattgttg ccagttactg acccacggca tcgggccatt atcgggaaag gcggaaaaaa
    13561 tattcggtcg tgaggaggca aaacgcgtat ccgttccctg catatacggc gacgcggcgg
    13621 aaagggcgat gaagtgcggc acaaaccgtg acaggccgtg cagcaaataa atggcgtcat
    13681 cgccactggc gcagccaaca tggacatgct gaccaaaaac ggtcgcctgc tggatgagat
    13741 agccaaagtt ttccagcgtt cgttgatagc gttcgttttc gcatacctcc tgacgctgcc
    13801 atttctgaaa cgggtgcgtg ccaccgccgc aaatttccag atgatggtct gcggctgcct
    13861 gcaatacgac tttctgcatc gctgaaaact gcccggcagc ctggttgata tcacggcaaa
    13921 catccgtcgc cagctccagc atactttcgg tgatatcgtg ctttacctct ccggccgtga
    13981 tctcattttt aaccgcgtca atcagcattg aagagtcctg gcttaagtca tagcccggcg
    14041 gattaaccac ctgcatttcc agttcaatac cgagggtaaa aggttcagaa acatgaaaat
    14101 cgggtaatgg cataggtttc tcttaagttg gcgttttcta ttcagtatag aagtcggagc
    14161 ggctgggcga gatgcggaag ttctggaatg tttctttttt tggtgatggt gactgaagca
    14221 atttggctac ttttgcaatg tgacaagtta tggcacggct ggctggtggc gaagaatttt
    14281 gacgattgag gcatgcagaa aaaaacgggt tcagcttcag ttgatcaaat ggggagtgag
    14341 aagtccgaaa caggacttac tgtataaata aacagctatt ttgctaagca agggtaagat
    14401 aactgcgggt gcctgaagct ttccggtttc aggtttactc tgaggtctgg aaagatgaag
    14461 ccccaggacg atatttctat caaccctggg gctacctacc caaacctgaa caatttggat
    14521 ggtagtccct tcttcgcatg gaggcaatat aaacatgctg acgaaatatg cccttgcggc
    14581 agtcatagtg ctgtgtttaa cggtgctggg atttacgctt ctggtcgggg attcgctgtg
    14641 tgagttcacc gtaaaggaac gtaatattga gtttaaggtt gttctcgctt acgaaccgaa
    14701 gaagtagccg ttgtgcgggg agcaatcccc gcatatccgg ttgtcaggtc aggatggtaa
    14761 ggcacctgct ttacactttc gcccgcggtc agtgatggct gcgggcgatt tcgttttaag
    14821 agcaatgatg agttttgccg acagaagtcc gaaacaggat tcactgtata aataaacagc
    14881 tattttgttg aggaagggta agataacggc gggtgcctga agctttccgg tttcaggtta
    14941 actctgaggt ctggaaagat gaagccccag gagatatttc tatcaatcct ggggctgcca
    15001 ctccgagcct caacaactcg gatggtagtc ccttcttcgc atggaggcaa tataaacatg
    15061 ctgacgaaat atgcccttgt ggcagtcata gtgctgtgtt tgacggtact gggatttacg
    15121 cttctggtcg gagactcgct gtgtgagttc accgtaaagg aacgtaatat tgagtttaag
    15181 gctgttctcg cttacgaacc gaagaagtag ccgttgtgcg gggagcaatc cccgcatatc
    15241 cggttgtcag gtcagagtgg taaggcacct gctttacact ttcgcccgcg gtcattgatg
    15301 gctgcgggcg aatcgtacca gatgttgtca attaatcgtg ttggcacaga gttatgacta
    15361 ttttttcttt tatctgccag tgcacagcaa acatctcatt ctcacgatga atgatgacct
    15421 gctgtttatt ccagctaatt atctgatagt ccagaaaacc tgcatcagtt tggatctcac
    15481 ttgccttaaa tgcgctctct ttggcggaaa atgccagtgt cagcgccaga gaaaaggcta
    15541 aaccgcagtc tgcgagtcgc tcgtgttctg ctggtgtaat aatgttgtct gtcaattcgg
    15601 tcgctgtttg cgcagaaaaa atttcttcga tatcaacgcc aatcggttga cgggaaacca
    15661 cggccaatgc cgtagccccg cagtggctaa tactgccgta tacctccgca ggccacaccg
    15721 gttggcgtag ctcgccgatt gcgggcacac atttatagcc atattcccgc agcgcataaa
    15781 ttgcagcgat ccgcccggct aaatgttcgg ctttgcgctt ccgtccagcg tgttgcagtt
    15841 gcgcgtaatg cggcagccag agtaaatcct gctcacaaaa attcgccgga tcgaactcaa
    15901 caaaatgcag cgtatgtccg gcaaagggga gggaggtatg cgtagttttc atgtcgacca
    15961 tattagagac tgatgacaaa cgctaaactg cctgatgcgc tacgcttatc aggcctacat
    16021 ggtcctgcaa tatattgaat tggcaagatt tttgtaggcc ggataaggcg ttcacgccgc
    16081 atccggcatg aacgacgcgc actttgtcaa caatctgacg ttagcatcag aagtgagtgt
    16141 ttacgctcat gtaccacgta cgtcccggct cgttataggt atacgccccg gcacccgcga
    16201 tatagttggc ccctgccaga tcgcccgtgg tctgggcatt acccgcacgc cacaaacgtt
    16261 tgtcgaacag gttgtccacg ccgccggtca gactgacatt cttcgtcaca tcccaggtcg
    16321 cgctcagacc cacaatgctg taaggactga tttctttggt ttccgggcca accgctggct
    16381 gacctttgta gttgtatttt ttcggctgct gcttaccgta ccaggtgaag gtcgtttgca
    16441 tcgacaaatc ttcccgcgcc tgccagctca gcgttgagtt caacgtgtac tccgggatga
    16501 tcgacaaacg gtcgcccgtg gttttgtttt cactcttcag catataagtg atgttattgg
    16561 tccacatcac cgtttcgcta actggtacgt ttaacgatcc ttccagacct tcaaccactg
    16621 ctttcggcac gttatcccac tgatagagat cggtgccgac tgcgttttgc cctacagcca
    16681 catagcccgc ttcaatctta ttgcgataat cgttacggaa ccaggtgacg cccgccagcc
    16741 agccgtcgcg tttgaactcc agaccaatct ctttgttgat gctggtttct gctttcaggt
    16801 catcgttacc ttgcagatag cagccgcccg cgctggcata gcagccctga cctttactgt
    16861 agagaatgta gttcgggtta gtctggtaca ggctcggtgc tttataagca cgggcaatgc
    16921 ccattttgag cgtgaagtca tcgcctaaac cttgcgatat gttcagcgcc gggctccagt
    16981 tattgccgac aatactgtga tgatcgaaac gcagccccgg cgttacgatg gtgctgtcag
    17041 tcagctccat gttgttttcg gcaaacagcg agaaaatttc tgcttttgaa tacgggctac
    17101 ggtcggtggc actcacacca tcaatagcgc cgccggtgtt cgccccggtc agcgcctggg
    17161 tattggaact caagtccttc atccgttgct gattccactc cgtacccagc gtcagcgtct
    17221 ggttaacgag gaaatcaatc ggcaggttaa cttcgctgtg cagcatcacg tcatcgagat
    17281 cgatatcgac gaaatcctgt gtcgcttttt cgttaaattt cccttcggta ccgcccgcca
    17341 gaccttccgg aatacgcgag ttacgggtgt gttcatactg cacccagttg ctggtggtca
    17401 cgccgttatc ccagccaccg ttccaggtca gcgcgtagtt ctggcgatac aggcggttgg
    17461 tttcatcgcc atatttcgag cgagtgtaag cgtcagagtt ggtgttctgg gtatcgcccg
    17521 catacaggtt accctggcgg ctgtaacccg cttccagttc cagcgattgc aatggtgcga
    17581 agtcccagcg caccacgccg ttaatatctt tattgatcac cccttcgcgc ccggctggta
    17641 acgtcgtggc atacgttccg gcacgcgcgg actgatggcc ctggttgata tcccacgcgt
    17701 cagcctgggt tttgtcgagg ttgccataca agtggaagct gaattcgtcg cccagcggac
    17761 cggtcaggct gaagttggtg cgtttagtgg caccttcctc tttatgttct ggtgcattga
    17821 aataagcgtc ccaggagccg tgccactcac cgctgccttt tttggtaatg atgttaacca
    17881 cgccgcccgc cgcgccgttg ccataacgcg cagctgccgg accacgcaga acttcaatac
    17941 gttcaatcat ttcaggtggc acccaggagg tatcaccacg ggtatcgcgc tcgccacgcc
    18001 agccctgacg caccgagtta cggctgctta ccggcttgcc gtcaatcaaa atcagcgtgt
    18061 tttccggacc cataccgcga atatcaatct gtcggttatt cccacgctga ccactggtgg
    18121 agttaccggt caggttaacg cctggcatgg tacggatgat ctccgacaca tcgcgggcaa
    18181 cggggttttt gcggatttca tctgcggtga tggtcgaaac gccaggcgcc tgtaagttct
    18241 gctcggcggc ggtaacgaca atagtatcgt catgtgaaac aggagtatcg gtcggctctt
    18301 gtgcctgtgc taccccataa atccccagat tgaccaacaa ggccagggaa tgaatcttct
    18361 tgttcattgt tttattcctg catttttgcc acgaattgca actgtcgggc atggtcgtcc
    18421 tcaacacgac gcatcccgct accgcgaaaa cctttgatcc tgaaagacac gcagtgcagt
    18481 tggttaataa atgtccgcgc ttcccacggc gcgccattac gctattgcaa atgcaaatag
    18541 ttatcaataa tattatcaat atatttctgc aatcaatgaa aaattgcaca gtaaacatgg
    18601 ggttatggtg tgacggcgtt aaaagtagga agtgagagct ggtggcagtc gaaacatggc
    18661 ccggaatggc agcgtctgaa tgacgaaatg tttgaggtca ctttctggtg gcgtgatccc
    18721 caaggttctg aagaatactc gacgataaag cgcgtatgga tctacatcac tggtgtgacc
    18781 gatcatcatc agaacagcca gccccagtcg atgcagcgaa ttgcaggtac tgatgtctgg
    18841 cagtggacga cacaactcaa tgccaactgg cgcggcagct actgctttat tcccaccgaa
    18901 cgcgatgaca ttttttctgc accatccccc gatcgcctcg aattacgtga aggctggcga
    18961 aaactattac cccaggcaat agccgatccg ctgaatccac aaagctggaa aggcgggcga
    19021 gggcacgctg tttctgcact cgaaatgccg caagcgcctc tgcaaccggg atgggattgt
    19081 ccgcaagcgc cagaaatacc tgccaaagaa attatctgga aaagtgaacg gttgaaaaat
    19141 tcacggcgtg tatggatttt taccaccggc gatgcaacag cagaagaacg cccgctggca
    19201 gttttgctcg atggcgaatt ttgggcgcaa agtatgcccg tctggccagt gctgacttcg
    19261 ctgacccatc gtcagcaact tcctcccgcc gtgtatgtgt tgatcgacgc tatcgacacc
    19321 acgcaccgcg cccacgaact gccgtgtaat gcggatttct ggctggcagt acagcaagag
    19381 ttattacccc tggtgaaagc tattgcccct tttagcgatc gcgccgatcg caccgtggtc
    19441 gccgggcaga gttttggtgg gctgtccgcg ctgtatgccg gactgcactg gcctgaacgc
    19501 tttggctgtg tattaagcca gtcaggatcg tactggtggc cgcatcgggg cgggcagcaa
    19561 gagggcgtgt tacttgaaaa gcttaaagct ggtgaagtta gcgccgaagg tctgcgcatt
    19621 gtgctggaag cgggtattcg cgagccgatg attatgcggg ccaatcaggc gctgtatgcg
    19681 caattacacc ccataaaaga atccattttc tggcgtcagg ttgacggcgg acatgatgcg
    19741 ctttgttggc gcggtggctt gatgcagggg ctaatcgacc tctggcaacc acttttccat
    19801 gacaggagtt gaatatggca ttcagtaatc ccttcgatga tccacaggga gcgttttaca
    19861 ttttgcgcaa tgcgcagggg caattcagtc tgtggccgca acaatgcgcc ttaccggcag
    19921 gctgggacat tgtgtgtcag ccgcagtcac aggcgtcctg ccagcagtgg ctggaagtcc
    19981 actggcgtac tctgacaccg gccaatttca cccagttgca ggaggcacaa tgagccagca
    20041 tttacctttg gtcgccgcac agcccggcat ctggatggca gaaaaactgt cggatttacc
    20101 ctccgcctgg agcgtggcgc attacgttga gttaactgga gatgttgatg cgccattact
    20161 ggcccgcgcg gtggttgtcg gactagcgca agcagatacg ttgcggatgc gttttacgga
    20221 agataacggc gaagtctggc agtgggtcga tgatgcgctg atattcgaac tgccagaaat
    20281 tatcgaccta cgaaccaata ttgatccgca cggtactgcg caggcattaa tgctggcgga
    20341 tttgcaacaa gatttgcgcg tcgatagcgg taaaccactg gtctttcatc agctgataca
    20401 tgttgcggat aaccgctggt actggtatca gcgttatcac catttgctgg tcgatggctt
    20461 cagtttcccg gccattactc gccagatcgc caatatttac tgcacatggc tgcgtggcga
    20521 accaacgcct gcttcgccgt ttacgccttt cgctgatgta gtggaagagt accagcaata
    20581 ccgcgaaagc gaagcctggc agcgtgatgc ggcattctgg gcagaacagc gtcgtcaact
    20641 gccgccgccc gcgtcacttt ctccggcacc tttagcgggg cgcagcgctt cggcagatat
    20701 tctgcgcctg aaactggaat ttaccgacgg ggaattccgc cagctggcta cgcaactttc
    20761 aggtgtgcag cgtaccgatt tagcccttgc gctggcagcc ttgtggctgg ggcgattgtg
    20821 caatcgcatg gactatgccg ccggatttat ctttatgcgt cgactgggct cggcggcgct
    20881 gacggctagc ggacccgtgc tcaacgtttt accgttgggt attcacattg cggcgcaaga
    20941 aacgctgccg gaactggcaa cccgactggc agcacaactg aaaaaaatgc gtcgtcatca
    21001 acgttacgat gccgaacaaa ttgtccgtga cagcgggcga gcggcaggag aggaaccgct
    21061 gttcggtccg gtactcaata tcaaggtatt tgattaccaa ctggatattc ctggtgttca
    21121 gacgcaaacc cataccctgg caaccggtcc ggttaatgac cttgaactgg ccctgttccc
    21181 ggatgaacac ggtgatttga gtattgagat cctcgccaat aaacagcgtt acgatgagcc
    21241 aacgttaatc cagcatgctg aacgcctgaa aatgctgatt gcccagttcg ccgcggatcc
    21301 ggcgctgttg tgcggcgatg tcgatattat gctgccaggt gagtatgcgc agctggcgca
    21361 gatcaacgcc actcaggttg agattccaga aaccacgctg agcgcgctgg tggcagaaca
    21421 agcggcaaaa acaccggatg ctccggcgct ggcagatgcg cgttacctgt tcagctatcg
    21481 ggaaatgcgc gagcaggtgg tggcgctggc gaatctgctg cgtgagcgcg gcgttaaacc
    21541 aggggacagc gtggcggtgg cactaccgcg ctcggtcttt ttgaccctgg cactccatgc
    21601 aatagttgaa gctggcgcgg cctggctacc gctggatacc ggctatccgg acgatcgcct
    21661 gaaaatgatg ctggaagatg cgcgtccgtc gctgttaatt accaccgacg atcaactgcc
    21721 gcgctttagc gatgttccca atttaacaag cctttgctat aacgccccgc ttacccccca
    21781 gggcagtgcg ccgctgcaac tttcacaacc gcaccacacg gcttatatca tctttacctc
    21841 tggctccacc ggcaggccga aaggggtaat ggtcgggcag acggctatcg ttaaccgcct
    21901 gttatggatg caaaatcact acccgcttac aggcgaagat gtcgttgccc aaaaaacgcc
    21961 gtgcagtttt gatgtctcgg tgtgggagtt tttctggccg tttatcgcag gggcaaaact
    22021 ggtgatggct gaaccggaag cgcaccgtga cccgctcgct atgcagcaat tctttgccga
    22081 atatggcgta acgaccacgc actttgtgcc gtcgatgctg gcggcatttg ttgcatcgct
    22141 gacgccgcaa accgctcgcc agaattgcgc gacgttgaaa caggttttct gtagtggtga
    22201 ggccttaccg gctgatttat gccgcgaatg gcaacagtta accggcgcgc cgttgcataa
    22261 cctgtatggc ccgacggaag cggcggtaga tgtcagctgg tatccggctt ttggcgagga
    22321 actggcacag gtgcgcggca gcagtgtgcc gattggttat ccggtatgga atacgggtct
    22381 gcgtattctt gatgcgatga tgcatccggt gccgccgggt gtggcgggtg atctctatct
    22441 caccggcatc caactggcgc aggggtatct tggacgaccc gatctgaccg ccagccgttt
    22501 tattgccgat ccttttgccc caggtgaacg gatgtatcgt accggagacg tcgcccgctg
    22561 gctggataac ggcgcggtgg agtacctcgg gcgcagtgac gatcagctaa aaattcgcgg
    22621 gcagcgtatc gaactgggcg aaatcgatcg cgtgatgcag gcgctgccgg atgtcaaaca
    22681 agccgttacc cacgcctgtg tgattaacct ggcggcagcc accggtggtg atgcgcgtca
    22741 gttggtgggc tatctggtgt cgcaatcagg tctgccgttg gataccagcg cattacaggc
    22801 acagcttcgc gaaacattgc cgccgcatat ggtgccggtg gttctgctgc aacttccaca
    22861 gttacctctt agcgccaacg gcaagctgga tcgcaaagcc ttaccgttgc ctgaactgaa
    22921 gacacaagcg tcgggacgtg caccgaaagc gggcagtgaa aagattatcg ccgcggcatt
    22981 cgcgtcgttg ctgggttgtg acgtgcagga tgccgatgct gatttctttg cgctgggcgg
    23041 tcattcgcta ctggcaatga aactggcagc gcagttaagt cgacagtttg ctcgtcaggt
    23101 gacgccgggg caggtgatgg tcgcgtcaac cgtcgccaaa ctggcaacga ttattgatgg
    23161 tgaagaggac agctcccggc gcatgggatt cgaaaccatt ctgccgttgc gtgaaggtaa
    23221 tggcccgacg ctgttttgtt tccatccggc gtccggtttt gcctggcagt ttagcgtgct
    23281 ctcgcgttat ctcgatccac aatggtcgat tattggcatt cagtcgccgc gccctcatgg
    23341 acccatgcag acagcgacaa acctggatga agtctgcgaa gcgcatctgg caacgttact
    23401 tgagcaacaa ccgcacggcc cttattacct gctggggtat tcccttggcg gtacgctggc
    23461 gcagggtatt gcggcgcgac tgcgtgcccg tggcgaacag gtggcatttc ttggtttgct
    23521 ggatacctgg ccgccagaga cgcaaaactg gcaggaaaaa gaggctaatg gtctggaccc
    23581 ggaagtgctg gcggagatta accgcgaacg cgaggccttc ctggcggcac agcagggaag
    23641 tacttcaacg gagttgttta ccaccattga aggcaactac gctgatgctg tgcgcttgtt
    23701 aaccaccgcc catagcgttc cgtttgatgg aaaagcaacg ttatttgttg ctgagcgcac
    23761 acttcaggaa ggtatgagtc ccgaacgtgc ctggtcgccg tggattgcgg agctggatat
    23821 ctatcgtcag gattgtgcgc atgtggatat tatctctcca ggggcgtttg taaaaattgg
    23881 gccg
//
LOCUS       DAPHDW010000064        23821 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.064, whole genome shotgun sequence.
ACCESSION   DAPHDW010000064 DAPHDW010000000
VERSION     DAPHDW010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23821
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.064"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            252..4712
                     /gene="gltB"
                     /locus_tag="RH057_002486"
     CDS             252..4712
                     /gene="gltB"
                     /locus_tag="RH057_002486"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HDV3128841.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPHIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHPGKALYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            4725..6143
                     /gene="gltD"
                     /locus_tag="RH057_002487"
     CDS             4725..6143
                     /gene="gltD"
                     /locus_tag="RH057_002487"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HDV3128842.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGSDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            6327..7454
                     /locus_tag="RH057_002488"
     CDS             6327..7454
                     /locus_tag="RH057_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HDV3128843.1"
                     /translation="MESLSERTSTGYQQIHDGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIKRLSKDLSLRYKRGFSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKSFPLPWSTYVRLLSVKNADARSFYE
                     KETLRCGWSVRQLERQIATQFYERTLLSHDKSAMLQQHAPTETHILPQQAIRDPFVLE
                     FLELKDEYSESDFEEALINHLMDFMLELGDDFAFVGRQRRLRIDDNWFRVDLLFFHRR
                     LRCLLIVDLKVGKFSYSDAGQMNMYLNYAKEHWTLPDENPPIGLVLCAEKGAGEAHYA
                     LAGLPNTVLASEYKMQLPDEKRLADELVRTQAVLEEGYRRR"
     gene            complement(7514..7978)
                     /locus_tag="RH057_002489"
     CDS             complement(7514..7978)
                     /locus_tag="RH057_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HDV3128844.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSTIDNEQAIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(7975..8850)
                     /gene="nanK"
                     /locus_tag="RH057_002490"
     CDS             complement(7975..8850)
                     /gene="nanK"
                     /locus_tag="RH057_002490"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HDV3128845.1"
                     /translation="MTTLAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTDLPTIA
                     INDAQAAAWAEYQALEGDVTEMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVETYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(8847..9536)
                     /locus_tag="RH057_002491"
     CDS             complement(8847..9536)
                     /locus_tag="RH057_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HDV3128846.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSDAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(9584..11074)
                     /gene="nanT"
                     /locus_tag="RH057_002492"
     CDS             complement(9584..11074)
                     /gene="nanT"
                     /locus_tag="RH057_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HDV3128847.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(11183..12076)
                     /gene="nanA"
                     /locus_tag="RH057_002493"
     CDS             complement(11183..12076)
                     /gene="nanA"
                     /locus_tag="RH057_002493"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HDV3128848.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGQIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(12198..12989)
                     /gene="nanR"
                     /locus_tag="RH057_002494"
     CDS             complement(12198..12989)
                     /gene="nanR"
                     /locus_tag="RH057_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312126.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HDV3128849.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVADQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
     gene            13369..14736
                     /gene="dcuC"
                     /locus_tag="RH057_002495"
     CDS             13369..14736
                     /gene="dcuC"
                     /locus_tag="RH057_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV3128850.1"
                     /translation="MFGIIISVIVLITMGYLILKNYKPQVVLAAAGIFLMMCGVWLGF
                     GGVLDPAKSSGYLIVDIYNEILRMLSNRIAGLGLSIMAVGGYARYMERTGASRAMVSL
                     LSRPLKLIRSPYIILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAV
                     AVIATTMSIEWGILETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRA
                     FDKKDKNINHEQAELKALDNVPPLYYAILPVMPLILMLGSLFLAHIGLMQSELHLVVV
                     MLLSLTVTMFVEFFRKHNLRETMDDVQAFFDGMGTQFANVVTLVVAGEIFAKGLTTIG
                     TVDAVIRGAEHSGLGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVP
                     AVVMIMPMHFATTLARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIAT
                     ITLFY"
     gene            complement(14779..15276)
                     /gene="sspB"
                     /locus_tag="RH057_002496"
     CDS             complement(14779..15276)
                     /gene="sspB"
                     /locus_tag="RH057_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HDV3128851.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(15282..15920)
                     /gene="sspA"
                     /locus_tag="RH057_002497"
     CDS             complement(15282..15920)
                     /gene="sspA"
                     /locus_tag="RH057_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HDV3128852.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            complement(16315..16707)
                     /gene="rpsI"
                     /locus_tag="RH057_002498"
     CDS             complement(16315..16707)
                     /gene="rpsI"
                     /locus_tag="RH057_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HDV3128853.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            complement(16723..17151)
                     /gene="rplM"
                     /locus_tag="RH057_002499"
     CDS             complement(16723..17151)
                     /gene="rplM"
                     /locus_tag="RH057_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HDV3128854.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            complement(17370..18497)
                     /gene="zapE"
                     /locus_tag="RH057_002500"
     CDS             complement(17370..18497)
                     /gene="zapE"
                     /locus_tag="RH057_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HDV3128855.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSTP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGAKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGDRLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            18691..19089
                     /locus_tag="RH057_002501"
     CDS             18691..19089
                     /locus_tag="RH057_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HDV3128856.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQMPRDYSEGASGLLRTGAKRD"
     gene            19243..20610
                     /gene="degQ"
                     /locus_tag="RH057_002502"
     CDS             19243..20610
                     /gene="degQ"
                     /locus_tag="RH057_002502"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
                     /protein_id="HDV3128857.1"
                     /translation="MKKQTQLLSALALSVGLTLSASFQAVASIPGQVADQAPLPSLAP
                     MLEKVLPAVVSVRVEGTASQGQKIPEEFKKFFGDDLPDQPAQPFEGLGSGVIINASKG
                     YVLTNNHVINQAQKISIQLNDGREFDAKLIGSDDQSDIALLQIQNPSKLTQIAIADSD
                     KLRVGDFAVAVGNPFGLGQTATSGIVSALGRSGLNLEGLENFIQTDASINRGNSGGAL
                     LNLNGELIGINTAILAPGGGSVGIGFAIPSNMARTLAQQLIDFGEIKRGLLGIKGTEM
                     SADIAKAFNLDVQRGAFVSEVLPGSGSAKAGVKAGDIITSLNGKPLNSFAELRSRIAT
                     TEPGTKVKLGLLRNGKPLEVEVTLDTSTSSSASAEMITPALEGATLSDGQLKDGGKGI
                     KIDEVVKGSPAAQAGLQKDDVIIGVNRDRVNSIAEMRKVLAAKPAIIALQIVRGNESI
                     YLLMR"
     gene            20700..21767
                     /gene="degS"
                     /locus_tag="RH057_002503"
     CDS             20700..21767
                     /gene="degS"
                     /locus_tag="RH057_002503"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HDV3128858.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(21830..22768)
                     /gene="mdh"
                     /locus_tag="RH057_002504"
     CDS             complement(21830..22768)
                     /gene="mdh"
                     /locus_tag="RH057_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV3128859.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVSKTCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQSALEGMLDTLKKDIALGEEFVNK"
     gene            23203..23673
                     /gene="argR"
                     /locus_tag="RH057_002505"
     CDS             23203..23673
                     /gene="argR"
                     /locus_tag="RH057_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HDV3128860.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
BASE COUNT         5765 a         6336 c         6188 g         5532 t
ORIGIN      
        1 atttgacctg tatcagcttt cccgataagt tggaaatccg ctggaagctt tctggatgag
       61 cagcctgctc atcatattta tgcagtaatt gagatcccct cttcaccgta ttaaccgatg
      121 cgaaaaggac aacaaggggg cgaatgcgag gcgcgcgtat gacacgcaaa ccccgtcgcc
      181 acgctctttc tgtgcccgtg cgcagcggtt cggaagtggg gttcccgcag agcctggggg
      241 aggttcacga tatgttgtac gataaatccc ttgagaggga taactgtggt ttcggcctga
      301 tcgcccacat agaaggcgaa cctagccaca aggtagtgcg tactgcaata cacgcactgg
      361 cccgcatgca gcaccgtggc gcgattctcg ccgatggtaa aaccggcgac ggttgcggct
      421 tgctgttaca aaaaccggat cgcttttttc gcatcgttgc gcaggagcgc ggctggcgtt
      481 tagcaaaaaa ctacgctgtc gggatgctct tcctgaataa agatcctgaa ctcgccgctg
      541 ccgcacgccg catcgttgaa gaagagctgc aacgcgaaac cttgtcgatt gtgggctggc
      601 gtgatgtccc cactaacgaa ggcgtgctgg gtgaaatcgc cctctcctct ctgccccaca
      661 ttgagcaaat tttcgttaac gctccggcag gctggcgtcc gcgcgatatg gagcgccgtc
      721 tgtttatcgc ccgccgccgc attgaaaagc gtctcgaagc cgacaaagac ttctacgtct
      781 gtagcctgtc gaatctggtg aacatctata aaggtctgtg tatgccggcg gatctgccgc
      841 gcttttatct ggatcttgcg gacctgcgtc tggaatcggc catctgcctg ttccaccagc
      901 gcttctccac taacaccgta ccgcgctggc cgctggcgca gccgttccgc tacctggcgc
      961 ataacggtga aatcaacacc atcaccggta accgccaatg ggcgcgtgcg cgtacctata
     1021 aattccagac gccgctgatc cctgatctgc acgacgccgc accgttcgtc aacgaaaccg
     1081 gttctgactc cagttcgatg gataacatgc tggaactgct gctggcaggc gggatggata
     1141 tcatccgcgc catgcgtcta ttagtaccac ccgcctggca gaacaacccg gatatggacc
     1201 cggaactgcg tgccttcttt gactttaact ccatgcatat ggagccgtgg gatggcccgg
     1261 cgggcatcgt gatgtccgat ggtcgcttcg ctgcctgtaa cctcgaccgt aacggtctgc
     1321 gtccggcacg ctacgtcatt accaaagaca agctcatcac ctgcgcctct gaagtcggta
     1381 tctgggatta tcagcctgac gaagtggtcg aaaaaggccg cgtcgggcca ggcgaactga
     1441 tggtgatcga cacccgcagt gggcgtattc tgcactcggc agaaaccgat gacgatctga
     1501 aaagccgcca tccatataaa gagtggatgg agaaaaacgt ccgccgactg gtaccgtttg
     1561 aagatctgcc cgatgaagaa gtgggtagcc gcgaactgga cgacgacacg cttgccagct
     1621 accagaaaca gtttaactac agcgcggaag agctggactc cgtaattcgc gtactgggcg
     1681 aaaacggtca ggaagcggtc ggttcgatgg gcgatgatac cccattcgcc gtgctctcca
     1741 gtcagccgcg cattatttac gactacttcc gccagcagtt tgcccaggtg actaacccgc
     1801 caatcgaccc gctgcgtgaa gcgcatgtta tgtcgctcgc caccagtatc ggtcgtgaaa
     1861 tgaacgtctt ttgcgaagca gagggccagg cgcaccgttt aagctttaaa tcgccgattc
     1921 tgctctactc cgatttcaaa cagctcacga cgatgaaaga ggagcactac cgcgcagata
     1981 cgctggatat cacctttgac gtcactaaaa ccacgctcga agcgacagtc aaagagctgt
     2041 gcgacaaagc cgaaaaaatg gtacgtagcg gcaccgtgct gctggtgctc tccgaccgga
     2101 atatcgctaa agatcgcctg ccggttccag ccccgatggc ggttggcgcg atccagaccc
     2161 gtctggtcga tcaaagcctg cgttgcgatg ccaacatcat cgtcgaaacc gccagcgccc
     2221 gcgatccgca ccacttcgcc gtgttgctgg gcttcggcgc gacggctatt tatccatacc
     2281 ttgcctatga aacgctgggc cgcctggtag acacccatgc gattgccaaa gattatcgta
     2341 ccgtgatgct caactaccgt aacggcatca acaaaggctt gtacaaaatc atgtccaaaa
     2401 tgggcatctc caccatcgcc tcttaccgct gctcgaaact gtttgaagcg gtcggtctgc
     2461 acgatgatgt agtgggcctg tgcttccagg gggcggtcag ccgcattggt ggggcgagct
     2521 ttgaagactt ccagcaggat ctgctgaacc tgtcgaaacg tgcctggctg gcgcgtaagc
     2581 ccatcagcca gggcggcctg ctgaaatacg tccacggcgg cgaataccac gcttacaacc
     2641 cggacgtggt gcgcacgctg caacaggcgg tacaaagcgg cgagtacagc gactatcagg
     2701 aatacgcgaa gctggttaat gagcgtccgg caaccacgct gcgcgatctg ctggcaatta
     2761 cgccgggtga aaacgcggtc aacattgctg atgttgaacc ggcaagcgaa ctgtttaaac
     2821 gctttgatac cgccgcgatg tctatcggcg cgttaagccc ggaagcccac gaggcgctgg
     2881 cggaagcgat gaatagcatc ggcggtaact cgaactccgg tgaaggcggc gaagacccgg
     2941 cgcgctatgg cactaacaaa gtgtcgcgca tcaagcaggt ggcttctggt cgcttcggcg
     3001 ttactccggc gtatctggtc aatgccgacg tcattcagat taaagtcgcc cagggcgcga
     3061 agccgggcga aggcggtcag ttgccgggcg acaaagtcac tccttacatc gccaaactgc
     3121 gctattcggt gcccggagtg acgctgatct ccccgccgcc gcaccacgat atctactcta
     3181 tcgaggactt agcgcagctc attttcgacc tcaagcaggt taacccgaaa gcgatgatct
     3241 ccgtgaagct ggtttccgaa ccgggcgtag gcaccatcgc gactggcgtg gcaaaagctt
     3301 atgccgattt aatcaccatc gcaggctatg acggtggcac cggcgcaagc ccgctgtcgt
     3361 cagtgaaata cgcaggttgc ccgtgggagc tggggcttgt tgaaacccag caggcgctgg
     3421 ttgccaatgg tctgcgtcat aagatccgtt tgcaggtcga tggcggcctg aaaaccggtg
     3481 ttgatatcat caaagcggcg attctcggcg cagaaagctt cggcttcggc actggcccga
     3541 tggtggcgct cggctgtaaa tatctacgta tttgccatct gaacaactgc gcaacgggtg
     3601 tagcaactca ggatgacaaa ctgcgtaaga accactatca cggcctgccg ttcaaggtta
     3661 ccaattactt tgaatttatt gcccgtgaaa cccgcgagct gatggcacag ctgggcgtaa
     3721 cgcgtctggt ggatctgatt ggtcgcaccg acctgctgaa agaactggac ggtttcaccg
     3781 ccaaacagca aaaactggcg ctgtcgaagc tgctggagac cgccgaaccg catccaggta
     3841 aggcactcta ctgcaccgaa aacaacccac cgtttgataa cggcctgctg aacgcgcagt
     3901 tgctgcaaca ggcgaaaccg tttgtcgatg aacgccagag caaaaccttc tggttcgata
     3961 ttcgcaacac cgaccgttct gtcggcgcgt cgctttcagg ctatatcgcc cagacgcacg
     4021 gcgatcaggg gctggcagcc gatcctatca aagcgtactt caacggcacc gcaggccaga
     4081 gtttcggtgt gtggaacgcg ggcggcgtgg aactgtacct gaccggggat gccaacgact
     4141 atgtcggtaa aggcatggcg ggcggcttaa tcgccattcg tcctccggtt ggttccgcct
     4201 tccgcagcca tgaagcaagt attatcggca acacctgcct gtatggcgcg accggtggtc
     4261 gtctgtatgc cgcaggccgc gcgggtgaac gtttcggcgt gcggaactcc ggtgctatca
     4321 ccgtggtaga aggcattggc gacaacggct gtgaatatat gacgggcggt attgtctgta
     4381 ttctgggtaa aaccggcgtt aacttcggtg cgggcatgac cggcggcttc gcttacgttc
     4441 tcgatgaaag cggtgatttc cgcaaacgcg ttaacccgga actggtcgag gtcttaagcg
     4501 ttgacgatct ggcgatccat gaagagcatc tgcgcggtct tatcaccgag catgttcagc
     4561 atactggctc tcagcgcggt gaagagattc tggcgaactg gtcaaccttc gccactaaat
     4621 ttgcgctggt taaaccgaag tccagtgatg taaaagcact gctgggtcat cgtagtcgta
     4681 gcgcagcaga gttgcgcgtg caggcgcagt aaggggtagc aacaatgagt cagaatgttt
     4741 atcaatttat cgacctgcag cgcgttgatc cgccaaagaa accgctgaag atccgcaaaa
     4801 ttgagtttgt tgaaatttac gagccgtttt ccgaaggcca ggccaaagcg caggctgacc
     4861 gctgcctgtc gtgcggcaac ccatactgcg agtggaaatg tccggtacac aactacatcc
     4921 cgaactggct gaagctcgcc aacgaggggc gtatttttga agcggcggaa ctgtcgcacc
     4981 agaccaacac cctgccggaa gtttgcggac gagtctgccc gcaagaccgt ctgtgcgaag
     5041 gttcctgcac tctgaacgat gagtttggcg cggtgaccat cggcaacatt gagcgttata
     5101 tcaatgataa agcgttcgaa atgggctggc gtccggatat gtccggcgtg aagcaaaccg
     5161 gtaaaaaagt ggcgattatc ggcgcaggcc cggcaggtct ggcgtgtgcg gatgtcctga
     5221 cgcgtaacgg cgtaaaagcc gttgtcttcg accgtcatcc agaaattggc gggctgctga
     5281 ccttcggtat tccggccttc aagctggaaa aagaggtaat gacgcgtcgc cgtgaaatct
     5341 tcaccggcat gggtattgaa ttcaaactca ataccgaagt gggccgcgac gtgcagctgg
     5401 acgatctgct gagtgattac gatgccgtct tccttggcgt cgggacttat cagtcaatgc
     5461 gcggcgggct ggaaaacgaa gacgccgatg gcgtgtacgc agcgctgccg ttccttatcg
     5521 ccaacaccaa acagttaatg ggctttggtg aaacccgcga cgaaccgttc gtcagcatgg
     5581 aaggcaaacg cgtggtggtc cttggcggtg gcgacactgc gatggactgc gtgcgtacgt
     5641 ccgtgcgcca gggagcgaag cacgttacct gtgcctatcg tcgtgatgaa gagaacatgc
     5701 cgggttcccg ccgcgaagtg aaaaacgcgc gggaagaagg cgtagagttc aaattcaacg
     5761 tccagccact ggggattgaa gtgaacggta acggtaaagt cagcggcgta aaaatggtac
     5821 gtaccgaaat gggcgaaccg gatgccaaag gccgtcgccg cgcggagatc gttgcaggtt
     5881 ccgaacatat cgttccggca gatgcagtga tcatggcgtt tggtttccgt ccacacaaca
     5941 tggaatggct ggcaaaacac agcgtcgagc tggattcaca aggccgcatc atcgccccgg
     6001 aaggcagcga caacgccttc cagaccagca acccgaaaat ctttgctggc ggcgatatcg
     6061 tccgtggttc cgatctggtg gtaaccgcta ttgccgaagg tcgtaaggcg gcagacggta
     6121 ttatgaactg gctggaagtt taagctctgt cccagcgccg cctcctcaga aaagagtcgg
     6181 cgctgaaatt ctcctttctt tctggaaagc gcctcgcata atctgaagaa aaccaatgta
     6241 atacgctcat ctggattgtt accggtagat gcccggcgta tagctgattc cattattcaa
     6301 cgcagcgcca tataaaggga cagataatgg aatcgctctc agagagaacc tcaacaggct
     6361 accagcaaat ccacgacggt attattcatc tggtcgatag cgcccggacg gaaacggtac
     6421 gtagcgttaa cgcgctgatg accgcgacat actgggaaat tggccgacga attgtcgaat
     6481 ttgaacaagg tggcgaggcc agggctgcgt atggtgcgca gctaatcaag cgactatcaa
     6541 aggatttaag tctaaggtat aagcgtgggt tctctgcaaa aaacttacgc caaatgaggc
     6601 ttttttacct cttttttcaa catgttgaaa ttcgccagac agtgtctggc gaattaacac
     6661 cattgggaat tccgcagaca ccatctgcag aattcccatc ggcaaagatt tggcagacgc
     6721 tgtctgccaa atctttcccc ctgccctggt ccacctacgt ccgtttactt tccgttaaaa
     6781 acgctgacgc ccgcagcttt tatgaaaaag agacgctccg ctgtggctgg tctgttcgcc
     6841 agctagaacg gcaaattgcg acccaatttt atgagcgaac actactgtca catgacaaat
     6901 cagccatgct gcaacaacac gctcctaccg agacgcatat tcttccgcaa caggcgatac
     6961 gcgatccctt tgtgctcgaa tttctggaat tgaaagatga atattcagaa tctgatttcg
     7021 aggaggcgct gatcaaccac ctgatggatt tcatgctgga acttggggat gattttgcct
     7081 ttgttggtcg gcagcgaaga ttacgcattg atgacaactg gtttcgtgtc gatctgctgt
     7141 ttttccaccg ccgtttacgc tgcctactaa tcgtcgatct aaaagtgggc aaattcagct
     7201 atagcgatgc cggacagatg aatatgtatc tcaactacgc caaagagcac tggacgctac
     7261 cggatgaaaa tccgcccatc ggtctggttc tctgtgcaga gaaaggagcc ggagaagcac
     7321 attatgctct ggcaggtttg cctaacaccg ttctggcaag cgaatataag atgcaactac
     7381 ctgatgagaa acgactcgca gatgaactcg ttcgaacaca ggcggtgcta gaggaaggct
     7441 atagacgccg ttaatttcag cggtttcgaa ttctgcagac agtgtctgca gaatctttaa
     7501 aacgaactaa gttttaagcc attaaatcag ccttaacctt caccacaacc tttttaatct
     7561 ctccaggctc gccgacaacg catcctggtt tatgcggttc acctggcata aacacggcga
     7621 acattcccgg ttttaagatg atggcttgct cgttatcaat ggtgctgcaa agctgataat
     7681 catcctcatg gtggaactct tcacactggc gcgcagtgcc tgccatgcca aacagaatcc
     7741 gttcctcacc gtttaataac agttggatat caatgtattg ctcgtgcaat tccgcttttt
     7801 tctcgacggg cgattgagtg ttaaacgtca tgacattcat aaagatattg tcgccctgta
     7861 attcgtaacg acccggcgct ttttcttgcg gtctggcagc taatgccagc gttaacgcgt
     7921 cctgtaacgc aggatgtaac ccagcagacg gtaatgactg tacttcaccc atcatcataa
     7981 tttttctccc tgggccaaca gcgcagcccc aagtaaacct gcatcgtggc ggtagtgcgc
     8041 cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc agatacgttt ccaccagcgc
     8101 cagataccct tctgccagcc caacgctgcc accgaccacc acgcactggc aatcagttgt
     8161 ggctttaata tcagcgatca gccttgcaag cgtacgtgcg gagcggtgaa tcagctgctg
     8221 cgcctgctcg tcgccctgcc cggcgcgtgt gaaaatagtt ctcgcatccg cgcccgccag
     8281 ttcaccctgc gctgccgccg caatgccgcg accagaagca atcgcctcca cgcaacctgt
     8341 gcgtccacag ccacagaccg ggccgtgtgg atcggcaagc gtatgcccga tatgccccgc
     8401 cagaccgcca gggccggtaa gcagtttgcc gccgctcact acaccgccgc cgacgccagt
     8461 ggaaacggtg ataaacacca tctcggttac atcgccttcc agcgcctgat actccgccca
     8521 tgctgcagcc tgcgcgtcgt taatggcaat ggtcggcaaa tcggtaagtt gttccagcgt
     8581 tttgactaac ggaaagtgta gcaatccacc aagattatgc ggattcagcg ccagcaagct
     8641 gccgtcgcga ataatcccgg ttgaagcgat ggcaacccgc tgcgcatgag cttgcaacgg
     8701 agagactaat gcggataagg catcacgcaa ggcttctggt gtctggctgg ctggcgtagg
     8761 aagttcacga cgatcgcgga tctgcccgtc agcgccaatc agcgcggcgg caagtttagt
     8821 accgccgata tcaatcgcca gcgtggtcat agcaccgcct ttttcatcgc tgtgttgtac
     8881 cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga ccgtcaccgc ccacgcgccg
     8941 tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac gcccttcggc aatcacccta
     9001 catccggcgt cgctcaacgt tttcaccagc gccagatccg gctcttctgg cgtttcaggc
     9061 gtggtatagc cagaaagcgt agtgccaata atttcggctc ccagcttttg gcatgccagg
     9121 ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac cgtgatggtg aatacgtgcc
     9181 agcagcgttt caacaggcac cggacgcggg cggtcggtgc cgtcaatggc gataatgtcc
     9241 gcgcccgcct gcgccagcgc atcgacatct tcaatatagg ccgtgatgcg taccggagaa
     9301 tcctccagat cgcgtttcac aatcccgata attggcacgc tcaccaccgc acgcgtggct
     9361 tgcagatttg ccacaccttc aatgcgaatg gcaaccgcgc ccgcctgctc tgccgctaat
     9421 gccatggcgg cgacgatttc gggtttatcg agcggactgt ccggaaccgg ctggcaggag
     9481 acaatcaggc caccgttagc agcgattttt tgatccagtt gtgcaagtaa cgacatacat
     9541 cttcccttag cgaaaggccc ggtacataga ccgggcaaca ggattaactt ttggttttga
     9601 ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct aaatggtttg ccgtcgatag
     9661 cgtcatgagt acgcaatgct tccgggcgca gccaacgctg aacgcgggaa ggcatatcca
     9721 gaccaatcag caggatcacc acgaacgtca gactgaacga gagcgatgcc agcgcagtac
     9781 ccagatccag acgttgagcg atcaacgcgc cgatgattgg ggccagtgcg ccgcccaatg
     9841 cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg gtcggtatcg aaataaccgc
     9901 caatcagttt cggtaagatc ccggcgatcc cttgtccaag catttgctgg aagaacagta
     9961 acagaccgag cacccagacg tttgcgccgc caatcgcaaa taccggaata atcagcagct
    10021 gcgaggccag caggctacaa acgtacgctt tgcgggttcc cagccagtca ccgaggaagc
    10081 cacctacgca gcatcccacg gccgcgccaa agccactaaa gaacagcaca ttggcgacag
    10141 tatgcgggtt ataagccaga tcggttttca gatacgttgg cagcagcgcc tgaatcggcc
    10201 atgagtagag gaaagcaaac aacacgacca ccatcagcat tacgcccgtt ggccagcgtt
    10261 tacccgtact ttgcaccata aagctgataa agattgcggc gcataacagc ccgagaacag
    10321 cgacgattgc agcattttgc aggttaccgg cgaagcagaa ccacagcgca gtagccgccg
    10381 ccagtgtcat tacgatattg gcaatgcgat gttcaccacg gtagagaata tccaccattg
    10441 tgcgtactgg tgctttacct gcgtgtttct ctttccagtc ttccgcttcc gggatgtttt
    10501 tacgcagcca gagagcaaag atgattggca aaatgccgat aaagaacagc gcacgccaac
    10561 cccagaccgg aaccaccagg ctatagacct gagcggcaac gacggccccc acagagaagc
    10621 ctgaaatcaa aaaaccactg gctttgttac gcagatgttt tggccagctt tcaatgacat
    10681 aggtggcgct ggaaccgtat tcacccgcca tccccatgcc gatgaccaga cgagcgataa
    10741 acatggtgat gtagcctggc gcaaagccgc aggccagcgt cccggccgag aagagaacga
    10801 tgctggtgac cattgccaga cgacgcccgt agcggtcacc catagcgccg agcatcaggc
    10861 cgccgaacca gcgagagata aaggctgcgg agatcagact tgccgcctgc accgtcgtca
    10921 gcccgaattc accctgtact tcggtgagta ccagggcgat taaaacgaaa tcaaaaccgt
    10981 caagcagata tcccaaccag gcagcggaaa atgcccgcca ttgtgcacga ttgagatggc
    11041 gataccacgg gatattctgg gttgtagtac tcattgtgag tctcccgcgg tgggcgatgc
    11101 ccacacgctt tggtatgaaa attgtagggt acagatgcgt ttatttcccc tcacccggta
    11161 ggggcgagcg aggggaaaca actcacccgc gctcttgcat caactgctgg gccagcgcct
    11221 tcagttctgg cagatatttt tcatctaccg gaccaaacgg tttgcggcac agcggcacag
    11281 aaacgacatc catataatgc aggacagttt tcaggccgcg gaatacgccc gttttgatca
    11341 gtaaatcaat gactttatta cattcggttt gcagcttctg cgcggtctgg atatcgcctt
    11401 ctttcagcgc cttcacaatc ccctgataac gccagcccat gatgttgtag gtactaccga
    11461 taccaccatc agcgcccgcc agaagaccag aggcgaagat ttcgtcgtaa ccgttataga
    11521 gcaccagatc aggatgttca cgacggatct gctccatctg atagagatcg ccagaggtct
    11581 gtttcagcgc acctacgcca ggcaacgtaa caagtgtgtt gatctgatcc agggtcagtt
    11641 ttaccccact cagggctgga atgttgtaca ccaccatcgg caaaccatcc gccgaatcaa
    11701 taattgcccg atagtgatcg cagtgttctt caaagctgaa aggatagtag aacggcgtga
    11761 cggcggagac ggcatcgaag ccataacgtt tagccgatgc cgcaagttgt tggctttcgg
    11821 cggtgctgac gcaaccgacg tgggcgatga gtttaatctg acctttcgcc tcttcggcga
    11881 cgatttccag tacctgttca cgctcggaaa ggctttgtac aaaggcctcg ccggtcgaac
    11941 cacccacgta taaaccgtcg atgccctgct gaatattgaa ctgaaccagg cgacgcagac
    12001 tcgctttatc cagtgcttgt tgttggtcaa aaggagtcag gagtgcagcc attacgccac
    12061 gtaaattcgt tgccataaat acctctgaag tgatgcttgt ctgataaacg atataccttt
    12121 atacctgtta taccagatca attaagcaac accccataca gaaagcttat aatgcgatct
    12181 gcttcactaa agtggcatta tttctttttg ttggtggtct gaccgaaggc gtgccaggta
    12241 gcagagacgc tgttgagatg cgattgcaac gcacgatcgg cttcgtcagg atcgtggcgg
    12301 cggatcgcgt caacgatcgc aatatgctgt tgatagctaa cgttgttatg ctcgtgcaga
    12361 gcctgatcag ccacggttgg acgtgcggcg attagccagt cgagcagggc aacgtggatc
    12421 gccatgaaga ttgggttacc tgggatctcc gccagcacgc ggtggaaatc aacgtctgaa
    12481 cgaatgaatg ccgcgttgtt atccagcgac tgactgttga tttccagtgc ttttgccagc
    12541 aaatcgattt gctcatcggt ggcgtgttca gccgcatagc gcaccaggct ggattcaaag
    12601 aacagacgta attgttcgaa atgggcaatc ccaccaggat gagaaaggaa atctttcgcc
    12661 atgccggaaa gctcaccgat gatagtgtcc gcagaaggac gcgagacgcg agcgcgttcg
    12721 ccgttgttta tttgcaccag acctttgcgt tttaacgctg ccagcgcttc gcgcacagaa
    12781 ggacgcccga cgttaaagaa cgccatcagt tcgcgttcag acggtaattg ttcaccttcg
    12841 ccaaattcac gacggcggat catctgttcc agctcttctt ccaccatttc ggagagtttt
    12901 ttacgcgcca gcgggcggct acgcaagttg cgaccaattg caggtgaaga atcttcggtt
    12961 tgcgaatcaa atgcgttcat aaggcccatt ctgtaaggtc agtgtgatta acatcatcag
    13021 tgacatccta tcacaggatt gaaagtaggg gaaaatggca ggtttttctc tttgtgcctc
    13081 atcattacca taattaacgg aataattaac tattgcgaaa aattaatgta acgcagataa
    13141 aaacatcccg tttgaattat tcataagact attcacgagc attatgaata ttatgaatgt
    13201 gttcttacaa aataatcata agcgcatatt ttttaatgaa aaaccacctc acctacaatt
    13261 aaaaacacga catccgcacc ataaatagac ttgcaaaaaa tataacatca ttgttttcaa
    13321 tctgccgttt atgggattga ccgttttctt ttgacacgga gttcaacaat gttcggcata
    13381 attatatctg tcatcgtatt aattacgatg ggctatttga tcctgaaaaa ctacaaacct
    13441 caggtggtgc tggctgccgc aggtatcttc ctgatgatgt gcggtgtctg gttagggttc
    13501 ggtggtgtac tcgatcccgc caaaagcagc ggctacttga tcgtcgatat ttataatgaa
    13561 atcctgcgca tgctgtccaa ccgcattgcc ggattggggc tgtcgattat ggcggtgggc
    13621 ggttatgccc gctacatgga gcgcacagga gccagtcgcg cgatggtgag cttgctaagc
    13681 cgcccgttaa aactcattcg ctcgccgtat attattctgt cggcaactta cgtcatcggc
    13741 caaatcatgg cgcagtttat taccagcgcc tccggcctgg gtatgttgct gatggtcacc
    13801 ttatttccga cgctggtgag tctgggagta agtcgcctct ctgcggtggc ggttatcgca
    13861 accacgatgt ccattgagtg ggggattctg gaaacgaact ccatttttgc agcccaggtc
    13921 gcgggaatga aaattgccac ttacttcttc cactaccagc ttccggtcgc ctcttgcgtc
    13981 attatctcgg tggcgatctc ccactttttc gtgcaacgcg cttttgacaa aaaagataaa
    14041 aatatcaatc acgaacaggc agagctaaaa gctctcgata atgtcccgcc gctctattac
    14101 gccattttac ctgtgatgcc gttaatcttg atgctcggct cgctgttcct cgcccacatc
    14161 gggctgatgc agtcagaact gcatctggtg gtggtgatgt tactgagttt gacggtgacg
    14221 atgtttgttg agttcttccg caagcataac ttgcgcgaaa caatggacga tgtgcaggcg
    14281 ttttttgacg gcatgggtac gcagtttgcc aacgtggtaa cgctggtggt cgcgggtgaa
    14341 atatttgcga aaggcttaac gacgattggc actgttgatg cggttatcag gggtgcggag
    14401 cattctggtc tgggcggtat tggcgtgatg attattatgg cgctagtcat tgccatttgt
    14461 gccattgtga tgggctctgg caatgcgccg tttatgtcat ttgccagtct tattccgaat
    14521 atcgcagccg gactacatgt accagcggtt gtaatgatta tgccgatgca ttttgccacg
    14581 acgctagcgc gcgcggtttc gccgattact gcggtggtgg tcgttacgtc aggaattgca
    14641 ggcgtttcgc cttttgcggt ggtgaagcga acagcgatcc ccatggcagt cggtttcgtg
    14701 gtgaatatga ttgccacaat cacgctattt tattaagcca ttaaaagaca aaacaggcca
    14761 ccagggcctg tttttgtatt acttcacaac gcgtaatgcc ggtcgaccac cgcgtggtgg
    14821 ctgcggaggt tcatcgtcag gatgagtgtc atcatcgtga tctggcttgt cgccatcaat
    14881 aaccgacata acggtttcgt tgtctgccga tgcctcttca tcattcatga tgctggtatc
    14941 ttcatcgtag gcagcttcag gctcaaacat cgtgcctgcg ccattttcac gggcgtagat
    15001 agccagcacg gcagccagcg gcacagaaac ctgacgcgga atgccgccaa agcgcgcgtt
    15061 aaagcgtacc tcatcattcg ccagttccag attgccgaca gcacgcggcg caatgttgag
    15121 tacgatttgc ccgtcacgcg catattccat aggaacctgc acgccaggga gcgtcacatc
    15181 caccaccaag tgcggcgtga gctggttatc cagcaaccac tcatagaatg cacgcagcag
    15241 atagggacga cgtggtgtta gctgtgacaa atccatacag attaactccg gcccagacgc
    15301 atttcacgtt ctgcttcagt taaagaagca aggaaagagt cacgctcaaa gacgcgggtc
    15361 atatagcctt tcagctcttt cgcacccggg ccgctgaact cgatgcccag ttgcggcaga
    15421 cgccacagca gcggagcaag atagcaatcg accaggctga actcatcgct caggaagtac
    15481 ggcttctgac cgaagaccgg cgcaatcgcc agcagttctt cgcgcagttg cttacgtgcg
    15541 gcatctgctt cagaagctga accgttgatg atggtgttca tcagcgtgta ccagtctttt
    15601 tcgatgcgat gcatgtacag acggctttca ccgcgagcta ccgggtaaac aggcatcagt
    15661 ggcggatgcg ggaaacgctc atccagatat tccataatga tgcgagattc ccacagggtc
    15721 agctcacgat ccaccagggt cggaacgctc tgattcgggt tgaggtcaat cagatcctga
    15781 ggcggattgt ccttttccac gtgttcgatc tcgaaactta cacctttctc agccagcaca
    15841 atgcggacct gatggctata gatgtcagta ggaccggaaa acagcgtcat taccgaacgt
    15901 ttgttggcag cgacagccat gaaaacctcc aggtatagtc agaattttta ctgctaccag
    15961 ccaccaggtg gccagtcaga agttgtgtta cccagtaagg aacgactctc tttgttcgaa
    16021 aatcaaacaa aaaatgagca atacccgaca tttgggcaga aaattggatg atagtttacc
    16081 agattttgcg acctttgtgg tgagtcgatg ccggaaatgg ggaaaaagag atgcgcttta
    16141 gtctgaaata gttgacttag tcccttattg gcgatgtggt ttttgttttg cctgtctgtc
    16201 agacggtagc aaaaagcaac tttccaattt ttacgctgat tcagaattta accataaaaa
    16261 aacccgccga agcgggtttt ttcgcaaatt gttttctgcc ggagcagaag ccaattaacg
    16321 tttggagaac tgcggacgac gacgtgcttt acgcagaccg actttcttac gttcaacctg
    16381 acgagcgtca cgagtaacga agccagcttt acgcagttca gaacgcaggg actcgtcgta
    16441 ttccatcaga gcgcgggtga taccgtgacg gatcgcacca gcctgaccag agataccacc
    16501 acctttaacg gtgatgtaca ggtccagttt ctcaaccatg tcgaccagtt ccagcggctg
    16561 acgaactacc atgcgagcag tttcacgacc gaagtactgt tccagagaac gttggttgat
    16621 tacgatttta ccgttgcccg gtttgatgaa aacgcgagct gcggaacttt tgcggcgacc
    16681 agtgccgtag tattgatttt cagccattgc ctataatccc gattagatgt caagaacttg
    16741 cggttgctgt gccgcgtggt tgtgctcgtt acccgcgtaa actttcagtt tacggaacat
    16801 agcacgaccc agcgggcctt ttggcaacat gcctttaacc gcgatttcaa tcacacgctc
    16861 aggacggcga gcaatcatct cttcaaaggt cgcttgtttg ataccaccga tgtggccggt
    16921 gtggtgatag tacactttgt cagtacgctt gttgccggtt acagcaactt tgtcagcgtt
    16981 cagaacgatg atgtaatcac cggtatctac gtgcggagtg tattccgctt tgtgcttacc
    17041 gcgcaggcga cgagccagtt cagtagccag acggcccaga gttttaccgg tcgcgtcaac
    17101 aacataccag tcgcgtttta cggtttctgg tttagctgta aaagttttca ttaaaagctt
    17161 acccaataaa tagttacacg ttggtgaaca cccaaacgtc ttcaattgtt gaggttcaca
    17221 cgacaaagtc cggcaaacct accccttcga atagcctatg ccagcacaca aaaagttttg
    17281 ggaaaaaaac tttcttgtaa cgtggggtcg caggattata gagaagtcgg ggtcaaagat
    17341 cgaccccttt ttgtgatttg tgacaggttt taacccgcca aatgctcgcg cttcagatac
    17401 tcttcgcttt gcatctcttg cagacgtgac aggcaacgct ggaactcaaa cttcagccga
    17461 tcgccctgat aaatttcata cagcggcact tctgcactca ccactaattt gacatggcgc
    17521 tcgtaaaact catccaccag cgcaataaag cgccgcgctt cgctctccat caaccgcgtc
    17581 ataactggta catcaaacaa catgaccgta tgaaagagac gtgagagcgc aatatagtca
    17641 tgctgactgc gggcgtcgac gcacagcgta gtaaaagaga ccgccagcgt ctggttctcg
    17701 acgcccattg tcgccaatgg ccgatggttg atttctaacg tcggtgaatt ttctcgtttc
    17761 gcccccgcca gcgccaacca tagtttatcc atctgcgccc gggtttcatc gttgagtggc
    17821 gaaagccaca gatgcgcctg agtgagtgta cgcagtcgat aatcaacacc agcgtccacg
    17881 ttcattacat cacaatgctg tttaatggca tcgattgcag gcagaaaacg cgcacgttgc
    17941 aggccatttc gataaagttc gtccggcgga atatttgacg tcgctaccag ggtaataccg
    18001 cgggtgaaca gggctttcat cagaccgcca agtagcatgg cgtcggtaat atcagaaaca
    18061 aaaaattcgt caaaacagag cacgtcagtt tcggctttaa agcgatcggc aataatttcc
    18121 agcggatcgg tctgcccctg taaggcagtt agctcttcat gcacccgcag cataaaacgg
    18181 tgaaagtgca ggcgctgttt ccgctctccc ggcaggcttt gatagaaaag gtccatcagc
    18241 caggttttcc cgcgtcctac accgccccac atatataagc cacgcactgg cgtatgcttt
    18301 gtgtcttcgc gtttacccca cagcttaccg acccgcgcca ttagcccact cgtcctgggg
    18361 gctggtggcg tgctattgat gagttcctga taaataattt ccaggcggct gacggcctct
    18421 ttttgaacgt cgtcgggttg atggctgcct tcattgagcg ccttcaggta ttgcgatgtt
    18481 ggggtaacgc tttgcatgat cttattgtta ttccttgaat aatcggtgcg ccgttgttca
    18541 cggttgacga aaaaaaggcc gttctacact acgcgatatg cagtcgggat tccacttctg
    18601 tggaattaac ggttatagtg gcataatcag ccgcaggcat ggagcctgaa gccaacaccc
    18661 tacggaaaca aaagacaacg ggagatgttc atgacctggg aatatgcgct aattgggtta
    18721 gtcgtcggca tcattattgg tgctgtggcc atgcgttttg gtaatcgtaa actacgccag
    18781 caacaggcgt tgcagtacga actggaaaag aataaagctg aactggacga gtatcgcgaa
    18841 gagctggtta gccactttgc ccgcagcgcg gaattactgg ataccatggc gcacgactat
    18901 cgccagctgt atcagcacat ggcgaaaagc tccagcagcc tgctgccgga actgtctgca
    18961 gaagcaaacc cgttccgtaa tcgtctggca gagtctgaag ccagcaacga tcaggcaccg
    19021 gtgcagatgc ctcgcgacta ttctgaaggc gcatccggcc tgctgcgtac tggcgcgaag
    19081 cgcgactaat ttatttttcg ggcgcagcca ttgcgccctc ctcttctctc cctccccgac
    19141 tatcatttaa tctggtgtct cattgttagc cgtctgaaaa ttcaataaca tcaaactgtt
    19201 ttgaatctct tttcttatca ttcaggtacg agagcaggaa taatgaaaaa acaaacccag
    19261 ctgttgagtg cattagcgtt aagtgtcggg ttaactctct cggcgtcatt tcaggccgtc
    19321 gcgtcgattc caggccaggt tgccgatcag gcccctctcc ccagtctggc tccaatgctg
    19381 gaaaaagtgc ttccggcagt ggtgagcgta cgggtggaag gaacggccag tcagggacag
    19441 aaaatcccgg aagaattcaa aaagtttttt ggtgatgatt taccggatca acctgcacaa
    19501 cccttcgaag gtttaggctc cggtgtcatc atcaacgcca gtaaaggcta tgtgctgacc
    19561 aacaaccatg tgattaatca ggcacagaaa atcagtattc agctcaatga tgggcgcgag
    19621 tttgatgcaa aactgattgg tagcgatgac cagagcgata tcgccctgtt acaaattcaa
    19681 aacccgagca aattaacgca aatcgctatt gccgactccg ataaattgcg cgtcggtgat
    19741 tttgccgtag cggtcggtaa cccatttggc cttgggcaaa ccgccacctc tggcattgtt
    19801 tccgcattag gccgcagcgg gttgaatctt gaaggtctgg aaaactttat ccagacagat
    19861 gcttccatta accgcggtaa ctccggcggt gcactattaa accttaacgg tgagttaatt
    19921 ggcatcaaca ctgcaatcct tgcgcctggc ggcgggagcg tcgggattgg atttgccatc
    19981 cccagtaata tggcgcgaac actggcgcag cagcttatcg actttggtga aatcaaacgc
    20041 ggtttgttag gcatcaaagg caccgagatg agtgccgata tcgccaaagc cttcaacctt
    20101 gacgtgcagc gtggcgcgtt tgtcagcgaa gtgttgccag gttctggctc ggcaaaagcg
    20161 ggcgtcaaag cgggcgatat tattaccagc ctcaacggca aaccgctgaa tagctttgct
    20221 gagttgcgct ctcgtatcgc gaccaccgag ccgggcacga aagtgaagct tggcctgctg
    20281 cgtaacggca aaccactgga agtagaagtg acgctcgata ccagcacctc ttcgtcggcc
    20341 agcgctgaaa tgatcacgcc agcgctggaa ggtgcaacgt tgagcgatgg tcagctaaaa
    20401 gatggcggca aaggtattaa aatcgatgaa gttgtcaaag gaagcccagc tgctcaggct
    20461 ggcttgcaaa aagacgatgt gatcattggc gtcaaccgcg atcgggtgaa ctcgattgct
    20521 gaaatgcgta aagtgctggc ggcaaaaccg gccatcatcg ccctgcaaat tgtacgcggc
    20581 aatgaaagca tctatctgct gatgcgttaa tgtcgtaaac cgggcatcag gcttacgtgt
    20641 gatgtccggt taactcgtgg tatgctgctg ccgttccctt ttttaatgac gcctccatca
    20701 tgtttgtgaa gctcttacgt tccgttgcga ttggattaat tgtcggcgct attctgctgg
    20761 ttgccatgcc ttcgctgcgc agccttaacc cgctttccac tccgcaattt gacagtaccg
    20821 atgagacgcc tgccagctat aatctggcgg ttcgccgcgc cgcgccagcg gtggttaacg
    20881 tttacaaccg tggtttgaac accaactctc acaaccagct tgagatccgc accctgggat
    20941 ccggtgtaat catggatcaa cgcggttata tcatcaccaa taaacacgta atcaacgacg
    21001 ccgatcagat catcgtcgcc ttacaggatg gacgtgtatt tgaagcattg ctggtgggat
    21061 ctgactctct aaccgatctg gcggtactta aaattaatgc cactggcggt ttacctacca
    21121 ttccgatcaa tgcacgtcgc gtaccgcaca ttggcgacgt ggtactggcg atcggtaacc
    21181 cgtacaacct cgggcagacc attacccagg ggattattag tgctacgggt cgaatcggtc
    21241 tgaacccgac cgggcggcaa aacttcctgc aaaccgatgc ttccattaac cacggtaact
    21301 ctggcggcgc gctggtgaac tcgctgggag aactgatggg cattaacacg ctgtcgtttg
    21361 ataagagtaa cgatggcgaa acgccggaag gtatcggctt tgcgattcct ttccagttag
    21421 caaccaaaat tatggataag ctgatccgcg atggtcgcgt gatccgcggc tacattggta
    21481 tcggcggacg cgagatcgca ccactgcacg cgcagggcgg tggtatagat caactgcaag
    21541 ggatcgtggt taatgaagtg tcacctgacg gcccggcggc gaatgcgggt attcaggtca
    21601 acgatctgat tatttcggtg gataacaaac cggccatctc tgctctggag acgatggatc
    21661 aggtggcaga aattcgccct ggttcggtga tcccggttgt agtgatgcgt gatgataagc
    21721 agttaacgct gcaggtcacc attcaggaat atccggcaac caattaagtc gagtgctcaa
    21781 aacaaaaaac cggagtctgt gctccggttt tttattattc gctaattaat tacttattaa
    21841 cgaactcttc gcccagggcg atatctttct tcagcgtatc cagcatacct tccagtgcgc
    21901 tctgttcaaa tgcgctcagg gtaccgatag atttacgctc ttccacgccg tttttaccca
    21961 gcagcagcgg ttgagagaag aaacgtgcgt actgaccgtc gccttcaaca taggcacatt
    22021 cgacaacgcc ttgttcgccc tgcagtgcgc gtaccagaga cagaccaaaa cgtgcagctg
    22081 cctggcccat agacagggtt gcagacccgc caccggcttt cgcttcaacc acttcagtac
    22141 ctgcgttctg gatacgtttg gtcagatcag ccacttcctg ctcggtaaaa ctaacgccag
    22201 gaacctgtga cagcagcggc agaatggtaa caccagagtg accgccaata accggcactt
    22261 caacttcgcc tggctgtttg cctttcagtt ccgcaacaaa ggtgttggaa cgaatgatat
    22321 ccagcgtggt aacgccgaac agtttgtttt tgtcataaac accggctttt ttcagcactt
    22381 cagcagcaat cgcaactgtg gtgttaaccg ggttagtgat aataccaatg cacgctttcg
    22441 ggcaggtttt cgaaacttgc tgtaccaggt ttttcacgat gccggcgtta acgttaaaca
    22501 ggtcggaacg atccataccc ggtttacgcg ctacacctgc agagataaga acgacatctg
    22561 cgccttccag cgccggagtc gcatcttcac cagaaaaacc tttgattttc acagcagtag
    22621 ggatatggct cagatcgaca gccacaccgg gagtcactgg agcgatatca tacagagaga
    22681 gttctgaacc tgaaggcagt tgggttttta acagtagtgc aagcgcctgg ccaataccgc
    22741 cagcagcgcc gaggactgcg actttcatcc taaactcctt attatattga taaactaaga
    22801 tatgttgctc cgctgccgcg accttaatcc acaaaattgc cgtttacaat gaccacatct
    22861 caagaatgtg tagtcacgca agtttagcgt ttatgcattt aattgccgta atcaggaacc
    22921 taacttacgt aattaacagt cgcttatgca ttagcgcaac attccaacag gtggtgacaa
    22981 tataccctac cgttcagcca aaacaacatc aatttgataa caattaattt acttttaagc
    23041 agaatttgca tgccgtgacg caggcatgtt tctcaataac gaaatttgat aaaatcccgc
    23101 tctttcataa cattatttca gccttcttca gggctgactg tttgcataaa aattcatctg
    23161 tatgcacaat aatgttgtat caaccaccat atcgggtgac ttatgcgaag ctcggctaag
    23221 caagaagaac tagttaaagc atttaaagca ttacttaaag aagagaaatt tagctcccag
    23281 ggcgaaatcg tcgccgcgtt gcaggagcaa ggctttgaca atattaatca gtctaaagtc
    23341 tcgcggatgt tgaccaagtt tggtgctgta cgtacacgca atgccaaaat ggaaatggtt
    23401 tactgcctgc cagctgaact gggtgtacca accacctcca gtccattgaa gaatctggtg
    23461 ctggatatcg actacaacga tgcagttgtc gtgattcata ccagccctgg cgcggcgcag
    23521 ttaattgctc gcctgctgga ctcactgggc aaagcagaag gtattctggg caccatcgct
    23581 ggcgatgaca ccatctttac cacccctgct aacggtttca ccgtcaaaga cctgtacgaa
    23641 gcgattttag agctgttcga ccaggagctt taatctctgc cccgtcgttt ctgacggcgg
    23701 ggaaaatgtt gcttatccct ctcaatcccc tgctttcccc tgcgattcat ttaacgaata
    23761 gtgcgtttta ccgcgacata tcattcacac aatgaataca tacggtaaaa aaagcacatt
    23821 a
//
LOCUS       DAPHDW010000065        23803 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.065, whole genome shotgun sequence.
ACCESSION   DAPHDW010000065 DAPHDW010000000
VERSION     DAPHDW010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.065"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            190..684
                     /gene="napF"
                     /locus_tag="RH057_002506"
     CDS             190..684
                     /gene="napF"
                     /locus_tag="RH057_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HDV3128861.1"
                     /translation="MKIDASRRGILTGRWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGAGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQPQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            674..937
                     /gene="napD"
                     /locus_tag="RH057_002507"
     CDS             674..937
                     /gene="napD"
                     /locus_tag="RH057_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HDV3128862.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            934..3420
                     /gene="napA"
                     /locus_tag="RH057_002508"
     CDS             934..3420
                     /gene="napA"
                     /locus_tag="RH057_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase catalytic subunit NapA"
                     /protein_id="HDV3128863.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNRRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKYPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            3427..4122
                     /gene="napG"
                     /locus_tag="RH057_002509"
     CDS             3427..4122
                     /gene="napG"
                     /locus_tag="RH057_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HDV3128864.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            4109..4972
                     /gene="napH"
                     /locus_tag="RH057_002510"
     CDS             4109..4972
                     /gene="napH"
                     /locus_tag="RH057_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HDV3128865.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILLGSALTGMLIWEWI
                     NPVSLMGRSLVMGFGSGSLLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AATDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            4969..5418
                     /gene="napB"
                     /locus_tag="RH057_002511"
     CDS             4969..5418
                     /gene="napB"
                     /locus_tag="RH057_002511"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HDV3128866.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQERMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            5428..6030
                     /gene="napC"
                     /locus_tag="RH057_002512"
     CDS             5428..6030
                     /gene="napC"
                     /locus_tag="RH057_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HDV3128867.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSRLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            6049..6666
                     /gene="ccmA"
                     /locus_tag="RH057_002513"
     CDS             6049..6666
                     /gene="ccmA"
                     /locus_tag="RH057_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HDV3128868.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQNLLWIGHQPGIKTRLTALENLHFYHRDG
                     DTAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            6663..7325
                     /gene="ccmB"
                     /locus_tag="RH057_002514"
     CDS             6663..7325
                     /gene="ccmB"
                     /locus_tag="RH057_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HDV3128869.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            7367..8104
                     /gene="ccmC"
                     /locus_tag="RH057_002515"
     CDS             7367..8104
                     /gene="ccmC"
                     /locus_tag="RH057_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HDV3128870.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     ILVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            8101..8310
                     /gene="ccmD"
                     /locus_tag="RH057_002516"
     CDS             8101..8310
                     /gene="ccmD"
                     /locus_tag="RH057_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HDV3128871.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            8307..8786
                     /gene="ccmE"
                     /locus_tag="RH057_002517"
     CDS             8307..8786
                     /gene="ccmE"
                     /locus_tag="RH057_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HDV3128872.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            8783..10726
                     /gene="ccmF"
                     /locus_tag="RH057_002518"
     CDS             8783..10726
                     /gene="ccmF"
                     /locus_tag="RH057_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HDV3128873.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLAIAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTRDGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            10723..11280
                     /gene="dsbE"
                     /locus_tag="RH057_002519"
     CDS             10723..11280
                     /gene="dsbE"
                     /locus_tag="RH057_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HDV3128874.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            11277..12329
                     /gene="ccmH"
                     /locus_tag="RH057_002520"
     CDS             11277..12329
                     /gene="ccmH"
                     /locus_tag="RH057_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HDV3128875.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVQEGKRAGYIVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTHSSDPNDNR
                     LGGELLRQLVRSDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(12364..13011)
                     /gene="narP"
                     /locus_tag="RH057_002521"
     CDS             complement(12364..13011)
                     /gene="narP"
                     /locus_tag="RH057_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HDV3128876.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGDGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRAGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            13331..15922
                     /gene="yejO"
                     /locus_tag="RH057_002522"
                     /pseudo
     CDS             13331..15922
                     /gene="yejO"
                     /locus_tag="RH057_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
     gene            complement(16026..16102)
                     /locus_tag="RH057_002523"
     tRNA            complement(16026..16102)
                     /locus_tag="RH057_002523"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(16066..16068),aa:Pro,seq:ggg)
     gene            complement(16177..17937)
                     /gene="pbgA"
                     /locus_tag="RH057_002524"
     CDS             complement(16177..17937)
                     /gene="pbgA"
                     /locus_tag="RH057_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HDV3128877.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDEQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQINRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEDEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            complement(17957..18184)
                     /locus_tag="RH057_002525"
     CDS             complement(17957..18184)
                     /locus_tag="RH057_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HDV3128878.1"
                     /translation="MPQISRYSDEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFARALQSSINEDKAH"
     gene            18366..19373
                     /gene="yejK"
                     /locus_tag="RH057_002526"
     CDS             18366..19373
                     /gene="yejK"
                     /locus_tag="RH057_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HDV3128879.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            complement(19511..19795)
                     /gene="rplY"
                     /locus_tag="RH057_002527"
     CDS             complement(19511..19795)
                     /gene="rplY"
                     /locus_tag="RH057_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HDV3128880.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(19920..21680)
                     /locus_tag="RH057_002528"
     CDS             complement(19920..21680)
                     /locus_tag="RH057_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV3128881.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDTPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRLTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            21829..22524
                     /gene="rsuA"
                     /locus_tag="RH057_002529"
     CDS             21829..22524
                     /gene="rsuA"
                     /locus_tag="RH057_002529"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HDV3128882.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            22552..23742
                     /gene="bcr"
                     /locus_tag="RH057_002530"
     CDS             22552..23742
                     /gene="bcr"
                     /locus_tag="RH057_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HDV3128883.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVPA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALANTIDQLI
                     VMRFFHGLAASAASVVINALMRDIYPKEEFSRMMSFVMLVTTIAPLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISALLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
BASE COUNT         5353 a         5883 c         6742 g         5825 t
ORIGIN      
        1 gtgagcaaaa gcccgttttt gccacgcttc ccgccactct tttgatcctg ctacaggttt
       61 taccccgatc ggggtatgca tctttgacac atcctttaat atcttagcgg ctataaaaat
      121 ggcttattaa ttatgcggtt tatttggccg ctctcaattt tcagagcgcg ttaatgatgg
      181 aaggtcaatg tgaagattga tgcatcccgt cggggcatac tcactggtcg ctggcgcaaa
      241 gccagtaacg gtatccgtcc gccttggtcc ggtgatgaat ctcattttct gacccattgc
      301 acccgttgcg acgcctgtat taatgcttgt gaaaacaaca ttctgcaacg cggcgcgggc
      361 ggctatccga gcgttaattt caaaaataac gagtgcagct tctgttacgc ctgcgcgcag
      421 gcttgccccg aatcgctatt ttctccgcgc cacaccaggg cctgggattt gcagttcacc
      481 attggggacg cgtgcctggc gtatcagtca gttgaatgcc gccgctgtca ggatagctgt
      541 gagccaatgg caattatctt tcgcccgacg ctctccggga tctaccagcc gcaacttaat
      601 agccaacttt gcaacggatg tggagcatgt gcagccagct gtccggtatc agccataact
      661 gcggagtatc tccatgcaca ctaactggca agtttgcagc ctggtcgtgc aggccaaaag
      721 cgaacgaatt tcagacatca gcacccaact gaacgccttt cccggctgtg aagttgctgt
      781 cagcgacgcg ccgagcggtc agttgattgt ggtggtggaa gcagaagaca gcgaaacgct
      841 gatccaaacc attgagtcag tacgcaacgt agagggcgtg ctggcggtgt cgctggttta
      901 tcaccagcag gaagagcaag gtgaggaaac accatgaaac tcagtcgtcg tagctttatg
      961 aaagctaacg ccgttgcggc cgctgcggcg gctgccggtc tcagcgtgcc gggcgttgcc
     1021 cgcgccgttg ttggtcagca ggaagccatt aaatgggata aagcgccgtg ccgtttctgc
     1081 ggtactggtt gcggcgttct ggtcggaacg cagcaggggc gtgtggtggc ctgtcagggc
     1141 gacccggacg caccggttaa ccgtggcctg aactgcatta agggctattt cctgcccaag
     1201 atcatgtacg gtaaagaccg tttgacgcag ccgctgctgc gtatgaaaaa cggtaaatat
     1261 gacaaagaag gcgaatttac cccaatcacc tgggatcagg ccttcgatgt gatggaagag
     1321 aaattcaaaa ccgccctgaa agaaaaagga ccggaatcga tcggtatgtt cggttctggt
     1381 cagtggacta tctgggaagg ttatgccgcg tccaagctgt tcaaagcggg cttccgttcg
     1441 aacaacatcg acccgaacgc acgtcactgt atggcgtcgg cagtagttgg ctttatgcgt
     1501 acctttggta tggatgagcc gatgggctgc tatgacgaca tcgagcaggc tgacgcgttt
     1561 gtgctgtggg gcgctaacat ggcggagatg cacccgatcc tctggtcacg catcactaac
     1621 cgtcgtctct ctaaccagaa cgtcaccgtg gcggtgcttt ctacctacca gcatcgtagc
     1681 ttcgagctgg cggataacgg catcatcttt acgccacagt ctgacctggt gatcctgaac
     1741 tacatcgcca actatatcat tcaaaacaat gcgataaatc aggacttctt cagcaagcac
     1801 gttaacctgc gcaaaggggc gacggacatc ggctacggtt tacgtccgac ccatccgctg
     1861 gaaaaagcag cgaagaatcc gggttctgac gcctccgaac cgatgagctt tgaagattac
     1921 aaagccttcg ttgccgagta tacgctggaa aaaactgccg aaatgaccgg cgtgccgaaa
     1981 gaccagttag aacaactggc gcagctgtat gccgatccga acaagaaagt catctcctac
     2041 tggacgatgg gcttcaacca gcatactcgt ggcgtgtggg ccaacaacct ggtctacaac
     2101 ctgcacctgc tgaccggcaa aatttcccag ccgggttgcg gtccgttctc cctgaccggg
     2161 cagccttccg cgtgtggtac tgcgcgtgaa gtgggcacct ttgctcaccg tctgcctgcg
     2221 gacatggtgg tgactaacga gaaacaccgt gatatttgcg agaagaagtg gaatatcccg
     2281 agcggcacca ttccggcgaa aatcggtctg cacgcggtgg cacaagaccg tgcactgaaa
     2341 gacggcaagc tgaatgttta ctggaccatg tgtaccaaca acatgcaggc cgggccgaac
     2401 attaatgaag agcgtatgcc gggctggcgc gatccgcgca acttcatcat cgtctccgat
     2461 ccgtatccga cagtcagtgc gctggccgcc gacttgatcc tgccgaccgc aatgtgggta
     2521 gagaaagagg gcgcttacgg taacgccgaa cgccgtactc agttctggcg tcagcaggta
     2581 caggcgccag gcgaagcgaa atcggatctc tggcagttag tccagttctc ccgccgcttc
     2641 aaaactgaag aagtatggcc ggaagagctg ctggcgaaga aaccggaact gcgtggcaaa
     2701 acgctgtacg aagttctgta tgccaccccc gaagtgagca aataccctgt atccgaactg
     2761 gcggaagatc agctgaacga tgaatcccgc gagctgggct tctatctgca aaaagggctg
     2821 ttcgaagagt acgcatggtt tggtcgcggt cacggtcacg atctggcacc gttcgatgac
     2881 taccacaaag cgcgcggtct gcgctggccg gtggtgaacg gtaaagaaac gcagtggcgt
     2941 tacagcgaag gtaacgaccc gtacgtgaaa gcgggcgaag gctataagtt ctacggtaaa
     3001 ccggatggca aagcggtgat cttcgcactg ccgttcgaac cggcggcgga agcaccggat
     3061 gaagagtacg acctgtggct ctctaccgga cgcgttctgg agcactggca caccggcagt
     3121 atgactcgcc gtgtgccgga actgcaccgc gccttcccgg aagcggtctt gtttattcac
     3181 ccgctggatg cgaaagcgcg cgatctgcgc cgtggcgaca aagtgaaagt ggtttctcgc
     3241 cgtggcgaag tgatctcgat tgttgaaacg cgcggtcgta accgtccgcc acagggcctg
     3301 gtgtacatgc cgttcttcga cgccgcacag ctggttaaca aactgacgct ggatgcgacc
     3361 gatccgctct cgaaagagac ggacttcaag aagtgcgcag tcaaactgga gaaggtgtaa
     3421 gccattatgt cccggtcagc gaaacctcaa aatggtcgcc gccgctttct gcgcgatgtc
     3481 gttcgcacag caggcgggct ggctgccgtg ggtgtggcgc tggggttaca acagcaaacc
     3541 gcacgcgcat ctggcgtgcg gttgcgcccg cccggagcca taaacgagaa cgcctttgcc
     3601 agtgcctgtg tgcgttgcgg tcagtgtgtt caggcttgcc cttacgacac cttaaaactg
     3661 gcgacgctgg cctctggtct gtcggcgggc acgccatatt ttgtcgcacg ggatattcct
     3721 tgcgaaatgt gtgaggacat tccgtgcgcc aaagtgtgcc caagcggtgc gctggatcgt
     3781 gagattgaat cgatcgacga cgcgcggatg gggctggcgg tactggtgga ccaggaaaac
     3841 tgtctcaact ttcaggggct gcgctgcgat gtttgttatc gcgaatgccc gaaaattgat
     3901 gaggccatca ccctggagct ggagcgcaac acgcgtaccg gtaagcacgc ccgctttctg
     3961 ccgacggttc acagcgacgc ctgtaccggt tgcggtaagt gcgaaaaagt atgcgtgctg
     4021 gaacaaccgg caatcaaggt gttaccgctg tcactggcga aaggggagtt aggtcaccat
     4081 taccgcttcg gttggctgga ggggaacaat ggcaaatcgt aaacgtgacg ccgggcgcga
     4141 ggcgctggag aaaaaaggct ggtggcgcag tcaccgttgg ctggtgttac gtcgcctttg
     4201 tcagttcttc gtgctgggga tgtttttgag cggtccgtgg tttggtgtgt ggatcttaca
     4261 cggtaactac agcagtagcc tgttattcga caccgttccg ctgaccgatc cgctgatgac
     4321 gctgcaaagt cttgccagtg gtcatctgcc tgccacggtg gcgttgaccg gtgcggtgat
     4381 tatcaccgtg ctctacgccc tggcaggaaa gcgattattt tgcagctggg tctgcccgct
     4441 gaacccgatt accgacttag caaattggtt acgcaggcgc tttgacctca atcagtctgc
     4501 gacgatccct cgccacattc gctacgtgct gttggtggtc attctgctgg gatcggcctt
     4561 aaccggcatg ctcatctggg aatggatcaa ccccgtttcc ctgatggggc gtagcctggt
     4621 aatgggcttc ggcagcggct cgctgcttat tctcgcgctg tttttatttg atctactggt
     4681 cgttgaacac ggctggtgcg ggcacatttg tccggttggc gcgctgtatg gcgtgctggg
     4741 tagcaaaggt gtgattaccg ttgcggccac cgatcggcag aaatgtaacc gctgtatgga
     4801 ttgttttcat gtttgcccgg aaccgcatgt gctacgtgcc ccggtgctgg atgaacaaag
     4861 cccggtgcag gtcaccagcc gcgattgcat gacgtgcggt cgctgcgtgg atgtctgttc
     4921 tgaggatgta tttacaataa ctacacgatg gagttcggga gcgaaatcat gaaaagccat
     4981 gacctgaaga aagcgctgtg tcaatggacg gcgatgctgg ccctggtggt aagcggcgcg
     5041 gtttgggcgg ctaatggcgt cgattttagc cagtcgccgg aagtatccgg gacacaggaa
     5101 ggggccattc ggatgccaaa agagcaggaa cggatgccgc tgaactatgt gaatcagccg
     5161 ccaatgatcc cgcatagcgt tgaaggttat caggtaacga ccaataccaa ccgctgcttg
     5221 cagtgccacg gtgtcgaaag ttatcgcacc actggcgcgc cgcgtatcag tcctactcac
     5281 tttatggaca gcgacggcaa agtgggcgcg gaagtggctc cgcgtcgtta tttctgtctg
     5341 caatgtcacg taccgcaggc cgataccgcg ccaatcgtgg ggaatacctt taccccatca
     5401 aaaggttacg ggaaataaga ggttattatg ggaaattctg accgtaagcc tggtctgatt
     5461 aagcgcctgt ggaaatggtg gcgtaccccc agccgtctgg cgctggggac gctgctgttg
     5521 atcggttttg ttggcggcat cgtcttctgg ggtggcttta acaccgggat ggaaaaagcc
     5581 aataccgaag agttctgcat tagctgccac gaaatgcgca acacggtgta tcaggaatac
     5641 atggattccg tgcactacaa caaccgtagc ggcgtccgtg cgacctgtcc ggattgtcac
     5701 gttccgcacg agtttgtgcc gaagatgata cgcaagctca aagcaagtaa agagctgtat
     5761 ggtaaaattt ttggcgttat tgacacgccg cagaaatttg aagctcatcg tctgacgatg
     5821 gcacagaatg agtggcggcg catgaaggac aataactcgc aggagtgccg taactgtcac
     5881 aacttcgagt atatggatac aaccgcccag aaatcggttg ccgcgaagat gcatgaccag
     5941 gcggtgaaag atgggcaaac ctgtattgat tgccataaag ggatagcgca caagctgccc
     6001 gatatgcgtg aagtcgagcc aggtttttaa cagggttatt gcgtgggtat gcttgaagcc
     6061 agagagttac tttgtgagcg ggatgaacga accttattta gtggcttgtc atttacgctg
     6121 aacgcaggag agtgggtaca aatcaccggt agcaacggcg cggggaagac aacgcttctc
     6181 cgtttgctga cggggttgtc tcgccctgac gcaggcgagg ttctctggca agggcagccc
     6241 ttgcatcagg tacgcgacag ctaccatcaa aacctgttat ggataggcca tcagccgggg
     6301 atcaaaaccc ggctgacggc gttagaaaat ctgcactttt atcatcgcga tggcgatacc
     6361 gcacaatgtc tggaagccct ggcgcaggcc gggcttgccg gattcgaaga tattcctgta
     6421 aatcagctct cggccgggca acaacgccgc gtcgctttag cgcgtctgtg gctgacccgt
     6481 gccacgttat ggatcctcga cgagcctttt accgcgattg acgttaacgg cgtcgatcgt
     6541 ctgacccagc gtatggcgca gcatacggag caggggggga ttgtgattct gactacccac
     6601 cagccgctca acgttgctga aagtaaaatt cgccgcattt cactgacgca aacgagggcc
     6661 gcatgatgtt ctggcgcatt ttccgtcttg agctgcgtgt agcgtttcgc catagcgccg
     6721 aaatcgccaa cccgctgtgg ttcttcctga ttgtaattac cctttttccg ctcagtatcg
     6781 gtccggagcc gcaactgctg gcgcgtattg caccgggcat tatctgggtt gctgcgctgc
     6841 tttcatcctt gctggcgctg gaacgactgt tccgtgacga tttgcaggac ggcagtcttg
     6901 aacaattgat gttgttgccg ttacccttgc ccgccgttgt gctggcgaag gtgatggcgc
     6961 actggatggt aaccggtctg ccgttactca tcctttcgcc actggtagca atgctactgg
     7021 gaatggatgt ttatggctgg caagtgatgg cgctgacgct tctgctggga acgcctacgc
     7081 ttggctttct cggtgcaccg ggtgtggcgt tgacagtggg acttaagcgc ggtggtgtgc
     7141 tgctcagcat actggtgtta ccgctgacta tcccattact catctttgcc accgccgcga
     7201 tggacgcggc ttccatgcat ttgcccgttg acgggtatct ggcaatttta ggcgcgttgc
     7261 tggcaggcac cgcgacatta agtccttttg cgacggcggc agcgttacga atcagcattc
     7321 aataacgctg gattactgat ttttgttcga gtctggtatc gaaactatgt ggaaaacact
     7381 gcatcaactg gcgatccctc cacggctgta tcaaatctgt ggctggttta taccgtggct
     7441 ggcaattgcc agtgtggtcg tgcttaccgt cggctggatc tggggattcg gctttgctcc
     7501 ggctgattat cagcagggaa atagctaccg cattatctac ctgcatgtgc ctgcggcgat
     7561 ctggtcgatg ggcatttatg catcaatggc ggtggcggct tttattggcc ttgtctggca
     7621 gatgaaaatg gccaacctgg cggtggcggc gatggccccc attggtgccg tgtttacctt
     7681 tattgccctg gttaccggct ctgcatgggg aaaaccgatg tggggcacct ggtgggtatg
     7741 ggatgcacgt ctgacttctg aactggtgct gctgtttttg tatgtgggtg tgattgccct
     7801 gtggcacgcc ttcgacgacc gccgtctggc gggccgtgcg gcaggtatcc tggtgctgat
     7861 tggcgtggtg aatctgccga ttattcatta ctccgtggag tggtggaaca ccctgcatca
     7921 gggatcaacg cggatgcagc aaagtatcga tccggcgatg cgttcgccgc tgcgctggtc
     7981 gatttttggc ttcctgctgc tgtctgccac gctgacgctg atgcggatgc gtaatttgat
     8041 attgctgatg gaaaaacgcc gtccgtgggt gagtgaactg atactgaaaa gaggccgtaa
     8101 atgacccctg catttgcttc ctggaatgaa tttttcgcaa tgggcggtta cgcctttttt
     8161 gtctggctgg cggtggtgat gaccgttatt ccgctggtgg ttttggtcgt gcactcggtg
     8221 atgcagcatc gcgcaattct gcgtggtgtg gcgcaacagc gggcgcgtga ggcgcgttta
     8281 cgtgctgcgc aacagcagga ggctgcatga atattcgccg taaaaaccgc ttgtggattg
     8341 cctgtgccgt gttggcaggg ctggcgctga ctatcggtct ggtgctatat gcgctgcgct
     8401 cgaatatcga tctcttttat acgccggggg aaattctcta cggcaagcgt gaaactcagc
     8461 aaatgccgga agtcgggcag cgtctgcgcg tcggcgggat ggtgatgccg ggtagcgtgc
     8521 agcgcgatcc caattcgcta aaagtaactt tcaccattta cgacgctgaa ggttcagtag
     8581 atgtctctta cgaaggcatt ttgccggatc tgttccgtga agggcagggc gttgtggtgc
     8641 agggcgaact ggaaaaaggc aatcatatcc tcgcaaaaga agtgctggcg aaacacgacg
     8701 aaaactacac gccgccagaa gttgagaaag cgatggaagc caatcaccgt cgcccggcga
     8761 gtgtttataa ggacccagca tcatgatgcc ggaaattggt aacgggctgc tgtgtctggc
     8821 gctaggaatt gcgctgctgc tgtccgtgta tccgctatgg ggcgtagcgc gcggagatgc
     8881 gcgcatgatg gcgtcttccc gcttgtttgc ctggctgctg tttatgtctg tggctggcgc
     8941 atttctggta ctggtcaatg ccttcgtggt caacgacttc accgtcacct atgttgccag
     9001 caactccaat acccagcttc cggtgtggta tcgcgtggcg gctacctggg gcgcgcatga
     9061 aggctcgctc ctgctgtggg tgctgctgat gagcggctgg acctttgcgg tagcgatttt
     9121 tagtcagcgt attccgctgg atattgtggc ccgcgtactg gcgataatgg ggatggtcag
     9181 cgtcggcttt ttgctgttca ttctctttac ctctaacccg ttctcacgca cgttgccgaa
     9241 cttcccgatt gaagggcgcg atcttaaccc gctgttacag gatccggggc tgatcttcca
     9301 tccgcctctg ctctatatgg ggtacgtggg gttctcggtg gcgtttgctt ttgccattgc
     9361 ttctttgttg agcgggcgtc tggacagcac ttatgcgcgt tttactcgtc cgtggacgct
     9421 ggcggcgtgg atcttcttga cgctcggcat tgtgctcggt tccgcatggg cctattacga
     9481 actcggctgg ggcggctggt ggttctggga tccggtagaa aacgcctcgt ttatgccgtg
     9541 gctggtgggg actgcgctga tgcactcact ggcggtcact gaacaacgcg ccagcttcaa
     9601 agcgtggaca ttactgctgg caatcagtgc cttctcgttg tgtctgctgg ggactttcct
     9661 ggtgcgttcc ggcgtgctgg tatcggtaca cgcgtttgcc tctgatccgg cacgcggtat
     9721 gtttatcctc gcctttatgg tactggtgat tggtggttcg ctgctgctgt ttgccgcgcg
     9781 tggacacaaa gttcgctcac gcgtaaacaa tgcgctgtgg tcgcgggaat ctctgctgtt
     9841 agcgaacaac gttttgctgg tcgccgcgat gctggtggta ttactgggga cgctgctgcc
     9901 gctggtgcac aagcaactgg gactgggcag tatttcgatt ggcgaaccgt tcttcaacac
     9961 catgtttacc tggctgatgg tgccgtttgc gctgctgctt ggtgtcggtc ctctggtgcg
    10021 ctgggggcgc gatcgcccac gtaaaatccg caatttattg attatcgcct tcatctcaac
    10081 gctggtgctg tcgctgctgc tgccgtggct gttcgaaagc aaagttgtgg cgatgacggt
    10141 gctcggcctg gcaatggcct gctggattgc tgtgctggca attgcggaag ctgcgctgcg
    10201 tatttcacgt ggcacgaaaa ccaccttcag ttattgggga atggtggcgg ctcacctggg
    10261 gctggcagtg acaattgttg gtattgcctt tagccagaac tatagcgttg agcgtgatgt
    10321 gcgcatgaag tccggcgata gcgtcgatat tcatgaatat cgcttcacct tccgtgatgt
    10381 caaagaggtg actggcccga actggcgtgg cggtgtggcg actatcggcg taacgcgcga
    10441 tggcaagccg gaaacggtgc tgtatgcgga aaaacgttat tacaacactg ccgggtcgat
    10501 gatgaccgaa gcggcaattg acggcggcat cacgcgtgac ctgtacgccg cgctcggtga
    10561 agagctggaa aacggcgcgt gggccgtgcg tctttactac aaaccatttg ttcgctggat
    10621 ttgggcgggc gggctgatga tggcgttggg cggactactg tgtctgtttg atcctcgcta
    10681 tcgtaagcgc gtgagtccgc aaaaaactgc gccggaggcc gtatgaagcg caaagtattg
    10741 ttaattccgt tgattatctt cctggcgatt gccgcggcgc tgctgtggca gctggcgcgt
    10801 aatgccgaag gggatgatcc gaccaatctg gaatcggcgc tcattggcaa gcctgtgccg
    10861 aagtttcgtc tcgaatcact ggacaatccg gggcagtttt atcaggcgga tgtgctgact
    10921 cagggcaaac cagtgctgct taacgtctgg gcgacctggt gtccgacctg ccgtgcggaa
    10981 catcaatatc tgaatcagct ttcagcgcag ggcattcgcg tggtcggcat gaactataaa
    11041 gacgatcgcc agaaggcaat cagctggctg aaagagctgg gcaatcctta cgcgctaagc
    11101 ctgtttgatg gcgacggcat gttagggctg gatctcggtg tctatggcgc gccagaaacc
    11161 ttccttattg acggcaacgg tatcattcgc tatcgccatg cgggcgatct caatcctcgc
    11221 gtctgggaag aagagatcaa gccgctgtgg gagaaataca gtaaggaggc cgcacaatga
    11281 ggtttttatt gggcgtgctg atgctgatga tctccggctc agcactggcg accatcgacg
    11341 tgttgcagtt taaagatgaa gcacaggaac aacagttccg tcagctcact gaagaactgc
    11401 gctgcccgaa atgccagaac aacagcattg ccgattccaa ctcgatgatt gccaccgacc
    11461 tgcgtcagaa agtgtatgaa ctgatgcagg aaggtaaaag taaaaaagag attgtcgatt
    11521 atatggtggc gcgttacggc aacttcgtca cttacgatcc gccgttaacg ccgctgaccg
    11581 tgctgctgtg ggtgcttccg gtagtggcta ttggcattgg cggttgggtc atatacgccc
    11641 gctcgcggcg tcgggtacgc gtagtgccgg aagcgtttcc tgaacaaagc gtgcaggaag
    11701 gtaagcgtgc cggatatatt gtttatttgc cgggcattgt ggtggcgtta attgtggctg
    11761 gcgtcagcta ctaccagacc ggaaattatc agcaggtgaa aatctggcag caggccacgg
    11821 cacaggctcc ggcgttgctg gacagggcgc tggatccgaa agccgatccg ctcaacgaag
    11881 aagagatgtc gcgtcttgcg ctggggatgc gtactcaact gcaaaaaaat ccgggagata
    11941 tagaaggctg gattatgttg ggccgcgttg gcatggcgct gggtaacgct agtatcgcca
    12001 ccgatgcata cgctactgcg tatcgcctcg atccgaaaaa cagcgatgcc gcactgggtt
    12061 atgctgaagc gttgacacat tcatctgatc ccaacgacaa ccgcctcggt ggtgaactgc
    12121 tgcgccagtt ggtgagaagc gaccacagca atatccgtgt gttaagcatg tatgcgttta
    12181 atgcctttga gcagcagcga tttggcgaag ccgttgccgc gtgggagatg atgttgaaac
    12241 tcttacctgc caacgatact cgccgtgcgg tgattgaacg tagtatcgcg caggcgatgc
    12301 aacatttgtc gccgcaggag agtaaataaa aagaggcccg atggtgatgc catcgggcta
    12361 tttttattgt gccccgcgtt gttgcaggaa cagaatggtg gctgccacgc gtgagcggac
    12421 attgagtttg cgcagcagat tgcgaatatg tacttttact gtctgctctg aaatattcaa
    12481 caccgaggca atctgtttat ttgacagccc ctgtgccagc tcgtgcagaa catccagctc
    12541 gcgctccgtc agcacgctga agggatcttc ttccgcgcca aacatttcac gttcacgtaa
    12601 gtactgattg acgcgttcgc taaagacttt gctgcctttc gctccagcac gaattgcttc
    12661 cagcaatact tccgggtcgc tgtctttcaa cagataaccg tctgcgcctg cgtctatcag
    12721 cgcaaagaca tcgctggagg catctgatac ggtcaggata ataatttgcg cggtaacgcc
    12781 atccctgcgc aaggcattga gggtatccag gccactcata cctttcatat tgagatccag
    12841 caagatcacg tcgatatcca gtctattcgc cagatcgatc gcgctcgcgc cgtcgcccgc
    12901 ttcggcgacc acttcaaagc caggatcaag ctccagtaac tgacgaacac cgcgtcgcat
    12961 aagtggatga tcatccacaa tcatcacctg aaaaggcgtt gcttcaggca tagtagtctc
    13021 ctgaggtttt attagaatca ttatgtttga gcgtagcgtt ggtaacgcta ccaggcgcgt
    13081 gaatgttacc ccattttatg tatggacatg tagtgaaaac tacaaccttg tcgttcgctt
    13141 tggcatcatt ttcattataa agacggaatt ttgcatcagc ttctcatcaa gaaaaacaac
    13201 taaaaaatca atcacaatac ttgtgcattt ctttaaacag tttttaaaaa ctgactccgg
    13261 gtatggagct atgggtattt tccgtaccca atgcttttaa cagcaattaa tttcatagga
    13321 tgaaagctca attcatcaat ctggttctgt ttctctttgt cgttccgcaa tatctgttct
    13381 ggtggctaca gcgttatatt cacccatagc attggcatca acagttgagt atggtgagac
    13441 agttgatggt gttgtcctgg aaaaagatat ccagctggtt tatgggaccg ccaataatac
    13501 gaaaatcaat cctggcggag aacagcatat aaaagaattt ggtgtaagta gtaatactga
    13561 aattaaagga gggtatcagt acattgaaat gaatggtacc gcagaatact cagtattaaa
    13621 tgacggttat caaattgttc aaatgggtgg cgcggcaaac cagactaccc tcaataatgg
    13681 tgtgctacag gtttatggcg cagcgaatga tcccacgatt aaaggcgggc gcttaatcgt
    13741 tgaaaaagat gggattaccg tccttgccgc tatcgaaaag ggaggattac tggaggttaa
    13801 agagggggga ttagcgattg cggtagatca gaaagcaggc ggtgctatta aagcaagcac
    13861 gcgggtcatg gaggcattcg gaacaaaccg tctcggtcag ttcgaaatca agaatggtat
    13921 tgctaacaat atgctgttgg aaaacggcgg aagtttgcga gttgaagaaa atgacttcgc
    13981 ttataatact actgtagata gtggcggctt actggaggtt atggatggcg ggactgtaac
    14041 tggcgttgat aaaaaagcag gcggaaaatt aattgtctca acgaatgcgc tggaagtgag
    14101 tggtacaaac agtaaaggcc aatttagtat aaaagatggt gtgtcaaaaa attatgaact
    14161 ggatgatggt tccgggctca ttgttatgga ggacacgcag gccattgata ctatccttga
    14221 tgagcatgcc actatgcaat cgctgggaaa ggatactggt acgaaagtgc aggcaaatgc
    14281 ggtatatgat ctcggtcgat cagatcagaa tggaagtatc acgtattcct caaaagccat
    14341 ctctgaaaat atggttatca acaatggccg cgctaacgtc tgggctggca caatggttaa
    14401 cgtttcagtc agagggaatg atggcattct tgaggtcatg aagccgcaaa taaattatgc
    14461 acccgcaatg ttggtgggta aggtagtggt ttctgagggc gcttctttta gaacgcatgg
    14521 tgccgtggat accagcaaag cggacgtttc gctcgaaaat agtgtatgga ccatcattgc
    14581 cgatatcact acgacgaacc aaaacaccct cctcaactta gccaaccttg cgatgtctga
    14641 cgcaaatgtg attatgatgg atgagccagt gactcgttca tcagtgacgg caagtgcgga
    14701 aaatttcatt acgttgacca ccaataccct gtcgggaaac ggcaattttt atatgcgtac
    14761 cgatatggct aatcatcaga gcgatcagct caacgccacc ggtcaggcaa caggtgattt
    14821 caaaatattc gtgacggaca ccggtgccag cccggcagca ggagatagcc ttacactggt
    14881 aacaacgggc ggcggtgatg ctgcatttac gttgggcaat gccggaggcg ttgttgatat
    14941 cggtacgtat gaatatacct tgctggataa tggcaaccat agctggagtt tggcagagaa
    15001 tcgcgcgcaa attacccctt caaccactga tgtgctgaat atggcggcag cacaaccgct
    15061 ggtatttgat gcagaactgg acaccgtgcg tgagcgtctt ggtagcgtaa aaggcgttaa
    15121 ttacgatacg gcgatgtgga gttcggcaat taacacccgc aacaacgtga ccactgatgc
    15181 gggcgctggc tttgagcaaa cattgaccgg gctgacgctc ggtatcgata gccgtttctc
    15241 ccgtgaagaa accagcacaa ttcgcggctt gttctttggt tactctcatt ctgatattgg
    15301 ttttgatcgc ggcggcaaag gcaatgtcga tagctatacc ctgggggctt atgccggttg
    15361 ggagcatcag aacggtgcct atgttgatgg agtggtgaaa gttgaccgtt ttgccaacac
    15421 catccattgc aagatgagta atggggcaac agcgtttggc gattacaata gtaacggcgc
    15481 gggtgctcat gtcgagagcg ggttccgttg ggttgacgga ttgtggagtg ttagacccta
    15541 tctggccttt accggcttta ccacagatgg tcaggactac acgttatcaa acggcatgcg
    15601 cgcggatgtg ggaaataccc ggatattacg cgctgaagcg ggaacggcgg taagctatca
    15661 catggacctg caaaacggta cgacgctgga accctgactg aaagccgccg tgcgtcagga
    15721 atacgccgat tctaaccagg tgaaagttaa tgacgatggc aaatttaata atgatgtggc
    15781 tggaacccgt ggcgtttatc aggctgggat aaggtcatcg tttaccccga cgttaagcgg
    15841 tcatttgtca gtcagctatg gcaatggcgc aggggtagaa tcgccgtgga atactcaggc
    15901 gggtgtggtc tggacgttct gataacaaaa aataaacagg ctgtgatgtg tcacggtctg
    15961 tttatcgaat taattgcaga tataaaaaaa accaaccgta aaggttggtt ttttcttggg
    16021 atttttggtc ggcacgagag gatttgaacc tccgaccccc gacaccccat gacggtgcgc
    16081 taccaggctg cgctacgtgc cgactcgtgg ctgctaatac taccgttttc cacaccgatt
    16141 gcaagtaaga tattttgcta actgatttat aattaatcag ttagcgataa aacgcttctc
    16201 gtctgtcagc acttgcaata acaaacttaa ctgtggtttt tcatctttca ctctttcacc
    16261 acgtaagttg taagtgcggt atttaccgtt attgttcagc accagcgtct ttttcggggt
    16321 ggtaattgcc agcgtatcgt tatccgctgc agtaacccag taatgacggc gttgagggtt
    16381 gaacaaatcc tgaccttgcg aatattcgct ggcaggtgtg ctgacatgta gcaggcgttg
    16441 catcagcgtc gtcatcagat cggtatgatc agtcagagca ttaatacgct gcgccggcgt
    16501 gcctggccag tgaatcacta atggcacctg cagatgaccg tgggaccagt caaaggtttc
    16561 ttcgtcttcg ctcagcggaa taccccgacc ggcagtgata atcaccaccg tattgtccag
    16621 tttgccagaa tcacgcagtg cattgagcac gcggttgatc tggtcatcga cattgcctgc
    16681 cgcccggcta tatttccgtg caaatgcctg ctgattgctg tcgtcaatgt tagtgccatt
    16741 gaaagagacc cacgagaacc agcggttatc ttcttgtgcg tagcggccca gccagttgat
    16801 ccactgcgtg gcggtctgct cgtcggattg ggtgcgtacg ctcggcatcg agaaatctga
    16861 caacaatgcc tggcgataca gcgggctggt aaagccatct gatgagaata atcccagctg
    16921 atagccttgc tgattaagcg cagtaattaa cgccgcaggg gtacgggtcg acagaatgcc
    16981 gtccatatag ctcggcgaga tgccatagaa caggccaaag atgccgttgt ctgtagtgtt
    17041 gccggagctc atatggcgcg tgaacgaaat attttgctca gcaaaacctg ccagcgcagg
    17101 catctgcttc tcgaagcgtg agtagttcag gccatcgaca gtaatcaaca gcacattctg
    17161 cccggtgccc atatcgcgat agcgcagttc gcttaacgga tactgaacgg aaacggcgtc
    17221 tggattacct tgctcaataa gacggcgttg atactcctgc gcatcaagca gaccatgctt
    17281 ctcaagaaaa cgtcgcgccg tcatcgggta cgaaagcggc aggttagcgc gctgcatggt
    17341 gatcgggcga tagaagttgg catcggccca gatatacacc acatgcgagg cgataaaggc
    17401 gataaataag aatgcggcca gcgggcgcgc gaagcgccga cgacgcgtca ggctgcgcag
    17461 cttttgccag ctccacgtcg caaacaccag ttcaagcaat aaaataaccg gcacgctgat
    17521 gaacatcagc tgccagtcgc gcgccatctc attttcgtct gggttgataa ccagttgcca
    17581 gacgatggga ttaagatgga gatggaaacg agtaaagact tcgctatcga tcagtaatag
    17641 cgtcattccc gccgttgcca gaatgacgga caaaaacctc atcagcctct gggagccgac
    17701 gataaaggtc agcgggaaga ggatcagcaa gtaggtggcg aacaccagga agctgaaatg
    17761 gccgataatg cttacgtagg aataaatgcg accagcaagc gttgtcggcc agtcggcgat
    17821 aaacaggtaa cggctgccaa tgacgagcga aagcagaata ttgaacagtg caaaccagtg
    17881 cccccaactg accatctggg agactttttc acggtagcgc tgacgatgag ttaccataac
    17941 ctgttatctg tttcccttag tgcgctttgt cttcgttgat agaggactgt aaggcgcggg
    18001 caaaagagtt ggcaattgcc tggcgttggg ccggggcaat gctggtattg ataaggttag
    18061 tgaccatgtt gcctaacacc atcagggaaa ggtcggtcgg agccttatgt ttttccagta
    18121 cgttgagcag ctctgcgagc aattgttcaa cctgttcatc actgtagcgg gaaatttgtg
    18181 gcataaatcg aaatcagtta gttgatgaaa gggcaacata ttaccgtagc aacggttttt
    18241 tttctgcttt tttatgcgtt aaatcattct cgttacgctg ccttctgtaa aatgaccgcc
    18301 atcttttatt gcgtcgctta accggcggtg gttgaatacc gcccggtctt aaaggagagt
    18361 ttatcatgag tctggatatc aaccagattg ctctgcacca gcttatcaag cgtgatgagc
    18421 agaaccttga gctggtgttg cgcgattcat tgctggaacc gacagaaacc gtcgttgaga
    18481 tggtggcaga actgcatcgg gtctatagcg ccaaaaataa agcttacggc ttgtttagcg
    18541 aagagagtga actggcgcag acgctgcgct tacagcgtca gggcgaagag gactttctgg
    18601 catttagccg cgcggcaact ggtcgcctgc gtgacgaatt agcgaaatat ccttttgctg
    18661 acggtggatt cgtgctgttt tgccactacc gttatctggc ggtggagtat ctgctggtgg
    18721 cagttctgag caacctgagc agtatgcgcg ttaacgaaaa tctcgacatc aatccaaccc
    18781 attatcttga tatcaatcat gcggatattg ttgcgcgtat cgatttaacc gaatgggaaa
    18841 ccaatccaga gtccacccgc tatctcactt tcctgaaagg gcgagtaggg cgcaaagtgg
    18901 cggatttctt tatggatttc ctcggggcca gcgaagggtt aaacgcgaaa gcgcaaaacc
    18961 gtgggctgtt gcaagcggtt gatgatttca ctgccgaagc acaactggat aaagcagagc
    19021 gacagaacgt gcgccagcag gtttatagct actgtaatga gcagctgcag gcaggcgaag
    19081 agatcgaact ggagtcgctg tcgaaagagc tggcaggcgt cagcgaagtg agtttcacgg
    19141 agtttgcggc tgaaaaaggc tacgaactgg aagagagctt cccggcagac cgtagcacac
    19201 tgcgtcagtt gacgaagttt gccggtagcg gcggtggctt gactattaac tttgatgcga
    19261 tgctgttagg tgaacgcatc ttctgggatc cggcaaccga taccctgacc attaaaggca
    19321 cgccgccgaa tttgcgtgac caattgcagc gccggacgtc tggcggcaat taatcggttt
    19381 tgtgttctga atgccagatg cggcgtgaac gccttatctg gcctgcaaaa aatagcaata
    19441 ccgtaatcag gcataatctg cagatacaaa aaccccgccg gagcggggtt ttttacaact
    19501 tattcagcaa ttaagcgcga acgaagtcga tgtgctgcag cttcggtttg tacgggtgac
    19561 gctgtacgtc ctgagcttta actttgattt ctttaccgtc aacaacgatg gtcagaactt
    19621 cgctgtagaa ttcagcttta gcttgcatgt tcatgacttt gtcgtgatcc agctcgatag
    19681 ccagcggcgc ttctttgcca ccgtagatga ttgccgggaa cttgttagcg gcacgcaggc
    19741 ggcggctcgc acccttaccc tgctctttac gtacttctgc gttgatagta aacatttctt
    19801 tctctcttaa attaatcctg ctacaggcga cccagcaaca ggtaagtgat ctgctttgcg
    19861 tatgcaaaag cgggcgagat tctatactca atcgctttat acatcaatca aaactaccat
    19921 taaccgcgta attcgtgcgc ccgacgaaaa cgaccttcgt aatcgaacac tttttcacgc
    19981 acttgccagt actgaccttt catgcgggca acgacaaaat ccgggtgtcg caataaggct
    20041 tgctgggcga ggatatcggc ggcggtgatc cagcgcagag ggatgcccgg tgtgcgcgta
    20101 tgcgggcgga taaaaagctg ctcgaaggca gttcgctggg cgggcgtttg cagacggaaa
    20161 cgctcactca catccgcgcc gtcttcatcg taataggtga ttttcaacca ttcgcctttc
    20221 tcgtcgtgcc catgttgcaa agacatgccg ctacagcgta ataccagcgc gtctttcagt
    20281 cgtagcgccg cttttaacat atcgtccgga tcaaccagta cggtgtcaca ttcgcggcag
    20341 cggcgggcgg caatatcatt ttctgcgtta cattgcgggc aatttttaaa acggaaacgg
    20401 aagtcacact gttcgcgatg accgtcgtca tcttcaaacc atccctgaca gcgacgacca
    20461 aagtgttcaa tcaatgtccc gtcggcggtc gttttccccc aaaaggtgtt ggcaaaaccg
    20521 caggcagggc agaaaacctg taccgggacg ttgtcacttt tgcctttcgg tgtaccaact
    20581 tccggcgcgt agagatcgtg aggattaccc gcataatcaa gaattaagca atcagtcttg
    20641 ccaggagcga gacgcagacc gcgcccgaca atttgttggt aaagactcac tgattcggta
    20701 aggcgcagaa tggcgataag atcgacgtgc ggggcgtcaa atccggtggt cagtactgcg
    20761 acgttgacca gatagcgaaa acgctgggct ttaaaatctt caattaacac atcgcgctca
    20821 gcgccggggg tgtcgccagt aatcagtgca gcatcttcgg cgggcagtaa tcccacaatc
    20881 tcttttgcgt gttcgaccgt cgcggcgaaa atcattaccc ctttgcgctt ttcggcgaac
    20941 tccatgatct ggctgatgat atgtggcgta atgcgttgct gttttttcag ctcacggttg
    21001 agatcagctt cgctgaacag cccgttactc tgtgcctgca agcggctgaa atcgtattgt
    21061 actactggca tatccagtcg ttctggcggc gtcagatagc cgtgtttaat catataacgc
    21121 agcggcagct cataaatgca gtcacggaaa agggctttct catcgccgcg taccatgccg
    21181 tgataatgga actggtagat ccagcctttg cccaatcgaa aaggcgtggc agtcagcccc
    21241 agcaggcgta agtggggatt cacttttgtc aggtgagtga ggatttgctg atactggctc
    21301 tcttcatcgt caccaatacg gtgacattca tcgacaatca acagcgaaaa ttcaccctga
    21361 aaggcatcaa gattacgggc gaccgactgc acactgccaa ataccacttt accgtggctc
    21421 tctttacgct ttagcccggc ggcaaaaata tcggcttcca gccccagcgc ctgatacttt
    21481 gcatggtttt gcgccaccag ttctttaacg tgtgccagca ccagtacgcg accacgagcc
    21541 agccgtgcca gttccgctat caccaggctt ttacctgcgc cggtgggcag cacgataacg
    21601 gcaggggttt tatgacgacg aaaatggttg agcgtggcat ccacggcttc ttgctgatat
    21661 gggcgaagtg taaaaatcat ggtctcgcgt aggtagatta ttctgctaat agtatgccac
    21721 ggttcttttt cccttgaggg aagttattag accgctatac tgggcagaca acagttcaga
    21781 ttttcgggtc aatgcccgac ctccgttttt acaggcaaaa tcacacacat gcgacttgat
    21841 aaatttatcg cacagcaact cggcgttagc cgtgctattg ccgggcgtga aatccgcggc
    21901 aatcgtgtca ccgtcgatgg cgaaatcgtc cgtaatgcag cgttcaaact gcttcctgaa
    21961 catgatgtcg cttacgatgg caacccgctg gcgcagcaac atggtccacg ttacttcatg
    22021 ctcaataagc ctcagggcta tgtttgctcc acggacgacc ctgatcaccc aacggtgctc
    22081 tattttctgg atgaaccggt agcctggaaa ctgcacgccg caggacgtct ggatatcgat
    22141 accaccggtc tggtgctgat gactgatgat ggtcagtggt cgcaccgcat tacttctccg
    22201 cgccatcatt gcgagaagac ctatctggtg acactggaat cacctgtagc tgacgatacg
    22261 gcagagcaat ttgccaaagg cgtacagctg cataacgaaa aagatctcac taagcctgcg
    22321 gtgctggaag tgattacccc aacgcaggtt cgtctgacca tcagcgaagg gcgttatcat
    22381 caggtgaaac gcatgttcgc cgctgtgggt aaccatgtgg ttgagctgca tcgtgaacgt
    22441 attggcggta ttacgctgga tgctgattta gcccccggtg aatatcgtcc gttaactgaa
    22501 gaagaaattg ccagcgtcgt ctaacttttc catgactttc aggagcccgt tgtgaccacc
    22561 cgacagcatt cgtcgtttgc tattgttttt atccttggcc tgctggccat gttgatgccg
    22621 ctgtcgattg atatgtatct gcccgcgcta ccggtaattt cagcgcagtt tggcgtacct
    22681 gcgggcagta cgcagatgac cctcagtact tatattctgg gctttgcgtt ggggcagtta
    22741 atctacgggc cgatggcaga cagcttcggg cgtaagccgg tggtgctcgg cggtacgctg
    22801 gtgtttgccg ccgccgcggt ggcgtgtgcg ttggcaaaca ccatcgatca gctgattgtg
    22861 atgcgtttct tccacgggct ggctgcgtct gcggccagcg tggtcattaa cgccctgatg
    22921 cgcgatattt acccgaaaga agagttctcg cggatgatgt cgtttgtcat gctggtgacc
    22981 actattgcac cgctgatggc accgattgtc ggcggttggg tgctggtgtg gctgagctgg
    23041 cattacatct tctggatcct ggcattagcg gcgattctgg cttcggcaat gattttcttc
    23101 ctgattaaag aaaccttacc accggagcgt cgccagcctt ttcacattcg taccactatt
    23161 ggtaactttg cggcgctgtt ccgtcataag cgtgtcctga gttatatgct tgccagtggt
    23221 ttcagctttg ccgggatgtt ctcgttctta agcgccggac cgtttgtcta cattgaaatt
    23281 aaccacgtcg cgccggaaaa ctttggttat tactttgcac taaacattgt tttcctgttc
    23341 gtgatgacca tttttaacag ccgcttcgtc cgccgcattg gcgcgttaaa tatgttccgc
    23401 tcggggttgt ggatacagtt tattatggcg gcgtggatgg tcatcagtgc gctgctgggg
    23461 ctgggattct ggtcgctggt ggttggcgtt gcggcgtttg tgggctgcgt gtcgatggtg
    23521 tcatccaatg cgatggcggt cattcttgat gagtttcccc atatggcggg aacggcatct
    23581 tcgctggcag gaaccttccg ttttggcata ggggcaattg ttggcgcatt gctttctctt
    23641 gcgaccttta actctgcatg gccgatgatt tggtcaattg cattctgcgc aaccagctcc
    23701 attctcttct gtctgtacgc cagtcggccg aaaaaacggt gatctattgc acaacgagga
    23761 agctaaaagg cttcctttgt tgatgcatgt caaccacaaa tct
//
LOCUS       DAPHDW010000066        23572 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.066, whole genome shotgun sequence.
ACCESSION   DAPHDW010000066 DAPHDW010000000
VERSION     DAPHDW010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.066"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            8..397
                     /locus_tag="RH057_002531"
     CDS             8..397
                     /locus_tag="RH057_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128884.1"
                     /translation="MKRYIKWFAITIFISMLSACARTAPVQQISTTVSVGHTQEQVKN
                     AILKAGAQRKWIMTQVSPGVIKARYQTRNHVAEVRITYTATYYNIKYDSSLNLQASDG
                     KIHKNYNRWVRNLDKDIQVNLSTGATL"
     gene            462..1520
                     /locus_tag="RH057_002532"
     CDS             462..1520
                     /locus_tag="RH057_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312352.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV3128885.1"
                     /translation="MYEQDTLSALDAITEAQRIAFAPMLFQAALCLRNAGILDYLDQQ
                     GKQGAPLNAITEHTVLNEYAVGVLLDMGLSGRIITCKEGIYYLAKIGHYLLHDTMTRV
                     NMDFTQDVCYQGLFFLADSLNEGKPSGLKVFGDWPTIYPALSQLPDAARDSWFAFDHY
                     YSDGAFNAALPYVFANNPTTLYDVGGNTGKWALRCCKFNENIAVTLLDLPQQIVLAKE
                     NIANAGFSDRIDFHAVDMLSDAPLPGEADIWWMSQFLDCFSPEQIISILSKIASVMKP
                     GAKLCIMELFWDAQRFEATSFSLNASSLYFTCMANGNSRFYSAEKFYDYLNKAGFQVA
                     ERHDNLGVGHTLLICQKK"
     gene            1561..2283
                     /locus_tag="RH057_002533"
     CDS             1561..2283
                     /locus_tag="RH057_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312353.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl synthase chain length factor"
                     /protein_id="HDV3128886.1"
                     /translation="MKFALNITNWQALAPGLSDVQQWQAWSRQPWAIDPAAPLAKLSE
                     LPMMTARRLSSGSKLAVECGLTMLRRHQPDAVLYTSRHGELERNYRIVHALATEQALS
                     PTDFALSVHNSSVGNLTIVAKQPIVSSSLSAGRDSFQQGLCEVLSLLQAGYQRVLMVD
                     FDGFLPEFYHPQLPAEMPTWPYAVALVIEAGDDWQCETQPAIAVNETTLPQSMLFLQH
                     YLQNADAFSLPGERVQWRWSRR"
     gene            2280..3101
                     /locus_tag="RH057_002534"
     CDS             2280..3101
                     /locus_tag="RH057_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312354.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HDV3128887.1"
                     /translation="MSKLMSRLEWTWRLVMTGLCFVLFGLGGLLLSVVWFNILLVLVW
                     DTSRRRRLARRSIAASFRLFLTVAKGLGVLDYRIDGAEILRQERGCLVVANHPTLIDY
                     VLLASVMPETDCLVKSALLKNPFLGGVVRAADYLINSEAETLLPRCQQRLAQGDTILI
                     FPEGTRTKPGEKMTLQRGAANIAVRCTSDLRIVTIGCSEHLLDKQSKWYDVPPARPFF
                     TVEVHGRVEINKFYDATSQEPTLAARQLNRHLLLQLQQGCLPLSGINNASALSGN"
     gene            3076..3333
                     /locus_tag="RH057_002535"
     CDS             3076..3333
                     /locus_tag="RH057_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3128888.1"
                     /translation="MQALYLEIKNLIISTLNLDDLTPDDIDINAPLFGDGLGLDSIDA
                     LELGLAVKNEYGIVLSAESEEMRQHFFSVATLASFIAAQRA"
     gene            3345..3596
                     /locus_tag="RH057_002536"
     CDS             3345..3596
                     /locus_tag="RH057_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3128889.1"
                     /translation="MTDQQTIYQEVSALLVKLFEIDPQDIKPEARLYEDLELDSIDAV
                     DMIVHLQKKTGKKIKPEEFKAVRTVQDVVEAVERLLQEG"
     gene            3601..4182
                     /locus_tag="RH057_002537"
     CDS             3601..4182
                     /locus_tag="RH057_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128890.1"
                     /translation="MSGIRSLPMIKLLTGLLLLAWPFLIWLGLVHNSLHWLLPLMALL
                     LLLRLRQPRRQTGPLQVVTKIVAVVGIVLCVSSFLLKTHQLLLFYPVVVNAVMLAVFG
                     GSLRSTMPIVERLARLQEPDLPEKAVRYTRRVTQVWCLFFIFNGSIALFTTLYGNMPL
                     WTAWNGIIAYLLIGFLTAGEWLIRCQMIKRKTP"
     gene            4179..5537
                     /locus_tag="RH057_002538"
     CDS             4179..5537
                     /locus_tag="RH057_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HDV3128891.1"
                     /translation="MNQTLSLSQWLTASRPVTTPVAWLGEYTWTLGHLRHDVALLIDH
                     LRDQPGNRWALCFENSYLFIVALLATLHSGKIPVIPGHNRAALLNVQRSLFDCVLSDK
                     RLGWNGPQFVVSTSHQMTTSTIAFDDIASDAFIELFTSGSTGQPKQIAKPVISLDQEA
                     NMLATHFADRLLGCRFVASVMPQHLYGLTFRIFLPMALGLPLHAAMLWYSEQLAALSH
                     TYRYAFVSSPAFLKRLDLHLTPPPVEMILSAGGMLPWQDVEKTSTWLNIWPDEIYGST
                     ETGILAWRYRQQDNIPWFTFSDVHLSQESEGVRVFSPLIPAEGLVLDDMLQFNENGQF
                     HLIGRRGRVVKIEEKRISLVEIEQRLLALDGIMDVAAIPLIRNGRQAIGVVVVPNKEA
                     RQIWQCSGGKTLELSWRKALLPWLEPVAVPRYWRIIDEIPVNNMNKRVYAQLQELFHD
                     TP"
     gene            5524..5877
                     /locus_tag="RH057_002539"
     CDS             5524..5877
                     /locus_tag="RH057_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009008217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymyristoyl-ACP dehydratase"
                     /protein_id="HDV3128892.1"
                     /translation="MIRHEIERHQVQPQEMEIVLYLDPMLYWFNGHFAVQPLLPGVAQ
                     LDWVMHYATTLLAPGWRFRSIQNVKFLAPLIPETTVTLQLTWQETSQVLTFCYQRHDG
                     DARHTASSGKIRLCR"
     gene            5868..7544
                     /locus_tag="RH057_002540"
     CDS             5868..7544
                     /locus_tag="RH057_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV3128893.1"
                     /translation="MSVNFSPCVLIPCYNHGAMMPGVLARLKPFNLPCIVVDDGSDAA
                     TQQQLDNLVAEQPGVTLIRLAENTGKGAAVMRGLQAAADAGFSHAVQVDADGQHAIED
                     IPKLLALAEQHPVALISGQPIYDDSIPRSRLYGRWVTHVWVWIETLSLQLKDSMCGFR
                     VYPVAPTLQLAKYATIGKRMDFDTEVMVRLYWQGNTSYFVPTRVTYPQDGLSHFDALK
                     DNVRISLMHTRLFFGMLPRIPSLLMRRSSSHWARQSEVKGLWGMRLMLLVWRLLGRTA
                     FSALLYPVVGVYWLTAARARKASQDWLARVRQHQPQAAKLNSYQHFLRFGNAMLDKIA
                     SWRGELQLGHDVLFAPGAEAALDVRDPRGKLLLASHLGDVEVCRALAKIQGYKTINAL
                     VFSENAQRFKQIMQEMAPQAGINLMPVTDIGPETAILLKEKLDNGEWVAIVGDRIAVT
                     PQRGGDWRVCWSPFMGQPAPFPQGPFILASILRCPVNLIFALRQRGKLHIHCETFADP
                     LLLPRGERQQALQNAIDHYAARLEHYALQSPLDWFNFFDFWQLPEIQDKE"
     gene            7548..7970
                     /locus_tag="RH057_002541"
     CDS             7548..7970
                     /locus_tag="RH057_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HDV3128894.1"
                     /translation="MLNDPRFTAEVELTIPFHDVDMMGVAWHGNYFRYFEVAREALLN
                     QFNYGYRQMKESGYLWPVVDARVKYRHALTFEQRIRVRAHIEEFENRLRIGYQIFDAE
                     TGKRATTGYTIQVAVEEQSRELCFVSPDILFERMGVKP"
     gene            7967..8572
                     /locus_tag="RH057_002542"
     CDS             7967..8572
                     /locus_tag="RH057_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein carrier protein LolA"
                     /protein_id="HDV3128895.1"
                     /translation="MKFLPLLVLLISPFVSALTLDDLQQRFTEQPVIRAHFDQTRTIK
                     DLPQPLRSQGQMLIARDQGLLWDQTSPFPMQLLLDDKRMVQVINGQPPQIITAENNPQ
                     MFQFNHLLRALFQADRKVLEQNFRVEFADKGEGRWTLRLTPTTTPLDKIFNTIDLAGQ
                     TYLESIQLNDKQGDHTDIALTQHQLTPAQLTDDERQRFATQ"
     gene            8541..10859
                     /locus_tag="RH057_002543"
     CDS             8541..10859
                     /locus_tag="RH057_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HDV3128896.1"
                     /translation="MTNANALPPSKRPALLWGLVCLVMAAALLILLPQSRLNSSVLAM
                     LPKQAMGDIPPALNDGFMQRLDRQLVWLVSPGKEANPRVAQEWLTLLQKSAALGDIKG
                     PMDAASQQAWGAFFWQHRNGLIDPDTRARLQNGGEAQAKWILSQLYSAFSGVSGKELQ
                     NDPLMLMRGSQLAMAKNGQRLRLMDGWLVTQDPQGNYWYLLHGELAGSSFDMQQTHQL
                     ITTLNTLEKALKTRYPQAQLLSRGTVFYSDYASQQAKQDISTLGVATLLGVILLIVAV
                     FRSLRPLLLCVISIGIGALAGTAATLLIFGELHLMTLVMSMSVIGISADYTLYYLTER
                     MVHGNDVSPWQSLAKVRNALLLALLTTVAAYLIMMLAPFPGIRQMAIFAAVGLSASCL
                     TVLFWHPWLCRGLPVRPVPAMALMLRWLAAWRRNKKLSRGLPVALALFSLAGMSMLRV
                     DDDISQLQALPQHILAQEKAITALTGQSVDQKWFVVYGDSPQQTLRRLEKYTASLEYA
                     KKEGLISNYRTIPLNSLARQEEDLDLLKTAAPTVTKALQNAGLTAVKPDLNAMPVKVD
                     EWLASPASEGWRLLWLTLENGESGVLVPVEGVKSSALLQEIATYYPCGIAWVDRKSTF
                     DELFALYRYVLTGLLLVALAVIACGAVARLGWRKGLISLVPSVLSLGCGLAVLAMSGQ
                     AVNLFSLLALVLVLGIGINYTLFFSNPRGTPLTSLLAIALAMLTTLLTLGMLVFSATQ
                     AISSFGIVLVSGIFTAFLLSPLAMPDKKRTKK"
     gene            10856..11440
                     /locus_tag="RH057_002544"
     CDS             10856..11440
                     /locus_tag="RH057_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3261 domain-containing protein"
                     /protein_id="HDV3128897.1"
                     /translation="MIKSTFWRAFALTATLILTGCSHSQPEQEGRPQAWLQPGTRITL
                     PAPGISPAVNSQQLLTGSFNGKTQSLLVMLNADDQKITLAGLSSVGIRLFLVTYDAQG
                     LRAEQSIVVPQLPPASQVLADVMLSHWPISAWQPQLPAGWTLRDNGDKRELRNASGKL
                     VTEITYLNRQGKRVPISIEQHVFKYHITIQYLGD"
     gene            11442..12611
                     /locus_tag="RH057_002545"
     CDS             11442..12611
                     /locus_tag="RH057_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HDV3128898.1"
                     /translation="MIYISAVGMINALGNNLDEIAANLARGVAPGMRPRAGWLQGHPQ
                     AVLAGVDGELPLIPEKFAAHRSRNNQILLAALAQLQPQVDDAIAKYGCERIAIVLGTS
                     TSGLHEGDTHVNLHTHGQPSTTWHYAQQELGDPSRFLSHWLALDGPAYTLSTACSSSA
                     RAIISGRRLIEAGLVDAAIVGGADTLSRMPINGFHSLESLSPTLCQPFGRDRAGITIG
                     EGAGLMLLTREPQPIALLGVGESSDAYHISAPHPQGEGAIRAINQALTDAQLTPDDVG
                     YINLHGTATQLNDQIESMVVNALFGERVPCSSTKHLTGHTLGAAGITEAAISMLILQR
                     DLPLPAQDFSLSPRDPTLPPCGIIEKPQPLARPVILSNSFAFGGNNASILLGRVS"
     gene            12608..13072
                     /locus_tag="RH057_002546"
     CDS             12608..13072
                     /locus_tag="RH057_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-fatty acyl-ACP dehydratase"
                     /protein_id="HDV3128899.1"
                     /translation="MSHYLSPGAYLPHDAPMLLLEDVVSVSDDSAVCRVTVSPSGVLA
                     PFLDPDGNLPGWFALELMAQTVGVWSGWHRHQQGKNSIELGMILGARELLCAAGILPA
                     GQTLTITVKLLMQDDRFGSFECSINDGEATGRINTFQPTAEELTTLFQQGAS"
     gene            13072..13803
                     /gene="fabG"
                     /locus_tag="RH057_002547"
     CDS             13072..13803
                     /gene="fabG"
                     /locus_tag="RH057_002547"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HDV3128900.1"
                     /translation="MSRSVLVTGASKGIGRAIACQLAADGFNIGVHYHRDATGAQETL
                     NAIVANGGNGRLLSFDVANREQCREVLEHEIAQHGAWYGVVSNAGIARDAAFPALSDD
                     DWDAVIHTNLDSFYNVIQPCIMPMIGSRQGGRIITLSSVSGVMGNRGQVNYSAAKAGI
                     IGATKALAIELAKRKITVNCIAPGLIDTGMIEMEESALKEAMSMIPMKRMGQAEEVAG
                     LASYLMSDIAGYVTRQVISINGGML"
     gene            13800..15029
                     /locus_tag="RH057_002548"
     CDS             13800..15029
                     /locus_tag="RH057_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase"
                     /protein_id="HDV3128901.1"
                     /translation="MTRRVVITGMGGVTAFGENWQDVSARLLAYENAVRKMPEWQVYD
                     GLHTLLGAPVDDFTLPEHYTRKRIRAMGRVSQMSTRASELALEQAGLIGDPILTSGET
                     GIAYGSSTGSTGPVSEFATMLTEKHTNNITGTTYVQMMPHTTAVNTGLFFGLRGRVIP
                     TSSACTSGSQAIGYAWEAIRHGYQTVMVAGGAEELCPSEAAVFDTLFATSQHNDAPKT
                     TPSPFDENRDGLVIGEGAGTLILEELEHAKARGATIYGEIVGFATNCDAAHITQPQRE
                     TMQYCMEQSLKIAGLSAQDIGYISAHGTATDRGDMAESLATATIYGDNVPLSSLKSYF
                     GHTLGACGALEAWMSLQMMREGWFAPTLNLNKPDPNCGALDYIMHEARKVDCEFLQSN
                     NFAFGGINTSIIIKRWP"
     gene            complement(15869..16900)
                     /locus_tag="RH057_002549"
     CDS             complement(15869..16900)
                     /locus_tag="RH057_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3128902.1"
                     /translation="MEKTRKRRGTGRVTLQEVANFAGVGTMTVSRALRTPEQVSDKLR
                     EKIEQAVEELGYIPNRTAGALASGHSHTVAVLVPSLTDKASSRFMQSLQQVLNKNEFQ
                     LLLGCHEYNQLKEAEILMTLLQGNPAALVIFGSQLADKTHQILEKTNIPTINVIGSPF
                     SPAQITIETAFFEASHKLTEHLLEQGYKNIGFIGAHMDNRLQRQQLNGWHKAMLSHYQ
                     NTDLVITMPDAASLQLGRYALNEILLRQPELDAVICSHEEIALGIMFECQRRLLKIPG
                     NIAVACLDGSDNCDQTHPTLTSIRIDYKKMGIETGKLLIGLLNNNHDESEESRIVQFN
                     YQIELRQST"
     gene            17171..17614
                     /locus_tag="RH057_002550"
     CDS             17171..17614
                     /locus_tag="RH057_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001497294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3128903.1"
                     /translation="MLKNLLNTEVVQVVEQVKDWREAVAISCRPLIENGSIEPRYVDA
                     IYHSHDTIGPYYVVGPGIAMPHARPEEGANKLSLALTLIPSGVNFDADENDPVKLLIV
                     LAATDSTSHIEAISQLAKLFDNEKDIQAILTAKTTQDILSVIARY"
     gene            17630..17917
                     /locus_tag="RH057_002551"
     CDS             17630..17917
                     /locus_tag="RH057_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004717867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HDV3128904.1"
                     /translation="MKITVVCGNGLGTSLMMEMSIKSILKDLSVSADVDHVDLGSAKG
                     TPSDIYVGTKDIAEQLAAQSVAGKIVALENMIDKKAMRERLSVVLTELGAL"
     gene            17930..19186
                     /locus_tag="RH057_002552"
     CDS             17930..19186
                     /locus_tag="RH057_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011815933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HDV3128905.1"
                     /translation="MDFFRFLMSDVLSEPAVLVGLIALIGLIAQKKPVTECIKGTVKT
                     IMGFVILGAGAGLVVSSLGDFANIFQHAFGIQGVVPNNEAIVSVAQKSFGKEMAMIMF
                     FAMVINIMIARFTPWKFIFLTGHHTLFMSMMVAVILATAGMTGITLIAVGSLVVGVAM
                     VFFPAIAHPYMKKVTGSDDVAIGHFSTLSYVLAGFIGSKFGNKEHSTEDMNVPKSLLF
                     LRDTPVAISFTMSIIFLVTCLFAGADAVKELSGGKNWFMFSIMQSITFAAGVYIILQG
                     VRMVIAEIVPAFKGISDKLVPNARPALDCPVVFPYAPNAVLVGFLSSFAAGLIGMFTL
                     YLLNMIVIIPGVVPHFFVGAAAGVFGNATGGRRGAILGAFAQGLLITFLPVFLLPVLG
                     DIGFANTTFSDADFGALGILLGIIVR"
     gene            complement(19402..19686)
                     /locus_tag="RH057_002553"
     CDS             complement(19402..19686)
                     /locus_tag="RH057_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3128906.1"
                     /translation="MEQKTLSVEPRRSFSNEFKLQMVKLALQPRGSVARIAREHDIND
                     NLLFKWLRLSLIKGRISRRLPVTNSSGIGVELLPVEMTLDERPYPVFYAR"
     gene            complement(20108..21121)
                     /locus_tag="RH057_002554"
     CDS             complement(20108..21121)
                     /locus_tag="RH057_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HDV3128907.1"
                     /translation="MSTRINLWRALFGEKPRILLENSDFTVTSFRYDSGVEGLKIANS
                     RGHLIILPWMGQMIWDAQFDGHGLTMCNMFRQPKPATEVIETYGCFAFHSGLLANGCP
                     SAEDTHLLHGEMACAAMDEAWLELDGDMLRLNGRYEYVMGFGHHYLAQPTVVLHKSST
                     LFDIKMAVTNLASVDMPLQYMCHMNYAYIPNATFSQNIPDEILRLRESVPSHVNPTAQ
                     WLAFNQRIMQGEASLSTLSQPEFYDPEIVFFADKLDAYTEQPEFRMISPDGTTFVTRF
                     YSAELNYVTRWILYNGEQQVAAFALPATCRPEGYLAAQRNGTLIQVAPQQTRTFTVTT
                     GIE"
     gene            complement(21133..22449)
                     /gene="fucP"
                     /locus_tag="RH057_002555"
     CDS             complement(21133..22449)
                     /gene="fucP"
                     /locus_tag="RH057_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HDV3128908.1"
                     /translation="MNDKNIIQMPDGYLNKTPLFQFILLSCLFPLWGCAAALNDILIT
                     QFKSVFSLSNFASALVQSAFYGGYFLIAIPASLVIKKTSYKVAILIGLTLYIGGCTLF
                     FPASHMATYTMFLAAIFAIAIGLSFLETAANTYSSMIGPKAYATLRLNISQTFYPIGA
                     ASGILLGKYLVFSEGESLEKQMSGMNAEQIHNFKVLMLENTLEPYKYMIMILVVVMVL
                     FLLTRFPTCKVAQTSHHKRPSAMDTLRYLARNPRFRRGIVAQFLYVGMQVAVWSFTIR
                     LALELGDINERDASNFMVYSFACFFIGKFIANILMTRFNPEKVLILYSVIGALFLAYV
                     ALAPSFSAVYVAVLVSVLFGPCWATIYAGTLDTVDNEHTEMAGAVIVMAIVGAAVVPA
                     IQGYIADMFHSLQLSFLVSMLCFVYVGVYFWRESKVRTALAEVTAS"
     gene            complement(22477..23397)
                     /gene="rbsK"
                     /locus_tag="RH057_002556"
     CDS             complement(22477..23397)
                     /gene="rbsK"
                     /locus_tag="RH057_002556"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV3128909.1"
                     /translation="MDIAVIGSNMVDLITYTNQMPKEGETLEAPAFKIGCGGKGANQA
                     VAAAKLNSKVLMLTKVGDDIFADNTIRNLESWGINTTYVEKVPCTSSGVAPIFVNANS
                     SNSILIIKGANKFLSPEDIDRAAEDLKKCKLIVLQLEVQLETVYHAIEFGKKNGIEVL
                     LNPAPALRELDMSYACKCDFFIPNETELEILTGMSVDTYDHIRLAARSLVDKGLNNII
                     VTMSEKGALWMTRDQEVHVPAFKVNAVDTSGAGDAFIGCFSHYYVQSGDVEAALKKAA
                     LFAAFSVTGKGTQSSYPSIEQFNEFLTLNE"
BASE COUNT         5719 a         6009 c         5993 g         5851 t
ORIGIN      
        1 ggcactaatg aaacgttata taaaatggtt tgccatcaca atttttatca gtatgttgag
       61 tgcctgtgcc cgtacggccc cagtgcaaca gataagcacc actgtcagtg tgggtcatac
      121 tcaggagcag gttaaaaatg ccattttgaa agcaggtgcg cagcgcaagt ggattatgac
      181 gcaagtgtcc cctggagtta ttaaagctcg ctatcaaaca cgaaatcacg ttgcagaggt
      241 tcgtattaca tatacagcta cctactataa catcaaatat gacagtagcc tgaatctgca
      301 ggcttctgat ggaaaaattc ataaaaacta taaccgctgg gtgcgtaacc tagataaaga
      361 tatacaggtt aacttatcta caggagcaac gttatagtga tgctgatatc gggctggaat
      421 ggcccgatag tatttaactt tctctataag aatggttata tatgtacgaa caggacacgc
      481 tcagcgcact ggatgccatc accgaagcac agcggattgc ctttgctcct atgcttttcc
      541 aggcagcttt gtgcctgcgc aatgcaggaa tattagatta tttggatcaa cagggtaaac
      601 agggagctcc ccttaatgcg attactgagc acacagtcct gaatgaatat gccgtgggtg
      661 tgttactgga tatgggctta agcgggcgaa ttattacctg taaagaggga atttattacc
      721 tggcgaaaat cggtcattac ctcctgcatg acactatgac tcgcgtgaat atggatttca
      781 cccaggatgt ctgttaccag gggctcttct tccttgccga ttcactcaat gaaggaaaac
      841 cttccgggtt aaaagtattt ggcgactggc cgacgatata tccggcactt tcgcaattac
      901 cagacgccgc acgtgatagc tggtttgcgt ttgatcatta ctattcagat ggcgcattta
      961 acgccgcact gccttatgta ttcgccaaca accccacaac cttgtacgat gtgggtggca
     1021 atacgggtaa gtgggctttg cgctgctgca aattcaatga aaacatcgcc gttacgttat
     1081 tagatctgcc acaacaaatc gtactggcga aagaaaatat cgcgaatgca ggtttttctg
     1141 atcgcataga tttccatgcg gtggatatgc taagcgatgc cccattacca ggcgaggctg
     1201 atatctggtg gatgagccaa tttttagact gtttttcacc cgaacaaata attagcatac
     1261 tcagcaaaat tgccagcgta atgaagcctg gcgcaaaact ttgcatcatg gagttgttct
     1321 gggatgcaca gcggtttgaa gcgacatcgt ttagcctgaa tgcatcctca ctttatttta
     1381 cctgcatggc aaacggtaac agccgtttct atagtgcaga gaaattttac gactatctga
     1441 ataaagcagg attccaggta gcagagcgtc acgataattt aggcgttggg catacacttt
     1501 tgatatgcca aaagaaataa tcgtcactta ataattttgg tttaccgcgg gcgcgcgttg
     1561 atgaaatttg cattaaatat taccaactgg caggcactgg cgcctggact tagcgatgtg
     1621 cagcaatggc aagcatggtc gcgccagccg tgggcgattg accctgccgc gccgttggca
     1681 aaattaagtg aactgccaat gatgaccgct cgtcgtctca gttcaggcag caaactggct
     1741 gttgaatgcg gtctgacaat gctacgccgc catcagccag acgctgtttt gtataccagt
     1801 cgtcatggcg aactggagcg caattaccgc attgttcatg cactggcaac cgaacaggcg
     1861 ctttctccga cggacttcgc gctttccgta cacaactctt ccgtgggtaa cctgaccatt
     1921 gtggccaaac agccgatcgt ttcgtcatcg ctttcggctg gtcgcgacag tttccagcaa
     1981 ggtttgtgtg aagtgcttag cctgctacaa gcgggatacc agcgcgtgtt gatggtcgat
     2041 tttgatggct ttctacctga gttttatcac ccgcaactgc ccgcagaaat gcctacctgg
     2101 ccgtacgccg tcgcgctggt gattgaagct ggcgatgact ggcaatgtga aacgcagcca
     2161 gccattgcgg taaatgaaac aacactgccg caaagcatgt tgtttttaca gcactattta
     2221 caaaatgcag atgctttttc actgcctggc gagcgcgtgc aatggcgctg gagccgcaga
     2281 tgagtaagct gatgtcacgt ctggagtgga cctggcgact ggtcatgacc gggttatgtt
     2341 ttgtcctgtt tggtctcggc ggcctgctgc tttcagtcgt gtggtttaac atattgttag
     2401 ttttggtgtg ggatacttcc cgccgccgtc gtttggcacg tcgcagcatt gccgccagtt
     2461 ttcgcctgtt tttaactgtc gcgaaagggc ttggcgtgct ggattatcgt attgatggcg
     2521 ctgaaatttt acgtcaggaa cgcggttgcc tcgtcgtagc caatcatccc acgctgatcg
     2581 attacgtgct gctggcatca gtcatgccag aaactgactg cctggtgaaa agcgcgctgt
     2641 taaaaaatcc ttttcttggc ggcgtcgtgc gggcggcaga ttatttaatc aacagcgaag
     2701 ctgaaaccct gttaccccgc tgccaacagc gactggcaca gggtgacact atcctgattt
     2761 tccccgaagg cacacgaacg aagcccggtg aaaaaatgac gttacagcgc ggtgcagcga
     2821 atattgccgt gcgctgcacc agcgatttac gcatcgtgac gatcggctgt agcgagcatc
     2881 tgctggataa acaaagtaag tggtatgacg tcccgcctgc caggccgttt tttacggttg
     2941 aagtccatgg gcgggtagaa atcaataaat tttacgatgc aacttcacaa gagccgacgc
     3001 tggctgcaag acagttaaac cggcatcttt tgcttcaatt acagcaaggt tgtttacctc
     3061 tatcaggaat aaataatgca agcgctttat ctggaaatta aaaacctcat catcagcacc
     3121 ctcaatctgg acgacttgac tcctgatgat attgatatca atgcgccact gtttggcgac
     3181 ggtttagggc tggactctat cgatgcactg gaactgggac tggcagtaaa aaatgagtac
     3241 ggcatcgtgc tctctgcgga aagcgaagaa atgcgtcagc actttttctc cgtcgccact
     3301 ctggcctctt tcattgctgc gcaacgtgcc tgaggaaaac catcatgaca gaccaacaaa
     3361 ccatttatca ggaagtctca gccctgctgg ttaagctgtt tgaaatcgat ccgcaggaca
     3421 tcaaacctga agcgcgactg tacgaagatc tggaactgga cagcatcgac gccgttgaca
     3481 tgattgtgca cctgcaaaag aaaaccggta agaaaatcaa gccggaagag tttaaagcag
     3541 tgcgtacagt ccaggatgtt gtcgaggctg tagaacgcct gctacaagaa ggctaatttg
     3601 atgtcgggca ttcgctcgct accgatgatt aagttgctga ccggattatt gctgctcgcc
     3661 tggccttttt taatctggct aggtttggtg cataacagcc tgcactggct gttgccgctg
     3721 atggcgctgc tgcttttatt gcgcctgcgc cagccccgtc gccagacggg gccgctccag
     3781 gtcgtcacaa aaatcgttgc tgttgtgggc attgtattgt gtgtctccag ttttctgttg
     3841 aaaacgcacc agttactgct gttttacccg gtagtggtta acgcagtcat gctggcggtt
     3901 tttggcggtt cgctgcggtc gacaatgccg atagttgagc ggctggcacg tttgcaggaa
     3961 ccagacctcc cggaaaaggc cgtgcgatat acccgccgcg taacgcaggt ctggtgcctg
     4021 ttttttattt tcaacggcag tattgcgtta tttaccacct tgtatggcaa catgcctttg
     4081 tggacagcct ggaacggtat cattgcttac cttctgatag gtttcctgac tgcaggggaa
     4141 tggctgatac gttgtcagat gatcaaacgg aaaacaccat gaatcagact ctctcacttt
     4201 cacagtggtt gacagcctct cgccctgtaa caactcctgt tgcctggctt ggggaatata
     4261 cctggacact ggggcatctg cggcatgatg tagcgctttt gatcgaccat ttacgcgacc
     4321 agccaggcaa tcgctgggca ctttgttttg aaaacagcta tctttttatt gtagcgttgt
     4381 tggccacgct acactcaggt aaaatcccgg taatcccggg ccataatcgc gccgctttgt
     4441 taaatgtaca acgctccttg tttgattgcg tcctgagtga taaaaggctg ggctggaacg
     4501 gtccacagtt tgttgtaagc acatctcacc agatgacgac cagcacgata gcctttgatg
     4561 atattgcttc tgacgcgttt attgaacttt tcacatcggg ttcaaccgga caaccaaaac
     4621 aaattgcaaa acctgttatc agccttgatc aagaagcaaa catgctggca actcactttg
     4681 cagaccgact actgggctgt cgttttgtag catccgtgat gcctcagcac ctgtatgggc
     4741 tgactttccg tattttttta cctatggcac ttggcctgcc attgcatgcc gcaatgctct
     4801 ggtattctga gcaacttgcc gcattaagcc atacctaccg ctatgccttt gtcagcagcc
     4861 cagcgttcct taaacgactc gaccttcatc tgacgcctcc ccccgtggaa atgattctct
     4921 ctgccggcgg tatgctacca tggcaggatg tagagaaaac ttccacctgg ctgaatatct
     4981 ggcccgatga aatctatggt agtaccgaaa caggtattct cgcatggcgt taccgtcaac
     5041 aggataatat cccctggttt actttttctg atgttcatct ttcacaggaa agtgaggggg
     5101 ttcgtgtctt ttcgccactc atccctgctg aaggattggt gcttgacgat atgttgcaat
     5161 tcaatgaaaa cggtcagttc cacctgatcg gacgacgtgg tcgagtggta aaaatagagg
     5221 agaagcggat atcactggtt gaaatcgaac aacggcttct ggcacttgac ggtattatgg
     5281 atgtcgctgc aataccatta atacgcaatg gccgccaggc tatcggtgtc gtggttgtac
     5341 caaataaaga ggcccgtcaa atatggcaat gctccggagg gaaaactctg gagttgtcct
     5401 ggcgcaaagc actattacca tggcttgaac ctgttgcagt acctcgttac tggcgcatta
     5461 ttgatgaaat accagtaaac aatatgaata agcgtgtcta tgcacaatta caggagttat
     5521 ttcatgatac gccatgaaat tgagcgccat caggttcaac cgcaggagat ggaaatcgtt
     5581 ctataccttg acccgatgct ctactggttt aatggtcatt ttgccgtaca gcctctgtta
     5641 ccaggcgtgg cccagttgga ctgggtaatg cattatgcta ccacgctgct tgctccgggc
     5701 tggcgttttc gtagtatcca gaatgttaaa tttctggcgc cacttatacc tgagacgact
     5761 gttaccctgc aattaacatg gcaggaaaca agtcaggtac tcactttctg ctatcagcgt
     5821 cacgacggtg acgcccgcca caccgccagt agcgggaaga tccgcctatg tcggtaaact
     5881 tttctccctg cgtgttgatc ccctgctaca accacggcgc gatgatgccg ggcgtgctgg
     5941 cgcgtcttaa gccatttaat cttccctgta ttgtggtgga tgacggcagc gatgccgcca
     6001 cgcaacagca actggataat ctggttgccg aacagcctgg cgtgacctta attcgcctgg
     6061 cagaaaacac aggcaaaggc gcggcggtaa tgcgcggctt acaggcggcg gcagacgcgg
     6121 gcttcagcca tgcggtgcag gtggatgctg acggtcaaca tgcgattgaa gatatcccca
     6181 aactgctggc actcgctgaa caacaccctg tggcgctgat ctccggccag ccaatttacg
     6241 atgactccat cccccgctca cggctttacg ggcgctgggt cacccacgtc tgggtatgga
     6301 tcgaaacgct ctccctgcaa ctgaaagaca gtatgtgcgg ctttcgcgtt tatccggttg
     6361 cgccaacgct gcaactggca aaatacgcca ccatcggcaa gcggatggat ttcgacactg
     6421 aagtgatggt gcgcctctac tggcagggaa acaccagtta tttcgtgcca acccgcgtca
     6481 cttatccgca ggacgggctt tcgcattttg atgccctgaa agataacgtc cgcatctcgc
     6541 tcatgcacac gcgtctgttt ttcggcatgt tgccgcgtat tccttcacta ctgatgcgcc
     6601 gctcttccag ccactgggcg cggcagagtg aagtgaaagg attatgggga atgcgcctga
     6661 tgctgctggt ctggcgtctg ctgggaagaa cggcgtttag cgcgctgctt tacccggtgg
     6721 tgggcgtcta ctggctcact gctgctcgtg cgcgcaaagc gtcgcaagac tggctcgccc
     6781 gtgtacgaca gcatcaacca caggcggcaa aactcaacag ctatcagcac tttctacgtt
     6841 tcggtaatgc catgctcgac aaaatcgcca gctggcgcgg cgagctacaa ctggggcacg
     6901 atgtgctgtt tgcgccaggc gcagaagcgg cgcttgacgt ccgcgatccg cgcggcaaat
     6961 tgctgctggc ctcgcatctt ggcgatgtgg aagtgtgccg ggcgctggca aaaattcagg
     7021 gctacaaaac cattaacgcg ctggtgttta gcgaaaacgc ccaacgcttt aaacagataa
     7081 tgcaggagat ggctcctcag gcaggcatta acctgatgcc ggtaacagat atcggcccgg
     7141 aaaccgccat cctgctgaaa gagaagctgg ataacggcga atgggtggcg attgtcggtg
     7201 accgcatcgc cgtcaccccg caacgcggcg gcgactggcg cgtctgctgg agtccgttta
     7261 tgggccagcc tgcgcctttc ccacaggggc cgtttattct cgcgtctatt ttgcgctgcc
     7321 cggtgaatct gattttcgcc ctgcgccagc gcggcaagct gcatattcac tgcgaaacct
     7381 ttgccgaccc gctgctgttg ccgcgtggcg aacgccaaca ggcgctgcaa aacgctatcg
     7441 accactacgc cgcgcgtctg gaacattacg cgctccagtc gcccctcgac tggtttaatt
     7501 ttttcgattt ctggcaactg ccggaaattc aggacaagga gtaaagggtg cttaacgatc
     7561 cccgatttac cgctgaagtc gagctgacca ttccctttca cgacgtcgat atgatgggcg
     7621 tggcctggca cggtaactat tttcgctact ttgaagtggc ccgcgaggcg ctgctcaacc
     7681 agttcaatta cggctatcgg cagatgaaag agtcaggtta tttgtggcca gtggtcgatg
     7741 cgcgggtgaa atatcgccac gccctcacct ttgagcaacg catccgcgtg cgtgcgcata
     7801 tcgaagagtt cgaaaaccgt ctgcggattg gctatcagat tttcgatgcc gaaacgggca
     7861 aacgcgccac caccggatac accattcagg tcgcagtaga ggagcaaagc cgtgaactgt
     7921 gctttgtcag ccccgatatt ctgtttgaac gcatgggagt caaaccatga aatttttacc
     7981 gctgctggtg ctgctgatta gcccgtttgt gagcgccctg accctggacg atcttcagca
     8041 acgctttacc gaacaaccgg tgatccgcgc ccatttcgat caaacccgga cgattaaaga
     8101 tctgccgcag ccgctgcgat ctcagggtca gatgttgatc gcccgcgacc aggggttatt
     8161 gtgggatcaa acctcaccgt tccccatgca actattactg gacgataaac gcatggtgca
     8221 ggtgatcaac ggtcagccgc cgcaaatcat cacggcagaa aacaacccgc agatgttcca
     8281 gtttaaccac ctgctgcgcg cgctgttcca ggccgatcgc aaagtgctgg aacaaaactt
     8341 ccgcgtcgaa tttgccgaca aaggcgaagg ccgctggacg ctgcgcctga cgccgaccac
     8401 cacgccgctg gacaaaattt tcaacaccat cgatctcgcc gggcaaacct atctggagag
     8461 cattcagctt aatgataaac agggcgatca caccgatatc gctcttaccc aacatcaact
     8521 gacaccagcg caactgaccg atgacgaacg ccaacgcttt gccacccagt aaacgccccg
     8581 cgctgttatg ggggctagtc tgcctggtca tggcggcggc gttgctgatc ctgctgccac
     8641 aatcacgact gaacagtagc gtgctggcta tgttacccaa acaggcgatg ggcgatattc
     8701 ctccggcgct gaatgacggc tttatgcagc gtctggaccg ccaactggtg tggctggtca
     8761 gccccggtaa agaggctaat cccagggtgg ctcaggagtg gctgacgctg ctgcaaaaat
     8821 ccgctgcgct cggcgacatt aaagggccaa tggatgccgc cagccagcag gcgtggggag
     8881 cgtttttctg gcagcaccgc aacggcctga ttgaccccga cacccgcgcc cgcctgcaaa
     8941 atggcggcga agcgcaggcg aagtggatcc tctcccagct ttattctgcg ttctccgggg
     9001 taagcggcaa ggagctgcaa aacgatccgc tgatgttaat gcgcggctcg caactggcga
     9061 tggcgaaaaa tggccagcgt ttacgactaa tggacggctg gctggtgacg caggatcccc
     9121 agggcaatta ctggtatctg ctgcacggcg aactggcggg atcgtcgttt gatatgcagc
     9181 aaacccacca gctcatcacg accctgaata cgctggaaaa ggctctgaaa acgcgttacc
     9241 cgcaggcgca gttgctctca cgcggcacgg tgttttacag cgattacgcc agccaacagg
     9301 cgaagcagga tatctccacc ctgggcgtgg caacgctgct gggggtgata ttgctgattg
     9361 tggcggtgtt ccgctctttg cgcccgttgc tgctttgcgt gatttccatc ggcatcggcg
     9421 cgctggcggg aacggccgcc acattgttga ttttcggtga attacacctg atgacgctgg
     9481 tgatgagcat gagcgttatc ggcatttccg ctgactacac gctctattat ctcaccgagc
     9541 ggatggtgca cggcaacgac gtttcgccgt ggcaaagcct ggcgaaagta cgcaatgccc
     9601 tgctgctggc gctgctcaca accgtggcgg catatctgat tatgatgctc gcccccttcc
     9661 ccggcattcg ccagatggcg atttttgccg ccgtcgggtt gagcgcctct tgtctgaccg
     9721 tcctgttctg gcatccgtgg ctgtgccgtg gtctgccggt gcgcccggtt ccggcgatgg
     9781 cgctgatgct acgctggctg gcagcgtggc ggcgcaataa aaaactgtcg cggggtctgc
     9841 ccgttgcgct ggcgctgttt tcgctggcgg ggatgtcaat gctgcgcgtc gatgacgata
     9901 tctcgcagtt acaggcgctg ccgcagcaca ttctggcgca ggagaaagcc attaccgccc
     9961 tgaccggaca aagcgtcgat caaaaatggt ttgtagttta cggcgactcc ccacagcaaa
    10021 cattgcggcg actggagaaa tataccgcct cacttgagta tgcgaaaaaa gaggggctta
    10081 tcagcaacta ccgcactatt ccgctgaact ccctggcgcg gcaggaggaa gacttagacc
    10141 tgctgaaaac ggctgccccg acggtaacaa aagcactgca aaatgcaggg ctgacggcgg
    10201 tgaaaccaga tctcaacgcc atgccggtga aggtcgatga atggctggca agccccgcca
    10261 gtgaaggctg gcgtctgctg tggctgacgc tggaaaacgg cgaaagcggt gtactggtgc
    10321 cagttgaagg ggttaaaagt agcgcattgt tgcaggaaat cgccacatat tacccttgcg
    10381 gcattgcctg ggttgatcgc aaaagcacct ttgatgaatt gttcgcactt taccgctacg
    10441 tcttaaccgg cttgttgctg gtggcgctgg cagtgattgc ctgcggcgca gtggcccgtc
    10501 tcggctggcg caaagggctt atcagcctgg tgccttcggt gctttcgctg ggctgtggtc
    10561 tggcggtgct ggcgatgagc gggcaggcgg tgaatctctt ttcgctgctg gcgctggtgc
    10621 tggtgcttgg catcggtatc aactacacgc tgtttttcag taatccgcgc ggtacaccgt
    10681 taacttcgct actggcgatc gcgctggcaa tgctcaccac cttgctgacg ctgggaatgc
    10741 tggtattcag cgccacccag gccatcagca gttttggcat tgtgctggtg agcggtattt
    10801 tcaccgcctt cctgctttcg ccgctggcta tgcccgataa aaagagaaca aaaaaatgat
    10861 caaatccaca ttctggcgag cgttcgccct gaccgctacg cttatcctca ccggctgtag
    10921 ccactcgcaa ccagaacagg aaggccgccc gcaggcgtgg ctgcaacctg gcacgcgcat
    10981 cacgctgccg gcaccaggga tttcacccgc agtcaattcg cagcaactgt tgaccggcag
    11041 cttcaacggc aaaacccagt cgctgttagt gatgcttaac gccgacgatc agaaaatcac
    11101 ccttgccggg ctgtcgtcgg tcggcattcg cctgtttctg gtgacctacg atgcgcaagg
    11161 gctacgcgcc gagcaatcca tcgtcgtccc acagttaccg cccgcaagtc aggtactggc
    11221 tgacgtgatg ctcagccact ggccgattag cgcctggcaa ccgcaacttc ccgcaggatg
    11281 gacacttcgc gacaacggtg acaaacgcga gctgcgtaac gccagcggca aactggtcac
    11341 ggaaatcacc tatctgaatc gccagggaaa acgcgtgcca atcagcattg agcagcatgt
    11401 ctttaaatac cacatcacca ttcaatactt aggtgactga tatgatttat atttccgccg
    11461 tcggcatgat caacgccctg ggaaataacc ttgacgaaat cgcagccaac ctggctcgcg
    11521 gtgtcgcccc cggcatgcgc ccgcgcgcag gctggttgca ggggcatccg caggcggtcc
    11581 tggctggcgt ggatggcgag cttccgctta tcccggaaaa attcgctgcg catcgctcgc
    11641 gcaacaatca gatcctgctg gcggcgctgg cgcagctcca gccgcaggtt gatgacgcta
    11701 tcgcgaaata tggctgcgag cgcatcgcga ttgtgcttgg caccagcacc tccggcctgc
    11761 atgaaggcga cacgcacgtt aacctgcaca cccacgggca gccaagcacc acgtggcact
    11821 atgcacaaca agaactcggc gatccgtcgc gttttctcag ccactggctg gcgctcgacg
    11881 gcccggccta taccctttca accgcctgct cttccagcgc cagagcgata atcagcgggc
    11941 gcaggcttat cgaagcgggg ctggtcgatg ccgccattgt gggtggcgca gacaccctga
    12001 gccggatgcc gattaacggt tttcacagcc tcgaatcgct gtcgccaacc ttatgtcagc
    12061 cgtttggtcg tgaccgcgca ggtatcacca ttggtgaagg ggcggggcta atgctgctga
    12121 cccgggaacc gcagcccatc gcgttgctgg gcgttggtga atcgagcgac gcttaccata
    12181 tttcagcccc gcatccgcag ggcgaaggag cgatccgcgc tattaatcag gcgctgaccg
    12241 atgcgcagct tacgccagat gacgtcggct atatcaacct gcacggcacc gccacacaac
    12301 tcaacgatca gattgaatca atggtggtta acgccctgtt tggcgaacgc gtaccgtgca
    12361 gctccaccaa acatttgacc ggacacacgc tgggcgcagc gggcattacc gaggcggcaa
    12421 ttagtatgtt gattttacag cgtgatttac cgctgcccgc tcaggacttc agcctgtcgc
    12481 cgcgcgatcc cacgctgcca ccgtgtggca ttatcgaaaa accgcagccg ctggcacgtc
    12541 cggtgatcct gtccaattcc ttcgcctttg gcggcaataa cgccagcatt ctgctcggga
    12601 gggtttcgtg agccactatt tatcccccgg cgcttatctg ccccacgacg cgcccatgtt
    12661 actgctggaa gacgtggtga gcgtcagcga tgacagcgcg gtgtgccgcg tgacggtttc
    12721 gcccagtggc gtactggcac cgtttctcga cccggacggc aatctccctg gctggtttgc
    12781 ccttgaactg atggcgcaaa ccgtcggcgt ctggtctggc tggcatcgcc accagcaggg
    12841 caaaaacagt attgagttag ggatgatact gggcgcgcgt gaattgcttt gcgccgctgg
    12901 cattctgccc gcaggccaga cgctgaccat taccgtcaaa ctgctgatgc aggacgatcg
    12961 cttcggcagt tttgagtgca gcatcaacga cggcgaggcg acgggccgca ttaacacctt
    13021 ccagccgacc gcggaagaac ttaccaccct gtttcaacaa ggagcgtcct gatgagtcgt
    13081 tcagttctgg ttactggtgc cagcaaaggc attggtcgcg ccatcgcctg ccaactggcg
    13141 gcagacggct ttaacattgg cgttcactat caccgtgacg ccacaggcgc tcaggaaacg
    13201 ctgaatgcca ttgtcgccaa cgggggtaac gggcgtttgc tcagtttcga cgtcgcaaac
    13261 cgcgaacagt gtcgggaagt gctggagcat gagatcgcgc aacacggtgc ctggtacggc
    13321 gtggtcagta acgccgggat cgcccgcgat gccgcttttc cggctttaag cgatgacgac
    13381 tgggatgcgg tgatccacac caacctcgac agtttctaca acgtcattca gccgtgcatt
    13441 atgccaatga tcggctcgcg ccagggtggg cgcatcatca cgctgtcctc ggtttccggc
    13501 gtaatgggca atcgcggcca ggtgaattac agcgcagcca aagccggaat tatcggcgca
    13561 acaaaagcgc tggctatcga actcgcgaag cgcaaaatca ccgtcaactg catcgcgccg
    13621 gggcttattg ataccgggat gatcgaaatg gaagaaagcg ctctgaaaga ggcaatgtcg
    13681 atgatcccca tgaaacgtat gggccaggcg gaagaagttg ccgggcttgc cagttattta
    13741 atgtctgata ttgcgggtta cgtcacccgc caggttattt ccatcaacgg agggatgtta
    13801 tgacacgtcg cgtagtgatt acaggaatgg gcggcgtgac tgcctttggt gaaaactggc
    13861 aggacgtttc tgcacgctta ctggcgtatg aaaatgcggt gcgcaaaatg ccggagtggc
    13921 aggtctacga tgggctgcac accctgcttg gcgcgcctgt tgatgatttc acgctaccgg
    13981 agcactacac ccgcaagcgt atccgtgcga tgggccgcgt gtcgcaaatg tcgacccgtg
    14041 ccagcgagct ggcgttagag caggcagggt tgattggcga tccgatatta accagcggcg
    14101 aaacgggcat tgcgtacggt tcatcgaccg gcagtaccgg cccggtaagc gagttcgcca
    14161 ccatgctgac ggaaaagcac accaataaca tcactggcac cacctatgtg cagatgatgc
    14221 cgcacaccac cgccgttaat actggcctct ttttcggcct gcgcgggcgg gtgatcccaa
    14281 cctccagcgc ctgtacttcc ggcagccagg caatcggtta tgcgtgggaa gccattcgcc
    14341 acggttatca aacggtgatg gttgctggtg gtgccgaaga gttatgtcct tcagaagcag
    14401 cggtatttga tacgcttttc gccaccagcc agcacaatga tgcgccgaaa accacacctt
    14461 caccgttcga tgaaaaccgc gatgggctgg tgattggtga aggcgcaggt acgctcattc
    14521 tcgaagaact ggagcacgcc aaagcgcgcg gcgcgaccat ttacggtgaa atcgtcggct
    14581 ttgcgacgaa ctgcgacgcg gcacatatta cccagccgca gcgcgaaacc atgcagtatt
    14641 gtatggagca atcgctgaaa atcgccgggt taagcgcaca agatatcggc tatatttcag
    14701 cgcatggtac ggcgaccgat cgcggtgata tggcagaaag tctggcgaca gcaaccattt
    14761 acggtgataa tgtgccgctc tcctcgctga aaagttattt tggtcatacg cttggtgcct
    14821 gtggcgcgct cgaagcctgg atgagtctgc aaatgatgcg cgaaggttgg tttgcgccta
    14881 cgctaaacct gaataaacca gacccgaatt gtggcgcgct ggattacatc atgcatgaag
    14941 cccgcaaggt tgattgtgag tttttgcaga gtaacaactt tgcctttggc ggcattaata
    15001 catccatcat tattaagcgc tggccctgag ttatacaacc acatggacat cccatgtggt
    15061 gattcttcaa aaaatctgaa tcaatgaata tgaacctcaa attctcttac gcatactgaa
    15121 gagcattccg tataacgatt cagcagacaa aactttatca gtccacacga atcaacggat
    15181 gcacagatga cacgcccggt tatcaccggg caatggttgc cataacgggt aacataaggg
    15241 actgaacagt tgtctttagc gcaacgaact gatctaacgt acctgaacgg acaacctgaa
    15301 aacagccttc catcctgcat tgtccgccag tgcttattac cagccacgtc tgttgcagta
    15361 aattaatcag ttgagaacat tcttcatcaa tcgtgacgct ggctcgcgca tggcagaaaa
    15421 ggtcctgact gagcaacaac aggcatcgta ctgttgctgc tccatgttgt ttatatgaac
    15481 gcatcccgtt tgcaaggctt ccgtgttttt gacgttgacg gtagattgct gctctatatc
    15541 cttattgcag ccggtgctgc gggcctcaat gagattcgct gactctcgcc ttattcacgc
    15601 aacgagcttt aagctcagaa aaacatccaa gtttggtgag tcacagtctt acctgtcgtt
    15661 gtcatcaata taggaatgga taatctggag agcgatactg caggctgata ttgcggtttt
    15721 gcgacaagtt tttagcatcc gcttctacgc caaatacctg aagtaggaat ggatgcgtta
    15781 gcagcataca tccggaactg cgcggggaat agccggaaac ggctatccct accacaatag
    15841 ctaactttgt aatagcgctc ttttaggctc atgtactctg acgtaattct atctggtagt
    15901 tgaattgcac gatacgcgac tcttctgatt catcatgatt attattcaat agtccaatca
    15961 gtaacttacc tgtctctatc cccatttttt tgtagtcaat acggatggat gtcaaagttg
    16021 ggtgtgtttg atcgcaattg tctgaaccgt caagacaagc tacagcgata tttccaggga
    16081 tttttaacaa acgccgctgg cactcaaaca ttatacccag cgcaatctct tcgtggctgc
    16141 aaatcacagc atccagctct ggttgtcgca gtaatatctc gtttaatgcg taacgcccca
    16201 attgcagact tgcggcatcc ggcatagtga taacaagatc agtgttctgg taatggctca
    16261 gcattgcttt atgccagccg ttcagttgct gacgttgcag gcggttgtcc atatgcgcac
    16321 cgataaatcc gatattctta taaccctgct ccagcagatg ttcggtcagt ttatgtgatg
    16381 cttcaaaaaa tgctgtttcg atggtaattt gagccggact gaaaggggaa ccgataacgt
    16441 taatggtagg tatatttgtt ttctcgagta tctggtgagt tttatcagcc aactgagaac
    16501 caaaaataac taacgccgcc gggttgcctt gaagaagcgt catcagaatt tcagcttctt
    16561 tgagctgatt atactcatga cacccaagca gtagctggaa ttcattttta ttaagcacct
    16621 gctgtaatga ctgcataaag cgggagctgg ctttatctgt cagggaagga accagtacgg
    16681 cgactgtatg actgtgacca gatgcaaggg cgccagcagt tctgttggga atataaccca
    16741 actcttctac ggcctgttct atcttttccc tcagcttgtc agaaacctgt tccggtgttc
    16801 tcagcgcgcg ggaaacggtc atggttccga cacctgcaaa gttagcgacc tcctgcagcg
    16861 tcacccgtcc ggtaccacgg cgcttacgtg ttttttccat ttcgactcca tggataatta
    16921 caaccagctg ttttcattac ttttatttca ccattactct ttccagtttt tgcccacggt
    16981 agcaaatatc ctgttgcatt gtatatgcag gaaccgaata tagccaaagc atggcggcag
    17041 aaaaatgata gcgctatcac aaagattggt agcgttatca gatagcgcta tcaaatgcga
    17101 caatcatcac attaattaat gcgtaaaata tctactctcc tgttaattat catcgggagt
    17161 tatcagtagc atgttaaaaa atctattaaa tacagaggtc gttcaggtcg tggaacaggt
    17221 caaagactgg cgggaagccg tggcgatttc atgccgaccg ttgattgaaa acggctctat
    17281 cgaaccgcgt tatgttgatg cgatctatca ctcgcacgac actatcggtc cgtactatgt
    17341 ggttggccct ggtattgcga tgcctcatgc gcgcccggaa gagggggcta acaagctgtc
    17401 attagcactg accctgatcc cttcaggtgt gaattttgat gccgatgaaa acgatccagt
    17461 gaaattactg attgtgctgg ctgcaaccga cagtaccagc cacattgaag cgatttctca
    17521 actcgccaag ttattcgata acgaaaaaga catacaggcc attttgacgg ccaaaacgac
    17581 gcaggacatt ttgtctgtta ttgcgcgcta ttaatccaga ggaacattta tgaaaattac
    17641 agtggtttgc ggtaacggct taggcaccag cctgatgatg gaaatgagca ttaaaagcat
    17701 tttgaaggat ttgtccgtca gcgctgatgt tgatcacgtc gacctgggct ccgcgaaagg
    17761 gacgccgagt gatatctatg tcggcaccaa agacattgcc gagcagttag ccgcgcagtc
    17821 tgtcgcaggc aaaatcgtcg cactggaaaa tatgattgat aagaaagcca tgagagaacg
    17881 tttgtccgtg gtattaaccg aattaggcgc actgtaagct gggggcgata tggatttctt
    17941 tcgttttttg atgagcgatg tactttccga acccgcagtg ctggtcggtt taatcgccct
    18001 gattggcctg attgcacaga aaaaaccggt aaccgaatgt attaaaggta ccgtcaagac
    18061 cattatgggg tttgtgattt taggcgcagg ggcgggttta gttgtgtcgt cactgggtga
    18121 cttcgcaaac attttccagc atgcctttgg tattcagggt gtagtgccga acaatgaagc
    18181 cattgtttcc gtagcgcaga aaagcttcgg gaaagaaatg gcgatgatta tgttctttgc
    18241 gatggttatc aacattatga ttgcccgttt cacaccgtgg aaatttatct tcctgaccgg
    18301 tcatcacacg ttgtttatgt cgatgatggt agcggtaatt ctggcaacag caggcatgac
    18361 cggcattacg cttatagccg ttggctcttt ggttgttggc gtggcaatgg tctttttccc
    18421 ggctattgcg catccgtaca tgaagaaagt gaccggttct gatgacgttg ccatcggcca
    18481 cttctcaaca ttatcttatg tattggccgg tttcatcggc agcaagtttg gtaataaaga
    18541 gcactcgact gaagacatga atgtgccgaa aagtctgctg ttcttgcgcg acacgccggt
    18601 tgctatctct tttaccatga gcataatttt cctggtgacg tgcctgtttg cgggtgcaga
    18661 tgcggtgaaa gagcttagcg gtggtaaaaa ctggttcatg ttctctatca tgcaatccat
    18721 taccttcgca gctggcgtgt acatcattct gcagggtgtg cgcatggtga ttgcggagat
    18781 tgtcccggca ttcaaaggta tttcagacaa gctggttccg aatgccagac ctgctctcga
    18841 ttgcccggtc gtcttccctt acgcacctaa cgctgtactg gttggtttcc tgagtagctt
    18901 tgctgcaggt ttaatcggta tgttcacgtt gtatctgctg aacatgatcg tgattattcc
    18961 tggtgtggtg ccgcacttct tcgtaggtgc agctgcaggg gtattcggta atgcaacggg
    19021 tggacgtcgt ggtgcgattt tgggtgcctt cgctcagggc ttgcttatta ccttcctgcc
    19081 agtattctta ttgccggtgc tgggtgatat tggttttgct aacaccacct tcagtgatgc
    19141 tgactttggc gcactgggta ttttgttagg gattatcgtt cgctaatatt ttgatgacat
    19201 taatatcctc caggatttca ggaacgtgga ggatattaat tcctcttatt ttctccattt
    19261 ttctttcttc cagtcaggac tgcatccgat taatcgtaag cgctccgaga agtacgtagc
    19321 gtaaggatta ttttacagct gagaagtttc agtgcagcag ttcatgcaca cggttcgacg
    19381 gctagtcatt gatcttcata attacctcgc gtaaaaaacg gggtaaggcc gctcatccag
    19441 cgtcatctcc acaggtaata attcaacgcc aatgccagaa gagttcgtta ccggaagacg
    19501 ccgtgatata cgccctttga tcagcgagag cctgagccat ttgaacagaa gattatcatt
    19561 gatatcatgc tcccgggcaa tacgggcaac agaacccctt ggttgtaaag ccagtttaac
    19621 catttgaagt ttaaactcat ttgaaaatga cctgcggggt tcaactgata atgttttttg
    19681 ttccataaca ggtgtccact agttgaaaag gtgggtacct atgttaccaa tactggctta
    19741 atggctacat acagcagtcg gtttacgcat acacttttat agtgcactcc ggcctcatga
    19801 atataacggt ggattaccgc cgaacgaatc ggaaaataga tactggaaaa aaactgaaat
    19861 tgtgaccagt ttttgttgac cactacaaat aaataaaaga gttgcagtgc ttgctcggta
    19921 agaaaacaat ggaggttaag ctcctcaaag aagctgtgga gtacaccact ctacagtagt
    19981 tgcgcaaatt tggagtggac gataatgtga tcgtctataa gggcaacgct atcatagtct
    20041 tgtcctggcg ggtaaaaaaa cgcgcttacc ttaacgataa gcgcgccgct gttcaggcct
    20101 tgagtggtta ttcaattcct gtggtgactg taaaagtgcg cgtttgctgc ggtgcaacct
    20161 gaatcagcgt gccattacgt tgcgcggcaa gatacccctc aggccgacag gttgcaggta
    20221 atgcaaaggc ggctacctgt tgctctccgt tataaaggat ccagcgtgtc acataattta
    20281 gttcagcact gtagaaacga gtaacaaacg tagtgccatc gggagagatc atgcgaaact
    20341 ctggctgctc tgtataagcg tccagtttgt ctgcaaagaa gacaatttct ggatcataaa
    20401 attccggttg actcagcgtc gacagagagg cttctccctg cataatccgt tgattaaacg
    20461 ccagccactg agcggtggga ttaacatgcg aaggcactga ttcacgcaat cttaatattt
    20521 cgtccgggat attctggctg aatgtagcat ttggtatata tgcataattc atgtggcaca
    20581 tatattgtag tggcatatct acagaagcca gattggttac ggccatctta atatcgaaca
    20641 gtgtagagga tttgtgaagg accactgttg gctgagccag ataatgatga ccgaaaccca
    20701 ttacatactc gtaacgcccg ttaaggcgta acatatctcc gtctaattcc agccatgctt
    20761 catccatcgc ggcacaggcc atttcaccgt gtagcagatg agtatcttcc gcagatgggc
    20821 agccattagc cagcaaacct gaatgaaaag caaaacagcc ataggtctct atcacctctg
    20881 tcgccggttt aggctggcga aacatattgc acatggtgag gccgtgtcca tcaaattgcg
    20941 catcccaaat catctgcccc atccagggaa gaataatcaa atgtccacga ctgtttgcaa
    21001 ttttaagccc ctcgacaccg ctgtcatatc gaaaagacgt gacagtaaaa tcactatttt
    21061 ccagcaagat acgaggtttc tcgccaaaaa gcgcccgcca caaattaata cgcgtactca
    21121 taacggttct cctcaggacg ctgtgacttc agccagtgcg gtacgtactt tgctttcacg
    21181 ccagaagtag actccgacat agacaaagca gagcatagaa accaggaatg aaagctgtag
    21241 tgagtggaac atatctgcaa tatatccctg aattgccgga accaccgcgg caccgacaat
    21301 agccataaca atgactgctc ctgccatttc tgtatgttcg ttatcaactg tatccagtgt
    21361 tcctgcatag atcgtcgccc agcaagggcc aaacaaaaca cttaccagga cggcgacata
    21421 gaccgcgctg aaacttggag ccagtgcaac atatgccagg aacagcgccc ctataacgga
    21481 atagagaatc aatacttttt ccggattaaa acgcgtcata aggatattgg ctataaactt
    21541 gccaataaag aagcaggcaa agctatagac catgaagttt gaagcatcac gttcgttgat
    21601 atcgcccaac tccagcgcca gacggatggt aaatgaccat actgcgacct gcatacccac
    21661 ataaaggaac tgcgccacaa taccgcgacg aaagcgcgga tttctagcca gatagcgcag
    21721 cgtatccatt gctgacgggc gtttatggtg acttgtctgt gccactttac aggttgggaa
    21781 gcgggttaaa aggaacaaca ccatgaccac aactagaatc ataatcatat acttatacgg
    21841 ttcaagggtg ttctctaaca tcagcacctt aaagttgtga atttgctcgg cgttcattcc
    21901 tgacatctgc ttttcaaggc tttccccctc ggagaaaacc agatatttgc ccaataaaat
    21961 accagacgca gcaccaatcg gataaaaggt ctggctgata ttgagccgca atgtggcata
    22021 ggcttttgga ccgatcattg aactgtatgt gttcgctgca gtttcaagga aactcaggcc
    22081 aatcgcaatc gcaaaaatag ctgcaaggaa catagtgtag gttgccatat gcgaggcagg
    22141 gaaaaaaagt gtacaaccac caatatacag cgtcaggcca attaaaattg ccaccttata
    22201 actggtcttt ttaatcacaa gggatgctgg tattgcaatt aaaaaataac ctccataaaa
    22261 tgcgctctgc accaatgctg aagcaaagtt acttagcgaa aatacacttt tgaattgagt
    22321 gattaatatg tcatttaatg cagctgcgca tccccatagc gggaataaac acgataacaa
    22381 aataaactgg aacaagggag tcttattcag atacccatca ggcatctgaa tgatgttttt
    22441 atcgttcata gtgctacctt taactgtgca ggatgattat tcgtttaagg ttaaaaattc
    22501 attaaattgt tcaatactcg gataagatga ttgcgtacct ttccctgtga cgctgaaagc
    22561 ggcaaagaga gcggcttttt tcaaagcggc ttcaacatca ccgctttgaa cataataatg
    22621 ggaaaagcaa ccaataaatg cgtcaccagc gccactagta tcaacagcat ttactttgaa
    22681 tgcaggaaca tggacttcct gatcgcgggt catccataat gcgccttttt cgctcatggt
    22741 aacaataata ttgttcagcc ctttatcaac taacgaacgt gcggccaaac gaatatgatc
    22801 ataagtatca accgacatac cggttaatat ttccagttct gtttcattcg ggataaagaa
    22861 atcacatttg caggcataag acatatctaa ctcacgcaat gccggagccg gatttaataa
    22921 cacttcaata ccatttttct taccaaactc aatcgcgtgg taaactgttt ccagttgaac
    22981 ttccagttgt aaaacgatca atttgcattt tttcagatct tctgcagctc gatcgatatc
    23041 ttccggggaa agaaatttat tcgctccctt aattattaat atactattgc tcgagttggc
    23101 attaacaaag atcggtgcaa caccactgct ggtacagggg actttctcaa cataagtggt
    23161 attaattccc catgattcga gattacgaat agtattatcc gcaaaaatat catcacctac
    23221 tttagtcagc atcaggactt ttgaattcaa tttagccgcc gccaccgctt gattagcacc
    23281 tttcccacca catccgattt tgaaggcagg tgcttccaga gtttctcctt ctttaggcat
    23341 ctgattagtg taagtaatga gatccaccat attggaacca ataactgcaa tgtccatttc
    23401 actacctctt ataaactttc gcataacaat ggtatttaaa taacattagc atgttacttt
    23461 tacatcattt gtgactgaga tcgcgattag cacatcaacc cgatgtttat ttaatagact
    23521 tccagtctta tcactcaggc caacactatc taatcataag caacctaaca gg
//
LOCUS       DAPHDW010000067        23494 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.067, whole genome shotgun sequence.
ACCESSION   DAPHDW010000067 DAPHDW010000000
VERSION     DAPHDW010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 23494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.067"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(11..331)
                     /gene="chbB"
                     /locus_tag="RH057_002557"
     CDS             complement(11..331)
                     /gene="chbB"
                     /locus_tag="RH057_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HDV3128910.1"
                     /translation="MEKKHIYLFCSAGMSTSLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(630..968)
                     /gene="osmE"
                     /locus_tag="RH057_002558"
     CDS             complement(630..968)
                     /gene="osmE"
                     /locus_tag="RH057_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HDV3128911.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            1170..1997
                     /gene="nadE"
                     /locus_tag="RH057_002559"
     CDS             1170..1997
                     /gene="nadE"
                     /locus_tag="RH057_002559"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HDV3128912.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETGNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLAALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNVPEQVARTIENWYLKTEHKRRPPITVFDDFWKK"
     gene            2227..3114
                     /gene="cho"
                     /locus_tag="RH057_002560"
     CDS             2227..3114
                     /gene="cho"
                     /locus_tag="RH057_002560"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HDV3128913.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLRSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(3074..3649)
                     /gene="ves"
                     /locus_tag="RH057_002561"
     CDS             complement(3074..3649)
                     /gene="ves"
                     /locus_tag="RH057_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HDV3128914.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMFLESADRFNHTLKPLQPFAFAADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGACWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(3852..4337)
                     /gene="spy"
                     /locus_tag="RH057_002562"
     CDS             complement(3852..4337)
                     /gene="spy"
                     /locus_tag="RH057_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HDV3128915.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIREIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(4667..5635)
                     /gene="astE"
                     /locus_tag="RH057_002563"
     CDS             complement(4667..5635)
                     /gene="astE"
                     /locus_tag="RH057_002563"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HDV3128916.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDLGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSARHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(5628..6971)
                     /gene="astB"
                     /locus_tag="RH057_002564"
     CDS             complement(5628..6971)
                     /gene="astB"
                     /locus_tag="RH057_002564"
                     /EC_number="3.5.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
                     /protein_id="HDV3128917.1"
                     /translation="MNAWEVNFDGLVGLTHHYAGLSFGNEASTRHRFQVSNPRQAAKQ
                     GLLKMKALADAGFPQAVIPPHERPFIPVLRQLGFSGSDEQVLEKVARQAPHWLSSVSS
                     ASPMWVANAATIAPSADTLDGKVHLTVANLNNKFHRSLEAHVTESLLKAIFNDEEKFS
                     VHSALPQVALLGDEGAANHNRLGGHYGEPGMQLFVYGREEGNDTRPSRYPARQTREAS
                     EAVARLNQVNPQQVIFAQQNPDVIDQGVFHNDVIAVSNRQVLFCHQQAFARQSQLLAN
                     LRARVNGFMAIEVPATQVSVSDAVSTYLFNSQLLSRDDGSMMLVLPQECREHAGVWGY
                     LNELLAADNPISELKVFDLRESMANGGGPACLRLRVVLTEEERRAVNPAVMMNDTLFN
                     ALNDWVDRYYRDRLTAADLADPQLLRGGREALDVLSQLLNLGSVYPFQREGGGNG"
     gene            complement(6968..8446)
                     /gene="astD"
                     /locus_tag="RH057_002565"
     CDS             complement(6968..8446)
                     /gene="astD"
                     /locus_tag="RH057_002565"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HDV3128918.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLERNKGELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHARTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLARLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQSETSLLTPGIIEMTGVAGVPDEEVFGPLLRVWRYDSFEEA
                     ILMANNTRFGLSCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(8443..9477)
                     /gene="astA"
                     /locus_tag="RH057_002566"
     CDS             complement(8443..9477)
                     /gene="astA"
                     /locus_tag="RH057_002566"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HDV3128919.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(9474..10694)
                     /gene="astC"
                     /locus_tag="RH057_002567"
     CDS             complement(9474..10694)
                     /gene="astC"
                     /locus_tag="RH057_002567"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
                     /protein_id="HDV3128920.1"
                     /translation="MSQPITRENFDEWMIPVYAPAPFIPVRGEGSRLWDQQGKEYIDF
                     AGGIAVNALGHAHPELREALNEQASKFWHTGNGYTNEPVLRLAKKLIDATFADRVFFC
                     NSGAEANEAALKLARKFAHDRYGSHKSGIVAFKNAFHGRTLFTVSAGGQPAYSQDFAP
                     LPADIRHAAYNDINSASALIDDSTCAVIVEPIQGEGGVVPASNAFLQGLRELCNRHNA
                     LLIFDEVQTGVGRTGELYAYMHYGVTPDLLTTAKALGGGFPVGALLATEECARVMTVG
                     THGTTYGGNPLASAVAGKVLELINTPEMLNGVKQRHDWFVERLNTINHRYGLFSEVRG
                     LGLLIGCVLNADYAGQAKQISQEAAKASVMVLIAGGNVVRFAPALNVSEEEVTTGLDR
                     FAAACEHFVSRGSS"
     gene            11140..11946
                     /gene="xthA"
                     /locus_tag="RH057_002568"
     CDS             11140..11946
                     /gene="xthA"
                     /locus_tag="RH057_002568"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HDV3128921.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGLVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            12113..12823
                     /locus_tag="RH057_002569"
     CDS             12113..12823
                     /locus_tag="RH057_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV3128922.1"
                     /translation="MNAERKFLLACLIFALVIYAIHTFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFIIATLFLLPGSILVIAGGIVFGPLLGTLLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGLTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILVQLAKLYARHKH
                     VDLSASRRSPLTHPKNEG"
     gene            12828..13505
                     /gene="ydjY"
                     /locus_tag="RH057_002570"
                     /pseudo
     CDS             12828..13505
                     /gene="ydjY"
                     /locus_tag="RH057_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
     gene            13520..14227
                     /locus_tag="RH057_002571"
     CDS             13520..14227
                     /locus_tag="RH057_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HDV3128923.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTGLFILFALTV
                     VIFMAKKIWLERQKRNA"
     gene            14227..14775
                     /locus_tag="RH057_002572"
     CDS             14227..14775
                     /locus_tag="RH057_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HDV3128924.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            14785..15951
                     /locus_tag="RH057_002573"
     CDS             14785..15951
                     /locus_tag="RH057_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3128925.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NFRTLKEANLLQTGWAETLPNWRYVDTQLPVREDFSVPTEGAESPWGGAQLTFIARRD
                     VTPQPPQTPQALLEFAKANPGTVTYPRPPDFTGTAFLEQLLIMLTPDPAALKVAPDKA
                     TFARVTAPLWQYLDALHPYLWRKGKDFPPSPARMDALLKAGTLRLSLTFNPAHAQQKI
                     ASGDLPASSYSFGFNQGMIGNVHFVTIPANANASAAAKVVANFLLSPNAQLRKADPAV
                     WGDPSVLDPQKLPDGQRETLQSRMPQDLPPVLAEPHAGWVNALEQEWLHRYGTH"
     gene            15924..17459
                     /locus_tag="RH057_002574"
     CDS             15924..17459
                     /locus_tag="RH057_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HDV3128926.1"
                     /translation="MATPLRYALIFLLWAMVAVIYAPLIPAALTLISPALSLTHWQAL
                     FADPQLPQALLATLVSTTIAAVGALLIALLVIVALWPWPKWQRMCARLPWLLAIPHVA
                     FATSALLLFADGGLLYDYFPYFTPLMDKLGIGLGLTLAVKESAFLLWILAAVLSEKRL
                     LQQVIVLDSLGYSRWQCLNWLLLPSVAPALAMAMLAIVAWSLSVVDVAIILGPGNPPT
                     LAVISWQWLTQGDADQQTKGALASLLLMLLLAAYVLLSYLLWRSWRRTIPRVDGIRKP
                     ATPLLPGTTLASFLPLTGVLCVVLLAILADQSTINSEALINSLTMGLVATFIALLLLL
                     LWLEWGPQRRQLWLWLPILLPALPLVAGQYTLALWLNLDGSWTAVVWGHLLWVMPWML
                     FILQPAWQRIDSRLILIAQTLSWSRAKIFFYVKCPLMLRPTLIAFAVGFSVSIAQYMP
                     TLWLGAGRFPTLTTEAVALSSGGSNGIFAAQALWQLLLPLIIFALTALVAKWVGYVRQ
                     GLR"
     gene            17459..18112
                     /locus_tag="RH057_002575"
     CDS             17459..18112
                     /locus_tag="RH057_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HDV3128927.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     STLKGTARRNAVKDALDRAGLAETYHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRELAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            18179..19486
                     /gene="ynjE"
                     /locus_tag="RH057_002576"
     CDS             18179..19486
                     /gene="ynjE"
                     /locus_tag="RH057_002576"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
                     /protein_id="HDV3128928.1"
                     /translation="MKRVSQMTALAMALGLACASSWAAELAKPLTPDQLQQQNGKAID
                     TRPSAFYNGWPQTLNGPSGHEPAALNLSASWLDKMSTEQLNAWIKQHNLKADAPVALY
                     GNDKDVDAVKTRLQKAGFTHISILSDALSEPSRLQKLPHFEQLVYPQWLHDLQQGKEV
                     TAKPAGDWKVIEAAWGAPKLYLISHIPGADYIDTNEVESEPLWNKVSDEQLKAMLAKH
                     GIRHDTTVILYGRDVYAGARVAQIMLYAGVKDVRLLDGGWQTWSDAGLPVERGTPPKV
                     KAEPDFGVKIPAQPQLMLDMEQARGLLHRQDASLVSIRSWPEFIGTTSGYSYIKPKGE
                     IAGARWGHAGSDSTHMEDFHNPDGTMRSADDITAMWKAWNIKPEQQVSFYCGTGWRAS
                     ETFMYARAMGWKNVSVYDGGWYEWSSDPKNPVATGERGPDSSK"
     gene            complement(19495..20115)
                     /locus_tag="RH057_002577"
     CDS             complement(19495..20115)
                     /locus_tag="RH057_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HDV3128929.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPQQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLKSHQRQ"
     gene            20202..20609
                     /gene="nudG"
                     /locus_tag="RH057_002578"
     CDS             20202..20609
                     /gene="nudG"
                     /locus_tag="RH057_002578"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HDV3128930.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVEPDES
                     QRQALVRELREELGIEATVGEYVTSHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARPAD"
     gene            complement(20632..20847)
                     /locus_tag="RH057_002579"
     CDS             complement(20632..20847)
                     /locus_tag="RH057_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1496 domain-containing protein"
                     /protein_id="HDV3128931.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDKNYSEGAVIKAEGILLQC"
     gene            21083..22426
                     /gene="gdhA"
                     /locus_tag="RH057_002580"
     CDS             21083..22426
                     /gene="gdhA"
                     /locus_tag="RH057_002580"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HDV3128932.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQMSLLERLVEPERVIQFRVVWVDDRNQVQVNRAWRVQFSSAIGPYKGGMRFHPSVN
                     LSILKFLGFEQTFKNALTTLPMGGGKGGSDFDPKGKSEGEVMRFCQALMTELYRHLGA
                     DTDVPAGDIGVGGREVGFMAGMMKKLSNNTACVFTGKGLSFGGSLIRPEATGYGLVYF
                     TEAMLKRHGMGFEGMRVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDESGFTK
                     EKLARLIEIKASRDGRVADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDVDAAHQ
                     LIANGVKAVAEGANMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQNAARLG
                     WKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQGVI"
     gene            complement(22543..>23494)
                     /locus_tag="RH057_002581"
     CDS             complement(22543..>23494)
                     /locus_tag="RH057_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416276.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV3128933.1"
                     /translation="IKENKAMLLCKWGFYLTCVVAVMFVFAAITSNGLNERGLITAGC
                     SFLYLLIMMGLIVRAGFKAKKEQLHYYQAKGIEPLSIEKLQALQLIAPYRFYHKQWSE
                     TLEFWPRKPEPGKDTFQYHVLPFDSIDIISKRRESLEDQWGIEDSESYCALMEHFLSG
                     DHGANTFKANMEEAPEQVIALLNKFAVFPSDYISDCANNRSGKSSAKLIWAAELSWMI
                     SISSTAFQNGTIEEELAWHYIMLASRKAHELFESEEDYQKNSLMGFLYWHICCYRRKL
                     TDAELEACYRYDKQFWEHYSKKCRWPIRNVPWGASSVKYS"
BASE COUNT         5623 a         6214 c         5979 g         5678 t
ORIGIN      
        1 ttaaaataaa ttaatttgct gcggcttttt taatcgctgc aaccgcagcc ttaagcacgc
       61 ctaaaccatc gactttgcca taaagcagcg agtcaattac ttcaaccggt ttgttgggta
      121 acaaacgctg gatttcgggc aacatataag caatctgcgg ccctaataac acgacatcgg
      181 cattctgacc tttttcacca gccagtgttt ccggaaatgc ttcaataatg accggaactt
      241 catatttttc tgcctgtgcg cgcatttttg ataccagtaa agaggtagac atgcccgcag
      301 aacaaaacag ataaatgtgt ttcttttcca taaaactgcc ctcatcgacg attatctgtc
      361 agccagacac tccgcaagcc ttaacctgct tccatgctct gggtaacttg cgaaaccaac
      421 atgacttttt ttgtggctga aaggagtata cggtatagac ctgagcaata gtatttcttt
      481 gaccttataa attgtctctc cttgacctgc ggttatgacc ctcttcacat ttcgggcaaa
      541 taattcgcga agataattaa gaactcaggc caaaaaaaac cggcgcaatg gccggtttcc
      601 gttgtaactc aagctttcag gcgaagtgat tacttcgcag cctgtggatc agtgtcgtat
      661 tcagcacagg tctgataacc ggagttgatg acatgtccgg tatcatctaa cgcgacaaag
      721 taggtttctg ctttaccatc acgttgaccc aggatgtagg tctggcaagt accacgagca
      781 tggatcatgc tcacttcaga cgaaggctta cctgcaattt gtgcaacctg cgcccggctc
      841 atgccttttt tgacgtcttt caccacaggc tgtacaaact ggtctttagt acgatcataa
      901 gccgtacaac ccgccagcat ggttaatacc gccgctgcac tcagaattcc tgccatattc
      961 ttgttcatat tccgtcctct tgtttatcag cgtgttagat aagcctggaa tacattgggc
     1021 gctttttcaa gcccgtgaac gaaacggctc cgctttcaga ggattcctgt atgatgtttt
     1081 aaccaccatt cagcccgctg tcgcttgtcg tttcagtagc aacgggttag ctttaaggaa
     1141 gttttgtctt ttctgtctgg aggggttcaa tgacattgca acaacaaata ataaaggcgc
     1201 tgggcgcaaa accgcagatt aatgctgaag aggaaattcg tcgtagtgtc gattttctga
     1261 aaagctacct gcaaacttat ccgttcatta aatcactggt gctcgggatc agcggcggtc
     1321 aggactccac gcttgccgga aagctgtgcc agatggcgat taatgagctg cgccaggaaa
     1381 ctggcaacga atcactgcaa tttattgccg tacgcctgcc ctatggtgtt caggccgacg
     1441 aacaagattg ccaggatgcc attgccttta ttcaaccgga tcgcgtatta accgttaata
     1501 tcaagggcgc ggtattggct agcgagcagg cattgcggga agcaggcatt gaactgagcg
     1561 attttgtccg tggcaatgaa aaagcgcgtg agcggatgaa agcacaatat agcattgcgg
     1621 gtatgaccag cggtgtcgtg gtgggcaccg atcatgcagc agaagccatt accggattct
     1681 tcactaaata tggtgacggc ggtacggaca ttaatccgct gtatcgtctc aacaaacgtc
     1741 agggtaaaca gttactggcg gcattaggtt gcccggaaca cctttataag aaagcgccaa
     1801 cggccgatct ggaagatgat cgcccttctc tgccagatga agtggcactc ggcgtgacct
     1861 atgacaatat cgacgactat ctggaaggga aaaacgtacc tgaacaggtc gccagaacaa
     1921 tagagaactg gtatctgaaa accgaacata aacgccgtcc gccaattacc gttttcgatg
     1981 atttctggaa aaagtaataa tttgcaccta ttggattgtg cgaaaaagag taatttgttc
     2041 acgccggatg cggcgtgaac gccttattcg acctacaaaa ctatgcaaat tcaatacatt
     2101 gcaggagtcg aataggcctg acaggcgtag cacgtcagac ggtgtaacct ttgtcatcaa
     2161 cccgcctctt ttttaatcgc ctcccgcctg ttacactgga tagataacca gcattcggag
     2221 tcaacagtgg tacggcgttt aacttctccg cggctcgaat ttgaagctgc ggcaatttat
     2281 gaatatcccg aacatttacg ttcattcctt aatgacttac ccacccgacc aggggtgtat
     2341 ctgtttcatg gcgaaagtga caccatgccg ctctatatcg gcaaaagcgt taatatccgc
     2401 agtcgggtgc tttctcattt acgcaccccg gatgaagccg ccatgctacg gcaatcccgg
     2461 cgaattagct ggatctgcac cgcaggcgaa atcggcgctc tgctccttga agcgcgatta
     2521 atcaaagaac aacagccgct gtttaataaa cggttgcgcc gcaatcgcca gctctgtgcc
     2581 ctgcaattaa atgaaaagcg cgtcgatgtg gtgtatgcca aagaggtgga tttttcacga
     2641 gcccccaacc tgtttggcct gtttgccaat aggcgcgcag ctttgcaagc attgcagagc
     2701 atcgctgatg aacaaaaact ttgttatggc ctgttgggac tggaaccgtt aagtcgcggt
     2761 cgtgcatgtt ttcgttcagc gctaaaacgt tgcgccggag catgctgcgg taaagagagc
     2821 catgaggaac atgcgctacg cttgcgccag tctctggagc gtttgcgggt ggtgtgttgg
     2881 ccttggcaag gtgcggtggc actgaaagaa cagcacccgg aaatgactca atatcatatt
     2941 attcaaaact ggctgtggct gggggcggtt aattcgctgg aagaagcgac aacgttaatt
     3001 cggacacccg ccgggtttga tcacgacggt tataaaattc tttgtaagcc gctgctttcc
     3061 ggtaactatg aaattactga acttgatccg gcgaatgacc agcgagccag ttgatttcac
     3121 tgaacaacag tttaccttgc ggttgtaaaa gccgcagggt gtgcctgcca tcgaaccagc
     3181 acgcgccctg gtcggtagtg agcaatttat cgcccagttg ccacgccccg ttaatcacaa
     3241 acaccacccc gccgcgtgag ccaaaggtag taaacgtacg ttcggcaatt ctgactttcg
     3301 ccttacagac atccaggcgt gtcatgatgt tgaaatccat cgacatctgc cctgccgtca
     3361 gtttcgcttt taccacctgg tccgctgcaa aggcaaaagg ctgtaacggt tttaaggtat
     3421 ggttaaagcg gtctgcgctt tcaaggaaca tctcgccgcc ttccagcaac gtcactatcc
     3481 tttccatgcc gggaaacaaa gaaaactccc cattcgccgc gatggatgca atgctggcgc
     3541 gccagtaaaa atcacgtttt gccggtggga aagtgcaaat ttcgcgcgtt tcaccggcag
     3601 cgtttcgcca cagattcacc gacattttac gcatatcaaa gtattccata ctcgctcccg
     3661 gcccgttctt ttcattgtta tcgttcaggt aaccgtggat ggttatcggc aactcgggtg
     3721 atttacgtta gtggtgatca ggcttttttt acaactgttt gagaagagaa aagaaaaccg
     3781 ccgatcctgt ccaccgcatt actgcaaggt agtggacaag accggcggtc ttaagttttt
     3841 tggctgaaag attattcagc agttgcaggc attttacctt ttgccgctgg acgttctgtc
     3901 agacgcttct caaaattagc attaaattgc tttttctgtt ccggcgtcag gatgttgtaa
     3961 attttgttct gggtttccat gtgcgccagc atgttagctt tgcgctgttc ttccattttt
     4021 gcgatctgcg cttcagcttt tgctttatcg aaggtatcgc tggcaatgat gtcatgcatt
     4081 gcgcggcgtt cttccagcgg cggacgtttc atctggtcac gctggccttt catgatttcg
     4141 cggatctgct gtttctgcgc gtcggtcagg ttcaggtctt tgaacatcat gtcctgatgc
     4201 ggaccgaact tgcctttgtg gtgcatcatc ggcttcgcgt cagccggtgc tgcggtagtg
     4261 gtgtctgcgg catgggccag gttagccgcg ccaagagcca gggtagaggc aacaaacagt
     4321 gcagttaatt tacgcatatt ctatatcctt cctttcagtt atttattacg gctttcttaa
     4381 gtagcgtgcc gtgttgacga gattaacttt actgacttta gcgtcaatta atcagagcaa
     4441 cggtaaaaca atgaaagtgt aaaaaacact tttgcgccaa ttatggagaa aaaaagaaaa
     4501 tttgatggag agtgatgaga gaataataca acacgatgat tttgcagaga ttatgaagaa
     4561 ctataccgga tgactggtga taaataaagc aaataaccag gattaatctg tattaattta
     4621 taagaaagca acttaatacc cgcagaatga tttctgcggg taagtattag cttatttttt
     4681 cgagcattaa tcccgcgcgt aatcccaacg ctaccaacgg attagggaat aacacatact
     4741 ctacatcatg ggttacggta aaacgttcct ctccgtcctg cgccagcaat gttcctttct
     4801 caaacggcat aaaattcagc gtgtcacttg ccatatgcat ttcgaaggac ggcgagtggc
     4861 gagtaatttg cgaaaccacc cgataacgga gcggcggtgt tctcacgata ccgacactct
     4921 caccagatag cagcgcagca attgcgctgg cagttactgc aaactggcga agatcgtttt
     4981 gcccaaaggg caacgctttg ccaagttcca gcgtacaggc cagcgcgcca aaatgtctgg
     5041 cgctgaaatg ggtaaacgta ccaccaggtt cctgatggaa caccagcgcc tccagccccg
     5101 ccgcacccag ccacgtcaga aatttctcgt cccagggaat gtcgcgttgc ggtaatacac
     5161 cgaactgcgg atgcaaggag ccacgaattg cggtatgtag atcaaggtgc cagcgcacag
     5221 attctttgcc caggtcataa aaatcttcca ggcactgttc cagttcgcgc gcccgacagg
     5281 tttctccgct ttcagcaaat agctgccaac gaccgccaaa cattcgattc atatcgctat
     5341 ggcaataacg tttcccttgc ttcagcgcag gaggattccc gaggatcacc agcaaccgcc
     5401 aacgtaacgg gatctcgccg tgagatatcg cgccaagcaa cgcgtccagc atctccacag
     5461 gtgccgtctc attaccgtgt attcccgctg aaatcaccag tgcgccttgc ggtggcgtta
     5521 atggcgtcag ttccagcaca ccatcgccca gccagcgcca gcgaacgccg ttgatttctc
     5581 gctcggtgat aaccggtttt ttacccgtta aggtcagagc aagaaaatta tccattgccc
     5641 cctccctcgc gctggaacgg ataaaccgaa ccgagattca gtaattggct caatacatcc
     5701 agtgcttccc gccctccgcg cagcaattgc gggtcggcca gatcggcagc agtaaggcga
     5761 tcgcggtagt aacgatccac ccagtcattg agcgcattaa acagcgtatc gttcatcatc
     5821 accgccggat tcaccgcccg gcgttcttct tctgtcaata ccacccgcaa ccgcaggcac
     5881 gccgggccgc cgccattcgc catgctttca cgtaaatcaa agacttttag ttcgctaatc
     5941 gggttgtcag cggcaaggag ttcattgaga taaccccata ctccggcgtg ttcccgacac
     6001 tcctgaggca gcaccaacat catggaacca tcatcgcggc tcaacagttg gctgttaaac
     6061 agataggtag acaccgcatc cgacacggaa acctgagttg ccggaacttc tatcgccata
     6121 aaaccgttca cccgcgcacg caggtttgcc agtaactgtg actggcgagc gaacgcctgt
     6181 tggtggcaaa acagcacctg gcggttactc acggcaatca cgtcattatg aaaaacgccc
     6241 tggtcgataa cgtccgggtt ttgctgggcg aaaatcacct gttggggatt cacctgattc
     6301 agccttgcca ccgcctcgct ggcttcgcga gtctgtcgcg ccggataacg ggaaggccgg
     6361 gtatcattgc cttcttctcg cccgtagaca aaaagttgca tacccggctc accgtaatga
     6421 ccgccgagac gattgtggtt tgccgccccc tcatcaccga gcaacgctac ctgcggcaac
     6481 gccgaatgaa cgctaaattt ctcttcgtcg ttaaaaatcg cttttaaaag tgattcggtg
     6541 acgtgcgctt ccagcgagcg gtgaaattta ttgttcaggt tggcaacggt gagatgcact
     6601 ttgccatcca gcgtatcggc agatggcgcg atcgttgccg cattggctac ccacattggc
     6661 gaagcggaac tgacgctgga aagccagtgc ggtgcctggc gtgcaacttt ttccagtacc
     6721 tgctcatcgc taccactgaa tcccaactga cgcagcaccg gaataaacgg acgctcgtgc
     6781 ggcgggatca cggcctgggg gaatcccgca tcggcaaggg ctttcatttt cagtaagccc
     6841 tgttttgccg cctgtcgcgg gttagacacc tgaaaacggt gacgggtaga ggcttcatta
     6901 ccaaacgaca ggccagcgta atgatgcgtc agccctacca gcccgtcgaa attaacttcc
     6961 caggcgttca tcgcaccacc tcatcggaaa aatccagccc ggggttaagc gttgcgggca
     7021 aagttaacga gtccgactcc aggctcgcca taggccatgc gcagtaatct gcggcatacc
     7081 aggcgctggg gcgatggtta ccggatgcac caatgccgcc gaatggcgcg gtactggcag
     7141 caccagtaag cggtttgttc cagttaacaa tccccgcccg cgcctccagc aacagttgat
     7201 cgaatttttc ccgctcgggg gaaaccagac cgcaagagag tccgaagcga gtgttattcg
     7261 ccattagaat cgcttcctcg aaagaatcat aacgccagac gcgcaataac ggtccgaaca
     7321 cctcttcatc tggtacgcca gcaacgcctg tcatttcaat gatccccggc gtcagcaacg
     7381 atgtctctga ttgtaataag cgcggcgcaa gcagggttcg tccgcccatc gcttccagtt
     7441 gctgccaggc agtaaccacc tgctgtgcgg cctgttcaga aatcagcccg ccaataaacg
     7501 gctgcggttc gtcatcccag ttgcccggcg ttaatcgctg gctgacggca accagacgag
     7561 caagaaacgc atcgccctgc gctccgcttt tgagcaataa acggcgggcg caggtgcagc
     7621 gttgcccggc tgtgacaaac gccgactgaa tggtcagatg gacagccgcg tcgatatccg
     7681 ccacctcatc gataattagc ggattattac cgcccatctc aagggcgaga attttctccg
     7741 gctgaccgga gagctggcga tgcagctggt agccggtatt ggcgctaccg gtaaacagca
     7801 aaccgtcgag atcctccagc gcactcagcg cctgacccgt ttcacgcccg ccctgcacca
     7861 ggttcagtac gcccggcggc aagccagcct gctgccataa acgcattacc gcttcgccac
     7921 tccacggtgt cagttcgctg ggtttaaaga taatggtgtt acctgccagc aatgccggaa
     7981 cgatatgtcc gttcggcaaa tgaccaggga aattatacgg cccaaacacc gccagcacgc
     8041 cgtgcgggcg atgtcgcagg ctcgccgcac cgtccggcat ttcactacgc tgctcgccgg
     8101 tacgagcgtg atacgcctta attgatatcg cgattttatt gatcatcgcc gtcacttcgg
     8161 ttgccgcttc ccagcgcggc ttgccggttt ctcgggcaat gattgcggtt aattcgcctt
     8221 tattcctttc cagcaatccg gcaaagcgtt cgacaacgac ctgacgttca gccaatgaga
     8281 gccgcgccca gcgcggaaac gccgcacggg ctgcccgaca agcctgctcg acctgagcgg
     8341 catcggcatc attgccttgc cataacacct cgcccgatac cggattacgc ttcacacgcg
     8401 atgcgccctg gcccgttatc cagtcaccgt taatccataa agtcatgctg ttttctcctc
     8461 tgcgcacagg cgcaccagac gaacgcgatc ccctgcgtgg catttgaggg catccagttg
     8521 tgcggcggtt aaaatcaaac gctcggttgc cggatcggta cgcgccagca ccacgcggaa
     8581 atggtgataa ttttcattgg cgaccaggca ggccggaaaa tcgccctgcg caggctgccc
     8641 ttctgccact tccaccagcc gacttttacg aatggcgcgc acgcggtcga tgtcacactc
     8701 aagcgttggc ccgccgtcga agatgtcgat atagttgcgg taacgaaaac cttctttctc
     8761 cagcaccgcg cgggcaggcg cggtttgcgg atgtacctga ccgatgacgt cctgggcttc
     8821 ctgggataaa aagtgggtat agatcggatg tttcggcatc agttctgcaa taaatgcctt
     8881 ttgcccggtg ccgcagagaa aatcggcgcg gctaaaatcc atcgaaaaga agcgtttacc
     8941 gaggctttgc cagaacggtg aatagccgtg ttcgtcaatc accccgcgca tttcagcaac
     9001 cactttgtca ttaaacttgt cgcgaaaagc cgccataaac ataaagcgcg atttcgacag
     9061 caaatagccg ttgccctctt tgcgccagtc cgggtcgaga aacagcgtgc acagctcgct
     9121 gctgccggtg tgatcgttac tgagaaacag cgtcggcaat gcgttataga cattcagctc
     9181 ttttgaggcg tgaaccaacg tgccgacgcg atagttgtac cagggatcgt tcagcccaac
     9241 cgccacctca atggcacaaa tccccgccac ggtgcctgtc tcgctatctt ccagcacgaa
     9301 cacatagccc tgctcacttt tgggcagttc gccttgccag gttttgattg ccctttcgat
     9361 acgcgccgaa agcgtggctt cattggcggg aagcgacgtc aggccgccgc ccgttttgct
     9421 ggcaagctgc atcagcgccg agacatctga tcgctcaacg ggacggatga ccatcatgat
     9481 gaacctcggc taacaaagtg ttcgcaagca gctgcaaagc gatccagtcc ggtcgtcacc
     9541 tcttcttcgc tgacattgag cgcaggcgca aaacgcacca cgttgccacc cgcaatcagt
     9601 accatcacgc ttgctttcgc cgcttcctga gagatctgtt tcgcttgccc ggcgtaatcg
     9661 gcattcagta cacagccaat cagcaaacct aagccgcgaa cttcactgaa caaaccatag
     9721 cggtgattaa tagtattaag acgctcaaca aaccagtcgt gacgctgttt aacgccatta
     9781 agcatctctg gtgtgttgat gagctccagc actttgcctg ccaccgccga ggccagcgga
     9841 ttaccgccat aggtggtgcc atgagtgcca acggtcatca cgcgggcgca ctcttcggtt
     9901 gccaacaacg caccgaccgg gaaaccgccg cccagcgctt tggcggtagt taacagatca
     9961 ggcgtcacgc cgtagtgcat ataggcatac agttccccgg tgcgcccgac gccggtttgt
    10021 acttcatcaa aaatcaacag cgcattgtgg cggttacaca attcacgcag accttgtaaa
    10081 aacgcgttgc tggctggcac cacaccgcct tccccctgga tgggttcgac aatcaccgca
    10141 caggtagagt cgtcaatcag cgcgctggca gagttaatat cgttatatgc agcatgacga
    10201 atatccgccg gcagtggcgc aaaatcctgt gaataggctg gctgcccacc cgcactgaca
    10261 gtaaacagcg tgcgaccatg aaacgcattt ttgaacgcca cgatgccgct cttatggctg
    10321 ccgtagcggt cgtgagcgaa tttacgcgcc agttttagcg ccgcttcgtt ggcttccgca
    10381 ccggagttac aaaagaagac gcgatcggca aacgtggcgt cgatcaattt tttcgccagt
    10441 cgcagtaccg gctcgttggt gtaaccgttg ccggtatgcc agaacttact cgcctgttcg
    10501 ttcagcgctt cacgcagttc cggatgcgca tggcccagcg cgttcaccgc aatgccaccc
    10561 gcgaagtcga tatactcttt cccctgctga tcccacaagc gcgaaccttc gccacgtacc
    10621 ggtataaagg gtgccggagc gtaaacaggt atcatccatt catcaaagtt ttcacgcgta
    10681 attggctgag acatagcgac ctctatagta aataattcgt tatttatatg ttaacaataa
    10741 gtaatgtttg cgctgtaaat gtagattgca gggttcgtgc cagccagtga taaaagtgca
    10801 taaacggcgg aggctaactg gaaatcaagg agttataacc aaaccatatg catttaaagt
    10861 gcatataaag tgaatacgtt tgcgatgtgg gtgaataaaa agaataaaaa acgcaatgtt
    10921 atgcagaagt aaaatataat tctggaattg tgatcattga cgaaatttac tggaaattac
    10981 tgcgccattc tgacgcagcg cgcaccaaaa gcgggcattt tttgcgccat cgttgacatc
    11041 attaacaacc atcgatcaaa tcacttaaca acaggcggta agcaacgcga aattctgcta
    11101 ccatccacgc actctttatc tgaataaatg gcagcgacta tgaaatttgt ctcttttaat
    11161 atcaacggcc tgcgcgccag acctcaccag cttgaagcca tcgtcgaaaa gcaccaaccg
    11221 gatgtgattg gcctgcagga gacaaaagtt catgacgata tgtttccgct cgaagaggtg
    11281 gcgaagctcg gctacaacgt gttttatcac gggcagaaag gccattatgg cgtggcgctg
    11341 ctgaccaaag agacgccgat tgccgtgcgt cgcggctttc ccggtgacga cgaagaggcg
    11401 cagcggcgga ttattatggc ggaaatcccc tcactgctgg gtaatgtcac cgtgatcaac
    11461 ggttacttcc cgcagggtga aagccgcgac catccgataa aattcccggc aaaagcgcag
    11521 ttttatcaga atctgcaaaa ctacctggaa accgaactca aacgtgataa tccggtactg
    11581 attatgggcg atatgaatat cagccctaca gatctggata tcggcattgg cgaagaaaac
    11641 cgtaagcgct ggctgcgtac cggtaaatgc tctttcctgc cggaagagcg cgaatggatg
    11701 gacaggctga tgagctgggg gttggtcgat actttccgcc atgctaatcc gcaaacagca
    11761 gatcgtttct catggtttga ttaccgctca aaaggttttg acgataaccg tggtctgcgc
    11821 atcgacctgc tgctcgccag ccaaccgctg gcagaatgtt gcgtagaaac cggcatcgac
    11881 tatgaaattc gcagcatgga aaaaccgtcc gatcacgccc ccgtctgggc gaccttccgc
    11941 cgctaattta gcagctctcc tggctcaaac tgggtcagga gaattaacct tgagaaaaat
    12001 caacaaactg tcagtaatga tttgttgcct gccgtccttt gttataccgt cactgcgttt
    12061 ttagttgtct gaccacttct ctattatcaa gtttgatata ggaaactcca cgatgaacgc
    12121 tgagcgtaaa tttcttttgg cctgtcttat ttttgcgctg gtcatttacg ctatccacac
    12181 tttcggttta ttcgatctgc tcaccgattt accccactta cagacactca tccgccagag
    12241 cggattgttc ggttatagcc tctatattct gttattcatt attgccactc tctttctgtt
    12301 accaggaagc atactggtga tcgctggagg aatagttttt ggcccgctct tagggacact
    12361 actctcatta attgccgcca cgctcgcctc ctcgtgctca ttcctgctgg cgcgctggct
    12421 gggacgtgat ttactgctga aatacgttgg tcatagccat acctttcagg ccattgaaaa
    12481 aggcattgcg cgtaacggta ttgattttct tattctgacc cgcttaatcc cgttgtttcc
    12541 ttacaatatt caaaattatg cttacggatt aaccacaatc gccttctggc cttataccct
    12601 tatttcggca ctcacgaccc tgcccggtat tgttatttat accgtgatgg caagcgatct
    12661 cgccaatgaa ggcattacgc tgcgctttat tttacaactc tgtctggcgg gcctggcgct
    12721 gtttattctc gtccagctcg caaaactcta cgcccgacac aaacatgtgg atctgtctgc
    12781 ttcgcgccgc agcccactta ctcacccaaa aaatgaagga tagaacgatg tcgcaacatt
    12841 attcagtgtc atggaaaaaa ggactggctg cactctgctt actggctgtt gccgggctta
    12901 gcggctgcga tcaaaaagag aatgcggcgg caaaagtgga atacgacgga ctttcgaaca
    12961 gccaaccgct gcgcgtcgat gccaataacc atacggtaac catgctggtg caaattaatg
    13021 gtcgtttcct caccgacgac actcgtcacg gtattgtgtt taaagatggc tccaacggac
    13081 ataaatcgct gtttatggct tatgcgaccc cgaaagcatt ttatgaagcc ctgaaagagg
    13141 caggtggtac gccgggcgaa aacatgacga tggataataa agaaacgact catgtcacag
    13201 gcagcaaact ggatatttcg gtcaactggc aaggggcggc aaaagcgtat tccttcgatg
    13261 aagtgattgt tgacagtaat ggcaagaaac tggacatgcg ctttggcggt aatttaacgg
    13321 cagcagaaga gaagaaaacg ggttgactgg tgtgtctgga tagctgcccg gtcggcatcg
    13381 tcagcaatgc aacatacact tatggtgcgg tggaaaaacg cggcgaagtg aaattcaaag
    13441 gcaatgcctc agttctcccg gcggataaca cgctggcaac ggttaccttt aaaatcaccg
    13501 aataaagcca ggataaagga tgatgaagat acaatcgcgt aaaatctggt actaccgtat
    13561 aaccctcatc atcctgttgt tcgctatgct gctcgcatgg gcgctgcttc ccggcgtcca
    13621 tgagtttatc aatcgcagcg ttgcggcgtt tgccgccgtg gaccaacagg gtatagaacg
    13681 ctttattcag tcttacggcg cactagcggc agttgtctcg ttcttgttga tgattttaca
    13741 ggccattgct gcaccgctac ctgcgttttt gatcaccttt gccaatgcgt cgctgtttgg
    13801 cgcgttctgg ggcggcttgc tgtcgtggac cagttcgatg gccggcgcgg cgctgtgctt
    13861 ttttatcgcc agagtgatgg gccgcgaagt ggtggaaaaa ttaaccggca aaaccgtgct
    13921 tgacagtatg gacggctttt tcactcgcta cggcaaacac accatcctgg tctgtcggtt
    13981 attgcctttt gtccctttcg atccaatcag ctatgctgcc ggtctgactt caatacgttt
    14041 tcgctcgttt tttatcgcca ccgggcttgg tcaattaccg gcgactattg tttattcctg
    14101 ggcgggcagc atgttaacag gcggtacttt ctggtttgtc accggactgt ttattctgtt
    14161 tgccctgacc gtggtgattt ttatggcgaa gaaaatatgg cttgagcgcc agaagaggaa
    14221 tgcctgatgg gtttaccgcc gcttagcaaa attcctttca ttttacgtcc gcaggcgtgg
    14281 ctgcatcgtc gccattacgg cgaggtgcta agcccgattc gctggtgggg gcggatcccg
    14341 tttatctttt atttggtgtc aatgtttgtt gggtggctgg agcgcaaacg ctcaccgctc
    14401 gatccggtag tgcgatcgct tgtcagcgcg cgcattgcgc aaatgtgcct gtgtgagttt
    14461 tgcgtggata tcaccagtat gaaagtcgcc gagcgtaccg gcagcaccga taaactgctg
    14521 gcagtggctg actggcggca aagcccgctc ttcagcgatg aagaacggct ggcgctggag
    14581 tacgccgaag ccgcaagcgt aacgccgcca acggtcgatg atgccctgcg cacccgactg
    14641 gctgcgcatt ttgacgctca ggcgctcacc gaactgacgg cattgatcgg cctgcaaaac
    14701 ctgtcggctc gttttaattc tgccatggac attcccgctc aggggctgtg ccgtattccg
    14761 gaaaaacgtt cttaaggaga gaagatgcgc cattgtgggt ggttgctggg attgttatcg
    14821 ctgttttctc tggcaacaca tgccagtgac tggcaggaaa ttaaaaatga ggccaaaggg
    14881 caaaccgtct ggtttaacgc ctggggcggc gataccgcaa ttaaccgcta tctcgactgg
    14941 gttagcggcg agatgaaaac ccattacgct ataaacctga agattgttcg tctggcggat
    15001 gccgcagacg cggtgaagcg cattcagacc gaagctgctg ccggacgtaa aacgggcggc
    15061 tcggtggatc tgctctgggt gaacggcgaa aacttccgca ccttaaaaga ggccaattta
    15121 ttacaaacgg gctgggcgga gactctgccc aactggcgct atgtcgacac acagctgccg
    15181 gtgcgggaag atttttcagt gcctacagaa ggggctgaat cgccctgggg cggcgcacaa
    15241 ctgacgttta tcgcccgccg cgatgttacg ccacagccac cacaaacgcc gcaagcctta
    15301 ctggagtttg ctaaagccaa tcccggcacg gttacctatc cgcgcccacc ggactttacc
    15361 ggcacggcgt ttcttgaaca gttgctgatt atgctgacgc ccgatcccgc cgcgttaaaa
    15421 gttgccccgg ataaagccac tttcgcccgc gtcactgcgc ccttgtggca atatcttgac
    15481 gcgctacatc cctatttatg gcgcaaagga aaggatttcc cgccttcgcc cgcgcggatg
    15541 gatgctctgc tgaaagccgg cacattgcgc ctgtcgctga cctttaaccc tgctcatgcc
    15601 cagcaaaaaa tcgccagcgg cgatttgcct gcaagcagtt acagttttgg ttttaaccag
    15661 gggatgattg gcaacgtgca tttcgtcacc attcctgcta acgcaaatgc cagtgctgcg
    15721 gcgaaggtgg ttgccaactt cctgctctca cccaatgcgc aactgcgtaa agcggatccc
    15781 gctgtctggg gcgatccttc tgttctcgat ccgcaaaaac tgcctgatgg gcagcgcgaa
    15841 acattgcaat caagaatgcc gcaagatctg ccgccggtac tggctgaacc gcacgcaggt
    15901 tgggtaaatg cgctggaaca agaatggcta caccgttacg gtacgcatta atctttttgc
    15961 tgtgggcgat ggtggcggtg atttatgcac cactgatccc ggcagctctc acgctgatat
    16021 cgcctgcttt gtcgttgaca cactggcagg cgttatttgc cgatccacag ttaccgcaag
    16081 cattactggc aacgctggtg tcgacaacca tcgcggcggt cggggcgttg ttgattgccc
    16141 tgctggtgat tgtggcgctg tggccttggc cgaaatggca gcgtatgtgt gcccgtctgc
    16201 cgtggctgct cgccattccc catgtggctt ttgccaccag cgcccttctg ctctttgctg
    16261 acggagggct gctttatgac tatttcccgt atttcactcc gctaatggac aaattgggta
    16321 ttgggctggg cctcaccctt gcagtgaaag aaagcgcatt tctgctgtgg atcttagcgg
    16381 cagtattgag cgaaaaacgg ctgttgcagc aggtcattgt gctggattcg ctgggctaca
    16441 gccgctggca atgcctgaac tggctgctgt tgccctccgt cgcacctgca ctggcaatgg
    16501 cgatgttggc gattgttgcc tggtcgcttt cggtcgtgga tgtggcgatt attcttgggc
    16561 caggtaatcc accgacgctg gcagtaatta gctggcagtg gttaactcag ggcgacgcgg
    16621 atcaacaaac gaaaggcgca ctcgccagtc tgctgctgat gctgttactc gccgcctacg
    16681 ttttgctgag ctatctgcta tggcgcagct ggcggcgcac tattccccgc gtagatggca
    16741 ttcgcaagcc tgccacacct ttattaccag gcactacgct ggcgagtttt ttacccttaa
    16801 ccggtgtgct gtgtgtggtt ctgctggcga tcctcgcgga tcagtcgacg atcaatagtg
    16861 aagcactcat caacagcctg acaatggggc tggtggcgac attcatcgct ttgctcctgt
    16921 tactgctgtg gctggaatgg gggccacagc gtcgccagtt gtggctctgg ttgcccattc
    16981 tattgcccgc tctgccactg gtggcgggcc agtacacgct ggcgctatgg ctgaacctgg
    17041 atggaagctg gacggcggtg gtctgggggc atctgctgtg ggtaatgccg tggatgctgt
    17101 ttatcctgca acccgcctgg cagcgcattg attcacggtt aattttgatt gcgcaaacac
    17161 tgagctggtc gcgggccaaa atcttctttt acgtgaaatg tccgcttatg ctacgcccta
    17221 cgctgattgc cttcgcggtg ggattttcag tcagtattgc gcagtatatg ccgacgctgt
    17281 ggctgggcgc ggggcgtttt ccgacgctca ccactgaggc ggtggcatta agcagcggcg
    17341 gcagcaatgg tattttcgcc gcccaggctt tatggcaact gctattaccg cttattattt
    17401 ttgccctgac cgcgttagtc gcaaaatggg taggttatgt cagacaagga ctccgctaat
    17461 gctctgcgtg aaaaatgttt cgctacgttt accagaaagc cgcttgctga caaacgttaa
    17521 ctttacggtg gataaaggtg acattgtcac gttaatgggg ccgtctggct gtggaaaatc
    17581 cactctgttt tcatggatga ttggtgcact ggctggacag ttttcttgta caggtgagct
    17641 atggctcaat gagcaacgga ttgacatgct acccaccgca cagcgtcaga ttggcattct
    17701 tttccaggat gcactgttat ttgaccagtt cagcgtcggg caaaatttac tgctggcgct
    17761 accgtcgacg ctcaagggga ctgccaggcg caatgccgtg aaagacgcgc ttgatcgagc
    17821 gggactggct gaaacttatc accaggaccc tgcgacatta tcaggcggtc agcgagcgcg
    17881 ggtggccctg ctgcgcgccc ttctcgctca gccaaaagcg ttactcctgg atgagccatt
    17941 cagccgtctt gatgtggctc tgcgcgataa ttttcgccag tgggtgttca gcgaagttcg
    18001 cgaactggcg atccccgtcg ttcaggtaac gcacgatctc caggatgttc ctgctgatag
    18061 ttctgttctg gatatggcgc agtggtcaga aaattacaac aaactgcgat aacgcaaagt
    18121 ttttctcaat gcgtcagttc agaatggcgc actcaaaact acaatgtcgg gattttcgat
    18181 gaaacgtgtt tctcaaatga ccgcgctggc aatggcttta gggctggctt gcgcttcttc
    18241 gtgggccgct gaactggcga agcctcttac acctgaccag cttcaacaac aaaatggcaa
    18301 agcgatagat actcgcccca gcgcgtttta taacggctgg ccacaaacct taaatggccc
    18361 ttctggtcat gaacctgccg ccttaaacct ctctgccagc tggctcgaca aaatgagcac
    18421 cgaacagctc aacgcgtgga tcaagcaaca taacctgaaa gccgatgccc cggtggcgct
    18481 gtacggtaat gacaaagatg tcgacgccgt caaaacgcga ctgcaaaaag caggttttac
    18541 gcatatctcc attctgagtg acgcgctaag cgaaccttcc cgtctacaaa aactgccgca
    18601 ctttgagcag ctggtttatc cgcaatggct gcacgacctg caacaaggta aagaggttac
    18661 ggcgaaacct gccggtgact ggaaagtcat tgaagcggcc tggggcgctc ctaagcttta
    18721 ccttatcagc catattcccg gtgctgacta catcgatacc aacgaagtgg aaagcgaacc
    18781 gctgtggaac aaagtttctg atgaacaact aaaagcgatg ctggcaaaac acggcattcg
    18841 ccatgacacc acggtcattc tgtacgggcg tgacgtatac gctggagcgc gtgtggcgca
    18901 aattatgctc tacgcaggcg tgaaagatgt gcgtctgctg gatggcggct ggcaaacctg
    18961 gtccgacgcg ggactgcctg ttgagcgagg aacgccaccg aaagtgaaag cggaaccgga
    19021 tttcggcgtg aagatcccgg cacaaccgca gttgatgctc gatatggaac aggcccgtgg
    19081 actgctgcat cgccaggatg catcgctggt gagcattcgt tcgtggccag aatttatcgg
    19141 tacgaccagc ggttacagct atattaaacc aaaaggtgaa atagccggag cacgttgggg
    19201 acacgctggt agcgactcga cgcatatgga agattttcat aacccggatg gcaccatgcg
    19261 cagcgccgat gatattaccg ctatgtggaa agcgtggaat atcaaaccag agcagcaagt
    19321 ttcattctac tgcggcaccg gctggcgcgc gtccgaaacc tttatgtacg cacgcgccat
    19381 gggttggaag aatgtttccg tgtatgacgg cggctggtac gaatggagca gcgatccaaa
    19441 aaatccggta gcaaccggtg aacgcggccc ggacagtagc aaataacatt gcaattactg
    19501 acgctggtgc gacttcagcg tcagataccc actccacacc cgcgtaaagg tcgtcatcca
    19561 gcacaacgca ccgaagatcc acgcaaacca cgcaaaccat gcagggaata aacatcccag
    19621 cacaaacagt aagatcgttt ctgtgccttc ggttaacccg cccagataat aaaacgattt
    19681 atgcgcataa ccgggattat caatctgatg tttcgccgcc agcgcagcaa aagcgagaaa
    19741 actgctgcct gtgccgataa acgcaaacag cagccagccg cctgccaggg cgttttgctg
    19801 cggtgcggcg aggataaacc caaaaggtac cagcgcgtaa aaaagaaaat cgagagaaat
    19861 atcaagaaag ccgcccgcgt cggtaagccc tctgcgccgc gccagcgcac cgtccagacc
    19921 atcaagcagc ctgttcaaca aaatgacgac aagcgccgcc agataccagc ccagcgccag
    19981 aaaaggcagt gccagcacgc cgatggcaaa tcccactaat gttaaaccat ctggcgtaat
    20041 gcccggttta tcaagaaccc gcacgcactg atgcaacaac ggtttaatcc ggggatgaag
    20101 atggcggtct agcacaggca ctccttaaat ataaagcctt tctgattgag caacagtgcg
    20161 gatattatgg catttttcgc ttatctgccc gtgtgtaatt tatgaaaatg attgaagttg
    20221 ttgccgccat cattgaacgt gatggcaaaa ttttactcgc gcaacgcccc gcccagagcg
    20281 atcaggcggg attatgggag tttgccggtg gtaaagtcga gccggatgaa agccagcggc
    20341 aggcgctggt gcgtgagtta cgcgaagaac tgggcatcga agcaactgtg ggtgagtatg
    20401 ttaccagcca tcagcgagaa gtttcgggcc ggattatcca tcttcatgcc tggcacgtac
    20461 ccgacttcca cgggacgtta caggcacatg aacatcaggc gctggtctgg tgctcacctg
    20521 aagaggcgct gcaatatccg ctggcccctg ctgacattcc attattagag gcgtttatgg
    20581 ctttacgcgc cgccagacca gcggattagt gctaagggtt ttgtcatccc gctagcattg
    20641 cagcagtatt ccctcggctt taattaccgc gccttcagaa taatttttat cctgataaac
    20701 gcagcactga gtacagggct gcgctgactg cccgcctgaa ctgaacactt ctggcggtac
    20761 gtttacctcc acgtccggac gataatgcgg gttagccagt gcaattaatg gaaatgctaa
    20821 tactacggcg aacaatgctc gactcacagg gaactcctta acgttattgt ctctgctact
    20881 gataacggta gccgggtggc aaaactttag cgtctgagtt atcgcatttg gttatgagat
    20941 tactctcgtt attaatttgc tttcctgggt catttttttc ttgcttaccg tcacattctt
    21001 gatggtatag tcgaaaactg caaaagcaca tcacataaac aacataagca caatcgtatt
    21061 aatatataag ggttttatat ctatggatca gacatattct ctggagtcat tcctcaacca
    21121 tgtccaaaag cgcgacccga atcaaaccga gttcgcgcaa gccgttcgtg aagtaatgac
    21181 cacactctgg ccttttcttg aacaaaatcc aaaatatcgc cagatgtcat tactggagcg
    21241 tctggttgaa ccggagcgcg tgatccagtt tcgcgtggta tgggttgatg atcgcaatca
    21301 ggtacaggtc aaccgtgcat ggcgtgtgca gttcagctca gccatcggcc cgtacaaagg
    21361 cggtatgcgc ttccatccgt cagttaacct ctccattctc aaattcctcg gcttcgaaca
    21421 aaccttcaaa aatgccctga ctaccctgcc gatgggcggt ggtaaaggcg gtagcgattt
    21481 tgacccgaaa ggtaaaagcg aaggcgaagt gatgcgtttc tgccaggcgc tgatgaccga
    21541 gctttatcgt cacctgggcg cggataccga cgttccggca ggtgatatcg gcgttggtgg
    21601 tcgtgaagtc ggctttatgg cggggatgat gaaaaagctc tccaacaata ccgcctgcgt
    21661 cttcaccggt aagggccttt catttggcgg tagtcttatt cgcccggaag ctaccggcta
    21721 cggtctggtt tatttcacag aagcaatgtt aaaacgccac ggtatgggtt ttgaagggat
    21781 gcgcgtttcc gtttctggct ccggcaacgt cgcccagtac gctatcgaaa aagcgatgga
    21841 atttggtgct cgtgtgatca ctgcgtcaga ctccagcggc actgtagttg atgaaagcgg
    21901 attcacgaaa gagaaactgg cacgtcttat cgaaatcaaa gccagccgcg atggtcgagt
    21961 ggcagattac gccaaagaat ttggtctggt ctatctcgaa ggccaacagc cgtggtctgt
    22021 accggttgat atcgccctgc cttgcgccac ccagaacgaa ctggatgttg acgccgcgca
    22081 tcagcttatc gccaatggcg ttaaagccgt cgccgaaggg gcaaatatgc cgaccaccat
    22141 cgaagcgact gaactgttcc agcaggcagg cgtactgttt gcaccgggta aagcggctaa
    22201 tgctggcggt gtagcaacgt caggccttga aatggcgcaa aacgctgcgc gcctgggctg
    22261 gaaagccgag aaagttgacg cacgtttgca tcacatcatg ctggatatcc accatgcctg
    22321 tgttgagcat ggtggtgaag gtgagcaaac caactacgtg cagggcgcga acattgccgg
    22381 ttttgtgaag gttgccgatg cgatgctggc gcagggtgtg atttaagttg taaatgcctg
    22441 atggcgctac gcttatcagg cctacaaatg ggcacaattc attgcagtta cgctctaatg
    22501 taggccgggc aagcgcagcg cccccggcaa aatttcaggc gtttatgagt atttaacgga
    22561 tgatgctccc cagggaacat ttcttatggg ccaacggcat ttcttactgt agtgctccca
    22621 aaactgcttg tcgtaacgat aacacgcttc aagttcagca tccgttaact ttctgcgata
    22681 gcagcagata tgccagtaaa gaaatcccat tagactattt ttttgataat cttcttcgct
    22741 ttcgaacaac tcgtgcgcct ttcgagaagc aagcattata taatgccagg ccagttcttc
    22801 ttcaattgtc ccgttttgaa aagctgtgct tgatatcgag atcatccatg ataattccgc
    22861 cgcccatatt agcttcgccg aggatttacc ggagcgatta ttagcgcaat cagagatata
    22921 gtctgaggga aaaacagcaa atttattcaa caaggcgata acctgctctg gggcttcctc
    22981 catgtttgct ttaaaggtat tggctccatg gtcgccagaa agaaaatgct ccattaaggc
    23041 acaataactt tcgctatctt cgatacccca ttgatcctct aaagactcgc gtcttttact
    23101 tatgatatcg atcgagtcaa aaggaagcac atgatattgg aaggtatctt tgccaggttc
    23161 aggctttcgc ggccagaact ccagcgtttc agaccattgc ttatgataga atcgataagg
    23221 tgcgatcaat tgtagcgcct gtaacttctc gatactgagc ggctcaatac ctttagcctg
    23281 ataataatgc agttgttctt tttttgcttt aaaaccggcc cgaacaataa gccccatcat
    23341 aattaataga taaagaaaag agcatcccgc ggtaatcagg cctctttcat tcaaaccgtt
    23401 ggatgttatc gctgcgaaca caaacattac agcgacaaca catgttaaat aaaaccccca
    23461 cttacaaagc agcatggcct tattttcttt aatc
//
LOCUS       DAPHDW010000068        22754 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.068, whole genome shotgun sequence.
ACCESSION   DAPHDW010000068 DAPHDW010000000
VERSION     DAPHDW010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22754)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22754)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22754
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.068"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..454)
                     /locus_tag="RH057_002582"
     CDS             complement(<1..454)
                     /locus_tag="RH057_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV3128934.1"
                     /translation="MLAKDRTNLKIEEIRMHKHHEIHRVKPLMPALCRIRQGKKVINW
                     ETHSLTVDNNQIILFPCGYEFYIANYPEAGLYLAEMLYYPIDLIEKFQNLYAITDQIR
                     NTTGFCLPQNPELIYCWEQLKTSISRGFSTQIQEHLAMGVLLSLGAHHV"
     gene            complement(464..1015)
                     /gene="ybcL"
                     /locus_tag="RH057_002583"
     CDS             complement(464..1015)
                     /gene="ybcL"
                     /locus_tag="RH057_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HDV3128935.1"
                     /translation="MKKPIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPADAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKIWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEIIPVYEVK"
     gene            complement(1184..1516)
                     /gene="emrE"
                     /locus_tag="RH057_002584"
     CDS             complement(1184..1516)
                     /gene="emrE"
                     /locus_tag="RH057_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HDV3128936.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(1850..2164)
                     /gene="fliE"
                     /locus_tag="RH057_002585"
     CDS             complement(1850..2164)
                     /gene="fliE"
                     /locus_tag="RH057_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HDV3128937.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQDSLPQPTISFAGQLHAALDR
                     ISDTQTAARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            2379..4037
                     /gene="fliF"
                     /locus_tag="RH057_002586"
     CDS             2379..4037
                     /gene="fliF"
                     /locus_tag="RH057_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
                     /protein_id="HDV3128938.1"
                     /translation="MNATAAQTKSLEWLNRLRANPKIPLIVAGSAAVAVMVALILWAK
                     APDYRTLFSNLSDQDGGAIVSQLTQMNIPYRFSEASGAIEVPADKVHELRLRLAQQGL
                     PKGGAVGFELLDQEKFGISQFSEQVNYQRALEGELSRTIATIGPVKGARVHLAMPKPS
                     LFVREQKSPSASVTVNLLPGRALDEGQISAIVHLVSSAVAGLPPGNVTLVDQGGHLLT
                     QSNTSGRDLNDAQLKYASDVEGRIQRRIEAILSPIVGNGNIHAQVTAQLDFASKEQTE
                     EQYRPNGDESHAALRSRQLNESEQSGSGYPGGVPGALSNQPAPANNAPISTPPANQNN
                     RQQQASTTSNSGPRSTQRNETSNYEVDRTIRHTKMNVGDVQRLSVAVVVNYKTLPDGK
                     PLPLSNEQMKQIEDLTREAMGFSEKRGDSLNVVNSPFNSSDESGGELPFWQQQAFIDQ
                     LLAAGRWLLVLLVAWLLWRKAVRPQLTRRAEAMKAVQQQAQAREEVEDAVEVRLSKDE
                     QLQQRRANQRLGAEVMSQRIREMSDNDPRVVALVIRQWINNDHE"
     gene            4030..5025
                     /gene="fliG"
                     /locus_tag="RH057_002587"
     CDS             4030..5025
                     /gene="fliG"
                     /locus_tag="RH057_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HDV3128939.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLKRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLANRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            5018..5704
                     /gene="fliH"
                     /locus_tag="RH057_002588"
     CDS             5018..5704
                     /gene="fliH"
                     /locus_tag="RH057_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HDV3128940.1"
                     /translation="MSDNLPWKTWTPDDLAPPQAEFVPMVEPEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLARGLEQGLAEAKSQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            5704..7077
                     /gene="fliI"
                     /locus_tag="RH057_002589"
     CDS             5704..7077
                     /gene="fliI"
                     /locus_tag="RH057_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HDV3128941.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGTETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPSPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            7096..7539
                     /gene="fliJ"
                     /locus_tag="RH057_002590"
     CDS             7096..7539
                     /gene="fliJ"
                     /locus_tag="RH057_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
                     /protein_id="HDV3128942.1"
                     /translation="MAEHGALATLKDLAEKEVEDAARLLGEMRRGCQQAEEQLKMLID
                     YQNEYRNNLNSDMSAGMTSNRWINYQQFIQTLEKAITQHRQQLNQWTQKVDIALNSWR
                     EKKQRLQAWQTLQERQSTAALLAENRLDQKKMDEFAQRAAMRKPE"
     gene            7536..8663
                     /gene="fliK"
                     /locus_tag="RH057_002591"
     CDS             7536..8663
                     /gene="fliK"
                     /locus_tag="RH057_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HDV3128943.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLALLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSEILADAQQADLLIPVDETPPVINDEQSTSTPLTTAQ
                     TMTLAAVAGNNTAKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDVPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTAAASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMVSPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTVNHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            8768..9232
                     /gene="fliL"
                     /locus_tag="RH057_002592"
     CDS             8768..9232
                     /gene="fliL"
                     /locus_tag="RH057_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HDV3128944.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            9237..10241
                     /gene="fliM"
                     /locus_tag="RH057_002593"
     CDS             9237..10241
                     /gene="fliM"
                     /locus_tag="RH057_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HDV3128945.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASVSGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVKFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            10238..10651
                     /gene="fliN"
                     /locus_tag="RH057_002594"
     CDS             10238..10651
                     /gene="fliN"
                     /locus_tag="RH057_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HDV3128946.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSSKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            10654..11019
                     /gene="fliO"
                     /locus_tag="RH057_002595"
     CDS             10654..11019
                     /gene="fliO"
                     /locus_tag="RH057_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HDV3128947.1"
                     /translation="MNNHATVQSSAPVSAAPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRSGRS"
     gene            11019..11756
                     /gene="fliP"
                     /locus_tag="RH057_002596"
     CDS             11019..11756
                     /gene="fliP"
                     /locus_tag="RH057_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HDV3128948.1"
                     /translation="MRRLLSVAPVLLWLVTPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVSSLAQSFYS"
     gene            11766..12035
                     /gene="fliQ"
                     /locus_tag="RH057_002597"
     CDS             11766..12035
                     /gene="fliQ"
                     /locus_tag="RH057_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HDV3128949.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            12044..12829
                     /gene="fliR"
                     /locus_tag="RH057_002598"
     CDS             12044..12829
                     /gene="fliR"
                     /locus_tag="RH057_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HDV3128950.1"
                     /translation="MMQVTSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGVALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPASHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     GEPLNSNAFLALTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            13119..13742
                     /gene="rcsA"
                     /locus_tag="RH057_002599"
     CDS             13119..13742
                     /gene="rcsA"
                     /locus_tag="RH057_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HDV3128951.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(13786..13974)
                     /gene="dsrB"
                     /locus_tag="RH057_002600"
     CDS             complement(13786..13974)
                     /gene="dsrB"
                     /locus_tag="RH057_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HDV3128952.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVSLEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            14137..14364
                     /gene="yodD"
                     /locus_tag="RH057_002601"
     CDS             14137..14364
                     /gene="yodD"
                     /locus_tag="RH057_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416463.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HDV3128953.1"
                     /translation="MKTAKEYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            14662..15477
                     /locus_tag="RH057_002602"
     CDS             14662..15477
                     /locus_tag="RH057_002602"
                     /EC_number="3.1.3.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
                     /protein_id="HDV3128954.1"
                     /translation="MFSIQQPLLVFSDLDGTLLDSHSYDWQPAAPWLTRLREANVPVI
                     LCSSKTSAEMLYLQKTLGLQGLPLIAENGAVIQLAEQWQEIDGFPRIISGISHGEISQ
                     VLNTLREKEHFKFTTFDDVDDATIAEWTGLSRSQAALTQLHEASVTLIWRDSDERMAQ
                     FTARLNELGLQFMQGARFWHVLDASAGKDQAANWIIATYQQLSGKRPTTLGLGDGPND
                     APLLEVMDYAVIMKGLNREGVHLHDEDPARVWRTQREGPEGWREGLDHFFSAR"
     gene            complement(15474..17168)
                     /gene="yedQ"
                     /locus_tag="RH057_002603"
     CDS             complement(15474..17168)
                     /gene="yedQ"
                     /locus_tag="RH057_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
                     /protein_id="HDV3128955.1"
                     /translation="MQHETKMENQSWLKKLARRLGPGHVVNLCFIVVLLFSTLLTWRE
                     VVVLEDAYISSQRNHLENVANALDKHLQYNVDKLIFLRNGMREALVAPLDFTSLRNAV
                     TEFEQHRDEHAWQIELNRRRTLPVNGVSDALVSEGNLLSRENESLDNEITAALEVGYL
                     LRLAHNSSSMVEQAMYVSRAGFYVSTQPTLFTRNVPTRYYGYVTQPWFVGHSQRENRH
                     RAVRWFTSQPEHASNTEPQVTVSVPVDSENYWYGVLGMSIPVRTMQQFLRNAIDKNLD
                     GEYQLYDSKLRLLTSSNPDHPTGNIFDPRELALLAQAMEHDTRGGIRMNSRYVSWERL
                     DHFDGVLVRVHTLSEGVRGDFGSISIALTLLWALFTTMLLISWYVIRRMVSNMYVLQS
                     LLQWQAWHDTLTRLYNSGALFEKARSLAKLCQTHQHPFSVIQVDLDHFKAINDRFGHQ
                     AGDRVLSHAAGLISSSLRAQDVAGRVGGEEFCVILPGASLTQAAEVAERIRLKLNEKE
                     MLIAKSTTIRISASLGVSSSEETGDYDFEQLQSLADRRLYLAKQAGRNRVFASDNA"
     gene            complement(17339..17521)
                     /locus_tag="RH057_002604"
     CDS             complement(17339..17521)
                     /locus_tag="RH057_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HDV3128956.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETELVPGEGSRSAEEV"
     gene            complement(17600..18517)
                     /locus_tag="RH057_002605"
     CDS             complement(17600..18517)
                     /locus_tag="RH057_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HDV3128957.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHFAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            18690..19610
                     /gene="yedA"
                     /locus_tag="RH057_002606"
     CDS             18690..19610
                     /gene="yedA"
                     /locus_tag="RH057_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HDV3128958.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVESWPPLMMAGVRF
                     LAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     VATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(19599..20069)
                     /gene="vsr"
                     /locus_tag="RH057_002607"
     CDS             complement(19599..20069)
                     /gene="vsr"
                     /locus_tag="RH057_002607"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HDV3128959.1"
                     /translation="MADVHDKATRSKNMRAIATRDTAIEKRLASLLTGLGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGCRVLIVWECALRGREKLTDEALSERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(20050..21468)
                     /gene="dcm"
                     /locus_tag="RH057_002608"
     CDS             complement(20050..21468)
                     /gene="dcm"
                     /locus_tag="RH057_002608"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HDV3128960.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKTLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPAHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHKDGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AIFVLENVKNLKSHDQGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(21535..22230)
                     /locus_tag="RH057_002609"
     CDS             complement(21535..22230)
                     /locus_tag="RH057_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HDV3128961.1"
                     /translation="MDLQHWQTQFENWLKNHHQHQDAAHDVYHFRRVWATAQKLAADD
                     DVDMLVILTACYFHDIVSLAKNHPQRQRSSILAAEETRRLLREEFEQFPAEKIEAVCH
                     AIAAHSFSAQIAPLTTEAKIVQDADRLEALGAIGLARVFAVSGALGVALFDGEDPFAQ
                     HRPLDDKRYALDHFQTKLLKLPQTMQTARGKQLAQHNAQFLVEFMAKLSAELAGENEG
                     VDHKVIDAFSPAG"
     gene            complement(22270..22627)
                     /locus_tag="RH057_002610"
                     /pseudo
     CDS             complement(22270..22627)
                     /locus_tag="RH057_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
BASE COUNT         5589 a         5975 c         5765 g         5425 t
ORIGIN      
        1 taacatgatg tgctcctaat gaaagtagaa cgcccattgc taagtgttcc tgaatttgcg
       61 ttgagaagcc tcgggaaata gatgttttta gttgctccca acaatatatt aactcgggat
      121 tctgaggtaa gcagaaacct gtcgtgttac gaatttgatc ggttatcgca taaagatttt
      181 ggaacttttc aattagatca atgggataat aaagcatttc tgcaagataa agacctgctt
      241 ctgggtaatt cgcaatataa aattcatatc cacagggaaa taatattatc tgattattat
      301 caacagttag actatgcgtc tcccaattga taactttttt tccctgacgg atacgacata
      361 aagctggcat aagaggctta accctgtgaa tctcatgatg tttatgcatc cgtatttctt
      421 caatctttag gttagtccta tcttttgcca gcatactctt atcctacttt acctcataaa
      481 cgggtattat ctcagcggtt gcaattttat tggcattaag catataacca actaacgctc
      541 cgctggaatt agaatccaca ggaatcttat cagtttttag agcccatatt ttaaactggt
      601 aatgatgggg tttatctcct ttgggaggac atgctccacc aaatcccgca tagccaaaat
      661 catttcggcc ttggacagca ccagtcggaa gttttgtacc atcacgtctt cctgcatcag
      721 cgggcaaata tgttaccgtt gctggaatat tagcaacagt ccagtgccac catccgctgc
      781 ctgtaggcgc atctggatca tacacagtta cggcaaagct tttggtgcct tctggagcac
      841 cagaccaggt taatgagggc gatgtattac ccccctcaca cccaaatcca gaaaatacat
      901 gagacgttgt aagttgctct cctgttttta tttcattact agtgacctga aatgctgcgg
      961 cctgcgcaga aaatgttatg aatgccaata cacttgaaac gataggtttt ttcataaaaa
     1021 cctctttgtt gtgacctatc gttattttat ttgatgtgca ttgatctcat tatgcaaaaa
     1081 ggcgcaatga tcgcgcaaat gcaatcataa ttataaagtt gccccgaaca ttagccataa
     1141 tatgcacaaa tcagattcct gttatctcaa ggatgattat tttttaatgc ggtgcgcttc
     1201 gtgacaacaa gttaatgact aacacaccgg cacaaatcaa catcatgcct atgatggctg
     1261 gcaagtccag tcgttggcca aaaaatcccc aggaaagcaa actaatcaga acgataccga
     1321 ctcctgacca gatagcataa gcaatccctg taggtatata agccagtgtc tgagctaata
     1381 accaaaatga tgcacaatag caaataattg tgccaacaga gggccataac cgtgtaaacc
     1441 cctctgaaaa cttcattaag gttgtaccaa tgacctctgc aagtattgcg ccaccaagat
     1501 aaatataagg gttcatagca tattctttcc tgttcatact ggagagaatt gtactacagt
     1561 ttgaacacaa ctcaccgttt tcatcttggt gtatgtgtat ccagggggct ctggcgtggc
     1621 aagagataaa ggcaggaaca ataatgatca gaaggaaccc caggctaaca aaagagttca
     1681 ctcttcctat gagcaaagcg aagacaaaac atgttatcgc gtttacggat cgtggatggc
     1741 agaaaacttt caggatcagg tatccatttt aaaccagaaa ttgagtgagt ttgccccatc
     1801 catgccccaa gcggtgagat tggaagtaat taacaacagg ttacaaaacc tacacctgca
     1861 tggacatcac ttcctgatac gccgccacca gcttattacg cacctgaatc cccatttgca
     1921 tagaaactga ggctttttgc atatcggtca tcacatcgtt taacgccacg ccgggttcac
     1981 cgagggtgaa tttttctgcc tgcgtgcggg cagctgtttg tgtatcgcta atgcgatcga
     2041 gcgcggcgtg cagctgcccg gcaaaactaa tggtcggttg cggcagtgaa tcctgcgcac
     2101 gcgcactcat cgccgtagcc tgtaactggc tgataacccc ttcaatcccc tgtatcgctg
     2161 acattttcgt ctcctggata atttctggta gcaaagccta ccagtaagtc aataagacaa
     2221 aggcgctaaa tagcaacaaa aaaacgggtt tattggcgga tagaaaaaaa cgaaagcaca
     2281 aataattgca gcgtcaattt ttcgagtttg ctgacccggg agtgagtctt gttccacttt
     2341 gccaataacg ccgtccataa tcagccacga ggtgcgcgat gaatgcgact gcagcccaga
     2401 caaaatctct tgagtggctt aatcgcctgc gtgcgaatcc gaaaattcca ttgattgttg
     2461 ccggttccgc ggcagtggcg gtcatggtcg cactgatcct gtgggcgaaa gcccccgact
     2521 accgcacatt attcagcaat ctttccgatc aggatggtgg cgcaattgtc agccaactga
     2581 cgcaaatgaa tattccttac cgcttcagcg aagccagcgg cgctattgaa gttccggcag
     2641 ataaagttca cgaactgcgt ctgcgcctgg cacaacaagg tttgcccaaa ggcggcgcgg
     2701 tcggtttcga actactcgat caggaaaagt ttggtatcag ccagttcagc gaacaggtga
     2761 attatcagcg ggcgctggaa ggcgagcttt ctcgtaccat cgcaactatc ggcccggtaa
     2821 aaggggcgcg cgtacatctg gcaatgccga aaccgtcttt attcgtccgt gaacaaaaat
     2881 ccccttctgc atcggtgacg gtaaatctgt tacccggccg cgcactcgat gaagggcaaa
     2941 ttagcgccat tgtgcatctg gtttccagcg ccgttgctgg tctgccgccg ggaaacgtca
     3001 cgctggtgga tcagggcgga catctgttaa cccagtccaa taccagcggg cgcgatctta
     3061 atgatgctca gttgaaatat gccagcgatg tcgaaggccg tattcagcgg cgtattgaag
     3121 caatcctgtc gcctattgtt ggtaacggta atattcacgc ccaggtcacg gcgcagctgg
     3181 acttcgccag taaagaacaa acggaagaac agtatcgccc taacggtgat gaatctcatg
     3241 cggcgcttcg ttcacgccag cttaatgaga gcgagcaaag cggttccggt tatccgggcg
     3301 gcgtaccggg ggcgttgtcg aatcaaccgg cacctgcgaa taacgcgcca atcagcacgc
     3361 ctccggcaaa tcaaaataac cgccagcagc aggcgagcac caccagcaat agcgggccgc
     3421 gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca
     3481 aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct
     3541 tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt gaagatctga
     3601 cccgcgaggc gatgggcttt tctgaaaaac gcggtgactc gctcaatgtc gttaactcgc
     3661 cgttcaatag cagtgacgaa agcggcggag aactgccgtt ctggcaacag caagcgttta
     3721 tcgatcagtt gctggctgcc ggtcgctggt tgctggtact gctggtggcg tggctgctgt
     3781 ggcggaaagc ggtacgcccg cagttaacac gtcgcgccga ggcgatgaaa gctgtacagc
     3841 aacaggcgca ggcccgtgag gaagtggaag atgcggtgga agtccgcctg agcaaagacg
     3901 aacaactcca acaacgacgc gctaaccaac gtctgggggc agaagtcatg agccagcgta
     3961 tccgtgaaat gtctgataac gatccgcgcg tggtggcgct ggtcattcgc cagtggataa
     4021 ataacgatca tgagtaacct gacaggcacc gataaaagcg tcatcctgct gatgaccatt
     4081 ggcgaagacc gggcggcaga ggtgttcaag cacctctccc agcgcgaagt gcaaaccctg
     4141 agcgctgcaa tggcgaacgt cacgcagatc tccaacaagc agctaaccga tgtgctggcg
     4201 gagtttgagc aagaagctga acagtttgcc gcactgaata tcaacgccaa cgattatctg
     4261 cgttcggtat tggtcaaagc tctgggtgag gaacgtgccg ccagcctgct ggaagatatt
     4321 ctcgaaactc gcgataccgc cagcggtatt gaaacgctca actttatgga gccgcagagt
     4381 gccgccgatc tgattcgcga tgagcatccg caaattatcg ccaccattct ggtgcatctg
     4441 aagcgcgcgc aggccgccga tattctggcg ttgttcgatg aacgtctgcg ccacgacgtg
     4501 atgttgcgta tcgccacctt tggcggcgtg cagccagccg cgctggcgga actgaccgaa
     4561 gtactgaatg gcttgctcga cggtcagaat ctcaagcgca gcaaaatggg cggcgtgaga
     4621 acggcagccg aaattatcaa cctgatgaaa actcagcagg aagaagccgt tattaccgcc
     4681 gtgcgcgaat tcgacggcga gctggcgcaa aaaattatcg acgagatgtt cctgttcgaa
     4741 aatctggtgg atgtcgacga tcgcagcatt cagcgtctgt tgcaggaagt ggattccgaa
     4801 tcgctgttga tcgcgctgaa aggggccgag cagccactgc gcgagaaatt cctgcgcaat
     4861 atgtcgcagc gtgccgccga tattctgcgc gacgatctcg ccaaccgtgg tccggtgcgt
     4921 ctgtcgcagg tggaaaacga acagaaagcg attctgctga ttgtgcgccg ccttgccgaa
     4981 actggcgaga tggtaattgg cagcggcgag gatacctatg tctgataatc tgccgtggaa
     5041 aacctggacg ccggacgatc tcgcgccacc acaggcagag tttgtgccca tggtcgagcc
     5101 ggaagaaacc atcattgaag aggccgaacc cagccttgag cagcaactgg cgcaactgca
     5161 aatgcaggcc catgagcaag gttatcaggc ggggattgcc gaaggtcgcc agcaaggtca
     5221 tgagcagggc tatcaggaag gactggcccg ggggctggag caaggtctgg cagaggcgaa
     5281 gtctcaacaa gcgccaattc atgcccggat gcagcaactg gtcagcgaat ttcaaactac
     5341 ccttgatgcg cttgatagtg tgattgcttc gcgcctgatg cagatggcgc tggaggcggc
     5401 acgtcaggtg atcggtcaga cgccaacagt ggataactcg gcactgatca aacagatcca
     5461 acagttgttg cagcaagaac cgttattcag cggtaaacca cagctgcgcg tgcacccgga
     5521 tgatctgcaa cgtgtggatg atatgctcgg cgcaacctta agtttgcatg gctggcgctt
     5581 gcggggcgat cccacactcc atcctggcgg ctgtaaagtc tccgccgatg aaggcgatct
     5641 cgacgccagt gtcgccactc gctggcaaga actctgccgt ctggcagcac caggagtggt
     5701 gtaatgacca cgcgcctgac tcgctggcta accacgctgg ataactttga agccaaaatg
     5761 gcgcagttgc ctgcggtacg tcgctatggg cgattaaccc gcgctaccgg gctggtgctg
     5821 gaagccaccg gattacaatt gccgctcggc gcaacctgtg tcattgaacg ccagaacggc
     5881 actgaaacgc acgaagtaga aagcgaagtc gttggcttta acggtcaacg gctgttttta
     5941 atgccgctgg aggaagtcga aggtgtcctg cccggcgcgc gggtttatgc caaaaacatt
     6001 tcggctgaag gactgcaaag cggcaagcag ttgccgctcg gtccggcgtt attaggtcgc
     6061 gttctggacg gcagcggtaa gccgctcgat ggcctgccct cccccgatac gacggaaacc
     6121 ggtgcgctga ttaccccgcc atttaacccg ttgcaacgta caccgattga acatgtgctg
     6181 gacaccggcg tgcgcccaat caatgccctg cttaccgttg ggcgtgggca gcgtatgggg
     6241 ctgtttgccg ggtccggcgt tggtaaaagt gtgctgctgg ggatgatggc acgttacacc
     6301 cgcgccgatg tcattgtcgt gggtttgatt ggtgaacgtg ggcgcgaagt aaaagatttt
     6361 attgagaata tcctcggtgc cgaagggcgt gcacgctccg tggtgattgc cgctccggcg
     6421 gatgtctctc cgcttctgcg aatgcaaggt gccgcctatg ccacgcgcat tgccgaagat
     6481 tttcgcgatc gtggtcagca tgtgttgctg attatggact ccctcacccg ctacgcgatg
     6541 gcccagcgtg aaattgcgct ggcgattggc gaaccacccg ccaccaaagg ttatcccccg
     6601 tcggtgtttg ccaaattacc ggcactggtc gagcgtgccg gaaatggcat tagcggcggc
     6661 ggatcgatta ccgcgtttta taccgtgctc accgagggcg atgaccagca ggaccccatt
     6721 gccgactccg cgcgggcaat cctcgacggt cacattgtgc tgtctcgccg actggcggaa
     6781 gccgggcact atccggctat cgatattgaa gcgtcgatca gccgcgcaat gacggcgttg
     6841 atcagtgagc aacattacgc gcgagtgcgc accttcaaac agctgttgtc gagttttcag
     6901 cgtaaccgcg atctggttag cgtcggcgcg tatgccaaag gcagcgatcc gatgctcgac
     6961 aaagccatcg ccctgtggcc gcagctggag ggctatttgc aacaaggcat ttttgaacgc
     7021 gcggactggg aagcgtctct ccaggggctg gagcgtattt tcccgacagt gtcataaccc
     7081 aggagataac ggcagatggc agaacatggc gcgctggcga ccctgaaaga tctggcagaa
     7141 aaagaggtag aggatgccgc gcgcctgctg ggtgaaatgc gtcgcggatg tcagcaggcg
     7201 gaagaacagc tcaaaatgct gatcgattat cagaatgaat atcgcaataa cctcaatagc
     7261 gatatgagtg ccgggatgac cagcaaccgc tggatcaact atcagcagtt tatccagacg
     7321 ttggaaaaag ccattactca gcatcgccag caacttaatc agtggacgca gaaagttgac
     7381 attgccctga acagctggcg agaaaagaaa caacgtttgc aggcctggca gacactgcag
     7441 gaacggcaat ccacggcggc actgcttgca gaaaaccgcc tcgatcagaa aaagatggat
     7501 gagttcgccc agcgcgccgc catgaggaaa cctgaatgat tcgcttagcg cccttaatta
     7561 ccgccgacgt tgacaccacc acattgcctg gcggcaaagc cagcgatgct gcacaagatt
     7621 ttctcgcgtt gttgagcgaa gcattagcag gcgagacaac taccgacaaa gcggcccccc
     7681 agttgctggt ggcaacagat aagcccacga caaaaggcga gccgctggtc agcgagattc
     7741 ttgccgatgc gcaacaagcg gatttactga tccctgtgga tgaaacaccg cctgtcatca
     7801 acgacgaaca atccacatca acaccattaa ccaccgctca aacgatgacg ttggctgcgg
     7861 tggctggcaa caatacggca aaagacgaaa aagcggatga tctgaatgaa gacgtcaccg
     7921 caagcctgag cgcccttttt gcgatgttgc cgggttttga caatacgccc aaagtgactg
     7981 atgtgccgtc aaccgtgtta ccggcagaga aaccaacgct cttcacaaaa ctgacttctg
     8041 cgcaactcac aacagcacag cctgatgatg cccccggcac gccagctcag ccattaacac
     8101 cgctggtagc agaagcccag agtaaagcgg aagtcatcag cacaccttca ccggtgaccg
     8161 ctgccgccag cccgctaatt actccacacc agacacagcc actgcccacc gtcgccgcgc
     8221 ctgttttgag tgcaccgctg ggttctcacg aatggcaaca atcattaagc cagcatattt
     8281 cgctgttcac ccgccagggg caacaaagtg cagagttgcg tctgcacccg caggatttag
     8341 gtgaagtgca aatctccctc aaagtggatg ataaccaggc gcaaatccag atggtttcac
     8401 cgcatcagca tgtacgcgcc gcgctggaag cagcgcttcc ggtactgcgt acgcagctgg
     8461 ccgaaagtgg cattcagtta gggcaaagca acatcagtgg cgaaagcttt agtggtcagc
     8521 agcaggccgc ttcccaacaa cagcaaagcc aacgcacagt aaaccatgaa cctctggcgg
     8581 gggaagacga cgatacgctt ccggttcccg tctctttaca agggcgtgta acaggcaaca
     8641 gcggcgttga tattttcgcc taacgtcaga ggtagcaccg taatccgcgt cttttccccg
     8701 ctttgttgcg ctcaagacgc aggataatta gccgataagc agtagcgaca caggaagacc
     8761 gcaacacatg actgattacg cgataagcaa gaaaagcaag cgatcgcttt ggatcccgat
     8821 tctggtattc attaccctcg cggcctgtgc cagcgcaggt tacagctact ggcattcgca
     8881 tcaggttgcc gctgacgaca aagcgcagca acgcgtcgta ccctccccgg tcttctacgc
     8941 gctggatacc ttcacggtca atttgggcga tgcggatcgc gtgctttata tcggcataac
     9001 cctgcgcctg aaagatgaag ctacccgctc gcggctgagt gagtatttgc cggaagtccg
     9061 tagccgtttg ctgttactgt tttcgcgtca ggatgctgcc gtactggcga cagaagaagg
     9121 caagaaaaac ctgattgccg agattaaaac cacgctttcc accccgcttg ttgccgggca
     9181 accgaaacag gatgtcaccg acgtgctgta taccgctttt attctgcgat aacgacatgg
     9241 gcgatagtat tctttctcaa gctgaaattg atgcgctgtt gaatggtgac agcgaagtca
     9301 aagacgaacc gacagccagt gttagcggcg aaagtgacat tcgtccgtac gatccgaata
     9361 cccaacgacg ggttgtgcgc gaacgtttgc aggcgctgga aatcattaat gagcgctttg
     9421 cccgccattt tcgtatgggg ctgttcaacc tgctgcgtcg tagcccggat ataaccgtcg
     9481 gggccatccg cattcagccg taccatgaat ttgcccgcaa cctgccggtg ccgaccaacc
     9541 tgaaccttat ccatctgaaa ccgctgcgcg gcacagggct ggtggtgttt tcaccgagtc
     9601 tggtgtttat cgccgtggat aacctgtttg gcggcgatgg acgcttcccg accaaagtgg
     9661 aaggtcgcga gtttacccat accgaacagc gcgtcatcaa ccgcatgtta aagctggcgc
     9721 ttgaaggcta tagcgacgcc tggaaggcga ttaatccgct ggaagttgag tacgtgcgtt
     9781 cggaaatgca ggtgaaattt accaatatca ccacctcgcc gaacgacatt gtggttaaca
     9841 caccgttcca tgtggagatt ggcaacctga ccggcgaatt taatatctgt ctgccattca
     9901 gcatgatcga accgctacgg gaattgttgg ttaacccgcc gctggaaaac tcgcgtaatg
     9961 aagatcagaa ctggcgcgat aacctggtgc gccaggtgca gcattcacag ctggagctgg
    10021 tcgccaactt tgccgatatc tcgctacgcc tgtcgcagat tttaaaactg aaacccggcg
    10081 acgtcctgcc gatagaaaaa cccgatcgca tcatcgccca tgttgacggc gtcccggtgc
    10141 tgaccagtca gtacggcact ctcaacggtc agtatgcgtt acggatagaa catttgatta
    10201 acccgatttt aaattctctg aacgaggaac agcccaaatg agtgacatga ataatccggc
    10261 cgatgacaac aacggcgcaa tggacgatct gtgggctgaa gcgttgagcg aacaaaaatc
    10321 aaccagcagc aaaagcgctg ccgacgcggt gttccagcaa tttggcggtg gtgatgtcag
    10381 cggaacgttg caggatatcg acctgataat ggatattccg gtcaagctga ccgtcgagct
    10441 gggccgtacg cggatgacca tcaaagagct gttgcgtctg acgcaagggt ccgtcgtggc
    10501 gctggacggt ctggcgggcg aaccactgga tattctgatc aacggttatt taatcgccca
    10561 gggcgaagtg gtggtcgttg ccgataaata tggcgtgcgg atcaccgata tcattactcc
    10621 gtctgagcga atgcgccgcc tgagccgtta gtgatgaata accacgctac tgtgcaatct
    10681 tccgcgccgg tttctgctgc gccactgctg caggtgagcg gcgcactcat cgccattatt
    10741 gccctgatcc tcgctgctgc ctggctggta aaacggctgg gatttgcccc gaaacgcact
    10801 ggcgttaatg gtctgaaaat tagcgccagt gcttcgctgg gcgcgcgtga acgggttgtg
    10861 gtggtcgatg tggaagatgc acggctggtg ctcggcgtta ccgcagggca aatcaatctg
    10921 ctgcataaac ttcccccttc agcaccaacg gaagagatac ctcagaccga ttttcagtcg
    10981 gtcatgaaaa acttgcttaa gcgtagcggg agatcctgat gcgtcgtttg ttgtctgtcg
    11041 cccctgtcct tctctggctg gttacgcccc tcgccttcgc gcaactgcca ggtatcacca
    11101 gccagccgct gcctggcggt ggacaaagct ggtcgctccc ggtgcagacg ctggtgttca
    11161 tcacctcgtt gacgtttatt ccggcaattt tactaatgat gaccagtttc acccgcatca
    11221 ttattgtttt tggtttattg cgtaacgcgc tgggaacgcc ctccgcgcca cctaaccagg
    11281 tattgctggg actggcgctg tttttgacct tttttattat gtcgccggtg atcgataaaa
    11341 tttatgtcga tgcgtaccag ccattcagcg aagagaaaat atcaatgcag gaggcgctgg
    11401 aaaaaggggc gcagccgctg cgtgagttta tgctgcgtca gacccgtgag gcggatttag
    11461 ggttgtttgc cagactggcg aataccggcc cgttgcaggg acctgaagcc gtgccgatgc
    11521 gcattttgct cccggcctac gtgaccagcg agttgaaaac cgcatttcag ataggcttca
    11581 cgattttcat cccttttttg attatcgacc tggtgattgc cagcgtgttg atggcattgg
    11641 ggatgatgat ggtccccccc gccaccattg ctctgccctt taaactgatg ctgtttgtac
    11701 tggtggatgg ttggcaattg ctggtcagtt cgctggcgca gagcttttac agctagagag
    11761 gcaaaatgac acctgaatcg gtcatgatga tggggactga agcgatgaaa gtcgcgctgg
    11821 cactagctgc cccgctgttg ctggtggcgc tggtcacggg ccttatcatc agtattttgc
    11881 aggccgccac acagatcaac gaaatgacgc tgtcgtttat accaaaaatc atcgccgtat
    11941 ttattgccat tattattgcc ggaccgtgga tgctcaatct gttgctggat tacgtccgca
    12001 ccttgttcac taacctgccg tatatcatcg ggtagcccgt acaatgatgc aggtgacaag
    12061 cgatcaatgg ctttcctggt taagcctcta cttctggccg ttactgcgcg tgctggcgct
    12121 catttctacc gcgccaattc tgagcgaacg cagcgtgccg aaacgggtaa aactgggtct
    12181 ggcaatgatg atcacgttcg ctattgcccc atcattacct gccaacgatg ttcctgtttt
    12241 ttcgttcttt gctctgtggc tggccgtgca gcagatcctg atcggcgttg cgcttggttt
    12301 taccatgcaa tttgcctttg ccgctgtgcg aaccgctggc gaaattatcg gtctgcaaat
    12361 ggggctgtca tttgcgacgt ttgtcgatcc ggccagccat ctcaatatgc ccgttttagc
    12421 gcgtatcatg gatatgctgg cgttactgct gttcctgaca tttaacggtc atttatggtt
    12481 gatttcactg ctggtcgata cctttcacac cctgccaatt ggtggcgaac cgttgaacag
    12541 caatgcgttt ctggcactca ccaaagcggg gagtttgatt ttccttaacg ggctgatgct
    12601 ggcgttaccg ctcattactc tgctgctgac cctgaatctg gcattaggtt tactgaatcg
    12661 tatggccccg caattatcca tttttgttat tggatttcca ttaactctga ctgtcggtat
    12721 ctctttaatg gcggcattaa tgccgttaat tgcgcctttt tgcgaacatt tattcagtga
    12781 aatttttaat ttgctggctg atattattag tgaattgcca ttaatataat tccgtaacgt
    12841 ttatcatgtt atcctaagga ttatccgaaa aataatacct gcgaacatct tccaggatac
    12901 tcctgtggcg aaatatttgt tttaagctca ctcacatatc gcaacattta ctttacttta
    12961 agacaattcc aggcaaatta tacaacactt tacgggatag taagtccgcc tgaaaaatcg
    13021 cgagagtggc gcattaggtg acccatgttg ttccgtttag tcatgatgaa atattcaggt
    13081 aaggggaatt atcgttacgc attgagtgag ggtatgccat gtcaacgatt attatggatt
    13141 tatgtagtta cacccgacta ggtttaaccg ggtatctgtt gagtagaggg gttaaaaaaa
    13201 gagaaatcaa cgacattgaa accgttgatg accttgccat agcttgtgat tcacagcgcc
    13261 cttcagtggt gtttattaat gaggactgtt tcatccatga tgcttctaac agtcagcgca
    13321 tcaagcacat cattaatcaa catcccaata cgttatttat cgtttttatg gcaattgcca
    13381 atgttcattt tgatgaatat ctattggtca gaaaaaattt attgatcagt tctaaatcga
    13441 ttaaaccgga atctctcgac gatatccttg gcgatattct gaaaaaagag acaacgataa
    13501 cctctttttt aaatatgccg acgttatcat tgagccgaac cgaatcgagt atgttgcgaa
    13561 tgtggatggc aggtcaggga accattcaaa tctctgacca aatgaatatc aaagccaaga
    13621 ccgtttcatc gcataaaggt aatattaaac gtaagatcaa aacgcataat aaacaggtta
    13681 tctaccatgt cgtccgactg acggataatg tgactaatgg tatttttgtc aacatgcgct
    13741 aacacattct gactggtggt ttcccaccag tcaggctgaa taagattact ctgctttctc
    13801 cacaaagata ccgtcctgat gccctgcttc attaaagaac cagatgccga gcgggtagtc
    13861 ttccagcgaa accaggtaca ttgtgccttc actaaactcc tcaactgcca gtaccacgcc
    13921 aggacgacgc ggaccgccat ccgttttgac tgttacccga tcattcacct tcatcgtttt
    13981 cctcctgtgg ctttgtgcca gtgtagaaca atttcgtttt tctggcagcg ccaggcgcgc
    14041 gcgagtgctg attttctcga cggtctatac ttaagagatg ccaggcggac ttaacgactg
    14101 gcggcaacaa cagagtaacg gttgcgagga aagatgatga aaaccgcaaa agagtacagc
    14161 gataccgcaa aacgtgaggt cagcgtcgat gtcgatgccc tgctggcggc gatcaatgaa
    14221 attagcgaaa gcgaagttca tcgcagccag aacgattctg aacacgttag cgtcgatgga
    14281 cgtgaatatc atacatggcg tgaattggcg gatgccttcg aactggatat tcatgacttc
    14341 agcgtctctg aagtgaatcg ttgaatgcac aataaaaaaa tcccgaccct gagggggtcg
    14401 ggatgaaact tgcttaagca agaagcactt aaaaaattcg ttacaccagg aaatctgatg
    14461 tgttcatcac cttatccgca atttttttcg ctgacaagaa aatattcgcc atatgaatga
    14521 ttaacgcttc ttatgaatag aaatgtgacg cacgtcaccc cttggtggct ctggcgtcag
    14581 cgggatcgct cccgaaaaaa tcatctttga taatttgtgc ggttttgaga atcatcctgg
    14641 taagaggagt actgcgcgac tatgttttca attcaacaac cactactggt ttttagcgat
    14701 cttgatggca ccctgctgga cagtcatagt tatgactggc aaccggcagc cccctggctc
    14761 acccgtttac gcgaagcaaa tgttcccgtc attctctgta gcagtaaaac atcagcggaa
    14821 atgctgtact tgcaaaaaac gttggggcta caaggtttac cgctgattgc cgaaaatggc
    14881 gcagtgatcc agcttgctga gcaatggcag gagatagacg gttttccacg catcatctca
    14941 ggtattagcc atggcgaaat cagccaggtt ttaaatacgc tacgcgagaa agaacatttt
    15001 aaattcacga cttttgatga tgtcgacgat gcaaccatcg ccgaatggac gggattaagc
    15061 cgtagccagg cggcgctgac gcagctacat gaggcgtcgg taacgctaat ctggcgcgac
    15121 agtgacgagc gtatggcaca atttaccgct cgtctgaacg aactgggctt gcagtttatg
    15181 cagggtgcgc gcttctggca cgttctggat gcctctgccg gaaaagatca ggctgccaac
    15241 tggattatcg cgacctatca acaattgtca ggcaaacgcc caaccacact tggcctgggc
    15301 gatgggccaa acgatgcgcc cttactggag gtaatggatt acgcggtgat tatgaaaggg
    15361 ctaaaccgtg aaggggtgca tctgcatgat gaggatccgg cccgcgtctg gcgaacgcag
    15421 cgtgaaggac cggaaggctg gcgtgaaggg ctggaccatt ttttctccgc ccgttaagcg
    15481 ttatcgctcg cgaatacccg attgcgcccg gcctgtttag cgagataaag tcgacggtcg
    15541 gccagtgact ggagttgttc aaaatcataa tcaccggttt cctcgctgct gcttaccccc
    15601 agcgaggcac tgatgcgtat cgtcgtactc ttagcaatca acatctcttt ttcatttagc
    15661 ttcaggcgaa tacgttctgc aacctccgca gcctgcgtca gactcgcgcc tggcagaatc
    15721 acacaaaact cctcgccacc gacccgcccg gcaacgtcct gcgcacgcaa ggaactgcta
    15781 attaatccgg cagcatgaga aagaacacgg tcgcccgcct gatggccaaa gcggtcatta
    15841 atcgctttaa aatggtcaag gtcgacctga atgacagaaa aaggatgttg gtgcgtctga
    15901 cacaatttag cgagcgaacg ggctttttcg aacagtgcgc cactgttata taaacgcgtt
    15961 aaggtgtcgt gccacgcctg ccactgcaac aagctttgca aaacatacat gttgctgacc
    16021 atccggcgaa tcacatacca ggagatgagt aacatggtgg taaagagcgc ccacagcaga
    16081 gttaatgcaa tgctgatact gccgaaatca ccacgcacgc cttcgcttag cgtatggacg
    16141 cgcaccagca caccgtcgaa atggtccaga cgttcccagc taacatagcg actgttcata
    16201 cgaatgccgc cccgcgtgtc atgttccatt gcctgcgcca gcaaggccag ttcacgaggg
    16261 tcaaaaatat tccctgttgg atggtcagga ttggaagagg tcaataatct cagcttactg
    16321 tcatagagct gatactcacc atcgaggttt ttatcgatgg cgtttcttaa aaattgctgc
    16381 atggtacgca cgggaatact catccccagc acgccatacc agtagttttc actgtctacc
    16441 ggaacactga cggtaacctg cggttcagta ttgctggcgt gttccggttg tgaagtgaac
    16501 cagcgtaccg cgcggtgacg attttctcgt tgcgaatggc cgacaaacca gggttgggtg
    16561 acatagccgt aataacgcgt tggtacattg cgcgtaaata aggtcggctg cgtcgaaacg
    16621 taaaatccgg cacgcgagac atacatcgcc tgttcaacca tcgacgagga gttgtgcgcc
    16681 agtcgcagca agtaaccaac ttccagtgca gcggtaattt cattgtcgag gctttcattt
    16741 tcgcgagaca ggagattccc ctcgctgact aatgcatccg acacaccatt gactggcagg
    16801 gtgcgtcgtc ggttgagttc gatttgccag gcgtgctcgt cgcgatgctg ttcaaactcg
    16861 gtaacggcat tacgcagaga ggtgaaatcc agtggcgcta cgagagcttc gcgcatgcca
    16921 ttacgcaaaa agatcagttt gtcgacgtta tactgcaaat gcttatcgag cgcgttggct
    16981 acgttttcca gatgattacg ctggctggag atataggcat cttccagcac caccacttca
    17041 cgccaggtga gcaaggtgga aaaaagcaat accacgataa agcagagatt aacgacatga
    17101 ccaggcccca ggcggcgtgc gagttttttc aaccagctct ggttttccat ttttgtctcg
    17161 tgctgcaccc tgacgactcc caacctgctt ttttatgatt ctggcataac ccggcgataa
    17221 cgtaccgcaa aacagcgata attttataat ggtggcatga taagacgcga ctatcattca
    17281 ttgcaggcat tggcacacca ggccggatgt gaaccggcct ggtgttaatg ataccctttc
    17341 aaacttcttc cgcagaacga ctcccctccc ccggaacaag ctcggtttca tgaaaagctt
    17401 cccgcttgac cccataaccg tcataccaac gacactcaac cataccgctg gagtatccgg
    17461 tgacaatcat ccgcgggccg ccctctttaa ccgtaacttc ctcactaacc ataaagctca
    17521 tactcgcctc cttttttcga gtgaaacttg ttcaccttag ttgaagatgg cgaattttgc
    17581 ataatagcct gtgcgagtat taatgtgcct gaccgcgcat tttcgctact gctttcactc
    17641 ccagcaccac gatgccgccg atgataaacc caagaatcag atttaaaaca gtcggtaata
    17701 tcatcgccac cactgcactt tgctgcccgg cgaaatgttc aatggcgtga tgcagcggcg
    17761 caataccatg taccacaatc ccgccgccga cgaggaacat cgccagcgtg ccgacaatcg
    17821 ataacgcttt catcaaccag ggcgcgataa tcaataaccc tttacctaat gcctgcatca
    17881 gcgcgctgga tttttccgcc agccaatacc ccaggtcatc aatcttaacg ataaccccta
    17941 ccagaccata gacgcccacg gtcactacca gcgcgatgcc tgaaagcacc agcacctgat
    18001 taagcaacgg cgcttctgcc acaatcccca gcgtgatggc gacgatttcc gcagacaata
    18061 taaaatcggt acgaatcgcc ccttttattt tgtccttttc aaacttcagc ggatcctgcg
    18121 ccgccagctt ctccagacgc tgctgactct gcgccggatc ttctttatgt ttacgcgcct
    18181 ccagcatatg cagcactttc tctacccctt caaagcagag aaacgcgccg ccaatcatca
    18241 acagtggcgt aatcgcccac gggataaacg cactgatgat cagcgccagc ggcaccagaa
    18301 tcactttatt gatcagcgat cctttcgcca cgccccagac cacgggaagt tcccggttgg
    18361 cccgcacgcc tgaaacttgt tgcgcattga gcgataagtc atcccctaat acaccggcgg
    18421 ttttcttcgc cgccagtttg cccatcacgg aaatatcgtc cagcagtgtg gcgatatcat
    18481 cgagcaacgt cagtaaacta ctcccggcca aaagcattcc ctctcatttt tattgttgaa
    18541 gggtgtaagt atgaagcaaa aacggcagct ctgatacttg cttcacaggt caacattttt
    18601 ttaacaatca agtgcaatta tatctctcgc cagcagaatg attttcacgt ttactatgac
    18661 ccaccttttt atttccaccg tgagggatta tgcgtttccg ccagttgtta ccgctttttg
    18721 gcgcgctgtt tgcgttgtat atcatttggg gctcaaccta ttttgtcatt cggattggcg
    18781 tggaaagctg gccgccgtta atgatggcag gcgtgcgatt cctggcagcc ggtattttat
    18841 tgctggcatt tttgctactg cgcggacaca aactcccccc gctacgtccg ctgctcaatg
    18901 ccgcgctgat tggcctgtta ttgctggctg tcggtaatgg catggtaacg gttgccgaac
    18961 atcaaaatgt tccttccggc atcgccgccg tagtggttgc aaccgtgccc ctctttaccc
    19021 tgtgcttcag ccgcctgttt ggcattaaaa cgcgcaaact ggaatgggtg ggtattgcca
    19081 ttgggcttgc cggaatcatc atgctcaata gcggtggaaa tttaagcggc aatccgtggg
    19141 gcgcgattct gattttaatc ggctcgatta gctgggcgtt tggctcggtt tatggctcgc
    19201 gcattacctt acctgtaggg atgatggcgg gtgcgattga gatgctggcg gcaggcgtgg
    19261 tgttaatgat cgcgtcgatg attgcaggtg aaaaactgac ggcactccct tccctttcag
    19321 gcttccttgc ggtcggctat ctggcgctgt ttggttcgat tatcgccatt aacgcttata
    19381 tgtatttaat ccgcaacgtc agtccggctc tcgccaccag ctacgcctac gttaacccgg
    19441 tggtcgcggt cttgctgggt acgggactgg gcggagaaac gctgtcgaag attgaatggc
    19501 tggcgctcgg cgtaattgtc ttcgcggtgg tactggtcac gttgggaaaa tatctcttcc
    19561 cggcaaaacc cgtagttgcg ccagttattc aggacgcatc aagcgagtaa atgaatcccc
    19621 tgcgtgtcga tctgcgcact ggcaccttcg ccgcagatcc actcttccag acgctcgcta
    19681 agcgcttcat ccgtcagctt ctcgcgccca cgtaatgcac actcccagac aatcaatacg
    19741 cgacagccga gttcctgcaa acgactgata tcgcggcgat cgcgttcaac atttttacct
    19801 atcttctcca gccagaactc ggttcgagtc gcaggcactt taaacagata gcagtgatga
    19861 tgatgccaga agcagccgtg ggtaaatatc acgcagcgat attcatcaac gacaaaatcc
    19921 gggcgtccgg gcagactggc gtcctgaacg cgaaacgcca ggcccagccc ggttaacaga
    19981 ctggcgaggc gcttctctat cgccgtatca cgcgtggcaa tcgcgcgcat atttttgctg
    20041 cgagtggcct tatcgtgaac gtcggccatg ttgtgcctct tgctgacgca acgccaccgc
    20101 ctgtttgatt tttggctcaa gcagttttgc caccgcggca aagaccggca cgaccaccga
    20161 attaccgaac tggcgatagg cctgagtgtc cgaaaccgga atgcggaatt tcgcttctcc
    20221 cggcgcttca aaacccatta agcgcgcgca ttcccgaggc gttaaccgac gtggacgatg
    20281 ttgctgattc agcggatcgt caaaatcttt ctcacccgtg gccatatccc agccgcgatc
    20341 gatcaaaatt tccgcgccat ctttgtaata acgcgcagac aacgtacgcg tgacgctttg
    20401 cggattgttc ggataaacca ttccataacc gaagccatta ccgcgtgcct gatgtttttt
    20461 cgcataacga tagaggtact tccacagcac cggcgtcagg atatatttcg cctcgaccat
    20521 cgggtccagc agctgggcca gcgtcactcg ctgcgcaggg aaacattcgc tgatatcacg
    20581 cagagtaaaa tcggctttca gattcagatc gcgacgaaaa cccaccagca cgatgcgttc
    20641 acggtgctgc ggcaggaaat gtttgccatc gatgattttc ggatcgtccg gcccgttatc
    20701 ttcagcgtcg gcaacgtcat agcccagttc gtcaagcgtc tgcatgatga tgcggaacgt
    20761 tttaccctgg tcgtggcttt tcaggttttt gacattttcg agcacaaaaa tcgccggtcg
    20821 acgcgcgtcg ataatacgca ccacatcaaa gaacagcgtg ccctgggtat cacaggcaaa
    20881 accgtgcgcc cgcccgagcg agttcttttt cgatacgcca gccagcgaaa atggctgaca
    20941 aggaaaaccg gccagtaata catcgtgttc aggaatgtgt tgacgaatat gttccgctgc
    21001 cgcctcatca ctcacgccgt ctttatggct gagggtgatg tcgcggatat cttcattaaa
    21061 atgatgcgtc gccggatcgc aataatggtt ggctttataa gtgcgaaccg catgtttgtt
    21121 ccattcgctg gtaaagacgc actgtccgcc aatcgattca aaaccgcgac ggatgccgcc
    21181 aattccggca aatagatcga taaagcgaaa cgcataatgc ggatgatgtg ccggaggttt
    21241 gggtaacaac gtttgcagat gggcgaactc cttctcactt aaacggtgcc atgccgagtc
    21301 attcgccagc gcacgtttta aaattgccgc gctccagtga ttctcaccca caccattaag
    21361 ctgcgccacc agcgttttaa catcataaat ttgcagcagt ttctccagca ttgcctgtgc
    21421 ggcattcccg gtgctgtatg aatcggttac tgatatattt tcctgcatag atttaaccgg
    21481 ccatctgaga tggccgacag gttaacataa ttacagccat ttagggccac gcgctcagcc
    21541 cgcgggtgaa aacgcatcta tcaccttgtg atcgacacct tcattctccc ccgccagttc
    21601 ggcactgagc ttcgccataa actcgactaa aaattgcgcg ttgtgctgcg ccagctgctt
    21661 gcctcttgcg gtttgcatgg tttgcggtag cttcagcaac ttagtctgga aatgatccag
    21721 cgcgtagcgt ttatcatcaa gcgggcgatg ctgtgcgaac ggatcttcac catcaaacag
    21781 cgccacgccc aatgcccctg aaacggcaaa cacgcgcgcc aggccaatcg cccccagggc
    21841 ttccagccga tccgcatcct ggacgatttt agcttccgtt gttaaggggg cgatttgcgc
    21901 gctgaaactg tgagcggcaa tggcatgaca aacggcctcg attttctccg ccggaaattg
    21961 ctcgaactct tcacggagca ggcgacgcgt ctcttctgcc gccaggattg aagaacgctg
    22021 ccgttgcgga tgatttttcg ccaggctcac aatatcgtgg aaataacagg cggttaaaat
    22081 caccagcata tcgacgtcat catctgccgc cagcttctgt gccgtagccc agacgcggcg
    22141 aaaatgatac acgtcatggg ccgcgtcctg gtgctgatga tggtttttca accagttttc
    22201 gaactgtgtc tgccagtgtt gtaagtccat acgcgctccg ttgccaaaga gtcgccacct
    22261 tagcagtttt tattcatagc gtctgctacg tgcgggacga ggtcgggtgt accaggcaat
    22321 gctgcctaac aatgccccga ccagagccat aaataaaaat ccgaccagtg cccctaacca
    22381 ctttcccgcg gtagagccta aatcgctttc aaatcccgac acgggggcat ccggctcacc
    22441 cccagatgta acgcgccatt gcccagacga aatagccaca ccatgtatag aaagcccaca
    22501 aagccagttt gccaccaggg ctgcgagaga gcctctcttc catctttgtc gaaccgtatt
    22561 ccattttgag ataacccgga aaattcagac ttagtactat tatgtgataa aagtcacatt
    22621 tttccacatt gaatgagcca gtcaagactc aatcattttt tgaacaaata gataaatttt
    22681 gcgcaacggc agtgaataca ggaaaaatcg ttttgagcga ttttatttat ctttgatatc
    22741 ggctttatta attc
//
LOCUS       DAPHDW010000069        22540 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.069, whole genome shotgun sequence.
ACCESSION   DAPHDW010000069 DAPHDW010000000
VERSION     DAPHDW010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 22540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.069"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..734)
                     /locus_tag="RH057_002611"
     CDS             complement(<1..734)
                     /locus_tag="RH057_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDV3128962.1"
                     /translation="MSGKPAARQGDMTQYGGSIVQGSAGVRIGAPTGVACSVCPGGVT
                     SGHPVNPLLGAKVLPGETDIALPGPLPFILSRTYSSYRTKTPAPVGSLGPGWKMPADI
                     RLQLRDNTLILSDNGGRSLYFEHLFPGEDGYSRSESLWLVRGGVAKLDEGHRLAALWQ
                     ALPEELRLSPHRYLATNSPQGPWWLLGWCERVPEADEVLPAPLPPYRVLTGLVDRFGR
                     TQTFHREAAGEFSGEITGVTDGAGRH"
     gene            complement(894..1106)
                     /gene="rpmE"
                     /locus_tag="RH057_002612"
     CDS             complement(894..1106)
                     /gene="rpmE"
                     /locus_tag="RH057_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HDV3128963.1"
                     /translation="MKKDIHPKYEEITASCSCGNVMKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            1309..3507
                     /gene="priA"
                     /locus_tag="RH057_002613"
     CDS             1309..3507
                     /gene="priA"
                     /locus_tag="RH057_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HDV3128964.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     VVVSVSDVSELPLNGLKAVVEVLDVEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            3663..4688
                     /gene="cytR"
                     /locus_tag="RH057_002614"
     CDS             3663..4688
                     /gene="cytR"
                     /locus_tag="RH057_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HDV3128965.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            4780..5739
                     /gene="ftsN"
                     /locus_tag="RH057_002615"
     CDS             4780..5739
                     /gene="ftsN"
                     /locus_tag="RH057_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HDV3128966.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            5832..6362
                     /gene="hslV"
                     /locus_tag="RH057_002616"
     CDS             5832..6362
                     /gene="hslV"
                     /locus_tag="RH057_002616"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HDV3128967.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            6372..7703
                     /gene="hslU"
                     /locus_tag="RH057_002617"
     CDS             6372..7703
                     /gene="hslU"
                     /locus_tag="RH057_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HDV3128968.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQTIIIDADYVSKHLDALVADEDLSRFIL"
     gene            7770..8696
                     /gene="menA"
                     /locus_tag="RH057_002618"
     CDS             7770..8696
                     /gene="menA"
                     /locus_tag="RH057_002618"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HDV3128969.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQEMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            8789..9274
                     /gene="rraA"
                     /locus_tag="RH057_002619"
     CDS             8789..9274
                     /gene="rraA"
                     /locus_tag="RH057_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HDV3128970.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(9359..9604)
                     /gene="zapB"
                     /locus_tag="RH057_002620"
     CDS             complement(9359..9604)
                     /gene="zapB"
                     /locus_tag="RH057_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HDV3128971.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            10029..10874
                     /gene="glpF"
                     /locus_tag="RH057_002621"
     CDS             10029..10874
                     /gene="glpF"
                     /locus_tag="RH057_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
                     /protein_id="HDV3128972.1"
                     /translation="MSQTSTLKGQCIAEFLGTGLLIFFGVGCVAALKVSGASFGQWEI
                     SVIWGLGVAMAIYLTAGVSGAHLNPAVTIALWLFACFDKRKVIPFIVSQVAGAFCAAA
                     LVYGLYYNLFFDFEQTHHIVRGSVESVDLAGTFSTYPNPHINFVQAFAVEMVITAILM
                     GLILALTDDGNGVPRGPLAPLLIGLLIAVIGASMGPLTGFAMNPARDFGPKVFAWLAG
                     WGNVAFTGGRDIPYFLVPLFGPIVGAIVGAFAYRKLIGRHLPCDICVVEEKETTTPSE
                     QKASL"
     gene            10897..12405
                     /gene="glpK"
                     /locus_tag="RH057_002622"
     CDS             10897..12405
                     /gene="glpK"
                     /locus_tag="RH057_002622"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HDV3128973.1"
                     /translation="MTEKKYIVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPG
                     WVEHDPMEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAI
                     VWQCRRTAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSRERARRGE
                     LLFGTVDTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEV
                     RRSSEVYGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNT
                     GEKAVKSENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFA
                     TKVQNTNGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDV
                     LEAMQADSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGL
                     AVGFWQNLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            12541..13551
                     /gene="glpX"
                     /locus_tag="RH057_002623"
     CDS             12541..13551
                     /gene="glpX"
                     /locus_tag="RH057_002623"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HDV3128974.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            13648..14394
                     /gene="fpr"
                     /locus_tag="RH057_002624"
     CDS             13648..14394
                     /gene="fpr"
                     /locus_tag="RH057_002624"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HDV3128975.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(14399..14827)
                     /gene="uspD"
                     /locus_tag="RH057_002625"
     CDS             complement(14399..14827)
                     /gene="uspD"
                     /locus_tag="RH057_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HDV3128976.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(14854..15153)
                     /locus_tag="RH057_002626"
     CDS             complement(14854..15153)
                     /locus_tag="RH057_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HDV3128977.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(15365..15805)
                     /locus_tag="RH057_002627"
     CDS             complement(15365..15805)
                     /locus_tag="RH057_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV3128978.1"
                     /translation="MTIQQWLFSFKGRIGRRDFWIWIGLWLAGMLVLFSLAGKNLLDI
                     QTAAFCLVCLLWPTAAVTVKRLHDRGRSGAWAFLMIVAWMLLAGNWAILPGVWQWAVG
                     RFVPTLILVMMLIDLGAFIGTQGENKYGKDTQDVKYKADNKSSN"
     gene            15906..16505
                     /locus_tag="RH057_002628"
     CDS             15906..16505
                     /locus_tag="RH057_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HDV3128979.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            16613..17380
                     /gene="tpiA"
                     /locus_tag="RH057_002629"
     CDS             16613..17380
                     /gene="tpiA"
                     /locus_tag="RH057_002629"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HDV3128980.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(17435..18190)
                     /gene="cdh"
                     /locus_tag="RH057_002630"
     CDS             complement(17435..18190)
                     /gene="cdh"
                     /locus_tag="RH057_002630"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HDV3128981.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGRTQNHFHIHISCIRPDVREQLDNNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHQCEILR"
     gene            complement(18297..19286)
                     /gene="sbp"
                     /locus_tag="RH057_002631"
     CDS             complement(18297..19286)
                     /gene="sbp"
                     /locus_tag="RH057_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HDV3128982.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(19606..20568)
                     /gene="pfkA"
                     /locus_tag="RH057_002632"
     CDS             complement(19606..20568)
                     /gene="pfkA"
                     /locus_tag="RH057_002632"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HDV3128983.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(20749..21651)
                     /gene="fieF"
                     /locus_tag="RH057_002633"
     CDS             complement(20749..21651)
                     /gene="fieF"
                     /locus_tag="RH057_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312867.1"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HDV3128984.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLISPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(21859..22500)
                     /locus_tag="RH057_002634"
     CDS             complement(21859..22500)
                     /locus_tag="RH057_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128985.1"
                     /translation="MEKVIIELDNRWELADFAVLTKEYLQLYGFFYGLKGGSRGYSTM
                     PWEGGHSVVNFFRGAYSATPPDLRPVVKKIQYASPGFIELSALIDISWQIAELVTAVG
                     GSILAANKVYDQVMRTYRQREWAKLKSEKLRIQNQIKEIELVSDAVKSLESVMALSEE
                     QRKNLVQLSGADELVQLKILLAVYRRLSPLVELQNSGKANFSAGKNKNLKASD"
BASE COUNT         5585 a         5900 c         5768 g         5287 t
ORIGIN      
        1 aagtgacgcc cggcaccatc cgtcacgccg gtgatttcgc cgctgaattc accggcggct
       61 tcgcggtgga acgtctgtgt gcgcccgaag cggtccacca gcccggtcag tacccggtac
      121 ggcggcagcg gcgcaggcag cacctcatcc gcttccggca cccgctcaca ccagccgagc
      181 agccaccacg gcccctgcgg actgtttgtc gccagataac gatgcggact taagcggagt
      241 tcttccggca gcgcctgcca gagtgcggcc agccggtgac cttcatccag tttcgccacg
      301 ccgccgcgca ccagccacag tgactcgctg cggctgtaac cgtcctcacc gggaaacagg
      361 tgctcaaaat acaggcttct gccgccgtta tcactgagta tcagtgtgtt atcgcgcagc
      421 tgtaagcgga tatccgcagg cattttccag ccggggccga ggctccccac cggcgcgggc
      481 gtttttgtcc ggtaactgct gtaggtgcgg gagagaatga acggcagcgg gccgggcagg
      541 gcgatgtcgg tttcaccggg aaggaccttt gcaccgagca ggggattgac cggatggccg
      601 gacgtcactc cgccggggca caccgaacag gccacgccgg tgggggcacc aatgcgcacc
      661 ccggctgaac cctgaacaat gctaccgcca tactgcgtca tgtcgccctg acgcgctgcc
      721 ggttttccgc tcatcgtaaa atccttttga aataaataag aaccagaagc ctagcgccag
      781 tcaacaacag gaaatgataa tgagttaaca ccccggaatt tttcagaatt tataaaaggc
      841 aaattacagg cacaaaaaaa gcgccgtgcg gcgctttttt cggaaatccg gtcttatttg
      901 ctgcccggga tgttgaaacg cttgttgaag cggtcaacac ggccaccggt agcaacatca
      961 cgctgtttgc cagtgaagaa cgggtggcac ttgctgcaca cgtcgaggtt caggtcatga
     1021 ccaacggtgg agcggatttt cattacgtta ccgcaagagc agctagcagt aatttcttcg
     1081 tatttcgggt gaatatcttt tttcatggga aaacctcggt ttaaggccgc gtcgctcttc
     1141 cagccctaac gccagacacc acgcgatgtt aaaagtatag cttcaatacg atcatttcgt
     1201 acgaaggcgc gaaatcatac agaaattaac cagcgtatgc aaactgatcc gcactcttct
     1261 acggcaatgt gtatactaac ccaccgaatt tcaagtcagg atgatgctat gcccgttgcc
     1321 cacgttgcct tgcccgttcc gcttcctcgt acctttgact atctgctgcc agaaggcatg
     1381 acggttaaag ccgggtgtcg cgtgcgcgtg ccgtttggca aacagcagga gcgcatcggg
     1441 gttgtggtgt cagttagcga tgtcagtgag ctgccgctta acgggctgaa agcggtagtg
     1501 gaagtgctgg acgttgagcc ggtatttact cactccgtct ggcggttact gctctgggcg
     1561 gcagattact atcatcatcc gattggcgat gtgctgtttc atgccttgcc gattttactg
     1621 cgccaggggc ggcctgcggc gaacgcgccg atgtggtact ggtttgccac tgaacaaggc
     1681 caggcggtgg atctgaacag cctgaaacgc tcccccaagc aacaacaggc gctggcggcg
     1741 ttacggcaag gcaaaatctg gcgcgaccag gtcgccacgc tcgaatttaa tgatgccgcg
     1801 ttgcaggcgc tacgcaaaaa aggtctgtgt gatttagcaa gtgaaacacc agagtttagc
     1861 gactggcgaa cgaactatgc cgtttctggt gagcggttgc gattgaatac cgaacaggcc
     1921 accgccgttg gcgcaattca tagcgcggca gatacttttt ctgcctggct gctggcgggc
     1981 gttaccggtt ccggtaaaac ggaggtttat ctcagtgtac tggaaaacgt gctcgctcag
     2041 ggcaaacagg cgctggtgat ggtgccggaa atcggcctga caccgcaaac tatcgcccgt
     2101 tttcgtgaac gttttaatgc ccccgtggaa gttctgcatt ccggcctgaa cgacagcgag
     2161 cggctttcgg cgtggttgaa agcgaaaaat ggcgaggcgg cgattgtgat cggcacccgc
     2221 tccgcgctgt ttacgccgtt taaaaatctc ggcgtgattg tcattgatga agagcacgac
     2281 agctcctaca agcagcagga aggctggcgc tatcatgccc gcgacctggc ggtgtatcgt
     2341 gcgcatagcg agcaaatccc gattattctt ggctccgcaa cgcccgcgct ggaaacgttg
     2401 tgcaacgtcc agcagaaaaa ataccgcctg ctgcgcctga cgcgccgggc ggggaatgcg
     2461 cgtccggcaa ttcaacacgt gctggattta aaaggtcaga aggtgcaggc aggtctggct
     2521 ccggcgttaa tcacccgtat gcgccagcat ttacaggcca acaatcaggt tatcctcttt
     2581 cttaatcgtc ggggttttgc gcctgcgcta ctgtgccacg actgcggctg gattgccgaa
     2641 tgcccacgtt gcgatcacta ctacacgctg catcaggcgc aacagcattt gcgctgtcac
     2701 cactgtgaca gtcagcgtcc ggtgccgcgc cagtgccctt cctgcggttc cacgcacctg
     2761 gtacccgtgg ggctgggcac cgaacagctt gaacagacgc ttgcgccgct gttccctgat
     2821 gtgcccattt ctcgtatcga ccgcgatacc accagccgca aaggggcgct ggaacagcaa
     2881 ctggcggaag tacatcgcgg cggtgcgagg attttgatcg gcacacaaat gctggcgaaa
     2941 ggtcaccact ttccggatgt gacgctggtg gcgttactgg atgtggacgg cgcgctgttt
     3001 tctgccgatt ttcgctcggc agaacgtttc gcccagcttt acacccaggt cgccggtcgt
     3061 gccgggcgtg cgggtaaaca gggcgaagtg gtgctgcaaa cgcaccatcc ggaacatcct
     3121 ctgttgcaaa cgttgctcta taaaggctac gacgcctttg ccgaacaggc gctggctgag
     3181 cgacgcatga tgcagctgcc gccgtggacc agccatgtga ttgtgcgtgc ggaagatcat
     3241 aacaatcagc atgcgccgct gttcctgcaa caactgcgta atctgatcct ttccagcccg
     3301 ctggcagacg acaaactgtg ggttctcggc ccggttccgg ctctggcacc taagcgtggt
     3361 ggccgctggc gctggcagat cttgttgcag cacccttccc gcgtgcgctt gcaacacatc
     3421 attagtggta cgctggcgct tattaataca ataccggatt cccgcaaggt gaaatgggtg
     3481 ttggatgtcg atcccatcga aggctaatgc gcaaacgttg cgaggcggat cgaaaaattc
     3541 aatattcatc acacttttca tgaaaattct gtaaccgttt tcacgcgcta tctgctaaaa
     3601 atgttgccga tgtgaagtaa acatggatgt agtacgcctg acgtgccagg cgaggagtga
     3661 gtgtgaaagc gaagaagcag gaaactgccg cgaccatgaa agacgttgcc ctcaaggcaa
     3721 aagtctctac agcgactgtc tcccgagcat taatgaatcc cgataaagtc tcccaggcca
     3781 cccgtaatcg ggttgaaaaa gcggcccggg aagtgggtta tttaccgcag cctatggggc
     3841 gcaacgtcaa gcgtaatgaa tcccgcacca ttctggtgat tgtcccggat atctgcgatc
     3901 ccttctttag cgaaattatt cgcggtatcg aagttacggc ggcaaatcac ggatatctgg
     3961 tgctgattgg cgactgtgcg catcaaaatc agcaggaaaa aacctttatc gatttgatca
     4021 tcaccaagca aattgatggc atgttgctgc tgggttcaag gctgccgttt gatgccagca
     4081 ttgaggaaca gcgtaatctg ccgccgatgg tgatggcgaa cgaattcgca ccggaactgg
     4141 agctacctac agttcatatc gacaatctga ccgccgcatt tgatgcagta aattatctat
     4201 atgagcaagg gcataaacgg attggctgta tagccggtcc cgaagagatg ccgctgtgtc
     4261 actaccgcct gcaaggttac gttcaggcgc tgcgtcgctg cggcattatg gttgatccgc
     4321 aatacatcgc ccgtggcgac ttcaccttcg aagccggaag caaagcgatg cagcagctgc
     4381 ttgatcttcc acaaccgcct actgctgtct tctgccatag cgatgtgatg gcgctcggcg
     4441 cactttctca ggcaaaacgc caggggctga aagtcccgga agacctttcc ataatcggtt
     4501 ttgataacat cgacctgacg caattttgtg atccgccgct gacaaccatc gcgcagccgc
     4561 gttacgaaat cggtcgggaa gctatgctgt tattgcttga tcaaatgcag gggcaacacg
     4621 ttggcagtgg ctctcgttta atggactgcg aacttatcat ccggggatca acacgcgcgt
     4681 taccttaaag taaaccttta agactgccgt gactggtcaa aggcccgccg cttaagtaac
     4741 atggcgggct gacgaacgaa taaatacagc gaaacgatag tggcacaacg agattatgta
     4801 cgccgcagcc aaccggcacc ttcgcggcga aaaaagagca cctcacggaa aaagcaacga
     4861 aatctgcctg cggtttctcc cgctatggtc gctattgctg ccgccgttct tgtgaccttt
     4921 atcggtggtc tgtacttcat tacgcatcac aagaaagaag agtccgagac gctgcaaagc
     4981 cagaaagtga ccggtaacgg actaccacca aaaccagaag aacgctggcg ctacattaaa
     5041 gagctggaaa gtcgccagcc gggtgtgcgt gcgcccacag aaccttctgc cggtggtgaa
     5101 gtgaaaacgc cggagcaact gacaccagaa caacgtcagc ttcttgaaca aatgcaggct
     5161 gatatgcgcc agcagccaac gcagctggtt gaagttccgt ggaatgaaca gacgccggaa
     5221 cagcgtcagc aaacgctaca acgccagcgt caggcgcagc aattagcgga gcagcaacgc
     5281 ctggctcagc agtcgcgtac gactgaacaa agctggcagc agcagacgcg tacgtcgcaa
     5341 gccgcgccgg tacaggcgca accgcgccag tctaaaccgg cttctactca gcagccgtac
     5401 caggatctgc tgcaaacccc tgcgcacacg accgcgcagt cgaagccgca gcaagctgcg
     5461 ccggttgctc gtgccgctga cgcgccaaaa ccgacggcgg agaaaaaaga cgaacgccgc
     5521 tggatggtgc agtgcggttc gttcagaggc gcggaacagg cagagacggt acgtgctcag
     5581 ctggcgttcg aaggatttga ctcgaaaatc accaccaaca atggctggaa tcgtgtggtc
     5641 attggcccgg tgaaaggcaa agagaacgca gacagcaccc tcaatcggtt gaagatggcg
     5701 ggtcatacaa actgcattcg gctcgccgcc gggggttgaa accctcaaaa tcccccccat
     5761 ctataattgc attatgcccc gtacttttgt acggggtttg tactctgtat tcgtaaccaa
     5821 ggggtcagct cgtgacaact atagtaagcg tacgccgtaa cggccatgtg gtcatcgctg
     5881 gtgatggtca ggccacgttg ggcaataccg taatgaaagg caacgtgaaa aaggtccgcc
     5941 gtctgtacaa cgacaaagtc atcgcgggct ttgcgggcgg tactgcggat gcttttacgc
     6001 tgttcgaact gtttgaacgt aaactggaaa tgcatcaggg ccatctggtc aaagccgccg
     6061 ttgagctggc gaaagactgg cgtaccgatc gcatgctgcg caaacttgaa gcactgctgg
     6121 cagtcgcgga tgaaactgca tcgcttatca tcaccggtaa cggtgacgtg gtgcagccag
     6181 aaaacgatct tattgctatc ggctccggcg gcccttacgc ccaggctgcg gcgcgtgcac
     6241 tgttagaaaa tactgaactg agtgctcgtg aaattgctga aaaggcgttg gatattgcag
     6301 gcgacatttg catctatacc aaccatttcc acaccatcga agaattaagc tacaaagcgt
     6361 aaggatctcc catgtctgaa atgaccccac gcgaaatcgt cagcgaactg gataagcaca
     6421 tcatcggcca ggacaacgcc aagcgttctg tggcgattgc tctgcgtaac cgctggcgtc
     6481 gcatgcagct caacgaagag ctgcgccatg aagtgacccc gaaaaatatc ctgatgatcg
     6541 gcccgaccgg tgtcggtaaa actgaaatcg cccgtcgtct ggctaagctg gcgaatgcgc
     6601 cgttcatcaa agttgaagcg accaaattca ccgaagtggg ctacgtcggt aaggaagtgg
     6661 attctattat tcgcgatctg accgatgccg ccgtgaaaat ggtacgcgtc caggctatcg
     6721 agaaaaaccg ttatcgcgct gaagaactgg cagaagaacg tattctcgac gtgctgatcc
     6781 cacctgctaa aaacaactgg ggacagaccg aacagcagca ggaaccgtcc gctgctcgtc
     6841 aggcattccg caaaaaactg cgtgaaggcc agcttgatga caaagaaatc gagatcgatc
     6901 ttgccgcagc accgatgggc gttgaaatta tggctcctcc aggcatggaa gagatgacca
     6961 gccagctgca gtccatgttc cagaacctgg gcggccagaa gcaaaaagcg cgtaagctga
     7021 aaatcaaaga cgccatgaag ctgctgattg aagaagaagc ggcgaaactg gtgaacccgg
     7081 aagagctgaa gcaagacgct atcgacgctg ttgagcagca cgggatcgtg tttatcgacg
     7141 aaatcgacaa aatctgtaag cgcggcgagt cttccggtcc ggatgtttct cgtgaaggcg
     7201 ttcagcgtga cctgctgccg ctggtagaag gttgcaccgt ttccaccaaa cacgggatgg
     7261 tcaaaactga ccacattctg tttatcgctt ctggcgcgtt ccagattgcg aaaccgtctg
     7321 acctgatccc ggaactgcaa ggtcgtctgc caatccgcgt tgaactgcag gcgctgacca
     7381 ccagcgactt cgagcgtatt ctgaccgagc cgaatgcctc tatcaccgtg cagtacaaag
     7441 cactgatggc gactgaaggc gtaaatatcg agtttaccga ctccggtatt aagcgcatcg
     7501 cggaagcggc atggcaggtg aacgaatcta ccgaaaacat cggtgctcgt cgtttacaca
     7561 ctgttctgga gcgtttaatg gaagagattt cctacgacgc cagcgattta agcggtcaaa
     7621 ctatcatcat tgacgcagat tatgtgagca aacatctgga tgcgttggtg gcagatgaag
     7681 atctgagccg ttttatccta taatcgcgtt caatcatttt catcattgtt tgatggggct
     7741 gaaaggcccc atttttattg gcgcgtatta tgactgaaca acaaattagc cgaactcagg
     7801 cgtggctgga aagtttacga cctaaaaccc tccccctcgc ctttgctgca attatcgtcg
     7861 ggacggcgct ggcatggtgg caaggtcact tcgatccgct ggtcgccctg ctggcactaa
     7921 ttaccgccgg gctattacag atcctttcta acctcgccaa tgattacggc gatgcggtaa
     7981 aaggcagcga taaacctgac cgcattgggc cgctacgcgg catgcaaaaa ggggtcatta
     8041 cccagcaaga gatgaaacgg gcgctcatta ttaccgtcgt gctcatctgt ctctccgggc
     8101 tggcactggt tgcagtggca tgccatacgc tggccgattt tgtcggtttc ctgattcttg
     8161 gcgggttgtc gatcattgcc gctatcacct acaccgtggg caatcgtcct tatggttata
     8221 tcggtctggg tgatatttcc gtactggttt tctttggctg gttgagtgtc atggggagct
     8281 ggtatttaca ggctcataca ttgattccgg cactgatcct tccggcgacc gcctgtggcc
     8341 tgctggcaac ggcagtactg aatattaata acctgcgtga tatcaatagc gaccgcgaaa
     8401 atggcaaaaa cacgctggtg gtgcgcttag gtgaagtgaa cgcgcgtcgt tatcatgcct
     8461 gcctgctgat gggctcgctg gtgtgtctgg cgctgtttaa tctcttttcg ctgcatagcc
     8521 tgtggggctg gctgttcctg ctggcggcac cattactggt gaagcaagcc cgttatgtga
     8581 tgcgggaaat ggacccggtg gcgatgcgac caatgctgga acgtactgtc aagggagcgt
     8641 tactgactaa cctgctgttt gttttaggga tattcctaag ccagtgggca gcataactga
     8701 caaatatcaa ttaacaattg atgattttgc caacagccta catagcgcga tatactgaaa
     8761 attctcgcag caactgaatg ttaagcctat gaaatacgat acttcggagc tttgtgacat
     8821 ctatcaagaa gatgttaacg tcgtggaacc gctgttctcc aactttggcg gacgggcgtc
     8881 gtttggcgga caaataatca cggtaaaatg tttcgaggac aacgggttgc tgtacgatct
     8941 gctcgaacag aatggccgtg gtcgtgttct tgtcgtcgat ggcggtggtt ctgttcgtcg
     9001 cgcactggtc gatgctgaac tggcgcgtct ggcagtacaa aatgaatggg aaggtctggt
     9061 catttacggc gcggtgcgtc aggtagatga cctggaagag ttggatatcg gcatccaggc
     9121 gatggcggca attccggttg gtgccgctgg cgaaggcatt ggcgaaagcg atgtccgcgt
     9181 caattttggc ggtgtcacct tcttctccgg cgaccatctt tatgccgaca ataccgggat
     9241 tattctttct gaagatccgc tggatattga atgatagaaa aggcaccgca aggtgccttt
     9301 tttctgcgtt acctgttggc ctacacagta aagaaattac gcggaagatg aagcgtaatc
     9361 agacctcttc catgcgaccc agcagggcct gcagacgttc ctgccagccg ttctgctgtt
     9421 ctttcagatg gttgttctca cgctccagct cttcgcgctg atgctgggca ttttgaactt
     9481 cctgcgacag tgagttgttt ttttctttca gctcttcgat ttccatctgc aacagagtga
     9541 tggtatcaat cgcctgctgt acttttgctt ccagtttctc aaacacttct aatgacattg
     9601 tcatacctct cctgaattgc aaggcgttga tggataaaaa tcctcgtccc gattaccggt
     9661 gacgccttaa taaatacgag cgcactttag ttagctccga ttgtatgaag ccgcgccatc
     9721 gctgtccagc ggcacgcctt gcagattacg gtttgccaca cttttcatcc ttctcctggt
     9781 gacataatcc acatcaatcg aaaatgttaa taaatttgtt gcgcgaatga tctaacaaac
     9841 atgcatcatg tacaatcaga tggaataaat ggcgcgataa cgctcatttt atgacgaagc
     9901 acacacattt taagttcgat atttctcgtt tttgctcgtt aacgataagt ttacagcatg
     9961 cctacaagca tcgtggaggt ccgtgacttt cacgcataca acaaacatta actcttcagg
    10021 atccgattat gagtcaaaca tcaaccttga aaggccagtg cattgctgaa ttcctcggta
    10081 ccgggttgtt gattttcttc ggtgtgggtt gcgttgcagc actaaaagtc tctggtgcgt
    10141 cttttggtca gtgggaaatc agtgtcattt ggggactggg ggtggcaatg gccatctacc
    10201 tgaccgcagg ggtttccggc gcgcatctta atcccgctgt taccattgca ttgtggctgt
    10261 ttgcctgttt cgacaagcgc aaagttattc cttttatcgt ttcacaagtt gccggcgctt
    10321 tctgtgctgc ggctttagtt tacgggcttt actacaattt atttttcgac ttcgagcaga
    10381 ctcatcacat tgttcgcggc agcgttgaaa gtgttgatct ggctggcact ttctctactt
    10441 accctaatcc tcatatcaat tttgtgcagg ctttcgcagt tgagatggtg attaccgcta
    10501 ttctgatggg gctgatcctg gcgttaacgg atgatggcaa cggtgtacca cgcggccctt
    10561 tggctccctt gctgattggt ctactgattg cggtcattgg cgcatctatg ggcccattga
    10621 caggttttgc catgaaccca gcgcgtgact tcggtccgaa agtctttgcc tggctggcgg
    10681 gctggggcaa tgtcgccttt accggcggca gagacattcc ttacttcctg gtgccgcttt
    10741 tcggccctat cgttggcgcg attgtaggtg catttgccta ccgcaaactg attggtcgcc
    10801 atttgccttg cgatatctgt gttgtggaag aaaaggaaac cacaactcct tcagaacaaa
    10861 aagcttcgct gtaatatgac tacgggacaa ttaaacatga ctgaaaaaaa atatatcgtt
    10921 gcgctcgacc agggcaccac cagctcccgc gcggtcgtaa tggatcacga tgccaatatc
    10981 attagcgtgt cgcagcgcga atttgagcaa atctacccaa aaccaggttg ggtagaacac
    11041 gacccaatgg aaatctgggc cacccaaagc tccacgctgg tagaagtgct ggcgaaagcc
    11101 gatatcagtt ccgatcaaat tgcagctatc ggtattacga accagcgtga aaccactatt
    11161 gtctgggaaa aagaaaccgg caagcctatc tataacgcca ttgtctggca gtgccgtcgt
    11221 accgcagaaa tctgcgagca tttaaaacgt gacggtttag aagattatat ccgcagcaat
    11281 accggtctgg tgattgaccc gtacttttct ggcaccaaag tgaagtggat cctcgaccat
    11341 gtggaaggct ctcgcgagcg tgcacgtcgt ggtgaattgc tgtttggtac ggttgatacg
    11401 tggcttatct ggaaaatgac tcagggccgt gtccatgtga ccgattacac caacgcctct
    11461 cgtaccatgt tgttcaacat ccataccctg gactgggacg acaaaatgct ggaagtgctg
    11521 gatattccgc gcgagatgct gccagaagtg cgtcgttctt ccgaagtata cggtcagact
    11581 aacattggcg gcaaaggcgg cacgcgtatt ccaatctccg ggatcgccgg tgaccagcag
    11641 gccgcgctgt ttggtcagtt gtgcgtgaaa gaagggatgg cgaagaacac ctatggcact
    11701 ggctgcttta tgctgatgaa cactggcgag aaagcggtga aatcagaaaa cggcctgctg
    11761 accaccatcg cctgcggccc gactggcgaa gtgaactatg cgttggaagg tgcggtgttt
    11821 atggcaggcg catccattca gtggctgcgc gatgagatga agttgattaa cgacgcctac
    11881 gattccgaat atttcgccac caaagtgcaa aacaccaatg gtgtgtatgt ggttccggca
    11941 tttaccgggc tgggtgcgcc gtactgggac ccgtatgcgc gcggggcgat tttcggtctg
    12001 actcgtgggg tgaacgctaa ccacattata cgcgcgacgc tggagtctat tgcttatcag
    12061 acgcgtgacg tgctggaagc gatgcaggcc gactctggta tccgtctgca cgccctgcgc
    12121 gtggatggtg gcgcagtagc aaacaatttc ctgatgcagt tccagtccga tattctcggc
    12181 acccgcgttg agcgcccgga agttcgcgaa gtgactgcat tgggtgcggc ctatcttgct
    12241 ggtctggcgg tcggtttctg gcagaacctc gacgagctgc aagagaaagc ggtgattgaa
    12301 cgcgagttcc gtccaggcat cgaaaccact gagcgtaatt accgttacgc aggctggaaa
    12361 aaagcggtca aacgcgcgat ggcgtgggaa gaacacgacg agtaatgtaa atgccggatg
    12421 aagcgtttat gccgcatccg gtagtcctga aaccgtgcgg gggcaacccc gcacacatca
    12481 ataatcccac ccttcccctg tgctacactt cgcgccattc cttactgctt agagtttgct
    12541 atgagacgag aacttgccat cgaattttcc cgcgtcaccg aatctgcggc gctggctggc
    12601 tacaaatggt taggacgcgg cgataaaaac accgcggacg gcgcggcggt aaacgccatg
    12661 cgtattatgc tcaaccaggt caacattgac ggcaccatcg tcattggtga aggtgaaatc
    12721 gacgaagcac cgatgctcta cattggtgaa aaagtcggta ctggtcgcgg cgacgcggta
    12781 gatattgctg ttgatccgat tgaaggcacg cgcatgacgg cgatgggcca ggctaacgcg
    12841 ctggcggtgc tggcagtagg cgataaaggc tgcttcctca atgcgccgga tatgtatatg
    12901 gagaagctga ttgtcgggcc gggagccaaa ggcaccattg atctgaacct gccgctggcg
    12961 gataacctgc gcaatgtagc ggcggcgctc ggcaaaccgt tgagcgaact gacggtaacg
    13021 attctagcta aaccacgcca cgatgccgtt atcgctgaaa tgcagcaact cggcgtacgc
    13081 gtatttgcta ttccggatgg cgacgttgcg gcctcaattc tcacctgtat gccagacagc
    13141 gaagttgacg tgctgtacgg tattggtggc gcgccggaag gcgtagtttc tgcggcggtg
    13201 atccgcgcat tagatggcga catgaacggt cgtctgctgg cgcgtcatga cgtcaaaggc
    13261 gacaacgaag agaatcgtcg cattggcgag caggagctgg cacgctgcaa agcgatgggc
    13321 atcgaagccg gtaaagtatt gcgcctgggc gatatggcgc gcagcgataa cgtcatcttc
    13381 tctgccaccg gtattaccaa aggcgatctg ctggaaggca ttagccgcaa aggcaatatc
    13441 gcgactaccg aaacgctgct gatccgcggc aagtcacgca ccattcgccg cattcagtcc
    13501 atccactatc tggatcgcaa agacccggaa atgcaggtgc acatcctctg attgatttga
    13561 tcgattgagc cttccagtcc ttcgggactg gaattttttt gttcggagaa cgaagataag
    13621 gcaagttaat caaaacagga gaaaaacatg gctgattggg taacaggcaa agtcactaaa
    13681 gtgcagaact ggaccgacgc cctgtttagt ctcaccgttc acgcccccgt gcttccgttt
    13741 accgccgggc aattcaccaa gcttggcctt gaaatcgacg gcgaacgcgt ccagcgcgcc
    13801 tactcctatg ttaactcgcc cgataatccc gatctggagt tttacctggt caccgtcccc
    13861 gatggcaaat taagcccacg attggcggca ctgaaaccag gcgatgaagt gcaggtggtt
    13921 agcgaagcag ctggcttctt tgttctggat gaagtaccgg actgcgaaac gctatggatg
    13981 ctggcaaccg gtacagcgat tggcccttat ttatcgattc tgcaactagg caaagattta
    14041 gatcgcttca aaaatctggt cctggttcac gccgcacgtt atgccgccga cttaagctat
    14101 ttgccactga tgcaagaact ggaaaaacgc tacgaaggga aactgcgcat tcagacggtg
    14161 gtcagtcggg aaacggcagc ggggtcgctc accgggcgga taccggcgtt aattgaaagt
    14221 ggggaactgg aaagcgcgat tggcctgccg atgaataaag aaaccagcca tgtgatgctg
    14281 tgcggcaatc cacagatggt gcgcgataca caacagttgc tgaaagagac ccggcagatg
    14341 acgaaacatt tacgtcgccg accgggccat atgacagcgg agcattactg gtaagcggtt
    14401 acttatcgat aaacggcacg atgagcaaat ccgcactcat cttattgatc atcccgcgat
    14461 atgccggcat caaacggttg ataaatgagt gatgatgacc acagacaagg aggtcgcact
    14521 gctctttttg cataatttcc agcagtgttt ccggcatttc tccgcgttca atacgcagtt
    14581 ttgtcttcgg ccattgaata tttttcgtca gtttatacag cttgttatcc gacttattct
    14641 tcaacaattg aagaatatct tctgttgcag ggaagtagat acccggatac aactcgctta
    14701 agccatcatc aatatgaatt aacgtcaggt gagcgtcatt atgtctggcg agctccagag
    14761 ctttattcac cagtaaggca tcttcttcat tcccggaaat tgccacgcca atgtgtttat
    14821 aagccatgtt taactccttc taaagcaact ccatcaagct aacgaacgca gtgataactc
    14881 aaaaataatc atctcggcct ggcatgagaa agtgaaggcc gcatcaagct caacttgccc
    14941 ctccagatcc ttaatcttat aggccacgct ggccccttca tctgcagagg atgcagcggc
    15001 ggcaagctct ttcagtttgg tcataaagga ctctgcttct tcgcgggtgg caaaaaagcg
    15061 cgaaaactta ctggtacagt tgtcgttatc aatcaccgta ccgatatcaa tcgcacatcc
    15121 tttagtactg catttatcta cgacatcttt catagggcac ctctgtatat tcgcctctct
    15181 gtcgattacg acttaagaat aatgcgccgt taagggaaga acaaacaagt aatgatctta
    15241 tggattacca ttttttttga tattgcttaa ggtgaagagt tcagaaggat tttccgggca
    15301 ttgtcatttg catcgtggaa ataacaatcc cggaaaaaaa tagcagtaag agatatatta
    15361 aaggttaatt acttgattta ttgtcggctt tatacttcac atcctgggta tctttaccat
    15421 atttattttc gccttgtgtg ccaatgaacg ctccaagatc gataagcatc atcaccagaa
    15481 tcaacgtcgg gacaaaacgc cccaccgccc attgccagac acccggtaaa atcgcccagt
    15541 tacccgccag cagcatccac gccacaatca tcagaaatgc ccatgcgccg gaacgcccgc
    15601 gatcatgcaa gcgcttaacg gttactgccg ctgttggcca aagcaagcac accaggcaaa
    15661 acgccgcggt ctgaatatcg agtaaatttt tacccgccag tgagaaaagc accagcatgc
    15721 ctgctaacca caggcctatc caaatccaga aatcacggcg tccaatacgc cctttaaatg
    15781 agaataacca ttgctgtatg gtcatgtaag ttccttgatg gttgtctttt ccaggattct
    15841 acccttttga caagggcgac agatatcgtt ttaatcggag ccagtcataa caaaaggtac
    15901 tgtcaatgaa gccagggtgt acgctgtttt ttctcttatg ttctgcatta accgttacaa
    15961 caacggcgca tgcacaaaca ccagatacgg caacgaccgc gccttatctg ctggctggag
    16021 cccctacttt cgatctctcc atcagccagt ttcgagaaga ctttaacagc cagaatccta
    16081 gcctgccact gaacgaattt cgtgccatcg acagcagtcc cgacaaagcc aatctcactc
    16141 gtgctgcaag taaaattaac gagaacttgt atgcttctac agcgctggag cgcggtacct
    16201 taaaaatcaa aagcattcaa atgacctggc tacccatcca ggggccagag caaaaagcag
    16261 cgaaagcgaa agctcaggaa tacatggcag cagtgatccg cacactcacc ccattaatga
    16321 ccaaaacaca aagccagaaa aaactgcagt cgctactaac ggcggggaaa aacaaacgtt
    16381 attacaccga gacagaaggt gcactgcgtt atgttgtcgc ggacaacggc gaaaaggggc
    16441 tgaccttcgc tgttgaaccg attaagctgg cgctatctga atcgcttgaa ggtttgaata
    16501 aatgacaaaa agcaaagcct ttgtgccgat gaatctctat actgtttcac agacctgctg
    16561 ccctgcgggg cggccatctt cctttattcg cttataagcg tggagaatta aaatgcgaca
    16621 tcctttagtg atgggtaact ggaaactgaa cggcagccgc cacatggttc acgagctggt
    16681 ttctaacctg cgtaaagagc tggcaggtgt tgctggctgt gcggttgcaa tcgcaccacc
    16741 ggaaatgtac atcgatatgg cgaagcgcga agctgaaggc agccacatca tgctgggtgc
    16801 gcaaaacgtg gacctgaacc tgtccggcgc attcaccggt gaaacttctg ccgctatgct
    16861 gaaagacatc ggcgctcagt acatcatcat cggccactct gaacgtcgta cttaccacaa
    16921 agagtctgac gaactgatcg cgaaaaaatt cgcggtgctg aaagagcagg gcctgactcc
    16981 ggttctgtgc atcggtgaaa ccgaagctga aaacgaagcg ggcaaaactg aagaagtttg
    17041 cgcacgtcag atcgacgcgg tactgaaaac tcagggtgct gcggcattcg aaggtgcggt
    17101 tatcgcttac gaacctgtat gggcaatcgg tactggcaaa tctgcaactc cggctcaggc
    17161 acaggctgtt cacaaattca tccgtgacca catcgctaaa gttgacgcta acatcgctga
    17221 acaagtgatc attcagtacg gcggctctgt aaacgcgtct aacgctgcag aactgtttgc
    17281 tcagccagat atcgacggcg cgctggttgg cggtgcttct ctgaaagctg acgctttcgc
    17341 agtgatcgtt aaagctgcag aagcggctaa acaggcttaa gtctgacagg tgccggattt
    17401 catatccggc acttactttc cttaactctt cgccttaacg caaaatctca cactgatgat
    17461 cctgaatttc ctcggctgaa gcacggttaa gcgtcagtag atttcgttgt gtcgccagca
    17521 atacaaatga gttatcactc tgccgtacca tcgccagccc gtagcttccc atatgttccc
    17581 gcgcctcagg tacttcttct gccagcatca taaatgggct gcgttgtacc agttcgcttt
    17641 ccgttacccg acgcgccagg tattcatgcc cgcgcaaacc acctggcagt ggcaaccagc
    17701 ggctgctgat gttcgccaga ttgttatcca gctgttcgcg cacatcagga cgaatacaag
    17761 agatatgaat atgaaaatgg ttttgcgtac gcccggtgcg ggagttgatc gccaaagaaa
    17821 ccgcgcgatc gggaaccggc tggccgtatt ttttgctcat aaaatcacgc gcctgccagg
    17881 ccaaccaaaa gaagttcggc gttgaaggat cggtcaacaa aggactttca gtaccgttaa
    17941 tacgatacgt tggcatcaac agatattgca gtggaccatt aagatctttt aaaaccacgt
    18001 atccggcatt gggtttgact tccgcacatg gcgaaggatt ttgattttgc tgctgattgg
    18061 gcaaacattc ctcaaggaca atcttacgta atgtatccga ctcttcaccg gttaatttcc
    18121 agtaaccaat accggcagcc acaacggcga taactatcat caccaaaaaa agaagacccg
    18181 cttttttcat ctttttttcc ctgtacctca aagagatgca agggtaacgc aaaatcgtga
    18241 caaataaaaa acccggtcgc gagaaaccac cgggtttgat aattatcctg gggaaatcag
    18301 cgtttgctga tctgatcgaa cgtaccgccg ttagcaaaat gctctttttg cgctttcgtc
    18361 cagccgccga actcttcatc aatggtgaat aacttcagct ttggaaacgc attttcgtac
    18421 tttttcgcta cttcagcgtc gcgcggacgg tagtagtttt tcgcggcaat ttcctgacct
    18481 tctggcgagt agagatattt caggtaggct tccgccacct ctttggtgcc ttttttctcg
    18541 accactttat cgaccaccga cacggttggc tctgcgagga tagactcact cggcgtgacg
    18601 atttcgaatt tatctttccc cagttcattc gctgccagca gagcttcgtt ttcccaagcg
    18661 atcagtacat cgccaatccc gcgctcgaca aaggtgttag tggagccgcg cgcgccagaa
    18721 tccagaactt cgacgttttt atacagtgcc cgaacaaaat cctgtgcttt tgcctgatcg
    18781 ttgttgttgt gatgcagcgc gtagccccag gcagccaggt agttccagcg tgcgccacca
    18841 gagcttttcg gattaggcgt gatcaccgaa acacccggtt taatcagatc gttccagtca
    18901 tggatctgct tcggattgcc cttacgcacc aggaaaacaa tggtggaagt gtacggtgcg
    18961 gagttatccg gcagacgttt gatccactct ttatcaatcc gcccgcgttc cgcaatcgcg
    19021 tccacgtcat aggccagagc cagcgtgaca acatcagctt caataccgtt gattaccgac
    19081 gtcgcttgtt tacctgaacc accgtgtgac tgacgaatca ccacgttatc accagtttgc
    19141 tgtttccagt gggcgctgaa tgccttgttg tactgttcgt acaattcgcg cgttggatca
    19201 tatgaaacgt taagaagctg aatatccttt gccataacgc tggttgccgc cagcaaaaat
    19261 gttaacccta cgccccactt gttcatcgcc cgactctctt atgttgtgtt gtgatgagca
    19321 aagcgtgcca gaaggttaac caaacattaa agaataaaaa aagattggct ataacttgcg
    19381 ggtatatgtt gagggattaa aaaggcggaa gaaatccgcc tcatattgct gacaaagtgc
    19441 gctttatcga tgccggatgc ggcgcgaacg ccttatccga cctacaaaaa tttgtaaatt
    19501 caataaattg cagaattcac gtaggcctga taagcgaagc gcatcaggca tttttgcttc
    19561 tgtcatcggt ttcaggctaa aggaatctgc ctttttccga aatcattaat acagtttttt
    19621 cgcgcagtcc agccagtcac ctttgaacgg acgcttcatg ttttcgatag cgtcgatgat
    19681 gtcgtggtga accagctgtt cgttctggat acctacacaa cgaccgccgt aacctgccag
    19741 cagcagatcg atagcgtaag cgcccatacg ggaagccaga atacggtcgt aaggcaccgg
    19801 agaaccaccg cgctggatgt ggcccagcac agttgcgcgg gtttcacgac cggtttcttt
    19861 ctcgatgaaa tgcgccagtt cgtcaacatc acacatatgt tcggtaatcg ccacgatcgc
    19921 gtgtttttta cctttcgcga tacccgcttt gatttcgttt accaggtctt cacggctgaa
    19981 ttcaacttcc ggaaccacaa cgaattcaca gccaccggca atggccgcag ccaacgtcag
    20041 atcgccacaa taacggccca tcacttccac cacggaaata cgctggtgag aagaagaggt
    20101 gtcacgcaga cggtcgatcg cttctacaac ggtgctcagc gcagtgaaga aaccgatagt
    20161 gtagtcagtg cctttgatgt cgttgtcgat agtgcccggc agaccgatgc acgggaagcc
    20221 catttcggtc agacgcattg cacccatgta ggaaccgtca ccgccgataa ccaccagcgc
    20281 gtcgatccca cgttttttca ggttttcgat agccacggcg cggatgttct cgtcgcggaa
    20341 ttccgggaag cgcgcagaac cgaggaacgt accgccacgg ttgatcatgt cagaaacgct
    20401 gtaacggtct agctgtacca tacggtcttc atacagaccc agatagccgt cataaatgcc
    20461 cattacttcc agaccttctg tcagcgcaga acgaacaacc ccgcgaattg cggcgttcat
    20521 gcctggcgca tcaccgccgc ttgtcaacac accgattttc ttaatcatga ctacctctga
    20581 actttggaat gcaaaataaa atctgttgcc ggaagtcttc ttgcacatcg aagtgatcca
    20641 acgaatgtgc aaatagtata acaatcactt cctgctgaat tgattcaggt caggccaaat
    20701 ggcggtattt tatacacaaa atgcgggtct ggctctcttt tatactgatt atgaaagcat
    20761 agaccgttta ccctccctgg gtacgacgga acaggggtcc tgatggataa ttacatccga
    20821 tcccggaaaa cgccgtaaaa tagcctgctc tacctgatcc gccaccatat gtgcctgaac
    20881 caaaggcaga gagtcttcca tttccaaatg aatctgaata aagcgggtcg gccctgactg
    20941 ccgcgtgcga agatcgtgag cgccgctaac acccggccag gaagtcacga tatcaataat
    21001 ttcttgccgt tcctcatcag gcaatgcgcg atccagtaat gactgtaccg cctcatatcc
    21061 catgcgtaac gcgctatata aaatatagat gccgattccc aatgcaaaca gagcatcggc
    21121 gcgatgccag ccgtaccagg acaaccccag cgccagcaga attgcgccgt tcatcataac
    21181 atcagactgg taatgtagca tatcagcccg caccgcctgg ctttgcgtcc ggcgcaccac
    21241 ccaacgctga aacgagacaa ggataatcgt acaaattagc gccacaattg tcacgataac
    21301 cccgacgcct ggatctgtca tcggtgttgg agatatcaga tgttgaatac ccgtcaaaaa
    21361 caggaatagt gccgaaccgg agataaacat actttgcgcc agcgccgcga gggactctgc
    21421 tttaccgtga ccaaacgagt gattatcgtc ggcaggttgc agggaatatc gcaccaccag
    21481 taaattcgtc aacgacgcgc cgatatccac cagcgaatcc accagcgcgg cgagaatact
    21541 caccgacccg gtataccacc atgcaaaaat tttaatcagc aatagcaacg aagccatcgc
    21601 cgtcgcagca atcgccgccc gactgaccag ccgtccataa gattgattca taaatactcc
    21661 cgctatcaac tgacgctagt ataacggaag caaatcatct gcaatgcatt aagcagcagg
    21721 caaattgagg ataaaaaaaa cccccacatc atgtggggga agacagggat ggtgtcacaa
    21781 aaaagcacct aacctgatga tataaatgga tttatgtcaa ccgctgtcca catagcgtcc
    21841 acatcgttca gcgtccgttt aatcggacgc ttttaaattt ttatttttcc cagcagaaaa
    21901 attagctttc ccgctgtttt gcaactcaac aagaggagat aaccgtcggt aaacagccaa
    21961 aagtatcttt aactgaacca gttcatctgc ccccgatagt tgaaccaagt ttttacgttg
    22021 ttcttcacta agtgccatca cagattcaag tgatttaact gcgtcagaaa caagttcaat
    22081 ctctttaatt tgattctgta tacgcagctt ctctgatttc aactttgccc attctctttg
    22141 tcgatatgta cgcatcacct gatcgtaaac tttgttggca gccagaatac ttccaccaac
    22201 agcagtaact aactcagcaa tctgccaaga gatatcaata agagcagaca attcaataaa
    22261 gccaggagag gcatactgaa tttttttcac tacaggacgt aaatctggtg gagtcgcact
    22321 gtaagcccct ctaaaaaagt tcaccacact gtgccccccc tcccaaggca ttgttgaata
    22381 tcctcttgat cctcctttta ggccataaaa aaaaccatat agctgcaaat actctttcgt
    22441 taagaccgcg aaatcagcta actcccaacg attatcaagc tcaattataa ctttttccat
    22501 tttccaccaa cctgtccaac tttaaagatt gacttttaag
//
LOCUS       DAPHDW010000070        20879 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.070, whole genome shotgun sequence.
ACCESSION   DAPHDW010000070 DAPHDW010000000
VERSION     DAPHDW010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20879)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20879)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20879
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.070"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            234..1130
                     /locus_tag="RH057_002635"
     CDS             234..1130
                     /locus_tag="RH057_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000785677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WYL domain-containing protein"
                     /protein_id="HDV3128986.1"
                     /translation="MVDSTRNRSAERLIDILVELQNYGVVSRYNLMKKYNITERTAYR
                     DLNMLSPFIEACGDGKYRLISARAGNQSKESLHKSLARLLDTDAIFPERDEGFWQKLE
                     NRATEKHIRVQFHNPEHTIRDDLRKYLDVLEKAICNSNVCQIAYAGKTRIVHPYKLTN
                     QRSIWYLLATEENKLKSFSLAKIKWLDIKKEKFAKSDEIQSLVSESCDPWVSDKTFDV
                     VLFIYSNIAHYFLRRDLLPYQQLLHKHDNGITLSCKAAHKNQIVPLILYWLPNVEIIE
                     PVWLKEAVLTMLGKYLTAENVS"
     gene            1179..2258
                     /locus_tag="RH057_002636"
     CDS             1179..2258
                     /locus_tag="RH057_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein"
                     /protein_id="HDV3128987.1"
                     /translation="MALPFIILGVAAGAAAFGGKKAYDGYQKKSEAEDILEKAKERYE
                     EHKEKFEQAEAQTSSKLTTLGNKELEIGKDFSEFDRIAAELLARLEKEGNKDLKLSVP
                     QHNINKIQNLAISATEYLSTVVAAGVSGAAAGFAVYSGVMAFAAASTGTPIAALSGAA
                     AYNATMAAIGGGSLAAGGMGMAGGAMVLGTAVAAPLIAIAGWAYDRHATKALENAHQC
                     ACDVARAVKKMNLAREHFAKVNQYVDEILAALNRMHAVFIKHYFEPLKSMHSVITEQQ
                     DISVSDEVIVLIDHGYSLAAIMTDIITTPLFKPRKQENGEAVIKDNVIEMETDDNGMN
                     VINKDELDDVLASSVVKFDDFSSQS"
     gene            2305..3876
                     /locus_tag="RH057_002637"
     CDS             2305..3876
                     /locus_tag="RH057_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP--cob(I)alamin adenosyltransferase"
                     /protein_id="HDV3128988.1"
                     /translation="MNVEKFDWTEELHQTMVKSLVTSFGLDFLLLNDRKGGDVDTIHN
                     VRNGIYATEAERQRYEGRGEYDSHHYHSHENYIATNREGKRAHQAGTLTDAYTGEVFA
                     SDDKKNLDHIISAKEIHDDPGRILAERDGAELANDASNLAFTHESLNKSKKADSMDAF
                     ICTLQKNREDTLRQIAELESQATLTEKEKKRLISLRKKADADTERMERADKYAREKYE
                     ATINQSYYLSSKFATAVASASLNSGFRMGTRQMLGLILAEIWFELRERIPHIIRQQRN
                     SFSAGKCLSEITSALRACQERVQEKFSSFLIAFKDGVIGGILSGITTTLLNIFLTTER
                     MIVRLIREMWNNLVQAFKVLVFNPEGLSPGQLAKAVTKLIAAGVAVAGGVMINEAMAQ
                     ILIFPFGSELAAFCGALTTGILGLVMNYYLEYSEVMQRVWAFLDKFKDKFQRSLEYFQ
                     AVNAELDRYLVELTALEFSVDAAELNHFSLHLDSVNSEIERGLLLREEVRRRGIALPF
                     ESGNVASVRDWLNKR"
     gene            3873..4139
                     /locus_tag="RH057_002638"
     CDS             3873..4139
                     /locus_tag="RH057_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000766272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV3128989.1"
                     /translation="MKLHPPFPCNQCGACCRHVNRAGETQCLDRGDGICRHYQTDSHL
                     CAIYDKRPQICRVEDQYLLNYQSQYSWQEFIALNQAACLILNKL"
     gene            4279..4476
                     /locus_tag="RH057_002639"
     CDS             4279..4476
                     /locus_tag="RH057_002639"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3128990.1"
                     /translation="MRLLLKGLVGMALAWLVIGFGMELFFLADRVHPALRVIVVLGML
                     FLLIMIAGGMCRYLLSRKKTS"
     gene            4542..6215
                     /locus_tag="RH057_002640"
     CDS             4542..6215
                     /locus_tag="RH057_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001398269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HDV3128991.1"
                     /translation="MYSSATVCFTVSAGQKYRFYSIIMKINLPFIHWSRISNDKRLVL
                     SVVVFTLSNILVSSGAHLIYRILFFTLALLIIKAMKSLTLRFILAFSFTLLTAADISI
                     SLYSWCTFGTTFNDGFAISILQTDPDEVIRMFRMYVVYVIAFIILFLLFFCSAINKTS
                     SLPLEKVTVITFLLLITVTLYSSFQFALKKQYQINEVDPYIVASRFATYTPFFNLNYF
                     ALAAKEHQRLMTIADTIPHYDLVITDNNTDVFVLVIGESARADNMSIYGYSRPTTPEL
                     QKQKSRLKLFTQAISGAPYTALAVPLALSVNTVLHHDVRHYPDNIINMANQAGFDTWW
                     LSAQSAFRQNGTAVASIAMRARNRIYVRGYDELLLPHLAEALNSNPGGRKLIVLHLTG
                     SHEPVCSNWPRDKAVFKPLDTEEVCYDNSIHYTDSLLGQVFAMLETRRASVMYFSDHG
                     LEYDPTKEHAYFHGGIKPSQQAYHVPMFIWYSPALGEHVDRQTVNSVFSTAYNDYLIN
                     AWMGVTRPAQPKTPEEVITRWQGKSVVFDASHNVFDYKVLRKNFNEPLE"
     gene            complement(6359..7402)
                     /locus_tag="RH057_002641"
     CDS             complement(6359..7402)
                     /locus_tag="RH057_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HDV3128992.1"
                     /translation="MNDRLRFEVNDNQGRFVFPDIWFGPLLGEFEELLDAYDTDEISE
                     TSYINKLRRLAQQEPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRLIPESFSG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKKHADEFSPYWYELGLLHFLNGELVKAATAFRRGFAANTYIAEIL
                     CGNLHPFPLAVWHNYSAGPDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERAE
                     IIKCAEMLMQEDDFEICESILRQQKLLRERIDETLSEEIVQKCRNINGEYVWPWILPF
                     SAAGMKHTSIQHR"
     gene            complement(7836..9026)
                     /locus_tag="RH057_002642"
     CDS             complement(7836..9026)
                     /locus_tag="RH057_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3128993.1"
                     /translation="MALTARQVETARPKEKDYKLSDERGLYLLVKTTGARYWRLKYRI
                     AGKEKKLALGVYPDVSLAEARIKRDDARKIISEGGDPGEKKRKEKLTQKISATNTFHA
                     LATEWHQHKSLSWSESYARSVLEALDKDIFPYLGKRSVTDILPLEMLEILRRIEKRGS
                     LEKLRKVRQYCNQIFRYAIATGRATVNPASELTSTLAAPKAAHFPHLRADELPVFLRK
                     LAEYHGSPVTRMATNLLLLTGLRTIELRSAEWSEIDFDNALWTIPESRMKMRRKHVVP
                     LSRQTTDILLQLKTFSGQYQLVFPGRCDINKPMSEASINMVLKRIGYDGRATGHGFRH
                     TMSTILHEQGFNSAWIEMQLAHVDKNAIRGTYNHAQYLDGRREMMQWYADYIDSLSRQ
                     ESQG"
     gene            complement(9203..9278)
                     /locus_tag="RH057_002643"
     tRNA            complement(9203..9278)
                     /locus_tag="RH057_002643"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9243..9245),aa:Phe,seq:gaa)
     gene            complement(9385..9960)
                     /locus_tag="RH057_002644"
     CDS             complement(9385..9960)
                     /locus_tag="RH057_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3128994.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
     gene            complement(9997..11694)
                     /gene="dsbD"
                     /locus_tag="RH057_002645"
     CDS             complement(9997..11694)
                     /gene="dsbD"
                     /locus_tag="RH057_002645"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HDV3128995.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFTFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADVQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIMLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPGGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWLGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            complement(11670..12008)
                     /gene="cutA"
                     /locus_tag="RH057_002646"
     CDS             complement(11670..12008)
                     /gene="cutA"
                     /locus_tag="RH057_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HDV3128996.1"
                     /translation="MLDEKSSNTASVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            complement(12124..13425)
                     /gene="dcuA"
                     /locus_tag="RH057_002647"
     CDS             complement(12124..13425)
                     /gene="dcuA"
                     /locus_tag="RH057_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HDV3128997.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            complement(13543..14979)
                     /gene="aspA"
                     /locus_tag="RH057_002648"
     CDS             complement(13543..14979)
                     /gene="aspA"
                     /locus_tag="RH057_002648"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HDV3128998.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            15316..15792
                     /gene="fxsA"
                     /locus_tag="RH057_002649"
     CDS             15316..15792
                     /gene="fxsA"
                     /locus_tag="RH057_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418564.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HDV3128999.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDERDRLDHKDDRQD"
     gene            complement(15808..17064)
                     /gene="yjeH"
                     /locus_tag="RH057_002650"
     CDS             complement(15808..17064)
                     /gene="yjeH"
                     /locus_tag="RH057_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HDV3129000.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTRGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPANIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEKMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLAGLWLLLPKRKTPENGITT"
     gene            17340..17633
                     /locus_tag="RH057_002651"
     CDS             17340..17633
                     /locus_tag="RH057_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HDV3129001.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            17677..19323
                     /gene="groL"
                     /locus_tag="RH057_002652"
     CDS             17677..19323
                     /gene="groL"
                     /locus_tag="RH057_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HDV3129002.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            19461..19814
                     /locus_tag="RH057_002653"
     CDS             19461..19814
                     /locus_tag="RH057_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HDV3129003.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(20007..20876)
                     /locus_tag="RH057_002654"
     CDS             complement(20007..20876)
                     /locus_tag="RH057_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HDV3129004.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRMHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQENGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT         5502 a         4751 c         5256 g         5370 t
ORIGIN      
        1 ctgttaaatg aaaaaggact gttgttggga cacgttgttt tttgtcatat cagcccttcc
       61 ggaaacaata aaccgtatta cgaaggaggc tcccggacat ctggtttaaa gtttgaacat
      121 aatcaaataa aaatctgttg ccggagggcg tagttttgtg gaactgacaa atgatgtcag
      181 gagtatttat ataattcact tacacaagag taatactaca ggtgcagtga tttatggttg
      241 actcaacgag aaatcgctca gcggagcgtt tgattgatat ccttgttgaa ttacaaaatt
      301 atggcgtggt cagtcgctat aatttgatga aaaaatacaa catcactgag cgaacagcat
      361 atcgtgatct gaatatgctt tctcccttta ttgaagcctg tggcgatgga aaataccggc
      421 ttatttcagc aagagcagga aaccagagca aagaatcact gcacaaatct ctggccaggt
      481 tactggatac tgatgcgatt tttcctgaac gggatgaagg tttctggcag aaattagaaa
      541 acagggcaac cgaaaagcat attcgtgttc agtttcataa ccctgagcac actatcaggg
      601 atgatttaag aaaatatctt gatgttcttg aaaaagcgat ctgcaacagt aatgtttgcc
      661 agattgcata tgccggtaag accagaatcg ttcatcccta taaattaact aatcagagaa
      721 gtatatggta tttgctggct actgaagaga acaagttaaa gtcgttttca ttagctaaaa
      781 taaaatggct ggatataaaa aaagaaaagt ttgcaaagag tgatgaaata cagtcactag
      841 taagtgagtc atgtgacccg tgggtgagcg acaaaacatt tgatgttgtg ttgttcattt
      901 acagtaatat cgcccattat tttttgcgac gggacttact tccttaccag caattgctac
      961 acaaacatga caatggcatt acgctgtcat gcaaggctgc acataaaaat caaattgtcc
     1021 cgttgatttt atactggcta ccgaatgttg aaattattga accagtatgg ctgaaagaag
     1081 ccgtgttaac aatgttagga aaatacctta ctgctgagaa tgtttcctga aatatttcag
     1141 ttgactgatg ataaagcaat ctgtacagga ggaaaagaat ggcgttacct tttattattt
     1201 tgggtgtggc tgccggcgct gcggcattcg gcggaaaaaa agcttacgat ggatatcaaa
     1261 aaaagtcaga agcagaggat atcctcgaaa aagcaaaaga gcgttacgag gagcataaag
     1321 aaaaatttga gcaggctgaa gctcagacat catcaaaatt aacaacgctc ggaaataaag
     1381 agcttgagat tggtaaagat ttcagtgagt tcgacaggat tgctgctgag ctcctggcgc
     1441 gtctggaaaa agagggcaat aaagacctga aactgagtgt cccgcaacat aacatcaata
     1501 agattcagaa cctggccatt tcagcgacag aatatctcag tacggtcgtt gctgccggag
     1561 tttccggggc tgctgcgggt tttgctgtgt atagcggagt gatggcattt gctgcggctt
     1621 ctacaggtac acccattgca gcgctttctg gtgccgcggc ttataacgcg acgatggcgg
     1681 caattggagg gggctctctc gcggcagggg gcatgggaat ggccggcggc gcaatggtat
     1741 taggcaccgc agtggctgca ccgttgattg ccattgcggg ctgggcttac gacagacatg
     1801 cgacgaaagc tcttgaaaat gcccatcagt gtgcctgcga tgttgcacgg gcagttaaga
     1861 aaatgaatct ggcccgggag cattttgcaa aagttaatca atacgttgat gaaattctcg
     1921 ctgcactgaa cagaatgcat gctgtattta ttaaacacta ttttgagccg cttaaatcca
     1981 tgcattctgt tattacagaa caacaggata tcagtgtatc agatgaagtc attgtgctta
     2041 ttgaccatgg atattcactg gccgctatta tgacggatat tattacaacg ccactgttta
     2101 aaccgcggaa acaggaaaat ggtgaggcag tgataaaaga taacgttatt gaaatggaaa
     2161 cggatgataa tggaatgaac gttattaata aggatgagct ggatgatgta ctggccagtt
     2221 cggttgtgaa atttgatgat ttttcatccc agagttaatg atttaccggg cgttaaagcc
     2281 cggctttaca agaagtgaaa taacatgaat gtggaaaaat ttgactggac agaagagtta
     2341 catcagacca tggtgaagag cctggttaca tcattcgggc tggattttct gttgctaaat
     2401 gatcgtaagg gtggtgatgt tgacactatc cataatgtgc gtaatggcat ttatgccacg
     2461 gaggctgagc gacagcggta tgaaggtcgt ggcgaatatg attctcatca ttaccacagc
     2521 catgagaact atatcgcgac gaaccgggaa ggaaagcgtg cgcatcaggc agggactctt
     2581 actgatgcat atacaggcga ggtgtttgct tctgacgaca aaaaaaatct ggatcatatt
     2641 atttccgcta aagaaattca tgatgatccg gggagaattc ttgctgaacg ggatggtgca
     2701 gaactggcaa atgacgcttc aaatctggca ttcactcatg agtcgctcaa taaatcaaag
     2761 aaagcagact caatggatgc gtttatttgc acgctgcaga aaaacagaga agatacgtta
     2821 aggcagatag cagagcttga atctcaggca acgctgacag aaaaggaaaa gaaacgtctg
     2881 ataagtctga gaaaaaaagc tgatgcagat actgagcgta tggaaagggc tgataaatat
     2941 gcccgggaaa aatatgaggc cacgattaat cagagctatt acctgagtag taagtttgct
     3001 actgctgtcg ccagtgcttc gcttaattcc ggttttcgta tggggacgcg acagatgttg
     3061 gggcttattc ttgccgagat ctggttcgaa ctgagggaac gaatcccgca tattatcagg
     3121 cagcaacgga actcattcag cgccggcaaa tgcctgtccg aaataacgtc agcattacgt
     3181 gcctgccagg aacgagtaca ggaaaaattc agttcttttt taattgcctt taaagatggt
     3241 gtcatcggag ggatactgtc aggtattacc acaacgctgt taaatatatt cctgacaacg
     3301 gaaagaatga ttgtccggct tatcagggaa atgtggaaca atctcgttca ggcctttaaa
     3361 gtcctggtat ttaatccgga gggattatcg ccggggcagt tagccaaagc ggtcactaaa
     3421 ctgatagcag caggtgtggc tgttgccgga ggggtgatga taaatgaggc gatggcgcag
     3481 atccttattt tcccatttgg ttcagagctt gctgcctttt gtggtgcgct gacgacaggt
     3541 attctcgggc tggtgatgaa ttattatctc gaatatagcg aggttatgca gcgggtatgg
     3601 gcatttctgg acaaatttaa ggacaaattc cagcggtcac tggaatactt tcaggcagtc
     3661 aatgctgaac ttgatcgcta tcttgtggaa cttactgcac tggaattttc tgttgatgct
     3721 gccgagttaa atcatttttc acttcatctg gatagcgtaa acagtgagat agagcgcgga
     3781 cttctgctga gagaagaagt ccgccgacga gggattgcgt tgccgtttga gtcaggaaat
     3841 gtggcttctg ttcgtgactg gttaaataag cgatgaaatt acaccctcca tttccctgta
     3901 accagtgtgg ggcctgttgt cgtcatgtca acagggcggg tgaaacgcaa tgtctggatc
     3961 gtggtgacgg tatttgccgt cactatcaga ctgattctca tttgtgtgca atttatgata
     4021 aacgcccaca gatttgccgg gttgaagacc agtatttact gaattatcag tctcagtaca
     4081 gctggcagga gtttattgca ttaaaccagg ccgcatgcct tattctgaat aaactgtaat
     4141 gccattaatg gcggcgtcag gacagaaact gagagaacat aaaacagtaa aatgtcatac
     4201 cattcagcag ccagcgttac tttccccggc tgctgttatt gtaaggcatt ttcgttaaga
     4261 atacagaagg agaggagcgt gagattgctg ttaaaagggc tcgtcggaat ggcgctggcc
     4321 tggcttgtta ttggtttcgg gatggagtta ttttttctcg ccgatcgggt tcatccggca
     4381 ttaagagtaa ttgttgtcct ggggatgtta ttcctgctga taatgattgc gggtggtatg
     4441 tgccgttatc ttctgtcacg aaagaaaaca tcatgacgat cctgtttacg ccgctacatt
     4501 tgtggcggcg gatgatgccc gttttatgat tattcagaaa aatgtattca tctgccacgg
     4561 tatgtttcac ggtcagcgcc gggcagaaat atcgctttta ctcaattatt atgaaaatta
     4621 atcttccgtt catacactgg tcacggatta gcaatgataa gcgcctcgtg ttatcagtgg
     4681 ttgtctttac cctttctaat atccttgtat cctcaggagc gcatctgatt taccggatat
     4741 tattcttcac cctggcgctt cttattatta aggcgatgaa atcgcttacg ctgcgtttta
     4801 ttcttgcctt ttcgttcaca ttgctgacag cggcagatat cagtatcagt ttgtattcat
     4861 ggtgtacatt cggtacaaca tttaatgatg gttttgcaat cagtatcctt cagacagatc
     4921 ctgatgaagt aataagaatg ttcaggatgt atgttgttta tgttatcgca tttattatcc
     4981 tctttttatt gttcttctgt tcagcaataa ataaaacgtc ctcattgcca ttggaaaagg
     5041 tcacggtaat tacattttta ttgttaatta cagtaacgtt atattcttcc ttccagtttg
     5101 cgttgaagaa acagtatcaa ataaatgaag ttgatcctta catagtcgct tcccggtttg
     5161 ccacatatac ccctttcttt aatctgaact attttgcact ggccgcaaag gagcatcaga
     5221 gattaatgac gattgctgat accatacctc actacgatct ggtgattacg gataacaaca
     5281 ctgacgtatt tgttctggtg attggtgaat ctgcaagggc tgataacatg tccatttacg
     5341 ggtattcgcg cccaaccaca ccagagcttc agaaacagaa atcacgactg aaactgttca
     5401 ctcaggcaat cagcggggcg ccttataccg cccttgcggt accgctggca ttatctgtaa
     5461 ataccgtgct gcatcatgat gttcgccatt atcccgataa tattatcaac atggcgaatc
     5521 aggcgggatt cgatacctgg tggctcagcg cacaatcggc gttccggcag aacggaacag
     5581 ctgtggccag tattgccatg cgggccagaa acagaattta tgtcagaggc tatgatgagt
     5641 tactgctccc acacctggct gaagcactga acagtaatcc gggaggcagg aaactgattg
     5701 ttctgcatct gacgggcagc catgaaccgg tttgcagtaa ctggcccaga gataaagccg
     5761 tgtttaaacc gctggataca gaagaagtct gttacgataa ttccattcat tataccgaca
     5821 gtttgttggg gcaggttttc gccatgctgg aaacgcgccg ggcatccgtc atgtattttt
     5881 cagatcacgg tctggagtat gacccgacaa aagaacatgc ctattttcat ggtggaataa
     5941 aacccagcca gcaggcttat catgttccga tgtttatctg gtacagcccg gcgctgggag
     6001 agcatgtcga tcgccagacg gtgaacagcg ttttttccac tgcgtataat gattatctga
     6061 ttaatgcatg gatgggcgta accagaccgg ctcaaccgaa aacgccggag gaggtgatta
     6121 cccgctggca gggaaaatcc gtggtttttg atgccagcca taacgtgttt gactacaaag
     6181 tattacgaaa gaactttaat gaacccctgg aataattcgt taaagtaatt tactacgcta
     6241 ttttttcaaa ggaaactttc agaaaataac cccagggcga taacctgggg aagtgggggg
     6301 ctgtgttatt actggtggat aatttattac ttgtttgaaa gatacactaa tgcaatatct
     6361 accgatgctg gatgcttgtg tgtttcattc ctgccgctga aaacggcaat atccagggcc
     6421 agacatattc accgttaata tttctgcatt tctgtacaat ttcctcagaa agtgtctcat
     6481 caattctttc ccgcaacagc ttctgctgtc ttaaaatgct ttcgcatatt tcgaaatcat
     6541 cttcctgcat cagcatttcg gcgcatttaa tgatttctgc ccgttcatgc agcacagagg
     6601 aatggttata gagccagttt acaaacagca atgcttccgg atattgtccc cataatggtg
     6661 agtatgttgc ataataatct tcggcagtat ccggcccggc agaatagtta tgccatacag
     6721 caagaggaaa tggatgcaga tttccgcaca gaatttctgc gatgtaagta tttgccgcaa
     6781 atccgcgacg aaatgcagtt gcagctttta ccagttcccc attaagaaaa tgcagaagac
     6841 caagttcata ccagtaaggt gaaaattcat cagcatgttt tttcagtaca ctgaaggctc
     6901 gctcatgatc acctgttcgt aacagctctg aacccaacaa ccagcgggca ccctgattat
     6961 cttcaggatt gtatgccagt attttatcag taagcataac cgcatcctga tgacgctgta
     7021 agtgcacatt tgcgagaatt gctgcataaa gcgcgcgcag ataaggccgg ttttcgggat
     7081 gcatccagat gatttcacca ctgaaacttt ccgggatgag acgattacct gccgccagtc
     7141 ctttaagagc ggcgttcagt gccttacgcg gggcattctg ctccagaaat gcatatgcca
     7201 gatgagcgtg tatatcaata aagtcaggct cctgttgagc aaggcgtctg agtttgttga
     7261 tgtaactcgt ctcgctgatt tcatcagtgt cgtatgcatc gagcagttct tcaaactccc
     7321 ccagtaatgg gccaaaccag atatccggaa atacgaaacg cccctggtta tcgttcactt
     7381 caaagcgtaa tctgtcgttc attttctgag cctgtaaaac ggatgggaca caatctgaaa
     7441 agcataaaag ttgtgatgtt gtgtatcaag tcttgttttt gtccgggatg aatgttgttt
     7501 acaatatcat cggcagtggt ttttcatgag aatgaaaatt tatctttatc tgcattccgg
     7561 tgtattgtga attgtcggct gaaacagcat attaatcgat ttttttaggg gaatggtgct
     7621 gaaataacga catgatgatg atttatatta caggtatcaa aattgttagc ggtccataac
     7681 ggtgtgatgt tttgagattg tttacagatg caatattaaa tattttgtca aaaaacaaaa
     7741 aatatctaaa aataaagcat gaaaacaaaa ttcctccgga cctggaggcc ggaggagaac
     7801 ttgtggattg agagaaaata tcagaacaag tgttcttaac cctgactctc ttgcctggaa
     7861 agcgaatcaa tgtaatctgc gtaccattgc atcatttcac ggcgaccatc gagatattgg
     7921 gcatgattat aggtacccct gatggcgttt ttatccacat gagctaactg catttcaatc
     7981 caggcagaat taaagccctg ttcgtgcaga atggtactca tggtgtgacg aaaaccatga
     8041 ccggttgccc tgccatcgta accgatacgt ttgagcacca tgttgatgct ggcttcgctc
     8101 attggcttgt tgatatcaca acgtcccgga aaaaccagct ggtattgtcc ggagaaagtt
     8161 ttgagctgca gcagaatgtc agtggtctgt cgtgacagtg gtacgacatg tttacgtcgc
     8221 attttcatgc ggctttcagg gattgtccac agggcattat caaaatcaat ttctgaccat
     8281 tcagcggacc gtagttcaat cgttctgagg cctgtcagaa gcagcagatt tgtcgccatg
     8341 cgggtaacag gactgccatg atattcagcg agcttccgga gaaaaacagg gagctcatct
     8401 gctctcaggt gggggaaatg tgcagctttt ggtgccgcca gcgtactggt cagttcagat
     8461 gccggattga cagtggctcg tccggtggcg atggcataac gaaaaatctg attacagtat
     8521 tggcgtacct tacgcagttt ctccagcgag ccacgttttt ctatgcggcg cagaatttcc
     8581 agcatttcca gcgggaggat atccgtaacg cttcgtttgc ccagatacgg gaaaatatct
     8641 ttatccagcg cttccagtac gcttctggcg taactttcag accatgacaa agatttatgc
     8701 tggtgccatt ccgtagcgag ggcatggaac gtattggtgg cagagatttt ctgagtgagt
     8761 ttttcctttc gcttcttttc gcccgggtca ccaccttcgg agataatttt tcgggcatcg
     8821 tcgcgtttga ttctggcttc agcaagggag acgtcgggat agacgccgag ggccagtttt
     8881 ttctcttttc ctgctatccg gtatttaagc cgccagtagc gggcacccgt ggtttttacc
     8941 agcagatata aaccacgttc gtcagagagt ttatagtctt tttctttagg tctggctgtt
     9001 tcgacctgtc tggctgtaag tgccatatgg gggctcctga aactgaccga acacaatgag
     9061 cccccaccgg tgcccccaat gaacgcttga ttgagagaga gttcagtaga gttcggtgga
     9121 gttcaggatg ttcagattgc ttgtaacgat gtgattatac ttgagtttcg tggagttcgg
     9181 gagatgaatc tggaagtgaa agtggtgccc ggactcggaa tcgaaccaag gacacgggga
     9241 ttttcaatcc cctgctctac cgactgagct atccgggcaa cggggcgcat taaaccgtaa
     9301 tctgcacatc tcgtcaacct aatttcagta aaagcgattc aactgcttaa gattgcggca
     9361 aatcgcttct tttctgtgtt tctgtcaggt caatgcacca ccctggcggc agcgggcgaa
     9421 gcgcaggata tcttccgcca gccgatgtgc agtgtcgaca tccgcctggc tacgattcac
     9481 cagcattcgg cttaaacagc cttccagcac cagttccatc tgctttgcta ccatcgccgg
     9541 atcgtcaact tccagtgtgg ttaacagttc gtgggtgaaa tcgtaggccg cgcttttttg
     9601 ctgatcggcc agttgatgaa tagggtggcc aggatcggga taaaacgtac aggcagcgat
     9661 aaatagacag cccggatagc ggttgttttt aacgcactcc gataacgcct gataacgtgc
     9721 cagcagcttt tgttcggcgg tttgcgtttc gtccagcatc agctgacgac gccagacatc
     9781 tatctgttgg ctaagataac gcagcgcatc gtagaggatt gcctctttgt ctggccagaa
     9841 gcggcgtagc tcgtccagtg gataatccac acgttcagca accatctcca gcgtggtgtt
     9901 ggcaatccct tgtaattcta ataatttcag ggcttctccc agtacatctt cacgttgcac
     9961 gctattttcc tccgtctttc ccactgcaag tgtcgttcac ggttggcgat cgcgcaaatg
    10021 tgcgctgaag gtttcagcat ccataaagcc cgtgacgcgt gcttgtggat gctcctggcc
    10081 ttgtccgtca aaaaagagaa ttgtcggtag gccaaggaca ttaagatgct ttaacagcgc
    10141 cacatcttgt gcgtcgttgg ccgtgacgtt ggcctgaagt aagaccgtgt ctgctaacgc
    10201 tttttgcacc tgcgggtcgc tgaaggtgta tttctcaaac tctttacagg cgacgcacca
    10261 gtcggcataa agatctaaca tcaccggttt gcctttggct tcaacgagcg cctgatttaa
    10321 ctcatctacc gttttgattt gtgtaaagtt gagatgcgtc tgagtttgcg cggtatgcgt
    10381 cgcaccaaat gcccaatcct gaagtgggcg cacgctaacc aatgccgctg ccagcaggat
    10441 tatttgcacc acgcgcatcc agccgcgttt agcctgtagg ctggtgataa aggcccagcc
    10501 aaagaatgcg acacccagcg ccgaccacaa gcgtaatccc catacatcac caatcactcg
    10561 ctccagcagg aagaccggca gtgcgaggat cacaaaacca aacgcggttt tgacttgttc
    10621 catccacggg ccgcttttcg gcagcaagcg gttaccaaag acggtaatta gcatcagcgg
    10681 caggcccatg cccaacgcat agagataaag cgtgccgccg cccagccaca tgttcccgct
    10741 ttgggcgata tacagcagaa tcgcgctaag cggtgcggtg gtgcatggtg aacagatcag
    10801 tccggcaatc gcccccataa caaacacacc gccaggtgag ccgccctgtt ggcgattgct
    10861 catcaacgtg agacgtgttt gcagcgaaga ggggagttgt agagtaaaca agccaaacat
    10921 tgacatcgcc agcaaggtaa agacgatggc gaggccaatg agcacgtatg ggtgctgtag
    10981 cgccgcctgg aactgtaacc ctgcggcggc aactaccaga cccagcgccg tgtaagtcag
    11041 tgccatcccc tgcacataaa taaaggtcag caacaatgct ctggcagtgg agagccgctg
    11101 tttaccaccc agcatgatgc cagaaatcag tgggtacatt ggcagcacgc atggcgtaaa
    11161 ggcgatacca ataccgatca acaacgccca gagcgcggaa aagggcaatt gcgcggtgtg
    11221 ctgctcttgc tgcgaaacag acacaggctg tgacgctgcg ttgttggcga ccacttcgct
    11281 taacggaacg gttttggttt ctggcggata acagaaaccg gcatcagcac agccctggta
    11341 ggtgacagtt aacgtcgctc ccgcactcgc ctggttgatg gttacgggaa gcgtcagccg
    11401 atcgcggtaa atctcgcttt tgccgtaaaa ctcatcttca tgccagacgc cttgcggcag
    11461 ctgcacgtcg gcaattttcg cgtgttccgg cgtaatgcgg atctgtttac ggtagaggta
    11521 gtaaccgtct ttgatctgcc aggtcagatt aaggtcatgt tggttttgct gaaaatcaaa
    11581 agtaaaggct tgatccgcgg ggacaaattg tgaacgtccc ggcgcgtcga ataatccggc
    11641 aaaaacggaa gtgctgcaaa gtagcaggat cagcgtaaag atgcgttgag ccatgagagg
    11701 taatctgtgt ctccgtgtgt aacaggtaaa accagaagtt ccggggtttg atatggatga
    11761 tgagacttca ggcattccag cagtgcctgc tggtgagata cggtagtttt taaaatcatc
    11821 tgcacttcgt attcttgctc cagcttacct tcccagtaat agagagaggt agcgccgggg
    11881 atcaaggtcg cgcaggccgc cagtttttcc gccagcactt tggcggctaa atcctgggct
    11941 gtcgcttcat ccggtgcggt acatagcacc acgacagacg cggtattcga acttttttca
    12001 tcaagcataa acacctcgca agaacagatg gaaccgcaag agagaggtca ctatacaacg
    12061 gacgggggaa ggatgttagt cggcgggaaa gaaagcgggg cgtgaacgcc ccgcgattgg
    12121 tcattacagc atgaagctac ccagcacgaa gccgaagcaa acggccaggg caacacccag
    12181 agtacccggg atgaagaacg gatggttgaa gacgaattta ccgatacggg ttgtacccgt
    12241 gtcatccatc tgtaccgcag caaccagcgt cgggtaggtc ggcagaatga acagaccaga
    12301 caccgcagcg aaagaagcaa cagcggtcag cggtgaaacg ttcagtgcca gggccatcgg
    12361 catcagtgct tttgcggttg cagcctgaga gtacagcaga gcagaagcaa agaagaagat
    12421 gacggccagc agccacggat gaccctgaat cacttcacca gcggtatctt tgatccagtc
    12481 gatgttgttg gaaacgaaag tatcgcccag ccacgcaaca cccaggatac aaatacaggc
    12541 gctcatacct gctttgaagg tgctggagtt gaggatgttg tcggtatcga ctttacagat
    12601 aacggtggtc agagttgcaa cgctgagcat gatgatcagg attgcgttgg tggtgttcat
    12661 cagcggtttt tcaaccagac ccatgcttgg gctgttgatg attgcataga taaccacgcc
    12721 aactacgccc agcaggaaca gccaaacgga cgtttttgca ccggatttga tttcaatctg
    12781 cttttcaccg cgcagttcaa ccaggccctc ttccagacgc ttgcgataaa tcggatcgtc
    12841 agagagtttg gagttgaaca gcatagtgac cagaaaggac atcaccagaa ccgccagcag
    12901 ggtggacggg atgaccacgg agagcagatg gaggtagctg atgccatgac cttccatcac
    12961 ggaagacatg taaaccactg ccgctgagat tggcgatgcg gtgatcgcaa tctgcgcgga
    13021 taccactgca gtagacagcg gacggcaagg tttaacgcct tgttccttcg caacttcagc
    13081 gataactggc agtgtcgcca gagagatgtt gccagtacca gcaaagattg tcaggaaata
    13141 ggtcacgata ggtgcgagga tcgtgatgta tttcgggtta cggcgcagca gcttttctgt
    13201 ctgatgaacc agatagtcca gaccgccagc aacctgcatg gcagaaatag cggcgataac
    13261 cgccatgata atggaaatga catcgaacgg gatgttaccg ggtttaacgc caatagcggc
    13321 aagaaccagc acccccaatc cgcctgcaaa accaatacct attcccccca atctggcgcc
    13381 caagaagatc gccagcaaaa ctatgatgag ttctacaact agcatattag ccttccttgt
    13441 tttttaacaa gttgatatta gattgttgtt tttaagttac tgctcacaag aaaaaaggca
    13501 cgtcatctga cgtgcctttt ttatttgtac taccctgtac gattactgtt cgctttcatc
    13561 agtatagcgt tttgctttgt aagccgggtg catcagattc tgtacggaga aaatatcgtc
    13621 aagttccgct tcagtcaaca gaccgcgttc cagaacgact tcacgtacac tcttaccggt
    13681 ttcggcacag attttaccca cgatgtcacc gttgtggtga ccgatgaacg ggttcaggta
    13741 agtaacgata ccgatagagt tgtaaacgta accttcgcac acttctttgt tagcagtgat
    13801 gccgttaatg catttttcca gcaggttgta gcaagcgttg gtcagaatgt gaacggattc
    13861 gaacatggcc tggccaatga ccggctccat aacgttcaac tgcagctgac ctgcttctgc
    13921 tgccatggta acagtggtgt cgttaccgat gactttgaag catacctggt taaccacttc
    13981 cggaacaacc gggtttactt tagctggcat gatggaagag cccgcctgca gttccggcag
    14041 gttgatctcg ttcaggccgg cacgtggacc agaagagagc aagcgcaggt cgttacagat
    14101 tttggacatc ttcacagcca ggcgtttcag cgcgccgtga accataacat aagcgccgca
    14161 gtcagaggtc gcttcgatca ggtcttcagc cggtacgcat gggaagccag tgacttcagc
    14221 cagttttttc actgccagcg gagagtactc tttcggcgtg ttcagaccag taccgattgc
    14281 tgttgcgcca aggttaactt ccagcagcag ttcagcggta cgttggatat ttttcacttc
    14341 ttctttcagc aggatgctga aagcgcggaa ttcctgaccg agggtcatcg gtactgcgtc
    14401 ctgcagctgg gtacgaccca ttttcaggat gtcctggaat tcgacagctt tacgttcaaa
    14461 gccttcacgc agttggttaa tcgcatctac cagcttaatc agggaagagt aaactgcgat
    14521 acggaaaccg gtcgggtagg cgtcgttagt ggactgacat ttgttaacat ggtcgttcgg
    14581 gttcaggtac tgatattcac ctttctggtg acccatcagt tccagaccga tattggccag
    14641 cacttcgttg gtgttcatgt ttacggaagt acctgcgccg ccctggtaga cgtctaccgg
    14701 gaactgatcc atgcattttc cgttgttcag gacttcatca catgcggcaa tgatggcatt
    14761 cgctacactt ttaggaatgg tttgcagctc tttgtttgcc atagctgcgg cttttttaac
    14821 cattaccata ccgcgaacaa attcaggaat atcactgatt ttgttgttgc tgatatagaa
    14881 gttttcaatc gctctcagag tgtgaacacc atagtaggca tcagctggaa cttccctggt
    14941 acccaacaga tcttcttcga tacgaatgtt gtttgacatg tgaaccttct ttttcaagct
    15001 gccaatgatt tgctttaaac acacagaata tatgtggttt cgaatgtttt tcgaccgacg
    15061 attatcccct gcatcgaccg aatacccgag atcatatgct gcttgaggat ttctaccgta
    15121 atctggatca ctttaagtgt cggtttttac cccttaatta ttaatttgtg aaatagatca
    15181 ccgctttggg attactacca aaaatagttg cgcaaacatc ttgaaatttt gctaatgacc
    15241 acaatataag ctgaacgcga ttcgcaaccc attcaggtag ccgggtttta ccggctgcta
    15301 ttacaggaga aacctttgcg ctggttacct tttattgcca ttttccttta tgtctatatt
    15361 gagatttcaa tctttattca ggttgcccat gtattggggg tattgctgac cctcgtgctg
    15421 gttatattca cgtcagttat cgggatgtca ctggtacgta accagggctt taagaatttc
    15481 gtgctgatgc agcaaaaaat ggcggcgggt gaaaacccag cggcggagat gattaaaagt
    15541 gtttcgctga tcattgccgg tttgctgctt ttattaccgg gctttttcac cgacttcctc
    15601 ggtcttctgc ttttattacc gccggtgcaa aagcatctga cagtgaagtt gatgccgcat
    15661 ttgcgctttt ctcgtatgcc aggcggcggt tttagcgccg ggaccggtgg cggtaatact
    15721 tttgatggtg agtaccagcg aaaggatgat gagcgcgacc gccttgatca taaagacgat
    15781 cgccaggatt aatgtcgaaa cgccggatta tgtggttatg ccattttccg gcgtttttcg
    15841 ttttggcagc aacagccata accccgccag catgatcagc gcatagagac ttttccagcc
    15901 gaccattgcc agtaacagaa cgcataacag cccgccaacc accgccagta gtcgataacg
    15961 tccttgcaat aatttacagc ctgccagcat gcataacaga taaatcataa taaagatgcc
    16021 attggcataa ataataagag cgtccagatt gatctctaaa gcatgaatca ccaacgtgct
    16081 caccacacag cagccgagca ccgcattgag ggcattattc gggatatggc gagaagagag
    16141 gcgtgccagg tagtggtcag gattatgttg cgcctgcgac cagaccaggc gggcgaagct
    16201 ctgtatataa atgttgagac tggcaaagca ggccagatag ccaatcacgc aggcaatcca
    16261 taacgctcct acaccgaaca actgcactac aatttttgga agcgatgctg ccgccgccat
    16321 tttttcacca taggcgtcga agtgtaagac gactaccgta cagccccagt agactaatcc
    16381 tgccagcagc agaccaatca tcaatgcgcg aggaaaatca cgctctggat ttttaaattc
    16441 cgaggcaaga tgggcaaatg cttccagacc gacaaaacac cagaacatca ctgataacgc
    16501 agcaaataac ccggtaagtt cgatattacc aggtgccggg aaggggatat tcgcaggttt
    16561 gatatcgccc gcccaccaga tagcgacaat cagcgcgacg ataagtccgg caataacggt
    16621 ttgtagatta gcactggaac tggcaccgcg agtaccgata taccacacca gcgccagcgt
    16681 accgagttct gccaacaaca gttgccagct atgccagcca aacattgcct ggccgaaccc
    16741 ggcggcaatt tgtagcgcgg caggcaaacc cacgggaatg accgataaaa acaaccagcc
    16801 ggtgactcgc tcaagtcgcg aaccaaacgc cataccgacg aagtgcgcga caccgcctgc
    16861 gctgggatag tggcgaccca gaatcgcaaa cacaatcgca atcgggaaca ctaagataat
    16921 caaaacgggc cacgcccaca ggctgttatt gcccgctacc agcgcagcta acgcaggaac
    16981 ggcaaacacg ccagtgccta ataatgacgt cgatagcagg ccaatgcctt gggccagccc
    17041 cagttcttgt ttgagtccac tcatgggttg atatccgatt gcgcccaaat tttgggcaac
    17101 tgcgtagatt ttcgatggta gcacaatcag attcgcttat gacggcgatg aagaaattgc
    17161 gatgaaatgt gaggtgaatc agggttttca cccgattttg tgctgatcag aatttttttt
    17221 ctttttcccc cttgaagggg cgaatcctca tccccatttc tctggtcacc agccgggaaa
    17281 ccacgtaagc tccggcgtca cccataacag atacggactt tctcaaagga gagttatcaa
    17341 tgaatattcg tccattgcat gatcgcgtga tcgtcaagcg taaagaagtt gaaactaaat
    17401 ctgctggcgg catcgttctg accggctctg cagcggctaa atccacccgc ggcgaagtgc
    17461 tggctgtcgg caatggccgt atccttgaaa atggcgaagt gaagccgctg gatgtgaaag
    17521 ttggcgacat cgttattttc aacgatggct acggtgtgaa atctgagaag atcgacaatg
    17581 aagaagtgtt gatcatgtcc gaaagcgaca ttctggcaat tgttgaagcg taatcctcgc
    17641 acgacactga acatacgaat ttaaggaata aagataatgg cagctaaaga cgtaaaattc
    17701 ggtaacgacg ctcgtgtgaa aatgctgcgc ggcgtaaacg tactggcaga tgcagtgaaa
    17761 gttaccctcg gtccgaaagg ccgtaacgta gttctggata aatctttcgg tgcaccgacc
    17821 atcaccaaag atggtgtttc cgttgctcgt gaaatcgaac tggaagacaa gttcgaaaat
    17881 atgggtgcgc agatggtgaa agaagttgcc tctaaagcga acgacgctgc aggcgacggt
    17941 accaccactg caactgtact ggctcaggct atcatcactg agggtctgaa agctgttgct
    18001 gcgggcatga acccgatgga cctgaaacgt ggtatcgaca aagccgttac cgctgcagtt
    18061 gaagaactga aagcgctgtc cgtaccgtgc tctgactcta aagcgattgc tcaggttggt
    18121 accatctccg ctaactccga cgaaaccgta ggtaaactga tcgctgaagc gatggacaaa
    18181 gtcggtaaag aaggcgttat caccgttgaa gacggtaccg gtctgcagga cgaactggac
    18241 gtggttgaag gtatgcagtt cgaccgtggc tacctgtctc cttacttcat caacaagccg
    18301 gaaactggcg cagtagaact ggaaagcccg ttcatcctgc tggctgacaa gaaaatctct
    18361 aacatccgcg aaatgctgcc ggttctggaa gctgttgcca aagcaggcaa accgctgctg
    18421 atcatcgctg aagatgttga aggcgaagcg ctggcaactc tggttgttaa caccatgcgt
    18481 ggcatcgtga aagttgctgc ggttaaagct ccgggcttcg gcgatcgtcg taaagctatg
    18541 ctgcaggata tcgcaaccct gactggcggt accgtaatct ctgaagagat cggtatggag
    18601 ctggaaaaag caaccctgga agacctgggt caggctaaac gtgttgtgat caacaaagac
    18661 accaccacta tcatcgatgg cgtgggtgaa gaagctgcaa tccagggccg tgttgctcag
    18721 atccgtcagc agattgaaga agcaacttct gactacgacc gtgaaaaact gcaggaacgc
    18781 gtagcgaaac tggcaggcgg cgttgcagtt atcaaagtag gtgctgctac cgaagttgaa
    18841 atgaaagaga aaaaagcacg cgttgaagat gccctgcacg cgacccgtgc tgcggtagaa
    18901 gaaggcgtgg ttgctggtgg tggtgttgcg ctgatccgcg tagcgtctaa actggctgac
    18961 ctgcgtggtc agaacgaaga ccaaaacgtg ggtatcaaag ttgcactgcg tgcaatggaa
    19021 gctccgctgc gtcagatcgt attgaactgc ggcgaagaac cgtctgttgt tgctaacact
    19081 gttaaaggcg gcgacggcaa ctacggttac aacgcagcaa ccgaagaata cggcaacatg
    19141 atcgacatgg gtatcctgga tccaaccaaa gtaacccgtt ctgctctgca gtacgcggct
    19201 tctgtggctg gcctgatgat caccaccgag tgcatggtta ccgacctgcc gaaaaacgat
    19261 gcagctgact taggcgctgc tggcggtatg ggcggcatgg gtggcatggg cggcatgatg
    19321 taattgccct gcacctcgca gaaataaaca aacccccggg cagaaatgtc tgggggtttt
    19381 tcttttggtc atctttctag tataagattc agacacggac gacgcgagtg gcgtccagct
    19441 cattgattat ggggaataac atgcacgtaa aatacttagc agggattgtc ggtgccgcgc
    19501 tactgatggc gggttgtagc tccagcaacg aattgagtgc tgccggtcag agtgtacgca
    19561 ttgtggacga gcagccaggc gcagagtgcc agctgattgg tactgcgaca ggtaagcaaa
    19621 gcaactggct ttccgggcaa cacggagaag aaggcggttc tatgcgcggc gcagcaaacg
    19681 atctgcgcaa ccaggcggct gcaatgggcg gtaacgtgat ttatggcatc agcagcccgt
    19741 cgcagggaat gttgtccagt tttgtcccga cggatagcca gattatcggt caggtttata
    19801 agtgcccgaa ctgatgtgat ggtgacaaac gtataattgc cagatgcgtt tcgcttatca
    19861 ggcctacata aggcattgca actgattgaa tgcttgagaa tttgtaggcc ggataaggcg
    19921 tttacgccgc atccggcatg accaacgatc acgttatcag tcatttcagg ctggatggct
    19981 ccagcctcgc attagcaaag atgagattat ttcgcctgtg gtgcagtttt gctggtggat
    20041 tgaacacgta agtgatgcag cgcgcgtaag cattgatcct gggttagcgg ttgggcgtta
    20101 ttcgccgcta cggtgcgaac atagacctga catggcacac ccgcgagttt tttcagtcgt
    20161 gggctgaaat caagcattcg gcgagtgatc gcctctattt ctttacccgg cgttgcttca
    20221 cgcgttttca ccactgcgcc gctaaattgt tctttgccac tttcatcttt aaagcggtaa
    20281 agcaccgcca gataatggtc gaacaggtta tgtttccatt ccggttggga ataggtgtca
    20341 tattccagat tgccattttc ctggcagtca acgtcatata acggcaggaa atctaacagt
    20401 gaggtaatac gtaatgccag ttcacgcatt tccgcattat taattgcatg aatgattgct
    20461 cgcgccagca tttcaatctg caactcattg acgactaact cacatttgct accgtcatgt
    20521 agcgttaata cgaaggtaag attttcgcct tgattatcgg taagttccag cgtgttaacc
    20581 cgacgattaa tatcggcatt tttaagctca tcaaccagaa tttctgggat gttttctgcc
    20641 attttcttaa tgactttatc gcgagcttgc tcatactgaa ggcgtgcagc agcatccagt
    20701 ttatgctttt gatgcatacg actttccagc gcaatcagca ggtcgcgcag ttccatgaca
    20761 gacataaaga acaacgattc tttattgcga ggttctttaa ttttcagcgc cagtgcaatg
    20821 aaattattac tcttgcgaat aacaccggta ttaacaccct tgatgcttat ggccatgta
//
LOCUS       DAPHDW010000071        20853 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.071, whole genome shotgun sequence.
ACCESSION   DAPHDW010000071 DAPHDW010000000
VERSION     DAPHDW010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.071"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(168..1817)
                     /gene="actP"
                     /locus_tag="RH057_002655"
     CDS             complement(168..1817)
                     /gene="actP"
                     /locus_tag="RH057_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HDV3129005.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTFADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVAGLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMMGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            complement(1814..2128)
                     /locus_tag="RH057_002656"
     CDS             complement(1814..2128)
                     /locus_tag="RH057_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HDV3129006.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            complement(2328..4286)
                     /gene="acs"
                     /locus_tag="RH057_002657"
     CDS             complement(2328..4286)
                     /gene="acs"
                     /locus_tag="RH057_002657"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HDV3129007.1"
                     /translation="MSQIHKHTIPANIADRCLINPQQYEAMYQQSINVPDTFWGEQGK
                     ILDWIKPYQKVKNTSFAPGNVSIKWYEDGTLNLAANCLDRHLQENGDRTAIIWEGDDA
                     SQSKHISYKELHRDVCRFANTLLELGIKKGDVVAIYMPMVPEAAVAMLACARIGAVHS
                     VIFGGFSPEAVAGRIIDSNSRLVITSDEGVRAGRSIPLKKNVDDALKNPNVTSVEHVV
                     VLKRTGGKIDWQEGRDLWWHDLVEQASDQHQAEEMNAEDPLFILYTSGSTGKPKGVLH
                     TTGGYLVYAALTFKYVFDYHPGDIYWCTADVGWVTGHSYLLYGPLACGATTLMFEGVP
                     NWPTPARMAQVVDKHQVNILYTAPTAIRALMAEGDKAIEGTDRSSLRILGSVGEPINP
                     EAWEWYWKKIGNEKCPVVDTWWQTETGGFMITPLPGATELKAGSATRPFFGVQPALVD
                     NEGNPLEGATEGSLVITDSWPGQARTLFGDHERFEQTYFSTFKNMYFSGDGARRDEDG
                     YYWITGRVDDVLNVSGHRLGTAEIESALVAHPKIAEAAVVGIPHNIKGQAIYAYVTLN
                     HGEEPSPELYAEVRNWVRKEIGPLATPDVLHWTDSLPKTRSGKIMRRILRKIAAGDTS
                     NLGDTSTLADPGVVEKLLEEKQAIAMPS"
     gene            4678..6114
                     /gene="nrfA"
                     /locus_tag="RH057_002658"
     CDS             4678..6114
                     /gene="nrfA"
                     /locus_tag="RH057_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HDV3129008.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHPDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            6159..6725
                     /gene="nrfB"
                     /locus_tag="RH057_002659"
     CDS             6159..6725
                     /gene="nrfB"
                     /locus_tag="RH057_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HDV3129009.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAAQASDDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQPSPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            6722..7393
                     /gene="nrfC"
                     /locus_tag="RH057_002660"
     CDS             6722..7393
                     /gene="nrfC"
                     /locus_tag="RH057_002660"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HDV3129010.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            7390..8346
                     /gene="nrfD"
                     /locus_tag="RH057_002661"
     CDS             7390..8346
                     /gene="nrfD"
                     /locus_tag="RH057_002661"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HDV3129011.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPILPVLFLFSGISSGAAVALIAMAIRQRSNPHSTEAQFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            8366..10084
                     /locus_tag="RH057_002662"
     CDS             8366..10084
                     /locus_tag="RH057_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HDV3129012.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQTDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDGACARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQREIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPVVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQAKGNYTSEKAIVALFDHQQRIGELMPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTGADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GRKRDE"
     gene            10077..10460
                     /gene="nrfF"
                     /locus_tag="RH057_002663"
     CDS             10077..10460
                     /gene="nrfF"
                     /locus_tag="RH057_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HDV3129013.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVAEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            10457..11053
                     /gene="nrfG"
                     /locus_tag="RH057_002664"
     CDS             10457..11053
                     /gene="nrfG"
                     /locus_tag="RH057_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HDV3129014.1"
                     /translation="MKEPKIPVKMLTTLTILMVFLCVGSYLLSPKWQAVHAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTALTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            11395..12708
                     /gene="gltP"
                     /locus_tag="RH057_002665"
     CDS             11395..12708
                     /gene="gltP"
                     /locus_tag="RH057_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HDV3129015.1"
                     /translation="MKSIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVPGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(12786..13475)
                     /locus_tag="RH057_002666"
     CDS             complement(12786..13475)
                     /locus_tag="RH057_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3129016.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(13569..15716)
                     /gene="fdhF"
                     /locus_tag="RH057_002667"
     CDS             complement(13569..15716)
                     /gene="fdhF"
                     /locus_tag="RH057_002667"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(15297..15299),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HDV3129017.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVSWDEALNYVAERLSAIKEKYGPDA
                     IQTTGSSRGTGNETNYVMQKFARAVIGTNNVDCCARVUHGPSVAGLHQSVGNGAMSNA
                     INEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIVCDPRKIETARIADMHIAL
                     KNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKIVEGYTPESVEDITGVSAS
                     EIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLAMLTGNLGKPHAGVNPVRG
                     QNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVESLPAHTGYRISELPHRAAH
                     GEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFMTKTASAADVILPSTSWGE
                     HEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMGYPMHYNNTQEIWDELRHL
                     CPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKFDTPNGLAQFFTCDWVAPI
                     DKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPGYAQINTEDAKRLGIEDEA
                     LVWVHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTENLSPITKTPEYKYCA
                     VRVEPIADQRAAEQYVIDEYNKLKTRLREAALA"
     gene            complement(15914..17380)
                     /gene="mdtP"
                     /locus_tag="RH057_002668"
     CDS             complement(15914..17380)
                     /gene="mdtP"
                     /locus_tag="RH057_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HDV3129018.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAERVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            complement(17377..19428)
                     /gene="mdtO"
                     /locus_tag="RH057_002669"
     CDS             complement(17377..19428)
                     /gene="mdtO"
                     /locus_tag="RH057_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HDV3129019.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIVAIYGQTFPAMLDYPEVVVRLTLWCI
                     VVGLYPTLLMTLIGVLWFPSRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREAL
                     ALQKLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKL
                     ASEINKLQHAVAEGQCWQSDWRITESEAMAARECNLENICQTLLQLGQMDPNTPPTPA
                     AKPPSMVADAFTNPDYMRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVG
                     SSYQKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERS
                     SYIGTQMVVTFALATLENVFGPVYDLVEIRDRAMGIIIGTVVSAVIYTFVWPESEART
                     LPQKLAGTLGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLD
                     SEERALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAAQFADALEKYAAGL
                     ATALSRSPQITLEETPASQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGAT
                     Q"
     gene            complement(19428..20459)
                     /gene="mdtN"
                     /locus_tag="RH057_002670"
     CDS             complement(19428..20459)
                     /gene="mdtN"
                     /locus_tag="RH057_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HDV3129020.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMATVRAPFDGR
                     VISLKTSIGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            complement(20478..20753)
                     /locus_tag="RH057_002671"
     CDS             complement(20478..20753)
                     /locus_tag="RH057_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129021.1"
                     /translation="MPTVLSRMAMQLKKTAWIIPVFMVSGCSLSPAIPVIGAYYPSWF
                     FCAIASLILMLITRRVIQRANINLAFVGIIYTALFALYAMLFWLAFF"
BASE COUNT         4709 a         5517 c         5706 g         4921 t
ORIGIN      
        1 tataattaac aagaaagaaa aggcatattc tctgcattat cattttctac gactgtcaaa
       61 aatcgctcat tttttaatga gtttatttgt ttaatattat gggaaaaggt gatgcatgct
      121 ggagaggaag agtattcccc ggtcaggcga ccggggaagg ggagagatta atgtgcgcgg
      181 ccttgctcaa cgccaaagcc ggtctgggag cggataaact gtgcacggaa cagttcacgc
      241 tcgcgcgcac cttccgctga gttatcggtt gccgagaaga accagatgcc gaggaatgcc
      301 acgctgatag agaacagcgc cgggtattca tacgggaaga tggctttttc gtgaccaagg
      361 atctgtaccc aaatcgtcgg gccgaggatc atcagtacca ctgcggtaat cagccccagc
      421 cagccaccca tcatcgcgcc acgagtggtc agtttcgacc agtacataga aagcagaatg
      481 atcgggaagt tacagctcgc tgcgatggca aacgccaggc ctaccatgaa ggcgatgttc
      541 tgattctcaa acagcacgcc gaggataatg gcaatcacac ctaaaatcag cacggtgatt
      601 ttcgataccc gcagttcttc acgttcggtc gcgccttttt tgaagacgtt agcgtacagg
      661 tcatgcgaaa ccgctgatgc gcccgccagc gtcagaccag caaccaccgc aagaatggtg
      721 gcaaaagcaa ccgctgaaat aaaaccgagg aacaggttgc cgcccactgc attcgccagg
      781 tgaacggccg ccatgttgtt accaccaatc agatggcccg ccgcgtcttt atattccgga
      841 ttcgcaccaa ccagcatgat cgcgccgaag ccgataataa aggtcagaat atagaagtag
      901 cccataaatc cggtggcgta gaacacgctc ttacgtgctt cgcgggcatc gctgactgta
      961 aagaagcgca tcagaatgtg cggcaagccc gccgtaccaa acatcagtcc cagccccaga
     1021 gagagcgcgg agatcggatc tttcaccagt ccgcccggtt tcatgatgtc gacacctttc
     1081 gggtgtaccg ccatcgcttc gctgaacaga ttgttgaagc taaagccgac gtgtttcatc
     1141 accataaagg ccataaagct ggcaccgaac agcaacagaa cggctttaat aatctgtacc
     1201 caagtggtcg ccagcatgcc gccgaacagg acgtacatca tcatcagcac gccgaccagc
     1261 accaccgcaa tgtgatagtt aaggccaaac agcagctcga tcagtttacc tgcacccacc
     1321 atctgggcga taaggtaaag cgccaccacc accagagaac cacaggccga aagaatacga
     1381 atcggccctt gtttcagacg gtaagaggcc acatcggcaa aggtgtagcg ccccaggtta
     1441 cgcagacgtt cggcaatcag gaacagaatg atcggccagc ccaccaggaa gcccagcgag
     1501 taaattaagc cgtcatagcc ggaggtaaac accagcgcgg agatccccaa aaatgaggcg
     1561 gcggacatat agtcaccggc aatcgccagc ccgttctgga agccagtgat attgccgcct
     1621 gcggtgtagt agtcgctacg agaacgtacg cgttttgatg cccagtaggt aatgccgagc
     1681 gtaaacacga cgaaaatcag gaacataata atcgcctgcc agttcgttgg ctggcgctct
     1741 acggccccgc taatagcatc cgcggcgtta gctgcgaaag ggagtgtggc ggcaagcgcc
     1801 gtcagaactc ttttcatgat gcttgtacct catgcaggac ttcgttatta agacggtcga
     1861 attcgccgtt cgcccgccag atgtagatac cggtgagaac aaaggagatc acaatcactc
     1921 caacaccaat cggaataccg cgtgtgacgc tggtgttcgg attcagcggc gtgcccagcc
     1981 agccgggcgc gaaggcgatc agtaaaataa agccgatata aactgccagc ataataatcg
     2041 acaggatggt ggcaaaccgt tgccgttttt cgactaactc cctgaaatgc gcattgtctt
     2101 ctatccgctg ataaatagtg ccattcatca cagattctcc agaggtaatg tagggattgt
     2161 tttaattccc gctcccttat gggagaaggt taacgctcgg gtaacccttg ccgaatgtag
     2221 gccggataag gcgtttacgc cgcatccggc aatcaatgcc tgatgcgacg ctgtcgcgtc
     2281 ttatcaggcc tacaaaccgt taccgactcg catccggcaa ttgtgggtta cgatggcatc
     2341 gcgatagcct gcttctcttc aagcagcttc tcgactacgc caggatcggc aagcgtcgag
     2401 gtatcgccca ggttgctggt atcgcccgcc gcaattttgc gcagaatacg gcgcataatt
     2461 ttgccggagc gggttttagg cagggagtcg gtccagtgca gcacgtctgg cgtcgccagc
     2521 gggccaatct ctttacgcac ccagttgcgg acttctgcgt acagttctgg tgacggttcc
     2581 tccccgtgat taagcgtgac gtaggcgtag atcgcctgac ctttaatatt gtgcggaata
     2641 cctactacgg cggcttcggc aatcttcgga tgcgccacca gcgccgactc aatctctgcc
     2701 gtccccagac ggtgaccgga gacgttcagc acgtcgtcca cacgcccggt gatccagtaa
     2761 tagccatctt catcgcgacg cgcgccgtcg ccgctgaaat acatattttt gaaggtggag
     2821 aagtaggtct gttcaaaacg ttcgtgatcg ccaaacagcg tacgcgcctg acccggccag
     2881 gagtcggtga tcaccaggct gccttcggta gcgccttcca gcgggttacc ttcgttatcg
     2941 accagcgccg gttgcacgcc gaagaacgga cgtgttgccg aaccggcttt cagctcggta
     3001 gcgccaggca gcggcgtgat catgaaaccg ccagtttcgg tctgccacca ggtatcgacc
     3061 accggacatt tctcgttgcc gatttttttc cagtaccact cccacgcttc cgggttaatt
     3121 ggctcgccca cggaaccgag aatgcgcagc gacgaacggt cggtgccttc gatcgcttta
     3181 tcgccttccg ccatcagcgc gcggatcgcc gtgggtgcgg tatagagaat attgacctga
     3241 tgcttgtcca ccacctgcgc catacgggca ggcgtcggcc agttgggtac gccttcaaac
     3301 atcagcgtgg tcgcaccgca ggccagcggg ccgtacagca agtaactgtg tccggtcacc
     3361 cagcccacat cggcggtgca ccagtagata tcacccggat gataatcaaa gacatattta
     3421 aaggtcagcg ccgcgtacac cagataaccg ccggtagtat gcagcacacc ttttggctta
     3481 ccggtagaac cggaggtgta gagaataaac agcggatctt cggcgttcat ctcttccgcc
     3541 tggtgctgat cgctcgcttg ctcaaccagg tcgtgccacc acaggtcgcg cccttcctgc
     3601 cagtcaattt tcccgccagt acgcttcagt accaccacat gctctacgct ggtgacgttc
     3661 gggtttttca gcgcgtcatc aacgtttttc ttcagcggaa tactgcgccc ggcacgcaca
     3721 ccttcgtcgg aagtgatcac cagtcgtgag ttggaatcaa taatgcgccc ggcaacggct
     3781 tccggcgaga agccgccgaa aatcaccgaa tgcaccgcgc caatgcgggc gcaggccagc
     3841 atcgcaaccg cggcttccgg caccatcggc atataaatcg ccaccacatc acctttttta
     3901 atgcccagct cgagcagggt attggcgaag cggcagacgt cgcggtgcag ctctttatag
     3961 ctgatatgtt tgctctggct ggcgtcgtcg ccttcccaga tgatggcggt gcgatcgccg
     4021 ttttcttgca gatggcggtc aaggcagttt gccgccagat tcagcgtgcc gtcctcgtac
     4081 catttaatgg acacattacc gggggcaaag gaggtatttt tcaccttctg gtaaggtttg
     4141 atccagtcga gaatttttcc ctgttcgccc cagaaggtat caggtacgtt aatagattgt
     4201 tgatacatcg cctcgtactg ctgagggttt atcaggcaac ggtctgcgat gttggcagga
     4261 atggtgtgtt tgtgaatttg gctcatgctt ttgttctcct tgtaggatgt taataatatg
     4321 tggcataagc gttaaatgta ggggtattgg cagttttgtt tagtatttgg gcgacagatc
     4381 acgcaaaagt agaattgtgc aaataaacgg cagggtaatt tttgaaggtc agcaacaaaa
     4441 gttgattaat tctttgagga acatgcagtt atgcatgctg ttgaaaaaga ggaagatact
     4501 gactaactct aaagtggtat tttacatgca cttacaattg attaaagaca acattcacag
     4561 tgtggttatt tgttacacat aggggcgagc aatgtcatga cagtgtaggt gcggttactg
     4621 tcgtgaaaac aataaaaacc gccattgcaa caatggcgca attcggatga agcccctatg
     4681 acaaggataa aaataaacgc acgccgtatc ttcagcttat tgattccttt tttctttttc
     4741 acttctgttc acgctgaaca aacggcagct cccgcaaaac ctgtaactgt ggaagcgaag
     4801 aatgaaacct ttgccccgca gcatcccgat caatatctct cctggaaagc cacctcggaa
     4861 cagtcagagc gtgttgacgc cctggcggaa gatccacggc tggtgatcct gtgggcgggg
     4921 tatcccttct cgcgcgatta caacaagccg cgtggacatg cctttgctgt gaccgatgtg
     4981 cgtgaaaccc tgcgtaccgg tgcgccgaaa aacgctgaag atggtccgct accgatggcg
     5041 tgctggagtt gtaaaagccc ggatgtggcg cgtctgatcc agaaagacgg cgaagatggc
     5101 tacttccacg gtaagtgggc gcgcggcggc ccggaaatcg tcaacaactt aggttgtgcc
     5161 gactgccata acaccgcctc accagagttc gccaaaggca aaccggagtt aaccctttcc
     5221 cgtccgtatg cggctcgcgc gatggaagcc attggtaaac cttttgagaa agccggacgt
     5281 ttcgaccagc aatcgatggt ttgcggtcag tgccatgtgg agtattactt cgacggcaaa
     5341 aacaaagcgg ttaaattccc gtgggatgac ggcatgaaag tcgaaaatat ggagcagtat
     5401 tacgacaaaa ttgccttctc tgactggact aactccctgt cgaaaacgcc aatgctgaaa
     5461 gcgcagcacc cggaatatga aacctggaca gcgggcattc acggtaaaaa caacgtgacc
     5521 tgtatcgact gccatatgcc aaaagtgcag aacgccgaag gcaaactcta caccgaccat
     5581 aaaattggta atccgtttga taacttcgcc cagacttgtg cgaactgcca tacccaggac
     5641 aaagctgcct tgcaaaaagt ggtcgcggaa cgtaagcagt cgattaacga cctgaaaatc
     5701 aaggttgaag atcaactggt tcacgctcac ttcgaagcga aagcagcgct ggatgcaggc
     5761 gcgacggaag ccgaaatgaa gccaattcag gacgatatcc gtcatgccca gtggcgctgg
     5821 gatctggcga tcgcttccca cggcattcat atgcacgcac cggaagaagg tttacggatg
     5881 ctcggtacgg cgatggataa agcggcggat gcacgcacca aactggcgcg cctgctggcg
     5941 accaaaggca tcacccatga aatccagatc ccggatatct caaccaaaga gaaagcccag
     6001 caggctattg gcctgaacat ggaacaaatc aaggccgaga agcaggactt catcaaaacg
     6061 gtgatcccgc agtgggaaga acaggcacgt aaaaacggtc tgttaagcca ataaccccgt
     6121 tccgcctcgc aaggggcgga aaacacaatg gagtgaatat gagcgtatta cgttcgttat
     6181 taactgccgg ggtgctggcg tcgggcctgt tgtggagcct gaacggaatt accgctaccc
     6241 ctgcggcgca ggcatctgat gatcgctacg aagttaccca gcagcgtaac ccggatgccg
     6301 cctgtctgga ctgtcataag ccagataccg aaggtatgca tggaaaacat gcttccgtta
     6361 tcaacccgaa taacaaactg ccggtcacct gcaccaactg tcacggccag ccatcaccgc
     6421 aacaccgcga aggggtgaaa gatgtgatgc gctttaacga gccgatgtac aaggttggag
     6481 agcagaacag cgtctgtatg tcctgtcatc tgccggaaca gttgcaaaaa gcgttctggc
     6541 cgcacgatgt ccacgtaacc aaagtggcgt gcgccagctg ccattccctg catccacagc
     6601 aagatacgat gcagacgtta agtgacaaag gacggattaa gatttgcgtc gattgccaca
     6661 gcgatcagcg caccaatccg aactttaacc cggcgtctgt tccgttgctt aaggagcagc
     6721 catgacctgg tctcgtcgcc agtttctcac cggcgtcggc gtgctggcag ccgtcagcgg
     6781 caccgcaggg cgcgtggtgg cgaaaacgtt gaatatcaat ggggtgcgtt acggcatggt
     6841 gcatgacgag tcgttatgca tcggctgtac cgcctgtatg gatgcttgtc gggaagtgaa
     6901 caaggtgccg gaaggcgtct cgcgcctgac gattattcgt agcgagccgc agggcgaatt
     6961 tcctgatgtg aaatatcgct tcttccgtaa gtcttgccag cactgcgatc atgcgccgtg
     7021 cgttgacgtc tgcccgaccg gtgcgtcttt tcgtgatgcc gctagcggga ttgtcgatgt
     7081 taatcctgat ctctgcgtcg gttgtcagta ctgcatcgcc gcctgtccgt accgcgtgcg
     7141 ctttatccat ccggtcacga agacggcgga caaatgcgat ttctgccgta aaaccaattt
     7201 gcaggccggt aagctgcccg cgtgcgttga ggcttgcccg acgaaggcgc tgacgtttgg
     7261 caatctggac gatcctaaca gtgagatttc gcaactgctg cgccagaagc ctacttaccg
     7321 ctacaagctg gcgctgggaa ccaaaccgaa gctgtaccgc gtaccgttta aatacgggga
     7381 ggtgagccaa tgacgcagac ttccgcattt cattttgaat cgctggtgtg ggactggccg
     7441 attgccatct acctgttttt gattggtatt tctgccggtc tggtgacgct ggccgtgctg
     7501 ttacgtcgct tctacccgca ggcgggcggt gcagacagta cgttgctgcg caccacgctg
     7561 attgtcgggc cgggcgcggt gatcctcggt ctgttgatcc tcgtcttcca cctgacaaga
     7621 ccgtggacct tctggaagct gatgttccac tacagtttta cctcggtgat gtcgatgggg
     7681 gtgatgctgt ttcagctcta catggtggtg ctggtgctgt ggctggcgaa aatctttgaa
     7741 catgatttgc ttgccctgca acaacgctgg ttgccgaagc tggggatcgt gcaaaaggtt
     7801 ctgagcctgc tgacgcccgt tcatcgcgga ctggaaacat tgatgctggt gttggcggtg
     7861 ttgttggggg cttataccgg ctttctgctg tcggcgctga aatcgtatcc gttcctcaat
     7921 aacccgatcc tgccggtgct gttcctcttc tccggcatct cgtccggtgc ggcggtggcg
     7981 ctgatcgcca tggcgatacg ccaacgcagt aacccgcatt ccacggaagc gcagtttgta
     8041 caccgtatgg aaatccccgt ggtatggggt gaaatcttcc tgctggtggc gttttttgtc
     8101 ggtctggcgc tgggcgatga cggtaaagtg cgtgcgctgg tggcggcatt aggtggcggt
     8161 ttctggacgt ggtggttctg gcttggtgtc gccgggctgg ggctgattgt gccaatgttg
     8221 ctcaaaccgt gggtcaatcg cagttccggc attcctgccg tgctggcggc gtgtggggcc
     8281 agtctggtcg gcgtgttgat gctgcgcttt ttcattctct acgccgggca gttaacggtg
     8341 gcgtaagcca gaaaagaggt ggtttctgga cgtattcctt cctgaagtcg gttttctggc
     8401 gttgttgtta agtctcgggg tcaacgtgtt gaccccgttg acggcctttg cgggagtgcg
     8461 gttgcgctgg cctgccatga tgcgactcac ttgcatcggc attctggcgc agttcgcgct
     8521 cctgttgctc gcctttggcg tactgacgta ttgttttctc atcagcgatt tctcggtcat
     8581 ttatgtcgcc caacatagct acagcctgct gtcgtgggaa ctcaagctgg cagcggtgtg
     8641 gggcggccat gaaggttcgc tgctgctttg ggtgctgctg ctttccgcct ggagcgcgct
     8701 gtttgcctgg cattatcggc agcaaaccga tccgctattt ccgctgacgc tagccgtttt
     8761 atctctcatg ctcgccgcac tgctactgtt tgtagtgctg tggtccgatc ccttcgtgcg
     8821 gatatttcca ccagcaatcg aaggccgcga tctcaatccg atgctgcaac atcccggtct
     8881 tatctttcat ccaccgctgc tttaccttgg ctatggcggt ttgatggtgg cggcgagcgt
     8941 ggcgctggcg agtttactgc gcggcgagtt tgatggtgcc tgcgccagaa tttgttggcg
     9001 ctgggcacta cctggctgga gcgcattaac ggcggggatc atcctcggtt cctggtgggc
     9061 ctattgcgaa ctgggctggg gcggctggtg gttctgggat ccggtggaaa acgcctcttt
     9121 attaccctgg ctttctgcca ctgcgctgct gcacagtttg tccctgacac gccagcggga
     9181 gattttccgc cactggtcgc tgttgctggc gatagtaact ctgatgctgt cgctactggg
     9241 taccttaatt gtccgttctg gcattctggt ttcggttcat gcgttcgcac tggataacgt
     9301 ccgcgccgtg ccgttgttca gcctgtttgc actgattagc cttgcgtctc tggctctgta
     9361 tggctggcga gcgcgggacg gtggcccggt ggtgcgtttt tcggggttat cgcgggaaat
     9421 gttaatcctc gctacgctgt tgctgttttg cgcagtgcta ctgatcgtgc tggtgggaac
     9481 gctttatccg atgatttacg gtctgctggg ctggggacgc ctctccgttg gcgcgccgta
     9541 ttttaaccgc gcgacgttac cgtttggcct gttgatgctg gtggtgattg tgctggcgac
     9601 gtttgtctct ggcaaacgcg tgcagcttcc ggcgctggta gctcatgcgg gcgtgctgtt
     9661 atttgccgct gggatcgtgg tttccagcgt cagccgtcag gagatcagcc tgaatttaca
     9721 gccgggtcag caggtgacgc tggcaggata caccttccgt tttgagcgcc tcgatctgca
     9781 agccaaaggc aattacacca gcgaaaaagc gatagtggca ctgtttgacc atcagcaacg
     9841 cattggtgaa ctgatgccgg agcggcgttt ttacgaagca cgtcgtcagc aaatgatgga
     9901 accgtcaatt cgctggaacg gcatccatga ctggtatgcg gtcatgggtg aaaaaaccgg
     9961 agcggatcgt tacgcttttc gcttgtatgt acaaagcggt gtgcgctgga tctggggggg
    10021 aggattgttg atgattgcgg gcgcattgtt aagcggatgg cgggggagga agcgcgatga
    10081 ataaaggact tctcacgcta ctgctgttat ttacctgttt tgcgcgcgct caggtcgtag
    10141 acacctggca attcgccaat ccgcaacaac agcaacaggc gttaaatatt gccagccagt
    10201 tacgttgtcc gcagtgccag aatcaaaact tactggaatc caacgcgccg gtggcagtca
    10261 gtatgcgcca tcaggtttac agcatggtgg cggaggggaa gaacgaagtc gaaatcattg
    10321 gctggatgac cgaacgctac ggagattttg ttcgctataa cccaccgtta acgggtcaga
    10381 cgctggtgtt gtgggcgctg ccagtggtgt tgttactgct gatggcactg atcctctggc
    10441 gagtgagggc gaagcgatga aagagcccaa aataccggtg aaaatgctga caacactcac
    10501 gattttgatg gtatttctct gtgtcggcag ttatctgtta agtccaaaat ggcaggcggt
    10561 acatgcggaa tatcagcgtc agcgcgatcc gctacatcag tttgccagcc agcaaacccc
    10621 ggaagcgcag cttcaggcat tgcaggataa aatccgcgct aatccacaaa acagcgaaca
    10681 atgggcgtta ctgggcgagt actatctgtg gcaaaacgat tacagcaatt cgctgctggc
    10741 gtaccgtcag gcgttgcaac tacgtggtga gaacgctgaa ctgtatgcgg cgctggcgac
    10801 ggtgctttat taccaggcca gccagcatat gaccgccctg actcgcgcaa tgatcgacaa
    10861 agccctcgcg ctggactcta acgaaatcac cgctctgatg ctgctggctt ccgatgcgtt
    10921 tatgcaggcg aattacgcgc aagccatcga actatggcaa aaagtgatgg atctcaactc
    10981 accccggatc aaccggacac agctggtcga gtcgattaat atggcgaaat tgttgcagcg
    11041 gagatcagat taatcatctg gtgatgaaga ttatgttatt gagttgtata aaaaataatc
    11101 gaatgaacat atgccaaaaa taatcaccaa tcagtattat tgcagattaa acaaataaaa
    11161 atctttccat aacaaatggt tattcattaa tcctgccata aactcgcgtt attatgcatt
    11221 aatgcagcga aaagctctgt tgttaaaggg ttgcgcaaca taccgcgcaa atgatactga
    11281 tcataagcgt taaaaaaatc tacaaaccaa cgcaacacaa ttcatgccct ggcagtatgt
    11341 cacgttctcg cgtttctgaa cggggaacgg cgctccattg aggaagttat tctgatgaaa
    11401 agtataaaat tcagcctggc ctggcagatt ctgtttgcta tggtgctggg cattctcctg
    11461 ggaagctacc tgcactacca tagcgacagc cgcgactggc tggtcgtcaa tttgctctct
    11521 ccggcgggtg atatcttcat ccatctgatc aaaatgattg ttgtgccgat tgtgatctcc
    11581 acgctggtgg tgggtatcgc gggtgttggt gatgccaaac agctcgggcg tattggcgcg
    11641 aaaaccatta tttacttcga ggtgatcacc accgtcgcca tcattttggg gatcactctg
    11701 gcgaacgtct tccagcccgg tgccggggtg gatatgtcgc agctggcgac cgtcgatatc
    11761 tcgaaatatc agagcactac ggaagcggta caaagcagtt cccacggcat tatgggcacg
    11821 attttgtcgc tggtgccgac gaacattgtg gcgtcgatgg cgaaaggcga aatgctgccg
    11881 atcatcttct tctcggtgct gtttggtctg gggctttcct ccctgcccgc gacgcatcgt
    11941 gaaccgctgg tgaccgtgtt ccgctccatc tctgaaacca tgtttaaagt gactcacatg
    12001 gtgatgcgtt atgcaccggt gggtgtgttt gcgctgattg cggtgacggt ggctaacttt
    12061 ggtttctctt ctctgtggcc actggcgaaa ctggtgctgc tggtgcattt cgccattctg
    12121 ttcttcgcgc tggtagtgct gggaattgtg gcgcgcctgt gcgggttaag cgtctggatc
    12181 ctgattcgta ttctgaaaga tgagctgatt ctggcgtact ccactgccag ctctgaaagc
    12241 gtactgccgc gaattattga gaagatggaa gcctacggag caccggcgtc gatcaccagc
    12301 ttcgtggtgc cgaccggtta ctcttttaac cttgatggtt cgacgctgta tcaaagtatt
    12361 gccgctattt tcatcgctca gctgtatggc attgacctgt ccatctggca ggaaatcatt
    12421 ctggtgctga cgctgatggt gacctcgaaa gggattgctg gcgtgccggg cgtgtcgttt
    12481 gtggtgctgc tggcaacgct gggtagcgta ggtattccgc tggaaggcct ggcgtttatt
    12541 gctggtgttg accgtatcct cgacatggcg cgtactgcgc tgaacgtggt gggtaatgcg
    12601 ctggcggtgc tggtgattgc caagtgggaa cacaaatttg accgcaaaaa ggcgctggct
    12661 tatgagcgtg aagtgctggg caaatttgat aaaactgcgg atcaataatt gaagattgcc
    12721 ggggttatcc acccggcaat gtgtggatgc ctgatgcgac gctggtgcgt cttatcaggc
    12781 ctaccttaac cgttggttaa tttttcaaac tcttcacacc cggtatcaaa cccttccata
    12841 cagctcaggt tcaaccagtg cagcgccttt tgtttgttct tctcgataaa accttcctca
    12901 ccgtttaaga acatcattcc cgcccagtac tcggaataac cggttcggga aattgcagag
    12961 ctgcgtttga aataccaggt tgccttgtca tcgtcggcct taatgcccac gccgttggcg
    13021 taaatcaaac caagcagcat ttgggcatcg acggcggagt cattctccag atcttccgag
    13081 gcgttttcta acagcgaaat cgcctttgga taatccggtt tacccgcttg agtatttacc
    13141 agaatatgcg ccagcgttac ttcaccttct ttactgcccg cttgcgccgc tttctctgca
    13201 agaacttttg cttgtggata gtccagactg accggattgg tgattttgat ctgcgccagc
    13261 agcgcgcagg catcagcatc accgctgtcg gcggctttct gtgcccaata ttcggctttg
    13321 ctcaaatcgc cggagctaaa ccagctgtcg gcaagaaaat attgtgcgcg tcggtccccg
    13381 gcctctgctg cctttaaata ctggctgcca ggctcggagt cgttcgcgtg ggcaaagaat
    13441 gtcaaaaaca acatcaatgc gataattttt ttcattttga taatacgatt gggtagggag
    13501 taaccagtat aaagagttcg aatggataaa aaaacagcct ccgaaaggag gctgtagaaa
    13561 ggactgtatt acgccagtgc cgcttcgcgc aggcgagttt tcaacttgtt gtactcgtca
    13621 atcacgtact gctcggcggc gcgctgatcg gcgatcggct cgacgcgcac ggcgcagtat
    13681 ttgtattccg gcgttttcgt aatcgggctt aagttttcgg taaccagctc gttacaggca
    13741 ccaatccacc actggtaggt catgtaaatc gcccctttgt tcggacgatc gctgacctgc
    13801 gcacgggtga taattttgcc tttacgcgag tgcacccaaa ccaatgcctc atcttcaata
    13861 cccagacgtt tggcgtcttc ggtattgatt tgtgcgtagc caggttcatc agccagcgcc
    13921 gccagtgccg cacagttacc ggtcatcgaa cggcaagagt agtgaccaac ttcacgcacc
    13981 gttgacagta ccatcgggta ctcgtcggtg agtttgtcga ttggcgctac ccagtcgcag
    14041 gtgaagaact gtgccagacc gttcggggta tcaaacttct ctttaaacag ataagaagtc
    14101 ccctgatcgg catctgaagt atcgcggcaa ggccactgaa tgaagcccag ttcgcccatt
    14161 ttctcgtaag tcgcaccgta gaaatccggg cacagatgac gcaactcatc ccagatctcc
    14221 tgggtgttgt tgtagtgcat cggataaccc atacgggtgg cgatttcact gatgatttgc
    14281 cagtccgttt tcagatccca tttcggttca actgctttga agaaacgctg gaagccacgg
    14341 tcagccgcag taaacacgcc ttcatgctcg ccccacgacg ttgacggcaa aatgacatcc
    14401 gccgccgacg cggttttggt cataaagatg tcctgaacga taaccagttc cagatcttca
    14461 aaggctttac gtactgccga cagctccgcg tcagtttgca gcggatcttc gcccataatg
    14521 tacgcggcac gcacttcgcc atgcgctgcg cggtgcggca gctcgctgat gcgataaccg
    14581 gtatgagcag gcaggctttc cacgccccag gctttggcga atttctcgcg gttagccgga
    14641 tctttcacgt actggtatcc cggatacgta tccggcagcg cgcccatatc gcaggcaccc
    14701 tgcacgttgt tctgaccacg aaccgggttt acacccgcat gcggcttacc gaggttaccg
    14761 gtcagcatcg cgaggctggt cagagaacgc acggtttcca cgccctggta gaactgggtt
    14821 acacccatgc cccacaggat ggcggcgctt ttcgcctggg catacatccg tgccgcctga
    14881 cgaatctcac tggcgctgac gccggtgata tcttcaaccg actccggcgt gtagccttca
    14941 acgattttac gatactcttc aaagccttct gtacgggaag cgacgaacgc tttgtcgtac
    15001 agattttctt caataatgac atggcccatc gcattcaaca gcgcgatgtt cgagccgttt
    15061 ttcagtgcaa tgtgcatgtc agcaatgcgc gcggtttcaa ttttgcgcgg atcgcagacg
    15121 ataattttcg ccccgttacg tttagcgtta attacgtgat tcgccacgat tgggtgggaa
    15181 tccgccgggt tgtacccgaa aacgaacact aaatcggtat tatcaatttc gttaatagca
    15241 ttgctcattg cgccattacc gaccgattgg tgcagacctg caaccgatgg gccgtgtcag
    15301 acacgagcgc agcagtcaac gttattggta ccaataacgg cgcgcgcaaa tttttgcatt
    15361 acatagttgg tttcgttacc cgtaccacgc gaggagccgg tcgtctggat ggcatccgga
    15421 ccgtacttct ctttgatggc gctcaggcgc tcggcaacgt aattcagtgc ctcatcccag
    15481 gaaacaggtt cgagtttgcc gccacgctgg cgacggatca tgggggtttt caggcgcggg
    15541 gtcaggatct gggtatcgtt aatgaaatcc cagccataat aacccttcag acacagggta
    15601 ccctggttgg ttttcccctg cgctgcctcc gcccggacga ttttgccatt atcgacgacc
    15661 aggttgattt tgcaacctga tgcgcaatag gggcaaaccg tgacgacttt tttcatcggt
    15721 ctcgctccag ttaatcaaat cacgcatacg cgctctcgac tacagtatgc atcttttatg
    15781 ccacatttta tttggggtca ttccctgata ttacgggcac tatttattca aaactctgac
    15841 gaaaaacagg ctgtcgtcag ttttgacgtg acgaaacgaa ataccgcgtg acagccatca
    15901 cgcggcagac attttatttt ttctcgacga cgggagccgc ctgatacccg ccgcccagcg
    15961 atttcatcaa ctgaatgctt tggatcaccc ggcggctgtc cagcatcagt aatgacatct
    16021 cttcggcaag tactggcaac cgggcttcag tggcctgtaa gcggctggtt aagccgcgct
    16081 gataggcggc ctcggcagcg cgctgggtga agcgcgttgc ttccacgcgt tcagcctgca
    16141 tttctcgctc gtcgttgagc gtttgcagac gcgtgccgtt gacggcaacg tcacgcaccg
    16201 cgttcagtac tgactggttg taacgttcaa tcatcatgtt gctggcggcg cgcgtgcctt
    16261 cgagattggc attcaaccgt ccaccgtcaa acagcggcaa tttcagaccc gggatgaagt
    16321 tgaactggcg actggttttt ttgaataagg tatccaggtg gatggcatca aggccgaaaa
    16381 acgctttgat atcaaagctc gggtagaaca gcgcccgcgc ggaatccacc tgatctaatg
    16441 acgcctgaac ataccagcgc atggcttgca gatccgggcg tctggcgagc aactcataag
    16501 agagtgtcgc cggaatgccg gtctggactc gtggtaatgc caccggtttg atctccggca
    16561 tatcgctggc ccccgcgcca atcaatgcac gcagagattc tcgcgtttcg gtgatttgcc
    16621 ctttgacggc agcaatttgt ttatcgaccg ccagaatctg tgcccgcgcg ccgtggaaag
    16681 gcacttgtgc ttccagaccg tgcgccactt tactctggtg cgctttcacc gcgtaatcaa
    16741 tcacatcgcg agtttgttct aacagatcga gcatctgata gctggcctgc atactgtaat
    16801 aaagctgcgc tacgcccgtg gtcagcgata gctctactgc tgcggtttct gccagcgcgg
    16861 cattatgcgc gccaatggcg gcggcaaccg ctgagcgatg cacaccccac aaatcaagat
    16921 ccagtccggc aaacaaacct actgtggctt ccgtatagta cggcccgtcc atacccagtg
    16981 cgggcgcatc catcgcataa gggcttaaaa agccgttcgc cgagacgcgt tggcggttga
    17041 gcatccctaa cgctgcgacc tgtaattgtg aaccggcatc taacaaatcg gcctgggact
    17101 gcgctttttc ttcccgcagt ttcgcttcgg cgagggtgtg tgaaccactt agcgtccgtt
    17161 ggatcagcgc atccagctgc gggtcattga gttgtttcca ccactgcgcc tgcggccagc
    17221 cggagctggc aagatgaata tcgtcggcca gtttgatttg ttccggtttg agctgttgat
    17281 gaggtgcaga atccttacgt accagggcac agccggaaat cagcgtcgtg ctgccgagaa
    17341 tgctgcacaa cagcagacgt gaaagttgac gattgatcat tgcgtggctc cttgtgcctg
    17401 ttccgtggcg ggcgttaatg ccggggctgt ccagtctggc aagcgggcga aaagctggca
    17461 gacgtgttgc tcctgtttta ataaggtggg caggatggcc tgcgaggcgg gtgtctcttc
    17521 aagcgttatt tgaggagaac ggctgagtgc ggttgccaga ccggcagcgt atttctccag
    17581 ggcgtcggca aactgagctg ctctgtccgg ctgtagtgcg ttatccagcg cctgcgccga
    17641 gttccaggtg gcatcccagg cgtgaagaat atcgcggccc tgacgaataa ccgtttgcga
    17701 acgttcaatc agtaaggcgc gttcttcgct gtccagttga cgctccagcg ccacgcgttg
    17761 gcacatctct tcacaggcat taaacgccgc atgcagaccg atacgaattt gcagataagt
    17821 gcgcagagcc gtgacttcct gctggcgtgg gatccgcatt actttactta acatacccag
    17881 cgtgccagcc agtttttgcg gcagtgtgcg cgcttcactt tcaggccaga caaaggtgta
    17941 aatcaccgcg gacaccacgg taccaatgat gatacccatg gcgcgatcgc gaatttccac
    18001 caggtcgtac accgggccaa aaacgttttc gagcgtggcg agcgcgaagg tgaccaccat
    18061 ctgtgtgccg atataagaag agcgttcaga gctggtggca atccatgcgc ccaacaggaa
    18121 aatcggtgcc agcacaaaca gcaattcgac aatattgtcc agccagggca tgaccagtag
    18181 cgtgaataac agcgccagaa tcgcgccgca aaaggccccg ccaaaacgca gcaccatctt
    18241 ctggtacgac gaaccgacat ttgggttagc gacgatcacg catgtcagca tacaggtgtg
    18301 aatgccttcc cagtccacgc cgctgtagaa ggtgtaacag atcaaacagg cgagcagtgt
    18361 ttttaccgcg tagcgcatat agtctggatt ggtaaaagca tcggcgacca ttgatggcgg
    18421 tttggcggcg ggcgttggcg gcgtattcgg gtccatctga cccagttgta acaacgtctg
    18481 gcagatattc tccaggttac attcccgtgc cgccatcgct tcactttcag tgatccgcca
    18541 gtcgctttgc cagcactgac cttcagcaac ggcatgctgc agcttattga tttctgaggc
    18601 taatttttgc cgaaattcaa taattgcctg agaatcagca aaagaggtgg gatcgtagcg
    18661 attcagcgtc gagtaaatgt aggttaccgt tgccacgcag ctttgccacc atgcgctttg
    18721 agttcgccag ttggcatcgt ccgcgagaca aaagacattc agtttttgca gcgccagcgc
    18781 ctctctttca atccgcgttt cgggtagcgg tgcgaggctg tccgtcaggt gactaatggc
    18841 atcatcaagc cgatcattaa gcgcttgatg catttgcgta atggcacgac tgggaaacca
    18901 cagcacgccg attaacgtca tcagcaaggt tggatagagg ccaacaacga tacaccacag
    18961 cgttaagcgc acgaccactt ccggatagtc gagcatggcg gggaaggttt gcccgtaaat
    19021 ggcgacaatg gcgacagcga aaaagaccag tcccagccga tgggtgcgca tcaaaaacat
    19081 acagcccatc aggatcggcc cggcgatgat caatcggatc aacggttcgc cgtatgacca
    19141 tttgtagatc aaaaacaggc tgccgatctc cagcaccgtg gcaaccacaa acaagatcgc
    19201 gacaaatttg gtgtaaaacg cgttcgactg aataccgtaa aacagcactg ccagcgataa
    19261 cgccacaaaa gggatctcaa aagtcatcga gatcagaatc accagcaggc agcctaccca
    19321 gagttgcacg atctgcggca cgcggcctgg tcgccgctcg cttaactctt catgaaagaa
    19381 cgccagcagc ctgaccaccg gtaatggcag ggagttgagc gcgctcatta ttgcggctca
    19441 agattagcga ctgccgaagc gccgatgcgg aacatttccg ggtcaggttt atcgaccatg
    19501 attttgaccg gaaaacgctg ggcaacgcgg acccagttaa tagagcgaga aactttcggc
    19561 aggccgccca gcaccaggcc gccgtcatcc ggtagcacgc cgtagccaat cgaatccact
    19621 ttaccctcga aggttttgcc gctgtcactc atcaggcgaa tcgttgcggg tgtacctgag
    19681 cgaatatttt tcagatcggt ttcgcggaag ttggcgatca cgtaccagtg acgagtgtcg
    19741 attagggtaa aaatagggcg catggcagaa gcaaattgcc cgatggaggt tttgagggaa
    19801 atgacccggc catcaaacgg cgcgcgaacg gtcgccattt ccagatgaag tttggtcagg
    19861 gcaatatccg cttcgaccgc cgcacgttgc gccaccagcg catcgacgcc gctgacggcg
    19921 ctggcggctg actgcgcctg taacaatacg gcattaagat cggcttctgc ggcgcgctgc
    19981 gcagttcttg cacggtcaac gtcttccgct gagacaaaac cttctttcag taatggctcg
    20041 gtgcggcgta atgtatctgt ggcctgtttc gcggcggcac gggctttttc taccgtggcg
    20101 ttaaccgagt cggcaccaaa ctgttgcgcg tcaacgctac gctgggtgag cataatttgc
    20161 ttatccagcg ccgcgaggga ggcttcagct ttcgccagat tggcttcgta cgggcgcggg
    20221 tcaatgcgga acagcaaatc gccctgtttg actgcctggt tgtcggtgac cgccagttct
    20281 acaatgcgac cgctgacttc cggcaccaca tcaatggtat ctgctgacgc gtaagcgtca
    20341 ttagttgatg gcgcactgtc tacgcgccag ataacgaaaa caagggcaac cagcgccaac
    20401 gcaaccacta acagagcagg gaatttactg cgaggagctt ttttcggcgt actttccatg
    20461 agcgcgtctc aaatagatta gaaaaatgcc agccagaaca gcatggcgta gagagcaaaa
    20521 agggcggtat aaataattcc gacaaatgcc agattgatat ttgcccgctg aataactcgc
    20581 ctcgtgatga gcatcaaaat aaggctggca atggcgcaga aaaaccagct gggataataa
    20641 gcaccgatca ccgggattgc cggagataat gagcatcccg aaaccatgaa gacgggaatt
    20701 atccaggctg tttttttgag ttgcatcgcc atgcgagaga gaactgttgg catattaaat
    20761 acatcctgtc tgctgcgaac aatatctgta gtgaatatca atatccattg aaggcgtcag
    20821 tatggctatt ttctcgatga ataaaaatag ctt
//
LOCUS       DAPHDW010000072        20572 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.072, whole genome shotgun sequence.
ACCESSION   DAPHDW010000072 DAPHDW010000000
VERSION     DAPHDW010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.072"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..702
                     /locus_tag="RH057_002672"
     CDS             1..702
                     /locus_tag="RH057_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001667874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV3129022.1"
                     /translation="MESQKILFALSTPMEIRNECCLPSHSSPKMYLGTCFFDLSSSWG
                     IDARDDLLRTIHRMIDNGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQAYAQFVAS
                     TAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRYYRNWRQYF
                     AGYTFGRQYWQSPEDDHLQLLREFLARKEYDDSGNDMFYQLFASDDAYYPTLSWQPLA
                     YYSACPETLKDMSDL"
     gene            699..2126
                     /locus_tag="RH057_002673"
     CDS             699..2126
                     /locus_tag="RH057_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HDV3129023.1"
                     /translation="MKNCWKILEIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSAGRLDIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCD
                     YWREYPSTQADALILQWCRQHRVDYYPLVVMMIEARELVNDQGKQLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(2136..2690)
                     /locus_tag="RH057_002674"
     CDS             complement(2136..2690)
                     /locus_tag="RH057_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3129024.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTTLRDKAMVNYAFDYLSSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            2791..3498
                     /locus_tag="RH057_002675"
     CDS             2791..3498
                     /locus_tag="RH057_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HDV3129025.1"
                     /translation="MNKEEQYLLFALSAPMEILNQGCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWHRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRVGVLNNWLTEEESLWLQSRVYVRAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNTSLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            3495..4946
                     /gene="djlC"
                     /locus_tag="RH057_002676"
     CDS             3495..4946
                     /gene="djlC"
                     /locus_tag="RH057_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HDV3129026.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVGEADNEDKDAAAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMAELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLAELCA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRLLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(5006..6676)
                     /gene="hscC"
                     /locus_tag="RH057_002677"
     CDS             complement(5006..6676)
                     /gene="hscC"
                     /locus_tag="RH057_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
                     /protein_id="HDV3129027.1"
                     /translation="MDNAELAIGIDLGTTNSLIAVWKDGAAQLIPNKFGEYLTPSIIS
                     MDENNHILVGKPAVSRRTSHPDKTAALFKRAMGSNTNWRLGSDTFNAPELSSLVLRSL
                     KEDAEEFLQRPIKDVVISVPAYFSDEQRKHTRLAAELAGLNAVRLINEPTAAAMAYGL
                     HTQQNTRSLVFDLGGGTFDVTVLEYATPVIEVHASAGDNFLGGEDFTHMLVDEVLKRA
                     DVARTTLNESELAALYACVEAAKCSNQSPLHIRWQYQDETRECEFYENELEDLWLPLL
                     NRLRVPIEQALRDARLKPSQIDSLVLVGGASQMPLVQRIAVRLFGKLPYQSYDPSTIV
                     ALGAAIQAACRLRSEDIEEVILTDICPYSLGVEVNRQGISGIFSPIIERNTTVPVSRV
                     ETYSTMHPEQDSITVNVYQGENHKVKNNILVESFDVPLKKTGAYQSIDIRFSYDINGL
                     LEVDVLLEDGSVKSRVINHSPVTLSAQQIEESRTRLSALKIYPRDMLINRTFKAKLEE
                     LWARALGDEREEIGRVITDFDAALQSNDMARVDEVRRRASVYLAIETS"
     gene            complement(6760..7695)
                     /gene="rihA"
                     /locus_tag="RH057_002678"
     CDS             complement(6760..7695)
                     /gene="rihA"
                     /locus_tag="RH057_002678"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HDV3129028.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYSQGMTVV
                     DYYYLTGNKPNATVMVDVDRQGFVDLLADRLKFYA"
     gene            complement(7813..8538)
                     /gene="gltL"
                     /locus_tag="RH057_002679"
     CDS             complement(7813..8538)
                     /gene="gltL"
                     /locus_tag="RH057_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HDV3129029.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMDPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            complement(8538..9212)
                     /gene="gltK"
                     /locus_tag="RH057_002680"
     CDS             complement(8538..9212)
                     /gene="gltK"
                     /locus_tag="RH057_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HDV3129030.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVAWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            complement(9212..9952)
                     /gene="gltJ"
                     /locus_tag="RH057_002681"
     CDS             complement(9212..9952)
                     /gene="gltJ"
                     /locus_tag="RH057_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HDV3129031.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWIWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQAYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAVIMLVMTLVERKVRLPGNMGGK"
     gene            complement(10122..11030)
                     /gene="gltI"
                     /locus_tag="RH057_002682"
     CDS             complement(10122..11030)
                     /gene="gltI"
                     /locus_tag="RH057_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HDV3129032.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            11485..13362
                     /locus_tag="RH057_002683"
     CDS             11485..13362
                     /locus_tag="RH057_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HDV3129033.1"
                     /translation="MSASSVKPLNVQLPAITLILFALCVGIFCYLAQWMSYEEVDQSA
                     LIHLGANVASLTLSGESWRLFSSVFLHSSFSHLLMNMFALLVVGAVAERILGKWRLLI
                     IWLFSGVFGGLISACYALRDSDQIVISVGASGAIMGIAGAALATQLASGAGTYHKNQR
                     RVFSLLGMVALTLLYGARQAGIDNACHIGGLIAGGALGWLSARLSGQNRLVTEGGIIV
                     AGSLLLTGAIWLAQQQMDESVLQVRQSLREAFYPQEIEQERRQKKQQLAEERNALRET
                     LSAPVSREQASGDLLAEIADIHDMAISRDGNMLYAAIENTNSIVVFDLGQKKILHTFT
                     APIAKEKSVKHCGGCKDQGVRSLALSPDEKLIYATSFEANALSVINVATGEIIQSITT
                     GAHPDSLILSRDGTKAWVMNRTSNSVSAIDLVTYQHVADIPLEKYDGTGTSNKPGAWV
                     MALSPDEKILLIPGMVRGDIVRINTITHQKESYPASDARGTISAMRFRPENGDVIFAD
                     SQGISRISVGDQQASIMTQWCSRSVYSVEGISPDGQYLALVSYGLQGYVILLNINAGQ
                     IIGVYPASYVNHLRFSADGRKIFVMAKNGLIQLDRTRSLDPQAIIRHPQYGNVACIPE
                     P"
     gene            complement(13440..14978)
                     /gene="lnt"
                     /locus_tag="RH057_002684"
     CDS             complement(13440..14978)
                     /gene="lnt"
                     /locus_tag="RH057_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HDV3129034.1"
                     /translation="MAFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIAAPSLWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPIMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDGDQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSVNGIELTAAICYEIIL
                     GEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFTREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     ARKR"
     gene            complement(15003..15881)
                     /gene="corC"
                     /locus_tag="RH057_002685"
     CDS             complement(15003..15881)
                     /gene="corC"
                     /locus_tag="RH057_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HDV3129035.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            complement(15971..16438)
                     /gene="ybeY"
                     /locus_tag="RH057_002686"
     CDS             complement(15971..16438)
                     /gene="ybeY"
                     /locus_tag="RH057_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HDV3129036.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            complement(16435..17475)
                     /locus_tag="RH057_002687"
     CDS             complement(16435..17475)
                     /locus_tag="RH057_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HDV3129037.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            complement(17628..19052)
                     /gene="miaB"
                     /locus_tag="RH057_002688"
     CDS             complement(17628..19052)
                     /gene="miaB"
                     /locus_tag="RH057_002688"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HDV3129038.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLHLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRV
                     AETPESVIARTRKENDLGVGYYQP"
     gene            19198..20373
                     /gene="ubiF"
                     /locus_tag="RH057_002689"
     CDS             19198..20373
                     /gene="ubiF"
                     /locus_tag="RH057_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HDV3129039.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVMFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPGSLIALHRDNDLQELELKGGEVIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPVRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
BASE COUNT         5028 a         5063 c         5295 g         5186 t
ORIGIN      
        1 atggagtcgc aaaaaatatt gtttgcactt tccactccga tggaaatacg caatgaatgc
       61 tgtttacctt cgcattcatc acccaaaatg tatttaggta catgcttttt tgacctttct
      121 tcctcctggg gaattgatgc ccgtgatgac ctgctgcgca caattcaccg aatgatcgat
      181 aacggtcatg ccgctcggtt ggcagggttt tatcaccgct ggtttcgtta ttcgccatgt
      241 gaatggcgtg actatcttgc tgaactaaat gagcaaggtc aggcttatgc gcaatttgtc
      301 gccagcaccg ccgaatgctg tggtgaggga ggaattaaag cctgggatta tgtccggatg
      361 ggttttttaa gccgaatggg cgtacttaat aactggttga gcgaagaaga aagtctatgg
      421 atccagtcgc gcattcatct acgggctcta cgttattatc gtaactggcg gcaatacttt
      481 gccggttata cctttggtcg gcaatattgg cagtctcccg aagatgatca tctgcagtta
      541 ttacgcgaat tcttagcgcg taaagaatac gacgattccg gcaatgatat gttttatcaa
      601 ttatttgcca gtgatgatgc gtattaccct accttgtcct ggcaaccatt ggcttactat
      661 tctgcatgcc cggaaacgct taaggatatg agcgacctat gaaaaattgc tggaagattc
      721 tcgaaataga ggaaacgact gacgtcgata ttatccgccg cgcttatctg gcgctgttac
      781 cgtcctttca tccagaaacc gatccgcagg gttttaaaca acttcgtcag gcgtatgagg
      841 aggcgctacg gattgcgcag tcgcctgcta aatctgtttg gcaaccagaa gaatatgagg
      901 tagcagaaca tgaaattctg ctcgcctttc gtgcgttact tgcctctgat agcgaacgtt
      961 ttctgccctc cgcctggcag cgattcattc agcaattaaa ttattgctcg atggaagaga
     1021 ttgatgaatt acgctggtcg ctgtgcacaa tcgccatgaa cactgcccat ttatccttcg
     1081 agtgcgtggt gttattagca gaaagattgc ggtggttgca ggaggaaaac gtcggggaaa
     1141 tagacgaaga agaactggaa tcctttttat atgccattgc gaaggggaat gtttttaact
     1201 tccagaccat tctgcatctg cccgttgccg tgcaaaatga caccatcgat ttttaccaaa
     1261 tgttcgctcg gatttggtca tcgcatccag aatggctgac attgtattta gcgcaacatc
     1321 gcgcagtgat catccccgat gatgcaaaac tgcacagaaa tttactccgc tggtatagcg
     1381 caggtcgcct ggatatcccc gaacttctgg attacgcccg gtcgtggcgg gaagctgaac
     1441 ctgataatga agatgcgcgt tattatgaat acgcgcaacg cgtctattgt ggagaaggcg
     1501 aaagcttgct ggcagaactt tgtgactact ggcgcgagta tccctccacc caggcggatg
     1561 ctttaatatt gcaatggtgc cgtcagcatc gggtcgatta ttacccgtta gtggtgatga
     1621 tgattgaagc tcgtgaactg gttaacgatc agggtaaaca gctactttat gtccccggcg
     1681 acagcgcccg tacgcgtttt catttatacg aaatactcag cgatgaaaaa ctctctgcgc
     1741 tggggcgttc actggtcgag atggttttgc acaaaggacg taagccgcgg atctcactca
     1801 cgcgtgatac agaacatccc ttatggccat tatatttagt tgccaaacaa ttagtgcagg
     1861 ccagccaacc gacagaagaa tcattaatgc cgattgtgag ccgccttgat gcagaagatc
     1921 gttgtccact ggaagcatta attattcgtc gattattaat tcaggcggcg aattttaccg
     1981 gtcaggaaac cgtcgagccg gagccgcaac cgcagccaat gcccgttgac gatggcgggc
     2041 tgggctgtct gggcgtcatc aaaattattt tctacatttt tatctttgcc ggtttgatag
     2101 ggaaaatact ccatctcttc gggtgacagt cgacgttaat gtgcgggaag tccggcaagt
     2161 aatctttccg catttttgta atgttgtttg gcggccagtt ttaaccattt ttgagattca
     2221 taatggttaa cattaatacc agtgccattt ctgtagatca aacccaaagt atattgagca
     2281 tgagcatacc cttgttcagc tgcttcctta taccagagaa ttgctttacg ggtatcgggt
     2341 ttgactccca ggcctttcag gtagatccac cccagtttgc tttgtgcgcg cggattttcc
     2401 tgttctgctg attgctcata ccagtatctc gcctgtgtat aatccagtgg tttaccgtcg
     2461 ctaccatgaa gataaaattc gccaaggcga tattgcgatg ttgagtgacc atgaatcgcg
     2521 gaaagctccg tggctgccgt ggttaacggt agactgcctg gtgagcttaa ataatcaaag
     2581 gcataattca ccatggcttt atcacgcaac gttgttttct tgggttggct aatacaggta
     2641 attgctgcaa ctataaatat tgcgaatata tacattaatt ttttattcat atgtgaatgc
     2701 ttcgattttc tttttatttt gaaagcgtca agtttatact catcaataat aaaaagtagt
     2761 cagttaatgc acttaaggga ttattaacaa atgaataagg aagaacaata tcttcttttt
     2821 gcgctttctg cgcccatgga aatacttaat cagggttgca aacccgcgca tgactcgcca
     2881 aaaatgtata ccgggataaa agaattcgat ctctcatcat cctggggaat aaataatcgt
     2941 gatgatttaa ttcagactat ttatcagatg actgatgatg gtcatgccaa tgatttagct
     3001 ggcttatatc tgacatggca tcgctcttcc cctgaagagt ggaaagcatt aattgctggt
     3061 ggctcggaga gagggttgat ttatacccaa ttcgttgccc aaaccgctat gtgctgtgga
     3121 gaagggggaa tcaaagcctg ggattatgtt cgaatggggt ttctgtcacg ggtcggtgtg
     3181 ctcaacaact ggctgacgga agaagagagt ttatggctgc aatcgcgcgt ctatgtacga
     3241 gctcaccatt actatcacag ctggatgcac tatttttccg cctattcgtt aggacgtctc
     3301 tactggcaat cttctcagtg cgaagacaac acttcgctac gcgaggcgct aaccctgtac
     3361 aaatacgata gcgccgggag tcggatgttc gaagagctgg cagcgggaag cgatcgtttc
     3421 tatgccacgc ttccctggca gccattaact gtccagcctg aatgcccggt aacgcttaag
     3481 gatgtgagcg acctatgaaa acatgttggc aaatcctcga aattgaaagc acgacgcaaa
     3541 tagacattat ccgccaggct tatcttgctc gcttaccgtt atgtcatccc gaaaccgatc
     3601 cgcaggggtt taaagcatta cgccaggcct atgaagaggc gctgcgactg gcggtaaatc
     3661 ctgtcgggga agcagataat gaagataaag atgccgccgc tgaacatgaa atactacgtg
     3721 cattcaggac attactggat tcagaaagtg atcgttttca gccctccgcc tggcagaaat
     3781 ttattcagca attaaatacc tggaacatgg aggatgtcga tcaattacgc tggccgctgt
     3841 gtgcaatcgc tatagaagcg cgatatcttt cattaaattg tgcttctttg ctggcagagc
     3901 gtttgaactg gcattcgttt aatgacagcg aaggaatgga tgaggaagaa agggaggctt
     3961 ttcttgaggc cattcaggct ggtgattgtt tcgatttcct tagccttctg gaatatcccg
     4021 tcgcgttaca gaaccagact gttgaatatt acttcgcgct ggaacgttgc tgccgttacc
     4081 atcctgacta tgtcactgcg tttttggcga tggaaggtcc gtggttcatt cctgatgatg
     4141 caaagttaca tcgcaaactg ttgcgctggt acagctcggt gcaaacaggt atggcggaac
     4201 tcattcctgt agccaaacag tggcaagcgg aagaaccaga aagcgaagat gcccggtatt
     4261 accagtgtgc acaacgtttg tactgcggcg agggggaaag cctgcttgct gagctctgcg
     4321 catactggga gagttaccca tctacacaag ctgataattt gttgttgcag tggagcaagc
     4381 gtcattgccc ggattatttc gcgttattag tgatggttat cgaagcgcgg agcatggtag
     4441 atgcgcaagg tcaaccgctg aaatatgttc ctggtgagag cgcccggacg cggctgttat
     4501 gggcggaaat tttacatagc ggaaaattat cgccgttagg tcaatcgttt attgagtcgt
     4561 tattcttcaa acgcaaagca tgggcgtggt ggaaatcgag agtcggtagc gagacagaag
     4621 aagattcacc gttactggat ttgtatcggg tagcggaaca ggtagtactt gaagcgtttc
     4681 cgaaacaaga gatgctggcc cgtcttaata caaggctgga aggcggagat gctcatccat
     4741 tagaggccat tatcacccgg ttgctgttga cgaaagtaaa actcgagccg gaggatgaag
     4801 atgtcgatga gccaacacct gaaaaccatg aagaaaaaaa tgatgagggt gaaaaaccac
     4861 agagcattac cagcattatc aaaatcagtt taacggtgct ggttataggt tatgctctcg
     4921 gcaaaatcgc gatgttgttt agttgacgat gtgttttttg cgccgatgcc ggatgcagca
     4981 tttacgccgc atccggcagc cgtgattatg aggtctcaat ggctaaataa acgctcgccc
     5041 gccgcctgac ctcatcgacg cgggccatat cgtttgactg cagcgccgca tcaaaatcgg
     5101 tgatcacccg gccaatctct tctcgctcgt cacccagcgc ccgcgcccac aactcttcca
     5161 gtttggcttt aaaggtgcga ttgatgagca tatcgcgcgg ataaattttc aatgcggata
     5221 accgcgtccg actctcttca atctgctgcg cgctcaatgt taccgggctg tggttaatca
     5281 ctctggactt aacgctgccg tcttccagaa gcacgtcaac ttcaagcaac ccgttgatat
     5341 cataactaaa gcgaatatca atcgactgat aagccccggt tttcttcaac ggcacatcga
     5401 aggattcaac cagaatgtta tttttaactt tgtggttttc tccctgatag acgttaaccg
     5461 taatagaatc ctgttccggg tgcatggtcg agtaagtttc tacacgcgac accggtactg
     5521 tggtgtttcg ttcaataatt ggcgagaaaa tgccggaaat gccctggcgg ttaacttcaa
     5581 cgcccaacga gtaagggcaa atatcagtga ggatcacctc ttcaatatct tcactgcgta
     5641 agcggcaggc ggcctggatt gctgcgccca gcgcgacaat ggtgctcggg tcgtaacttt
     5701 gatacggtaa tttgccaaac agacgcacgg cgattcgctg caccagcggc atttgtgacg
     5761 cgccgccaac cagcaccaga ctgtcgattt gactcggctt cagacgtgca tcgcgcaacg
     5821 cctgttcaat cggcactcgc aagcgattga gcagcggcaa ccacaaatct tccagttcgt
     5881 tctcgtaaaa ttcgcactcc cgcgtttcat cctggtactg ccagcgaatg tgcaatggcg
     5941 attgattgct acattttgcc gcttccacac aggcgtacaa ggctgccagt tcactctcgt
     6001 taagcgtggt cctggcgaca tccgcgcgtt ttaaaacctc atcgaccagc atatgggtaa
     6061 aatcttcgcc accaagaaag ttgtcgccag cggaggcgtg aacttcaatc accggcgtgg
     6121 cgtactcaag caccgtaacg tcaaacgtgc cgccaccgag atcaaacacc agcgaacggg
     6181 tattttgttg ggtatgcagg ccatacgcca tcgcagctgc tgtgggttca ttaattaagc
     6241 gtaccgcatt taacccggct aactccgctg ctaaacgggt atgcttgcgt tgttcatcgc
     6301 tgaaataagc cggaacggag atcaccacat ctttaatcgg acgttgcaga aattcttcgg
     6361 catcttcttt taatgagcgt aataccaaag aggacagttc tggcgcgtta aatgtgtcgc
     6421 tgcctaaccg ccagttggta ttactgccca ttgcacgttt aaataacgct gccgttttat
     6481 ccggatgcga agtacgccgt gatacagccg gttttccgac taaaatatga ttattttcat
     6541 ccatgctaat tatggatggt gttaaatatt caccgaactt atttggaatt aattgcgcgg
     6601 caccgtcttt ccagacggca attaaactat tggtagtgcc gagatcgata ccaatggcga
     6661 gttctgcatt atccattgca tttatatctt gaaagaaaaa gagataaaca gattaagacc
     6721 acccaggtaa cgcgtggtga tcttaatcaa tgacgtgtgt taagcgtaaa atttcagacg
     6781 atcagccagt aaatcaacaa agccctgacg atcaacatcg accattacag tggcattcgg
     6841 tttattgcca gtcagataat aataatcaac aaccgtcata ccctggctat atttcccctg
     6901 tgtttccacg ccaacccagc gctcaacagt ggtaaacaac tcgggtttca acagccaggc
     6961 gatggtgcat gggtcatgca gcggtgcacc gacaaagccc catttttcgt ctttatgata
     7021 ttcgaggaag aaatccagca gttcggcaac aatggttgaa acagggttgc caatcgcgcg
     7081 gaaacgctcg gtgtcttcaa cgtggatttg tgctttgtga gtaacatcca gaccggccat
     7141 caccactggg atccctgact ggaagacaat ttctgccgct tccgggtcaa cgtaaatatt
     7201 aaattcagcc gcaggcgtcc agttaccaag ccccattgcg ccgcccataa tcacgatacg
     7261 ggcaattttg ctatgcagtt ccggatggct attgagcagc aaggcaacgt tagtttgcgg
     7321 tccggtagac acaatcgtga caggttccgc actttcgcgc agcgttttcg ccatcagctc
     7381 taccgccgta cagttttgcg gtgcgaacgt cggttccggt aatgccgggc catccagacc
     7441 gctttcgccg tgcacattgt ccgcgataat caactcacgc attaacggtt ttaccgcgcc
     7501 gcctgctacc ggaatatcgg tgcgattaag caaggtcagc atacgcagaa cattgcgtaa
     7561 ggttttttct ggtgtctggt ttccggcgga agacgtaatt gctttgacat caagctctgg
     7621 tgaggcgagg gcgagaacta ttgcgatagc gtcgtcatga cctgggtcgc aatctaacag
     7681 aattggcagt gccattgttg ctccttgttg tgtgcttctt tgcgacaagg gtaacgccag
     7741 gatgtaacag atacgagggg cgaaacgata aagcgtgaga tggcgcgcac ttgggtatgc
     7801 gcgccagagt gattaatgca ggattttcgc gaggaagtct ttcgcgcggt ccgatttcgg
     7861 atcatcgaag aaagcgtctt tcggcgagtc ttcgacaatt ttaccctcgt ccataaagat
     7921 cacccgattc gccactttgc gggcaaagcc catttcgtgg gtcaccacca tcatggtcat
     7981 tccttcgttc gccagttcca ccatcacgtc cagcacttcg ttgatcatct ccggatccag
     8041 cgccgatgtc ggttcgtcga acagcatcgc aataggatcc atacacaacg cgcgagcgat
     8101 tgccacacgc tgctgctgac cgccggaaag ctgcgccgga aatttattgg catgtgcgga
     8161 aagtcccacg cgctccagca atttcaatgc tttttcacga gcgggcgctt tatcgcgttt
     8221 aagcactttc acctgcgcca gagtcaggtt ttcaataatc gacaaatggg ggaacaactc
     8281 aaaatgctgg aacaccatcc cgacgcggga acgcagcttt gccagatcgg ttttcttgtc
     8341 attaaccacg ataccatcga cggtgatttc accttgctgc actggttcga ggccgttgac
     8401 ggttttaatc agcgttgatt tgccggaacc agacgggccg caaaccacca ccacttcgcc
     8461 ttttttcact tcggttgagc agtcggtcag cacctgaaag tgaccatacc attttgaaac
     8521 atttttcagg gtaatcatta tgctgtcctt cttttcaagt agctgaccaa caacgacgcg
     8581 ctaagactaa taacgaaata aacaaatccg gcaaatagga tcatctcaac ctgcgtacca
     8641 tcacgctcac caatggttga ggcggtacgg aagaaatcgg ccagagataa cacatacacc
     8701 agtgaggtat cctggaacag tacgatgccc tgagtgagca gaagcggcac catcgcgcgg
     8761 aacgcctgcg gcagaataat cagtttcatc gactgccagt gagtcattcc caacgccagc
     8821 gcggcgctcg actgaccacg agaaatactt tgaataccag cacggataat ctctgaatag
     8881 taggccgctt caaacattga aaacgccacc atcgccgaaa ttaaacggat atcatttttt
     8941 ggtgataatc ccagcacgtt ttgcagaaaa cctggcacga tcaggtaaaa ccacagcaaa
     9001 accataacta aaggaatcga gcggaatacg ttaacgtagg ctttggcaaa ccacgctacg
     9061 ggcgcaaagc tggacaagcg catcaccgcc agcatggtgc cccacaaaat accaatcacg
     9121 actgccgtga cggtgatttt cagcgtgatc accagcccgt cgagcagata tggcagggaa
     9181 gggacaatgg aactccagtc aaactcgtac attatttgcc ccccatgttg ccaggcaggc
     9241 gaactttacg ttcaaccagc gtcattacca gcatgataac cgcgttaatc aacacatacg
     9301 ccagcgtaat agcggtaaac gactcccagg cgtgggctga gtaatcaagc aatttacccg
     9361 cctgcgccgc catatccacc agaccaatag tcgaggcgat ggcggagttt ttcaccaggt
     9421 tcatcatctc ggaagtcatc ggcgggacga taacgcgata agcattaggc agcagtacgt
     9481 atcgataagc ctgcggtagc gtcaggccca tcgccagcgc ggcatttttt tgcccacgcg
     9541 gcaacgactg aatggcggcg cgtacctgtt cgcaaacacg ggcggcggta aacagcccca
     9601 ggcagagcat ggatgaaagg aaaaactgaa tattgggatc cagctcggcc ttaaaccaca
     9661 taccgatttt ctctggcagc aactccggga tcaccagata ccaggtaaag aattgcacaa
     9721 tcaacggcac gttgcggaac agttcgacat acaacgtacc cagaccggag agaaaacggt
     9781 ttggaacggt acgtaaaatg ccgaaaaatg aaccgacgag gaaagcgata atccaggcgc
     9841 agatcgacaa agcgatcgtc acctgaaaac cactccagat ccaaccaaga taggtggtgt
     9901 tgccgaacgg ggcttgttgt aaaaaaatac cccagttcca gtctatagac ataaatctac
     9961 tccagaaaaa agagggtagc agcgttaact gctaccctcg aagattgtta cccagcgtat
    10021 tgcggttttc aggcccgatg gggaacgacc atcaggcgta tagtctgtcc gtgctacgta
    10081 acaatcgaga gggctggaat ttccgcccct ggttcttgta attagttcag tgctttgtca
    10141 ttcggttctt tgaacagtgc tttcatttcg tctgacagtt cgaaattcat gttcaggttt
    10201 ttcggcggaa ttggattttt gaaccattta tcaaaccatt tttccgcttc accggaagtc
    10261 tgcacctgag cgatagtgtc atccatcagc tttttgaact gcggatcatc tttacgcaac
    10321 atgcaaccgt aggcttcctg agactgcggc ttgccgacga tatcccagtt gtctggtttc
    10381 ttcgctttcg cacgttcacc ggccagcaga gcgtcatcca tcataaaggc aacggcacga
    10441 ccgctttcca gggtgcggaa agagtcaccg tgatctttgg cgctgatgat gcgcatattc
    10501 attttttgct cttcattcag tttgttgagc aaaacttcag aggtagtgcc ggaagtgacg
    10561 actacggctt tgcctttcag gtcggcaaaa tctttgatat cgccgccctt tttggtcaac
    10621 aggcgcgtac cgaccacgaa aatagtgtca gagaaagccg cctgtttttg gcgttcgacg
    10681 ttgttggtgg tagaaccaca ttcaaaatcg aaagtgccgt tttgcagcag tggaatacgg
    10741 ttttgtgagg taatcggaat cagttttacc tgcaagtccg gtttgttgag tttctttttc
    10801 actgcttcaa caatggcgtt ggagtaatcc tgcgaataac ccaccacttt ttgctgattg
    10861 tcgtaataag agaaaggtac tgaagattca cggtgaccga cgacaatcac accgtttttg
    10921 gcgattttgt ccagcgtact gcctgctgcc ggggcggcgt catctgcctg tgccagtcct
    10981 gcggaaagcg ccagggcgag gattgctgtg gcaggtttac gtaattgcat atccaactcc
    11041 tttatcttct gcgttaagaa cgcatggata cccgttgtga gtgtttgtgt tgttatcgtc
    11101 tgcaacttta ttgtgcagtg ttgtgcttgt taggtaacat ttagtttggc taaatgtaaa
    11161 gatattgatg ttttattgtt tgtttttgcg aggtgcaccg caccattccg aagcaaaatt
    11221 cttaaaatgc actcttttgg tgctaccgct gatttgtggt ggtgcaaccc ggttgcgtcg
    11281 cggctgattt cattgcttac acagtaaata aagcaactta catgccaaaa aatacttagg
    11341 ggtgaattaa ttacgattgt aatttcaggc tgaaaaagtc atatttcgga cagagttatc
    11401 ttttcggcat gggacgtttg ccgggatgat aactgtacac gggaaaaatg ctttttacct
    11461 gtcttttcaa ggaagaaaca ccttatgtct gcatcttcgg ttaagccgtt aaatgttcaa
    11521 ttacccgcaa taacccttat cctttttgcg ctctgtgttg ggatattttg ttacctcgca
    11581 caatggatga gttatgaaga agtcgatcaa tccgcactca tccatctcgg tgctaacgtt
    11641 gcttctctca cgttgtcggg tgaatcctgg cgcttattta gcagtgtctt tctgcacagt
    11701 agtttttccc atttgctgat gaatatgttt gcactcctgg tggtgggggc agtggcggaa
    11761 cggatactgg gaaaatggcg acttctgatt atttggttat tctccggcgt ctttggtggg
    11821 ctcatcagcg cctgttatgc gttacgcgat agtgatcaga tagtcatcag cgttggggcg
    11881 tccggggcaa ttatgggaat agctggcgct gcgttagcaa cacagcttgc ttcaggtgcg
    11941 ggcacatacc ataaaaacca gcggcgagta ttttctctgt tgggtatggt ggcgctgaca
    12001 ctgttgtatg gtgcccggca agcaggaata gataatgctt gccacattgg cggcctgatt
    12061 gcgggtggcg cgttgggttg gctgagcgcg cgtttatctg ggcaaaaccg actcgttacg
    12121 gaaggcggga ttattgttgc gggcagtctt cttctgaccg gggctatctg gcttgcgcag
    12181 cagcagatgg atgagtcagt tttacaagtc aggcaaagcc tgcgtgaagc gttttatcca
    12241 caggagattg aacaagagcg acggcaaaaa aagcagcagt tagcggagga acgcaacgcc
    12301 ctcagggaaa cattatccgc tccggtaagt cgtgaacagg ccagtggtga tttgctcgct
    12361 gagattgccg atatccatga tatggcgatc agtcgggatg gtaatatgtt gtatgccgca
    12421 attgaaaaca cgaacagcat tgttgttttc gacctcggac aaaagaaaat cctgcatacc
    12481 tttacagcgc ccatagcgaa agaaaagtca gtcaaacatt gtggtggctg taaagatcag
    12541 ggcgtcagat cgctggcatt aagcccggat gaaaagttga tttatgcgac ttcatttgaa
    12601 gcgaatgcgt tatcggtcat taacgtggcg acaggggaga ttattcaatc gattaccacc
    12661 ggtgcacatc ctgacagcct tatcctctcg cgtgatggta caaaagcctg ggtgatgaat
    12721 cgcaccagta atagtgtgtc agcgattgat ctggtaactt atcagcatgt ggcggatatc
    12781 ccgctggaga aatacgacgg gacggggacg agtaataaac ctggtgcctg ggttatggct
    12841 ctttccccgg atgaaaaaat attgttgata cccggtatgg tcagaggtga cattgtacgc
    12901 atcaatacca tcacgcatca gaaagaaagc tatccggcta gtgatgcgcg aggaacgata
    12961 tcggcgatgc gttttcgacc tgaaaacggg gatgtaattt ttgccgacag ccaggggatt
    13021 tcacgtataa gcgtagggga tcaacaagcc agcattatga cgcaatggtg tagcaggagc
    13081 gtttattccg ttgagggtat tagcccggac ggtcagtatt tagcgttggt gtcatatggc
    13141 ctgcaaggtt atgtcatcct gctcaatatt aatgccgggc agattattgg cgtttatcct
    13201 gccagctacg ttaatcacct tcgtttttca gcggatggta gaaaaatatt tgttatggcg
    13261 aagaacgggt tgatccaact ggacaggacg cgctcgcttg atccgcaggc aattattcgt
    13321 catccccaat atggcaatgt ggcttgtatc cctgaaccgt aagaaaaagg ggcatgataa
    13381 ccatgcccca aagctcaatg agaaagtacg ttttgctcat gcaggataca tcaggcacat
    13441 caacgtttac gtgctcgcag actcatcagc acagcggcaa aaccaaacag tgccgtcagc
    13501 acccacagcg gccagttgcc ggtacgtgcg tatggtgtga gtccggtggt cggcgtcaca
    13561 ttagtggtta acacctcgcg ggtgaactgc gggatcatcg cctgaatctc accctgcggg
    13621 ccaatcaccg ccgtaatgcc gttgttggtg ctgcgcaaca gtgggcgcgc cagctccagc
    13681 gcacgcattc gcgccatctg gaagtgttgc catggaccaa tagatttacc aaaccacgca
    13741 tcgttggaga tagtcagcag atagtcggta tccgggcgga agttatcgcg cacttgctcg
    13801 ccaagaatga tctcgtagca aatggccgca gtaagctcaa taccatttac cgacagcggc
    13861 ggctggatat atggcccacg gctgaacgac gacatcggca gatcaaagaa cggtgctaac
    13921 ggacgcagaa tcgactccag cgggacaaac tcgccaaacg gcaccagatg gtttttgtta
    13981 tagcgatcgg ctgattcgta gctgtacggt gcacctttac ccagtgtgat gatggtgttg
    14041 taggtatcgt agcggttctg cttattgaga cgcgcgtcga caatcccggt tacgagcgag
    14101 ctacctttat cgcgcaactc accgtccagt gctttgagga acggttgctg attaatttcc
    14161 agatcggtta tcgccgactc cggccagata atcaacgatg atttgcccat cagcggagcc
    14221 gttgcgttgt agtaaatctt cagcgtatta agaagctgat ctccatccca tttcagtgat
    14281 tgcggaatat cgccctgaac catcgaaacc tgaatggttt tctccggttg tggggtaaac
    14341 cattggatgt aacgcagcgg gaagggtagg gcaaacagca cgacggccac caccagcgga
    14401 cgccagttgc gtttgaccaa cgccagtgcc agcaggccac taaccatcat cagcaggaag
    14461 ttaatggctt ccacgcccat tatcggtgcc agccctttta acgggccatc aatctggcta
    14521 tagccgaact gtaaccacgg gaagcctgtc agtacccaac cgcgcagaaa ctcggtcact
    14581 tgccagagag aaggggcggc aatcgccacg cgcagccagg tggttttcgg ccacagacgc
    14641 gacagcacgc cagcaaacag tccggtatac agcgacaaat acgccgccag cagcaccacc
    14701 aggaagatgt taaccgggcc aggcatcccg ccaaaggtcg cgatgctgac atagacccag
    14761 ttaataccgc tgccaaagag gccaaatccc cagcaaaagc caatagcggc agactggagt
    14821 ggacggcggt taaaggtcaa cgcctgaagc cccatcaacg aaataatcgc cgcaggccag
    14881 acgtcgtaag gagagaaggc cagcgttccg caggcaccga ataataacgc cagcagcagg
    14941 cgaatgcgct ggcgttcaat taatgaggca aaagccatgt agttatctat ccagtttcgg
    15001 ttttattcat ccagcttcgg ctggggtgag tcatccggga ttttgacatg aacctgaata
    15061 atacgccgac tgtcggccat cgccactttg aactggtaac cgtcgatgtc gatagtttcg
    15121 ccacgcgccg gaagatgccc aaatgcctgc atcaccagac caccgatagt gtcaacctct
    15181 tcatcgctaa agtgggtgcc gaacgcttcg ttgaagtctt caatggaagc cagtgcgcgc
    15241 acggtccagg tatgacgact cagctgacgg aagtcgatat catcttcttc gtcatactcg
    15301 tcttcaatct caccaacaat tagttccagg atgtcttcaa tggtcaccag accggaaacc
    15361 ccaccgaatt cgtcaataac gatcgccatg tggtaacgct gagagcgaaa ctctttcagc
    15421 atccggtcta cgcgcttact ttcaggaacg acaaccgcct gacgtaacac tttgtccatg
    15481 ctgaaggctt cagcatcgct gcgcataaac ggcagcaagt ctttcgccat cagaatccct
    15541 tcaatgtgat ctttgtcttc gctaatcacc gggaaacgtg agtgggcgga ctcgatgatg
    15601 acatcaaggc attcgtccag cgtctggttg cgtttcaggg taatcatctg ggagcggggg
    15661 atcatgatgt cgcgaacgcg ttggtctgcg atgtccatca ccccttcgag catatcgcgc
    15721 gtatcttcgt cgataaggtc gttctgcccg gaatcacgga tcagcgccag cagttcgtca
    15781 cggtttttcg gttcaccgtg gaaaagttgg ctgagtaaca gggagaaaaa tcccttcttg
    15841 ttgcttatcg tgtcactact gtgtgaattg tcgtcgctca tggcgtcgta tgggttctca
    15901 tgttagttaa tcaaaacgcc gtcgttaatc accaacggcg gggacgtctg ccagtcaaat
    15961 gcctggcaaa ttattctttc tcggcaatgt acggatcctc atagcccaga gcaagcataa
    16021 tctctgtttc gagggcttcc atttcttctg cttcgtcatc ttcgatgtga tcgtaaccta
    16081 acaaatgcag actgccgtgc accaccatat gcgcccagtg cgcctccagt ggtttgcctt
    16141 gctcctgagc ttccttctca accacctgac ggcagataac cagatcgccc agtagcgaca
    16201 tttccatgcc aggcggcact tcaaacggga aggagagcac gttggtcggc ttatccttac
    16261 cgcgataggt cagattcaga ctgtggcttt cggcggtatc gaccacgcga atagtcactt
    16321 ccgattcttc ctgaaactgc gggatcaccg cattcagcca tgtttgaaac tggctctctt
    16381 ccggtaaccc ggaattatct tcacatgcca gttgtaaatc gaggatcacc tgactcattt
    16441 ttgttcctgt tcttcgcgct tgcgttctgc cgccagcgcc gcttttcgtt tttgttcggc
    16501 ttcttcccag gcttcatagg cgttaacgat acgcgccacc acagggtgac gaaccacgtc
    16561 ttcgctgtgg aagaagttaa agctgatctc ttcgacatcg gccagcactt cgatggcgtg
    16621 acgtaagcct gatttagtat tacgcggcag gtcgatctgt gtgacgtcgc cggtgataac
    16681 cgcttttgag ttaaaaccga tacgggtcag gaacatcttc atctgttcga tggtggtgtt
    16741 ctggctctca tcgagaatga taaacgcgtc gttcagcgta cgaccacgca tataggccag
    16801 cggcgcgact tcaataacgt tgcgctcaat cagtttctca actttctcaa agcccagcat
    16861 ttcaaacagc gcgtcgtaca gtgggcgcag atacgggtct actttctggc ttaaatcgcc
    16921 aggcaggaag cccagtttct caccggcttc gaccgccgga cgagtcagca gaatacggcg
    16981 aatttcctga cgctccaggg catcaactgc tgcagcgact gccaggtagg ttttacccgt
    17041 acccgccggg ccaacgccga aggtaatgtc atggtcgaga atattggcga tgtactgcgc
    17101 ctggtttggc gtgcgcggct taattacgcc gcgtttggtt ttgatattga ccgctttgcc
    17161 gtactccggc acgctctccg cactttgctc cagtactcgc gcttctttaa tcgcaaggtg
    17221 gatctgttcc ggttcgatat cctgaatctg accgcgcatc ggggctgtat cgacatacag
    17281 gctacgcaga atgtctgccg cagcggtgac gcaaatcgga cggccggtca gtttaaagtg
    17341 gttgtcgcgg cgattgatct cgatgccgag acggcgttcg agctgcttga tgttgtcatc
    17401 aaacgggccg cacaggctca acagacgcgc gttgtctgct ggctccaggg tgatttcgcg
    17461 agtgtctatg ttcaaaccgt tcctcttatc tgtatgtcgc cggaagctga acattcaccg
    17521 gcctataagg aaattattca cgccacagga aaaaggcgca agcattgcga taaagatggg
    17581 gataaagaga gaaaaaacaa ggcccaccgg aacggcgggc ctgagaatta cggctgataa
    17641 taacccacgc caaggtcgtt ttctttgcgg gtacgggcaa tcaccgattc cggtgtttct
    17701 gccacgcgca gacccatttc atcttcagta cgcaccactt taccgcgcag agagttcggg
    17761 tagacgtcgg taatttctac atcgacgaat ttaccgatca tatccggtgt gccctcgaag
    17821 ttgaccacgc ggttattttc ggtacgcccg gaaagctcca tgatgctctt acgcgaagtc
    17881 ccttctacca gaatacgctg ggtggtgccg agcatccggc ggctccacgc catcgcttgc
    17941 tgattaatgc gctcttgcag aatatacaga cgctgcttct tctcttcttc cggaacatca
    18001 tccaccatat cggcggcggg cgtccccgga cgtgcagaga agataaagct gtagctcatg
    18061 tcgaaattga cgtcggcaat cagcttcatc gttttctcga agtcttcggt ggtttcgcca
    18121 gggaagccaa caatgaagtc agaactgatc tgaatatccg gacgcgccgc acgcagttta
    18181 cggatgatcg ctttgtactc cagcgccgtg tgagtacggc ccatcaggtt cagaatgcga
    18241 tcggaaccgc tttgtaccgg cagatgcagg aagctcacca gctcaggcgt gtcgcggtac
    18301 acttcgatga tatcgtcggt gaattcgatc ggatggctgg tggtaaagcg aatacgatcg
    18361 atcccgtcga tcgcagcaac cagatgcagc agatcggcaa acgatccggt ggtgccgtcg
    18421 tagttttcac cacgccaggc gttcacgttc tgaccgagca ggttgacttc acgcacgccc
    18481 tgagcagcaa gctgggcaat ctcaaacaga atatcgtcag acggacggct tacctcttca
    18541 ccacgggtgt aaggcaccac gcagtaggta caatatttat tgcagccttc catgatggag
    18601 acaaacgcgg tcggcccttc ggcgcgcggt tccggtagac ggtcaaactt ctcgatttcc
    18661 gggaagctga tatctacaac cgggctgcgg tcgccgcgca cggagttgat catctccggc
    18721 agacggtgca gcgtttgcgg cccaaaaata atatcgacat agtgggcgcg ctggcgaatg
    18781 tgctcgcctt cttgcgatgc cacgcagcca ccgacgccga taatcaggtc tggattcttc
    18841 tcttttaaca gtttccagcg acccaactga tggaagactt tttcctgagc cttctcgcgg
    18901 attgagcagg tgttcagcag cagcacatcc gcttcttccg ccacgtcggt cagttgatag
    18961 ccgtgggtgg catccagcag atcggccatc ttcgatgaat cgtactcgtt catctgacag
    19021 ccccaggttt taatatggag ttttttggtc atcgacttgc tcttgcgaaa tagtagccag
    19081 gaatgcaggg cgcatagtgt aatgctttgc tgccgttgtg accagtatga gcgttatcag
    19141 cccttggggg taaaaatcct gtaaacttaa agcatattgc taacaggatg attgaccatg
    19201 acaaatcaac caacggaaat tgccattgtc ggcggaggaa tggtcggcgg cgcactggcg
    19261 ctggggctgg cacagcacgg attttcggta acggtgatcg agcacgcaga acctgcaccg
    19321 tttgtcgctg atagccaacc ggacgtgcgg atctcggcga tcagcgccgc atcggtatca
    19381 ttgcttaaag ggttaggggt ctgggatgca gtacaggcta tgcgttgcca tccttaccgc
    19441 agactggaaa cgtgggagtg ggaaacggcg catgtgatgt ttgacgccgc tgaacttaag
    19501 ctgccgctgc ttggctatat ggtggaaaac accgtcctgc aacaggcatt gtggcaggcg
    19561 ctggaagctc atccgaaagt aacgttacgt gtgccaggct cgctgattgc attacatcga
    19621 gataatgatc ttcaggagct ggaactgaag ggcggtgaag tgattcgcgc gaagctggtg
    19681 attggtgctg acggcgcaaa ttcgcaggtg cggcagatgg cgggaattgg cgttcatgcc
    19741 tggcagtatg cgcagtcgtg tatgttgatt agcgtacagt gtgagaacga tcccggcgac
    19801 agcacctggc agcaatttac cccggacgga ccgcgtgcgt ttctgccgtt gtttgataac
    19861 tgggcatcgc tggtgtggta tgactctccg gtgcgtattc gccagttgca gaatatgaat
    19921 atggcgcaac tccaggcgga aatcgcgaag catttcccgt cgcgtctggg ttacgttaca
    19981 ccgcttgccg ctggtgcgtt tccgctgacg cgccgacatg cgttgcagta tgtgcagccg
    20041 gggcttgcgc tggtgggcga tgccgcgcat accatccatc cgctggcggg gcagggggtg
    20101 aatcttggtt atcgtgatgt cgatgccctg attgatgttc tggtgaatgc ccgcagctac
    20161 ggtgaagcgt gggccagtta tcctgtcctc aagcgttacc agatgcggcg catggcggat
    20221 aacttcatta tgcaaagtgg tatggatctg ttttatgccg gattcagtaa taatctgccg
    20281 ccgctgcgtt ttgtgcgtaa tcttggtttg atggcggcgg agcgtgctgg cgtgttgaaa
    20341 cgtcaggcgc tgaaatatgc gttagggttg tagccttaca acattgccgg atgcgtgcca
    20401 accgtaggtc ggataagacg cggcagcgtc gcatccgaca ttgaaggata agacgtgtca
    20461 acatcgcatt cgacattgaa tgaacgcaga aaagcaaaaa gctcgccgaa gcgagctttt
    20521 ttaatgtggc tggggtacga ggattcgaac ctcggaatgc cggaatcaga at
//
LOCUS       DAPHDW010000073        20262 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.073, whole genome shotgun sequence.
ACCESSION   DAPHDW010000073 DAPHDW010000000
VERSION     DAPHDW010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20262
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.073"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            95..210
                     /gene="rrf"
                     /locus_tag="RH057_002690"
     rRNA            95..210
                     /gene="rrf"
                     /locus_tag="RH057_002690"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(441..1199)
                     /locus_tag="RH057_002691"
     CDS             complement(441..1199)
                     /locus_tag="RH057_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HDV3129040.1"
                     /translation="MSQILLQPANAMITLENVNKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQAAPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(1207..2310)
                     /locus_tag="RH057_002692"
     CDS             complement(1207..2310)
                     /locus_tag="RH057_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV3129041.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAVWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTNIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPTWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(2320..3501)
                     /locus_tag="RH057_002693"
     CDS             complement(2320..3501)
                     /locus_tag="RH057_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HDV3129042.1"
                     /translation="MSHRRSTVKGSLSFANPTVRAWLFQILAVVAVVGIVGWLFHNTV
                     TNLNNRGITSGFAFLDRGAGFGIVQHLIDYQQGDTYGRVFIVGLLNTLLVSALCIVFA
                     SVLGFFIGLARLSDNWLLRKLSTIYIEIFRNIPPLLQIFFWYFAVLRNLPGPRQAVSA
                     FDLAFLSNRGLYIPSPQLGDGFIAFILAVVMAIVLSVGLFRFNKTYQIKTGQLRRTWP
                     IAAVLIIGLPLLAQWLFGAALHWDVPALRGFNFRGGMVLIPELAALTLALSVYTSAFI
                     AEIIRAGIQAVPYGQHEAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSS
                     LAAAIGYPDMVSLFAGTVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVE
                     R"
     gene            complement(3569..4594)
                     /locus_tag="RH057_002694"
     CDS             complement(3569..4594)
                     /locus_tag="RH057_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3129043.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPGFSYADADGKFSGIDVDVCRGVAAAVFGDDTKVKYTPLTAKERFTALQSGEVDLLS
                     RNTTWTSSRDAGMGMAFTGVTYYDGIGFLTHDKAGLKSAKELDGATVCIQAGTDTELN
                     VADYFKANNMKYTPVTFDRSDESAKALESGRCDTLASDQSQLYALRIKLSNPAEWIVL
                     PEVISKEPLGPAVRRGDDEWFSIVRWTLFAMLNAEEMGINSQNVDEKAANPATPDMAH
                     LLGKEGDYGKDLKLDNKWAYNIIKQVGNYSEIFERNVGSESPLKIKRGQNNLWNNGGI
                     QYAPPVR"
     gene            complement(5025..5246)
                     /locus_tag="RH057_002695"
     CDS             complement(5025..5246)
                     /locus_tag="RH057_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129044.1"
                     /translation="MKRLIPVALLTALLAGCAHDSPCVPVYDDQGRLVHTNTCMKGTT
                     QDNWETAGAIAGGAAAVAGLTMGIIALSK"
     gene            complement(5499..8603)
                     /gene="acrF"
                     /locus_tag="RH057_002696"
     CDS             complement(5499..8603)
                     /gene="acrF"
                     /locus_tag="RH057_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit AcrF"
                     /protein_id="HDV3129045.1"
                     /translation="MANFFIRRPIFAWVLAIILMMAGALAILQLPVAQYPTIAPPAVS
                     VSANYPGADAQTVQDTVTQVIEQNMNGIDNLMYMSSTSDSAGSVTITLTFQSGTDPDI
                     AQVQVQNKLQLATPLLPQEVQQQGISVEKSSSSYLMVAGFVSDNPDTTQDDISDYVAS
                     NVKDTLSRLNGVGDVQLFGAQYAMRIWLDADLLNKYKLTPVDVINQLKVQNDQIAAGQ
                     LGGTPALPGQQLNASIIAQTRLKNPEEFGKVTLRVNSDGSVVRLKDVARVELGGENYN
                     VIARINGKPAAGLGIKLATGANALDTAKAIKAKLAELQPFFPQGMKVLYPYDTTPFVQ
                     LSIHEVVKTLFEAIMLVFLVMYLFLQNMRATLIPTIAVPVVLLGTFAILAAFGYSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMMEDKLPPKEATEKSMSQIQGALVGIAMVLS
                     AVFIPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATLLKPTSAEHHENK
                     GGFFGWFNTTFDHSVNHYTNSVGKILGSTGRYLLIYALIVAGMVVLFLRLPSSFLPEE
                     DQGVFLTMIQLPAGATQERTQKVLDQVTDYYLKNEKANVESVFTVNGFSFSGQAQNAG
                     MAFVSLKPWEERNSDENSAEAVIHRAKMELGKIRDGFVIPFNMPAIVELGTATGFDFE
                     LIDQAGLGHDALTQARNQLLGMAAQHPASLVSVRPNGLEDTAQFKLEVDQEKAQALGV
                     SLSDINQTISTALGGTYVNDFIDRGRVKKVYVQADAKFRMLPEDVDKLYVRSANGEMV
                     PFSAFTTSHWVYGSPRLERYNGLPSMEIQGEAAPGTSSGDAMALMENLASKLPAGIGY
                     DWTGMSYQERLSGNQAPALVAISFVVVFLCLAALYESWSIPVSVMLVVPLGIVGVLLA
                     ATLFNQKNDVYFMVGLLTTIGLSAKNAILIVEFAKDLMEKEGKGVVEATLMAVRMRLR
                     PILMTSLAFILGVLPLAISNGAGSGAQNAVGIGVMGGMVSATLLAIFFVPVFFVVIRR
                     CFKG"
     gene            complement(8615..9772)
                     /gene="acrE"
                     /locus_tag="RH057_002697"
     CDS             complement(8615..9772)
                     /gene="acrE"
                     /locus_tag="RH057_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrE"
                     /protein_id="HDV3129046.1"
                     /translation="MTKHARFFLLPSFILISAALIAGCNDKGEEKAHVGEPQVTVHIV
                     KTAPLEVKTELPGRTNAYRIAEVRPQVSGIVLNRNFTEGSDVQAGQSLYQIDPATYQA
                     NYDSAKGELAKSEAAAAIAHLTVKRYVPLVGTKYISQQEYDQAIADARQADAAVIAAK
                     ATVESARINLAYTKVTAPISGRIGKSTVTEGALVTNGQTTELATVQQLDPIYVDVTQS
                     SNDFMRLKQSVEQGNLHKENATSNVELVMENGQTYPLKGTLQFSDVTVDESTGSITLR
                     AVFPNPQHTLLPGMFVRARIDEGVQPDAILIPQQGVSRTPRGDATVLIVNDKSQVEAR
                     PVVASQAIGDKWLISEGLKSGDQVIVSGLQKARPGEQVKATTDTPADTASK"
     gene            10171..10833
                     /gene="acrS"
                     /locus_tag="RH057_002698"
     CDS             10171..10833
                     /gene="acrS"
                     /locus_tag="RH057_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrS"
                     /protein_id="HDV3129047.1"
                     /translation="MAKRTKAEALKTRQELIETAIAQFAQHGVSKTTLNDIADAANVT
                     RGAIYWHFENKTQLFNEMWLQQPSLRELIQEHLTAGLEHDPFQQLREKLIVGLQYIAK
                     IPRQQALLKILYHKCEFNDEMLAEGVIREKMGFNPQTLREVLQACQQQGCVANNLDLD
                     VVMIIINGAFSGIVQNWLMNMAGYDLYKQAPALVDNVLRMFMPDENITKLIHQTNELS
                     VM"
     gene            complement(10836..11015)
                     /locus_tag="RH057_002699"
     CDS             complement(10836..11015)
                     /locus_tag="RH057_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HDV3129048.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLATETDKCRNMNSVNP
                     LKLVNCDELNFQDRM"
     gene            complement(11099..11983)
                     /gene="yhdJ"
                     /locus_tag="RH057_002700"
     CDS             complement(11099..11983)
                     /gene="yhdJ"
                     /locus_tag="RH057_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417728.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HDV3129049.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDALAELKKIPAESVDLIFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPEALLKRIILASSNPGDIV
                     LDPFAGSFTTGAVAVSSGRKFIGIEINSEYIKMGLRRLDVASHYSAEELAKVKKRKTG
                     NLSKRSRLSEVDPDLIAK"
     gene            complement(12069..12365)
                     /gene="fis"
                     /locus_tag="RH057_002701"
     CDS             complement(12069..12365)
                     /gene="fis"
                     /locus_tag="RH057_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HDV3129050.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(12391..13356)
                     /gene="dusB"
                     /locus_tag="RH057_002702"
     CDS             complement(12391..13356)
                     /gene="dusB"
                     /locus_tag="RH057_002702"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HDV3129051.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSDPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGEMLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(13685..14566)
                     /gene="prmA"
                     /locus_tag="RH057_002703"
     CDS             complement(13685..14566)
                     /gene="prmA"
                     /locus_tag="RH057_002703"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HDV3129052.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(14578..16029)
                     /gene="panF"
                     /locus_tag="RH057_002704"
     CDS             complement(14578..16029)
                     /gene="panF"
                     /locus_tag="RH057_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HDV3129053.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIVVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(16019..16261)
                     /locus_tag="RH057_002705"
     CDS             complement(16019..16261)
                     /locus_tag="RH057_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HDV3129054.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVAAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(16370..17719)
                     /gene="accC"
                     /locus_tag="RH057_002706"
     CDS             complement(16370..17719)
                     /gene="accC"
                     /locus_tag="RH057_002706"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HDV3129055.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSADRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKADTIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFNNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(17730..18200)
                     /gene="accB"
                     /locus_tag="RH057_002707"
                     /gene_synonym="fabE"
     CDS             complement(17730..18200)
                     /gene="accB"
                     /locus_tag="RH057_002707"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HDV3129056.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(19178..20152)
                     /locus_tag="RH057_002708"
     CDS             complement(19178..20152)
                     /locus_tag="RH057_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HDV3129057.1"
                     /translation="MQALLLEQQDGKTLASVQTLDESRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASRILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
BASE COUNT         5252 a         5223 c         4893 g         4894 t
ORIGIN      
        1 gagcgcaccc ttggtaaggg tgaggtcccc agttcgactc tgggtatcag caccactttt
       61 taggttaaag ttcggcagat tagaaaagaa tttgtctggc ggcagtagcg cggtggtccc
      121 acctgacccc atgccgaact cagaagtgaa acgccgtagc gccgatggta gtgtggggtc
      181 tccccatgcg agagtaggga actgccagac atcaaataaa acaaaaggct cagtcggaag
      241 actgggcctt ttgttttatc tgttgtttgt cggtgaacac tctcccaacc aggacaaatc
      301 cgccgggagc ggatttgaac gttgcgaagc aacggcccgg agggtggcgg gcaggacgcc
      361 cgccataaac tgccagacat caaatcaagc gaaaggccat ccgaaaggat ggcctttttg
      421 cttttcgaac taacattcaa ttaatggatt acctgcgata aaaatgccct cgtacgctct
      481 gatttaggat gcgcaaaaaa ttcatcaggt gcagcttgtt ccactatctc cccacgatcc
      541 ataaaaatta cccggtcagc gacggtacgt gcaaacccca tctcatgtgt tacacacaac
      601 attgtcatac ccgactgcgc cagcccaatc atcgtatcca gcacctcttt caccatctcc
      661 ggatcgagcg ccgacgttgg ctcatcaaac aacataattt tcggcttcat acacagcgaa
      721 cgcgcaatgg caacgcgttg ctgctgacca ccagaaattt gtccgggaaa cttatgcgca
      781 tgttcggcaa ttctcacccg ctcaaggtaa tgcatcgcca gagcttcagc ctctttcttt
      841 ggcatcttgc gtacccaaat cggtgccagg gtacagttct gtagaacggt cagatgaggg
      901 aagagattga aatgctgaaa gaccattccc acttcctgcc ggacgcgctc aatattgcgg
      961 atatcttcat taagttcgat gccatctacc acgatccgtc cctgttgatg ttcttccaga
     1021 tgattaatac aacgaatggt tgtcgattta ccggaacctg aagggccaca cagaacgatc
     1081 cgttctcccg gttgcacggt taaatttata tttttcaaaa catggaattg tccataccat
     1141 ttattgacgt tttccagcgt aatcatcgcg ttagcaggtt gcagtaaaat ttggctcata
     1201 gtgtcctcaa tgcggtgtac gcccggtgtt aaaacgtttt tccagatgct ggctatagcg
     1261 cgacatgctg aaacagaaga tccagtagat cagcgcggcg aaaacatacc cttccgtcga
     1321 catacccaac caggtgggat caacggttgc ctgctgaacg ctactgaaaa gatcgaacaa
     1381 cccgataatg atcaccaggc tggtatcttt gaagagtgcg atgatggtat ttaccagccc
     1441 aggaattacc agcttcaacg cctgtggcag aataaccagc ccctgagttt tccagtaacc
     1501 caacgccagc gactctgccg cttcatattg ccctttaggc agcgcctgta atccgcctcg
     1561 cacgacttcc gcaacatatg ctgactgaaa caggatcacg ccaaccagcg cgcggatcag
     1621 tttgtcgata ttggtgcctt ctgccataaa caaaggcagc atgaccgaag acataaacaa
     1681 tacggtaatc agcggtacgc cgcgccagaa ctcgataaaa atgaccgata agatacgcac
     1741 aatcggcata tgggagcggc gacctaacgc cagtaagatc ccccacggta gcgcccccgc
     1801 aatcccaacg gatgcaataa ttaaagttag cgtcagcccg ccccattgcc gggtttcaac
     1861 ccgctccaga ccaaaaaaac cgccatacat cagccaccag acaatcagtg ggtaaatcac
     1921 cgcccagacc gcaatatagc gaccgcgatg cgggagtatt ttccagaaca ttggtgcgat
     1981 tgacacaagg ccaatcagta atgccaggtt aattcgccag cgttggtcgt gtgggtaaag
     2041 tccatacata aactgaccaa atcgttcgtg gatgaagacc caacaggcac cggcttttgt
     2101 gcagtcggca cgcgttgagc caacccaatt agcctgcaaa aatgcccagt tcagcaacgg
     2161 tgggatcagt tcccacatca accatatgca gccaatagtc agcaggctat tgctccagct
     2221 ggagaacaga ttttttcgca cccacaccat cgcgcggctt gagttatggc tcgctgggcg
     2281 cgagggctga gacagcaata cttttgtcat catgactcct tagcgttcaa cgatcgcgat
     2341 gcggcggtta tagatattca tcagcagcga gatagtcagg ctgataatca gatagaccga
     2401 catggtcatg gctatcgtct cgatggcttg ccccgtctgg ttcagcacgg tgccagcaaa
     2461 cagcgaaacc atatcgggat agccaatagc ggcggcaaga gaggagtttt tgacgatgtt
     2521 gagatactgg ctggttaacg gcggaataat cactcgcagt gcctggggaa taatgacctg
     2581 gcgtagcgta accgggttgg gtaatcccag tgaccgagcc gcttcatgtt gaccataagg
     2641 cactgcctgg atcccagcgc ggataatctc ggcgataaat gcagatgtat aaaccgaaag
     2701 tgccagcgtt aaggctgcca gttcaggaat taaaaccatc ccgccgcgga agttaaagcc
     2761 tcgtagggct gggacatccc agtgtagtgc tgcgccaaaa agccattgcg ccagtaaagg
     2821 caaaccaatg atcaacactg cggcgatcgg ccaggtgcgg cgcagttgtc cggtctttat
     2881 ctggtatgtt ttattaaagc ggaatagccc aacagaaagg actatagcca taacaacagc
     2941 caggataaac gcaataaatc cgtctcccag ctgcggtgac ggaatataaa gcccacgatt
     3001 gctcaaaaag gccagatcaa acgcgctcac agcctggcgt ggtccgggca aattgcgcaa
     3061 cacggcaaag taccagaaga agatttgcag cagcggggga atattacgga agatctcaat
     3121 ataaattgtg gatagttttc gtagcagcca gttatccgaa agtctcgcca gaccgataaa
     3181 gaagcctaaa acagaagcga acacaataca caatgcagaa accagtagcg tattgagtaa
     3241 gccgacaata aaaacgcgtc cgtaggtgtc gccctgctgg taatcgatca aatgctggac
     3301 aataccgaag ccagcgccgc gatccagaaa ggcaaaacct gaagtaatgc cacgattatt
     3361 gagattcgtt acagtgttgt gaaataacca accaacaatg ccgacaacag caacaacggc
     3421 aaggatctgg aataaccagg cgcgaaccgt agggttggca aaagagagtg agcctttaac
     3481 ggttgagcgg cgatgagaca tagaaacctc ggaaagcata acgctgaaga gagcgccgcc
     3541 gtagcgacgc ccgttacctc acaccgactt aacgcacggg cggcgcgtac tgaataccgc
     3601 cgttattcca gagattattt tgcccacgtt taattttcag cgggctttct gaacctacgt
     3661 tacgctcgaa aatttccgag tagttaccca cctgtttgat gatgttatag gcccatttat
     3721 tatccagctt cagatccttg ccgtaatcgc cttctttacc cagcagatgt gccatatcag
     3781 gcgttgctgg attagccgct ttttcatcga cgttctggga attgatgccc atctcttcag
     3841 cattcagcat ggcgaaaagc gtccagcgta caatcgagaa ccattcatca tcgccacgac
     3901 gaactgccgg cccaagcggt tctttagaga taacctccgg taagacaatc cattcagctg
     3961 ggttgcttaa tttgatgcgc agggcataca gttgcgactg atccgaggcc agcgtatcgc
     4021 agcgaccaga ttccagtgcc ttcgctgatt cgtcagagcg atcgaaagtc accggtgtgt
     4081 acttcatatt gtttgccttg aaatagtcgg caacgttgag ttcggtatca gtacccgcct
     4141 gaatacagac ggtagcgcca tccagttctt tcgcgctttt tagccccgct ttatcgtgcg
     4201 tcaggaagcc aatgccgtcg taataagtga cgccggtaaa tgccattccc atcccggcat
     4261 cgcgagatga agtccaggtc gtattacggg agagcaaatc cacttcccct gactgtaaag
     4321 cggtgaagcg ttcttttgct gtgagtgggg tatatttcac tttcgtgtcg tcaccaaata
     4381 cagcagcggc aacaccacga caaacatcaa catcaatacc tgaaaactta ccgtctgcat
     4441 cggcataaga gaacccaggt aatccatcac tgatcccgca ttgcacaaaa ccttttttct
     4501 gaacggcatc aagcgtcgcg ccagcatgcg cctgatttgc aacggcaagc agcacgctgg
     4561 cggcagccag tgtggctatc atcatctttt tcataatgct tcctgtgagg cgaaaattat
     4621 cgttatagga cgtttgggaa acgtctttta cctgtctgtg gccatcctct tataagcaaa
     4681 agtagtgcca gcttttgcgc ccgatttcgc gtcgcaataa gctgagtgta gacaggaaat
     4741 aataaaaaca gcgccccgca atggtgcgaa aatgaaacat gcgccacatt ttggcgcaaa
     4801 atatttacgc gtgttttact aagcagaata atgattgtta gcgtgagtat atttctggtg
     4861 ggaagtgtga aagttaaatt gatgcgttat ttcgaaagca gcgaaataac gcgatataac
     4921 atgctaaatc agcagagaat tagccagcag aatatagcaa tattttatct tatgtaaaga
     4981 tactgtatcg ttaaacagag cgcgaaactc cgcgccctgt tcagttattt cgacagagcg
     5041 atgatcccca tcgtcaggcc agcaacagcc gctgccccgc cagcaatagc cccagccgtt
     5101 tcccagttat cctgagtcgt gcctttcata caggtattgg tgtgaaccag acgaccctga
     5161 tcgtcataaa ccggaacgca gggcgaatcg tgagcacagc ccgccagcaa tgcggtgagc
     5221 aatgccacag gaattaatct tttcatggtg ttacctcagt aataaacgct aacgcttaat
     5281 aagttaactc tcttattaac gagctgaatg ccattttatc accggaggtt ataaatcttg
     5341 cggaatgaat aatgacaaat tatgtgaatt gtaaaaatag tgaagggagc gggaactata
     5401 gaaagctttt aaagagtgag cagaagaata tcttattaat ttttatgggt ttcactggaa
     5461 ataataaagg cacccgaaag cgcctttatg tttctgattt atcctttaaa gcaacggcgg
     5521 atcaccacaa agaacaccgg tacgaagaag attgccagca acgttgcaga gaccattcct
     5581 cccattaccc cgatacccac agcgttctgc gcgccactgc cggcaccgtt actgatagct
     5641 agcggtaata cgccgagaat aaaggcgaga gaggtcatca ggataggacg cagacgcata
     5701 cgtactgcca tcagtgtcgc ttcaacaaca cctttaccct ctttctccat gagatcttta
     5761 gcgaactcaa caatcaaaat agcgtttttg gccgacaagc caattgtcgt tagcaagccc
     5821 accataaagt agacgtcatt tttttgatta aagagtgttg ccgccagtag cacgccgaca
     5881 atccctaacg gcactaccaa cataaccgag acaggaattg accagctttc atagagtgca
     5941 gcaaggcaca ggaaaacaac cacaaaggaa attgctacca gagcgggagc ctggtttccc
     6001 gataagcgtt cctgatacga catacccgtc cagtcataac caatgcccgc aggtaatttt
     6061 gacgcaaggt tttccatcaa cgccatggca tcgccggaac tggttcctgg cgcggcttcc
     6121 ccctgaatct ccattgacgg cagaccgttg tagcgttcca gtcgcggaga gccatacacc
     6181 caatgtgaag tggtaaaggc cgagaatggc accatttcgc cgttggcgct gcggacataa
     6241 agtttatcga catcttctgg cagcatacgg aatttggcat ccgcctgaac atacaccttt
     6301 ttcacgcgac cacggtcgat gaagtcgtta acgtaagtcc cacccaacgc cgttgaaatg
     6361 gtctgattga tgtcagaaag tgaaacacct aatgcctgcg ccttttcctg gtcaacttcc
     6421 agtttaaact gcgcggtgtc ttccaggcca ttaggacgca cgctgactaa gctggcagga
     6481 tgttgcgccg ccatgccaag caactggtta cgggcctggg tcagggcatc gtgacccagc
     6541 ccagcctgat caattaactc aaagtcgaaa cccgttgccg tgcccagttc aacaatggct
     6601 ggcatattga atggaatgac aaaaccgtcg cggatcttgc ccaattccat tttggcacga
     6661 tggattaccg cttccgcact gttttcatca ctattacgct cttcccacgg tttcagactg
     6721 acgaaggcca taccggcgtt ttgtgcctgg ccgctgaagc taaagccgtt aaccgtaaag
     6781 acactttcaa cgttcgcttt ctcgttcttc agatagtaat ccgtcacttg atccaacact
     6841 ttttgcgtcc gctcttgcgt cgcgccagcg ggtaactgaa tcatggtcag aaagacaccc
     6901 tgatcctctt caggtaagaa ggaagacgga agacgtaaaa acaacaccac cattcctgca
     6961 acaatcagcg catagatcag taaatatcgt cctgtggaac cgaggatttt gccgacgctg
     7021 ttggtgtagt ggttaacgct atgatcgaag gtggtattaa accagccgaa gaaaccaccc
     7081 ttattttcgt gatgctcagc tgaggtgggt ttgagcagtg ttgcacataa cgcaggggta
     7141 agaatcaatg ccaccagaac agaaagcgcc attgccgaaa cgatggtgat agagaactgg
     7201 cgataaattg ccccagtaga accgccgaag aatgccatcg gaataaatac cgctgacagc
     7261 accatcgcga tacccaccag tgcgccctga atttgcgaca tcgatttttc cgtcgcttct
     7321 tttggcggga gcttatcctc catcatcacg cgctcgacgt tctccaccac cactatcgca
     7381 tcatcgacga gcagcccgat ggcaagcacc atcccgaaca tcgttagtgt gttgatggag
     7441 taaccaaaag cggcgaggat ggcaaacgtc cctaataaca ccacgggtac cgcaatggtg
     7501 gggatcagcg ttgctcgcat attctgcaag aacagataca tcaccaggaa caccagcata
     7561 atggcttcga acagcgtttt taccacttcg tgaatagaaa gctggacgaa tggcgtggtg
     7621 tcataagggt agagaacctt cattccctgc gggaagaatg gctgtaattc cgccagtttt
     7681 gccttaatgg ctttcgcggt atcgagagca ttcgcgccgg ttgccagctt aatccccagg
     7741 cccgccgccg gttttccgtt gatacgagcg ataacgttat agttttcacc gccaagttca
     7801 actcgtgcga catctttcag gcgtaccacc gagccgtcac tgtttacgcg cagggttact
     7861 ttgccgaatt cttccggatt ttttaaccgc gtctgagcaa taattgaggc gttcaactgt
     7921 tgccctggta acgctggcgt tccgcccaac tgtccggcag cgatctgatc gttctgtacc
     7981 ttcaactggt taatcacatc aaccggtgtc agtttatatt tgtttagcag atcggcgtcc
     8041 agccagatac gcatcgcata ctgtgcgccg aaaagctgta cgtcaccgac gccattcaga
     8101 cggctaagcg tatctttaac gttagaggcc acatagtccg agatatcgtc ctgtgtggtg
     8161 tctgggttat cagagacaaa gcccgccacc atcaaatagc tgctactgga cttttcaaca
     8221 ctgatcccct gctgctgaac ctcctgcggc agcaacggcg tggcgagctg gagtttgttc
     8281 tgcacctgca cttgcgcgat atcaggatcg gtcccggact ggaaggtaag ggtaattgtc
     8341 acgctaccgg cggaatcgct ggtggaggac atatacatca ggttatcgat accgttcata
     8401 ttctgttcga taacctgcgt caccgtatcc tgcacggtct gcgcatcagc gcccggatag
     8461 tttgctgaaa cagaaaccgc aggcggtgca atggttggat actgagcgac gggtaattgt
     8521 aggatcgcca gtgcgcccgc catcatcaga ataatggcca gcacccatgc aaatatcggt
     8581 cgtcgaataa aaaagtttgc catgtcagat taccttactt cgatgcagta tctgcggggg
     8641 tatcggtagt ggctttaacc tgctctcccg gacgcgcttt ttgcaggccg ctgacaatga
     8701 cttgatcgcc agatttcaga ccttcgctaa tcaaccattt atcgccaatc gcctgactgg
     8761 caacgacagg gcgcgcttcc acctgacttt tatcgttaac aatcagcacg gttgcatcac
     8821 cacgcggtgt gcggctaacg ccttgttgcg ggataagaat ggcgtcaggt tggacgcctt
     8881 catcaatccg tgcgcgcaca aacatacccg gcaaaagcgt atgttgcggg ttagggaaga
     8941 cggcacgtag ggttatggag ccggtgcttt catcaacggt cacatcagag aattgcagcg
     9001 tacctttcag gggataggtt tgaccgtttt ccatgaccaa ctctacgttg ctggtggcgt
     9061 tttccttatg caaatttcct tgctctacgg attgcttcag cctcataaaa tcgttgctgg
     9121 attgggtcac atcaacgtag ataggatcga gctgctggac agtcgccagt tcagtcgttt
     9181 gcccattagt gacaagagcg ccttcggtca cggtcgattt gccgatacgt ccgctaattg
     9241 gcgcagtcac tttggtataa gcgagattga tgcgagcgct ttcgactgtg gcttttgcgg
     9301 caatcacggc ggcatcggcc tggcgagcat cagcaatggc ctggtcgtac tcctgctggc
     9361 tgatgtattt cgtacccacg agcggaacgt aacgttttac cgtcaaatgc gcgatggcgg
     9421 cggcggcttc acttttcgcc agttcgcctt tcgcgctgtc ataatttgcc tgataggtcg
     9481 cgggatcgat ctggtacagg gactggcctg cttgtacatc gctgccttca gtgaaattac
     9541 gattcagtac gatcccgcta acctgtgggc gaacttcggc tatacggtaa gcattggtgc
     9601 ggcctggtaa ttcagtctta acttctaacg gggccgtttt cacaatatga acggtaacct
     9661 gcggttcacc gacgtgagct ttctcttctc ccttatcgtt acaaccggcg attaaagccg
     9721 cggagatcag aataaaggag ggcaggagga aaaacctggc atgtttcgtc attactattc
     9781 ctcaaaaaac caaaagcgcg ttatttaccc agaaaggcaa cgcgttaact cgcagaaaga
     9841 aaaatacagt ttgctatcct acaaattatc attcgtcgat gtaaggaata gttatgaata
     9901 ctggcgtctc aaggcacata aacacaaaaa aagattaata ttctactgtt ttattttgac
     9961 gcgggttgaa agaggcagaa ttaaaacctc gtaaattgaa atatatattc atgtggcgaa
    10021 tgtattttag gtaaataata tatattattt atttgacgat ttatagcaac tcaatattag
    10081 cctctcgtat aaatacacat taggtgatag attaaccttc gctattttct cactctgtgt
    10141 cgaatatatt tatttcctga ataattaatc atggcaaaaa gaaccaaagc cgaagctctg
    10201 aagacccggc aagaactgat tgaaactgcc atcgcccagt ttgcgcagca tggcgtaagc
    10261 aagacgacac tcaacgacat tgccgacgct gctaacgtta cgcgtggcgc tatctactgg
    10321 cacttcgaaa acaagactca actgtttaat gagatgtggt tgcaacagcc ttcattgcgg
    10381 gagttaatcc aggaacactt gacggctgga ttagagcatg acccgtttca acaattgcgt
    10441 gaaaaattga ttgtcggctt gcaatatatt gccaaaattc cccgccagca ggcgttgctg
    10501 aaaatcttat atcacaaatg tgaatttaat gatgagatgc tggccgaggg agtgatacgc
    10561 gaaaagatgg gcttcaatcc gcagactctc cgcgaagtat tgcaggcgtg tcagcaacaa
    10621 ggttgtgtag caaataacct cgatttagat gttgtgatga ttattattaa tggtgccttc
    10681 agcggaattg ttcaaaactg gttaatgaat atggcgggtt atgatcttta taaacaagcc
    10741 cccgctctgg tcgataacgt attaagaatg ttcatgccag atgaaaacat aacgaaatta
    10801 attcatcaaa cgaatgaatt aagtgtcatg taaattcaca ttctgtcctg aaaattcagt
    10861 tcgtcacagt taaccagttt tagtggatta actgagttca tattgcggca tttgtctgtt
    10921 tctgttgcca gcagttcaat ccactgcatc agtaaagtat caaacagaaa aacgaagaca
    10981 gcgaaaacga cgagccacca atacttgcga atcattacct gagcctgtgt atgtttaacc
    11041 aagtctggca ggaaatatac acgaaatgaa taatctgaaa accaggctta cacttccttt
    11101 actttgcaat gagatcgggg tcaacttcgc ttaaccggct tcgttttgac aggttgccgg
    11161 tctttctttt tttcactttc gccagttctt ccgcagagta atgcgacgcg acatccagcc
    11221 gtcgaagccc cattttgatg tactcgctgt tgatctcaat accaatgaat tttcgcccgc
    11281 tggagacggc tacggcaccg gtagtaaagc taccggcaaa cgggtcgaga acgatatcgc
    11341 ctgggttgga agaggcgaga ataatgcgtt tcagtaaggc ttccggtttt tgcgtcgggt
    11401 ggttctcata ttcatccatc aaataacgca cgcgcggaaa atcccagacg ttacccggta
    11461 ctttttgatg attgtatggc tgtggaggat ttttgcgata atcgatcaac gcgcgctgcg
    11521 atccggtttt ggcttctacc agaatagcat caccgttgaa tgtgtagttc tttgcgtctt
    11581 tcaccatcat caggatgggt tcgtacatgg agccgtagtg ttttttcgcc tgtactccag
    11641 aactgtcata tgaccagacg atgcgacttt tgatggtaaa aagcttgcgg cactggagat
    11701 cgataaaggg catgttttcc gtgctgttca tgatgtacat gctgccctgc tttttcagaa
    11761 cgcggtggca ctctgcaatc acttcaaaca gccagtcgat aaacagatct tctttccagg
    11821 cttcgatcag accatcaaaa tttttaccga tgttatatgg tgggtcggca aagatcagat
    11881 cgacactttc ggcggggatc tttttaagtt cggcaagcgc atcaccgtga ataatggtct
    11941 tagcttcatt accaaaccgg gtcggttcac atcctgttct catggtcact ccctttgtga
    12001 cacctataaa aaaggcgctt ccccatgccg agtagcgcct ttttaatcaa gcatttagct
    12061 aacctgaatt agttcatgcc gtattttttc aattttttac gcagcgtacc acggttgatg
    12121 cccatcatca gcgcagcacg ggtctggtta ccacgggtgt attgcatcac catgtccaac
    12181 aggggctgtt ctacttcagc cagtaccagc tcatagaggt cattcacatc ctgaccattc
    12241 agttgagcaa aatagttctt cagtgcctgt ttaaccgagt cacgcagggg tttttgggtt
    12301 acctgatcct gagagttaac ggtagaaacg gtcagtacgt cagaatttac gcgttgttcg
    12361 aacatagttc tgtcagctct ttatttctgt ttacgcaaaa ttttcgaagt atgcctccaa
    12421 cgcctccagc tgttcgctgg catcctcaat ggcgttgaat gtgcgccgaa actggtcatt
    12481 tggagcgtgt tcctggagat accaggaaac gtgtttacgt gcaattcggt acccttttgc
    12541 cggaccataa aagtcatgca gttcccgaac gtgcgcgcaa agcaagcgct taacctctgc
    12601 caaaggcagc gggggcagca tctccccagt gtccagataa tgctggattt cccgaaagat
    12661 ccagggtctt ccctgagctg cgcggcctat catcagggca tccgcccctg tatagtcgag
    12721 cacagctctg gctttaagcg ggtcagtaat gtcgccattc gcgataaccg gaatggaaac
    12781 tttctgctta actgcccgaa tactgtcgta ctcagcttct ccattgaaca aacaggcgcg
    12841 tgtacggcca tgaatggtca gagcctgaat gccacagtct tcagccagtt gggcaatctc
    12901 ttcgcagtta cggtgttccg gtgcccagcc ggtgcgaatc ttcagggtaa caggaacgtc
    12961 cactgcattg acgacctcgg taaggatcga tttaacgaca tccgggtact gcaagagggc
    13021 tgaacctgcg agcttgcgat tcactttttt agccgggcaa cccatattga tatcaataat
    13081 ctgggcaccg ctttccacgt taatacgtgc tgcatctgcc atttctttcg gatcgctacc
    13141 agcaatttgc acggtgcgaa taccgggttc atcaatgtgc accatccgta aacgagattt
    13201 gtcgctttcc caaacctgtg ggttagaaga catcatctcg gatactgtca atccggctcc
    13261 catctcgtag cacaacgtcc gaaaaggtct gtctgtaatg ccagccatgg gcgctgcgat
    13321 caggcgattt ctgagctgat attgtccgat gcgcatgagt taagaaatga ccatactgtg
    13381 actgcaaggc ggcgtatatt acgcattttt tgcacgagat gaaaggccaa actttgacca
    13441 atcctctgag atggatcaaa gaattgcatt taaaatgagc gtggtgcgat aattactcat
    13501 aaaaatcatc atattagaaa atagtgacta aaatttacac tcaaagaaat ttgagtaagt
    13561 tctcaatttt tctttatgaa tgaaaatttt ggcacgcaaa tttgcgtaaa taaccggcaa
    13621 atttacgtgc cttttgtgag cttgctcgca cttcgccccg cgtcacccta cggcgatgcg
    13681 aaggttaatt cttacgaccg gtaatacggc accactcttc tttttccacg accgggtcca
    13741 gtgcgaagct atcggcataa gcttcacaaa cgctctctgc ctggcttgcc agaataccgg
    13801 aaaggcccag caaaccgcct gaaaccggca ggacgctgat taacggtgcc agttcacgta
    13861 atgggcctgc aaggatgtta gcgaccacca cgtcggcttt catttcttct ggctgatctt
    13921 tcggtaagta gagttccaga cggtcagaaa cgccattacg ttcggcgttg tcgcggctgg
    13981 cctgaatcgc ctgcggatcg atatcaatac caatggcttt tgctgcaccc agtttcagcg
    14041 ccgcgatcgc cagaatgccg gaaccacagc caaagtcgat gactgtttta ccggttaaat
    14101 cgaggctgtc gagccattgc aggcacagag aggtggttgg atgggtaccc gtaccaaacg
    14161 ccagccctgg atctaacatc acgttgacgg cgttttcgtc cggcacatca cgccagctag
    14221 ggcagatcca cagtcgttca ccaaagcgca tcgggtggaa attatccatc cattcgcgct
    14281 cccagtcttt atcttctagt tgttcgattt tatgcgcgaa gcctgcgccg agcagcggat
    14341 ggttttccag aatcgccacc acgtcgttca tatcggtttc agcgtcgaac agaccaatca
    14401 catcggtgtc gccccacagg cgcgtttcgc ccggcagcgg ctcaaatact ggcgtatcgt
    14461 gggtatcctg aaaagtgata gaaacggcac ccgcttccat cagcgcatcg ctaagatctt
    14521 ccgcgttcgc gccggtggtg ttcagtttca gttggatcca aggcatggca aaactcttta
    14581 tttatcagta gtcaaaacgg tagcttgcgg gacggatgta ccgaaacggt ttccgaccag
    14641 gaaagccagc aaacttagta gtaacgaggg cacaataggg tggaagccca ggtactgaat
    14701 attcagcgtc gcgagtacgg catacagcac gccgccaacg atcatcgcac ttagcgcgcc
    14761 tttggcgttg gcgcgttccc agtaaagacc cagcaccagc ggccacagga aaacggcttc
    14821 cagcccaccg aaggctagca aattcagcca gatgatcatt tctggcggct tccaggcggc
    14881 aagcagcagc aacgcgccga gaactaacgt aattaccgcc gacatccgct tcagacgcgt
    14941 ctcgttttgc atttgatccg gacggatatt cagatagaga tctttaatga tcgtagcgga
    15001 actttgcagc agttgggcgt taattgtcga catgatcgca gccataggtg cagccagaaa
    15061 gatcccggca gcaaaaggtg gcagcacttt taccattaac gtcgggatca ccagatccgg
    15121 tacggtgaga tcggggatca ctgcacgacc taacgctccg gccaggtgca taccgaacat
    15181 caggattgcg actacaatcg taccgatgat gatcccccgg tgtactgctt tgctgtcttt
    15241 ataagagata cagcgcaccg cagtatgcgg caggccaatc acgccaaagc acaccagtac
    15301 ccagaacgac gtcataaagg caggcgacag aatatcgtca gcgccttgtg gcgtaaccag
    15361 ttgcggatcg atggtttgca aggtctgtac tgcgttgctt aagccgccag cggcatgtac
    15421 cacgccaata agcagcacaa cggtgccaat cagcatcaca agcccttgca tggtgtcgtt
    15481 cagcacgctg gcgcgaaagc caccaaaggc ggtatataac gcaatgctga taccaaaaat
    15541 cagcagcccg gtttcataag gaatacccgc cgcggtttcc agcaggcgcg caccgccgat
    15601 aaactgcacg gtcatcgcac caacgaacgc aaccagcaaa ctcaaactcg ccagccacac
    15661 cagaagacga ctctggtagc gggcaaacag catatcgttc agcgtcactg cattgtagcg
    15721 gcgcgcaaga atcgcaaact tcttgccgag aataccgagt gaaagccaga ctgcaggaag
    15781 ctgaatcatc gccagcaata cccagcccag gccgtattta taagcagctc ctggcccgcc
    15841 gataaacgaa ctggcactga tataggtcgc ggtgagcgtc atcgccagca caataccgcc
    15901 catagagcgg ctgccgagga aatactcatt aaggaaggtg ccggtgctcc gtttacgcat
    15961 cgcataaaca gagataccga acaccaccac cagataggcg accagcggta gaattacttc
    16021 aagctgcatc gtcatcctcc agtgggatat cgcgatagat aaatttcacc atcgcccagc
    16081 acagtccaat aaacagtagc ggcgtcagga tgcaggccat ctcaaaccag cgcggaaagc
    16141 cggtaaaacc gggggcaacg ccaggtaagt aagcggctac taaccaaact gccagataca
    16201 aaagggtcag ccccagcgcc cagcgcgcct ctttatgggc ctgaacaaaa cgagtgtcca
    16261 ttttttgtcc ctgatgggtg aagaaagcga ggattgtacc ttatgggggt tgtcgatccc
    16321 cagtaataaa aaaggccgga aaatccggcc ttttgacgct tttgcagtct tatttttcct
    16381 gaagaccgag ttttttctcc agatagtgga tgttagtgcc accatgctgg aagttctcgt
    16441 cattcatgat gcggatctgc agatcaacgt tggttttgat accgtcgatg atcagctcct
    16501 gcagcgcatt cttcatgcga gcaatcgcca cgtcacggtt ttcaccgtag caaatcagct
    16561 taccgatcat tgagtcatag tacggcggta cggtgtagcc cgcgtagata tgagactccc
    16621 aacgcactcc aaaaccgcca ggtgcgtgga aacgggtgat tttgcctggg cttggcagga
    16681 aggtgttcgg atcttcggcg ttgatacgac attccaccgc atggccgcga acgtgaactt
    16741 cttcttgctt gatcgacagc ggttgaccgg cagcgatacg cagctgttct ttgatcaggt
    16801 caacgccggt gatcatttct gtaaccgggt gttctacctg aatacgggtg ttcatttcga
    16861 tgaaatagaa ctcgccgttt tcgaacagga actcgaaagt acctgcaccg cgatagccga
    16921 tatcaacaca cgctttagca caacgttcgc cgatgtagcg acgcagttcc ggtgtaattc
    16981 ccggtgctgg tgcttcttcg accactttct ggtgacgacg ctgcatagag cagtcacgtt
    17041 ccgccagata gatagcgttg ccctgaccgt cagccagtac ctgaatctcg acgtggcgag
    17101 gattttccag gtatttctcc atgtaaacca tatcgttatt gaatgcggct ttcgcttccg
    17161 cacgggtcat ggagatggat tgtgccagtt cagcgtcgcc gcgcactaca cgcataccac
    17221 gaccgccgcc gccgccggag gctttgataa tcaccggata acctatgcgt ttagcaatgg
    17281 cacggttttt atccatatcg tcgcccagcg ggccgtcaga acctggtacg caaggaacgc
    17341 ccgctttttt catcgccgcg attgccgaca ctttgtcacc catcaggcga atagtgtctg
    17401 ctttcgggcc aatgaagata aagccggagc gttcaacctg ctcggcaaag ttggcgttct
    17461 cggagaggaa gccgtaaccc ggatggattg ctactgcgcc ggtgatttca gcggcgctta
    17521 tgattgccgg gatgttcaga taacttttta ctgacggagc agggccaata cagaccgttt
    17581 catctgccag taatacgtgt tttagatcgc gatccgcgct ggagtgcaca gcgacagtct
    17641 tgatgcccag ttctttacag gcacgaagaa tacgcaatgc aatctcgccg cggttggcaa
    17701 taacaatttt atccagcatg ttcgcctcgt tactcgatga cgaccagcgg ctcgtcaaat
    17761 tctaccggtt gtccactttc gaccagaatt gctttcacgg taccggattt gtccgcttcg
    17821 atctggttca tcattttcat ggcttcaacg atgcacaggg tatcgcccac gttgactttc
    17881 tgacccactt cgatgaacgc ttttgcgtcc gggcttgggg tgcggtagaa agtaccaacc
    17941 atcggggagc gtacgatgtg accactgatt tccgctgctg ctggcgcttc catggaagga
    18001 acggtcgccg gagcggctgc gttagattga gctggctgct gcatcattgg tgcagcgtaa
    18061 gcttgttgca tcacagggaa acttgcggca ggagctgcac ggctgatgcg tactgactct
    18121 tcgccttcgg agatttccag ttcggagatg cctgattctt caaccagctc gatcagtttt
    18181 ttaatcttac gaatatccat gagtgggttc cgtactcttt gtttagtgtg attgtgacag
    18241 gcgtttcacc gccgtctgta aagcgtatgc cccgacagct gtatgcatag cgataaattc
    18301 cagcaggccg ggacgccggt ctattttgcc ccagagtgtc aatgttagac ttgacggaca
    18361 ttgtgcagcg atctgctgct atcctccggc aaaaaacaaa atataccttc gttgcgccaa
    18421 tgtcaccttt tccgcagcgc aaaaactgcg tcagggagga cgaggtcgca cattataacg
    18481 atttcgtagc aattggcagc taaatactgg tcttatcagg gaagataatc aacagctaac
    18541 atgtaaataa ccttcaacac cgtgtaattt gcaacaagcc gcacaattca cgaaattagc
    18601 gccatcatcg acggaacttc ttataacgta aggtgaaaag ctgaaaagcc agccctgcga
    18661 agatgagggg ctgcaatgag ataatcttca cagaccacaa ataatgtagg gatgccagga
    18721 tcgcgacaag atagacgaca ttacgcagcg gttgctgggg attacccaat tttcactgca
    18781 tcgtctggat agatgtgaat gctaaagcac gcaaaatcaa ccgactgaca attctcagca
    18841 ttaaataggt tcgagtggtt aatttgtagt caaataaaat cagattattc accccatggg
    18901 accagaaatc agtaacaggt taagcgtagt gtcgcccagg caaagcacca taaacctaac
    18961 aggcgacgga ggcatatcaa taatggctgt ttagaggagt atcaatgcgc atcaatttgt
    19021 ggtcagtcga aatcatgact ttccagtttg gatgatgttg tacaacttcc tgaccaaggt
    19081 gttcgacgaa agctgtccgg ctaaccgctt ttcccgttaa ccgattgaat aatgatgtca
    19141 aaatgagttc cctctcttat tattccttgc taaatggtta gttaaccttc accagcgtgc
    19201 gaccctggat ctggttatta atgatggcct cggcaaagtt cggtgcctct gacagagata
    19261 tctcttttgc cgcctgggta tagaatgatt ccggtaaatc ggcgaccagt cgctgccagg
    19321 cttgtgcgcg gcgttctggt ggcgtcatta ctgaatccac cccttgcaaa cggacattac
    19381 gcagaataaa tggcatgacc gtggttggca gagtaaaacc acccgccaga ccacaggccg
    19441 ccacgcagcc gccgtaattc atttgcgcca gcacttttgc cagcactttg tcgccaacag
    19501 tgtcaattgc cccagcccag acttgttttt ccagaggacg ggattcggca aactcatcac
    19561 gagggagaat acgactggca cctaaacttt tcagatattc atgggtactt tcgcgaccgg
    19621 aaacggcaac gacctgataa cccaacttat gcagcagcgc cacggcggta ctgccgacgc
    19681 caccactggc acccgtcacg acaatctccc cgtcctgcgg gcgaacaccg gcatcttcca
    19741 gcgccatcac acacagcatg gcggtaaaac cggcagtacc gataatcatt gctttacgcg
    19801 cgtccagccc ttgcggcatg gcaaccagcc agtcaccttt cactcgcgcc tgctccgcca
    19861 gcccacccca gtggttttca ccaacgcccc agccagtgag taacacctcc tgaccggcat
    19921 gaaaacgcgg atcttcgctg gtgcgtacag ttccggcaaa atcgatccca ggaatcatcg
    19981 gaaaattacg gatgattttt cccttaccgg taatcgccag cgcatcttta tagttcaggc
    20041 tcgaccagtg aacatcaacc gtgacatcgc cctccggcag gcgactttcg tcaagtgtct
    20101 gtaccgatgc gagagttttg ccgtcctgct gttctaaaag taacgcctgc ataagtggtc
    20161 ctcatgtgca tgatggattg gaaaataatt tctgaagact atactcgcta atggaaaagc
    20221 aaagccgatg aagcgcaata aattgcgaga taaatctgat tt
//
LOCUS       DAPHDW010000074        20171 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.074, whole genome shotgun sequence.
ACCESSION   DAPHDW010000074 DAPHDW010000000
VERSION     DAPHDW010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.074"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            117..1094
                     /locus_tag="RH057_002709"
     CDS             117..1094
                     /locus_tag="RH057_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HDV3129058.1"
                     /translation="MIFTSTCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEHAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVVWYQIAAESGMSYAQNNLGWMYRNGNGVAKDYALAFFWYK
                     QAALQGHSDAQNNLADLYEDGKGVAQNETLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQGSVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEDLL"
     gene            complement(1164..1646)
                     /locus_tag="RH057_002710"
     CDS             complement(1164..1646)
                     /locus_tag="RH057_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HDV3129059.1"
                     /translation="MNKVAQYYRELVASLSERLRNGERDIDALVEQARERVIKTGELT
                     RTEVDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EVFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGHDQFQRRPFEP
                     "
     gene            1881..4463
                     /gene="leuS"
                     /locus_tag="RH057_002711"
     CDS             1881..4463
                     /gene="leuS"
                     /locus_tag="RH057_002711"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HDV3129060.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALLAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            4478..5059
                     /gene="lptE"
                     /locus_tag="RH057_002712"
     CDS             4478..5059
                     /gene="lptE"
                     /locus_tag="RH057_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HDV3129061.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAECQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTTLGN"
     gene            5059..6090
                     /gene="holA"
                     /locus_tag="RH057_002713"
     CDS             5059..6090
                     /gene="holA"
                     /locus_tag="RH057_002713"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HDV3129062.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAARAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            6092..6733
                     /gene="nadD"
                     /locus_tag="RH057_002714"
     CDS             6092..6733
                     /gene="nadD"
                     /locus_tag="RH057_002714"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HDV3129063.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            6757..7368
                     /locus_tag="RH057_002715"
     CDS             6757..7368
                     /locus_tag="RH057_002715"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HDV3129064.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLHG
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEFQHYQNILVVSHQGVLSLLIARL
                     IGMPAEAMWHFRVDQGCWSTIDINQKFATLRVLNSRAIGVENA"
     gene            7628..7945
                     /gene="rsfS"
                     /locus_tag="RH057_002716"
                     /gene_synonym="rsfA"
     CDS             7628..7945
                     /gene="rsfS"
                     /locus_tag="RH057_002716"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HDV3129065.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            7949..8416
                     /gene="rlmH"
                     /locus_tag="RH057_002717"
     CDS             7949..8416
                     /gene="rlmH"
                     /locus_tag="RH057_002717"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HDV3129066.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSTLTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            8447..10348
                     /gene="mrdA"
                     /locus_tag="RH057_002718"
     CDS             8447..10348
                     /gene="mrdA"
                     /locus_tag="RH057_002718"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HDV3129067.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     DPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTAHKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            10351..11463
                     /gene="mrdB"
                     /locus_tag="RH057_002719"
                     /gene_synonym="rodA"
     CDS             10351..11463
                     /gene="mrdB"
                     /locus_tag="RH057_002719"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HDV3129068.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            11474..12562
                     /gene="rlpA"
                     /locus_tag="RH057_002720"
     CDS             11474..12562
                     /gene="rlpA"
                     /locus_tag="RH057_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HDV3129069.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTQLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTAPSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            12702..13913
                     /gene="dacA"
                     /locus_tag="RH057_002721"
     CDS             12702..13913
                     /gene="dacA"
                     /locus_tag="RH057_002721"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HDV3129070.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFGDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            14024..14287
                     /locus_tag="RH057_002722"
     CDS             14024..14287
                     /locus_tag="RH057_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HDV3129071.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            14388..15029
                     /gene="lipB"
                     /locus_tag="RH057_002723"
     CDS             14388..15029
                     /gene="lipB"
                     /locus_tag="RH057_002723"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HDV3129072.1"
                     /translation="MYQDKILVRQLGLQPYEPISQAMHEFTDTRDDSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            15288..16241
                     /locus_tag="RH057_002724"
     CDS             15288..16241
                     /locus_tag="RH057_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HDV3129073.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYITQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICREGNPLLSLEDDRETIDNAAHVLLL
                     PEEQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFSLRDPILHGVIDVIRNAF"
     gene            16450..17415
                     /gene="lipA"
                     /locus_tag="RH057_002725"
     CDS             16450..17415
                     /gene="lipA"
                     /locus_tag="RH057_002725"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HDV3129074.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(17516..17719)
                     /gene="tatE"
                     /locus_tag="RH057_002726"
     CDS             complement(17516..17719)
                     /gene="tatE"
                     /locus_tag="RH057_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HDV3129075.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(17848..18636)
                     /locus_tag="RH057_002727"
     CDS             complement(17848..18636)
                     /locus_tag="RH057_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HDV3129076.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHVPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRRVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPVLNNRRFAPPQLL"
     gene            18729..19112
                     /gene="crcB"
                     /locus_tag="RH057_002728"
     CDS             18729..19112
                     /gene="crcB"
                     /locus_tag="RH057_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HDV3129077.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTAH"
     gene            complement(19166..19375)
                     /gene="cspE"
                     /locus_tag="RH057_002729"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(19166..19375)
                     /gene="cspE"
                     /locus_tag="RH057_002729"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV3129078.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(19550..20110)
                     /gene="pagP"
                     /locus_tag="RH057_002730"
     CDS             complement(19550..20110)
                     /gene="pagP"
                     /locus_tag="RH057_002730"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HDV3129079.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADEWMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
BASE COUNT         5084 a         5092 c         5077 g         4918 t
ORIGIN      
        1 aggctcgttt atttatactt ttctgacgcg acataatacg cataaaataa ggcttacgca
       61 aaaatggaac acataaaaat ataatatgtt ttatctgcaa ccataaggaa atgataatga
      121 ttttcacgtc aacttgctgc gataatttat caatagatga aattatcgaa cgtgctgaaa
      181 aaggcgattg cgaggctcaa tatattgttg ggttttatta taatcgcgat agcgcaattg
      241 attctccgga cgacgaaaaa gccttttact ggctgaagct ggccgccgag caaggtcatt
      301 gtgaagcaca atattcctta gggcaaaagt atactgagga taaaagccgt cataaagata
      361 atgagcatgc catcttttgg ctgaaaaaag ctgccctaca aggccatact ttcgcttcca
      421 acgcccttgg ctggatactg gatcgtggag aagaccccaa ttataaagaa gcggttgtct
      481 ggtatcagat agccgcggag agcggaatgt cttatgcgca aaataatctt gggtggatgt
      541 acagaaatgg caacggagtc gcaaaagact atgcgctggc atttttctgg tacaaacaag
      601 ctgcattaca aggccatagt gacgcgcaaa acaatctggc cgatctttat gaagacggaa
      661 aaggcgttgc tcaaaacgag acactcgccg cattctggta tttgaaaagc gcacagcagg
      721 gtaatcggca tgctcagttt caaattgcat gggattataa cgctggcgaa ggggtggacc
      781 aggactataa gcaagcgatg tactggtatc tgaaggctgc cgctcagggg agcgtcggcg
      841 cttacgtcaa catcggttat atgtataaac acggacaagg cgttgagaaa gattatcagg
      901 ctgcctttga atggtttacg aaagccgctg aatgcaatga cgccactgcc tggtataacc
      961 tggccattat gtatcactac ggagaaggaa gacctgtcga tctccgacag gctctcgacc
     1021 tgtatcgtaa agttcagtca tccggaacca gagatgtcag tcaggaaatt cgtgagactg
     1081 aagatttact gtagagataa aaacaagggc cggggaattt cattcggccc atattctctt
     1141 tattgctgtg cgaaatcaga ctattacggc tcaaacgggc gtctctggaa ctggtcatga
     1201 ccacatttcg ggcatagcgt cagcacttcc ggtgtgtaga tcgggagatg gaagtgacat
     1261 ttctcgcaga ccagatttcc cagcccgacc acttctccgc tgtgataaac cccatgatga
     1321 ttgaggtcct ggaaaacttc gcgccattca agctgcgttt tatcggtgat gtctgccagc
     1381 tcctgccaca agctttcttt aatcacccgc ataaagacgc tgtcagattc ttctttcagg
     1441 ctctcttcat agctcatggc gaactcttcc aggtcacgtc tgacagctcg cgtcagctca
     1501 tcgacctcgg ttcgcgttaa ctcccctgtt tttattacgc gctcgcgcgc ctgttccacc
     1561 agtgcgtcga tatcacgttc gccattgcgc aggcgttcgc tcagtgacgc aaccagttca
     1621 cggtaatatt gagcaacctt gttcatcgtt tcatctcccg ggtcgttaac tgactataat
     1681 aatagacgtt ctttgttcct tgctttgcta atacgcccac aaactctgta aattaatgta
     1741 tgaataatcc gggcatcatt tcaacgtcga gtgcgcgaaa ggctgttttg acgcggacgt
     1801 ttgggctatg ctatgcggat ctgaaaaacc acctcaacgc tacatttgta gccgtattga
     1861 aaacaggacc actggctgcc atgcaagagc aataccgccc ggaagagata gaatccaaag
     1921 tacagcttca ttgggatgag aagcgcacat ttgaagtaac cgaagacgag agcaaagaga
     1981 agtattactg cctgtctatg cttccctatc cttctggtcg actacacatg ggccacgtac
     2041 gtaactacac catcggtgac gtgatcgccc gctaccagcg tatgctgggc aaaaacgtcc
     2101 tgcagccgat cggctgggac gcgtttggtc tgcctgcgga aggcgcggcg gtgaaaaaca
     2161 ataccgctcc ggcaccgtgg acgtacgaca acatcgcgta tatgaaaaac cagctcaaaa
     2221 tgctgggctt tggttatgac tggagccgcg agctggcaac ctgtacgccg gaatactacc
     2281 gttgggaaca gaaattcttc accgagctgt ataaaaaagg cctggtatat aagaagactt
     2341 ctgcggtcaa ctggtgcccg aacgaccaga ccgtactggc gaacgaacaa gttatcgacg
     2401 gctgctgctg gcgctgcgat accaaagttg aacgtaaaga gatcccgcag tggtttatca
     2461 aaatcactgc ttacgctgac gagctgctca acgatctgga taaactggat cactggccag
     2521 acaccgttaa aaccatgcag cgtaactgga tcggtcgttc cgaaggcgtg gagatcacct
     2581 tcaacgttaa cgactatgac aacacgctga ccgtttacac tacccgcccg gacaccttta
     2641 tgggttgtac ctacctggcg gtagctgcgg gtcatccgct ggcgcagaaa gcggcggaaa
     2701 ataatcctga actggcggcc tttattgacg aatgccgtaa caccaaagtt gccgaagctg
     2761 aaatggcgac gatggagaaa aaaggcgtcg atactggctt taaagctgtt cacccattaa
     2821 cgggcgaaga aattcccgtt tgggcagcaa acttcgtatt gatggagtac ggcacgggcg
     2881 cagttatggc ggttccgggt cacgaccagc gcgactacga gtttgcctct aaatacggcc
     2941 tgaacatcaa accagttatc ctggctgctg acggctctga gccagatctt tctcagcaag
     3001 ccctgactga aaaaggcgtg ctgttcaact ctggcgaatt caacggtctt gaccatgaag
     3061 cggccttcaa cgccatcgcc gataaactga ctgcgatggg cgttggcgag cgtaaagtga
     3121 actaccgcct gcgcgactgg ggtgtttctc gtcagcgtta ctggggcgcg ccgattccga
     3181 tggtgacgct ggaagacggt accgtaatgc cgaccccgga cgaccagctg ccggtgatcc
     3241 tgccggaaga tgtggtgatg gacggcatta ccagcccgat taaagcagat ccggagtggg
     3301 caaaaactac cgttaacggt atgccagcgc tgcgtgaaac cgacactttc gacaccttta
     3361 tggagtcctc ctggtactat gcgcgctaca cttgcccaga gtacaaagaa ggtatgctgg
     3421 attccgaagc ggctaactac tggctgccgg tggatatcta cattggtggt atcgaacacg
     3481 ccattatgca cctgctctac ttccgcttct tccacaaact gatgcgtgat gcaggcatgg
     3541 tgaactctga cgaaccagcg aaacagttgc tgtgtcaggg tatggtgctg gcagatgcct
     3601 tctactatgt tggcgaaaac ggcgaacgta actgggtttc cccggttgat gctatcgttg
     3661 aacgtgacga gaaaggccgt atcgtgaaag cgaaagatgc ggcaggccat gaactggttt
     3721 ataccggcat gagcaaaatg tccaagtcga agaacaacgg tatcgacccg caggtgatgg
     3781 ttgaacgtta cggcgcggac accgttcgtc tgtttatgat gtttgcttct ccggctgata
     3841 tgactctcga atggcaggaa tccggtgtgg aaggggctaa ccgcttcctg aaacgtgtct
     3901 ggaaactggt ttacgagcac acagcaaaag gtgatgttgc ggcactgaac gttgatgcgc
     3961 tgactgaaga tcagaaagcg ctgcgtcgcg atgtgcataa aacgatcgct aaagtgaccg
     4021 atgatatcgg ccgtcgtcag accttcaaca ccgcaattgc ggcgattatg gagctgatga
     4081 acaaactggc gaaagcacca accgatggcg agcaggatcg cgctctgatg caggaagcgc
     4141 tgctggccgt tgtccgtatg cttaacccgt tcaccccgca catctgcttc acgctgtggc
     4201 aggaactgaa aggcgaaggc gatatcgaca acgcgccgtg gccggttgct gacgaaaaag
     4261 cgatggtgga agactccacg ctggtcgtgg tgcaggttaa cggtaaagtc cgtgccaaaa
     4321 tcaccgttcc ggtggacgca acggaagaac aggttcgcga acgtgctggc caggaacatc
     4381 tggtagcaaa atatcttgat ggcgttaccg tgcgtaaagt gatttacgta ccaggtaaac
     4441 tcctcaatct ggtcgttggc taagcgcggg aggaagcgtg cgatatctgg caacattgtt
     4501 gttatctctg gcggtgttaa tcaccgccgg gtgtggctgg catctgcgtg ataccacgca
     4561 ggttccttcc actatgaagg tcatgatcct ggactcaggc gatccgaacg ggccattaag
     4621 ccgtgcggtg cgtaaccagt tacgtctgaa tggtgtcgag ttgcttgata aagaaaccac
     4681 gcgtaaggac gttccatccc tgcgtttggg taaagtgagc atcgcgaaag ataccgcatc
     4741 ggtattccgt aacggtcaaa cagcagagtg tcagatgatc atgacggtga atgcgaccgt
     4801 gttgatcccc ggccgtgata tctacccgat tagcgccaaa gtcttccgtt cgttctttga
     4861 taacccgcaa atggcgttag cgaaagataa cgaacaagac atgatcgtaa aagagatgta
     4921 cgaccgtgct gccgaacagc tgattcgtaa gctgccaagc atccgtgctg cggatattcg
     4981 ttccgacgaa gaacagacgt cgaccacaac ggatactccg gcaacgcctg cacgcgtctc
     5041 caccacgctg ggtaactgat gattcggttg tacccggaac aactccgcgc gcagctcaat
     5101 gaagggctgc gcgcggcgta tcttttactt ggtaacgatc ctctgttatt gcaggaaagc
     5161 caggacgctg ttcgtcaggt agctgcggca caaggattcg aagaacacca cactttttcc
     5221 attgatccca acactgactg gaatgcgatc ttttcgttat gccaggctat gagtctgttt
     5281 gccagtcgac aaacgctatt gctgttgtta ccagaaaacg gaccgaatgc ggcgatcaat
     5341 gaacaacttc tcacactcac cggacttctg catgacgacc tgctgttgat cgtccgcggt
     5401 aataaattaa gcaaagcgca agaaaatgcc gcctggttta ctgcgcttgc gaatcgcagc
     5461 gtgcaggtga cctgtcagac accggagcag gctcagcttc cccgctgggt tgctgcgcgc
     5521 gcaaaacagc tcaacttaga actggatgac gctgcaaatc aggtgctctg ctactgttat
     5581 gaaggtaacc tgctggcgct ggctcaggca ctggagcgtt tatcgctgct ctggccagac
     5641 ggcaaattga cattaccgcg cgttgaacag gcggtgaatg atgccgcgca tttcacccct
     5701 tttcattggg ttgatgcttt gttgatggga aaaagtaagc gcgcgttgca tattcttcag
     5761 caactgcgtc tggaaggcag cgagccggtt attttgttgc gcacattaca acgtgaactg
     5821 ttgttactgg ttaacctgaa acgccagtct gcccatacgc cactgcgtgc gttgtttgat
     5881 aagcatcggg tatggcagaa ccgccggggc atgatgggcg aggcgttaaa tcgcttaagc
     5941 cagccgcagt tacgtcaggc cgtgcaactc ctgacacgaa cggaactcac cctcaaacaa
     6001 gattacggtc agtcagtgtg ggcagagctg gaagggttat ctcttctgtt gtgccataaa
     6061 cccctggcgg acgtatttat tgacggttga tatgaaatct ttacaggctc tgtttggcgg
     6121 cacctttgat ccggtgcact atggtcatct taaacccgtg gaaacgctgg cgaatttgat
     6181 tggtctgacg cgggtcacaa tcatccctaa taatgttcct ccgcatcgtc cccagccgga
     6241 agcgaacagc gtgcagcgta aacacatgct tgaactggcg attgccgaca agccattatt
     6301 tactcttgat gaacgcgagc taaagcgcaa tgccccctct tacactgcgc aaacactgaa
     6361 agagtggcgg caggaacaag ggccggacgt gccgctggcg tttattattg gtcaggattc
     6421 attgctgacc tttccgacct ggtacgaata cgaaacgata ctcgacaatg cacatttgat
     6481 cgtctgtcgg cgtccaggtt acccacttga aatggcgcaa ccgcaatacc agcaatggct
     6541 ggaagatcat ttgacacata acccggaaga tcttcacctt cagcctgccg gtaaaattta
     6601 tctggctgaa acgccgtggt ttaacatctc ggcgaccatc atccgcgaac gtttgcaaaa
     6661 cggtgaatcg tgtgaggatt tattgccgga accggtactg acttacatta accaacaagg
     6721 cttgtatcgc tgatacctgt cgttacggag ggcatgatgc gactgtggtt aattcgtcat
     6781 ggcgaaacgc aagcgaatgt cgatggtctg tacagcggtc atgcgcccac acccctgacc
     6841 gcacgcggta ttgagcaagc gcaaaatctg catacgctgc tacatggtgt ttcctttgat
     6901 ctggttttat gcagtgaact ggaacgggca cagcataccg cgcgactggt tctcagtgac
     6961 cgccagctcc ccgttcatat catccctgaa ctcaacgaaa tgttttttgg cgactgggag
     7021 atgcgacatc atcgcgacct catgcaagaa gatgccgaaa actatagcgc gtggtgcaat
     7081 gactggcagc atgcaatccc cacgaacggt gaaggatttc aggcattttc gcaacgtgtg
     7141 gaacgcttta tcgcaaggct tagtgaattt cagcactatc agaatatttt agtcgtcagc
     7201 catcagggtg tactgagtct gttaatcgcc cgtttaattg gcatgcctgc cgaggctatg
     7261 tggcattttc gcgttgacca ggggtgttgg agcaccattg atatcaacca aaaattcgcg
     7321 acgctacgcg tcctcaatag ccgtgccatc ggggtcgaga atgcatgact tttctgtttt
     7381 tttacgggta agccgcaacg accattgaca gacccgggca ggctgatatt ctccgcagcc
     7441 agactttttt cgccagacac gactttgtag aaattgtttt acaaaaatgg cgatgcaatc
     7501 tgcggcgcgg ggtgggatga tagcccactt tcgaaagccg attcggcgac aattgtcccg
     7561 aaatcgcctc tggttcaggt atactgacag accattttta tctatttgat tcacccaggg
     7621 ggaaaacttg cagggtaaag cactccagga ttttgttatc gacaaaattg atgacctcaa
     7681 aggtcaggac atcatcgcct tagacgttca aggcaaatcc agcatcaccg actgcatgat
     7741 catctgtacg ggtacgtcca gccgtcatgt tatgtccatt gctgaccacg ttgtgcagga
     7801 gtctcgcgca gcgggcctgt taccgctcgg cgtagaaggt gaaaacagcg ccgactggat
     7861 tgtcgtggat ttgggcgatg tgattgtcca tgtcatgcag gaagagagcc gtcgcctgta
     7921 tgaactggaa aaactctgga gttaatgcgt gaagctgcaa cttgtcgccg tgggaacgaa
     7981 aatgccggac tgggtacaaa ccggttttac cgagtacctg cgtcgttttc cgaaagatat
     8041 gcccttcgag ctgattgaaa ttccggccgg aaaacgcggc aagaatgcgg acatcaagcg
     8101 catactcgac aaagagggtg agcagatgtt ggcggccgca ggcaaaaacc gcattgtcac
     8161 cctcgatatt ccaggcaagc cctgggatac gccgcagtta gccgctgagc tggaacgctg
     8221 gaagctggat ggtcgcgacg tcagtctact gattggcggg cctgaagggt tgtcgcctgc
     8281 ctgtaaagcg gcggcggagc agagctggtc gctgtcgacg cttaccctcc cccatccgct
     8341 ggttcgcgtg ctggtcgcag agagtctgta ccgggcgtgg agcatcacca ccaaccatcc
     8401 ttatcaccgt gagtgataag ggagctttga gtagaaaacg cagcggatga aactacagaa
     8461 ctcttttcgc gactatacgg cagagtccgc gctgtttgtg cgccgggcgc tggtcgcctt
     8521 tttggggatt ttgctgctga ccggcgtgct tatcgccaac ctgtataatc tgcaaattgt
     8581 tcgctttacc gactaccaga ctcgctctaa tgaaaaccgc attaagctgg tgcctatcgc
     8641 gcccagccgc ggcattatct acgaccgtaa tggtatccct ctggccctca accgcactat
     8701 ctaccagata gaaatgatgc cagagaaagt cgataacgtg cagcaaacgc tggacgcttt
     8761 gcgcagcgtg gttgatctga ccgatgacga tattgctgca ttccgaaaag agcgcgcacg
     8821 ttcacaccgt ttcacctcta ttccggtgaa aactaacctg accgaagtac aagtagctcg
     8881 ctttgccgtc aatcagtacc gttttccggg tgtcgaagtt aaaggctata aacgtcgtta
     8941 ctatccttac ggttcggcgt tgacccacgt catcggctat gtgtcgaaaa tcaacgataa
     9001 agacgtcgaa cgcctgaata atgacggcaa actggccaac tatgcggcaa cgcatgatat
     9061 cggtaagctg ggcattgagc gttactatga agacgtgctg cacggtcaga ccggttatga
     9121 agaggttgaa gttaacaacc gtgggcgtgt tatccgccag ttaaaagaag taccaccgca
     9181 agccggacac gatatttacc tgacgctgga tctcaaactc cagcaatata ttgaaacgct
     9241 gctggcgggt agccgcgcag ctgtggttgt caccgatccg cgtacaggtg gggtgctggc
     9301 gctggtttcc acgcctagtt atgacccaaa cctgtttgtt gacggtatct ccagcaaaga
     9361 ttattccgcc ttgttgaacg acccgaatac accgctggtg aaccgcgcca cacagggggt
     9421 ttatcctccc gcgtctacag ttaaacccta tgtggcggtt tcggcattga gcgccggggt
     9481 gatcacgcgc aatacgacgc tgtttgaccc aggctggtgg caactgccag gttcggaaaa
     9541 acgttatcgt gactggaaaa aatggggcca cgggcgtctg aatgtcacaa gatcgctgga
     9601 agaatctgcg gataccttct tctatcaggt ggcctacgat atggggatcg atcgcctctc
     9661 cgaatggatg ggtaaattcg gttatggtca ttacaccggt atcgacctgg cggaagaacg
     9721 ttccggcaac atgcctaccc gcgaatggaa acagaaacgc tttaaaaaac cgtggtatca
     9781 gggtgacacc attccggttg gtatcggtca gggttactgg acagcgaccc caatccagat
     9841 gagtaaggca ctgatgatcc tgattaatga cggtatcgtg aaggttcctc atttgctgat
     9901 gagcaccgcc gaagacggca aacaggtgcc atgggtacag ccgcatgaac cgcccgtcgg
     9961 cgatattcat tccggttact gggagctggc gaaagacggt atgtacggtg ttgctaaccg
    10021 ccctaacggt acggcgcata aatactttgc cagcgcaccg tacaaaattg cggcgaaatc
    10081 cggtaccgct caggtcttcg gtctgaaagc gaacgaaacc tataatgcgc acaaaattgc
    10141 cgagcgttta cgtgaccaca aactgatgac cgcctttgcg ccatacaaca atccgcaagt
    10201 ggctgtcgcc atgattctgg agaacggtgg tgcgggtccg gcggttggga cactgatgcg
    10261 ccagatcctc gaccacatta tgctgggtga taacaacacc gatctgcctg cggaaaatcc
    10321 agcggttgcc gcagcggagg accattaatc atgacggata atccgaataa aaaaacattc
    10381 tgggataaag tccatctcga tcccacaatg ctgctgatct tactggcatt gctggtttac
    10441 agcgccctgg ttatctggag cgccagcggt caggatattg gcatgatgga gcgtaaaatc
    10501 ggccaaatcg cgatgggtct ggtcatcatg gtggtgatgg cgcaaattcc tccacgcgtt
    10561 tatgaaggct gggcccccta tctctatatc atctgtatta ttttgctggt ggcggtagat
    10621 gctttcggtg ccatctctaa aggtgctcaa cgctggctgg acctcggtat tgttcgtttt
    10681 cagccgtcgg aaattgccaa aatagccgta ccactgatgg ttgcgcgctt tatcaaccgc
    10741 gacgtttgcc cgccatcgtt gaagaacact ggcatcgcgc tggtgctgat atttatgccc
    10801 acgctgctgg tggctgcaca gcctgacctg ggaacatcaa tcctcgttgc gctttccggt
    10861 ctgtttgtac tgttcctctc tggccttagc tggcgtctga ttggcgtcgc agtagtgctg
    10921 gtggcggcgt tcattccgat tctgtggttc ttcctgatgc atgattacca gcgccagcgc
    10981 gtaatgatgc tcctggaccc ggaatcagac ccactcggcg cgggctatca cattattcag
    11041 tctaaaattg ctattggctc cggcggatta cgcggcaaag gctggctgca cggcactcag
    11101 tcacagcttg aatttctccc cgaacgccat accgacttta tcttcgcggt actggcggaa
    11161 gagctgggat tagtgggcat tttgattctg ctcgctctct acattctgct gatcatgcgc
    11221 gggctgtgga tagccgccag agcgcaaacc acctttggtc gcgtcatggc tggtggctta
    11281 atgctgatat tattcgttta tgtcttcgta aatattggta tggtaagcgg tattctgccg
    11341 gttgtagggg ttccgctccc actggtcagt tatggaggat cggcgctaat tgtgctgatg
    11401 gctgggttcg ggattgtaat gtcaatccac acccacagga aaatgttgtc gaaaagcgtg
    11461 taagaggtgc gcaatgcgta agcagtggct cgggatctgc atcgcggcag gaatgctcgc
    11521 ggcatgtaca agcgatgatg gtcagcaaca gacggtaagt gtaccgcagc ctgcggtatg
    11581 taacggcccc atagttgaaa ttagcggggc ggacccgcgt ttcgaaccac tgaacgcgac
    11641 ggcaaatcag gattaccagc gcgacggtaa aagctacaaa atcgtgcagg atccgtctcg
    11701 atttagccag gcgggactgg cggcaatcta tgatgccgaa ccaggcagta acctgacggc
    11761 ctctggcgaa gctttcgatc cgacacagct gacggcggcc catccaacgc ttccgatccc
    11821 cagctacgcc agaatcacta acctggctaa cgggcgaatg atcgtggtgc gcattaatga
    11881 tcgcggccct tacggcaacg accgcgttat ttcgctttct cgcgcggcag ctgaccgtct
    11941 taacacgtca aacaacacca aagttcgtat cgatccgatt attgtcgccc aggatggttc
    12001 gctttctggt cctggtatgg cttgtaccac agtcgccaaa cagacttacg ccctgcctgc
    12061 acctcccgat ttaagcggtg gcgcgggaac aagttcagtg tctggcccgc agggtgacat
    12121 tcttccggtc agtaattcga cgctaaaaag cgaagatccg accggcgcgc cggtaaccag
    12181 tagcggtttc ctcggcgcac caacgacctt agcgcctggt gtactggaag gcagcgaacc
    12241 gacgcctgcg ccacagcccg ttgttacagc tccgtcgaca acgcctgcaa cctcgcctgc
    12301 aatggtgaca ccgcaagccg cctcgcaaag cgccagcggc aactttatgg tgcaagtcgg
    12361 ggccgttagc gatcaggctc gtgcgcaaca gtaccaacag caactgggac agaagttcgg
    12421 cgtccccggt cgcgtaactc aaaatggcgc ggtctggcgg atccagcttg gccccttcgc
    12481 cagcaaagcc gaagccagta ccttgcagca acgtttgcaa accgaagccc aattacagtc
    12541 atttattacc accgcgcagt agcgtaaagc aggcatctga tgtgtcaatt tctgtaaatg
    12601 acgttaacaa agtcacgcgg aaagtcagat gcctgccggt agtgcatttg ctatagtagg
    12661 gcactttttt taattccatc acggatgtcg tagttcagac catgaatacc attttttccg
    12721 ctcgtatcat gaagcgcctg gcgctcacca cggctctttg cacagccttt atctctgctg
    12781 cacatgccga tgacctgaat atcaaaacta tgatcccggg tgtaccgcag atcgatgcgg
    12841 agtcctacat cctgattgac tataactccg gcaaagtgct cgctgaacag aacgcagatg
    12901 tccgccgcga tcctgccagc ctgaccaaaa tgatgaccag ttacgttatc ggccaggcaa
    12961 tgaaagccgg taaatttaaa gaaactgatt tagtcactat cggcaacgac gcatgggcca
    13021 ccggtaaccc ggtgtttaaa ggttcttcgc tgatgttcct caaaccgggc atgcaggttc
    13081 cggtttctca gctgatccgc ggtattaacc tgcaatcggg taacgatgct tgtgtcgcca
    13141 tggccgattt tgccgctggt agccaggacg cttttgttgg cttgatgaac agctacgtta
    13201 acgcactggg cctgaaaaat acccacttcc agacggtaca tggtctggat gctgatggtc
    13261 agtacagctc cgcgcgagat atggcgctga tcggccaggc attgatccgt gacgtaccga
    13321 atgaatactc gatctataaa gaaaaagaat ttacgtttaa cggtattcgc cagctgaacc
    13381 gtaacggcct gttatgggat aacagcctga atgtcgacgg catcaaaacc ggacacactg
    13441 acaaagcagg ttacaacctt gttgcttctg cgactgaagg ccagatgcgc ttgatctctg
    13501 cggtgatggg cggacgtact tttaaaggcc gtgaagccga aagtaaaaaa ctgctaacct
    13561 ggggcttccg tttcttcgaa accgttaacc cactgaaagt aggtaaagag ttcgcctctg
    13621 aaccggtttg gtttggtgat tctgatcgcg cttcgttagg tgttgataaa gacgtgtacc
    13681 tgaccattcc gcgtggtcgc atgaaagatc tgaaagccag ctatgtgctg aacagcagtg
    13741 aattgcatgc gccgctgcaa aagaatcagg tcgtcggaac tatcaacttc cagcttgatg
    13801 gcaaaacgat cgaacaacgc ccgctggttg tgttgcaaga aatcccggaa ggtaacttct
    13861 tcggcaaaat cattgattac attaaattaa tgttccatca ctggtttggt taaaaattga
    13921 acacttgaaa gtgtaatttc cgtccccata tactaagcat cagtaaaaaa actcccgcct
    13981 tctggcggga gttgctattt aattacgtta cgccggagct gacatgaaaa ccaaacttaa
    14041 cgaactgctt gaattcccta ctccttttac ttacaaagtt atggggcagg cgttacctga
    14101 gctggttgat caggtggttg aagtggtaca gcgccatgcg ccaggtgact acaccccaac
    14161 ggtaaaacca agcagcaaag gcaactacca ctcggtatct atcactatca acgccactca
    14221 tatcgagcag gttgaaacac tgtatgaaga actgggcaaa atcgatattg tccgcatggt
    14281 tctgtaactc gcttctccgt tacccggctc ctggtcgggt aactcccctc tcctcgctgt
    14341 gatatacttt tcccccactt ttactcattc tccacggaga tgccgttttg tatcaggata
    14401 aaattcttgt ccgccagctc ggtcttcagc cttacgagcc aatctcccag gctatgcatg
    14461 aattcaccga tacccgcgat gatagtaccc ttgatgaaat ctggctggtc gagcactatc
    14521 cggtattcac ccaaggtcag gcaggaaaag cggagcacat tttaatgccg ggtgatattc
    14581 cggtgatcca gagcgatcgc ggtgggcagg tgacttatca cgggccgggg caacaggtga
    14641 tgtatgtgtt gcttaacctg aaacgccgta aactcggtgt gcgtgaactg gtgaccttgc
    14701 ttgagcaaac agtggtgaat accctggctg aactgggtat agaagcgcat cctcgggctg
    14761 acgcgccagg tgtctatgtt ggggaaaaga aaatttgctc actgggttta cgtattcgac
    14821 gcggttgttc attccacggt ctggcattaa acgtcaatat ggatctttca ccatttttac
    14881 gtattaatcc ttgtgggtat gccggaatgg aaatggctaa aatatcacaa tggaaacccg
    14941 aagcgacgac taataatatt gctccacgtt tactggaaaa tattttagcg ctactaaaca
    15001 atccggactt cgaatatatt accgcttaat tccatacatc aatggcccaa tttacactgg
    15061 gtcattactc ataatacctg ctaatacttt acatctcctt cgcgtttgta tattttcttt
    15121 atcacttgca gaaatgcggt gccacgcctt gctccgtaac tcaccccatt gattaaccta
    15181 ttgaaatagt tgattatggt gagcaaaagc gttccgttcc aattagacat aagattattg
    15241 taatttttta aatattattt ttccatgaat aaaattggag taagtgcgtg gatagtaata
    15301 atcaaattga accctgttta agcagaaagt cgtcagaagg taaaccacaa atatttacaa
    15361 ccttacgaaa tattgatctt aaccttctga ctatttttga agctgtatat gtacataaag
    15421 ggatcgttaa tgcagcgaaa gtgcttaatc tgaccccctc ggcaatcagt cagtctattc
    15481 agaaactgcg cgttatattc cctgacccat tgtttattcg caaaggccag ggtgtcactc
    15541 ctaccgcatt tgcgatgcat ctacatgagt atatcactca gggccttgag tccattcttg
    15601 gcgcgctgga tatcgaagga agctatgata agcaacgaac gataactatt gcgaccactc
    15661 cctcggtcgg agcactggtc cttcctgtca tctatcgggc gattaaaact cactatccgc
    15721 agcttttact gcgcaacccg cccatcagcg acgcggaaaa ccaactcagt cagtttcaaa
    15781 ccgatctcat catcgataac atgttttgca ccaatcgtac ggtgcaacat catgttctgt
    15841 tcaccgacaa tatggtgtta atttgccgcg agggaaatcc actactctct ttagaagatg
    15901 acagagagac tatcgacaac gctgcgcatg tacttctgtt accggaagag caaaatttca
    15961 gcggtctgcg gcagagagtt caagaaatgt ttccggaccg gcaaatcaat ttcaccagct
    16021 acaacatttt gacaatcgct gcactggttg ccaacagtga catgttagcg attattccca
    16081 gccgttttta taacctgttt agccgctgct ggccgctgga aaaactgcct tttccgtcct
    16141 taaatgagga gcaaatagat ttctctatcc actacaacaa attcagcctg cgtgatccca
    16201 tcctgcacgg agtaatagat gttatccgta atgcattttg aaaaagtatt gttacagcca
    16261 gatcacagcg cagaacgata aaatggcaca atccaacaac aattttacat tttcgcgacc
    16321 gctttggctg ctttcaggtc cgtttcaatg atatactgcc agtcgttaat tcaaaaatag
    16381 ttgataatta caacaatcta ttgaattgaa acgctttcct tcgtaattcg caactggaac
    16441 acgcacgcta tgagtaaacc cattgtgatg gaacgcggtg ttaaataccg cgatgccgat
    16501 aagatggccc ttatcccggt taaaaacgtg gcaacagagc gcgaagccct gctgcgcaag
    16561 ccggaatgga tgaaaatcaa gcttccagcg gactctacac gtatccaggg catcaaagcc
    16621 gcaatgcgca aaaatggcct gcattctgtc tgcgaggaag cctcctgccc taacctggcg
    16681 gaatgcttca accacggcac agcaacgttt atgatcctcg gcgctatttg tacccgccgt
    16741 tgtccgttct gtgatgttgc ccacggtcgc ccggtagctc ctgatgccaa tgaaccagtg
    16801 aaactggcgc agaccattgc cgatatggcg ctgcgttatg tggttatcac ctccgttgac
    16861 cgtgatgacc tgcgcgatgg cggtgcccag cactttgcgg attgcattac tgccattcgg
    16921 gaaaaaagcc cgcaaatcaa aattgaaact ctggtgccgg atttccgtgg tcgtatggat
    16981 cgtgctctgg atattctgac tgcaacgcca ccagatgtgt tcaaccataa cctggaaaac
    17041 gtaccgcgta tttaccgtca ggtacggcct ggtgcagatt acaactggtc gctgaagctg
    17101 ctggaacgct ttaaagaagc gcatccggaa atcccgacca agtctggtct gatggtggga
    17161 ctgggtgaaa ccaatgaaga aattattgag gtaatgcgcg acctgcgccg tcatggtgtg
    17221 acgatgttaa cgctggggca atatttgcag ccaagccgcc atcacctgcc ggttcaacgt
    17281 tacgttagcc cggatgagtt cgacgaaatg aaagccgaag cgctggcgat gggctttacc
    17341 catgctgcat gcggtccgtt tgtccgctct tcttaccacg ccgatttgca ggcgaaaggg
    17401 atggaagtta agtaatcctg taataattac ttacatctgt ctgataaaaa acccgctttt
    17461 tgaagcgggt tttttgtatc gaacaagata ttgagggagc gtcctgctcg ccacgtcact
    17521 ctttatgaga gagcttttca gcctgaagat caacgtctgc gccttttttc gccgcagcat
    17581 cgtcatcatt catcgccttc ttgaaccctt taatggccgc tccaaggtct ccgcccagcg
    17641 tacgtaactt cttagtccca aacagcagaa cgaccagcgc cgcaactacc agcagtttgg
    17701 taatactaat ctcacccata gataccttct tgacatataa ccggcaacag aacgcgcctt
    17761 aaaaaccaga gaatattaac ggtcattcga cgcgcgcaca caatagcaat ctgtaacaag
    17821 gtgaatcaag cgccgagtag aaaaacatca taataattgc ggcggcgcaa agcgacggtt
    17881 gtttaagacg ggcagttgcg cgcgcacctg acgcacacgt tcgggcgtca cttccgccat
    17941 aatgagtgca ggcatttctg acgctgccgc aatggtgacg ccaaagggat ctataatccg
    18001 gctttgaccg atatttttgt tcccgcactc ccccgccgcc accatataac aggtggtatc
    18061 cagcgcacga gcggcaagca acgttgacca gtgatgctct ttgagcggcc cgcgaaccca
    18121 ggcggcagga agtaccagga tttcagctcc ctgtaatgcc tgtgccagcg ccagctctgg
    18181 aaagcgtaag tcataacagg tcatcagacc gaccttcatc ccctccacct cgagtaacgg
    18241 agcgatttca ttaccagcat caacacggcg tgattcctga atggcaaatg catcatagag
    18301 atgcaatttg gcataacggg cgacgatgtt tcctgcctgt agcgccacca gcatattcca
    18361 tgcgcgcccc ggcgttgaag gaacatgaat cgtcagaatt gtcgtcatca tgttacgttt
    18421 actttctcgc cgtaaacgtc cgaggaattc gccttccagc agctgtgctg atttaaccga
    18481 tagatctgca tcatgatcat cgcgcgccag caatgcttcc ggcaggacga acagcgatac
    18541 gtcgttttcc gccgcctgcg ccatcaacga ggcacaaatc tcagcgttct tttcccacac
    18601 agatgtaaca gcaaactgtc ctgcggcaac cagcattgcc acctcctgtc agagtcgtaa
    18661 aattcgccaa cgacgattat tcggcgttac acttatcact catacaaatc aaatagcagg
    18721 attttgcagt gttacaactt cttttagcag tttttattgg cggtggtacg ggaagcgtgg
    18781 cgagatggct gttaagtatg cgatttaacc cgctgcatca ggcgattccg ttggggacgc
    18841 tggcagcaaa cctgattggg gcattcatca taggaatggg attcgcctgg ttcagcagga
    18901 tgacgaacat tgatccagtg tggaaagtat taatcaccac cggattttgt ggcggtctaa
    18961 caaccttctc aacattttcg gcagaagtgg tgtttttgtt acaagagggc cgctttggct
    19021 gggcgttact gaacgttttc gtcaaccttc tggggtcttt tgccatgaca gcactggctt
    19081 tctggctgtt ttcggcctca accgcacact aaagaccaaa aaaaacccgc tgattaagcg
    19141 ggttttgaat tcttgctgac gtatcttaca gagcgattac gtttgcagca gaagggcctt
    19201 tggcaccgtt agtgatttcg aactctacgc gctgaccttc agcaagagtt ttaaaaccat
    19261 tagtctggat tgcagagaag tgtacgaaca cgtctttgct gccgtcttcc ggagtaatga
    19321 aaccgaatcc tttggactca ttaaaccact taacgttacc tttaatctta gacatcaaaa
    19381 ttacctttac atgaaaaata gacacaaatg ctgtgtcggt taccagtaca ccaattgtgg
    19441 tacgctttgt ccagtgcaac tttactaaaa agtgataaaa gtcgcgttat ttttgatgtt
    19501 acttatttta gctattgatt ttaaagaagt tactaaaact tcatttgtct caaaactgaa
    19561 agcgcatcca ggcaaagtac acattgccat tgttgtaggt acccggaatg taggtcatct
    19621 gaaaagtcac tgggccataa cccacggagg ccaatggcag tagaaccggg agagggatgt
    19681 aattccagtt atcgcgtgcc gttacgccag cggtgaatcc cagacctaaa tgaaaatttt
    19741 catccgccag cggtcgccag gtactttccc atccgtatcc ggcaatcggt tcccatttgt
    19801 tccacgagtc cttaaatgcc atggcataca ggccatgcca gtttcctttt tcatcccaac
    19861 gcgacaggcc aaaaccgcca ccccacggtc gctcgttata gcgatcggtt ttttctttgt
    19921 cgtaagcgaa acgtgcatgc caggtgatgg caggaatata taaatcataa tgttcaggct
    19981 gttgccaggt ttgtgcaata ttttctctaa acgttgtcat ccactcatct gcgttagcaa
    20041 aaactttacc aacgctgatt aactgaataa aaacaaagga aaagatagcg acatatttac
    20101 tcacgttcat ttgtgaccat aaaacattta tcaaaaatct actactagca tagcaaagct
    20161 acaattaaca t
//
LOCUS       DAPHDW010000075        20136 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.075, whole genome shotgun sequence.
ACCESSION   DAPHDW010000075 DAPHDW010000000
VERSION     DAPHDW010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 20136)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20136)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20136
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.075"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..301)
                     /locus_tag="RH057_002731"
     CDS             complement(<1..301)
                     /locus_tag="RH057_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129080.1"
                     /translation="MKIVFEVNGKKVPLKSLKYISKKDQLEVMKNWFFENFEDPANAC
                     PYESREGGYAYIYGGPYDASEELQSIFGQYVKSEYIEELVDELQTQCFDWSGNSNN"
     gene            complement(493..915)
                     /locus_tag="RH057_002732"
     CDS             complement(493..915)
                     /locus_tag="RH057_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HDV3129081.1"
                     /translation="MAKVFTQEEREKIKGQVVELVRQSGRETLRQLEVKTGATRYLMS
                     VLARELVASGDVCNSGYGLFPSEQARKDWQNARKKLSRAKLKKPSAVDPDLIWSLPDG
                     EIRRYDRRQNIICRECRKSEVMQRILSFYQGDVRYLLK"
     gene            complement(956..2026)
                     /locus_tag="RH057_002733"
     CDS             complement(956..2026)
                     /locus_tag="RH057_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer"
                     /protein_id="HDV3129082.1"
                     /translation="MANAWLRLWHDMPNDPKWRTIARVSGQPIATVMAVYIHLLVSAS
                     RNVTRGHIDVTTEDLASALDVTEEVIDSILQTMQGRVLDGDLITGWEKRQVLKEDNGN
                     ISQTAKSPAERKRAQRERERKREQNGDCHGASRNVTHMSRRVTTDKDTDKDTDQEDQN
                     TMVHGVKNATNQAGDVQTVNLGQPAGTTPEADSAYALKADSGAVQQVMTARPEQSHQL
                     QQPEADSAIQREADRVVPENTGQSVGRVDYPDVFEQVWREYPLRAGANPKKSAFSAWK
                     ARLREGVPPEAMLDGVRRYARYLAATGKTGTEFVQRATTFFGPDRNFENPWLLPVSGT
                     NNQRCVNHISEPDNEIPPGFRG"
     gene            complement(2098..2523)
                     /locus_tag="RH057_002734"
     CDS             complement(2098..2523)
                     /locus_tag="RH057_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HDV3129083.1"
                     /translation="MKIKHEHIRMAMNAWARPDGEKVPAAGITQAYFELGMTFPELYD
                     DSHPEALARNTQKIFRWIEKDTPDAVEKIQALLPAIEKAMPPLLVARMRSHSSAYFRE
                     LVETRERLVRDADDFVAVAIAGFNQMNRGGPAGNAVAVH"
     gene            complement(2507..2749)
                     /locus_tag="RH057_002735"
     CDS             complement(2507..2749)
                     /locus_tag="RH057_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129084.1"
                     /translation="MKKSEVLGYFGGVVKTAAALGTSKTTVSMWGEEVPWKWALLIQA
                     VTAGALKYELHIPTVVIPGSDHNPPSNQGGIHENQA"
     gene            3141..3479
                     /locus_tag="RH057_002736"
     CDS             3141..3479
                     /locus_tag="RH057_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310236.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase S24"
                     /protein_id="HDV3129085.1"
                     /translation="MKNSSITSCVQLVGEIPANTFAVVLESDSMSTSGGGVSIPNGST
                     VFVDPDRTVQPGNIVLALPKGTTTPVIRKLEIEGPDILLVPTNPRYPSIMLDDLSCIL
                     GVCFKIQQNI"
     gene            3772..3927
                     /locus_tag="RH057_002737"
     CDS             3772..3927
                     /locus_tag="RH057_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HDV3129086.1"
                     /translation="MDTIDLGNSESLVCGVFPNQDGTFTAMTYTKSKTFKTENGARRW
                     LERNSGE"
     gene            4087..4305
                     /locus_tag="RH057_002738"
     CDS             4087..4305
                     /locus_tag="RH057_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129087.1"
                     /translation="MQKREPVIIAPDYTDDELYEWMRQKINAAQDLKWANETRAKQAE
                     NLSALEQDITNLEKAAALSIARMITYPR"
     gene            4873..5061
                     /gene="dicB"
                     /locus_tag="RH057_002739"
                     /gene_synonym="ftsT"
     CDS             4873..5061
                     /gene="dicB"
                     /locus_tag="RH057_002739"
                     /gene_synonym="ftsT"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibition protein DicB"
                     /protein_id="HDV3129088.1"
                     /translation="MKTLLPNVNTSEGCFEIGVTISNPVFTEDAINKRKQERELLNKI
                     CIVSMLARLRLMPKGCAQ"
     gene            5058..5249
                     /gene="ydfD"
                     /locus_tag="RH057_002740"
     CDS             5058..5249
                     /gene="ydfD"
                     /locus_tag="RH057_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysis protein YdfD"
                     /protein_id="HDV3129089.1"
                     /translation="MNSAFVLVLTVFLVSGEPVDIAVSVHRTMQECMTAATEQKIPGN
                     CYPVDKVIHQDNNEIPAGL"
     gene            5343..7814
                     /locus_tag="RH057_002741"
     CDS             5343..7814
                     /locus_tag="RH057_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HDV3129090.1"
                     /translation="MSKVFICAAIPDELATREEGAVAVATAIEAGDERRARAKFHWQF
                     LEHYPAAQDCAYKFIVCEDKPGIPRPALDSWDAEYMQENRWDEESASFVPVETESDPM
                     NVTFDKLAPEVQNAVMVKFDTCENITVDMVISAQELLQEDMATFDGHIVEALMKMPEV
                     NAMYPELKLHAIGWVKHKCIPGAKWPEIQAEMRIWKKRREGERKETGKYTSVVDLARA
                     RTNQQYTENSTGKISPVIAAIHREYKQTWKTLDDELAYALWPGDVDAGNIDGSIHRWA
                     KKEVIDNDREDWKRISASMRKQPDALRYDRQTIFGLVRERPIDIHKDPIALNKYICEY
                     LTTKGVFENEETDLGTVDVLQSSETQTDAVETEVSDIPKNETAPEAEPSVEREGPFYF
                     LFADKDGEKYGRANKLSGLDKALAAGATEITKEEYFARKNGTYTGLPQNVDTAEDSEQ
                     PEPIKVTADEVNKIMQAANISQPDADELLAVSRGEFVEGISDPNDPKWIKGIQTRDSV
                     NQNQQETEQNDQKAEQNSPNTQQNEPETKQPEPVVQQEPEKICTACGQSGGGNCPDCG
                     AVMGDATYQEIFDGENQPEVQENDPEEMEGTAHQHKENTGGNQHHASDSETGEASDPL
                     IKANGHHNLTSTSRAGIHLMIDLETMGKNPDAPIISIGAIFFDPQTGDMGPEFSKTID
                     LETAGGVIDRDTIKWWLKQSREAQSAIMTDEIPLDDALLQLREFIDENSGEFFVHVWG
                     NGANFDNTILRRSYERQEIPCPWRYYNDRDVRTIVELGKAIDFDARTAIPFEGERHNA
                     LDDARYQAKYVSVIWQKLIPSQADS"
     gene            7887..8138
                     /locus_tag="RH057_002742"
     CDS             7887..8138
                     /locus_tag="RH057_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000005552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase family protein"
                     /protein_id="HDV3129091.1"
                     /translation="MSEVIMIVSPGKWVSEEQLIALKGIKKGTLKKAREKSFMEGREY
                     KHVAHDGMPWDNSPCFYNLEEIDRWIERQASARPRRHLT"
     gene            8173..9453
                     /locus_tag="RH057_002743"
     CDS             8173..9453
                     /locus_tag="RH057_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3596 domain-containing protein"
                     /protein_id="HDV3129092.1"
                     /translation="MKYPTGVENHGGKLRIWFVYKGVRVRENLGVPDTAKNRRVAGEL
                     RASVCYAIKTGVFDYAKQFPSSRNLEKFGEARQDLTIKELAEKFLALKETEVAKTSLN
                     TYRAVIKNILSIIGEKNLASSINKEKLLEVRKELLTGYQIPKSNYIVTQPGRSAVTVN
                     NYMTNLNAVFQFGVDNGYLADNPFKGISPLKESRTIPDPLSREEFIRLIDACRNQQAK
                     NLWCVSVYTGVRPGELCALGWEDIDLKNGTMMIRRNLAKDRFTVPKTQAGTNRVIHLI
                     KPAIDALRSQMTLTRLSKEHIIDVHLREYGRTEKHKCTFVFQPEVSARVKNYGDHFTV
                     DSIRQMWDAAIKRAGLRHRKSYQSRHTYACWSLTAGANPAFIANQMGHADAQMVFQVY
                     GKWMSENNNAQVALLNTQLSEFAPTMPHNEAMKN"
     gene            complement(9455..9583)
                     /locus_tag="RH057_002744"
     CDS             complement(9455..9583)
                     /locus_tag="RH057_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016244635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HDV3129093.1"
                     /translation="MTIEKHERSTKDLVKAAVSGWLGTALEFMDFKSHTCQLFDKY"
     gene            complement(9641..10660)
                     /locus_tag="RH057_002745"
     CDS             complement(9641..10660)
                     /locus_tag="RH057_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent oxidoreductase"
                     /protein_id="HDV3129094.1"
                     /translation="MKSILIEKPNQLSIIEREIPTPSAGEVRVKVKLAGICGSDSHIY
                     RGHNPFAKYPRVIGHEFFGVIDAVGEGVESARVGERVAVDPVVSCGHCYPCSIGKPNV
                     CTTLAVLGVHADGGFSEYAVVPAKNAWKIPEAVADQYAVMIEPFTIAANVTGHGQPTE
                     NDTVLVYGAGPIGLTIVQVLKGVYNVKNVIVADRIDERLEKAKESGADWAINNSQTPL
                     GEIFAEKGIKPTLIIDAACHPSILKEAVTLASPAARIVLMGFSSEPSEVIQQGITGKE
                     LSIFSSRLNANKFPVVIDWLSKGLIKPEKLITHTFDFQHVADAISLFEQDQKHCCKVL
                     LTFSE"
     gene            complement(10672..11886)
                     /gene="rspA"
                     /locus_tag="RH057_002746"
     CDS             complement(10672..11886)
                     /gene="rspA"
                     /locus_tag="RH057_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HDV3129095.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPQLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNW"
     gene            complement(12092..12418)
                     /locus_tag="RH057_002747"
     CDS             complement(12092..12418)
                     /locus_tag="RH057_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HDV3129096.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLTLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            12553..12894
                     /locus_tag="RH057_002748"
     CDS             12553..12894
                     /locus_tag="RH057_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HDV3129097.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            12929..13489
                     /gene="speG"
                     /locus_tag="RH057_002749"
     CDS             12929..13489
                     /gene="speG"
                     /locus_tag="RH057_002749"
                     /EC_number="2.3.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
                     /protein_id="HDV3129098.1"
                     /translation="MPSAHSVKLRPLEREDLRYVHQLDNNASVMRYWFEEPYEAFVEL
                     SDLYDKHIHDQSERRFVVECDGEKAGLVELVEINHVHRRAEFQIIISPEYQGKGLATR
                     AAKLAMDYGFTVLNLYKLYLIVDKENEKAIHIYRKLGFSVEGELMHEFFINGQYRNAI
                     RMCIFQHQYLAEHKTPGQTLLKPTAQ"
     gene            complement(13492..14202)
                     /locus_tag="RH057_002750"
     CDS             complement(13492..14202)
                     /locus_tag="RH057_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HDV3129099.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            14310..14615
                     /locus_tag="RH057_002751"
     CDS             14310..14615
                     /locus_tag="RH057_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HDV3129100.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYTRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            14814..16226
                     /locus_tag="RH057_002752"
                     /pseudo
     CDS             14814..16226
                     /locus_tag="RH057_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(16693..17679)
                     /locus_tag="RH057_002753"
     CDS             complement(16693..17679)
                     /locus_tag="RH057_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129101.1"
                     /translation="MTMTKDAFQFGIEPVRITDTDNIQINEGLPTNADPQVYALELAK
                     LVKTMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDAKSSTGFTQGTLQQDALNICDNVVRTINSRMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGTEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDIAYYNGEDRGVFMMAKRFTALSFSCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTETN"
     gene            complement(17763..18254)
                     /locus_tag="RH057_002754"
     CDS             complement(17763..18254)
                     /locus_tag="RH057_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129102.1"
                     /translation="MLFNNNNWKLSVTDINLYENTVSLDDQSYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQLNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGENLRSATHSAGRSKASIMADLDSGANWM
                     DCL"
     gene            complement(18874..19584)
                     /locus_tag="RH057_002755"
     CDS             complement(18874..19584)
                     /locus_tag="RH057_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129103.1"
                     /translation="MSQQVVIFNDEFSLSSYEFLTKVINPAREEAGENPVSNKDFINR
                     VKDELDLKEENFLLLDTGASGRKSSHTILNGDQLLLVGMRESKAVRRKVLEYIRRIEK
                     DKQLLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHRGLFRKNPISNEW
                     SVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGRPETIDKMKGILIAFTGTDQ
                     QKQEYLLKQAKSGKQKGL"
BASE COUNT         5485 a         4900 c         4722 g         5029 t
ORIGIN      
        1 tattatttga gtttccagac cagtcaaaac attgtgtttg taattcatct actaattcct
       61 caatatactc agatttaaca tattgaccga agatggattg tagttcttcg ctcgcatcat
      121 acggcccgcc atatatgtaa gcatagccac cttctctact ttcatacgga caagcatttg
      181 ccggatcttc aaagttttca aagaaccagt ttttcattac ttcgagttga tcttttttgc
      241 ttatgtattt taaagatttt agtggaactt tctttccatt aacctcgaaa acaattttca
      301 cgatgtaacg ctcccttgta tcaattgcgc aactattata ccacagcgaa atacagggca
      361 taacctgcct ccttaaacta acataccatg agccagcaat gtccgctatt ggcacaaagc
      421 ggacaaccac gctagctcta ccctgtgcca caaaatgtca atttgcatct gaactaatgc
      481 actttaatct cgtcacttca ataaataccg aacatcaccc tgataaaacg acaatatgcg
      541 ctgcataact tcactcttcc ggcactcgcg acagattatg ttctgacgcc tgtcgtagcg
      601 acgtatttct ccgtcaggta atgaccagat aaggtccgga tcaaccgcag atggtttctt
      661 cagctttgcc cttgagagct ttttacgggc attttgccag tccttacgcg cctgttcaga
      721 cgggaataac ccgtaaccag agttgcatac atcgccgctg gcaaccagct ctcttgcgag
      781 aacgctcatc agatatcttg tcgcacctgt cttgacttcc agttgccgta acgtctcacg
      841 cccactctgg cgtacgagtt caacaacctg tcctttaatt ttttcccgct cttcttgtgt
      901 aaaaactttt gccacaagcc ctcctgaaaa ttacctcatg accagaaatt aacacttacc
      961 ccctgaagcc cggcggaatt tcgttatccg gttcagaaat atgattcaca caacgctggt
     1021 tgttcgtgcc gcttaccggg agcaaccagg ggttctcaaa attccggtcc ggtccaaaaa
     1081 acgtcgtcgc tcgctgaaca aattccgttc ccgttttccc ggtagccgcc aagtatcttg
     1141 cgtaacgcct cacgccatcc agcatggcct ctggtggcac cccctcgcgt aatctggcct
     1201 tccaggcact gaaagcggat ttcttcgggt ttgccccagc acgcaacggg tactcccgcc
     1261 agacctgttc gaacacatcc ggataatcca ctcgtcccac agactgcccg gtgttttccg
     1321 ggactacccg atcggcttcc cgctgaatgg cggaatcggc ttcgggctgc tgcagttggt
     1381 gtgattgctc cggccttgcg gtcatcacct gctgcacagc gcccgaatcg gctttcagcg
     1441 catacgctga atcggcttcc ggtgtcgtgc ctgctggctg accaagattg acggtctgaa
     1501 catcccctgc ctggttcgtg gcgtttttta cgccatggac catagtgttt tgatcttctt
     1561 gatctgtatc tttatctgta tctttatctg tcgtgactcg tcgtgacatg tgcgtgacat
     1621 ttcgtgacgc gccgtgacaa tcgccatttt gttcccgctt tctttccctc tctcgctgcg
     1681 ccctcttgcg ctctgcagga gattttgcgg tttgcgaaat attgccgttg tcctctttaa
     1741 gcacctggcg tttttcccat ccagtgatta aatcaccatc aagtacccgc ccctgcatcg
     1801 tctgcaaaat tgaatcaatt acctcttctg tcacgtcgag cgcacttgcc aaatcttctg
     1861 tcgtgacatc aatgtgacct cgcgtgacat ttcgtgacgc gctcaccagg aggtggatat
     1921 acactgccat cactgttgca attggctgcc ctgacaccct ggcaattgtt cgccacttag
     1981 ggtcatttgg catgtcatgc cataatctga gccaggcgtt agccatactc acctcttctg
     2041 ataccgaatc tttttactca cgagttgccg gaagcgattc gatatggcta ttgtcagtca
     2101 atgtactgcc acagcatttc ctgccgggcc accacggttc atctgattga aaccggcgat
     2161 tgccactgcg acaaaatcat cagcgtctct caccagtcgc tcccgcgtct ccaccagctc
     2221 ccgaaaataa gctgaactgt ggctgcgcat tctggccacc agcaaaggtg gcattgcctt
     2281 ttcgatcgct ggtaacaacg cctgaatttt ttcaactgca tcaggggtgt ctttctctat
     2341 ccagcggaaa attttctggg tattgcgagc cagggcttcc ggatggctgt cgtcgtacag
     2401 ttcaggaaac gtcataccca actcaaaata agcctgggtt attccagctg ccggaacttt
     2461 ttcgccatca ggacgcgccc aggcattcat cgccatgcgg atgtgttcat gcttgatttt
     2521 catgaatccc cccttggtta gaaggcggat tatgatcaga accgggaatg acaaccgtcg
     2581 gtatgtgtaa ctcatatttg agcgccccgg cagtgactgc ctgaattagc aacgcccatt
     2641 tccacggaac ctcttccccc cacatgctga ctgtggtttt tgacgttcct agagctgcgg
     2701 ctgttttaac aactccgcca aaatagccta atacttctga ttttttcatg agtcgctcca
     2761 taaaactgaa cgccaaaagt ttaataatca aaaccaaaga aagtcaagaa acaaaaccat
     2821 ctgtgtttta aaatcaaaac atgaacaagc aaacaatatc tgaacgcata acccaacgta
     2881 tgcatgcgct aaacctgaaa ggcaaagacc ttgtcaatgc cactggcgca tcaaaaggct
     2941 ccgtaagtca atggatgaac ggtggaggag cgccgtcctc gcgttacata agttcactgg
     3001 caaaaatatt gaaagtaaac gaaaattggc ttcttaatgg aggagagtta aatacaggtg
     3061 attcgcttga tctatcttta ccgccgataa aaacggttcc gctactatca cttcagcagg
     3121 cagcaagctg gagtgattat atgaaaaatt cctcaataac ctcttgtgtg cagcttgtcg
     3181 gagaaatccc ggccaatacc tttgcagttg ttctagagag tgacagtatg tcaacatctg
     3241 gtgggggagt ttccatccca aatggttcaa cagtttttgt tgatcccgat cgaaccgtac
     3301 aaccaggaaa tattgtcctt gccttaccca aagggaccac aacacctgtc attcgtaaac
     3361 tggagataga agggccggat attcttttag tccccacgaa tcctcgctac ccttcaatta
     3421 tgctggatga tctatcttgc atattgggcg tatgctttaa aattcaacaa aatatttaac
     3481 cgacctcatc tatttgatta actgtatgcc atcgtggtga tggcttaaca gctgcctgct
     3541 taaaatgttt tgataaaaaa acattgacct gaaaagttca tttttctaaa cttcattcat
     3601 tccctctccc caccccacag aatgcagggc aatacttcta gttaccaggc agtggtcagg
     3661 ggttaagtag ccagcccgag gcgtaagaac atgacggcag ggttcaactt taataactat
     3721 gcagcaggtt tttgttccgc taccccggcg ttaaggggaa atgaggtcag catggatact
     3781 atcgatcttg gcaacagcga atctctggta tgtggcgtgt tccccaacca ggacggtacg
     3841 ttcaccgcga tgacgtatac caaaagcaaa acgtttaaaa ccgaaaatgg tgcccgtcgc
     3901 tggctggaaa gaaactcagg tgagtgatat ggatttcgac acaatcatgg aaaaggctta
     3961 cgaagaatac ttcgaaggtc ttgccgacgg cgaagaagct ctcagcttca acgaatttaa
     4021 acaggcgctt tccagttcgg caaaatctaa cggctgataa gcgaaacagc accgcgagga
     4081 atcagtatgc agaaacgaga acccgtcatc atcgcgccag actataccga tgatgaactt
     4141 tatgagtgga tgcgccagaa aattaatgca gcgcaggatt tgaaatgggc caatgaaacc
     4201 agggctaagc aggctgaaaa tctgtccgct ctggagcagg atatcaccaa tctggaaaaa
     4261 gcagcggcat taagcattgc cagaatgatt acatacccgc gttagtagct aatcaacaaa
     4321 gctaaggtta gtaattaagg agttctccac gggtgaggtg gagtgcgtgc gccggacacg
     4381 ggtgagcatc cggcactgac agtttactga aaggatattt ccctgaaaag tcagaccata
     4441 acgcgaaagc gcacggcgag gtagctggtt catagatagc ctgtcgttaa attttcgtcg
     4501 accgtgcgct tccggttgtg gcaacccgcg aaatggcgcg gcggtaagta tggcggggtt
     4561 attccttccc cgttgaggac accgggttgt caggttgacc atacgcttaa gtgacaaccc
     4621 cgctgcaacg ccctctgtta tcaattttct ggtgacgttt ggcggtatca gttttactcc
     4681 gtgactgctc tgccgccctt tttaaagtga attttgtgat gtggtgaatg cggctgagcg
     4741 cacgcggaac agttaaaacc aaaaacagtg ttatgggtgg attctctgta tccggcgtta
     4801 attgttaact ggttaacgtc acctggaggc accaggcact gcatcacaaa attcattgtt
     4861 gaggacgcga taatgaaaac gttattacca aacgttaata cgtctgaagg ttgttttgaa
     4921 attggtgtca ctatcagtaa cccagtattt actgaagatg ccattaacaa gagaaaacaa
     4981 gaacgggagc tattaaataa aatatgcatt gtttcaatgc tggctcgttt acgtctgatg
     5041 ccaaaaggat gtgcacaatg aattcagcat ttgtgcttgt tctgacagtt tttcttgttt
     5101 ccggagagcc agttgatatt gcagtcagtg tgcacaggac aatgcaggag tgtatgactg
     5161 cagcaaccga acagaaaatt cccggtaact gttacccggt cgataaagtt attcaccagg
     5221 ataataacga aatcccggca ggtctttaaa acagttccgt aataaatatc cggtttcatt
     5281 cttatatgcc agcaatggca gggatttgtt catccttaaa tctgtcatga ggttaaaaca
     5341 aaatgagtaa agtctttatt tgcgccgcta ttcctgacga actggcaaca agggaagaag
     5401 gcgctgtggc tgtagccaca gccattgaag ctggcgacga acgccgtgct cgagcaaaat
     5461 ttcactggca gttcctggaa cattatccgg ctgctcagga ctgcgcttat aaatttattg
     5521 tctgcgagga taaacctggc ataccccgcc ctgccctcga ttcatgggat gctgaatata
     5581 tgcaggaaaa ccgctgggat gaggagtctg cttcttttgt cccggttgag actgaatccg
     5641 atccgatgaa cgtcactttt gacaagctgg cccctgaagt acagaacgct gtcatggtta
     5701 agttcgacac atgtgaaaac atcaccgttg atatggttat tagcgcacag gaattgttgc
     5761 aggaagacat ggcaacattc gacggacata tcgttgaagc gttgatgaaa atgccagaag
     5821 ttaacgccat gtatccggag cttaagttgc acgccattgg gtgggttaag cataaatgta
     5881 ttcctggtgc taaatggccc gaaattcagg cagagatgcg catctggaaa aaacgtcgcg
     5941 aaggtgaacg caaggaaacc ggaaaataca cgtctgttgt tgatctcgcc cgcgccagaa
     6001 ccaatcaaca gtacactgaa aattcaacag gaaaaatcag cccggtcatt gctgccattc
     6061 atcgcgaata caagcagaca tggaaaacac tggatgacga actggcctac gctctctggc
     6121 ctggtgatgt ggatgccgga aacattgacg gcagcatcca tcgctgggca aaaaaagaag
     6181 ttatcgacaa cgaccgcgaa gactggaagc gtatctcggc atcaatgcgc aaacagcctg
     6241 atgcccttcg ctacgaccgc cagactattt ttggccttgt ccgtgagcgt ccgatcgaca
     6301 ttcacaaaga tcccatagca ctgaacaaat atatctgcga atacctgacg acaaagggcg
     6361 tgtttgagaa tgaagaaaca gacctgggca ctgttgatgt tctccagtca tcagaaacac
     6421 aaactgatgc agtggaaact gaggtatctg atatcccaaa aaatgaaacc gcgccggaag
     6481 ctgaaccatc tgtagagcgt gaggggccgt tctatttcct cttcgcagat aaggacggag
     6541 aaaaatacgg tcgcgcaaac aaactttctg gtctggataa ggcactggct gctggcgcca
     6601 ctgaaatcac aaaagaagaa tattttgccc gaaaaaatgg cacatacacg ggcttaccgc
     6661 aaaatgtaga taccgctgaa gattcagaac aaccagagcc gataaaagtt accgctgacg
     6721 aagtaaacaa aattatgcag gcagccaata tcagccagcc tgacgccgat gaactgcttg
     6781 cagtatcacg tggtgaattt gttgaaggga ttagcgaccc gaatgatccg aaatggatta
     6841 aggggatcca gacccgcgat tctgtgaacc agaaccagca agaaacggaa cagaacgacc
     6901 agaaagcgga acaaaacagc ccaaatacgc aacaaaacga gccagaaacg aaacaacctg
     6961 aaccagtagt gcaacaggaa ccggaaaaga tctgcaccgc ctgcggtcag agcggtggcg
     7021 gcaactgccc tgattgtggt gcggtgatgg gtgacgcaac ataccaggaa atattcgatg
     7081 gagagaatca gcctgaagtt caggaaaatg atccggagga aatggaaggc actgcgcatc
     7141 agcacaagga gaacactggc ggcaatcagc atcatgccag cgatagtgaa actggcgagg
     7201 cgtcagatcc cttaattaag gcaaacggtc atcataatct cacatccacc agcagagcgg
     7261 ggattcatct gatgatcgac cttgaaacca tgggaaaaaa tcccgatgcc ccgattatct
     7321 caataggtgc aatatttttc gatccgcaaa ccggagatat gggaccggaa tttagtaaga
     7381 ctatcgatct ggaaactgct ggcggagtca ttgatcggga caccattaaa tggtggctta
     7441 aacaatcacg cgaagcgcaa tctgccatta tgaccgatga aatcccgtta gatgatgcac
     7501 tgttacaatt gcgggaattt atcgacgaaa actccggtga attttttgtt catgtctggg
     7561 gaaatggagc caacttcgac aacacgattt tgcgccgttc atacgaacgg caggagatcc
     7621 cctgcccgtg gcgttactac aacgatcgcg atgtacgcac aatcgttgag ctggggaaag
     7681 ccatagactt cgatgccaga acggctattc cattcgaagg tgagcgccat aatgcacttg
     7741 atgacgcccg ttaccaggca aaatacgttt cagttatctg gcaaaaactg atcccgagtc
     7801 aggctgattc ttaatgttca actgtcgccg gttgtgactg gtattctgca accggcgctc
     7861 gtctgatgta agagataaag aaatcgatga gcgaagtaat catgattgtc tctcccggca
     7921 aatgggtatc cgaagagcag ttaattgcgc tgaaaggaat aaaaaaaggt acgttaaaaa
     7981 aggcccggga aaaatcgttt atggaaggaa gggaatataa gcatgtcgct catgacggta
     8041 tgccatggga taacagtcca tgcttttaca acctggaaga aattgatcgc tggattgagc
     8101 gccaggcatc agcgagacca agacgtcatc ttacttgact aaaagccaca ctaactaatg
     8161 agagaagttg aaatgaaata tccgacaggc gtggaaaacc atggagggaa attacgtatc
     8221 tggtttgttt ataaaggcgt aagagtcagg gaaaatctgg gggttcctga cacagcaaaa
     8281 aacaggcgcg ttgcaggtga actacgcgcc tctgtttgtt acgcaataaa aactggtgtt
     8341 ttcgactatg caaaacagtt tccctcctca cgcaatctgg aaaaatttgg tgaggcccga
     8401 caagatttaa ccataaaaga actggctgaa aaatttctgg cactgaaaga aactgaagtc
     8461 gccaaaacat cactcaacac ataccgtgcc gtcatcaaaa atatcctgag cataatcggt
     8521 gaaaaaaatc ttgcctcatc gattaataaa gaaaaattac tggaggttcg taaagagtta
     8581 ctgactggat accagatccc caaaagtaac tatattgtta cacaaccagg gagatcggct
     8641 gtaactgtaa ataattacat gacaaatctt aacgccgtgt tccagtttgg tgttgataac
     8701 ggttacctgg cagataatcc gtttaagggg atctcgccat taaaggaatc aagaaccatt
     8761 ccggatcctc tttcgcggga agaatttatc cgtcttatcg atgcgtgcag aaatcagcaa
     8821 gcaaaaaatt tatggtgtgt ttctgtttat actggcgttc gccctggtga gctgtgtgca
     8881 cttggatggg aggacataga tctgaaaaat ggaacaatga tgatcaggag aaatttagca
     8941 aaagaccgtt tcacggtacc aaaaacacag gcgggaacca atcgggtcat tcatcttatt
     9001 aagccagcaa tcgacgctct ccggagtcag atgacattaa cgagactgag caaagagcat
     9061 atcattgatg ttcacctcag agagtatggc agaacagaaa aacataaatg cacctttgtt
     9121 tttcaacctg aagtgtcagc gagagtaaaa aattatggtg accattttac cgttgactca
     9181 ataaggcaga tgtgggacgc agcgataaaa cgtgccggac tccgccatcg aaaatcatat
     9241 cagtcgagac atacttatgc ctgctggtcg ctgacagctg gtgctaaccc ggcatttata
     9301 gcaaaccaga tgggccatgc agatgcgcaa atggtatttc aggtatacgg aaaatggatg
     9361 tctgaaaaca ataatgcaca ggtagctttg ttaaatacac agttaagcga gtttgcccca
     9421 accatgcccc ataacgaagc aatgaaaaat taatttaata tttatcaaat agttgacacg
     9481 tatgactctt gaaatccata aattcaagcg cagtgcccag ccatcccgat actgctgctt
     9541 tcaccaaatc cttagtgctt ctttcgtgtt tttctattgt cataatggtt atctctaaaa
     9601 aagaggtaag atgcgtacta cttactcgcc gttattggta ttattcagaa aaagtgagta
     9661 agactttgca gcaatgtttt tgatcctgtt caaataaact aatggcatca gcaacatgct
     9721 ggaaatcaaa cgtatgggta attaattttt ctggtttaat taacccttta cttaaccagt
     9781 cgataacaac cggaaattta tttgcattta agcgtgaaga gaaaatagag agttcttttc
     9841 cggtaattcc ttgctgaatc acttcagacg gttcactgga gaagcccatc aatacaatac
     9901 gtgccgctgg agaagccagc gttacggcct ctttcaggat agaaggatga caagccgcat
     9961 cgataattaa tgtcggcttg atgccttttt cagcgaaaat ctcgccaagc ggtgtctggc
    10021 tgttattaat cgcccagtct gccccgctct ctttcgcttt ttccagtcgt tcatcaatgc
    10081 gatcggcaac aatcacattt ttaacgttat agacgccttt taatacctga acgatcgtca
    10141 ggccgattgg accggcacca taaaccagaa cggtatcatt ttcagtcggt tgaccatgac
    10201 cggtaacgtt agccgcaatg gtaaaaggtt cgatcatcac cgcatattga tcggccactg
    10261 cttcaggaat tttccacgca ttttttgccg gaaccacggc atattcactg aaaccaccgt
    10321 cagcgtgcac acctaataca gcaagtgtcg tacaaacgtt cggcttacct atagagcacg
    10381 gatagcaatg cccacagctg accaccggat cgacagcaac gcgttcaccg actctggcgc
    10441 tttccacacc ttcacccacc gcatcaatga cgccaaagaa ttcatgacca atgacgcgcg
    10501 gatatttcgc aaaaggatta tgcccacggt aaatatggct atctgaacca caaattccgg
    10561 caagtttcac ttttactcgt acttcacccg ctgacggggt gggtatttca cgttcgataa
    10621 tcgacagttg attcggtttt tcaattaaaa tgcttttcat taccttactc cttaccagtt
    10681 ccacagcgtg ccatcttcca gacgtgcgac tggcagataa gcaggttcat agggatattt
    10741 cgccgccagc ttttcatcga attcgatgcc aagacccggt ttgtctcccg gatgcatata
    10801 gccgttatcg aaagtccagt tgtgcgggaa gacttcgagc atttgttcgg aataacccat
    10861 gtactcctgg acaccgaaat tggggaccca cagatcaaag tgcagcgccg cagccatgca
    10921 gactggtgac aaatcggaag gaccgtgtga gccagtacgt acctgataca gcgaagcaaa
    10981 atcggcaatc cggcgcatac cggtaattcc gcctgcatgg gtcagcgtgg tgcggatata
    11041 atcgatgagt tgctcttcaa tcagttgttt gcagtcccag atgctgttga agacttcacc
    11101 cactgcgatg ggtgtgacgg tatgttggcg aatgagacgg aagcattcct ggttttccgc
    11161 aggcgtcggg tcttccatcc agaacatgcg ataatcttca atgcttttac caaagcgcgc
    11221 cgcttcaata ggcgttaagc gatggtgcat gtcatgcagc aaatgttcat taaaaccaaa
    11281 cttgttacgt accgcgtcaa acaatttcgg catgaaatcg aggtatttct ccgtcgacca
    11341 cagctgctct tccggccact gtcctttggt tgcgggttca taagccagac ctttaccttt
    11401 cgacatgccg taggtggttt tcataccagg gattccgcac tgcacgcgga tggctttgaa
    11461 tcccagctcc tgatgacggg cataatcatc cagagcttca tcaatactgt gaccggtggt
    11521 atggcaataa accatcaccc cttcacgaga cgcgccgccg agtaactggt aaagcggcat
    11581 gttggcagct ttggctttaa tatcccacag cgccatatca accgctgaaa tggccgacat
    11641 cgtaaccgga ccgcgacgcc agtaagcacc tttatagaaa aactgccaga tatcttcgat
    11701 acggtgcgca tcgcgaccaa taagctgcgg acaaaggtga tcctgcaaat aagaggccac
    11761 ggaaagctca cgtccattga gggtggcatc cccaaggccc gtaataccgt cctcagtggt
    11821 gatttttaat gtgacgaaat tacgccccgg acaggtaaca aaaacttcag cctttacgat
    11881 cttcatgttc gattccttgc atcgcttgtc gtgatgcatg aaatctacgc aactgagcta
    11941 ctaccataca agtatgaaga tcgaaaaaag ccggagtgat cacaaaaaaa ggcgtatatt
    12001 tgcgctgtga atggttgaca aaagatgaaa tagaatacct tttgtcagct gacacttcct
    12061 cttatcttat tgataaaatg gatttatgtt cctacgtgcg cccccagccc gcaacaatga
    12121 tcaacatgcc gcaaagcgca atcaacgcac ccgtccagtc ataaagagtc agtttcacgc
    12181 catccacaac gcgcagccac atcaacgccg tgcagacata aacgccacca taagccgcgt
    12241 aaacacgccc actcgccgct ggatgcaacg ttaacaacca gacaaacagc gccagtgaaa
    12301 tccccgccgg aagcaacagc cagatactgg cgtttcgttt taaccacaac cagggcagaa
    12361 agcatccaat aatttcacac agcgcagtag caaaaaatag taacgttgtt ttaatcatct
    12421 ttgtcactta ttgacatcat gtatagttat agggcgacat aatatcatca atataaacac
    12481 cctcctggta cgttttgcgt tcgcagtgga tggtgtagaa tctcctttaa tcattcatac
    12541 agggaatgaa ttatgaaaat cactctcagc aaacgaatcg gcctgctcgc ttttctgctg
    12601 ccttgcgcac tggcactgag cacaactgtt catgccgaaa ctaacaaact ggtgattgag
    12661 tctggcgaca gtgcacaaag ccgccagcac gccgctatgg aaaaagagca atggaatgac
    12721 acgcgcaatc tgcgtcagaa agtgaataaa cgcactgaaa aagagtggga taaagccgac
    12781 gccgcttttg ataaccgcga taaatgtgag caaagcgcca acatcaatgc ctactgggag
    12841 ccaaatactt tgcgctgcct ggaccgtcga actggccgcg ttattacccc ctaacctgtt
    12901 attgatttaa ggaatgtaag gacacgttat gccaagcgcc cacagtgtta agctacgccc
    12961 gctggagcgt gaagatttac gctatgtaca tcaactcgac aataacgcca gtgtgatgcg
    13021 ttactggttt gaggaaccct acgaagcctt tgttgaactc tctgatctgt atgataagca
    13081 tattcacgat cagagcgaac ggcgctttgt ggtggaatgt gacggcgaaa aagccggtct
    13141 ggtggagctg gtggaaatta accatgtaca tcgccgggca gaatttcaga taattatctc
    13201 cccggagtat caggggaaag gtctggcaac ccgtgccgcc aaattagcaa tggactatgg
    13261 ctttaccgtt ctcaatctct ataagctgta tctgatcgtt gataaagaga atgaaaaagc
    13321 gattcacatt taccgcaagc ttggcttttc ggttgaaggt gaattgatgc acgagttctt
    13381 tattaatggt caatatcgta atgccattcg catgtgtata ttccagcatc agtatctggc
    13441 agagcacaaa acaccgggtc agactctcct gaagccgacc gcacaatagc attaataata
    13501 atcgatcgta tttttgatgg tgtaaacccg ttcgacggcg ggttttactc cttcatcaac
    13561 aatgattagc tgacagtcca ccggattagc gtgactgtca tattcacagc tctgttttac
    13621 attaccaacc cgttgattat tcagtaaagt aaccgctgtg taatctaatt ttttgatcgg
    13681 atccgttgat ggcgtggcgc tgacagataa tgttttgtcg ttacttttcg tggttttacc
    13741 cagcggataa ccctgatcat catagcgata ttccatctgc atttgtttgc tgctggcttt
    13801 aatcacgaag ccattatcat cggtttccca actcaccccg gcagaaggta attctgctag
    13861 ctggcatttt ccctgtaaac gtactctctt ctccagcgtc tcggcatcac ggtaataatt
    13921 ggcgtccagt accagagcga ccacggtatt attttccaga tccagtaatt cgagtgaatc
    13981 aaaacagcct tcttccgaca aagtcccaga aacacgtttc gtcacttcac cttgctcatc
    14041 cattaatgtc tgagtgaaat cttttaccgg accacgcagc ggatcaaaat cgaattcatt
    14101 agagaaactg gccatctcag gggtaaatga aagtgttacc tctgtgcggt cacatcctgt
    14161 gaggaatatc gcgagtaagc atggtagtaa tttgtatttc acaacagtca ccagagagta
    14221 gagatgattc tcaatcatag tagcaaatac agtactttac acgttaaatg ctatgcttaa
    14281 agaagttatc ttcgcgtaag gagcttatga tgaaactctc aacctgctgt gccgcgcttc
    14341 tgctcgccct cgcctcaccc gcggtactcg ccgcgccggg atcctgtgag cgcatacaga
    14401 gcgatatatc acagcgcatt atcaataatg gcgtacccga aagcagcttc acgttaagta
    14461 ttgtacccaa tgaccaggtt gatcagcctg attcccaggt cgtcgggcat tgtgctaatg
    14521 atacgcataa aattctctac acccgcacca ccagcggtaa cgtctctgct cccgcgcagt
    14581 ctacccagga tggcgcgcct gccgaaccgc agtaatacat tatcgactga acgccggata
    14641 cgacacatcc ggcgatttga acatacaaca taatcccacc ttattactca tacccttcta
    14701 ttgatatgga ttaataattc ttaacccaaa atgggtagac tccctctatt gttagcgcgc
    14761 taaatattca atatataaac atttatataa cgataaagaa cagggagtga gttatgtcca
    14821 aaaatgaccg aatggtgggc atcagccgca gaacactcgt taaatcaacc gcgataggtt
    14881 ctctggcgct ggctgcaggc ggtttttctt taccgttcac cctgcgcagt gcagcggcag
    14941 cagtacaaca ggccagcgaa aaagtgatct ggggtgcctg ttccgtcaac tgtggtagcc
    15001 gctgtgcact tcgtctacat gttaaagata atgaagtcac ctgggtggaa actgacaaca
    15061 ccggcagcga cgagtatggc aatcatcaag tacgcgcctg tttgcgtggt cgatccatcc
    15121 gccggcgtat taatcatccc gatcgcttga attacccaat gaaacgcgtg ggtacacgcg
    15181 gtgaaggcaa attcgaacgg attagctggg atgaagccct ggatactatc gccagtagcc
    15241 tgaaaaaaac cgtcgaacaa tatggcaatg aggctgtata tattcagtac tcttcgggga
    15301 ttgttggcgg caatatgacc cgctcttcgc catcagcctc ggcggtcaaa cgcctgatga
    15361 actgctacgg cggttcactc aatcagtatg gctcctacag caccgcgcaa atttcctgtg
    15421 ccatgcccta cacctacggc agtaatgatg gcaacagcac cacggatatt gaaaacagca
    15481 agctggtcgt gatgtttggt aacaacccgg cagaaacccg catgagcggc ggtggcatca
    15541 cttatcttct tgaaaaagcg cgcgagaaat cgaacgccaa aatgattgtt atcgatccgc
    15601 gatataccga tacggctgct ggtcgtgaag atgaatggct ccctattcgc ccgggcaccg
    15661 atgccgcgct ggttgcgggt attgcctggg tattgattaa cgaaaatctc gttgatcaac
    15721 cttttctcga taaatactgc gtcggctatg acgaaaaaac cttacccgca gatgcaccca
    15781 aaaatggtca ctataaagcc tatattcttg gtgaaggtga tgataacaca gcgaaaacgc
    15841 cgcagtgggc ttcgcaaatt accggtatcc cggtagaccg tatcatcaaa ctggcgcgtg
    15901 aaattggcac agcaaaaccc gcttatatct gccagggctg ggggccgcaa cgccaggcaa
    15961 acggcgaact gactgcacgc gctattgcta tgttacctat tttgacgggc aatgtcggca
    16021 tcagcggcgg aaatagtggc gcgcgtgaat cgacctatac cattaccata gaacgcctgc
    16081 cggtgctgga taatccggtc aaaacgtcaa tctcctgctt cagctggaca gatgctatcg
    16141 atcatggtcc gcaaatgacg gcaatccgcg acggcgtccg cggcaaagat aaactggatg
    16201 tgcccattaa gttcatctgg aacttcaagg acttgaagcg gacccttagt aggcctgagg
    16261 tcctgcattg agtcaagacc ttgtgtcatt ctttgtgcca gacttgtgtc attagacggg
    16321 atgtctagac ggctggtcag gaccctccgg tggccttggt atggacttga ggcgggacta
    16381 tgggggaagt ctgctggctt aatgtattag atggtcattc acatttttct gtcagaaacc
    16441 atcggtccca ctgtgagtcc aaacctaagg acctagaagt ccacccacag ccttagacag
    16501 agtgtcgaac acatccacac cgagtcgacc agagaggacc atgtagagac ccacagagag
    16561 gaacacgagt ttatagacca gagtcaggag ggacttcagg tctgcggaag tcatagacat
    16621 ttggccctcc ttatcttgta tactaccata ccagaaataa ggcccagacc cgaaggtcca
    16681 gaccatagtt agttagttgg tctcagtaaa gctcaggcgt ttgactacag gcaggacagc
    16741 agtagcacta ccagtaccca tcttgattgt cgtagcagct tcagcgacca cgaagacctt
    16801 gccgaggatt tctaggtcgt gagactgaga gtcgccatct ctggataaga ccacggtaat
    16861 gttctcacca tcatgacgga agcagctgaa gctcagggcc gtaaaacgct tagccatcat
    16921 gaacacacca cggtcctcac cgttgtaata cgcgatgtcg gtacccagca tatccacgat
    16981 agcgtcttcg ccaccgaagc cctgcttagc agccagacgg gagagagcct tgaggtgtga
    17041 ctcatgggcg ataatgccgt agtcagaaac ctcagtaccg aaagaagact cggtgttctc
    17101 atacagggca tccatgatag cgtcagcctt agcggtcagg ctaccagtca ggaccggaag
    17161 ctctacagtc ttcaacagct tggaggattc cagtacatcg accatgcgac tattgatggt
    17221 acggaccacg ttatcgcaga tgttcagagc gtcctgctgg agcgtcccct gagtgaaacc
    17281 agtggaagac ttagcgtccc acagcttggt ttgatgtgat acgaggatag cctgttcaat
    17341 cggctccacg gtgatttcag tgccggacgg agcggtcacg acttcaggtc gcttgccacc
    17401 acggacaggg accagcacag agcggtcagc cagagtgtga gcaatgattg gagtcggtaa
    17461 gctgttacgg gtcggcagga cttctacagg gaacgggatg ttatcttgag cgtctttaag
    17521 cacaccattg agcatcgtct taaccagctt agccagctca agggcgtaga cctgagggtc
    17581 tgcgttagtt ggcaaacctt cgttaatctg aatgttgtcg gtgtcggtga tacgtactgg
    17641 ctcaatgccg aactggaaag cgtctttagt catagtcatg ataattaatc tccttgagtc
    17701 aaatacttga agtccctaac ggtaagtcag ggaccgtgtg ttcttagatg agggtcgctt
    17761 gcttacaagc agtccatcca gttagcgcca ctatcgaggt cagccataat tgaagccttg
    17821 gaacgaccag cggaatgggt agcactgcgg aggttctcac cataggtccg gcgttcagct
    17881 ttgacctgtt cacggcgctg tagctctttc tgccactctg catcggtcat gttctcttca
    17941 cgcatacgct cagcctgaag tcgttgctct ttagcgatac gctcagcctc agctcgtttg
    18001 ttaagctggc ggcgaccata ggcggtcatc aggtcattac tgaagaagtt accaatggtc
    18061 acgccagctt cagcaagagc ttccagtaac tccattgctt cgcggctggt ggatggcaag
    18121 cctgacagat accccggaat gagggtcttg atagcgaggg acagcggata ggactgatcg
    18181 tccagactca cagtattctc gtagagattg atgtcggtga ctgacagctt ccagttgttg
    18241 ttgttgaata acataagttg gtactcctct tgagaaggtg agaactatcg gtatcgcaga
    18301 cacagttatg cctacgggtc tccccgcatc ggtcattctc aaaaggtgtt taaattaagg
    18361 tattgagtag gtgagctgct aacggttagg agacagctag cttactagcg acagtttcat
    18421 agcgaccagt tgcgctatca gctcgtctcg ggattggtgc tggcgggtct catcttccgg
    18481 gtctagacca agctccttca ggaggaattc agtccgatag ccacggtaac ggagagtcat
    18541 gcgaccctca ggtgtgcgcg ggtcaaccat aggtctttgg gagaccatag gtctttggtt
    18601 agtcatcatt gttagaagtc ctcgttagac caatcatcca cgtcaaattc actggtgata
    18661 ggcgcttgga agtcaggtag accctctggt atatctagcg gcagtcgctc gtcgactctc
    18721 agtgccatct cttcgtatat atgccgaagc agagaagtca gtaagatatg gtccgcagga
    18781 tgctcatggt cgtatctctc aatgaaacat atggttgtcg ctattgactt cataatcagt
    18841 tcatcagtaa gtcggttgga atccatagtc atcttagagt cccttctgct tgccggattt
    18901 ggcttgcttc aggaggtatt cctgcttctg ctggtcggtg ccagtgaatg caataaggat
    18961 gcctttcatc ttgtcaatcg tctcaggtct accgaagacc tccagtccct tagcgttgaa
    19021 gcgaaagtta taaccacctg ctgagaactt ctggtctgac ggcttaatga tacggaagca
    19081 gtcggaatac tttggattta cagaccactc gttactgatt ggattcttac gaaagagacc
    19141 acgatgctga agaagtgcgg tcattagctt ctgagcaggt agaccatagg ttttgcagta
    19201 gtcaccccaa gtcacgcctc gacggttagc ggactgaatt gctgctcgct tcttctggtc
    19261 ttccagtaac tgcttgtcct tctcaattcg tcggatgtat tcaaggactt tgcgacgcac
    19321 agctttagac tcacgcatac cgaccaatag tagttggtct ccgttaagga tagtgtgaga
    19381 ggatttacgt ccagaagcgc ccgtgtctaa taaaaggaaa ttttcctctt ttaaatccag
    19441 ctcatccttc acgcgattga tgaagtcctt attactgacg ggattctccc cggcctcttc
    19501 acgggcagga ttgataacct tggtcaggaa ctcataggaa gacagagaga actcatcgtt
    19561 aaagatgact acttgttgag acatatcgtt gtactccttt ttgttatgcc tcctgaagtc
    19621 acaccctgag agcctactca agcccccaac gatagggaga ctcacaggca acaggagaca
    19681 tacagaaaga cttgaggtga tgatgaggtc gtgtgaagac cagagatgat ggtggtggtg
    19741 gtgactgctc agttttgagc gcgttaagtt cttcgaccat agagacgaag gttgcaagac
    19801 cgcatgtcag gacgagctgt aaggactcca gatggtgagc ggtgatagac ttaagccaat
    19861 gtagtgagta gacgagctac taactgagat gactgaagaa cgctaacgag ctggaggtcg
    19921 atacacgagg tctgacagag gcggtcttcc taccagtgat agttaagccc taccactggt
    19981 catacatacc caacctcaag caacgaccca atgtatacag ggtcccgatg ctttgagctg
    20041 gagactgacg gagctatcgt cgaccccatg gttgaggaag acacatagaa ggaatgaggt
    20101 ctgacttaag tctggactat aggtcttata ttaatc
//
LOCUS       DAPHDW010000076        19453 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.076, whole genome shotgun sequence.
ACCESSION   DAPHDW010000076 DAPHDW010000000
VERSION     DAPHDW010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19453
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.076"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(326..454)
                     /locus_tag="RH057_002756"
                     /pseudo
     CDS             complement(326..454)
                     /locus_tag="RH057_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            526..708
                     /locus_tag="RH057_002757"
     CDS             526..708
                     /locus_tag="RH057_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein"
                     /protein_id="HDV3129104.1"
                     /translation="MNHPVIGVVTKADLASMEQISLVKSWLREAGAHNVLVTSAVNNN
                     GVTELFALLHTEEGCC"
     gene            complement(809..1138)
                     /locus_tag="RH057_002758"
     CDS             complement(809..1138)
                     /locus_tag="RH057_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF496 family protein"
                     /protein_id="HDV3129105.1"
                     /translation="METTKPSFQDVLEFVRLFRRKNKLQREIQDVEKKIRDNQKRVLL
                     LDNLSDYIKPGMSVEAIQGIIASMKGDYEDRVDDYIIKNAELSKERRDISKKLKAMGE
                     MKNGEAK"
     gene            complement(1310..2368)
                     /locus_tag="RH057_002759"
     CDS             complement(1310..2368)
                     /locus_tag="RH057_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HDV3129106.1"
                     /translation="MRADKSLSPFEIRVYRHYRIVHGTRVALAFLLTFLIIRLFTIPE
                     STWPLVTMVVIMGPISFWGNVVPRAFERIGGTVLGSILGLIALQLELISLPLMLVWCA
                     AAMFLCGWLALGKKPYQGLLIGVTLAIVVGSPTGEIDTALWRSGDVILGSLLAMLFTG
                     IWPQRAFIHWRIQLAKSLTEYNRVYQSAFSPNLLERPRLESHLQKLLTDAVKMRGLIA
                     PASKETRIPKSIYEGIQTINRNLVCMLELQINAYWATRPSHFVLLNAQKLRDTQHMMQ
                     QILLSLVHALYEGNPQPVFANTEKLNDAVEELRQLLNNHHDLKVVETPIYGYVWLNME
                     TAHQLELLSNLICRALRK"
     gene            complement(2566..3039)
                     /gene="sbmC"
                     /locus_tag="RH057_002760"
     CDS             complement(2566..3039)
                     /gene="sbmC"
                     /locus_tag="RH057_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor SbmC"
                     /protein_id="HDV3129107.1"
                     /translation="MNYEIKQEEKRTVAGFHLVGPWEQTVKKGFEQLMMWVDSKNIVP
                     KEWVAVYYDNPDETPAEKLRCDTVVTVPGYFTLPENSEGVILTEITGGQYAVAVARVV
                     GDDFAKPWYQFFNSLLQDSAYEMLPKPCFEVYLNNGAEDGYWDIEMYVAVQPKHH"
     gene            complement(3158..4324)
                     /gene="dacD"
                     /locus_tag="RH057_002761"
     CDS             complement(3158..4324)
                     /gene="dacD"
                     /locus_tag="RH057_002761"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase DacD"
                     /protein_id="HDV3129108.1"
                     /translation="MKRRLIIAASLFVFNLSSGFAAENIPFSPQPPEIHAGSWVLMDY
                     TTGQILTAGNEHQQRNPASLTKLMTGYVVDRAIDSHRITPDDIVTVGRDAWAKDNPVF
                     VGSSLMFLKEGDRVSVRDLSRGLIVDSGNDACVALPDYIAGGQRQFVEMMNNYAEKLH
                     LKDTHFETVHGLDAPGQHSSAYDLAVLSRAIIHGEPEFYHMYSEKSLTWNGITQQNRN
                     GLLWDKTMNVDGLKTGHTSGAGFNLIASAVDGQRRLIAVVMGADSAKGREEEARKLLR
                     WGQQNFTTVQILHRGKKVGTERIWYGDKENIALGTEQEFWMVLPKAEIPHIKAKYTLD
                     GKELTAPISAHQRVGEIELYDRDKQVANWPLVTLESVGEGSMFSRLSDYFHHKA"
     gene            4533..5960
                     /gene="sbcB"
                     /locus_tag="RH057_002762"
     CDS             4533..5960
                     /gene="sbcB"
                     /locus_tag="RH057_002762"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HDV3129109.1"
                     /translation="MMNDGKQQSTFLFHDYETFGTHPALDRPAQFAAIRTDSEFNVIG
                     EPEVFYCKPADDYLPQPGAVLITGITPQEARAKGENEAAFAARIHSLFNVPKTCILGY
                     NNVRFDDEVTRNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDG
                     LPSFRLEHLTKANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYIFTHRNKHKLM
                     ALIDVPQMKPLVHVSGMFGAWRGNTSWVAPLAWHPENRNAIIMVDLAGDISPLLELDS
                     DTLRERLYTAKTDLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNL
                     KILRENPQVREKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLP
                     ALDITFVDKRIEKLLFNYRARNFPGTLDYAEQQRWLEHRRQVFTPEFLQGYADELQML
                     AQQYADNKEKVALLKALWQYAEEIV"
     gene            complement(6003..6230)
                     /locus_tag="RH057_002763"
     CDS             complement(6003..6230)
                     /locus_tag="RH057_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HDV3129110.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(6244..7302)
                     /gene="yeeE"
                     /locus_tag="RH057_002764"
     CDS             complement(6244..7302)
                     /gene="yeeE"
                     /locus_tag="RH057_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HDV3129111.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISVQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYIFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHVSGLNQTLQHYSTEHNSIAETFNLSVWPLIAVL
                     LVITFWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGITSPTANILQFLVAGDVKYINWGVFLVLGIFVGSFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(7481..8839)
                     /gene="plaP"
                     /locus_tag="RH057_002765"
     CDS             complement(7481..8839)
                     /gene="plaP"
                     /locus_tag="RH057_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416518.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HDV3129112.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANFNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(8829..8891)
                     /gene="yoeI"
                     /locus_tag="RH057_002766"
     CDS             complement(8829..8891)
                     /gene="yoeI"
                     /locus_tag="RH057_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HDV3129113.1"
                     /translation="MGQFFAYATVITVKENDHVA"
     gene            complement(9106..10035)
                     /locus_tag="RH057_002767"
     CDS             complement(9106..10035)
                     /locus_tag="RH057_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416519.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3129114.1"
                     /translation="MKPLLDVLMILDALEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLALEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAI"
     gene            complement(10081..10905)
                     /locus_tag="RH057_002768"
     CDS             complement(10081..10905)
                     /locus_tag="RH057_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3129115.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGSGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGMVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLEPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(10988..11242)
                     /gene="yoeB"
                     /locus_tag="RH057_002769"
     CDS             complement(10988..11242)
                     /gene="yoeB"
                     /locus_tag="RH057_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HDV3129116.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
     gene            complement(11239..11490)
                     /gene="yefM"
                     /locus_tag="RH057_002770"
     CDS             complement(11239..11490)
                     /gene="yefM"
                     /locus_tag="RH057_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HDV3129117.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDIIE"
     gene            11773..11823
                     /gene="hisL"
                     /locus_tag="RH057_002771"
     CDS             11773..11823
                     /gene="hisL"
                     /locus_tag="RH057_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HDV3129118.1"
                     /translation="MTRVQFKHHHHHHHPD"
     misc_feature    11799..11922
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            11969..12868
                     /gene="hisG"
                     /locus_tag="RH057_002772"
     CDS             11969..12868
                     /gene="hisG"
                     /locus_tag="RH057_002772"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HDV3129119.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPGLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            12874..14178
                     /gene="hisD"
                     /locus_tag="RH057_002773"
     CDS             12874..14178
                     /gene="hisD"
                     /locus_tag="RH057_002773"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HDV3129120.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSAEEIAAASERLSDELKQAMAVAVKNIETFHTAQ
                     KLPPVDVETQPGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCNKVVLC
                     SPPPIADEILYAAQLCGVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETARQALSASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLTAAERLTAHKNAVTLRVNALKEQA"
     gene            14175..15245
                     /gene="hisC"
                     /locus_tag="RH057_002774"
     CDS             14175..15245
                     /gene="hisC"
                     /locus_tag="RH057_002774"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HDV3129121.1"
                     /translation="MNTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FQLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLDNWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIAPYPLSTPVADIAAQALSPQGIVAMRERVAQIIAER
                     EYLIAALKEISCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            15245..16312
                     /gene="hisB"
                     /locus_tag="RH057_002775"
     CDS             15245..16312
                     /gene="hisB"
                     /locus_tag="RH057_002775"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HDV3129122.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPQLLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVERYLAEQAMDRANSHVIGDRATDIQLAENMGINGLRYDREALNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            16312..16902
                     /gene="hisH"
                     /locus_tag="RH057_002776"
     CDS             16312..16902
                     /gene="hisH"
                     /locus_tag="RH057_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HDV3129123.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDVVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGIDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            16902..17639
                     /gene="hisA"
                     /locus_tag="RH057_002777"
     CDS             16902..17639
                     /gene="hisA"
                     /locus_tag="RH057_002777"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HDV3129124.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPEMVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            17621..18397
                     /gene="hisF"
                     /locus_tag="RH057_002778"
     CDS             17621..18397
                     /gene="hisF"
                     /locus_tag="RH057_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HDV3129125.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVQEVQKRGAGEIVLNMMNQDGVRNGYDLEQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            18391..18999
                     /locus_tag="RH057_002779"
     CDS             18391..18999
                     /locus_tag="RH057_002779"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HDV3129126.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWTKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKSADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQGLDLGEVIDNLKNRH"
     gene            complement(19038..19283)
                     /locus_tag="RH057_002780"
     CDS             complement(19038..19283)
                     /locus_tag="RH057_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001360320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV3129127.1"
                     /translation="MISCEIGTGTSFINQDKVSGCVIPPNDSQALVEAMNELWNNEET
                     SNRYGENSRRRFEEMFTADHMIDAYVNLYTTLLESKS"
     gene            complement(19360..>19453)
                     /locus_tag="RH057_002781"
     CDS             complement(19360..>19453)
                     /locus_tag="RH057_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase domain-containing protein"
                     /protein_id="HDV3129128.1"
                     /translation="RYLHHVLDVIADWPVNRVGALLPWRVTLPA"
BASE COUNT         4993 a         5077 c         4871 g         4512 t
ORIGIN      
        1 cctgcgccat ctgcagcttc tgcaccagaa ccggtgaatc tcactcaaca ggcttcattt
       61 ctcctgacgc cagcctgaat acagctggcg ttttcattta taaacagaaa ggaaaaccga
      121 taataatgga atggacgccc cgaccatgaa gcaacggtag gatattactg ataaaccttc
      181 cagtggaggt gcgttatggc cgttttaacg attagcattg acctgacaaa aaatgaattc
      241 caaatccacg gtctgggtag gaacagaaaa atctaagctc agaaaacgga ttaaagtaag
      301 cgtcaacgga gcaccgtatt gacgcttatt tattggtgag aactacgttc catggcagga
      361 gttcgtcaac acggttggag ggccattccg gcagtatgct cagaatatgg cgcagatacg
      421 cttccggatc gataccgttc agacggcagg tgcctgcttt tctggcaaca agcaggaatc
      481 tcagcgctga agcaggaatt agaggtaaaa acgccctacc gggccatgaa tcacccggtc
      541 attggagtag taaccaaagc agatttagcc agcatggaac aaatatcttt ggtgaaaagt
      601 tggttacggg aagctggagc gcacaacgtg ttagtaacca gtgcagttaa taacaatgga
      661 gttacagaac tttttgcctt gctgcataca gaagaaggct gttgttaatt tcgattggtt
      721 ctcggatgag aatgatgacg gtgatacaca gataagaatc aatgagcaat gagggttgcc
      781 gggcaaccct cattgaataa aacgggaatt acttcgcttc gccgtttttc atttcgccca
      841 tagctttcag ctttttggag atatcgcggc gttctttgga gagctcggca tttttgatga
      901 tgtaatcgtc aacgcgatct tcatagtcac ctttcatgct ggcgatgatg ccctggattg
      961 cttcaacgct catccccggc ttgatgtaat cgctcaggtt gtccagcagc aggacgcgct
     1021 tctggttgtc acggatcttt ttctcaacgt cctgaatttc acgttgcagt ttgttcttac
     1081 gacggaacag acgaacaaat tccagtacgt cctggaatga aggcttggta gtttccattt
     1141 ttatacccct gataatgtga gagtcggatt cgtttaagca accgctattc gttagggcca
     1201 acattactca tagctgccgc agatgacaat gcttttatcc cttttcacta tcataccctt
     1261 tatccttgct gaatcgaagc agcagcaaga tgattctgaa gttcaggaat tatttgcgca
     1321 aggcccggca aatcagattc gatagcaact caagctgatg cgccgtttcc atgttcagcc
     1381 acacataacc atagattggt gtttccacaa ccttcaggtc atggtggtta ttgagcaact
     1441 gacgcagctc ttccacagca tcgttcaatt tttccgtatt ggcaaaaacc ggctgcggat
     1501 taccttcgta cagcgcatga acaaggctca gcagtatttg ctgcatcatg tgctgggtat
     1561 cacgaagttt ttgcgcgttc aataacacga aatggctggg gcgcgtggcc cagtatgcat
     1621 tgatttgcaa ctccagcata caaaccagat tgcggttaat ggtctggata ccttcatata
     1681 tcgattttgg aatacgggtt tctttgctgg cgggcgcaat cagtccacgc attttcacgg
     1741 catcggtcag gagtttttgt agatggcttt ccagacgtgg gcgttcgagt aagttcggtg
     1801 agaatgcaga ttgatagacc cgattatact cggtcagact tttcgccagt tgaatgcgcc
     1861 agtggatgaa cgcccgttgt ggccagatac cggtaaacaa cattgccagt aaagagccga
     1921 ggatcacatc gccgcttcgc cataacgccg tatcaatttc acctgtcggg gaacccacaa
     1981 caattgccag cgtcacccca atcaataaac cttgatacgg tttcttgccc agcgccagcc
     2041 aaccgcaaag gaacatggcc gccgcgcacc agactaacat cagcggtaac gagattaact
     2101 ccagttgcag agcgataaga cctaaaatcg aacccaacac cgtaccgcca atacgctcaa
     2161 aggcgcgagg gacaacgtta ccccagaacg agattggccc cataatcacc accatggtga
     2221 ccagcggcca ggtgctttcc gggatagtaa acaggcggat aatgagaaaa gtgagcagga
     2281 atgccagcgc gacccgagta ccatgcacaa tgcggtaatg gcggtatacc cggatttcaa
     2341 acgggcttaa tgacttatcg gcacgcacgc agaaactccg taatgagatt aaaagcgctc
     2401 ataactcatc tgtcggccag aaggcgcgcc tctgaattat ccgacggaca aaccgggaac
     2461 agtgtaattt cccggcagga gcgaaaaaat tgaaaggcgc atcttactct tttcgctttc
     2521 aaaaaaaaga gtggtcatcg cgttaacaca ccgccctgag atgaattagt gatgttttgg
     2581 ctgcaccgca acatacattt cgatatccca gtacccatct tccgcgccat tgttcaaata
     2641 aacctcgaag cagggctttg gtaacatttc ataagcactg tcctgcaaga ggctattaaa
     2701 gaactgatac cagggtttag caaaatcatc accgactaca cgagctaccg ccaccgcata
     2761 ctgaccacct gtaatttctg tcagaatgac gccctcactg ttttcgggaa gcgtaaagta
     2821 acccggcacc gtcacgacgg tgtcgcagcg taatttttcg gcgggtgttt catctggatt
     2881 gtcgtaatag acagcaaccc actccttcgg cacaatattt ttgctatcta cccacatcat
     2941 caactgctca aagcctttct ttaccgtctg ttcccacggg ccaacgagat ggaaacctgc
     3001 aacggtacgt ttctcttcct gcttaatctc gtagttcatg acgcctccat tgatactgtt
     3061 tttatataca gtatagttgc aaaattaaaa ccacaaggaa tgagtgttga ttatgcgagc
     3121 agactcgcac tcctgccagt ctgctgcaaa agaaaggtca ggccttatgg tggaaataat
     3181 cactcaggcg agaaaacatg ctgccttccc cgacagattc cagggtaacc agcggccagt
     3241 tcgccacctg tttatcacgg tcgtaaagtt caatttcccc tacccgctga tgggcgctaa
     3301 ttggcgcggt gagttcttta ccatcaaggg tatatttggc tttgatatgt ggaatttcgg
     3361 ctttcggtag taccatccag aactcttgtt ctgttcccag ggcgatattt tctttatcgc
     3421 cataccatat gcgttccgta ccgacctttt tcccacggtg caaaatttgc accgtagtaa
     3481 agttttgttg cccccaacgc agtaattttc ttgcctcttc ctcacgacct tttgcgctgt
     3541 cagcacccat taccactgca atgagacgac gctgcccatc tacagccgaa gcaatgagat
     3601 taaacccagc accagaagta tgacccgttt tcaggccgtc aacattcatg gttttatccc
     3661 acaataaccc gttacggttt tgctgggtga taccgttcca ggtgaggctt ttctcactgt
     3721 acatatgata aaactcgggc tcgccgtgga tgatagcacg agaaagcaca gctaaatcat
     3781 aagccgagct atgctgacct ggcgcatcca ggccatgcac tgtttcaaaa tgcgtatcct
     3841 tgagatgcag cttctcggca tagttgttca tcatttcaac aaactgccgt tgcccaccgg
     3901 caatatagtc aggcagtgca acacaagcgt catttccgga atccacaatt aaaccacggc
     3961 ttaaatcacg taccgatacg cgatcgccct ctttcaaaaa catcagtgaa gaaccgacaa
     4021 acaccggatt atctttcgcc cacgcatcgc gccccacggt gacaatatcg tctggcgtaa
     4081 tgcgatgact atcgatagcg cgatccacga cataacccgt catcagcttt gtcaggctgg
     4141 cgggattgcg ctgttgatgc tcattacccg cggtgaggat ctgaccggtg gtgtaatcca
     4201 tcagtaccca ggacccggca tgaatctctg gaggctgagg tgaaaaagga atgttttccg
     4261 ccgcaaaacc agacgataag ttaaaaacga acaaagaagc agcaataata agacggcgtt
     4321 tcaacagcaa accctcagga gtttcaaata gctgttcttt ttacggaaat acttatgaac
     4381 tggctggaat aaagtgcaag aaaatgtgac taccctctca tttttatctg acatgatctg
     4441 ttgccactcg ctgccaaatt gtggcgctaa agctgattag cacggtgata tttgatactc
     4501 tggcagacag cagaaataac ggatttaacc taatgatgaa tgacggtaag caacaatcta
     4561 cctttttgtt tcacgattac gaaacctttg gcacgcaccc cgcgttagat cgccctgcac
     4621 agttcgcagc cattcgcacc gatagcgaat tcaatgtcat cggcgaaccc gaagtctttt
     4681 actgcaagcc cgcggatgac tatttgcccc agcctggagc agtattaatt accggtatta
     4741 ccccgcagga agcacgggcg aaaggagaaa acgaagccgc gtttgccgcc cgtattcact
     4801 cgctttttaa cgtaccgaag acctgtattc tgggctacaa caatgtgcgt ttcgacgacg
     4861 aagtcacacg caacgttttt tatcgtaatt tctacgatcc ttacgcctgg agctggcagc
     4921 atgataactc gcgctgggat ttgctggatg ttatgcgtgc ctgttatgcc ctgcgcccgg
     4981 aaggaataaa ctggcctgaa aatgatgacg gtctaccgag ctttcgcctt gagcatttaa
     5041 ccaaagcgaa tggtattgaa catagcaacg cccacgatgc tatggctgat gtgtacgcca
     5101 ctattgcgat ggcaaagctg gtaaaaacgc gtcagccacg cctgtttgat tatatcttta
     5161 cccatcgcaa taaacacaaa ctgatggcgt tgattgatgt tccgcagatg aaacccctgg
     5221 tgcacgtttc cggaatgttt ggggcatggc gcggcaacac cagctgggtg gcaccgctgg
     5281 cgtggcatcc tgaaaatcgc aatgccataa ttatggtgga tttggcagga gacatttcgc
     5341 cattactgga actggatagc gacacattgc gcgagcgttt atataccgca aaaaccgatc
     5401 ttggcgataa cgccgccgtt ccggttaagc tggtgcacat caataaatgt ccggtgctgg
     5461 cccaggcgaa tacgctacgc ccggaagatg ccgaccgact gggaattaat cgtcagcatt
     5521 gcctcgataa cctgaaaatt ctgcgtgaaa atccgcaagt gcgtgaaaaa gtggtggcga
     5581 tattcgcgga agccgaaccg tttacgcctt cagataacgt ggatgcacag ctttataacg
     5641 gctttttcag tgacgccgat cgcgcagcaa tgaaaattgt gctggaaacc gagccgcgta
     5701 atttaccggc actggatatc acttttgttg ataaacgaat tgaaaaactg ttgttcaatt
     5761 atcgggcacg caacttcccg gggacgctgg attatgccga gcagcaacgc tggctggagc
     5821 accgccgcca ggtcttcacg cccgagtttt tacaaggtta tgcagatgaa ttgcagatgc
     5881 tggcacaaca atatgccgat aacaaagaga aagtggcgct gttaaaagcg ctttggcagt
     5941 acgcggaaga gatcgtctaa tttgaagcct ccgccgctgg tacggcggag gtcaatgttg
     6001 agtcaggctt tttgaacggt gatactccac gctgcatcgc caatctgctg ataatcggtg
     6061 atggcatgcc cttcttctgc cgcccactgt gggattgctt cagttgcctg ggtgcaatca
     6121 aactcaatta ccaactcatc gccgctggcc atttctgcca gtgctgcttt tgcttctatg
     6181 agtggaaacg gacagacctg cgtcaccaca tccagctttt taatcgccat ctttacacct
     6241 tacttaattt gccgcagcag ttgccagtcg cgccttacgc tgcggtcgaa catacacaag
     6301 ccaggacgca gtccagactc cgagaatcat aaatacaagg ccaatccagc cctgccaggt
     6361 catcattgca gtcataacca gtccattacc gatagagcaa ccacctgcaa tactggcacc
     6421 gaagcccatc agtacaccac ctaacccgct acgtaatgtt gtttgtgcat cagctgcgcg
     6481 aacgcggaac tcacggctcg ctttggctgc aataaatgac cccacgaaga tccctaacac
     6541 caggaaaacg ccccagttaa tgtatttcac gtcacccgcg accagaaatt gcagaatatt
     6601 ggccgttggg gaagtgattc ctaacccaaa catgcgtccg gttgcttcac tcaggggcca
     6661 ggctaaaagc gcaatcaaac cgatgagtac agcagtgaca aagggatgcc agcgtttttc
     6721 aaacagaata tgagcgatcc cggttcggcg cggcggtaag gtcgcgactt tgagttttgg
     6781 cttcttcaac tctttcatca ccacccagaa agttatcacc agcaaaacgg caatcaacgg
     6841 ccacacagac agattaaacg tctcggcaat ggagttatgt tcagtactgt agtgctgcaa
     6901 ggtttgattt aaaccactga catgtggaga acgcatcacc gcactcatca ccatataagt
     6961 gaaaagcgcg atccaactgc cgatcaatcc ctcacccgcg cgataccagg tcccggtggc
     7021 acatccaccc gccagaacaa ttcccagccc gaagatatac ccacctataa cagtaccaag
     7081 ccacgggaac gccccggctt cgtaagtcag taggcccgcc tgaattaacg caaaaacccc
     7141 cacgctttgt accgaaattg caatcagcaa ggcgtaaaac atgcgattat ttttcgcgat
     7201 atacatatcg cgaaaaccac ctgtcaggca aaaacgccca cgctgcatga caaatcccag
     7261 cagagcacca caaattagcc cgcttaatat cattgaaaac ataaattaat taaccagatg
     7321 aatgttaatg gggaaattat tcatgactgg tggaatgcag accaataacc aaattgtcta
     7381 aattagaaca aatggttatt aatgaggatg tcgataaaaa agaataaaaa aaccggagcc
     7441 aaatattcat tcgactccgg tttattatta gaaaagatga ttacgctacg tcttcgtact
     7501 gcggaaccgg gttacggaaa ctcttggtga cgcaagccag gtaaatcaga ccgatagcag
     7561 cccagatcag acccagaacc attgagcttt cttccaggtt aacccacagc gcaccaacgg
     7621 tcagtgcacc acacatcggc aggaacagat actggaagtg atctttcagc gtcttattac
     7681 gcttctcacg gatccagaac tgcgagatga ccgacaggtt aacgaaggtg aacgccacca
     7741 acgcaccaaa gttaatcagc gctgtagcca ttaccaggtc gaagttgatt gccagcaggg
     7801 caatcgcgcc aaccaggatg atgttcatcg ccggagtacg ccatttcggg tgtacataac
     7861 cgaagaagct tttcgggaac acgccgtcac gacccattac gtacatcaga cgcgcaacac
     7921 ctgcgtgcgc cgccataccg gacgccagta cggtaatggt ggagaagatc agcgcaccaa
     7981 cctggaatgc tttacctgcc acgtacagca tgatttcagg ctgtgacgca tccggatctt
     8041 taaagcgaga gatatccggg aagtacagct gcaggaagta agttgcaaag atgaagatca
     8101 tgccgccaat cagcgcggtc aggaaaatcg cacgcgggat cacgcgctct gcatctttgg
     8161 tttcttccga caggttgctg atgccgtcaa agccggtaaa ggagaagcac aggattgtcg
     8221 ccccggtaat catcgggata acatgcgcat cgccagacca gaacggacga gtgctcgcca
     8281 gcgtaccagc gccttcacct tcaaatacgc cataaacaac catgcccaga atcaccgcga
     8341 tcagcactac ctgcaacacg acgattacag tgttgaagtt cgctacggat ttcagactac
     8401 gcaggttaaa ggcggtcata aaggccacca gcgccaccac aaacatccac gatgggatgg
     8461 aaggcaccag agcttcaaaa tagattttcg ccagcagaat gttgatcatc ggcgcgaaca
     8521 gatagtcgag cagagaagac caacccacca taaagccgac agtcgggcta atggatttct
     8581 gggcgtaagt atatgcagag ccagcagaag gatagcggcg aaccagcttc ccgtagctca
     8641 gagccgtaaa caggatcgca atcaatgcga acgcataggc tgtcggcaca tgaccatccg
     8701 tgaggcctga aacgatacca aaagtatcaa acagcgtcat cggctgcata taggcaagac
     8761 ccatcattac aaccggaact aacgtaagcg ttttacgcaa ttccacgcga gaggtgtttg
     8821 gagtaacgtt atgcgacatg gtcattctcc tttacggtga taaccgtcgc gtaagcaaaa
     8881 aattgcccca tttttttgga ttcctcagcg acaacaactg tcgattttta gtaaatatct
     8941 atccggtacg aagcccggcc tcttggtatg aatgattggt ttgaagcaaa aaataaccga
     9001 cgctgatgaa acgtcggttt ttagtcattt tttgacagcg gcgcattgtg cctaaatggg
     9061 ggggaaatga caagagaatg agaggcttgt cataataatt tttctttaaa tggctgattt
     9121 tccgtcatca gattttgcat aaacaccggc gatcgcacta tttgctaaaa tttcatctcg
     9181 ccaccagccg ctggcaagcc ctgcggtctg ttcgttccag ccaatccaga cgggttcaaa
     9241 gagagtttgg gcgaccactt tcttctcgat taacgcgcca ctatcgagaa aacgttgcgc
     9301 cagataacgg ggtaaatagc cgcagccgag gccgctaatt tgcagctcca gcttggtttt
     9361 aaaatcgaag acggtaatgg cttcttgttc gtcgagaagt tgtgaggccg tagaagcgcc
     9421 ggcctgcgca gtgtccccca ccacaatcgc ccgatatcgc ttaatgatac gacggtttaa
     9481 cggttcttct tccagggcta acggatgatg aggcgctacc gcaaagactt gttccagatc
     9541 gcccaaccgc gagaaaccaa actcactgga agatggtggc tcatgcatcg cgccaacaat
     9601 aatatccgct cgcccctgag tcagggcatc ccatgaacca ccgagtacgc cattgataaa
     9661 cttcaggcgc gtcacgctat gatgttgata aaaagcttca atgagtggcg caagcagaga
     9721 aaaaggaaac gtgtcgtcca cgccaatcac cagttcgttt tcccagcctt catgcagttt
     9781 gatcgcctgc ttttccagtt ctcgcacggt atgcagaact tcccgccctt tctctaataa
     9841 catttttccg gtgcgggtga atttagcgcg gtgtccgcta cgatcaagca attgaatatt
     9901 aaggtcgctt tccagcttgt gaacggtgta actcagagcg gacggcgtct tatagagttt
     9961 ggccgacgcc gccgcaaaac tgccttcttt ttctaaggca tcgaggatca tcaacacatc
    10021 cagcagtggt ttcatacacg cccccttgca gtgcatgcgg cgtcctgctg gcgtggtcgt
    10081 ttactccagc ggcattacca gcggatcggg atactggtat tcaaatccca gttcattaca
    10141 aatccgactg ccatcaataa tcttaccttt gccgctgtcc agactatttc tgaactgcgg
    10201 aggctccagc cccagtaaac gggccatctg cggatagaaa acattacgcg cagggtgagc
    10261 tggcgcacat atattataga tgtgtccgcc tttaggtgcc tgtaacaaca gagtgatagc
    10321 gccaataaca tcttctaaat ggactaaatt aacaccatgt tcaccatcag gcgcggtttt
    10381 tccggcaaag aagcgtccgg gatgacgccc cggtccgacc aggcccgcaa gacgcagaat
    10441 atcgaccgaa gtaccgggta aattgtgcag ccagtcttcg agttcttcta atactcgtcc
    10501 actgttggtt accggattac gcggggtagt ttctttcacc atgccttgcg catcgccata
    10561 gacagatgtt gagctggtaa aaataattcg gggaatacga tgggccagcg cgctatccac
    10621 taactcttgt accgcttgta aatagaactc atcgccggag ccgctacgac gtgccggaag
    10681 cgtaatgacc agtgcatcgg catccatcag ggcatccaga tcgtcagaat cgcaaactaa
    10741 ctcaggctcc atgcgaagca gatagctatc aatgccactc attcgggccg cttcgacacc
    10801 atcttgtgtg gttttactcc cggtgacttg ccagcctcgc gctgaaagtg acatcgccag
    10861 cggcatgcct aaccacccta acccgacaat tgcgaccttt ttcatccgtt atctcctgat
    10921 acaccttaga tctataaggc tacgctagcg tatcaaaact gacaattcat tctatgaatg
    10981 aatctgttca ataatgataa cgacatgctg caatgagcag tgaatcgtcg gtaaccgcgt
    11041 ataccagacg gtgctcctct gtaatgcgtc gggaccagaa acctgacaaa ttatgtttca
    11101 ggggttctgg cttcccctta ccttcaaatg gcgttctgcg ggtatctttg ataagttcat
    11161 tgatcttttt aacaattcgc ttatctgttt cctgccagta cagataatca tcccatgatt
    11221 cctcagacca gattagtttc actcaataat gtccttttct gttcctttgc ctgatttcag
    11281 gctatcgatt gagtccatca atctccgggc gttagcgggg gaacgcagta gataagccgt
    11341 ctcttccagc gagttgtatt cttcgagtga catcagaaca caagcctctc cattctgacg
    11401 agtaataagg atcggggcat gatcttcaac ggctttcatc attgttgccg acaaattctg
    11461 acgcgcttcg ctgtagctaa ttgtacgcat gtcaatctcc tcttttgtac agttcattgt
    11521 acaatgatgg gcgttaatta actatttatt aattagtttg tagatcaagg tattgtcagt
    11581 gagacgaaaa tccaggcttc gctatttttg gtgccgtcag cttagaggac agtcctctta
    11641 gcgccctcct ttccccgctc attcattaaa caaatccatt gccataaaat atataaaaaa
    11701 gcccttgctt tctaacgtga aagtggttta ggttaaaaga catcagttga ataaacattc
    11761 aaagagactt ttatgacacg cgttcaattt aaacaccacc atcatcacca tcatcctgac
    11821 tagtctttca ggcgatgtgt gctggaagac attcagatct tccagtggtg catgaacgca
    11881 tgagaaagcc cccggaagat caccttccgg gggctttttt attgcgcgat tgataacggt
    11941 tcagacaggt ttaaagagga ataagaaaat gacagacaac actcgtttac gcatagctat
    12001 gcagaaatcc ggccgtttaa gtgatgactc acgtgaattg ctggcgcgct gtggcattaa
    12061 aatcaatctt cacacccagc gcctgatcgc gatggcagaa aacatgccga ttgatattct
    12121 gcgcgtgcgt gacgacgaca ttcccggtct ggtaatggac ggcgtggttg acctcgggat
    12181 tatcggcgaa aacgtgctgg aagaagagct gcttaaccgc cgcgcccagg gtgaagatcc
    12241 acgctacttt accctgcgtc gtctggattt cggcggctgc cgtctttcgc tggcaacgcc
    12301 ggttgatgaa gcctgggatg gcccgctctc cttaaacggt aaacgtatcg ccacctctta
    12361 tcctcacctg ctcaagcgtt atctcgacca gaaaggcatc tcttttaaat cctgcttact
    12421 gaacggttct gttgaagtcg ccccgcgtgc cggactggcg gatgcgattt gcgatctggt
    12481 ttccaccggt gccacgctgg aagcaaacgg cctgcgcgaa gtcgaagtta tctaccgctc
    12541 gaaagcctgc ctgatccagc gcgatggcga aatggaagaa tccaaacagc aactgatcga
    12601 caaactgctg acccgtattc agggtgtgat ccaggcgcgc gaatcaaaat acatcatgat
    12661 gcacgcaccg accgaacgtc tggatgaagt catcgccctg ctgccaggtg ccgaacgccc
    12721 aaccattctg ccgctggcgg gtgaccaaca acgcgtagcg atgcacatgg tcagtagcga
    12781 aaccctgttc tgggaaacga tggaaaaact gaaagcgctg ggtgccagtt caatcctggt
    12841 cctgccgatt gagaagatga tggagtgatc gccatgagct ttaacacaat cattgactgg
    12901 aatagctgta ctgcggagca acaacgccag ctgttaatgc gcccggcgat ctccgcttct
    12961 gaaagcatta cccgcaccgt taacgatatt ctcgataacg tgaaaacgcg tggcgatgaa
    13021 gccctgcgtg aatacagcgc gaagtttgat aaaaccacgg ttaccgcgct gaaggtgtct
    13081 gctgaggaaa tcgccgccgc cagcgaacgc ctgagcgacg agttaaaaca ggcgatggcg
    13141 gtggcagtaa agaatattga aaccttccac actgcgcaaa aactgccgcc ggtagatgta
    13201 gaaacgcagc caggcgtacg ttgccagcag gtcacgcgcc cggtggcttc agtgggtttg
    13261 tatattcctg gcggctccgc cccgctcttc tcaacggtat taatgctggc aactccggcg
    13321 cgtattgcag gttgcaataa agtggtgctg tgctcaccgc cgccgattgc cgatgagatc
    13381 ctttacgcgg cgcagctgtg cggcgtgcag gacgtgttta acgtcggcgg cgcacaggcc
    13441 attgccgcgc tggcgtttgg tacggaatct gtgccgaaag tggacaaaat cttcgggccg
    13501 ggtaacgcct ttgtcaccga agcaaaacgc caggtaagcc agcgtctgga cggtgcggcg
    13561 atcgatatgc ccgcaggtcc gtcggaagtg ctggtgattg ctgacagcgg cgctacgccg
    13621 gatttcgtgg cttctgattt gctttctcag gctgaacacg gcccggactc acaagtgatt
    13681 ttactgaccc ccgacgccga tatggcgcgt cgcgttgccg aggccgtcga acgccaactg
    13741 gcagaactgc cgcgtgccga aaccgcccgc caggcactga gcgccagccg cctgatcgtg
    13801 actaaagatt tagcgcagtg cgtagagatt tctaatcagt acggcccgga gcacctgatc
    13861 attcagaccc gcaacgcccg cgagctggtc gatggcatca ccagcgccgg ttcggtattt
    13921 cttggtgact ggtcaccgga atcggcaggc gactatgcct ccggtaccaa ccacgtacta
    13981 ccgacttacg gttataccgc gacgtgttcc agcctcggcc tggctgattt ccagaagcgt
    14041 atgaccgtgc aggagctgtc gaaagaaggt ttctccgcgc tggcttcgac cattgaaaca
    14101 ctgaccgccg ccgagcgcct gactgcccac aaaaatgccg ttactttgcg tgttaacgcc
    14161 cttaaggagc aagcatgaac accgtgacta ttaccgattt agcgcgtgaa aacgtccgca
    14221 acctgacgcc gtatcagtcg gcgcgtcgtc tgggcggtaa cggcgacgtc tggctgaacg
    14281 ccaacgaata ccccacagcc gtggagtttc agctcactca acaaacgctc aaccgctacc
    14341 cggaatgcca gccgaaagcg gtgattgaaa attacgcgca atatgcaggc gtaaaaccgg
    14401 agcaggtgct ggtcagccgt ggcgcggacg aaggtattga actgctgatt cgcgcttttt
    14461 gcgaaccggg taaagacgcc atcctctact gcccgccaac gtacggcatg tacagcgtca
    14521 gcgctgaaac cattggcgtc gagtgccgca cagtgccgac gctggacaac tggcaactgg
    14581 acttacaggg catttccgac aagctggacg gcgtaaaagt ggtctatgtt tgcagcccca
    14641 acaaccccac cgggcaactg atcaatccgc aggattttcg caccctgctg gagttaaccc
    14701 gcggtaaggc gattgtggtt gccgatgaag cttatatcga gttttgcccg caggcatcgt
    14761 tagctggctg gctggcggaa tatccgcacc tggcgatttt gcgtaccctt tcgaaagcct
    14821 tcgcgttggc ggggcttcgt tgcggattta cgctggcaaa cgaagaagtc atcaacctgc
    14881 tgatgaaagt gatcgccccc tacccgctct cgacgccggt tgccgacatt gccgcacagg
    14941 cgttaagccc gcagggaatc gtcgccatgc gcgaacgggt ggcgcaaatt attgcagaac
    15001 gcgaatatct gattgccgca ttgaaagaaa tctcctgcgt ggagcaggtt tttgactctg
    15061 aaaccaacta cattctggcg cgctttaaag cctccagtgc agtgtttaaa tctttgtggg
    15121 atcagggcat tatcttacgt gatcagaata aacaaccctc tttaagcggc tgcctgcgaa
    15181 ttaccgtcgg aacccgtgaa gaaagccagc gcgtcattga cgccttacgt gcggagcaag
    15241 tttgatgagt cagaagtatc tttttatcga tcgcgatgga accctgatta gcgaaccgcc
    15301 gagtgatttt caggtggacc gttttgacaa actcgccttt gaaccgggcg tgatccctca
    15361 gctgctgaag ctgcaaaaag cgggctacaa actggtgatg atcactaatc aggatggtct
    15421 gggaacacaa agtttcccgc aggcggattt tgatggcccg cacaacctga tgatgcagat
    15481 cttcacctcg caaggcgtgc agtttgatga agtgctgatt tgtccgcacc tgcccgccga
    15541 tgagtgcgac tgccgtaagc cgaaagtaaa actggtggag cgttatctgg ctgagcaagc
    15601 gatggatcgc gctaacagtc atgtgattgg cgatcgcgcg accgacattc aactggcgga
    15661 aaacatgggc attaatggtt tacgctacga ccgcgaagcc ctgaactggc cgatgattgg
    15721 tgagcaactc actaaacgag accgttacgc ccatgtagtg cgcaacacca aagagacgca
    15781 gattgacgtc caggtgtggc tggatcgcga aggtggcagc aagattaaca ccggcgttgg
    15841 cttctttgat cacatgctgg atcagatcgc cacccacggc ggtttccgga tggaaatcaa
    15901 cgtcaaaggc gacctctata tcgacgatca ccacaccgtc gaagataccg gcctggcgct
    15961 gggcgaagca ttaaaaattg ctcttggcga caaacgcggt atttgccgct ttggttttgt
    16021 actgccgatg gacgaatgcc ttgcccgctg cgcgctggat atctctggtc gcccgcacct
    16081 ggaatataaa gccgagttta cctaccagcg cgtgggcgat ctcagcaccg aaatgatcga
    16141 gcacttcttc cgttcgctct catacaccat gggcgtgacg ctacacctga aaaccaaagg
    16201 taaaaacgat caccaccgtg ttgagagtct ctttaaagcc tttggtcgca ccctgcgcca
    16261 ggccatccgc gtggaaggcg acaccctgcc ctcgtcgaaa ggagtgctgt aatgaacgtg
    16321 gtgatccttg atactggctg cgccaacctg aactcggtga agtctgccat tgcgcgccac
    16381 ggttatgaac ccaaagtcag ccgtgacccg gacgttgtgt tgctggccga taaactgttt
    16441 ttacccggcg tcggcaccgc acaagcagcg atggatcagg tgcgtgagcg cgagctgttt
    16501 gatctcatca aagcctgtac ccaaccggtg ctgggcatct gcttagggat gcaacttctg
    16561 gggcggcgca gcgaagagag caacggcatc gacttgctgg gcatcatcga cgaagacgtg
    16621 ccgaaaatga ccgactttgg tctgccactg ccgcatatgg gctggaaccg cgtttatccg
    16681 caggcaggca accgcctgtt tcaggggatt gaagacggcg cgtactttta ctttgttcac
    16741 agctacgcaa tgccagtcaa tccgtggacc atcgcccagt gtaattacgg cgaaccgttc
    16801 accgcggcgg tacaaaaaga taacttctac ggcgtgcagt tccacccgga gcgttctggt
    16861 gccgctggcg ctaagttgct gaaaaacttc ctggagatgt aatgattatt ccggcattag
    16921 atttaatcga cggcaccgtg gtgcgtctcc atcagggcga ttacggcaaa caacgcgatt
    16981 acggtaacga cccactgcca cgcttacagg attacgctgc gcaaggtgcc gaagtgttgc
    17041 acctggtgga tctgaccggg gcaaaagatc cggctaagcg tcaaatcccg ctgattaaaa
    17101 ccctggtcgc gggcgttaac gtcccggtgc aggttggcgg cggtgtgcgt agtgaagaag
    17161 acgtggcggc gttactggaa gcgggcgttg cgcgcgtggt ggttggctcc accgcggtga
    17221 aatcaccaga aatggtgaaa ggctggtttg aacgtttcgg tgccgatgcc ttagtgctgg
    17281 cgctggatgt ccgtattgac gagcaaggta acaagcaggt ggcggtgagc ggctggcaag
    17341 agaactcggg cgtttcactg gaacaactgg tggaaaccta tctgcccgtc ggcctgaaac
    17401 atgtgctatg taccgatatc tcgcgcgacg gcacgctggc aggttctaac gtctctttat
    17461 atgaagaagt gtgcgccaga tacccgcagg tggcatttca gtcctccggc ggcattggcg
    17521 acattaacga tgttgctgcc ctgcgaggta ctggcgtgcg cggcgtaata gttggtcggg
    17581 cattactgga aggtaaattc accgtgaagg aggctatcgc atgctggcaa aacgcataat
    17641 tccatgtctc gacgttcgtg atggtcaggt ggtgaaaggc gtacagtttc gcaaccatga
    17701 aatcattggc gatatcgtgc cgctggcaaa acgttacgcc gaagaaggtg cagacgaact
    17761 ggtgttctac gatatcaccg cttccagcga tggccgtgtg gtagataaaa gctgggtatc
    17821 tcgcgtggcg gaagtgatcg acattccgtt ctgtgtagcg ggtgggatta agtctctgga
    17881 agatgccgcg aaaattcttt cctttggcgc ggataaaatt tccatcaact cgcctgcact
    17941 ggcggacccg acgttaatta ctcgcctggc ggatcgcttt ggcgtgcagt gtattgtggt
    18001 cggtattgat acctggtacg acgccgaaac cggtaaatat catgtgaatc aatataccgg
    18061 cgatgaaagc cgcacccgcg tcactcagtg ggaaacgctc gactgggtac aggaagtgca
    18121 aaaacgcggt gccggagaaa tcgtcctcaa tatgatgaat caggacggcg tgcgtaacgg
    18181 ttacgacctc gaacaactga aaaaagtacg tgaagtttgc cacgtcccgc taattgcctc
    18241 cggtggcgcg ggcaccatgg aacacttcct cgaagctttc cgcgatgccg acgttgacgg
    18301 tgcgctggca gcttccgtat tccacaaaca aataatcaat attggtgaat taaaagcgta
    18361 cctggcaaca cagggcgtgg agatcaggat atgttaacag aacaacaacg tcgcgaactg
    18421 gactgggaaa aaaccgacgg actgatgccg gtgattgtac aacacgcggt atctggcgaa
    18481 gtgttaatgc tgggctatat gaacccggaa gccttagaca aaaccatcga aagcggcaaa
    18541 gtcaccttct tctcgcgcac taaacagcga ctgtggacca aaggcgaaac gtcgggcaat
    18601 ttcctcaacg tagtgagtat tgcgccggac tgcgacaacg acactttatt agtgctggcg
    18661 aatcccatcg gcccgacctg ccacaaaggc accagcagct gcttcggcaa caccgctcac
    18721 cagtggctgt tcctgtatca actggaacaa ctgctcgccg agcgcaaatc tgccgacccg
    18781 gaaacctcct ataccgccaa actgtatgcc agcggcacca agcgcattgc gcagaaagta
    18841 ggcgaagaag gcgtggaaac tgcgctggca gcaacggtac atgaccgctt tgagctgacc
    18901 aacgaggcat ctgacttgat gtatcacctg ctggtgttgt tgcaggatca ggggctggat
    18961 ctaggagaag tgattgataa tctgaagaat cggcattaac gcgttggccc ccgcagcaac
    19021 ggcgggggta gcatctctca ggatttgctt tccagcaatg tagtgtagag attgacatag
    19081 gcgtcaatca tatggtcggc agtaaacatc tcttcaaaac gacgacgcga gttttcgcca
    19141 tagcggttgg aggtttcctc gttattccag agctcattca tcgcctctac cagcgcctgg
    19201 ctatcattcg gcggaatcac gcaaccactc actttgtcct ggttaatgaa agaggtacct
    19261 gtaccaatct cgcaagagat cagtggcctt gcgaagcgtg cgccttccaa taacgtaagt
    19321 aagcgtgcag tgagaaccgt attgacgaag tgaagctggt caggcaggca gagtaacccg
    19381 ccacgggagc agagcaccca cccggttcac aggccagtcg gcgatgacat caagtacatg
    19441 atgcagatag cgt
//
LOCUS       DAPHDW010000077        19216 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.077, whole genome shotgun sequence.
ACCESSION   DAPHDW010000077 DAPHDW010000000
VERSION     DAPHDW010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.077"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(122..547)
                     /gene="exbD"
                     /locus_tag="RH057_002782"
     CDS             complement(122..547)
                     /gene="exbD"
                     /locus_tag="RH057_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HDV3129129.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(554..1288)
                     /gene="exbB"
                     /locus_tag="RH057_002783"
     CDS             complement(554..1288)
                     /gene="exbB"
                     /locus_tag="RH057_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HDV3129130.1"
                     /translation="MGNNLMQTDLSVWGMYQHADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            1540..2727
                     /gene="metC"
                     /locus_tag="RH057_002784"
     CDS             1540..2727
                     /gene="metC"
                     /locus_tag="RH057_002784"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HDV3129131.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFDSVEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGV
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNNEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            2867..3526
                     /locus_tag="RH057_002785"
     CDS             2867..3526
                     /locus_tag="RH057_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3129132.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGRWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVISFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(3566..4465)
                     /gene="yqhC"
                     /locus_tag="RH057_002786"
     CDS             complement(3566..4465)
                     /gene="yqhC"
                     /locus_tag="RH057_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HDV3129133.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            4659..5822
                     /gene="yqhD"
                     /locus_tag="RH057_002787"
     CDS             4659..5822
                     /gene="yqhD"
                     /locus_tag="RH057_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HDV3129134.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGAGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            5927..6754
                     /gene="dkgA"
                     /locus_tag="RH057_002788"
     CDS             5927..6754
                     /gene="dkgA"
                     /locus_tag="RH057_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HDV3129135.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGEIAKLDQGKRLGPDPDQFGG"
     gene            6954..7880
                     /locus_tag="RH057_002789"
     CDS             6954..7880
                     /locus_tag="RH057_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HDV3129136.1"
                     /translation="MKIILLFLAALASFTVHAQPPSLTVEQTVRHIYQNYKSDATAPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     ADHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPEAFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            7931..8188
                     /gene="yqhH"
                     /locus_tag="RH057_002790"
     CDS             7931..8188
                     /gene="yqhH"
                     /locus_tag="RH057_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HDV3129137.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(8231..10450)
                     /locus_tag="RH057_002791"
     CDS             complement(8231..10450)
                     /locus_tag="RH057_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HDV3129138.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTPGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VTVQPPRPKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKNPLAKIGYK
                     SEDVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(10561..11973)
                     /gene="ftsP"
                     /locus_tag="RH057_002792"
     CDS             complement(10561..11973)
                     /gene="ftsP"
                     /locus_tag="RH057_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HDV3129139.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(12048..12785)
                     /gene="plsC"
                     /locus_tag="RH057_002793"
                     /gene_synonym="parF"
     CDS             complement(12048..12785)
                     /gene="plsC"
                     /locus_tag="RH057_002793"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HDV3129140.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(13019..15277)
                     /gene="parC"
                     /locus_tag="RH057_002794"
     CDS             complement(13019..15277)
                     /gene="parC"
                     /locus_tag="RH057_002794"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HDV3129141.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDSACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(15823..16305)
                     /locus_tag="RH057_002795"
     CDS             complement(15823..16305)
                     /locus_tag="RH057_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3129142.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDDPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(16358..16750)
                     /gene="ygiW"
                     /locus_tag="RH057_002796"
     CDS             complement(16358..16750)
                     /gene="ygiW"
                     /locus_tag="RH057_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HDV3129143.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSATQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            16902..17561
                     /gene="qseB"
                     /locus_tag="RH057_002797"
     CDS             16902..17561
                     /gene="qseB"
                     /locus_tag="RH057_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HDV3129144.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLILTARDALEERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            17558..18907
                     /gene="qseC"
                     /locus_tag="RH057_002798"
     CDS             17558..18907
                     /gene="qseC"
                     /locus_tag="RH057_002798"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_306641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HDV3129145.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGDKDQWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHYGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNAVRYSPQGSVIDVTLNADNFIVRDNGPGV
                     TPEALARIGERFYRPPGQTATGSGLGLSIVQRIAKLHGMNVEFGNAEQGGFEAKVSW"
     gene            complement(18953..>19216)
                     /locus_tag="RH057_002799"
     CDS             complement(18953..>19216)
                     /locus_tag="RH057_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HDV3129146.1"
                     /translation="DAFFAFNKYDWMLESGDNICSIPSVSGEDRILQAMIAAFFLLTP
                     LIILILRKLFMREMFEFWVYVFSLGICLVCGWWLFWGRFIFCY"
BASE COUNT         4711 a         5093 c         4870 g         4542 t
ORIGIN      
        1 ttgagcacct aatgccggat gcggcgtgaa cgccttatcc ggcctacaaa cgcacgaaga
       61 ttcaacatgt tgcaatagat attgtaggcc tgatgagcgt agcgcatcag gcaattctac
      121 tttacttcgc tttggcggtt tcttcgccga ccagacctat cttcaggtaa cccgcctgat
      181 gcagcgtatc cattaccttc atcaacgtct cgtaatcgac ggttttatcc gctcggaaga
      241 agatggtggt gtctttcttg ccttcggtta acgcattcaa cgccgtaatc attgtttcat
      301 cggtgaccgg atcgttaccg ataaacatcg agttgtctgc cttcaccgac agataaaccg
      361 gtttttccgg gcgcggctgc ggcgtgctgg tagaagcagg caagttcacc ttcacatcta
      421 ccgtcgctaa cggtgccgcc accataaaga taatcagcag aaccaacatc acgtcgataa
      481 acggcgtcac gttgatatca tgcatttcgc cgttatcgtc gaggttttcg ttaagatgca
      541 ttgccatcgg atattatcct gcgcgtaatt tttgtgcgac acgaaccgga tgcgcagcgg
      601 cgctggcttc cagatccagg tcacggcttt gcagcaacaa tacctgcgct gcaacatcac
      661 ccagcatcgc tttaaagccg ccaatctggc gtgcaaatac gttatagata acgaccgcag
      721 gaatcgctgc cacgaggccg attgccgttg ctaacagagc ttctgcgata cccggcgcaa
      781 cgactgccag gttagtggtc tgcgtttgcg cgataccaat aaagctgttc atgatgcccc
      841 agaccgtacc aaacagacca acgaacggag aaatcgcgcc gatggttgcc agatagccgt
      901 taccgcggcc catttgacga cccactgcgg cgacccgacg ctccagacgg aagctggtac
      961 gttctttaat accttcgtta tcgtcgctgc cttctgacag ttccagctca ttctgcgctt
     1021 cattgagcaa atgcaggctt aagcttttgc taccaaaatc agcggcgata tcgttggcct
     1081 ggtttaagga acgcgcttca gccagcagtt gctgctcgcg cttaaggcga cgcttctgat
     1141 taaagaactc tacgctctta ctgaagaaga ttgcccaggt gactacggag gccaaaataa
     1201 gcccaatcat cacgcactta acgacaatat cggcgtgctg atacataccc cagacggaaa
     1261 ggtccgtctg cattaaatta ttacccactg tgtatctcca ggacgcaagt cacaaaatct
     1321 gcgcataata atatcaaaac gacgtcgaat tgatagtcgt tctcattact atttgcatac
     1381 tgccgtacct ttgctttctt ttccttgcgt ttacgcagta aaaaagtcac cagcacgcca
     1441 tttgcgaaaa ttttctgctt tatgccaatt cttcaggatg cgcccgcgaa tattcatgct
     1501 agtttagaca tccagacgta taaaaacagg aatcccgaga tggcggacaa aaagcttgat
     1561 actcaactgg tgaatgcagg acgcagcaaa aaatacactc tcggcgcggt aaatagcgtg
     1621 attcagcgcg cttcttcgct ggtctttgac agtgtggaag ccaaaaagca cgcgacgcgc
     1681 aatcgcgcca atggcgagtt gttctatgga cggcgcggaa cgttaaccca tttctcctta
     1741 caacaagcga tgtgtgaact ggaaggtggc gcaggctgcg cgctatttcc ctgcggggcg
     1801 gcagcggttg ctaattccat tcttgctttt gtcgaacagg gcgttcatgt gttgatgacc
     1861 aacaccgcct atgaaccgag tcaggatttc tgtagcaaaa tcctcagcaa actgggcgta
     1921 acgacatcgt ggtttgatcc gctgattggt gccgatatcg ttaagcatct gcagccaaac
     1981 actaaaatcg tgtttctgga atcgccaggc tccatcacca tggaagtcca cgacgttccg
     2041 gcgattgttg ccgccgtacg cagtgtggtg ccggatgcca tcattatgat cgacaacacc
     2101 tgggcagccg gtgtgctttt taaggcgctg gattttggca tcgatgtttc tattcaagcc
     2161 gccaccaaat atctggttgg gcattcagat gcgatgattg gcactgccgt gtgcaatgcc
     2221 cgttgctggg agcagctacg ggaaaacgcc tatctgatgg gccagatggt cgatgccgat
     2281 accgcctata taaccagccg tggcctgcgc acattaggtg tgcgtttgcg tcaacatcat
     2341 gaaagcagtc tgaaagtggc tgaatggctg gcagaacatc cgcaagttgc gcgagttaac
     2401 caccctgctc tgcctggcag taaaggccac gaattctgga aacgagactt tacaggcagc
     2461 agcgggctat tttcctttgt gcttaagaaa aaactcaata atgaagagct ggcgaactat
     2521 ctggataact tcagtttatt cagcatggcc tactcgtggg gcgggtatga atcgttgatc
     2581 ctggcgaatc aaccagaaca tatcgccgcc attcgccccc aaggcgagat cgattttagc
     2641 gggaccttga ttcgcctgca tattggtctg gaagatgtcg acgatctgat tgccgatctg
     2701 gacgccggtt ttgcgcgaat tgtataacat tgccactttt ggacaatttt gcagacattt
     2761 aattgtgaaa agtcttaaat tgttgcgtcc gggatcaagg cgtcccggac gaatcaggag
     2821 tacaataggc agataaaggc ttaaacgctg ttccacagga aagtccatgg ctgttattca
     2881 agatatcatc gctgcgctct ggcaacacga ctttgccgcg ctggcggatc ctcatattgt
     2941 tagcgttgtt tactttgtca tgtttgccac gctgttttta gaaaacggcc tgctgcccgc
     3001 ctcatttttg ccaggcgaca gcttgttgat actggcaggc gcattgattg cccaaggggt
     3061 tatggatttt ctgcctacga ttgcgattct gaccgccgca gcaagtctgg gctgctggct
     3121 aagttatatt caggggcgct ggttagggaa taccaaaacg gtgaaaggct ggctggcaca
     3181 gcttcctgct aaatatcacc agcgcgccac ctgcatgttt gaccgccacg gtctgctggc
     3241 gctgctggct ggacgttttc ttgcatttgt ccgtacgctg ctgccaacca tggcgggaat
     3301 ttccggtctg ccaaaccgcc gcttccagtt tttcaactgg ttaagtggat tgctgtgggt
     3361 cagcgtggta atcagttttg gctatgcctt aagtatgatt ccgttcgtta aacgccatga
     3421 agatcaggta atgacgttcc tgatgatcct gccaattgcc ttgttaaccg ctggcttgtt
     3481 aggcacgctg tttgtggtga ttaaaaaaaa atactgtaac gcctgacgat tttccccgtt
     3541 cccggctgct gtaccgggaa cgtatttaat tcccctgcat cgcccgcatt cttgccgcat
     3601 cttcccccgg cgtcacaccg aagtaacgtt taaactcacg gctaaattgc gatgcgcttt
     3661 catagccgac gcgcatcgct gctgcgctgg ccttcatgcc gtcatggatg atcatcatcc
     3721 gcgccttatg cagacggtaa ttcttcaaat actgcaacgg cgaggtgctg gtgacagact
     3781 taaaattatg gtggaacgcc gatacgctca tgttggcttc tgccgccagt tgctcgacgc
     3841 tcaggttttc ggtgtattta ttctcaatcc gtttcagcac gcggctaatc agactgaagt
     3901 gagtctggcg actgaccagc gccagtaacg cgccgccgca aggtccggtc agcacgtagt
     3961 acagaatttc gcggatgatc tgtttgccga gaatacgcgc atccagtggt cgctccatca
     4021 cgtcgagtaa ccgctccgcc gcgcataaaa tctcttctga taacgtggcg gagttaatcc
     4081 cgctggctgc catcgacggc tggaaatgct catcttcgcc aatgtccatc aacagttcct
     4141 gtaactgcaa aatatcgaca ttgagacgca accctgccag cggcacctct gacgtggcat
     4201 aggtttcgca ctcaaacggc aacggcaccg tcagcagcag gtattcattg gcatcataac
     4261 gaaacacgcg ttcattgata taaccgattt tatgcccgga aaagagaatt atgatgccag
     4321 gctcgtacat caccggtgta cgtgcgaaag gcgtctcgcc atacaacaaa cgcacatcgg
     4381 gcaacagttc tgacaaacta ttttctttat ttttcagttt attaacttta tccgccagca
     4441 agcggcaaat ctcttcacgt ttcatatcgc gtaatttctt aggaataatg cggcaatttg
     4501 attgtgcgca attttgtagc atttctccag cactctggag aaataggcaa gacattggca
     4561 gaaatgagca ttgagagcca gggcgctggc gatcacaatg aaaaacatca ggcagatcgt
     4621 tctctgccct catattggcc cagcaaaggg agcaagtaat gaacaacttt aatctgcaca
     4681 ccccaacccg cattctgttt ggtaaaggcg caatcgctgg tttacgcgaa caaattcctc
     4741 acgatgctcg cgtattgatt acctacggtg gcggcagcgt gaaaaaaacc ggcgttctcg
     4801 atcaagttct ggatgccctg aaaggcatgg acgtgctgga atttggcggt attgagccaa
     4861 acccggctta tgaaacgctg atgaacgccg tgaaactggt tcgcgaacag aaagtgactt
     4921 tcctgctggc ggttggcggc ggttctgtac tggacggcac caaatttatc gccgcagcgg
     4981 ctaactatcc ggaaaatatc gatccgtggc acattctgca aacgggcggt aaagagatta
     5041 aaagcgccat cccgatgggc tgtgtgctga cgctgccagc aaccggttca gaatccaacg
     5101 caggcgcggt gatctcccgt aaaaccacag gcgacaagca ggcgttccat tctgcccatg
     5161 ttcagccggt atttgccgtg ctcgatccgg tttataccta caccctgccg ccgcgtcagg
     5221 tggctaacgg cgtagtggac gcctttgtac acaccgtgga acagtatgtt accaaaccgg
     5281 ttgatgccaa aattcaggac cgtttcgcag aaggcatttt gctgacgcta atcgaagatg
     5341 gtccgaaagc cctgaaagag ccagaaaact acgatgtgcg cgccaacgtc atgtgggcgg
     5401 cgactcaggc gctgaacggt ttgattggcg ctggcgtacc gcaggactgg gcaacgcata
     5461 tgctgggcca cgaactgact gcgatgcacg gtctggatca cgcgcaaaca ctggctatcg
     5521 tcctgcctgc actgtggaat gaaaaacgcg agaccaagcg cgctaagctg ctgcaatatg
     5581 ctgaacgcgt ctggaacatc actgaaggtt ccgacgatga gcgtattgac gccgcgattg
     5641 ccgcaacccg caatttcttt gagcaattag gcgtgccgac ccacctctcc gactacggtc
     5701 tggacggcag ctccatcccg gctttgctga aaaaactgga agagcacggc atgacccaac
     5761 tgggcgaaaa tcatgacatt acgctggatg tcagccgccg tatatacgaa gccgcccgct
     5821 aagcttttta cgcctcaaac tttcgttttc gggcatttcg tccagactta agttcacaac
     5881 acctcaccgg agcctgctcc ggtgagttca tataaaggag gaacgtatgg ctaatccaac
     5941 cgttattaag ctacaggatg gcaatgtcat gccccagctg ggactgggcg tctggcaagc
     6001 aagtaatgag gaagtaatca ccgccattca aaaagcgtta gaagtgggtt atcgctcgat
     6061 tgataccgcc gcggcctaca agaacgaaga aggtgtcggc aaagccctga aaaatgcctc
     6121 agtcaacaga gaagaactgt tcatcaccac taagctgtgg aacgacgacc acaagcgccc
     6181 ccgcgaagcc ctgctcgaca gcctgaaaaa actccagctt gattatatcg acctctactt
     6241 aatgcactgg cccgttcccg ctatcgacca ttatgtcgaa gcatggaaag gcatgatcga
     6301 attgcaaaaa gagggattaa tcaaaagcat cggcgtgtgc aacttccaga tccatcacct
     6361 gcaacgcctg attgatgaaa ctggcgtgac gcctgtgata aaccagatcg aacttcatcc
     6421 gctgatgcaa caacgccagc tacacgcctg gaacgcgaca cacaaaatcc agaccgaatc
     6481 ctggagccca ttagcgcaag gagggaaagg cgttttcgat cagaaagtca ttcgcgatct
     6541 ggcagataaa tacggcaaaa ccccggcgca gattgttatc cgctggcatc tggatagcgg
     6601 cctggtggtg atcccgaaat cggtcacacc ttcacgtatt gccgaaaact ttgatgtctg
     6661 ggatttccgt ctcgacaaag acgaactcgg cgaaattgca aaactcgatc agggcaagcg
     6721 tctcggtccc gatcctgacc agttcggcgg ctaacatgca aattctcccg gtggcggtaa
     6781 tgttccgcta ccggactttt cagaaatcat ttattcccct cgcgtcccgc ccgttgttac
     6841 tcttccttgt tcaggaatgc caaatataag gacatcatca tgcagagccg gaagctctta
     6901 aaagaacaac tcatctatat ccgggataaa cgcaacggag aggtgaaaaa cagatgaaaa
     6961 taatacttct gtttttagca gccctggcaa gttttaccgt acacgcacag cccccctcac
     7021 tgaccgtaga acaaacagtc cggcatattt atcagaacta taaatcagat gccactgccc
     7081 cttattttgg tgaaaccgga gagcgggcga taacttctgc gcgtattcaa caggcgctta
     7141 ccctgaacga caatcttacg ctgccgggca atattggctg gctggattat gatccggttt
     7201 gtgattgtca ggattttggc gatctggtgc tagaaagcgt tgcgataacc caaactgacg
     7261 ccgatcatgc cgatgccgtt gtgcgctttc gtatctttaa agatgataaa gaaaagacca
     7321 cgcagacact gaaaatggtg gcggaaaatg gtcgttgggt cattgacgat attgtcagca
     7381 atcatggcag cgtcttacaa gcagttaata gcgagaatga aaaaacgctg gccgctttag
     7441 cttcgttgca aaaagaacag ccggaagcct ttgttgccga actctttgaa catattgctg
     7501 attatagctg gccgtggacg tgggtggttt ccgactctta ccgccaggcg gttaatgcct
     7561 tctataaaac caccttcaag acggccaata atcccgatga agatatgcaa atagaacggc
     7621 aatttattta cgacaatccg atctgttttg gcgaagagtc gctattttca cgcgttgatg
     7681 aaattcgagt cctggagaaa accgccgatt ccgcccgcat tcatgttcgt tttacgctga
     7741 ccaatggcaa caacgaagag caagaactgg ttttacagcg gcgcgaaggc aagtgggaaa
     7801 tcgctgattt tatccgcccg aacagcggca gcctacttaa gcagattgag gcaaaaaccg
     7861 ccgccagatt aaagcaatga gctgaattaa ataacaatta gccggaacaa taaataaaag
     7921 ggaacactat atgaaaacga ttttcaccgt gggagctgtt gttctggcaa cctgcttgct
     7981 cagtggctgc gtcaatgagc aaaaggtcaa tcagctggcg agcaatgtgc aaacattaaa
     8041 tgccaaaatc gcccggcttg agcaggatat gaaagcacta cgcccacaaa tctatgctgc
     8101 caaatccgaa gctaacagag ccaatacgcg tcttgatgct caggactatt ttgattgcct
     8161 gcgctgcttg cgtatgtacg cagaatgata aaaaaatccc cggcagcatg tcagttgccg
     8221 gggatttttt ttaacgtcca accgccgctt tagggcgttt cttcgcacca gcattcaccg
     8281 gacgagattg cgtagacgac gctttttttg ccgtagcagg cgtctgacgc tgggtcgcca
     8341 tcggcgtatg tttcgtcaac gccggacggg tattgcggtt ctggcgacga gcttcacgca
     8401 tctcttcaat ggttggcgca ggcactaagc aatcgcgacg gctgccaatc agatgctttt
     8461 tgcccatcgc ttccagcgcc tggcggatta acggccagtt tgccggatcg tggtaacgca
     8521 acaacgcttt atgcaaacga cgctgtttgt cgcccttcgg tacgaagacg tcttcgctct
     8581 tataaccaat cttcgccagc gggtttttcc cggtgtaata catggtggtt gagttcgcca
     8641 gcggcgatgg gtagaagttc tgtacctggt cgagacggaa acgatgcttt ttcagccaca
     8701 gcgccagatt caccatatct tcatcacgcg taccggggtg cgcggagatg aaatagggga
     8761 tcagatactg ctctttacct gcctgttttg agtaagtatc gaacagctct ttaaagcggt
     8821 catagctgcc catgcccggc ttcatcatct tcgataacgg cccttcttcg gtatgttccg
     8881 gggcaatctt cagataaccg ccgacgtgat gggtcgccaa ctctttgata tagcgcggat
     8941 cttccacggc gatgtcataa cgtacgcctg aggcgatgag gattttttta atgcctttca
     9001 gatcacgcgc acggcgatag aggttgatcg tcggttcgtg gttagtgtcc atgtgcggac
     9061 aaatatccgg ataaacgcac gacaaacggc gacaagtttg ttcagcgcgt ggcgatttgc
     9121 agcgcaacat atacatgttg gcagttggcc caccgagatc ggaaatcacg cccgtaaaac
     9181 ctggaacggt gtcgcggatc gcttcgatct cattaatgat cgaatcttcc gaacggctct
     9241 gaataatgcg cccttcgtgc tcggtaatag aacagaaaga acagccgcca aagcagccgc
     9301 gcataatgtt gaccgaaaaa cggatcattt cgtaagccgg aatacgggca ttgccatagg
     9361 ccggatgtgg cacgcgcttg tacggcagcg caaaaacgct gtccatctct tcggtagaaa
     9421 gcgggatagc aggcgggttg atccacacat agcggtcacc gtgtttttgc atcaatgcgc
     9481 gggcacagcc tgggttggtt tcgtggtgca gaatgcgcga agcatgggcg tacagcactt
     9541 tatcgccctt cactttctcg aaagaaggca gcaacacgta ggttttttcc cacggtttcg
     9601 ggcgcggtgg ctgcacggtt acggctttgg cttcctgctt tttcggtgcc accggtttgt
     9661 tatccgcgca cggcaaatct tcaccatacg gatgcgggat tgggtcgatt tttccagggg
     9721 tatcaagacg ggtggaatcc acgccgctcc agccaggcag cgcctctttc acgataatcg
     9781 cggtattacg cacatcgcgg atttcactaa tcggctcgcc catcgccaga cggtgcgcca
     9841 cctccaccag cggacgctca ccgttaccaa acatcagcat gtcggctttc gaatccacca
     9901 gcacggaacg gcgcacggta tcggaccagt aatcataatg tgcggtacgg cgcagactag
     9961 cctcaatacc gccgaggatc accggtacat ctttccacgc ctctttacaa cgctgggtat
    10021 aaaccagtgt ggcgcgatcc gggcgcttac ccgcgacgtt atccggcgtg taggcatcgt
    10081 catgacgtaa acggcgatcg gcggtataac ggttgatcat cgaatccatg ttgccagcag
    10141 taacaccgaa aaacagattc ggtttaccca gacgcatgaa atcgtctttg ctgctccagt
    10201 ctggctgggc gatgatcccg acgcgaaagc cctgtgcttc cagcatacga ccgcaaatcg
    10261 ccatcccgaa gcttgggtga tcgacatacg cgtcgccagt aaccaaaatg atgtcgcagc
    10321 tatcccagcc aagttgatcc atctcttcac gagacatcgg caaaaacggt gccggtccaa
    10381 aacaggcggc ccagtactgc ggccaggaga acaggtcgcg atccggttgg atcagggaga
    10441 tagagctcat tttgcttcca gaaatgataa aaaaataatc aaaggccggg gattataagc
    10501 cggaacgaaa gagaaatcga aaggtattcc atactcgccc tcctcgggcg agtatgaaga
    10561 ttacggtacc ggattgacca aaagttgccc tatcgagcca cggtccgcca tttccagcgt
    10621 ctgactgttg aagtagaacg ggaagtgcgc ccaggaaggc tgaccgaaat aaacaagcag
    10681 ctccacttgt ccgtcaaccc aaacggtatc tttccagcct ctgtcttccg ggaacggcat
    10741 cgcgccattc acgttacgga tctggaacat tacgccttca atatggaacg cttgcggctc
    10801 gtccgcgcgt accgtccagc gttcccacgt tccttgctgc gcggtgacat caatacggtt
    10861 gacgtcccac agttgtccat taatacccgg gtcatcaccc agactgatat cgcgactgcg
    10921 aatcggcgaa ccggccatga tttcagttgg cagcaagcgc atcggaagac tgtctgtgac
    10981 cagcggcaga aggccggttg ggcgcagcgt tagcaccagg gtagaaacca gaatgctgga
    11041 tggctcaaag aagccacgaa tacgatcaac aatgctcgcc gcttcgccac aggtgatcga
    11101 cacttcatcg ccgttgctca tatccaccag aatctcgcgg cgctcgcccg gtgccagcga
    11161 aagttgcttc accgatacag gagcaggcag gaatccctga tcgccagaaa tcacatgtaa
    11221 cgggcgacca tcgctcatct gtagttgata acgacgagag ttcgacgcgt tcagcaggcg
    11281 caagcgcacc cagccacgcg agacttcaac gtacgggctt tgtacaccgt taaccagcag
    11341 cgtatcacca acaaagccgc cgcttcccgg ttcgttgtat tctggcgtac caaagttatc
    11401 cagccgttta tcctgaataa tgaccggaaa atcatccaca ccataatggt tggggatagg
    11461 cagcgacttg ctgacttcat cttccaccag ccacattccg gcaaggccgt tatagacctg
    11521 ctgagccgtg cggttgggag tattggcgtg ataccacaga gtagctgcgt tctggcgaat
    11581 aggcagtacg ggtgcccagt cagcgtttgg cgacatcatc cgtgccggac cgcccatcag
    11641 cgggcctggt acctgtagcc cggctaccgt cattgagaca ttttctgtca ggcggttgct
    11701 gtaaataagc ttaacatcgt cgcccttcca gacgcggata gtcggcccca ggtaacgacc
    11761 attgattccc cagaccgacg cgcgtgtccc tggcgtaaat gaccagtgcg cacgttgtac
    11821 agtcataaac agcggttgcc cacggcgaga ttcaagcagc ggcggaacgg gtagcggttg
    11881 ctgttgcccg gctgcgctgg ccttcagggg aacagcgcct gcacaaagtg caatccccga
    11941 tgcctgaatg aactgacgcc gactgagtga catatttgct ccatgtaaaa ctggctaaag
    12001 taataccgtg caggaaagtg ttccccgcaa aaatacagat tgccgactta aacttttccg
    12061 gcggcttcgc gttctgcgac ttctttatcg agctcggcga ttttttgttc cattatcgaa
    12121 cgacaatggg cagccagctc acgaacctga tctttgccat actgactgac gtcaattggc
    12181 ggcagcattt cgacaatcac cagaccgttg tgcagtcgat taagattaat cttattcgaa
    12241 gttgtagaga cgcacacggg aataatcggg acgcccgccg caattgccgc gtgaaatgct
    12301 ccagtcttga acggtagcag gccgcgacca cggctgcggg ttccttccgg gaacatccag
    12361 atggaaatac ggcgtttttt gaaatgattc acgacttccg caatggtgcc gtgagcttta
    12421 gtgcgattgt ttctgtcgat caataagttg ccggttaacc agtacaactg cccgaagaag
    12481 gggatccaca gcaagctctt tttacctacc gtcaccgtcg gcggttgcac aatgttcgat
    12541 gctgtcacca tgtcatagtt gttctggtgg ttagcgatat agatagcatt gccgtagctt
    12601 tcagcatctg caggtttgcg gcactcaact ttcaggccga acagcggcgc aagacggcca
    12661 aacatatgcc caaaggtggc cacatgtttc gggttacgcg ggctgaaaag gcagtaaatg
    12721 gagccgaata cacagactaa gatgctgtaa atcacggtaa taataagacg aaagatatat
    12781 agcataacac cctctgaagg tctgagagac tggcattgta cgtcacctga aatcaggtta
    12841 gggcattgtt aaaagccgct ctctggaaaa gcgtattgtt aatcgcaacg catgaataaa
    12901 caacggtttc aagggaattt ttatcgtttc cgggggttga atacgctgcc ggatgacgac
    12961 ttaacgtttc atccggcgtt ccttgcaagc gggaggaaac agcgccctcc ccggcatatt
    13021 actcttcgct atcaccgctg ctggcacggc gaggagagtc gatctcaaca cgatcgatac
    13081 gctgcaaacc gcgcatcaac gtaccgcggc gtccacgttc gccagtgact ttctgtagct
    13141 cttccggacg cagtttaatt ttgcgtttcc caacatgaat ggtcagcgtg ctttgcggcg
    13201 gcagaacgta caattgcgcc agaccatctt ctccacgcgc ggcttctgcc gatggaatgt
    13261 tgataatctt gttgcctttg cccttcgaca gctgcggcag atcgcttacc gggaacatca
    13321 acatacggcc tgcctgagtg attgccagca gcatatcgga agcatcttca atcaccaccg
    13381 gcggcataac atgggcattt tccggtaagg tgatcaaagc cttacctgca cggttacgcg
    13441 ccaccagatc gttaaaggtg cagacgaaac cgtaacccgc atcggaagcc atcagcagtt
    13501 tctgatcgtc gctttccatc agcatatggt caacggtcgc cccaggcggc aacgttaatt
    13561 tgccggtgag cggctcgccc tgaccacgcg ccgacggcag cgtaatcggg tcaatggcat
    13621 agctacgacc ggtggaatca acaaacacta ccggttggtt gctcttacct ttcaccgccg
    13681 ctttgaagct atcacccgct ttataattca ggcccggcgc gtcgatatca tggcctttag
    13741 cgctgcgtac ccagcccatc tgcgacagca caatggtgac aggttcagac ggcagcatgt
    13801 cgtgctcgct catcgctttc gcttcttcgc gttcctgcaa cggcgaacga cgatcgtcac
    13861 cgtaggcttg cgcgtctgcc tgcagttctt tcttcagcag gttattcatt ttacgctcgg
    13921 aagccaaaat gccctgcaac tggtcgcgct ctttttccag ctcactctgc tcaccgcgaa
    13981 tcttcatctc ttccagtttg gcaagatgac gcagtttcag ttcgaggatc gcttccgcct
    14041 gggtttccgt aaggccaaac cgcgacatca gcgccggttt cggttcatct tcgttacgaa
    14101 tgatctcaat cacttcgtcg atgttgagaa acgccaccag caaaccttcc aggatatgca
    14161 ggcgcttgag gactttctcc agacgatagt tcagtcggcg gcgcacggta tcgcgacgga
    14221 acaccagcca ttcggagagg atttccagca ggtttttcac cgccggacga ccatccagac
    14281 cgatcatatt gaggttaatg cgatagctct tttccagatc ggtggtagcg aagaggtggt
    14341 tcatcacctg atccatatcc acgcggttgg aacgcggcac aatcaccaga cgggtcgggt
    14401 tctcgtggtc agattcatcg cgcagatcgt caaccatcgg cagctttttg ttgcgcattt
    14461 gcgcagcaat ttgctccagt acgcgcgcac ctgaaacctg atgcggtaat gcgctgataa
    14521 ccaccgcgcc atcttctttc ttccacaccg cgcgcatacg cactgaacca cgtccgttct
    14581 cgtagatttt acggatctcg gcgcgcgaag tgataatttc cgcttcagtc ggataatccg
    14641 gcccctgcac gatatccagc agctgatcga gcgtggtttt cggctggtcg attaatgcga
    14701 ttgccgcctg agctacttca cgcaggttat gcggtggaat atcggtcgcc atgccgacgg
    14761 caataccggt ggtgccgtta agcaaaatgt ttggcagacg agcaggcagc attttcggct
    14821 cctgcaaagt gccgtcgaag tttggcaccc agtcagccgt cccctgcccc agctcgctca
    14881 atagcagctc ggaatatttc gacaaccggg attcggtgta acgcattgcc gcgaacgatt
    14941 tcggatcgtc cggcgcgccc cagttcccct gaccatcaac cagcggataa cggtaagaga
    15001 acggctgcgc catcaggacc atcgcttcat aacaggcgct atcgccgtgc ggatggtatt
    15061 tacccagtac gtcaccgacg gtacgggccg attttttaaa tttggcgctg gcattcaggc
    15121 ccagttcaga catcgcatac acaatgcggc gctgaacagg tttcagacca tcaccaataa
    15181 acggcaacgc acggtccatg atcacgtaca tggagtagtt taagtaggcg ttttccgtaa
    15241 attcatgtag cgcaaggcgc tctgccatat cgctcattaa ttctgattcc tcaacttatt
    15301 cgccagcctt caaaggggca atattgccgg agatactaca ttatctgccg cgtcgagtca
    15361 caggagatca gcgtgaatag cgccgccgga cgtgctccga cggcgataaa ggcatcaatg
    15421 tgccttgata tacaactctt tgctgtagac ttgatcggca tagagggcag cacaaggttg
    15481 cgcccatgcc acaccactcg gcatgggggt ccgctccggg cgattcgaga agttgaggtt
    15541 atgcgagtcc agggaagcct aaagcatcaa agtagtcgac tgtaagtata cggttcagcc
    15601 cagtttagct gtgtctccac cagtgccccg cgcctcctgc cataagtgcg gcttgctcat
    15661 ggataccccc gagacggctt acggcgcacc ggataataaa tttcgcgtat acatggtatt
    15721 ccttaccgga gaaattataa gcccaacaac aatacgcctg tggcattgtt cgctcaaact
    15781 tatcgcgagt gatttggctc acactccggt aaagattccc catcacgcca acggcacata
    15841 aacatccgtt tccagtcgct gctctgtcac cccttcggca agattggtgt agtgaaacag
    15901 aatcggtgct ttacgcattt tctcgccgct tgcaggcagc cagtggcgaa taatgcccca
    15961 tatcgtgtgt gaaatatcgt ccagctcgcc aacgtggcgg gccacggcat aacgtccacc
    16021 ggtaagttca ccattgctca caccataacg attatcggga atcggttcgc taacgctgcc
    16081 gcagatatca aagcgaaacg cttccggtgc ggtggtggca gggtcgtccc aggcgacgcc
    16141 aaaagtctgg ctttggttaa caggagaaag ccccgtctct ttacgccaca tgataaattt
    16201 cgccacactg tagttgagca actcagggct acagcggtgc ggcaacatcg ccacagggat
    16261 tgatgggaaa tcgataatgt ttacatccag tgtcaggttt gtcattttgc ctccctgcga
    16321 aattattccc gggaacgcgt cccgggagcg gtaacaatta cggatttact ttgcggatct
    16381 gtttgacgtc aatttcaaca gaattccagt ctttatcgac ttcaccctga atctcaaccg
    16441 tatctttcgg cgtcaccgtc acaccgttcc agcgtttgtg gtcgatatca acattgatag
    16501 taccgctggc atctttaaac acgtagagat cgtcagagat gcgttcaacg atattgccgc
    16561 gcagggttac ccaggtgtcg tcacgcaggg attttgcgct ttctacggtc gttacgctgc
    16621 cgttcggccc ctggaatcct ccggcctgac tttgcgttgc cgatgggcca gaaaaaccgc
    16681 cctgctctgc tgccatcacc ggtgcgctgc acagggccat tactgcgatt actgctgcga
    16741 attttttcat gtttattact ccctttaatg tctgtttccg agcatttaac aagatagtcc
    16801 ttaacaactt cttaagggaa aaaaataaaa tttagtgctg tacagagcgc gttacaacac
    16861 ggtttactgg cagcaaatac ggttatcgca gggatgaaaa aatgcgaatt ttactgatag
    16921 aagatgacat gctgattggc gacggcatca aaacgggcct tagtaaaatg ggttttagcg
    16981 tcgactggtt tacacaaggt cgtcagggaa aagaggcgct ttatagcgcg ccttatgatg
    17041 cggtgatcct ggatttaacc ttaccaggca tggatggtcg cgatattttg cgcgaatggc
    17101 gagaaaaagg tcagcgtgag ccggtactga tcctgaccgc gcgcgatgcg ctggaggaac
    17161 gtgtagaagg gctgcgtctg ggagctgacg attatctgtg taaacctttt gcgttgatag
    17221 aagtcgccgc caggctggaa gctctgatgc gccgaaccaa cggccaggcc agcaacgagc
    17281 tgcgccacgg taacgtcatg ctcgaccccg gcaaacgtat cgccacgctg gctggcgaac
    17341 ccttaacact gaaaccaaaa gaatttgccc tgctggaatt actgatgcgt aacgctggtc
    17401 gggtactgcc gcgcaaactg attgaagaga aactgtatac ctgggacgaa gaggtcacca
    17461 gtaatgccgt tgaagtgcat gtgcatcatc tgcgacgcaa actcggtagt gattttattc
    17521 gtaccgtgca tggtattggc tacacattag gtgagaaatg aaatttaccc aacgtcttag
    17581 tctgcgcgtc aggctgacgc taatcttttt aattctggcc tcggtgacct ggctgctttc
    17641 cagctttgtc gcctggaaac aaacaacgga taacgtcgat gaattgttcg acacccaact
    17701 gatgctgttt gccaagcggt taagtacgct cgatctcaac gaaatcaacg cggcggatcg
    17761 catggcacag acgccaaata aattaaaaca cggtcatgtt gatgacgatg cgctgacctt
    17821 tgccatcttt acccacgacg gcagaatggt ccttaacgat ggcgataacg gagaagatat
    17881 tccttatagc tatcaacggg aaggttttgc tgacgggcaa ctggtgggcg ataaagatca
    17941 atggcgtttt gtctggatga cctcacctga tggcaaatat cgcatcgttg ttggtcagga
    18001 atgggaatac cgtgaagaca tggcgctggc gattgttgcc gggcaattga tcccgtggct
    18061 ggtcgcactg ccgattatgt taatcatcat gatggtacta ttgggtcgtg aactcgcgcc
    18121 gctgaacaaa ctggcgctgg cgctgcgtat gcgtgaccct gactcggaaa aaccactaaa
    18181 cgcgactggc gtacccagcg aagtgcgtcc gttggttgag tcgctaaatc aactgttcgc
    18241 ccgcacacat gcgatgatgg ttcgtgaacg acgctttacc tccgacgcag ctcacgaact
    18301 acgtagcccg ttaacggcgc tgaaagtgca aaccgaagtt gcgcagcttt ctgacgatga
    18361 tccgcaagcg cggaaaaaag cgctgcttca attacattac gggatcgatc gcgctactcg
    18421 tctggttgat caactgctca ctctatcgcg gctggactca ctggataacc ttcaggacgt
    18481 cgcggagatc ccgcttgaag atctcctgca atcgtcggtg atggatattt accacacggc
    18541 gcagcaggcg aaaattgacg tgcgactgac actcaatgcc cacggcatca aacgcaccgg
    18601 acaaccgcta ttgctaagtt tgttggtgcg aaatttgctg gataacgccg tgcgctacag
    18661 tccacagggc agcgtgatag acgtcacgct gaatgctgat aatttcatcg tgagggataa
    18721 cggccccggt gtgacgccag aggctctggc gcgaattggc gaacgcttct atcgcccccc
    18781 cggacaaacc gctaccggca gtggacttgg gctatcgatt gtccagcgaa tcgccaaatt
    18841 gcatggcatg aatgttgagt ttgggaatgc ggaacaaggt ggatttgagg cgaaggtaag
    18901 ctggtaactt ctctgtctat gagagccgtt aaaacgactc tcatagattt tactaatagc
    18961 aaaatataaa ccgtccccaa aaaagccacc aaccacaaac cagacaaata ccgagcgaaa
    19021 atacatatac ccaaaactcg aacatttccc gcataaagag ttttcttaag ataagaataa
    19081 taagtggcgt aagaagaaaa aatgctgcaa tcattgcttg caatatacga tcttcgcctg
    19141 aaacggaagg tatactgcaa atattgtctc ccgactcaag catccagtca tacttattga
    19201 aagcaaaaaa agcatc
//
LOCUS       DAPHDW010000078        19139 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.078, whole genome shotgun sequence.
ACCESSION   DAPHDW010000078 DAPHDW010000000
VERSION     DAPHDW010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 19139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.078"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(215..862)
                     /gene="ychE"
                     /locus_tag="RH057_002800"
     CDS             complement(215..862)
                     /gene="ychE"
                     /locus_tag="RH057_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HDV3129147.1"
                     /translation="MIQTFFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1339..4014
                     /gene="adhE"
                     /locus_tag="RH057_002801"
                     /gene_synonym="adhC"
     CDS             1339..4014
                     /gene="adhE"
                     /locus_tag="RH057_002801"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HDV3129148.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQEADFLANVDKLSEDAFDDQCTGANPRYPLISELKQ
                     ILLDTYYGRDYVEGETAAKKEAAPAKAEKKAKKSA"
     gene            4138..5038
                     /locus_tag="RH057_002802"
                     /pseudo
     CDS             4138..5038
                     /locus_tag="RH057_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904641.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5320..5937)
                     /gene="tdk"
                     /locus_tag="RH057_002803"
     CDS             complement(5320..5937)
                     /gene="tdk"
                     /locus_tag="RH057_002803"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HDV3129149.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            6543..6956
                     /gene="hns"
                     /locus_tag="RH057_002804"
     CDS             6543..6956
                     /gene="hns"
                     /locus_tag="RH057_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HDV3129150.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(7100..8008)
                     /gene="galU"
                     /locus_tag="RH057_002805"
     CDS             complement(7100..8008)
                     /gene="galU"
                     /locus_tag="RH057_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HDV3129151.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(8210..9223)
                     /gene="rssB"
                     /locus_tag="RH057_002806"
     CDS             complement(8210..9223)
                     /gene="rssB"
                     /locus_tag="RH057_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HDV3129152.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(9315..10220)
                     /gene="rssA"
                     /locus_tag="RH057_002807"
     CDS             complement(9315..10220)
                     /gene="rssA"
                     /locus_tag="RH057_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HDV3129153.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            10333..10791
                     /locus_tag="RH057_002808"
     CDS             10333..10791
                     /locus_tag="RH057_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HDV3129154.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMAGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            10841..11683
                     /gene="purU"
                     /locus_tag="RH057_002809"
     CDS             10841..11683
                     /gene="purU"
                     /locus_tag="RH057_002809"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HDV3129155.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            11843..11927
                     /locus_tag="RH057_002810"
     tRNA            11843..11927
                     /locus_tag="RH057_002810"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11877..11879,aa:Tyr,seq:gta)
     gene            11974..12105
                     /locus_tag="RH057_002811"
     ncRNA           11974..12105
                     /ncRNA_class="other"
                     /locus_tag="RH057_002811"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(12289..12966)
                     /gene="narI"
                     /locus_tag="RH057_002812"
     CDS             complement(12289..12966)
                     /gene="narI"
                     /locus_tag="RH057_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV3129156.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRLFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(12966..13676)
                     /gene="narJ"
                     /locus_tag="RH057_002813"
     CDS             complement(12966..13676)
                     /gene="narJ"
                     /locus_tag="RH057_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV3129157.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQAQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(13673..15211)
                     /gene="narH"
                     /locus_tag="RH057_002814"
     CDS             complement(13673..15211)
                     /gene="narH"
                     /locus_tag="RH057_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV3129158.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLEVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(15208..18951)
                     /locus_tag="RH057_002815"
     CDS             complement(15208..18951)
                     /locus_tag="RH057_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HDV3129159.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYCGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLVFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGHLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
BASE COUNT         4832 a         5017 c         4580 g         4710 t
ORIGIN      
        1 atcatttcaa ttattcttca gcaagcccag gattatgatg tggcgaaaat aacatgatgg
       61 tgaggagaag cagttaagga aatccttata actaactgta atttgagaag aatattttta
      121 cctattcctt ttgcttttga taactgcgcg ggaacaaagt aaaaataatg acgaagtaaa
      181 cggagcatgg cagctccgtt tcatttgaaa ggaattaatt aagcaggccg gggaaaatcc
      241 ccttaatacc agtaacgata aattcaatcc ccaatgccat cagcaatagc cccataatac
      301 gcgtaatcac gttgatgccg gtctggcgta aaacccgtac cagccacggt gccatgcgga
      361 acaatcccca gcaacataaa gcgaacaatg caatagccac aaagaaacca aacagatagc
      421 taatgctgtg ataacgcgta ccccagacga tggtagaact gatcgcccct ggccccgcca
      481 tcaacggcaa cgccaatggc accacaccaa tgctttcacg taccgcggtt tctgattttt
      541 cttgcttgtt ctgtttatcc tcgccaagct tgccgctgat catcgacatc gctatcgtca
      601 ccaccaggat gcccccggcg atacggaacg aatcaattga tataccaaaa agttgcagaa
      661 tcgtgtcgcc gagaaaaagc gagatccaca agataatggc cacagacagg ttggctgtaa
      721 ggttagtttt gtttcgcgct gccgctgtct gataactggt catgctgata aagacgggaa
      781 taatccctac cgggttgacc agcgcaaata acccgatgaa aaatttgaag taaacgggaa
      841 aatcaaaaaa ggtctgaatc acggttagct ccgaagcaaa agccggataa tgttagccat
      901 aaataaggtt gaaaagacgc gctgacaata cgccttttga cagcattttt cacctcctaa
      961 ctacttaaaa ttgctatcat tcgttattgt tatctagttg tgcaaaacat gctaatgtag
     1021 ccaccaaatc atactacaat ttattaactg ttagctataa tggcgaaaag cgatgctgaa
     1081 aggtgtcagc tttgcaaaaa tttgatttgg atcacgtaat cagtacccag aagtgagtaa
     1141 tcttgcttac gccacctgga agtgacgcat tagagataat aactctaatg tttaaactct
     1201 tttagtaaat cacagtgagt gtgagcgcga gtaagctttt gattttcata ggttaagcaa
     1261 atcatcaccg cactgactat actctcgtat tcgagcagat gatttactaa aaaagtttaa
     1321 cattatcagg agagcattat ggctgttact aatgtcgctg aacttaacgc actcgtagag
     1381 cgtgtaaaaa aagcccagcg tgaatatgcc agtttcactc aagagcaagt agacaaaatc
     1441 ttccgcgccg ccgctctggc tgctgcagat gctcgaatcc cactcgcgaa aatggccgtt
     1501 gccgaatccg gcatgggtat cgtcgaagat aaagtgatca aaaaccactt tgcttctgaa
     1561 tatatctaca acgcctataa agatgaaaaa acctgtggtg ttctgtctga agacgacact
     1621 tttggtacca tcactatcgc tgaaccaatc ggtattattt gcggtatcgt tccgaccact
     1681 aacccgactt caactgctat cttcaaatcg ctgatcagtc tgaagacccg taacgccatt
     1741 atcttctccc cgcacccgcg tgcaaaagat gccaccaaca aagcggctga tatcgttctg
     1801 caggctgcta tcgctgccgg tgctccgaaa gatctgatcg gctggatcga tcaaccttct
     1861 gttgaactgt ctaacgcact gatgcaccac ccagacatca acctgatcct cgcgactggt
     1921 ggtccgggca tggttaaagc cgcatacagc tccggtaaac cagctatcgg tgtaggcgcg
     1981 ggcaacactc cagttgttat cgatgaaact gctgatatca aacgtgcagt tgcatctgta
     2041 ctgatgtcca aaaccttcga caacggcgta atctgtgctt ctgaacagtc tgttgttgtt
     2101 gttgactctg tttatgacgc tgtacgtgaa cgttttgcaa cccacggcgg ctatctgttg
     2161 cagggtaaag agctgaaagc tgttcaggat gttatcctga aaaacggtgc gctgaacgcg
     2221 gctatcgttg gtcagccagc ctataaaatt gctgaactgg caggcttctc tgtaccagaa
     2281 aacaccaaga ttctgatcgg tgaagtgacc gttgttgatg aaagcgaacc gttcgcacat
     2341 gaaaaactgt ccccgactct ggcaatgtac cgcgctaaag atttcgaaga cgcggtagaa
     2401 aaagcagaga aactggttgc tatgggcggt atcggtcata cctcttgcct gtacactgac
     2461 caggataacc aaccggctcg cgtttcttac ttcggtcaga aaatgaaaac ggctcgtatc
     2521 ctgattaaca ccccagcgtc tcagggtggt atcggtgacc tgtataactt caaactcgca
     2581 ccttccctga ctctgggttg tggttcttgg ggtggtaact ccatctctga aaacgttggt
     2641 ccgaaacacc tgatcaacaa gaaaaccgtt gctaagcgag ctgaaaacat gttgtggcac
     2701 aaacttccga aatctatcta cttccgccgt ggctccctgc caatcgcgct ggatgaagtg
     2761 attactgatg gccacaaacg tgcgctcatc gtgactgacc gcttcctgtt caacaatggt
     2821 tatgctgatc agatcacttc cgtactgaaa gcagcaggcg ttgaaactga agtcttcttc
     2881 gaagtagaag cggacccgac cctgagcatc gttcgtaaag gtgcagaact ggcaaactcc
     2941 ttcaaaccag acgtgattat cgcgctgggt ggtggttccc cgatggacgc cgcgaagatc
     3001 atgtgggtta tgtacgaaca tccggaaact cacttcgaag agctggcgct gcgctttatg
     3061 gatatccgta aacgtatcta caagttcccg aaaatgggcg tgaaagcgaa aatgatcgct
     3121 gtcaccacca cttctggtac aggttctgaa gtcactccgt ttgcggttgt aactgacgac
     3181 gctactggtc agaaatatcc gctggcagac tatgcgctga ctccggatat ggcgattgtc
     3241 gacgccaacc tggttatgga catgccgaag tccctgtgtg ctttcggtgg tctggacgca
     3301 gtaactcacg ccatggaagc ttatgtttct gtactggcat ctgagttctc tgatggtcag
     3361 gctctgcagg cactgaaact gctgaaagaa tatctgccag cgtcctacca cgaagggtct
     3421 aaaaatccgg tagcgcgtga acgtgttcac agtgcagcga ctatcgcggg tatcgcgttt
     3481 gcgaacgcct tcctgggtgt atgtcactca atggcgcaca aactgggttc ccagttccat
     3541 attccgcacg gtctggcaaa cgccctgctg atttgtaacg ttattcgcta caatgcgaac
     3601 gacaacccga ccaagcagac tgcattcagc cagtatgacc gtccgcaggc tcgccgtcgt
     3661 tatgctgaaa ttgccgacca cctgggtctg agcgcaccgg gcgaccgtac tgctgctaag
     3721 atcgagaaac tgctggcatg gctggaaacg ctgaaagctg aactgggtat tccgaaatct
     3781 atccgtgaag ctggcgttca ggaagcagac ttcctggcga acgtggataa actgtctgaa
     3841 gatgcattcg atgaccagtg caccggcgct aacccgcgtt acccgctgat ctccgagctg
     3901 aaacagattc tgctggatac ctactacggt cgtgattatg tagaaggtga aactgcagcg
     3961 aagaaagaag ctgctccggc taaagctgag aaaaaagcga aaaaatccgc ttaatcagta
     4021 gcgctgtctg gcaacataaa cggccccttc tgggcaatgc cgatcagtta aggattagtt
     4081 gaccgatcct taaactgagg cactataacg gcttccacaa cagggagccg ttttcttatg
     4141 ccacttctca atgatctgct cgatttcagt gaccatccgc ttatgcctcc gccctctgca
     4201 caactatttg cagaacacct tcccaccgag tggatacaac actgcctgac gctttctgct
     4261 catgcgaccg ttcgccgccg tcgtttaccg ggggacatgg ttatctggat ggtggtgcaa
     4321 tgagccaatt accgatgttg ttcgccgtct gaacctgagc gcggatggcg aagcggggat
     4381 gaacctgctg gcccgcagcg ctgtcaccca ggcgcgtcag cgcgtggggg ccgccccagt
     4441 ggaatggctc ttccgccaga ccgcacagac aggggcgcgg aacgttacct gaaggatgac
     4501 tggcacggcc tgcaactttt tgccattgat ggcgcacagt tcaggacacc tgactgataa
     4561 acccgagctg cgtgaatatt atgggtctgc caacacatcc actaagcggc agaacgccta
     4621 cccggttatg cgtctggtgg ccttaatgaa cctgggaagt cacattctgc tgaatgccgt
     4681 gaccgcacct taccggcaga gcgaaactgt gctggcccac tccatgctcg ccaccatccc
     4741 ggataactct attacgttgt ttgataaact tttttacagc gaagacctgc tgctgacgct
     4801 gaaccagaag ggttgtaacc gccactggct actgcctgcg tggaagaata tcgcatcaga
     4861 aatgatagag ctgggaaaca cggcgtcacc agggaccatc ccgaaacgac tggagcatct
     4921 tcagggtgct ctggaagtgg tgttcataac aaaacgcccc cgaccatcaa ggccgaggtc
     4981 ggttaagata tcaaaaaccc gttatcctgt gaaacatagt gccgctcctc ttaagtgaac
     5041 ggcattgccc ttctggggcc gtattttttg tttacccaaa gcaacttttc cataaaccga
     5101 cagcattagc cttcatcata tttgcgacga tgtataacgc ctaaacacag ggatattgta
     5161 ctttacaggt cacaagtcaa cgtcggtgct taagagccct gtgaggcgta tagcggcgtt
     5221 aaaaaactgc cgagaagggt atatagcccg gaagaagtgc gtaaaacgaa ctgacaggat
     5281 aaaagtgccc tgctcaccct gtcagtaaag aaattcttat taatcgtggc gatgcctttc
     5341 ctgaatagcc gttaatgagc cgacttgtaa cgcctcttta tagtgtttac ggcatacaga
     5401 aacgtatcgt tcattaccac caattaccac ctgctcacct tcgttataag gtctgcctgc
     5461 ttgatcaaga cgcagcacca tgcttgcttt acggccacaa aaacagatgg tttttaattc
     5521 aaccagtttg tcggaccatg ccagtaagta ttggctgcca ataaataatt cacctcgaaa
     5581 atcggtacgt aaaccataac aaagtacggg tatatcgagt tgatcgacaa cctccgataa
     5641 ttcatatact tgttgtctgg ttaaaaactg gcattcatca accagtacgc aatgaattgc
     5701 ctgctgttca tgttccgcac gaatctcatc aaataatgat gaattttggt taaataattt
     5761 tgcaggcgat gacaaaccta tacgcgaact gactttcccg gcaccaaagc gatcatcaat
     5821 ttctgccgta tatacgacag tgcgcatgcc gcgttcctgg taattgtatg aagattgcaa
     5881 caatgctgta gacttacccg cattcattgc ggaatagtag aaatatagct gtgccatcag
     5941 ccacaggccc tcaatgatat gaaataaagt tggctggagt ttatcataat tcgtcagttt
     6001 ttcagctcaa cagtatgcac agaagtgggt atatgcgttc tcccttacga agccttgcat
     6061 aatccttctg agctatcatt acaactgcct cgcttgttat aagcgggtaa atgactgctg
     6121 gtaactattc acaatcttta acctgttgcg caagtaatag ccctctgttg acctccagga
     6181 gatagtgcaa tactaagtcc atgctcttat tgcgactgtt ctacttttca tcattcgctt
     6241 aatagggaat tctcgtaaac acaactaata cagaagactg aaaggtcgtc agcctacgat
     6301 aatctcccca taaaatgtga catgaatcag gaagttttaa cctcacgtgc tgcgaaatca
     6361 tcggtgtaaa tagggctata tgccgcgtct tttctggcta attttatgaa aagatattta
     6421 ttggcggcac aaaataaaga acaattttga attccttaca ttcctggcta ttgcacaact
     6481 gaatttaagg ctctattatt acctcaacaa accaccccca atataagttt gagattacta
     6541 caatgagcga agcacttaaa attctgaaca acatccgtac tcttcgtgcg caggcaagag
     6601 aatgtacact tgaaacgctg gaagaaatgc tggaaaaatt agaagttgtt gttaacgaac
     6661 gtcgcgaaga agaaagcgcg gctgctgctg aagttgaaga gcgcactcgt aagctgcagc
     6721 aatatcgcga aatgctgatc gctgacggta ttgacccgaa cgaactgctg aatagccttg
     6781 ctgccgttaa atctggcacc aaagctaaac gtgctcagcg tccggcaaaa tatagctacg
     6841 ttgacgaaaa cggcgaaact aaaacctgga ctggccaagg ccgtactcca gctgtaatca
     6901 aaaaagcaat ggatgagcaa ggtaaatccc tcgacgattt cctgatcaag caataatctt
     6961 ttgtagattg cacttgctta aaatcccgcc agcggcggga ttttttattg tccggtttaa
     7021 gacaatttaa taagaataag ctataaaaaa acggcgtcga ttgctcaacg ccgtttcgtg
     7081 gataacaccg atacggatgt tacttcttaa tgcccatctc ttcttcaagc caggctttaa
     7141 attccgtgcc aagggtgtta tgacgaatac cgtattcaac gaaggcctgc atgtaaccta
     7201 atttattacc gcagtcatgg ctcttccctt tcatatgata ggcttccacc gtttcttttt
     7261 cgatcagcat atcaattgcg tcggtgagct gaatttcatc accagctccc ggaggggttt
     7321 ttgccagcaa cggccaaata tccgcgctaa gtacgtaacg acccacaata gcgagattag
     7381 acggcgcaac atccgctttc ggtttttcta ccacaccaac catcggtacg ctttcacccg
     7441 gcgctaattc aacgcctttg caatccacaa cgccatatgc ggtcacatca gcaaccggtt
     7501 caaccatgat ctggctatga cccgtttcat caaagcggcg gatcatctct gccaggttat
     7561 cctgtgacaa atcggattca tattcatcca gaataacatc aggcaaaata acagctaccg
     7621 gttcatcacc cactaccggg tgagcacaca ataccgcgtg tcccaggcct ttcgccagac
     7681 cctgacgaac ttgcataata gtcacgtgcg gtggacaaat agactgcact tcatcaagca
     7741 gttgacgttt tacacgtttt tccagcattg cttccagttc aaaactggta tcaaagtggt
     7801 tttcaataga gtttttagat gagtgtgtaa ccagcacaat ttcagtaatg ccagccgcaa
     7861 tacattcatt cacgacgtat tgaattaatg gcttatcgac aagtggcagc atctctttcg
     7921 ggatggcttt cgtcgccggc aacatcctgg ttcctaatcc cgcaacgggg ataacggctt
     7981 ttttgacttt cgtattaatg gcagccattt aaattctcct ggactgttcg tgttttgaac
     8041 gtgttcatat ttctgtatcg catcccagta tatcagcacc ccgaaggggc taagttctga
     8101 aaaggaggcg agttagcata attaagacaa ttacttatac gaatggcgac gatagtacca
     8161 ccagccgaca ttagcaggta atgcaaattt agcccgcgtt atcgtttgct cattctgcag
     8221 acaacatcaa gcgcagtcga ccaccggttc cccatatttg gcattgccag gcatcgcatc
     8281 gctggctcaa ttgattcaaa taagcgttac ctaaagtgcc taacggaaca ccattactga
     8341 tttgcacctg gtgttcgccg gtatttaacg tcgcattcag acccgcagaa accagaatga
     8401 gatttttcag ttcgcgatga taatagccaa ctaatagcgg aaactgcccc ggcagattgg
     8461 cctgacgaag taaatggttt acctgcttca ataacgcgcc caactctggc aaccgttgat
     8521 tttggtgtgc aagctgttcc tgtaataatc cgttaaacaa tgcgcgtaat aacaaggcag
     8581 caagtacgcc attatgtcca gctcgggtga catcaaggca ataaaatgcc agatcgtttt
     8641 ccgaaagtgc ggcaatatca agcaccaggc cgggtttgtc cgcggcaacc aattgacgat
     8701 aattaacccg gcaatgggaa atcacctgct gaaccggcgg ttgtagttcc tgtaataatt
     8761 tcgccgctgc ggcagggtta tcaaccattg catcccagtc gcgaaaaagc ctttcctctt
     8821 cctcaacgcg cgaattaaac atgctgggat agagacaggc aaaaaccatc tcgcgcaagc
     8881 gattcagatc tttaactggt ttcagcaaaa catcttcaac gcccagacgt aacgctttgg
     8941 caatatctgc catattttca gtggcagata tcaccagaac tggggtctgg tcgcctctgt
     9001 tacgtatatg ctccagcagt ttaagcccgt tcattcgtgg catcgcgata tcacatatca
     9061 tcaggtctgg agtgaaacct cccagcaact caagggcatc caccccatca gccgccagta
     9121 ccgttgtcgc tcccaatgag gaaaaccatg aatccagaag cgagcgaaat acctgctcat
     9181 cttcaacaat gagaatctgt tttccgacca atggctgcgt catgttctct cccctgactg
     9241 gctttactca atagtggcat gctattgcca cctgcgcctg tcagaattaa cttaagtgta
     9301 gataaaaatt ctggtcaaat gttggtgcgt accaacggca aaagttcgtc cattttcctt
     9361 tccactgcca gctgtccggc cgcaatggca gcgtgcgcgc gatggaaatc aagcgtagat
     9421 atttgcgggc aaacaggttg aatcagaata tcgggcggat cacctgccat gcggttcctt
     9481 ttaaggcggt tctccagcac ctggatagaa gtggtcataa tctctgttgc cgttggcgct
     9541 gtcaccgccc gacgtgtcgt tatgctgcct aacctttctt tcagacgcgc atgccacggc
     9601 agagaatcac cattctcgct attttcttca ctgacattaa aggagagcaa atcttgttgc
     9661 atcaaatgag catcgtgctg caggtcaacc gctatcacaa tatcagcccc caatgcacgc
     9721 gtgagggaaa taggaattgg gttaacgact gctccatcaa ccagccagta gccgttatgt
     9781 gcaacaggtg ccatgagtcc tggaatactg catgatgcgc gaatagcaag atggagatcg
     9841 ccttcagtaa accataattc acgtcccgta cttaaattgg tggcaacagc cgcaaagcga
     9901 cgggaacaat tttcgatctc tgtttccggc attatttcgc ggtattgatt gaagacacgc
     9961 tcgccgcgca gtaacccacc gcgctgccag gagagatcca tcaggcgtaa aacatcccaa
    10021 taactgaaag aggtcaccca atcttccagc gcagataatc gatcgcatgc ataggcagcg
    10081 cccaccagcg aaccaattga acatcctgca acgatatcaa tttcaatacc cacttttttt
    10141 agcgcattaa taacgccaat atgcgaccaa cctctcgccg cgccagatcc caacgccagc
    10201 cctattttta tctttctcat tattcccgca agattcccct ggaatgcaat cgtagccaca
    10261 tcgctactgc tcggttaaca tagtgctact caggcggtta atacgccgtt attgtttcca
    10321 gggagattat ttgtgtctca gctttgtccc tgtggtagtg ctgtcgagta tagcctatgt
    10381 tgccaccctt atgtgtctgg tgaaaaggtt gcacctgatc cagaacatct catgcgttcg
    10441 cgttactgcg cttttgtgat gcaagacgca gattatttaa taaagacctg gcatccctct
    10501 tgtggagcag cggcgttacg tgccgaattg atggccggat ttgcgcatac tgaatggttg
    10561 ggattgaccg tatttgaaca ttgttggcag gacgccgaca atatcggatt tgtcagtttt
    10621 gttgctcgat ttaccgaagg tggtaaaaca ggtgcaataa ttgaacgttc gcgatttttg
    10681 aaagaaaacg gtcaatggta ctatattgac ggtacacgtc cgcagtttgg tcgcaacgat
    10741 ccctgccctt gtggttcagg taaaaaattt aaaaagtgct gcggccaata atggttgacg
    10801 gtacggttta gcaaacactc tcaacaaggt ttttccagca atgcattcac tccaacgtaa
    10861 agttctgcgt actatttgtc cggaccaaaa aggtctgatc gcacgtatta ccaatatttg
    10921 ctacaagcac gagttaaata tcgtacagaa caatgaattt gttgatcacc gtaccgggcg
    10981 cttttttatg cgcacggaac tggaagggat ttttaatgat tccaccctgc tggcggatct
    11041 cgatagcgca ttgccagaag gctccgtgcg tgagctgaat cctgccggtc gtcgccggat
    11101 agtgattctg gtcactaaag aagcgcattg ccttggcgat ttgttgatga aagccaatta
    11161 cggcggcctg gatgtcgaaa tcgcggcagt tattggtaac cacgatactt tacgttctct
    11221 ggttgagcgt tttgatattc cgtttgagct ggtaagccat gaagggttaa gccgcaacga
    11281 gcacgatcaa aagatggcgg acgccattga tgcttatcaa cctgactatg tggtgctggc
    11341 gaagtatatg cgggtattaa cgccggaatt tgtggcacgc ttcccgaata aaatcatcaa
    11401 tattcaccat tcattcctgc ctgcgtttat cggcgcacgt ccttatcacc aggcctatga
    11461 acgtggtgtg aagattattg gcgcaaccgc tcactatgtg aatgacaatc tggacgaagg
    11521 cccaatcatc atgcaggacg ttattcatgt cgatcatacc tacacagctg aagatatgat
    11581 gcgcgcaggt cgtgacgtcg agaaaaacgt cttaagtcgt gcgctataca aagtactggc
    11641 acagcgcgtc tttgtttacg gtaatcggac gattattctt taatcgccag caaaaataac
    11701 tggttacctt taatccgtta cggatgaaaa ttacgcaacc agttcatttt tctcaacgta
    11761 acactttaca gcggcgcgtc atttgatatg atgcgccccg cttcccgata agggagcagg
    11821 ccagtaaaaa gcattacccc gtggtggggt tcccgagcgg ccaaagggag cagactgtaa
    11881 atctgccgtc atcgacttcg aaggttcgaa tccttccccc accaccatca ctttcaaaag
    11941 tccgtgaact ctcaaacgaa tccgcgatta aatattctgc ccatgcgggg aaggatgaga
    12001 agcctcgacc aaggttcgac tcgagcgcca gcgagagagc gttgccgcag gcaacgaccc
    12061 gaagggcgaa gcgcgcagcg ttgagtaatc cttcccccac caccatcact tccttaaata
    12121 atcaaaacct gttactccga gctaattttg cccgatggca ccacgtttac cgggcctaca
    12181 atatcctccc ctgtaggtcg gataagcgaa gcgcatccga caactcaccc cgcaccgtgc
    12241 ccataaaaaa accccgccga agcagggtct atgtgaacta aaattcgctt agtgacgagc
    12301 gcgcaccagc tggtacttac gtgtcagata ctccaccggt acgctccaga tgtgtaccag
    12361 acgcgagaac gggaacagca ggaataacgt catccccagc accaggtgca gacggaagat
    12421 aaacgccaca ccatcgaggt gttgagaagc gccaccgtgg aaggtcacca ccgactgcgc
    12481 ccagccaacc agtttcatca tctcgctacc gtccatatgc tgagcggaga acggaatggt
    12541 caacaggccc agcgcgcact ggataacgag cagcgacagg atcaggatat ccgctccggt
    12601 agtggttgca cgcacgcgtg ggctgaacag acgacgcttc agcaacagca cgccgccaat
    12661 cagacacagc acgccgctgg caccaccagc aaacatcgcc attttctgtt tcacttcaat
    12721 cggcagccag gcttcataca tccagtgcgg cgtcagcata ccgaagaagt gaccaacaaa
    12781 aatccccaga ataccgatat ggaacaggtt cgacgccagg ttcatccctt tgcgatccag
    12841 catctggctg gacgccgcgc gccaggtgta ctgcccgtag tcataacgca gccagctacc
    12901 aatcaggaag accgccccgg ctatgtacgg gtagatatca aagaagaaca tattcaggaa
    12961 ttgcattagt gctgtcctcc ggtggtgata ttcagatatt gcggcgcgac ggcaccggca
    13021 aagcgacgct gatgagcagt gattgctgaa tcaccgcagc ctttgtcagc aaagaattta
    13081 acctgctctt cttcccagac agcatccagc gcctgcggcg tatcatcgcg cgcttcgtcg
    13141 gcaatttttt ccgccacttt gtcgctgtcg atagcggtat tggccaattt cagcagcaga
    13201 tcaaacatca cggcataacg gctttcacgc tgttgcagac gcgcgctcag caatgccaga
    13261 atcggcgcga tatctttcaa accttccacg gcttcgcttt gcggcagctg cgacaggtac
    13321 tccagataca gcggcagatg gtccggtaat tcgcggctgt ttaactgcaa gccgtgctgc
    13381 tcgtactgcg ccagcaggtc caccatcgcc tggccgcggt cacgggattc accgtgaaca
    13441 tgttcaaata gcaacagtga cgtggcgcgg ccacggtcga acagttcgct gtactgggcc
    13501 tgggcatcga gcggatccat cgccgttaaa tcgcgcagga aaatgcccag cgcatgggca
    13561 tcttctttcg acagattttt cgacgcagca atcgcctcaa acatctcttg ttgatgctgc
    13621 cataaggcag catccggata ttcaaggaga cgcgatacaa tcacgagttc gatcatggat
    13681 gcggctccgt tttgctggtc acatcgatgg catcgatacg acggctgttg aacagattga
    13741 atttggtatc tgaaccgtgg cagccatcac caaaggtaaa gccgcagcca tttttctccg
    13801 ggaaggcttc ccgtgccagt tcacgatgac tactcggcac cacaaagcga tcttcgtagt
    13861 tagcaatcgc cagataacgg tacatctcct gtgcctgggc ttcggtcaga ccgacctctt
    13921 ccagcgcacg ggtatcaact ttaccgtcaa cggtttcagc acgtttgtaa tgacgcatcg
    13981 ccagcatacg tttcagtgcg cgcagtaccg gtttggtatc accggcggtc agcagattcg
    14041 ccagatactg taccggaata cgcaagcttt cgacgtctgg cagaatgccg ttgctaccca
    14101 gctcacccgc gtctgccgca gactgaatcg gagacagagg cggcacgtac cagaccatcg
    14161 gcagtgtgcg atattctgga tgcagcggca gcgccagttt ccattccatt gccattttat
    14221 aaaccggcga ctgctgtgcg gcttcaataa cgctcagcgg aataccgtct ttaatcgcct
    14281 gctcgatgac tttcggatcg ttcggatcga ggaacacctc cagctgacgc tggtaaagat
    14341 ctttctcgtt ctcggtgctg gctgcacgtt caatcgcgtc ggcatcgtac agcagcacgc
    14401 caagataacg gatacgaccg acacaggttt ctgagcacac ggtcggctga cccgcttcaa
    14461 tacgcggata gcagaagatg cacttctcag acttaccgct cttccagttg aagtagattt
    14521 ttttgtacgg gcatccggtg atgcacatac gccagccacg gcatttatcc tggtcgatca
    14581 ggacgatgcc atcttcttca cgcttgtaaa tcgcaccgct cgggcaggtc gccacacatg
    14641 ccgggttcag gcagtgttcg cacaggcgcg gcaaatacat cataaaggtg ttttcgaact
    14701 ggctatacat cgccttctgg atgttgtcga agttcttgtc tttcgccagt ttgtcaaact
    14761 caccacccag gtcatcttcc cagttcggcc ctttttcgat tttcgccatc cgttccccgg
    14821 taatcagcga acgcggacgg gcaatcggct gcgatttgct gccttccggc gcggtatgca
    14881 ggttctgata gtcaaaatcg aacggctcgt aataatcgtc gatccccggc agatgcgggt
    14941 tagcgaagat tttacccagc aacatggcac ggttacccat gcgcggctgc agtttgccgt
    15001 tgattttacg gatccagccg cctttgtatt tttcctggtt ttcccagtca gtcgggaagc
    15061 cctgacccgg cttggtttcc acgttgttga accacgcgta ttccacgcct tcacggctgg
    15121 tccagacgtt tttacaggta actgaacagg tgtggcagcc gatgcactta tcgagattca
    15181 gcaccatgcc gacttgtgaa cgaattttca ttttacgctc tcctgtacct ggtcattgcc
    15241 ttcgccatct aaccagtcaa tgttcttcat cttacgcact acaacaaact catcgcggtt
    15301 agaacctacg gtgccgtagt agttaaagcc gtatgccaga tgggcatagc cgccgatcat
    15361 atgcgtcggt ttcggcgtga tacgggtgac cgagttatgg ataccaccac gctgttgggt
    15421 aatttccgaa ccaggcaggt taacgatacg ttcctgcgcg tggtacatca tggtcatccc
    15481 tgccggaaca cgctggctga caacagcacg ggcagtcaga gcaccgttgc tgttgaatac
    15541 ttcaatccag tcgttatcgg cgatacccag atctttggca tcggcttcac tcaaccagac
    15601 caccggacca ccgcgaccta aagtcagcat cagcaggttg tcgctgtagg tggagtggat
    15661 accccacttc tggtgcggcg tcaggaagtt gagcgctttt tccgggttgc cgttggattt
    15721 ctggcctatc acttctttca ccgaacgggt gtcgatcggc ggacgataaa ccagcaggct
    15781 ttcaccgaaa tcacgcatcc actggtgatc ctgatacagt tgctgacgac cagagagcgt
    15841 acgccatggg atcagctcgt gaacgttggt gtaaccggcg ttgtaagaaa cgtgttcatc
    15901 ttccagacca gaccaggtcg ggctggagat aattttgcgc ggctgtgcct gaatatcgcg
    15961 gaagcggatc ttctcgtctt ctttattcag cgccagatgc gtatggtcac gaccggtaaa
    16021 ttcgctcagg gcagcccagg ctttcacggc tacctgaccg ttggtttccg gtgccagtgt
    16081 caggatcatc tctgccgcat caattgcggt gttcagcatc ggctggcctt tcgccggacc
    16141 ttccgctttg gtgtagttga gcttacgcag cagatccatc tcgctctggg tgttccaggc
    16201 aatcccttta ccgccattac cgattttctc catcagcggg ccgatagagg taaagcgttc
    16261 gtaagtcgcc ggataatcgc gctctacgac cataatgtgt ggcgcggttt tacctgggat
    16321 caggtcgcac tcgccttttt tccagtcttt cacatccagc ggctgcgcca gttcagcggc
    16381 agagtcatgc tggataggca gcgtgacgat gtcggtttct ttacccagat ggccgacgca
    16441 cacttcggag aatttcttcg cgatggcttt gtagatttcc cagtcgcttt tcgcttccca
    16501 ggccggatcg accgccgcag acagcgggtg aataaacgga tgcatatccg aagtattcat
    16561 gtcgtctttt tcgtaccagg tcgccgtcgg caaaatgatg tcggaataga gacaggtgct
    16621 cgacagacgg aagtccagcg tcaccaccag atccagcttg ccttccagac cattgtcctg
    16681 ccagtccact tcttccggct tcacgccgcc ctgttgcccc agatctttac cctggatacc
    16741 gtgctccgtc cccagcaggt acttgagcat aaactcatga cctttaccgg aagaaccgag
    16801 caggttagaa cgccagatga acaggttgcg cgggtggttt ttaccgtttt ctggttgttc
    16861 tgccgcaaaa cggatggaac cctctttcag ggatttcacc gtatagtcca ccggattcat
    16921 cccggctttt tctgcttcgc cagcgatagt cagcgggtta gtgcctaact gcggcgcaga
    16981 cggcagccag cccatgcgtt ctgcacggac gttaaagtcg atcaggtgtc cggtataacg
    17041 ggatttatcc gccatcggcg acagcagttc ttccgcagtc acggtttcat aacgccactg
    17101 gctggagtga ttatagaaat aagaagtgct gttcatatga cgcgccggac gctgccagtc
    17161 aagggcaaac accagcggct gccagccggt ttgcggacgc agtttttcct gacctacata
    17221 gtgcgcccag ccgcccccgc tctgaccgac acagccgcag aaaatcagca tgttgatcag
    17281 accgcgatag ttcatatcga ggtgatacca gtggttcagc cccgcaccga cgataatcat
    17341 cgaacgaccg tgcgttttat cagcgttatc ggcaaattca cgggcgatgc gaataatctg
    17401 gctgcgagaa acgccggtga tctgctcagc ccaggccggg gtgtaagctt tcacatcgtc
    17461 atagctggtt gcacagttaa cgtcgttcag gccacgttcc agaccgtagt ttgccagcgt
    17521 cagatcataa acggtggtca ccagggcggt gctgccatca gccagttgca ggcgtttcac
    17581 cggcagtttg tgcagcagca cgttttccag ttccactttg ttgaagtgtt ccgtgccgtc
    17641 gccaccacag tacgggaagc ctacctcagc gatctcatcc tggctaccca gcaggctcag
    17701 ttgcagctcg gtttcttcgc cagttttgcc gtcgcgctgc tcaagattcc atttgccctt
    17761 ctcgccccag cggaagccaa tagaaccgtt cggcgcaacc atttcgccat tggtattaaa
    17821 ggcgacagtt ttccattccg gattgttttc ctggcccagc gcatcaacca gatcagcagc
    17881 gcgcagcata cgacctgcag cgtagtaacc gtcgcgttct tccagcatca ccagcatcgg
    17941 catgtcggtg tagcgacgca catagtcggt gaaatactgg cttgggttgt cgaggtggaa
    18001 ttcacgcagc attacgtggc ccatcgccag cgccattgcc gcatcggtgc cctgtttcgg
    18061 tgccagccac agatcgcaca gtttggcgat ttcagcgtag tctggtgtga cggcaacagt
    18121 tttggtccct ttgtaacgca cttcagtaaa gaagtgagca tccggggtac gcgtctgcgg
    18181 tacgtttgac ccccaggcga tgatgtagct ggagttgtac cagtcagcag attccggtac
    18241 gtcagtttgc tcgccccagg tttgcggaga cgcaggaggc aagtcgcagt accagtcgta
    18301 gaagcttaag caagtaccgc caatcagcga gagatagcgt gcacccgatg cgtaagaaac
    18361 catcgacatt gccggaattg gcgagaaacc agcaacacgg tccgggccgt agtttttgat
    18421 ggtgtaaacg ttagatgcgg cgatcagttc gttcacctcc tgccaggaag aacgaacaaa
    18481 tccaccgcgt ccacgcgcct gcttaaagct tttcgcttta tcggcgtctt caatgataga
    18541 agcccatgcc tcaaccggat cgctatgcag cgccttcgct tcacgccaca ttttcatcag
    18601 gcgtttgcgc atcatcgggt atttcaggcg gttggcgctg taaagatacc aggagtagct
    18661 ggcaccgcgc gggcagccgc gaggttcatg gtttggcaga tccggacggg tacgcggata
    18721 gtcagtctgc tgggtttccc aggtgaccag accgtttttg acgtagattt tccagctgca
    18781 ggagccggtg cagtttaccc cgtgggtaga gcggacgatt ttgtcatgct gccaacgctg
    18841 gcgatatcca tcctcccagt cacggttggt attgagaagc tggccatgcc catcggcaaa
    18901 ggtttcaccc ttctgtttga agtagcgaaa ccggtccagg aatttactca tcggttttct
    18961 cctgtgggag cctgtcggcc ctctgataaa tcgacattgc taatgtttaa gggcaaggta
    19021 acgctctgaa acagcgtggg aattgataac gatcaagagt gatggggatg aaaaataaag
    19081 taaattccta agatacccct ttaggataat taacagccac cacataacag actgtaaat
//
LOCUS       DAPHDW010000079        18920 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.079, whole genome shotgun sequence.
ACCESSION   DAPHDW010000079 DAPHDW010000000
VERSION     DAPHDW010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18920)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18920)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18920
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.079"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            208..657
                     /gene="alaE"
                     /locus_tag="RH057_002816"
     CDS             208..657
                     /gene="alaE"
                     /locus_tag="RH057_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HDV3129160.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(694..1038)
                     /locus_tag="RH057_002817"
     CDS             complement(694..1038)
                     /locus_tag="RH057_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HDV3129161.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            1190..1519
                     /locus_tag="RH057_002818"
     CDS             1190..1519
                     /locus_tag="RH057_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV3129162.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVGCADSFVRERPWCSVGTAAAVGIFIGA
                     LLSMRKS"
     gene            1767..2012
                     /gene="nrdH"
                     /locus_tag="RH057_002819"
     CDS             1767..2012
                     /gene="nrdH"
                     /locus_tag="RH057_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HDV3129163.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            2009..2419
                     /gene="nrdI"
                     /locus_tag="RH057_002820"
     CDS             2009..2419
                     /gene="nrdI"
                     /locus_tag="RH057_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HDV3129164.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIAQKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            2392..4536
                     /gene="nrdE"
                     /locus_tag="RH057_002821"
     CDS             2392..4536
                     /gene="nrdE"
                     /locus_tag="RH057_002821"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HDV3129165.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     MATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFHTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPNFARTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASSEYFSQYLQGNWQPKTTKVGELFARSGITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            4546..5505
                     /gene="nrdF"
                     /locus_tag="RH057_002822"
     CDS             4546..5505
                     /gene="nrdF"
                     /locus_tag="RH057_002822"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HDV3129166.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNANKALMNLDYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            5860..7062
                     /gene="proV"
                     /locus_tag="RH057_002823"
     CDS             5860..7062
                     /gene="proV"
                     /locus_tag="RH057_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HDV3129167.1"
                     /translation="MAIKLEIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            7055..8119
                     /gene="proW"
                     /locus_tag="RH057_002824"
     CDS             7055..8119
                     /gene="proW"
                     /locus_tag="RH057_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HDV3129168.1"
                     /translation="MADQNNPWDTTPAADSAVQSADAWGTPATAPTDGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALIAWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            8176..9168
                     /gene="proX"
                     /locus_tag="RH057_002825"
     CDS             8176..9168
                     /gene="proX"
                     /locus_tag="RH057_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HDV3129169.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            9459..10643
                     /locus_tag="RH057_002826"
     CDS             9459..10643
                     /locus_tag="RH057_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3129170.1"
                     /translation="MTKPNHELSPALIVLMSIATGLAVASNYYAQPLLDTIARNFSLS
                     ASSAGFIVTAAQLGYAAGLLFLVPLGDMFERRRLIVSMTLLAAGGMLITASSQSLAMM
                     ILGTALTGLFSVVAQILVPLAATLASPDKRGKVVGTIMSGLLLGILLARTVAGLLANL
                     GGWRTVFWVASVLMALMALALWRGLPQMKSETHLNYPQLLGSVFSMFISDKILRTRAL
                     LGCLTFANFSILWTSMAFLLAAPPFNYSDGVIGLFGLAGAAGALGARPAGGFADKGKS
                     HHTTTFGLLLLLLSWLAIWFGHTSVLALIIGILVLDLTVQGVHITNQTVIYRIHPDAR
                     NRLTAGYMTSYFIGGAAGSLISASAWQHGGWAGVCLAGATIALVNLLVWWRGFHRQEA
                     AN"
     gene            10767..11504
                     /gene="ygaZ"
                     /locus_tag="RH057_002827"
     CDS             10767..11504
                     /gene="ygaZ"
                     /locus_tag="RH057_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HDV3129171.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALTVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            11494..11829
                     /gene="ygaH"
                     /locus_tag="RH057_002828"
     CDS             11494..11829
                     /gene="ygaH"
                     /locus_tag="RH057_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HDV3129172.1"
                     /translation="MSYEVLLLGLLVGVANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            11920..12450
                     /gene="emrR"
                     /locus_tag="RH057_002829"
     CDS             11920..12450
                     /gene="emrR"
                     /locus_tag="RH057_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HDV3129173.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRESDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            12577..13749
                     /gene="emrA"
                     /locus_tag="RH057_002830"
     CDS             12577..13749
                     /gene="emrA"
                     /locus_tag="RH057_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
                     /protein_id="HDV3129174.1"
                     /translation="MSANAETQTPQQPVKKSGKRKRLLLLLTLLFIIIAVAIGIYWFL
                     VLRHFEETDDAYVAGNQIQIMSQVSGSVTKVWADNTDFVKEGDVLVTLDPTDARQAFE
                     KAKTALASSVRQTHQLMINSKQLQANIEVQKIALAKAQSDYNRRVPLGNANLIGREEL
                     QHARDAVTSAQAQLDVAIQQYNANQAMILGTKLEDQPAVQQAATEVRNAWLALERTRI
                     VSPMTGYVSRRAVQPGAQISPTTPLMAVVPATNMWVDANFKETQIANMRIGQPVTITT
                     DIYGDDVKYTGKVVGLDMGTGSAFSLLPAQNATGNWIKVVQRLPVRIELDQKQLEQYP
                     LRIGLSTLVSVNTTNRDGQVLANKVRSTPVAVSTAREISLAPVNKLIDDIVKANAG"
     gene            13766..15304
                     /gene="emrB"
                     /locus_tag="RH057_002831"
     CDS             13766..15304
                     /gene="emrB"
                     /locus_tag="RH057_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HDV3129175.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLDSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDWFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFGASAWPQFIQGFAVACFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(15368..15883)
                     /gene="luxS"
                     /locus_tag="RH057_002832"
     CDS             complement(15368..15883)
                     /gene="luxS"
                     /locus_tag="RH057_002832"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HDV3129176.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGKGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILERDVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(16033..17589)
                     /gene="gshA"
                     /locus_tag="RH057_002833"
     CDS             complement(16033..17589)
                     /gene="gshA"
                     /locus_tag="RH057_002833"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HDV3129177.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLPQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMEAADTE
                     PFAVWLEKHA"
     gene            complement(17662..18090)
                     /locus_tag="RH057_002834"
     CDS             complement(17662..18090)
                     /locus_tag="RH057_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3129178.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISYPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(18087..18653)
                     /gene="yqaB"
                     /locus_tag="RH057_002835"
     CDS             complement(18087..18653)
                     /gene="yqaB"
                     /locus_tag="RH057_002835"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HDV3129179.1"
                     /translation="MYERYAGLIFDMDGTILDTEPTHRKAWREVLGHYGLQYDVQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
BASE COUNT         4495 a         4894 c         4781 g         4750 t
ORIGIN      
        1 atcatatcgg catgtaaaaa tccacaatgt acaaaaaacg agccgttacg gaatatttta
       61 tctacaaaaa ctgactaaat aaaaattttt cactaattga atagtcatag ccagcgatat
      121 acgctatgcg aaaatgcaga tgacaatgag atccactgct ttcatctcca ttaacatccc
      181 attacgcttt tattaaggag cattagcatg ttctcaccgc agtcacgctt gcgtcatgca
      241 gttgcagata cgttcgcgat ggttgtttac tgttctgtcg tgaacatgtg tattgaagtt
      301 ttcctctccg gaatgagctt cgaacagtct ttttattcca gattggtagc gattccggtg
      361 aacatcttaa ttgcatggcc atacggtatg taccgcgatc tgtttatgcg cgcggcacgc
      421 aaagttagcc cgtcgggctg gataaaaaat ctggctgata tcctggctta tgtgacgttc
      481 cagtcaccgg tgtatgtggc gatcttgtta gtggtgggcg cagactggca tcagattatg
      541 gcggcggtca gttcaaacat cgttgtttcg atgttgatgg gggcggttta tggctacttc
      601 ctcgattatt gccgccgact gtttaaagtc agccgttacc agcaggtaaa agcctgactc
      661 ttcttttcgc gactggcatc gccagtcgcg tctttaactg gcttcgccaa ataaaccatt
      721 caaataacgt tcaagcgcaa cacgcgaact gaagccatgc gggataccgt aatgctcgtg
      781 tcgctcgcct gctaaataga gcggaaattg ctggtaatga tcgcaagttt taatatcgga
      841 agcgggttca gccagtgttg aactgcgttc ttttaatgtt ggatgaatta ccagagcggt
      901 acgtcccatt cgcgcttcgc gattaacata gacataattt tccccacggc ggtaaccata
      961 cgctttctgg gttaccacat cgacagtaaa accgactttt tcaagtacgc gcgccacctc
     1021 gtctggtcgt aaatacatat ttgatcctcg ttattatcaa ccgcgggcct accttacctg
     1081 attgcgcatt cacaacgctt tcagaaaagt ccataaagcc gcgatgaacc tcagttaaga
     1141 aaattatggt ctacactgaa aattacatcg aattctaatg ggggatcata tgtttaaccg
     1201 accgaaccgc aacgacgttg atgatggcgt gcaggatatc cagaatgatg tcaatcaatt
     1261 agctgacagc ctcgaatctg tattaaagtc ctggggcagc gacgccaaag gggaagctga
     1321 agccgcacgc agtaaagctc aggcattgct gaaagaaacc agggcgcgaa tgcatggtcg
     1381 tactcgcgtc cagcaagccg cacgcgatgc cgttggctgc gcggattctt ttgttcgtga
     1441 aagaccctgg tgtagcgtgg gtacagcagc tgcggtaggt atttttatcg gcgcactgtt
     1501 aagcatgcgc aaatcgtagt gcaaaaatga taataaatac gcgtctttga ccccgaagcc
     1561 tgtcttcggg gtttcttttt gcctggtgaa tcacaaaaat ccccctaccc cgtcacgctc
     1621 atatccaggg taatttcgac cactatttgc tatatattgt gtggttgaat cttttttcaa
     1681 ctacatctag tatctctgta tcaacagaga gacaacccga cgcgtatcat cgcgccgtat
     1741 cttcatttta aacggaaata cgaatcatgc gcattactat ttacactcgt aacgattgcg
     1801 ttcagtgcca cgccaccaaa cgggcgatgg aaaaccgggg ctttgatttt gaaatgatta
     1861 atgtcgatcg cgttcctgaa gcggcggaag cgctgcgtgc tcagggcttt cgtcagttgc
     1921 cggtagtgat tgctggcgat cttagctggt ctggtttccg cccggacatg atcaatcgtc
     1981 tgcatccagc gccacacgcg gccagtgcat gagccagctc gtctactttt ccagtagctc
     2041 cgaaaatacg cagcgtttta tcgaacgttt aggactgccc gcggtgcgca tcccgctcaa
     2101 tgagcgggaa cggattcagg tagacgagcc ttacatcctg atcgtgccct cttacggcgg
     2161 cggcggtacg gctggcgcgg tgccacgaca ggtaattcgc tttttaaacg acgagcacaa
     2221 ccgggcgttg cttcgcggcg ttattgcttc gggtaatcgc aactttggtg aggcgtatgg
     2281 ccgtgccgga gatgtgattg ctcagaaatg cggcgtgccg tggctgtacc gttttgaact
     2341 catgggtacg caaagcgata tcgaaaacgt tcgtaaagga gtaaccgaat tttggcaacg
     2401 acaaccgcag aatgcctgac gcaggaaacg atggattacc acgcgctgaa tgcgatgctt
     2461 aacctctacg atagcgcagg tcgcattcag ttcgataaag accgccaggc cgttgacgcc
     2521 tttatggcga cgcatgtgcg tccgaacagt gtgaccttca gtagccagca gcagcgcctg
     2581 aactggctgg tcaacgaagg ttactatgat gaaagcgttc ttaatcgcta ctctcgcgac
     2641 tttgtcatta cgctgtttgc ccacgcacac accagcggtt ttcgtttcca tacattcctc
     2701 ggggcatgga agttttacac cagctatacg ttgaagacat tcgacggtaa gcgttatctg
     2761 gaagattttg ccgatcgagt cacgatggtg gcgctaacgc tggcacaagg cgatgaaaca
     2821 ctggcgttgc aactgaccga tgagatgctg tcaggacgct ttcagcctgc aacaccgaca
     2881 ttcctcaact gtggtaagca gcagcgcggc gaactggttt cctgtttttt gctgcgtatt
     2941 gaagacaata tggagtcgat tggccgggcg gtaaattccg cactgcaact gtcgaaacgc
     3001 ggcggcggcg tagcattttt gctgtcgaat ctgcgggaag cgggcgcgcc aattaaacgt
     3061 attgaaaatc aatcttctgg cgtaattccg gtgatgaaaa tgctggaaga cgcattttcc
     3121 tatgccaacc aactcggcgc tcgtcagggg gctggtgcag tctatttaca tgctcatcat
     3181 cccgatattc tgcgttttct cgacacgaaa cgggaaaatg ccgacgaaaa aatccgcatt
     3241 aaaacactgt cgcttggcgt ggtgatcccg gatatcactt tccatctggc caaagagaat
     3301 gcgcagatgg cgctgttttc gccctatgac gttgagcgag tttatggcaa gccgtttgcc
     3361 gatatcgcca ttagcgaaca ctatgacgaa ctggttgccg atgaacgcat ccgcaaaaaa
     3421 tacctcaacg cccgtgattt cttccagcga ctggcagaaa tccagtttga gtccggctat
     3481 ccctacatca tgtatgaaga cacggtaaac cgtgctaacc ctatcgccgg gcgcataaat
     3541 atgagtaatc tatgctcaga aattttgcag gttaacagcg cctcagagta tgacgagaat
     3601 ctcgactatg cccgtacagg ccatgatatt tcctgcaatt taggttcgtt gaatattgcc
     3661 cacaccatgg attcccccaa ctttgcccgc acggtagaga ctgccgtacg cggtttaacg
     3721 gcggtgtcag atatgagtca tatccgcagc gtgccgtcca tcgaagccgg aaatgccgcc
     3781 tcacacgcca tcgggctggg gcagatgaat ttacacggtt atctggcgcg agaaggcatc
     3841 gcttatggtt cgccggaagc actggatttc accaatctct atttctatac catcacctgg
     3901 cacgcactgc gtacctcgat gttgctggca cgcgaacgcg gtgaaacctt cgccgggttc
     3961 aaacagtcac gctatgccag tagtgaatat ttcagccaat atctgcaagg gaactggcag
     4021 ccgaaaacga cgaaagttgg cgaactgttt gcccgtagcg gtattacgtt acctacccgt
     4081 gagatgtggg cgcaactgcg cgacgacgtg atgcgctacg gcatttataa ccagaatctt
     4141 caggcggtgc cgccaaccgg ttctatctct tatatcaacc atgctacgtc gagtattcat
     4201 ccgattgtgg cgaaagtaga gatacgcaaa gagggcaaaa caggacgcgt ttactaccct
     4261 gccccgttta tgactaacga gaatctggcg ctgtatcagg atgcttacga aatcggcgca
     4321 gaaaagatca tcgacaccta cgcggaagcg actcgccatg tcgatcaggg tttgtcgctg
     4381 acgctgtttt tccccgatac cgccaccact cgcgatatca acaaagcgca gatttacgcc
     4441 tggcgcaaag gtatcaaaac gctctattac attcgcctgc gtcagatggc gctggaaggc
     4501 actgaaattg aaggctgcgt ctcctgtgca ctttaaggaa tatctatgaa actctcacgt
     4561 atcagcgcca tcaactggaa caagatatct gacgataaag atctggaggt gtggaatcgc
     4621 ctgaccagca atttctggct accagaaaag gtgccgctgt cgaacgatat tcctgcctgg
     4681 cagacattaa ctgtcgtaga acaacaactg acgatgcgcg tttttactgg cctgacgctg
     4741 ctcgacacgc tgcaaaatgt tatcggcgcg ccttcgctga tgcccgatgc actcacgcct
     4801 catgaagaag cggtattatc gaatatcagc tttatggaag cggttcatgc ccgctcttac
     4861 agttcgattt tctcgacgct gtgtcagacc aaagatgtcg atgccgccta cgcatggagt
     4921 gaagaaaacg caccgttgca gcgaaaagct cagattattc agcaacatta tcgcggtgat
     4981 gatccgctga aaaagaaaat cgccagtgtg tttcttgaat cttttttgtt ctattccggt
     5041 ttctggctgc cgatgtattt ttccagccgc ggaaagctga ccaatacagc agatctgatc
     5101 cgtctgatta tccgcgatga agcagtccac ggttactaca taggctataa atatcagaaa
     5161 aacatggaaa agatatctct gggacaacgt gaagagttga agagtttcgc cttcgatttg
     5221 ttgctggaac tctacgacaa cgagttgcaa tacaccgatg agctgtacgc cgaaaccccg
     5281 tgggctgacg atgtgaaagc gtttctctgt tacaacgcca ataaggcttt gatgaatctg
     5341 gactacgaac cgttatttcc cgcagaaatg gcggaagtga atccggcaat cctcgccgcg
     5401 ctttcgccga atgccgatga aaatcacgat ttcttttccg gttcaggctc ctcttatgtg
     5461 atggggaaag cggttgaaac agaagatgaa gactggaatt tctgagggtg ttattttcaa
     5521 aaatatcact acccgcagca gggaaataat tcccgccaaa tagcttttta tcacgcaaat
     5581 attctgtagt gatctacact gatactctgt tgcattattc gcctgaaact acaatattca
     5641 ggcgtttttt cgctatctat gacaaaaaat atcaactttt ctcgatttgc tctcagccct
     5701 tatatcacgg gaaattccgg cgatttgctc gcatcaatat tcatgccaca tttgccctca
     5761 ggggttgcct cagattctca gtatgttagg gtagaaaaaa gtgactattt ccattgggta
     5821 atatatcgac ataaacaaat aaaggaatct ttctattgca tggcaattaa attagaaatt
     5881 aaaaatcttt ataaaatatt tggcgagcat ccacagcgag cgttcaaata tatcgaacaa
     5941 ggactttcga aagaacaaat tctggaaaaa actgggctat cgcttggcgt aaaagacgcc
     6001 agtctggcca ttgaagaagg cgagatattt gtcatcatgg gattatccgg ctcgggtaaa
     6061 tccacaatgg tacgccttct caatcgcctg attgaaccca cccgcgggca agtgctgatt
     6121 gatggtgtgg atattgccaa aatatccgat gccgaactcc gtgaggtgcg cagaaaaaag
     6181 attgcgatgg tcttccagtc ctttgcctta atgccgcata tgaccgtgct ggacaatact
     6241 gcgttcggta tggaattggc cggaattaat gccgaagaac gccgggaaaa agcccttgat
     6301 gcactgcgtc aggtcgggct ggaaaattat gcccacagct acccggatga actctctggc
     6361 gggatgcgtc aacgtgtggg attagcccgc gcgttagcga ttaatccgga tatattatta
     6421 atggacgaag ccttctcggc gctcgatcca ttaattcgca ccgagatgca ggatgagctg
     6481 gtaaaattac aggcgaaaca tcagcgcacc attgtcttta tttcccacga tctcgatgag
     6541 gctatgcgta ttggcgaccg aattgccatt atgcaaaatg gtgaagtggt acaggtcggc
     6601 acaccggatg aaattctcaa taatccggcg aatgattatg tccgtacctt cttccgtggc
     6661 gttgatatta gtcaggtatt cagtgcgaaa gatattgccc gccggacacc gaatggctta
     6721 attcgtaaaa cccctggctt cggcccacgt tcggcactga aattattgca ggatgaagat
     6781 cgcgaatatg gctacgttat cgaacgcggt aataagtttg tcggcgcagt ttccatcgat
     6841 tcgcttaaaa ccgcgttaac gcagcagcaa ggtcttgatg cggcgctgat tgatgcgccg
     6901 ttagcagtcg atgcacaaac gcctcttagc gagttgctct ctcatgtcgg acaggcaccc
     6961 tgtgcggtgc ccgtggtcga cgaggaccaa cagtatgtcg gcatcatttc gaaaggaatg
     7021 ctgctgcgcg ctttagatcg tgagggggta aataatggct gatcaaaata atccgtggga
     7081 taccacgcca gcggcggaca gtgccgtgca atccgcagac gcctggggta caccggcgac
     7141 tgcaccgact gacggcggtg gtgctgactg gctgaccagt acgcctgcgc caaacgtcga
     7201 gcattttaat attctcgatc cgttccataa aacgctgatc ccgctcgaca gttgggtcac
     7261 tgaagggatc gactgggttg ttacccattt ccgtcccgtc ttccagggcg tgcgcgttcc
     7321 ggttgattac attctcaacg gtttccagca attgctgctg ggtatgcccg caccggtggc
     7381 gattatcgtt ttcgctctca tcgcctggca gatttccggg gtcggaatgg gcgtggcgac
     7441 gctggtttcg ctgattgcca tcggcgcaat cggtgcctgg tcgcaggcca tggttaccct
     7501 ggcgctggtg ttaaccgccc tgctgttctg tatcgtcatt ggtttgccgc tggggatctg
     7561 gctggcgaga agtccgcgag cggcgaaaat tattcgtcca ctgcttgatg ccatgcagac
     7621 cacgccagcg tttgtttatc tggtgccaat cgtcatgcta tttggtatcg gtaacgtgcc
     7681 gggcgtggtg gtgacgatca tctttgctct gccgccgatt atccgtctga ccattctggg
     7741 gattaaccag gttccggcgg atctgattga agcctcgcgc tcattcggtg ccagcccgcg
     7801 ccagatgctg ttcaaagttc agttaccgct ggcgatgccg accattatgg cgggcgttaa
     7861 ccagacgctg atgctggccc tttctatggt ggtcattgcc tcgatgattg ccgtcggcgg
     7921 gctgggtcag atggtacttc gcggtatcgg tcgtctggat atggggcttg ccaccgttgg
     7981 cggcgtcggg attgtgatcc tcgccattat cctcgaccgc ctgacgcagg ccgttgggcg
     8041 cgactcacgc agtcgcggca accgtcgctg gtacaccact ggccctgtcg gtctgctgac
     8101 ccgcccattc attaagtaac tctgcacttg cccggtaacg ccgggcatat caccctgcca
     8161 aaaaaaggaa taacaatgcg acatagcgta ctttttgcga cagcgtttgc cacgcttatc
     8221 tctacacaaa cttttgctgc cgacctgccg ggcaaaggca ttacagttaa tccagttcaa
     8281 agcaccatca ccgaagaaac cttccagaca ctgctggtca gtcgtgcgct ggagaaatta
     8341 ggttataccg tcaataaacc cagcgaagta gattacaacg ttggctacac ctcgcttgct
     8401 tccggcgatg caaccttcac cgccgtgaac tggacgccgc tgcatgacaa catgtacgaa
     8461 gctgccggtg gcgataagaa attttatcgt gaaggggtat ttgttaacgg cgcggcacag
     8521 ggttacctga tcgataagaa aaccgccgac cagtataaaa tcaccaacat cgcacaactg
     8581 aaagatccga agatcgccaa actgttcgat accaacggcg acggtaaagc ggatttaacc
     8641 ggttgtaacc ctggctgggg ctgcgaaggt gccatcaacc accagcttgc cgcgtatgaa
     8701 ctgaccaaca ccgtgacgca taatcagggg aactacgcag cgatgatggc cgacaccatc
     8761 agtcgctaca aagagggcaa accggtgttt tattacacct ggacgccgta ctgggtgagt
     8821 aatgagctga agccagggaa agatgtggtc tggttgcagg tgccgttctc cgcactgccg
     8881 ggcgataaaa atgccgatac caaactgccg aatggcgcga attacggctt cccggtcagc
     8941 accatgcata tcgttgccaa caaagcctgg accgagaaaa atccggcggc agccaaactg
     9001 tttgccatta tgcagttgcc agtggccgat attaacgccc agaacgccat tatgcatgac
     9061 ggcaaagcct cagaaggcga tattcagggc cacgttgatg gctggatcaa agcccaccag
     9121 cagcagttcg atggctgggt gaatgaggcg ctggcagcgc agaagtaatt tttattcagg
     9181 cggataaggc gtttttgcct catccgccgc tctgtacaca atgcctgatg cgacgctgac
     9241 gcgtcttatc atgcctacaa aatacactca ttcgtatggc ggataaggcg ttttcgccgc
     9301 atccgccgtt ctgtgcacaa tgcctgatgc gacgctggcg cgtcttatca tgcctacaaa
     9361 ccacatcgca catttcaaca atctatcttt catcccatat tcatcaacat ccgctattat
     9421 tgatttccag cttaatcatc acctgatgaa caaaaataat gactaaacct aatcatgagc
     9481 ttagcccggc gctgatcgtg ctgatgtcta tcgccaccgg tctggcggta gccagtaact
     9541 attacgccca gccattgctc gacaccatcg cgcgtaactt ttccctttcc gccagttcgg
     9601 caggctttat tgttaccgcc gcgcagttgg gctacgccgc aggtctactg tttcttgttc
     9661 ccctcggtga tatgtttgaa cgccgccgcc tgattgtctc gatgacctta ctggcagcgg
     9721 gcggtatgtt aatcactgcc agcagtcagt cgctggcgat gatgatcctc ggtacggcat
     9781 taaccggttt attctctgtc gtggcacaaa ttctggttcc gctggcagcg acgctggctt
     9841 caccggacaa acgcggcaaa gtggttggca ctattatgag cggtctgctg ttggggatct
     9901 tgctggcacg gacagttgcc ggattgctgg cgaatctcgg cggctggcgc accgtctttt
     9961 gggttgcctc ggtgttaatg gcactgatgg cgctggcatt atggcgtggt ctgccacaaa
    10021 tgaaatcaga aacccacctc aactacccac agttgttagg ttccgttttc agcatgttta
    10081 tcagcgataa gatcctgcgc acccgcgcat tgctgggctg cctgaccttt gccaacttca
    10141 gcattctctg gacctcaatg gcctttttgc ttgccgctcc accttttaac tacagcgatg
    10201 gtgtcattgg tctgtttgga cttgcgggag ctgccggagc gttgggcgct cgtccggcgg
    10261 gcggttttgc cgataagggt aaatcgcacc acaccacaac tttcggtctg ctgctgctat
    10321 tactttcatg gctggcgatc tggtttggtc acacttccgt actggcgctg attatcggca
    10381 tcctggtgct ggatctcacc gtacagggcg tgcatatcac taaccagacg gtaatttatc
    10441 gaatacaccc tgatgcgcgt aatcgcctga ccgcaggtta catgaccagc tactttattg
    10501 gcggtgccgc cggttcgcta atttcagcct cagcctggca acatggcggt tgggctggcg
    10561 tttgtctggc tggcgcgacg attgccctgg ttaacttact ggtctggtgg cgaggttttc
    10621 atcgtcagga agccgcaaat taagcaatca gcaaccgttt tcgtggcttt acacttataa
    10681 gggtgttaag aagcccatca gtctgataag gttaagatat tcattcagtc tatttataat
    10741 attaacaatc gttaagcgta cactctatgg aaagccctac tccacagcct gctcctggtt
    10801 cggcgacctt catggaagga tgcaaagaca gtttaccgat tgttattagt tatattccgg
    10861 tggcctttgc gttcggtctg aatgcgaccc gtctgggatt ctctcctctc gaaagcgttt
    10921 ttttctcctg catcatttat gcaggcgcga gccagttcgt cattaccgcg atgctggcag
    10981 ccgggagtag tttgtgggtt gctgcactga ccgtcatggc aatggatgtt cgccatgtgt
    11041 tgtatggccc gtcactgcgt agccgtatta ttcagcgtct gcaaaaatcg aaaaccgccc
    11101 tgtgggcatt tggcctgacg gatgaggttt ttgccgccgc aaccgcaaaa ctggtacgca
    11161 ataatcgccg ctggagcgag aactggatga tcggcattgc cttcagttca tggtcatcgt
    11221 gggtatttgg tacggtaata ggggcattct ccggcagcgg cttgctgcaa ggttatcccg
    11281 ccgttgaagc tgcattaggt tttatgcttc cggcactctt tatgagtttc ctgctcgcct
    11341 ctttccagcg caaacaatct ctttgcgtta ccgcagcgtt agttggtgcc cttgcaggcg
    11401 taacgctatt ttctattccc gtcgccattc tggcaggcat tgtctgtggc tgcctcactg
    11461 cgttaatcca ggcattctgg caaggagcgc ccgatgagct atgaggttct gctgcttggg
    11521 ttactggttg gcgtggcgaa ttattgcttc cgctatttgc cgctgcgcct gcgtgtgggt
    11581 aatgcccgcc caaccaaacg tggcgcggta ggtattttgc tcgacaccat tggcatcgcc
    11641 tcgatatgcg ctctgctggt tgtctctacc gcaccagaag tgatgcacga tacacgccgt
    11701 ttcgtgccca cgctggtcgg cttcgcggta ctgggtgcca gtttctataa aacacgcagc
    11761 attatcatcc caacactgct tagtgcgctg gcctatgggc tcgcctggaa agtgatggcg
    11821 attatataac ccacaagaat catttttcta aaacaataca tttactttat ttgtcactgt
    11881 cgttactata tcggctgaaa ttaatgaggt catacccaaa tggatagttc gtttacgccc
    11941 attgaacaaa tgctaaaatt tcgcgccagc cgccacgaag attttcctta tcaggagatc
    12001 cttctgactc gtctttgcat gcacatgcaa agcaagctgc tggagaaccg caataaaatg
    12061 ctgaaggctc aggggattaa cgagacgttg tttatggcgt tgattacgct ggagtctcag
    12121 gaaaaccaca gtattcagcc ttctgaatta agttgtgctc ttggatcatc ccgtaccaac
    12181 gcgacgcgta ttgccgatga actggaaaaa cgcggttgga tcgaacgtcg tgaaagcgat
    12241 aacgatcgcc gctgcctgca tctgcaatta acggaaaaag gtcacgagtt tttgcgcgag
    12301 gttttaccac cgcagcataa ctgcctgcat caactctggt ccgcgctcag cacaacagaa
    12361 aaagatcagc tcgagcaaat cacccgcaaa ttgctctccc gtctcgacca gatggaacaa
    12421 gacggtgtgg ttctcgaagc gatgagctaa cgcgtcatct cgctcaaaaa tccagattta
    12481 tataagaaaa aatgaccggc cagcatcgca acatgctggc ctttttggca agcaggtcgg
    12541 ctcagccgat gagttaagaa gatcgtggag aacaatatga gcgcaaatgc ggagactcaa
    12601 accccgcagc aaccggtaaa gaagagcggc aaacgtaagc gtctgctcct ccttctcacc
    12661 ttgctcttta taattattgc cgtagcgata gggatttatt ggtttttggt actgcgtcat
    12721 ttcgaagaaa ccgatgacgc atacgtggca gggaatcaga ttcaaattat gtctcaggtg
    12781 tctggcagcg tgacgaaagt ctgggccgat aacaccgatt ttgtaaaaga aggcgatgtt
    12841 ctggtcactc tcgacccgac agatgctcgc caggcgtttg aaaaagccaa aactgcactg
    12901 gcttccagcg ttcgccaaac ccaccagctg atgattaaca gcaaacagtt gcaggcgaat
    12961 attgaggtgc aaaaaatcgc cctcgcgaaa gcacaaagcg actacaaccg ccgtgtgccg
    13021 ctgggcaatg ccaacctgat tggtcgcgaa gagctgcaac acgcccgcga cgccgtcacc
    13081 agtgcccagg cgcaactgga cgtcgctatt caacaataca atgccaatca ggcgatgatt
    13141 ctggggacta aactggaaga tcagcctgcc gtgcaacagg ctgccaccga agtacgtaac
    13201 gcctggctgg cgctagagcg tactcgtatt gtcagtccga tgaccggtta tgtctcccgc
    13261 cgcgcggtac agcctggggc acaaattagc ccaacgacgc cgctgatggc ggtcgttcca
    13321 gccaccaata tgtgggtgga tgccaacttt aaagagacgc agattgccaa tatgcgtatc
    13381 ggtcagccgg tcactatcac caccgatatt tacggcgatg atgtgaaata caccggtaaa
    13441 gtagttggtc tggatatggg cacaggtagc gcgttctcac tgcttccggc gcaaaatgcg
    13501 accggtaact ggatcaaagt cgttcagcgt ctgcctgtgc gtatcgaact ggaccagaaa
    13561 cagctggagc aatacccgct gcgcattggt ttgtccacgc tggtgagcgt caataccact
    13621 aaccgtgacg gtcaggtact ggcaaataaa gtacgttcaa caccggtcgc ggtaagtacc
    13681 gcgcgtgaaa tcagcctggc acctgtcaat aaactgatcg acgatatcgt aaaagctaac
    13741 gctggctaat ccagaggtgc gtgtgatgca acagcaaaaa ccgctggaag gcgcgcaact
    13801 ggtcattatg acgattgcgc tgtcactggc gacattcatg caggtgctgg actccaccat
    13861 tgctaacgtg gcgatcccca ctatcgccgg gaatctgggc tcatcgctca gccagggaac
    13921 gtgggtaatc acttctttcg gggtggcgaa tgccatctcg atcccgctta ccggctggct
    13981 ggcaaagcgc gtcggggaag tgaaactgtt cctttggtcc accatcgcct ttgctattgc
    14041 gtcgtgggcg tgtggtgtct ccagcagcct gaatatgctg atcttcttcc gcgtgattca
    14101 ggggattgtc gccgggccgt tgatcccgct ttcgcaaagt ctattgctga ataactaccc
    14161 gccagccaaa cgctcgatcg cgctggcgtt gtggtcgatg acggtgattg tcgcgccaat
    14221 ttgcggcccg atcctcggcg gttatatcag cgataattac cactggggct ggatattctt
    14281 catcaacgtg ccgattggcg tggcggtggt gttgatgaca ctgcaaactc tgcgcggacg
    14341 tgaaacccgc accgaacggc ggcggattga tgccgtgggg ctggcactgc tggttattgg
    14401 tatcggcagc ctgcagatta tgctcgaccg cggtaaagag ctggactggt tttcatcaca
    14461 ggaaattatc attcttaccg tggtggcggt ggtggctatc tgcttcctga ttgtctggga
    14521 gctgaccgac gataacccga tagtcgatct gtcgttgttt aagtcgcgca acttcaccat
    14581 cggctgcttg tgtatcagcc tcgcgtatat gctctacttc ggcgctattg ttctgctgcc
    14641 gcagttgttg caggaggtct acggttacac ggcgacctgg gcaggtttgg cctctgcgcc
    14701 ggtagggatt attccggtga tcctgtcgcc gattatcggc cgcttcgcgc ataaactgga
    14761 tatgcggcgg ctggtaacct tcagctttat tatgtatgcc gtctgcttct actggcgtgc
    14821 ctataccttt gaaccaggta tggattttgg cgcgtcggcc tggccgcagt ttatccaggg
    14881 gtttgcggtg gcctgcttct ttatgccgct gaccaccatt acgctgtctg gtttgccacc
    14941 ggaacgactg gcggcggcat cgagcctctc taactttacg cgaacgctgg cggggtctat
    15001 cggcacgtcg ataaccacga ccatgtggac caaccgcgag tcgatgcacc atgcgcagtt
    15061 gactgagtcg gtaaacccgt tcaacccgaa tgcccaggcg atgtacagtc aactggaagg
    15121 gcttgggatg acgcaacagc aggcatcagg ctggattgcc cagcagatca ccaatcaggg
    15181 gctgattatt tccgccaatg agatcttctg gatgtcagcc ggaatattcc tcgtactgct
    15241 ggggctggtg tggtttgcta aaccgccatt tggcgcaggt ggcggcggag gcggtgcgca
    15301 ctaagtacaa ctaagccagt tcatttgaac tggctttttt caattaattg tgaagatagt
    15361 ttactgacta gatgtgcagt tcctgcaact tctctttcgg cagtgccagt tcttcgttgc
    15421 tgttgatgcg tacgtcacgt tccagaatgc tacgcgcaat atcctgcgct tcctgcaacg
    15481 agtgcatctg gtaagtgcca cactggtaga cgttcagttc cgggatctga ttctgatcct
    15541 gcactttcag cacgtcttcc attgccgctt tccaggcatc agcaacacgc tgctcatctg
    15601 gcgtaccaat cagactcata taaaaaccgg tgcggcagcc cattggcgag atatcgataa
    15661 tctctacacc cttaccgtta agatggttac gcataaaacc agcaaacagg tgctccaggg
    15721 tatggatccc tctttctggc atcacttctt tgttcggcac gcagaagcgc agatcgaaca
    15781 cggtgattgc gtcgccatgc ggggtgttca ttgttttcgc cacccgaact gcaggcgctt
    15841 ccatccgggt atgatcgact gtgaagctat ctaacaacgg catttagcca cctccggtaa
    15901 tttttttaaa aattttctga actctttctt cccaggcgag tctgagtata tgaaagacgc
    15961 gcatttgtta tcatcatccc tgaattcaga gatgaaattt tggccactca cgagtggcct
    16021 ttttcttttc tgtcaggcgt gtttttccag ccacaccgca aacggttcgg tatcagcggc
    16081 ttccatttcc tgctgacggc gttcagacgc ctcgcgctcg gctacaaaat cctcttcgcg
    16141 cagaatttcc agcggctctt cacgcagcag attacggtaa gcttctgcaa atgctttgcc
    16201 tgttccgcca ataccagtat caatcataga ccttaagata cgggcagaga aagtcagatc
    16261 gggattatcg aagcaggcaa ccagttcatc acacactttc tgatacgctt cgccgccgtt
    16321 aatactatcc agcgtttgcg cgacgcgttt cagatcgcgg aacagatctt tacccacctg
    16381 cggtaacggg aactgtgcgg tttcgcagcc gatacccagc gtcagacccg gtttgcgacc
    16441 ttcgaggatc acccggttcc agttaacgcg tgtacaggca agttcgctac tgctcatttc
    16501 cggtgcatca gccagcgcac accagaccat aaacaggtcg aggaatcgca cctgctgttc
    16561 atctacacca atcggcgaga acgggttgat gtccagcgaa cgcacttcaa tatattcaat
    16621 gccgccacgt aacagcgcat cagaaggcga ctcgccgctg cgggtaacgc gttttggacg
    16681 aatcggcgcg tacagttcgt tttcaatctg caacacgttg ctgttgattt gcagcctctt
    16741 accgtctttc tcaataccaa tcttcgcgta ctcttccgat ggcgttttga ttgcctgttt
    16801 aaggcccgct acgtactcgt aaagatcgtt gaaggtaata ccaagattgc tttgcgattt
    16861 attggtatag ccgagatcgc tcaaacgaag agaggtcgca tacggcaggt aatacatacc
    16921 gcactcggtt ttctcaaacg gcagcgacgt tggttttcct tgcaggaaag aagaacaaat
    16981 cgccggagat gcaccaaaca gataaggaat gacccaaccg aaacgatagt aattgcggat
    17041 aacgcggaaa tagcccgcag aaattttctc tttggcatca gcgcccgaga tatcaccgca
    17101 cttcgcttgc cagaatgcca ttggcaaaga gaaattgtag tgcacgccgg aaatggtttg
    17161 catcagcgcg ccgtagcgat ttttcagccc ttcacgatac agcgttttaa agcgtccggt
    17221 gttagaagtg ccgtactgtg ccagttcgat gtcctgacct tctgcgatgt agcatggcat
    17281 acttaacggc cacatccgct catcgcccat attgcgcgcc gtataacgat gcagatcgcg
    17341 cataaaggtc aacatatgtt caatatcacc atccactggt gtaatgaatt ccagcaatgc
    17401 ttccgcaaaa tcggtagtaa tccatttgtg cgtcagtgcg gaacctaatg cttcaggatg
    17461 acctgttgtt gccagtgtgc catcagcatt aacacgcaaa gtttcgcgct ccagcccacg
    17521 ctgtatcccc tttaacgcct gaggatgttt ttccagccag gccagcgcct gtgatacgtc
    17581 cgggatcaaa ttgacctccc gcctgtcaaa atcgttttaa ttagcataac tgtaatggtg
    17641 accatatgtg caggcctaca attagtgcca ccacatcatg ccctgaacgg tcgctgctgc
    17701 aactgcaaca tagcgtaacg ctttaccaag gcacaaaaaa aagattactg gcccccagga
    17761 gatgcgcatc catcccgcta acagacacag taaatcgcca accacgggca tccagcttaa
    17821 taatagcgtg actgcaccat agcgtttcag ccagccggta gctttctctt gccagcgcga
    17881 tgttttacgc aatggaaaga aacgcccaag gataacgtta gttaaccctc caaggctatt
    17941 acccattgtt gctgttaaaa ctaaaaccca gggataactg atcccggaaa gcaacattgc
    18001 caccagcacg acttcggagt tgccgggtaa tagtgtagcg ctgagaaaac tactggcaaa
    18061 caacgagaaa agcgataacg cttcactcac agcaagcgaa catccacggc gtccatgcct
    18121 gctgcacggg ccgcctgaat accgaaatcg gcatcttcaa agaccacaca ctgcgtcggt
    18181 tgcacgccca tacgctgcgc gcacaacaaa aatgtgtctg gcgcgggttt atggtgtttg
    18241 acgtgatcag cagcgacgac ggcgtcaaaa taacggcgta atcccaggtg cgccagcaat
    18301 gcctcagcga tggcgctttc actccccgtt cctacagcca ttgggcgacg accatgccaa
    18361 cttttcacca cttcaacaag aggaagcggc tcgacactat ccagcagcat acttcttact
    18421 gcttctgttt tttcacgcgc taacgcatgc gggtcgagat cggcctgatt cagctcaata
    18481 attgcctgag caatacgcca ggtgggcgat ccattaagcg caatcattgc ctgaacatcg
    18541 tactgaagac cgtagtgccc taatacttcg cgccacgctt tacggtgcgt aggctccgta
    18601 tccaggattg tgccatccat atcaaaaatt aaacctgcat aacgctcgta catggtcttc
    18661 tcgcaagtcg aaaaatatga cgctacttta gcgtaattgc ttgattttgt cgctgatggc
    18721 gaaggagaag aaaattcagg aaatggataa agtagttatt tagaaatgag atatttttga
    18781 aggaaatttt tgggagaaga tggtgcatcc gggaggatta ctcactggcg ttcgcccttc
    18841 gggccgttgc tgacgcaacg ttatcctccc tggtgcctgc ggttaatacc gctatctctt
    18901 cgataccttc attgctgaag
//
LOCUS       DAPHDW010000080        18864 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.080, whole genome shotgun sequence.
ACCESSION   DAPHDW010000080 DAPHDW010000000
VERSION     DAPHDW010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.080"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..2922
                     /gene="ehaG"
                     /locus_tag="RH057_002836"
     CDS             <1..2922
                     /gene="ehaG"
                     /locus_tag="RH057_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric autotransporter adhesin EhaG"
                     /protein_id="HDV3129180.1"
                     /translation="FTAAHGTETTSKITNVKDGDLTTGSTDAVNGSQLKTTNDAVATN
                     TTNIATNTTNISNLTETVTNLGEDALKWDKDNGVFTAAHGNNTASKITNILDGTVTAT
                     SSDAINGSQLYDLSSNIATYFGGNASVNTDGVFTGPTYKIGETNYYNVGDALAAINSS
                     FSTSLGDALLWDATAGKFSAKHGTNGDASVITDVADGEISDSSSDAVNGSQLHGVSSY
                     VVDALGGGAEVNADGTITAPTYTIANADYDNVGDALNAIDTTLDDALLWDADAGENGA
                     FSAAHGKDKTASVITNVANGAISAASSDAINGSQLYTTNKYIADALGGDAEVNADGTI
                     TAPTYTIANAEYNNVGDALDALDDNALLWDETANGGAGAYNASHDGKASIITNVANGS
                     ISEDSTDAVNGSQLNATNMMIEQNTQIINQLAGNTDATYIQENGAGINYVRTNDDGLA
                     FNDASAQGVGATAIGYNSVAKGDSSVAIGQGSYSDVDTGIALGSSSVSSRVIAKGSRD
                     TSITENGVVIGYDTTDGELLGALSIGDDGKYRQIINVADGSEAHDAVTVRQLQNAIGA
                     VATTPTKYFHANSTEEDSLAVGTDSLAMGAKTIVNGDKGIGIGYGAYVDANALNGIAI
                     GSNAQVIHVNSIAIGNGSTTTRGAQTNYTAYNMDAPQNSVGEFSVGSADGQRQITNVA
                     AGSADTDAVNVGQLKVTDAQVSQNTQSITNLDNRVTNLDSRVTNIENGIGDIVTTGST
                     KYFKTNTDGVDASAQGKDSVAIGSGSIAAADNSVALGTGSVATEENTISVGSSTNQRR
                     ITNVAAGKNDTDAVNVAQLKSSEAGGVRYDTKADGSIDYSNITLGGGNGGTTRISNVS
                     AGVNNNDAVNYAQLKQSVQETKQYTDQRMVEMDNKLSKTESKLSGGIASAMAMTGLPQ
                     AYTPGASMASIGGGTYNGESAVALGVSMVSANGRWVYKLQGSTNSQGEYSAALGAGIQ
                     W"
     gene            3291..4946
                     /gene="lldP"
                     /locus_tag="RH057_002837"
     CDS             3291..4946
                     /gene="lldP"
                     /locus_tag="RH057_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HDV3129181.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLDKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            4946..5722
                     /gene="lldR"
                     /locus_tag="RH057_002838"
     CDS             4946..5722
                     /gene="lldR"
                     /locus_tag="RH057_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HDV3129182.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHYEHSREKNA"
     gene            5719..6909
                     /gene="lldD"
                     /locus_tag="RH057_002839"
     CDS             5719..6909
                     /gene="lldD"
                     /locus_tag="RH057_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HDV3129183.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            7095..7568
                     /gene="trmL"
                     /locus_tag="RH057_002840"
     CDS             7095..7568
                     /gene="trmL"
                     /locus_tag="RH057_002840"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HDV3129184.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDFRAFLEAENPQRLFALTTKGTPAHSAVNYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(7621..8442)
                     /gene="cysE"
                     /locus_tag="RH057_002841"
     CDS             complement(7621..8442)
                     /gene="cysE"
                     /locus_tag="RH057_002841"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HDV3129185.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(8522..9541)
                     /gene="gpsA"
                     /locus_tag="RH057_002842"
     CDS             complement(8522..9541)
                     /gene="gpsA"
                     /locus_tag="RH057_002842"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HDV3129186.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(9541..10008)
                     /gene="secB"
                     /locus_tag="RH057_002843"
     CDS             complement(9541..10008)
                     /gene="secB"
                     /locus_tag="RH057_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HDV3129187.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASTQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(10071..10322)
                     /gene="grxC"
                     /locus_tag="RH057_002844"
     CDS             complement(10071..10322)
                     /gene="grxC"
                     /locus_tag="RH057_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HDV3129188.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGRTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(10463..10894)
                     /locus_tag="RH057_002845"
     CDS             complement(10463..10894)
                     /locus_tag="RH057_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HDV3129189.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVISRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKPVIVVDG
                     SGLQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            11139..12683
                     /gene="gpmM"
                     /locus_tag="RH057_002846"
                     /gene_synonym="pgmI"
     CDS             11139..12683
                     /gene="gpmM"
                     /locus_tag="RH057_002846"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HDV3129190.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLTGA
                     VDKAKNAGKAVHIMGLLSVGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            12693..13976
                     /gene="envC"
                     /locus_tag="RH057_002847"
     CDS             12693..13976
                     /gene="envC"
                     /locus_tag="RH057_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HDV3129191.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVAMQRAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRDRQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            13980..14939
                     /locus_tag="RH057_002848"
     CDS             13980..14939
                     /locus_tag="RH057_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HDV3129192.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(14926..15960)
                     /gene="waaH"
                     /locus_tag="RH057_002849"
     CDS             complement(14926..15960)
                     /gene="waaH"
                     /locus_tag="RH057_002849"
                     /EC_number="2.4.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronate:LPS(HepIII) glycosyltransferase"
                     /protein_id="HDV3129193.1"
                     /translation="MMNSTNKLSVIIPLYNAGDDFRTCMESLITQTWTALEIIIINDG
                     STDNSVEIAKHYAENYPHVRLLHQANAGASVARNRGIEVATGKYVAFVDADDEVYPTM
                     YETLMTMALEDDLDVAQCNADWCFRETGETWQSIPSDRLRSTGVLTGPDWLRMGLSSR
                     RWTHVVWMGVYRRDVIVKNNIKFIAGLHHQDIVWTTEFMFNALRARYTEQSLYKYYLH
                     NTSVSRLHRQGNKNLNYQRHYIKITRLLEKLNRNYADKITIYPEFHQQITYEALRVCH
                     AVRKEPDILTRQRMIAEIFTSGMYKRLITNVRSVKVGYQALLWSFRLWQWRDKTRSHH
                     RITRSAFNLR"
     gene            complement(16199..17224)
                     /gene="tdh"
                     /locus_tag="RH057_002850"
     CDS             complement(16199..17224)
                     /gene="tdh"
                     /locus_tag="RH057_002850"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HDV3129194.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(17234..18430)
                     /gene="kbl"
                     /locus_tag="RH057_002851"
     CDS             complement(17234..18430)
                     /gene="kbl"
                     /locus_tag="RH057_002851"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HDV3129195.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGMIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(18705..>18864)
                     /locus_tag="RH057_002852"
     CDS             complement(18705..>18864)
                     /locus_tag="RH057_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418075.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129196.1"
                     /translation="DQGIFVMCYYKRPDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQA
                     LRIFLSRK"
BASE COUNT         4615 a         5018 c         4887 g         4344 t
ORIGIN      
        1 ttcacggcag ctcatggcac cgagaccacc agcaaaatca ccaacgttaa agatggcgac
       61 ctgacgactg gcagcaccga tgccgttaac ggctctcagc tgaaaaccac caacgatgcc
      121 gtggcgacga ataccaccaa tatcgccact aacaccacca acatctctaa tctgactgag
      181 acggtgacta atcttggtga ggatgcgctg aaatgggata aggacaatgg tgtcttcact
      241 gcagctcatg gcaacaatac cgccagcaaa atcaccaata tcctggacgg cacagtcact
      301 gcaaccagtt ccgatgccat taacggtagc cagctttatg acttaagcag caatatcgcc
      361 acctacttcg gcggcaatgc ttctgtgaat actgacggtg tgtttaccgg tccaacctac
      421 aaaatcggtg aaacaaatta ttataacgtc ggcgatgcac tggctgcgat taactcctca
      481 tttagcacgt ctctcggcga tgctctgctt tgggatgcca ccgcaggtaa attcagtgcc
      541 aaacacggta ctaatggtga cgcaagcgtg atcactgatg tcgcagatgg tgaaatttca
      601 gactccagtt ctgacgcagt aaacggctca caactccacg gcgtgagcag ttatgttgtt
      661 gatgcgctgg ggggtggtgc cgaagtcaat gcagacggca ccatcactgc gccgacgtac
      721 accattgcta atgctgatta cgataatgtc ggtgatgccc tgaatgctat cgataccact
      781 cttgacgacg ctctgctctg ggatgcggac gccggtgaaa atggtgcatt tagcgccgct
      841 cacggaaaag ataaaactgc cagtgtaatc actaacgtcg ctaacggtgc aatctctgct
      901 gccagcagcg acgcgattaa cggctcacaa ctctatacca ccaataagta catcgctgat
      961 gcgctgggtg gtgacgcaga agtcaacgct gacggcacca tcaccgcacc gacttacacc
     1021 attgcgaacg ccgagtacaa caacgtcggt gacgccctgg atgcgcttga tgataacgcc
     1081 ttgctgtggg atgagactgc caatggcggt gctggagcct acaatgccag ccatgacggt
     1141 aaagccagca tcatcactaa tgtcgctaat ggcagtatta gtgaggacag taccgatgca
     1201 gtgaacggtt ctcagttgaa tgcgacgaat atgatgattg agcagaacac ccaaattatc
     1261 aatcagctcg ctggtaacac cgacgcaacc tatatccaag aaaacggtgc gggtattaac
     1321 tatgtgcgta ctaacgacga cggcttagcg ttcaacgacg ccagcgcaca gggtgttggc
     1381 gctacagcta taggttataa ctctgtcgcc aaaggcgata gcagcgtagc tattggtcag
     1441 ggcagctaca gcgacgttga tacgggtatc gccctgggta gcagctctgt ttccagccga
     1501 gtgattgcca aaggctcccg tgacaccagc ataacggaaa atggcgttgt tattggttac
     1561 gacaccacgg atggcgaact gctcggtgca ttgtctatcg gtgatgacgg taaatatcgt
     1621 caaatcatca acgtagccga tggttccgaa gcccatgacg ccgttacggt tcgtcaattg
     1681 cagaatgcga ttggtgcggt cgcaaccacg ccgactaaat acttccacgc taattcaacg
     1741 gaagaagatt cactggcagt gggaactgac tcgctggcaa tgggtgcgaa aaccatcgtg
     1801 aatggcgata aaggtattgg tatcggttat ggtgcctacg tggacgcgaa tgcacttaac
     1861 ggcattgcca ttggtagcaa tgcgcaagtc attcatgtca acagtattgc gataggtaat
     1921 ggttctacga ccactcgtgg cgctcaaacc aattataccg cctacaacat ggacgcaccg
     1981 cagaactctg tcggtgaatt ctcagtcggt agtgcggatg gtcaacgtca gatcactaac
     2041 gtcgcagcag gttcggctga taccgatgcg gtcaacgtgg gtcagttgaa agtaacggat
     2101 gcgcaggttt cccagaatac ccagagcatt actaacctgg ataatcgggt aacgaatctt
     2161 gattcacgcg tcaccaatat cgaaaacggt attggcgata tcgtcaccac cggtagcacc
     2221 aagtacttca agaccaatac cgatggtgta gatgccagcg cgcagggtaa agatagcgtc
     2281 gcgattggtt ctggctccat tgctgccgcg gataacagcg tcgcactggg tacagggtct
     2341 gtggcaaccg aagaaaatac gatctctgta ggctcatcta ctaatcaacg tcgtatcacc
     2401 aacgtagccg caggtaaaaa cgacaccgat gctgttaacg tggcacagtt gaagtcttcc
     2461 gaagctggcg gtgtgcgtta cgacaccaaa gctgatggtt ctatcgacta tagcaatatc
     2521 accctcggtg gcggcaacgg cggtacgact cgtatcagca acgtctccgc tggcgtcaac
     2581 aacaacgacg cggtgaatta cgcgcagttg aagcaaagcg tgcaggaaac gaagcaatac
     2641 accgatcagc gaatggttga aatggataac aaactgtcta aaactgaaag caagttgagc
     2701 ggtggtatcg cttctgcaat ggcaatgacc ggtctgccgc aggcttatac accgggtgcc
     2761 agcatggctt ctattggtgg cggtacttac aacggtgaat cggcagttgc tttaggtgta
     2821 tcgatggtga gcgccaatgg tcgttgggtc tacaaattac aaggtagtac caatagccag
     2881 ggtgaatact ccgccgcact cggtgccggt attcagtggt aatgatccat taagttagtg
     2941 tgactaaggg caaacgccaa attgcctgat gcgttacgcg catcaggcaa gataaggcgc
     3001 tccgggtcaa caatctaagg gcaattctct gatgaggatt gccctttttc tttaccagac
     3061 atctcccccc acaagaattg gccctaccaa ttcttcgctt atctgacctc tggttcacaa
     3121 ttccccaatt aaaactcaca tcaatattgc caatacataa catttagtta accattcatt
     3181 gtcattatcc ctacacaaca caattggcag tgccactttt acacaacgtg tgacaaggag
     3241 atgagcaaca gactcattac acgatgtgcg tggactccag gagacctgca atgaatctct
     3301 ggcaacaaaa ctacgatccc gccgggaata tctggctttc cagtctgata gcatcgcttc
     3361 ccatcctgtt tttcttcttt gcgctgatta agctcaaact gaaaggatac gtcgccgcct
     3421 cgtggacggt ggcaatcgcc cttgccgtgg ctttgctgtt ctataaaatg ccggtcgcta
     3481 acgcgctggc ctcggtggtt tatggtttct tctacgggtt gtggcccatc gcgtggatca
     3541 ttattgcagc ggtgttcgtc tataagatct cggtgaaaac cgggcagttt gacatcattc
     3601 gctcgtctat tctttcgata acccctgacc agcgtctgca aatgctgatc gtcggtttct
     3661 gtttcggcgc gttccttgaa ggagccgcag gctttggcgc accggtagca attaccgccg
     3721 cattgctggt cggcctgggt tttaaaccgc tgtacgccgc cggactgtgc ctgattgtta
     3781 acaccgcgcc agtggcattt ggcgcgatgg gcattccaat cctggttgcc ggacaagtaa
     3841 caggcatcga cagctttgag attggccaga tggtggggcg gcagctaccg tttatgacca
     3901 ttatcgtgct gttctggatc atggcgatta tggacggctg gcgcggtatc aaggagacgt
     3961 ggcctgcggt cgtggttgcg ggcggctcgt ttgccatcgc tcagtacctt agctctaact
     4021 tcattgggcc ggagctgccg gacattatct cttcgctggt atcactgctc tgcctgacgc
     4081 tgttcctcaa acgctggcag ccagtgcgcg tattccgttt tggtgatttg ggggcgtcac
     4141 aggttgatat gacgctggcc cacaccggtt acactgcggg tcaggtgtta cgtgcctgga
     4201 caccgttcct gttcctgaca gctaccgtaa cactgtggag tatcccgccg tttaaagccc
     4261 tgttcgcatc gggtggtgcg ctgtatgagt gggtgatcaa cattccggtg ccgtacctcg
     4321 ataaactggt tgcccgtatg ccaccagtgg tcagcgaggc tacagcctat gccgccgtgt
     4381 ttaagtttga ctggttctct gccaccggca ccgccattct gtttgctgca ctgctctcga
     4441 ttgtctggct gaagatgaaa ccgtctgacg ctatcagcac cttcggcagc acgctgaaag
     4501 aactggctct acccatctac tccatcggca tggtgctggc gttcgccttt atctcgaact
     4561 attccggact gtcatcaaca ctggcactgg cactggcgca caccggtcat gcattcacct
     4621 tcttctcgcc gttcctcggc tggctggggg tattcctgac cgggtcggat acctcatcta
     4681 acgccctgtt cgccgcgctg caagccaccg cagcacaaca aattggcgtc tctgatctgt
     4741 tgctggttgc cgccaatacc accggtggcg tcaccggtaa gatgatctcc ccgcaatcta
     4801 tcgctatcgc ctgtgcggcg gtaggcctgg tgggcaaaga gtctgatttg ttccgcttta
     4861 ctgtcaaaca cagcctgatc ttcacctgta tggtgggcgt gatcaccacg cttcaggctt
     4921 atgtcttaac gtggatgatt ccttaatgat tgttttaccc agacgcctgt cagacgaggt
     4981 tgccgatcgt gtgcgggcgc tgattgatga aaaaaacctg gaagcgggca tgaagttgcc
     5041 cgctgagcgc caactggcga tgcaactcgg cgtatcacgt aattcactac gcgaggcgct
     5101 ggcaaaactg gtgagcgaag gcgtgctgct cagtcgacgc ggcggcggga cgtttattcg
     5161 ctggcgtcat gacacatggt cggagcaaaa catcgtccag ccgctaaaaa cgctgatggc
     5221 cgatgatccg gattacagtt tcgatattct ggaagcccgc tacgccattg aagccagcac
     5281 cgcatggcat gcggcaatgc gcgccacacc tggcgacaaa gaaaagattc agctttgctt
     5341 tgaagcaacg ctaagtgaag acccggatat cgcctcacaa gcggacgttc gttttcatct
     5401 ggcgattgcc gaagcctcac ataacatcgt gctgctgcaa accatgcgcg gtttcttcga
     5461 tgtcctgcaa tcctcagtga agcatagccg tcagcggatg tatctggtgc caccggtttt
     5521 ttcacaactg accgaacaac atcaggctgt cattgacgcc atttttgctg gtgatgccga
     5581 cggggcgcgt aaagcaatga tggcgcacct tagttttgtt cacaccacca tgaaacgatt
     5641 cgatgaagat caggctcgcc acgcacggat tacccgcctg cccggtgagc attatgagca
     5701 ttcgagggag aaaaacgcat gattatttcc gcagccagcg attatcgcgc cgcagcgcaa
     5761 cgcattctgc cgccgttcct gttccactat atggatggtg gtgcatattc tgaatacacg
     5821 ctgcgccgca acgtggaaga tttgtcagaa gtggcactgc gccagcgtat tctgaaaaac
     5881 atgtctgact taagcctgga aacgacgctg tttaatgaga aattgtcgat gccggtagca
     5941 ctggcaccgg tgggtttgtg tggcatgtat gcgcgtcgtg gcgaagttca ggcagccaaa
     6001 gcggcggacg cgcatggcat tccgtttact ctctcgacgg tttccgtttg cccgattgaa
     6061 gaagtcgcgc cagccatcaa gcgcccaatg tggttccagc tttatgtact gcgcgatcgc
     6121 ggctttatgc gtaacgcgct ggagcgagca aaagcagcgg gttgttcgac gctggttttc
     6181 accgtggata tgccgacacc gggcgcacgc taccgtgatg cgcattcagg tatgagcggc
     6241 ccaaatgcgg caatgcgccg ctacttgcag gcggtgacgc atccgcaatg ggcgtgggat
     6301 gtgggcctga acggtcgtcc acatgattta ggtaatatct cagcttatct cggcaaaccg
     6361 accggactgg aagattacat cggctggctg gggaataact tcgatccgtc catctcatgg
     6421 aaagaccttg agtggatccg cgatttctgg gatggcccga tggtgatcaa agggatcctc
     6481 gatccggaag atgcgcgcga tgcagtacgt tttggtgctg atggaattgt ggtttctaac
     6541 cacggtggcc gccagctgga cggtgtactc tcttccgctc gtgcgttgcc cgctattgca
     6601 gatgcggtga aaggtgatat cgccattctg gcggatagcg gaattcgtaa cgggcttgat
     6661 gtcgtgcgta tgattgcgct cggtgccgac accgtacttc tgggtcgtgc tttcctgtat
     6721 gcgctggcaa cagcgggcca ggcgggtgta gctaacctgc taaatctgat cgagaaagag
     6781 atgaaagtgg caatgacgct gactggcgcg aaatcgatca gtgaaattac gcaagattcg
     6841 ctggtgcagg ggctgggtaa agagttgcct acggcactgg ctccgatggc gaaagggaat
     6901 gcggcatagt cgtttgagcc ccctcaccct aaccctctcc ctcagggagt ggggaccgtt
     6961 cggcgctgta tgtactccct cactctgaaa cgacaccgca ctcttttttt ctccctcgcc
     7021 cctccggggt gaggggaaaa ggccgcactg ctcaccaatt ttctgctatc ctgcccccgc
     7081 actaaggggg cagtatgcta aacatcgtac tttacgaacc agaaattccg ccaaatactg
     7141 gcaatatcat ccgtctttgc gctaataccg gctttcgtct gcatatcatc gaaccgatgg
     7201 gatttgcctg ggacgataag cgcctgcgcc gcgcggggct ggactatcac gagtttaccg
     7261 ccgttacgcg tcatcatgac tttcgcgcat tcctcgaagc agaaaatccc cagcgcctgt
     7321 ttgccctcac cacgaaaggt acgcctgctc acagcgccgt aaactatcag gatggcgatt
     7381 atctgatgtt cggcccggaa acacgcggcc tgccagcgag cattcttgat gccctgcccg
     7441 ctgaacaaaa aattcgcatt ccgatggtgc cggacagccg cagcatgaac ctgtccaatg
     7501 cggtgtcagt tgtggtgtat gaagcctggc ggcagctggg atatccggga gcgttgttga
     7561 gagattagtt actgtatgcc ggatagatga ttacatcgca tccggcacga tcacaggaca
     7621 ttagatccca tccccatact caaatgtatg gttaataccg ttgaaatgct ggtccatatc
     7681 cattgatggc ttatcgctgt ctggtttacc gacaatacga gccggaacgc cagcggcggt
     7741 tgtatgcggc ggcaccggtt gcagcaccac ggaacctgcg ccaatcttcg cgccgcgacc
     7801 aacttcaata ttgccgagga ttttcgcgcc cgcgccaatc atcacacctt cacgaatttt
     7861 cgggtgacgg tcaccaccag atttacccgt accgccaagc gtcacagatt gcagaatcga
     7921 tacgtcgttt tcaattaccg ccgtttcacc cacaacgatg cccgtggcgt ggtcgagcat
     7981 aatgccacgg ccaatttttg ctgccgggtg aatatcgacc tggaacgtca cagaaacctg
     8041 gttttgcaga aagattgcca atgcgcgacg cccctgattc cacaaccagt gaccgatgcg
     8101 ataggcctgc aaggcatgaa aacccttcag gtataacaac ggggttgagt atttatcgac
     8161 tgccgggtcg cgggtacgca ccgcctgaat atcacaggcc gcagaggcga tcatttccgg
     8221 gtcagcggcg taggcttctt ccaccacttc acggatagca atagcaggca taattggcga
     8281 tgacagcttg ttcgccagca tgtagctcag tgcactacca aggttttcgt gcttgagtag
     8341 cgtcgcgtgg taaaaactgg ccagcattgg ctcacagtcc gccagcgttc tggcttcggc
     8401 tttaatattg ttccagacaa tttccagttc ttcacacgac attgcttact ccacacgatg
     8461 agataatgac cggcccgttc tgcgcgggcc gggtcatagc ggtaacaaag gttccctggg
     8521 gttagtggct gctgcgctcg tccttgcgtg cacgacctaa taacgtcaat gctgcctcgc
     8581 gcgcgttttt tccgcaatat aatacttgat aaatttcctc ggttattggc atttcaacgc
     8641 cgaagcgatg cgccagttcg cggacttctt tcgtattgcg gtagccttcc accacctgac
     8701 caatcttctc ctgcgcgctt tgtacatcca tgccctgacc gagcatcatg ccaaaacggc
     8761 ggttacgcga ctggttgtcg gtacaggtaa gcaccagatc ccccagcccc gccatgccca
     8821 taaaggtggc aggatcggca cccagcgccg cgccaagacg cgacatttca gccagcccac
     8881 gggtgatcag cgccgtacgc gcattcgcac caaaaccgat accgtcggac atccccgcac
     8941 caatggcaat gacgtttttc accgcgccgc caagctgcac gccaatgaaa tccggattgc
     9001 tgtaaacgcg gaaacttttg ccgcagtgca gcagctgctg gagatcatcg gcaaaggtct
     9061 gatcggtcga ggccagcgaa atagctgtcg gtaaacctgc cgccagttct ttcgcaaacg
     9121 ttgggccaga gataaccgcc agcggaattt gatcgcctaa cgcctcgcgg gccacgtcct
     9181 gtaacagacg cccggtttcc gcttccagcc ctttggtcgc ccacaccaga cgcgcatcag
     9241 gacgcatcag cggtttaatc tggcgcagca cttcaccaaa gacatggctg ggtacgacga
     9301 cgagaatatt acggctggct gccagcgcag tggcgagatc gctttcaaga tggagcgtat
     9361 cgggaaaagg cacatcgggg agaaacgcgg cgttacagcg gtcgcgttca agcgttgcga
     9421 tatgttcagg gtcatggccc cagaggacaa cctcgtggcc atttcttgcc agggtgatgg
     9481 caagagcggt gccgtacgag ccggcaccga tcacagtcat tgaagcatta cgttggttca
     9541 tcaggcatcc tgatgttctt cagtaccttc gccagcctgc tgctgcaaat agttcatgaa
     9601 cagcgcatcg aagttaaccg gcgcaaggtt cagttgcggg aatgtaccgc gggataccat
     9661 gctggtgatg cactcacgag catacgggaa cagaatgttc gggcagtatg cgcccaggca
     9721 atgtgccatc tgggtgcctt cgatacccgc gatggagaaa ataccgccct gctgaacttc
     9781 acacaggaac gcggtttctt cgcccaggga ggccgttacg gtaacacgca gtaccacttc
     9841 gtaaacgtca tctgccagtt gagtagatgc cgtatccaga tcaagtttaa cttctggctg
     9901 ccaatctttc tggaaaacgt gcggcgcgtt cggcgcttcg aaagaaatat ccttggtata
     9961 aatacgttgg atctggaaag tcatttcggt gttgttttgt tctgacatgt gtagaaaacc
    10021 cttaagtgtt gtccttaaat actgcgcaat gtaatgccaa cgttcacacg ttatttcagc
    10081 aggggatcca gtccaccacg tgcatccaat gcatacaagt catcacagcc gccaatgtgc
    10141 tgtgcgtcaa taaaaatctg gggaaccgtg gtgcgaccgc tgcgtttgat catctcttca
    10201 cgcttggcgg cattgccatc gatcggcaac tcctggaaac tcacgccctt gctgctcagc
    10261 agtgcttttg cacgatggca atacgggcag gtttctttag tatagatttc aacattggcc
    10321 ataaattagc tcctgttttt taccctgaaa tttcatgttg cagggcggca gcaaaatgga
    10381 gtgtccccag aagcttataa caataagtcg ctgggggcct aattgcagct aacggcctgc
    10441 atcatgaaag acgacaggta aattatttgc cgcgcaccaa aggcaagttt tcgccagccc
    10501 agccagcgac gccttctttc agcacgaata cttgcgcaaa accagctttc gtcagcgcat
    10561 ttgcaggctc ctggcactgc aggccagaac cgtctaccac gataaccggt ttgtctttgt
    10621 gcttctcaag ctcaccaaca ttgttggctt tgatttcgct cggcaacaga ttgatagaac
    10681 ctgcgatatg gcctttacgg aagtcatcac gctgacgtaa atccacaacc acagcgtctt
    10741 ctttgttgat cagacgcgta gcttcaccac gactaatcac cttcactttc gaggtcaggc
    10801 tcttaaacgt agtcacaaga accgccacca gtaacgcgat ccaggcgata ctcagtatgg
    10861 gatgacggcc aacaaattgc ataatttctt gcatgggggg taacaactcc cgacgtagtg
    10921 attaaaaaaa ccaggaaagg agtatacctg cgcggtgcgg caaatacagc cagcgcgtta
    10981 actggaatgc aattttgcgg ggcgcgacga aaaaaaagcg taaatctggc atcggcttgc
    11041 cgcgagcggt cgtatatttt gatcttcaga ggctatttta tcgattcagc tgtagtaaaa
    11101 ttacgcaaat tttgactctt gagtatgagg ttgtcgcaat gtcggtttct aaaaaaccta
    11161 tggtactggt gattctggat ggctatggct atcgcgaaga acagcaggat aacgccattt
    11221 ttagtgctaa aaccccggta atggatgcac tgtgggccaa tcgtccgcat accctgatcg
    11281 acgcttccgg tctggaagtc ggtctgcctg accgtcagat gggtaactcc gaagtaggcc
    11341 acgttaacct gggtgccggc cgcatcgtgt atcaggacct gactcgtctg gacgttgaaa
    11401 tcaaagatcg cgctttcttt gctaatccag tgctgactgg cgcggtagat aaagcgaaaa
    11461 acgcaggtaa agcagtacac attatgggtc tgctctccgt aggcggcgta cacagccacg
    11521 aagatcacat catggcgatg gtagaactgg cagctgaacg cggcgcagaa aaaatctacc
    11581 tgcacgcatt ccttgacggt cgcgacaccc cgccgcgcag tgctgaatcc tcgctgaaaa
    11641 aatttgaaga gaaatttgct gcgctgggta aaggccgcgt agcgtccatc attggtcgtt
    11701 actacgcgat ggaccgcgat aaccgttggg atcgcgttga aaaagcctat gacctgctga
    11761 ctctggcgca gggtgagttc caggccgata ctgccgttgc tggtttgcag gcagcttatg
    11821 ctcgcgacga aaacgacgag ttcgtgaaag cgaccgttat tcgtgcagaa ggtcagccag
    11881 atgcggcaat ggaagacggc gatgcgctga ttttcatgaa cttccgtgct gaccgcgcgc
    11941 gtgaaatcac tcgtgcattc gtgaacgctg atttcgatgg cttcgcgcgt aagaaagtgg
    12001 ttaacgtcga tttcgtgatg ctgaccgaat acgccgctga catcaaaact gcggttgctt
    12061 acccacccgc ttccctggtt aacaccttcg gcgagtggat ggcgaaaaac gacaaaactc
    12121 agttgcgtat ttccgaaacc gaaaaatatg cccacgttac tttcttcttc aacggtggcg
    12181 tagaagagtc gttcaaaggc gaagatcgca ttctgatcaa ctcaccgaaa gtggctacct
    12241 acgatctgca accggaaatg agctccgcag agctgaccga aaaactggtt gcggcaatca
    12301 aaagcggcaa atacgacacc attatctgta actatccgaa cggcgacatg gtaggtcaca
    12361 ccggggtgat ggaagcggcg gttaaagcgg ttgaagcact ggatcactgc gtggaagaag
    12421 tcgcgaaagc ggttgagtcc gtgggtggac aactgctgat caccgctgac cacggtaacg
    12481 ctgaacagat gcgcgatccg gcaacgggtc aggcacacac ggcacacacc aacctgccag
    12541 ttccgctgat ttacgttggt gataagaacg tgaaagcggt tgcaggcggc aaactttctg
    12601 acatcgcgcc gaccatgttg tcgctgatgg gtatggaaat cccgcaagag atgactggta
    12661 agccgctgtt catcgtggaa taatccctcc ccatgagggg aaaggcgatt aataccatga
    12721 cacgggccgt gaaaccgcgc aggtttgcaa tcaggcccat catctacgcc agcgttctta
    12781 gcgctggcgt attgttgtgc gccttttccg cccacgcgga tgagcgtgac caactcaaat
    12841 ctattcaggc cgatatcgcc gcaaaagagc gcgcggtacg ccaaaagcaa caacaacgcg
    12901 caagcctgct cgcacaattg aaaaagcagg aagaagcgat ctctgaagcc acccgtaagc
    12961 tgcgcgaaac gcaaaacacg ctcaatcaac tcaataaaca gattgatgag atgaacgcgt
    13021 cgattgccaa actggagcag caaaaagccg cccaggagcg cagcctcgcc gcgcaactgg
    13081 atgccgcatt ccgtcagggc gagcataccg gtattcagct aattctcagc ggtgaagaaa
    13141 gccagcgtgg acagcgttta caggcttatt tcggctatct caaccaggcg cgacaagaaa
    13201 ccattgccca gttgaagcaa acgcgtgaag aagtcgccat gcagcgtgct gaactggaag
    13261 agaaacaaag cgagcaacaa acgctgttat atgaacagcg cgcccaacag gcgaaactga
    13321 ctcaggcgct gaacgagcgt aaaaagacgc tggcagggct ggagtcttcc atccagcaag
    13381 gtcagcaaca gttgagcgag ctgcgcgcca acgaatcccg tctgcgtaac agcattgccc
    13441 gtgcggaagc cgcggcgaaa gcgcgtgcag aacgagaagc acgtgaggcc caggcggttc
    13501 gcgaccgcca gaaagaagcg acgcgtaaag gcaccaccta caaaccgact gaaagcgaaa
    13561 aatcgctgat gtcccgtacc ggtggtctgg gcgcgccgcg tggtcaggca ttctggccgg
    13621 ttcgcgggcc gacgctgcat cgctatggcg aacagctaca gggtgaatta cgctggaaag
    13681 ggatggttat cggtgcctct gaaggtactg aagttaaagc gattgccgac ggtcgggtga
    13741 ttctggctga ctggctgcaa ggttacggtc tggtggtggt ggttgagcac ggtaaaggcg
    13801 acatgagtct ttacggctat aatcagagcg cactggtgag cgttggttcg caggttcgcg
    13861 cgggccagcc aattgcactg gtgggcagca gtggcggtca gggtcggcct tcactctatt
    13921 tcgaaattcg ccgccagggt caggcggtca atccacagcc gtggttggga agataagttt
    13981 tgtttccatt tcgtcgtaac gttcttgcat ttgccgctct gttggcgctc tcctcccccg
    14041 tacttgctgg caaacttgcc atcgtcattg atgattttgg gtatcgcccg cacaacgaaa
    14101 accaggtgct ggcgatgccc tccgctatct ccgtcgctgt attacccgat tcaccgcacg
    14161 ccagagagat ggcgaccaaa gcgcataaca gcgggcacga agtgttgatt catctcccga
    14221 tggcaccact gagtaaacag ccgctggaga aaaatacgct gcgcccggag atgagcagcg
    14281 acgaaattga gcgcattatt cgtagtgcgg tcaataacgt gccctatgcc gtggggatca
    14341 acaaccacat gggtagcaag atgacctcta acctgtttgg tatgcagaaa gtgatgcagg
    14401 cactggagcg ttacaatctt tacttcctcg acagcgtaac catcggtaat acccaggcga
    14461 tgcgcgccgc gcaaggtact ggcgtgaagg tgatcaaacg gaaggtgttc ctcgacgatt
    14521 cgcagaacga agcggacatc cgtgtgcaat ttaatcgcgc aattgatctg gcgcgtcgca
    14581 acggttcaac cattgccatt gggcatcctc acccttcaac ggtacgcgtg ctacaacaga
    14641 tggtttataa cctgccgcca gacattacgc tggtgaaagc cagcagcttg ctgaatgaac
    14701 cgcaggttga tacttctaca ccgccgaaaa acgctgtgcc tgatgcaccg cgtaatccgt
    14761 tccgtggcgt gaagctgtgc aaaccgaaga aaccgataga acctgtttat gctaatcgct
    14821 tctttgaagt attaagcgaa agcatcagcc agagcacgct gatcgtttac ttccagcatc
    14881 agtggcaagg ctggggcaaa cagcccgaag cggcgaagtt taacgctagc gcaaattaaa
    14941 ggcgctacgc gtaatgcgat ggtgcgaccg cgttttgtcg cgccattgcc ataagcggaa
    15001 agaccacagt aacgcctggt aaccgacctt cacgctgcgc acattggtaa tcaggcgctt
    15061 atacataccg gaagtaaata tctcggcaat catccgttgg cgggtaagaa tatccggctc
    15121 tttgcgcacc gcatggcaaa cacgcaatgc ttcgtaagtt atttgctgat gaaattccgg
    15181 ataaatcgta attttgtcgg cataatttcg atttaatttc tccagcaggc gggtaatctt
    15241 aatatagtga cgttgataat taaggttttt attcccttgt ctatgcaacc gactcactga
    15301 cgtattatgc agataatatt tatataatga ttgctcggta tatcgcgcac gcagcgcgtt
    15361 aaacatgaat tctgttgtcc agacaatatc ctgatgatgt aatccggcaa taaatttaat
    15421 gttattttta acaataacat cacggcgata aacccccatc cagacaacgt gagtccaacg
    15481 gcgcgaagaa agccccatcc gcagccagtc cgggccggtt aatacgccgg ttgagcgaag
    15541 gcgatcgctg gggatggatt gccaggtttc tcccgtttca cgaaaacacc agtcagcgtt
    15601 gcactgcgcc acgtcgaggt catcctctaa cgccatggtc atcagcgttt cgtacatggt
    15661 gggatagacc tcatcgtcag catcgacaaa agcgacatat ttgcccgtcg ccacctcgat
    15721 cccacgatta cgcgctaccg atgcgccagc attcgcctga tgcaacaaac gaacgtgcgg
    15781 atagttttct gcgtaatgct ttgctatttc aacagaatta tccgttgaac catcgttaat
    15841 aataatgatt tccagagcag tccaggtttg cgtaattaaa gattccatac aagtgcggaa
    15901 atcatcgccc gcattatata acggaataat aacactaagt ttattggtgc tgttcatcat
    15961 aaattaccga cgacctctga agttatcctg acgttttaca ggctttgtat taagaaacta
    16021 ttaagcctgt gagatattat ttttacaggt gagagagtca cttcagggta gagagataat
    16081 gagagcagct atataagaga tcagcgcaat tacgatatct ttattacact gacaggattt
    16141 tacatattta acggattatc ctcaggcaaa agggctggaa taccagccct tgttcgtgtt
    16201 aatcccaact cagaattact ttcccggact ggcccgaacg catagcgtca aagcccttct
    16261 ggaaatcatc gatagagaaa cgatgggtaa tgatcggcga gagatccaga cctgactgaa
    16321 tcagcgccgc catcttgtac caggtttcaa acatctcacg accgtaaata cctttaatga
    16381 acaagccttt aaagattact ttggtccagt cgatagacat atcggacggt ggaataccca
    16441 gcatcgcaat gcgaccgccg tggttcatgg tgtcgagcat ggtacgaaac gctggcggcg
    16501 cgccggacat ttccagaccg acatcaaagc cttcggtcat gcccagttct gccatcacat
    16561 cattaaggtt ttctttagcg acgttgaccg cacgggtgat acccattttg cgcgccagct
    16621 caaggcggta ttcgttaaca tcggtgatca ccacattgcg tgcaccaacg tgtttcgcca
    16681 ccgccgcagc cataatacct atcggacctg caccagaaac cagcacatct tcgcccacca
    16741 gatcgaacga cagcgcggta tgcacggcgt taccgaaggg atcaaaaatg gaagccaggt
    16801 cgtcggagat attgtcgggg attttgaaag cgttgaacgc cggaatcacc agatattcgg
    16861 cgaagcagcc aggacggtta acaccgacac caatggtgtt gcggcacaga tgggtacgac
    16921 cgccacgaca gttgcgacag tgaccgcagg tgatatggcc ttcgccagaa acgcgatcac
    16981 cgattttgaa gcctttcact tcctgaccaa tacctaccac ttcaccgaca tattcatggc
    17041 ctacgaccat cggcaccggg atggtttttt gcgaccactc atcccagtta tagatgtgaa
    17101 cgtcagtccc gcagatggct gttttacgga ttttaatcag cagatcgtta tgcccgagtt
    17161 ccggtacagg aacgtcggtc atccagatgc cctcttccgc tttcagtttg gataacgctt
    17221 tcatctcaca tcctcaggcg ataacgccca gttgtttacc aatacgcgtg aatgcttcta
    17281 ctgcacgcgt aatttgctca ggggtatgcg ccgcagacat ctgggtacga atacgcgcct
    17341 gacctttcgg aacgaccgga tagaagaaac cggtgacgta aatgccctct ttttgcagct
    17401 cacgggcaaa tttctgcgcc actactgcat cgccaagcat gaccggaata atggcatgat
    17461 cggctcccgc cagggtaaag cccgccgccg acatttgctc acggaactgg cgcgcgttcg
    17521 cccacagacg gtcacgcagt tcgctgcccg cttcgaccat ctccagtact ttgatggacg
    17581 cggcaacaat ggccggtgcc agcgagttgg agaacaggta cggacgagaa cgctggcgca
    17641 gccactcaac cacctctttg cgcgccgcgg tataaccacc agaagccccg cccagcgctt
    17701 taccaagcgt accggtgata atatcgaccc ggcccatcac atcgcagtat tcatgggaac
    17761 cacgaccatt ttcaccgaca aaaccgaccg cgtgggagtc gtctaccatc accagggcat
    17821 cgtatttatc tgccagatcg caaacgccct tcagattggc aatcatgccg tccatcgaga
    17881 atacaccatc ggtggcaatc agcacatgac gcgcaccggc ttcacgcgct tctttcagac
    17941 gagcttcaag ctcctgcata tcgttgttgg catagcgata gcgtttagct ttacacagac
    18001 gcacaccatc aataatagac gcgtggttca gtgcgtcgga gataatggcg tcttccgcgc
    18061 ccagcagcgt ttcaaacagg ccaccgttag catcaaagca ggaagagtag agaatcgcat
    18121 cttccatccc caggaaggcc gccagttttt gttcaagctc tttatggctg tcctgagtgc
    18181 cgcaaataaa acgcaccgaa gccatgccga aaccgtgaga atccattccc gcctttgccg
    18241 ccgcaatcag atcaggatga ttcgccagcc cgagatagtt gttggcacaa aagttaatga
    18301 cgtggcttcc atcagccaca gtgatatctg cttgctgcgc agacgtaata atgcgctctt
    18361 ctttaaacaa cccttccgcc cgtgcggttt ccagatcgtt ggttaactgc tgataaaatt
    18421 ctccacgcat tgcgattctc cagacagggc aaattccagc acatattacc caaacttata
    18481 ggtcgcgacg agatgacgcg ttaacacttc tgcaaaattc aggataaata acgtgtaact
    18541 tgatgtctct caacacctac agatagtaga aaaactgtac agttttggct gggcataatg
    18601 atgattgatt ttactgcatt gatataaaaa gaaaaaatga tgccaaagca tcatttttcc
    18661 tatctcagtt ggtatcaaaa cgctacccac taatggaggg aaacctattt tcttgaaagg
    18721 aaaattctca gcgcctgcat ttttgcccct aaagtgttac tcctgaagca gcgaaacaaa
    18781 tcgaaccatt ttcctctccc cagatagttt aggttgaaga gatcggggcg tttgtaatag
    18841 cacatgacaa aaatgccctg gtca
//
LOCUS       DAPHDW010000081        18781 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.081, whole genome shotgun sequence.
ACCESSION   DAPHDW010000081 DAPHDW010000000
VERSION     DAPHDW010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18781
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.081"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..117)
                     /locus_tag="RH057_002853"
     CDS             complement(<1..117)
                     /locus_tag="RH057_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001672681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="late control D family protein"
                     /protein_id="HDV3129197.1"
                     /translation="MDEQDWTITKVTHFLNNSGFTTSLELEVRLSDVEYETED"
     gene            116..1870
                     /locus_tag="RH057_002854"
     CDS             116..1870
                     /locus_tag="RH057_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001357052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase ATPase subunit family protein"
                     /protein_id="HDV3129198.1"
                     /translation="MTRLHDPRRQAALLYWQGFSVPQIAAMLQMKRPTVQSWKQRDGW
                     DSVAPISRVEMSLEARLTQLIIKPQKTGGDFKEIDLLGRQIERLARVNRYSQTGNEAD
                     LNPNVANRNKGGRRKPKKNFFSDEAIEKLEQIFFEQSFDYQLHWYRAGLEHRIRDILK
                     SRQIGATFYFSREALLRALKTGHNQIFLSASKTQAYVFREYIIAFARLVDVDLTGDPI
                     VLGNNGAKLIFLGTNSNTAQSHNGDLYVDEIFWIPNFQVLRKVASGMASQSHLRSTYF
                     STPSTLAHDAYPFWSGELFNRGRASAAERVEIDVSHNALAGGLLCADGQWRQIVTIED
                     ALKGGCTLFDIEQLKRENSADDFKNLFMCEFVDDKASVFPFEELQRCMVDTLEEWEDY
                     APFAANPFGSRPVWIGYDPSHRGDSAGCVVLAPPVVAGGKFRILERHQWKGMDFATQA
                     ESIRKLTEKYNVEYIGIDATGLGVGVFQLVRSFYPAARDIRYTPEMKTAMVLKAKDVI
                     RRGCLEYDVSATDITSSFMAIRKTMTSSGRSATYEASRSEEASHADLAWATMHALLNE
                     PLTAGISTPLTSTILEFY"
     gene            1870..2904
                     /locus_tag="RH057_002855"
     CDS             1870..2904
                     /locus_tag="RH057_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001752368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HDV3129199.1"
                     /translation="MSKKKGKTPQPAAKTMTASAPKMEAFTFGEPVPVLDRRDILDYV
                     ECISNGRWYEPPVSFTGLAKSLRAAVHHSSPIYVKRNILASTFIPHPWLSQQDFSRFV
                     LDFLVFGNAFLEKRYSTTGKVIRLETSPAKYTRRGVEEDVYWWVPSFNEPTAFTPGSV
                     FHLLEPDINQELYGLPEYLSALNSAWLNESATLFRRKYYENGAHAGYIMYVTDAVQDR
                     NDIEMLRENMVKSKGRNNFKNLFLYAPQGKADGIKIIPLSEVATKDDFFNIKKASAAD
                     LLDAHRIPFQLMGGKPENVGSLGDIEKVAKVFVRNELIPLQDRIREINGWLGQEVIRF
                     KNYSLDTDNG"
     gene            complement(3987..6263)
                     /locus_tag="RH057_002856"
     CDS             complement(3987..6263)
                     /locus_tag="RH057_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication endonuclease"
                     /protein_id="HDV3129200.1"
                     /translation="MAVKASGRFVPPSAFAAGTGKAFTGAYAWNAPREAVGRERPLTR
                     DEMRQVQGVLSTINRLPYFLRSLFTSRYDYIRRNKSPVHGFYFLTSTFQRRLWPRIER
                     VNQRHEMNTDASLLFLAERDHYARLPGMNDKELKKFAARISSQLFMMYEELSDAWVDA
                     HGEKESLFTDEAQAHLYGHVAGAARAFNISPLYWKKYRKGQMTTRQAYSAIARLFNDE
                     WWTHQLKGQRMRWHEALLIAVGEVNKDRSPYASKHAIRDVRARRQANLEFLKSCDLEN
                     RETGERIDLISKVMGSISNPEIRRMELMNTIAGIERYAAAEGDVGMFITLTAPSKYHP
                     TRQVRKGESKTVQLNHGWNDEAFNPKDAQRYLCRIWSLMRTAFKDNDLQVYGLRVVEP
                     HHDGTPHWHMMLFCNPRQRNQIIEIMRRYALKEDGDERGAARNRFQAKHLNRGGAAGY
                     IAKYISKNIDGYALDGQLDNDTGKPLKDTAAAVTAWASTWRIPQFKTVGLPTMGAYRE
                     LRKLPRGVSIADEFDERVEAARAAADSGDFALYISAQGGANVPRDCQTVRVARSPSDD
                     VNEYEEEVERVVGIYAPHLGARHIHITRTTDWRIVPKVPVVEPLTLKSGIAAPRSPVN
                     NCGKLTGGDTSLPAPTPSEHAAAVLNLVDDGVIEWSDPEVVMALRGALKHGLRTPNRQ
                     QRNGIPLKPHEIAPSARLTRSERLQITRIRVDLAQNGIRPQRWELEALARGATVNYDG
                     KKFTYPVADEWPGFSLPI"
     gene            complement(6253..6528)
                     /locus_tag="RH057_002857"
     CDS             complement(6253..6528)
                     /locus_tag="RH057_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5405 family protein"
                     /protein_id="HDV3129201.1"
                     /translation="MSIRIEIGERYVVTSDSFQFILHEKKRAESGKNAGQEWLAVVGY
                     YPKLSQLVSSLMHHDILTGSAKSFADLNAQVEQLSRRCSEAFGSYGR"
     gene            complement(6525..6608)
                     /locus_tag="RH057_002858"
                     /pseudo
     CDS             complement(6525..6608)
                     /locus_tag="RH057_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016242005.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
     gene            6597..7193
                     /locus_tag="RH057_002859"
                     /pseudo
     CDS             6597..7193
                     /locus_tag="RH057_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985242.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            complement(7380..7880)
                     /gene="cpxP"
                     /locus_tag="RH057_002860"
     CDS             complement(7380..7880)
                     /gene="cpxP"
                     /locus_tag="RH057_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HDV3129202.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            8030..8728
                     /gene="cpxR"
                     /locus_tag="RH057_002861"
     CDS             8030..8728
                     /gene="cpxR"
                     /locus_tag="RH057_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HDV3129203.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            8725..10098
                     /gene="cpxA"
                     /locus_tag="RH057_002862"
     CDS             8725..10098
                     /gene="cpxA"
                     /locus_tag="RH057_002862"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HDV3129204.1"
                     /translation="MIGSLTARIFAIFWLTLALVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(10204..10878)
                     /gene="yiiM"
                     /locus_tag="RH057_002863"
     CDS             complement(10204..10878)
                     /gene="yiiM"
                     /locus_tag="RH057_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HDV3129205.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWAREFPEQAELFVAPAFGENLSTDGLTESNVYMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
     gene            complement(11027..12010)
                     /gene="kdgT"
                     /locus_tag="RH057_002864"
     CDS             complement(11027..12010)
                     /gene="kdgT"
                     /locus_tag="RH057_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
                     /protein_id="HDV3129206.1"
                     /translation="MQIKRSIEKIPGGMMLVPLFLGALCHTFSPGAGKYFGSFTNGMI
                     TGTVPILAVWFFCMGASIKLSATGTVLRKSGTLVVTKIAVAWVVAAIASRIIPEHGVE
                     VGFFAGLSTLALVAAMDMTNGGLYASIMQQYGTKEEAGAFVLMSLESGPLMTMIILGT
                     AGIASFEPHVFVGAVLPFLVGFALGNLDPELREFFSKAVQTLIPFFAFALGNTIDLTV
                     IAQTGLLGILLGVAVIIVTGIPLIIADKLIGGGDGTAGIAASSSAGAAVATPVLIAEM
                     VPAFKPMAPAATSLVATAVIVTSILVPILTSIWSRKVKARAAKIEILGTVK"
     gene            complement(12270..12890)
                     /gene="sodA"
                     /locus_tag="RH057_002865"
     CDS             complement(12270..12890)
                     /gene="sodA"
                     /locus_tag="RH057_002865"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HDV3129207.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            13175..14137
                     /gene="rhaT"
                     /locus_tag="RH057_002866"
                     /pseudo
     CDS             13175..14137
                     /gene="rhaT"
                     /locus_tag="RH057_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(14135..14299)
                     /locus_tag="RH057_002867"
                     /pseudo
     CDS             complement(14135..14299)
                     /locus_tag="RH057_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312859.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
     gene            14587..16056
                     /gene="rhaB"
                     /locus_tag="RH057_002868"
     CDS             14587..16056
                     /gene="rhaB"
                     /locus_tag="RH057_002868"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HDV3129208.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFKNGLHS
                     QNGYVTWNVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRSNGLMAQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPEAMCSEIQAACRETAQPIPESDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIQLMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            16053..17312
                     /gene="rhaA"
                     /locus_tag="RH057_002869"
     CDS             16053..17312
                     /gene="rhaA"
                     /locus_tag="RH057_002869"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HDV3129209.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRTDLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHADDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLSWQAVWEMYC
                     QRHDTPTGSEWLESVRAYEKAILSQRG"
     gene            17554..18378
                     /gene="rhaD"
                     /locus_tag="RH057_002870"
     CDS             17554..18378
                     /gene="rhaD"
                     /locus_tag="RH057_002870"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HDV3129210.1"
                     /translation="MQNITQSWFVQGMIKATTDAWLKGWDERNGGNLTLRLDDADIAP
                     YKDNFHAQPRYIPLSQPMPLLANTPFIVTGSGKFFRNVQLDPAANLGVVKVDSDGAGY
                     HILWGLTNEAVPTSELPAHFLSHCERIKATNGKDRVIMHCHATNLIALTYVLENDTAV
                     FTRQLWEGSTECLVVFPDGVGILPWMVPGTDEIGQATAQEMQKHSLVLWPFHGVFGSG
                     STLDETFGLIDTAEKSAQVLVKVYSMGGMKQTISREELIALGKRFGVTPLASALAL"
     gene            18388..18702
                     /gene="rhaM"
                     /locus_tag="RH057_002871"
     CDS             18388..18702
                     /gene="rhaM"
                     /locus_tag="RH057_002871"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HDV3129211.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGAHNYAIY
                     LDKARNLLFATVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
BASE COUNT         4530 a         5004 c         4817 g         4430 t
ORIGIN      
        1 atcttctgtt tcgtactcca catcagaaag cctgacctca agctccaggg acgtcgtgaa
       61 gccgctatta ttcagaaaat gtgtcacctt agtgattgtc cagtcctgct cgtctatgac
      121 gcgcttacac gacccgcgtc gtcaggcggc gctgctgtac tggcaggggt tttccgtgcc
      181 gcagattgcc gccatgttgc agatgaaacg cccgacggtg cagagctgga aacagcgcga
      241 cggctgggac agcgttgccc ccatcagccg tgtcgaaatg agtctggaag cgcggctgac
      301 ccagctcatc atcaaaccgc agaaaaccgg cggtgacttc aaggaaattg acctgctggg
      361 acgccagatt gaacgactgg cacgggtaaa ccgttacagt cagaccggca acgaggcaga
      421 ccttaatccg aacgtcgcta accgcaacaa aggcggacgg cgcaaaccga aaaagaattt
      481 tttcagtgac gaggccatcg aaaagctgga gcagattttc tttgagcagt ctttcgatta
      541 tcagttgcac tggtatcgcg ccgggcttga gcaccgcatc cgcgatatcc tgaaatcccg
      601 ccagattggc gcaacgtttt atttttcccg cgaggcgctg ctgcgcgccc tgaaaaccgg
      661 tcataaccag atttttctgt cggccagtaa aacgcaggcg tatgtgttcc gcgaatacat
      721 catcgccttt gcccggctgg ttgacgttga cctgaccggt gacccgattg tcctgggcaa
      781 taacggcgca aaactgattt ttctcggcac caactccaac accgcgcaga gtcataacgg
      841 cgacctgtat gtcgacgaga ttttctggat cccgaatttt caggtactgc gtaaggtggc
      901 atcaggtatg gcctcacaga gtcacctgcg ctcgacctat ttctccaccc cgtccacgct
      961 ggcgcacgac gcctacccgt tctggtcggg tgaactgttt aaccggggac gcgccagcgc
     1021 cgctgaacgc gtggaaatcg acgtcagtca taacgccctt gccggaggtc ttctctgtgc
     1081 ggacggccag tggcggcaga ttgtcaccat tgaggacgcc ctgaaaggtg gctgcacatt
     1141 gttcgacatt gagcagctta aacgcgaaaa cagcgccgac gattttaaaa acctgttcat
     1201 gtgtgaattt gttgatgaca aggcatcggt attcccgttc gaggagctgc aacgctgcat
     1261 ggtcgacacg ctggaagaat gggaagacta tgcgccgttt gccgcaaatc cgttcggctc
     1321 ccgaccggta tggattggtt acgacccgtc acaccgtggc gacagcgccg gatgcgtggt
     1381 actggcaccg ccggtggtgg ccggtggcaa attcagaata cttgagcgtc accagtggaa
     1441 aggtatggac tttgccaccc aggcggaatc catccgcaaa ctcaccgaaa aatacaacgt
     1501 cgaatacatc ggtattgatg ccaccggcct cggtgtcggc gtgttccagc ttgtgcgctc
     1561 gttctatccc gccgcgcgcg acatccgcta cacgccggaa atgaaaaccg caatggtgct
     1621 caaggcaaaa gacgttatcc gccgtggctg tctggaatat gacgtcagcg ccaccgacat
     1681 caccagctcg ttcatggcta tccgcaagac catgaccagc agcggacgca gcgccaccta
     1741 tgaggccagc cgcagcgagg aagccagcca cgccgacctc gcctgggcga ccatgcacgc
     1801 cctgttaaat gagccactca ccgccggtat cagcactccg ctgacatcca ccattctgga
     1861 gttttactga tgagcaagaa aaaagggaaa acaccgcaac ctgcggcaaa aacaatgacc
     1921 gccagcgccc cgaaaatgga ggcattcacc tttggtgagc cggtgccggt actcgaccgc
     1981 cgtgacattc tggattacgt cgaatgcatc agtaacggca gatggtatga gccaccagtc
     2041 agctttaccg gtctggcaaa aagcctgcgt gctgccgtgc atcacagctc cccgatttac
     2101 gtcaaacgta atattctggc ttcaacgttt atcccgcacc cgtggctttc ccagcaggat
     2161 ttcagccgtt ttgtgctgga ttttctggtg ttcggtaatg cgtttctgga aaagcgttac
     2221 agcaccaccg gtaaggtcat cagactggaa acctcaccgg caaaatatac ccgccgtggc
     2281 gtggaggagg atgtttactg gtgggtgccg tccttcaacg agccgacagc cttcacgccc
     2341 ggctccgtgt ttcacctgct ggagccggat attaatcagg agctgtacgg cctgccggaa
     2401 tatctcagcg cccttaactc tgcctggctg aatgagtcgg ccacgctgtt ccgccgcaag
     2461 tattacgaaa acggcgcaca tgccggatac atcatgtacg tcaccgatgc cgtgcaggac
     2521 cgcaacgata tcgaaatgct tcgcgaaaac atggttaagt cgaaaggccg caacaacttt
     2581 aaaaacctgt ttctctatgc cccgcagggg aaagctgacg gcattaaaat tatcccgctc
     2641 agtgaagtgg caacgaagga cgattttttt aatatcaaaa aagccagtgc cgcagacctg
     2701 ctggatgcgc accgcatccc ctttcagttg atgggcggca agccggagaa cgtcgggtcg
     2761 ttgggtgata ttgagaaagt ggcaaaggtc tttgtccgca atgagcttat cccgttacag
     2821 gacaggatcc gcgagataaa cggctggctc ggtcaggagg tcatccgctt taaaaactac
     2881 tcactggaca ctgacaacgg ctaaacatcg ccgcctgcgg gcggcttttt tacaccccgt
     2941 catcacgccc tcacacactc accaccgcac aaaacagccc gcagacacac caacgccccg
     3001 gtgcacaatc taaacgccat caagacgcgc tcagacgctg aaaaaataaa atcagcacca
     3061 ccgccagcgc gcagtgcttt ccccgcctcg cccgcccgct tcacggggcg gttttaatgc
     3121 agttgcatag atactatgga tccgcaccag tcctgaccgc acgcagcctg aacggacatc
     3181 cccaacgcat gcaaaaacat tcacttgttg catgcatagc tttttaagta cgccataccg
     3241 caactgtaca tttttaagca attggcaact ttaaaaaatt tacattgctt tcaagacctt
     3301 atcatccgta gtctctgttt tttactttga gctacatcaa taaaatctca aacatgttta
     3361 atgcaaagcc cttgttacac aacatagaat gtatgtctag aaacaacgac atactatatg
     3421 ttgtgttttt ccggctctct gttcggtgat atgccaaatc actttgcgtt taaacagagc
     3481 attatttcag ggcaagactt cgctcaaaag tcattccacc taagaagcgc atataccggt
     3541 ggaagttgcc ctctacttac aggaggcaat atgaagaaat gctattactg cattctcgtt
     3601 ctggccctct ttggctatcc aaacggtagt ccgagtggtt tgtcagtaaa cgtcagtaat
     3661 atcaatgtca gcattatgct ttaatatgct tcaaacaaaa aaaccacctg ccagggtggt
     3721 ttttttgcgc ccatcatcaa tacgatgaaa gactgacaag acttcgctca agacgaatta
     3781 tgcgtcgaca taaggttacc cgcaatgatt ttattagact aaatggatgg ccttttatga
     3841 cccggatcaa acggctgtcc ttaccgacat gctagtcact caatagcatt tagccttcta
     3901 attcagcatg ctattgacct tctgctgccc cataactgct ccgcataagc cattcaatgc
     3961 catatcaaat cacgttgtgt ttttactcaa atgggtaacg agaaccccgg ccactcatca
     4021 gcaaccggat acgtgaattt tttcccgtca taatttacgg tcgcgccacg cgccagcgcc
     4081 tcaagctccc atcgttgcgg cctgataccg ttctgagcga ggtcaacgcg gatacgggta
     4141 atttgcaatc gttcagacct ggtcagtctg gcagatggtg caatttcatg cggttttaac
     4201 gggattccgt ttctttgctg acgatttggt gttctcagtc cgtgttttaa tgcgcccctg
     4261 agcgccatca cgacctccgg gtcactccat tcgataacac cgtcatctac cagattaagc
     4321 actgctgcgg cgtgctcaga aggtgtggga gccggtaacg aagtatcacc accggtgagc
     4381 tttccacagt tattgacagg actccgaggc gcggcgatgc cgctttttaa agtcaaaggc
     4441 tcaacgaccg gcactttcgg aacaatgcgc cagtccgtcg ttctggtgat atgaatatga
     4501 cgcgcgccga gatgcggcgc gtaaatgccg accactctct cgacttcttc ctcgtactcg
     4561 ttaacgtcat ccgacggact acgggcgacc ctgacagtct gacaatcgcg cgggacattt
     4621 gccccaccct gcgcgctgat atacaacgca aaatcaccac tgtctgcggc agcgcgagca
     4681 gcctcgacgc gttcgtcaaa ctcatcagca atactgacgc cgcgaggcaa tttgcgtagt
     4741 tcacggtaag cccccattgt cggcagtcca accgttttaa attgcgggat gcgccacgtt
     4801 gacgcccatg cggtaacagc cgcggcagta tctttaagcg gcttaccggt atcgttatcg
     4861 agctgaccat ccagtgcata gccgtcgata ttttttgaaa tgtatttcgc gatatatccc
     4921 gcagcaccgc cccggttaag gtgctttgcc tgaaaacggt ttcgcgcggc tcctctttcg
     4981 tcaccatcct ctttgagcgc atagcgacgc atgatttcga taatctggtt acgctggcgt
     5041 ggattacaaa aaagcatcat atgccagtgc ggcgttccgt cgtggtgtgg ctcgacgaca
     5101 cgcaaaccgt agacctgtaa atcattatcc ttgaatgccg tgcgcatcag gctccagatg
     5161 cggcagagat aacgctgcgc atcctttgga ttaaatgcct catcgttcca gccgtgatta
     5221 agctgaacgg ttttactttc gccttttctg acctgacgag tcgggtgata ctttgacggc
     5281 gcggtcagcg tgataaacat ccccacatca ccctctgcgg cggcgtaacg ctcaataccg
     5341 gcaatggtgt tcatcagctc catccggcga atttcaggat tagaaatact gcccatcacc
     5401 ttactgataa ggtcgatgcg ctcgccggtt tccctgtttt caaggtcaca cgatttaaga
     5461 aattccagat ttgcctggcg gcgcgcacgc acatcacgaa tggcgtgttt actggcataa
     5521 ggagaacggt ctttattgac ctccccgaca gctatcagta acgcttcatg ccagcgcata
     5581 cgctggcctt taagctgatg agtccaccac tcatcgttaa acagacgggc aatggcagaa
     5641 tatgcctgcc tcgtggtcat ctgcccttta cggtattttt tccagtagag cggggaaata
     5701 ttgaaagcac gtgcagcgcc agcaacatga ccatagaggt gagcctgcgc ctcatccgta
     5761 aacagcgatt ctttttcgcc atgcgcatcc acccaggcat cgctgagttc ctcatacatc
     5821 atgaaaagct gcgatgagat acgggcagca aactttttca gctccttgtc attcattccc
     5881 ggcaggcgcg catagtggtc acgctctgcc agaaacagta acgacgcgtc ggtgttcatt
     5941 tcatggcgct gattcacacg ctcaatgcgc ggccataaac gacgctgaaa agtggatgtg
     6001 aggaaataaa acccgtgcac cgggctttta ttgcgccgga tgtagtcata gcgtgaagta
     6061 aacagcgagc gcaaaaagta aggcaggcgg ttaatcgtgg ataaaacacc ttgcacctga
     6121 cgcatctcgt cacgtgtaag gggtctttcg cgcccgacgg cctcgcgtgg cgcgttccat
     6181 gcataagcac cggtaaacgc cttaccggtg cctgcagcaa atgctgacgg agggacaaaa
     6241 cgcccggagg ctttaacggc catatgagcc aaaagcctct gaacaacgcc tgctgagttg
     6301 ctcaacctgc gcgtttaaat cagcaaaaga ctttgcgctt ccggtcagaa tatcgtgatg
     6361 catcaggctg gaaacgagct ggcttaattt cggataataa ccaaccaccg ccagccattc
     6421 ctgaccggcg tttttaccgc tttccgctct ctttttctcg tggagaataa actgaaagct
     6481 gtcactggta acgacataac gttcgccaat ttcaatacga atactcatgc cgttctccgg
     6541 taatgtttgt tttttgcttc aaagactgac tggcaggaaa cacaacgcgt ggctgacgga
     6601 tatccggcat gttcacaaaa ttaattcaat tggatgaatt ttccgggcaa cacccaattc
     6661 acggactgcc gccactggcg gaggccaacc ctgaaatgac gttcctggaa aaagcagaaa
     6721 tcgaaaaact gttaaatgtt ttggctggtg atgacttact tgtcgcgctt ttatgtctga
     6781 gcactggagg aagatggacg gaagttgcca cgctaaaacc agcacagatt acaaattgca
     6841 gggttacctt cctgaaaacc aaaaacggta aaaagcgaac cgtgccgatt tctgaggaac
     6901 tggagaaaaa agttaaagag gaggccagcg ccaaattatt caaagttgat tatgagaaat
     6961 tttgcgggat tttacgcaga gtgaaacctg atataccacc caatcaggca acccacatcc
     7021 tgcggcatac attcgcaagc catttcatga tgaatggggg caatataatc gcactgcaac
     7081 agattctggg acatgcgagc attcagcaga cgatggccta tgcgcacctt gcgcctgact
     7141 atctgcaaaa tgccgtcgct ctgaatccac taaaaggcgg agtgacgtta taaatttccc
     7201 cctctgagtg tccacatagt gtccacactc aagaaatttt gaaaccgctc caaaccctta
     7261 cagctttcga taagtacctg tttttacttt tgattgcatg taagtaattg ataaaaaaaa
     7321 cccccacatc atgtggggga agacagggat ggtgtctatg gcaaggaaaa cagggtttac
     7381 tactgggaac gtgagttgct actactcaat agcttcaacg atgaactttt ttgccattgc
     7441 gtcacgtcac gcaactgctc cattcgttgt tgatgtttct cgtttaaaac cgcttgctgc
     7501 tccggcgtta acaggcgata catttggttg cggacttttg ccatctcaac ctgacgagca
     7561 atttgctcat tcgccatttt ttctgcctgt gcgcgcacag cgttttcatc aaaattttct
     7621 gcggtgacaa ggcgatgcat tgtctccagt tcgctaacat taacaggagg ctgttcgtgc
     7681 cgggcctgtt gcataagatc tcgcatctgc tgacgctgat gttcggttaa acttatgccg
     7741 tcgaacatat ggctctgcgt actgcgctgc gtaagttctt cacccggatg ccagttatcg
     7801 cctgaaccga cttcagcagc gtggcttaat gaactgactg ccagcgttga ggccatgacg
     7861 gcagcggtaa ctatgcgcat catttgctcc caaaatcttt ctgtcgcgat tcaacgatag
     7921 agagtttacg attcaggctg caaacatgcg tcagggggtg taaaacaacg taaagtcatg
     7981 gattagcgac gtctgatgac gtaatttctg cctcggaggt atttaaacaa tgaataaaat
     8041 cctgttagtt gatgatgacc gagagctgac ttccctatta aaggagctgc tcgagatgga
     8101 aggcttcaac gtgattgttg cccacgatgg ggaacaggcg cttgatcttc tggacgacag
     8161 cattgattta cttttgcttg acgtaatgat gccgaagaaa aatggtatcg acacattaaa
     8221 agcacttcgc cagacacacc agacgcctgt cattatgttg acggcgcgcg gcagtgaact
     8281 tgatcgcgtt ctcggccttg agctgggcgc agatgactat ctcccgaaac cgtttaatga
     8341 tcgtgagctg gtggcacgta ttcgcgcgat cctgcgccgt tcgcactgga gcgagcaaca
     8401 gcaaaacaac gacaacggtt caccgacact ggaagttgat gccttagtgc tgaatccagg
     8461 ccgtcaggaa gccagcttcg acggacaaac gctggagtta accggcactg agtttaccct
     8521 gctctatttg ctggcacagc atctgggtca ggtggtttcc cgtgaacatt taagccagga
     8581 agtgctgggc aaacgcctga cgcctttcga ccgcgctatc gatatgcaca tttccaacct
     8641 gcgtcgtaaa ctgccggatc gtaaagatgg tcacccgtgg tttaaaacct tgcgtggtcg
     8701 cggctatctg atggtttctg cttcatgata ggcagcttaa ccgcgcgcat cttcgccatc
     8761 ttctggctga cgctggcgct ggtgttgatg ttggttttga tgttacccaa gctcgattca
     8821 cgccagatga ccgagcttct ggatagcgaa cagcgtcagg ggctgatgat tgagcagcat
     8881 gtcgaagcgg agctggcgaa cgatccgccc aacgatttaa tgtggtggcg gcgtctgttc
     8941 cgggcgattg ataagtgggc accgccagga cagcgtttgt tattggtgac caccgaaggc
     9001 cgcgtgatcg gcgctgaacg cagcgaaatg cagatcattc gtaactttat tggtcaggcc
     9061 gataacgccg atcatccgca gaagaaaaag tatggccgcg tggaactggt cggtccgttc
     9121 tccgtgcgtg atggcgaaga taattaccaa ctttatctga ttcgtccggc cagcagttct
     9181 caatccgatt tcattaactt actgtttgac cgcccgctat tactgctgat tgtcaccatg
     9241 ttggtcagta cgccgctgct gttgtggttg gcctggagtc tggcaaaacc ggcgcgtaag
     9301 ctgaaaaacg ctgccgatga agttgcccag ggaaacttac gccagcaccc ggaactggaa
     9361 gcggggccac aggaattcct tgccgcaggt gccagtttta accagatggt caccgcgctg
     9421 gagcgcatga tgacctctca gcagcgtctg ctttctgata tctctcacga gctgcgcacc
     9481 ccgctgacgc gtctgcaact gggtacggcg ttactgcgcc gtcgtagtgg tgaaagcaag
     9541 gaactggagc gtattgaaac cgaagcgcaa cgtctggaca gcatgatcaa cgatctgttg
     9601 gtgatgtccc gtaatcagca aaaaaacgcg ctggttagcg aaaccatcaa agccaaccag
     9661 ttgtggagtg aagtgctgga taacgcggcg ttcgaagccg agcagatggg caagtcgttg
     9721 acggttaact tcccgcctgg gccgtggccg ctgtacggca atccgaacgc actggaaagt
     9781 gcgctggaaa acattgttcg taatgctctg cgttattccc atacgaagat tgaagtgggc
     9841 tttgcggtag ataaagacgg tatcaccatt acggtggacg acgatggtcc tggcgttagc
     9901 ccggaagatc gcgaacagat tttccgtccg ttctatcgga ccgatgaagc acgcgatcgt
     9961 gaatctggcg gtacaggatt ggggctggcg attgttgaaa ccgccattca gcagcatcgt
    10021 ggctgggtga aggcagaaga cagcccgctg ggcggtttac ggctggtgat ttggttgccg
    10081 ctgtataagc ggagttaaac tccgcatttg taggcaggat aaggcgttta cgccgcatcc
    10141 ggcatttgag caggatgcct gatgcgacgc tgatagcgtc ttatcaggcc tacactccgg
    10201 gggttatttt ccccacaacc ggcgggaaaa atcttcgatc ttgccgctca gtcggcgctt
    10261 ttgcatcgtc cgcgtccagc ttttcgataa cccggcggcg gagagtaaac ggtgatattg
    10321 atcgtcatca aacggcatat gccatgcgat ggcggcagcc tcctgcacgg tcacatcgct
    10381 gacacgggaa accaactcca gcggcgcgtc cgcagacact ttccctggcg ctatcacgct
    10441 atacaaccac cccaccttac ctgtgttttg catcaactgc gctatatcgc tgatatcaaa
    10501 atggtaattg agtttatagc aaggcgagcg cggctggctg acctgaatta atgcctctcc
    10561 ccagcggaaa atatcgccca tataaacatt actttccgtg agaccgtcgg ttgagagatt
    10621 ttcgccaaac gcaggcgcaa caaacaactc cgcctgttcc ggaaattccc gcgcccagta
    10681 gagataatgc tcacgaggat aatgacacag cgctctgtct ggcccgccgt gaaccttttt
    10741 ctccgcctgc tcgtcacctt ccagccccag ctccgtcagc atcaactcac catcaacctg
    10801 gattttggca attgcgctgg gtttgccttc gggataagcc tgaatcttgc ctgtgtatac
    10861 atcaaccgga tatcgcatct gttccccctc cacgagaaat ttccccatta gaccatattt
    10921 attttatgtc aggcgtgctg ttttcgcggt aagtatccag gcaggaactc aatagcaaac
    10981 cggacgaacg ttagttcatc cggctgtggg gattaaggaa gcaggtttat ttcaccgtac
    11041 ctaaaatttc gattttcgct gctctggctt tgactttacg tgaccagata gaggtaagaa
    11101 ttggcaccag aatcgaagtc acaatgaccg ccgtcgctac cagcgaagtt gctgccggag
    11161 ccatcggttt aaacgcaggc accatttctg caatcagcac aggtgtcgct accgcggccc
    11221 ctgcggaact ggaagcggca attccggcag tgccatcgcc accgccaatc aatttatcgg
    11281 cgataatcaa cggaataccg gtcacgataa ttactgccac acccaacagg atccccagca
    11341 aaccagtctg ggcaatcaca gtcaaatcaa tggtattgcc cagcgcgaag gcaaagaatg
    11401 gaatcagcgt ttgcaccgct ttgctgaaaa attctcgtaa ttcagggtca aggttcccca
    11461 gggcaaagcc caccaggaac ggtaatactg cgccgacgaa aacatgcggt tcaaacgagg
    11521 caatccctgc agtgcccaga ataatcatcg tcatgagcgg cccggactcc aacgacatca
    11581 acacaaatgc cccagcttct tcttttgtgc cgtactgctg catgatggaa gcgtaaagtc
    11641 cgccattggt catatccatc gccgccacca gcgccagcgt tgaaagtccg gcaaagaatc
    11701 caacttcaac accatgttcc ggaataatgc gcgaggcaat tgccgcaacc acccacgcga
    11761 cggcaatttt tgttaccacc agcgtaccgg atttacgcaa taccgttccc gtcgcgctta
    11821 attttattga cgcccccatg caaaaaaacc acaccgccag aatgggcacc gtaccggtaa
    11881 tcatcccgtt ggtgaatgat ccaaaatatt tccccgcccc cggcgagaag gtgtggcaca
    11941 gtgcgccaag gaatagcggg acgagcatca tcccccccgg gattttctca atcgagcgtt
    12001 ttatctgcat ttccatcacc tgtcggcata tttttaaggg ctgatgcgtc attcctcaat
    12061 ggagcatcag tgttgatgga aacgaagata gacattcatt taatcaataa aagtgatcaa
    12121 aaccacatca attgaaacac tgttttattt ttatcggatc attgttttca ttttgaaatt
    12181 gatcacaaaa aaaccaccgc cgttggcgat ggttcatttt ttaagctgat atgcggccta
    12241 tacgcctcat tgcagcaggc ggcaaatgat tattttttcg ccgcaaaacg tgccgctgct
    12301 tcgtcccagt tcaccacgtt ccagaactct ttaatgtagt ccggacggcg gttctggaat
    12361 ttcaggtagt aagcatgttc ccacacatcc aggcccagaa tcgggaagcc ggaagcgcca
    12421 gaaatagctt cacccatcag cggagaatcc tggttagcag tagaaaccac cgccagttta
    12481 tcgcctttca gcaccagcca tgcccagccg gaaccaaagc gggaagctgc cgctttttca
    12541 aattctgctt tgaagttatc aacggagccg aagtcacgtt cgatagccgc tttcaggtca
    12601 ccctgcaggg tggtgccttt tttcagacct ttccagaaca ggctgtggtt agcgtgaccg
    12661 ccagcgttgt tgcgcagtac ggttttcttg tctgctggca gctggtccag tttggtgatc
    12721 agctcttcaa ccggcaggtt ggcaaattct ggcaggcttt ccagcgccgc gttggcgttg
    12781 tttacgtagg tctgatggtg tttggtgtgg tggatttcca tggtctgctt atcgaagtgc
    12841 ggttccaggg catcgtaagc atacggcagg gatggcaggg tatagctcat attcatctcc
    12901 agtattgtcg ggcggccgat tgttaatgcc gcgtaagcag ttggttcatt atagttaatt
    12961 aaatgatatt gaaaatgatt atcaatgccg tacttttcgt aagggtatgg ttttgcagga
    13021 aaatgcccga gatgtgaagc aaatcaccca cttaatgccg tgattgccag taaatcgaca
    13081 acggcggcaa caggcgaaag gttaatcgac agcacgattt ttacactcat ctcgtcggag
    13141 atgtgaagcg acgaaaaatg atgaggataa gaagatgagt aacgcgatta cgatggggat
    13201 attttggcat ttgatcggcg cggccagtgc agcctgtttt tacgctccgt tcaaaaaagt
    13261 aaaaaaatgg tcatgggaaa ccatgtggtc agtcggtggg attgtttcgt ggattattct
    13321 gccgtgggcc atcagcgccc tgttactacc gaatttctgg acgtattaca gctcgtttag
    13381 tctctctacg ctgctgcctg tttttctgtt cggcgctatg tgggggatcg gtaatatcaa
    13441 ctatggcctg accatgcgtt atctcggcat gtcgatggga attggcatcg ccattggcat
    13501 tacgttgatt gtcggtacgc tgatgacgcc aattatcaac ggcaatttcg atgtgctgat
    13561 taacaccgaa ggcggacgca tgacgttgct cggcgttctg gtggcgctga ttggcgtagg
    13621 gattgtaact cgagccgggc agttgaaaga gcgcaagatg ggcattaaag ccgaagagtt
    13681 caatctgaaa aaagggctgg tgctggcggt gatgtgcggc attttctctg ccgggatgtc
    13741 ctttgcgata aacgccgcaa aaccgatgca tgaagccgct gccgcactag gcgtcgatcc
    13801 actgtatgtc gctctgccaa gctatgtcat catcatgggc ggcggcgcga tcatcaacct
    13861 cggtttctgt tttattcgtc tggcaaaagt gaaggatttg tcgctaaaag ccgacttctc
    13921 gctggcaaaa ccgctgatca ctcacaatgt gttactctca gcactgggcg ggttgatgtg
    13981 gtatctgcaa ttctttttct atgcctgggg ccacgcccgc attccggcgc aatatgacta
    14041 catcagttgg atgctgcata tgagcttcta tgtattgtgc ggcggtatcg tcgggctggt
    14101 gctgaaagag tggaacaatg caggacgccg tccggtaggg ctgcccatta aacacatgaa
    14161 tacccgtgcc atgttcgaca atcacaattt catgaaaatc atgatgatgt tcaggaaaat
    14221 ccgcctgcgg gagccggggt tctatcgcca cggacgcgtt accagacgga aaaaaatcca
    14281 cactatgtaa tacggtcata ctggcctcct gatgtcgtca acacggcgaa atagtaatca
    14341 cgaggtcagg ttcttacctt aaattttcga cgggaaacca cgtaaaaaac gtcgattttt
    14401 caagatacag catgaatttt caggaaatgc ggtgagcatc acaccaccac aattcagcaa
    14461 tttgtgaaca tcatcacgtt catctttccc tggttgccaa tggcccattt tcctgtcagt
    14521 agcgagaagg tcgcgaattc aggcgctttt tagactggtc gtaatgaaat tcagcaggat
    14581 cacattatga cctttcgcaa ttgtgtcgcc gtcgatctcg gcgcatccag tgggcgcgtg
    14641 atgctggcgc gttacgagcg tgaatgccgc agcctgacgc tgcgcgaaat ccatcgtttt
    14701 aaaaatgggc tgcatagcca gaacggttat gtcacctgga atgtggatag cctggaaagt
    14761 gccattcgcc ttggattaaa caaggtgtgc gaggaaggga ttcgtatcga tagcattggg
    14821 attgatacct ggggcgtgga ctttgtgctg ctcgaccaac agggtcagcg tgtgggcctg
    14881 cccgttgctt atcgcgatag ccgctccaat ggcctaatgg cgcaggcaca gcaacaactc
    14941 ggcaaacgcg atatttatca acgtagcggc atccagtttc tgcccttcaa tacgctttat
    15001 caactgcgtg cgctgacgga gcaacaacct gaacttattc cacacattgc tcacgctctg
    15061 ctgatgccgg attacttcag ctatcgcctg accggcaaga tgaactggga gtacaccaat
    15121 gccaccacca cacaactggt caatatcaat agcgacgact gggacgagtc gctactggcg
    15181 tggagcgggg ccaacaaagc ctggtttggt cgcccgacgc atccgggtaa tgtcattggg
    15241 cactggattt gcccgcaggg taatgagatt ccggtggtcg ccgttgccag ccatgatacc
    15301 gccagcgcgg ttatcgcctc gccgttaaac ggttcacgcg ccgcttatct ctcttctggc
    15361 acctggtcat tgatgggctt cgaaagccag acgccattta ccaatgacac ggcgctggca
    15421 gccaacatca ccaatgaagg cggggcggaa ggtcgctatc gggtgctgaa aaatattatg
    15481 ggcttatggc tgcttcagcg agtgcttcag gaacggcaaa tcaacgatct cccggcgctt
    15541 atcgccgcga cacaggcact tccggcctgt cgcttcatca tcaatcccaa tgacgatcgc
    15601 tttattaatc ctgaagcgat gtgcagcgaa attcaggctg cgtgtcggga aacggcgcaa
    15661 ccgatcccgg aaagtgatgc tgaactggcg cgctgtattt tcgacagtct ggcgctgctg
    15721 tatgccgatg tgttgcatga gctggcgcag ctgcgcggtg aagatttctc gcaactgcat
    15781 attgtcggcg gcggctgcca gaacacgctg ctcaaccagc tatgcgccga tgcctgcggt
    15841 attcgggtga tcgccgggcc tgttgaagcc tcaacgctcg gcaatatcgg catccagtta
    15901 atgacgctgg atgaactcaa caatgtggat gatttccgtc aggtcgtcag caccaccgcg
    15961 aatctgacca cctttacccc taatcctgac agtgaaattg cccactatgt ggcgcagatt
    16021 cactctacac gacagacaaa ggagctttgc gcatgaccac tcaactggaa caggcctggg
    16081 agctggcgaa acagcgtttc gcggcggtgg ggattgatgt cgaggaggcg ctgcgccaac
    16141 ttgatcgttt acccgtttca atgcactgct ggcagggcga tgatgtttcc ggttttgaaa
    16201 acccggaagg ttcgctgacc ggtgggattc aagctactgg taattatccg ggcaaagcgc
    16261 gtaatgccag tgagctacgt accgatctgg aacaggctat gcggctgatt ccggggccga
    16321 aacggcttaa tttacatgcc atctatctgg aatcagatac gccagtctcg cgcgatcaga
    16381 tcaaaccaga gcacttcaaa aactgggttg aatgggcgaa agccaatcag ctcggtctgg
    16441 attttaaccc ctcctgcttt tcgcatccgc taagcgccga tggctttacg ctttcccatg
    16501 ccgacgacag cattcgccag ttctggattg atcactgcaa agccagccgt cgcgtttcgg
    16561 cctattttgg cgagcaactc ggcacaccat cggtgatgaa catctggatc ccggatggta
    16621 tgaaagatat caccgttgac cgtctcgccc cgcgtcagcg tctgctggca gcactggatg
    16681 aggtgatcag cgagaagcta aaccctgcgc accatatcga cgccgttgag agcaaattgt
    16741 ttggcattgg cgcagagagc tacacggttg gctccaatga gttttacatg gggtatgcca
    16801 ccagccgcca gactgcgctg tgcctggacg ccgggcactt ccacccgact gaagtgattt
    16861 ccgacaagat ttccgccgcc atgctgtatg tgccgcagtt gctgctgcac gtcagccgtc
    16921 cggttcgctg ggacagcgat cacgtagtgc tgctggatga tgaaacccag gcaattgcca
    16981 gtgagattgt gcgtcacgat ctgtttgacc gggtgcatat cggccttgac ttcttcgatg
    17041 cctctatcaa ccgcattgcc gcgtgggtca ttggtacacg caatatgaaa aaagccctgc
    17101 tgcgtgcgtt gctggaacct accgccgagc tgcgcaagtt ggaagcggcg ggcgattaca
    17161 ctgcgcgtct ggcactgctg gaagagcaga aatcgttgtc gtggcaggcg gtctgggaaa
    17221 tgtattgcca acgtcacgat accccaacgg gtagcgaatg gctggagagc gtgcgggctt
    17281 atgagaaagc gattttgagc cagcgtgggt aaacactgcc ggatgcggca cgagcgcctt
    17341 atccggccta cgggtcgaat gtccatgcgg gctgtcaccc tcaccccggc cctctccctg
    17401 aaagggcgag ggagaaaaac atagcctcgt gcggcaacgg agctgaacac tgctccggac
    17461 agtcccctcg cccctacggg gagagggtta gggtgagggg aacaagccgc accatgtcta
    17521 aacaaggcaa cctaatttac aggaacacag aacatgcaaa acattaccca gtcctggttt
    17581 gtccagggaa tgatcaaagc caccaccgat gcctggctga aaggctggga cgagcgcaac
    17641 ggcggcaacc tgacgctgcg cctggatgac gccgatatcg cgccttataa agacaacttc
    17701 catgcacaac cgcgctatat cccgctcagc cagcccatgc ctttactggc aaatacaccg
    17761 tttattgtca ccggctccgg caaattcttc cgtaacgtcc agcttgatcc tgcagctaac
    17821 ttaggcgtcg taaaagtcga cagcgacggc gcgggctacc atattctctg ggggttaacc
    17881 aacgaagccg ttccaacttc tgaacttccg gctcactttc tctcccactg cgagcgcatt
    17941 aaagccacca acggtaaaga tcgggtgatc atgcactgcc acgccaccaa cctgatcgcc
    18001 ctcacctatg tactggaaaa cgacaccgcg gtctttactc gccaactgtg ggaaggcagc
    18061 accgagtgtc tggtggtatt cccggatggc gttggcattc tgccgtggat ggtgcccggc
    18121 acggacgaaa tcggccaggc gaccgcacaa gagatgcaaa aacattcgct ggtgttgtgg
    18181 cccttccacg gcgtcttcgg cagcgggtcg acgttggatg aaacattcgg tttaatcgac
    18241 accgcagaaa aatcagcaca agtattagtg aaggtttatt cgatgggcgg catgaaacag
    18301 accatcagcc gtgaagagtt gatagcgctc ggcaagcgct tcggcgttac accactagcc
    18361 agtgcgctgg cgctgtaagg agcaaacatg atccgcaaag cctttgtcat gcaggttaac
    18421 cccgacgccc acgaagagta tcagcgtcgg cataacccca tctggccgga actggaagca
    18481 gtgttgaaat ctcacggtgc gcataactac gccatttacc tcgacaaagc gcgtaatctg
    18541 ctgtttgcca cggtagagat tgaatctgaa gaacggtgga atgcggtagc cagtactgat
    18601 gtttgccaac gttggtggaa atatatgacc gatgttatgc ccgctaaccc ggataacagc
    18661 ccggtgagta gcgagctgca agaagtgttt tacctgccgt aattgcccaa tgtgccggag
    18721 atgcctccgg cacaatattt taaatttcga tttgaaaaat taaacatatt acatctcccg
    18781 g
//
LOCUS       DAPHDW010000082        18563 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.082, whole genome shotgun sequence.
ACCESSION   DAPHDW010000082 DAPHDW010000000
VERSION     DAPHDW010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18563
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.082"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..853
                     /locus_tag="RH057_002872"
     CDS             <1..853
                     /locus_tag="RH057_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
                     /protein_id="HDV3129212.1"
                     /translation="TITPMSPSDIIVGLYNDTIKLNLHFEWTNKNNITLSNNQTSFTS
                     GYSVTVTPAASNAKVNVSAGGGGSVMINGVATLSSASSSTRGSAAVQFLLCLLGGKSW
                     DACVNSYRNALAQNAGVYSFNLTLSYNPITTTCKPDDLLITLDSIPVSQLPATGNKAT
                     INSKQGDIILRCKNLLGQQNQTSRKMQVYLSSSDLLTNSNTILKGAEDNGVGFILESN
                     GSSVTLLNITNSSKGYTNLKEIAAKSKLTDTTVSIPITASYYVYDTNKVKSGALEATA
                     LINVKYD"
     gene            complement(935..1273)
                     /gene="rcnB"
                     /locus_tag="RH057_002873"
     CDS             complement(935..1273)
                     /gene="rcnB"
                     /locus_tag="RH057_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416610.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HDV3129213.1"
                     /translation="MTIKNKMLLGALLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(1492..2316)
                     /gene="rcnA"
                     /locus_tag="RH057_002874"
     CDS             complement(1492..2316)
                     /gene="rcnA"
                     /locus_tag="RH057_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
                     /protein_id="HDV3129214.1"
                     /translation="MTEFTTLLQQGNAWFFIPSAILLGALHGLEPGHSKTMMAAFIIA
                     IKGTIKQAVMLGLAATISHTAVVWLIAFGGMVISKRFTAQSAEPWLQLISAVIIIGTA
                     FWMFWRTWRGERNWLENMHEYDHEHHHHDHEDHHDHGHHHHHEHGEYQDAHARAHAND
                     IKRRFDGREVTNWQILLFGLTGGLIPCPAAITVLLICIQLKALTLGATLVVSFSIGLA
                     LTLVTVGVGAAISVQQVAKRWSGFNTLAKRAPYFSSLLIGLVGVYMGVHGFMGIMR"
     gene            2437..2709
                     /gene="rcnR"
                     /locus_tag="RH057_002875"
     CDS             2437..2709
                     /gene="rcnR"
                     /locus_tag="RH057_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HDV3129215.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      2801..2897
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            2932..3720
                     /gene="thiM"
                     /locus_tag="RH057_002876"
     CDS             2932..3720
                     /gene="thiM"
                     /locus_tag="RH057_002876"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HDV3129216.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTPWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRAVGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            3717..4517
                     /gene="thiD"
                     /locus_tag="RH057_002877"
     CDS             3717..4517
                     /gene="thiD"
                     /locus_tag="RH057_002877"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HDV3129217.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKSWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            4582..5400
                     /locus_tag="RH057_002878"
     CDS             4582..5400
                     /locus_tag="RH057_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HDV3129218.1"
                     /translation="MQLRITSRKKLTSLLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGIDVSRWQERIDWQRVAKMRDNGIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVAAPVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTVEELQGFVDGIKETP"
     gene            5452..6198
                     /locus_tag="RH057_002879"
     CDS             5452..6198
                     /locus_tag="RH057_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3129219.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAVEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(6172..7137)
                     /locus_tag="RH057_002880"
     CDS             complement(6172..7137)
                     /locus_tag="RH057_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3129220.1"
                     /translation="MSGARLHTLLPELTSRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     GDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGSLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFGPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(7134..8138)
                     /locus_tag="RH057_002881"
     CDS             complement(7134..8138)
                     /locus_tag="RH057_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HDV3129221.1"
                     /translation="MKTERILGALYGQALGDAMGMPSELWPRSRVKAHFGWIDRFLPG
                     PKENNAACYFNRAEFTDDTSMALCLADALLEREGKIDPDLIGRNILDWALRFDAFNKN
                     VLGPTSKIALNAIRDGKPVAELENNGVTNGAAMRVSPLGCLLPARDVDSFIDDVALAS
                     SPTHKSDLAVAGAVVIAWAISRAIDGESWSAIVDSLPSIARHAQQKRITTFSASLAAR
                     LEIALKIVRNADGTESASEQLYQVVGAGTSTIESVPCAIALVELAQTDPNRCAVLCAN
                     LGGDTDTIGAMATAICGALHGVNAIDPALKAELDAVNQLDFNRYATALAKYRQQREAV
                     "
     gene            complement(8135..9412)
                     /locus_tag="RH057_002882"
     CDS             complement(8135..9412)
                     /locus_tag="RH057_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3129222.1"
                     /translation="MKTTAKLSFMMFVEWFIWGAWFVPLWLWLSKSGFSAGEIGWSYA
                     CTAIAAILSPILVGSITDRFFSAQKVLAVLMFAGALLMYFAAQQTTFAGFFPLLLAYS
                     LTYMPTIALTNSIAFANVPDVERDFPRIRVMGTIGWIASGLACGFLPQMLGYADISPT
                     NIPLLITAGSSALLGVFAFFLPDTPPKSTGKMDIKVMLGLDALILLRDKNFLVFFFCS
                     FLFAMPLAFYYIFANGYLTEVGMKNATGWMTLGQFSEIFFMLALPFFTKRFGIKKVLL
                     LGLVTAAIRYGFFIYGSADEYFTYALLFLGILLHGVSYDFYYVTAYIYVDKKAPVHMR
                     TAAQGLITLCCQGFGSLLGYRLGGVMMEKMFAYPEPVNGLTFNWSGMWTFGAVMIAII
                     AVLFMIFFRESDNEITAIKVDDRDIALTQGEVK"
     gene            9669..10721
                     /gene="fbaB"
                     /locus_tag="RH057_002883"
     CDS             9669..10721
                     /gene="fbaB"
                     /locus_tag="RH057_002883"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HDV3129223.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLASVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            11029..11883
                     /gene="gatY"
                     /locus_tag="RH057_002884"
     CDS             11029..11883
                     /gene="gatY"
                     /locus_tag="RH057_002884"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416599.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HDV3129224.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEADALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPALDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFSQALKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
     gene            11912..13174
                     /gene="gatZ"
                     /locus_tag="RH057_002885"
     CDS             11912..13174
                     /gene="gatZ"
                     /locus_tag="RH057_002885"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HDV3129225.1"
                     /translation="MKTLIARHKAGEHIGICSVCSAHPLVIEAALAFDRNSTRKVLIE
                     ATSNQVNQFGGYTGMTPADFREFVFTIADKVGFARERIILGGDHLGPNCWQQENADAA
                     MEKSVELVKEYVRAGFSKIHLDASMSCAGDPIPLAPETVAERAAVLCFAAESVATDCQ
                     REQLSYVIGTEVPVPGGEASAIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIA
                     IVVQPGVEFDHSNIIHYQPQEAQPLAQWIESTRMVYEAHSTDYQTRTAYWELVRDHFA
                     ILKVGPALTFALREAIFALAQIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGF
                     NDSLLDIRYSLSDRIRYYWPHSRIKNSVETMMVNLEGVDIPLGMISQYLPKQFERIQS
                     GELSAIPHQLIMDKIYDVLRAYRYGCAE"
     gene            13184..13636
                     /gene="gatA"
                     /locus_tag="RH057_002886"
     CDS             13184..13636
                     /gene="gatA"
                     /locus_tag="RH057_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HDV3129226.1"
                     /translation="MTNLFVRSGISFVDRSEVLTHIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVEVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETKLKEYFTKYVLDSDE"
     gene            13667..13951
                     /gene="gatB"
                     /locus_tag="RH057_002887"
     CDS             13667..13951
                     /gene="gatB"
                     /locus_tag="RH057_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HDV3129227.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFISGVGIEALQNKILTILQG"
     gene            13955..15310
                     /locus_tag="RH057_002888"
     CDS             13955..15310
                     /locus_tag="RH057_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HDV3129228.1"
                     /translation="MFSEVMRYILDLGPTVMLPIVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDTIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRVIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQLAANAGALKAGG
                     MVASMDQGGSPITWLLIQVFSPQNIPGFIIIGAIYLTGIFMTWRRARGFTKQEKAVLA
                     E"
     gene            15358..16398
                     /gene="gatD"
                     /locus_tag="RH057_002889"
     CDS             15358..16398
                     /gene="gatD"
                     /locus_tag="RH057_002889"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV3129229.1"
                     /translation="MKSVVNDTDGVVRVAKSVIPEIKHQDEVRVKIASSGLCGSDLPR
                     IFKNGAHYYPITLGHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQ
                     CAKYDFIGSRRDGGFAEYIVVNRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCE
                     NKNVIIIGAGTIGLLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAP
                     QMQSVLRELRFNQLILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKI
                     LRKELTVIGSWMNYSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIA
                     RNAMPGKVLLIP"
     gene            16504..17277
                     /locus_tag="RH057_002890"
     CDS             16504..17277
                     /locus_tag="RH057_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HDV3129230.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSVSGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIITDDAVSEVDKLALQKTRVKLITV"
     gene            complement(17359..18258)
                     /gene="yegS"
                     /locus_tag="RH057_002891"
     CDS             complement(17359..18258)
                     /gene="yegS"
                     /locus_tag="RH057_002891"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HDV3129231.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARYVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
BASE COUNT         4772 a         4467 c         4750 g         4574 t
ORIGIN      
        1 tactatcacc cctatgagcc cgtcggatat tattgttgga ctttataacg ataccattaa
       61 attaaattta cattttgaat ggaccaataa aaacaacatc acgttatcaa ataatcagac
      121 cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg caaaagtgaa
      181 tgtttctgcg gggggcggcg gttcagtgat gattaatgga gttgcgacat tatccagtgc
      241 ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat taggtggcaa
      301 gtcatgggat gcatgtgtaa atagctacag aaatgcattg gcacaaaatg caggtgtcta
      361 ttcctttaat ctgacattgt catacaaccc gataaccaca acctgcaaac cggacgattt
      421 attaattact ttagacagta ttcccgtttc acaattacca gccacaggta acaaagcaac
      481 aataaatagt aaacaagggg atattattct gcgttgtaaa aatttattag gtcaacaaaa
      541 tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa ccaacagcaa
      601 cacaatactg aaaggtgcgg aagataatgg cgtaggattt attcttgaaa gtaatggttc
      661 gtcagtcaca cttttaaata tcactaacag cagtaaagga tacacaaatt taaaagaaat
      721 tgcggcaaag tcaaaactta cagatacaac ggtttcaatt ccgataactg ccagttacta
      781 cgtctacgat acaaacaaag ttaaatctgg cgcactggag gcaaccgcat taatcaacgt
      841 gaaatacgac taataacaag caagacgagc aagtggctaa taataaaaaa taacaaggtg
      901 tctgcatttc cccctcatga tgagggggct ttttttagcg atgataaaaa atctcaccgt
      961 cgtaggcttt aatgatttta ccgtcggtgt cgctgatcaa cacgtacgcg ccacccatat
     1021 aggtccagtg cgtcccggca tcaggcgcgg gcaggttacg taactgccac tgcttaatgt
     1081 tgtactcatc ggtccggtac atctctggga cggtatcgcc tggtttgaaa tgcttaaagt
     1141 cagcaatgaa actgcttaac tcatacttag aaattcccga ggtattggtc gaacccgcgg
     1201 tggctggtgc ggcccaggcg gcactggtaa ccagcaaaag cgcacccagc aacatcttat
     1261 ttttaatggt cataaactct ccactttggg gatctgtaat atcagtcgca tatctttcct
     1321 gccatacaat ataactgaca agcgatattt tgttagtgat cccatgattt cgtaaaaagt
     1381 gtaaatgaaa ggatgcccta aaagcatcct tagcgagcag agagtcagcg gaaatagctg
     1441 atttaagaat catcctgggc tgaagataac atgcaagcct aaaggattga gttatcgcat
     1501 tatgcccatg aagccatgta cgcccatata cacaccgact aagccaatca acagactgga
     1561 aaaatagggg gcgcgtttag cgagagtgtt aaatccgctc cagcgttttg cgacctgttg
     1621 aacgctgatt gctgcgccaa cgcctacggt gacaagcgtt aacgccaggc caatgctgaa
     1681 actgacgacc agtgttgcgc ccagtgtcag ggctttcaac tgaatgcata tcaacagcac
     1741 ggtaattgct gccgggcagg ggataaggcc accggttaag ccaaataaca aaatttgcca
     1801 gttggtgacc tctctaccat caaagcgtcg tttaatgtca ttggcatggg ctcgtgcatg
     1861 ggcatcctga tactcgccat gttcgtgatg gtgatgatgt ccatggtcgt ggtgatcttc
     1921 gtgatcgtgg tgatgatgtt catgatcata ctcgtgcata ttctccagcc agttgcgttc
     1981 gccgcgccag gtacgccaga acatccagaa cgcggtgcca ataatgatca ctgcggaaat
     2041 cagctggagc cacggttctg ctgattgagc agtaaagcgc ttgctgatca ccatcccgcc
     2101 aaaggcaatt aaccagacca ctgcggtatg cgaaatagtt gctgccagtc cgagcatcac
     2161 cgcttgttta atggtgcctt tgatggcgat gataaacgcc gccatcatcg tttttgagtg
     2221 ccctggttcc aggccatgaa gcgcaccaag taagatggcg ctggggatga agaaccaagc
     2281 gtttccttgc tgaagaagag ttgtaaattc ggtcatgata ataattctta gtattaattc
     2341 ggcaatctga ttctactccc ccccagtacc tgatactacc ccccagtaga ttaatagtgc
     2401 tatgattttt catattcttg ttaaccaggt gttgccatgt ctcatacaat ccgtgataaa
     2461 cagaaactga aagcgcgtgc cagtaagatt cagggccagg tcgtggcgct caagaaaatg
     2521 ctcgacgagc cgcacgaatg cgctgcagtt ttacaacaga ttgctgctat ccgtggcgcg
     2581 gtaaacggtc tgatgcggga agtgattaaa ggtcatctga cggaacacat cgttcaccag
     2641 ggggatgagc taaaacgtga agaagatctg gatgtcgttc tgaaggtgct ggattcatat
     2701 atcaaataat ttattaacgc gattgtaaaa ctgccgtttt tcctcgttta caacgcgtgc
     2761 gctggacatt accatcctcc tctgcgattt atcatcgcaa ccaaacgact cggggtgccc
     2821 ttctgcgtga aggctgagaa atacccgtat cacctgatct ggataatgcc agcgtaggga
     2881 agtcacggac caccaggtca ttgcttcttc acgttatggc aggagcaaac tatgcaagtc
     2941 gacctgctga gttcagcgca atctgcgcac gcgttacacc tttttcacca acattcccct
     3001 cttgtgcact gcatgaccaa cgatgtggtg caaaccttta ctgccaatac cttgctggcg
     3061 ctcggtgcat cgccagcgat ggttatcgaa accgaagagg ccagtcagtt tgcggctatc
     3121 gccagtgcct tgttgattaa cgttggcaca ctgacgcagc cacgcgctca ggcgatgcgt
     3181 gctgccgttg agcaagcaaa aagctctcaa acaccctgga cgcttgatcc agtagcggtt
     3241 ggtgcgctcg attatcgccg ccatttttgt catgaacttt tatcttttaa accggcagcg
     3301 atacgtggta atgcttcgga aatcatggca ttagctggca ttgctaatgg tggacgggga
     3361 gtggatacca ctgacgccgc agctaacgcg atacccgctg cacaaacact ggcacgggaa
     3421 actggcgcaa tcgtcgtggt cactggcgag gtggattatg ttaccgatgg ccatcgcgcc
     3481 gttggcattc acggtggcga tccgttaatg accaaagtgg taggaactgg ctgtgcatta
     3541 tcggcggttg tcgctgcctg ctgtgcgtta ccaggcgata cgctggaaaa tgtcgcatct
     3601 gcctgtcact ggatgaaaca agccggagag cgcgcagtcg ccagaagcga ggggccaggc
     3661 agttttgttc cacatttcct tgatgcgctc tggcaattga cgcaggaggt gcaggcatga
     3721 aacgaattaa cgctctgacg attgccggta ctgatcccag tggtggtgcg gggattcagg
     3781 ccgatcttaa aaccttctcg gcacttggcg cttatggttg ctcagttatt actgcactgg
     3841 tggcgcaaaa tacccgtggc gtacagtcgg tgtatcgcat tgagcctgat tttgtcgccg
     3901 cccagctcga ttcggtgttc agcgatgtgc gaatcgacac cactaaaatc ggtatgttgg
     3961 cggaaaccga tattgttgaa gcggtggcag aacggttgca acgttatcag atccaaaacg
     4021 tggtactcga caccgttatg ctggcaaaaa gcggcgaccc gctgctttca ccttcggcgg
     4081 ttgctacgct gcgcagtcga ttattgccac aggtttcatt aataacgcca aacttgcccg
     4141 aagctgccgc cttgctcgac gcgccacacg cgcgcaccga acaggaaatg ctggaacaag
     4201 ggcgatcgct gttggcgatg ggctgtggcg cagtgctaat gaaaggtggt catctggatg
     4261 atgagcaaag cccggactgg ctgtttaccc gcgagggtga acaacggttt accgcaccgc
     4321 gcattatgac caaaaacacc cacggcactg gttgtacgct ctctgcggcg ttggctgcac
     4381 tacgcccgcg ccatacaaac tgggctgaca ccgtacagga ggcaaaaagc tggctttcat
     4441 cagcattagc ccaggccgac acgctggaag ttggtcacgg tattggtccg gttcaccact
     4501 tccacgcctg gtggtgagca ccgtactata gctgctttgg ttattgtcag cctttggcac
     4561 tgacattcag ggatgaaacc aatgcagcta agaattacca gtcgaaaaaa actcacctct
     4621 ctattatgcg ctcttgggct aatttccatc gttgcaattt atccgcgtca gacggtgaat
     4681 tttttctatt cgacagcagt tcagattaca gactacatcc atttctacgg ttatcgcccg
     4741 gttaaatctt tcgctattcg cattcctgcc agttacacca ttcacggaat agatgtttct
     4801 cgctggcagg agcggatcga ctggcagcgg gtggcaaaaa tgcgcgacaa cggtatccgc
     4861 ttacagtttg cttttattaa ggcgacggaa ggcgaaaagc tggtggaccc gtatttttcg
     4921 cgtaactggc aactaagccg cgaaaatggc ctgctgcgcg gggcgtatca ttatttctcc
     4981 ccgtcggtag cagcgccggt tcaggcacga ttatttctgc aaacggtgga tttctcacaa
     5041 ggcgatttcc ctgccgtgct ggacgtagaa gaacggggaa aattatcggc aaaagaatta
     5101 cgcaagcggg taagtcagtg gctaaaaatg gtagaaaaac gcacgggaaa aaagccgatt
     5161 atttactcag gagccgtttt ttatcacacg aatctggcgg gctattttaa tgaatatccg
     5221 tggtgggtgg ctcactatta tcaacgtcgt ccggacaatg acggaatggc ctggcgcttc
     5281 tggcagcatt ccgaccgtgg acaggtagat ggcatcaatg gtccggtgga ttttaatgtg
     5341 tttaatggca cggtggagga gttgcaggga ttcgttgatg ggattaaaga aacgccttaa
     5401 ataagaagca gtgatatact cccaccagac tccctggcag gaaaaagaca aatggaacaa
     5461 gcgcataccc agctgattgc ccaactgaac gagcgtattt tagcggcgga taacacgccg
     5521 ctttatatta agtttgccga aacggtaaaa aatgccgtgc gcagcggggt gctggagcat
     5581 ggcaatattt tgcccggtga gcgtgattta agtcagttaa ccggcgtttc gcgcattacg
     5641 gtgcgcaagg cgatgcaggc gctggaagaa gagggtgtgg tgacgcgttc gcgcggttac
     5701 ggcacgcaga tcaacaacat cttcgaatac tcgttgaaag aagcgcgagg tttttctcag
     5761 caggtggtat tgcgcgggaa aaagcccgat acgctatggg ttaacaagcg tgtcgtgaaa
     5821 tgtcccgaag aagtcgcgca gcagctggcg gtcgaagcag gaagtgatgt ctttttgctt
     5881 aagcgtattc gctatgtcga tgaagaagcg gtatcgattg aggaatcgtg ggttccggcg
     5941 catttaattc atgatgttga tgccatcggg atttcacttt atgactattt ccgcagccag
     6001 cacatttacc cacaacgtac gcgttcccgc gttagcgccc ggatgccgga tgccgagttt
     6061 cagtcacata ttcagttaga tagcaaaata ccggtgctgg tgatcaagca agtggcgctt
     6121 gaccaacagc aacggcctat tgagtacagc attagccact gtcgcagcga tctatacgtt
     6181 tttgtgtgcg aggagtagtt cctcgcgcgt tggcgcacaa tcaccgcccc gatgcccgac
     6241 aacccacgac gccactgcat tgcccagtaa tacggcatcc gccagtggca gaccagaggc
     6301 cagcccggca agtacgccac ctgcatgact gtcgcccgcc ccaatggtgt ctacaacttg
     6361 cgttggaaat gccggaatgc agccagaagc gtcgttgctg aaataccatg caccctcttt
     6421 atcgagacga acgatcaacg gcgcggcaaa tttctcctgc cattgcttgc caagtgttgt
     6481 tatctctgcg gataaagcaa aacgttcggc ggcaatctca gcctcttgac gattgagtga
     6541 tactaaaggt cgacaggcca tgatccgcgc cagtaatgcg tccgggatat cgccgatacg
     6601 tgggccaaaa tcgataaacg gcgtcacgtc ttgcagcttt tccagccatt ccactaacaa
     6661 ttcgccgcag ggcgaggcca gttgataacc ggaaaaatag agcaggctgc caggcgcaac
     6721 ggttaatcgc gccagccact ggcgattcca ctgattttcc acaccgctga atgacataaa
     6781 agtgcgttcg ccatccggct caaccagcgc cagacaccag ccgttatcgc cttcggcgtt
     6841 atcgatcagg ctgattaacc cctctttcgc catccggttg cgaataatct ccgcccacac
     6901 gccctgaccg agcggcaagg cattacccgc ttcgatgccg aggcgcttta acgccacggc
     6961 aatattcagt gcgcagccgc caacgttaac gctctgctgc ttcagttcga tatcgcaccc
     7021 acgccagggg agggcataag cgtcggcgat cacatcaatg accgccgcgc cgacgaccat
     7081 caccggctga cgcgaggtta attcaggcag cagcgtgtgt aatcgagcgc cgctcatacc
     7141 gcctcccgtt gttgacgata tttcgccagc gctgtggcat agcggttgaa atcaagctga
     7201 tttaccgcat ccagttccgc ctttaatgca ggatcgatag cgttaacgcc atgcaacgcg
     7261 ccgcaaatcg ccgtcgccat agcgccgatg gtgtctgtgt cgccgccaag gttagcgcac
     7321 aggacggcgc agcgattcgg gtcggtttgt gccagttcaa ccagcgcaat ggcgcacgga
     7381 acggactcaa tagtgctggt acctgcgcca acgacctggt aaagctgttc gctggcggat
     7441 tcggtgccgt cggcattgcg cacaattttc agcgcaatct ccagacgtgc tgccagtgag
     7501 gcgctgaagg tagtgatgcg tttttgctgt gcatgtcgcg caattgaagg cagggaatca
     7561 acaatcgctg accagctttc tccgtcaatg gcacgagaaa tcgcccatgc gatgactacc
     7621 gcacctgcaa ctgccagatc ggatttatgg gtcgggctcg acgccagcgc cacatcatca
     7681 ataaaggaat caacatcacg cgccggaagc aaacaaccta atggcgagac gcgcatcgct
     7741 gcgccgttgg tcacgccgtt gttttccagt tcagcaacgg gtttaccgtc gcgaatggcg
     7801 ttaagcgcaa tcttcgaggt cggacctagt acgtttttgt taaaggcgtc gaaacgcagc
     7861 gcccagtcga gaatattacg cccaatcaga tccggatcga tcttgccttc acgttccagt
     7921 aacgcatccg ccagacacag cgccatcgag gtatcgtcgg tgaattcggc gcggttaaaa
     7981 taacaggccg cgttattctc ctttggtcca ggcagaaaac ggtcaatcca gccaaagtgt
     8041 gctttaacgc ggctgcgtgg ccaaagctcg gagggcatcc ccatcgcatc ccctaacgcc
     8101 tgcccataaa gagcaccgag aatacgttct gttttcattt aacttcccct tgtgtcaacg
     8161 caatatcgcg atcatcgacc ttgatagccg taatttcgtt gtcggattcg cggaaaaaaa
     8221 tcatgaacag cacggcgata atggcaatca tcaccgcgcc gaaagtccac atcccggacc
     8281 agttgaaagt cagcccgttt accggttccg gataagcgaa catcttttcc atcatcacac
     8341 cgccaagacg atagccgagc aaactgccga agccctggca gcagagcgtg atcagcccct
     8401 gcgcggcggt acgcatatgc acgggggctt ttttatcgac atagatgtaa gcggtaacgt
     8461 agtaaaaatc gtaacttacg ccgtgaagca aaataccgag gaacagtaac gcgtaggtga
     8521 aatattcatc cgcactaccg taaataaaga agccatagcg gatcgcagcg gtgaccagac
     8581 caagcaataa tacctttttg ataccaaagc gtttagtgaa aaacggcaat gccagcataa
     8641 agaagatttc agagaactgg ccgagcgtca tccagccggt ggcgtttttc atgccaactt
     8701 cggtcagata accgttggca aagatgtaat agaacgccag tggcatcgca aacaggaatg
     8761 aacagaagaa aaagacgagg aagtttttat cgcgcagcag gatcagcgca tccaggccga
     8821 gcatgacttt aatgtccatt ttgccggtgc tttttggcgg cgtgtcgggc aggaaaaacg
     8881 caaacacacc gagcagagca gaacttccgg cggtaatcag cagcgggatg ttagtcggtg
     8941 agatatcggc ataccccagc atttgcggca agaaaccaca tgccagacca gaggcaatcc
     9001 agccgatagt gcccatcaca cgaatgcgcg ggaaatcacg ctccacatcc ggcacgttgg
     9061 caaaagcgat actgttagtc agcgcaatgg tcggcatata ggttagagag taagccagca
     9121 gtaacggaaa gaacccggca aaagtggtct gttgcgcagc gaaatacatc agcagcgcgc
     9181 ctgcgaacat caataccgcc agcacttttt gcgccgagaa aaagcggtca gtgatggagc
     9241 caaccagaat tggcgatagg atcgccgcaa tggcggtaca ggcatacgac cagccaattt
     9301 ctccggcact aaaaccgctt ttacttaacc acaaccacaa tggcacaaac cacgcgcccc
     9361 agataaacca ttcaacaaac atcatgaacg acagctttgc tgttgttttc atcttttaat
     9421 tccttcatga caggtaatgt aaggtacgcg atgacaatac cacttaataa taccttttaa
     9481 atacctttgc ggtgaaaact tgaccgcgct aacatttttt ctgatgaatc gagccaacag
     9541 aaaacgctga aaaaacatcc aaaagatgga aaaactcgcc cgtaacgacc aggattatga
     9601 ttgcccgcaa aaggcgggcc aggacaatcc atagccgata tccaatcgga atttacggga
     9661 gcatagtaat gacagatatt gcgcagttgc ttggcaaaga cgccgacaac cttttacagc
     9721 accgttgtat gacaattcct tctgaccagc tttatctccc cggacatgac tacgtagacc
     9781 gcgtaatgat tgacaataat cgcccgccag cggtgttacg taatatgcag acgttgtaca
     9841 acaccgggcg tctggctggc acaggatatc tttctattct gccggttgac cagggcgttg
     9901 agcactctgc cggagcttca tttgctgcta acccgctcta ctttgacccg aaaaacattg
     9961 ttgaactggc gatcgaagcg ggctgtaact gtgtggcgtc aacttacggc gtgctggcgt
    10021 cggtatcgcg gcgttatgcg catcgcattc cattcctcgt caaacttaat cacaacgaga
    10081 cgctaagtta cccgaatacc tacgatcaaa cgctgtatgc cagcgtggag caggcgttca
    10141 acatgggcgc ggttgcggtt ggtgcgacta tctattttgg ctcggaagag tcacgtcgcc
    10201 agattgaaga aatttctgcg gcttttgaac gtgcgcacga gctgggtatg gtgacagtgc
    10261 tgtgggccta tttgcgtaac tccgccttta agaaagatgg cgttgattac catgtttccg
    10321 ccgacctgac cggtcaggca aaccatctgg cggcaaccat cggtgcagat atcgtcaaac
    10381 aaaaaatggc ggaaaataac ggcggctata aagcaattaa ttacggttac accgacgatc
    10441 gtgtttacag caaattgacc agcgaaaacc cgattgatct ggtgcgttat cagttagcta
    10501 actgctatat gggtcgggct gggttgataa actccggcgg tgctgcgggc ggtgaaactg
    10561 acctcagcga tgcagtgcgt actgcggtta tcaacaaacg cgctggcgga atggggctga
    10621 ttcttggacg taaggcgttc aagaaatcga tggctgacgg cgtgaaactg attaatgccg
    10681 tgcaggacgt ttatctcgat agcaaaatta ctattgcctg atacgcctca tacgaacagc
    10741 accaccccgt aggacggata aggcgtttac actgcatccg gcaacctatg cctgatgcga
    10801 cgcggaagcg tcttatcatg cctacatagc actgccacgt atgtttacac cgcatccggc
    10861 ataaaaacac gcgcactttg ctacggcttc cctatcggga ggccgttttt ttgcctttca
    10921 ctcctcgaac aattttcata ttgtcgtttt tgtgatcgtt atctcgatat ttaaaaacaa
    10981 ataatttcat tatattttga aatcgaaaac aaacgacagg atatgaaaat gtacgtggta
    11041 tcgacaaagc agatgctgaa caacgcacag cgcggcggtt atgcggttcc ggcattcaat
    11101 attcacaatc tcgaaacgat gcaagtggtg gtagaaaccg ctgccaacct gcatgcgccg
    11161 gtcatcatcg ccggaacgcc tggcacattt actcatgctg gtacagaaaa tctgttggcg
    11221 ctggtcagcg cgatggcgaa gcaatatcac catccactgg caattcatct cgaccatcac
    11281 acgaaatttg acgatatcgc tcagaaggtt cgttctggcg tgcgctcagt catgattgac
    11341 gcctcgcatt tgccttttgc gcaaaatatt tcacgggtca aagaggtggt ggatttttgc
    11401 catcgctttg atgtcagcgt cgaagcggag ctggggcaac ttggcggcca ggaagatgat
    11461 gtgcaagtca atgaagccga tgcgttgtac accaaccccg cacaggcgcg tgaatttgcc
    11521 gaggcaaccg ggattgattc cctggcggta gccatcggca cggctcatgg tatgtatgcc
    11581 agcgcaccgg cgcttgattt ttctcgactg gagaacattc gccagtgggt gaacttaccg
    11641 ctggtgctgc atggcgcgtc agggttatcg actaaggata ttcagcaaac catcaaactg
    11701 gggatatgca aaatcaacgt tgcaacggag ctgaaaaatg ccttctcgca ggcgttaaaa
    11761 aattacctga ccgagcaccc cgaagcgacc gatccccggg attatttgca gtcggctaaa
    11821 tccgcaatgc gcgatgtggt gagcaaagtg attgccgatt gtggctgcga gggcagggca
    11881 taacgcacct gccatttaat aaggaaaaaa catgaaaacg ttaattgccc ggcataaagc
    11941 tggtgaacat atcggcatat gttcagtctg ttctgcccat ccgttggtta tcgaagcggc
    12001 gctggcattt gatcgcaaca gcacgcgcaa agtgctgatt gaagcaacgt caaaccaggt
    12061 caatcaattt ggcggttata ccggaatgac accggcagac tttcgcgaat ttgtttttac
    12121 gattgccgat aaagttgggt ttgcacgcga acgcattatt ctcggcggcg atcatctggg
    12181 gccaaactgc tggcagcaag aaaatgcgga tgcggcgatg gaaaaatccg tcgagctggt
    12241 aaaggaatat gttcgtgccg gtttcagtaa aattcatctt gatgcgtcaa tgtcctgcgc
    12301 gggggatccc ataccgttag caccagaaac ggttgcggaa cgagctgctg tgctttgctt
    12361 tgctgcggaa agtgtggcga cagattgcca gcgtgagcaa ctgagctatg tcattggcac
    12421 cgaagttccg gttccgggcg gtgaggccag cgccattcag tcagtacaca tcacccatgt
    12481 tgaagatgcc gccaatactt tacgtacgca tcaaaaggcc tttattgccc gtgggctgac
    12541 agaggcgtta acacgcgtga ttgccatcgt ggtgcagccg ggcgtggaat ttgatcacag
    12601 caatattatc cattatcagc cgcaggaagc gcagccgctg gcgcaatgga tagaaagcac
    12661 ccgaatggtt tatgaagcac attctaccga ttaccagacc cggacggctt attgggaatt
    12721 agtccgcgat cactttgcaa tattgaaagt cggtcccgca ttaacctttg ctttacgtga
    12781 ggcgatattt gcgctggcgc aaattgagca ggaacttatc gcccccgaaa atcgcagcgg
    12841 ttgcctggcg gtaattgaag aagtgatgct cgacgaaccg caatactgga aaaaatatta
    12901 tcgtacgggt tttaacgatt cattactgga tattcgttac agcctgtcgg atcgtattcg
    12961 ttattactgg ccgcatagtc ggattaaaaa tagcgtcgaa acgatgatgg tgaatcttga
    13021 aggcgtggac atcccactgg gcatgattag tcagtatctt cccaaacaat ttgaacgcat
    13081 tcagtccggg gaattatcag caataccgca tcagctgatt atggataaaa tttatgatgt
    13141 tttgcgcgcc tatcgctacg gctgtgcgga ataaggacgg tatatgacta acctgtttgt
    13201 tcgtagcgga atttcttttg tcgatcgtag cgaagtttta acccatatcg gtaatgagat
    13261 gctcgccaaa ggtgtggttc atgatacctg gccacaggca ttaattgcca gagaagcaga
    13321 attccctacc gggataatgc ttgagcagca cgccattgca ataccgcatt gtgaggcgat
    13381 tcatgctaag tcgtcagcca tttatctgtt aaggccaaca aataaagttc attttcagca
    13441 agcggatgat gataacgacg tggaggtatc gttggttatt gcgttgattg ttgaaaaccc
    13501 gcagcagcaa ttgaaacttt tacgctgttt atttggcaag ttacaacagc ccgatatcgt
    13561 cgagacacta atcactcttc ctgaaactaa gttaaaggaa tacttcacaa aatatgtttt
    13621 agattcagac gaataaatcc ctctgtaaca ataaaaagga ctatttatga aacgcaagat
    13681 tattgtcgct tgcggaggcg cggttgcgac ctctacgatg gcggcggaag aaattaaaga
    13741 gttgtgtcag agtcataata ttcctgttga attaatccag tgtcgggtta atgaaataga
    13801 aacctatatg gatggtgtgc atttgatatg taccactgcc agggtggatc gcagttttgg
    13861 cgatattccg ttagttcacg gcatgccttt tatttctggt gtcggtatcg aagcattaca
    13921 aaataaaatt ctgactatct tacaggggtg acctatgttt tcagaagtca tgcgttatat
    13981 tctcgacctc ggccctacgg tgatgcttcc gattgtcatc attatttttt ctaaaatatt
    14041 aggcatgaag gcaggcgatt gctttaaagc gggtctgcat atcgggattg gctttgttgg
    14101 cattggcctt gtgattggct taatgctgga ttccattggt ccggcggcga aagcgatggc
    14161 ggaaaatttc gacctgaatc tgcatgtggt cgatgtcggc tggccgggct cttcaccaat
    14221 gacctgggcg tcgcaaattg cgctggtggc gattccgatt gcgattctgg ttaacgtggc
    14281 gatgttactg acccgtatga cgcgggtggt aaatgttgat atctggaata tctggcatat
    14341 gaccttcacc ggcgcgttgc tgcatctggc aaccggttca tggatgatag ggatggcggg
    14401 tgtggtaatt cacgcggcgt ttgtttataa gctcggcgac tggtttgccc gcgatacccg
    14461 aaatttcttt gagctggaag gcattgctat tccgcacggt acgtcggcgt atatggggcc
    14521 gattgcggtg ctggtcgata ctatcatcga gaaaatccca ggcgttaacc gaattaaatt
    14581 tagcgccgac gatattcagc gtaaatttgg tccgtttggc gagcctgtca ccgtgggttt
    14641 tgtgatgggg ctgattatcg gcatcctcgc gggttacgat gtcaaaggtg tattgcagct
    14701 ggcggtaaaa acggcggcgg tgatgctgct gatgccacgg gtgattaaac ccatcatgga
    14761 tggtttaacg cccatcgcta agcaggcccg tagtcgttta caggcgaagt tcggcggtca
    14821 ggagttcctg attggcctgg atcccgcatt actgctgggg catacggcgg tggtatcggc
    14881 aagcctgatt tttattccgc tcaccatttt aattgctgtt tgtgttccgg gtaatcaggt
    14941 actgccgttt ggcgatcttg ccactatcgg cttcttcgtg gcgatggcgg tcgcggtgca
    15001 tcgtggaaat ctgttccgca ccttaatctc gggtgtcatc attatgagca tcaccctgtg
    15061 gatcgcgacg caaactattg gtttgcacac ccaactggcg gctaatgctg gggcgttaaa
    15121 agccgggggt atggtggctt caatggatca gggcggttct cccattacct ggttactgat
    15181 tcaggttttc tccccgcaaa atattcccgg tttcattatt atcggcgcaa tttatctgac
    15241 cggtattttc atgacctggc gtagagcgcg tggttttact aaacaagaga aagccgttct
    15301 cgcagaataa tttttacctg agggggaggt tatccccctt aacaatcagg agtttttatg
    15361 aaatcagtag tgaatgatac tgatggtgtc gtgcgcgttg caaaaagcgt cattcctgaa
    15421 attaaacatc aggatgaggt gcgggtaaaa attgccagct cgggcttatg tggttccgat
    15481 ttacccagga tatttaaaaa tggtgcacat tattatccaa taacgttagg ccatgaattt
    15541 agcggctata ttgatgcggt aggatccggt gttgatgatt tacaccctgg cgatgcggtt
    15601 gcctgtgtgc cgttattacc ctgttttact tgtccagagt gtttgaaagg gttttattcc
    15661 cagtgcgcaa aatatgattt tattggctcg cggcgtgatg gtggatttgc cgaatatatt
    15721 gtcgttaacc gaaaaaatgt ctttgctcta cccacggata tgcctattga ggatggggct
    15781 tttattgagc cgattaccgt tggtctgcat gcttttcatt tagcgcaagg ttgtgagaat
    15841 aaaaacgtta ttattattgg tgccggaacc attggcctgc tggctattca gtgcgctgtc
    15901 gcgctgggag caaagagtgt gacggctatc gacattagct cagaaaaact ggcactggca
    15961 aaatctttcg gtgcgatgca aacatttaac agtagcgaaa tgagcgcgcc gcaaatgcag
    16021 agcgttttac gcgaactgcg ttttaatcag cttatcctcg agacggctgg cgtaccgcaa
    16081 actgtcgaac tggcggtaga gattgccggt cctcatgccc aactggcgct ggtgggcacg
    16141 ttgcatcagg atctgcattt aacatcggca acgtttggca aaatattgcg taaagagctg
    16201 acggttatcg gcagttggat gaactattcc agcccttggc cggggcagga gtgggaaacg
    16261 gcgagccggt tgctgacaga acgtaagtta agcctggagc cattaatcgc tcaccgtgga
    16321 agctttgaaa gcttcaccca ggcggtgcgt gacatcgctc gtaatgccat gccgggcaaa
    16381 gtgttgctca ttccatgaaa ccgcgggcca gcgtgatgct ggcccggtat tgtgcaaaac
    16441 agatcattca ccaatggtcc cccttcgttt acactagcca caattgaata tgggtaaatg
    16501 acgatgaatt cattcgagcg aaggaataag atcatccaat tagtgaatga acagggaacc
    16561 gtgcttgttc aggatctggc gggagtattt gctgcctcgg aagcgacaat ccgtgccgat
    16621 ttgcgctttc tcgaacaaaa aggcgtggtt acgcgctttc atggcggtgc ggcgaaaata
    16681 atgtccggta atagtgaaac cgagacccag gaagtcgggt ttaaagagcg atttcagctc
    16741 gccagcgcgc ccaaaaacag aatagcgcag gcggcagtca aaatgatcca tgaagggatg
    16801 accgttattc tcgacagcgg aagtaccaca atgcttatcg ccgaaggatt aatgaccgcc
    16861 aaaaatatca cggtgattac caacagtctc ccggcagcgt ttgccctttc cgaaaataaa
    16921 gacattactc tggtcgtctg tggtggcacg gtccgccata aaacgcgctc gatgcatggt
    16981 tccattgccg agcgttcatt gcaggatatt aatgccgatt taatgtttgt cggtgctgat
    17041 ggtattgatg cggttaatgg tattacgacc tttaatgagg gttattccgt tagcggtgcg
    17101 atggtcacag ccgctaataa agtgattgcc gttctcgatt catcgaaatt taaccgtcgc
    17161 ggttttaatc aggtactgcc aatcgaaaaa attgacatta ttattaccga tgatgctgtg
    17221 tctgaggtgg ataaactggc attgcagaag acacgggtga aattaattac ggtgtagtat
    17281 tgttctgtat gccggataag gtatttacac cgcatccggc attacggatt ctccctggtt
    17341 caagagagaa tagtttgatt aacgcaataa tggacaatct ggtggtaatc gacaacgcaa
    17401 cgccgccgga agtatttcaa tatgaaagtt ttgtccgctc aacggttcgc catcaagatt
    17461 aaaagtgatt tcgtgtgggg cttgtatatc aaaccacgac gaagcgcctt cgataatatt
    17521 cgggttatct tcgtcagatt ttaaggttga tacgagagcc ggaagaattt catcgccggt
    17581 aaaaatgcgc agttgcagca agccatcgtt aattaacgcg ttcggacaca attgctgacc
    17641 gccaccggcc tgacgcccgt taccaatacc aatgaccagg gcgtcacctt gccagtgaaa
    17701 gttttcaccg cggatttcac aacggtccgg ttgcagagta tccatgcgca ttaagccatg
    17761 aatgatgtaa gagacgccac ccagcgcggc ttttaatttt tccggcgttt ctgtggtaat
    17821 acgcgtccca aatccgcctg tcgccatatt aataaaacag gtttgtttgt tgacctgcgc
    17881 catatctatc gcaatggcgt taccggcaat tgccagtttc agcgccttat ccagtgcctc
    17941 aggaatccct acactggtgg caaaatcatt ggcggttcct aatggcaaaa ttcccagcgc
    18001 gggtatgtca tccccctcac actgaatcaa cgccgtagaa acttcattaa tggtgccatc
    18061 gccaccaccg gcaatcaccg ttgcgacgcc caacttccgg gcctcctcta catatcgtgc
    18121 ggcatcgcct ttctcccagg tgacccgcac atggatcgtc attccttcct cacgcaacag
    18181 cataattgct tcgcgcaagg gtagattgtc agtacttttg ccattaagaa tcagtaagct
    18241 ggcgggaaat tctgccatga tcgtatgtgc ctttatgatt ggtctgtaga tagtgtagag
    18301 cagaaaacaa aaggtggagt cagaacagga tgaaagtcgg aggataaaaa gtcagttcaa
    18361 gtatggagga ttcagtggtt accaccagga gcttgcaatg gggacagcga atactaacgg
    18421 ctgccaccga taaatttcaa aaaagagcat atgcctaata ttcaaccaaa cagtggcatc
    18481 ttaaatatac tattccaaat atattaaagc ccccatggag ttaccctgaa gggcctcaat
    18541 gtccgtaatt cctacttatg tag
//
LOCUS       DAPHDW010000083        18452 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.083, whole genome shotgun sequence.
ACCESSION   DAPHDW010000083 DAPHDW010000000
VERSION     DAPHDW010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18452
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.083"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            7..1305
                     /gene="dadA"
                     /locus_tag="RH057_002892"
     CDS             7..1305
                     /gene="dadA"
                     /locus_tag="RH057_002892"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HDV3129232.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVEPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKCGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGRTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            1315..2385
                     /gene="dadX"
                     /locus_tag="RH057_002893"
     CDS             1315..2385
                     /gene="dadX"
                     /locus_tag="RH057_002893"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309712.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HDV3129233.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQPDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISSAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGTPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(2771..4507)
                     /locus_tag="RH057_002894"
     CDS             complement(2771..4507)
                     /locus_tag="RH057_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HDV3129234.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(4602..5516)
                     /gene="ldcA"
                     /locus_tag="RH057_002895"
     CDS             complement(4602..5516)
                     /gene="ldcA"
                     /locus_tag="RH057_002895"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HDV3129235.1"
                     /translation="MSLFHLIAPSGYCIKQHAALRGIHRLTDEGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNKTFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINEHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGTQLTISGHPVLKM"
     gene            5616..6227
                     /gene="emtA"
                     /locus_tag="RH057_002896"
     CDS             5616..6227
                     /gene="emtA"
                     /locus_tag="RH057_002896"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HDV3129236.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(6229..6963)
                     /locus_tag="RH057_002897"
     CDS             complement(6229..6963)
                     /locus_tag="RH057_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HDV3129237.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISISERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            7164..7418
                     /locus_tag="RH057_002898"
     CDS             7164..7418
                     /locus_tag="RH057_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV3129238.1"
                     /translation="MGIIAWIIFGLIAGIIAKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIGGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            7596..8036
                     /locus_tag="RH057_002899"
     CDS             7596..8036
                     /locus_tag="RH057_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129239.1"
                     /translation="MIQKINILDSSQSGVKNGADSEAVLAALIEHLELINPSGRLSQN
                     TRSAMLQLREEEWSEFFFWLLNSLECLDYVIINLTPESKKTLMSEHRNNIQVAIDALY
                     RQRRYKSPGDDSETLTRRNDAIFGNHVWQTFAQYFPPGLEKPSV"
     gene            complement(8115..9812)
                     /gene="treA"
                     /locus_tag="RH057_002900"
     CDS             complement(8115..9812)
                     /gene="treA"
                     /locus_tag="RH057_002900"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HDV3129240.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNAKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     SYFTMLGLAESGHWDKVADMVANFAHEIDTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDGTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATRPTVKSATTQPSTKEAQPTP"
     gene            complement(10132..11550)
                     /gene="dhaM"
                     /locus_tag="RH057_002901"
     CDS             complement(10132..11550)
                     /gene="dhaM"
                     /locus_tag="RH057_002901"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HDV3129241.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPKIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTKISDTCPAYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRQLAEDNFGETEEVVPPTLRPVPPVSGKAFYYQPVLCTVQAKSTL
                     TVEEEQERLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLDDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            complement(11561..12193)
                     /gene="dhaL"
                     /locus_tag="RH057_002902"
     CDS             complement(11561..12193)
                     /gene="dhaL"
                     /locus_tag="RH057_002902"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HDV3129242.1"
                     /translation="MSLSRTQIVNWLTRCGDIFSTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGVDGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASR
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            complement(12204..13274)
                     /gene="dhaK"
                     /locus_tag="RH057_002903"
     CDS             complement(12204..13274)
                     /gene="dhaK"
                     /locus_tag="RH057_002903"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415718.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HDV3129243.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRSFSSLDQTVDEMFDTLLENGSYHRTLRFWDYQQGSWQEEQQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            13502..15421
                     /gene="dhaR"
                     /locus_tag="RH057_002904"
     CDS             13502..15421
                     /gene="dhaR"
                     /locus_tag="RH057_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HDV3129244.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKQAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPVDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            complement(15521..18388)
                     /locus_tag="RH057_002905"
     CDS             complement(15521..18388)
                     /locus_tag="RH057_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3129245.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYSKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKITTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSGSDAIINFTGTAAQRNS
                     IFSGGSYGASAQTATAVVNMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFATLNVENLSGNSTFIMRADVVGEGNGVNNKGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPTPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IGSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNIYVKTGAIREFSGDTEYLLNNSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
BASE COUNT         4456 a         4620 c         4793 g         4583 t
ORIGIN      
        1 gtggctatgc gagttgtcat actgggaagt ggtgtggtag gcgttgccag cgcctggtac
       61 ttaaatcagg caggacatga ggtcaccgtc attgatcggg agccgggggc cgccctggaa
      121 accagcgccg cgaatgccgg gcaaatctcc cccggatatg ctgcaccgtg ggcggcacca
      181 ggtgtgcctt taaaagcgat taaatggatg ttccagcgcc atgcgccgct ggcggttcgt
      241 ctcgacggta cgcagttcca gttgaaatgg atgtggcaaa tgttacgtaa ctgcgacacc
      301 agccactaca tggaaaacaa agggcggatg gtgcgtctgg cggaatacag ccgtgattgc
      361 ctgaaagcat tgcgcgccga aaccaatatt cagtatgaag ggcgtcaggg tgggacgctg
      421 caactgttcc gtaccgaaca acagtatgaa aatgcgaccc gcgatatcgc cgtgctggaa
      481 gatgccggcg taccgtatca gctgctggaa tccagccgcc tggcggaagt ggagcccgcg
      541 ctggcagaag tagcgcacaa actgacgggt ggtctgcaac tgcccaatga tgaaaccgga
      601 gactgccagc tgtttaccca gaatctggcg cggatggcag agcaggcggg ggttaaattc
      661 cgctttaata cgcccgttga ccaactgctt tgcgacggcg agcaaatcta cggcgtgaag
      721 tgtggcgatg aagtgattaa ggccgatgcg tatgtgatgg cgtttggttc ttactcgacg
      781 gcgatgctca aaggcattgt tgatattccg gtgtatccgc tgaaaggcta ctcgctgact
      841 attccaattg cgcaggaaga tggtgcgcca gtatccacca ttcttgatga aacctacaaa
      901 atcgccatta cccgtttcga taaccgcatt cgtgttggcg gaatggcgga aattgttggt
      961 tttaacaccg agctgttgca accgcgtcgt gaaacgctgg aaatggtggt tcgcgatctc
     1021 tatccacgcg gcggtcatgt cgagcaggcg actttctgga cgggtttgcg cccgatgacg
     1081 ccagacggta cgccggttgt cgggcgtaca cgctttaaaa atctgtggct gaataccggt
     1141 cacggcacgc tcggctggac gatggcttgc ggttccggtc agttgttaag cgatctgctc
     1201 tctggtcgca cgccagcgat cccatatgag gatctaagcg tagcgcgcta cagccgtgga
     1261 tttacgccat cacgtccggg ccatttacat ggcgcacaca gctaaggaaa cgagatgacc
     1321 cgtccgatac aggccagcct cgatctgcag gcattaaaac agaatctgtc cattgtccgc
     1381 caggccgcgc cgcacgcgcg cgtctggtcg gtggtaaaag cgaacgctta cgggcatggt
     1441 attgagcgta tctggagcgc gctcggggcc accgatggct ttgcattact taacctggaa
     1501 gaggcaataa cgttacgtga gcgcggctgg aaggggccga tcctgatgct ggaaggattt
     1561 ttccatgctc aggatctgga gatttatgac cagcaccgcc tgaccacctg cgtacacagc
     1621 aactggcagc tcaaagcact gcaaaatgcg cggctaaaag caccgttgga tatttatctt
     1681 aaagtgaaca gtgggatgaa tcggttgggc ttccagcccg atcgcgtgct taccgtctgg
     1741 cagcagttgc gggcaatggc gaatgttggc gagatgaccc tgatgtcgca ttttgccgaa
     1801 gcggaacatc ctgatggaat ttccagcgcg atggcgcgta ttgagcaggc ggcggaaggg
     1861 ctggagtgtc ggcgttcgtt gtccaattcg gcggcgactc tgtggcaccc ggaagcgcat
     1921 tttgactggg ttcggcctgg cattattttg tatggcgctt cgccgtccgg tcagtggcgt
     1981 gatatcgcca ataccggatt acgtccggtg atgacgctaa gcagtgagat tattggtgtc
     2041 cagacgctaa aagcgggtga gcgtgtgggc tacggcggtc gctatactgc gcgcgatgaa
     2101 cagcgaatcg gcattgtcgc cgcagggtac gccgacggtt atccgcgcca cgcgcctacc
     2161 ggtacccctg ttttagtgga cggcgtgcgc accatgacgg tggggaccgt ctcgatggat
     2221 atgctagcgg tcgatttaac gccttgcccg caggcgggga ttggtacgcc ggttgagctg
     2281 tggggcaagg agatcaaaat tgatgatgtc gccgccgctg ccggaacggt gggctatgag
     2341 ttgatgtgcg cgctggcgtt acgcgtcccg gttgtgacag tgtaacttgt tgtaagccgg
     2401 atcggagaca gcgtcttctg gttgcgaaaa aatcatccat ccggctggtc agcaactgta
     2461 gttgttaatg tgacagagcc attgcccatg atagtgtcca ttaaaagaat ggacactatt
     2521 tccccggaac ctgaactcac cgcacaggcg ttctacataa aacgcttacg cttcattgtt
     2581 gactcgaact cgacttcaga taaatcacgc tttacccttg atggagcctg tacatagatt
     2641 tgtgtaattg cctgattttg atatgttcaa ttcaacatca aatgaaggtt aaattatgga
     2701 cgacaaacaa ttgcaggctc aggctgcgtt cagcaaagca tcgcaaccgg cgatagatgc
     2761 ttcattaaat ttaagattca gcttcctctt cagccacccg tacgccaatc ttcaacactt
     2821 cattatcttc tttctcggcc accgtccaga tcatcccggc aaactctact tggtcaccga
     2881 caaccggtgc cgcgcctaac aactgctgga caatttcacc cagcgtttgc tgcttatcac
     2941 gatattctcg cccgtcttcc aggccatata tcagcgcaac atcagcatat ttggcgctgg
     3001 cttcgagaat gaagtcacca aagaagcgtt gatccagcgc gaccggcggc gactggctga
     3061 acagtttacc gagcgccgga agatcgcgtt cccgaccaat tacacacaac acatcgcctt
     3121 cgcgcagtcg ggtgctgccg gtaggatgaa gcaactggtt atcacgaaac agtgccgcaa
     3181 tacgcgtctc ttttggcata tgcaaatcgc gcagtgccgc gcccacgcac catttatcgg
     3241 cactcaattg ataaacaaac tgctcccacg gattttccgg atgaatatcc aggccaacgc
     3301 gtgacaccgg acgtcccact ggcggaacca ccactttggc ttttttagcc gcccacgaga
     3361 gtgatgttcc ctgcaagagc agtgaaacca gaaccacaaa gaaggcgaca ttaaagaaca
     3421 gacgtgcatt ctccagcccc gccatcatcg ggaacacggc caggatgatt ggcaccgcgc
     3481 cgcgtaatcc tacccagctg ataaacacgc gctcacgcag attgaaaccg cggaagggga
     3541 gcaatccagc aaataccgaa aggggacggg cgaagaatat catccatgcc gacaaaatga
     3601 gcgccggaat ggcaataggc agcagatcgc ttgggttaac caatagcccc agcaccagga
     3661 acatggcgat ttgcgccagc caggcgaggc cgtcgaaatt ttgcaggatg ccgtagcggt
     3721 tgcgaatcgg ccgattaccc agcagaaaac cgcacagata aacagccaga ataccgctgc
     3781 cttccagcgc cgtggttaaa gcaaaaatta aaataccgcc gcttaatgcc agcaatggat
     3841 ataatccggc aggcagggcg atgcgattaa tcatttgcag cagtaaataa ccaccgccaa
     3901 gtccgatgac aatcccgagg ccaaattgct gcagaatatc gaccacgaac atccaactga
     3961 cactgctttc atgttgctgg atcatcgcaa tcagggtaat cgtcagaaag accgccattg
     4021 gatcattact gccggattca atttccagcg tcgagccaac acgttcgtta agccccttac
     4081 cacccagcag agaaaagacc gctgcagcat cggtagagcc gacgatagcg ccgattaata
     4141 agccttcaat caaatcaaga ttaaacagcc acgccgccat catgccggtt aaaccagagg
     4201 tgataagcac gcccagcgtc gccagcgaca gtgccggtcc taacgccaca cgaaaggaac
     4261 tggcctgagt gcgcatcccg ccgtcgagca aaataatcgc cagtgccagg ttacttacca
     4321 tgtaggcgaa ggggtaatta tcaaacggga taccgccgac gccatcgact cctgccagca
     4381 tgccgatcgc cagaaaaata accagaatag gaatgccaag acgggaagaa aatgaactaa
     4441 gtaatatact gctggtgaca aggatggatc ctaagatgaa aaggctaatt attgttgtgg
     4501 catccaacgg tcggttactc ctgattacgc tgtctcttat ataaacccta ccatattagc
     4561 ggcagagaca gcgttttact tagccctgaa gcgtgatttt tttacatttt aagaacagga
     4621 tgaccgctaa tcgtcaactg agtgccttcc cgggtgttat tcagaatggc atgtgcgccc
     4681 aacggcagcg taaccgtgcg ttgttcatga ccaaaatcga ggccggtaat gagtggaatc
     4741 gacaggcggg aacgcaaaaa cgcgtacact gactccaggt tgtaacctgc gtcataatca
     4801 ttgggcgtgc tgccgctaaa gctaccgaga ataatcgcct tctgacgtgg caaaattccc
     4861 gcatgataga gctgcaacag catacgttcg acgcggaaag ggtgctcgtt aatatcttcc
     4921 agtaccagga taccgttctc aatttttggc atccacggtg taccaatcag tgaaatcagc
     4981 atcgcaagat tgcctcccca caacgtgcct tcggtctgac atgtcgggcc ttccccttgc
     5041 cattcaatgg tgaaggtttt attgcgtaac gccagccaga aatggtgctc ggtaaaggcg
     5101 ttcagttcat ccgcaccaaa gtttgctacc agcatggggc cgctaaaggt gatgacattg
     5161 ccttgcgcca gaagaccgca ctgaatggcg gtaaaatcgc tatgtccgca aatgagtaac
     5221 gggtcatgtt gttggcgagc caccagtgcc tgccagtcaa tatctgccag taaacgactg
     5281 gcaccgtaac cgccgcgtac agccagcacg atggtgttgg gggtagtcag tctggcaagg
     5341 gaattgagat cttccaggcg ctccgtttcc gtacccgcaa aacgctcaca acgacgggca
     5401 ataacctcga cgttattgac ctgatgcccc tcgtcggtaa ggcgatggat accacgcagc
     5461 gcggcgtgct gtttaatgca gtaacccgat ggggcaatta agtgaaacag agacatggca
     5521 attccttgct gacaacagaa acgaaatgta tatcatgccg cttaggtgtg ccgttgtcac
     5581 ctcaacggcg attccaggct ataaggatag aagaagtgaa attgagatgg tttgcctttt
     5641 tgattgtgtt attagcgggt tgttcatcaa agcatgacta tacgaacccg ccgtggaacg
     5701 cgaaagttcc ggtgcaacgt gcgatgcagt ggatgccaat aagccagaaa gccggtgcag
     5761 cctggggcgt cgatccacaa ttgatcacgg cgattatcgc tatcgaatcg ggtggtaatc
     5821 cgaacgcggt gagtaaatcg aatgccattg gtttgatgca gttaaaagct tcaacctccg
     5881 gacgtgatgt ttatcgccgt atgggctgga gtggtgagcc gacgaccagc gagctgaaaa
     5941 atccagagcg taatatttca atgggggcgg cttacctgaa tattctggaa accggcccgc
     6001 tggcaggcat tgaagatccg aaggtactgc aatatgcgct ggtggtgtca tacgctaacg
     6061 gggcaggtgc gctgctacgg actttctcgt cagatcggaa aaaggcgatc agcaaaatca
     6121 acgatttgga tgctgacgag ttcctcgatc acgtagcgcg aaatcatcct gcgccgcagg
     6181 ctccgcgcta tatctacaaa cttgagcagg cactggacgc gatgtaaatc agtcgcgcac
     6241 tttgtccgct ttttcccggg cttctcgctc gagagagaaa ataatccgct gtaattgccg
     6301 ctccaccgtc gggctgacgt taagaaaacg gaagctcaga cggggagtgg tgatggtttc
     6361 attcttgcca tcaatcactt tgcgctcgct gatggagatt aactgggcgt caaagtgaaa
     6421 aacaccccat tgccccatgt tgacttcaat ctgagcgaag cgcatgcctt cttgtaattc
     6481 ggcaggcttt gctgtttcca gtaatgcgcc catgccgcct aacgacaaat catacaggcg
     6541 gaaacgtaac gtactgttat ccgccagttt ggtctggcaa aaataaggcg gatggagtgg
     6601 ggcggagatg cggaaatatc ggcgtcgttg tacaaaccat aaggtgggag gcggtacggt
     6661 gataaatgcc ggaagctgca agtattcact ctgctgtagt tgttcaacag taaactcgac
     6721 tttcgcaccc tgagtttcgg cggtaatggt aatgtgctgt gcctttagca cggcgatgtt
     6781 gtcttcggct tgactgccga aatccagcac cagtttatcc ggggttattg ccagtatttt
     6841 gctgatcagc tgcccgccat tccaactgag acgcaaagga attgcggctt tgtgcaaatc
     6901 gcgtaacacg cccaggacgg ctaacggatt ttgtttcagg aactgctcat ggtaatgact
     6961 cacgcggaaa aactcctgac tgataaactg tctttggttt atcggtcaca gttaacaaaa
     7021 cttaatacaa atgcgtgaat attttttaca tgttgttctt aaatcagccg acatacgccc
     7081 agcatcgatc ccgcccttgc ctatacttag agcgttgacg taagcatttc ttgcgtccga
     7141 ttcatcgaaa cgagggcctg aacatgggaa ttattgcctg gattattttt ggcctgatag
     7201 ccggcattat cgccaagcta atcatgccgg ggcgtgatgg tggtggattt ttcctgacct
     7261 gtattctcgg gatagtcggt gcggtggtcg gcggctggct ggcgaccatg tttggcattg
     7321 gcggctccat cagtggtttt aatctgcaca gcttcctggt ggcggtggtg ggagctattc
     7381 tcgttctggg cgtattccgc ctcctgcgaa gagaataaga ttttcataag gcggatagcg
     7441 atacagatgc cgctatccgc tttcacatca gaacgtatac tcgacacctg ctttacgggt
     7501 ggaacaaatc aatatagcac ttagatgccc gataaagtcg gttcccggta acaacaacag
     7561 aatccttttt taattattgt ttcgttgttt ttggtgtgat tcagaaaata aatattttag
     7621 acagtagcca gtcaggcgtt aaaaatgggg cggatagtga ggcggttttg gctgcgctta
     7681 ttgaacattt agaattgatc aatccttctg gcagattaag ccaaaatacc cgttcggcaa
     7741 tgcttcagtt acgcgaagag gaatggtctg aattcttttt ttggttgcta aattctctgg
     7801 agtgtctgga ttatgtgatt attaatttaa ctccagaaag caaaaaaacg ttgatgagtg
     7861 aacaccgtaa caatattcag gtagcaatcg atgctttata taggcaacgt cggtataaat
     7921 caccggggga tgatagtgaa acacttaccc gtagaaatga cgctatcttc ggcaaccacg
     7981 tatggcaaac atttgcgcaa tacttcccgc caggacttga aaaaccgtct gtgtagaaaa
     8041 caccatagct atggtgttat ctgccgacaa cgccatccgg agaagccgaa tgacgttgtc
     8101 ggagtaagcg ctggttaagg tgtgggttgt gcctctttgg ttgagggttg cgtcgttgct
     8161 gacttaacgg tcggacgcgt cgccggaaca ttgtcacacg gttgctcttt cgggcagatc
     8221 aaatccagca ttttcagcgt cacgccattg gtccagccaa agccatcctg taatggatat
     8281 tcgccaccgc cgccccccgt tccggtggtg ctgacatcat atttttccac cagctttttc
     8341 tcccggtcat aggtgtgctg aacattggtc aggaagtgcc agctaatgtc catcgccacc
     8401 tctttttgcc cgtagttttg taatccttct gtcgcgaccc actgtaacgg tgcccagcca
     8461 tttggcgcat cccattgttg cccacttttc accgacgtgg tgttcaggcc gccgggttgc
     8521 agcagatgtg ttttcgtcgc cgtcgccatt ttgttggcgc gatctttcgc tgccgcattg
     8581 acgtacagcg ggaacagggc ggccgcggtt aactgattgc gcactttatg acttttcagg
     8641 tcgtaatcgg cataccagcc ttgttgatcg ttccacaggt atttttcgat ccctttttga
     8701 cgggcatttg ccagcgtttc gtactggttt gccatcgcgt tatctccggc agctttgctg
     8761 gcgcgggcga ggattttttc cattttaaac atcaggctgt tcagatcgac cggtacgatg
     8821 ctggtggtgc gtaaggtatt taactgctgc gggttgtcca tccagcgcga gctgaaatcc
     8881 cagccagacg cagcggcaga gcgcaggtcg cggtaaattt cagtggcagg tcgattcgga
     8941 ttgcttttgg cggtggcaat atcttccacc catgactctg gtcgtggcgt atcgcgatcg
     9001 tcccagtagc ggttgagaag ggtaccatcc tgaagtttga caacgcgttt ttcctgttgt
     9061 ccggcttgca ggttttcaac accgtccatc cagtaagcat attctttttg catttgcggc
     9121 aggtattgct tcaacgcggc atcgccttca tgctgcgcca gtaactctac catcagggca
     9181 aagaagggcg gttgcgagcg gcttaaatag taactgcggt tgccgttggg aatatgaccg
     9241 taagtgtcta tttcatgagc aaaattggcc accatatccg cgactttatc ccagtgaccg
     9301 ctttcggcaa gtcctaacat ggtgaagtaa ctgtcccagt aatatacctc gcgaaaacgt
     9361 ccgcccggca cgacataagg ttccggcagc ggtaacagag aatcccattt ttctgtgttt
     9421 tcggtagaac gcgttaatac cggccaaagt ccgtcaatat gttcgcgcag tgactgcccc
     9481 tctggcggaa catatttctc gccttctttc ggcagggtga aattgacgtt aacgaaatgg
     9541 cgcagatcaa atccgctctg gttttgctgc atccgataat cagcaaggat catcagcgga
     9601 tcgctgttcg gcacggcatc ggcaaaggtt ttttggtccg gaaaaagttt ggcgttttgc
     9661 acatcattaa acagcggccc taataaaata tcaggcggct gtggtgttac cggtgtttct
     9721 tctgcctgca ccgatagcgc agcgaaacac aaaaagatac aggctggaat taacgccatt
     9781 ttttgcgggc gagaaggtgc gggggatttc atcaatcatt ctcctttggc gaaaccgaat
     9841 aaaacggtat tcaagtatca gaaaacctta gttcaggatc gcactagctg catgatcgaa
     9901 atctcattct gcgaactgtc aggcatttta gaaaagggaa ttaaagggat aaataacggc
     9961 gaaatagagt gatcaaataa cgtctgttta ttagccagcc agcggcgttg ggttcgtgat
    10021 tagcgaagaa aaatcatcat aaattggtga aaaatataac aggtagttaa aaaccattag
    10081 tgctgagtaa attgccggat gacatcagaa cgatgccatc cgaacagtgg cttaaccctg
    10141 acggttgaaa cgttgcgttt taacgtccag cgttagcgtt tcttctggtt gtatcgcata
    10201 cagtttctca ccctgctggc aaatccagcc aatccccagc tcacgggcga ttagggcgct
    10261 gtgggaaacc ggacttccgg cgctaaggca gatacctttt acaaccgccg gatccagttg
    10321 cagtactgtg gaaggataaa tgttctccgc cagtagaata gttggcgagt taaactgcgg
    10381 gagttcttct ttcgtttggg tcaggtggac cagggtgcga tgcagaagat cgtccacatc
    10441 aatatagcga gcttgtagat attcatcatc cagttgctgg tattgctggc taagttcttt
    10501 aagaacttgc tgccaggcat attctgccgt gcaatgttcg tgctgaagga gttcgcttgc
    10561 cgccgccagc agttccggat catctaacag tgtatggtga ccagaaaaga ttgcggcaat
    10621 atcgtcaagc ccgctggctt ctgctttcgc tgttaacgtc atcagatcta ataacgtgaa
    10681 gtcaatggcc tggcgtaatc gttcttgttc ctcttccacg gtcagggttg attttgcctg
    10741 taccgtacat aaaaccggtt gataataaaa ggctttaccc gaaacaggcg gaacgggacg
    10801 cagagtaggt ggaacaactt cctccgtttc accaaagtta tcttcagcca gctgacggaa
    10861 agcgatcagt gcctcttcag cttctggccc tttcgcaatc aggcgcagcg tatcgttata
    10921 gcgaacttgt agtaacgcaa tctggttaat actctctggt gtgacgcatt tgccgttttt
    10981 ttccagcaac atatcggcat taaatgtcga taaggtataa accagccggg aggccggacg
    11041 tacatgcagg ccgttacggt tttttatgac caccgccaga gaacgggctt cttcatcgta
    11101 cgcaggacat gtgtcagaga ttttagtgtc ggaggacggt aaacccagtt gttcacgttt
    11161 ggcttccagc gcatgcatag cgtcaaagat aactttgtcg atatccgctc ccgaggccgc
    11221 gctgaccgtt gctgccagtg taccttcgac caacggcgca gcacacaaac gtacttttgc
    11281 ggcgatcttg ggagccagca attccagcgc agtttcagca ctcaataatg cgctacccat
    11341 atccatcatg accagcacat ggtcggcatc agcaacagat tcgatggcct ccatcacttt
    11401 gacggcatcg gtaccaatag gattttgtgg atcgtcaatt cccgcggcaa tggcgatttt
    11461 acaactatca ctcattaaca tctgacgggc taattcaccg acaccttctc ccagtcggct
    11521 gctatgtgaa actatgacca ggtttaccat caccaattcc ttactctttt gcggctaacg
    11581 ccaacatttg catcataaac atcaccgagg tcgcgccggg atcctggtga ccaatactgc
    11641 gttcaccgag ataactggcg cggcctttgc gggcttgcat cgtaatcgta ctttgtgcag
    11701 cggattcggc gattctactg gcagcttcga gcgccaccgg aacagagaga ttttgctcgc
    11761 tggactgacg taacgattcc accaccggca cccacacatc acacatggtt ttatcgccag
    11821 gttcggcttt cccgcgactg attacgccgt ccacgccatc gcggaacatc tgataaagct
    11881 cttccagtgt caggctttgc cgtgcctggg tcgcctgtgc ggcgcggata aagaaggtac
    11941 cgaacagcgg accactggca ccaccgacgc tggaaagcag cgtcataccg gtattcttga
    12001 gaatgaaacc gatatcttta tctgcgatag cagggagttt ttccaccact ttgctaaagc
    12061 ctcggttcat atttagcccg tggtcagcgt cgccaatttc gcgatccagt ccggtaagat
    12121 actcgctctc ggtgctgaaa atatcgccac aacgagtgag ccagttaaca atttgagttc
    12181 tgctcagtga cattgctttc tccttattta ccccagttaa gggccggggt gtggaccggg
    12241 gcgtcccaga gtgccagcgt ttcgtcatca actttcagta aggtgattga gaaaccggtc
    12301 atatccagtg aggtgcagta cgcgccaatt aaattacgtt cgatagtcaa tcccgcttgc
    12361 tggcaacgtg tggtcaggcg gttatagacg ccgtacagct cagaaagcgg agttgcgcca
    12421 agattgttaa ccagcgcaat cacccgatcg ccagactgga gcggttgttt ggtttgttgt
    12481 tcttcctgcc aactgccttg ttgataatcc cagaaacgca aagtgcgatg gtatgagcca
    12541 ttttccagca gggtgtcgaa catttcatcg acggtttgat caagggaaga gaaggagcgg
    12601 cggtcaatac ccggctcacc atgaatgccg acgccaaact ccatctcatt atccgccagg
    12661 gtaaaagaag gtttgcccgc ggcaggaacg gtacaggcac cgagagcgat acctattgag
    12721 tggccttgat tattcaactt acgccccagt tccgcacagg cgtccagtga gtcgccacgc
    12781 tccgccgctg cgcctacgag tttttcaatt aatacggtgt tggcaacgcc gcgtcgcccg
    12841 gcggtataaa gactgtcttt taccgcaacg tcgtcatcaa tgaccacagt ggtcactttt
    12901 acgccgctat cgtgcagtaa ctcggtcgct gtttcaaagt taagaatatc gccggtgtaa
    12961 tttttgataa tcaacagtac accttcgccg ccatcaattt gcatggagca ttcaaagatt
    13021 ttatcgggcg tcggtgaggt gaaaatttcg cccggacagg cccccgaaag catcccctga
    13081 ccgatataac cacagtgcat cggctcgtgt ccgctgccgc cacccgacag cagggcgact
    13141 tttcctgcaa caggggcatc agctcgggtg acatacaccg gatcctgatg cagtgtcagc
    13201 gatggatgcg ctttcgccag tcctgccagt tgttcgtcca gtacgtcttg cacatcattg
    13261 atcaattttt tcattatttt gctccagcaa ttacggtagg gcatggatga tgttcaacga
    13321 cacggcgacc actgactgcc gatgaatcca ttgtgcatca ggcaagggaa aagaaaatta
    13381 gcccaaatta tgtttcatag tgaaacatat gctttaatga atgttccata ttgaaacttt
    13441 tacgtgtatt aatacttaaa attgcgagcc ggaacacctt ttgtcataag ggatgcggga
    13501 tatgagtggc gcttttaaca acgatggtcg gggcatatct cccttaattg caacctcctg
    13561 ggagcgatgc aataagctga tgaaacggga gacatggaac gtaccacatc aggcccaggg
    13621 cgtgacattt gcttctattt atcggcgtaa gaaagcgatg ctgacgctcg ggcaggctgc
    13681 gctggaagat gcctgggaat atatggcacc gcgagagtgt gcgctgttta tcctcgatga
    13741 aaccgcctgc attctcagcc gtaatggcga tccgcaaacc ttgcagcagc taagtgcact
    13801 gggattcaat gacggcacgt attgcgccga gggaattatt ggtacttgtg cgctatcgtt
    13861 agcggctatc tctggtcagg ccgtgaaaac gatggccgat caacatttca aacaggcact
    13921 ctggaactgg gccttttgtg caacgccgtt gtttgacagc aagggccgat tgacgggaac
    13981 aatagcgctg gcgtgtccgg ttgagcaaac taccgcagct gatttgccgt tgacgttggc
    14041 aatcgcccgc gaggtcggaa atttactgct gacggacagt ttgctcgctg aaactaaccg
    14101 tcatttaaat caacttaatg ccctgttaga aagtatggat gatggcgtga ttagctggga
    14161 cgagcagggt aatttgcaat ttattaatgc ccaggcggcg cgggtcttgc gccttgacgc
    14221 gacggcaagt cagggaaggg caatcactga actcttaacg ttacccgccg tattgcaaca
    14281 agcaataaaa caggcacatc cgctcaaaca cgtagaagca acctttgaaa gtcagcatca
    14341 gtttattgat gcggtgataa cccttaaacc gataatagaa acgcagggaa ccagctttat
    14401 tttgttgctc catcctgtgg aacagatgcg gcagttgatg accagtcaat taggaaaagt
    14461 cagccatacc ttcgctcata tgccacagga cgatccgcaa acccgccgct tgattcattt
    14521 tggtcgccag gcggcgcgca gtagctttcc tgtcctgctt tgtggagaag agggcgtggg
    14581 aaaggcactg ctaagtcagg caattcataa tgaaagcgag cgtgctgcag gtccttatat
    14641 cgccgtcaat tgtgagttat atggtgatgc ggcgctggcg gaagaattta ttggtggcga
    14701 tcgcacggac aatgaaaatg gccgtctgag tcggctggaa ctggcacacg gcggcacgct
    14761 gtttcttgaa aagattgaat atctggcggt ggagttacag tctgctttgc ttcaggttat
    14821 caagcagggt gttatcacgc gactggatgc gcggcgttta ataccagttg atgtcaaagt
    14881 gattgccaca acgaccgcgg acctcgcaat gctggtggaa caaaatcgtt ttagtcgcca
    14941 gctgtattac gcgttgcatg catttgaaat taccatcccg cctctgcgta tgcggcgtgg
    15001 cagcattccg gcgctggtga ataacaaatt acgcagtctt gaaaaacgct tctctacgcg
    15061 gctgaaaatt gatgacgatg ccctcgctcg cctggtttcc tgtgcatggc ctggcaacga
    15121 ttttgaactt tacagcgtca tcgagaatct tgctctgagt agtgataatg ggcgcattcg
    15181 cgtcagtgat ttgccggaac atctgtttac cgagcaggca acagatgatg tcagcgccac
    15241 acgcctttcc accagtctgt catttgcgga agttgaaaaa gaggcaatta ttaacgcagc
    15301 ccaggtcaca ggcggtcgca ttcaggaaat gtcggcttta cttggaatcg gccgcactac
    15361 gctgtggcgg aaaatgaagc aacatggcat tgatgcaggg cagtttaagc gccgggtatg
    15421 aaagacagaa acgatttctg atacatcaga gtgatctata tttcattccg gcgcacgcta
    15481 acatttttca gcatcgttta aggacttgtc tatcccgcac ttaaaagctg aagcgatatc
    15541 ctccgttgac ttgcttctga tcaaagaggt taccctgcgt gtaatccgct tcgagataga
    15601 atgtgtgctg tttgttatac tgtgcactga ctcccacgcc attattccag ccattacctt
    15661 tgaaactgta cttctcccgg gagttgttca acagatattc tgtatcccct gaaaactcgc
    15721 ggatagcgcc agtcttcaca tagatattca gctggctatt gcctgcggtg atgtcatatc
    15781 ccagtatcat gctggcacgc cccagcagcg attcgtagtg attcagatgt atattgaggc
    15841 cattactcgc cttcatagcc atctcattct ggtggctgta tgtaagctgg gtttgcggct
    15901 ctatatagaa cccataacca gtaggggaca ggttgaacct ctgcccggct tccagggaga
    15961 tgctcattcc attcgcagtg ccgttggcgt taacgccgtt gttctgactg tccagtacgt
    16021 ggaaactatt tttctggcgc gatgctttta taacgagatc gctgtaaaaa ccgttttgtg
    16081 ccatgtaact ggcgtacatt cccatgtagt ctgaacgtgc ggtaccgtcg cctccgctat
    16141 agtccggcga tgcatgtgtt gagccaatat acagaccgac atacaacggc attacatcag
    16201 agagacgttt atccccacca aactggatac cgctgtaacc catgtcaaag ccgctcagtt
    16261 tgccactggc aaaggagtcc aggcttcccc cataactgcg caaccagata ttaccgtctt
    16321 tactctgatt tcgcaggtca cccatccgtt gcatcagcgt acggttttca acatagttca
    16381 ataagtaacc gacattgaga taattgccgc cagcatctgc ggtcgtggtg ggtttaggcg
    16441 tgggagcggg ttcaggcgta ggatcggggt tgactatggg aggctgagcg ggagttggtg
    16501 tgggttcagg attaggagtc ggttccggaa ctgtcccgga agcgtaaagc tcccagttag
    16561 tgccattttt acgcacatcg tacagatatc cccccaactc aacctgagaa gacgcgctga
    16621 acgaggccgc gccgtcagtg gtttttacca ctgtcagaac ttcatttccc gttgtggcct
    16681 cgctgccctg gttgcggata gccaatacgt gattaccagc actactcccg ctgatattca
    16741 ataaatcccc tttattatta acgccattac cctcgccaac aacatcagca cgcataataa
    16801 aggtactgtt accgctcagg ttctctacgt ttaatgtggc aaatgtgccg gcagttgacc
    16861 cgtggctggc aaaatcgaca gttgaatggc tcagcgccag cgtgtcgaga ttagaattac
    16921 ttgttacgtt ccagacgctg ttattcattg caacgtccag ttttccgcca ttgacattat
    16981 cgctgaggga ggaacctgtc caaaccgacc cagggtgcat atccagattg attagcccac
    17041 ctttggaaag aacgctaccg ttgataagca tacgacccga ggcgttgatg cggctggcgg
    17101 cataaccatc gtcatgttgc gttgcaatgg ccatctggtc gggtgtactc atatcaatga
    17161 ccagatcgct cgtgaggtcg atctggctgt tggtcatggc ataaattccc ctggctcctg
    17221 ccgcgccggt gatagccaaa ctgtctccgg ttattctacc gccgctgagc gcccacaaac
    17281 ccagcgccag actgccatta cgatcaactg taatatcggt attttgcatg ttgacaacag
    17341 ccgttgccgt ctgggccgag gcaccataag aaccgccgga aaagatgctg tttcgttgcg
    17401 ctgccgtgcc agtaaaattg attatcgcgt ctgaaccact ggtgacgagg ccaccgccct
    17461 gcgcggaaga aatatggcta tctgcaccga tagtggttgt accaccacga acttcgacgc
    17521 cattggccgc ggctccagtt acatcaacag tgagtgcatt cgcgctaacc tggccaaagc
    17581 tccagaggcc gtgagcatta tcgccattgg ttttaatggt actgtttgtt ccgagatcga
    17641 caacagagtt tttctgtacg tttatcccca tggcgctgta gccctgaaca tcgattgtca
    17701 ggtctgtagc cgtaaaacgc gcagcaccat tggcgttatt gccgttgaga ccaccgatat
    17761 aaacacctgt acttccatcg gtcgtgattt tacttccgct tccaagatcg acactggtgc
    17821 catagtcatt gatggttagg cctataccgt tcgagttttc aatggtgaat tgagtggctg
    17881 ttagtgttga gctatgccca ataatgatgc cgtcatcgtt actcttaatg gtgctgcctg
    17941 tgcctaagtc agcatgggta tagtcaccaa ttaagttaat gccgatggca gaggtttgac
    18001 caacaacatc tactgtgagt cggttagctg tcagggagct gtttttgccc tgaataatga
    18061 ttccttttga atatccagag gcgtcgttga cattgacggt gacatcatta cccaggttga
    18121 tattgccagg cgtgtttcct gctggggtca ttacaccata gaggtttcct gatggatcgg
    18181 cggtatctcc tgtaattctg tcaccatcat taatagtaat attggtgcca tattgacttc
    18241 ctgtatcagc agctatcagc aggggggaaa agataattgc attcaaacca atagcgccaa
    18301 attttatcag ttgaattgag ggcgaacgga tttttaattc agcgagacga tctgctttgg
    18361 tattcccatt gtgttgtttg atgcccatac cactcctata tagtacccag actatgggtc
    18421 tatggatttt gtcattttgt ttaaatcaat ga
//
LOCUS       DAPHDW010000084        18381 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.084, whole genome shotgun sequence.
ACCESSION   DAPHDW010000084 DAPHDW010000000
VERSION     DAPHDW010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18381
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.084"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            147..1367
                     /locus_tag="RH057_002906"
     CDS             147..1367
                     /locus_tag="RH057_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV3129246.1"
                     /translation="MSIYKIPLPLNILEAARERITWTLNTLPRVCVSFSGGKDSGLML
                     HLTAELARQMGKKICVLFIDWEAQFSCTINYVQSLRELYTDVIEEFYWVALPLTTQNS
                     LSQYQPEWQCWEPDVEWVRQPPQDAITDPDFFSFYQPGMTFEQFVREFAEWFSQKRPA
                     AMMIGIRADESYNRFVAIASLNKQRFADDKPWTTAAPGGHSWYIYPIYDWKVADIWTW
                     YANHQSLCNPLYNLMYQAGVPLRHMRICEPFGPEQRQGLWLYHVIEPDRWAAMCARVS
                     GVKSGGIYAGHDNHFYGHRKILKPEHLDWQEYALLLLNSMPEKTAEHYRNKIAIYLHW
                     YQKKGIEVPQTQQGDIGAKDIPSWRRICKVLLNNDYWFRALSFSPTKAKNYQRYNERI
                     KGKRQEWGILCNND"
     gene            1340..1969
                     /locus_tag="RH057_002907"
     CDS             1340..1969
                     /locus_tag="RH057_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV3129247.1"
                     /translation="MGDTMQQRLTQDLTQFLASLPEDDRIKAINEIRMAIHQVSPFRE
                     EPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLKKSIEIDGFTQPIVVTHTDKNALEIV
                     DGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIRHNRARGRHQITAMSEIV
                     RELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQYSRAWTVK"
     gene            complement(1970..3130)
                     /gene="ybdL"
                     /locus_tag="RH057_002908"
     CDS             complement(1970..3130)
                     /gene="ybdL"
                     /locus_tag="RH057_002908"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HDV3129248.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYSPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSEVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            3239..4327
                     /gene="hcxA"
                     /locus_tag="RH057_002909"
     CDS             3239..4327
                     /gene="hcxA"
                     /locus_tag="RH057_002909"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HDV3129249.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHDFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALS
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNHAEIDKVITHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(4337..4534)
                     /locus_tag="RH057_002910"
     CDS             complement(4337..4534)
                     /locus_tag="RH057_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV3129250.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(4716..6821)
                     /gene="cstA"
                     /locus_tag="RH057_002911"
     CDS             complement(4716..6821)
                     /gene="cstA"
                     /locus_tag="RH057_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HDV3129251.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLALFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQGVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTAGWQKAFSPDAKVGFLAIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKEPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(7002..7415)
                     /gene="entH"
                     /locus_tag="RH057_002912"
     CDS             complement(7002..7415)
                     /gene="entH"
                     /locus_tag="RH057_002912"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HDV3129252.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTRLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(7418..8164)
                     /gene="entA"
                     /locus_tag="RH057_002913"
     CDS             complement(7418..8164)
                     /gene="entA"
                     /locus_tag="RH057_002913"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HDV3129253.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALAFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTPRIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(8164..9021)
                     /gene="entB"
                     /locus_tag="RH057_002914"
     CDS             complement(8164..9021)
                     /gene="entB"
                     /locus_tag="RH057_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HDV3129254.1"
                     /translation="MAIPKLQAYALPESHDIPKNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPVPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(9035..10645)
                     /gene="entE"
                     /locus_tag="RH057_002915"
     CDS             complement(9035..10645)
                     /gene="entE"
                     /locus_tag="RH057_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HDV3129255.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEDFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEIC
                     QFTRQTRYLCAIPAAHNYAMSSPGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVT
                     ALVPPAVSLWLQALTEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENL
                     LLRHPAVIYAALVSMEDELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(10655..11830)
                     /gene="entC"
                     /locus_tag="RH057_002916"
     CDS             complement(10655..11830)
                     /gene="entC"
                     /locus_tag="RH057_002916"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
                     /protein_id="HDV3129256.1"
                     /translation="MDTSLAEEVQQTMATLAPNRFFFMSPYRSFTTSGCFARFDEPAV
                     NGDSPDSPFQQKLAALFADAKAQGIKNPVMVGAIPFDPRQPSSLYIPESWQSFSRQEK
                     QASARRFTRSQSLNVVERQAIPQQTTFEQMVARAAALTATPQVDKVVLSRLIDITTDA
                     AIDSGVLLERLIAQNPVSYNFHVPLADGGVLLGASPELLLRKDGERFSSIPLAGSARR
                     QPDEVLDREAGNRLLASEKDRHEHELVTQAMKEVLRERSSELHVPSSPQLITTPTLWH
                     LATPFEGKANSQENALTLACLLHPTPALSGFPHQAATQVIAELEPFDRELFGGIVGWC
                     DSEGNGEWVVTIRCAKLRENQVRLFAGAGIVPASSPLGEWRETGVKLSTMLNVFGLH"
     gene            12019..12975
                     /gene="fepB"
                     /locus_tag="RH057_002917"
     CDS             12019..12975
                     /gene="fepB"
                     /locus_tag="RH057_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HDV3129257.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAAVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(12979..14229)
                     /gene="entS"
                     /locus_tag="RH057_002918"
     CDS             complement(12979..14229)
                     /gene="entS"
                     /locus_tag="RH057_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HDV3129258.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATPALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPALPPPPQPREHPLKSLLAGFR
                     FLLASPLVGGIALLGGLLTMASAVRVLYPALADNWQMSAAQIGFLYAAIPLGAAIGAL
                     TSGKLAHSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYT
                     MLQTQTPEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGV
                     LLLLVLVELRRFRQTPPQVTASDS"
     gene            14340..15344
                     /gene="fepD"
                     /locus_tag="RH057_002919"
     CDS             14340..15344
                     /gene="fepD"
                     /locus_tag="RH057_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HDV3129259.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAL
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            15341..16333
                     /gene="fepG"
                     /locus_tag="RH057_002920"
     CDS             15341..16333
                     /gene="fepG"
                     /locus_tag="RH057_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HDV3129260.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLETSQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIVTSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAAALLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAADLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            16330..17145
                     /gene="fepC"
                     /locus_tag="RH057_002921"
     CDS             16330..17145
                     /gene="fepC"
                     /locus_tag="RH057_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV3129261.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGTPLVVPLGRTAPSTANS"
     gene            complement(17142..18275)
                     /gene="fepE"
                     /locus_tag="RH057_002922"
     CDS             complement(17142..18275)
                     /gene="fepE"
                     /locus_tag="RH057_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HDV3129262.1"
                     /translation="MSSLNIKQGSDAHFPEYPLASPSNNEIDLLNLIAVLWRAKKTVM
                     AVIFAFACAGLLISFILPQKWTSAAVVTPPEPVQWQELEKTFTKLRVLDLDIKIDRTE
                     AFNLFIKKFQSVSLLEEYLRSSPYVMDQLKEAKIDELDLHRAIVALSEKMKAVDDNAS
                     KKKDEPSLYTSWTLSFTAPTSEEAQTVLSGYIDYISALVVKESLENVRNKLEIKTQFE
                     KEKLAQDRIKTKNQLDANIQRLNYSLDIANAAGIKKPVYSNGQAVKDDPDFSISLGAD
                     GIERKLEIEKAVTDVAELNGELRNRQYLVEQLTKANINDVNFTPFKYQLSPSLPVKKD
                     GPGKAIIVILSALIGGMVACGSVLLRYAMASRKQDAMMADHLV"
BASE COUNT         4343 a         5207 c         4729 g         4102 t
ORIGIN      
        1 tatataacta cagacagcta ctataacttt catctattta ttcacagcgc gaatgaaaaa
       61 taaaacactc tgctttacag tgtttacttt ataattaaca gacttatctc actgatcacc
      121 ctgtaacgtt cagagagcgt cttcagatgt ctatttataa aattcctctt ccgctcaata
      181 ttctggaagc tgcacgagag cgtattacct ggacactcaa caccctaccc cgcgtttgtg
      241 tctcattttc tggcggcaaa gactctggtt taatgctgca tctgacagcc gaacttgccc
      301 gacaaatggg caaaaaaatc tgcgttttgt ttatcgactg ggaggcgcaa ttctcttgca
      361 ccattaacta tgttcagtcc ctgcgcgagt tgtacaccga tgtcatcgaa gagttttact
      421 gggttgcgct cccgcttacg acgcaaaatt ccctttcaca ataccaaccc gaatggcagt
      481 gctgggaacc tgatgtcgaa tgggtacgtc aacctcccca agatgcgata accgaccctg
      541 actttttctc cttttaccag ccaggcatga ccttcgaaca atttgtacgt gagtttgccg
      601 aatggttttc acaaaaacgt ccggcggcga tgatgatcgg catccgtgcg gatgagtcct
      661 acaaccgttt tgtcgccatc gccagtttaa ataaacaacg ttttgccgac gataaaccct
      721 ggactacggc cgcaccaggc ggtcatagct ggtacattta ccccatttac gactggaaag
      781 tggctgatat ctggacctgg tatgcaaatc atcaaagtct ctgcaaccca ctgtataacc
      841 taatgtatca ggcaggcgtt cctctgcgtc atatgcgaat ttgcgaacct tttggcccgg
      901 agcaacgaca aggattatgg ctctatcacg ttatcgaacc ggatcgctgg gctgctatgt
      961 gcgcacgagt cagcggggta aaaagtggcg gcatttacgc cggacatgat aatcatttct
     1021 atgggcatcg gaaaatcctc aagccagaac atttagactg gcaagaatat gcattattgc
     1081 tgctcaatag catgccggaa aaaacagctg agcattaccg caataaaatc gccatttatt
     1141 tgcactggta tcagaaaaaa ggcatcgagg ttccacaaac ccagcaaggg gacattggcg
     1201 cgaaagatat cccctcctgg cggcggatat gcaaagtttt actcaataac gattactggt
     1261 ttcgggcatt atcatttagt cctacgaaag cgaagaacta tcagcgttat aacgaacgga
     1321 taaaaggaaa acgtcaggaa tgggggatac tatgcaacaa cgattaacgc aggatttaac
     1381 gcaatttctc gccagcttgc cggaagatga tcgcatcaaa gcgattaatg aaatccgcat
     1441 ggcgatccat caggttagcc cctttcgtga agagccagtg gattgcgttc tgtgggttaa
     1501 aaacagccaa cttatgccta atgattacaa cccaaacaac gtggcaccac ccgaaaaaaa
     1561 actgctgaaa aaatccattg aaatagatgg ttttacccaa cccattgtgg tcacgcatac
     1621 ggataaaaat gccctggaaa tcgtggatgg ttttcaccgc catgaaattg gtaaaggctc
     1681 atcatcatta aaattacgct taaaaggtta tttgccggtg acctgtctgg aaggtacgcg
     1741 taatcaacgc atcgccgcga ctattcgtca taatcgcgct cgtggacgcc atcaaatcac
     1801 cgccatgtcg gaaatcgtcc gtgaacttag ccagttggga tgggacgata ataaaattgg
     1861 caaagagcta ggaatggaca gcgacgaagt gctgcgcctg aagcaaatta acggcctgca
     1921 agagctgttt gccgaccgtc aatattcccg cgcctggacg gttaaataac taaagctggc
     1981 gcaggcgttc agctgctgcc agcaacgtcg attccttctt ggcaaaacag agacgaatca
     2041 gtttatgtgg gaagggatcg gcgcaaaaca ccgacagcgg aatcgccgct acgccgtgct
     2101 cctgcgtcag ccactggcaa aactcaacat catccagggt agaaacctcg ctgtaatcca
     2161 ccagcaaaaa gtatgtacct tcacacggta aaatctccag ccggctttca tttaaagcat
     2221 tcaccagaat atcgcgcttc tggcgataaa agtccggtaa cgcaagataa tgctcaggtt
     2281 ctgcacgtag catatcagca agcgccagct gtgccggggt attcaccgaa aaggtcagat
     2341 actgatgtac cttgcgaatt tcggcgctga tgggcgctgg cgcaacacaa taacccactt
     2401 tccagccggt catatgatag gtcttgccaa atgaagaaac cgccactgcc cgctcacgca
     2461 gctgcggatg cgccagcaca ctggcatggc cctgttgtga aaagttgatg tgctcgtaga
     2521 cttcatcgct aatgacaaaa atctcgtgcc cggcgatcgc ctgccacaaa gcggcgaaat
     2581 cagcctgctg ccagacagtt gcactggggt tatgcggagt gttgaggatc accagtctgg
     2641 tgcgctcgct taataatgcg gcaaattcct gccagtcaac gcgaaaatgc ggtggttgca
     2701 gtgccatacg cttcactatt cccccagaaa gcgcgatggc gggggagtaa ctgtcatagc
     2761 tgggatcaaa acaaatcact tcatcgccat tgcgcaccag tgcggtaatc gccgcgtata
     2821 acgcttccgt cgcccctgcc gttacggtga tatcgctatc ggcatctggt tgatagccat
     2881 acaaacgttc cgttttctga gcaatcgcct cgcgcaaggc ctgcacgccg gtcatgggcg
     2941 cgtattggtt tgccccctgg gcaacgtggt acgccagccg ctcctgtaaa tagcgcggac
     3001 catcaaaatc aggaaagcct tgcgacaggt taatcgcctg gtgttgctgc gccagcgcgc
     3061 tcatctgggt gaaaatagtg gtgccaagtt gtggaagttt gctttgtgga atcagagggt
     3121 tatttgtcat ttttattgta cctgacgaag ttgcgtgttg aaggcactat aacacgatgc
     3181 tagtatttgg caatcaagac gtttagatgt ctaaataaaa caataaggac aacacaacat
     3241 gcctcacaat cctatccgcg tggtcgtcgg cccggctaac tacttttcac atccaggaag
     3301 tttcaatcac ctgcacgatt ttttcactga tgaacaactt tctcgcgcgg tgtggatcta
     3361 cggcaaacgc gccattgctg cggcgcaaac caaacttccg ccagcgtttg gacttccagg
     3421 ggcaaagcat attttgtttc gtggtcattg cagcgaaagc gatgtacagc aactggcggc
     3481 tgagtccggt gacgatcgca gcgtggtgat tggcgtcggc ggcggcgcac tgctcgacac
     3541 cgcgaaagcc ctcgcccgcc gtctcggtct gccatttgtt gccgctccga cgatcgccgc
     3601 cacctgcgca gcctggacac cgctctccgt ctggtataac gatgccggac aggcgctgca
     3661 ttatgagatt ttcgacgacg ccaattttat ggtgctggtg gaaccggaga ttatcctcaa
     3721 tgcaccgcaa caatatctgc tggcggggat cggtgacacg ctggcgaaat ggtatgaagc
     3781 ggtggtgctg gctccgcaac cagaaacgtt gccgctaacc gtgcgactgg ggatcaataa
     3841 tgcgcaggcc attcgcgacg tattgttaaa cagtagcgaa caggcgctga gcgatcagca
     3901 aaatcaacag ttaacgcaat cattttgcga tgtggtggat gctattattg ctggtggtgg
     3961 gatggtcggt ggtctgggcg atcgttttac gcgtgtggcg gcggctcatg ccgtgcataa
     4021 cggtctgact gtcctgccgc aaaccgagaa atttctccac ggcaccaaag tcgcttatgg
     4081 cattctggta caaagcgcct tgctgggtca ggatgatgtg ctggcgcaat taaccggggc
     4141 gtatcagcgt tttcatctgc cgaccacgct ggcggagctg gaagtggata tcaataatca
     4201 tgcggagatc gacaaagtga ttacccacac cctgcgtccg gtggaatcca ttcattacct
     4261 gccagtcacg ctgacaccag atacgttgcg tgcagcgttc gaaaaagtgg aatcgtttaa
     4321 agcctgacgt gcaaatttag cagcagcgcg cgccgccttt tccaccgtag cgcgcgtcct
     4381 gccgctcacg gaaaaactct tcgtaggtca tcggcgtttg atcgggatgg ttaacccgca
     4441 tatgttcgac atagttgtcg taatcaggca taccaatcat cagcttcgcc gcctgaccta
     4501 aatattttcc ggcttttgcc agtgaatcaa acatcttggc ctccgctaac catgttgctg
     4561 aaagagtgcc ggatgcggcg tgaacgcttt atccggccta caaatgctat caacgcactc
     4621 ggtcagtccc ctcgccccat cggggagagg gttagggtga gggggaaccc tcggcactat
     4681 gccaacattc gccaacatcc cccctcactc tgactttagt gcgcaccttt cgcctgcgct
     4741 acgatctcct caacattttc cggcattggc tcatacggcg tttctttcgc cgttggcttc
     4801 ggctctttca atgccgcaag tgccgtctta atcgagaaca gtgccagaac caccacgacc
     4861 accataaaga agatggttaa cccggcatcc agacggttgt tgaacaccag ttgcgccagc
     4921 tgtgactcag tatactgcga cggaatattg ccgctgtcga tcattgcctg gaacttgtta
     4981 gcaatggcca ggaagccgac tttcgcatcc gggctaaacg ctttctgcca gcctgcggtc
     5041 agggtacaaa tcagtagcca ggccgttggt accagcgcca cccaggcgta acgttgacgt
     5101 ttcatcttga acaacaccac ggcacagagc atcagcgcca tccctgccag catctggttg
     5161 gcaataccaa acagcggcca cagagtgtta ataccgccca gcggatcgac cacgccctga
     5221 tggaggaagt agccccacgc cagtacgcac agcgccgttg ccagcaggtt agcaggcagt
     5281 gaatcggtcc gcttcaggcc aggagacacc acgcccagca gatcctgcaa cataaagcgc
     5341 gcagcacgcg tacccgcatc taccgccgtc agaataaaca gagcttcaaa cagaatggcg
     5401 aagtgatacc agaacgccac atccatcata ccacccagcg caccgtgcag gatgtaagcc
     5461 atccccaccg ccaacgtcgg cgcaccgccc gcacgggaaa tgatcgactg ttcacccact
     5521 tcgctggcaa tctggttgag cgtatctggt gtaatgctaa agccccagct actcaccacc
     5581 tgcgcggcag aagcgaccac atccgccgtc cctgccggag ccagcaccgc cattgggctg
     5641 ttcatggcaa agtaaacgcc cggatcgatg atacaggcgg aaaccagcgc cataatcgcc
     5701 acgaaggatt ccatcaacat tccaccgtag ccgataaaac aagcctgccc ttcgttcgcc
     5761 agcatcttcg gcgtggtgcc ggaagagatc agcgcatgga agccagacac cgcgccacag
     5821 gcgatggtga taaacaggaa cgggaacagg ttaccggtcc ataccgggcc agtgccatcg
     5881 acaaatttgg tcagcgcagg catggtcagc gtcgggcgca taatcaaaat gcctaccgcc
     5941 agaccgacga tcgtcccgat tttcaggaag gtagagaggt agtcacgcgg ggccagcagc
     6001 aaccacaccg gcagcaccgc cgccacaaaa ccgtaaccca ccagcatcca ggtcagctgc
     6061 acgccggtaa agtcaaagta cggtgcccag gtcggacttt ctgccaccca gccgccagag
     6121 ataatggcga aaatcaggaa taccagaccg atgaccgaca cttcaccaat acgccccgga
     6181 cgcagatagc gcaggtagat ccccataaac agcgccagcg gaatggtgaa cgcaacggta
     6241 tacgttcccc acgggctatg agtcagggct ttcaccacga tcatcgccag tactgcaagg
     6301 ataatgacca tgatcataaa gcaggccacc agcgctatca ccccggcggt tggccccatc
     6361 tcttctttga ccagctcacc cagcgatcga ccgtcacggc gcgtagaaac aaacagcacc
     6421 atgaaatcct gcaccgcacc ggcgagaacc accccggcga gcagccagat catccctggc
     6481 aggtaaccca tttgcgccgc cagtaccggc ccaaccaacg gacctgctcc ggcaatggcc
     6541 gcaaaatggt gaccgaacag cactttcttg tccgtcggca catagtccag cccgtcgtta
     6601 tggcgcaccg ctggcgtcat acgcgtcggg tcaaccgcca gcacattttt ggcgatatac
     6661 agcccataaa aacggtaagc gatcagatag atacagaccg acgccaccac aatccacagc
     6721 gcgttgatct gttccccacg atttaaagca atgtatccca gagcaaatgc tcccattaca
     6781 gagagcactg tccagacgag gtatttccct gatttgttca tagttgttat ccgtgtgcgt
     6841 gtccatagag atgttacatt ttgtttctat aacatctttg tacgatttaa caacacggta
     6901 accatgtaaa tcggagttga aaccaatatt taacttaaca atgttaactc gatcactccg
     6961 ttaaccgagt tttcctgcaa tctcatttaa ttctgtcggg ctcatcccaa aactgccgta
     7021 cccagccgac aagtgcagca acgccgcccc tgttcatcga aaacgacgat ttcccagctc
     7081 tgattttgcc gcccaagatg cagcggctgg cagacgccgc gtaccttgcc ttcagacacc
     7141 gggcgatgat gggtggcgtt aagttccgta cccaccacac actgcccgtc acgggtcatc
     7201 ataaatccgg ccatcgatcc cagcgtttca gccagcgccg ccgacgcgcc gccatgcagc
     7261 aggccaaacg gctgatgagt acgggtatca accggcattt cggcttccag cacatcatct
     7321 cccagacggg tatacacaat tcccagatgc gccaccattg tgttatcgct ggtggcgttc
     7381 agttcgtcga gcgttaaatg gcgtttccag atcatgctta tgcccccagc gttgagccgc
     7441 catcgaccac aatatcctgt agggtgatat ggctggcgag gtcagaggcg aggaacaaaa
     7501 tcgtgttggc gatctcttgt ggacgggcga ttttccccag cggaatgccg agtttaaact
     7561 gctcgccaaa gccgcgaata cgctgttctt cggcgtcatc gctcacccac agcgtgcgtt
     7621 gcatatcggt gtcggtggag ccaggcgaaa ccacattaca gcgcacgccg ctacccgcca
     7681 gttccagccc gacgctcaac gccaggcttt tcagcgccgc tttcgatgcg ccataagcac
     7741 tcataccaat acgcggcgtg tgtgcggcgt cggacgccac agtgacaatc gccccgcccc
     7801 gctgacggcg aaactggttc atggtttgct ggaacaggtt aaacgcaccg ccgacgttaa
     7861 ccgcaaaagt ctgctgccag tcctctttac tgagctgatc ggtcgcgccc atgcgcaaaa
     7921 ttcccgccgc attgatcagc acgtccagtc gctccgtttc agctaacagt cgctgacata
     7981 cctgcgccac ctgcccggcg tcggcaacat ccattacttc ggtcgcaaag ggatattgct
     8041 cctgagtgaa cgcttgatca aaacctgtaa ctttcgctcc cgcttcaaca aacgccagcg
     8101 ccgtggcgta gccgatacct ttacctgcgc cggttaccca gacattttta ccgctgaaat
     8161 ccattatttc acctcgcggg agagtagctt ccaccaggcg tcgatggtcg ggtttttcgc
     8221 cagcatgaca aagtcgatat cgccatgcac tttgcgccag cgcgccgcca gcgccatcat
     8281 gcgcaccgaa tccagaccgt agtcgatcag gttgtcgtca tcgaacggtt catcggactc
     8341 gtccagcaac ggcaggataa cctcacgcag cgccgctttg ctggcgggga caggtgctgg
     8401 cagtaattct tcagtcatca ccacccggcc agaccgcccg gccacatatt tcagcgacat
     8461 caaatgctcg tcacggctga aatcggccag cgcatccgcc accataaacg gtttaatatc
     8521 gcgcataaat gcgtcggttg cggtagtcat acagccaatg tgggcatata ccccggtaat
     8581 aatcagctgg ttacgtccac tctctttgag catttgctcc agcggagaac gatgaaacgc
     8641 gctgtagcgc cacttcacca gcaccgtgtc gtcggcatct ggcgtcaggc gatccaccac
     8701 cttttgctgt tccggcgaac gggtcaggcc cggcccccac atatcattca acagcgcccg
     8761 atcttcatcg ctctgctctt tcggctgggc ggtgtaataa accggaatat tgtgctgttt
     8821 gcagtagtcg cgcagcgcag caatattcgc gatcacctgc tccatcatcg ggcagttctc
     8881 gccccagaag ctgacaaaat agtcctgcat atcatggatt aacaacgcgg cacgttgcgg
     8941 ttcaaaggcc cagtcaactt tattcttagg gatatcgtga gactccggca gtgcgtaagc
     9001 ctgtaatttt ggaatagcca tcgtgttctc tccttcaggc tgatgcgcgt gacgccagcc
     9061 actgacgtaa ttgtttttta tcgactttcc cgaccgccgt cagcggaagt gaatctacac
     9121 actccacgcg atccggtaat ttaaattcgg caataccctg ttcacgcagg aaacgacgca
     9181 cctgcaccgc gcgcagcggc tcttttacca ccagataagc gcagcttttt tcgcccatca
     9241 gctcatcttc catgctcacc agtgcggcgt agatcaccgc cgggtggcgc agcagcaggt
     9301 tttcgatctc ttcggcagcg atcttctcac cgccacggtt gatctgatct ttctcgcgcc
     9361 cctgcacggt gatgtacccc tctggatcaa tagagatcag atcgccggaa cagtaaaaac
     9421 cgttggcatc aaaggcgctg gcattgtgct gcgggctttt gtaatagcca cggaaggtgt
     9481 atggaccgcg ggtcatcagg cgtccaactt ccccttgcgg cagtggattt ccttcagcat
     9541 cagcaaccca tacttcatcg tccggacaca ttgggtaacc ctgggtatgg ataattttct
     9601 ccgcgctatc atcaagacgg gtgtagttca ccagcccttc cgccatgcca aacacctgct
     9661 gcaactggca gccaatctca gcgggaatac gcgccgcaag ggtggcagaa agacgtgcgc
     9721 cgccgacctg taacagtttc agcgaggcaa gctgcgcccg gctttcacct tcggtcagcg
     9781 cctgcaacca caggctgact gccggtggca ccagcgcggt gacgttcacc tgatgttttt
     9841 caatcaatgg gaagcaaagc gtggcgctgg gatcggccgc cagaacaacc gttcctccgg
     9901 caagaaagac gcccagcgat cccggcgaac tcatggcgta gttatgagcc gccgggatcg
     9961 cgcacaggta gcgtgtctgt cgtgtgaact gacaaatctc gacgctacga cgcacgctgt
    10021 agtagtagtc gttatgagtg cgcgggatca gtttcggtgt gccggtggtg ccgccggaaa
    10081 gctggaaata ggccacttca tcagcaggtg atggcgtggc agtaaaatcc tcagccggat
    10141 ggttaatcgc atcctgcaag ttatgctcac cgctgtcgtt gtgcagttgc accacgcgaa
    10201 tggaggaatg ttctgtgacg aaagtattga ggaaatcatc cccgctaaac agcgcatgtt
    10261 ggcgatcggc aatcagcaat gcgggttcaa tctggctggc ataggcgttc agttcactac
    10321 gctgatggct gaacagcgcc agcaccggcg caacgcccag tttcagcagc gcgaaaaagg
    10381 taatatacaa ttcagcgacg ttacccagtt gtaccagcgc ggtttcacca ggtttaatgc
    10441 cctgacggcg taaactacac gcgaggttat ccgccgcctg attcagctcc cggtaactca
    10501 actgtcgctc gccgtcgata accgcgatgc tgtcactcgc agcgtggcga gtcagaatgt
    10561 cggtcagcgg caaatcctgc cagtagcctt tttcccgata gcgacgggca aactcttccg
    10621 gccagcgggt gaatggaatg ctcatcctcg ctccttaatg caatccaaaa acgttcaaca
    10681 tggtagaaag tttgacgcct gtttcgcgcc actcacccaa cggtgacgac gcaggcacaa
    10741 tccccgctcc ggcaaacaga cgcacctgat tttcccgcag cttcgcgcag cggatggtca
    10801 ccacccattc gccgttacct tcgctgtcac accaacccac aatgccgcca aacagttcgc
    10861 ggtcgaatgg ctccagttca gcaataacct gggtcgcggc ctgatgcggg aaaccgctca
    10921 gcgcgggggt tggatgcagc agacaggcca gagtcagtgc gttttcttgc gaattcgctt
    10981 taccttcaaa gggagttgcg agatgccaca gcgtcggcgt ggtaatcaat tgtggagagg
    11041 agggaacgtg taactcacta ctgcgttcgc gcagtacctc tttcatcgcc tgagtcacca
    11101 gttcatgttc atggcgatct ttttctgacg ccagcagacg attacccgct tcgcgatcga
    11161 gcacttcatc cggctgacga cgcgcggaac cggctaacgg aatggagcta aaacgctcgc
    11221 cgtctttacg tagcagcagt tccgggctgg cccccagcag gacgccgcca tcagccagcg
    11281 ggacatggaa gttgtaacta accgggtttt gcgcaatcaa ccgttccagc aatacgccac
    11341 tatcaatggc ggcgtcagtg gtgatatcaa tcaaccgtga caacaccact ttgtcgacct
    11401 gcggcgtggc ggtaagtgcg gcagcgcgag caaccatctg ttcaaacgtg gtttgttgcg
    11461 gaattgcctg gcgttctacc acgttcagcg actggctgcg ggtgaaacgg cgggcggaag
    11521 cttgtttttc ctgacgggag aacgactgcc aggattcggg aatatacagc gacgaaggct
    11581 gacgtggatc gaagggaatc gccccgacca tcaccggatt tttgatgccc tgcgctttgg
    11641 catcggcaaa cagcgcggcg agtttttgct ggaagggact gtcgggcgaa tccccgttca
    11701 cagccggttc atcgaaacgg gcgaaacatc ctgacgtcgt aaaactgcgg tacggcgaca
    11761 taaagaaaaa gcgattgggc gcaagtgttg ccatggtctg ctgtacttcc tcagccagtg
    11821 acgtatccat atcatcctcc acaaaatgat aaaggcttta ataatgatta tcatttatat
    11881 tttcggtcgc taacctaaaa gcaaacgcgc actatgtcaa ctcttgaggt aacgcaatct
    11941 gcaactcggg gttgcatctg gttgtggtta caatgaaaat gagaagcatt aacttcatta
    12001 ataacaggaa gctgatttgt gagactcgcc ccgctctacc gcaacgccct tctattaaca
    12061 ggacttttac tttcaggaat agccgcagtt caggccgctg actggccgcg tcagattact
    12121 gacagccgtg gcacacatac actggaaagc cagccgcagc gtattgtttc caccagcgtc
    12181 accctgaccg gctcactgct ggcgattgat gctccggtga tcgccagcgg cgcgaccacg
    12241 ccgaataacc gcgtcgcgga tgaccagggc tttttacgcc agtggagcaa ggtggcgaaa
    12301 gaacgcaaac tgcaacggct ctatatcggc gaaccgagcg ccgaagccgt tgccgcgcaa
    12361 atgccggatc tgattttaat tagcgcaacc ggcggggatt cggcgctggc actgtatgat
    12421 cagctttcca ccatcgcccc gacattaatc atcaattacg acgacaaaag ctggcagtcg
    12481 ctgttaacgc agcttggcga aattaccggg catgagaaac aagcggcaga gcggattgca
    12541 cagtttgata agcaactggc agcggcgaaa gagcaaatca aattaccgcc gcagccggtc
    12601 actgccattg tctataccgc cgctgcacac agtgccaatc tctggacgcc ggaatcagca
    12661 caagggcaga tgctggaaca actcggcttt acgctggcga agttgcccgc agggttaaac
    12721 gccagccaaa gccagggcaa acgccatgac atcattcagc ttggtgggga aaatctggct
    12781 gctgggttaa atggcgagtc actattcctg ttcgccggtg atcagaaaga tgccgatgcg
    12841 atttatgcca atccactgct cgcgcacctg cccgcagtac aaaacaagca ggtttatgcg
    12901 ctgggaaccg agacgttccg cctggattac tacagcgcca tgcaagtgct ggaacgactt
    12961 aaggcgctgt tttaagcatt aactgtcaga cgctgtcacc tgcggcggcg tctggcgaaa
    13021 acgtcgcaac tccaccagca ccagcaataa caacacgccg ataatcaaca aaccaaaacc
    13081 gctcgcgctt gcggaagcaa ccggtgtcat catcgcgccc aaaccaccca gcagcgccgc
    13141 gcctatggca tcgcccgtca cgttctgcgc cgtccacaaa ccgttaatcc gccctaacat
    13201 cgcttccggg gtttgcgttt gcagcattgt atattgcagc aacgaactaa ccgcgctcag
    13261 ccagccgaac agcgccagac aaaccacgcc taaaatccac atcggcatca ggccaaacag
    13321 accaatggcg aggaacgatc ccagcgtgga gagcagcatc aataaccctg gtcgcgcact
    13381 atgtgccagc ttcccgctgg ttaacgcacc aatagccgcg ccgagcggga tcgccgcgta
    13441 gagaaaacca atctgtgccg ctgacatctg ccagttgtca gccagcgccg gatacagtac
    13501 ccgcaccgcg ctcgccatcg tcaataaacc acccagcagc gcaatcccgc ccaccagcgg
    13561 gctggcaagc agaaaacgaa atcctgccag taatgatttc aacggatgct cacgcggctg
    13621 cggtggcggt ggcaacgccg gaaggcttaa caacggtagc aaggtaataa acgtgcccgc
    13681 cgccgccagc ccgtagttcc aggctacgcc accggtcgcc agcaataaac cgccaatcat
    13741 gggcgaaatc accgacccca gacgcacggt caacatggtg atcgccccgg cctgcattaa
    13801 gttttcacgc cctaccagtg ctggtgtcgc cgccaatagc gccgtaacgc caagcgatgc
    13861 gaaaaaacca tcccataaac caagtaaata gattgccagc aatgacggct ccggcagcag
    13921 tgcattaagg cacagtccaa tgaagccaat gccacaggtg ccgcgcgcca gcaaaatcac
    13981 ttttttgcgc tcatagcgat ccgccagcac accgccaacc atcaggccaa caaacatcgc
    14041 gccgccggtc agcgtcaccg aaagccccac ctgccaggta gaatgcgtca tcatctggat
    14101 ctgcaccggc accgcgacgc cgagcaaacc cagagacaca attgagataa aacgagcgag
    14161 gaatactgcg cgaaacgccg ggtgcgtttt caacaggctg aggttaagca gccaggattg
    14221 tttattcatt acaatgcctt gccatcaaat attttaaata cctattctta ggctgcacat
    14281 gctaacatat ccaaataaga tcgataacga taattaattt cattatcatg gaagttcgta
    14341 tgtctggttc tgttgccgtg acacgcgcca ttgccgtgcc cggattgctg ttattactga
    14401 ttatcgcgac ggcattaagc ctgctcattg gggcaaaatc actccccgct tccgtagtgc
    14461 tggaggccct ctccggcacc tgccagagcg ccgactgcac catcgtgctc gacgcgcgac
    14521 tgccgcgcac ccttgccggt ttacttgcag gcggcgcgct tggccttgcc ggggcgttga
    14581 tgcaaaccct cacccgaaat ccacttgccg accccggctt gcttggcgtg aatgccggag
    14641 ccagctttgc cattgtgctg ggtgcggcgc tgtttggtta ctcatccgcg caggaacaac
    14701 tggcgatggc cttcgccggg gcgctggtgg cctcattgat tgttgccttt accggcagtc
    14761 agggcggcgg gcagttaagt ccggtgcgtt taaccctggc gggcgtggcg ctggcggcgg
    14821 tgctggaagg gctgaccagc ggcatcgccc tgcttaatcc tgatgtctac gatcaactgc
    14881 gtttctggca agccggttcg ctggatattc gcaatctaca taccttaaaa gtggtgctga
    14941 tcccggtgct gatcgccgga gcaaccgcgc tattactgag ccgcgcgctg aacagtttaa
    15001 gcctcggcag cgacaccgcg acggcgctgg gcagtcgcgt ggcgcgcaca cagttaattg
    15061 gtctgctggc gattaccgtg ctttgtggta gtgcgacggc ggtagttggc ccgattgcct
    15121 ttatcggcct gatgatgccg cacatggcgc gctggctggt aggtgccgat catcgctggt
    15181 cgctgcccgt cacgctactt gctacccctg ccctgctgct gtttgccgat atcatcgggc
    15241 gggtgattgt gcccggcgaa ctgcgcgttt ctgtggtcag tgcgtttatt ggtgcaccgg
    15301 tgctgatctt cctcgtgcga cgtaaaacgc gaggtggcgc atgatttacg tctctcgccg
    15361 attactcatc acctgtttgc tgcttatttc cgcctgtgtg gttgcaggta tctggggatt
    15421 acgcagcggt gccgtcacgc tggaaacctc gcaggtattc gccgcgctga tgggcgatgc
    15481 gccgcgcagt atgacgatgg tggtcaccga atggcgttta ccacgcgtgc tgatggcgct
    15541 gttgattggc gcggcactgg gcgtcagcgg tgcgattttt cagtcgctga tgcgtaaccc
    15601 gctcggcagc cctgacgtaa tgggctttaa caccggggcg tggagcggcg tgctggtggc
    15661 gatggtgctg tttggtcagg acctgacggc tatcgcgctg gcagcaatgg tgggcggcat
    15721 tgtcacttct ctgttggtct ggctgctcgc ctggcgtaac ggtatcgaca cctttcggtt
    15781 gattattatc ggcatcggcg ttcgcgccat gctggtggcc tttaatacct ggctgttgct
    15841 gaaagcgtct ttagaaacgg cgctaacagc gggtttgtgg aatgccggat cgctcaatgg
    15901 cctgacgtgg gcaaaaactt cgccttccgc acccatcatt atattgatgc tcattgccgc
    15961 cgccttgctg gtgcggcgaa tgcgcttgct ggagatgggc gatgataccg cctgcgcgct
    16021 gggcgtcagc gtcgagcgtt cgcgtctgtt aatgatgctg gttgcagtgg tgcttaccgc
    16081 tgcggcaacg gcgcttgccg ggccgatttc ctttattgct ttagtcgcgc cgcacattgc
    16141 ccgacgcatt agcggcaccg ctcgctgggg gctgacccag gcggcgctgt gcggagcgct
    16201 gttactgctg gcggccgatc tctgcgccca acaactgttt atgccgtatc aacttccggt
    16261 tggcgtcgtt accgtcagcc tcggcggtat ttaccttatc gtcttgttaa ttcaggagtc
    16321 tcgcaaaaaa tgaccgaatc agtagcccgt ttgcgcggcg aacagttaac cctgggatat
    16381 ggcaaatata ccgttgcgga aaatctgact gtagaaattc ctgatggtca cttcacggca
    16441 attatcggac caaatggctg tggtaaatcc acgttactgc gtaccttaag ccgcctgatg
    16501 acgcctgctc acgggcatgt ctggctggat ggcgagcaca ttcaacatta cgccagtaaa
    16561 gaagttgcac gccggattgg tctgttggcg caaaatgcta ccacgccagg tgatatcacc
    16621 gtgcaggagc tggtggcgcg tgggcgttac ccgcatcaac cgctatttac ccgctggcgc
    16681 aaagaagatg aagaagcggt aacgaaagcg atgcaggcca cgggaataac tcatctggca
    16741 gatcaaagcg tggataccct ttctggcgga caacgccagc gcgcgtggat cgcgatggtg
    16801 ctggcccagg aaacggcaat tatgctgctc gacgaaccca cgacctggct ggatatcagt
    16861 catcagattg atctactgga actgttaagc gaactgaacc gcgagaaagg ctataccctg
    16921 gcggcggtgc tgcacgatct taatcaggcc tgtcgttacg ccagccattt gattgcattg
    16981 cgggaaggga aaattgttgc tcagggagcg ccgaaggaga ttgtcactgc tgaactgatt
    17041 gagcgcattt atggtctgcg atgcatgatc attgacgatc cggtagccgg aacgccgctt
    17101 gtggtgccgc tcggacgaac ggcaccttca accgcaaata gttaaactaa gtggtctgcc
    17161 atcatggcat cctgttttct ggatgccatc gcatagcgca ataacacgct accacaagcc
    17221 accatcccgc cgatcagcgc ggaaaggatc acaataatcg ccttacccgg accgtctttt
    17281 ttcactggca atgacgggct taactgatat ttaaacggcg taaaattcac atcgttgata
    17341 tttgcttttg ttaactgctc aacaagatac tgacgattac gtaattcacc gttcagttcc
    17401 gcaacgtcag tgaccgcttt ttcaatttcc agtttgcgtt caataccgtc tgcgccgaga
    17461 gaaatagaga aatcggggtc atctttaacg gcctgaccat tactgtatac gggcttttta
    17521 attcccgccg cgttggcaat gtcgagtgaa taattgaggc gctgaatgtt tgcatcaagt
    17581 tgatttttcg ttttaatgcg atcctgagcc agtttttctt tttcaaactg ggttttgatt
    17641 tccagtttat tacggacgtt ttctagtgac tctttcacca ccaacgcaga gatataatcg
    17701 atatacccgc tcaaaacggt ctgcgcctct tcactggttg gcgcggtaaa acttagcgtc
    17761 caggaggtat acagtgacgg ttcatctttt ttcttactgg cattgtcatc aaccgctttc
    17821 attttttcgc tcaatgcaac aatagcgcga tgcaaatcca gttcatcgat tttcgcctct
    17881 tttaattggt ccatcacata aggtgatgaa cgcaggtact cttccagcaa gctaaccgac
    17941 tgaaacttct tgataaacag gttaaatgct tctgtacgat caattttgat atccagatcc
    18001 agcactcgaa gcttagtgaa tgttttctcc agctcttgcc actgaacagg ttctggaggc
    18061 gtgacaaccg ccgcgctggt ccatttttgc ggcaggatga aagagatcag caagcctgcg
    18121 caggcaaacg caaaaatgac cgccatgacc gtttttttgg cccgccataa aactgcgatt
    18181 agattaagta aatcaatttc attattactg ggcgacgcca gaggatactc aggaaaatga
    18241 gcgtcacttc cctgtttaat attcagtgat gacatggggt ataacctgaa aatgagtcca
    18301 ataggggaaa tctccacggc gctcacttta ccagttggtg ataattatcc gaagctgaag
    18361 tttgtaaatt ccttccactg a
//
LOCUS       DAPHDW010000085        18355 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.085, whole genome shotgun sequence.
ACCESSION   DAPHDW010000085 DAPHDW010000000
VERSION     DAPHDW010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18355
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.085"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            27..633
                     /locus_tag="RH057_002923"
                     /pseudo
     CDS             27..633
                     /locus_tag="RH057_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            736..1239
                     /locus_tag="RH057_002924"
     CDS             736..1239
                     /locus_tag="RH057_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbM family protein"
                     /protein_id="HDV3129263.1"
                     /translation="MGMIGYFAEIDSEKINQLLGSTEKPLMDNIHDTLSGLRRLDIDK
                     RWDFLHFGLTGTSAFDPAKNDPLSRAVLGEHSLEDGIDGFLGLTWNQELAATIDRLES
                     LDRSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNH
                     ALTVIVG"
     gene            complement(1306..2763)
                     /gene="nuoN"
                     /locus_tag="RH057_002925"
     CDS             complement(1306..2763)
                     /gene="nuoN"
                     /locus_tag="RH057_002925"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HDV3129264.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGLMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2770..4299)
                     /gene="nuoM"
                     /locus_tag="RH057_002926"
     CDS             complement(2770..4299)
                     /gene="nuoM"
                     /locus_tag="RH057_002926"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HDV3129265.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMAFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4463..6304)
                     /gene="nuoL"
                     /locus_tag="RH057_002927"
     CDS             complement(4463..6304)
                     /gene="nuoL"
                     /locus_tag="RH057_002927"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HDV3129266.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQSYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFREMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRTLVTSIANSAPGRLLGTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6301..6603)
                     /gene="nuoK"
                     /locus_tag="RH057_002928"
     CDS             complement(6301..6603)
                     /gene="nuoK"
                     /locus_tag="RH057_002928"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HDV3129267.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6600..7154)
                     /gene="nuoJ"
                     /locus_tag="RH057_002929"
     CDS             complement(6600..7154)
                     /gene="nuoJ"
                     /locus_tag="RH057_002929"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HDV3129268.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7166..7708)
                     /gene="nuoI"
                     /locus_tag="RH057_002930"
     CDS             complement(7166..7708)
                     /gene="nuoI"
                     /locus_tag="RH057_002930"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HDV3129269.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7723..8700)
                     /gene="nuoH"
                     /locus_tag="RH057_002931"
     CDS             complement(7723..8700)
                     /gene="nuoH"
                     /locus_tag="RH057_002931"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HDV3129270.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDQVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8697..11423)
                     /gene="nuoG"
                     /locus_tag="RH057_002932"
     CDS             complement(8697..11423)
                     /gene="nuoG"
                     /locus_tag="RH057_002932"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311194.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HDV3129271.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQSKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRHASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSAASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     HVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11476..12813)
                     /gene="nuoF"
                     /locus_tag="RH057_002933"
     CDS             complement(11476..12813)
                     /gene="nuoF"
                     /locus_tag="RH057_002933"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HDV3129272.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12810..13310)
                     /gene="nuoE"
                     /locus_tag="RH057_002934"
     CDS             complement(12810..13310)
                     /gene="nuoE"
                     /locus_tag="RH057_002934"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HDV3129273.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13313..15115)
                     /gene="nuoC"
                     /locus_tag="RH057_002935"
     CDS             complement(13313..15115)
                     /gene="nuoC"
                     /locus_tag="RH057_002935"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HDV3129274.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15209..15871)
                     /gene="nuoB"
                     /locus_tag="RH057_002936"
     CDS             complement(15209..15871)
                     /gene="nuoB"
                     /locus_tag="RH057_002936"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HDV3129275.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15887..16330)
                     /gene="nuoA"
                     /locus_tag="RH057_002937"
     CDS             complement(15887..16330)
                     /gene="nuoA"
                     /locus_tag="RH057_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HDV3129276.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(16961..17899)
                     /gene="lrhA"
                     /locus_tag="RH057_002938"
     CDS             complement(16961..17899)
                     /gene="lrhA"
                     /locus_tag="RH057_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HDV3129277.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYILQKGEPIPLVLLDDPSPFRDMVLATLNKADIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
BASE COUNT         4586 a         5094 c         4726 g         3949 t
ORIGIN      
        1 tatatgaaaa aaaatccttt tgttatatta tttctgattt cactccctct gattattttt
       61 accaaccctg ggtcactgca ttatcgccaa cgccccgaca cgtcagtcct gagcaattag
      121 aaaaaacagt tcgttatctt acacaaactg tgcatccacg taatgccgac aatattgata
      181 atctgaatag gtccgtagag tatataaaag agttctttgt cagtagcggt gccagagtta
      241 ccgcgcaggg cgtccccgat tggctattac gactctgcgc ctggtagcca ggattaccct
      301 tacccgcaat gtcctggctt tatcccgatc ggggggactt tattgcctgg tcggcagaat
      361 acaggatatc aacgccgttc gtcaggtaaa aacggcattg ttatcatccc gggatttatc
      421 tgtttattct atgaatgccc catggtttat tcccggtatt gatttctctg accacctgaa
      481 ttattggcaa cacgatattc ccgccgtaat gattactgac accgcttttt atcgtaataa
      541 acaataccac ttgcccggtg atacagcaga cagattgaat tatcagaaaa tggctcagat
      601 ggtgttataa ccttgttaca caacagtaaa taataaaaat ttctcgaggc gggtccagaa
      661 acagcgacat gtacatttta ttttccctcg tctgttttaa gatgcgaaac ataaaattca
      721 atggaggaaa aataaatggg aatgattggt tattttgcag aaattgactc agaaaagatt
      781 aaccaactgc ttgggtctac tgaaaaacct ttgatggata atatacatga cacgctttca
      841 ggattacgcc gactggatat tgataaacgc tgggattttt tacactttgg gctgacaggc
      901 acctctgctt ttgatcccgc gaagaatgat ccgctgagtc gtgccgtgct tggtgaacac
      961 agcctcgaag atggcataga tggcttcctg ggattgacct ggaatcagga gttagccgcg
     1021 acaatcgacc gactggaatc actggatcga agtgagctgc gtaaacagtt ttccattaag
     1081 cgactcaatg aaatggagat ttatccggga gtgacctttt cagaagagtt agaggggcaa
     1141 ctctttgctt cgatcatgct tgatatggaa aagctgatta gcgcgtatcg acgtatgctg
     1201 cgacaaggga atcatgcgct gacggttatt gttgggtgat ggtgtcaggg caccaaaaaa
     1261 aaccgccgaa catgtcggcg gctttctgac ttacaaactg atagcttaca tcagcggcat
     1321 tgctaaccgc acaatgctaa tcagcggttg tggccataca cccagcacca gtaccaacag
     1381 tgcagagatc agcaccacga taccgcctgc gctgtactgc cagtttgacg gtgcatcgcg
     1441 acccggttgt tccggggcgt gcaggtacag gctcaccgcc acgcgcaggt agtagtaaag
     1501 gccgattgcc gaaccgacaa ccacggcgcc caccagccac cacaagtgtg cctggacacc
     1561 gaccgccagc acgtagaact taccaataaa gcccagcgtc atcggaatac cggccagaga
     1621 cagcatcatc accgtcatca ccgccgcgag gatcgggcga tgccagaaca gaccgcggta
     1681 agagaacagg gaatcagcat ccgggccacg gtacgggctg gacatcaggc tgaccacgcc
     1741 gaacgcgccg aggctgctga acagataacc ggccaggtaa actcctaccg cttccatcga
     1801 catctcgccc gtttgcagcg caatcagcgc caccagcaga tagccgaggt gagagataga
     1861 tgagtaaccg agcagacgtt tgatattggt ctggctcagc gccatcaggt taccgaagat
     1921 gatagaggca aaggcgataa tcgccagcac tacccgaatc gcttcgctgt cacccaccgg
     1981 tgcgtagagg aacagacgca tcaccacacc gaagatagcg attttgctcg ccgtcgccag
     2041 gaaagtggaa accggcgcag gcgcgccctg gtatacgtct ggcgtccaca ggtggaacgg
     2101 caccagagag agtttgaagc cgaggccaac aatcatcagt ccgaaacctg ccagcaacag
     2161 cggctcgttg agcataccgt cgccgaggtt tttacccagc gcgacaaacg acaggtcgcc
     2221 agactgtgca tacaccagcg ccataccaaa cagcaggaaa gaagacgctg cggcagaaag
     2281 gatggtgtat ttgatactgg cttccagcga acgtttctgg cggaaagcgt aaccgaccag
     2341 gccaaacagc ggcaaagaga tcagttcaat accgaggaac aaagacgcca gatggttggc
     2401 attcgccagc aggatcccgc ccagcgcggc aattaacacc aacaggtaga actcatcctt
     2461 gttgtcgtta tagccttcaa gccacgggta ggcgaaagta caggtggcga ggctcgccaa
     2521 caataccagc ccggtgtaaa gcatggcgaa accatcaacg cgcatcagcg gcgtaacgtc
     2581 catagcgccc gcctggccga caaaccagag cgaaaccagc gccgcgttaa gcccaataac
     2641 cgagagcgta gcgttgagga aatgattgcg tcgccacgca atggagagca tcacaaccac
     2701 caccgtcaag ccgacgatca gcaacggtag cagtgcgatc aggttttgtg gagttattgt
     2761 catggcgatt tacggccttg tagtagtaac ggaattaaca aaccactgct ggatattgcc
     2821 aatcgcggag tgcgaggtat ccagaatcgg ctgcggatag aagcccagca gtaccagcag
     2881 caccaccagc aacaggatca taaacagctc acgcagcgac atccctggca gttcctggct
     2941 ggcaatctgg cttttcgctt taccgaagta agcgcgatgc aacatcgcca gcgaataaac
     3001 agatgcaaag accagcccaa aggtagagat aacggtaatc accgggacaa cctggaagct
     3061 gccgaacaga atcataaatt cgccgacgaa gttaccggtg ccaggcatcc caagcgttgc
     3121 caccgcaaag aacagcgaca gtgctggcag ccatttcatc ttgctccaca gaccgcccat
     3181 catgcgcatg tcgcgggtat ggatacgttc ataaagctga ccacaaagaa taaacagacc
     3241 cgccgccgac aagccgtgcg caatcatctg gattaccgcg ccctggtagg ccaactggct
     3301 gccggtgtag atagcaatca gcacgaagcc catgtgggaa accgaggtgt aggcgatcag
     3361 acgtttgata tcggtctggg cgaaggccat ccacgcaccg tagaagatgc cgataacacc
     3421 cagccacata gcaattggcg cgaactctgc cgacgcgttc gggaacagcg gcagggagaa
     3481 acgcagcaaa ccgtaagcgg cagttttcag caagatcccc gcgaggtcaa cggaaccggc
     3541 ggtcggagcc tgggagtgcg catccggcag ccagccatgc agcggaacca ccggcatttt
     3601 gactgcgaag gcgatgaaga agcccagcat taacaggtat tccacaccac tggacattgg
     3661 cgtattcagc agctcttcat agttgaaggt ccagacgccg gtcgcattgt agtgaacaaa
     3721 aaccagcgcc aggatggcaa tcaacatcac caggccactc gcctgggtgt aaatgaagaa
     3781 cttggttgcc gccgtgatac gcgttttacc gtcagaggct ttatgccccc acagtgcgat
     3841 caggaagtac atcggcacca gcatcatttc ccagaagaag aagaacagga acatgtcgat
     3901 ggcaaggaac acgccgataa cgccgcccag gatccacatc aggttgaggt ggaagaagcc
     3961 ctgatatttt tcgatctctt tccacgaaca gagtaccgcc agcacaccga gcagaccggt
     4021 cagcacgacc atcagcagcg acagaccgtc aatggcgaga tgaatagaga taccaaaacg
     4081 cgggatccac ggcatgtcga attcagactg ccactgcgga attccggcgg attgcgtcag
     4141 tgaataaccg ccctgcaacc acagttgcag cgacagcgcc agcgtcaatc ccatggtgat
     4201 cagcgcgatc cagcgcggca ccttgacgcc aaagcgttcg gtctgccagc acaggaagcc
     4261 gccaataaag ggaattaata ttagccaggg tagtaacatg gcgatcttta ttccttgtaa
     4321 aagtcccgtc aggaccggat tttcaacgaa ttctcacgac agaaaattct cttcggactg
     4381 ggggtagtgt cggatgcacc gcgaaggcgg cttatccgac ctacgttctt atatgcccag
     4441 gggcaatccc acaatcctta actcaacgca gtaccatcaa cagtgccagc accacgaccg
     4501 caccgatgct catggatgcc acataccagc gcagatagcc gttctcactt aacagcagac
     4561 ctttacctgc aaagcgggaa aggacagccg ggatgttcat cattgagttc agcggatcgc
     4621 gtttcagcaa ccaggcaata cccaggaacg gcttgacgaa cactttgtca tacagccagt
     4681 caaatcccca ggcgttgtac caccaggtgc ccagcagacg gcccggcgca ctgttggcga
     4741 tggaggtcac cagagtacgt ttacccagcc acagccaggc tgccagcaga atgccgacca
     4801 ccgcgaccac gccagaggta atttccaggg tcaacatgct gccgtgcgcc agttccgtcg
     4861 tttgcggaag cacgccctgc agcggcggta caatcagtgc gccaacgaag gtggaaagga
     4921 tcagcagcac aatcagcggc aggctgtgag ttaccccttt cacggcgtga gcgtgaattt
     4981 gttcttttcc gtggaagacg atgaaaatca tacggaaggt gtagagcgag gtcataaacg
     5041 caccgaccag acctgccacc atcagattga tatgaccatt cgccatcgca cccgcgagga
     5101 tctcatcctt actgaagaag cccgcagtga ccagcggtag tgccgacagt gctgcgccgc
     5161 ccaccaggaa gcagagataa accagcggaa tagatttacg cagaccgccc atcttgaaga
     5221 tgttctgttc gtgatggcag gccagaatga cggaaccgga tgccaggaac agcagcgctt
     5281 taaagaacgc gtgagtcatc aagtggaaaa tcgccgcatc ccatgcctgc acgccaagcg
     5341 cgaggaacat gtagccaatc tggctcatgg tagagtaagc gagaacacgt ttgatgtcgg
     5401 tctgtaccag cgcggcaaaa ccggccagca gcagcgtaac cgccccgaca atacccacca
     5461 gatgcagaac ttccggcgtc atcaggaaca ggccgtgggt acgggcgatc aggtagacac
     5521 ccgcggttac catcgttgcg gcgtggatca gcgcggagac aggcgtcggg cccgccatcg
     5581 cgtcagcaag ccatgtctgc aacggcaact gcgcagattt accgaccgca ccgcccagca
     5641 gcatcagcgt cgcccacatc agcatgttat tgccgtcagc aaagtgcgct ggtgccagtt
     5701 ccaccatttc gcggaagttc agggtgccca gttcgttgta aagaatgaac agtgcgaaag
     5761 cgaggaaaac gtcacccaca cgggtcacga cgaacgcttt cattgccgct gcgccattct
     5821 tcggatcggt gtaatagaaa ccgatcagca gataggagca caggcccacg ccttcccagc
     5881 cgaggtacat cagcagcagg ttatcggcga gaaccagaac taccatgctg gcgatgaaca
     5941 ggttggtgta ggcgaagaag cgagagtagc cctcttcacc gcgcatgtac caggaggcgt
     6001 acatgtgaat aaggaaaccc acaccagtga ccaccgagag catggtcagc gacaggccgt
     6061 ccagcaccag gttaaaaccg atgttaaagt cgcctaccga catccacgtc cacagcggct
     6121 ggctgtatga ctgctcgccg ttagcgaaga aatcaacgcc gataaaggcg gttaccagcg
     6181 ccgccaggcc cacagagcca acgccgacga tcgccgaaac gttttcagac cagcgcccac
     6241 gggagaacgc cagcaggacg aagccaatca atggcaaaat aatggttaag gcaagcatgt
     6301 tcatccgcgc atctcactta ctgaatcgat gttcaggttc tggcgacgac ggtgaagttg
     6361 cagcagcagc gcaaggccga tactcgcttc tgccgccgcg aggctgatgg cgagaatgta
     6421 catcacctga ccgtcggtct gaccccagta gcttccggcg accacaaagg ccagcgcgga
     6481 ggcgttaatc atgatttcca gaccaatcag cataaacagc agattgcgac ggataaccag
     6541 accggttaag ccaagaacga ataagattgc cgcgaggatc agtccatgtt gtaaggggat
     6601 catgcgtgct cctccgtttt tcttttcgcg ctgtcgtctt tacgattgct cagcacttca
     6661 cccgcacgct cttcacgacc gacgtggaag gccacaacca gacccgcgag cagcagcata
     6721 gaagccagtt ccaccgccag tacgtaaggc ccgaacagcg taatacccac tgctttagca
     6781 ctgattggcg taccgtcgat accctgatca ttaacaccaa ggatggcgta aacaatcacc
     6841 accagcatga tggccgacaa aattgccgga ccaatccaca cctgcggttt cagccactgg
     6901 cgttcctgtt cgatttctga accgcccagg ttgagcatca tcaccacgaa cacgaacagc
     6961 accataatgg cacccgcgta gacgataatt tccagcgcac cggcgaagta agcgcccagt
     7021 gagaagaaca ccccggaaat cgccagcagc gaaataatca ggtacagcag tgcgtgtacc
     7081 ggattggtat gggtgatcac tcgcaaggtc gcaagtatgg ctatcaggcc acagatataa
     7141 aaagcgaact ccattgcccc tctccttacg gtaacaggct cttgacgtcg ataggcttgg
     7201 cttcgttctc tgcttcgccc ttatctttgc cgtcgattgc catacctgcc atccggtaga
     7261 agttatattc cgggtatttg cccggaccgg agatcagcag atcctctttt tcgtaaacca
     7321 gatcctggcg cttgtattcc cccatttcga aatccggggt taactgaatc gccgtggtcg
     7381 gacaggcttc ttcgcacaga ccacagaaaa tgcagcgtga gaagttgatg cggaaaaatt
     7441 ccgggtacca gcgaccgtct ttggtttctg ctttttgcag cgagatacag ccgaccgggc
     7501 aggctaccgc gcagaggtta caggctacgc aacgctcttc gccgtccggg tcgcgggtca
     7561 gaacgatacg accacgataa cggggcggca gatagaccgg ctcttccggg tacattcgcg
     7621 tttcgcgttt ggcgaacgcg tgcaggccga tcatccagat actacgaacc tgggtgccga
     7681 aacctactaa caattctttt aaggtcatgg tcttattgcc ccttattgcg cctgccagag
     7741 aatgacagcc gccgttacca gcaagttgat cagcgtcagc ggcaggcaga ttttccagcc
     7801 gaaggacatt acctggtcat aacgcggacg cggtaacgac gcacgaatca aaatgaacat
     7861 catcataaag aacgcggttt tcagcgcgaa ccagatgaat ggcggtaaca acgggccttg
     7921 ccagccaccg aagaacagcg tcaccatcag tgcagagatg gtcacaatcc cgatgtactc
     7981 acccacgaag aacaaaccga acttcatacc ggaatattca atgtggtaac catccgccag
     8041 ttcctgctcg gcttccggct ggtcaaacgg gtgacggtga cataccgcca cgcccgcgat
     8101 ggcaaaggta ataaaaccaa agaattgcgg gatgacgttc cacacatgcg cctggctgtt
     8161 gacgatgtcg gtcatgttga atgaaccggc ctgcgccacc acgcccatca gggaaagccc
     8221 gaggaacact tcgtagctca gggtctgcgc agaagcacgc atcgcaccca gcagcgagta
     8281 tttgttgtta cttgaccagc ccgcaaacag caccgcgtaa accgccagac ctgccatcat
     8341 caggaagaac aaaatcccga tgttcaggtc ggcaaccacc caacccgggc tgacaggcac
     8401 aatcgcaaag gccagcagca gtgaggtaaa ggcaatcatc ggtgccaggg taaagatgac
     8461 gcgatccgag aatttcggga tccagtcttc tttaaagaac attttgatca tgtccgcaac
     8521 cagctggagc gaaccgcccc agccaacacg gttaggtccg taacggttct ggaacagacc
     8581 cagcaggcga cgttcgccaa agctcatgaa tgccccacag gtgacaacca ccagcaggat
     8641 caccaccgct ttgaggatgg tcagcaggat ctcaatcagt tccggtgata tccaactcat
     8701 tgttgtgcct ccttgagatc ctcaagatgc gcgccagcca gcaccggagc aatgccggac
     8761 atacccatcg gcaaacccac ctgccctgcc gtcagccctt cggcgatttc aaccggcagc
     8821 gtgaccgtgt tgccatcgta actaaaggag acgtgtgtac ctgcgttcac acctaacttc
     8881 gcggcatccg ctgggttgag tttgatgtac ggctgcggca tacggctctg gaagaccgga
     8941 gcacgctgtg acaattcatc gctgccaaac aggtggtaat acggcgcgat acgccatttc
     9001 ccgtcctgcg gctggaagcg tgccggtacg ctggtgaagt aatccagacc attttcgctg
     9061 gtttcaaaca gacgcacgcc cggatcgcca aagcgcagtt tgccgcccac ttcgtcctgg
     9121 aatttgttcc acgcctgcgg ggagttccag cccggcgccc aggcaaacgg cacttgcgaa
     9181 cggtgcgcag tcggctggtt gttaccttcc atcgagaagg tgaacatggt gtcaatatcc
     9241 tgcggctgac gcggctcatg aacgctgata ttggcgcgca tggcggtacg accgctgtaa
     9301 cggtgcggtt cacgggccag tttctgccca cgaatacgga atgtcgcatc cggcgcagca
     9361 tctttgatac ctgccagttc cgggattttc gccacaacag cgtcaatcac atggtcgagc
     9421 tgcgtccagt ccacttcacg gctcagcagg gtgctgtgca gcgagtgtaa ccagcgccag
     9481 ctttccagca tgacagtttt gctgtcgtaa taagcaggat cgtaaacctg gaagaaacgt
     9541 tgggcgcggc cttcgttgtt gatcaccgta ccgtcgcttt cagcaaagct ggcagcagaa
     9601 agtaccagat gggcgttttc cataatcgct gtgcgttgat gatcaaccac catcaccagc
     9661 ggcgctttag ccagcgcagc attcacgcgg gtagcggaag cgtgacgatg cagatcgttt
     9721 tccagcacca ccaccgcgtc ggcgcgtccg gtttccagtt cggtcaacgc ttcttcaagc
     9781 gaaccgccac ccataatgcc cagccccatg ctgttgacgg aacgggcaat catggtgata
     9841 ccaacgtcag cgccgcgacc tttcagggct ttcgcgacgt tagccgcagc ctgaatcacc
     9901 tctatgctac cggcgttcgt cccggagata atcaacggtt tcttcgcacc tgccagtgcc
     9961 tgcacgatga cgtcgatttt gctttgcagc tcaggttcga taccgtcaac cgctggcgca
    10021 gagttatcca gcgcatgggc gatggcaaaa cctaaacgcg cctgatcttc aaccggtgcg
    10081 cggtaagtcc acgccgcgat atcatccaga cgggtgtcat caacgttggt aacaaacagc
    10141 ggatgcttcg cacgttgacc gatgttgagg attgccgcaa tctgccagtc agccactttc
    10201 tgtgctgccg ccatttcgcg cgctttacct ttcacagcct gacgcactgc cagcgcgacg
    10261 cgcgcgccgg tctgggtaac gtcttcgccc agcaccagta ccgcatcgta agattcgatt
    10321 tcgcgcagag ccggagtata aatgccgcct tcacgcagca ctttcagcgc cagttgcaga
    10381 cgttcctgct caccgtgagc gataccggtg tagaagtttt cttcgcccac cagttcacgc
    10441 agcgcaaagt tgctttccac gctggcacgc ggagaaccaa taccgatcac tttcttcgac
    10501 tgacgcagaa tatctgccgc gccctgcatt gcctgttcgg cgttgagggt aatgaaatca
    10561 tcgccacgac gctgtactgg ttgacgcgga cgatctttca ggttgacgta accgtaaccg
    10621 aaacgaccac ggtcgcagag gaagtagtgg tttaccgtac cgttgtaacg gttttcgata
    10681 cgacgcagtt cgccgtaacg ttcaccgggg ctgatgttac agccgatgga acattgctgg
    10741 cagatgctcg gcgcaaactg catatcccat ttacggttgt aacgctcgga gtgcgttttg
    10801 tcggtaaata cgccggtcgg gcaaatttcg accaggttac cggaaaattc gctttccagc
    10861 gtgccgtctt ccgggcgacc gaagtagacg ttgtcgtgcg caccgtaaac gcccagatct
    10921 gtaccgtcag cgtaatcttt gtagtaacgc acacagcggt agcaggcgat gcagcggttc
    10981 atttcgtgag agatgaatgg ccccaaatcc tgattacggt gggtacgttt ggtgaaacgg
    11041 tagcgacgga agctgtgtcc ggtcatcaca gtcatatcct gaagatggca gttaccgccc
    11101 tcttcacata ccggacagtc gtgcgggtgg ttggtcatca accactcgac cacgctttca
    11161 cggaactgtt tcgcttcttc gtcgtcaatg gaaataaagg tgccatcgga agccggtgtc
    11221 atacaggaca tcaccaggcg accacgcgtg tcttccgcgt tttggtattg cttcaccgca
    11281 cactggcggc aagcaccgac acttcccagc gccggatgcc agcaaaagta aggaatatca
    11341 aggcccagag acagacaagc ttccagcagg ttgtccgctc cgttgacctc gtattctttg
    11401 ccgtctacat gaattgtagc cattagcatg cttccagttt tctcagtcag agactgagcg
    11461 ttaatcgaaa ttcggttacc agcgctcttt cagcaggttc ggctgaatcc cattaatcaa
    11521 atgggtattg ctgaacggct gtttgattcc cgcctcaaat tcttcgcgga aatatttgat
    11581 ggcgctctgt aacggctcca ctgcaccagg tgcgtgggca cagaaagttt tacccgggcc
    11641 taagaatcga cacagttgct caagtgtttc gatatcgccc ggctggcctt cgccacgctc
    11701 cagcgcacgc agaattttca cgctccacgg cagaccgtcg cggcacggcg tacaccagcc
    11761 gcaggactca cgggcgaaaa actcttccag gttacgcacc agcgacacca tgttgatctc
    11821 atggtcaacc gccatcgcca gcgccgtacc cagacggctg cccgctttac cgatactttc
    11881 gaattccatc ggcagatcaa ggtgcgcttc ggtcaggaag tcggtgcccg cgccgcctgg
    11941 ctgccaggct ttaaatttca gaccatcacg cataccaccg gcgtaatctt cgaggatctc
    12001 gcgtgcggtg gtgccgaacg gcagttccca cagtcccgga tttttcaccc gaccggagaa
    12061 gcccatcagc ttggtgccag catctttact tttcgagatg ttctgatacc actccacgcc
    12121 gttagcgagg atcgccggaa cgttacacag ggtttcgacg ttgttgacac aggtcggttt
    12181 gccccatacg ccggaggttg ccgggaaggg gggtttcgag cgtgggttag cacgacgccc
    12241 ttccagggag ttgattaacg ctgtttcttc cccgcagatg tagcgccctg ccccggtatg
    12301 gacgaacagt tcgaagtcga aacctgttcc cataatgttt ttgccaagca agcccgcttc
    12361 agtggcttcg gcaatggcgc ggcgcagatt tactgccgct tcgatatatt cgccacgcag
    12421 gaagatgtag ccacggtaag ctttcagcgc aaacgcggag atgagcatac cttccaccag
    12481 caggtgcggc agttgctcca tcagcaggcg gtctttatag gtgcctggct ccatttcatc
    12541 ggcgttacac agcaggtaac ggatgttcat ggattcgtct ttcggcatca ggctccattt
    12601 caggccggta gaaaagcccg cgccaccgcg ccctttcaga ccagcgtctt ttacctgatt
    12661 aacgatttcg tccggagaca gcccggtcag cgccttacgc gcgccttcgt aaccgttttt
    12721 gctgcgatat tcgtccagcc acactggctg tttgtcatcg cgcagacgcc aggtcagcgg
    12781 atgcgtttcg ggagtacgga taatgttttt catttatacc gctccagcag ttcagggatc
    12841 gcttccgggg tcagatgcgc gtgagtatcc tcatcgatca tcatgtttgg ccctttatca
    12901 cagttcccca ggcagcaagt tggcagcagc gtaaagcggc catcaaatgt cgtttgccct
    12961 ggtttgatgt tcagcttttt ctcgagcgcc gcctgaatac cctgataccc gttgatatga
    13021 cagaccacgc tgtcgcaata acggatcaca tggcgaccaa ccggctggcg gaagatctga
    13081 ctgtagaacg ttgccacacc ttcgacgtcg cttgccggaa tacccagcac atcggcgatc
    13141 gcgtggatcg caccatccgg cacccagcca cgctgcttct gaacgatttt cagcgcttca
    13201 atggacgccg cacgcgggtc ttcgtagtgg tgcatctcgt gctcaatcgc ttcacgctct
    13261 gccgcactca gctcaaaagc ctcggtttgt ggttgttgat tctcgtgcat aattagcggt
    13321 ccacatctga cataacaaaa tcgatactgc ccagataaac aatcaggtca gacaccaggc
    13381 tgccgcggat cgccgccgga atttgctgca aatgcgcata actcggcgtg cggatacgag
    13441 tgcgataact catggtgcta ccgtcgctgg tcaggtagta actgttgatc cccttggtcg
    13501 cctcaatcat ctggaaagat tcattggcag gcatcaccgg cccccacgac acttgcagga
    13561 agtgggtgat cagggtttcg atatgttgca gcgtgcgctc tttcggcggc ggcgtggtca
    13621 gcgggtgatc cgctttgaac gggccttccg gcatgttgtt gaggcactgc tcaagaatgc
    13681 gcagactctg gcgcagctct tccactttca gcattacgcg ggtgtagcag tcagaaacac
    13741 caccaccaac cgggatttca aagtcgaagt tttcatagcc agaataagga cgcgccttac
    13801 gcacgtcgaa atcgatcccg gtagcacgca ggcccgcgcc agtggtgccc cactccagcg
    13861 cctctttcgc gccataggcg gcaacgccct gggaacgacc tttcagaatg gtattttgca
    13921 gcgccgcttt ctcgtaagac gccagacgtt tcggcatcca gtcgaggaac tcacgcagca
    13981 ggcgatccca gccgcgcggc aggtcgtgcg ctacgccgcc aatacggaac cacgccgggt
    14041 gcatacggaa acccgtgatc gcttccacca gatcgtaaat tttctgacga tcggtaaagg
    14101 cgaagaacac gggcgtcatt gcgccgacgt cctgaataaa ggtcgagatg tacagcaggt
    14161 ggctgttgat acggaacagt tcggagagca taacgcgaat gacgttaacg cgatccggca
    14221 cggtgatccc ggccagtttc tctaccgcca gcacgtaagg catttcgtta acgcagccgc
    14281 cgaggtattc aatacggtcg gtatacggaa tgtagctgtg ccaggactgg cgctcgccca
    14341 ttttctccgc accacggtgg tggtagccga tgtctggtac gcagtcgaca atctcttcgc
    14401 cgtcgagttg taaaacgata cggaaagccc cgtgcgccga cgggtggttc ggaccgaggt
    14461 tgaggaacat gaagtcctcg ttttcggtgc cgcgcttcat cccccactct tccggtttga
    14521 aggtcagggc ttccatctcc agatcctgtt tggctttggt cagctcaaac ggcgagaatt
    14581 cggtagcgcg cgccgggtaa tctttacgca gcgggtgacc tttccaggtt tgcggcatca
    14641 tgatgcggcg caggttcggg tgaccgtcga aagtaatgcc aaacagatcc caggtttcac
    14701 gctcatacca gttagcgttc gggaacagtt tggtgaaggt cggtacgtgc aggtcgtttt
    14761 ctgccagcgc caccttcagc atgatgtcgc ggttacgatc gatagaaatc agatggtaga
    14821 aaacggaaaa atccgcggca ggtaaccctt cgcggtgtgt gcgcagacgt tcgtccatgc
    14881 cgtgtaagtc aaacagcatg acgtaaggtt tcggcagttt ctttaagaaa tcgccaactt
    14941 ccagtaattg ttcacgcttg atccacacaa cgggaacccc ggtgcgagtc gcctgaacag
    15001 taaaggcatc cggcccaaaa cggttgcgca gttcgccaat caccggatca tcaagatgat
    15061 cgcgggtctg ccaggcgggt tcttgcgcgg ttaagtcggt catattgttc accattgcaa
    15121 atggtccgtg gtgactgtcg gcgcagtaag cttcgcgaaa tagatttagg aataagcgaa
    15181 gaaaaatccc tttgccgaca ggcgcaaatt aaatctcgtc aggtgtacgc aggttggtta
    15241 cggcaatacg ttcaccgcgc ttgcgttcgc gctctgattg catattggcg cgataaacgc
    15301 cctgatcgcc aaccacccag gagagcggac gacgttcttt gccgatagat tcctgcaaca
    15361 gcatcagtgc ctgcatgtac gcttcaggac gcggcgggca gcccgggata tacacatcaa
    15421 ccgggatgaa tttatcgacg ccctgcacaa cggaataaat atcgtacata ccaccagagt
    15481 tggcacaggc acccattgag ataacccatt ttggttccag catctggtca tacagacgct
    15541 gaataaccgg tgccattttg gtaaagcagg ttcctgcaac caccatcagg tcagcctgac
    15601 gcggcgaagc acgcaatact tctgcgccaa aacgcgccac gtcatgcacc gcggtaaacg
    15661 aagtcaccat ctcaacgtaa cagcaggaaa gaccgaagtt atacggccaa attgagtttt
    15721 tacgacccca gttaaccatg tcattgagct tgcccataaa cacgtttttg ttaacttctt
    15781 gctccagagg gtcggttacg atctcctgct tttgcagggg gtaacggtcg ttctcaccgt
    15841 tgggatctat gcgggtgagc gtataatcca tcttaatgcc tcgcggttag cgttgacgat
    15901 tagcgatact gttcgtttcc gggttcatac gctcgcggcg tgaacgcgcg ggcgtccagt
    15961 ccagcgcgcc aatacgcacc agataaacca gacctgccag taacacaaaa ataaaaattg
    16021 cagcttccac aaagcctacc cagccgcttt cgcggataga ggttgaccat gcgaacagat
    16081 acagcgcttc aacgtcgaag ataacgaaga acatggccac cagataaaac ttggcggaca
    16141 ggcgtaagcg ggcggagccg accgagtcga taccggattc aaacggcacg tttttcgacc
    16201 tcgcgcgtgc gcgaccgcct aaaaaccaac cgcctaccag catcaggcaa cacaggccaa
    16261 tggcaacgat aagaaagata gcgaatgccc agtgatgagc gatgacttca gtggatgttg
    16321 acatactcat tgcttactca tcaaaagtag cgccagattc actgctcttc acggcagatg
    16381 gacgccacat cgattcatgg ggaggaataa aaaaaacctt acaatcactg tagaaattct
    16441 tttatacagc taattgatgt ggtcttttac tcctttctat aaccttttgt caactttaac
    16501 aaaagtttct tcacattagt ttacataata tcaacaccat tagcatttaa tgccctttca
    16561 ccccagatcc ttgaccactc caggataatt agatgttgtt gaatcgtgtc cgttgtgaag
    16621 caatggaaaa aatacgggtc tattttgaca ggaatttgtg tcgattcctc cccccaaaag
    16681 agagtatttt cttgatctgt gacacgcttt tgtcattcca taacaaaaac gcagcaacaa
    16741 atttacgtat tttttaacat cattgtagca ggtgattttt ttcaggcgat tatttgtgcg
    16801 ttcgggacgt gaatctctgg tgattgaaaa atgaacagtt ttgtacgttc tgcactatgc
    16861 gaggaaggat ttttactaaa aaaaagccgc tggggtttaa aacaccccca gcggctcgtt
    16921 ttttacacta ttgtctcagg aattatctat cgtccgtcga ttactcaata tccctttcaa
    16981 tcaacaatga atcatcccct tccggagcag acattgggct gtattgccac ggattatggt
    17041 agctctccat cgcctgatag atcacctggg ccagctcatt attactggac gggtcatagc
    17101 acagcaaata ttcggtatca ggtagcggcg gtaagccatc tactccactc agcacacgca
    17161 ggtccgggct catcatttca accggccttg ccgtcacgcc aagaccggct ttcactgccg
    17221 cacgaacggc cggaagcgtc gaggcgacat aagccagtcg ccatggaata tctgctttat
    17281 taagcgtcgc cagcaccata tcgcgaaacg ggctaggatc atccagcaat acaagaggga
    17341 tcggctcgcc tttttgcaat atgtattccg ctgcgcagta ccagtgtgtt ggcgaggtac
    17401 gcaggttcaa tgccttaaac gctgaaggac gatgggtggt taccatcaaa tccacttcct
    17461 gggattccag catttctgcc atataggcat tacgtttaac gcggacatcc agcgctaatt
    17521 tcggataaac cgaactcacg cgatttaaca ggaaaggtaa gatcgtatcg gcagattcat
    17581 ctgaagcacc gatagttaac acgccctgaa gattactgaa cattaatgat gagcaggcct
    17641 catcattaaa acgcaggatt ttcctggcgt aaccaagaag ttgaatgcca tgttcagtta
    17701 acagtttgtt gcgaccgtga cgagcgaaca gttctttccc aacgagttgt tccagacgct
    17761 gcatttgctg acttacggcg gactgagtac gacacacagc ggcagctgcg gcagcaaaag
    17821 tgttcagatc ggcaacagca acaaatgttc tcagcagatc gaggtcgagg ttaattatcg
    17881 gacgatttgc acttatcata tattatcact tactggcggc tcatactgag ctggttaatg
    17941 ctgtgcacac acaaacaagc aattccattt gtaatgtgcc tccctggcag tttcatccgg
    18001 aacccggacg aaagtaaaaa tgcatatgag ttgcactaaa aaagcgactc acattgttcc
    18061 gttataatgc ctgaagtaga tcacagaata tatcttcagg gatcgcatat ctattaagtt
    18121 actcactctt ttctatttat gacatgcgcg tgtttgtata aatgtaaatg tgagtccttg
    18181 ttccactctc gtgcagcatc gctggtcata cgcgaacacg tacaaacagc aatggtgagg
    18241 cggcatcaag agcagggatc cgtcatttat gcgagcattt taccccaaaa accttttatt
    18301 tataagggtc attgcgaatt atctgatgca aagttatgtt atgttaggca aagta
//
LOCUS       DAPHDW010000086        18351 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.086, whole genome shotgun sequence.
ACCESSION   DAPHDW010000086 DAPHDW010000000
VERSION     DAPHDW010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.086"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..590)
                     /gene="rsmG"
                     /locus_tag="RH057_002939"
     CDS             complement(<1..590)
                     /gene="rsmG"
                     /locus_tag="RH057_002939"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HDV3129278.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGER"
     gene            complement(654..2543)
                     /gene="mnmG"
                     /locus_tag="RH057_002940"
     CDS             complement(654..2543)
                     /gene="mnmG"
                     /locus_tag="RH057_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HDV3129279.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     LYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASQHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            complement(2922..3365)
                     /gene="mioC"
                     /locus_tag="RH057_002941"
     CDS             complement(2922..3365)
                     /gene="mioC"
                     /locus_tag="RH057_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HDV3129280.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEAGFTTETLHGPLLEDL
                     SASGIWLIISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGAIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            complement(3455..3913)
                     /gene="asnC"
                     /locus_tag="RH057_002942"
     CDS             complement(3455..3913)
                     /gene="asnC"
                     /locus_tag="RH057_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HDV3129281.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4065..5057
                     /gene="asnA"
                     /locus_tag="RH057_002943"
     CDS             4065..5057
                     /gene="asnA"
                     /locus_tag="RH057_002943"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HDV3129282.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQFSTLKSTVEAIWAGIKATEAAVSEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLEL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRENVPSLL"
     gene            complement(5062..6513)
                     /gene="viaA"
                     /locus_tag="RH057_002944"
     CDS             complement(5062..6513)
                     /gene="viaA"
                     /locus_tag="RH057_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HDV3129283.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQLIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMEAFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVIERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(6507..8003)
                     /gene="ravA"
                     /locus_tag="RH057_002945"
     CDS             complement(6507..8003)
                     /gene="ravA"
                     /locus_tag="RH057_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HDV3129284.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFMLRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTVIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERNALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSAQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEASLQDVGAQIRQAQQC"
     gene            8227..10095
                     /gene="kup"
                     /locus_tag="RH057_002946"
     CDS             8227..10095
                     /gene="kup"
                     /locus_tag="RH057_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HDV3129285.1"
                     /translation="MSTDNKQSLPAITLAAIGVVYGDIGTSPLYTLRECLSGQFGFGV
                     ERDAVFGFLSLIFWLLIFVVSIKYLTFVMRADNAGEGGILTLMSLAGRNTSARTTSML
                     VIMGLIGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVLTLLFMIQKH
                     GTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLEYKTVSFIAL
                     GAVVLSITGVEALYADMGHFGKFPIRLAWFTVVLPSLTLNYFGQGALLLKNPEAIKNP
                     FFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMRIIHTSEMES
                     GQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILSTTVARQNWHW
                     NKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWKSERFRLLRR
                     MHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNKVLHERVILL
                     TLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGLEGLSCRMME
                     TSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIELGTQVEI"
     gene            10262..10681
                     /gene="rbsD"
                     /locus_tag="RH057_002947"
     CDS             10262..10681
                     /gene="rbsD"
                     /locus_tag="RH057_002947"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HDV3129286.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            10689..12194
                     /gene="rbsA"
                     /locus_tag="RH057_002948"
     CDS             10689..12194
                     /gene="rbsA"
                     /locus_tag="RH057_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
                     /protein_id="HDV3129287.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYARDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWKTMYAEADKLLAKLNLRFKSDKLVGDLSIGDQQMVEIAKVLSFES
                     KVIIMDEPTDALTDTETESLFRVIRELKSQGRGIVYISHRMKEIFEICDDVTVFRDGQ
                     FIAEREVASLTEDSLIEMMVGRKLEDQYPHLDKAPGDIRLKVDNLCGPGVNDVSFTLR
                     KGEILGVSGLMGAGRTELMKVLYGALPRTSGYVTLDGHEVVTRSPQDGLASGIVYISE
                     DRKRDGLVLGMSVKENMSLTALRYFSRAGGSLKHADEQQAVSDFIRLFNVKTPSMEQA
                     IGLLSGGNQQKVAIARGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKADGLSIIL
                     VSSEMPEVLGMSDRIIVMHEGHLSGEFTREQATQEVLMAAAVGKLNRVNQE"
     gene            12199..13164
                     /gene="rbsC"
                     /locus_tag="RH057_002949"
     CDS             12199..13164
                     /gene="rbsC"
                     /locus_tag="RH057_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HDV3129288.1"
                     /translation="MTTQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALALGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            13189..14079
                     /gene="rbsB"
                     /locus_tag="RH057_002950"
     CDS             13189..14079
                     /gene="rbsB"
                     /locus_tag="RH057_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
                     /protein_id="HDV3129289.1"
                     /translation="MNMKKLATLVSAVALSATVSANAMAKDTIALVVSTLNNPFFVSL
                     KDGAQKEADKLGYNLVVLDSQNNPAKELANVQDLTVRGTKILLINPTDSDAVGNAVKM
                     ANQANIPVITLDRQATKGEVVSHIASDNVLGGKIAGDYIAKKAGEGAKVIELQGIAGT
                     SAARERGEGFQQAVAAHKFNVLASQPADFDRTKGLNVMQNLLTAHPDVQAVFAQNDEM
                     ALGALRALQTAGKSDVMVVGFDGTPDGEKAVNDGKLAATIAQLPDQIGAKGVETADKV
                     LKGEKVQAKYPVDLKLVVKQ"
     gene            14205..15134
                     /gene="rbsK"
                     /locus_tag="RH057_002951"
     CDS             14205..15134
                     /gene="rbsK"
                     /locus_tag="RH057_002951"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HDV3129290.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPALVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            15138..16130
                     /gene="rbsR"
                     /locus_tag="RH057_002952"
     CDS             15138..16130
                     /gene="rbsR"
                     /locus_tag="RH057_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HDV3129291.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(16096..17523)
                     /gene="mdtD"
                     /locus_tag="RH057_002953"
     CDS             complement(16096..17523)
                     /gene="mdtD"
                     /locus_tag="RH057_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HDV3129292.1"
                     /translation="MSDKKKRSMAGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGGAMMMPVARLALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARHTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRVYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSKPNRVPSESE"
     gene            complement(17546..18238)
                     /locus_tag="RH057_002954"
     CDS             complement(17546..18238)
                     /locus_tag="RH057_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HDV3129293.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWMTEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIVQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         4404 a         4549 c         4807 g         4591 t
ORIGIN      
        1 cgttcgccat ccagggctgg aacctgaagt ttaaccactg attcgacctg atattcttcg
       61 ggcaacaaag cgatttcatc ttccggcatt tgccctttca gcgcgtagaa acggccttgc
      121 tcaccaggaa gatggtggca ccagctcacc atatcgttca gagaggcaaa agcgcggcta
      181 attacgccat caaatggcgg ctctgaagga aactcttcta ccctgctctg tactggttca
      241 atattctcca gtttaagctc atgttgcacc tgacgaagga aacgcacgcg tttaccaagg
      301 ctatccaaca gagtgaaatg ggcttcagga cgcacgatag agagtggaat gcctggcagt
      361 cctggtccgg tgccgacatc gataaaccgt tcaccttgca gatacggtgc caccacaatg
      421 ctatcgagaa tatggcgtac cagcatctca ttaggatcgc ggaccgaagt caggttgtac
      481 gctttgttcc atttatgcag catattcacg taggcaataa gctggttttt ctggtgatcg
      541 gtaagcgaaa tacctgcgtc tttcagcagt aaggagagtt tgttgagcac ggtgattacc
      601 tgttcttgat gcgttgcctg gtaagcgggt gcttaccagg catttttaat gcgttatgcg
      661 ctacgacgca gcataccctg ttttttcagc cacaccagca gaatggagat ggccgcaggc
      721 gtgacgccag aaatacgcga agcctggccg atagaggctg gtttgtgatc gttaagtttg
      781 gcgatcactt cgttagaaag accggatacc tggcggtaat ccagtgtcgc gggtagcagg
      841 gtgttctcgt tacgcagctg cttttcgatc tcatcttgct ggcgcgcgat ataaccttcg
      901 tatttaacct gaatctcaac ctgttccgcc gcctgttcgt ctgtcaacgc aggggcaaac
      961 ggcgtcagcg tggttaattt ttcataagtc atttccggac gacgcagcag atcttcacca
     1021 ctggcttcac gggaaagcgg cgcagtcagg tgagcattca cttcggctgc agcttccgcc
     1081 gacggggtta cccaggtcga tttcagacgc tgacgctcac gctcgatatt ctcaagtttc
     1141 tcgttaaagc gcgcccaacg ttcgtcatcc accaggccca gttcacgacc gatttcagtc
     1201 aaacgcagat ccgcattatc ttcgcgtagc atcagacgat attctgcgcg cgaagtaaac
     1261 atacgatacg gttctttggt tcctaaagtg cacaggtcat caactagtac gccgagatac
     1321 gcctgagaac gtgccggagc ccaaccttct ttgtcagcag acagacgggc agcgttaaga
     1381 ccggccagca aaccttgcgc agcggcttct tcgtaaccgg tagtgccgtt aatctgacca
     1441 gcaaagaaca gcccctggat aaacttgctc tccagcgtcg gtttcaggtc gcgaggatcg
     1501 aagaagtcat actcaatggc ataacccgga cgcacgatct tcgcgttttc catcccctgc
     1561 atagagcgga cgatttgcat ctgcacatcg aacggcaggc tggtggagat accgttcgga
     1621 taaatttcat tagaggtcag tccttccggt tcaaggaaga tctgatgctg atttctgtcg
     1681 gcgaagcgca tgactttgtc ttcgatcgac gggcagtagc gtgggccgac accttcgatc
     1741 acccctgcat acattgggct acgatcgagg ttactgcgga tcacatcatg ggttttctcg
     1801 ttggtatgag tgatataaca cggcacctgc tggggatgct gggacgcatt gcccataaac
     1861 gagaataccg gcattgggtt atcgccatgc tgttgcgcca gcacactaaa gtcgatagtt
     1921 cgagcatcaa tacgcggtgg tgtcccggtt ttcagacgac caacgcgcag cggcagttca
     1981 cgcaaacggc gagaaagcgg aatggacggc ggatcaccag cacggccacc gctgtaatta
     2041 tccagaccga tatgaatttt accgtcgagg aacgtcccaa cggtgagcac gacggctttg
     2101 gcacggaact tcagtcccat ttgggtaaca gcaccgacca cgcgatcgtt ttcgacaata
     2161 agatcttcaa ccgcctgctg gaagatcatc aggttcggtt ggttctccag cgccgtacgt
     2221 accgcctgac ggtagagcac acgatccgcc tgagctcggg tagcgcgaac cgccggtcct
     2281 ttgcttgcgt ttagtatcct aaactggata cccgcttgat cgatcgcttt cgccatcaga
     2341 ccgccgagtg catccacttc ttttaccaga tgtcccttcc caataccgcc gatcgccggg
     2401 ttgcagctca tctgccccag agtgtcgata ttgtgtgtca aaagcagagt ctgttgaccc
     2461 atacgcgccg cggccatcgc ggcctcggtg cctgcatgac ccccgccaat gatgatgacg
     2521 tcaaaaggat ccggataaaa catggtgatt gcctcgcata acgcggtatg aaaatggatt
     2581 gaagcccggg ccgtggattc tactcaactt tgtcggcttg agaaagacct gggatcctgg
     2641 gtattaaaaa gaagatctat ttatttagag atctgttcta ttgtgatctc ttattaggat
     2701 cgcactgccc tgtggataac aaggatccgg cttttaagat caacaacctg gaaaggatca
     2761 ttaactgtga atgatcggtg atcctggacc gtataagctg ggatcagaat gaggggttat
     2821 acacaactca aaaactgaac aacggttgtt ctttggataa ctaccggttg atccaagctt
     2881 cctgacagag ttatccacag tagatcgcac gatctgtata cttatttgag taaattaatc
     2941 cacgatccca gccattcttc tgccggatct tccggaatgt cgtgatcaag aatgttgatc
     3001 ttcagtgttt cgcctgtctg ttttgcaccg gaatttttga gttctgcctc gagtttatcg
     3061 atagccccac aaaaggtgtc atattcacga ctgccaatac cgattgcgcc aaagcggacc
     3121 gcagaaagat cgggcttctg ttcctgcaat gcttcataga aaggagaaag gttgtccgga
     3181 atatctccgg caccgtgggt ggagctgata atcagccaga tccccgaggc agataaatct
     3241 tccaacagcg gaccgtgcag cgtttcggtg gtaaaacccg cctcttccag cttttcagcc
     3301 aggtgttctg ctacatattc ggcaccgccg agggtgctgc cgctgataag agtgatatct
     3361 gccataaacc gccaccttta ttaagagtgg cgtattgtac gctgtgaacg cgttgggatc
     3421 tacctgtgga aaagtatggg attaaaaaag ccgattatgg cttgatggta cgcatgatcg
     3481 ggttttgcag gacgatcaat gtctcggtgg actgaatttc atcaattgtt tggatcttgt
     3541 tgataagtac atgctggaga gcgtcgatcg aacggcacat cacttttata aagatgctgt
     3601 agtggccggt tgtgtaatag gcttcagtga cttcatcaag gctttccagc tttgccagcg
     3661 cggaagggta gtctttggcg ctctttaata taatgccgat aaagcagcct acgtcataac
     3721 cgagctgctt cgggctgaca tcaatacgcg ccccggtaat gatccccgcc tgcttcattt
     3781 tctctactcg aacgtgaatc gtccccggac tgacgccaaa ttgtttcgcc agttcggcgt
     3841 aagcggtgcg cgcattgccc attaatgctt ccaggatgcc acggtccaga ttgtcgatca
     3901 gataattttc cataggattt tcttatgcag attgatgatt cattctattt tagccttctt
     3961 ttttaatgaa tcaaaagtgt gttaggcttt ttattgaatg attattgcat gtgtgtcggt
     4021 ttttgttgct taatcataag caacaggacg caggagtata aaaaatgaaa accgcttaca
     4081 ttgccaaaca acgccaaatt agcttcgtga aatctcactt ttctcgccaa ctggaagaac
     4141 gtctggggct gatcgaagtc caggcgccga ttcttagccg tgtgggggat ggcacgcagg
     4201 ataacttgtc gggctgtgaa aaagcggtgc aggtaaaagt gaaagctctg cctgatgccc
     4261 agttcgaagt ggttcattca ctggcgaagt ggaaacgtca gaccttaggg caacacgact
     4321 tcagcgcggg cgaaggactg tacacgcaca tgaaagccct tcgccccgat gaagaccgtc
     4381 tttctccgtt gcactcggtc tatgttgacc agtgggactg ggaacgcgta atgggcgacg
     4441 gtgagcgtca attctcgact ctgaaaagca cggtagaggc gatctgggcg ggaattaaag
     4501 caaccgaagc tgcggttagc gaagagtttg gcctggcacc gttcctgccg gatcagatcc
     4561 acttcgtaca cagccaggag ttactgtctc gttatccgga tctcgatgcc aaagggcgtg
     4621 agcgggcgat agcgaaagat cttggcgcgg tattccttgt cgggattggc ggcaagctga
     4681 gcgatggtca tcgccacgac gtgcgcgcac cggattatga tgactggagc accccgtcag
     4741 agctgggtca tgcgggtctg aacggcgata ttctggtgtg gaaccctgta ctggaagatg
     4801 cgtttgagct ttcttccatg gggatccgcg tggatgccga cacgttgaag catcagttgg
     4861 cgctgaccgg tgatgaagat cgtctggagc tggagtggca tcaggcgctg ctgcgcggtg
     4921 aaatgccgca gaccatcggc ggcggtatcg gccagtctcg tttgaccatg ctgctgctgc
     4981 aactgccgca tatcggccag gttcagtgtg gtgtatggcc agctgctgtt cgcgagaacg
     5041 tcccttctct gctgtaataa tttatcgccg ccagcgtctg agcaggcggc ttcgcatccc
     5101 ggtatcaaag cgccagatat gatcgaaaat gcgcatgatg ccgggtttgc cgtgtgccga
     5161 catcgccacg gcatgaaagc gatgctgatg tacccgctgc agctctttca ctttactcgt
     5221 cacgtcgtca ggcaaccgct gagcgataaa atcagaaatc accaccgcgt cggcatcaaa
     5281 ccattccctg ctttgcaagc gttccataat ggcgcgaaaa caactggcaa gatcggtgcc
     5341 gccacgaaac tgctggctta aaaaacggat tgcttgttcg atgccttgtg ggcctgaaag
     5401 ctcataacgg acgatctcgg tggaaaatag cataatatag cagcgccggt tttcagcgag
     5461 agcaatgcgc atcaaggcca ggcagaacgc tttcgcacac tgttcattaa agccgcccat
     5521 tgagccggaa gtatccacac agacaataaa cggcccgcgc ggctgttcat cgtaatcttt
     5581 atgtaccacc ggacgttcga tcactttttc acgccacgac tcaccgtgca ggcgataggt
     5641 gagcaactgt ttttccacca gccgacggta aaactcatac tccagttccg ttatccctag
     5701 tgtcgccagt tctggcggca ggagacgtaa aatatcatcg ctttgttgca gaccatcaac
     5761 ctgctcagga acagttgccg gttcgcgcac catggtgcgg aaggcttcca tctgcgcatc
     5821 gttacgcggt attgatttgg cttcccggga acgccccagc tgttctgcca ggcgtttcag
     5881 ttccggctgt tcgttaagaa attcaccgta tttcacaatc aactgatagt cgccacgttt
     5941 aagctgaccg gcgctcatat cccacagacg accagctgcg gtattgttat ctgcgagaat
     6001 cggttcaagt tgtccgctca gcgtcatgcg ttcctgaact tcacttaaca gttgttcgcg
     6061 ttcttcttct aatagctgtt gatttaacgt cgttgcttgc acgatcagac ttaaacgcca
     6121 acgctggagg aaaagcgtat gtaacgctga agtgatcgtg ctgttagcat caaccaactg
     6181 tcgggcttgt tctgcccatg gggaattcag acgatgcagt aagtccagga tctgtggtag
     6241 ctgcacaata aactgcggcg tggagaggag ctggctttgc tgatagcaca tcacctcttc
     6301 ggtgagttcc ggcgggactc gggcatcttt cagccgactg cgcagcgcct cacgccagcg
     6361 gggaacatca tcagtgattg cagccttcag tcgtgggaat ttttcaaaga agactgccag
     6421 ctgcggtgag gccagcagcg cgatgatcat ctcttcgatc aatccctctt cgctgacggc
     6481 cagcatcaca ttaagcgtat ccagcgttag cattgttgtg cctggcgaat ctgtgcgccg
     6541 acatcctgta ggctggcttc aatgcgacct aaccagtcgc cagggataaa cagacatttt
     6601 tgctgttcgc tgaataaagc gtgttgcttg cgccagtcac tttcaagctc ttccagttgt
     6661 tgttttattt cacctggcag accttcagcc gatgaaccgg gaagtgccag cgtactgcct
     6721 tgtaaactta catcgcgtac aacaagatgt tgggcgctat ccacttccag attcaatttc
     6781 tgggcaaagc cgataccgtt cagtttgccg cgaatttcac cacctttact cagccactgt
     6841 tccagcgcgt tacgctcaaa ggagatatga accacttcca tatcatgcaa ttttaacggt
     6901 ttttgcagca gcagagtcag agtggaagca gtaacgttaa caggaagttg atactgctga
     6961 cgacggctga aaatgccgcc cagacgaatt accgttaagg ctgttttatc gctttgttgc
     7021 tgctggagtt gcaggtgacg ttgcacaatc gcgccaaggc gagtcaacat cccttgctgt
     7081 tgccaggcgt gaccggtcat caatacatca atttgttgtt gtatcaaatt caggctttgc
     7141 gcgtcatacc acaggcaatc tttcagcaaa atgagatcta ccggggcaac agcactgcga
     7201 ccgctaaaaa aggcgctggc ctgcaataag cggatcgctt ttttccagcg acgatccgag
     7261 acataaggcg catccggtaa tttatccagt tgctggcgca gcataaaaat cagctcaaat
     7321 acatgatcgg gcagcgtaat ttcaccaatc tctttctgcc agcgttcata ttcttcatct
     7381 gtgacctgca aggcatcagg aaccggattg tcgttttcat cctgttgact ggtcagcatg
     7441 gagcggaaat tcgctttatc ctgcacttta tctaaccaca gacgaatcag catgcggtca
     7501 tataacgctt ccagactgct gtctgcttcc ggcagctcgt tggaggccgc caccagcaga
     7561 cgcatcggga ttttttctac gtgtgcaccg ttgcggaact ggcgctcgtt aatggcggtg
     7621 agcaaggtat taagaattgc cgggcccgct ttccagatct catccagaaa gacaatttct
     7681 gcttccggca ggtaaccgct ggttaaacgt tcatagcgcc cttcatcttt tagcgcctga
     7741 atagaaaggg gaccaaaaac ttcttccggc gtggagaagc gggtcatcag atattcaaac
     7801 gcgcgggcat tctgaaaggc gaattttaag cgccgggcga tcaaactttt ggcaatacct
     7861 ggcgggccaa ggaggaacac actttcacca cttaatgccg ctaataaaca caagcggatg
     7921 gcgtggctac gttcataaag ccccttttcc aacgaactgc tcaggcggga aattctttcc
     7981 gctaataaat gagggtgagc cataatgaag tggcgtcctt tcgtcaaaag ttctgcgtaa
     8041 attgcgagta tagacgtttc ttgctggtgg ctaaaatagt ctcaaagggg ggggtatttt
     8101 tctttgagcc aggttaatgt ggccgcattt aggagtacga ttttgccgtt aatcgtgcat
     8161 actgtgcgct tttttgtggg ccaagggact aagcacacat ttcatatttc aacgaaagac
     8221 tagtctatga gcactgataa taagcaatca ttgcccgcga ttaccctcgc ggcgattgga
     8281 gttgtctacg gcgatattgg taccagcccg ttatatacac ttcgtgaatg tttgtccggc
     8341 cagtttggtt ttggcgttga acgcgatgcc gtgtttggct ttttatcgct gatcttctgg
     8401 ctgctaatct ttgtggtttc cattaaatat ctcaccttcg tgatgcgggc agataacgcc
     8461 ggtgaggggg ggatcctgac gttgatgtcg cttgccgggc gtaatacgtc ggcgcgaacc
     8521 acatcaatgc tggtgattat ggggctaatc ggcggcagct ttttctatgg tgaagtcgtc
     8581 ataacacccg ctatttcggt gatgtcagcc attgaaggtc tggaaatcgt cgccccgcag
     8641 ctggatacct ggatagttcc cctctcaatt atcgttctca ctttattatt tatgattcaa
     8701 aaacatggca cggctatggt cggtaagctg tttgcgccga tcatgctgac ctggtttttg
     8761 attctggcag gactggggtt acgtagcatt attgctaacc cggaagtgct gcatgcgctg
     8821 aatccaatgt gggcggtgca tttcttcctt gaatacaaaa cggtttcttt tattgcatta
     8881 ggggcagtgg tgttgtcgat tacgggggtc gaggcgctgt atgctgatat ggggcacttt
     8941 ggtaagttcc ctattcgcct ggcgtggttc accgtcgtat taccttcctt gacccttaat
     9001 tacttcggtc agggagcgct gttgttaaag aacccggaag cgattaagaa cccgttcttc
     9061 ctgttggcac cggactgggc gctgatcccg ctgctgatca tcgccgcact ggcgacggta
     9121 attgcctcgc aggcggttat ctctggcgtc ttctcattga cgcgtcaggc ggtacgtctg
     9181 ggatatttgt cgccgatgcg cattattcac acctccgaaa tggagtcagg gcaaatctat
     9241 attccgtttg tgaactggat gctctatgtc gcggtcgtga ttgtgattgt cagctttgag
     9301 cactccagca acctggcggc ggcgtacggg attgcggtga ccggaaccat ggtgctgacg
     9361 tctattctct cgactaccgt ggcacgtcag aactggcact ggaataagta ttttgttgcg
     9421 ctgatcctga ttgctttcct ttgtgtcgat attccattgt tcaccgctaa cctcgataaa
     9481 ctgctctccg gcggctggtt gccattgagc ctcggtactg tgatgtttat cgtgatgacc
     9541 acctggaaga gcgagcgttt ccgcttgctg cggcggatgc atgaacatgg taactctctg
     9601 gaagcgatga ttgcttcgct ggagaaatca ccgcctgttc gcgtgcccgg gaccgcggtg
     9661 tatatgtcgc gtgcaatcaa cgtcattccc tttgcgctga tgcataacct taaacataac
     9721 aaggtattgc atgagcgggt gattctgtta actctgcgta ccgaagacgc gccatatgtc
     9781 cataatgtcc gtcgggtaca gattgaacaa ctgtcgccca ctttctggcg cgtggtggca
     9841 agttatggtt ggcgagaaac gccaaacgtg gaagaagttt tccaccgctg cggtctggaa
     9901 ggattaagtt gccggatgat ggaaacctcc ttctttatgt cgcatgagtc gttgatcctc
     9961 ggcaaacgcc cgtggtattt gcgtctgcgc ggcaagctgt acttgctgct gcaacgtaat
    10021 gcgctgcgtg caccagatca atttgaaatt ccgccaaaca gggttatcga actgggtact
    10081 caggtcgaaa tctaacgcca gacgcctcct ttctccataa gggggcgttt ttgttttcat
    10141 ggttaatcac catgtaaaac gtttcgaggt tgatcacatt tccgtaacgt cacgatggtt
    10201 ttcccaactc agtcaggatt aaactgcggg tcagcgaaac gtttcgctga tggagaaaaa
    10261 aatgaaaaaa ggcaccgttc ttaattctga tatttcatcg gtgatctccc gtctgggaca
    10321 taccgatacg ctggtggtgt gtgatgctgg tttacccatc cccaaaagta caacgcgtat
    10381 cgatatggca ttaacccagg gtgtaccttc ttttatgcag gtgctgggcg tcgtcacaaa
    10441 tgaaatgcag gtcgaggctg ccattatcgc ggaagagatc aaacaacata atccgcaact
    10501 ccacgaaacg ttgctcactc accttgagca gctgcaaaaa caccagggaa ataccattga
    10561 aattcgttac accacgcatg aacaattcaa acaacaaacc gcagaaagtc aggcggtaat
    10621 tcgcagcgga gaatgttctc cgtatgcgaa tatcattctc tgtgctggcg tgacgttctg
    10681 aggccgtcat ggaagcatta cttcagctta aaggcatcga taaagccttc ccgggcgtaa
    10741 aagccctctc gggcgcagcg ttaaatgtct atccgggccg cgtgatggcg ctggtgggcg
    10801 aaaacggcgc gggtaaatcc accatgatga aagtgcttac tggtatctat gctcgcgatg
    10861 ccggcacgct tttatggctg gggaaagaaa cgacatttac cgggccgaaa tcttcccagg
    10921 aagccgggat tgggattatc catcaggaac tgaacctgat cccgcagttg accattgccg
    10981 aaaacatttt cctcggtcgt gagtttgtta atcgctttgg caaaattgac tggaaaacca
    11041 tgtatgccga agcggataaa ttgctggcta aacttaacct gcgctttaaa agcgacaagc
    11101 tggtgggcga tctttccatc ggtgaccagc aaatggttga aatcgccaaa gtgctgagct
    11161 ttgagtcgaa agtcatcatt atggatgaac cgaccgatgc gctgaccgat accgaaaccg
    11221 aatccctgtt ccgcgtcata cgcgagctga aatcgcaagg ccgcggtatt gtctatatct
    11281 cccaccgcat gaaagaaatc ttcgagattt gcgatgacgt taccgttttt cgtgacgggc
    11341 aatttattgc tgagcgcgaa gtggcatcac tgaccgaaga ttcgctgatt gagatgatgg
    11401 tgggccgcaa gctagaagat caatatccac acctggacaa agcgccggga gatatccgcc
    11461 tgaaagtcga taatctctgc ggacctggcg ttaacgatgt ctcttttact ttacgcaaag
    11521 gcgaaattct tggcgtctct ggtttgatgg gcgcaggtcg taccgaactg atgaaagtgc
    11581 tctacggcgc actaccgcgc accagcggtt acgtcaccct ggatgggcat gaagtcgtta
    11641 cccgttcacc gcaggatggc ctggcaagcg gcattgtgta tatctccgaa gaccgtaaac
    11701 gtgacggttt agtgttgggc atgtcagtaa aagagaacat gtcgctgaca gcgctgcgct
    11761 acttcagccg cgctggcggc agtttgaagc atgccgatga acagcaggct gtgagtgatt
    11821 tcattcgtct gtttaatgtg aaaacaccgt cgatggaaca ggcaattggt ctgctttccg
    11881 gtggcaatca gcaaaaagtg gcgattgccc gcggtctgat gacacgcccc aaagtgttga
    11941 tccttgatga acctacccgt ggcgtagatg tcggcgcgaa aaaagagatc tatcaactga
    12001 ttaaccagtt caaagccgat ggcttgagca tcattctggt gtcatcggag atgccagaag
    12061 tattaggcat gagcgatcgc atcatcgtca tgcatgaagg gcatctcagc ggggaattta
    12121 ctcgtgagca ggccacccag gaagtgttaa tggctgccgc tgtgggcaag cttaatcgcg
    12181 tgaatcagga gtaaaaaaat gacaacccag actgtctctg gtcgccgtta tttcacgaaa
    12241 gcgtggctga tggagcagaa atcgcttatc gctctgctgg tgctgatcgc gattgtctcg
    12301 acgttaagcc cgaacttttt caccatcaat aacttattca atattctcca gcaaacctcg
    12361 gtgaacgcca ttatggcggt cgggatgacg ctggtgatcc tgacgtcggg catcgacttg
    12421 tctgtaggtt ctctgttggc gctaaccggc gcagttgctg catctatcgt cggcattgaa
    12481 gtcaatgcgc tggtggctgt cgctgccgct ctcgcgttag gtgccgcaat tggtgcggta
    12541 accggggtga ttgtagcgaa aggtcgcgtt caggcgttta tcgctacgct ggttatgatg
    12601 cttttactgc gcggcgtgac catggtttat accaacggta gcccagtgaa taccggcttt
    12661 actgagaacg ccgatctgtt tggctggttt ggtattggtc gtccgctggg cgtaccgacg
    12721 ccagtctgga tcatggggat tgtcttcctc gcggcctggt acatgctgca tcacacgcgt
    12781 ctggggcgtt acatctacgc gctgggcggc aacgaagcgg caacgcgtct ttctggtatc
    12841 aacgtcaata aaatcaaaat catcgtctat tctctttgtg gtctgctggc atcgctggcc
    12901 gggatcattg aagtggcgcg tctctcctcc gcacaaccca cggcggggac tggctatgag
    12961 ctggatgcta ttgctgcggt ggttctgggc ggtacgagtc tggcgggcgg aaaaggtcgc
    13021 attgttggga cgttgatcgg cgcattaatt cttggcttcc ttaataatgg attgaatttg
    13081 ttaggtgttt cctcctatta ccagatgatc gtcaaagcgg tggtgatttt gctggcggtg
    13141 ctggtagaca acaaaaagca gtaataacga ctacaggaca tcttgaatat gaacatgaaa
    13201 aaactggcta ccctggtttc cgctgttgcg ctaagcgcca ccgtcagtgc gaatgcgatg
    13261 gcaaaagaca ccatcgcgct ggtggtctcc acgcttaaca acccgttctt tgtatcgctg
    13321 aaagatggcg cgcagaaaga ggcggataag cttggctata acctggtggt gctggactcc
    13381 cagaacaacc cggcgaaaga gctggcgaac gtgcaggact taaccgttcg cggcaccaaa
    13441 attctgctga ttaacccgac cgactccgac gcagtaggta atgctgtgaa gatggctaac
    13501 caggcgaaca tcccggttat cactcttgac cgccaggcaa cgaaaggtga agtggtgagc
    13561 cacattgctt ctgataacgt actgggtggc aaaatcgccg gtgattacat cgcgaagaaa
    13621 gcgggtgaag gtgccaaagt tatcgagctg caaggcattg ctggtacatc cgcagcccgt
    13681 gaacgtggcg aaggcttcca gcaggccgtt gctgctcaca agtttaatgt tcttgccagc
    13741 cagccagcag attttgatcg cactaaaggt ttgaacgtaa tgcagaacct gttgaccgct
    13801 catccggatg ttcaggctgt attcgcgcag aatgatgaaa tggcgctggg cgcgctgcgc
    13861 gcactgcaaa ctgccggtaa atcggatgtg atggtcgtcg gatttgacgg tacaccggat
    13921 ggcgaaaaag cggtgaatga tggcaaacta gcagcgacta tcgctcagct acccgatcag
    13981 attggcgcga aaggcgtcga aaccgcagat aaagtgctga aaggcgagaa agttcaggct
    14041 aagtatccgg ttgatctgaa actggttgtt aagcagtagt tttaatcagg ttgtatgacc
    14101 tgatggtgac ataaatacgt catcgacaga tgaacgtgta atataaagaa aagcagggca
    14161 cgcgccaccc taacacggtg gcgcatttta tggacatccc gaatatgcaa aacgcaggca
    14221 gcctcgttgt tcttggcagc attaatgctg accacattct taatcttcaa tcttttccta
    14281 ctccaggcga aaccgtaacc ggtaaccact atcaggttgc atttggcggc aaaggcgcga
    14341 atcaggctgt ggctgctggg cgtagcggtg cgaatatcgc gtttattgcc tgtacgggtg
    14401 atgacagcat tggtgagagc gttcgccagc agctcgccac tgataacatt gatattactc
    14461 cggtcagcgt gatcaaaggc gaatcaacag gtgtggcgct gatttttgtt aatggcgaag
    14521 gtgagaatgt catcggtatt catgccggcg ctaatgctgc cctttccccg gcgctggtgg
    14581 aagcgcaacg tgagcgtatt gccaacgcgt cagcattatt aatgcagctg gaatcaccac
    14641 tcgaaagtgt gatggcagcg gcgaaaatcg cccatcaaaa taagactatc gttgcgctta
    14701 acccggctcc ggctcgcgaa cttcctgacg aactgctggc gctggtggac attattacgc
    14761 caaacgaaac ggaagcagaa aagctcaccg gtattcgtgt tgaaaatgat gaagatgcag
    14821 cgaaggcggc gcaggtactg catgaaaaag gtatccgtac tgtactgatt actttaggaa
    14881 gtcgtggtgt atgggctagc gtgaatggtg aaggtcagcg cgttcctgga ttccgggtgc
    14941 aggctgtcga taccattgct gccggagata cctttaacgg cgcgttaatc acggcgttgc
    15001 tggaagaaaa accattgcca gaggcgattc ggtttgccca tgctgccgct gcgattgccg
    15061 taacacgtaa aggcgcacaa ccttccgtac cgtggcgtga agagatcgac gcatttttag
    15121 acaggcagag gtgacgcttg gctacaatga aagatgttgc ccgcctggcg ggcgtttcta
    15181 cctcaacagt ttctcacgtt atcaataaag atcgcttcgt cagtgaagcg attaccgcca
    15241 aagttgaagc ggcgattaaa gaactcaatt acgcgccatc agccctggcg cgtagcctca
    15301 aactcaatca aacacatacc attggcatgt tgatcactgc cagtaccaat cctttctatt
    15361 cagaactggt gcgtggcgtt gaacgcagct gcttcgaacg cggttatagt ctcgtccttt
    15421 gcaataccga aggagatgaa cagcggatga atcgcaatct ggaaacgctg atgcaaaaac
    15481 gcgttgatgg cttgctgtta ctgtgcaccg aaacgcatca accttcgcgt gaaatcatgc
    15541 aacgttatcc gacagtgcct actgtgatga tggactgggc tccgttcgat ggcgacagcg
    15601 atcttattca ggataactcg ttgctgggcg gagacttagc aacgcaatat ctgatcgata
    15661 aaggtcatac ccgtatcgcc tgtattaccg gtccgctgga taaaactccg gcgcgcctgc
    15721 ggttggaagg ttatcgggcg gcgatgaaac gtgcgggtct caacattcct gatggctatg
    15781 aagtcactgg tgattttgaa tttaacggcg ggtttgacgc catgcgccaa ctgttatcac
    15841 atccgctgcg tcctcaggcc gtctttaccg gaaatgacgc tatggctgtt ggcgtttacc
    15901 aggcgttata tcaggcagag ttacaggttc cgcaggatat cgcggtgatt ggctatgacg
    15961 atatcgaact ggcaagcttt atgacgccac cattaaccac tatccaccaa ccgaaagatg
    16021 aactggggga gctggcgatt gatgtactca tccatcggat aacccagccg acccttcagc
    16081 aacaacgatt acaacttact ccgattctga tggaacgcgg ttcggcttag atttacgctg
    16141 tcttttgatc aaattattac catcggttgt tttcagaagc atgaacattg ctgctgaagc
    16201 aacagtaata atgcccatcg tgataaacgt atagtggaat tgttcgacag tcgttgtgcc
    16261 ttccatccct tcataaacgc gaaggacggc cgcacttaca gcaacgccta aactaatcga
    16321 cagttgctgc gtgaccgcca gaacactgtt accgctgctg gcgttgtcat cggtcagatc
    16381 ggcaagtgtg atggtattca tcgcggtaaa ttgcgtcgac atagccatcc ctaatataaa
    16441 caacggcaag atcagcatcc atatcgccat tgccggtgat tgcaaagaga actgagcgat
    16501 cattagccca ataatcaccg tgatccccac taacgtatgg cgatagccca gacgacgtaa
    16561 gacttgggta accatcgatt ttgcaataat ggaacctaac gctgtcggtg ccatcataca
    16621 gccggcaata aacgcctgat aaccaaatcc tacctgtaac atcaatggca taaggaacgg
    16681 tacacaaccg gtccccagac gggttgcaat attgcctacg ataccgatcg agaaagtgcg
    16741 ggttttaaat aaatctaatg aaattaatgg gtttggcgtg tgtcgcgcat ggagaatata
    16801 gagaagcagt aacccgatgc tggtgacaat taccgtcaag gcaatccagc tggcgacaat
    16861 cttttccccg aatagctcta ttccgcttga gaagagaaca aggctgaggc caaacagcaa
    16921 aaagccagtg atatcgaatc tgcgtcgtgc ggtggtgaaa ttgggcatat gtttgcgcgc
    16981 gtaaagaagg cccgcaatac ctatggggat attgattaaa aatatccagt gccaggttgc
    17041 ccaggtgacc agcacgccgc caagaacggg gcctaaaatt ggccccacca gacccggcat
    17101 ggcgacaaaa ttcaatactg gaagaagttc attacgagga taagcgcgca gtaaggccag
    17161 ccgagcaaca ggcatcatca ttgcgccgcc tatcccctga ataacccgga agacaaccag
    17221 ctgtggtagc gaattagaaa gtgcgcaggc cagagaaccc aatgtgaaca gactcacggc
    17281 aagggtaaaa atgcgacgcg taccgaagcg atcggctagc catccgctta ccggaataag
    17341 catcgccacc gtcagcgtat aactgatgat ggctgattgc atcgcgagag gagaacgatt
    17401 aaggctatga gcgattgcgg gtaaggcggt attcagaata gtggcatcaa gtgcctgcat
    17461 gaagaaggcc atcgccgcga tccacggcaa acccgccata ctgcgcttct ttttatcgct
    17521 cattcaatgt cctgttatcg ggttgtcact tatcaggtga gcgtagcagc gcctgacaag
    17581 ctttaaatgc cgcgtcgcca tcgctttgga caatcgcatc gacaatcgcc tggtgcagat
    17641 ccagctttat cactgtgtcg ctggtaattg acgtgaagta agtgtgataa accgaatgga
    17701 atagcgaggc gaatgaggtc aaaaacggat tggcgctcat ttcatagata tgctcatgcc
    17761 aggccatatc gacttcgatc cagcgttcac ggcgaaagtt ctcttttaat gccgccattt
    17821 cggccattaa cgtattgaga tgcgccttct gttccgcggt gccaaccgtt gcagccagta
    17881 ggcaagcttg cggctccaga cagatacgca taaccagaaa gtgatcgatg acctgatgaa
    17941 agttctcttc tgtcatccac caggtaagca attcctgatc aagaaaattc cagtttgatt
    18001 gtggcatgac ccgggtacca attcgcggtc gcggtaaaac catccctttt gccgttaacg
    18061 ttttgaccgc ttcgcgtacc gctgtacgac tcactccaaa ttgctcgccc agctcaatct
    18121 caccaggcaa aatggtgccg ggttcatatt caccttttaa gatgcgttgc gccagcttct
    18181 cagccagaac atacgaaagg tttttctgtg cagctaactg ttgtgcgctt aaaggcatta
    18241 ctaatcttcc tttttctttt tattcctcct tagtatgcca ccaggaagtg tgattacggt
    18301 tgcaaaaacg gcaaattgct tgttttatgg cacattaacg ggggttttgc t
//
LOCUS       DAPHDW010000087        18096 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.087, whole genome shotgun sequence.
ACCESSION   DAPHDW010000087 DAPHDW010000000
VERSION     DAPHDW010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 18096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18096
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.087"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(21..668)
                     /locus_tag="RH057_002955"
     CDS             complement(21..668)
                     /locus_tag="RH057_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV3129294.1"
                     /translation="MLLLIWWLTAAGVWATAGCAWIFQHLHWVTSQKSPGAFVFALAA
                     LIIGGLWLNNALKRLNVSGACLVYLLIILLVGFLFASLYTFSSIATVMGITGGMFAAM
                     ALICSCSNRVIPPVRQLYSYIFCGLSIAFVVNLILTSSFSVWLASILTVFIWGITAAC
                     EATTLEDLIRVADTSEISGSLRCIVPGAITLYFSILSVLFRITVTVLEFINGLVW"
     gene            complement(870..1574)
                     /locus_tag="RH057_002956"
     CDS             complement(870..1574)
                     /locus_tag="RH057_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HDV3129295.1"
                     /translation="MDRFPRSDSIVQPRAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            complement(1711..2163)
                     /gene="moaE"
                     /locus_tag="RH057_002957"
     CDS             complement(1711..2163)
                     /gene="moaE"
                     /locus_tag="RH057_002957"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HDV3129296.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDQQAAKRW"
     gene            complement(2165..2410)
                     /gene="moaD"
                     /locus_tag="RH057_002958"
     CDS             complement(2165..2410)
                     /gene="moaD"
                     /locus_tag="RH057_002958"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HDV3129297.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADFPTVEALRQHMSAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            complement(2403..2888)
                     /gene="moaC"
                     /locus_tag="RH057_002959"
     CDS             complement(2403..2888)
                     /gene="moaC"
                     /locus_tag="RH057_002959"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HDV3129298.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            complement(2891..3403)
                     /gene="moaB"
                     /locus_tag="RH057_002960"
     CDS             complement(2891..3403)
                     /gene="moaB"
                     /locus_tag="RH057_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HDV3129299.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDKAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            complement(3425..4414)
                     /gene="moaA"
                     /locus_tag="RH057_002961"
     CDS             complement(3425..4414)
                     /gene="moaA"
                     /locus_tag="RH057_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HDV3129300.1"
                     /translation="MASQLTDAFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEDDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     regulatory      complement(4402..4546)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            4811..5719
                     /gene="yvcK"
                     /locus_tag="RH057_002962"
     CDS             4811..5719
                     /gene="yvcK"
                     /locus_tag="RH057_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HDV3129301.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPSVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     gene            complement(5911..7932)
                     /gene="uvrB"
                     /locus_tag="RH057_002963"
     CDS             complement(5911..7932)
                     /gene="uvrB"
                     /locus_tag="RH057_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HDV3129302.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVVVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMNELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDKITPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMSPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8511..9188)
                     /gene="bioD"
                     /locus_tag="RH057_002964"
     CDS             complement(8511..9188)
                     /gene="bioD"
                     /locus_tag="RH057_002964"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HDV3129303.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAEPTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            complement(9181..9936)
                     /gene="bioC"
                     /locus_tag="RH057_002965"
     CDS             complement(9181..9936)
                     /gene="bioC"
                     /locus_tag="RH057_002965"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HDV3129304.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADVLLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLVQARQKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQAWQAVDERP
                     HANRFLPPDEIEQSLNGVHYQHHIQPITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            complement(9923..11077)
                     /gene="bioF"
                     /locus_tag="RH057_002966"
     CDS             complement(9923..11077)
                     /gene="bioF"
                     /locus_tag="RH057_002966"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HDV3129305.1"
                     /translation="MSWQEKINAALAARRTTDALRRRYPVTQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPHIIRAWQQGAEQFGVGSGGSGHVSGYSVAHQALEEELAEWLGYSRA
                     LLFISGFAANQAVITAMMAKEDRIVADRLSHASLLEAASLSPAQLRRFAHNDVTHLAR
                     LLAASCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRSEEGDARREKLAALITRFRAGVQGSPFTLADSRSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEMLHGNG"
     gene            complement(11074..12114)
                     /gene="bioB"
                     /locus_tag="RH057_002967"
     CDS             complement(11074..12114)
                     /gene="bioB"
                     /locus_tag="RH057_002967"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HDV3129306.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFDPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLRQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLEQALMT
                     PDTDEYYNAAAL"
     gene            12201..13490
                     /gene="bioA"
                     /locus_tag="RH057_002968"
     CDS             12201..13490
                     /gene="bioA"
                     /locus_tag="RH057_002968"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HDV3129307.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKSQIDAMSHVMFGGITHTPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAILESGDWQQ
                     QVADIEVQLREQLAPARNAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            13549..14025
                     /locus_tag="RH057_002969"
     CDS             13549..14025
                     /locus_tag="RH057_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HDV3129308.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDIERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            14177..15460
                     /locus_tag="RH057_002970"
     CDS             14177..15460
                     /locus_tag="RH057_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HDV3129309.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEAQNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     AFGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEAKK"
     gene            complement(15694..17955)
                     /locus_tag="RH057_002971"
     CDS             complement(15694..17955)
                     /locus_tag="RH057_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HDV3129310.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAEFTLSRRDPGYVGRSKATAELENQRLAGNVSELTEVFARIKQIAGQEHIDPL
                     QTEIGSMVYAVKPGDGSAREQAASCQRVIGGLANIAEEYATKRYRSNVINWGMLPLQM
                     AEVPTFEVGDYIYIPGIKAALDNPGTTFKGYVIHEDAPVTEITLYMESLTAEEREIIK
                     AGSLINFNKNRQM"
BASE COUNT         4264 a         4894 c         4605 g         4333 t
ORIGIN      
        1 ataaaactcg tcaattcgca ctaccacact aaaccattaa taaattcaag aactgttaca
       61 gtaattctaa agaggacgct taaaatacta aaatagagtg ttattgcccc aggaacgata
      121 cagcgaagag aacctgaaat ttcagaggtg tcggcaacac ggatcaaatc ttccagtgtt
      181 gttgcttcac aggcagccgt aatgccccag ataaagacag tcaaaatact cgctaaccac
      241 acggaaaaac tgctggtaag gatcaggtta acaacaaaag caatactcaa accacagaaa
      301 atatatgaat ataactgtct gactggtggg ataaccctat tactacaaga gcaaatcagc
      361 gccattgcgg caaacatccc tcccgtaatt cccattaccg tcgctatgct cgaaaaggta
      421 taaaggctgg cgaagagaaa gccaacaaga aggattatta gcagatagac cagacatgcg
      481 cctgatacat tcagtctttt cagggcgttg tttaaccaga gaccacctat tatcagcgca
      541 gccaaagcga agacaaacgc acctggagac ttttgtgacg taacccaatg caaatgctga
      601 aatatccagg cacagcctgc cgtagcccat actccagctg ctgttaacca ccaaatgaga
      661 agcagcattt tgctgagcga agcacttttt tgctgtaatt gagtagcagt ggattgtctg
      721 ttcaaatgcg ttatctgtcc ctgaaattac gtagtggaca tagtctggca tttgccgcga
      781 ttcagttctc atgtaaatga gcggaatagt ctttataaaa tccgaaaaaa atcccggctc
      841 caggccggga tcagcaggaa gaagagaatt taacggcggt tgccgaagat ccgcaacaac
      901 atcaggaaca ggttgatgaa gtccagatac aaggttaacg cgccaagaat ggaatatttg
      961 cgcaggttcg acgtgtcgcg ggtatcaatc tgctcaccca tatttttcag tttctgcgtg
     1021 tcatacgccg tcaatccgac aaagacaatc acgccgatgt aggtaactgc ccacatcaat
     1081 gcttcgcttt tcaaccagaa gttgaccagc gatgccagca caatgccgat taacgccata
     1141 aacagcatat tgccaaagcc acttaaatcg cgcttcgtgg tgtaaccgta caggctcatt
     1201 gcgccgaaca tcccggcagt aacgacgaaa gtactggcga tagaagcagc ggtatagaca
     1261 atgaatatac tggaaagcgt aagacccgtc agcgccgaat aaagcataaa gagcatcgtc
     1321 gttacacctg cgctcagctt ttgaatcatc gctgataaca caataaccaa tgctaattgc
     1381 gcgatgatca gaccgattaa aaagacacgg ttagtgaaca acagctccat cacggccgcg
     1441 gaattagccg cataccaggc aacaaatgcg gtcagcaaca agccaacggt catccagcca
     1501 tagacttgcg ccatataagt ttgcaagcca gcccggggtt gtacgattga atcagaacgt
     1561 gggaatctgt ccatgacgat ctcctgaaga tataaggaat atcttaagga tactgcaaaa
     1621 tgatgaggct gtgcatcgac gcagcgtaaa cgtatgtact gagcggtgaa attgccggac
     1681 gcagcggtgc cttatccggc taacaaaaaa ctaccagcgt tttgccgcct gctgatcgct
     1741 ctcccgggct tcaacccagc ggtcgccttc cggcgtggct tcgcgcttcc agaacggtgc
     1801 gcgggttttg agataatcca taataaactg cccggcttca aacgcactgc tgcgatgcgc
     1861 actggtgaca ccgacaaaaa cgatttcatc gcccggccat aattccccga tgcggtgaat
     1921 cacagtgacg cgccccagcg gccagcggtt acgcgcttca tcaacaattt ctgccagtgc
     1981 tttttcagtc atccccggat agtgttcgag ggttaatgct ttgacgctgt cgcccaggtt
     2041 atggttgcgc accttaccag taaaggtgac taccgcaccg tcttcgtcac gctccgccag
     2101 ccacgggtac tcttctccta cgctgaacgg ctgcggacca acaacaattt tggtttctgc
     2161 catcttaacc tccggttacc ggcgggaaga aagctacttc gtcgccgtca gtcagcggat
     2221 ggtcaaaact caccagcgtc tggttgacgg cagccagtaa tttgccatct tccagcgcca
     2281 gcgcccagcg atcgctctgc gcagacatgt gctggcgtaa cgcttcaaca gttgggaaat
     2341 ccgcagccac ttcggttgca tctgttccca ccaactcgcg cacctgggcg aaaaaaagaa
     2401 ctttaatcat ccgcttccac cttaaagtca cccgacttgc cgccgctctt cgccagcaaa
     2461 cgtaccggac caatcaccat atctttttgc accgctttgc acatgtcata aatggtcagc
     2521 gccgccacgg aggccgcggt taatgcttcc atttcgacac cggttttccc ggtcaggcgg
     2581 cataaggttt ctatacgcac ccgattgtgc tccggctcgg cctgtaaatt gacttcaact
     2641 ttgctgagca tcagcggatg acagagcggg atcagatccc aggtgcgttt tgccgcctga
     2701 ataccggcaa tacgcgcagt ggcaaatacg tcgcctttgt ggtggcgacc atcaataatc
     2761 atcgccagcg tctcgctgcg catggtgaca aaggcttccg cccgcgcttc acgcacggtt
     2821 tccgctttgg cggagacatc caccatgtgc gcttcgccag cggcgttgat atgggtcagt
     2881 tgcgacatac ttatttcttc aaatgtggat ggaaattaca cggacgcgta cgggcatcca
     2941 gctgcggcgc gatgatattt tcccatgcgg tacggcacgc tttggtcgaa cccggcatgg
     3001 cgaaaatcag cgttttgttg gcgacgcccg ctaccgcacg agattgcaac gtggaagtgc
     3061 caatctcttc aaaagacaac atccggaaca cttcaccaaa accttcaact tcacggtcga
     3121 acaacggcag caatgcttcg ggagcctgat caccttccgt caggccagta ccacccgtaa
     3181 tcaataccac ttgtacatcg tcgctggcga tccacgcaga tacctgagcg cgaatagcgt
     3241 agcggttttc tttcacaatg gctttatcga caacgtgatg gcccgcttct tgcgccgaat
     3301 cgcgcagata gtgaccggag gtatcgtctt cttcaccgcg acgattagaa accgtaagaa
     3361 tagcaatacg ggtcgggata aattcagtgc ttacctgact catctgatct ctccttttga
     3421 cgttttagcc gccaatgtac gataagtttt gcgtaatacc ggtgttgttt tgatgcagga
     3481 aatgggtctg tttcttctcc cgcagcgccg ctgaaatacg cgcttccagc gcctgttgct
     3541 gggtatcgtc ttccagcaga tcgcgcaggt taacgccgcc ttcaccaaac aggcagagat
     3601 ggagtttacc aatggaggaa acgcgcaggc ggttgcaagt ggcgcagaag tctttttcat
     3661 acggcatgat aaggccaatc tctccggcgt aatctggatg gcaaaagact tgcgcgggac
     3721 cgtcgctgcg ttgacgtaat tggtggatcc agccgcgacg cagtagctcg tcacgcagaa
     3781 cctgaccaga gatgtgatgc ttacggaaga gctcgctgcc ctcgcccgtt tccatcagtt
     3841 cgatgaaacg cagctggata gggcgatgct ggatccagtt cagaaaggtg tcgagctggt
     3901 gatgattaac atcacgcatc agcacggtat tgactttgac cttctcaaaa ccggcctcaa
     3961 atgcagcatc aatccccgcc atgacctggt tgaatttatc ctgcccggta atagcgtgaa
     4021 actggcgggc gtccagactg tcgacactga cgttaatgcc agtaagtccc gcatcgcgcc
     4081 agttcgccac atcgcgttcc agacggtaac cattggtggt gaccgcaatc tggcggatag
     4141 cgtcgttttc ccgcacagcg gcgatgatat cggtaaagtc gcggcgtaaa gacggctcac
     4201 cgcctgtcag gcgcactttt tcggtgccca gactggcgaa ggcgcgcgta acccggcgaa
     4261 tttcatcgac ggtaagaaag cctttattgg tgacgccgct cggtttgtag ccatccggca
     4321 ggcagtaggt gcaacgaaag ttacacacat cggtaatcga caggcgcaag tagtaaaact
     4381 tacgcgcaaa tgcatcagtc agttgtgaag ccatgtacac ctttccagat acgggaggcg
     4441 aagtcatttc ttccttcgcc ctggtggcaa tctttacgag cattgtcacg gccaaagcac
     4501 cgtatcagtt gacccaggtg cagaggctag agtgtttagt ggttatgccg atactagcgt
     4561 gtaaatgaga tttttaccat ccacactttc gctatgtaat catgtatata gcgtcatgat
     4621 cgtgctattt cgctacagaa taagggaaaa tggcggtttt actttgatat acatcatcag
     4681 ggaggcatag cgtgcatcgt cctttagagg tagaagaaaa tagtttgtat cctcaatatt
     4741 ggcaggttaa ttgctgtttc cccgcaattt gcgctactgt agcgcggata atctgactcc
     4801 aggaaactat atgcgcaatc gtacgctggc tgatcttgat cgtgtcgttg ctctcggcgg
     4861 agggcatgga ctgggacgcg ttctctcatc actttcgtct ttgggttctc gtttaacggg
     4921 tatcgtcacc accaccgata atggtggctc gacggggcgt attcgccgtt cagaaggcgg
     4981 cattgcctgg ggcgatatgc gcaactgcct caaccagctg ataacggaac cgagcgtcgc
     5041 ctccgcgatg tttgaatacc gttttggtgg caatggcgaa ctttccggtc ataatctcgg
     5101 aaacttgatg ttaaaggcgc tggatcacct tagcgtgcgg cctctggaag ccatcaattt
     5161 aattcgtaat ctgctgaaag tggatacgca tttgattcca atgtcagagc atcccgtcga
     5221 tctgatggcg attgacgatc aggggcatga agtttacggc gaggtcaata tcgaccagtt
     5281 aactacgccg attcaagagt tattgttaac gcctaatgta cccgcaacgc gtgaggcggt
     5341 tcacgctatc aatgaagcgg atctcatcat tattgggcct ggcagttttt ataccagcct
     5401 gatgccaatt ctgctgctga aggaaatcgc ccaggcatta cgccgcacgc cagcgccgat
     5461 ggtttatatc ggcaatctgg ggcgtgagtt gagtttacct gcggctaatt taaagctgga
     5521 aagcaagctg gcaattatgg agcagtatgt tggtaaaaag gtcattgatg cggtcatcgt
     5581 cgggccaaaa gtggatgtct cggcggtgaa agagcggatt gtgattcagg aggtactgga
     5641 ggccagcgat attccgtatc gtcatgaccg ccagttgtta cataacgcgc tggaaaaggc
     5701 gttacaggct ttaggttaac ggaaatccga agaaaaattc cggcttccta ttgaagacaa
     5761 agtgcgcgtt gtttatgccg gatgcggcgt gaacgcctta tccggcctac aaaccgcgct
     5821 aattcaatgt attgcagaga tcatgtaggc ctgataagcg tagcacatca ggctgttttc
     5881 cgtttgtcat cagtcttctt cgctatcctg ttacgatgcg gcaataaaca actcacgcaa
     5941 ctgatgcaac tggtcacgaa tttgtgccgc ttcttcgaac tccagattct gcgcgtgttg
     6001 catcatcagc ccttccagtt catggatttt ctgctgcaac gctttcggcg acatatccat
     6061 cggcacatta tccggctcaa caatcgggcg cgattttcct ctgcccttcg ctttggtttt
     6121 ggcaatgttc tgccccagcg ccaggatatc gaccactttc ttgttcaagc cttgcggcgt
     6181 aatgccgtgt tcctcgttgt acttctgctg tttctcacgg cgacgttcgg tttcgccaat
     6241 cgctttcgcc attgatgggg tgatcttatc gccgtagaga atcgctttac cgttaacgtt
     6301 acgtgccgca cgaccaatgg tctggatcaa cgaacgttcg gaacgcagga agccttcttt
     6361 gtcagcgtcg aggatcgcca ccagcgacac ttccggcata tccagacctt cgcgcagtaa
     6421 gttgatccct accagcacgt cgaactcacc cagacgcaag tcgcggataa tctccatacg
     6481 ttcgacggtg tcgatatctg agtgaagata acgcacgcgc tcgccatgtt cttcgagata
     6541 ttcggtaaga tcttccgcca tccgcttggt cagtgtggtg accagtacgc gttcgttaat
     6601 tgccgctcgc tgacgaatct ccgaaagaag atcatcaacc tgtgtcgcca ccggccgcac
     6661 ttcgataatc gggtcaagca atccggttgg acgcaccacc tgatccacca catcgccgcc
     6721 ggatttttcc agctcgtaat tacccggcgt cgccgaaaca tagatggttt gcggcgctaa
     6781 tgcttcgaac tcttcaaact taagcggacg gttatccagc gctgatggca ggcggaagcc
     6841 gtactccacc agtgtctctt tacgcgcccg gtcaccgcga tacatgccgc caatttgtgg
     6901 aatggtgaca tgagattcat cgaccaccag cagcccatcg gcagggaggt aatcaaacag
     6961 cgtcggcggt ggctcacccg gtccacgacc ggagaggaag cgcgagtagt tttcaatccc
     7021 cgaacagtag cccagctcgt tcatcatctc cagatcaaac tgggtacgct gggtcagccg
     7081 ctgctcttcc agcagtttgt tgttttccaa cagcactttg cgtctggcgg ccagctcttc
     7141 tttgatctcc tccatcgcct gtacgatgcg ctcgcgcggt gtgacgtagt gcgttttcgg
     7201 gtagatggta aaacgtggaa tagtggaaac aatctgcccg gtcagcgggt caaataacga
     7261 caatcgttcc acttcctcgt caaacagttc cacgcgaagt gcaatgtcat ccgattctgc
     7321 cgggaagata tctatcacct cgccacgaac gcggaaagta ccacgctgga atgcttgatc
     7381 attacgagcg tattgcagct ccgccagtcg gcgcagaatc gcgcgctgat cgataatcat
     7441 accgaccgtg agatggagca tcatcttgag atataaatca ggatcgccca gaccataaat
     7501 cgcggaaaca gacgccacca caaccacatc acgccgctcc agcatcgctt tggtggcgga
     7561 caaacgcatc tgctcaatat gttcgttaac cgaggcatct ttctcaatga aagtgtcgga
     7621 actcggtaca taggcttccg gctgatagta gtcgtagtag gaaacgaaat attccaccgc
     7681 gttttccggg aagaactctt tcatttcgcc atacagctgg gccgccagcg ttttgttggg
     7741 cgcaagtacc atggttgggc gctgaaggtc agcaatgaca ttggcaatgg tgaatgtttt
     7801 cccggagcca gtcacgccaa gtaacgtctg gtgcgccagg ccatcttcca gcccctcttc
     7861 gagacgtcga atcgcctctg gctgatcgcc agaaggttta aaagcggaat tcagtttgaa
     7921 cggtttactc atgagtcgct acctgaagga gttgggcggg caggtatgta attttactcg
     7981 tcgtacttaa ttatgccaac aaattatact ggataaaaaa acagttcatc accataatat
     8041 ttctgttaca gcgtaaactc cgctgtcaat catgagcaaa atttactctg tggcgagata
     8101 aaactccggc cttaccgggt tatccccaaa gcaacggctt ttttaacatt tgtcaagatg
     8161 agtgatgaca gttttatggc aagagatgcc tgttcagtga ctgccattgc atttatctaa
     8221 ccagttaaaa aataaaagat atttctttga gccgtcttta acgccaattc gcgtgcaagc
     8281 cgcgtattct ctcgcttgcc tcgtgttttc taactctaat acacatggtt atccacagga
     8341 atagtggata actgtctcca gcccctatcc cccgccgcct gggcaattcc cacattcgcc
     8401 gcatgaggcg gaagtaaaat ttttttcgtg attattttag aatttaattg gttaaattgc
     8461 agtcaatcga agacgcgatc tcgctcgcaa tttaaccaaa tacaggatag ctacaacaag
     8521 gcaaggttta tgtactttcc ggttgccgca ttttctggat tttctgcaag ccaggggatc
     8581 tctcccagca gcggcgcggg aatcatgcgg gtgagcgtgg tcatatattc agcgtgacgt
     8641 tttcccggag gcgtaacatc gttcgccacc caacccgcca gagtcagtcc ggcgtgttgt
     8701 attgcctgcg cagtcaacat cgcgtgatta atacagccga gtttcacacc aactaccagt
     8761 atcaccggca gttgttcctg tgttacccaa tctgcaaaag tgaaagtgtc agaaagcggc
     8821 gtaaaccagc cgccagcacc ttccactaac acccagtcag cctgttgttc aagcgcgcgt
     8881 aatccggcgc tcattaccaa tgattctatc ggcctgccct cttgcgcgct gatgatgtgc
     8941 ggcgaggtgg gttctgcgaa ggtgtaagga tttaccgttg cgtaatccag ctgcaggctg
     9001 ctgttgtgct gtaacgccag cgcgtcacta ttacgtaagc cttccggggt cttttcgctg
     9061 ccagaggcga ccggtttata acctgccgtc cgataaccta ctgccttcgc ggcttgtaaa
     9121 agtgcacaac tggcgacggt tttccccact tcggtatccg ttccggtgac aaaataacgt
     9181 ttactcacga gcaatcactc ccaaaaaaag atgatacgtc agaggatatc gcccctgttg
     9241 ttgcggccag gccagttgca atcgctgcaa ttgcgaacgc gttaatattc gcgggtcgcg
     9301 cccttcatga agatgcgtag caccgatgcc tttcagcgaa cgcatggcac tgagcgcatc
     9361 atcaaaccat agtgtgatgg gctgaatatg atgttgataa tgcacgccgt tcagcgactg
     9421 ttcgatttca tctggcggta aaaagcgatt agcatgcgga cgctcgtcca ctgcctgcca
     9481 cgcctgatgc agttcgggta acgatccctg caccagcgtg gtaaacgcga ccacgcctcc
     9541 ggggcgcacc acccgataca gctcgcggag tgccgtggat aaattaccgc accactgcac
     9601 cgcgagattg ctccatgcaa gatcgaacgt cgcagtcgct aacggcaggg attcgatatc
     9661 tcccgccaga taatggtctg cggcatcctt ctggcgtgcc tgaacaagca ttggcggcga
     9721 gagatctaac gccgtcacct gcgcgtgacg ctctcgccag tggcggctca tccagcctgg
     9781 cccacacccc gcgtccagta cgtgggtgta tttacgctgt gggagcattg ccagtaagac
     9841 gtcagcactc tggcgctgta gatctgcgtg ttgctcatag tgcgcggctg cccgaccaaa
     9901 tgccgctgca atggcttgtt tattaaccgt tgccatgcag catctccagc aaacggtcaa
     9961 tatcctgcat ttcatgggcg gcggttaagg tcaggcgtag ccgcgcggtg ccagcgggta
    10021 cggttggcgg gcgaatcgcc gtcacccagc aaccttgctg gcgcagtttt tctgccagct
    10081 gtaacgcacg gctgttatca ccgacgatca atggctggat ggcgctacgt gaatccgcaa
    10141 gcgtaaacgg cgaaccctgc actccggcac gaaaacgcgt aatgagtgcc gccagttttt
    10201 cgcgccgagc atccccctct tcactgcgaa tgaccgccag cgacgcacgt aatgcctgcg
    10261 cctgagcggg cggcatactg gtgctgtaga taagatggcg ggcgaattgc agcagataat
    10321 ccgccaccgt actggagcaa agcaccgctg ccccgctgac gccaaatcct ttgccaaaag
    10381 tcactaccag caattctggt tttacctttt gcagccagca gctgccgcgc ccctgctccc
    10441 cgataacgcc cgtgccgtgg gcatcatcga ccatcaacca gccattgtgc tgttgcgtta
    10501 cctgctggat ttccgccagt ggcgcactat cgccgtccat gctgaacacg ccttctgtca
    10561 ccaccagttg ctgccccgga caggaggcag caagcaggcg cgccagatga gtaacatcgt
    10621 tatgagcaaa acggcgaagc tgcgccgggc ttaaactggc agcctccagc aaggaggcgt
    10681 ggctaagccg gtcggcaaca atacggtcct cttttgccat catcgcggtg atgaccgcct
    10741 gattagcggc aaaaccggag ataaacagca atgcccgcga atagcccagc cactcggcca
    10801 gttcttcttc cagcgcctga tgcgccacgc tataaccgct gacatgcccg gagccgccgc
    10861 taccgacgcc aaattgttcc gccccctgct gccaggcgcg gatgatgtgc ggatgatggc
    10921 ttaagcctaa gtaatcgtta ctggaaaagt tcagatactg gcgatcgtcc gccaccagcc
    10981 agcgtccggc tccttgtgtt accggataac ggcgacgcag ggcatcggta gtacgccgcg
    11041 cagcgagcgc cgcgttgatt ttctcctgcc agctcatagt gctgccgcgt tgtaatattc
    11101 gtcagtgtcc ggggtcatca gcgcctgctc cagacgctgc tgttgttcgt tatcccctgc
    11161 cagcacggca gtttgctgcg gatttagccc cagtttgcgg aacagttgca ggtctttatc
    11221 ttcttccgga ttcggcgtgg tcagcagttt gcaaccgtag aaaatcgagt ttgcgcctgc
    11281 cataaagcac atcgcctgag tctgttcgtt catctgctcg cgtccggcag aaaggcgcac
    11341 gtaagaggtc ggcatcatga tccgcgcgac cgcaatggtg cgaataaaat caaaggcatc
    11401 aacgtcatcg ttatcggcaa gcggcgtgcc tttcaccttc accagcatgt tgattggcac
    11461 gctttccggc ggcgtcggca ggtttgccag ttgccgcaat aatccggcgc gatcttttac
    11521 cgtttcgcct aagcccacaa tgccgccaga acagactttg atcccggcat cgcgcacttt
    11581 ttccagcgta tcgaggcgtt cctgataagt gcgggtggtg atgatattgc cgtaaaactc
    11641 cggcgaggtg tccaggttgt ggttgtagta atccagcccg gcgttcgcga ggcgctgcgc
    11701 ctgagattca ctcaacgtgc ccagcgtcat acacgcctcc agccccatcg cttttacccc
    11761 ctgcaccatt tgttccaggt acggcatatc gcgttcgtgg ggattcttcc acgccgcgcc
    11821 catacagaag cgcgtcgatc ctgccgcttt cgctttgcgc gccgactcca gcacctgttc
    11881 aacttccatc aaccgctcgg cttccagccc ggttttgtag cgcgagcttt gcgggcagta
    11941 tttgcaatct tccggacaag ctccggtctt aatcgacagc aacgtgctga cctgcacctg
    12001 acgaggatcg aaatgctggc gatgcacctg ctgcgcttca aacagcagat ccagcaacgg
    12061 tttttcaaat aattctgtga cttgcgacaa tgtccagcgt gggcggtgag ccatggggct
    12121 tctccaaaac gtgttttttg ttgttaattc ggtgtagact tgtaaaccta aatcttttca
    12181 atttggttta caagtcgatt atgacaacgg acgatcttgc ctttgaccaa cgccatatct
    12241 ggcacccata cacatccatg acctcccctc taccggttta tccggtggcg agcgccgaag
    12301 gttgcgagct aattttgtct gacggcagac gcctggttga cggtatgtcg tcctggtggg
    12361 cggcgatcca cggctacaat cacccacagc ttaatgcggc gatgaagtcg caaattgatg
    12421 ccatgtcgca tgtgatgttt ggcggtatca cccatacgcc agccattgag ctgtgccgca
    12481 aactggtggc gatgacgccg caaccgctgg agtgcgtttt tcttgcggac tccggttccg
    12541 tagcggtgga agtggcgatg aaaatggcgt tgcagtactg gcaagccaaa ggcgaagcgc
    12601 gccagcgttt tctgaccttc cgcaatggtt atcatggcga tacctttggc gcgatgtcgg
    12661 tgtgcgatcc ggataactca atgcacagtc tgtggaaagg ctacctgcca gaaaacctgt
    12721 ttgctcccgc cccgcaaagc cgcatggatg gcgaatggga tgagcgcgat atggtgggct
    12781 ttgcccgcct gatggcggcg catcgtcatg aaatcgcggc ggtgatcatt gagccgattg
    12841 tccagggcgc aggcgggatg cgcatgtacc atccggaatg gttaaaacga atccgcaaaa
    12901 tgtgcgatcg cgaaggtatc ttgctgattg ccgacgagat cgccaccgga tttggtcgta
    12961 ccggcaaact gtttgcctgt gaatatgcag aaatcgcgcc ggacattttg tgcctcggta
    13021 aagccttaac cggcggcaca atgacccttt ccgccacact caccacgcgc gaggttgcag
    13081 aaaccatcag taacggcgaa gccggctgct ttatgcacgg gccaactttt atgggcaatc
    13141 cgctggcctg cgcggctgca aacgccagcc tggcgattct cgaatctggc gactggcagc
    13201 aacaggtggc ggatattgaa gtacagctgc gcgagcaact tgcccccgcc cgtaatgccg
    13261 aaatggttgc cgatgtgcgc gtgctggggg caatcggcgt agtcgaaaca actcgtccgg
    13321 tgaatatggc ggcgctgcaa aaattctttg tcgaacaggg tgtctggatc cggccttttg
    13381 gcaaactgat ttacctgatg ccgccctata ttattctccc gcaacagttg cagcgtctga
    13441 ccgcagcggt taaccgtgcg gtgcaggatg aaacattttt ttgccaataa cgggaagtca
    13501 gcgtgtgggt ttctggctac actttctgca aactagaaag gagggttcat gaaactcatc
    13561 agtaatgatc tgcgcgatgg cgataagttg ccgcatcgtc atgtctttaa cggcatgggt
    13621 tacgatggcg ataatatttc accgcatctg gcgtgggatg atgttcctgc gggaacgaaa
    13681 agttttgtcg tcacctgcta tgacccggat gcgccaaccg gctccggctg gtggcactgg
    13741 gtagttgtta acttacccgc tgatacccgc gtattaccgc aagggtttgg ctctggtctg
    13801 gtagcaatgc cagacggcgt tttgcagacg cgtaccgact ttggtaaaac cgggtacgat
    13861 ggcgcagcac cgccgaaagg cgaaactcat cgctacattt ttaccgttca cgcgctggat
    13921 atagaacgta ttgatgtcga tgaaggtgcc agcggcgcga tggtcgggtt taacgttcat
    13981 ttccactctc tggcaagcgc ctcgattact gcgatgttta gttaatcact ctgccagatg
    14041 gcgcaatgcc atctggtatc acttaaaggt attaaaaaca actttttgtc tttttacctt
    14101 cccgtttcgc tcaagttagt ataaaaaagc aggcttcaac ggattcattt ttctatttca
    14161 tagcccggag caacctgtga acacattttc agtttcccgt ctggcgctgg cattggcttt
    14221 tggcgtgacg ctgaccgcct gtagctcaac cccgcccgat caacgtcctt ctgatcaaac
    14281 cgcgcctggt acctcttctc gcccgattct gtcggcaaaa gaagcgcaga atttcgatgc
    14341 tcaacactat tttgcatccc tgacaccagg tgctgcagcg tggaatcctt ccccgattac
    14401 cctgcctgcg caacctgact ttgttgtcgg cccggcgggc actcaaggtg taacgcatac
    14461 cacgattcag gcggcggtag atgcggcaat tatcaagcgt accaacaagc gccagtatat
    14521 tgccgtgatg cctggtgagt atcagggaac ggtatatgtc cctgccgctc cgggtggaat
    14581 tactctgtac ggtacaggtg aaaaaccgat tgatgtgaag attgggcttt cccttgatgg
    14641 tggcatgagc cctgccgact ggcgtcacga cgtcaacccg cgcggcaaat atatgccagg
    14701 taaaccagcg tggtatatgt acgatagctg ccagagcaaa cgcagcgaca gtatcggtgt
    14761 tctctgctct gcggtcttct ggtcacaaaa caatggcctg caactgcaaa atctgaccat
    14821 cgaaaacacg ctgggcgata gcgtagatgc aggtaaccat ccggcggtgg cactgcgtac
    14881 tgatggtgac caggtacaga ttaacaacgt taacattctc ggtcgtcaga acaccttctt
    14941 tgtcaccaac agcggtgtgc agaaccgtct ggaaaccaac cgtcagccgc gtacgctggt
    15001 gaccaacagc tacattgaag gggatgtgga tatcgtttct ggtcgcggcg cagtggtgtt
    15061 cgataacacc gaattccgcg tggtgaactc acgtactcag caagaagcgt atgtgtttgc
    15121 accggctacg ctgtccaaca tttactacgg tttcctcgcc gtaaacagcc gtttcaatgc
    15181 tttcggtgat ggtgtggcgc aactgggccg ctcgctggat gttgatgcca ataccaacgg
    15241 tcaggtggtg atccgtgata gcgccatcaa cgaaggtttt aacacggcta aaccgtgggc
    15301 cgatgcggtg atctctaatc gtccgtttgc gggtaatacc ggcagcgtag atgataacga
    15361 cgaaatacag cgcaatctga atgacactaa ctacaaccgc atgtgggaat acaataaccg
    15421 cggcgtgggt agtaaagtgg ttgcagaggc gaagaagtaa gagcaattaa ctatttgccg
    15481 gatgcggcgt aaacgcctta tccggcctac ggttcgatgc gatttgtagg tcggataaga
    15541 tgcgcaagca tcgcatccga caataagtgc cggatgctgc gaaaatgcct tatctggcct
    15601 acagattcga tgcgattcgt aggtcggata agatgcgcaa gcatcgcatc cgacaataag
    15661 tgccgaatgc gacctacatt cacatggcgc tttttacatc tgacggtttt tattgaagtt
    15721 aatcaaacta cccgccttga taatctcgcg ctcttcagca gtcagacttt ccatatagag
    15781 cgtaatttcc gttaccggcg catcttcatg gatcacataa cctttaaacg tcgtacccgg
    15841 attatccagc gccgctttaa tgccagggat gtaaatgtaa tcccccactt caaaggttgg
    15901 cacttccgcc atctgcagcg gtaacatccc ccagttgatg acgttagaac ggtagcgttt
    15961 agtcgcgtac tcttcggcaa tattcgccag accgccaatc acacgctggc agctcgccgc
    16021 ctgttcacgc gcagaaccat cgcctggttt caccgcatat accatgctgc caatttcagt
    16081 ttgcagcgga tcaatatgct cctgaccagc aatctgctta atgcgcgcaa acacctctgt
    16141 cagctcgctg acattccccg ccagacgctg attttccagc tcagcagtcg ctttacttct
    16201 gccaacataa ccgggatcgc ggcgtgacag ggtaaactcc gccagaccaa tcggatttga
    16261 acgataagaa gaggtttcac cggaaggaat cagttcgtcg gtggtggtca cttcgtcgag
    16321 gatcttcgag cacactttca ggacgatatt gtcagtcagc gcacccaatt ccggccagtc
    16381 tttaatgttc ggtccgtaaa tcagcggttg ctgagttgcc cctttcacaa agccctgata
    16441 aacacggttt ttatacggcg ttacatcgaa ggcgtactcc ggcacgttgt cccagcaatc
    16501 aagttcgctg gcagaggtta aatagccacc gtttgccgca gtcgcagcga tagaacgagc
    16561 gtccatcaac gccaccgctg acatctgccc attagctggc ttagagcctt cgcggttcgg
    16621 gaagttacgc gtggtgtggc gaatacttaa tccgttgttg attggcgtat cgcccgcgcc
    16681 aaagcatggg ccgcagaacg cggttctgat gattgcgcct gcgccaatca aatctgctac
    16741 cacacctttt ttggcgagat ccataaacac cggctgtgat gacgggtaaa ctgccagcga
    16801 gaaggtgtca ttgccacagg attgaccgcg cagtgcattc gccgccgcga tgacgttttc
    16861 gtagttaccg ccagaacagc ccgcgataat cccctgctgc actttcaggc gaccattttc
    16921 cactttatcc agcagcgaga gtttggcttt accgtgcgcc acgcgttcgg actcaatttc
    16981 aatctcacgc agaatgtcgg tcaggttctg gttcagtgtg tcgatttcat acacgttgct
    17041 cgggtggaac ggcagcgcaa tcattggttt gatggcgctt aaatcaacgc tgatgcagcc
    17101 atcgtagtac gccatcggtt gagggttaag ctggcagtaa tcctggccgc gaccgtgcag
    17161 cgccagccag ttatgcactt cttcatcagt ttgccagacg gagcttaaac aggtggtttc
    17221 cgtggtcatc acgtcaacgc tgttacggaa atcggtagag agcgcgctaa cgcccggtcc
    17281 aacgaactcc atgactttgt ttttgacgta accgtttttg aacaccgcgc caatgatagc
    17341 cagcgccaca tcctgcggcc ccacatacgg cgctggtttt ccggtcagat gcaccgcaac
    17401 aactcccgga tagtcgatat cccaggtgtc attaagcagc tgttttacca actcaccacc
    17461 gccctcaccg actgccattg tccctaatgc accgtaacgg gtgtggctgt ctgacccgag
    17521 gatcattttg ccgccgcctg ccatcatctc acgcatatat tgatggatga ccgcaatatg
    17581 cggaggcaca aaaataccgc cataacgctg agctgccgat aaaccaaaaa catggtcatc
    17641 accgttaatg gtgccgccga cggcgcagag tgagttatgg cagttggtca gcacatacgg
    17701 cagcgggaaa cgttccatac cggacgcttt agcggtctgt acaataccaa caaaggtaat
    17761 atcgtgagag gctaatgaat caaacttaat tttaagttta tccatatttc cggacgtatt
    17821 atgcgaagag agaatagacc aggcaatagt gccttttttg gcttcttctt ttttaatttc
    17881 gccggtgaaa tgttcttcag caattatttc gttattactg gcgagaaaca cgcctttttc
    17941 agataacttg atcatcaaat gctccagata tctgcgcacc gtgaagtgcg cagaaagata
    18001 ataaatggat atactctaaa taatttgagt tgcaggaagg cgacaagcga gtgaatcccc
    18061 aagagcttac ataagtaagt gactggggtg agcgaa
//
LOCUS       DAPHDW010000088        17863 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.089, whole genome shotgun sequence.
ACCESSION   DAPHDW010000088 DAPHDW010000000
VERSION     DAPHDW010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.089"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            70..411
                     /gene="symE"
                     /locus_tag="RH057_002972"
     CDS             70..411
                     /gene="symE"
                     /locus_tag="RH057_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HDV3129311.1"
                     /translation="MTDTHSIAQPFEAEVSPANNRQLTVSYASRYPDCSRIPAITLKG
                     QWLEAAGFATGTVVDVKVMEGCIVLTAQPPVAEESELMQSLRQVCKLSARKQKQVQEF
                     IGVIAGKQKVA"
     gene            complement(458..1621)
                     /locus_tag="RH057_002973"
     CDS             complement(458..1621)
                     /locus_tag="RH057_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1524 domain-containing protein"
                     /protein_id="HDV3129312.1"
                     /translation="MALGKESDKSLATAFQDLRELKVDVAYPFLLALYHDYKNGVLSH
                     EDFLSIIRLIESYVFRRAVCAIPTNSLNKTFATFYKVINKEKYLESIQVHFLNLPSYR
                     RFPNDDEFKRELKVRDLYNFRSRSYWLRRLENDKRRERVEEFTIEHIMPQNENLSAKW
                     REELGSDWQRIHKELLHTLGNLTLTRYNSRYSDRPFAEKRDIEDGFKHSPLYLNIGLG
                     QCEKWDEAAIHARADRLAELAVQVWQAPSLPEEVLAVYRGQPENKTSYSLSDYPFLAD
                     GLHSRLLFDHLRDEIMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLRLSLNMQFH
                     ELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMESALV"
     gene            complement(1669..2550)
                     /locus_tag="RH057_002974"
                     /pseudo
     CDS             complement(1669..2550)
                     /locus_tag="RH057_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
     gene            complement(2647..2811)
                     /locus_tag="RH057_002975"
     CDS             complement(2647..2811)
                     /locus_tag="RH057_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HDV3129313.1"
                     /translation="MEFHENKAKTPFIRLVQLWQAMRRWRRQMQTRRVLQQMSDERLR
                     DIGLRREDVE"
     gene            2988..4400
                     /locus_tag="RH057_002976"
     CDS             2988..4400
                     /locus_tag="RH057_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV3129314.1"
                     /translation="MTRYQHLATLLAERIEQGLYRHGEKLPSVRSLSQEHGVSISTVQ
                     QAYQTLETMKLITPQPRSGYFVAQRKAQPPVPPMTRPVQRPVEITQWDQVLDMLEAHS
                     DSSIVPLSKSTPDVETPSLKPLWRELSRVVQHNLQTVLGYDLLAGQRVLREQIARLML
                     DSGSVVTADDIIITSGCHNSMSMALMAVCKPGDIVAVESPCYYGSMQMLRGMGVKVIE
                     IPTDPETGISVEALELALEQWPIKGIILVPNCNNPLGFIMPDARKRAVLSLAQRHDIV
                     IFEDDVYGELATEYPRPRTIHSWDIDGRVLLCSSFSKSIAPGLRVGWVAPGRYHDKLL
                     HMKYAISSFNVPSTQMAAATFVLEGHYHRHIRRMRQTYQRNLALYTCWIREYFPCEIC
                     ITRPKGGFLLWVELPEQVDMVCVARQLYRMKIQVAAGSIFSASGKYRNCLRINCALPL
                     SETYREALKQIGEAVYQAME"
     gene            complement(4642..5598)
                     /locus_tag="RH057_002977"
     CDS             complement(4642..5598)
                     /locus_tag="RH057_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001678496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rpn family recombination-promoting
                     nuclease/putative transposase"
                     /protein_id="HDV3129315.1"
                     /translation="MTNFTTSTPHDALFKSFLTHPDTARDFMEIHLPKDLRELCDLDS
                     LKLESASFVDEKLRALHSDILWSVKTREGDGYIYVVIEHQSREDIHMAFRLMRYSMAV
                     MQRHIEHDKRRPLPLVIPMLFYHGSRSPYPWSLCWLDEFADPTTARKLYSAAFPLVDV
                     TVVPDDEIVQHRRVALLELIQKHIRQRDLMGLIDQLVVLLVTEFANDSQITALLNYIL
                     LTGDEARFKAFISELTRRMPHHRERIMTIAERIHNDGWLLGRERGRKEGKVEGERSLL
                     RLLLQNGADPEWIQRYTGLSAEQMQALDQPLPESKREPWIEY"
     gene            6065..7297
                     /gene="mdtM"
                     /locus_tag="RH057_002978"
     CDS             6065..7297
                     /gene="mdtM"
                     /locus_tag="RH057_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtM"
                     /protein_id="HDV3129316.1"
                     /translation="MPRFFARHAATLFFPMALILYDFAAYLSTDLIQPGIINVVRDFN
                     ADVSLAPAAVSLYLAGGMALQWLLGPLSDRIGRRPVLITGALIFTLACAATMFTTSMT
                     QFLIARAIQGTSICFIATVGYVTVQEAFGQTKGIKLMAIITSIVLIAPIIGPLSGAAL
                     MHFVHWKVLFAIIAVMGFISFVGLLLAMPETVKRGAVPFSAKSVFRDFRNVFCNRLFL
                     FGAATISLSYIPMMSWVAVSPVILIDAGGLTTSQFAWTQVPVFGAVIVANAIVARFVK
                     DPTEPRFIWRAVPIQLVGLALLIIGNLLSPHVWLWSVLGTSLYAFGIGLIFPTLFRFT
                     LFSNNLPKGTVSASLNMVILMVMSVSVEIGRWLWFNGGRLPFHLLAVVAGVIVVFTLA
                     GLLNRVRQHQAAELAEER"
     gene            7338..8618
                     /locus_tag="RH057_002979"
     CDS             7338..8618
                     /locus_tag="RH057_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HDV3129317.1"
                     /translation="MNKLIELRRAKMLALSLLLIAAATFVVTLFLPPNFWVSGVKAIA
                     EAAMVGALADWFAVVALFRRVPIPIISRHTAIILRNKDRIGENLGQFVQEKFLDTQSL
                     VALIRRHEPALLIGNWFSQPENARRVGQHLLQIMSGFLELTDDARIQRLLKRAVHRAI
                     DKVDLSGTSALMLESMTKNDRHQVLLDTLIAQLIALLQRDKSRKFIAQQIVRWLESEH
                     PLKAKILPTEWLGEHSAELVSDAVNSLLDDISRDRSHQIRHAFDRATFALIDKLKNDP
                     EMAARADAVKSYLKEDEAFNRYLSELWGDLREWLKADINSEDSRVKERIARAGQWFGE
                     TLIADDALRASLNGHLEQAAHRVAPEFSAFLTRHISDTVKSWDARDMSRQIELNIGKD
                     LQFIRVNGTLVGGCIGLILYLLSQLPALFPLGNF"
     gene            8734..9885
                     /locus_tag="RH057_002980"
     CDS             8734..9885
                     /locus_tag="RH057_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacyl-CoA dehydratase subunit D"
                     /protein_id="HDV3129318.1"
                     /translation="MSLVTDLPAIFDQFSEARQKGFLTVMDLKERGIPLVGTYCTFMP
                     QEIPMAAGAVVVSLCSTSDETIEEAEKDLPRNLCPLIKSSYGFGKTDKCPYFYFSDLV
                     VGETTCDGKKKMYEYMAEFKPVHVMQLPNSVKDDTSRALWKAEMLRLQKTVEERFGHE
                     ISEDALRDAIALKNRERRALANFYHLGQLNPPALSGSDILKVVYGATFRFDKEALINE
                     LDAMTARVRQQWEEGQRLDPRPRILITGCPIGGAAEKVVRAIEENGGWVVGYENCTGA
                     KATEQCVAETGDVYDALADKYLAIGCSCVSPNDQRLQMLSQMVEEYQVDGVVDVILQA
                     CHTYAVESLAIKRHVRQQHNIPYIAIETDYSTSDVGQLSTRVAAFIEML"
     gene            9895..10662
                     /locus_tag="RH057_002981"
     CDS             9895..10662
                     /locus_tag="RH057_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129319.1"
                     /translation="MTYSIGIDSGSTATKGILLADGVITRRFLVPTPFRPATAITEAW
                     ETLREGLETTPFLTLTGYGRQLVDFADKQVTEISCHGLGARFLAPATRAVIDIGGQDS
                     KVIQLDDDGNLCDFLMNDKCAAGTGRFLEVISRTLGTSVEQLDSITENVTPHAITSMC
                     TVFAESEVISLRSAGVAPEAILAGVINAMARRSANFIARLSCEAPILFTGGVSHCQTF
                     ARMLESHLGMPVNTHPDAQFAGAIGAAVIGQRVRTRR"
     gene            10659..10916
                     /locus_tag="RH057_002982"
     CDS             10659..10916
                     /locus_tag="RH057_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3343 domain-containing protein"
                     /protein_id="HDV3129320.1"
                     /translation="MKEFLFLFHSTVGVIQTRKALQAAGMTFRVSDIPRDLRGGCGLC
                     IWLTCPPGEEIQWVIPGLTESIYCQQDGVWRCIAHYRVSPR"
     gene            10981..11841
                     /locus_tag="RH057_002983"
     CDS             10981..11841
                     /locus_tag="RH057_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418753.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiK family protein"
                     /protein_id="HDV3129321.1"
                     /translation="MTKSISLSKRISVIVILFAIVAVCTFFVQSCARKSNHAASFQNY
                     HATIDGKEIAGITNNISSLTWSAQSNTLFSTINKPAAIVEMTTNGDFIRTIPLDFVKD
                     LETIEYIGDNQFVISDERDYAIYVISLTPNSEVKILKKIKIPLQDSPTNCGFEGLAYS
                     RQDHTFWFFKEKNPIEVYKVNGLLSSNELHISKDEALQRQFTLDDVSGAEFNQQKNTL
                     LVLSHESRALQEVTLVGEVIGEISLTKGSRGLSHNIKQAEGIAMDASGNIYIVSEPNR
                     FYRFTPKSSH"
     gene            11909..13087
                     /locus_tag="RH057_002984"
     CDS             11909..13087
                     /locus_tag="RH057_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3129322.1"
                     /translation="MPSSSHPVERFSFSTALFGMLVLTLGMGLGRFLYTPMLPVMMAE
                     GAFSFSQLSWIASGNYAGYLAGSLLFSFGAFHQPSRLRPFLLVSALASGLLILAMAWL
                     PPFILVLLIRFLAGVASAGMLIFGSTLIMQHTRHPFVLAALFSGVGIGIALGNEYVLA
                     GLHFAFSSQTLWQGAGALSGMMLIALTLLMPSKKHAIAPMPLAKTEQQIMSWWLLAIL
                     YGLAGFGYIIVATYLPLMAKDAGSPLLTAHLWTLVGLSIVPGCFGWLWAAKRWGALPC
                     LTANLLVQAISVLLTLASDSPLLLIISSIGFGGTFMGTTSLVMTIARQLSVPGNLNLL
                     GFVTLIYGIGQILGPALTSMLGNGTSALAGATLCGAAALFIAALISTVQLFKLQVVTS
                     "
     gene            complement(13100..13654)
                     /gene="kptA"
                     /locus_tag="RH057_002985"
     CDS             complement(13100..13654)
                     /gene="kptA"
                     /locus_tag="RH057_002985"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HDV3129323.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADIDKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGERHYVHLSADEATARKVGARHGSPVILTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEW"
     gene            13904..14587
                     /locus_tag="RH057_002986"
     CDS             13904..14587
                     /locus_tag="RH057_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129324.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFSGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGAGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            14584..15045
                     /locus_tag="RH057_002987"
     CDS             14584..15045
                     /locus_tag="RH057_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HDV3129325.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATAGALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            15058..16230
                     /gene="iadA"
                     /locus_tag="RH057_002988"
     CDS             15058..16230
                     /gene="iadA"
                     /locus_tag="RH057_002988"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV3129326.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPDCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALQPVYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNLTGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVNGTFETA"
     gene            16295..17206
                     /gene="hypT"
                     /locus_tag="RH057_002989"
                     /gene_synonym="qseD"
     CDS             16295..17206
                     /gene="hypT"
                     /locus_tag="RH057_002989"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HDV3129327.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMGRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGKLVVLNRDELVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(17199..17591)
                     /gene="iraD"
                     /locus_tag="RH057_002990"
     CDS             complement(17199..17591)
                     /gene="iraD"
                     /locus_tag="RH057_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HDV3129328.1"
                     /translation="MMRQSLQAVLPEISGNKTSLLRKSVCSDLLTLFNSPHSALPSLL
                     VSGMPEWQVHNQSDKHLQSWYCRQLRSALLFHEPRIAALQVNFKEAYCHILAISLEIM
                     LYHDGEPLTFDLVWDNGGWCSAMLENVS"
     gene            complement(17588..17671)
                     /gene="idlP"
                     /locus_tag="RH057_002991"
     CDS             complement(17588..17671)
                     /gene="idlP"
                     /locus_tag="RH057_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iraD leader peptide IdlP"
                     /protein_id="HDV3129329.1"
                     /translation="MENEHQYSGARCSGQAAYVAKRQECAK"
BASE COUNT         4169 a         4542 c         4478 g         4674 t
ORIGIN      
        1 cgcggtgggg atgtgcgcca acacacccgc accgctcatc acaatcacta ttttcaagga
       61 atagcagtta tgactgacac gcattctatt gcacaaccgt tcgaagcaga agtctccccg
      121 gcaaataacc gtcaattaac cgtgagttat gcgagccgct acccggattg cagccgtatt
      181 cccgccatca ccctgaaagg tcagtggctg gaagccgccg gttttgccac cggcactgtg
      241 gtagatgtca aagtgatgga aggctgtatt gtcctcaccg cccaaccccc cgtcgccgaa
      301 gagagcgaac tgatgcagtc actgcgccag gtgtgcaagc tgtcggcacg taaacagaag
      361 caggtgcagg aatttattgg ggtgattgcc ggtaaacaga aagttgcctg atacattgac
      421 ccggtagcac cagtgctacc gggtattatt cgccgcgtta taccaaggcg ctctccattt
      481 gtttttcaaa tgcctgacga attaatccca taatgtaagg aagttgtgcg aggtcgctga
      541 aaccaatttc cacatcacca ttaccccagc gcccaacatt tgtcacatct ttggcaatac
      601 ctttcggatc gaccagttca tgaaactgca tattaagcga caatcgcagt cggctttttt
      661 gcggcacaac atcaacaaaa ttcgtttcag ctttaaacgc aatatagagc ttcaatactt
      721 cctgcgtgat ccctgcgtcc aggcgcataa tttcatcgcg aagatgatca aataacaacc
      781 ggctatgcaa gccatcagca agaaaaggat aatcactcag gctgtaactg gttttgttct
      841 ccggctgtcc ccgataaaca gctaaaacct cttcaggaag agaaggcgct tgccagacct
      901 gaaccgcaag ctccgccaga cgatcggctc gggcgtgaat ggcagcttca tcccattttt
      961 cgcactgtcc aagaccgata ttcaaataga gcgggctatg cttaaagccg tcttcaatat
     1021 cgcgtttttc tgcgaaaggt ctgtcactgt aacgggagtt atagcgcgtt aaagtgagat
     1081 tccccaacgt atgcaacagt tctttatgaa tacgctgcca gtcgcttccc agctcttcgc
     1141 gccatttagc cgacaaattt tcgttctgcg gcataatatg ttcaatcgta aactcttcca
     1201 cgcgctctct gcgtttatcg ttttccagtc gtcgtaacca gtagctgcga ctacggaagt
     1261 tatagagatc gcgaactttt aattcccgtt taaattcatc atcgttgggg aaacgacgat
     1321 atgaaggtag attcaaaaaa tgtacctgaa tactttccag atatttttct ttattaatga
     1381 ccttataaaa agtagcaaac gtcttgttaa gagaattcgt cggaatcgca catactgcac
     1441 ggcggaaaac ataagattca attaaacgaa ttatgctcag gaaatcctcg tgagacaaaa
     1501 cgccattttt atagtcatga taaagcgcca gtaagaaagg atacgccaca tcgaccttta
     1561 actcgcgcaa atcctgaaaa gccgtagcaa gcgatttgtc actttctttt cccaatgcca
     1621 ttgcacagta atactccgcg tagatgtgaa tatcttcaac cagtcgatct acgccttttt
     1681 cagcaacact ctggctgcgg gcatggagtt taaatgcctc atagacttca tctgtccgag
     1741 ggatctcccc cgtttttacc gtcagataat gacgcataaa actgtcaaaa tattcgctgt
     1801 aaccttgctg accaaaggcg acttccattg gacgccagtg atcttcatac aaccgggttt
     1861 gatgctctgg ttcgaggccc atcagaataa aattgcgcac cagatcggcc tgacttaacg
     1921 ccttaccggt ggagttcata ctttcaaaaa tcagttgcgg attatcctga ccacgattaa
     1981 gcgccacatc gacaattaat aactttgcta acccacgaca taaggggatc aagtcatcac
     2041 cgagtttggc aatttgttca tcaaagaaag taaagtttcc attattcgat gggaatagtt
     2101 ttctggcatt ggtctttgtt ttattaacgc cagcaaactg tctttatcag tctcggtcag
     2161 cagtaattta aaacctttct cgccggactc ataaggattg agcaaataat aattacgcaa
     2221 tttcattgcc gaaaagccat caaaaacttc gtcttcgcca agatggcgcg ataaagcctc
     2281 aatcagcaac attgccgtcg tcagccgttg ttgaccatca atgaccagca acggagaaat
     2341 accagaaacc tgatacaacc cctgctctat ataaacaacc gaaccgataa aatgcgctga
     2401 tatatcgtca cgctttcctg cacgaatgat gtcgtcccaa agttgccgac attgttgttc
     2461 tgtccatgaa taagtccgtt gataaatggg aataacaaac tgctgcgaac gttttaaaaa
     2521 atcgagtagg cgagcttctg tagctttcat tctgacgtct ttccgtgaaa cgatgatttc
     2581 gcaataataa ccacttcctt gtggaggtaa tagagaaatc ctgtttatcc gcgacgaaca
     2641 tacccctcac tccacatcct ccctgcgtaa cccgatatcc ctcaaccgct catcactcat
     2701 ctgctgtaac actcgtcggg tctgcatttg ccgccgccaa cgcctcatcg cctgccagag
     2761 ttgcacaagt ctgataaacg gcgttttggc tttgttttcg tgaaattcca tctgctgcct
     2821 cctcatcttt cagaggcttc atcatccgcc ttctggcagt tcacaataca gatgcaaata
     2881 ttagttttat ttaacataca gacaggttaa aacagcatct gcatccggtt ttttagcttc
     2941 atctgtaccg gtttttctct ctgtatggtc taaataagga atacagcatg acgcgttatc
     3001 aacatctggc aactctgctt gccgagcgga ttgagcaagg gctgtatcgt cacggggaga
     3061 aattgccgtc ggtgcgcagc ttaagtcagg agcacggcgt cagcatcagc accgtgcagc
     3121 aggcgtacca gacgctggag acgatgaagc tcatcactcc gcagccgcgt tcgggttatt
     3181 ttgtcgcaca acggaaagcc caaccgccag tgccaccaat gacgcgtccg gtgcagcgcc
     3241 cggtggaaat tacccagtgg gatcaagtgc tggatatgct agaggcgcat agcgacagtt
     3301 ccattgttcc gttaagcaaa agcacgccgg atgtcgaaac gcccagcctg aaaccactct
     3361 ggcgggagct aagccgggtg gtacagcata atctgcaaac cgttctcggt tatgacttgt
     3421 tagccggtca gcgagtgttg cgagagcaaa ttgcccgcct gatgctcgac agcggctcgg
     3481 tggtcaccgc cgatgacatc atcatcacca gcggctgcca taactcgatg tcgatggcgt
     3541 taatggcggt gtgtaaacca ggcgatattg tcgcggtcga atctccctgt tattacggtt
     3601 cgatgcagat gctgcgaggc atgggcgtga aagtgattga aatcccaacc gatccagaaa
     3661 ctggcatcag cgttgaagca ctggaactgg cgttggaaca gtggccgatt aaaggcatca
     3721 ttctggtgcc aaactgtaat aatccgctgg gatttattat gccggacgca cgcaaacggg
     3781 ccgttctctc tctcgctcag cgtcatgata ttgtgatttt tgaagatgat gtctacggcg
     3841 aactggcaac ggagtatccg cgcccgcgga ccatccattc atgggatatc gacgggcgag
     3901 tgttgttgtg cagctcgttc agtaaaagta ttgctccagg cctgcgcgtg ggttgggtcg
     3961 caccggggcg ctatcacgat aaactgctgc atatgaaata cgccatcagc agctttaatg
     4021 taccgtccac gcaaatggcg gcggcaacgt ttgtgctgga aggtcactat catcgccata
     4081 tccggcggat gcggcagact tatcagcgca atctggcgct ttatacctgc tggatacggg
     4141 aatattttcc ctgcgaaatc tgtattacgc gcccgaaagg cggattttta ctgtgggtag
     4201 aattgcctga acaggtcgat atggtctgcg tcgcgcggca gctgtaccgc atgaaaatcc
     4261 aggtggcggc aggctcgatt ttctcagcgt ccggcaaata ccgtaattgt ctgcgcatca
     4321 actgcgcttt accgctcagc gaaacctatc gcgaagcact aaagcaaatt ggcgaggccg
     4381 tgtatcaggc aatggaatga attcgaaata gtggcgaagc acctgattta ctttcacgaa
     4441 tcgcctgtaa ctcgttttat ctttatagcg acatcgccac gttccggcat agcttaggca
     4501 aagtgtgctt tgttcatgcc ggatgcggcg tgaacgcctt atccggccta caaaaccagc
     4561 aaattcaata gattgcagag gtaatgtagg tctgataagc gtagcgcatc aggctgcttt
     4621 gcgtttgtca tcagcctctg attagtactc gatccatggt tcgcgcttgc tttcaggcaa
     4681 gggctgatct aatgcctgca tttgctctgc cgaaagtccg gtatatcgtt gtatccattc
     4741 aggatcggcc ccattctgca acaacaatcg gaggaggctc cgttcccctt ctactttccc
     4801 ttctttcctc cccctctccc ttcccaacag ccatccatca ttatgaattc gctctgcaat
     4861 tgtcattatt cgctccctgt ggtgtggcat tcgcctggta agttcgctga taaacgcctt
     4921 aaaacgcgct tcatcgccag tcagtaaaat gtaatttaac agcgcagtta tctggctgtc
     4981 attagcaaac tctgtaacca gtaatactac cagttggtcg ataagcccca tcagatcgcg
     5041 ctggcgaata tgcttttgga tcaactccaa cagggcgact ctgcgatgct gcacaatctc
     5101 gtcgtctggc acgacagtga catccaccag cgggaacgct gcgctataaa gtttccgtgc
     5161 ggtagtcggg tcggcaaatt cgtccagcca gcacagggac cagggataag gactacggct
     5221 accgtgataa aatagcatcg ggatgaccag cggtagcggc cgacgtttat catgctcgat
     5281 atggcgctgc atcaccgcca tggaatagcg catcaggcga aaggccatat gaatgtcctc
     5341 gcggctctga tgttcaatca ccacatagat ataaccatcg ccttcgcggg tctttaccga
     5401 ccacagaata tcggaatgta gcgcccgcaa tttttcatcg acaaagctgg cggattccag
     5461 ttttaagctg tcgagatcgc acagttcacg taaatcttta ggtaagtgga tctccataaa
     5521 atcccgcgcg gtgtcagggt gcgtgagaaa ggacttaaat aatgcgtcat gcggcgtgct
     5581 ggttgtgaag tttgtcatgg cgatccgtca cctgctaata agatggcccg actgtagcga
     5641 ctaacaaaac cattctcacc cggcaatttc tcgttccccg aggcactttc caggctattt
     5701 ttgttcgtct gcaaacgaaa catttttccg gaaacaggct cgcgttttac atttcctgta
     5761 ccaggcagat attccccttt ccaccgcgct cttttgatta accgcaaatt ttaattacac
     5821 ttaaggtgta tattttctat gcaacccatc aattcaagag gtgtaatgtg ctgatgacta
     5881 tttgtaatcg ttatacattc tgacctggag tcagaaggta tttctctgtc tgtgtgttca
     5941 caggcagtgt ggttgattac atgaaattca gtacatttgt ccttctcgcc tgcctttcgt
     6001 cattaccgac ggtattgaat ttcgttttcc ccgttggggt tctccggaca aggagttgtt
     6061 tgttatgcca cgtttttttg cccgccatgc cgccacgctg tttttcccga tggcgttgat
     6121 tttgtatgac tttgccgcgt acctgtcgac ggatctgatc cagccgggga tcattaatgt
     6181 ggtccgtgat tttaatgccg atgtcagtct ggccccggca gccgtcagtc tctatctcgc
     6241 gggcggtatg gcgttacagt ggctgctggg gccgctttcc gacaggattg gccgcaggcc
     6301 ggtgctgatt accggggcgc taatatttac ccttgcctgc gctgcgacaa tgttcacaac
     6361 gtctatgaca cagtttctta tcgcgcgtgc aattcagggc accagtatct gttttattgc
     6421 caccgttggt tatgtcacgg tgcaggaggc gttcggccag acaaaaggga tcaagttgat
     6481 ggcgattatc acctccatcg tactgattgc gccgattatt ggcccgcttt ccggcgcagc
     6541 tctgatgcac tttgtgcact ggaaagtcct ttttgccatc attgcagtta tgggttttat
     6601 ctcatttgtt ggcttactgc tggcgatgcc agagacggtg aagcgcggcg cggttccatt
     6661 tagcgccaaa agcgtctttc gcgattttcg taatgtcttt tgcaatcggc tgttcctctt
     6721 tggcgcagca accatctctt taagctatat cccaatgatg agttgggtgg ctgtctcgcc
     6781 ggtgatcctg atcgacgcgg gcggcttaac aacttcgcag ttcgcctgga cacaggttcc
     6841 ggtgttcggc gcggtgattg tcgcgaatgc catcgtggcg cgtttcgtta aagatccgac
     6901 cgaaccgcgc tttatctggc gtgctgtacc cattcaactg gtcggcctcg cactgttgat
     6961 tatcggcaat ctgctgtcac cgcacgtctg gctgtggtcg gtgctgggca ccagtttgta
     7021 tgctttcggg attggtttga tcttcccgac cttattccgc tttacgctgt tttccaataa
     7081 cttaccgaaa gggaccgtct ccgcatcgct gaatatggtg atcctgatgg tgatgtctgt
     7141 ctcggtcgaa atcggccgct ggctgtggtt taacggcggt cgcttgccgt ttcatctgtt
     7201 agccgtcgtg gcgggcgtta tcgtcgtttt caccctggca ggattgctta atcgcgtgcg
     7261 ccaacatcag gctgccgagc tggcggagga gcggtgattt ttgcgcgatc ctgccgtcag
     7321 gctctattct taacgttatg aataaactca ttgaactcag acgcgccaaa atgttggcgc
     7381 tctctttact gcttatcgcc gctgctacct ttgtcgttac gctgtttttg ccgcccaatt
     7441 tttgggtgag cggcgtgaag gcgattgctg aagcggcgat ggtcggcgcg ctggcggact
     7501 ggtttgcggt ggtggcgctg tttcgccgcg tgccgattcc gatcatttct cgtcatacgg
     7561 cgattatcct gcgtaataaa gaccggattg gcgaaaatct cggtcagttc gtgcaggaaa
     7621 aatttctcga tacccagtcg ctggtggcat tgattcgacg tcacgaaccg gcgttgttga
     7681 ttggcaactg gtttagtcag ccggagaacg cccgccgcgt tggtcagcat ctgttgcaga
     7741 tcatgagcgg ttttcttgaa ctgaccgatg atgcgcgtat tcagcgcctg cttaagcgcg
     7801 cggtccatcg ggcgattgat aaggtcgatc tttccggaac cagtgcgttg atgctggaga
     7861 gtatgaccaa aaacgatcgt catcaggtgc tgctcgatac gctgatcgca cagttgattg
     7921 ctcttctcca gcgcgataaa tcgcgcaagt ttattgccca gcaaattgtt cgctggctgg
     7981 agagcgagca tccactgaaa gccaaaattt tgcctactga atggctgggc gaacatagcg
     8041 cggagttggt ttctgacgcg gtgaattctt tgcttgatga tatcagccgt gatcgttcgc
     8101 atcagatccg ccatgcgttt gatcgcgcca cttttgccct gatcgacaag ttgaaaaacg
     8161 atcctgaaat ggcagcgcga gccgatgccg taaaaagcta tctgaaagaa gatgaagctt
     8221 ttaaccgcta tctcagtgaa ttgtgggggg atttacggga atggctgaaa gcggatatca
     8281 acagtgaaga ttctcgtgtg aaagaacgta tcgcgcgggc gggtcaatgg tttggcgaaa
     8341 cgttaattgc cgatgatgcc ttgcgggcgt cgttaaatgg tcatctggaa caagccgcgc
     8401 accgcgtcgc gcctgagttt tccgcattcc tgacgcgcca tatcagcgat acggtaaaaa
     8461 gctgggatgc gcgggatatg tcgcggcaaa tagagttaaa tatcggcaaa gatctgcagt
     8521 ttatccgtgt caacggtacg ctggttggcg gttgtattgg gctaatttta tatttgttgt
     8581 cgcagctccc ggccttgttc cccctcggca atttttagaa atccatgaaa aactaagtag
     8641 ctaaccgcga gagagatcaa ataaaccaca ctaaaagaat ggtaataatg tggcctcttt
     8701 tcattatctc ccgtggtacg gggaaggaaa atcatgtcac ttgtcaccga tctacccgcc
     8761 attttcgatc agttctctga agctcgccag aaaggctttc tcaccgtcat ggatctcaag
     8821 gagcgcggca ttccgctggt tggcacttac tgcaccttta tgccgcaaga gatcccgatg
     8881 gcagccggtg cggttgtggt ttcgctctgt tccacctctg atgaaaccat tgaagaagcg
     8941 gagaaagatc tgccgcgcaa cctctgcccg ctgattaaaa gcagctacgg cttcggcaaa
     9001 accgataaat gcccctactt ctacttttct gatctggtgg tcggtgaaac cacctgcgac
     9061 ggcaaaaaga aaatgtatga atacatggcg gagtttaagc ccgttcatgt gatgcagctg
     9121 ccgaacagcg ttaaggacga tacctcgcgt gcgttatgga aagccgagat gctgcgcttg
     9181 caaaaaacgg tagaagaacg ttttgggcac gagattagcg aagatgctct gcgcgatgcc
     9241 attgctctga aaaaccgcga acgtcgcgca ctggctaatt tttatcatct tgggcagtta
     9301 aatcctccgg cgcttagcgg cagcgacatt ctgaaagtgg tttacggcgc aacattccgg
     9361 ttcgataaag aggcgttgat caatgaactg gacgcgatga cagcccgcgt tcgtcagcag
     9421 tgggaagaag gccagcgact ggacccgcgt ccacgcattt taatcaccgg ctgcccgatt
     9481 ggcggcgcag cagaaaaagt ggtgcgcgcg attgaagaga atggcggctg ggttgtcggt
     9541 tatgaaaact gcaccggggc gaaagcgacc gagcaatgcg tggcagaaac gggcgatgtc
     9601 tacgacgcac tggcggataa atatctggcg attggctgct cctgtgtttc gccgaacgat
     9661 cagcgcctgc aaatgctcag ccagatggtg gaagaatatc aggtcgatgg cgtagttgat
     9721 gtgattttgc aggcgtgcca tacctacgcg gtggaatcgc tggcgattaa acgtcatgtg
     9781 cgtcagcagc acaacattcc ttatatcgct attgaaacag actactccac ctcagatgtc
     9841 gggcagctca gtacccgtgt cgcggccttt attgagatgc tgtaaggagt ggcagtgaca
     9901 tattcgattg gcattgattc tggttctacc gccaccaaag ggatcttact ggcagacggc
     9961 gtgattacgc gccgtttcct cgttccaaca ccctttcgtc cggcaacagc aattactgaa
    10021 gcctgggaaa ctctgcgcga agggttagag acaacgccgt ttctgacgct caccggctat
    10081 ggacggcaac tggtggattt tgccgataaa caagtgacgg aaatctcctg tcatgggctg
    10141 ggcgcacggt ttcttgcgcc agcaacgcgc gcggtaatcg acatcggtgg tcaggacagc
    10201 aaagtgattc agcttgatga tgacggtaac ctgtgcgatt tcctgatgaa tgacaaatgc
    10261 gcggcgggca ccgggcgttt cctggaggtg atctcgcgca cgcttggcac cagcgtcgag
    10321 caactcgaca gcattaccga aaatgtcacg ccgcacgcca tcacgagtat gtgcacagtg
    10381 tttgccgaat cagaagtgat cagcctgcgc tcagcgggcg tcgcgccaga agcgattctc
    10441 gccggagtga ttaacgcgat ggcgcggagg agtgccaatt tcattgctcg tctctcctgt
    10501 gaagcgccga ttctgtttac tggtggcgtt agtcattgcc agacgtttgc ccggatgctg
    10561 gaatctcatc tgggaatgcc ggtaaatacc catcctgatg cgcaatttgc tggcgcaatt
    10621 ggcgcggcgg taattggtca acgagtgagg acacgccgat gaaagagttt ttatttttat
    10681 ttcactccac ggtcggcgtc atacaaaccc gcaaggcgtt gcaggcagcg ggcatgactt
    10741 ttcgcgtcag cgatattccc cgtgatttac gcggcggctg cgggttatgc atttggctga
    10801 cctgtccccc cggcgaggaa atacaatggg tgatccctgg gctgactgag tcaatttatt
    10861 gtcagcagga tggtgtctgg cgctgcattg cacattaccg ggtttctccg cgttaaggat
    10921 tcattaaggt cgctacgtat acttctcgat atcttattga tttgccttaa agggattacc
    10981 gtgacaaaaa gtatttcgtt aagtaaacgc atctccgtta ttgtgattct ttttgccatt
    11041 gtggcagtgt gtactttctt cgtgcaatct tgcgcccgta aaagcaatca tgcagcatca
    11101 tttcagaatt accacgccac cattgacggc aaagaaattg caggcataac gaataatatc
    11161 tcttcgctga cctggtcggc acaaagcaat actcttttta gcactatcaa caaacctgcc
    11221 gccattgttg aaatgaccac caatggtgat tttatccgca caattcctct ggattttgtt
    11281 aaggatctcg agacgatcga atacattggc gataatcagt ttgtcattag cgatgagcgc
    11341 gattacgcca tttatgtgat ttcactgacc ccaaactcgg aagtaaaaat cctcaaaaaa
    11401 ataaaaatcc cgctgcaaga ctccccaacc aattgcggtt ttgaagggtt ggcttattct
    11461 cgccaggatc acactttctg gttttttaaa gagaaaaatc cgattgaggt ctacaaagtt
    11521 aacgggctgt taagtagcaa tgaattacac atcagcaaag acgaggcact acaacggcaa
    11581 tttactttag atgatgtgtc cggcgcagaa tttaaccagc aaaaaaatac gcttctggtg
    11641 ctgtctcatg aatctcgcgc actccaggaa gtaaccctgg ttggcgaggt tatcggtgaa
    11701 atatcactaa ccaaaggtag tcgaggtttg tcgcataaca tcaaacaggc cgaagggatt
    11761 gcgatggatg cctctggtaa tatttatatc gttagcgaac caaatcgttt ttatcgcttt
    11821 acccctaaat catcgcatta acaggctggc atatttcacc tttttgttat taacttgtta
    11881 acaacaaaaa tgacatacaa ggttaaacat gccttcgtcc tcgcatcccg tagaacgctt
    11941 ttctttcagc accgcgcttt tcgggatgct ggttctgacc ttaggtatgg gattaggtcg
    12001 cttcctttat acgcctatgt tgcccgtcat gatggcggaa ggggcgtttt catttagcca
    12061 gctctcgtgg attgccagcg gcaactatgc ggggtatctg gctggcagtc tgctattttc
    12121 gtttggcgca tttcaccagc cgtcgcgcct gcgcccattt ctgttggttt ctgccctggc
    12181 gagcgggttg ttgatcctcg caatggcatg gttgccgcca tttattctgg tgttattgat
    12241 tcgcttcctg gcgggtgtcg ccagcgccgg tatgctgatt tttggttcga cgctgattat
    12301 gcagcacacg cgccatcctt ttgtgctggc agctttgttt tctggcgttg gcattggtat
    12361 tgcactgggc aacgaatatg ttctggcagg cctgcatttt gccttctcct cgcaaacgtt
    12421 atggcaaggc gccggggcgc tttctggcat gatgctgatt gcacttacgc ttttaatgcc
    12481 ctcgaaaaaa cacgccatcg caccaatgcc attggcaaaa acggagcaac agataatgag
    12541 ctggtggtta ctggctattc tgtacggcct tgcgggattt ggttatatca tcgtcgcgac
    12601 ttacctgccg ctcatggcga aagatgcagg ctcaccattg ttaaccgccc atctctggac
    12661 gttagtcggc ttatcaattg tgccaggctg ctttggctgg ctatgggcag caaaacgttg
    12721 gggggctttg ccttgcctga cggcgaattt gctggtgcag gctatcagtg tgctgcttac
    12781 tctcgccagc gactctcctc tcttgcttat cattagtagt atcggttttg gcggtacctt
    12841 tatgggcacg acatctctgg tgatgactat cgcccgccaa ctgagcgttc cgggaaatct
    12901 caatctttta ggctttgtga cgctcattta cggcattgga caaatcctcg gcccggcgct
    12961 gaccagtatg ctcggcaatg gaacatcggc gcttgccggc gccactctct gcggcgcggc
    13021 ggcgttgttt attgctgcgc ttatcagcac agtacaatta tttaagctgc aagtggtcac
    13081 ttcatgagga gtgtttccct taccactcaa gaaactcaac agcaaccgtt gatgtcagcc
    13141 agaccccgtt ttccgcttgc cagaaaggga tgcctcgttt cgccatttcc tgtgctttga
    13201 cggttaagat aaccggcgat ccgtgtcttg ctcccacttt gcgtgccgtt gcttcatcag
    13261 cagagagatg tacgtaatga cgctcccctg caatcagccc ctgttttttt atctcgtcaa
    13321 ggaaacggct tgccgtaccg tggtagagaa actgtggcgg tgttttttcg gcaaaggaaa
    13381 tggcgacctg cgaagtcgaa tgcccctgaa ccgcgcggat gcacctacca tcactggaat
    13441 aactaaaacg ctttttatcg ctggtggcaa ccacagtatc cagcaatgca cgggttaacc
    13501 gcttgcccgc tttttgggca caaagtatga gcttatcgat atccgcccat ccttcacggt
    13561 caagtacgat accaatcgcc tccggtttgt gtcggaggac aaaacttaaa aacttgctcg
    13621 tatccgccaa ctctttttcg ttgtactttg ccatcgcgtt ttcgtccctg gattttctgt
    13681 ttttatttac tgatgtcatt gtcactgggt ttatgaattt tctgcaactc aatcctgcgc
    13741 agagcctttt ctacacagcg taatttttcc gctatgcaaa aaacgcatag caagaaacat
    13801 acttgcattt tccccatacc cacatggctg ataaggtttt tcagctccag gaattttcct
    13861 ggctcaagca acacacaaac acaaaaaaac aatggggatt gttatgacac aacaagggga
    13921 tgctgtagcc ggggagctgg cgacagaaaa agtgggcatt aaagggtatc tggctttttt
    13981 tctcaccata atcttttttt ccggagtttt ttccgggaca gacagctggt ggcgcgtttt
    14041 tgatttttcc gttctgaacg gatcgtttgg gcaactgcct ggtgcgaatg gagccaccac
    14101 ctcatttcgc ggggcgggtg gcgcgggggc taaagatggc tttctctttg cgctggagct
    14161 ggcaccgtca gtcattctct ctctggggat tatttcaatt accgatggtc ttggtggatt
    14221 acgcgccgcg cagcagttaa tgacgcctgt gctaaaaccg ttgttgggca ttccagggat
    14281 ctgctcgctg gcgctgatcg ctaacctgca aaacactgat gccgccgccg gtatgaccaa
    14341 agagctggcg caggaagggg aaattaccga acgcgataaa gtgattttcg ccgcatatca
    14401 aaccagcggt agcgccatca tcaccaacta cttctcttcc ggcgtcgcgg tgttcgcctt
    14461 tctgggcacg tccgtaatcg tacctttagc cgtcatcctg gtgtttaaat ttgtcggagc
    14521 caacattttg cgcgtctggc ttaacttcga agaacgtcgc aacccaacgc aaggagcgca
    14581 agcatgacaa ctcaggtccg caaaaacgtc atggatatgt ttatcgatgg cgcgcgccgt
    14641 ggctttacta tcgccacgac caacctgctg ccaaacgtgg tgatggcgtt tgtcatcatt
    14701 caggcgctga aaattaccgg cctgctcgac tgggtggggc atatctgtga acccgtaatg
    14761 gcgctgtggg gattacccgg tgaagcggca accgtccttt tagccgcatt aatgagtatg
    14821 ggcggcgcag tcggtgttgc cgccagtctg gcgaccgccg gcgcattaac cggtcacgat
    14881 gtcaccgttt tactgcccgc gatgtattta atgggcaacc cggtgcaaaa tgtcggtcgc
    14941 tgcctgggga cagccgaagt gaatgccaaa tattacccgc atatcattac ggtctgcgtg
    15001 attaacgcat tgctgtcgat ctgggttatg cagcttattg tttaacaagg agttaccatg
    15061 attgattata ccgcagccgg ttttaccctg ctgcagggag cgcatttgta tgcgccggaa
    15121 gatcggggaa tttgcgatgt cctcgtcgct aacggcaaaa ttatcgccgt tgccagcaat
    15181 atcccttctg acattgtacc ggactgcacg gttgtcgatc tcagcgggca gatcctctgt
    15241 cctggtttta ttgatcaaca cgtccatttg attggtggtg gcggcgaagc aggtcccacg
    15301 acgcgcacgc cagaagtggc gctaagtcgc ctgacggaag cgggcgtcac gtcagtggtt
    15361 ggtctgctgg gcaccgactc tatctctcgc cacccggaat ccctgctcgc caagacccgt
    15421 gcgctcaatg aagaaggcat cagcgcctgg atgctgaccg gcgcttatca tgtcccttcc
    15481 cgcaccatta caggttccgt ggaaaaagac gtggcgatta tcgatcgtgt gattggcgtg
    15541 aaatgcgcca tctctgatca ccgttctgcc gcaccggacg tttatcacct ggctaatatg
    15601 gcggcggaat cccgcgttgg cggtttgctc ggcggtaaac ctggcgtcac cgtgttccac
    15661 atgggcgaca gcaaaaaggc gttacagcct gtctatgacc tgctggaaaa ctgcgatgtg
    15721 ccgatcagca agttgctgcc gacccacgtt aaccgcaacg ttccgttgtt tgagcaggcg
    15781 ctggagttcg cgcgcaaagg cggcactatc gatatcacca gcagcattga cgaaccggtc
    15841 gcccctgccg aaggtattgc ccgcgccgtt caggcgggta ttccgctggc acgcgtcacc
    15901 ctcagctccg acggcaacgg tagccagccg ttcttcgatg acgaagggaa tttaacccat
    15961 atcggtgttg ccggttttga aacgttgctg gaaaccgtgc aggtgctggt caaagactat
    16021 gatttcagta tcagcgatgc cctgcgcccg ctcaccagta gcgtagccgg tttccttaac
    16081 ctgaccggga aaggcgaaat tctgccaggc aatgatgctg acttgctggt catgacgcca
    16141 gaactgcgca ttgagcaggt atacgctcgc ggcaaactga tggtcaaaga cggcaaagcc
    16201 tgcgtgaatg gaacgtttga aacggcttaa acggttgtca gccgcccgaa tggacgtaga
    16261 atgcccgcaa gggcggctga cagagtaaaa cctaatggat gactgtggtg cggttttgca
    16321 taatattgaa accaaatggc tttatgattt tctgaccctg gaaaaatgcc gcaatttttc
    16381 ccaggcggca gtcagtcgca acgtctcgca accggcattc agccgccgca tccgtgcact
    16441 ggaacaggcg attggcgttg aattgtttaa ccgccaggtg acgccactgc aactctcgga
    16501 acaagggaaa atctttcatt cgcagatccg ccatctgttg caacagttag aaagtaacct
    16561 ggcagagctg cgtggcggca gcgattacgc gcaacgtaaa atcaagatag ccgctgcaca
    16621 ctctctttcc ctcggtctgt taccgtccat tatcagccag atgccgccgc tctttacctg
    16681 ggcaattgaa gccattgatg ttgatgaagc ggtcgataaa ctgcgtgaag ggcaaagtga
    16741 ctgtattttt tcctttcacg acgaagattt gctggaagcg ccgtttgacc acattcgctt
    16801 atttgaatct caattgttcc ctgtctgcgc cagtgacgaa cacggagaag cactttttaa
    16861 cctcgcgcag ccacactttc cgttactgaa ttacagccgc aactcctaca tggggcgatt
    16921 gattaatcgc accctgacgc gccacagtga gttaagtttc agcacctttt ttgtctcttc
    16981 gatgagcgag cttttaaagc aggttgccct cgacggctgt gggattgcct ggctgccgga
    17041 gtacgccatc caacaagaaa ttcgcagcgg gaagctcgtt gtactgaatc gggacgaact
    17101 ggtgatcccg attcaggcct atgcataccg aatgaatacc cgcatgaatc cagttgccga
    17161 acgcttctgg cgtgaactgc gcgagctgga gattgtgctt agctgacatt ctccagcatc
    17221 gcactacacc aaccgccatt atcccagacc agatcaaacg ttagcggttc accgtcgtgg
    17281 tacagcatta tctcaaggct gatcgccaga atgtggcagt acgcttcttt aaaattaacc
    17341 tgcaacgccg caatccgtgg ctcatgaaat aacagtgcag aacgcagctg acggcaatac
    17401 cagctctgga gatgtttgtc gctctggtta tgcacttgcc actctggcat gcctgaaaca
    17461 agtaatgacg gtaatgccga atgaggagaa ttaaaaagag tgaggagatc gctgcatacc
    17521 gatttacgca gcaatgacgt tttattgcct gaaatttcag gtaaaacagc ctgaagtgat
    17581 tggcgcatca ttttgcgcac tcctgacgtt tagcaacata tgcggcttgc cctgaacacc
    17641 gggcaccact gtattgatgc tcattttcca tagccattcc cttttgttca ttacatccat
    17701 gtcagtctga gacaggcctg aatcctgtca gcaaagtata aaaccttact tgcctatgaa
    17761 tatctactga cttttcagga aaaaagtcat gatttgataa aaatcatcaa actcaaaaaa
    17821 aaaccgctag tatattctga atattccgaa tagttacgta gat
//
LOCUS       DAPHDW010000089        17825 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.090, whole genome shotgun sequence.
ACCESSION   DAPHDW010000089 DAPHDW010000000
VERSION     DAPHDW010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17825
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.090"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            48..293
                     /locus_tag="RH057_002992"
                     /pseudo
     CDS             48..293
                     /locus_tag="RH057_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308281.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan endopeptidase"
     gene            271..663
                     /gene="fliS"
                     /locus_tag="RH057_002993"
     CDS             271..663
                     /gene="fliS"
                     /locus_tag="RH057_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004096838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV3129330.1"
                     /translation="MPKAEGRYSQYKEIDLAARTAAASPLELVLVLFSGLMDELERAK
                     SHIEGRRFEKKAQSINKCIDILNALTSSLEFETGGELVVNLSRLYDHCVYRLYEASGE
                     LSAEKIDEVMLILSNLREGWEGLSGKLG"
     gene            668..979
                     /locus_tag="RH057_002994"
     CDS             668..979
                     /locus_tag="RH057_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FliT"
                     /protein_id="HDV3129331.1"
                     /translation="MEKQRRQLFALVEAMNEALDKQRWRRLPALHQQLIRQFHDYAAA
                     ETDAAQLCAVKARLYGAFNALIKRREQRAEVLKARMEQHQRHQEGVLAYSIVNLLSEK
                     S"
     gene            976..2037
                     /locus_tag="RH057_002995"
     CDS             976..2037
                     /locus_tag="RH057_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HDV3129332.1"
                     /translation="MNPALLATLGTLAETASLKADILPPVSGENAPAFTLPKMAVAAV
                     AERVHSAKTSQQQATRPQENDPVAMQALMALLLPQPAAPHQDTPQPRNVATSPVIQQL
                     TKAVVQNAPQRPTQQQELTPLPPQLQELISQLPQAKPEQQARLATYASEDLHAIAPTQ
                     PRVSTQPARPKPELTRVTARPQVERKTEKVPDSEPVIARAVLQVKTPELVSDHQEIVA
                     KPATLSMDELGEKLTTLLKDQIHFQLNKQQQISTIRLDPPSLGKLEIAVQLDNGKLMV
                     HIGANQSEVCRALQQFSDDLRQHLTAQNFMEVNVQVSSEGQSQQQQQSGHQQEEVSAA
                     LQLDDAPQFQQNESVLIKV"
     gene            2045..2512
                     /locus_tag="RH057_002996"
     CDS             2045..2512
                     /locus_tag="RH057_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated FliL family
                     protein"
                     /protein_id="HDV3129333.1"
                     /translation="MKKIVVAGVVSAVLALVVGAGAGWGVWHHYAGKGKPTAAAQTET
                     VETLDESKSVFVTLPETIVTLHDNNGADHYLSAELVMVVASDKEAEKIKHQEPLYQSI
                     AVECLTEMKFEDLRGMKISAIRKLISDALKKDLQRRKMSAPYKDLLVKKVVFQ"
     gene            2532..3248
                     /locus_tag="RH057_002997"
     CDS             2532..3248
                     /locus_tag="RH057_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HDV3129334.1"
                     /translation="MLQDVFESEEFAAAPVLTPQQESHYLQAYLPLVRKVVRQLAPQC
                     NCIIDRQDMEQTALMGLLNAIRRYGLPDEGFAGYAVHRIRGAILDELRALDWRPRQLR
                     QKYHQMKDLIRETRKKLGHEPEWSELAVEGISHEDYLEYQQLEGAETLASLDELLNGE
                     GPGILLAGRELEDQFVTQQMLQQALSQLSEKEQLILSMYYQHEMNLKEIALVLGLTEA
                     RICQLNKQIAQKIRDFVYPN"
     gene            3261..4124
                     /gene="motA"
                     /locus_tag="RH057_002998"
     CDS             3261..4124
                     /gene="motA"
                     /locus_tag="RH057_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV3129335.1"
                     /translation="MQKILGLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGA
                     MIIGNPKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIE
                     QPEESTIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLT
                     PSRSLQRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYC
                     LMDPLANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANL
                     DAWVNAMLEQE"
     gene            4127..5050
                     /gene="lafU"
                     /locus_tag="RH057_002999"
     CDS             4127..5050
                     /gene="lafU"
                     /locus_tag="RH057_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV3129336.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            5121..6176
                     /gene="dinB"
                     /locus_tag="RH057_003000"
     CDS             5121..6176
                     /gene="dinB"
                     /locus_tag="RH057_003000"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV3129337.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            6173..6625
                     /locus_tag="RH057_003001"
     CDS             6173..6625
                     /locus_tag="RH057_003001"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3129338.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            6871..8010
                     /locus_tag="RH057_003002"
     CDS             6871..8010
                     /locus_tag="RH057_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDV3129339.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            8007..8621
                     /locus_tag="RH057_003003"
     CDS             8007..8621
                     /locus_tag="RH057_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDV3129340.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(8678..10135)
                     /gene="pepD"
                     /locus_tag="RH057_003004"
     CDS             complement(8678..10135)
                     /gene="pepD"
                     /locus_tag="RH057_003004"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV3129341.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            10396..10854
                     /gene="gpt"
                     /locus_tag="RH057_003005"
     CDS             10396..10854
                     /gene="gpt"
                     /locus_tag="RH057_003005"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV3129342.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            10946..12190
                     /gene="frsA"
                     /locus_tag="RH057_003006"
     CDS             10946..12190
                     /gene="frsA"
                     /locus_tag="RH057_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV3129343.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            12248..12649
                     /gene="crl"
                     /locus_tag="RH057_003007"
     CDS             12248..12649
                     /gene="crl"
                     /locus_tag="RH057_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV3129344.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(12688..13743)
                     /gene="phoE"
                     /locus_tag="RH057_003008"
     CDS             complement(12688..13743)
                     /gene="phoE"
                     /locus_tag="RH057_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV3129345.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
     gene            14031..15134
                     /gene="proB"
                     /locus_tag="RH057_003009"
     CDS             14031..15134
                     /gene="proB"
                     /locus_tag="RH057_003009"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HDV3129346.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSIYGIHVGQML
                     LTRADMEDRERFLNARDTLRALLDNNIVPVINENDAVATAEIKVGDNDNLSALAAILA
                     GADKLLLLTDQKGLYTADPRSNPQAELIKDVYGIDDALRAIAGDSVSGLGTGGMSTKL
                     QAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLENRKRWIFGAPPAG
                     EITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGRDIAHGVSRYNSDA
                     LRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            15146..16399
                     /gene="proA"
                     /locus_tag="RH057_003010"
     CDS             15146..16399
                     /gene="proA"
                     /locus_tag="RH057_003010"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HDV3129347.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLGEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGSVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESVEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALAQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
     gene            16514..16589
                     /locus_tag="RH057_003011"
     tRNA            16514..16589
                     /locus_tag="RH057_003011"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16547..16549,aa:Thr,seq:cgt)
     gene            16754..>17825
                     /locus_tag="RH057_003012"
     CDS             16754..>17825
                     /locus_tag="RH057_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3129348.1"
                     /translation="MPLNDMQIRRAKPEAKAYTLGDGQGLSLLVEPNGSKSWRFRYRY
                     AGKPKMISLGVYPTITLADARSRRDEARKLVAEGKNPSEVRKEQKLALQTESENAFEK
                     IAREWHQQKSTKWSAGYASDIMEAFKNDIFPYVGTRPVGEIKPLELLNVLRKIEKRGA
                     LEKMRKVRQRCSEVFRYAIATGRAEFNPAADLSSALNVHQSNHFPFLKANEIPDFLRA
                     LNGYTGSRLVLIATKLLMITGVRTIELRAALWSEFDLDNAIWEIPAERMKMRRSHLVP
                     LSTQALDLLNELKMMTGKYSYVFPGRNDPNKPMSEASINQVIKRIGYGGKLTGHGFRH
                     SLSTILHEKGYDSAWIEIQLAHI"
BASE COUNT         4449 a         4330 c         4786 g         4260 t
ORIGIN      
        1 gtttattaag tttgttcagg tacgggccgg gatacactgc aacatcaatg catgatcagc
       61 cattattgag tatgtccttg ccgtcgattc cctcttttgt gttgtcggga ttactgttgc
      121 tttgcttgcc gttttcttca tttgccagcg ccaccacatc acatatcact ttcagctacg
      181 ccgcccgcca gcggatgcaa aatcgtgcgc gtttattaaa acagtaccaa acccatctga
      241 aaaaacaggc cagctatatt gtggaaggca atgccgaaag cagaagggcg ctattcgcaa
      301 tataaagaga tcgatctcgc ggcgcgtact gcggcggcat cgcctctgga actggtgctg
      361 gtgctgtttt ctggcctgat ggacgaactg gaacgcgcca aaagccatat tgaagggcgg
      421 cgtttcgaga agaaagccca gagcatcaat aaatgcatcg acattctcaa tgcgctcacc
      481 agttcgctgg agtttgaaac tggcggcgag ctggtggtca acctttcgcg actctatgac
      541 cactgcgttt accgattgta tgaggccagc ggcgaactgt cggcagagaa aatcgatgaa
      601 gtgatgctga tcttaagcaa cctgcgcgaa ggctgggaag ggctttcggg caagctgggt
      661 taagacgatg gaaaaacaac gcaggcaact tttcgcactg gtcgaggcga tgaatgaagc
      721 tctcgacaag caacgctggc gacgccttcc agcactgcat cagcagttga tacgccagtt
      781 tcacgattac gcggcggcgg aaaccgacgc ggcgcagttg tgtgcggtga aggcgcgact
      841 gtacggcgcg tttaatgccc tgattaaacg gcgcgagcag cgcgcagaag tgttgaaggc
      901 gcgtatggaa cagcatcagc ggcatcagga aggtgtgctc gcctactcaa ttgttaattt
      961 actttcggag aagtcatgaa tccggcattg cttgccacgc tcggaacgct tgcggaaacc
     1021 gcttcgttga aggcggatat tttaccgccg gtgagcggtg aaaacgcgcc cgcgtttacc
     1081 ctgccgaaaa tggcggtcgc ggcggtggcg gagcgcgttc atagcgctaa aaccagtcag
     1141 caacaagcga cgcgcccgca ggagaacgat ccggtggcga tgcaggcgct aatggcgctg
     1201 ttacttccgc aacctgccgc gccgcatcag gacacgccgc agccgcgaaa cgtcgcaaca
     1261 tcgccggtta tccagcaatt gacgaaagcg gtggtgcaaa acgcgccgca acgcccgacg
     1321 caacagcagg aactcacgcc gttgccgccg cagttgcagg aactgatcag tcagttgccg
     1381 caggcgaagc ccgaacagca ggccagatta gcgacttacg ccagtgaaga tttacatgcc
     1441 attgcgccga cgcagccgcg cgtctcaaca cagccagctc gcccgaaacc tgaactaacc
     1501 cgtgtgaccg cgcgcccgca ggtcgagcgt aaaacggaaa aagtgccgga cagcgaacca
     1561 gttattgcgc gtgcggtgtt gcaggttaag acgccggagc tggtcagcga tcatcaggag
     1621 attgtcgcca aaccagccac gctttcgatg gatgaactgg gcgaaaaact gacgacgctg
     1681 ttgaaagatc agatccactt tcagctcaac aaacaacagc agatctccac catccgtctc
     1741 gatccaccgt cgcttggcaa gctcgagatc gccgtacaac tcgacaacgg caaactgatg
     1801 gtgcacatcg gcgcgaacca aagtgaagtt tgccgcgcgt tacagcagtt tagcgacgat
     1861 ctccgccagc atctgacggc gcaaaatttt atggaggtga acgtacaggt ttcctccgaa
     1921 gggcagtcgc agcaacaaca acagtcgggc catcagcagg aagaggtgag tgctgcctta
     1981 cagcttgatg atgcgcctca atttcaacag aacgaatccg ttttgatcaa agtgtaaagg
     2041 caatatgaag aaaatcgtgg tggcgggcgt ggtcagcgcc gttctggcgt tggtggtggg
     2101 ggccggagct ggatggggtg tctggcatca ttatgccgga aaaggaaaac cgactgccgc
     2161 cgcgcagacg gaaacggtgg aaaccctgga tgagagcaaa agcgtgtttg tgacgttgcc
     2221 ggaaaccatt gtcacgctgc atgacaataa cggcgcggac cattatttgt cggcggaatt
     2281 agtgatggtg gtcgccagtg acaaagaagc ggaaaaaata aaacatcagg agccgctgta
     2341 tcaaagtatt gcggttgaat gcctgacgga aatgaaattt gaagatttgc gtggcatgaa
     2401 aatatccgcc atccgcaaac tcatttctga cgcgttaaag aaagatcttc agcgtcgcaa
     2461 aatgagcgca ccgtataagg atttgctggt gaaaaaagtg gtcttccagt aatcaccagg
     2521 cggatataaa catgctacag gatgtattcg agtcagaaga atttgctgcc gcgccggtat
     2581 taaccccgca gcaggagagc cactacttgc aggcctattt accgttggtg cgtaaagtgg
     2641 tgcggcaact tgccccgcag tgtaattgca ttattgatcg tcaggatatg gaacaaacgg
     2701 cgttaatggg gctgcttaat gccatccgcc gctacgggct gccggacgaa ggttttgcgg
     2761 gctatgctgt gcaccgtatt cgcggcgcga tcctggatga gctgcgcgct ctcgactggc
     2821 gaccgcgcca gctgcgacag aaatatcatc agatgaaaga tctgattcgc gaaacgcgca
     2881 aaaaactggg gcacgaaccg gagtggtcag aactggccgt ggaagggatc tcgcatgaag
     2941 attatctcga atatcagcaa ctggaagggg cggaaacgct cgccagcctg gacgaactgc
     3001 tgaacggcga agggcctggc attctgcttg ctggcaggga gctggaagat cagtttgtca
     3061 cccagcaaat gttgcaacag gcgctttcac aattaagcga aaaagagcaa ctgatcctct
     3121 ccatgtatta ccagcatgag atgaatctca aagagattgc gctggtatta gggctaaccg
     3181 aggcccgaat ttgtcaactg aataaacaga tcgcgcagaa aattcgtgat tttgtctatc
     3241 cgaattaaag tgaagaaatc atgcaaaaaa tattaggttt actggtggtg ctcggctgcg
     3301 tatttggcgg ttattttgaa gcgggcgggc aattagtggc tatctggcaa ccggcggaaa
     3361 tgatcattat tttgggtgcc ggtttcgggg cgatgattat tggtaacccg aaacatgtgc
     3421 tgaaagagat tgcccatcaa attaaaggcg tgatcagtaa aaaacagctc gggccggagt
     3481 ttcagcgtca gctattaatg tgcctgtatg agttgctcga aatggtgcaa aacggtggtc
     3541 tgcgcatgct ggatcaacat attgagcaac cggaagagag caccattttc cagaaatatc
     3601 cgctggtgtt aacccagaag cggctggtga cctttattgc cgataacttt cgcctgatgg
     3661 cgatgggtaa aatcgacgcc catgaactgg aaggcattct cgatcaggaa ctggacactg
     3721 cggaagagtc gctgctcacg ccgtcgcgct cgctgcaacg taccgccgaa gcgatgccgg
     3781 gatttggtat ttgtgcagca gtgctgggga tcatcatcac catgcagtcg attgacggct
     3841 ctattgccct gatcggcctg aaagtggcgg cagcgctggt ggggacgttt ttaggcgtct
     3901 ttatctgcta ctgcctgatg gacccgctcg ccaacgccat ggaacaacag gcgcgggcag
     3961 aacattcttt gctggaatgc gtgcgcactg tgctggtggc gcaggcaggc ggcaaaccga
     4021 cgctgctggc ggtggatgcc gggcgtaagc tgctgcatct ggcgtccaaa ccgaccttcg
     4081 ccaacctcga cgcgtgggtg aatgcgatgc tggagcaaga ataagcatga gaaagacggg
     4141 caaccgccgc gagcgtgggg caaaaaccac catcgtccgg cggcagatca aaaagaatca
     4201 tgccgggcat catggcgggg cgtggaaggt ggcgttcgcc gactttactt tggcaatgat
     4261 ggcgctgttt atgaccctgt ggattgtcaa cagcgtcagt aaatccgagc gtgaaagcat
     4321 tatcgccgcg ctgcacgggc agtcgatttt taacggtggt gggctgtcgc cgttaaataa
     4381 aatcagcccg tctcatccgc caaaacctgc caccgtggcg gtgccggaag aaacggagaa
     4441 aaaagcgcgt gatgtgaatg agaaaacggc gctgctgaag aagaaatccg ccaccgagct
     4501 tggcgagctg gcaaccagta tcaccaccat tgcccgtgat gcgcatatgg aagcgaatct
     4561 ggagatggag attgttcccc agggattacg cgtgctgatt aaagacgacc agaatcgcaa
     4621 tatgttcgaa cgcggcagcg cgcagattat gccgttcttt aaaacgctgc tggtggagct
     4681 cgcgccagtt ttcgactcgc tcgataacaa aattattatt accgggcata ccgatgcgat
     4741 ggcttacaaa aacaatatct acaacaactg gaacctttcg ggtgaccgcg cgctttcggc
     4801 tcgtcgggtg ctggaagagg ccggaatgcc ggaagataaa gtgatgcagg taagcgcaat
     4861 ggcggaccag atgctgctgg attccaaaaa tccgcaaagc gcgggcaacc ggcgcattga
     4921 gattatggtg ctgaccaaaa gtgcgtccga tacgctgtat caatactttg gtcagcatgg
     4981 ggataaagtg gtgcagccgc tggtgcaaaa gctggataag cagcaggtgc tttcgcagcg
     5041 aacgcgttaa atgctgaatc tttacgcatt tctcaaaccc tgaaatcact gtatacttta
     5101 ccagtgttga gaggtgagta atgcgtaaaa tcattcatgt ggatatggac tgctttttcg
     5161 cagcggtgga gatgcgcgac aatcccgccc tgcgcgatat ccctattgct attggcggca
     5221 gccgcgaacg tcggggggtg atcagcaccg ccaattatcc cgcgcgtaaa tttggcgtac
     5281 gtagcgctat gccgacaggg atggcgctca aattatgccc gcatctcacc ttgcttccgg
     5341 ggcgctttga cgcctacaaa gaagcctcaa atcatatccg tgaaatcttc tcgcgctaca
     5401 cctcgcgtat tgaaccattg tcactggatg aggcttatct cgacgtcacc gatagcgtcc
     5461 attgccacgg ttctgcgacc ctcatcgccc aggaaatccg ccagacgatt ttcagcgagc
     5521 tgcaactgac agcgtctgcg ggcgtggcac cagtaaagtt tctcgccaaa atcgcctccg
     5581 acatgaataa acccaacggt cagtttgtga ttacgccggc agaagttccg gcatttttac
     5641 aaaccttacc gctggcaaaa atccccggcg tcggcaaagt ctcggcggca aaactggaag
     5701 cgatggggct gcggacctgc ggtgatgtac aaaagtgtga tctggtgata ctgcttaaac
     5761 gctttggcaa atttggccgc attttgtggg agcgtagtca ggggattgac gagcgcgacg
     5821 ttaacagcga acggttgcga aaatccgtcg gcgtggaacg cacgatggcg gaagatatcc
     5881 accactggtc tgaatgtgaa gcgattatcg agcggctgta tccggaactt gaacgccgtc
     5941 tggcaaaggt aaaacctgat ttactgattg cccgccaggg ggtgaaatta aagttcgacg
     6001 attttcagca aaccactcag gagcacgtct ggccgcggct gaataaagct gacttaatcg
     6061 ccaccgcgcg taaaacctgg gatgaacgcc gcggcgggcg cggtgtgcga ctggtggggc
     6121 tgcatgtgac gttgcttgac ccgcaaatgg aaagacaact ggtgctggga ttatgaataa
     6181 catacaaata agagactatc agcctggcga ttttcagcaa ctatgcgcta ttttcattag
     6241 agcggttacg atgactgcca gtcagcatta ttcaccacaa caaattgccg cctgggcgca
     6301 gattgacgaa tctcgctgga aggagaaact cgcgaaatca caagtgcggg ttgcagtcat
     6361 taatgcaaaa ctggtcggct ttattacctg cgttgaacac tatatcgata tgttatttgt
     6421 tgaccctgaa tacacccgcc gtggggttgc cagcgctttg ttaaaacctt tgattaagtc
     6481 tgaatccgaa cttacggtgg acgcaagcat aaccgcaaaa cccttttttg aacgttatgg
     6541 ttttcagaca gttaagcagc aacgcgttga atgtcgggga gagtggttta ttaattttta
     6601 tatgcgttat aaacagcaac attaaatcca gcttgcaatg aaaataacgc aagcctagta
     6661 tgtgcgcgtt ttcttattcc acaaactgca aggaggtaaa tcatgacaaa tcctttatca
     6721 atgactcttt gcagaccttt ccaggattaa ttcttttttt cttgccctgg attcgtctgc
     6781 catttcctga ttttttaatt gttactctca ataattcaag tcgcaacaca tgcaacttga
     6841 agtatgacga gtctatttac ggaatggtat atgggcaatt atattcgtcc cttatccgat
     6901 gcggtattta ccatcgcatc tgatgacctg tggatcgaga gtttagcgat ccaacaatta
     6961 cacaccacgg caaatttacc caacatgcag cgcgtagtgg ggatgccaga tttacatccc
     7021 ggacgcggct atccgattgg cgcagcgttc ttctctgttg gtcgttttta tccggcactg
     7081 gtcggcaatg atatcggctg cggtatggcg ctatggcaaa cagatattct cactcgcaaa
     7141 tacaacgccg ataagtttga aaagcgatta tcagatctgg atgacgttgc tgaagaaagc
     7201 tggctggagg aaaacctgcc gtcagcattt gcacagcatc cgtggcgcag ctcgcttggc
     7261 tctatcggtg gcggcaatca tttcgctgaa ctgcaacaag ttgatcaaat catcaacgct
     7321 gaactgtttg cactggcagg tctggatgca caacatctgc aactgttggt tcatagcggc
     7381 tcgcgggggg tggggcagtc tattttacag cggcatattg cctcgttctc gcatcatggt
     7441 ttgcctcaag gcagtgacga cgcgctacgg tatattgcgg aacatgacga tgcgctggcg
     7501 tttgcgtgta ttaatcgcca gatgatcgct ttgcgcataa tgcaacaagt taaggccacg
     7561 ggaagtccgg ttctggatgt ggcgcataac tttgttagcg cgtgtcaaat cggcgatcaa
     7621 cagggctggt tgcatcgtaa aggtgccacg ccggatgaca acggtctggt gattattccc
     7681 ggttcacgtg gtgattactc ctggattgta cagcctgttg cgaacgaaaa aacgctccac
     7741 tcgctggcgc atggggcagg gcgtaaatgg gggcgcaccg agtgtaaagg gcgtctggca
     7801 gcgaaataca cggcgacgca actctcgcgt actgaacttg gcagtcgggt aatttgtcgc
     7861 gataaacaac tcatctttga agaagcgcca caagcctata aatcggctga aagcgtggtg
     7921 caatgtctgg tgcaggccgg tttaattatt cctgtcgcgc ggctgcgtcc ggtgctaacg
     7981 ctcaaaaaca gtggagggaa aaaaggatga tcttgctaca actctcctca gcgcaggggc
     8041 cggaagaatg ttgtctcgca gtgaaaaagg cgctggacag gctaattaaa gaagctgccc
     8101 gacaagacgt cgcggtaacg gtgttggaaa cagaaacggg ccgctactct gacacgctgc
     8161 gttcagctct ggtttctctg gatggcgata acgcatgggc attaagcgaa agctggtgcg
     8221 gcactattca gtggatttgt ccgagtccgt atcggcctca tcatgggcgc aaaaactggt
     8281 ttctgggcat tgggcgtttt accgctgatg agcaggaaca atcggatgca atccgttatg
     8341 agacgttgcg ttcgtcgggg ccgggcggtc aacatgtcaa taaaaccgac tcggcggtac
     8401 gcgccacgca tttggcatcc ggtattagcg tgaaggttca gtcagagcgt agtcagcatg
     8461 ctaacaagcg gctggcacga ttgctgattg cctggaagct ggagcagcag caacaggaaa
     8521 atagcgcggt gctgaaatcg cagcggcgaa tgttccatca ccagattgaa cgtggcaacc
     8581 cgagacggac atttacaggg atggctttta tcgaaggata atgaaggatg aaactgccgg
     8641 aaggcgatta aacgccatcc ggcagcaaat caaataatta cttcgccgga atttctttca
     8701 gcagttcagt cagcagtgtc cagtaatgac ctacgctttc gatgtgaact tgctcatccg
     8761 gagagtgtgg accggtgata gttggcccga tagaaaccat gtccatttcc ggatacggtt
     8821 ttttgaacag accacattcc aggcccgcgt ggataatctg gatgttcggt gttttgttga
     8881 acagacgctg ataggtttca cgtaccagat gcatcaccgg agaattagcg tccggctgcc
     8941 agccaggata tgcgcctttc gcttcggttt tcgcgccagc tagtttaccc agcgaatcca
     9001 gcatgctcac cacgtagtct ttaccgctgt cgatcagtga acggatcagg cagtgaattt
     9061 ctacgttatt gtcagtcatg gtcaccacac cgacgttcag ggaggtttcg accacacctt
     9121 ttgccacgtc ggaattgcgg atcacaccgt tcggggtggc gttcagcaga cgaataaagg
     9181 tatcgcgaga tttcgcaatc agggcagctt tatcgttcgc tacagagtcc agcaacaagg
     9241 ccagattttt ctctttctct gccagctcgt ttttcaggat ctcctgatag gtattcacca
     9301 gagatttcag gacgtcgact ttatcagctg cgacagcaat ggtcgcaaag gcttcacgcg
     9361 ggatggcgtt acgcagtgtg ccgccgttga aatcgataag gcgcagatcc agttcttccg
     9421 catgacccgc caggaagcgc accagtagtt tgttggcatt acccaggcca acgtggattt
     9481 caccgccgga gtgaccgcct ttcagacctt ttaaggttaa cttgaaggtt tcaaaaccag
     9541 ctggaaccgc ttcacgatct aaatgcaggt tggaggtgaa gtcgataccc cccgcacaac
     9601 ccatgtagat ttcaccttct tcttcggagt cggtgttaat cagaatatca gcctgcaacc
     9661 agttgctctg taagccgaac gcaccgtcca taccggcttc ttcggtcatg gtcagcagca
     9721 cttccagcgg gccgtgaacc acgttttcgt cagccagaac cgccagcgca gacgccatac
     9781 caatgccgtt atctgcaccc agcgtggtgc cgcgcgcttt aacccattcg ccatcaatat
     9841 aaggctggat aggatctttc gtgaagtcat gcacggtgtc gttatttttc tgcggcacca
     9901 tatcgaggtg ggcctgtaag acgaccggtt tacgattttc catacctgcg gtagcaggtt
     9961 tacgaatcag gatattacct acctgatcgc gttcgacatg gaaacctttc tcttttgccc
    10021 aaccaacaat gtattcagcg agttgctctt catgatagga cgggtgagga atagaacaga
    10081 ttttggcaaa aatatcccac agcggctgtg gagataattg agacagttca gacacgttaa
    10141 gtctccttgt cgatcacccg caaaacagta ttgcaggtca cagggttagc agaaaatgtt
    10201 gtcaacacaa gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg
    10261 agaggcagtg cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat
    10321 ctctataatc tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag
    10381 gctgggacac ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat
    10441 gcacgtaaac tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta
    10501 agccgtggcg gtctggtacc gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc
    10561 gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa agtgctaaaa
    10621 cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga taccggtggt
    10681 actgcggttg cgattcgtga aatgtatcca aaagcacatt ttgtgactat cttcgcaaaa
    10741 ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga tacctggatc
    10801 gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt
    10861 caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac aatagtttcc
    10921 agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa ccctgttcaa
    10981 accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc acggcgcaca
    11041 accgcctgtg cagtcggctc ttgatggtaa aaccatccct cactggtatc gcatgattaa
    11101 ccgtctgatg tggatctggc gcggcattga cccacgtgaa atcctcgacg tccaggcacg
    11161 tattgtgatg agcgatgctg aacgtaccga tgatgattta tacgataccg tgattggcta
    11221 ccgtggtggc aactggattt atgagtgggc cacccaggcg atggtgtggc aacaaaaagc
    11281 ctgtgcggaa gaagatccgc aactcagtgg tcgacactgg ctgcatgcgg ctacgttgta
    11341 caacattgcc gcctatcctc atctgaaagg agatgacctg gccgagcaag cgcaggcttt
    11401 atcaaaccgc gcctatgaag aggccgccca gcgtctgccg ggcacgatgc ggcagatgga
    11461 gtttaccgta cccggcggtg cgcccatcac cggctttttg catatgccga aaggcgatgg
    11521 cccgttcccg acagtattaa tgtgtggtgg tctggatgcg atgcagacgg actattacag
    11581 cctgtatgaa cgttattttg cgccgcgcgg cattgcgatg ctgactattg atatgccgtc
    11641 ggtgggcttt tcttcaaaat ggaagctcac ccaggactcc agcctgttgc atcagcacgt
    11701 cttaaaggcg ctgcctaacg taccgtgggt ggatcacact cgcgtcgcgg cctttggttt
    11761 ccgtttcggc gctaacgttg ccgtgcgtct ggcatacctt gaatcgccgc gtctgaaagc
    11821 ggttgcctgt cttggtccgg tagttcatac cctgttgagt gattttaagt gccagcaaca
    11881 ggtgccggaa atgtatcttg acgttctggc gagtcgtttg gggatgcatg atgcttccga
    11941 tgacgcgttg cgcgtggagc tgaatcgcta ttcattaaaa gtgcaaggat tgctgggacg
    12001 tcgctgccca acgccaatgt tatcaggcta ctggaagaac gatccgttca gcccggaaga
    12061 ggactcacgc ttaatcacct catcatctgc tgacggtaaa ttattagaga tcccatttaa
    12121 cccggtgtat cggaattttg acaaaggtct tcaggaaatc accggctgga tcgaaaaacg
    12181 cttgtgttaa aaatttgcta aattttgcca atttggtaaa acagttgcat cacaacagga
    12241 gatagcaatg acgttaccga gtggacaccc gaagagcaga ttgatcaaaa aatttaccgc
    12301 actaggcccg tatattcgtg aaggtaagtg cgaagataat cgattctttt tcgattgtct
    12361 ggctgtatgc gtcaacgtga aaccggcacc ggaagtgcgt gaattctggg gctggtggat
    12421 ggagctggaa gcgcaggaat cccgttttac ctacagttac cagtttggtc tgttcgataa
    12481 agcaggcgac tggaagagtg ttccggtaaa agacactgaa gtggttgaac gactggagca
    12541 caccctgcgt gagtttcacg agaagctgcg tgaactgctg acgacgctga atctgaagct
    12601 ggaaccggcg gatgattttc gtgacgagcc ggtgaagtta acggcgtgag tgaaatgtgc
    12661 cggatgcatc acatccggca ataatcatta aaactgatag gtcatgccaa ccgcgacaat
    12721 atcatcatta ttaatattca atttgttatc gctatccagt tggttgattt tataatcaac
    12781 aaacgctgac atatttttgt tgaaataata tgtagccccg acatcgatat aattgaccag
    12841 atcttcatca ccgatacctt caatatcttt ccctttcgat aagacataac ccagcgatgg
    12901 acgcagacca aagtcaaact ggtattgagc gaccgcttca aagttctgtg tcttattggc
    12961 aaagccgcca gttattggcg tcattttgcg tgtttcagaa taaaaagttg ccagataaat
    13021 attattggca tcgtatttca gacctgttgc ccatgcttct gcacgcttgc ctgtgccacg
    13081 gctttgcagg ttctgctcgt tggtgcgatc tgagttggta taggccccac taatggcgaa
    13141 atcgctgccg ccaaagtcat atgtcaatga cgtgccgaag ccatcgccgt tttgcttttt
    13201 aacgtcgcgg ttttcgtttt tcccttgata ttgcagggtt aagttcaggc catcgataac
    13261 gccgaagaag tcggtgttcc gatacgtcgc cagaccgctg gcgcgtttgg tcataaagtt
    13321 gtcggtctgc gccgaggagt cgccaccaaa ttccgggaac atatcggtcc aggcttccac
    13381 gtcatacaac gcgcccaggt tacggccata gtcgaaagaa cccaaatctt tatacttcaa
    13441 tccggcaaaa gcgagacgcg ttttttgctg tgcagtatca ctctccgctt tatttccggc
    13501 aaactccgct tcccaacggc catagccagt cagttgatcg ttaatttgtg tttcgccttt
    13561 aaaaccaaaa cggatataac tctggtcgcc atctttactg tcgttatcac tcatataatg
    13621 catggcttta actttgccat agacatccag tttattaccg tctttgttat atatttctgc
    13681 ggcctgtacg gatgcagatg ccacaatgcc catcaccact aatgccagag tgctcttttt
    13741 cattttcatt cctgatttta attaacgcgc gaatattcag cgggagagcc ccgttgaaaa
    13801 caggaaagtt tttaacctga gattgttaaa gatatattac agattaataa tattcttaaa
    13861 atgtggtaat ttattaaatc tgtaataaaa gcgtaaacaa ctgccgctag gcttgctgat
    13921 cccgcgcaac aaaacgccat gctttgcttg cagatggttg gcaaccgacg acagtcctgc
    13981 taaaacgttc gtttgatatc atttttccta aaattgaatg gcagagaatc atgagtgaca
    14041 gccagacgct ggtggtaaaa ctcggcacca gtgtgctaac aggcggatcg cgccgtctga
    14101 accgtgccca tatcgttgaa cttgttcgcc agtgcgcgca gttacatgcc gccgggcatc
    14161 ggattgttat tgtgacgtcg ggcgcgatcg ccgccggacg tgagcacctg gggtacccgg
    14221 aactgccagc gactatcgcc tcgaaacaac tgctggcggc ggtagggcag agtcgactga
    14281 ttcaactgtg ggaacagctg ttttcgattt atggcattca cgtcgggcag atgctgctga
    14341 cccgtgctga tatggaagac cgtgaacgct tcctgaacgc ccgcgacacc ctgcgtgcgt
    14401 tgctcgataa caatatcgtt ccggtaatca atgagaacga tgctgtcgct acggcagaga
    14461 ttaaggtcgg cgataacgat aacctttctg cgctggcggc gattctggcg ggtgccgata
    14521 aactgttgct gctgaccgat caaaaaggtt tgtataccgc tgatccgcgc agcaatccgc
    14581 aggcagaact gattaaagat gtttacggca ttgatgacgc actgcgcgcg attgccggtg
    14641 acagcgtttc aggcctcgga actggcggca tgagtaccaa attgcaggcc gctgacgtgg
    14701 cttgccgtgc gggtatcgac accattattg ccgcgggcag caagccgggc gttattggtg
    14761 atgtgatgga aggcatttcc gtcggtacgc tgttccatgc ccaggcgact ccgcttgaaa
    14821 accgtaaacg ctggattttc ggtgcgccgc cggcgggtga aatcacggta gatgaagggg
    14881 caactgccgc cattcttgaa cgcggcagct ccctgttgcc gaaaggcatt aaaagcgtga
    14941 ctggcaattt ctcgcgtggt gaagtcatcc gcatttgcaa cctcgaaggt cgcgatatcg
    15001 cccacggcgt cagtcgttac aacagcgatg cattacgccg tattgccggg caccactcgc
    15061 aagaaattga tgcaatactg ggatatgaat acggcccggt tgccgttcac cgtgatgaca
    15121 tgattacccg ttaaggagca ggctgatgct ggaacaaatg ggcattgccg cgaagcaagc
    15181 ctcgtataaa ttagcgcaac tctccagccg cgaaaaaaat cgcgtgctgg aaaaaatcgc
    15241 cgatgaactg gaagcacaaa gcgaaatcat cctcaacgct aacgcccagg atgttgctga
    15301 cgcgcgtgcc aatggccttg gcgaagcgat gcttgaccgt ctggcactga cgcccgcacg
    15361 gctgaaaggc attgccgatg atgtgcgcca ggtgtgtaac ctcgccgatc cggtggggca
    15421 ggtaatcgat ggcagcgtac tggacagcgg cctgcgtctt gagcgtcgtc gcgtaccgct
    15481 gggggttatt ggcgtgattt atgaagcgcg cccgaacgtg acggttgatg tcgcttcgct
    15541 gtgcctgaaa accggtaatg cggtgatcct gcgcggtggc aaagaaacgt gtcgcactaa
    15601 cgctgcaacg gtggcggtga ttcaggacgc cctgaaatcc tgcggcttac cggcgggtgc
    15661 cgtgcaggcg attgataatc ctgaccgtgc gctggtcagt gaaatgctgc gtatggataa
    15721 atacatcgac atgctgatcc cgcgtggtgg cgctggtttg cataaactgt gccgtgaaca
    15781 gtcgacaatc ccggtgatca caggtggtat aggcgtatgc catatttacg ttgatgaaag
    15841 tgtagagatc gctgaagcat taaaagtgat cgtcaacgcg aaaactcagc gtccgagcac
    15901 atgtaatacg gttgaaacgt tgctggtgaa taaaaacatc gccgatagct tcctgcccgc
    15961 attaagcaaa caaatggcgg aaagcggcgt gacattacac gcagatgcag ctgcactggc
    16021 gcagttgcag gcaggccctg cgaaggtggt tgctgttaaa gccgaagagt atgacgatga
    16081 gtttctgtca ttagatttga acgtcaaaat cgtcagcgat cttgacgatg ccatcgccca
    16141 tattcgtgaa cacggcacac aacactccga tgcgatcctg acccgcgata tgcgcaacgc
    16201 ccagcgtttt gttaacgaag tggattcgtc cgctgtttac gttaacgcct ctacgcgttt
    16261 taccgacggc ggccagtttg gtctgggtgc ggaagtggcg gtaagcacac aaaaactcca
    16321 cgcgcgtggc ccaatggggc tggaagcact gaccacttac aagtggatcg gcattggtga
    16381 ttacaccatt cgtgcgtaaa taaaaccggg tgatgcaaaa gtagccattt gattcacaag
    16441 gccattgacg catcgcccgg ttagttttaa ccttgtccac cgtgattcac gttcgtgaac
    16501 atgtcctttc agggccgata tagctcagtt ggtagagcag cgcattcgta atgcgaaggt
    16561 cgtaggttcg actcctatta tcggcaccat ctccacttct tcaaacatcc gtatttatcc
    16621 ataaccgcac tgttttataa aagatttttt gtttttactg ttcgtattag tccataactt
    16681 tccagtagaa tccagtacta aatgtgtata ggattatgta tatgttcctg ttcgattttg
    16741 gaattctata cacatgcccc taaatgatat gcagattcgc cgtgctaaac ctgaagctaa
    16801 agcctataca cttggggatg ggcaagggtt gtctttactt gtagagccaa atggaagtaa
    16861 aagctggcga tttcgttatc gctatgccgg taaacccaaa atgatctcgc ttggtgttta
    16921 cccaacgatc actcttgctg atgctcgttc ccgtcgtgat gaagctcgaa aacttgtggc
    16981 agaaggaaag aaccctagtg aggttcgaaa agagcaaaag ctggctctgc aaacagagtc
    17041 agagaacgcc ttcgaaaaga tagccagaga gtggcatcaa cagaagtcta ccaaatggtc
    17101 ggcgggatat gcatcagaca tcatggaagc gtttaagaac gacatttttc cttatgtggg
    17161 aacaaggcca gtgggagaga ttaaaccgct agaactgctt aatgtgctgc gtaaaatcga
    17221 aaagcgcggt gcattagaaa aaatgcgcaa agttcggcag cgatgctcag aagttttccg
    17281 ctatgccatt gctactggaa gggctgagtt taaccctgct gcggatcttt caagcgccct
    17341 caatgtacac caatcaaatc atttcccgtt cttaaaggct aatgagatac ctgattttct
    17401 tcgcgcctta aacggatata ccggaagtcg gcttgtcctg attgccacga aattgctcat
    17461 gattacaggt gttagaacca tcgaattacg tgcggcatta tggtcagaat ttgatttaga
    17521 taacgctatt tgggaaattc ctgctgaaag gatgaaaatg cgcagatcac accttgtgcc
    17581 tttgtcgact caagcgttag atttgctaaa tgaactcaag atgatgacag ggaagtatag
    17641 ttatgttttt ccggggcgga acgatccgaa caagcctatg agtgaggcga gtattaacca
    17701 agttatcaag cgtattggtt atggtggaaa acttactggt catggatttc gacattcctt
    17761 atctactatc ctccacgaaa aaggatatga ttcggcttgg atagaaatac agcttgctca
    17821 tatag
//
LOCUS       DAPHDW010000090        17678 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.091, whole genome shotgun sequence.
ACCESSION   DAPHDW010000090 DAPHDW010000000
VERSION     DAPHDW010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17678)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17678)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17678
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.091"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            37..1977
                     /gene="csrD"
                     /locus_tag="RH057_003013"
     CDS             37..1977
                     /gene="csrD"
                     /locus_tag="RH057_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HDV3129349.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSTLRPQITELMMSADIVRVDLLHGDKQVYTLARNGSYRPVGSSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIIVMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIAGQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTQVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            2282..3325
                     /gene="mreB"
                     /locus_tag="RH057_003014"
     CDS             2282..3325
                     /gene="mreB"
                     /locus_tag="RH057_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HDV3129350.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            3391..4494
                     /gene="mreC"
                     /locus_tag="RH057_003015"
     CDS             3391..4494
                     /gene="mreC"
                     /locus_tag="RH057_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HDV3129351.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGVSQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPTTGNAATA
                     PAAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            4494..4982
                     /gene="mreD"
                     /locus_tag="RH057_003016"
     CDS             4494..4982
                     /gene="mreD"
                     /locus_tag="RH057_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HDV3129352.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSTLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            4991..5584
                     /gene="yhdE"
                     /locus_tag="RH057_003017"
     CDS             4991..5584
                     /gene="yhdE"
                     /locus_tag="RH057_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HDV3129353.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADTIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            5574..7043
                     /gene="rng"
                     /locus_tag="RH057_003018"
     CDS             5574..7043
                     /gene="rng"
                     /locus_tag="RH057_003018"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HDV3129354.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            7111..10911
                     /gene="yhdP"
                     /locus_tag="RH057_003019"
     CDS             7111..10911
                     /gene="yhdP"
                     /locus_tag="RH057_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HDV3129355.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLDAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGGNDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSTLKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTSVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVEMNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPENERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            11067..12512
                     /gene="tldD"
                     /locus_tag="RH057_003020"
     CDS             11067..12512
                     /gene="tldD"
                     /locus_tag="RH057_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HDV3129356.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(12640..13569)
                     /gene="aaeR"
                     /locus_tag="RH057_003021"
     CDS             complement(12640..13569)
                     /gene="aaeR"
                     /locus_tag="RH057_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HDV3129357.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSVNLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            13752..13955
                     /locus_tag="RH057_003022"
     CDS             13752..13955
                     /locus_tag="RH057_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HDV3129358.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            13963..14895
                     /gene="aaeA"
                     /locus_tag="RH057_003023"
     CDS             13963..14895
                     /gene="aaeA"
                     /locus_tag="RH057_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HDV3129359.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQVLFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSNLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            14901..16868
                     /gene="aaeB"
                     /locus_tag="RH057_003024"
     CDS             14901..16868
                     /gene="aaeB"
                     /locus_tag="RH057_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HDV3129360.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            16960..17232
                     /locus_tag="RH057_003025"
     CDS             16960..17232
                     /locus_tag="RH057_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HDV3129361.1"
                     /translation="MNIYTFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(17288..17551)
                     /gene="yhcN"
                     /locus_tag="RH057_003026"
     CDS             complement(17288..17551)
                     /gene="yhcN"
                     /locus_tag="RH057_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HDV3129362.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDMREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
BASE COUNT         4125 a         4328 c         4913 g         4312 t
ORIGIN      
        1 tatgcccgct tcctcactat cggagttaac acaaggatga gattaacgac gaaattttcg
       61 gcctttgtta cgctgctcac cgggttaaca atttttgtga ctttgctggg ctgttcgcta
      121 agtttctaca acgcaattca gtataagttt agtcatcgcg ttcaggcggt ggcgacggcg
      181 attgataccc accttgtgtc gaatgacttc agcacattaa ggccacaaat taccgaatta
      241 atgatgtcgg cagatatcgt tcgtgtagac ctgctccatg gtgataaaca ggtttatacc
      301 ctggccagaa atggtagtta tcgtccggtt ggctccagcg atctgtttcg cgaactgagt
      361 gttccgttga taaagcatcc ggggatgtcg ctgcgtctgg tttatcagga tccgatgggc
      421 aactatttcc attctttaat gactaccgcg ccgctcacgg gggcgattgg ctttatcatt
      481 gttatgctct ttctggcggt acgctggtta caacggcaac ttgccgggca agaattgctg
      541 gaaacccggg ctactcgtat cttaaacggt gagcgtggct ctaatgtgtt gggaaccatc
      601 tatgaatggc cgcccagaac cagcagtgcg ctagatacgc tgcttcgtga aattcagaac
      661 gcacgcgaac aacacagccg tcttgatacg ctgatccgct cttatgccgc ccaggacgtg
      721 aaaaccggcc tcaataaccg actctttttc gataatcagt tagcaacgtt actggaagat
      781 caggagaaag taggtaccca cgggatcgtg atgatgattc gtctgccgga tttcaatatg
      841 ttgagcgata cttgggggca cagccaggtt gaagaacagt tcttcactct gacgaatctg
      901 ctgtcgacat ttatgatgcg ctaccctggc gcactgctgg cgcgttacca ccgcagtgat
      961 tttgctgcgc tgttaccgca ccggacgtta aaagaggcag agagcatcgc cggtcagtta
     1021 atcaaagccg tcgatacctt gccgaacaat aaaatgctcg atcgcgacga tatgatccac
     1081 attggtatct gcgcctggcg tagtggtcag gataccgagc aggtaatgga acatgcagag
     1141 tctgccacgc gtaatgcggg attgcagggc ggcaatagct gggctattta cgatgactcg
     1201 ttgcctgaaa aaggacgcgg taatgttcgc tggcgtacgc ttatcgagca aatgctcagt
     1261 cgcggcggcc cgcgccttta tcaaaaaccg gcggttactc gcgaaggtca ggttcatcat
     1321 cgcgaactca tgtgccgcat cttcgatggt aatgaagagg ttagctcggc ggagtatatg
     1381 ccgatggtct tgcagtttgg cttatcggaa gagtatgacc gtctgcaaat cagccgtctt
     1441 attccactat tgcgttactg gccagaggaa aatctggcga ttcaggttac cgttgagtcg
     1501 ctgattcgcc cgcgttttca gcgttggctg cgcgatacgt taatgcaatg tgaaaaatca
     1561 caacgaaaac gcataattat tgaacttgca gaggccgatg taggtcaaca tatcagtcgt
     1621 ttacaacctg ttattcgttt agtgaatgct ttaggggtac gggtagccgt caaccaggct
     1681 ggtttgacgc tggtaagcac cagttggatc aaagaactta atgttgagtt actcaagctc
     1741 catccggggc tggtcagaaa cattgagaag cgaacggaga accaactgct ggttcaaagc
     1801 ctggtggaag cctgctccgg gaccagcacc caagtttacg ccaccggtgt gcgttcgcga
     1861 agcgagtggc agaccctgat tcagcgcggt gttacaggcg ggcaagggga ttttttcgcg
     1921 tcctcacagc cacttgatac taacgtgaaa aaatattcac aaagatactc ggtttaacct
     1981 gccgtttaat ctgttttcac gtagaataac gcgcgctgcg tctcatggga gtgtgcttgt
     2041 ctgctcgcca gattgttgca gcacatatgc agatgaatga ccttacgcgg ttgcaaacag
     2101 gcgaggaatg ctgctgatgc attaagcctt tctggactca ggcagagatt tgtaacaaag
     2161 gaaacgaact gcactaattt tcaccgtagc agatgatttt tgcgccttgt cgctgctgcg
     2221 tgtggttggt aaagtaagcg gattttcttt tccgccccag ctttcaggat tatcccttag
     2281 tatgttgaaa aaatttcgtg gcatgttttc caatgacttg tccattgacc tgggtactgc
     2341 gaataccctc atttatgtaa aaggacaagg catcgtattg aatgagcctt ccgtggtggc
     2401 cattcgtcag gatcgtgccg gttcaccgaa aagcgtagct gcagtaggtc atgacgcgaa
     2461 gcagatgctg ggccgtacgc cgggcaatat tgctgccatt cgcccaatga aagacggcgt
     2521 tatcgccgac ttcttcgtga ctgaaaaaat gctccagcac ttcatcaaac aagtgcacag
     2581 caacagcttt atgcgtccaa gcccgcgcgt tctggtttgt gtaccggttg gcgcgaccca
     2641 ggttgaacgc cgcgcaattc gtgaatccgc gcagggcgct ggtgcccgtg aagtcttcct
     2701 gattgaagaa ccgatggctg ccgcaattgg tgctggcctg ccggtttctg aagcgaccgg
     2761 ttctatggtg gttgatatcg gtggtggtac cactgaagtt gctgttatct ccttgaacgg
     2821 cgtggtttac tcctcttccg tgcgcattgg tggtgatcgt ttcgacgaag ctatcatcaa
     2881 ctatgtgcgt cgtaattacg gttctctgat cggtgaagcc accgcagaac gtatcaagca
     2941 cgaaatcggt tcggcttacc cgggcgatga agtccgtgaa atcgaagttc gtggccgtaa
     3001 cctggcagaa ggtgttccac gcggttttac cctgaactcc aacgaaatcc tcgaagcact
     3061 gcaggaaccg ctgaccggta ttgtgagcgc ggtaatggtt gcactggaac agtgcccgcc
     3121 ggaactggct tccgacatct ccgagcgcgg catggtgctc accggtggtg gcgcactgct
     3181 gcgtaacctt gaccgtttgt taatggaaga aaccggcatt ccagtcgttg ttgctgaaga
     3241 cccgctgacc tgtgtggcgc gcggtggcgg caaagcgctg gaaatgatcg acatgcacgg
     3301 cggcgacctg ttcagcgaag agtaatcgga tgcaggcagg ggaaatgtct gtttaccctg
     3361 cctggtctga tacgagaata cgcataactt atgaagccaa tttttagccg tggcccgtcg
     3421 ctacagattc gccttattct ggcggtgctg gtggcgctcg gcattattat tgccgacagc
     3481 cgcctgggga cgttcagtca aatccgtact tatatggata ccgccgtcag tcctttctac
     3541 tttgtttcca atgctcctcg tgaattgctg gatggcgtat cgcagacgct ggcctcgcgt
     3601 gaccaactag aacttgaaaa ccgggcgtta cgtcaggaac tgttgctgaa aaacagtgaa
     3661 ctgctgatgc ttggacaata caaacaggag aacgcgcgtc tgcgcgagct gctgggttcc
     3721 ccgctgcgtc aggatgagca gaaaatggtg actcaggtta tctccacggt taacgatcct
     3781 tatagcgatc aagttgttat cgataaaggt agcgttaatg gcgtttatga aggccagccg
     3841 gtcatcagcg acaaaggtgt tgttggtcag gtggtggccg tcgctaaact gaccagtcgc
     3901 gtgctgctga tttgtgatgc gacccacgcg ctgccaatcc aggtgctgcg caacgatatc
     3961 cgcgtaattg cagccggtaa cggttgtacg gatgatttgc agcttgagca tctgccagcg
     4021 aatacggata ttcgtgttgg tgatgtgctg gtgacctccg gtctgggcgg tcgtttcccg
     4081 gaaggctatc cggtcgcggt tgtctcttcc gtaaaactcg atacccagcg cgcttatact
     4141 gtgattcagg cgcgtccgac tgcaggactg caacgtttgc gttatctgct gctgctgtgg
     4201 ggcgcggatc gtaacggcgc taacccgatg acgccggaag aggtgcatcg tgttgctaat
     4261 gaacgtctga tgcagatgat gccgcaggta ttgccttcgc cagacgcgat ggggccaaag
     4321 ttacctgaac cggcaacggg gatcgctcag ccgactccgc agcaaccgac gacaggaaat
     4381 gcagctactg cgcctgctgc gcccacacag cctgctgcta atcgctctcc acaaagggct
     4441 acgccgccgc aaagtggtgc tcaaccgcct gcgcgtgcgc cgggagggca atagtggcga
     4501 gctatcgtag ccagggacgt tgggtaatct ggctctcttt cctcattgcg ctgttgctgc
     4561 aaatcatgcc ctggccggat aacctgattg ttttccggcc aaactgggtg ttactcatct
     4621 tgttgtattg gatcctggcc ttgcctcatc gcgtaaatgt gggcacaggt tttgtgatgg
     4681 gtgccatact ggatctgatc agcggctcga cgcttggcgt acgcgtattg gcgatgagca
     4741 tcattgctta cctggtggcg ctgaaatacc agcttttccg caacctcgca ttatggcagc
     4801 aggcgctggt cgtcatgttg ctttcgctgg tggtggatat tattgttttc tgggcagagt
     4861 ttttagtgat taacgtctct ttcagaccgg aagtgttctg gagtagtgta gtcaatgggg
     4921 tgctctggcc gtggattttc ttgctgatgc gtaaagtccg tcagcagttt gcagtgcaat
     4981 aaaggtttct atgacttctc tgtatttagc ttccggttct ccgcgtcgtc aggagttact
     5041 tgcgcaactt ggcgtgacct ttgaacgtat tgttacaggc attgaggagc agcgtcagcc
     5101 gcaggagagc gcgcagcagt atgttgtgcg tctggcgcgc gagaaagcac aggcgggtgt
     5161 cgcgcaaacg gcgcaggatc tcccggtgct gggtgcggat actatcgtta tcctgaatgg
     5221 agaagtgctg gagaaaccgc gcgacgcaga gcatgcggcg cagatgttgc gcaaattatc
     5281 gggtcagacc catcaggtga tgacggcagt ggcgttggcc gacagccagc acattctcga
     5341 ttgcctggtg gtcaccgatg tgactttcag aacgttaaca gacgaagaca tcgcgggcta
     5401 tgtcgccagc ggtgaaccgt tagataaagc aggtgcatac ggtattcagg ggctgggtgg
     5461 ctgttttgtc aggaagataa atggcagcta tcacgccgta gtcggcttac cgctggttga
     5521 aacgtatgaa ttattaagta attttaacgc actgcgtgag aaaagggata aacatgacgg
     5581 ctgaattgtt agtaaacgta acgccttcgg aaacgcgagt ggcgtatatt gatggcggta
     5641 ttctgcagga aattcatatt gaacgtgagg cgcgacgcgg aatagtaggc aatatctaca
     5701 agggtcgtgt aagtcgtgta cttccgggta tgcaggcggc ttttgtagat attgggctgg
     5761 ataaagccgc gtttcttcat gcatccgata tcatgccgca caccgaatgt gtggcgggtg
     5821 aagaacaaaa gcaattcacg gtgcgcgaca tctcggaact ggttcgtcag gggcaagatc
     5881 tgatggtgca ggtggtgaaa gatccgcttg gcactaaagg tgcgcgcctg accaccgata
     5941 tcacgctgcc ttctcgctat ctggtgttta tgccaggggc ttctcacgtt ggggtttccc
     6001 aacgtattga aagcgaatca gaacgtgaac gcctgaaaaa agtggtcgca gagtattgcg
     6061 acgagcaggg cgggtttatc atccgtaccg cagcggaagg ggttggcgag gctgaactgg
     6121 cctccgatgc cgcttatctg aaacgcgtct ggaccaaagt tatggagcgt aaaaaacgcc
     6181 cgcagacccg ttatcagctg tacggcgaac tggcgctggc gcagcgtgtt ctgcgtgatt
     6241 tcgccgacgc cgaactggac cgcattcgcg ttgactcacg cctgacttac gaagcattgc
     6301 tggagtttac ctcggagtac attcccgaga tgaccagcaa gctggagcat tacaccggac
     6361 gccagccgat tttcgatctc tttgatgtcg aaaacgaaat ccagcgagcg ctggaacgca
     6421 aagtagaact gaaatccggt ggttatctga ttatcgacca gaccgaagcg atgaccaccg
     6481 tggacatcaa taccggggcg tttgtcggtc atcgcaatct ggacgacacc attttcaata
     6541 ccaatattga agcgacgcag gctatcgctc gccagttacg gttgcgcaat ctgggcggga
     6601 ttatcattat tgatttcatc gatatgaata atgaagatca ccgccgacgc gtgctgcact
     6661 cgctggagca ggcattgagc aaagaccggg tgaaaactag cgttaatggt ttttcggcgc
     6721 tggggctggt ggagatgacg cgtaaacgca cccgcgaaag cattgagcac gtactgtgta
     6781 acgaatgccc aacctgccac ggtcgcggaa cggtgaaaac cgtggaaacg gtatgctatg
     6841 aaatcatgcg cgagattgtt cgtgtccacc atgcttacga ctccgaccgt ttcctggtct
     6901 atgcttctcc ggcagtagct gaagccttga aaggcgaaga gtcacactcg ctggcggaag
     6961 tggaaatttt cgttggtaaa caggttaaag tacaaattga accgctctat aaccaggagc
     7021 agtttgacgt cgtaatgatg taaacagatg ctgggccgcc atccggcaaa gggtttttga
     7081 gtcacatttt tagcagacaa ggagtgacgg gtgaggcgat tgccggggat tttactgctt
     7141 actggagccg cgctcgttgt gatcgctgcc ctgctggtta gcggcctgcg tattgcttta
     7201 ccgcatcttg acgcctggcg tccggaaatc ctcaacaaaa tagaatccgc gactggcatg
     7261 ccggtagaag ccagtcagct ctcagccagc tggcagaatt ttggcccgac gcttgaagca
     7321 cacgacatcc gtgcagaact aaaagatggc ggcgaatttt cggttaaacg cgttactctg
     7381 gcgctggatg tctggcagag cttgttacat atgcgctggc agtttcgcga cctcactttc
     7441 tggcagctgc gctttcgcac caacactcct atcaccagcg gtggtggtaa tgatagcctg
     7501 gaagccagtc acatcagcga tctgtttctt cgtcaatttg accatttcga tctccgcgac
     7561 agtgaagtca gttttctgac gccatccggt cagcgcgccg agctggcgat cccacaactc
     7621 acctggctga acgatccacg tcgacaccgt gcggaaggcc tggtaagcct ctccagcctt
     7681 accggacagc acggcgtgat gcaggtgcgc atggatttgc gcgatgatga ggggttgtta
     7741 agcaatggtc gcgtctggct acaggcggat gacatcgacc tgaagccgtg gctcggtaaa
     7801 tggatgcagg acaatattgc tctggaaacg gcacagttct cccttgaagg ctggatgacg
     7861 atcgacaaag gcgatgtaac cggcggtgac gtctggctga aacagggcgg tgccagctgg
     7921 ttgggcgaga agcaaacgca tacgctgtcg gtggataatc tgaccgcgca tattacgcgt
     7981 gaaaatccgg gctggcagtt ctctattccc gatacacgga tcacgatgga cggcaaaccc
     8041 tggccgagcg gagcattgac gctggcctgg ataccggaac aggacgttgg cggcaaagac
     8101 aataaacgca gtgacgaact ccggattcgc gccagtaatc tggagctggc aggcctggag
     8161 ggcatacgcc cgctggccgc gaaactttca cctgcactgg gtgatgtttg gcgctccaca
     8221 caaccgagcg gcaagattaa cactctggcg ctggatatcc cgcttcaggc ggcagacaag
     8281 acccgttttc aggcatcgtg gagcgatctg gcctggaagc aatggaaatt attaccgggt
     8341 gcggaacact tctccgggac gctttccggc agcgttgaaa atggtttgct taccgcgtcg
     8401 atgaagcagg caaagatgcc ttacgaaacg gtattccgtg cgccactgga aatcgccgac
     8461 ggccaggcaa ctataagctg gctgaacaat gacaaaggtt tccagctgga tgggcgtaat
     8521 attgacgtta aagccaaagc cgtccatgcg cgcggcggtt ttcgttacct gcaacctgct
     8581 aacgatgaac cctggctggg tattctggct ggcatcagta ccgatgatgg ttcacaagcc
     8641 tggcgctatt tcccggaaaa cttgatgggt aaagacctgg ttgattactt aagtggcgcg
     8701 attcagggcg gtgaagcgga taacgcgacg ctggtttatg gtggcaatcc gcaactcttc
     8761 ccctataaac acaacgaagg tcagtttgaa gtgctggtgc cgctgcgcaa cgcgaagttt
     8821 gccttccagc cggactggcc tgcattaact aaccttgata ttgaactgga ctttattaac
     8881 gacggtttat ggatgaaaac cgatggcgtt aatctgggcg gcgtgcgcgc gagtaatctc
     8941 accgcagtga tccctgacta ctcgaaagaa aaactgctga ttgacgctga cattaaaggt
     9001 ccgggtaaag ccgttggccc ttactttgat gagacaccgc tgaaagattc tctgggtgcg
     9061 accctgcaag aactccagct cgacggcgat gtgaatgctc gcttacatct tgatatcccg
     9121 ctgaacggcg aactggtaac cgcgaaaggt gaagtgacgc tgcgtaataa cagtctgttt
     9181 atcaaaccac tcgacagcac cctgaaaaat ttgagcggta aattcagctt tatcaatggc
     9241 gatctgcaaa gtgaaccact gacagcaagc tggtttaatc agccgttgaa cgtggatttt
     9301 tccaccaaag aaggggcaaa agcctaccag gttgcggtga atctcaacgg taactggcaa
     9361 ccggcgaaaa ccagcgttct gcctgaagcg gtgaacgaag cattgagtgg cagcgtggcg
     9421 tgggatggta aagtgggcat tgatctgcct tatcatgctg gcgcgaccta taacgtagag
     9481 atgaacggcg atctgaataa tgtgagtagt cacttacctt caccgttagc caaacctgcg
     9541 ggtgaaccac tagcggtaaa cgttaaggtt gatggcaatc tcaacagctt tgaattaacc
     9601 ggacaggctg gtgcggataa ccatttcaat agccgctggt tgcttggtca aaagctgacg
     9661 ctcgatcgtg ctatttgggc ggcagacagt aaaacgctcc cgccgttgcc ggaacaaagt
     9721 ggcgttgaac tcaatatgcc gccgatgaat ggtgccgagt ggctggccct gttccagaaa
     9781 ggcgctgcgg agagtgtcgg tggtgcagcg agtttcccac aacacataac gttacgtacg
     9841 cctatgttgt cactgggaaa tcagcaatgg aataacctga gtattgtttc gcaaccgacg
     9901 gcaaatggca ccctggttga ggcgcaaggg cgtgaaatca acgccacgct agcgatgcgt
     9961 aataacgcgc cgtggctggc gaatatcaaa tatctttatt acaacccgag cgtggcgaaa
    10021 actcgtggtg attcaacgcc gtcatcacct ttcccgacaa cggagcgcat taacttccgt
    10081 ggctggccgg acgcacaaat acgatgcaca gagtgctggt tctgggggca aaaattcggt
    10141 cgcattgaca gtgatatcac catttctggc gatacgttaa cgctgaccaa tggactgatt
    10201 gatactggtt tctcgcggct tactgccgat ggtgaatggg ttaataatcc ggagaatgaa
    10261 cgtacctcgc tgaaaggaaa actgcgcggg cagaaaattg atgccgccgc agaatttttt
    10321 ggtgtcacga cgcccatacg ccagtcgtca tttaatgtgg attacgattt acactggcgc
    10381 aaagcaccct ggcaaccaga tgaggcgacg ttgaatggca tcattcatac tcaactgggt
    10441 aaaggcgaaa ttaccgaaat caataccgga catgccgggc aattgctgcg cttattgagc
    10501 gttgatgccc tgatgcgtaa gctgcgtttt gatttcagag acacttttgg cgaagggttc
    10561 tattttgact ccattcgcag caccgcgtgg attaaagacg gggttatgca caccgacgac
    10621 acgctggtgg atggcctgga ggcggatatc gccatgaaag ggtcggtaaa tctggtacgt
    10681 cgcgacctga atatggaagc ggttgtcgca ccagagattt ctgcgacggt gggcgtggct
    10741 gcggcttttg cggttaaccc cattgttggc gcggcagtat ttgcagccag taaagtgctg
    10801 gggccgctgt ggagcaaagt ctccattttg cgctatcaca tttcgggtcc gctggacgat
    10861 ccgcaaatca acgaagtgtt gcgccaaccg cgtaaagaaa aagcgcaatg atttgacgag
    10921 ggcgcgtaat tgccccaatc tcatatgata atcgttgcca aaggccaacg agccggaaca
    10981 taactgtagg ccggataggg cgttcacgcc gcatccggca gccgtaaaaa atcctctact
    11041 gcagtaacta acgagtagca aaaacgatga gtctgaacct ggtaagtgaa caattgctag
    11101 cggcgaacgg cctgaaacat caggacttgt tcgcgatcct cggtcaactg gccgaacgtc
    11161 gccttgatta tggcgatctc tattttcagt cgagctatca cgaatcctgg gttttagaag
    11221 accgcattat taaagatggt tcttacaaca tcgatcaggg cgttggtgtg cgtgcaatca
    11281 gtggtgaaaa aaccggattt gcttacgctg accaaatcag cctgctggcg ctggaacaga
    11341 gtgcgcaagc ggcgcgcacc atcgtccgtg atagtggtga tggcaaagta cagacgctgg
    11401 gcgcggtaga gcatagcccg ttgtatacct cggtagatcc gctgcaaagc atgagccgtg
    11461 aagagaagct ggatatcctg cgtcgcgtcg ataaggttgc ccgcgaagcg gacaagcgcg
    11521 tacaagaagt gactgccagc ctcagcggcg tttatgaatt aattctggtt gcggccaccg
    11581 acggcacgct ggcggcggat gttcgtccgc tggtgcgtct ttccgtgagc gttctggtcg
    11641 aagaagatgg caaacgcgaa cgcggtgcca gtggcggcgg cggtcgtttt ggttatgagt
    11701 tcttccttgc cgatcttgac ggcgaagtcc gtgcggatgc atgggcaaaa gaagcagtgc
    11761 gtatggcgct ggtcaatctt tctgccgttg ctgcaccagc gggcaccatg ccggtagtac
    11821 ttggcgcagg ttggccgggc gtgctgttgc atgaagcggt aggtcacggt ctggaaggcg
    11881 acttcaaccg ccgcggcact tcagtattta gtgggcaggt cggggagctg gtggcttcag
    11941 aactgtgtac cgtggttgat gacggcacga tggtcgatcg ccgtggttcg gtggcgattg
    12001 atgacgaagg tacgccaggc cagtacaacg tgctgattga gaacggcatt ctgaaaggct
    12061 acatgcagga taaactcaac gcgcgtttga tggggatgac gccgactggc aacggtcgcc
    12121 gtgaatccta cgcccatctg cccatgccgc gtatgaccaa cacctatatg ctgccgggta
    12181 aatcgacccc gcaggaaatt attgaatccg ttgagtacgg tatctatgca ccgaactttg
    12241 gtggcggtca ggtggatatt acatccggca aattcgtttt ctccacttca gaagcgtatc
    12301 tgattgaaaa cggtaaagta acgaagccgg tgaaaggcgc gacgttgatt ggttccggta
    12361 tcgaaaccat gcagcagatt tcgatggttg gcaacgacct gaaactggat aacggcgtgg
    12421 gtgtctgcgg taaagaaggg caaagtttgc cggttggcgt gggccagcca acgctgaaag
    12481 ttgataacct gactgttggc ggtactgcgt aataatttca ttattttcag aaggataatt
    12541 aatctttcta cgtgcacgaa cggtcccctc gctcctctgg ggttagggtg aggggaaccc
    12601 gttggcacag gtttgaacaa cgtaacagta caatatgaat tacttctctt tcccgcgccc
    12661 atgcatctcc tgaaacaatt taccgacctc aacaaaataa tccgtcagcg agttgatcac
    12721 gacctgtacc ttcagcggca gtttatcttt ttcggtatac aacgcataaa ccgggcgtgg
    12781 atctgactgg taacgcggca gcaggatctc cagctcccca cgattgatct cgttgatcac
    12841 ccacatcagc ggcacgtagg cgatcccggc acccgccgtc agccagcgca ccagcgtcat
    12901 cggatcatta gtcacaaatc ttccttgtgg gatcaggcga gtcgagatcc cttccggtgc
    12961 gatcagttca aattcattgt cgggccgcac gctgtattca agccatgaat gactactcaa
    13021 atcggcgggt ttttccggta tgccgtattg tgtgagatag cttttcgcgg cgcacaccac
    13081 catcggcatc gcgcccagac ggcgggaaaa caggctggaa tcctgcaacg cgccgacgcg
    13141 gattaccaca tccagaccat cggcaatcag gtcgggggca ggaattccgg taaccagatt
    13201 gacgctcaaa cctgggtatt ctttcagcat ttttgctgtc agcccggcga gaacattttg
    13261 tgccatagtt gaagaacagc caatgcgcag cgtcccgatg ggggtgttat tgaaggcata
    13321 cagttgctca tgaacatcct gcacttcatg gagcatccga cggcagccct ggtagtaaat
    13381 tctgccggct tcagtcagac caatgctgcg tgtactacgg tttaacagct ttacctgcaa
    13441 ctcatcttcc agttttgaca ccgtctgact gatggacgaa acgctcatct gtagctgtct
    13501 ggcggcggcg gtaaaagagc caaattcaac tactttggca aacaccgaca tgcgttttag
    13561 tcgttccatt attcactctg acttaaaagt gatttagatc acataatata gataacagca
    13621 taacagttac gctaatatat taaatatcaa tctacagcaa tgttgctctc gcccggcttt
    13681 ccatgccctt ctctgcggcg acagatgctg aaaataataa cgcctgctct ctctatacaa
    13741 ccaaggtcga catgagtctg tttcccgtta tcgtggtgtt tgggctgtcc ttcccaccga
    13801 tattttttga attgctttta tcactggcga ttttctggct ggtgcgccgg gtacttgtgc
    13861 caacaggtat ctacgacttt gtctggcatc cggcgttgtt caacaccgcg ctctattgct
    13921 gcttgtttta tttgatatcg cgactgttcg tttgaggttg aagtgaaaac actaataaga
    13981 aaattctccc gtacggccat cacggtcgta ttagtcattc tggccttcat cgcaattttt
    14041 aatgcctggg tctattacac cgaatccccg tggacgcgtg acgcgcgctt tagcgctgat
    14101 gtcgttgcga tcgcgccgga cgtttctgga ctcattaccc aggtgaatgt tcatgataac
    14161 cagctggtga aaaaaggaca ggtactgttc accatcgacc agccgcgcta tcaaaaggcg
    14221 cttgaggaag cgcaagccga tgttgcttat tatcaggtac tggcacagga gaaacgccag
    14281 gaggccggac gtcgtaatcg tctcggtgtg caggcgatgt ctcgcgaaga gatcgaccag
    14341 gccaacaacg tactacaaac ggttctgcat cagttagcga aagcgcaggc gacccgcgat
    14401 ctggcaaaac tggatcttga acgcacggtg atccgcgcgc cagcggatgg ctgggtgacc
    14461 aacctcaacg tctataccgg tgagtttatt actcgtggat caacggcggt tgcgctggtg
    14521 aaacagaact ctttctatgt actggcctat atggaagaaa ctaagctgga aggggtgcgc
    14581 cctgggtatc gtgcagagat cacgccgctt ggcagtaaca aagtgctgaa agggactgtt
    14641 gatagtgttg ccgctggggt caccaacgcc agcagcacgc gtgacgacaa agggatggcg
    14701 accatcgact ctaaccttga atgggtgcgt ttggcgcaac gtgttccggt tcgtattcgt
    14761 ctcgacaacc agcaagagaa catctggcct gcgggcacca ctgctacagt ggtggtcact
    14821 ggcaaacaag atcgcgacga aagccaggat tcgttcttcc gtaaaatggc ccatcgcctg
    14881 cgtgagtttg gttaatcacg atgggtattt tctccattgc taaccaacat attcgctttg
    14941 cggtaaaact ggcgaccgcc attgtactgg cgctgtttgt tggctttcac ttccagctgg
    15001 aaacgccacg ctgggcggta ctgacagcgg cgattgttgc tgccggtccg gcctttgctg
    15061 cgggaggtga accgtattct ggcgctattc gctatcgtgg ctttttgcgc atcatcggca
    15121 catttattgg ctgtattgcc ggactggtga tcatcattgc gatgatccgc gcaccattat
    15181 tgatgattct ggtgtgctgt atctgggccg gtttttgtac ctggatatcc tcgctggtac
    15241 gaatagaaaa ctcgtatgcg tgggggctgg ccggttatac cgcgctgatc attgtgatca
    15301 ccattcagcc tgaaccattg cttacgccgc agtttgccgt cgaacgttgt agcgagatcg
    15361 ttatcggtat tgtgtgtgca attatggcgg atttgctctt ttctccgcga tcgatcaaac
    15421 aagaagtgga tcgagagctg gaaagtttgc tggtcgcgca atatcaatta atgcaactct
    15481 gtatcaagca tggcgatggt gaagttgtcg ataaagcctg gggcgacctg gttcgacgca
    15541 ccacggcgct acaaggtatg cgcagcaacc tgaatatgga atcttcccgc tgggcgcggg
    15601 ccaatcgacg tttaaaagcg atcaatacgc tatcgctgac gctgattacc caatcctgcg
    15661 aaacttatct tattcagaat acacgcccgg aattgatcac tgatactttc cgcgaatttt
    15721 ttgacacacc ggtagaaacc gcgcaggacg tccacaagca gctcaaacgc ctgcgaagag
    15781 ttatcgcctg gaccggggaa cgggaaacgc ctgtcaccat ttatagctgg gtcgcggcgg
    15841 caacgcgtta tcagcttctc aagcgcggcg ttatcagtaa cacaaaaatc aacgccaccg
    15901 aagaagagat cctgcaaggc gaaccggaag taaaagtaga gtcagccgaa cgtcatcatg
    15961 caatggttaa cttctggcga accacacttt cctgcattct gggaacgctt ttctggctgt
    16021 ggacgggctg gacttccggt agtggtgcaa tggtgatgat tgcggtcgtg acgtcactgg
    16081 caatgcgttt gccgaatcca cgcatggtgg cgatcgactt tatctacggg acgctggccg
    16141 cgctgccgtt agggctgctc tactttttgg tgattatccc taatacccaa cagagcatgt
    16201 tgctgctgtg tattagcctg gcagtgctgg gattcttcct cggtatagaa gtacagaaac
    16261 ggcgactggg ctcgatgggg gcactggcca gcaccataaa tattatcgtg ctggataacc
    16321 cgatgacttt ccatttcagt cagtttctcg acagcgcatt agggcaaatc gtcggctgtg
    16381 tgctcgcgtt caccgttatt ttgctggtgc gggataaatc gcgcgacagg actggacgtg
    16441 tactgcttaa tcagtttgtt tctgccgctg tttccgcgat gactaccaat gtggcacgtc
    16501 gtaaagagaa ccacctcccg gcactttatc agcagctgtt tttgctgatg aataagttcc
    16561 caggggattt gccgaaattt cgcctggcgc tgacgatgat tatcgcccac cagcgcctgc
    16621 gtgatgcgcc gatcccggtt aacgaggatt tatcggcgtt tcaccgacaa atgcgccgca
    16681 cagcagacca tgtgatatct gcccgtagcg atgataaacg tcgtcggtac tttggtcagt
    16741 tgctggaaga actggaaatc taccaggaaa agctacgcat ctggcaagct ccaccgcagg
    16801 tgacggaacc ggttcatcgg ctggcgggga tgctccataa gtatcaacat gcgttgaccg
    16861 atagttaagt caaaaccgac gccaaaagcg tcggtttttt catggctata cttagcgatg
    16921 cacggcagaa ctcgccgcga aacgtgacgg tggcaacaga tgaatattta tacctttgat
    16981 tttgatgaga ttgagagtca ggaggatttt tatcgtgact ttagccaaac ctttggtctg
    17041 gcgaaagata aggtacgcga tctcgactca ctatgggatg tgttaatgaa cgatgtcctg
    17101 ccgctaccac ttgagattga atttgttcat ctgggagaga aaacgcgtcg ccgttttggc
    17161 gcgttaatat tgctgtttga tgaggcagag gaagagctgg aagggcattt gcgttttaat
    17221 gttcgtcatt agcgcaaaaa aaagcccccg aaccgggggc aatatcgtcg gacaagacga
    17281 tgagggttta tttgtacagt tcagccgtag cgtgccaggt gtcaccgcta cgagcttcag
    17341 taatctggta ggccgttgcg cctttctctt ccgctttttt gttcagcatt tcacgcatat
    17401 ccattggcga agacgccaca ccacttacgg atacggtccc gattgcttca cgattttgtg
    17461 cttgtgcagc atcaatggag tcggcagcga atgcaccgaa agagagaaca gaaagtacgc
    17521 ttaatgcagc aacagtggtt ttgattttca tgatttttac ctcgacataa tcttttagct
    17581 gggcctttgt ttcgtgaccc atatcacaaa atcaagtata cactaattat tgaactaatt
    17641 aataccacgc taactgtttc agttgatact aagtgtta
//
LOCUS       DAPHDW010000091        17629 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.092, whole genome shotgun sequence.
ACCESSION   DAPHDW010000091 DAPHDW010000000
VERSION     DAPHDW010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17629
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.092"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(266..1279)
                     /locus_tag="RH057_003027"
     CDS             complement(266..1279)
                     /locus_tag="RH057_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HDV3129363.1"
                     /translation="MSSWLAMLQDAAEMFVFLAVELSLLFIVISAGVSLIRQKVPDHK
                     IQQMMGARKGRGYLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLN
                     PIIVGLMWVTFGWKVTLLYAIIATGVSVLASIILDSLGFERHIIASKSSSANCCAPAK
                     ASAKTLPGTTYTPIEVSCCSPTAKAIEKPVVNCCNTKAVASINPIKLATKDALQQFKD
                     VLPYLLLGVLIGSFIYGFIPSAWIAAHAGADNPFAIPLSAVVGIPLYIRAEAVIPLAS
                     VLMTKGMGLGALMALIIGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQ
                     LLF"
     gene            1386..1682
                     /locus_tag="RH057_003028"
     CDS             1386..1682
                     /locus_tag="RH057_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3129364.1"
                     /translation="MQLEEVAKALKELGHPTRLFIFKHLVKAGEQGLPVGELQKQLGI
                     PGSTLSHHISALVSVGLVKQNRESRTLMCVSQYAMLEAIIEFLREECCVNSKIA"
     gene            complement(1816..2241)
                     /gene="arsC"
                     /locus_tag="RH057_003029"
     CDS             complement(1816..2241)
                     /gene="arsC"
                     /locus_tag="RH057_003029"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HDV3129365.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIYYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEILPDAQKGAFSKEDGEKVVDEAGKRLK"
     gene            complement(2254..3543)
                     /gene="arsB"
                     /locus_tag="RH057_003030"
     CDS             complement(2254..3543)
                     /gene="arsB"
                     /locus_tag="RH057_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HDV3129366.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKAGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(3597..3950)
                     /gene="arsR"
                     /locus_tag="RH057_003031"
     CDS             complement(3597..3950)
                     /gene="arsR"
                     /locus_tag="RH057_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HDV3129367.1"
                     /translation="MSFLLPIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQ
                     PKISRHLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKVQAIVRN
                     LARQNCSGDSKNICS"
     gene            4690..4773
                     /locus_tag="RH057_003032"
     CDS             4690..4773
                     /locus_tag="RH057_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HDV3129368.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(4827..6179)
                     /gene="gorA"
                     /locus_tag="RH057_003033"
     CDS             complement(4827..6179)
                     /gene="gorA"
                     /locus_tag="RH057_003033"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HDV3129369.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGSEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(6251..7093)
                     /gene="rlmJ"
                     /locus_tag="RH057_003034"
     CDS             complement(6251..7093)
                     /gene="rlmJ"
                     /locus_tag="RH057_003034"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HDV3129370.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSEFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLPWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            7296..9338
                     /gene="prlC"
                     /locus_tag="RH057_003035"
     CDS             7296..9338
                     /gene="prlC"
                     /locus_tag="RH057_003035"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HDV3129371.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPIEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAELAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELAQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDDCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            9344..10096
                     /gene="rsmJ"
                     /locus_tag="RH057_003036"
     CDS             9344..10096
                     /gene="rsmJ"
                     /locus_tag="RH057_003036"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HDV3129372.1"
                     /translation="MQICLIDETGTGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(10145..11614)
                     /gene="dtpB"
                     /locus_tag="RH057_003037"
     CDS             complement(10145..11614)
                     /gene="dtpB"
                     /locus_tag="RH057_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
                     /protein_id="HDV3129373.1"
                     /translation="MNTTTPMGMLQQPRPFFMIFFVELWERFGYYGVQGVLAVFFVKQ
                     LGFSQEQAFVTFGAFAALVYGLISIGGYVGDHLLGTKRTIVLGALVLAIGYFMTGMSL
                     LKPDLIFIALGTIAVGNGLFKANPASLLSKCYPPKDPRLDGAFTLFYMSINIGSLIAL
                     SLTPVIADRFGYSVTYNLCGAGLIIALLVYIACRGMVKDIGSEPDFRPMSFSKLLYVL
                     LGSVVMIFVCAWLMHNVEVANLVLIVLSIVVTIIFFRQAFKLDKTGRNKMFVAFVLML
                     EAVVFYILYAQMPTSLNFFAINNVHHEILGFSINPVSFQALNPFWVVLASPILAGIYT
                     HLGNKGKDLSMPMKFTLGMFMCSLGFLTAAAAGMWFADAQGLTSPWFIVLVYLFQSLG
                     ELFISALGLAMIAALVPQHLMGFILGMWFLTQAAAFLLGGYVATFTAVPDNITDPLET
                     LPVYTNVFGKIGLVTLGVAVVMLLMVPWLKRMIATPESH"
     gene            complement(11932..12366)
                     /gene="uspA"
                     /locus_tag="RH057_003038"
     CDS             complement(11932..12366)
                     /gene="uspA"
                     /locus_tag="RH057_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HDV3129374.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            12757..13092
                     /gene="uspB"
                     /locus_tag="RH057_003039"
     CDS             12757..13092
                     /gene="uspB"
                     /locus_tag="RH057_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HDV3129375.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(13163..14662)
                     /gene="pitA"
                     /locus_tag="RH057_003040"
     CDS             complement(13163..14662)
                     /gene="pitA"
                     /locus_tag="RH057_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitA"
                     /protein_id="HDV3129376.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSMLL
                     AAIIWNLGTWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLSIFGSLI
                     VSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRIALILSA
                     IGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNVEAYFEQ
                     HPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVESYDKLSL
                     DQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPVSTTHVL
                     SSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWLSLQFL"
     gene            14894..16096
                     /locus_tag="RH057_003041"
     CDS             14894..16096
                     /locus_tag="RH057_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HDV3129377.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQAGRRVLLIDNGKKPGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLELNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGIVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVRDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSAWACAQ
                     DLIAAKSS"
     gene            complement(16412..17464)
                     /locus_tag="RH057_003042"
     CDS             complement(16412..17464)
                     /locus_tag="RH057_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 domain-containing protein"
                     /protein_id="HDV3129378.1"
                     /translation="MNIYIGWLFKLIPLIMGLICIALGGFVLESSGQSEYFVAGHVLI
                     SLAAICLALFTTAFIIISQLTRGVNTFYNTLFPIIGYAGSIITMIWGWALLAGNDVMA
                     DEFVAGHVIFGVGMIAACVSTVAASSGHFLLIPKNAAGSKSDGTPVQAYSSLIGNCLI
                     AVPVLLTLLGFIWSITLLRSADITPHYVAGHVLLGLTAICACLIGLVATIVHQTRNTF
                     STKEHWLWCYWVIFLGSITVLQGIYVLVSSDASARLAPGIILICLGMICYSIFSKVWL
                     LALVWRRTCSLANRIPMIPVFTCLFCLFLASFLAEMAQTDMGYFIPSRVLVGLGAVCF
                     TLFSIVSILEAGSAKK"
BASE COUNT         4580 a         4444 c         4536 g         4069 t
ORIGIN      
        1 aaaaatgtgt tgtattagcg actgaaatct gccttttaaa aaggaggagg gtactgagac
       61 aaagagtcat tgcttctccg ataaaatgtc agagaagcat gattacgtgg ttattgtctg
      121 ttgatgactt gttgaccgag ccgtcatcat tctcaatgca ttagttaaat catttgtgat
      181 ttttaaggat tatcctgtct atgattttct acttagaagg atatggcact aactatgcat
      241 cttatagttt actgctggca tctatttaaa atagcaattg agtcaaatag cccatcaaga
      301 tagccatacc taatataacc gtcaggaatg cagctatcat cggcattctg aacattgatt
      361 taagtaatat cacttccgtc aggcttgcgc cggcactgcc gatgattaat gccattaatg
      421 ctcccagacc cattcctttt gtcatcagaa cagatgccag agggataacc gcctcagccc
      481 ggatatacag cggaataccg acaacggcgc ttaatgggat ggcgaaggga ttatctgctc
      541 ctgcatgagc ggcaatccac gctgagggaa taaagccata aataaaagag cctattaata
      601 cccctaacaa aagatatggc agtacatctt taaactgttg caacgcatct ttggtggcta
      661 gttttatggg attaatactt gccactgctt tggtattgca acagttaacg acgggtttct
      721 caatggcttt agccgttgga ctacagcaac tcacttctat tggcgtatat gtcgtccccg
      781 gcaaagtttt tgccgacgct ttggctggag cacaacaatt tgctgatgag cttttactgg
      841 caatgatatg acgttcaaac cccagagaat ccaggataat actggcaaga acggagacgc
      901 cggtggcgat aatcgcgtac aacaaggtga ctttccaacc aaaggtcacc cacattagtc
      961 cgacgataat gggattaagt aatggtgaaa caaataaaaa agtgagagtc ggaccaaacc
     1021 cggctttcgc tgacaacaat ccacgtagca tgggtattgt cgagcaacta cagaacgggg
     1081 taacggctcc caacagagca gccaggagat agcctctccc ttttctggcc cccatcatct
     1141 gctggatttt atggtctggc accttttgtc ttatcaggct gacaccggca ctaatcacta
     1201 taaacagcaa cgaaagctcg acggcgagaa acacaaacat ctctgcggca tcttgcagca
     1261 tcgcaagcca actactcata cgtaacctca ttggataaat ctatatttcc agaataatcg
     1321 aaatgaaatt cagatcaaga tatttctggt attatcgaaa tatcaaccag acagaggggt
     1381 gtcatatgca gttggaagaa gtcgctaaag cgctgaaaga gctaggtcac ccgacccgtt
     1441 tgttcatatt taaacatctt gtcaaagccg gggaacaagg gttgccggtc ggtgagttgc
     1501 aaaagcagct tgggatacct ggctccacac tcagccacca tatatcagca ctggtatcgg
     1561 taggactggt caaacaaaac cgtgagagtc ggacgctgat gtgtgtttcg caatacgcaa
     1621 tgctggaagc gataattgaa ttcttacgcg aggagtgctg cgtaaatagc aaaattgcct
     1681 gatgcgctac gctctacata tttcatgcca tatattgaat ttgcacggtt ttgtaggtcg
     1741 gataagacgc gttagcgtcg catccgacat aaacaaagcg cactttacca actatgggca
     1801 gtggatatcg ccgctttatt tcaaccgctt acccgcttca tcaaccactt tctcgccatc
     1861 ttccttggag aatgcgcctt tttgcgcatc tggcagaatt tccagcacca cttctgaagg
     1921 gcggcacagg cgagttccca gcggcgtcac cacaatcggg cgattaatca gaatcgggtg
     1981 ctgaagcata aagtcgatta accgatcgtc agtaaattta tcttctgcaa ggcccagttc
     2041 ctcatacggc tcgacgtttt tacgcaacag cgcgcgtacg gaaatcccca tatcggcaat
     2101 gagtttgacc agctcatcgc gcgttggcgg tgtttccaga taatagataa tggtcggttc
     2161 tgtgccgctg ttgcggatca tctccagcgt attacgcgac gtgccgcagg ccgggttgtg
     2221 ataaatggta atgttgctca tatcagtatc tcattacaaa gtgaaagaga gacgtagcgc
     2281 cagcgcagcc agcgtcacaa acagcacagg cagggtcatg ataatccctg tacggaagta
     2341 atatccccag ctgatagtca tattcttctg cgaaagtacg tgcagccaga gtagcgtagc
     2401 caggctacca attggggtaa ttttcggtcc caaatcgcag ccaatcacat tggcataaac
     2461 catcgcttct ttgataacgc cagatgccgt gctgccatca atggacaacg cgccaaccag
     2521 taccgtcggc atattgttca taatagaaga gaggaaggcg gtgaggaatc cggtgccgag
     2581 cgtcgcggcc cacaggccgt tatccgccag cacgttgagt acgccagaaa gatattccgt
     2641 taatccggca ttgcgcaggc cataaaccac cagatacatg ccgagcgaga agatgacaat
     2701 ctgccagggg gcaccgcgca ggactttacc cgtattaatc gcatgaccgc gtttagcgac
     2761 gacaaataat atcagcgcgc ccacagctgc aatggcgctc accggaatgc cgagcggttc
     2821 caggacgaaa aatcccacca gcagaagcag taaaacaacc cagccagctt tgaacgtagc
     2881 aggatctttg atcgcttctg cgggagattt cagcagcgcc atatcgtagt tctgcggaat
     2941 atctttgcga aaatagagat gtaacatcac cagcgtggca acaatcgcgg cgatatccac
     3001 cggcaccatc accgaggcgt attcgcgaaa gccgaggcca aagaaatcag cggaaacgat
     3061 attcaccagg ttggagacaa taagcggcag gctggcggta tcggcaatga atccggccgc
     3121 catcacgaac gccagcgtag tgcctttact gaaccctaaa gccagcagca tggcgatgac
     3181 aatcggtgtc aaaataagcg ccgcgccatc attggcaaac agggcggcaa cggcagcacc
     3241 gagcaggaca atccaggtaa acagcaagcg accacgacca ttaccccagc gtgagacgtg
     3301 cagcgccgcc cattcaaaaa agccggactc atccagcagc aggctgatga taatgacggc
     3361 gataaacgca gccgtcgcgt tccagacgat attccacacc accggaatat cacccggatg
     3421 gaccacgccc gtaactaacg ccagtactgc gccgagcgtt gcactccagc cgatgcctaa
     3481 acctttcggc tgccagataa ccaatacgat ggtcaggaca aagatagcgc ctgccagtaa
     3541 cataatgcct cccggataaa acacatctga aaattcatat gtgtttagct aaatttttaa
     3601 ctgcaaatgt tcttactgtc cccggaacag ttttgtcgag ccaggttgcg gacaatcgcc
     3661 tgaacctttt cctgttcaca tcgccaggcc tcatcaataa ttttcgccgc ccatgctgga
     3721 atatgcggtg ataagcggta atgaacccac ttaccttgct tgcggtccag caatagcccg
     3781 ctttcacgca gcaatgccag gtggcgggag atcttgggct gcgactggtc gagagcagtg
     3841 cagagatcgc agacgcataa ctctcccagt tcgctgagca gtaaaacgat gcccagacgg
     3901 gtttcatcag caagaatttt gaacaattgg atgggtaaca gaaatgacat attgcgctcc
     3961 tgattgttgc aggtagtgtc tctcttcgaa gcggataagt caaaaacata tatgacttaa
     4021 cgaatgtgta agtgcagagg aaggtaatag gtgtgaattt tgagttggct attcatttga
     4081 aaagaggtct gaatcaggac gtaaaaaaac cgactttgcg tcggtttttt actttccagc
     4141 cctgagttgg tggctctggc tggagtgaga gagttcttat ctaacagctc aatactatta
     4201 gccagtggct aacattgcaa gaattagcga tttcttcagc tggcggaggg ggcacttcgg
     4261 tatatatcgc aatgacgatt tatatacttt cactgggtca tcgtcatatt aagccttgtc
     4321 cgaatccgga tgtaaaaaaa ccgactttgc gtcggttttt tactttccag ccctgagttg
     4381 gtggcactgg ctggagtgag aatgccatta tctaacgcat caatgctaat accatactga
     4441 aactattgca aggacatgct ggttttataa cctgaatgta ctgtatgatt atccagttag
     4501 ctctgaggca ttttcactct ggtaatgcgc ataaacgctt tcaaagtcct ggtcagaagt
     4561 acgggtggtg ccgttaactg atgctctggc cggagtgaga atgccattat ctaacaatag
     4621 ggcatgcgcc gtgacaggca gtggatgagt aagcggatgc attctcactc catcgcatgg
     4681 agaaaacggg tgattgataa agcaatcatc gttcttgggg cgttaattgc gctgctggaa
     4741 ctgatccgct ttctgcttca gcttctgaac tgatagcgga aacgtaatta agggctaaga
     4801 gcacactact cttagccctt taacatttaa cgcattgtca cgaactcttc tgccgccgtt
     4861 gggtgaatgg cgacggtatt gtcgaagtct tttttggttg cacccatctt cagcgccacc
     4921 gcgaagccct gcaacatttc gtccatacca aagccaatgc cgtgaatacc gacaatcttc
     4981 tcttccgatc caacgcacac cagcttcatg cggcacggct ggcggtgagt ggtgacggcg
     5041 gtatacatcg cggtgaaaga ggatttatac actttcacct gatcgtcgcc atactgctcg
     5101 cgcgcctgcg gttccgttaa accaacagta ccaatcggcg gatggctgaa gaccacggtc
     5161 ggaatgttgc tgtaatccag atgctcatcc ggcttgttat taaacaggcg ttcagagaga
     5221 cggcgacccg ctgcaactgc caccggtgtc agctccactg cacccgtgtt atcgcccacc
     5281 gcgtaaatac cttcaatatt ggtgttttga tatttatcga cgacgatata gcctttttcg
     5341 ttagttttaa cgccagcggc ttccaggttg atgttgtcat tggcaggctc gcgaccaatc
     5401 gcccaaatca ggcaatccac cgtttcactg cgaccatctt ccagctccag cgtcaggcta
     5461 ccatcggcat ttttcactac cgctttcggg atggcgttgg tgtgcagctg cgggccttcg
     5521 gcgttcatca cttcgaccag cgtttcggaa atcatcgggt cgaagctgcg cagcggcgca
     5581 tgtttacgca caaacagatg cgttttcgcg ccgaggccgt taatcacgcc cgccagctca
     5641 acggcgatgt aacccgcgcc aacaaccgcc acgcgctctg gcaaagcagg aagggcgaag
     5701 aagccatcag aatcaatacc gtattccacg cccggaatat ccgggtggct cggacgaccg
     5761 cctgtggcga tcagaatatg atcggccgtg atggtttcgc cgtttacctc cagcgttttg
     5821 gcatcaacga agcgggcaaa gcctttgatt acatcaacgt tatttttacc gagcacgttt
     5881 tcataggaag tatgaatacg gtcgatatag gcggtacggc tggcgatcaa cgtttcccag
     5941 ttgaatttat tgatagtggt atcaaaacca taatccgggc cgtacatatg gatcgcttca
     6001 cggatttgcg ccgcgtgcca catcactttt ttcggcacac agccaacatt tacgcaggtg
     6061 ccgcccagct ctttggcttc aatcagcgca catttctggc cgtacatagc cgcgcggttg
     6121 atggaggcga taccgccgct gccgccgccg atggcgatgt aatcatagtg tttagtcatg
     6181 acaaagtgtc cttatcgttg attaccgcga ttgtagcgcg agacgtaata ggtgccagca
     6241 atggctgcaa ttactccggc acgatccagc ttacggtggc gtgcccggtg cctgccggaa
     6301 ccagtttgct gtgcagccac ggcagcacgt tattcatctg ctgttccagt ttccacggcg
     6361 ggttaatcac aatcatgccg gaagcggtca tgccacggcg atcgctgtct ggcagtaccg
     6421 ccagttcaat ttgcagaatt ttgcgaatac cggtcgcttc cagatcgtgg atcatgcgct
     6481 taatttgctg acgcagcacc accggatacc acagtgcgta agtaccagtg gcgaaacgtt
     6541 tgtaaccttc tgctatcccg ctgaccaccg cctgatagtc ggttttcatt tcatacggcg
     6601 ggtcgataag gattaaacca cggcgggaaa ccggcggcag tttggcctta agctgctgga
     6661 aaccatcggc tttttcgacg cgcgcacggc tgtctttctg aaattcagaa cgcagcaacg
     6721 ggtaatcgct cgggtgcagt tcggtcagtt gcaggctgtc ttgttcgcgc agtagctggc
     6781 gagcaatcaa tggcgaaccg gggtagtaac gcaactgacc gctacggttg aagtgtttta
     6841 ccacattgat gtacgcctcc agttctgcgg gcaaatcgtc ctgctgccag atacgggcga
     6901 tgccttcgag atattcgccg gtacgctcgg catgttcgct gcctaactga taacgcccgg
     6961 cccctgcgtg ggtgtcgaga tagagaaacg gtttatcttt ctctttcagc gactcgatga
     7021 tcaggctctg aacggtatgt ttaaggacgt cggcgtggtt gccagcgtga aagctgtggc
     7081 gataactgag catgggtaaa ggtgttccgg taagtaaaaa tcggcccgta ttcggggcgc
     7141 acaaaagcgt atcaggacag tataccgaaa agaggccgcc gccgcgaaag cgtaacgttt
     7201 ctcattgaaa ttcactacac ttaaccccat gctacacaca ttatgtaaag cgcctgttga
     7261 gcgcttcctt aacctcttta accaggactg cgcgaatgac gaatccgtta ctgactccct
     7321 ttgaattgcc tccgttttct aaaattctcc cggaacatgt cgttccagcc gtgactaagg
     7381 cgctgaacga ctgccgcgaa aatgtggagc gcgtagtagc gcaaggagca ccgtacacct
     7441 gggaaaatct ctgccagccg ttggcggaag tggacgatgt gctggggcgt atcttctccc
     7501 cggtcagcca cctgaactcg gtgaaaaata gcccggaact gcgtgaagcg tacgaacaaa
     7561 ccctgccgct gctgtcggaa tacagcacct gggtagggca acatgaaggg ctgtataaag
     7621 cgtatcgcga cctgcgcgat ggcgatcatt acgccacgct gaacacggct cagaaaaaag
     7681 cggttgataa cgcactgcgc gacttcgaac tctctggcat cggtctgcca atagagaaac
     7741 aacaacgcta cggtgagatt gccactcgtc tttccgagct gggcaaccag tacagcaaca
     7801 acgtcctcga tgcgacgatg ggctggacca aactcgttac cgacgaagcg gagctggcgg
     7861 ggatgcctga aagcgcgctg gctgcggcaa aagcccaggc cgaagcgaaa gagctggaag
     7921 gctacctgct gacgctggat atcccaagct acctgccggt aatgacctac tgcgacaacc
     7981 aggccttgcg tgaagagatg tatcgcgctt acagcacccg cgcctccgat caaggcccga
     8041 acgccggtaa atgggataac agcaaggtga tggaagagat cctcgctctg cgtcacgaac
     8101 tggcgcaact gctgggcttt gaaaactacg cctttaaatc ccttgccact aaaatggcag
     8161 aaaacccgca gcaggtgctg gatttcttaa ccgatctggc aaaacgcgcg cgtccgcaag
     8221 gcgaaaaaga gctggcacaa ctgcgcgcct ttgccaaagc cgaatttggc gtcgatgagt
     8281 tgcagccgtg ggatatcgcg tactacagcg aaaaacaaaa acagcacctc tacagcatca
     8341 gtgacgaaca gctgcgtccg tacttcccgg aaaacaaagc ggttaacggt ttgtttgaag
     8401 tggtgaaacg tatttacggc atcaccgcta aagagcgtaa agatgttgat gtctggcacc
     8461 cggatgtacg tttcttcgaa ctgtatgacg agaacaacga actgcgcggt agcttctacc
     8521 tcgacctgta tgcccgtgaa aacaagcgcg gcggggcgtg gatggatgac tgcgtaggcc
     8581 agatgcgcaa agctgacggt tctctgcaaa aaccggtcgc gtatctgacc tgtaacttta
     8641 accgcccggt aaatggtaaa ccggcgctgt ttacccatga cgaagtgatc accctgttcc
     8701 acgagttcgg tcatggtctg catcatatgc tgacccgcat cgaaaccgcc ggtgtatccg
     8761 ggatcagtgg ggtgccgtgg gatgcggtcg aactgccgag tcagtttatg gaaaactggt
     8821 gctgggagcc tgaggcgctg gcgtttatct ctggtcacta cgaaaccggc gaaccgctgc
     8881 cgaaagagtt gctggataaa atgctggcgg cgaagaacta ccaggcggcg ctgtttattc
     8941 tgcgccagct ggagttcggc ctgttcgatt tccgcctcca tgccgagttc cgcccggatc
     9001 agggagcgaa aatcctcgaa actctggcag aaatcaagaa actggttgcc gtagtaccgt
     9061 ctccatcctg gggccgtttc ccgcacgctt tcagccatat tttcgccggt ggttatgccg
     9121 caggttacta cagctacctg tgggctgacg tactggcggc agatgctttc tcgcgctttg
     9181 aggaagaggg cattttcaac cgtgaaaccg ggcagtcgtt cctcgacaac attctgagcc
     9241 gtggcggttc agaggagccg atggatctgt tcaaacgctt ccgtggtcgt gagccgcagc
     9301 tggatgcgat gctggagcat tacgggatca aaggttagtt tttgtgcaga tttgcttaat
     9361 tgatgaaaca ggcaccggag acggtgcctt atctgttctg gcggcccgct gggggctgga
     9421 gcacgatgaa gacaacctga tggcgctggt gttaacgccg gaacatctgg aattgcgcaa
     9481 gcgtgatgag ccaaaacttg gcggcatctt tgttgatttt gttggcggag cgatggcgca
     9541 ccgacgcaaa ttcggcggtg gtcgcggtga ggcggtggcg aaagcggtgg gcattaaagg
     9601 cgattatttg ccggatgtgg tggacgccac cgcagggctg gggcgcgatg cctttgtgct
     9661 ggcttcagtc ggttgccgcg tgcggatgct ggagcgtaat ccagtcgttg ccgccttgct
     9721 cgacgacggc ctggcgcgtg gttatgcgga tgcggaaatc ggcggctggt tgcaggagcg
     9781 gttgcagtta attcacgcct ccagcctgac ggcgctgact gatattaccc cgcgcccgca
     9841 ggtggtttat ctcgacccga tgttcccgca taagcagaaa agcgcgctgg tgaaaaaaga
     9901 gatgcgtgtc tttcagtcgc tggtggggcc ggatcttgat gccgatggat tgctggagcc
     9961 tgcgcggctg ctggcgacca aacgcgtggt ggtaaagcgc ccggactacg cgccaccgct
    10021 ggcgaatgtc gccacgccaa acgcggtagt cactaaaggg cataggtttg atatttatgc
    10081 cggaacgccg gtgtaaaagt aaagcagggt agcgataacg ctaccctgct ttctgcaaga
    10141 ataattaatg gctttccggc gtcgcaatca tgcgtttcag ccacggcacc atcaacagca
    10201 tcactactgc aacgcccagc gtgaccagac caatcttacc aaacacgttg gtatagacgg
    10261 gcaacgtctc aagcggatcg gtaatgttgt ccggcaccgc ggtaaatgtt gccacatagc
    10321 cgcccagcaa gaacgcggca gcctgcgtca ggaaccacat cccgagaata aagcccatca
    10381 aatgctgcgg caccagggca gcaatcatcg ccagacccag ggcgctaata aacaattcac
    10441 ccaggctctg gaacaagtaa accagcacga taaaccacgg cgatgtcagt ccttgtgcat
    10501 cagcaaacca cattcccgca gctgccgccg tcaaaaagcc cagtgagcac ataaacatgc
    10561 cgagagtaaa tttcatcggc atcgagaggt ctttgccttt gttacccaga tgcgtgtaaa
    10621 tgcctgccag tattgggctg gcgagtacca cccagaacgg gttaagcgcc tggaagctga
    10681 ccgggttgat ggaaaagccg agaatttcat gatgcacgtt gttgatggca aagaagttca
    10741 gcgacgttgg catctgggcg tagagaatgt aaaacaccac cgcttcgagc atcaggacaa
    10801 aggcgacaaa cattttattg cgcccggttt tatccagctt gaatgcctga cgaaagaaga
    10861 tgatggtgac gacgatggag agaacaatca gcaccagatt ggcgacttct acgttgtgca
    10921 tcagccatgc acagacgaag atcatcacca cgctgccaag taacacgtac aacagtttgc
    10981 tgaagctcat tggccggaag tcgggttcag aaccaatgtc tttcaccatt ccacgacagg
    11041 cgatgtaaac cagtaatgcg ataattaacc ccgccccgca caggttgtag gtgactgaat
    11101 aaccgaatct atcagcgatc acaggggtca gcgataacgc tatcaacgag ccgatattga
    11161 tcgacatata gaacagggtg aatgcgccat caagccgcgg atctttcggc ggatagcact
    11221 tcgaaagcaa gctggctggg ttagctttaa acaggccgtt accgacagcg atagtcccca
    11281 gggcgatgaa aatcaggtca ggcttaagta gcgacatgcc ggtcatgaag tagccaatcg
    11341 ccagcacaag tgctccgaga acaatggtgc gtttggtccc cagcaggtgg tcgccgacat
    11401 agccgccaat ggaaatgagg ccatagacca gcgcagcaaa agcaccaaaa gtgacaaaag
    11461 cctgctcttg cgagaatcca agctgtttaa cgaagaaaac cgccagtacg ccctgcacgc
    11521 cgtagtagcc gaatcgctcc cataactcga caaaaaagat catgaaaaat gggcgaggtt
    11581 gctgcagcat ccccatgggt gttgttgtat tcatatttat taaccttcca ataccatcct
    11641 gaaaagtatg aacgcgtaag ggataacgcg cgaaaaagtt tacatctgtc agagattctt
    11701 aatattgatg cggtgaatag attaatacac tattacaaca gaaaataacc agctgatcac
    11761 actaatgatg gaaaatatga tggtgttaaa tgttgtattg gcaggagatt ttgattgcgt
    11821 acgcagatcg taggccagat aaggggttta cgctgcatca ggtaacaagg cgcgccaaaa
    11881 agcaaaacgc ccgcatatgc gggcgttcag gaacacgtcg tggggggatg attattcttc
    11941 ttcgtcgcgc agcggaacaa tcagcatatc aacgtgaacg gtgttgatca gctgacgtgc
    12001 ggaagacatc agtttgctcc agaagtcctg gtggtgacca caaaccacca gatccatatc
    12061 gtatttcttg attgcatcga ccagaacctg gcccaggtcg ccgctgccgc tcagggtttc
    12121 agtgattggg tagcctgcat tagtggaaag ctcggtcagc gcatgatgtg tctcttcaga
    12181 gatgcgtttc tgcatatcac ccagattcac atcaataagc ccggtgtata ggtcagagta
    12241 gtttacatct acgtggatca gagaaacttt cgcattgtag gggcgagcca tagagactgc
    12301 tttctctacc agaactttgc tttccgggga gagatcgact gcgatgagaa tgtgtttata
    12361 agccatagtg ttactccttc cataaagttg tcgatgactg gccagctagc gtttcttgtg
    12421 ccgttacgtc acccgcgtcc tgcgaactac gtgcgcgggg ctaacccagc cgtaaagatt
    12481 cagtgttaag accatcctta ccttatagcg acccggatga tccgtcaatc cgccttgctt
    12541 acccgttaga taaacaaatt ttcgacatga atatattgat agtggttaac cttctggaaa
    12601 aaaaacaacc tgatctccta cactatctat agagccgctc ggatgttggt caccacaaaa
    12661 gcggtttctc caggacctgg tgttaactga ttggcagcgt atcgggagcg gtagccccgg
    12721 ggtggaactc cgcgggcagg tcgccgggga ggaggtatga taagcaccgt cgcattattt
    12781 tgggctttat gtgtcgtttg cattgttaac atggcgcgct atttctcatc actacgcgcg
    12841 ttgttagtgg tactgcgtaa ctgcgatcca ttgctctatc aatatgttga tggagggggc
    12901 ttttttacct cacatggcca acccaacaaa caggtgcgtc tcgtttggta tatctatgcc
    12961 caacgttatc gcgatcatca cgatgatgaa tttattcgcc gctgtgagcg ggtgcgtcgg
    13021 cagtttattc tgaccagcgc attgtgtggt ctggtggtgg tcagcctgat tgcattgatg
    13081 atttggcatt aagcacttca gcaataataa cgcataaaaa aagcgggcca gtaaactgac
    13141 ccgctcgttt tggtgtgtac gattacagga actgcaagga gagccagtac agcccgccgg
    13201 aaagcagtac cgcagccgga agggtaaaca cccaggccat cagaatgctg gtcacggttt
    13261 tacgctgtaa gccgccacca tctaccacca tcgtccccgc gacagaagag gagagtacgt
    13321 gagtagtgga aaccggcatc ccggtataac tcgccaggcc gatagacact gccgccgtca
    13381 tctgggcaga catcccctga gcgtaggtca tgcctttctt accgattttc tcaccgatag
    13441 tcgttgccac acggcgccag ccaatcatcg taccgatacc taacgccagc gcgaccgcca
    13501 tgatgatcca caccggtgca tactcgatgg tgctaagcat gtcagacttc agtttcttca
    13561 acaggcgctg atcgtcagca ctcacgccag gcatcttcac cactttgtcg atagtgtcag
    13621 aaacgcacag cataatgcgg cgcatctggc tacgttgatc aagcgacagc ttgtcgtagc
    13681 tttccacatc ggtggtcaac atacctttca ggcggttgag cgcgttaatg gtattcgacg
    13741 gatggcagtg gaactccgca ggttgcgttg cgccagcttc cggagccggt actaactgat
    13801 cagcaccggt agcctgtttg agcagcgcag gatgctgctc aaagtaagct tcgacgttgt
    13861 tgatggcatc acgggtacgg gtgatttcgt agccagtggc attcatgttc accacgaagc
    13921 ctgctggcgc gacgccaatc aataccaaca taaccagacc aatgcctttc tgaccatcgt
    13981 tcgcgccgtg cgaaaacgcc acgccgatag cggaaaggat cagcgcgata cgcgtccaga
    14041 acggcggctt tttcttgccg tctttctttt cacgctccgc tggggtcagg tggatacggg
    14101 cgcgtttctt ggtgccgctc cagtagcgac gcagcaagaa aatcagaccg ccagcaaaca
    14161 ccaggccgac aataggggaa acgatcagag aaccgaaaat acttaatact ttcgggatat
    14221 tgagtgcatc caccactgac gtcccggtca tcaacgcatt ggttaaacca atcccgatga
    14281 tcgcgccaat cagcgtatga gagctggatg caggtaaacc aaagtaccag gtacccaggt
    14341 tccagataat cgccgccagc aacatagaga acaccatggc aaggccatga gacgatccca
    14401 tattaagcag cagatccgtc ggcagcatat gcacaatggc ataggcaaca ctcagaccac
    14461 ccagcaaaac acccaaaaag ttgaacaccg ccgccataac cacggcgagc tgagaacgca
    14521 tcgcgcgggt atagataacg gttgccacgg cgttggctgt gtcatggaaa ccattgatgg
    14581 cttcgtagaa cagcacaaaa gccagtgcaa gcaataataa cagcccggta tgcaaatcca
    14641 ggccagcaaa caaatgtagc ataggacgtt acgccatttt gaggacatga acgcggcgca
    14701 ttatcagtga ctttcgcggc gcgggcaaag tgaaatatag actttttttg atttgcctcc
    14761 tgtatggatt tcactcaaaa aataattatc ttatataatt caggcaaata cttcctttta
    14821 gtaatattga tgctggtgcg accactgagg aatctttaca attcacgccc gttttttcta
    14881 agaggagcgc aacgtggaaa ggtttgatgc cattattata ggcgctggtg cggcgggtat
    14941 gttctgttct gcgctggcag gtcaggcagg acgccgggtt ctgctgatcg ataatggtaa
    15001 aaaaccaggg cgcaaaatcc ttatgtctgg cggtgggcgc tgcaacttta ccaaccttta
    15061 tgtcgaacca ggcgcttatc tgagccagaa tccgcatttt tgtaagtctg cactcgcgcg
    15121 ttttacccag tgggatttca ttgatctggt caataaacac ggcatcgcct ggcacgagaa
    15181 aacgttagga caactcttct gcgatgactc cgcgcagcag attgtcgaca tgctggtgga
    15241 tgagtgcgag aagggcaatg taaccttcag attgcgtagc gaagtgctga gtgtggcgaa
    15301 ggatgaaaca ggcttcacgc ttgaactgaa cggcatgact gtcggttgcg aaaagctggt
    15361 catcgcgacc ggtgggctgt caatgccggg gctgggcgcg tcgccgtttg gttataagat
    15421 tgccgaacaa tttggcctca acgtgctgcc gacccgcgcg ggtctggtgc cattcactct
    15481 gcataaaccg ttgctcgaag agttacaggt gctggcgggc gtggcggtgc cttccgtgat
    15541 taccgctgaa aacggcatcg ttttccgtga gaacttactc ttcacccatc gcggcttgtc
    15601 tggaccggcg gtgttgcaga tttccagcta ctggcaaccg ggtgaatttg tcagtatcaa
    15661 tctgctaccg gatgtggatc tcgaaacctt cctgaatgag cagcgtaacg cacatccgaa
    15721 tcagagcctg aaaaacacac tggcggttca tctaccgaag cggttggttg aacgcttaca
    15781 gcaactcggg caaatcccgg atgtttcgct aaaacagctc aacgtgcgtg accaacaggc
    15841 actgattagc acattgaccg actggcgcgt acaacccaac ggcactgaag gctatcgcac
    15901 tgccgaagtg acgctcggcg gcgtggacac caacgaactc tcttcacgga cgatggaagc
    15961 gcgcaaagtg cctgggctgt acttcatcgg cgaagtgatg gacgttaccg gctggctggg
    16021 gggctataac ttccagtggg cgtggtcgag tgcgtgggct tgtgcgcagg atttgattgc
    16081 agcgaagtcg tcctgacctg ttacctaaag atgaaaatta ttagagccgc attaaaaatg
    16141 agctaatttt gatagtggct atcttgtgat tattttctaa tgagcccgtg aactgaaacc
    16201 ctccaggctt aatataaggt ggaaggaaag gtgattgaaa actcacccag tggccagatc
    16261 tttataaaat atgaacaggt tgaaaaaaac agtaactttg ttgttttttt atccttaacc
    16321 actatgcatt aatgctgcgt tatttcatga tgcctaagaa aaaccagcgt tacgcaaatg
    16381 gtcaacgctg gttttatccg gtacgttgca attatttttt agcagaacct gcttctaata
    16441 ttgaaacgat tgagaacaac gtaaagcata ccgctcctaa accgaccaaa acccgcgaag
    16501 gaataaaata tcccatgtcg gtctgcgcca tttccgcaag aaacgatgcc aggaaaaggc
    16561 aaaacaggca ggtgaagacg ggaatcatcg gtattctgtt ggctaacgaa caggtacgtc
    16621 tccataccag tgccagtagc cagacttttg agaatatgct gtaacagatc attccgaggc
    16681 aaataagaat aatgccggga gccagtcggg cgcttgcatc ggaactgact aagacgtata
    16741 tcccctgcag taccgtgatt gagccgagaa aaataaccca ataacaccac agccagtgtt
    16801 ctttagttga aaacgtatta cgtgtttgat ggacaattgt ggcaacaagg ccaattagac
    16861 aggcacagat tgcggttaac ccaagcaata cgtgacccgc gacataatgc ggagttatgt
    16921 cagcactacg taacagcgta atagaccaaa tgaaaccgag cagggtgagt aaaacgggaa
    16981 cggcaatgag gcaattaccg attaatgaag aataagcctg taccggcgtt ccatcgctct
    17041 tgctccccgc tgcatttttg ggaatgagca gaaagtgacc ggatgacgct gccaccgtcg
    17101 atacacaggc ggcaatcata ccaacgccga aaataacatg gccggcgaca aactcgtctg
    17161 ccatcacatc attgcctgct aacagtgccc aaccccatat catggtgata attgaccccg
    17221 catagccaat aatggggaac aatgtattgt aaaacgtatt aacgccgcgc gtgagctgcg
    17281 aaatgataat aaatgcggta gtgaataatg ccaggcatat ggccgccaga gaaatcagca
    17341 catgacccgc gacgaaatac tcgctttgcc ctgaactttc cagcacaaag ccacctaacg
    17401 cgatgcaaat taatcccata atcaaaggga ttaatttgaa aagccacccg atatatatgt
    17461 tcactttaaa ctcctcaggt atccctgcta ataatgaaat gtgcattaat gataatttca
    17521 gcctgttaat ataacgacga gatatcatta aagacaaatg catagcataa ctatagcggg
    17581 gtttactgtg ccgagtgcca ttttttaccg gttaaaaggc catgataaa
//
LOCUS       DAPHDW010000092        17567 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.093, whole genome shotgun sequence.
ACCESSION   DAPHDW010000092 DAPHDW010000000
VERSION     DAPHDW010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17567)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17567)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17567
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.093"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            86..1225
                     /gene="arnB"
                     /locus_tag="RH057_003043"
     CDS             86..1225
                     /gene="arnB"
                     /locus_tag="RH057_003043"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HDV3129379.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWLTTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLAKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            1229..2197
                     /gene="arnC"
                     /locus_tag="RH057_003044"
     CDS             1229..2197
                     /gene="arnC"
                     /locus_tag="RH057_003044"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HDV3129380.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILLNRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHSEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            2197..4179
                     /gene="arnA"
                     /locus_tag="RH057_003045"
     CDS             2197..4179
                     /gene="arnA"
                     /locus_tag="RH057_003045"
                     /EC_number="1.1.1.305"
                     /EC_number="2.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
                     /protein_id="HDV3129381.1"
                     /translation="MKTVVFAYHDMGCLGIEALLAAGYEISAIFTHTDNPGEKAFYGS
                     VARLAAERGIPVYAPDDVNHPLWVERIAQLSPDVIFSFYYRHLICDEILQLAPAGAFN
                     LHGSLLPKYRGRAPLNWVLVNGETETGVTLHRMVKRADAGAIVAQLRVAIAPDDIAIT
                     LHHKLCHAARQLLEQTLPAIKHGNILEIAQRENEATCFGRRTPDDSFLEWHKPASVLH
                     NMVRAVADPWPGAFSYVGNQKFTVWSSRVHPHASKAQPGSVISIAPLLIACGDGALEI
                     VTGQAGDGITMQGSQLAQTLGLVQGSRLNSQPACTARRRTRVLILGVNGFIGNHLTER
                     LLREDHYEVYGLDIGSDAISRFLNHPHFHFVEGDISIHSEWIEYHVKKCDVVLPLVAI
                     ATPIEYTRNPLRVFELDFEENLRIIRYCVKYRKRIIFPSTSEVYGMCSDKYFDEDHSN
                     LIVGPVNKPRWIYSVSKQLLDRVIWAYGEKEGLQFTLFRPFNWMGPRLDNLNAARIGS
                     SRAITQLILNLVEGSPIKLIDGGKQKRCFTDIRDGIEALYRIIENAGNRCDGEIINIG
                     NPENEASIEELGEMLLASFEKHPLRHHFPPFAGFRVVESSSYYGKGYQDVEHRKPSIR
                     NAHRCLDWEPKIDMQETIDETLDFFLRTVDLTDKPS"
     gene            4176..5066
                     /locus_tag="RH057_003046"
     CDS             4176..5066
                     /locus_tag="RH057_003046"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HDV3129382.1"
                     /translation="MTKVGLRIDVDTFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAMPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            5066..6718
                     /gene="arnT"
                     /locus_tag="RH057_003047"
     CDS             5066..6718
                     /gene="arnT"
                     /locus_tag="RH057_003047"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HDV3129383.1"
                     /translation="MKSVRYLIGLFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFASLAMLMAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVVSPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWPFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIDRQERLVLIQYRPK"
     gene            6715..7050
                     /gene="arnE"
                     /locus_tag="RH057_003048"
     CDS             6715..7050
                     /gene="arnE"
                     /locus_tag="RH057_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HDV3129384.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMVLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            7050..7436
                     /gene="arnF"
                     /locus_tag="RH057_003049"
     CDS             7050..7436
                     /gene="arnF"
                     /locus_tag="RH057_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HDV3129385.1"
                     /translation="MGLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(7430..7696)
                     /gene="pmrD"
                     /locus_tag="RH057_003050"
     CDS             complement(7430..7696)
                     /gene="pmrD"
                     /locus_tag="RH057_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HDV3129386.1"
                     /translation="MEWLVKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKVGD
                     LLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(7806..9161)
                     /gene="menE"
                     /locus_tag="RH057_003051"
     CDS             complement(7806..9161)
                     /gene="menE"
                     /locus_tag="RH057_003051"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HDV3129387.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELASGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQRVEGAHAATWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAEGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGSPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVPVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(9158..10120)
                     /gene="menC"
                     /locus_tag="RH057_003052"
     CDS             complement(9158..10120)
                     /gene="menC"
                     /locus_tag="RH057_003052"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HDV3129388.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQETWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELADTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFVRETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSTLPVVEVDALERLL"
     gene            complement(10120..10977)
                     /gene="menB"
                     /locus_tag="RH057_003053"
     CDS             complement(10120..10977)
                     /gene="menB"
                     /locus_tag="RH057_003053"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HDV3129389.1"
                     /translation="MIYPDEAMLYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(10992..11750)
                     /gene="menH"
                     /locus_tag="RH057_003054"
     CDS             complement(10992..11750)
                     /gene="menH"
                     /locus_tag="RH057_003054"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HDV3129390.1"
                     /translation="MILHAQAKHGKPGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLPGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFCTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(11747..13417)
                     /gene="menD"
                     /locus_tag="RH057_003055"
     CDS             complement(11747..13417)
                     /gene="menD"
                     /locus_tag="RH057_003055"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HDV3129391.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     STFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRICDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HRPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(13506..14801)
                     /gene="menF"
                     /locus_tag="RH057_003056"
     CDS             complement(13506..14801)
                     /gene="menF"
                     /locus_tag="RH057_003056"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HDV3129392.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANNPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLQPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(14880..15185)
                     /gene="elaB"
                     /locus_tag="RH057_003057"
     CDS             complement(14880..15185)
                     /gene="elaB"
                     /locus_tag="RH057_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HDV3129393.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKARAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(15240..15701)
                     /locus_tag="RH057_003058"
     CDS             complement(15240..15701)
                     /locus_tag="RH057_003058"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3129394.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFISVTEVYEEDGIPHIGMAREVIQA"
     gene            15766..16683
                     /gene="rbn"
                     /locus_tag="RH057_003059"
     CDS             15766..16683
                     /gene="rbn"
                     /locus_tag="RH057_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HDV3129395.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
     gene            16871..17527
                     /locus_tag="RH057_003060"
                     /pseudo
     CDS             16871..17527
                     /locus_tag="RH057_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416772.1"
                     /note="ElaD; specific cysteine protease which targets
                     ubiquitin and ubiquitin-like proteins covalently bound to
                     target proteins; SseL in Salmonella is required for
                     macrophage killing and virulence, and it is secreted by
                     the salmonella pathogenicity island 2 type III secretion
                     system; incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deubiquitinase"
BASE COUNT         4145 a         4733 c         4422 g         4267 t
ORIGIN      
        1 aatttcgctc caaatggcaa aatatacaca acactcttta tagcaaatat aagtggacag
       61 gtattcaatg gcggaaggaa aagcaatgtc agaatttttg cctttttcgc gaccagcaat
      121 gggcgtggag gaactcgctg cagttaaaga ggttctcgaa tccggttggc tcacaaccgg
      181 tccgaagaat caggcgcttg agcaagcctt ttgccagttg acgggaaatc agcatgccat
      241 cgcggtcagt tcagccaccg ccggaatgca tatcacgcta atggcgttgg aaattggcaa
      301 gggcgatgaa gtgattacgc cttccctgac ctgggtttca accctcaata tgatttcctt
      361 gttgggtgca acgccggtaa tggtggatgt cgaccgcgat acgctgatgg tcacgcctga
      421 agcgatcgag tcagccataa cgccacgcac taaagccatc attccggtgc attatgccgg
      481 tgcgccagca gatattgacg ccattcgcgc cattggcgaa cgttacggca tcgcagttat
      541 cgaagatgct gcccatgccg tcggtacgta ttacaaaggg cgacatattg gcgcaaaagg
      601 taccgctatt ttttcatttc atgccattaa aaatattacc tgtgcggaag gtggcctgat
      661 tgtaactgat aatgaaaacc ttgcccgcca gctacggatg ctgaaatttc acggtctggg
      721 tgtcgatgcc tatgacagac aaacctgggg ccgtgcaccg caggctgaag tcttaacacc
      781 gggctataag tacaatctga ccgatattaa cgccgcaatt gccctgacac agttagcaaa
      841 attagagcac ctcaacaccc gtcggcgcga aattgcccag caatatcagc aagcactggc
      901 agctctcccc tttcagccat taagccttcc cgcctggccg cacgttcacg cctggcatct
      961 gtttattatt cgtgtcgatg aacaacgttg tggtatcagt cgcgatgcgt tgatggaagc
     1021 gttaaaagaa agaggcattg gtaccgggtt acatttccgc gccgctcaca cacaaaaata
     1081 ttatcgcgag cgttttccca cgctgtcgtt accgaatacc gaatggaata gcgaacgcat
     1141 ctgttctttg ccgctgttcc cggatatgac taccgccgat gccgaccgtg tcatcacagc
     1201 ccttcagcaa ctcgcaggac aataagccat gtttgaaatc caccctgtta agaaagtctc
     1261 ggtggttatt cccgtttata acgagcagga aagcttaccg gaattaatca ggcgcaccac
     1321 cacagcctgt gaatcgttgg ggaaagagta tgagatcctg ctgattgatg acggcagtag
     1381 cgataattcc gcgcatatgc tggtcgaagc ctcacaagcg gagaacagcc atattgtgtc
     1441 tattttgctt aaccgcaatt acgggcaaca ttcagcgatt atggcgggat tcagtcacgt
     1501 taccggcgac ttaattatta cccttgatgc cgatctccag aatccgccag aagaaatccc
     1561 ccgcctggtg gcaaaagccg atgaaggtta cgacgtggta gggactgtac gccagaaccg
     1621 ccaggacagc tggtttcgta aaacagcttc gaagatgatt aaccggctta ttcagcgcac
     1681 caccggcaaa gcgatgggtg actacggttg tatgctgcgc gcctatcgcc gtcatattgt
     1741 cgatgcgatg ttgcactgcc atgaacgtag cacctttatc ccgattctgg cgaatatctt
     1801 cgcccgccgt gccattgaaa ttccagtaca tcattccgag cgtgagtttg gtgaatccaa
     1861 atacagtttt atgcgcctga ttaatttgat gtacgacctg gtgacctgcc ttaccacaac
     1921 gccgctacgt atgctgagtc tgctcggcag cattattgcg attggaggtt ttagcattgc
     1981 ggtgctgctg gtgattttac gcctgacctt cggaccacaa tgggcggcag aaggcgtctt
     2041 tatgctattt gccgtgctgt ttacttttat tggcgctcag tttatcggca tgggattact
     2101 cggtgaatat atcggcagga tctacaccga tgtccgcgcc cgcccccgct attttgttca
     2161 gcaagttatc cgtccatcca gcaaggaaaa tgaataatga aaaccgtcgt ttttgcctac
     2221 cacgatatgg gatgcctcgg tattgaagct ctgctggctg ccggttacga aattagcgcc
     2281 atttttaccc ataccgataa tcccggtgaa aaagcctttt atggttcggt ggctcgtctg
     2341 gcagcggaaa gaggcattcc ggtttatgcg ccggatgacg ttaatcatcc gctgtgggtg
     2401 gaacgcattg cccaactgtc gccagatgtg attttctctt tttattatcg ccatcttatt
     2461 tgcgacgaaa ttttgcagct cgctcccgca ggtgcattta atctgcatgg ttcgctgtta
     2521 ccaaaatatc gtggtcgcgc gccgctgaac tgggtgctgg tgaacggtga aacggaaacg
     2581 ggcgttacat tgcaccgaat ggtaaaacgt gccgatgctg gggccattgt ggcgcaactg
     2641 cgcgttgcca ttgcgccaga cgatatcgct attacgctgc atcataaatt gtgccatgcc
     2701 gcgcgccagc tactggaaca gacattaccc gccattaaac acggtaatat tctggaaatc
     2761 gcccagcgcg aaaacgaagc cacctgtttt ggtcgcagaa cgccggatga cagtttcctt
     2821 gaatggcata aaccggcatc cgtactgcac aacatggtac gtgccgttgc cgatccgtgg
     2881 ccgggtgcct tcagctatgt tggcaatcag aaatttaccg tctggtcgtc gcgtgttcat
     2941 cctcatgcca gcaaagcaca gccggggagc gtgatttcta ttgcaccgct gttgattgcc
     3001 tgtggcgatg gcgcgctgga aatcgtcacc ggacaggcgg gcgacggcat tactatgcag
     3061 ggctcgcaat tagcgcagac gctgggcctg gtgcaaggtt cacgcttgaa tagccagcct
     3121 gcctgcaccg cccgacgccg tacccgggta ctcatcctcg gggtaaatgg ctttattggc
     3181 aaccatctga cagaacgcct gctgcgcgaa gatcattatg aagtttacgg tctggatatt
     3241 ggcagcgatg cgataagccg ttttctgaat catccgcatt ttcactttgt cgaaggcgat
     3301 atcagtattc attccgaatg gattgagtat cacgtcaaaa aatgtgatgt cgtcttgccg
     3361 ctggtggcga tagccacgcc gattgaatat acccgcaacc cgctgcgcgt atttgaactc
     3421 gattttgaag agaatctgcg cattatccgc tactgcgtga agtaccgtaa gcgaatcatc
     3481 ttcccgtcaa cttcagaagt ttatgggatg tgtagcgata aatacttcga tgaggaccat
     3541 tctaatttaa tcgtcggccc ggtgaataaa ccacgctgga tttattcggt atcaaaacaa
     3601 ttacttgatc gggtgatctg ggcctatggc gaaaaagagg gtttacagtt caccctcttc
     3661 cgcccgttta actggatggg accgcgactg gataacctta atgcggcacg aatcggcagc
     3721 tcccgcgcta ttacgcaact cattctcaat ctggtagaag gttcaccgat taagctgatt
     3781 gatggcggaa aacaaaaacg ctgctttact gatattcgcg atggtatcga ggcgttatac
     3841 cgcattatcg aaaatgcggg aaatcgctgc gacggcgaaa ttatcaacat tggcaatcct
     3901 gagaacgaag cgagcattga ggaactgggc gagatgctgc tggcgagctt cgaaaaacat
     3961 ccgctgcgcc atcatttccc accgtttgcg ggctttcgcg ttgtcgaaag tagcagctac
     4021 tacggcaaag gatatcagga cgtagagcat cgtaaaccga gcatccgcaa tgcccaccgc
     4081 tgcctggact gggagccgaa aattgatatg caggaaacca tcgacgaaac gctggatttc
     4141 ttcctgcgca ccgttgatct tacggataaa ccatcatgac caaagtaggc ttacgcattg
     4201 atgtcgatac ctttcgtggc acccgtgaag gcgtgccgcg tctgctggaa atcttgagta
     4261 agcataatat tcaggccagc atttttttca gcgtcggccc ggacaatatg ggccgccatc
     4321 tctggcgact ggtgaagcca cagtttttgt ggaagatgct gcgctcaaat gcggcatcgc
     4381 tttatggctg ggatatttta ctggcaggta cggcctggcc aggtaaagag attggtcatg
     4441 ccaatgccga tatcattcgt gaagcggcta aacatcacga agtcggcctg cacgcctggg
     4501 atcaccatgc ctggcaagcc cgtagcggta actgggatcg gcaaacaatg atcgacgata
     4561 ttgcacgcgg tcttcgcact ctggaagaga ttatcggtca accggtaacc tgttctgccg
     4621 ctgcgggctg gcgtgccgac cagaaggtga tcgaagcaaa agaagcgttc catttgcgct
     4681 acaacagcga ttgtcgtggg gccatgccgt tccgtccatt gctcgaatca ggaaaccctg
     4741 gcactgcgca aattccggtg accttaccca cctgggatga agtgattggt cgggatgtga
     4801 aagcagaaga ttttaacggt tggttactca accgcatcct gcgagataaa ggcacgccgg
     4861 tttataccat tcatgcagaa gttgaaggct gcgcttatca gcataatttt gtggatctcc
     4921 tcaaacgcgc agctcaggaa ggcgtgacat tttgcccttt aagcgaactg ttatcagaga
     4981 cgttgccgct cggacaagtt gttcgcggaa atattgccgg acgtgaaggc tggctgggtt
     5041 gccaacaaat tgcgggtagt cgctgatgaa atcggtacgt taccttatcg gcctcttcgc
     5101 gtttattgcc tgctattacc tgttaccgat cagcacgcgt ctgctctggc aacccgatga
     5161 aacgcgttat gcggaaatca gtcgagaaat gctggcatcc ggcgactgga ttgtgcccca
     5221 tctgttaggg ctacgttatt tcgaaaaacc cattgccgga tactggatta acagcattgg
     5281 gcaatggcta tttggcgcga ataactttgg tgtgcgggca ggcgttatct ttgcgaccct
     5341 gttaactgcc gcgctggtga cctggtttac tctgcgctta tggcgcgata aacgtctggc
     5401 tctactcgcc acagtaattt atctctcatt gtttattgtc tatgccatcg gcacttatgc
     5461 cgtgctcgat ccgtttattg cattctggct ggtggcggga atgtgcagct tctggctggc
     5521 aatgcaggca cagacgtgga aaggcaaaag cgcaggattt ttactgctgg gaatcacctg
     5581 cggcatgggg gtgatgacca aaggttttct cgcccttgcc gtgccggtat taagcgtgct
     5641 gccatgggta gcgacgcaaa aacgctggaa agatctcttt atttacggct ggctggcggt
     5701 tatcagttgc gtactgacgg ttctcccttg gggactggcg atagcgcagc gggagcctga
     5761 cttctggcac tattttttct gggttgagca tattcaacgc tttgcactgg atgatgccca
     5821 acatagagct ccgttctggt actacgtgcc ggtcatcatt gccggtagcc tgccgtggct
     5881 gggattactc cccggtgcac tgtacacagg ctggaaaaac cgcaagcatt ccgcaaccgt
     5941 ctatttgttg agctggacga taatgccgct gctgtttttc tccgtcgcta aaggtaaatt
     6001 gcccacctat attctttcct gctttgcatc tctggcaatg ctgatggcgc attacgcttt
     6061 gctggcagca aaaaataatc ctctggcgct gcggattaat ggctggatta acatcgcttt
     6121 tggcgtcact ggcattattg ccacatttgt ggtctccccg tggggaccaa tgaacacgcc
     6181 ggtgtggcaa accttcgaga gctataaagt cttttgtgcc tggtcgattt tttcgctatg
     6241 ggcatttttc ggctggtaca ccttaacaaa cgtcgaaaag acctggcctt ttgccgcgct
     6301 ttgcccgctg gggctggcgt tgctggtagg attttcaatt cctgacagag ttatggaagg
     6361 aaaacatccg caattttttg tcgagatgac acaagaatca cttcagccaa gccgctatat
     6421 tcttactgat agcgtcggtg ttgccgcagg tctggcatgg agcctgcaac gcgatgacat
     6481 catcatgtat cgccagacag gtgagttgaa atacggcctt aattatccgg atgcgaaagg
     6541 gagatttgtc agcggtgatg agttcgcaaa ctggcttaat caacatcgtc aggaggggat
     6601 tattactctc gtgctttcgg ttgaccgcga tgaagatatc aacagtctcg ccattccgcc
     6661 cgcagatgcc atcgatcgtc aggagcgtct ggtgctgatt cagtatcgtc ccaaatgatc
     6721 tggctaacat tagtctttgc cagcttgctt agcgttgccg ggcagttgtg tcagaaacag
     6781 gcaacctgct ttgtggcgat aaacaaacgg cgcaaacata tcgtgctgtg gctgggactg
     6841 gcgctggctt gtcttggtct tgccatggtg ctctggctgc tggtcttgca gaacgtaccg
     6901 gtaggcattg cctacccgat gttaagtctg aattttgtct gggtgacgct ggctgcagta
     6961 aaactgtggc acgaaccggt atcgccgcgt cactggtgtg gggtggcgtt cattattggt
     7021 ggcattgtga tcctcgggag tacggtgtaa tgggcctgat atggggatta ttcagcgtca
     7081 ttattgcctc agttgcgcaa ttaagcctgg gttttgcggc aagtcatctg ccgccgatga
     7141 cgcacttatg ggattttatc gcggctctgt tagcttttgg cctggatgcc cggatactgt
     7201 tactcgggct gctgggttat ctgctttccg ttttttgttg gtataaaacg ctacataaac
     7261 ttgcgctaag caaagcctat gccttgctca gtatgagtta tgtgctggta tggattgctt
     7321 caatggtact tcccggctgg gaagggactt tttcgctgaa agcactactg ggagtagcct
     7381 gtattatgag cgggttgatg ctgattttcc tgcccacgac aaaacaacgt tactgagttt
     7441 tccctgccgc tgtacactgc cgttcccact catcggggct ataactgctt gccgagagga
     7501 ctttcaccgt atgcagcttt tcacgattaa tacaataaag cgcattctgc aaaggcgaga
     7561 gtaaatcccc cactttcacg gcgaagtcac tcttcacttc ggcgatcatt tttatcgcgc
     7621 cgccagcatc gcagagcata agtacatgtc tgttatcttg tttattgcag cacgattttt
     7681 tgaccagcca ttccattgca ttatcctgtt tgctaagagt tttcacatca atttacagat
     7741 taacagataa actctttatc aacggcaatg gaatggcaga aatgagaagt gaaacggagg
     7801 gttaattatt gctgacgttg cacccactct tttagcgcct gacgtgaaat tttaataccg
     7861 ccgtttttca gctccggcgg taaagtcagc cagcgcaccg gttgttgaaa acgagccagc
     7921 ttatctttca cccattcgcc aagatcaacg gtttgctggt catactccac caccgctacc
     7981 ggccgatgac caaactcctt gtcggcaacg ggaacgataa acacctgcaa aaccgcagga
     8041 tgtgcagcaa taacacgctc gacttcttcc ggctgaatac cctcaccgcc actgaagaac
     8101 agattgtcta aacgtccgac aatggtcagc ttgccattat gcatctcacc gcgatcgcgc
     8161 gtagcgtacc agccttcgtc attaaccagt gaaaccagtt gcccgttacg ccagtaacct
     8221 tctgccatac tggcagcccg cagccacact tcattattaa cgattttcac ttcccgaccc
     8281 ggcagcggcg aaccaacgtc tgccaggccg tcggcttctt tcgcacacac cgtggaggca
     8341 aactcggtca gaccatagcc gcaaaagcaa cgaatcccct gctcgcgcgc ctgttccgtc
     8401 aactcgaccg ggatagccgc gccgccaagt aacaccgctt tcagggaaac ggaactgcgg
     8461 ttaaccagca aacgccagag ttgtgttggc accagtgaag cgtgagtaca gcctgccagc
     8521 atttgctcca atggctgttt atcacgcacc gtcatccgcg caccagcgta taaccagcgc
     8581 cacataattc cctgaccgga gacgtgaaac agcggtaaag agagcaacca gtcatcgtga
     8641 tcgccaaacg gaatcaacga taacacccct tcggcactgg caagatgggc ctgataagta
     8701 tggacagcgg ctttcggcaa cccggtagaa cccgaggtca acgtcattga gcacagacgc
     8761 gtcggctgcc acgtagcggc atgtgcgcct tcaacccgct gaatgtgcag cgacgttaac
     8821 gccggaaacg tgttttcccc atccggcacc agcgcaaatt gcagcgtcag attgggcagc
     8881 aattcttcaa gcaacggttg cggcagctga gggttcacgg gcaacacccg cgccccgcat
     8941 tgcagtaacg ccagccaggc gagcagcgtt tgcggcgtat tccacgcccg caacatcacg
     9001 ccgctgccct caacaacgcc ctgcgccgca aagcctgagg ctaattcatc gacgcgagca
     9061 caaagctcgc gccagttaag ttgctcgtca ttaagacgta aggcgatggc ttctccccgc
     9121 acttgccgcc agtgacgcca cggccagtca gagaagatca taacaaccgc tccagtgcat
     9181 caacttccac gacaggcagc gtgctacccg gccagcgacg tacctgctgc gcctgcatca
     9241 gatccagcgt gtccagccct ggaatggtgt ccggcgttaa ccaggcggca atccgcgcca
     9301 gttgcgttaa gcctaagctc gattcaatgg aagaactgat caccgccgtc agccccagcg
     9361 cgtgcgccgc ctgtacctgc tcgcgtactt tctccagact gcccgtgagc gtgggtttga
     9421 taactaccgc gcgcacgccc tcttcagcca caaaggcaaa atccggctcg cgcaagcttt
     9481 catcccaggc aatggcaatg ccggtttcac ggacaaacgc tcgcgaatca tcgcgggttt
     9541 tgcacggctc ttcgagaaac gcgatgcggt ggcgataatc cgggttaaca tatttggcaa
     9601 actgctggcc tttcagcggt gtccaggcgc gatttgcgtc aagacgcaaa tgcagatccg
     9661 gaattgcctc cagcaacaga ttaaccacca tgccgtcgcg cacggcttcg tacaatccca
     9721 ctttgacctt cgccactttc tcgcctggca tatcagcaag tttgaggatc agatcgtcag
     9781 gatcgccatt acacagtggt gccgcacggt aattggccgc ctgcgggagc gtatctgcca
     9841 gctccgccaa tgcacagctt acgccaaagg caacggaagg catctgcggt agctcgcaat
     9901 cgcctgccag ccagttattt acccaggcaa gcagcacgct ttgcgcctct tcccaggttt
     9961 cctggctgaa gcccggcagt ggggagatct ccccccaccc ttcgcgctcg ccctcacgca
    10021 ggcaaacgta cagcccgtcg cgggttttta accgcctgtc gcgcagaacc acccccgcgt
    10081 ccatggggat ctgccagcgg tatacctgcg cgctacgcat tacggattcc gtttaaattt
    10141 gctgaaatca ggctgacgtt tctggttgaa agcgttgcga ccttcctgac cttcttccgt
    10201 catgtagaac agcatggtgg cgttgcccgc cagctcctgc agccccgcct gcccgtcaca
    10261 gtcggcgttc agcgcagctt tcaggcagcg cagcgccatc gggctgtttt gcagcatttc
    10321 gcggcaccaa cggacggttt ctttttccag atccgccagc ggtaccacgg tgttgacaag
    10381 gcccatatcc agcgcctgtt ttgcgtcgta ctgacggcac aggaaccaga tttctcgcgc
    10441 ttttttctgc ccgacgatac gagccatgta ggaagcgccc cagccgccgt cgaaggaacc
    10501 gactttcggg ccagtctgac cgaagatggc attatctgct gcgatagtca ggtcgcacat
    10561 catgtgcaga acgtgaccac cgccgatgga gtagccagcc accatcgcga caaccggttt
    10621 cggacaggta cggatctgac gctggaagtc cagcacgttc aagtgatgta cgccggaatc
    10681 atctttatag ccgccgtaat caccacgcac tttctggtca ccaccggagc agaacgcttt
    10741 atcgcctgcg ccagtcagaa tgatcacgcc gatattgtcg tcataacgcg catctgccag
    10801 tgcctggatc atctctttta ccgtcagagg acggaaggca ttgcgcacct gcggacgatt
    10861 aatggtgatt tttgcgatac cgtcggtgga tttttcataa cgaatgtcct cgaaaccttc
    10921 ggagcagtcg tgccattcaa ccggtgcgta aagcattgct tcatcaggat aaatcatatt
    10981 gtgtccttta gtcagaaacg caagatctgc gccagacttg cgattacgcc agcgggattt
    11041 tcccgatgcg cgttatgtcc ggcgcgagga atgacatggc agtcggcagc cagttccgcc
    11101 gccagggcgc ggaatttgct gtcacgttca ccacataaat aataaaacgc aaatgtgcgg
    11161 gcactaaggt tagcacgtaa atcaggctgg acggcgagag aagtcgcctc cagcatggcg
    11221 gcaagggttg cgccattatt gttgctgcgc agcgccacca gctcccggcg ttgatcgtca
    11281 ttgagcgagg caaaaacagg ctgttgatac cagtcggcaa ataccgccgt taacggttct
    11341 gtgcaaaaac gctgcgccca ttggcgatcg gaacgctgac gttccgcacg ttgttcagca
    11401 ttttgcagcc ccggatgccc gccttcgaca ataaccccac aaagccccgc cagcccctgg
    11461 caagccgcca tcatcgccac ccgtccgcca agcgagtacc ccaccagcca gaagtcaagg
    11521 atgttgtaac taaccaaggt tttacgcagt aagtcggtga catcatcaaa tccatcgacg
    11581 ctaatcgccg ccgaaccacc atgacctggg agatcaacat acaaccgtga gtagtcggca
    11641 aacgcctcgc ccacttcttg ccattcgtgg caatcgccgg aaaaaccgtg caaaaacacc
    11701 agccagggta aacctggttt tccgtgtttt gcctgcgcgt gcaggatcat aaatggctta
    11761 cctgcgccag aagttgctgg agcgtttgcg caccatcggt gtcgttaacc accatttcaa
    11821 tcaccgtggt ggttggcgtg cgccaggcgt cggcaaatgc cgtttcaagt tcctgccagt
    11881 tttgcggacg atgatatttc agctcgaaca tcgcggcggc gtgctcaaaa tggacgtttt
    11941 gcggcatcag atagaaacgc tcacgctcgc tttgcggcgt tggcaacagc gagaaaattt
    12001 gcccgccgtt gttgttcacc acaattaata ccagcggcgc agaaacctga cgcaataacg
    12061 ccagcgcgtt gagatcgtaa agtgcggaga gatcgcccac aatcgccagc gtcggtttgc
    12121 cgcttgcccg ctgaacgcct gcggcggtcg aaagcagtcc gtcgataccg ctggcccctc
    12181 ggttgctgta caccgggtaa cctgccggaa gttgcgaaag cgcatcaatc agacgtacca
    12241 ccaggctgtt accaacaaac aattgcccct gttcaggcag atagtcgcag atgcgatgcg
    12301 ccagttgcgc ttcgccaaac gcatcacggc gggcaataac cgcctgcatt gcctgttccg
    12361 ccaggcgcgg gatttcaacg caccagggct ggcgtttttc tgccggatgc agctccagcc
    12421 agtcggcaat attggcaatt aagcgacgtc cgcgatggtg tgccggatca agtcgccctt
    12481 caatgtcatc aacaatccag tactcttctg gttcacagct tgcctgccat tgcaggagcc
    12541 gtttccccgt caggctgctt cccagttgca ccacaatttg cgcctgctgc agctcgctgg
    12601 tcgctttggc attgcctaac caaagatcgg cacacggcag cggctgcccg gtttgtgaca
    12661 gcacatcgcc aatcagcggc cagccaagag tttgcgccca cagggcaact tttttgccct
    12721 cttccgcact catgcgcccg gcaaccacca cgccgcgctt ttgtcgccag aagaaccagt
    12781 cgcgctgttt ttcactttcc agacgaggcg cttcacgcag ccacggtttg tcgtcctgcc
    12841 accagtcacc cagacgctgt tgccagctaa gcccggtatc gtccatttcg ccatacagcg
    12901 gttcagcaaa cgggcagttg atatggactc cccccgcatg aagcgtaccg agagcgtggt
    12961 cgatggtaga aaccagccaa cgtgcgggga tatcctgggt cgggcgcggc aatgaaatac
    13021 tgtgcgtggg gtgagaggcg aacattcccg gctggcgaat tgcctgattc gcgccgcagt
    13081 caattagctc cggcgggcga tcggcggtta acaggatcag tttttctccg gttaacccag
    13141 cttcaatcag tgccggatag agatttgcta ccgccgtgcc ggaggtcaca atcaccgcca
    13201 ccggctgctt gctgactttc gccagcccca gcgccagatg tcccagtcca cgctcatcga
    13261 aatgggtgtg gtgaatgaat gtggaattct ccgccgccgc taacgttaac ggtgtagaac
    13321 gcgagccagg ggcgatacag atgtgtctga cgccgtgacg cgttaatgct tccagaatga
    13381 ccgccgccca gcgtcggtta aatgcgctta ctgacatgag tttgtccggt atcaatattg
    13441 cggctaagta taaggagcta caaaaataga ttattgatat gaatcggtaa tgatgcgact
    13501 cattactatt ccatctgtaa taaagtacgc agccctgccg ctttgttgtc gatttcctgc
    13561 cactcttgct cggggtcgga accacggaca atgcccgcgc cagcatataa tcgcacgaca
    13621 ttgccgctaa tttttgctga gcgcagggaa acgcagaatt cgctttgttg taatgagaga
    13681 tagcccgcag aaccggcgta ccattctcgg gtgaacggtt cgtgacgggc gataaactgt
    13741 cgcgccagat cgcgcggtaa gccagcaact gctgccgttg gctgcaactg atgtaaacag
    13801 atcacatcat ccgctttgtt gagtgaagtc cagatacagc ggcgaagatg ctgcacttta
    13861 cgcagacgca gtacctgcgg cggtaaaaca tccagcgtct gggtatcggc ctgtaatcgt
    13921 tggcagatat cttccaccac cagcatgttc tcgcgctggt ttttatcatc cgccatcagc
    13981 cactctccta actgctgcgc ctgcttatca tcaggattat ttgctactgt tcccgccagc
    14041 gcttcagtac gcagcgcttt gtcacgccgc cgccataacc gttccggtga agagccaaga
    14101 aaagcatttt cgccatcaaa ggccatgtaa aaatggtagc aattcagatt cagtcgacga
    14161 ctggcagcca tcatcgccgc cgcgttgacc ggacttgcga aatgcaggtc agttgcccga
    14221 gcgagcacca ctttgtcgag ctcaccttcg gcgatggttt tcgttgccag ttcgattaat
    14281 tgcgtccagc ccgttttgtc cggccagtgt tgttctcgcg tggtggttaa atgtaaccca
    14341 ggcaagggct tgatactcac cagtgtggcg ataaattctt ttgcctgaat cgcatcgtgc
    14401 tgaagggagc tttcgctgaa tagcgtcagc cgcagcgtgg ctttaccgcc acagcgtcgc
    14461 cattccaggc ggggtaaaag taaattgccc tgcgacgggt caaaagcatt cagcccccaa
    14521 atgcgtaagt cggcgtgttc cgggtgctgg cgaagaaaac gttgtgcctg gtccaacgac
    14581 gtaaaacggg taatcgcgcc caggacggca gcttcttcat caccattacg ttgttgccag
    14641 tagaattgcg ggtacgtttg ctgactggcc agccagctca aggcatcaaa agcgtctttg
    14701 agagggaaag gaatatcgat aacccgaatg ccgggtgtcg ccggaatctc ttgcgacaaa
    14761 tggcgcagta gattttccag cgccgtagta agtgattgca cgcggacctc tccctgttaa
    14821 aagcctcaca ttatacgggg tactacaaaa aaaatgcagt accccggtgt agggaggttt
    14881 taacggcgtg ccagcaacag tcctagtacc agcccaacgg ccgcgcccac accaattcct
    14941 tgccagggtt tttcgtggac gtagtcatca gcacgataaa ctgcctgctt cgcccgatag
    15001 taataactgt ctgaagcctg gctaacccgt tttttcacat catccagcgc tttttctgca
    15061 cgcgctttca gctcaacata tttctgatcg gcgggatcgc cagaggagcg gagcacctct
    15121 tccagtgttt cactaagcag cgtcaggtcg tcatcgatac gtgtatcacc aaactgatta
    15181 gacattctcg ttctccattt gcgtaaaacc tgtcactaac tatagacaag ggttgttgat
    15241 tacgcctgaa ttacctcgcg tgccatccca atgtgtggga taccatcttc ttcatagacc
    15301 tccgtcacag agataaaacc gaaactctgg tagaagtttt gcagatgcgc ctgcgccccg
    15361 aggtaaacag gcttatcagg ccagtgatgc gtacatgttt cgagtgtttt actcatcagc
    15421 tgctgcccca ctttttcgcc acgcaacgct tcactgacaa tcacccgacc tataacgacc
    15481 ggctcaagat catcatcact tttcagaatc ctcgcatacg ccaccagttc atcatttttc
    15541 caaccgagaa tatggcgatt atcccccgtc aaatcatcac catcgatatc ctgataaggg
    15601 caattctgct cgacaacaaa caccgcgcaa cgcagttgta ataaggcata aagctgggaa
    15661 accgataatt cagagtgatg cagatcttgc cattcaatca taatgctctc cttttagatg
    15721 tatgccttat actagccgga atattttttg aaacgtgatg aactcatgga attaattttt
    15781 ttaggtactt cagccggtgt gccaacccgc acgcgcaatg tcacggcaat attgcttaac
    15841 ctgcaacatc cgacccagag cggactttgg ttgtttgact gcggtgaagg cacccagcat
    15901 cagctactgc ataccgcctt taaccctgga aaactggaca agatttttat cagtcacctt
    15961 catggcgatc atctttttgg tttacccggc ttgctgtgca gtcgttctat gtcaggcatt
    16021 atccaaccct taacgattta tggtccgcaa ggtatccgtg aatttgtgga aaccgcgctg
    16081 cggattagcg gctcatggac cgattatccg ctggaaattg tcgaaattgg cgctggcgaa
    16141 attctcgatg atggcctgcg caaagtaacc gcttatccgc tggaacaccc actggaatgt
    16201 tatggctatc gtattgaaga acatgataaa ccgggtgcat taaatgccca ggcattaaaa
    16261 gctgctggcg tgccgcctgg cccactgttt caggaattaa aagcgggcaa aacaatcacg
    16321 ctggaagatg gaaggcagat taacggcgca gattacttag ctgctccagt gccaggtaaa
    16381 gcgctcgcta ttttcggcga taccggcccc tgcgatgccg cacttgacct ggctaaaggt
    16441 gtcgatgtca tggtgcacga agcgacgctg gatataacca tggaagccaa agccaatagt
    16501 cgcggccata gctctacacg ccaggctgcg acactagccc gtgaggctgg agtcggtaag
    16561 ctaatcatta cccacgtcag ctcgcgctat gatgacaaag gttgtcagca cctgttacgt
    16621 gaatgcaggt caattttccc ggcgactgaa ctagcgaatg atttcaccgt gtttaacgtt
    16681 taactctgct tattattaaa aacagggcga aacttgccct gttatcgcaa cccgcgctac
    16741 atatatccac ccacacgggc atattgagcg atttttgata tttattcatc tccttacgaa
    16801 ctatattcag agcaataagt tgcctctttt tatcttcatt cagcacaacc tttgcaacta
    16861 aaaggagccc atgatggtta cagttgtcag caattattgt caattatctc aaacccaact
    16921 cagtcagaca tttgcagaaa aatttactgt gaccgaggaa ttactgcagt ctttaaaaaa
    16981 aacagcgtta tccggagatg aagaaagtat tgagttactg cataatattg cgttaggtta
    17041 tgatgaattt ggaaaaaaag ctgaagatat tctttaccat attgttagaa acccaacaaa
    17101 tgagacccta tcgattatcc gacttattaa aaatgcctgt ttaaaattat ataatttggc
    17161 acataccgca accaactccc accttaaacc aactggtccg gataattcag atgttctcct
    17221 gttcaaaaag ctattctccc cttcgaaatt aatgacaatt atcggtgatg aaattcctct
    17281 tatatctgaa aaacagtcgc tttcaaaggt gcttttaaat gatgagaata atgaactgag
    17341 tgatgataca aacttctggg ataaaaatcg tcaattaacc acagatgaaa tagcttgcta
    17401 tcttcagaag atcgccgcta atgcaaaaaa tactcaagtc aattatccta ctggtctcta
    17461 cgtcccctac tccaccagaa ctcacctgga agacgctctc aatgaaaata ttaagagcga
    17521 tccatcatga ccgaatgaag tccagttatt ccccataaat actggcg
//
LOCUS       DAPHDW010000093        17400 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.094, whole genome shotgun sequence.
ACCESSION   DAPHDW010000093 DAPHDW010000000
VERSION     DAPHDW010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.094"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(43..1326)
                     /gene="gltA"
                     /locus_tag="RH057_003061"
     CDS             complement(43..1326)
                     /gene="gltA"
                     /locus_tag="RH057_003061"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HDV3129396.1"
                     /translation="MADTKAKLTLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSDGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            complement(1532..1639)
                     /locus_tag="RH057_003062"
     CDS             complement(1532..1639)
                     /locus_tag="RH057_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129397.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            2020..2424
                     /gene="sdhC"
                     /locus_tag="RH057_003063"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             2020..2424
                     /gene="sdhC"
                     /locus_tag="RH057_003063"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HDV3129398.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILLWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            2418..2765
                     /gene="sdhD"
                     /locus_tag="RH057_003064"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             2418..2765
                     /gene="sdhD"
                     /locus_tag="RH057_003064"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HDV3129399.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            2765..4531
                     /gene="sdhA"
                     /locus_tag="RH057_003065"
     CDS             2765..4531
                     /gene="sdhA"
                     /locus_tag="RH057_003065"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HDV3129400.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALRDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            4547..5263
                     /gene="sdhB"
                     /locus_tag="RH057_003066"
     CDS             4547..5263
                     /gene="sdhB"
                     /locus_tag="RH057_003066"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HDV3129401.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            5464..8265
                     /gene="sucA"
                     /locus_tag="RH057_003067"
     CDS             5464..8265
                     /gene="sucA"
                     /locus_tag="RH057_003067"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HDV3129402.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELLEAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPSSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            8280..9497
                     /gene="odhB"
                     /locus_tag="RH057_003068"
     CDS             8280..9497
                     /gene="odhB"
                     /locus_tag="RH057_003068"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HDV3129403.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAADPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     EFLEDPTRLLLDV"
     gene            9591..10757
                     /gene="sucC"
                     /locus_tag="RH057_003069"
     CDS             9591..10757
                     /gene="sucC"
                     /locus_tag="RH057_003069"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HDV3129404.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            10757..11626
                     /gene="sucD"
                     /locus_tag="RH057_003070"
     CDS             10757..11626
                     /gene="sucD"
                     /locus_tag="RH057_003070"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HDV3129405.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGATASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(11730..12452)
                     /gene="mngR"
                     /locus_tag="RH057_003071"
     CDS             complement(11730..12452)
                     /gene="mngR"
                     /locus_tag="RH057_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HDV3129406.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITAQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            12621..14537
                     /gene="mngA"
                     /locus_tag="RH057_003072"
     CDS             12621..14537
                     /gene="mngA"
                     /locus_tag="RH057_003072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HDV3129407.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LEEVYRRESLGPTALGEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPPNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGDTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVRVAEPIRHAEALMQQALTLKRSDETRTVQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLMRWVKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLLTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            14555..17188
                     /gene="mngB"
                     /locus_tag="RH057_003073"
     CDS             14555..17188
                     /gene="mngB"
                     /locus_tag="RH057_003073"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HDV3129408.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRVKKQVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITRTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVLPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEQIEAQRESLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKIAHARIENKIVNLLEPLATLAWTLGFEYHHGLLEKMWKEILK
                     NHAHDSIGCCCSDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVMTSATAKPQCEITHEAWQSRAVIR
                     YDMAVPLNLLERSVRQSTGRVGVEMVVTLSHNSRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQEGWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLFLRPGRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
BASE COUNT         4205 a         4332 c         4737 g         4126 t
ORIGIN      
        1 ttgaacggcg ggttaaaata tttacaactt agcaatcaac cattaacgct tgatatcgct
       61 tttaaagtcg cgtttttcat atcctgtata cagctgacgc ggacgggcaa tcttcatacc
      121 gtcactgtgc atttcgctcc agtgggcgat ccagccaacg gtacgtgcca ttgcgaaaat
      181 gacggtgaac atggaagacg gaatacccat cgctttcagg atgataccag agtagaaatc
      241 gacgttcggg tacagtttct tctcgataaa gtacgggtcg ttcagcgcga tgttttccag
      301 ctccatagcc acttccagca ggtcatcttt ggtgcccagc tctttcagaa cttcatggca
      361 ggtttcacgc attacggtgg cgcgcgggtc gtaatttttg tacacgcggt gaccgaagcc
      421 catcaggcgg aaagaatcat ttttatcttt cgcacgacga acaaattccg gaatgtgttt
      481 aacggagcta atttcttcca gcattttcag cgccgcttcg ttagcaccac cgtgcgcagg
      541 tccccacagt gaagcaatac ctgctgcgat acaggcaaac gggttcgcac ccgaagagcc
      601 agcggtacgc acggtggagg tagaggcgtt ctgttcatgg tcagcgtgca ggatcagaat
      661 acggtccata gcacgttcca gaatcggatt aacttcatac ggttcgcacg gcgtggagaa
      721 catcatattc aggaagttac cggcatagga gagatcgttg cgcggataaa caaatggctg
      781 accaatggaa tacttgtaac acatcgcggc catggtcggc attttcgaca gcaggcggaa
      841 cgcggcaatt tcacgatgac gaggattgtt aacatccagc gagtcgtgat agaacgccgc
      901 cagcgcgccg gtaataccac acatgactgc cattggatgt gagtcgcgac ggaaagcgtg
      961 gaacagacgg gtaatctgct cgtggatcat ggtatgacgg gtcaccgtag ttttaaattc
     1021 gtcatactgt tcctgagtcg gtttttcacc attcagcagg atgtaacaaa cttccaggta
     1081 gttagaatcg gtcgccagct gatcgatcgg gaaaccgcgg tgcagcaaaa taccttcatc
     1141 accatcaata aaagtaattt tagattcgca ggatgcggtt gaagtgaagc ctgggtcaaa
     1201 ggtgaacaca ccttttgaac cgagagtacg gatatcaata acatcttgac ccagcgtgcc
     1261 tttcagcaca tccagttcaa cagctgtgtc cccgttgagg gtgagttttg cttttgtatc
     1321 agccatttaa ggtctcctta gcgccttatt acgtaagact gccggaactt aaatttgcct
     1381 tcgcacatca acctggcttt acccgtttgt tatttggctc gccgctctgt gaaagagggg
     1441 aaaacctggg tacagagctc tgggcgcttg caggtaaagg atccattgat gacgaataaa
     1501 tggcgaatca agtacttagc aatccgaatt attaaacttg tctaccacta ataactgtcc
     1561 cgaatgaatt ggtcaatact tccacactgt tacataagtt aatcttaggt gaaataccga
     1621 cttcataact tttacgcatt atatgctttt cctggtaatg tttgtaacaa ctttgttgaa
     1681 tgattgtcaa attagatgat taaaaattaa ataaatgttg ttatcgtgac ctggatcact
     1741 gttcaggata aaacccgaca aactatatgt aggttaattg taatgatttt gtgaacagcc
     1801 tatactgccg ccaggtctcc ggaacaccct gcaatcccga gccacccagc gttgtaacgt
     1861 gtcgttttcg catctggaag cagtgttttg catgacgcgc agttatagaa aggacgctgt
     1921 ctgacccgca agcagaccgg aggaaggaaa tcccgacgtc tccaggtaac agaaagttaa
     1981 cctctgtgcc cgtagtcccc agggaataat aagaacagca tgtgggcgtt attcatgata
     2041 agaaatgtga aaaaacaaag acctgttaat ctggacctac agaccatccg gttccccatc
     2101 acggcgatag cgtccattct ccatcgcgtt tccggtgtga tcacctttgt tgcagtgggc
     2161 atcctgctgt ggcttctggg taccagcctc tcttcccctg aaggtttcga gcaagcttcc
     2221 gcgattatgg gcagcttctt cgtcaaattt atcatgtggg gcatccttac cgctctggcg
     2281 tatcacgtcg tcgtaggtat tcgccacatg atgatggatt ttggctatct ggaagaaaca
     2341 ttcgaagcgg gtaaacgctc cgccaaaatc tcctttgtta ttactgtcgt gctttcactt
     2401 ctcgcaggag tcctcgtatg gtaagcaacg cctccgcatt aggacgcaat ggcgtacatg
     2461 atttcatcct cgttcgcgct accgctatcg tcctgacgct ctacatcatt tatatggtcg
     2521 gttttttcgc caccagtggc gaactgacat atgaagtctg gatcggtttc ttcgcctctg
     2581 cgttcaccaa agtgttcacc ctgctggcgc tgttttctat cttgattcat gcctggatcg
     2641 gcatgtggca ggtgttgacc gactacgtta aaccgctggc attgcgcctg atgctgcaac
     2701 tggtgattgt cgttgcactg gtggtttacg tgatttatgg attcgttgtg gtgtggggtg
     2761 tgtgatgaaa ttgccagtca gagaatttga tgcagttgtg attggtgccg gtggcgcagg
     2821 tatgcgcgcg gcgctgcaaa tttcccagag cggccagacc tgtgcgctgc tctctaaagt
     2881 cttcccgacc cgttcccata ccgtttctgc gcaaggcggc attaccgttg cgctgggtaa
     2941 tacccatgaa gataactggg aatggcatat gtacgacacc gtaaaagggt cggactatat
     3001 cggtgaccag gacgcgattg aatatatgtg taaaaccggg ccggaagcga ttctggaact
     3061 cgaacacatg ggcctgccgt tctcgcgtct tgatgatggt cgtatctatc aacgtccgtt
     3121 tggcggtcag tcgaaaaact tcggcggcga gcaggcggca cgcactgcgg cggcggctga
     3181 ccgtaccggt cacgcactgt tgcacacgct ttatcagcag aacctgaaaa accacaccac
     3241 cattttctcc gagtggtatg cgctggatct ggtgaaaaac caggatggcg cggtggtggg
     3301 ttgtaccgca ctgtgcatcg aaaccggtga agtggtttat ttcaaagccc gcgctaccgt
     3361 gctggcgact ggcggggcag ggcgtattta tcagtccacc actaacgccc acattaacac
     3421 tggcgacggc gtcggcatgg ctatccgcgc aggcgtaccg gtgcaggata tggaaatgtg
     3481 gcagttccac ccgaccggta ttgccggtgc gggcgtactg gtcaccgaag gttgccgtgg
     3541 tgaaggcggt tatctgctga acaaacatgg cgaacgcttt atggagcgtt atgcgccgaa
     3601 cgccaaagac ctggcgggcc gtgacgtggt ggcgcgttcc atcatgattg aaatccgtga
     3661 aggtcgcggc tgtgatggtc cgtgggggcc acatgcgaaa ctgaaactcg accacctggg
     3721 taaagaagtt ctcgaatccc gtctgccggg tattctggaa ctctcccgta ccttcgctca
     3781 cgttgatccg gtgaaagagc cgattccggt tatcccaacc tgtcactaca tgatgggtgg
     3841 tattccgacc aaagtgaccg gtcaggcgct gacggtgaac gagaaaggcg aagatgtggt
     3901 tgttccgggg ctgtttgccg ttggtgaaat cgcttgtgta tcggtacacg gcgctaaccg
     3961 tctgggcggc aactcgctgc tggacctggt ggtctttggt cgcgcagcgg gtctgcatct
     4021 gcaagagtct atcgccgagc agggcgcatt gcgcgatgcc agcgagtctg atgttgaagc
     4081 atctctggat cgtctgaacc gctggaacaa taatcgtaac ggtgaagatc cggtggcgat
     4141 tcgtaaagcg ctgcaagaat gtatgcagca taacttctcg gtcttccgtg aaggtgatgc
     4201 gatggcgaaa gggcttgagc agttgaaagt tatccgcgag cgtctgaaaa atgcccgtct
     4261 ggatgacact tccagcgagt tcaacaccca gcgcgttgag tgcctggaac tggataacct
     4321 gatggaaacg gcgtatgcaa cggctgtttc tgccaacttc cgtaccgaaa gccgtggcgc
     4381 gcatagccgc ttcgacttcc cggatcgtga tgatgaaaac tggctgtgcc actccctgta
     4441 tctgccagag tcggaatcca tgacgcgccg aagcgtcaac atggaaccga aactgcgccc
     4501 ggcattcccg ccgaagattc gtacttacta atgcggagac aggaaaatga gactcgagtt
     4561 ttcaatttat cgctataacc cggatgttga tgatgctccg cgtatgcagg attacaccct
     4621 ggaagcggaa gaaggtcgcg acatgatgtt gctggatgcg cttatccagc tgaaagagaa
     4681 agatcccagc ctgtcgttcc gccgctcctg ccgtgaaggt gtgtgcggtt ccgacggtct
     4741 gaacatgaac ggcaagaatg gtctggcctg tattaccccg atttcggcac tcaaccagcc
     4801 gggcaagaag attgtgattc gcccgctgcc aggtttaccg gtgatccgcg atttggtggt
     4861 agacatggga caattctatg cgcaatatga gaaaattaag ccttacctgt tgaataatgg
     4921 acaaaatccg ccagctcgcg agcatttaca gatgccagag cagcgcgaaa aactcgacgg
     4981 gctgtatgaa tgtattctct gcgcatgttg ttcaacctct tgtccgtctt tctggtggaa
     5041 tcccgataag tttatcggcc cggcaggctt gttagcggca tatcgtttcc tgatcgatag
     5101 ccgtgatacc gagactgaca gccgcctcga cggtttgagc gatgcattca gtgtattccg
     5161 ctgtcacagc atcatgaact gcgtcagtgt atgtccgaag gggctgaacc cgacgcgcgc
     5221 catcggccat atcaagtcga tgttgttgca acgtaatgcg taattaaatg ttgatgccgg
     5281 ggaaatcccc cggctattca ggaaatcttt aaaaactgcc cctgacacta agacagtttt
     5341 taaaggttcc ttcgcgagcc actacgtaga caagagctcg caagtgaacc ccggcacgca
     5401 cataactgtg cgtggtagta tccacggcga agtaagcata aaaaagatgc ttaagggatc
     5461 acgatgcaga acagcgcttt gaaagcctgg ttggactctt cttacctctc tggcgcaaac
     5521 cagagctgga tagaacagct ctatgaagac ttcttaaccg atcctgactc ggttgacgct
     5581 aactggcgtt cgacgttcca gcagttacct ggtacgggag tcaaaccgga tcaattccac
     5641 tctcaaacgc gtgaatattt ccgccgcctg gcgaaagacg cttcacgtta ctcttcaacg
     5701 atctccgacc ctgacaccaa tgtaaagcag gttaaagtcc tgcagctcat taacgcatac
     5761 cgcttccgtg gtcaccagca tgcgaatctc gatccgctgg gactgtggca gcaagataaa
     5821 gtggccgatc tggatccgtc tttccacgat ctgaccgaag cagacttcca ggagaccttc
     5881 aacgtcggtt catttgccag cggcaaagaa accatgaaac tcggcgagct gctggaagcc
     5941 ctcaagcaaa cctactgcgg cccgattggt gccgagtata tgcacattac cagcaccgaa
     6001 gaaaaacgct ggatccaaca gcgtatcgag tctggtcgcg cgactttcaa tagcgaagag
     6061 aaaaaacgct tcttaagcga actgaccgcc gctgaaggcc ttgaacgtta cctcggcgca
     6121 aaattccctg gcgcaaaacg cttctcgctg gaaggcggtg acgcgttaat cccgatgctt
     6181 aaagagatga tccgccacgc tggcaacagc ggcacccggg aagtggttct cgggatggcg
     6241 caccgtggtc gtctgaacgt gttggtgaac gtgctgggta aaaaaccgca agacttgttc
     6301 gacgagtttg ccggtaaaca taaagaacac ctcggcacgg gtgacgtgaa ataccacatg
     6361 ggcttctcgt ctgacttcca gaccgatggc ggcctggtgc acctggcgct ggcgtttaac
     6421 ccgtctcacc ttgagattgt aagcccggtc gttatcggct ctgttcgtgc ccgtctggac
     6481 agacttgatg agccgagcag caacaaagtg ctgccaatta ccatccacgg tgacgccgca
     6541 gtgaccggac agggcgtggt tcaggaaacc ctgaacatgt cgaaagcgcg tggttatgaa
     6601 gttggcggta cggtacgtat cgttatcaac aaccaggttg gtttcaccac ctctaatccg
     6661 ctggatgccc gttctacgcc gtactgtact gatatcggca agatggttca ggcaccgatt
     6721 ttccacgtta acgcggacga tccggaagcc gttgcctttg tgacccgtct ggcgctcgat
     6781 ttccgtaaca cctttaaacg tgatgtcttc atcgacctgg tatgctaccg ccgtcacggc
     6841 cacaacgaag ccgacgagcc gagcgcaacc cagccgctga tgtatcagaa aatcaaaaaa
     6901 catccgacac cgcgcaaaat ctacgctgac aagctggagc aggaaaaagt ggcgacgctg
     6961 gaagatgcca ccgagatggt taacctgtac cgcgatgcgc tggatgctgg cgattgcgtg
     7021 gtagcagagt ggcgtccgat gaacatgcac tctttcacct ggtcgccgta cctcaaccac
     7081 gaatgggacg aagagtaccc gaacaaagtt gagatgaagc gcctgcagga actggctaaa
     7141 cgcatcagca cggtgccgga agcggttgaa atgcagtctc gcgttgccaa gatttatggc
     7201 gatcgccagg cgatggctgc cggtgagaaa ctgttcgact ggggcggcgc ggaaaacctc
     7261 gcttacgcca cgctggttga cgaaggcatt ccggttcgcc tgtcgggtga agactccggt
     7321 cgcggtacct tcttccaccg ccacgcggtg atccacaacc agtctaacgg ttccacttac
     7381 acgccgctgc aacacatcca taacggccag ggcgcgttcc gtgtttggga ctccgtactg
     7441 tctgaagaag cagtgctggc gtttgaatac ggttatgcca ccgcagaacc acgcaccctg
     7501 accatctggg aagcgcagtt cggtgacttc gccaacggtg cgcaggtggt tatcgaccag
     7561 ttcatctcct ctggcgagca gaaatggggc cggatgtgtg gtctggtgat gttgctgccg
     7621 cacggttacg aagggcaggg gccggagcac tcctccgcgc gtctggaacg ttatctgcaa
     7681 ctttgtgctg agcaaaacat gcaggtttgc gtaccgtcta ccccggcaca ggtttaccac
     7741 atgctgcgtc gtcaggcgct gcgcgggatg cgtcgtccgc tggtcgtgat gtcgccgaaa
     7801 tccctgctgc gtcatccgct ggcggtttcc agcctcgaag aactggcgaa cggcaccttc
     7861 ctgccagcca tcggtgaaat cgacgagctt gatccgaagg gcgtgaagcg cgtagtaatg
     7921 tgttctggta aggtttatta cgacctgctg gaacagcgtc gtaagaacaa tcaacacgat
     7981 gtcgccattg tgcgtatcga gcaactctac ccgttcccgc ataaagcgat gcaggaagtg
     8041 ttgcagcagt ttgctcacgt caaggatttt gtctggtgcc aggaagagcc gctcaaccag
     8101 ggcgcatggt actgcagcca gcatcatttc cgtgaagtga ttccgtttgg ggcttctctg
     8161 cgttatgcag gccgcccggc ctccgcctct ccggcggtag ggtatatgtc cgttcaccag
     8221 aaacagcaac aagatctggt taatgacgcg ctgaacgtcg aataaataaa ggatacataa
     8281 tgagtagcgt agatattctg gtccctgacc tgcctgaatc cgtagccgat gccaccgtcg
     8341 caacctggca taaaaaaccc ggcgacgcag tcgtacgtga tgaagtgctg gtagaaatcg
     8401 aaactgacaa agtggtactg gaagtaccgg catcagcaga cggcattctg gatgcggttc
     8461 tggaagatga aggtacaacg gtaacgtctc gtcagatcct tggtcgcctg cgtgaaggca
     8521 acagcgccgg taaagaaacc agcgccaaat ctgaagagaa agcgtccact ccggcgcaac
     8581 gccagcaggc gtctctggaa gagcaaaaca acgatgcgtt aagcccggcg atccgtcgcc
     8641 tgctggctga acacaatctc gacgccagcg ccattaaagg caccggtgtg ggtggtcgtc
     8701 tgactcgtga agatgtggaa aaacatctgg cgaaagcccc ggcgaaagag tctgctccgg
     8761 cagcggctga tccggcggcg caaccggctc tggctgcacg tagtgaaaaa cgtgtcccga
     8821 tgactcgcct gcgtaagcgt gtggcagagc gtctgctgga agcgaaaaac tccaccgcca
     8881 tgctgaccac gttcaacgaa gtcaacatga agccgattat ggatctgcgt aagcagtacg
     8941 gtgaagcgtt tgaaaaacgc cacggcatcc gtctgggctt tatgtccttc tacgtgaaag
     9001 cggtggttga agccctgaaa cgttacccgg aagtgaacgc gtctatcgac ggcgatgacg
     9061 tggtttacca caactatttc gacgtcagca tggcggtttc tacgccgcgc ggcctggtga
     9121 caccggtact gcgtgatgtc gataccctcg gcatggcaga catcgagaag aaaatcaaag
     9181 agctggcagt caaaggccgt gacggcaagc tgaccgttga agatctgacc ggtggtaact
     9241 tcaccatcac caacggtggt gtgttcggtt ccctgatgtc tacgccgatc atcaacccgc
     9301 cgcagagcgc aattctgggt atgcacgcta tcaaagatcg tccgatggcg gtgaatggtc
     9361 aggttgagat cctgccgatg atgtacctgg cgctgtccta cgatcaccgt ctgatcgatg
     9421 gtcgcgaatc cgtgggcttc ctggtaacga tcaaagagtt tctggaagat ccgacgcgtc
     9481 tgctgctgga cgtgtagtag tttaagcttc acctgcactg tagaccgacg tggatagggt
     9541 gtcggtctac ggtttaaaag ataacgatta ctgaaggatg gacagaacac atgaacttac
     9601 atgaatatca ggcaaaacaa ctttttgccc gctatggctt accagcaccg gtgggttatg
     9661 cctgtactac tccgcgcgaa gcagaagaag ccgcttcaaa aatcggtgcc ggcccgtggg
     9721 tagtgaaatg tcaggttcac gctggtggcc gcggtaaagc gggcggtgtg aaagttgtaa
     9781 acagcaaaga agacatccgt gcttttgcag aaaactggct cggcaagcgt ctggtaacgt
     9841 atcaaacaga tgccaatggc caaccggtta accagattct ggttgaagca gcgaccgata
     9901 tcgctaaaga gctgtatctc ggtgccgttg tagaccgtag ttcccgtcgc gtggtcttta
     9961 tggcctccac cgaaggcggc gtggaaatcg aaaaagtggc ggaagaaact ccgcacctga
    10021 tccataaagt tgcgcttgat ccgctgacag gcccgatgcc gtatcaggga cgcgagctgg
    10081 cgttcaaact gggtctggaa ggtaaactgg ttcagcagtt caccaaaatc ttcatgggcc
    10141 tggcgaccat tttcctggag cgcgacctgg cgttgatcga aatcaacccg ctggtcatca
    10201 ccaaacaggg cgatctgatt tgcctcgacg gcaaactggg cgctgacggc aacgcactgt
    10261 tccgccagcc tgatctgcgc gaaatgcgtg accagtcgca ggaagatccg cgtgaagcac
    10321 aggctgcaca gtgggaactg aactacgttg cgctggacgg taacatcggt tgtatggtta
    10381 acggcgcagg tctggcgatg ggtacgatgg acatcgttaa actgcacggc ggcgaaccgg
    10441 ctaacttcct tgacgttggc ggcggcgcaa ccaaagaacg tgtaaccgaa gcgttcaaaa
    10501 tcatcctctc tgacgacaaa gtgaaagccg ttctggttaa catcttcggc ggtatcgttc
    10561 gttgcgacct gatcgctgac ggtatcatcg gcgcggtagc agaagtgggt gttaacgtac
    10621 cggtcgtggt acgtctggaa ggtaacaacg ccgaactcgg cgcgaagaaa ctggctgaca
    10681 gcggcctgaa tattattgca gcaaaaggtc tgacggatgc agctcagcag gttgttgccg
    10741 cagtggaggg gaaataatgt ccattttaat tgataaaaac accaaggtta tctgccaggg
    10801 ctttactggt agccagggga ctttccactc agaacaggcc attgcatacg gcactaaaat
    10861 ggttggcggc gtaaccccag gtaaaggcgg tactacccac ctcggcctgc cggtgttcaa
    10921 caccgtgcgt gaagctgttg ctgctactgg cgctaccgct tctgttatct acgtaccagc
    10981 accgttctgc aaagactcca ttctggaagc tattgatgca ggcatcaaac tgattatcac
    11041 catcactgaa ggcatcccga cgctggatat gctgactgtg aaagtgaaac tggacgaagc
    11101 cggcgttcgt atgatcggcc cgaactgccc gggcgttatc actccgggtg aatgcaaaat
    11161 cggtatccag ccgggtcaca ttcacaaacc gggtaaagtg ggtatcgttt cccgttccgg
    11221 tacactgacc tatgaagcgg ttaaacagac cacggattac ggtttcggtc agtcgacctg
    11281 tgttggtatc ggcggtgacc cgatccctgg ctctaacttt atcgacattc tcgaaatgtt
    11341 cgaaaaagat ccgcagaccg aagcgatcgt aatgatcggt gagatcggcg gtagcgctga
    11401 agaagaagcg gcagcgtaca tcaaagagca tgttaccaag ccagttgtgg gttacatcgc
    11461 tggtgtgact gcgccgaaag gcaaacgtat gggccacgcg ggtgccatca ttgccggtgg
    11521 gaaagggact gcggatgaga aatttgctgc tctggaagcc gcaggcgtga aaaccgttcg
    11581 cagcctggcg gatatcggtg aagcactgaa aactgttctg aaataaatat ctgtaataag
    11641 aaatagccct cgccgcttcc ctctacagga atggcgaagg gctgtcggtt tcgacatggt
    11701 tggccatcgt atgatggcct tttttgtgct tatcgcgatg attttcgttg cgctatcagg
    11761 gtaaatttat agtcatcggt attaaaagcg ttgcggctat attcaaacac ccgaccatca
    11821 actaaatatc cacgcgatac tttttcaaga atcggctttg tctggctgat attaagcaga
    11881 cggctcatct cttcggttgg catcagagga atgatttcct gttcgctacg atcgataacc
    11941 attttcttca cttcttcaat aaagtgatat tttgaatttt ccatgacctg ccaggtgaga
    12001 tccgggaaca acgcaagcgg catccaggtt tcttccagcg ccattggctt ttgcttgcga
    12061 tagcgcacgc gcttcacatg ccacacacga tcctgcgcgg tgatttgtag ctgttgctga
    12121 agaaaatcgt cagccggaat cacttcaaat atcaggactt cactgtgtgt atcgacgtga
    12181 cggtccgaca gtttttcatc aaaactggtt aactgaaaaa tatcgtaatt gacccgctct
    12241 tctttgacgt aagtcccgct gccctgaatg ctttcgagga tctgttgctc gactaactgg
    12301 cgcaaagcct gacgcaccgt aacccggctg acgccaaact ctgtttgtaa tgccgattcg
    12361 gtaggtaacg catcgccagg tttaagctcg ccacgcgcaa tttgttcacg aatgcgatcg
    12421 gcaatctgcc ggtataaggg cttgtgtccc atttttagta tctcattaat acgaatttag
    12481 ccattatgcc cgataaattc atcctgtaaa taatacaaat acaatacaaa taatttcaat
    12541 caagtgaaat tgatcacata atggtattgt tttatcgggc acactggcgc gactataaaa
    12601 acgatcaagt gaggatcatg atgaatctga cgactctgac ccaccgcgat gcgttgtgtc
    12661 tgaatgcgcg ctttaccagc cgtgaagagg ccatccacgc gttgactcaa cgtcttgctg
    12721 ctctggggaa aatttccagt actgagcaat ttctggaaga agtgtatcgc cgtgaaagcc
    12781 ttggcccgac cgccttaggt gaagggttgg ctgtgccgca tggcaaaact gctgcggtaa
    12841 aagaagcggc gtttgcggtc gccacactca gcgagccgct tcagtgggaa ggcgttgatg
    12901 gcccggaagc ggttgattta gtggtgctgc tggcgattcc ccccaatgaa gcgggtacta
    12961 cgcatatgca actgctgaca gcgctgacca cgcgcctggc ggatgatgag attcgggcgc
    13021 gtatacagtc ggcgacgacg cctgatgagt tgctctcggc gctggatgac aagggagaca
    13081 cgcaaccttc tgcctctttt tccaacgcgc caactatcgt ctgcgtaacg gcctgtccgg
    13141 cgggtattgc tcacacctat atggctgcgg aatatctgga aaaagccgga cgcaaactcg
    13201 gcgtaaatgt ttacgttgaa aaacaaggcg ctaacggcat tgaagggcgt ttaacggcgg
    13261 atcaactcaa tagtgcaacc gcctgtattt ttgcggctga agtcgccatc aaggagagtg
    13321 agcgttttaa tggcattccc gcgctttcag tgcgtgttgc cgagccgatt cgccatgcag
    13381 aagcgttgat gcaacaagcg cttaccctca agcgtagcga tgagacgcgt accgtacagc
    13441 aagatacgca accggtgaaa agtgtcaaaa cggagctgaa acaggcactg ttgagcggaa
    13501 tctcttttgc cgtaccgttg attgtcgcgg ggggcacggt gctggcggtc gcggtattac
    13561 tgtcgcaaat cttcgggcta caagatctgt ttaatgaaga aaactcctgg ctgtggatgt
    13621 accgcaagct gggcggcggg ctgctcggaa ttttgatggt accggtgctc gcggcctata
    13681 ccgcctattc tctggcagat aaaccggcgt tagcgccagg ctttgcggct ggacttgccg
    13741 ccaacatgat cggctccggg tttctcggcg cggtcgttgg cggattgata gccggttact
    13801 tgatgcgctg ggtgaaaaat cacttgcgtc ttagcagtaa attcaatgga ttcctgactt
    13861 tttatctcta cccggtgctc ggtacgttgg gagcgggcag tctgatgctg tttgtggtgg
    13921 gagaacctgt cgcctggatc aataactcgc ttaccgcctg gctgaacggt ctgtcaggaa
    13981 gtaacgcgct gttgctgggt gccattctcg gttttatgtg ttcctttgac cttggagggc
    14041 cagtgaacaa agccgcttat gcattctgcc tgggcgcaat ggcgaacggc gtttacggcc
    14101 cgtatgccat tttcgcctcc gtcaaaatgg tttcggcatt taccgtaacc gcttccacga
    14161 tgctcgcacc gcgcctgttt aaagagtttg aaattgagac cgggaaatcc acctggctgt
    14221 tagggctggc aggtattacc gaaggggcga tcccgatggc gattgaagat ccgctgcggg
    14281 ttattggttc gtttgtgctg ggctctatgg taacgggcgc tattgtcggt gcgatgaata
    14341 tcggcctttt gacacccggt gccggcattt tctcgctctt tttacttcat gataatggcg
    14401 cgggcggtgt tatggcggca attggctggt ttggtgcggc attggtgggg gctgcaatct
    14461 cgactgcaat tctcctgatc tggcggcgtc acgcggttaa gcatggcaac tatctgactg
    14521 atggcgtaat gccataaaca aaaaggaaac gacgatgaaa gcggtatctc gcgttcacat
    14581 caccccgcat atgcattggg atcgggagtg gtatttcacc accgaagagt cacgcattct
    14641 gctggtcaat aatatggaag agatcctgtg ccgactggaa caggacaacg aatacaaata
    14701 ttacgtactc gacgggcaaa cggcgatcct cgaagattat ttcgcggtga aaccggaaaa
    14761 caaagaccgc gtgaagaaac aggtagaagc cggtaagttg attatcggcc cctggtatac
    14821 ccagaccgat accacgattg tttctgcgga atccatcgtc cgtaatctga tgtacggaat
    14881 gcgtgactgc ctcgcgtttg gcgagccgat gaaaataggt tatttaccgg attcctttgg
    14941 catgtccggg caactgccgc atatctacaa tggatttggc attacccgca ctatgttctg
    15001 gcgcggatgt tcggagcgcc acggtactga taaaaccgag tttttatggc aaagcagtga
    15061 cggtagcgaa gtgacggcgc aggtgctgcc gttgggttat gccatcggta agtacttacc
    15121 tgccgacgaa aacggattac gcaaacgcct cgacagttat tttgacgtgc tggaaaaagc
    15181 gtctgtaacc aaagagattt tattgccgaa tggtcatgac cagatgccat tgcagcaaaa
    15241 tatcttcgaa gtgatggaga aacttcgcga aatttatcct caacgaaagt ttgtgatgag
    15301 ccgctttgaa gaggtattcg agcagatcga agcgcagcgt gaaagtctgg ccaccctgaa
    15361 aggtgaattt atcgatggca aatatatgcg cgtacatcgc accatcggtt ccacgcgcat
    15421 ggatatcaaa attgcccacg cacgtattga aaataagatt gttaatctgc tggaaccgct
    15481 ggcaacgctg gcctggacgt tgggttttga ataccaccac ggcttgctgg agaaaatgtg
    15541 gaaagagatc ttaaaaaatc atgcccacga cagtatcggt tgttgttgca gtgacaaagt
    15601 tcatcgcgaa attgtcgccc gcttcgaact ggctgaagac atggcggata atctgctgcg
    15661 tttctacatg cgcaaaattg ccgataacat gccacagagt gatgccgaca aactcgtgct
    15721 gtttaacctg atgccctggc cgcgggaaga agttattaac accactgtgc ggctgcgcgc
    15781 cagccagttt aatctacgag acgatcgcgg tcagcctgta ccgtatttta ttcgccatgc
    15841 ccgtgagatc gatccaggcc taatcgatcg gcaaatagtt cattacggta attacgatcc
    15901 ctttatggag tttgatatac agatcaacca gattgtccct tctatgggct atcgcacgct
    15961 ttatatcgaa gcgaatcagc ctggcaacgt gattgcggca aaaagtgacg ctgaagggat
    16021 actggaaaat gctttctggc aaattgcgct caatgaggat ggaacgctac aactggtaga
    16081 taaagatagc ggtgtgcgct atgaccgggt gttgcaaatt gaagaaagct ctgatgatgg
    16141 tgatgaatat gactattcac ccgcaaaaga agagtgggta atgacttctg caacagcgaa
    16201 accgcaatgc gagattactc atgaggcctg gcagagcagg gctgttatcc gctatgacat
    16261 ggcggtgccg ctcaatttgt tggaacgaag cgtccggcaa tccacgggca gagtaggggt
    16321 ggagatggtt gtcactctga gtcataacag cagacgtatt gatgtggata tcaaccttga
    16381 taaccaggct gacgatcatc gccttcgcgt cctgatccct acatctttta acaccgatag
    16441 tgttctggca gatacccagt ttggttcgct aacgcgcccc gtgaacgaca gtgcaatgaa
    16501 caactggcag caagaaggct ggaaagaagc gccggttccg gtatggaata tgctcaacta
    16561 tgcagcctta caggaagggc gtaacggcat ggctgtcttt agcgaagggc tacgtgaatt
    16621 tgaagtcatc ggtgaagaga agaaaacctt tgccattacg ttgctgcgtg gcgtgggctt
    16681 actgggcaaa gaagatctgt ttttaaggcc tgggcggcct tcgggaatta aaatgccagt
    16741 cccggactca caactacgtg gtctgctttc ttgtcgccta agtttattga gttataccgg
    16801 tacgccaacc gccgctggtg tagctcagca ggcgcgagca tggctgactc cagtacagtg
    16861 ttacaacaaa atcccatggg atgcgatgaa gctcaacaaa gccggattca acgtgccgga
    16921 aagttatagt ttgttgaaaa tgcccccagt gggatgcctg ataagcgcac ttaagaaagc
    16981 tgaagaccga caagaagtga ttttacggct gtttaatccg gctgaatcag caacctgtga
    17041 tgcgactgtt gctttcagtc gcgaggtgat ttcttgctca gaaacgatga tggatgaaca
    17101 cattaccacc gaggaaaatc aaggttcaaa tctatcgggg ccttttttac ccggccagtc
    17161 acggacgttc agttaccggc ttgcctgaat agcaatcaaa ccgaagccac atatgcgcgg
    17221 ccagattgtt gacaaagggc gctttgttca tgccggatac ggcatgaacg ctttattcgg
    17281 tctacaaaag caggcaaatt caatatattg cagagatgat gtagacactg gcaagcgtag
    17341 cgcatcaggt aattttgcgt ttatcttcac tctcaagcca cgtatatgtg gctttatttt
//
LOCUS       DAPHDW010000094        16774 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.095, whole genome shotgun sequence.
ACCESSION   DAPHDW010000094 DAPHDW010000000
VERSION     DAPHDW010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16774)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16774)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16774
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.095"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1037
                     /gene="ompN"
                     /locus_tag="RH057_003074"
     CDS             <1..1037
                     /gene="ompN"
                     /locus_tag="RH057_003074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="porin OmpN"
                     /protein_id="HDV3129409.1"
                     /translation="LYGKVDGLHYFSDNSAKDGDQSYARLGFKGETQINDQLTGYGQW
                     EYNIQANNTESSKNQSWTRLAFAGLKFADYGSFDYGRNYGVMYDIEGWTDMLPEFGGD
                     SYTNADNFMTGRANGVATYRNTDFFGLVNGLNFAVQYQGNNEGASNGQEGTNNGRDVR
                     HENGDGWGLSTTYDLGMGFSAGAAYTSSDRTNDQVNHTAAGGDKADAWTAGLKYDANN
                     IYLATMYSETRNMTPFGDSDYAVANKTQNFEVTAQYQFDFGLRPAVSFLMSKGRDLHA
                     AGGADNPAGVDDKDLVKYADVGATYYFNKNMSTYVDYKINLLDEDDSFYAANGISTDD
                     IVALGLVYQF"
     gene            1178..1612
                     /gene="uspF"
                     /locus_tag="RH057_003075"
     CDS             1178..1612
                     /gene="uspF"
                     /locus_tag="RH057_003075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HDV3129410.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEM
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            1788..2723
                     /gene="ttcA"
                     /locus_tag="RH057_003076"
     CDS             1788..2723
                     /gene="ttcA"
                     /locus_tag="RH057_003076"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HDV3129411.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGATKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(2852..4225)
                     /gene="dbpA"
                     /locus_tag="RH057_003077"
     CDS             complement(2852..4225)
                     /gene="dbpA"
                     /locus_tag="RH057_003077"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HDV3129412.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVGQSALSLHGDLE
                     QRDRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTAR
                     AGNSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVPLEAEMATLCIDGGKK
                     AKMRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKT
                     CRVRLLK"
     gene            complement(4703..5686)
                     /gene="zntB"
                     /locus_tag="RH057_003078"
     CDS             complement(4703..5686)
                     /gene="zntB"
                     /locus_tag="RH057_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HDV3129413.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRLGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            5941..7173
                     /gene="dgcM"
                     /locus_tag="RH057_003079"
     CDS             5941..7173
                     /gene="dgcM"
                     /locus_tag="RH057_003079"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
                     /protein_id="HDV3129414.1"
                     /translation="MITHNFNTLDLLTSPVWIVSPFEEQLIYANSAARLLMQDLTFSQ
                     LRTGPYSVSSQKELPKYLSDLQNQHDIIEILTVQRKEEETALSCRLVLRELTETEPVI
                     IFEGIEAPATLGLKASRSANYQRKKQGFYARFFLTNSAPMLLIDPSRDGQIVDANLAA
                     LNFYGYNHETMCQKHTWEINMLGRRVMPIMHEISHLPGGHKPLNFLHKLADGSTRHVQ
                     TYAGPIEIYGDKLMLCIVHDITEQKRLEEQLEHAAHHDAMTGLLNRRQFYHITEPGQM
                     QHLAIAQDYSLLLIDTDRFKHINDLYGHSKGDEVLCALARTLESCARKGDLVFRWGGE
                     EFVLLLPRTPLDTALSLAETIRVSVAKVSISGLPRFTVSIGVAHHEGNESIDELFKRV
                     DDALYRAKNDGRNRVLAA"
     gene            complement(7194..7757)
                     /gene="smrA"
                     /locus_tag="RH057_003080"
     CDS             complement(7194..7757)
                     /gene="smrA"
                     /locus_tag="RH057_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
                     /protein_id="HDV3129415.1"
                     /translation="MNLDDKSLFLDAMEDVQPLKRATDVHWHPTRNQRAPQRIDTLQL
                     DNFLTTGFLDIIPLSQPLEFRREGLQHGVLDKLRSGKYPQQASLNLLRQPVEECRKMM
                     FSFIQQAMADGLRNVLIIHGKGRDDKSHANIVRSYVARWLTEFDDVQAYCTALPHHGG
                     SGACYVALRKTAQAKQENWERHAKRSR"
     gene            complement(8087..8995)
                     /locus_tag="RH057_003081"
     CDS             complement(8087..8995)
                     /locus_tag="RH057_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3129416.1"
                     /translation="MAFQVKIHQIRAFVEVARQGSIRGASRMLNMSQPALSKSIQELE
                     EGLAAQLFFRRSKGVTLTDAGESFYQHASLILEELRAAQEEIRQRQGQLAGQINIGMG
                     ASISRSLMPAVISRFHQQHPQVKVRIMEGQLVSMINELRQGELDFTINTYYQGPYDHE
                     FTFEKLLEKQFAIFCRPGHPAIGARSIKQLLDYSWTMPTPHGSYYKQLSELLDDQAQT
                     PQVGVVCETFSACISLVAKSDFLSILPEEMGCDPLHGQGLVMLPVSEILPKAAYYLIQ
                     RRDSRQTPLTASLITQFRRECGYLQS"
     gene            9171..10481
                     /gene="abgA"
                     /locus_tag="RH057_003082"
     CDS             9171..10481
                     /gene="abgA"
                     /locus_tag="RH057_003082"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgA"
                     /protein_id="HDV3129417.1"
                     /translation="MESLNQFVNSLAPKLSHWRRDFHHYAESGWVEFRTATLVAEELQ
                     QLGYSLALGREVVNESSRMGLPDELTLQREFERARQQGALAQWIAVFEGGFTGIVATL
                     DTGRPGPVMAFRVDMDALDLSEEQDVSHRPYRDGFASCNAGMMHACGHDGHTAIGLGL
                     AHTLKQFESGLHGVIKLIFQPAEEGTRGARAMVDAGVVDDVDYFTAVHIGTGVPAGTV
                     VCGSDNFMATTKFDAHFTGTAAHAGAKPEDGHNALLAAAQATLALHAIAPHSEGASRV
                     NVGVMQAGSGRNVVPASALLKVETRGASDVINQYVFDRAQQAIQGAATMYGVGVETRL
                     MGAATASSPSPQWVAWLQSQAAQVAGVNQAIERVEAPAGSEDATLMMARVQQHQGQAS
                     YVVFGTQLAAGHHNEKFDFDEQVLAIAVETLARTALNFPWTRGI"
     gene            10481..11926
                     /gene="abgB"
                     /locus_tag="RH057_003083"
     CDS             10481..11926
                     /gene="abgB"
                     /locus_tag="RH057_003083"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate hydrolase subunit AbgB"
                     /protein_id="HDV3129418.1"
                     /translation="MQEIYRFIDDAIEADRQRYTDIADQIWDHPETRFEEFWSAEHLA
                     SALESAGFTVTRNVGNIPNAFIASFGQGKPVIALLGEYDALAGLSQQAGCAQPTSVTP
                     GENGHGCGHNLLGTAAFAAAIAVKKWLEQYGQGGTVRFYGCPGEEGGSGKTFMVREGV
                     FDDVDAALTWHPEAFAGMFNTRTLANIQASWRFKGIAAHAANSPHLGRSALDAVTLMT
                     TGTNFLNEHIIEKARVHYAITDSGGISPNVVQAQAEVLYLIRAPEMTDVQHIYDRVAK
                     IAEGAALMTETTVECRFDKACSSYLPNRTLENAMYHALSHFGTPEWNSEELAFAKQIQ
                     ATLTPNDRQNSLNNIAATGGENGKVFALRHRETVLANEVAPYAATDNVLAASTDVGDV
                     SWKLPVAQCFSPCFAVGTPLHTWQLVSQGRTSIAHKGMLLAAKTMAATTVNLFIDSGL
                     LQECQQEHQQVTDTQPYHCPIPKNVTPSPLK"
     gene            11963..13489
                     /gene="abgT"
                     /locus_tag="RH057_003084"
     CDS             11963..13489
                     /gene="abgT"
                     /locus_tag="RH057_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-aminobenzoyl-glutamate transporter"
                     /protein_id="HDV3129419.1"
                     /translation="MSMSSIPSSSQSGKLYGWVERIGNKVPHPFLLFIYLIIVLMVTT
                     AILSAFGVSAKNPTDGTPVVVKNLLSVEGLHWFLPNVIKNFSGFAPLGAILALVLGAG
                     LAERVGLLPALMVKMASHVNARYASYMVLFIAFFSHISSDAALVIMPPMGALIFLAVG
                     RHPVAGLLAAIAGVGCGFTANLLIVTTDVLLSGISTEAAAAFNPQMHVSVIDNWYFMA
                     SSVVVLTIVGGLITDKIIEPRLGQWQGNSDEKLQTLTESQRFGLRIAGVVSLLFIAAI
                     ALMVIPENGILRDPINHTVMPSPFIKGIVPLIILFFFVVSLAYGIATRTIRRQADLPH
                     LMIEPMKEMAGFIVMVFPLAQFVAMFNWSNMGKFIAVGLTDILESSGLSGIPAFVGLA
                     LLSSFLCMFIASGSAIWSILAPIFVPMFMLLGFHPAFAQILFRIADSSVLPLAPVSPF
                     VPLFLGFLQRYKPDAKLGTYYSLVLPYPLIFLVVWLLMLLAWYLVGLPIGPGIYPRLS
                     "
     gene            13500..14015
                     /gene="ogt"
                     /locus_tag="RH057_003085"
     CDS             13500..14015
                     /gene="ogt"
                     /locus_tag="RH057_003085"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HDV3129420.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLSDYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            14210..14962
                     /gene="fnr"
                     /locus_tag="RH057_003086"
     CDS             14210..14962
                     /gene="fnr"
                     /locus_tag="RH057_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HDV3129421.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            15114..16064
                     /gene="uspE"
                     /locus_tag="RH057_003087"
     CDS             15114..16064
                     /gene="uspE"
                     /locus_tag="RH057_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HDV3129422.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(16114..16371)
                     /locus_tag="RH057_003088"
     CDS             complement(16114..16371)
                     /locus_tag="RH057_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
                     /protein_id="HDV3129423.1"
                     /translation="MIMAKLKSAKGKKFLFGLLAVFIIAASVVTRATIGGVIEQYNIP
                     LSEWTTSMYVIQSSMIFVYSLVFTVLLAIPLGIYFLGGEEQ"
     gene            16615..>16774
                     /locus_tag="RH057_003089"
     CDS             16615..>16774
                     /locus_tag="RH057_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel protein MscS"
                     /protein_id="HDV3129424.1"
                     /translation="MIAELFTNNALNLVIIFGSCAALILMSFWFRRGNRKRKGFLFHA
                     VQFLIYTII"
BASE COUNT         4263 a         4178 c         4093 g         4240 t
ORIGIN      
        1 atctgtatgg caaagttgat ggcctgcatt atttttctga taattcagcg aaagatggcg
       61 accagagcta tgcgcgtctg ggttttaaag gcgaaaccca aattaacgat caactcactg
      121 gctacggtca atgggaatac aatattcagg caaacaacac tgaatcttca aaaaaccagt
      181 catggacccg tctggcattt gccgggctga aatttgcaga ttacggttct ttcgattacg
      241 gacgtaatta tggcgtaatg tacgacatcg aaggctggac cgatatgctg cctgaatttg
      301 gcggtgactc ttataccaat gcagacaact ttatgactgg tcgagccaat ggcgtcgcga
      361 cttatcgtaa tactgatttc ttcggtctgg taaatggtct gaacttcgcg gtgcagtatc
      421 aaggtaacaa cgaaggagcc agtaatggtc aggaaggcac caacaacgga cgtgatgttc
      481 gccatgaaaa cggtgacggc tggggtcttt ccacaacata tgatttaggc atgggcttta
      541 gcgctggtgc ggcatacacc tcttctgacc gcaccaatga ccaggttaac catactgcgg
      601 cgggtggtga taaagcagac gcgtggactg ctgggctaaa atacgatgct aacaatattt
      661 acctggcaac catgtattca gaaacgcgta atatgacccc gtttggcgac agcgattatg
      721 ctgttgcaaa caaaacccag aactttgaag tcactgcaca gtaccagttt gattttggtc
      781 tgcgtccggc agtctctttc ctgatgtcta aaggccgtga cctgcacgct gccggtggtg
      841 cagacaaccc ggcaggtgtt gatgataaag atctggttaa atacgccgat gttggcgcga
      901 cttactattt caataaaaac atgtccactt atgttgacta taaaatcaac ctgttggatg
      961 aagatgacag tttctacgct gccaatggca tctctaccga tgatattgtc gctttaggtc
     1021 tggtttatca gttctaaatc ctcctgcccg ctgttatggc gggctttttc tgcttattct
     1081 tcctcctttg atctaaatta aaaatgtgaa ctccgtcatt acacaaaaag tgtcatctgg
     1141 cgttacactt tatgcagata ctaaaacagg aggttttatg aacagaacga ttcttgtccc
     1201 tatcgatatt tccgattcag aattaactca acgcgtgatt agccacgttg aggcagaggc
     1261 aaagattgat gatgcagagg ttcatttcct gacggtaata ccttcactgc cctactatgc
     1321 ctctctgggt ttagcgtatt ccgcagaatt accggcaatg gatgacctga aagcggaagc
     1381 caaatcgcaa ctggaagaga tcattaaaaa atttaaactg ccaaccgaca gagtgcatgt
     1441 ccatgttgag gaaggctcgc ccaaagaccg cattctggaa atggcgaaga agatccccgc
     1501 tcatatgatc atcattgctt cccatcgacc ggatatcacc acttatctgc tcggttccaa
     1561 cgccgcagct gtagtgcgtc acgcagagtg ctccgtgctg gttgtgcgct gacactaacg
     1621 cccgcacatt gctgcgggct ttttgattca tttcgcaaat gtgctgacat tttcccctct
     1681 aatccgtacc atacacgcca ctgtttttat atcagatttc ttatgctggg cattccggca
     1741 tgacgcatac tcttctgatg ccatataacg aattgagtcg cttttaaatg tcgcaaaatc
     1801 aagaaattag taagaaagaa caatacaacc tgaacaaatt acaaaaacgt ctgcgtcgta
     1861 acgtgggcga agccattgct gacttcaata tgattgaaga aggcgatcgc atcatggttt
     1921 gcctctccgg gggtaaagac agctatacca tgctggagat tctgcgcaat ttgcagcaaa
     1981 gcgcgccaat caatttttcg ctggtggctg ttaacctcga tcaaaagcaa ccgggcttcc
     2041 cggaacacgt tctgcccgag tatcttgaaa agctgggcgt tgagtacaag attgttgaag
     2101 agaatactta cggtatcgtg aaagagaaga ttccagaggg caaaaccact tgctcactgt
     2161 gttctcgcct tcgtcgcggt atcctttatc gtaccgcaac ggaactgggg gcgacgaaga
     2221 tcgcgttggg tcaccatcgt gacgatatcc tgcaaacgtt gttcttaaat atgttctacg
     2281 gcggcaagat gaaaggtatg cctccgaaac tgatgagcga tgatggcaaa catatcgtta
     2341 ttcgtccgct ggcctactgc cgcgagaaag atattcagcg atttgccgat gcaaaagcgt
     2401 tcccgattat tccgtgcaac ctgtgcggtt cacagcctaa cctgcaacgt caggtgattg
     2461 ctgacatgtt gcgtgactgg gataaacgtt atccagggcg tatcgagacg atgttcagcg
     2521 cgatgcagaa tgtggtgccg tcgcatctgt gcgataccaa cctgttcgat tttaaaggca
     2581 ttacccacgg ttctgaagtg gttaacgggg gtgatctggc gtttgatcgc gaagagatcc
     2641 cactacaacc ggcgggctgg cagccagaag aagatgaaaa tcagttggat gagttacgac
     2701 tgaatgtggt tgaagtgaaa taaccaggat agcgcccgat gcgcaagcgt atcgggctac
     2761 tcttatggag gccggataag acgcggccag cgtcgcatcc ggcaatcccg aataagatgt
     2821 ttactcttgc acccggcaat tcaacatttc attattttaa taaccgcacc cggcacgttt
     2881 ttcctttaat cttcccgccc tgtaactgtt tccatgcttt atgagcaaca gcctgacgga
     2941 ccgcgacata gacatgcgcc ggatgcacgg cgattttgcc aatatctgcg ccatcaagcc
     3001 cgatatctcc tgtcagtgca cctaatacat cacccgggcg cattttggct tttttcccgc
     3061 catcgataca caacgttgcc atttctgctt ccagcggcac aatggaacta ttagctggcg
     3121 gcgtttgcca gttaagtttt atctgcaaca tgtcagaaat gatattggcc cgctgtgctt
     3181 cttccggagc acagaaactg atcgccagac cgctatttcc tgcacgagct gtacgaccga
     3241 tgcgatgtac atgaacttca gggtcccacg ccagctcaaa gttcaccacc agctcaagcg
     3301 atttaatatc cagaccacgc gcagcaacat cagtagcaac cagtacacgg gcgctaccgt
     3361 tagcaaaacg taccagagtc tgatcgcgat cgcgttgctc cagatcgccg tgtaacgaca
     3421 atgcactttg ccctacttca ttcagcgcgt cgcagacagc ctggcaatct tttttggtat
     3481 tgcaaaacac cacgcaagag gatggctgat gcaagcttaa taaccgttgc aacagaggaa
     3541 ttttgccttt gctggatgtc tcataaaatt gttgttcaat gggtggcaaa gcatctgttg
     3601 agtcaatttc aatcgccaaa ggatcgcgtt gtactcgtcc gctgattgcg gcgatggctt
     3661 ccggccaggt tgccgaaaac agaagcgtct gtcgagatgc aggcgcaaaa cggatgacat
     3721 catcaatggc atcgctaaat cccatatcca gcatgcggtc ggcctcatcc atcaccaaag
     3781 tattcagcgc atccagtgat accgtgcctt tttgcaagtg atccagcaaa cgacccggcg
     3841 ttgccacgat aatatgcggc gcatgttgca acgaatcacg ctgcataccg aacggttgac
     3901 caccgcacaa cgtcaaaatt ttggtatttg gcagaaaacg cgccagccga cgcaattcac
     3961 ctgccacctg atccgccagt tcacgcgtag gacacagcac taaagcctgg gtttgaaata
     4021 gcgacgcatc aatttgctgt aacaagccga ggccaaaagc cgccgttttg ccgctgccgg
     4081 ttttcgcctg cacgcgaaca tcttttccgg caaggatcgc cggaagcgcg gcggcctgca
     4141 ccggcgtcat ggttaaataa cccaactcat taaggttcgt gagttgggcg ggaggcaaaa
     4201 cattcagggt agaaaaagcg gtcacaatct attctcgtgg tcatcgacgc aaagttagca
     4261 ggcgcgtatc ctcgcagatc tacgctcacg atgcgacaat ttaatcggtt cttcatcggg
     4321 tgatgggtca ggcatgggtt gcgggcgagg gatcggatcg ggcactggaa caggatcgcc
     4381 aggaatcggt tcagggacag gaatttgcaa ataaataagt gtcgtcatat ttccctccgg
     4441 tcattgggtg gactcttaaa gggtagacgc tgataaataa caggcaaaaa aaagccgact
     4501 catcaaagtc ggcgtcgtac gaatcaattg tgctatgcag taattcaaaa aaggaagtaa
     4561 gacaatatgg agcgcaacgc ccatcgcttg acgttgcatt cacctgcaag ggagatattg
     4621 ccctgaatgg gtagagagtt tattgacttc gctcaaactt tgcggcgttt ttgtatacag
     4681 acagccggaa aaattgcttt tgttacaacc atttactacg gtgcaaccat aaagcaacac
     4741 caccaataag aacaactaac agaatacaaa aaattgaaaa tccgaattgc cacccgccgc
     4801 cagggatccc accaaggttg acgccaaata accctgtcag aaaggtactg ggtaaaaaga
     4861 ccattgccat caatgacatt gtataggtac gacgagctaa attttcctgc atcacctgag
     4921 cgatttcatc cgccatcaca ccagtccgtg ctatacaggc gtcgatttcg tcaaggccgc
     4981 gcccaaggcg atcggcaata tcctgcatcc gacggcgttg gtcatcgctc atccacggca
     5041 aacgttcact ggcaagacga gcatagacat cacgttgcgg tgccatatag cgacgcatca
     5101 cgattaattg tttgcgcagc agagccagga atccacgcgg tggaatttgc tgatcaagga
     5161 gattatcttc aaggtcgata attttatcgt gcagctgctc gataaattcg ctggaatgat
     5221 cggtcaacgc atcgcacaca tccaccagcc accctccgca atcggtcgga cccgtgccct
     5281 cttccagatc gctcaccaca tcgtccagcg ccagcacttt gcgttgtcgg gtcgaaacaa
     5341 ttaaccgccc gtccatatat acacgcatgg cgaccagttg atcggggcgt tcatcggtgc
     5401 tgccgtttat acagcgcaat gtaataagcg tgccttcacc gagacggctg actcggggcc
     5461 gtgtgctctc gcccgccagc gcatcacgta cgttattggg aagcagcggt gttgtcgcca
     5521 gccattgggc gctatcatgg tgtacataat taaggtggag ccagcaggga tgcgcttcat
     5581 caatcacatc tgtattttcc agcggtttaa cgccgcctct accatccagc atccaggcaa
     5641 atactgcatc cgggacatta acgtccgatc ccttaatcgc ttccacagtg cctccatcat
     5701 caacgcatta ttttgtagtc tagccttctg gccctgttac gcaacatctc atcaccccat
     5761 taccctgaaa tgattaataa aattctgtct aaattgaata caaaaagcaa aatgcttttc
     5821 cgtatacaaa ccgtgtgaag tgttaaatag cgtctatcat tatcagaatt atctgatcat
     5881 atgacgtggc ttttttgcga tcggatagca acaaaaattg ataaaaataa tgggatctca
     5941 atgattacgc acaacttcaa taccctggac ttactcacca gtcctgtctg gatcgtttcg
     6001 ccctttgagg aacagttaat ttatgccaat agcgcggcgc gactgttgat gcaagacctc
     6061 acgtttagtc agctacgaac cggaccctat tccgtctcct cacaaaaaga actgccgaaa
     6121 tacctctccg atctgcaaaa ccaacacgat attatcgaaa tcctcactgt tcagcgtaaa
     6181 gaagaggaaa cagcattaag ctgtcggctt gttttgcgag agctgacaga aacagaaccg
     6241 gtgattattt tcgaaggtat cgaagcgccg gcaacgctgg gtttaaaagc cagtcgctcg
     6301 gcaaattatc agcgcaaaaa acaaggtttt tatgcgcgct tttttctgac taactctgca
     6361 ccaatgttgt tgattgaccc ctcacgagat ggccaaatcg tcgatgctaa cctcgccgcg
     6421 ctcaatttct atggttataa ccatgaaacg atgtgccaga aacatacctg ggaaattaat
     6481 atgctcgggc gtcgcgtcat gcctatcatg catgaaattt cgcatttacc cggcggtcat
     6541 aaacctttga attttcttca taaactggcg gatggatcta ctcgtcatgt gcagacctat
     6601 gccgggccga ttgaaattta tggcgacaag ctcatgttat gtattgtgca tgatattact
     6661 gagcaaaaac ggctggagga gcagctggaa catgctgctc accatgacgc gatgaccgga
     6721 ttactgaatc ggcgacagtt ttatcacatt accgagccag gccaaatgca gcacctcgcc
     6781 atcgcccagg attacagctt gttgctcatc gacaccgatc gttttaaaca cattaacgat
     6841 ctctatgggc attctaaagg tgatgaggtg ttatgcgccc tcgcccgcac cctcgaaagt
     6901 tgcgctcgca aaggcgattt ggtgtttcgt tggggaggcg aagagtttgt cttattgcta
     6961 ccaagaaccc cactggatac cgcgctttcg ctggctgaaa ctatccgtgt aagcgtggca
     7021 aaagtgagta tttcgggctt accacgtttt accgtcagca ttggtgtggc gcatcacgaa
     7081 ggaaatgaaa gcatcgatga actgtttaaa cgcgttgatg atgctttgta tcgggcgaaa
     7141 aatgatggac gcaaccgcgt gctggcggca taagccgcgg atgcgtctcg agatcaacgg
     7201 ctgcgcttag cgtggcgctc ccagttttct tgcttcgcct gcgccgtttt acgtagtgcg
     7261 acgtaacacg ccccgctgcc gccatgatgc ggtagcgcgg tgcaatatgc ctgaacatca
     7321 tcaaattcgg tcagccagcg cgccacatag ctgcggacaa tattggcatg cgatttatca
     7381 tcccgtcctt tgccatgaat aatcagcacg ttacgcaaac catcagccat ggcttgttga
     7441 atgaaactga acatcatttt gcggcactct tccaccggct ggcgcaaaag attcaggctc
     7501 gcctgttgcg gatatttacc actgcgcagc ttatccagca ccccatgttg caacccttcc
     7561 cgccgaaact ccagcggctg acttagtggg atgatgtcga gaaatccggt ggtgaggaaa
     7621 ttatcaagct gcagcgtgtc gatacgctgc ggcgcacgtt ggttacgcgt tggatgccag
     7681 tggacatcgg tagcacgttt cagcggctgg acatcttcca tggcgtcaag aaacagcgat
     7741 ttgtcgtcaa ggttcatgtt acatcctccc gcaattaaga gcgcgatatg ataaccagac
     7801 cgggtcggtc caacaacgta ttacccaaaa ttccagtaat aagttccaaa tattgccgat
     7861 attttaagca aaatgcttat gcatgattat tcattcacga tattaataat gtaacttata
     7921 ttttcgtgaa atctgtcact gaagaaaatt ggcaactaaa ggttaaaacc gttataacac
     7981 agtcaccggc gcagaggaga caatgccgga cttaagacgc ggatgcactg ctgtgtgtac
     8041 tgtagagtct ggcggatgtc gacagactct atttttttat gcagttttaa ctttgcagat
     8101 agccgcattc tcgccggaat tgcgtgatta atgacgcggt cagtggtgtc tggcgactat
     8161 cacgccgctg aatcaaataa taggccgctt tcggtaaaat ttcgctaacc ggcaacatca
     8221 ccagcccctg tccgtgcaag ggatcgcagc ccatttcttc aggcagtatg ctgagaaaat
     8281 cgctttttgc caccagactg atacaggctg agaacgtctc gcagactaca ccgacctgtg
     8341 gcgtttgcgc ctgatcgtca agcaattcac tcaactgttt gtagtaactg ccgtgtggcg
     8401 tcggcattgt ccagctgtaa tccagtaact gtttgatcga acgggcacca atggcggggt
     8461 gtcccgggcg gcaaaagatc gcgaattgct tttccagtaa tttctcaaaa gtaaattcgt
     8521 ggtcgtacgg tccctgataa taggtattga tggtgaaatc caattctccc tgacgcaatt
     8581 cattaatcat cgacaccagt tgcccttcca taatgcgtac ttttacctgc ggatgctgct
     8641 gatgaaaacg agatatgaca gctggcatca gactgcggga aatactggcc cccatgccga
     8701 tattaatctg ccctgccagt tgcccttgtc gttggcgaat ttcctcttgc gctgcgcgca
     8761 gctcttcaag aataagactg gcgtgctgat aaaaactttc accggcatca gttaacgtca
     8821 cgcctttact acggcgaaaa aagagttgcg ccgctaatcc ttcttccagc tcctgaatag
     8881 atttactcag tgccggttgc gacatattca acattcggct cgctccgcga atgctgccct
     8941 gacgagccac ttcaacaaaa gcacgaattt gatgaatttt tacctgaaaa gccatgacgc
     9001 caccgataac cgttatttat cagaccaaag aaactggcat ctactttaat gcagatgatt
     9061 gtgtcagggt aatttatgaa cggttaaaac tgtgaaaaat cagttagtga taagtaaaaa
     9121 ctatcgctac gtgaaccggg tcacactttt tactgatgac gggaaaggtt atggagtctt
     9181 tgaatcaatt tgttaattcg cttgccccaa aattatcgca ctggcgacgt gattttcatc
     9241 actatgcaga gtctggctgg gtggaattcc gcactgccac ccttgttgcg gaagaattgc
     9301 agcagctcgg ctattcactg gcgctgggcc gcgaagtagt taatgaaagt agccggatgg
     9361 gactacctga tgaactcact ctacaacgcg aattcgagcg cgctcgtcaa cagggtgcgc
     9421 tagcacaatg gattgcggtt tttgaaggtg gtttcactgg tatcgtcgcc accctggata
     9481 ccggtcgccc cggtccggtg atggctttcc gtgtcgatat ggacgcgctg gatctcagtg
     9541 aagagcagga tgtcagccat cgcccctacc gcgacggttt tgcgtcatgt aacgccggaa
     9601 tgatgcatgc ctgtggtcat gatggacata ccgccattgg gcttgggctg gcgcataccc
     9661 ttaaacaatt cgagtccgga ctacatggcg tcatcaaact gatttttcag cctgcagagg
     9721 aaggtacgcg tggcgcgcgg gcgatggtcg atgcaggtgt cgtagatgat gttgattatt
     9781 ttactgccgt gcacattggc actggcgtac ctgcgggcac cgtggtgtgc ggcagtgata
     9841 attttatggc aaccaccaaa tttgacgcgc acttcaccgg gaccgccgct cacgcaggcg
     9901 caaaaccaga agacggtcac aatgccttgt tggcggcagc acaagccact cttgcactgc
     9961 atgcaatcgc cccgcacagc gaaggagctt ccagagtaaa cgtgggcgtt atgcaggcag
    10021 gaagcggtcg taacgttgtt cctgcctcgg cgttgctgaa agtggaaaca cgcggggcca
    10081 gcgacgtcat taatcaatat gtttttgacc gtgcacaaca agcgattcag ggcgcagcaa
    10141 ccatgtatgg tgtcggcgtt gaaactcgtc tgatgggtgc agctaccgcc agttctcctt
    10201 cgccgcaatg ggtcgcatgg ttgcaaagtc aggcggctca ggtcgcgggg gtcaatcagg
    10261 ccattgaacg tgttgaagcg cctgcgggtt ccgaagatgc cacattaatg atggcccgcg
    10321 tgcagcaaca tcaagggcaa gcctcctacg tggtgtttgg cacacagctg gcggcaggtc
    10381 atcacaacga aaaattcgat tttgacgagc aggttctcgc tattgccgtc gaaacgctgg
    10441 cgcgcaccgc gctcaatttt ccctggacgc gaggtatctg atgcaggaaa tctatcgttt
    10501 tatcgacgat gcgattgaag ccgatcgcca acgttatacc gatattgccg atcaaatctg
    10561 ggatcatcca gaaacacgtt ttgaagagtt ctggtcagcg gagcatctgg cttcggcgct
    10621 ggaatctgca ggcttcaccg ttacccgcaa cgtaggcaat atcccaaatg cctttattgc
    10681 ttcgtttggt caaggcaaac cggttatcgc cctgctggga gaatatgacg ccctggcagg
    10741 tttaagtcag caagcaggtt gcgcgcaacc tacatccgtg acgcccggtg aaaatggtca
    10801 cggttgcgga cacaatttgc tgggaaccgc cgcctttgcc gctgcaatag ccgtcaagaa
    10861 atggctggaa caatatgggc aaggcggcac agtgcgcttt tatggttgtc ctggcgaaga
    10921 aggcggctcg ggtaaaacgt tcatggtccg cgagggggta tttgatgatg tggatgcggc
    10981 actcacctgg cacccggaag cctttgccgg tatgttcaat acccgcacgc tggcaaacat
    11041 tcaggcatca tggcgcttta aagggatcgc agcacatgcc gcgaattccc ctcatttggg
    11101 acgaagcgcc cttgatgccg taacgttgat gaccactggc accaacttcc tcaacgaaca
    11161 tattattgaa aaagcgcgcg tacactatgc catcacagat agcggcggga tctcgcccaa
    11221 cgtggtccag gcgcaggcag aagtgcttta tcttatccgt gcccccgaaa tgaccgacgt
    11281 gcagcatatt tatgatcggg tcgccaaaat cgccgaaggt gcggcattga tgaccgaaac
    11341 cacggttgaa tgccgcttcg acaaagcctg ttccagttat ctcccgaatc gcaccttaga
    11401 aaatgccatg taccatgccc tatcccattt tggtaccccg gaatggaact ccgaagaact
    11461 ggcttttgcg aaacaaattc aggctacgct cacccccaac gatcggcaaa acagtctgaa
    11521 taatatcgcc gcaaccggtg gcgaaaacgg caaggttttt gcactacgtc atcgtgaaac
    11581 ggtactggcg aatgaggtcg ctccatatgc cgccaccgat aacgtgcttg cggcatcgac
    11641 tgatgtcggc gacgtcagtt ggaaactgcc tgttgcccag tgtttcagcc cctgttttgc
    11701 cgtcggtaca ccgctacata cgtggcaact ggttagccag gggcgaacat ctattgctca
    11761 taaaggaatg ctgctggcgg cgaaaactat ggcagcaacc acagtcaatc tcttcattga
    11821 ttcagggcta ttgcaagaat gccaacaaga gcatcagcaa gttacggaca cgcaaccgta
    11881 tcactgccct atcccgaaaa acgtgacacc gtcaccttta aaataacaac aacaacgcaa
    11941 acacaacaac cgaggaatgc ccatgagtat gtcatccata ccgtcgtcct cccaatccgg
    12001 gaagctctat ggctgggtcg aaagaattgg taacaaggtt ccccatcctt ttttgctctt
    12061 tatctatttg ataatcgtac tcatggtgac gacggcaatt ttgtcagcct ttggcgtcag
    12121 tgcgaaaaac ccgaccgatg gtacgccggt ggtggtgaaa aacctgctca gtgtggaagg
    12181 attacactgg tttttaccca atgtgattaa aaactttagc ggttttgctc cacttggagc
    12241 gatcctggcg ctggttttag gtgccggcct agcggagcgc gtcggcttac tgccagcgct
    12301 aatggttaaa atggcatcgc atgttaatgc ccgctacgcc agttatatgg tgctgtttat
    12361 tgcttttttc agccacattt cttccgatgc ggcgttagtg atcatgccac cgatgggtgc
    12421 gctgattttt ctggcggtgg gcaggcatcc agttgcaggt ttactggctg ccattgcagg
    12481 cgtaggttgc ggctttacgg ctaatttact gattgtcaca accgacgtgt tgctgtcggg
    12541 gatcagcacg gaagcggcgg ctgcgttcaa tccgcaaatg cacgtcagtg taattgataa
    12601 ctggtatttt atggccagct ccgtagtcgt actgacgatt gttggcggcc tgataaccga
    12661 caaaatcatc gagccacgat taggtcaatg gcagggaaac agcgatgaga aactgcagac
    12721 attgaccgaa agtcagcgtt ttggtttacg catagcaggt gtcgtatcgc tactttttat
    12781 tgctgcgatc gcgctgatgg tgatcccgga aaacgggata ttgcgcgatc cgattaatca
    12841 caccgtgatg ccatcaccct ttattaaagg tatcgtgcca ctgatcattc tttttttctt
    12901 tgttgtctcg ctggcttatg gcatcgctac ccgcacaatt cgacgtcagg cggatttacc
    12961 gcatttaatg attgaaccga tgaaagagat ggcgggattt atcgtgatgg tttttcccct
    13021 cgcccaattt gtcgccatgt ttaactggag caacatgggg aaattcatcg ccgtggggct
    13081 gaccgatata ctggaaagtt cagggcttag cggcataccg gcgtttgtcg gtctggcgtt
    13141 gctttcctct ttcttatgca tgtttattgc cagcggttcc gcaatctggt cgattctggc
    13201 ccccattttc gtaccaatgt ttatgctact tggctttcac ccggcatttg cgcaaatcct
    13261 ctttcgtatt gccgactcat ccgtattgcc tttagcgccg gtatctcctt ttgtcccact
    13321 gtttcttgga ttcctgcaac gctacaaacc agacgcgaaa ctgggtactt actattcgtt
    13381 agtcttgccc tatccgctta tctttttggt ggtatggctg ctgatgttgc tggcgtggta
    13441 tcttgttggt ctgccgatag gtccggggat ttacccacgt ttgtcttaag agagaacgga
    13501 tgctgagatt acttgaagaa aaaattgcca cgccactggg tccactgtgg gtgatttgcg
    13561 atgagcaatt tcgcctgcgg gcggttgaat gggaagagta cagcgaacgc atggtgcagc
    13621 tgctggacat ccattatcgc aaagaaggct atgagcgcat ttctgccacc aacccaggcg
    13681 gtttaagcga caagcttagt gattattttg ccggtaatct tagcattatt gatacgcttc
    13741 ccactgccac aggggggacg ccatttcagc gcgaagtctg gaaaactcta cgcactatcc
    13801 cctgcgggca ggtaatgcat tacggccaac tggctgaaca attgggccgc cctggcgcgg
    13861 cgcgtgccgt tggtgcggca aacggatcga atcccatcag catcgtcgta ccttgccatc
    13921 gggttattgg ccgaaacggc accatgaccg gatatgcagg cggagttcag cgaaaagagt
    13981 ggttattgcg ccacgaaggt tatcttttgc tgtaaacatt aaacaatttg tgccagcttg
    14041 ttcacacttt tatgtaaagt tacccttaac aacttaaggg ttttcaaata gatagacata
    14101 tatttacatc tgatatcgga attctctgct gttaaggttt gcttagactt acttgctccc
    14161 taaaaagatg ttaaaattga caaatatcaa ttacggcttg agcagaccta tgatcccgga
    14221 aaagcgaatt atacggcgca ttcagtctgg cggttgtgct atccattgcc aggattgcag
    14281 catcagccag ctttgcatcc cgttcacact caacgaacat gagcttgatc agcttgataa
    14341 tatcattgag cggaagaagc ctattcagaa aggccagacg ctgtttaagg ctggtgatga
    14401 acttaaatcg ctttatgcca tccgctccgg tacgattaaa agttatacca tcactgagca
    14461 aggcgacgag caaatcactg gtttccattt agctggcgac ctggtgggat ttgacgccat
    14521 cggcagcggc catcacccga gcttcgctca ggcgctggaa acctcgatgg tatgtgaaat
    14581 cccgttcgaa acgctggacg atttgtccgg taaaatgccg aatctgcgtc agcagatgat
    14641 gcgtctgatg agcggtgaaa tcaaaggcga tcaggacatg atcctgctgt tgtcgaagaa
    14701 aaatgccgag gaacgtctgg ctgcattcat ctacaacctg tcccgtcgtt ttgcccaacg
    14761 cggcttctcc cctcgtgaat tccgcctgac gatgactcgt ggcgatatcg gtaactatct
    14821 gggcctgacg gtagaaacca tcagccgtct gctgggtcgc ttccagaaaa gcggtatgct
    14881 ggcagtcaaa ggtaaataca tcaccatcga aaataacgat gcgctggccc agcttgctgg
    14941 tcatacgcgt aatgttgcct gatttttccg cataactcac tatccttctg tcatatcatt
    15001 aaatttttct gatttattga tctggcagaa ggttcatcac tgtttcattc accagatatg
    15061 ggttaatctt ttaattacaa actgcgttga cagttgttgt aaggagaccc tgtatggcta
    15121 tgtatcagaa catgctcgtt gttatcgatc ctaaccagga cgaccaacca gcattgcggc
    15181 gagctgttta tttacatcaa cggattggtg gcaaaattaa agcctttttg ccgatctatg
    15241 acttctcata cgaaatgacc accctgctct ccccggacga acgtacggct atgcgtcagg
    15301 gcgtcatcag ccagcgtaca gcctggatcc acgagcaggc aaaatattat ctcaatgctg
    15361 gcgttcccat tgaaattaaa gtggtctggc ataaccgtcc tttcgaagcc atcattcagg
    15421 aagtgatcag cggcgggcac gatttggtgc taaaaatggc gcaccaacat gaccgtctgg
    15481 aagcggtgat ttttacgcca acggactggc atctgttacg caaatgccca agcccggtgt
    15541 ggatggtgaa agaccagccg tggccggaag gaggcaaggc gctggtggcg gtgaatctcg
    15601 ccagtgaaga gccgtatcat aatgcgctca atgaaaaact ggtcaaagag acgatcgaac
    15661 tggcagaaca agtcaaccat accgaagttc atctggttgg cgcttatccg gtaacgccaa
    15721 ttaatatcgc gattgaactg ccggaatttg atccgagcgt ttataacgat gccattcgtg
    15781 ggcaacattt gctggcaatg aaagctctgc ggcagaaatt cggcattaat gaaaacatga
    15841 cgcacgtaga aaaaggtctg ccagaagagg tgattcctga tttggcggaa cacttacagg
    15901 cggggattgt ggttctgggc acggtaggac gcaccggtat ttcagcagca ttcctcggca
    15961 acacggcgga acaggtgatt gatcatcttc gctgcgatct gttggtcatt aaacctgacc
    16021 agtatcagac acccgttgaa ctggatgacg aagaagacga ttaacgactc ttcaataact
    16081 gacaaggccc gagttatcgg gcctattttt tacttactgc tcttcgccgc caaggaaata
    16141 aattcccaac gggattgcca gcaacacagt aaagaccagg ctataaacaa aaatcatcga
    16201 tgactgaatc acatacattg atgtcgtcca ctcagacagc ggaatattgt actgttctat
    16261 aacgccgccg atagtcgcgc gagtcacaac cgacgccgca ataatgaaaa ccgccaacaa
    16321 accaaagaga aatttcttcc ctttcgctga cttcagtttc gccataatca tgatgtaatc
    16381 cttgtgcaga caaatttctt gttacctaaa cgtaacaata ccatgacatt tcaccgttgt
    16441 ctgctctgaa aacctcacct cttagctgga tttacaatca acgcattgat tagttagcta
    16501 atgttgctac ttccttccct gactattgat gatctgaatg aattgtcgcg gtaaaatagc
    16561 cccgccaggg gtttccggca tgacgacaat tatctggagg agcctgataa aacaatgatc
    16621 gctgaactgt ttacaaataa tgcgcttaat ctggtcatta ttttcggtag ctgcgcagca
    16681 ttgattctga tgagcttttg gtttcgccgc ggaaatcgta aaagaaaagg ttttttattc
    16741 catgcggtgc aatttttaat ctacaccata atta
//
LOCUS       DAPHDW010000095        16595 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.096, whole genome shotgun sequence.
ACCESSION   DAPHDW010000095 DAPHDW010000000
VERSION     DAPHDW010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.096"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(171..2132)
                     /gene="topB"
                     /locus_tag="RH057_003090"
     CDS             complement(171..2132)
                     /gene="topB"
                     /locus_tag="RH057_003090"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HDV3129425.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKILRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEVGSGAIA"
     gene            complement(2137..3180)
                     /gene="selD"
                     /locus_tag="RH057_003091"
     CDS             complement(2137..3180)
                     /gene="selD"
                     /locus_tag="RH057_003091"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HDV3129426.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYDAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            complement(3297..3848)
                     /locus_tag="RH057_003092"
     CDS             complement(3297..3848)
                     /locus_tag="RH057_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HDV3129427.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGRERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCEENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            4009..5865
                     /gene="sppA"
                     /locus_tag="RH057_003093"
     CDS             4009..5865
                     /gene="sppA"
                     /locus_tag="RH057_003093"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HDV3129428.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRLIPAQQVFPGAQGLLVGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKPVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTIENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            6032..7048
                     /gene="ansA"
                     /locus_tag="RH057_003094"
     CDS             6032..7048
                     /gene="ansA"
                     /locus_tag="RH057_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HDV3129429.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAANYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMGGYATGNALAHAGVIGGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            7059..7700
                     /gene="pncA"
                     /locus_tag="RH057_003095"
     CDS             7059..7700
                     /gene="pncA"
                     /locus_tag="RH057_003095"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HDV3129430.1"
                     /translation="MPPRALLLVDLQNDFCAGGALAVPEGDSTVDVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHCVEPYMPGQLDGLPQTFWPDHCVQNSEGAQLHPLLNQ
                     KAIAAVFHKGENPLVDSYSAFFDNGRRQKTSLDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(7861..8133)
                     /locus_tag="RH057_003096"
     CDS             complement(7861..8133)
                     /locus_tag="RH057_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HDV3129431.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            complement(8175..8588)
                     /gene="msrB"
                     /locus_tag="RH057_003097"
     CDS             complement(8175..8588)
                     /gene="msrB"
                     /locus_tag="RH057_003097"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HDV3129432.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            8930..9925
                     /gene="gapA"
                     /locus_tag="RH057_003098"
     CDS             8930..9925
                     /gene="gapA"
                     /locus_tag="RH057_003098"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HDV3129433.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            10009..10893
                     /locus_tag="RH057_003099"
     CDS             10009..10893
                     /locus_tag="RH057_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HDV3129434.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHREDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(10944..11799)
                     /gene="yeaE"
                     /locus_tag="RH057_003100"
                     /pseudo
     CDS             complement(10944..11799)
                     /gene="yeaE"
                     /locus_tag="RH057_003100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal reductase YeaE"
     gene            complement(11889..12635)
                     /gene="mipA"
                     /locus_tag="RH057_003101"
     CDS             complement(11889..12635)
                     /gene="mipA"
                     /locus_tag="RH057_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HDV3129435.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            13071..15005
                     /gene="yeaG"
                     /locus_tag="RH057_003102"
     CDS             13071..15005
                     /gene="yeaG"
                     /locus_tag="RH057_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HDV3129436.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQEPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNESEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQQIEREQFPQEQAERYLEFLKGYLIPKYAEFIGKEIQTAYLESYSEYGQNIFDRYVT
                     YADFWIQDQEYRDPDTGQLFDRESLNAELEKIEKPAGISNPKDFRNEIVNFVLRARAN
                     NSGRNPNWTSYEKLRTVIEKKMFSNTEELLPVISFNAKTSTDEQKKHDDFVDRMMEKG
                     YTRKQVRLLCEWYLRVRKSS"
     gene            15118..16401
                     /locus_tag="RH057_003103"
     CDS             15118..16401
                     /locus_tag="RH057_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaH/YhbH family protein"
                     /protein_id="HDV3129437.1"
                     /translation="MTWFIDRRLNGKNKSMVNRQRFLRRYKAQIKQSISEAINKRSVT
                     DVDSGESVSIPTEDISEPMFHQGRGGLRHRVHPGNDHFVQNDRIERPQGGGGGSGSGQ
                     GQASQDGEGQDEFVFQISKDEYLDLLFEDLALPNLKQNQQRQLTEYKTHRAGYTANGV
                     PANISVVRSLQNSLARRTAMTAGKRRELHALEENLAIISNSEPAQLLEEERLRKEIAE
                     LRAKIERVPFIDTFDLRYKNYEKRPDPSSQAVMFCLMDVSGSMDQSTKDMAKRFYILL
                     YLFLSRTYKNVEVVYIRHHTQAKEVDEHEFFYSQETGGTIVSSALKLMDEVVKERYNP
                     AQWNIYAAQASDGDNWADDSPLCHEILAKKLLPVVRYYSYIEITRRAHQTLWREYEHL
                     QSTFDNFAMQHIRDQDDIYPVFRELFHKQNATAKD"
BASE COUNT         4091 a         4283 c         4079 g         4142 t
ORIGIN      
        1 acttctcgct cccaggatgg ttttgtaata aaactttttt catcacattc cctgttatta
       61 catactttag aggcgataat tttatccatg caaaaaaaat atccaacgaa ttttcttgat
      121 ccgctggata tttcagaata tgactcgaat agcacgaaag attcactcgc ttacgctatc
      181 gccccgcttc cgacttcatc tgctggcgga ctttttttcg cactacgttt acgcggtgca
      241 gcctttttct tatcagcact gccaccactg cccggagcca caatgccgcg aaactgccgc
      301 accggcgtac gtttggcttg atcaataagc tgatatagcg tccccaccag cggctgcata
      361 aagtcctgat agcgacactg cttttcgctg atttgcgtca gcaccgattc ccagtgcgcg
      421 gtcatgtccg gtcgcgtcgc catttctggc agcgaatgga ataacgcttt tccggcgtcg
      481 gtagagtgga tatagcgccc ttttttggtc agaaaaccac gcttgaacag cagttcaata
      541 atcccggcac gggttgcttc tgtccccaga ccatcggtcg cacgaaggat ctttttcaga
      601 tctttgtcct gcacaaagcg cgcgatcccg gtcatcgccg aaagcagtgt tgcatcggta
      661 aaatggcgcg gcggctgggt ttgccgctct accacttcac ctttttcaca cagcaactca
      721 tcgcctttcg ccaccacagg caatggcgta ccgtcgtttt cttcatcgcg ctctttgctg
      781 cctaacagcg tgcgccagcc tgcttcagca agaaaacggg ctttagcgac aaatttgcct
      841 ttggcaatgt ccagttcgat aacacacttg cggaacaccg catccgggca gaactgcatc
      901 aggtattgac gggcaatcag gttatagacc ttcgcttcgt tctccgtcag gttgatcgca
      961 gaactccgtg cggtcgggat gatggcgtgg tgcgcatcga cctttttgtc atcccaacag
     1021 cggttgcgta tatctggatc taccactggc tgcggcaaca gatctggtgc atgaacgctg
     1081 atggcattca tcaccgcgtg gcgtccggca aaatgttctt ctggcaaata gcgacaatcg
     1141 gaacgcggat aagtgattag cttgtgcgtt tcgtacagct tctggcagat atcaagcacg
     1201 ttctgcgcgc tcagaccaaa gcgttttgcc gcttcaatct gcaacgccga aagcgaaaac
     1261 ggcagcggcg cggattctga ttcccgttta tcgttatagc tggtgacaat agccggttga
     1321 ccgctgatgc ggttaaccac atgctccgcc agtggacgat gtaacaagcg cccttcttca
     1381 tcctggtacg gttcacacgc ttcgctcggt tgccagatag cggtaaaccg ctcatcggca
     1441 ggtgtcacga tatgtgcttt gacttcaaag aagtctttcg ccacgaagtt ttcaatctct
     1501 tcatcgcggc gcaccaccag cccaagcacg ggcgtctgca cgcgtccgac ggaaagcacg
     1561 ccctgataac cggcattgcg accgagaatg gtgtaagcac gggtcatatt gatgccgtac
     1621 agccagtcgg cacgcgctcg cgccagcgca gaaacgcaca gcggtacaaa ctcactgttg
     1681 gaacgaagac ggtcgatcgc ccgctcaacc gcctgcgggt tcaggtcgtt tatcaagcaa
     1741 cgctgcacct gctggcgctt ttccggtgcc agttgcagat agtccagcac ttcatccacc
     1801 agcaattgcc cttcacgatc cgggtccccg gcgtgaacga tttcgctggc ttcatgcagg
     1861 aagcgtttga tgacgttaag ttgtttggtc acggagggtc gtggctgtaa ttgccacttt
     1921 tccgggacaa tcggcaaatc cgcaagattc cagcgcgcat agcggctgtc gtaggcgtct
     1981 ggctgcgcct gctcaagcag gtgaccgata caccaggtca ccacctgacc attaccgcac
     2041 tcgataaagc catcgccttt acggtgcggt ttgggcagga catcagcaat ggcgcgcgcc
     2101 agactcggtt tttcggcaat aaacaaccgc attgaattaa cggatctcaa ccatggcacg
     2161 accgccgcgc gctggcacca gttcgccaat cgccgtcagt tcaatgccaa actcggcggc
     2221 tgtagtttta acctcatttt ctgcttccgg catgaccgcc agcagcaaac cgccagaagt
     2281 ttgcggatcg cacagcagat cgcgcacttc acgcggcatt tcacccatca gatgaccgta
     2341 gctggcaaag ttacgttcgg tgccgccagg cactgcgccc aacttaatgt actcttcaac
     2401 ccccggcaat tttgggatcg cgtcatagtc aacgcgcgcc tgcacacctg cgccctgaca
     2461 catttcgctc aaatggccca gcagaccaaa accagtaacg tcggtcatcg cttttacgcc
     2521 ttcgatgttg gcaaaggacg cacctgcgat gttcatccgg cacatcactt ccgtcgccag
     2581 tccctgatgt tctggtttca acagtgattt tttctcagcc gtggtaagaa cgccgatccc
     2641 cagcggtttc gtcaggaaca gtttgcatcc ggcttgtgcg gtactgtttt tcttcacccg
     2701 ctcggtcggt acgatccccg ttaccgccag accaaaaatc ggctccggcg catcgatgga
     2761 gtgaccgcca gccagcgcaa tacccgcctg acggcatgca tagcgtccac cttcggttac
     2821 ttcgcgggca atttctgggg aaagtttgtt aatcggccag ccgaggatcg caatcgccat
     2881 aatcggtttg ccccccatcg cgaagatatc gctgatggcg ttagtcgccg caatgcggcc
     2941 aaaatcgaaa ggattatcaa cgatcggcat aaagaagtcg gtggtactga taacgctggt
     3001 gccattgccc agatcgtaca ccgccgcatc gtcgcgggtc tcattaccca caagcaaatt
     3061 cggatcaaca aacttcgcct gctcactgtg caggatggtt tccaacactt ttggggaaat
     3121 tttacagccg caaccagctc cgtggctgta ttgggtcaaa cgaatcgagt tctcgctcat
     3181 ggacatctcc tgtcaatgca atccgggtat ggtaaccctc attccgtgaa gtgataagtg
     3241 agagtgtctg aattcctgcg cctttgctca caatccagac agtttcgcga caattatcag
     3301 aaataagtta caaacggagt cgggtccggg acgttaatcg acgtagatgc tttcaactgc
     3361 ggcgtgccga ggtagagaaa accgacaatt ttatcctgct cacggcaacc gaatgcttca
     3421 cgcactaccg gactttcagt taatgcgcca ctgcgccaga tgccgccaaa tccttgggca
     3481 acggctgcca tttgcatcgc catgaccgcg catccggcag acatttcctg ttcccatcgc
     3541 gggactttat gattttcttc gcatttcgcc accaccgtga tgatgagcgg tgcgcggaac
     3601 ggcgcattac gggctttgtc gatagctttg tcatcactac cggcagcaat cgccccctgt
     3661 tccagtacgg cgctgaaacg ctcgcgccct tccccttcaa tcacaaaaaa atgccacggt
     3721 tgcatggact tatggtccgg cgcacgcata cccgcacgca ggatgttttg cagttgttca
     3781 cccgttggcg cgggttcagc caggcgggag gcgctacggc gattgatcaa tagttcgagt
     3841 gcatccattt gattaactcc tgtcgtgata tttattcaca aaattaacac gagagtggat
     3901 tttgttacag cacagtccgc aattcctgct gacaagtgcc ggttgggtca ttacgataac
     3961 cacatctatt gcgcctgtga caggtgtgac cttaagttgg gagaatacat gcgaaccctt
     4021 tggcgattta ttgccggatt ttttaaatgg acgtggcgtc tgctgaattt cgtccgtgaa
     4081 atggtactta acctgttctt tattttcctc gtactggttg gtgtggggat ctggatgcag
     4141 gtcagtggtg gtgattcgaa agaaacggcc agtcgtggcg cactgctgct ggacatttct
     4201 ggtgtgatcg tcgataaacc cgacagttct cagcggttta gtaaattaag ccgccagctg
     4261 cttggtgcca gttccgatcg tctgcaggaa aactcactgt ttgatatcgt caacaccatt
     4321 cgccaggcga aggacgaccg caatatcacc ggtatcgtga tggatctgaa aaacttcgca
     4381 ggcggcgacc agccgtctat gcagtacatc ggcaaagccc tgaaagagtt tcgtgacagc
     4441 gggaaaccgg tttatgccgt tggcgagaac tacagccagg ggcaatatta tctcgccagt
     4501 ttcgccaata aaatttggct gtcaccgcaa ggcgtggttg atctgcacgg ttttgctacc
     4561 aacggtctgt actacaaatc gttgctggat aagctgaaag tttccaccca tgtgttccgc
     4621 gtgggtacgt ataaatctgc cgttgaaccg tttattcgtg atgatatgtc accggcagcg
     4681 cgcgaagctg acagccgctg gattggtgag ctgtggcaaa actatctgaa tactgttgcc
     4741 gctaaccggc tgatccctgc tcagcaggta ttccctggcg cgcaagggtt gcttgtgggt
     4801 ttaaccaaaa ccggtggcga taccgcgaaa tatgcactgg aaaacaagct ggtcgatgca
     4861 ctggcatcga gtgccgaaat cgaaaaagct ctgaccaaag agttcggctg gagtaagact
     4921 gataaaaatt atcgcgccat cagttattac gattacgcat tgaaaacgcc ggcagatacc
     4981 ggtgacagca tcggtgtcgt cttcgctaat ggcgcaatta tggatggcga ggaaactcag
     5041 gggaatgttg gcggtgatac cactgcggca caaatccgcg acgctcgcct tgacccgaaa
     5101 gtgaaagcga ttgtcctgcg tgttaatagc ccaggcggca gcgttaccgc gtctgaagtg
     5161 attcgcgctg aactggcagc agcccgggca gcgggtaagc ctgtggttgt atcgatgggc
     5221 ggcatggcgg catctggtgg ttactggatt tccacgccag ctaattacat tgtggctaac
     5281 cccagcaccc tgaccggttc tatcggtatc ttcggcgtga tcaccaccat agaaaatagt
     5341 ctggattcga ttggtgttca taccgatggt gtctcaactt caccgctggc agatgtttct
     5401 atcaccaggg cactgccgcc ggaagcacag cagatgatgc agttaagcat tgagaatggc
     5461 tataaacgct ttatcacgct ggttgctgat gcgcgtcatt cgacgccgga gcaaattgat
     5521 aaaatcgccc agggccacgt ctggaccggt caggatgcaa aagctaacgg gctggtcgat
     5581 agtctcgggg atttcgatga tgcggttgcc aaagcagcag agctggcaaa agtgaaacag
     5641 tggcatctgg aatactacgt tgatgaaccg accttcttcg acaaagtgat ggacaacatg
     5701 tctggttctg tccgggcaat gttgccagat gcgttccagg ccatgttacc tgcaccgctt
     5761 gcctcggtag cctctaccgt taaaagtgaa agcgacaagc tggccgcgtt taacgaccca
     5821 caaaaccgtt atgcgttttg cctgacctgc gccaacgtgc gttaagtctt gtactgagtg
     5881 gccgacagat cgtcggccac attatttttt acctcgacga atcctcttcc cgctgtctcg
     5941 acccatattt ccttatccgc atagtatcag gtgcgctcct cctgcctcac gtatatactt
     6001 ttgctctttc gatatcattc atatcaatat catgcaaaag aaatcaattt acgtcgccta
     6061 cacgggcggg accatcggga tgcagcgttc cgagcagggt tatattccgg tgtcaggtca
     6121 tctacaacgc caactggcgc tgatgccgga attccatcgc ccggagatgc cagatttcac
     6181 cattcatgaa tatacgccgt tgatggattc ttctgatatg acgccggaag actggcagca
     6241 tattgctgaa gatattaaag cgcactatga cgactatgat ggttttgtca ttctacacgg
     6301 taccgacacg atggcgtata ccgcctctgc gctgtcgttc atgctcgaga atctcggtaa
     6361 accggtcatt gtgacagggt cacaaatccc gctggctgag ttacgctctg acggacaaat
     6421 taatctgctg aatgcgttgt acgttgcggc gaattatccg atcaacgaag taacgctctt
     6481 tttcaataac cgattgtatc gcggcaaccg cactaccaaa gcccatgccg atggttttga
     6541 tgcgtttgcc tctccaaacc ttcctccgtt actggaagct ggtatccata ttcgtcgttt
     6601 gaatacgcca cccgccccgc acggtgaagg ggaattaatc gttcatccaa tcacgccaca
     6661 accaattggc gtagtgacga tttatccggg gatttctgct gatgtcgtgc gcaattttct
     6721 gcgccaaccg gtgaaagcat tgattctgcg ctcctatggc gtgggtaatg cgccacaaaa
     6781 caaagccttc ctgcaggaat tacaagaagc cagcgatcgc ggtattgtgg tggtcaacct
     6841 gacacaatgt atgtccggta aagtgaatat gggtggttat gccaccggta acgccctcgc
     6901 ccatgccggc gttattggcg gtgcagatat gactgtagaa gccacgctaa ccaaactgca
     6961 ttacctgctg agccaggaac tggatactga aaccattcgc aaggccatga gccaaaacct
     7021 gcgcggcgaa ctgacgccgg atgattaagg agactgcaat gccccctcgc gccctgttac
     7081 tggtcgattt acaaaatgat ttctgtgctg gtggcgcgct cgccgtgccg gaaggtgaca
     7141 gtacggtgga tgtcgctaac cgccttattg actggtgcca gtcgcgcggt gaagcggtta
     7201 tcgccagtca ggactggcac ccggcgaatc acggcagttt tgccagtcag cactgtgtag
     7261 agccttatat gccaggtcaa ctcgacggtt tgccacaaac cttctggcca gatcactgtg
     7321 tgcagaacag tgaaggcgca caattacatc cgttactgaa ccaaaaagcg atcgcagcgg
     7381 tgttccataa aggcgaaaat cctttagttg acagttacag tgcctttttt gataacggcc
     7441 gtcggcagaa aacctctctc gatgactggt tacgcgatca tgaaatcgat gaattgatcg
     7501 ttatgggcct ggctactgac tattgcgtga agtttaccgt gctggacgcg ttacagttag
     7561 gttataaggt aaacgtgatt accgatggtt gtcgtggcgt gaatatccag ccccaggaca
     7621 gtgcgcacgc gtttatggag atgtcagcag ctggggcaac gctatatacg ctggcagact
     7681 gggaagagac gcaggggtaa tattagttga attcatctct ggcctacatt tcctcaatga
     7741 cattgtaggc cagataatac ttgtcagtgt tgcatttaac ctacttgcag ctaaaacacg
     7801 acgtcctaac aagtatattt gccctccctt aataacaggc ttaaatacta gataactcat
     7861 tcacttaaac atagcaatag gctttgctga gataccaaaa tcttctttta actgctgttt
     7921 gctcttcatg accatctgac catttgtgtc aattgtcata tgctgtgctt cggtattatg
     7981 gcgggcttgc cacagcatca ccagttgcag gcaattctct ttttgttcct cggtcaacgc
     8041 aacgccatca ggccatttcc ccagttcaac ggcggtcgac aaacgctggt atacctcagg
     8101 cgtcatgctg ttgataatgt catcaagatt cataattttt ccgctcctgt ggaataagtt
     8161 gctgaatcgt tttttcaacc gttgatttct tcaccgtttt cgccatcagt gaagcgtaaa
     8221 gaagcagagt taacacaata acgttcgcct gttggttgcg gcccgtcggg aaagacatgc
     8281 cccagatggg catcacagtt accgcaacga atttctatgc gctgcattcc atgtgacaag
     8341 tctttgatat aacgaatgga ttcttcactc accggttcgt agaaactggg ccagccacag
     8401 ccggaatcat acttcgtttc ggaatgaaac agcggggcat cgcagatcaa acagtgatat
     8461 acgccgtcac gcttgttatg cagtaaacga cccgtaaatg gcggttctgt cccatgattc
     8521 tgcgtcacgt aaaactgcat ctcggacaaa ttttttttca gttcttctgc cgaaggttta
     8581 ttagccattt gctcacatct cactttaatc gtgctcacat tacgtgactg attctaacaa
     8641 aacattaaca ccaactggca aaattttgtc ctaaacttga tctcgacgaa atggctgcac
     8701 ctaaatcgtg atgaaaatca catttttatc gtaattgccc tttaaaattc ggggcgccga
     8761 ccccatgtgg tctcaagccc aaaggaagag tgaggcgagt cagtcgcgta atgcttaggc
     8821 acaggattga tttgtcgcaa tgattgacac gattccgctt gacgctgcgt aaggtttttg
     8881 taattttaca ggcaaccttt tattcactaa caaatagctg gtggaatata tgactatcaa
     8941 agtaggtatc aacggttttg gccgtatcgg tcgcattgtt ttccgtgctg ctcagaaacg
     9001 ttctgacatc gagatcgttg caatcaacga cctgttagac gctgattaca tggcatacat
     9061 gctgaaatat gactccactc acggccgttt cgacggtacc gttgaagtga aagacggtca
     9121 tctgatcgtt aacggtaaaa aaatccgtgt taccgctgaa cgtgatccgg ctaacctgaa
     9181 atgggacgaa gttggtgttg acgttgtcgc tgaagcaact ggtctgttcc tgactgacga
     9241 aactgctcgt aaacacatca ccgctggtgc gaagaaagtg gttatgactg gtccgtctaa
     9301 agacaacact ccgatgttcg ttaaaggcgc taacttcgac aaatatgctg gccaggacat
     9361 cgtttccaac gcttcctgca ccaccaactg cttggctccg ctggctaaag ttatcaacga
     9421 taacttcggc atcatcgaag gtctgatgac caccgttcac gctactaccg ctactcagaa
     9481 aaccgttgat ggcccgtctc acaaagactg gcgcggcggc cgcggcgctt cccagaacat
     9541 catcccatcc tctaccggtg ctgctaaagc tgtaggtaaa gtactgccag aactgaatgg
     9601 caaactgact ggtatggcgt tccgcgttcc gaccccgaac gtatccgtag ttgacctgac
     9661 cgttcgtctg gaaaaagctg caacttacga gcagatcaaa gctgccgtta aagctgctgc
     9721 tgaaggcgaa atgaaaggcg ttctgggcta caccgaagat gacgtagtat ctaccgattt
     9781 caacggcgaa gtttgcactt ccgtgttcga tgctaaagct ggtatcgctc tgaacgacaa
     9841 cttcgtgaaa ctggtatcct ggtacgacaa cgaaaccggt tactccaaca aagttctgga
     9901 cctgatcgct cacatctcca aataagttga gatgacactg tgatctaaaa agagcgactt
     9961 cggtcgctct tttttttacc tgataaaatg aagttaaagg actgcgtcat gattaagaaa
    10021 atttttgccc ttccggtcat cgaacaaatc tcccctgtcc tctcccgtcg taaactggat
    10081 gaactggacc tcattgtggt cgatcatccc caggtaaaag cctcttttgc attacagggc
    10141 gcacaccttc tctcgtggaa acctgcgggt gaagaagaag ttctgtggtt gagcaacaac
    10201 acaccgttca aaaatggcgt cgctattcgc ggtggcgtac cggtttgctg gccgtggttt
    10261 ggtccggcgg cacaacaagg tctgcctgcg cacggttttg cccgcaacct gccgtggacg
    10321 ctgaaatcac atcgtgaaga tgctgatggc gtagcgctga cttttgaatt gacgcaaagc
    10381 gaagagacga aaaaattctg gccgcacgac tttacgctgt tagcgcattt ccgcgtgggt
    10441 aaaacctgtg aaatcgatct tgaatcacat ggcgaatttg aaaccacctc tgccctgcat
    10501 acctacttta acgtgggtga tatcgctaag gtaagcgtca gtgggctggg cgatcgcttc
    10561 attgataaag taaatgacgc gaaagaagat gtactgaccg acggtattca gaccttccct
    10621 gaccgtaccg atcgcgtgta tctgaatccg caagattgca gcgtgattaa tgatgaagcg
    10681 ctgaatcgta ttatcgccgt aggccaccag catcatctga acgttgtcgg ctggaacccg
    10741 ggaccggcgc tttcagttag catgggcgac atgccggatg atggctacaa aacatttgtt
    10801 tgtgtagaaa cggcttacgc ttcagaaacg caaaaagtga ccaaagagaa acctgcacat
    10861 ctggcgcaat ccattcgcgt tgcgaaacgt taatttgcgt taatgttgtg tgccgggtgc
    10921 gatgcatccg gcacgtatca acatcacacc atatccagcg cagtttttcc ttttggtgcc
    10981 ggatacgctt tatccagcat ggttaattcc gctgacgaaa gttcgacctc aagcacagcc
    11041 gcattctgtt ggacatgggc aatcgtggcc gcttttggaa tcgccatcac accctgatgg
    11101 ctgattaccc acgccaacaa tacttgtgcc gcgctgatat tgtgagcatg tgcaatttcg
    11161 ttgactaccg cgttttttaa cagtccattg cgcaaccgcc cggcctgggc taatggactg
    11221 taagccatca ccggcatctg ctgttgctgg caccagggga gtagatcgta ctcaatccct
    11281 cgtgaaccga gatggtaaag cacctgatta gtggcacact gatttccccc cggcagctgc
    11341 cagagttcct gcatatcagc atgatcaagg ttagaaacgc cccagcggcg gatttttccc
    11401 tgggcgatca atttttccat cgctgccaca gtctcttcaa aagcgaaact gccagaccag
    11461 tgtaataagt agagatcgag ataatcagta ttgagacggc gtaaactggc ttcgcatgca
    11521 tttatcgctt tttgcccgcc agcattccac ggatagactt tagagacgag aaagacgtta
    11581 tcgcgcagac cggttaatgc ttccccaacc accttttcgg caccgccatc ggcatacatt
    11641 tcggcggtat caatgagggt taaaccgagc tcaatgcccg cgcgtagtgc agcaacttct
    11701 gttttgcgct gactggcatc ttcgcccata taccatgttc cctgccctta tggctggcag
    11761 tgagacatcg ccactaaatt gaatcatttt ttgttgcatt gtttcctccc aggtattgca
    11821 ccaccgtaat gcaaaacagg gcgtgacgcc ctgtttttat gcacaaaatg ccctggaaag
    11881 atgcattatc agaatttgta ggtgatcccg gtagaaatca ggccagtcca ggatttatcc
    11941 accatcgggc tgtcagtaac ttcatcagac agacgggtat agcgcgcggt accgtaaaca
    12001 ctccagtcgc cgaggaagtt gtagctggcg ctcagctcca ggtaagggct ccagctgtcg
    12061 ttcgggttat agccacgcag accgctgcga gcggactctt tgcgcgatac gccataatag
    12121 tattcgttct ggttttcgct gttccactgc acaccaatac ccggagtcac ggtcaggcca
    12181 ccgttggtgt aacgatacaa ccaggccata tcccagacaa tgccgttgct gttatccagg
    12241 gtatcgccag ccagggtggt acgcaggtaa ccgtactggg taaagtgagc ataagacaga
    12301 ccagccatca tggtgctctt acggtcatcc aggtgacgca tttggtgatc gccactgtct
    12361 ttcgctttga agtaaagcgg cgaccagtaa gcggtaattg aaagtttatc cgttgcgtca
    12421 ttccacaggt agtaaccacc acctaagcca cggaaccaga agttatcgcc ttcatagttg
    12481 attaccggta ctgggtaaac atcggtatcg taatctttat atgggtgctc aacgacacct
    12541 acgcctgcgc ccagggaaaa tttaccttca gcgtgcgcta cgcctgcaga cgttgcgata
    12601 agcactccaa gtgccagaag tttgagtttg gtcacaatta atcattcctt aaacaaatgt
    12661 ttagcgggcg acaaagttta cccgtcaata catcgaaacc caaccttttt acgttttcat
    12721 tttttaaagt aactgtttaa ttttcctgac gcggatgaca ccgcgcttac agccaaatga
    12781 atttagcgtt actggctacc ctggtcttta cattaattat gcaaaattta tggatgagtt
    12841 gttgatatgc cattgaaatt aagaaagccg tgcaggcaag ttttccattt gccatctacg
    12901 cttaattttg aaggtgtatc accgggcacg ttgttctcat cgtcgataaa atggcatgag
    12961 agttgctgtg ttttagcaag agacgtcgtt cagtttacct cttccgggag cctctactat
    13021 tcatatgaac ggctcttaac ctgtgctaaa aaacgaaagg acggcatacc atgaatatat
    13081 tcgatcacta tcgccagcga tatgaagctg ccaaggacga agagttcaca ctgcaggagt
    13141 ttcttaccac ttgtcggcaa gatcgcagtg cttatgccaa cgcggctgag cggctattga
    13201 tggctatcgg tgagcctgtc atggtcgata cagcccagga acccagactt tctcgactct
    13261 tttctaaccg ggtcattgca cgttatccgg catttgaaga gttttacggc atggaagacg
    13321 cgattgaaca gattgtctct tatctgaaac acgcggctca ggggctggaa gagaagaaac
    13381 aaatcctgta tctgctgggg cctgtgggtg ggggtaaatc atcgcttgct gagcgactga
    13441 aatcattaat gcagctcgta ccgatttatg tattgagcgc caacggtgag cgtagcccgg
    13501 tcaacgatca tccgttctgt cttttcaatc cgcaggaaga tgctcagatt ctggaaaaag
    13561 agtatggcat tcctcgccgt tatctcggca ccatcatgtc gccatgggcg gcaaaacgcc
    13621 tgcatgaatt tggtggcgat atcactaagt tccgggtagt gaaggtctgg ccgtcaattc
    13681 tgcaacaaat tgctatcgcc aaaacggaac ccggtgatga gaacaaccag gacatctccg
    13741 cgctggttgg gaaagtcgat attcgtaaac tcgaacacta cgcgcagaat gacccggacg
    13801 cctacggcta ttccggtgcg ctgtgccgcg ccaaccaagg gatcatggaa tttgtcgaga
    13861 tgtttaaagc accgataaaa gtgctgcatc cgctgttaac cgccactcag gaaggtaact
    13921 acaacgggac ggaaggtatc tccgccctgc cgttcaacgg gattattctc gcccactcga
    13981 acgaatccga atgggtcact ttccgtaata acaaaaacaa cgaagccttc ctcgaccgtg
    14041 tttacatcgt gaaggtgccg tattgcttac gcatttccga agagatcaaa atctacgaga
    14101 aattgcttaa tcacagtgaa ttgactcacg ccccctgcgc ccctggcacg ctggaaacgc
    14161 tgtcacgttt ttccattctt tcgcgcctta aagagccaga aaactccagc atttattcaa
    14221 agatgcgggt ttatgatggc gaaagtctga aagacaccga tcccaaagcc aagtcgtatc
    14281 aggaatatcg tgactacgcc ggtgtcgatg aagggatgaa cggtctgtcg acgcgttttg
    14341 cgtttaagat cctctcccgc gtgttcaact tcgatcatgt agaagtggcg gcaaacccgg
    14401 tccatctgtt ctacgtcctg gaacagcaga tcgagcgcga gcagttccca caagagcagg
    14461 cagaacgcta tctggagttc ctgaaaggtt atctgatccc gaaatatgcc gagtttatcg
    14521 gcaaagagat ccagacggcc taccttgaat cctattccga atatgggcaa aacattttcg
    14581 accgttatgt tacctacgcg gatttctgga ttcaggatca ggagtatcgc gatccggata
    14641 ccgggcagct gtttgaccgc gagtctctta acgccgagct ggagaaaatc gagaaaccgg
    14701 cggggatcag taatccaaaa gatttccgca acgagattgt taacttcgta ctgcgcgcca
    14761 gagcgaataa cagcggacgc aatccgaact ggaccagcta tgaaaaactg cgcacggtta
    14821 tcgagaagaa aatgttctcc aataccgagg agctgttgcc ggttatctcg tttaacgcca
    14881 aaacgtcaac cgacgagcag aagaaacacg acgactttgt cgaccgtatg atggaaaaag
    14941 gctacacccg taaacaggtg cgtttactgt gcgaatggta tttgcgcgta cgtaaatcgt
    15001 cttaacaacc ctggcccggt acacatgtta ccgggcctac aacgacagcg aaccgtgggc
    15061 ctgagaagcg gcaacacagg cgtagcatac agttggcaaa tgtagtacgg ggggcatatg
    15121 acctggttta ttgaccggcg tctgaacggc aaaaacaaaa gcatggtgaa tcgccagcgt
    15181 tttttacgcc gttataaagc gcaaattaaa cagtcgatct ccgaggccat taataagcgt
    15241 tcggtgactg acgtcgacag cggcgaatcc gtatccattc ccacggaaga tattagcgaa
    15301 ccgatgtttc atcaggggcg tggcggtctg cgccaccgcg tgcatccggg caatgaccat
    15361 ttcgtccaga acgaccgaat tgaacgtccc cagggtggcg gcggaggttc cggcagtggt
    15421 cagggccagg ccagccagga tggtgaaggt caggatgaat ttgtctttca gatttcgaaa
    15481 gatgagtatc ttgatttgct ctttgaagat ttggccttac cgaatctgaa acaaaaccaa
    15541 caacgccagc tgaccgaata taaaacgcat cgggcgggtt atactgctaa cggcgttccg
    15601 gccaatatca gcgttgtgcg ttccttgcag aactcactgg cgcgacgcac agccatgacg
    15661 gcaggcaagc ggcgggaact tcatgcactg gaagagaatt tggccatcat cagcaacagt
    15721 gaacctgcgc agctgctgga agaggaacgt ctgcgcaaag aaattgcaga attacgtgcc
    15781 aaaattgaac gcgtcccttt tattgacacc ttcgatttac gttacaagaa ctacgagaag
    15841 cggcccgatc cctccagcca ggcagtgatg ttttgcctga tggacgtttc cggttcaatg
    15901 gatcaatcca ctaaagatat ggctaagcgt ttttatattc tgctgtatct gttcctcagc
    15961 agaacgtata agaacgtgga agtcgtatac atccgccatc atacccaggc gaaagaagtc
    16021 gatgaacatg agtttttcta ctcgcaggaa acaggcggca ccattgtttc cagcgccctg
    16081 aaactgatgg atgaggtagt gaaagagcgt tataacccgg cacagtggaa tatttacgct
    16141 gcacaagcat cggacggcga taactgggcc gatgactctc cgctttgcca tgaaatcctg
    16201 gcgaaaaaat tattacctgt tgttcgttat tacagctata tcgaaattac ccgtcgtgca
    16261 catcagacat tgtggcgaga atatgagcat ctgcaatcta ctttcgacaa ctttgcgatg
    16321 cagcacatcc gcgaccagga tgatatttat ccggtgttcc gtgaactgtt tcataaacaa
    16381 aatgcaacag ctaaagacta aaactatcag ccaggtcatt atcgcctggc tgatttttag
    16441 cttactgtaa attatctcat ttattacata cattagctta caatcgcttt aaatatgaca
    16501 gcataacctt tacataattt agttccagaa aacaatcatt cggaaaaatg attcagtcaa
    16561 cacgtatttc catggggtta ttctttaaat atttt
//
LOCUS       DAPHDW010000096        16595 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.097, whole genome shotgun sequence.
ACCESSION   DAPHDW010000096 DAPHDW010000000
VERSION     DAPHDW010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.097"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            139..894
                     /gene="repE"
                     /locus_tag="RH057_003104"
     CDS             139..894
                     /gene="repE"
                     /locus_tag="RH057_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein RepE"
                     /protein_id="HDV3129438.1"
                     /translation="MAETAVINHKKRKNSPRIVQSNDLTEAAYSLSRDQKRMLYLFVD
                     QIRKSDGTLQEHDGICEIHVAKYAEIFGLTSAEASKDIRQALKSFAGKEVVFYRPEED
                     AGDEKGYESFPWFIKRAHSPSRGLYSVHINPYLIPFFIGLQNRFTQFRLSETKEITNP
                     YAMRLYESLCQYRKPDGSGIVSLKIDWIIERYQLPQSYQRMPDFRRRFLQVCVNEINS
                     RTPMRLSYIEKKKGRQTTHIVFSFRDITSMTTG"
     gene            1482..2648
                     /gene="sopA"
                     /locus_tag="RH057_003105"
     CDS             1482..2648
                     /gene="sopA"
                     /locus_tag="RH057_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid-partitioning protein SopA"
                     /protein_id="HDV3129439.1"
                     /translation="MKLMETLNQCINAGHEMTKAIAIAQFNDDSPEARKITRRWRIGE
                     AADLVGVSSQAIRDAEKAGRLPHPDMEIRGRVEQRVGYTIEQINHMRDVFGTRLRRAE
                     DVFPPVIGVAAHKGGVYKTSVSVHLAQDLALKGLRVLLVEGNDPQGTASMYHGWVPDL
                     HIHAEDTLLPFYLGEKDDVTYAIKPTCWPGLDIIPSCLALHRIETELMGKFDEGKLPT
                     DPHLMLRLAIETVAHDYDVIVIDSAPNLGIGTINVVCAADVLIVPTPAELFDYTSALQ
                     FFDMLRDLLKNVDLKGFEPDVRILLTKYSNSNGSQSPWMEEQIRDAWGSMVLKNVVRE
                     TDEVGKGQIRMRTVFEQAIDQRSSTGAWRNALSIWEPVCNEIFDRLIKPRWEIR"
     gene            2648..3619
                     /locus_tag="RH057_003106"
     CDS             2648..3619
                     /locus_tag="RH057_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family plasmid partition
                     protein"
                     /protein_id="HDV3129440.1"
                     /translation="MKRAPVIPKHTLNTQPVEDTSLSTPAAPMVDSLIARVGVMARGN
                     AITLPVCGRDVKFTLEVLRGDSVEKTSRVWSGNERDQELLTEDALDDLIPSFLLTGQQ
                     TPAFGRRVSGVIEIADGSRRRKAAALTESDYRVLVGELDDEQMAALSRLGNDYRPTSA
                     YERGQRYASRLQNEFAGNISALADAENISRKIITRCINTAKLPKSVVALFSHPGELSA
                     RSGDALQKAFTDKEELLKQQASNLHEQKKAGVIFEAEEVITLLTSVLKTSSASRTSLS
                     SRHQFAPGATVLYKGDKMVLNLDRSRVPTECIEKIEAILKELEKPAP"
     gene            4271..5173
                     /locus_tag="RH057_003107"
     CDS             4271..5173
                     /locus_tag="RH057_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HDV3129441.1"
                     /translation="MHFSGEPAQIAEIKRLASGAVTPLYRRATDEGIQLFLAGSAGLL
                     QTTEDVRFEPCPGLTAAGRGVVSPENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDEVRETITVHFTAKRGDWCGFWSNEDVSVWWNRLCDNVLPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTEQYGVKWPVGYEVNISSQGDNFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCNFCGWQRYERGELVDVLWGELEWSSP
                     TDDDELPEVTGPAWIVDKVAHYGG"
     gene            5170..5481
                     /locus_tag="RH057_003108"
     CDS             5170..5481
                     /locus_tag="RH057_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129442.1"
                     /translation="MNSDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLAPVTVYASPSPFSDERPSSRFRGIALPSKRRRLRYSTVGLTRYRT
                     R"
     gene            5557..6240
                     /locus_tag="RH057_003109"
     CDS             5557..6240
                     /locus_tag="RH057_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HDV3129443.1"
                     /translation="MSRFILGDCVRVMATFPDNAVDFILTDPPYLVGFRDRSGRTIAG
                     DKTDEWLQPACIEMFRVLKKDALMVSFYGWNRVDRFMAAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPPNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            6241..6462
                     /locus_tag="RH057_003110"
     CDS             6241..6462
                     /locus_tag="RH057_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129444.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMEQRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDACFKD"
     gene            6476..6910
                     /locus_tag="RH057_003111"
     CDS             6476..6910
                     /locus_tag="RH057_003111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
                     /protein_id="HDV3129445.1"
                     /translation="MYGTCETLCRELAVKYPGDMPLMLVIWSPEEIQALADGMDISLS
                     DHEIRTVLARLEDIPEDQRTESGISSGVAMEIINNVSENRQVTVPAELLASLIQTAEQ
                     ALWKREWAARDHGLAVPECVTRRQAVINQARTLLKNNTREND"
     gene            6955..7725
                     /locus_tag="RH057_003112"
     CDS             6955..7725
                     /locus_tag="RH057_003112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129446.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQRNPNHPGWISIYVR
                     LDAPRLATLLINRHGGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLAEDEIRAVLAAVRDAIRSICYQVADDARRIRAALTTTGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAIVNRGARFSSVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVRKARAEIRSMADALAKIRK"
     gene            8265..8567
                     /locus_tag="RH057_003113"
     CDS             8265..8567
                     /locus_tag="RH057_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV3129447.1"
                     /translation="MMPEYQGGFWHFIRLPDGGGYMMPDGDRFHMVNGANWFDRTVSA
                     DAAGIILTSLVINRQLWLYHDSGDAGLTQLYRMRDAQLWRHIEFHLECNAIYAALD"
     gene            8614..8928
                     /locus_tag="RH057_003114"
                     /pseudo
     CDS             8614..8928
                     /locus_tag="RH057_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052650.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 family protein"
     gene            8928..12644
                     /gene="traI"
                     /locus_tag="RH057_003115"
                     /pseudo
     CDS             8928..12644
                     /gene="traI"
                     /locus_tag="RH057_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001463032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            12664..13410
                     /gene="traX"
                     /locus_tag="RH057_003116"
     CDS             12664..13410
                     /gene="traX"
                     /locus_tag="RH057_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HDV3129448.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSLLVWSPGQRDIIKTVALVLM
                     VLDHANRILHLDQSWMFLVGRGAFPLFALVWGLNLSRHTHIRQEAINRLWGWAVIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRTWWRSAEAMLLLAMWLPFSGTSYGIAG
                     LLMLAVSHRLYRAEDRMERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVSC
                     TGKSLPRFWYGDFFPTFYACHLTVLGVLAV"
     gene            13469..14329
                     /locus_tag="RH057_003117"
     CDS             13469..14329
                     /locus_tag="RH057_003117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3129449.1"
                     /translation="MKITDHKLPEGIALTFRVPEGNIKHPLILLCHGFCGIRNVLLPS
                     FANAFTEAGFATITFDYRGFGESEGERGRLVPAMQTEDIISVINWAEKQVCIDNQRIG
                     LWGTSLGGCHVFNAAAQDKRVKSIVSQLAFADGEVLVTGEMNELEKASFLSTLNKMAE
                     KKKNTGKEMFVGVTRVLSDNESKVFFEKVKGQYPEMDIKIPFLTVMETLQYKPAESAA
                     KVQCPVLVVIAGQDSVNPPEQGRALYDAVASGTKELYEEADACHYDIYEGAFFERVAA
                     VQTQWFKKHL"
     gene            14432..14989
                     /locus_tag="RH057_003118"
     CDS             14432..14989
                     /locus_tag="RH057_003118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000139380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HDV3129450.1"
                     /translation="MTEQKRPVLTLKRKTEGETPIRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLTLPSLDEAVNTLKPWWPGLFDGNTPRLLACGIRE
                     VLLDEVSQRNIPLSHKKLRRALKAITRSESYLGAMKAGACRYDTEGYVTEHITQEEEQ
                     YAQARLEKVRRQNRIKDELRAILAE"
     gene            15144..15347
                     /locus_tag="RH057_003119"
     CDS             15144..15347
                     /locus_tag="RH057_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129451.1"
                     /translation="MAVSSGLSEHSSIYRYQREGRHAVRQEIRGFFREAAEVWQNAAR
                     LAPCEAWRDFALKRASACRCKCR"
     gene            16090..16551
                     /locus_tag="RH057_003120"
     CDS             16090..16551
                     /locus_tag="RH057_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HDV3129452.1"
                     /translation="MKKYIPVVLFVFSCQVFSADIHGRGVRVLDGNTIDVMLSQHPVR
                     VRLVNIDAPEKKQEYGRWSEKIMKSLVAGKTVTVTYFQRDHYGRILGQVYAPDGMNVN
                     QFMVRAGDAWVYEQYNTDPVLPVLQGEVRQQKRGLWADSAPVPPWVWRSRK"
BASE COUNT         3857 a         4041 c         4821 g         3876 t
ORIGIN      
        1 ttgtgacaaa ttgcccttaa ccctgtgaca aattgccctc agaagaagct gttttttcac
       61 aaagttatcc ctgcttattg actctttttt atttagtgtg acaatctaaa aacttgtcac
      121 acttcacatg gatctgtcat ggcggaaaca gcggttatca atcacaagaa acgtaaaaat
      181 agcccgcgaa tcgtccagtc aaacgacctc actgaggcgg catatagtct ctcccgggat
      241 caaaaacgta tgctgtatct gttcgttgac cagatcagaa aatctgatgg caccctacag
      301 gaacatgacg gtatctgcga gatccatgtt gctaaatatg ctgaaatatt cggattgacc
      361 tctgcggaag ccagtaagga tatacggcag gcattgaaga gtttcgcggg gaaggaagtg
      421 gttttttatc gccctgaaga ggatgccggc gatgaaaaag gctatgaatc ttttccctgg
      481 tttatcaaac gtgcgcacag tccatccaga gggctttaca gtgtacatat caacccatat
      541 ctcattccct tctttatcgg gttacagaac cggtttacgc agtttcggct tagtgaaaca
      601 aaagaaatca ccaatccgta tgccatgcgt ttatacgaat ccctgtgtca gtatcgtaag
      661 ccggatggct caggcatcgt ctctctgaaa atcgactgga tcatagagcg ttaccagctg
      721 cctcaaagtt accagcgtat gcctgacttc cgccgccgct tcctgcaggt ctgtgttaat
      781 gagatcaaca gcagaactcc aatgcgcctc tcatacattg agaaaaagaa aggccgccag
      841 acgactcata tcgtattttc cttccgcgat atcacttcca tgacgacagg atagtctgag
      901 ggttatctgt cacagatttg agggtggttc gtcacattta ttctgaccta ctgagggtaa
      961 tttgtcacag ttttgctgtt tccttcagcc tgcatggatt ttctcatact ttttgaactg
     1021 taatttttaa ggaagccaaa tttgagggca gtttgtcaca gttgatttcc ttctctttcc
     1081 cttcgtcatg tgacctgata tcgggggtta gttcgtcatc attgatgagg gttgattatc
     1141 acagtttatt actctgaatt ggctatccgc gtgtgtacct ctacctggag tttttcccac
     1201 ggtggatatt tcttcttgcg ctgagcgtaa gagctatctg acagaacagt tcttctttgc
     1261 ttcctcgcca gttcgctcgc tatgctcggt tacacggctg cggcgagcgc tagtgataat
     1321 aagtgactga ggtatgtgct cttcttatct ccttttgtag tgttgctctt attttaaaca
     1381 actttgcggt tttttgatga ctttgcgatt ttgttgttgc tttgcagtaa attgcaagat
     1441 ttaataaaaa aacgcaaagc aatgattaaa ggatgttcag aatgaaactc atggaaacac
     1501 ttaaccagtg cataaacgct ggtcatgaaa tgacgaaggc tatcgccatt gcacagttta
     1561 atgatgacag cccggaagcg aggaaaataa cccggcgctg gagaataggt gaagcagcgg
     1621 atttagttgg ggtttcttct caggctatca gagatgccga gaaagcaggg cgactaccgc
     1681 acccggatat ggaaattcga ggacgggttg agcaacgtgt tggttataca attgaacaaa
     1741 ttaatcatat gcgtgatgtg tttggtacgc gattgcgacg tgctgaagac gtatttccac
     1801 cggtgatcgg ggttgctgcc cataaaggtg gcgtttacaa aacctcagtt tctgttcatc
     1861 ttgctcagga tctggctctg aaggggctac gtgttttgct cgtggaaggt aacgaccccc
     1921 agggaacagc ctcaatgtat cacggatggg taccagatct tcatattcat gcagaagaca
     1981 ctctcctgcc tttctatctt ggggaaaagg acgatgtcac ttatgcaata aagcccactt
     2041 gctggccggg gcttgacatt attccttcct gtctggctct gcaccgtatt gaaactgagt
     2101 taatgggcaa atttgatgaa ggtaaactgc ccaccgatcc acacctgatg ctccgactgg
     2161 ccattgaaac tgttgctcat gactatgatg tcatagttat tgacagcgcg cctaacctgg
     2221 gtatcggcac gattaatgtc gtatgtgctg ctgatgtgct gattgttccc acgcctgctg
     2281 agttgtttga ctacacctcc gcactgcagt ttttcgatat gcttcgtgat ctgctcaaga
     2341 acgttgatct taaagggttc gagcctgatg tacgtatttt gcttaccaaa tacagcaata
     2401 gtaatggctc tcagtccccg tggatggagg agcaaattcg ggatgcctgg ggaagcatgg
     2461 ttctaaaaaa tgttgtacgt gaaacggatg aagttggtaa aggtcagatc cggatgagaa
     2521 ctgtttttga acaggccatt gatcaacgct cttcaactgg tgcctggaga aatgctcttt
     2581 ctatttggga acctgtctgc aatgaaattt tcgatcgtct gattaaacca cgctgggaga
     2641 ttagataatg aagcgtgcgc ctgttattcc aaaacatacg ctcaatactc aaccggttga
     2701 agatacttcg ttatcgacac cagctgcccc gatggtggat tcgttaattg cgcgcgtagg
     2761 agtaatggct cgcggtaatg ccattacttt gcctgtatgt ggtcgggatg tgaagtttac
     2821 tcttgaagtg ctccggggtg atagtgttga gaagacctct cgggtatggt caggtaatga
     2881 acgtgaccag gagctgctta ctgaggacgc actggatgat ctcatccctt cttttctact
     2941 gactggtcaa cagacaccgg cgttcggtcg aagagtatct ggtgtcatag aaattgccga
     3001 tgggagtcgc cgtcgtaaag ctgctgcact taccgaaagt gattatcgtg ttctggttgg
     3061 cgagctggat gatgagcaga tggctgcatt atccagattg ggtaacgatt atcgcccaac
     3121 aagtgcttat gaacgtggtc agcgttatgc aagccgattg cagaatgaat ttgctggaaa
     3181 tatttctgcg ctggctgatg cggaaaatat ttcacgtaag attattaccc gctgtatcaa
     3241 cacagccaaa ttgcctaaat cagttgttgc tcttttttct caccccggtg aactatctgc
     3301 ccggtcaggt gatgcacttc aaaaagcctt tacagataaa gaggaattac ttaagcagca
     3361 ggcatctaac cttcatgagc agaaaaaagc tggggtgata tttgaagctg aagaagttat
     3421 cactctttta acttctgtgc ttaaaacgtc atctgcatca agaactagtt taagctcacg
     3481 acatcagttt gctcctggag cgacagtatt gtataagggc gataaaatgg tgcttaacct
     3541 ggacaggtct cgtgttccaa ctgagtgtat agagaaaatt gaggccattc ttaaggaact
     3601 tgaaaagcca gcaccctgat gcgaccacgt tttagtctac gtttatctgt ctttacttaa
     3661 tgtcctttgt tacaggccag aaagcataac tggcctgaat attctctctg ggcccactgt
     3721 tccacttgta tcgtcggtct gattattagt ctgggaccac ggtcccactc gtatcgtcgg
     3781 tctgattatt agtctgggac cacgatccca ctcgtgttgt cggtctgatt atcggtctgg
     3841 gaccacggtc ccacttgtat tgtcgatcag actatcagcg tgagactacg attccatcaa
     3901 tgcctgtcaa gggcaagtat tgacatgtcg tcgtaacctg tagaacggag taacctcggt
     3961 gtgcggttgt atgcctgctg tggattgctg ctgtgtcctg cttatccaca acattttgcg
     4021 cacggttatg tggacaaaat acctggttac ccaggccgtg ccggcacgtt aaccgggctg
     4081 catccgatgc aagtgtgtcg ctgtcgccgg cctcctcacc cggtcacgtt tcgtcgttcc
     4141 tcctccacgc gctgcggctt cggggccgca cctgcattcg tatgcggtcg cccggttaca
     4201 ggtgcggcac ggcctgatgg aggccgcatg tgagaggaga attcccatgc caaactggtg
     4261 ctcaaatcgt atgcattttt ctggtgaacc agcacagatc gctgagatta aacgactggc
     4321 cagcggtgca gtcacaccgc tttatcgccg cgccacagat gaaggtattc agctgtttct
     4381 ggccggtagt gccggacttc tgcagaccac tgaagatgtg cggtttgaac catgccccgg
     4441 actgacggct gccgggcgtg gtgttgtatc gccggagaat atcgcgttca cccgctggct
     4501 gacacacctg cagaacggtg tgctactgga tgaacaaaac tgcctgatgc tgcatgaact
     4561 ctggctgcag agtggtactg gccagcgtcg ctgggaagga ttaccggatg aggtcaggga
     4621 aaccatcacc gtgcatttca ccgccaaaag aggtgactgg tgtggcttct ggagtaacga
     4681 ggatgtatcg gtgtggtgga accgtctgtg tgacaatgta ctgccggaaa aaaccatgcc
     4741 gtttgacctg ctgacggttc tgcccacccg cctggatgtt gaagtgaatg gctttaacgg
     4801 tggtgttctg aacggtgttc cttctgcgta tcactggtat acggaacagt atggcgtgaa
     4861 gtggcctgtg gggtatgagg tgaatatcag cagtcaggga gacaacttca ttcaggttga
     4921 tttcgacacg ccgtggtgtc agccggaaag cgacgttatt gcagaattaa gccgccgttt
     4981 cagttgcaca ctggagcact ggtatgccga gcagggctgc aatttctgtg gctggcagcg
     5041 atatgagcgt ggagagcttg ttgatgtcct gtggggggaa ctggaatggt cttccccgac
     5101 agatgacgat gagctgccgg aagtcaccgg acctgcgtgg attgtcgaca aggtggcgca
     5161 ttacggtgga tgaattctga cggagacggg cgggcaaccg cccgtttctt ttccgacaaa
     5221 ggatgtcgcc gtgctcctct ttttactgcg ccggctgacg cggcgcggca taaacgctgc
     5281 ctgtggtcag tgtcccgcgt ccggcgggca cgggacgggc gcttttaccg ctcccggctg
     5341 gcaccggtga cagtgtacgc cagcccgtcg cccttttctg atgaacgccc ttcaagccgc
     5401 tttcgcggca tagccttgcc gtcaaaaaga cgacggctgc ggtattccac ggtcggcctg
     5461 acccgttacc ggacgcggtg aacagcccac aggccgcggg gaacgggcac cacaggggtg
     5521 ccctcccggt gccttctgta agagaaggag gcttttatgt cccgttttat cctgggtgac
     5581 tgcgtgcgag ttatggccac gtttccggac aatgctgtcg atttcatcct caccgacccg
     5641 ccgtatctcg tcggtttccg tgaccgttcc gggcgtacca ttgccggcga taaaaccgat
     5701 gaatggctgc aaccggcctg tattgaaatg tttcgcgtac tcaaaaaaga tgcgctgatg
     5761 gtcagcttct acggctggaa ccgcgtcgat cgctttatgg ccgcctggaa aaatgcggga
     5821 ttcagcgttg ttggtcacct ggtcttcacc aaaaactaca catcgaaggc cgcatatgtg
     5881 ggctatcgcc acgaatgcgc ctacatcctg gcaaaaggcc gtccacgtct gccaccaaac
     5941 ccgctgccgg acgtgctggg ctggaaatat tcgggcaatc gtcatcaccc gacggaaaag
     6001 cccgttacca gcctgcaacc gctgattgag agcttcacac acccgaacgc aattgtgctg
     6061 gacccgtttg caggcagcgg ctcaacctgc gtcgccgccc tccagtccgg acgccggtat
     6121 atcggtatcg agctgcttga gcagtatcac cgtgccgggc agcaacgcct tgccgccgtg
     6181 caacgggcca tgcagcaggg ggccgcgaat gatgactggt ttatgccgga ggctgcgtaa
     6241 atgaactatg ccggacacga aaaactgcgc gccgaagtgg cggaggtggc caatgccatg
     6301 tgcgacctgc gtacaaccat gaatgagatg gagcagcggt acagctttaa tgccgacacc
     6361 ctgccggaac gtctggtgcg tcagacgctg tttcgcgcaa accgcctcct gatggaggca
     6421 tataccgaaa ttctggaact ggatgcgtgc tttaaagatt gagaaggaga cgagaatgta
     6481 cggaacatgc gaaacgctat gccgggagct ggcagtaaag tatccgggag acatgccgct
     6541 gatgctggtt atctggtccc cggaagagat tcaggccctc gctgacggaa tggatatttc
     6601 cctgtccgat catgaaatca gaaccgtcct ggcgcgcctg gaggacatcc cggaagacca
     6661 gcggactgaa tccggtattt cttccggcgt ggcgatggag atcatcaata acgtgagcga
     6721 aaaccgccag gtgaccgtcc ctgctgaact gctggcgtcc ctgattcaga ccgctgaaca
     6781 ggcattgtgg aaacgtgaat gggccgcccg ggatcatggc ctcgccgtcc cggaatgcgt
     6841 cacccgccgt caggcggtga ttaatcaggc ccgcaccctg ctgaaaaaca acacacgcga
     6901 aaacgactga tgttatcgcc gcctgcgggc ggcgattcag ggagaccatg aatcatgaac
     6961 gaaacgctga atgcactgat ttgtcgccac gcccgtaacc tgctgctggc gcagggctgg
     7021 ccggaagaga cggatgttga ccagcgaaat ccgaaccatc cgggctggat cagcatttac
     7081 gtgcggctgg atgcgccccg gctggcgacg ttacttatca accgtcacgg cggcgtactg
     7141 ccgccgcacc tggcctctgc cattcagaaa ctgaccggaa ccggggcgga acttgttctg
     7201 tccggcagtc agtggcagtc gctgccggta cttccggcag acggaacgca ggtgtctttc
     7261 ccgtatgccg gtgagtggct ggcagaagac gaaatcaggg ctgtacttgc ggcggtgcgc
     7321 gatgcaatac gaagcatctg ttaccaggtg gcagacgatg cgcggcgtat ccgggcggcg
     7381 ctgaccacca ccggtcagac gttgctgacc cgccagacgc gccgctttcg tctggttgtg
     7441 aaggaaagcg atcacccctg ctggctcgat gaagatgacg aaaacctgcc cgtggtgctc
     7501 gatgccatcg tgaaccgggg agcacgtttt tcgtcggtgg aaatgtacct ggtcagcgat
     7561 tgtattgagc atatcctgtc cagtgggctg gcctgcgatg tgctgcgtat accggatgaa
     7621 ccgccccgcc gctggtttga ccgtggtgtt ctgcgggagg tggtccggaa agcccgggcc
     7681 gaaatccgca gcatggcgga tgccctggca aaaatccgga aatgatgacg ttggcagggt
     7741 aaatccccgc ctttttcctg ccggtccccg gacagagcac aaaggaagtc gccgccggct
     7801 ccgctttacc cggccatgca gggcatggcc ttgtggtttt tcagttatgt ggcctcagcg
     7861 tcgtgtgcgg gctgtgccgt gcctccatct tagccgggct ggcgttgatg caagggtacg
     7921 cttcgccgct gcggtcaccc ggtccctcct tcccgtctgc cgtgattttc cggtcgttca
     7981 cccgctcaga tttcgcggtc tcaccctgca actcccgtca gccgtgtgcg tcaggctgcg
     8041 gcttcccttg cgcccctgca tccccgcctg ttcgcccggc ttttatggag gcacggcacc
     8101 gcccggtgtc gcagaatatg tgaacaacgg aggaaaacat catgcaatat gcgaaacctg
     8161 tcactctgaa tgttgaagag tgcgaccgtc tctcctttct gccgtatctg tttggcaacg
     8221 attttctgta tgccgaggcg tatgtgtacg cgctggcgca aaaaatgatg ccggaatatc
     8281 agggaggctt ctggcacttc atccgcctgc cggacggtgg cggttacatg atgccggatg
     8341 gcgaccgttt ccacatggtg aacggtgcaa actggtttga ccgtaccgtg agtgctgatg
     8401 ccgcaggcat catcctgacc tcccttgtga ttaaccgcca gttgtggctg taccacgaca
     8461 gcggggatgc aggactgacc cagctttacc ggatgcgcga tgcgcagttg tggcgtcaca
     8521 tcgaatttca cctcgaatgc aacgcgattt acgcggctct ggactgatta acggacgggg
     8581 cggcaatgcc gcccctgtaa aggagacgag aacatgtact gtactgttaa agaaattatc
     8641 cgcgaagtgc tggatacaga cgtgccggac agtgaatgcg tttttgccgt agtgctgacc
     8701 cgtggggatg tgcgtcacat agcccaggac tggagtctga cagacgatga gctggaaacc
     8761 gtcatgcagc ggctggacga tgcctttgaa tatggtgcgg atgtcagcgt tgttcacggc
     8821 gttgttcgtg aactgatgga agaaaagcgc gccagccgtc aggtgacagt cccggcggtg
     8881 atgctggaaa aagtgatggc gctggcaggc agtgaaatga agcgcctgta accctgctgg
     8941 acggtgccgc acgtcataac gtacaggtcc tgataaccga cagcgggcag cgaaccggta
     9001 caggcagtgc actgatggcc atgaaagatg ccggggtgaa cacatatcgc tggcaggggg
     9061 gagaacagcg gccggccacc atcatcagtg aaccggaccg taatgtccgc tatgcccggc
     9121 tggcaggaga ttttgctgcc agcgtgaaag ccggagaaga gagcgtggca caggtcagcg
     9181 gggtacggga acaggccata ctgacacagg ccattcgcag tgagctgaaa acacagggcg
     9241 tgctcggacg cccggaggta accatgaccg ccctgtcgcc ggtctggctg gacagccgga
     9301 gccgttatct gcgggatatg taccgtccgg ggatggtgat ggagcagtgg aacccggaga
     9361 cacgcagtca tgaccgctat gttatcgacc gggtgacggc gcagagtaac agcctgaccc
     9421 tgcgggatgc gcagggtgaa acgcaggtgg tgcgtatttc ctccctggac agcagctggt
     9481 cgctgttccg gccggaaaaa atgccagtgg cagacggcga gcgactgagg gtgacaggga
     9541 aaattcccgg actccgcgtc tccggcggtg accgcctgca ggtggcatcc gtcagtgaag
     9601 atgcgatgac ggttgttgtg ccggggcggg ctgaaccggc caccctgcct gtgagcgatt
     9661 caccattcat ggcactgaag ctggagaacg gctgggtgga aacgcctggg cattccgtca
     9721 gcgacagtgc gaaggttttt gcctccgtca cacagatggc aatggacaac gccaccctga
     9781 acggtctggc ccgcagcggt cgtgatgtcc ggctgtattc ctcactggat gaaacccgta
     9841 ctgcggaaaa acttgcccgc catccctcct ttacggtggt ttctgagcag ataaaggcgc
     9901 gtgccggtga gacattgctg gaaaccgcta tcagtctgca gaaaaccggg cttcacacgc
     9961 cggcacagca ggctattcat ctggcgcttc ctgtggtgga aagtaaaaac ctggccttca
    10021 gcatggtgga cctgctgaca gaggcgaagt cgtttgctgc agaaggaacc agttttactg
    10081 aactgggagg ggaaatcaat gcgcagataa aacgcggtga tttactgtat gtggatgtgg
    10141 caaaaggcta tggcacaggc ctgctggttt cccgtgcgtc gtatgaggca gaaaagagca
    10201 ttcttcgcca tattctcgaa ggtaaggagg cggtcacgcc actgatggag agagtacctg
    10261 gcgaactcat ggagaaactg acatcaggac agcgtgccgc tacccgcatg atactggaaa
    10321 cgtccgaccg tttcacggtg gtgcagggct atgccggtgt gggtaagacc acacagttcc
    10381 gggcggtgat gtcagccgtg aacatgctgc cggagagtga acgtccccgt gtcgtggggc
    10441 tggggcccac gcaccgtgcg gtcggtgaga tgcgcagcgc cggcgtggat gcacagacgc
    10501 tggcgtcctt tctgcatgac acgcagctgc tgcagcgcag cggagaaacg ccgaatttca
    10561 gcaacacgct gttcctgctc gatgagagct ctatggtggg caataccgac atggcacggg
    10621 catacgccct gattgcggcc ggtggcggtc gtgccgtggc cagtggtgac acggaccagt
    10681 tgcaggccat cgcgcccggt cagcctttcc gtctccagca gacgcgcagt gctgccgatg
    10741 tggccatcat gaaggagatt gtgcgccaga cgccggagct gcgggaggcg gtatacagcc
    10801 tgattaaccg ggatgtggaa agggcactgt ccgggcttga gagtgtgaaa ccgtctcagg
    10861 tgccacgtca ggagggcgca tgggtaccgg agcactccgt gacggagttc agtcacagcc
    10921 aggaagcgaa actggcagaa gcgcagcaga aggcgatgct gaaaggtgag gcttttccgg
    10981 atattcccat gacactgtat gaagccattg tccgcgatta taccggcagg acgccggaag
    11041 cacgggagca gacgctgatt gtcacgcatc tgaatgagga ccggcgcgta ctgaacagca
    11101 tgattcatga tgcacgggaa aaggccggtg agctgggaaa agagcaggtc atggcgcctg
    11161 tcctgaacac agcgaatata cgcgacgggg agttgcgtcg tctctccacc tgggagacac
    11221 atcgggacgc acttgccctg gtggataatg tgtatcaccg gattgccggt atcagcaagg
    11281 atgacgggct gataaccttg caggatgcgg aaggtaacac gcggctgatt tcgccccggg
    11341 aggcggtggc tgaaggcgtc acactgtaca ctccggacac catccgggtg ggaaccggtg
    11401 accggatgcg cttcacgaag agtgaccggg agcgcggtta tgtggccaac agcgtctgga
    11461 cggtgacagc ggtttccggt gacagtgtca cgctgtcgga cggacagcag acccgggtga
    11521 ttcgccccgg tcaggagcgg gcagagcaac atattgacct ggcctatgcc atcaccgccc
    11581 acggtgcgca gggggcaagt gaaacctttg ccattgcgct tgaaggcacg gaaggtaacc
    11641 ggaaacagat ggccggtttt gagtcagcct acgtggccct gtcgcgtatg aagcagcatg
    11701 tgcaggtgta taccgataac cgtcagggct ggacggatgc cattaacaat gccgtacaga
    11761 aaggaactgc ccacgatgtg ctagagccga aatcggaccg ggaggtcatg aatgcagagc
    11821 ggctgttcag tacggcgcgg gagctgcggg acgtggtggc cggccgtgcc gttctccgtc
    11881 aggcagggct ggccggggga gacagtcctg cacggtttat tgcacccggg cgcaaatatc
    11941 cgcagccgta tgtggcactg ccggcttttg accgtaacgg caagtcagct ggtatctggc
    12001 tgaacccgct gaccacggat gacggaaacg ggctgcgggg cttcagtggt gaaggccggg
    12061 tgaaaggcag cggggatgcg cagttcgtgg ccctgcaggg cagccgtaac ggagagagcc
    12121 tgctggctga taatatgcag gatggtgtcc agattgcccg tgataatcct gacagcggtg
    12181 tggtggtaag aatcgccggt gaaggtcgtc cgtggaatcc cggtaccatc accggtggtc
    12241 gcgtgtgggg ggatatcccg gacaacagcg ttcagccggg agccggaaat ggcgaaccgg
    12301 tcacggcaga ggtactggca cagcggcagg ctgaagaggc catccgccgt gaaacggaac
    12361 gccgtgcgga tgaaattgtc cgtaaaatgg cagagaacaa acctgacctg ccggacggca
    12421 aaacagagca ggctgtcagg gatattgccg ggctggagcg tgacaggtct gccatatctg
    12481 aacgggaagc tgcgctgccg gagagtgtac tgcgtgaacc acaacgggtg cgggaagcgg
    12541 tccgggaggt tgcccgggaa aatctgctgc aggagcgact gcagcagatg gagcgggata
    12601 tggttcgtga cctgcagaaa gagaaaaccc tgggcggaga ctgacacagg aagataaacg
    12661 ctgatgacaa cggataacac gaacacaaca cgtaatgatt cactggctgc ccggaccgat
    12721 acctggttgc agtcgttgct ggtctggtca cccggacagc gggatatcat caaaacggtg
    12781 gcactggtgc tgatggttct ggaccatgca aaccgcattc tgcatctgga ccagtcgtgg
    12841 atgtttctgg tcgggcgtgg ggcctttccg ctgtttgccc tggtgtgggg gctgaatctg
    12901 tcccgtcata cgcatatccg gcaggaggcc attaaccggc tgtggggatg ggcggtgatt
    12961 gcccagttcg catattatct ggccggcttt ccctggtatg aggggaatat cctgtttgcc
    13021 tttgcagttg cggcacaggt gctgacatgg tgtgaaacgc gcacctggtg gcgttccgca
    13081 gaggccatgt tgcttctggc aatgtggttg cctttctccg gcaccagtta cggcattgcc
    13141 gggctgctga tgctggcagt cagccacagg ctgtaccggg cggaagacag aatggaacgt
    13201 ctggcgctgg tcgcctgcct gctggctgtt attccggcgc ttaaccttgc caccagtgat
    13261 gcagcggcgg tggccggcct tgtgatgacg gtgctgactg ttggtctggt gtcctgtaca
    13321 gggaaatcac taccccgttt ctggtacggg gattttttcc cgacgttcta tgcctgtcat
    13381 cttactgtgc tgggcgttct ggctgtgtga ctggtatgat gctggcgctg attctgctta
    13441 ttaataaaca acataacaca ggatatttat gaaaattacg gatcacaaac tccctgaagg
    13501 tattgccctg acatttcgtg tcccggaagg aaatataaaa catcctttga ttctcttatg
    13561 ccatggattt tgcggtatcc ggaatgttct gctgccttcc tttgcgaatg cgtttacaga
    13621 agccgggttt gcaacgatta cttttgatta tcgtggattc ggtgagagtg aaggggaacg
    13681 aggtcgtctg gttccggcaa tgcagaccga agatattatt tcagttatca actgggcaga
    13741 aaagcaggta tgtattgata atcaacgtat aggattatgg ggaacatcgc tgggtgggtg
    13801 tcatgtattc aatgcagctg cgcaggataa aagagtgaaa agtattgtca gccagctggc
    13861 ttttgctgac ggtgaggttc tggttaccgg agaaatgaat gaactggaga aagcttcttt
    13921 tctgtccacg ctgaataaaa tggcagaaaa gaagaaaaat accgggaaag agatgtttgt
    13981 gggggttacc cgggtactga gtgataatga atcaaaggta ttcttcgaga aggtaaaagg
    14041 tcagtatcct gaaatggata taaaaatacc tttccttacg gttatggaga cgttgcaata
    14101 taaaccggca gagagcgcag ccaaagtaca gtgcccagtt ttggttgtta ttgccggcca
    14161 ggatagtgtt aatccaccag aacagggaag agccttatat gatgctgttg cttctggtac
    14221 taaggaatta tacgaggaag ctgacgcatg ccattatgat atttatgaag gcgcgttttt
    14281 tgaacgtgtg gcggcagtac agacgcaatg gtttaaaaaa catctttaat tgtcggtatt
    14341 aatatgtcag aggtcgttga ggtcagctta atatcatagt gagtagtttc aacgacctgg
    14401 aggacatatt ttcacaaagg tcagggcaat catgacagag cagaaacgac cggtactgac
    14461 actgaaacgg aaaacggaag gggaaacacc gatccggagc cggaaaacca tcatcaatgt
    14521 caccacgcca ccaaaatgga aggtgaaaaa gcagaaactg gcggagaagg ctgcccggga
    14581 agcagagctg gcggcgaaga aagcacaggc cagacaggcg ctgtccattt atctgacgct
    14641 gccctcgctg gatgaggcgg tgaacactct gaaaccctgg tggccgggat tgtttgacgg
    14701 taacacgccc cggcttcttg cctgtggtat ccgggaagtt ttactggatg aggtgtctca
    14761 gcggaatatc ccgctctctc ataaaaaact gcgcagggca ctgaaggcca tcacccgttc
    14821 agaaagctat cttggcgcca tgaaagccgg tgcctgccgc tatgatacag aaggctatgt
    14881 gacagaacat atcacacagg aagaagaaca gtatgcgcag gcgcgcctgg aaaaggtgcg
    14941 cagacagaac cggatcaagg atgagctgcg ggcgatcctg gcagaataat aaaagcgccc
    15001 tctgtatgac agagggcaac agcgaaaaaa atcgggaaaa cagacatcca gagttttgat
    15061 ggagaaaaac atccaaccgt tcagggatgt ttcataatgt ttctgacata atccgggtgt
    15121 cgggtcaaga ggagggataa aaaatggctg tatccagtgg attatctgaa cacagcagta
    15181 tttatcgcta tcagcgcgag gggcgtcatg ccgtccgtca ggagattcgt ggctttttca
    15241 gggaagcggc agaggtctgg cagaatgcgg cccggctggc accctgtgaa gcatggcggg
    15301 attttgctct gaagagggca tctgcatgtc ggtgtaagtg ccgataaaaa taacccccgt
    15361 cgcggagctc cgggggatca gacttatatg gcagatcgtt attattatta tagtggtgta
    15421 agaaggagtt gccctgtctt tcaaacggac atcacagaat taacatattt cacatcccgg
    15481 gtaagccagc agggataaaa aatgtgatga atttcaagaa gcaggaggtg acagtggcat
    15541 gcataacagt agggttatcc ggtgaatctg acagacagcc gggctgtatt cgcggcagga
    15601 taaagaaaaa ccgggagatg actctcccgg tccgtcttat cgctaatccc ctgagcaggg
    15661 catgtatgac ttatctatga ccgttatatt cagcgccaca tccggtagcc tgcgatataa
    15721 cacagttatc ctgtcagcaa gagcaggtat gatgaataca cggagattaa aaatataatc
    15781 tccgtcacat gttacccacg gaaaccggta acgccttcac gggaaatatg tgacagaaaa
    15841 agcagcctcc gttactccag gtatcaggag gctgaataat gcatgagacg ttgtttctta
    15901 tgtgtctggt caggctggct gaccagcgtt tttagtaaca catagccata tgtgtattaa
    15961 gtcacctcat cttattttgt tgaattgatc acaactcaac ggtttatttt ttgcgtgata
    16021 cgtgagccat tattcatccc ggcatggaat tcatgcaatc acgaaaagta atcagtgacg
    16081 gagagtaaaa tgaagaaata tataccagtg gtattatttg ttttttcatg tcaggtattt
    16141 tctgctgata ttcatggacg gggcgtgcgt gttctggatg gcaataccat tgacgtcatg
    16201 ttatctcagc atccggtgcg tgtccgtctt gttaatattg atgcacctga aaagaaacag
    16261 gaatatggac ggtggtctga aaaaataatg aaatctctgg ttgcgggaaa aacggttact
    16321 gtcacatatt ttcagcgaga tcattatggt cgtattctcg ggcaggttta tgcaccagac
    16381 ggaatgaatg ttaatcagtt tatggtccgt gcaggcgatg catgggttta tgagcagtac
    16441 aacaccgatc ctgttcttcc tgtcctgcag ggagaagtac gacagcagaa acgtggtctg
    16501 tgggcagaca gcgctcctgt tccgccctgg gtatggcgct cacggaaata atgatatttc
    16561 tgtggctatt gagttctcat tgaaatgcga tattt
//
LOCUS       DAPHDW010000097        16589 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.098, whole genome shotgun sequence.
ACCESSION   DAPHDW010000097 DAPHDW010000000
VERSION     DAPHDW010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16589
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.098"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            171..323
                     /gene="mokC"
                     /locus_tag="RH057_003121"
     CDS             171..323
                     /gene="mokC"
                     /locus_tag="RH057_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HDV3129453.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(427..1557)
                     /gene="dnaJ"
                     /locus_tag="RH057_003122"
     CDS             complement(427..1557)
                     /gene="dnaJ"
                     /locus_tag="RH057_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HDV3129454.1"
                     /translation="MAKQDYYEILGVSKTAEEREIKKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(1646..3562)
                     /gene="dnaK"
                     /locus_tag="RH057_003123"
     CDS             complement(1646..3562)
                     /gene="dnaK"
                     /locus_tag="RH057_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HDV3129455.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTTPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            3939..4343
                     /locus_tag="RH057_003124"
     CDS             3939..4343
                     /locus_tag="RH057_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HDV3129456.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKILGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKERQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            4369..5082
                     /gene="msyB"
                     /locus_tag="RH057_003125"
     CDS             4369..5082
                     /gene="msyB"
                     /locus_tag="RH057_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV3129457.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSVLM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKNMDEEHKQEFLHAIDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSAYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            5231..5797
                     /gene="satP"
                     /locus_tag="RH057_003126"
     CDS             5231..5797
                     /gene="satP"
                     /locus_tag="RH057_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HDV3129458.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(5832..6419)
                     /gene="mog"
                     /locus_tag="RH057_003127"
     CDS             complement(5832..6419)
                     /gene="mog"
                     /locus_tag="RH057_003127"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HDV3129459.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAAGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(6534..7487)
                     /gene="tal"
                     /locus_tag="RH057_003128"
     CDS             complement(6534..7487)
                     /gene="tal"
                     /locus_tag="RH057_003128"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HDV3129460.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPTLLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            7766..9196
                     /locus_tag="RH057_003129"
     CDS             7766..9196
                     /locus_tag="RH057_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HDV3129461.1"
                     /translation="MPDFFSFINSVLWGSVMIYLLFGAGCWFTFRTGFVQFRYIRQFG
                     KSLKNSIHPQPGGLTSFQSLCTSLAARVGSGNLAGVALAITAGGPGAVFWMWVAAFIG
                     MATSFAECSLAQLYKERDVNGQFRGGPAWYMARGLGMRWMGVLFAVFLLIAYGIIFSG
                     VQANAVARALSFSFDFPPLVTGIILAVFALLAITRGLHGVARLMQGFVPLMAIIWVLT
                     SMVICVINIGQLPHVIWSIFESAFGWQEAAGGAAGYTLSQAITNGFQRSMFSNEAGMG
                     STPNAAAAAASWPPHPAAQGIVQMIGIFIDTLVICTASAMLILLAGNDTTYMPLEGIQ
                     LIQKAMRVLMGSWGAEFVTLVVILFAFSSIVANYIYAENNLFFLRLNNPKAIWCLRIC
                     TFATVIGGTLLSLPLMWQLADIIMACMAITNLTAILLLSPVVHTIASDYLRQRKLGVR
                     PVFDPLRYPDIGRQLSPDAWDDVSQE"
     gene            9266..10042
                     /gene="yaaA"
                     /locus_tag="RH057_003130"
     CDS             9266..10042
                     /gene="yaaA"
                     /locus_tag="RH057_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HDV3129462.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPENARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(10195..10491)
                     /locus_tag="RH057_003131"
     CDS             complement(10195..10491)
                     /locus_tag="RH057_003131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HDV3129463.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(10705..11991)
                     /gene="thrC"
                     /locus_tag="RH057_003132"
     CDS             complement(10705..11991)
                     /gene="thrC"
                     /locus_tag="RH057_003132"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HDV3129464.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIAGDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSLGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFAALRKLMMNHQ"
     gene            complement(11992..12924)
                     /gene="thrB"
                     /locus_tag="RH057_003133"
     CDS             complement(11992..12924)
                     /gene="thrB"
                     /locus_tag="RH057_003133"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HDV3129465.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVTPVDGALLGDVVTVEAAETF
                     GLNNLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(12926..15388)
                     /gene="thrA"
                     /locus_tag="RH057_003134"
     CDS             complement(12926..15388)
                     /gene="thrA"
                     /locus_tag="RH057_003134"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HDV3129466.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTNVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHLLRHAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELMKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(15414..15533)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(15469..15534)
                     /gene="thrL"
                     /locus_tag="RH057_003135"
     CDS             complement(15469..15534)
                     /gene="thrL"
                     /locus_tag="RH057_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HDV3129467.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(15748..16434)
                     /locus_tag="RH057_003136"
     CDS             complement(15748..16434)
                     /locus_tag="RH057_003136"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HDV3129468.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLVESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
BASE COUNT         3842 a         4410 c         4122 g         4215 t
ORIGIN      
        1 gagagcgata taatagcggc gggtgcttga ggctatctgt ctcaggcatt agctgaacgg
       61 cagatagaga aaagccccga gtgatatttt actatcaacc cgaggccccc tatatgctga
      121 acacatgtag agtgcctctt actgaccgta aggtcaagga gaagagagca atgaagcagc
      181 ataaggcgat gattgtcgcc ctgatcgtca tctgtatcac cgccgtagta gcggcgctgg
      241 taacgagaaa agacctctgt gaggttcaca tccgaactgg ccagacggag gttgctgttt
      301 tcacggctta cgaatccgag taagagcaac ggcggggagt gatccccgcc actttacagg
      361 tgcttgcata tcttcaacgc accctggttt tacccaggcc tgcccacggg caggcttttg
      421 gggaggttag cgggtcaggt cgtcaaaaaa cttcttcaca ccatcaaaga agctctttga
      481 gcgcgggctg ttgtgctcac cggttgggcc accgaagctt tcttgcagct cttgcagcaa
      541 ctgtttctgc ttctcgttca ggcctaccgg tgtttcgacg acaacgcggc acagcaaatc
      601 accctgtgcg ccaccgcgga cagacttgac gcctttaccg cgcatacgga aaagcttacc
      661 ggtctgggtt tcgccaggca ctttcagttt gacgcgacca tcaagggtcg gtacttcgat
      721 ttcgccaccc agcgccgcca tagcgaagtt gatcgggact tcgcaataga ggttgttgcc
      781 ttcacgctcg aaaatcgggt gctgtttaac ctgaacctga acgtacagat cgcctgccgg
      841 tgcgccatgc tcgcccgctt caccttcgcc cgcaagacgg atgcggtctc cagtgtccac
      901 ccctgccggg attttaacgg acagcgtttt gctgcgctca acacgaccat gaccatgaca
      961 tttgttgcac ggatctttga tcagcgtacc gcggccctga cagtgtggac aggtctgctg
     1021 tacagcgaag aatccctgac gcatctgcac ctgaccagaa ccatgacagg tcggacaggt
     1081 ctgcggctgt gtacctggtt ttgcaccgct accgtggcaa acgtcacact cttccagagt
     1141 cggaatgcgg atctctttgg tcacgccacg tacagcttct tcgagggtga gctccatgtt
     1201 atagcgtaaa tcagcaccgc gcgccgcacg ttgacgacca cgtccgccgc caaaaatatc
     1261 gccgaaaacg tcaccaaaaa tatcgctgaa gtctgcgccg ccgccaaaac cgccgccgcc
     1321 cataccacct tgctcaaacg cagcatgacc atactgatcg tatgccgcac gtttttgcga
     1381 gtcggtcaga acttcataag cttccttgat ctctttaaat ttcgcctcgg cctctttgtc
     1441 accctggtta cggtccgggt ggtatttcat ggccaggcgt ttgtaggcct ttttgatttc
     1501 acgctcttcc gctgttttgg aaacgcctaa aatctcgtaa taatcttgct tagccatctt
     1561 ttttaaattg cccctagatg aatgcacggg cggagaggaa attccccttc gcccgtgtca
     1621 gtataattac ccgtttatag ggcgattatt ttttgtcttt gacttcttca aattcagcgt
     1681 cgacaacatc gtcatctttc gcgttgtttg cagaagcatc agcaccggca gtctgctgct
     1741 gggcatgttg ctgctgggcg atttccatca gtttctggga aacctgtgcc agctcctgca
     1801 ttttcgcttc gatagcggct ttgtcttcac ctttcagagc agtttccagt gcagtcagtg
     1861 cagactcgat agcagttttg tcgtcagccg gcagtttgtc gcctgcttct tcaacctgct
     1921 tacgggtgct gtgcagcaga tggtcgccct ggttgcgagt ctgtaccagc tcttcaaact
     1981 tacggtcagc ttcggcgtta gcttctgcgt cgcgtaccat tttctggatt tcatcttcgt
     2041 tcagaccaga agaagccttg atggtgatct tctgctcttt accgctgttt ttatctttcg
     2101 cggaaacgtg caggataccg tcagcatcga tatcgaaggt aacttcgatc tgcggcatgc
     2161 cgcgcggtgc cgggttgata ccatccaggt tgaactgacc cagagatttg ttatcagccg
     2221 cacgtttacg ttcaccctgc agcacatgga tggttaccgc agactggttg tcttcagcgg
     2281 tagagaacac ctggctgtgc ttggtcggga tagtggtgtt tttcgcgatc agcgtcgtca
     2341 tcacaccgcc catggtttcg atacccagag acagcggggt aacgtccagc agcagtacgt
     2401 ctttcacgtc accagtcaga acaccaccct gaacagcagc accgattgct acagcttcgt
     2461 ccgggttaac gtctttacgc ggctctttac caaagaactc agcaactttc ttctgaacca
     2521 ttggcatacg agtctgacca ccaacgagga taacgtcgtc gatatcagat acggacaggc
     2581 cagcgtcctg cagtgcaact ttcagcggct caatggaacg gtttaccaga tcttcaacca
     2641 ggctttccag tttcgcacga gtcactttga tgttcatgtg tttcggaccg gtcgcatctg
     2701 cagtgatgta cggcaggtta acgtcggtct gctgagcgga agacagttcg attttcgctt
     2761 tttctgccgc ttctttcagg cgctgcattg ccagcggatc gttgcgcagg tcaatgccct
     2821 gatctttctt gaattcttca accaggtagt tgatcagacg gctgtcgaag tcttcaccac
     2881 ccaggtgggt atcaccgttg gtagccagaa cttcgaaggt tttttcgccg tcaacttcgt
     2941 cgatttcgat aatagaaata tcgaaagtac caccacccag gtcataaacc gcgatagtac
     3001 ggttgccagt acctttgtcc agaccgtaag ccagcgcagc tgcggtcggt tcgttgatga
     3061 tacgttttac ttccagacca gcgatacggc ctgcgtcttt ggttgcctga cgctgagcat
     3121 cgttaaagta tgccggtacg gtgataacag cttcagttac cggttcaccc aagtaatctt
     3181 cagcggtttt cttcattttt ttcagcactt cagcagagat ctgcggcggt gccattttct
     3241 ggcctttaac ttcgacccat gcgtcgccgt tatcagcagc aataattttg aacggcatga
     3301 tggaaacatc acgctgtact tcttcgtcct ggaagcggcg gccaatcagg cgtttaatcg
     3361 caaacagggt gttttgcggg ttcgtcactg cctgacgttt agccggctga ccaaccagag
     3421 tttcaccatc ctgggtatag gcaatgatag aaggcgtggt gcgatcgcct tcggcgttct
     3481 ccagcacgcg aggagtggtg ccatccataa tcgctacaca agagttggta gtacccaggt
     3541 cgataccaat tattttaccc atctaaacgt ctccactata tattcggtca tcatgtggtt
     3601 gtgagtctgt aataggggcg aaacgtctgg tttcaactgc ccaatttcat ttttttgcag
     3661 actcactcac tgcggttgac tactaaatgg ggtcgtaacc cacgtcatca agggggagat
     3721 gcaaaaattt tttgtgcttt tgcgcagaaa tttcgcgata tagcaggaca agacgtgaaa
     3781 ctgacagcaa ttttattcat ataaagaatg aatcgcagcc aagacattca tcaaaaaatt
     3841 gtaataaaaa gaaaacatta cgcacctgaa tcttctcttt atcagcagta aactagtggg
     3901 tatccatccc cctacctctc cccactaaga gaatccttat gaaatccgtt tttacgattt
     3961 ccgccagcct ggcgattagt ctgatgctgt gctgcacggc gcaggcaaac gaccataaaa
     4021 tcctcggcgt cattgcaatg ccgcgtaacg aaaccaacga tctggcgttg aaacttcctg
     4081 tttgccgaat tgttaaacgc atacaactct ctgccgacca tggcgattta cagttaagcg
     4141 gtgcatcggt ttatttcaaa gccgcccgta gcgccagtca gagcctgaat attccttcag
     4201 aaataaaaga aaggcaaacc actgactgga tcaacattaa cagcgataac gacaataaac
     4261 gctgcgtctc aaaaatcacc ttttccggtc atacggtgaa ctcatcggat atggccacgc
     4321 tgaaaattat cggcgacgac taacgccaga atatagaagc cacaaaaaat gaatgttaat
     4381 tacctgaatg attcagatct ggattttctc cagcattgca gtgaggaaca gttggcaaat
     4441 ttcgcccgat tgctcaccca taatgaaaaa ggcaaaactc gcctctccag cgtactgatg
     4501 cgcaacgaac tgtttaaatc gatggaaggg catcccgagc aacatcgccg caactggcag
     4561 ctgattgctg gagaattaca gcattttggt ggcgatagta tcgccaacaa actgcgcgga
     4621 cacggtaaat tgtatcgggc cattttgctc gatgtttcaa aacgattgaa gctgaaagcc
     4681 gacaaagaga tgtctacgtt tgagattgag caacagttac tggaacaatt tctgcgtaac
     4741 acctggaaga atatggacga ggaacataag caggagtttc tgcacgcgat cgatgccagg
     4801 gtgaatgagc tggaagagct gctgccgctg ctgatgaaag acaaattatt ggcaaaaggc
     4861 gtgtcgcatt tgctttccag ccaactgacc cgcattttac gcacccacgc agcaatgagc
     4921 gtacttgggc atggtttgct gcgcggcgcg gggctgggag gccctgtagg tgcggcacta
     4981 aatggggtta aagcggtcag cggcagcgcc tatcgcgtga cgattccagc cgtactgcaa
     5041 atcgcctgcc tgcgccggat ggttagcgcc actcaggtct gataccggaa accggggaaa
     5101 attttcattt tcccctcaaa agatcgtaga cactgcccca ctggctgatt attatgccgc
     5161 gccctgaaaa cactacagtt attcagggaa attatttcac cattcattcg atgatgattt
     5221 ttgaggaatt atgggcaaca ctaagttggc taatccggca ccgctgggcc tgatgggctt
     5281 cggcatgacc accattctgc ttaacctgca caacgtgggt tatttcgctc tggacggtat
     5341 tattcttgcc atgggcattt tctacggcgg catcgcgcaa atttttgctg gtctgctgga
     5401 gtacaaaaaa ggcaacactt tcggtttaac cgcattcacc tcttacggtt ctttctggct
     5461 gacgctggtt gcgattctgc tgatgccgaa actgggtctg accgatgcgc caaatgcaca
     5521 gttccttggt gtctatctgg gtctgtgggg cgtatttacg ctgtttatgt tcttcggcac
     5581 gctgaaaggc gcacgcgttc tgcaattcgt tttctttagc ctgaccgtgc tgtttgccct
     5641 gctggcgatc ggtaacattg ccggtaacgc cgcaatcatc cactttgccg gctggattgg
     5701 gctgatctgc ggtgccagcg caatctatct ggcgatgggt gaagtactga acgagcagtt
     5761 tggtcgcacc gttctgccga ttggtgaatc ccactaattg ttttgagatc cccccgctcg
     5821 gggggatttt tttattcgct aacgtcgcgt cttgcactct tcggtctgaa tgctgcaacc
     5881 acttccggtg ccgtttcaac gtatggccct tccagcaact gaatgcagta cggtacgctg
     5941 gcaaaaatac cgtgtaccac aacgttaccc gcagcgtcct tcacaccttc cagcgtctct
     6001 ttaatagact tcggctgacc gggtaagtta aggatcagcg cctgtttgcg aatcacacca
     6061 acctgacgcg aaaggatcgc agttggtaca aaatgcaggc ttatctggcg catctgttca
     6121 ccaaagccag gcatctcgcg gtccgctact gccagcgtcg catcgggcgt tacgtcacga
     6181 cgcgccgggc cagttccgcc cgtggtgagc accagatggc aactcatttc atccactagc
     6241 tcacacaacg tttgctcgat gatcgcctgc tcatcgggga ttaagcgggt ttccagttca
     6301 aacggcgtgg ttagcgccga tgtcagccat tcttccagcg cagggatgcc tttatcctga
     6361 taaacgccgc tggatgcgcg atcagagatg gaaactaagc caatacgtaa agtattcatc
     6421 aagatttttc cgtttaaaca gtctcgttaa acagaatgat acactgcgaa gggagtgaca
     6481 gacaggcttc agaagaggta gcgtgaccga cttcccggtc acgctaagaa tgattacagc
     6541 agatcgccga tcattttttc cagtttttcc tggtcaatag caaacttacg gataccttcc
     6601 gccagtttat ctactgccat tggatcctgg ttgtgctgcc acaggaactc ggactcagtg
     6661 atacgcgccg gacgcgcttt cacttcgccg gtgtaagaca gtttacgttc gatagcccct
     6721 tcgctctcag ccagctcttt cagcagtgta ggtgcgatgg taagacggtc gcagcctgcc
     6781 agttccagaa tttcgccgat gttacggaag cttgcgccca taaccacggt ttcataaccg
     6841 tgctctttgt agtactggta gatttcagat acagaaacca cgcccggatc ttctgccgga
     6901 gcgtactctt tcttatcagt attcgctttg taccagtcaa gaatacggcc aacaaatggc
     6961 gagatcagga acacgcctgc ttccgcacaa gcacgagcct gagcgaagga gaacagcagg
     7021 gtcaggttac agttgatgcc ttctttttcc agctgttctg cagcacggat accctgccag
     7081 gtagaagcca gtttgatcag aatacgatcg ttgctaatac cagcatcgtt gtagagtttg
     7141 atcaggcgtt ttgctttcgc aattgacgct tcggtgtcat aggaaagacg cgcatcaact
     7201 tcagttgaga tacgacccgg aaccagtttc aggatttcca gaccaatatt tactgccagt
     7261 ttgtcggtcg cgtccacgat ctgctgcgcg cgatcgttgc tctgctgttt cgcccaggcg
     7321 acagcatcat caatcaactt acggtattcc ggaatctgcg ctgcgttaag aatgagagaa
     7381 gggttggttg tggcatcctg cggttgatac agcttcattg ccgcgatgtc cccagtgtcg
     7441 gccactacgg tggtgtactg acgaagggag gtcaatttgt ccgtcatgat agtatttctc
     7501 tttaaacagc ttgttagggg gatgtaaccg gtctgccctg atgatatcac gacgcaacgt
     7561 cagcgcaacc gccgacaaga cggttatcgc caggcgagac tgtttcggat ttctgacact
     7621 gcgttgatat cgcccgccat ttttatacaa aacctcatgt atgctacgca gaagttatca
     7681 agtacctcgt agcgtatata cttcttaaac aattggtaac gtttacacag gaaagtcatc
     7741 gcgaccggca ataagaggga tatgcatgcc agattttttc tcctttatta acagcgtcct
     7801 ttggggatcg gtaatgattt acctgctctt cggcgcaggt tgttggttca cttttcgcac
     7861 cggatttgtg cagtttcgct acatccgcca gtttggcaaa agtcttaaaa atagcattca
     7921 tccacagcca ggcggtttaa cctcatttca gtcattgtgt accagtcttg cggcgcgcgt
     7981 gggtagcggc aacctggccg gcgttgcgct ggcaattacc gccggtggac ctggagccgt
     8041 cttttggatg tgggttgccg cgtttatcgg catggcgacc tcgtttgccg aatgttctct
     8101 ggcacaactt tataaagaac gtgacgtcaa tggacagttt cgtggcggac cggcatggta
     8161 tatggcgcgc gggctgggga tgcgttggat gggcgttctg ttcgccgtct ttttgctcat
     8221 cgcctatggc ataattttca gcggagttca ggcgaacgcc gttgcccgcg ccctaagttt
     8281 ttcttttgat tttcctccgc tggtgacagg cattattctc gctgtctttg ctctgctggc
     8341 aatcactcgc ggtcttcatg gcgtcgcccg gctcatgcag gggtttgtcc cgttgatggc
     8401 gataatctgg gtgctgacca gcatggtgat ttgcgtaata aatatcgggc aacttcccca
     8461 cgtcatttgg tctatttttg agagtgcttt tggctggcag gaagcggcag gcggcgcggc
     8521 gggatatacc ttaagccagg cgattactaa cggttttcag cgcagtatgt tttccaatga
     8581 ggcgggaatg ggttcgacgc caaacgcggc agcggcagcg gcgtcctggc ctccgcatcc
     8641 ggcagcgcaa gggattgtcc agatgattgg catttttatc gacaccctgg tcatctgtac
     8701 ggcaagcgcc atgctgatat tactggcggg taacgacaca acctacatgc cgctggaagg
     8761 tattcagctt atccagaagg cgatgcgggt gctaatgggt tcctggggtg ctgagtttgt
     8821 taccctcgtg gttattctgt ttgccttcag ctccatcgtt gccaactaca tttatgccga
     8881 aaacaatctc ttctttttac gcctgaacaa ccctaaagcg atctggtgtt tgcggatctg
     8941 caccttcgca acggtcatcg gcggcacctt gctaagcctt ccgctgatgt ggcaactggc
     9001 agatatcata atggcctgca tggctattac caatttgacc gccattttac tgctctcgcc
     9061 tgtggttcat accattgcca gtgattatct acgccagcgt aaactcggcg tgcgcccggt
     9121 gtttgatccg ttgcgttatc cggatatcgg tcgccagctt tctccggacg cgtgggatga
     9181 tgtttcgcag gagtaatcac aactatcgat caactcattc tctttttttg ctaaagtcgg
     9241 cataaatttc ctgcaaggac tggatatgct gattcttatt tcacctgcga aaacgcttga
     9301 ttaccaaagc ccgttgacca ccacgcgcta tacgctgccg gagctgttag acaattccca
     9361 gcagttgatc catgaagcgc ggaaactgac gcctccgcag attagcacgc tgatgcgcat
     9421 cagcgacaaa ctggcgggta tcaacgccgc tcgctttcat gactggcagc cagatttcac
     9481 gccggagaat gcccgccagg cgattctggc gtttaaaggt gatgtctaca ccggcttgca
     9541 ggccgaaacc ttcagcgaag acgatttcga ttttgcccaa cagcatttgc gaatgctttc
     9601 cggcttgtat ggcgtactgc gcccgctcga tttaatgcag ccttatcgtc tggaaatggg
     9661 gatccgtctt gagaatgccc gagggaaaga tctgtatcaa ttctggggag atatcatcac
     9721 caacaagctg aacgaggcgc tcgcagcaca aggcgataat gtggtgatta atctggcgtc
     9781 agatgagtat tttaaatcgg tgaagccgaa gaaattgaat gccgagatta tcaagccggt
     9841 gttcctcgat gagaagaacg gcaaatttaa gataatcagc ttctacgcta agaaagcacg
     9901 cggtctgatg agtcgtttta ttattgaaaa tcggctgacc aaaccagagc aactgactgg
     9961 ttttaatagc gaaggttact tctttgatga agattcctcc agcaatggcg aactggtgtt
    10021 taaacgctac gagcagcgtt aagtttgtag tcaatgccgg atgcggcgtg aacgccttat
    10081 ccggcctaca aaagcatgca gattcaatat attgcagaat tcacgtaggc ctgataagac
    10141 gcgccagcgt cgcatcaggt gctgaatgcc ggattgttac ccggcatttg tcatttagcg
    10201 gtgatgtttg cctggaccat gaccgccgtg atgatcatga ggagctttct tatggtggcg
    10261 cggcggtggc ggcggtccgt gtgggtgcca gcgattgcct cgccattcat aatgttgttt
    10321 ccaccagccg tggtcgcgcc agtgacctcc atcccagtaa tagccacgat tatcacgatc
    10381 gcctatctgt aattttactg acgggactaa cgtaatttcc gcagcctgtg ctgccatggg
    10441 agcgaccaga accagggaaa gtgcgagtac gatagattgc atctttttca caggttactc
    10501 cttttatcat tgcccgttat gggtcgatat ccggagatta cgcgatgaga acgacggttg
    10561 ttattctccg caatcctaat ctctaagtta ccgcatttat tgagaatttc tcctttttcc
    10621 ctgtcgtttt tctccataat ttcttcataa aaaagccggg ctgcataaaa gcaaacccgg
    10681 cctgattgag ataatgaata gattttactg atgattcatc atcaatttac gcaacgcagc
    10741 aaaatcggcg ggcagattat gtgaaagcaa gggtaaatca gcacgttctg ccagctcttt
    10801 tggcagatcc aacgtttcac cgagaatcgc ttccacgctc tctttaaatt tcgccggatg
    10861 cgcggtgccg aggaacaagc catattcgcc tggattcaac tggtcacgca gcgcacgata
    10921 agctacggcg gcgtgcggct ccgaggtgta gcccagttct tttaactcac gcattgtctg
    10981 ttgcgtggtt tcatcatcca cggctgcata acccagctct ttcagttgcc agattttgcg
    11041 gcggaacaac tcttccacac gcggccagtt gttcggctgg ctcacgtcca tcgcgttgga
    11101 taacgtcgcc tgagtcgctt tgggtgacca ctgaccgtcg tgcaggaaac gtggcacggt
    11161 atcgttcacg ttggtcgcag caataaaacg tttcaccggc agaccgagtg acttcgccag
    11221 cagacccgcc gtcaaatcgc cgaagtttcc gcttggcacc gagacaacca gctggttgcg
    11281 cgcttcctgc ggcagctgcg caactgcttc aaagtagtag caaatctgcg ccagcaaacg
    11341 gctgatgtta atcgagttag ctgagtttaa ccccagcgcc actttcagct cttcatcatc
    11401 aaacgcctgc ttcaccagcg cctgacaggc atcgaaatcg ccgtcgatgg caacagtttc
    11461 gatattgccg cccaatgtac agaacagttt ttcttgcagt ggactgattt tgcctcgtgg
    11521 atagaggata accactttca cattcggtaa accgtagaaa gcatgagcta ctgccgctcc
    11581 ggtatcaccg gaggtcgcgg tcagaatggt cactggctta tcgcccgcaa tatgggtcag
    11641 catttgtgcc ataaagcgac cgccgaaatc tttaaatgcc agcgttggcc cgtggaacaa
    11701 ttccagacaa ccgacatcgc tttcaacatt ggcgaccgga gccgggaagg caaacgccgc
    11761 gcgcacgcgc tcttccagga tttcctgcgg gatttcatca ccaataaacg ccgagaggat
    11821 cttcgcactg cgggtgacaa aatccagctt cagcatctca tcaatttcag tcaggctgaa
    11881 ttccggcagg tcgtgcggaa aaaacagccc ctgatttttg cccaacccct gggttacggc
    11941 ttgcgcaaag ctgacctgct cattgtgatc tttcagattg tagagtttca tttagttttc
    12001 cagtactcgt gcgcccgccg tatccagccg gcaaatatga acaaaacctt cctgattttg
    12061 caggtagttc ttacccaacc agtcggcaac gcgctgggcg gtatccggct tgtcacacag
    12121 agcgaacaag gtcgggccgg agccggagat accgctcgct accgcgccga tttccgcgac
    12181 cgcctgccgc gcctgccgga agccaggcag taaccgttca cggtagggtt ctgcgataac
    12241 atctttcatc agcttcgcgg caagctcagg ctgacgggaa tagcaggcgt gaatgaagcc
    12301 tgccagatgt cgcccgtgcg caatgcaatc ctggcggcga tactgcgccg gtaaaatagc
    12361 ccgggcttct gccgtcgaga ctttaatccc cggatacgcc agcacccaca gccactcatc
    12421 aaaccctggc acttgctggc tgatgatgtc gttttcttcg atcatcaact gcattccacc
    12481 aagaaaacac ggtgccacgt tgtcgtaatg aatgctgccg gagatacgtc cttccagctc
    12541 gcccatcaaa gccagcaaac gggtgtcatt aagtggcttg ccgcagtgtt cattcatcgc
    12601 catcagcgcc gcgaccaccg aacaggcgct ggagcctaag cccgagccga tcggcatatt
    12661 cttttccaga gtcatcgcca ctggaatttg cttcccaagc tcctggcaaa aacgctccca
    12721 gcactgataa acgatatttt cccgtggttc tgacggcagc ttatcggcaa agcgtccgag
    12781 gttgttgaga ccgaatgtct ctgccgcctc aaccgtgact acatctccga gcaatgcacc
    12841 atcaacgggt gtcaccgccg ccccgagcac atcaaacccg acgctcatat tggcactgga
    12901 agccggggca taaactttaa ccatgtcaga ctcctaactt ccatgagagg gtacgtagca
    12961 gatcggcaaa gacaccggca gcggtaacgt cattgcccgc accatatccg cgcagcacca
    13021 acggcagcgg ctgataatag tggctataga aggccagggc gttttcgcca tttttcactt
    13081 tgaacagcgg atcattacca tccacttcgg caatcttcac gcggcagacg ccatcttcat
    13141 caatattgcc aacatagcgc aaaacttttc cttcatcacg ggccttagcc acgcgcgcgg
    13201 caaagagatc gtcgagctgt gacagattcg ccataaaagc ggcaacatca ccctcggcgt
    13261 taaactctgc gggcagcact ggttcaattt caatatccgc cagctccagt tcacgtcccg
    13321 tttcacgagc gagaatcaat agcttacgcg ctacatccat accagaaaga tcatctcgcg
    13381 gatccggttc ggtataaccc atttcccgcg ccagagtagt cgcctcggag aaactcatgc
    13441 cttcgtctaa cttgccgaag atataagaaa gcgaacctga aagaatgccg gagaacttca
    13501 tcaattcatc accagcattg agcagatttt gcaggttctc aataaccggt aatccagccc
    13561 caacgttggt gtcatagagg aatttacgcc gcgatttttc cgccgcatga cgcaacagat
    13621 ggtagtaatc catcgacgag gtgttggcct ttttgttcgg cgtgacaacg tggaaacctt
    13681 cgcgcaagaa gtcggcatat tgatccgcca ctgcctggct ggaggtgcag tcaacaatga
    13741 ccgggttcag cagatgatat tctttcacga ggcgaattaa gcgcccgaga ttaaacggct
    13801 ctttggcttg cgccagttct tcctgccagt tttccagatt taggccatgc acattggtga
    13861 gcagtgcctt cgagttggca acaccgcaga cacgtaagtc gatatgttta ttcttcagcc
    13921 agctttgttg acgcttcagt tgctccagca gcgcaccgcc aacgccaccg acgccaatca
    13981 caaacacttc gataacctga tcggtattga acagcatctg atgagtaacg cgcacgccag
    14041 tggtcgcatc atcgttattt accacgacag agattgagcg ttcagaagat ccctgagcaa
    14101 tggcgacaat gttgatattg gcgcgggcca gcgcggcaaa gaatttcgcc gagatcccac
    14161 gcaaggtgcg cataccatca cctaccaccg agataatggc cagccgttcc gtcaccgcca
    14221 gcggctccag taagccttct ttcagttcca ggtagaactc ttcctgcatt gcccgttcag
    14281 cccgcacaca gtcgctttgc ggaacgcaga aactgatact gtattcggaa gatgattgcg
    14341 taatcagcac cacggaaata cgggcgcgtg acatcgcggc aaagacgcgc gccgccatgc
    14401 cgaccattcc tttcatcccc gggccggaaa cgctgaacat tgccatgtta ttcagattgg
    14461 aaatgccctt gaccggtaat tcgtcttcat cacgactggc accaatgagc gtacctggtg
    14521 cttgaggatt tccggtattt ttaatcaggc aagggatctg gaactgggcg atgggggtaa
    14581 tggtgcgggg gtgaagaact ttagcgccga agtaggaaag ctccatcgct tcctggtagg
    14641 acatcgactt caacaacctc gcatcgggca cctgacgcgg gtcgcaggta tagaccccgt
    14701 caacgtccgt ccaaatctcg caacaatcgg cgcgtaaaca ggcagccagc accgccgcgg
    14761 agtagtcgga accgttgcgt ccaagtacca ccagttcgcc tttttcatta ccggcggtga
    14821 aacctgccat caacaccatg tgatcagccg gaatgcggct tgccgcaata cggcgggtgg
    14881 actcagcaat atcgacggta gattcgaggt aatgccccac tgccagcagt ttttcgaccg
    14941 gatcgataac ggtaacgttg tgaccacgcg cttctaacac gccggccata atggcgatcg
    15001 acattttctc gccacggcaa atcagcgcag cgttgatgct atccgggcac tgccccaaca
    15061 aactaatgcc atgcaggaca tgttttattt gggcaaattc ctgatcgacg aaagttttca
    15121 attgcgccag cgggaacccc ggctgggcgg cggcgagtcc cgtcaaaagt tcggcaaaaa
    15181 tacgttcggc atcgctgata ttgggtaaag catcctggcc gctaatggtt ttttcaatca
    15241 tcgccaccag gtggttggtg attttggcgg gggcagagag gacggtggcc acctgcccct
    15301 gcctggcatt gctttccaga atatcggcaa cacgcagaaa acgttctgca tttgccactg
    15361 atgtaccgcc gaacttcaac actcgcatgg ttgttacctc gttacctttg gtcgaaaaaa
    15421 aagcccgcac tgtcaggtgc gggctttttt ctgtgtttcc tgtacgcgtc agcccgcacc
    15481 gttacctgtg gtaatggtga tggtggtggt aatggtggtg ctaatgcgtt tcatggatgt
    15541 tgtgtactct gtaattttta tctgtctgtg cgctatgcct atattggtta aagtatttag
    15601 tgacctaagt caataaaatt ttaatttact cacggcaggt aaccagttca gaagctgcta
    15661 tcagacactc tttttttaat ccacacagag acatattgcc cgttgcagtc agaatgaaaa
    15721 gctgaaaaat acttactaag gcgtttttta tttggtgata tttttttcaa tatcatgcag
    15781 caaacggtgc aacattgccg tgtctcgttg ctctaaaagc cccaggcgtt gttgtaacca
    15841 gtcgaccagt tttatgtcat ctgccactgc cagagtcgtc agcaatgcca tgactcgttc
    15901 gcgtaaagct tgcagttgat gttggtctgc cgttgtgtca cttttcgccg gttgttgtat
    15961 taatgttgct aattgatagc aatagaccat caccgcctgc cccagattga gcgaaggata
    16021 atccgccacc atcggcacac cagtaagaac gtcagccaac gctaactctt cgttagtcag
    16081 cccggaatct tcgcgaccaa acaccagcgc ggcatggctc atccatgaag atttttcctc
    16141 taacagcggc accagttcta ctggcgtggc gtagtaatga tatttcgccc gactgcgcgc
    16201 agtggtggcg acagtgaaat cgacatcgtg taacgattca accagtgtcg ggaaaacttt
    16261 aatattatca ataatatcac cagatccatg tgcgacccag cgggtggctg gctccagatg
    16321 tgcctgacta tcgacaatcc gcagttcgct aaaacccatc gttttcattg cccgcgccgc
    16381 tgccccaata ttttctgctc tggcgggtgc gaccagaata atcgttatac gcatattgcc
    16441 actcttcttg accaaataac cgcgaacagg gtgatcactg tcaacttatt acgcggtgcg
    16501 aatttacaaa ttcttaacgt aagtcgcaga aaaagccctt tacttagctt aaaaaaggct
    16561 aaactatttc ctgactgtac taacggttg
//
LOCUS       DAPHDW010000098        16583 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.099, whole genome shotgun sequence.
ACCESSION   DAPHDW010000098 DAPHDW010000000
VERSION     DAPHDW010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16583
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.099"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            391..873
                     /locus_tag="RH057_003137"
     CDS             391..873
                     /locus_tag="RH057_003137"
                     /inference="COORDINATES: protein motif:HMM:NF024954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3129469.1"
                     /translation="MGQQRGRLSPFLERCKIISLVHEAVINGARKSQACRCIGISIRT
                     LQRWTNGNVITTDRRSDTVNRSPRNKLSEAERLRILELCNSPEFASLPPNVIVPTLAD
                     RGIYIASESSFYRVLKSANQLKKRSRECQYKRPDVLRAVAPNQVWSWDISYLPSWVRG
                     "
     gene            1050..1238
                     /locus_tag="RH057_003138"
     CDS             1050..1238
                     /locus_tag="RH057_003138"
                     /inference="COORDINATES: protein motif:HMM:NF025063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3129470.1"
                     /translation="MHVVGENVCAGVLSSYSRPRVSNDNPYSESLFRTLKYCPWWPEN
                     GFYTIDDARVWVNRFVNW"
     gene            1534..1879
                     /locus_tag="RH057_003139"
                     /pseudo
     CDS             1534..1879
                     /locus_tag="RH057_003139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2075..2935)
                     /locus_tag="RH057_003140"
     CDS             complement(2075..2935)
                     /locus_tag="RH057_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000205482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HDV3129471.1"
                     /translation="MTTATSLNELQKQIRDRYDSLSKRLQQVSRYVLDNTNSVAFDTV
                     AVIAERADVPPSTLIRFANAFDFTGFNEMKQLFRMHLVEETASYSDRARLFREMESKA
                     VPETPLDILQEFASSNSQALQQLAARAEPEMLEHAVQLLAYADTIYIAGLRRSFSIAA
                     YLTYALSHLECRPILLDGMGGMLREQISRIKACDIVVSISFSPYAEETVMVSKTAASV
                     GARQIVITDSQISPLATFSDLCFVVKEAQVDAFRSQSATLCLVQSLVVALAYRLGDKK
                     HNNTQENSNQ"
     gene            3226..4731
                     /locus_tag="RH057_003141"
     CDS             3226..4731
                     /locus_tag="RH057_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-acylating methylmalonate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3129472.1"
                     /translation="METVANFIHGECVTGSGQRIQAIFNPATGEQIRQVVMSTAQETE
                     QAIAAAQLAFPAWARFAPLKRARVMFRFKALLEENMERLARIISEEHGKVFSDAVGEL
                     TRGLEVVEFACGIPHLQKGEHSANVGTGVDSHSLMQPLGVCAGITPFNFPAMVPMWMF
                     PIALATGNTFVLKPSEKDPSLALALAKLLKEAGLPDGVFNVVQGDKESVDVLLTDPRV
                     QAVSFVGSTPIAEYMYLTASAHGKRCQALGGAKNHCILMPDADMDMATNAIMGAAYGA
                     AGERCMALSVVVAVGDKTADELCARLEKKIASLRVGPGLNQTPENEMGPLISAAHRSK
                     VLGYIDSGEAQGANLRVDGRGLRVKGHEQGYFVGPTLFDNVTPEMTIYKEEIFGPVLS
                     VVRVADYASAVDLINRHEYGNGSAIFTRDGGCARRFCEEVQAGMVGVNVPIPVPMAFH
                     CFGGWKRSIFGALNVHGNDGVRFYTRMKTITARWPEMQQETGAAFSMPTLG"
     gene            4743..6668
                     /gene="iolD"
                     /locus_tag="RH057_003142"
     CDS             4743..6668
                     /gene="iolD"
                     /locus_tag="RH057_003142"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HDV3129473.1"
                     /translation="MQTERMTMAQALVRFLNQQYVSVDGKETPFVEGVATIFGHGNVL
                     GIGQALEQDPGHLQVMQGCNEQGIAHMATGFAKQHRRQRIFAVTSSVGPGAANMVTAA
                     ATATANRIPLLLLPGDIYACRQPDPVLQQIEQYHDLSISTNDCFRPVSRYWDRINRPE
                     QLMSAMLSAMRTLTDPANTGAVTICLPQDVQGEAWDYPLSFFARRVHHIERRPPDPQR
                     LAQAHALISRKRRPLVVCGGGVRYSGAHEALREFVEVLQLPFAETQAGKGALVSDHPL
                     NLGGVGVTGGMAANQLAPQADLVIGIGTRLTDFTTGSKALFSHPDVEFLLLNVAEFDS
                     LKLDATALIADARTGLTALTHALKDYRSGWGEAIAHAKSAWHDECCRLWERQWHPDDV
                     PEVAGHLDAQLMEYGETLNTHLTQTRVLGLINQHIEDNAIVVGAAGSLPGDLQRLWQV
                     KTPDSYHLEYGYSCMGYEVAAAIGAKLAKPQQPVYAMVGDGSYMMLHSELQTAVQEGI
                     KVTILLFDNASFGCINNLQMGHGMGSFGTENRYRNPKTGQLDGPLVKVDFAQNAESYG
                     CRAWRVHNEKSLLAALEASRAHPGPTLLDIKVLPKTMTHDYASWWRTGNAQVAESPAV
                     RQAAEQTQAQVKKARQY"
     gene            6953..7939
                     /gene="iolG"
                     /locus_tag="RH057_003143"
     CDS             6953..7939
                     /gene="iolG"
                     /locus_tag="RH057_003143"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HDV3129474.1"
                     /translation="MFNIALLGAGRIGQVHAANIASHSATTLWSVVDPNQEFATRLAT
                     QYQARQQSLDEAMTDPNVHAVLIASATDTHADLIEMAARYGKAIFCEKPVHLDLARVR
                     DCLKVVKEYDVPLFIGFNRRFDPQFRRVKTDTQAGRIGKPESLLIISRDPSPPPAEYV
                     RVSGGMFRDMTIHDFDMARFIMGEEPVSVYAQGSNLVDPAIGGAGDIDTAFIVLKYAS
                     GAMATIVNSRRSSYGYDQRLELHGSEGLLCAGNILENQVQHYGKQGCTSALPEHFFLQ
                     RYKSAYAAEWEHFVAVLRGEAVPDCSGDDGERALYLADKALESLRCQREIVL"
     gene            7971..8900
                     /locus_tag="RH057_003144"
     CDS             7971..8900
                     /locus_tag="RH057_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015871439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HDV3129475.1"
                     /translation="MKKLILAALIAMTSGAAIAENEQIVFSTPNLAMPFEVHMQRTAV
                     KAAKEMGVKLQVLDGQGSSPKQVADLENAITRGAQGFIVSPNDVNAVSSAVDEIQDTK
                     LPVVTLDRSVDSQKKVPHFGANNYKGGQAIGDFVKTKFPNGAEIILLTGQPGSSSNIE
                     RTKGIRDSLKAGGDMYKIVADQTGNWMRSEGMRIVESVLPSLPKRPQVILSANDDMAL
                     GAIEALQSQGVKPGEILVTGFDAVPEALARVRDGWLAVTADQRPGFAVTTAMSQLVAN
                     VREKKTITGADYPPTLITKENLQQAERIAEAGN"
     gene            8952..10499
                     /locus_tag="RH057_003145"
     CDS             8952..10499
                     /locus_tag="RH057_003145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HDV3129476.1"
                     /translation="MSQPLLKVTDLAKSFSGVWALSSAQLTVGTGEIHALLGENGAGK
                     STLLKALAGAQPQTRGEIWFNGETLPVDDSPVERQNKGIITIYQEFNLLPNMTVAENM
                     FLGREPRKRNLIVDEKAVNQEAQVILDYLQLNVAPTTPVARLSVAQQQMVEIARALTL
                     NARLIIMDEPSAALSDSEVESLHRVVRELKNRGVSIIYVTHRLHEVFQLCDRFTVFQD
                     GRFTGTGAVTETNVEQLIRLMVGRDVAFNRRPASETHHEDKPVRLSVKGLSREKPPLD
                     PHGIALHDISFHVHAGEVLGIAGLVGAGRTEVARCLFGADAFTTGSFELDGVPYQPRD
                     PLYALEQGLALVPEDRKKEGAVLGLSIRDNLSLSCLSSLLQWRWFVNTRKEDDLIESY
                     RQALQIKMVNSTQEVRKLSGGNQQKVILARCMALNPRVLIVDEPTRGIDVGTKSEVHQ
                     VLFDMAKKGVAVIVISSDLPEVMAVSDRIITLSEGRLTGEIHGDDANEERLMTMMAIN
                     HNALNAA"
     gene            10511..11539
                     /locus_tag="RH057_003146"
     CDS             10511..11539
                     /locus_tag="RH057_003146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003826102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3129477.1"
                     /translation="MTQMIPKTQVPVKRAGRIDPIAFFERFGVLIFMILLLIFFQSQN
                     SNFFSERNIFNILTEVSIYGIMAVGMTFVILTAGIDLSVGSILAVCAMTAAYVIKGDN
                     FTTVDPNAWGGMSWLIGLGICLTMGTAIGFLHGLGVTRLRLPPFIVTLGGMTIWRGLT
                     LVINDGAPIAGFDQGYRWWGRGELLGISIPIWIFAIVAIGGYLALHKTRWGRFVYAIG
                     GNPEAARLAGVNVKRVLVSVYVLIGCLAGLAGFILSARLGSAEAVAGISFELRVIASV
                     VIGGTSLMGGYGRIGGTIIGSIIMGILINGLVLMNVSAYYQQIITGLIIVLAVAFDTY
                     AKSRRGAL"
     gene            11548..12681
                     /locus_tag="RH057_003147"
     CDS             11548..12681
                     /locus_tag="RH057_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013098253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HDV3129478.1"
                     /translation="MKEVRIGLIGTGYIGKAHAIAYAQAPTVFNLRGKLVREMVAEVN
                     PALAAERAQAFGFNRSTGDWRVLVADPAIDVVDICSPNHLHKEMALEAIRHGKHVYSE
                     KPLALNAHDAREMVDAAKRAGVKTLVGFNYMKNPTAQLAKEIIARGEIGEVIHFYGTH
                     NEDYMADPLSPIHWHCFKETAGLGALGDLAAHIVNMAQYLVGEIEQVCGDLKIVVPAR
                     PAKAGSSEMVTVENEDQAHAMVRFAGGAQGVIETSRVACGRKMGLSYVITGTKGAISF
                     TQERMAEIKLYLHDDPVNRQGFQTLLVGPAHPDYGAFCMGAGHGIGFNDQKTVEVRDL
                     VDGIATDAPMWPDFEEGWKVSRVLDAIALSHQQGLWLNVNDIV"
     gene            12694..14598
                     /gene="iolC"
                     /locus_tag="RH057_003148"
     CDS             12694..14598
                     /gene="iolC"
                     /locus_tag="RH057_003148"
                     /EC_number="2.7.1.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-2-deoxygluconokinase"
                     /protein_id="HDV3129479.1"
                     /translation="MEKQFDVICMGRVAVDLYSQQIGARLEDVSSFAKYLGGSSGNVA
                     YGTARQGLRSSMLARVGDEHMGRFLREELNQVGCDTSHLITDKERLTALVLLGIKDRD
                     TFPLIFYRDNCADMAITASDVDESYIASARCIAITGTHLSHPQTREAVLTALNYARRH
                     GVRTVLDIDYRPVLWGLTSLGDGETRFIVAEQVTRELQEVLHLFDVVVGTEEEFHIAG
                     GSTDTLQALAQVRHVSKATLVCKRGALGCSVYTDAIPPRLDDGLTVTGVRVEVLNVLG
                     AGDAFMSGLLRGYLNDEGWEQACRYANACGALVVSRHGCAPAMPSKIELDDYLSRAAL
                     VPRPDIDPRLNHLHRVTTRRREWPELCVMAFDHRSQLEDMALQCGASLKRIPALKQLI
                     LQASREAASSAGLEGKAGLLCDGTFGQDALNAITGEGWWIGRPIELPGSRPLEMEHGN
                     IGTQLISWPQEHVVKCLVFFHPEDAHGLRLEQEQKIAEVYHACCQSGHELLLEVILPA
                     TMPRSDELYLRAISRFYNLGIYPDWWKLPPLSAEGWTALSEIIARRDPHCRGVVILGL
                     DAPAEQLRADFKAAAGQALVKGFAVGRTLFGDASRAWLKHDIDDAQLVARIRDNYLQL
                     IAWWRERGHA"
     gene            14610..15500
                     /gene="iolE"
                     /locus_tag="RH057_003149"
     CDS             14610..15500
                     /gene="iolE"
                     /locus_tag="RH057_003149"
                     /EC_number="4.2.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010433880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myo-inosose-2 dehydratase"
                     /protein_id="HDV3129480.1"
                     /translation="MSVQLGINPLTWTNDDLPSLGAETSLETCLSEGKEAGFAGFELG
                     NKFPREARLLGPILQRHDLQLVSGWYSGRLLERSVEDEIAAVQSHLTLLRELGAKVLV
                     FAEVSGCIHGEQQTPVHLRPRFPQECWKEYGEKLTVFARYTQQQGVQIAYHHHMGTVI
                     ESAQDVDNLMLHTGEEVGLLLDTGHLTFAGADPLAVAQRWASRINHVHCKDVRADVLA
                     DVKNRKTSFLDAVLSGVFTVPGDGCVDYPPIMQLLKTQDYHGWLVVEAEQDPAIAHPL
                     TYARLGYNNLSRLARDAGLI"
     gene            15510..16331
                     /gene="iolB"
                     /locus_tag="RH057_003150"
     CDS             15510..16331
                     /gene="iolB"
                     /locus_tag="RH057_003150"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HDV3129481.1"
                     /translation="MSRLLSRWQQPNAEGRTQSVTPESAGWGYVGFEAYELEEGQQLT
                     LPAVSEERCLVLVAGRAFISTPSAQFTDIGERMSPFERIKPWAVYVTPQEAVQVKALT
                     KLELAVCTAPGKGTYPTRLIAPQDIDGEARGNGHNQRYVHNILPEDKPADSLLVVEVW
                     TNEGCTSSYPSHKHDTNNPPQESYLEETYYHRLNPEQGFCMQRVYTDDRTLDECMAVY
                     NRDVVMVPKGYHPVATMAGYDSYYLNVMAGPVRKWIFTWEKEHSWINTEYSAISL"
BASE COUNT         3732 a         4316 c         4832 g         3703 t
ORIGIN      
        1 tttttgttcg taagcgttaa cggaacactg tattgacgtt tatttattgg tgagaactac
       61 gttccttggc aggagttcgt caacacagtt ggaagtctat tccggcagta agctcaggat
      121 atgaagcaga tacgcttctg gaacgattcc gttcagttgg caggtaccga ttcgtccatg
      181 cgcagtgcac cgtgctttcc gctgtaatag ctgccaaagg acacggttat caaataaatc
      241 tggatattga tggaaaatat tcgaacagac agggcattgc gtgacgcaaa aaaataagtt
      301 acgagaactg gagaaagagc ttaagcgcaa agataaagct ctggcggaag ctgcggctct
      361 gcttatgtta agggaaaagt tcaatgccct ctgggacaac agcgaggaag actgtcccct
      421 ttcctggagc ggtgtaaaat tatttctctt gtccacgagg ccgttatcaa cggcgcccgg
      481 aaaagtcagg cttgtaggtg tatcggtatt tctattcgga cacttcagcg ctggactaat
      541 ggcaacgtca tcaccacgga ccgaagatcc gatactgtaa acagatctcc ccgaaataag
      601 ctgtcagaag cggaaagatt gcggatactg gagttgtgca actctccaga gtttgcatcc
      661 ttacctccaa atgtgatcgt accaactctg gcagacaggg gtatttatat cgcttctgaa
      721 tcaagctttt atcgtgtact gaaatcagcc aaccagttga agaaacgctc ccgtgaatgt
      781 cagtataaac gccctgatgt actcagggcc gttgcgccga atcaagtctg gagttgggac
      841 atcagctatt tgccgtcctg ggtcagaggc tagcattttt acctctatat gatcgtcgat
      901 atttacagtc gcaagattat tgctgcagag gtatttgcca gcgaatccgg aaaacatgca
      961 gcagaactac tccagcgggc agtatggaat gaaaaatgtt catccaacaa tctggtactc
     1021 cattctgata atggtggtcc catgcgcagt tgcacgttgt tggcgaaaat gtatgcgctg
     1081 gtgtgctaag ttcttattcc cggccaagag tcagtaatga taatccttac tcagaatctt
     1141 tgttccggac tctcaaatat tgtccctggt ggcctgagaa cggtttctat actattgatg
     1201 atgccagagt atgggtaaac cgttttgtaa actggtaaaa ctttgaacat aagcacagcg
     1261 ggatcaaata cgtcacgcca gccgagcgtc atcaaggtaa tgacatgaaa atactgatag
     1321 ccagaaaagc agtatatcaa ctggcacgcg agcaacatcc tgaacgttgg agcaagcatc
     1381 tacgtaaatt ggactacata tcagaagtct acctgaatcc agaaaaagag gctgcataac
     1441 gtaattttat tacaaaagtc gacaactacc ttgaaaaacg ccgccaatcc gtgatcgaat
     1501 agagtggaat acgcaccact atcatttaaa taagtgaaag tcttgctaca cgtgttcaga
     1561 ctagtcgttg gagaacagat gattgatgtc tcaggatcgg agaaacgcag actgtgcatc
     1621 acacacgcaa aatcgctact gttcaacaga gttttgaacc aggaatgatg gtcttcctga
     1681 ttgcctggca acatggtatg gtggtcagcc cgttatttct ctggcgtatc attaccttga
     1741 aggaagcttc actactgtga cagccgaaga acagggtatt ccggcctctg aacttgctgc
     1801 cgccatgaat aaaattaaag aacttcagcg cctgctaggt aagaaaacga tggtaaataa
     1861 actgtttaaa gaagccgtta tatctgggtt acattaccac ctactagaga aatcttcttc
     1921 gctattaagg attaaggctc tcgttgtacg ctcacatggg gcatgacctt ttagggagat
     1981 agcagtacag ttgatattca atctgaagtc tgtatcgcta ctggcaccga atcatttact
     2041 cttatatgga ctcgggggag gagttcattg gttattattg gttactgttt tcctgggtat
     2101 tattatgttt tttatctcct aaacggtaag ctagcgcgac cactaacgac tgcaccagac
     2161 acaatgttgc cgactgagac cgaaatgcgt ccacctgagc ctctttcacc acaaagcaga
     2221 gatcactgaa cgttgccagt gggctgatct ggctatccgt aatcacaatc tgccgagcgc
     2281 cgacgctggc ggcggttttg ctgaccatca ccgtttcttc cgcgtacggc gagaaactga
     2341 tcgacaccac aatgtcgcac gccttaatac ggctgatctg ttcgcgcagc atgccgccca
     2401 tcccgtcaag cagaataggg cggcactcta ggtggcttaa agcataggta agataagcag
     2461 caatgctaaa ggatctgcgt aatccggcga tgtaaatggt gtcggcgtag gccagtagtt
     2521 gtacggcgtg ctccagcatc tccggttcgg cgcgggcagc caactgctgc agcgcctgcg
     2581 agttcgaact ggcgaactcc tgcaaaatat cgagcggcgt ttcaggtacg gctttgctct
     2641 ccatttcgcg gaacagtcgc gcacgatcgc tgtagctggc ggtttcctcc accaggtgca
     2701 tacgaaataa ctgtttcatt tcgttgaagc cggtgaagtc aaaggcattg gcaaaacgaa
     2761 tcagtgtaga gggtggaaca tcggcacgtt cggctatgac cgcaaccgta tcaaacgcga
     2821 cactgttcgt attatccagt acatagcggg agacctgctg cagccttttg ctcaggctat
     2881 cgtagcgatc gcggatttgc ttttgtagtt cgttcaggct ggttgctgtc gtcatgttat
     2941 ccccctatcc agtctcatga ttctaaacgc aaaaccgcgt cttttaaatg ttggccatcc
     3001 acctggcagc aaaaatgaaa cagaagcttc agtcatttcc tcgcccgaaa ctcctaatag
     3061 ctcaggctga gtcagtgatg ctggctgttc acataaaaat ccatccttga tcacaataat
     3121 cattatttat tttatttcaa cttaaaatgg aatatttgtt tcatatatag tgttcagtac
     3181 ataacgttca ggtaacaatc acgtatcaac agcgagaggt taaggatgga gacggtagcg
     3241 aattttattc acggcgaatg tgtcaccggt tcaggccagc gcattcaggc gatcttcaac
     3301 ccggccactg gcgagcagat tcgccaggtg gtgatgagta cggcacagga aaccgaacag
     3361 gcgattgccg cagcacagtt ggcgtttccc gcgtgggcac gttttgctcc actcaagcgc
     3421 gcgcgtgtga tgttccgctt caaagcgttg cttgaagaga atatggagcg tctggcccgt
     3481 attatcagtg aagaacacgg taaggtcttc tcagatgcag taggtgaact gacccgtggt
     3541 ctggaagtgg tagaattcgc ctgcggcatc ccacatctgc aaaaaggcga acactctgcg
     3601 aacgttggca caggtgtcga cagccactca ctgatgcagc cgctcggggt ctgcgccggg
     3661 atcacgccgt ttaacttccc ggcgatggtg ccaatgtgga tgttcccgat agctctggcg
     3721 acaggcaaca ccttcgtgct gaaaccgtcg gaaaaagatc cgtctctggc gctcgcgctg
     3781 gcgaaactgc tgaaagaggc aggcctgccg gacggcgtat tcaacgtggt gcagggcgat
     3841 aaagaatccg ttgatgtgct cctgaccgac ccgcgcgtgc aggccgtcag ttttgtcggt
     3901 tcaacaccga ttgccgaata catgtacctg actgcgtccg cccacggtaa gcgctgtcag
     3961 gcgctcggcg gtgcgaaaaa ccactgcatt ttaatgccgg atgccgatat ggatatggcg
     4021 acaaacgcca taatgggcgc agcttatggt gcggcaggag aacgctgcat ggcgctttcc
     4081 gtggtggtgg cggttggcga taaaacggct gatgaactct gcgcccggct ggaaaaaaag
     4141 atcgcttcgc tgcgcgtcgg acctggcctg aaccagacgc cagaaaacga gatggggccg
     4201 ctgatctccg ccgcacaccg cagcaaagtg ttgggctata tcgacagtgg cgaagctcag
     4261 ggggctaacc tgcgtgtgga cgggcgtggc ctcagagtga aagggcacga gcagggctat
     4321 ttcgttgggc cgactctgtt cgataacgtc accccggaga tgaccatcta taaagaagaa
     4381 attttcggac cggtgctttc tgtggtgcgc gtagcagatt acgccagcgc ggtggatctg
     4441 attaaccgac acgaatatgg taatggctcg gccattttca cccgtgacgg tggctgcgca
     4501 cgccgctttt gtgaagaggt gcaggcgggt atggtggggg tcaacgtgcc gatcccggtg
     4561 ccaatggcat tccactgttt cggtggctgg aaacgttcca tttttggcgc gctcaatgtt
     4621 cacgggaacg atggcgtgcg tttctacacc cgaatgaaaa ccatcactgc acgctggccg
     4681 gaaatgcagc aggagacagg tgcagcgttc tccatgccga cgctgggctg acagggggtt
     4741 ttatgcaaac cgaacgtatg actatggccc aggcgctggt cagatttctt aaccaacaat
     4801 atgttagcgt tgatggtaaa gaaacgccct ttgtggaggg cgtggcaacg atctttgggc
     4861 acggtaacgt gctcggtatc ggccaggcgc tggagcagga tcctggtcat ctgcaggtga
     4921 tgcaaggctg taacgagcag gggattgccc atatggcgac gggattcgcc aaacagcatc
     4981 gccgtcagcg tatttttgct gtcacctcat ccgttgggcc tggcgctgcc aatatggtga
     5041 cggcggcagc gacggccacg gcgaaccgca ttccactgct gctactgcca ggggatatct
     5101 acgcctgccg ccagcctgac cccgtgttgc agcagatcga gcagtatcac gatctcagca
     5161 tcagtaccaa cgattgcttc cgcccggttt cccgctactg ggaccgcatc aaccgtcctg
     5221 aacagcttat gagcgcgatg ttaagtgcga tgcgaacgct tactgacccc gcaaatacag
     5281 gagcggtgac catttgccta cctcaggatg tgcaggggga ggcctgggat taccccctga
     5341 gtttctttgc gcgtcgggtg catcatattg aacgccgtcc gcctgacccg cagcgactgg
     5401 cgcaggctca tgcattgatt tcccgcaagc gccgtccact ggtggtgtgc ggcggtggcg
     5461 tgcgttactc cggtgcgcat gaggcgttac gggaatttgt tgaagtgctg caattgccgt
     5521 ttgccgaaac ccaggccggt aagggggcgc tggtaagtga ccacccgctt aatctgggcg
     5581 gggtgggcgt gacgggcgga atggctgcca accagcttgc gccgcaggct gacttagtga
     5641 ttggcatcgg cacgcgcctg acggatttta ctaccggctc taaggcgctc ttttctcatc
     5701 ccgacgtcga gttcctgctg ctgaacgtgg ctgagtttga ctccctgaag ctggatgcca
     5761 ccgcgctcat tgccgatgcc cgaactggtc tgacagcgtt gacccacgca ctgaaagatt
     5821 atcgtagtgg ctggggggag gcgattgccc atgcgaaatc cgcttggcac gatgaatgct
     5881 gtcgactgtg ggaacgccag tggcatcctg acgacgttcc cgaagtggca gggcaccttg
     5941 atgcacagct aatggaatac ggtgaaacgc tcaacacaca cctgacccag acgcgggtgt
     6001 tggggctcat taaccagcat attgaggata acgccattgt ggtgggggcg gccggatcgc
     6061 tgccagggga tttgcaacgc ctctggcagg ttaaaacgcc tgacagttac catctggagt
     6121 atggttactc ctgcatgggg tatgaggtcg cagcggctat tggtgcgaag ctggcaaaac
     6181 ctcagcagcc ggtgtatgcc atggtaggcg atggttcgta catgatgctg cactcagagc
     6241 tgcaaactgc cgtacaggag ggaattaagg tcaccattct gctgtttgat aacgccagct
     6301 tcggctgcat taataacctg cagatggggc acggaatggg gagttttggc acagaaaatc
     6361 gttaccgtaa tccgaaaaca ggtcagcttg acgggccgtt ggtgaaggtc gattttgcgc
     6421 aaaacgcgga aagctacggt tgccgcgcat ggcgggtaca taatgagaaa agcctgctgg
     6481 cggcgctgga agcctcgcgc gctcacccgg gaccgacgct cctcgacatc aaagtgctac
     6541 caaaaaccat gacccatgat tacgcgtcat ggtggcgaac cggcaacgca caggtggcag
     6601 agtcgccagc agtgcgccag gccgccgagc aaacacaggc tcaggtgaaa aaagctcgtc
     6661 agtattgagt tttcctgtcg taacgaaatt ttcatttcat ttttcttttt tcccctcacc
     6721 catcccctct cccaacggga gaggggatgg tacagttttt attccctccc ccccttgtaa
     6781 tggagggaca tatcttcctc ggcgttgtga gattgataac aaaatttatc taatacatct
     6841 caaaagtgtt aactggcact caaaatataa ttttcactat ttataaaaat gaaataaatg
     6901 ttttatttgt agtgtcgtta gttcaccgca ttcatcaaca ccgggagccg ttatgtttaa
     6961 cattgcttta ctgggcgctg gccgaattgg ccaggtacat gcagctaaca tcgccagcca
     7021 tagtgcgacc acgctctggt ctgtggtaga cccgaatcag gagttcgcca cgcgtctcgc
     7081 gacacagtat caggcgcgcc agcagagcct ggatgaggcg atgaccgatc cgaacgttca
     7141 tgccgtattg atcgcctccg cgaccgacac ccacgcggat ctgattgaaa tggccgcccg
     7201 ttacggcaaa gccattttct gtgaaaaacc ggtacacctt gatcttgccc gcgtacgcga
     7261 ctgcctgaaa gtggtcaaag agtatgacgt accgctgttt atcggtttta accgccgctt
     7321 tgacccacag ttccgtcgcg tgaaaaccga cactcaggcc gggcgtatcg gcaaaccgga
     7381 atcactgctg atcatctccc gcgatccgtc tccaccgcct gcggagtacg tgcgtgtttc
     7441 cggcggcatg ttccgtgata tgaccatcca cgactttgac atggcacgct tcatcatggg
     7501 tgaagagccg gtgtcggtgt atgcacaggg cagcaatctg gtggatccgg cgattggcgg
     7561 agcgggagat atcgacaccg catttatcgt tctgaaatat gcctccggcg cgatggcgac
     7621 catcgtcaac agtcgccgct cttcatacgg ctacgaccag cgcctggagc tacacggttc
     7681 cgaagggctg ctgtgcgcgg gcaatatcct tgaaaaccag gtgcagcact acggcaaaca
     7741 gggttgcacc agcgcgctgc cggaacactt cttcctgcaa cgctacaaat ccgcttacgc
     7801 agcggaatgg gagcactttg ttgcggttct gcgcggcgaa gcggtgcctg attgcagcgg
     7861 cgatgatggt gaacgtgcgc tgtacctcgc ggataaagcg ctggaatcac tgcgttgcca
     7921 gcgtgagatc gtcctctaaa cacaacaacc ctacacagag agacataata atgaaaaaac
     7981 tgatcttagc cgccctaatc gccatgacat ccggggccgc catagccgaa aacgagcaaa
     8041 ttgttttcag tacgccaaac ctggccatgc cgtttgaagt tcacatgcag cgcacggcgg
     8101 tgaaagccgc aaaagagatg ggggtgaaac ttcaggtact ggacggtcag ggcagctcac
     8161 cgaagcaggt ggctgacctg gaaaacgcca tcacccgtgg tgcgcagggc tttatcgtct
     8221 ccccgaacga tgtgaacgcg gtctccagtg cggtagatga aattcaggat acgaagctgc
     8281 cggtcgttac gcttgaccgc tctgtcgaca gccagaaaaa agtgccgcac tttggtgcca
     8341 acaactacaa gggcggccag gcgattggcg acttcgtcaa aaccaaattc cccaacggcg
     8401 cggagatcat tctgttgacc ggtcaaccgg gttcctcctc caacattgaa cgcaccaaag
     8461 ggatccgtga cagcctgaag gctggcgggg atatgtacaa gatcgtcgcc gatcagaccg
     8521 gcaactggat gcgctcggaa gggatgcgca ttgtcgaaag cgttttgccg tcgctgccaa
     8581 aacgtcctca ggtgatcctc tctgccaacg atgatatggc gctgggggcg attgaagcgc
     8641 tgcagagtca gggtgtgaaa ccgggtgaaa tcctggtcac cggttttgat gcggtaccgg
     8701 aagcactggc ccgcgtgcgc gacggctggc tggcggttac ggcggatcag cgtcccggct
     8761 ttgcggtgac cacggcgatg agccaactgg tggctaacgt gcgtgagaaa aaaactatca
     8821 ccggggcgga ctatccgccg acgctgatca ccaaagagaa cctgcaacag gctgagcgta
     8881 ttgctgaggc cggtaactga tttgtctgga gccgccgtac gggcggctcc ccataaataa
     8941 ggagcaggct gatgtcgcaa cccttactga aagtaaccga cctggcaaaa agcttttccg
     9001 gcgtatgggc gctgagcagc gcccagctca ccgtcggcac gggtgaaatt cacgctttgc
     9061 tgggcgaaaa cggcgctggg aaatccacgc tgttgaaggc actcgcgggc gcacagccac
     9121 aaacgcgcgg ggagatctgg ttcaatggcg aaacgctgcc ggtggacgac tccccggtag
     9181 aacggcagaa caagggcatt atcaccatct atcaggaatt caacctgctg cccaatatga
     9241 ccgttgcgga aaacatgttt ctcggacgcg aaccgcgtaa acgtaacctg atcgtcgacg
     9301 aaaaagcggt caatcaggaa gcgcaggtga ttctcgatta tctgcaactg aatgtcgcac
     9361 ccaccacgcc agtcgcccgt ctgagcgtgg cccagcagca gatggtggag attgcccggg
     9421 cgctgaccct caatgccagg ctgatcatca tggatgaacc ctccgccgcc ctgagtgaca
     9481 gcgaggtgga aagcctgcac cgcgtggtgc gggagcttaa aaaccgcggc gtgagcatta
     9541 tttacgttac tcaccgtctg catgaagttt tccagctttg cgatcgtttc accgttttcc
     9601 aggatggccg tttcaccggc accggcgcag tgacggagac gaacgtagag cagttgatcc
     9661 gcctgatggt agggcgtgat gtggccttta accgccgccc tgccagcgaa acccaccatg
     9721 aagacaaacc tgtccgtttg tcggtgaagg ggttaagccg ggaaaaaccg ccgctggatc
     9781 cacacggcat cgccctgcat gacatcagtt ttcacgtcca tgctggggaa gttctgggta
     9841 tcgctgggct ggtgggggct ggtcgtactg aggtagcgcg ctgcctgttt ggcgccgacg
     9901 cctttaccac cggcagcttt gaactggacg gcgtgccgta ccagccgcgt gacccacttt
     9961 atgcgctgga gcaagggctg gcgctggtgc cggaagatcg caaaaaagag ggggcggtgc
    10021 tgggtctgtc gattcgcgac aacctgtcgc tctcctgcct ctcctcgctg ttgcagtggc
    10081 gctggtttgt gaatacccgc aaagaggacg acctgattga gtcctaccgc caggcgctgc
    10141 agatcaaaat ggtcaacagc acgcaagagg tgcgtaagct ctctggcggc aaccagcaga
    10201 aagttatcct cgcgcgctgc atggcgctca atccacgggt gttgattgtc gatgagccga
    10261 cgcgtggcat cgacgttgga accaaatcgg aagtgcacca ggtgctgttt gatatggcga
    10321 aaaagggcgt ggcggtgatc gtgatctcct ccgatctgcc tgaagtgatg gcggtgtctg
    10381 accggatcat cacccttagt gaaggccggt taactggcga gatccacggt gacgacgcta
    10441 acgaagaacg gctgatgacg atgatggcta tcaaccacaa cgccctgaac gcggcataac
    10501 ggagtttctc atgacgcaga tgattcctaa aacgcaggtc ccggtaaaac gcgccggacg
    10561 catcgatccc attgccttct tcgagcgctt cggggtactg attttcatga tcctgctgct
    10621 gatcttcttt cagtcgcaga acagtaattt tttctctgaa cgcaatattt tcaacatcct
    10681 gaccgaagtc tccatctacg gaatcatggc cgtggggatg accttcgtca ttctgaccgc
    10741 tgggatcgac ctctccgtgg gctctattct ggcagtgtgc gccatgacag cggcgtacgt
    10801 gatcaaaggc gacaatttca ccacagtcga tccgaacgct tggggcggta tgagctggct
    10861 gattgggctg gggatttgtc tgaccatggg tacagcgatt ggcttcctgc acggtctggg
    10921 cgtcacccgc ctgcgcctac caccgttcat cgtcaccctt ggcgggatga ccatctggcg
    10981 tggtctgacg ctcgtgatta acgacggtgc gccgattgcc ggcttcgatc agggttatcg
    11041 ctggtgggga cgaggggaat tgctcggcat ctccattccg atctggatct tcgccatcgt
    11101 tgccattggt ggttatctgg cactgcataa aacccgttgg gggcgcttcg tctatgcaat
    11161 cggtggcaac ccggaggcgg cacgtctggc tggggtaaac gtgaagcgtg tgctggttag
    11221 cgtctatgtg ctgattggct gtctggcggg gctggcgggc ttcatcctga gcgctcgtct
    11281 ggggagcgct gaggcggttg ccgggatctc ctttgagctg cgtgttattg cctcggtggt
    11341 tattggcggc acgtcgttga tggggggata tgggcgtatc ggcggcacta tcatcggctc
    11401 catcatcatg ggcattctga ttaatggcct ggtactgatg aacgtctcgg cctactacca
    11461 gcaaattatc accggattaa ttatcgttct ggcggtggca ttcgacacct atgccaagag
    11521 ccgtcgcggc gcactgtgag gatcacgatg aaagaggttc gtattggatt gattggcacc
    11581 gggtatattg gcaaggcgca tgccattgcc tatgcccagg caccgacggt gttcaatctg
    11641 cgcggcaaac tggtgcgcga gatggtggct gaggtgaatc cggcgctggc ggcagaacgc
    11701 gcgcaggcgt ttggcttcaa tcgctcaacc ggagactggc gggtgctcgt ggccgacccg
    11761 gccattgatg tggtggatat ttgctcccct aaccatcttc acaaagagat ggcgctggag
    11821 gcgatccgcc acggcaagca cgtctattcg gaaaagccgc tggcactgaa tgcccacgac
    11881 gcgcgtgaga tggtcgacgc tgcgaagcgg gcaggggtga aaacgctggt gggtttcaat
    11941 tacatgaaaa acccgacggc acagttggcg aaagagatta ttgcgcgcgg cgaaattggt
    12001 gaggtgatcc acttctacgg aacccacaac gaagactata tggccgaccc gctttcccct
    12061 attcactggc actgttttaa agagaccgcc ggactggggg ctctgggtga cctggcggca
    12121 catatcgtca atatggccca gtatctggtg ggagagattg agcaggtctg tggcgatctg
    12181 aaaattgtgg tgccggcacg tccggcaaag gccggatcgt cggaaatggt caccgtggaa
    12241 aatgaagatc aggcccacgc gatggtgcgc tttgcgggcg gggcgcaggg cgtcattgaa
    12301 acttcccgcg tcgcctgcgg ccgcaagatg gggctgtcat acgtcattac tggtaccaaa
    12361 ggagcgatca gttttaccca agagcggatg gcagagataa agctctatct gcatgatgat
    12421 ccggtgaacc gtcaggggtt ccagacactg ttggtcggtc cggcacaccc ggattacggc
    12481 gcgttttgca tgggcgcagg tcatggtatc ggctttaacg atcaaaaaac ggtggaagtg
    12541 cgtgatctgg tagacggcat tgccaccgac gcaccgatgt ggccggactt cgaagagggc
    12601 tggaaggtct cgcgcgtgct ggatgccatc gcactctcac accagcaggg cctctggctg
    12661 aacgtgaatg acattgtctg aagagggttc aacgtggaaa agcagtttga tgtgatatgc
    12721 atgggccgcg tggcggtaga cctctacagc cagcaaattg gtgcccgcct ggaggatgtg
    12781 tcgagcttcg ccaaatatct cggcggctcg tccggcaacg tggcgtacgg taccgcgcgt
    12841 cagggcttac gctcgtcaat gctggcgcgc gttggcgatg aacatatggg gcgcttcctg
    12901 cgggaagagt taaaccaggt gggctgcgat accagccatc tgatcaccga taaagaacgc
    12961 ctcacggcgc tggtgctact cggcattaaa gaccgggaca cctttcctct gattttttat
    13021 cgcgataact gcgcggatat ggcgattact gccagcgatg tggacgaaag ctacattgcc
    13081 tccgcacggt gtattgccat caccgggact cacctttctc acccgcagac ccgcgaggcg
    13141 gtgctgacgg cgctgaatta cgcccgccgt cacggcgtgc gcacggtgct ggacatcgac
    13201 taccgtccgg tgctgtgggg actaacctct ctaggagacg gcgaaacgcg ttttatcgtt
    13261 gccgaacagg ttacccgtga acttcaggag gtgctacacc ttttcgacgt cgttgtcggc
    13321 accgaagagg agtttcacat tgcggggggc agcaccgata cgttgcaggc gctggcgcag
    13381 gtgcgccacg tcagcaaggc gacactggtt tgcaagcgcg gcgcgctggg ctgttcggtg
    13441 tataccgatg ccattccacc tcggctggat gacggcctga cggtgaccgg cgtgcgtgtg
    13501 gaggtgctta acgtccttgg cgcgggagat gcgtttatgt ctggcctgct gcgcggttac
    13561 ctgaacgacg agggctggga gcaggcgtgc cgctacgcca acgcctgtgg ggcgctggtg
    13621 gtctcgcgcc acggctgtgc accggcgatg ccgagcaaaa ttgagctgga tgattacctc
    13681 tcccgcgccg cgctcgttcc gcgcccggac attgacccgc gtcttaacca tctgcaccgg
    13741 gtgaccaccc gccgccgcga atggccggag ctgtgcgtga tggcgttcga tcaccgcagt
    13801 caactggaag atatggcgct gcagtgcggc gcgtcgctta agcgtattcc ggcgctgaaa
    13861 cagctgatcc tgcaggccag ccgagaggcg gcaagctctg ccgggctgga ggggaaagcc
    13921 ggcctgctgt gcgatggcac ctttggtcag gatgcgctga acgccattac cggcgaaggg
    13981 tggtggattg gtcgtcccat tgaactgcca ggctcgcgtc cgctggagat ggagcacggt
    14041 aacattggta cccagcttat cagttggccg caggagcatg tggtgaagtg cctggtcttt
    14101 ttccatccgg aagatgccca cggcttgcgt ctggagcagg agcagaaaat cgccgaggtg
    14161 taccacgcct gctgtcagtc cggacatgaa ctgctgctgg aggtgatatt acccgccact
    14221 atgccgcgca gtgacgaact ttatctgcgt gccatttccc gcttctacaa ccttggcatt
    14281 taccccgact ggtggaaact gccgccgctc tccgccgaag gctggacggc actgagcgag
    14341 attatcgcgc gccgggaccc gcactgccgc ggcgtggtga tcctcggtct ggatgctccg
    14401 gcggaacaac tgcgtgccga cttcaaagct gccgcggggc aggcgctggt gaaaggcttc
    14461 gccgtggggc gcacgctgtt tggcgatgcc tcccgcgcat ggctgaaaca cgatattgac
    14521 gatgcgcagc tggtggcgcg catccgggac aactacctgc agcttatcgc ctggtggcgc
    14581 gagcgcggac acgcataatg gagacgacaa tgagtgtaca actgggcatc aacccgctga
    14641 cgtggacgaa cgatgatctg ccttcactgg gcgcggagac atctctggag acctgcctga
    14701 gtgaagggaa agaggccggt tttgctggct tcgaactggg caataaattc ccgcgagaag
    14761 cgcgcctgct cgggcccatt ttgcagcgcc acgacctgca gttggtctcc ggctggtatt
    14821 ccggccgcct gttggaacgc agtgtagagg acgagattgc tgctgtgcag tcgcatctga
    14881 cgctgctgcg agagctgggt gcaaaagtac tggtttttgc ggaagtgagt ggctgcattc
    14941 acggtgagca gcagacgccg gttcacctgc gcccacgttt cccgcaggaa tgctggaaag
    15001 agtatggtga gaagctcacc gtgttcgccc gctatacgca gcaacagggg gtacagattg
    15061 cttaccacca tcatatgggc acggtgattg agtccgctca agacgtggac aacctgatgc
    15121 tccacacggg tgaggaggtt ggtttactgc tggataccgg ccacctgacc tttgccgggg
    15181 ccgatccgct ggcggtggca cagcgatggg catcgcgcat caatcatgtt cactgcaaag
    15241 atgtgcgcgc cgacgtgctg gcggacgtca aaaaccgtaa aaccagcttc ctcgacgcgg
    15301 tgctgagcgg cgtgtttacc gtgccgggtg acggctgcgt ggattatccg ccgattatgc
    15361 aactgctgaa aacgcaggat tatcacggtt ggctggtggt ggaggctgaa caggacccgg
    15421 cgatcgccca tccgctgacc tacgcccgtc tgggatataa caatctgagc cgcctggcgc
    15481 gcgacgccgg gcttatctga ggaggtgaca tgtcacgtct gttatcacgc tggcaacagc
    15541 cgaacgctga ggggcggact cagtctgtca cgccggaaag cgccggatgg ggatatgttg
    15601 gctttgaggc ctatgaactt gaggaagggc agcaactgac gctgcccgct gtcagtgaag
    15661 agcgctgtct ggtgctggtg gccgggcgcg ctttcatcag cactccttct gcacagttta
    15721 ctgatatcgg ggagcggatg agcccgttcg agcgcatcaa accttgggcg gtttacgtca
    15781 ccccgcagga ggcagtgcag gtgaaggcgc tgacgaaact ggagctggca gtctgtaccg
    15841 ctccgggaaa agggacatac ccgacgcggt tgattgcacc tcaggatatc gatggcgaag
    15901 cgcgcggtaa cggccacaac cagcgttatg tgcataatat tttgccggaa gacaagcccg
    15961 ctgacagcct gctggtcgtc gaggtctgga ccaacgaagg ctgtaccagt tcgtacccga
    16021 gccataaaca cgatacgaat aacccgcctc aggagagcta tctggaggag acctattacc
    16081 atcgcctcaa cccggaacag gggttctgca tgcagcgggt gtataccgat gaccgcacac
    16141 ttgatgaatg tatggcggtc tataaccgtg acgtggtcat ggtgccgaaa gggtaccacc
    16201 cggtggcgac gatggccggg tatgacagtt attaccttaa cgtgatggcc ggaccagtac
    16261 gtaaatggat attcacctgg gaaaaggaac actcatggat aaacacagag tattctgcca
    16321 tttctttata aactgagtac ctaagctaaa aaaggggaat gactcccctt ttgtataata
    16381 tgaactcgac aatgttattt caaagtctga gtgatgattt ttctctcagg cagtatgttc
    16441 ctcaataagg atgagaatgt attctgttca ttggcatatg catttatatt ctaatgagct
    16501 gaattttata aaggttgtgg ctattctgga gtataaatat tttttcatta gacatgaaag
    16561 tctatatgat ggtgtattaa tat
//
LOCUS       DAPHDW010000099        16552 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.100, whole genome shotgun sequence.
ACCESSION   DAPHDW010000099 DAPHDW010000000
VERSION     DAPHDW010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16552
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.100"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(119..925)
                     /gene="trpA"
                     /locus_tag="RH057_003151"
     CDS             complement(119..925)
                     /gene="trpA"
                     /locus_tag="RH057_003151"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HDV3129482.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPIEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            complement(925..2118)
                     /gene="trpB"
                     /locus_tag="RH057_003152"
     CDS             complement(925..2118)
                     /gene="trpB"
                     /locus_tag="RH057_003152"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HDV3129483.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            complement(2130..3491)
                     /gene="trpCF"
                     /locus_tag="RH057_003153"
     CDS             complement(2130..3491)
                     /gene="trpCF"
                     /locus_tag="RH057_003153"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HDV3129484.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLP
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDEQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEEQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            complement(3492..5087)
                     /gene="trpD"
                     /locus_tag="RH057_003154"
     CDS             complement(3492..5087)
                     /gene="trpD"
                     /locus_tag="RH057_003154"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HDV3129485.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAD
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGEIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            complement(5087..6649)
                     /gene="trpE"
                     /locus_tag="RH057_003155"
     CDS             complement(5087..6649)
                     /gene="trpE"
                     /locus_tag="RH057_003155"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HDV3129486.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGDRPATLLLESADID
                     SKDDLKSLLLVDSALRITALGDTVTIQALSGNGEALLTLLDNALPAGVENEQLPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYVADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     misc_feature    complement(6671..6764)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            complement(6741..6785)
                     /gene="trpL"
                     /locus_tag="RH057_003156"
     CDS             complement(6741..6785)
                     /gene="trpL"
                     /locus_tag="RH057_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HDV3129487.1"
                     /translation="MKAIFVLKGWWRTS"
     gene            6923..7804
                     /gene="yciV"
                     /locus_tag="RH057_003157"
     CDS             6923..7804
                     /gene="yciV"
                     /locus_tag="RH057_003157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HDV3129488.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQLIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLAALARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            7801..8421
                     /locus_tag="RH057_003158"
     CDS             7801..8421
                     /locus_tag="RH057_003158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HDV3129489.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            8522..9394
                     /gene="rluB"
                     /locus_tag="RH057_003159"
     CDS             8522..9394
                     /gene="rluB"
                     /locus_tag="RH057_003159"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HDV3129490.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGGRRSGGRNNG"
     gene            complement(9434..10024)
                     /gene="cobO"
                     /locus_tag="RH057_003160"
     CDS             complement(9434..10024)
                     /gene="cobO"
                     /locus_tag="RH057_003160"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HDV3129491.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIKHAFDAGVKAQIGIDY"
     gene            complement(10021..10779)
                     /locus_tag="RH057_003161"
     CDS             complement(10021..10779)
                     /locus_tag="RH057_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HDV3129492.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSEDCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDICPMSEQNPQVWQDVMQVNVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            10999..12048
                     /gene="sohB"
                     /locus_tag="RH057_003162"
     CDS             10999..12048
                     /gene="sohB"
                     /locus_tag="RH057_003162"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HDV3129493.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            complement(12084..12335)
                     /locus_tag="RH057_003163"
     CDS             complement(12084..12335)
                     /locus_tag="RH057_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HDV3129494.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            12715..15312
                     /gene="topA"
                     /locus_tag="RH057_003164"
     CDS             12715..15312
                     /gene="topA"
                     /locus_tag="RH057_003164"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HDV3129495.1"
                     /translation="MGKALVIVESPAKAKTINKYLGSDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALALQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNKYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVNKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            15522..16496
                     /gene="cysB"
                     /locus_tag="RH057_003165"
     CDS             15522..16496
                     /gene="cysB"
                     /locus_tag="RH057_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HDV3129496.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGSPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
BASE COUNT         4038 a         4199 c         4399 g         3916 t
ORIGIN      
        1 ttgacgttcg accggggtaa gcgaaacggt aaaaagataa atattaaatg aatttaggat
       61 ttttccggct tcattaaaga aagttaaaat gccgccagcg gaactggcgg ctgtgggatt
      121 aactgcgcgt cgccgctttc atcggttgta caaaaacttt cagtgccgcc agcattttct
      181 ctggctcatt aatatgttgc tcgatgattt taacaatggc cgaaccagaa atcgcgcccg
      241 cagctcctgc atcaatcgct gcttttacct gatccggggc ggaaatacca aacccctgca
      301 atggaggtgc agcgttgtac tctttcagct tcgcaaccag atgattgagg ggtaacgcgg
      361 cgcggttttc tgcgccggtc acgcccgctc gtgacagcaa ataggtgtaa ccacgaccgt
      421 aagaggctat ctggcgcagc aggtcgtcat cggcatttgg cgggcagatg aagataggtg
      481 cgacattatg gcgcaacgcg gcctggcgga agggcgcgga ctcttcaatt ggcacatcgg
      541 caaccagcac cgaatcgacg ccgactttct cgcactgggc ataaaactca tcaatgcctt
      601 tgttaaacac cagattggca tacatcaaca ggccaatggg aatggtcggg tgtttctggc
      661 gaatcagtgc cagcatttca aaacattgtg ccggagtcac acctgccgca aaggcgcgca
      721 gagtggcgtt ttgaatggtc gggccatccg ccagtgggtc ggagaagggg atacctaact
      781 ccagcgcgtc agcaccggct tcaattagcg tatcgataat tttcaatgac tgctcaatgc
      841 ccggatcacc gagcgtgacg aaaggaacga atgcgccttc tttgcgctcc ttcaactggg
      901 caaacagaga ttcgtagcgt tccatcagat ttcccctcgt gctttcaaaa tatcgtgaac
      961 ggtgaagatg tctttatcgc cgcgaccgga aaggttaacc accagtagct gctctttatc
     1021 cgggttttcg cgcatcattt tcaacgcatg ggccagggcg tgggaggatt ccagcgccgg
     1081 gatgatccct tcgtgcaggc acagcgtttt gaaggcttca agggcttcat catcggtaat
     1141 agacacgtaa tcagcgcgtc cagtgctgtt aagatacgcg tgttgtgggc cgacagacgg
     1201 gaaatccagt ccggcggaga tggagtaaga ttcttcaatc tgcccgtctt cggtttgcat
     1261 catcggcgct ttcataccga aatagatacc cacgcgacca tgttttagcg gtgcgccgtg
     1321 ctcgccagtt tcgataccgt gaccacctgg ctccacacca atcaggccga cgttggtttc
     1381 attgatgaaa tcagcaaaca tgccgatggc attcgaaccg ccgccaacac aggcgataac
     1441 ggcatccggc aggcgacctt ctctttccag aatctgcgct ttggtttctt cgccaatcat
     1501 ccgctgaaac tcacgcacaa tggtcggata aggatgcggg ccagctgcgg tgcccagcat
     1561 atagtgcgcg gtttcgtaac taccggacca gtcgcgcagc gcctcgttac aggcatcttt
     1621 cagcgtcgcg gaaccgctat gcaccgggat cacttccgca cccattaagc gcatacgaaa
     1681 aacgttaggc gactggcgtt caacgtcttt ggcacccata taaatacggc atttcaggcc
     1741 gagcagggcg ctggcaaggg ccgacgccac gccatgctga ccggcaccgg tttcggcgat
     1801 gatttcggtt ttacccatcc gcttcgccag caacgcctgc cccagcacct ggttagtttt
     1861 atgcgcgccg ccgtgcagca aatcttcacg cttgagatac agcgtggtgt tcgtcccggc
     1921 tgtaatgttc tggcatttgg tcagcgcggt tggacgcccg gcatagtttt tcagcaggtc
     1981 gttgaactga gcctgaaatt caggatcttt ttgcgcactg acaaaagctt cttccagctg
     2041 gcgcagagca ggcatcagga tttgtggcac gtacatgccg ccaaactcac caaaataggg
     2101 gttaagtaat gttgtcattg ttcctttcct taatatgcgc gcagcgtctg gaaaaccgag
     2161 gccaaaagac gtgcgtcttt gatgcccggt tgcgactcta cagcagaatt aaaatcaagt
     2221 ccggcgcagc cggtttgtgc cgcttccacg cagttatctg cgcctaagcc ccccgccagc
     2281 agaacgttgc caagcgattg accatttaat agtgaccagt caaaacgttg cccgcttcca
     2341 ccctggccgt tgtctaaaac atatttatca acgtgctgaa actcgcgggc gggcagggtt
     2401 tcaccgacgc ttaatgcttt ccagatggca acatgtgctg gcagagcttc acgcagcgta
     2461 tcgatataca gctgttcttc attaccatgc agttgcactg ccgccagcga taacacctta
     2521 gctttgtcca ccacatcggc aatatcgtga ttgcggaaca cgccaacata ctgcaacggt
     2581 gccgcagcca tcacttcctg cgcctgttca acgttgacgc aacgcggtga tgtcgcaaca
     2641 aaaatcaacc caccgtaaat cgcgcccgcg tcataagctg ctttagcatc ttgcccacgc
     2701 gtcaggccac atactttatt ctcacccagc aacacccggc gcacggcggc gtgcaaatcg
     2761 tcatgggcca tcaacgccga accaatcaga aaaccgttag cgaagtggct taactcgcgc
     2821 acctgagcgt aagtattgat gccggattcg ctgattaccg tcacgttgtg ccccagtttc
     2881 ggcgcaagct cgcgggtacg gttgagatca atcgacaaat cacgcagatc gcggttgttg
     2941 atgccaacga cctttgcccc caatgcaatg gcacgctcca gttcctcttc attactgact
     3001 tcggtcagca cacccatctc cagactgtgg gcgacggcgg caagctggcg atattgttcg
     3061 tcatccagta ctgaaagcat taataagcag gcatcagcct ggtaatagcg cgccagatag
     3121 atctggtaag gatcgataat gaagtcttta cataaaatcg gctgcggggc gatttggctg
     3181 acgatgggga ggaaatcaaa gctcccctga aaatatttct catcagtcag caccgaaatt
     3241 gccgaagcgt aatgtttata aatggcggca atgcgtgctg gatcgaaatc atcacggatc
     3301 acgccttttg acggcgacgc tttcttgcac tccagaataa acgccgtgcg cgcaccctgt
     3361 agcgcatcat aaaaatgtcg cgtgctcggc tgaacctcat tctgaaaact ggccagcggt
     3421 tgctgctgtt tgcgggtttc tacccaaatc gccttgtctg cgacgatttt cgctaaaacg
     3481 gtttgcatca tttaccctcg tgccgccagt gcggtaactc tgtcgtaagc ggaaccactg
     3541 cgcagtacct caagaacggt ttgcgcattg gcttgcagat cttcatggcc atgcaggcgc
     3601 attaacatgg cgacgttcgc agcgacggct gcttcatggg ctgcgtcgcc tttaccttgt
     3661 aacaagcgtg ttaaaatgtc acggttttct tccggtgttc cgcctgccag ttgctcctgg
     3721 tggtagggag tcaggccaaa atcttcagcg gtcaattgat agctcttaat ttcgccgtca
     3781 tgcagctcgg caacgattgt cggcgcgtgt aatgaaactt catccatccc gccgctgtgc
     3841 accaccgccg cgcgttgata ccccagcacg cgcaaggttt cggcaatcgg cagcaccagt
     3901 tccggactat aaacaccaat taacgccagc ggcggatgcg ccgggttaat caatggcccc
     3961 agcacattga acagggtgcg ggttttcagt tgctggcgaa ccggcatcgc gtggcggaat
     4021 ccggtgtgat acttcggcgc aaagaggaaa catacaccta actcatccag cgcctggcgc
     4081 gatttatcgg cgttcatatc aagattaata ccgaacgccg ccagcagatc ggacgaacca
     4141 gatttactgg agacgctacg gttgccgtgt ttcgccactt tcagcccaca ggccgcggcg
     4201 acaaacgcac tggcggtaga aatattgata ctgttgctgc cgtcaccgcc agtaccgacg
     4261 atatcagcaa acagataatc cgggcgcggg aacggcgcgg cgttttccag tagcgcggtt
     4321 gctgctccgg cgatctcgtt cgggtgctca ccgcgaattt tcatgctcac cagcgccgcc
     4381 gccagttgtt ccggcttcag ctcgccacgc accaccgctg aaaacagctg gtggctttct
     4441 tgttggctaa gcgtctgcgc ctgatacagt ttttccagaa tcggttgcag cgtgttggct
     4501 ggctctagtt tctgctgcgc ccaggccagc gtttgttcca gcaggcgagc gccctgggtg
     4561 gtgagaatgg attccggatg gaactggaat ccacaaacgc gatccgcatc gtgacgtact
     4621 gccatcacca tgccattaaa atgggcgttg atggttaaac cggccggaat gttactgcca
     4681 accagcgagt gataacgcgc caccggcagc gggtttgtta atccggcaaa catcgcctga
     4741 ccgtcatgtt caatgctcga cgctttaccg tgaagaattt cgcccgcctg accgacatag
     4801 cccccgtaag cttcgacaat tgcctgatgt ccgaggcaaa tgccaataat gggcagcttg
     4861 ccacgcaagc gggtgaggag ttccggcata caaccggctt cgctcggcac accggggcca
     4921 ggagaaagca tcagtaccgg attgctcatc gtcgccaggc gttcaattaa ggtctgcgcc
     4981 ggaatatggt tgcggtaaat caccacatta tgcccattgc tgcgcaactg atctgccagg
     5041 ttgtacgtaa aagagtcgat attatcgagc agcagaatgt cagccatcag aaagtctcct
     5101 gtgcatgatg cgcggtggca atagcgcgca gtacagcgcg ggctttatta cgggtttcgt
     5161 cggcttccga ctgcggaaca gaatcaagga ctacgccagc accggcttgc acggtggcga
     5221 taccgttttc caccagcgcc gagcggatca caatgcaggt gtcgagatcg ccatgcgcgg
     5281 tgaaataacc taccgcgccg ccgtagctgc cgcggcggcg accttccgcc tcggcaatta
     5341 actgcatagc gcgtactttc ggcgcaccac ttaacgttcc catattcata caggcgcggt
     5401 aagcgtgcag ggcgtcgaga tcgtggcgca gttcaccgac cacgcgggag accaggtgca
     5461 tcacgtaaga gtaacggtca actttggtga ggtcggcgac gtagcggctg ccgggggtgc
     5521 aaatgcgtgc cagatcatta cgggcgagat ccaccagcat cagatgttca gaaagctctt
     5581 tatgatcggt acgcatttcc agttcgatgc ggctgtcgag gtctctgtcc agcgagccat
     5641 cggcacgacg accgcgtgga cgtgttccgg caatcgggta gatctcaatc tggcggctgg
     5701 tggcgtcata cttgagcgaa ctttccggcg acgcgccaaa cagggtgaaa tcattatcct
     5761 gcataaaaaa catgtacggg ctgggattac tctttttcag cacgtaatag gccgccagcg
     5821 gtgacgggca gggcagagag aaacggcgag atggcaccac ctggaaaatt tctccagcgc
     5881 gaatcgcttt ttgcaacaaa cgcactacgc caccgaactc ttcatcgctc tgattacatt
     5941 cacaacgcat atgcggcacg gaaaccactg gcagcggcgg cgcggcttcg gtcagttgct
     6001 gacgtagttc gttcaggcga gcagtgagac gttgtttttc ttcttcattc ggagcaaaca
     6061 ggctggcctg aatgcgggtg ctttttttct gatggtcaat caccatcaga gtttcagcga
     6121 gataaaaaca gaaatcaggg cagttatttt ccgctgacag ttgcggtaaa tcttcaaatc
     6181 ccgccacaag gtcataagag aacaggccgc cgaagaacat tgcttctcgt tcttccttcg
     6241 gtacattcaa cagattctgc aataaacgga aagcgtcaaa aaccgaaagg gagcataagc
     6301 gagcgtcttc atccagcagt ggactgacag gggggaagcg cagcacacgg cagtttggta
     6361 attgttcatt ttccacaccc gcaggcaggg cgttatccag tagtgtcagc agggcttcgc
     6421 cgttgccgga aagtgcctgg attgtgacag tgtcacctaa agctgtaatg cgcagcgcac
     6481 tgtctaccag cagcaggctt tttaaatcat ctttgctgtc gatatctgcg gattccagca
     6541 gcagcgttgc cggacgatcc ccacacaact ggtgaaaaag cgcggtggga ttgtcgcgat
     6601 aagcgccttc gcaggttagc agttcgagag tcggtttttg tgtttgcatt gttattctct
     6661 aattttgttc aaaaaaaagc ccgctcatta ggcgggctgg gtatctgatt gctttacgca
     6721 tggtgaatac actgcccgtt tcaggaagtg cgccaccaac ctttcagtac gaaaattgct
     6781 ttcattgtcg ataccctttt tacgtgaact tgcgtactag ttaactagtt cgatgattaa
     6841 ttgtcaacag ctcatttcag aatatttgcc agaaccgtta tgatgtcggc gcaaaaaaca
     6901 ttatccagaa cgggagtgcg ccttgagcga cacgaattat gcagtgattt acgacctgca
     6961 cagccatacc acagcttccg atggctgcct gacgccagaa gcattggtgc accgtgcagt
     7021 cgagatgcgc gtcggcaccc tggcgatcac cgaccatgac accacagctg caatcgcgcc
     7081 agcaagagag gaaatttcac gttcgggact ggcgttgaat cttattcccg gcgtggaaat
     7141 ttccacggtc tgggaaaatc atgaaattca tattgtcggg ctgaatattg atattactca
     7201 tccgctaatg tgtgagttcc tcgcgcagca gacagaacgg cgcaatcagc gggcgcagct
     7261 gattgccgaa agacttgaaa aagcgcaaat acctggggcg ctggaaggcg cacaacgact
     7321 ggcgcagggc ggcgcagtga cgcgcggtca ttttgcgcgt ttccttgttg agtgcggcaa
     7381 agccagttca atggcggatg tctttaaaaa gtatctggcg cgcgggaaaa ccggatacgt
     7441 tccgccacag tggtgtacaa tagaacaagc tattgatgtc attcatcatt ctggcggtaa
     7501 ggcggtgcta gctcatcctg ggcggtacaa tcttagtgct aaatggctga aaagattggt
     7561 tgcgcatttt gccgaacacc acggtgacgc gatggaagtc gcgcagtgcc agcaatcgcc
     7621 caatgaacgt acccagctgg cggcccttgc gcgtcagcat catttatggg catcacaagg
     7681 atctgatttt catcagccat gcccgtggat cgaactgggt cgtaaactct ggctgcccgc
     7741 aggcgttgaa ggcgtctggc agctatggga acagccgcag aacaccacag agagggaatt
     7801 atgagccagt ttttttatat tcatcctgat aacccacagc aacgtctgat caaccaggcg
     7861 gtggagatcg tgcgtaaagg cggggtgatt gtttatccaa ctgattccgg ctatgcgctc
     7921 ggctgtaaaa ttgaagataa aaacgcgatg gagcgtattt gtcgtattcg ccagctgccg
     7981 gacggtcaca actttaccct gatgtgtcgc gatctttctg aactctcgac ctattcattt
     8041 gttgataacg ttgcgtttcg tttaatgaaa aacaacacgc cgggcaacta taccttcatc
     8101 ttgaagggga cgaaggaagt gccacgccgt ctgttgcagg aaaaacgcaa aaccatcggt
     8161 atgcgtgtgc cttctaaccc gattgcgcag gcgttgcttg aagcactggg cgaaccgatg
     8221 ctttccactt cgctaatgct gccaggcagc gaatttaccg aatccgatcc cgaagaaatt
     8281 aaagaccgtc tggaaaaaca ggtggatttg attatccacg gcggctatct cggacagaaa
     8341 ccgacaacgg ttattgatct taccgatgat acgccggtag tggtgcgtga aggcgtaggt
     8401 gatgtgaagc ctttcttata agtgcgtaac tctgtatact acgcggcctt aaaatggcgc
     8461 tatacggcgc cattgattct cctgttcgac gcctgtgaag gcgacaccaa aaggaagctc
     8521 tatgagcgaa aaattacaga aagtgctggc ccgcgctggc cacggctccc gccgtgaaat
     8581 cgaatctatt attgaagccg gtcgtgtgag tgttgatggc aaaattgcta aactcggcga
     8641 tcgcgttgaa gttacccctg ggctgaaaat tcgtatcgat ggtcatctga tttcggtacg
     8701 tgagtccgct gaacaaattt gtcgcgttct ggcctattac aaaccggaag gtgagttgtg
     8761 tacccgtaac gatccggaag ggcgtccgac ggtgtttgac cgtctgccaa agctgcgtgg
     8821 cgcacgctgg attgccgtag gtcgtctgga cgttaacacc tgtggtctgc tgctgttcac
     8881 caccgatggt gaactggcaa accgtttaat gcatccaagc cgtgaagttg aacgtgaata
     8941 cgccgtgcgt gtatttggtc aggttgacga cgcgaaactg cgtgatttga gtcgtggcgt
     9001 gcaactggaa gatggtccgg cagcttttaa aaccatcaag ttcagcggcg gcgaagggat
     9061 caaccagtgg tacaacgtga ctctgaccga aggccgtaac cgtgaagttc gtcgtctgtg
     9121 ggaagcggtt ggtgtgcagg tgagccgcct gatccgtgtt cgttacggtg atatcccact
     9181 gccaaaaggt ctgccacgcg gtggctggac ggagctggac ctcgcccaga ctaactatct
     9241 gcgcgaactg gtggagcttc cgccagaaac cagctctaaa gtcgctgtag aaaaagaccg
     9301 tcgtcgcatg aaggctaatc agattcgtcg tgcggtgaaa cgtcacagtc aggtgagcgg
     9361 cggtcgtcgt tctggcgggc gtaataacgg ttaatcaaag tatgccggac gtcatatccg
     9421 gcatttttac agattaataa tcaatcccta tttgcgcctt aacaccggca tcaaacgcat
     9481 gtttgatggg gcgcagctca cttaccgtat ccgccagttc aagaatatcc cgatgacagc
     9541 ctcgacccgt gataatcacc gtctgttgat gtggacgttc atttaacgcc tgcaccactt
     9601 cttccagtgg caaatagtca tacgccacca tatacgtcag ttcatcaagc aaaaccatat
     9661 ccagtgagga atcagcaagc atccgctttg catgttgcca gacttcgcgg caggcggcgg
     9721 tatcagactc gcggttttgt gtatcccagg taaagcccgt tgccatcacc tgaaactcaa
     9781 cgccatgtgg ctccagcaga ttgcgttcgc cattaggcca ggtgccttta ataaactgca
     9841 cgacgcccac tttttttccg tgaccaactg cgcgtgttgc cgtaccaaat gccgcggtgg
     9901 ttttgccttt tccattgccg gtaaagacga tgataatacc gcgttcatcc tgggcctggg
     9961 ccacacgagc atctactttt tctttcactc gctgctgacg ctgttggtag cgttcatcac
    10021 tcattgggaa attcctggtt tacggcccgg ttgggcgtca aaggtcatgc cggttttacg
    10081 gcggctgtca tcgcccatca gccagaggta gagcggcatg atatcagcgg gtgttttaag
    10141 tttctgtgga tcttcggtcg ggaaggcgct tgcgcgcatt gcggtgcgcg taccgcctgg
    10201 gttaatgcag ttgacgcgca ggcgctgctg atattcatcg gccagtacct gcatcatccc
    10261 ttcggtggca aatttcgacg ctgcatatgc accccagtta gctcgtccct gacgtccaac
    10321 gcttgatgaa gtaaagacca gtgaaccggc gtccgatttg agtaataaag gaagcagtgc
    10381 ctgggtgagc ataaaggtgg cattaacgtt gacctgcatg acgtcctgcc agacctgcgg
    10441 attttgttcg ctcattgggc aaatatcgcc gagcaatccg gcattatgca aaacaccatc
    10501 cagacgcgga taattaacga caatgcgctg tgccagttgt tggcaatctt cggacgtgca
    10561 ggtcagcaaa tcgagaataa accactgtgg ctgacgtcca gtttcttcgt ttatgtggct
    10621 ggctacctga cgtaattttt cttcattacg gcccaacaga atcactgtcg caccatagcg
    10681 tgcatacgtc atcgcggctt cacggccaat cccatcgctg gctcccgtca ccaggataat
    10741 gcgatcattg agtaaatctt gttttggctg gtaatgcatg gctactcctc aacgacgttg
    10801 tctgtcgcgc tgccagtgta ctttatgact ttggggcttt atgcctgaaa cagaagggca
    10861 tttcaatcag cctggggcga taacaccgca aaatggatac tttgtcatac tttcgctgca
    10921 ataacatctc tgcgagacgg cttaacatgc ctgttgtaaa ctgtgagcca aagcgttgtt
    10981 taaccaaggt ggggactcgt ggaattgttg tctgaatatg gtttgttttt ggcgaaaatc
    11041 gttaccgttg tgctagcgat tgcggcgatt gccgccatta ttgtcaatgt tgctcaacgt
    11101 aataaacgcc agcgtggcga gttacgggtc aacaatctca gcgaacagta taaggagatg
    11161 aaagaagaac tggccgcggc gctgatggac tcacatcagc aaaaacagtg gcacaaagcg
    11221 cagaagaaaa agcacaagca agaagcgaaa gcagcaaaag cgaaagccaa actgggcgag
    11281 gtggcaactg acagtaaacc tcgcgtctgg gtgctggatt ttaaaggcag catggacgcc
    11341 catgaagtga actcgctacg tgaagagata acggctgtac tcgcagcatt caaaccgcag
    11401 gatcaggttg tgctacgtct ggaaagccct ggtggcatgg tgcatggtta cgggttggcg
    11461 gcttcgcagc tgcagcgtct gcgtgataaa aacattcctt taactgttac ggtagacaaa
    11521 gtcgctgcca gcggcggtta catgatggcc tgtgtggcgg acaaaattgt ttccgcaccg
    11581 tttgctattg tgggttccat tggggtggtg gcgcaaatgc ccaactttaa ccgcttcctg
    11641 aaaagcaaag atattgatat cgaactgcac actgccgggc agtataagcg tactctgacg
    11701 ttgctgggtg aaaataccga agaagggcgg gagaaattcc gcgaagagtt gaacgaaacg
    11761 catcagttgt ttaaagattt tgtgaagcgt atgcgtccgt ctctggatat tgaacaggtg
    11821 gcaacgggtg aacactggta cggacaacag gcggtagaga aaggcctggt tgatgaaatc
    11881 aacaccagtg atgaagttat tcttagcctg atggaaggcc gtgaagtggt taatgtacgc
    11941 tatatgcagc gtaaacgact cattgaccga ttcaccggca gcgcggcaga gagcgccgat
    12001 cgattgttgc tacgctggtg gcagcggggt caaaagccat tgatgtaaaa gacaaacgcg
    12061 aggctaagac ctcgcgtttt gctttaatca accagatgat atttttctga aagcacatgg
    12121 gccaggtgtt tgaacatatt aaacaccgcg gtgcttttgg ctgttggcaa tccttgttca
    12181 tctaaaaagt agtcgccggt aaataccagc acgccattac gctgcgtgac gccggtggct
    12241 tcaatccctg cgagcgtatc ctcatgctca cgaatgattt tgttggcctc tttcaacagc
    12301 gtttcgcggt cgatgggttg tgtctctttg ttcattattt actccttaaa caaggacatt
    12361 agtctacgcc aggcatggct tgcagacaaa tataccacgc tggtggcaag agcgccttac
    12421 tggcaacttt ggattttgca tgctaataaa gttgcgtatc ggattttatc aggtacagtg
    12481 tgacgctttc gtcaatctgg caatagattt gcttgacatt cgaccaaaat tccgtcgtgc
    12541 tatagcgcct gtaggccaag acctgttaac tcagtcacct gaattttcgt gaacagagtc
    12601 acgacaaggg gttgatatcc gcagagaacg agtccatatc ggtaactcgt tgccagtgga
    12661 aggtttatca acgtgcgacg cattcctgga agaatcaaat taggtaaggt gaatatgggt
    12721 aaagctcttg tcatcgttga gtccccggca aaagccaaaa cgatcaacaa gtatctgggt
    12781 agtgactacg tggtgaaatc cagcgtcggt cacatccgcg atttgccgac cagtggctca
    12841 gctgccaaaa agagtgccga ctctacctcc accaagacgg ctaaaaagcc taaaaaggat
    12901 gaacgtggcg ctctcgtcaa ccgtatgggg gttgacccgt ggcacaattg ggaggcgcac
    12961 tatgaagtgt tgcctggtaa agagaaggtc gtctctgaac tgaaacaact ggctgaaaaa
    13021 gccgaccaca tctatctcgc aaccgacctt gaccgcgaag gggaagccat tgcatggcac
    13081 ctgcgggaag tgattggggg tgatgatgcg cgctatagcc gagtggtgtt taacgaaatt
    13141 actaaaaacg cgatccgcca ggcatttaat aaaccgggtg agctgaatat tgatcgtgtt
    13201 aatgcccagc aggcgcgtcg ctttatggac cgcgtggtgg ggtatatggt ttcgccgctg
    13261 ctatggaaaa agatcgctcg tggtctgtct gccggtcgtg tgcagtcggt ggcggttcgc
    13321 ctggtggtcg agcgtgagcg tgaaattaaa gcgttcgtgc cggaagagtt ctgggaagtc
    13381 gatgccagca cgaccacgcc atctggtgaa gcgttggcgt tacaggtgac tcatcagaac
    13441 gacaaaccgt tccgtccggt caacaaagaa caaactcagg ctgcggtaag tctgctggaa
    13501 aaagcgcgct acagcgtgct ggaacgtgaa gacaaaccga caaccagtaa acctggcgct
    13561 ccttttatta cctctacgct gcaacaagct gccagcaccc gtcttggatt tggcgtgaaa
    13621 aaaaccatga tgatggcgca gcgtttgtat gaagcaggct atatcactta catgcgtacc
    13681 gactccacta acctgagcca ggacgcggta aatatggttc gcggttatat cagcgataat
    13741 tttggtaaga aatatctgcc ggaaagtccg aataagtacg ccagcaaaga aaactcacag
    13801 gaagcgcacg aagcgattcg tccttctgac gtcaatgtga tggcggaatc gctgaaggat
    13861 atggaagcag atgcgcagaa actgtaccag ttaatctggc gtcagttcgt agcctgccag
    13921 atgaccccag cgaaatatga ctccacgacg ctgaccgttg gtgcgggcga tttccgcctg
    13981 aaagcacgcg gtcgtatttt gcgttttgat ggctggacaa aagtgatgcc tgcgttgcgt
    14041 aaaggcgatg aagatcgcat cttaccagca gttaataaag gcgatgctct gacgctcgtt
    14101 gaacttacac cagcccagca ctttaccaag ccgccagccc gtttcagtga agcatcgctg
    14161 gttaaagagc tggaaaaacg tggtatcggt cgtccgtcta cctatgcgtc gatcatttcg
    14221 accattcagg atcgtggcta cgtgcgagta gaaaatcgtc gtttctatgc ggaaaaaatg
    14281 ggcgaaatcg tcaccgatcg ccttgaagaa aatttccgcg agttaatgaa ctacgacttt
    14341 accgcgcaga tggaaaacag cctcgaccag gtggcaaatc acgaagcaga gtggaaagct
    14401 gtactggatc acttcttctc ggatttcacc cagcagttag ataaagctga aaaagatccg
    14461 gaagagggtg gtatgcgccc gaaccagatg gttctgacca gcattgactg cccgacttgt
    14521 ggtcgcaaaa tggggattcg cacagcgagc accggggtat tccttggctg ttctggttat
    14581 gcgctgccgc cgaaagagcg ttgcaaaacc accattaacc tggtgccgga aaacgaagtg
    14641 ctgaacgtac tggaaggcga agacgctgaa accaacgcgc tgcgcgcaaa acgtcgttgc
    14701 ccgaaatgcg gcacggcgat ggacagctat ctcatcgatc cgaaacgtaa gttgcatgtc
    14761 tgtggtaata acccaacctg cgacggttac gagatcgaag agggcgaatt ccgcattaaa
    14821 ggttatgacg gcccgatcgt tgagtgtgaa aaatgtggct ctgaaatgca cctgaaaatg
    14881 gggcgtttcg gtaaatacat ggcctgcacc aacgaagagt gtaaaaacac acgtaagatt
    14941 ttacgtaacg gcgaagtggc accaccgaaa gaagatccgg tgccattacc tgagctgccg
    15001 tgcgaaaaat cagatgctta tttcgtgctg cgtgacggtg ctgccggtgt gttcctggct
    15061 gccaacactt tcccgaaatc gcgtgaaacg cgtgcgccac tggtggaaga gctttatcgc
    15121 ttccgcgacc gtctgccgga aaaactgcgt tatctggccg atgcgccaca gcaggatccg
    15181 gaaggtaata agaccatggt tcgctttagc cgtaaaacca aacagcaata tgtctcttcg
    15241 gaaaaagacg gaaaggcgac tggctggtca gcattttatg ttgatggcaa atgggttgaa
    15301 ggaaaaaaat aacctttaat tctgtcaggt ttttataaac aaagggtcgc gaaagcggcc
    15361 cttttttatt gcatattatt ttttcttcac acctatacac taaggctata aatgatatag
    15421 tggttatagt tagcaccttt tttattatta aatcgtatta gtcacccgcc aggtgtgacg
    15481 aaaaaacgat gttctgatgg cgtctaagtg gatggtttaa catgaaatta caacaacttc
    15541 gctatattgt tgaggtggtc aatcataacc tgaatgtctc atcaacagcg gaaggacttt
    15601 acacatcaca acccgggatc agtaaacaag tcagaatgct ggaagacgag ctaggcattc
    15661 aaattttttc ccgaagcggc aagcacctga cgcaggtaac gccagcagga caagaaataa
    15721 ttcgtatcgc tcgcgaagtc ctgtcgaaag tcgatgccat aaaatcggtt gccggtgagc
    15781 acacctggcc ggataaaggc tcgctgtata tcgccaccac gcatacccag gcacgctacg
    15841 cgttaccaaa cgttatcaaa ggctttattg agcgttatcc ccgcgtttct ttgcatatgc
    15901 accagggctc gccgacacaa attgctgatg ccgtctctaa aggcaatgct gatttcgcta
    15961 tcgccacaga agcgctgcat ctgtatgaag atttagtgat gttaccgtgc taccactgga
    16021 atcgggctat tgtagtcact ccggatcacc cgctggcagg caaaaaagcc attaccattg
    16081 aagaactggc gcaatatccg ttggtgacat ataccttcgg ctttaccgga cgttcagaac
    16141 tggatactgc ctttaaccgc gcagggttaa cgccgcgtat cgtcttcacg gcaacggatg
    16201 ctgacgtcat taaaacttac gtccggttag ggctgggggt aggggtcatt gccagcatgg
    16261 cggtggatcc ggtcgccgat cccgaccttg tgcgtgttga tgctcacgat atcttcagcc
    16321 acagtacaac caaaattggt tttcgccgta gtactttctt gcgcagttat atgtatgatt
    16381 tcattcagcg ttttgcaccg catttaacgc gtgatgtcgt tgatgcggct gtcgcattgc
    16441 gctctaatga agaaattgag gtcatgttta aagatataaa actgccggaa aaataatttc
    16501 ttgcgttatt ttcggcacct tttatgtagc gaaggtgccg gaatatattc tc
//
LOCUS       DAPHDW010000100        16521 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.101, whole genome shotgun sequence.
ACCESSION   DAPHDW010000100 DAPHDW010000000
VERSION     DAPHDW010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16521
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.101"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(72..224)
                     /locus_tag="RH057_003166"
     CDS             complement(72..224)
                     /locus_tag="RH057_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HDV3129497.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            complement(489..1223)
                     /gene="cysH"
                     /locus_tag="RH057_003167"
     CDS             complement(489..1223)
                     /gene="cysH"
                     /locus_tag="RH057_003167"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV3129498.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMSEEETRFFGLKRECGLHEG"
     gene            complement(1297..3009)
                     /gene="cysI"
                     /locus_tag="RH057_003168"
     CDS             complement(1297..3009)
                     /gene="cysI"
                     /locus_tag="RH057_003168"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HDV3129499.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMAKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            complement(3009..4808)
                     /gene="cysJ"
                     /locus_tag="RH057_003169"
     CDS             complement(3009..4808)
                     /gene="cysJ"
                     /locus_tag="RH057_003169"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HDV3129500.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPAALAATPAPAAEMPGITIISASQTGNARRVAEALRDDLLTAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDADVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTPIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGELRVFIEHNDNFRLPAN
                     PETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            5127..5489
                     /gene="queD"
                     /locus_tag="RH057_003170"
     CDS             5127..5489
                     /gene="queD"
                     /locus_tag="RH057_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HDV3129501.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            5567..6838
                     /locus_tag="RH057_003171"
     CDS             5567..6838
                     /locus_tag="RH057_003171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HDV3129502.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARAGLSVLLLERAEIPGSKNL
                     SGGRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGVTTFSSLQPGGESWSVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGRICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRPLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGWYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWRHLRRHGLWHLAGDVIRSLRCL"
     gene            6829..7089
                     /locus_tag="RH057_003172"
     CDS             6829..7089
                     /locus_tag="RH057_003172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HDV3129503.1"
                     /translation="MSVAHNLWRVADAPHIVPADSVERQMVQRLINACPAGLFSLTPE
                     GDLRVDYHGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            7106..7681
                     /locus_tag="RH057_003173"
     CDS             7106..7681
                     /locus_tag="RH057_003173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HDV3129504.1"
                     /translation="MPLLHLLRQNPVIAAVKDNASLQLAIDSECQFISVLYGNICTIS
                     NIVKKIKNAGKYAFIHVDLLEGASNKEVVIQFLKLVTEADGIISTKASMLKAARAEGF
                     FCIHRLFIVDSISFHNIDKQVAQSNPDCIEILPGCMPKVLGWVTEKIRQPLIAGGLVC
                     DEEDARNAINAGVVALSTTNTGVWTLAKKLL"
     gene            complement(7829..8689)
                     /locus_tag="RH057_003174"
     CDS             complement(7829..8689)
                     /locus_tag="RH057_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HDV3129505.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCILAHWQIEPQPVVAEQV
                     LDALVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSVRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     TRDPLAEARRVLVVGQGGEADNQEIAMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFVVAINHDASAAVFSQADVGVVDDWKVVLEA
                     LVTNIHADCQ"
     gene            complement(8686..9465)
                     /locus_tag="RH057_003175"
     CDS             complement(8686..9465)
                     /locus_tag="RH057_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HDV3129506.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGKSGPDISLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQHQNPLDLIITGCQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            complement(9443..10852)
                     /locus_tag="RH057_003176"
     CDS             complement(9443..10852)
                     /locus_tag="RH057_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3129507.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     AALTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLRQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            complement(10874..12328)
                     /locus_tag="RH057_003177"
     CDS             complement(10874..12328)
                     /locus_tag="RH057_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HDV3129508.1"
                     /translation="MSLSRAAIVDQLKEIVGADRVITDETVLKKNSIDRFRKFPDIHG
                     IYTLPIPAAVVKLGSTEQVSRVLNFMNAHKINGVPRTGASATEGGLETVVENSVVLDG
                     SAMNQIINIDIENMQATAQCGVPLEVLENALREKGYTTGHSPQSKPLAQMGGLVATRS
                     IGQFSTLYGAIEDMVVGLEAVLADGTVTRIKNVPRRAAGPDIRHIIIGNEGALCYITE
                     VTVKIFKFTPENNLFYGYILEDMKTGFNILREVMVEGYRPSIARLYDAEDGTQHFTHF
                     ADGKCVLIFMAEGNPRIAKATGEGIAEIVARYPQCQRVDSKLIETWFNNLNWGPDKVA
                     AERVQILKTGNMGFTTEVSGCWSCIHEIYESVINRIRTEFPHADDITMLGGHSSHSYQ
                     NGTNMYFVYDYNVVDCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            complement(12398..13183)
                     /locus_tag="RH057_003178"
     CDS             complement(12398..13183)
                     /locus_tag="RH057_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417254.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HDV3129509.1"
                     /translation="MSIESLNAFSMDFFSLKGKTAIVTGGNSGLGQAFAMALAKAGAN
                     IFIPSFVKDNGETKEMIEKQGVEVDFMQVDITAEGAPQKIIAACCERFGTVDILVNNA
                     GICKLNKVLDFGRADWDPMIDVNLTAAFELSYEAAKIMIPQKSGKIINICSLFSYLGG
                     QWSPAYSATKHALAGFTKAYCDELGQYNIQVNGIAPGYYATDITLATRSNPETNQRVL
                     DHIPANRWGDTQDLMGAAVFLASPASNYVNGHLLVVDGGYLVR"
     gene            13502..14779
                     /locus_tag="RH057_003179"
     CDS             13502..14779
                     /locus_tag="RH057_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3129510.1"
                     /translation="MQHNSYRRWITLAIISFSGGVSFDLAYLRYIYQIPMAKFMGFSN
                     TEIGLIMSTFGIAAIILYAPSGVIADKFSHRKMITSAMIITGLLGLLMATYPPLWVML
                     CIQVAFAITTILMLWSVSIKAASLLGDHSEQGKIMGWMEGLRGVGVMSLAVFTMWVFS
                     RFAPDDSASLKTVIIIYSVVYILLGILCWFFVSDNNNLRSANNEEKQSFQLSDILAVL
                     RISTTWYCSMVIFSVFTIYAILSYSTNYLTEMYGMSLVAASYMGIVINKIFRALCGPL
                     GGIITTYSKVKSPTRVIQILSVLGLLALTALLVTNSNPQSVAMGIGLILLLGFTCYAS
                     RGLYWACPGEARTPSYIMGTTVGICSVIGFLPDVFVYPIIGHWQDTLPAAEAYRNMWL
                     MGMAALAMVIVFTFLLFQKIRTADSAPAMASSK"
     gene            14806..16284
                     /locus_tag="RH057_003180"
     CDS             14806..16284
                     /locus_tag="RH057_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HDV3129511.1"
                     /translation="MSKKYIIGIDGGSQSTKVVMYDLEGNVVCEGKGLLQPMHTPDAD
                     TAEHPDDDLWASLCFAGHDLMSQFAGNKEDIVGIGLGSIRCCRALLKADGTPAAPLIS
                     WQDARVTRPYEHTNPDVAYVTSFSGYLTHRLTGEFKDNIANYFGQWPVDYKSWAWSED
                     AAVMDKFNIPRHMLFDVQMPGTVLGHITPQAALATHFPAGLPVVCTTSDKPVEALGAG
                     LLDDETAVISLGTYIALMMNGKALPKDPVAYWPIMSSIPQTLLYEGYGIRKGMWTVSW
                     LRDMLGESLIQDARAQDLSPEDLLNKKASSVPPGCHGLMTVLDWLTNPWEPYKRGIMI
                     GFDSSMDYAWIYRSILESVALTLKNNYDNMCNEMNHFAKHVIITGGGSNSDLFMQIFA
                     DVFNLPARRNAINGCASLGAAINTAVGLGLYPDYATAVDKMVRVKDIFIPIESNAKRY
                     DAMNKGIFKDLTKHTDVILKKSYEVMHGELGNVDSIQSWSNA"
BASE COUNT         3930 a         4356 c         4170 g         4065 t
ORIGIN      
        1 aattttaaaa aagggtgcag gagaccaaac catcacaatt cagtacgcgg ggttttaccc
       61 ccgcgtggtg gctacttctt cgattcgtaa gcgagaactg ccttaaactc cataccacgt
      121 tctctgatac tcaactcaca caacgagtca cctaccatca gcgtgaatcc cagtactgta
      181 caacacagta cgatgattgc cacaagggca tattttgtca gcatgtcttg cctccctgca
      241 aagaagagac taacattcga attgtttggg ttcgaacgta agcctcaggt tgatagaaat
      301 atcgcctggg gcttttgtcc atctggaacc tcgcgaatgc ttaacgccag acagcctcaa
      361 gcacccgacg ccattctata cctgataatt cttccagcgg cgttcacgtt taaaatcagg
      421 aatactgctc gcaaaataaa aacgccccca tcatttctga cagaggcgtt taatttatcc
      481 ggcaatattt acccttcgtg caatccacat tcccttttta agccaaagaa acgtgtttct
      541 tcttccgaca tgccaggttc ccatttacgg gttgtatggg tatcaccgac cgataaatat
      601 ccttcatccc ataatgggtg atatttcagg ccatgttttt gcaggtactg ataaatagtt
      661 cggttatccc agtcgataat cggcagcact ttaaatacgc cacgctgaat tgccagcacc
      721 ggtaaattgg cgcggctgcc ggattgttcg cggcgcaggc cagcaaacca cgtttgcgca
      781 ttcagttctt tcagcgcccg gttcatcggt tcgactttgt tgatgtcatt gtacttttca
      841 atgccttcaa cgccctgttc ccacagtttg ccgtagcgtg cttcctgcca ggcggcgctt
      901 tcggtagcgc ggtacacttt caggttgagc ttgagtttgt ccgttaactc gtcaataaag
      961 cggtaggttt ccgggaacag atagccggtg tcggtgagga tcaccggaat atccgggtga
     1021 atctgattca ccagatgcag gctcaccgcc gcctgaatgc caaagctgga agaaagtaca
     1081 tattcaccgg gcagattatc cagcgcccag gcaacgcggc cttcagcgtc cagtttttcc
     1141 agttcggcgt tagtttctgc cagcgccaga atgcgatcta ccttcggcag ttcgttcagg
     1201 gcgtttagat cgagtttgga catagattcc tcactgtttg ccttgcctga tgcgacgctc
     1261 acgcgtctta tcaggcctac aagaccgggc tgatggttaa tcccacaaat cgcgcgccgg
     1321 atcgagcacc gggcgaatga tgcccgcacg caccgtaaag tcgccgaagc cttcacccgc
     1381 ttcgcgctct ttcgcccagc gccctatcag ttcatcaagc gacgccagga tttccggctc
     1441 ggtgatgttt tctttataca tccgtgggat acgtgtccca atgcggttgc cgccaagatg
     1501 cagattgtag cgacccggtg ctttacccac caggcccact tccgccagca tcgcgcgacc
     1561 acaaccgttc gggcagcctg ttacacgcat cacgatatgc tcatcgctga caccatgttt
     1621 cgccattaaa ttatcgatgt tgtcgataaa agacggcagg aaacgctctg cttccgccat
     1681 cgccagcggg caagtcggga atgacacgca ggccatcgag ttttcacgct gcggcgtgac
     1741 ggcattcatt aacccgctct ctttggcgat cttctcgatc ttcgctttct cgctttccgg
     1801 tacaccggcg atgatcagat tctggttcgc cgtaatgcgg aaatcgcctt tgtggatctt
     1861 cgcgatctcc agcaggccgg ttttcagcgg acgccccgga taatcaagga tgcgaccatt
     1921 ttcgataaac agcgtcaggt gccagttatc atcaatgccc ttaacccagc caatacgatc
     1981 gcctcgtccg gtgaactcat atggacggat cggttcaaat ttgatccccg cgcgacgctc
     2041 cacttccgct ttaaacgtct caacccccac gcgctccagc gtgtatttgg ttttggcatt
     2101 tttacgatcg gttcggttac cccagtcacg ctgagttgtc acgacggctt cggccaccgc
     2161 cagcgtatgc tccagcggca gatagccaaa ctcgctcgcc gtgcgggcgt aggttttctt
     2221 gttgccgtgt tcgatagaaa gcccaccgcc caccagcagg ttgaagccta ccagcttgcc
     2281 gttttcggcg attgccacga agttcatgtc gttggcatgc agatcgatat cgttctgcgg
     2341 cgggatcact accgtggttt tgaacttacg cggcaggtag gtctgaccaa ggatcggttc
     2401 ttcatcagta gtggcgactt tttcctggtc gagccagatc tccgcatacg cgcgggtacg
     2461 aggcaacaga tgctcagaaa tcttcttcgc ccactcgtac gcttccgcgt gcagctgcga
     2521 ctcgtaaggg ttcgaggtgc agagtacgtt acggttcatg tcgttagctg tcgccagcgc
     2581 atcaagaccg accgagtgca gcatctggtg caccggtttg acgttctttt tcagaatgcc
     2641 gtggaactga aacgtctggc ggttggtcag gcgaatgctg ccatagatgg tgttttcacc
     2701 ggcaaattta tcgatcgctt gccactgttt ggtggtgatc acgccacccg gcagacgaca
     2761 gcgcaacagc atcgcatggc gcggctccag cttctgttca gcacgttcgg cgcggatgtc
     2821 gcggtcatcc tgctgataca tgccgtggaa gcggatcagc aggaagttat cgcccttaaa
     2881 gccgccggtc agaccgtcgt ttaaatcttc cgcaatggtg ccgcgcaggt agttgctttc
     2941 aagcttcatg cgctcggcgt ctgtcagttt accttcgacc actaaaggcc ctggatgttt
     3001 ttcgctcatt agtagacatc tcgctgataa cggcgctcta cgcgcagctc acttaaaaat
     3061 tcatccgccg cttcggtgtc catgccacca aattcggcaa tcacttccag aagtgcctgc
     3121 tcaacgtctt tcgccatgcg attagcgtca ccgcagacat aaatatgggc accgtcattg
     3181 atccagcgcc acagctccgc gccctgttcg cgcagtttgt cttgtacgta aactttttct
     3241 ttttggtcgc gcgaccaggc gagatcgatc cgcgtcagca caccctcttt gacgtagcgc
     3301 tgccactcca cctggtagag gaaatcttcc gtaaagtgcg ggttgccaaa gaacagccag
     3361 tttttacccg gtgcttcgtc ggcagcacgt tgctgcataa aggcgcggaa cggcgcgatg
     3421 ccggtgcctg ggccaatcat aatcaccggg gtttccgggt tagcgggcag gcggaagtta
     3481 tcgttatgtt cgataaatac gcggagttcg ccctcttctt ccacgcggtc cgcgaggaag
     3541 ctggaggcac caccggcacg ggcgcggcct tccacgtcgt aacgcaccac accaacggtg
     3601 acgtgtactt cgttctcgac ttccgcctgc gaggaggcga tggaatacag acgcggcgtc
     3661 agcgggcgca gcagattaat tagcgcttcg gcatcaagct gtgccgggga gaaacgcacc
     3721 atgtcaacaa tcggcgtcgt cgcggcgtaa tgctgtaact tcgctttatc gcccaccagc
     3781 ggcagcagtg tttcactgcg ggtaagcgtg gcgtaattct caacaatgtt ggcggtgttg
     3841 acggtcagtt cgaagtgcca ctgtagcgct tcgttcagag gcaacgtttt gccctcgacg
     3901 gtgacaggtt catcgcctgt cagccacagc agttcgacaa gttctttcac cagtgccgga
     3961 tcgttctgat accagacgcc cagcgcgtca cccggctggt aacgcaggcc cgagtcacct
     4021 aagtcaattt cgatatggcg aacgtctttt tcagagttac gcccggtaat tttctggtta
     4081 acagagaggc tagccaccag tggcgcgtct ttgctgtacg ggctggtgtg gatttcattt
     4141 accgcgccag tagcgacgga ttgcgaaggt gccgcgacag gcgcacgcga tttaagcgca
     4201 tcaaccacgc gggcgcgcca ctcgctggca gcagcctggt attcaacatc ggcatcgaca
     4261 cggtcgagca ggcgttcacc gcccagttcc gccagcttgc tgtcgaaatc tttcccggac
     4321 tggcagaaaa attcataaga gctatcgccg aggctaaaca cggcaaacgc ggtgttttcc
     4381 agctttggcg cttttttgga gaacaggaac ttatgcagcg cgacggcttc ttccggcggt
     4441 tccccttccc cttgcgttga cgtcaccacg ataagcagtt tttcgctggc gatttgtttg
     4501 aatttatagt cgcccgcgtt caccagctta acgttcagtt ttgccgtcaa taaatcatcg
     4561 cgtaatgctt cagcaacccg gcgcgcattg ccggtttgcg aagcagagat aatagttata
     4621 cccggcattt ctgcggctgg cgctggcgtc gctgcaagcg cagcaggctg ctgattgagc
     4681 acgccccaga aatagccaga aacccaggca agctgggtgg gagttaaatc ggtcgtggcc
     4741 gcctgaaggc gtgccagttg ctccgggttc aacggaagca acgcggaagg tgggacctgt
     4801 gtcgtcatgc gtcgttatgt tccagtaagc aaagctgttt ctgcgccctg tcagcgccca
     4861 taaaacagaa gagaaggtaa ggttaacggg gcaaatggtg tggattaaag acgggatagc
     4921 gataactaat aaccaaatcg actaacctgt tttagcaata gtctttaaca acaaaataga
     4981 ttaaccaacc caatgaaaaa caaatgaatt tagccaatca ttaagataaa tcagcgattt
     5041 tgcgcaacaa agtcgtttta gataatgcga aaaaacagcc tttccggtac tctacggcgg
     5101 ttttatcgtc ctgtagagaa attatgatgt ccaccacgtt atttaaagat ttcaccttcg
     5161 aagccgctca ccgcttacca cacgtcccgg aagggcataa atgtggtcgc ctgcacgggc
     5221 attcctttat ggtgcgactg gaaattaccg gggaagtcga tccgcatacg ggctggatta
     5281 tcgatttcgc tgaactaaaa gcggcgttta aaccaaccta cgagcgcctc gatcaccatt
     5341 atctcaatga tattccaggt ctggaaaacc caaccagcga ggttttagca aaatggattt
     5401 gggatcaggt taaacccgtt gtgccgctgt taagtgcggt gatggtaaaa gaaacctgta
     5461 ccgcaggttg tatctatcgc ggcgaatgat aagagtgtgt cggcggtcaa tttcccttaa
     5521 gtaacgctat gttagggtgt tgtgttctgg atatctgggg catgacatgg aagacgactg
     5581 cgacattatt attattggtg ccggtattgc aggcaccgct tgcgcgttac gctgcgcgcg
     5641 agcgggttta tccgttttgt tactggaacg cgctgaaatc cccggcagca aaaatctttc
     5701 cggcgggcgg ttatataccc atgcactcgc ggaactcctc ccgcaatttc atctgaccgc
     5761 gcctcttgaa cgacgcatca ctcacgaaag cctttccctg ttaacgcccg atggcgtaac
     5821 gacgttttcc agcttacagc ccggcggtga atcctggagt gtattacgtg cacggttcga
     5881 tccgtggctg gttgccgaag ccgaaaaaga aggtgtcgaa tgcatccccg gagcgacggt
     5941 ggatgcactg tatgaagaaa acggcagggt gtgtggtgtc atttgtggtg acgatattct
     6001 ccgcgcccgt tatgtggtgc tggcagaagg tgccaacagc gtcctggctg aacgtcacgg
     6061 attagtgact cgtcctgctg gcgaagcgat ggcgttgggg atcaaagaag tgctgtcgct
     6121 ggaaacatcc gctattgaag aacgttttca tctggagaat aacgaaggcg cagcgttgct
     6181 gttcagcggc aggatctgtg atgacttacc cggcggcgca tttctttata ctaatcaaca
     6241 aacgctctcg ttagggattg tttgcccgct ctcttccctt acgcaaagtc gtgttccggc
     6301 aagcgagctg ctgactcgct ttaaagcgca tccggcagtg cgcccgctta tcaaaaacac
     6361 ggaatcactg gagtatggtg cgcatctggt gccagaaggt ggcttgcaca gtatgccggt
     6421 gcaatacgcc ggtaacggct ggctgctggt gggcgatgcg ttgcgcagtt gcgtcaatac
     6481 cggaatttcc gtgcgcggca tggatatggc gctgactggc gcgcaggcgg cggcacaaac
     6541 gctgataagc gcctgccagc accgcgagcc gcaaaatctg tttgcgcttt atcatcacaa
     6601 cgtagagcgc agcctgctat gggatgttct acaacgttat cagcatgttc cggcgctttt
     6661 gcaacgccct ggctggtatc gggcgtggcc tgcgttaatg caggatattt cccgcgattt
     6721 atgggatcag ggtgataaac ctgttccacc gctgcgccag ttattctggc gtcatttacg
     6781 tcgtcatggc ctgtggcatc tggcgggcga tgttatcagg agtctgcgat gtctgtagcc
     6841 cataatctct ggcgcgttgc tgatgcgccg cacattgttc cggctgactc cgttgagcgt
     6901 cagatggtac aacggttgat taacgcctgt ccggcaggtc ttttttcgct cacaccggaa
     6961 ggtgacttac gcgttgatta tcacggctgc ctggagtgtg gcacctgccg tttgctgtgc
     7021 gacgaatcca cactacaaca gtggcgctat ccgccttccg gattcggcat cacctaccgc
     7081 tttggataaa taataaggat aatttatgcc cctcttacac ctgctccgcc agaatccggt
     7141 gattgctgcc gttaaagata atgccagcct gcaactggca atcgactctg aatgccagtt
     7201 tatttccgtg ttgtacggca atatctgcac catcagtaat atcgttaaaa agattaagaa
     7261 cgccgggaaa tatgctttta ttcatgttga tttgctggaa ggcgcgtcaa ataaagaagt
     7321 cgtgattcag tttttgaaac tggtgaccga ggcggacggc attatcagta ccaaagcctc
     7381 aatgttgaaa gcggcaagag cggaaggttt tttctgtatt catcgcctgt ttattgttga
     7441 ttcaatttcg tttcacaaca ttgataagca agttgcgcaa tcgaatccgg attgtattga
     7501 gatcctgcca ggctgtatgc ccaaagtgct gggctgggtg acagagaaaa tccgccaacc
     7561 gctgattgcc ggtgggctgg tgtgcgatga agaagatgcg cgtaatgcga ttaacgcggg
     7621 tgtcgtggcg ctttccacca cgaataccgg ggtctggacg ttagcgaaaa aattactttg
     7681 acgggataat cgtaaccaat tgaatttggt ttgatttgta ggccgcacgc cacatccgac
     7741 attcagcgcc tgatgcgacg cttgacgcgt cttatcaggc ctacaagtcc ccaggccaga
     7801 accgtaggca ggatcatccg gcaacacgtt attggcaatc tgcgtgaatg tttgtcacca
     7861 gtgcctccag caccactttc cagtcatcaa ccacgccaac atctgcctgt gaaaataccg
     7921 cagcgctggc gtcatgatta atcgccacca caaacttgct attgcgtacg cccgccatta
     7981 acgctgctgc gccagaagcg ccaacaacaa tacacacctc aggtgccagc agatgtccgg
     8041 aaataccaat cactttttca gcatcaacgc caccgttcat cactcgcgcc cgactgtagc
     8101 ccacttcggc tcccagcttt tctgccagca tagcgatttc ctggttatct gcctctccgc
     8161 cctgccccac caccagcacc cgtctggcct cagctaatgg gtcgcgagta acatttttca
     8221 gatcctctgt gctaaccagc cagtcgggaa gcgcacccgg gacaatgttc agttgttgca
     8281 ttccagaggg caacgtggcg tttttcgctg ctcccgcctg acgcgccagg gaaaggcata
     8341 gcgggcgttt ttctgtttgc agcgtcgccg ttagcgcatt tccccagtgg gatttgcgca
     8401 cgcttacggt tggaatatct aacgaagtca cctggcagat gcttgcacca tgtaaacgcc
     8461 aggccagccg ggtcgacagt tcatcgccaa acgttcccgg cggaaagaga acaacgtccg
     8521 caggcgttcg ctgccactgt tccaccaacg catccagcac ctgttccgcc actacaggct
     8581 gaggttcaat ctgccagtgc gccaggatac aaccgctgaa atcctgcgcc gccagccagc
     8641 ttgctattgc ggcattctcc tgatttattg tcacaattgc gatattcatg gctgcatcct
     8701 ttggcgtaaa tagtcctgcc atagcttttg agctttttct gctaccgttt gcccatcaat
     8761 cagcgtcgct ccgcgtcgtt gctctgctct tgccagttgc agacactgca tcgctggcat
     8821 ttcggcagca acagttttac ggataatttc tgctttcccc gcagccattc gctggcgcat
     8881 tcccggtact ggcaaggcca cttcaccgca ctgacgcacg gcaatcaccg caggcaagcg
     8941 aacccggcaa caacgcaaac catgttcagt acgctgttcg agggtgataa acagcgcgtc
     9001 gagagtgaaa cgctccacct gggtgaagca gggccagccc agcatttccg ccagcaaaaa
     9061 tggcgtttgc ccattctgcc cttcgctgct ttggcagccg gtaattatca gatccagcgg
     9121 attctgatgc tgccattcgg caatatggcg agcgacaaat tccggcgcaa agcgcagatc
     9181 tgccgccgtc tccagcaata cagcttcctc aaacccaaga gccatgagat agcgcagcca
     9241 gtgcaatgcc cgttcatccc ccatgctcaa cgcggttaaa gacatcggcg taccgttttt
     9301 tctctgcgcc aacagcagcg cggcagcggc ctgttcatca gcaccgagta aacttcgcag
     9361 tagcgaaata tccggtccgc ttttaccctg agccgccgcc tgccactctt tttccgccag
     9421 cattccggca tccggttcgg ctttaaacgc taacagaatg ttcattcgca cctcctacat
     9481 ttcccgccgc caccagcggg agggctttag tttctggtgc ccatagccag gtcacaacaa
     9541 aaccaaccaa caacactgtc gccaggagca ataaggtgac ttgcattccc cactgcgcca
     9601 gcacccacgg cagcaggcca gtacttaccg ccgcgccaag tcgactcatg gcggtggcaa
     9661 aaccgacgcc cagcgagcga atgtcagtag gaaaactttc cgcaggcaaa atgcccacca
     9721 gattactgac tgccgaaatg gtggtgctga agagaacaaa aagcagcagc gttaatgaac
     9781 tgccggaagg caaacaggcc atgactacca gcgttgccgc cagcagcaaa aaacttccca
     9841 gcaaaaattt gcgatgtgcc agcaggtgcg tcagaactaa tcccagcagc gcgcccacaa
     9901 ttaacaacgc attaagcatc aggctggcag tcagcgcatc ttccagacca atagtctggg
     9961 cgatagttgg cagccaggta taaatcacaa accatgggat tacgaggcag acaaagaaga
    10021 cgctgttaaa cgccgttcgc cgccagtaac gcgaagagaa caaggttttg atgtgtttat
    10081 gggtcgccgt taccacttca tcgcccagta aaacatgggg accaaaatag cgatgcacga
    10141 tagcgtgagc ttctgcaaaa cgcccctggc gcagtagcca gcgtggtgat tctggcgttc
    10201 cccagcgtaa taacgtaatc aacaacgcgg gcagagctgc cgatgccagt agccagcgcc
    10261 aggcctccgg gttttcggaa ataaagtgat gtccggcaat acttgccagc acatagccta
    10321 cggtccacac cacgctgaat gcgcccagca aaataccgcg atggcggcgc ggggaaaatt
    10381 cagccagcaa ggtgtgacct actgaataat cgcctcccag accaatgcca atcaaaatgc
    10441 gcagtccaat aagatgctct ggcgtggtgg caaaaaattg taaaaacgaa gcaagcgtaa
    10501 tcagcaaaaa gctgaaggtg aagatttttt gccgaccaat atggtcggag atccacccaa
    10561 gaaccaggct gccaaggaac aaaccaagga gcgccgagcc gccgatcatt cccgccataa
    10621 acggcgtcag ttgcatggcg ggcgtaagct gaataatggc gtaaccaatg acgccgagaa
    10681 cataaccgtc ggtcaggtgt gcgccgaaag tgagcgcagc aatgcggcag tgaaaacggt
    10741 taagcggtaa atcatccatt cgcaccggtg aagtgttcat ctttacccct tcattgtccg
    10801 gtaataagaa cggcttctcg cctgagaagc cgaaaaggca acgccccgtc atcgcggaac
    10861 gctgcctgac acattatttt tcaatcggat agatagtacc cgtattcata atgccattag
    10921 gatcgaactg ctttttcagc ccttccagca acgcccacgc gctgccgtgt tccagtttgc
    10981 tccagtgaac gcgatgttta ccgataccgt ggtggtgcac catcgaaccg ccgaggcgaa
    11041 tggtttcttc acagatgatc ttgttgagcg gattgtggta cttgtcgatt tcctcttccg
    11101 gcttacagtc aacgacgttg taatcgtaga cgaagtacat gttggtgccg ttctgatagc
    11161 tatgagagga atgaccgccc agcatggtga tgtcgtcggc gtgcgggaac tcagtacgaa
    11221 tacggttaat aacgctttcg tagatttcgt ggatgcagct ccagcagccg gacacttcag
    11281 tggtaaagcc catgttgccg gttttgagga tctgcacacg ttcggcagcc actttatccg
    11341 gtccccagtt caggttgtta aaccaggttt cgatcagctt gctgtccacg cgctggcatt
    11401 gcgggtagcg ggcaacgatt tccgcaatcc cttcgcccgt cgccttcgca atgcgagggt
    11461 taccttcagc cataaagatc agcacgcatt ttccgtctgc aaaatgggtg aagtgttggg
    11521 tgccatcttc agcgtcatac aaacgagcga tcgacggacg atacccttcc accatgactt
    11581 cacgcaggat gttgaagccg gttttcatgt cttccaggat atagccgtag aagaggttgt
    11641 tttccggggt gaatttaaag attttcactg ttacttcagt gatatagcaa agtgcacctt
    11701 cgttaccgat gatgatgtgg cgaatgtccg ggccagccgc gcggcgtggc acgtttttaa
    11761 tgcgtgtgac ggtgccatca gccaatactg cttccagacc aacgaccata tcttcgattg
    11821 cgccgtagag tgtggagaac tgcccgatac tgcgggttgc taccaggccg cccatctgag
    11881 ccagcggctt tgactgcgga gaatgccccg tggtgtaacc tttttcacgc aacgcatttt
    11941 ccagcacctc cagcggaacg ccacattgcg ccgtcgcctg catattctca atatcaatat
    12001 taatgatttg attcatggcg gagccgtcga gcaccaccga gttttccaca acggtttcca
    12061 gcccaccttc agtggcggaa gcgccggtac gcggcacacc gttaattttg tgcgcattca
    12121 taaaattcag cacacgggag acttgttctg tggaaccgag ttttacgacc gctgccggaa
    12181 tcggcaaagt ataaatacca tgaatatccg gaaatttacg aaaacggtca atactgttct
    12241 tctttaatac tgtttcatcg gtaattacgc gatctgcacc aacaatttcc tttagctggt
    12301 cgacaatcgc tgcgcgagat aaagacatag taattccttc ctgataataa aaatggaata
    12361 atcattaata agagaaaaca gaaaccaccg ttatggatta gcgcactaaa taaccaccat
    12421 cgaccactaa taaatgccca ttgacataat ttgaagccgg acttgcgagg aatacagctg
    12481 cgcccattaa atcctgagta tcgccccaac ggtttgccgg aatatgatca agaacgcgct
    12541 gattggtttc tggattactg cgtgtcgcca gcgtaatatc tgttgcataa tagccagggg
    12601 cgataccatt tacctgaata ttatattgac ctagctcatc acaataagct ttggtgaacc
    12661 cggcaagagc atgtttagtg gcagaatatg caggtgacca ttgtccacct aagtaagaga
    12721 acaatgaaca gatattaata attttgccgc ttttctgcgg gatcataatt tttgcagctt
    12781 catagcttaa ctcgaatgcg gcggtcaggt tcacatcaat catcggatcc cagtcggcac
    12841 gaccgaagtc cagcacctta ttcagcttac aaataccggc attgtttacc agaatatcaa
    12901 ctgtaccgaa acgctcacag caagcagcga taatcttctg cggcgcgcct tctgcggtga
    12961 tatccacctg catgaagtcc acctcaacac cctgtttttc aatcatttcc tttgtttcgc
    13021 cgttatcttt gacgaaacta ggaataaaga tatttgcgcc agctttggcc aacgccatgg
    13081 caaatgcctg gcctaaaccg ctattcccac cggtaacaat tgcggtttta cctttcagtg
    13141 agaaaaaatc cattgagaac gcattgagag attcgattga catagttagc tccaatttcc
    13201 gtaaggcaaa aaaaaagaaa ggtaacttcc cccgcgagag ggaagttacc tttctcatta
    13261 tctctggtaa ctggagataa tttttagcat aacggcataa ctgctaatgt gatcgtaatc
    13321 acagtgtgat attcgtattg tcaaatagtt ttaaaaagca gaagcgtgat tactctcaca
    13381 caacacctct gctttccatg ttatttctta ttgcaattac tagagacctg agaaaagtaa
    13441 tccacctgaa agggcggctt acttttctct tttttttgtc tattacaaat gggaatacga
    13501 tatgcaacac aactcatatc gccgttggat aaccctcgcg ataattagtt ttagcggcgg
    13561 cgttagtttc gacctggctt atttacgtta tatttatcaa attcccatgg cgaaatttat
    13621 gggattcagc aataccgaga taggtttaat aatgagtacc tttggtattg cggctattat
    13681 tctttatgcc cccagcggcg ttattgccga taaattttca caccgcaaaa tgattacttc
    13741 cgcgatgatc attaccggat tactgggtct gttaatggca acgtatccac cgctgtgggt
    13801 aatgctctgt attcaggtcg cctttgcgat aacgacgatt ttaatgctgt ggtcggtgtc
    13861 gattaaagcc gcctcgttgc tgggcgatca tagtgagcaa gggaaaatta tgggctggat
    13921 ggaagggctg cgcggcgtcg gtgtaatgtc gctggcggtg tttaccatgt gggtcttttc
    13981 tcgctttgca ccggatgaca gcgccagcct gaaaacagtc attatcatct acagtgtggt
    14041 ttacatcttg ttggggattc tgtgctggtt ttttgttagc gataacaaca acctgcgcag
    14101 tgccaataac gaagaaaaac agtcattcca gcttagcgac atccttgccg ttttgcgtat
    14161 cagcaccacc tggtattgca gcatggtgat ttttagtgtc ttcacaatct acgccattct
    14221 gagttactcc accaactatc tgaccgaaat gtacggcatg tcgctggtgg cagcgagcta
    14281 catggggatt gtgatcaaca aaatcttccg cgcgctgtgc ggcccacttg gcggcattat
    14341 caccacctac agtaaagtga aatcccctac ccgcgtgatc caaatccttt ccgtactcgg
    14401 cctgctggcg ttaactgccc tgctcgtcac gaactctaac ccgcaatcgg tcgcgatggg
    14461 gattggcctg attttactgc tgggattcac ctgttacgcc tcacgcgggc tgtactgggc
    14521 ctgccctggc gaagcgagaa caccgtctta cattatgggc accacggtag gtatttgttc
    14581 ggtgattgga ttcctgccgg atgtcttcgt ttacccgatt atcggccact ggcaagacac
    14641 cctgcccgca gcagaagcct accgcaatat gtggctgatg ggaatggcgg cgcttgccat
    14701 ggtgattgtc tttacctttt tgctgttcca aaaaattcgt actgctgata gcgcccccgc
    14761 aatggctagc agcaagtaag ccaggaaaat tacaggagaa aaatcatgtc gaaaaaatac
    14821 atcataggga ttgatggcgg aagtcagagc acaaaagtgg tgatgtacga tctggaaggt
    14881 aacgtggttt gcgaaggcaa aggcttatta cagccgatgc acacgccaga tgccgatact
    14941 gcagaacatc ctgacgacga tttatgggca tcattatgtt ttgcaggtca cgatttgatg
    15001 agccagtttg ccgggaataa agaagatatt gttggcatcg gcctgggctc catccgttgc
    15061 tgccgtgcgt tattgaaagc cgacggcacg ccagctgcgc cgttgattag ctggcaggat
    15121 gcacgcgtta cacgccctta cgaacatacg aatcctgacg tggcgtatgt cacctctttt
    15181 tcgggttatc tgacgcatcg cttaaccggc gagtttaaag acaatatcgc caactatttt
    15241 ggtcagtggc cggtggatta taagagctgg gcatggagcg aagatgctgc ggtaatggat
    15301 aagtttaata tcccccgtca tatgctgttt gatgtgcaaa tgcctggcac cgtcctcgga
    15361 catatcacac cacaagccgc actggcgaca catttcccgg caggactgcc ggttgtttgt
    15421 accaccagtg ataaaccggt agaagctctg ggggccggat tactggatga tgaaactgcg
    15481 gtaatttctc ttggcactta catcgcattg atgatgaacg gcaaagcact gccgaaagat
    15541 ccggtggcgt actggccgat tatgtcttct attccgcaaa cattgctgta tgaaggttac
    15601 ggtattcgca aaggtatgtg gacggtgagc tggctgcgtg acatgttagg cgagtcgtta
    15661 attcaggatg ccagggcgca ggatctttca ccggaagatt tactcaacaa gaaagcctct
    15721 agtgtgccac ctggctgtca tgggctgatg acggtgctgg actggctgac caatccgtgg
    15781 gaaccgtaca aacgcgggat tatgatcggc tttgattcca gcatggatta cgcatggata
    15841 tatcgttcga tattggaaag tgtggcgcta acgctgaaga acaattacga caatatgtgt
    15901 aatgaaatga atcactttgc gaagcatgtg atcattactg gcggcggttc gaacagcgat
    15961 ctgtttatgc aaatttttgc cgacgtgttc aaccttccgg cacgccgtaa cgccattaac
    16021 ggttgtgcaa gtctgggagc agcgattaat acagcggtag gtctggggct atacccggat
    16081 tacgcaacgg ctgttgataa gatggttcgc gtgaaagata tctttatacc gattgagagc
    16141 aatgccaaac gctacgacgc gatgaataaa ggcattttca aagacctaac caaacatact
    16201 gatgtgatcc tgaaaaaatc gtatgaagtg atgcatgggg aattggggaa tgtggattcg
    16261 atccagagct ggtcgaatgc gtaagtgagg aaggccgggc gggaaactgc ccggcctgaa
    16321 catacctgaa tggttatccc cgctgacgcg gggaacataa gttcgacaac ttaaaaaatg
    16381 ataacaagtc cggtttatcc ccgctgatgc agggaacata ataaaaactt taactagttc
    16441 ggtttatccc tgtttttaca aggaatatta actacactcg atgtctttag aataaaagac
    16501 aatacgaatc gcactttatc a
//
LOCUS       DAPHDW010000101        16333 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.102, whole genome shotgun sequence.
ACCESSION   DAPHDW010000101 DAPHDW010000000
VERSION     DAPHDW010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 16333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16333
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.102"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(375..1571)
                     /gene="hemY"
                     /locus_tag="RH057_003181"
     CDS             complement(375..1571)
                     /gene="hemY"
                     /locus_tag="RH057_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HDV3129512.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(1574..2803)
                     /gene="hemX"
                     /locus_tag="RH057_003182"
     CDS             complement(1574..2803)
                     /gene="hemX"
                     /locus_tag="RH057_003182"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HDV3129513.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKVATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTAAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPA
                     PAPAPQGDTPAAAPQGE"
     gene            complement(2825..3565)
                     /gene="hemD"
                     /locus_tag="RH057_003183"
     CDS             complement(2825..3565)
                     /gene="hemD"
                     /locus_tag="RH057_003183"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HDV3129514.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPRLPDYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTARGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3562..4503)
                     /gene="hemC"
                     /locus_tag="RH057_003184"
     CDS             complement(3562..4503)
                     /gene="hemC"
                     /locus_tag="RH057_003184"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HDV3129515.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLRALVGAPDGSQIIRGER
                     RGAPQNAEQMGISLAEELLNNGAREILAEVYNGDAPA"
     gene            4890..7436
                     /gene="cyaA"
                     /locus_tag="RH057_003185"
     CDS             4890..7436
                     /gene="cyaA"
                     /locus_tag="RH057_003185"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HDV3129516.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPLMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNMPPANQDHDTPLLQQYFS"
     gene            complement(7476..7796)
                     /gene="cyaY"
                     /locus_tag="RH057_003186"
     CDS             complement(7476..7796)
                     /gene="cyaY"
                     /locus_tag="RH057_003186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HDV3129517.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDWDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            8259..8462
                     /locus_tag="RH057_003187"
     CDS             8259..8462
                     /locus_tag="RH057_003187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3129518.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            8499..9323
                     /gene="dapF"
                     /locus_tag="RH057_003188"
     CDS             8499..9323
                     /gene="dapF"
                     /locus_tag="RH057_003188"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HDV3129519.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLAEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            9320..10027
                     /locus_tag="RH057_003189"
     CDS             9320..10027
                     /locus_tag="RH057_003189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HDV3129520.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            10024..10920
                     /gene="xerC"
                     /locus_tag="RH057_003190"
     CDS             10024..10920
                     /gene="xerC"
                     /locus_tag="RH057_003190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HDV3129521.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDVTMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            10920..11636
                     /gene="yigB"
                     /locus_tag="RH057_003191"
     CDS             10920..11636
                     /gene="yigB"
                     /locus_tag="RH057_003191"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HDV3129522.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            11720..13882
                     /gene="uvrD"
                     /locus_tag="RH057_003192"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             11720..13882
                     /gene="uvrD"
                     /locus_tag="RH057_003192"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HDV3129523.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAET
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTLHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARLESV"
     gene            complement(14029..14793)
                     /locus_tag="RH057_003193"
     CDS             complement(14029..14793)
                     /locus_tag="RH057_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HDV3129524.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADMNSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQKVALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKTSKEIQFAVQSGP
                     MLMENSVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKAKLNVE
                     QLLYLDGTISHMYMKGGAIPWQRYPFVTMISVERKG"
     gene            15163..16113
                     /gene="corA"
                     /locus_tag="RH057_003194"
     CDS             15163..16113
                     /gene="corA"
                     /locus_tag="RH057_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HDV3129525.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLTPYLYFKRKNWL"
BASE COUNT         3721 a         4268 c         4407 g         3937 t
ORIGIN      
        1 agctcaggtt aaatattcac tcaggaagtt attactcagg aagcaaagag gattacagaa
       61 ttatctcata acaagtgtta agggatgtta tttcccgatt ctctgtggca taataaacgg
      121 gtagatgctc attccatctc ttatgttcgc cttagtgcct cataaactcc ggaatgacgc
      181 agagccgttt acggtgctta tcgtccactg acagatgtcg cttatgcctc atcagacacc
      241 atggacacaa cgttgagtga agcacccact tgttgtcata cagacctgtt ttaacgcctg
      301 ctccgtaata agagcaggcg tttttttatg catcagaaag gtcccggagg tgcttgcctc
      361 cgggtgagaa ggaactactg tggtgggttg ttctgcaacg ttaacatcaa accgtcgcga
      421 cgcatcgctg cagcttcttc cggcttgtgc agtctgtcca gcgcgtcggc aagccatgcg
      481 taatcgtagg cgtccggacg ttgtttcagc gccgcgcgga aggcgagcga tgcttcctgc
      541 cattctccgt gcttcatcag tgactggccc agtgtgctcc acaacagcgg gcgatcgccg
      601 acgtttttga tctgctggcg cagcactttt tccagctgtt ccggattgtt ggttttcagt
      661 cgaggaatcg gcagcagtag gcgatcgtcg tactggcgtt tcaggccatc gatgataatt
      721 tgctgggcag tatcatgatc gtcacattca ataagatgtt ccgccattgc cacctgcaac
      781 gctacctgat gacgcgtttt ccggctttgg tttttccacc agttacgcaa accttcacta
      841 ccgttatcgg cacgcgcctg atccatcagg ccaatccatg cctgttgttc cagcattgca
      901 cgatgttctt catcaccaac atgggctttc gccattgatg ggatgatatc cagcagagaa
      961 ctccatgcac ctgtgcggat atacgcctgt tccgccagac gtaatacttc cggatggcgt
     1021 ggcgtaactt ccagcagctt atccacgccg tggcgtgcag catggttttc attacgggcc
     1081 agttgcagac gtacacgggt gatttctacc ggaatggtgt cattgccggc cagctctgct
     1141 gcgcgttcca gatgttggtt ggcgcgtgct tcatcaccac gttgttgcgc ggcttcggca
     1201 gccagtagat agttcaccac cggttgttcc gcgtgatcgg catttttcgc catcagcttt
     1261 tcaacttgct gataatcgcc ttccgccagt ttcagcagcg cctgttcggt ctgcttacgg
     1321 gcacggcgac gcttacgtcc gacaaaccac ccacgggtgt gtgcgcctgt gcggaagatc
     1381 cgccgcagta gccactcaat ggcaaacagc actaccatcg ccagaatcaa aataatcgcc
     1441 aggcccgtga cgctggtttc gatattgtag ttgtcagtct ggatcagcac gtaaccctga
     1501 tgaccggcaa tcatcgggcc aaccactatc cccgcaatca gcaaaacaaa gagtaataac
     1561 acttttagca tggttattct ccttgcggcg cggctgccgg agtatcacct tgcggtgcag
     1621 gtgcaggtgc aggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtttag
     1681 cttccgttgt ccccgctgcc ggttgtgcca gcaggttacg cacgcgggtc tgcatcagtt
     1741 tttccagcat cgcctggctt tgcagggttt ccggaagatc catcgagata ttttgctggc
     1801 ttaactggtc cacctcgtcg aggaacgctt tggtggtggc atcatcagta tcgtagtaag
     1861 ctcgtaccca ggtggagacg ttctccagcg cctggcgata agtctcttcc tggtgacgcg
     1921 gtacagcttg tgctgcgacc agcaggcgag agcgaatatt ttcgcgcaga tagatatcct
     1981 gatttggcgc taacagcggt acggcggtgt catcacgacg gcgaatcgta atgaagttgt
     2041 ccataaagtt ctgccagctt ttttgcagat tgatacgcca ttcgctgatg gaactggaaa
     2101 gctcttcacc gtcggaatcc atcggcgaac catcgctgtc attatcggcc agacgcaggt
     2161 tatctacctg atttgaaagc tgattaagct taaggatgat gccgtcataa tccacctgcg
     2221 aaactgcaga aaggctggcg atatcatcgg taattgcccg acgaacggta atcagactcg
     2281 ggtcattcat atccgccagg ctggcatctg cacttttcag caacgctgca gcggtcgtga
     2341 cgtcctgatc gctccacagc ttccgtccgg cgagtttcac cagaaaatcg gcctgagcca
     2401 gcagccaggt tttagcatcg ctgccggaaa tggtggcgac cttttgttgg acttcatcca
     2461 actgttttgc cagcgtttct tgctgacgat tcgcctgctt aagctgtgca gcttgttgct
     2521 taataatgcc ttccagctcg gctttttggc tctcctgggc tttttgcaat gccgtcagtt
     2581 ggttagccag ggcatcgctg gtggcggtct gattgacggc ctgttgttta ccccagccat
     2641 acaaaccgac gcccgccgcc agagcaatag cgatagccac cgcgctgaga atcaatgcgg
     2701 tattgttctt actctttttt tctgttgcga caggttgtga cgtggtgtcc acggcctccc
     2761 tggtctcttc aaccacggcg gaggtttttt cttgttccgt cattatggct tcctgttatg
     2821 agagttattg taatgcccgt aaaagcgcat cgttgtcagc gttatcggcg accttaatgt
     2881 cttgccagcc cagttcccgg gcgagtttcg ccaaacgctc actgacgacc aatagtcgac
     2941 agtgtagtaa ccagtgctca cgataccatt gtgggatcag cgaccagagt tgctgcaaca
     3001 tttcaccgct ggtaacaacg accatcgtca cctcgcggga ttgccagcgc atcgcttctt
     3061 ctgcaccatc gtaatggatt gcgcatcgtt gataacattc acaaaaagtg acctcagcac
     3121 cgcgcgctgt cagggtatcc ccaattagct cacgaccgcc attgccacgt aatatcagcg
     3181 cacgtttgcc cgcaatattt tgtaattcag gtaattgtag caagacttcg ctgatttccc
     3241 gatcctgcgg gtagagaatc ttctgtccgc ttacggtatg tagcgccagt gcggtggtgc
     3301 gtccaatggc gaaataatca ggtagtcggg gccatttacg atcttgctga tgcagctgtg
     3361 attgggcaaa agcaaccgcg tgttgcgaga gggcaaacaa cagatcgctc tcccccagcg
     3421 ccgccagttg atcagcaagt tgcggtaatt gtcgacccgg agaaaactca atcagcggaa
     3481 aatgccaggc cacctgcccc agtgtgcgca gacggctcac taactcttct ccagcgggag
     3541 acgggcgggt gaccaggata ctcatgccgg ggcgtctccg ttatagactt cagcgaggat
     3601 ctcgcgcgcg ccgttattca gtagctcttc tgccagcgaa atccccattt gttcggcatt
     3661 ttgcggcgca ccgcggcgtt caccgcgaat aatctgcgaa ccgtccggcg cgccgaccag
     3721 cgcacgcagc cagatttcac catcaataag ctcggcgtag ctaccaattg gcacctgaca
     3781 tccaccttcg agacgggtat tcatggcgcg ttctgcggta acgcgcagtg cagtttcgtg
     3841 gtgattcagc gcggcaagca gctcgcgagt gcgcgtatca tcaaggcggc attcaatacc
     3901 gactgcacct tgtcctaccg ccggaagaga aatctcgggt ggtaacgcgg cgcgaatgcg
     3961 ggactccaga cctaaacgtt ttagtccggc tacggcaaga atgatggcat cgtattcgcc
     4021 gttatctagt ttgctcaggc gagtgccgac gttgccgcgc agggagcgga taatcagatc
     4081 cgggcggcgt tcagccagtt ggcactggcg acgtaaactg gacgtcccga cgatactgcc
     4141 tgccggtaac gcatccagac tgtcataatt attggacaca aaggcatcgc gaggatcttc
     4201 acgctcacaa atagtgacca accccagacc ttgcgggaat tcaaccggca catctttcat
     4261 tgaatgtacg gcgatatcgg cgcgattctc caggagcgcg acttccagct cttttacaaa
     4321 taagcctttt ccgcctactt tcgccagcgg cgtatcaaga atcacatcgc cgcgcgtcac
     4381 catcggtacc agttcaacca ccaggcccgg atggctcgcc atcaacttgt ctttgacata
     4441 gtgtgcctgc cagagtgcaa gtgggctttg gcgtgtggca attcttaaaa cattgtctaa
     4501 catgcttgtt accgtcatta tcatccgtgg tccatcctaa catccttgcc agagtgatgt
     4561 cagtgttgtg gtgaaacgta gacgcctgcg caaaccgtaa aatgaggtct ggcagtggat
     4621 cctgacaggc gtttcacgcc gtcgtaataa ggaatttaca gagaataaac ggtgctacac
     4681 ttgtatgtag cgcatctttc tttacggtca atcagcaagg tgttaaattg atcacgtttt
     4741 agaccatttt ttcgtcgtga aactaaaaaa accaggcgcg aaaagtggta acggttacct
     4801 ttgacatacg aaatatcccg aatgccgcgt gttaccgttg atgttggcgg aatcacagtc
     4861 atgacgggta gcaaatcagg cgatacgtct tgtacctcta tattgagact ctgaaacaga
     4921 gactggatgc cataaatcaa ttgcgtgtgg atcgcgcgct tgctgctatg gggcctgcat
     4981 tccaacaggt ctacagtcta ctgccgacat tgttgcacta tcaccatccg ctaatgccgg
     5041 gttaccttga tggtaacgtt cccaaaggca tttgccttta cacgcctgat gaaactcaac
     5101 gccactacct gaacgagctt gaactgtatc gtggaatgtc agtacaggac ccaccgaaag
     5161 gtgagcttcc aattactggt gtatacacca tgggcagcac ctcgtccgta gggcaaagtt
     5221 gttcctctga cctggatatc tgggtctgtc atcaatcctg gctcgatagc gaagagcgcc
     5281 agttactaca acgtaaatgt agtctgctgg aaagctgggc cgcctcgctg ggtgtggaag
     5341 tcagcttctt cctgattgat gaaaaccgct tccgtcataa tgaaagcggc agcctggggg
     5401 gcgaagattg tggctccacc cagcatatac tgctgcttga cgaattttat cgtaccgccg
     5461 tgcgtctcgc cggtaagcgt attctgtgga atatggtgcc gtgcgacgaa gaagagcatt
     5521 acgacgacta tgtgatgacg ctttacgcgc agggcgtgct gacgccaaat gaatggctgg
     5581 atctcggtgg cttaagctcg ctttctgctg aagagtactt tggtgccagc ctttggcagc
     5641 tctacaagag tatcgattcc ccatacaaag cggtactgaa aacactgctg ctggaagcct
     5701 attcctggga atacccgaac ccacgtctgc tggcgaaaga tatcaaacag cgtttgcacg
     5761 acggcgagat tgtatcgttt ggtctcgatc catactgcat gatgctggag cgtgttactg
     5821 aatacctgac ggcgattgaa gattttaccc gtctggattt agtacgtcgc tgcttctatt
     5881 taaaagtgtg cgaaaagctc agccgtgaac gcgcctgcgt aggctggcgt cgcgcagtgt
     5941 tgagccagtt agtgagcgag tggggttggg acgaagctcg tctggcaatg ctcgataacc
     6001 gcgctaactg gaagattgat caggtgcgtg aggcgcacaa cgagttgctc gacgcgatga
     6061 tgcagagcta ccgtaatctg atccgctttg cgcgtcgcaa taaccttagc gtctccgcca
     6121 gtccgcagga tatcggcgtg ctgacgcgta agctgtatgc cgcgtttgaa gcattaccag
     6181 gtaaagtgac gctggtaaac ccgcagattt cacccgatct ctcggaaccg aatctgacct
     6241 ttatttatgt gccgccgggc cgtgctaacc gttcaggttg gtatctgtat aaccgcgcgc
     6301 caaatattga gtcgatcatc agccatcagc cgctggaata taaccgttac ctgaataaac
     6361 tggtggcgtg ggcatggttt aacggcctgc tgacctcgcg cacccgtttg tatattaaag
     6421 gtaacggcat tgtcgatttg cctaagttgc aggagatggt cgccgacgtg tcgcaccatt
     6481 tcccgctgcg cttacctgca ccgacaccga aggcgctcta cagcccgtgt gagatccgcc
     6541 atctggcgat tatcgttaac ctggaatatg acccgacagc ggcgttccgc aatcaggtgg
     6601 tgcattttga tttccgtaag ctggatgtct tcagctttgg cgagaatcaa aattgcctgg
     6661 taggtagcgt tgacctgctg taccgcaact cgtggaacga agtgcgtacg ctgcacttca
     6721 acggcgagca atcgatgatc gaagccctga aaactattct cggcaaaatg catcaggacg
     6781 ccgcaccgcc agatagcgtg gaagtcttct gttatagcca gcatctgcgc gggttaatcc
     6841 gtactcgcgt gcagcaactg gtttctgagt gtattgaact gcgcctttcc agcacccgcc
     6901 aggaaaccgg gcgtttcaag gcgctgcgcg tttctggtca aacctggggg ttgttcttcg
     6961 aacgcctgaa tgtatcggta caaaaactgg aaaacgccat cgagttttat ggcgctattt
     7021 cgcataacaa actgcacggc ttgtcagtgc aggttgaaac caatcacgtc aaattaccgg
     7081 cggtggtgga cggctttgct agtgaaggga tcatccagtt cttcttcgaa gaaacgcaag
     7141 acgagaatgg ctttaatatc tacattctcg acgaaagcaa ccgggttgag gtgtatcacc
     7201 actgcgaagg cagcaaagag gaactggtgc gtgacgtcag tcgcttctac tcgtcatcgc
     7261 acgaccgctt cacctacggc tcaagcttca tcaacttcaa cctgccgcag ttctatcaga
     7321 ttgtgaaggt tgatggtcgt gaacaggtga ttccgttccg cacaaaatct atcggtaaca
     7381 tgccgcctgc caatcaggat cacgatacgc cgctattaca gcaatatttt tcgtgatgaa
     7441 cgtgccggaa agcgaggctt atccggcatg caatcttagc ggaaactgac tgtttcaccc
     7501 gcttgctgtg tcgccgcctg ttccagcaaa tcccagaagg tttcgccgct gcgatcgcaa
     7561 atccattcat cgccttttag gtcaaaatgg tagccgccct gtttggttgc cagccacacc
     7621 tggtgcagcg gctcctggcg gttaataatg attttgctgc cgttttcaaa ggtaatggtc
     7681 agtacgccgc cgttgatttc gcagtcgata tcgctgtcgc catcccagtc gtccaggcgt
     7741 tcttcaatgg tcagccagag ttgatcagcc aggcgatgaa attcactgtc gttcattgtt
     7801 gtatcctgtt tttaagtgat ggcggcagta tagcggcatg gggtcagggc ttcaaagttt
     7861 gcacctctgc ggctgcgttc cggcacgatt catccgtcac cggaataatg atgtctcagt
     7921 gtagcgaaag atttgtctct tcattagggc gcagttacac cacgcctttc cctgtttctg
     7981 gtaaacatta tgatcaggtt taccgagcga gcatcctcac gctgacggaa ctaaaaaagc
     8041 caacaaacaa actacattgc gatagtgcat aaagccatcc tggcgcgagg tgccgatcac
     8101 gaaactacca gcaaaacata aatccccacg agtaagcgtt atactcgcag catttcctca
     8161 cttttcagac ttcataaaga gtcgctaaac gcttgctttt acgtcttctc ctgcgatgat
     8221 agaaagcaga aagcgatgaa ctttacaggc aatccataat gaaaaacgtg tttaaggcac
     8281 tcactgtatt acttactctc ttcagcctga cgggctgcgg tctgaaaggt ccgctctatt
     8341 tcccgcctgc agataaaaac gcaccgccgc cgaccaaacc ggtagagacg caaacgcaat
     8401 ccacggtgcc ggataaaaac gatcgcgcca ctggcgatgg tccatcccag gtgaattact
     8461 aaaagtcagt ttctgtaccc gcgtgattgg agtaaatgat gcagttctcg aaaatgcatg
     8521 gccttggcaa cgattttatg gtcgtcgacg cggtaacgca gaatgtcttt ttttcaccgg
     8581 agctgattcg tcgcctggct gatcggcacc tgggagtagg gtttgaccaa ctgctggtgg
     8641 ttgagccgcc gtatgatcct gaactggatt ttcactatcg cattttcaat gctgatggca
     8701 gtgaagtggc gcagtgcggc aatggtgcgc gctgctttgc ccgttttgtg cgtctgaaag
     8761 ggctgaccaa taagcgcgat atccgcgtca gcaccgccaa cgggcggatg gttctgaccg
     8821 tcaccgatga tgatctggtc cgcgtaaata tgggcgaacc caacttcgaa ccttccgccg
     8881 tgccgtttcg cgctaacaaa gcggaaaaga cctatattat gcgcgccgcc gagcagacaa
     8941 tcttatgcgg cgtggtgtcg atgggaaatc cgcattgcgt gattcaggtc gatgatgtcg
     9001 ataccgcggc ggtagaaacg cttggtcctg ttctggaaag ccacgagcgt tttccggagc
     9061 gagccaatat cggttttatg caagtggtta agcgcgagca tattcgttta cgcgtttatg
     9121 agcgtggggc aggagaaacc caggcctgcg gcagcggcgc gtgtgcggcg gtggcggtag
     9181 ggatccagca aggtttgctg gccgaagaag tacgcgtgga actccccggc ggtcgtcttg
     9241 atatcgcctg gaaaggtccg ggtcacccgt tatatatgac tggcccggcg gtacatgtct
     9301 acgacggatt tattcatcta tgaagcaacc aggggaagaa ctgcaggaaa cactcacgga
     9361 gcttgatgac cgggcggttg tcgattatct gattaaaaat cctgagtttt ttatccgtaa
     9421 tgcgcgcgca gtagaagcga tacgtgtgcc gcatccggta cgcggcaccg tttcgttggt
     9481 cgaatggcac atggcccgcg cacgtaatca tattcatgtt ctggaagaga acatggcgtt
     9541 gttgatggaa caggctatcg ccaacgaagg cctgttttat cgcttactct acctgcagcg
     9601 cagtctcacc gccgccagca gtctcgacga tatgctgatg cgctttcacc gctgggcgcg
     9661 cgatctcggt ctggcaggtg cgagtctgcg cctgtttccg gatcgctggc gcttaggtgc
     9721 gccgtcgaac cacactcatc tggcattaag ccgtcagtct ttcgaaccgc tgcgtattca
     9781 gcgtttgggg caggaacagc actatcttgg gccgcttaac ggaccagagc tgctggtggt
     9841 gctaccggaa gcgaaagcgg tgggatcggt ggcgatgtcg atgctgggaa gcgatgctga
     9901 tttgggtgtc gtgctgttta ccagtcgcga tgccagtcac tatcaacaag ggcaaggaac
     9961 gcagttactt catgaaattg cgctgatgtt gccggagctt ctggagcgtt ggattgaacg
    10021 cgtatgaccg atttacacac cgatgtagaa cgctacctac gttatctgag cgtggagcgc
    10081 cagcttagcc cgataaccct gcttaactac cagcgtcagc ttgaggcgat catcaatttt
    10141 gccagcgaaa acggcctgca aagctggcag caatgtgatg tgacgatggt gcgcaatttt
    10201 gctgtacgca gtcgccgtaa agggctggga gcagcaagcc tcgcgttacg gctttctgcg
    10261 ctacgtagct tttttgactg gctggtcagc cagaacgaac tcaaagctaa cccggcgaaa
    10321 ggtgtttcgg caccgaaagc gccgcgtcat ctgccgaaaa atatcgacgt cgacgatatg
    10381 aatcggctgc tggacattga tatcaatgat cccctcgctg tacgcgaccg tgcaatgctg
    10441 gaagtgatgt acggcgcggg tctgcgtctt tctgagctgg tggggctgga tattaaacac
    10501 ctcgacctgg agtctggtga agtgtgggtt atggggaaag gcagcaaaga gcgccgcctg
    10561 ccgattggtc gcaacgctgt ggcgtggatt gagcactggc ttgatttgcg cgacctgttt
    10621 ggtagcgaag acgacgcgct ttttctgtcg aaactgggca agcgtatctc cgcgcgtaat
    10681 gtgcagaaac gctttgccga atggggcata aaacaagggc tgaataatca cgttcacccg
    10741 cataaattac gtcactcgtt cgctacgcat atgctggagt cgagcggcga tcttcgtggt
    10801 gtgcaggagc tgctgggtca tgccaacctc tccaccacgc aaatctatac tcatcttgat
    10861 tttcaacacc ttgcctcggt gtacgatgcg gcgcatccac gcgccaaacg ggggaaataa
    10921 tgcgttttta ccggcctttg gggcgcatct cggcgctcac ctttgacctg gatgataccc
    10981 tttacgataa ccgtccggtg attttgcgca ccgagcgaga ggcgcttacc tttgtgcaaa
    11041 attatcatcc ggcgctgcgc agcttccaga atgaagatct gcaacgcctg cgccaggcgg
    11101 tacgggaagc ggaacccgag atttatcacg acgtgacgcg ctggcgtttt cgttcgattg
    11161 aacaagcgat gctcgacgcc gggctgagtg ccgaagaagc cagtgcaggc gcacacgcag
    11221 caatgattaa ctttgccaaa tggcgcagcc gaatcgacgt cccgcagcaa actcacgaca
    11281 ccttaaaaca gctggcgaag aaatggccgc tggtggcgat caccaacggt aacgcccagc
    11341 cggaactgtt tggtttgggg gattattttg agtttgtgct gcgcgctggc ccgcacgggc
    11401 gctcaaaacc gttcagcgat atgtactttt tggctgcgga aaaactcaac gtgccgatcg
    11461 gcgagatctt acatgttggg gacgatctca ccactgacgt gggtggggca attcgcagcg
    11521 gaatgcaggc ttgttggatc agaccggaaa atggcgatct gatgcaaacc tgggacagcc
    11581 gtttactgcc gcatctggaa atttcccggt tggcatctct gacctcgctg atataatcag
    11641 caaatctgta tatataccca gctttttggc ggagggcgtt gcgcttctcc gcccaaccta
    11701 tttttacgcg gcggtgccaa tggacgtttc ttacctgctc gacagcctta atgacaaaca
    11761 gcgcgaagcg gtggccgcgc cacgcagcaa ccttctggtg ctggcgggcg cgggcagtgg
    11821 taagacgcgc gtactggtgc atcgtatcgc ctggctgatg agcgtggaaa actgctcgcc
    11881 atactcgatt atggcggtga cgtttaccaa caaagcggcg gcggagatgc gtcatcgtat
    11941 cgggcaactg atgggcacca gccagggcgg catgtgggtc ggcaccttcc acgggctggc
    12001 gcaccgcctg ctgcgtgcgc accatatgga cgccaatctg ccgcaggatt tccagatcct
    12061 cgatagcgag gaccagctgc gcctgcttaa gcgtctgatc aaagccatga acctcgacga
    12121 gaagcagtgg ccgccgcgcc aggcaatgtg gtacatcaac agccagaaag atgaaggcct
    12181 gcgtccgcat catattcaaa gctacggtaa tccggtggag cagacctggc agaaggtgta
    12241 tcaggcgtat caggaagcgt gtgaccgcgc gggcctggtg gacttcgccg agctgctgct
    12301 gcgcgctcac gaactgtggc ttaacaagcc gcatatccta caacactacc gcgaacgttt
    12361 taccaatatc ctggtggacg aattccagga taccaacaac attcagtacg cgtggatccg
    12421 cctgctggcg ggcgacaccg gcaaagtgat gatcgtcggt gatgacgacc agtcaatcta
    12481 cggctggcgc ggggcgcagg tggagaatat tcagcgtttc cttaatgatt tccccggtgc
    12541 cgaaactatt cgtctggagc aaaactaccg ctctaccagc aatattctga gcgccgctaa
    12601 cgccctgatt gaaaacaata acgggcgtct gggtaaaaaa ctgtggaccg atggcgcgga
    12661 cggtgagcct atttccctct attgcgcttt taacgaactc gatgaagcgc gttttgtggt
    12721 taaccgcatc aaaacctggc aggacaacgg cggggcgctt gccgagtgcg ccattctcta
    12781 ccgcagtaac gcccagtcgc gtgtactgga agaagcactg ttgcaggcca gtatgccgta
    12841 ccgtatttac ggcgggatgc gcttcttcga acgccaggaa atcaaagacg cgctctcgta
    12901 tctgcgtctg attgccaacc gtaacgacga cgcggcgttt gagcgcgtgg tgaatacgcc
    12961 aacgcggggt attggtgacc ggacgctgga cgtggtacgt cagacatcgc gcgatcgcca
    13021 gttaacactc tggcaggcat gtcgtgaact gttgcaggaa aaagccctcg ccgggcgagc
    13081 tgccagcgcc ttgcagcgat ttatggaatt aatcgatgcc ttagcgcaag aaactgccga
    13141 tatgccgctg catgtacaga ctgaccgggt aattaaagac tccggcctgc gcaccatgta
    13201 cgagcaggag aagggcgaaa aaggccagac gcgtatcgaa aacttagagg aactggtgac
    13261 ggcaacgcgc cagttcagct acaacgaaga agatgaagat ttaatgccgc tgcaggcatt
    13321 cctctcccat gcggcgctgg aagcgggcga ggggcaggcg gatacctggc aggatgcagt
    13381 gcagttgatg acgctgcact cggcgaaagg cctggagttc ccgcaggtgt ttattgtcgg
    13441 tatggaagag ggtatgttcc caagccagat gtcgctggat gaaggcgggc gtctagaaga
    13501 agaacgccgt ctggcctacg ttggtgtaac ccgcgcgatg cagaaactga cgctgaccta
    13561 cgcggaaacc cgccgtctgt atggtaaaga ggtttaccat cgcccgtcgc gctttatcgg
    13621 cgagctgccg gaagagtgtg tggaagaggt gcgcctgcgc gctacggtaa gtcgtccggt
    13681 cagccatcaa cggatgggga cgccgatggt cgagaacgac agcggctaca agctcggtca
    13741 gcgtgtacgc cacgctaagt ttggtgaagg caccatcgtc aatatggaag gcagcggcga
    13801 gcatagccgt ttgcaggtgg cattccaggg ccagggtatt aaatggctgg tggcggcata
    13861 cgcccggctg gagtcggtgt aacgttgccg gatgcggtgc tgcgcacctt atttggccta
    13921 aaaaatcatt catattcaat aaattgcaac gtcatgtagg ccggataggg cgtttacgcc
    13981 gcatccggca tctgcgccat cttcagtatc tgacacaaaa ctatcgtttt aacctttccg
    14041 ctctacggaa atcatggtga caaaaggata gcgttgccac gggattgccc cgcctttcat
    14101 atacatatgt gaaatcgtgc catcaagata aagcagctgc tcaacgttca gtttcgcttt
    14161 ggcataacag gcaaaatcat aaaaatttgt cgcctgctgg ctcaacaaaa acacggcatt
    14221 cccatgttta ttaatcccaa caccgttacg aattttacgt gaggcgacgt tgggatgaat
    14281 acgcggatta attacactgt tttccatcaa cattggccct gactgcaccg caaactgaat
    14341 ctctttactg gttttgaagg catccagacg aacgatgccg actttatctc ccgcgacata
    14401 aaacacgccg ccaggacgga taaagaaatt cccttcacct gaagcgagat ttaacgccac
    14461 cttctgctga ccgttttcga tgtacaaacc gagcggcgca tagctttcat catagatgcc
    14521 gccgttcatc gccatctgca cctgaccctg actattcata tccgccagca gagcatgtaa
    14581 agttccccac gcttcgccat tggctttttg ccagtacatt ttcacccgct ctgtttgagg
    14641 attaacggta tacgcctgta cggtcagcgt cggatctgag agtgcgcaat catcagcggc
    14701 aacagcaaac aagggaagta aggtgagggt gagaaaaatc cgtttgagat tcaaggtgat
    14761 cattccttta ccaatgagta gccgatgcgc cattataggt cctggatgtt ggattttttt
    14821 atcctgttag cgaccttgac gagtaccaaa aagcgcgaag ttcaactatt gttctgtggt
    14881 gttctgttgc gtgttgacgg caaaattttg ctggcgtaac atgcgcgcac gatcactcta
    14941 agaggacatt cgccttggac acacccagta gatactggct cactatcctg tcatccagga
    15001 tcaactccta aggctatccc tttttgctga tagccttagc ggttgtcagc gacctcaatt
    15061 tttcccgtcg cgctgagtca ggctgtttaa tggtctgaaa cccaatttgt ttctgtgtgc
    15121 ccaccgaact gtccgatatt ttaagcattg ggagtcccgg tcatgctgag cgcatttcaa
    15181 ctggaaaata accgactgac ccggctggaa gtcgaagagt cacaacccct tgtaaatgca
    15241 gtatggattg atcttgtcga accggacgac gacgagcgac tgcgcgtaca atctgaactt
    15301 ggccagagcc tggcaacccg cccggaactg gaagacatcg aagcatcggc acgtttcttt
    15361 gaagacgacg acggcctgca tattcactcc ttcttcttct ttgaagatgc ggaagatcac
    15421 gccggtaact ccactgtggc atttaccatc cgtgatggtc gtctgtttac tctgcgtgag
    15481 cgtgaactgc ctgcattccg tctgtatcgt atgcgcgccc gtagccagtc aatggtggac
    15541 ggtaacgcct acgagctgct gttggatctg ttcgaaacca aaatcgaaca gctggcagat
    15601 gaaattgaaa atatctacag cgatctggag cagttgagcc gggtaattat ggaagggcat
    15661 cagggcgatg agtacgatga ggcgctctcc acgctggcgg aactggaaga tatcggctgg
    15721 aaagttcgcc tgtgtctgat ggatacccag cgtgcgctca acttcctggt acgcaaggcg
    15781 cgtttaccgg gtgggcaact ggagcaggcg cgtgaaatcc tgcgagatat cgaatccctg
    15841 ctgccgcata acgaatccct gttccagaag gtgaacttcc tgatgcaggc ggcgatgggc
    15901 tttatcaaca tcgagcagaa ccgcatcatc aaaatcttct cggtggtatc cgtggtgttc
    15961 ctgccaccga cgctggtggc ttccagctac gggatgaact ttgaatttat gccagagttg
    16021 aagtggagtt tcggctaccc aggcgcgatt atctttatga tcctcgcggg cctgacaccg
    16081 tatctgtact ttaagcgtaa gaactggctg taactatacg atgtcgctgt gagtaagtta
    16141 gtgtgctgac agcgacattt tagccgaggg aatcgcagat aacataattg ttgtgaataa
    16201 tatccatgcc acgttgagtt ggacgcttaa tgactcacat ctcacccctt caattgctgc
    16261 agagataata gaaaaaagtg tcaagcccaa tgaaatcaat acaaagtaga ttgccttaca
    16321 tccagtatct ctt
//
LOCUS       DAPHDW010000102        15464 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.103, whole genome shotgun sequence.
ACCESSION   DAPHDW010000102 DAPHDW010000000
VERSION     DAPHDW010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15464
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.103"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(298..1209)
                     /gene="gsiD"
                     /locus_tag="RH057_003195"
     CDS             complement(298..1209)
                     /gene="gsiD"
                     /locus_tag="RH057_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HDV3129526.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGAAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(1212..2132)
                     /gene="gsiC"
                     /locus_tag="RH057_003196"
     CDS             complement(1212..2132)
                     /gene="gsiC"
                     /locus_tag="RH057_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HDV3129527.1"
                     /translation="MLNYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMLLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(2150..3688)
                     /gene="gsiB"
                     /locus_tag="RH057_003197"
     CDS             complement(2150..3688)
                     /gene="gsiB"
                     /locus_tag="RH057_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HDV3129528.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAAAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPAALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPTTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(3708..5579)
                     /gene="gsiA"
                     /locus_tag="RH057_003198"
     CDS             complement(3708..5579)
                     /gene="gsiA"
                     /locus_tag="RH057_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HDV3129529.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLQRRSREVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYPHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQAPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGPRRAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(5566..6531)
                     /gene="iaaA"
                     /locus_tag="RH057_003199"
     CDS             complement(5566..6531)
                     /gene="iaaA"
                     /locus_tag="RH057_003199"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HDV3129530.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFARGMERVSPEIFSTPLRYEQLLAARKEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            6735..7970
                     /gene="moeA"
                     /locus_tag="RH057_003200"
     CDS             6735..7970
                     /gene="moeA"
                     /locus_tag="RH057_003200"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HDV3129531.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTAQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASRQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVISKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKTILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVGEWVEVEPFNALFGGL"
     gene            7970..8719
                     /gene="moeB"
                     /locus_tag="RH057_003201"
     CDS             7970..8719
                     /gene="moeB"
                     /locus_tag="RH057_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HDV3129532.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALARINPHIA
                     ITPVNALLDDAELAAMIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(8807..9469)
                     /gene="fsa"
                     /locus_tag="RH057_003202"
     CDS             complement(8807..9469)
                     /gene="fsa"
                     /locus_tag="RH057_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308930.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HDV3129533.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLEVVLP
                     ELHEAMGGQGRLFAQVMATTAEGMVNDARKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVAKFEQDWLGAFGRT
                     SI"
     gene            9600..10499
                     /locus_tag="RH057_003203"
     CDS             9600..10499
                     /locus_tag="RH057_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HDV3129534.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHFTALTDCCPTQALTVCGE
                     VKSVEEIMTTVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQASHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            10505..12937
                     /locus_tag="RH057_003204"
     CDS             10505..12937
                     /locus_tag="RH057_003204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HDV3129535.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHELCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIVLVAVSE
                     HIERFAALAREMAATETRESRRDELLTIAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVQVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            13083..13898
                     /gene="ybiV"
                     /locus_tag="RH057_003205"
     CDS             13083..13898
                     /gene="ybiV"
                     /locus_tag="RH057_003205"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HDV3129536.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGNQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHVALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            14050..15315
                     /locus_tag="RH057_003206"
     CDS             14050..15315
                     /locus_tag="RH057_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HDV3129537.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLDRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRRPPGTTSKGLGAHTDSGALERWLLPAYQ
                     RVFANVFNGNLAQYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
BASE COUNT         3735 a         4274 c         3941 g         3514 t
ORIGIN      
        1 taccgcgcgt tccgatacca gttgatagtt aatgatgaaa aaaaggccaa taaatgacag
       61 tgctgccaat aaaaataaaa ttatcagcct tatctttatc ttttcgtata aactcagcat
      121 gttaatacat ttaacaaaat aactgtctga acgctgtgcc gctgcaaaaa tagtcccttg
      181 ctgcggcttt ttaaccaaat tacccgattc tacaaaatca gcagaaagtt acaacacttg
      241 cttagggcaa taatttcctg tgaggtgatt accctttcaa gcaatattca aacgtaacta
      301 tcctttaatt ttcggatcca gcgcatcgcg taaaccatcg cccaacaaat tgaacgccag
      361 tacggtcaga aaaatagcca gggccggaaa aacagcgaca tgcggcgcga taaccatatc
      421 cgctcgtgcc tcattgagca ttgctcccca ctctggtgtc ggcggctgcg caccgaggcc
      481 gagaaatgac aggctggcgg cagagataat cgaggtgcca atgcgcatgg tgaaaaacac
      541 cacgatagac gagacggtcc caggcaggat atgacgcaat aaaatggtca tatcgctggc
      601 accaatactg cgtgctgact caataaaggt ttgctgtttc aacaccagcg tgttgccgcg
      661 caccaggcgg gcaaacgcgg ggatggaaaa aatggcgact gcgataatca cgttagcaat
      721 gccgcttccc aacaccgcaa caacagcgat cgccagtaaa atacccggga aggcaaacag
      781 cacatcgcaa atgcgcatga tcagccgatc ccaccagcct tcataatatc cagccagcaa
      841 gcccagcaac gtccctatcg ccgcaccgat aaacacggca aacacgcccg ccgccagcga
      901 gatttgcgca ccaaccagga cacggctgaa aatgtcgcgc cccagtgaat cgacgccaaa
      961 ccagtgctgc aaagaaggtc cgttattcag attgtcataa tcaaaataat tttcggcgtc
     1021 atagggagcg atccagcgtg caaaaatggc caccacaatc aataaaataa cgaataatgc
     1081 ggcggtcatc gccatatgct gacggcgaaa tcgtcgccag aattcatgcc acggtgtacg
     1141 tacctggtca ggtttgacca gtggcatggc gtttaacacc gcctgacgtc gccagttaaa
     1201 tagtcgcatc cttacttgta cctgatagcc gggttaatgg cggcgtaaag cacatccacc
     1261 actaagttga taagaataaa ttccagtgag aaaagcagaa tttccgcctg aatcaccggg
     1321 taatcgcgca tttctacgga gtcaacgagt aagcgtccaa gtccgggcca gttgaaaact
     1381 ttctccacca cgatagaacc accgagcaaa aagccaaact gtaagcccat catggtcact
     1441 accgggatca tcgcgttgcg tagcccgtgt ttgaggacaa cccaggtttc gctcacccct
     1501 ttcgccctcg cggtacgcat ataatcttcg cttaaaacat cgacaaacga cgcgcgggta
     1561 aagcgcgcca tcacggctgc cactgccgcg ccgagcgtca gggagggtaa aatgtagtgc
     1621 tgccaactgt ctgctcccac ggtaggcagc cagcccagtt caacggagaa tacctgaatt
     1681 aaaagcatcc ccagcgcaaa tgccggaaac gagatccccg acaccgcaat ggtcatactc
     1741 aatcgatccg gccaacgatt acgccagacg gcggcgataa ttcccgccgc cataccaaat
     1801 atcaccgccc agaccatact ggttatggtc agccacagcg ttggcataaa gcggctggca
     1861 atctcatcgg caaccggacg acgcgacacc atcgacaggc caaaatcccc ctgcacagca
     1921 ttgctgatat agtgccagaa ctggtgatac agcggctgat ccaaccccag ctgctgacgc
     1981 accagttcta taacctgcgc atcagcttcg ggcccggcaa tcaatcgcgc cggatcgccg
     2041 ggcagcatgt ggacaaataa aaacaccagc accgagacga taaacagcgt cggaatcaac
     2101 cccagtaatc gtttgataac gtaattaagc attccactcc ctgcgttgct tattgcaaat
     2161 ccgcgtcttc aaagctgaag ccggtgtctg gcatgatcca aaaaccggtc aggtttttac
     2221 tgtgtgccga caccagtttt tctaccacca gcgggatcca cggcgactct tgccagatga
     2281 tatcctgcgc cgccttatac aagcgggtct tttccgccgg atcattcgtt ttcagtgcct
     2341 gagccaggaa gtcgtccacc tgtttattgc tgtaaaatgc ggtattaaac agcgtcgttg
     2401 gccagttttg cgaggcgaac agtggcgata gcgcccagtc cgcttcgccg gttgaagccg
     2461 accagccagt gtagaacatc cgcacgccgc tctctttttg ccctttacct tcaacttctg
     2521 ccgcccgctg tccggcatcc atcgcggtca cctgggcttt aatcccgacc tgcgctaact
     2581 gctgctgggt aaattgcagc actttctgcg cggtgctgtg gttatgtgac gaccacagcg
     2641 tggtactgaa accgttggga tatcccgcct ctttcagtaa ttcgcgcgct ttcactggat
     2701 cgtaaggcca cggtttataa ctttgcgcgt aggcgatact tggcggtacc acaccagtag
     2761 ctggcgttgc atagcccgca aaggcaactt tcaccagcgc cggacggtta atggcgtaat
     2821 tcagcgcctc acggaccttc gggttatcga acggcttttg cgtcacgttc atactgatat
     2881 aacgctgcat aattgacgga ctggccatca actcgatatt tttgtttttc tccagcagtg
     2941 tggcctgctc gtagggaatg gggaaagcaa actgcgcttc accggtttgc agcattgccg
     3001 cgcgggtgtt gttatccgcc accggacgcc aggttatgct gtccagtttg ggcaatcctg
     3061 gctgccagta acccgcgaat tttttcacct tcacaaaatc ggtctgattc caggtatcca
     3121 gttcatacgg tccggttccc accggatgaa aaccaatctc cttgccatat ttttccagcg
     3181 ctgccggtga aatcatcgcg gtcgccggat gggcaagaat attaataaac gctgagaacg
     3241 gctgtttgag ggtaatcttt actgttgttg gatcgatcgc ctccgttttg gcaatattct
     3301 tatacaggtt atagcgttta agatgattcg ccggatcgct ggcccggtcc agattcgctt
     3361 tcaccgccgc ggcgttgaaa tcggtgccat cctggaattt aatgccttcc cgcaatttca
     3421 cggtgtaagt aatgccgtca tcggaaacgg tataactctc cgccagcacg tttttcagtt
     3481 tcatctcttt atccagaccg aacagcccct ggtaaaacga tttcgctacg gcctgagata
     3541 acgtgtcatt cgcgtcatac ggatcgagcg tggtgaaatt cgatcctacc gccaccacca
     3601 catctttggc agcgaatgca caagatgcca tcaacgctgt cgcaatgccc agcgccacta
     3661 acccactacg gtgtacagct cttgccattt tattctccgc ctgaatgtta tctacgcatg
     3721 aatgcgtatt ctgattgtgg ttgtgcgacg taatgccccg gcccgacgca ttgcaacgag
     3781 acggctgcca cctcttcgcc acgcagatga atattgctgg gaagatcgtc cgacagcagt
     3841 acacgctgcg gtcgttgtcg ggacggttca gcgaccggaa ctgccgccag taatttacgc
     3901 gtataaggat gctgcgggtt ttcgaagacc gcgcgccgtg gaccaatttc aacaatttgc
     3961 ccgagataca tcaccgccac acgatgacta atccgctcta ccacggccat atcgtgggag
     4021 ataaacagat acgcaatgcc gaaatcacgc tggagatcga gcaacaagtt gataatctgc
     4081 ccgcgaatag aaacatccag cgccgaaacg gcttcgtcgg caatgatcac ttttggattc
     4141 aatgccaacg cgcgagcaat gcagatgcgc tggcgctgac cgccggaaaa ctcatgcggg
     4201 taacgccagg catgttcagg taacaggccc acgcgctcca gcaaccacgc aacgcgtgca
     4261 gccgcgtctt tacctggcaa taaaccgtgt acacgcagcg gttcgataat cgaatcaccg
     4321 atggtctgac gtgggtccag cgaagcgtaa gggtcctgaa aaataaactg aatatcccgg
     4381 cgcaatgcct gaagtttgcc gggtgacaag gtatcgattc tctgaccgtt aaagataatt
     4441 tcgccgccct gcgattcgac caggcgcagc aacgcccgcc cggtagtgga tttaccgctg
     4501 ccagactcgc ccaccagcga tagcgtttcg ccaggccaga gatcaaaact gactttctca
     4561 acggcatgca cttcccgcgt tacgcgattc aacaaaccgc tgcgcaaagg gaaacgggtg
     4621 acaagattac gcactcgtaa aacaggttcg ccatccacca ccgttttctg ctcgatgggg
     4681 ggggcctgtt tcgctggatg ttcaagcgat atcaacggga aacgtcgggg ataatctaac
     4741 cctttcatcg caccaagttg cggaacagca gctaacagcg cacgggtgta aggatgttgc
     4801 ggtgcatgaa aaatctgttc gacggtaccc gtttccaccg cctcgccctg atacatcacc
     4861 agtacccgat cggcaatctc tgccaccacg cccatatcgt gagtgataaa gataacgccc
     4921 atcgacatct ctttttgcaa tactttgatt aattgcagga tctgcgcctg aatagtgaca
     4981 tccagcgcgg tggttggctc atcggcaatc agcaccgccg ggcggcatga cagcgccatc
     5041 gcaatcatca ctcgctggcg catcccgcca gagagttgat gcggataacg tgaaagaatg
     5101 gtttgtgcct caggaatgcg tacctgatcc agcatccgct tcgcctcgac catcgcttct
     5161 tcacgactgg cgttctgatg cagacgaatt gattcggcaa tctgttcacc cacagtaaat
     5221 accgggttca gcgatgtcat cggctcctga aatatcatcg ccatatccgc accgcgaaca
     5281 tggcgcattt gtgcagcgct ctgctcgcta agttcaatca cttcgcgact gcgccgctgc
     5341 aacagcattt tatcgcactg tactaaaccg cccgcctgtt ccaacaggcg catcaatgcc
     5401 aacgcagtca ctgacttacc ggagccggat tcgccaacaa ttgccagcgt ctcaccgcgt
     5461 tgcagactaa aagagagatt gcggaccgca gctattttct gctggtcctg cataaaggca
     5521 atattcagat tttcaaccgc cagcacatta ccggcatcaa gttcatcact gtgtggcaac
     5581 ggtgtcccct ttttcacggt agataccggt ggttggcgta tcgcctgcgt agccccaggc
     5641 gcgatacatt ccttcggtgt taaacggtag cgcgacattc ccttcatggt cgatagcgat
     5701 taagccaccg ctaccgccaa gcgcagggag tttttccatt actacccgct cgcaggcttc
     5761 cgcgagactt aatccgccgt aatccattaa cgcggcgatg tcatatgccg ccagcgcgcg
     5821 gatgaagact tcgcccgtgc cggtacaaga aaccgccaca ctggcgttat tggcgtagca
     5881 tccggcaccc actaagggac tatcgccaac tcgtccgggt aatttattgg tcattccgcc
     5941 tgtggacgtg gctgccgcca aattgccgtc taaatccaac gccacggccc ccacggtgcc
     6001 cattttctgt ttttcatcca gtggcgcacc gctatggtcg aggacggttg ccccttcctt
     6061 gcgcgctgcc agtagttgtt cataacgcaa aggcgtggag aaaatctccg gcgagacgcg
     6121 ctccatgcca cgagcaaacg caaaattttc tgccccttcg ccaatcatca tcacatgcgg
     6181 gctttgctcc atcaccagcc gggcggcaag aaccggatta cgcagatgac taacgcccgc
     6241 caccgcaccg gctttcaggg tgttaccatc catcacacag gcgtccagtt catgggtttc
     6301 atcacgcgta aagacagcgc caattccggc gttaaacagt ggacactctt ccagcagacg
     6361 caccgcttcc gtcaccacat ccagcgcact ttcgcccgct tccagcattt tctgcccggt
     6421 ttcaacaatg gcagacaacg cctcaatgta gcgtaattcc tgttgcagac tcatctgcgc
     6481 gcggctaatt gcacctgcgc caccatgaat tgcaatgact gctttgccca tagtattcgt
     6541 ccattaaggg tggctcgcca cttttgctat aaatatcaga atttttatga attactcatt
     6601 caatttttga atatagaaag atggatatgg catgtaaagg caggagccaa cgctacgccc
     6661 gaggtggcat acagtctgtg gcgtttttct gacataatag gcaaattcga ttttgcctcc
     6721 gaaggagtgt tttcatggaa tttaccaccg gattgatgtc gctcgacacc gcgcttaatg
     6781 agatgctttc tcgcgtcacc ccactgaccg cccaggaaac gctgccactg gtacagtgtt
     6841 ttggtcgtat tctggcgagc gatgtcgttt cgccgcttga tgttccgggg tttgataact
     6901 ccgcaatgga cggctacgcg gtgcgtttag ccgatattgc ctccaggcaa ccgctgcccg
     6961 ttgccggtaa atcctttgcc ggtcagccgt accatggtga atggcctgcg ggtacctgca
     7021 ttcgtattat gaccggtgcg ccggtgccgg aaggctgcga agcggtggtg atgcaggagc
     7081 agactgaaca aacggacaat ggcgtgcgtt ttactgctga agtgcgtagc gggcaaaata
     7141 ttcgccgtcg cggtgaagat atctctgcag gtgcggttgt tttcccggcg ggaactcgcc
     7201 tgactaccgc tgaactgcca gtgattgctt cactggggat tgccgaagtt ccggtgatta
     7261 gtaaagtgcg tgtagcgctt ttttctaccg gtgatgaact ccagttgccc ggtcagccac
     7321 tgggcgatgg ccaaatctac gataccaacc gtctcgccgt acacctgatg ttagaacagt
     7381 tgggatgcga ggtaatcaac ttagggatta tccgcgacga tccacatgcc ctgcgcgccg
     7441 catttattga agccgacagc caggcggatg tggtgatcag ttccggcggt gtttcagtgg
     7501 gtgaagcgga ttacaccaaa accattcttg aagagctggg ggagatcgcc ttctggaagc
     7561 tggcgattaa accgggtaaa ccgttcgcgt tcggtaaact ttctaatagc tggttctgcg
     7621 gcctgccggg caacccggtt tcagcgacgc tgaccttcta tcaactggta cagcctttgc
     7681 tggcaaaact aagcggtaac accgccagcg gcctgcccgc gcgccagcgc gtacgcacag
     7741 cgtcccgcct gaaaaaaacg ccaggtcgcc ttgatttcca gcgcggcgtg ctgcaacgca
     7801 acgccgatgg cgaactggaa gtgacgacca ccggacatca gggttcacat atatttagct
     7861 cctttagcct cggcaactgc tttatcgtgc tggaacgcga tcgcggcaat gtggacgtgg
     7921 gcgaatgggt ggaagtagaa ccatttaacg cgttgttcgg aggcctgtaa tggcggaact
     7981 cagcgatcag gagatgctgc gctacaaccg gcaaatcatt ctgcgcggtt ttgattttga
     8041 cggtcaggag gcgctaaaag attctcgtgt gctggtcgtc ggcctcggtg gactcggctg
     8101 cgcagcctcg cagtatctgg caagcgccgg tgtcggcaac ctgacgctgc tcgacttcga
     8161 cacggtttcg ctctcgaatc tgcaacgcca gacgctgcac agtgatgcca cggtcgggca
     8221 acctaaggtt gaatccgcac gtgacgccct ggcgcggatc aacccgcata ttgcgattac
     8281 gccagtcaat gcactgctgg atgacgctga acttgctgcg atgattgctg aacacgatct
     8341 ggtgctcgac tgtacggata acgttgcggt acgtaatcaa ctgaacgcag gctgttttgc
     8401 cgcgaaggta ccgctggttt ccggcgcggc aattcgtatg gaaggtcaaa tcacggtctt
     8461 cacttatcag gacggtgaac catgctaccg ctgcctcagc cgtttgtttg gtgaaaatgc
     8521 attaacctgc gtggaagcag gcgtaatggc accgttgatt ggcgtgattg gttcgttgca
     8581 ggcaatggaa gcgatcaagc tgctggcagg ttatggcaaa ccggccagcg ggaaaatcgt
     8641 catgtacgat gcgatgacct gtcagtttcg cgaaatgaaa ctgatgcgca atccggggtg
     8701 tgaggtgtgc gggcagtaat tgccacctcc aggcctgata agacgcggca ggcatcggtg
     8761 cactccgcca cgtcatcagc aatttatgag gatgcagaac gtccggttaa atcgacgttc
     8821 tgccaaacgc tcccagccag tcctgttcaa acttcgccac agcggcttcc accgccggat
     8881 agctaatcat ctgttgtgcc acatccagtg gcagcgtaat tgattcacat cctgccagta
     8941 agcagtccag cgcctgacgc ggggttttga aactcgctgc cagcactttc gcctgcggcg
     9001 catgcatttt caataactgg tgtaagtcgg tcacagtctg aatgccgcta ccgccctgag
     9061 catcaatacg attaacgtaa ggcgcaacat attccgcacc tgccagcgcc gacagcagcc
     9121 cttgtgctgc gccatatacc gcggttccca gcgttggaat cccttccgct tttaacatct
     9181 taatagctgc cagcccctcg gcggtcaccg gaactttcac cacgatatcc gcaataatag
     9241 aacgcaattt acgtgcgtca ttcaccatcc cttcagcagt ggtagccatt acctgggcaa
     9301 acagacgccc ctgaccgccc atcgcttcat gaagttccgg aagcacaacc tccagcggtt
     9361 ttttacccgc ggcgataatg cttgggttag tggtcacacc cgccagcgga aaaatacgtg
     9421 acagcgcctt caccgcaaca acgtctgaag tatccagata cagttccata accatcctca
     9481 aaatgtactt tttgtctgaa tgtagactat cacgggaaaa ctctctgaac agttgacgca
     9541 catcaatcta actttcattc gaaagtattt taatgtttga acgaaacaga gaggcaacta
     9601 tgattttcaa tattcagcgc tactcgaccc atgatggccc cggtatccgc acggtcgtat
     9661 ttcttaaagg ctgttcgctg ggctgccgct ggtgtcagaa cccggaaagc cgcgcccgca
     9721 cgcaggatct gctgtatgac gcacgactgt gtctggaagg ctgcgagctg tgcgctaagg
     9781 cagcgccgga agtgattgag cgcgcgctga atggtttgct tattcatcgg gaaaagttaa
     9841 ccccggagca ttttacggcg ctaactgact gctgcccgac acaggcgtta accgtgtgtg
     9901 gggaagtgaa aagcgttgag gagatcatga cgaccgttct gcgcgataaa ccgttttacg
     9961 atcgcagcgg cggcggctta acgctttctg gcggtgaacc gtttatgcag ccggaaatgg
    10021 cgacggcgct gctgcaagcc agccacgagg ccggcattca tactgcggta gaaacctgtc
    10081 tgcatgtgcc gtggaaatat atcgcccctt ctctgcccta tatcgatctg tttcttgccg
    10141 atttaaaaca cgttgccgac gcgccgttta aacagtggac cgacggtaac gccgccagag
    10201 tgctggataa cctgaaaaaa ctcgctgcag cgggcaaaaa aatcattatc cgcgtgccgc
    10261 tgattcaggg ctttaatgcc gacgaaacct ctgtgaaagc cattaccgat tttgccgccg
    10321 acgagctgca cgttggtgaa attcatttcc tgccctacca cacgctgggc atcaacaaat
    10381 atcacttact taatctaccc tatgacgccc cggaaaaacc gcttgatgcg ccagaactgc
    10441 tcgactttgc ccagcagtat gcctgccaga aagggttaac cgcgacctta cgaggataac
    10501 aaccatgacc acactgaaac tggacacgct cagcgaccgc attaaagcgc acaaaaatgc
    10561 gctggtgcat attgtgaaac cgccagtctg taccgagcgc gcgcagcact ataccgagat
    10621 gtatcaacaa catctcgata agccgatccc ggtacgtcgc gcgctggcgc tggcacatca
    10681 cctggcgaat cgcactatct ggatcaagca cgatgagttg atcatcggca accaggcaag
    10741 cgaagttcgc gccgcgccga tcttcccgga atatactgtc tcctggatcg aaaaagagat
    10801 tgatgatctg gcggatcgtc ccggtgctgg ttttgcggtg agcgaagaga acaaacgcgt
    10861 tctgcatgaa ttgtgcccgt ggtggcgcgg tcagaccgta caggatcgct gctacggcat
    10921 gtttaccgat gagcaaaaag gtctgctggc gaccggcatc attaaagcgg aaggcaatat
    10981 gacctccggc gatgcgcacc tggcggtgaa tttcccgctg ctgctggaaa aaggtcttga
    11041 tggtctgcgc gagaaagtag cggaacgtcg ctcgcgcatc aacctgacgg tgctggaaga
    11101 tttacacggt gagcaattcc tgaaagcgat tgatatcgtg ctggtggcag tcagtgaaca
    11161 cattgaacgt ttcgctgccc tggcgcgtga aatggccgcg accgaaaccc gcgaaagccg
    11221 tcgcgatgaa ctgctgacga tagcagaaaa ctgcgatctt atcgcccacc agccgccgca
    11281 gactttctgg caggcgctgc aactgtgtta cttcatccag ttgattttgc agatcgaatc
    11341 taacggtcac tcggtatcgt ttggtcgtat ggaccagtat ctctacccat actatcgccg
    11401 cgacgttgaa ctcaaccaga cactggatcg cgaacacgcc atcgaattgc tgcatagctg
    11461 ctggctgaaa ctgctggaag tgaacaagat ccgctccggc tcgcactcga aagcctctgc
    11521 gggaagtccg ctgtatcaga acgtcaccat tggcgggcaa aatctggttg atggtcaacc
    11581 aatggacgcg gtgaatcccc tctcttacgc gatccttgaa tcctgcggtc gcctgcgttc
    11641 cactcagcct aacctcagcg tgcgttacca tgcaggaatg agtaacgatt tcctcgacgc
    11701 ctgcgtacag gtgatccgct gcggcttcgg gatgccggcg ttcaacaacg acgaaatcgt
    11761 gatcccggaa tttattaaac tcggtattga accgcaggac gcttacgact acgcagcgat
    11821 tggttgtatc gaaaccgccg tcggtggcaa atggggctat cgctgtaccg gcatgagctt
    11881 tatcaacttc gcccgcgtga tgctggcggc gctggaaggc gggcgtgatg ccaccagcgg
    11941 caaagtgttc ctgccacaag aaaaagcgtt gtcggcaggt aactttaaca atttcgatga
    12001 agtaatggac gcgtgggata cgcaaatccg ttactacacc cgcaaatcaa tcgaaatcga
    12061 atatgtcgtc gacaccatgc tggaagagaa cgtgcacgat attctctgct cggcgctggt
    12121 ggatgactgt attgagcgag cgaaaagtat caagcaaggc ggcgcgaaat atgactgggt
    12181 ttctggcctg caggttggca ttgccaacct cggcaacagc ctggcggcag tgaagaaact
    12241 ggtatttgaa cagggcgcga ttggtcagca acagctggct gccgcactgg cggatgactt
    12301 cgatggcctg actcatgagc aactgcgtca gcggctgatt aacggtgcgc cgaagtacgg
    12361 caacgacgat gataccgtcg atacgctgct ggctcgcgct tatcagacct atatcgacga
    12421 actgaaacag taccataacc cacgctacgg tcgtggtccg gttggcggca actattacgc
    12481 gggtacgtcg tctatctccg ctaacgtacc gtttggcgcg cagacgatgg caacgccgga
    12541 cggacgtaaa gcgcataccc cgctggcaga aggcgcaagc ccggcttccg gtactgatca
    12601 tcttggccct actgcggtca ttggctcggt gggtaaactg cctacggcag cgattctcgg
    12661 cggggtgttg ctcaaccaga aactgaatcc agcaacactg gagaacgaat ctgacaagca
    12721 gaaactgatg atcctgctgc gcaccttctt tgaagtgcat aaaggctggc atattcagta
    12781 caacatcgtt tcccgcgaaa cgctgctgga agcgaaaaaa catccggatc agtaccgcga
    12841 tctggtagtg cgtgtcgcgg gctattccgc cttcttcacc gcgctctctc cagacgcaca
    12901 ggacgatatc atcgcccgta ctgaacatat gctgtaatct tctcagcccg gcggcgtcgt
    12961 cgccgggcta aatcattcac atcatcaatt tcatccttac tttcattcga aatataattt
    13021 gtgctctgcg tcacattatt attacactgt tacggtttca ttttcgagcc aggagcacac
    13081 ctatgagcgt aaaagttatc gtcacagaca tggacggtac ttttcttaac gacgccaaaa
    13141 cgtacaacca accacgtttt atggcgcaat atcaggaact gaaaaagcgc ggcattaagt
    13201 tcgttgtcgc cagcggtaat cagtattacc agcttatttc gttctttcct gagctaaagg
    13261 atgagatctc ttttgtcgcg gaaaacggcg cactggttta cgaacatggc aatcaactgt
    13321 tccacggcga actgacccga catgaatcgc ggattgttat tggcgagttg ctaaaagata
    13381 agcaactcaa ttttgtcgcc tgcggtctgc aaagtgcata tgtcagcgaa aacgcccccg
    13441 aagcattcgt cgcactgatg gcaaaacact accatcgcct gaaacctgta aaagattatc
    13501 aggagattga cgacgtactg ttcaaatttt cgctcaacct gccggatgaa caaatcccgt
    13561 tagtgatcga caaactgcac gtagcgctgg atggcattat gaaacccgtt accagtggtt
    13621 ttggctttat cgacctgatt attcccggtc tacataaagc aaacggtatt tcgcggttac
    13681 tgaaacgctg ggatctgtca ccgcaaaatg tggtagcgat tggcgacagc ggtaacgatg
    13741 cggagatgct gaaaatggcg cgttattcct ttgcgatggg caatgctgcg gaaaacatta
    13801 aacaaatcgc tcgttacgct accgatgata ataatcatga aggcgcgctt aatgtgattc
    13861 aggcggtgct ggataacaca tcccctttta acagctgacc tctctaccgg agccatgcgc
    13921 tccggttttc ccttcttttt acatcctgta catcacatca aatcattaaa cttgcattaa
    13981 cttatttata acttatattg tcacatgcaa atcatcatag tttcgaatga aagttaatgc
    14041 gaggtggtta tggcttctac ttttaccagc gacacattgc ctgccgatca caaagcagct
    14101 atccgtcaga tgaagcacgc gctgcgggcg cagcttggcg acgtccagca gatctttaat
    14161 cagctaagcg atgacattgc cacgcgagtg gctgaaatca acgcactcaa agcacagggc
    14221 gatgccgtct ggccggtgct gtcttatgcc gatatcaaag caggtcatgt tactgcagag
    14281 cagcgcgaac agattaaacg tcgcggttgt gcggtgataa aaggccattt cccccgcgaa
    14341 caagcgctag gctgggatca gtcgatgctg gactatctgg accgcaaccg ctttgacgag
    14401 gtctacaaag gccccggcga taatttcttc gggacgctca gcgcttcacg tcccgagatt
    14461 taccccatct actggtcgca ggcgcaaatg caggctcgcc agagtgaaga aatggcgaat
    14521 gcgcagtcgt ttctcaatcg tctgtggaca tttgaaagtg atggaaagca atggtttaac
    14581 ccggatgtga gcgtcatcta ccctgaccgt atccgccgcc gtccgcccgg aacgacctcc
    14641 aaaggtcttg gagcgcatac cgactccggg gcactggaac gctggctgct tccagcgtat
    14701 cagcgcgttt tcgccaacgt ctttaatggc aatctggcgc aatatgatcc ctggcatgcg
    14761 gcacatcgta cggaagttga agagtacacg gtggacaaca ccaccaaatg ttccgtgttt
    14821 cggacattcc agggctggac agcgctctct gatatgctgc ctggtcaggg gctgctgcac
    14881 gtcgtgccca ttcctgaagc catggcgtac gtactgttac gtccgctgct tgatgatgtg
    14941 ccggaggatg aactgtgcgg cgtagcgccc ggaagagtat tgccggtatc agagcaatgg
    15001 catccactgt tgattgaggc gttaaccagc attccaaaac tcgaagccgg agactccgtc
    15061 tggtggcact gcgacgtcat ccattccgtt gcccccgttg aaaatcaaca gggttggggc
    15121 aacgtgatgt acattcctgc ggcaccgatg tgcgagaaaa atcttgccta cgcgcacaag
    15181 gtgaaggccg cactggaaaa aggcgcatcg ccgggcgact tcccgcgcga ggactatgaa
    15241 acaaactggg aaggacgctt tacgcttgcc gacctcaaca ttcacggtaa gcgagcgttg
    15301 ggcatggatg tttgagagtt aatggaagtg aattgatgtg ggattaagaa aatcgattcg
    15361 acgttactca gattgctgac aagtgcgctt tcttaatgcc tgatgcggcg ttaacgcctt
    15421 atctggccta caaagtcctg cagattcaat atattacagg tact
//
LOCUS       DAPHDW010000103        15365 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.104, whole genome shotgun sequence.
ACCESSION   DAPHDW010000103 DAPHDW010000000
VERSION     DAPHDW010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15365)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15365)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15365
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.104"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            330..1961
                     /gene="oppA"
                     /locus_tag="RH057_003207"
     CDS             330..1961
                     /gene="oppA"
                     /locus_tag="RH057_003207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HDV3129538.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLLYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2047..2967
                     /gene="oppB"
                     /locus_tag="RH057_003208"
     CDS             2047..2967
                     /gene="oppB"
                     /locus_tag="RH057_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HDV3129539.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            2982..3890
                     /gene="oppC"
                     /locus_tag="RH057_003209"
     CDS             2982..3890
                     /gene="oppC"
                     /locus_tag="RH057_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HDV3129540.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTSGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3902..4915
                     /gene="oppD"
                     /locus_tag="RH057_003210"
     CDS             3902..4915
                     /gene="oppD"
                     /locus_tag="RH057_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HDV3129541.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPEHELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4912..5916
                     /gene="oppF"
                     /locus_tag="RH057_003211"
     CDS             4912..5916
                     /gene="oppF"
                     /locus_tag="RH057_003211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HDV3129542.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(5969..6298)
                     /locus_tag="RH057_003212"
     CDS             complement(5969..6298)
                     /locus_tag="RH057_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HDV3129543.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEINDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6333..7793)
                     /gene="cls"
                     /locus_tag="RH057_003213"
     CDS             complement(6333..7793)
                     /gene="cls"
                     /locus_tag="RH057_003213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HDV3129544.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAR
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7936..8109
                     /locus_tag="RH057_003214"
     CDS             7936..8109
                     /locus_tag="RH057_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HDV3129545.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8164..9417)
                     /gene="kch"
                     /locus_tag="RH057_003215"
     CDS             complement(8164..9417)
                     /gene="kch"
                     /locus_tag="RH057_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HDV3129546.1"
                     /translation="MSHWATFKQTATNLWVTLRHDILALAVFLNGLLIFKTIYGMSVN
                     LLDIFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAISIILLLIALIY
                     TLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTTLLFYSTYGALY
                     LSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVIISGITVFATSMT
                     SIFGPLIRGGFNKLVKGNNHTMHRKDHFIVCGHSILAINTILQLNQRGQNVTVISNLP
                     EDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDADNAFVVLSAKD
                     MSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEEINNDMLVSMLL
                     NSGHGIFSDNDEQETKADSKESVQK"
     gene            complement(9716..10012)
                     /locus_tag="RH057_003216"
     CDS             complement(9716..10012)
                     /locus_tag="RH057_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415767.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HDV3129547.1"
                     /translation="MLYVIYAQDKADSLEKRLSVRPAHLARLQLLHDEGRLLTAGPMP
                     AVDSNDPGAAGFTGSTVIAEFESLEAAQAWADADPYVAAGVYEHVSVKPFKKVF"
     gene            10236..10955
                     /gene="tonB"
                     /locus_tag="RH057_003217"
     CDS             10236..10955
                     /gene="tonB"
                     /locus_tag="RH057_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HDV3129548.1"
                     /translation="MTLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAPAQP
                     ISVTMVAPADLEPPQAVQLPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPKPKP
                     VKKVQEQPKRDIKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALSRNQ
                     PQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRYEPG
                     KPGSGIVVNILFKINGTTEIQ"
     gene            complement(10995..11393)
                     /gene="yciA"
                     /locus_tag="RH057_003218"
     CDS             complement(10995..11393)
                     /gene="yciA"
                     /locus_tag="RH057_003218"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HDV3129549.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(11498..12037)
                     /locus_tag="RH057_003219"
     CDS             complement(11498..12037)
                     /locus_tag="RH057_003219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HDV3129550.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLGKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            complement(12067..12810)
                     /locus_tag="RH057_003220"
     CDS             complement(12067..12810)
                     /locus_tag="RH057_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HDV3129551.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSGLIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            complement(12838..12927)
                     /locus_tag="RH057_003221"
                     /pseudo
     CDS             complement(12838..12927)
                     /locus_tag="RH057_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            13167..13805
                     /gene="ompW"
                     /locus_tag="RH057_003222"
     CDS             13167..13805
                     /gene="ompW"
                     /locus_tag="RH057_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HDV3129552.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            complement(13865..14371)
                     /locus_tag="RH057_003223"
     CDS             complement(13865..14371)
                     /locus_tag="RH057_003223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV3129553.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSISGYV
                     FEQFEIACYTSLLAAAKNAGDTASIPTIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(14417..14917)
                     /locus_tag="RH057_003224"
     CDS             complement(14417..14917)
                     /locus_tag="RH057_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HDV3129554.1"
                     /translation="MNMKTIEDVFIHLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            complement(15003..15182)
                     /locus_tag="RH057_003225"
     CDS             complement(15003..15182)
                     /locus_tag="RH057_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HDV3129555.1"
                     /translation="MAEHRGGSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
BASE COUNT         4087 a         3593 c         3712 g         3973 t
ORIGIN      
        1 attgcttaac aattttgcaa aatgtattgg cgagtaagaa ccgcatttgg tactttccgg
       61 gcaaccgcca gacgattatt tattggtaat gagaataatt aacaattaaa gagcgtcgcg
      121 aaagaataat gtgtctcgac aggggagaca cagtacgaat cgacataagg tgatcgtctg
      181 aatcaccaga ataaataaag tcggtgatag taatacgtaa cgataaagta acctgacagc
      241 agaaagtctc cgagcctgtg cagggtccca atccgggatt acacatgctg gttaatacca
      301 gtaattataa tgagggagtc caaaaaacaa tgaccaacat caccaagaga agtttagtag
      361 cagctggcgt tctggctgcg ctaatggcag ggaatgtcgc gctggcagct gatgtacccg
      421 caggcgtcac actggcggaa aaacaaacac tggtacgtaa caatggttca gaagttcagt
      481 cattagatcc gcacaaaatt gaaggtgttc cggagtctaa tatcagccga gacctgtttg
      541 aaggcttact ggtcagcgat cttgacggtc atccagcacc tggcgtcgct gaatcctggg
      601 ataataaaga cgcgaaagtc tggaccttcc atttgcgtaa agatgcgaaa tggtctgatg
      661 gcacgccagt cacagcacaa gactttgtgt atagctggca acgttctgtt gatccgaaca
      721 ctgcttctcc gtatgccagt tatctgcaat atgggcatat cgccggtatt gatgaaattc
      781 ttgaagggaa aaaaccgatt accgatctcg gcgtgaaagc tattgatgat cacacattag
      841 aagtcacctt aagtgaaccc gttccgtact tctataaatt acttgttcac ccatcaactt
      901 caccggtgcc aaaagccgct atcgagaaat tcggcgaaaa atggacccag cctggtaata
      961 tcgtcaccaa cggtgcctat accttaaaag attgggtcgt aaacgaacga atcgttcttg
     1021 aacgcagccc gacctactgg aacaacgcga aaaccgttat taaccaggta acctatttgc
     1081 ctattgcttc tgaagttacc gatgtcaacc gctaccgtag tggtgaaatc gacatgactt
     1141 ataacaacat gccgatcgaa ttgttccaga agctgaaaaa agagatcccg gacgaagttc
     1201 acgttgatcc atacctgtgc acttactatt acgaaattaa caaccagaaa ccgccattca
     1261 acgatgtgcg tgtgcgtacc gcactgaaac taggtatgga ccgcgatatc attgttaata
     1321 aagtgaaagc gcagggcgac atgcccgcct atggttacac tccaccgtat actgatggcg
     1381 caaaattgac tcagccggaa tggtttggct ggagccagga aaaacgtaac gaagaagcga
     1441 aaaaactgct ggctgaagcg ggttataccg cagacaaacc gttgaccatc aacctgttgt
     1501 ataacacctc cgatctgcat aaaaagctgg cgattgctgc ctcttcattg tggaagaaaa
     1561 acattggtgt aaacgtcaaa ctggttaacc aggagtggaa aacgttcctc gacacccgtc
     1621 accagggtac ttttgatgtg gcccgtgcag gctggtgtgc tgactacaac gaaccaactt
     1681 ccttcctgaa caccatgctt tcgaacagct cgatgaatac cgcgcattat aagagcccgg
     1741 cctttgacag cattatggcg gaaacgctga aagtgactga cgaggcgcag cgcacagctc
     1801 tgtacactaa agcagaacaa cagctggata aggattcggc cattgttcct gtttattact
     1861 acgtgaatgc gcgtctggtg aaaccgtggg ttggtggcta taccggcaaa gatccgctgg
     1921 ataataccta tacccggaat atgtacattg tgaagcacta atggcaatac gtggggcagg
     1981 agtgtcctgc tccacggtgt ctgattttta tcgcattaca gaaggcacag gccagaaggt
     2041 agggcaatgt taaaatttat tctacgtcgc tgtctggaag cgattccgac gctatttatt
     2101 cttattacta tttcgttctt tatgatgcgc ctcgcgccgg gaagcccttt taccggcgaa
     2161 cgtactttac cgccagaagt gatggccaat atcgaagcga aatatcatct taatgatccg
     2221 atcatgacac agtatttcag ctacctgaaa caactggcgc acggtgattt cggtccatcg
     2281 tttaaatata aagattattc ggtcaatgac ctggttgcat ccagttttcc cgtttctgcc
     2341 aaactgggag ccgcagcatt tttccttgcg gtaatactgg gtgttagtgc tggcgttatt
     2401 gccgcattaa aacaaaacac caaatgggac tataccgtga tggggctggc aatgaccggg
     2461 gttgttatcc ccagttttgt ggttgcgcca ttattagtca tgatatttgc gatcattttg
     2521 cattggctgc cgggcggtgg ctggaatggt ggggctctta aattcatgat attgccgatg
     2581 gtggcgttgt cactcgctta tatcgccagt attgcgcgta ttacccgtgg ctctatgatt
     2641 gaagtattac actccaactt tattcgtact gcccgggcga aagggttacc tatgcggcgg
     2701 atcattttac gccacgcatt aaaacctgct ctgttacccg tgctctccta tatgggcccc
     2761 gcatttgtcg gcattattac cggttctatg gtcatcgaaa ccatttatgg tttgccgggg
     2821 attgggcaac tgttcgttaa tggcgcattg aaccgtgact attccttagt gttaagcctg
     2881 accatcctgg ttggtgcttt aaccattttg tttaatgcca ttgtcgatgt gctatatgcg
     2941 gttatcgacc cgaaaatccg ttactgatac tggagctcgc gatgatgtta agtaagaaaa
     3001 acagcgagac gctggaaaat ttcagtgaaa agctggaggt cgaagggcgc agcttgtggc
     3061 aggacgcacg tcgacgtttt atgcataacc gtgcggcggt tgccagtctg atagtgctgg
     3121 tgctgatcgc gttatttgta atcctggcac cgatgctttc gcagtttgcc tatgacgata
     3181 ctgactgggc gatgatgtcc agcgccccgg atatggagtc cggtcactac tttggtactg
     3241 actcatccgg tcgcgacctg cttgtgcgcg ttgcgattgg cgggcgtatc tcactcatgg
     3301 tcggtgttgc tgcggcactg gtggcagtgg tcgtggggac actttacggt tcgctttccg
     3361 gttatctggg cggtaaagtg gattcggtaa tgatgcgtct gctggaaatc ctcaactcct
     3421 tcccattcat gttcttcgtc attttgcttg tgaccttttt cggtcaaaac atcctgctga
     3481 ttttcgtggc gattggcatg gtttcctggc tggatatggc tcgtattgtg cgtgggcaaa
     3541 ccctgagtct gaagcgcaaa gagtttattg aggcggcaca agttggcggt gtatcgacat
     3601 cgggcattgt tattcgccac attgtgccga atgtactcgg tgtggtggtg gtctacgcat
     3661 cacttctggt gcccagcatg atcctctttg aatctttcct tagcttcctg gggttgggta
     3721 cgcaagagcc gttaagcagc tggggtgcat tgctgagtga tggcgcgaac tcgatggaag
     3781 tctctccatg gttattgttg ttcccagcgg gattcctcgt ggtgacgctg ttttgtttca
     3841 actttatcgg cgatggcttg cgtgatgccc tcgacccgaa agatcgttaa ggagtgcagc
     3901 catgagcgta attgaaactg caactgtgcc gctcgcacaa caacaggctg acgcactgct
     3961 gaacgtgaaa gatttgcgtg tcacctttag taccccggac ggcgacgtca cggcggtcaa
     4021 tgatttgaat ttttccctac gtgccggaga aacgctgggt attgtaggtg agtctggttc
     4081 gggtaaatcg caaactgcat ttgcgttgat gggcctgttg gctgccaacg gacgtattgg
     4141 cggatcggca accttcaatg ggcgtgaaat cctcaatttg ccagagcatg aactcaataa
     4201 actgcgcgct gaacaaatct caatgatttt tcaggaccca atgacttcgt tgaatcccta
     4261 tatgcgcgtc ggtgagcagt taatggaagt gctgatgctg cataagaaca tgagcaaagc
     4321 tgaggcgttt gaagagtcgg tgcggatgct cgatgcggta aaaatgccgg aagcgcgtaa
     4381 acgcatgaaa atgtacccgc acgaattttc tggcggcatg cgtcagcgag tcatgattgc
     4441 gatggcattg ctatgtcgac ctaagctact gattgcggat gaacccacta ctgcgctgga
     4501 cgtcaccgta caggcgcaga tcatgacgct attaaatgaa ctgaagcggg aatttaatac
     4561 cgccatcatt atgatcaccc acgatcttgg cgtggtggcg gggatctgtg acaaagtgct
     4621 ggtaatgtac gccgggcgca cgatggaata tggcaatgcg cgcgatgtct tttatcaacc
     4681 cgttcatcct tattctatcg gtttgctcaa cgcggtgccg cgtcttgatg cggaaggtga
     4741 aacaatgttg accatcccgg gtaatccgcc aaacttgctg cgattaccga aaggttgccc
     4801 gttccagcca cgttgtccac atgcgatgga aatttgtagt agcgcaccgc cgctggaaga
     4861 gtttacgcct ggccgtctgc gtgcttgctt taaaccggtg gaggaactgt tatgaatgct
     4921 gtaactgaag gaagaaaagt cctccttgaa atcgccgatc ttaaagtgca ctttgaaatc
     4981 aaagatggca aacagtggtt ctggcaaccg ccgaaaacgc tcaaagccgt cgatggtgtc
     5041 actcttcgcc tgtatgaagg ggaaacatta ggtgtggtag gggaatcggg atgcggtaag
     5101 tccacctttg ctcgcgccat catcggtttg gtcaaggcga ccgacggtca tgttgcctgg
     5161 ttaggtaaag agttgctggg catgaagccc gatgaatggc gtgccgttcg cagtgatatt
     5221 cagatgattt tccaggatcc gttggcatcg ctaaacccgc gtatgaccat cggcgagatc
     5281 atcgctgaac cactgcgtac ttatcatccg aaaatgtcac gccaggaagt tcgcgagcgc
     5341 gtgaaggcga tgatgctgaa agtcgggtta ttgcctaacc tgattaaccg ctatccgcat
     5401 gagttctccg gtgggcagtg ccagcgtatc gggattgctc gtgctcttat tcttgaaccg
     5461 aagctgatta tctgcgatga gccggtttcg gcgctggacg tgtcaattca ggcgcaggtg
     5521 gtcaacctgc tccagcagct gcaacgtgag atgggattgt cattaatttt tatcgctcat
     5581 gacctggccg tggtaaaaca catttccgat cgtgtgttgg tgatgtatct cggccatgcg
     5641 gtagaactgg ggacctatga tgaggtctac cacaatccac tacatcctta caccagggca
     5701 ttgatgtcgg cagtccccat acctgatccg gatctggaga agaacaaaac catccagtta
     5761 ctggaagggg aattaccgtc gccgatcaac ccgccttccg gttgtgtttt ccgtacccgt
     5821 tgcccgattg ccggtccgga gtgcgccaaa acacgtcctg ttctggaggg gagtttcaga
     5881 cacgccgttt cttgcctgaa agtcgatccg ctttaaacac aataagggct gacagttgtc
     5941 agcccttttt cacgccgaaa gcgattattt attcccgcca gatgatatgg caaagtttgt
     6001 gatctttttc gcggcataac agaatgcggg cgaaaacatc gttgatttca ccatcttcac
     6061 tgtccgccag accaatcacc acttcggcaa aaaagtctgg gttcaaatcg aaatcaacat
     6121 gttcctgcca gtcttccgcc ggatcgaata actccgcgcc gccgcgctct tcaaactgaa
     6181 gattgaacag cagaacatca gctggatcga gattgtcggc agccagttcg agaaaaatat
     6241 cgtaggcctg ctcaagcgtt tcatcttcag tcaggcgatt gtttagatcc atatccatag
     6301 tcactacctg tttaacctct gttggcgacg ttttacagca acggactgaa gaagtaaaac
     6361 agtcgctcgg cgacacgttg ccataatgga cgttttagcc ataaacgggc atcgagcaga
     6421 cgtgaacgcg aaatataatc gtcctgaacg gcggcaaggt ctgcaccaaa acctttatcg
     6481 tcgattgcca gggtaatctc gaaattcagc cacagactac gcatatcaag gttaacggtg
     6541 ccaaccagac ttagttcgcc atcgaccagc acgctcttgg tatgcagtaa cccgccttca
     6601 aactgataaa ttttaacccc agcagcaaga agttccgtaa agaatgcgcg actggcccag
     6661 ccgaccagca tcgagtcatt ttttcgcgga aggataatac tgacatccac cccgcgctgc
     6721 gccgccgtgc aaatcgcatg aagtaaatca tcgcttggca caaagtaggg cgtggtcatg
     6781 atcaaatatt cacgcgccga ataagccgca gtcaataatg cctggtgaat gagatcttcc
     6841 ggaaagccgg ggccagaagc aattgtgtga atggtgtgac cgctggcctg ttcaaacggc
     6901 ataatattga catctggtgg tggcggcaga atacgttttc cggtttcaat ctcccagtcg
     6961 caggaataaa taatccccat cgcggtggcg atggggcctt ccatacgcgc catcagatca
     7021 atccattgcc ctacgcccgc atcttgtttg aagtagcgag gatcgaccat attcatgctg
     7081 ccggtgtacg cgatgtaatt atcgatcatg atcatcttgc gatgttggcg caggtccata
     7141 cggcgtaaaa acacacgcat cagattgacc tttaaggctt cgaccacttc aataccggca
     7201 ttacgcatta gctcgggcca cgggctgcgg aaaaaagcca cactcccggc ggagtcgagc
     7261 atcaatcggc aatgaatacc acgccgtgca gccgccatta atgattcagc cacctggtcc
     7321 gccatgccgc cgggctgcca gatataaaac accatctcaa tattatggcg cgcgagctgg
     7381 atgtcgcgga ttaacgcctg catcacatca tctgactcgg tcatcagttg tagctgattc
     7441 cctttgaccc cagcgatccc ctgacgacgc tcgcaaagct tgaataatgg cgcagcgaca
     7501 ctgctatttt cttcggcgaa gatatgctta caggctttaa ggtcgttaag ccattttgcg
     7561 gtggaaggcc acatcgctct ggcgcgctca gcgcggcgtt tgcctaaatg gagctcgcca
     7621 acggcaagat aggcaataat tccgactaac ggcagaatgt aaataatcaa cagccaggcc
     7681 atcgcggagg gaactgcgcg tcgtttcatt agaatgcgta aagttacgcc tgcaatgagc
     7741 aaccagtatc ccagaatggc caaccaactc accaacgtat aaacggttgt catagattag
     7801 aaatcctttt gaaagcgcat tgttatgagt ttacgcatat ggattcatct ggcaaataaa
     7861 aacgcgggaa aagcactggt cagcgtcggg ggtgggggta taatgaccat tctgttattg
     7921 catagagtag ttaacatgaa gcggagtaga acggaagtgg ggcgctggcg catgcagcgt
     7981 caggctagcc gacgtaaatc gcgttggctt gaggggcaat cgcgccgaaa tatgcgtatc
     8041 cacagcatca ggaagtgcat tctaaacaaa cagcgtaact cgttattgtt tgcgatctac
     8101 aatatctaaa tatactgggc accattgcgg tgcccaataa gtttactctt tcgtctttaa
     8161 actctatttt tgcactgatt ctttactgtc agctttcgtt tcttgttcat cgttatcgct
     8221 gaaaatgcca tgaccggagt tcaacaacat tgaaacgagc atatcattat taatctcttc
     8281 accatttaaa actcgcgcca gaatttcgct gccaaacagt tgcggtgaga gaatgatatc
     8341 cggatgtacc atcttaatct tattcaggtt tttgctatca ctgacggcga gaactgtttt
     8401 gacatcactg ctcatatctt ttgccgagag tacaacaaac gcgttatctg catcgttatc
     8461 actcagcgcc agaatggctc ggcagcgatc gattcccgct ttctttaata ctgaactgtc
     8521 attactgtca ccggggataa catcagcgtt atcgcctaag cgttgctcaa gttgcttgat
     8581 atcatcttca ggcaagttgc tgataaccgt tacgttttgt ccgcgttgat tcagttgcag
     8641 gatcgtattg atggcgagaa tcgaatgtcc gcaaacaata aaatgatctt tacgatgcat
     8701 tgtatgattg tttcctttta caagtttgtt gaatcccccg cggataagcg gaccaaaaat
     8761 tgaggtcata gatgtggcaa aaacggtaat gccggaaata atgaccgaaa tagtgaacaa
     8821 tcgtgctgat tcagaaacag ggacaatatc gccatagccg acggttgaca tggtttctat
     8881 cgaaaaatag aacgcggtca tcaaactttc tattcgcgga ttaaaacctt cgcttaaata
     8941 aagcgcaccg taggttgagt aaaacagtaa tgtcgtgaaa ctaataaatg caaaaattgt
     9001 cccggctgcg gcgctactgt gggagaagtc tttgcgcagt atcagcaaaa acaccagcgt
     9061 aaaaatgcaa aatccaatac taaatttcag ccagggataa aaatgcaggg tgtaaattag
     9121 cgctatcaac aacaaaatga tactgattgc ccatgcgagc tttgcccgga acagtaagcc
     9181 aatggagttc aggacaagaa agacgccgag cataaatagt ggggcgtttg ccagcaagga
     9241 gagatccaac tctgaaaatg ctttgatatg gaaaatatca agcaaattga ccgacatacc
     9301 atagattgtt ttaaaaataa gcaatccatt taaaaagacg gccagcgcga gaatgtcgtg
     9361 ccgtagggtc acccataaat ttgttgctgt ttgtttgaat gtagcccagt gactcactga
     9421 tacctctgta accacaattt tgtttaatta tcttagcgtg cacattttct gaacatacat
     9481 gcagcgcgtg aatgtgttaa acaatagtag atgactttat cggcagtaat caaaggtttt
     9541 gagcgagcaa ctctgcgtca ggaaagggca ttttcctgaa tatgctcaca tcatataaag
     9601 aaatacagat aaagttatta tctgcttgtg gtagtgaatg cactgaccgg ctataaggaa
     9661 aggccaaaca agaacacggt tgcaaaaacc gtgcccttaa atattgaatc tctattcaga
     9721 acactttctt aaatggtttc actgaaacgt gttcatagac tcctgccgct acgtacgggt
     9781 cagcatcggc ccaggcctga gctgcttcca gcgattcaaa ttcagcaata acggttgagc
     9841 cagtaaatcc cgcagcccct ggatcgttac tgtctaccgc tggcattgga ccagctgtca
     9901 acaaacgacc ttcatcatgc agtaactgta aacgtgctaa atgtgccgga cgaacggaaa
     9961 ggcgtttttc gaggctatca gctttgtctt gagcgtaaat aacatacaac acgggcacaa
    10021 ctccttgttc gggaaagttg taaagtacgt tatttgaaag ggcgaagatc tgcaacggaa
    10081 agatgatgtc tttgttaaga ccatgcataa attaaggtca attacgccaa aaatgacatt
    10141 ttcactgatt ctgatcgtct tgccttattg aatatgattg ctatttgcat ttaaaatcga
    10201 gacctggttt ttctactgaa atgattatga cttcaatgac ccttgattta cctcgccgct
    10261 tcccctggcc gacgttactt tcggtctgca ttcatggtgc tgttgtggcg ggtctgctct
    10321 atacctcggt acatcaggtt attgaactac ctgcgcctgc gcagccaatt tctgtcacga
    10381 tggttgcgcc tgctgatctc gaaccgccac aagccgttca gctgccaccg gagccggtgg
    10441 tagagccaga accggaacct gagccgatcc ccgaaccgcc aaaagaagca ccggtggtca
    10501 ttgaaaagcc gaagccgaaa cctaagccaa aaccgaagcc ggtgaaaaag gtacaggagc
    10561 agccaaaacg cgatatcaaa cccgtagagt cgcgtccggc atcaccgttt gaaaatacgg
    10621 cacccgcgcg ccccacatca agtacggcaa cggctgcaac cagcaagccg gttaccagtg
    10681 tggcttcagg accacgcgca ttaagccgta atcagccgca gtatccggca cgagcacagg
    10741 cattgcgcat tgaagggcag gttaaagtta aatttgacgt cacgccggat ggtcgcgtgg
    10801 ataacgtaca aatcctctca gccaagcctg cgaacatgtt tgagcgtgag gtgaaaaatg
    10861 cgatgcgcag atggcgttat gagccgggta agccaggcag tgggattgtg gtgaatatcc
    10921 tgtttaaaat taacggcacc accgaaattc agtaagcaga aagtcaaaag cctccgaccg
    10981 gaggcttttg actattactc aacaggtaag gcgcgaggtt ttccttcagg atcaaccgcg
    11041 acatacttaa ataatgcttc tgtcgcttta tagcgttgcc cgattggttc agacgctact
    11101 tttttcaccc acacttcaat attaatgctg accgatgtcg tccctttctg gacacagcgt
    11161 gcatagcagc acaccacatc gccgaccgca accggccgta agaaagtcat tccttcaacc
    11221 cgcacagtca ctacgcgacc gtgggcaatt tcttttgcca gaatagcgcc gccaatatcc
    11281 atttgtgaca ttaaccaacc accaaagatg tcaccattgg cattggtatc ggcgggcatg
    11341 gctaaagtac gtaaaacaag atcgccctga gggacgttat gtgttgtaga catggtaaaa
    11401 ccgacttaaa aggaaaatca caggcgcgat gctactatga ttttcctgag gagaacagag
    11461 gagatacagc gccagccccg aaggactggc agtctggtta ggatttatct tcctgcggca
    11521 tgtggcggta gatatagata ccgcttaaca atgtgaagat taaggtaagg gcggtcaggc
    11581 caaagacttt aaagttgacc caaatatttt gcggcagcca gaatgcgatg tagatgtttg
    11641 ccagaccgca aaggataaag aaaacagccc aggccagatt cagcttcgac cataccggtt
    11701 gcggcagcgt gagttcttta cccagcatcc gctgaattag cggctttttc atcacccatt
    11761 ggctgactaa cagggctccc gcaaatagag cataaatgac tgtaaccttc catttaataa
    11821 actcatcatt gtggaagaac agcgtcaagc caccgaagac gaccaccaga acaaaagtga
    11881 tcagggccat cttctcaacc ttacgaaagc gaacccagct atagataagc acaatcgccg
    11941 tggcgacgat cagcgccgca gtagccgcat agatgtcata aatcttgtaa aacgcgaaaa
    12001 agacaaccag cggtaaaaaa tcaagaaact gcttcatttt acgattccgt aatcaaatgc
    12061 ttaaggttat tggcgaatca acatatacag gcggaacaga tagatgagca atatggcaga
    12121 aatcaggttg ctcaaggtgt tcgccagaac ggcaccaatt tccggggtta atgcggcaaa
    12181 agaagaggca aaaagcagca acagtgtttt tgccagcaac cagctcagta ctgcgggtgc
    12241 caccagacgc atattcgccc aagtcagccg catactgcta cgcatcgagg caaaaatgcc
    12301 cattttgtcc tgtaccagca tcaccggtgc cagggccagt aaaatggcca tgataattcc
    12361 cggaacgacc accagcatga tgccaatctg tactaaaagg gtagtcagaa aaatcagaat
    12421 aaataacttg ggcaatatcg gcgcactggc accaatagct cgcagcgcac tgactctctg
    12481 acccgcagac accagctgga taattaatat tacgcctccg gcgagaatgg cgttaccgat
    12541 taatcctgaa aaagtggacg ccgctgaagc ctgcagcaaa atttgttgct gttccggtga
    12601 catattctga accaggtcga acaacccact actgccgcta acgggcacgc cgtcattgag
    12661 ctgcgcaagc tgtgcatcac tgggtgagaa aacatgccct aacaccactg tgataaacgc
    12721 acatagcaac gataccaaca gaatggtcat aaattgatta cggaagaaat ttccggtgtc
    12781 acggtatacg gactgcgccg tgatagacat gcactctcct tgagttttat gcaggtgtta
    12841 attagcgggc aattgtaccc tggttaagcc tcgggtggca gcatcaggtc ttgtatggaa
    12901 aagcatatct ttgtaaagcg gaggtaattg ctggccttca ggcgtgggca aaccgcggca
    12961 agtggcgatc gagttaatat ttgcgtagag aaaatattta aaaattgatt taaatcacat
    13021 taaccaggat tctcaatgca acttctaaat taatccagat caataaatgg tgaattatca
    13081 tatgtaatgt gatctatgta ggatcatttg ttactccaat gtaggtatat tcgtcacgtt
    13141 tttataacca taacgacgga gcggatatga aaaagttaac agtggcggct ttggcagtaa
    13201 caactcttct ttctggcagt gcctttgcgc atgaagcagg cgaatttttt atgcgtgcag
    13261 gttctgcaac cgtacgtcca acagaaggtg ctggtggtac gttaggaagt ctgggtggat
    13321 tcagcgtgac caataacact caactgggct tgacgtttac ttatatggcg accgacaaca
    13381 ttggtgtgga attactggca gcgacgccgt tccgccataa aatcggcacc cgggcgaccg
    13441 gcgatattgc aaccgttcat catctgccgc caacactgat ggcgcagtgg tattttggtg
    13501 atgccagcag caaattccgt ccttacgttg gggccggtat taactacacc accttctttg
    13561 ataatggatt taacgatcat ggcaaagagg cagggctttc cgatctcagt ctgaaagatt
    13621 cctggggagc tgccgggcag gtgggggttg attatctgat taaccgtgac tggttggtta
    13681 acatgtcagt ctggtacatg gatatcgata ccaccgccaa ttataagcta ggcggtgcac
    13741 agcaacacga tagcgtacgc ctcgatccgt gggtgtttat gttctcagca ggatatcgtt
    13801 tttaattcca caaaaaaacg accccgtaat atacggggtc aataaggaca tggcataaag
    13861 tgtattattt cttcgcttct acgccgtcag tttcagagcg aattaaaaat ttctcagttg
    13921 tttgcggaat atgctgaatc agccagtcgg ccatttgctt ttcctcattt aaaatcgctt
    13981 cgatggttgg aattgaagct gtatcaccgg cattttttgc tgctgctaat agtgaggtgt
    14041 aacaggcgat ttcaaattgc tcgaagacat atccgctaat agagcctttg actatttcat
    14101 cagaagggaa tataccaccg attgactgcc caagcgcagc cattttactc atggaatctt
    14161 taatgactga acgtgaaatg tcattacgat caagaatagt ttccaattga acaatctggt
    14221 ttttggtttc actaagatgt tgttcaatac gagcgcgtag ttcaggataa ttatctatac
    14281 ggctggccat ggattcaagc atagattcgg cttgcttttc cattgcgtgg gcgtcacgta
    14341 accagtcatg ataatgttca atacgattca tgaaaatgtc ctctttataa aatgactaaa
    14401 agttaaagtt catatttcag gctttatttt cggctttctt atttacgtta ttaagggcca
    14461 gatcagtcag tttgatgtcg gtggcctttt cttcttccag ggtctctttc agaagcttcg
    14521 ctgctttacg ataacctaat tgttcagcca gcgtcgctaa tgtcccgtaa ctggcaatct
    14581 cataatgctc gactttctgt gctgcggcaa tcagtgcggc atcacgcact tcgtttttct
    14641 cggtactttc gatgacctca ttagcttctt caataagacc ttccattgcc acacatttca
    14701 tgcgcttaat tttcagattc gattctgatt ccacaacttg atcaatacgt tcaatctgtc
    14761 catgtgtttc ctcgaggtgc gcatgaaaag cctgacttaa tttttcgttt gatgttgctc
    14821 ttgcgagttt tgccagtgcc cgggttaatt gtttttctgc gctgtaggta tctgaaagca
    14881 ggtgaataaa tacatcttca atggtcttca tattcatatt tttctccagt gaaatcactg
    14941 cgagccatac ggcccgcaga tgcaattatc tttatcagct gatattaata attaacggtg
    15001 aatcaggatt tgcctgattt attaccaccg ctttgttgac cgcctttttt acccgcttca
    15061 gatgcgcgtt gcggatcatt tttaaaatta ccgccgctat gctgaccgcc tttacggcct
    15121 gcgtcggatg ccttctcacg gtcttcggcg aaatttcctg aaccaccacg atgttcggcc
    15181 atgttatttc tcccgttgcg ttgcattgtt tcattaatat gagtgttgtg tgtcgacact
    15241 cattaaaatt agtcgctaat gagaattagt caaattaagc gcaacgagaa gatagaggga
    15301 aaatatattt tgaggaacat tctggatata ttaacaatta cctgaggaat aagtgactta
    15361 gagaa
//
LOCUS       DAPHDW010000104        15277 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.105, whole genome shotgun sequence.
ACCESSION   DAPHDW010000104 DAPHDW010000000
VERSION     DAPHDW010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15277
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.105"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..1804)
                     /locus_tag="RH057_003226"
                     /pseudo
     CDS             complement(1..1804)
                     /locus_tag="RH057_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
     gene            2328..4256
                     /gene="thrS"
                     /locus_tag="RH057_003227"
     CDS             2328..4256
                     /gene="thrS"
                     /locus_tag="RH057_003227"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HDV3129556.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEDGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            4260..4802
                     /gene="infC"
                     /locus_tag="RH057_003228"
     CDS             4260..4802
                     /gene="infC"
                     /locus_tag="RH057_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HDV3129557.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPGT
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            4899..5096
                     /gene="rpmI"
                     /locus_tag="RH057_003229"
     CDS             4899..5096
                     /gene="rpmI"
                     /locus_tag="RH057_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HDV3129558.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            5149..5505
                     /gene="rplT"
                     /locus_tag="RH057_003230"
     CDS             5149..5505
                     /gene="rplT"
                     /locus_tag="RH057_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HDV3129559.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTALVEKAKAALA"
     misc_feature    5622..5746
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            5628..5672
                     /gene="pheM"
                     /locus_tag="RH057_003231"
     CDS             5628..5672
                     /gene="pheM"
                     /locus_tag="RH057_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HDV3129560.1"
                     /translation="MNAAIFRFFFYFST"
     gene            5956..6939
                     /gene="pheS"
                     /locus_tag="RH057_003232"
     CDS             5956..6939
                     /gene="pheS"
                     /locus_tag="RH057_003232"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HDV3129561.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DTTRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVGIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            6954..9341
                     /gene="pheT"
                     /locus_tag="RH057_003233"
     CDS             6954..9341
                     /gene="pheT"
                     /locus_tag="RH057_003233"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HDV3129562.1"
                     /translation="MKFSELWLREWVNPAIDSDALANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPALQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLPSPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            9346..9645
                     /gene="ihfA"
                     /locus_tag="RH057_003234"
     CDS             9346..9645
                     /gene="ihfA"
                     /locus_tag="RH057_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HDV3129563.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            9746..10726
                     /gene="btuC"
                     /locus_tag="RH057_003235"
     CDS             9746..10726
                     /gene="btuC"
                     /locus_tag="RH057_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HDV3129564.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPLWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKARR"
     gene            10789..11340
                     /gene="btuE"
                     /locus_tag="RH057_003236"
     CDS             10789..11340
                     /gene="btuE"
                     /locus_tag="RH057_003236"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HDV3129565.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTAVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGLVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            11340..12089
                     /gene="btuD"
                     /locus_tag="RH057_003237"
     CDS             11340..12089
                     /gene="btuD"
                     /locus_tag="RH057_003237"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HDV3129566.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            12167..12631
                     /locus_tag="RH057_003238"
     CDS             12167..12631
                     /locus_tag="RH057_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3129567.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLQLPRETKEQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            12878..13591
                     /gene="ydiV"
                     /locus_tag="RH057_003239"
     CDS             12878..13591
                     /gene="ydiV"
                     /locus_tag="RH057_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HDV3129568.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLERDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLDKSFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTTEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            13654..15090
                     /locus_tag="RH057_003240"
     CDS             13654..15090
                     /locus_tag="RH057_003240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HDV3129569.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFGVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLIRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSYLEDDEDKYRLW
                     FSDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLHRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLRDEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMTELHRLHEAL
                     RNPFSDRADDYVSRPPDWGKRLEVSCSS"
     gene            complement(15094..>15277)
                     /gene="hemP"
                     /locus_tag="RH057_003241"
     CDS             complement(15094..>15277)
                     /gene="hemP"
                     /locus_tag="RH057_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HDV3129570.1"
                     /translation="TDSRKLTPETDANHKTASPQPIRRISSQTLLGPDGKLIIDHDGQ
                     EYLLRKTQAGKLLLTK"
BASE COUNT         3883 a         3469 c         4004 g         3921 t
ORIGIN      
        1 tatgatgaat ggctatatga acagctgaca tgttttcatg atttttagcg gccaacagtg
       61 tgaataattg acgttgacta aaagaatatt tttttgcaaa agtactcagt gttgatatat
      121 aagataacac gacgtcttta ttacccttgc tcatggcgat gtataaagca ggggttccat
      181 gtgctgtcgc gccttttaat aagtccatga tgttgatttt gctgagttta tatttaacgg
      241 cgatcccata aactttagag aggaaccgtg tgaaacacaa agggtgatta ttttccattg
      301 cagcaaataa ccctggttca ccatcacgtg ttttcatggc taaaatatcc atcaccattt
      361 ttttgtttaa aagttcttgt gctattggag tggttaatgc attcagaaag atatcgatta
      421 tttcatcgaa tccatacaac atggagatat agagtccagg tgtttcgtta gcacttttag
      481 tttgaagtaa tttaaccaga ttatcttcat gaatgatatg attgtatacc agtgactgga
      541 tttcttgcat aaatattttt acgttgtcag tttgtccgtt catcatagcc agaaataaag
      601 caggtgtacc aacgctattc tgcgcagtta ttatttcaat gatttctttt tcagggagta
      661 tgggcaactg tttgaatagt gtaataagct attgagtctg gcctgtggcc attattaaca
      721 acattgccga tggatgtata ggggcaaatg gcattcgtgt cgtgataccc gctaaaatag
      781 caactggaac tccctcaggt aatagaggaa gaatagcaat tgcacagttg cgaacatacc
      841 tatcagccca ggtctgagca tcgggacctg aactgatgaa cttatcaagc gagagaaagc
      901 cccgcgctgt cctgtgggtg cctctgacgg caacgttggt atcgttcggg tcgtaaacag
      961 acacaacata atttaattgt ccatacactg actgctttat tcgtagcctt actgacaaag
     1021 tatgattgtc tatatataaa atcgcggaca tatgactgac attgtttgcc atcattcgac
     1081 aaaagaaaat atctaaagcg aagccaaaat ccttactcgc gataacgcga ctccctgcag
     1141 gaggatggcg aagtaatgct ctgaactccg catcccggac taaggggata tgctgctgta
     1201 tggattcaac ggaggagtaa tgtttaatca ggttgacttt gccttttcca tttctcaatg
     1261 aatcaagggc gtattgcaat gacagatggc gacaaaaaat agcgtcagaa ggagattgca
     1321 aaaaacatgc actaccattg agatttactg ttgggtgtat agttttttct ctatggattt
     1381 ttttagacag tttcatacaa tggttacgat cttcagcagt taactgttcc tgttcaatat
     1441 aatccagtat gcttcgcaaa tggttagctt taataggcgt ggtttccagt aataaggaaa
     1501 tgaaatcttt tttatagtgt aatgtaatga gtatattttc tgcatggatg ggtgaagtgg
     1561 caaagttaat ggtaaaaagg gcagtggaat aacaattttg tgatattttt cgacagtttg
     1621 cggtaaattc ctgaataaca cagtatgaca gcatcgaact taagtcagag gtatcaagta
     1681 gggtgatgag ctggcgcgca aatttattaa taggctctga tgaaggtgtt ggtatgtcgc
     1741 tatcgatgtg caatctatga ggagacttat aatgagttct gataatgata tcgttttgac
     1801 tcataatcta ttcgccttat atatatctgg aggttttgag gatgatgatt aattattatc
     1861 gccagaacga aatgtgcacg aaaaattgtt ataagaattg tgtcactttt atcattgtga
     1921 tatgaccact tcgtcattaa tctcatgagc gtttaaaata ctaccgccaa atcacgcaac
     1981 gaactttgct ggattcagtt tatgctgctg taaatccgct cgcagtaaac ctttcagacg
     2041 caccgtgatg ttatcagtgg ttctttaagc gttttgctgg tgtactcact acaaacgaat
     2101 tgcgaatcga atcaatgtga aacggaaagg tacaatctcc ctttttttag tgttaacatc
     2161 gctcaaccgg gtggaaggtg agaggttaat gtctttcatt tgtgtacctg attgcgaacc
     2221 aatttagcat ttgttggcta gatggtttcg caatgaactg ttaataaaca aatttttctt
     2281 tgtatgtgat ctttcgtgtg ggtcaccact gcaaataagg atataaaatg cctgttataa
     2341 ctcttcctga tggcagccaa cgccattacg accacgctgt aagccccatg gatgttgcgc
     2401 tggacattgg tccaggtctg gcgaaagcct gtatcgcagg gcgcgttaat ggcgaactgg
     2461 ttgatgcttg cgatctgatt gaaaacgacg cacaactgtc catcattacc gccaaagacg
     2521 aagacggtct ggagatcatt cgtcactcct gtgcgcacct gttagggcac gcgattaaac
     2581 aactttggcc gcataccaaa atggcaatcg gcccggttat tgacaacggt ttttattacg
     2641 acgttgatct tgaccgcacg ttaacccagg aagatgtcga agcactcgag aagcggatgc
     2701 atgagcttgc tgagaaaaac tacgacgtca ttaagaagaa agtcagctgg cacgaagcgc
     2761 gtgaaacttt cgccaaccgt ggggagagct acaaagtctc cattcttgac gaaaacatcg
     2821 cccatgatga caagccaggt ctgtacttcc atgaagaata tgtcgatatg tgccgcggtc
     2881 cgcacgtacc gaacatgcgt ttctgccatc atttcaaact aatgaaaacg gcaggggctt
     2941 actggcgtgg cgacagcaac aacaaaatgt tgcaacgtat ttacggtacg gcgtgggcag
     3001 acaaaaaagc gcttaacgct tacctgcagc gcctggaaga agccgcgaaa cgcgaccacc
     3061 gtaaaatcgg taaacagctc gacctgtacc atatgcagga agaagcgccg ggtatggtat
     3121 tctggcacaa cgacggctgg accatcttcc gtgaactgga agtgtttgtt cgttctaaac
     3181 tgaaagagta ccagtatcag gaagttaaag gtccgttcat gatggaccgt gtcctgtggg
     3241 aaaaaaccgg tcactgggac aactacaaag atgcaatgtt caccacgtct tctgagaacc
     3301 gtgaatactg cattaagccg atgaactgcc cgggtcacgt acaaattttc aaccaggggc
     3361 tgaagtctta tcgcgatctg ccgctgcgta tggccgagtt tggtagctgc caccgtaatg
     3421 agccgtcagg ttcgctgcat ggcctgatgc gcgtgcgtgg atttacccag gatgacgcgc
     3481 atatcttctg tactgaagaa caaattcgcg atgaagttaa cggatgtatc cgtttagtct
     3541 atgatatgta cagcactttt ggcttcgaga agatcgtcgt caaactctcc actcgtcctg
     3601 aaaaacgtat tggcagcgac gaaatgtggg atcgtgctga ggcggacctg gcggttgcgc
     3661 tggaagaaaa caacatcccg tttgaatatc aactgggtga aggcgctttc tacggtccga
     3721 aaattgaatt taccctgtat gactgcctcg atcgtgcatg gcagtgcggt acagtacagc
     3781 tggacttctc cttgccgtct cgtctgagcg cctcctatgt gggcgaagac aacgagcgta
     3841 aggtaccggt aatgattcac cgcgcaattc ttgggtcgat ggaacgtttc atcggtatcc
     3901 tgaccgaaga attcgctggt ttcttcccga cctggcttgc gccggttcag gttgttatca
     3961 tgaatattac cgattcacag tctgaatacg ttaacgaatt gacgcaaaaa ctatcaaatg
     4021 cgggcattcg tgttaaagca gacttgagaa atgagaagat tggctttaaa atccgcgagc
     4081 acactttgcg tcgcgtccca tatatgctgg tctgtggtga taaagaggtg gaatcaggca
     4141 aagttgccgt tcgcacccgc cgtggtaaag acctgggaag catggacgta aatgaagtga
     4201 tcgagaagct gcaacaagag attcgcagcc gcagtcttaa acaattggag gaataaggta
     4261 ttaaaggcgg aaaacgagtt caaacggcgc gccctaaccg tatcaatggc gaaattcgcg
     4321 cccaggaagt tcgcttaaca ggtctggaag gcgagcagct tggtattgtg agtctgagag
     4381 aagctctgga gaaagcagaa gaagccggag tagacttagt cgagatcagc cctaacgccg
     4441 agccgccggt ttgtcgtata atggattacg gcaaattcct ctatgaaaag agcaagtctt
     4501 ctaaggaaca gaagaaaaag caaaaagtta tccaggttaa ggaaattaaa ttccgtcctg
     4561 gtacagatga aggcgactat caggtaaaac tccgcagcct gattcgcttt ctcgaagagg
     4621 gtgataaagc caaaatcacg ctgcgtttcc gcggtcgtga gatggcgcac cagcaaatcg
     4681 gtatggaagt gcttaatcgc gtgaaagacg atttgcaaga actggcagtg gtcgaatcct
     4741 tcccaacgaa gatcgaaggc cgccagatga tcatggtgct cgctcctaag aagaaacagt
     4801 aaggccttca agtagcactg tctgtggagc cttcgggctt cacaggcttt gttcgccttt
     4861 gtttcgttta ttaacaatgc gaagtggaag ttattaaaat gccaaaaatt aagaccgtac
     4921 gcggtgctgc taagcgcttc aaaaaaaccg gtaaaggtgg ttttaagcac aagcacgcta
     4981 acctgcgtca cattctgacc aaaaaagcga ccaaacgtaa acgtcacctg cgtccgaaag
     5041 ccatggtttc caaaggcgat ctgggcctgg taatcgcgtg cctgccgtac gcataagccg
     5101 ttaacgtttt taacttttta attagaatat agatacagga gagcacatat ggctcgcgta
     5161 aaacgtggtg ttattgcacg cgcacgtcac aagaaaattt tgaaacaagc taaaggctac
     5221 tacggtgcgc gttctcgcgt ataccgcgtt gccttccagg ctgttatcaa agctggtcag
     5281 tatgcttacc gtgaccgtcg tcaacgtaag cgtcagttcc gtcaactgtg gattgcgcgt
     5341 atcaacgcag cagcacgtca gaacggtatt tcttacagca aattcatcaa tggcctgaaa
     5401 aaagcctctg ttgaaatcga ccgtaagatc ctggctgata tcgcagtatt cgacaaagta
     5461 gcgttcaccg ctctggttga aaaagcgaaa gcagctctgg cataagccag ttgaaagagg
     5521 gagctagtct ccctcttttc gtttcaacgc catcaaaaca ttgactttta tcgccgtagc
     5581 cttttcaata aaggtctttt gaagagtacc aaaaggtaac gcaagcaatg aatgctgcta
     5641 ttttccgctt ctttttttac tttagcacct gaatccagga ggctagcgcg tgagaagaga
     5701 aacggaaaac agcgcctgaa agcctcccag tggaggcttt ttttgtatgc gcgttttgaa
     5761 aaataaaaag cctgtctcat caggtgtaat tgtcgcagcc agtctggaca cggccagcgc
     5821 ggagaaaccg gagcgtattt aagtacgtga gaatttcgag cactgcccgg gaccaaaatg
     5881 gcaagtaaaa tagcctgatg ggataggctc taagtccaac gaaccagtgt caccactgac
     5941 acaatgagga aaaccatgtc acatctcgca gaactggttg ccagtgcgaa ggcggccatt
     6001 agccaggcgt cagatgttgc cgcgttagat aatgtgcgcg tcgaatattt gggtaaaaaa
     6061 gggcacttaa cccttcagat gacgaccctg cgtgagctgc cgccagaaga gcgtccggca
     6121 gctggtgcgg ttatcaacga agcgaaagag caggttcagc aggcgctgaa tgcgcgtaaa
     6181 gcggaactgg aaagcgctgc actgaatgcg cgtctggcgg cggaaacgat tgatgtctcc
     6241 ctgccaggtc gtcgcattga aaacggcggt ctgcatccgg ttacccgtac catcgaccgt
     6301 atcgaaagtt tcttcggtga gcttggcttt accgtggcaa ccgggccgga aatcgaagac
     6361 gattatcata acttcgatgc tctgaacatt cctggtcacc acccggcgcg cgctgaccac
     6421 gacactttct ggtttgacac tacccgcctg ctgcgtaccc agacctctgg cgtacagatc
     6481 cgcaccatga aagcccagca gccaccgatt cgtatcatcg cgcctggccg cgtttatcgt
     6541 aacgactacg accagactca cacgccgatg ttccatcaga tggaaggtct gattgttgat
     6601 accaacatca gctttaccaa cctgaaaggc actctgcacg atttcctgcg taacttcttt
     6661 gaggaagatt tgcagattcg cttccgtcct tcctacttcc cgttcaccga gccttctgca
     6721 gaagttgacg tcatgggtaa aaacggtaaa tggctggaag tgctgggttg cgggatggtg
     6781 catccgaacg tgctgcgtaa cgttggcatc gacccggaag tttactctgg tttcgccttc
     6841 gggatgggga tggagcgtct gactatgttg cgttacggcg tcaccgacct gcgttcattc
     6901 ttcgaaaacg atctgcgttt cctcaaacag tttaaataag gcaggaatag attatgaaat
     6961 tcagtgaact gtggttacgc gaatgggtga acccggcgat tgatagcgat gcgctggcga
     7021 atcaaatcac tatggcgggc ctggaagttg acggtgtaga accggttgcc ggtagcttcc
     7081 acggcgtggt cgttggtgaa gtggttgagt gtgcgcagca tccgaacgct gacaaactgc
     7141 gtgtgacaaa agtgaatgtt ggcggcgatc gcctgctgga catcgtctgc ggtgcgccaa
     7201 actgccgtca gggcctgcgt gtggcggtag ctaccattgg tgctgttctg ccgggtgatt
     7261 tcaaaattaa agcggcgaaa ctgcgtggtg aaccgtctga agggatgctg tgctcctttt
     7321 ctgagctggg aatttctgac gatcatagcg gcattatcga gctgcctgcg gatgcgccga
     7381 ttggcaccga catccgtgaa tacctgaaac ttgatgacaa caccatcgaa atcagcgtga
     7441 cgccaaaccg tgccgactgt ttaggtatca ttggtgttgc gcgtgacgtt gccgtgctga
     7501 atcagctgcc gctggttgaa ccggaaatcg ttccggttgg tgcgaccatc gacgacacgc
     7561 tgccgattac agtcgaagcg ccggaagcct gcccgcgtta tcttggccgt gtggtaaaag
     7621 gcattaacgt taaagcgcca actccgctgt ggatgaaaga aaaactgcgt cgttgcggga
     7681 tccgttctat cgatgcagtt gttgacgtca ccaactatgt actgctcgaa ctgggccagc
     7741 cgatgcacgc tttcgataaa gatcgcattg aaggcggcat tgtggtgcgg atggcgaaag
     7801 agggcgaaac gttggtactg cttgatggca ctgaagcgaa gctgaatgct gacactctgg
     7861 tcatcgccga ccacaacaag gcgctggcga tgggcggcat ctttggtggc gaacactctg
     7921 gcgtgaatga cgaaacgcaa aacgtcttgc tggaatgcgc tttcttcagc ccgctgtcta
     7981 tcaccggtcg tgctcgtcgt catggcctgc atactgatgc gtctcaccgt tatgagcgtg
     8041 gcgttgatcc ggcactgcag cacaaagcga tggaacgtgc gacccgtctg ctgatcgaca
     8101 tctgcggtgg tgaggctggt ccggtaattg atatcaccaa cgaagcaacg ctgccgaagc
     8161 gtgcaaccat cactttacgt cgtagcaaac tggatcgcct gatcggccat catattgcgg
     8221 atgagcaggt aactgacatt ctgcgtcgtc tcggctgcga agtgaccgaa ggcaaagacg
     8281 agtggcaggc agttgcgccg agctggcgtt tcgatatgga gattgaagaa gatctggtcg
     8341 aagaagtcgc gcgtgtttac ggctacaaca acatcccgga tgagccggta caggcaagcc
     8401 tgattatggg tactcaccgt gaagctgacc tgtcgctcaa gcgcgtgaaa acgctgctca
     8461 acgacaaagg ctatcaggaa gtgatcacct acagcttcgt tgatccgaaa gtgcagcaga
     8521 tgatccatcc aggcgttgaa gccttactgc tgccaagccc gatctctgtt gaaatgtcag
     8581 caatgcgtct ttctctgtgg accggcctgc tggcaaccgt ggtgtacaac cagaaccgtc
     8641 agcagaaccg tgtgcgcatt ttcgaaagcg gtctgcgttt cgtaccagat actcaggcac
     8701 cgttgggcat tcgtcaggat ctgatgttag ccggtgtgat ttgcggtaac cgttacgaag
     8761 agcactggaa cctggcaaaa gagaccgttg atttctatga tttgaaaggc gatcttgaat
     8821 ccgttctcga cctgaccggt aaactgaatg aggttgagtt ccgtgcagaa gcgaatccgg
     8881 cactgcatcc ggggcaatcc gcagcgattt atctgaaagg tgaacgtatt ggttttgttg
     8941 gggttgttca tcctgaactg gaacgtaaac tggatcttaa cggtcgcact ctggtgttcg
     9001 aactggagtg gaacaagctc gcagaccgcg tggtgcctca ggcgcgcgag atttctcgct
     9061 tcccggcgaa ccgtcgtgac atcgcggtgg tggtcgcaga aaacgttccc gcagcggata
     9121 ttttatccga atgtaagaaa gttggcgtaa atcaggtagt tggcgtaaac ttatttgacg
     9181 tgtaccgcgg taagggtgtt gcggaggggt ataagagcct cgccataagc ctgatcctgc
     9241 aagataccag ccgtacactc gaagaagagg agattgccgc taccgtcgcc aaatgtgtag
     9301 aggcattaaa agagcgattc caggcatcat tgagggattg aacctatggc gcttacaaaa
     9361 gctgaaatgt cagaatatct gtttgataag cttgggctta gcaagcggga tgccaaagaa
     9421 ctggttgaac tgtttttcga agagatccgt cgcgctctgg aaaacggcga acaggtgaaa
     9481 ctctctggtt ttggtaactt cgatctgcgt gataagaatc aacgcccggg acgtaacccg
     9541 aaaacgggcg aggatattcc cattacagca cggcgcgtgg tgaccttcag acccgggcag
     9601 aagttaaaaa gccgggtcga aaacgcttcg cccaaagacg agtaatctga tctaactaaa
     9661 aaggccgctc tgcggccttt tttcttttca ctgtcgaaga gtcaccgtaa aatcaacgcc
     9721 atgacacttc agcagaacgg ataccatgct gacacttgcc cgccaacaac agcgacaaaa
     9781 tattcgctgg ttattatgcc tgtcagtttt gatgctgctg gcgcttctct taagcctttg
     9841 cgccggtgaa caatggatct cgccaggtga ctggtttact cctcgtggcg aactgttcgt
     9901 ctggcaaatt cgcctgccac gtacgctggc tgtattgctg gttggtgcgg cgctggctat
     9961 atccggcgct gtaatgcagg cgttgtttga aaatcctctg gcagaacctg gactacttgg
    10021 cgtctctaac ggcgcaggcg tggggcttat cgccgcggta ttgcttgggc aagggcaact
    10081 ccccaactgg gcgctagggc tgtgtgcgat tgctggcgcg cttatcatca ctttaatact
    10141 cttacgtttc gcccgtcgtc atctttcgac cagtcggtta ttgctggctg gcgttgcatt
    10201 agggattatc tgtagcgcac taatgacgtg ggctatctac ttttccacct ctgttgattt
    10261 acgtcagctg atgtactgga tgatgggcgg ttttggcggc gtagactggc ggcaaagctg
    10321 gctgatgctg gcattgatcc ccgtgttgtt gtggatctgt tgtcagtcca ggccgatgaa
    10381 tatgttagca cttggcgaga tctcggcgcg gcaactgggt ttacccctgt ggttctggcg
    10441 caatgtgctg gtggccgcga ccggctggat ggttggcgtc agtgtggcgc tggcgggtgc
    10501 tatcggcttt attggtctgg tgatcccaca tattcttcgg ttgtgtggtt taaccgatca
    10561 tcgcgtatta cttcccggct gcgcgctggc aggggcgagc gcattgctgc tggccgatat
    10621 tgtagcgcgc ctggcattag ctgccgcaga gctgcctatt ggcgtggtca ccgcaacgtt
    10681 gggtgcgccg gtgtttatct ggttattgtt aaaagcaaga cgttagccgc aaaaagacgg
    10741 tctatgatta aaagctaaga tttttactga ccacacccag gagaaacgat gcaagattcc
    10801 attctgacga ccgtagtgaa agatatcgac ggtgaagtga ccacgctgga gaagtacgct
    10861 ggtaatgtgc tgttgattgt caatgtcgcc tcaaagtgtg gcttaacgcc gcaatatgag
    10921 cagttggaga atattcagaa agcctgggcc gatcgaggtt ttgtggtgct gggattcccg
    10981 tgcaaccagt ttctggaaca agaaccgggc agcgatgaag agattaaaac ttactgtacc
    11041 accacatggg gggtgacgtt cccgatgttc agtaagattg aagttaatgg cgaaggacgc
    11101 catccgctgt atcaaaaatt gattgccgca gcgccgaccg cagtcgcgcc ggaagagagc
    11161 ggattctatg cccgtatggt cagcaaaggt cgtgcaccgc tgtaccctga tgatatttta
    11221 tggaattttg agaaattttt agttggcagg gacggattgg tcatccagcg tttttccccg
    11281 gatatgacgc cggaagaccc cattgtgatg gaaagcatta aactggcgtt ggcaaaataa
    11341 tgtctattgt gatgcagtta caggatgttg cggaatctac ccgcctgggg ccactttctg
    11401 gcgaggttcg ggctggggag atcctgcacc tggtggggcc gaatggcgcg ggtaagagta
    11461 ccttgcttgc acgaatggcc ggaatgacca gcggtaaggg aagcattcag ttcgcggggc
    11521 aacccctgga agcatggtcc gcaacaaaac tcgcgctgca tcgcgcctat ctttcacaac
    11581 agcagacgcc gccgtttgca atgccggtct ggcactacct gacactgcat cagcacgata
    11641 aaacgcgtac cgaactactg aatgatgtcg caggggcgct ggctcttgat gacaaactcg
    11701 gacgtagcac caatcaactt tccggcggtg aatggcaacg cgtacgtctt gctgcggtgg
    11761 tgctgcaaat cacaccacaa gccaatcccg caggtcaatt gctgcttctt gatgagccga
    11821 tgaacagtct tgatgttgcg caacaaagtg cgttagacaa aattctgagc gctctgtgtc
    11881 agcaaggact ggcgattgtg atgagcagtc acgatctcaa ccacacattg cgtcatgcgc
    11941 atcgggcgtg gttgctaaaa ggtggaaaaa tgctggccag tggacgcaga gaagaggtgc
    12001 tcacgccgcc aaatctggcg caggcctatg ggatgaattt tcgccgtctg gatatcgaag
    12061 gtcacagaat gctgatttcg accatctgat ttcattagta tcttgaaaat cgggtaatta
    12121 cgacgctaaa ttaatgcaag aataaaaaac agaggaaccg tacggaatgc gtttctgcct
    12181 tattttgatc acagcactgt ttctggccgg gtgtagccac cataaagcac cgccgccaaa
    12241 tgccagactt tctgattcga ttaccgttat tgccggtttg aacgaccagc tacaaagctg
    12301 gcatggcacg ccgtatcgtt atggtggcat gacgcggcgc ggtgtggact gttcgggatt
    12361 tgtggttgta acgatgcgcg atcgtttcga tttgcagctg ccccgagaaa ccaaagaaca
    12421 agcctctatc ggcacgcaaa ttgataaaga cgagttgctg cctggtgacc tggtcttttt
    12481 caaaacgggg tccggacaaa atggtttgca tgtaggtatt tacgatacca acaaccaatt
    12541 tatccacgcc tctaccagca agggagtgat gcgttcctca cttgataatg tctattggca
    12601 gaaaaatttc tggcaggcga gacgaatcta gtatgtcgaa aaatggacgg cgatgataca
    12661 tcgccgtcca tttactcctg aatacagtag tattttgcag acaaaaattc cgtaaatgga
    12721 ttattcgttt ataatcagga taatctaaaa aaaggacccc tgaaaaagtg gttaacatgg
    12781 aacaaatgaa agttactcct tctgtaattg ttatgtctta tggcctcatc ccttttagcc
    12841 ggatactgaa aaacatcctt cgagagggac ggttaccatg aagatttttt tggagaatct
    12901 ttatcattct gattgctatt tcctgccgat aagagataac cagcaactcc ttgtcggcgt
    12961 tgagttaatt acccatttct ccagtgaaga tggcacggtg cgtatcccga cgagtcgggt
    13021 tatagcgcaa cttaccgagg agcaacattg gcaattattt tcagagcagt tggaattact
    13081 aaaatcatgc caacattttt ttattcaaca taaactcttt gcctggctta atttaacgcc
    13141 acaagtggct acattattat tagagaggga taattacgca ggcgaactat taaaatatcc
    13201 gtttattgaa ctgttgatta atgagaatta cccgcatctc aatgaaggaa aagataatcg
    13261 cgatctttta tccttatcgc aaatgtatcc ccttgtgtta ggcaatttgg gagcaggtaa
    13321 tagcacaatg aaagcggttt ttgatggtct tttcacccgc gtcatgctgg ataaaagttt
    13381 tattcagcaa caaatcacgc atcgctcctt cgaaccgttt attcgtgcga tccaggcgca
    13441 aatttcccct tgttgcaatt gcatcatcgc ggggggcatt gatacgacag aaatattggc
    13501 acagattacc cccttcgatt tccatgctct ccagggatgc ctgtggcctg ccgttcctat
    13561 aaatcagata acgacgttgg ttcagcgata accctcctgt ttgctggtgt ttaagacgag
    13621 agtaaccgtc tacactatca aacaggagga tctatgaccc tgtcttttat tacccgctgg
    13681 cgcgatgaat tgccagaaac ctatacagca ctttccccta cgcctttaaa taatgcccgg
    13741 ctgatttggc ataataccga actcgctaac acgctgagta ttccatcgtc gctgtttaaa
    13801 aatgctgcag gtgtctgggg cggcgaaacc ctactgcctg gtatgtcacc actggcgcag
    13861 gtttacagtg gtcatcagtt cggcgtctgg gcgggccaac tgggtgatgg gcgcggcatt
    13921 ttacttggcg aacaactgct tgctgatggc actacaatgg actggcatct gaaaggtgct
    13981 ggcctgacgc cttattcgcg aatgggtgat ggacgggcgg ttttacgttc gacgatacga
    14041 gaaagtctcg ccagtgaggc gatgcattat ctgggcattc cgacgacccg cgcgttaagt
    14101 atcgtcacca gcgattcgcc agtgtatcgg gaaacggtgg agccaggcgc gatgctgata
    14161 cgtgtggcac caagtcatct gcgctttggt catttcgaac atttttacta tcgccgcgag
    14221 ccggaaaagg ttcgtcagtt agctgacttt gccattcgtc attactggtc atatcttgaa
    14281 gatgatgagg acaaataccg cctctggttt agcgatgttg tcgcacgtac cgcatcgtta
    14341 attgcccaat ggcagacggt cggctttgct catggggtga tgaataccga taacatgtcg
    14401 ctgctggggc tgacgcttga ttacgggccg tttggttttc tggatgatta cgaacccggt
    14461 tttatttgta atcactcgga tcatcaaggg cgttatagct ttgataatca acctgctgtc
    14521 gcgttgtgga atttacatcg tctggcgcag acattgtcac catttgttgc cgtagatgcc
    14581 ctgaatgagg ccctggacag ctatcagcag gttttgttga cgcattatgg acaacggatg
    14641 cggcagaaac tgggcttcat gacggagcaa aaagaggata acgcgctact gaatgaatta
    14701 ttcagtctga tggcgcgaga gcgcagcgat tatacccgca cattccgcat gctgagtctg
    14761 accgagcagc acagcgcggc gtcaccgcta cgtgatgagt ttattgatcg tgcggcattt
    14821 gatgactggt ttgcccgtta tcgggggcgt ttgcaacaag acgaggttag cgatagtgag
    14881 cgtcagcagc tgatgcaaag cgttaaccca gctctggtgt tgcgcaactg gttggcgcaa
    14941 cgggcgattg aagcggcaga aaagggtgat atgacggaat tgcaccgcct gcatgaggcg
    15001 ttgcgaaatc ctttcagcga cagagctgat gactatgtca gtcgtccacc tgactggggt
    15061 aaacggctgg aagtcagctg ctcgagttaa aggctacttg gtcaacagca gcttgccagc
    15121 ctgagtttta cggagcagat attcttgccc gtcatgatcg ataatcagtt tgccatccgg
    15181 acctaacagt gtctggctgg aaattcgccg gatgggctgc ggggaagcgg tcttgtgatt
    15241 ggcatccgtt tcaggcgtga gttttctgct atccgta
//
LOCUS       DAPHDW010000105        15172 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.106, whole genome shotgun sequence.
ACCESSION   DAPHDW010000105 DAPHDW010000000
VERSION     DAPHDW010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15172
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.106"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(39..2024)
                     /gene="yjcS"
                     /locus_tag="RH057_003242"
     CDS             complement(39..2024)
                     /gene="yjcS"
                     /locus_tag="RH057_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl sulfatase YjcS"
                     /protein_id="HDV3129571.1"
                     /translation="MNNSRLFRLSRIVIALTAASGMMVNTAYATDEAKAATQYTQQVN
                     QNYAKSLPFSDRQDFDDAQRGFIAPLLDEGILRDANGKPYYRGEDYKFDINAPAPETV
                     NPSLWRQSQLNGISGLFKVTDRMYQVRGQDISNITFIEGDTGIIVIDPLVTPPSAKAA
                     LDLYFQNRPQKPIVAVIYTHSHTDHYGGVKGIISEADVKSGKVQVIAPAGFMDEAISE
                     NVLAGNIMSRRALYSYGLLLAHNPQGNIGNGLGVTLASGYPSIIAPNKTITKTGEKMI
                     IDGLEFDFLMTPGSEAPAEMHFYIPALKALCTAENATHTLHNFYTLRGAKTRDTSKWT
                     EYLNETLDMWGNDAEVLFMPHTWPVWGNKHINDYIGKYRDTIKYIHDQTLHLANQGYT
                     MNEIGDMIKLPPALANNWASRGYYGSVSHNARAVYNFYLGYYDGNPANLHPYGQVEMG
                     KRYVQALGGSARVINLAQEANKQGDYRWSAELLKQVIAANPGDQVAKNLQANNFEQLG
                     YQAESATWRGFYLTGAKELREGVHKFSHGTTGSPDTIRGMSVEMLFDFMSVRLDSAKA
                     AGKNISLNFNMSNGDNLNLTLNDSVLNYRKTLQSQADASFYISREDLHAVLTGQAKMA
                     DLVKAKKAKIIGNGAKLEEIIACLDNFDLWVNIVTPN"
     gene            2277..2594
                     /locus_tag="RH057_003243"
     CDS             2277..2594
                     /locus_tag="RH057_003243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001183281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HDV3129572.1"
                     /translation="MQPVFLFKKRTGAMGGHVAGTAGFCVAVPAFYSWSMSEVIDKDT
                     RSSERTDYPHYASEVALAFGSRVVGLELAKMIVDAWLGAQYEGGRHQQRVEAITAIEQ
                     RRN"
     gene            2663..2992
                     /gene="yjdP"
                     /locus_tag="RH057_003244"
     CDS             2663..2992
                     /gene="yjdP"
                     /locus_tag="RH057_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YjdP"
                     /protein_id="HDV3129573.1"
                     /translation="MKRFPLFLLFTLLTLSTVPAQADIIDDTIGNIQQAINDAYNPDR
                     GRDYEDSRDDGWQREVSDDRCRQYDDRRRQFEDRRRQLDDRQRQLDQERRQLEDEERR
                     MEDEYGR"
     gene            complement(3139..3897)
                     /gene="phnP"
                     /locus_tag="RH057_003245"
     CDS             complement(3139..3897)
                     /gene="phnP"
                     /locus_tag="RH057_003245"
                     /EC_number="3.1.4.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism protein PhnP"
                     /protein_id="HDV3129574.1"
                     /translation="MSLTLTLTGTGGAQGVPAWGCECAACARARRSPQYRRQPCSGVV
                     KFNDAITLIDAGRHDLTDRWSPGSFQQFLLTHYHMDHVQGLFPLRWGVGDVIPVYGPP
                     DEQGCDDLFKHPGLLDFSHTVEPFVVFDLQGLQVTPLPLNHSKLTFGYLLETAHSRVA
                     WLSDTAGLPEKTLKFLLNNHPQVMVIDCSHPPRADAPRNHYDLNTVLALNQVIRSPQV
                     ILTHISHQFDAWLMENALPSGFEVGFDGMEIGVA"
     gene            complement(3899..4333)
                     /gene="phnO"
                     /locus_tag="RH057_003246"
     CDS             complement(3899..4333)
                     /gene="phnO"
                     /locus_tag="RH057_003246"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoalkylphosphonate N-acetyltransferase"
                     /protein_id="HDV3129575.1"
                     /translation="MPACELRPATQYDTDAVYALICELKQAEFDHHAFRVGFNANLRD
                     PNMRYHLALLDGEVVGMIGLHLQFHLHHVNWIGEIQELVVMPQARGLNVGSKLLAWAE
                     EEARQAGAEMTELSTNVKRHNAHRFYLREGYEQSHFRFTKAL"
     gene            complement(4320..4877)
                     /gene="phnN"
                     /locus_tag="RH057_003247"
     CDS             complement(4320..4877)
                     /gene="phnN"
                     /locus_tag="RH057_003247"
                     /EC_number="2.7.4.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 1,5-bisphosphokinase"
                     /protein_id="HDV3129576.1"
                     /translation="MMGKLIWLMGPSGSGKDSLLAELRLREQTQLLVAHRYITRDASA
                     GSENHIALSEREFFTRAGQNLLALSWHANGLYYGVGIEIDLWLHAGFDVVVNGSRAHL
                     PQARARYQSALLPVCLQVSPEILRQRLENRGRENASEINARLARAARYTPQDCHTLNN
                     DGSLRQSVDTLLTLIHQKEKHHACL"
     gene            complement(4877..6013)
                     /gene="phnM"
                     /locus_tag="RH057_003248"
     CDS             complement(4877..6013)
                     /gene="phnM"
                     /locus_tag="RH057_003248"
                     /EC_number="3.6.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate diphosphatase"
                     /protein_id="HDV3129577.1"
                     /translation="MIINNVKLVLENEVVHGSLEVQDGEIRAFAESQSRLPEAMDGEG
                     GWLLPGLIELHTDNLDKFFTPRPKVDWPAHSAMSSHDALMVASGITTVLDAVAIGDVR
                     DGGDRLENLEKMINTIEETQKRGVNRAEHRLHLRCELPHHTTLPLFEKLVQREPVTLV
                     SLMDHSPGQRQFANREKYREYYQGKYSLTDAQMQQYEEEQLALAARWSQPNRESIAAL
                     CRARQIALASHDDATHAHVAESHQLGSVIAEFPTTFEAAEASRKHGMNVLMGAPNIVR
                     GGSHSGNVAASELAQLGLLDILSSDYYPASLLDAAFRVADDESNRFTLPQAVRLVTKN
                     PAQALNLQDRGVIGEGKRADLVLAHRQGNHIHIDHVWRQGKRVF"
     gene            complement(6010..6690)
                     /gene="phnL"
                     /locus_tag="RH057_003249"
     CDS             complement(6010..6690)
                     /gene="phnL"
                     /locus_tag="RH057_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnL"
                     /protein_id="HDV3129578.1"
                     /translation="MINVQNVSKTFILHQQNGVRLPVLNRASLTVNAGECVVLHGHSG
                     SGKSTLLRSLYANYLPDEGQIQIKHGDEWVDLVTAPARKVVEIRKTTVGWVSQFLRVI
                     PRISALEVVMQPLLDTGVPREACAAKAARLLTRLNVPERLWHLAPSTFSGGEQQRVNI
                     ARGFIVDYPILLLDEPTASLDAKNSAAVVELIREAKTRGAAIVGIFHDEAVRNDVADR
                     LHPMGASA"
     gene            complement(6801..7559)
                     /gene="phnK"
                     /locus_tag="RH057_003250"
     CDS             complement(6801..7559)
                     /gene="phnK"
                     /locus_tag="RH057_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnK"
                     /protein_id="HDV3129579.1"
                     /translation="MNKPLLSVNNLTHLYAPGKGFSDVSFDLWPGEVLGIVGESGSGK
                     TTLLKSISARLTPQQGEIHYENRSLYAMSEADRRRLLRTEWGVVHQHPLDGLRRQVSA
                     GGNIGERLMATGARHYGDIRATAQKWLEEVEIPANRIDDLPTTFSGGMQQRLQIARNL
                     VTHPKLVFMDEPTGGLDVSVQARLLDLLRGLVVELNLAVVIVTHDLGVARLLADRLLV
                     MKQGQVVESGLTDRVLDDPHHPYTQLLVSSVLQN"
     gene            complement(7556..8401)
                     /gene="phnJ"
                     /locus_tag="RH057_003251"
     CDS             complement(7556..8401)
                     /gene="phnJ"
                     /locus_tag="RH057_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate 5-phosphate
                     C-P-lyase PhnJ"
                     /protein_id="HDV3129580.1"
                     /translation="MANLSGYNFAYLDEQTKRMIRRAILKAVAIPGYQVPFGGREMPM
                     PYGWGTGGIQLTASVIGESDVLKVIDQGADDTTNAVSIRNFFKRVTGVNTTERTDDAT
                     LIQTRHRIPETPLTEDQIVIFQVPIPEPLRFIEPRETETRTMHALEEYGVMQVKLYED
                     IARFGHIATTYAYPVKVNGRYVMDPSPIPKFDNPKMDMMPALQLFGAGREKRIYAVPP
                     FTRVESLDFDDHPFTVQQWDEPCAICGSTHSYLDEVVLDDAGNRMFVCSDTDYCRQQS
                     EAKSQ"
     gene            complement(8394..9458)
                     /gene="phnI"
                     /locus_tag="RH057_003252"
     CDS             complement(8394..9458)
                     /gene="phnI"
                     /locus_tag="RH057_003252"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-ribose 1-methylphosphonate
                     5-triphosphate synthase subunit PhnI"
                     /protein_id="HDV3129581.1"
                     /translation="MYVAVKGGEKAIDAAHALQESRRRGDTDLPELSVAQIEQQLNLA
                     VDRVMTEGGIADRELAALALKQASGDNVEAIFLLRAYRTTLAKLAVSEPLDTTEMRLE
                     RRISAVYKDIPGGQLLGPTYDYTHRLLDFTLLANGEAPTLTTAHSEQQPSPHVFSLLA
                     RQGLAKFEEDSGAQPDDITRTPPVYPCSRSSRLQQLMRGDEGYLLALAYSTQRGYGRN
                     HPFAGEIRSGYIDVSIVPEELGFAVNVGELLMTECEMVNGFIDPPGERPHFTRGYGLV
                     FGMSERKAMAMALVDRALQAPEYGEHATGPAQDEEFVLAHADNVEAAGFVSHLKLPHY
                     VDFQAELELLKRLQQEQNHG"
     gene            complement(9458..10042)
                     /gene="phnH"
                     /locus_tag="RH057_003253"
     CDS             complement(9458..10042)
                     /gene="phnH"
                     /locus_tag="RH057_003253"
                     /EC_number="2.7.8.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnH"
                     /protein_id="HDV3129582.1"
                     /translation="MTLETAFILPVQDAQHSFRRLLKAMSEPGVIVALHQLKRGWQPL
                     NIATTSVLLTLADNDTPVWLSAPLSNDIVSQSLRFHTNAPLVSQPEQATFAVTDEAIS
                     SEQLNALSTGTAVAPEAGATLILQVASLSGGRMLRLTGAGIAEERMIAPQLPECILYE
                     LTERPHPFPLGIDLILTCGERLLAIPRTTHVEVC"
     gene            complement(10039..10491)
                     /gene="phnG"
                     /locus_tag="RH057_003254"
     CDS             complement(10039..10491)
                     /gene="phnG"
                     /locus_tag="RH057_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate C-P lyase system protein PhnG"
                     /protein_id="HDV3129583.1"
                     /translation="MHADTATRQHWMSVLAHSQPAELAARLKALNITADYEVIRAAET
                     GLVQIQARMGGTGERFFAGDATLTRAAVRLTDGTLGYSWVLGRDKQHAERCALIDALM
                     QQSRYFQNLSETLIAPLDADRMARIAARQAEVNASRVDFFTMVRGDNA"
     gene            complement(10492..11217)
                     /gene="phnF"
                     /locus_tag="RH057_003255"
     CDS             complement(10492..11217)
                     /gene="phnF"
                     /locus_tag="RH057_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate metabolism transcriptional regulator
                     PhnF"
                     /protein_id="HDV3129584.1"
                     /translation="MHLSTHPTSYPTRYQEIAAKLEQELRQHYRCGDYLPAEQQLAAR
                     FEVNRHTLRRAIDQLVEKGWVQRRQGVGVLVLMRPFDYPLNAQARFSQNLLDQGSHPT
                     SEKLLSVLRPASGHVADALGITEGENVIHLRTLRRVNGVALCLIDHYFADLTLWPTLQ
                     RFDSGSLHDFLREQTGIALRRSQTRISARRAQAKECQRLEIPNMSPLLCVRTLNHRDG
                     ESSPAEYSVSLTRADMIEFTMEH"
     gene            complement(11238..12017)
                     /gene="phnE"
                     /locus_tag="RH057_003256"
     CDS             complement(11238..12017)
                     /gene="phnE"
                     /locus_tag="RH057_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HDV3129585.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLAVLVVSWQGAEMAPLTLIKDG
                     GNMATFAADFFPPDFSQWQDYLTEMAVTLQIAVWGTALAVVLSIPFGLMSAENLVPWW
                     VYQPVRRLMDACRAINEMVFAMLFVVAVGLGPFAGVLALFIHTTGVLSKLLSEAVEAI
                     EPGPVEGIRATGANKLEEILYGVLPQVMPLLISYSLYRFESNVRSATVVGMVGAGGIG
                     VTLWEAIRGFQFQQTCALMVLIIVTVSLLDFLSQRLRKHFI"
     gene            complement(12123..13139)
                     /gene="phnD"
                     /locus_tag="RH057_003257"
     CDS             complement(12123..13139)
                     /gene="phnD"
                     /locus_tag="RH057_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3129586.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLSPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLIPGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEMQGVKSNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            complement(13164..13952)
                     /gene="phnC"
                     /locus_tag="RH057_003258"
     CDS             complement(13164..13952)
                     /gene="phnC"
                     /locus_tag="RH057_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HDV3129587.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHGEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQQFDNERFDHLYRSINRIEENAKAA"
     gene            complement(14085..14528)
                     /gene="yjdN"
                     /locus_tag="RH057_003259"
     CDS             complement(14085..14528)
                     /gene="yjdN"
                     /locus_tag="RH057_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HDV3129588.1"
                     /translation="MPLSPYLSFAGNCADAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAMPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            complement(14688..15023)
                     /locus_tag="RH057_003260"
     CDS             complement(14688..15023)
                     /locus_tag="RH057_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HDV3129589.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
BASE COUNT         3067 a         4180 c         4507 g         3418 t
ORIGIN      
        1 gcttttgggg gcttcagcgc ccccaaaagc gagtgacatt aatttggggt tacgatattc
       61 acccacaaat cgaaattatc cagacaggcg ataatttctt ccagtttcgc gccattgcca
      121 ataattttgg ctttcttcgc ttttaccaga tccgccattt tggcttgccc ggtcagcacg
      181 gcgtgcagat cttcacggct gatgtagaaa gaggcgtcag cttgggattg cagtgtttta
      241 cggtagttaa gcacgctatc gttcagcgtc aggttgaggt tatcgccatt gctcatattg
      301 aagttcaggc tgatattttt acccgcggct ttcgcgctat cgaggcgaac ggacataaag
      361 tcgaacagca tttcgaccga catcccgcga atggtgtccg gggaaccggt ggtgccgtgg
      421 ctgaacttat gcaccccttc gcgcagctct ttcgcgccgg tcaggtagaa accgcgccag
      481 gtggcggact cggcctgata gcccagctgt tcaaagttat tcgcttgcag attcttcgcg
      541 acctggtcac ccgggttggc ggcaatcacc tgtttcagca gttctgccga ccagcggtaa
      601 tcaccttgct tgttcgcttc ttgcgccagg ttgatgacac gggcagaacc gcccagcgcc
      661 tgcacgtaac gtttacccat ctccacctga ccatacggat gcaggttagc cgggttaccg
      721 tcgtaatagc caagatagaa gttatacacc gcgcgggcgt tgtggctgac agaaccgtaa
      781 tagccacggc tggcccagtt attggcaagt gcaggcggca gtttaatcat gtcgccgatt
      841 tcattcatgg tgtagccctg gttcgccagg tgcagggtct ggtcgtgaat gtacttgata
      901 gtatcgcgat atttaccaat ataatcattg atatgcttat tgccccagac cggccaggtg
      961 tgcggcataa acaggacttc cgcgtcgtta ccccacatat ccagcgtttc gttcagatac
     1021 tcggtccact tgctggtgtc gcgggttttc gcgccgcgca gagtgtagaa gttgtgcagg
     1081 gtatgcgtgg cgttctcggc ggtacacagc gctttcaggg ccggaatata gaagtgcatt
     1141 tcggctggtg cttcgctacc tggggtcatc aggaagtcaa actccaggcc gtcgataatc
     1201 atcttctcac cggttttggt gatggttttg ttcggtgcga tgatgctcgg gtagcccgat
     1261 gccagcgtta cgccaagacc attgccgatg ttaccctgag ggttatgcgc cagcagcagg
     1321 ccgtaggagt aaagtgcacg gcggctcata atattacccg ccagtacgtt ttcgctgatg
     1381 gcttcgtcca taaagccagc aggggcgata acctgcactt taccggattt cacatcggct
     1441 tcagagataa tgcctttcac gccgccatag tggtcggtat ggctgtgggt ataaataacc
     1501 gcaacaatcg gtttttgcgg gcgattttgg aagtaaaggt caagggcggc ttttgcgctc
     1561 ggtggcgtca ccagcgggtc gatgacaata atcccggtat caccttcgat aaaggtgatg
     1621 ttggagatat cctgaccgcg aacctggtac attctgtcgg tgactttaaa caggccggaa
     1681 ataccgttta actgcgactg acgccacagg ctagggttaa cggtttcggg cgcgggcgca
     1741 ttgatatcaa atttataatc ttccccgcga taatatggtt tgccattagc atcgcgcaga
     1801 ataccttcat ccagcagcgg agcgataaat ccacgctgag cgtcgtcaaa atcctgacga
     1861 tcgctaaacg gtaatgattt ggcgtaattc tgattaacct gttgggtata ttgagtggcg
     1921 gctttcgctt catctgttgc gtaagcggta tttaccatca tgccgctggc agcagttaac
     1981 gcaataacaa tcctgctcaa acggaataac cgagagttat tcatataaat ctccatttat
     2041 catttgtgtc gtaaatatgt aatccatgca aaatacgtca tttaaagctg gcgttatcat
     2101 aattgggaaa ttgcgacttt aataagtgga agtgtgagcg gaacgagcca ttttattagg
     2161 catttgtgct gaagagggaa aattacgcga atttattatc tctgtaaata atatgaggtg
     2221 ttttatatat ttattatata tattgtactt cggcaaatac tgagcacaat ttatcaatgc
     2281 aaccggtgtt tctttttaaa aaacgtaccg gagcaatggg cggtcatgtc gctggcaccg
     2341 caggattctg cgttgctgtg ccagcctttt attcatggtc aatgagtgag gtgattgata
     2401 aagacaccag gtcgtcagag cgcactgatt atccgcatta tgccagtgaa gttgcactgg
     2461 cttttggttc acgagtggtt ggcctcgaac tggcaaaaat gattgtggat gcgtggctgg
     2521 gcgcacagta cgaaggcggt cgtcatcaac aacgcgtgga ggcgattacg gcaatagagc
     2581 agcggagaaa ttgagattca tccactactt gcatggatga gtaatgatta atgtggatag
     2641 agtttctttt tgaggttggc taatgaaacg ctttccgcta tttcttttat tcaccctgct
     2701 cacgctgtcc accgttccgg cccaggctga tattatcgac gatactattg gtaatattca
     2761 gcaggcgatt aatgacgctt ataaccctga ccgtgggcga gactatgaag attcgcgcga
     2821 cgacggctgg cagcgtgaag tgagcgacga tcggtgcaga caatatgacg accgccgccg
     2881 ccagtttgaa gatcgccgca ggcagctgga cgatcgccag cgccagcttg atcaggagcg
     2941 tcggcaactg gaggatgaag agcggagaat ggaagatgag tatgggcgat gaggttgggt
     3001 atggagcagg catcgcccct caccccaatg gggagaggga gaaaacgagc gcaatattca
     3061 atatcgagca caatcggtcc cctcgcccct ctggggagag ggttagggtg agggggccag
     3121 cgcgcacatt ccctctcatc acgccacccc aatctccatc ccatcaaacc ccacctcaaa
     3181 ccctgacggt agtgcgtttt ccatcagcca cgcatcaaac tggtggctga tatgggtcag
     3241 aatcacctgt ggcgagcgga taacctgatt cagcgcaagc acggtattta aatcatagtg
     3301 attacgcggt gcatccgcgc gcggcgggtg actgcaatca atcaccatca cctgcggatg
     3361 attattgagt aaaaatttca gcgttttttc cggcaggcca gcggtgtcag acagccacgc
     3421 cacccggcta tgtgccgttt ccagcagata accgaaggtc agttttgagt ggttaagcgg
     3481 cagcggcgtg acctgcaacc cctgcaaatc aaacaccaca aacggttcca ctgtgtggct
     3541 gaaatcaagc aggcccggat gtttaaacag atcgtcgcag ccctgttcat ccggcgggcc
     3601 gtagaccggg atcacatcgc ccacgcccca gcgtagcgga aatagcccct ggacgtgatc
     3661 catatggtag tgcgtcagca aaaactgctg gaacgatcca ggcgaccagc gatcggtgag
     3721 atcgtgccgc ccggcgtcga tcagggtgat cgcgtcgtta aacttcacta cgccgctgca
     3781 tggctggcgg cgatactgcg gcgagcgccg cgctctggcg caggccgcgc actcgcagcc
     3841 ccatgccgga acgccctgtg cgccgccagt gccggtgagc gtgagggtca ggctcatgtt
     3901 acagcgcctt ggtgaagcgg aagtggctct gctcgtagcc ttcacgcagg taaaaacggt
     3961 gtgcgttgtg gcgcttcacg ttggtggaaa gctcggtcat ttcggccccg gcctggcggg
     4021 cttcttcttc tgcccatgcc agtaatttac tgccgacgtt cagaccgcgc gcctgcggca
     4081 tcaccaccag ttcttgaatt tcgccgatcc agttgacatg atgcagatga aactgcaaat
     4141 gcaggccgat catgccgaca acttcgccat ccagcagcgc cagatggtag cgcatgtttg
     4201 ggtcgcgcag gttggcgtta aagcccacgc gaaacgcatg gtggtcaaac tccgcctgtt
     4261 ttagctcaca aatcagcgcg taaaccgcgt cggtatcgta ctgcgtggcc gggcgaagct
     4321 cacaagcagg catggtgttt ctccttctga tggatcagcg tcagcagcgt gtcgaccgac
     4381 tggcgcaggc tgccgtcatt gttgagcgta tggcaatcct gtggagtata gcgggcggcg
     4441 cgcgccaggc gggcgttaat ttcactggcg ttttcacggc cacggttttc caggcgctgg
     4501 cgcaggatct ccggcgaaac ctgtaaacag acgggcagca gcgccgattg atagcgcgcc
     4561 cgtgcctgcg gcaaatgcgc gcgtgaaccg ttgacgacta cgtcgaatcc agcgtgcagc
     4621 cagaggtcaa tctcgatgcc gacgccataa tacagaccgt tagcgtgcca gcttaaggcc
     4681 agcaggtttt gccccgcgcg ggtaaaaaac tcccgctcac tcagggcgat atggttttca
     4741 cttccggcgc tggcgtcgcg cgtgatgtag cgatgcgcca ccagtaactg cgtttgttcc
     4801 cgcaggcgga gttccgccag caggctgtct ttcccggagc cggacggccc cattaaccaa
     4861 atcagttttc ccatcatcag aacacccttt taccctgacg ccagacgtgg tcgatatgaa
     4921 tgtgattgcc ctggcgatgc gccagcacca ggtcagcgcg tttaccctcg ccaatcaccc
     4981 cgcgatcctg aagattcagc gcctgcgccg gatttttggt caccagcctc accgcctgcg
     5041 gtagcgtaaa gcggttgctc tcgtcatcgg cgacgcggaa cgccgcatcc agcaggctgg
     5101 cggggtagta gtcggaagag aggatatcca gcagaccaag ctgcgccagt tcactggccg
     5161 ccacattgcc ggagtgcgag ccgccacgca cgatatttgg cgcacccatc agcacgttca
     5221 tgccatgctt acgcgaggct tccgccgctt cgaacgtggt gggaaattcg gcgatcacgc
     5281 tgccaagttg gtgggattcg gcaacgtggg cgtgtgtggc gtcatcgtgg ctggcgagcg
     5341 caatttgtcg cgcgcggcac agggcggcga tggattcgcg attcggctgc gaccagcgtg
     5401 cggcgagcgc caattgctct tcttcgtact gctgcatctg cgcatcggtg agggagtatt
     5461 tgccctgata atattcgcga tacttctcgc ggttagcgaa ctggcgctgg cccggtgagt
     5521 ggtccatcag cgacaccagc gtcaccggtt cgcgctgaac cagtttttca aacagcggca
     5581 gtgtggtgtg atgcggcagt tcgcagcgca ggtgcaggcg gtgctcggcg cggttgacgc
     5641 cgcgtttctg cgtctcttcg atggtgttga tcatcttctc cagattctcc agccgatcgc
     5701 cgccgtcgcg cacgtcgcca atcgccaccg cgtccagcac ggtggtgatg ccgcttgcca
     5761 ccatcagcgc gtcgtggctg ctcatcgccg aatgggcggg ccagtcgact ttcgggcgcg
     5821 gggtgaagaa tttatcgagg ttgtcggtat gcagctcaat caggccgggt agtagccagc
     5881 caccttcgcc gtccatcgct tctggcaggc ggctctggct ttcggcaaag gcgcggattt
     5941 cgccatcctg cacctccagc gaaccgtgta ccacttcgtt ttccagcacc agtttgacgt
     6001 tattgataat catgcagagg ctcccattgg gtgcaagcgg tcggcgacgt cattacgtac
     6061 ggcttcgtca tggaagatgc cgacgatggc cgcgccacgg gttttggctt cgcgaatcag
     6121 ctcgactacg gcggcgctgt ttttggcgtc cagcgaggcg gtaggttcgt cgagcagcag
     6181 aatggggtag tcgacgataa agccgcgggc gatgttgacg cgctgctgtt cgccaccgga
     6241 aaaggttgat ggcgccaggt gccacaggcg ttccggcacg ttcaggcggg tgagcagacg
     6301 cgcggcttta gcggcgcagg cttcacgcgg aacgccggta tcgagcagcg gctgcatcac
     6361 cacttccagt gctgaaatac gcgggatgac gcgcagaaac tggctcaccc agccgacggt
     6421 ggttttgcgg atctccacca ctttgcgggc tggcgcggtg accaggtcta cccactcgtc
     6481 accgtgtttg atctggattt gaccttcatc gggcagatag ttggcgtaca gcgagcgcag
     6541 cagggttgat ttgccgctgc cggagtgacc gtggagcacc acgcattcgc ccgcgttgac
     6601 ggtgagcgag gcgcggttga ggacgggcag gcgcacgccg ttttgctggt gcaggatgaa
     6661 ggttttactg acgttttgta cgttaatcat ttttcgcctc gtggttcggg tgtcggatgc
     6721 gacgctgacg cgtcttatcc gacctacggg gagcgcattt gtaggccgga taaggcgttt
     6781 acgccgcatc cggcaccggc tcaattctgc aaaaccgatg acaccagcag ctgtgtatac
     6841 ggatgatgcg ggtcgtcgag cacgcggtcg gttaacccac tctccaccac ttgcccctgc
     6901 ttcatcacca gcaaacggtc cgccagcagg cgggcaacgc ctaaatcatg ggtgacaatc
     6961 accaccgcca ggttcagctc caccaccagg ccgcgcagca ggtcgagcag gcgggcctgc
     7021 accgacacat ccagcccgcc ggtcggttca tccataaaca ccagcttcgg atgcgtcacc
     7081 aggttgcggg caatctgcaa acgttgctgc atgccgccgg aaaaggtggt cggcaggtcg
     7141 tcgatccggt tggcgggaat ttccacctct tccagccact tctgcgcggt ggcgcgaatg
     7201 tcgccgtaat gacgcgcccc ggtcgccatc agccgctcgc cgatattgcc gcctgccgag
     7261 acctgacggc gcaggccgtc gagtggatgc tgatgcacta cgccccattc ggtgcgcagc
     7321 aggcgacggc ggtcggcctc gctcattgca tacagcgaac ggttctcgta gtgaatctcc
     7381 ccctgctgcg gcgtcagacg cgcggagatc gacttcagca gcgtggtctt cccggagccg
     7441 gattctccga caatgcccag cacttctccc ggccataaat caaaagaaac atcgctaaag
     7501 cctttgcccg gcgcgtaaag gtgggtcagg ttattgaccg aaagtaacgg tttattcatt
     7561 ggcttttcgc ctcgctctgt tggcggcaat aatcggtatc ggagcagaca aacatgcggt
     7621 ttccggcgtc atccagcacc acttcatcaa gatagctgtg ggtcgatccg cagatggcgc
     7681 atggctcatc ccactgctga acggtgaacg ggtgatcgtc gaaatcgaga ctttccacgc
     7741 gggtaaacgg cggcaccgca tagatgcgct tctcgcgccc cgcgccgaac agttgcaggg
     7801 cgggcatcat gtccattttc gggttatcga atttcgggat cggcgacggg tccattacgt
     7861 agcgcccgtt aaccttcacc ggataggcgt aggtagtggc gatatgaccg aagcgggcaa
     7921 tatcttcata cagtttcacc tgcatcacgc cgtactcctc cagcgcgtgc atggtgcggg
     7981 tttccgtttc gcgcggctcg ataaagcgca gcggctcggg gattggcacc tggaaaataa
     8041 ctatctgatc ttcggtgagc ggcgtttcgg ggatgcgatg gcgcgtctgg ataagcgtcg
     8101 catcgtccgt ccgttcagtg gtgtttaccc cggtcacgcg cttaaagaag ttgcggatcg
     8161 acacggcgtt ggtggtgtcg tctgcgccct ggtcaatcac cttcagcacg tcgctttcgc
     8221 caatcacgct ggcggtgagc tgtatgccgc cggttcccca gccgtatggc atcggcatct
     8281 cgcgcccgcc aaacggcacc tgatagccgg ggatcgccac cgcttttaag atggcgcggc
     8341 ggatcatgcg tttggtctgc tcgtcgaggt acgcaaagtt gtagccgctc agattagcca
     8401 tggttctgct cctgttgcag acgtttgagt agctccagtt cggcctggaa atcgacgtag
     8461 tgcgggagtt tcaggtgcga gacaaagcct gcggcttcga cattgtcggc atgcgccagc
     8521 acgaactctt catcctgcgc cgggcctgtc gcgtgctcgc cgtactctgg agcctgcaac
     8581 gcacgatcga ccagcgccat tgccatcgct ttgcgctcgc tcatgccgaa caccagcccg
     8641 tagccgcgcg tgaagtgcgg ccgctcaccc ggcgggtcga taaaaccgtt gaccatttca
     8701 cactcggtca tcagcagttc gccgacgttc accgcaaatc ccagctcttc cggcacaatc
     8761 gatacgtcga tatagccgct gcggatctcg cctgcgaacg ggtggttgcg accgtagccg
     8821 cgttgggtgg agtaggccag cgccagcaaa tagccttcgt cgccgcgcat caactgctgt
     8881 aaacgggagg agcgtgagca ggggtaaacc ggcggcgtgc gggtgatgtc atccggctgt
     8941 gcgccgctat cctcttcaaa cttcgccagc ccctgacgcg ccagcaggct gaaaacgtgc
     9001 ggcgacggct gttgttcgct gtgggcggtg gtcagcgtcg gcgcttcgcc gtttgccagc
     9061 agggtgaaat cgagcaggcg atgggtgtag tcgtaggttg ggccaagcaa ctggccgccg
     9121 ggaatatctt tataaaccgc tgaaatacgc cgttcgagac gcatctccgt ggtgtcgagc
     9181 ggctcgctta ccgcgagctt cgccagcgtg gtgcggtagg cgcgcagcag gaaaattgct
     9241 tcgacgttat cgccgctggc ctgtttcagc gccagcgccg ccagttcgcg gtcggcaatg
     9301 ccgccttcgg tcatcacgcg atctactgcg aggttaagct gctgttcaat ctgggcgacg
     9361 ctcagttcag gcaaatcggt atcgccccgg cgtcggctct cttgcagggc gtgggcggca
     9421 tcgatcgcct tttcgccccc tttcacggca acgtacatca gcacacctcc acatgcgtgg
     9481 ttcgcggaat agccagcagg cgctcgccgc aggtcaggat caggtcgatg ccgagcggga
     9541 acgggtgcgg gcgctcggtg agttcgtaca gaatgcactc cggcagctgc ggggcgatca
     9601 ttcgttcttc ggcaatgccc gcgccggtga gacgtaacat gcgtccgccg ctcaggctgg
     9661 cgacctgtaa aatcagcgtt gcacccgctt ccggcgcaac ggcggtgccg gtggacaggg
     9721 cgttaagctg ttcgctggaa atcgcctcat ccgttaccgc gaaggtcgcc tgttccggct
     9781 ggctgaccag cggcgcgttg gtatgaaaac gcaggctctg gctgacgatg tcgttactca
     9841 atggcgcgga aagccacacc ggcgtgtcgt tatcggccag cgtcagcagc acgctggtgg
     9901 tggcgatatt cagcggctgc cagccgcgtt tgagctgatg cagggcgaca atcacgcccg
     9961 gctcgctcat ggcctttaac aggcgacgaa aactgtgctg ggcatcctgc acaggaagaa
    10021 taaaagcggt ttccagggtc atgcgttatc tccgcgaacc atcgtaaaga agtcgacccg
    10081 gctggcgttc acttcggcct ggcgtgcggc aatgcgtgcc atacggtcag cgtccagcgg
    10141 ggcaataagg gtttctgata agttttgaaa atagcgagat tgctgcatca gcgcgtcaat
    10201 cagcgcgcag cgttcggcgt gctgtttgtc gcgccccagc acccagctgt agccgagcgt
    10261 gccgtcagtc aggcgtacgg cggcgcgggt gagcgtggcg tcgccggcaa aaaagcgttc
    10321 gccggtgccg cccatccgcg cctgaatctg caccaggccg gtttcggcgg cgcggataac
    10381 ctcatagtcg gcggtgatgt tcagtgcttt caggcgggct gccagttcag ccggttggct
    10441 gtgcgccagt acggacatcc agtgctggcg ggtcgcggta tctgcgtgca ttcagtgctc
    10501 catagtgaat tcaatcatgt cggcgcgcgt caggctgacg gagtactccg ccgggctgct
    10561 ttcaccgtca cggtggttaa gggtgcgcac gcacagcagc ggcgacatat tcggaatttc
    10621 aagacgctgg cactctttgg cctgggcgcg acgggcgctg atccgcgtct ggctgcggcg
    10681 cagcgcaatt ccggtttgct cgcgcagaaa atcgtgcagc gaaccgctgt cgaagcgttg
    10741 cagcgtcggc caaagggtga ggtccgcaaa gtagtggtcg attaaacaga gcgcgacgcc
    10801 gttgacgcga cgcagggtgc gcaaatggat aacgttctcg ccctcggtaa tccccagcgc
    10861 gtcagcgacg tgaccggacg cggggcgcaa taccgacagc agtttttcgc tggtgggatg
    10921 gctaccctgg tccagcaggt tctggctaaa acgcgcctgg gcgttgagcg ggtaatcgaa
    10981 cgggcgcatc agcaccagca cgccgacgcc ctgacggcgc tgtacccagc ctttttccac
    11041 cagttggtcg atggcgcggc gcagggtgtg gcggttcacc tcaaagcgcg ccgccagttg
    11101 ctgctcggcg ggaagatagt cgccgcagcg gtagtgttga cgaagctcct gctcaagttt
    11161 tgcggctatc tcctgatagc gtgttgggta gctggtcgga tgtgtagaca agtgcataga
    11221 tatcaatgcc tcgcttatca gataaagtgc ttacgcaacc gttgagagag gaaatccagc
    11281 aggctgaccg tgacgatgat aagcaccatc agggcgcagg tttgttggaa ctggaaaccg
    11341 cgaatcgctt cccacagggt gacgccgatc ccgcctgcac cgaccatgcc gacgacggtc
    11401 gccgagcgga cgttggattc gaagcgatag agggagtagg agatcagcag cggcatcacc
    11461 tgcggcagca cgccgtagag gatctcttcg agcttgttgg cgccggtggc gcgaatgcct
    11521 tccaccgggc cgggttcaat cgcttccacc gcttcggaaa gcagtttgga gagcacgccg
    11581 gtggtgtgga taaacagcgc cagcacgcca gcgaacgggc cgaggccgac ggcgaccacg
    11641 aacagcatgg cgaagaccat ttcgttaatg gcgcggcagg cgtccatcag gcggcgaacg
    11701 ggctggtaaa cccaccacgg caccaggttt tcggcgctca tcaggccgaa ggggatggag
    11761 agaaccaccg ccagcgccgt gccccagacg gcgatttgca gcgtgacggc catttcggtg
    11821 aggtaatcct gccactggct gaaatcgggc gggaagaagt cggcggcgaa cgtcgccatg
    11881 ttgccgccgt ctttaatcag cgtgagcggg gccatttccg cgccctgcca cgagacgacc
    11941 aacacggcga gaacaacggc ccagctcaga agcgagaacc agctacgctt gggtggggcg
    12001 atggtgatgg tttgcatgtt tggctccggt ctgtaggccg gataaggtgc ttgcaccgca
    12061 tccggcatca acgcctgcac attgtcggat gcgacgccgg ggcgtcttat ccgacctacg
    12121 atttactgca ccgctttact caccgaactc atcgcgctta acgcgttgtt caggcggtcc
    12181 aggtcatcca gctgcgcctg aatctcggtg gtttttgcca gcttgtcctg ctcattcagt
    12241 cctttattgc ttttcacgcc ctgcatctct ttaaacagcg cgagctggcg aatcggcacc
    12301 agttgcaggt cgctggaagc gcggaacggc gcccagccca ggcgttccag caccgctttt
    12361 tcttccggcg ttttgccgta gttcataaag aagtcgtaga tcttgtcttt ggtggtttcg
    12421 gaaaggtttt tacgccagac gatcggatcg cctgggatca gcggcgactt ccagatcacc
    12481 ttcagttctt tcagcttctc tggcgcggag gttttcagct tgtcgaggtt ttcggtgttg
    12541 ttggtggcaa catccacctg cttgttggcg acggccagcg cgttggtttc atgcccagcg
    12601 ttgacggtgc gcttaaagtc gctggcggag atattgtttt tggcaaagac gtagtaaccg
    12661 gggacgagga agccagaggt ggagttagga tcgccattgc cgaaggtgag atctttccgc
    12721 ttcgccagca gatcgttcag gttgttgatc ggactatctt tgttgacgat caacacgctc
    12781 cagtaacctg gcgatccatc cgccgcgacc gtctgggcga agacctggcc gttggcgcga
    12841 tccaccgctt ccattgccga caggttgccg taccaggcga tatccacttt attgaagcgc
    12901 attccctgga taatgcctgc gtagtctggg gcaaagaagg cgttcacctt cacgcccagc
    12961 ttcttctcca tatcctgtaa gaatggcgtc cattgcggtt taaggttttg ctgtgattcc
    13021 gttgaaataa tgccgaaatt cagcgccttt tcctgctctt cggcgtgtgc cgggcttaac
    13081 agggtgctga ggctgaacat gctggtgaag gccagcgagg caattatctt agcgttcatt
    13141 cgttttcctc aatgatgggg atgtcaggca gctttcgcgt tctcttcgat gcgattaatg
    13201 ctgcggtaga gatggtcaaa acgttcgtta tcaaactgtt ggctgctgcc gtcgtagaaa
    13261 acgtgcccct ggcgcagggc gacgatgcgt tcgcagtagc gcagggcgta atccacctga
    13321 tgcagcgtga cgaccacggt gatgccgtcg ttctgattga tgtcgcgcag ggtgtccatc
    13381 acgatgcggg cggattccgg gtccagcgag gcgatgggtt catcggccag aatcaccttc
    13441 gcctgctgca tcagcgcgcg ggcaatcgcc acacgctgct gctgtccgcc ggagagggtg
    13501 gaaacgcgct gatgggcaaa atgcaccatg ccaacgcggg tcagcgcctg taacgcgcgc
    13561 tgtttctgct cgccggtgaa ccagctaaaa caggtgcgcc agaacggcgt gctgccgagc
    13621 gcgccaatca gcacgttctc cagtacgctc aggcggttca ccaggttgaa ttgttggaag
    13681 atgtagccgg tgttggcgcg gcttttgcgg atatcgcgcg ccagacggcc ttcgcgctgg
    13741 actgtgcggc ccagcagctc gatatggctg ccggcggatt tatcgccggt aatcaaaccg
    13801 cttaagtgac gtaaaagggt ggatttgccg gaacccgacg gcccaagcag agccaccatt
    13861 tcaccgtgat gaatgttcag atcaaccgca tgcagcgcct gatgctgatt gaaggttttg
    13921 gcgagcttct cgacacggat aatcgtttgc atgatgcagc ctccctaaaa aagtggctcc
    13981 atcgtggcgg attattgtga cgatttggtt aattaaaagt gactaacaga tgaagagtta
    14041 acgggaattc gatgacagtg cgggagggcg ggcctcccgg cggattattg cgttggttgt
    14101 tgtttgacga cattaatcat ccacggtacg ccaaatttat cggtgacttt gccaaagcca
    14161 tgcgcccaga aagtttcctg ccaggccatt tcgatttttc cgttagcggc aagattgtca
    14221 aaccagcgtt ttccttcttc gacctgttgc gaatcgagca ccagcgtaaa gccggagtag
    14281 ctggcttttc ctgacggcat ggcatcgctc atcatgatgt cgcttccggc aatgcgcacg
    14341 ttggcatgag cgatggcggt atcgggaaat tgcattccgg aagggcagtt ctcggcgctg
    14401 tcctgcgctg attttggcat ttcgccgaag ctgattttat agagcagttc agcgcccaac
    14461 gtacgttgat aataagcaat tgcgtcggca cagttaccgg caaaagagag gtagggactt
    14521 aacggcatga tggtgacctc agttaagaga agccagttaa gtgtagttcg aaatttatac
    14581 agatgagagg gaagcctgat aagacgcggt cagcgtcgca tcaggcagtc ggcatcgttg
    14641 ccggatgcgg cgcgagcgcc ttatccggct taccgatcac aatacaatca gttctttttc
    14701 acaaactcag atttcagttt catcggacca aaaccgtcga ttttgcaatc gatgttatgg
    14761 tcgccttcaa ccaggcggat gtttttcact ttggtgccaa ttttcagcat cgaagagcta
    14821 cctttcacct tcagatcttt aatgatggta acgctgtcgc cgtcagccag cagattgccg
    14881 ttagcatctt taacgatcag ctcgtcgctt tcctgtgcag gttctgcgtc gttccattcg
    14941 taggcacatt ccgggcagat gtacatgccg ttatcttcgt aagtgtattc ggagttgcat
    15001 tttgggcagt gtggtaatga catgtggatt tcctcaaaag tcagcagggc aaaagcgcca
    15061 aaaaacggca gattgccgaa aaaggccgca attatacaca aaatccctga gtttgtcggg
    15121 ggtactgctg attttagacg acgatcgttt tcatttgaaa ggaaacgcaa aa
//
LOCUS       DAPHDW010000106        15019 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.107, whole genome shotgun sequence.
ACCESSION   DAPHDW010000106 DAPHDW010000000
VERSION     DAPHDW010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 15019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15019
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.107"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            132..374
                     /locus_tag="RH057_003261"
     CDS             132..374
                     /locus_tag="RH057_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129590.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAGSAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            488..1126
                     /locus_tag="RH057_003262"
     CDS             488..1126
                     /locus_tag="RH057_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HDV3129591.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVIQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     PVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            1123..1860
                     /locus_tag="RH057_003263"
     CDS             1123..1860
                     /locus_tag="RH057_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HDV3129592.1"
                     /translation="MIKQTIVALILSVGASSVFAAGTVKVFSNGSSEAKTLTGAEYLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAELAADSSADDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISRPGKQP
                     FTPGRDVASYLSGQNLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            1860..3956
                     /locus_tag="RH057_003264"
     CDS             1860..3956
                     /locus_tag="RH057_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HDV3129593.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYNDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETKLAQKRVEPVVPQSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAIGWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(4003..4281)
                     /locus_tag="RH057_003265"
     CDS             complement(4003..4281)
                     /locus_tag="RH057_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129594.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            4550..4960
                     /gene="psiE"
                     /locus_tag="RH057_003266"
     CDS             4550..4960
                     /gene="psiE"
                     /locus_tag="RH057_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HDV3129595.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(5004..6479)
                     /gene="xylE"
                     /locus_tag="RH057_003267"
     CDS             complement(5004..6479)
                     /gene="xylE"
                     /locus_tag="RH057_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HDV3129596.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSSRFGRRDSLKIAAVLFFISGVGS
                     AWPELGFTSINPDNTVPVYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGWRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQAEGILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSILQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQAPGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(6851..7741)
                     /gene="malG"
                     /locus_tag="RH057_003268"
     CDS             complement(6851..7741)
                     /gene="malG"
                     /locus_tag="RH057_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HDV3129597.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            complement(7756..9300)
                     /gene="malF"
                     /locus_tag="RH057_003269"
     CDS             complement(7756..9300)
                     /gene="malF"
                     /locus_tag="RH057_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HDV3129598.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            complement(9454..10644)
                     /gene="malE"
                     /locus_tag="RH057_003270"
     CDS             complement(9454..10644)
                     /gene="malE"
                     /locus_tag="RH057_003270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HDV3129599.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSAGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            11009..12124
                     /gene="malK"
                     /locus_tag="RH057_003271"
     CDS             11009..12124
                     /gene="malK"
                     /locus_tag="RH057_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HDV3129600.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIHIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            12196..13536
                     /gene="lamB"
                     /locus_tag="RH057_003272"
     CDS             12196..13536
                     /gene="lamB"
                     /locus_tag="RH057_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HDV3129601.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            13779..14699
                     /gene="malM"
                     /locus_tag="RH057_003273"
     CDS             13779..14699
                     /gene="malM"
                     /locus_tag="RH057_003273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HDV3129602.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQEPGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTTDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPAPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
BASE COUNT         3711 a         3869 c         3954 g         3485 t
ORIGIN      
        1 tcattccaat tatcctaaaa ccccatctct aattccccgc gccgtaattc gcatgcttta
       61 gttgtgtata ctcgatcccg cccgaaatgt ttttgggtaa atctccattc attcaatgaa
      121 gggaaattgt tatgaaaaaa gttctgtatg gcatttttgc catatctgcg cttgcggcga
      181 cttctgcgtg ggctgcacct gtacaggtgg gcgaagcggc agggtcggca gcaacgtcgg
      241 tttcggcggg gagttcctcc gcgaccagcg tcagcaccgt aagctcggcg gtgggtgtcg
      301 cgctcgcggc aaccggtggc ggtgatggtt ctaataccgg gaccaccaca accacgacca
      361 ccagtaccca gtaataaagt atgtatcccc aaaataattt gagtcattgc atctgtggct
      421 tgaagtatga agggatttac cataaccaca ctccggtgtg gttattctgc ccctctggag
      481 aagagtcgtg aagcgacctg cactcattct tatctgcctg ctattacagg cctgttcagc
      541 cacgactaaa gagctgggca attcactgtg ggacagtctg ttcggcacgc caggcgtaca
      601 actgacggac gatgatattc aaaatatgcc ctacgccagc cagtacatgc agcttaatgg
      661 cgggccgcaa ttgtttgtgg tgctggcctt cgctgaagac ggacaacaaa aatgggtcac
      721 tcaggatcaa gccactcttg tcatacaaca tggtcgtctg gtgaagactt tgcttggcgg
      781 cgacaacctg attgaagtga ataaccttgc cgccgacccg ctgattaaac ccgcacaaat
      841 tgttgatggt gcaacatgga cgcgcacgat gggctggacc gagtaccagc aggtacgcta
      901 cgccaccgca cgctcagtct tcaaatggga tggcaccgat accgtcaagg tcggcagcga
      961 tgaaaccccg gttcgcgtgc tggacgaaga agtctccacc gaccaggcgc gctggcataa
     1021 ccgctactgg atcgacagcg aagggcaaat tcgccagtcg gaacagtatc tcggcgcgga
     1081 ttatttcccg gtgaaaacca ctctcatcaa ggcggcaaaa caatgattaa acaaactatt
     1141 gtcgcattga ttttgagtgt gggggcgtca tcggtcttcg cggcgggaac cgtcaaggta
     1201 ttcagcaatg gcagcagtga ggccaaaacg ctgacgggcg cagagtattt aatcgatctg
     1261 gtaggccagc cgcggctggc aaacagttgg tggcctggtg cggtgattag cgaagagctg
     1321 gcaacggcgg cagcattgcg gcagcagcag gcgttgctga cacggctggc agaactggct
     1381 gcagattcca gcgccgacga tgccgccgcg attaacgcct tacgccagca aattcaggcg
     1441 ttgaaagtga cgggcaggca aaaaatcaat cttgatcccg atatcgtccg cgttgccgaa
     1501 cgcggtaacc caccgttgca gggcaactac acgctgtggg tcgggccacc accgtccacg
     1561 gtcacgctgt tcgggcttat cagccgtcct ggcaagcagc cattcactcc aggtcgcgac
     1621 gtggcgagct atctctccgg gcagaacctg cttagcggtg cggatcgcag ctacgcgtgg
     1681 gtggtttacc cggacggacg cacgcaaaaa gcgccggtgg cttactggaa caagcgtcac
     1741 gtagagccga tgcccggcag cattatttat gttggcctcg cggactccgt ctggagtgag
     1801 acgcctgatg cccttaacgc cgacattctt cagactctga cgcagcggat accccaataa
     1861 tgaaaaaaag acatctgctt agcttactgg cgctgggcat tagcacagct tgctacggcg
     1921 aaacatatcc tgcgcccatt ggtccgtcgc agtcggattt cggtggcgta ggattattac
     1981 aaactcccac cgcgcgtatg gcacgggaag gggagttgag tctgaactat cgcgataacg
     2041 atcagtaccg ttattactca gcttcagtgc aactcttccc gtggctggaa acaacgctgc
     2101 gctacaccga cgtgcgcacc cggcagtaca gcagcgtcga agcgttctct ggcgatcaaa
     2161 cgtataaaga taaagccttc gatctcaaac tgcgtttgtg ggaagagagt tactggctgc
     2221 cgcaagtggc ggttggtgcg cgggatatcg gcggtacggg gctgtttgat gcggaatatc
     2281 ttgttgccag caaagcctgg gggccgttcg attttacgct cggcctgggc tggggatatc
     2341 tgggcaccag cggtaatgta aaaaatccgc tctgttcagc cagtgataaa tattgctatc
     2401 gcgataacag ctacaaacag gcgggatcta tcgacggtag ccagatgttc cacggtcctg
     2461 cctcactgtt tggcggcgtg gaataccaga cgccctggca accgctgcgc ctgaaactgg
     2521 agtatgaagg caataattat cagcaggatt ttgccgggaa gctggagcaa aaaagtaagt
     2581 ttaacgtcgg tgcgatttat cgcgttaccg attgggctga cgttaacctt agctatgaac
     2641 gtggcaacac ctttatgttt ggcgtcacgc tgcgcaccaa ctttaacgat ctgcgcccgt
     2701 cttacaacga taacgcccgc ccgcaatatc aaccgcagcc gcaggatgcc attttgcagc
     2761 attcggtggt ggcgaatcag ttaacgctgt tgaaatacaa tgccggactt gccgatccgc
     2821 agatccaggc gaaaggcgat acgctgtatg tcaccggcga gcaggtgaaa tatcgtgatt
     2881 cgcgcgaagg gatcatccgt gctaatcgga tcgtgatgaa cgatctgccg gatgggatca
     2941 aaacgatccg cattacggaa aatcgtctta acatgccgca ggtgacgacg gaaaccgatg
     3001 tcgccagcct gaaaaatcat ctcgccggag agccgttggg ccacgaaacg aagctggcgc
     3061 aaaaacgcgt cgagccagtg gttccgcagt ccaccgagca gggctggtat atcgacaaat
     3121 cacgctttga tttccacatc gatccggtgc tgaaccagtc ggtcggtggc ccggaaaact
     3181 tttacatgta tcagctgggc gtgatgggaa cggcagattt gtggctgacg gaccatctgc
     3241 tgaccaccgg cagcctgttt gcaaatcttg ccaacaacta cgacaagttt aactacacca
     3301 atccgccgca ggactcgcac ttaccgcgcg ttcgtaccca tgtgcgcgag tatgtgcaga
     3361 acgatgtcta tgtgaataac ctgcaagcca actacttcca gcatctgggc aacggcttct
     3421 acggtcaggt ctatggaggt tatctcgaaa ccatgtttgg cggtgcgggg gccgaagtgt
     3481 tgtatcgtcc gctggacagt aactgggcgt ttggtctgga tgccaactac gttaaacagc
     3541 gcgactggcg tagtgcaaaa gatatgatga aattcaccga ctacagcgtg aaaaccggac
     3601 atctgaccgc ctactggacg ccatctttcg cccaggacgt gttggtgaaa gccagcgtcg
     3661 ggcagtatct ggcaggggat aaaggcggca cgctggagat cgccaaacgc tttgatagcg
     3721 gcgtggtggt tggcggctat gccacgatca ccaacgtttc gaaagaggag tacggcgaag
     3781 gggacttcac caaaggcgtg tatgtctcgg taccgttgga tctcttctcg tctggcccga
     3841 cacgcagccg tgcggcgatt ggctggacgc cgctgacgcg tgacggtggt cagcaacttg
     3901 ggcgtaagtt ccagttgtat gacatgacca gcgaccgtag cgtcaatttc cgctaagtca
     3961 tgggaaaggt gccagttttc gcaatcactg gcaccctcat tcttaatggc ctgttgagcc
     4021 atcaggaacg cagttcaccc ccggtttgcg atctggtgtg taaaggcgaa aacattctgt
     4081 cttcctgtcc gtcgatatcg gttgcagatc tggttgcctg agcgactggg ccatcattgg
     4141 gctggagaca ttgctttgcg gtaggtcggc tttatcagca gtaccgagcg gaccagcata
     4201 agcaggaaga acagagactg ataacatcaa agcagcaaaa taaggcttca tttttaccac
     4261 ctttatcagg ttacgtttca tttgttccag aggaacatgg tcgatttttc gcgcattgct
     4321 ggtggctggg aatcacctga atgggtgatt ttgaattacc ggctttggtg cggtttgtct
     4381 tgccggatgc gccgccaggc gcggcttatc cggcctacgg gtaggtatat ccggctttgg
     4441 tggaggcgcg ctccaaatcc aggttgaaca aaacatacac aaaaaatata gatctccgtc
     4501 acatttttgc gttatacagg aagctcgcca ctgtgaagga ggtactgcta tgacgtcact
     4561 ctctcgtccg cgcgtggagt ttatctccac cattttgcag accgtactca atcttggcct
     4621 gttgtgcctc ggcctgattt tggttgtctt cctcggcaaa gaaacggtgc atctggctga
     4681 tgtgctattc gcgccagaac aaaccagcaa atatgagctg gtagaaggac tggtggttta
     4741 ctttctctat ttcgaattta tcgcgctgat tgtgaagtac tttcagtccg gttttcactt
     4801 cccgctacgt tactttgtct atattgggat caccgccatt gtgcgcttga tcatcgtcga
     4861 tcataaatcg ccactggatg tgttgatcta ctcggctgcg atcctgttac tggtgatcac
     4921 cctgtggctg tgtaattcga aacggctgaa gcgggagtaa aaagacagca cgccgaactg
     4981 gcgcggcgtg ctggacagga agattacagc gtagcagttt gttgtgtttt cttcgtttcc
     5041 ggttcccaga gggcttccag ctcctcaagg gttttacctt tggtttccgg gacaaatttc
     5101 cacataaata gtgctgccag aacgcccata cacccgtaaa tccagtagga gaaaccgttg
     5161 tggaaatggg ccaccagcca ggagtttttg tccatcatcg ggaaggtcca ggagacgaag
     5221 tagttcgcca gccactgggc cgccaccgcg attgccagcg ctttaccacg aatagcattc
     5281 gggaagattt ccgacagcag tacccagcat accggacccc aggacatggc aaaggcggca
     5341 acatagaaca gcatcgacag aagcgccaca atacccggtg cctgagtgta aaacgcggta
     5401 ccgaggctaa acataccgat tgccattccg agtgcgccga taatttgcag tggcttacga
     5461 ccaaatttat ccaccgtcat aattgccaga acggtgaagg tgaggttgat aactccgaca
     5521 ataatggtct gcaacagcgc gatatccgtg ctggccccca gcgttttgaa cacttccggc
     5581 gcgtagtaca gcaccacatt gatgccgaca aattgctgga ggatggagag cataacgccg
     5641 atgacaatca cgcctacgcc aaacatcagc agacgaccac cggttttgcg gccatgatcc
     5701 agagagtgtt taatttcctg tactgcctga gttgcaaggg tgttgcccat aattttgcgc
     5761 aggatacctt ccgcctgttc ttgcttgccg cgtgacatca gccagcgagg actttctggc
     5821 acggtataca gcagcattaa gaacagcagt gcagggatac attccgaggc aaacatataa
     5881 cgccagccgt cagtattcag ccagctggcg tcgccggaac gggcaataaa atagtttacg
     5941 cagtaaacta aaagctgccc gaaaataatc gcaaactggt taaaagagac cagtttcccg
     6001 cgaatatgag caggagcaag ttccgcaata tacattggtg aaagcattga ggctaaacca
     6061 acgccaatac cgccaataat gcgataaata acaaattccg ggacataacc tgccagataa
     6121 acaggcactg tgttgtccgg gtttatagag gtaaaaccaa gttctggcca ggcagaacct
     6181 acaccagaaa taaaaaacag gacagcagca atcttaagtg aatcacgacg accgaagcgg
     6241 ctactgcaat aaccaccgag ggcaccgccg atgatgcaac caatcagagc gctggccacg
     6301 caaaacccta acagggagtt ggcagcggat tcacttaagt tttgtggagc aacaaagacg
     6361 gtattgagtg actcaacagt accggaaata acggcggtgt cgtagccaaa taataaacca
     6421 cctaatgtag cgactaaggt aatcgaaaat atataactgg aattatactg ggtattcatt
     6481 cagacctgcc ttagaccatt ctgatctttt atcagtgcat tgatgttgga attgtcaggc
     6541 agttaaggca attatcattt tttacaatgc gtttacgtaa tgtcttttct gtgatcttaa
     6601 ttgtgataat tatcaaaaat tgatgaaaac aatactattg ccgtgactca gagcacgaaa
     6661 gagaattatc gtaagtggga aaacaaataa cgtaaaaata ataagttcta tgatggaaat
     6721 attaaccggc gaacgattca gattgcagac gaaagaaaaa aaggcgctcc gtggagcgcc
     6781 gaataacagt cacaagttgg gataacttaa gttgagggtg cagcggcata acattggcag
     6841 aacaacatct ttaacctttc acaccacctg ccgtcaggcc gttgaccagc cagcgttgag
     6901 ccagcaagaa gacgatggtg atcggtaatg cagacatcac ggcagcggcg gcaaagtcac
     6961 cccacaggta gttttgcggg ttgaggtatt gctgcatccc cacggccagg gtgtagctgt
     7021 ttacgtcacg cagtaacagc gacgcgaccg gaacttcagt aatggcagcg ataaacgaca
     7081 ggatgaacac caccgccaga atcggtactg acagcggcaa caggacaagg cggaaggcct
     7141 gccacggtgt cgcaccatcc agcgccgccg cttcttccag cgaactgtcg atggtttcga
     7201 aatagccttt gatggtccag acgtgcagcg caatcccacc cagatacgcg aaaattacgc
     7261 cgccgtgagt attcaggcca atgaatggaa tgtactcacc cagacgatca aacaacgcat
     7321 acaacgcgac cagtgaaagt actgccggga acatctggaa aatcagcatt cctttcagca
     7381 gcgtcgcttt gcctggaaag cgcatacggg cgaaagcgta ggcgcaggtg gtggagagcg
     7441 ccacaatgcc aatcgcggaa atcccggcga cctttaccga gttccacagc cacagcagta
     7501 ccgggaatgg cggtggcgta atgcgaccat cagcctgttc cacgctaaaa cctaatgcca
     7561 gtttccagtg atcccaggag atttgctccg ggatcaggct accggtcgca aagttcccct
     7621 ggcgcagtga gatggcgacg accatcagca gcgggaacat aatcgccgcg ataaaaagta
     7681 gcagtagcag gtgagtaata aacaaacgtg ctttttgcga tttcggttgg accattgcca
     7741 tttttgttat ctcccttaat caaacttcat tcgcgtggct ttcaggttca ctatcgccag
     7801 cgcacccacc agcaggaaga tcagcgtggc tattgctgct gccagaccga agtcctgacc
     7861 cccgccgcct tcaaaagcga tgcggtaggt gtagttaaca agcaggtcgg tataaccggc
     7921 tggcgtggtc gtgccaagac gatccgggcc gccgttggtt aacagttgaa tcagcacgaa
     7981 gttgttaaag ttaaaggcga agctggcgat catcagcggc gtcagcggtt taatcagcag
     8041 cggcagcgta atcttaaaga agttctggaa gggacctgca ccatccattg ctgaggcttc
     8101 atacaaatcg tccggaatcg ctttcagcaa gcccatgcag aggatcatca tgtacggata
     8161 acccagccag gtattgacga taatcagcat cgttcgggcg gtggtcggat cgctgaacca
     8221 ggcgggcttc acgccaaaca gcgcgctcaa catcatgttg atctcaccga agctctggtt
     8281 aaacaatcct ttgaaaatca agattgaaat gaacgatggc accgcgtagg gcagaatcag
     8341 caggacgcga tagaccgctt tgccgcgcaa cgcttcccac tgcaccagac atgccagaac
     8401 catgccgacc gccaccgtta aaaagacagt gatcagcgag aacaccacgg tccagacgaa
     8461 aatggcgagg aacggtttct gaatgccttc gtcggtaaag acgcgggtaa agtttttcca
     8521 gccggtggtc acggtgtaac cggggcttag cttttcatca ccccagttgc cgtcggcggt
     8581 aatggactgg taaaagccaa tttggttatt cggacgatat ttcacgccgc tctgattatt
     8641 cgtcaacgtg ccgtcaccgt cgagtgtgta gagcggctgc gtgccagaaa actggcgcag
     8701 ggagctcatc atcactttgt tgccatccgg cagaatggcg gtaatgtcac tcagcgcctg
     8761 acgattctgg gtaatcacgc gtagattcgc gcgttcgcct tcgggctggg cggtcgtttc
     8821 tttcagttgc agtttttgct cgccgccaaa tttaaaagcg tcggaaaggt aatttttgcc
     8881 ggtttcgccg tcactgagcg ccagttgcca ctcatcgccc gccgggtaaa gaccaaagtt
     8941 ataggttttg cctgcttgcc aggagcgatc taacaacact tcctgtgcac gttcaaaagt
     9001 cagctggtta gtgctgctgt agttggtgaa ggcaatggcg atggtgcaga ccagagggaa
     9061 gaggacgaat aatcccattc cagccattcc cgggtaaaca tagcgccagg cgtaggcttt
     9121 acgattggcg aaaatataca gccccgctga actcaatatc agcgtggtaa tggcgaacag
     9181 gtattcccct tgtgcgtaca ttaaaacaac aaggtaaccc accagcaggc cgagcagacc
     9241 tagcactgac catttcagcg cgtcgctttg ccaccaatgt ttctttttaa tgacatccat
     9301 ggggttcttc ctcattccag gacggataag gctttcacgc cttatccgac aacaactgcc
     9361 tgatgcgaca ctgatgcgtc ttatcaggcc tacatgcgtt tcagttttgt aggccggaca
     9421 aggcgttcac gccgcatccg gcatttcaca gcattacttg gtgatacgag tctgcgcgtc
     9481 tttcagggct tcatcgacag tctgacgacc gctggcggcg ttgatcaccg cagtacgcac
     9541 ggcataccag aaagcggaca tctgcgggat gttcggcatg atttcacctt tctgggcgtt
     9601 ttccatggtg gcggcaatac gtggatcttt cgccaactct tcctcgtaag acttcagcgc
     9661 cactgcaccc agcggtttgt ctttattgac cgcttccaga ccttcgtcag tcagcagata
     9721 gttttcgagg aactctttcg ccagctcttt gttcggactg gcggcgttaa taccggcgct
     9781 cagcacgcca acgaacggtt tagatggttg acccttgaag gtcggcagta ccgttacacc
     9841 ataattcact ttgctggtgt cgatgttgga ccatgcccac gggccgttga tggtcatcgc
     9901 tgtttcgcct ttattaaagg cagcttctgc gatggagtaa tcggtgtctg cattcatgtg
     9961 tttgttttta ataaggtcaa ccaggaaggt cagacccgct ttcgcgccag cgttatccac
    10021 gcccacgtct ttaatatcgt acttgccgtt ttcatacttg aacgcataac ccccgtcagc
    10081 agcaatcagc ggccaggtga agtacggttc ttgcaggttg aacatcagcg cgctcttacc
    10141 tttcgctttc agttctttat ccagcgccgg gatctcttcc caggtttttg gtgggttcgg
    10201 cagcagatct ttgttataaa tcagcgataa cgcttcaaca gcgatcgggt aagcaatcag
    10261 cttgccgttg taacgtacgg catcccaggt aaacggatac agcttgtcct ggaacgcttt
    10321 gtccggggtg atttcagcca acaggccaga ttgagcgtag ccaccaaagc ggtcgtgtgc
    10381 ccagaagata atgtcagggc catcgccagt tgccgcaacc tgagggaatt tctcttccag
    10441 tttatccgga tgctcaacgg tgactttaat tccggtatct ttctcgaatt tcttaccgac
    10501 ttcagcgaga ccgttatagc ctttatcgcc gttaatccag attaccagtt taccttcttc
    10561 gattttggcg agagccgagg cggaaaacat catcgtcgtt aatgcggata atgcgaggat
    10621 gcgtgcacct gtttttattt tcataatcta tggtccttgt tggtgaagtg ctcgtgaaaa
    10681 cacctaaacg gactctagtt tctttatacg gcaacctctt tccatcctcc ttgcccctac
    10741 gccccaccgt tgctttgtgt gatctctgtt acagaattgg cggtaatgtg gagatgcgca
    10801 cataaaatcg ccacgatttt tgcaagcaac atcacgaaat tccttacatg acctcggttt
    10861 agttcacaga agccgtgttc tcatcctccc gcctcctccc ccataaaaaa gccagggggt
    10921 ggaggattta agccatctcc tgatgacgca tagtcagccc atcatgaatg ttgctgtcga
    10981 tgacaggttg ttacaaaggg agaagggcat ggcgagcgta cagctgcaaa atgtaacgaa
    11041 agcctggggc gaggtcgtgg tatcgaaaga tatcaatcta gatatccatg aaggtgaatt
    11101 cgtggtgttt gtcggaccgt ctggctgcgg taaatcgact ttactgcgca tgattgccgg
    11161 gcttgagacg atcaccagcg gcgacctgtt catcggtgag aaacggatga atgacactcc
    11221 gccagcagaa cgcggcgttg gtatggtgtt tcagtcttac gcgctctatc cccacctgtc
    11281 agtagcagaa aacatgtcat ttggcctgaa actggctggc gcaaaaaaag aggtgattaa
    11341 ccaacgcgtc aaccaggtgg cggaagtgct gcaactggcg catttgctgg atcgcaaacc
    11401 gaaagcgctc tctggcggtc agcgtcagcg tgtggcgatt ggccgtacgc tggtggccga
    11461 gccaagcgta tttttgctcg atgaaccgct ctccaacctc gatgctgcac tgcgtgtgca
    11521 aatgcgtatc gaaatctccc gtctgcataa acgcctgggc cgcacaatga tttacgtcac
    11581 ccacgatcag gtcgaagcga tgacgctggc cgacaaaatc gtggtgctgg acgccggtcg
    11641 cgtggcgcag gttgggaaac cgctggagct gtaccactat ccggcagacc gttttgtcgc
    11701 cggatttatc ggttcgccaa agatgaactt cctgccggta aaagtgaccg ccaccgcaat
    11761 cgatcaagtg caggtggagc tgccgatgcc aaatcgtcag caagtctggc tgccagttga
    11821 aagccgtgat gtccaggttg gagccaatat gtcgctgggt attcgcccgg aacatctact
    11881 gccgagtgat atcgctgacg tcatccttga gggtgaagtt caggtcgtcg agcaactcgg
    11941 caacgaaacc caaatccata tccagatccc ttccattcgt caaaacctgg tgtaccgcca
    12001 gaacgacgtg gtgttggtag aagaaggtgc cacattcgct atcggcctgc cgccagagcg
    12061 ttgccatctg ttccgtgagg atggcactgc atgtcgtcga ctgcataagg agccgggcgt
    12121 ttaagcaccc cacaaaacac acaaagcctg tcacaggtga tgtgaaaaaa gaaaagcaat
    12181 gactcaggag atagaatgat gattactctg cgcaaacttc ctctggcggt tgccgtcgca
    12241 gcgggcgtaa tgtctgctca ggcaatggct gttgatttcc acggctatgc acgttccggt
    12301 attggctgga caggtagcgg cggtgaacaa cagtgtttcc agactaccgg tgctcaaagt
    12361 aaataccgtc ttggcaacga atgtgaaact tatgctgaat taaaattggg tcaggaagtg
    12421 tggaaagagg gcgataagag cttctatttc gacactaacg tggcctattc cgtcgcgcaa
    12481 cagaatgact gggaagctac cgatccggcc ttccgtgaag caaacgtgca gggtaaaaac
    12541 ctgatcgaat ggctgccagg ttccaccatc tgggcaggta agcgcttcta ccaacgtcat
    12601 gacgttcata tgatcgactt ctactactgg gatatttctg gtcctggtgc cggtctggaa
    12661 aacatcgatg ttggcttcgg taaactctct ctggcagcaa cccgctcctc tgaagctggt
    12721 ggttcttcct cttttgccag caacaatatt tatgactata ccaacgaaac cgcgaacgac
    12781 gttttcgatg tgcgtttagc gcagatggaa atcaacccgg gcggcacatt agaactgggt
    12841 gtcgactacg gtcgtgccaa cctgcgtgat aactatcgtc tggttgatgg cgcatcgaaa
    12901 gacggctggt tattcactgc tgaacatact cagagtgtcc tgaagggctt taacaagttt
    12961 gttgttcagt acgctactga ctcgatgacc tcgcagggta aaggtctgtc gcagggttct
    13021 ggcgtcgcgt ttgataacga aaaatttgcc tacaatatca acaacaacgg tcacatgctg
    13081 cgtatcctcg accacggtgc gatctccatg ggcgacaact gggacatgat gtacgtgggt
    13141 atgtaccagg atatcaactg ggataacgac aacggcacca agtggtggac cgttggtatt
    13201 cgcccgatgt acaagtggac gccaatcatg agcaccgtga tggaaatcgg ctacgacaac
    13261 gtcgaatccc agcgcaccgg cgacaagaac aatcagtaca aaattaccct cgcacaacaa
    13321 tggcaggctg gcgacagcat ctggtcacgc ccggctattc gtgtcttcgc aacctacgcc
    13381 aagtgggatg agaaatgggg ttatgactac aacggcgata gcaaggttaa cccgaactac
    13441 ggcaaagccg ttcctgctga tttcaacggc ggcagcttcg gtcgtggcga cagcgacgag
    13501 tggaccttcg gtgcccagat ggaaatctgg tggtaatagc aaaacctggg ccggataagg
    13561 cgtttacgcc gcattcgaca accaacgcct gatgcgacgc ttgcgcgtct tatcaggcct
    13621 acaacggctg tcaaatgtag gccggataag gcgtttacgc cgcatccggc ataaaaacag
    13681 gttgtcatta tctgaaaggg gcgaaagccc ctctgattat cgggtttagc gcgctattgc
    13741 ctggctaccg ctgagctcca gattttgagg tgaaaacaat gaaaatgaat aaaagtctca
    13801 tcgccctctg tttatcagca gggttactgg caagcgcgcc tggaatcagc cttgccgatg
    13861 ttaactacgt accgcaaaac accagcgacg cgccagccat tccatctgct gcgctgcaac
    13921 aactcacctg gacaccggtc gatcaatcta aaacccagac cacccaactg gcgaccggcg
    13981 gccaacaact gaacgttccc ggcatcagtg gtccggttgc tgcgtacagc gtcccggcaa
    14041 acattggcga actgaccctg acgctgacca gcgaagtgaa caaacaaacc agcgtttttg
    14101 cgccgaacgt gctgattctt gatcagaaca tgaccccatc agccttcttc cccagcagtt
    14161 atttcaccta ccaggaacca ggcgtgatga gtgcagatcg gctggaaggc gttatgcgcc
    14221 tgacaccggc gttggggcag caaaaacttt atgttctggt ctttaccacg gaaaaagatc
    14281 tccagcagac gacccaactg ctcgacccgg ctaaagccta tgccaagggc gtcggtaact
    14341 cgatcccgga tatccccgat ccggttgctc gtcataccac cgatggctta ctgaaactga
    14401 aagtgaaaac gaactccagc tccagcgtgt tggtaggacc tttatttggt tcttccgctc
    14461 cagctccggt tacggtaggt aacacggcgg caccagctgt ggctgcaccc gctccggcac
    14521 cggtgaagaa aagcgagccg atgctcaacg acacggaaag ttattttaat accgcgatca
    14581 aaaacgctgt cgcgaaaggt gatgttgata aggcgttaaa actgcttgat gaagctgaac
    14641 gcctgggatc gacatctgcc cgttccacct ttatcagcag tgtaaaaggc aaggggtaat
    14701 tacgccccac agtgctgatt ttgcaacaac tggtgcgtct cctggcgcac ctttttttat
    14761 gcttccttcc tgggatatga gcgatttttt atagtaactc acttcttctt cactaagaat
    14821 atccattatc tcaatgcctt atcagagatt cttttccttt cgccggtagt gtctggacat
    14881 tcaggctact tttccaggtt attttatttc tgttatgcag aggttttatg ataagtcata
    14941 tcctaaattc tggcggcaat aactctttga tgaaacatga tgtggtgcaa ggaaataata
    15001 tagtagatct tgatttact
//
LOCUS       DAPHDW010000107        14859 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.108, whole genome shotgun sequence.
ACCESSION   DAPHDW010000107 DAPHDW010000000
VERSION     DAPHDW010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.108"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..701
                     /locus_tag="RH057_003274"
     CDS             <1..701
                     /locus_tag="RH057_003274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV3129603.1"
                     /translation="GIDIDMHIDAASGGFLAPFVAPDIVWDFRLPRVKSISASGHKFG
                     LAPLGCGWVIWRDEEALPQELVFNVDYLGGQIGTFAINFSRPAGQVIAQYYEFLRLGR
                     EGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVCFKLKDGEDPGYTLYD
                     LSERLRLRGWQVPAFTLGGEATDIVVMRIMCRRGFEMDFAELLLEDYKASLKYLSDHP
                     KLQGIAQQNSFKHT"
     gene            857..2392
                     /gene="gadC"
                     /locus_tag="RH057_003275"
     CDS             857..2392
                     /gene="gadC"
                     /locus_tag="RH057_003275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HDV3129604.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            2523..3842
                     /locus_tag="RH057_003276"
     CDS             2523..3842
                     /locus_tag="RH057_003276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HDV3129605.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            4214..5596
                     /gene="dosC"
                     /locus_tag="RH057_003277"
     CDS             4214..5596
                     /gene="dosC"
                     /locus_tag="RH057_003277"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HDV3129606.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPFIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRNLNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSQAFYDNVRSSDYVFRYGGDEFIIVLTEASENETLR
                     TAERIRSRVEKTKLKAANGEDIALSLSIGAAMFNGHPDYERLIQIADEALYIAKRRGR
                     NRVELWKASL"
     gene            5621..8020
                     /gene="dosP"
                     /locus_tag="RH057_003278"
                     /gene_synonym="pdeO"
     CDS             5621..8020
                     /gene="dosP"
                     /locus_tag="RH057_003278"
                     /gene_synonym="pdeO"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-sensing cyclic-di-GMP phosphodiesterase"
                     /protein_id="HDV3129607.1"
                     /translation="MKLTDADNAADGIFFPALEQNMMGAVLINENDEVMFFNPAAEKL
                     WGYKREEVIGNNIDMLIPRDLRPAHPEYIRHNREGGKARVEGMSRELQLEKKDGSKIW
                     TRFALSKVSAEGKVYYLALVRDASVEMAQKEQTRQLIIAVDHLDRPVIVLDPERHIVQ
                     CNRAFTEMFGYCISEASGMQPDTLLNIPEFPADNRIRLQQLLWKTARDQDEFLLLTRT
                     GEKIWIKASISPVYDVLAHLQNLVMTFSDITEERQIRQLEGNILAAMCSSPPFHEMGE
                     IICRNIESVLNESHVSLFALRNGMPIHWASSSHGAEVQNAQSWSATIRQRDGAPAGIL
                     QIKTSSGAETSAFIERVADISQHMAALALEQEKSRQHIEQLIQFDPMTGLPNRNNLHN
                     YLDDLVDKAVSPVVYLIGVDHIQDVIDSLGYAWADQALLEVVNRFREKLKPDQYLCRI
                     EGTQFVLVSLENDVSNITQIADELRNVVSKPIMIDDKPFPLTLSIGISYDVGKNRDYL
                     LSTAHNAMDYIRKNGGNGWQFFSPAMNEMVKERLVLGAALKEAISNNQLKLVYQPQIF
                     AETGELYGIEALARWHDPQHGHVPPSRFIPLAEEIGEIENIGRWVIAEACRQLAEWRS
                     QNIHIPALSVNLSALHFRSNQLPNQVSDAMHAWGIDGHQLTVEITESMMMEHDTEIFK
                     RIQILRDMGVGLSVDDFGTGFSGLSRLVSLPVTEIKIDKSFVDRCLTEKRILALLEAI
                     TSIGQSLNLTVVAEGVETKEQFEMLRKIHCRVIQGYFFSRPLPAEEIPGWMSSVLPLK
                     I"
     gene            8278..8859
                     /gene="ddpX"
                     /locus_tag="RH057_003279"
     CDS             8278..8859
                     /gene="ddpX"
                     /locus_tag="RH057_003279"
                     /EC_number="3.4.13.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
                     /protein_id="HDV3129608.1"
                     /translation="MSDTTELVDLAVIFPDLEIELKYACADNITGKAIYQQARCLLHK
                     DAITALAKSISIAQLSGLQLVIYDAYRPQQAQAMLWQACPDPQYVVDVTVGSNHSRGT
                     AIDLTLRDEHGNILDMGAGFDEMHERSHAYHPSVPPAAQRNRLLLNAIMTGGGFVGIS
                     SEWWHFELPQAASYPLLADQFTCFISPGTQHVS"
     gene            8873..10423
                     /locus_tag="RH057_003280"
     CDS             8873..10423
                     /locus_tag="RH057_003280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3129609.1"
                     /translation="MKRSIPFRPTLLALVLATNFPVAHAAVPKDMLVIGKAADPQTLD
                     PAVTIDNNDWTVTYPSYQRLVQYKTDGDKGSTDVEGDLASSWKASDDQKEWTFTLKDN
                     AKFADGTPVTAEAVKLSFERLLKIGQGPAEAFPKDLKIDAPDEHTVKFTLSQPFAPFL
                     YTLANDGASIINPAVLKEHAADDARGFLAQNTAGSGPFMLKSWQKGQQLVLVPNPHYP
                     GNKPNFKRVSVKIIGESASRRLQLSRGDIDIADALPVDQLNALKQENKVNVAEYPSLR
                     VTYLYLNNSKAPLNQADLRRAISWSTDYQGMVNGILSGNGKQMRGPIPEGMWGYDATA
                     MQYNHDETKAKAEWDKVTSKPTSLTFLYSDNDPNWEPIALATQSSLNKLGINVKLEKL
                     ANATMRDRVGKGDYDIAIGNWSPDFADPYMFMNYWFESDKKGLPGNRSFYENSEVDKL
                     LRNALATTDQTQRTRDYQQAQKIVIDDAAYVYLFQKNYQLAMNKEVKGFVFNPMLEQV
                     FNINTMSK"
     gene            10425..11447
                     /locus_tag="RH057_003281"
     CDS             10425..11447
                     /locus_tag="RH057_003281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3129610.1"
                     /translation="MTFWSILRQRCWGLVLVVAGVCVITFIISHLIPGDPARLLGGDR
                     ASDAIVENIRQQLGLDQPLYVQFYRYVSDLFHGDLGTSIRTGRPVLEELRIFFPATLE
                     LAFCALLLALLIGIPLGILSAVWRNRWLDHLVRIMAITGISTPAFWLGLGVIVLFYGH
                     LQILPGGGRLDDWLDPPTHVTGFYLLDALLEGNGEVFFNALQHLILPALTLAFVHLGI
                     VARQIRSAMLEQLSEDYIRTARASGLPGWYIVLCYALPNALIPSITVLGLALGDLLYG
                     AVLTETVFAWPGMGAWVVTSIQALDFPAVMGFAVVVSFAYVLVNLVVDLLYLWIDPRI
                     GRGGGE"
     gene            11444..12340
                     /locus_tag="RH057_003282"
     CDS             11444..12340
                     /locus_tag="RH057_003282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HDV3129611.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLML
                     LMMNFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTAV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            12337..13323
                     /locus_tag="RH057_003283"
     CDS             12337..13323
                     /locus_tag="RH057_003283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3129612.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRSEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIALAFSCEPQLIIADEPTTALDVTLQLQVLRLLKHKARASGTAVLFISHDMV
                     VVSQLCDRVYVMYAGSVIESGVTADIIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPEGCAFRDRCYAAGAQCENVPALTACGDNNQRCACWYPQQEVISV"
     gene            13316..14242
                     /locus_tag="RH057_003284"
     CDS             13316..14242
                     /locus_tag="RH057_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3129613.1"
                     /translation="MSNTLLTLCDVHINFPARKNWLGKTTEHVHAINGIDLQIRRGET
                     LGIVGESGCGKSTLAQLLMGMLQPSHGQYIRSGSQRIMQMVFQDPLSSLNPRLPVWRI
                     ITEPLWIAKRSSEQQRRALAEELAVQVGIRPEYLDRLPHAFSGGQRQRIAIARALSSQ
                     PDVIVLDEPTSALDISVQAQILNLLVTLQVNRGLTYVLISHNVSVIRHMSDRVAVMYL
                     GQIVELGDAQQVLTAPAHPYTRLLLDSLPAIDKPLEEEWALRKTDLPGNRTLPQGCFF
                     YERCPLATHGCEVRQSLTIREDGREIRCWRAL"
     gene            complement(14298..14729)
                     /gene="osmC"
                     /locus_tag="RH057_003285"
     CDS             complement(14298..14729)
                     /gene="osmC"
                     /locus_tag="RH057_003285"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HDV3129614.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVLLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         3623 a         3717 c         3783 g         3736 t
ORIGIN      
        1 ccggtatcga catcgacatg cacatcgacg ctgccagcgg tggcttcctg gcaccgttcg
       61 tcgccccgga tatcgtctgg gacttccgcc tgccgcgtgt gaaatcgatc agtgcttcag
      121 gccataaatt cggtctggct ccgctgggct gcggctgggt tatctggcgt gacgaagaag
      181 cgctgccgca ggaactggtg ttcaacgttg actacctggg tggtcaaatt ggtactttcg
      241 ccatcaactt ctcccgcccg gcgggtcagg taattgcaca gtactatgaa ttcctgcgcc
      301 tcggtcgtga aggctacacc aaagtacaga acgcctctta ccaggttgcc gcttatctgg
      361 cggatgaaat cgccaaactg gggccgtatg agttcatctg tacgggtcgc ccggacgaag
      421 gcatcccggc ggtttgcttc aaactgaaag atggtgaaga tccgggatac accctgtacg
      481 acctctctga acgtctgcgt ctgcgcggct ggcaggttcc ggccttcact ctcggcggtg
      541 aagccaccga catcgtggtg atgcgcatta tgtgtcgtcg cggcttcgaa atggactttg
      601 ctgaactgtt gctggaagac tataaagcct ccctgaaata tctcagcgat cacccgaaac
      661 tgcagggtat tgcccagcag aacagcttta aacatacctg ataacgttta acggtaacgg
      721 tgtcccgaaa cgaacccgtt tcgggacaat ttccaaagtc tgttcactgg cattagcaac
      781 ggaaaatatt gttctgaata cgcttcagaa caaaacaggt gcggttccga caggaatacc
      841 gttttagggg gataatatgg ctacatcagt acagacaggt aaagctaagc agctcacatt
      901 actcggattc tttgccataa cggcatcgat ggtaatggct gtttatgaat accctacctt
      961 cgcaacatcg ggcttttcat tagtcttctt cctgctatta ggcgggattt tatggtttat
     1021 tcccgtggga ctttgtgctg cggaaatggc caccgtcgac ggctgggaag aaggtggtgt
     1081 cttcgcctgg gtatcaaata ctctggggcc gagatgggga tttgcagcga tctcatttgg
     1141 ctatctgcaa atcgccattg gttttattcc gatgctctat ttcgtgttag gggcactctc
     1201 ctacatcctg aaatggccag cgctgaatga agaccccatt accaaaacta ttgcagcgct
     1261 catcattctt tgggcgctgg cattaacgca gtttggtggc acgaaataca cggcgcgaat
     1321 tgctaaagtt ggcttcttcg ccggtatcct gttacctgca tttattttga tcgcattagc
     1381 ggctatttat ctgcactccg gtgcccccgt tgctatcgaa atggattcga agaccttctt
     1441 ccctgacttc tctaaagtgg gcaccctggt tgtatttgtt gccttcattt tgagttatat
     1501 gggcgtagaa gcttccgcaa cccacgtcaa tgaaatgagt aacccagggc gcgactatcc
     1561 actggctatg ttactgctga tggtggcggc aatctgctta agctctgttg gcggtttatc
     1621 tattgcgatg gtcattccgg gtaatgaaat caacctctcc gcaggtgtaa tgcaaacctt
     1681 taccgttctg atgtcccatg tggcaccgga aattgagtgg acggttcgcg tgatctccgc
     1741 actgctgttg ctgggtgttc tggcggaaat cgcctcctgg attgttggtc cttctcgcgg
     1801 gatgtatgtc acagcgcaga aaaacctgct gccagcggca ttcgctaaaa tgaacaaaaa
     1861 tggcgtaccg gtaacgctgg tcatttcgca gctggtgatt acttctatcg cgttgatcat
     1921 cctcaccaat accggtggcg gtaacaacat gtccttcctg atcgctctgg cgctgacggt
     1981 ggtgatttat ctgtgtgctt atttcatgct gtttattggc tacattgtgt tggttcttaa
     2041 acatcctgac ttaaaacgca catttaatat ccctggtggt aaaggggtga aactggtcgt
     2101 ggcaattgtc ggtctgctga cttcaattat ggcgtttatt gtttccttcc tgccgccgga
     2161 taacatccag ggtgattcta ccgatatgta tgttgaatta ctggttgtta gtttcctggt
     2221 ggtacttgcc ctgcccttta ttctctatgc tgttcatgat cgtaaaggca aagcgaatac
     2281 cggcgtcact ctggagccaa tcaacagtca gaacgcacca aaaggtcact tcttcctgca
     2341 cccgcgtgca cgttcaccac actatattgt gatgaatgac aagaaacact aaccatgtct
     2401 gaatggttat aagacaaggg agcgataagt catcgctccc tttttcgtgc ttgctgtcgt
     2461 attgacttaa ccgggataag tacgagaatg agcgcacatc tgtttaccgg aaaccagcac
     2521 atatggatat ctgctcccga aacaagaaat taacgattag aagaccagcg atactagttg
     2581 cactggcact tttactgtgt agttgtaaaa gcacgcctcc agagtccatg gtgacaccac
     2641 cagcaggttc aaagccacca gccacgacgc aacaatcgtc acaaccgatg cgtggcatct
     2701 ggctggccac ggtttctcgg ctcgactggc caccggtttc ctcggttaac attagtaacc
     2761 ccaccagccg ggcccgtgta caacaacagg cgatgatcga caaactggat catctgcaac
     2821 gtctcggcat aaacacggtc tttttccagg tcaagccgga cggtaccgcc ctgtggccat
     2881 cgaaaatttt gccgtggtcc gatcttatga ccggtaagat tggtgaaaat ccgggttacg
     2941 atccgctgca atttatgctc gacgaagccc acaagcgtgg gatgaaagta cacgcctggt
     3001 ttaaccccta tcgcgtatcg gttaatacga agcccggtac tatcagggaa ctgaatagca
     3061 ctctgtctca acaaccggcg agcgtctatg tgcaacaccg tgactggatc agaacgtctg
     3121 gcgatcgctt tgtcctcgac ccgggcatcc ctgaggttca ggactggatc acatcaatag
     3181 tcgctgaagt ggtttcccgc tatccggtag atggcgtgca gtttgacgac tatttctata
     3241 ctgaatcacc gggttcacgg ctaaatgata acgaaacgta ccgtaaatac ggaggcgcat
     3301 ttgcgtcaaa agcagactgg cggcgcaaca atactcagca gttaattgca aaggtatcac
     3361 acaccattaa aagcattaag ccgggagtcg aatttggcgt tagcccggca ggcgtgtggc
     3421 gtaaccgatc acacgatccg ctcggttccg atacccgagg cgcggcagcc tatgacgaat
     3481 cctacgctga cacccgtcga tgggtggaac aaggattgct ggattacatt gctccccaaa
     3541 tttactggcc gttctcacgg agtgccgcgc gttatgacgt gttggcaaaa tggtgggcgg
     3601 atgtcgttaa accgaccagg acccgcctgt atatcggtat cgccttctat aaagtgggtg
     3661 aaccttcaaa gatagagcca gactggatga ttaacggcgg cgtaccggaa ctgaaaaagc
     3721 agctcgatct taacgatgcg gtaccagaaa ttagcggcac catcttgttc cgtgaggact
     3781 atctgaataa accgcagact caacaagcgg tcagctatct gcaaagtcgt tggggcagtt
     3841 aaactcccat gcttactttg taacaagccg gtgctttacc caccggcgtg ttacaccttg
     3901 tgaaatatcc cactagtaat catgcttaca taagtcaaat taaccacgca attaaccaca
     3961 cttagttaaa cagctataaa tccctaataa aaatgtatga atatgaaaaa ccatacgata
     4021 ttcgctgtca ccgaaaaata attacgatgg gctatactct tctgaagaac agtagcgtag
     4081 attaaaactg tcctaagtcg cttatttctt ttcagtatat cttcatattt caggagaata
     4141 tggtgcagtt agtcgatctc gcacgttgcg ttttgattcc gtctgatttc ccagccttat
     4201 aagggtgtga attatggaga tgtattttaa aagaatgaaa gatgagtgga ccggacttgt
     4261 cgaacaggca gatcctttca ttcgtgctaa agccgcagaa attgcccttg cgcatgctca
     4321 ttatctgagt atcgagtttt atcgaattgt ccgcatcgac ccgcatgccg aagaattctt
     4381 gagtaatgaa caagttgagc ggcagttgaa aagtgcgatg gaacgctgga ttattaacgt
     4441 gctttctgcc caggttgacg atgtcgaaag gctaatacaa atccagcata ccgtcgcgga
     4501 agtgcatgcc cgcataggaa ttccggtaga aattgtcgag atggggtttc gggtgctgaa
     4561 aaaaatcctc tatccggtaa tcttctcttc ggattattcc gccgcagaaa aacttcaggt
     4621 ctaccatttc tcgattaaca gtattgatat cgctatggaa gtgatgaccc gcgcgtttac
     4681 ctttagtgac agtagtgcct caaaggaaga tgaaaactat cgtatcttct cgttactgga
     4741 aaacgccgaa gaagaaaaag aacggcaaat agcctcaata ctttcatggg aaatagatat
     4801 tatctataaa gtcctgctgg attctgattt aggcagcagt ttgcctttaa gccaggctga
     4861 ttttggcctg tggtttaacc ataaaggtcg acattatttt agcggcattg ccgaagtggg
     4921 ccatatctcc cgtctgattc aggatttcga cggtattttc aatcaaacca tgcgtaacac
     4981 caggaatttg aataacagaa gtttgcgggt gaaatttcta ttacagataa gaaataccgt
     5041 atcgcagatt attaccttgc tgcgtgaatt gtttgaagaa gtatcgcgcc acgaagtcgg
     5101 tatggatgta ctgacgaaat tacttaaccg ccgtttcctg ccgactatct tcaaacgcga
     5161 aattgcccat gccaaccgga ccggtacacc actgtcagtg ctgattattg acgttgataa
     5221 attcaaagag atcaacgata cgtggggcca taacactggc gatgaaattc tgcgtaaagt
     5281 ctctcaggcc ttttatgaca acgtccgcag tagtgattat gtttttcgct acgggggcga
     5341 tgaatttatc attgttttga ctgaagcttc tgaaaacgaa acgttacgta ccgcagaacg
     5401 tattcgcagt cgggtggaga aaaccaaact gaaagccgca aacggcgagg atattgccct
     5461 ctcactttcc atcggtgccg ccatgtttaa tggtcatcct gactatgagc gcctcattca
     5521 aatagccgat gaagctctgt atatcgccaa aagacgaggt agaaaccgtg ttgaactctg
     5581 gaaagccagt ctttagatgc gccaggatgc agaggtaatc atgaagctaa ccgatgcgga
     5641 taatgccgcc gatggcattt ttttccccgc ccttgagcaa aatatgatgg gtgcggtgtt
     5701 aattaacgaa aatgatgaag tgatgttttt caaccccgcc gcagagaagc tctgggggta
     5761 caaacgtgaa gaagtcattg gcaataacat tgatatgctg attccgcggg atttgcgtcc
     5821 tgcgcatcct gaatacattc gtcataaccg tgaaggcggt aaagcgcgtg tcgaggggat
     5881 gagtcgggag ctgcagctgg agaaaaaaga cggcagtaaa atctggaccc gttttgcgct
     5941 atcgaaagtg agcgccgagg ggaaagttta ttacctggcg ctggtacggg atgccagcgt
     6001 agaaatggcg caaaaagaac agactcgaca attgattatt gccgttgacc atctcgaccg
     6061 accggtgatt gtcctcgatc cggaacgcca tattgtgcag tgcaatcgcg catttaccga
     6121 aatgtttggt tactgcatta gcgaagccag cggtatgcag cccgatacac tcctgaacat
     6181 tcctgaattc cctgccgata accgcattcg tttacaacag ttgctatgga aaaccgcccg
     6241 cgatcaggat gaatttctgc tgttgacgcg caccggtgaa aaaatctgga ttaaagcctc
     6301 tatcagcccg gtttatgacg tgctcgcgca tctgcagaac ctggtaatga ccttctcgga
     6361 tatcaccgaa gaacggcaaa tccgccagct tgaaggcaat attctcgccg ccatgtgcag
     6421 cagcccgcca tttcatgaaa tgggggaaat catttgtcgt aacatcgaat ctgttctcaa
     6481 cgaatcgcat gtttcgctgt tcgcactgcg caacgggatg ccgatacact gggcgtcatc
     6541 ttcccacggt gcagaagttc aaaatgcgca aagctggtca gcgaccattc gtcagcgtga
     6601 tggcgcgcct gcggggatcc tgcaaattaa aacctcgtca ggagcagaaa ccagcgcctt
     6661 tatcgaacgc gtggcagata tcagtcagca tatggccgcg ctggcgctgg aacaggaaaa
     6721 aagccgccag catattgaac aactcatcca atttgatccg atgaccggtc tgccaaatcg
     6781 caataacctg cacaattacc tcgatgacct ggtcgacaaa gccgtctctc cggtggtgta
     6841 tctcatcggt gttgaccata ttcaggatgt gattgatagc cttggctatg cgtgggccga
     6901 tcaggcattg ctggaagtgg tcaatcgctt tcgtgaaaaa ctcaaaccgg atcagtatct
     6961 ctgtcgtatc gaaggtacgc agtttgtcct cgtgagcctg gaaaacgacg tcagtaacat
     7021 tacccaaatc gccgatgagc tacggaatgt ggtcagcaag ccgataatga ttgacgataa
     7081 acccttcccg cttaccttga gtattggcat cagctacgac gtgggtaaaa accgcgatta
     7141 cctgctctcc actgctcaca atgcaatgga ttatattcgc aagaatggcg gtaacggctg
     7201 gcagttcttc agcccggcga tgaacgaaat ggtaaaagag cgtttggttt taggcgcagc
     7261 gctgaaagaa gcgattagca ataaccaact gaaacttgtt taccagccgc aaatcttcgc
     7321 agaaacgggt gaactgtacg gcatcgaagc ccttgcgcgc tggcacgatc cccagcatgg
     7381 tcatgtgccc ccttcacggt ttattcctct cgcagaagag attggtgaaa tcgaaaatat
     7441 tgggcgctgg gtcatcgcgg aagcttgccg tcagttagca gaatggcgta gccagaatat
     7501 tcatatcccg gcgctttccg ttaacttgtc ggcactgcac tttcgcagta atcaactgcc
     7561 taatcaggtg tctgatgcaa tgcacgcctg gggtattgac ggccaccagc tgacggtaga
     7621 aatcacggaa agcatgatga tggaacacga taccgaaatc tttaagcgca ttcagatcct
     7681 gcgtgatatg ggcgtgggct tatcggtaga tgattttggt acgggctttt ccggattatc
     7741 ccgcttagtc agtcttccgg taacggaaat caaaattgac aaaagttttg tcgatcgttg
     7801 tctgaccgaa aaacgcatcc ttgccttact tgaagccatt accagcattg ggcaaagcct
     7861 caatttaacc gtcgtggcgg aaggcgtcga aaccaaagag caatttgaga tgctacgcaa
     7921 gatccactgt cgcgttattc agggatattt cttttcccgc cccctacccg ccgaagaaat
     7981 tccaggctgg atgagcagcg tgttaccgct gaaaatctga caaattcctc tcgcccgcac
     8041 tcgcgggttt tcatttaacg tgacactgtc acttaaatac tgtgatttca gctacgattc
     8101 cgggagattc cttccttaac ttcccctctt gcaccaaaac tgcactacgc tgcacaaatg
     8161 ttgatcaaaa gttaaaaaat ataactttaa gccagcattc tggcgcttat cccggcatgg
     8221 catgagattt gcataagcgg aaagcgcagc aatttttgtc ttatacagga accgtttatg
     8281 tcggatacca ccgaactggt tgatttagcc gtaatcttcc ctgatctgga gatcgaattg
     8341 aaatacgcct gcgctgataa catcacaggt aaagctattt atcagcaagc gcgttgtctg
     8401 ctacacaagg atgcgattac cgcgctggcg aaaagtatca gtatcgccca gctgtcaggc
     8461 ttacaactgg tgatttacga tgcgtatcgc ccacaacaag cacaggcgat gttgtggcaa
     8521 gcctgcccag acccgcaata tgttgttgat gtgacggtcg gttctaatca cagccgtggc
     8581 acggcgatcg acctgacgct tcgtgatgag cacgggaaca tcctcgatat gggcgcaggg
     8641 ttcgatgaaa tgcacgagcg ctcccatgcc tatcaccctt ccgtcccgcc cgccgctcag
     8701 cgcaatcggc tgttgctgaa tgcgataatg actggtggcg gttttgtcgg tatctccagc
     8761 gaatggtggc acttcgaatt accccaggca gcgagttacc ctctgctcgc tgatcaattc
     8821 acctgtttta tatcccctgg cacacaacac gtcagttaat caggaaactg tcatgaagag
     8881 atcgataccg tttcgtccca cattgctcgc gctcgtcctt gccacaaatt tcccggttgc
     8941 gcacgccgcc gtaccgaaag atatgctggt gattggtaag gccgccgatc cacaaaccct
     9001 cgacccggcg gtaacaatag ataataacga ctggacggtg acctacccgt cttatcagcg
     9061 gctggttcag tacaaaacgg acggtgataa aggctcaacc gacgttgaag gcgatctggc
     9121 aagtagctgg aaagcgtctg acgatcaaaa agagtggacg ttcaccctga aggacaatgc
     9181 taaatttgcc gatggcacac cggtcactgc cgaagcagta aaactctctt ttgagcggct
     9241 actaaaaatc ggccaggggc cagcagaagc atttcccaaa gatttaaaga ttgatgctcc
     9301 cgacgaacat acggtgaagt ttacccttag ccagccattc gcaccgttcc tctacacgct
     9361 ggcgaatgac ggtgcatcca ttatcaatcc ggcggtgtta aaggaacatg cggcggatga
     9421 tgcccgcggc ttcctcgcgc aaaataccgc cggttccgga ccatttatgc tgaaaagctg
     9481 gcaaaaaggt cagcaattag ttctggtgcc aaatccgcat taccccggca ataaaccgaa
     9541 ctttaagcga gtatcggtaa aaattatcgg tgaaagtgcc tcccgtcgcc tgcagctctc
     9601 ccgtggcgac attgacattg ccgatgcgct gccggtggat caactcaacg ccctgaagca
     9661 ggaaaataaa gtcaatgtgg cagagtatcc gtcactgcgc gttacctatc tgtatctgaa
     9721 taacagcaaa gcgcctctta atcaggcgga tctgcgtcgg gccatttcct ggtctaccga
     9781 ttatcagggc atggttaacg gcattctgag tggtaacgga aaacagatgc gcggcccgat
     9841 tccggaaggc atgtggggct acgatgcgac ggcaatgcaa tacaaccatg acgaaacgaa
     9901 agccaaagcc gaatgggata aagtgacgag caaacccacc agcctgacgt ttctctactc
     9961 cgataacgat ccgaactggg agcctattgc tttggcgaca caatccagtc tcaacaagct
    10021 gggtatcaat gtgaagctgg aaaagctggc gaacgccacc atgcgcgaca gagtgggtaa
    10081 aggtgattac gacattgcga ttggcaactg gagtccggat tttgccgacc cgtatatgtt
    10141 tatgaattac tggtttgagt cagacaaaaa aggtctgccg ggtaaccgct cgttctatga
    10201 aaacagtgag gtcgataagt tactgcgcaa tgcgcttgcg accaccgacc agacgcagcg
    10261 tacccgggac taccagcagg cacagaaaat cgtcattgat gacgctgctt atgtgtacct
    10321 gttccagaaa aactaccaac tggcgatgaa caaagaggtg aaaggctttg tgttcaatcc
    10381 catgctggaa caggtcttca atatcaatac catgagtaaa taacgtgacc ttctggagta
    10441 ttttacgcca acgctgctgg gggctggtgc tcgtggtggc gggcgtctgc gtgattacgt
    10501 ttattatctc gcatctgatc cctggcgatc cggcgcggtt actggggggt gaccgcgcca
    10561 gcgatgctat cgtggaaaat attcgccagc aactgggact ggaccagcca ctgtatgtac
    10621 agttttaccg ctacgtcagc gacctgtttc atggtgacct gggaacatcc attcgtaccg
    10681 ggcgtccggt gctggaagag ttgcgtatat ttttcccggc gacgctggaa ctggcttttt
    10741 gtgccctgct gctggcactc ctgattggca tcccgctggg catactctct gcagtctggc
    10801 gaaatcgctg gctggatcat ctggtgcgaa taatggccat taccggaatc tccacacctg
    10861 cgttctggct tggactgggc gtcattgtgc tgttttatgg tcatctgcaa attcttcccg
    10921 gcggcggaag gcttgatgac tggctggatc caccaacgca cgttaccggc ttttatctgc
    10981 tcgatgcgct gcttgaaggc aacggtgaag tcttcttcaa tgcgttgcaa catctcatct
    11041 taccggcatt aacgctggcg ttcgttcacc tgggaattgt cgctcgccag atccgctcag
    11101 cgatgctgga acaattgagt gaagactaca ttcgtaccgc ccgggccagc ggcttgcccg
    11161 gctggtatat cgttttatgt tatgcgctac ccaatgcgtt gatcccatcg attaccgtat
    11221 tgggtttggc gctgggcgat ttgttgtatg gcgcagtgct caccgaaacc gtttttgcct
    11281 ggcccggaat gggtgcatgg gtagtaacat caatacaggc gctcgacttc ccggcagtga
    11341 tgggctttgc cgtcgtggtt tcatttgctt atgtgctggt caacctggtg gtggatttgc
    11401 tctatttgtg gattgatccg cgtatcggac gtggaggtgg tgaatgatgc taagcgagga
    11461 aacgtccgcc gtacgcccac aaaaacaaac gcgatttaac ggtgcaaaac tggtatggat
    11521 gctgaaaggc agtccgctca ccgtgaccgg cgcagtcatc attgtattaa tgctactgat
    11581 gatgaatttt tcaccgtggc tggcgacgca tgatcccaac gccattgatt taaccgcccg
    11641 ccttttgccg ccttctgcgg cgcactggtt tggcaccgat gaagtgggac gcgatctgtt
    11701 cagccgcgta ctggtcggca gtcagcaatc aattctcgcc ggattagtcg tggttgccat
    11761 tgcgggtatg attggttcgc tactcggatg tctatccggt gtgcttggcg gacgcgcaga
    11821 cgccatcatc atgcgcatca tggacattat gctgtcgatt ccttcgttgg tactgacaat
    11881 ggcactggct gccgctctcg ggccgagttt atttaacgcc atgctggcga ttgctattgt
    11941 gcgaattccc ttttatgtgc gcctggcgcg ggggcaagca ttagttgtac gccagtatac
    12001 ttatgttcag gcggcgaaaa cctttggcgc gtctcgttgg catctgatca actggcatat
    12061 tttacgtaac tccctgccgc cgctgatcgt gcaggcatcg ctggatatcg gtagcgcgat
    12121 tttaatggcc gccacgttgg gatttattgg cctgggtgca caacaaccga gtgctgaatg
    12181 gggcgcgatg gtagcgaatg gtcgcaacta tgtactcgat cagtggtggt attgcgcatt
    12241 tccgggggca gcgattttgc ttaccgccgt cgggtttaat ctctttggtg atggtattcg
    12301 cgatctgctt gacccgaaag caggaggaaa gcagtcatga cccaacccgt tctggacatt
    12361 caacaactgc atttgagttt ccccggtttt aacggcgacg ttcacgcgct caacaatgtg
    12421 tccttgcaga ttaaccgcag tgaaattgtc ggtctggtgg gagaatccgg ctcaggtaaa
    12481 tcagtcaccg caatgctgat tatgcgtctg ctaccgacgg gcagttattg cgtacatcgg
    12541 gggcagattt cactgctggg agaagatgtt cttaacgctc gggaaaaaca gcttcgtcag
    12601 tggcgcggcg cacgagtggc gatgatcttt caggaaccga tgaccgccct caatccgaca
    12661 cgtcgaatag gtcttcagat gatggacgta atccgccatc atcaaccaat aagtcgtcgg
    12721 gaagccagag ctaaagcgat tgccctgctg gaagagatgc aaatcccgga tgccgtggaa
    12781 gttatgtcgc gctatccgtt tgagctttca ggtggtatgc gccagcgggt aatgattgcg
    12841 ctggcattct cctgcgagcc gcaattgatt attgccgacg aaccgactac ggcgctggac
    12901 gtcacgctac agttgcaggt actgcgtttg cttaaacata aagcccgcgc cagtggaact
    12961 gcggtactgt tcatcagcca tgatatggtc gtggtgtcgc aactgtgcga tagagtttac
    13021 gtgatgtatg ccggaagcgt aattgaaagc ggcgtgacgg cagacattat ccatcatccc
    13081 cggcatccgt ataccattgg tttgctgcaa tgcgcaccag aacatggagt accacgccag
    13141 ccattacccg ccattccagg gacggtacca aacctcaccc atttgcctga aggctgcgct
    13201 tttcgcgatc gttgctatgc ggcaggtgca cagtgtgaaa acgtcccggc gctgacagcg
    13261 tgtggtgaca acaaccagcg ctgcgcctgt tggtatccgc agcaggaggt cataagtgtc
    13321 taacacgtta ttaacgttat gcgacgtcca tatcaatttc ccggcccgta aaaactggct
    13381 tggtaaaact acggaacatg ttcatgccat taatggtatt gatttacaga tccgccgtgg
    13441 tgaaacctta gggatcgtcg gcgagtcagg ctgcggcaaa agcaccctcg cacagctttt
    13501 aatgggtatg ctgcaaccga gccacgggca gtacatccgt tctggctcac aacgcattat
    13561 gcagatggtg tttcaggacc cgctctcttc gctcaatccg cgcttaccgg tgtggcgcat
    13621 catcacagaa ccgctctgga tagctaagcg tagtagtgaa caacagcggc gagcgttagc
    13681 agaggagttg gctgtgcagg tgggtattcg tccggagtat ctcgaccgcc tgcctcatgc
    13741 gttctccggc gggcagcggc aacgcatcgc cattgccaga gcactctctt cgcagcctga
    13801 cgtgattgtg cttgatgagc caacctctgc actggatatc tccgtgcagg cgcagatcct
    13861 caatttactg gtaacgctac aggtaaatcg cgggctgacc tatgtgctga tctcacacaa
    13921 tgtctcggtg attcgtcata tgagcgatcg ggtagcggtg atgtatctcg ggcagattgt
    13981 agaactgggc gacgcgcagc aggtgttgac ggcacctgca catccataca cccgattatt
    14041 gctggattcc ctccccgcca ttgataaacc gctggaggaa gaatgggcat tacgtaaaac
    14101 ggatctgccg ggaaaccgca cgttgccgca aggctgtttt ttctacgaac gttgccctct
    14161 ggcaacccac ggatgtgaag tccggcaatc attaacaata agggaggacg gacgtgagat
    14221 ccggtgctgg cgggcgctgt agagatgcta acaacgcatc aggcattcac gcgcctgacg
    14281 cgtcatccgg caatgcttta cgatttcaac tggtaatcaa gcgtaatttc cgctttcagt
    14341 acctgagaaa ccgggcatcc tgcttttgct ttctggatga tgccgtcaaa ggtagaggca
    14401 tcaatacccg gcaccgcaac ttcactcttc agtgcgattt tcgtaatcgc aaaaccggca
    14461 tccactttat ccagcaacac atcggcggtg gtatcaatcg atgttggcgt aaatcccgct
    14521 tcccccagca ttaatgaaag cgccattgag aaacatgcgg catgcgctgc gccaatcagt
    14581 tcttcagggt tagttccttt ttcgccttca aaacgcgtgt taaatccata cggctgttgg
    14641 ttcagcacac cactctcggt ggatactgtt cccttcccgc gtttgatatc gccttcccag
    14701 tgtgcctgac ctttcttatg gattgtcatt gttgctctcc tgtgggctta aaaaggaaat
    14761 tatagactac gtgagaaaca gcacgaggat aagcaggata ttccgaataa aaatccggca
    14821 gaaatattac ttctgcactt aaattaaatt tccacaata
//
LOCUS       DAPHDW010000108        14850 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.109, whole genome shotgun sequence.
ACCESSION   DAPHDW010000108 DAPHDW010000000
VERSION     DAPHDW010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14850)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14850)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14850
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.109"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            79..1368
                     /locus_tag="RH057_003286"
     CDS             79..1368
                     /locus_tag="RH057_003286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HDV3129615.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALANDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPGDRVRVLAEGFPPLENPVVDEREVTTRKSFPTQ
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            1365..2831
                     /gene="hpaE"
                     /locus_tag="RH057_003287"
     CDS             1365..2831
                     /gene="hpaE"
                     /locus_tag="RH057_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3129616.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWANLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALVSPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVGALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            2833..3684
                     /gene="hpaD"
                     /locus_tag="RH057_003288"
     CDS             2833..3684
                     /gene="hpaD"
                     /locus_tag="RH057_003288"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HDV3129617.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     ADEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            3694..4074
                     /locus_tag="RH057_003289"
     CDS             3694..4074
                     /locus_tag="RH057_003289"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HDV3129618.1"
                     /translation="MPHFIVECSDNIREEADLPGLFAKVNPTLAATGIFPLAGIRSRV
                     HWVDTWQMADGQRDYAFVHMTLKIGAGRSLESRQQAGEMLFELIKTHFAALMESRLLA
                     LSFEIEELHPTLNFKQNNVHALFK"
     gene            4185..4988
                     /gene="hpaH"
                     /locus_tag="RH057_003290"
     CDS             4185..4988
                     /gene="hpaH"
                     /locus_tag="RH057_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HDV3129619.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAEGRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLEAGQIILGGSFTRPVPARKGDTFHVDYGNMGSISCRFV"
     gene            4999..5787
                     /gene="hpaI"
                     /locus_tag="RH057_003291"
     CDS             4999..5787
                     /gene="hpaI"
                     /locus_tag="RH057_003291"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HDV3129620.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGAGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     EAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGAQATTVKPGVY"
     gene            5962..7338
                     /gene="hpaX"
                     /locus_tag="RH057_003292"
     CDS             5962..7338
                     /gene="hpaX"
                     /locus_tag="RH057_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HDV3129621.1"
                     /translation="MSDTSPAIPESIDPANQHKALTAGQQAVIKKLFRRLIVFLFVLF
                     IFSFLDRINIGFAGLTMGRDLGLSATMFGLATTLFYAAYVIFGIPSNIMLSIVGARRW
                     IATIMVLWGIASTATMFATGPTSLYVLRILVGITEAGFLPGILLYLTFWFPAYFRARA
                     NALFMVAMPVTTALGSIVSGYILSLDGVMALKGWQWLFLLEGFPSVLLGVMVWFWLDD
                     SPDKAKWLTKEDKKCLQEMMDNDRLTLVQPEGAISHHAMQQRSMWREIFTPVVMMYTL
                     AYFCLTNTLSAISIWTPQILQSFNQGSSNITIGLLAAVPQICTILGMVYWSRHSDRRQ
                     ERRHHTALPYLFAAAGWLLASATDHNMIQMLGIIMASTGSFSAMAIFWTTPDQSISLR
                     ARAIGIAVINATGNIGSALSPFMIGWLKDLTGSFNSGLWFVAALLVIGAGIIWAIPMQ
                     SSRPRATP"
     gene            7348..8238
                     /gene="hpaA"
                     /locus_tag="RH057_003293"
     CDS             7348..8238
                     /gene="hpaA"
                     /locus_tag="RH057_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HDV3129622.1"
                     /translation="MCDRQIANIDISKEYDESLGTDDVHYQSFARMAAFFGRHMLPHR
                     HEQYFQMHFLNSGQIELQLDDHRYSVEAPLFVLTPPSVPHAFITESDADGHVLTVRED
                     LIWPLLEVLYPGTRETFGLPGICLSLADKPDELAALEHYWQLIERESVEQLPGREHTL
                     TLLAQAVFTLLLRNAKLDDHAASGMRGELKLFQRFNMLIESHFHQHWTVPDYANELHI
                     TESRLTDICRRFANRPPKRLIFDRQLREAKRLLLFSDNAVNNIAWQLGFKDPAYFARF
                     FNRLVGCSPSAYRAKKVPVT"
     gene            8489..10051
                     /gene="hpaB"
                     /locus_tag="RH057_003294"
     CDS             8489..10051
                     /gene="hpaB"
                     /locus_tag="RH057_003294"
                     /EC_number="1.14.14.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
                     /protein_id="HDV3129623.1"
                     /translation="MKPEDFRASTQRPFTGEEYLKSLQDGREIYIYGERVKDVTTHPA
                     FRNAAASVAQLYDALHKPEMQDSLCWNTDTGSGGYTHKFFRVAKSADDLRQQRDAIAE
                     WSRLSYGWMGRTPDYKAAFGCALGANPGFYGQFEQNARNWYTRIQETGLYFNHAIVNP
                     PIDRHLPTDKVKDVYIKLEKETDAGIIVSGAKVVATNSALTHYNMIGFGSAQVMGENP
                     DFALMFVAPMDADGVKLISRASYEMVAGATGSPYDYPLSSRFDENDAILVMDNVLIPW
                     ENVLIYRDFDRCRRWTMEGGFARMYPLQACVRLAVKLDFITALLKKSLECTGTLEFRG
                     VQADLGEVVAWRNTFWALSDSMCSEATPWVNGAYLPDHAALQTYRVLAPMAYAKIKNI
                     IERNVTSGLIYLPSSARDLNNPQIDQYLAKYVRGSNGMDHVQRIKILKLMWDAIGSEF
                     GGRHELYEINYSGSQDEIRLQCLRQAQSSGNMDKMMAMVDRCLSEYDQNGWTVPHLHN
                     NDDINMLDKLLK"
     gene            10069..10581
                     /locus_tag="RH057_003295"
     CDS             10069..10581
                     /locus_tag="RH057_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HDV3129624.1"
                     /translation="MQLDEQRLRFRDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            10974..13124
                     /gene="btsT"
                     /locus_tag="RH057_003296"
     CDS             10974..13124
                     /gene="btsT"
                     /locus_tag="RH057_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HDV3129625.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLKVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            13255..13458
                     /locus_tag="RH057_003297"
     CDS             13255..13458
                     /locus_tag="RH057_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HDV3129626.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            13469..14425
                     /gene="yjiA"
                     /locus_tag="RH057_003298"
     CDS             13469..14425
                     /gene="yjiA"
                     /locus_tag="RH057_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HDV3129627.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQRYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDETPHSTMVFIGIQLPEDEIRAAFAGLRK"
     gene            complement(14512..14703)
                     /locus_tag="RH057_003299"
     CDS             complement(14512..14703)
                     /locus_tag="RH057_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HDV3129628.1"
                     /translation="MSMVKHKRGTLYTLDVQQEAELMALVNKSDEDIDYSDIPASGSE
                     AWSDAKRGNFLPDPGIHAQ"
BASE COUNT         3415 a         4085 c         4083 g         3267 t
ORIGIN      
        1 ttgccaaact acgcaatcgg gaataaaagt aatcattaac atattaatga ttaaaatgat
       61 cgaatccgag gagtggtaat gaaaggcact atcttcgccg tagcgttgaa ccatcgcagc
      121 caacttgatg catggcagga agcgttccag caatccccct acaaagcccc gcctaaaact
      181 gcggtctggt ttattaaacc gcgcaatacg gtgattggtt gcggtgaacc gattcccttt
      241 ccacagggtg aaaaggtact gagcggtgcg actgttgcgc tgattgtggg aaaaacggcg
      301 acgaaagtac gtgaagaaga tgcggcagag tacatcgccg gatatgcgct ggctaacgac
      361 gtcagcctgc cggaagagag cttttaccgc ccggcaatca aagcaaaatg tcgtgatgga
      421 ttctgcccca ttggcgaaac cgtggctctc agcaatgtcg ataatctgac catctatacc
      481 gagatcaacg ggcgtcctgc cgatcactgg aataccgccg atttacaacg taacgccgcg
      541 cagttgctga gcgccctgag cgaatttgcc acgctgaatc caggcgacgc cattctgctc
      601 ggcacgccac aggcgcgcgt ggaaatacag ccaggcgatc gcgttcgtgt tctcgcagaa
      661 ggtttcccgc cgctggaaaa tccggtagtg gacgaacgtg aagtgaccac gcgcaagagc
      721 ttcccaacgc agccacaccc gcacggcacg ctgtttgccc tcggcctgaa ctacgccgac
      781 cacgccagcg aactggaatt taagccaccg gaagaaccgc tggtgttcct gaaagcgcca
      841 aataccctca ctggcgataa ccagacctcc gtgcgtccaa acaatattga atacatgcac
      901 tatgaagcgg agctggtggt agttattggc aaacaggcgc gtaacgtcag cgaagccgat
      961 gccatggatt atgtcgcggg ctacaccgtg tgtaacgact acgccattcg cgactatctg
     1021 gaaaactact accgccctaa cctgcgggtc aaaagccgcg acggactgac gccgatgctt
     1081 tcaaccatcg tgccgaaaga ggcgatcccg gacccgcata atctgaccct tcgcaccttc
     1141 gtcaacggcg agttacgcca gcaaggcacc accgccgatc tgatcttcag cgtgcccttc
     1201 ctgatcgcct acttaagcga atttatgacc ctgaatccgg gcgacatgat cgccaccggc
     1261 acaccaaaag gcttatctga cgtggtgcct ggcgatgaag tagtggtgga agtagaaggc
     1321 gtgggccgcc tggtgaaccg aattgtgagt gaggaaacag cgaaatgaaa aaagtaaatc
     1381 attggatcaa cggcaaaaat gttgcaggta acgactactt ccagaccacc aatccggcaa
     1441 cgggtgaagt gctggcggat gtggcctctg gcggtgaagc ggagatcaat caggcggtag
     1501 cggcagcgaa agaggcgttc ccgaaatggg ccaatctgcc aatgaaagag cgtgcgcgcc
     1561 tgatgcgccg tctgggcgat ctgatcgacc agaacgtgcc agagatcgcc gcgatggaaa
     1621 ccgcggacac gggcctgcca atccatcaga ccaaaaatgt gttgatccca cgcgcttccc
     1681 acaactttga atttttcgcg gaagtctgcc agcagatgaa cggcaagacc tatccggttg
     1741 acgacaagat gctcaactac acgctggtgc agccggtggg cgtttgtgcg ctggtatcgc
     1801 cgtggaacgt accgtttatg accgccacat ggaaggtcgc gccgtgtctg gcgctgggca
     1861 ataccgcggt actgaaaatg tcggaactct ccccgctgac cgctgaccgc ctgggtgagc
     1921 tggcgctgga agccggtatt ccggcaggcg tgctgaacgt ggtacagggc tacggcgcaa
     1981 ccgcagggga tgcgctggtt cgtcatcatg acgtacgtgc cgtgtcgttc accggcggta
     2041 cggccaccgg gcgcaacatc atgaaaaacg ccgggctgaa aaaatactcc atggaactgg
     2101 gcggtaaatc gccggtgctg atttttgaag atgccgatat tgaacgcgcg ctggacgccg
     2161 ccctgttcac catcttctcg atcaacggcg aacgctgcac cgccggttcg cgcatcttta
     2221 ttcagcaaag catctacccg gaattcgtta aacgctttgc cgaacgcgcc aaccgtctgc
     2281 gcgtgggcga tccgaacgat ccgaataccc aggttggcgc gcttatcagc cagcagcact
     2341 gggaaaaagt ctccggctat atccgtctcg gcattgaaga aggcgcaacc ctgctggcgg
     2401 gcggcccgga taaaccgtcc gacctgcctg cgcacctgaa aggcggcaac ttcctgcgcc
     2461 caaccgtgct ggcagacgtt gataaccgta tgcgcgtcgc ccaggaagag attttcgggc
     2521 cggtcgcctg cctgctgccg tttaaagacg aagctgaagg cttacgcctg gcaaacgacg
     2581 tggagtacgg cctcgcgtcg tacatctgga ctcaggatgt cagcaaagtg ctgcgtctgg
     2641 cgcgtggcat tgaagctggc atggtgttcg tcaacaccca gaacgtgcgt gatttgcgcc
     2701 agccgtttgg cggcgtaaaa gcctccggca ccgggcgtga aggcggtgag tacagcttcg
     2761 aagtgttcgc ggaaatgaag aacgtctgca tttccatggg cgaccatcca attccgaaat
     2821 ggggagtctg atatgggtaa gttagcgtta gccgccaaga tcacgcacgt accgtcgatg
     2881 tatctctctg aactgccagg gaaaaaccac ggttgccgcc agggcgcgat cgacgggcat
     2941 aaagagatca gcaagcgttg ccgggaaatg ggcgtcgata ccattatcgt tttcgatacc
     3001 cactggctgg tcaacagtgc ttatcacatc aactgtgcag accattttga aggcgtctac
     3061 accagcaacg agctgccgca ttttatccgc gatatgacct acaactacga gggcaacccg
     3121 gagttggggc agcttattgc cgatgaagcc ttaaagctcg gcgtgcgggc aaaagcgcac
     3181 aacattccca gcctgaaact ggaatacggc acgctggtgc caatgcgcta catgaatgaa
     3241 gacaagcact tcaaagtggt ttccatttca gctttctgca ctgttcacga ttttgccgac
     3301 agccgcaagc tgggcgaggc gattctgaaa gcgatcgaac agtacgacgg caccgtggcg
     3361 gtccttgcca gcggttcgtt atcgcaccgc tttattgacg atcagcgagc ggaagaaggg
     3421 atgaacagct acacccgcga gttcgaccgc cagatggacg agcgtgtggt gaagctgtgg
     3481 cgcgaaggcc agttcaaaga gttctgcaat atgctgccgg agtacgccga ctactgctac
     3541 ggcgaaggca atatgcacga cacggtgatg ctgctgggga tgctcggctg ggataaatac
     3601 gacggcaagg tggagtttat caccgagcta ttcccaagct ctggcaccgg tcaggttaac
     3661 gctgttttcc cgcttcccgc gtaaggacgt tttatgccgc actttatcgt tgaatgcagt
     3721 gacaacatcc gcgaagaagc cgacctgccg gggttgttcg ccaaagtgaa tccgacgctg
     3781 gcagccacgg gtatttttcc gctggcgggt attcgcagcc gcgtgcattg ggtcgatacc
     3841 tggcagatgg ccgacgggca gcgtgattat gccttcgtgc atatgacgtt gaaaatcggc
     3901 gcaggtcgca gcctggaaag ccgtcagcag gcgggtgaaa tgctgtttga actgattaaa
     3961 acgcacttcg ccgccctgat ggagagccgc ctgctggcgt tgtcgtttga gattgaagag
     4021 ctgcatccga cgctgaattt taaacaaaac aacgtgcacg cattgtttaa gtagcgcgca
     4081 gattgcccgg tggcgctgcg cttaccgggc ctacaaaacc ccaaaccgta caccgtaggc
     4141 cggataaggc gcagccgcat ccggcaatgc cacaggatat cgctatgttc gataaacata
     4201 cccacaccct gatcgcccag cgtctggatc aggcagaaaa acagcgcgaa cagatccgcg
     4261 cgatctcact ggattacccg gagatcacca tcgaagacgc ttacgcggtg cagcgcgaat
     4321 gggttcgcct gaaaatcgcc gaaggtcgca cgctgaaagg ccacaaaatc ggcctgacct
     4381 cgaaagcgat gcaggcaagc tcgcagatca gcgaaccgga ttacggcgcg ctgctggatg
     4441 acatgttctt ccacgatggc agcgatatcc cgaccgatcg ctttatcgtg ccgcgcattg
     4501 aagtggagct ggctttcgtg ctggcaaaac cgctgcgtgg gccaaactgc acgctgttcg
     4561 acgtctacaa cgccacggac tacgtgatcc cggcgctgga gctgatcgac gctcgctgcc
     4621 acaacatcga tccggaaacc cagcgcccgc gcaaagtgtt cgacaccatt tctgataacg
     4681 ccgccaacgc cggggtgatc ctcggtggtc gtcccattaa gcccgatgag ttggatctgc
     4741 gttggatctc cgccctgatg tatcgcaatg gcgtgattga agaaaccggc gtcgccgctg
     4801 gtgtgctgaa tcatccggca aacggcgtgg cctggctggc gaacaaactc gcaccgtatg
     4861 acgtacaact ggaagccggg caaatcattc tcggcggttc gttcacccgc ccggttccgg
     4921 cgcgtaaggg cgacaccttc cacgtcgatt acggcaacat gggctccatt agctgccgct
     4981 ttgtttaagg agagaacgat ggaaaacagt tttaaagcgg cgctgaaagc aggccgcccg
     5041 cagatcggat tatggctggg gctgagtagc agctacagcg cagagttact ggccggagca
     5101 ggattcgact ggttattgat cgacggtgag cacgcgccga ataacgtgca aaccgtgctc
     5161 acccagctac aggcgattgc gccctacccc agccagccgg tggtacgtcc gtcgtggaac
     5221 gatccggtgc aaatcaaaca actgctggac gtcggcacac aaaccttgct ggtgccgatg
     5281 gtacaaaacg ctgacgaagc ccgtgaagcg gtacgcgcca cccgttatcc ccccgccggt
     5341 attcgcggtg tgggcagtgc gctggcccga gcctcgcgct ggaatcgtat tcctgattac
     5401 ctgcaaaaag ccaacgatca aatgtgcgtg ctggtgcaga tcgaaacgcg tgaggcaatg
     5461 aagaacttac cgcagattct ggacgtggaa ggggtcgacg gcgtgtttat cggcccggcg
     5521 gatctgagcg ccgatatggg ttatgccggt aatccacagc acccggaagt acaggccgcg
     5581 attgagcagg cgatcgtgca gatccgcgaa gcgggcaaag cgccggggat cctgatcgcc
     5641 aatgagcaac tggcaaaacg ctatctggaa ctgggcgcgc tgtttgtcgc cgtcggcgtt
     5701 gacaccaccc tgctcgcccg cgccgctgaa gcgctggcag cacggtttgg cgcacaggcc
     5761 acgaccgtta agcccggcgt gtattaatgc cggatgcgct gcacttatcc ggcctacact
     5821 cgcaccaaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaaaa gccgtaccaa
     5881 atcgcggacc ggataaggcg tttgtgcgca tccgccataa accttgtcgt accctacaaa
     5941 aatcccatta gaggaagaaa aatgagcgac acctcacctg ccataccgga gagtatcgat
     6001 ccggcgaatc agcataaagc gctgactgcc ggacaacagg cggttattaa gaagctgttt
     6061 cgccgcctga tcgtctttct gttcgtgctg tttatcttct cgttccttga tcgcatcaac
     6121 atcggctttg ccggactcac gatgggacgc gacctcggtc tgagcgccac catgtttggc
     6181 ctcgctacca ccctgttcta tgccgcttat gtcatcttcg gcattcccag caacattatg
     6241 ctgagtattg tcggtgcgcg gcgctggatc gccaccatca tggtgctttg gggcatcgcc
     6301 tctactgcca ccatgtttgc cactggcccc accagcttgt acgtactgcg tatactggtt
     6361 ggcattaccg aagccggctt tctgcccggc attctgctgt atttaacctt ctggtttccg
     6421 gcctatttcc gcgcccgtgc caacgccttg tttatggtag caatgccggt aacgacagcg
     6481 ttgggctcga tcgtttccgg ctacattttg tcgctggatg gcgtcatggc attaaaaggc
     6541 tggcagtggc tgtttttgct ggaaggcttc ccgtcggtat tactcggcgt catggtgtgg
     6601 ttctggcttg atgactcacc ggacaaagct aagtggctga cgaaagaaga caaaaaatgc
     6661 ctgcaagaga tgatggataa cgatcgtctg acgctggttc agccagaggg agccatcagc
     6721 caccacgcca tgcaacaacg cagtatgtgg cgggagatct tcactccggt ggtgatgatg
     6781 tataccctgg cgtatttctg cctgaccaac acacttagtg cgatcagcat ctggacaccg
     6841 cagatcctgc aaagttttaa tcagggcagc agtaatatca ccatcggcct gctggccgcc
     6901 gtaccgcaga tttgtaccat tctcgggatg gtctactgga gccgtcactc agatcgccgc
     6961 caggaacgaa ggcatcacac cgcccttcct tatttgttcg ctgccgcagg ttggttactg
     7021 gcttcggcaa ctgatcacaa catgatccag atgctgggga tcattatggc ttcgaccgga
     7081 tcattcagcg caatggcgat tttctggaca acaccggatc agtccatcag cctgcgggca
     7141 cgagcgatcg gtattgcggt gatcaacgcc actggcaaca ttggttcagc gttaagtccg
     7201 tttatgatcg gctggttgaa agatttgacc ggcagcttta acagtggatt gtggtttgtt
     7261 gccgcgctgc tggtgattgg tgcggggatt atctgggcga ttccaatgca gtcctcccgt
     7321 ccgcgagcga ccccgtaagg aacgacgatg tgtgaccgtc agattgccaa tattgatatc
     7381 agcaaagagt acgatgaaag cctgggcacg gacgatgtgc attatcagtc cttcgcccgc
     7441 atggcggcct tttttggccg ccatatgctg ccacatcgcc acgaacagta ctttcagatg
     7501 catttcctca atagcggaca gattgagcta caacttgacg atcatcgcta ctcggtggaa
     7561 gcgcccctgt ttgtcctgac gccgccgtca gtacctcatg cgtttattac ggagtctgac
     7621 gccgacggtc atgtgttgac ggtacgggaa gatctgatct ggcccctgct ggaagttctt
     7681 tatccgggca ctagggaaac cttcggcctg ccggggattt gcctgtcact ggcagataaa
     7741 cccgacgaac tggcggcgct ggaacactat tggcaactga tagagcggga atcggtggaa
     7801 caactgcctg gacgggaaca caccctgacg ttactggcac aggcagtgtt caccctactg
     7861 ctgcgtaacg caaaactcga cgaccatgcc gccagcggga tgcgcggaga attaaaactg
     7921 ttccagcgtt ttaatatgct gattgaaagc cattttcatc agcactggac agtaccggat
     7981 tacgctaacg aactgcatat caccgaatca cgactcacgg acatctgccg tcgctttgcc
     8041 aaccgtccgc caaaacggtt gattttcgac aggcagctgc gagaagccaa gcggctgctg
     8101 ctgttttctg ataacgccgt gaacaatatt gcctggcaac tcggttttaa ggatccggct
     8161 tattttgcgc gcttttttaa tcgcttagtc ggttgctcgc ccagtgctta tcgcgccaaa
     8221 aaagtacctg tgacgtgaaa atcccttagc cttaacggga aaccaggcac cacctgctat
     8281 tcccctttct ccgcctggag caaggggatt tagtaacgtc aaaaatcgct aaaagcgacc
     8341 tcgatcacaa aaccgtaccc gacctgaaaa gtaccagcca ttgtcccaaa agtgtcttcc
     8401 cgtaatcgcc cctgcaagct tcaattgaat aacaaaaaca acacacaaaa ttaacaatcg
     8461 tattccgatt aatactgtag aggtcgacat gaaaccagaa gatttccgcg ccagtaccca
     8521 acgtccgttc accggggaag agtatctgaa aagcctgcag gatggtcgcg agatctatat
     8581 ctatggcgag cgagtgaaag acgtcaccac tcatccggca tttcgtaatg cggcagcgtc
     8641 tgttgcccag ctgtacgacg cactgcacaa accggagatg caggactctc tgtgctggaa
     8701 caccgacacc ggcagcggcg gctataccca taaattcttc cgcgtggcga aaagtgccga
     8761 cgacctgcgc cagcaacgcg acgccatcgc tgagtggtca cgcctgagct atggctggat
     8821 gggccgtacc ccagactaca aagctgcttt cggttgcgca ctgggcgcga atccgggctt
     8881 ttacggtcag ttcgagcaga acgcccgtaa ctggtacacc cgtattcagg aaactggcct
     8941 ctactttaac cacgcgattg ttaacccacc gatcgatcgt catttgccga ccgataaagt
     9001 aaaagacgtt tacatcaagc tggaaaaaga gactgacgcc gggattatcg tcagcggtgc
     9061 gaaagtggtt gccaccaact cggcgctgac tcactacaac atgattggct tcggctcggc
     9121 acaagtaatg ggcgaaaacc cggacttcgc actgatgttc gttgcgccaa tggatgccga
     9181 tggcgtgaaa ttaatctccc gcgcctctta tgagatggtc gcgggtgcta ccggctcacc
     9241 gtatgactac ccgctctcca gccgcttcga tgagaacgat gcgattctgg tgatggataa
     9301 cgtgctgatc ccatgggaaa acgtgctgat ctaccgcgat tttgatcgct gccgtcgctg
     9361 gacgatggaa ggcggtttcg cccgtatgta tccgctgcaa gcctgtgtgc gcctggcagt
     9421 gaaactcgac ttcattacgg cactgctgaa aaaatcactc gaatgtaccg gcaccctgga
     9481 gttccgtggt gtgcaagccg atctcggtga agtggtggcg tggcgcaaca ccttctgggc
     9541 attgagtgac tcgatgtgtt ctgaagcgac gccgtgggtc aacggggctt atttaccgga
     9601 tcatgccgca ctgcaaacct atcgcgtact ggcaccaatg gcctacgcga agatcaaaaa
     9661 cattatcgaa cgcaacgtta ccagtggcct gatctatctc ccttccagtg cccgtgacct
     9721 gaacaatccg cagatcgacc agtatctggc gaagtatgtg cgcggttcga acggtatgga
     9781 tcacgtccag cgcatcaaga tcctcaaact gatgtgggat gccattggca gcgagtttgg
     9841 tggtcgtcac gaactgtatg aaatcaacta ctccggtagc caggatgaga ttcgcctgca
     9901 gtgtctgcgc caggcacaaa gctccggcaa tatggacaag atgatggcga tggttgatcg
     9961 ctgcctgtcg gaatacgacc agaacggctg gactgtgcct cacctgcaca acaacgacga
    10021 tatcaacatg ctggataagc tgctgaaata acgcagcagg aggttaagat gcaattagat
    10081 gaacaacgcc tgcgctttcg tgacgcgatg gccagcctgt cggcagcggt aaatattatc
    10141 accaccgagg gcgacgccgg acaatgcggg attacggcaa cggccgtctg ctcggtcacg
    10201 gatacaccac catcgctgat ggtgtgcatt aacgccaaca gtgcgatgaa cccggttttt
    10261 cagggcaacg gcaagttgtg cgtcaacgtc ctcaaccatg agcaggaact gatggcacgc
    10321 cacttcgcgg gcatgacggg catggcgatg gaagagcgtt ttagcctctc atgctggcaa
    10381 aaaggtccgc tggcgcagcc ggtgctaaaa ggttcgctgg ccagtcttga aggtgagatc
    10441 cgcgatgtgc aggcaattgg cacacatctg gtgtatctgg tggagattaa aaacatcatc
    10501 ctcagtgcag aaggtcacgg acttatctac tttaaacgcc gtttccatcc ggtgatgctg
    10561 gaaatggaag ctgcgattta agctccagcc ccttcacgca ttcgtcgaag gggcattttt
    10621 attgtgatct caatcacata tccacccgat gaataattct tgcccaaccg ctcaccgcca
    10681 ttcctgaccg cttacatccc taaaataacc actcagttat ttaccttact ttacgcgcgc
    10741 gtatctctgg caacatcact gcaggatagc ggtcaattta cctcctcaaa cgcaacgcaa
    10801 acctagaacg gcttcggcct atgattaaca aattactcta tggattttgg tttgcaggaa
    10861 ggcagcaagt gagtgaatcc ccgggagctt gcaacagtaa gtgacagggg tgaacgaacg
    10921 cagctgccgc acctgtaagc caaaagacga cgagtaaaat gccaggtttt actatggata
    10981 cgaaaaaact attcaagcac ataccctggg tgattctcgg aatcatcggt gcattctgcc
    11041 tcgcggtagt tgcattacgt cggggggagc acgtcagcgc cctgtggatc gtggtcgcct
    11101 ctgtgtcggt gtatctggtg gcgtatcgct actacagttt gtatatcgcc cagaaggtga
    11161 tgaaactcga ccccacgcgc gcgacgcctg cggttattaa caacgacggt ctgaactacg
    11221 ttccgaccaa ccgttacgtg ttgtttggtc accacttcgc cgctatcgcc ggtgctggtc
    11281 cgctggttgg tccggttctc gccgcgcaga tgggctacct acctggcacg ctgtggctgc
    11341 tggcgggggt agtactggcc ggtgcggtac aggactttat ggtgctgttt atctcctctc
    11401 gccgtaacgg cgcatctctc ggtgagatga tcaaagaaga gatgggacca gtaccgggga
    11461 ctatcgcgct gtttggctgt ttcttaatca tgatcatcat ccttgccgtc ctggcgctga
    11521 ttgtggttaa agccctggcc gaaagcccgt ggggtgtttt caccgtttgc tcaaccgtac
    11581 cgattgcgct gtttatgggt atctacatgc gctttatccg tccggggcgt gtgggtgaag
    11641 tctctgtcat tggtatcgtg ctgctggttg cctctatcta cttcggtggc gtgattgctc
    11701 acgatccgta ctggggtccg gcactgacct ttaaagacac caccattacc ttcgcgctga
    11761 ttggctatgc gtttgtttcc gcactgctgc cggtgtggct gatcctcgca ccgcgcgact
    11821 atctggcaac cttcctgaaa atcggcgtta tcgtcggcct ggcgctgggt attgtggtgc
    11881 tgaacccgga actgaaaatg cctgcgatga cccagtacat tgacggtact ggcccgctgt
    11941 ggaaaggcgc tctgttcccg ttcctgttca tcaccatcgc ctgtggtgcg gtatctggct
    12001 tccacgcgct gatctcttcc ggtacgactc cgaaactgct ggctaacgaa accgacgcgc
    12061 gtttcattgg ctacggcgca atgctgatgg agtccttcgt ggcgattatg gcgctggtcg
    12121 ctgcgtccat catcgaaccg ggtctttact tcgcgatgaa caccccgcct gctggccttg
    12181 gcattaccat gcctaacctg catgaaatgg gtggcgagaa tgcgccgatc atcatggcgc
    12241 agctgaaaga cgttaccgca cacgcggcgg caaccgtcag ctcctggggc ttcgtgattt
    12301 cgccagagca gatcctgcaa accgcgaaag acattggtga gccttctgtc ctgaaccgtg
    12361 caggtggtgc gccaacgctg gcggtaggta tcgctcacgt gttccacaaa gtgctgccga
    12421 tggctgacat gggcttctgg tatcacttcg gtattctgtt cgaagccctg ttcatcctga
    12481 ccgcgctgga tgcgggtact cgttctggcc gctttatgct gcaagacctg ctgggtaact
    12541 tcatcccgtt cctgaaaaaa accgactctc tggttgccgg tatcatcggt actgcgggct
    12601 gtgtgggtct gtggggctac ctgctgtatc agggcgtggt cgatccgctg ggcggcgtta
    12661 agagcctgtg gccgctgttc ggtatttcca accagatgct ggcagccgta gcgctggtac
    12721 tgggcaccgt tgtgctgatt aagatgaagc gcacccaata catctgggta actgttgttc
    12781 cggctgtatg gctgcttatc tgcaccacct gggcgctggg tctgaaactg ttcagcacca
    12841 acccgcagat ggaaggcttc ttctacatgg caagccagta caaagagaag attgctaacg
    12901 gtactgacct gacggcgcag cagattgcca acatgaacca catcgttgtg aacaactaca
    12961 ccaacgcagg cctgagtatt ctgttcctga ttgtggtgta cagcatcatc ttctacggtt
    13021 tcaaaacctg gctgaaggtg cgtaacagcg acaaacgtac tgacaaagaa actccgtacg
    13081 ttccaatccc ggaaggcggc gtgaagatct cttcgcacca ctaaccggtt gccggatgcg
    13141 ctgcgcgtat ccggcctaca aagtgtctat cgtaggccgg ataaagcgtt tacgccgcat
    13201 ccggcaaatc cctagcccgg accttccggg ctttacgcat atctggaagg caaaatgttt
    13261 ggtaacttag gacaggcaaa aaaatatctc ggccaggcgg cgaagatgtt gattggcatt
    13321 ccagactacg acaactacgt cgagcatatg aagaccaacc atcccgacaa gccgtacatg
    13381 agctatgaag aattcttccg cgaacgccag aacgcgcgct acggcggcga tggtaaaggc
    13441 ggtatgcgct gttgttaatg gagagagcat gaacccgatt gcagttaccc tactcaccgg
    13501 ttttttaggc gcgggcaaaa ccaccctgct gcgccatatt cttaacgaac aacacggcta
    13561 caagattgcc gtgattgaaa acgaattcgg cgaagtctcc gttgatgatc aactgattgg
    13621 cgaccgcgcc acgcagatca aaacgctgac caacggctgc atctgctgtt cgcgctccaa
    13681 cgagctggaa gacgcactac tcgacctgct ggataatctc gacaagggca atattcagtt
    13741 cgaccgtctg gtcattgaat gcaccggcat ggccgatccc ggcccgatta ttcagacctt
    13801 tttctcccat gagattttat gccagcgcta cctgctggac ggggtgattg cgctggtgga
    13861 tgcggtacat gccgatgagc agatgaacca gttcaccatc gcccagtccc aggttggcta
    13921 cgccgaccgt attctgctga ctaaaaccga cgtcgcaggc gaagcagaga aactgcgtga
    13981 acgcctggcg cgcatcaacg cccgtgcacc ggtctacacc gtcacccacg gcgatatcga
    14041 cctgggcctg ctattcaaca ccaacggttt tatgctggaa gagaacgtgg tcagcaccaa
    14101 gccacgtttc cactttatcg cggacaaaca aaacgatatt tcgtcgattg tggtggaact
    14161 ggattacccg gtagatatca gcgaagtttc ccgggtgatg gaaaacctgc tgctggaatc
    14221 ggcggataaa ctgctgcgtt acaaagggat gctgtggatt gacggcgaac ctaaccgcct
    14281 gctgttccag ggcgtccagc gcctctacag cgccgactgg gaccgcccgt ggggcgatga
    14341 aacaccgcat agcacgatgg tgtttatcgg gattcagttg ccggaggatg agattcgggc
    14401 ggcgtttgcg gggttgagga agtaatcgtg aaaggttgag tgtaaagcag ctcaatgccc
    14461 catttagcag tgagggggga aacagaacga cgtgctcccc tccgctaagt tttattgcgc
    14521 gtgaatacct ggatctggca aaaaattgcc gcgtttggcg tcagaccatg cttcgctacc
    14581 agaagcagga atgtcgctgt aatcaatgtc ttcgtcggat ttattaacca aagccatgag
    14641 ttcagcttct tgctgcacat caagtgtgta gagtgtgccc cgtttatgtt taaccatgct
    14701 catttatgac cagaaccttt acgatttaat tataaagatg acttatttca gccatccagg
    14761 cacgttatat tctgagacat tctcaatgga atatgcatct ctcctgtcag aacctttctt
    14821 acacttcaac tcattgaacc cccatttacc
//
LOCUS       DAPHDW010000109        14823 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.110, whole genome shotgun sequence.
ACCESSION   DAPHDW010000109 DAPHDW010000000
VERSION     DAPHDW010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.110"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(73..609)
                     /gene="rcdA"
                     /locus_tag="RH057_003300"
     CDS             complement(73..609)
                     /gene="rcdA"
                     /locus_tag="RH057_003300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HDV3129629.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            693..1901
                     /locus_tag="RH057_003301"
     CDS             693..1901
                     /locus_tag="RH057_003301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3129630.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     RYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            1901..2716
                     /gene="ybjI"
                     /locus_tag="RH057_003302"
     CDS             1901..2716
                     /gene="ybjI"
                     /locus_tag="RH057_003302"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HDV3129631.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVVSEGKDVFNGELSKDAFTTVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGNAVVAAAKYRAGSNNREGVLDVIDKVLKHEAPFDQ"
     gene            2802..3086
                     /locus_tag="RH057_003303"
     CDS             2802..3086
                     /locus_tag="RH057_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129632.1"
                     /translation="MFMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3127..4359)
                     /gene="mdfA"
                     /locus_tag="RH057_003304"
     CDS             complement(3127..4359)
                     /gene="mdfA"
                     /locus_tag="RH057_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HDV3129633.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIITCLAILLA
                     QNIEQFTLLRFLQGISLCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQQAMPETATRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLA
                     RLTSRRTVRSLIIMGGWPIIIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGL
                     VRLTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWL
                     SLMVIFLKDKQMGNSHEG"
     gene            4644..5240
                     /gene="ybjG"
                     /locus_tag="RH057_003305"
     CDS             4644..5240
                     /gene="ybjG"
                     /locus_tag="RH057_003305"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HDV3129634.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRFCFALPIRKGWVRD"
     gene            5298..6056
                     /gene="deoR"
                     /locus_tag="RH057_003306"
     CDS             5298..6056
                     /gene="deoR"
                     /locus_tag="RH057_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HDV3129635.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTQRIKLMY"
     gene            complement(6103..7305)
                     /gene="dacC"
                     /locus_tag="RH057_003307"
     CDS             complement(6103..7305)
                     /gene="dacC"
                     /locus_tag="RH057_003307"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HDV3129636.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDAWATGN
                     PALRGSSVMFLKPGDQVSVADLNKGVIIQSGNDACIALADYVAGSQESFIGLMNGYAK
                     KLGLTNTTFQTVHGLDAPGQFSTARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQP
                     NRNRLLWSSNLNVDGMKTGTTAGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEK
                     LLTWGFRFFETVTPIKPDATFVTQRVWFGDKSEVNLGAGEAGSVTIPRGQLKNLKASY
                     TLTEPQLTAPLKKGQVVGTIDFQLNGKSIEQRPLIVMENVEEGGFFGRMWDFVMMKFH
                     QWFGSWFS"
     gene            7552..8178
                     /gene="gstB"
                     /locus_tag="RH057_003308"
     CDS             7552..8178
                     /gene="gstB"
                     /locus_tag="RH057_003308"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HDV3129637.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(8175..9290)
                     /gene="yliI"
                     /locus_tag="RH057_003309"
     CDS             complement(8175..9290)
                     /gene="yliI"
                     /locus_tag="RH057_003309"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
                     /protein_id="HDV3129638.1"
                     /translation="MHRQSFFLVPLICLSSALWAAPATVNVEVLQDKLDHPWALAFLP
                     DNHGMLITLRGGELRHWQAGKGLSAPLSGVPDVWAHGQGGLLDVVLAPDFAQSRRIWL
                     SYSEVGDDGKAGTAVGYGRLSDDLSKVADFRTVFRQMPKLSTGNHFGGRLVFDGKGYL
                     FIALGENNQRPTAQDLDKLQGKLVRLTDQGEIPDDNPFIKESGARAEIWSYGIRNPQG
                     MAMNPWSNALWLNEHGPRGGDEINIPQKGKNYGWPLATWGINYSGFKIPEAKGEIVAG
                     TEQPVFYWKDSPAVSGMAFYNSDKFPQWQQKLFIGALKDKDVIVMSVNGDKVTEDGRI
                     LTDRGQRIRDVRTGPDGYLYVLTDESSGELLKVSPRN"
     gene            complement(9401..9784)
                     /gene="bssR"
                     /locus_tag="RH057_003310"
     CDS             complement(9401..9784)
                     /gene="bssR"
                     /locus_tag="RH057_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HDV3129639.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            9997..11322
                     /gene="rimO"
                     /locus_tag="RH057_003311"
     CDS             9997..11322
                     /gene="rimO"
                     /locus_tag="RH057_003311"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HDV3129640.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ADMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLVSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(11369..12697)
                     /locus_tag="RH057_003312"
     CDS             complement(11369..12697)
                     /locus_tag="RH057_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HDV3129641.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLAMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVISGTSCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKIYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(12705..>14823)
                     /locus_tag="RH057_003313"
     CDS             complement(12705..>14823)
                     /locus_tag="RH057_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CHASE9 sensor domain-containing protein"
                     /protein_id="HDV3129642.1"
                     /translation="TEEIQRAVILKMEMMPFLDSVGLVLDDNKYYLFSRRANDKIVVY
                     HQEQVNGPLVDESGRVIFADFNPSKRPWSVASDDSNNSWNPAYNCFDRPGKKCISFTL
                     RINGKDHDLLAVDKIHVDLNWRYLNEYLDQISANDEVLFLKQGHEIIAKNQLAREKLI
                     IYNSEGNYNIIDSVDTEYIAKTSAVPNNALFEIYFYYPGGNLLNASDKLFYLPFAFII
                     IVLLVVYFMTTRVFRRQFSEMTELVNTLAFLPDSTDQIEALKIREGDAKEIISIKNSI
                     AEMKDAEIERSNKLLSLISYDQESGFIKNMAIIESNNNQYLAVGIIKLCGLEAVEAVF
                     GVDERNKIVRKLCQRIAEKYAQCCDIVTFNADLYLLLCRENVQTFTRKIAMVNDFDSS
                     FGYRNLRIHKSAICEPLQGENAWSYAEKLKLAISSIRDHMFSEFIFCDDAKLNEIEEN
                     IWIARNIRHAMEIGELFLVYQPIVDINTRAILGAEALCRWVSAERGIISPLKFITIAE
                     DIGFINELGYQIIKTAMGEFRHFSQRASLKDDFLLHINVSPWQLNEPHFHERFTTIMK
                     ENGLKANSLCVEITETVIERINEHFYLNIEQLRKQGVRISIDDFGTGLSNLKRFYEIN
                     PDSIKVDSQFTGDIFGTAGKIVRIIFDLARYNRIPVIAEGVESEDVARELIKLGCVQA
                     QGYLYQKPMPFSAWDKSGKLVKE"
BASE COUNT         3776 a         3734 c         3475 g         3838 t
ORIGIN      
        1 tttttactct aacttattgt tttcattaac ccgtttacat aaatgggttt tttgttatct
       61 aaaacttatc tactaccctg caaccctctc aaccatcctc aaaatctcct cgcgcgatag
      121 cggcttacgg tcggtgacaa aatgcagcgt catcccctca ataaacgcat caagcgcgcg
      181 ggcggttccg ggttcaaacc attgttcgag cgtttgctga ctgcgctgca tccagttttg
      241 cattaccgtt tttaatagcg gttttcggct agccagcgcg tagagctggt acatcagctc
      301 catattatcc ggcgttgcaa cctgtgagct gtagatcata tcggtgatag cctggcatgc
      361 gccttgagca tcactaacat cgctaaaaaa tgcctgatat tgccgggaca taatctcagt
      421 aaaactgctg aacgcctcca gcaacaactc atcaattcct gaaaaatagt aggtcatcga
      481 tcccaacggt accccggcaa gggtagcaat tttgcggtgc gtaacagcat gtattccgta
      541 aagtttcacc gcctccagcg tggcctggat aattttttct cgccgttgcg gatcgttagc
      601 gcgacgcatg tcatttcctc tctctgcaat tgtgtacaaa tgtacacaac cttgctaatg
      661 ttgtcttccc tcttcttatt ttgacgtgat ctatgaccgt aaattcttca cgtaatgcat
      721 tgaaacgccg aacctgggcg ctgtttatgt tcttcttttt gccaggcctg ttaatggcgt
      781 cttgggcaac ccgtacgcct gctatccgcg atattctctc tgtctcgatc gctgaaatgg
      841 gcggtgttct ctttggtctg tcgatcggtt cgatgagcgg tattctctgc tcggcgtggt
      901 tagtgaaacg ctttggcacg cgtaatgtca tcctggtcac gatgtcctgc gcattgatcg
      961 ggatgatgat attaagtctg gcactctggc tgacatcgcc cctgctcttt gccgttggtc
     1021 tcggcgtctt tggggcaagt tttggttctg cggaagtggc gataaacgtt gaaggtgccg
     1081 ccgttgagcg agaaatgaat aaaacggttt tgccaatgat gcacggtttt tatagcctgg
     1141 gcacgctggc aggcgctggt gtcgggatgg cactgacggc ctttggcgtt ccggcaacgg
     1201 tgcacatttt attggcggcg ctggtaggta tcgcgcctat ttatatcgcc attcaggcaa
     1261 tccctgacgg tacgggcaaa aatgctgccg atggcaccca gcatggcgaa aaaggcattc
     1321 ctttttatcg cgatatccag ttgctgctga taggtgttgt ggtgctggcg atggcctttg
     1381 ccgaaggttc tgccaacgac tggttaccct tattaatggt tgacggtcac ggttttagcc
     1441 ctacttccgg ctcgctgatt tatgccggtt ttaccctggg gatgaccgtt ggacgcttca
     1501 ccggcggttg gttcatcgac cgttacagtc gcgttgccgt ggttcgggcc agtgcactaa
     1561 tgggggcgtt gggtattggg ctgattattt ttgtcgatag cgcctgggtc gctggggtgt
     1621 ctgttgtact ctggggactg ggtgcctcgc tgggcttccc gctgaccatt tctgccgcca
     1681 gcgataccgg ccctgatgca ccaacccgcg tcagtgtggt agcaacgacc ggttatctgg
     1741 ctttcctcgt cgggccgccg ctgctgggct atctcggcga acattatgga ttacgtagtg
     1801 caatgctggt tgtactggcg ctggttattc tcgcggctat tgtcgcgaaa gccgtcgcca
     1861 aacccgatac caaaacgcag acggcgatgg agaatagttg atgagcatta aattaattgc
     1921 ggtagacatg gatggtactt tcttaagcga tcaaaaaacc tataaccgtg agcggtttat
     1981 ggctcagtat cagcaaatga aagcacaagg aattcgcttt gtagtcgcca gcgggaatca
     2041 atattatcag ttgatctctt tcttccctga aattgctaat gaaattgcct ttgtggctga
     2101 aaacggcggc tgggtagtga gcgaaggcaa agatgttttt aatggcgagc tgtcgaagga
     2161 tgcgtttact actgtcgtgg aacatttgct gacgcgcccg gaagtggaaa ttattgcctg
     2221 cggaaaaaat agcgcctata cgctcaaaaa atatgacgat gccatgaaaa cggtggcgga
     2281 aatgtattat caccgtctgg aatacgtcga taactttgac aacttagagg atatcttctt
     2341 taagtttggc ctgaatcttt ccgatgaact gattccacaa gtacaaaaag cattacatga
     2401 ggccatcggc gatattatgg tgccggtcca caccggcaac ggcagcatcg atctgattat
     2461 ccccggcgta cataaagcca atggccttcg ccaactgcag aaattatggg gaatagacga
     2521 cagcgaagtg gtggtctttg gcgatggcgg taacgatatt gagatgctgc gtcaggcagg
     2581 ctttagtttt gcaatggaaa atgccggcaa tgcggtcgtc gcagcggcaa aataccgggc
     2641 aggctccaat aaccgtgaag gcgtactgga tgtgatcgat aaagtgctta aacacgaagc
     2701 gccatttgac caataaaaac ggcagcacgc ccggcaacac aatacctgaa tgaaggctgc
     2761 ataattcgcg gaaattaacc cgctgcatta tttcaggcga aatgtttatg aaaaattgtc
     2821 tactgttggg cgcgctttta atgggcttta ctggtgtggc gatggcgcaa agtgtcaccg
     2881 tcgatgtgcc gagtggatac aaagtggttg tggttcctga ttcagtcagc gttccacaag
     2941 cggtcagcgt cgcgactgtg ccgcaaaccg tctatgtggc tcctgcgcca gctccggctt
     3001 accgcccgca tccttatgtt cgccatcttg ccagcgtagg tgaagggatg gttatcgaac
     3061 accagattga cgaccatcac cattaattcg agaatgcctg atcgcacaaa gcaatcaggc
     3121 atttttttac ccttcgtgag aatttcccat ctgtttatct tttaaaaaga taaccatcag
     3181 cgacagccac aaaattccgt tgacaaggtt gaagagatta aacagtccgt tgcccccgtt
     3241 cagccaggca tgtttgctga tttcaatacc gacggtaaag atcagcattt gcagcattcc
     3301 catcgccgca gaaaccgtac ctttactcat atcgctggca aacagggtta atcgcaccag
     3361 tcccgcattc gccagaccaa tgccgaaagc ataaatactt aacccggcgg tcatccataa
     3421 atacgcgtgc gatgagataa ccgttgccgc agcagcgacc aacagaccaa taataatcgg
     3481 ccagccgccc ataataatca gcgaacgtac ggtgcggcgc gaggtcagac gcgctaacag
     3541 caagttaccc gcaattaacg ccccgaaaat aggcacttgc agcaagccat attcatagct
     3601 gctcaactgc tcgccggtaa tgatgataat cggcgactgg gcgatccacg ccagcaatgg
     3661 caggctaacg aatcccagcg ccagcgcccc cgccacaaag cggccgttct tcagcaccag
     3721 cttatagtca cgaccgagtt ctttcagtga cagtttctcg cctatacgcg tggcggtttc
     3781 aggcatggct tgttgcagac cgaaaaagga gatcgctgcc aatgcggcaa acaagacaaa
     3841 catcccttcc cagggcagca catggatcca cgccgcgccc accagcggac caagtagcgg
     3901 agcaatcagc gccacgttcg ccatcagcgc ggtgatcttg atacaaaccg cctcttcgaa
     3961 ggattcctga attgcggcgt atcccacagc gccaatgaaa cagaggctta tgccctgcaa
     4021 gaagcgcaac agagtgaatt gttcaatgtt ttgcgccagt aatattgcca gacaggtgat
     4081 gataaaccac accacccccg ccagcatcac cggacggcga ccaatacgat ccgacagcgg
     4141 ccccaggagc cattgtaaaa acatcccgcc cgccagatat gcggtcattg aagtaggaac
     4201 ccaatcaatg cccgcctgat attgttccac cacggccaac ataccgggtt gaatcatatc
     4261 gttgccgata taggttgaaa attcgtaaag caccagacag agagggaaaa gtaacgcctg
     4321 acgtccaagc ctggcaccgg aagctaattt attttgcatg caatttcttc gccaataata
     4381 atcgcgcaga gtttaataaa agcacagcta acgagaaagc gaattttgta gctgaaacca
     4441 cggttaagca cattcttaca ttattgcgag tatagctacg ctttctttaa gttttattta
     4501 acctatgccc gttacaatta cccaccgtaa acaggccgct tgagggaaat aagacgatgc
     4561 cgctttaccc agtttaacct gcactttatt ctcaacgact tgcctgtatt ggctcccttt
     4621 taatcacttt gcgtcgggaa gttatgctgg aaaatttgaa tctctctcta ttctctctta
     4681 ttaacgcgac gccagactcg gctccgtgga tgatctcgtt ggcgattttt attgctaaag
     4741 atttgattac cgtggtaccg ctgctggccg cggtgctttg gttgtggggg cttacagcac
     4801 aacggcaact ggtgataaaa atcgctatcg cgctggcggt cagcctgttt gtgtcctgga
     4861 cgatgggaca tctttttccg cacgaccgac cctttgtcga aaatatcggc tataacttcc
     4921 tgcatcatgc ggcggatgac tcattcccaa gcgaccacgg tacggtgatt ttcacctttg
     4981 cactggcatt tttatgctgg catcgcctgt ggtccggctc acttttaatg gtgctggccg
     5041 tcgtcattgc ctggtcgcgc gtttatcttg gcgtgcactg gccgctggat atgctcggtg
     5101 gattgctggc aggtatgatt ggctgcctta gcgctcagat tatctggcaa gcgatggggc
     5161 ataaactcta tcaacgtctg caatcgtggt atcgcttctg ttttgcatta ccgatccgca
     5221 aaggctgggt gcgtgactga tctctgctaa aaagtgtagt attgagcggc tcgcttcaat
     5281 aactattcag agggattatg gaaacacgtc gcgaagagcg tatcgggcag ctgctgcaag
     5341 aattaaaacg cagcgataag ttacatctta aagacgccgc cgccctgctt ggggtttcgg
     5401 agatgacgat tcgtcgcgat ctgaacaacc acagtgcgcc cgtcgttttg ctcggcggct
     5461 atattgttct ggaaccgcgc agtgccagcc attatctgtt aagcgatcaa aaatcccgcc
     5521 tggtggaaga aaaacgccgg gcggcaaaac tggctgcgac gctggtagaa cccgatcaga
     5581 ccctcttttt tgactgtggc accaccacgc cgtggattat tgaagcgatt gataatgaaa
     5641 tacctttcac cgccgtttgt tattcgctaa atacctttct ggcgctgaaa gagaaacccc
     5701 attgccgcgc atttctgtgc ggtggtgaat ttcacgccag caacgccatt ttcaaaccca
     5761 tcgattttca gcaaacgctg aataattttt gcccggatat cgctttttat tctgcggcgg
     5821 gcgtgcatgt cagtaaaggc gctacctgtt ttaatcttga agagttgccg gtaaaacact
     5881 gggccatgtc gatggcgcaa aagcatgtgc tggttgtcga ccacagtaaa tttggcaagg
     5941 tgcgtccggc gcgcatgggt gacctgaaac gctttgatat tgtggtgagc gattgttgcc
     6001 cggaagatga gtatgtgaag tacgcgcaga cgcagcgcat taagttgatg tattaatgac
     6061 gtataaccgg atgacgtttc gcgccatccg gttatcagaa gattaagaga accagctgcc
     6121 gaaccactga tggaatttca tcatcacgaa atcccacatc cgaccaaaga atccgccctc
     6181 ttccacattt tccatcacga tcagcggacg ctgctcaatg gatttaccgt taagctggaa
     6241 atcaatggtc ccgacaacct gacctttttt cagcggtgcg gtaagctgcg gttccgttaa
     6301 cgtataactc gctttcaggt ttttcagctg cccacgcggt atggtcactg agcccgcttc
     6361 acctgccccg agattcactt cgctcttatc accaaaccag acgcgctgag tcacaaaggt
     6421 ggcatcaggt ttaattggcg tcacggtttc aaagaagcgg aaaccccagg tcaataattt
     6481 ctcagactca ttaaaacgga tacggtcggt tttcgccccc aacactacgg agattaaacg
     6541 catatcgccc tgggtagccg aagcaaccag attatatccc gcgcctgccg tggttcctgt
     6601 cttcatgcca tcaacattca gattgctgct ccacagcaga cggttacggt taggctgacg
     6661 aattttgttg aaggtgaact ctttctcttt atgaatggcg tactcttccg gcacatcgtg
     6721 gatcaatgct ttacccagca atgccatatc gcgcgcggtg ctgaactgcc ccggcgcatc
     6781 caggccgtgc accgtctgga aggtagtgtt ggtcagaccc agttttttgg cataaccatt
     6841 catcagacca ataaatgact cctggctccc ggcaacgtaa tcagccagcg caatacaggc
     6901 gtcattaccg gactggataa tcacaccttt gttcaagtct gccaccgaaa cctgatcgcc
     6961 cggtttgagg aacattaccg atgaaccacg cagtgccgga tttcccgtcg cccaggcatc
     7021 tttaccgacc gtcaccatat cggtgagttt aatcttatcg gccttaagcg cctgcccaac
     7081 cacatagctg gtcatgattt tagtcaggct cgcgggatcc agtttctcat ccgcattgcc
     7141 ttctgccagc actttaccgc tggcgtaatc cattaaaatc catgcacgcg catccacgct
     7201 cggcgcttca acggtttgtt cggccgcgaa tgccgttggg gcaaaaagga ataaaaatgc
     7261 agaacccgct gcaagaccac gaaggagaga ggagtattgc gtcataaatg ccacccgagt
     7321 atccattcaa aaaaaatacg ttatatcgcc gtatgataag ttcggtgatt aataacgcac
     7381 acacaaactt aaagaaaccg taagttggta aagtttttaa agtttatgca atatcctgcc
     7441 gcttcgctgc ttctggtgtc atttttttgc cagctgttgc ttaaaagtta actttacttc
     7501 accatcggag tcctgttcag tctgtggctg cctgtcggaa aaggggtgag tatgattacg
     7561 ctgtggggtc ggaataattc aaccaacgta aaaaaagtat tgctgacgct cgaagaactg
     7621 gaactacctt atgagcaaat tctcgcgggc cgtgagtttg ggataaatca cgatgctgat
     7681 tttctggcga tgaaccctaa cgggctggtg ccgttgttgc gtgacgacga aagtgatctc
     7741 attctttggg aatcaaacgc cattgtccgc tatctggcgg cacagtacgg gcaaaaacgc
     7801 ctgtggatcg actcaccggc acgtcgtgcg gaagcggaaa aatggatgga ctgggcaaac
     7861 cagacactca gtaatgctca tcgcgggatc ctgatgggat tagtcagaac accacccgaa
     7921 gagcgcgatc aggccgccat tgatgccagt tgcaaagagt gcgacgccct gtttgccctg
     7981 ctcgatgcgg aactggcaaa agtaaaatgg ttctctggcg acgagtttgg tgtgggcgat
     8041 atcgctatcg caccgttcat ctacaatttg tttaacgttg gcctgacctg gacaccgcgt
     8101 ccaaatctgc aacgctggta tcagcaactc actgaacgcc ccgcagtgcg caaagtggtg
     8161 atgattcccg ttagctaatt gcgtgggcta actttaagta attccccact ggactcgtcg
     8221 gtgagaacgt ataaataacc gtcgggtcca gtgcgaacat cacgaattcg ctgccctctg
     8281 tccgttaaaa tacggccatc ttctgtcact ttgtcgccgt tgacgctcat cacaatgaca
     8341 tctttatctt tcagcgcgcc tataaataat ttttgctgcc actgggggaa tttatcgctg
     8401 ttatagaagg ccatgccgct cacagcgggc gaatctttcc agtaaaaaac aggttgctcg
     8461 gtcccggcga cgatctcccc tttcgcttcc ggtatcttaa agcctgaata gttgattccc
     8521 caggttgcca gcggccagcc gtagtttttg cctttttgcg ggatattaat ttcatcacca
     8581 ccgcgcgggc catgttcatt cagccacagt gcattactcc acggattcat cgccattcct
     8641 tgcggattac gaatgccata agaccagatc tcggcgcgcg caccggattc ctttataaaa
     8701 ggattatcat ccgggatttc gccctggtcg gtcagacgca ccagtttgcc ctgtaattta
     8761 tccagatcct gcgccgtcgg gcgctgattg ttttcgccca gagcaataaa aagataacct
     8821 ttaccgtcga ataccagccg cccgccaaaa tggttgccgg tagacagttt tggcatctgg
     8881 cggaagacgg tgcggaagtc ggccactttt gagagatcat cacttaagcg gccataaccc
     8941 acagcagttc cggctttgcc atcatcgcca acttcggaat aacttaacca gatgcggcga
     9001 gactgagcaa aatcaggcgc taaaaccacg tccagcaggc cgccctgccc gtgcgcccaa
     9061 acgtccggaa ctccggaaag cggcgcagat aatccttttc ctgcttgcca gtgacgcaac
     9121 tcgccgccgc gcagagtgat taacataccg tgattatcgg gtaaaaaggc cagtgcccag
     9181 ggatggtcga gtttgtcttg cagtacttcg acatttaccg ttgcaggagc cgcccagaga
     9241 gcggaagaaa gacaaataag gggcacaagg aaaaaggatt gtcgatgcat aacgcctccg
     9301 gattgactct ggcttaaagc gtagtcagtg gaggagataa caaattcatt tttacaaaaa
     9361 cttaaacatg aagggggaga cgctttctcc cccttagttt tcaggccttc tcaagcatgg
     9421 cgtgcttctg caggctctgg atactcagcg ttaagctcat cagacaattt tcaagcttat
     9481 cggcgttgac ggtaataaca gtcgggcaat catggtgccc actcatcaaa catactgcgg
     9541 ctgtcgctaa tgcttcttca gcatgatgaa gagcactcca ctcttcctga tccagatgaa
     9601 gattcaaccg cagcgattta tcgtgcagtt cgcgattcag tttaaaaaag ttatctcgta
     9661 gatgattgct ttcgctgacg gacatgtatc cttttgcctt cctcagttgc acgtatgcag
     9721 cgaggtcaac gcgtgcgtcg atcaaccggt tcagcagatc gattcgctgt ctgtcaacga
     9781 acatacttcg atcctcctct ttccagcctt gttgcaatta aatagtatgg ctattttttg
     9841 agcaaaatgc tgtccaggat caattcttca cgccaaatta acaaaattta catttccttc
     9901 gcctccgcca gcgaatgaga ttgcgataca aattttatcc agaactctgt agaatccctg
     9961 ccctgacctt tcctgtaact gaatattttt taagctatga gcaaagtaac tccccagccg
    10021 aaaatcggct ttgtttccct tggctgtccg aaaaaccttg tcgattcaga gcgtattctc
    10081 accgaactac gcactgaagg ttatgacgtg gtaccgagct atgacgatgc ggacatggtg
    10141 atcgtcaaca cctgcggctt tattgacagc gcggtacaag aatcactgga agccattggt
    10201 gaagcgttga atgaaaacgg caaggtaatt gtgaccggtt gtctgggggc aaaagaagat
    10261 cagatccgcg aagtccaccc gaaagtgctg gaaatcaccg ggcctcatag ctatgagcag
    10321 gttctggagc acgttcatca ctacgtgcca aaaccgaaac acaacccatt cctgagcctg
    10381 gtgccagaac aaggtgtgaa actgacaccg cgtcattatg cctatctgaa aatttcagaa
    10441 ggctgtaatc accgctgcac cttctgcatt attccgtcta tgcgcggcga cctggtgagc
    10501 cgtccgattg gcgaagtgtt aagtgaagcg aaacgtctgg tagatgcggg cgttaaagag
    10561 attctggtga tctcgcagga tacttccgcc tatggcgttg atgttaaaca tcgtactggc
    10621 ttccacaacg gcgagccggt aaaaaccagc atggtcagcc tgtgcgaaca gttatcgaaa
    10681 ctggggatct ggacacgtct gcactacgtt tacccttatc cgcatgtgga cgacgtcatc
    10741 ccactgatgg cagaaggcaa aatcctgccg tatctggaca ttccgttgca gcacgccagc
    10801 ccgcgcattc tcaaactgat gaagcgtccg ggttctgtag atcgccaact ggcgcgcatc
    10861 aaacagtggc gcgaaatctg cccggaactg accctacgct caacctttat tgtcggcttc
    10921 cctggcgaga cagaagaaga tttccagatg ctactagact tcctgaaaga agcgcgtctg
    10981 gatcgcgttg gctgctttaa atacagcccg gttgaaggtg cagacgccaa tgccctgcct
    11041 gaccaggttc cggaagaagt taaagaagaa cgctggaacc gtttcatgca gctgcagcag
    11101 cagatttccg ccgagcgcct gcaagagaaa gtgggccgtg aaattctggt gattatcgac
    11161 gaagtggacg aagaaggcgc gattggtcgc agcatggcag atgcaccgga aatcgacggc
    11221 gcggtttatc tcaatggtga aaccaacgtt aagccgggtg atatcctgcg tgtgaaagtc
    11281 gagcacgccg atgagtacga tttgtggggt agccgggttt aattaattca ggccgggcaa
    11341 aatcatttgt ccggcctgcg aataatgatt actgccagct aatctgagcc ctgccgtttt
    11401 tcttactctt atacagccgc tcatccgcca gcattttcaa gcgttcgacg gtggtacatt
    11461 catttgaatg cgctacaccg atactggtac ttaaattaat gcttcccacc tggaaatgaa
    11521 cgatttcttt tagaatcgat agcaactctt ccagcgttcc acccgtataa atcaccgcaa
    11581 attcgtcacc gccgaagcgg tagattttac ctttatctac cgggatctgt gatttaagga
    11641 ttttcaccac tttcatcaac acttcatcgc ccagcaaatg accgaaggtg tcgttaatgt
    11701 ttttgaattt atcgacgtca aacaggcaca aataaccgcc tttttccggc agcgctttta
    11761 aatcttcgtc aaaggatttt ctgcccattg ctttggtcag gtcgtccagg aacgccgagt
    11821 tatacatact gtagagtttt ttgacatcgg cagccgaaaa gaaaagcccg ccatccagta
    11881 cgcgttcaaa atggttatta atgatggctt ctttctccac aataccacga ttaatcactc
    11941 tgcgtaccag atagaggcaa ctggtacccg atatcactgc caccaatgcg gcaaagaaaa
    12001 taatcaatgt acagacccgc aaataaacat cttttttata cgcaaatacg tacaatccgg
    12061 tcggcaaata ggcgaagtgc tccatcgctt ccgaaacggt gaagatggcg tttttagcat
    12121 tatattcatc accggtcacc ggtttaccgt tcttaaatag agagaacatc aggttctcgc
    12181 tgtccatcgc cagcgatttt gacagtaaag aaaaacgctc cagaccaagc aaaaatatca
    12241 gactacgatt gtgagttacc ggatagaggt aatagacgaa ggttttaccg tcaaaagaga
    12301 taaaccctgt atttttgaca tgtcgatact tcacaacctg cataatatct gcacaataat
    12361 ttttctcgaa ctcctttacc tgaatacatt cgtcactgga aatcagataa ggaccgacat
    12421 cgtacaaata agagggggaa accgagtcgg cgtaagctgt gcgcaaacca accaacagct
    12481 gttcctccgt gggatttagc gtgttgataa gatcaattgc cacgctacgc cgatattcag
    12541 caatatttgc caccgcaaaa gcagtatcaa taacggactg tttcaggcta tacacccgtt
    12601 cctttaccat ttgagtgtag atcccgcagg caactgctga aatcatgata aaaatcagca
    12661 gactgactgt aagtacgaac ttattgattc tggacatacg taaactactc ttttactaat
    12721 tttccacttt tatcccaggc ggagaatggc atgggtttct ggtacagata cccctgagcc
    12781 tgaacacatc ctaatttaat taattcgcgc gcaacgtctt cgctctctac gccttccgca
    12841 atcaccggga tccggttata gcgtgccaga tcgaaaataa tgcgcacaat ttttcccgca
    12901 gtaccgaaaa tatcgccggt aaattgcgag tccaccttta tgctgtctgg attaatttca
    12961 taaaaacgtt tcaggtttga caaaccggtg ccaaagtcat caatcgatat ccgtacccct
    13021 tgtttacgca gttgttcaat attgagataa aaatgttcat taattcgctc gatcacggtt
    13081 tcagtgatct caacacagag gctgttcgcc ttcaggccat tttctttcat gatggtggta
    13141 aaacgctcat gaaagtgcgg ttcgtttaac tgccagggcg aaacattaat atgcagtaag
    13201 aaatcatcct tcagcgacgc acgctgacta aaatgtctga attcacccat tgccgtttta
    13261 ataatctgat aacccagctc attgataaac ccgatatctt cagcaatggt aatgaacttc
    13321 agcggtgaaa tgatcccccg ctccgcagaa acccaacggc acaacgcctc cgcgcccaga
    13381 atggcgcggg tgttaatatc aacgatcggt tgatagacga ggaatagttc gccaatttcc
    13441 attgcatggc gaatattacg cgcaatccag atattctctt ctatttcgtt gagtttcgcg
    13501 tcatcacaaa aaataaactc ggagaacata tggtcacgga tactggaaat cgccagtttc
    13561 agtttttctg cgtaactcca ggcgttttcc ccctgcaaag gttcacaaat ggcagactta
    13621 tggatgcgca gattgcggta gccaaagctg ctgtcaaaat cgtttaccat cgctatttta
    13681 cgggtaaatg tctgtacatt ttcccgacac agaagtaaat agagatcggc attgaatgtc
    13741 acgatatcgc agcattgcgc atatttctcg gcaattcgct gacacaattt cctgacgatt
    13801 ttattgcgtt catcaacacc aaacaccgct tccacggctt ccagaccaca cagtttgatg
    13861 atccccacag ccagatactg attattgttg gactcaataa tcgccatatt tttaataaaa
    13921 ccgctttcct gatcgtaaga gatcagtgag agcaatttat ttgaccgttc aatttcggca
    13981 tctttcattt ccgcgatcga atttttgatg ctgataatct ctttcgcatc gccttcgcga
    14041 attttcagag cctcgatttg atccgttgag tcgggcaaaa acgccagcgt attaaccagc
    14101 tcggtcattt cagaaaattg ccgacggaac acacgagtgg tcatgaaata taccaccagc
    14161 aacacgataa taatgaaagc aaacggtaga taaaaaagtt tatctgatgc gttcaataaa
    14221 ttaccgccag gataataaaa atagatttcg aacaatgcat tatttggcac tgctgatgtc
    14281 tttgcaatat actccgtatc gacagaatca ataatattat aattaccttc gctattataa
    14341 ataatcagtt tttcacgagc gagttgattc ttggcaatga tctcatgacc ttgtttcaaa
    14401 aatagaactt catcattagc gctgatttga tcaagatact cgttcagata ccgccagttt
    14461 aaatcgacat gaattttatc taccgctaac aaatcgtgat ctttgccgtt gatacgtagc
    14521 gtaaaagaga tacatttttt acccggacga tcaaagcaat tgtatgccgg attccagctg
    14581 ttgttagagt catctgaagc caccgaccac ggtcgtttcg atgggttaaa atcggcaaaa
    14641 ataacccgcc ctgactcgtc gacaagcggt ccatttactt gttcctgatg ataaacaacg
    14701 attttatcat tcgccctccg cgaaaagaga taatatttat tatcatcaag taccagtccc
    14761 accgagtcta aaaatggcat catttccatt ttcagaatca cggcacgttg aatctcctct
    14821 gta
//
LOCUS       DAPHDW010000110        14716 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.111, whole genome shotgun sequence.
ACCESSION   DAPHDW010000110 DAPHDW010000000
VERSION     DAPHDW010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14716
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.111"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(279..779)
                     /locus_tag="RH057_003314"
     CDS             complement(279..779)
                     /locus_tag="RH057_003314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HDV3129643.1"
                     /translation="MNNTDSLEKIIKHQQNNDPKYPCQEHLLIQLCIRVNKKIQDNIS
                     SLLSGYGINYSTYMVLTILSTADNHCLSPSKISRELQFTRTNITRITDFLEKAGYIER
                     TDSREDRRAKKISLTSDGLSFIQNVNIAQSLHLKEIWSPLTHAECELFEDINKKLLTH
                     IADVCP"
     gene            complement(1032..1241)
                     /locus_tag="RH057_003315"
                     /pseudo
     CDS             complement(1032..1241)
                     /locus_tag="RH057_003315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1409..1925
                     /locus_tag="RH057_003316"
                     /pseudo
     CDS             1409..1925
                     /locus_tag="RH057_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001751479.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1816..2163
                     /gene="tnpB"
                     /locus_tag="RH057_003317"
     CDS             1816..2163
                     /gene="tnpB"
                     /locus_tag="RH057_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3129644.1"
                     /translation="MITLPAGSRIWLVAGITDMRNGFNGLAAKVQNTLKDDPISGHLF
                     IFRGRRGRLVKLLWADSGGLCIYMKRLERGTFIWPVAREGKVYLTPAQLAMLLEGIVW
                     MQPGRSKSNSLRI"
     gene            2273..3792
                     /locus_tag="RH057_003318"
                     /pseudo
     CDS             2273..3792
                     /locus_tag="RH057_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050628.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(3802..5009)
                     /locus_tag="RH057_003319"
                     /pseudo
     CDS             complement(3802..5009)
                     /locus_tag="RH057_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
     gene            complement(5133..5969)
                     /locus_tag="RH057_003320"
                     /pseudo
     CDS             complement(5133..5969)
                     /locus_tag="RH057_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            5962..6612
                     /locus_tag="RH057_003321"
                     /pseudo
     CDS             5962..6612
                     /locus_tag="RH057_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(6790..7071)
                     /locus_tag="RH057_003322"
     CDS             complement(6790..7071)
                     /locus_tag="RH057_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016230754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129645.1"
                     /translation="MSESVKLSFYRVHECGYYLWGNNTPVFGSLQELLTDLHFWSTDK
                     SIENTKLYEPQADSDYLGTYLFNINRSGDYWLVTIWNEVPSTKRELLLL"
     gene            complement(7257..12767)
                     /locus_tag="RH057_003323"
     CDS             complement(7257..12767)
                     /locus_tag="RH057_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HDV3129646.1"
                     /translation="MINKNKVTERSGIHDTVKSLSENLRKYIEAQYHIRDEGLIAERR
                     ALLQQNETIAQAPYIEATPIYEPGAPYSELPIPEAASNVLTQLSELGIGLYQRPYKHQ
                     SQALESFLGENASDLVIATGTGSGKTESFLMPIIGKLAIESSERPKSASLPGCRAILL
                     YPMNALVNDQLARIRRLFGDSEASKILRSGRCAPVRFGAYTGRTPYPGRRSSRRDELF
                     IKPLFDEFYNKLANNAPVRAELNRIGRWPSKDLDAFYGQSASQAKTYVSGKKTGKQFV
                     LNNWGERLITQPEDRELMTRHEIQNRCPELLITNYSMLEYMLMRPIERNIFEQTKEWL
                     KADEMNELILVLDEAHMYRGAGGAEVALLIRRLCARLDIPRERMRCILTSASLGSIED
                     GERFAQDLTGLSPTSSRKFRIIEGTRESRPESQIVTSKEANALAEFDLNSFQCVAEDL
                     ESAYAAIESLAERMGWQKPMIKDHSTLRNWLFDNLTGFGPIETLIEIVSGKAVKLNIL
                     SENLFPDSPQQIAERATDALLALGCYAQRASDGRVLIPTRMHLFYRGLPGLYACIDPD
                     CNQRLGNHSGPTILGRLYTKPLDQCKCASKGRVYELFTHRDCGAAFIRGYVSSEMDFV
                     WHQPNGPLSEDEDIDLVPIDILVEETPHVHSDYQDRWLHIATGRLSKQCQDEDSGYRK
                     VFIPDRVKSGSEITFDECPVCMRKTRSAQNEPSKIMDHVTKGEAPFTTLVRTQISHQP
                     ASRPIDGKHPNGGKKVLIFSDGRQKAARLARDIPRDIELDLFRQSIALACSKLKDINR
                     EPKPTSVLYLAFLSVLSEHDLLIFDGEDSRKVVMARDEFYRDYNSDLAQAFDDSFSPQ
                     ESPSRYKIALLKLLCSNYYSLSGTTVGFVEPSQLKSKKMWEDVQSKKLNIESKDVHAL
                     AVAWIDTLLTEFAFDESIDSTLRIKAAGFYKPTWGSQGRFGKALRKTLIQYPAMGELY
                     VEVLEEIFRTHLTLGKDGVYFLAPNALRLKIDLLHVWKQCNDCTALMPFALEHSTCLA
                     CGSNSVKTVEPSESSYINARKGFWRSPVEEVLVSNSRLLNLSVEEHTAQLSHRDRASV
                     HATTELYELRFQDVLINDNDKPIDVLSCTTTMEVGVDIGSLVAVALRNVPPQRENYQQ
                     RAGRAGRRGASVSTVVTYSQNGPHDSYYFLNPERIVAGSPRTPEVKVNNPKIARRHVH
                     SFLVQTFFHELMEQGIYNPAEKTAILEKALGTTRDFFHGAKDTGLNLDSFNNWVKNRI
                     LSTNGDLRTSVAAWLPPVLETGGLSASDWFAKVAEEFLNTLHGLAEIVPQTAVLVDEE
                     NEDDEQTSGGMKFAQEELLEFLFYHGLLPSYAFPTSLCSFLVEKIVKNIRGSFEVRTV
                     QQPQQSISQALSEYAPGRLIVIDRKTYRSGGVFSNALKGELNRARKLFNNPKKFIHCD
                     KCSFVRDPHNNQNSENTCPICGGILKVEIMIQPEVFGPENAKELNEDDREQEITYVTA
                     AQYPQPVDPEDFKFNNGGAHIVFTHAIDQKLVTVNRGKNEGESSGFSVCCECGAASVY
                     DSYSPAKGAHERPYKYIATKETPRLCSGEYKRVFLGHDFRTDLLLLRITVGSPLVTDT
                     SNAIVLRMYEDALYTIAEALRLAASRHKQLDLDPAEFGSGFRILPTIEEDTQALDLFL
                     YDTLSGGAGYAEVAAANLDDILTATLALLESCECDTSCTDCLNHFHNQHIQSRLDRKL
                     GASLLRYALYGMVPRCASPDIQVEKLSQLRASLELDGFQCIIKGTQEAPMIVSLNDRS
                     IAVGSYPGLIDRPDFQHDVYKSKHTNAHIAFNEYLLRSNLPQSHQNIRKMLR"
     gene            complement(12764..13807)
                     /locus_tag="RH057_003324"
     CDS             complement(12764..13807)
                     /locus_tag="RH057_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129647.1"
                     /translation="MEWRAVSRDKALDMLSTALNCRFDDEGLRISAVSECLRSVLYQY
                     SISETEEARQTVTSLRLTSAVRRKLVPLWPDIADIDNAIHPGIMSILNSLAELGDMIK
                     LEGGNWLTAPPHAVRIDNKMAVFFGGEPSCTFSTGVVAKSAGRVRLVEEKVCTGSVEI
                     WDANEWIGAPAEGNEEWSSRLLSGTISGFIDAPGNMSETTAYVRGKWLHLSELSFNKK
                     QIYLCRMSVDNHFSYYLGEIEAGRLCRMNSLESSDDVRRLRFFLDTKDNCPLKVRIKI
                     SNGLARLRLTRRLPRRETKVLLLGWRESGFENEHSGITHHVFPEEILPIVRSAFEGLG
                     IIWINEFTRRNEI"
     gene            complement(13819..>14716)
                     /locus_tag="RH057_003325"
     CDS             complement(13819..>14716)
                     /locus_tag="RH057_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001519245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129648.1"
                     /translation="EVSVTNRFTKFHEIAHYENYEYLSSSEDISNRISLNLQAVGLTK
                     NSAEKLARLTLATFVSGQIIQFSGSLADIIADAIAIAIGAPRYHIWRVPVGIISDMDA
                     FDFIETIAESSRCLLLKGANLSAFEIYGAAIRDIVVQRQIHPTNYDHLALIATWKQGP
                     ATFPDGGMLAELGPVIDTDTLKMRGLSATLPQLKPGCLAKDKWTNIDGLHLDSVDDYV
                     DELRALLDEAGFDGGTLWKRMIHIFYTSLIRIPNGNYIYDLYSVLSFYTLTWAKIKGG
                     PVQKIEDIANRELKNYSAKISS"
BASE COUNT         3899 a         3478 c         3288 g         4051 t
ORIGIN      
        1 gcaactattt ttattgcgcg ttaccccata tgagataagc tcaatcatta cgttatcttg
       61 tatcagtaag ttctacggtg aatcatgctg atatttgcgg gttaggcacc gcatcattca
      121 caagaaccgt cgtatgagtt tgtcattttg ctgcattgtg acctaaacca gtagttttct
      181 tgtatcgctg tttccgcctg tagttatgtc gctcttatgc ctgcttatta tatgattcat
      241 tgcttgaagc tcttcagttt cctcaaactc ttcatgcttt agggacacac atcagcaata
      301 tgcgtcagta attttttatt aatgtcttca aacagttcac actctgcatg ggtcagcggg
      361 ctccagattt ctttcaggtg cagactttgt gcaatattga cgttctgaat aaaggacaga
      421 ccatctgatg tcagactgat ttttttagca cggcgatcct ctctgctatc agttctttct
      481 atataccctg ctttttctaa aaaatctgta atgcgggtaa tgtttgttct ggtaaactgg
      541 agttctctgc ttatctttga gggcgaaaga caatgattat ctgctgtgga taatatggta
      601 agaaccatat atgttgaata atttattcca tatccactga gtaaactcga tatattatcc
      661 tgtatttttt tgtttacgcg gatacagagt tgtatcaaca gatgctcctg gcaggggtat
      721 tttggatcgt tgttttgttg atgtttgatt attttttcta atgagtctgt gttgttcata
      781 taatcatttc catttgctga aatactttgc ctttatacaa aagtgcatca gacatgcggt
      841 aagcatctac ttttcacaac aagttatgtc tgccagatat ctgtatgaat gagacaacca
      901 ggcagaaagg aaggataact tcattcgcat aaaaaaacat aaatgtttgt agtggtcaac
      961 aaaaaatgat cacgatttta aatttttttc cagtatctat tttccaattc gtttggtagt
     1021 aacctgccat tttattcgtg aggcctgagt gcactataat acccaacgat atagtccgtt
     1081 atgacttgaa ctgcatcgct gaagcttacg taccccacca ctggtaacca ttcgttcttt
     1141 agactcctga agatgcgttc cattgggctg ttatcccagc agtttcccta tcggctgata
     1201 ctttgcttta tcctgtaaca ccagagaagt tgttgatatt tcagttcagt atactgacct
     1261 cttgcctggc ttgatcaact ggtaaagtac ttcaccgttg agcttcccgt ggttgatttt
     1321 acgtaagcga cccgtcagag acgtcttcag gcagtcgagt gaaaacgcta acttagacgc
     1381 ccatctgaaa cagacggaca tctaagttat ggaatctgaa acccgggcct ggcggaccga
     1441 accccgccgc gtttattcca gcgaattcaa actcaggctg gtcgaactgg ctacccgacc
     1501 ggacgccaat gttgcggata ttgcccgtac gcacggggtc aacgataacg tcctttttaa
     1561 atggatccgt ctgtggcgta aagaaggccg ggtattccgc cggctgccag ctcccgttcc
     1621 atctgtatcc cctcctgcgg catttccggt cgaaattgta cctgactcca ccacccggca
     1681 gtctccggct ctgtctggtc agggaacggg gtcaacctgc tcgctgacgc ttcgccacgg
     1741 caccctcaca ctcgacaatc ctgacccgga gatacagctc cggatcgtcc gcgaactcgc
     1801 cggcaggagc acagtatgat tacgcttccg gctggcagcc gtatctggct cgtggccggc
     1861 atcaccgaca tgagaaacgg cttcaacggt ctggccgcaa aggtccagaa caccctgaag
     1921 gatgacccga tctccggcca tctcttcatc ttccggggac gcaggggcag gctggtaaaa
     1981 ctcctgtggg cagacagcgg agggctctgc atctatatga aaagacttga acggggaacg
     2041 ttcatctggc cggtcgcccg ggagggaaag gtgtatctga ccccggcaca gcttgccatg
     2101 ctgctggagg ggatagtctg gatgcaaccg ggaaggtcga aaagtaatag tctgcggatc
     2161 tgaatccctt tacctgggca ggatgaaaaa tatcccgctt cctgataata tcgaccagct
     2221 gaaagccctg ttgcgtcagc agcaggttgc tgtgtaacag ctaaatgtta ccatgaaaaa
     2281 gcagcaggcc cggctgcgcc agtacgccgg acaggttgcc gactacgaac aggaaatcga
     2341 acggctgaag gcacagcttg ataagttgat aagctgcggc ggatgctgtt cgggcagagt
     2401 tcagagaaaa gccgcaataa actggaaaat aaaatccgcc aggcagagaa gcggctggct
     2461 gagctggaaa accgtcttgc agcggcaaaa cgctgtctgc aggacgatga gcccactgtc
     2521 agtacggttg agcaggcaga gcccccggtg gcggtcgccc cacaggaccg gcctgcacgc
     2581 cgctcccgtc gtcagaaact gccggcagac ctgccacgtg aaacccggcg catcaccccg
     2641 acagtgactg tctgcccgaa atgcggcggc gacctgaagg tgatgagtga aaccgtctca
     2701 gagcaactgg agataatccg cagcgccttt aaggtcatcg aaacggtgcg cccgaagctg
     2761 gcctgcagcc gctgcgatgt gatagtccag gccccactcc tcccgaaacc tctgacccgc
     2821 agtgtggccg gtgcgggcct gctggcccgc atcctggtga gtaaatactg tgaccatacc
     2881 ccgctgcatc gtcagtccgt catcttcagc cgccagggtg tggaactgag ccgaaacacg
     2941 ctggtccggt gggtggcgat gaccgcagaa cgactcagtc ccctgcacga tgcgctgaac
     3001 cgctacgtga tggagagcag caaacagcac acggacgata ccccggtgaa agtgctggag
     3061 ccggggagcg gtaaaacccg gacgggccgg ctgtgggtgt atgtgcgcga tgaccggaac
     3121 agcggttcag cggctccgcc agcggcgtgg ttcgcttaca gtacagatcg tcggggctgt
     3181 cacccgcagg ggcatctggc ggagtggaaa ggtatcctgc aggcagatgc gtttgccggt
     3241 tacgatgcgc tttatgagtc agggctgatc agggaagccg cctgcatggc gcatgcaagg
     3301 agaaagatcc atgatgagca tgttcgccgg ccgacagcga tgacaacgga ggcgctgaag
     3361 cgtatagccg ggctgtatgc ggtggaagcg acaatccggg gtcaaccggc agagaaacgg
     3421 ctggcagaac ggcaggagaa aagcgtcccg cagatgaagg cgttgttcgc gtggctgaca
     3481 gaacagatgg agacgttgtc ggtgcatgca gagctgggga aagcgttcag ctatatgctg
     3541 aaccaccgtg aagcgcagtc gctctgttgt gaggacgggc gtgtggaact ggacaataat
     3601 atcgcagaaa acacactgcg ttgtgtcgcc ctatggcgac gcaactgtct attcttcggc
     3661 tcagacacgg gaggtcagag cgcagcgatc ctgtacagcc tgctggcgac atgccggctg
     3721 aatggagtgg atccggaaag ctggttgcgt gaggtaatca gctgtatcga cccagtttac
     3781 cattctgtgt aaatgccttt ctcagaagtg accgtcaagg cggtcaccga atttgataat
     3841 aaagcgactc atggctatcc gccagtcctg aagtggcatc gtccatttct gtgaggctga
     3901 ctggatagcc agccacacca cttttttcac cgattcatct gtctggaaca ctttccgttt
     3961 ctttatcgca tgtctgatga cgctattcag cgattcgatt gcgttagtgg tgtagataac
     4021 cttgcggatc gcctccgggt acacgaagaa ggtggcaaca ttcggccagt ttgcccgcca
     4081 actgcgactt atctgcgggt acagtgcatc acattccttc ccgaacgcat ccagcgcctg
     4141 aaacgcgcct tcttccgtgg ctgcgcggta aacctgtttc aggtcacggg ttaccgcttt
     4201 atagtctttc cacgccacga accgcaggct gttgcgtacc atgtgtacta tgcacaactg
     4261 gatctgcgtc tccgggtaga cggcattaat cgcttccggg aagcctttca ggccgtccac
     4321 gcaggcgata aggatatcgt tcaggccgcg atttttcagt tccgtcagta cattcagcca
     4381 gaactttgca ccttcatttt cggccagcca catacctggc agctctttct gaccatcaag
     4441 gttaataccc agagccagga acaccgcttt gttgataacc cggctgtcct gccgtacttt
     4501 caggacaata cagtcaagat aaataatggg ataaatcgca tccagaggac ggttttgcca
     4561 ttcaaccacc tggtcgatta cggcatcggt gactttcgat atcagcgccg gagagacgtc
     4621 tgcatcgtac atttctttga agactgaggt aatttcgcgg gtagtcatcc ctctggcata
     4681 cagcccaaga tctggttgtc catgccggta atgcgggtct gatttttctt caccagttgt
     4741 ggttcgaagg aaccatcacg atcacgtgga gtacgtaatt ccatcggccc gtcggtagtg
     4801 gtgacggttt tcgtggaata gccattacgg gagttggttc ccgatttggg ctgatttttt
     4861 tcatgtccca gatggtgagt cagttcagcg ttgagagcag cctcaacact gattttcttg
     4921 agcaggcgat cgaactgact gagatcttca ggggttttga gatttttggc cagttcgtta
     4981 gccagagcct gcaattgttt gtcgtccata atttaacctt catttgatgt tgaattgaac
     5041 atatcaaaat caggcaatta cacaaatcta tgtacaggct caaagttatc gccttatgta
     5101 actgctcttg aaagaatcaa tgaacaaatt gagagccagt ttttccggcc tacggccact
     5161 ccccgtaagg tgttctctgc aatgttgttg tcgatttcca cccagccatt actgcagtag
     5221 acgttcaggg cctcccactg tttcagcagg tatgcgaacg cttttgccgt atccgagtga
     5281 cgcgacagtg ttttcatctg agtctgtatc cagtcataca gtgactgcat cagcggcgca
     5341 gctctggctt ttcttgccgc cagatgctgt tctgctgaac atccccggac ttctgcctcg
     5401 atgacataca gttcaccgat acgctgcagg gcttccgtgg tgatgtcggt tggcactctt
     5461 gcatgcacat cgtggatttt tctccgggca tgagccatac acgcggcttc cgttattctg
     5521 ccggattcgt ataacacccg gtaaccaccg taagcatcgg cctgaaacac accgctgtag
     5581 ccggccagat gattttgtgg atggatacct ttccggtccg gtgagtacgc gaaccagacc
     5641 gctgggggca tctgtgaacc ggcattacgg tcatcacgga cgtaggccca cagccgggca
     5701 gtccttgttt taccactgcc cggctcctgt accggaacgg ggatatcatc ggcatggact
     5761 ttaccgggca ccagcacata ctggcgcagg acgtcataca acggctccag cagttcagca
     5821 acagcacctg tccagcgccc cagtgtggca cggctcagct ccactccctg acgacgatat
     5881 atttctgact ggcggtataa cggcagatgg tctgcatatt ttccagcgac aacatgggcc
     5941 agaagccccg ctccggcata actgcgtgct cagggttacc actttaacgt aaaaaccgtg
     6001 tcggcaagcc tgcaccgtca ggggctgagg gcaaaggcct cccggaagtt cagtccggtc
     6061 agttaccgcg cacatggcct gcctgtgtca gaaaatctgt tggagcagga tttttacgcc
     6121 agtggtccga accagaagtg ggcgggagca tcacgtactt acgtacgtga tgaaggctgg
     6181 ctgtatctgg cagtggtcat tgacctgtgg tcacgtgctg ttattggctg gtccatgtcg
     6241 ccacgcatga cggcgcaact ggcctgcgat gccctgcaga tggcgctgtg gtggcgtaag
     6301 agacccagaa acgttatcgt tcactcggac cgcggaagcc agtactgttc agcagattat
     6361 caggcgctgc tgaagcggca taatctgcgt ggcagtatga gcgcaaaggg ctgctgctac
     6421 gataatgcct gcgtggaaag cttctttcat tcactgaaag tggaatgtct ccatggagaa
     6481 cactttatca gccgggaaat aatgcgggca acggtgttta attatatcga atgtgattac
     6541 aatcgctggc gacggcacag ttggtgtggc ggtctcagtc cggaacaatt tgaaaacaag
     6601 aacctcgctt agagcagtgt ccatattacg aggggaggat cagttgttct gtttgagttc
     6661 tatttttgaa tcgcacactg gcaaataatc cataactagg taaaatccaa aaataagtcg
     6721 cgtaaccggg aatagagtta tcttgtattt ggttcataac tacatcgggc gcagtaacac
     6781 ttgaatgcct cataacagaa gcaactcccg cttggttgat ggaacttcat tccaaatagt
     6841 aactagccaa taatcgcctg acctattgat attaaataag taagtaccta aataatcact
     6901 atctgcttgt ggttcataaa gttttgtgtt ttctatactc ttatccgtac tccaaaagtg
     6961 aagatctgtc aggagctcct gaagtgagcc aaaaacagga gtattattac cccataagta
     7021 gtatccacat tcatgtactc gataaaaact aagtttaacc gattcgctca ttggtcagaa
     7081 gttcccttac cttattattc tcaatgggca taattttaca ttgtgcaata gttgctggcg
     7141 agttaaatgg tcacattttc tcccatacgt acatttatgg cttcagttaa agcccgctca
     7201 cgcactgaaa accttgagtg ccctacaggg ctttagggca ctcaatactg ctgctatcag
     7261 cgcaacattt ttctaatatt ttgatgcgat tgtggcagat ttgaacgaag aagatattca
     7321 ttaaaggcta tatgagcatt agtatgcttt gacttatata cgtcgtgttg aaagtcgggt
     7381 cgatcaataa gaccaggata acttcccact gcaatagaac ggtcattcaa actcacaatc
     7441 ataggtgcct cctgagttcc cttaattatg cattgaaaac catccaattc cagacttgcc
     7501 ctcaattgag acaatttttc tacctgaata tcaggtgaag cacaacgagg aaccattccg
     7561 tatagtgcat aacgaagtaa agatgcacct agtttcctat cgagacggct ttgtatatgc
     7621 tggttgtgga aatgattgag acaatctgta caggaggtat cgcactcaca gctttctaac
     7681 aatgcgagtg ttgcagtaag aatgtcatct agattcgctg ctgctacttc cgcataaccc
     7741 gcaccgccgg ataaagtatc ataaaggaag agatccaatg cctgagtatc ttcctctata
     7801 gtgggtaaaa ttctgaaacc agagccaaac tcagcaggat caagatccag ttgtttatgg
     7861 cgactagctg caagccttag tgcttccgct attgtatata atgcatcttc atacatccgt
     7921 aaaacgatag catttgaagt atcagttaca agcggagacc caacggttat tcgtaataaa
     7981 agcaaatcag tacggaaatc atgtccgaga aaaacgcgtt tatactcgcc agagcataag
     8041 cgaggcgttt ccttagttgc tatatattta tacggtcttt catgtgcccc ctttgccggt
     8101 gagtaggaat cataaacgga ggccgcacca cattcgcaac atactgaaaa accactggac
     8161 tccccctcat ttttccctcg gttcaccgtc accagtttct gatctattgc gtgagtaaaa
     8221 acaatatgag cacctccatt attgaactta aaatcttcag gatcaacagg ttgtggatat
     8281 tgtgccgctg ttacataggt gatttcttgc tctctgtcgt cctcattaag ttccttggca
     8341 ttttcaggtc caaagacttc gggctgaatc attatttcta cttttagaat gccaccacag
     8401 atcggacaag tattttcgct attctgatta ttatgaggat cgcggacaaa agagcactta
     8461 tcgcaatgaa taaacttttt gggattattg aaaagctttc ttgcccggtt tagttcgcct
     8521 ttcaatgcat tagaaaaaac accaccagag cgataggttt tcctatcaat aacaatcaaa
     8581 cgtcccgggg catattcact cagagcctga gaaattgatt gctgaggctg ttgtactgtt
     8641 cgcacctcaa aagaacctct aatattcttt acaatttttt ctaccaagaa actacagagg
     8701 cttgtaggaa atgcataact tggtaataaa ccatggtaaa acaggaactc aagtaattct
     8761 tcttgtgcaa atttcattcc accagaagtc tgctcatcat cttcattttc ctcatcaaca
     8821 agaacggcag tttgtggaac aatttcagcc agcccatgga gtgtatttaa aaattcctct
     8881 gctaccttag caaaccagtc actggcagaa agccctccag tttcaagaac aggaggaagc
     8941 catgctgcaa cacttgttct caaatcacca ttagtagata gaatacggtt tttaacccaa
     9001 ttattaaagc tatcgagatt taggccagta tcttttgctc catgaaaaaa atctcgtgta
     9061 gtaccaagtg ctttctcaag tatggcagtt ttctctgcgg gattataaat tccttgttcc
     9121 attaactcgt gaaaaaaggt ctgaactaaa aaagaatgaa cgtgtcttct ggctattttg
     9181 ggattattta ctttcacttc aggtgtacga ggagaacctg caacaatgcg ttcaggatta
     9241 aggaaataat aactatcatg agggccattt tgagaatatg taaccaccgt tgaaacagat
     9301 gcgccacggc ggcctgctcg cccagcacgt tgctgataat tttctcgttg cggagggacg
     9361 tttcttaaag caacagcaac cagagatcca atatcaaccc ccacttccat cgtcgtcgta
     9421 caactaagta catcaatggg cttgtcgtta tcattaataa gaacatcttg gaatctcagt
     9481 tcgtagagtt ctgtagtggc atgaacgctg gccctatctc tatgtgagag ttgagcagta
     9541 tgctcttcaa cgctaaggtt tagaagccgc gaatttgaaa ccaaaacttc ttctaccggc
     9601 gaacgccaga atccttttcg tgcattaata tagctgcttt ccgacggctc gactgttttg
     9661 acactgttac taccacaagc aaggcaagta gaatgttcta aagcaaatgg cattagtgct
     9721 gtgcagtcat tacattgttt ccagacatgc aagagatcta ttttcagacg tagtgcattt
     9781 ggagcaagaa agtagacacc atcttttcct aatgtcagat gagtacgaaa aatctcctcc
     9841 aaaacttcca cataaagctc ccccatagca ggatactgta tcagggtttt cctaagagct
     9901 tttccaaacc gtccttgact accccaagtg ggtttgtaga atccagctgc tttgattcgt
     9961 agtgtcgaat caatagattc atcaaaagca aattcagtga gtaaggtatc aatccaagca
    10021 acagctaaag catgaacatc cttgctctca atattgagtt tcttggactg cacatcttcc
    10081 cacatttttt ttgatttaag ctgcgatggt tcaacaaaac caactgttgt tccggaaaga
    10141 gaatagtaat tgctacataa aagtttaagc aacgctattt tatatcgtga cggtgactct
    10201 tgggggctga agctatcatc aaaagcttga gccagatcgc tattataatc acgataaaat
    10261 tcatcacggg ccattacaac ttttcgtgaa tcttccccat caaaaataag caagtcatgt
    10321 tcagaaagga ctgataggaa agcaaggtaa agtactgatg ttggtttggg ttcccgattg
    10381 atatctttca gtttagaaca ggcgagagca atggattgcc gaaacaaatc aagctcaata
    10441 tctctaggaa tatcacgtgc aagccgagct gctttttgtc ggccatcaga aaaaataagt
    10501 actttttttc ccccattggg atgtttacca tcaataggac gactcgctgg ctggtgagat
    10561 atctgtgtac gtactaacgt tgtaaaaggt gcttcccctt ttgtaacatg atccataatt
    10621 ttagacggtt cattctgagc acttcttgtc ttacgcatac aaacagggca ttcatcaaat
    10681 gtaatttctg atccagactt aactcggtca ggtataaaga ctttacgata accagaatcc
    10741 tcatcttgac actgtttaga aaggcgtcct gttgctatat gtagccatct gtcctggtaa
    10801 tcactatgta catgaggtgt ttcctcgacc aatatatcta tgggaacaag atcgatatcc
    10861 tcatcttctg ataatggtcc gttcggctgg tgccatacaa agtccatttc ggaactaacg
    10921 tatccacgaa taaaagccgc accgcagtca cggtgggtaa ataattcgta gactcgccct
    10981 tttgaagcgc atttacattg atccagtggt ttcgtataaa ggcggccaag tatagttggc
    11041 ccgctatggt tacccaaacg ttgattacaa tcgggatcta tacaggcata aagacctggt
    11101 aatccccgat aaaaaagatg catgcgagtt ggaataagca ctctgccatc ggatgccctc
    11161 tgagcatagc aacccaatgc gagtaatgca tctgttgctc gctctgcgat ttgctgtgga
    11221 gagtctggaa aaaggttttc actcaagata tttagcttaa ccgctttacc tgaaactatt
    11281 tcaataagcg tttcaatagg accaaaacca gtcaaattat caaataacca attacgtagt
    11341 gtactatgat cttttatcat cggcttttgc cagcccattc gttcggcaag agactctatt
    11401 gctgcatatg cagattcaag atcctcagct acgcactgaa atgaatttag gtcgaattca
    11461 gccagtgcat tagcttcttt actggtaaca atttgtgact caggacgcga ttcccttgta
    11521 ccctcaataa ttcgaaattt cctcgaagag gttggtgata agccagttaa gtcttgggca
    11581 aaacgttctc catcctcaat ggaccctaga ctagcactgg taaggatgca gcgcatacgt
    11641 tcccggggaa tatccaaccg agcacagagg cgacgtatta aaagggctac ctctgctccc
    11701 cctgctcctc tatacatatg cgcttcatca agcactaaga taagctcatt catctcatca
    11761 gctttgagcc attccttagt ctgctcaaaa atattacgct cgataggtcg catcagcata
    11821 tactcaagca tggagtagtt cgttatcagt aattctggac agcgattctg tatttcatgc
    11881 cgggtcatta gctcacgatc ctcaggctgg gtaattagcc tctcccccca attgttcaaa
    11941 acaaattgct tacccgtttt tttgcctgag acgtaggttt tagcctgaga tgcgctttgc
    12001 ccataaaaag catcaagatc tttacttggc cagcgaccaa tgcggttcag ttccgcacgt
    12061 acgggggcgt tatttgcgag tttattgtaa aactcatcga aaaggggttt gataaaaagc
    12121 tcgtctcgtc tagagctacg acgaccaggg taaggcgttc ttcccgtata agcgccaaag
    12181 cgtacagggg cacatcttcc agatctcagt attttagagg cttcagaatc accaaaaaga
    12241 cgtctgatac gagcaagttg atcgttaact aatgcattca ttggatataa taaaattgct
    12301 ctacaacctg gaagggatgc agatttaggt ctctcggaag attcaatcgc caattttcca
    12361 ataattggca ttagaaagct ttcagtctta ccggagcctg tacctgttgc aatgaccaga
    12421 tcagaagcgt tttcgccaag aaatgactca agtgcctgtg attggtgttt ataggggcgt
    12481 tgatagaggc caattccaag ttctgatagt tgagttagca cattacttgc tgcttcggga
    12541 ataggcaatt cactgtatgg cgcaccaggt tcataaattg gggttgcttc tatataagga
    12601 gcttgagcaa tagtttcatt ttgctgtaaa agcgctcgtc gctcagcaat taacccttca
    12661 tcccggatat gatattgtgc ctcaatgtat tttctcagat tttcactaag gcttttcacg
    12721 gtatcatgta tacctgaacg ttcagttact ttatttttat taatcatatt tcattccgtc
    12781 gcgtgaattc gttaatccaa ataataccaa gcccttcaaa agcgctacgc actatgggta
    12841 atatttcctc ggggaataca tggtgtgtta ttcctgaatg ttcattttca aaacctgatt
    12901 ctctccagcc tagcaggagt accttcgttt ctcgtcttgg taatcttctg gttaatctta
    12961 atcttgctag cccattagat attttgatac ggacctttag cggacaatta tcttttgtat
    13021 cgagaaaaaa acgtaatctt ctgacatcat cagacgattc taacgaattc attctacata
    13081 agcgtccagc ttcaatttct cctaaataat aggaaaagtg attatcaacg gacattctgc
    13141 ataagtagat ttgcttttta ttaaaagaaa gttctgacaa atggagccat tttccccgca
    13201 catatgcagt cgtttcactc atattgccag gtgcatcgat aaagccggaa atagttccag
    13261 atagtagtct ggatgaccat tcttcattgc cttctgctgg ggcaccaatc cactcatttg
    13321 catcccagat ttcaacactt ccagtacaca ctttttcttc aaccaagcga actcttccag
    13381 cagatttagc taccacgccc gttgaaaatg tacaggaagg ctctccacca aaaaaaacag
    13441 ccatcttatt gtcaattcgt actgcatgtg ggggagctgt tagccaatta ccaccttcta
    13501 acttaatcat gtcacccaat tcagccaagc tgttcaatat agacataatg cccggatgta
    13561 tagcattatc aatatcagca atgtctggcc ataaaggtac caattttcgc cttactgcac
    13621 tagtgagtcg aagcgaggtt acagtttgcc tagcttcttc tgtttcagat atagaatatt
    13681 gatataatac gctccttaag cattctgaaa ctgctgaaat tctcaaccct tcatcatcaa
    13741 atcgacaatt taatgcagtt gataacatat ccagtgcttt gtctcgtgat actgctctcc
    13801 actccattaa ccacctcctc aagaagatat ttttgcacta taatttttta attcacgatt
    13861 ggcaatatct tctatctttt ggacggggcc acctttaatt tttgcccatg taagagtata
    13921 aaaagacaag acagaataaa gatcataaat ataatttcca ttagggatcc ttatgagtga
    13981 agtatagaaa atatgaatca ttctcttcca caaagttccc ccatcaaatc cagcttcgtc
    14041 cagtaatgct cttaattcat ctacataatc atcaacacta tcaagatgta gtccatcaat
    14101 atttgtccat ttatccttgg caagacaacc tggtttcaat tggggtaaag tagctgataa
    14161 accacgcatt tttaatgtat cagtatcaat aacaggtccc aactcggcca acattcctcc
    14221 atcagggaat gtagctgggc cttgtttcca ggtagctatc aatgccagat ggtcataatt
    14281 tgttggatgt atttgccgtt gaacaactat atctctaatt gccgctccat aaatctcaaa
    14341 tgctgaaaga ttggcccctt tcaaaaggag acagcgagat gattcagcta tagtctctat
    14401 aaaatcaaaa gcatccatgt cagaaataat accaactgga actctccata tgtgataacg
    14461 tggtgcacca atagcaatgg caattgcatc cgcgataata tctgccaaag agccactgaa
    14521 ttgaatgatt tgtccagaaa cgaaggtagc taatgtcaat ctagccaatt tttctgctga
    14581 attttttgtc aatccaacag cctgtaaatt taaagaaatt ctattagata tgtcttcgga
    14641 ggatgaaaga tactcataat tttcatagtg cgctatttca tggaattttg taaatctatt
    14701 ggtgacactc acttcg
//
LOCUS       DAPHDW010000111        14548 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.112, whole genome shotgun sequence.
ACCESSION   DAPHDW010000111 DAPHDW010000000
VERSION     DAPHDW010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.112"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(140..892)
                     /gene="yecC"
                     /locus_tag="RH057_003326"
     CDS             complement(140..892)
                     /gene="yecC"
                     /locus_tag="RH057_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HDV3129649.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKFLLQ"
     gene            complement(889..1557)
                     /gene="tcyL"
                     /locus_tag="RH057_003327"
     CDS             complement(889..1557)
                     /gene="tcyL"
                     /locus_tag="RH057_003327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HDV3129650.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(1572..2558)
                     /gene="dcyD"
                     /locus_tag="RH057_003328"
     CDS             complement(1572..2558)
                     /gene="dcyD"
                     /locus_tag="RH057_003328"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HDV3129651.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDDVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELTTRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(2663..3463)
                     /gene="tcyJ"
                     /locus_tag="RH057_003329"
     CDS             complement(2663..3463)
                     /gene="tcyJ"
                     /locus_tag="RH057_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3129652.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKERGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(3551..4102)
                     /gene="fliZ"
                     /locus_tag="RH057_003330"
     CDS             complement(3551..4102)
                     /gene="fliZ"
                     /locus_tag="RH057_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HDV3129653.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDALLDENGWQTEQKSWAALCRFCGDLHCKTQSDFFDIIGFKQFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(4148..4867)
                     /gene="fliA"
                     /locus_tag="RH057_003331"
     CDS             complement(4148..4867)
                     /gene="fliA"
                     /locus_tag="RH057_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
                     /protein_id="HDV3129654.1"
                     /translation="MNSLYTAEGVMDKHSLWQRYVPLVRHEALRLQVRLPASVELDDL
                     LQAGGIGLLNAVERYDALQGTAFTTYAVQRIRGAMLDELRSRDWVPRSVRRNAREVAQ
                     AIGQLEQELGRNATETEVAERLGIDIADYRQMLLDTNNSQLFSYDEWREEHGDSIELV
                     TDDHQRENPLQQLLDNNLRQRVMEAIETLPEREKLVLILYYQEELNLKEIGAVLEVGE
                     SRVSQLHSQAIKRLRTKLGKL"
     gene            complement(5032..6507)
                     /locus_tag="RH057_003332"
     CDS             complement(5032..6507)
                     /locus_tag="RH057_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HDV3129655.1"
                     /translation="MAQVINTNSLSLLTQNNLNKSQSSLSSAIERLSSGLRINSAKDD
                     AAGQAIANRFTANIKGLTQASRNANDGISVAQTTEGALNEINNNLQRIRELSVQATNG
                     TNSDSDLSSIQAEITQRLEEIDRVSEQTQFNGVKVLAENNEMKIQVGANDGETITINL
                     AKIDAKTLGLDGFNIDGAQKATGSDLISKFKATGTDNYQINGTDNYTVNVDSGVVQDK
                     DGKQVYVSAADGSLTTSSDTQFKIDATKLAVAAKDLAQGNKIVYEGIEFTNTGTGAIT
                     ATGNGKLTANVDGKAVEFTISGSTDTSGTSATVAPTTALYKNSAGQLTATKVENKAAT
                     LSDLDLNAAKKTGSTLVVNGATYDVSADGKTITETASGNNKVMYLSKSEGGSPILVNE
                     DAAKSLQSTTNPLETIDKALAKVDNLRSDLGAVQNRFDSAITNLGNTVNNLSSARSRI
                     EDADYATEVSNMSRAQILQQAGTSVLAQANQTTQNVLSLLR"
     gene            6762..8174
                     /gene="fliD"
                     /locus_tag="RH057_003333"
     CDS             6762..8174
                     /gene="fliD"
                     /locus_tag="RH057_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001616262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HDV3129656.1"
                     /translation="MASFTSLGVGSNLPLDTLLNNLTIAEKKRLNPITQQQSDNTARL
                     TAYGTLKSALEKFQTANTALNKADLFKSTTVTSSNEDLKVSTEAGAAPGIYTISVTQL
                     AQAQSLRTDSPIIIASTKDALGDESSDTRTIKITQDGRKEPLEIKLNKDQTSLDEISK
                     AINDADSGISASIVKVKDGDYQLVLTASEGLANKMTISVEGDSKLNDLLAYDSKTNTG
                     NMKELVNAQNAQLNVNGIDIERSSNKITDAPQGVTLDLTKKVTDVRVTVTKSNDKATE
                     AIKGWVDSYNSLIDTFNTLTKYKEVDPGAEAQDKNNGALLGDSVVRTIQSGIRAQFAN
                     GASDGAFKTLNEIGIKQDGTTGKLKIDDDKLKKVLNENTASVRELLVGDGKETGITTK
                     IATEVKGYLADDGIIDSAQDSINATLKKLTKQYLSVSASIDDTVARYTAQFTQLDTMM
                     SKLNNTSTYLSQQFTAMSNS"
     gene            8189..8599
                     /gene="fliS"
                     /locus_tag="RH057_003334"
     CDS             8189..8599
                     /gene="fliS"
                     /locus_tag="RH057_003334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HDV3129657.1"
                     /translation="MYTASGSKAYAQIGVESAVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSLIQDPV"
     gene            8599..8964
                     /gene="fliT"
                     /locus_tag="RH057_003335"
     CDS             8599..8964
                     /gene="fliT"
                     /locus_tag="RH057_003335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HDV3129658.1"
                     /translation="MNHAPHLYFAWQQLVEKSQLMLRLATEEQWEELIASEMAYVNAV
                     QEIAHLTEEIDPSTTMQEQLRPMLRLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            9042..10529
                     /gene="amyA"
                     /locus_tag="RH057_003336"
     CDS             9042..10529
                     /gene="amyA"
                     /locus_tag="RH057_003336"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HDV3129659.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIENPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAEYWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVESWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(10563..10976)
                     /locus_tag="RH057_003337"
     CDS             complement(10563..10976)
                     /locus_tag="RH057_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310694.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HDV3129660.1"
                     /translation="MKKLAIASALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRVIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            11163..12368
                     /gene="yedE"
                     /locus_tag="RH057_003338"
     CDS             11163..12368
                     /gene="yedE"
                     /locus_tag="RH057_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HDV3129661.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPSSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFTALMLIIGWEKRFFRRA
                     APQTAKEIA"
     gene            12365..12598
                     /gene="yedF"
                     /locus_tag="RH057_003339"
     CDS             12365..12598
                     /gene="yedF"
                     /locus_tag="RH057_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HDV3129662.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVMSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            12707..13378
                     /locus_tag="RH057_003340"
     CDS             12707..13378
                     /locus_tag="RH057_003340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HDV3129663.1"
                     /translation="MCGRFDQSQTRVDYLALLAEDIERDIPYDPEPIGRYNVAPGTKV
                     LLLSERDEHLHLDPVFWGYAPGWWDKPPLINARVETAATSRMFKPLWQHGRAICFADG
                     WFEWKKEGDKKQPYFIYRADGQPVFMAAIGSTPFERGDEAEGFLIVTAAADQGLVDIH
                     DRRPLVLSPEAAQEWMRQEIGGKEASEIATNGCVPANQFTWHPVSRAVGNVKNQGAEL
                     IQPVC"
     gene            complement(13418..>14548)
                     /locus_tag="RH057_003341"
     CDS             complement(13418..>14548)
                     /locus_tag="RH057_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3129664.1"
                     /translation="ACRFVFNRALARQNENHEAGNKYIPYGKMASWLVEWKNATETQW
                     LKDSPSQPLQQSLKDLERAYKNFFQNRAAFPRFKKRGQNDVFRYPQGVKLDQENSRIF
                     LPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMVGLDAGV
                     AKLATLSDGTVFEPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSCIAN
                     IRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILDQ
                     GWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYTA
                     NADVNGARNILAAGHAVLACGEMVQSGRPLKQEPTEMIQATA"
BASE COUNT         3582 a         3668 c         3574 g         3724 t
ORIGIN      
        1 tcaggcaatg aattactttt gcaagccatc gcattctctt atgttattaa tgagttatgc
       61 tgatttgtta agcagtttta tcacgcttga aatggcgtcc cgccccgaca ggtgaatcgt
      121 cggggctgat tttttcttat tattgcagca gaaacttctc gaggaactgg cgggtgcgag
      181 gctgctgggg atcggcaaat aacgcttttg ctgccccctg ctcgactatc cgcccctggt
      241 ccataaaaat cgcccggtcc gcaacatccc gggcaaagct catttcgtgc gtcacaatca
      301 ccatcgtgcg cttttcctgc gccagctggc ggattgtgtt caggacttcg cccaccagct
      361 ctggatccag cgctgacgtt ggctcgtcaa acaaaatcac ctcaggacgc attgccagcg
      421 cacgcgcaat cgcaacacgc tgctgttgac cgccagacaa acgacgtgga tagctggttt
      481 ctttaccggc cagcccaact ttcgccagca gctcgcgagc gcgcgccgtg gcctcttctt
      541 tcggttcgcc tttgacgatc accggccctt caataatgtt ttccagcacc gtacgatgcg
      601 gaaacaaatt aaagttctgg aagacaaacc cgacgtgctg acgcaactgg cgaatcagcg
      661 atttttgctg acttaatgaa cgtgcagtat caatagtgat atcgccaacg gtgatcgttc
      721 ccgcttccgg ttgttccagc agatttatgc tgcgtagcaa cgtggttttg ccggaaccac
      781 tcggaccgat aattgccacc acttcgccag gctttacctc gaggtcgata ccgtgcagca
      841 ccgtctgacc gtgaaatttt ttcaccaggt tcttaacttc aatggcactc attttggttc
      901 tctctcctgg cgattaagtt gattctcaaa atggttctgc aacgtcgata atactgtcgc
      961 catgatccag tagatgagcg aagccgccag gtacatggtg aacacctcca gcgtgcgcga
     1021 ggtaatcaac tgcgcttgac ggaacagctc cggcacctgg attgtcgcgg ccagcgacgt
     1081 atcttttacc aggctgataa agctgttcga cagcggcggc aacgccaccc gcgccgcctg
     1141 tggcaaaatc gcgcgacgca tggtctgcca cggcgtcata ccaatactgg cagcggcttc
     1201 ccactgacct ttatcaatag aagaaatggc ggcgcgcagc gtttcggcag cataggcggc
     1261 agtgttcagc gacagaccaa tcattgctga cggaatggga tctaattcga taccaaactg
     1321 cggcaggccg taatagatca taaacagttg tgcgatgagc ggtgtaccac gaaaaataga
     1381 gatataaaaa cgcgccagcc agcgcacggg ccagattggc gacagacgca tcagcgcgag
     1441 aataaaccct agcagtaagc caaaaaacat gccgccgata ctgagttgca gggtataccc
     1501 agcccctttc aacaggaacg gcaaggaatc aataaccagt tgtatacttt cttgcatgag
     1561 cgttcccgga gctaaacgtg gggatgatag gcgaacagcg caggcgcgcc gccggtatga
     1621 ataaacagaa tcggcccttc atctttgaag cgtttctgac tgataccgtc aatcagcccc
     1681 gccatcgctt ttccggtata cacaggatca agcagaatgc cttccagacg cgccagcaat
     1741 ttcactgctt ccatgccttc gtcgttcggc acgccgtagc caggtgcaaa atagtcatcc
     1801 cagagtaaaa tttccgctga tgcggtcagc tccagttctt tcgcaatcgc ctgttgtagg
     1861 ttaaccactt tcggcaattg atcggcaacg gaacgcgaca cggtcacgcc aatcagttcg
     1921 ctttcaggca tcaggtgttc cagcccaaca gccaatccgg cgtgagttcc ggcactgccc
     1981 gatgcgacca ccaccgacga aatattaacc gccccttcac actgttgcgc gatttccagc
     2041 gcactctcca cataacccag cgcgcccaga gcattagaac cgccaaccgg aatgacatac
     2101 gggcgaaagc cttgtgcttc gactcgcgtc gtcagctctt ccagttgggc attgggatcg
     2161 gtcagtgcgt cgcacatttc aatctgggta ttgaacagat ccagcaacaa acgattgccg
     2221 ttggttaaat agttttctgc ggttgtgcca ataggatttt ccagcagcgc cacgcagtgc
     2281 agaccgagtt tcgccgcgac tgcggcagtc tggcgcacat ggttagactg gatcgcgccg
     2341 gcagtaatca gcgtatcggc accttcgcgc agggcatctg ccgcgagaaa ttccagctta
     2401 cgtaatttat tgccgcccat tgccatgggt gtgacgtcat cccgtttgat gaaaatttcc
     2461 cgtccaagat aatcagaaaa gcgcggcaga tattcgagcg gcgttggcgc gccgataaac
     2521 tccaaccgtg gaaaacgggt taaattatgc agtggcataa cagcctccga tgtgtgttgt
     2581 tgtgattttc ttattatgca cgctgaaaac gcgtaaataa aaaaggcgct agtgaaagcg
     2641 cccttttttg tcattatgct gattatttgg tcacatcagc accaaaccat ttttcggaaa
     2701 gagcttgcag agtgccgtct ttttgcattt ccgcaattgc atcattcact gctttcagca
     2761 ggtcttcatt tcctttacgc agcgccacgc cagactcctg acgggagaat gcttcaccgg
     2821 ttactgccag cgtatcgttg gttttcttta ccagatccag cgctgccaga cggtcaacga
     2881 ggatcgcatc gatacggcct acgcgcagat cctgatattt ggtcgggtca tcatcatagg
     2941 tacgcacatc gacgccctga acattctgcc gcagccactc ttcatagttg gtgcccagac
     3001 cgacacccac ttttttgcct ttcagatcag cagccgtttt aatggtgcct tcgttacctt
     3061 ttttcaccag cgcctgaata ccggaaatgg tgtaaggggt agagaaatcg tattttttct
     3121 tgcgctcgtc agaaatggtg acctgattaa tcaccacatc aatgcgttta gagtccagcg
     3181 acgccagcat accgtcccat ttggtcggtt ttagtgacgc ctcaacgcca agatgttttg
     3241 ccagctgttg ggcaaattcc acttcaaaac cggttaattt gccgtcatcc ccctgaaaac
     3301 tgaacggcgg ataagtgcct tccagcccta ccagcagcgt gccgcgctct ttgactttat
     3361 taagcagacc ttcatctgca aaacttttaa cgctcatgcc cgcaaccagc gccacggcca
     3421 tcacacccat caatgcctga cgtcccagat gtgctaattt catattcacc ccgaatgttg
     3481 ttatgtctgt ttgcagtgta gagccattgt ttgtaaacac aaaaacaact ccgctacatc
     3541 ttattcttat ttaatatata tcagaagcag gcaggctgga ggatttctgc acaagtccgg
     3601 tacaggtttg ccgctgatag tgttgatact tccgtaatgc gatgcggtaa ttgttggtgc
     3661 tggtggtggg cagccacggg gcgaggattt catcaaggaa accgtcctgc agctgatcga
     3721 gggaaatatt ttgctcgtgt agatgattcc ccaaacggcg cagacgaacg acatattcac
     3781 gcaccgtacc agggctcatt tcagtttgct caaaaagaaa ttgcttaaag ccgataatat
     3841 cgaaaaaatc actctgcgtt ttgcaatgta aatcaccgca aaatcggcac aatgccgccc
     3901 atgatttttg ttccgtttgc cagccatttt catcaagcag cgcgtccagg cgggaaatct
     3961 cgattttatt cactattttg ccgtcgcgaa cgagggtaat gcgatcgagt aatttacggc
     4021 aatgcgcgca atgagtctgg ctgtgtttaa agtctttaag atagcggctt aatggccgtc
     4081 ttttcaggtg ctgcaccatc attaagaact cctggtagtc aaaattaaag tgcggcattt
     4141 actgacgtta taacttaccc agtttagtgc gtaaccgttt aatggcctgg ctgtgtaact
     4201 gactgacccg cgattccccg acctccagca ccgcgccaat ctctttgaga ttcagctctt
     4261 cctggtaata gaggattaat accagttttt cgcgctccgg caacgtttcg atggcttcca
     4321 tcacccgctg gcgcagatta ttgtccagca gttgttgtag cgggttttct cgctgatgat
     4381 catcagtaac cagttcgatg ctatcgccgt gctcttcgcg ccactcatcg taggagaaga
     4441 gctggctgtt attggtgtcg agcaacattt ggcgataatc ggcaatatcg atccctaaac
     4501 gttccgctac ctcagtttcc gtggcgttgc ggccaagttc ctgctccagt tgccctattg
     4561 cctgtgccac ttcacgcgcg ttgcgtcgca cgctgcgcgg cacccagtca cggctgcgaa
     4621 gttcatccag catagcgcca cggatacgct gcactgcgta agttgtaaat gccgttcctt
     4681 gtagggcgtc atagcgttcg acggcattaa gtaacccaat gccgcccgcc tgtagcagat
     4741 cgtcaagttc cacgctcgcg ggcagtcgaa cctgcaggcg caatgcttcg tgacgcacca
     4801 gcgggacata acgctgccac agcgagtgtt tatccattac accttcagcg gtatagagtg
     4861 aattcacgat aaacagccct gcgttatatg agttatcggc atgattatcc gtttctgcag
     4921 ggtttttaat cggacgatta gtgggtgaaa tgaggggtta tttgggggtt accggtaaat
     4981 tgcgggcaga aaaaaccccg ccggtggcgg ggtttgagcg ataagtgtaa attaacgcag
     5041 cagagacagt acgttctgcg tggtctggtt agcctgtgcc agaacagagg tacccgcttg
     5101 ttgcaggatc tgcgcacgag acatgttaga cacttcggtc gcgtagtcag catcttcgat
     5161 acggctacgg gcagaagaca ggttgtttac ggtgttgcca aggttggtga tggcagagtc
     5221 gaaacggttt tgtactgcac cgaggtcaga acgcagattg tcaactttag ccaatgcttt
     5281 gtcgatagtt tcgagcgggt tggtggtaga ttgcaacgat tttgctgcat cttcgtttac
     5341 cagaatcggg ctaccacctt ctgatttgct cagatacatg actttattgt taccagaagc
     5401 agtctccgtt atcgttttac catctgcact aacatcgtaa gttgcaccgt taacaactaa
     5461 cgtgcttcct gttttcttgg cagcgttcag atcaagatca gatagtgtcg ctgctttatt
     5521 ttcaactttt gttgcagtca attgccctgc actatttttg tatagggctg tcgtaggggc
     5581 aacggttgca ctagtacctg atgtatcagt actccccgaa atagtgaatt caacagcctt
     5641 accatcaaca ttggcggtta atttaccatt acctgtggca gttatagcgc cagtgccggt
     5701 atttgtaaat tcgatacctt cgtagacaat cttattacct tgagctaaat ctttagcagc
     5761 cactgcaagc ttagttgcat caatcttgaa ttgagtatca ctgctggtcg taagtgaacc
     5821 atccgcagca ctcacataaa cttgtttgcc atctttatcc tgtactactc cactatctac
     5881 attaacagta tagttatcag taccgttaat ttgataatta tcagtacctg tcgctttaaa
     5941 tttagaaatc aggtcactgc cggttgcttt ctgcgcgcca tcgatattaa aaccgtccag
     6001 gccgagagtt ttcgcatcaa tttttgccag attgatagtg atggtttcac catcattagc
     6061 accaacctga attttcattt cattattttc agcaaggact ttcacgccgt taaactgagt
     6121 ttgctcagat acacggtcaa tttcttccag acgttgagta atttcagcct ggatagaaga
     6181 aagatcgctg tcagagttag taccgttagt tgcctgaaca gaaagttcac gaatacgctg
     6241 caggttgttg ttaatttcat tcagcgcacc ttcagtggtc tgcgcaacag aaataccatc
     6301 attcgcgtta cgggaagcct gggtcagacc tttaatattt gccgtaaaac ggttagcaat
     6361 cgcctgacct gctgcatcgt cttttgcgct gttaatacgc agaccagaag acagacgctc
     6421 aatagcagag ctaagagaag actgagattt gttcaggtta ttctgggtca acagcgacag
     6481 gctgtttgta ttaatgactt gtgccatgat cttttcctta tcaattacaa cttgatgtta
     6541 ttgggctgtt gcccacggtt tctcaccgta tcccttgtat cggcacctga atttcgaact
     6601 ttagaaaatt ttagtttctc tcctgttttt tcttaacggc tgaaatatcc gtaatataca
     6661 actctactct gcgcatcatc ttcgtgaatc aactattaaa ctttgcgcac aaattgccga
     6721 taacccgctt atctactgtt tgcaatcaaa aggaattagg tatggcttca tttacatctc
     6781 ttggcgttgg ttcgaatctg ccgctggata cattgctaaa caacctgacc atcgctgaaa
     6841 aaaaacgctt aaacccaatt actcagcaac agagtgacaa taccgcacgt ctgacagcct
     6901 atggcacatt aaaaagtgca ttagaaaaat tccagactgc aaatactgcg ttaaataaag
     6961 ccgatctgtt caaaagcact accgttacca gtagcaatga agaccttaaa gtttccaccg
     7021 aagcaggtgc tgcccccggt atctatacca tcagcgtaac ccaactggcg caggcgcaat
     7081 ctttacgaac cgattccccg ataatcattg catcaacaaa agatgcactg ggtgatgagt
     7141 catcagatac acgcacaata aaaattaccc aagacggccg taaagagccg ctggaaatca
     7201 agctcaataa agatcagacg tcattggatg agatcagcaa agccattaac gatgccgata
     7261 gtggaatttc cgctagtatc gttaaagtca aagatggtga ctatcaattg gtattgaccg
     7321 ccagtgaagg tctggccaat aaaatgacca tatctgttga gggtgacagt aaattaaatg
     7381 atctgctggc ttatgacagc aagaccaaca ccgggaatat gaaggaactg gttaacgcgc
     7441 aaaatgcaca gctaaacgtt aacggtatcg atattgaacg ttcaagtaac aaaattaccg
     7501 atgctcctca gggagtgact ctcgatttga ccaaaaaagt cactgacgtc cgggtcacgg
     7561 tgacaaaaag caacgataaa gcaaccgaag caattaaagg ctgggtggat tcatataact
     7621 ccctcattga cacctttaac accctgacaa aatataaaga agtagaccca ggtgctgaag
     7681 cacaagataa aaataatggt gcacttctcg gagacagcgt tgtacgtaca attcaaagcg
     7741 gcatccgcgc acaatttgca aacggtgcca gcgacggtgc cttcaaaaca ttaaacgaga
     7801 tcgggattaa gcaggacggc acgacgggta agctgaaaat tgatgatgat aaactcaaaa
     7861 aagtcctgaa tgaaaataca gcatccgtac gtgaattgct ggtaggcgat ggtaaagaaa
     7921 caggaattac caccaaaatt gccaccgagg taaaaggtta ccttgccgat gatggcatta
     7981 ttgatagcgc acaggacagt attaacgcca cactgaaaaa actgaccaaa cagtatctat
     8041 ccgtcagtgc cagcattgat gacaccgtgg cgcgttacac ggcacagttt acgcaattag
     8101 ataccatgat gagcaaactg aataacacca gtacctacct cagccaacaa tttactgcaa
     8161 tgagtaattc ctgatcacgg aggtcagcat gtataccgca agcggctcaa aagcctatgc
     8221 acaaattggc gtcgaaagcg ccgtaatgag cgcaagccag cagcagctgg tcaccatgct
     8281 atttgatgga gtgctgagcg cactggttag agcgcgcctg tttatgcagg acaacaatca
     8341 gcaaggcaaa ggcgtctctt tgtcaaaagc gatcaacatc attgagaacg gactgcgggt
     8401 gagtcttgat gaagagagca aagacgaatt aacccaaaac ttgattgctc tttatagcta
     8461 tatggtcagg cgcttgctgc aagccaattt acgcaacgat gtctccgcag tcgaagaagt
     8521 ggaagcatta atgcgtaata ttgccgatgc ctggaaagag tcgttactct ccccttcttt
     8581 gattcaggac ccagtctgat gaaccatgca ccgcatttgt atttcgcctg gcaacaactc
     8641 gtcgaaaaaa gccagctcat gttacgcctg gcaacggaag aacaatggga agaactcatc
     8701 gccagcgaaa tggcgtatgt gaatgcggtg caggagattg cacatctgac tgaagagatt
     8761 gacccgtcca ccacgatgca ggagcagctc cgcccgatgc tgcgcctgat tctcgacaac
     8821 gaaagcaagg taaagcagtt attacagatt cggatggatg aactggcgaa actggtcggt
     8881 cagtcatcgg tgcaaaaatc ggtgttaagt gcctatggcg atcagggcgg ctttgtgctg
     8941 gctccgcagg ataacctctt ttgaatctga acgagtcgat ggctcgcgaa taatccgatt
     9001 acggctacgc ttctaatgtt ccccttgaat ggagtcgaag aatgcgtaat cccacgctgt
     9061 tacaatgttt tcactggtat tacccggaag gcggtaagct ctggcctgaa ctggccgagc
     9121 gcgccgacgg ttttaatgat attggtatca atatggtctg gttgccgccc gcctataaag
     9181 gcgcatcggg cgggtattcg gtcggctacg actcctatga tttatttgat ttaggcgagt
     9241 ttgatcagaa aggcagcatc cctactaaat atggcgataa agcacaactg ctggccgcca
     9301 ttgacgctct gaaacgtaat gacattgcgg tgctgttgga cgtggtagtc aaccacaaaa
     9361 tgggcgcgga tgaaaaagaa gctattcgcg tgcagcgtgt aaatgctgat gaccgtacgc
     9421 aaattgacga agaaatcata gagtgcgaag gctggacgcg ttacaccttc cccgcccgtg
     9481 ccgggcaata ctcgcagttt atctgggatt tcaaatgttt tagcggtatc gaccatatcg
     9541 aaaaccctga tgaagatggc atttttaaaa ttgttaacga ctacaccggt gaaggctgga
     9601 acgatcaggt tgatgatgaa ctcggtaatt tcgattatct gatgggtgag aatatcgatt
     9661 ttcgcaatca tgccgtgacg gaagagatta aatactgggc gcgctgggtg atggaacaaa
     9721 cacaatgcga cggttttcgt cttgatgcgg tcaaacatat tccggcgtgg ttttataaag
     9781 agtggatcga acacgtacag gaagttgcgc caaagccgct gtttattgtg gcagagtact
     9841 ggtcgcatga agtcgataaa ctgcaaacgt atattgatca ggtggaaggc aaaaccatgc
     9901 tgttcgatgc gccgctgcag atgaaattcc atgaagcatc gcgcatgggg cgcgactacg
     9961 acatgacgca gattttcacg ggtacattag tggaagccga tcctttccac gccgtgacgc
    10021 tcgttgccaa tcacgacacc caaccattgc aagccctcga agcgccggtc gaatcgtggt
    10081 ttaaaccgct ggcgtacgcc ctaattttgt tgcgggaaaa tggcgttcct tcggtgttct
    10141 atccggatct ctacggtgca cattacgaag atgtcggtgg tgacgggcaa acctatccga
    10201 tagatatgcc aataatcgaa cagcttgatg agttaattct cgcccgtcag cgtttcgccc
    10261 acggtgtaca gacgttattt ttcgaccatc cgaactgcat tgcctttagc cgcagtggca
    10321 ctgacgaata tcccggctgc gtggtggtca tgtcgaacgg ggatgatggc gaaaaaacca
    10381 ttcatctggg agagaattac ggcaataaaa cctggcgtga ttttctgggg aaccggcaag
    10441 agagcgtagt gaccgacgaa aacggcgaag caaccttctt ttgcaacggc ggcagcgtca
    10501 gcgtgtgggt tatcgaagag gtgatttaaa ttcatccccg gcggcaagcc ggggagattt
    10561 cattacggca gtggcgtcgg taacggcgct ttatccagcg cggcggcaca ctctgccgtc
    10621 gggcgatcca ctcgctccat cgtcatacca tcgtactcaa tggtgttacc gtcacgctca
    10681 acctcgtaca gctcacgttt tacggtaacg ttagtgagat catcagacat caatgtcaac
    10741 ttgcccggca cggcaatcac ccgctgccac tgacggcaat ccagggtatc gccctcttta
    10801 gtgacaatca ggctgcctat cgcttccgga ctgaccagcg cacgttgcgg ccctttggtc
    10861 tgccagtagc ccgccagcca gtccggcgcg ggggttttca cgacattgtt atagttttcg
    10921 acttcggcac agcccgccag caacatgagc gcgcttgcaa ttgcgagttt tttcatcatt
    10981 cgtcctgcat gagaagaaaa gaggattgtg gcattaaagc cctgatgtcg ccagcctttc
    11041 caggttcaga gagaaatttg atctaactat tatttttgtg aattattacc ccgaaaataa
    11101 atttgcatga tgtatgattc gcgattcttg tttcaaacct tctgagttca gaggctacat
    11161 tcatgtcatg gcagcaattc aaacacgcct ggttgattaa attctgggca cccatccctg
    11221 cggtcatcgc ggcgggtatt ctctccactt actattttgg cattactggc accttttggg
    11281 ctgtcacggg tgaatttacc cgttggggcg gtcagctcct gcagctgttt ggcgtccatg
    11341 ctgaagagtg gggttatttt aaaattatcc atctggaagg atcgccatta acccgcatcg
    11401 acgggatgat gatcctcggc atgtttggcg gctgctttgc cgcagcgctg tgggccaaca
    11461 atgtcaaact gcgcatgccg agcagccgta tccgcattat gcaggccatt attggcggca
    11521 ttatcgccgg ttttggcgcg cgtctggcaa tgggctgtaa cctggcggcg ttctttaccg
    11581 gcattcctca gttctcgctg catgcctggt tctttgccat cgccactgcc attggttcat
    11641 ggtttggcgc gcgctttacc ctgctgccca tcttccgtat tcccgtgaaa atgcagaaag
    11701 tttctgccgc ctcaccgctg acgcaaaaac cggatcaggc gcggcgtcgt tttcgtctcg
    11761 ggatgctggt ctttttcggc atgctgggct gggcgctgct cacagcgatg aaccaaccca
    11821 aactggggct ggcaatgctg tttggcgtcg gctttggttt actgattgaa cgtgcgcaaa
    11881 tctgctttac ttcagcgttc cgcgatatgt ggatcaccgg acgtacccat atggcgaaag
    11941 caatcattat tggtatggcg gtgagtgcca tcgggatctt cagttacgta cagttaggcg
    12001 ttgaacccaa aatcatgtgg gcgggaccaa acgcggtaat tggtggttta ctgtttggtt
    12061 ttggcatcgt gctggcaggc ggctgcgaaa ccggctggat gtaccgcgcg gtagaaggcc
    12121 aggtgcacta ctggtgggtc ggtctgggca acgtgatcgg ctcaacgatt ctggcgtatt
    12181 actgggatga tttcgctccg gcgctggcca ccgactggga caaaatcaac ctgctgaaaa
    12241 cctttggccc gatgggtggc ctgctggtga catatttgct gttgtttact gcgctgatgt
    12301 tgattatcgg ctgggaaaaa cgcttcttcc gccgtgcggc accgcagact gctaaggaga
    12361 tcgcatgaaa aatatcgttc ctgattaccg ccttgatatg gtgggtgaac cctgccctta
    12421 tccggcagtc gcaactcttg aggcgatgcc gcagttaaaa aaaggggaaa tcctggaagt
    12481 gatgagcgac tgtccacagt cgatcaataa tattccactg gatgcgcgta atcacggcta
    12541 tacggtgctg gatattcagc aagacgggcc gaccattcgt tatttaattc agaagtaatc
    12601 cctcattccc gctgtggccc acagcgggaa catcgttacc tttgtatcac atagatttta
    12661 cccgccccct ctcctattct tttcagtatg atctcaggag gtttctatgt gtggacgctt
    12721 tgaccaatcc caaacgcgtg tagattacct tgcgcttctc gcggaagata ttgaacgcga
    12781 tattccctat gatcccgaac ccattggcag atacaacgtc gcgccgggaa ccaaagtcct
    12841 tctgctcagt gaacgtgacg aacaccttca tctggatccg gttttctggg gatatgctcc
    12901 cggatggtgg gataaaccgc cactgattaa cgcccgcgta gaaactgccg caaccagtcg
    12961 catgtttaaa ccgctctggc aacatggtcg ggcaatctgt tttgccgatg gctggtttga
    13021 gtggaaaaaa gaaggcgaca aaaaacagcc ttattttatc tatcgcgctg atggacaacc
    13081 tgtttttatg gccgcgatag gtagcacacc gtttgagcgc ggtgacgaag ccgaaggatt
    13141 tttaattgtc actgcagcag cagaccaggg tctggtagat attcatgacc gccgcccgct
    13201 ggttctgtcg ccagaagctg cacaggaatg gatgcggcaa gagattggcg gtaaagaagc
    13261 atcagaaatt gctaccaatg gctgtgtgcc agctaaccag ttcacctggc acccggtatc
    13321 gcgcgcggta ggtaatgtca aaaaccaggg cgcggagtta attcaacctg tttgttgaca
    13381 tcctccacgc cctgaaggac gaggatccct gctacgttca ggctgtcgcc tgaatcattt
    13441 cggtgggttc ctgcttcaac gggcggcctg actgcaccat ctctccacag gcaagaacgg
    13501 cgtgccccgc agctaaaatg ttacgagcgc catttacatc ggcgttcgct gtatatccac
    13561 atacctggca tctgaattta ctttgtgaca ggcggttttc tttcgctgta tgaccacagc
    13621 acgcgcaacg ctggcttgtg tacgctggcg gaacagcaag cacctgaccg ccacgccaga
    13681 gctgcttgta ctcaagctgg cggcgcattt cataccagcc ctgatccagt atcgaacggt
    13741 ttaaacctga ttttgcccgg acattgcgcc ccggctggct gaccgtaccc gctgctgact
    13801 ttgacatgtt gctgaccttc aaatcctcaa tgactatcat tgcgtggttt ttgctgacgg
    13861 tcgttgtgac tttgtgaagg tagtccctgc ggatatttgc gatacaggaa tgcagtcgct
    13921 gtattttgcg tttctgcttc tgccagttgt tgctgaattt gaccttgcgg cttaactgtc
    13981 gctgaagtct cgccagcgtc ttctggtttt tctgaaaact gtttacaggc tcaaagactg
    14041 tgccatctga cagcgtggcg agtttagcca cgccagcatc cagcccgacc attgatgctg
    14101 aagggtgaac cggagttgat acttcacttt ctgtctgaat actgatgtac cacttaccgc
    14161 aggactggct gacagtgaca tttttcacaa cacccgtgac ctgacggctg ttccggtagc
    14221 gcatccagcc cagtttcggc agaaaaatac ggctgttttc ctgatcgagc ttaacgccct
    14281 gcgggtagcg gaatacatca ttctgtcccc gctttttgaa tcggggaaaa gcagcccgat
    14341 tctggaagaa gtttttgtaa gcccgctcaa ggtctttcag tgactgttgc aatggctgtg
    14401 agggagaatc tttaagccat tgcgtttcag tggcattttt ccactcaacc agccaggaag
    14461 ccattttccc gtaagggatg tatttattac cggcctcatg attctcattc tgacgtgcca
    14521 gagcacgatt gaaaacgaaa cgacatgc
//
LOCUS       DAPHDW010000112        14488 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.113, whole genome shotgun sequence.
ACCESSION   DAPHDW010000112 DAPHDW010000000
VERSION     DAPHDW010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.113"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(134..2401)
                     /gene="adiA"
                     /locus_tag="RH057_003342"
     CDS             complement(134..2401)
                     /gene="adiA"
                     /locus_tag="RH057_003342"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HDV3129665.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSTHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKRHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPMAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPGFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            complement(2600..3508)
                     /gene="melR"
                     /locus_tag="RH057_003343"
     CDS             complement(2600..3508)
                     /gene="melR"
                     /locus_tag="RH057_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HDV3129666.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSGWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            3791..5146
                     /gene="melA"
                     /locus_tag="RH057_003344"
     CDS             3791..5146
                     /gene="melA"
                     /locus_tag="RH057_003344"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HDV3129667.1"
                     /translation="MMTAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICEDMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPHIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKPGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPGWLH
                     R"
     gene            5249..6670
                     /gene="melB"
                     /locus_tag="RH057_003345"
     CDS             5249..6670
                     /gene="melB"
                     /locus_tag="RH057_003345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HDV3129668.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQPSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(6809..7438)
                     /locus_tag="RH057_003346"
     CDS             complement(6809..7438)
                     /locus_tag="RH057_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HDV3129669.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLMEVTPVIIV
                     VPLLLATAKRYPLTPLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVIFLARFHCRQLRRFGLITG"
     gene            complement(7561..9207)
                     /gene="fumB"
                     /locus_tag="RH057_003347"
     CDS             complement(7561..9207)
                     /gene="fumB"
                     /locus_tag="RH057_003347"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HDV3129670.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASVHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNPGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            complement(9285..10625)
                     /gene="dcuB"
                     /locus_tag="RH057_003348"
     CDS             complement(9285..10625)
                     /gene="dcuB"
                     /locus_tag="RH057_003348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HDV3129671.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            complement(11196..11915)
                     /gene="dcuR"
                     /locus_tag="RH057_003349"
     CDS             complement(11196..11915)
                     /gene="dcuR"
                     /locus_tag="RH057_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HDV3129672.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDTPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            complement(11912..13543)
                     /gene="dcuS"
                     /locus_tag="RH057_003350"
     CDS             complement(11912..13543)
                     /gene="dcuS"
                     /locus_tag="RH057_003350"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HDV3129673.1"
                     /translation="MRHSLPYRMLRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILKALNGKENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            13724..13954
                     /locus_tag="RH057_003351"
     CDS             13724..13954
                     /locus_tag="RH057_003351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4Fe-4S)-binding protein"
                     /protein_id="HDV3129674.1"
                     /translation="MDQALLDEGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKPWIMPDEVDVVTVVKVIDTCPSGALKYRHK"
     gene            13966..14238
                     /locus_tag="RH057_003352"
     CDS             13966..14238
                     /locus_tag="RH057_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3129675.1"
                     /translation="MEIREGHNKFYINDEQGKQIAEIVFVPTGENLAIIEHTDVDESL
                     KGQGIGKQLVAKVVEKMRREKRKIIPLCPFSKHEFDKTREYDDIRS"
BASE COUNT         3486 a         3473 c         3776 g         3753 t
ORIGIN      
        1 cggggtgggg ccgcgcaaaa agtatgacat ttttgtttgc agcaattttt atgaaaacgt
       61 tgccatactc aaaaggtcta aggccaggcg gcgtaatgtt atttaaacaa ttacgccttc
      121 agcggaatag tggttacgct ttcacgcaca taacgtggta aataccgtca ataatttcag
      181 tcccttcagt ttcgtgttca aatccaggga aatggtggtc ccaggattgc agcgagcgta
      241 aataacttac ttgcggactg tttttatcgc cgaagttttc accagacagc agcatcggga
      301 ttcctggcgg atacgggata actgagtttg ccgcgatgcg tcctggcaga ttttcaatgg
      361 ataccagttc gacattgttg tcgacaatcg cgttgtacgc ttcacgcggg gtgatttccg
      421 ccatcggcag gccggaatag gcttcgttca accgtgcgcc agggttgttt tctttcagcc
      481 aggcaaacat ggtgtcaccc agatcgtgaa tccccatgtt cgcgtaagtg tcaggatatt
      541 gttcaacaag ttccggcatc acctgcgcca gcggtgtgtt ggcgtcatag tggcgtttga
      601 aggagcaaag ggtgttaacc agagtccccc atttcccacg ggttacgccc atagagaaca
      661 ggaacataat ttggaagtca gtggtgcggg taggcacaat gccgtggcga ccaagccagg
      721 cagtgaccag cgccgccgga acaccggttt cttccagttc accatcttca cccattcccg
      781 gagcaaggat gctgacttta atcgggtcga gcatactcca gttatccgga atatctttga
      841 agccgtgcca gctttcgccc ggatgcatta cccagcagtc ctgaacggtg gtcagcagtt
      901 tggttggtgc gtcagcaaag tcataggttt tgccggtttg tgggtcggtg acgacttctt
      961 tgttccacgg tttgaagaac cagctaccgt cagcggtgaa ctctttatac agccgcgcca
     1021 tcgcctgacg aaaatcaacc gcttcgtcaa tcacttcttg tgtcagtgac aggccgctgt
     1081 tgccgtccat catcgacacc gccacgtcgt tggatgcgca gatggcatac agcggggagg
     1141 tggtggcatg catcatgtag gcctggttga agcgggagaa gttaatcgcc ccacgacctt
     1201 cacgtacatg aatataagaa gcctgtgaca gcgcattcag cagtttgtgg gtggagtggg
     1261 tggcgaaaac ggtaggaccg ttgtgatcgc caggttcgcc gcgcatggca tagtgatcgg
     1321 catagatcgg gttgaaacgt gcatagccgt accaggcttc gtcaaagtgc agacgatcgg
     1381 aggttttttc cagcagatcc tgcgcttctt tagcgttata acacacgccg tcataggtgc
     1441 agttggtcac cacgcagtaa gacggttttt gcccagcttt gtctttagtc agcgggcttt
     1501 cactgatttt cttctgcaag gtttcaggtt gcatttcctg cggatagatt ggcccgataa
     1561 tgccgtagcg gttgcggctt ggcaccatgt agactggttt cgcgcctgtc agcatcaagc
     1621 cttgttcgat ggatttatgg caattacggt caacgaccac gacatcgtta tcggtcatgc
     1681 aagcctgcat gatggtgcgg ttagagccgg aagtaccgac gactaccgac caggagcgat
     1741 cggcaccaaa tacgcgtgcg gcatattttt cgctttcgcc aaatgcgcca gtatggtcaa
     1801 gcaaagaacc gagggaagtt cgttcgatgc ccatatcggt gcggaacaga ttttctccat
     1861 agtagtcatg gtagaaacgt ccggcgggtg ttttggtaaa accaacgccg ccctggtggc
     1921 ctggcgctgc ccaggaatat tcatggatgt cgctatattt catcagcgcg ctgaacagtg
     1981 gcggcaacag ctgctggcgg tagcgggtca tcgcggcaac agcgcgtccg gcgataaagt
     2041 cggcggtatc ttccagaatc caggcgaatt catcgacaag ctccagcaga tcccggtcca
     2101 ttgcggcgag ggctttttcc cgatcgccca acaggaagac cggcacgttt tgttggcgct
     2161 catgaagctt accgatcaat tgtctgacgt tttgatgttc gtccggatgt tccatttgat
     2221 agctgaacat caggcagtca atggcttcgt ttgaagagag aatggcaaaa ccatcatcaa
     2281 aggaggtgga tttaatcacg gtaacatttt gctggcttaa agcatctgcc agacgctcaa
     2341 cggcgttacc gacccaggtg tcttgatgga gaaactcgct ttcaacaatt aatactttca
     2401 tcattgctta cccggttatg aaggaatctt cgttgcgggc aagtatcttc cggccttttc
     2461 gggctgtaaa gcgcgtgaaa aacaaacatt tttgaaatat ttagttttat aaacaatacg
     2521 ttatgtgatt attttaaatt attttcactg cttattatcg acggctaaac tatttttttg
     2581 gctgatactg atatcgtctt tagccgggaa acgtctggcg gcgctgttgg ctaagtttgc
     2641 ggtattgttg cggcgacatg ccgacatatt tgccgaacgt gctgtaaaaa cgactacttg
     2701 aacgaaagcc tgccgtcagg gcaatatcga gaatactttt atcggtatcg ctcagtaacg
     2761 cgcgaacgtg gttgatgcgc atcgcggtaa tgtactgttt catcgtcaat tgcatgaccc
     2821 gctggaatat ccccattgca tagttggcgt taagtttgac gtgctcagcc acatcgttga
     2881 tggtcagcgc ctgatcatag ttttcggcaa taaagcccag catctggcta acataaaatt
     2941 gcgcatggcg cgagacgctg tttttgtgtg tgcgcgaggt tttattgacc agaatcggtt
     3001 cccagccaga gaggctaaat cgcttgagca tcaggccaat ttcatcaatg gcgagctggc
     3061 gaatttgctc gttcggactg tttaattcct gctgccagcg gcgcacttca aacgggctaa
     3121 gttgctgtgt ggccagtgat ttgatcacca tgccgtgagt gacgtggtta atcaggtctt
     3181 tatccagcgg ccaggagaga aacagatgca tcggcagatt aaaaatcgcc atgctctgac
     3241 aggttccggt atctgttagt tggtgcggtg tacaggccca gaacagcgtg atatgaccct
     3301 gattgatatt cactttttca ttgttgatca ggtattccac atcgccatcg aaaggcacat
     3361 tcacttcgac ctgaccatgc cagtggctgg tgggcatgat atgcggtgcg cgaaactcaa
     3421 tctccatccg ctggtattcc gaatacagcg acagcgggct gcgggtctgt ttttcgtcgc
     3481 tgctgcacat aaacgtatct gtattcatgg atggctctct ttcctggaat atcagaatta
     3541 tggcaggagt gagggaggat gactgcgagt gggagcacgg ttttcaccct cttcccagag
     3601 aggcgagggg agcgtccgag catcatgagg ccgaaaactc tgcttttcag gtaacttatt
     3661 accataaact cagatttact gctgcttcac gcaggatctg agtttatggg aatgctcaac
     3721 ctggaagccg gaggttttct gcagattcgc ctgccatgat gaagttattc aagcaagcca
     3781 ggagatctgc atgatgactg cacccaaaat tacatttatc ggcgctggtt cgacgatttt
     3841 cgttaaaaat attcttggtg atgtgttcca tcgcgaggcg ctgaaaacgg cgcatattgc
     3901 cctgatggac attgatccca cccgcctgga agagtcgcat attgtggtgc gtaagctgat
     3961 ggattcagca ggggccagcg gcaaaatcac ctgccacacc caacagaaag aagccttaga
     4021 ggatgccgat tttgtcgtgg tggcatttca gattggcggt tatgaacctt gcacggtgac
     4081 tgatttcgag gtctgtaagc ggcatggtct ggaacaaacc attgccgata cgttggggcc
     4141 gggcggtatt atgcgcgcgc tacgtaccat tccgcatctg tggcaaattt gcgaggacat
     4201 gacggaagtc tgccccgatg ccaccatgct caactatgtt aacccaatgg cgatgaatac
     4261 ctgggcgatg tatgcccgct atccgcatat caaacaggtc gggctgtgcc attcggtgca
     4321 gggaacggcg gaagagctgg cgcgtgacct caatatcgac ccagctacgc tgcgttaccg
     4381 ttgcgcaggt atcaaccata tggcgtttta cctggagctg gagcgcaaaa ccgccgacgg
     4441 cagttatgtg aatctctacc cggaactgct ggcggcttat gaagcagggc aggcaccgaa
     4501 gccgaatatt catggtaata ctcgctgcca gaatattgtg cgctacgaaa tgttcaaaaa
     4561 gctgggctac ttcgtcacgg aatcgtcaga acattttgcc gaatatacgc cgtggtttat
     4621 taagccaggt cgtgaggatt tgattgagcg ttataaagta ccgctggatg agtacccgaa
     4681 acgctgcgtc gagcagctgg cgaactggca taaagagctg gaggagtata aaaacgcctc
     4741 ccggattgat attaaaccgt cacgggaata tgccagcaca atcatgaacg ctatctggac
     4801 tggcgagccg agtgtgattt acggcaacgt ccgtaacgat ggtttgattg ataacctgcc
     4861 acaaggatgt tgcgtggaag tagcctgtct ggttgatgct aatggcattc agccaaccaa
     4921 agtcggtacg ctaccttcgc atctggccgc cctgatgcaa accaacatca acgtacagac
     4981 gctgctgacc gaagctattc ttacggaaaa tcgcgaccgt gtttaccacg ccgcgatgat
     5041 ggacccgcat actgccgccg tgctgggcat tgacgaaata tatgctcttg ttgacgacct
     5101 gattgccgct cacggcgact ggctgccagg ctggttgcac cgttaaaacg cgactaaacg
     5161 ctactgcgcc gggggattta ttccggcgca cacctctgac gataccaata acagaaggcg
     5221 ggcgttggta acagcgaccc gataccctat gagcatttca atgactacaa aactcagtta
     5281 tggatttgga gcgttcggga aggattttgc gatcggcatt gtgtatatgt acctcatgta
     5341 ttactacacc gatgtcgttg ggctgtctgt gggtttggtc ggtactttgt ttctggtggc
     5401 gaggatctgg gatgctatta acgatccgat tatgggatgg attgtaaatg ctacgcgatc
     5461 gcgatggggt aagttcaaac cctggatcct gatcggtacg ttggcaaact ctgtaatctt
     5521 atttctcctc tttagtgcgc atctgtttga aggtactact cagattgtct ttgtttgcgt
     5581 gacctacatc ctctggggca tgacttacac cattatggat attcccttct ggtcgctggt
     5641 tccaaccatc acgctcgata aacgtgagcg cgaacaactg gttccttatc cgcgtttttt
     5701 tgccagtctg gcaggctttg ttacggcagg tgtgacgcta ccatttgtta attatgtcgg
     5761 cggtggcgat cggggatttg gctttcagat gttcactctg gtactaatcg ccttttttat
     5821 tgtttcaacc atcatcactc tgcgcaatgt gcatgaagtc ttttcgtcag acaatcaacc
     5881 gtctgctgaa ggaagccatc tgacacttaa agccatcgtt gcgctaattt ataaaaacga
     5941 tcagctttca tgcctcttgg gtatggctct tgcttataat gtagccagca acattattac
     6001 cggatttgct atctattatt tctcatatgt tatcggtgat gcggatttgt tcccctatta
     6061 tctgtcgtat gcgggagctg ctaacctggt gacgttagta ttcttcccac gcttagttaa
     6121 atcattatcc cgacgcattt tatgggccgg agcatctatt cttccggtgt taagctgtgg
     6181 tgttctcctg ttaatggcat taatgagcta tcacaacgtc gtcctcattg tgattgcggg
     6241 tattttgctg aatgtgggaa cggcgctttt ctgggtatta caggtcatca tggtggcaga
     6301 taccgttgat tacggtgaat ataaactgca cgtacgctgt gaaagtatcg cttactccgt
     6361 gcagactatg gtggtgaagg gcggttcagc ctttgcggct tttttcattg cggttgtgtt
     6421 agggatgatt ggctatgtac cgaatgttga acagtctacg caagccctat taggtatgca
     6481 gtttattatg attgctctac caactctgtt tttcatggta acgctgattc tctacttccg
     6541 tttctatcgc ctcaatggcg acacgctgcg caggatccag attcatctgc tggataaata
     6601 tcgcaaagta ccgcccgagc ctgttcatgc tgatattccg gtcggtgcag tgagtgatgt
     6661 gaaagcctga cgtggacaat agctaacgaa aatggcctga tgcgatatgt ttatcaggcc
     6721 acgtcttaat gtaatgcttt ggatttgcaa attttgtaga ccggataagg aattcacgcc
     6781 gcatccggca tcaacaaagc gcacgttgtt atccggttat caagccaaag cgccgtagct
     6841 ggcggcaatg aaagcgagcg aggaatatca ccgtcgttaa tgcgccaagc agcgcgcaga
     6901 acatatcgga ttgcgtgtcc cactggtcgc cctgggtgcc gagaaaatca tccgctccct
     6961 ggcccattgc cagcgccgcc caccactcga ttaattcata catcgcgctt atcgccagcg
     7021 ccacgcagca gaccaggaac gccaccattt tacgtccgcg cacgtacatc ccgcgcacga
     7081 gaatttctcg tgccaccagt gcaggcacca gcccctggaa aaaatgcccc agcttgtcat
     7141 acggattacg gctcaacccc agccattcct gcacctcaaa accaacgggg actttcgcgt
     7201 aggtgtattg cccgccgacc atcaggatga tggcgtgaaa gaaaatgagc gtatagagca
     7261 gcggcgttaa cggataacgt ttagcggtgg caagcagtag cggcacgaca ataatcaccg
     7321 gtgtcacttc catcagccag gtgagtttgt catgggccga aatgccggta taaatcagga
     7381 ttaacgttag cgccagtgcg ccggtgttaa gaattaacgg cttgagtgtg cgggtcatgg
     7441 tgagttcgca aatcagggaa aatactgact attcaccagc aagcgtgaaa ttacaatcgc
     7501 aaaagaggtg gcccaggggg atcacctggc agcatgctgc caggcgctgg gccgaagagg
     7561 ttacttagtg cagttcgcgc actgtttgtt gacgatttgc tggaagaagt cgttaccttt
     7621 gtcatcgacc aggataaacg ccgggaaatc ttctacttcg attttccaga tagcttccat
     7681 acccagctcc ggataagcga cgcattccag atgcttgatg ctctgctgcg ccagtaccgc
     7741 cgccggaccg ccgatgctac cgaggtagaa gccgccgtgt ttatgacacg cgtcggtaac
     7801 ctgctgactg cggttacctt tcgccagcat gatcatgctg ccgccgtggg attgcagcag
     7861 atccacgtag gagtccatac ggcctgcggt ggttgggcca agtgaacctg atggataacc
     7921 ggcaggggtt ttcgccggac ccgcgtagta gatcgggtga tctttgatgt actgcggaag
     7981 ttctttaccg gcgtcaatca gctctttcag cttggcgtgt gcaatatctc ggcccacgat
     8041 aatggtgccg gtgagcgaca aacgagtgga taccgggtat tgcgaaagct gggcgaggat
     8101 ctctttcatc gggcggttaa ggtcaacttt caccgcttcg ccttcaccgg cctggcgcag
     8161 ttcttgtgga atgtactggc ctgggttgtg ttccagtttt tcgatccaga taccttcgcg
     8221 gttgattttc gctttaatgt tacggtcagc ggagcaggag acgcccatgc cgaccgggca
     8281 ggatgcgccg tgacgtggca gacggataac gcgaatgtcg tgcgcgaagt atttaccgcc
     8341 aaactgcgcg ccaagaccga gtttctgggc ctcttccagc agttcctgtt ccagctggac
     8401 atcgcggaac gcctgaccat gttcgttccc ttccgtcggc agttcatcgt aatagtgaac
     8461 gcttgctaac ttgacggttt tcaggttggt ttccgcagac gtaccgccaa tcacaaacgc
     8521 gatatggtac ggcgggcagg ctgcagtacc gagggtacgc attttctcga cgaggaagtt
     8581 tttcagtttg ccgggagtca gcagggcttt ggtttcctgg tagagatacg ttttgttggc
     8641 agagccgccg cctttcgcaa cgcaaaggaa tttgtactca tcgccatcta ccgcgtacag
     8701 gtcgatttgc gcaggcaggt tagtgccggt gttgacctct ttgtacatgt ccagcgccgc
     8761 attctgtgaa tagcgcaggt tatcttcgat ataggtgtta tagacgcctt tcgacagcgc
     8821 ttcttcatca ccgccgccgg tccacacgcg ctggcctttt ttaccgacga tgatcgcggt
     8881 gccggtatcc tggcaggtcg gcagcacgcc tttggcggcg atttcggagt ttcttaagaa
     8941 ttgcagcgcc acgtacttgt cgttttcgct ggcttctgga tcgtgaagaa tagccgcaac
     9001 ctgtttctgg tgtgccgggc ggagcataaa agaagcgtcg tgaaaggctt gctgcgccag
     9061 cagggtcagg gcttctggtt ccactttcag gatggtttcg ccgtcgaagt cggcaacgct
     9121 aacgtaatcg gaagtgagta gatagtattc ggtattgtct ttccccatcg ggaaaggtgc
     9181 ctggtagata aagggtttgt ttgacatagc ttccagcctg taactctgta tttgttattc
     9241 gaaaatggcg tgccgcgtag tcacgggcgg cacgcaaagt gcatttataa gaacccgtac
     9301 atcgcggcga agatccagcc gaagacgcac gatacgctca caccaatcaa ccccggcaga
     9361 ataaagctgt ggttgatgac gaagcgaccg atgtgggtgg tgccggaacg gtcaaactga
     9421 atcgctgcca gatcgctcgg ataagtcggc aggatgtaat aaccgtagca agccggtgct
     9481 gaagccacga tgtatgccgg atcaacgccg atcgccagcg cgaccggaac aatcgccgcc
     9541 agcgccgcag cctgagagtt tacaaacttg gaaaccagca gcagaacaat ggcataggcc
     9601 cacggatact ctttcaccat ttcacccagt acgccctgaa tttcagacat atgcgcaccg
     9661 aacatggttt ctgccatcca tgcgataccg tacaccgcca cgatggcgat cataccggaa
     9721 cggaagactt cgttttttga gatagacgcg ggattggttt tggtcaggat aataatcagc
     9781 gccccggtca gcagcataaa catctgaata accagtacca tcgacagcgg tttgccgccg
     9841 aaggatggac gcaggtccga atcagcacca agaagggcga ctacagcgat tgcaccgagg
     9901 aaaatccaca ttgccagcca gttgcttttc ggcagttttt tatcgagcag cgtcgcggta
     9961 tcaccgtaaa catactcacg gttttccggt acggagatga atttctggaa ctcttcgtct
    10021 ttatccagat ctttaccgcg gaaccagctg aagataccga tcgccaggat accgattaac
    10081 gtcgatggaa tggtgattgc cagcaggtcg aggaactcaa gatggcgacc atcaaaggtg
    10141 acattaccca gcattgcaac cagagacacg accgcaaccg acaccggact ggcgataatc
    10201 cccatctgtg caccgataga acttgccgcc atcggacgtt ccggacggat gttgttctta
    10261 atggcgacgt cgtagatgat cggcagaatg gtgtaaacca catgacccgt accgcaaaga
    10321 atggtcagtg tacaggtcac aaacggcgcg acaattgaga catatttcgg gttgcggcgc
    10381 agcagcttct cggcaatttg cagcatgaca tcaagaccgc ccgaagcttg caaggtcgcc
    10441 gatgccgcca ccaccgcaat gataaccagc atgacatcaa ccggtggttt acctggctga
    10501 aggtggaaga cgaagaccag aatgaccaga ccgataccgc ctaataaacc cagcgcgata
    10561 ccaccctttc tggcaccata aaacagacat atcagtatta tgataagttg gatagtaaat
    10621 aacatgtgtg aaccctcgcg ataatcctat ttaaattttt gctgaataga tcacagtcac
    10681 gttctgtttt gtatgaactg ttttcagagc ctgactaaat atccgtttgg tcgttaccgg
    10741 ctttagcaaa tacctcacag taaatattgg ctgattaaag cagcggcaaa tttatgcact
    10801 gtgataagcg gctttttcag agagaaaaac tctctggcga tgggtttata gtttgtactg
    10861 cgcagatgtt aatgttttgt ttgctgttat cttattgata tttatcggtc tattttttgt
    10921 tctcagggca gatatcatta ggtttaaaga ttaatctgat ctacccattt gtgggtaaaa
    10981 atacacataa tgcgggtgac ataatagtta attaactttt gttatcgttt tgaaattaaa
    11041 aacaccgttc acctgaagag atattaattt ttagcgatga tggagggata attatatttg
    11101 atctggcaca agttttactg atgaaggatg taacttgtgc caggggtatt ttgcattacg
    11161 gtaataatta ttacttacag ataaccagcg atccgttatt ggcaatattg tttcagtagt
    11221 gagtagtgtt ctgcctgaat acggtaacgg taaaccggac gccccgtgac gccataatgg
    11281 atactggtga acaagatgtg gcagttgacc agccagatga ggtatttacg gcaggaaaca
    11341 cgcgagatgt taacctcgtt agccagctcg tcggttgaaa actcatagtc ctgatgcgcg
    11401 tcaatccact ggcacagcgt gcgtaacgtc tgcggcgtta agccttttgg caagcgacga
    11461 ggatcctgtt cgttggagct gctgccgtgg attagctgat caagctcggc ctggtcataa
    11521 tactgatgtt tttccagcgc cattttcttt tgccgccagc cggtgagcgc ctcttcaaag
    11581 cgggaagcct ggaagggttt gatcaggtaa tccacgacac cgtaatgcag cgaatcttta
    11641 atggttgccg catcggctgc ggaggagatg acaatcacat cacttttgca acgcgcgtta
    11701 tgcaggacag gcagtaaatc gagcccgttc tctttttgca tatagatatc gagcaatatc
    11761 aggtcgatag gcgtatcgct attgaagata atctctttgg ctttctccag cgtcgaggct
    11821 gttccacagc attgaaagcc tgggatttgt gctacgtatc ggcgattcag ctccgcgacc
    11881 attgcgtcgt catcgataat taatacattg atcatctgtt cgacctctcc ccgtcccagg
    11941 gtatctggac aaaaaattgt gtgaaaatcc cgggttccga ttccacggcg atgctgccgc
    12001 cgagattttc tacctgttgt ttgacaagtg ctaaaccgac gcctcgctcg cttccttttg
    12061 tcgagacacc tttgtcaaaa atgtgatcga ttttgtcggg tgcgatcccc ggtccatcat
    12121 cgttaacttc gcagtgcagc cagccgtgac ggtagtgcaa tgttacgcta atttcgccac
    12181 cgggttccgg ccctaatgct tccagcgcgt tttctatcag atttcccaat gtggtaatca
    12241 gcgtcgcgac ctggtcctca ctgccgctgt ctggcagctg gctttcactg tttaaaatca
    12301 gcgtatggcc taaatcggtc gcgcgattaa tcttgctgat taaaaaacca gcgataaccg
    12361 gagatttgat cttacccagc agagagccaa tctcttcctg atagttattg gctgttttga
    12421 gaatgtaatc ttccaactgc ttataactct tcagatgcaa taatccgaga atcacatgca
    12481 atttattcat aaattcgtgg gatcgttcac gaagtgcatc agcatagttg accagaccgt
    12541 cgagtcgctg catcagttta cgaacttcag ttttgtccct gaaggttgaa atggcaccga
    12601 tgataacgcc attactgcgc accggaacgg tgttgatcag taatagccga tctttaatcg
    12661 taatctcttc gtcgcggcgc ggcgtaccgt cgcgtaacac ttccgagaca tctaccacct
    12721 gtgaccatga gtggctgagc gtcgacagtt tctcatcgtc ctgcgactta cggtaattca
    12781 gcaattcttg tgcggcatcg ttgatcagcg tgacctcgcc gcgatcgtcc acggcaacga
    12841 cgccttcttt gatagactgc aacatggcct ggcgctgctc aaacagcgtg gagatttcgt
    12901 agggttccag gccgaaaagg atttttttca gtaccttaac cagaatgcag gtgccaatca
    12961 gtccgaccag catgccaaat aataccgacc agataatgct ccagcgactg tcattgattt
    13021 gttgggtcac gcggcttaac tcaaggccga tcgccaccac gccaatttgt ttatgatttt
    13081 catcgtagat gggggtaaat acgcgtaaag cctgcgccag aaaaccgcga ttgatagcga
    13141 cattttcttt gccattcagc gccttaagga tgtcatcacc tttaaatggc tgaccaatac
    13201 gctgggcttc aggatgcgag tagcgaagac tttgcatatc ggtaacgaca ataaacagca
    13261 gatcgttgcg tttgcgcacg gcttccgcga tggcctggat tccgctctcc tgcggttttt
    13321 tctgcaagcc ctgacggatt tccggcgagt cggcgagggt acgcgccact gccagtgcct
    13381 tgttggctag cccgtctcgc gtcatatcac tgatttgcga gaagtaaatc agatgcacca
    13441 ccaatagcac cgagaacagt accgcactga ccattaagat cactgtggta ctcaatttca
    13501 tcggacgttt gcgtaacatg cggtagggca atgaatgtct catcagcttc cttgtgtgac
    13561 aaatttctta agcattatct ctgatgaggc gggtaattca aagggagtaa gaatgattgg
    13621 ctatataggg gaagagactc tggcaacgga aactgccagt gctgtatgaa gattccaggg
    13681 ctatgcttat agcgataatc atactgatga gagagggaaa gtcatggatc aggcgctact
    13741 ggacgagggt tatcgctgtt ataccggcga aaagatcgat gtctatttca acactgcgat
    13801 atgtcagcat tctggcaatt gcgtacgtgg caacggcaag ttatttaatc tcaaacgaaa
    13861 gccgtggatc atgccggatg aagtcgacgt cgtcactgtg gttaaagtga ttgatacgtg
    13921 cccgagcggc gcgctgaaat accgtcataa ataagcgagg gtaaaatgga aatacgcgaa
    13981 ggccacaata aattttacat taatgacgaa cagggcaagc aaatcgctga aattgtcttt
    14041 gtgccgaccg gagagaattt agcgattatc gaacataccg atgtcgatga aagcctgaaa
    14101 gggcagggga ttggtaaaca gctggttgcg aaagtcgtgg aaaaaatgcg tcgggaaaaa
    14161 cgaaaaatta tcccactatg cccattttcg aaacacgaat ttgataaaac gcgggagtat
    14221 gatgatattc gcagttgatg ggagagtaca gagtcacgat atttttcatg ctctccgcgg
    14281 agtgatgcag gagagcatct gaagggtagg gggatgcaca aagaatgggc agagagagcg
    14341 tttttttgtc ccaagtcatc ccctttactg agcaaaaaaa agaatatctc ctatatgaga
    14401 atcatcaatc ggggttaata agttttgcgt ccccagagcg tttaatattg ataggagtca
    14461 tattatggaa ggtaaaaaca agttcaat
//
LOCUS       DAPHDW010000113        14361 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.114, whole genome shotgun sequence.
ACCESSION   DAPHDW010000113 DAPHDW010000000
VERSION     DAPHDW010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.114"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            21..587
                     /locus_tag="RH057_003353"
     CDS             21..587
                     /locus_tag="RH057_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3129676.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLRLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            638..3097
                     /locus_tag="RH057_003354"
     CDS             638..3097
                     /locus_tag="RH057_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3129677.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNGQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNITIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNQTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSSNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYGGLLLSGDD
                     YHSAAIGIGQNMLWHGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            3112..3843
                     /locus_tag="RH057_003355"
     CDS             3112..3843
                     /locus_tag="RH057_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HDV3129678.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            3840..4901
                     /locus_tag="RH057_003356"
     CDS             3840..4901
                     /locus_tag="RH057_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3129679.1"
                     /translation="MSAGKGVLLVICLLFLPLKSALALNCYFGSSGGSVEKSEAIQPF
                     AVPGNAKLGDKIWESDDIKIPVYCDNNTNGNFESEHVYAWVNPYPGVQDRYYQLGVTY
                     NGVDYDANQGKSRIDTNQCIDSKNIDIYTPEQIIAMGWQNKICSGDPANIHMSRTFLA
                     RMRLYVKIREMPPHDYQSTLSDYIVVQFDGAGSVNEDPTAQNLKYHITGLENIRVLDC
                     SVNFSISPETQVIDFGKFNLLDIRRHTMSKTFSIKTTKSQNDQCTDGFKVSSSFYTEE
                     TLVEEDKALLIGNGLKLRLLDENASPYTFNKYAEYADFTSDMLIYEKTYTAELSSIAG
                     PPIEAGPFDTVVLFKINYN"
     gene            5053..6099
                     /locus_tag="RH057_003357"
     CDS             5053..6099
                     /locus_tag="RH057_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HDV3129680.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(6096..6887)
                     /gene="nei"
                     /locus_tag="RH057_003358"
     CDS             complement(6096..6887)
                     /gene="nei"
                     /locus_tag="RH057_003358"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HDV3129681.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFSNDLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQAADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(6923..7657)
                     /gene="pxpA"
                     /locus_tag="RH057_003359"
     CDS             complement(6923..7657)
                     /gene="pxpA"
                     /locus_tag="RH057_003359"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HDV3129682.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALILVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVTAQTVCLH
                     GDGEHALAFARRLRSTFAEKEIVVAA"
     gene            complement(7647..8579)
                     /gene="pxpC"
                     /locus_tag="RH057_003360"
     CDS             complement(7647..8579)
                     /gene="pxpC"
                     /locus_tag="RH057_003360"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
                     /protein_id="HDV3129683.1"
                     /translation="MLKIIRAGMYTTVQDGGRHGFRQSGISHCGALDMPALRIANLLV
                     GNDANAPALEITLGQLTVEFETDGWFALTGAGCEARLDDNAVWTGWRLPMKAGQRLTL
                     KRPQHGMRSYLAVAGGIDVPPVMGSCSTDLKVGIGGLEGRLLKDGDRLPIGKAKRDFM
                     EAQGVKQLLWGNRIRALPGPEYHEFDRASQDAFWRSPWQLSSQSNRMGYRLQGQILKR
                     TTDRELLSHGLLPGVVQVPHNGQPIVLMNDAQTTGGYPRIACIIEADMYHLAQIPLGQ
                     PIHFVQCSLEEALKARQDQQRYFEQLAWRLHNEN"
     gene            complement(8573..9229)
                     /gene="pxpB"
                     /locus_tag="RH057_003361"
     CDS             complement(8573..9229)
                     /gene="pxpB"
                     /locus_tag="RH057_003361"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HDV3129684.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAHCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGVC
                     "
     gene            complement(9252..9995)
                     /gene="ybgI"
                     /locus_tag="RH057_003362"
     CDS             complement(9252..9995)
                     /gene="ybgI"
                     /locus_tag="RH057_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HDV3129685.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLVADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGLELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            10266..11747
                     /gene="dtpD"
                     /locus_tag="RH057_003363"
     CDS             10266..11747
                     /gene="dtpD"
                     /locus_tag="RH057_003363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HDV3129686.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(11789..13207)
                     /gene="phrB"
                     /locus_tag="RH057_003364"
     CDS             complement(11789..13207)
                     /gene="phrB"
                     /locus_tag="RH057_003364"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HDV3129687.1"
                     /translation="MITHLVWFRQDLRLHDNLALAAACRNSSARVLALYIATPRQWAT
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVEVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIEPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCQN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIVDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVDHKEARLRTLAAYEEARKGA"
     gene            complement(13204..13713)
                     /locus_tag="RH057_003365"
     CDS             complement(13204..13713)
                     /locus_tag="RH057_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HDV3129688.1"
                     /translation="MNQQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYYQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            complement(13864..14361)
                     /locus_tag="RH057_003366"
                     /pseudo
     CDS             complement(13864..14361)
                     /locus_tag="RH057_003366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         3462 a         3848 c         3487 g         3564 t
ORIGIN      
        1 ttctgttaaa ggactccgtt atgtttaaag gacaaaaaac attggccgca ctggccgtat
       61 ctctgctgtt cacagcacct gtttatgcgg ctgatgaagg atccggtgaa attcacttta
      121 aaggtgaagt tattgaagca ccgtgtgaaa tacatcagga tgatattgat aaagaggttg
      181 aactcggtca ggtgaccacc agccacatta atcagtcaca tcacagcgat gccgttgctg
      241 tcgacctgcg cttagtcaac tgtgatctgg aaaactccag caacggttcc ggtggcaaga
      301 tttctaaagt tgcagtcacc tttgatagct cagcgaaaac caccggcgca gatccgattc
      361 tcaacaacac cagcacgggt gaagccaccg gcgtcggcgt acgtttaatg aataaagatc
      421 aaagcaatat cgttttaggc accgctactc cagatatcga cctggctccg acctccagcg
      481 aacaaacgct gaatttcttt gcctggatgg aacaaattga tcaggctaca cctgtaacgc
      541 caggcgcagt tacggcgaat gcaacctacg tgctggatta taaataagta ttactttggc
      601 ggcgctatcg ccgcctttca taaaatattt atcaggtatg gacaccgtga atatttatcg
      661 actctctttt gtatcctgcc tggtcgtggc gatgccttgc gcattggcgg tcgaattcaa
      721 ccttaatgtt ctcgataaat caatgcgcga ccgcattgat atttcattat taaaggaaaa
      781 aggagtcatt gctcccggtg agtattttgt tagcgttgcg gtaaataata accaaatcag
      841 taacgggcaa aagattaact ggcacaaaaa tgacgataaa accattccgt gcatcaatga
      901 tttactggtc gataaatttg gcttaaaacc tgaagtccgt cagtcgttac cattgataaa
      961 tcagtgcgtc gattttagct cccgacctga aatgctcttc aatttcgatc aagccaatca
     1021 gcaactaaat atcaccattc cgcaagcctg gctggcgtgg cactcagaaa actggacccc
     1081 accctccaca tggaaagaag gtgtcgccgg tgtcctgatg gattacaact tgtttgccag
     1141 cagctaccgc ccacaggatg gcagcagcag cactaatctt aacgcctatg gaacagccgg
     1201 aatcaacacg ggggcatggc gcttacgcag tgattatcag ttgaatcaga ctgatagcga
     1261 tgataaccat gaacagtcag gcgaaatatc acgtacctat ctttttcgcc cgttaccgca
     1321 attaggttca aaattaaccc tcggcgaaac ggattttagt tccaatattt tcgacggttt
     1381 ttcttatacc ggcgcggcac tggcaagtga cgatcgaatg ttgccatggg aactacgcgg
     1441 ctacgcccca caaattagcg gtattgcaca gaccaatgcc acggtgacga tcagtcaatc
     1501 aggccgcgtc atttaccaga aaaaagtccc accaggccca tttatcattg acgaccttaa
     1561 tcagtctgtt cagggcacac tggatgtcaa agtgacggaa gaagatggtc gggtgaacaa
     1621 tttccaggtt tcggcagcat cgacgccctt cctgactcgt cagggacagg ttcgctataa
     1681 actggccgcg ggtcagccac ggccctccat gtcacatcaa actgaaaatg aaaccttttt
     1741 tagcaatgaa gtttcctggg ggatgctgtc aaacacctcg ctgtacggcg gcctgctgct
     1801 ttctggtgat gactaccatt ctgccgcaat aggtatcggg caaaatatgc tgtggcatgg
     1861 cgcgctgtcg tttgatgtca cgtgggccag tagtcacttt gatactcagc aggacgagcg
     1921 gggcttaagc taccgtttta attacagcaa acaagtggat gccaccaaca gcacgatttc
     1981 cctcgccgct tatcgtttct ccgatcgtca ttttcacagc tacgccaact atctggatca
     2041 caaatacaac gacagcgatg cgcaggacga aaaacagacg atcagcttat ctgtgggcca
     2101 accgattacc ccactaaacc tcaatcttta cgccaacctg ctacatcaaa cctggtggaa
     2161 tgcagacgcc tccacgaccg ccaacatcac ggcaggtttt aatgttgata ttggtgactg
     2221 gagagatatc tctatttcga cgtcattcaa tacaacccat tacgaagata aagatcgcga
     2281 caaccagatt tacctgtcga tttcgctccc cttcggtaac ggtggtcggg ttggctatga
     2341 catgcaaaac agtagccaca gcaccacaca ccgcatgtcg tggaacgata cgctggatga
     2401 acgtaatagc tggggcatgt ctgccggact gcaatccgac cgtccggaca atggagccca
     2461 ggtgagcggt aactatcagc acctgagttc agcgggtgag tgggatattt ctggtaccta
     2521 tgccgccaat gattacagtt ccgtcagcag cagctggagc ggttctttca ccgcaaccca
     2581 atatggtgca gcgtttcatc gccgcagctc caccaatgaa ccacgcctga tggtcagcac
     2641 cgatggcgtg gcagatattc cggttcaggg caatctcgac tacaccaacc attttggcat
     2701 tgcggtggtg ccgttgattt ccagttacca gccttccacc gtggcggtga acatgaatga
     2761 cttacccgac ggcgtaacag ttgcagaaaa cgttatcaaa gaaacgtgga ttgaaggcgc
     2821 gataggttac aaatcactgg cttcccgttc cggtaaagac gttaacgtca tcattcgcaa
     2881 cgccagcggt cagtttcctc ccctcggagc ggatatccgc caggatgaca gcggcattag
     2941 cgtggggatg gttggcgagg aaggacatgc ctggttaagc ggagtcgctg aaaatcaaaa
     3001 gtttaccgtg gtctggggtg atagccagca ttgctcgctc catcttcctg aacatatgga
     3061 agacaccgca aatcgcctga ttttaccttg tcattaatta aggaaatgaa aatgacattt
     3121 atgaaaggac tacccctttt gttgttggtt gccagtctgt gcagccacgc tgcactacaa
     3181 cccgatcgca ctcgtattgt gtttaacgct aatgataaag ctaccagtct gcgggtggat
     3241 aatcgtagcg ataaactccc ctacctcgct tattcctggc tcgaaaatga aaaaggggag
     3301 aaaagcgatg atcttctggt cgccctgccg ccgatacaac gtctggaacc gaaagcaaca
     3361 acgcaggtac ggatagtaaa gcaagcctcg accacaaaat taccgggcga tcgcgaaacg
     3421 ctatttttct acaatatgcg tgaaattcct cccgccccgg aaaaaaacag cgaccatgcc
     3481 gttctgcaag tcgccattca gagtcgtatc aaggtgttct ggcgaccggc tgcattacgc
     3541 aagaaagcag gagaaaaggt cgaactgcag ttacaggtca gccagcaggg caaccaactg
     3601 acactgaaaa atcctaccgc gtattatctg accattgcct acctgggacg aaatgaaaaa
     3661 ggcgttctcc ccggcttcaa aaccgtgatg gttgcgcctt ttagtacggt caacacaaac
     3721 accggaaatt atagcggcag ccagttttat ctcggttata tggatgatta cggcgcgtta
     3781 cgcatgacga cgctcaactg cagcggacaa tgccatttac aagccgtgga ggcgaagaaa
     3841 tgagtgctgg caagggagtg ttgctcgtca tctgtctgtt atttctgccg ttgaagtctg
     3901 cgcttgccct gaactgctac tttggttcat ccggtggctc ggtagaaaaa tcagaagcga
     3961 ttcaaccttt tgctgtacca ggcaatgcca aactcggcga taagatttgg gaatctgacg
     4021 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caattttgaa agtgagcatg
     4081 tttatgcctg ggtaaacccc tacccggggg tgcaggatcg ctattatcaa ctcggcgtaa
     4141 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
     4201 gtattgacag taagaacatt gacatttata cccctgagca gattatcgcg atgggatggc
     4261 agaacaaaat ttgctcgggt gatcccgcta atattcacat gtcgcgtacc tttctcgctc
     4321 gcatgcggtt atacgtcaaa atacgagaaa tgccgccgca tgattatcaa agtacgctta
     4381 gcgactatat cgtcgtgcaa tttgacggtg ccggtagcgt taacgaagac cctactgccc
     4441 aaaacctgaa atatcatatt actggtctgg aaaacattcg cgtgctggat tgcagcgtca
     4501 atttttccat ttcaccggaa acacaggtga ttgattttgg taaatttaat ttgctggata
     4561 ttcgtcgcca cacaatgtcg aaaacattca gtattaagac gacaaaatca caaaatgacc
     4621 aatgcaccga cggatttaaa gtcagttcct ccttttatac cgaagaaacg ttggttgaag
     4681 aagataaagc attgttgatt ggtaatggtc tgaagctacg tttgctggat gaaaacgcct
     4741 cgccttatac cttcaataaa tatgctgaat acgccgattt caccagcgat atgctgatct
     4801 acgaaaaaac ctatacggct gaactttcgt ccattgcagg cccccccatc gaggctggcc
     4861 ccttcgatac agtggtgctt tttaagatta actataactg atttgccatg atgccctttg
     4921 cagccggaaa gggcatctct ttcagaaaat tgctgatttt ccccttttcc ttgattgctg
     4981 ttattttttc ctggcccggc tttctttatg ctgtatcgat acaccaaaga gaaaataatg
     5041 agggagcgaa ggatgccagt tttgcagtgg ggaatgttat gtgtgctatc acttctcctt
     5101 tctattggtt tcctcgcggt acatctcccg gcggcgctat tacttgggcc gatgatcgcc
     5161 gggatcatct tcagtatgcg cggaattact ctgcaacttc cccgctccgc ttttctcgcc
     5221 gcgcaggcca ttcttggctg catgattgcg caaaacctca ctggttcgat tctcaccacg
     5281 ctggccgcca actggccgat cgtgcttgct attttactgg tgacgctgct ttccagcgcc
     5341 atcgtgggtt ggttattggt gcgctatagc tcactgcctg gaaataccgg tgcctggggt
     5401 tcttctccag gcggcgcagc ggcaatggtc gctatggcgc aagattacgg cgcagatatt
     5461 cgcctggtgg cgtttatgca atatctgcga gtgctgttcg tcgcgggggc cgctgttctg
     5521 gtcacccgca tgatgctggg tgataacgct gaagcggtta accagcaaat cgtctggttc
     5581 ccgccagtga gcattaatct cctgcttacg attttgctcg cagtcgttgc cggtacggcg
     5641 ggatgcatgc tgcgtcttcc ttccggcacg atgctcatcc cgatgctggc gggcgcagtg
     5701 ctccagtctg gtcagctcat caccatcgaa ctaccggaat ggctgctggc gatggcgtat
     5761 atggcaattg gctggcggat tggtcttggt ttcgataaac aaatcttact gcgggcacta
     5821 cgcccgctac cgcaaatcct gctgtcgatt tttgctctgc tggctatttg tgcgggtatg
     5881 gcgtgggggc tgacccggtt tatgcacatt gattttatga ctgcctacct cgccaccagt
     5941 cccggcgggc ttgataccgt ggcggtcatc gccgcaggga gcaatgccga tatggcgctt
     6001 attatggcga tgcaaaccct gcgcctgttc agtattttgc tgacggggcc agccattgcg
     6061 cggtttattt caacctatgc gccgaagcgg tcggcctagt gctggcagcc tggacaccag
     6121 taaaacgggc gcgaggacaa cgtggttttc tcaatgatgc cgccacagcg ttcgcacagt
     6181 tcgccatcac gatgaaaaac cttaaagcga aacagcgccc catgatgctt attctcatcc
     6241 acctgccccc gcgtagcgta agaaaaccga ggaatctcca gtaacgcgtg tgcgagtgca
     6301 tccagttgcg ccgcattgag atctttcgct ttatgatttc cagtcaaccc aacctgccag
     6361 aggatctcca cccgcaaata attgccaagc ccagccagaa acgcctgatc gagcagtaat
     6421 cctgcaaact gacggttacg aaagcgcggt gacaataacc gttcctttac cacctccggc
     6481 gtcagattcg gatccagcac atcgggacca acgcgttgta aaaacggatg cgtggtcagt
     6541 tgttccggcc tcaacatctc aatatccgag gcgctataaa gcagaatggt tttgtcagcc
     6601 gcttgcagtt ttacccgcaa tactcgcgtg gtctgcggct cttcgccggt atcaaccacg
     6661 cgccagacgc cgtaaagctg attatggctg tagagcgtta agtcgttgga aaaatgagtt
     6721 aacaacgcct taccacgcgt ttccacatgg gtaacgtgtt gaccgataag ttgtgattga
     6781 taagttttta actgcgggaa ggcaaaccag acatcagtta gtggtttgcc tttgatcgcc
     6841 gcctccaggt tatccgctgc acggcggatc tccgggcctt caggcatggt gatatccttt
     6901 tttcattctt tattaatcgg ggttatgctg cgacaacaat ctccttttcg gcaaatgtag
     6961 agcgtagtcg gcgggcgaac gccagtgcgt gctcgccgtc gccatgcagg cagactgttt
     7021 gcgccgtgac cgttgcccat tcgccggtga tacttttgac tctgccgtgt tgcaccatct
     7081 ctagcgtttg cgccagcgcc tgttcttcgt tttcaatcaa cgcgcctggc tgacttcgcg
     7141 gcaccagcga gccgtcagcc tgataaccgc gatcggcaaa cacttcctcg cgcgttgtca
     7201 ggccatattg cttgcctgca cgaatcagct cgcttcctgc cagcccaacg agaatcaatg
     7261 ccggatcgca agcgtatacc gctctggcga tggcgtctgc cagttgtgct tctttcgccg
     7321 cctggttgta caacatgccg tgcggtttga catgacgcat tacgccgcct tgcgcacggg
     7381 caatcgttgc cagcgcgcca atttgataca gcgtctgggc gtaaacggtt tctggcggca
     7441 actgcatggc gctgcgacca aaattttccc tgtcgggaaa actcgggtga gcgccaattg
     7501 caacaccatt ttttattgct tcacgcacgc aagcctgcat ggtttgcgca tcgcctgcat
     7561 gaaatccaca ggcaatattg gcagaggaaa ccagcgttaa tagctctgcg tcgctggcgc
     7621 agccttcgcc cagatcggcg ttcaggtcaa ttttcattgt gcagccgcca cgctaattgt
     7681 tcaaaataac gttgctgatc ttgccgcgct ttcagtgcct cttccagtga acactggaca
     7741 aaatgaatcg gctgaccgag cggaatttgc gcaagatggt acatatcagc ctcaatgatg
     7801 caggcaatac gcgggtaacc accggtggtc tgtgcgtcgt tcatcaacac aatcggctgc
     7861 ccgttatgtg gcacctgcac cacgcccggt aacaaaccgt gagataacag ttcgcgatcg
     7921 gtggtgcgtt ttaaaatttg cccctgtaag cgatagccca tgcggttact ttgcgagcta
     7981 agctgccagg gcgaacgcca gaatgcatcc tgcgaggcac gatcgaactc atgatattcc
     8041 ggccccggca aggcgcgaat gcggttgccc cacagcagct gtttaacgcc ctgcgcttcc
     8101 ataaaatcac gcttcgcttt gccaatcggg agtcggtcac catccttcag taaacggcct
     8161 tccagcccgc caatccccac tttgagatcg gtgctgcatg accccattac cggcggaaca
     8221 tcaataccac ccgcgaccgc cagataactg cgcatcccgt gctgcgggcg tttaagcgtt
     8281 aaacgctggc ctgctttcat cggcaatcgc cagccggtcc agacggcatt atcatccagc
     8341 cgcgcttcgc aaccggcacc cgtcagagca aaccacccat cagtttcgaa ctcaacagtt
     8401 aactgaccga gcgtgatctc cagcgcgggg gcattggcgt cattacccac cagtaggtta
     8461 gcaatgcgta acgcgggcat atccagtgcg ccgcagtggc tgatacccga ctggcgaaaa
     8521 ccgtgacgac cgccatcctg cacagtggta tacatgcccg cacgaataat cttcagcata
     8581 ctccctcctt ctgcggtaca aagcgcacgc tgtctcccgg acgtaataag atgggttcgt
     8641 cacgcgccgg atcaaacagg ctgagtgagg tatgaccaat caactgccag ccacccggcg
     8701 ttgccagcgg ataaacacca gtctgcggcc cgccgatccc gacagaacct gccggaacga
     8761 gtaagcgcgg ttcagcgcgc cgtggcgtgt gtagttgttc cggcaaactc ccgagatacg
     8821 ggaagcccgg ttgaaaacct aaaaaccaga ccacgtattc cacggaggag tgcaattcaa
     8881 caacctgttt ttcgctcaac ccgcaatgcg ccgcgaccac cgccaaatcc ggtccgcctg
     8941 caccaccgta aaccaccgga atttcaataa agcgagactc aggttccagg gcctcgctct
     9001 cctcccacca gcgttgcaaa cgctctatgg catccagcgc cagcgactca ggattacgca
     9061 aaatcaccgt aatattgttc atgccgggaa tggcttcaac cacattcggc atatccacca
     9121 gacgctgcgc cagtcgccag atccgtttct ggctagccag cgtcaccggc ggttccagtt
     9181 ccagcactac cgccgtttca cctatcagat aacaacgcgc tcgttgcact ttcgatccct
     9241 ctgattattc gttatgcagg attaggaata tcaataaagg tcacatcaag atcggtattt
     9301 tcattcagcc actcgctcaa tgcgcgaata ccaccacgtt cagtggcatg gtgacctgca
     9361 gcataaaaat gcaatccctg ctcgcgggct gaatgaatgg tctgttcaga aacttcgcca
     9421 gtaataaaag catccacgcc aaaacgcgcg gcgctatcga taaaactttg cccgccgccc
     9481 gtgcaccagg cgacgcgctg gaccacctca ggtccggtat cgccacacca taacggctta
     9541 cgtcccagac gcgcttcaat ccaggaggct agttccagtc ccggcacagg catggtcagc
     9601 tctccccacg gcaccaacgg ctcaatttcg cccatgaccg tgatccccag taacgccgcc
     9661 agctgtgcgt tattgcccag ctcaggatgt gcgtcaagcg gcaaatgcca gccatacagg
     9721 ttgatatcat tcgccagcaa cgtttttaaa cggttacgct tcattccgcg aatgaccgga
     9781 gactctcctt tccagaagta gccatgatgc acaatgaccg catccgcgac cagacgcact
     9841 gcctcatcga gcaaagcctg gctggcggtg acaccggtaa caattttttg caccgtctct
     9901 ttgccttcca cctgcaaacc attcggcgcg taatcactaa tcgccgcgct gttcagtttt
     9961 tcgttaatca gttgttccag ttcggtgttt ttcatcttcg cgccttccgt attctgatag
    10021 ctacaccgtt aacatagcgt tacctcgcct gttcgtcgat agccaatctt ttgacatatt
    10081 aagaaacaaa cccgacgcca aaagacgcaa attgtttata tcatcacgct tcagctaatg
    10141 tatatttatg caatcagaca gcatattgca taaataatca gatctgtatc aaagttttaa
    10201 ctttaaggaa acacaagcac gccgcgcaac tattacgtga cgttgccgtc aattgtggaa
    10261 ttattatgaa taaacacgca tcacaaccgc gcgctattta ctatgtcgtt gcgctgcaaa
    10321 tctgggaata ttttagcttt tacggcatgc gtgccctgct gattctctat ctcaccaatc
    10381 aactaaaata caacgatact cacgcctacg agttatttag cgcctactgt tcgctggtgt
    10441 atgtcacgcc aatcctcggt ggctttttgg cggataaagt tctcggcaat cgcatggcgg
    10501 tgatgctggg ggcgttgttg atggcgatcg gtcatgtggt gctgggtgcc agtgagatcc
    10561 atccgtcatt cctctatctg tccctggcga ttatcgtttg tggctatggt ctgtttaaat
    10621 ccaatgtcag ttgcctgctc ggcgagctgt atgagccaac cgatccgcgt cgtgatggcg
    10681 gtttctcgct gatgtatgca gcgggtaacg tggggtctat tatcgcacct atcgcctgtg
    10741 gttacgccca ggaagagtac agctgggcga tgggctttgg cctggcggcg gtgggtatga
    10801 tcgcgggtct ggtcattttc ttatgtggca atcgtcattt cacacatacc cgcggcgtta
    10861 ataaaaaagt actgcgtgca acaaactttc tcctgcctaa ctggggatgg ctgctggttc
    10921 tgctggtggc aacgcctgcg ctgattaccg tactgttctg gaaagagtgg tcggtatacg
    10981 ccttaattgt cgcgactatt attggcctgg gtgtactggc aaaaatttat cgcaaagcag
    11041 aaaaccagaa acagcgtaag gagctggggc tgattgtgac gctcaccttc ttcagtatgt
    11101 tgttctgggc cttcgcacaa cagggcggca gctcgattag cctttatatc gaccgcttcg
    11161 ttaaccgcga tatgtttggt tataccgttc cgactgctat gttccagtcg attaatgcct
    11221 tcgcagttat gctgtgcggt gtgttcctgg cgtgggtggt taaagaaagc gtcgcaggta
    11281 atcgtaccgt gcgcatctgg ggaaaatttg ctcttggtct cggcctgatg agcgccgggt
    11341 tctgcattct gaccttaagc gcccgctggt ccgcaatgta tggtcactct tctctgccac
    11401 tgatggtatt aggcctggcg gtgatgggct ttgcggaact gtttatcgac ccggttgcca
    11461 tgtcgcaaat tacgcgcatt gaaattccgg gcgtgaccgg cgtattaacc ggcatctaca
    11521 tgctgctttc tggcgcgatc gcgaactatc tggcgggcgt gattgccgat cagacatcgc
    11581 aggcttcgtt tgatgcttcc ggggcgatca actactccat caatgcatat attgaagtat
    11641 ttgatcaaat tacctggggc gcactggcgt gtgtaggact ggtactgatg atttggctgt
    11701 atcaggcgct gaaattcaga aaccgcgcgc tggcgctgga gtcataatag gccagcacca
    11761 tacctgataa aacgctcgct gcgttttatc aggctccttt cctcgcttct tcgtacgctg
    11821 ccagcgttcg cagccttgcc tctttgtgat caactatcgg ttgcggataa tccagcttca
    11881 cacctgcttt ctgcgcccac ttccacggct catgcaccgc tttccctggt acatcgcgca
    11941 gctccggtaa ccatcgacga ataaactcgc cctcacggtc aaatttctcg ccctgagtta
    12001 tcggattgaa aatacgaaaa tacggcgcag catcagtacc cgtagaagcc gcccactgcc
    12061 agccaccgtt attagctgcc aaatcaccat caatcagctg cgacatgaaa tatcgctcgc
    12121 cttcgcgcca gtcgatcaac aaatctttca ccagaaaact ggctgtaatc atccgtagcc
    12181 ggttatgcat ccagccagtg ctgttaagct gacgcatagc ggcatcaaca atcgggtatc
    12241 ccgttttgcc tttctgccag gcctgtaaat gtgcgggatt gctttgccac tgcacacgat
    12301 ccgtccaggc aataaatgga cgatgtttac acaacgaggg gtaatacgtc atcagatgac
    12361 ggtagaactc gcgccagatc agctcactaa gccagacact accggcccca ccgtccagcg
    12421 cctgcggctg ttccgccaac aagcgatgca aacactggcg aggcgataac ccgcccgttg
    12481 ccaggctggc ggacaaacga ctggtgcctt ccactgccgg aaaatctcgt tgttgctcat
    12541 attctccggc accgttctgg caaaactggc gtaattgcgc aatcgccgct ttttcttcca
    12601 ccgggaaatg cgcagtatcg aaagactgac gaggataatt cagcgtaatg gatggcgcgg
    12661 gctctatcga tccgctacta cgaacttttg gcgcagcgac acactccggc atcccttccc
    12721 gcagccgttt cagccaggca ttcttaaaag gcgtaaagac tttgtacatc tcgtgattac
    12781 cggtcatcac cgcgccaggc ggcaggatca cgctgtcatc aaatccttca cacaccacgt
    12841 tacgcagcgc tctttcaact tccacatccc gcgcccgctc attcacttca tactgatagt
    12901 tataaaacag gtgggtaacg ctgttttccg cgcacacctg tttaactatt tcgacactgg
    12961 cgacaaagtc atccacttca cggaacaata aaggaatacc tttttccgca agcgctattt
    13021 gtagcccatt cagttgagca ttgataagtt cagcctgacg cggcgacatg ttatgcgtcg
    13081 cccactggcg tggtgtagcg atatataacg ccagcacgcg tgcagacgaa ttgcggcagg
    13141 cggcagccag tgcgagatta tcgtgcagac gtaaatcctg gcgaaaccag accagatggg
    13201 taatcataaa actcctgaat ggcgcaagtt aatcacacgc ggccaaagtt cgaaataacg
    13261 ctgccctgaa agccaggcgt caggataaag cgccatatag tgtttgatac gcatcagcgg
    13321 cgcaagaagt ggttgctcgc cacggcgata actgtcgata agcgccgcca actgctggcg
    13381 ttctgtggaa tcaatatgcg ggcgaaaata accctgaacg tgcatcaaaa cgttggtgtg
    13441 atcgcgcggg ttggcgggat gagaaagcaa aacaattacg cgttggtagt actggttgta
    13501 aaaatcatcc agattttgcc actggtgaat atcagccacg aacgggccaa gtttgcggta
    13561 ctccggctga gaatgcgcca gaaagacgag tttatagcgg ctgtgataat cgagaagcgc
    13621 cccgcgcgtt aagccatgtt ctttcagtcg atgtaactca tgaagggcga agatacggat
    13681 tagggtgctg tcatcaaatc gttgctgatt cattactgct cctgaacggt ttacaggagt
    13741 aagtgtagac gttgagaaat gatatttgtg aagttaatcc tgttgtagca ggacaaattt
    13801 acgaaagcct gctccccgca aggactgacg tcaggtagtt tctaaccata gctgcccttt
    13861 ctattcaaac gtgaaatcaa taagctcatc agggacatcg caaacaatat gaagacgggt
    13921 ttcttctctg ccgtgactct tttcactaat tgcgtaactg tcatgcttgg gattatttaa
    13981 ttctttcagc ggaaattttt cctcgaaggc tttatttagc cgcccctggt ttccttttac
    14041 agcgaataaa taatcacctc cctgtttttg tatcttctct gcaatatctt tctggcaacc
    14101 catcgcatcg gttgtgatga tttttccttt aatatccagc atgttaagaa gttctggaat
    14161 agctgtgatc tcattggatt tcttatccgt cttgatctgc ccgatgacca gactgtgcat
    14221 tgttgagaac gcactaatga catgaatcgc tcccctgcgg cgactcttgt cataagagtg
    14281 ccggagcgtt tttccatcaa ttgcaatgac gtctttatca tttgatgaat ggcaatcacg
    14341 catccagtta ataaagcact c
//
LOCUS       DAPHDW010000114        14328 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.115, whole genome shotgun sequence.
ACCESSION   DAPHDW010000114 DAPHDW010000000
VERSION     DAPHDW010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.115"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            12..542
                     /locus_tag="RH057_003367"
     CDS             12..542
                     /locus_tag="RH057_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416483.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV3129689.1"
                     /translation="MNRFSKTQIYLHWITLLFVAITYAAMELRGWFPKGSSTYLLMRE
                     THYNAGIFVWVLMFSRLIIKHRYSDPSIVPPPPAWQMKAASLMHIMLYITFLALPLLG
                     IALMAYSGKSWSFLGFNVSPFVTPNSEIKALIKNIHETWANIGYFLIAAHAGAALFHH
                     YIQKDNTLLRMMPRRK"
     gene            complement(1112..1201)
                     /locus_tag="RH057_003368"
     tRNA            complement(1112..1201)
                     /locus_tag="RH057_003368"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1165..1167),aa:Ser,seq:cga)
     gene            1295..2092
                     /gene="mtfA"
                     /locus_tag="RH057_003369"
     CDS             1295..2092
                     /gene="mtfA"
                     /locus_tag="RH057_003369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HDV3129690.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVTLAE
                     RFLQQKRLVPLQGFELDSLRTCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFIPLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANNTDSFSATNVH"
     gene            2193..2268
                     /locus_tag="RH057_003370"
     tRNA            2193..2268
                     /locus_tag="RH057_003370"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2226..2228,aa:Asn,seq:gtt)
     gene            2580..9656
                     /gene="yeeJ"
                     /locus_tag="RH057_003371"
     CDS             2580..9656
                     /gene="yeeJ"
                     /locus_tag="RH057_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter adhesin YeeJ"
                     /protein_id="HDV3129691.1"
                     /translation="MATKKRSGEEINDRQILCGMGIKLCRLTAGICLVTQLVFPMAAA
                     AQGVVNAATQQPVPAQIAIANANTVPYTLGALESAQSVAERFGISVAELRKLNQFRTF
                     ARGFDNVRQGDELDVPAQVSEKKLTPPPGNSSDNLEQQIASTSQQIGSLLAEDMNSEQ
                     AANMARGWASSQASGAMTDWLSRFGTARITLGVDEDFSLKNSQFDFLHPWYETPDNLF
                     FSQHTLHRTDERTQINNGLGWRHFTPTWMSGINFFFDHDLSRYHSRAGIGAEYWRDYL
                     KLSSNGYLRLTNWRSAPELDNDYEARPANGWDVRAESWLPAWPHLGGKLVYEQYYGDE
                     VALFDKDDRQSNPHAITAGLNYTPFPLMTFSAEQRQGKQGENDTRFAVDFTWQPGSAM
                     QKQLDPNEVAARRSLAGSRYDLVDRNNNIVLEYRKKELVRLTLTDPVTGKSGEVKSLV
                     SSLQTKYALKGYNVEATALEAAGGKVVTTGKDILVTLPAYRFTSTPETDNTWPIEVTA
                     EDAKGNLSNREQSMVVVQAPTLSQKDSSVSLSTQTLNADSHSTATLTFIAHDAAGNPV
                     VGLVLSTRHEGVQDITLSDWKDNGDGSYTQILTTGAMSGTLTLMPQLNGVDAAKAPAV
                     VNIISVSSSRTHSSIKIDKDRYLSGNPIEVTVELRDENDKPVKEQKQQLNNAVSIDNV
                     KPGVTTDWKETADGVYKATYTAYTKGSGLTAKLLMQNWNEDLHTAGFIIDANPQSAKI
                     ATLSASNNGVLANENAANTVSVNVADEGSNPINDHTVTFAVLSGSATSFNNQNTAKTD
                     VNGLATFDLKSSKQEDNTVEVTLENGVKQTLIVSFVGDSSTAQVDLQKSKNEVVADGN
                     DSVTMTATVRDAKGNLLNDVMVTFNVNSAEAKLSQTEVNSHDGIATATLTSLKNGDYR
                     VTASVSSGSQANQQVNFIGDQSTAALTLSVPSGDITVTNTAPQYMTATLQDKNGNPLK
                     DKEITFSVPNDVASKFSISNGGKGMTDSNGVAIASLTGTLAGTHMIMARLANSNVSDA
                     QPMTFVADKDRAVVVLQTSKAEIIGNGVDETTLTATVKDPSNHPVAGITVNFTMPQDV
                     AANFTLENNGIAITQANGEAHVTLKGKKAGTHTVTATLGNNNTSDSQPVTFVADKASA
                     QVVLQISKDEITGNGVDSATLTATVKDQFDNEVNNLPVTFSSASSGLTLTPGVSNTNE
                     SGIAQATLAGVAFGEKTVTASLANNGASDNKTVHFIGDTAAAKIIELTPVPDSIIAGT
                     PQNSSGSVITATVVDNNGFPVKGVTVNFTSNAATAEMTNGGQAVTNEQGKATVTYTNT
                     RSSIESGARPDTVEASLENGSSTLSTSINVNADASTAHLTLLQALFDTVSAGETTSLY
                     IEVKDNYGNGVPQQEVTLSVSPSEGVTPSNNAIYTTNHDGNFYASFTATKAGVYQLTA
                     TLENGDSMQQTVTYVPNVANAEITLAASKDPVIADNNDLTTLTATVADTEGNAIANTE
                     VTFTLPEDVKANFTLSDGGKVITDAEGKAKVTLKGTKAGAHTVTASMTGGKSEQLVVN
                     FIADTLTAQVNLNVTEDNFIANNVGMTRLQATVTDGNGNPLANEAVTFTLPADVSASF
                     TLGQGGSAITDINGKAEVTLSGTKSGTYPVTVSVNNYGVSDTKQVTLIADAGTAKLAS
                     LTSVYSFVVSTTEGATMTASVTDANGNPVEGIKVNFRGTSVTLSSTSVETDDRGFAEI
                     LVTSTEVGLKTVSASLTDKPTEVISRLLNASADVNSATITSLEIPEGQVMVAQDVAVK
                     AHVNDQFGNPVAHQPVTFSAEPSSQMIISQNTVSTNTQGVAEVTMTPERNGSYMVKAS
                     LPNGASLEKQLEAIDEKLTLTASSPLIGVYAPTGATLTATLTSANGTPVEGQVINFSV
                     TPEGATLSGGKVRTNSSGQAPVVLTSNKVGTYTVTASFHNGVTIQTQTTVKVTGNSST
                     AHVASFIADPSTIAATNTDLSTLKATVEDGSGNLIEGLTVYFALKSGSATLTSLTAVT
                     DQNGIATTSVKGAMTGSVTVSAVTTAGGMQTVDITLVAGPADTSQSVLKSNRSSLKGD
                     YTDSAELRLVLHDISGNPIKVSEGMEFVQSGTNVPYIKISAIDYSLNINGDYKATVTG
                     GGEGIATLIPVLNGVHQAGLSTTIQFTRAEDKIMSGTVSVNGTDLPTTTFPSQGFTGA
                     YYQLNNDNFAPGKTAADYEFSSSASWVDVDATGKVTFKNVGSNSERITATPKSGGPSY
                     VYEIRVKSWWVNAGEAFMIYSLAENFCSSNGYTLPRANYLNHCSSRGIGSLYSEWGDM
                     GHYTTDAGFQSNMYWSSSPANSSEQYVVSLATGDQSVFEKLGFAYATCYKNL"
     gene            complement(9918..10970)
                     /locus_tag="RH057_003372"
                     /pseudo
     CDS             complement(9918..10970)
                     /locus_tag="RH057_003372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310804.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
     gene            11285..12601
                     /gene="shiA"
                     /locus_tag="RH057_003373"
     CDS             11285..12601
                     /gene="shiA"
                     /locus_tag="RH057_003373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate transporter"
                     /protein_id="HDV3129692.1"
                     /translation="MDSTLISTRPDEGTLSLSRARRAALGSFAGAVVDWYDFLLYGIT
                     AALVFNREFFPQVSPAMGTLAAFATFGVGFLFRPLGGVIFGHFGDRLGRKRMLMLTVW
                     MMGIATALIGILPSFSTIGWWAPILLVTLRAIQGFAVGGEWGGAALLSVESAPKNKKA
                     FYSSGVQVGYGVGLLLSTGLVSLISMMTTDEQFLSWGWRIPFLFSIVLVLGALWVRNG
                     MEESAEFEQQQHYQAAAKKRIPVIEALLRHPGAFLKIIALRLCELLTMYIVTAFALNY
                     STQNMGLPRELFLNIGLLVGGLSCLTIPCFAWLADRFGRRRVYITGTLIGTLSAFPFF
                     MALEAQSIFWIVFFSIMLANIAHDMVVCVQQPMFTEMFGASYRYSGAGVGYQVASVVG
                     GGFTPFIAAALITYFAGNWHSVAIYLLAGCLISAMTALLMKDSQRA"
     gene            12703..14157
                     /gene="amn"
                     /locus_tag="RH057_003374"
     CDS             12703..14157
                     /gene="amn"
                     /locus_tag="RH057_003374"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HDV3129693.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGNYITSGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSAGLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDVWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
BASE COUNT         3858 a         3428 c         3375 g         3667 t
ORIGIN      
        1 gagctacaaa aatgaaccga ttctcaaaaa ctcaaattta tttacattgg ataacgctgc
       61 ttttcgttgc aataacctat gccgcgatgg aactccgtgg ctggtttcct aaaggtagta
      121 gtacttatct gctgatgcga gaaacacatt acaatgcggg tatattcgtt tgggtgttaa
      181 tgttttcacg cctgattata aaacaccgtt atagtgatcc ttctattgtg ccaccgccac
      241 ctgcctggca aatgaaagcg gcttcgctaa tgcacatcat gctttatata accttccttg
      301 cacttcctct gctggggatt gctttgatgg cttacagtgg aaaatcgtgg agtttccttg
      361 gtttcaatgt gtctcccttt gttaccccaa acagcgaaat taaagcactg ataaaaaata
      421 ttcacgaaac ctgggcaaat ataggctact ttttaatcgc agctcacgct ggcgcagcac
      481 tctttcatca ctacattcag aaagataata ctctgttacg aatgatgcct cgccgcaaat
      541 aagtcgatcc ccttacaagg aaatgtgaac catggacatc aacgcgtcat aaatgtttac
      601 gcaagtgcac aatgcaccct ttccactatc aacaaggagg agcagaaact gtgtaaatcg
      661 tacaatggta agacttatca aaaatacagg ttaattttgg tatttaaacc ttttttactt
      721 gttagtatga tacaaaggct ttcaaaaaag ctgcgaaatt caacaactct tggaaattat
      781 ggactttgtc ccgcggcttt aatctgacgg ccgcgttcct tttttgactc aattattgac
      841 cccttctcta cgcaacttca gttcccacca ccaactttgc ggcagtttca taggatgaat
      901 gtctaaaaga ataatggtga gcgataagaa aacgactgaa taactgcaca ttttcgctcg
      961 aaagcttcct gtcagatcca tagcgaatca agtgctgaat gtcacagtat cgaacagaaa
     1021 acagtgacga tctaaccctt caagaatatt ctacgattgt tctgtttagg aaaagcaagg
     1081 cgggaagtcg ggagataagt cattgataaa gtggcggaga gagggggatt tgaacccccg
     1141 gtagagttgc ccctactccg gttttcgaga ccggtccgtt cagccgctcc ggcatctctc
     1201 cgttcagatg gttgccatga tgccaggaaa tttggcattt taacagtccc tgtccgtgca
     1261 attttgttca agtgacgagt ttgcgagcaa aacgatgatt aagtggccct ggaaagtaca
     1321 agaatcagca catcaaactg cccttccctg gcaggaagca ctatcgatcc cccttttaac
     1381 gtgtctgacg gaacaggaac aaagcaaatt agtcactctt gccgaacgtt ttttacagca
     1441 aaagcggctt gttcctttac agggctttga actggattca ttaagaacct gccggatagc
     1501 acttctattt tgcctacccg ttctggaatt aggactggaa tggctggatg gttttcatga
     1561 agtcttaatt tatcctgcgc catttgtggt cgatgatgaa tgggaagacg atatcggtct
     1621 ggtgcataac caacgtattg ttcagtcagg tcagagctgg cagcaagggc ctatcgtttt
     1681 gaactggttg gatatacaag attcttttga tgcttctggt tttaacctga ttattcatga
     1741 agtcgctcat aagctggaca cccgtaacgg cgatcgcgcc agcggagttc cctttattcc
     1801 gttgcgtgag gttgctggct gggaacacga tcttcatgct gcaatgaaca acattcagga
     1861 agaaatcgaa ttggttggcg agaatgcggc gagcattgat gcttatgctg ccagtgatcc
     1921 tgctgaatgt tttgccgtac tttctgaata tttctttagc gccccagaac tttttgctcc
     1981 tcgtttccct tcattgtggc aacgtttctg ccaattttat caacaagatc ctttgcagag
     2041 actgcatcac gctaataata cagactcgtt ttcggcgacg aatgttcatt aattaacaac
     2101 tttgcagatt aattaaccaa ttgaaatgac ttatgaaatt tagtgttgac agacaaggta
     2161 ccgctaagta atatgcgccc cgttcacacg attcctctgt agttcagtcg gtagaacggc
     2221 ggactgttaa tccgtatgtc actggttcga gtccagtcag aggagccaaa ttctaaaaat
     2281 tcgcttctta gtgcaatgtc actaacctta gttgaacatt gttttttaac ggatagcggg
     2341 tttttaacat cttaagcgcc ctcgaacttt atggttgagg gcgttttgct atggcgtcat
     2401 caccattttc ccctcgatta taaaacttga gtaattcagt agtctcccct cttgcaactc
     2461 acacccaaaa ctgcctaacg aaaagttatt aattttcaat catattgcta tcaggattta
     2521 cattttttcg ctgtgctaga aagggcgcat ttatgttagc tcgttcaggg aaggtaagca
     2581 tggctacgaa gaagagaagt ggagaagaaa taaatgaccg acaaatctta tgcgggatgg
     2641 gtatcaaact atgccgctta actgcgggta tctgcctggt aactcaactt gtgttcccta
     2701 tggctgcggc agcacaaggt gtggtaaacg ccgcaaccca acaaccagtt cctgcacaaa
     2761 ttgccattgc aaatgccaat acggtgccct acacccttgg agcgctggaa tcggcccaaa
     2821 gcgttgccga acgtttcggt atttcggtgg ctgagttacg caaactcaac cagtttcgta
     2881 cgtttgctcg aggttttgat aatgtccgcc agggtgatga actggatgtc ccggcacaag
     2941 ttagtgaaaa aaaattaacc ccgccgccgg gtaatagcag tgacaacctc gagcaacaga
     3001 tagccagtac ttcacagcaa atcgggtctc tgctcgccga agatatgaac agcgagcaag
     3061 cggcaaatat ggcgcgtgga tgggcctctt ctcaggcttc aggcgcaatg acagactggt
     3121 taagccgctt cggtaccgca agaatcacgc tgggcgtgga tgaagatttt agcctgaaga
     3181 actcccagtt cgattttctc catccgtggt atgaaacgcc tgataatctc tttttcagtc
     3241 agcatactct ccatcgtact gacgagcgta cgcagattaa caacggctta ggttggcgtc
     3301 atttcactcc cacatggatg tcgggcatca acttcttttt cgaccacgat cttagccgtt
     3361 accactcccg cgccggcatt ggcgcggagt actggcgcga ctatctaaaa ttaagcagta
     3421 acggctattt gcgactgacc aactggcgca gcgcacctga actggacaac gattatgaag
     3481 cacgcccggc caatggctgg gatgtacgcg cagaaagctg gctacccgcc tggccgcacc
     3541 ttggcggtaa actggtctat gaacagtatt atggcgatga agtggccctg ttcgataaag
     3601 acgatcggca aagtaatcct catgccataa ccgctggact taactatacc cccttcccgc
     3661 tgatgacctt cagcgcggag caacgccagg gtaaacaggg cgaaaatgac acccgttttg
     3721 ccgtcgattt tacctggcaa cctggcagcg caatgcagaa acagcttgac ccgaatgaag
     3781 tcgctgcacg gcgtagcctt gcaggcagcc gttatgatct ggtggatcgc aacaacaata
     3841 tcgttctgga atatcgcaaa aaagaactgg ttcgcctgac cctgacagac cccgtgacag
     3901 ggaagtcagg agaagtgaaa tcactggttt cgtcgctaca aaccaaatat gccctgaaag
     3961 gctataacgt cgaagccacc gcactggaag ctgccggtgg caaagtggtc acaacgggta
     4021 aagatattct ggttaccctg ccggcttacc ggttcaccag tacgccagaa accgataaca
     4081 cctggccgat tgaagtcacc gccgaagatg ccaaaggcaa tttgtcgaat cgtgaacaga
     4141 gcatggtggt cgttcaggca cctacgctaa gccagaaaga ttcctcggta tcgttaagta
     4201 cccaaacatt gaacgcggat tcccattcaa ccgccacact gacttttatt gcgcatgatg
     4261 cagcaggtaa tcctgttgtc gggctggtgc tctcgacgcg tcacgaaggt gttcaggaca
     4321 tcaccctttc tgactggaaa gataatggtg acggaagcta tacccagatc ctgaccacag
     4381 gagcgatgtc tggcacgctg acgctgatgc cacagctgaa tggtgtggat gcggctaaag
     4441 cccccgccgt ggtgaatatc atttctgttt cgtcatcccg aactcactcg tcaattaaga
     4501 ttgataagga ccgttatctc tccggcaatc ctatcgaggt gacggtagaa ctgagagatg
     4561 aaaatgacaa acctgttaag gaacaaaaac agcaactgaa taacgcagtc agcatcgaca
     4621 acgtgaaacc aggagtcact acagactgga aagaaaccgc agatggcgtc tataaggcga
     4681 cctataccgc ctataccaaa ggcagtggac ttactgcgaa gctattaatg caaaactgga
     4741 atgaagattt gcataccgct ggatttatca tcgacgccaa cccgcagtca gcgaaaattg
     4801 cgacattatc tgccagcaat aatggtgtgc tcgccaatga gaatgcagca aacaccgtct
     4861 cggtcaatgt cgctgatgaa ggaagcaacc caatcaatga tcataccgtc acgtttgcgg
     4921 tattaagcgg atcggcaact tccttcaaca atcaaaacac cgcaaaaacg gatgttaatg
     4981 gtctggcgac ttttgatctg aaaagtagta agcaggaaga caacacggtt gaagtcaccc
     5041 ttgaaaatgg cgtgaaacaa acgttaatcg tcagttttgt cggcgactcg agtaccgcgc
     5101 aggttgatct gcagaagtcg aaaaatgaag tggttgctga cggcaatgac agcgtcacaa
     5161 tgaccgcgac cgtccgggat gcaaaaggca acctgctcaa tgacgtcatg gtcactttca
     5221 atgttaattc agcagaggcg aaactgagcc aaaccgaagt gaatagccac gacgggatcg
     5281 ccacagctac gctgaccagt ttgaaaaatg gtgattatag ggttacggcc tctgtgagct
     5341 ctggttccca ggctaatcaa caggtgaatt ttatcggtga tcaaagtact gctgccctga
     5401 ccctcagtgt gccttcaggt gatatcaccg tcaccaacac agctccgcaa tatatgactg
     5461 caaccttgca ggataaaaat ggcaacccac taaaagataa agaaatcacc ttctctgtgc
     5521 caaacgacgt cgcaagtaag ttctcgatta gcaacggagg aaaaggcatg acggatagta
     5581 acggggttgc aatcgcctcc ctgaccggca cgttagcggg cacgcatatg atcatggctc
     5641 gtctggctaa cagcaatgtc agcgatgcac agccaatgac gtttgtggcg gataaagaca
     5701 gagcggttgt cgttttgcaa acatcgaaag cggaaatcat tgggaatggc gtggatgaga
     5761 caactctgac agcaacagtg aaagatccat cgaatcatcc ggtggcgggg ataacggtaa
     5821 acttcaccat gccacaggac gttgcggcaa actttaccct tgaaaataac ggtattgcca
     5881 tcactcaggc caatggggaa gcgcatgtca cgctgaaagg taaaaaagcg ggcacgcata
     5941 cggttaccgc aacgctgggt aataacaata ccagtgattc gcagccggta acatttgtgg
     6001 cggacaaagc ctcggctcag gttgtcctgc agatatcaaa agatgagatc acaggtaatg
     6061 gcgtcgatag cgcaacgcta actgcaacgg ttaaagatca gttcgacaat gaggtgaata
     6121 atcttccggt aacattcagc tcagcctctt caggactcac cctgaccccg ggagtaagta
     6181 ataccaacga gtctggcatc gcgcaggcca ctctcgcagg cgttgccttt ggtgagaaga
     6241 cggttactgc atcactggct aataatggtg ccagcgacaa caaaactgtg cattttattg
     6301 gcgacacagc ggcggcaaaa attatcgagt tgacgcctgt cccagacagc ataatcgccg
     6361 gtaccccgca gaacagctcc ggcagcgtca tcaccgccac agtcgttgat aataatggct
     6421 ttccggtgaa aggtgtgact gtgaacttca ccagcaacgc agcgacagcc gaaatgacga
     6481 acggcggtca agccgtgacg aacgaacagg gtaaggctac cgtcacttat accaataccc
     6541 gctcctcgat agaatcagga gcgagaccgg ataccgttga ggccagtctg gaaaatggta
     6601 gctccacgct tagcacatca attaatgtca acgctgatgc gtctacggca catctcacct
     6661 tgctacaggc actttttgat acagtctccg caggcgagac aaccagtctg tatattgagg
     6721 tgaaggataa ttacggcaac ggtgtccccc agcaggaggt aaccctcagc gtttcaccaa
     6781 gtgaaggcgt gacccccagt aataacgcta tatatactac caaccacgac ggcaattttt
     6841 acgcaagctt taccgctaca aaagccgggg tttatcaatt gacggcaacc ctcgaaaatg
     6901 gcgattcgat gcaacaaaca gtgacctatg tgccgaacgt cgcgaatgct gaaatcacgc
     6961 tggcagcctc gaaggatccg gtgattgccg acaataacga tctcacgaca ctaacagcaa
     7021 cagtcgctga tacagagggc aatgcgatag ccaacactga ggtaacattt actctgccgg
     7081 aagatgtgaa ggcgaacttc acgctgagcg atggcggtaa agtgattact gatgctgaag
     7141 gcaaagcgaa agtcacgctg aaaggtacaa aagcaggcgc tcatactgtt acagcatcga
     7201 tgactggcgg taagagtgag cagttggtgg tgaactttat tgcggatacg ctcactgcac
     7261 aggttaatct taacgttacc gaggacaatt ttatcgctaa taacgtcggg atgaccaggc
     7321 tgcaggcaac agtgactgat ggaaacggca acccgttagc caatgaggcg gtgacattca
     7381 cgctaccggc agatgtgagc gcaagcttta ctctcggaca aggcggttcc gccattactg
     7441 atatcaacgg caaggctgaa gttacactga gcggtacaaa atccggcacc taccccgtga
     7501 cagttagcgt gaacaattat ggtgtcagtg atacgaaaca ggtgactttg attgccgatg
     7561 ctggtaccgc aaaactagcc tccttaacct ctgtatactc attcgtcgtc agcacgaccg
     7621 agggcgcaac catgacggca agcgtcactg acgctaacgg caacccggta gaaggcataa
     7681 aagttaattt ccgcggaacc tccgtcacgc taagcagcac cagcgttgaa acggatgatc
     7741 ggggtttcgc tgaaattctt gtgacaagca ccgaggtcgg actgaaaaca gtttcagcct
     7801 ctctgacaga taaacctact gaagtcatct cgcgattact gaatgccagt gcagatgtta
     7861 attctgcgac gattaccagt ctggagatac cggaaggtca ggtaatggtc gcacaagacg
     7921 tagcagttaa agctcacgtt aacgaccagt ttggcaaccc ggttgcgcat caacccgtga
     7981 cattcagtgc agagccatcc tcgcaaatga tcatcagcca gaatacggtc tctactaata
     8041 cgcagggtgt agccgaggtc accatgacgc ccgaaagaaa cggttcgtat atggtgaaag
     8101 catccctgcc gaatggagcc tcacttgaga aacaactgga ggctattgat gaaaaactga
     8161 cactcacggc gtccagtccg cttatcggtg tctatgcccc tacaggcgct actctgacgg
     8221 caacgctaac ctctgcaaat ggcactccag tggagggtca ggtcatcaac tttagcgtaa
     8281 cgccagaagg ggcgacgtta agtggcggaa aagtgagaac taactcttca ggtcaggctc
     8341 cagtcgtttt gaccagcaat aaagtcggta catatacggt gactgcatct ttccataacg
     8401 gcgtaacaat acagacacag acaaccgtga aagtcactgg caactcaagc accgcccatg
     8461 ttgctagctt tatcgctgat ccatcgacta tcgccgccac caacactgat ttaagtacct
     8521 taaaggcaac ggttgaggat ggcagtggta acctgatcga aggtctcact gtgtacttcg
     8581 ccttaaaaag cggctctgcc acattaacgt cattaacagc ggtgaccgat caaaacggaa
     8641 tcgcgacaac aagcgtgaaa ggagcgatga caggtagcgt cacggtaagc gcagtcacga
     8701 ccgctggtgg aatgcaaaca gtagatataa cgctggtggc tggcccggca gacacctcgc
     8761 agtccgtcct taagagcaat cggtcatcac tgaaagggga ctataccgat agtgctgaat
     8821 tacgtcttgt tctgcacgat atatcaggca atccgatcaa agtttctgaa gggatggaat
     8881 ttgtgcaatc aggtactaac gtgccctata taaaaattag cgcaattgat tacagtctaa
     8941 atatcaacgg tgattacaaa gccactgtta caggaggcgg agagggtatc gcaacgctga
     9001 tccctgtatt gaatggtgtt catcaagctg gtctgagtac cacaatacaa ttcactcgcg
     9061 cagaagacaa aataatgagc ggtacagtat cagtcaatgg tactgaccta ccgacaacta
     9121 cattcccttc gcaggggttc accggggcgt attatcagtt gaataatgac aactttgccc
     9181 caggaaaaac ggcggctgat tatgagtttt caagctctgc ctcctgggtc gatgttgatg
     9241 ctaccggtaa agtgacattt aaaaatgtcg gcagcaattc ggaaaggatt acggcgacgc
     9301 caaaatcagg aggccctagc tatgtatacg aaatccgtgt gaagagttgg tgggtgaacg
     9361 ccggcgaggc tttcatgata tacagccttg ctgaaaattt ttgcagcagc aatggctaca
     9421 cgctccccag agcaaactat ttaaaccact gtagttcccg aggcatcggg tcactgtaca
     9481 gtgaatgggg agatatgggg cattacacga ctgacgctgg ttttcaatca aatatgtatt
     9541 ggtcatctag tcccgcaaac tcaagcgaac aatacgtagt ttccctggca acaggtgatc
     9601 aaagcgtatt tgaaaagctt gggtttgctt atgcgacatg ttataaaaac ctgtgatttt
     9661 ccgttataat ttcttaaaga gtgttccatt cggggcactc ttttttacat tccctctaca
     9721 gaactagccc ttctctcaca gaaaatgaat aaataaaaat gcggcaccgc cagaatcgcg
     9781 ttcgatgtga ctatgatcat tttcggtgag tactgctttg atctaaaggc tggttttctg
     9841 tgatgattga ccagcctcag agtattgatg acaccaccgt tcataacaga tcggcattac
     9901 tatgcataag tactgtctca tattctcgcc aaaacacttt ttaatgcctt tgcgaattca
     9961 tccagactta tattgtcaat tttttgccca acgacgactg actccgagac atcggccatt
    10021 gcgggccatc gcgttttgta atcagcataa attgcaacaa caggggtatt ataggcactt
    10081 gccatatgta atattgaggt gtcaggagtt attgcgatat aagcatcttt tatgattgct
    10141 gctgaacgtt taatcgaggg tggtaaggat aaacggtaga cattattata acaatccact
    10201 aatgccctgg ctttatcttc acctttgggt ccatgaacga tcactattgg tatatctgtt
    10261 tcactttaaa tttttgctat tagattttct gcaatcgata atgaaaatgt acgttcctgc
    10321 gagctacctt ctaaattgaa cgcaatgtaa gatcctaacg atcgcatttc ctcgcgcacc
    10381 tcatcgagta catcctcact tagtggcaat tcatatattg gcctgactgc tggaaaaccc
    10441 gcctcacgca tcataaatgc ccatgtcata ggtacgggag cccggagttt ctgatccata
    10501 cgggacgcgt tcttgcacaa aggggagaag caattcatgg ttataccaac aacctgaaaa
    10561 ttcgtttttg ctttcaactg actgataaat aacatcgttt tcaggttctt tttacgcatc
    10621 ccctcaatgc aaagatccgg cgtaccgtat tgctgtgtta tgttctttgc taaatctttt
    10681 atttctttta atgttgcgtg atcctgcata gtcattgtga ctaatgttaa tttagtctgt
    10741 tcaagtttaa gcgcattaaa gacttctaaa ttaattgtcg acgttacaat taaaagatgc
    10801 ttaattttat gcaattcaag cgcccgaata acaggaaaga tggccatagc atcgccaatc
    10861 tgatcgggaa tatggatgac aacaaagtct gttttttcaa tattgaaatt ataagcttta
    10921 taatcgtagt aactaaatgc aatacgtctc aacaatgata ctaaaaacat acctaacctc
    10981 gcctccctac tggttataat gcaatgcagt ctatcagact catcagggtg ccattttgtg
    11041 catatgcgga cttttatgtt tcatatctct aacctgtggg tcctctgctt aatccttaaa
    11101 caacaccagc aactcctgcg ctttcatctt ccatcgaatt tttcatgttg ccgctaatca
    11161 gccataaaat catttgcaga tgcgctctgt cgaggtagtc tcataaggtt cgtttataga
    11221 tcgacggcaa tgtgagttac cttttccata ctaattataa aaagacagta caaacaggat
    11281 cattatggac tccacgctca tctccactcg tcccgatgaa gggacgcttt cgttaagtcg
    11341 cgcccgacga gctgcgttag gcagcttcgc tggtgccgtc gtcgactggt atgatttttt
    11401 actctatggc atcaccgccg cactggtgtt taatcgcgag tttttcccgc aagtaagccc
    11461 ggcgatggga acgctcgccg catttgctac ctttggcgtc ggatttcttt tccgtccgct
    11521 cggcggtgtc attttcggtc actttggcga ccgactggga cgtaagcgca tgttaatgct
    11581 gaccgtctgg atgatgggca tcgcgacagc cttgattggt attcttcctt cattctcgac
    11641 cattgggtgg tgggcaccta ttttgctggt gacactgcgt gccattcagg gatttgcagt
    11701 cggcggcgaa tggggaggcg cggcgttgct ttccgttgaa agtgcaccga aaaataaaaa
    11761 agccttttac agtagcggtg tacaagttgg ctacggtgta ggtttactgc tttcaaccgg
    11821 actggtttca ttgatcagta tgatgacgac tgacgaacag tttttaagct ggggctggcg
    11881 cattcctttc ctgtttagca tcgtactggt actgggagca ttgtgggtgc gcaatggcat
    11941 ggaggagtcc gcggaatttg aacaacagca acattatcaa gctgccgcga aaaaacgcat
    12001 cccggttatc gaagcgctgt tacgacatcc cggtgctttc ctgaagatta ttgcgctacg
    12061 actgtgcgaa ttgctgacga tgtacatcgt tactgccttt gcacttaatt attcaaccca
    12121 gaatatgggg ctaccgcgcg aacttttcct taatattggt ttgctggtag gtggattaag
    12181 ctgcctgaca attccctgtt ttgcctggct tgccgatcgt tttggtcgcc gcagggttta
    12241 tatcacaggt acgttaatcg gaacgttgag cgcatttcct ttctttatgg cgcttgaagc
    12301 acaatctatt ttctggatag ttttcttctc cataatgctg gcaaacattg cgcatgacat
    12361 ggtggtgtgt gtgcaacaac cgatgtttac cgaaatgttt ggtgccagtt atcgctatag
    12421 tggcgctgga gtcggttatc aggttgccag tgtggttggc ggtggattta caccttttat
    12481 tgccgctgca ctcatcactt actttgccgg gaactggcat agcgtcgcca tttatttgct
    12541 ggctggatgc ctgatttccg caatgaccgc tttgttgatg aaagacagtc aacgcgcttg
    12601 atagcctggc gaagatcatc cgatcttcgc cttacacttt tgtttcacat ttctgtgaca
    12661 tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa taagggctcc
    12721 ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca atctgtagtc
    12781 gcattacgca acgccattgg caactatatt acaagtggcg aattacctga tgaaaacgcc
    12841 cgcaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaacc
    12901 aatcccccca aaacgcgcgc atttggtcgc tttacccacg caggcagcta caccaccacg
    12961 attactcgcc ctactctctt tcgttcgtat cttaatgaac aacttacgtt gctgtatcag
    13021 gattatggtg cgcatatctc agtgcaaccc tcgcagcatg aaatccctta tccttatgtc
    13081 atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgggttaac tcgctacttc
    13141 ccgacaacag aactggcgca aattggcgat gaaactgcag acggcattta tcatccaact
    13201 gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg
    13261 cgccattata ccggtacgcc agttgaacat tttcagccgt tcgtcttgtt taccaactac
    13321 acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt
    13381 ccctacattg ccctttcttg tgctggcggg aactggatca ccgccgaaac cgaagcgcca
    13441 gaagaagcca tttccgacct tgcatggaaa aaacatcaga tgccagcatg gcatttaatt
    13501 accgccgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc aaatgctaaa
    13561 accatctgcg atcatctggc agtgctacgc ccggatgtct ggttgatgat tggtcactgt
    13621 ggcggattac gtgaaagtca ggccattggc gattatgtac ttgcacacgc ttatttacgc
    13681 gatgaccacg ttcttgatgc ggttctgccg cccgatattc ctattccgag cattgctgaa
    13741 gtgcaacgtg cgctttatga cgccaccaag ctggtgagcg gcaggcccgg tgaggaagtc
    13801 aaacagcggc tacgtactgg tactgtggta accacagatg acaggaactg ggaattacgt
    13861 tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt
    13921 gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt
    13981 tcagataaac cgttgcatgg cgagattaaa cttcccggcc aggctaaccg tttttatgaa
    14041 ggcgctattt ccgaacatct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa
    14101 ggcgaccgac tgcattcgcg taaattacga acctttaatg agccgccgtt ccgataataa
    14161 gttctgaacc ttcttatcag aaatagatgt gaaggagtaa gtaagacacc tggcaaatag
    14221 cctgcaataa tcgtgggctg tttgcttcct tgggcggata cgagttttat tatcgtctta
    14281 atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattata
//
LOCUS       DAPHDW010000115        14281 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.116, whole genome shotgun sequence.
ACCESSION   DAPHDW010000115 DAPHDW010000000
VERSION     DAPHDW010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14281
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.116"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            190..1761
                     /gene="garD"
                     /locus_tag="RH057_003375"
     CDS             190..1761
                     /gene="garD"
                     /locus_tag="RH057_003375"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HDV3129694.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPAPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLVGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMQCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMATRTELANRWFDLMDINAGTIATGEETIEEVGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            1910..2245
                     /gene="prlF"
                     /locus_tag="RH057_003376"
     CDS             1910..2245
                     /gene="prlF"
                     /locus_tag="RH057_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin PrlF"
                     /protein_id="HDV3129695.1"
                     /translation="MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHY
                     EILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNNPQKTRPFNIQQGKKLVAGMDVN
                     IDDEIGDDE"
     gene            2245..2709
                     /gene="yhaV"
                     /locus_tag="RH057_003377"
     CDS             2245..2709
                     /gene="yhaV"
                     /locus_tag="RH057_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ribonuclease
                     toxin YhaV"
                     /protein_id="HDV3129696.1"
                     /translation="MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKA
                     ATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKNKDWSRVKFGAGRYRFFFRYSEK
                     EKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH"
     gene            complement(2764..3573)
                     /gene="agaR"
                     /locus_tag="RH057_003378"
     CDS             complement(2764..3573)
                     /gene="agaR"
                     /locus_tag="RH057_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HDV3129697.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKHTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLNRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGE"
     gene            3822..5102
                     /gene="kbaZ"
                     /locus_tag="RH057_003379"
     CDS             3822..5102
                     /gene="kbaZ"
                     /locus_tag="RH057_003379"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HDV3129698.1"
                     /translation="MKHLTEMVRQHKAGKTNGIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCL
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLEDVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYYTACEGQ"
     gene            5125..5598
                     /gene="agaV"
                     /locus_tag="RH057_003380"
     CDS             5125..5598
                     /gene="agaV"
                     /locus_tag="RH057_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV3129699.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            5609..6388
                     /locus_tag="RH057_003381"
     CDS             5609..6388
                     /locus_tag="RH057_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HDV3129700.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGGTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAAAW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            6378..7256
                     /locus_tag="RH057_003382"
     CDS             6378..7256
                     /locus_tag="RH057_003382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019079236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HDV3129701.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDQSIGAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFILLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQTDVIDKLMPAFLPLVYTLTMFWLVRRGWSPLRLI
                     AVTVVLGIVGKFCHFL"
     gene            7274..7708
                     /locus_tag="RH057_003383"
     CDS             7274..7708
                     /locus_tag="RH057_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HDV3129702.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGEQSQFIAIDFPETSSTALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
     gene            7705..8838
                     /gene="nagA"
                     /locus_tag="RH057_003384"
     CDS             7705..8838
                     /gene="nagA"
                     /locus_tag="RH057_003384"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV3129703.1"
                     /translation="MTHVLRARRLLTEEGWLDDHQLRIADGVIAAIEPIPVGVTERDA
                     ELLCPAYIDTHVHGGAGVDVMDDAPDVLDKLAMHKAREGVGSWLPTTVTAPLNSIHAA
                     LKRIAQRCQRGGPGAQVLGSYLEGPYFTPQNKGAHPPELFRELEIAELDQLIAVSQHT
                     LRVVALAPEKEGALQAIRHLKQQNVRVMLGHSAATWQQTRAAFDAGADGLVHCYNGMT
                     GLHHREPGMVGAGLTDKRAWLELIADGHHVHPAAMSLCCCCAKERIVLITDAMQAAGM
                     PDGRYTLCGEEVQMHGGVVRTASGGLAGSTLSVDAAVRNMVELTGVTPAEAIHMASLH
                     PARMLGVDGVLGSLKPGKRASIVALDSGLHVQQIWIQSQLASF"
     gene            9189..10343
                     /locus_tag="RH057_003385"
     CDS             9189..10343
                     /locus_tag="RH057_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AgaS family sugar isomerase"
                     /protein_id="HDV3129704.1"
                     /translation="MPENYTPAAAATGTWTEEEIRHQPRAWIRSLTNIDALRSALNNF
                     LEPLLRKENLRIILTGAGTSAFIGDIIAPWLASHTGKNFSAVPTTDLVTNPMDYLNPA
                     HPLLLISFGRSGNSPESVAAVELANQFVPECYHLPITCNEAGALYQNAINSDNAFALL
                     MPAETHDRGFAMTSSITTMMASCLAVFAPETINSQTFRDVADRCQAILTSLGDFSEGV
                     FGYAPWKRIVYLGSGGLQGAARESALKVLELTAGKLAAFYDSPTGFRHGPKSLVDNET
                     LVVVFVSSHPYTRQYDLDLLAELRRDNQAMRVIAIAAESTDIVAAGPHIILPPSRHFI
                     DVEQAFCFLMYAQTFALMQSLHMGNTPDTPSASGTVNRVVQGVIIHPWQA"
     gene            10356..11216
                     /gene="kbaY"
                     /locus_tag="RH057_003386"
     CDS             10356..11216
                     /gene="kbaY"
                     /locus_tag="RH057_003386"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaY"
                     /protein_id="HDV3129705.1"
                     /translation="MSIISTKYLLQDAQANGYAVPAFNIHNAETIQAILEVCSEMRSP
                     VILAGTPGTFKHIALEEIYALCSAYSTTYNMPLALHLDHHESLDDIRRKVHAGVRSAM
                     IDGSHFPFAENVKLVKSVVDFCHSQDCSVEAELGRLGGVEDDMSVDAESAFLTDPQEA
                     KRFVELTGVDSLAVAIGTAHGLYSKTPKIDFQRLAEIREVVDVPLVLHGASDVPDEFV
                     RRTIELGVTKVNVATELKIAFAGAVKAWFAENPQGNDPRYYMRVGMDAMKEVVRNKIN
                     VCGSANRISA"
     gene            11383..11859
                     /gene="agaB"
                     /locus_tag="RH057_003387"
     CDS             11383..11859
                     /gene="agaB"
                     /locus_tag="RH057_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HDV3129706.1"
                     /translation="MSSPNILLTRIDNRLVHGQVGVTWTSTIGANLLVVVDDVVANDD
                     IQQKLMGITAETYGFGIRFFTIEKTINVIGKAAPHQKIFLICRTPQTVRKLVEGGIDL
                     KDINVGNMHFSEGKKQISSKVYVDDQDLADLRFIKQRGVNVFIQDVPGDQKEQIPD"
     gene            11898..12701
                     /gene="agaC"
                     /locus_tag="RH057_003388"
     CDS             11898..12701
                     /gene="agaC"
                     /locus_tag="RH057_003388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HDV3129707.1"
                     /translation="MHEITLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAIL
                     GDIQTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLP
                     FSLLMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCT
                     YLAQGAMQALVKAMPAWLTHGFEVAGGILPAVGFGLLLRVMFKAQYIPYLIAGFLFVC
                     YIQVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEEDYSNGI"
     gene            12691..13482
                     /gene="agaD"
                     /locus_tag="RH057_003389"
     CDS             12691..13482
                     /gene="agaD"
                     /locus_tag="RH057_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HDV3129708.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            13483..14238
                     /locus_tag="RH057_003390"
     CDS             13483..14238
                     /locus_tag="RH057_003390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HDV3129709.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT         3456 a         3615 c         3724 g         3486 t
ORIGIN      
        1 aggttgcgcg ctaaagcaca gatttgcgct ttaccttgcc gggcggcact gcaatccctg
       61 aaatgattga cattgatcac atttctgcgt ttaaactcct gacattctta tttcacccaa
      121 tgaagtcatt tatttttaaa tgagaccagg tcctcatttt aataacccct ggctggagaa
      181 tatagtacaa tggccaacat cgaaatcaga caagaaacgc caactgcgtt ttatataaaa
      241 gttcacgaca cagataatgt ggcaattatt gttaatgata atggcctgaa agcaggaacg
      301 cgttttccgg atggactgga attaattgaa catattcccc aggggcataa agtcgcattg
      361 ctggacattc cggctaatgg tgaaattatt cgttatggtg aagtgattgg ttacgccgtg
      421 cgtgcaatcc cacgcggaag ctggatcgac gaatcaatgg ttgtactacc ggaagcgccg
      481 ccgttacaca cgctgccact ggcaaccaaa gtcccggccc ccttaccgcc gctggaagga
      541 tacacctttg agggctatcg caatgccgat ggcagcgtgg gcaccaaaaa cctgctcggc
      601 atcaccacca gcgtccactg tgtggcaggc gtggtggact atgtagtaaa aatcattgaa
      661 cgcgatctgc taccgaaata cccgaacgtc gatggcgtgg tggggctgaa tcatttgtac
      721 ggctgtggcg tggcgattaa cgcaccggcg gcagttgtgc ctattcgtac catccacaat
      781 atttcgctga accctaactt tggcggcgaa gtaatggtga ttggtctggg ttgtgaaaag
      841 ttgcagcctg agcgcctgct ggtcggcacg gatgatgtgc aagctattcc agtagaaagc
      901 gccagcattg tcagtttgca ggatgaaaag catgtcggtt ttcagtccat ggtcgaggat
      961 attttgcagg tcgccgaacg ccatctacaa aaactgaatc aacgtcagcg agaaacctgt
     1021 ccggcttcag aactggttgt cggcatgcag tgcggtggca gcgatgcctt ttctggcgta
     1081 acggcaaacc cggcggttgg ctatgcgtct gatctactgg tgcgctgcgg cgcaacggtg
     1141 atgttctcag aagtcacgga agtgcgtgac gcgatccatc tgctgacacc acgcgcagtg
     1201 aacgaagagg tcggcaaacg gctgctggag gagatggagt ggtacgataa ctatctcaat
     1261 atgggaaaaa ccgaccgcag cgccaaccct tcgccgggca acaagaaagg cggtctggca
     1321 aacgtggtag agaaggcact cggctccatt gctaaatcgg gtaaaagcgc aattgttgaa
     1381 gtgctgtcgc ccggtcaacg cccgactaaa cgcggattaa tttacgccgc gacgccagcc
     1441 agcgattttg tctgtggcac gcaacaggtg gcttcgggta tcaccgtgca agtgtttacg
     1501 accggtcgtg gtacgccgta cggcctgatg gcggtacccg tcattaaaat ggcgacccgc
     1561 accgagctgg cgaaccgctg gtttgattta atggatatta acgcgggcac tatcgccacc
     1621 ggcgaagaaa ccattgaaga ggtgggctgg aagttgttcc actttattct cgacgtcgcc
     1681 agcgggaaga agaaaacctt ctcggatcaa tgggggctgc ataaccagct ggcagtgttt
     1741 aacccggcac cggtgacctg atttcttttg gaacgtccct cgcaaaacat ggccttagtg
     1801 ccatgttttt attgtttaaa gcccccacgt ccattaataa tgcatttgca ttaccttagt
     1861 tcaagcttat aatttgagca gaaaacaggc tgtaaaagga cagtgaatca tgcccgctaa
     1921 tgctcgctct cacgctgtac tgaccactga atcaaaggtc acgatacgcg gacaaacaac
     1981 tatccccgcg ccagtgcgtg aggccttaaa actgaagcca ggccaggaca gcattcatta
     2041 cgaaattctg cctggtgggc aagtctttat gtgccgactg ggagatgaac aggaggatca
     2101 tactatgaat gcatttttgc gttttctgga tgcagatatc cagaacaacc cgcaaaaaac
     2161 tcgtccattc aacattcaac aaggaaagaa acttgtcgct ggcatggacg tcaacattga
     2221 tgatgagatt ggcgacgacg aataatggat tttccacaaa gggttaatgg ttgggcgcta
     2281 tatgctcatc cctgttttca ggaaacctac gacgctttag ttgccgaagt cgagacatta
     2341 aagggaaaag atcctgaaaa ttatcagaga aaagccgcca caaagttatt ggcggtagtc
     2401 cataaagtga ttgaggagca tatcacggtc aatccatcat caccggcatt ccgtcatggc
     2461 aagtcgttag gctctgggaa aaataaagac tggtcacggg taaaatttgg tgctggtcgt
     2521 tatcgtttct tctttcgtta tagtgaaaaa gagaaagtca tcattctggg atggatgaac
     2581 gatgaaaaca ctctgcgcac ctacggtaaa aaaacagatg cctataccgt attcagcaaa
     2641 atgttaaaaa gaggacatcc tcctgccgac tgggaaagcc tcacccaaga gacagaagaa
     2701 aaccattgat ggtgttcaca atgcccgccg aacgtattcg tgttgaccag cgggcaccct
     2761 ttctcactcc ccgaccagaa tcacttcaac cccagccttt cgcagtcctt ccagactatc
     2821 cgcaggaatg ccttcatcaa caatgatcat gtcgatacgt tgagtatcaa tgatcttatg
     2881 taaactggaa cgattgaatt tactggaatc ggtgaccacg atgatccgtt ccgcaacttc
     2941 gcacatccga cggtttaaac gggcttcatc ttcattatgt gtgctgacgc cgcgctccag
     3001 atcgatcgca tctacaccaa gaaacagcat atcgaagtgg taattttgca gcgattgctc
     3061 agcctgatcg ccgtaaaaag attgcgactg acggcgcaaa tgcccgccgg tcatcagcag
     3121 ctcaacgcct tccgcttcca gcaacgcatt agccacgttc ataccgttgg tcatcgcaat
     3181 tacgtcagtg tgcttacgca tcagacgagc aatctcaaaa gtggtagtcc cggaatcgag
     3241 gatcacccga tgaccaggct gaatcaactc aacggcagct ttcgcaacgc tgcgtttcat
     3301 cgcggtgttc agcgcgcttt tatcttccac tgatggctcg actgacggcg tcgtgctatc
     3361 gcagatcaac gcgccaccat aggcacgcac agcgatcccc tgcttttcca gaaacgccag
     3421 atcattgcgg atcgtcacag tagatacgcc atacaatgcc gacagatcgt taacctgcac
     3481 actcccttgc tgtcgcagac gctgaatgat ctgttctcgt cgctcgctgg tgccagtcac
     3541 tcgcttctca cctgaagcgt cggtattact catagtaagt cctttcgtaa aactttcgtt
     3601 tcatttcgtt ttgcctatta acgcctttct attaagcaaa tgcaagccca ccttgcccat
     3661 tgacgcaagc tactctcgtt tcagtgactt tcattatgtt tcttttgtga atcagatcag
     3721 aaaactatta tctttcgttt tatttttatc tcaccatgac gcagtatcaa ctgaaacaaa
     3781 acgaaagatt aatatcgcag taatctgaac tggagaggaa agtgaaacat ctgacagaaa
     3841 tggtgagaca gcacaaagcg ggcaaaacaa atggaattta tgccgtttgt tccgcacatc
     3901 cgctggtgct ggaggctgca atccgctacg ccagtgcaaa ccaaacgccg ttactgattg
     3961 aagcaacctc caatcaggta gaccagttcg gcggttatac cggaatgacg cccgccgatt
     4021 ttcgcggctt tgtttgtcag ctcgccgact cgttgaattt cccgcaggat gcgttgattc
     4081 tgggtggtga ccatctgggg ccaaaccgct ggcaaaacct gccggccgct caggcaatgg
     4141 ccaatgccga tgatttgatt aaaagctacg ttgcggcagg attcaaaaaa atccaccttg
     4201 attgcagcat gtcctgtcag gacgatccga ttcccttaac tgatgacatc gtggctgaac
     4261 gcgccgcccg tctggcgaaa gtggcggaag aaacctgtct tgaacacttt ggcgaagccg
     4321 atctggagta tgtcattggt accgaagtgc cggtacctgg cggcgcgcat gaaaccttaa
     4381 gcgagctggc ggtcaccacg ccggatgccg cccgcgccac gctggaagcc catcgtcacg
     4441 cctttgaaaa gcaaggtttg aatgccatct ggccacgcat cattgccctg gtggttcaac
     4501 ccggcgtcga attcgatcac accaacgtta ttgattatca gcccgccaaa gcgagcgcct
     4561 taagccagat ggtcgaaaac tacgaaacgc tgattttcga agcgcactct accgattatc
     4621 aaacgccgca atcgctgcgc cagctggtga ttgaccactt tgccattctg aaagttggcc
     4681 cagcgctgac cttcgccctg cgtgaagctc tgttctctct ggcggcgatt gaagaagaac
     4741 tggtgccagc gaaagcctgt tctggtctgc gtcaggtgct ggaagacgtg atgctcgacc
     4801 gcccggaata ctggcaaagc cactaccacg gtgacggcaa cgcgcgtcgt ctggcgcgtg
     4861 gttatagcta ctcggatcgc gtgcgctatt actggccgga cagccagatt gatgacgctt
     4921 tcgctcatct ggttcgtaat ctggcggatt caccaattcc gctaccgctc atcagccagt
     4981 atctgccgct gcaatacgtg aaagttcgct ccggcgagct gcaaccgacg ccacgggaac
     5041 tcattatcaa ccatattcag gacatcctgg cgcagtacta cacagcctgt gaaggccaat
     5101 aagcaaaaca aagaggaaca cgctatgcca aatattgttt taagccggat tgatgaacgc
     5161 ttgattcacg gtcaggtcgg cgttcagtgg gtcggatttg cgggggcaaa tctggtgctg
     5221 gtagccaacg atgaggttgc cgaagatccg gtacaacaaa acctgatgga aatggtactg
     5281 gcagaaggga tcgccgtacg tttctggacg ctgcaaaaag ttatcgacaa cattcatcgc
     5341 gccgccgatc gacagaaaat cctgctggtt tgtaaaacac ccgccgattt cctgacgctg
     5401 gtgaaaggtg gcgttccggt gaatcgcatt aacgttggca atatgcacta cgcaaatggc
     5461 aaacaacaaa tcgccaaaac ggtttctgtg gatgcgggcg atatcgcagc atttaacgac
     5521 ctgaaagccg ctggggtgga atgcttcgtt cagggcgtcc cgacagagcc tgctgtggac
     5581 ctctttaaat tactttgagg gattcatcat ggaaatcagc ctgttgcagg catttgcgtt
     5641 gggcattatc gcctttatcg ctggcctgga tatgtttaac ggcctaacac atatgcaccg
     5701 cccggtggtc ctcggcccgt tggtcgggct ggtacttggc gatctgcata ccggaatttt
     5761 aaccggcggt acgctggaac tggtgtggat ggggctggcc ccgctggcgg gcgcacagcc
     5821 gcctaacgtg attatcggta ctatcgtcgg cacggcgttt gccattacta ctggcgtgaa
     5881 acccgatgtc gcggtcggtg tcgccgtacc tttcgctgtc gcagtacaga tggggattac
     5941 cttcctgttc tcggtgatgt ctggcgtgat gtctcgctgc gaccggatgg cggagaacgc
     6001 cgatactcgc ggcattgaac gcgtgaacta tctggcactg ctggcactcg gcactttcta
     6061 ttttctctgc gctttcctgc ctatctactt cggcgcggaa catgcaaaaa ccatcattga
     6121 tgtcctgccg caacgattaa tcgacggcct cggcgtcgcg ggcggcatca tgccagcaat
     6181 cggttttgcc gtgctgctga aaatcatgat gaaaaacgtc tacatcccct acttcatcct
     6241 gggctttgtt gccgcagcct ggctcaagtt accggtgctg gctattgctg ccgctgcgct
     6301 ggcgatggcg ctgatcgacc tgctgcgtaa atctcctgaa ccgactcaac ctgcggcaca
     6361 gaaagaggaa tttgaagatg gcatctaatc aaaccaccct gccgaacgtc agtgaaaacg
     6421 aagaaacatt gctgactggc gtcaatgaaa acgtgtatga agatcagagc attggcgcgg
     6481 agctgacgaa aaaagatatc aaccgcgtcg cctggcgttc catgctgtta caagcgtctt
     6541 ttaactacga acgtatgcag gcttctggct ggttgtacgg tctgctgcct gctctgaaaa
     6601 agatccacac taataaacgc gacctggcgc gcgccatgaa ggggcatatg ggtttcttca
     6661 atacccatcc gtttctggtg acatttgtta tcggcattat ccttgcgatg gagcgttcta
     6721 agcaggacgt taacagtatt cagagcacca aaattgccgt cggtgcgccg ctcggcggga
     6781 ttggcgatgc aatgttctgg ctaacgctac tgccgatttg tggcgggatt ggagccagtc
     6841 tggcattgca aggctccatt cttggcgcag tcgtctttat tctgctgttc aacgtggtac
     6901 acctggggct gcgttttggt ctggcgcatt atgcttaccg catgggcgtg gcggcgattc
     6961 cactcattaa agcgaatacc aaaaaagtcg gccatgctgc gtctatcgtt gggatgacgg
     7021 taatcggcgc gctggtggca acctatgttc gtttaagcac cacgctggaa atcacagcgg
     7081 gcgacgcagt ggttaagtta cagactgatg ttattgacaa actgatgcct gccttcttac
     7141 cgctggtcta caccttgacc atgttctggc tggtacgccg cggctggagt ccgctgcgtc
     7201 tgattgcagt taccgtggtt ctcggcatcg tcggtaaatt ctgccatttc ctttaaatac
     7261 aatgaggtat tagatgttaa gtattatttt gacagggcat ggcgggtttg ccagcggcat
     7321 ggaaaaagca atgaagcaaa ttctcggtga gcaatcgcag tttatcgcca tcgattttcc
     7381 ggaaacctca agcaccgcgc tgcttacatc gcagcttgaa gaggccatcg cccaactgga
     7441 ttgtgaagac ggcattgttt ttctgacgga tttgctgggc ggcacgccgt ttcgcgtagc
     7501 ttcaacgctg gcgatgcaaa aaccgggctg tgaagtgatc accggcacca atttgcaact
     7561 gctactggag atggtgctgg agcgcgaagg gttaagcggt gaagaattcc gtgtacaggc
     7621 gctggagtgt ggacaccgag ggctgaccag tctggtggac gagttaggcc gctgccatga
     7681 agaatgtccg gtcgaggaag gaatatgaca cacgttctgc gcgccagaag gctgctgact
     7741 gaagagggat ggctcgatga ccatcagttg cgtattgctg acggtgtcat cgcagcaatc
     7801 gaaccgattc cagtgggcgt gactgaacgc gatgcggaac tgctctgccc cgcttacatc
     7861 gacacccatg tacacggtgg tgcgggcgtt gatgtaatgg atgacgcgcc ggatgtactc
     7921 gacaagctgg caatgcacaa ggcacgcgaa ggtgtcggca gttggttgcc gactaccgta
     7981 accgcgccgc ttaacagcat tcatgcggcg ctgaaacgta ttgctcaacg ttgccaacgc
     8041 ggcggacctg gtgcgcaagt gctggggagt tatctcgaag gaccgtactt cacgccgcag
     8101 aataaaggcg cgcatccgcc ggaattgttt cgcgagcttg aaattgccga gctggatcaa
     8161 ttgattgccg tttctcagca caccttacgc gtggtagcgc tggcaccgga aaaagagggg
     8221 gcattgcagg ccatccgcca tcttaaacag caaaatgtac gagtgatgct ggggcatagc
     8281 gcggcgacct ggcaacaaac tcgcgccgcg tttgatgctg gtgccgacgg cctggtgcat
     8341 tgctataacg ggatgacagg tttacatcac cgcgaaccgg gaatggttgg cgcgggatta
     8401 acggacaagc gcgcctggct ggaactgata gccgatggtc atcatgtgca tccggcggcg
     8461 atgtcgctgt gttgttgctg tgcaaaagag agaatcgtgc tgatcaccga cgcgatgcag
     8521 gcagccggga tgccggatgg tcgctatacg ttatgtggcg aagaagtgca gatgcacggt
     8581 ggcgttgtcc gtaccgcgtc cggtgggctg gcgggcagta cgctgtctgt tgatgcggca
     8641 gtgcgcaaca tggtcgagtt gacgggcgta acgcctgcgg aagccattca tatggcatcg
     8701 ctgcatccgg cgcgaatgct gggtgttgat ggtgttctgg gatcgcttaa accgggcaaa
     8761 cgcgccagca tcgttgcgct ggatagcggg ctgcatgtgc aacaaatctg gattcagagt
     8821 caattagctt cgttttgata ttttgctcct ttattgggcc ttcacttccc ccgtaaggcc
     8881 tttctttttc tttcgttttg atctgtgcag aggtgtcgga tgcgacgcta acgcgtctta
     8941 tgcgacctac ggtacgccgc atccagcaat cggtgaacat agcgacgtca ttacgcaatt
     9001 ctttaatctt cccttgtctt ttctttcaat gatcacagat ttcgttttat ttcctttttc
     9061 tccattgaac tttcggtttc ttttctatag attttaatca acgaaagaca tcaccaagtg
     9121 aaatgaaacg aaaggcaagt gaaagcgaca acgcccgacg tcaagttcat cagactaagg
     9181 attgagttat gccagaaaat tacacccctg ctgccgccgc aaccggtaca tggactgaag
     9241 aagagatccg ccatcagcct cgcgcatgga tccgttcact caccaacatc gacgcgctac
     9301 gttctgcgct caataacttc cttgaaccgt tactgcgcaa agagaatctg cggatcatcc
     9361 tgaccggagc cggaacctcg gcatttatcg gtgacatcat cgcgccgtgg ctcgccagcc
     9421 ataccggtaa aaacttcagc gccgtaccga ccaccgatct ggtcaccaat ccgatggact
     9481 acctgaaccc agctcatccg ctgctgttga tctccttcgg tcgatccggc aacagcccgg
     9541 aaagcgtcgc tgccgtggaa ctggcaaatc aatttgtacc agaatgctat cacctgccga
     9601 tcacctgcaa cgaagcgggc gctctttacc aaaacgcgat caacagcgat aacgcgtttg
     9661 ccctgctgat gcccgcagaa acgcacgatc gcggctttgc gatgaccagc agcattacca
     9721 ccatgatggc cagctgcctc gcggttttcg cacctgagac gatcaacagc caaaccttcc
     9781 gcgacgtggc ggatcgttgc caggcgatcc tgacctcact gggcgatttc agcgaaggtg
     9841 tgtttggtta cgcaccgtgg aaacggatcg tttatctcgg cagcggtggc ttacagggcg
     9901 cagcacgcga gtcggcgctg aaagtgctgg aactgactgc gggtaaactg gcggccttct
     9961 atgattcccc gaccggattc cgtcatggcc cgaaatcact ggtcgataac gaaacgctgg
    10021 tggtggtatt tgtctcaagc cacccttaca cccgtcagta tgatcttgat ctgctggctg
    10081 aactccgccg tgacaaccag gcaatgcgcg taatcgccat cgccgcggaa agcaccgaca
    10141 tcgtcgctgc cggtccacat attatcctgc cgccgtcacg tcactttatc gacgttgagc
    10201 aggcattttg cttcctgatg tacgcccaga cgtttgcact gatgcagtcg ctgcacatgg
    10261 gcaatacgcc ggatacccca tcagccagcg gcaccgttaa ccgcgtggtg caaggcgtaa
    10321 tcattcatcc gtggcaggca taagaggatc gcattatgag cattatctcc actaaatatc
    10381 tgttacagga cgcccaggcc aatggctacg cggtgcctgc ttttaacatt cataacgccg
    10441 agacgatcca ggcgatcctc gaagtgtgca gtgaaatgcg atcgccggtg atcctcgccg
    10501 gaacgccggg gacctttaaa catatcgcgc tggaagagat ctacgccctg tgcagcgcct
    10561 attccacaac ctacaacatg ccactggcgc tgcatctcga ccaccacgaa tcgctggatg
    10621 atattcgccg taaagtccac gcaggtgtgc gcagcgcaat gatcgacggc agccacttcc
    10681 cgtttgccga gaacgtgaag ctggtgaaat cggttgttga cttctgccac tcacaggatt
    10741 gcagcgtgga agcagaactg ggccgcctgg gcggtgttga agatgacatg agcgttgacg
    10801 ccgaaagtgc attcctgacc gatccacaag aagctaaacg ctttgtcgaa ctgactggcg
    10861 tcgacagcct ggcggtagcg attggtacgg cgcacggctt atacagcaaa acgccgaaga
    10921 ttgatttcca gcggctggcg gaaattcgtg aagtggtgga tgttcctctg gtgctacatg
    10981 gtgccagcga tgttccggat gaatttgtcc gtcgcactat tgaacttggc gtcacaaaag
    11041 tgaacgttgc cacagaatta aaaatagcct tcgctggcgc ggttaaagcc tggtttgcgg
    11101 aaaatccgca gggtaatgat cctcgttatt atatgcgcgt cggaatggat gcgatgaaag
    11161 aagttgtcag aaataaaatt aatgtctgtg gttcagcgaa tcgaatttca gcataatcat
    11221 tagtttttta tgatttatcc caatgtactt cccgattaat atcggggagt gccttaatgg
    11281 aaaaggagat gactaaacct taataaatac atcagtacaa tatcgcaaca ataatatatt
    11341 aaaaaaaatt atattattca actttatggt gaggattaca caatgagcag tccaaatatt
    11401 ctcttaaccc gtattgataa ccgtctggtt cacggtcagg ttggcgtgac ctggacatcc
    11461 accatcggtg cgaatctgct ggtagtcgtg gatgatgttg tcgctaacga tgatattcaa
    11521 cagaaattaa tgggtattac cgcagaaacc tacggctttg gcattcgttt ctttactatc
    11581 gaaaaaacca ttaacgtcat cggcaaagct gcaccgcatc agaagatctt cctgatttgc
    11641 cgtacgccac aaacggtacg taaattggta gaaggtggca ttgacctgaa agatatcaac
    11701 gtcggcaata tgcatttctc ggaagggaaa aagcaaatca gcagtaaagt ttatgtcgat
    11761 gaccaggatc tcgcggactt acgttttatt aaacaacgtg gcgtgaatgt tttcattcag
    11821 gacgtccctg gcgatcaaaa agaacaaatc cctgactaaa tctaaaatcg ccttaatatt
    11881 ggtttgaggt aataaaaatg catgaaataa ccctacttca gggattatcc ctggcggcgt
    11941 tagttttcgt tctggggatt gatttttggc tggaagcctt atttttattc cgcccgataa
    12001 tcgtttgtac cctaactggc gctattctcg gtgatattca gactggctta attaccggtg
    12061 gtctgacaga gttggctttc gccggattaa cccctgcagg tggtgttcag ccgcccaacc
    12121 cgattatggc gggtctgatg accaccgtca ttgcatggtc tacgggcgtt gatgccaaaa
    12181 cagcaattgg tcttggcctg ccgtttagtt tgttaatgca gtacgtcatt ctgttcttct
    12241 attccgcttt ctcattattt atgaccaaag ccgataaatg cgcgaaagag gcggatacgg
    12301 cagcgttttc ccggcttaac tggacaacga tgctcatcgt cgcttcagcg tatgcggtga
    12361 ttgctttcct ctgtacttac ctggcacaag gggcgatgca ggcgctggtg aaagcgatgc
    12421 ccgcctggtt gacccacggc tttgaagtgg ctggcggtat tctgcctgcc gttggttttg
    12481 gcttgctgct gcgcgtaatg ttcaaagcgc aatatatccc ttacctgatc gccggtttcc
    12541 tgtttgtttg ctacatccag gtcagcaacc tgttgccggt tgccgtactg ggcgcaggct
    12601 ttgcggtgta tgagtttttc aatgcgaaat cccggcagca agcgcaaccg cagcccgttg
    12661 ccagtaaaaa tgaagaagag gactacagca atgggatctg aaatcagtaa aaaagatatc
    12721 acccgtctgg gctttcgttc gtcgctgctg caagcgagct ttaactacga aaggatgcag
    12781 gcgggcggtt ttacctgggc gatgttgccg atcctgaaaa agatttataa ggacgacaaa
    12841 ccgggcttaa gcgcggcgat gaaagataac ctcgaattta ttaataccca cccgaatctg
    12901 gtcggattcc tgatggggtt attaatttcg atggaagaaa aaggagaaaa ccgcgacacc
    12961 attaaaggcc tcaaagtggc actgtttggc ccaatcgccg ggattggcga tgcgattttc
    13021 tggtttactt tgttgccgat tatggcggga atttgctcat catttgccag ccagggaaac
    13081 ctgctggggc cgattctatt tttcgccgtt tacctgctta tctttttcct gcgcgtcggc
    13141 tggacccacg tcggttattc agtcggcgtg aaggcgatcg ataaagtgcg agagaactcg
    13201 cagatgattg ctcgctcggc aaccatcctc gggatcacgg taatcggcgg gctgatcgct
    13261 tcgtatgtgc atattaacgt ggtgacatcg tttgccatcg acagtaccca cagcgtcgcg
    13321 ctgcaacagg atttcttcga taaagtcttc ccgaacattt taccgatggc ctacaccctg
    13381 ctgatgtatt acttcctgcg ggtgaaaaaa gcgcatccgg tgctgttaat cggcgtgact
    13441 tttgtgctct ctattgtttg ttccgcattc ggcattttgt aaatggaacg aggcactgct
    13501 tctggcggtg cctctttact taaggaattt catcctgtgc aaacccttca gcaagttgaa
    13561 aactatacgg cgttaagtga acgagccagc gaatatttat tggctgtgat ccgtagcaaa
    13621 ccggatgccg tgatttgcct ggcgaccgga gccacgccat tactgacgta tcattatctg
    13681 gtagaaaaaa tccaccagca gcaggttgat atcagccagc tcaccttcgt gaagctcgac
    13741 gaatgggtgg atctgccatt aacgatgcct ggcacctgcg aaactttcct acaacagcat
    13801 atcgtgcagc cgctggggct gcgtgaagac cagctcatca gctttcgctc cgaagagata
    13861 aatgagacag agtgcgaacg ggtaacgaac ctgattgcgc gcaaaggcgg tctggattta
    13921 tgcgttctag gattggggaa aaacggtcat cttgggctga acgaaccggg agaaagccta
    13981 caaccggcct gccatatcag tcaacttgat gccagaacac agcaacatga gatgttaaaa
    14041 accacgggtc gccccgtgac tcgtgggatc accttaggcc tgaaggatat tctcaatgcc
    14101 cgcgaggttt tgttattggt gactggcgaa ggaaagcagg atgcgacaga acgttttctc
    14161 acggctaaag tctccaccgc tatcccggct tcgtttttat ggctgcacag taactttatt
    14221 tgtttaatta atacctaacc agatgaaaat ttgctcaaaa tttttaaatt aacttattca
    14281 a
//
LOCUS       DAPHDW010000116        14210 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.117, whole genome shotgun sequence.
ACCESSION   DAPHDW010000116 DAPHDW010000000
VERSION     DAPHDW010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14210
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.117"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            265..1410
                     /locus_tag="RH057_003391"
     CDS             265..1410
                     /locus_tag="RH057_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV3129710.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1504..2037
                     /locus_tag="RH057_003392"
     CDS             1504..2037
                     /locus_tag="RH057_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV3129711.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(2034..2351)
                     /gene="sugE"
                     /locus_tag="RH057_003393"
     CDS             complement(2034..2351)
                     /gene="sugE"
                     /locus_tag="RH057_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV3129712.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            3079..8565
                     /locus_tag="RH057_003394"
     CDS             3079..8565
                     /locus_tag="RH057_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDV3129713.1"
                     /translation="MINFKPKIPAMLGALAVLTAGAAHAELLEYTFKAPDGTQRSLTP
                     NANYANPTGNISFALSAGIDRKVKISVLRSDGTVVSTATSHLLGATDRITVGGKSYYG
                     AELQLPAPVGGAYTIRAEILASDGSTVQTDEYPLTVDVTPPTYSSLAPVYSNYGQVTS
                     GDVWKLGLGGSEDNAFLLSGISDESPIKGVKAKLYRQDGSLYKDVSVNYDDANGQARQ
                     SFQSGFFPASDLDEVFTLQFEISDSAGNSYLSPRQKVMFDSITNAPSAPFGVYDPSST
                     NNLGPGLTGFVAYTEGMTVKTNPIKLAWRVPRDNWHEYREGGINMTNALGEMSKVGED
                     ASYVYLVTTAPYGNTDGNYWRWVNFGQWGGGGIAYNLTLSPSAPKSPRLLGVDYNYSD
                     IGWSSFYRYWVNNSVLPVTVSSIRVKVEPRPYVQTAVHRGSCEIPVGQDSCVIANSFT
                     MAKGTTGYVHDNATVFNPDKSLRSNPLWAEVNWNDQHYPQLSQQFDQNSKVFTLFVNQ
                     PGRGAYFDRLRLRSAWIEDSKGNKLSPTGGLIANNWENYTYQWDLKTLPEGQYSLVAA
                     AEEMHGPLTRQPMFQITSDRTPPTMTLSVADGAAIQTLDDVVITLADAIDPSPKLTSI
                     ALVGGPANDKVQLSWREESKGRFRLEYPVMFPSLKEGESYTLTVSGEDAQGNAVQKAV
                     GFEYKPRQVMLADGMDGKVMVPAVTHEFVHADGKRIIETKPLTLSDGAVVTGSYDVFA
                     TLRSDAKVPLVVNGVRIEPGQTMGIMSQHDFGASGGRLSIPVKPAVPDVVGSSSLLVM
                     TSAPNSPILVVDINTWKGTAKLSAESWTIRQVIDPVKIYALPESGVPCRFTTKEDVAM
                     AADPIRDPVCLLQWDRTPDEAEQTTQDNNGMKVAGLVGQAVSIGEQPVEYSLYLFSGD
                     GSKVKVGSGSQNLTVTTAYGSVGYTPIDDIAQVNRVIEDFDVNFKQSKGPDCSITLSA
                     DRAKKEAANKAVGSASRACLFEWQQIPDGLVQDPLSESPSLSGSLASNGVHPLGWRVS
                     IFTRNGTRVTLNDETFNVEAVDPPAPTVELASDYNFKDNIYLVPMTGNYLGDAIINSE
                     RADLDIAISRNSDVLESETFTPGWGATNKVYRRINTDERALWEETTYKVNAAYNKVPD
                     VKTEVVYRAISAPSDSIRPIVEVKGDTAIDTQALPVRVLIRDQYKPDGDYDANTMGVW
                     KVRLIQQKAYNETVALTDYAEASNGEAQFSVDLSGVDTSSVRIAAEAVLESPVEGYNR
                     TELSIRPAFLTVLRGGAIGAGVEARKLSGEAPFTAVFKLSLDDRQDLRATGQVVWETS
                     KDDGKTWEQFIPEDRYKYQLVKTFDKGEYQVRAKVVNVNSGAEKYTEAVSVVAYDKPD
                     IAVIGPTTLFVGSEGKYTANLTLNDEPISGGNAIVEWSTDGGKTYAQTGDSITLSSDE
                     ETRYRLWARVRSATAPADDGYAYEVAKTAVDFRAVKAPRPYVTGPRVIETGKKYVFKA
                     ETSLPYRGMDVKLNGFFTLPDGSIVQGDTAEYEPSDTDLNQATVETKYTTWIEGYRDQ
                     GAEASHSLRSRVWQYVWPSFGMQVRKNADVAPATITASVRPIAFNGKLEEPTYEWELP
                     EGAVIQDQRQDIVRSFVINEPGDYNIKVTVRDARGHETVIEQPLKIGQAAPYAIDLQY
                     SGSNKYEREPLDVLLRPYISGGHPRDRISTRVYSVDGTPLESSGYYGRATLGAGEHSI
                     KLKITSEMGHEAEGEVNINVAENKLPACSLSSRETVGSWIVYANCEDTDGRMKSYEWT
                     IAGELQSISSDRVTISKGTYETMPTISLVGVDDSGGKSEAVTMN"
     gene            8714..9421
                     /locus_tag="RH057_003395"
     CDS             8714..9421
                     /locus_tag="RH057_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HDV3129714.1"
                     /translation="MRTKLLGALMVFGIITGTAHASSKLEITDPRAAKIEDIVELPIK
                     GVRAVQSDGQIMFLSENGRFVISGQIYDLWSKKPLNTMSQMRDVAERIHFKSMGMDVD
                     TLNTVSMGRGDKEVVVFVDPRCAVCHQLMGDAKSLVDDYTFKFIVIPALGAESNRLAK
                     NLYCAKDKTHALDALMNNTLGSLPSKETCDPGQYDQTLLTAHFIGIEGVPFVVAPDGR
                     VSKGRPKNLKSWLESAE"
     gene            9418..10119
                     /locus_tag="RH057_003396"
                     /pseudo
     CDS             9418..10119
                     /locus_tag="RH057_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
     gene            10420..10764
                     /locus_tag="RH057_003397"
     CDS             10420..10764
                     /locus_tag="RH057_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HDV3129715.1"
                     /translation="MTKRQRKNRKRINRLVEHWPELFNREKPQPRKVEIPDDLIQDIA
                     IRELAFSAGALRAAVASYVQSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLM
                     VLNDRQKARKPR"
     gene            complement(10783..11331)
                     /locus_tag="RH057_003398"
     CDS             complement(10783..11331)
                     /locus_tag="RH057_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129716.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTACPSSPVVLRWLREQHGGATIRCLLLLSQTSICH
                     PRASATVDEECSQRVDLLQQTWKVISAGGQCQVERCFRVARGDTSGQYVALKTVALSL
                     GLPVVTAITHRPVQRCTLITAQ"
     gene            complement(11600..11836)
                     /locus_tag="RH057_003399"
     CDS             complement(11600..11836)
                     /locus_tag="RH057_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3129717.1"
                     /translation="MLTSITGHDSVLLRAEDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(11905..12477)
                     /locus_tag="RH057_003400"
     CDS             complement(11905..12477)
                     /locus_tag="RH057_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129718.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKPYQKQITRDVVLDRRSPDCEALFKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPWYPVAGQL"
     gene            complement(12735..13301)
                     /locus_tag="RH057_003401"
     CDS             complement(12735..13301)
                     /locus_tag="RH057_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDV3129719.1"
                     /translation="MPKSYTPNWFFTALLDNHINQLVARYSRLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWLDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRKPHF"
BASE COUNT         3723 a         3462 c         3660 g         3365 t
ORIGIN      
        1 gtatcattgc agcaaagatg aaatcaatga tttatcaaaa atgattgaaa ggtggttgta
       61 aataatgtta caatgtgtga gaagcagtct aaattcttcg tgaaatagtg atttttgaag
      121 ctaataaaaa acacacgtgg aatttaggaa aaacttatat ctgctgctaa atttaaccgt
      181 ttgtcaacac ggtgcaaatc aaacacactg attgcgtctg acgggcccgg acaccttttt
      241 gcttttaatt acggaactga tttcatgatg aaaaaatcgt tatgctgcgc tctgctgctg
      301 acagcctctt tctccacatt tgctgccgca aaaacagaac aacagattgc cgatatcgtt
      361 aatcgcacca tcaccccgtt gatgcaggag caggctattc cgggtatggc cgttgccgtt
      421 atctaccagg gaaaacccta ttatttcacc tggggtaaag ccgatatcgc caataaccac
      481 ccagtcacgc agcaaacgct gtttgagcta ggatcggtta gtaagacgtt taacggcgtg
      541 ttgggcggcg atgctatcgc ccgcggcgaa attaagctca gcgatccggt cacgaaatac
      601 tggccagaac tgacaggcaa acagtggcag ggtatccgcc tgctgcactt agccacctat
      661 acggcaggcg gcctaccgct gcagatcccc gatgacgtta gggataaagc cgcattactg
      721 catttttatc aaaactggca gccgcaatgg actccgggcg ctaagcgact ttacgctaac
      781 tccagcattg gtctgtttgg cgcgctggcg gtgaaaccct caggaatgag ttacgaagag
      841 gcaatgacca gacgcgtcct gcaaccatta aaactggcgc atacctggat tacggttccg
      901 cagaacgaac aaaaagatta tgcctggggc tatcgcgaag ggaagcccgt acacgtttct
      961 ccgggacaac ttgacgccga agcctatggc gtgaaatcca gcgttattga tatggcccgc
     1021 tgggttcagg ccaacatgga tgccagccac gttcaggaga aaacgctcca gcagggcatt
     1081 gcgcttgcgc agtctcgcta ctggcgtatt ggcgatatgt accagggatt aggctgggag
     1141 atgctgaact ggccgctgaa agctgattcg atcatcaacg gcagcgacag caaagtggca
     1201 ttggcagcgc ttcccgccgt tgaggtaaac ccgcccgccc ccgcagtgaa agcctcatgg
     1261 gtgcataaaa cgggctccac tggtggattt ggcagctacg tagccttcgt tccagaaaaa
     1321 aaccttggca tcgtgatgct ggcaaacaaa agctatccta accctgtccg tgtcgaggcg
     1381 gcctggcgca ttcttgaaaa gctgcaataa ctgacgatga ggcccaggat attgggcctc
     1441 ctttctttct ctttttttcc tgttgtcatc tacacttaac aaaaatacag caaggaaaat
     1501 cccatgcgca ttttgcccgt cgttgctgca gttacggctg cattcctggt tgtcgcgtgt
     1561 agctccccga caccgccgaa aggcgttacc gtggtaaata actttgatgc caaacgctat
     1621 ctgggaacct ggtatgaaat tgcgcgcttc gaccatcgtt tcgagcgcgg attggataaa
     1681 gtgaccgcaa catacagctt gcgcgacgac ggcggcatca acgttattaa caagggctat
     1741 aaccctgaca gggagatgtg gcagaaaacg gaagggaaag cctatttcac cggcgaccca
     1801 agcagagccg cgcttaaggt ttcttttttc ggccccttct atggcgggta taacgtaatt
     1861 gcactcgacc gggaatatcg tcacgcgctg gtttgtggtc cggatcgcga ctacctgtgg
     1921 atcctttcac ggacccctac tatttcagat gaaatgaaac agcaaatgtt agccatcgcg
     1981 acccgggaag ggtttgaagt gaataaactg atttgggtga aacagcctgg cgcttagtga
     2041 gtgctcagct tcagaccaat aatgccagca acgatcagcc caaggctcag caaacgtgcc
     2101 gggctggcag actcacccag cagcaaaatc cctgtaatgg ccgccccaac agcgccaata
     2161 ccggtccaga ccgcataagc ggttcctaca ggcaacgtgc gcattgccca agagagcatg
     2221 gcgatactga cgatcatcgc cgcaatagtg ataatgcttg gcgtaagacg cgtaaaaccg
     2281 tgggtgtatt tcaggccaat cgcccagaca acttcgagca aacctgcaat taataaaacg
     2341 atccaggaca tatcaggctc cagaacaatg gggccgtccc cggtgaaaga agcgtttgca
     2401 ggtcgtcctg caaagctaat gtgtgaaatg gcatttttgc ccggaagaaa atgaatttca
     2461 acctttttat tcaccgcctg ctaaaagcaa gaattaagca taattagcgg cgtagttccc
     2521 gcatttggca ctcatcggag ttctctatga tgaagggctt tctgaaacca gaaagtcgat
     2581 tgcccctctt ctgaatgcga aacgtttatg cttaaaattt tagtgattga ccggtgccac
     2641 tttacccgca cagggataga ggccttgctt aatcattctg gcaggttcag ctcctcattt
     2701 ctggtatcag gaatcaataa tctcctgtta gcaaaagagc atattttgca atggaaaccg
     2761 catctggtga tcgcggattt atacagtttt attagcgaga cgcactccag tccgccaatt
     2821 aaaccttttt ttatgagttg tggcgtcatc ccactaattt tactgcaatc agcagacaga
     2881 cagcatgcac ccatcgtgcc ctctcaatcc gtagcccatt cggtcttgac caagcacacc
     2941 acgctgaaca cactctccca cacaattcag gatttcctta tttgctcaaa aagggcactt
     3001 ccacagccca tgtttatgca ctccctcccg taacctccag cattgggttc ttgtaactca
     3061 aaacgaatgg agaatgcgat gatcaatttc aagcctaaaa ttccggcgat gcttggggct
     3121 ctggcggttc ttaccgctgg tgctgctcat gcagagctgc tggaatacac ctttaaggct
     3181 ccagatggta cgcaacggtc tctgactccg aatgccaact atgccaaccc aacgggcaat
     3241 atctcgtttg ccttgagtgc cggtatcgac cgaaaggtaa agatttcggt gcttcggtcg
     3301 gatggaacag tggtttcgac agcgaccagc caccttcttg gggctactga tcgcatcaca
     3361 gtgggtggaa aatcctacta tggtgcggaa cttcaactac cagctccagt tggtggggca
     3421 tacactatca gggcggaaat tcttgcctct gatggttcta cagtacagac ggatgaatac
     3481 cctcttactg tcgatgtgac gccgccaacc tactcttcgc ttgccccggt ttatagtaac
     3541 tatgggcagg ttactagcgg tgatgtttgg aaactgggtc taggtggctc tgaggataat
     3601 gcctttttgc tgtcaggaat ctctgatgag tcacccatca aaggcgtcaa agcaaagctg
     3661 tatcgtcaag acggttcact ctacaaagat gtatccgtga actacgatga cgctaacgga
     3721 caagctagac agtctttcca atctggcttt ttcccagcct ctgacttgga tgaagtgttt
     3781 acacttcagt ttgagataag cgatagcgct ggaaatagct atctttcacc tcgtcaaaaa
     3841 gtgatgtttg atagcataac caatgctccg tctgcacctt ttggtgttta tgacccttca
     3901 agtacaaaca acttaggccc tggattaacc ggatttgttg cttacactga gggcatgaca
     3961 gttaaaacta acccaatcaa gttagcttgg cgtgttccgc gtgataactg gcatgagtat
     4021 agagaaggtg ggataaatat gaccaatgcc ttgggagaga tgtccaaggt aggagaagat
     4081 gccagctatg tttatctcgt cacaaccgca ccttatggca ataccgacgg caattattgg
     4141 cgttgggtga acttcggcca gtggggggga ggcggtattg cttataacct cacactctct
     4201 ccatcagcgc ctaaaagtcc cagacttttg ggtgtggact acaattacag tgatatagga
     4261 tggtctagtt tttaccgcta ctgggtgaac aattctgttc ttcctgtcac tgttagtagt
     4321 atccgtgtca aagttgaacc aaggccttac gttcaaactg ccgttcacag gggaagctgt
     4381 gaaattcctg ttggtcaaga tagctgcgtg attgcaaata gcttcacaat ggctaaaggc
     4441 acaacagggt acgtccacga taatgcgaca gttttcaatc cggataaatc tcttcgctcg
     4501 aatcctcttt gggcagaggt taactggaac gatcagcact accctcagct aagtcagcag
     4561 ttcgatcaaa atagcaaggt atttacctta ttcgttaacc agccaggacg aggcgcttac
     4621 tttgataggc tgcgtctgcg aagtgcatgg attgaagata gcaaaggcaa caagctgtct
     4681 ccaactggtg ggttaatagc caacaactgg gagaactaca cttaccagtg ggatcttaaa
     4741 actctccctg aggggcaata cagtcttgtt gccgcagcag aggagatgca cgggccttta
     4801 acgcgacaac caatgttcca gataacgtct gatagaacgc caccaaccat gacactcagt
     4861 gtggcagatg gagcagctat ccagactcta gatgatgttg ttattacctt ggctgatgct
     4921 atcgaccctt cacctaaatt gacctctatt gcccttgtag gggggcctgc caatgacaag
     4981 gtgcagttgt cttggcgtga ggagtcgaaa ggtcgattcc gccttgagta cccggtaatg
     5041 ttcccttctt tgaaggaagg ggaatcttac acgctgactg tttccggtga ggatgcacaa
     5101 ggcaatgcgg ttcaaaaggc tgttggcttc gagtacaaac ctcgtcaggt aatgctggcg
     5161 gatgggatgg atggcaaggt catggtcccc gctgtcactc atgaatttgt tcatgcagat
     5221 ggcaaacgga tcatcgaaac caagccgctg acgctcagtg atggtgctgt cgtgacaggt
     5281 tcatacgacg tgtttgcgac cctacgttct gatgcgaaag taccgctggt ggttaatggg
     5341 gtgcgtatcg agccaggcca gacaatgggg atcatgagcc aacatgattt cggtgcgtca
     5401 ggtggtcgtt tgagcattcc ggttaaacct gctgttcctg atgtggtcgg ctcttccagc
     5461 cttcttgtca tgacctccgc gccgaactca cccatcttgg ttgtggacat caatacctgg
     5521 aaagggacgg ctaagctctc tgctgaatca tggacaattc gccaggtcat tgacccagtg
     5581 aaaatctatg ccctgccaga gtcgggtgtg ccttgccggt tcaccacgaa agaggatgtg
     5641 gctatggccg cagacccaat tcgtgacccg gtgtgtttgc tccagtggga cagaactcca
     5701 gatgaggctg aacaaactac gcaggacaac aacgggatga aagttgctgg gctggtgggg
     5761 caggctgtga gcattggtga acaacctgtc gaatacagcc tgtacctgtt cagtggtgac
     5821 ggttccaagg taaaagttgg gtctggctct cagaacctga cggtaactac tgcctatggc
     5881 tctgttggct acaccccgat tgatgacatt gctcaggtga atcgcgtcat tgaagatttt
     5941 gatgtgaact tcaagcagag caaggggcca gactgttcta taaccctcag cgcagaccgt
     6001 gcgaaaaaag aggccgcgaa caaggctgtt ggtagtgcaa gccgtgcctg tctgttcgag
     6061 tggcagcaga tccccgatgg attggtccaa gacccgttat cggaatcacc ttcgctctct
     6121 ggttcattgg cctccaacgg cgttcatcca ctggggtggc gggtaagtat tttcacccgt
     6181 aacggcacta gggtgacgtt gaacgacgag actttcaatg tcgaagcggt tgacccacca
     6241 gctccgaccg ttgaactagc ctccgactac aacttcaaag acaacatcta cttggtgccg
     6301 atgacaggta actacctggg tgatgccatt atcaactctg aacgagctga tctggatatt
     6361 gccatatcgc gcaactcgga tgttcttgag tctgagacct tcactccggg atggggtgcc
     6421 accaataagg tgtatcgccg catcaatacc gatgagcgag cgttgtggga ggagaccacc
     6481 tacaaggtga acgcggccta caacaaggtg cctgatgtga agactgaggt tgtctaccgg
     6541 gctatctctg caccttctga cagtatccgt cctatcgttg aagttaaagg tgataccgcg
     6601 attgacaccc aggcattgcc ggttagggtt ctcatccgtg atcagtacaa acctgatggt
     6661 gactatgacg ctaacacgat gggggtgtgg aaagtacgcc tgatccagca aaaggcctac
     6721 aacgagacgg ttgcgctcac tgattatgcg gaagcatcga acggtgaagc tcagttctca
     6781 gtagacctgt ctggtgtgga tacttcctcc gtccgtatcg ctgctgaggc tgttctggaa
     6841 agccctgttg agggttacaa ccgcacagag ttgtctatca gacctgcctt cttgacagtg
     6901 cttcgcggtg gtgccatcgg tgctggcgtg gaggctcgca agttgtctgg tgaagctccg
     6961 ttcactgctg tgttcaagct atctttggac gaccgtcagg atctccgggc taccggccag
     7021 gttgtgtggg aaaccagtaa ggacgacggt aaaacctggg agcagttcat cccagaagat
     7081 cgatacaagt atcagcttgt gaagaccttc gacaaggggg agtaccaggt tcgggccaag
     7141 gtggtgaacg tcaactcagg tgcggaaaag tacaccgaag cggtcagtgt tgtcgcttac
     7201 gacaaacctg atattgctgt tatcggcccg accacgttgt ttgtcggaag tgaaggcaag
     7261 tacacagcga acctgacgtt gaacgatgag ccaatctccg gtggcaatgc cattgttgag
     7321 tggtctactg acggtggcaa aacctacgcg cagacagggg atagcatcac gctttcgagc
     7381 gatgaagaaa cccgataccg cctgtgggct cgtgtgcgct ctgccacggc accggctgat
     7441 gacggctatg cctatgaagt tgcgaaaacg gcggttgact tccgagcagt gaaggcacct
     7501 cgtccttacg tgacagggcc gcgagttatt gagaccggta agaagtatgt gttcaaagcc
     7561 gaaaccagcc tgccttaccg tgggatggac gtgaagctga acgggttctt cacgctgcct
     7621 gatggctcga ttgtgcaggg tgatactgct gagtacgaac cctctgacac tgacctcaat
     7681 caggctactg tagaaacgaa gtacaccacc tggatcgaag gataccgaga tcagggtgca
     7741 gaggcttcgc atagcctacg ctcccgcgtt tggcagtatg tatggccgag cttcggaatg
     7801 caggttagga agaacgcaga cgtggctcct gcgacgatca ccgcgtcagt gcggccaatt
     7861 gccttcaacg gcaagctgga agagccgacc tacgagtggg agttgccgga aggcgctgtg
     7921 attcaggatc agcggcagga tattgtccgg tcctttgtga tcaatgagcc gggtgattac
     7981 aacatcaagg tcactgtccg tgatgctcgc ggccatgaga ccgtgatcga gcaaccgctc
     8041 aagatcggcc aggcagcgcc ctatgccatt gacctgcaat actctggctc gaacaaatac
     8101 gagcgtgagc ctctggatgt gctgttgcga ccgtacattt ctggcggcca cccacgcgac
     8161 cgtatttcga ctcgtgttta ttcggtagat ggtacaccgt tggaaagcag tggttactac
     8221 ggcagagcaa ctctgggcgc tggtgagcac agcatcaagc tgaaaataac ctcagaaatg
     8281 gggcatgaag ctgagggcga ggtgaacatc aatgttgcag agaacaagtt gcctgcatgt
     8341 agcctgagct cacgagagac cgttgggtcg tggatcgtct atgcgaactg cgaagatacc
     8401 gatggccgca tgaagtccta cgaatggacc attgccggtg agttgcagag catcagctct
     8461 gatcgagtga ctatcagcaa gggcacctat gaaacgatgc cgaccatctc tctggttggg
     8521 gtcgatgact ctgggggcaa atccgaagct gttaccatga actaaggttc attcctctca
     8581 aagcccggtt cagaccgggc ttttttttgt atcgcgctgg aattgtccaa attgggctat
     8641 tacagaggcg agtctatttc catcccccgg atacactgcc cagcatccag taatccaatc
     8701 ttaggggata gacatgcgga caaaattact tggggcgctg atggtgttcg ggattattac
     8761 cggcacggct catgcgtcat cgaaattgga aatcaccgat cccagagcgg cgaagataga
     8821 ggacattgta gagctaccca tcaaaggggt tcgagccgtc caaagtgatg ggcagatcat
     8881 gttcctctct gaaaacggac gatttgttat ttcaggacaa atctacgacc tgtggagcaa
     8941 gaagcccctc aacacgatgt cccaaatgag ggatgtagcg gagcgtatcc acttcaagag
     9001 catgggcatg gatgtggaca cgctgaacac tgtgtcgatg gggcgtggtg acaaagaggt
     9061 ggtggtcttt gttgatccta ggtgcgcggt ttgccatcag ctcatgggtg atgccaaatc
     9121 gctggtggat gattacacct ttaaatttat cgtgattcca gctctgggtg ctgagtccaa
     9181 ccgcctggca aaaaacttgt actgcgcgaa agacaaaacc cacgcgcttg atgcgctgat
     9241 gaacaacacc ctgggttccc ttccttcaaa agaaacctgc gaccccggcc aatacgacca
     9301 aacgctgctg acagctcatt tcattgggat tgagggcgtt ccgttcgttg ttgctccgga
     9361 tggtcgtgtc agcaaaggac gtccgaagaa cctgaaatca tggttggaga gtgctgaatg
     9421 atcgtaacca tcaaaaagaa actcgaagaa acgttgatcc cggagcactt gcgagctgcc
     9481 gggattattc ctgtcctggc ctatgacgaa gacgatcatg tcttccttat ggatgatcac
     9541 agtgcaggct ttggtttcat gtgtgagccc ctgtgtggtg ccgatgaaaa agttcaggag
     9601 cgaatgaacg gtttcctgaa tcaggagttc ccgtcgaaga ctacgctcca gtttgtcctg
     9661 ttccgctccc cggacatcaa tcaggagatg taccggatga tgggtttgcg tgatggcttc
     9721 cgtcacgagc tgctgacatc tgttatcaag gaacggatta acttcctcca gcaccacacg
     9781 acagatcgca tatttgccaa gaccaacaaa ggtatctacg acaatggctt gatccaagac
     9841 ctcaagctgt tcgttacgtg caaagtcccc atcaagaaca ataacccgac tgaaagcgaa
     9901 ctccagcagc tcgcacagct tcgcacgaag gtcgaatcat cgcttcaaac cgttggtctg
     9961 cgtcctcgca caatgacggc ggtgaactac atccggatca tgagcaccat cctgaattgg
    10021 gggccagatg cttcatggcg acatgactct gtggattggg agatggataa gcccatctgc
    10081 gagcaaatct tcgattacgg caccgacgtg gaagtcagct tacctgctga ttgcttcaca
    10141 cccgggctcc tcgcactgcg cgcgggttat tgtttcgttt tcagccccgg ggtcgccaag
    10201 gctctggatg gggtactact tccattgaca gcatgaggta acaatactga tggtaacgct
    10261 gaccattaac cgtaaaccac agggcatttt cagtaaatca ccggcaacgc cgctgcagga
    10321 taaaatgaac actgcacaca acgtgaaggc cagggaacgg ataccacagt tgaacgataa
    10381 acagagccct gctctgctga aaaaacaccg tggcggcata tgacaaaacg ccagcgtaaa
    10441 aaccgtaagc gtattaatcg tcttgtggaa cactggcctg agttattcaa ccgggaaaaa
    10501 ccccaaccgc gtaaagttga gatacctgac gacctgatac aggatatcgc catcagagaa
    10561 ctggcgttta gcgcaggtgc attacgcgcg gcagtagcgt cttatgtgca gtctccgcgc
    10621 tattaccgtg cgttaatggc tggcggcgcc cgttacgacc tgaaggggca gccgtgcggg
    10681 gaagtgactc cacaggaaca gaaagaggca gagacccggc taatggtgct gaatgaccga
    10741 caaaaagcca gaaaacctcg ttgactacac aactgcgctg attcactgag ctgtaatcaa
    10801 tgtacagcgc tgtaccggac gatgggtaat ggcggtcaca accggtaacc ccagagacaa
    10861 tgcgacggtt tttaacgcta catactgacc ggatgtatca ccccgggcaa cccgaaaaca
    10921 cctttcaacc tggcattgtc cgccagcgct tatcaccttc caggtctgtt gcaataaatc
    10981 cacccgttgc gaacattctt catcaaccgt ggcactggcc cgcggatggc aaatgctcgt
    11041 ctggctgagc agcaacaggc atcgtattgt tgctcctcca tgttgttccc gcaaccagcg
    11101 taataccacc ggcgaggatg gacaggcagt gtgattacgc tcagtaatac gttcgtgcac
    11161 ctgtcggtaa aaggaagtac agaatgcatg aatctgttgc ccgttgatgt ttccttctgc
    11221 cgaatagagc gtgaagtgaa atgccagcag atttggccag tggtccaccg cctgctgaac
    11281 aaaacgccgg atgtctcccg gctctgaaat taaggcttcg gttatttgca cgaaatgttc
    11341 cttttcaaac cctgttgaat tttgttaagc cggtgcagac acacgggaag gtgcgcatca
    11401 acaccggtgt aatgccagtt ataaacaatg tgaatggaat gacgaatcag tcaggaaaga
    11461 gctacaacct gctggctgac cggagagggt cagcaggaaa atgaacggta atgtcgccag
    11521 caccagggac agggctgatt tccgcaatac cagcgccggc attttgcaca ttgccagcgt
    11581 accggcatca caacacgttt catgctgctg ctcctcgtga ttcctcaatt cgctgctgca
    11641 tccactgctc cacttcactt ttgtaccagc ggctgctgcg gcccagcttg atgggtttcg
    11701 ggaaatggcc ttcactgatc agcttgtaaa accatttatc ggtcatgccg gtgaaactgg
    11761 tgatgtagtt catgtcgatc aggggatcct cggcacgcag caatacactg tcgtggcctg
    11821 ttattgaggt caacatagtc agaatatcct ctgtgagatt tatgaaggtt gggcaaatgc
    11881 gggtgaatct gctgaactga gctttcagag ctggcctgct acgggatacc agggattgcc
    11941 ggcaaggcaa cgggcttccg gatcgcacac tccacggcga tcgcgggcga aatcactgaa
    12001 cgcccagata aggtccttaa acagggcttc gcagtccggt gaccggcgat ccagtaccac
    12061 atcccgggtg atctgcttct ggtacggctt tgcttcacct ggtgacgcga aacccagcac
    12121 gttactccag gcatccagaa tcaaccgatc cagttcgggg ccgtcgaaat actcctgtct
    12181 gacgcataac accacgtggt attccggctt ttcaggatga cgggtcagga gccagcaaag
    12241 gtcacgtccg ggtgttgcat tcatcagcgc actgacggtt tcgataaaat ccataccaaa
    12301 ccaggtcgtt ctgctgttga aggtattgtc atattcacac ggcagacgaa gcaggatccg
    12361 ccgtatccgt gagccggact gctcacgcag cgcacgctca aggacgccga tgactttgtt
    12421 ctgataagat ggccagaagg gggccaggct atagtcgaaa taagggatag cattcataag
    12481 atatgctccg ttatgcgcag cccacaatgc gtcctgcccg tgaataagct gacgtgttat
    12541 ggtttgcgga tggggttgat ggaataagtg aagaaccggg gcagaacggg aagatcaggt
    12601 caccgacaag ggacagaaca ccggatgtgg ttcagagtga atgatctccg ttgacttcag
    12661 ttcacatcat taagcaaggc ggtaaggttt taccgacgga atgtttccag gacaggaagc
    12721 tcaggattta agcttcagaa gtgaggctta cgaggacgcc ctgcagcagg tcgttcagga
    12781 acttcactgc aaccgtatgc gcacagtccg tctttttgct cttctttcgc cagataatgc
    12841 agggcaccgc gaatattatc gatgctttca ggatcattgt ggcgcacagg aatgttgatg
    12901 ttgtatgtat aatgcggctg atatgtacag cggtgtgccg aaccgctgtt atgcgtaata
    12961 gaccgccagc attccgtaat ccgctccgca aagggatata tttttttaac cctctgacga
    13021 tcgagccaga aaaccgcatg cgcatgaaaa ccatgatcag ccgtccattc aatcacccag
    13081 aagaaaccaa cgatattttc aaattgttcc acctgctcca gcagcatacg caactgcaat
    13141 tcaagccagc gatgatcagg ttgtaagaaa tcaggcgtat ctttcctgta gaagaaatcc
    13201 atgcgtaagg cccgcaggcg ggaatagcgt gcgaccaact gattgatgtg attgtcaagt
    13261 aaagcggtaa aaaaccagtt tggtgtgtaa gatttaggca tgctgatgac tcctgaaagt
    13321 taatgacgtt ttaagtgctg taaacgaatt aatgctttcg ctcataatca gatctaaaac
    13381 ggtaataact tacagacggt atttatcagg catgctgtaa cagaatggat acgtcggtta
    13441 ttaacgacac gtagcgttgt gcagaaataa atggattatc tgatggtatt agctaaagag
    13501 atgagataaa cgaggagcga ataaaataca gaatcaataa acatcacaaa atactgcaaa
    13561 acacgcgata aaaacccacg accagaagtt caagaaacat attacgacca ataacatcat
    13621 aaactacaaa cttgctctca gtggttctaa aaaaaacgta tacaaggcca acacaataaa
    13681 cttatacgcc ctcatccaat ctgttcttaa ggacatgaac gttaaccgca cacattacca
    13741 gagtaaaaaa taccactggc atgattttta ccctgcatct taaacaatac aacccgaaaa
    13801 aaacataatc aggaattcac tctgttaatt tcattgttta tcctgcactg ttaaacatga
    13861 cgaaatcatg gacaccttat caaagagtaa acattcagtt aatcagtgat gacagaagta
    13921 ttgatattta ccagaatggt taaaacatat cagataaaca atcattgtta agcaaataca
    13981 gctcatgatg gataaaatta aaataaatta ttattttact gaaaacagga acagatggat
    14041 atcagcaata gcgtagtaga gaataactta tctgagtatc atatgagtta cagtagttat
    14101 gattgaggaa tggagaaggt ggataccatg ataatggtga caatgaagat tagacaagga
    14161 agggagtgaa tataactgga tgagaataag agtaatggaa tgtgtaaaga
//
LOCUS       DAPHDW010000117        14157 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.118, whole genome shotgun sequence.
ACCESSION   DAPHDW010000117 DAPHDW010000000
VERSION     DAPHDW010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14157)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14157)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14157
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.118"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(225..1109)
                     /gene="yghA"
                     /locus_tag="RH057_003402"
     CDS             complement(225..1109)
                     /gene="yghA"
                     /locus_tag="RH057_003402"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HDV3129720.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPVPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASIITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            1300..1794
                     /locus_tag="RH057_003403"
     CDS             1300..1794
                     /locus_tag="RH057_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HDV3129721.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLNWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            complement(1834..2874)
                     /gene="gpr"
                     /locus_tag="RH057_003404"
     CDS             complement(1834..2874)
                     /gene="gpr"
                     /locus_tag="RH057_003404"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HDV3129722.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMYREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDERVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            3031..3918
                     /locus_tag="RH057_003405"
     CDS             3031..3918
                     /locus_tag="RH057_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HDV3129723.1"
                     /translation="MPRLTAKDFPQELLDYYDYYAHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVV
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQAAGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     ANIEAPLLLHFAELDTRINEGWPAYEAALKANNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            4037..4324
                     /locus_tag="RH057_003406"
     CDS             4037..4324
                     /locus_tag="RH057_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HDV3129724.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            4513..5631
                     /gene="hybO"
                     /locus_tag="RH057_003407"
     CDS             4513..5631
                     /gene="hybO"
                     /locus_tag="RH057_003407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HDV3129725.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGAAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEEGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            5634..6620
                     /gene="hybA"
                     /locus_tag="RH057_003408"
     CDS             5634..6620
                     /gene="hybA"
                     /locus_tag="RH057_003408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HDV3129726.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            6610..7788
                     /gene="hybB"
                     /locus_tag="RH057_003409"
     CDS             6610..7788
                     /gene="hybB"
                     /locus_tag="RH057_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HDV3129727.1"
                     /translation="MSHDPKPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIVLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            7785..9488
                     /gene="hybC"
                     /locus_tag="RH057_003410"
     CDS             7785..9488
                     /gene="hybC"
                     /locus_tag="RH057_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HDV3129728.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            9488..9982
                     /locus_tag="RH057_003411"
     CDS             9488..9982
                     /locus_tag="RH057_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HDV3129729.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKSAPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            9975..10463
                     /gene="hybE"
                     /locus_tag="RH057_003412"
     CDS             9975..10463
                     /gene="hybE"
                     /locus_tag="RH057_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HDV3129730.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRSGE
                     NA"
     gene            10456..10797
                     /gene="hypA"
                     /locus_tag="RH057_003413"
     CDS             10456..10797
                     /gene="hypA"
                     /locus_tag="RH057_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HDV3129731.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPICHGERLRVDTG
                     DSLIVKSIEVE"
     gene            10810..11058
                     /gene="hybG"
                     /locus_tag="RH057_003414"
     CDS             10810..11058
                     /gene="hybG"
                     /locus_tag="RH057_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HDV3129732.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(11181..12047)
                     /gene="yghU"
                     /locus_tag="RH057_003415"
     CDS             complement(11181..12047)
                     /gene="yghU"
                     /locus_tag="RH057_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HDV3129733.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPVSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKHTETMNWLFWLQGAAPFLGGG
                     FGHFYHYAPMKIEYAINRFTMEAKRLLDVLDKQLAQHKFIAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            12252..14111
                     /gene="gss"
                     /locus_tag="RH057_003416"
     CDS             12252..14111
                     /gene="gss"
                     /locus_tag="RH057_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HDV3129734.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKADGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
BASE COUNT         3272 a         3567 c         3849 g         3469 t
ORIGIN      
        1 gcggaaggga tgaaaaaaac agcgcctgac ggcgctgtgt ctggcatgcc tgcaatccgg
       61 gaaaccggac caggaaaaaa cttgcaggcc ataacagtat ctacaaaatc tattgcaaca
      121 tgttgaatct tcatgcgttt gtaggcggat aaggcgtttt cgccacatca ggtaagagtg
      181 aatttacaat gatgcccggt tgcttttcac aaccgggcat ttttttaacc taaatgctcg
      241 ccgccgcaca caccgtgcac ttctgcggtg acgtagctcg actcctgact tgccagataa
      301 acatatacag gggccagttc cgccggttgc cccgcacgct tcatcggcgt tttctgacca
      361 aactgcggga tcttatcctg cgtttgtccg ccggaaattt gcagtgccgt ccagataggg
      421 cctggcgcga caatattcac ccgaatacct ttctccgcga cctgttttgc caggccacgg
      481 ctgtagttca gaatcgccgc cttcgtagcc gcatagtcca gtaaatgcgg acttggctgg
      541 tatgcctgga ttgacgaagt ggtgatgata cttgcacctt tcggtagcag ggggatcgct
      601 tcctgggtta gccagaacag cgcgaaaacg ttaatggcaa aggtcttttg aaactgttcg
      661 ctggtgaggt ctgcaatatc cggaatggca acctgtttcc cggcgaccag cgccataata
      721 tccagcccgc ctaacgcctt atgcgcttcg tgaaccagcg aacgggcgaa tttctcatcg
      781 cttaaatcgc ctggcagcag aacggctttg cgtccgcatt cttcaatgat ctttttcaca
      841 tcctgagcgt cttcttcttc cacgggaaga taactgatcg ccacgtcagc cccttcacgc
      901 gcgtaagcga tggcggcagc gcgacctatt ccggaatcgc cccctgtcac cagtgcttta
      961 cgatctttca ggcgaccgct accaacatag gttttctcgc cgcaatccgg taccggtgtc
     1021 atcttcgcct ggatgcctgg cgtcggttgt ttctgtttgg gatattcacc agtgtaatac
     1081 tgcgtggtcg ggtcttttaa atgagacatc gtttttctcc cttcaggttc aacgtccttt
     1141 aagggtagac gctctcgatg cgttgataag ggaaccagga agatccctaa acctcagaat
     1201 tatgcgacaa aggtttaacg tatatgttga tttgctgttg cgtgctgttt actcaattgc
     1261 gatatactgt tgcccgtttt aactacacga caggaatgta tggaacgttt tcttgaaaat
     1321 gcaatgtatg cttctcgctg gctgcttgcc cccgtgtact ttggcctttc gctggcgtta
     1381 gttgccctgg cgctgaagtt cttccaggag attattcacg tactgccgaa tatcttctcg
     1441 atggcggaat cagatttgat cctcgtgttg ctgtcgctgg tggatatgac gctggttggc
     1501 ggtttgctgg tgatggtgat gttttccggt tatgagaatt tcgtctcaca gctggatatc
     1561 tccgagaaca aagagaagct gaactggctg gggaaaatgg acgcaacgtc gctgaaaaac
     1621 aaagtagcag cgtcgattgt ggcaatttct tccattcacc tactgcgcgt ctttatggat
     1681 gcgaaaaatg tcccggataa caaactgatg tggtacgtca ttatccatct gacgtttgtg
     1741 ctttctgcat ttgtgatggg ctatcttgac cgactgactc gtcataatca ctgatctgat
     1801 tcgggcgcgg ttcgcgcgcc cgttattaac aggtcattta tcggacgacg cctgccacag
     1861 attcagctcg ccatcggcga tatgctgatc aatctgcgcc agctcctcgg tgctaaatgt
     1921 cagattattc agcgcctgca cgttctcctc aagttgctcc gcgcggctgg caccaatcaa
     1981 taccgacgtc actcgctcat ctttcagcaa ccaacttaac gccatttgcg ccattgattg
     2041 tccacgctgc tgtgccattt cattcaataa gcgcaggctg ttgaggttgg cttcggtaag
     2101 cattttcggt gtcagaccac gaactttatt cccttcacga tacatccgtg aatcttgcgg
     2161 aatgccgttg agatattttc cggtcagcaa tccctgagcc agaggagtaa aggcaataca
     2221 gcccacgccg ttattttgca gggtatccag caggccgctt ttatccaccc agcggttcag
     2281 taaattgtac gaaggttgat gaattaaaag cggaattttc cactcgtgca gcaactcgac
     2341 cattttttgc gtccgctctg gcgagtaaga ggagatcccg acatacagcg ccttaccgct
     2401 ttgtaccgca tgagccagcg cagaggcggt ttcttccatc ggcgtatttt catcgacgcg
     2461 atgagagtaa aagatatcga catactcaag ccccatacgc ttcaggcttt ggtcgaggct
     2521 ggcgagcagg tatttacgtg aaccgccaga gccgtaaggg ccgggccaca tatcgtagcc
     2581 agccttggta gagataatca gttcatcgcg ataagcggca aaatcctccc gcagcaggcg
     2641 accaaagttc tcttctgcgc ttcctggagg cggcccgtaa ttgttggcta aatcaaagtg
     2701 cgtaatgccc aaatcaaacg ctttacgcag gatcgcacgc tgtgattcca gcgcgttaac
     2761 gtgaccgaaa ttgtgccata aaccgagcga taacgcgggc aggcgtaaac cgctttttcc
     2821 gcaatagcgg tattgcatct gcccgtaacg ttcgggattc gctaaccaga ccatgacctc
     2881 tcctttccac cgttcaattt cgaaacaatg tttctagttt agcgattcgc cagcgcgtat
     2941 cccgtagtct ggctcacaga gtgacgaaaa actgggcaaa aacacgtgct tatgctttgc
     3001 ttaaaaaaac accagttgag gagtgcaacg atgccgcgtt taaccgccaa agatttccca
     3061 caagagttgt tggattacta cgactattac gctcacggga aaatctcgaa acgtgagttc
     3121 ctcaaccttg cggcgaagta tgcggtgggc gggatgacgg cattagcgtt gtttgatttg
     3181 ctcaagccaa attatgcgct ggcgactcag gtagagttta ccgacccgga gattgttgct
     3241 gagtacatca cgtatccttc gccaaatggt cacggcgagg tacggggtta tctggtgaaa
     3301 cccgcaaaaa tgagcggcaa aacgccagcc gtggtagtgg tgcatgagaa tcgtggactg
     3361 aatccgtata tcgaagatgt ggcacggcga gtggcgaagg cggggtatat cgccctggca
     3421 cctgacggct taagttccgt tggaggttat ccgggaaatg atgataaagg tcgtgagctg
     3481 caacagcagg ttgatccaac caaactgatg aatgatttct ttgccgcaat tgagtttatg
     3541 caacgctatc cgcaagcggc aggcaaagtg ggtattaccg gattttgcta tggcggtggc
     3601 gtatcgaacg cggcggctgt cgcgtatccg gaactggcct gcgcggtgcc gttttatggt
     3661 cgtcaggcac ccactgccga tgtggcgaat attgaagcgc ctttactact ccacttcgcg
     3721 gaactggaca cccgaatcaa cgagggctgg cctgcttacg aggcggcgtt gaaagccaat
     3781 aataaggttt atgaggcgta tatctatccg ggggttaatc acggattcca taatgattcc
     3841 acgccccgtt atgacaaatc tgccgccgat cttgcctggc aaaggacact gaaatggttc
     3901 gataaatatc tctcctgata ggtttatctc ttactggatt acgtcttaaa caagcatgaa
     3961 aaaatagcgt gcgcaaaagt cgttctttgc ctaaaatatc gctatatatg gcgatatata
     4021 gcgaatgagg tgaacgatga ataaccattt tggtaaaggc ttaatggcgg gattaaaagc
     4081 aacgcatgcc gacagtgcgg ttaatgtgac aaaattctgt gccgattata aacgcggttt
     4141 tgtattaggc tactcacacc ggatgtacga aaagaccgga gatcgccagc ttagcgcctg
     4201 ggaagcgggt attctgacgc gccgctatgg actggataaa gagatggtaa tggatttctt
     4261 tcgtgagaat aattcctgtt ctacgttgcg cttttttatg gccggttatc gcctcgaaaa
     4321 ttgatcaaac atacgtatta tcttgcttta attaattaca ctaatgcttc ttcccttcgt
     4381 tttagcgccc cgccgcagta tcatgatatc gataaccata ataaatgtgt ggtaaatggc
     4441 gcatcgatcg cattattgat tttgcgattg aggcaaaata tatgccaggt cttcgcaacg
     4501 gaataactat aaatgactgg agataacacc ctcatccatt ctcacggcat taaccgtcgt
     4561 gatttcatga agctttgtgc agcattagcc gccaccatgg ggttaagtag caaagccgct
     4621 gcagagatgg ccgaatcggt tactaacccg cagcgtccgc cagttatctg gattggcgcg
     4681 caggagtgca ccggttgtac ggaatctctg cttcgtgcaa cgcatccaac ggtagaaaac
     4741 ctcgtgctgg agactatctc tctggagtat cacgaagtgc tttccgccgc cttcggtcat
     4801 caggtcgaag agaacaaaca taacgcactt gagaagtaca aagggcagta tgtgttggtt
     4861 gtggatggtt ccatcccatt aaaagataac ggtatttatt gcatggttgc tggtgagccg
     4921 attgtggatc acatccgcaa agcggcagaa ggcgcagcag cgattatcgc tatcggttcc
     4981 tgctctgcgt ggggcggtgt tgccgcagct ggagttaacc caactggcgc agtcagcctg
     5041 caagaagttc tgccaggcaa aaccgttatc aatattccgg gctgcccgcc gaacccgcac
     5101 aacttcctcg ctaccgttgc gcacatcatc acttacggca aaccgccgaa actggatgac
     5161 aaaaatcgtc cgaccttcgc ctatggccgt ctgattcacg aacactgcga acgtcgcccg
     5221 cacttcgatg ctggtcgttt tgccaaagag ttcggtgatg aaggccaccg tgaaggctgg
     5281 tgcctgtacc acctcggctg taaagggcca gaaacttacg gcaactgctc aacgctgcaa
     5341 ttctgcgatg ttggcggtgt gtggccggtg gcgattggtc acccgtgcta tggctgtaac
     5401 gaagaaggta tcggcttcca taaaggcatc catcagcttg ccaacgtcga aaatcaaact
     5461 ccgcgttcac agaaaccgga tgttaacgct aaagagggtg gcaacgtctc tgcaggcgct
     5521 attggtttgc tcggcggtgt ggttgggttg gttgccggtg tcagcgtgat ggcggtgcgt
     5581 gaactgggtc gtcagcaaaa gaaagataac gctgactcac ggggagaata accgtgaaca
     5641 gacgtaattt tattaaagca gcctcctgcg gggcattgct gacgggcgcg ctgccgtctg
     5701 tcagtcatgc ggctgctgaa aaccgcccgc caattccggg atcgctgggg atgttgtacg
     5761 actcgacctt gtgcgtaggc tgccaggctt gcgtcaccaa gtgtcaggat atcaatttcc
     5821 ctgaacgtaa cccgcaaggg gaacagacct ggtcgaacaa cgacaaactg tcgccgtata
     5881 ccaataacat cattcaggtg tggaccagcg gcacaggggt caacaaagac caggaggaga
     5941 acggctacgc gtacattaag aaacagtgta tgcactgcgt cgatccgaac tgtgtctctg
     6001 tgtgcccggt ctctgcactg aaaaaagatc cgaaaaccgg cattgtccat tacgacaaag
     6061 acgtgtgcac cggttgccgt tactgcatgg tcgcctgtcc gtacaacgtg ccgaagtacg
     6121 actacaacaa cccgtttggt gcgctgcata agtgcgagct gtgcaaccag aaaggtgtgg
     6181 aacgtctcga taaaggcggt ctgcctggct gcgtagaagt gtgcccggcg ggcgcggtga
     6241 ttttcggtac gcgtgaagag ctgatggcgg aggcgaaaaa acgtctggcg ctgaagcctg
     6301 gcagcgaata ccactatccg cgtcagacgc tgaaatctgg cgacacttac ctgcatacgg
     6361 tgccgaaata ttatccgcat ctgtacggcg agaaagaggg cggcggtact caggttctgg
     6421 tactgacggg tgtgccttat gaaaatctcg acctgccgaa actggacgat ctttctaccg
     6481 gtgcgcgttc cgaaaatatt caacacaccc tgtataaagg catgatgcta ccactggctg
     6541 tgctggcggg cttaaccgtg ctggttcgtc gcaacaccaa aaacgaccat cacgacggag
     6601 gagacgatca tgagtcatga tccaaaaccg ctgggcggca aaatcatcag taaaccggtc
     6661 atgatttttg gaccgttaat cgtcatctgt atgctcctga ttgtgaagcg tctggtgttc
     6721 ggtctgggct ctgtctccga cctgaacggc ggctttccgt ggggcgtgtg gatcgcgttt
     6781 gacctgctga tcggcactgg ctttgcctgt ggcggctggg cgctggcgtg ggcggtatac
     6841 gtctttaacc gtgggcaata ccatccgctg gtgcgtccgg cgctgttggc gagtctgttt
     6901 ggttactcac tgggtggctt gtcgatcact atcgacgtgg gtcgctactg gaacctgccg
     6961 tacttctaca ttccgggtca cttcaacgtg aactcggtac tgttcgagac ggcggtctgt
     7021 atgaccatct acatcggcgt gatggctctg gagtttgctc cggcactgtt tgaacgtctg
     7081 ggctggaagg tgtcgctaca gcgtctgaac aaggtgatgt tcttcatcat cgcgctcggt
     7141 gcgttgctgc cgaccatgca ccagtcttca atggggtcgc tgatgatctc ggcgggctac
     7201 aaggtgcatc cgctgtggca gagctatgaa atgttgccgc tgttctcgct gctgacggcg
     7261 ttcatcatgg gcttctcgat tgtcatcttt gaaggttcac tggtgcaggc gggtctgcgt
     7321 ggcaacggtc cggatgaaaa gagcctgttc gtcaagctga ccaacaccat cagtgtgttg
     7381 ctggcgattt tcatcgtgct gcgctttggc gagcttatct atcgcgacaa gctgtcgtta
     7441 gcgtttgccg gtgacttcta ctccgtgatg ttctggattg aagtcctgct gatgctcttc
     7501 ccgctggtcg ttctgcgtgt ggcgaagctg cgtaatgatt cccgcatgct gttcctgtca
     7561 gcactgagcg cactgttagg ttgtgcaacc tggcgtctga cctattcgct ggtggcattc
     7621 aacccgggcg gcggttacgc ctacttcccg acctgggaag aactgttgat ttctattggt
     7681 tttgtggcta ttgagatttg cgcttacatc gtactcattc gtctactgcc gatacttcct
     7741 cctttaaaac aaaacgatca taatcgtcat gaggcgagca aagcatgagc cagagaatta
     7801 ctattgatcc ggtaacccgt attgaggggc atttacgcat cgattgcgaa atcgaaaatg
     7861 gcgtcgtttc gaaagcatgg gcttccggta ccatgtggcg cggcatggaa gagatcgtga
     7921 aaaaccgcga tccgcgcgat gcatggatga ttgtgcaacg tatctgtggc gtatgtacta
     7981 ccactcacgc gctgtcttcc gttcgtgcgg cagaaagtgc gctgaatatc gacgttccgg
     8041 ttaacgcgca atacatccgt aacatcattc tggctgcgca caccacgcat gaccatatcg
     8101 ttcatttcta tcagctttcg gcgctggact gggtggatat cacttctgca ctgcaagctg
     8161 acccaaccaa agcctccgaa atgctgaaag gcgtttcgac ctggcatctg aacagcccgg
     8221 aagagttcac caaagttcag aacaagatca aagatctggt tgccagcggt cagttgggta
     8281 ttttcgctaa tggctactgg ggtcacccag cgatgaaact gccgccggaa gtgaacctga
     8341 ttgcggtagc gcactacctg caggcgctgg agtgccagcg tgacgctaac cgcgtcgtgg
     8401 cgctgctggg cggtaaaacg ccgcacattc agaacctggc agtgggtggt gtcgcgaacc
     8461 caatcaacct cgacgggttg ggcgtgttga accttgagcg cctgatgtac atcaagtctt
     8521 tcatcgacaa actgagcgac tttgttgagc aggtttataa ggttgatacc gcggttatcg
     8581 ccgcgttcta cccggaatgg ctgacgcgcg gtaaaggtgc ggtgaactac ctgagcgtgc
     8641 cggaattccc gaccgacagc aaaaacggca gcttcctgtt cccgggcggc tacattgaga
     8701 atgcggatct gtcctcgtat cgtccgatca cttctcattc cgatgaatac ctgatcaaag
     8761 gcattcagga aagcgcgaag cactcctggt ataaagacga agcgccacag gcgccgtggg
     8821 aaggtaccac cattccggct tatgatggtt ggtctgacga cgggaaatat tcctgggtga
     8881 aatcaccgac tttctacggc aaaacggtag aagtgggtcc gctggctaat atgctggtga
     8941 aactggcggc aggtcgagaa tctacccaga acaaactgaa tgaaatcgtt gcgatttatc
     9001 agaaactgac tggtaacacg ctggaagtgg cgcaactgca ctctacgctg ggccgtatta
     9061 ttggtcgtac cgttcactgc tgcgaattgc aggatatcct gcaaaaccaa tacagtgcac
     9121 tgatcaccaa tatcggcaaa ggcgatcaca ccaccttcgt gaaaccgaac attccggcaa
     9181 cgggtgagtt caaaggtgtt ggcttcctcg aagcgccgcg cggtatgctc tctcactgga
     9241 tggtgatcaa agacggtatc atcagcaact accaggcagt tgttccatca acctggaact
     9301 ctggtccgcg taacttcaat gatgacgtcg gtccttacga gcagtcgctg gtgggtacac
     9361 cggttgctga tccgaataaa ccgctggaag tggtgcgtac cattcactcc ttcgacccgt
     9421 gcatggcctg tgcggtacac gtagtggatg ctgacggcaa cgaagtggtt tcagtgaagg
     9481 ttctgtaatg cgtattttag tcttaggggt cggcaatatt ttgctgaccg atgaagccat
     9541 cggtgtgcgg attgttgaag cgttagagca acgatacatt ctgccggatt atgttgagat
     9601 cctcgatggc ggcacggcgg gaatggagct gcttggcgac atggcaaatc gcgatcatct
     9661 gattattgcg gatgccattg tgtcgaagaa gagcgcgccg ggaacgatga tgatcctgcg
     9721 ggatgaagaa gttccggcgt tgtttaccaa caaaatctct ccgcatcagc ttggcctggc
     9781 cgacgtcttg tcggccctgc gcttcaccgg cgagtttccg aaaaagctga ccctggtcgg
     9841 cgtgatcccg gaatcgctgg agccacacat cggcttaacg ccgacggttg aagcaatgat
     9901 tgaacctgcg cttgagcagg ttctggctgc gctgcgcgaa tcaggcgtgg aagccatccc
     9961 acgggaggcg attcatgact gaagagatag caggtttcca gacctccccg aaggcgcaag
    10021 tacaggcagc gtttgaagaa attgcccggc gttcgatgca cgatctctct tttctgcatc
    10081 cttcaatgcc ggtgtatgtt tctgatttta cgctgttcga aggtcagtgg acggggtgtg
    10141 tgatcacccc gtggatgctg agtgcagtta tcttccccgg cccggatcaa ctctggccgc
    10201 tgcgcaaagt gagtgaaaaa attggtctgc aactgccgta tggcaccatg acctttaccg
    10261 ttggcgaact ggacggtgtt tcgcaatacc tctcctgttc gctgatgtca ccgctttcgc
    10321 acagtatgtc gattgaagag ggccaacgcc tgacggatga ctgcgcacga atgatccttt
    10381 cgctgccagt cacgaatccg gatgtaccac acgcagggcg tcgcgccctg ctgtttggtc
    10441 gcaggagtgg cgaaaatgca tgagttgtcg ctttgccaga gcgccgttga aattatccaa
    10501 cggcaggcgg agcagcacga tgttaagcgc gtcaccgccg tgtggctgga aattggcgcg
    10561 ctctcctgcg ttgaggagag cgccgtccgt tttagttttg aaattgtctg ccacggaacg
    10621 gtggcgcaag ggtgcgattt acatatcgtc tataaacccg cccaggcctg gtgctgggat
    10681 tgcagccagg tggtggagat tcatcagcac gatgcgcagt gtccgatctg tcatggcgag
    10741 cggttgcgtg tcgataccgg cgattcgctg atcgtcaaaa gtattgaagt tgaataaccg
    10801 gagttaataa tgtgtattgg cgttccaggc caggtgctgg ctgtcggtga agatattcac
    10861 cagcttgcgc aggttgaagt atgtggtatc aagcgcgacg tgaatatcgc cctgatttgt
    10921 gaaggtaacc ctgccgatct gctgggccag tgggtgctgg tacacgtcgg atttgccatg
    10981 agcatcatcg acgaagatga agccaaagcc acattagacg cactgcgcca aatggattac
    11041 gacattacca gcgcgtgatg attagctttc gtaggcctga taagacgcgg cagcgtcgca
    11101 tcaggcattg tgcacgattg ccggatgcgg cgtgaacgcc ttatccggcc tggatggctt
    11161 gctgcgacga acaccaaccc ttacccctga cgcttatctt ccgtattcgt ctcgaaatca
    11221 ctggcgtcat ggcgctcatg caactgctca ttcagcggtc cgttggtgcg gttaacaata
    11281 cgcccacgtt tcaccgccgg acgttcgcct acttctttcg cccagcgttg tacatgctta
    11341 taactgcccg catcaagaaa ctctgcggca tcatacacgc cgcctaacac cacattgcca
    11401 aaccacggcc aaatcgccat atccgcaatg gtgtactcat cgcccgcaat aaacttatgc
    11461 tgcgccagtt gcttatccag cacgtcgagc agacgtttgg cttccatggt aaagcggttg
    11521 atggcgtact caattttcat cggcgcgtaa tggtaaaagt gaccaaaacc accgccgagg
    11581 aacggtgccg cgccctgtaa ccagaacagc cagttcatcg tttcagtatg ctttgccaaa
    11641 tcctgcggca ggaagtagcc aaatttctcc gccagataaa gcaggatcga accagattca
    11701 aacacgcgga tcggcggatt atgcgtatga tcgcgcagcg ccgggatctt cgagtttggg
    11761 ttcacttcga caaagccgct ggagaattga tcgccatcgc caatacgaat cagccaggcg
    11821 tcgtactctg caccagtaac gcccagcgcc agcagctcct caagcataat cgttactttc
    11881 tgaccgttcg gcgttcccag cgaataaagt tgcaatgggt gtttgccaac gggcagcgtt
    11941 ttttcatgcg tcggaccaga aaccgggcga ttgatattgg cgaacgcgcc gccagcggat
    12001 ttatcccacg tccagacttt cgcgggctga taagtattgt ctgtcatagt gagttgcctt
    12061 ctgagtggta gtgttgaagc agtgtagcag gtcacttgct gtacagaatg tgaatttttt
    12121 aaccctttgc gccagccgct gcgatgaggt gtatgattag tctgacctct gtccggttaa
    12181 tgtcgctgag caggtaatca gagcacaaca attacgccaa ctgcttaggt tgtattgtta
    12241 aaggtaaagt gatgagcaaa ggaacgacca gccaggatgc cccgttcggg acattattgg
    12301 gctacgcccc aggtggggta gcaatctact cttcagatta cagttctctc gatccgcagg
    12361 aatacgaaga tgacgccgta ttccgtagct atatcgacga cgaatatatg ggccacaagt
    12421 ggcaatgcgt tgaatttgct cgccgttttc tctttctgaa ttacggtgtg gtctttactg
    12481 acgtgggtat ggcgtgggag attttctcgc tgcgcttcct gcgagaagtg gttaatgaca
    12541 acatcctgcc attgcaggca tttcctaacg gctcgccgcg tgcgccggtc gcgggtgcgc
    12601 ttcttatctg ggataaaggc ggtgaattta aagacactgg ccatgtcgcc atcattaccc
    12661 aattgcatgg caacaaagtc cgtattgcgg aacagaacgt gattcattcc ccgttgccgc
    12721 aagggcaaca gtggacgcgt gagctggaga tggtggtcga aaacggctgc tataccctga
    12781 aagacacttt tgatgacacc accattctgg gctggatgat ccagacggaa gatactgaat
    12841 acagcttacc gcagccggaa attgcaggcg agctgctgaa aatcagcgga gcgcgcctgg
    12901 aaaacaaagg ccagtttgac ggtaaatggc tggatgaaaa agatccgctg caaaacgcct
    12961 atgtgcaggc caacggtcag gtgatcaatc aggatcctta tcattactac accattaccg
    13021 agagtgccga gcaggagcta attaaagcca ccaacgagct gcacctgatg tatcttcacg
    13081 caaccgacaa ggtgctgaaa gatgacaacc tgctggcgct gttcgacatc ccgaaaatcc
    13141 tctggccacg tttgcgtctc tcctggcagc gtcgccgtca ccatatgatc actggtcgta
    13201 tggatttctg catggatgag cgtggcctga aggtttacga gtacaacgcc gactccgcct
    13261 cctgtcatac cgaagcgggc ttgatcctcg aacgttgggc ggagcagggc tataaaggca
    13321 acggcttcaa tccggcggaa gggctgatta acgaactggc tggtgcctgg aaacacagtc
    13381 gtgcacgtcc gtttgtccat atcatgcagg acaaagatat cgaggaaaac tatcacgcgc
    13441 agtttatgga gcaggcgctg caccaggcgg gctttgaaac gcgtatcttg cgcgggctgg
    13501 atgaactggg ctgggatgct gccgggcaac tgattgatgg ggaaggacgg ctggttaact
    13561 gcgtgtggaa aacctgggcg tgggaaaccg cgtttgatca gattcgtgaa gttagcgacc
    13621 gtgagtttgc tgcggtgcca atccgtaccg gtcatccgca aaacgaagtg cgtcttatcg
    13681 acgtattgct gcgcccggaa gtgctggtct ttgagccgct gtggacggtg atccccggca
    13741 acaaagcgat tctgccgatc ctctggtcgc tgttcccgca ccatcgttac ctgctggata
    13801 ccgatttcac tgttaatgat gaattggtga aaacaggtta cgcagtgaaa ccgattgccg
    13861 gtcgctgtgg tagcaatatc gacctggtca gccatcatga agaggtgctg gacaaaacca
    13921 gcggtaaatt tgccgagcag aaaaacatct atcagcaact gtggtgtttg ccgaaagcgg
    13981 acggtaaata cattcaggta tgtaccttca ccgttggcgg caactacggt gggacgtgtt
    14041 tgcgcggtga tgaatcactg gtcatcaaaa aagagagtga tattgaaccg ttaattgtgg
    14101 tgaaaaagta atacatgttg tagcaacccc gtactgatta tgaagaataa tcagtac
//
LOCUS       DAPHDW010000118        14129 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.119, whole genome shotgun sequence.
ACCESSION   DAPHDW010000118 DAPHDW010000000
VERSION     DAPHDW010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14129
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.119"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            99..641
                     /gene="ecpR"
                     /locus_tag="RH057_003417"
                     /gene_synonym="matA"
     CDS             99..641
                     /gene="ecpR"
                     /locus_tag="RH057_003417"
                     /gene_synonym="matA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECP biosynthesis operon DNA-binding
                     transcriptional regulator EcpR"
                     /protein_id="HDV3129735.1"
                     /translation="MECQNRSDKYIWSPHDAYFYKGLSELIVDIDRLIYLSLEKIRKD
                     FVFINLNTDSLSEFINRDNEWLSAVKGKQVVLIAARKSEALANYWYYNSNIRGVVYAG
                     LSRDIRKELAYVINGRFLRKDIKKDKITDREMEIIRMTAQGMQPKSIARIENCSVKTV
                     YTHRRNAEAKLYSKIYKLVQ"
     gene            716..1303
                     /gene="ecpA"
                     /locus_tag="RH057_003418"
                     /gene_synonym="matB"
     CDS             716..1303
                     /gene="ecpA"
                     /locus_tag="RH057_003418"
                     /gene_synonym="matB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="common pilus major fimbrillin subunit EcpA"
                     /protein_id="HDV3129736.1"
                     /translation="MKKKVLAIALVTVFTGMGVAQAADVTAQAVATWSATAKKDTTSK
                     LVVTPLGSLAFQYAEGIKGFNSQKGLFDVAIEGDSTATAFKLTSRLITNTLTQLDTSG
                     STLNVGVDYNGAAVEKTGDTVMIDTANGVLGGNLSPLANGYNASNRTTAQDGFTFSII
                     SGTTNGTTAVTDYSTLPEGIWSGDVSVQFDATWTS"
     gene            1361..2029
                     /gene="ecpB"
                     /locus_tag="RH057_003419"
                     /gene_synonym="yagY"
     CDS             1361..2029
                     /gene="ecpB"
                     /locus_tag="RH057_003419"
                     /gene_synonym="yagY"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpB"
                     /protein_id="HDV3129737.1"
                     /translation="MKKHLLPLALLFSGISPAQALDVGDISSFMNSDSSTLSKTIKNS
                     TDSGRLINIRLERLSSPLDDGQVISMDKPDELLLTPASLLLPAQASEVIRFFYKGPAD
                     EKERYYRIVWFDQALSDAQRDNANRSAVATASARIGTILVVAPRQANYHFQYANGSLT
                     NTGNATLRILAYGPCLKAANGKECKENYYLMPGKSRRFTRVDTADNKGRVALWQGDKF
                     IPVK"
     gene            2055..4580
                     /gene="ecpC"
                     /locus_tag="RH057_003420"
                     /gene_synonym="yagX"
     CDS             2055..4580
                     /gene="ecpC"
                     /locus_tag="RH057_003420"
                     /gene_synonym="yagX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher EcpC"
                     /protein_id="HDV3129738.1"
                     /translation="MPLRRFSPGLKAQFAFGMVFLFVQPDASAADISAQQIGGVIIPQ
                     AFSQALQDGMSVPLYIHLAGSQGRQDDQRIGSAFIWLDDGQLRIRKIQLEESEDNASV
                     SEQTRQQLMALANAPFNEALTIPLTDNAQLDLSLRQLLLQLVVKREALGTVLRSRSED
                     IGQSSVNTLSSNLSYNLGVYNNQLRNGGSNTSSYLSLNNVTALREHHVVLDGSLYGIG
                     SGQQDSELYKAMYERDFAGHRFAGGMLDTWNLQSLGPMTAISAGKIYGLSWGNQASST
                     IFDSSQSATPVIAFLPAAGEVHLTRDGRLLSVQNFTMGNHEVDTRGLPYGIYDVEVEV
                     IVNGRVISKRPQRVNKLFSRGRGVGAPLAWQVWGGSFHMDRWSENGKKTRPAKESWLA
                     GASTSGSLSTLSWAATGYGYDNQAVGETRLTLPLGGAINVNLQNMLASDSSWSSIGSI
                     SATLPGGFSSLWVNQEKTRIGNQLRRSDADNRAIGGTLNLNSLWSKLGTFSISYNDDR
                     RYNSHYYTADYYQNVYSGTFGSLGLRAGIQRYNNGDSNANTGKYIALDLSLPLGNWFS
                     AGMTHQNGYTMANLSARKQFDEGTIRTVGANLSRAISGDTGDDKTLSGGAYAQFDARY
                     ASGTLNVNSAADGYINTNLTANGSVGWQGKNIAASGRTDGNAGVIFNTGLEDDGQISA
                     KINGRIFPLNGKRNYLPLSPYGRYEVELQNSKNSLDSYDIVSGRKSHLTLYPGNVAVI
                     EPEVKQMVTVSGRIRAEDGTLLANARINNHIGRTRTDENGEFVMDVDKKYPTIDFRYS
                     GNKTCEVALELNQARGAVWVGDVVCSGLSSWAAVTQTGEENES"
     gene            4570..6213
                     /gene="ecpD"
                     /locus_tag="RH057_003421"
     CDS             4570..6213
                     /gene="ecpD"
                     /locus_tag="RH057_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial adhesin EcpD"
                     /protein_id="HDV3129739.1"
                     /translation="MRVNLLITMIIFALIWPVTELRAAVSKTTWADAPAREFVFVENN
                     SDDNFFVTPGGALDPRLTGANRWTGLKYTGSGTIYQQSLGYIDNGYNTGLYTNWKFDM
                     WLENSPVSSPLTGLRCINWYAGCNMTTSLILPQTTDASGFYGATVTSGGAKWMHGMLS
                     DAFYQYLQQMPVGSSFTMTINACQTSVNYDASSGARCKDQATGNWYVRNVTHTKAANL
                     RLINTHSLAEVFINSDGVPTLGEGNADCRTQTIGSLSGLSCKMVNYTLQTNGLSNTSI
                     HIFPAIANSSLASAVGAYDMQFSLNGSSWKPVSNTAYYYTFNEMKSADSIYVFFSSNF
                     FKQMVNLGISDINTKDLFNFRFQNTTSPESGWYEFSTSNTLIIKPRDFSISIISDEYT
                     QTPSREGYVGSGESALDFGYIVTTSGKTAADEVLIKVTGPAQVIGGRSYCVFSSDDGK
                     AKVPFPATLSFITRNGATKTYDAGCDDSWRDMTDALWLTTPWTDISGEVGQMDKTTVK
                     FSIPMDNAISLRTVDDNGWFGEVSASGEIHVQATWRNIN"
     gene            6182..6892
                     /gene="ecpE"
                     /locus_tag="RH057_003422"
     CDS             6182..6892
                     /gene="ecpE"
                     /locus_tag="RH057_003422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone EcpE"
                     /protein_id="HDV3129740.1"
                     /translation="MFRRRGVTLTKALLTAVCMLAAPLTQAISVGNLTFSLPSETDFV
                     SKRVVNNNKSARIYRIAISAIDSPGSSELRTRPVDGELLFAPRQLALQAGESEYFKFY
                     YHGPRDNRERYYRVSFREVPTRNQTRRSPTGGEVSTEPVVVMDTILVVRPRQVQFKWS
                     FDKVTGTVSNTGNTWFKLLIKPGCDSTEEEGDAWYLRPGDVVHQPELRQPGNHYLVYN
                     DKFIKISDSCPAKPPSAD"
     gene            7205..7534
                     /locus_tag="RH057_003423"
     CDS             7205..7534
                     /locus_tag="RH057_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HDV3129741.1"
                     /translation="MSNLNPCMTCGACCAFFRVSFYWAEADDAGGTIPARLTEQISPF
                     HRCMSGTNQKNPRCIALAGTPGKNACCTIYKNRSSTCREFAMSGENGVVNEACNRARA
                     KYGLTPL"
     gene            complement(7782..8396)
                     /locus_tag="RH057_003424"
     CDS             complement(7782..8396)
                     /locus_tag="RH057_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HDV3129742.1"
                     /translation="MNIFEQTPPNRRRYGLAAFIGLIAGVVSAFVKWGAEVPLPPRSP
                     VDMFNAACGPESLIRAAGQIDCSRNFLNPPYIFLRDWLGLTDPNAAVYTFAGHVFNWV
                     GVTHIIFSIVFAVGYCVVAEVFPKIKLWQGLLAGALAQLFVHMISFPLMGLTPPLFDL
                     PWYENVSEIFGHLVWFWSIEIIRRDLRNRITHEPDPEIPLGSNR"
     gene            8814..9503
                     /gene="paoA"
                     /locus_tag="RH057_003425"
     CDS             8814..9503
                     /gene="paoA"
                     /locus_tag="RH057_003425"
                     /EC_number="1.2.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase iron-sulfur subunit"
                     /protein_id="HDV3129743.1"
                     /translation="MSNQGEYPEDNRVGKHEPHDLSLTRRDLIKVSAATAATAVVYPH
                     STLAASVPAATPAPEIMPLTLKVNGKTEQLEVDTRTTLLDALRENLHLIGTKKGCDHG
                     QCGACTVLVNGRRLNACLTLAVMHQGAEITTIEGLGSPDNLHPMQAAFIKHDGFQCGY
                     CTSGQICSSVAVLKEIQDGIPSHVTVDLVSAPETTADEIRERMSGNICRCGAYANILA
                     AIEDAAGEIKS"
     gene            9500..10456
                     /gene="paoB"
                     /locus_tag="RH057_003426"
     CDS             9500..10456
                     /gene="paoB"
                     /locus_tag="RH057_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase FAD-binding subunit
                     PaoB"
                     /protein_id="HDV3129744.1"
                     /translation="MKAFTYERVNTPAEAALSAQRVPGAKFIAGGTNLLDLMKLEIET
                     PTHLIDVNGLGLDKIEVTDAGGLRIGALVRNTDLAAHERVRRDYAVLSRALLAGASGQ
                     LRNQATTAGNLLQRTRCPYFYDTNQPCNKRLPGSGCAALEGFSRQHAVVGVSEACIAT
                     HPSDMAVAMRLLDAVVETITPEGKTRSITLADFYHPPGKTPHIETALLPGELIVAVTL
                     PPPLGGKHIYRKVRDRASYAFALVSVAAIIQPDGSGRVALGGVAHKPWRIEAADAQLS
                     QGAQAVYDTLFASAHPTAENTFKLLLAKRTLASVLAEARAQA"
     gene            10453..12651
                     /gene="paoC"
                     /locus_tag="RH057_003427"
     CDS             10453..12651
                     /gene="paoC"
                     /locus_tag="RH057_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde oxidoreductase molybdenum-binding
                     subunit PaoC"
                     /protein_id="HDV3129745.1"
                     /translation="MKFDKPAGENPIDQLKVVGRPHDRIDGPLKTTGTARYAYEWHEE
                     APNAAYGYIVGSAIAKGRLTALDTDAAQKAPGVLAVITASNAGALGKGDKNTARLLGG
                     PTIEHYHQAIALVVAETFEQARAAASLVQAHYRRNKGAYSLADEKQAVNQPPEDTPDK
                     NVGDFDGAFTSAAVKIDATYTTPDQSHMAMEPHASMAVWDGNKLTLWTSNQMIDWCRT
                     DLAKTLKVPVENVRIISPYIGGGFGGKLFLRSDALLAALAARAVKRPVKVMLPRPSIP
                     NNTTHRPATLQHLRIGADQSGKITAISHESWSGNLPGGTPETAVQQSELLYAGANRHT
                     GLRLATLDLPEGNAMRAPGEAPGLMALEIAIDELAEKAGIDPVEFRILNDTQVDPADP
                     TRCFSRRQLIECLRTGADKFGWKQRNATPGQVRDGEWLVGHGVAAGFRNNLLEKSGAR
                     VHLEQNGTVTVETDMTDIGTGSYTILAQTAAEMLGVPLEQVAVHLGDSSFPVSAGSGG
                     QWGANTSTSGVYAACMKLREMIASAVGFDPEQSQFADGKITNGTRSATLHEATAGGRL
                     TAEESIEFGTLSKEYQQSTFAGHFVEVGVHSATGEVRVRRMLAVCAAGRILNPKTARS
                     QVIGAMTMGMGAALMEELAVDDRLGYFVNHDMAGYEVPVHADIPKQEVIFLDDTDPIS
                     SPMKAKGVGELGLCGVSAAIANAVYNATGIRVRDYPITLDKLLDKLPDVV"
     gene            12661..13617
                     /gene="paoD"
                     /locus_tag="RH057_003428"
     CDS             12661..13617
                     /gene="paoD"
                     /locus_tag="RH057_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor insertion chaperone PaoD"
                     /protein_id="HDV3129746.1"
                     /translation="MSYPLFDKDEHWHKPEQAFLTDDHRTILRFAVEALMSGKGAVLV
                     TLVEIHGGAARPLGAQMVVREDGRYCGFVSGGCVEAAAAFEALEMMGSGRDREIRYGE
                     GSPWFDIVLPCGGGITLMLHKLRSAQPLLAVLNRLEQRKPAGLRYDPQAQSLVCLPTQ
                     TRTGWNLNGFEVGFRPCVRLMIYGRSLEAQATASLAAATGYDSHIFDLFPASASAQID
                     TDTAVILLCHDLNRELPALQAAREAKPFYLGALGSHRTHTLRLQKLHELGWSREETAQ
                     IRAPVGIFPKARDAHTLALSVLAEIASVRLHHEEDSCLPPSS"
     gene            13668..14006
                     /locus_tag="RH057_003429"
                     /pseudo
     CDS             13668..14006
                     /locus_tag="RH057_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014831000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT         3503 a         3769 c         3776 g         3081 t
ORIGIN      
        1 tttactaagg aaactgaatg cacctgtaaa aattacaggt ttggaaagta gtgacatggc
       61 aaagtgatta cagcagggac tatgaggtta aaaaccatat ggaatgtcaa aaccgttctg
      121 ataaatacat ctggtctccc catgacgcct acttctataa aggactatct gaactgattg
      181 tggatatcga cagattaatt tatctatcgt tggagaaaat tagaaaagat ttcgtgttta
      241 tcaatctcaa tacggattct ttatctgaat ttataaaccg tgataatgaa tggttatccg
      301 cggtaaaggg gaaacaggtc gtattgattg cggccagaaa gtcagaagcc ttagcaaatt
      361 attggtatta caacagcaat attaggggcg tggtatacgc aggactgagt cgtgatatta
      421 gaaaagaact ggcctatgtg attaatggca ggttcctgag aaaagatatt aagaaagata
      481 aaatcacgga ccgggaaatg gaaattatcc gcatgacggc ccagggaatg caacctaaat
      541 cgattgccag aattgaaaat tgtagtgtga agacagtgta tacccatcgg cgtaatgctg
      601 aggccaagct gtactcaaaa atatataagt tggttcagta aactccaggc aagttagttt
      661 taaaaaatga ctcactggga catcacgtcc tcaattcaac tcgggaagaa atgcaatgaa
      721 aaaaaaggtt ctggcaatag ctctggtaac ggtgtttacc ggcatgggtg tggcgcaggc
      781 tgctgacgta acagctcagg ctgtagcgac ctggtcggca acagccaaaa aagacaccac
      841 cagtaagctg gttgtgacgc cactcggtag cctggcgttc cagtatgccg aaggcattaa
      901 aggttttaac tcacagaaag gtctatttga cgtggctatc gagggtgact caacggctac
      961 cgcctttaaa ctgacctcac gtcttatcac caacacatta acccagttgg atacctcagg
     1021 ttccacactg aatgtgggcg tggattataa cggcgcggca gtcgaaaaaa ctggcgatac
     1081 cgtgatgatc gataccgcca acggcgtact gggcggcaac cttagcccgc tggctaacgg
     1141 ttacaatgcc agcaatcgta ccaccgcaca ggatggtttc accttctcca tcatcagcgg
     1201 taccaccaat ggtaccaccg cagtaacaga ttacagcact ctaccggaag gcatctggag
     1261 cggcgacgtt agcgtacagt tcgacgcgac ctggaccagt taatctctct gatgtaccag
     1321 caggggtagc ccccctgctt tattcctgga cggaattgtt atgaaaaagc accttctgcc
     1381 tctcgctctg ctgttttccg gaatatctcc ggcccaggcg ctggatgtcg gcgatatatc
     1441 atcgtttatg aacagtgaca gcagcacgct gagcaaaacg atcaaaaaca gtaccgacag
     1501 tggtcgcctt atcaatatcc gtctcgaacg gctctcttca ccgcttgacg acgggcaggt
     1561 tatctcaatg gacaagccgg atgagttgct actcactccc gccagcttgc tgctacccgc
     1621 ccaagccagc gaagtgatcc gcttcttcta taagggaccc gcagatgaaa aagagcgcta
     1681 ctaccgcatt gtctggtttg atcaggccct cagtgatgcg cagcgcgata atgccaaccg
     1741 cagcgctgtg gccactgctt ccgcccgcat cggcaccatt ctggtcgtcg cccctcgcca
     1801 ggcgaactac cactttcagt acgccaacgg ctccctgaca aatacaggaa atgcgacgct
     1861 gcggatcctc gcctacggcc cttgcctgaa agccgccaac ggtaaggagt gtaaagagaa
     1921 ttactacctg atgccgggca agtcgcgtcg ttttacccgt gtggacactg cggataacaa
     1981 aggacgggtt gcactttggc agggtgataa gttcattccc gtgaaataga tagctgtgca
     2041 gatggataac gacaatgcct ttacgacggt tctccccagg actgaaagcc cagtttgcct
     2101 tcggcatggt ctttttgttc gttcaacccg atgccagcgc tgctgacata agtgcgcagc
     2161 aaataggtgg ggtgattatt ccgcaggcct tcagtcaggc gcttcaggac ggcatgagcg
     2221 tcccgctcta tattcatctc gccggtagcc agggtcgcca ggacgatcag cgaatcggca
     2281 gcgcttttat ctggctggat gatggacagc tacgcatccg gaaaatacag ctggaagaga
     2341 gtgaagataa cgccagtgtc agcgaacaaa ctcgacagca gctgatggct ctggccaacg
     2401 ccccgttcaa tgaggccctt accatccccc tgactgacaa cgcgcagctg gatctcagct
     2461 tgcgccaact gctgctgcag ctggtggtca agcgcgaagc gctgggcacc gtactacgct
     2521 cacgtagcga agacatcggg cagtccagtg ttaacaccct cagcagtaat ctgagctata
     2581 acttgggcgt ctataacaac cagttgcgta acggcgggag caacacatcc agctatctgt
     2641 cgctgaataa cgttactgcg ctgcgcgaac atcatgtggt gctcgacggc tcgctgtacg
     2701 ggatcggtag cggtcaacag gacagtgaat tatataaagc gatgtatgaa cgcgattttg
     2761 ccggtcaccg atttgccggt ggaatgctcg acacctggaa cttgcagtcc ttagggccga
     2821 tgaccgccat ttcagcaggg aagatttacg gcctttcctg gggaaaccag gccagctcca
     2881 ccatcttcga cagcagccag tcagccacgc cagtgatcgc ctttttaccg gcggcgggcg
     2941 aagtacatct cacccgtgat gggcggctac taagcgttca gaacttcact atgggcaatc
     3001 atgaagtgga tacccggggt ctaccgtacg ggatttacga tgtggaagtt gaggtgatcg
     3061 ttaacggtcg cgtgatcagc aaacgccccc agcgggtcaa taagctgttt agccgggggc
     3121 gcggcgtcgg tgcaccactg gcgtggcagg tatggggcgg tagctttcat atggatcgct
     3181 ggtcggaaaa cgggaaaaag acgcgaccag ctaaagagag ttggctagca ggtgcctcga
     3241 cctccggctc attgagtacg cttagctggg cggcaacggg atatggatac gataatcagg
     3301 cggtgggtga aacccgtctg acgctgccgc ttgggggggc gatcaacgtt aacctgcaaa
     3361 atatgctggc cagtgacagc tcatggagca gcatcggcag catcagcgcc actctaccgg
     3421 gaggctttag ttcgctgtgg gttaaccagg aaaaaacccg cattggcaat caattgcgac
     3481 gtagcgatgc cgacaaccgt gcaatcggcg gcacactcaa cctgaactca ctgtggtcga
     3541 agctgggcac attcagcatc agctacaatg atgaccgccg ttacaacagc cattattaca
     3601 cggcagatta ctatcaaaat gtctacagcg gtacctttgg ttcgcttggc ctgcgggccg
     3661 gtattcagcg ctataacaac ggcgacagca acgccaatac agggaaatat atcgctctcg
     3721 atctctcgct accactgggc aactggttta gcgcagggat gacccatcaa aacggctaca
     3781 ccatggcaaa cctgtcagca cgcaaacagt ttgatgaagg aaccattcgc actgttggtg
     3841 ccaatctgtc acgtgccatc tccggcgata ccggtgatga caaaaccctc agcggtgggg
     3901 cgtatgcaca gttcgacgct cgttacgcca gcggaacgct gaacgtcaat agcgcggcgg
     3961 acggctacat caataccaac ttgaccgcca atggcagcgt cggctggcag ggtaaaaaca
     4021 tcgctgccag cgggcggact gatggcaacg ctggggtgat attcaacacc gggctggagg
     4081 acgacggtca gatcagcgcc aaaatcaacg ggcggatttt cccgcttaac ggcaagcgta
     4141 actatctccc gctctctccc tatggaagat atgaggtgga gttacagaac agcaaaaact
     4201 cactcgacag ctacgatatc gtcagcggcc gcaaaagcca tctgactctc tatccaggca
     4261 atgtcgctgt cattgagcca gaggtgaagc agatggttac cgtctccggt cgtatccgtg
     4321 cggaagacgg cacactgctg gctaacgcac ggattaacaa ccatatcggc cgaacccgaa
     4381 ccgatgaaaa cggcgagttt gtcatggacg tggataagaa atatcccact atcgattttc
     4441 gctacagtgg caataaaacc tgcgaagtgg cactggaact caaccaggcg cgcggtgccg
     4501 tctgggtcgg tgatgtggtc tgcagcggcc tctcatcgtg ggcggcggtg acgcagacag
     4561 gagaagagaa tgagagttaa cctactaata acgatgataa ttttcgcgct aatctggcca
     4621 gtaactgagc tcagagcggc agtgagcaaa acaacctggg cggatgcacc ggcacgcgag
     4681 tttgtgtttg tcgaaaacaa ctcagacgac aactttttcg tcactcctgg cggggcgctg
     4741 gatccgcgcc tgaccggtgc caaccgctgg accggtttaa aatacactgg ttcaggaacc
     4801 atctatcagc aaagcctcgg ctacattgat aacggttaca acaccggcct ttataccaac
     4861 tggaagtttg atatgtggct ggaaaattca ccagtttcat ctcctttaac tggcttgcgc
     4921 tgcatcaact ggtacgctgg gtgtaatatg accaccagtc ttatcctgcc gcaaaccacc
     4981 gacgccagtg gattttatgg cgcgacggtg accagcggcg gcgcgaagtg gatgcacggc
     5041 atgttgtcag acgcgtttta ccagtatctg caacaaatgc ccgtcggcag cagctttaca
     5101 atgaccatca atgcctgcca gacctctgtg aactatgacg ccagcagcgg cgcacgctgt
     5161 aaggatcagg ccaccggcaa ctggtatgtt cgcaacgtca cccatacgaa agcagcaaat
     5221 ctgcggttaa taaataccca ctcgctggcg gaggtattta tcaacagcga cggagtaccg
     5281 actctgggcg aagggaacgc cgactgccgg acacagacca tcggcagcct ttcaggatta
     5341 agttgtaaga tggtcaacta taccctgcaa acaaacggac tcagcaacac ctcaatccat
     5401 atattcccgg cgatcgccaa ctcgtcgtta gcctcggccg tcggggcgta cgatatgcag
     5461 ttcagtctga atggcagttc atggaaaccg gtgagcaata ccgcctatta ctacaccttc
     5521 aacgagatga agagcgcaga ctcgatctat gttttcttct cgagcaactt ctttaagcag
     5581 atggtgaacc tcgggatcag cgatatcaac accaaagatc tattcaactt tcgctttcag
     5641 aacaccacat caccggagtc tggctggtat gaattttcta cctccaacac gctgattatc
     5701 aaaccccgtg atttcagcat cagtattatc tccgatgaat atactcagac accgtcgcgg
     5761 gagggatatg ttggcagcgg cgagtcggca ctcgatttcg gctatatcgt aaccaccagc
     5821 ggtaaaacag ctgccgacga agtgctgatc aaggtgaccg gacccgcgca ggtgattggc
     5881 gggcgctcct attgtgtctt cagctccgat gacggtaagg cgaaagtacc gttcccggcg
     5941 acactttcct ttattacccg caacggagct acaaaaacct acgatgccgg gtgcgatgat
     6001 agctggcggg atatgaccga tgcgctgtgg ttgaccacac cgtggactga tatctctggc
     6061 gaagtggggc agatggataa gaccacagtc aaattttcga ttccaatgga taacgccatt
     6121 tcgctgcgta cggtagatga taacggctgg tttggcgaag tcagcgcttc aggagagatt
     6181 catgttcagg cgacgtggcg taacattaac taaagccctg ctgacagcgg tctgtatgct
     6241 ggcggcacct ttgacacagg cgatttcggt cggcaatctg acattttcgc tgccgtccga
     6301 gactgacttt gtcagcaaac gtgtagtgaa taacaacaaa agcgcacgga tataccgtat
     6361 tgccatcagt gctattgata gcccgggcag cagtgaattg cgcacccgac cggtggatgg
     6421 tgaactgctt ttcgcccccc gccaactggc gttgcaggct ggtgagagcg agtattttaa
     6481 attttactat catggtccac gggataaccg cgagcgctac taccgggtct catttcgcga
     6541 agtccccact cgtaaccaga caagacgtag cccaaccggc ggcgaggtca gcacggagcc
     6601 ggtggtggtg atggatacca ttctggtagt acgaccacgt caggttcagt ttaaatggtc
     6661 cttcgacaag gtgacaggaa cggtaagtaa cactggcaac acatggttta agctactgat
     6721 taagccagga tgtgattcga ccgaagagga aggcgatgcc tggtatctac gtccgggaga
     6781 cgtggttcat cagcctgagt tacgtcagcc ggggaatcat tatctggtct ataacgacaa
     6841 attcattaag attagcgatt cctgtccggc taagccccct tcggcggact aagacttatc
     6901 catcggtcga gggaagaatt ccacctcaga gccccaaaaa tcgtttaaat gatgtggaag
     6961 cgatcgtcaa tatcacctgt aacgatgggc tttctcatat gcacaccata gtgttcagga
     7021 gacacggaaa ggtatcaggc agcctgctaa cgagtggttt aatcacgaat tagtacgtaa
     7081 aatcggtaac ggctggaaat cattcaatac tcacactatc gaaagttccc cagccaaccg
     7141 cggtacgttc ttacatacga tgtaccgctg ttctctttac gatttttagc tgtactggtg
     7201 aattatgagc aatctgaatc catgcatgac gtgtggtgcc tgttgtgcat ttttccgcgt
     7261 ctctttttac tgggccgaag ccgacgatgc tggcggtact attcccgcca ggctcactga
     7321 acaaatatcc ccttttcacc gatgcatgag cggtaccaat cagaaaaacc cccgatgtat
     7381 tgcccttgca ggaaccccgg gcaaaaatgc ctgctgcacg atatataaaa atcgatcgtc
     7441 cacatgcaga gaattcgcca tgtctggtga gaacggagtc gtcaatgagg cttgcaatcg
     7501 tgcaagggct aaatacgggc tgacaccgtt ataaacatac aataattaat tgcaatgccc
     7561 cgccagcgat aatagcgggg cttcgttttt caggggtaac aaaacccgat acttcttcta
     7621 tttgccagca acaatgcctc tcttctgtag cgttcttcgg atctacatca tcctgaagta
     7681 gcgcgatata ctcactgact atctgcatca gaatataaaa agcaatgttt ttaacctata
     7741 aaaatggcgc tgtatttgcg ccatttttat cattcaatgc attatctgtt tgagcctaaa
     7801 gggatctcag ggtctggctc atgagtaatt ctgtttcgta aatctctgcg aataatttca
     7861 atagaccaga accagactaa atgtccaaaa atttcagaaa cattctcata ccacgggaga
     7921 tcaaacagag gtggcgtcag tcccatgaga gggaatgaaa tcatatgaac aaaaagttgg
     7981 gctaaagcac ctgccagtaa gccctgccag agtttaattt ttggaaatac ttcagcgacc
     8041 acacaataac cgacagcaaa cactatcgaa aagataatgt gcgtaacacc aacccagtta
     8101 aagacatgcc cggcaaaggt ataaacagcc gcattgggat ctgtcagccc caaccagtct
     8161 cgaagaaaaa tatacggtgg attgagaaaa ttacgcgagc aatcaatttg gcctgcagcc
     8221 ctgattaatg attccgggcc acacgctgca ttaaacatat ccaccgggct acgtggcggc
     8281 aatggaactt cagcccccca cttcacgaat gcggaaacaa cgccagcaat cagcccaatg
     8341 aatgcagcaa gaccataacg tctgcggttc ggtggagttt gttcaaatat attcatatct
     8401 accctgcttg taccattatg ttatacacct cttcaggagt attcataaaa caaggcaaat
     8461 gtaaagaact gtattgtttt gtataacaag atggtttcct aatcgccaat gaatataagc
     8521 tccatcattt ctccctattt ttatattaga agtgacagag atttgcaggg tgatgcagag
     8581 ctgaaaatca caggtttcct tattggtttt tgcatcgtac aactaaagca ataaaccagc
     8641 tcccatcatc atcaacatcc cggcgaacat gaatttatta gccgggctca tagctgcatt
     8701 cgcgttttaa gaataatcct cctgctgtcg ccgactatgc ttaacattta aaaaagcatc
     8761 agcactctcg caacgcactc ttattttccc ctttagaata ccggaggcct ggtatgagca
     8821 accaaggcga ataccccgaa gataatcggg ttgggaagca cgagccgcac gatttaagtt
     8881 tgacccgtcg cgatctgatt aaagtgagcg ccgcaacagc ggcgaccgcc gtggtttatc
     8941 ctcattctac gctggcggca agcgttccgg cagctacacc cgcgccagag ataatgcccc
     9001 tgacactgaa ggtgaacggc aaaaccgagc agcttgaggt ggatacccga accacgctac
     9061 tggacgcttt gcgtgaaaat ctgcatttga tcggtaccaa gaaaggttgc gatcacggac
     9121 agtgcggagc ctgtaccgtg ctggtcaatg gtcgcaggct taatgcctgc ctgacgcttg
     9181 cagtcatgca tcagggggcc gagatcacca ccattgaagg cctgggttcg ccagataatc
     9241 ttcaccccat gcaggcggcc tttatcaagc atgatggctt ccagtgcggc tactgcacct
     9301 ccgggcaaat ttgctcatca gtagcggtgc taaaagagat tcaggacggc attcccagtc
     9361 acgtcacggt cgatttggtt tccgctccag aaacaactgc cgatgagatc cgtgaacgta
     9421 tgagcggcaa catctgtcgc tgtggtgcat acgctaacat ccttgccgcc attgaagatg
     9481 ctgcggggga gataaaatca tgaaggcgtt tacctatgaa cgagtgaaca ccccagccga
     9541 ggcggcactt agcgctcagc gcgtacccgg cgcaaaattt atcgcgggcg ggaccaatct
     9601 gctggacctg atgaagctgg aaattgaaac gcccacccac cttatcgatg tgaacggcct
     9661 cgggctcgat aagattgaag tgaccgacgc gggtgggctg cgcatcggcg cactggtacg
     9721 gaacaccgac ctggcggctc acgagcgcgt gcgtcgtgat tacgcggtac tctcccgcgc
     9781 cctgctcgct ggcgcatctg gtcagttacg taatcaggca accaccgcag gtaatctgct
     9841 ccagcgcacg cgctgcccct atttttacga caccaatcag ccctgcaata agcgcctgcc
     9901 cgggagcggc tgcgcggcgc ttgaaggctt tagccgtcag cacgcggtgg taggcgtaag
     9961 tgaagcctgc atagccaccc atccgagcga tatggcggtc gcaatgcggt tgctggatgc
    10021 ggtggtggaa accatcacgc cggagggaaa gactcgcagt atcacactgg ctgattttta
    10081 tcacccaccg gggaaaacgc cgcacattga aaccgccctg cttcccggtg agcttatcgt
    10141 tgcggtgacg ttacctccac cgctcggcgg aaaacatatc taccgtaagg tgcgcgatcg
    10201 cgcctcctac gcctttgccc tggtatcggt cgcggcgatt attcagcctg acggcagcgg
    10261 gcgcgtcgcg ctgggcggag tagcacataa gccctggcgc attgaggctg cggatgctca
    10321 gctatcccag ggggcgcagg ccgtatatga cacgctgttc gccagcgccc atcccaccgc
    10381 tgaaaacacc tttaaactcc tgttggcgaa gcgaacgctt gcctccgtac tggctgaagc
    10441 gagggcacag gcatgaaatt tgataaaccc gcaggggaaa acccgatcga tcagctgaag
    10501 gttgtcggtc gtccccatga ccgcatcgac ggaccgctga aaactaccgg cacggcacgc
    10561 tacgcctacg aatggcatga agaggccccc aacgccgcct atggctatat cgtcggttcc
    10621 gccattgcca aaggacgcct caccgccctt gatacggacg ccgcgcaaaa agctccgggc
    10681 gtactggctg tcattaccgc cagtaacgcc ggggcactcg gcaaaggcga caaaaacacc
    10741 gccaggctgt taggcggccc cactattgag cactatcatc aggccattgc gctggtagtg
    10801 gccgagacct tcgaacaggc gcgagcggcg gcctcgctgg tgcaggcaca ctatcgccgt
    10861 aataaaggag cttactccct ggcggacgaa aaacaggccg tcaatcagcc gccggaagac
    10921 acgcccgaca aaaacgtcgg tgactttgac ggggctttca cctccgctgc ggtgaagatt
    10981 gatgctacct acacgacccc ggaccagagc catatggcga tggagccgca tgcctcgatg
    11041 gccgtctggg atggaaataa gcttaccctc tggacctcaa atcagatgat tgactggtgc
    11101 cgcaccgatc tggcaaaaac gctgaaagtt cccgtggaga atgtgcgtat tatctccccg
    11161 tatatcggcg gagggtttgg cggcaagctg ttcctgagaa gcgatgcgct gctggcggcc
    11221 ctcgccgccc gagcggtgaa acgtccggtt aaagtgatgc tcccccgccc ctctattccc
    11281 aataacacca cgcaccgccc cgccaccctt cagcacttgc gtatcggtgc cgaccagagc
    11341 gggaaaatca ccgctatctc acatgaaagc tggtctggaa acctgcccgg cggcacgccg
    11401 gaaacggcgg tacagcaaag cgaattactc tacgccgggg cgaatcgtca taccggcctg
    11461 cggctcgcca cgcttgattt gccggaaggg aacgccatgc gtgcgcccgg cgaagccccc
    11521 ggtctgatgg cgctcgaaat cgcgatcgac gaactggcgg aaaaagcggg catcgatccc
    11581 gtcgagtttc gcatcctgaa tgacactcag gttgaccccg ccgacccgac gcgctgcttc
    11641 tctcgccgtc agcttatcga gtgcttgcgc accggagcgg ataaatttgg ctggaagcag
    11701 cgcaacgcca cacccggaca ggtgcgcgac ggggagtggc tagtcggcca cggtgttgcg
    11761 gcgggctttc gcaataatct gctggaaaaa tcgggtgctc gggttcacct cgaacaaaac
    11821 ggcaccgtta ccgtagaaac ggacatgacc gacattggca ccggcagcta caccattctg
    11881 gcccagacgg cagcggaaat gcttggcgta ccgctggagc aggttgcggt tcacctcggc
    11941 gattccagtt tcccggtttc tgcgggttct ggtggacaat ggggcgcgaa tacctccacc
    12001 tccggcgttt acgccgcctg tatgaagctt cgcgaaatga ttgcctcggc agtcgggttt
    12061 gatcctgagc agtcgcagtt tgccgacggc aagattacca acggtacccg aagcgccacg
    12121 ctacatgaag ccaccgcagg cggcagactg acagcggaag agagcattga attcggaaca
    12181 ctgagcaaag agtaccagca gtcgaccttt gccgggcatt ttgtggaggt cggcgtgcat
    12241 agcgcgacgg gagaagttcg ggtccggcgt atgctcgctg tgtgtgctgc aggacgcatc
    12301 ctgaatccga aaactgcgcg cagccaggtc attggcgcaa tgactatggg catgggcgcg
    12361 gcactgatgg aggagctggc ggtggatgac cgtttgggct acttcgttaa tcacgatatg
    12421 gcggggtatg aggtgccggt ccatgcggat atcccaaaac aggaggtgat tttcctggat
    12481 gataccgacc ccatatcctc cccgatgaag gccaaaggtg tcggtgagtt gggcctgtgc
    12541 ggcgtgagcg cggctatcgc caacgcggtg tataacgcca ccggtattcg ggtacgggat
    12601 tatcccatca ctctggataa gctgctcgat aagctgccgg atgtggttta aggagaaaca
    12661 atgtcatacc cgctttttga taaagacgaa cactggcata agccagagca ggcgtttctc
    12721 accgatgacc accggaccat tctgcgcttc gccgtagagg cgctaatgtc cggtaaagga
    12781 gcggtgctgg tgacgctggt ggagatacac ggcggcgcgg cgcgcccgct cggggcgcag
    12841 atggtggttc gcgaagatgg tcgttactgc ggttttgtct ctggcggctg cgtggaggcc
    12901 gctgccgctt ttgaagcgct ggagatgatg ggctcaggcc gcgatcgcga aattcgctat
    12961 ggcgaaggtt cgccgtggtt tgacatcgtt ctgccctgcg gcggtgggat cacgctgatg
    13021 ctccataaac tacgctcggc acagcctctg ctcgccgtgc tgaaccgtct ggaacagaga
    13081 aaaccggcgg ggctgcgcta cgatccgcaa gcacaatcgt tggtgtgcct gcccacgcaa
    13141 acccgaacgg gctggaatct caatggcttt gaggtggggt tcaggccatg cgtcaggctg
    13201 atgatttacg gacgttctct tgaggcgcag gcaaccgcga gtcttgcagc agccacaggc
    13261 tatgacagcc atatcttcga tctttttccg gcctcagcca gcgctcagat cgataccgac
    13321 acggcggtca ttttgctgtg ccatgatctc aaccgggagc tgccagcatt gcaggccgcg
    13381 cgagaagcaa aaccctttta tctcggcgca ttgggcagcc atcgaaccca cacgttacgt
    13441 ctgcaaaagc tccacgagct gggatggtcc agggaggaga cagcgcaaat ccgggccccc
    13501 gtcgggatat tccccaaagc ccgggatgcg catactctgg cactctccgt gctggcagaa
    13561 atcgcctctg tacgtctcca tcatgaggag gattcatgcc tgcccccgtc gtcctgatcc
    13621 ttgcggccgg gcgtggagag cgctttctcg cctccggggg aaatacccat aagtgtatcg
    13681 gctggcgtca gtccccggag gttgcgcctt atcgctggcc atttgaagaa aacgggagaa
    13741 ctttcgacct tgcgattgaa ccgcagatta cgactaatga tctgcgtctg atgttgaggc
    13801 tggctcttgc cggcggagga ataacaattg ccactcagga aactttcagg ccatatattg
    13861 aaagcggtaa gcttgtatcg ctgcttgatg actttcttcc acaatttccg ggcttctatc
    13921 tgtatttccc acagcgtcgc aatattgcac caaagctccg cgccctgatt gactacgtca
    13981 aagaatggcg gcagcaattg gtttaaatag ctgcacctgc actgcctgat gtcagaacag
    14041 tattttgatg aattgccaag gttacaatgg cacaaatgcg gcacaggagg aaacgtggtg
    14101 tatttaaata tggggtaact ctttgattt
//
LOCUS       DAPHDW010000119        14123 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.120, whole genome shotgun sequence.
ACCESSION   DAPHDW010000119 DAPHDW010000000
VERSION     DAPHDW010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14123
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.120"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            172..1230
                     /gene="citC"
                     /locus_tag="RH057_003430"
     CDS             172..1230
                     /gene="citC"
                     /locus_tag="RH057_003430"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HDV3129747.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAPPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKTPA"
     gene            1245..1541
                     /gene="citD"
                     /locus_tag="RH057_003431"
     CDS             1245..1541
                     /gene="citD"
                     /locus_tag="RH057_003431"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HDV3129748.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILRARLEALLARASGIPALPWEDCQ"
     gene            1538..2446
                     /gene="citE"
                     /locus_tag="RH057_003432"
     CDS             1538..2446
                     /gene="citE"
                     /locus_tag="RH057_003432"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HDV3129749.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHARRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2457..3989
                     /gene="citF"
                     /locus_tag="RH057_003433"
     CDS             2457..3989
                     /gene="citF"
                     /locus_tag="RH057_003433"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HDV3129750.1"
                     /translation="MTQKIEQSQRQERVAAWNRRAECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVDRVGDAAKIGAGATRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDTQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            3993..4544
                     /gene="citX"
                     /locus_tag="RH057_003434"
     CDS             3993..4544
                     /gene="citX"
                     /locus_tag="RH057_003434"
                     /EC_number="2.7.7.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
                     /protein_id="HDV3129751.1"
                     /translation="MHLLPELASHHAVSIPELLVSRDERQARQHVWLKRHPVPLVSFT
                     VVAPGPIKDSEVTRRIFNHGVTALRALAAKQGWQIQEQAALVSASGPEGMLSIAAPAR
                     DLKLATIELEHSHPLGRLWDIDVLTPEGEILSRRDYSLPPRRCLLCEQSAAVCARGKT
                     HQLTDLLNRMEALLNDVDACNVN"
     gene            4519..5397
                     /gene="citG"
                     /locus_tag="RH057_003435"
     CDS             4519..5397
                     /gene="citG"
                     /locus_tag="RH057_003435"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HDV3129752.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSQLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMAINGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPADLDYLRQFDRECIERNLSP
                     GGSADLLILTWFLAQI"
     gene            5448..6911
                     /gene="citT"
                     /locus_tag="RH057_003436"
     CDS             5448..6911
                     /gene="citT"
                     /locus_tag="RH057_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
                     /protein_id="HDV3129753.1"
                     /translation="MSLAKDNIWKLLAPLVVMGVMFLIPVPDGMPPQAWHYFAVFVAM
                     IVGMILEPIPATAISFIAVTICVIGSNYLLFDAKELADPAFNAQKQALKWGLAGFSST
                     TVWLVFGAFIFALGYEVSGLGRRIALFLVKFMGKRTLTLGYAIVIIDILLAPFTPSNT
                     ARTGGTVFPVIKNLPPLFKSFPNDPSARRIGGYLMWMMVISTSLSSSMFVTGAAPNVL
                     GLEFVSKIAGIQISWLQWFLCFLPVGVILLIIAPWLSYVLYKPEITHSEEVATWAGDE
                     LKTMGALTRREWTLIGLVLLSLGLWVFGSEVINATAVGLLAVSLMLALHVVPWKDITR
                     YNSAWNTLVNLATLVVMANGLTRSGFIDWFAGTMSTHLEGFSPNATVIVLVLVFYFAH
                     YLFASLSAHTATMLPVILAVGKGIPGVPMEQLCILLVLSIGIMGCLTPYATGPGVIIY
                     GCGYVKSKDYWRLGAIFGVIYISMLLLVGWPILAMWN"
     gene            7025..7831
                     /gene="rna"
                     /locus_tag="RH057_003437"
     CDS             7025..7831
                     /gene="rna"
                     /locus_tag="RH057_003437"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HDV3129754.1"
                     /translation="MKAFWRNAALLAVSLLPFSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKADFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKVGY"
     gene            8061..8471
                     /gene="rnk"
                     /locus_tag="RH057_003438"
     CDS             8061..8471
                     /gene="rnk"
                     /locus_tag="RH057_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HDV3129755.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8702..9940)
                     /locus_tag="RH057_003439"
     CDS             complement(8702..9940)
                     /locus_tag="RH057_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HDV3129756.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITATAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRMQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNAQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10161..10589
                     /gene="uspG"
                     /locus_tag="RH057_003440"
     CDS             10161..10589
                     /gene="uspG"
                     /locus_tag="RH057_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HDV3129757.1"
                     /translation="MYKTIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10710..12275)
                     /gene="ahpF"
                     /locus_tag="RH057_003441"
     CDS             complement(10710..12275)
                     /gene="ahpF"
                     /locus_tag="RH057_003441"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HDV3129758.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAEKRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGDIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12446..13009)
                     /gene="ahpC"
                     /locus_tag="RH057_003442"
     CDS             complement(12446..13009)
                     /gene="ahpC"
                     /locus_tag="RH057_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HDV3129759.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13381..>14123
                     /gene="dsbG"
                     /locus_tag="RH057_003443"
     CDS             13381..>14123
                     /gene="dsbG"
                     /locus_tag="RH057_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308670.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HDV3129760.1"
                     /translation="MLKKILLLALLPAIAFAEELPAPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFADPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQEYESSGGKLKLNVPANVSTEQMKVLSANEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGN"
BASE COUNT         3341 a         3743 c         3570 g         3469 t
ORIGIN      
        1 atcaaaattt cacgccgaaa tactccttag gatgtatagc gaaaggagaa aaagatatac
       61 ctcgatcacc cccctttctc ccaagtgaaa ataaaaggtt atcagtttgc aacattgaac
      121 aacattcgtt gcaaatcgat aacaacatgc accttcagga tactatttat tatgttcggc
      181 aatgatattt tcacccgcgt aaaacgttca gaaaataaaa aaatggcgga aatcgcccaa
      241 ttcctgcatg aaaatgattt gagcgttgac accacagtcg aagtatttat taccgtaacc
      301 cgcgatgaaa agcttatcgc gtgcggtgga attgccggaa atattattaa atgcgttgct
      361 atcagtgaat ccgttcgcgg tgaaggactg gcgctgacat tagccactga actgataaac
      421 ctcgcctatg agcggcacag cacgcatctg tttatttata ccaaaaccga atacgaggcg
      481 ctgttccgcc agtgcggttt ttccacgctg acctgcgtac ccggcgtgat ggtgctgatg
      541 gaaaacagcg ccacgcgact gaaacgctat gccgaatcgc tgaaaaaatt tcgtcatcca
      601 gggaacaaga ttggctgcat tgtgatgaac gccaatccct ttacgaatgg tcaccgttat
      661 ctgattcaac aagctgcggc acagtgcgac tggttgcatc tgtttttagt caaagaagat
      721 tcttcacgct tcccctatga agaccggctg gatctggtgt taaaaggcac cgccgatatt
      781 ccacgcctga ctgtgcatcg cggctccgaa tacatcatct cccgcgctac gttcccttgc
      841 tacttcatta aagaacagag cgtcattaac cattgttaca ccgaaattga tcttaagatt
      901 ttccgtcagt acctcgctcc cgcgctgggt gtaactcacc gctttgtcgg tactgaaccc
      961 ttttgtcgcg ttaccgccca gtacaaccag gatatgcgct actggctgga aacgccgact
     1021 atctccgcac cgcccatcga actggttgaa attgagcggc tgcgttacca ggagatgccg
     1081 atatccgctt cccgggtacg tcaactgctg gcgaaaaacg atctcacggc tatcgcgccg
     1141 ctggtccctg cagtcacgct gcattatttg cagaacctgc ttgagcactc ccgccaggac
     1201 gcggcagctc gtcaaaagac ccccgcatga gaaacaggtg aaaaatgaaa ataaaccagc
     1261 ccgccgttgc aggcaccctt gagtctgggg atgtgatgat acgcatcgcc ccactcgata
     1321 cgcaggatat cgacctgcaa atcaatagca gcgttgagaa acagtttggc gatgcaattc
     1381 gcaccaccat tctggacgtt ctcgcccgct acaacgtgcg cggcgtacag ctgaatgtcg
     1441 atgacaaagg cgcactggac tgcattttac gtgcacgact ggaagccctg ctggcacgcg
     1501 ccagcggtat cccggctctg ccatgggagg attgccaatg atttccgctt cgctgcaaca
     1561 acgtaaaact cgcacccgcc gcagcatgtt gtttgtgcct ggtgccaatg ccgcgatggt
     1621 cagcaactcc ttcatctacc cggctgatgc cctgatgttt gacctcgaag actccgtagc
     1681 attgcgtgaa aaagacaccg cccgccgcat ggtttaccac gcgctgcaac atccgctgta
     1741 tcgcgatatt gaaaccattg tgcgtgtcaa cgcgctggat tccgaatggg gtgtcaacga
     1801 cctggaagcc gtcgttcgcg gtggtgcgga cgttgtgcgt ctgccgaaaa ccgataccgc
     1861 tcaggatgtt ctggatatcg aaaaagagat cctgcgtatc gaaaaagcct gtggtcgtga
     1921 acccggcagc accggcctgc tggcggcgat tgaatctccg ctgggcatta cccgcgcagt
     1981 ggaaatcgct cacgcttccg agcgtttgat cggtatcgcc ctcggtgcag aagactatgt
     2041 gcgcaacctg cgtacagaac gctccccgga aggaactgaa ctgctgttcg cacgctgttc
     2101 cattttgcag gccgcgcgct ctgcgggtat tcaggcgttc gataccgtct attccgacgc
     2161 taacaacgaa gccggattcc tgcaagaagc cgcccacatc aaacagctgg gctttgacgg
     2221 caaatcgctg atcaacccgc gtcagattga tctgttgcac aacctctacg caccaaccca
     2281 gaaagaagtg gatcacgccc gccgcgtcgt agaagccgct gaagccgccg ctcgcgaagg
     2341 cctcggcgtg gtttccctga acggcaagat ggtggacggt ccggttatcg atcgcgcccg
     2401 tctggtgctc tctcgtgcag aactttccgg catccgcgaa gaataaggca atcaaaatga
     2461 cgcagaaaat tgaacaatct caacgacaag aacgggtagc ggcctggaat cgtcgcgctg
     2521 aatgcgatct tgccgctttc cagaactcgc caaagcaaac ctaccaggct gaaaaagcgc
     2581 gcgatcgcaa actgtgcgcc aacctggaag aagcgattcg tcgctctggt ttacaggacg
     2641 gcatgacggt ttccttccat cacgctttcc gtggcggtga cctgaccgtc aatatggtga
     2701 tggacgtcat cgcgaagatg ggctttaaaa acctgaccct ggcgtccagc tccctgagtg
     2761 attgccatgc gccgttggta gaacacattc gccagggcgt ggttacccgc atttatacct
     2821 ccggcctgcg cggtccactg gcggaagaga tctcccgtgg tctgctggca gaaccggtgc
     2881 aaatccactc tcacggcggt cgcgtgcatc tggtacagag cggcgaactg aatatcgacg
     2941 tggctttcct cggcgtcccg tcctgcgatg aattcggtaa tgccaacggc tacaccggta
     3001 aagcctgctg cggctccctc ggctatgcaa tggttgatgc cgacaacgca aagcaggtcg
     3061 tgatgcttac cgaagaactg ctgccttatc cgcataatcc ggcaagcatt gagcaagatc
     3121 aggttgattt gatcgtcaaa gttgaccgcg ttggcgatgc tgcaaaaatc ggcgctggcg
     3181 cgacccgtat gaccactaac ccgcgcgaac tgcttattgc ccgtagcgct gcggatgtga
     3241 ttgtcaactc aggctacttc aaagaaggtt tctccatgca aaccggcacc ggcggcgcat
     3301 cgctggcggt aacccgtttc ctggaagaca aaatgcgtag ccgcgatatt cgcgccgact
     3361 tcgcccttgg cggtattacc gcgacgatgg ttgacctgca cgaaaaaggt ctgatccgca
     3421 aactgctgga tgtgcagagc tttgacagcc atgctgcgca atcgctggcc cgtaacccca
     3481 atcacatcga aatcagcgcc aaccagtacg ctaactgggg ttcgaaaggc gcatcggttg
     3541 atcgtctcga cgtggtggta ctgagcgcgc tggaaattga cacccagttc aacgttaacg
     3601 tgctgaccgg ctctgacggc gtactgcgtg gtgcttccgg tggtcactgc gataccgcga
     3661 ttgcctctgc gctttccatc atcgtcgcgc cgctggtacg cggtcgtatt ccgactctgg
     3721 tggataacgt gctgacctgc atcaccccgg gctccagtgt cgatattctg gtcacagacc
     3781 acggtatcgc agttaacccg gcacgtccgg aactggcaga acgtctgcag gaagcgggca
     3841 ttaaagtggt ttccattgag tggctgcgcg aacgtgcgcg tctgctgacc ggtgaaccac
     3901 agccgattga attcaccgac cgcgtcgttg ccgttgtgcg ttaccgcgat ggctcggtga
     3961 tcgatgttgt gcatcaggtg aaggaataag ccatgcacct gcttcctgaa ctcgccagcc
     4021 accatgcggt gtcaattccc gagctgctcg tcagccggga tgaaaggcaa gcacggcaac
     4081 acgtctggct caagcgccat cctgttccac tggtctcctt taccgtggtt gcgcctgggc
     4141 cgattaaaga cagcgaggtc acacgccgaa tttttaatca tggcgtgaca gccttgcgtg
     4201 ccttagccgc aaaacagggc tggcaaattc aggagcaggc tgcactggtt tccgccagcg
     4261 ggccggaggg catgttgagc attgccgccc cggctcgcga cctcaagctc gccaccattg
     4321 agcttgaaca tagtcatcct ctcgggcggt tatgggatat cgatgtcctg acgcccgaag
     4381 gcgaaattct ctcccgccgc gactattcac tgccgcctcg ccgctgcctg ttgtgcgagc
     4441 aaagcgcagc cgtctgcgcg cgtggaaaaa cccatcaact gaccgattta ctcaaccgca
     4501 tggaggcact gctgaacgat gtcgatgcct gcaacgtcaa ctaaaaccac aaagcttgcg
     4561 acgtcattaa tcgatgagta cgccctgctg ggctggcgcg ccatgctgac tgaagtcaat
     4621 ctgtcaccga aaccaggcct cgtggatcgc attaactgcg gtgcgcacaa agatatggcg
     4681 ctggaagatt tccaccgcag cgcgctggcg attcagggct ggttaccccg tttcattgaa
     4741 tttggtgcct gtagtgcaga aatggcaccg gaagcggtac tccacggatt acgcccaatt
     4801 ggtatggctt gcgaaggtga tatgttccgc gccactgcgg gcgtaaacac gcataaaggc
     4861 agcatttttt ctttagggct gctatgtgcg gcaattggcc gtttgcttca gctcaaccag
     4921 ccggtaacgc caacaaccgt ttgttctacg gcggcaagtt tctgccgtgg tctgaccgat
     4981 cgcgaactgc gtaccaataa ttcacaactg acggcaggtc aacggttgta ccagcagctt
     5041 ggccttaccg gcgcacgcgg tgaagccgaa gcgggttatc cactggtgat caaccacgcc
     5101 ttgccgcatt acctcactct gctggatcag gggttagatc ctgaactggc attgctcgat
     5161 accttgctcc tactgatggc gatcaacggc gataccaacg ttgcttcgcg cggtggcgag
     5221 gggggcctgc gctggctaca gcgcgaggcg caaacattat tgcaaaaagg gggcattcga
     5281 acccccgccg atctcgatta tctccggcag ttcgacaggg agtgtatcga acgaaatctc
     5341 agtccaggcg gcagtgccga cctgctgatc cttacctggt ttttagcaca gatttaatta
     5401 tttaagcact tgataaattt gaaaatatta attttcggag aacccgtatg tctttagcaa
     5461 aagataatat atggaaacta ttggccccac tggtggtgat gggtgtcatg tttcttatcc
     5521 ctgtccccga cggtatgcca ccacaggcat ggcattactt cgctgtgttt gtggcaatga
     5581 ttgtcggcat gatcctcgag ccaattccgg caacagcgat cagttttatt gcggttacta
     5641 tttgcgttat tggcagtaat tacctgctct ttgatgccaa agaattagct gacccagcgt
     5701 ttaatgcgca aaaacaggcg ctgaaatggg gtctggctgg tttttccagc accacggtat
     5761 ggctggtatt tggcgcattt atttttgcat tagggtatga agtttccggg ttaggtcgtc
     5821 gcattgccct tttcctggtg aaattcatgg gcaaacgcac gctgacgttg ggttatgcga
     5881 ttgtcattat cgacattctg ctggcaccgt ttacaccgtc caacaccgcg cgtaccgggg
     5941 gtacggtttt tccggtcatt aaaaacctgc cgccgttgtt taaatcattc cccaacgatc
     6001 cgtccgcgcg tcgtattggc ggctatttga tgtggatgat ggtcattagt accagtctga
     6061 gttcgtccat gtttgtcacc ggtgcggcac caaacgtgct gggtctggag ttcgtcagca
     6121 aaattgccgg tatccagatt agctggttgc agtggttcct ctgcttcctg ccggttgggg
     6181 ttatcttgct tatcattgcg ccgtggcttt cctacgtgct gtacaaaccg gaaatcacac
     6241 acagtgaaga agtggcaacc tgggcgggtg atgaactaaa aaccatgggt gcgctgacac
     6301 gcagagagtg gacgctgatt ggccttgtat tgctcagctt aggtttgtgg gtatttggca
     6361 gtgaagtcat taatgctact gcggttggtc tgctggcagt ttcgctaatg ctggccctgc
     6421 atgttgtgcc gtggaaagac attacccgct ataacagcgc atggaacacg ctggtcaacc
     6481 tggcaactct ggttgtgatg gctaacggcc tgactcgttc tggttttatt gactggttcg
     6541 ccggtaccat gagtacacac ctggaaggat tctcaccaaa cgcaacggtg attgtactgg
     6601 ttctggtgtt ctactttgca cactacctgt ttgccagcct gtctgcgcac accgcaacca
     6661 tgctgccggt tattctggcc gtcggtaaag gtattccggg cgtaccaatg gaacaactgt
     6721 gtatcctgct ggtgctgtct atcggtatca tgggctgtct gacgccgtat gcaaccggtc
     6781 ctggggtgat tatttacggc tgtggctatg tgaaatcaaa agattactgg cgtcttggcg
     6841 caatcttcgg ggtgatttat atctctatgt tgctgttggt tggctggccg attctcgcca
     6901 tgtggaacta atacgttcgc caaacgctga ataaatcatc acgcccgcca ggttagccca
     6961 cctggcgggc ttttttatga tttaatagat agtattcagt gtatgtatga gttccacacc
     7021 cattatgaaa gcattctggc gtaacgccgc gttgctcgcg gtttctctgc ttcccttctc
     7081 ttctgccaac gccgtagcgt tgcaggcaaa acagtatggc gactttgatc gctatgtcct
     7141 ggccctctcc tggcaaaccg gattttgcca gagtcaacac gatcgaaatc gtaacgaacg
     7201 agatgaatgt cgcctgcaaa ccgaaacgac caacaaagct gattttctga ccgtacatgg
     7261 tctgtggcca ggattgccta aatcggttgc tgcccgtggt gttgatgaac gccgctggat
     7321 gcgcttcggt tgcgctactc gcccaatccc gaatctacca gaagcgcgcg ccagccggat
     7381 gtgttcatcg ccggaaaccg gattatcact ggaaacggcc gctaaactaa gtgaagtcat
     7441 gccaggagct ggcggacgtt cctgcctgga acgctacgaa tatgccaaac acggtgcctg
     7501 ctttggtttt gatccggacg catacttcgg tacgatggta cgcctgaatc aagaaattaa
     7561 agagagcgaa gccggaaaat tccttgcgga taattacggt aaaacagtga gccgccgtga
     7621 ctttgacgcc gcctttgcca aaagctgggg aaaagagaac gtgaaagcag ttaagctaac
     7681 gtgccagggt aaccctgcgt atttgactga aattcagatc tcgatcaaag ctgacgccat
     7741 caacgctccg ctttctgcaa actcattctt gccacaacca cacccaggca actgtggcaa
     7801 aacctttgtg attgataaag tgggttatta atcatctgat ttacaaaaca cgctaattcg
     7861 tcttccataa accgtgacga ttaagaatta tacataatgc gctgcattga ggaattccct
     7921 gaatgtgacg caaatcactt caaagagtga gcaacagtca gtatcatcat gtgagttaaa
     7981 ccctcgccgc ctgacggtga gggttttctt ttgggtatgt ttatctgcga cactcgcagt
     8041 accgacgaca tggagtaaaa atgtccagac caactatcat cattaacgac ctggatgccg
     8101 aacgcatcga tattctgctg gagcaacccg cctatgctgg tttgccaatc gccgacgcgt
     8161 taaacgcaga gttggatcgc gcccaaatgt gttcgccaga agagatgcca cacgacgtgg
     8221 tgacaatgaa cagccgggtt aaattccgca atcttagcga tggcgaagtg cgtgtgcgca
     8281 cgctggtgta tccggcaaaa atgaccgata gcaatactca gctttccgtt atggctccgg
     8341 taggtgccgc actgctgggg ctgcgcgttg gcgattccat tcactgggaa cttccgggcg
     8401 gcgttgcaac ccaccttgaa gtgctggaac tcgaatacca gccagaagct gctggcgact
     8461 acctgcttta atcccatctg cacaagcgat aactcgcttt gcccagagga tgcactcgca
     8521 tcctctttac gcaaaatcag caatttttcc tggttctcac tgttcacttt tgctgcaaaa
     8581 ttattccgtt tcgtttgtgg gaataaccat ggcagaagca ttttacatcc tgataggatt
     8641 tctgattatg gcggcgatta tcgtcatggc tgtcctttac ctcgaaaacc actcctgacg
     8701 atcatattgt tccccccggc atcgcattca ccagacctga aaccgccttc tgcgccgcct
     8761 ctgcgctttg tgcaccgggt accagaatca ccttacggca ctcctcttca cgtttttcga
     8821 aaatctcata tccccgggcg gcctcttcaa acggcatata gtgggtaaca atttcttctg
     8881 gtttcagtaa tcctttctca attaacggta ataattctcc cagccatgcg tgaacgtggg
     8941 tctgtcccat tttaaacgtc aaccctttat caaaggcgtc gccaaacagg aaaccgtgaa
     9001 taaatccagc gtagacgccc ggtacgctaa caatgccgcc acgcctgacc gccgcaatac
     9061 actggcgcaa cgctttaccg ctgctgccct ccagtttcag gttagtcagc accgtttccg
     9121 tggtgctgcc tttcgcttca aaaccgacgg cgtctattac tgcatccacg ccccggtgac
     9181 ctgccgtttg ttcaataatt gactgtgccg gatcgctatc ttcatcgaaa ttaatcggga
     9241 tcgcgccgta gcggtcagcg gcgaaacgca agcggtaggg atgatgatca acaacaaaaa
     9301 tctgttccgc accgagcaac cgtgcacagg cgattgtcaa caatcccact ggaccggcac
     9361 catagactgc aacgcttgaa ccttgttgga tctgcgcatt ttttgctgcc tgccatgccg
     9421 ttggcagaat atcagaaagg aaaagggctt tatcatctga aagcaaaggc ggtactttaa
     9481 acggccccac attcccttta gggacgcgga catattccgc ctgtccacca ggaacgccac
     9541 catacaggtg actataacca aacaatgccg cgggagctgg tatctgtttt ttattgagcg
     9601 cagcgccttt acccgcattg gtattttcgc aggcggcata ttgctgcatt cgacagaaaa
     9661 aacagtcgcc acaagcaatg acaaacggaa ttaccacccg gtcgcctttt tgcaaatttt
     9721 ttacgtcctt tccggtttca acgacttccc ccataaattc atgaccaaaa atatcgccat
     9781 gtttaacctg gggtattttg cctcgataaa gatggaggtc agagccacag atcgccgttg
     9841 ccgtaatacg cagaataata tcatctgcct gttcaatgcc cggatcggga acattttcta
     9901 cctgaacgtg atgtgggccg tgataagtca atgctttcat gcaacctcca gagacagttc
     9961 aaaaaagagt cagtctttaa gggtagctat cacagcggga aaacgggcgg taccgcaggt
    10021 tttgggagaa ttcctggtgt tcagtgagaa tatattaatc acagaaaccg ttcgtaagga
    10081 aaaagaacca gacaaatatc agggataaat attataatca tggcgaatga tttgttcatg
    10141 attaacaggg agaaagggtt atgtataaga caatcattat gccagttgat gtatttgaaa
    10201 tggaattgag cgacaaagct gttcgccacg ctgaattcct cgcccaggat gacggagtta
    10261 ttcatctact tcacgtacta cccgggtcag ccagcctgag cctgcaccgt tttgccgctg
    10321 atgtgcgtcg ttttgaagag catctgcaac atgaagcaga agaacgtctg caaacgatgg
    10381 tcagccactt caccatcgat ccttcccgca ttaaacaaca tgtccgtttt ggtagcgtgc
    10441 gggatgaagt caatgagttg gcagaagaac tgggggctga tgttgtagtt attggttctc
    10501 gcaacccatc gatttcgacc catctgttag gttctaacgc ctcgagcgtg atccgccatg
    10561 ccaatctgcc ggtgctggtt gtgcgttaat tgccagaata aatatcccgc cctgcccgga
    10621 gccatcccgg cagggctttt taatgccata aaaaagccgc caggtttgac cccggcggct
    10681 aagcaattgc aggtgaatct tacttcttct tatgcagttt tggtgcgaat caggtagtca
    10741 aaagcactca gagaggcttt ggcaccttcg ccagtggcga tgatgatctg cttgtacgga
    10801 accgtcgtac agtcacccgc tgcgaatacg cctttgacgt tggtttcgca tttcgcatca
    10861 atgataatct cacccatgcg gttacgttcg actgcgcctt ccagccagtt ggtgttcggc
    10921 agcagaccaa tctggacgaa aataccggcc agttcgatgt tgtgaatatc gccgctgaca
    10981 cgatctcgat attccagacc aacgactttg ctgccgtcgc ctttcacttc cgtggtttgc
    11041 gcattcagaa taatgtcgac gtttttcagg ctgcgcagtt tgtcctgcag aacctggtcg
    11101 gctttcattt ctggcgcaaa ttcgagcagc gttacgtgct caacgatacc cgccaggtca
    11161 attgccgctt ccacgccgga gttaccgccg ccgataaccg ctacgcgttt gcctttaaac
    11221 agcgggccgt cgcagtgcgg gcagtaggtc acgcctttgg tgcgatactg atcttcgcca
    11281 ggaacgttca tgttgcgcca ttttgcaccg gtcgccacga taatgctgcg tgctttcagt
    11341 accgcgccag aagctgtttc aatctgatgc agaccacctt caactgctgc tgggatcagt
    11401 ttgctggcgc tctggctgtc gatcacatca acgtcgtatt catcaacgtg aactttcagt
    11461 gcgcctgcca gcttctgccc ttcagtcttc ggtacagaaa tgtagttttc gatatcaacg
    11521 gtatcgagga tctgaccacc aaaacgttcg cccatcagac cggtacggat gcctttacgt
    11581 gcggagtaaa ttgctgccgc tgcacccgcc gggccggaac cgacgattaa tacgtcataa
    11641 gcatcacgct tgttcagctc ttctgccgca cgtttttccg cgccagtatc aattttggca
    11701 acgatttcag tcaacgtcat gcggccctga ccaaactctt tcccgtttac gaacactgcc
    11761 ggaacgccca tcacgttgcg atcggtgatt tcgttctgga aggtgccgcc gtcaattgca
    11821 gtgtgcttga tgcgcgggtt cagtacgctc atcaggttca gcgcctgcac cacgtccggg
    11881 cagttgtggc aagagagcga gtaataggtt tcgaattcaa aatcaccgtc aatatggcga
    11941 atctgctcca gcagagactg cgcttctttc gacggatgac caccggtcca cagcaacgcc
    12001 agtaccagcg aggtgaactc gtggcccagc ggggagcctg caaaacgtgg cccctggttg
    12061 gaacctgggt tggtgatcag gaaagacggc ttacgcaccg gcaagctgtt atcttcttta
    12121 aaggtgactt tgtctgacag ttctgcgatt tcagccaaca gttccttgat ttctgccgat
    12181 ttagcgctgt catccagcgt ggcaattaac tcaacaggct tggtcaattt ctcaaggtaa
    12241 gccttgagtt gagttttcat atttgtgtcg agcatgttta tctcctgggc tttaaacatc
    12301 atcatgcaag cggcctctgc ggctacctgg atgcagcttg catcatggtg cggggaagaa
    12361 aatcgggtgc cgcagcaccc gaagaattag gtgaatttcc gaagtatttc gcgccggagt
    12421 tggtagcgcg aaagacgaag gaaatttaga ttttaccaac caggtccaga gacggagcca
    12481 gagttgcttc accttctttc catttagccg ggcaaacttc acctgggtga gaagctacgt
    12541 actgtgctgc tttgatttta cgcagcaggt cagacgcgtc acggccaatg ccttcagcgg
    12601 taacttcgat tgcctggatg ataccctgcg ggtcaacaac gaaggtcgca cggtcagcca
    12661 gaccttcatc ttcacgcatg ttgtcgaagt tacgggtcag ggcgccagtc gggtcgccga
    12721 tcatcgcata tttgatttta gcgatggttt cagagctgct gtgccatgct ttgtgggtga
    12781 agtgagtatc ggtagatact gcgtatacgt ctacgcccag tttctgcagt tcttcgtagt
    12841 ggtcagcaac gtcacccagt tcggtcgggc atacgaaagt aaagtcagcc gggtagaaga
    12901 agaagacgct ccagcggcct tcggtatctt tttcggtgat ttcgatgaat tcgccgtttt
    12961 tgaatgcctg gtttttaaaa ggtttaattt tggtgttaat caaggacatc tatacttcct
    13021 ccgtgttttc gatgagatgt aaggtaacca atttttgccg attcggctaa tgcgtttcca
    13081 ttatcaaatc gataagtttt accttacaac cttcgtaaga caactttgtg aactttgcac
    13141 cgccagcggc gataaaaagt aaaatggccc cctttccagg tgaccgtgta atctgaacta
    13201 ccctttggta tcttcagtgc ctgcctgcag gctgaggtgt tgcccttaca aatgcaacaa
    13261 cgacatggat tacaacaccc tcataaacaa agggcaatca cctgatctaa gctcttacct
    13321 atgacagtga taggttatgc cttttactcg acttttgcac tgactgaaaa ggacaaatta
    13381 atgttaaaaa agatactttt actggctctg cttcctgcaa tcgccttcgc agaggaactt
    13441 cctgctccag taaaagcgat tgaaaaacag ggcattacaa tcatcaaaac attcgatgcc
    13501 cccggaggaa tgaaaggtta tctcggaaag tatcaggata tgggcgtcac catctacctg
    13561 actccagatg gtaagcacgc tatctctggt tacatgtaca acgagaaagg tgaaaacctg
    13621 agtaacacac ttatcgaaaa agaaatttac gcaccagccg gacgcgaaat gtggcaacgg
    13681 atggaacaat cccactggct cctcgacggt aaaaaagatg cgccggtcat tgtctacgtg
    13741 ttcgccgatc cgttctgccc atattgtaaa cagttctggc agcaggcgcg cccgtgggta
    13801 gattctggca aagtgcaatt aagaacattg ttggttgggg ttatcaagcc agaaagcccg
    13861 gcgacagcag cggcaattct tgcctccaaa gatcccgcaa aaacctggca agaatatgaa
    13921 tcctctggtg gcaagcttaa gctaaacgtg cctgcaaacg taagtacaga acaaatgaaa
    13981 gtgttaagcg ccaacgagaa actcatggac gatctggggg caaatgtcac accagcgatc
    14041 tattacatga gtaaggaaaa tacgctgcaa caggccgtgg ggttgcccga tcagaaaacg
    14101 cttaatatca ttatggggaa taa
//
LOCUS       DAPHDW010000120        13873 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.122, whole genome shotgun sequence.
ACCESSION   DAPHDW010000120 DAPHDW010000000
VERSION     DAPHDW010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.122"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(127..873)
                     /gene="cutC"
                     /locus_tag="RH057_003444"
     CDS             complement(127..873)
                     /gene="cutC"
                     /locus_tag="RH057_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HDV3129761.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDSLQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(887..1453)
                     /locus_tag="RH057_003445"
     CDS             complement(887..1453)
                     /locus_tag="RH057_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HDV3129762.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLNITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSENMINGRPICLFKLHEPVQVAHWQFSIVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            1669..3402
                     /gene="argS"
                     /locus_tag="RH057_003446"
     CDS             1669..3402
                     /gene="argS"
                     /locus_tag="RH057_003446"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HDV3129763.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQVRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDAAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDEFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            3579..4067
                     /locus_tag="RH057_003447"
     CDS             3579..4067
                     /locus_tag="RH057_003447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HDV3129764.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDISAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGVKKPDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYHNSEDFYPSPLT
                     RG"
     gene            complement(4187..4579)
                     /gene="flhe"
                     /locus_tag="RH057_003448"
     CDS             complement(4187..4579)
                     /gene="flhe"
                     /locus_tag="RH057_003448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HDV3129765.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAPLS
                     TRQPASGLMTLVAWRYQLIGPTPSGLRVRLCSQSRCVELEGQSGTTVAFSGIAAAEPL
                     RFIWEVPGGGRLIPPLKVQRNEVIVNYR"
     gene            complement(4579..6657)
                     /gene="flhA"
                     /locus_tag="RH057_003449"
     CDS             complement(4579..6657)
                     /gene="flhA"
                     /locus_tag="RH057_003449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HDV3129766.1"
                     /translation="MSNLAAMLRLPANLKSTQWQILAGPILILLILSMMVLPLPAFIL
                     DLLFTFNIALSIMVLLVAMFTQRTLEFAAFPTILLFTTLLRLALNVASTRIILMEGHT
                     GAAAAGKVVEAFGHFLVGGNFAIGIVVFVILVIINFMVITKGAGRIAEVGARFVLDGM
                     PGKQMAIDADLNAGLIGEDEAKKRRSEVTQEADFYGSMDGASKFVRGDAIAGILIMVI
                     NVVGGLLVGVLQHGMSMGHAAESYTLLTIGDGLVAQIPALVISTAAGVIVTRVSTDQD
                     VGEQMVNQLFSNPSVMLLSAAVLGLLGLVPGMPNLVFLLFTAGLLGLAWWIRGREQKA
                     PAEPKPVKMAENNTVVEATWNDVQLEDSLGMEVGYRLIPMVDFQQDGELLGRIRSIRK
                     KFAQEMGFLPPVVHIRDNMDLQPARYRILMKGVEIGSGDAYPGRWLAINPGTAAGTLP
                     GEATVDPAFGLNAIWIESALKEQAQIQGYTVVEASTVVATHLNHLISQHAAELFGRQE
                     AQQLLDRVAQEMPKLTEDLVPGVVTLTTLHKVLQNLLDEKVPIRDMRTILETLAEHAP
                     IQSDPHELTAVVRVALGRAITQQWFPGKDEVHVIGLDTPLERLLLQALQGGGGLEPGL
                     ADRLLAQTQEALSRQEMLGAPPVLLVNHALRPLLSRFLRRSLPQLVVLSNLELSDNRH
                     IRMTATIGGK"
     gene            complement(6650..7798)
                     /gene="flhB"
                     /locus_tag="RH057_003450"
     CDS             complement(6650..7798)
                     /gene="flhB"
                     /locus_tag="RH057_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HDV3129767.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIINDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRMFSAQTGAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRIRQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYDENKMSAPKVVAKGAGLVALRIREIGAENNVPTLEAPPLARALYRHAEIGQQIPGQ
                     LYAAVAEVLAWVWQLKRWRLAGGQRPVQPTHLPVPEALDFINEKPTHE"
     gene            complement(8000..8644)
                     /gene="cheZ"
                     /locus_tag="RH057_003451"
     CDS             complement(8000..8644)
                     /gene="cheZ"
                     /locus_tag="RH057_003451"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HDV3129768.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTRMLRDSLRELGLDQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(8655..9044)
                     /gene="cheY"
                     /locus_tag="RH057_003452"
     CDS             complement(8655..9044)
                     /gene="cheY"
                     /locus_tag="RH057_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HDV3129769.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(9059..10108)
                     /gene="cheB"
                     /locus_tag="RH057_003453"
     CDS             complement(9059..10108)
                     /gene="cheB"
                     /locus_tag="RH057_003453"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HDV3129770.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELSRSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLAKISAGQAIRI"
     gene            complement(10111..10971)
                     /gene="cheR"
                     /locus_tag="RH057_003454"
     CDS             complement(10111..10971)
                     /gene="cheR"
                     /locus_tag="RH057_003454"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HDV3129771.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEYRVWSAAASTGEEPYSIAMTLADTLGTAPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQNTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(10990..12591)
                     /gene="tap"
                     /locus_tag="RH057_003455"
     CDS             complement(10990..12591)
                     /gene="tap"
                     /locus_tag="RH057_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HDV3129772.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNQVE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQMEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSQEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMIDIVSSVTRVND
                     IMGEIASASEEQQRGIEQVAQAVSQMDQVTQQNASLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(12637..>13873)
                     /locus_tag="RH057_003456"
     CDS             complement(12637..>13873)
                     /locus_tag="RH057_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HDV3129773.1"
                     /translation="YGNTGAYFAQPTQGMQNAMGEAFAQYALSSEKLYRDIVTDNADD
                     YRFAQWQLAVIALVVVLILLVAWYGIRRMLLTPLAKIIAHIREIAGGNLANTLTIDGR
                     SEMGDLAQSVSHMQRSLTDTVTHVREGSDAIYAGTREIAAGNTDLSSRTEQQASALEE
                     TAASMEQLTATVKQNADNARQASQLAQSASDTAQHGGKVVDGVVKTMHEIADSSKKIA
                     DIISVIDGIAFQTNILALNAAVEAARAGEQGRGFAVVAGEVRNLASRSAQAAKEIKAL
                     IEDSVSRVDTGSVLVESAGETMNNIVNAVTRVTDIMGEIASASDEQSRGIDQVALAVS
                     EMDRVTQQNASLVQESAAAAAALEEQASRLTQAVSAFRLAASPLTNKPQTPSRPASEQ
                     PPAQPRLRIAEQDPNWETF"
BASE COUNT         3322 a         3885 c         3411 g         3255 t
ORIGIN      
        1 gctcaacaga tcacggttat cgttcgtttt ttatactgct cagggataaa aaaaggcccc
       61 tgttgaaatt gcaggggcct ggtacgagca agcatcatat tgggcgacat gatgcaacgg
      121 taaaaatcat ttggcctgat ggcgttcaat gattcctttc atttcagcaa ccgccgcccc
      181 gtctacgata taacgcgaat actcgtccgc gtgttcatct gatgacatgg acaatccttg
      241 attacgataa cgcatcggtg aggcttgcca cgctcccgcg gagctatgga cttccagcac
      301 tccggcatcg aggaagtggt gcaggttttc tgcacggact cctgctccgg ccataatgat
      361 tggagcatca cgatgggcaa taagttccat aatttttgat aaaccttgca gtgagtctga
      421 tttttgccct gatgtcagta cccgggcaat gcccaattcc gcaagattat tgagtgtata
      481 taaagggtta gcgcacatat cgaaggcgcg atgaaaagtc actgccagcg gaccggcagc
      541 agccattatt ttttccattc gtggcatatc gacattcccg tcaacatcga gaacgcccgt
      601 caccagtcca ggaaaaccta attcgcggac cgtgcgcaca tcctcaagaa tggcggcaaa
      661 ctcaccgtcg ctgtagcaaa aatcaccacc gcgtgggcga attatcggat gcacagggat
      721 cgtcacccgc tggcgcacgg atttcagtac ccccaacgac ggcgttaagc ccccctcttt
      781 tggggctgcg cataattcaa ctctgtctgc gccgttttgc tgcgccgtta gtgcacattc
      841 catgctgtaa cagcaaattt ccagtaatgc catttttgct ccttaattac gctgactgct
      901 cgctggcaac gatctcttca atggaccacg gatgaaactt aatagttgtt ttgccatcgg
      961 tgaccgccag cgtcggattc ggtaatcgct catgctcacc ttttggtgaa ctggttttaa
     1021 ctgagatccc cggtaggctg agtccttcat cagaaagcaa agccagcgca cgggcattca
     1081 gcgtttctgg atcacccggc agaacgattt caatatgttc ccaaccttcg tgtgggtaac
     1141 gtttttcccc gggccacggt agctccacaa tagaaaactg ccagtgcgca acctgtaccg
     1201 gttcatgcaa tttaaacaga caaatcggtc tgccattgat catattttct gacaaaagct
     1261 cgccacactg ttcaaacccg cgacgccagc gctcagcggt gacgttttga tggcaacgca
     1321 aagaaatgtg atcggcagtc agcggagtga tattcaaccc cagacggcgg gaaagttcat
     1381 ctaatgcgtg ggtaaatcgc ggtaaatccg atgcaatatc ctgcagctca tcgatagatt
     1441 gccagttcgc cataatcact cttcgtcttt cagtaaaagc gttaatttac cctgttgccc
     1501 tgtgtcaacc aaccgctgat ttcacgccgc ttctgatgca atagtgaaaa cggcaatacg
     1561 ccacgcgcac gttgctgacg aaaacagcca tttgcagtat actcccgccc taatttcttt
     1621 aactggtgcg ggcaattttt gctcgcttca tcaatgtaag gtattccggt gaatattcag
     1681 gctcttctct cagaaaaagt ccgtcaggcc atgattgcgg caggcgcgcc tgcggattgc
     1741 gaaccgcagg ttcgtcagtc agcaaaagtt cagttcggcg actatcaggc taacggcatg
     1801 atggcagttg ctaaaaaact gggtatggca ccgcgacaat tagcagagca ggtgctgact
     1861 catctggatc ttaacggtat cgccagcaaa gttgagatcg ccggtccagg ctttatcaac
     1921 attttccttg atccggcatt cctggctgaa catgttcagc aggcgctggc gtccgatcgt
     1981 ctcggtgttg ctacgccaga aaaacagacc attgtggttg actactctgc gccaaacgtg
     2041 gcgaaagaga tgcacgtcgg tcacctgcgc tctaccatta ttggtgacgc agcagtgcgt
     2101 actctggagt tcctcggtca caaagtgatt cgcgcaaacc acgtcggcga ctggggcact
     2161 cagttcggta tgctgattgc atggctggaa aagcagcagc aggaaaacgc cggtgaaatg
     2221 gagctggctg accttgaagg tttctaccgc gatgcgaaaa agcattacga cgaagatgaa
     2281 gagtttgccg agcgtgcacg taactacgtg gtaaaactgc aaagcggtga cgaatatttc
     2341 cgcgagatgt ggcgcaaact ggtcgacatc accatgacgc agaaccagat cacctacgat
     2401 cgtctcaacg tgacgctgac ccgtgatgac gtgatgggcg aaagcctcta caacccgatg
     2461 ctgccaggaa ttgtggcgga tctcaaagcc aaaggtctgg cagtagaaag cgaaggggcg
     2521 accgtcgtat tccttgatga gtttaaaaac aaggaaggcg aaccgatggg cgtgatcatt
     2581 cagaagaaag atggcggcta tctctacacc accactgata tcgcctgtgc gaaatatcgt
     2641 tatgaaacac tgcatgccga tcgcgtgcta tattacatcg actcccgtca gcatcaacac
     2701 ctgatgcagg catgggcgat cgtccgtaaa gcaggctatg taccggaatc cgtaccgctg
     2761 gaacaccaca tgttcggcat gatgctgggt aaagacggca aaccgttcaa aacccgcgcg
     2821 ggtggtacag tgaaactggc cgatctgctg gatgaagccc tggaacgtgc acgccgtctg
     2881 gtggcagaaa agaacccgga tatgccagct gacgagctgg aaaaactggc taacgcggtt
     2941 ggtattggtg cggtgaaata tgcggatctc tccaaaaacc gcaccacaga ctacatcttc
     3001 gactgggaca acatgctggc gtttgagggt aataccgcgc catacatgca gtatgcttac
     3061 acgcgtgtat tgtccgtgtt ccgtaaggca gaaattaacg aagagcaact ggctgcagct
     3121 ccggtaatca tccgtgaaga tcgtgaagcg caactggcag ctcgcctgct gcagtttgaa
     3181 gaaaccctca ccgtggttgc ccgtgaaggc acgccgcatg tgatgtgtgc ttacctgtac
     3241 gatctggctg gtctgttctc tggcttctac gagcactgcc cgatcctcag cgcagaaaac
     3301 gaagaagtgc gtaacagccg tctgaaactg gcacaactga cagcgaagac gctaaagctg
     3361 ggtctggata cgctgggtat tgagacagta gagcgtatgt aatcgatttt tcgtgagagt
     3421 aaagcctgat cagggttacc tgatcaggct tttttattgc catctaaatg tattctgaaa
     3481 atggacatgc cattgttttc tgactgttgg ataagaggcc agaagcgtaa tatccggccc
     3541 cagggaaacg ataacggttg aatttaagga ataccgcagt gtttaaattt cttgtattaa
     3601 cattaggcat tatctcttgc caggcttacg cagaagatac ggttatagta aacgaccatg
     3661 acatttcagc catcaaagat tgttggcaaa aaaattcaga tgatgatact gacattaacg
     3721 tgatcaaatc atgcctgcga caagaataca atctcgtcga tgcgcaatta aataaagcct
     3781 atggtgaagc ttatcgttat atagaacaag tgccacgcac aggtgtaaaa aaacctgata
     3841 ccgaacaact taacttgctt aaaaaatcac agcgagcctg gctggatttt agggacaaag
     3901 aatgtgaatt aatcctttca aatgaggacg ttcaggattt aagtgaccct tattctgaat
     3961 cagaatggct ctcatgtatg atcatacaga ccaatacgcg aactcgccag ttgcagctat
     4021 accataactc tgaagatttt tatccaagcc ctttgacaag aggataattc acatcttttt
     4081 ggcatgtttt gttgcaagct attcctgata aataattgca acaagacatc gagccttttt
     4141 cactgagtta ttaaacatac tcgcgagcgc gtaatttttt tgtccttcag cgataattca
     4201 caatcacttc attacgttgt acctttagcg gtggaattaa ccgcccaccg cctggcactt
     4261 cccagataaa tcgcaacggt tctgctgccg ctataccgga aaaggccacg gtggttccgc
     4321 tctgcccctc taattcgaca caacgagatt gcgaacacaa gcgaacccgc agtcctgaag
     4381 gtgtcgggcc gataagctga taacgccacg ctaccagcgt catcaatcct gaagcaggtt
     4441 gtcgcgtaga aagaggcgca gacgacatcg actcaccgcg atgatttagc gtaattccta
     4501 cactacttgc ctgccacatc ccctccccgg cggcttgcac cagcagcgga aacaataata
     4561 ttgctaataa ggttctcatt atttgccgcc aattgtcgcc gtcatgcgga tatgtcggtt
     4621 atcagacagt tccagattcg acaggaccac taactgcggc aagctgcggc gcaggaagcg
     4681 agacaataat ggtcgcagcg cgtggttcac caacaatact ggcggcgcac ccagcatctc
     4741 ctgacgggat agcgcttcct gagtttgcgc cagtaaacga tccgccagcc ctggctccag
     4801 tcctcccccg ccctgcagcg cctgtagtaa caaacgttcc agcggtgtat cgaggccaat
     4861 aacatggact tcatctttgc caggaaacca ctgctgggta atcgcccgtc ccaacgccac
     4921 gcgcacgacg gcggttaatt catgtggatc gctttggatg ggcgcatgtt ccgccagcgt
     4981 ttcgagaatg gtgcgcatat cgcgaatcgg tactttttca tcgaggagat tttgcagcac
     5041 tttatgcagt gtggtgagcg tgacgacgcc aggaacgaga tcttccgtca gctttggcat
     5101 ctcctgggcg acgcgatcca acagctgttg cgcctcctga cgaccaaaca gctctgcggc
     5161 atgctggcta atgaggtggt taagatgcgt tgctaccacc gtgctggcct caaccactgt
     5221 gtacccctga atctgcgcct gttcttttag cgcactttca atccagatag cattcaggcc
     5281 aaatgccgga tcgacggtcg cctcaccagg taacgtcccg gcagcggttc cagggttaat
     5341 cgccagccag cgccccggat aagcatcacc actgccaatc tccacgcctt tcatcaaaat
     5401 gcgatagcgg gcaggttgca gatccatatt gtcgcgaatg tgcaccactg gcggcagaaa
     5461 tcccatctcc tgggcaaatt tcttgcggat actgcgtata cggcccaaca actcaccatc
     5521 ctgctggaaa tcgaccatcg ggatcagtcg ataacccact tccattccca gagaatcttc
     5581 cagttgtaca tcgttccacg tcgcttcgac aacggtatta ttctctgcca tttttaccgg
     5641 tttgggttcg gcaggcgctt tttgttcgcg tccgcgtatc caccaggcca gccccagcaa
     5701 tccggcagtg aacagcaaaa ataccaggtt cggcattcca ggcaccaggc cgagtaaacc
     5761 gagcacggcg gcgcttaaca acataacgct tgggttactg aaaagctgat tcaccatctg
     5821 ctcgccaaca tcctgatcgg tgctgacacg cgtaacgatg acccccgcgg cggtagaaat
     5881 caccagcgct ggaatttgtg ccaccagacc gtcgccaatg gtcaatagcg tataactttc
     5941 cgccgcgtgt cccatgctca tgccatgttg cagcacgccg accagcaacc cgccgacaac
     6001 gttaatgacc atgatgagga tcccggcgat ggcatcgccg cgaacaaact tacttgcccc
     6061 gtccattgag ccgtaaaaat cggcttcctg agtcacttcg gagcggcgtt ttttcgcctc
     6121 atcttcacca atcaatccag cgttaaggtc ggcgtcaatc gccatctgct tacccggcat
     6181 accatcgaga acaaagcgcg cacccacttc tgcgatacgc ccggcacctt tggtaatgac
     6241 cataaagttg atgatcacga gaatgacaaa caccacgata ccgatagcga aattgccacc
     6301 aacgaggaag tgaccgaatg cttcgaccac cttcccagcc gccgccgcgc cggtatgccc
     6361 ttccatcaaa atgatacggg ttgaagccac gttaagtgcc agacgcaaca acgtagtaaa
     6421 caacagaatg gtcggaaacg cagcaaactc aagcgtgcgc tgggtaaaca tcgccaccag
     6481 caacaccatg atcgacaagg caatattgaa ggtaaacaac aggtcgagta tgaatgcggg
     6541 cagtggcagc accatcatcg acaagatcaa caggatcaaa atcggtccgg caaggatctg
     6601 ccattgtgtc gatttcaggt ttgcgggcag gcgcagcatc gcggccagat tactcatggg
     6661 tcggtttctc gttaataaaa tccagggctt ccggcaccgg aagatgagta ggttgtacag
     6721 ggcgctgtcc accagccaga cgccagcgtt tcagttgcca gacccaggcc agcacttccg
     6781 ccaccgcggc gtacagttga cccgggattt gttgaccaat ctccgcatgt cgatacagcg
     6841 ctcgcgccag cggcggcgct tcaagcgtcg ggacgttatt ttcagcgcca atttcacgaa
     6901 tgcgcagcgc gaccagccct gcacctttag cgaccacttt cggtgcgctc attttgtttt
     6961 cgtcatactg caacgctacc gaatagtggg tcgggttatt gacaatgaca tccgctttcg
     7021 gcacatcggc catcatccga cgccgtgcag cagctcgctg catctgacgg atccgccctt
     7081 taacatgagg gtcaccttcg ctttgtttga actcatcacg aatatcctgc cgtgacatac
     7141 gcagcttttt caggtggctg aatatttgga aaaagacgtc aaatcccacc attggaatga
     7201 caccaagcac caccagcagt gcgcatagcc ctaccaaatc catcgcatta cccatggcgg
     7261 taatcggaga ctcggccatc aagcgcatca tctgcggcca gtgatgccag agaaaaaacc
     7321 ccgtcacgct gccaaccagg atggttttca aaattgcttt aagcaactcc gcgccagtct
     7381 gagccgagaa catccgttta atgcccggta gcgggttgag tttggaaaac ttcggctgca
     7441 aggatttgcc gctaaatacc agccctccca gcatgaccgg agaaatgagc gccaccagca
     7501 ccacgccgct aatcagcggc agcagcgcca gcatggcttc tctgatcagc agaataatct
     7561 gcccgaggat cagattcggg tcattgataa tactgtgatc aaaatgcagc ccagcggaga
     7621 gcatgcccga caatcgacgg gccagcgaca caccgccaaa ccagataaca ctaacgccca
     7681 ccagcaaaat cagcagtgag gtcagttcac gggaacgcgg gatttgcccc tcttcccgcg
     7741 ctttttctag tcggtgaggt gtgggggctt ctgttttgtc gtcgctctcg tcagacacgt
     7801 cgccaatcct ggaaagagta ttaagcgtga atgatgccag agcgcaaagc gttcaatggt
     7861 ttgagtaagg ggcaaaacag gcgggattta gggcttttgc tgccacacat caagcatagt
     7921 gtgcgtttgt cggatgcggc gtcatcgcct tatcagaccg cctgatatga cgtggtcacg
     7981 ccacatcagg caatacaaat caaaatccaa gactatccaa caaatcgtcc acctgatcct
     8041 gactggctac cacaccggct ttgctggtat cgacctgagg tccattaagc aaactctggt
     8101 tttcacgttt tggacgcgac tcctgttccg ggatgttttc caacagcacc atcagcaact
     8161 ggcgttcgat ctcctgaatg acatccatca tccgcttaat gacctgcccg gtgagatcct
     8221 gaaaatcctg cgccatcatg atttccagca gttgcgcgtt agtaaagctg gtatgcgcgg
     8281 gtacatctgc cagaaattgt cgtgtatctg ttaccagttc acgggcgtcg gcaaggtcaa
     8341 tcggatcggc aaaccagtca tcccaacgtt gggttaacgc ttttgctgat ttctccattt
     8401 gatcctgatg cggttgtgac gcctcaacac tgttcagcgc ccgctccgca gcctgggcgg
     8461 tcatctgcac aacatagtac aaacgatcgc gcgcatcggg gatggcttcc gccgcttcgg
     8521 caatggcctg atccagcccc agttcccgca aactgtcgcg cagcatacgc gtcaggctgc
     8581 cgatgcgcgc aatgatatcg ccagctgaat gctcgtcagc aggtttgatt gatggttgca
     8641 tcatagtcgc atcctcacat gcccagtttc tcaaagattt tgttgagttt ttcctccagc
     8701 gtcgcggcgg taaatggctt caccacatag ccactggccc ccgcttgcgc cgcagcaatg
     8761 atgttctctt tcttcgcttc tgcagtcacc attaacactg gcaatgccga catcgcgcca
     8821 tccgcacgaa ttgttttcag caattccagg ccatccatat tgggcatgtt ccagtcggag
     8881 ataacaaatc cataaccgcc tgcctgcaac ttattgagag cgtcgacgcc atcttccgct
     8941 tcctcaacat tattgaatcc cagctctttc agcaggttac gcactatgcg tcgcatggtg
     9001 gaaaagtcat ccacaaccaa aaatttaagt tctttatccg ccatttcaca ctcctgattt
     9061 aaatacgtat cgcctgtccg gcactaattt ttgccaacat ttgctggctt acctggctaa
     9121 gatcgaccac ttcgcagaca ccacccatat tgatggcctc gcgcggcatg ccgaacacca
     9181 cgcaacttgc ttcgttttgc gcaagggtcc atgcccccgc ctgacgcatc gccaacattc
     9241 ccgccgcgcc gtcgttgccc ataccggtca ggatcacccc aaccgcatta cgccccgcct
     9301 gtttggcgac agaatggaac aacacatcta ccgaaggccg atgacggtta accgccgggc
     9361 catcgtgaat tttgatttgg taatttgcgc cactacgcga cagctccata tgccgatcgc
     9421 ccggcgcaat ataggcatgc cccggcaaga cacgttctcc gtcttcggct tctttaaccc
     9481 cgatctggca aagcttatta agtctgtcgg caaaagagcg ggtgaaaccg ggcggcatat
     9541 gttgggtaat taacaatgcc gggctggaaa gcggcaacgg ttgcagtacg tgacgaattg
     9601 cctcagttcc acccgttgaa gcaccaatcg caatcagttt ttcagaactc aacaacggac
     9661 ccgccttcag cgttgtcggt gctgacaatg gcttatgtgc tgcaaggctc gcctttgctg
     9721 ctgtacgcac cttttcagca atcatttcgc tatacgccag cattccttcg cgaataccca
     9781 gttgcggttt ggtgacaaaa tctatcgccc ccagctccag cgcgcgcagc gtgacttctg
     9841 accctttgcc agtcagggaa gaaaccatca ctacgggcat tggacgcaaa cgcattaatt
     9901 tttcgaggaa atccagtccg tccatccgcg gcatttcaac atccagcgtc agcacatcgg
     9961 gattgaattt cttaatcaag tcacgcgcga ccagcggatc aggcgcggtc gccaccattt
    10021 ccatgtcgct atggctgttg atgatttctg tcatgatctg gcgcatcagt gccgaatcat
    10081 cgacagataa caccctgatt ttgctcatct ttaatcctta cttagcgcat acaccgtctg
    10141 accacgcagc gtgaagcggc gctcaaggtg gctaaagttt tcagagtgac ccgcaaacag
    10201 caatccgtcg ggtttaagga gcggaacaaa gcggcgcaaa atctcctgct gggtattttg
    10261 atcgaagtag atcatgacgt tacgacagaa gatcgcatca aacggccccg gcacggtgta
    10321 ctgtttcgcc agtagattca gcggggcaaa atcaacatag ttcgccagct cctgacgcac
    10381 gcgtaccagc ccttcatgcg gtcccgtccc tcgcatgaaa taccgttgca gttgctgcgg
    10441 cgtcaggttt ttcaactctt catggcgata aataccgctt ctggcttttt ccagcacttc
    10501 ggtgtcgata tcactggcaa acactttcca gcgtccgggc gcggtgccca atgtgtcagc
    10561 cagcgtcatc gcaatgctgt acggctcttc gccggtcgaa gccgccgcgc tccatacgcg
    10621 atactcgcca gaacggcgac gtgcgtgatc cgcgagcaga gggaaatggt gtgcctcacg
    10681 gaaaaatgcc gtcagattcg tggtcagcga attgataaac gcctgccact caccgctgtg
    10741 ctgattagat tccagcaagt tcagataatg accgaaatcc gtcagtccca gcgaacgcaa
    10801 acgacgaacc agtcggttgt aaaccatgtc gcgtttatgg tcagccagaa cgatcccggc
    10861 tcgttgatag atcaattgac ttatccgccg aaaatgcgcg tcggaaagcg ccaggcgctc
    10921 ggtcatctgt aacaataaag acgtttgccc acagggcaga gatgaagtca tagcgccttc
    10981 tcaatcactt caggatacca ctggcgcaat ttgtaactgc gccgactcat gtcgtgctac
    11041 ttcatgttct tcaagggtaa ataccgccac acgcgacgaa agatggtcgg cctgattcgc
    11101 cagttgttcc gttgccaccg ccgcttcttc taccagcgag gcgttctgct gagtcacctg
    11161 atccatctgg ctgacagcct gtgcaacctg ctcaatcccc cgctgttgtt cttccgacgc
    11221 agaggcaatt tctcccataa tgtcgttcac gcgggtcacc gaactgacaa tatcgatcat
    11281 ggtcgcggcg gcgttattca ccagtttcga accctgctgg acacgattga ctgactcttc
    11341 gatcagccct ttgatctctt ttgctgcctg cgcgctacgg ctggcaagat tgcgcacttc
    11401 acctgccact accgcaaaac cacgcccctg ctctccggcg cgagccgctt ccactgccgc
    11461 attcagggcc agaatattgg tctggaaagc aattccgtcg ataacgctga taatgtcgcc
    11521 aattttttgc gagctggtgg cgatctcctg catggtgtga gtcatggtac tgacctgaac
    11581 accgcccgcc tgcgccgttg tcgcggcatt ttttgccagt tccgacgcct gtcgtgcgtt
    11641 atcggcgttt tgccctaccg tggcggttaa ttgctccata ctggcggccg tttgtgccag
    11701 cgatgccgcc tgctgttcgg tacgacttga gagatcgtta ttgcctgcga caatctccgc
    11761 gataccaatg tgcatctcct ggcttccctt acgcacatca cttaccgtcc cacgcaaagc
    11821 ctgctgcatg gtcttcagac tggcaaaaat ggcggtgatc tcattacgac catataccgc
    11881 aatcggacgc gccagattac ccgcagcaat gctgtcaaaa tggctaccga taatggccag
    11941 tggttgaaca atcatcttgc gcgtccacca cagcgcactg ctgatgtaga ttgccgcaac
    12001 aataatcatg ctgataaaca ccagtgccga aatctgatag ttacgctggc tttgcgcact
    12061 ggcggcttcc agcacatggt tgatctccat ctgccaggcc tcaaagttaa cgtcaaacgc
    12121 attctgtgat ccctgcaccg gcgcagtgag gaaatccgaa agttgattac tttcaagcca
    12181 ggtcgcctga tgttcgagat cgttgtgcca gcgggcaaaa cttttctccg tctctttttg
    12241 caatgccctg acgtgctcgt tgcccgcagt catcgccata aaacttttaa acagcgtggt
    12301 ggattgcgtc agactggcgc gcgccgtcgt catcaacgtt ttaatgtcat ccgccggata
    12361 gctaagtgcc gtcagagtgc ccgctttgtt cagcgcggta ctggcctgta acattaccgc
    12421 ccgagtttgc gctaatgccg cacgttgctg attgctctgc tcgacctgat tcagtcgttg
    12481 caaatcgtcg cgaaatgccc aaaaagacat gccgttactg ccaatctgca agatcccgca
    12541 gagaatcaaa attaaaaaca gcgtggtcga aattcgaata cgattaaaca tcaacgctcc
    12601 ccatcaggcg gcaatgaccg cgttagtaaa tactcgtcaa aatgtttccc agtttggatc
    12661 ttgttcagca attcgcagtc gtggctgagc cggtggttgc tcactggcag gacgggatgg
    12721 tgtttgcggt ttattggtga gtgggctggc tgccagacgg aacgcggaaa ccgcttgcgt
    12781 taaacgactc gcctgttctt ccagcgcagc ggcggcggca gctgattcct gcaccagcga
    12841 tgcgttctgt tgcgtgacgc gatccatttc cgaaaccgcc aatgcgactt gatcgatgcc
    12901 acgactctgt tcatccgatg ccgatgcaat ctcgcccata atgtcagtca cgcgagtgac
    12961 agcattgacg atattgttca ttgtttcccc ggcgctttcg accagcaccg aaccggtatc
    13021 aacgcgtgag acggagtctt caatgagggc tttgatctct tttgccgcct gggcgctgcg
    13081 actggcaaga ttacgcactt cacccgccac cacggcaaaa ccacggccct gttcacccgc
    13141 acgcgcggct tcaaccgcgg cattcagcgc gaggatatta gtctggaagg caataccgtc
    13201 gataacgctg ataatgtcgg caattttctt cgaactatcg gcgatctcat gcatcgtttt
    13261 cactacgcca tccaccactt tgccgccgtg ctgggcggtg tcggaggcac tttgcgccag
    13321 ttgcgaggcc tggcgggcgt tatcggcgtt ttgcttcact gtcgcggtga gctgctccat
    13381 gctggcggca gtttcttcca gcgcggatgc ctgctgttca gtacgggagg aaagatcggt
    13441 gttgcccgcc gcaatttcac gggtaccggc atagatggca tctgaacctt cgcggacatg
    13501 agtgacggtg tcagtcaaag agcgttgcat atgtgaaacg ctctgcgcca ggtcgcccat
    13561 ttcactgcgc ccgtcaatgg tcagggtatt cgccaggtta ccaccggcga tttcgcgaat
    13621 gtgagcaata atttttgcca gcggagtaag caacatacgg cgaatgccgt accacgccac
    13681 cagcagaatc aataccacca ccagcgcgat aaccgccagt tgccactggg caaatcggta
    13741 atcatctgcg ttgtcagtga cgatatcgcg atacagtttt tcactgctga gggcgtactg
    13801 agcaaacgct tcgcccattg cattttgcat tccctgggtt ggctgagcga aataagctcc
    13861 agtattgcca taa
//
LOCUS       DAPHDW010000121        13488 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.123, whole genome shotgun sequence.
ACCESSION   DAPHDW010000121 DAPHDW010000000
VERSION     DAPHDW010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.123"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            7..1215
                     /locus_tag="RH057_003457"
     CDS             7..1215
                     /locus_tag="RH057_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3129774.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKGLERAYKNFFQKRAAFPR
                     FKKRGQNDAFRYPQGVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGKWY
                     ISIQTESEVSTPVHPSASMVGLDAGVAKLATLSDGTVFEPVNSFQKNQKTPARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGGMVQSGRPLKQ
                     EPTEMIQATA"
     gene            complement(1255..2469)
                     /gene="ydcO"
                     /locus_tag="RH057_003458"
     CDS             complement(1255..2469)
                     /gene="ydcO"
                     /locus_tag="RH057_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BenE family transporter YdcO"
                     /protein_id="HDV3129775.1"
                     /translation="MLRCLYAITHEVTMRLFSIPPPTLLAGFLAVLIGYASSAAIIWQ
                     AAIVAGATTAQISGWMTALGLAMGVSTLALTLWYRVPVLTAWSTPGAALLVTGLQGLT
                     LNEAIGVFIVTNALIVLCGITGLFARLMCIIPHSLAAAMLAGILLRFGLQAFASLDGQ
                     FTLCGSMLLVWLATKAVAPRYAVIAAMIIGIVIVIAQGDVVTTDVVFKPVLPTYITPD
                     FAFAHSLSVALPLFLVTMASQNAPGIAAMKAAGYSAPVSPLIVFTGLLALVFSPFGVY
                     SVGIAAITAAICQSPEAHPDKDQRWLAAAVAGIFYLLAGLFGSAITGMMAALPVSWIQ
                     MLAGLALLSTIGGSLYQALHNERERDAAVVAFLVTASGLTLVGIGSAFWGLIAGGVCY
                     VVLNLIADRNRY"
     gene            2522..3058
                     /gene="sutR"
                     /locus_tag="RH057_003459"
     CDS             2522..3058
                     /gene="sutR"
                     /locus_tag="RH057_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SutR"
                     /protein_id="HDV3129776.1"
                     /translation="MENLARFLSTTLKQLRQQRGWSLSRLAEATGVSKAMLGQIERNE
                     SSPTVATLWKIATGLNVPFSTFISPPQSATPSVYDPQQQAMVITSLFPYDPQLCFEHF
                     SIQMASGAISESTPHEKGVIEHVVVIDGQLDLCVDGEWQTLNCGEGVRFAADVTHIYR
                     NGGEQTVHFHSLIHYPRS"
     gene            3131..5092
                     /gene="rlhA"
                     /locus_tag="RH057_003460"
     CDS             3131..5092
                     /gene="rlhA"
                     /locus_tag="RH057_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA 5-hydroxycytidine C2501 synthase"
                     /protein_id="HDV3129777.1"
                     /translation="MTVSSHRLELLSPARDAAIAREAILHGADAVYIGGPGFGARHNA
                     SNSLKDIAELVPFAHRYGAKIFVTLNTILHDDELEPAQRLITDLYQTGVDALIVQDMG
                     ILELDIPPIELHASTQCDIRTVEKAKFLSDVGFTQIVLARELNLDQIRAIHQATDATI
                     EFFIHGALCVAYSGQCYISHAQTGRSANRGDCSQACRLPYTLKDDQGRVVSYEKHLLS
                     MKDNDQTANLGALIDAGVRSFKIEGRYKDMSYVKNITAHYRQMLDAIIEERGDLARAS
                     SGRTEHFFVPSTEKTFHRGSTDYFVNARKGDIGAFDSPKFIGLPVGEVLKVAKDHLDV
                     AVTEPLANGDGLNVMIKREVVGFRANTVEKTGENQYRVWPNEMPADLHKIRPHHPLNR
                     NLDHDWQQALTKTSSERRVAVDIELGGWQEQLILTLTSEEGVSITHTLDGQFDEANNA
                     EKAMNNLKDGLAKLGQTLYYARDVQINLPGALFVPNSLLNQFRREAADMLDAARLASY
                     QRGSRKPVADPAPVYPQTHLSFLANVYNQKAREFYHRYGVQLIDAAYEAHEEKGEVPV
                     MITKHCLRFAFNLCPKQAKGNIKSWKATPMQLVNGDEVLTLKFDCRPCEMHVIGKIKN
                     HILKMPLPGSVVASVSPDELLKTLPKRKG"
     gene            complement(5184..5414)
                     /locus_tag="RH057_003461"
     CDS             complement(5184..5414)
                     /locus_tag="RH057_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncJ family protein"
                     /protein_id="HDV3129778.1"
                     /translation="MFTKALSVVLLTCALFSGQLMAGHKGHEFVWVKNVDHQLRHEAD
                     SDELRAVAEESAEGLREHFYWQKSRKPEAGQR"
     gene            5636..5812
                     /gene="hicA"
                     /locus_tag="RH057_003462"
     CDS             5636..5812
                     /gene="hicA"
                     /locus_tag="RH057_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin HicA"
                     /protein_id="HDV3129779.1"
                     /translation="MKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIK
                     EPLRKAILKQLGLS"
     gene            5858..6274
                     /gene="hicB"
                     /locus_tag="RH057_003463"
     CDS             5858..6274
                     /gene="hicB"
                     /locus_tag="RH057_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HicB"
                     /protein_id="HDV3129780.1"
                     /translation="MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALL
                     TAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKVLLLNAFLQSEITQQELARRIGK
                     PKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV"
     gene            6353..7759
                     /locus_tag="RH057_003464"
     CDS             6353..7759
                     /locus_tag="RH057_003464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HDV3129781.1"
                     /translation="MKKYQQLAEQLREQIASGIWQPGDRLPSLRDQVALSGMSFMTVS
                     HAYQLLESLGYIIARPQSGYYVAPQAIKMPKAPVIPVTRDEAVDINTYIFDMLQASRD
                     PSVVPFASAFPDPRLFPLQQLNRSLAQVSKTATAMSVIENLPPGNAELRQAIARRYAL
                     QGITISPDEIVITAGALEALNLSLQAVTEPGDWVIVENPCFYGALQALERLRLKALSV
                     ATDVKEGIDLQALELALQDYPVKACWLMTNSQNPLGFTLTPQKKAQLVALLNQYNVTL
                     IEDDVYSELYFGREKPLPAKAWDRHDGVLHCSSFSKFLVPGFRIGWVAAGKHARKIQR
                     LQLMSTLSTSSPMQLALVDYLSTRRYDAHLRRLRRQLAERKQRAWQALLRYLPAEVKI
                     HHSDSGYFLWLELPEPLDAGELSLAALTHHISIAPGKMFSTGENWSRFFRFNTAWQWG
                     EREEQAVKQLGKLIQERL"
     gene            8004..9149
                     /locus_tag="RH057_003465"
     CDS             8004..9149
                     /locus_tag="RH057_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HDV3129782.1"
                     /translation="MSKTFARSSLCALTMTIMTAHAAEPPTNLDKPEGRLDIIAWPGY
                     IERGQTDKQYDWVTQFEKETGCAVNVKTAATSDEMVSLMTKGGYDLVTASGDASLRLI
                     MGKRVQPINTALIPNWKTLDPRVVKGDWFNVGGKVYGTPYQWGPNLLMYNTKTFPTPP
                     DSWQVVFVEQNLPDGKSNKGRVQAYDGPIYIADAALFVKATQPQLGISDPYQLTEEQY
                     QAVLKVLRAQHSLIHRYWHDTTVQMSDFKNEGVVASSAWPYQANALKAEGQPVATVFP
                     KEGVTGWADTTMLHSEAKHPVCAYKWMNWSLTPKVQGDVAAWFGSLPVVPEGCKASPL
                     LGEKGCETNGFNYFDKIAFWKTPIAEGGKFVPYSRWTQDYIAIMGGR"
     gene            9167..10180
                     /locus_tag="RH057_003466"
     CDS             9167..10180
                     /locus_tag="RH057_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HDV3129783.1"
                     /translation="MTYAVEFDNVSRLYGDVRAVDGVSIAIKDGEFFSMLGPSGSGKT
                     TCLRLIAGFEQLSGGAISIFGKPASNLPPWERDVNTVFQDYALFPHMSILDNVAYGLM
                     VKGVNKKQRHAMAQEALEKVALGFVHQRKPSQLSGGQRQRVAIARALVNEPRVLLLDE
                     PLGALDLKLREQMQLELKKLQQSLGITFIFVTHDQGEALSMSDRVAVFNNGRIEQVDS
                     PRDLYMRPRTPFVAGFVGTSNVFDGLMAEKLCGMTGSFALRPEHIRLNTPGELQANGT
                     IQAVQYQGAATRFELKLNGGEKLLVSQANMTGEELPATLTPGQQVMVSWSRDVMVPLV
                     EER"
     gene            10181..11122
                     /locus_tag="RH057_003467"
     CDS             10181..11122
                     /locus_tag="RH057_003467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3129784.1"
                     /translation="MAMNVLQSPSRPGLGKVSGFFWRNPALGLFLLLLGPLMWFGIVY
                     FGSLLTLLWQGFYTFDDFTMSVTPELTLANIRALFNPANYDIILRTLTMAVAVTIASA
                     ILAFPMAWYMARYTSGKMKAFFYIAVMLPMWASYIVKAYAWTLLLAKDGVAQWFLQHL
                     GLEPLLTAFLTLPAVGGNTLSTSGLGRFLVFLYIWLPFMILPVQAALERLPPSLLQAS
                     ADLGARPRQTFRYVVLPLAIPGIAAGSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQ
                     QGAIGNMPMAAAFTLVPIVLIALYLAFVKRLGAFDAL"
     gene            11112..11906
                     /locus_tag="RH057_003468"
     CDS             11112..11906
                     /locus_tag="RH057_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HDV3129785.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLMALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            11928..13352
                     /gene="patD"
                     /locus_tag="RH057_003469"
     CDS             11928..13352
                     /gene="patD"
                     /locus_tag="RH057_003469"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HDV3129786.1"
                     /translation="MQHKLLINGELVSGEGEKQPVYNPATGDVLLEIAEASAEQVDAA
                     VRAADAAFAEWGQTTPKVRAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINVLFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
BASE COUNT         3292 a         3310 c         3621 g         3265 t
ORIGIN      
        1 tggcctgtga aacgattaca ggcatttaaa ttccagttaa gacccggtgg tcaacaggag
       61 cgtgaaatga ggcgcttcgc aggcgcatgt cgtttcgttt tcaatcgtgc tctggcgctt
      121 cagaatgaga atcatgaggc cggtaataaa tacatccctt acgggaaaat ggcttcctgg
      181 ctggttgagt ggaaaaacgc cactgaaacg caatggctta aagattcgcc ctcacagcca
      241 ttgcaacagt cactgaaagg ccttgagcgg gcttacaaaa acttcttcca gaagcgggct
      301 gcttttcccc gattcaaaaa gcggggacag aatgatgcat tccgctaccc gcagggcgtt
      361 aagctcgatc aggaaaacag ccgtattttt ctgccgaaac tgggctggat gcgctaccgg
      421 aacagccgtc aggtcacggg agttgtgaaa aatgtcactg tcagccagtc ctgcggtaag
      481 tggtacatca gtattcagac agaaagtgaa gtatcaactc cggttcaccc ttcagcatca
      541 atggtcgggc tggatgctgg cgtggctaaa ctcgccacgc tgtcagatgg cacagtcttt
      601 gagcctgtaa acagttttca gaaaaaccag aagacgccgg cgagacttca gcgacagtta
      661 agccgcaagg tcaaattcag caacaactgg cagaagcaga aacgcaaaat acagcgactg
      721 cattcctgta tcgcaaatat ccgcagggac taccttcaca aagtcacaac gaccgtcagc
      781 aaaaaccacg caatgatagt cattgaggat ttgaaggtca gcaacatgtc aaagtcagca
      841 gcgggtacgg tcagccagcc ggggcgcaat gtccgggcaa aatcaggttt aaaccgttcg
      901 atactggatc agggctggta tgaaatgcgc cgccagcttg aatacaagca gctctggagt
      961 ggcggtcagg tgcttgctgt tccgccagcg tatacaagcc agcgttgcgc gtgctgtggt
     1021 catacagcga aagaaaatcg tctgtcacaa agtaaattca gatgccaggt atgtggatat
     1081 acagcgaacg ccgatgtaaa cggcgctcgt aacattttag cggcggggca cgccgttctt
     1141 gcctgtggag ggatggtgca gtcaggccgc ccgttgaagc aggaacccac cgaaatgatt
     1201 caggcgacag cctgaacgta gcagggatcc acgtccttca gggcgtggag gatgtcaata
     1261 tcggtttctg tcagcgatta aattcaacac cacgtaacaa acgcctccgg caattaatcc
     1321 ccagaacgca gaaccaatcc cgaccagcgt caatccactt gccgttacca gaaatgccac
     1381 caccgccgcg tcgcgctcac gctcattatg cagcgcctga tacaaacttc cgccaatggt
     1441 actcaacagc gccagacctg ccagcatctg gatccaactt acgggcaggg cagccatcat
     1501 cccggtaatg gcactaccaa acagacctgc gagcaaatag aaaatgcctg caacggcagc
     1561 tgccagccaa cgttgatctt tatccggatg cgcttccggg ctttggcaaa tagccgcggt
     1621 gattgccgca ataccgacgg aataaacgcc gaaaggggaa aaaaccagtg ccagcaatcc
     1681 agtaaataca attaatggcg aaacaggagc cgaatatccg gcggctttca ttgctgcgat
     1741 acccggtgcg ttttgcgatg ccatcgtcac cagaaaaagg gggagtgcaa cgctcaggct
     1801 gtgagcaaac gcaaaatcag gggtaatata agtggggaga acgggtttaa agacaacatc
     1861 agttgtgaca acgtcacctt gcgcgataac gatcacgatc ccaataatca tcgcggcaat
     1921 taccgcatag cgcggcgcaa cggccttggt tgccagccat accagcaaca tactgccaca
     1981 caacgtaaat tgaccgtcca gactggcaaa cgcctgtaaa ccaaagcgta ataaaatccc
     2041 ggcaagcatt gccgccgcaa gcgagtgcgg aataatgcac atcagacgag caaagagtcc
     2101 cgttatgccg cagaggacta ttaacgcgtt ggtgacaata aaaacgccga tggcttcgtt
     2161 aagtgttagt ccctgcaatc cggtgaccaa caaagccgcg ccaggcgttg accatgcggt
     2221 gagaacaggt acgcgatacc ataatgtcag agccagcgta ctgacgccca ttgccagccc
     2281 cagcgccgtc atccagccag agatttgtgc agtggtggct ccggcgacaa tcgctgcttg
     2341 ccagattatt gccgctgaac tggcgtagcc aattaatacc gccagaaacc ccgccagtag
     2401 cgtgggtgga ggaatagaaa acagacgcat agtcacctcg tgcgttatag cgtacaggca
     2461 ccgtagcatt tgtccgttat aacgcacaag tgataaactt ccgttttgcc ggaggagtcg
     2521 catggaaaat ctcgctcgct ttttatccac cacacttaaa caactacgcc agcagcgcgg
     2581 ctggagtctt tcgcgactgg cagaagcgac gggcgtttct aaagcaatgt tagggcagat
     2641 tgagcgtaat gagtccagtc cgacggtcgc gacattatgg aaaattgcca ccgggttgaa
     2701 cgtaccattt tcaacattta tttctccgcc gcagtccgct acgccttcag tttatgatcc
     2761 acaacagcag gcgatggtga tcacttcgct gtttccttat gatccacaac tctgcttcga
     2821 acacttctcg attcagatgg catctggtgc aataagcgaa tcgacgcccc atgagaaggg
     2881 ggtaatcgaa catgtcgtcg tcattgatgg acaacttgat ctgtgcgttg atggggaatg
     2941 gcagaccctt aattgtgggg aaggcgttcg atttgctgca gacgtcacgc atatctatcg
     3001 taatggtggg gagcaaaccg tacattttca ttccctcatc cattacccgc gcagttaagc
     3061 gggaaaacta tttcgcaacg tcgcgcttgt ggctaaaata gccgccattt ttcagctact
     3121 ggataagaat gtgaccgtat cttctcatcg acttgaactg ttaagcccgg cacgcgatgc
     3181 cgccattgcc cgcgaagcta ttttgcacgg tgccgatgct gtttatatcg gcggccctgg
     3241 ttttggtgcc cgtcataatg ccagtaatag cctgaaagat attgccgagc tggtgccgtt
     3301 tgcccatcgt tatggtgcaa aaattttcgt cacgcttaac accattttgc atgatgatga
     3361 gctggaaccc gcgcaacggc tgattactga cctctaccag accggtgtcg atgcgctgat
     3421 tgttcaggat atggggattc tggaacttga tattccgccg attgaactgc acgccagtac
     3481 gcagtgcgac attcgtacag ttgaaaaagc gaagttcctc tctgatgttg gcttcacgca
     3541 gattgtgctg gcgcgagagc tgaatcttga tcagatccgc gcgattcacc aggctacgga
     3601 cgcgaccatt gaattcttta ttcatggggc actgtgcgtg gcctattcgg gtcagtgcta
     3661 catttctcat gcgcaaacag ggcgtagcgc caaccgtggc gattgctcgc aggcgtgccg
     3721 tttgccatac acattgaaag acgatcaggg gcgggtggtt tcctatgaaa aacatctgct
     3781 gtcgatgaaa gataacgatc agactgccaa cctcggcgcg ctgattgatg ctggtgtacg
     3841 ctccttcaag attgaagggc gttacaaaga tatgagctac gtgaagaata tcaccgccca
     3901 ttatcgccag atgcttgatg ccattattga agaacgtggc gatctggcgc gcgcttcatc
     3961 aggtcgtact gaacatttct ttgttccatc gacggaaaag actttccacc gtggtagcac
     4021 agattatttt gtgaatgccc gtaaaggcga tattggcgcg ttcgattcgc cgaaatttat
     4081 cggcctgccg gtgggtgaag tattgaaagt ggcgaaagat catctcgatg ttgccgtcac
     4141 cgagccactg gcaaatggtg atggcctgaa cgtgatgatt aaacgtgaag tcgtcggttt
     4201 tcgtgccaat acggtcgaga aaaccggaga aaatcagtac cgcgtctggc ccaatgaaat
     4261 gccagcagat ttgcacaaaa ttcgtccaca tcacccacta aaccgtaatc ttgatcatga
     4321 ctggcagcag gcactgacaa aaacctccag cgaacgtcgg gtggcggtag acattgaact
     4381 gggcggctgg caggaacaac tgattctgac cctcaccagt gaagagggtg tcagcatcac
     4441 gcatacgctg gacgggcagt tcgacgaagc caataacgcc gaaaaagcaa tgaataatct
     4501 gaaggatggt ctggcaaaac tggggcaaac cctctattac gcccgcgatg tgcaaattaa
     4561 tttgccgggg gcgctgtttg taccaaacag tctgttaaac cagttccgcc gtgaagctgc
     4621 tgacatgctg gatgctgcgc gtcttgccag ttaccagcgc ggcagccgta aaccggttgc
     4681 tgatcctgcg ccggtttatc cgcaaacgca tctgagtttc ctcgcgaacg tatacaacca
     4741 gaaagcgcgt gaattttatc atcgctatgg tgtgcagctg attgacgcgg cgtatgaagc
     4801 acatgaagag aagggcgaag tcccggtgat gatcaccaag cattgtctgc gctttgcctt
     4861 taatctgtgc ccgaaacagg cgaaaggcaa tatcaaaagc tggaaggcga cgccaatgca
     4921 actggttaac ggcgatgaag tattaacgct aaagtttgat tgccgcccat gcgagatgca
     4981 cgtcattggc aaaatcaaaa atcacatact gaaaatgccg ttaccgggaa gcgtagtggc
     5041 atccgtaagt ccggatgagc tgctgaaaac attgcctaag cgaaaagggt aaaacgccag
     5101 ttttctggtt actcacaact cattgaatct gcatgatatt gcctgccggg taaggcgtct
     5161 cgccgcatcc ggcaacaaat gactcaacgt tgtcccgctt ctggtttgcg cgatttttgc
     5221 cagtaaaaat gctcgcgcaa accttctgcc gactcttccg ccacagcacg caattcatcg
     5281 ctgtccgctt catgacgcag ctgatgatcc acattcttta cccacacaaa ttcatgtcct
     5341 ttgtgccctg ccatgagttg tcctgagaac agcgcacacg ttaataagac aaccgataac
     5401 gccttcgtaa acatcctgcc acctttttct taccttttgc cgctatgatg ccgatcgttt
     5461 cttgaggtta ttattcagtt ttgcaaatta gcgcaaagaa attctggaat cttccttcct
     5521 gattttgcat ggcattctgc cgttgcggcg atttagtgct attttcgagc aaattacaca
     5581 cggaggtaaa caataatgtt tactttgttg atatactcag cggcagggag gcgatgtgaa
     5641 acaaagcgag ttcagacgtt ggctcgaatc tcagggcgtc gatgtagcga atggcagcaa
     5701 ccatttgaaa ctcaggtttc atgggaggcg cagtgtcatg ccgcgtcacc cctgcgatga
     5761 gattaaagaa ccattgcgta aagcaatcct gaaacaactc ggtttgagtt aatcgccaat
     5821 taaaaaggtt aatgacatgc gagggacagt cgaaattatg cgttatcccg tcactcttac
     5881 acccgcgccg gaaggcggtt atatggtttc ttttgtggat atccctgaag cgttgacgca
     5941 gggcgaaact gtcgctgaag cgatggaagc ggcaaaagat gctttactga ccgcatttga
     6001 tttttatttt gaagataacg agcttatccc tttaccttcg ccattaaata gtcatgatca
     6061 ctttattgaa gtacctttga gcgtcgcctc taaggtattg ctgttaaatg cttttttaca
     6121 gtcagaaatc actcagcaag agttagccag gcgaattggc aaacctaaac aggagattac
     6181 tcgcctattt aacttgcatc atgcgacaaa aatcgacgcc gtccagctcg cggcaaaggc
     6241 gcttggcaaa gagttatcgc tggtgatggt ttaattacag ttaacgaaaa gttgtcattt
     6301 ttaacaactg atatagactg ccgaatcatc tgcacataat tacgattcga taatgaaaaa
     6361 ataccagcag cttgcagaac aattacgcga gcagattgcg tcgggtatct ggcaacccgg
     6421 cgatcgtttg ccttcgttgc gtgaccaggt ggcgctttct ggcatgagct ttatgactgt
     6481 cagccatgcc tatcagttgc tcgaaagtct gggatatatt atcgcgcgac cgcagtcggg
     6541 ttattacgtt gcgccacagg caataaaaat gccgaaagcg ccagtcattc cagtcactcg
     6601 agatgaagca gtcgatatca acacttatat ttttgatatg ttgcaggcca gtcgcgatcc
     6661 gtcggtcgtt ccgtttgcct cggcctttcc cgacccgcga cttttccccc tccaacaact
     6721 aaaccgctcg ctggcgcagg taagcaaaac cgccacagcg atgagcgtga ttgaaaactt
     6781 accgccagga aacgcagaac tgcgtcaggc tattgctcgt cgctatgcct tacagggcat
     6841 caccatttct cctgatgaaa ttgtcattac tgccggggcg ttagaggcat taaacctcag
     6901 tttgcaagcg gtaactgaac cgggcgattg ggtgatagta gagaatcctt gtttctacgg
     6961 tgcgttgcag gcgctggagc ggctacggct gaaggcgtta tcggtggcga cggatgttaa
     7021 agaagggatc gatcttcagg cgctggaact ggcgttgcag gattatccgg tgaaagcgtg
     7081 ctggctgatg actaatagcc agaacccact cggatttacc ttaacgccgc aaaaaaaagc
     7141 acaactggtg gcgttactca atcagtacaa cgtaacgctg attgaagatg acgtttacag
     7201 cgaactttat tttggacggg aaaaaccgct gcctgcgaaa gcgtgggatc gccacgatgg
     7261 cgttttgcat tgctcttcgt tttcgaaatt tctggtgcct ggttttcgta ttggttgggt
     7321 cgccgccgga aaacatgcac gtaaaattca acgcttgcag ttgatgagta cgctttccac
     7381 cagctcaccg atgcaacttg cgctggtgga ttacctttcc acgcgccgat acgacgccca
     7441 tcttcgtcgc ctgcgtcgcc agcttgcgga acgtaaacaa cgtgcctggc aggcactgct
     7501 gcgttatctg cctgcggaag ttaaaattca tcatagtgac agcggttatt ttctctggct
     7561 ggagctcccc gagccgttag atgccggcga attaagcctg gcggcactga cgcatcatat
     7621 cagtattgcg ccgggtaaaa tgttttctac cggtgaaaac tggtcacgtt tttttcgttt
     7681 taataccgcg tggcagtggg gagaacgtga agaacaggcg gtaaaacaat taggcaaact
     7741 tattcaagaa cggctgtaat agcgtttaat ttaattcctc ttagattggg taatatgaat
     7801 ttcgaatagc agtcatattt cctaacccct tgtctatact ccagaagata accttacaga
     7861 cggcataatg cgcggtagct cacaacctga ttaaattttc tcaggggcga aggtgtgcct
     7921 gcaagccgcc gtctatggtt aaacaaggag atatttttac ggcacggcgg ctgaacaatt
     7981 aattacgaca ggagtaagac cttatgagca agacatttgc ccgcagcagc ctgtgtgcgc
     8041 tcaccatgac aataatgacc gctcacgccg ccgaaccacc taccaattta gataaaccgg
     8101 aagggcgact ggatattatc gcctggccgg gatacatcga acgcggacaa actgataaac
     8161 aatacgactg ggtaacgcaa tttgaaaaag agacaggctg cgcggtgaat gtgaaaaccg
     8221 ccgcgacttc cgatgaaatg gtcagtctga tgaccaaagg gggttacgat ctggttacgg
     8281 catccggcga tgcctcgctg cgtttgatta tgggcaaacg cgtgcagccg attaataccg
     8341 ccttgattcc caactggaaa acgctcgatc cgcgcgtggt taaaggcgac tggtttaatg
     8401 ttggcggcaa agtttacggc acaccttacc aatgggggcc gaacctgctg atgtacaaca
     8461 ctaaaacctt cccgacgccg ccggatagct ggcaagtggt ttttgttgag caaaatctgc
     8521 cggacggcaa gagcaataaa ggccgcgttc aggcttatga tggccctatc tacattgcgg
     8581 acgctgcgtt gttcgttaaa gccactcagc cgcagttggg catcagcgat ccgtatcaac
     8641 taaccgaaga acagtaccag gcggtgctga aagtgctgcg cgctcaacac agtttgatcc
     8701 atcgctactg gcatgacact accgtgcaaa tgagcgattt caaaaacgag ggtgtggttg
     8761 cttccagtgc ctggccctat caggccaacg ccctgaaagc cgaaggccag cctgttgcta
     8821 ccgttttccc gaaggagggt gttaccggtt gggctgatac caccatgctg catagcgaag
     8881 cgaaacatcc ggtttgcgcc tacaaatgga tgaactggtc attaacgcca aaagtgcagg
     8941 gcgatgtggc ggcctggttt ggctcgttac cggtagtgcc ggaagggtgt aaagccagtc
     9001 cgttattagg cgaaaaaggt tgtgaaacca acggttttaa ctatttcgac aaaatcgcct
     9061 tctggaaaac gcctatagca gaagggggca agtttgttcc ctacagtcgc tggacgcagg
     9121 attacattgc cattatgggc ggtcgctaac ttcgctgggg tgctttatga cgtacgcagt
     9181 ggagtttgac aacgtctcgc ggttgtacgg tgacgtgcgc gcagtagatg gcgtcagtat
     9241 tgcgataaaa gatggtgagt tcttctctat gctggggccg tccggctccg gcaaaaccac
     9301 ctgcctgcgc ctgattgctg gcttcgaaca gctttccggc ggggctatct ctatctttgg
     9361 taaacccgcc agcaatctgc caccgtggga gcgggacgtg aatactgtct ttcaggacta
     9421 cgcgctattt ccgcatatgt cgattcttga caatgtcgcc tatgggctga tggtcaaagg
     9481 cgtgaataaa aagcagcggc acgcaatggc gcaagaggcg ctggagaaag tggcgttggg
     9541 gtttgtacat caacgtaaac cgtcacaact ttctggtggt cagcgccagc gggttgctat
     9601 cgccagagca ttggtgaatg aaccgcgcgt attgctgttg gatgaaccgc tcggcgcact
     9661 ggatctcaaa ttgcgtgagc agatgcagct ggaactgaaa aaactgcaac agtctctcgg
     9721 tatcactttt atcttcgtta cccacgatca gggcgaagcg ttatcgatgt ccgatcgtgt
     9781 ggcggttttc aataatggac gcattgagca ggtcgattcc ccgcgcgatc tctatatgcg
     9841 cccgcgcacg ccgtttgttg ccggattcgt cgggacatcc aacgtttttg atggactgat
     9901 ggcagagaaa ctttgtggca tgacgggaag cttcgccctg cgaccggaac atatccgcct
     9961 caacacccct ggtgaactgc aggccaatgg cacgatccag gcggtgcaat atcagggcgc
    10021 ggcaactcgt tttgaactga aattgaacgg cggtgaaaaa ctgcttgtga gtcaggccaa
    10081 tatgacaggc gaagaactgc ctgccacgct cacgcccgga caacaggtga tggtttcctg
    10141 gtcgcgtgat gtgatggtgc cgctggttga ggagaggtga atggcgatga atgtattgca
    10201 atcaccttca cgtccaggtc tgggtaaggt gtccggtttc ttctggcgta atccggcgct
    10261 ggggctgttt ttactgctgc ttggcccgct aatgtggttt ggcattgtct atttcggctc
    10321 gctgctgaca ctgttatggc agggatttta tacttttgac gatttcacca tgtcggtaac
    10381 gccggaactg acgctggcga atatccgtgc gctgtttaat ccggcgaatt acgacatcat
    10441 tctccgcacg ctgactatgg ctgtggcagt cactatcgcc agcgccattc tggcttttcc
    10501 aatggcgtgg tatatggcgc gctataccag cgggaaaatg aaagcttttt tttatattgc
    10561 ggtaatgttg ccgatgtggg cgagctacat tgttaaagcc tatgcctgga cgttattgct
    10621 ggcaaaagat ggcgtggctc agtggttttt acaacatctt gggctggaac cactgctgac
    10681 tgcgttcctt acattacctg cggtgggcgg aaatacgctg tcaacttccg ggctggggcg
    10741 ctttctggtg tttctctata tctggttgcc gttcatgatc ctgcccgttc aggcggcgct
    10801 tgagcgtttg ccgccgtcat tgttgcaggc gtcggctgat ctcggcgcac gtccacgaca
    10861 aacctttcgc tatgtggtgc tgccgctggc aatcccgggt attgccgctg gctctatctt
    10921 taccttctca ctcacactgg gcgattttat cgtcccgcag ctggttggtc ctccaggata
    10981 ttttatcggc aatatggttt attcccagca gggggcgatt ggcaatatgc cgatggcggc
    11041 ggcattcacc ctggtgccga ttgttctcat cgcactgtac ctggcgttcg tgaaacgtct
    11101 gggagcgttc gatgcactct gaacgcgcac cgtttttcct caaactggcg gcctggggcg
    11161 gcgtggtttt cctacatttt cccatcctga ttatcgccgc ttatgcgttt aacactgaag
    11221 atgcggcgtt tagttttcca ccgcagggcc tgacgctgcg ctggtttagc gtggcagcac
    11281 agcgtagtga tattcttgat gccgtgacac tgtcacttaa agtggcggcg ctggcgacat
    11341 taatggcgct ggtgttaggg acgctggcag ctgccgcgct gtggcgacga gactttttcg
    11401 gcaaaaacgc catttcgctg ttactgctgc tgcccattgc gctgccgggc attgtcactg
    11461 gtctggcgct attaaccgct tttaaaacca tcaatctgga gccgggattt ttcaccatcg
    11521 tggtcggtca tgcgactttt tgcgtagtgg tggtgtttaa caatgtcatc gcccgttttc
    11581 gccgcacctc gtggagtctg gttgaggcgt caatggatct tggggccaat ggctggcaaa
    11641 ccttccgcta cgtagtgttg ccgaatctca gttcggcgct actggcagga ggaatgctgg
    11701 cgtttgcctt gtcgttcgat gaaatcatcg ttacgacttt tacggcaggt catgaacgaa
    11761 cgttaccgtt gtggttgctc aatcagcttg ggcgaccgcg tgatgtaccg gtaactaacg
    11821 tggtggcact gctggttatg ttggtaacaa ccttgccgat cctgggggcc tggtggctaa
    11881 cccgcgaagg cgacaatggt caataaccac tgatacagga atatgctatg caacataagt
    11941 tactgattaa cggagaactg gttagcggcg aaggggaaaa acagcctgtc tataatccgg
    12001 caacggggga cgttttactg gagattgccg aggcatccgc agagcaggtc gatgctgctg
    12061 tgcgcgcggc agatgcagca tttgccgaat gggggcaaac cacgccgaaa gtgcgtgcgg
    12121 aatgtctgct gaaactggct gatgttatcg aagaaaatgg tcaggttttt gccgaactgg
    12181 agtcccgtaa ttgtggcaaa ccgctgcata gtgcgttcaa tgatgaaatc ccggcgattg
    12241 tcgatgtttt tcgctttttc gcgggtgcgg cgcgctgtct gaatggtctg gcggcaggtg
    12301 aatatcttga aggtcatact tcgatgatcc gtcgcgatcc gttgggggtc gtggcttcta
    12361 tcgcaccgtg gaattatccg ctgatgatgg cagcgtggaa actggctccg gcgctggcgg
    12421 cagggaactg cgtagtgctt aaaccatcag aaattacccc gctgaccgcg ttgaagttgg
    12481 cagagctggc gaaagatatc ttcccagcag gcgtgattaa cgtactgttt ggcagaggca
    12541 aaacggtggg tgatccgctg accggtcatc ccaaagtgcg gatggtgtcg ctgacgggct
    12601 ctatcgccac cggcgagcac atcatcagcc ataccgcgtc gtccattaag cgtactcata
    12661 tggaacttgg tggcaaagcg ccagtgattg tttttgatga tgcggatatt gaagcagtgg
    12721 tcgaaggtgt acgtacattt ggctattaca atgctggaca ggattgtact gcggcttgtc
    12781 ggatctacgc gcaaaaaggc atttacgata cgctggtgga aaaactgggt gctgcggtgg
    12841 caacgttaaa atctggtgcg ccggatgacg agtctacgga gcttggacct ttaagctcgc
    12901 tggcgcatct cgaacgcgtc agcaaggcag tagaagaggc gaaagcgaca gggcacatca
    12961 aagtgatcac tggcggtgaa aagcgcaagg gtaatggcta ttactatgcg ccgacgctgc
    13021 tggctggcgc attacaggac gatgccatcg tgcaaaaaga ggtatttggt ccagtagtga
    13081 gtgttacgcc cttcgacaac gaagaacagg tggtgaactg ggcgaatgac agccagtacg
    13141 gacttgcatc ttcggtatgg acgaaagatg tgggcagggc gcatcgcgtc agcgcacggc
    13201 tgcaatatgg ttgtacctgg gtcaataccc atttcatgct ggtaagtgaa atgccgcacg
    13261 gtgggcagaa actttctggt tacggcaagg atatgtcact ttatgggctg gaggattaca
    13321 ccgtcgtccg ccacgtcatg gttaaacatt aagttagatg ttagtggctg ttataggacg
    13381 gccactatcg ttttaggttc tgagtttaat ggcggttaag tattagaatg attacattaa
    13441 taactagcgt aaaaattagg ctaaatgttt gatggcgatt tttcatcc
//
LOCUS       DAPHDW010000122        13395 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.124, whole genome shotgun sequence.
ACCESSION   DAPHDW010000122 DAPHDW010000000
VERSION     DAPHDW010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13395
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.124"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(579..1670)
                     /gene="ychF"
                     /locus_tag="RH057_003470"
     CDS             complement(579..1670)
                     /gene="ychF"
                     /locus_tag="RH057_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HDV3129787.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            complement(1787..2371)
                     /gene="pth"
                     /locus_tag="RH057_003471"
     CDS             complement(1787..2371)
                     /gene="pth"
                     /locus_tag="RH057_003471"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HDV3129788.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGGEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            2649..2927
                     /gene="ychH"
                     /locus_tag="RH057_003472"
     CDS             2649..2927
                     /gene="ychH"
                     /locus_tag="RH057_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HDV3129789.1"
                     /translation="MKRKNASLLGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            complement(2982..4661)
                     /gene="dauA"
                     /locus_tag="RH057_003473"
     CDS             complement(2982..4661)
                     /gene="dauA"
                     /locus_tag="RH057_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HDV3129790.1"
                     /translation="MNKIFSSHVMPFRALIDACWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(4786..5733)
                     /gene="prs"
                     /locus_tag="RH057_003474"
     CDS             complement(4786..5733)
                     /gene="prs"
                     /locus_tag="RH057_003474"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HDV3129791.1"
                     /translation="MPDMKLFAGNATPELAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            complement(5884..6735)
                     /gene="ispE"
                     /locus_tag="RH057_003475"
     CDS             complement(5884..6735)
                     /gene="ispE"
                     /locus_tag="RH057_003475"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HDV3129792.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            complement(6735..7358)
                     /gene="lolB"
                     /locus_tag="RH057_003476"
     CDS             complement(6735..7358)
                     /gene="lolB"
                     /locus_tag="RH057_003476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HDV3129793.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            7572..8828
                     /gene="hemA"
                     /locus_tag="RH057_003477"
     CDS             7572..8828
                     /gene="hemA"
                     /locus_tag="RH057_003477"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HDV3129794.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFADSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            8870..9952
                     /gene="prfA"
                     /locus_tag="RH057_003478"
     CDS             8870..9952
                     /gene="prfA"
                     /locus_tag="RH057_003478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HDV3129795.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            9952..10785
                     /gene="prmC"
                     /locus_tag="RH057_003479"
     CDS             9952..10785
                     /gene="prmC"
                     /locus_tag="RH057_003479"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV3129796.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            10782..11174
                     /locus_tag="RH057_003480"
     CDS             10782..11174
                     /locus_tag="RH057_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HDV3129797.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            11178..11987
                     /locus_tag="RH057_003481"
     CDS             11178..11987
                     /locus_tag="RH057_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HDV3129798.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDVWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVEQCPEDPISEMIRAQINNIAHKHIVLH"
     gene            12023..12877
                     /gene="kdsA"
                     /locus_tag="RH057_003482"
     CDS             12023..12877
                     /gene="kdsA"
                     /locus_tag="RH057_003482"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HDV3129799.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(13026..13133)
                     /locus_tag="RH057_003483"
     CDS             complement(13026..13133)
                     /locus_tag="RH057_003483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HDV3129800.1"
                     /translation="MTLAQFAMIFWHDLAAPILAGIITAAIVGWWRNRK"
BASE COUNT         3338 a         3372 c         3451 g         3234 t
ORIGIN      
        1 aatattacaa caaacagcgg ttaaaaaatt tggtgaagat tttttgtaat aatcctaatt
       61 agttatgggg ttttcgcttg atgtatctgc ataacaaaaa caatatgcgc catttttgca
      121 ttactaataa gaaaaagcaa aaaacatcga tattcatttt tggtcaatgg gttttctggc
      181 ctatcgttgt tattgtgcta ctggttatca ttttcatctt gccaggttcg cacgtaatct
      241 cattattcgg ggactttatc tgcgggatat tacccttcag gtaagtcctt ttctcgccag
      301 catattggag cgtgatcaat attgatcagc tgtgaagggg gtgatgtgta gtggaacaaa
      361 aacttacgtt atatcactac taatgagata tccgtcatct gaacactgat gtctttaatt
      421 attctggtgg ttcactgtag gcaataagca caaaagtgta ggatgttaca agaatgatta
      481 ggactcggtg aaatgaaaaa tccacgcaat tgcgtggatt ttatatactt ttgcgctctt
      541 catgagattt agcgaaacct catgagacaa caaattaatt agacgttgaa aaggaagttc
      601 atcacatcgc catctttaac gatgtaatct ttaccttctg cacgcatttt gcctgcttct
      661 ttcgcgcctt gttcaccttt gtaagtgatg aaatcttcaa acgagatggt ttgtgcacgg
      721 ataaagcctt tttcaaaatc agtatggatt ttgcccgctg cctgcggcgc ggttgctcca
      781 accggaatgg tccatgcacg cacttctttc accccagcgg tgaagtaagt ttgcaggttc
      841 agcagcttat aaccggcacg gatcacacgg ttcaggcccg gctcttccag cccaagctcc
      901 tgcataaact cgtcacgttc ttcgtcgtcc agttcggcaa tgtctgcttc aacagcagca
      961 caaaccggaa ccacaacaga accttctttc gccgcgattt cacgcacctg gtcaagatat
     1021 gggttgtttt caaaaccgtc ttcgttgacg ttggcgatgt acattgttgg tttcagcgtc
     1081 aggaagctca ggtaacgaat agccgctttc tcttcagcgc ttaaatccag cgcgcgcagc
     1141 atacctgcgt tttccagctg gggcaggcat ttttccagga ccgccagctc agctttcgcg
     1201 tctttatcgc cacctttggc tttcttctgt acgcgatgaa tcgcacgttc gcaggtgtcg
     1261 aggtctgcca gcgccagttc ggtgttgata acttcaatat cgtccgccgg gttaactttg
     1321 ccggaaacgt gaatgatgtt gtcattttca aagcagcgaa caacgtgacc gatcgcttcg
     1381 gtttcacgga tgttagtcag gaactggtta cccagacctt cgcctttcga tgcgcctttt
     1441 accagaccgg cgatatcgac aaattccatg gtcgtgggaa gcgtacgctg cggttttacg
     1501 atttcagcca gttgatccag gcgagggtca ggcattggta cgacgcctgt gttcggctca
     1561 atggtgcaga atggaaagtt ggccgcttca ataccggctt tggtcagcgc gttgaacagg
     1621 gtagatttcc cgacgttggg caaaccgacg ataccgcatt tgaatcccat gatttaaatc
     1681 accttaatat cttaataatc aacctgttat tgctaacaga ttgcagaaat ggaaataact
     1741 ttgcctatta tacacggcac tcggcaaaaa tgccgcagac aacgacttat tgcgctttaa
     1801 aggcgtgcaa tcggttcgtt gctttggtca agccatctgt aaaccacatt tcagtacaac
     1861 gcgccgcttc gtcgatggct tcatcaatta acttctgttc actaacaggc ggtttgccta
     1921 acacaaaacc gacaacttta tttttatcgc ccggatgacc gattccgatg cgtaaacggt
     1981 gaaagttagg gttattaccc aatttactga tgatgtcttt cagtccattg tgaccaccat
     2041 ggccaccgcc caacttaaat ttggcgacgc caggaggcag atccagttcg tcgtgggcca
     2101 ccagaatttc gtccggatta atgcggaaaa aactggccat cgccgcaacg gctttgccgc
     2161 tgagattcat aaatgtagtc gggactaaca ggcggacatc ttcgcctcca agggtgactc
     2221 gcgaagtata accaaagaat ttagcctctt cgcgcagcgg agcgcgcaaa cgctctgcca
     2281 gtaagtcaac gaaccaggca ccagcattat gtcgcgttgc ggcgtattca gcaccggggt
     2341 tcgccaggcc gacaatcaat ttaatcgtca cgtttttttg tcctgagtgt gtacataact
     2401 ggcgcgtagt ttactggttg cggccccgct tgacaaaaaa ctgcgtatca aatgcagata
     2461 acgtaataat tgcctgagtg gactattaga aagtcaaggt gttcaggcgt ttatttgtaa
     2521 agttttgttg aaataagggt tgtaattgtg atcacgcccg cacataaccc acagggtgtt
     2581 gtctatactt tacacataag gaagaggggt attccctgtt acaacccaga aagttccgga
     2641 ggtgacatat gaaacgcaaa aacgcttcgt tactcggtaa cgtgctcatg gggttgggtc
     2701 tggtggtaat ggtggtcggc gtggggtatt caatcctcaa ccagttacca cagtttaata
     2761 tgccccagta tttcgcacat ggtgcagtgc taagtatttt cgtcggtgcc attctctggc
     2821 tggcgggtgc ccgtgttggc gggcatgaac aggtgtgcga ccgttactgg tgggttcgcc
     2881 actatgacaa acgttgccgc cgtagcgata atcgccgtca tagctaacaa ataatgcagt
     2941 ttgctgacca gtcagtttgc gctgactggc caaactcgta cttataaatc cgccatcgcc
     3001 gcgcgacgat tcgggaagaa cgcgaggcgt cccgggatcg gttgaatgcc tgcgcgagcc
     3061 atagtgcgca gaggctggaa ttccacgttg cacacgcgca gttcacatcc ttcgggcaga
     3121 cgcttcacaa aacgctggaa cgcatcaaga ccaccagcat caagtaccgg aacggcatcc
     3181 cacttcagaa tcacaatccg tttgccttca agacgtgact ccaggtccgt gaataagcct
     3241 tcagcagcag caaaaaacag cgggccaata acgcgcagaa ccaggacatc gtctggaaca
     3301 tctacgacta ccggtgccag gcgagtcata cgtgcgatac gacgcataaa cagcagcgat
     3361 gccagcacga tccccacgct gatggcaata accatatcaa acagcacggt cagcgacatg
     3421 cacagcagca tgacgatgat gtcatctttc ggcgcatgac gcagcaagtc gaccacttta
     3481 tgcgcttcac tcatgttcca cgccaccatc aacagcaggg ctgccatagc ggaaagcggc
     3541 agccaggaga gcagcggtgc cagtaccagc agggcaagaa taaccagaat agagtggatc
     3601 accgccgaga taggggacgt tgccccggca cggacgttag cggcagaacg cgcgatggca
     3661 gctgtagcgg taataccacc aaagaacgga gcgatgatat tccccagtcc ttgtccaacc
     3721 agttcgctat tcgccttgtg tttcgtcccg gtcataccat ccagcaccac ggcgcagagc
     3781 agagattcga ttgcgccgag cattgccatt gagaatgccg caggcagcag tgtgcgaata
     3841 gaatcccagg ttagcgtgaa ttctgaatta ggcagatccc acggcagcac cagttgcggc
     3901 agcagttgcg gaataccgtt accctgagaa ccatcggcca ggacgtagtg gaattgcgaa
     3961 ccgatggtag caacatgtcc gccgagcagg ttaacaatcc ccatcaccgc gcaaccagcc
     4021 agcaaggccg gaaggtgacc gggtaaacga atgcccagac gcggccagaa aacaagaata
     4081 cctagcgtca caatgccaat tgcggcatcg cccacgttaa tggtcggcag cgccataaat
     4141 aatgcgccga ctttttgtag ataatgttcc gggacatggg ccatttgcag accgagaaaa
     4201 tctttaatct gcatggtacc gatggtgatc ccgatacccg atgtgaaacc taaggtgacg
     4261 gaaaccggaa tatactcaat caggcgacca aagcgtgcca gacccatcag aatcaaaaag
     4321 atccccgaca gcaaggtcgc aaccagcagt cctgccagtc caaactgttg cgacacggga
     4381 tagagaatta ccacaaatgc cgcagtcgga ccggaaacgc taaagcgtga tccacccgtc
     4441 agagcaatga caatccccgc aacagctgca gtatataaac cgtactgggg tgccacacca
     4501 ctaccaatag ccaacgccat cgccagcggg atagcaataa tcccgacggt tatcccggca
     4561 atcaggtcac gggtaaaccg tgcggcagta tatttttctt tccagcaagc gtcgatcaga
     4621 gcgcggaaag gcatcacatg tgaggaaaat attttgttca caataatgtt tcatccgtga
     4681 gcgcatcatc tgtcaactaa atggcaggtg aaggaggcat aggtcataca aatggatatt
     4741 acagacaaaa aaacccgccg cagcgggtct ttgagccggg ttcgattagt gttcgaacat
     4801 ggcagagatc gattcttcgt tgctgatacg acgaatcgct tcggccagca tacctgacag
     4861 ggtcagagta cgcacgttcg gcagtgattt gatttcatcg ctcagcggaa tggtatcgca
     4921 gacaacgact tcatcaatta cagagttacg caggttgttc gccgcgttgc cagagaagat
     4981 cgggtgagtc gcgtacgcaa atacacgttt agcaccacgt tctttcagcg cttcagcagc
     5041 tttacacagc gtaccgccag tgtcgatcat atcatcgacc agtacgcagt cacgacctgc
     5101 aacgtcaccg atgatatgca tcacctggga aacgttcgca cgcggacgac gtttgtcgat
     5161 gattgccata tcggtatcgt tcagcagctt agcgatagcg cgggcacgca caacgccgcc
     5221 gatgtccgga gaaaccacaa ttgggttatc cagattcagc tgcagcatgt cttccagcag
     5281 gatcgggcta ccaaatacgt tatcaaccgg aacgtcgaag aaaccctgaa tctgttcagc
     5341 gtgcagatcc actgtcagca cacggtcaac accgacgctg gagaggaagt ctgcaaccac
     5401 tttcgcagtg attggtacac gagcggaacg gacgcgacgg tcctggcgcg catagccaaa
     5461 gtaggggata acagcggtga tacgacctgc ggaagcacga cgcagggcat caaccataac
     5521 gactaattcc atcaggttgt cgttagtagg ggcacaagtg gactggatga tgaaaatatc
     5581 accaccgcgt acattttcat taatttgtac gctgacttcg ccatcgctaa agcgacctac
     5641 agcggcgtcg ccgagtgaag tgtacaggcg gttggcaata cgttgtgcta gttccggggt
     5701 ggcgttacca gcaaaaagct tcatatcagg cacgagaaga acctcaggca tgcgtccatt
     5761 ggtggaaaga atctgccgaa aactgtgcgg gccaggcatg atcctttcca ggcggtgtat
     5821 taaagagcgc gatgcaacgt ctggaacaag gtgacgttgt caccgaaact cagcttgccc
     5881 ggcttaaagc atggctctgt gcaatgggga aagattaacg cctttcgcca caaagccatt
     5941 gagccattcc ggggcttgct ctagcacctg gcgggcttca gactctgtat caaattcagc
     6001 aaagacacag gcccctgtcc cagtcaggcg cgacggggcg tattctaaca gccaggaaag
     6061 caccgcatca acctcgcgaa aacgttttct tgcgataacc tcgcaatcat tgctgaattc
     6121 acattttagc aacgtttcta ttgacctttt tggcgtattg cgcgggagtt caggatcttt
     6181 aaaaatcacc ggagtcggaa tacttacacc agggtgcgcc accagatacc acttctctgg
     6241 cggatccacc ggcgttagta tttcaccaac gccttcggca aacgcggcat gcccccgaac
     6301 aaagacagga acatctgcgc ccagcgtcag ccccatttcc gccagctcat ccatgcttag
     6361 cccgcattgc cagagatgat ttaatgccac caggaccgtc gcggcattgg atgaaccacc
     6421 gccgagaccg ccgcccatcg gcaaacgctt gtcaatgctg atattcgcac cgcttcccgt
     6481 cggaagacgc ccgctgtctg ccgcagtttt catcaacaac cgcgctgcgc gaacgatcag
     6541 gttatcttca tgttccacgc cttcaacggg cgttaacaga cgaatatccc catcgtcacg
     6601 aagctcaatg ctgatggtgt cgccgtaatc aagaaactga aacagcgttt gcagcgtgtg
     6661 gtaaccatcc gcacgctgac cggtaatgta taaaaacaga ttaagttttg ccggagaggg
     6721 ccactgtgtc cgcattattt cactatccag ttatccattt ttaacttgat gcgttgacca
     6781 ccgtcggtga gttccatatt ggctggcatc gcaggttgcg ttttggtgtc ataaccacca
     6841 taaacaacct tccagttttt gccattctgg ctgtaggtaa tttcgctcag gcggtactgg
     6901 tcgtccagtt tgtagtcggt tgcatcaccc ggtaaaccta aaatccactg gcgcaagctg
     6961 ttgagcggaa ttggcattcc ggtcaatttg ccaatcatct cttcggcgtc atcggcggta
     7021 taacgctgac ctttattgtc gactaactgc acgttacccg gttgagcatt cagctccagt
     7081 tccgtgctgc ccaatgggtt agtgagcagc agacggtagc gatcctggcc ggtttgctgc
     7141 cagaaaaagc gggcgtacac tttttgttgg tcagaaatat aagcgaacgc gccgcgagtc
     7201 tgatactgat taagattgcg cacgtcttgc tgatgctgac gccattgtgg cgaatccggg
     7261 cttttgccag gacctttggg cgtggtaacg gaacaggcag tgagcacaag agcagccagc
     7321 ggtagcaggc ggataagacg aaaatcgggc aggggcatag tgatgacaag tccttgagat
     7381 acgttgcagt tataaccctt aatgctagcg ttaccgtccg ctatcgtcta tgttcaagtt
     7441 gtcttaattg ccagaatcta acggctttcg gcaattactc caaaaggggg cgctctcttt
     7501 tattgatctt acgcatcctg tatgatgcaa gcagactaac cctatcaacg ttggtattat
     7561 ttcccgcaga catgaccctt ttagcactcg gtatcaacca taaaacggca cctgtatcgc
     7621 tgcgagaacg tgtatcgttt tcgccggata agctcgatca ggcgcttgac agcctgcttg
     7681 cgcagccgat ggtgcagggc ggcgtggtgc tgtcgacgtg caaccgcacg gaactttatc
     7741 ttagcgttga agagcaggat aacctgcaag aggcgttaat ccgctggctt tgcgattatc
     7801 acaatcttaa tgaagaagat ctgcgtaaaa gcctctactg gcatcaggat aacgacgcgg
     7861 ttagccattt aatgcgtgtt gccagcggcc tggattcact ggttcttggg gagccgcaga
     7921 tcctcggtca ggttaaaaaa gcgtttgccg attcgcaaaa aggtcatatg aaggccagcg
     7981 aactggaacg catgttccag aaatctttct ctgtagcgaa acgcgttcgc actgaaacag
     8041 atatcggtgc cagtgctgtg tctgtcgctt ttgcggcttg tacgctggcg cggcagatct
     8101 ttgaatcgct ctctacggtc acagtgttgc tggtaggcgc gggcgaaacc atcgagctgg
     8161 tagcgcgtca tctgcgcgaa cataaagtac agaagatgat tatcgccaac cgcactcgcg
     8221 aacgtgccca aattctggca gatgaagttg gcgcggaagt gattgccctg agtgagatcg
     8281 acgaacgtct gcgcgaagcc gatatcatca tcagttccac cgccagcccg ttaccgatta
     8341 tcgggaaagg catggtggag cgcgcattaa aaagtcgtcg taaccaacca atgctgttgg
     8401 tggatattgc cgttccgcgc gatgttgagc cggaagttgg caaactggcg aatgcttatc
     8461 tttatagtgt ggacgatctg caaagcataa tttcgcacaa cctggcgcag cgtaaagccg
     8521 cagcggttga ggcggaaact attgtcgctc aggaaaccag cgaatttatg gcttggctgc
     8581 gagcacaaag cgccagcgaa accattcgcg agtatcgcag ccaggcagag cacgttcgcg
     8641 atgagttaac cgccaaagcg ttagcggccc ttgagcaggg cggcgacgcg caagccatta
     8701 tgcaggatct ggcatggaaa ctgactaacc gcttgatcca tgcgccaacg aaatcacttc
     8761 aacaggccgc ccgtgacggg gataacgaac gcctgaatat tctgcgcgac agcctcgggc
     8821 tggagtagca gtacatcatt ttcttttttt acagggtgca tttacgccta tgaagccttc
     8881 tatcgttgcc aaactggaag ccctgcatga acgccatgaa gaagttcagg cgttgctggg
     8941 tgacgcgcaa actatcgccg accaggaacg ttttcgcgca ttatcacgcg aatatgcgca
     9001 gttaagtgat gtttcgcgct gttttaccga ctggcaacag gttcaggaag atatcgaaac
     9061 cgcacagatg atgctcgatg atcctgaaat gcgtgagatg gcgcaggatg aactgcgcga
     9121 agctaaagaa aaaagcgagc aactggaaca gcaattacag gttctgttac tgccaaaaga
     9181 tcctgatgac gaacgtaacg ccttcctcga agtccgagcc ggaaccggcg gcgacgaagc
     9241 ggcgctgttc gcaggcgatc tgttccgtat gtacagccgt tacgccgaag cccgccgctg
     9301 gcgggtagaa atcatgagcg ccagcgaggg tgaacatggt ggttataaag agatcatcgc
     9361 caaaattagc ggtgatggtg tgtatggtcg tctgaaattt gaatctggcg gtcatcgcgt
     9421 gcagcgtgtt cctgctacgg aatcgcaggg tcgtattcat acttctgctt gtaccgttgc
     9481 ggtaatgcca gaactgcctg acgcagaact gccggacatc aacccagcag atttacgcat
     9541 tgatactttc cgctcgtcag gggcgggtgg tcagcacgtt aacaccaccg attcggcaat
     9601 tcgtattact cacttgccga ccgggattgt tgttgaatgt caggatgaac gttcacaaca
     9661 taaaaacaaa gcgaaggcgc tttcagtgct cggtgctcgc atccacgctg ctgaaatggc
     9721 aaaacgccaa caggccgaag catctacccg tcgtaacctg ctggggagtg gcgatcgcag
     9781 cgaccgtaac cgtacttaca atttcccgca ggggcgcgtt accgatcacc gcatcaacct
     9841 gacgctctac cgcctggatg aagtgatgga aggtaagctg gatatgctga ttgaaccgat
     9901 tatccaggaa catcaggccg accaactggc ggcgttgtcc gagcaggaat aatggaatat
     9961 caacactggt tacgtgaagc aataagccaa cttcaggcga gcgaaagccc gcggcgtgat
    10021 gctgaaatcc tgctggagca tgttaccggc agagggcgta cttttattct cgcctttggt
    10081 gaaacgcagc tgactgacga acaatgtcag caacttgatg cgctactgac acgtcgtcgc
    10141 gatggtgaac ccattgctca tttaaccggg gtgcgagaat tctggtcgtt gccgttattt
    10201 gtttcgccag cgaccttaat tccgcgcccg gatacggagt gtctggtgga gcaggcactg
    10261 gcgcggttgc ctgaacaacc ttgccgtatt cttgatctcg ggacgggtac cggggcgatt
    10321 gcgcttgcgc tggctagcga gcgcccggac tgcgaaatta tcgctgtaga tcgtatgcct
    10381 gatgctgtct ccctggcaca acgtaatgcc cagcatctgg cgatcaaaaa tatccacatt
    10441 ctgcaaagcg actggtttag cgcgctagcc gggcagcagt ttgcgatgat tgtcagcaat
    10501 ccgccgtata ttgacgagca ggacccacat cttcaacaag gcgatgtccg ctttgagccg
    10561 ctcactgcgc tggttgcggc agacagtgga atggcagaca tcgtgcatat catcgaacag
    10621 tcgcgtaacg cgctggtatc cggcggcttt ctgcttctgg aacatggctg gcagcagggc
    10681 gaagcggtgc gacaagcatt tatcctcgcg gggtatcatg acgtcgaaac ctgccgtgac
    10741 tatggtgata acgagcgcgt aacgctcggc cgctattatc aatgacaagt ttttctacac
    10801 tgcttagtgt tcatcttatt agtatcgcgc tttctgttgg gctattaacc ttacgtttct
    10861 ggctacgtta tcagaagcat ccacaggcat ttgctcgctg gacgcgcatt gtgccgccgg
    10921 ttgtcgatac ggtgttattg ttaagcggca ttgcgttgat ggctaaagcg cacatcctgc
    10981 cattttccgg gcaggcacag tggctgactg aaaagctgtt tggagttatc atttatatcg
    11041 ttttgggttt tattgcactc gattatcgtc gtatgcacag tcagcaggcg cgcattattg
    11101 ccttcccgct ggcgttggtg gtgctgtaca tcatcattaa actcgccacc acaaaagtac
    11161 cgttactggg gtaagtcatg agatcgttag ctgatttcga atttaataaa gcgccattgt
    11221 gcgaaggcat gatcctggct tgcgaagcaa tccgccgcga ttttccctcg caagatgttt
    11281 acgacgaact ggagcgtctc gttagtctgg cgaaggaaga aatcagccag cttctgcctt
    11341 tagaagagca gctggaaaaa ctgatcgcgc tgttttacgg cgactgggga tttaaagcct
    11401 cacgcggcgt ttatcgtctt tccgatgcat tatggctgga ccaggtgtta aagaatcgac
    11461 agggcagtgc ggtatcatta ggtgcggttt tattatgggt cgcgaatcgt ctcgatttgc
    11521 cgctgctgcc ggtgattttc cctacgcagc tgatattgcg cattgaatgt ccggatggcg
    11581 aaatttggct gattaatcct tttaacggtg aatcgttaag cgaacatatg ctggacgtat
    11641 ggttaaaggg aaatatcagc ccgtcggcgg aactgtttta tgaagacctt gatgaagctg
    11701 ataacattga ggtaatccgc aaattgctgg atacactcaa agcctcgttg atggaagaaa
    11761 atcagatgga gctggcgtta cgcaccagcg aagctttatt acaattcaac cctgaagatc
    11821 cctatgaaat tcgcgatcgc gggttgattt atgcgcaact ggattgcgaa cacgttgcgt
    11881 tgaacgattt aagttatttc gttgaacagt gtccggaaga cccgatcagc gaaatgatcc
    11941 gtgcgcaaat aaataacatc gcgcataaac atattgtgct gcattaatta atcgacattt
    12001 tactcaagat taaggcgatc ctatgaaaca aaaagtggtt agcattggcg acatcaacgt
    12061 agcaaatgac ctgccgttcg tactgtttgg cggtatgaac gtgctggaat ctcgcgatct
    12121 ggcgatgcgc atttgcgagc actacgtaac tgtgacccag aaactgggta tcccttacgt
    12181 gttcaaagcc tcttttgaca aagccaaccg ctcctccatc cactcttatc gtggaccggg
    12241 cctggaagaa gggatgaaaa tcttccagga gttgaagcag acttttggcg tgaaaattat
    12301 caccgacgtt cacgaaccaa gtcaggcaca gcccgttgct gatgtcgtgg atgtgattca
    12361 gttgccggcg tttcttgctc gccagactga cctggttgaa gccatggcga aaaccggtgc
    12421 ggtaattaac gtcaagaaac cacagtttgt cagcccggga cagatgggta atatcgttga
    12481 taaattcaaa gaaggcggca acgaaaaagt gattctttgc gatcgtggtg ctaacttcgg
    12541 ctatgacaac ctggttgtcg atatgctggg cttcagcatt atgaagaaag tgtctggtaa
    12601 ctcgccggtg attttcgacg tgacccacgc actgcaatgc cgcgatccgt ttggcgcagc
    12661 ttccggtggt cgtcgtgctc aggtggctga gctggcacga gccggtatgg cggtaggtct
    12721 ggcggggctg tttattgaag cgcatccgga tccggaacat gcgaaatgtg atggtccatc
    12781 cgcgctgccg ctggctaaac tggaaccgtt cctcaagcag atgaaagcga ttgatgatct
    12841 ggtgaaaggt ttcgaagaac tggataccag caagtaatct tttttgcttg aaaaataaag
    12901 tcttagcgtt ctgcgttaag acttttttca tgggtgccag atacaaaaaa ggccgcaggc
    12961 tgttacccct gcggccggtt tcgggcgcat attgccatca cggcagcctg acgcccgttt
    13021 tcaccttact tccggttacg ccaccagccg acaatcgctg cggtaataat tcccgccagg
    13081 atcggtgctg ccaggtcgtg ccagaaaatc atggcaaact gcgcgagcgt catatagccg
    13141 ccttgttgta atgacaacat tttgcggcta ttcttgaatt gttctggttc aagattagcc
    13201 cccgttctgt tgtcaggtta aacctctcaa cgtgcggggg ttttctcttt ccagcaacca
    13261 atgccaccag ggataaagcc cccgcaacat tgcgcctcac cggataacgc cggcttgatg
    13321 tggatactac gtctcaattc atcttcactt catccctgaa atgtttgcaa tgaagagtgc
    13381 attccggttt ttcaa
//
LOCUS       DAPHDW010000123        13361 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.125, whole genome shotgun sequence.
ACCESSION   DAPHDW010000123 DAPHDW010000000
VERSION     DAPHDW010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13361
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.125"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            42..872
                     /locus_tag="RH057_003484"
     CDS             42..872
                     /locus_tag="RH057_003484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HDV3129801.1"
                     /translation="MSMPLSNAANNQFYINNHFLHHPKVDNELTRKYERARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLDISKESWPFNYVTEACRESDGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSLFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPVSQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIGMGN
                     DPIIGIEAYPGTAEIHAKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1013..1786)
                     /gene="uxuR"
                     /locus_tag="RH057_003485"
     CDS             complement(1013..1786)
                     /gene="uxuR"
                     /locus_tag="RH057_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HDV3129802.1"
                     /translation="MKSATSAQRPYQEVGAMIRDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2001..3461)
                     /locus_tag="RH057_003486"
     CDS             complement(2001..3461)
                     /locus_tag="RH057_003486"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HDV3129803.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRVLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GAQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMRYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTAAYQQLCERGARECVAAL"
     gene            complement(3542..4726)
                     /gene="uxuA"
                     /locus_tag="RH057_003487"
     CDS             complement(3542..4726)
                     /gene="uxuA"
                     /locus_tag="RH057_003487"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HDV3129804.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAV
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYEVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     SR"
     gene            5066..6409
                     /gene="gntP"
                     /locus_tag="RH057_003488"
     CDS             5066..6409
                     /gene="gntP"
                     /locus_tag="RH057_003488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HDV3129805.1"
                     /translation="MHVLNILWVVFGIGLMLVLNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISVVA"
     gene            complement(6659..7561)
                     /gene="fimH"
                     /locus_tag="RH057_003489"
     CDS             complement(6659..7561)
                     /gene="fimH"
                     /locus_tag="RH057_003489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HDV3129806.1"
                     /translation="MKRVITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPAVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7581..8084)
                     /gene="fimG"
                     /locus_tag="RH057_003490"
     CDS             complement(7581..8084)
                     /gene="fimG"
                     /locus_tag="RH057_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HDV3129807.1"
                     /translation="MKWCKRGYVLAAILALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8097..8627)
                     /gene="fimF"
                     /locus_tag="RH057_003491"
     CDS             complement(8097..8627)
                     /gene="fimF"
                     /locus_tag="RH057_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HDV3129808.1"
                     /translation="MRNKPFYLLCAFLWLAVSHALAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAASGLGIQLLNEQQNQIPLNAPSSALSWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8637..11273)
                     /gene="fimD"
                     /locus_tag="RH057_003492"
     CDS             complement(8637..11273)
                     /gene="fimD"
                     /locus_tag="RH057_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HDV3129809.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     AELYFNPRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVSGMNLLADDACVPLTSIIHDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRIGGNSHYAYLNLQSGLNIG
                     AWRLRDNTTWSYNSSDRSSGSKNKWQHINTWLERDIIPLRSRLTLGDGYTQGDIFDGI
                     NFRGAQLASDDNMLPDSQRGFAPVIHGIARGTAQVTIKQNGYDIYNSTVPPGPFTIND
                     IYAAGNSGDLQVTIKEADGSTQIFTVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEK
                     PRFFQSTLLHGLPAGWTIYGGTQLADRYRAFNFGIGKNMGALGALSVDMTQANSTLPD
                     DSQHDGQSVRFLYNKSLNESGTNIQLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGV
                     IQVKPKFTDYYNLAYNKRGKLQLTVTQQLGRSSTLYLSGSHQTYWGTSNVDEQFQAGL
                     NTAFEDINWTLSYSLTKNAWQKGRDQMLALNVNIPFSHWLRSDSKSQWRHASASYSMS
                     HDLNGRMTNLAGVYGTLLEDNNLSYSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANI
                     GYSHSDDIKQLYYGVSGGVLAHANGVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTD
                     WRGYAVLPYATEYRENRVALDTNTLADNVDLDNAVANVVPTRGAIVRAEFKARVGIKL
                     LMTLTHNNKPLPFGAMVTSESSQSSGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCV
                     ANYQLPPESQQQLLTQLSAECR"
     gene            complement(11340..12065)
                     /gene="fimC"
                     /locus_tag="RH057_003493"
     CDS             complement(11340..12065)
                     /gene="fimC"
                     /locus_tag="RH057_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313302.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HDV3129810.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENTLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGARVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(12102..12641)
                     /locus_tag="RH057_003494"
     CDS             complement(12102..12641)
                     /locus_tag="RH057_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3129811.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMKFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAVGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIATNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(12706..13254)
                     /gene="fimA"
                     /locus_tag="RH057_003495"
     CDS             complement(12706..13254)
                     /gene="fimA"
                     /locus_tag="RH057_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HDV3129812.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTVHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLAQEGATSSAVGFNIQLNDCDTTVADKAAIAFLGTAID
                     GTHPNVLALQSSAAGSATKVGVQILDRTGAALALDGATFSAQTTLNNGTNTIPFQARY
                     FATGAATPGAANADATFKVQYQ"
BASE COUNT         3240 a         3462 c         3237 g         3422 t
ORIGIN      
        1 ttttctaaac atcagtgaaa tactcataaa aggtaactat tatgtctatg ccattaagca
       61 atgccgctaa taaccaattc tatataaata atcacttcct tcatcatcca aaagtcgaca
      121 atgagttaac acgtaaatat gaacgagctc gcctggatac ggaaaatatc tatcttctgc
      181 ctcttgctcg tggcaataac cataattacg atggaaaaag cgttgtcgaa attagaaaac
      241 ttgatattag taaagagtcc tggccattta attatgtgac agaagcatgc agagaatccg
      301 atggtattac aacaacagga cgtatgctct atcgaaactt gaaaatcacc tcagctttag
      361 acgaaatata tggtggtata tgtaaaaagg cgcatgctgc tacagaatta gcagaaggtt
      421 tacggctaaa tttatttatg aagagccttt tcgatcctgt tgaggactat acagttcatg
      481 aaataacatt aggccctggt tgcaatgttc caggttacgc cggaaccacg ataggatata
      541 tttcaaccct tccagtttct caggcaaaaa gatggaccaa tgagcagcca agaattgata
      601 tctatattga tcagataata actgtctcag gggtagcaaa ttcttccgga ttcgcccttg
      661 ccgcattact taatgctaat attggaatgg gaaatgaccc aataattggt atagaagcct
      721 atcccggtac cgcggagatc catgcaaaaa tgggatataa cgttatcccc ggtgatgaag
      781 atgcgccact caaacgcatg actctgcaac cgtcatctct tccagaactg tttgaattaa
      841 aaaatggtga atggaattat attggtaaat agccactgag aagaatgagt actaatattc
      901 aggttataca gatatttaaa caataccctg caatttacaa agtaatactc attgcgactt
      961 cttaatttaa gaaagcgcaa ggaacgttta cccttgcgct tattataata agtcaggcgt
     1021 cgactttatc cagcggccat gaatcaaaca gatagccgtc aaaataaata tcgtcaacgt
     1081 tcgagaattc cagcagacgt tgcttaacgt tttccagatg ctgccacatt gccagcttcg
     1141 ccgctcgggc atcttttttg attaacgcgg cgaggatctg tttgtgatcg cccaaccact
     1201 ctttgcgata caggctgtca tccagatggc tgtgcaactg gatccacatt gggttgtttt
     1261 cccgccactg ccaggactga cggaacagct ccaccagcat gctgttatgc gttgcttcgg
     1321 caatagcgag atggaactgc atgtcaccgc tttcgctgct gcccggcgca ctggaagcca
     1381 gttcacgctc ttccagttgc aatgcctgac gcattttgac gatatcttcg cgggtagcct
     1441 gcagggcggc aaactcggcg atgttacttt ccagtaactg acgcgcctgt aacagctcaa
     1501 aaggacccgc atcattgcag acgttggcat ccggactgtc tgtgttgtgg ctgcctgagc
     1561 tatcaagaac atagataccg gcaccccggc gcacttccac cagccctttg atctccagca
     1621 tgatcagcgc ttcacgtacc accgtccgcg tgacatcaag catttctgca atttcacgct
     1681 ccggcggcag ccgttcgcca gggttgtacg gcgtctttat gatcagatcg cggatcatcg
     1741 ccccgacttc ctggtaaggt ctttgcgcag aggtggcaga tttcatggtg ttgtgtccgt
     1801 taggtgcggt taatcaaatt tttgccggat gcggcgtgaa cgccttatcc ggcctacaac
     1861 gggcaatttt acggcccccg ttaatctcaa actataccat tagcactgga agttggcacg
     1921 tacaggtggg caaaaaaacg aaaactggtc aaccaaatac cagtttgatt gaccagtaag
     1981 tctgtagggt aatcagttag ttacagcgca gccacacact cgcgcgcacc gcgttcgcac
     2041 agctgctgat atgccgcagt caccgcgcca acaaagtcgg cattctgcgg cagatcatcg
     2101 gcaaaaatac cgctcaggcc cagcagcgct ttcacgcggt ctgcgccctg atactgcgcg
     2161 ttaatcttct ggaactcggc cagcatcggg tcgaccacgt caatggcatt accctgctca
     2221 tccacgccct gggtgtaacg catccagcca gccacgccca gcgccaggtg acgccagctg
     2281 ccgccgtttt gcaggtgcag acgcaccggg tccagcagac gctgcggtaa cttctggctg
     2341 ccgtccattg caatctgcca ggtacggtga cgcagagatg ggttgctgaa acgctcgatc
     2401 agcagcgtcg cataggcgtt caggtccgta ccttccggca tcgacagcgt tggcgcttgt
     2461 tcctgcatca tcagggcaaa ggccgctttg cgataatccg ggttagtcat ggtgtcggca
     2521 atagtttcat agccgccgag gtaaccgagg tacgccagga aagagtggct gccgttcagc
     2581 atacgcagct tcatcatttc gaacggcaca acgtctgcaa cgaactgtgc gcccacttta
     2641 tcccagtccg ggcgaccatt aacgaagtta tcttcaatca cccactgacg gaacggttcg
     2701 caggcaatgg cgcacgggtc gtaaacaccc agctggtcag caatttcctg taaggtttct
     2761 ggcgtcgccg ctggaacgat gcggtcaacc atggtgcacg ggaaggtgac attttcttca
     2821 atccatgccg ccagctgcgg gtcacgcgcc tgagccagcc ccagtaccgc gaccttcgcc
     2881 acatgaccgt tttcacgcac gttatcgcag gacatcaccg taaacgcttt caaccctttt
     2941 tcacgacgca gacgcaaggc ttcgacgatg taaccaatcg cggacttcgg cgcagtcggg
     3001 ttttctagat cgtgcttgat cagcgggtta ttgagatcca gttgaccgct tgccgcatca
     3061 gcgcagtagc ctttttccgt gaccgttaga gagacaatcg ccgtttgcgg acgcgccatc
     3121 gcgttgagaa taccttcgca gccgtcgatt tccggatgca gcgcttcttt catcgaaccg
     3181 ataattttca gctcggtgct ttctgcgcct ttttccgcta cggtgtacag cagttgctgt
     3241 tttttcaggt tttcgatcag cacgcggtcg ttgcctggca tcaggttaac ttcgcagatg
     3301 ccccagtcgc tgtcggtgct ttccagcaga tgatgggtat acagcgcctg gtgcgcgcgg
     3361 tgaaacgccc cgcaaccgag atgcacaatg cgtgattcca gacgagaatg atcccatgac
     3421 gggcgggcaa ccggcagatt gctgtcaaca atagtagtca tcgtaaactc cagaatccgt
     3481 cttcagagag tgacggcaag gaagaaggaa ccggcacggc gcgcagccat gccggtggat
     3541 attaacggct aaagaaagcg cgctggatcg ccagttcgac accgcgaact tcggccaggc
     3601 ctttcagacg accaattgcg gagtaacctg ggttggtttt cttcttcagg tcgtccagca
     3661 tctgatgacc gtggtccgga cgcatcggga tcaggtcttc tttgccttcc gctttacgac
     3721 ggtgttcttc ttcaacaatc gctttcacca cttcgtacat atcaacgtca ccgttcaggt
     3781 gcgccgcttc gtggaaggtt ttcgggttat cttcacgcat ggtggagcgc agatgggtga
     3841 agtaaatacg cggaccgaac tgcttgatca tatcaaccag atcgttgtca gcacgcacgc
     3901 cgtaggaacc ggtgcacatg gtgaaaccgt tcgccatgct gtttacggta tcaaccatcc
     3961 actgcatatc ttcaatggtg gaaacaatgc gcggcaggcc gaggatcggg cgcggcggat
     4021 cgtccgggtg aaccgccata cgcacgccaa cttcttcagc aactggaata atcgctttca
     4081 ggaagacggc aaagttttcg cgcagcttcg ctttatcgat atctttgtac agctccaggt
     4141 gcttacggaa ctggtcgagg gtataccctt cttccgcgcc cggcagaccg gcaatgatgt
     4201 tacgcgtcag acgcgctttg tcttcatcgc tcatagtggc gaagcgaaca gcagcctgag
     4261 caatttcttc ttcggtgtaa tccgcttccg cgcctggacg tttcaggata tgcatttcga
     4321 atgcagcgaa ttcgatctgg tcgaagcgca gagctttgga gccgtctggc agcacgtatt
     4381 cgaggtcagt acgggtccag tcgagcaccg gcatgaagtt gtagcacacg gtgcgaatgc
     4441 cgcactgcgc caggttgcgc agagtctgct gatagttagc gatccactgc tcatagttgc
     4501 cagtgtgggt tttgatatct tcgtgaattg gtacgctttc tacgacagac cacaccaggc
     4561 ctgcgtcttc aacgatcgcc ttgcgtttga ggatctcttc tacggaccat acttcgccgt
     4621 tcgggatatg gtgcagcgcg gtaaccacgc cagttgcgcc cgcctgacgg acatcagcta
     4681 aagaaaccgg atcgtttggg ccgtaccagc gccaggtctg ttccatgatt tttcctcttt
     4741 cacatagtgg gttccacagc aaagagacgc ggaaccctgg agttgtttag gtatccggat
     4801 gaacaaccag accgcagccg tggcgttgca tcggatgaga tggcgtataa gttctacctt
     4861 ttggtaagac atgtcaaccc gaattctgaa attggttaac cacatcacaa gaatttcact
     4921 ctcctggttg ttgctgcaaa ttgcagcaaa aggttatcga tggttattga tcagaggatg
     4981 tgacattcat cgcaacaatg gttgaccaat ttacataaca tgtcgcccaa aataacaacg
     5041 gttcaaccag gtaaggtaaa acgttatgca tgtgcttaac attctctggg tagtattcgg
     5101 tatcggcctg atgctggtac tgaatttgaa gttcaaaatc aattcaatgg tggctttgtt
     5161 ggtggcggcg ctgtccgtcg ggatgctggc gggcatggat ttgatgtcgc tgctgcacac
     5221 tatgaaagcg ggcttcggca acacgctggg ggaactggct atcatcgtgg tgttcggtgc
     5281 ggtcatcggt aaattgatgg tcgactccgg cgcggctcac cagatagcgc atacgctgct
     5341 ggcgcgtctc ggtctgcgct atgtacagct gtcggtgatt atcatcggct tgattttcgg
     5401 tctggcgatg ttctatgaag tggcctttat catgttagcg ccgctggtta ttgttattgc
     5461 cgccgaagct aaaattccgt tcctgaaact ggcgatcccg gcagtagcag ctgccactac
     5521 cgcacattca ctgttcccac cgcagccggg tccggtggcg ctggtgaatg cttatggcgc
     5581 ggatatgggg atggtttata tctatggcgt actggtgacg atcccaagtg taatctgcgc
     5641 aggtctgatc ctgccgaagt tcctcggcaa tcttgagcgc ccaacgccat cattcctgaa
     5701 agcagatcaa ccggtagata tgaataatct gccctctttc ggcgtttcga ttctggtgcc
     5761 gctgatcccg gcgatcatta tgatctctac caccatcgcc aatatctggc tggtaaaaga
     5821 tacccctgcc tgggaagtgg ttaactttat cggttcctcg ccgattgcaa tgtttattgc
     5881 gatggtggtt gcattcgtac tctttggcac cgcacgtggt catgacatgc agtgggtgat
     5941 gaacgctttt gaaagcgcgg tgaagagtat tgcaatggtg attctgatca tcggtgcggg
     6001 tggcgtgctg aagcagacca tcatcgacac cggcattggc gacaccatcg gcatgttgat
     6061 gtcccacggc aatatctcgc cctacatcat ggcatggctg atcactgtgc taattcgtct
     6121 ggcgacgggt cagggtgtcg tttcggcgat gaccgccgcc gggattatca gtgctgcaat
     6181 cctagatcca gcaactggtc agctggttgg cgtgaatccg gcgctgctgg tgctggcgac
     6241 ggctgcgggt tccaacaccc tcacccacat taacgatgca tctttctggt tgttcaaagg
     6301 ttactttgac ctgtcagtaa aagacacgtt gaaaacctgg ggtctgctgg agctggtcaa
     6361 ctccgtggtt gggctgatta ttgtgctgat tattagcgtg gtagcgtaaa agaacaccag
     6421 agcctgccat tggcaggctc tgactgatga caaagtctaa aaatgtgccc ggccagtccc
     6481 ctcgcccctc cggggagagg gttagggtga ggggaacaga ccagcaaagt gcgctttgtt
     6541 tatgccagat gcgacgttga cgcgtcttat ctggcctaca aagggctaac gtgcaggttt
     6601 tgtaggtctg atagcgtagc gcctcaggta ccagcattag caatgccctg tgatttcttt
     6661 attgataaac aaaagtcacg ccaataatcg attgcacatt ccctgcagtc acctgccctc
     6721 cggtacgtgc ataatttgcc gttaatccca gacttaccgc cgaagtccct actgctccta
     6781 acgataccgt gttattcgct ggaataatcg taccgttgcg cgtcaactgt acgccgacgc
     6841 cctgtgcagg tgaaaacgac gcggtattgg tgaaaatcga gttgcccgca tctgcggttg
     6901 tgccggagag gtaatacccc aggttttggc ttttcgcaca ataaacggta agaggaatcg
     6961 gcactgaacc agggtagtcc ggcagagtaa cggtgacatc acgagcagaa acatcacagc
     7021 cgccagtagg caccaccaca tcattattgg cgtaaatatt ccacacaaac tggaaatcat
     7081 cgctgttata gttgttggtc tgtcgcaaaa taagcacggc aattaatgag ccagctttaa
     7141 ttgccacccc gcccgcactg ctcacaggcg tcaaataaag cgccaccggc cacggcttat
     7201 ccgttctcga attataaaca acgcgcggcg tttcgctggt ggtcggaaat ggatagctac
     7261 tgccactata ttttacggtc ccggaaaaat tagataacac gccgccataa gccgagcctc
     7321 gttgcagtgt gacatagtct gtaatggttt ccggataatc gttatggcaa aagatttgcg
     7381 tcgaaagatc tacgaccagg ttttgcccca cattcacggc aggcgcaagg tttacataaa
     7441 cattagcgct gccaccgcca atagggatag ctgtaccatt ggcggtttta caggcgaatg
     7501 accaggcatt taccgaccag cccatcagca gtacagcaaa cagggtaata actcgtttca
     7561 ttacaatcat ctcttcgggt tcagctgtag gtataggtga tgctaatcac tgcctgaatg
     7621 gttccctgag tggctccgcc atttactgtc aatgctctga cctgtaacgg gaagtgcgct
     7681 gattgtgagg aatcatccac ctgaactgtt ttggttgcgc cagtattcaa tgtgttgcca
     7741 ctgtcatcct gtagctctaa ctggatgttt tgcgcggtcc cctggttttt ataatatccg
     7801 gtactgtcgg ctgccccgct gaagctggca gtgaccctcg acgttcccac cggacaatta
     7861 gtcaactcaa gcgcaacatc atgccaggcc gatgccgccc cggcagacat cagactgaaa
     7921 gaataaagat cgccgagatc aaccgtggca ttggtggtgg aaactgtgca cggtttggcg
     7981 acgaccttac cgttcaccgt gatggtgaca tcggctgcct gtatcgtcgc acttgcgagc
     8041 gccaatattg ccgccaatac atacccacgt ttgcaccatt tcatgagcat ctccagttac
     8101 tgatattcaa gagtgaaggt agccgtggca ttgatatgcc ctgcagtgac aggcacctgt
     8161 gtcgccatta gccgggcgta aaaattcagc gtatttggtt tacccggcgt cagggtcgtc
     8221 cacgaaagcg cggacgatgg agcattaagg ggtatttgat tttgctgctc attcagaagc
     8281 tgtattccca gtcccgaagc cgctgacacc gtattttcaa gtgcaagcag attggcattg
     8341 tggctatctg caacgccggt aaacccaact tttacggcag aaacggcatt accacagggt
     8401 gacagcaaaa tacgaaatgg aacaacagga gtcgtcgcgc caatgttgtt aaattgcttc
     8461 gccgcgtttt ccatcagatc aacagtaaaa ttggttgatt cagcggccac actacagccg
     8521 ttatccctga catagccgcg gatagtaatc gtgctatccg cagccaaagc gtgactcacc
     8581 gccagccaca aaaaagcgca cagaagataa aaaggtttgt ttctcatcac gcccccttaa
     8641 cgacattcag ctgatagctg ggttaataac tgctgctgac tctctggtgg cagttgataa
     8701 ttggcgacac agtgagcatt ttcctcttct ccccatttca cctgaacttt tcccgctaaa
     8761 ggcattccgc tgaggtaaac ctgaccatta tccgcaacaa tgccgctact ctggctactc
     8821 tctgatgtca ccatcgcccc aaacggcagc ggcttattat tgtgggtcag cgtcatgagc
     8881 agttttatcc caacgcgcgc tttaaactct gctcgcacga tcgccccacg agtgggaaca
     8941 acgttagcaa ccgcgttatc taaatcgacg ttatcagcca gggtattggt atccagcgcc
     9001 actctatttt cccgatattc agtggcataa ggcagcacgg cataaccacg ccagtcggta
     9061 cgcacccccg tctggttttc gacttttgca tcttttgcgc caggcgcttt aacaagcacc
     9121 accgtatcgt ttaacggctg ccccagcgtt acgccattgg catgagccag taccccaccg
     9181 ctgactccgt aatagagctg cttaatatca tcactatggc tgtaaccgat attggcattg
     9241 ccgtaaccac cgcgataatt cagcgtggcg tagcctgtgc ttccgctatt accatcgcct
     9301 cccccggcat agccggtttg cacgctatag ctgaggttgt tgtcttccag caacgtaccg
     9361 tatacaccag ccagattggt cattcgaccg ttgagatcgt gtgacatgct gtagctggca
     9421 ctggcatgtc gccactgaga tttactgtca gaacgcagcc agtggctgaa aggaatattg
     9481 acgttaagcg ctaacatctg atcacgtcct ttttgccagg cgtttttcgt caggctatag
     9541 ctgagcgtcc agttgatatc ttcgaacgca gtatttaatc cagcctggaa ttgctcatcg
     9601 acattactcg ttccccaata agtttgatgg ctaccactca aatacagtgt tgatgagcgc
     9661 ccgagttgct gagtaacggt gagttgtaat ttcccgcgtt tgttataagc gaggttgtaa
     9721 tagtcggtga atttcggctt aacctgaata actccgtcct gtgtttcgat gttgtagcca
     9781 ttcattcgac tgtatgttgt atcagcgaaa ttaaaatatc cgctggtcga ataacggtaa
     9841 cccactaact gaatattcgt gcctgactca ttgagcgatt tgttatagag aaaacgcacc
     9901 gattgtccgt catgctgact gtcatcggga agtgtggaat tagcctgcgt catatccaca
     9961 gacagagcgc ccagtgcccc catgtttttc ccgataccga aattaaaagc acgataacga
    10021 tccgccagtt gcgttccacc atatattgtc cagccagccg gaaggccgtg gagtaatgta
    10081 ctctggaaaa agcggggttt ttcctgctgc gcatttccac tacggtattc tcctgccgta
    10141 atggaataac gagtatgccc ttcacgttgc aaaagcggga ctgacgaata gggtacggta
    10201 aaaatctgcg tgctgccgtc agcctctttg atcgttacct gcaagtcacc actattacct
    10261 gcggcataga tatcgttgat ggtaaaaggc cccggtggca ccgtactatt ataaatgtca
    10321 tacccatttt gtttaatagt gacctgtgca gtaccacgag caataccgtg gatcaccggg
    10381 gcaaatcctc tttggctatc gggtaacata ttgtcatctg aggccaattg tgcgccgcga
    10441 aagttaatac catcgaaaat atcgccctga gtataaccat cacccagcgt cagccgggaa
    10501 cgtaacggta ttatgtctcg ctcaagccag gtattgatat gctgccattt atttttgcta
    10561 cctgatgatc tgtcgctact gttataactc caggtggtat tgtcgcgtaa acgccacgca
    10621 ccaatattta acccactctg taggtttaaa tatgcataat ggctgttacc cccaatccga
    10681 ttctgtacac tatttccgct gaaattataa ttgagcaatc cggcattaat accgggatcc
    10741 cataactcag gaggaatata accacgcgcg cgattactca taaatgcctg agggatcgtc
    10801 aggttgagtc gctgctgacc aacatctaaa tgcgcagtag cgtcatggat tattgaggtt
    10861 aatggcacgc aggcatcatc cgccagcaga ttcataccgg agacagaagc cgtattcagc
    10921 cccatactgg caagttgcgc gcgtgtcagg cagggaacaa tcccttgttc gctgtcgccc
    10981 gtattaaatg tgacatcacg cgttgccata taaccattat tcaaatagat atcgacacga
    11041 tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg ataaatcagc cacagcctgg
    11101 ggatcatccg ctaaaaagcg cgggttaaaa tagagttcgg cagatgacaa aggtgcctgt
    11161 gcggcaaaag cacaggcaac aacgagccgg acaaaaaaac cagccaaacg atgcttacga
    11221 atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat tcagatatga cattattcct
    11281 ccggtttaaa atcacccggc agtcaattct ttttattcag gcgaaaaatt ccccctgcgt
    11341 tattccatta cgcccgtcat tttgggggta agtgcgccat aatcatttat tgttcggtaa
    11401 gtaatattgc ttcccgcatc agaaggcaat ttaaccgtgc tttcgcccat tggaggcacc
    11461 aaggcatttt caagaacccg ggctccggca ttcaactctg ttaccgtcag gtaatagggt
    11521 gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa atcttaattt ttctgcggcc
    11581 tgatcgggtg gcaacgctaa tttagccggg cgatagtaca gtttaatgcg gctgataatt
    11641 gcgagctgta gcgtattctc agtcaatttt gatttatcca ttgacggaat cgctttaaca
    11701 ttcatccaga ataaactttc ccgatcctgt ggcaattggt tatttgttgc atcaagaata
    11761 cgtaaggtat tctctttttt tcccttcatc gcaaacagag gaggcgtcac gataaaacga
    11821 ccatccttta caccatcggc attttccacc catgattgaa ttaaataggt actattttca
    11881 tcattatttg tcacggcaag ttgcacttgt ttttgccctg ccggataaat tacgcgagtc
    11941 gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca tcattgccat gaacatcagg
    12001 atacctgcca gcaagcagaa tgttatttcc tgcgatttcc ttacattgac gtttttatta
    12061 ctcactgtcc tgttcctgtt atcacattat ctgctgaaca attactgata ggttaaagag
    12121 aaccaggcct gggcattggc gatgccgcca gtaacccgac gcccggtagc acgatatttg
    12181 gcgatgaaat gtagcgaagt agagcctgag taaagccgtt tccagtttgc tggaggacga
    12241 ttaatcggta cgaggtttcc ttcatcatca aacaacgcta cgccaatatt ggtggctatc
    12301 cctggcccct ctcccacgga aagcacatcc ggatttttac catccgcgac accgtgaaac
    12361 gccacaccta cacgttcact caccaccgtg ctacattccc gtaaatgaat aacaaaaggc
    12421 accggtgcgc tatcttcccc taccgcatga aaccggttac tgctgatttg ccccatattg
    12481 accgtcattt gtttatcacc ggcttcaatc cggcaagttt ccgcaataat gacgccctga
    12541 aatttcatat ttccgccggg cagcgtggta ttccatttat ttccggccag agcaaacatt
    12601 ggcagcaacg acgctaatag aaataatctc ttccttttca tcgttatttt tatcgcacaa
    12661 gggtgggcat ccctgcccgt aatgacgtcc ctgaacctgg gtaggttatt gatactgaac
    12721 cttgaaggtc gcatccgcat tagctgcgcc aggggttgcg gcaccggttg caaaataacg
    12781 cgcctggaac ggaatggtgt tggttccgtt attcagggtt gtttgtgcac taaatgtcgc
    12841 accgtccagc gccagcgcag cgcccgttct gtccaggatc tgcacaccaa cttttgttgc
    12901 gctacccgca gctgaactct gcaaagctaa tacgttagga tgagtgccat caattgcggt
    12961 accaaggaag gcaatagctg ctttgtctgc aacagtagtg tcacaatcgt tcagttgaat
    13021 gttaaaaccg acagcagaac tggttgctcc ttcttgtgcc agcgatgcgg tacgaacctg
    13081 tcctaactga acggtttgat caacagagcc tgcatcaact gcgcaagcgg cattaacaac
    13141 ttccccttta aagtgaacgg tcccaccatt taccgtcgta gtatcggcca gagccgctgc
    13201 ggaactgagg gacagagccg acagaacaac gattgccaga gttttaattt tcatgctgct
    13261 ttcctttaaa aaaactattt ctaaatcgac atgggcagtc gttctgtaca ctttgttttg
    13321 tcaacgagtt tgactgccaa cactgcacag ttttccccca a
//
LOCUS       DAPHDW010000124        13254 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.126, whole genome shotgun sequence.
ACCESSION   DAPHDW010000124 DAPHDW010000000
VERSION     DAPHDW010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13254)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13254)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13254
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.126"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..629
                     /locus_tag="RH057_003496"
     CDS             <1..629
                     /locus_tag="RH057_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HDV3129813.1"
                     /translation="SAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPY
                     VNLFKADLSFSKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVIC
                     TDSNMANVNFNNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEEN
                     IPIDSDTLVTRQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(783..2432)
                     /locus_tag="RH057_003497"
     CDS             complement(783..2432)
                     /locus_tag="RH057_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HDV3129814.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(2583..3932)
                     /gene="ghxP"
                     /locus_tag="RH057_003498"
                     /gene_synonym="yjcD"
     CDS             complement(2583..3932)
                     /gene="ghxP"
                     /locus_tag="RH057_003498"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HDV3129815.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLVGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(4478..4942)
                     /gene="soxR"
                     /locus_tag="RH057_003499"
     CDS             complement(4478..4942)
                     /gene="soxR"
                     /locus_tag="RH057_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HDV3129816.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            5028..5351
                     /gene="soxS"
                     /locus_tag="RH057_003500"
     CDS             5028..5351
                     /gene="soxS"
                     /locus_tag="RH057_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HDV3129817.1"
                     /translation="MSHQKIIQDLIAWIDEHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(5354..6940)
                     /gene="pdeC"
                     /locus_tag="RH057_003501"
     CDS             complement(5354..6940)
                     /gene="pdeC"
                     /locus_tag="RH057_003501"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
                     /protein_id="HDV3129818.1"
                     /translation="MSHRARHQLLALPGIIFLVLFPIILSLWIAFLWAKSEVNNQLRT
                     FAQLALDKSELVIRQADLVSDAAERYQGQVCTPAHQKRMLNIIRGYLYINELIYARDN
                     HFLCSSLIAPVNGYTIAPADYKREPNVSIYYYRDTPFFSGYKMTYMQRGNYVAVINPL
                     FWSEVMSDDPTLQWGVYDTVTKTFFSLSKEASAATFSPLIHLKDLTVQRNGYLYATVY
                     STKRPIAAIVATSYQRLITHFYNHLIFALPAGILGSLVLLLLWLRIRQNYLSPKRKLQ
                     RALEKHQLCLYYQPIIDIKTEKCIGAEALLRWLGEQGQIMNPAEFIPLAEKEGMIEQV
                     TDYVIDNVFRDLGDYLATHADRYVSINLSASDFHTSRLIARINQKTEQYAVRPQQIKF
                     EVTEHAFLDVDKMTPIILAFRQAGYEVAIDDFGIGYSNLHNLKSLNVDILKIDKSFVE
                     TLTTHKTSHLIAEHIIELAHSLGLKTIAEGVETEEQVNWLRKRGVRYCQGWFFAKAMP
                     PQVFMQWMEQLPARELTRGQ"
     gene            7370..7651
                     /locus_tag="RH057_003502"
     CDS             7370..7651
                     /locus_tag="RH057_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HDV3129819.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
     gene            complement(7750..8286)
                     /gene="ssb1"
                     /locus_tag="RH057_003503"
     CDS             complement(7750..8286)
                     /gene="ssb1"
                     /locus_tag="RH057_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HDV3129820.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            8540..11362
                     /gene="uvrA"
                     /locus_tag="RH057_003504"
     CDS             8540..11362
                     /gene="uvrA"
                     /locus_tag="RH057_003504"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HDV3129821.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(11397..11753)
                     /locus_tag="RH057_003505"
     CDS             complement(11397..11753)
                     /locus_tag="RH057_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HDV3129822.1"
                     /translation="MTISELLQYCMAKPGAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(11757..12173)
                     /locus_tag="RH057_003506"
     CDS             complement(11757..12173)
                     /locus_tag="RH057_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HDV3129823.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMERFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(12284..12997)
                     /gene="aphA"
                     /locus_tag="RH057_003507"
     CDS             complement(12284..12997)
                     /gene="aphA"
                     /locus_tag="RH057_003507"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HDV3129824.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPVT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
BASE COUNT         3441 a         3455 c         3233 g         3125 t
ORIGIN      
        1 gctccgctat gcatgttcaa ttctacaatg cagcaatgaa tagagccaat attaaaaata
       61 cctttcttga ctattcaaat ttttatatgg cgtacatggc tgaggtaaat ctttataaag
      121 taatagcgcc atatgttaat ttatttaaag ccgaccttag tttctctaaa ctcgatttaa
      181 ttaactttga acatgctgat ctgtctcgcg tcaatctgaa caaagcaatc ctccagaata
      241 taaacttaat tgatagcaaa ctcttttgta cgtggctaac aaatacattc ctcgaaatgg
      301 ttatatgtac cgactctaat atggctaatg ttaattttaa taatgccaat ttaagcaact
      361 gccatttcaa ctgttctatt ttaacaaaag cctgtatgtt taatacccgt ctctatcggg
      421 ttaattttga tgaggctagc gtccagggaa tgggcatttc catactccgt ggggaggaaa
      481 atatccccat tgatagtgat accctggtaa cacgacagaa attctttgaa gaagattgta
      541 cctctcatac tggcatgtca caaactgagg ataatattaa tgcagtcgct atgaagatta
      601 ctgcagatat tatgcaacac gcagattgag gttaaatcga agaacagcca atgaattgac
      661 tttacaagag agataactta tttgttttag aatcatgaat gttagcccga gctaacctgt
      721 taaaggtgca aaagattgca cctttttcag attacctcgg gcctggttac gtcgctttcg
      781 gcttactgat tttcctcaat tagcaacgct tcaagcaaat cgagatcgtg cagtaatttt
      841 tgcagcgttt cgttgctgat ctcccgcgtg gcgcgcaggt ggtacagttc agcacgttca
      901 gaacgcaatg ccgccagacg gaagcgacgc tcaaggttct cttcctgcac ggaactttca
      961 acgtcgttac gtccatcggc gcgacgacgc aggttaccaa tgacgcgaga actgacctcc
     1021 gtaagcagct ggttatcgat attctcttcg gtatccgccg ccagacgctc ctccattttc
     1081 tggatcgcca caatcgccac ttctgccgtt gccgctcgcg caatacgttc ctctttcaat
     1141 tgttgcgaat gatccgcgac ttcaatgtgt tgtagcagaa taggcaacat caccacgccg
     1201 acaaacagcg agaagagaat gacgccagcc gccaggaaca ccagctcata gcgcgccggg
     1261 aagacgttac catccggcaa gagcagcggg atagagagca caccggccag agtgatcgcc
     1321 ccacgcaccc cagcgaaaga cgcgatcagg atttctcgtg tggtccacga accaaactcc
     1381 atcggcttct tcttcaggaa gcggttgctg aactttttca tcgtccacag ccagccgaaa
     1441 cggaccagca tcagcgccgc atatatcaga ataatatcgg taaacagcat ccagatttcg
     1501 acgttagggt cgatttctgc cgccatcagc gacgtctcca gaatacccgg tagctgcaga
     1561 cctaacagca ggaacaccat gccgttaaag acaaattcca gcatcgccca ggtgctgttt
     1621 gcacgcaggc gcattgccag cggcgcacgg cgcatcacac cggagcgggt gatggtcatc
     1681 ccggcggcaa ccgcagcgag gatgccggaa acgccaatat gttcggcaat cagataagaa
     1741 gcgaatggca gcaggaacag caggacgatc tgcgtcgcgg gttcatcacc gccccagcgg
     1801 ctgaggaatc gcagcgagcg accgtacagc cagctaacca caaaaccggc gagaataccg
     1861 ccaatggcta ctttcataaa ttcgaccgtc gcaccgccga cggtgaagat catcgtcccc
     1921 atcgccactg ctacggcaaa cttcaacgac accaggccgg aagcgtcatt catcaacgct
     1981 tcgccctgca aaatgcccat gatttttttc gggatacgcc cttcgccaac aatcccggag
     2041 agcgccacag catcggtcgg agaaagtacc gccgccagcg caaaggcggg gatcagcgga
     2101 atgcccggca ccacccagta aataaggaag ccaatgccga ccacggtgac caccaccagc
     2161 gccagcgcga ggccaaaaat ctctcgacca tgttcgagaa attcacgggt cggcgttttc
     2221 cagccatcag cgaacagcaa cggcgggata aacaagacta aaaagagttc aggatcaaac
     2281 tccacatgca aaccaaacgt cggccacgcc agtagcgcac cgatggcgat ttgcataagc
     2341 ggaagcggga tctgaaaggg catgacacga gtgaccaccc cggacagcga gaccacgagg
     2401 gtcattatca gtatggtgaa gaagatttcc atgcgttccc tgtttgcgat gttttttatg
     2461 atctgagaaa gacgttatgc ctgaattctc attttttcag aatatcgcaa aagctcacac
     2521 aatgtgcgga gaaaataaaa acgggcagcc attgccaccc gttttcagaa tccgatgaaa
     2581 gattagatag cccagccacc cgcatagaag gtcaccagcg ccacggcgat aacgaccgta
     2641 ccgatgttca acttgcgcca ttcgccggaa accagacgac caatcaccag agtcgcgaag
     2701 ccgatcatga tgcctgttac gatgttacag gtcagcacga tgaataccgc cgtaaccaga
     2761 cccgccatcg catcaacaaa atcagcaaag tcgattttcg ccacgttgct cagcatcagc
     2821 aggccaacgt acatcagcgc cggagccgtt gcataccccg gaacgaggta agagagcgga
     2881 gagaggaaca gaatcagcag gaacagcacg ccaacggtga tagccgtcaa accggtttta
     2941 ccgcccgccg ccgtacccgc cgcagactcg atgtataccg ctgccggagc tgcacccacc
     3001 aggccagaga aaacgctgct catggagtca gtggtcagtg ctttcccacc gtcgatgatc
     3061 tgcccatctt tatccagcag gttcgcctgg ccggcgacgg cacggatagt tccggtggca
     3121 tcaaatactg ccgtcatcac cagcgccaga acgcttggca ggactacagg attcagcgcg
     3181 cccataatgt ccaggctgcc aatcagtgaa ttgccgtttt catcgctcaa tgaaggcatg
     3241 gcgaaaacgc cggagaaatg gacgttagga tcgaagatca aaccgacaat tgagataccg
     3301 ataatggtca gcagaatgcc accagggact ttcagttttt ccaggccgat gatcaccgcc
     3361 agacctacca gtgacataat caccgggaag gtcgcgaaat cacccagcgc aacgggcaga
     3421 ccatcaagcg ggtttttaat caccagaccg acaccattag cggcaatgag cagcaggaac
     3481 agaccgatac cgatccccgt gccgtgcgcc acaccgtgag gcaagttgcg caaaatccag
     3541 ctacggatac ccgtggcaga aattaccgta aacagcacac ccatcaggaa cacggcaccc
     3601 agcgcgacag gtacgctaat atgttgcccc agcaccaggc tgaatgcggt aaatgccgtc
     3661 agggagatgg cgcaaccaat cgccaacggc agattagccc acagacccat cacgatagaa
     3721 ccgagtccgg caaccagaca ggttgcaacg aaaactgccg caggcgggaa gcctgcttta
     3781 cccaacatac ctggaacgac gatgaccgag tagaccatcg ccagaaacgt tgttaacccg
     3841 gcaaccactt cctgacggac agtgcttcca cgttgtgaaa ttttaaacca ggcgtcgagt
     3901 gaaccgccgg tacgcgctga tggcgtagac ataagaaaac atcccctgag agtttaattt
     3961 tcgtcaagat gcgtggcgga caatcccctg ccagcgaaac gttatcttcc tgctccaggt
     4021 ttgcgacaat ttgctgcgtc gcaaaaaagc aaacgtttag ctccgcgcat acaaaacggg
     4081 tggtaaaaaa agcaaacgat tatccggcga tcatagcggc tttttcaacc gaagtttgct
     4141 gcttttttct cttttattgt cttgacgatg aaaaaccagg caattgatgc gcaaacgtta
     4201 tcgtcggtgc gcaagattgc agcgttatcg agccgtatac cgttcaggat tgcgataaaa
     4261 aatcgtctga gatgacaaaa atattgcggt taaaaagtat acttttacac tggcgaagaa
     4321 acgtcggtga catacaatta aagcatcaac accaaccgga acctccacca cgtgctcgaa
     4381 tgaggtgtgt tgacgtcggg ggaaaccctc ctgtgtacca gcgggataga gagaaagaca
     4441 aagaccggaa aacaaactaa agcgcccttg tggcgcttta gttttgttca tcttccagca
     4501 agcgtgcgcc ggtaccttct tctcctaagc ggtcgcccgg gttacgcaac gggcaatcac
     4561 tgcgcgaaag gcagccacaa ccaatacatc cgtccagttc gtcacgcagc gctactaagg
     4621 tatgaatgcg ccgatccaac tcttctcgcc attgggacga aagctgtttc cactctttcg
     4681 cacttaacgt atgcccttcg ggcaacacgc caaacgcttc accaatggtc gccagcggaa
     4741 tgccaatacg ctgagcaatt ttgataattg caacatatcg caacacatca cgtttatatc
     4801 gccgctgatt gccgctgtta cggatactgg taatcaaccc tttactttca tagaaatgca
     4861 gcgccgatac cgccacaccg ctgcgtttcg ccacttcgcc gggggttagc agcgctttaa
     4921 tgcggggtaa tttcttttcc ataaatcgct ttacctcaag ttaacttgag gaattatact
     4981 ccccaacaga tgaattaacg aactgaacac tgaaaagagg cagatttatg tcccatcaga
     5041 aaattattca ggatcttatc gcatggattg acgagcatat tgaccagccg cttaacattg
     5101 atgtagtcgc aaaaaaatca ggctattcaa agtggtactt gcaacgaatg ttccgcacgg
     5161 tgacgcatca gacgcttggc gattacattc gccaacgccg cctgttactg gccgccgttg
     5221 agttgcgcac caccgagcgt ccgatttttg atatcgcaat ggacctgggt tatgtctcgc
     5281 agcagacctt ctcccgcgtt ttccgtcggc agtttgatcg cactcccagc gattatcgcc
     5341 accgcctgta attttattgc ccgcgcgtta actcccgcgc gggtaattgc tccatccatt
     5401 gcataaacac ctgcggcggc atcgccttcg caaagaacca tccctggcaa tagcgcacgc
     5461 cgcgtttgcg cagccagtta acctgctcct cagtttcgac gccttcagcg atcgttttta
     5521 accccaggct gtgcgccagc tcgatgatgt gttccgcaat caaatgactg gttttgtggg
     5581 tggtcagcgt ttcaacaaac gatttgtcga ttttcaaaat atcgacattc aatgatttaa
     5641 ggttatgcaa gttagagtag ccaataccaa aatcatcaat tgccacttcg taacctgcct
     5701 ggcggaaagc cagaataatc ggcgtcattt tgtcaacatc aagaaatgca tgttcagtca
     5761 cttcaaattt aatttgctgc ggacgcaccg cgtattgctc tgttttctga ttgattcgcg
     5821 ctatcaaccg tgacgtatga aaatcggagg ccgacaggtt aatagaaaca tagcgatctg
     5881 catgtgttgc caggtaatcg cccagatcgc ggaagacatt atcaataaca taatcagtta
     5941 cctgttctat catcccctcc ttttctgcca gcggaataaa ctctgccgga ttcattattt
     6001 gcccctgctc accaagccaa cgtaacaacg cttcagcgcc gatacatttt tctgttttga
     6061 tatcgattat tggctggtaa taaagacaaa gttgatgttt ttcgagggcg cgttgcaatt
     6121 tacgtttggg agataaatag ttttgtcgaa tacgtagcca gagtaatagc agaacaagac
     6181 tccccaaaat accggcgggc aacgcaaaaa taagatgatt ataaaaatgg gttataagac
     6241 gttgatatga agtcgcaaca atggctgcaa ttgggcgttt tgtcgaataa actgtcgcat
     6301 ataaatagcc atttctttgt acggttaaat ccttcaaatg aatcagcgga gaaaacgttg
     6361 ctgccgaggc ctctttgctt aacgagaaaa aggttttcgt caccgtatca tacacacccc
     6421 attgcaatgt cgggtcatca gacatcactt cactccagaa gagagggttg ataaccgcca
     6481 cataatttcc ccgctgcata taggtcattt tatagccaga gaaaaaaggc gtatcgcggt
     6541 aataatagat agaaacgtta ggttcacgct tataatcggc cggtgcaatc gtatagccgt
     6601 ttacaggcgc tatcagcgat gagcataaaa aatggttatc acgggcatag atcaattcat
     6661 taatataaag atagccacga ataatattca acattcgctt ttgatgggct ggagtgcaaa
     6721 cttgcccctg atagcgttca gctgcatcgc tcactaaatc tgcctggcga atgaccagct
     6781 cggatttatc cagtgccagt tgagcaaagg ttcggagctg attattcact tctgattttg
     6841 cccaaaggaa ggcaatccat agcgaaagaa tgatgggaaa gagaactaaa aagataatgc
     6901 ccggcaacgc cagtaattgg tgtcgtgcac gatgactcat tgctatcaat ttcctgcctg
     6961 tttatacccg gccattgcaa ggtgaaaacc ctgggtaccg acgtctttag ataaatctta
     7021 cggtataaat agcacttttg cctgatttga aattgatacc tttctaatcg tcacctggat
     7081 gaaaataatt cattgatatt caaaattaat ttcctacagg aatgcagagg cggcgggaag
     7141 atatgccctt aatgccactg aaaaaccaca taatctgtac agcaataaaa gtcacggcct
     7201 aatatggcta acaaacaatc aattgtgata tagttcacaa aattaatgaa acaaacagag
     7261 tgtttcattt ttgtgttctg caagccccgc gatagccaga gtgtaccacg cctcccgtga
     7321 acaacgccgc gctgtccagg gcatcggctc ttttcagagg aattgtttga tggctaccct
     7381 caccactggc gtggttcttc ttcgctggca acttcttagt gccgtaatga tgtttctggc
     7441 cagcacactc aacatccgtt ttcgtcggtc tgattatgtc gggcttgcgg tgatcagcag
     7501 cggtctgggc gttgtttccg cctgttggtt cgcaatgggg ttgcttggca tcacaatggc
     7561 ggacatcacc gctatctggc ataacatcga gtcggtgatg atagaagaga tgaatcagac
     7621 accgccacaa tggccaatga ttttgacctg atacgtagaa gcctccataa cggaggcttc
     7681 ttttttatgg ctggcagatg ctttaatcat ccaccttaaa acaatataac ctattgtttt
     7741 aatgacaaat cagaacggaa tgtcatcatc aaagtccatc ggcggctcgt tagacggcgc
     7801 tgccggagcg gactgctgcg ggcgagactg cgcgccgccg ctgaactgat tgccaccctg
     7861 cggctgctga ggctgacccc aaccgccctg cggctgacca ccaccgatat taccacctgc
     7921 cggagcgcca ccaccctgac gaccacccag catctgcatg gtgccgccaa cgttcaccac
     7981 gacttctgtg gtgtagcgat cctgaccgga ttgatcggtc catttacggg tacgcagctg
     8041 accttcgata taaacctgag aacctttacg cagatattcg ctggccactt ctgccagttt
     8101 gccgaacagc acaacgcggt gccattcagt ctgttctttc atctcgccgg tcgctttatc
     8161 acgccaggat tcggaagtag ccagcgtaat gttggcaact gcgccaccat ttggcatgta
     8221 gcgtacttcc gggtcctgac ccagattacc aacgagaata accttgttta cgcctctgct
     8281 ggccatgttc gtgtctcctg aaaaaaatcg ttctgaataa gtgtaaacgc gcgattgtac
     8341 cattaccaat agcgctttta ctatgttgtg acctcggttc cgggaaacaa acctggccag
     8401 acattgttac acaacactcc gggtaatgca ttccaatact gtatattcat tcaggtcaat
     8461 ttgtgtcata attaaccgtt tgtgatcgcc ggtagcacca tgccaccggg caaaaaagcg
     8521 tttaatccgg gaaaggtgaa tggataagat cgaagttcgg ggcgcccgca cccataatct
     8581 caaaaacatc aacctcgtta tcccccgcga caagctcatt gtcgtgaccg ggctttcggg
     8641 ttctggcaaa tcctcgctcg ctttcgacac cttatatgcc gaagggcagc gccgttacgt
     8701 tgaatccctt tccgcctacg cgcggcagtt tctgtcactg atggaaaagc cggacgtcga
     8761 tcatattgag gggctttctc ctgccatctc aattgagcag aaatcgacgt ctcataaccc
     8821 gcgttctacg gtggggacaa tcaccgaaat tcacgactat ttgcgtttgt tgttcgcccg
     8881 cgtcggcgaa ccgcgctgtc cggaccacga cgtcccgctg gcggcgcaaa ccgtcagcca
     8941 gatggtggat aacgtgctgt cgcagccgga aggcaagcgt ctgatgctgc tcgcgccaat
     9001 cattaaagag cgcaaaggcg aacacaccaa aacgctggag aacctggcaa gccagggtta
     9061 catccgtgct cgtattgatg gcgaagtctg cgatctttcc gatccgccga aactggaact
     9121 gcaaaagaaa cataccattg aagtggtggt tgatcgcttc aaggtgcgtg acgatcttac
     9181 ccaacgtctt gcggagtcgt ttgaaaccgc gctggagctt tccggtggta cagcggtagt
     9241 ggcggatatg gacgacccga aagcggaaga gctgctgttc tccgccaact tcgcctgccc
     9301 aatttgcggc tacagtatgc gtgaactgga gccgcgactg ttttcgttta acaacccggc
     9361 aggtgcctgc ccgacctgtg acggccttgg cgtacagcaa tatttcgatc ctgaccgcgt
     9421 gatccaaaac cccgagctgt cactggctgg cggtgcgatc cgtggctggg atcgccgcaa
     9481 cttctattac ttccagatgc tgaaatcgct ggcagatcac tataagttcg acgtcgaagc
     9541 gccgtggggc agcctgagcg cgaacgtgca taaagtggtg ttgtacggtt ctggcaaaga
     9601 aaacattgaa ttcaaataca tgaacgatcg tggcgatacc tccatccgtc gtcatccgtt
     9661 cgaaggcgtg ctgcacaata tggagcgccg ttataaagag acagaatcca gtgcggtacg
     9721 tgaagaatta gccaagttta tcagcaatcg cccatgcgcc agctgcgaag ggacgcgtct
     9781 gcgtcgggaa gcgcgccacg tttatgtcga gaatacgccg ctgcctgcta tctccgacat
     9841 gagcatcggt catgcgatgg aattcttcaa caatctcaaa ctcgctggtc aacgggcgaa
     9901 gattgcggaa aaaatcctta aagagatcgg cgatcgtttg aaattcctcg ttaacgtcgg
     9961 cctgaattac ctgacgcttt cccgctcggc agaaacgctt tccggcggtg aagcccagcg
    10021 tatccgtctg gcgagccaga ttggtgcggg cctggttggc gttatgtacg tgctggacga
    10081 gccatctatc ggcctgcacc agcgcgataa cgagcgcctg ttgggtacgc ttatccatct
    10141 gcgcgatctc ggtaataccg tgattgtggt ggagcacgac gaagacgcaa ttcgcgccgc
    10201 tgaccatgtg atcgacattg gcccgggcgc aggtgttcac ggcggtgaag tggtcgcaga
    10261 aggtccgctg gaagcgatta tggcggtgcc ggagtcgttg accgggcagt acatgagcgg
    10321 caaacgcaag attgaagtgc cgaagaaacg cgttccggcg aatccggaaa aagtgctgaa
    10381 gctgacaggc gcacgcggca acaacctgaa ggacgtgacg ctgacgctgc cggtgggtct
    10441 gtttacctgc atcaccgggg tttcaggttc cggtaaatcg acgctgatta acgacacact
    10501 gttcccgatt gcccaacgcc agttgaatgg ggcgaccatc gccgaaccag caccgtatcg
    10561 cgatattcag gggctggagc atttcgataa agtgatcgat atcgaccaaa gcccaattgg
    10621 tcgtactcca cgttctaacc cggcgaccta taccggcgtg tttacgcctg tgcgcgaact
    10681 gtttgcgggc gtaccggaat cccgtgcgcg tggttatacg ccaggacgtt tcagctttaa
    10741 cgtccgtggc gggcgctgcg aagcctgtca gggcgacggt gtgatcaaag tagagatgca
    10801 cttcctgccg gatatctacg tgccgtgcga tcagtgtaaa ggtaaacgct ataaccgtga
    10861 aacgctggaa attaagtaca aaggcaaaac catccacgaa gtgctggata tgaccatcga
    10921 agaggcgcgt gagttctttg atgccgtacc tgcactggcg cgtaagctgc aaacgttgat
    10981 ggacgttggc ctgacgtaca ttcgcctggg gcagtccgca accacacttt ctggtggtga
    11041 agcccagcgc gtgaagctgg cgcgtgagct gtcaaaacgc ggcaccgggc agacgctgta
    11101 tattctcgac gagccgacca ccggtctgca cttcgccgat attcagcaac tgctcgacgt
    11161 actgcataaa ctgcgcgatc agggcaacac cattgtggtg attgagcaca atctcgacgt
    11221 gatcaaaacc gctgactgga ttgtcgacct gggaccagaa ggcggcagtg gcggcggcga
    11281 aatcctcgtc tccggtacgc cagaaaccgt cgcggagtgc gaagcttcgc atacggcacg
    11341 cttcctcaag ccgatgctgt aatcgttaag gccgctttct gagcggcctt ttcctttcag
    11401 agttgtacca gcaatttacg cttttcttcc ggtagtaaat tcactgcctg ctgataagac
    11461 gcatccacca gataatagat ttgcgaatcc ggcagcgaac cgtcgagata cacggtgctc
    11521 cagtgcgctt tattcagatg gcggctagga cgcacatcgc tgtgctgctg acgtagcagc
    11581 tccgccagct ccgggctggt tttcagcgaa acagctgggc gattttctac ttctttcacc
    11641 atcgcaaaca gtacatcttc cactttgatc tgcgtcgctt tccagtcgtt atgcacgctc
    11701 tgttctgcgc ctggttttgc catgcaatat tgtagcaact ccgaaatggt cattttttac
    11761 tccccttgta gtgtcgcgat gatgcgacgc gatccgccgt ggatgcgatg ttcccccagc
    11821 cagatgcctt gccaggtgcc ggtctgaata cgccctttat gtaccggcaa tacaagcgat
    11881 gttcccagca ttgaggattt gatatgagaa ggcatatcgt ctgctccctc atagtcatgc
    11941 tcataatttc cgttgtcggg aacggtgcgg aggaaaaaac gctccatgtc gtggcgtacg
    12001 gtgggatcgc agttctcatt aagtgtcaga gaggcggagg tatgttgcag caacagatgc
    12061 agtaagccga tgttaacgcg cggcatatca gccagctgat tcagaatttc atccgttacc
    12121 agatgaaacc cacgagattt ggcgctaagc gtgagcgtct tttgatacca catgtgctgc
    12181 tccttgataa aactctctta atcagtttgc agcaagacag cgaaaggata aaggtgtgat
    12241 taaaaaaaca gcattcggga gagcgtcacg ctctcccgct ctgtcagtat tctgaattga
    12301 cgatcacctc ttcaccaaac gcacccgctt gtggcaaggg tttgtaggta gagttggagg
    12361 cgcgcagaat gcggatacca cgagcgccga catcgcgtgc ggcggtaata tcgttatcgg
    12421 aatcgccata aaaaattcgg atatttttat cctgcagcca ttgcgatttt gtattttgcc
    12481 ctggtttatc gcccgcaaag atcaccggat tcatgttggt gacaggaata tgaaaattat
    12541 ccgccagcgt ttttgaaacc gtttctgttt tcgtcgggct acgaccagtc acaaagaaga
    12601 tcgcgtcacc gcggcgtaca tgcatatcaa tcagctggcg agcgacctct tttggaatgc
    12661 tgaattcatc ccagccattg ttcatttttt cccagaacac aggatttttc agataatctt
    12721 cgctttctgg tgagaaggtt tttttgccgc gccagaagcc cgggctggaa aaaagtaccg
    12781 tgtcatcgat atcaaacccc accgccattg gcggacgccc tgcgaggcta ttttcaattt
    12841 gtgcgaccga aacccaatga attggtgcct gttcagcaag cctggcaacg ttagtcccag
    12901 ggttaagcgg tgaaggagat gaggccaggg caacagcgga actgtttaga gcgaacaata
    12961 agcaaacggc actgattgcc tgtgtgatct tgcgcatatt tttccctaaa tagtcagtat
    13021 gttgaaactt tttattgtga gatttgttgc aaaaaccatc tgaccataac gccagctgcc
    13081 ggggaaagga aggattatct gcggtttttg tgatgaaaag caggatgatt atgtttaacc
    13141 cagtaaatac gttcctgtct ttttttgtat aaaaaatgtt tattactcga tgtaaggata
    13201 cattaatttt acgtttatta tgatgaatcc tctgcaaatg agcgaaattt gata
//
LOCUS       DAPHDW010000125        13193 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.127, whole genome shotgun sequence.
ACCESSION   DAPHDW010000125 DAPHDW010000000
VERSION     DAPHDW010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 13193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.127"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            99..1598
                     /gene="pitB"
                     /locus_tag="RH057_003508"
     CDS             99..1598
                     /gene="pitB"
                     /locus_tag="RH057_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HDV3129825.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPTDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRYWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(1647..2339)
                     /locus_tag="RH057_003509"
     CDS             complement(1647..2339)
                     /locus_tag="RH057_003509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV3129826.1"
                     /translation="MQSITPPLIAVIGSDGSGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANRPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            2513..3226
                     /locus_tag="RH057_003510"
     CDS             2513..3226
                     /locus_tag="RH057_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HDV3129827.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     ERIYLGQSSGRIGEWISQLPVIGAPFGRYLRSKAAHVHEKPSTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGFLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            3258..4016
                     /locus_tag="RH057_003511"
     CDS             3258..4016
                     /locus_tag="RH057_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129828.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERRLYQQMAQYRPELIIRLGIDIETAISRKPDHDYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQAQKAVSLVAIVSDRRSLT"
     gene            4062..5411
                     /locus_tag="RH057_003512"
     CDS             4062..5411
                     /locus_tag="RH057_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HDV3129829.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVVSALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVVAWYFDFGFAGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            5411..6235
                     /locus_tag="RH057_003513"
     CDS             5411..6235
                     /locus_tag="RH057_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129830.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVNRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYDAPLALDSDIRQRLELLISRFPL"
     gene            6247..6807
                     /locus_tag="RH057_003514"
     CDS             6247..6807
                     /locus_tag="RH057_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HDV3129831.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFIIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            6838..7908
                     /gene="lptF"
                     /locus_tag="RH057_003515"
     CDS             6838..7908
                     /gene="lptF"
                     /locus_tag="RH057_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HDV3129832.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYTQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRARSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLMLVARDFSLLQKFSR"
     gene            7905..8984
                     /gene="lptG"
                     /locus_tag="RH057_003516"
     CDS             7905..8984
                     /gene="lptG"
                     /locus_tag="RH057_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HDV3129833.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDDVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIKDDKTWILHGVNHKKWLNGKETL
                     ETSDNLAWQSAFTSMDLDELSMPGNTFSVRQLNHYIHYLQETGQPSSEYRLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(9020..10192)
                     /locus_tag="RH057_003517"
     CDS             complement(9020..10192)
                     /locus_tag="RH057_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV3129834.1"
                     /translation="MGLYDKYARLAGERLQFSDNGLTPFGTCIDEVYSATEGRIGNKK
                     VILAGTNNYLGLTFNHDAISEGQAALAAQGTGTTGSRMANGSYAPHLALEKEIAEFFN
                     RPTAIVFSTGYTANLGVISALADHNAVVLLDADSHASIYDACSLGGAEIIRFRHNDAK
                     DLERRMVRLGERAKEAIIIVEGIYSMLGDVAPLAEIVDIKRRLGGYLIVDEAHSFGVL
                     GATGRGLAEAVGVEDDVDIIVGTFSKSLASIGGFAVGSEAMEVLRYGSRPYIFTASPS
                     PSCIATVRSSLRTIATQPELRQKLMDNANHLYDGLQKLGYELSSHISPVVPVIIGSKE
                     EGLRIWRELISLGVYVNLILPPAAPAGITLLRCSVNAAHSREQIDAIIQAFATLKQ"
     gene            complement(10192..10440)
                     /locus_tag="RH057_003518"
     CDS             complement(10192..10440)
                     /locus_tag="RH057_003518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HDV3129835.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            complement(10472..11386)
                     /locus_tag="RH057_003519"
     CDS             complement(10472..11386)
                     /locus_tag="RH057_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HDV3129836.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASAVVHCAGQVRGHKEEIFTHCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYANSKHVAEQRLTAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVPGGYDW
                     QHVRQLAADARCGSVRMVGIPLPVLTCLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEQALRNGLF"
     gene            complement(11383..13113)
                     /locus_tag="RH057_003520"
     CDS             complement(11383..13113)
                     /locus_tag="RH057_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HDV3129837.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSSAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPG
                     DRCVSWLPFYHDMGLVGFLLTPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDDKTFM
                     PCYGLAENALAVSFSDEGSGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQASQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLHVQESLA"
BASE COUNT         3168 a         3269 c         3428 g         3328 t
ORIGIN      
        1 tctttttatc gttaaaaaat gatatttcac tttgcccatg ccgttaaagt cactgataat
       61 gcgtccgttc gtaaattcaa aatggcgtaa tctaatatat gctaaattta tttgttggcc
      121 ttgatatata cacagggctt ttgttattgc ttgctctggc atttgtgttg ttctacgaag
      181 caatcaatgg ttttcatgac acggcgaatg cggtggcaac cgttatttat actcgtgcca
      241 tgcaaccaca acttgctgtg gtgatggcgg cattttttaa cttttttggc gtgttattgg
      301 gcggacttag cgttgcctat gccattgtcc atatgttgcc aaccgatttg ttgctgaata
      361 tggggtcaac ccacggcctg gcgatggtct tttccatgct gctggcggcg attatctgga
      421 acctgggaac gtggttcttc ggtttaccgg cctccagttc gcacaccttg attggtgcga
      481 ttatcggcat cggtttaacc aacgcgctgt taaccggctc atcggtgatg gatgcgttaa
      541 acctgcgtga agtgaccaaa attttctcct cgctgattgt ttcccctatc gtcggcctgg
      601 tcattgcggg aggcctgata ttcctgctgc gacgctactg gagcgggacg aaaaagcgtg
      661 accgtattca ccgcattccg gaagatcgca aaaagaaaaa aggcaaacgt aaaccgccat
      721 tctggacgcg tattgcgctg attgtttccg ctgcgggcgt ggcgttttcg cacggcgcga
      781 acgacggaca aaaagggatc ggcctggtga tgctggtact ggtggggatt gcccctgctg
      841 gcttcgtcgt caatatgaac gcgtccggct atgaaattac ccgtacccgc gatgccgtca
      901 ccaacttcga acactacctg caacagcatc ctgaactgcc gcagaagttg attgcgatgg
      961 aacctccatt gcctgcagca tcgactgatg gcacgcaagt aacagagttt cactgtcatc
     1021 cggcaaatac ctttgatgcg attgcgcgcg ttaaaacgat gctgccaggc aatatggaaa
     1081 gttacgagcc gttaagcgtg agtcagcgca gccagctgcg ccgcattatg ctgtgcatct
     1141 ctgatacctc cgcgaagctg gcgaaactgc caggcgtcag taaagaagac cagaacctgc
     1201 tgaaaaaact gcgcagcgat atgttaagca ccattgagta cgctccggtg tggatcatca
     1261 tggcggtagc actggcgctc ggcattggca ccatgattgg ctggcgtcgt gtagcgatga
     1321 ccatcggtga gaagattggt aagcgcggca tgacgtatgc gcaaggcatg gcggcacaaa
     1381 tgacggcggc agtgtctatc ggccttgcca gttatattgg gatgcctgtc tccacaacac
     1441 acgtcctctc gtctgcggta gcagggacga tggtggtgga cggcggtggg ttacagcgta
     1501 aaacggtaac cagcatcctg atggcgtggg tgtttacttt accggcggca atttttcttt
     1561 ctggtgggct gtactggata gcattgcagt tgatttaacc ttcctgaaaa tgcccggtcc
     1621 tggcgatcgg gcatttccat ttttgactag tgataaccac gcgcggtcat aaaatccgta
     1681 atcgcttttt ctgcatcaac caacacctgt tccagtggcc gattggcatc gatatcaacc
     1741 agttgtgcac caccaaaggt taactgtggc gttatggcaa tcttcctcgc cagcgattcc
     1801 cgtttatggt cgggtttacg tgcacaggca acttcaaggt caacattgag tttgatgacc
     1861 agatcaggct tatggctcgc catccagtga aacgctttac gttcctggct tgccagccat
     1921 gagacaaaac gaccaccttc aacgttaggt gggaacaccg taccatcgta agcgccagga
     1981 atttggtcct gaggataacg gtcggttaga acaattaacc cgcgacgacg acaggcaagc
     2041 atatgacgaa agcgcagtaa gcgacgggcg acaaacgctg taattaccag cgccggaact
     2101 ggtccaggca atttttttgc tgttttcacc tgatttcgtt caattgtttt atgtaaggat
     2161 tttcccatca acggtaattt tgtcactgca cgaccgacat ttccggcctg ttttcctaaa
     2221 tgaactcttt cggcagcacc atatttttcg acaacggtaa tgagatgttc acacaccgtt
     2281 gacttgcctg aaccatcgct accaataacg gcaattaatg gaggtgttat tgactgcatg
     2341 aatatatcct taaatttaaa tatccatcca aaatatttat ttggttaata tgtttttatg
     2401 aaagcgtaat tcaggtcaat gtcacaatta accattgtca caataaggtt gaacggatat
     2461 tctgagcacg gaccacgtaa tattcaatac attatttact gccgggtttt tcgtgaatca
     2521 acgtaatatg tcaataatta attccacgcc agtgcgtgtt attgccattg taggatgtga
     2581 tggttcaggt aaatcgaccc tcacggcaag cctggtaaat gaactggcag caagaatgcc
     2641 aacagaacgc atttatctcg ggcaatcgtc cgggcgaatt ggcgaatgga tttcacagct
     2701 ccctgttatt ggcgcacctt ttgggcgtta tctgcgaagt aaagcggcac atgtgcacga
     2761 aaagccctca acaccgcctg gcaatattac tgcgctggtt atctatctgc tttcctgctg
     2821 gcgggcgtac aagtttcgca aaatgttgtg taaaagccag caaggctttc tgctcatcac
     2881 cgaccgctac ccgcaggttg aagtgccggg gtttcgcttt gatggcccgc aattggcaaa
     2941 aaccacgggc ggtaacggtt ggataaaaat gttaaggcag cgcgagctga agctgtacca
     3001 atggatggcg tcttatttgc ccgtattgtt gattcgtctt ggcattgatg aacaaaccgc
     3061 gtttgcgcgt aaacctgacc accaactggc agcgttacag gaaaaaatcg ccgttacgcc
     3121 gcaactgaca ttcaatggcg caaagatcct tgaactggat ggtcggcaac cagccgatga
     3181 aattatgcaa gcgtcactac gcgcaattca cgccgcgctt tcctgatcac caaaatggat
     3241 aggggatgat ttaacgaatg gatgcactac aaacacaaac tgttaatagt acaaccgcac
     3301 cgcagcccaa ctacattccg gggctgattg cggtggtcgg gtgtgatggc accggtaaat
     3361 ccacgctgac caccgacctg gtgaaatcgc tgcaacaaca ctggcaaacc gagcgccgct
     3421 atttggggct gctctccggc gaagacggcg acaaaatcaa acgattgccg ttggttggcg
     3481 tctggctgga acggcgactg gcggccaaat cctcgaaaac ccaaagcatg aaaaccaaat
     3541 ctccggcgct atgggcggcg gtgattatgt actgcttctc gctgcgaaga atggcgaatc
     3601 tgcgcaaggt tcagcgactg gcgcaaagtg gcgttctggt ggtcagcgat cgcttcccgc
     3661 aggctgaaat ttcgggcttt tattatgacg gaccggggat tggcgtcgaa cgtgcgaccg
     3721 ggaaaatcag catgtttctg gcgcagcgcg aacggcgttt ataccaacaa atggcgcaat
     3781 atcgcccgga attaattatt cgcctgggca ttgatattga gactgccatc tcccgcaagc
     3841 ctgaccatga ctatgccgag ctgcaggaca aaatcggcgt catgtcgacg attggctata
     3901 acggcacaaa aattcttgag attgattcca gagcgcccta cagcgaagtg ctggaacagg
     3961 cacagaaggc ggtttctctg gttgccatcg tttctgaccg ccgaagttta acttaaggct
     4021 ggcgggatac tttttactat caacgataag gtcaggctga attggcgggt tttaacatca
     4081 aacattggtt tgcagatggc gcgtttcgca ccattattcg caatagcgcc tggttaggct
     4141 ccagtaatgt cgtgagcgcc ttgctggggc tgttggcgct ctcgtgtgcc ggtaaaggga
     4201 tgacgcccgc catgtttggc gtactggtga ttgtgcaatc gtacgccaag tcgatcagcg
     4261 attttattaa gtttcagaca tggcagctgg tggttcagta cggaacgcca gccttgacca
     4321 acaataatcc gcagcaattt cgcaatgtcg tctcattttc cttctcgctg gatatcgtca
     4381 gcggcgcggt ggcgattgtc ggcggcattg ccttactgcc attcctttcc cattcattag
     4441 gtctggatga ccaaagcttt tggctggcgg cgctgtattg cacgctcatt ccttcaatgg
     4501 cttcctccac gccgaccggc attctgcgtg cggtagatcg cttcgattta attgctgtac
     4561 agcaggcgac gaaacctttt ctgcgcgcag cggggagcgt cgtagcctgg tattttgact
     4621 ttggttttgc gggttttgtt attgcctggt acgtgtcgaa tctggttggc ggcaccatgt
     4681 actggtggtt tgcagcgcgc gaattacgcc gcagaaatat ccataatgcc ttcaaattga
     4741 atctgtttga gtctgcccga cacatcaaag gcgcgtggag ttttgtctgg tcaaccaaca
     4801 ttgcccactc catctggtcg gcgcgtaact cgtgcagcac agtgctggtg gggatcgtgt
     4861 taggacccgc tgccgccggg ttatttaaaa tcgccatgac attcttcgac gccgccggaa
     4921 cgccagcggg tttgctgggt aaaagttttt acccagaggt gatgcgttta gatccgcgca
     4981 ccaccagacc gtggttgtta ggtgtgaagt ccggtttact ggcgggcgga atcggtattc
     5041 tggtggcgct cgcggtgttg atcgtcggca agccgctcat ttcgctggtg tttggcgtta
     5101 agtatctcga agcgtatgac ctgattcagg tgatgctggg cgcaattgtg atttcgatgc
     5161 tgggcttccc tcaggagtca ttgctattga tggcgggaaa acagcgcgct tttctcgtgg
     5221 cgcaaaccat cgcctcaatc ggctacatcg tattgctgtt catgttctgt catctgtttg
     5281 gtgtgctggg agcagcgttt gcttacttcg gtgggcaatg tctggacgtg gcgctctcgt
     5341 taattccaac cttaaaggca ttttttcagc gccattcctt gctttataac gcagccggag
     5401 agaaatcctg atgatgagaa aatatttccc gcttgaggcg agcgagcgtc tatttgttgc
     5461 gattgaagaa gatgatgtcg ttgatgcaca ggttagtttg ccgccgacta ttgcactaag
     5521 ttgtaccacc gaaatcattc atgataatta cgcgttatgc ctgcaattct ggctcaatgg
     5581 cgttaatcgt caggagttac tgcgtttagt tcgcaaacaa gccaaaggcg atgagctgac
     5641 tgctgatgag cgcaaacaat ttaaatacat gcgagcgcgc tacaagcacc tgcgttttgc
     5701 gcaacggctg tatttaaaga agcatcaggc cggattttta ttcggcaaaa cgaccgtttt
     5761 tctggggcgt tttcaggacg gctttcgtaa cggtaagaaa aatattgtgt cgtattacgg
     5821 caacctgtta cggatttatt taagctctcc ggtctggtcg ttggtgaact attcattacg
     5881 ccatagccag ttagaaagcg taagcagttt tattgcctat cggcaaaagc aaatgcatac
     5941 cctgaaagag attattgcca aaccgcgact aaccggcagg gaatttcatg atgtgcgaaa
     6001 aatcatcagc caacaggttt cttattacga caccttaaga tcgcttgatc ctgaaaataa
     6061 agaggcgtta cagatttccc gtttcctcgc cgccattaat ggcctgatgg gagataaaca
     6121 cgacgacatg gtggcggatg atatggaaaa ccgccagtcg tatgatgcac cgttggcgct
     6181 ggatagcgat attcgtcagc gcttagagct gctgatttcg cgttttccgc tatagccgga
     6241 gcaatgatgt catctcgttt gattattgcg ttgattatca tgctcttagc cccgggtgtg
     6301 caggcacaca atttcgtcac tggcaaaacg gttacccctg tttatatcca ggaaggcggc
     6361 gagctgcttc tgaatagcga cgacgaaatt cactaccaaa agtggaacag cacacaatta
     6421 gcaggaaaag tccgcattat tcagtatatc gccgggcgta agtcggcgaa gaaaaagaac
     6481 tcactgttaa ttaaagcggt tgaagcggca aactttccgc aggatcgttt tcagcccacc
     6541 accattgtga acaccgatga tgccatcttt ggtactggct attttgttgt tggcaaaatc
     6601 gaaaaaaata aacgccgcta tccgtgggcg caattcatta ttgacggcaa tggacaggga
     6661 cgcgttgcct ggcgcttgcc ggagcagagt tcaaccattc tggtgctgaa taaagccggg
     6721 caaattcagt gggcaaagga cggcagcctg acgccggaag aggtagatca tgtcattgcg
     6781 ctggcgcaga aactgatcaa tgaataaaaa attcaaagga ttgagggcgt tagtgagatg
     6841 aagctggttg aacactacat catgcgtggt acgcgccgtc tggtgctgat tatcgtcggt
     6901 tttctgatct ttattttcgc cagctactcc gcacagcgtt atctgaccga agcggcaaat
     6961 gggacattag ctcttgatgt tgtgctggat atcgttttct acaaagtgct gattgcactg
     7021 gagatgttgt tacctgttgg tctgtatgtg tcagttggcg tgacgctagg gcagatgtat
     7081 accgactcgg aaattaccgc tatctctgcg gcggggggca gtccgggacg cttgtacaaa
     7141 gccgttcttt atctggcgat accgctaagt atttttgtca cccttctgtc gatgtatggt
     7201 cgtccgtggg cttatacgca gatttatcaa ctggagcagc agtcgcagtc ggagctggat
     7261 gttcgccagt tgcgggcaaa gaaatttaac actaacgata acggacgaat gatcctttcg
     7321 cagacggttg atcaggataa taatcgcctg actgacgcgc tgatttatac ttctactgcc
     7381 aatcgaaccc gcattttccg cgcccgttcg gttgatgtgg ttgacccatc acccgagaaa
     7441 ccgaccgtta tgttgcacaa cggcactgcc tatcttctcg atcatcaggg gcgtgacgac
     7501 aacgaacaga tctaccgtaa tctgcaatta catctgaatc cgctggatca aagccctaac
     7561 gtcaaacgca aagcaaaatc ggtcacggag ctggcgcgct ccgcctttcc tgccgatcat
     7621 gccgaactgc aatggcgaca aagccgtggc ctgaccgcat tgttgatggc gctgctggcc
     7681 atttcattaa gtcgggtaaa accgcggcaa gggcgatttt caacgttatt gccactgacg
     7741 ttgctgttta ttgccatttt ttatggcggc gacgtctgcc gtacgctggt ggctaacggc
     7801 gcgattcctc tcattcctgg tttgtggtta gtgcccggac tcatgctaat gggcctgctg
     7861 atgctggtcg cacgcgactt ctctttgctg cagaaatttt cccgatgaat gttttcagtc
     7921 gctatttaat ccgtcatctc tttctcggtt ttgccgccgc cgcagggcta ttgctgccgc
     7981 tttttaccac cttcaacctg attaacgaac tggatgatgt cagcccgggc ggttatcgct
     8041 ggactcaggc ggtgctggtg gtgctaatga ccttaccgcg cacactggtc gaactttcgc
     8101 cgtttatcgc cttattggga gggattgttg gcctggggca gttatcgaaa aacagtgagc
     8161 ttaccgccat tcgcagcacg gggttttcta tcttccgtat tgcactggtg gcgctggttg
     8221 ccgggatatt gtggactgtt tcgttaggcg cgatagatga gtgggtggcg tcgccattgc
     8281 agcaacaggc gctgcaaatc aaatcgaccg ccaccgcgtt gggggaggac gatgacatta
     8341 ccggcaatat gctgtgggcc aggcgcggca atgaatttgt gacggtgaaa tcgctgaatg
     8401 aacagggcca gcctgtgggc gtggagattt ttcattatcg tgacgatctt tcgctcgaat
     8461 cctacattta tgcacgcagt gccaccatta aagacgacaa aacgtggatc ctgcatggtg
     8521 tgaatcataa aaaatggctt aacggtaaag aaacgctgga aacatcagat aatcttgcct
     8581 ggcaatcggc cttcaccagt atggatcttg atgagttatc gatgccgggg aatacttttt
     8641 ctgtccgtca gcttaatcat tacatccatt atttgcagga aaccgggcaa cccagcagcg
     8701 aataccgcct tgcactgtgg gaaaaactgg ggcaaccgat cctgaccctg gcgatgattt
     8761 tgctggctgt gccgtttacc tttagcgccc cgcgctcgcc agggatgggt agccgtctcg
     8821 ctgtaggcgt catcgttggc ttactcacct ggatcagcta tcaaatcatg gtcaatttgg
     8881 gattgctatt tgcgttaagc gcacctgtta ccgcgctcgg tttaccggta gcgtttgtgt
     8941 tggtggcgtt gagcctggtg tattggtatg acagacaaca ttaaacccgc cgtggcgcaa
     9001 acaccacggc gggctcacat tactgcttca gagtagcaaa tgcctgaatg atggcatcga
     9061 tttgctcacg actgtgggca gcatttacgc tacagcgcag cagcgtgatg cctgcaggcg
     9121 cggcaggcgg caggataaga ttcacataca cgcccagcga gataagctcg cgccagatgc
     9181 gtaaaccttc ctctttcgaa ccgattatta ctggcactac cgggctaata tgtgaactta
     9241 actcataacc cagtttttgc agaccgtcat acagatggtt ggcgttatcc atcagctttt
     9301 gccgcagctc tggttgtgtg gcaatggttc ttaacgatga gcgcacggtc gcaatgcagg
     9361 acggagaagg agaggcggta aaaatatacg ggcggctgcc gtagcgcagc acttccatcg
     9421 cttccgaacc gacagcaaaa ccgccaatcg aggccagact tttactgaaa gtaccaacga
     9481 taatatccac atcatcttca acgccgacag cttcagccag tccgcgcccc gttgcgccta
     9541 acacgccaaa cgaatgcgct tcatcgacaa tcaaataacc accgagacga cgtttgatat
     9601 caacaatttc cgccagcggc gcaacgtcac cgagcatgct gtagatgccc tcaacgatga
     9661 taatcgcctc tttcgcccgt tcaccgaggc gaaccatgcg ccgttccaga tctttagcat
     9721 cgttatggcg gaaacgaata atttccgcgc caccgagaga acaggcgtca taaatactgg
     9781 cgtggctgtc ggcatccagt aacaccacgg cgttatggtc ggcaagtgcg ctgatgacgc
     9841 ctaaattagc ggtataaccg gttgaaaaaa cgatagcggt agggcgattg aaaaactcgg
     9901 caatctcttt ctctaacgcc aggtgcggcg cataactgcc gtttgccata cgagaaccgg
     9961 tggtgccggt accttgggcc gctaacgcgg cctgaccttc gctgatggca tcatgattaa
    10021 aggttaaacc gagataatta ttggtcccgg ccagaatcac ttttttattg ccgatccggc
    10081 cttcggtggc ggaatacact tcatcaatac aggtgccaaa cggtgttaac ccgttgtcac
    10141 taaattgcag tcgctcgcca gcaaggcgcg catatttatc gtatagcccc attatttatt
    10201 ctccagccat ggaagtagag tttccattaa ttgcgcaggg gttttgacat ccagcagaat
    10261 attaatagga atggaaatat cgaatctgtc ttccagcatc attaagagat ccatcacttt
    10321 gatcgactcc aggccaagat cgttgacgag atcgctgtcg ggcttaattt ccacaccgtt
    10381 ttcgaccaaa tcctgtaaac aggaaaggat gtaatccatt actatttcac gatttaccat
    10441 agaaacgtta acacaccttt aaacaagaca atcaaaatag cccgttgcgt aatgcttgtt
    10501 ccaggctaat cccgggaaac caattaatat cttcagaaat acggttatta cttgccgacc
    10561 agtcggcgtg ggttaattca cgaattttcg agcgggtcag cataggttct ttacctgcca
    10621 ggcgactcaa cgcggtactg atatccgcaa ggcaggtgag taccggcaga ggaataccaa
    10681 ccattcgcac ggaaccacaa cgggcgtcgg cggcaagctg tcgtacgtgt tgccagtcat
    10741 agccgccggg gacgccatcg cataattcat aggtttgcgt ctgtacagtt tcggcgctta
    10801 accactgacc cactgcttgc gcgaaatcgg tgacgtgcag gaaagagagc tgtgtatctg
    10861 gcgcaccaag tcgtggcagc aggccgcgca gcatccagtc aaacagcggt tttaactctt
    10921 tatcgccggg accatacacg gctgtcgggc gaaaaacgcc cagcgtaatt tcgtcagcca
    10981 ttgcagtcag acgttgttcg gcgacgtgtt tggaatttgc gtaccaggag agctcgggat
    11041 ggcgcgccgc cagcgaagag ataaacagaa aacgttggca aaagccgctc tcttttgctg
    11101 cttgcatcag gcgcaggctg ccgtcaacgt tacagtgggt aaaaatctct tctttgtgtc
    11161 cgcgcacttg cccggcgcaa tggaccaccg cgctggctcc ggcaaccagt tcgctaagcg
    11221 aatgtgtatc ttccagcgaa ccgcgcaccc aagtaagatt atcattgacg tgagcgcggg
    11281 cagtacgcgt caatgcgcga acgtgaaagc cgcgggcgag caggttatcg ataatatatt
    11341 taccgataaa cccggtagcg cccgtcaccg cgacagtttg attcatgcca gggattcctg
    11401 cacatgaagg cttgcagcat aagccttctg ataacgtttt ttcgcttccg cacgggcagg
    11461 cttgccggag gacgttcggg gaatgctgtg gggcggcaac agatcgatag ccgctgttac
    11521 gccaaattcg ctttggatcc gtgctgccag cgcgtggata agctgtccgc gacgttcttc
    11581 atcgctgatc cgacactgga tctgcaaaat gattttttcc tgggcggtaa caaaagcaat
    11641 cgcatcgcca gaatgaattt ccggttcctg ttccgctata tattcaatat cctgcggcca
    11701 gatattacgg ccacgaataa taatcagatc tttaatgcgt ccggtgacat acagataacc
    11761 gtccagcaga taaccgaggt cgccggtgtc taaccagccc gtcgcggcaa tctcgtcttg
    11821 cgaagcctgg tcgccaaagt aaccgctcat cagactgggg ccggagatgc aaatatggcc
    11881 taccacacgt tccgcgaccg gcatacctgc ttcattgcgg atttcaatac catgttccgg
    11941 caacgctttg ccgcagttga cgaaagtcga tacggcgcgt gtctctgcac ccggcgcgac
    12001 ggctttaccc tgatattcga ggatgtcgcg atccacttcg ttaaccacaa ccccagagcc
    12061 ttcatcagag aagctgacag ccagcgcatt ttctgccagt ccgtagcacg gcatgaacgt
    12121 tttatcgtca aagttaacct gacggaaaca ttcagcgaat tgatggagtt gttctgcgga
    12181 gatcggctca gcaccaatac cagcgacgcg ccagcaggaa agatccagtt cagcgagatc
    12241 tttttcattc acgcggcgtt ggcacaattc atagccaaac ggcggcgcaa cggaaacggt
    12301 gccgcgattt ttactgatca atttaagcca ttgcaaagga cgcatggcaa aatcctgagt
    12361 gcgcaaataa tctactgaaa gttgcgtggc gacgggggtc aggagaaagc cgaccagtcc
    12421 catatcatgg tagaaaggca gccaggagac gcagcggtcg ccagggcgta atttaatgcc
    12481 atcgtggctt atagcacgta gattagccat cacttcgcga tgggtgataa tgacgccacg
    12541 gggaaaacgg gtgctgccgg aggtgtactg gaggtaggcg atatcgttcg gaactggacg
    12601 ctggagcgca acatcggctt ccggtaatgc cttaaaccag gcgtggctta aaacatgtaa
    12661 ttcggggttg ttatgcgtcg cggcattgac cagtggcaac cactcatcac cagtgataat
    12721 ggctgcgggc tggcaacttg ccagtaaacc ctggagtttg gcgctccagg aatcccgctg
    12781 accgacgccc attggaatcg ccaacgggac ggcgactaag ccagcatact gacaggcaaa
    12841 aaaagcctct acaaacccgc tactcgtttc ggcaatcagc gccacgcgtt cgcctttttt
    12901 caggttcagc gataacaacc gcttcgcacc agcttcggca cgcgttttta acgtctgata
    12961 ttccagttga tcttcaagtt ggcaacgtct gtcataaaaa ttcattccgg cgctactcag
    13021 agcggcgtag tccaaagcat caaccagcgt tggaaaatcg gcatagcgca taggtaggga
    13081 atgcgtaaag attttattag acatatacac cacgaaaaaa ttggatatca aaaataaaat
    13141 gacaataatg cggtggatta tataggcata aaagataaat gagatagtat ttt
//
LOCUS       DAPHDW010000126        12952 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.128, whole genome shotgun sequence.
ACCESSION   DAPHDW010000126 DAPHDW010000000
VERSION     DAPHDW010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12952
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.128"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            233..1342
                     /gene="livK"
                     /locus_tag="RH057_003521"
     CDS             233..1342
                     /gene="livK"
                     /locus_tag="RH057_003521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HDV3129838.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINAKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            1390..2316
                     /gene="livH"
                     /locus_tag="RH057_003522"
     CDS             1390..2316
                     /gene="livH"
                     /locus_tag="RH057_003522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HDV3129839.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIAGAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYGVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            2313..3590
                     /gene="livM"
                     /locus_tag="RH057_003523"
     CDS             2313..3590
                     /gene="livM"
                     /locus_tag="RH057_003523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HDV3129840.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDSASDVR
                     WQWVFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLAVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAILLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            3587..4354
                     /gene="livG"
                     /locus_tag="RH057_003524"
     CDS             3587..4354
                     /gene="livG"
                     /locus_tag="RH057_003524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HDV3129841.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            4356..5069
                     /gene="livF"
                     /locus_tag="RH057_003525"
     CDS             4356..5069
                     /gene="livF"
                     /locus_tag="RH057_003525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HDV3129842.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            5465..6781
                     /gene="ugpB"
                     /locus_tag="RH057_003526"
     CDS             5465..6781
                     /gene="ugpB"
                     /locus_tag="RH057_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HDV3129843.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAAFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTPVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYAAKLKASGMKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKNKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            6879..7766
                     /gene="ugpA"
                     /locus_tag="RH057_003527"
     CDS             6879..7766
                     /gene="ugpA"
                     /locus_tag="RH057_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HDV3129844.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSVVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            7763..8608
                     /gene="ugpE"
                     /locus_tag="RH057_003528"
     CDS             7763..8608
                     /gene="ugpE"
                     /locus_tag="RH057_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HDV3129845.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLQMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            8610..9680
                     /gene="ugpC"
                     /locus_tag="RH057_003529"
     CDS             8610..9680
                     /gene="ugpC"
                     /locus_tag="RH057_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol 3-phosphate ABC transporter ATP
                     binding protein UgpC"
                     /protein_id="HDV3129846.1"
                     /translation="MAGLKLQAVTKSWDGKTQVIKPLTLDVADGEFIVMVGPSGCGKS
                     TLLRMVAGLERVTEGDIWINDQRVTEMEPKDRGIAMVFQNYALYPHMSVEENMAWGLK
                     IRGMGKQQIAERVKEAARILELDGLLKRRPRELSGGQRQRVAMGRAIVRDPAVFLFDE
                     PLSNLDAKLRVQMRLELQQLHRRLKTTSLYVTHDQVEAMTLAQRVMVMNGGVAEQIGT
                     PVEVYEKPASLFVASFIGSPAMNLLTGRVNTEGTHFELDGGIVLPLNGGYRQYAGRKM
                     TLGIRPEHIALSSQAEGGVPLVMDTLEILGADNLAHGRWGEQKLVVRLAHQERPTAGS
                     TLWLHLAENQLHLFDGETGQRV"
     gene            9677..10420
                     /gene="ugpQ"
                     /locus_tag="RH057_003530"
     CDS             9677..10420
                     /gene="ugpQ"
                     /locus_tag="RH057_003530"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV3129847.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQLAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKVRVMQLKDAGLRILVYTVNKP
                     QRAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(10407..10847)
                     /locus_tag="RH057_003531"
     CDS             complement(10407..10847)
                     /locus_tag="RH057_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HDV3129848.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNGDMLNQPSTPQPDIPLKTIGP"
     gene            10967..12709
                     /gene="ggt"
                     /locus_tag="RH057_003532"
     CDS             10967..12709
                     /gene="ggt"
                     /locus_tag="RH057_003532"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HDV3129849.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDINKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            complement(12747..>12952)
                     /locus_tag="RH057_003533"
     CDS             complement(12747..>12952)
                     /locus_tag="RH057_003533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129850.1"
                     /translation="GRFSQGWYFCFEAREFLETGDEAARLAGNAPFIIDKDSGEIHSL
                     GTAKPLEEYLQDYEIKKATFGLP"
BASE COUNT         2862 a         3324 c         3714 g         3052 t
ORIGIN      
        1 cgttatttta tgctgacaaa ggcacttttt tctgtttatc tatcaataaa ttcagaatat
       61 tatctgttct taatcgactg aaaaataggg attttaatca ctattatcac aaaatactgc
      121 gctaaaccct taatcagaca ggcaaaaaca gtgcagtata aaaaaagaac agtctgattt
      181 gttaacacat aaaaacaaag caacacaaca tcacgaatgg ggatttttga ctatgaaacg
      241 gaatgcgaaa actatcatcg cagggatgat tgcactggca atttcacaca ccgctatggc
      301 tgacgatatt aaagtcgccg ttgtcggcgc gatgtccggc ccgattgccc agtggggcga
      361 tatggaattt aacggcgcgc gtcaggcgat taaagacatt aatgccaaag ggggaattaa
      421 aggcgataag ctggtgggcg tggaatatga cgacgcctgc gacccgaaac aagccgttgc
      481 ggtcgccaac aaaatcgtta acgacggcat taaatacgtt attggtcatc tgtgttcttc
      541 ttctacccaa cctgcatcag atatctacga agacgaaggt attctgatga tctcgccggg
      601 agcgaccaac ccggagctga cccaacgcgg ttatcaacac attatgcgta ctgccgggct
      661 ggactcttcc caggggccaa cggcggcaaa atacattctt gagacggtga agccccagcg
      721 catcgccatc attcacgaca aacaacagta tggcgaaggg ctggcgcgtt cggtgcagga
      781 cgggctgaaa gcggctaacg ccaacgtcgt cttcttcgac ggtattaccg ccggggagaa
      841 agatttctcc gcgctgatcg cccgcctgaa aaaagaaaac atcgacttcg tttactacgg
      901 cggttactac ccggaaatgg ggcagatgct gcgccaggcc cgttccgttg gcctgaaaac
      961 tcagtttatg gggccggaag gtgtgggtaa cgcatcattg tcgaatattg ccggtgatgc
     1021 tgccgaaggc atgttggtca ctatgccaaa acgctatgac caggatccgg caaaccaggg
     1081 catcgttgat gcgctgaaag cagacaagaa agatccgtcc gggccttatg tctggatcac
     1141 ctacgcggcg gtgcaatctc tggcgactgc ccttgagcgt actggcagcg atgagccgct
     1201 ggcgctggtg aaagatttaa aagctaacgg tgcaaacacc gtgattgggc cgctgaactg
     1261 ggatgaaaaa ggcgatctta agggatttga ttttggtgtc ttccagtggc acgccgacgg
     1321 ttcatccacg gcagccaagt gatcatccca ccgcccgtaa aatgcgggcg ggtttagaaa
     1381 ggttacctta tgtctgagca gtttttgtat ttcttgcagc agatgtttaa cggcgtcacg
     1441 ctgggcagta cctacgcgct gatagccatc ggctacacca tggtttacgg cattatcggc
     1501 atgatcaact tcgcccacgg cgaggtttat atgattggca gctacgtctc atttatgatc
     1561 atcgccgcgc tgatgatgat gggcattgat accggctggc tgctggtggc ggcgggattc
     1621 gtcggcgcaa tcgtcattgc cggcgcctac ggctggagta tcgaacgggt ggcttaccgc
     1681 ccggtgcgta attctaagcg cctgattgcg cttatttccg caatcggtat gtccatcttc
     1741 ctgcaaaact acgtcagcct gaccgaaggt tcgcgcgatg tggcgctgcc cagcctgttt
     1801 aacggtcagt gggtggtggg ccatagcgaa aacttctctg cctctattac caccatgcag
     1861 gcggtgatct ggattgtcac cttcctcgcc atgctggcgc tgacgatttt cattcgctat
     1921 tcccgtatgg ggcgcgcctg tcgtgcctgc gcggaagatc tgaaaatggc gagtctgctt
     1981 ggcattaaca ccgaccgggt gattgcgctg acctttgtga ttggcgcggc gatggcggcg
     2041 gtggcgggtg tgctgctcgg tcagttctac ggcgtaatta acccctacat cggctttatg
     2101 gccgggatga aagcctttac cgctgcggtg ctcggtggga ttggcagcat tccaggggcg
     2161 atgattggtg ggctgatcct ggggattgcg gaggcgctct cttctgccta tctgagtacg
     2221 gaatataagg atgtggtttc atttgccctg ctgattctgg tgctgctggt gatgccgacc
     2281 ggtattctgg gtcgcccgga ggtagagaaa gtatgaaacc gatgcatatt gcaatggcgc
     2341 tgctctctgc cgcgatgttt tttgtcctgg cgggcgtctt tatgggcgtg caactggagc
     2401 tggatggcac caaactggtg gtcgacagcg cttcggatgt ccgttggcag tgggtgttta
     2461 tcggcacggc ggtggtcttt ttcttccagc ttttgcgacc ggctttccag aaagggctaa
     2521 aaagcgtttc cggaccgaag tttattctgc ccgcgattga tggctccacg gtgaagcaga
     2581 aactattcct cgtggcgctg ctggtgcttg cggtggcgtg gccgtttatg gtgtcgcgtg
     2641 ggacggtgga tatcgccacc ctgaccatga tctacattat cctcggcctc gggctgaacg
     2701 tggttgttgg tctttctggt ctgctggtgc tggggtacgg cggcttttac gccatcggcg
     2761 cttacacttt tgcgctgctc aatcactatt acggcctggg cttctggacc tgtctgccga
     2821 ttgccgggtt aatggcagcg gcggccggct tcctgctcgg cttcccggtg cttcgcttgc
     2881 gcggcgacta tctggcgatc gttaccctcg gtttcggcga aattgtgcgc atattgctgc
     2941 tcaataacac cgaaattacc ggcggcccga acggaatcag tcagatcccg aaaccgacac
     3001 tcttcggact cgagttcagc cgtaccgctc gtgaaggcgg ctgggacacg ttcagtaatt
     3061 tctttggcct gaaatacgat ccctccgatc gtgtcatctt cctctacctg gtggcgttgc
     3121 tgctggtggt gctaagcctg tttgtcatta accgcctgct gcggatgccg ctggggcgtg
     3181 cgtgggaagc gttgcgtgaa gacgagatcg cctgccgttc gctgggctta agcccgcgtc
     3241 gtatcaagct gactgccttt accataagtg ccgcgtttgc cggttttgcc ggaacgctgt
     3301 ttgctgcgcg ccagggcttt gtcagcccgg aatccttcac ctttgctgaa tcggcgtttg
     3361 tactggcgat agtggtgctc ggcggtatgg gatcgcagtt tgcggtgatt ctggcggcaa
     3421 ttttgctggt ggtatcgcgc gaattgatgc gtgatttcaa cgaatacagc atgttaatgc
     3481 tcggtggttt gatggtgctg atgatgatct ggcgtccgca gggcttgctg cccatgacgc
     3541 gcccgcaact gaagctgaaa aacggcgcag cgaaaggaga gcaggcatga gtcagccatt
     3601 attatctgtt aacggtctga tgatgcgctt cggcggcttg ctggcggtga acaacgtcaa
     3661 tcttgaactg tacccgcagg agatcgtctc gttaatcggc cctaacggtg ccggaaaaac
     3721 cacggttttt aactgcctga ccggattcta caaacccacc ggcggcacta ttttactgcg
     3781 tgatcagcat ctggaaggtt taccggggca gcaaattgcc cgcatgggcg tggtgcgtac
     3841 cttccagcat gtgcgtctgt tccgtgaaat gacggtgatt gaaaacctgc tggtggcgca
     3901 gcatcaacaa ctgaaaaccg ggctgttctc tggcctgttg aaaacgcctt ctttccgtcg
     3961 cgcccagagc gaagcgctcg accgcgccgc cacctggctt gagcgcattg gtttgctgga
     4021 acacgccaac cgtcaggcga gtaacctggc ctatggtgac caacgccgtc tggagattgc
     4081 ccgctgcatg gtgacgcagc cggagatttt aatgctcgac gaaccggcgg caggtcttaa
     4141 cccaaaagaa accaaagagc tggatgagct gattgccgaa ctgcgcaatc atcacaacac
     4201 cactatcttg ttgattgaac acgatatgaa gctggtgatg gggatttcag accgaattta
     4261 tgtggtcaat caggggacgc cactggcaaa cggtacgccg gagcagatcc gtaataaccc
     4321 ggacgtgatc cgtgcctatt taggtgaggc ataagatgga aaaagtcatg ttgtcctttg
     4381 acaaagtcag cgcccactac ggcaaaatcc aggcgctgca tgaggtgagc ctgcatatca
     4441 atcagggcga aattgtcacc cttattggcg cgaatggcgc ggggaaaacc accttgctcg
     4501 gcacattatg cggcgacccg cgcgccacca gcgggcgaat tgtgtttgat gataaagaca
     4561 ttaccgactg gcagacggcg aaaatcatgc gcgaagcggt agcgattgtc ccggaagggc
     4621 gtcgcgtctt ctcgcggatg acggtggaag agaacctggc gatgggcggc ttctttgccg
     4681 aacgtgacca gtttcaggag cgcataaagt gggtgtatga gctgtttcca cgtcttcatg
     4741 agcgtcgtgt tcagcgggcg ggcaccatgt ccggcggtga acagcagatg ctggcgattg
     4801 gtcgcgcgct gatgagcaac ccgcgtttgt tgttgcttga tgagccatcg ctcggtcttg
     4861 cgccgattat catccagcaa attttcgaca ccatcgagca gctgcgcgag caggggatga
     4921 ctatcttcct cgtcgagcag aacgccaacc aggcgctaaa gctggcggat cgcggctacg
     4981 tgctggaaaa cggccatgtg gtgctctccg ataccggcga cgcgctgctg gcgaacgaag
     5041 cagtgagaag tgcgtattta ggcgggtaat aacacgttga ttgataagga gtcaaaagac
     5101 tcctttgaga caggtgacaa atgtaaaatt gcctgatgcg ctgcgcttat caggcctact
     5161 gggtgagttg caatatattg aatttgcacg atcttgtagg ccggataagg cgtttacgcc
     5221 gcatctggca tgaaacgatg agtaatctgt agagtttgat tcagaccttc tattttccgc
     5281 ttatccgtgc cccatctccc attttccctc acccacgccg tcaccgcctt gtcatctttc
     5341 tgacacctta ctatcttaca aatgtaacaa aaaagttatt tttctgtaat tcgagcatgt
     5401 catgttaccc cgcgagcata aaacgcgtga attcgcgcat tcggtacaac aagagagata
     5461 aacgatgaaa ccgttacatt atacagcttc agcactggcg ctcggactgg cgttaatggg
     5521 gaatgcacag gcagtgacga ccattccgtt ctggcattct atggaagggg aactgggtaa
     5581 agaggtggat tctctggccc aacgttttaa cgccgaaaat ccggattaca aaattgtacc
     5641 gacctataaa ggcaactacg aacagaattt aagcgcgggg attgccgcat ttcgtaccgg
     5701 caacgctccg gctattttgc aggtttatga agttggcacc gccaccatga tggcatcgaa
     5761 agccattaaa ccggtatatg acgtgtttaa agaggcgggg attcaattcg atgagtcgca
     5821 gtttgtgccg acggtttccg gttactactc cgacagcaaa acgggccact tactctccca
     5881 gccattcaac agctcgaccc ccgttctcta ttacaacaaa gacgccttca agaaagcagg
     5941 attagacccg gaacaaccgc cgaaaacctg gcaggatctg gcggactatg ccgcgaaact
     6001 gaaagcctcc ggtatgaagt gcggctacgc cagcggctgg cagggctgga tccaactgga
     6061 aaactttagc gcctggaacg gtctgccgtt tgccagcaaa aacaacggct ttgacggcac
     6121 ggacgcggtg ctggagttca acaagccgga gcaggtgaaa cacatcgcca tgctcgagga
     6181 gatgaacaag aagggcgact tcagctacgt cggtcgtaag gatgaatcca ccgagaagtt
     6241 ctataacggt gattgcgcga tgaccaccgc ctcttccggt tctctcgcta atattcgtga
     6301 gtacgccaaa tttaactatg gcgtaggcat gatgccttac gacgccgatg cgaaagatgc
     6361 accgcaaaac gccattatcg gcggggccag cctgtgggtg atgcagggca aaaataaaga
     6421 aacgtatacc ggcgtggcga agttcctcga cttcctcgcg aagccagaaa acgctgccga
     6481 gtggcatcag aaaaccggtt atctgccaat caccaaagca gcgtatgacc tgacccgtga
     6541 gcagggcttt tacgagaaaa acccaggggc ggataccgcg acgcgtcaga tgctgaataa
     6601 gccgccgttg ccgttcacca aagggctgcg tctgggcaac atgccgcaga tccgcgtgat
     6661 tgtggatgaa gagctggaga gcgtgtggac cggtaagaag acaccacagc aggcactgga
     6721 taccgccgtt gagcgtggaa atcagttgct gcgccgcttt gagaaatcga cgaagtctta
     6781 atcagtgtaa tgtcggatgc gtttcgctta tctgacctgg catcgcgtgt aggccggata
     6841 agcgaagcgc atccggcaca gttcaggaat taaccgtaat gtcatcatcc cgtccggtgt
     6901 tccgctcgcg ctggctgcct tatctgctgg tcgcgccgca gctcatcatc accgttatct
     6961 tttttatctg gcctgcgggc gaagcgttgt ggtactcgct acaaagcgtc gatccgtttg
     7021 gtttctccag ccagtttgtc ggcctggata acttcgtcac gctgttccat gacagctact
     7081 atctcgactc cttctggacg acgataaaat tcagcacctt tgtcaccgtc agcggtttgc
     7141 tggtatcgct gttctttgcg gcgctggtgg agtacatcgt gcgcggcagc cgtttctatc
     7201 aaaccttaat gttgctgcct tacgccgtgg ctcccgccgt tgccgccgta ttgtggatct
     7261 tcctgtttaa ccccggtcgc gggctgatca cccattttct cgcggagttc ggctacgact
     7321 ggaaccacgc gcaaaacagc ggtcaggcaa tgtttctggt ggtgtttgcc tcagtatgga
     7381 agcaaatcag ctacaacttc ctgttcttct atgccgcgct gcaatccatt ccccgttcgt
     7441 tgatcgaagc cgcagccatc gacggtgcag ggccgattcg ccgcttcttt aagattgcgc
     7501 tgccgcttat cgccccggtg agtttcttcc tgctggtagt gaacctggtg tatgccttct
     7561 tcgacacctt cccggtgatc gacgccgcca cgtccggcgg gccggttcag gccaccacga
     7621 cgctgattta taagatctac cgcgaaggtt ttaccggact ggatctggct tcgtctgccg
     7681 cacagtcggt ggtgttgatg ttcctcgtca tcgtgctgac ggtggtgcag ttccgctatg
     7741 ttgaaagcaa ggtgcgttac caatgattga gaaccgtccg tggctgacga tattcagcca
     7801 taccatgctg atcctcggga tcgcggtgat cctcttcccg ctgtacgtgg cgtttgtcgc
     7861 ggcgacgctg gataaacagg ccgtctatgc cgcgccgatg acgctcatcc ccggcacaca
     7921 tctgctggaa aacatccaca acatctgggt gaacggggta ggcacgaata gcgcgccgtt
     7981 ctggcggatg ttgcttaaca gctttgtgat ggcgttcagc attacgctcg gcaaaattac
     8041 cgtctcgatg ctctcggcat ttgccattgt ctggtttcgt tttccgctac gtaacctctt
     8101 cttctggatg atttttatca ccctgatgct gccggttgaa gtacgtatct tcccgacggt
     8161 ggaagtcatc gccaacctgc agatgctcga cagctacgcc ggtttaacgc tgccgctgat
     8221 ggcctcggcg accgctactt tcctgttccg ccagttcttt atgacgctgc cggatgagct
     8281 ggtggaagcc gcgcggatcg acggcgcatc gccaatgcgc ttcttttgcg acatcgtttt
     8341 tccgctctcc aaaactaatc tggcggcgct gtttgtgatc acctttatct acggctggaa
     8401 tcagtatttg tggccgttgt tgattattac cgatgtggat ctcggcacca ccgtggcagg
     8461 gatcaaaggg atgatcgcta caggcgaagg caccacggaa tggaactcag tgatggcggc
     8521 gatgttgtta acgcttatcc ctccggtggt gattgtttta gtgatgcagc gtgccttcgt
     8581 gcgcggcctg gtcgatagtg agaaataaga tggcaggact gaaattacag gcagtaacca
     8641 aaagctggga tggtaaaacc caggtcatta aaccgctgac ccttgatgtg gcggatggcg
     8701 aatttatcgt gatggtcggg ccgtctggct gcgggaaatc gacgctgctg cgcatggttg
     8761 ccgggctgga gcgggtgaca gaaggcgata tctggatcaa cgaccagcgc gtgactgaaa
     8821 tggagccaaa agatcgcggg attgcgatgg tgttccagaa ctacgcgctt tatccgcata
     8881 tgagtgtcga agaaaacatg gcgtgggggc tgaaaattcg cggcatgggc aagcagcaaa
     8941 ttgccgagcg cgttaaagaa gcggcgcgca ttctggagct ggacggtctg ctcaaacgtc
     9001 gcccgcgcga gctttccggc ggtcagcgcc agcgtgtggc gatgggccgc gcgattgtgc
     9061 gcgatccggc ggtgttcctg tttgatgagc cgctctctaa cctcgatgcc aagctgcgcg
     9121 tgcagatgcg tcttgaactg caacagttgc accgtcgcct gaaaacgact tcactctacg
     9181 ttactcacga tcaggttgaa gcgatgacgc tcgcccagcg agtaatggtg atgaacggcg
     9241 gcgttgccga acagattggc acaccagttg aagtctacga aaagcccgcc agcctgtttg
     9301 tagcgagttt tatcggcagt ccggcgatga acctgctgac aggccgcgtg aataccgaag
     9361 gcacgcattt cgaactggac ggcggtatcg tgctaccgct aaacggtggc taccgtcagt
     9421 atgccgggcg taaaatgact ctcggcattc gcccggaaca tattgcgcta agctcgcaag
     9481 cagaaggtgg cgtaccgctg gtgatggata cgctggagat cctcggcgca gataacctgg
     9541 cgcacggacg ctggggcgag cagaagctgg tggtgcgact ggcgcatcag gagcgcccga
     9601 cggcaggcag cacgctgtgg ctgcatctgg cggaaaatca gctgcatctt tttgatggtg
     9661 aaacaggaca acgagtatga gtaactggcc ttatccccgc atcgtcgctc atcgtggcgg
     9721 cggtaagctg gcccccgaaa acaccctggc ggcaatcgac gttggggcaa aatacggtca
     9781 taagatgatc gaatttgacg cgaagttatc gaaagatggc gagatcttcc tgctccatga
     9841 cgacaatctc gaacgcacca gcaacggctg gggcgtcgcg ggtgaactga actggcagga
     9901 tttactgcgc gtggatgcgg gcagttggta cagcaaagcg tttaaaggtg agccgctgcc
     9961 gttgctttcg caggtggcgg aacgctgtcg cgaacacggg atgatggcga atatcgaaat
    10021 caaacccacc actggcactg gaccattaac gggcaaaatg gtggcgctgg cggcacgcca
    10081 actgtgggcc ggtatgacgc cgccgctgct gtcatcgttt gagattgatg ctttagaagc
    10141 tgcacagctg gcggcaccgg aactgccgcg tggtttattg cttgatgagt ggcgcgacga
    10201 ctggcgcgaa ctgaccgcgc ggctgggctg cgtctctatt catctcaatc ataagttact
    10261 cgataaagtg cgagtgatgc agttgaaaga cgccggactg cggattctgg tttataccgt
    10321 caataaaccc cagcgcgcgg cagagttgct gcgctggggc gtggattgca tctgtaccga
    10381 tgcgattgac gtgattggtc cgaactttac ggcccaatag ttttcaacgg aatgtcaggc
    10441 tgcggcgtgc tgggctgatt cagcatgtcg ccgtttctcc tctccggcag catatgctgc
    10501 tgattcaacg aactatccgg attacggttg ctgtttaaca tcccgccgtt ggtgttgggc
    10561 agcatttgct gccgggcggg atttcgctcc cccggctgtg actgcaacac ccgctgagaa
    10621 ttattgttta tctggttttc taaatgctgc tgttgcaact gagtttgcgt tttcagttgc
    10681 tgattcagca tccctttttg ctggatttgc tgagtctgca tctgggtttg catccgctgc
    10741 tggctgggta tctgataccc cggctggtta gggttgttca gagtattaat gggctgtgca
    10801 aagccgacaa acggcaggag tgccgtaaga atcagaagtc gtttcatcgc gtatcctcct
    10861 ctgaagatat cctttaagtt tactcgcttc ccgacaaaac gatgattaat tcagagttat
    10921 ataccaggct tagctggggt tgccccttaa tctctggaga ataacgatga taaaaccgac
    10981 gtttttacgc cgggtggcca ttgctgctct gctctcagga agttgtttta gcgccgccgc
    11041 cgcgcctcct gcgccgcccg tctcgtatgg tgtggaggaa gatgtcttcc acccggtacg
    11101 cgcgaaacag ggaatggtag cgtctgtgga cgccactgcc actcaggtgg gggtggatat
    11161 tctcaaggag ggcgggaatg ccgttgatgc cgccgtggcg gtgggctacg cgctggcggt
    11221 aacgcatccg caggcaggga atctgggcgg tggtggtttt atgttaatcc gctcgaaaaa
    11281 tggcaatacc acggctatcg atttccgcga aatggcaccc gccaaagcga cccgcgatat
    11341 gttcctcgat gatcagggca acccggacag caaaaaatca ctcacttcgc atctggcttc
    11401 cggcacaccg ggtacggtag caggtttctc gctggcgctg gataaatacg gcaccatgcc
    11461 gctgaacaaa gtcgtgcagc ccgcgtttaa actggcacgc gatggtttta tcgttaacga
    11521 cgcgctggct gacgatctca aaacctacgg tagcgaagtg ttgccgaatc acgaaaacag
    11581 taaagctatc ttctggaaag agggcgagcc gctgaaaaag ggcgacacgc tggtgcaggc
    11641 gaacctggca aagagcctgg agatgattgc tgaaaacggc ccggacgaat tctataaagg
    11701 cacgattgcg gaacagatcg cccaggagat gcagaaaaac ggtggcttga tcactaaaga
    11761 agatttagca gcctataaag cggtcgaacg cactccgata agcggcgatt atcgcgggta
    11821 tcaggtttac tccatgccac cgccatcctc cggcgggatc catatcgtac aaatcctcaa
    11881 tattctggaa aacttcgata tgaagaaata cggctttggc agcgccgatg cgatgcaaat
    11941 catggcagaa gcggagaaat acgcctacgc cgaccgctcg gaatatcttg gcgacccgga
    12001 ttttgtcaaa gtaccgtggc aggcgctgac caataaagcc tatgccaaat ctattgccga
    12061 tcaaattgat atcaataaag cgaagccatc cagcgaaatt cgccccggca agcttgcgcc
    12121 ttatgagagt aatcaaacta cccattactc agtggtggat aaagatggta acgcggtggc
    12181 ggtgacctat acgctgaaca ccaccttcgg tacgggcatt gtcgcgggcg agagcggtat
    12241 tctgcttaat aaccagatgg atgatttctc cgccaaaccg ggcgtaccga acgtttacgg
    12301 gctggtgggc ggtgatgcca acgccgtcgg gccgaacaaa cgcccgctgt cgtcgatgtc
    12361 gccgaccatt gtggtgaaag acggtaaaac ctggctggtt accggtagcc caggcggtag
    12421 ccggatcatc actacagtgc tgcaaatggt ggtgaatagc atcgattatg gcatgaacgt
    12481 cgccgaagcg accaatgcgc cgcgtttcca ccatcagtgg ttgccggacg agctgcgtgt
    12541 cgaaaaaggg tttagcccgg atacgctcaa gctgctggaa gcaaaaggtc agaaagtggc
    12601 gctgaaagag gcgatgggca gtacacaaag cattatggtt gggccggacg gtgagttgta
    12661 cggcgcatcc gacccgcgct cggtggatga tttaacggcg gggtactaag gttagcggcc
    12721 ctcttcgtgg gaagagggct attttgtcag ggcaagccga aggtagcctt ttttatttcg
    12781 taatcctgta gatattcttc cagcggcttt gccgttccca gagaatgaat ttcaccactg
    12841 tctttatcaa taataaaagg tgcgttacca gctaagcgcg cggcctcatc tccagtttcg
    12901 agaaattctc gtgcttcgaa acagaaatac cagccctggc taaagcgtcc at
//
LOCUS       DAPHDW010000127        12835 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.129, whole genome shotgun sequence.
ACCESSION   DAPHDW010000127 DAPHDW010000000
VERSION     DAPHDW010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.129"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..872)
                     /gene="yhaJ"
                     /locus_tag="RH057_003534"
     CDS             complement(<1..872)
                     /gene="yhaJ"
                     /locus_tag="RH057_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HDV3129851.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLRE"
     gene            977..1678
                     /locus_tag="RH057_003535"
     CDS             977..1678
                     /locus_tag="RH057_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HDV3129852.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDACPQRENPLIQKLALNMGKQQLIASPEGTVGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            1701..1865
                     /locus_tag="RH057_003536"
     CDS             1701..1865
                     /locus_tag="RH057_003536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129853.1"
                     /translation="MSKKLAKKRQPVKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(2077..3387)
                     /locus_tag="RH057_003537"
     CDS             complement(2077..3387)
                     /locus_tag="RH057_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HDV3129854.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AIADAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTQQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITATMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(3415..4746)
                     /locus_tag="RH057_003538"
     CDS             complement(3415..4746)
                     /locus_tag="RH057_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026203.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV3129855.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTGMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSPFLAFS"
     gene            complement(5021..6385)
                     /gene="tdcG"
                     /locus_tag="RH057_003539"
     CDS             complement(5021..6385)
                     /gene="tdcG"
                     /locus_tag="RH057_003539"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV3129856.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPRRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(6457..6846)
                     /locus_tag="RH057_003540"
     CDS             complement(6457..6846)
                     /locus_tag="RH057_003540"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HDV3129857.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(6860..9154)
                     /gene="tdcE"
                     /locus_tag="RH057_003541"
     CDS             complement(6860..9154)
                     /gene="tdcE"
                     /locus_tag="RH057_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HDV3129858.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKNEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(9188..10396)
                     /gene="tdcD"
                     /locus_tag="RH057_003542"
     CDS             complement(9188..10396)
                     /gene="tdcD"
                     /locus_tag="RH057_003542"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HDV3129859.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSCGERIVSSENARVICAVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(10422..11753)
                     /gene="tdcC"
                     /locus_tag="RH057_003543"
     CDS             complement(10422..11753)
                     /gene="tdcC"
                     /locus_tag="RH057_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HDV3129860.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTISMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(11775..12764)
                     /gene="tdcB"
                     /locus_tag="RH057_003544"
     CDS             complement(11775..12764)
                     /gene="tdcB"
                     /locus_tag="RH057_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HDV3129861.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
BASE COUNT         3274 a         3528 c         3067 g         2966 t
ORIGIN      
        1 atttcccgca gacaccagga tttcgcttcc cccatactgt cacgacgcca ggccataata
       61 atatcgatct cgctggtcga ttccgggctg acgacacgca accgcccttc cgcaatatct
      121 ttttcgacca tcggatacgg catcgtcgcc acgccaagcc ccgccagtaa tgcctgacgt
      181 ttatcttcaa tcgtgctcac cgttaagcgc ggctgtttgt ccagcagctg tacggtcaac
      241 accgggcgct cacgagcggt atccgctacc gcaattccac gatatttcac gcgcgtcact
      301 tcagataacg gttccggctc ctgatgaatc gggtgatcag gcgcggcaac gtagacgttc
      361 attaacgtat agagcttgcg cgagttgatc tccgacgagg aacgaaaatg catatccggc
      421 gcgataacaa tatccgcccg cccctgctcc agtcgttccc acgcccccgc cagcacttcg
      481 gtgatgattg ccagttgggt attggctttt gccgccagtt tgtcgattaa cgggaaaaag
      541 gcaggtgtcg gtaccagcgc ttcggtcaca atggtgagat gcgtttccca accgcgcgcg
      601 agggcttccg catcggtagt cagtttatct gcggcttcca gcaaaacgcg ccctcgctcc
      661 agcaacatcc tcccgacatt ggtgaatttg gtgcgatggc ccgagcggtc aaacagcacc
      721 acatccagct cttcttccag tttttgcatg gtgtagctaa gtgcggaagg cacgcgtccc
      781 agctcatccg ccgccgccgc aaaactgccc cggcgatcga tcgcatccat aacccgtagt
      841 gcttccagcg ttaatgccct ttctttggcc atttcgttct cattcaggaa atttgaacat
      901 accgggcaga atatctggct aacaatgtag cgtccagccc cttaccataa aaggaagtaa
      961 agagaggtca agaattatga ttactacccg aactgccagg cagtgtggac aagcagacta
     1021 cggatggttg caggcccggt atactttttc ctttggacac tacttcgacc cgaaattgtt
     1081 aggctatgcc tccctgcgtg tgcttaacca ggaagtgctg gccccaggtg ccgcctttca
     1141 gccgcgaacc tatcccaaag tcgatatttt aaatgtgatt ctggatggag aagcagagta
     1201 tcgcgacagc gaaggcaatc atgttcaggc cagcgccggt gaggcgttgc tgctctctac
     1261 ccagccgggt gtcagctaca gcgaacacaa tctcagcaaa gacaaaccgt taacacgaat
     1321 gcagctttgg ctggacgcct gcccgcagcg agagaatccg ctgattcaaa aactggcgct
     1381 taatatgggc aagcagcaat taatcgcctc gccagagggc acagtgggta gcctgcaact
     1441 gcgccagcaa gtgtggctgc accatatcgt gctcgacaaa ggcgaaagtg cgaatttcca
     1501 gttgcatggg ccacgcgcgt atttgcaatc gattcacggg aaatttcatg cgcttacgca
     1561 tcatgaagag aaagcggcgc taacctgcgg tgatggggcg tttattcgtg acgaggctaa
     1621 cattacgctg gttgccgatt ccccactgcg cgctttgctg atagatttgc ctgtctagtt
     1681 gtttttacag ggagatgatg atgagtaaaa aattggccaa aaagcgccag ccggtgaagc
     1741 ccgtggtggc gaaagaacct gctcgcaccg ccaaaaattt tggctatgaa gagatgttga
     1801 gcgagctgga agctatcgtc gcggatgctg aaacgcgttt agccgaggat gaagctaccg
     1861 cgtaaagaca ggttgccgga tgcggcgaaa acgccttatc cggcctacaa aatccaacaa
     1921 cattaacgaa ttaaacaacc cgtaggccgg ataagatgcg ccagcatcgc atccggcatc
     1981 aggtacaaaa cacaacaaca tcaacaagtt gagcacccct taggccggat aagatgcgcc
     2041 agcatcgcat ccggcacgat acccaaaacc tggcgtttat ctggccttgc tcgccataat
     2101 ctcgataatc tgccgatccg tttgctgcat cgaatggctt gctaacgcac acaggttggc
     2161 aatcgactgc tcaacatcat gcgccacgat cccttcattg ccggtcacgg cggtatcatc
     2221 cagcgccatt aacaccgctt tccacgcagc cgaagcactg gtcgaaacct tcatcgcaca
     2281 gctgttcgac gctccatcgc aaatcatgcc gctgacatcg ccgatcatac tgctgatcgc
     2341 catcgagata gtttcataac gcccatccac cagccatgcc atcccggcgg cggcccccat
     2401 tgctgcggtc gttgcggcac aaagtgcaga caaacgcggt aactggttat ggatgtaaat
     2461 tgcgctcaaa tgcgaaagca tcagcgcacg cgccagccgt tcatcatccg ctccgaagtg
     2521 ttctgctacc accaccacag gcattgttgc ggtgatcccc tggttacccg agccagagtt
     2581 actcattgcc ggaagcgtag cgccgcccat acgcgcatcg gatgccgcgc tggtacgaat
     2641 cacaatggat gaagagagat ctttcgccag caaaccgcgc tcgcactgtt tttccagcgt
     2701 cgcgccaata tgcagccccc acttaccgct caaaccttcc tgcgataacg cacaatttag
     2761 cttcgcggaa tcgagaataa agcggatcgc cgcaaacggg acttcattga cgaacttcag
     2821 gatctcagcc agcgtcgttc tggaaagcac cgtcagcgga gactcttgct cgccctctgc
     2881 cacacacgcc tgctgggtaa acaccacacc attgtgcgtc tcaatatgca caatgttggt
     2941 atgcccgccg acgatggtga cacacgccca cttctcaccg ttccagactt tggcgcgtga
     3001 gaagaggatt tcattgcaag gttcctggat cttaacggag actttccccg ccgccagcag
     3061 tgctttggca tcggcaattg cctgcgcagt tgcgtctttc agcacttcca gcccggcgtt
     3121 ggcatttcca cctaacgccc ccagcgccgc cgcaatcggc agccccacca ttcccgtgcc
     3181 gggaacggtg acgcccagac cgttcttcat cagatttggc gaaacccagg cttctacacg
     3241 ttcaaccgga ccttccagtt ctgccgcagc aaccgccgcc gccagcgcca gtgaaatcgg
     3301 ttcagtacat cccagcgccg gttttacttc ctcctgaacg gcgaggatgt aacgctgcca
     3361 taacggattt aaagtcgaat caaacatatt aaaaacctta aaatttcagg taaatcaaga
     3421 aaatgccagg aacggagaaa cacaaagcaa caaaccagtg acgataatca ggtacagaga
     3481 catccctttg tatttgtgca atgccggtac tttgtaaacc agccacgccg ggatcaggca
     3541 ccctaccatg ccgaaaatcg ggctacagat agaggtgaag ctcaacaccg gtgcgttcag
     3601 tacgatggcg ctccaggcca gcaaaatggc gaaaatcatg atgccgcgct gaacaagatt
     3661 ttcgttaatc ttctcggcag gcatcttgcg acgcaggatg ttcattacga tcccttgcgt
     3721 tgcttcgcga aagcctaaat agacgccaaa gaatgcagtc atgacagcaa agatattgag
     3781 aatgacgctg accactttca cccacgctgc gccgtcaccg ctaataaact gtgcggcaat
     3841 cgccagcgcg gaaatattct gctcataggc ttttaccgct tcgtcatgtc ccatcgccag
     3901 cgtgaacgac acggcgtaga aaaagacggt gacaaacaaa atgccaaacg cgatattcat
     3961 tgcccgcaat gctttatgcc gcgccacttc aattgatttt tcccgcgagc gataagagat
     4021 caccatcggg cttaacgtct ggataaacag aatcgacgtc agggtaaacg gcagcgtaat
     4081 aatggcgttt ttcaccagca gccccagcgg cggtagcgaa ccgacgttat acagatgcca
     4141 cattcccacc atcgacacgc ccagcgccgc gaccaccagc agcttggtca gcaccatgcc
     4201 ggtcgaaatt ttgaataaca atttctcgcc gcgtgaagag atcgccacca gaatgcaaat
     4261 cagtaccaga ccataaaagg gactgtctga cagcaaccct tccgtcacgc cgaaggtatg
     4321 cagataggaa gcactatcgt tggtgatggc ggtggaatag acgaacatcc aaatcaccag
     4381 cattacgaaa tagagcgcac ctaacaggat gccccagttt ttacctaaat aaccgctaat
     4441 gacactcggg taatctttac attctggtga ttctgccagc gtattaataa acaaccgctg
     4501 aaacagatac attgccgggt aaccaatcac cgatgagagc aaaaataccc acaatcccat
     4561 caaaccgacc tgcaccggga gaaaaacaat ccccgcgcca atcgccatcc cgatactcat
     4621 aatcacccag ccggtgtcag tactgtcgaa tttgatcgct tctcgccact cgctctcact
     4681 cattccagca cgacccgccg gggtcgaagc gtctgcaatg acgcctttat tcgatgcaat
     4741 ttccataatt tctcgctcaa tattttgtag ggcttattat ttttttccga gccgcatcaa
     4801 ggcgatatgc ggtctacgtt tttgcaggcg agttattaga ataaagaaat gatacgcgcg
     4861 aggtcagaga aaatcttcac aatcaagact ttgaaataac ggagatggat aagaattttc
     4921 tactaaatta atcgcaagag aatattcatt ctctatttgc ggcagatcac aaaaaaaagg
     4981 tgcacatttg tgcacccaag gatgaaagct gacagcaatg tcagccgcag accactttaa
     5041 tggccagtcc tccgcgtgat gtttcgcggt atttatcgtt catatctttg ccggtttcat
     5101 acatcgtctc gatcacttta tcgagtgaaa cacgcggtgc cgaggtgcgg cgcatcgcca
     5161 tccgcgcggc gtttactgct ttcacggcat taatggcatt acgttcaatg cacgggattt
     5221 gtacctgtcc ggcaaccgga tcgcaggtca gcccaaggtt atgctccatc gcgatttccg
     5281 ccgcattgca tacctgcgcc ggactgccgc ccagtagttc agttaaccct gccgccgcca
     5341 ttgaacaggc cacgccaatc tccccctgac agccgacctc cgcgccagag atggaggcgt
     5401 tcattttata cagcgcgcca atagcccccg ctgccagaaa atagcgggca attgaccgct
     5461 cgtttaccgg acgacggaac ttatcgtaat aagccagcac tgccggaata atgccgcacg
     5521 caccgttagt cggtgccgtt accacgcgcc cgccagctgc gttttcttca ctaaccgcca
     5581 gcgcgtacat gttgatccag tcgatgacat tcatcggatc gttagagatg ttatcgctgg
     5641 aaaccagctg acgacgcagc gctacggcac gacgcggcac attgagcgga ccaggcagca
     5701 cgccttcagt gttcatcccg cgttcaatac cgtcatgcat cacttgccag atacgggcaa
     5761 accctgcgtc aatttccgct ttgctgcgca gcgccagctc gttgtgcatc atcaggccag
     5821 atatagacag gccgttgtaa tcacacattt tcagcagttc acctgctgag tggaaatcgt
     5881 aaggtacgga cgtttcgaca tcgtgcgaca ggccgaagtg ttcttcttcg acaataaacc
     5941 cgccgccgac agagtaatag gttttactta atagcgcttc ctgtcccttc caggcagtga
     6001 tccgcattcc gttctcatgg cgaggcaaca tttcgggatg gaagataatg ttctttgcta
     6061 caggaaaatc aacaatatgc gcaccagatg ccactggcag ccgcccgctg cgcgttacta
     6121 actctataaa tgcagggatc tcatcaatga caacatcctg cggactgttt cctgccagtc
     6181 ccatgataat ggcgacatcc gtggcatggc ctttgcccgt cagtgacaac gacccataca
     6241 gatcgaccac aatatggctc gtcgcggtta ataagccgct actttccagc cgatcaataa
     6301 aactttttcc ggcattcatt ggccccacgg tatgcgaact ggagggacca atcccaattt
     6361 tgaaaatatc gaatgcacta atcatatcca caccctcgga ttgccgttca gtgaagtgga
     6421 gcggaacgac cttgcgaccg tcccgctcac gaggctttac gcactacgta ctgcgatagc
     6481 ttcaatttcc agcttcacat ctttcggcaa acgcgcaacc tgcacacagc tccgggtcgg
     6541 gtaggtcgcc tgatgctcat cgaagaactg cttatagact tcattgatgg tggcaaaatc
     6601 attcagatcg gtgatgaaca cggtcatctt gatgatatcg cccacgctca gcccggcagc
     6661 aaccacgatc gctttgacgt tttcgaggct taaacgcgcc tgatcttgca catcagccgg
     6721 gatctcaccg gtctgcgggc aaaccggaat ttgcccggag gtgaagacca tgctgcctaa
     6781 atcaacgccc tgaacataag ggccgattgc gcctggggca cgttgcgttt cgataatctt
     6841 tttcatacat cctccggcgt cagagcgcct gggtaaaagt acgtgaaata acatcctgtt
     6901 gctgttcacg ggtcagtgcg ttgaagcgca cggcgtagcc agagacacgg attgtcaggt
     6961 taggatattt ttccgggtgc tcgatggcat ccagcagcat ttcccgattc attacgttga
     7021 cgttgaggtg ttgaccgcct tcgacatccg cttcgtggtg gaaataccca tccagcaggc
     7081 cgacaaggtt ggttttacgt actggatctt ctttgcccag cgccgcagga acgattgaga
     7141 aggtgtacga gatcccatct ttggcgtagg tgaacggcag tttcgccacc gacgtcaatg
     7201 aggccacggc acctttgcgg tcacgaccat gcatcgggtt agcgcccggc gcgaacggtg
     7261 ttccggcgcg acgaccgtcc ggcgtattac cggttttctg gccgtacacc acgttagaag
     7321 tgatagtcag aatcgactgg gtagggacgg cgttgcgata ggttggcagc gctttaattt
     7381 tcttcataaa gcgttcaacc aggtcgcagg caatgctgtc tacgcgctcg tcgttgttgc
     7441 cgtactgcgg atattcaccg tcgatttcaa agtccaccgc caggccgttt tcgtcacgga
     7501 ttggtttcac gcgggcatat ttgatggcag acagggagtc cgtcgccacc gacaggcccg
     7561 cgatgccgca tgccatagtg cgatagacat cacgatcgtg cagcgccatt agcgaagctt
     7621 cgtagctgta cttgtcgtgc atgtagtgaa tgatattcag cgcgctgatg tactgcaccg
     7681 ccagccagtc catgaagtga tcgaggctgt ccatcacttt gtcgtaatcc agcacgtcgt
     7741 ccatcagcgg tgctgttttc ggcccgacct gaatcttcag cttctcgtcc accccgccgt
     7801 taattgcgta gagcagcgtt ttcgccaggt tagcgcgtgc accaaagaac tgcatttgct
     7861 taccaatcac cattgggctg acgcagcagg caatcgcgta atcgtcgctg ttgaagtcag
     7921 tacgcatcag atcgtcattt tcatactgca aggaagaggt gacgatcgac acctgcgcgg
     7981 catatttttt gaaggcaatc ggtaattctt ccgaccaaag aatggtcagg ttaggttccg
     8041 gtgccggccc catagtgtgc agggtgtgca aatagcggaa ggagtttttg gtcaccagcg
     8101 tacgaccgtc cagccccatc ccgccgatca cttccgtcgc ccagattggg tcgccggaga
     8161 acagcgaatc aaattccggt gtacgcagga agcgtaccat acggatcttc atgatgaagt
     8221 gatcgatcag ttcctgtgcc tgctgctcat tgagtacgcc agctttaaag tcgcgctcaa
     8281 tgtagatatc gaggaacgat gccgtgcggc ccagcgacat cgcgccgcca ttttgcgatt
     8341 tcactgccgc cagataagcg aagtagagcc actgcaccgc ttcctgcgca ttctgcgccg
     8401 ggcgagagat atcaaagcca tatttcgccg ccatttcctg aatctgcaac agcgcatgac
     8461 gatgctctgc cagctcctca cgcagacgga tggtggcttc cagatcctcg cctttttcca
     8521 gacgagactg gagatcggca aattgcagtt cgcgttcacg taccagataa ctgatgccat
     8581 acagcgctac gcggcgatag tcaccgataa tgcgcccacg gccatagcca tctggtaaac
     8641 cggtcagcac gccagattta cggcagcgca gcatatccgg tgagtaaaca tcaaatacgc
     8701 cctggttatg ggttttacgc agatcggtaa acagatattc aaattcactg tccatttcac
     8761 ggccataggc gtggaatgaa cttttaatca tattaatgcc accgaacggg tgtagcgcac
     8821 gtttcaacgg cgcatccgtt tgcaggccaa caattttttc cagcggctgg ttaatatatc
     8881 ccgcatcatg agcggtaatt gtggtggcaa tattggtatc gaaatcaacc ggcgcgtggg
     8941 ttgcgttttc gatacggata ccttccatta ctttttccca caattccgtg gtggcaggcg
     9001 tcgcttcggc gaggaaagat tcatcgcctt catacggtgt atagttatgt tgaataaaat
     9061 cgcggacatt aatttcgttt ttccagtccg tacctttaaa gccaagccat gcgtcggcgt
     9121 acagcttatc gctggtatca atatctacct tcatgaaaaa taatctctct acaatacttc
     9181 aactaaatta tgcaaattct gcgggcgcgt taactttgcc taaatgaatg gcatccaaag
     9241 caatcatttt ttcttcgtta gtcggaataa cggcacaaat gacacgcgca ttttcactgg
     9301 aaacaattcg ctcaccacag gagttagagc gattattcat ttctgtatca atctctacgc
     9361 ctaatacagc caaatgttcc atgaccagac gacgaattaa gcttgaattc tctcctattc
     9421 cgccggtgaa tataattcca tccaggcgac gtaatgaagc tgcgtgtccg gcaatatgac
     9481 gggcaattcg gtgaacaaag gttttaattg ccagttgcgc gcgttcgtga ccttcatgcc
     9541 aggctttttc cagaacacgt aaatccgaag aaagaccgga aatacctaat aatcccgact
     9601 ctttattcac tacgcgttcc aggtcaccca ggctctggtt ggtttggctg gcgacccagg
     9661 acatcgcacc aaagtcgaca tcgccactgc gggtacccat catcaagcct tccagcggcg
     9721 tcattcccat tgaagtatca acactctgac cgttgcgaac cgcgcagatt gacgcgccat
     9781 tgccaagatg cgccacaacc aggccggagt catcttccgc cagattcagc agcgaatgtg
     9841 cgcgctggga aacatagcgg tgcgacgtgc cgtggaaacc ataacggcgt acacctaact
     9901 cttcataata tttccacggc aggccgtata aataagcttc cggagccatc gtctggtgga
     9961 aactggtatc aaataccgcc acctgagtta cgcccggaaa taattgctgc gccgattcaa
    10021 taccacttaa attggcgtaa ttatgcaggg gtgccagtgg agaaacgcga cggatattat
    10081 caatgacttc atcggtaata atggcggact cggtaaaaat actgccgccg tgagcgatgc
    10141 ggtggccaat taaggccaca ctgtcattta aattccgttt ttccagttca aatgcaattg
    10201 ccttcaatgc accttcgtag ctgtggtgag ccagcggtgc tggctctccc ccatttacgg
    10261 ataagaatgc attttccgag ttaataccgt cggcaatacc tgacattaat acttcacagt
    10321 cgctggcatc gagtacggaa aacttaatcg aagacgaacc acagttaata accaaaacaa
    10381 ccggaaattc attcatctct tttctcatcc tgagttacgg attaaaacag tttgtatacg
    10441 atgttcagga tggtcagcag accaatcacg gtaacaaaca cgttatccag acgaccacgg
    10501 tatttcgcca gagacggcgc tttacggatg gcatacatcg gcaacaggca cagcagggat
    10561 gcgataatcg gtgcgcccat ggcttcaata aggtcgagga tgttcgggtt ggcgtaggca
    10621 acaacccagg tggagcccat gatgaagatc atgctgatag tgttcagttt acccagcgac
    10681 actttagttt tgtcgccttt ataaccgaac ttcagaatca gaccattcaa gccttccagc
    10741 gtccccagat agtgaccgaa gaaagatttg aagatagcca cgagtgcgat gatggaagcc
    10801 gcatattcca gtgtaatcgc gaacgttgtt ttggtaccgg tcatggacgc aaagtggtta
    10861 gccagataag aaagcactgg aatattctgc gctttggctt ccgccatgtt ggccggagac
    10921 agcgtaaaca ggcagctaaa ggcaaagaac atcaccactg caaccatcag catgctggca
    10981 cgagaaatga tttgggaaca tttacgttcg gtgaagtcgc gaccgaagtc tttctcatac
    11041 tcttcacgtt tagaaaccac gaaggaagag acgattggcg agaagttaaa ggagaaaacc
    11101 atgatggaaa tccccagcca gacagtgatc aggataccgt catgaccggt taacgacagc
    11161 gaaccgaggt caacctggtc gataactgca gagttccagt aagggatcag cgacagagaa
    11221 atcagcacca ggctggcgat aaacggccat accaggtagc tcatcacttt aaccatcaga
    11281 tccttaccaa accagatgac gaaagccatc agcagcaaca ggaacagcgc cacaaagccg
    11341 cgattcagcg gtgcaaatcc gagctggttt tcccagaacg tcataaaggt attggtaata
    11401 gtaacgccat aaatccacag cagtgggcaa atcgcgaaga agtacaggaa cgtgataacc
    11461 acgccgccag ttttaccaaa atgctcttcc accgtttccg taatgttgcc ggaagggtta
    11521 gagccagaaa gacacagacg cgccagcgcc cggtggcaat aaaatgcgat ggggtatgcc
    11581 aataccaaca tcagaagaat cgggatcagt ccgccaaaac ctgcgcggat agggaagaac
    11641 agcaccccgg cgccgattgc cgtaccaaac aagcctaacg tccatgtggt atctgattta
    11701 cgccaggacg attgttttgt ctggctggat acaatgctat ctgaagtact catatcctat
    11761 cctcaacgaa ttaattaagc gtcaacgaaa ccggtgattt gagagacgcg agaaagatcg
    11821 atattgccgc cggaaataat actgacggtt tttctgtttt gaatatattg gtctaattta
    11881 ccgcttaata atgcagcaca tgccagagcg cctgcgcctt cggtgacgac tttattgcgc
    11941 tgaattaagg caatcatact gtttctgatt tcgtcttcgc tgaccagcac gatgtcatcg
    12001 actaattcac gaacgatttc gtaagttaaa ttacccgggc gggagacatc acaaccatcc
    12061 gccagggtgc cggtagttcg gtgcgtggtt atttctccgg agtggaaaga agccgccatg
    12121 ccgtgaacgt tttctgactg tacgccaata acacgaatgg tcgggttaat agatttaatt
    12181 gccaccgcaa taccagcaat taaaccacca ccaccaattg gcacaatcac gttatcgaca
    12241 tcatagagat cttccataat ttccagacca atcgttccct ggccagcaat cactttcgga
    12301 tcatcgtaag gtgggataaa aatacgacct tccatttcga caatttcgct cactttagcg
    12361 atagtgtcgt tgaaattatc accatgcaga acgacttctg cggagtagtc gcacgttgcc
    12421 gctactttgg attttggcgc accttttggc atcaccactt taccgtcgat acccagcatc
    12481 gcgcaggaga gcgaaacccc ttgcgcatgg ttgcccgcag aacaggccac tacaccttta
    12541 cgtttttccg catcagtcag tgaacttaat ttattaaatg cgccacgaat tttaaatgaa
    12601 cccgtacgct gcatattttc aaacttcagg aatatttcac ctttgcaacg ttcactaaaa
    12661 tagtttgagc gaggcatgcc tgttttataa attcgcccag ccagtcgttg tttcgcttca
    12721 ataatgtcat caatagcaac cggcagatcg tatgtaatat gcattataaa acctcttcgc
    12781 ctgaattaaa tatgtaggta accgtaaccg acacctgcaa aacagacagg tggat
//
LOCUS       DAPHDW010000128        12652 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.130, whole genome shotgun sequence.
ACCESSION   DAPHDW010000128 DAPHDW010000000
VERSION     DAPHDW010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.130"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            42..1556
                     /gene="caiT"
                     /locus_tag="RH057_003545"
     CDS             42..1556
                     /gene="caiT"
                     /locus_tag="RH057_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HDV3129862.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLVGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            1587..2729
                     /gene="caiA"
                     /locus_tag="RH057_003546"
     CDS             1587..2729
                     /gene="caiA"
                     /locus_tag="RH057_003546"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HDV3129863.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            2858..4075
                     /gene="caiB"
                     /locus_tag="RH057_003547"
     CDS             2858..4075
                     /gene="caiB"
                     /locus_tag="RH057_003547"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HDV3129864.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLSTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            4149..5702
                     /gene="caiC"
                     /locus_tag="RH057_003548"
     CDS             4149..5702
                     /gene="caiC"
                     /locus_tag="RH057_003548"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HDV3129865.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGDKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESA
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYL
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQVQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPG
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYCDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            5811..6596
                     /gene="caiD"
                     /locus_tag="RH057_003549"
     CDS             5811..6596
                     /gene="caiD"
                     /locus_tag="RH057_003549"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HDV3129866.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPAIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIFRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            6602..7192
                     /gene="caiE"
                     /locus_tag="RH057_003550"
     CDS             6602..7192
                     /gene="caiE"
                     /locus_tag="RH057_003550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HDV3129867.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFHGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(7278..7673)
                     /gene="caiF"
                     /locus_tag="RH057_003551"
     CDS             complement(7278..7673)
                     /gene="caiF"
                     /locus_tag="RH057_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HDV3129868.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVAEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSRDKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(7934..11155)
                     /gene="carB"
                     /locus_tag="RH057_003552"
     CDS             complement(7934..11155)
                     /gene="carB"
                     /locus_tag="RH057_003552"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HDV3129869.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     ERLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(11173..12321)
                     /gene="carA"
                     /locus_tag="RH057_003553"
     CDS             complement(11173..12321)
                     /gene="carA"
                     /locus_tag="RH057_003553"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HDV3129870.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRTDKPAFSFQGHPEASPGPHDAAPLFDHFIELIEQYRKTAK"
BASE COUNT         2961 a         3294 c         3361 g         3036 t
ORIGIN      
        1 gaacgcaaaa taaatattcg ttttcacagt ggaattaact catgaagaat gaaaagagaa
       61 aaacgggaat agaaccgaag gttttctttc cgccgttaat aatcgtcggc atactttgtt
      121 ggcttacagt cagagatctg gatgcagcga atgtcgttat taatgctgta ttcagttacg
      181 tcaccaatgt atggggatgg gcatttgaat ggtatatggt ggtgatgctt ttcggttggt
      241 tctggctggt gtttggcccg tatgccaaaa agcgtttagg taacgaacca cctgaattta
      301 gtaccgccag ttggatcttt atgatgttcg cctcctgtac gtctgctgcc gtactgttct
      361 ggggatcgat tgagatctac tactacatct ccaccccgcc gtttggctta gaaccgaact
      421 cgacaggggc gaaagagttg gggctggctt acagcttgtt ccactgggga cctctgccgt
      481 gggccactta cagcttcctt tcagtcgcct tcgcttactt cttctttgtc cgcaaaatgg
      541 aagtgattcg ccccagctcg acactggtgc cgctggtagg tgaaaaacac gccaaagggt
      601 tgttcggcac tatcgtcgac aacttctatc tcgtcgcctt gatcttcgcg atgggtacca
      661 gtctgggcct tgccacgccg ctggtgaccg agtgtatgca atggttgttt ggcattccgc
      721 ataccctgca actggacgct atcatcatta cctgctggat tatcctcaac gccatttgcg
      781 tcgcttgcgg tctgcaaaaa ggggtacgta tcgccagtga cgtgcgtagt tacctgagct
      841 tcctgatgct gggttgggtg ttcattgtca gcggtgccag cttcatcatg aactacttca
      901 ccgattcggt ggggatgttg ctgatgtatc tgccgcgaat gttgttctat accgatccca
      961 tcgctaaagg cggcttcccg cagggctgga ccgtgttcta ctgggcatgg tgggtgattt
     1021 acgccatcca gatgagtatc ttcctcgccc gcatctcccg tggtcgtact gtgcgtgaac
     1081 tgtgcttcgg catggtgatg gggctgacag catccacctg gatcctgtgg accgtgctcg
     1141 gtagtaacac tctgctgttg atggataaaa acatcatcaa cattccaaat ctgatcgaac
     1201 agtacggtgt ggcgcgcgcc atcatcgaaa cctgggccgc tctgccactc agcaccgcca
     1261 ccatgtgggg cttcttcatc ctctgcttta ttgccaccgt tacgctggtt aacgcctgct
     1321 cttataccct ggcgatgtcc acttgccgcg aagtacgcga tggtgaagaa ccaccgctgc
     1381 tggtgcgtat cggttggtca attctggttg gcattatcgg tattgttctg ctggcgctcg
     1441 gcggcctgaa accgattcaa accgccatta tcgccggagg atgcccgctg ttcttcgtca
     1501 acattatggt gacgctctcc tttattaaag acgcgaaaca gaactggaaa gattaattaa
     1561 cccccaaaat atcaagaggt tgaaagatgg attttaattt aaatgatgag caggaactgt
     1621 ttgtcgccgg tatccgcgaa ctgatggcca gcgaaaactg ggaggcctat tttgccgagt
     1681 gcgaccgtga cagcgtctac ccggaacgtt ttgtcaaagc actggcggat atgggtatcg
     1741 acagtctgct gatccctgaa gagcacggtg gtctggacgc ggggtttgtt actctcgccg
     1801 ccgtgtggat ggagctggga cgtctggggg caccaaccta tgtgctgtac cagttgccgg
     1861 gcgggttcaa caccttcctg cgcgaaggca cacaagagca gatcgacaag attatggctt
     1921 tccgcggcac cggtaagcag atgtggaact cagcgattac cgaaccgggc gcgggctccg
     1981 acgtgggtag cctgaaaacg acttataccc gtagaaatgg taagatttat cttaatggta
     2041 gtaagtgttt tattaccagc agcgcctaca ccccgtacat cgtggtgatg gcgcgcgacg
     2101 gggcttctcc ggacaaacct gtctacaccg aatggtttgt tgatatgagc aaaccgggca
     2161 tcaaagtgac caaacttgaa aagctcggtc tgcgtatgga tagctgctgt gaaatcacct
     2221 ttgacgacgt ggaactggac gagaaagaca tgttcggtcg ggaaggtaac ggctttaacc
     2281 gcgtcaaaga agagttcgat catgaacgtt tcctggtagc cctcaccaac tacggtacgg
     2341 cgatgtgcgc ctttgaagat gcggcgcgct acgccaacca gcgcgtgcag tttggcgagg
     2401 ctattggtcg tttccagttg attcaggaaa aattcgccca catggcgatc aaattaaact
     2461 ccatgaaaaa catgctgtat gaagcagcgt ggaaagcaga caacggcacc atcacctctg
     2521 gcgatgcagc gatgtgcaaa tacttctgcg ccaatgcggc atttgaagtg gtggatagcg
     2581 caatgcaggt gctgggcggt gtcgggattg cgggcaacca ccgcatcagc cgcttctggc
     2641 gtgatctgcg tgtagaccgc gtctccgggg gatctgacga aatgcagatc ctgacgctgg
     2701 gtcgcgcggt gctgaagcaa taccgctaag tccgtgcgct gcctgatgcg acgctaatgc
     2761 gtcttatcag gcctacggga gcacgaatgt aggccggata aagcgtttac gccgcatccg
     2821 gcagtcatgc gctacataaa tttttcagga gctaataatg gatcatctac ccatgccgaa
     2881 attcgggccg ttggccggat tgcgcgttgt cttctccggt atcgaaatcg ccggaccgtt
     2941 tgccgggcaa atgttcgcag aatggggtgc ggaagttatc tggatcgaga acgtcgcctg
     3001 ggccgacacc attcgcgttc aaccgaacta cccgcagctc tcccgccgca atttgcacgc
     3061 gctgtcgtta aatattttca aagatgaagg ccgcgaagcg tttctgaaat taatggaaac
     3121 caccgatatc ttcatcgaag ccagtaaagg tccggccttt gcccgtcgtg gcattaccga
     3181 tgaagtactg tggcagcaca acccgaaact ggttatcgct cacctgtccg gttttggtca
     3241 gtacggcacc gaggagtaca ccaatcttcc ggcctataac accatcgccc aggccttcag
     3301 tggttacctg attcagaacg gtgatgttga ccagccaatg cctgccttcc cgtataccgc
     3361 cgattacttt tctggcctga ccgccaccac ggcggcgctg gcagcactgc ataaagtgcg
     3421 tgaaaccggt aaaggcgaaa gtatcgacat cgccatgtat gaagtgatgc tgcgtatggg
     3481 ccagtacttc atgatggatt acttcaacgg cggcgaaatg tgcccgcgca tgagcaaagg
     3541 taaagatccc tactacgccg gttgcggtct gtataaatgt gccgacggct acatcgtgat
     3601 ggaactggtg ggcattaccc aaattgaaga gtgctttaaa gatattggcc tcgcacatct
     3661 actcagtacg ccggaaatcc cggaaggcac tcagcttatc caccgtatcg aatgccctta
     3721 cggcccactg gtagaagaaa aactcgatgc ctggctggcg gcacatacca tcgcagaagt
     3781 taaagaacgc tttgccgaac tgaatatcgc ctgtgccaaa gtactgaccg taccggaact
     3841 ggaaagcaat ccacagtatg ttgcccgtga atcaatcact cagtggcaaa cgatggatgg
     3901 tcgcacctgc aaagggccga acatcatgcc gaaattcaaa aataaccccg gacaaatctg
     3961 gcgcggaatg ccctcacatg gcatggacac ggctgccatt ttgaaaaata tcggctacag
     4021 cgaaaacgac attcaggagt tggtcagcaa aggtctggcc aaagttgagg actaaacgcg
     4081 tggcgtcagt cggcttgcct gactagcgcc cgcccaataa caaacgaaca aaaatggata
     4141 gaggtgcaat ggatatcatt ggcggacaac atctacgtca aatgtgggac gatcttgcgg
     4201 acgtttacgg tcataaaacg gcgctgattt gtgaatccag cggcggagtc gttaaccggt
     4261 atagttatct tgagttaaat caggagatta accgcacggc aaacctgttt tatacgctgg
     4321 ggattcgcaa aggcgacaag gttgcactac atctcgacaa ctgcccggaa tttatctttt
     4381 gctggttcgg gctggcaaaa attggcgcga ttatggtgcc gattaacgcc cgcctgttac
     4441 gcgaggaaag cgcgtggatc ctgcaaaata gccaggcgtg cctgctggtg accagtgcgc
     4501 aattctatcc tatgtatcaa cagattcagc aggaagatgc cactcaattg cggcacattt
     4561 gcctgacaga tgtggcactt cccgctgatg atggcgtgag ttcgtttact caactgaaaa
     4621 atcaacaacc tgccaccttg tgctatgcac cgccgctatc gactgacgat acggcggaaa
     4681 ttctcttcac ctccggcacc acctcccgac cgaaaggtgt ggtgattacc cattacaacc
     4741 tgcgcttcgc tggatattac tccgcctggc agtgtgcact gcgtgacgat gacgtctacc
     4801 tgacggtaat gcctgcgttt catatcgatt gccagtgtac tgcggcgatg gcggcgtttt
     4861 ctgccggggc cacctttgtg ctggtcgaga aatacagcgc ccgcgccttc tggggacagg
     4921 tgcagaagta ccgcgccacc attaccgaat gtattccgat gatgatccgt acgttgatgg
     4981 tgcagccgcc ttcagcgaac gatcggcaac accgcctgcg ggaagtgatg ttttatctca
     5041 acttgtcgga gcaggaaaaa gatgcgtttt gtgaacgctt cggcgttcgc ttgctgacgt
     5101 cttatgggat gacggaaacc attgtgggca ttatcggcga tcgccctggc gataaacgac
     5161 gctggccgtc gattggtcgg gcggggtttt gctacgaagc ggagatccgc gacgatcaca
     5221 atcgcccgct cccggcaggt gagatcggtg aaatctgtat taaaggcgta ccagggaaaa
     5281 ccatcttcaa agagtatttt ctcaacccga aagccactgc gaaagtgctg gaagccgacg
     5341 gctggctaca taccggcgat accggatact gcgacgaaga gggctttttt tatttcgtcg
     5401 atcgccgctg caatatgatc aaacgcggtg gcgagaatgt ctcctgcgtg gagctggaaa
     5461 atatcatcgc cacccatcca aaaattcagg atatcgtggt tgtgggtatt aaagattcga
     5521 ttcgcgatga agccatcaaa gcatttgtgg tgctgaatga aggtgaaaca ttgagcgaag
     5581 aggaattttt ccgcttctgc gaacaaaata tggcgaaatt taaagtgccc tcttatctgg
     5641 agatcagaaa agatctgcca cgtaattgct cggggaaaat aattagaaag aatctgaaat
     5701 aaatgaaaca gcagggaact accctgccag ctaataatca cgctataaaa caatatgcat
     5761 tttttgcagg gatgttgtcg tccctgaaaa agcaaaaatg gagaaaagga atgagtgaat
     5821 cattacatct gacccgcaat ggatcaattc tggaaattac ccttgatcgt ccaaaagcga
     5881 atgctattga tgcaaaaacc agctttgaaa tgggcgaagt atttctaaat ttccgtgacg
     5941 atccgcaatt acgtgtcgcc attattaccg gtgccggaga gaagttcttt tccgcgggct
     6001 gggatttaaa agcggcagca gaaggcgaag caccggatgc tgactttggc ccgggtggat
     6061 ttgcgggatt aaccgaaatt ttcaatctcg acaaaccggt tatcgcagct gtgaacggct
     6121 atgcctttgg cggcggtttc gaactggcgc tggcggcaga ttttattgtt tgtgccgata
     6181 acgccagctt cgccctgccg gaagccaagc tgggcatcgt tcctgacagc ggcggcgtgc
     6241 tgcgtctacc gaagatcctg ccgcctgcca tcgtcaatga aatggtgatg accggcagac
     6301 gaatgggcgc agaagaggcg ctgcgttggg ggatagtcaa ccgcgtggtt agccaggcgg
     6361 aactgatgga taacgcccgc gaactggcgc agcagctggt taacagcgcc ccgctggcga
     6421 ttgcggcgct gaaagagatc ttccgcacca ccagcgaaat gccggtagaa gaagcgtatc
     6481 gctatattcg cagcggcgtg ttgaaacact atccgtcggt tctgcattcg gaggatgcca
     6541 ttgaagggcc gctggcgttt gccgagaagc gcgatccggt gtggaaagga cgttaacaac
     6601 cgtgagctat tacgcctttg aggggttaat tccggtggtt cacccgacgg cgtttgtcca
     6661 tcccagtgcc gtcttgattg gcgatgtgat tgtgggagcc ggtgtctaca tcggcccact
     6721 cgcctcactg cgtggtgact acgggcggtt gatcgtgcaa gcgggagcca atattcagga
     6781 tggctgcatt atgcatggct actgcgacac tgacaccatc gttggggaaa acggccatat
     6841 cgggcacgga gcgatcctgc atggttgtgt gattggtcgc gatgcattgg tcgggatgaa
     6901 cagcgtgatt atggatggcg cggtcattgg cgaagagagc attgttgccg ccatgagctt
     6961 tgtcaaagca ggctttcacg gcgagaaacg ccagttgttg atgggtacgc cagcccgcgc
     7021 tgtacgcagt gttagtgacg acgagttaca ctggaaacgg ttgaatacca aagagtatca
     7081 ggatcttgtc gggcgctgtc atgcatcgtt acatgaaaca cagccactga ggcaaatgga
     7141 ggaaaatcgc ccccgtttgc aggggacgac ggatgtgacg cctaaacggt gatcttgccg
     7201 gatgcgacct ggttgctgga taagatgcta caacatcgca tctggcaagc aggacgcccg
     7261 gtatcaacga gataaaatta acgacgcata ctctttgaca acatcatctg ccacttctgc
     7321 tcgcggttaa gttccgtgag cggaacggca ggaatacgcg gcgtctttct tacgccgact
     7381 aatttatccc ggctgttatt gcgcaggcgc gcgtaaattt gctcatcgat atcgaccact
     7441 tttaccagtc gctggcactg gcatccccgt ccttccagct tattagggat catcttaact
     7501 tcgcagctta tttctgcgac ttccgacaga atataagtca gggtattaac cgccttgctg
     7561 tgttcaatat cgaaatgaat agagatctct cttgctatca cccaccgatt ttcagccatc
     7621 atccattcgg cgattaacaa gtagagtggt ttttcaacat atccttcaca catacgaact
     7681 ccagtttcac aaattaaaag tctggctatg actcgttttg aagcaattaa acaaggccat
     7741 attaaaattg tggatatatc gatagtatgc aacttcaccg tcgataagtg tgagctaaat
     7801 cctgcttatg tcatcaaaaa tatcgattcc ccaaaagatt tacttaatgg gcaaaaaaaa
     7861 cggtcctcat cagagaaccg tattacttat tcgatcaaat tagcggatga aaaatatccg
     7921 ccatgacacg ctattatttg atctgcgcgt gcatttcctg caccgaaatt actttttcgg
     7981 tcgcgtcggc attcagcgcc atcgcggtag cgaaaccacc gttcagggtg gtgtcataat
     8041 gcactttata ttgcagcgca ctgcgacgga tcacgcggga gtcttcaatc gcgcgacggc
     8101 ctgaagtggt gttgatgatg taggtatatt cgccattctt gatgcggtcc tgaatgtgcg
     8161 gacggccttc atgcaccttg ttcaccagac gcggattaat gcccgcttcg cccagcacaa
     8221 tcgccgtgcc gtgggtcgca tccagctcga agccctgttt cagcagtttt gccgccaggt
     8281 ccaccacgcg ttctttatcg ccttcgcgca cggaaagcaa cgcacgaccg tgtttcttca
     8341 tggtggagtt gctgcccagc tgcgctttgg caaacgcttc agcgaaggtg cggcccacgc
     8401 ccatcacttc cccggtggag cgcatttctg gccctaacag cgggtcaaca cccgggaatt
     8461 tattgaacgg cagcaccact tctttcaccg agtagtacgg cgggataact tctttggtta
     8521 cgccctgctc agccagcgat ttgccagcca tcacacgcgc cgccactttt gccagcggta
     8581 cgccggtggc tttggagacg aacggaacgg tacgcgccgc acgcgggtta acttcaatca
     8641 ggtagacttc gttgtttttc accgcaaact gcacgttcat cagaccgcgc acctgcaatt
     8701 cgaaggccag tttctgcacc tgctggcgca tcacatcctg aatttcctga cttaaggtgt
     8761 aggctggcag agaacatgcg gagtcaccgg agtgcacgcc cgcctgctcg atgtgctcca
     8821 tgatgccgcc aataagcacc atttcgccgt cgcagatggc atccacgtca acttctaccg
     8881 cgtcatcaag gaagtgatcc agcaacactg gcgcatcgtt agacacgctg accgccgtct
     8941 ggaagtagcg acgcaggtca gcttcgtcat agacgatttc catcgcccga ccgccgagaa
     9001 cgtaagacgg acgtaccacc agcgggtagc caatctcttt cgccttctct accgccattt
     9061 caatagcggt aacggtggcg ttcgccggtt gtttcagttt cagacgctca accgcatgct
     9121 gaaagcgttc acggtcttct gcacggtcga tagcatccgg gctggtgccg ataaccggta
     9181 cgccagcagc ttccagcgcg cgcgccagtt tcagcggggt ctgaccgccg tactgaacga
     9241 taacgccttt cggcttctca atacgcacga tttctagcac atcttccaga gttaccggct
     9301 cgaagtagag gcgatcggaa gtgtcgtagt cggtggagac ggtttccggg ttacagttaa
     9361 ccataatggt ttcgtaaccg tcttcgcgca gcgccagcga ggcgtgtaca cagcagtagt
     9421 cgaattcgat accctgaccg atacggttcg ggccgccgcc gagaaccatg attttttcac
     9481 ggtcggtaga cggattcgct tcgcactctt cttcataagt ggagtacatg taagcggtgt
     9541 cggtggcgaa ctctgccgca caggtatcca cgcgcttata aaccgggtgc aggtcatact
     9601 ggtcgcgcag cttgcggatt tccgcttcgc gcacgcccgc cagttttgcc agacgcgcat
     9661 cggcaaagcc tttgcgtttc agctggcgca ggaattcagc gttcaggcca gtgatgccca
     9721 cttccgctac tttctcttcc agacgcacca gttcttcaat ctgtaccagg aaccaacggt
     9781 caatattggt caggttgaac acgccgtcca cagacaggcc cgcacggaag gcatcggcga
     9841 tgtaccagat acgctctgcg ccagcatctt tcagttcgcg acggattttg gttaacgctt
     9901 ccgggtcatc gaggctcact ttcgggtcga atccagtcgc accgacttcc aggccgcgca
     9961 gcgctttttg cagggattcc tgctgcgtgc gaccaatcgc catcacttcg ccaaccgatt
    10021 tcatctgagt ggtcagacgg tcgttagcac cagcgaattt ttcgaagttg aagcgaggaa
    10081 ttttagtaac cacatagtcg atggacggct cgaaggaggc cggagtacgt ccgccagtga
    10141 tgtcgttcat cagttcgtcg agggtgtaac ccaccgccag tttcgccgcc actttagcaa
    10201 tcgggaaacc ggtcgctttc gacgccagcg ccgaagaacg ggacacgcgc gggttcattt
    10261 cgataacaat caggcgaccg tttttcgggt tcaccgcaaa ctggacattg gaaccaccgg
    10321 tttcaacgcc gatttcacgc agcaccgcca tcgaggcgtt acgcatgatt tgatattctt
    10381 tgtcggtcag cgtttgggct ggcgcgacag tgatggagtc accggtatgg atgcccatcg
    10441 catcaaagtt ttcgatagag cagacgatga tgcagttgtc gtttttatca cgcaccactt
    10501 ccatctcgta ctctttccag ccaatcagcg actcatcaat cagcaactct ttggtcggag
    10561 agagatccag accgcgggcg caaatttctt caaactcttc gcggttataa gcgataccgc
    10621 cgccgctacc gcccatggta aaggatgggc gaataatgca cgggaagccc acgtcagcgg
    10681 caaccgccag cgcttcttcc atcgtgtgtg cgataccgga acgcgcggtt tccagaccaa
    10741 ttttcttcat cgctacgtcg aaacgacggc ggtcttctgc tttatcaatc gcatcggcgg
    10801 tcgcaccaat catagtcacg ccgaactctt ccaacacgcc ctgacgctcc agctccagcg
    10861 cgcagttcag cgccgtctga ccgcccatcg ttggcagcac cgcgtccggg cgctcttttt
    10921 caataatctt gcgtaccact tcccagtgaa tcggctcgat gtaggtcgca tcggccattt
    10981 ccgggtcggt catgatggtc gccgggttgg agttcaccag aatgacccgg taaccctctt
    11041 cgcgcagggc tttacatgct tgcgcgccag agtagtcaaa ctcacacgcc tgaccgataa
    11101 caatcgggcc cgcacccaga atcaggatac tttttatatc tgtacgtttt ggcatggctc
    11161 ttttactcct gattacttag cggttttacg gtactgctca attaactcga taaagtggtc
    11221 gaacaacggc gcggcgtcgt gtggaccagg gctggcttca gggtgcccct ggaagctgaa
    11281 cgccggttta tcggtgcgat gaatgccctg taacgtaccg tcgaacaggg atttatgcgt
    11341 gacacgcagg tttgctggta atgttgcttc gtccaccgca aaaccgtggt tctgggcggt
    11401 gatcatcacc acgtttttct caacatcttt aaccggatgg ttgccgccgt ggtgaccgaa
    11461 tttcattttg acagtcttcg caccgctcgc cagcgccagc agctgatgac cgaggcagat
    11521 gccgaagacc ggaatatcgg tttcgaggaa tttctggatg gcggtaatgg cgtaatcgca
    11581 cggggccggg tcgccaggac cgttggagag gaagatgccg tctggattca ttttcagcac
    11641 atcttccgca gaagtttgcg ccggaacgat ggtcaggcga cagcctctgt ccaccaacat
    11701 gcgcaggatg ttgcgcttgg caccaaaatc ataagccacg acgtggaacg gcagctcgtc
    11761 ttcttttttc gcttctggca ggccgccggt caacgtccag ctcccttgtg tccagctata
    11821 ggcttctgcg gtggtcactt cttttgccag atccatgcca ttcagacctg ggaacgcgcg
    11881 ggctttttct aacgccaacg ccgcatccgg gttatcgcct gcgataatgc agccattctg
    11941 tgcgcctttc tcgcgcagta aacgtgtcag cttacgggta tcgatatcgg caatcgccac
    12001 gatgttatgg cgcttcaggt aagaagagag gtcttcggta ttacggaagt tgctggcaat
    12061 cagcggcagg tcgcgaatca ccagaccttg tgcatgtacc tgagaagatt cttcatcggc
    12121 gtcattagtg ccgacattgc caatatgggg ataagtaaga gtaacgattt gacgagaata
    12181 ggaaggatca gtgaggattt cttgataacc ggtcattgaa gtattgaaaa cgacttcccc
    12241 aaccgccgaa cctgttgccc ctatggcccg accgtgaaac tgggttccgt cttccagaac
    12301 caatagcgct gacttaatca aaacaccctc cagagaatat tcactcactt tatttgcata
    12361 ttaattcaca atgatgacgt aaatcaatgc aaatctgctt accgacgaat ttctggcaaa
    12421 cggcggcatt ctggagatat gggcgctaaa agtcaactta atggcgggat tttttatatt
    12481 attttgtggc tcatgcatgt tttacaggca ttaagcggca aaaagatcat ataacctgtt
    12541 ctggaaaacg cttgcgcagc aagaagattg catgaaataa aagcgagtag ggaaaagtgg
    12601 accaaatggt caaaatttac attaaaacaa caaaaaccga agacaaaaca tg
//
LOCUS       DAPHDW010000129        12531 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.131, whole genome shotgun sequence.
ACCESSION   DAPHDW010000129 DAPHDW010000000
VERSION     DAPHDW010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12531
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.131"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(44..325)
                     /locus_tag="RH057_003554"
     CDS             complement(44..325)
                     /locus_tag="RH057_003554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HDV3129871.1"
                     /translation="MNKYWLSGIIFLAYGLASPAFSSETATLTINGRISPPTCSMAMV
                     NGQPQQHCGQLTYNVDTRHLFSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
     gene            complement(588..1697)
                     /gene="apbC"
                     /locus_tag="RH057_003555"
     CDS             complement(588..1697)
                     /gene="apbC"
                     /locus_tag="RH057_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HDV3129872.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMASKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAIVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            1829..3862
                     /gene="metG"
                     /locus_tag="RH057_003556"
     CDS             1829..3862
                     /gene="metG"
                     /locus_tag="RH057_003556"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HDV3129873.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPQLYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKAAAAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            4003..7797
                     /locus_tag="RH057_003557"
     CDS             4003..7797
                     /locus_tag="RH057_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR and DUF4132 domain-containing protein"
                     /protein_id="HDV3129874.1"
                     /translation="MRHFIYQDEKSHKFWAVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAAKAELKLIAEKVKKGYVEQAKDNSLQPSQTVTGSLKVADLSTIIQEQPSFVAET
                     RAPDKNTDAVLPWLAKDIAVVFPPEVVHTTLSHRRFPGVPVQQADKLTQLRRLACSVS
                     QRDNKTATFDFSACSLEWQNTVAQAISQIDGLKTTQLPSPVMAVLTALEMKCTRYKVR
                     EDVMDQIVQEGGLEYATDVIIHLQQIDIKWDYANNVIIILPSGIAPDYLEQYSRFELR
                     LRKHLSLAEESLWQKCAQKLIAAIPHIPEWRQPLIALLLPEKPEIAHEIAQRLLGQKK
                     LPSLEWLKIVATDEHILASLEEYHEPYAIFDDYYCGAIWSATVLQEQGVAALPRFAPY
                     AASDYCADVLRHINHPFALTLLIRVAGHTKRCHDRMTKACAAFPHAAMAALTELLGQK
                     EENSWRIMLMTMLISQPALAEQVIPWLSTPAVAVLKSCQQQLTQPSNHASADLLPAVV
                     VSPPWLSKKKKSPIPVLDLAPLGIEPICYLTEEISNQLLAKYIWYSKHITVSHEESTT
                     NLLARMGFQRRIAGTYIKAPEAVVEAWLNEDYSTLLSEFKVFHSPTGHYWQLGILTTL
                     PLEKAVKAWNALTLSPHTDTEYAMLHFGLKGLPGLVNSLARYPQEALPITNYFAASEL
                     APAVARAFNKLKTLRENARSWLLKYPEHALTGLLPAALGKAGEAQDNARAALRMLTEN
                     GHQPLLQEIARRYNQPEVTDAVNALLALDPLDNHPTKIPTLPAFYQPSLWTRPVLKAN
                     AQSLPDSALLHLGEMLRFPQEEALYPGLLQVKDVCSADSLAGFAWDLFTAWQTAGAPS
                     KESWAFTALGVLGNDDTARKLTPLIRAWPGESQHKRATVGLDILAAIGSDIALMQLNG
                     IAQKLKFKALQERAKEKIADIAESRELTVAELEDRLAPDLGLDDNGSLLLDFGPRQFT
                     VSFDETLKPFVRDVSGSRLKDLPKPNKSDDETRANDAVNRYKLLKKDARTIAAQQVAR
                     LESAMCLRRRWSLENFQLFLVEHPLVRHLTRRLIWGVYSAENQLLACFRVAEDNSYST
                     ADDDLFTLPEGDISIGTPHVLEISPTDAAAFGQLFADYELLPPFRQLDRNSYALTEAE
                     RNASELTRWAGRKCPSGRVMGLANKGWVRGEPQDGGWIGWMIKPLGRWSLIMEIDEGF
                     AVGMSPAELSAEQLLSKLWLWEGKAESYGWGSNSTQEAQFSVLDAITASELINDIEAL
                     FE"
     gene            7807..11439
                     /locus_tag="RH057_003558"
     CDS             7807..11439
                     /locus_tag="RH057_003558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
                     /protein_id="HDV3129875.1"
                     /translation="MDKELPWLADNAQLELKYKKGKTPLSHRNWPGEPVPVITESIIQ
                     TLGDELLQKAEKKKNIVWRYENFSLEWQSAITQAINLIGEHKPSIPARTMAALVCIAQ
                     NDSQQLLDEIVQQEGLEYATEVVIARQFITRCYESDPLLVTLQYQDEDYGYGYRSETY
                     NEFDLRLRKHLSLAEESSWQRCADKLIAALPGIPKIRRPFIALLLPEKPEIANELASL
                     ESSRSSLHSKEWLKVIATDNTAVKKLERCWGLDVFSDREASYMSHENHFGYAACAALL
                     REQGLAAVPRLAIYAHKEDCGSLLVQINHPQVIRTLLLVADKNKPSLQRVAKYSKNFP
                     HATLAALAELLALKEPPARPGYPIIEDKKLPAQQKARDEYWRTLLQTLIASQPQLAER
                     VMPWLSTQARAVLNSYLSAPPKPVIDSTDNSSLPEMLVSPPWRSKKKMTAPRLDLAPL
                     ELTPQIYWQPGEQERLAATESARYFSTESLAERMEQKSGRVVLQELGFGDDVWLFLNY
                     ILPGKLDAARNSLIVQWHYYQGRVEEILNGWNSPQAQLAEQALRSGHIEALINIWEND
                     NFSRYRPEKSVWNLYLLAQLPREMALTFWLRIIEKKHLFAGEDYFLSILGLDALPGLL
                     LAFSHRPKETFPLILNFGATELALPVARVWRRFAAQRDLARQWILQWPEHTASALIPL
                     VFTKPSDNSEAALLALRLLYEQGHGELLQTVANRWQRTDVWSALEQLLKQGPMDIYPA
                     RIPKAPDFWHPAMWSRPRLITNNQPVTGDALEIIGEMLRFTQGGRFYSGLEQLKTFCQ
                     PQTLAAFAWDLFTAWQQAGAPAKDNWAFLALSLFGDESTARDLTTQILAWPQEGKSAR
                     AVSGLNILTLMNNDMALIQLHHISQRAKSRPLRDNAAEFLQVVAENRGLSQEELADRL
                     VPTLGLDDPQALSFDFGPRQFTVRFDENLNPVIFDQQNVRQKSVPRLRADDDQLKAPE
                     ALARLKGLKKDATQVSKNLLPRLEAALRTTRRWSLADFHTLFVNHPFTRLVTQRFIWG
                     VYPANEPRRLLNAFRVAAEGEFCNAQDEPIDLPADALIGIAHPLEMTAEMRSEFAQLF
                     ADYEIIPPLRQLTRRTVLLTPDESASNSLNRWEGKSATVGQLMGMRYKGWESGYEDAF
                     VYDLGEYRLVLKFSPGFNHYNVDSKALMSFRSLRVYSDNKSVTFAELDVFDLSEALSA
                     PDVIFH"
     gene            11500..11820
                     /locus_tag="RH057_003559"
     CDS             11500..11820
                     /locus_tag="RH057_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129876.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDADPLINDALIYIRDECIDERKLRGESPETVIAIFNRELQNIFNQ
                     LIEY"
BASE COUNT         3290 a         3180 c         3049 g         3012 t
ORIGIN      
        1 atgatttcgg ctttctaaat gcaattaaaa tcaggttgcc aaattaatcg tagcgattta
       61 acacgattcg acgtttgcta tcgctacctg caacaaccac ttctgtcgtt acacctttga
      121 cgggcgaaga aaacagatga cgggtgtcaa cattatacgt taactgacca caatgctgtt
      181 gaggttgacc attaaccata gccatactgc atgttggggg actgatcctg ccattaatcg
      241 ttaatgtggc agtctctgaa gagaatgcgg gagaagccaa tccataggca agaaaaataa
      301 ttcctgacag ccaatactta ttcattgaac gttatccctg tagtaaaggt tatgcctgga
      361 tagaatgagt gcataacaaa ctatagctgt acatccacta cacagccacg aaggatgata
      421 atgaagcatt gcctgtatga tcaatcgact ttgtagagtt tcggacgaag gtccgcagaa
      481 tattcgcagt attaaataag tgttcattaa tgcggcaaaa actaatacac cgcatcaatg
      541 taacatctct gcggtgtatg aagaataacc agaatggtta ataggcgtta gaccgcgcgg
      601 aaggaaatct cgcctggaat gacttcaccc tgccagtaga gctgagctgc aacgcggtca
      661 gccagctggc ggtagatagc ggtaaattcg ctctccggac ggctgataac cgttggcgtg
      721 cctttatcga gatcctcacg taaggagatg tgtaacggca tctgccccag cagctgtgtg
      781 tggtattttt cagccagttt ctctgcacca ccggtaccga aaatcggttc atggtgacca
      841 cagttactgc aaatatgcac actcatgttc tcgacaatac caagcaccgg aacctcgact
      901 ttctcgaaca tcacaatgcc ttttttcgca tcgatcaacg cgatgtcctg cggggtggtg
      961 accacgattg caccagttac tggaatgttt tgcgccagcg tcaattgaat gtcaccggtg
     1021 cctggcggca tatcaagcac cagatagtcg agatccggcc acaaggtttc ctgcaacatc
     1081 tgcattaacg ccttgctcgc catcggtcca cgccacacca tcgcattgtc gtcagtgacc
     1141 agataaccaa ttgaattggt tgccaggcca tgagacataa tcggtgccat atgggtaccg
     1201 tccggcgaag ttggacgctg attttctgcg cccagcatgg tgggaattga tgggccgtag
     1261 atatcggcgt ccagaatgcc gactttcgcc ccttccgccg ccagtgccag cgccaggttt
     1321 accgccgtgg aggatttacc cacgccgcct ttaccggaac tgaccgcaat gatgttcttc
     1381 acaccattaa tacccggctg attcttcacg cgttttaagg tggcgatatt gtgcgacagt
     1441 ttccagtcga tagctttcgc cccggtgata cgcagcagtt cggcgctaca ttgctctttt
     1501 aactcttcaa acgcgctatg ccagacgaat ggcataacca gttccacatg caacgtgtcg
     1561 tccatccagg cgacatggtg caacgctttc agcgtggtga ggttatgctt cagggttggg
     1621 tgctgaaaat tggccagtgt cccggcgacc attgcgcgca aggcttctgg tgatttggcc
     1681 tgggattgtt cgttcatccc gactcctttt ttgtatagat aaaccatcag ctgatagttt
     1741 acctgaagaa tatagagaag tacttactta acattttccc atttggtact atctaacccc
     1801 ttttcactat taagaagtaa tgcctactat gactcaagtc gcgaagaaaa ttctggtgac
     1861 gtgcgcactg ccgtacgcta acggctcaat ccacctcggc catatgctgg agcacatcca
     1921 ggctgatgtc tgggtccgtt accagcgaat gcgcggccac gaggtcaact tcatctgcgc
     1981 cgacgatgcc cacggtacac cgatcatgct gaaagctcag cagctcggta tcaccccgga
     2041 gcagatgatt ggcgaaatga gtcaggagca tcagactgat ttcgcaggct ttaacatcag
     2101 ctatgacaac tatcactcga cgcacagcga agagaaccgc cagttgtcag aacttatcta
     2161 ctctcgcctg aaagaaaacg gttttattaa aaaccgcacc atctctcagc tgtacgatcc
     2221 ggaaaaaggc atgttcctgc cggaccgttt tgtgaaaggc acctgcccga aatgtaaatc
     2281 cccggatcaa tacggcgata actgcgaagt ctgcggcgcg acctacagcc cgactgaatt
     2341 gatcgagccg aaatcggtgg tttctggcgc tacgccggta atgcgtgatt ctgaacactt
     2401 cttctttgat ctgccctctt tcagcgaaat gttgcaggca tggacccgca gcggtgcgtt
     2461 gcaggagcag gtggcaaata aaatgcagga gtggtttgaa tccggcctgc aacagtggga
     2521 tatctcccgc gacgcgccat actttggttt tgaaattccg aacgcgccgg gcaaatattt
     2581 ctacgtctgg ctggacgcac cgattggcta catgggttct ttcaagaatc tgtgcgacaa
     2641 gcgcggcgac agcgtaagct tcgatgaata ctggaagaaa gactccaccg ccgagctgta
     2701 ccacttcatc ggtaaagata ttgtttactt ccacagcctg ttctggcctg ccatgctgga
     2761 aggcagcaac ttccgcaagc cgaccaacct gtttgttcat ggctatgtga cggtgaacgg
     2821 cgcaaagatg tccaagtctc gcggcacctt tattaaagcc agcacctggc tgaatcattt
     2881 tgacgctgac agcctgcgtt actactacac tgcgaaactc tcttcgcgca ttgatgatat
     2941 cgatctcaac ctggaggatt tcgttcagcg tgtgaatgcc gatatcgtga acaaagtggt
     3001 taacctggcc tctcgtaatg cgggctttat caacaagcgt tttgacggcg tgctggcaag
     3061 cgaattggct gacccgcagt tgtacaaaac cttcactgat gccgctgaag tgattggtga
     3121 agcgtgggaa agccgcgaat ttggtaaagc agtgcgcgaa atcatggcgc tggctgatct
     3181 ggctaaccgc tatgtcgacg agcaggctcc gtgggtggtg gcaaaacagg aaggccgcga
     3241 tgccgacctg caggcaattt gctcaatggg cataaacctg ttccgcgtgc tgatgactta
     3301 cctgaagccg gtactgccga aactgaccga gcgcgcagaa gcattcctca atacagaact
     3361 gacctgggat ggtatccagc aaccgctgct gggccacaaa gtgaatccgt tcaaagcgct
     3421 gtataaccgc atcgatatga agcaggttga agcactggtg gaagcgtcta aagaagaagt
     3481 gaaagcagct gccgcgccag ttactggccc actggcagat gatccgattc aggaaaccat
     3541 cacctttgac gacttcgcaa aagttgacct gcgcgtggcg ctgatcgaaa acgcagagtt
     3601 tgttgaaggt tctgacaaac tgctgcgcct gacactggat ctcggcggtg aaaaacgcaa
     3661 tgtcttctcc ggtattcgtt ctgcctaccc ggatccgcag gcactgatcg ggcgtcacac
     3721 cattatggtg gccaacctgg caccgcgtaa aatgcgcttt ggtatctccg aaggcatggt
     3781 aatggctgct ggtcctggcg ggaaagatat cttcctgtta agcccggatg ctggtgctaa
     3841 accaggccat caggtgaaat aatccccctt taaggcgctg catcgacagc gccttttctt
     3901 tataaattcc taaagttgtt ttcttgcgat tttgtctctc tctaacccgc ataaatactg
     3961 gtagcatctg cattcaactg gataaaatta cagggatgca gaatgagaca ctttatctat
     4021 caggacgaaa aatcacataa attctgggca gttgagcaac agggaaacga gttgcatatc
     4081 agttggggaa aagttggcac caaagggcaa agccagataa aaagtttttc agatgctgcg
     4141 gcagcggcaa aagcggagct taagctgatt gcggagaagg tgaagaaggg gtatgtggag
     4201 caagcgaagg ataattcttt gcaaccttcc caaacggtaa cgggctctct caaggtagcg
     4261 gacttatcca ccattattca ggaacaaccc tctttcgtag cagaaacccg tgcgcctgac
     4321 aaaaatacag atgctgtttt accgtggctg gcgaaagata ttgctgtcgt ttttccgccc
     4381 gaagttgtac acaccacgtt aagtcatcgc cgctttcccg gagttcctgt tcagcaagca
     4441 gacaaattga cccaattacg tcgcttagcc tgtagtgtgt cgcaacggga taataaaaca
     4501 gccacatttg acttcagcgc ctgttcttta gaatggcaaa acaccgtcgc ccaggcgatc
     4561 agtcagatcg acggcctgaa aacaacacag ctaccatcac cagtaatggc tgtactcacg
     4621 gcacttgaaa tgaaatgcac aagatataaa gtgcgtgagg atgttatgga tcagatcgtc
     4681 caggaaggcg gtctggaata tgctactgat gtaataatac accttcaaca gattgatatt
     4741 aaatgggatt atgcgaataa tgtcattatt attctgccgt ctggcattgc acctgactac
     4801 ttggagcaat attccagatt tgaattacgc ctacgtaaac atttatcact ggcggaagag
     4861 tctctctggc aaaaatgtgc acaaaaactt attgccgcaa ttccacatat tccagaatgg
     4921 cggcaaccat taattgcttt gttattaccc gaaaaaccag aaattgcaca tgaaattgcc
     4981 cagcgtctac tggggcaaaa aaaattaccc tcgcttgagt ggttaaaaat agtggcgact
     5041 gatgagcaca ttcttgcctc attagaagaa tatcacgagc catatgccat ttttgatgat
     5101 tactattgtg gtgcgatatg gtcagccacc gtattacagg agcaaggtgt tgcagccctg
     5161 ccccgatttg ctccctatgc cgcaagtgac tactgcgccg atgtgttgcg tcatatcaat
     5221 catccgttcg cattaacact gcttatacgt gtagccgggc atactaaacg ctgtcacgat
     5281 cggatgacga aagcctgcgc tgcgttccca catgcagcaa tggcggcact gacggaactt
     5341 cttgggcaaa aagaagagaa cagttggcgc attatgctaa tgacaatgct tatctcacaa
     5401 ccagcactgg cagaacaggt cattccctgg ctctcgacac ccgcagttgc cgtactgaaa
     5461 tcatgccagc aacaactgac acagccctca aaccatgcca gcgccgatct actgccagcc
     5521 gtagtagtct cccctccctg gctttcgaaa aagaaaaaat cgccgattcc ggtgctggat
     5581 ttagcgccat taggcattga gccaatctgt tatctgacag aagaaatcag taatcaactt
     5641 ttggcgaaat atatctggta ttcaaaacac atcacggtta gccatgaaga aagtactacc
     5701 aacctgttgg caaggatggg ttttcaacga cggatcgctg gtacatatat taaagctccc
     5761 gaagcggtag ttgaggcatg gctaaatgaa gattattcaa ccttactaag tgaatttaag
     5821 gtgtttcatt cacctaccgg gcattattgg cagttgggga ttttgacaac attgccgctg
     5881 gagaaagcag taaaagcatg gaatgccctt accctatctc cacataccga taccgaatac
     5941 gccatgttac attttggact caaagggtta cctgggttag taaactcact tgcacgctat
     6001 ccacaagaag ccttgcccat cacgaattac ttcgcagcga gtgagctggc tcctgccgtc
     6061 gcccgtgcct tcaacaaact gaaaacgcta cgcgaaaacg cccgtagctg gctgttgaaa
     6121 tacccggaac atgcccttac cggcctgctg cctgcggcgc tcggcaaagc cggtgaagca
     6181 caggataacg cccgcgctgc cttgcgtatg cttaccgaaa acggtcatca gccattactg
     6241 caagaaatcg cccgacgtta taaccagccg gaagtaaccg atgcggtgaa cgctctgctt
     6301 gcgctcgatc ccttagataa tcacccgaca aaaatcccca ctcttccggc cttttatcag
     6361 ccatcgctct ggacgcgccc ggtattaaaa gcaaatgccc aatcactgcc agatagcgcc
     6421 ctcctccacc tcggtgaaat gctccgcttc cctcaggaag aggctctgta tccgggatta
     6481 ttgcaggtga aagacgtctg ttccgccgac tcactggcgg gatttgcctg ggatctgttt
     6541 accgcctggc agaccgctgg cgcgccgtcg aaagagagtt gggcgttcac tgcgttaggc
     6601 gttctcggta acgatgacac cgcccgcaaa ctgacgccat taatacgcgc ctggcctggt
     6661 gaatcccagc ataaacgcgc caccgttggg ttggatattc tcgctgctat cggtagtgat
     6721 atcgccctta tgcagcttaa cggcatcgcc cagaaactga aattcaaagc attacaggag
     6781 cgggcaaaag aaaaaattgc cgacattgcc gagagccgcg aactcacggt ggcggagctt
     6841 gaagatcggt tagcaccgga tctcggtctg gatgataacg gttcgctgct gctggatttt
     6901 ggcccacggc agttcaccgt cagctttgat gaaaccttaa aaccgtttgt gcgtgatgtt
     6961 tccggcagcc gcctgaaaga cctgcccaaa ccgaacaaaa gcgatgatga aacgcgggcg
     7021 aacgatgcgg ttaaccgcta caaattgctg aaaaaagatg cgcgtaccat cgccgcccag
     7081 caggtagcaa ggctggaatc cgccatgtgc ctgcgccgcc gctggtcgct ggaaaacttc
     7141 cagctcttcc tggttgagca tccgctggtt cgtcacttaa cccgccgtct gatttggggc
     7201 gtttatagcg ccgaaaacca gctactggct tgctttcgcg tagcagaaga taacagctac
     7261 agcaccgctg acgatgatct tttcaccctg ccggaaggcg atatctctat cggcactcct
     7321 cacgttctgg aaatatcacc aacggatgct gccgcctttg gtcagctttt tgccgactac
     7381 gaactgttac caccgttccg ccagctcgac cgtaacagct acgccctgac agaagccgag
     7441 cgcaatgcca gtgaactgac ccgctgggca ggcagaaaat gcccgagcgg tcgggtcatg
     7501 ggactggcga ataaaggttg ggtgcgtggt gaaccgcagg atggtggctg gatcggctgg
     7561 atgatcaaac ctctgggccg ctggtcgtta atcatggaaa tcgatgaagg ctttgcagtt
     7621 ggtatgtcgc cagccgaact tagcgccgag caactcttaa gcaagctgtg gctatgggaa
     7681 ggcaaagcag aaagctatgg ctgggggagt aattcaacac aggaagcgca gttctccgta
     7741 ctcgatgcca tcaccgccag cgagctgatt aacgatattg aagccctgtt tgaataagga
     7801 aagagcatgg acaaggaatt accgtggctg gcggataacg cccaactgga actgaaatat
     7861 aaaaaaggca aaacaccact cagccatcgc aattggccgg gcgaaccagt gcctgttatc
     7921 actgaaagca tcattcagac actgggtgat gaattgctac aaaaagctga gaagaaaaaa
     7981 aacattgtct ggcgttatga gaatttttca ctggagtggc agtccgccat tacgcaggcc
     8041 atcaacttga tcggcgaaca caaaccctca atcccggccc ggacaatggc ggcgctagtc
     8101 tgtatcgcgc aaaatgacag ccaacagttg ctcgacgaaa tcgtccaaca agagggactg
     8161 gaatatgcga ctgaggtggt gattgcacgc cagtttatta cacgatgtta tgagagtgat
     8221 cctctgctag tgacattgca gtatcaggac gaggattatg gctatggtta tcgctcagaa
     8281 acctataacg aattcgatct ccgactgcgt aagcatctct ctctggcaga ggaaagcagc
     8341 tggcagcgtt gcgccgacaa actcattgcc gcactgccag gaatacccaa aattcgccgt
     8401 ccttttattg cactactcct cccggaaaaa cctgaaatag ccaatgaact cgccagtctt
     8461 gaaagctccc ggtcaagtct ccattcaaag gagtggttaa aggtcattgc tactgataat
     8521 acggcggtaa aaaaactcga acgatgctgg ggcctggacg tatttagcga tcgagaagcc
     8581 agctacatgt cgcatgaaaa ccacttcggc tacgcggcct gcgccgccct tttgcgcgaa
     8641 caaggactgg cagccgttcc gcgcctcgcg atatatgccc ataaagaaga ttgcggcagt
     8701 ctgctggtac aaattaacca tccgcaagtc atccgcacct tgctgttagt tgctgataaa
     8761 aacaaaccca gcctgcaacg tgtggctaaa tacagtaaaa acttccccca tgcgacactc
     8821 gccgcactgg cagaactgct ggcgttaaaa gaaccaccag cccgcccagg ttatccaatc
     8881 atcgaagaca aaaagctgcc tgcacagcaa aaagcacgcg atgaatactg gcgtacgctg
     8941 ttacaaacgc tgatagcatc gcagccacaa ctagcagaaa gggtgatgcc gtggttaagt
     9001 actcaagcca gggcagtgct gaatagttat ttatcggcac cgccaaaacc ggttattgat
     9061 agcaccgata acagcagtct gcctgaaatg ctcgtttcac caccgtggcg tagtaagaaa
     9121 aaaatgacag ctccacgtct tgatttggca ccgctcgaat taactccgca aatttactgg
     9181 caaccaggcg aacaagagag gcttgccgcc actgagtctg cccgttattt cagcacggaa
     9241 tctcttgcgg aacgcatgga acaaaaaagt ggacgagttg tattacagga actgggtttt
     9301 ggggatgatg tatggctgtt tctgaattat atactccccg gaaaactgga tgctgcacgc
     9361 aattcactca ttgttcagtg gcattactac caggggcggg ttgaagagat cctgaatggc
     9421 tggaactccc cgcaagcaca attagcagaa caggcgctcc gcagcggtca catagaagcg
     9481 ttaattaaca tatgggaaaa tgacaacttc tcacgttatc gtccagaaaa gagtgtctgg
     9541 aacctgtatt tattggcaca gttgccgcgt gagatggctt tgaccttctg gctgcgtatc
     9601 attgagaaaa agcatctgtt cgcgggtgag gactattttc tcagtatcct cggattggat
     9661 gcgctaccag gtctgctgtt ggctttttca catcgtccaa aagaaacatt tccgttaatt
     9721 ttaaattttg gcgcaacaga actggcgctg cctgttgccc gcgtctggcg acgtttcgcg
     9781 gcgcagcgtg atctggctcg ccagtggatt ttacaatggc cggaacatac ggctagtgca
     9841 cttatccctc ttgtctttac caaacccagc gataatagcg aagccgcatt acttgccctg
     9901 cgtttactgt acgaacaggg acatggcgaa ttgctacaaa ccgtggcaaa ccgctggcag
     9961 cgtacagatg tatggtctgc cctggagcag ttgcttaaac agggtccaat ggacatttac
    10021 ccggcacgca ttccaaaagc ccctgatttc tggcatccgg caatgtggtc caggcctcga
    10081 cttatcacta ataatcagcc tgttaccggt gacgctctgg aaattatcgg cgaaatgctg
    10141 cgctttaccc aggggggacg tttttatagc gggctggaac aactgaaaac gttctgccag
    10201 ccacaaacgc tggcagcttt tgcctgggat ctcttcactg cgtggcaaca agctggtgcc
    10261 cccgcaaaag acaactgggc atttctggcg ttaagtctct ttggtgacga aagcacggca
    10321 cgggatctga cgacacagat cctcgcctgg ccacaagaag gcaaatctgc ccgtgctgtc
    10381 agcggcctga acatccttac cctgatgaat aatgatatgg cgctgataca gctgcatcat
    10441 atatcgcaac gggctaaatc ccgcccctta cgtgataacg cggcggaatt tcttcaggtg
    10501 gtcgcagaaa atcgcgggct aagccaggaa gagctagcgg acagattagt cccaaccctg
    10561 ggccttgatg atccgcaggc gttgagtttt gattttggtc cccggcagtt taccgttcgc
    10621 ttcgatgaaa acctcaaccc ggttatcttt gatcagcaaa acgttcgcca gaaaagcgtt
    10681 ccccggttgc gcgccgatga cgatcaactg aaagcgcccg aggcactggc ccgactaaaa
    10741 gggctaaaaa aagatgctac tcaggtgagc aaaaacctgc tcccgcgtct tgaagctgcc
    10801 ctacgtacca cccgacgctg gtcgctggca gattttcata ctctgtttgt taatcatccc
    10861 tttacccgcc tggttaccca gcgattcata tggggcgtgt atccggcaaa tgaaccgcgt
    10921 cgtttactca acgcctttcg tgtggccgca gagggggagt tctgcaatgc gcaggatgag
    10981 ccaattgacc tgcctgcgga tgctctgatt ggcattgccc acccgttaga aatgacagca
    11041 gaaatgcgca gtgaatttgc ccagcttttt gccgattacg aaattatacc gccacttcgc
    11101 cagttgacac ggcgcacggt gctgctcacg cctgacgagt cagccagtaa tagtttgaat
    11161 cgctgggaag gtaaatctgc taccgttggg caacttatgg gaatgcgata caaaggctgg
    11221 gagtcaggct atgaggacgc atttgtctat gacctgggtg agtaccggct ggtccttaag
    11281 ttttcacccg gttttaacca ctacaatgtt gatagcaaag cgctaatgag cttccgttct
    11341 cttcgagtgt acagtgacaa taaatccgtc acttttgccg aacttgatgt gtttgatttg
    11401 agtgaggcgt taagcgcacc tgacgtcatt ttccattaaa acacagcggg cagtgatgca
    11461 actgcccgtt atcaacacga cttaccagcg gggaaagcga tgatcgtgca aaaagagctg
    11521 gttgctattt acgattatga ggtccctgta cctgaagatc cgttttcctt cagacttgag
    11581 atccataaat gctctgaatt atttacaggt tccgtctatc gactggagcg attccggcta
    11641 cgtccaacat ttcatcaacg tgatcgagaa gatgctgacc cgctaataaa tgatgcgttg
    11701 atttatataa gagatgagtg tattgatgag cggaaattac gaggtgaatc acctgaaact
    11761 gtaatagcaa tttttaatcg tgaactacag aatatattca accaactaat agaatattag
    11821 tttattaaag ggggaagcgg ttttccccct tagttgcgtt tcaatttgcc cattacttca
    11881 acaaatcaat caaccgtaac agagacacta atagctccag tacggcaatg agcacggtta
    11941 acttttgtgt cagcgtgtcc atccgctctc cttattaagg agcgcaacac cagcacctta
    12001 cccttacact gcctgtcgat actggtttac gtttgttgag tcgccatgtt gcactccgga
    12061 tcagagcact acctgcggca ccacccgtcc ggtagccagg acttgaaatg ggttcgatcc
    12121 ggcgcaactt cccaacagtc aggattataa atctcattat cttaattgca acactgtcca
    12181 gaggtggtta taattcgttc gttgagatgc catgttgcac tatcagtaac ttaacatcaa
    12241 agtagtaaat aaaagaacct gcctcaccag caggtttttt tattttctgc gatctacttt
    12301 ccagatattt tttgccaata gcccaccata aatattcaca tcccggtcaa atggtgcgtt
    12361 gaatataaaa tcgatgaatt tataaataaa aatgagttat atgatgaatt aataaaaata
    12421 gtaatcattt tattaaaggg ggaagcggtt ttccccctta gatgcgtttc aatttgcccg
    12481 ttacttcaac aaatcaatca accgtaacag agccactaat agctccagta c
//
LOCUS       DAPHDW010000130        12328 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.132, whole genome shotgun sequence.
ACCESSION   DAPHDW010000130 DAPHDW010000000
VERSION     DAPHDW010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12328
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.132"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..310)
                     /gene="cdiI"
                     /locus_tag="RH057_003560"
     CDS             complement(<1..310)
                     /gene="cdiI"
                     /locus_tag="RH057_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-immunity protein system imunity protein
                     CdiI"
                     /protein_id="HDV3129877.1"
                     /translation="MITLRKLIGNINMTKEPEQQSPLELWFERIIDVPLEKLTVEDLC
                     RAIRQNLCIDQLMPRVLEVLTKEPLAGEYYDGELIAALSTIKGEDLKDQKSTFTQIRQ
                     L"
     gene            complement(307..10098)
                     /gene="cdiA"
                     /locus_tag="RH057_003561"
     CDS             complement(307..10098)
                     /gene="cdiA"
                     /locus_tag="RH057_003561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contact-dependent inhibition effector tRNA
                     nuclease"
                     /protein_id="HDV3129878.1"
                     /translation="MNQPPVHFTYRLLSYLVSAIIAGQPLLPAVGAVITPQNGAGMDK
                     AANGVPVVNIATPNGAGISHNRFTDYNVGKEGLILNNATGKLNPTQLGGLIQNNPNLK
                     AGGEAKGIINEVTGGKRSLLQGYTEVAGKAANVMVANPYGITCDGCGFINTPHATLTT
                     GRPVMNADGSLQALEVTEGSITINGAGLDGTRSDAVSIIARATEVNAALHAKDLTVTA
                     GANRVTADGRVSALKGEGDVPKVAVDTGALGGMYARRIHLTSTESGVGVNLGNLYARD
                     GDITLDASGRLTVNNSLATGAVTAKGQGVTLTGDHKAGGNLSVSSRSDIVLSNGTLNS
                     DKDLSLTAGGRITQQNEKLTAGRDVTLAAKNITQDTASQINAARDIVTDASDTLTTQG
                     QITAGQNLTASATTLTQDGILLAKGHAGLDAGTLNNSGAVQGASLTLGSTTLSNSGSL
                     LSGGPLTVNTRDFNQSGRTGAKGKVDITASGKLTSTGSLVSDDVLVLKAQDVTQNGVL
                     SGGKGLTVSAQTLSSGKKSVTHSDAAMTLNVTTVALDGETSAGDTLRVQADKLSTAAG
                     AQLQSGKNLSINARDARLAGTQAAQQTMVVNASEKLTHSGKSSAPSLSLSAPELTSSG
                     VLVGSALNTQSQTLTNSGLLQGEASLTVNTQRLDNQQNGTLYSAADLTLDIPDIRNSG
                     LITGDNGLTLNTASLSNPGKIIADTLNVRATTLDGDGLLQGAGALALAGDTLSQGRNG
                     RWLTAGDLSLRGKTLNTAGTTQGQNLTVQADNWANSGSVLATGNLTASASGQLTSTGD
                     IMSQGDTTLKAATTDNRGSLLSAGTLSLDGNSLDNSGTVQGNHVTIRQNGVTNSGTLT
                     GIAALTLAARMASPQPALMNNGGSLLTSGDLTITAGSITSSGHWQGKRVLITSDSLAN
                     SGAIQAADSLTARLTGELVSTAGSKVTSNGEMALSALNLSNSGQWIAKNLTLKANSLT
                     SAGDITGVDALTLMVNQTLNNHASGKLLSAGVLTLKADSVTNDGQLQGNATTITAGQL
                     TNGGHLQGETLTLAVSGGVNNRSGGVLLSRNALNVSTTTLSNQGTIQGGGGASLNATD
                     RLQNDGKILSGGNLTLTAQALANTGSGLVQAVTLLLDVVNAVNGGRVLATGSTDVKGT
                     SLNNSGTLQGADLLVNYHTFSNSGTLLGTSGLGVKGSSLLQNGTGRLYSAGNLLLDAQ
                     DFSGQGQVVATGDVTLKLIAALTNHGTLAAGKTLSVTSQNAITNGGVMQGDAMVLGAG
                     EAFTNNGTLTAGKGNSVFSAQRLFLNAPGSLQAGGDVSLNSRSDITISGFTGTAGSLT
                     MNVAGTLLNSALIYAGNNLKLFTDRLHNQHGDILAGNSLWVQKDASGGANTEIINTSG
                     NIETHQGDIVVRTGHLLNQREGFSATTTTRTNPSSIQGMGNALVDIPLSLLPDGSYGY
                     FTREVENQHGTPCNGHGACNITMDTLYYYAPFADSATQRFLSSQNITTVTGADNPAGR
                     IASGRNLSAEAERLENRASFILANGDIALSGRELSNQSWQTGTENEYLVYRYDPKTFY
                     GSYATGSLDKLPLLSPEFENNTIRFSLDGREKDYTPGKTYYSVIQAGGDVKTRFTSSI
                     NNGTTTAHAGSVSPVVSAPVLNTLSQQTGGDSLTQTALQQYEPVVVGSPQWHDELAGA
                     LKNIAGGSPLTGQTGISDDWPLPSGNNGYLVPSTDPDSPYLITVNPKLDGLGQVDSHL
                     FAGLYELPGAKPGQAPRETAPSYTDEKQFLGSSYFLDRLGLKPEKDYRFLGDAVFDTR
                     YVSNAVLSRTGSRYINGLGSDTEQMRYLMDNAARQQKGLGLEFGVALTAEQIAQLDGS
                     ILWWESATINGQTVMVPKLYLSPEDITLHNGSVISGNNVQLAGGNITNSGGSINAQNG
                     LSLDSTGYIDNLNAGLISAGGSLDLSAIGDISNISSVISGKTVQLESVSGNISNITRR
                     QQWNAGSDSRYGGVHLSGTDTGPVATIKGTDSLSLDAGKNIDITGATVSSGGTLGMSA
                     GNDINIAATLISGSKSQSGFWHTDDNSSSSTTSQGSSISAGGNLAMAAGHNLDVTASS
                     VSAGHRALLSAGNDLSLNAVRESKNSRNGRSESHDSHAAVSTVTAGDNLLLVAGRDIA
                     SQAAGVAAENNVSIRAGRDVNLVAESAGAGDSYTSKKKKEINETVRQQGTEIASGGDT
                     TVTAGRDITAVASSVTATGNISVNAGRDVALTTATESDYHYLETKKKSGGFLSKKTTH
                     TISEDSASREAGSLLSGNRVAVNAGDNLTVEGSDVVADRDVSLAAGNHVDVLAATNTD
                     TSWRFKETKKSGLMGTGGIGFTIGSSKTTHDRREAGTTQSQSASTIGSTAGNVSITAG
                     KQAHISGSDVIANRDISITGDSVVVDPGHDRRTVDEKFEQKKSGLTVALSGTVGSAIN
                     NAVTSAQETKESSDSRLKALQATKTALSGVQAGQAAAMATATGDPNATGVSLSLTTQK
                     SKSQQHSESDTVSGSTLNAGNNLSVVATGKNRGDNRGDIVIAGSQLKAGGNTSLDAAN
                     DVLLSGAANTQKTTGRNSSSGGGVGVSIGAGGNGAGISVFASVNAAKGSEKGNGTEWT
                     ETTIDSGKTVTINSGRDTVLNGVQVNGNRIIADVGHDLLISSQQDTSKYDSKQTSVAA
                     GGSFTFGSMTGSGYIAASRDKMKSCFDSVAEQTGMFAGDGGFDITVGRHTQLDGAVIA
                     STATPDKNHLDTGTLGFSDLHNEADYKVSHSGISLSGGGSFGDKFQGNMPGGMISAGG
                     HSGHAEGTTQAAVADGTITIRDRDNQKQNLANLSRDPAHANDSISPIFDKEKEQRRLQ
                     TVGLISDIGSQVADIARTQGELNALKAAKEATGETLPANATEKQRQEYLAKLRDTQAY
                     RNEMAKYGTGSEIQRGIQAAMAALQGLAGGNMAGALAGASAPELANIIGHHAGIDDNT
                     AAKAIAHAILGGVTAALQGNSAAAGAIGAGTGEVIASAIAKSLYPGVDPSKLTEDQKQ
                     TVSTLATLSAGMAGGIASGDVAGAAAGAGAGKNVVENNALSLVARGCAVAAPCRTKVA
                     EQLLEIGAKAGMAGLAGAAVKDMADRMTSDELEHLITLQMMGNDEITTNYLSSLHDKY
                     GSGAASNPNIGKDLTDAEKVELGGSGSGTGTPPPSENDPKQQNEKPVDKLNQKQESAI
                     KKIDNTIKNALKDHDIIGTLKDMDGKPVPKENGGYWDHMQEMQNTLRGLRNHADTLKN
                     VNNPEAQAAYGRATDAINKIESALKGYGI"
     gene            complement(10111..11883)
                     /locus_tag="RH057_003562"
     CDS             complement(10111..11883)
                     /locus_tag="RH057_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020231833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShlB/FhaC/HecB family hemolysin
                     secretion/activation protein"
                     /protein_id="HDV3129879.1"
                     /translation="MQRRQNNILTKTSLLSRALSVPCCDMFRRGSPWICYLSLSVFSG
                     FFIPAFSSPAAMLSPGDRSAIQQQQQQLLDENQRQRDALERSAPLTITPSPETSAGTE
                     GPCFTVSRIVVSGATRLTSAETDRLVAPWVNQCLNITGLTAVTDAVTDGYIRRGYITS
                     RAFLTEQDLSGGVLHITVMEGRLQQIRAEGADLPARTLKMVFPGMEGKVLNLRDIEQG
                     MEQINRLRTEPVQIEISPGDREGWSVVTLTALPEWPVTGSVGIDNSGQKNTGTGQLNG
                     VLSFNNPLGLADNWFVSGGRSSDFSVSHDARNFAAGVSLSYGYTLVDYTYSWSDYLST
                     IDNRGWRWRSTGDLQTHRLGLSHVLFRNGDMKTALTGGLQHRIIHNYLDDVLLQGSSR
                     KLTSFSVGLNHTHKFLGGVGTLNPVFTRGMHWFGAESDHGKRGDLPVNQFRKWSVSAS
                     FQRPVTDRVWWLTSAYAQWSPDRLHGVEQLSLGGESSVRGFKEQYISGNNGGYLRNEL
                     SWSLFSLPYVGTVRAVAALDGGWLHSDRDDPYSSGTLWGGAAGLSTTSDYVSGSFTAG
                     LPLVYPDWLAPDHLTVYWHVAVAF"
BASE COUNT         2501 a         3620 c         3150 g         3057 t
ORIGIN      
        1 taagttgcct tatttgggta aaggtacttt tctgatcttt tagatcttct ccttttatcg
       61 ttgataaagc tgcaattagt tcaccatcgt aatattcacc cgctaacggc tctttagtta
      121 gaacttccaa cactcttggc atcaactgat caatacataa attttgtcgg atagcgcggc
      181 aaagatcttc cactgttaac ttttcaagag gcacatctat gatacgttcg aaccagagtt
      241 caagcggtga ttgttgctca ggctcttttg tcatattgat gtttccaatc aatttacgta
      301 aggtaatcat attccatatc ctttcaaggc tgattctatt ttattaatag catctgttgc
      361 tctgccatac gcagcctgag cttcaggatt gttgacgttt ttcaacgtat ccgcatgatt
      421 tcttaatcct ctgagcgtat tttgcatttc ctgcatatga tcccaatatc ctccattctc
      481 tttaggaact ggcttaccat ccatatcctt gagagttcca ataatatcat gatctttcag
      541 agcatttttt atagtgttat cgatcttctt aatcgcactt tcttgcttct gattaagctt
      601 atctacaggt ttttcatttt gctgcttagg atcattttcc gatggtggtg gtgtaccggt
      661 tcctgagccg gaaccgccca gttctacttt ttccgcatcg gtcagatctt taccgatatt
      721 cggattcgag gcagccccgg aaccgtactt atcatgcaac gaactgagat aattagtagt
      781 gatctcatca ttacccatca tttgcagggt aatcagatgc tccagttcat cggaggtcat
      841 cctgtcggcc atatccttga ctgccgcccc ggcaagcccg gccatgcccg ctttcgcccc
      901 gatttctagc aactgctctg caactttagt cctgcaaggt gctgcgaccg cacagcctct
      961 ggcaaccaga ctcagcgcat tattctcaac aacgttcttc ccggcaccag ctccagcagc
     1021 cgcgccagcc acatcgccac tggcaatgcc gccggccata cccgctgaca gcgttgccag
     1081 cgtgcttaca gtttgcttct gatcttctgt cagtttcgac ggatctacgc ccgggtagag
     1141 gcttttcgca atggctgacg cgatcacttc gccagtaccc gcgccaattg cgcctgctgc
     1201 cgcactgttg ccctgaaggg ctgctgtcac accaccgaga atggcatggg caatggcttt
     1261 tgccgctgta ttgtcatcaa tacccgcgtg atgaccgatg atgttcgcca gctccggcgc
     1321 tgaagcacct gccagcgcgc ctgccatatt gccgcccgcc aggccctgaa gcgctgccat
     1381 cgcggcctgg ataccccgct gaatttcact accagtgcca tacttcgcca tttcattcct
     1441 gtaagcctgc gtgtcacgca gtttcgcaag atattcctga cgttgtttct ctgttgcatt
     1501 agcaggaagt gtttcgccgg ttgcttcctt cgcggccttc aacgcattca gttctccctg
     1561 tgtacgcgca atatccgcca cctgactgcc tatgtcactg ataagcccca ctgtctgcag
     1621 acgcctctgc tctttctcct tgtcaaatat cgggctgata ctgtcattag cgtgcgcagg
     1681 gtcacggctc aggttcgcca gattctgctt ctgattgtcc ctgtcccgga tggtgattgt
     1741 gccatctgcc actgcggcct gagtcgttcc ttccgcatgt ccgctgtgac ctccggcgga
     1801 tatcatgcca cccggcatgt taccctgaaa tttatccccg aagctgccac caccgctcag
     1861 actgattcca ctgtgactga ctttataatc cgcttcgttg tgaaggtcac tgaaccccag
     1921 cgttccggta tccaggtggt ttttatccgg tgtggcagtg gaggcaatca ccgcaccatc
     1981 cagttgggta tgtctgccca ctgtaatgtc gaagccacca tcaccggcaa acattccggt
     2041 ttgttcagca acggagtcaa agcagctctt catcttatcc cgggaggcag cgatgtaacc
     2101 tgagccggtc atggagccaa aggtaaaact gccgccggca gccacgctgg tctgtttact
     2161 gtcgtactta ctggtgtcct gctggctgct tatcagcagg tcgtggccca catcggcgat
     2221 aatcctgttg ccgttgacct gaacaccgtt cagtaccgta tcccgaccac tgttgatggt
     2281 gacggtttta ccgctatcta ttgtggtttc agtccactca gtaccgttac ctttctcgct
     2341 gccttttgcc gcattaacgc tggcaaagac gctgataccg gcaccgttac caccggcacc
     2401 gatactgaca cccacgccac cgccactgct gctgttcctg cccgttgttt tttgtgtgtt
     2461 tgcagcgcca ctcaacagaa catcattcgc ggcatccagg cttgtgttac caccggcctt
     2521 aagctggctt cctgcaatca caatatctcc gcggttatcg cccctgtttt tgccggttgc
     2581 gacaacagac agattattcc cggcattcag cgtactgccg gatactgtgt cactttcaga
     2641 atgttgttgt gatttcgatt tctgggtggt aagcgacagg ctgactcccg tcgcattcgg
     2701 gtcaccggtt gcggtggcca ttgccgcagc ctgtccggcc tgcacaccag acagcgctgt
     2761 ctttgtggcc tgcagggctt tcagacggct gtcactgctc tccttcgtct cctgtgcact
     2821 ggtgaccgca ttattgatgg cactgcccac cgtgccggaa agggcaaccg tcagcccgct
     2881 tttcttctgc tcaaattttt cgtccacagt acgacggtca tgccccgggt caaccactac
     2941 actgtcaccg gtaatgctga tatcccggtt cgcaatcaca tccgaaccgc tgatatgagc
     3001 ctgtttgccc gcggtaatac tgacattacc ggcagtggag ccgatggtgc tggcactctg
     3061 actctgcgtt gtcccggcct cgcgccggtc gtgcgttgtc ttactgctgc caatggtgaa
     3121 accaataccg ccggtaccca tcagaccgga tttcttcgtt tccttaaagc gccaggacgt
     3181 atctgtattg gtggcagcaa gaacatcaac atggttaccc gccgccagtg acacatcccg
     3241 gtcagccacc acatccgaac cctctaccgt caggttatca ccggcgttaa cggccacgcg
     3301 gttccccgac agcagggagc ctgcttcacg ggaggcactg tcctcactga tggtgtgggt
     3361 ggttttctta ctgagaaaac ctccgctttt tttcttcgtt tccagatagt gatagtcact
     3421 ttctgtcgcc gtggtcaggg caacatcacg accggcattc acgctgatat tgccggttgc
     3481 ggtaacggat gacgcaacag cggtgatatc ccgtcctgcg gtgacggtgg tgtcaccacc
     3541 gctggcaatt tccgttccct gctgacggac tgtctcgtta atctctttct ttttcttcga
     3601 cgtatagctg tcacctgcgc cggcagactc tgccaccagg ttcacatcac gcccggcccg
     3661 gatggacacg ttattttccg cagccacacc ggcagcctga ctggcaatat cacgaccggc
     3721 aacaaggagg aggttatcgc ccgccgtcac cgtggacact gctgcgtggc tatcatgact
     3781 ttctgacctg ccgttgcgac tgtttttgct ttccctgact gcattcagac tcaggtcgtt
     3841 acctgcagaa agcagggctc tgtgcccggc agaaacagag gatgccgtga catccagatt
     3901 atggcctgca gccatcgcca ggttaccgcc ggcgctgatg ctgctgccct gtgaggtggt
     3961 ggatgatgaa ctgttgtcat cagtgtgcca gaaaccggac tgacttttgc tcccgcttat
     4021 cagggttgcg gcaatgttga tatcattacc cgcagacatt ccaagggttc caccggacga
     4081 gaccgttgcc ccggtaatat caatgttttt ccctgcatcc agtgaaagtg aatcagtgcc
     4141 tttaatggtc gcaaccggac cggtgtccgt accgctgaga tgcacaccac catatcggct
     4201 gtcactgccc gcattccatt gctgacgccg ggtgatattg ctgatgttgc cactcacgct
     4261 ttccagttgt acggttttac cgctgatgac tgagctgata ttgctgatat ccccgatggc
     4321 gctcaggtcc aggctaccgc ccgcgcttat cagccccgca ttcaggttgt cgatatagcc
     4381 ggtactgtcg agcgagaggc cgttttgtgc gttgatgctg ccgccgctgt tggtgatatt
     4441 gccacccgca agctgcacgt tgttcccgct gataacgctg ccgttatgca gggtgatatc
     4501 ttccggcgac agatacagtt tcgggaccat gactgtctgt ccgttgatgg tggctgactc
     4561 ccaccacagt atgctgccgt caagctgagc aatctgttca gctgtcagcg ccacaccaaa
     4621 ctctaatccc agtcctttct gttgtctggc cgcgttatcc atcagatacc gcatctgttc
     4681 cgtgtctgaa cccagtccgt tgatataacg tgaacccgtc cggctcagca ccgcgttact
     4741 gacataccgg gtatcaaaga ccgcatcccc caggaaacga taatcttttt ccggtttcag
     4801 cccgaggcgg tcaagaaaat acgatgagcc cagaaactgt ttttcatcgg tatacgacgg
     4861 agccgtttca cgtggcgcct gacccggctt cgctccagga agctcataca gtccggcaaa
     4921 caaatggctg tccacctgtc cgagaccatc cagtttcggg tttaccgtaa tcagatacgg
     4981 actgtccggg tccgtggacg gaaccaggta tccattgttg ccggaaggca gtggccagtc
     5041 atcactgata ccggtctgac cggtcagtgg cgaacctccg gcaatatttt tcagggcacc
     5101 tgccagttca tcgtgccatt gcggagagcc aaccaccacc ggctcatact gctgcagcgc
     5161 tgtctgtgtc agactgtctc cgccggtctg ctgacttaac gtattcagta ccggtgcaga
     5221 gaccaccgga ctgacactac ctgcatgtgc agtggttgtt ccgttattga tactgctggt
     5281 aaaacgggtc ttaacatccc cgcccgcctg aataacggaa taatacgtct taccgggcgt
     5341 gtaatctttt tcccggccat ccagtgaaaa tctgatggta ttgttttcaa attccggtga
     5401 cagcaggggc agtttatcca gagagcctgt ggcatagcta ccgtaaaacg ttttcgggtc
     5461 gtagcggtat accagatatt cattctctgt ccccgtctgc cagctctgat tgcttaactc
     5521 tctgcccgaa agtgcgatat ccccattcgc caggataaat gacgcccggt tttccagtcg
     5581 ttcagcctca gcagaaagat tacgccctga cgcaatgcgg cctgccggat tatcagcacc
     5641 ggttactgtt gtgatgttct ggctgctgag aaagcgctgt gtggcactgt cagcaaacgg
     5701 cgcgtaataa taaagcgtat ccattgtgat attgcatgcc ccgtgcccgt tgcagggcgt
     5761 accgtgctga ttttcaactt cacgggtgaa atagccatag ctgccgtcag gaagaaggga
     5821 aaggggaata tcaaccagag catttcccat tccctgaatg gatgaggggt tagtccgggt
     5881 tgttgttgtg gcagaaaatc cctcccgctg gttcagaagg tgcccggttc ttacaacaat
     5941 atcgccctga tgcgtctcaa tattcccgga ggtattgata atctctgtgt ttgcaccgcc
     6001 ggaagcatcc ttctgtaccc acagactgtt gccggccagg atatcaccat gctggttgtg
     6061 cagacggtct gtaaacagct tcaggttatt ccccgcataa atcagcgcac tgttcagcag
     6121 ggtaccggcc acattcattg tcagactgcc tgccgtgccg gtaaaaccac tgatggtgat
     6181 atcactccgg ctgttcagac tcacatcgcc accggcctga agtgaacccg gtgcgttaag
     6241 gaaaagacgc tgtgcgctga aaacactgtt gcctttaccg gcagtcagcg ttccattgtt
     6301 ggtgaatgcc tctccggcac cgagcaccat ggcatcaccc tgcatgacac cgccgttggt
     6361 aatggcattt tgcgacgtga cggaaagggt tttccctgcg gccagggtac catgattcgt
     6421 gagggcagca atcagtttca gtgtgacatc accggtggcc accacctgac cctgaccact
     6481 gaagtcctga gcgtcaagca gcaggttgcc tgcactgtac agccgccctg taccattttg
     6541 cagcagtgaa ctgcccttga cgccaagccc ggaggttccc agcagggtac cgctgttgct
     6601 gaatgtgtgg taattcacca gcaggtccgc accctgaagc gtaccgctgt tattcagcga
     6661 ggttccttta acgtcggtac tgccggtggc aagtacgcgt ccgccgttga cagcattcac
     6721 cacatccagc agcagggtga cagcctgtac caggccgctg ccggtgttcg ccagcgcctg
     6781 cgcagtcagc gtcaggttac caccggagag gattttgccg tcgttctgca gacggtcagt
     6841 ggcgttcagg gaagctccgc caccaccctg tattgtgccc tggttactca gggtcgttgt
     6901 gctgacattc agtgcattcc ggctcagcag aacaccaccg gaacggttgt tcacgccacc
     6961 ggagactgcc agcgtcagcg tttcgccctg cagatgcccg ccgtttgtga gttgtcctgc
     7021 cgtgatggtg gtggcatttc cctgtaattg cccgtcgttt gtgacactgt ctgccttcag
     7081 cgtcagcaca cctgcactga gcagttttcc gctcgcgtga ttgttcagcg tctgattcac
     7141 catgagcgtg agagcatcca caccggtgat gtcacccgca ctggtcagtg agttcgcctt
     7201 cagggtcaga ttttttgcaa tccattgtcc gctgttgctt aaattcagtg cactgagcgc
     7261 catttcaccg ttcgaggtga ctttgctgcc tgctgtgctg acaagctcac ccgtcagacg
     7321 tgcagtcagg ctgtcagccg cctggatcgc cccgctgttt gccagactgt ctgaggtgat
     7381 cagcacccgt ttgccctgcc agtgtccgga actggtaata ctgcctgcgg taattgtcag
     7441 atcgccgctg gtcagcagtg aacctccgtt attcatcagc gcaggttgag gggatgccat
     7501 acgggcggca agcgtcagcg cggcgatccc ggtgagcgtg ccactgttgg tgacaccgtt
     7561 ctggcgaatc gtgacatggt taccctggac agtgccgctg ttatccagtg aattaccatc
     7621 aagggagagc gtgccggccg aaagcagact gccccggttg tccgtggtgg ctgctttcag
     7681 cgtggtgtca ccctggctca tgatatcgcc ggtactggtc aactgaccgc ttgccgaagc
     7741 ggtaaggtta ccggttgcca gcacggaacc actgttcgcc cagttgtccg cctgcacggt
     7801 gagattctgt ccctgcgtgg tccctgcagt attcagtgtt ttaccccgga gggagaggtc
     7861 gcccgccgtc agccagcgtc cgttacggcc ctgtgagagg gtgtcgcctg caagcgccag
     7921 tgcaccggcg ccctgcaaca ggccgtcgcc atccagcgtg gtcgccctga cgttcagcgt
     7981 gtcagcgatg atttttcccg gattgctgag ggaggcagtg tttaacgtta aaccattatc
     8041 accggtgata agcccgctgt tgcggatgtc cggtatatcc agcgtcaggt ctgcagcact
     8101 gtacagcgtg ccgttctgct gattatcaag cctctgtgtg ttaacggtga gtgaggcctc
     8161 cccctgcaac agaccgctgt tggtcagggt ctgtgactgt gtattcaggg cggaaccaac
     8221 aagtacgcca ctgctggtca gttccggcgc actgaggctg agcgacgggg cactgctttt
     8281 cccgctgtgg gtgagctttt cactggcgtt caccaccatg gtctgttgtg ctgcctgcgt
     8341 acctgcaaga cgtgcatctc tggcgttgat gctgagattt ttgccgctct gaagttgtgc
     8401 gcccgctgcg gtactcagtt tgtctgcctg aacccggagg gtgtcaccgg cactggtttc
     8461 cccgtccagc gccactgttg tcacattcag cgtcatcgca gcatcgctgt gggtgaccga
     8521 ttttttaccg gagctcagcg tctgcgcact gaccgtcagc cctttgccgc cggacagcac
     8581 accgttctgt gtcacatcct gcgccttcag caccagtaca tcatcgctca ccagcgagcc
     8641 tgtactggtc agtttcccgc tggccgtgat atccactttg cccttcgcgc cagtgcgtcc
     8701 actctggtta aagtcgcggg tattcacggt caggggaccg ccactgagca gggagccact
     8761 gttgctgagc gttgtactgc cgagcgtcag ggaagccccc tgaacagcac cactgttatt
     8821 cagcgtgccg gcatcgagtc ccgcatgacc tttcgccagc aatattccgt cctgtgtcag
     8881 cgtggtggcg ctggccgtga gattctgccc ggcggttatc tgtccctggg ttgtcagcgt
     8941 gtcactggcg tcagtcacaa tatcgcgggc cgcgttaatc tggctggcgg tatcctgtgt
     9001 gatgtttttc gcggcaagcg ttacatcccg gccggcagtc agtttttcat tctgttgagt
     9061 gattctgccg ccggcggtca ggctgaggtc cttgtcgctg ttaagcgtgc cattgctgag
     9121 aacgatatcg ctccggctgc tgacgctcag gttacctccc gctttatggt cgccggttaa
     9181 ggtgacgccc tgaccttttg cagtgacggc ccccgtggcg agactgttgt tgacagtcag
     9241 tctgccgctg gcatccaggg tgatatcgcc atcgcgggca taaaggttac caagattaac
     9301 cccgacacca ctttcagtgg aggtcagatg aatacgcctg gcgtacattc caccgagcgc
     9361 gccggtatca acggcaactt tcggcacatc accttcgcct ttcagggcac tgacacgacc
     9421 atctgcagtt acacggttag cgcctgcagt gacagttaaa tccttcgcat gaagcgcggc
     9481 attcacttcc gttgcacggg caataatgga tacggcatcg ctccgggtgc cgtccaggcc
     9541 cgcgccattg atggtgatac tgccttcagt cacctccagc gcctgcaggc tgccgtcggc
     9601 attcatcaca ggtctgcccg tggtgagcgt cgcgtgcggc gtgttgataa aaccacagcc
     9661 gtcacaggtg ataccatacg ggttggcaac catcacattc gccgctttgc cggccacttc
     9721 cgtatagccc tgcagcagtg aacgcttacc gccggtcact tcgttgatga tacccttcgc
     9781 ttccccgccc gctttcaggt tcgggttatt ctgtatcagt ccaccaagct gcgtcggatt
     9841 aagcttaccg gtggcattat tgagaatcag cccttccttc ccgacgttgt aatccgtaaa
     9901 ccggttatgc gaaatcccgg ccccgttcgg cgtggcaatg ttcacgaccg gcacaccatt
     9961 tgccgcttta tccattccgg ccccgttttg tggggtgatg acggccccca cagccggtaa
    10021 caacggctgc ccggcgataa tcgcactgac caggtaactc agcaggcggt aagtgaagtg
    10081 aacgggaggc tgattcatgg taataatccc ttaaaacgcg acggcaacgt gccagtaaac
    10141 cgtgagatgg tcaggggcaa gccagtccgg gtaaaccaga ggcagcccgg cagtgaacga
    10201 accggaaaca tagtcactgg tggtgctgag cccggcagca ccaccccaca gcgtgccgga
    10261 cgagtacggg tcatctctgt cagagtgcag ccagccgccg tccagtgcag ccactgcacg
    10321 gacagttccc acatatggca gggagaacag agaccaggac agctcatttc gcagataacc
    10381 gccgttatta ccggagatat actgctcctt aaagccacgc actgaactct cacccccgag
    10441 gctcagttgt tccacaccat gaagacggtc cggtgaccac tgggcataag cgctggtcag
    10501 ccaccacacc ctgtccgtga cggggcgctg aaaactggca ctcaccgacc atttccggaa
    10561 ctgatttacg ggcaggtctc cccttttccc gtggtcgctt tctgcgccga accagtgcat
    10621 cccccgtgtg aataccggat tcagtgttcc gacacccccc agaaacttgt gtgtgtgatt
    10681 cagcccgaca gaaaatgaag tgagtttacg gctgctgccc tgaagcagaa catcatccag
    10741 ataattgtga ataatgcggt gctgcagacc tccggtcagt gctgtcttca tgtccccgtt
    10801 acggaacagg acatgcgaca gtcccagccg gtgagtctgc aggtctcccg tggaacgcca
    10861 ccgccagccc cggttatcaa tggtgctgag gtagtcactc catgaatacg tataatccac
    10921 cagggtatag ccatacgaca gactgacacc ggcggcaaaa ttcctcgcat catgtgacac
    10981 cgaaaagtca ctgctccgtc ccccgctgac aaaccagttg tcagccagcc ccagcggatt
    11041 attaaaggaa aggacaccat ttaactgccc cgtaccggta ttcttctgcc cgctgttgtc
    11101 gatacccacg ctccctgtga cgggccattc cggcaatgcc gtcagtgtca ccaccgacca
    11161 tccctcacgg tcaccgggcg atatttcaat ctgtaccggc tccgtacgca gacgattaat
    11221 ctgctccatc ccctgctcaa tatcccgcag gttcagaacc ttcccctcca ttcccgggaa
    11281 aaccatcttc agggtgcggg caggaaggtc agcgccttcc gcccggattt gctgcagcct
    11341 gccttccatg accgttatgt gcagtacgcc ccctgaaagg tcctgctctg tcagaaaggc
    11401 ccggctggtg atatatcccc ggcgtatata gccgtccgtc acggcatccg tgaccgcagt
    11461 caggccggtg atattcagac actgattcac ccacggtgcc accagtctgt cggtttctgc
    11521 agacgtcagt cgggtggccc cactgacaac aatgcgtgac accgtaaagc aggggccttc
    11581 agtaccggca gacgtttccg gagacggcgt gatagtcagc ggcgcactgc gctccagcgc
    11641 atcacgctga cgctggtttt catccagcaa ctgctgctgt tgctgctgaa ttgcactgcg
    11701 gtcacccgga gacagcatgg ctgccggaga cgaaaatgcg gggataaaaa aaccagaaaa
    11761 aacggagagg gagagatagc atatccacgg agagccgcgc cggaacatat cacaacaggg
    11821 gacagacaac gcacgggata aaagggacgt tttcgtcaga atgttgttct gccggcgctg
    11881 catgttctcc ccgtattctg gcctgtgtga tgccagtgta aattcatgtc aaaaagtgtc
    11941 agcgagaaaa tttacacctg aaaaagtctg tcatgaaaca gttttatgac aagacatcaa
    12001 aaaaatgtca aaaacgtgat ctttcttcag cttttcagaa tattgagtac caggaagtca
    12061 atatcaattc tgggacacat atgcatttac cattaaaacc gggcacaaca agctgttcag
    12121 tctcaatcca taccccgtgt tttatcaata tcagaataag aatatctgcg gataaataaa
    12181 aaaggaatgt ccagggagct taccgcagag ggctctccac ctcacaaacc atttacaaaa
    12241 atgataccgg catatctttg gtccctgtca caaccagaca ttgacaatcc aatataaccc
    12301 caaatatatt tcgggtaaga agtctggg
//
LOCUS       DAPHDW010000131        12147 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.133, whole genome shotgun sequence.
ACCESSION   DAPHDW010000131 DAPHDW010000000
VERSION     DAPHDW010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12147
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.133"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            231..1001
                     /locus_tag="RH057_003563"
     CDS             231..1001
                     /locus_tag="RH057_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HDV3129880.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNKGGWDGAVITFFASTAAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            992..1465
                     /locus_tag="RH057_003564"
     CDS             992..1465
                     /locus_tag="RH057_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HDV3129881.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGAI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISAVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1572..2111
                     /gene="dnaT"
                     /locus_tag="RH057_003565"
     CDS             1572..2111
                     /gene="dnaT"
                     /locus_tag="RH057_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HDV3129882.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVATEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2114..2851
                     /gene="dnaC"
                     /locus_tag="RH057_003566"
     CDS             2114..2851
                     /gene="dnaC"
                     /locus_tag="RH057_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HDV3129883.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            2900..3394
                     /locus_tag="RH057_003567"
     CDS             2900..3394
                     /locus_tag="RH057_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HDV3129884.1"
                     /translation="MMKTVKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3648..5939
                     /gene="opgB"
                     /locus_tag="RH057_003568"
     CDS             3648..5939
                     /gene="opgB"
                     /locus_tag="RH057_003568"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HDV3129885.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIAAFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRDDKPQQETLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDRCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTMWKGKTAFKDTVIDMARYKSNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6078..7100)
                     /gene="lgoD"
                     /locus_tag="RH057_003569"
     CDS             complement(6078..7100)
                     /gene="lgoD"
                     /locus_tag="RH057_003569"
                     /EC_number="1.1.1.414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
                     /protein_id="HDV3129886.1"
                     /translation="MSTMNVLICQQPKELVWKQREIPIPGDNEALIKIKSVGICGTDI
                     HAWGGNQPFFSYPRVLGHEICGEIVGLGKNIADLKNGQQVAVIPYVACQQCPACKSGR
                     TNCCEKISVIGVHQDGGFSEYLSVPVANILPADGIDPQAAALIEPFAISAHAVRRAAI
                     APGEQVLVVGAGPIGLGAAAIAKADGAQVVVADTSPARREHVATRLELPLLDPSAEDF
                     DAQLRAQFGGSLAQKVIDATGNQHAMNNTVNLIRHGGTVVFVGLFKGELQFSDPEFHK
                     KETTMMGSRNATPEDFAKVGRLMAEGKITADMMLTHRYPFATLAETYERDVINNRELI
                     KGVITF"
     gene            7239..8153
                     /locus_tag="RH057_003570"
     CDS             7239..8153
                     /locus_tag="RH057_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3129887.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSHLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPGETFSELQLARAAGVSPVLVREYLLKFGRYNLIHSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8368..9729
                     /locus_tag="RH057_003571"
     CDS             8368..9729
                     /locus_tag="RH057_003571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3129888.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHNFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLSFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IIAGMFCSAAFTLVVPQATTSMTAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(9778..11442)
                     /gene="tsr"
                     /locus_tag="RH057_003572"
     CDS             complement(9778..11442)
                     /gene="tsr"
                     /locus_tag="RH057_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HDV3129889.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQSTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGIAFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAAREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEIASASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPAAPRKMAVADSEENWETF"
     gene            complement(11561..12007)
                     /gene="hpaR"
                     /locus_tag="RH057_003573"
     CDS             complement(11561..12007)
                     /gene="hpaR"
                     /locus_tag="RH057_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HDV3129890.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEAIPGDNE"
BASE COUNT         2949 a         3197 c         2958 g         3043 t
ORIGIN      
        1 atcctgattc gtaaaggcca aacaataaat tcattatagt gataatattc atgtgatagt
       61 ttgtttgcgg cgagagataa ttcgcttttt atcaccgagc ttgtcccgat aaccaggatt
      121 aatcagcgca attttgatct taacctaccg caaaacaacc gtgcttcatg atacaaatgg
      181 attctcatta tctgaacaat acgcagcacg tctatgacaa agggcgagtt atgcaaactg
      241 agcaacagcg agccgtaaca cggctttgta tccagtgtgg attatttctt ttgcaacatg
      301 gtgcggaaag cgcgttggtt gatgagcttt cctcacgact gggtcgggcg ttggggatgg
      361 acagcgtcga aagttctatc tcttcgaacg ccatagtgct gacaaccatt aaagatgggc
      421 aatgcctgac atcgacacgt aaaaatcacg atcgcggcat taatatgcat gtggtgactg
      481 aagtccagca cattgtgatt ctggcggagc accatctgct ggattacaaa ggcgtagaga
      541 aacgatttag ccaaattcag ccattacgtt acccaagatg gctggtagcc ttaatggttg
      601 gcctttcttg tgcctgtttc tgtaaactca ataaaggtgg ctgggatggt gccgtcatca
      661 ccttctttgc cagtacggcc gcgatgtata tccgccagct gctggcacaa cgtcatcttc
      721 atccacaaat caacttttgc cttaccgctt tcgccgccac caccatttcc ggattgcttt
      781 tgcaacttcc cactttcagc aatacaccca ctatcgcgat ggccgccagc gttctgctgc
      841 tggtgccggg atttccgttg attaatgccg tcgccgatat gtttaaaggc cacatcaata
      901 ccggactggc acgctgggcg atcgccagtc tgctgacact ggctacctgc gtcggcgtag
      961 tgatggcact gacgatttgg gggctacgcg gatgggtgtg atcgaatttc tgttagcgtt
     1021 ggcgcaggat atgatcctcg ccgccattcc tgcggtcggc tttgcgatgg tgttcaacgt
     1081 tcccgttcgg gcgttacgct ggtgtgcgct gcttggcgcg attggtcatg gttcacgaat
     1141 gatcttaatg accagcgggc tgaatattga gtggtcaact tttatggctt ctatgctggt
     1201 cggcaccatt ggtattcaat ggtcgcgctg gtatctggcg catccgaaag tgtttaccgt
     1261 ggcggccgtt atccctatgt tcccgggcat atcggcttat accgcaatga tttcggcggt
     1321 aaaaatcagc cagttaggtt acagcgaacc gttgatgatt accctgttaa ccaactttct
     1381 tacagcttca tcgattgttg gtgcgttatc catcggtctt tccattcctg gattatggtt
     1441 gtaccgcaag cgccctcgcg tataaaattg cctcgcttaa gcacacggat gagagacagc
     1501 ctcctctcct ccgtgtgtta ctataaaagt tatctccctt ctcgttcatc gttccatatt
     1561 tgagaaacag tatgtcttcc agagttttga ccccggacgt cgttggtatt gacgccctgg
     1621 tacacgatca ccaaaccgtt ctggcaaaag ctgaaggcgg tgtggttgcc gtatttgcta
     1681 acaatgcccc ggcgttttat gccgtcacgc ctgcacgcct ggctgaactg ctggcgctgg
     1741 aagaaaagct ggcgcgtccg ggaagcgatg tcgctctgga cgatcaactc tatcaggaac
     1801 cgcaagccgc tcccgttgct gtccccatgg ggaaattcgc catgtatccg gactggcaac
     1861 ccgatgccga ctttatccgc ctggcggcgc tatggggcgt ggcgctaaga gagccggtgg
     1921 ccaccgaaga actggcctca ttcattgcct actggcaggc ggaaggtaaa gtctttcacc
     1981 atgtgcagtg gcaacaaaaa ctggcgcgca gcctgcaaat cggtcgtgcc agcaacggcg
     2041 gactgccgaa acgagatgtg aatacggtca gcgaacctga cagccaaatt ccaccaggat
     2101 tcagagggta acgatgaaaa acgttggcga cctgatgcaa cgcctgcaaa aaatgatgcc
     2161 tgcccatatc aaacctgcat tcaaaacggg tgaagagcta ctggcctggc agaaagaaca
     2221 aggagcgatc cgctccgccg ctctcgaacg tgaaaatcgg gcgatgaaaa tgcagcgcac
     2281 ctttaaccgc tccggtattc ggccactgca tcagaactgc tcctttgaga actatcgcgt
     2341 tgagtgtgaa gggcagatga atgcgttaag caaagcgcgc cagtacgtcg aagagtttga
     2401 cggcaacatc gccagcttca tcttttctgg taagccagga accggcaaaa accatctggc
     2461 ggcggcaatc tgcaacgagc tgctgctacg cggtaaatcc gtattgatca tcaccgtggc
     2521 cgatattatg tcggcgatga aagatacctt caggaatagc ggtaccagtg aagaacaact
     2581 gcttaacgat ctgagcaacg ttgatctgct ggtgatcgat gagatcggcg tgcagaccga
     2641 atcgaaatac gaaaaagtga tcatcaacca gatcgtcgat cgccgctctt cttccaaacg
     2701 cccaaccggg atgctgacca acagtaatat ggaagagatg accaagttgc tgggcgaacg
     2761 cgtgatggac cgtatgcgcc tgggtaacag tttgtgggtg atcttcaact gggatagcta
     2821 ccgtagccgg gtaacaggta aagagtatta atccccgttt ctggcggtat actgcgacac
     2881 aattccacct tgagtaatga tgatgaaaac tgttaaacat cttctgtgct gtgccattgc
     2941 cgccagcgca ttaatttcca ccggggtgca tgctgcgtcc tggaaagatg cgctctccag
     3001 cgccgccagc gaacttggca accaaaacag cacgacacag gaaggcggtt ggtcgctcgc
     3061 gtcattaact aacttgctta gcagcgggaa ccaggcctta agcgcagata acatgaacaa
     3121 cgccgcaggc attctgcaat actgcgcgaa gcaaaagctg gcgtcggtaa ccgatgccga
     3181 aaacatcaag aaccaggtgc tggaaaagct gggcctgaac agtgaagagc aaaaagaaga
     3241 caccaactat ctggatggca ttcagggttt gctgaaaacc aaagatggtc agcaactcaa
     3301 tctggataac atcggaacga ctccgctggc agaaaaggtg aaaaccaaag cctgcgatct
     3361 ggtgttaaag caggggctga acttcatttc ctgaaccgcg aaacgccgcg ttttagctgt
     3421 tacccggctg acgcggctct ctcctgccct tttccctgct cctggacggt tttccccctg
     3481 ttacacggga agcgaccgcg atcaaaaaag tctgtttata aactaattcc taacaacatc
     3541 gcattttttt gacactagaa ttgcagcaat atggcgacgc cattacattg tgcttctcaa
     3601 aaagcttaaa cgttggctaa acggcccggt ttagtgagga tcgcccgttg tcagaactac
     3661 tctctttcgc cctttttctc gcctctgtgc tgatttacgc atggaaagcg ggacgtaaca
     3721 cctggtggtt tgcagccaca ttaacggtgc tggggctatt tgtcgtttta aatatcaccc
     3781 tgtttgccag cgactatttt actggcgatg gtattaacga cgcggttctc tataccttaa
     3841 ccaacagcct gaccggtgct ggcgtcagca aatacatcct gccgggtatc ggcattgtgc
     3901 tggggctgac agcggtgttc ggtgcgctgg gctggatcct gcgccgtcgt cgccatcatc
     3961 cgcaccattt tggttacagc ctgctggcgc tcttactggc gctgggttcg gtggacgcca
     4021 gcccagcatt tcgtcagata acggaactgg tgaaatccca gtcacgcgat ggcgacccgg
     4081 actttgcggc ttattataaa gagccgtcga aaactatccc tgacccgaaa ctcaacctgg
     4141 tttatatcta cggcgaaagt ctcgagcgga cctattttga taacgaagct ttcccggatc
     4201 tcacgcctga actgggcgcg ttgaaaaatg aaggcctgga tttcagccac acgcagcagc
     4261 tgccaggaac ggattacacg attgcgggca tggtggcttc tcagtgcggc ataccgctgt
     4321 ttgccccctt tgaaggcaac gcctccgcct ctgtctccag cttcttcccg cagaacatct
     4381 gtctgggcga tatcctgaaa aactcgggtt atcagaacta tttcgtgcag ggcgcgaatc
     4441 tgcgttttgc cggtaaagat gtgttcctga agtcgcatgg cttcgaccac ttatacggct
     4501 cagaagagct gaaaagcgtg gtggccgacc cgcactatcg caacgactgg ggattctacg
     4561 acgataccgt tctcgatgaa gcgtggaaaa agtttgaaga gctttcccgc tcaggtcagc
     4621 gattctcact gtttaccctg acagtcgata cccatcaccc ggatggtttt atctcgcgta
     4681 cctgtaaccg caaaaaatat gattttgacg gtaagccgaa ccagtcattc agcgcggtaa
     4741 gttgcagcca ggagaatatc gcggcgttta tcaacaaaat caaagcgtca ccgtggttta
     4801 aagataccgt catcgtcgtc tcttctgacc atttagcgat gaacaacacg gcgtggaaat
     4861 accttaataa gcaggatcgc aataacctgt tttttgtcat tcgtgacgac aagccgcagc
     4921 aagagacgct ggcagtgaag cgtaacacga tggataacgg cgcgacggtg ctggacattc
     4981 tcggtggcga taactatctc ggacttggtc gtagcagttt atccgggcag tcgatgtcgg
     5041 aaatcttcct caatatcaaa gagaaaacat tggcgtggaa gccggatatc atccgcctgt
     5101 ggaaattccc taaagagatg aaagagttca ctatcgacca gcagaaaaac atgattgcct
     5161 tctcgggtag ccatttccgt ttgccgctgc tgttgcgggt ttcagacaag cgcgtagaac
     5221 cgctgccgga aagtgaatac tcagcaccgc tgcgtttcca gctggccgat ttcgctccac
     5281 gcgacaattt cgtctgggtt gaccgttgct acaagatggc acaactttgg gctccggaac
     5341 tggcactctc caccgactgg tgtgtctcgc aggggcagct tggcggtcag caaattgtcc
     5401 agcatgttga taaaacaatg tggaagggca aaacggcatt taaagatacg gtcatcgaca
     5461 tggcgcgtta caaaagcaat gtcgatacgc tgaaaattgt tgataacgat attcgctaca
     5521 aagccgacag tttcatcttc aacgtcgccg gtgcgccaga agaggtgaaa cagtttagcg
     5581 ggatttcccg tccggagtcg tggggccgct ggtccaacgc tcagctgggc gatgaagtaa
     5641 aaatcgagta caagcatccg ctgccgaaga aatttgacct ggtgattacc gccaaagcat
     5701 acggcaataa cgccagccgt cctattccgg tacgcgtagg caatgaagaa caaacccttg
     5761 tgctgggcaa tgaagtgacc accaccacac tgcatttcga taacccaacc gatgccgaca
     5821 cactggtaat tgtgccgccg gaacctgtct caaccaacga agggaatatc ctcggacact
     5881 cgccgcgtaa gctcgggatc ggcatggtgg aaattaaagt ggtggaacgt gaaggataat
     5941 tcctgactga tgtgtaggtc gcataaggtg cgacctacac accttctgcg gcctacaaaa
     6001 catcattgag aaacagaaaa aaacagcgcc caacacttcc ccggacgctg catggcaact
     6061 ttaataatct cgccttatca gaaagtaatt acacccttaa tcaactcacg attgttaatc
     6121 acatcgcgct cgtaggtttc tgccagcgtg gcgaacggat agcgatgggt taacatcatg
     6181 tcggcagtga tttttccttc cgccatcagt cgaccgactt tagcaaaatc ttccggcgtg
     6241 gcgttgcggc tgcccatcat cgtcgtttct tttttatgga attccggatc ggagaactgc
     6301 aactcacctt taaacaggcc gacaaatacc accgtgccgc cgtgacgaat caaattcacg
     6361 gtgttattca tcgcatgttg attacctgtc gcgtcgatca ctttctgcgc cagcgaacca
     6421 ccaaactgcg cccgtagctg cgcgtcaaaa tcttcggctg acgggtccag taaaggtaat
     6481 tccagacgcg ttgccacatg ttcacggcgc gccggactgg tatccgccac caccacctgt
     6541 gcgccatcgg ctttagcgat tgccgccgcg cccagaccga ttggccccgc cccgaccacc
     6601 agcacctgct cgccgggagc aatggctgcg cgacgcaccg catgagcgct aatagcgaaa
     6661 ggttcaatca atgctgccgc ctgcgggtca ataccgtctg cgggcaaaat gttcgccacc
     6721 ggcacgctca aatactcact aaaaccgcca tcctgatgca cgccaatgac tgaaattttt
     6781 tcacagcaat tggtacgccc gcttttacac gccgggcatt gctgacaggc aacataaggg
     6841 atcacagcaa cttgctgacc atttttaaga tcagcaatat ttttacccag cccaacaatc
     6901 tccccacata tttcatggcc taaaacacgt ggataactaa aaaatggttg atttccaccc
     6961 caggcatgaa tatcggtacc gcaaatcccg acagacttaa tttttattaa tgcttcattg
     7021 tcacccggaa taggtatctc gcgttgtttc cagactaatt ctttcggctg ctggcaaatt
     7081 aaaacattca tcgtagacat gattcggctc tccatttttt gttgccttag atatcaatga
     7141 aaatgaaaaa tggcagcgga acggaaaatc tgttttgtga aaacacacgc ataaaaatgt
     7201 tttaaatcgg gttttaatgg actcacaagg aggaaaatat gagtcgttca caaaatttac
     7261 gccacaatgt gattaaccag gtcattgatg atatggcgcg gggtcatatc ccttccccac
     7321 ttccatcgca aagtgcgctg gcggaaatgt acaacatcag ccgtactaca gtgcgtcata
     7381 ttctcagcca cttacgcgaa tgcggcgtct taacgcaggt tggaaacgac tatgttattg
     7441 cgcgtaaacc tgaccatgat gacggtttcg cctgtactac cgcttcaatg agtgaacaaa
     7501 acaaagtgtt tgagcaggct tttttcacga tgataaacca gcgccagtta cgcccagggg
     7561 aaacgttttc tgaactgcaa ctggctcggg ccgcgggtgt cagcccggtt ctagtaagag
     7621 aatatctttt aaaattcggc cgttataatc ttattcacag cgaaaagcgc ggccagtgga
     7681 gcatgaagca attcgatcaa tcctacgccg agcagctgtt tgagctacgc gaaatgctcg
     7741 aaacacattc acttcagcac tttctcaacc tgcctgatca tgatccccgc tggctgcagg
     7801 cgaaaaccat gctcgaacgt catcggctac tgcgtgacaa catcggcaat agctttcgca
     7861 tgttctcgca acttgacaga gattttcact ccttactgct ttcagctgcc gacaatatct
     7921 tttttgatca atcgcttgag atcatctcag tcatctttca ttttcactat caatgggacg
     7981 aaagcgatct taaacagcgc aatatcattg ccgtcgatga gcatatgacc attctcagcg
     8041 ctctgatttg ccgcagtgac cttgatgcca cccttgcgct ccgtaaccat ttgaattcag
     8101 ctaaacaatc aatgattcga tccattaatg agaacacgcg ttatgctcat taagtacgga
     8161 ttaaaaacag caaactatta gcgggtgcac ggttttatca aaagataaat accgggaaat
     8221 tcccgcttac ctatgctcac aatcagtcga ctaaaaatgt ttttgtgagc ggtagtaaag
     8281 tcctaaaact ttaacctgcc gttaatgtca accttgcgta atgcacaatt acgtaaacaa
     8341 ttaaaattat aaatacagga gtccggcgtg gaaaaagaaa atatcaccct cgatccgcgt
     8401 tcttcattta ctccatcttc gtcggcagat attcccgtgc caccagatgg attagttcaa
     8461 cgcagtaccc gaattaaacg cattcaaacc accgccatgt tgttattatt ttttgcggcg
     8521 gtaatcaatt atctcgaccg cagttcgctg tcggtagcaa atttaacgat tcgtgaagaa
     8581 ttgggattaa gtgccaccga aatcggcgct ttgctctccg tgttttcact cgcttacggg
     8641 attgcgcaac ttccttgcgg cccactattg gatcgtaaag gcccacgcct gatgctggga
     8701 ctggggatgt tcttctggtc actgttccag gcaatgtctg gcatggtgca caactttacg
     8761 cagttcgtgt tggtgcgtat cggtatgggg attggtgaag cgccgatgaa cccatgcggt
     8821 gtaaaagtca ttaacgactg gttcaacatc aaagagcgcg gacgcccgat gggcttcttc
     8881 aacgcagctt ctaccattgg cgttgccgta agcccaccga ttctggcggc gatgatgctg
     8941 gtgatgggct ggcgcgggat gtttattacc attggtgtac tggggatttt tctcgccatc
     9001 ggctggtata tgctctatcg caaccgcgag cacgtagaac tgactgccgt tgaacaagct
     9061 tatctcaatg caggtagcgt caatgcccgc cgagatccgc tcagttttgc cgaatggcgc
     9121 agcctgttcc gtaaccgtac aatgtgggga atgatgctcg gattcagtgg tatcaactac
     9181 actgcgtggc tgtatctggc ctggcttcct ggttacctgc aaacagccta taacctggat
     9241 ttaaaaagca cagggttgat ggcggctatc cctttcctgt ttggggctgc cgggatgctg
     9301 gtcaacggtt acgttactga ctggctggtc aaagggggaa tggctccgat taaaagccgt
     9361 aagatctgca ttattgccgg gatgttctgt tctgccgcct ttacgctggt cgtaccacaa
     9421 gcgacaacat ccatgacggc ggttctgctg attggcatgg cactgttctg tattcacttt
     9481 gccggaacat cctgctgggg cttgatccac gtcgcagttg cttctcgcat gactgcgtcg
     9541 gtgggcagta tccagaactt tgccagcttc atctgcgcct cttttgcgcc gatcattact
     9601 ggttttattg ttgataccac ccactcattc cgtctggcac taatcatctg cggttgcgtc
     9661 accgcagcgg gggcactggc gtacatcttc ctggttcgtc agccgatcaa cgacccacgt
     9721 aaagattaat ctccttatgc ccgataacat tttgcttatc gggcattttc atggcgatta
     9781 aaatgtttcc cagttctcct cgctatctgc cacggccatt ttacgcggcg cagctggcgt
     9841 cacggttttt accacagccg atgtttcacg ctgctgttgc tgctgttgct gaatccggaa
     9901 cactgccaca gcttcggtca ggcgactggc ctgctcttcc agcgcggcgg cggcagcggc
     9961 agattcttcc accagcgcgg cgttctgttg agttacccgg tccatctcag caaccgctaa
    10021 gccaacctga tcgataccac ggctctgctc atcagaagca gaagcaattt cgcccataat
    10081 gtccgtcacg cgggtcacgg cgctgacaat ctccgccatt gtttccccgg cgctttcgac
    10141 cagcgtagag ccaacatcca ctttccccac cgagtcttca ataaggcttt taatttcacg
    10201 agccgcctga gcgctacgct gggccagatt acgcacttct cccgcaacca ccgcaaaacc
    10261 gcgcccttgc tcacccgcac gcgctgcttc aaccgccgcg ttcaaagcca gaatattggt
    10321 ctggaaggca atgccgtcaa ttacgctgat aatatcggcg attttctgcg aactggtgga
    10381 gatatcgcgc atagtctgca ccacgttatc taccactttg ccgccccgtt gcgccgtttc
    10441 agaagcactt aatgccagat ggctggcctg gcgcgcattc tcggcgttct gtttcaccgt
    10501 tgcggtcagt tgctccatgc tggctgccgt ctcttccagc gaagcggcct gttgctcggt
    10561 gcgcgaagag agatcgttat tgccggtagc gatttcgctg gcaccgctat agatggcatt
    10621 ggccccgttg cgcacatcac cgacggtacg catcagctct ccctgcatat ggcgcaaact
    10681 ctctgccagt tgccccatct cattagagcc atccacctca atcggtttca ccagatcgcc
    10741 gcctgcaata tgacggatgc tgtcaatcag gcgattcatt ggcgctacca gcgaggcttt
    10801 aataccgaac cagacggcga agatgaccgc cagtacgacg atcatcacgc ccaccagaat
    10861 ccacatcgcc tggctgtagg aggcattgtt atcgctgacg gcgatatcat agagccgatc
    10921 gttttgctcc atgtaagcca catactgctt ctcgaaaccg tcttgatatc cctgggtcgg
    10981 ctgatcaaag aactcgttga ttttgcctgc acctaacagt tggatcagct ccgccagcgc
    11041 attgtgataa atatcgtaat tacgtttgat ctctgccgct gcggcggtgc tctgacgcgg
    11101 gtcacgcggc aacgcttcgt aatccgccca gtttttttcc gcctgtttca gcgaaatact
    11161 ggcactctgc atcagctcag caacggttga accgctacca atattattct gatccatcat
    11221 gtagcggata cccgcgcggt tgagggtgtt acgcgtctgc aacaacgcga cccagctgcc
    11281 attcagcgtg gattgctgct ggcgaatggt ttgtaaaaca gtgaaatttt ctttgtcatt
    11341 ctttaaggca ttaaagaaca gaccgcctga tgtcagttgt aaaaggccaa aaacggccaa
    11401 aaccagcagt aagctggtca cgatcttgat acgttttaac atggtttctc tttcctgtgg
    11461 accgatgcaa gattttcggc ctggaaagca aaaactttat gaaaaggtgt aacccgccat
    11521 ccatggaaaa accaggcgct ggttatcaga tactaaaaag ttattcatta tcgcccggga
    11581 tggcttcttg tcgggaattg cccagagcaa taaattcctc taacagatgg gttaactgtt
    11641 gcattttctc ggcagtaaac tgagcttcaa tctgccgata ggcttcttca atctgtgtct
    11701 gagcgcggtt atacagcgcc tgcccctctt tggttagcga gatatagagc ttccgctgat
    11761 cattgatcgg ctttaatcgc aacactaaac cgtcgcgttc catccgcgta aggatcccgg
    11821 tcaggcttgg gcgcaaaatg catgcgcgat acgccagatc gtgaaaatcc attgatgggc
    11881 tttccgccag aatacgcacg atgcgccact gttgctcagt caaattatgc cgtttaacga
    11941 ttgggcgaaa ataactcatc gccgcttctc gcgcctggag caacgcaatg gttagagagt
    12001 cgtgcattat ctttcccctg aaaaattcaa aatcattaat atagaaacag ttaatcatca
    12061 cgttaccggg actgcgctgc ccctgcaatt cttcatttta tcaaaattga gcatgttttg
    12121 ttaacttgct gatatttaat attttat
//
LOCUS       DAPHDW010000132        12106 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.134, whole genome shotgun sequence.
ACCESSION   DAPHDW010000132 DAPHDW010000000
VERSION     DAPHDW010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.134"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            125..559
                     /gene="slyA"
                     /locus_tag="RH057_003574"
     CDS             125..559
                     /gene="slyA"
                     /locus_tag="RH057_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HDV3129891.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            complement(606..1073)
                     /gene="slyB"
                     /locus_tag="RH057_003575"
     CDS             complement(606..1073)
                     /gene="slyB"
                     /locus_tag="RH057_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HDV3129892.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            1347..2456
                     /gene="anmK"
                     /locus_tag="RH057_003576"
     CDS             1347..2456
                     /gene="anmK"
                     /locus_tag="RH057_003576"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HDV3129893.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHST
                     ERRMVLNIGGIANLSLLIPGQPVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAALLPGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANP"
     gene            2554..2883
                     /gene="mliC"
                     /locus_tag="RH057_003577"
     CDS             2554..2883
                     /gene="mliC"
                     /locus_tag="RH057_003577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-type lysozyme inhibitor"
                     /protein_id="HDV3129894.1"
                     /translation="MSMKKLLLICLPVLLTGCSAFNQLVEHMQTATLEYQCDEKPLTV
                     KLNNPRQEVSFVYDNQLLHLKQGISASGARYTDGIYVFWSKGDEATVYKRDRIVLNNC
                     QLQNPQR"
     gene            2942..3598
                     /gene="pdxH"
                     /locus_tag="RH057_003578"
     CDS             2942..3598
                     /gene="pdxH"
                     /locus_tag="RH057_003578"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HDV3129895.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVATVDEHAQPYQRIVLLKHYDEKGMVFYTNLGSRKAHQIENNPRVSLL
                     FPWHTLERQVMVIGKAERLSTLEVMKYFHSRPRDSQIGAWVSKQSSRISARGILESKF
                     LELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQGGEHRLHDRFLYQRENDAWKIDRLAP
                     "
     gene            3727..5001
                     /gene="tyrS"
                     /locus_tag="RH057_003579"
     CDS             3727..5001
                     /gene="tyrS"
                     /locus_tag="RH057_003579"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HDV3129896.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYGVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            5060..5923
                     /gene="pdxY"
                     /locus_tag="RH057_003580"
     CDS             5060..5923
                     /gene="pdxY"
                     /locus_tag="RH057_003580"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HDV3129897.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIAQGPQIVLVKHLARAGYSRDRFEMLLVTADEAWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(5967..6572)
                     /gene="gstA"
                     /locus_tag="RH057_003581"
     CDS             complement(5967..6572)
                     /gene="gstA"
                     /locus_tag="RH057_003581"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HDV3129898.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     AVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLEKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(6678..8180)
                     /gene="dtpA"
                     /locus_tag="RH057_003582"
     CDS             complement(6678..8180)
                     /gene="dtpA"
                     /locus_tag="RH057_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HDV3129899.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPINY
                     RNLLLTIIGVVALIAIATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAIIAVLMLLTAPKLHRMTQDDAADKAAKAAVA"
     gene            complement(8791..9426)
                     /gene="nth"
                     /locus_tag="RH057_003583"
     CDS             complement(8791..9426)
                     /gene="nth"
                     /locus_tag="RH057_003583"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HDV3129900.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     VSVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(9426..10121)
                     /gene="rsxE"
                     /locus_tag="RH057_003584"
     CDS             complement(9426..10121)
                     /gene="rsxE"
                     /locus_tag="RH057_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HDV3129901.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGMGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(10125..10745)
                     /gene="rsxG"
                     /locus_tag="RH057_003585"
     CDS             complement(10125..10745)
                     /gene="rsxG"
                     /locus_tag="RH057_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HDV3129902.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKA
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWITHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(10749..11807)
                     /gene="rsxD"
                     /locus_tag="RH057_003586"
     CDS             complement(10749..11807)
                     /gene="rsxD"
                     /locus_tag="RH057_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HDV3129903.1"
                     /translation="MVFRIASSPYIHNQRQTSRIMLLVLLAAVPGIAAQLWFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGLWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLIFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(11808..>12106)
                     /locus_tag="RH057_003587"
     CDS             complement(11808..>12106)
                     /locus_tag="RH057_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129904.1"
                     /translation="EPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAA
                     VEAAIARAKARKLEQQQTNAEPEEQVDPRKAAVAAAIARAQAKKAAQQKVVNED"
BASE COUNT         3102 a         3028 c         3064 g         2912 t
ORIGIN      
        1 atttctgaaa aatatgtctg acggtaacca aatgcagcaa tacatttgtt ttagcaatac
       61 aattgctgca cactattcta aaagccgcat aatatcttag caagctaatt ataaggagat
      121 gaaattggaa tcgccactag gttctgatct ggcacggttg gtgcgcatat ggcgtgctct
      181 gatagaccat cgcctgaaac cgctggagtt aacacaaacc cattgggtta cgttacacaa
      241 tatccatcag ttacctccag accagtcgca aattcaactg gcaaaagcga ttggcatcga
      301 gcagccatca ctggtccgta ctctggacca actggaagaa aaagggttaa tttcgcgtca
      361 aacttgtgcc agcgatcgtc gggctaaacg tattaaactg acggaaaagg cagagccact
      421 gatcagcgaa atggaagctg ttattaacaa aacccgcgcg gaaatattac atggcatctc
      481 cgcagaggaa ctggagcaac tgattacgct catcgcaaaa cttgagcata atatcattga
      541 gttacaggcc aaagggtgaa atgaaggggg cgtgtggtca gtaacctgac cacacgcaaa
      601 cttatttagc gcggagaaac ggtcacctga ctgccattgc tggccagtac gacacgttgg
      661 cccggagaga aacgagtgtt gccttgtttc tgtaccacca tgatggtatt accatcgtct
      721 ttacgaattt ccagctcgac accctgcgtt ttgttcattg cactctgtac gccctgacca
      781 gctacgccac ctgcaacagc gcctgctgca gtagccagag aacgcccggt tccgccacca
      841 acagtattcc ccaggaaacc accaagaaca gcaccgccaa ttgcaccgat aacgttggaa
      901 tcatcaccgc cctgaatctg taccggacgt acgttaacga tggtgccata gctgacattc
      961 tgtacttgtt tcgcttcaga agcggtataa acatcccctg acagggtgtc gttattaaca
     1021 caaccgacaa gagacagacc taccattgaa acaaccaata cgcgtttaat cattgaaaaa
     1081 tctcctgttc accatgaaac gccacgcaag catccctcat ggtcaaagta tatggcatat
     1141 gtgaatccat ggttacatgt tctaccaaat atgagcaaat cataatgaaa gtaggatcaa
     1201 ccaattataa acaaaacatt catgatgatt attgatttga agataatgtt ttactaccaa
     1261 cggtaaaaat cggtcattcc gcatggtatt cacgaaaagt ttatgtttga gtggtgcgca
     1321 aacgtcagat tgaaaaagga tgaactatga aatcgggccg ctttattggc gttatgtcag
     1381 gcaccagcct tgatggtgtt gatgttgtgt tggcgacaat agatgaacac cgggtcgcac
     1441 agctggcaag cttgagctgg ccgatcccgg tgtctctgaa acaggctgta ctggatattt
     1501 gccagggcca gcagcttaca ctttcgcagt ttggacagct tgatactcaa ctcgggcgac
     1561 tttttgctga tgcggtcaat gccttgctta aggaacaaaa cctgcaggcg agagatatag
     1621 ttgcgatcgg ctgtcacggt caaaccgtct ggcatgaacc gacgggcgtg gcaccacaca
     1681 ctttacagat tggcgataac aatcaaattg tggcacgcac cggaattacg gttgtcggtg
     1741 attttcgccg tcgcgatatt gccttgggag gacaaggcgc accgctggta cctgcgttcc
     1801 atcatgcctt gctggctcac tcaaccgagc gacgaatggt gctcaatatt ggcggcatcg
     1861 ccaatctgtc actgctcatt cctgggcagc cggttggcgg ctacgatacc ggtcctggta
     1921 acatgctgat ggatgcctgg atctggcgtc aggccggtaa accttacgat aaagatgccg
     1981 agtgggcacg ggcgggtaaa gttattctcc cactgctgca aaatatgctc agcgacccat
     2041 atttctcgca acctgcaccg aaaagcaccg gacgcgaata ctttaactac ggctggctgg
     2101 agcgccattt acgccatttt ccgggtgttg atccccgtga tgttcaggcc acactggcag
     2161 aactcaccgc cgtgaccatt tctgaacaag ttttgttgag cggtggctgc gaacgattga
     2221 tggtatgtgg tggaggtagt cgtaatccgc tactcatggc gcgtctggcg gcattactgc
     2281 caggcacaga agtcaccacc accgatgccg ttggcattag tggcgatgac atggaagcat
     2341 tggctttcgc ctggcttgcc tggcggacgc tggcgggatt accaggaaat ctgccttccg
     2401 tcactggcgc aagccaggag acggtactgg gggctatttt ccctgctaac ccgtgatatc
     2461 agagttaact gaatttttct taccgcgtct gccgttacac ttggaacatt agggagggcg
     2521 tattgccctc cagaccagga aagtcttcgg gaaatgtcta tgaaaaaact gttactgatc
     2581 tgtttgccgg tgcttctcac cggctgtagc gcgttcaatc aactggttga gcacatgcaa
     2641 accgctacgc tggaatacca gtgtgatgaa aaaccgttga cggtcaaact gaataatccg
     2701 cgccaggaag tcagttttgt ttacgataat caactactgc atctcaaaca gggcatttca
     2761 gcgtctggtg cacgttacac tgacggaatc tatgttttct ggtcgaaagg cgatgaagcg
     2821 actgtctata aacgcgaccg catcgtattg aataactgtc agttacaaaa tccacagcgt
     2881 tgagattttt ccaggggcgg cgcacaatag cgtcacccac tgacaatccg taaagaaaac
     2941 catgtctgat aacgacgaat tgcagcaaat cgcgcatctg cgccgtgaat acaccaaagg
     3001 cgggttacgc cgtcgcgatc ttcccgccga tccattaacc ctttttgaac gttggctctc
     3061 tcaggcgtgt gaagccaaac tggcagaccc taccgcgatg gtggtcgcta ccgtggatga
     3121 acatgctcag ccttatcaac gcatcgtttt actcaaacat tacgacgaaa aaggcatggt
     3181 gttttacacc aacctcggta gccgtaaagc acatcaaatt gaaaataatc cgcgcgttag
     3241 cctgctgttc ccgtggcata cccttgaacg ccaggtgatg gtgatcggta aagcagaacg
     3301 actttcgact ctcgaagtga tgaaatattt tcatagccgc ccgcgtgata gccagattgg
     3361 tgcatgggtt tcgaagcagt ccagtcgcat ttctgcccgc ggtatccttg aaagtaaatt
     3421 cctggagctg aagcagaagt ttcaacaggg cgaagtgcca ttgccgagct tttggggcgg
     3481 ttttcgcgtc agccttgaac agattgagtt ctggcagggc ggtgagcatc gcctgcatga
     3541 ccgctttttg taccagcgag aaaatgatgc gtggaagatt gatcgtcttg caccctgaaa
     3601 agatgcaaaa atcttgcttt aatcgctggt actcctgatt ctggcacttt attctatgtc
     3661 tctttcgcat ctggcgaaaa gtcgtgtacc ggcaaaggtg cagtcgttat atacatggag
     3721 attttgatgg caagcagtaa cttgattaaa caattgcaag agcgggggct ggtagcccag
     3781 gtgacggacg aggaagcgtt agcagagcga ctggcgcaag gcccgatcgc gctctattgc
     3841 ggcttcgatc ctaccgctga cagcttgcat ttggggcatc ttgttccatt gttatgcctg
     3901 aaacgcttcc agcaggcggg ccacaagccg gttgcgctgg taggcggcgc gacgggtctg
     3961 attggcgacc cgagcttcaa agctgccgag cgtaagctga acaccgaaga aactgttcag
     4021 gagtgggtgg acaaaatccg taagcaggtt gccccgttcc tcgatttcga ctgtggagaa
     4081 aactctgcta tcgcggcgaa caactatgac tggttcggca atatgaatgt gctgaccttc
     4141 ctgcgcgata ttggcaaaca cttctccgtt aaccagatga tcaacaaaga agcggttaag
     4201 cagcgtctca accgtgaaga tcaggggatt tcgttcactg agttttccta caacctgttg
     4261 cagggttatg acttcgcctg tctgaacaaa cagtacggtg tggtgctgca aattggtggt
     4321 tctgaccagt ggggtaacat cacttctggt atcgacctga cccgtcgtct gcatcagaat
     4381 caggtgtttg gcctgaccgt tccgctgatc actaaagcag atggcaccaa atttggtaaa
     4441 actgaaggcg gcgcagtctg gttggatccg aagaaaacca gcccgtacaa attctaccag
     4501 ttctggatca acactgcgga tgccgacgtt taccgcttcc tgaagttctt cacctttatg
     4561 agcattgaag agatcaacgc cctggaagaa gaagataaaa acagcggtaa agcaccgcgc
     4621 gcccagtatg tactggcgga gcaggtgact cgtctggttc acggtgaaga aggtttacag
     4681 gcggcaaaac gtattaccga atgcctgttc agcggttctt tgagtgcgct gagtgaagcg
     4741 gacttcgaac agctggcgca ggacggcgta ccgatggttg agatggaaaa gggcgcagac
     4801 ctgatgcagg cgctggtcga ttctgaactg caaccttccc gtggtcaggc gcgtaaaact
     4861 atcgcctcca atgccatcac cattaacggt gaaaaacagt ccgatcctga atacttcttt
     4921 aaagaagaag atcgtctgtt tggtcgtttt accttactgc gtcgcggtaa aaagaattac
     4981 tgtctgattt gctggaaata atgcattaag tggaaagggg gagtgagaaa tcactccccc
     5041 tggtttttat acagggaaca tgatgaaaaa tattctcgct atccagtctc acgttgttta
     5101 tggtcatgcg ggtaacagtg cggcagagtt tccgatgcgc cgtctgggcg cgaacgtctg
     5161 gccactgaac accgttcaat tttctaatca cacccaatac ggcaaatgga ctggctgcgt
     5221 gatgccgccc agccatttaa ccgaaattgt gcaaggcatt gccgccattg ataaattaca
     5281 cacctgtgat gccgtgttaa gtggctatct gggatcggcg gagcagggtg aacatatcct
     5341 cggtatcgtc cgtcaggtga aagccgcgaa tccgcaggcg aaatattttt gcgatccggt
     5401 aatgggtcat ccggaaaaag gctgtatcgt tgcaccgggt gtcgcagagt ttcatgtgcg
     5461 gcacggtttg cctgccagcg atatcattgc gccgaatctg gttgagctgg aaatactctg
     5521 tgagcatgcg gtaaataacg tcgaagaagc ggttctggca gcgcgcgaac tcattgcgca
     5581 agggccacaa attgtgttgg ttaaacacct ggcgcgagct ggctacagcc gtgaccgttt
     5641 tgaaatgctg ctggtcaccg ccgatgaagc ctggcatatc agccgtccgc tggtggattt
     5701 tggtatgcgc cagccggtag gtgttggtga tgtgacgagc ggtttactgc tggtgaaact
     5761 gcttcagggg gcaacgctgc aggaggcgct ggaacatgtg accgctgcag tctacgaaat
     5821 catggtgacc accaaagcaa tgcaggaata tgagctgcaa gtggtggctg ctcaggatcg
     5881 tattgccaaa ccagaacatt acttcagcgc aacaaagctc tgaaatatct catttcggcc
     5941 cacaacgacc tgtgggccga tggatattac tttaagcctt ccgctgacag cgcgtcttgt
     6001 acttccggac gttcagccat acgttgcata aatgctgcaa tgtgctctaa cccttccaga
     6061 ttcagtttca ccgcgtatgc ccagcgcaga accgtaaaca gataggcatc agcaattgta
     6121 aatctttgcc cgcagatcca gtgctcatcc ttcagtgcct cgttcacata ttgcagcttc
     6181 ttctccagct gcgcgcgaac tgtcggtttg tactcttccg gtgtatcagg gcgaaacaga
     6241 ggtgtgaaac ctttatgcag ctcggtggcg atgtaattca gccattcgat ggttttatag
     6301 cgggaaatac tgtttaccgg tgccagcaac tggcggtcgg ggacgctgtc ggcaagatac
     6361 tgcataatcg ctacgccttc cgtcagcaaa gtaccgtcat ccagcagcaa tgcaggcacc
     6421 tgccccttag ggttaacggc aaagtaatcg tcaccgtttt cgagacgttt tttcattaaa
     6481 tccacactga cgagggtaaa atcctttccg ctctcacgca gggtgatatg ggaagcgaga
     6541 gagcaggcac ccggtttgta gaacaatttc atcggtaact ccttttgtct gtggtttaaa
     6601 gtatggtagt gcgttgctga acaaaaaaaa agccgctaac gaaaagttag cggcttgtaa
     6661 aagagtttcc ctgaatttta cgctacagca gctttcgccg ctttgtctgc agcgtcatcc
     6721 tgcgtcatac ggtgcagttt aggcgcggtc agcagcatca gtactgcaat aatggcagta
     6781 gcgacaccaa tctgcaagaa tacgcgacca taaacttcca gtgacatcag cgggtcggta
     6841 acgttatccg gcacagccat catacccgca acataaccac caatcaggtt tgcaccggca
     6901 gtggtcagga accagctacc cataatgaag cccatcagac gctgcggaac gagttgtgca
     6961 accattgcca gacccagacc agagatcatc agttccccga tgctctgcag gccatagctt
     7021 gcgaccagcc agcttacaga cacgatacca gcgtcagacg cgaatttcgc acccagcggc
     7081 agaatcagga acgcaccaga acacatcacc atgccgattg caaacttggt tggcatcggc
     7141 agggtatcgc ccatcttgtt atagatagcg gccagaatcg gactaccgat gatgatccag
     7201 aacgggttca gtgcctgata ctgctcaggt tctacggcca gacccagaat ggagtgctca
     7261 acgttacgaa tcgcaaagaa gttcagtgac gttggcatct ggctgtacag cacgaagaag
     7321 ataatggctt cgagcatcag gatgaaggca acgatcattt tacgacgcgc agcacctttc
     7381 atcgcgaagg cttctttacc gaagataacc acgataccga aggcaacaac gcccagcgcc
     7441 atacgcgcaa cttcctgatt gtgcagcagc caggtggcga tagcgatcag tgccacaaca
     7501 ccaataatgg tcagcagcag gttacggtag ttgataggct cgaagtctgg ttttgaaccg
     7561 tactgtttaa cccagcgttg gcagaaggcg aagttaacga tagtgatcag caggcctaca
     7621 acgctcaacg caaacgcaac actccagccg tatttcgcgg ccagccacgg cgtagcaatc
     7681 atagagaaga aagagccgat gttgacggac atgtagtaca tggtgaatgc accgtccaga
     7741 cgcgggtcgt ttttctcata gcatgtagaa agcagagaag acgggttagc tttaaacagg
     7801 ccgttaccga ccgcaatagc cgccataccc atataaacga taccggcgtc gtgaccagac
     7861 caggcaacca gagcataacc aatcgccagc acaatagcgc cgagcataat tacgcgttta
     7921 gtacccagta ccttgtcacc taaccagccg ccgatagcga ccagaccata aaccagggca
     7981 ctaaaggaag agaaaagggt gattgaatcc gcttcagaca tacccagttg tttaaccagg
     8041 taaacagcca taattccttg taggccgtaa taaccaaaac gttcccataa ctcaatcgag
     8101 aagatgagat agaacgcctt cggttgtttg aaagcgttca aactgacgct ttcagttggt
     8161 ttttggtttg cagtggacac ttttacctct ttttttacat cccatattaa cgggggtgct
     8221 ttctcgcgtt atgtctaaaa aacatacgcg caattgttat agtgggaggg gaaacggcag
     8281 gtaatgttca ctatcttgca ctttcagaca agtcctttgt aataatctgt tacatataac
     8341 tgaggcgggg aattcaacca ttccgggaaa tgttatccag cgttaaattt ttcaaaaaac
     8401 gtaacggcag attttttcac tgcactgaga ggggggtgat ggcttaacgg cgatatgttc
     8461 tgctatttag cgtttttact agtgagatag tccagtttct gaaaaatagc cagtgtaatg
     8521 ttttgtaggt caatatttgt tgcgtgtgta aatttaaatg attttaacct gattcttgca
     8581 atgtttttac attgcgacag catcgtgatc ttgatcacga atgaatagaa aatttcgcta
     8641 ttaaaaaaac aattaacctg catttttggt tatccgcaaa cggattacct gatgataacg
     8701 ctaagcaatg gcatcattga cgttaaaaag atgttttaaa agaggataaa gaaaggttat
     8761 caatggggta atcggtgtta ccccttttct tcagatgtca actttctctt tgtattcaca
     8821 aagatcttca ataatacaag agccacagcg gggcttgcgg gcaatgcagg tataacgccc
     8881 gtgcaggatc aaccaatggt ggcagtcgac tttaaactct gccggaacca ctttcagtag
     8941 cttttcttct acctgttcga cgtttttccc cggcgcaaat tgagtacgat tacaaacgcg
     9001 gaaaatgtgc gtgtcgacag caatagtcgg ccagccgaat gcagtgttta atacgacgtt
     9061 ggctgtttta cgacctacgc cgggcagggc ttcaagcgca gcacgatctt ccggaacctc
     9121 gccattatgc tgctccagta agatacggca ggttttgatg atattttctg ctttgctgtt
     9181 ataaagccca atcgttttga tataggtttt caccccttca acgcccagtt caagcatcgc
     9241 tgcaggcgta ttcgccaccg ggtagagttt cgccgtcgcc ttattaacac tgacatcggt
     9301 cgcctgagcg gaaagcagta cggcaatcag caattcaaaa ggcgaactga aattaagctc
     9361 ggtggtggga tgaggattgt tctcacgcag gcgagtgagg atctccaggc gttttgcttt
     9421 gttcatcaga cattccctgt ttcaccgttt ggcaatgcac gttctgcagc tgcttcagca
     9481 cggcgctttt tcattctttc atcaatcagg tattttcctg ccagcatcag tcccaggcca
     9541 ataaatgcac ctggaggcag catcgccagc aggaaagggg agtcggtgtg gaaaatttcc
     9601 acgcgtaata cttttgccca gctacctaac agcgcatctg caccgtcaaa caatgtgcca
     9661 ttgccgataa tttcgcgtag tgaacccagc acgaacatgg cgcaggttgc gcccatacca
     9721 attgaaaagc cgtccagtgc cgaaagcgcc ggaccttttt tggcggcgaa ggcttcagcg
     9781 cggcccacaa cgatacagtt agtgacaatc agcggaataa aaatccctaa tgattgatac
     9841 aggccaaagg cgtaggcgtt gatcagcatc tgtacagcgc tgaccaccga ggcgatgatc
     9901 atcacgtaaa tgggaatgcg gatctcggct ggcgtccagt gacgcagcgt cgaaatggtc
     9961 aggttggtca gcgtcagtac cagcgtagtc gcaagtccta aacccagagc gttagtggca
    10021 gtggacgtga ccgccaacag aggacaaagg ccgagcaact ggaccagcgc agagttgttt
    10081 ttccacaacc cctgaacaat aacgtcttta atttcgctca cgctttattc tccacaggca
    10141 ggaagttgag aaagttgtgc cggtaacgtc tgagcgtaca atccggcgcg ttttaccgca
    10201 ttaaccaccg cgcggggagt aatcgtcgcg ccggtgaact ggtcgaaatc accaccatct
    10261 ttcttcaccg cccagtgcgc atcatctgca ccactgattt ttttacccgc aaaatgggtg
    10321 atccagtcag aaaggcgcag ttcgatttta tcgccaagcc ctggcgtttc gtggtgctct
    10381 gtcacgcgcg tgccaagtac cgtgccgtta aaatcggctc ccaccagcag ctgaatcgca
    10441 ccggaatagc catctggcgc ggttgcttcc agaacggcgg ctaccggttt gtcatcctgt
    10501 ttggcgatgt aaacccgatg ctcaccttta cctaactctg gcgcagttac cagatagcaa
    10561 ctctgtgcca gcgcattgtt atagcgttcg gctggcagca cctgatcaaa taatgccttt
    10621 tgttgcagac tggcctgttc agcaatcgtc gttttggtca tctggttgat ggccgcagtt
    10681 aaccctgttg aacccgctgc aaatagcgct aacgtaatgc cgtgttttcg gatagttttc
    10741 agcatggttt accctttgcg atggccgtag acgcgcggac gcgtgtagta atcgatcaga
    10801 ggaaccgtga tgttcgccag caggacggca aaagccacgc cgtcaggata gccgccgaaa
    10861 ctgcggatca accagactaa taagcccgca agcgcgccga aaataagacg accacgattg
    10921 gtcgtagaag cggtaaccgg gtcagtcaaa ataaagaatg cgccgagcat ggtcgcacca
    10981 gacagcagat gaatttgcgg tgctgccagt gtttctggtg agaacaacca gcccaacgtt
    11041 gcgcataacg ccagcgttac taagaagctg aggggaatat gccagcgaat cgctttctgc
    11101 catagcaacc acaggccgcc agccagccag gcgagattta cccattgcca accagcgccc
    11161 gccagaatac cgctgtagat cggatattgc ataatctgtt caaccgaatg accggcacgg
    11221 acagaggttt taaatgtatc cagcggtgtc gcctgactaa tgccatcaat acctaagcgt
    11281 agtgtgttca tatcaccacc actggcggta tgcccgctga aaataacctg tatggcgtcg
    11341 ataaaaccag ggatgttgac cgcaatttca tgtggcggta accagctggt catctgcacc
    11401 gggaaggaga tcagtaagac cacataacca atcattgctg gattaaacgg gttttgcccc
    11461 agaccgccat acaactgttt agcgatgatc accgcaaaca ccgtacccag cacgaccatc
    11521 caccatggcg cgaggggggg aatacttacc gccagcaata agcctgtcag caatgctgag
    11581 ttatctttca acgttgcggc taccgactgc ttgcgtagtt tgagtacgag agcttcggct
    11641 aacagagcac taaccgatgc caacaggatc tgaacgagag taccccaacc aaaaaaccac
    11701 agttgcgctg cgattcctgg cacggctgcg agcaacacca acagcataat gcgcgatgtc
    11761 tggcgctggt tatggatata aggggagcta gctattctga ataccattta gtcctcgttt
    11821 acaaccttct gctgggcggc ttttttggcc tgagcgcggg caatagccgc ggcaactgcc
    11881 gctttgcgcg gatcaacctg ttcttctggt tctgcgttag tctgttgctg ttccagcttg
    11941 cgcgctttgg cacgggcaat agcggcttcg acggcggctt tgcgcggatc aacctgttct
    12001 tctggttccg cattagcctg ttgctgttcc agtttgcgcg ctttggctcg ggcaatagcg
    12061 gcttcgacgg cggctttgcg cggatcaacc tgttcttctg gttccg
//
LOCUS       DAPHDW010000133        12093 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.135, whole genome shotgun sequence.
ACCESSION   DAPHDW010000133 DAPHDW010000000
VERSION     DAPHDW010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 12093)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12093)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12093
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.135"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..748
                     /locus_tag="RH057_003588"
     CDS             32..748
                     /locus_tag="RH057_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3129905.1"
                     /translation="MVAKINLLSRLTPLLFVFAPFLAQSNMTVYPMAVSINSQGEGNV
                     RVISKSNEVQYIKATVFRIDNPSMPQENEVEIKSGDANHLVVMPPKFALPAGSSKTVR
                     FVAMEPEQKEKNYRVKFEAVPSIDDVATDKKDLSMQLTVNLIWGIVVSVPPQQPIAKL
                     EVNAAQKLVNAGNQRLKILTIAYCKNNSKENCKIQTVNKNIFPGQERNLESISGYDKI
                     VVKYNNWITKDNGEFELAVH"
     gene            778..1278
                     /locus_tag="RH057_003589"
     CDS             778..1278
                     /locus_tag="RH057_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3129906.1"
                     /translation="MKKVFAKSLLVAAMFSVAGSALAVQKDITVTANVDAALDMTQTD
                     NTALPKAVEMQYLPGQGLQSYQLMTKIWSNDVTKDVKMQLVSPAQLVQSLDASKIVPL
                     TVTWGGEEIKADAATTFTATKIFASDALTNGSLAKNLMFAQTTKGVLETGIYRGVVSI
                     YLSQDI"
     gene            1370..4054
                     /locus_tag="RH057_003590"
     CDS             1370..4054
                     /locus_tag="RH057_003590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HDV3129907.1"
                     /translation="MDKKLLALLILASLSPAEATLTKIPAGFEVIAQGQQEYIEVYFS
                     GKSLGKYYAMVNLDTVTFLDPASLYNKLELDVDDQKIAHIVKEKLSQPLARHGELACG
                     YVRTDSGCGFLNTDTLEIIYNDEESSATLFINPQWNSAFDAKSLYLNPDKNTVNAFIH
                     QQDINVLAQDDYQSLSIQGNGALGITENSYIGAHWNFNGYDADDVSDSNADVSDLYYR
                     YDFLRRYYVQAGRMDNRTLFNAQGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGT
                     PVMVLLSRNSRVDAYRNEQLLGSFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRT
                     ELVPFTKTGGLTDGNAQWFLQAGKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANA
                     DDVSAFELGNNWTADLGGVGNLAISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTN
                     SDGDACTTDSRESYNALSCYESISATVSQNFVGWNMMLGYTRTQNNTDDSLRWDKQQS
                     FENNYLRQTTAQSISETVQLSASRAFVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSF
                     SLSDTPTMDSNNNSHSTNVSTDYRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGL
                     NTDTINSAVNGRWDGQYGNVYATVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGA
                     SGTDDLLGAVLVDVKGFSEQDEESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGF
                     VEVNDSSQGNQQGTTNIINGAGNRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKK
                     FPIVGLNSAMLLVAEDGGFTLEINGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDV
                     TCSVVTYSQLPESIQVQAQLKQPKLRGNVQTAQREVAP"
     gene            4051..5139
                     /locus_tag="RH057_003591"
     CDS             4051..5139
                     /locus_tag="RH057_003591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HDV3129908.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPPGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(5227..6525)
                     /gene="exuT"
                     /locus_tag="RH057_003592"
     CDS             complement(5227..6525)
                     /gene="exuT"
                     /locus_tag="RH057_003592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HDV3129909.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     IARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            7008..8420
                     /gene="uxaC"
                     /locus_tag="RH057_003593"
     CDS             7008..8420
                     /gene="uxaC"
                     /locus_tag="RH057_003593"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HDV3129910.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGEPLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            8435..9922
                     /gene="uxaA"
                     /locus_tag="RH057_003594"
     CDS             8435..9922
                     /gene="uxaA"
                     /locus_tag="RH057_003594"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HDV3129911.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQPDFQDLPAQ
                     AADREVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVVFANGKQTCNERNDFRELAIFKSGVTL"
     gene            10005..10556
                     /locus_tag="RH057_003595"
     CDS             10005..10556
                     /locus_tag="RH057_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HDV3129912.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEVSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAVDERG"
     gene            complement(10561..11805)
                     /gene="sstT"
                     /locus_tag="RH057_003596"
     CDS             complement(10561..11805)
                     /gene="sstT"
                     /locus_tag="RH057_003596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HDV3129913.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPIGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
BASE COUNT         3254 a         2876 c         3078 g         2885 t
ORIGIN      
        1 tttggaaaca atattgtaag atcaaattta tatggtggcg aaaattaatc tcttatcacg
       61 gcttacgccg cttttattcg tgttcgctcc ttttttggca cagtcaaata tgactgttta
      121 tcctatggct gtttccatta atagtcaggg tgaaggcaac gttcgcgtaa tatctaaaag
      181 taatgaagtt cagtacatca aggcgacagt attccgtatc gataatccat cgatgcctca
      241 ggaaaatgaa gttgagatta aatccggtga tgcaaatcat ctggttgtta tgccacctaa
      301 attcgcttta ccagccggta gtagcaaaac cgtacgtttt gttgcgatgg aaccagagca
      361 aaaagagaaa aattatcgcg ttaaatttga agcggttccc agtattgatg acgttgccac
      421 agataaaaaa gatctctcta tgcagttaac agttaactta atttggggga ttgttgttag
      481 tgttccacct cagcaaccta ttgctaagtt agaagtaaat gctgcccaaa aattagttaa
      541 tgcagggaat caacgcttaa agattttaac gatcgcttat tgtaaaaata atagcaaaga
      601 aaattgtaag atacagaccg taaataaaaa catcttccct ggtcaggaaa gaaatcttga
      661 aagcatatct ggctacgaca aaatcgttgt caaatataat aactggatca ccaaagataa
      721 tggcgagttt gaactggcgg tccattaatt atcattgtaa taaacgaagg aatagtaatg
      781 aaaaaggtat ttgcaaaatc tcttctggtc gcagcgatgt tttctgttgc aggctccgca
      841 ttggcagtac aaaaggatat tactgtaacg gccaacgttg atgccgctct ggatatgaca
      901 cagaccgata acaccgcgtt gccgaaagca gtagaaatgc aatatctgcc ggggcagggt
      961 ctgcaatctt accagttgat gaccaaaatc tggtctaacg acgttaccaa agatgtaaaa
     1021 atgcagttag tttccccggc gcaactggtt caaagcctgg atgctagcaa gattgttccg
     1081 ctgacagtaa cctggggcgg tgaagaaatt aaagctgatg cggcaaccac ttttaccgca
     1141 accaaaatct ttgcttccga tgcgttaact aacggttctc tggctaaaaa cctgatgttt
     1201 gctcaaacca ccaaaggtgt tctggagaca ggcatctacc gtggtgtagt aagcatttat
     1261 ctgtctcagg atatctaatc cagataacag aattaacacg taacaaaggg aggggtagcc
     1321 ctcccttatt ttaactgttt tagtatttgt tatggatttc aggattgcca tggataaaaa
     1381 attactggct cttttgatcc tggcgagtct cagcccggca gaggcgacat taaccaaaat
     1441 tcccgcaggg tttgaggtta ttgctcaggg acagcaggag tatatcgagg tttatttttc
     1501 agggaaaagt ctcggtaaat attatgcaat ggttaatctt gataccgtaa catttcttga
     1561 tccagcaagt ctatataaca agctggaact ggatgtagac gatcagaaaa tcgcgcatat
     1621 agtgaaagaa aaattatcgc agccgctagc tcgccacggt gaattggctt gcggttatgt
     1681 acgtactgac tcagggtgtg gttttctgaa taccgatacg ctggaaataa tctataatga
     1741 tgaagaaagt tcggcaacgt tgtttattaa tccgcaatgg aattcagctt tcgatgcgaa
     1801 gtcattatat ttaaatccag acaaaaatac tgttaatgct tttatacatc agcaagacat
     1861 caatgttctg gcacaggatg attaccaatc gttgtctatt cagggaaacg gtgcgctggg
     1921 aataacagaa aatagttata ttggtgcaca ctggaatttc aacggttatg atgcagatga
     1981 tgtcagtgac agtaatgctg acgtcagcga tctctattat cgttatgatt ttttacgtcg
     2041 ttattatgtg caggcgggtc gcatggacaa ccgcacacta tttaatgcac aaggcgggaa
     2101 ctttaccttt aactttctgc cactcggtgc aattgatggg atgcgtatcg ggtcgacact
     2161 gagctattta aaccaggcgc aaagccagca aggaacccca gtaatggttc tgctttcgcg
     2221 caattctcgt gttgacgctt atcgtaatga gcaacttttg ggatcgtttt atctcaatag
     2281 tggttcgcaa tttattgata ccagttcctt tccgcctggc agctacagcg tggcattaaa
     2341 agtctacgaa aataaccaac tcacccgcac cgagcttgtg ccgtttacca aaaccggcgg
     2401 tctgactgac ggaaatgcgc aatggttctt acaggcaggt aaaactacat cacaggtttc
     2461 tgatgatgaa agttcagctt atcagctagg agtacgcctg ccattacatc cgcaatatga
     2521 gctctacgca gggctggcga atgccgatga tgtgagtgct ttcgagttag gtaataactg
     2581 gacggcagat ttaggcgggg tggggaatct tgcaatcagc gccagcgtgt tccgtaacga
     2641 tgacggcggc aaaggtgata tgcaacaggc caactggagt aatccgggat ggccgacgtt
     2701 gggcttttat cggaccaact ctgacggcga tgcttgtaca accgacagca gagagagcta
     2761 taacgcctta agctgttatg aaagtatttc cgcgacggtt tcacagaatt ttgtcggctg
     2821 gaatatgatg ctgggttata cccgcacaca aaataacact gatgatagtt tgcgttggga
     2881 taaacagcag agctttgaaa ataactatct tcgccagaca acagcgcaaa gtatctctga
     2941 aactgtacaa cttagcgctt cccgcgcttt tgtgatgcgt gactggattt tgagtacgtc
     3001 agtcggtgtt ttccatcgta atgacaacgg tggcgataac gacgacaacg gcttgtactt
     3061 atcgttttcg ttatctgaca cgccaacgat ggatagcaat aacaacagcc attcaacaaa
     3121 tgtgtctacg gattatcgtt atagcgaaca ggatggcgat caaacgtcat ggcagttatc
     3181 gcataccttt tataacgatt cattcagcca taaagaactt ggcgtaaccg tcggaggcct
     3241 gaacaccgat accataaaca gcgcggttaa cgggcgttgg gatgggcaat acggaaatgt
     3301 ctacgctacc gtatctgata gttatgaccg taagaatcat gatcatcttt cggcctttac
     3361 gggcacttat agctctacgc tggctgtcag tcgctatggc gttaatttgg gagccagtgg
     3421 tacagacgat ttgctgggtg cggtactggt ggatgtgaaa ggcttctctg aacaggatga
     3481 agagagtcag gatctgcaac tcgaagcgcg ggtggcaggc agccgaactt tgcagcttgg
     3541 tcaaagtgac agtgtgttgt tcccttatcc tggatttcag tctggttttg ttgaggttaa
     3601 cgacagtagc cagggcaatc agcaggggac aacaaacatc attaacggtg cagggaatcg
     3661 tgaattaatg ttgttgcctg gcaagctgcg ctatcgcgaa gtgtctgcca gctttaacta
     3721 caactatatc ggtcgtttat tactgccggc agcggtgaaa aaattcccga ttgtggggct
     3781 gaacagcgcc atgttactgg tagctgaaga tggcggattt acacttgaaa ttaacggcag
     3841 cgaaaaagag ctctatctgc tttccgggca gcaattcctt aagtgtccac taagtgttgt
     3901 aaagaaacgc gccagcattc gttacagcgg agatgttact tgtagtgtgg tgacttattc
     3961 acaattaccg gagtcgattc aggttcaggc acagttgaaa cagcctaaat tacgtggaaa
     4021 cgttcagacg gcgcaaaggg aggttgcacc atgagaaatc gattgattgc ggcgatattg
     4081 ggcttgtttg gcacgctcac tggcgttcag gcagctcctg acgtgaccag tgaaattacg
     4141 tatgatttgg catctggtag agcggattat tacttctgga aggatgaagc gtcggcagga
     4201 aataatggat atatgtggta tgaatgttcg tatcctgacc tccaacaaac ctgtacagct
     4261 aatggaaata tatcgacagt acaaatctat ttaactgaac aacgcagtgg gatgcgttgg
     4321 ccggtaaaac tcaaaggatt taaaacagcc attgtaagta gcgatgaagc gccgccagga
     4381 tgcaaggggg gcaaagggct tcagacgaat cttaaggatt ctaatagatc ttcatgtaca
     4441 gaagatggtc aacattatta tatatacgat acaaagtttc ttacgctcta ccttgagcag
     4501 acagagatga agaatttgcc gattggtggc gtctggaagg ggaaagttaa attacattcg
     4561 aacagcccgg cccaggacta tttcgcaaat attaccctga atacgctcga ccccaaccat
     4621 attgacgtgt tcttcccgga gttcgcccac gccacgccaa gggtgcagtt agacttgcat
     4681 ccaacaggaa gcgttaacgg cagcaactac gcgcaagatc tgaccatgtt ggacatgtgc
     4741 ctgtacgatg gttttaacgg taatgccatc agttatgaaa tcatgctcaa agatgaaggg
     4801 cgaccagctg cagggcgcag agacggttac ttctctatct atcgtcaggg agggaccacc
     4861 accgacgagg gagaacgcat tgattaccgg gtcaaaatgt acaacccgga aaccggtggg
     4921 caaattgatg tgcgcaataa tgaaaatatg gtctggaaca gcattaacct gaaacgtgtg
     4981 cgtccggtgg tactgcccgg tattcgctat gccgtgatgt gtgtgccgac acccttgact
     5041 ctggcagtcg ataaattcag cgtgatggat aaacaggccg ggtactacat gggcaaattg
     5101 tcagtaatct ttacgccttc cttgccaacc atcaattaac cctgcaaacc ggacaaacgc
     5161 ttttttctat ataaacgacg aagccgtctg ataaggcggc ttcgtcatta caacttaagt
     5221 taaacgttaa tgttgcggtg cgggatcgtt atgtgtttcc tgcgccacct caatcgccgg
     5281 tttgttctgc aacacggtcc agataaccag cgcacctaac aggtcaaata ctgccagaac
     5341 tgcgaacagc gggctgaagc cgatggtgtc agccagtgca ccgacaacca gcgcaaacag
     5401 cgtacttgcc agccatgcgg acatcccggt taaaccgttc gccgtcgcca cttcgttacg
     5461 accgaacacg tcagaagaga gcgtaatcag cgcgccagac agtgcctggt gggcaaaacc
     5521 accgatacac agcagcataa ttgcgacata cgggttggtg aacagaccga tcatacccgg
     5581 gccaatcatc agcacagcac ccagcgttac gaccatctta cgggaaacga tcaggttcac
     5641 accaaaccaa cgctggaaca gcggcggcag gtaaccaccg aggatacaac cgaggtcagc
     5701 aaacagcatc ggcatccagg cgaacatcgc gatctctttc aggttaaagc cgtaaacttt
     5761 aaacatgaac agcgggatcc acgcgttaaa agtaccccag gccggttctg ccaggaaacg
     5821 cggcagcgcg ataccccaga actgacggtt acgcaggatc tgaccaacgg acattttctt
     5881 cgccgtgctc acctggtgct gggcttcctg accattaata atatagtcgc gttcttcatc
     5941 ggtcagatgc ttctggtcgc gtggatgttt atagaaaatc agccatgcca tcgcccagat
     6001 aaagctcaat gcaccggaga tgataaatgc catctgccag ctgtgcatta cgattgccca
     6061 taccaccagc ggcggcgcaa tcatcgcacc aatcgaagaa cctacgttaa agtaacctac
     6121 cgcgatggaa cgctctttcg ccgggaacca ttcggagctg gctttcagac ccgccgggat
     6181 catcgctgct tccgcggcac cgactgcacc acgagcaatc gccaggccac cccagctacc
     6241 agccagcgcg gttgcaccac agaacacggc ccacagcaca gcaaacattg cataaccgat
     6301 tttcgtaccc agcacatcca gtacatagcc cgctaccggt tgcatgaccg tataagcagc
     6361 agaataggct gcgatgatat aggaatactg ttgggtggag atgtttaact cttccatcag
     6421 agttggcgca gctgccgcca cagtgttacg cgtcaggtaa ccaagcacgg tgccaagcgt
     6481 caccagtgcg atcatatacc aacgtaaccc tttaatttta cgcatgtaaa acctcatcgt
     6541 tttgttatat ccgcaggaag cgcggccatc atcccgccag aggctgaaga tgttagttac
     6601 gaccgggtaa ccgaaaaaaa accggcacgc cccgaacgtt gccatgaaag tttcgtgcta
     6661 attcggcttc cgtaccggtt aacccgattc atctaacaaa tgaatgaatc ggcaatttgc
     6721 attccgtcga cttatgattt gcgacggcag aaagataact tgtcatacaa ctttaaaagg
     6781 tgagagccat cacaaatgtg cgaatatttg tagggacatt acctgacgac agcgaggcca
     6841 gtactggcgc ggcctgcaga gagatttacc actttgagag taattttttt aactacgttt
     6901 attgatctaa ctcacgaaaa tatcttcgga ctctggaaat tggtgtgata actttgtcag
     6961 catcgcacca taagcaagct agctcactcg ttgagaggaa gacgaaaatg actccgttta
     7021 tgactgaaga tttcctgtta gataccgaat ttgcccgccg tctgtatcac gactacgcaa
     7081 aagaccagcc gattttcgat taccattgcc atttgccgcc gcagcagatt gcggaagact
     7141 atcgttttaa aaacctgtat gacatctggc tgaaaggcga tcactacaaa tggcgcgcta
     7201 tgcgtaccaa cggtgtggcc gagcgtctgt gtaccggtga tgcgtctgac cgtgaaaaat
     7261 ttgacgcctg ggcggcgact gttccgcaca ctatcggcaa cccgttatac cactggacgc
     7321 acctcgaact gcgtcgtccg tttggtatca ctggcaaatt gctttctccg tcaactgccg
     7381 atgaaatctg gaacgaatgt aacgaactgc tggcgcagga taacttctcc gcgcgcggca
     7441 tcatgcagca gatgaacgtg aaaatggtcg gcaccaccga tgacccgatc gattctctgg
     7501 agcatcacgc agagatcgcc aaagacggct ctttcaccat caaagtgctg ccgagctggc
     7561 gtccggacaa agccttcaac atcgaacagg cgacctttaa cgactacatg gcgaagctgg
     7621 gcgaagtttc cgataccgac attcgccgct ttgctgacct gcaaactgcc ttgaccaaac
     7681 gtctggatca cttcgccgct cacggctgta aagtgtctga ccatgcgctg gacgtggtga
     7741 tgtttgctga agcgaacgaa gcggaactgg acagcattct ggcgcgccgt ctggctggcg
     7801 aacccctgag cgagcacgaa gtggcacagt tcaaaactgc ggtactggtg ttccttggtg
     7861 ccgaatatgc acgtcgcggc tgggtacagc agtaccacat tggcgcgctg cgtaataaca
     7921 acctgcgtca gttcaaactg ctggggccgg atgtaggctt tgactccatc aacgaccgtc
     7981 cgatggcgga agagttgtct aagctgctga gcaagcagaa cgaagaaaac ctgctgccga
     8041 aaaccattct ctactgcctg aacccgcgcg ataacgaagt gctgggcacc atgatcggta
     8101 acttccaggg cgaaggtatg ccgggcaaaa tgcagttcgg ttccggctgg tggtttaacg
     8161 atcagaaaga cggtatggaa cgtcagatga cccaactggc gcagctcggt ctgctgagcc
     8221 gctttgtcgg tatgctgact gacagccgta gcttcctgtc atacacccgt cacgaatact
     8281 tccgccgcat tctgtgccag atgatcggtc gctgggtgga agcgggcgaa gcaccggcgg
     8341 acatcaacct gctgggcgag atggtgaaaa atatttgctt taacaatgcg cgtgactact
     8401 tcgccattga actgaactaa ggtctggggt tgatatgcaa tacatcaaga tccatgcgct
     8461 ggataacgtc gcggtcgctt tagcagattt ggctgaaggc acagaagtca gtgtcgataa
     8521 ccagactgtt acgctgcgcc aggatgttgc tcgtggacat aaatttgcgt taacggatat
     8581 cgcaaaaggg gccaacgtca tcaaatatgg cctgccgatt ggttatgcat tggcggatat
     8641 tgcggcgggg gaacacgtcc acgcccacaa tacgcgcacg aatctgagcg atctggatca
     8701 gtatcgctat caacctgatt ttcaggatct gcctgcgcaa gcggcagatc gtgaagtgca
     8761 gatctatcgt cgcgctaacg gcgatgtcgg ggtgcgtaat gagctgtgga tcctgccaac
     8821 cgtgggctgt gtcaacggca tcgcgcggca aatccagaac cgtttcctga aagagaccaa
     8881 caacgccgaa ggtaccgacg gcgtgttcct cttcagccac acctacggct gctcacaact
     8941 gggcgacgat cacatcaaca cccgcaccat gctgcaaaac atggtgcgcc acccgaacgc
     9001 gggcgcagtg ctggtgattg gtctgggctg tgaaaacaac caggttgccg cattccgtga
     9061 aacgttgggc gatatcgatc ctgaacgcgt tcatttcatg atctgccaac agcaggatga
     9121 tgagatcgaa gccgggatcg agcatttgca tcagctgtat aacgtgatgc gcaacgataa
     9181 acgcgagcca ggcaaactca gcgaactgaa gtttggtctg gagtgcggtg gttctgacgg
     9241 tctttctggt attactgcta acccgatgct ggggcgtttc tctgactacg tgattgctaa
     9301 cggcggtact accgtactga ccgaagtgcc ggagatgttt ggcgcagagc agttgctgat
     9361 ggaccattgc cgcgacgaag caacgtttga aaaactggtc accatggtca acgacttcaa
     9421 acagtacttt attgcccatg accagccgat ctatgaaaac ccatcgccgg ggaacaaagc
     9481 gggcggtatc accacgctgg aagataaatc ccttggctgt acccagaaag cgggttccag
     9541 cgtcgtggtt gacgtgctgc gttacggcga gcgtctgaaa acgccggggc tgaacttgtt
     9601 aagtgcgccg ggtaacgatg ccgtagcgac cagcgccctg gcgggtgcgg gctgccatat
     9661 ggtgctgttc agtactggtc gtggcacgcc atacggcggc tttgtaccga cggtgaaaat
     9721 cgctaccaac agtgaactgg cggcgaagaa aaaacactgg atcgactttg acgcgggtca
     9781 gctgatccac ggtaaagcga tgccgcagtt gctggaagaa tttatcgaca ccatcgttgt
     9841 gtttgccaac ggtaaacaaa cctgcaacga gcgtaacgac ttccgcgaac tggcgatctt
     9901 caaaagcggc gtaacgctat aaagtcgaaa tgagttgtta ataaaaaacg gcgtttcata
     9961 atgtgaagcg ccgtttgctt ttccatacct gataaggatt tatcatgacc gcgtattggc
    10021 tggcccaggg cgtgggtgtc atcgcctttc tgattggtat cacaacattt ttcaatcgtg
    10081 acgaacgtcg cttcaaaaag cagctttcgg tctatagcgc cgttattggc gtacattttt
    10141 ttcttctggg cacctatccc gctggtgcca gtgccatcct taatgccatt cgtacattga
    10201 ttaccttacg cacgcgcagc ttatgggtaa tggcgatttt tattgtgctg actggcggaa
    10261 ttggcctcgc gaagttccat catcctgtcg aactattgcc ggttatcggt acgattgtca
    10321 gtacctgggc gctgttccgc tgtaaagggc tcaccatgcg ctgcgtgatg tggttttcaa
    10381 cgtgttgctg ggtgattcac aacttctggg cggggtcgat aggcggcaca atgattgagg
    10441 ttagttttct gcttatgaat ggcttgaata tcattcgttt ctggcggatg cagaaaaggg
    10501 gaattgatcc gtttaaagta gagaaaaccc ctcccgccgt agacgaaagg ggttaaacaa
    10561 ttaattacgc agggcgctat ttgccagacg atcgtcttcc gcctggcaag ctgccgcagt
    10621 gaacagcacg tcagttgaag agttcagcgc ggtttcgcaa gagtcctgca atacgccgat
    10681 gataaagccg acggcaacca cctgcatggc gatatcgttc gaaataccga acatattaca
    10741 ggccagcggg atcagcagca gagaaccccc cgccacgccg gatgcgccac aggcacacag
    10801 agaagccacc acgctcaaca gcagcgccgt gggcagatcg accgggatac ccagcgtatt
    10861 aaccgcagcc agcgtcaaca cggtaatggt gattgctgcg ccagccatat tgatggtggc
    10921 acccagcgga atagaaacgg aataggtatc gcgatccaga ttcagctttt cacacagtgc
    10981 catattcacc ggaatgttcg ccgcagagct gcgggtgaag aaggcataca cgccgctttc
    11041 gcgcaggcac agcagcacca gcgggaacgg gttacgacga attttccacc acaccagcaa
    11101 tgggttaacc accagcgcca ccagtaacat acagccaacc agcacgacca gcagttgcgc
    11161 gtagccccac agtgtggaga aaccggtggt tgccagggta gaagaaacca gaccaaaaat
    11221 accaatcggt gcgaagcgaa tgaccagttt caccataaag gtgacggcat tcgacatatc
    11281 gttgaccagg tttttggtgg tctcgttacc gtgacgcagt gcgaaaccga ggccaattgc
    11341 ccacaccagg atcccgatgt agttaccttt cagcagcgcg tcgatggggt tggaaaccat
    11401 gctcattacc agcccgcgca tcacttcgac aatgcctgac ggcggcgaaa tatcacccgc
    11461 gctactggat aaatgcaggg tagaagggaa ggcaaagctg aagactactg cggccagagc
    11521 agcagagaag gtgcccagta gatagaggaa caaaataggg cggatattgg ttttctgccc
    11581 gtgctggtgg ttagcaatag atgccattac cagcatcaac accaggatgg gggcaacggc
    11641 tttcagtgcg ccgacgaaca aagtacctaa cagaccaaca gcttccgccg cgggttttga
    11701 gatccatgcc agaagaatcc ccagaacaag gccgaccagg atttgtttta ccaggctgcc
    11761 atgagccaga cgccggaata gccccggtga acgttgcgta gtcatttttc gatcctttca
    11821 ttgtgttgtc gcacatccct ggtgcgttct gtcgacgcat ctttcaggat gtaacaaagt
    11881 gtttgcaagg tcgagtataa ggaaagttac agcttcggga agcgaaaaat gctggatttt
    11941 aaagagaggt catattttgc aacttaattg ttacatttgt ttgacataaa cagagggaag
    12001 gactggctac taaggctgat tagataacat atgggcaaaa ctatttggaa tatgagaacg
    12061 gttgcgctcc tttaatgtgt tatgccatat aaa
//
LOCUS       DAPHDW010000134        11910 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.136, whole genome shotgun sequence.
ACCESSION   DAPHDW010000134 DAPHDW010000000
VERSION     DAPHDW010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.136"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..612
                     /locus_tag="RH057_003597"
     CDS             49..612
                     /locus_tag="RH057_003597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3129914.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     KAWLVGAADAPDLGNFEANTTFQITYL"
     gene            694..3336
                     /gene="yfcU"
                     /locus_tag="RH057_003598"
     CDS             694..3336
                     /gene="yfcU"
                     /locus_tag="RH057_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
                     /protein_id="HDV3129915.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDLKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDWD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKKWEWSRYYAWRALPSLKAKLALGEDYLRSDIFDGFNYVGG
                     SVSTDDQMLPPNLRGYAPDISGVAHTTAKVTVSQMGRVIYETQVPAGPFRIQDLGDSV
                     SGTLHIRIEEQNGQVQEYDISTASMPYLTRPGQVRYKIMMGRPQEWGHHVEGEFFSGA
                     EASWGIANGWSLYGGALGDENYQSAALGVGRDLSTFGAVAFDVTHSHTKLDKDTAYGK
                     GSLDGNSFRVSYSKDFDQLNSRVTFAGYRFSEENFMTMSEYLDASDSGMVRTGNDKEM
                     YTATYNQNFRDAGVSVYLNYTRHTYWDREEQTNYNIMLSHYFNMGSIRNVSISMTGYR
                     YEYDNQADKGMYISLSMPWGDNSTVSYNGNYGSGTDSSQVGYFSRVDDATHYQLNVGT
                     SDKHTSVDGYYSHDGSLAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMG
                     GTRLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEAT
                     QSVVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDD
                     GNVYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDI
                     KPVIQEQTQQVTPTNPPVSVSANQ"
     gene            3356..4108
                     /locus_tag="RH057_003599"
     CDS             3356..4108
                     /locus_tag="RH057_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3129916.1"
                     /translation="MSDLLCSAKLGATTLALLLSAASLSAQASVTPDRTRLIFNESDK
                     SISVTLRNNDPKLPYLAQSWIEDEKGNKISSPLTVLPPVQRIDSMMNGQVKVQGMPDI
                     NKLPADRESLFYFNVREIPPKTNKANTLQIALQTRIKLFWRPKALENVSMKNPWQYKV
                     TLTRNGQEFTVNNPTPYYVIFSNASTQKNGNPAAGFSPIVMSPKTSAPLNVKMGSVPV
                     LTYVNDYGARMPLFFTCNGNSCQVDEEQSRKG"
     gene            4083..4631
                     /locus_tag="RH057_003600"
     CDS             4083..4631
                     /locus_tag="RH057_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3129917.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINGVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            4628..5098
                     /locus_tag="RH057_003601"
     CDS             4628..5098
                     /locus_tag="RH057_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3129918.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVVLPEADFEAWATLQVDYQ"
     gene            5095..5619
                     /locus_tag="RH057_003602"
     CDS             5095..5619
                     /locus_tag="RH057_003602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3129919.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTSFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSAIVTATFEYF"
     gene            5606..6478
                     /locus_tag="RH057_003603"
     CDS             5606..6478
                     /locus_tag="RH057_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HDV3129920.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNEL"
     gene            complement(6600..7151)
                     /gene="smrB"
                     /locus_tag="RH057_003604"
     CDS             complement(6600..7151)
                     /gene="smrB"
                     /locus_tag="RH057_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HDV3129921.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            7317..8249
                     /gene="prmB"
                     /locus_tag="RH057_003605"
     CDS             7317..8249
                     /gene="prmB"
                     /locus_tag="RH057_003605"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HDV3129922.1"
                     /translation="MDKIFVDEAVNELQTIQDMLRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPDAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLLAAREHFAIYKD"
     gene            8284..9369
                     /gene="aroC"
                     /locus_tag="RH057_003606"
     CDS             8284..9369
                     /gene="aroC"
                     /locus_tag="RH057_003606"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HDV3129923.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSQVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     LGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            9373..10197
                     /gene="mepA"
                     /locus_tag="RH057_003607"
     CDS             9373..10197
                     /gene="mepA"
                     /locus_tag="RH057_003607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HDV3129924.1"
                     /translation="MNKTAIALLALLACSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDLRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVPTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPPGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            10197..11006
                     /locus_tag="RH057_003608"
     CDS             10197..11006
                     /locus_tag="RH057_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV3129925.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVTLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            11006..11554
                     /gene="epmC"
                     /locus_tag="RH057_003609"
     CDS             11006..11554
                     /gene="epmC"
                     /locus_tag="RH057_003609"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HDV3129926.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLANGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            11588..11866
                     /locus_tag="RH057_003610"
     CDS             11588..11866
                     /locus_tag="RH057_003610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HDV3129927.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQQ"
BASE COUNT         2955 a         2890 c         3102 g         2963 t
ORIGIN      
        1 gtattgtaat gtttatattt ttaattattt atagtttaag gacaaataat gagtaagttt
       61 gttaaaacag ctattgctgc ggcaatggtg atgggcgttt tcacctctac ggcaacaatc
      121 gctgcgggta acaacggtac tgcacgtttt tacggcacca ttgaagactc agtgtgttct
      181 atcgttccgg acgatcacaa actggaagtt gatatgggtg atatcggtgc cgaaaaactg
      241 aaaaataacg gcaccaccac gccgaaaagt ttccagattc gtctgcaaga ttgcgtattt
      301 gatactcagg aaacaatgac cactaccttt actggtaccg tttcttctgc aaatagcggc
      361 aattattaca ccattttcaa taccgatacc ggtgcggcat ttaacaatgt tagcctggcg
      421 atcggtgact ctctgggtac ctcttacaaa agcggcatgg gtattgacca gaaaatagtg
      481 aaagacactt ctaccaataa aggcaaagcg aagcagacac tgaactttaa agcctggctg
      541 gtcggcgcag ctgatgcgcc agatctgggt aatttcgaag caaataccac cttccagatt
      601 acttacctgt aaggcatacc cccatagcga cactgctatg ggggatttaa atggattttg
      661 accgaacaag aataaaaacg gatagaaacg tgtatgcctg accattctct ttttcgatta
      721 cggattcttc cgtggtgcat tgcgctggca atgtcaggga gttatagcag tgtctgggct
      781 gaagacgaca ttcagtttga ttcccgtttt ctggaattaa aaggcgacac aaaaattgat
      841 ctgaagcgtt tttccagcca gggatatgtt gagcccggaa aatacaattt acaggttcaa
      901 ctaaataaac agccattggc ggaagagtac gatatttact ggtatgctgg tgaagatgac
      961 gcgagcaaaa gctatgcttg tctgacaccg gaactggtag cgcagtttgg tttaaaagaa
     1021 gacgtggcga aaaatctgca atggagccac gatgctaaat gcctgaaatc cggtcaactg
     1081 gaaggcatgg aaattaaggc tgatttaagc cagtccgcat tagtcatttc actgccacag
     1141 gcttacctcg aatatactta tcccgactgg gatccgcctt cacgttggga tgacggcatc
     1201 tccgggatcg tcgcggacta cagcatcaac gcacaaaccc ggcacgaaga aaatggcggt
     1261 gatgatagta acgagatcag cggcaacggg acggtcgggg ttaacctggg gccgtggcgt
     1321 atgcgtgctg actggcagac taactatcaa catactcgca gtaatgatga cgatgaattc
     1381 agcggcgatg aaactcaaaa aaaatgggag tggagtcgct actatgcctg gcgggcgtta
     1441 ccatcattaa aagccaaact ggcgctgggc gaggattacc tcagatccga tatttttgat
     1501 ggttttaact atgttggtgg cagtgtcagt actgacgatc aaatgttgcc tcccaatctg
     1561 cgcggctacg cgccagacat ttccggcgtg gcacacacca cagcaaaagt gaccgtcagc
     1621 cagatggggc gtgtgattta cgaaacgcag gttccggctg gaccgtttcg tattcaggat
     1681 cttggtgatt ctgtctccgg tacgttgcat attcgcattg aagaacagaa cggccaggtg
     1741 caggaatatg acatcagcac cgcctcgatg ccatacctca ctcgcccagg tcaggttcgc
     1801 tataagatca tgatgggccg tccgcaagag tggggacacc atgtcgaggg tgaatttttt
     1861 tctggtgctg aagcttcctg ggggatcgct aacggctggt cgttatatgg cggcgcactg
     1921 ggagatgaaa actatcagtc tgcggcgctt ggcgtcggtc gcgatttgtc tacattcggc
     1981 gcggtcgcgt ttgatgttac tcactcgcac accaaactgg ataaagacac cgcttatggc
     2041 aaaggttcgc tggacggtaa ctccttccgt gtgagttatt ccaaagactt tgaccagctc
     2101 aacagccgcg ttacttttgc tggatatcgc ttctcggaag agaactttat gaccatgagc
     2161 gagtatctgg atgccagtga cagcggaatg gtacgcacgg gcaacgacaa agagatgtac
     2221 accgccactt ataaccagaa cttccgcgat gcgggtgttt cggtttatct caactatacc
     2281 cgccatacct actgggatcg cgaggagcag acaaactaca acatcatgct ctcgcactat
     2341 ttcaatatgg gcagtattcg taatgtcagc atctcgatga ctggctaccg ttacgagtat
     2401 gacaaccagg ccgacaaagg catgtacatt tcgctcagta tgccgtgggg cgacaacagt
     2461 accgttagct ataacggtaa ctatggcagt gggacggaca gcagtcaggt cggttatttc
     2521 agccgtgtcg atgacgcgac tcactatcag ttgaacgtcg gcaccagtga caaacacacc
     2581 agcgttgacg gctattacag ccatgatggt tcgctggcgc aggttgacct cagtgcgaac
     2641 taccatgaag ggcaatacac ctctgcgggc ttgtcgttac agggcggcgc aacgcttact
     2701 gcccacggtg gcgcacttca ccgtacccag aatatgggcg ggacacgctt gttgattgat
     2761 gccgatggcg ttgccgatgt tccggtagaa ggtaacggtg ctgctgttta taccaatatg
     2821 tttggtaaag ccgtcgtttc tgacgtcaat aactattacc gcaatcaggc gtatatcgac
     2881 ctcaacaaac tgccggaaaa cgccgaagca acccagtcgg tggtacaagc cacgctaact
     2941 gaaggggcga ttggctaccg caaatttgcc gtcatcagtg gtcaaaaagc gatggcggtg
     3001 ctgcgtttac aagacggcag ccatcctccg tttggcgcag aagtaaaaaa tgataacgag
     3061 cagacagtgg gccttgtcga tgatgacggc aatgtttatc tggctggggt gaaacctggc
     3121 gaacacatga gtgtgttctg gagtggtgtt gcgcattgcg atatcaacct gccggacccg
     3181 ctgcctgccg atctgtttaa cggcttgtta ctgccatgcc agcataaagg caacgtcgcg
     3241 ccggttgttc ctgatgacat aaagcctgtc attcaggagc agacgcaaca ggtgacaccc
     3301 accaatcccc ctgtttctgt ttcagctaac caataacgtg attaaggaat gatccatgtc
     3361 tgatttactt tgctctgcca aattgggtgc tacgacgcta gcgttactgc ttagcgctgc
     3421 cagtttgtct gcccaggctt ctgtaacgcc agatcgtacc cgtttgatat ttaatgaaag
     3481 cgacaaatca atcagcgtga cgttgcgcaa taacgatccg aaactgcctt atctggccca
     3541 aagctggatc gaagatgaaa agggtaacaa gatcagttca ccgttaactg ttctgccacc
     3601 ggtgcaacgc atcgattcaa tgatgaacgg gcaggtaaaa gtgcagggaa tgccggatat
     3661 caacaaattg cctgccgatc gtgaaagcct gttctacttc aacgtgcgtg agatcccgcc
     3721 aaaaacaaac aaagccaaca cgctgcaaat agcactacaa acgcgtatta agttgttctg
     3781 gcgaccgaag gcgctggaga acgtcagcat gaaaaacccc tggcaatata aggttacttt
     3841 aacccgcaac ggtcaggaat ttaccgtcaa taacccgaca ccgtattacg tcattttcag
     3901 taatgccagc acacagaaaa atggcaatcc ggcagccggt ttctcgccaa tagtgatgtc
     3961 gccgaaaacc tctgcaccgc tgaatgtgaa gatgggtagc gtgccggtac tgacctatgt
     4021 caacgactat ggcgctcgga tgccgctctt tttcacttgc aacggtaaca gttgccaggt
     4081 cgatgaagag cagagccgca aaggctgagg ccgggagagc cagtatgaaa ggatcttgtt
     4141 ctgtctgggc ggtaactggg ttactgatat gttgtgttgg tagcggcttt gcagttgcag
     4201 cgagtaagtc agtaagtatg aatttacggg tgcttgtcga tgcccctcct ccttgtacgg
     4261 taaatggtgc ggcagttgag tttggtaatg tattcattaa taagatcaat ggggttgatt
     4321 ataaacgtcc cattgactac tcgcttgttt gtaacaatct ggcaatggat gacctgcgtc
     4381 tacagatgca ggcgacaacg gtcgtgatta acggtgaaac agttatcagc acggggattc
     4441 ctgggtttgg tattcgggtt cagaaaagta gtgatcacac aatactcgac ttaacctctg
     4501 gctcatggtt gccatttaat ttttcctcgg gagttcccgt tctggaagct gtgccggtga
     4561 aacagagtgg tacaacgtta gcggcagctg aatttaacgc cagtgccaca attgtggtgg
     4621 attaccaatg aaacggatct cgcttatttt gctgtggggc ttttgctcga tggcactctc
     4681 aaacgtctct tttcacggtt atttagtgca gccacctaac tgcacgatta gcaacgctca
     4741 aaccattgaa ataacttttc aggatgtgct tattgatgac attaatggca gcaattatga
     4801 gcaaaccgtg ccgtattcca ttacctgcga tacggcggtt cgcgacccat taatggaaat
     4861 gaccttgagc tggagtggca caccaagcga ttttgataat gctgcagtaa gcagcaatat
     4921 cactggttta ggcattcagc tcaaacaggc agggcagagt tttacgatca atacgccact
     4981 tgtggtgaat gaaaccgatt taccggtgtt aacggctgtg ccagtaaaaa agagtggtgt
     5041 cgttttgcca gaggcggact ttgaggcctg ggccacgtta caggtagatt accaatgaag
     5101 aaaaagcgta cgttgttttt tattagtagc ctgatgttgc tgggcagtgg aaccacaata
     5161 gcaggagata atttgcactt taccggcaat ttaatcagta aatcgtgtac gccagtgatt
     5221 aatggtagcc agttggcaga agtacatttt cctgccattg ccgccagcga tttaatgaac
     5281 cttgggcagt cagagcgagt gccattggtg tttcaattga aagattgtca tagttctaca
     5341 tcgtttaatg tcaaagtaac gttgacagga acagaagata gtgctttacc cggctttctg
     5401 gcctttgact cctcgtcatc agcatccggc gctgggattg gtattgaaac tgcggcaggc
     5461 acatcggtgc caatcaacaa tacaacaggc gtcacgctcc cactgaatca gggaaataac
     5521 agcctaaatt ttaatacctg gttacaggca aagagcgggc gagatgtaac gtccggagac
     5581 tttagtgcca tagtgacggc aacctttgag tatttctgaa atgaaaaaat ggaccataat
     5641 tttcgcgagc ctaatgctgc tcgttctgag tgttgtcggc gcatctaaaa gttatgctgg
     5701 agataaactt atgagtgcga gttttgacag tgtgagagtt tattatgcca tggacaaggt
     5761 aaccggtgct gtagcgagca gtgttttcaa tgttacggtg ataacgccaa aggaggttgc
     5821 atatgggaaa tatgattctt ttgcgtataa aggcgatacg ttgcgggtta tttcctggtc
     5881 tgggtcgggt tcagctccaa ctttggtact gactgacttt gatacaatta ataactcaaa
     5941 ttgccctggc atagatacga aaatttttcg ttgtgcatat atgacgttta aaattaccgt
     6001 tgcatctgat gattatggtt gcccttggat cgcatccttt tattcctata cagatttacc
     6061 cggatttgga agctatacgg cccctacagt gcataacaca atatgcccaa cgataccggt
     6121 cgcttcatac gatatctcct ggagtgaaaa ttatgtcagc cataataagg cgttacgaat
     6181 tcagtccacg gggtcaacag tgaccaccac gctttcgacc tacctgatgg aaggcggcag
     6241 actctgtgat ggcagtaatt tttctgataa tgatggcaga ggagcatact gccgcgcggt
     6301 atcagaattg ttaaccttta cctcttacgg ttgtgataaa tcaacggtaa cggttacgcc
     6361 aactcgtcat ccggtaacgg ataaagtact tcacgatatt gtggtgaatg tgaataccag
     6421 tagtgggcaa ccgattgatt cgacgtgtcg ttttcagtat gtattgaatg aactgtagtt
     6481 cgtagtattg ctctgatgcg tcttatcagg cctacgaagt gaattgcaat ttattgaatt
     6541 atcacatatt tgtaggccgg ataaggcgtt tacgctgctc cggcatttgc tgccagcatt
     6601 cagggcaact ccggcggcaa ccactcttcc acttcaatca acaccaacaa agccgcatca
     6661 ccgccgtact ctttcggtgc ctggtgaaat gccatcacat gcggatgttg tgccagccac
     6721 agcggtgttt gctgcttcaa tatgtgcttc ccgtgaccat gcatcacgca ggcacaaaac
     6781 acatgttcgc ggcggcaggc agcaatcaat gcgcctaatt cctgcttggc ttgtagctgc
     6841 gtcagaccgt gcaaatccaa aaacaactcc ggtgaataat cgccgcggcg cagtttcttc
     6901 gcctcaaaat ggctgacatc cgggcgaaca tatttcaccg gaccttcggt attcaataac
     6961 ggctgaaact catcggaaaa ataatggctg gcatcggcct gctcctgaat caaccgtttc
     7021 accggcactt cgctaatttt cttacgctgc ggtcggtgga caatcgtgtc ctgcttaatc
     7081 ttgcgagtcc ccgccatcaa ctggcgaaac agagcctggt cctcctcgct aagtgttgtt
     7141 ttctttttca tttatctgtc tcaatttcta attttcctac agtttacccg actcgtagcg
     7201 caatcgatcg ttttaaacgt atttttcctc actcaccgcg cgtgaatgct gatttatcgt
     7261 catcttcatg gcaaactagc cgccgaaatt attgcgatca tgccctggag gaatacgtgg
     7321 ataaaatttt cgttgatgaa gcagtaaatg agctgcaaac cattcaggac atgttgcgct
     7381 ggtcggtgag ccgcttcagc gcggcaaata tctggtacgg tcacggcacc gataacccgt
     7441 gggatgaagc cgtacagctg gtgttaccat cgctctacct gccgctggat atcccggaag
     7501 atatgcgcac cgcgcgtctg acctccagcg aaaaacaccg tattgttgaa cgcgtgatcc
     7561 gccgcgtcaa cgagcgcatt ccggtggctt acctgaccaa caaagcctgg ttctgcggtc
     7621 atgaatttta cgtcgatgaa cgcgtgctgg tgccgcgctc gccgattggt gaactgatca
     7681 acaataaatt tgccggactc atcagcaagc aaccgcagca cattttagat atgtgtaccg
     7741 gtagcggctg catcgccatt gcctgtgctt atgccttccc ggatgcggaa gtcgacgcgg
     7801 tggatatctc tcccgacgcg ctggcggttg ctgaacagaa catcgaagaa cacggtctga
     7861 tccacaacgt cattccgatt cgttccgatc tgttccgcga cttgccgaaa gtgcagtacg
     7921 acctgattgt cactaacccg ccgtatgtcg atgcggaaga tatgtccgac ctgccaaacg
     7981 aataccgtca cgagccggaa ctaggcctgg catctggcac cgacggcctg aaactgacgc
     8041 gtcgcattct cggaaacgcg gcagattacc ttgcggatga tggcgtgttg atttgtgaag
     8101 tcggcaacag catggtacat cttatggaac aatatccgga tgttccgttc acctggctgg
     8161 agtttgataa cggcggcgat ggtgtgttta tgctcaccaa agagcagctg ctggcggccc
     8221 gtgaacattt cgcgatttat aaagattaag taaacacgca aacacaacaa taacggagcc
     8281 gtgatggctg gaaacacaat tggacaactc tttcgcgtaa ccaccttcgg cgaatcgcac
     8341 gggctggcgc tcggctgcat cgtcgatggt gttccgccag gcattccgct gacggaagcg
     8401 gacctgcaac atgaccttga tcgtcgtcgc cctgggacat cgcgctatac cacccagcgc
     8461 cgcgagccgg atcaggtcaa aatactctcc ggtgtttttg aaggcgttac tactggcacc
     8521 agcattggct tgttgatcga aaataccgac cagcgttctc aggattacag cgcaattaaa
     8581 gacgtcttcc gtccaggcca tgctgattac acctacgaac aaaaatacgg tctgcgcgat
     8641 tatcgcggcg gtggacgttc ttccgcccgc gaaaccgcca tgcgcgttgc agcaggggcg
     8701 attgccaaaa aatatctcgc cgagaaattt ggtattgaaa tccgcggctg cctgacccag
     8761 atgggagaca ttccgctgga aatcaaagac tggtcgcagg ttgaacaaaa tccgttcttc
     8821 tgcccggacc cggataaaat cgacgcgtta gatgaactga tgcgcgcgct gaaaaaagag
     8881 ggcgactcca tcggcgcgaa agtcaccgtt gttgccagtg gcgttcctgc cggacttggc
     8941 gagccggtct ttgaccgcct ggatgccgac atcgcccatg cgctgatgag catcaacgcg
     9001 gtgaaaggcg tggaaattgg cgacggtttt gacgtagtgg cgctgcgcgg cagccagaac
     9061 cgcgacgaaa tcaccaaaga cggtttccag agcaaccatg cgggcggcat tctcggcggt
     9121 atcagcagcg ggcagcaaat cattgcccat atggcgctga aaccgacctc cagcattacc
     9181 gtgccggggc gcactattaa ccgctttggc gaagaagttg agatgatcac caaaggccgt
     9241 cacgatcctt gtgtcgggat ccgcgcggtg ccgatcgctg aagcgatgct ggcgatcgtt
     9301 ttaatggatc acctgctacg gcagcgggcg caaaatgccg atgtgaagac tgatattcca
     9361 cgctggtaaa aaatgaacaa aaccgcgatt gcgctgctgg ctctgcttgc ctgtagcgct
     9421 agcctggcag cgacgccgtg gcaaaaaata acccaacctg tgccgggtag cgcacaatcg
     9481 ataggcagtt tttctaatgg ctgtattgtc ggcgctgaca cgttgccgat acagtccgaa
     9541 cattatcagg tcatgcgtac cgatctgcgt cgctatttcg gtcacccgga tctggtgatg
     9601 tttatccagc gtctgagtag ccaggtgagc aatctgggca tgggcacggt gctgattggc
     9661 gatatgggga tgcccgctgg tgggcgtttc aacggcggtc atgccagcca ccagaccgga
     9721 ctggatgtcg atatttttct gcaactgccg aaaacacgct ggacctccgc gcaacttttg
     9781 cgcccgcaag cactggactt agtttcccgc gacggtaaac acgttgttcc gacgctgtgg
     9841 aagccagaaa ttttcagctt gatcaaactc gccgcccagg acaaagacgt cacacgcatt
     9901 tttgttaatc cggcgattaa acaacaactt tgccttgatg cgggcaccga tcgcgactgg
     9961 ttgcgcaaag tgcgaccctg gttccagcat cgcgcgcata tgcatgtacg attacgttgc
    10021 cctgccgata gtctggagtg tgaagatcaa cctttaccgc caccaggcga tggttgcggg
    10081 gcagaactgc aaagctggtt tgaacctcca aaaccgggaa caacaaagcc tgagaagaag
    10141 actccgcctc cgttgccgcc ttcctgccag gcgctactgg atgagcacgt gatctaatgg
    10201 aaacgtttaa tagcctgttt atggtttccc cgctgttgct gggagttctc ttttttgtcg
    10261 ccatgctggc gggatttatc gactcgattg ccggtggcgg tgggttactc actattccgg
    10321 cattgatggc tgcgggaatg tctcccgcta acgcgctggc aaccaataaa ctgcaagcct
    10381 gcggcggctc tatttccgcc actatctact ttattcgccg caaagtggtt agtttaagcg
    10441 atcagaaact caatatcgcc atgacctttg tcggctcaat gagcggcgca ctgctggtgc
    10501 aatacgttca ggctgatgtc ttacggcaga ttttgcccat tctggtaatt tgtatcggcc
    10561 tctatttctt gctgatgcca aagctgggtg aagaagatcg ccagcgtcgg atgtacggtt
    10621 tacccttcgc gttaattgcc ggtggctgcg tcggttttta tgacggattc tttggtccgg
    10681 cagccggatc gttttacgca ctggcctttg ttacgctgtg cggattcaac ctcgccaaag
    10741 ccacggcgca cgccaaatta ctcaacgcaa cgtcaaacat cggcggttta ctgctgttta
    10801 ttctcggtgg caaagtgatt tgggcgacgg gctttgtcat gctggtgggg cagttcctcg
    10861 gcgcgcgcat gggttcacga ctggtgttga gcaaaggaca aaagctgatc cgcccgatga
    10921 ttgttatcgt ctcggcggtg atgagtgcca aattacttta cgatagccac ggacaggaga
    10981 tcctccactg gttggggatg aactaatgaa cagtacacac cactatgagc agttgattga
    11041 aatttttaat agctgctttg ccgatgaatt taatacccgt ctgattaaag gcgacgacga
    11101 accgatctat cttcctgctg atgcggaagt accgtataac cgaatcgtct ttgctcatgg
    11161 cttttatgcc agcgcaattc atgagatttc gcactggtgt attgccggga aagcgcgccg
    11221 tgagctggtt gatttcggct actggtattg cccggacgga cgcgatgccc aaacgcaaag
    11281 ccagtttgaa gatgttgaag tgaagccgca ggcgttcgac tggttgttct gtgtggcggc
    11341 gggatatccg tttaacgtca gttgcgacaa tctggaaggc gacttcgaac cggatcgtgt
    11401 ggtgtttcaa cggcgggtac atgcgcaggt gatggattac ctagcaaacg gcattcctga
    11461 acgcccggca cgcttcatta aagcactaca aaattattat cacacgccgg aacttacggc
    11521 ggaacagttc ccgtggccgg aagcgctcaa ctaaagccag cgccacattt tacaatgagg
    11581 aaagaagatg atcgcggagt ttgaatcacg cattctggca ttaatcgacg gtatggttga
    11641 ccatgccagt gatgatgagt tgtttgccag tgggtatttg cgtggccacc tgacattagc
    11701 catcgcagaa ctggaaagtg gtgatgacca ctccgctcag gcggtgcata cgaccgttag
    11761 ccagagtctg gaaaaagcca ttggtgccgg tgaattgtcg ccgcgtgacc aggcgctggt
    11821 gaccgatatg tgggaaaacc tgtttcagca ggcgtcacag cagtaagttg cggcgtctgg
    11881 agcaccatgc ctgatgcgac gcttatcgcg
//
LOCUS       DAPHDW010000135        11885 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.137, whole genome shotgun sequence.
ACCESSION   DAPHDW010000135 DAPHDW010000000
VERSION     DAPHDW010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11885)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11885)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11885
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.137"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..162
                     /locus_tag="RH057_003611"
     CDS             <1..162
                     /locus_tag="RH057_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3129928.1"
                     /translation="IFQWLTGIRTENIPPFNPLINEDNETVMVFNGEKIVPADGLKSQ
                     PVILPDKGK"
     gene            complement(427..1689)
                     /locus_tag="RH057_003612"
     CDS             complement(427..1689)
                     /locus_tag="RH057_003612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HDV3129929.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDS
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQDVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREQCISPFEYAKINNPLK"
     gene            complement(2093..3040)
                     /locus_tag="RH057_003613"
     CDS             complement(2093..3040)
                     /locus_tag="RH057_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001536504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HDV3129930.1"
                     /translation="MPELTSLFPDVANALDIESVAIVEKDYFVVNLLKLLKEIKPETH
                     TLVFAGGTALSKAGISLNRMSEDIDIKLVPTENFMQNGRNRKRKIRKEIVQIITDVLH
                     NSDIFSLDNENARITRDEYRYNEIPVRYPQTFAQAPCLRPFIKLELMETTLLEHPESR
                     DIYSLVTELTGNGTPVTAFPCATILSTQAEKLISMMRRTAILLRNPEQQDDESLVRHI
                     YDNYCIVREKGVNVPVLKNFVQICIQQDIERYGNQHPQFRNSPVDELKKGLDALRDDP
                     VHKLRFQRFVKPMVFGNTQIDWEEAYSCFRQTALSVLTN"
     gene            complement(3033..3428)
                     /locus_tag="RH057_003614"
     CDS             complement(3033..3428)
                     /locus_tag="RH057_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HDV3129931.1"
                     /translation="MTTKDRIQSRLNRSKRYVFTRDDFRDIAGYDQVGRALRTLVNEG
                     KLMKVGYGVYTKARRNVITGKIMPAAPGGSAAVITEALDRLNIRYSLIGATECYNKGQ
                     STQIPVSPEFKTPPRFKRVLVVGKSKINA"
     gene            complement(3497..4342)
                     /locus_tag="RH057_003615"
     CDS             complement(3497..4342)
                     /locus_tag="RH057_003615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001440175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HDV3129932.1"
                     /translation="MSDITASRPEVVTGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEISAMTESIGGKTARDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQARDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVLNVFRGLS
                     WNYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQADRLADLERMLHLFSGKPIPDNR
                     ENITIRLDDHIQSVQGKEDYEDEMFSIRYFKKGSAHITFRKPELVDRLNDIIAKYYPN
                     VLTER"
     gene            complement(4427..4624)
                     /locus_tag="RH057_003616"
     CDS             complement(4427..4624)
                     /locus_tag="RH057_003616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF957 domain-containing protein"
                     /protein_id="HDV3129933.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCESGIIFDNDEDRTDSAALLPC
                     IEQAREDIRTLRQLQLLQQNR"
     gene            complement(4642..5130)
                     /locus_tag="RH057_003617"
     CDS             complement(4642..5130)
                     /locus_tag="RH057_003617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129934.1"
                     /translation="MKLALTLEADSINVQALNMGRIVVDVDGVTLAELINVVCDNGYS
                     LRVVDESDRTSAERTPPSAALTGIRCSTAHITAKDNAWLYSLSHQTNDFGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLIVTLIRHYGVSLIHLDACAECLPGLPTF
                     DW"
     gene            complement(5127..5504)
                     /locus_tag="RH057_003618"
     CDS             complement(5127..5504)
                     /locus_tag="RH057_003618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001757701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV3129935.1"
                     /translation="MKTLPDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSACTRSQLINSIDILRARRATG
                     LMARDNYRTVNNITLGKHPGAKQ"
     gene            complement(5594..5962)
                     /locus_tag="RH057_003619"
     CDS             complement(5594..5962)
                     /locus_tag="RH057_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HDV3129936.1"
                     /translation="MSDSLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELSPRHQHTVTLYARGLTCEAD
                     TLGSCGYVYLAVYPTSETKT"
     gene            complement(6012..6656)
                     /locus_tag="RH057_003620"
     CDS             complement(6012..6656)
                     /locus_tag="RH057_003620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000086762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin of toxin-antitoxin stability system"
                     /protein_id="HDV3129937.1"
                     /translation="MSRIITTTVYTLNELSSTAREKARDEYRQHHADSNWYENVYEDF
                     REVCDIFGIDLRQRVIRLSNGRFMEEPCIWFSGFCSQGDGACFEGLWHWQPAAPRKIR
                     EYAPQDRELHRIADALQAVQKRNFWQLQAEISHRGRYCHPYSMDITVTRNSPTGQVMT
                     TDAEAAVSEVLRDLAFWLYRQLENEYDWLTSDAAVDEALLINEYTFTEAGLRAG"
     gene            complement(6675..6896)
                     /locus_tag="RH057_003621"
     CDS             complement(6675..6896)
                     /locus_tag="RH057_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HDV3129938.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(6965..7441)
                     /locus_tag="RH057_003622"
     CDS             complement(6965..7441)
                     /locus_tag="RH057_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HDV3129939.1"
                     /translation="MQQLSFLPGEMTPGERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VILAHNHPSGEVTPSKADRLITERLVQALALVDIRVPDHLIVGGNQVFSFAEHGLL"
     gene            complement(7457..7942)
                     /locus_tag="RH057_003623"
     CDS             complement(7457..7942)
                     /locus_tag="RH057_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HDV3129940.1"
                     /translation="MTTSSHNSTTPSVSVTAASGNNPPQLVATPVPDEQRISFWPQHF
                     GLIPQWVTLEPRVFGWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNV
                     MNGNRAEMSPEAAGIAACLMAYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRII
                     D"
     gene            complement(8019..8837)
                     /locus_tag="RH057_003624"
     CDS             complement(8019..8837)
                     /locus_tag="RH057_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV3129941.1"
                     /translation="MQLASRFGHVNQIRRERPLTREELMYHVPSIFGEDRHTSRSEQY
                     AYIPTITVLENLQREGFQPFFACQTRVRDQSRRGYTKHMLRLRRDGEINGQHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDKHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHNIDTDIKLNRALWVMAETLLENLR"
     gene            complement(8927..9160)
                     /locus_tag="RH057_003625"
     CDS             complement(8927..9160)
                     /locus_tag="RH057_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV3129942.1"
                     /translation="MSDCHPVLLPEGPFSREQAMAVTTAYRNVLIEDDQGTHFRLVIR
                     NAEGQLRWRCWNFEPDAGKQLNPYLASEGILRQ"
     gene            complement(9166..9843)
                     /locus_tag="RH057_003626"
     CDS             complement(9166..9843)
                     /locus_tag="RH057_003626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129943.1"
                     /translation="MNTRILFPLLFTVASFSASAGNWAVKNGWCQTMTEDGQALVMLK
                     NGTIGITGLMQGCPNGVQTLLGSRISINGNLIPTSQMCNQQTGFRAVEVEIGQAPEMV
                     KKAVHSIAERDVSVLQAFGVRMEFTRGDMLKVCPKFVTSLAGFSPKQTTTINKDSVLQ
                     AARQAYARKYDEETTETADFGSYEVKGNKVEFEVFNPEDRAYDKVIVTVGADGNATGA
                     SVEFIGK"
     gene            complement(9962..10846)
                     /locus_tag="RH057_003627"
     CDS             complement(9962..10846)
                     /locus_tag="RH057_003627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HDV3129944.1"
                     /translation="MSFSHSSLSAQVKSYLTFLPEEIRQKILEHLHGVIHYEPVIGIM
                     GKSGTGKSSLCNAIFQSRICATHPLNGCTRQAHRLTLQLGERRMTLVDLPGIGETPQH
                     DQEYRELYRQLLPELDLIIWILRADERAYAADIAMHQFLLNEGADPSRFLFVLSHADR
                     VFPAEEWNDTEKCPSRQQELSLATVTARVATLFPSSFPVLPVAAPVGWNLPAFVSLMI
                     HALPPQATSAVYSHIRGENRSEQARKHAQQTFGDAIGKSFDAAITRFSFPAWILQLLR
                     KTRDRIIHLLVTLWDHLF"
     gene            11031..>11885
                     /locus_tag="RH057_003628"
     CDS             11031..>11885
                     /locus_tag="RH057_003628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129945.1"
                     /translation="MTGRKNKCVRKNCNNQVFSVKMAVEKRLCRKHYERFCELKKRRE
                     QNPVHRLNEALPRKIKGPKSKKKLVKEREIINSRKKHKQEQGPSLTELNKKVREILKT
                     PAWRTLETRIRRNGITFKSKAELDALSELYAIYSWVSYCKRGDNYSVWFLNCPDVNIN
                     LHVCECRSSESDCYLPSRFIILPAPLRQKVKRKYSANSSGALGHNDSKSLYKTVLSVC
                     GGKRETEMFFYSLPVKTNHHPYWIKSGVVKRFPPVFGLLFYEQERLTGGVFRYVLIRF
                     YNAFKENLF"
BASE COUNT         2924 a         2770 c         3071 g         3120 t
ORIGIN      
        1 atttttcagt ggctgactgg tattcgtact gaaaatatac cgccattcaa cccgctgata
       61 aatgaagata atgaaactgt tatggttttt aacggagaga aaatagttcc tgcggacggc
      121 cttaagtcac agcctgtgat tcttccggac aagggaaaat aagagtaatg ttgagcacca
      181 tgttctgtac gatgaatttt ccactgttta tatcgattat tttgctaata accagagttg
      241 agcacccgaa aatacctgaa gaggaattac ccgcgaccag gaggttcaga tatccctgat
      301 gtcggctata aaaattttga ttacatgacg taatggaagt ggttctgtca attgccttgt
      361 ttattcatta atctcaaaga aaaaggtatt gaaaataatt cccgggctgg cagcccggga
      421 tgattactat tttaatggat tgttaatctt tgcatattca aacgggctga tacattgttc
      481 tctgttcgca tccagaaaat ctgcccacca ctgtagcatc agtcgccgtt cttccagatg
      541 ttctgcttta tgaatgtaag cagcacgtac accgttacgt tcctgatggc tcatctgacg
      601 ttcaactgca tcgcgggacc acaaacctga ttctattaac gcactacatg ccatcgcccg
      661 gaatccatga ccacagacat cctgggttgt gtcatacccc ataaccctca gggctttatt
      721 tacggtgttt tcactgattg gtttacgtgg gtcatgtgct cccgtaaaga tcagaccatt
      781 ttcaccagcc catgtctgaa gttctgccag tatggctaca acctgtttgc tgagtggcac
      841 aagatgtggt gttcgcatct tggatccccg gtgtgagaat tttacgcctg gcagaggttc
      901 gcgttcggct ggaatagtcc acatggaatt gtcaatatca atttccgacc agcgggcata
      961 tctgagttca ctggaacgaa taaaaaccag taaattcagc ataactgcca gtcgggtgac
     1021 aggctggcct ttataactgt caatacgcga cagaagttca ggcaggcgtg aaagctccag
     1081 agcagggcga tgctggcgtt ttaccgtggt caatgcacca gccatatcca gagccgggtt
     1141 atatctgatg atgccagact gtacggcgta acgcattaca gcagatattc tttgctgcag
     1201 acgactggcg atttcataaa tttgttttgc ttctgcagca cgaacaggga taagcaggtc
     1261 cggggtattg agtgttgtga tatcccggtt gccgataaag gggaaaacgt gtgtctcgag
     1321 gcttttgagt actttatcgc tgtgtgattg tgcccattgt ttattactgg catgccagcg
     1381 tcgtgcaata tgttcaaaag agagctgtaa atcagggaca acttttttaa ctcttttctg
     1441 ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc tcatcccgtt ttgtacgagc
     1501 ttcggagaga gaaacttctg gatacacacc aagtgccaga gtcttctcct tacccagaat
     1561 acgataacgg agacgccagt aacgggaacc attaggatga accagcagga acatgccggc
     1621 cccgtcagtg agtttataag ccttgtcagt gggctttgca gcccggattt ttgcgtcagt
     1681 cagtgccata gttcagcctt ctgaatgcgt taaagagggt acaaattctg tcgaactggg
     1741 gtatacccgc aattataccc acacaatgag gttgatgtgg ggagaatttg gttgagttcg
     1801 gtagaattga tttggaggaa gaacacttaa atcatggtgt tctgaaacga aaaacggacc
     1861 tccgtggagg tccgttgata tgaatttggt gcccggactc ggaatgaaac tggttgtata
     1921 acaagttgtt ttatattgat tgttttgtgt ttgttatttt tgaggccacc aatgaggccc
     1981 ccatgtgcaa ttcgaattct tttgacgcga taatccctct tttttgtgct tggctgtaat
     2041 tattttgtct ggttcgatgt gctgatgcgc ggaatcgtac ttaacgcgtt tgttagttgg
     2101 tcagaacact caacgctgtt tgtctgaaac aggagtaggc ttcttcccag tcaatctgtg
     2161 tattaccaaa taccatcggt ttaacaaatc gttgaaaacg caatttgtgt acaggatcat
     2221 cccggagtgc atccagccct ttcttaagtt catcaactgg tgagttacga aattgtgggt
     2281 gctggttacc atagcgctca atgtcctgtt gaatacatat ctgaacaaaa tttttcagta
     2341 ctggaacatt cacacctttt tccctgacaa tgcaataatt gtcgtaaatg tggcgtacca
     2401 gggactcatc atcctgttgt tcgggattgc gaagaagtat ggctgttctt cgcatcatgg
     2461 atatgagctt ttcagcctga gtggataaaa tggtggcaca ggggaatgct gttacaggtg
     2521 tgccattacc cgttaactcc gtgacgagtg agtaaatatc tctgctttcc gggtgttcca
     2581 gcagagtggt ttccatcaac tccagcttaa taaatggacg aagacagggg gcctgggcaa
     2641 atgtctgggg ataacgtacg gggatctcgt tataacgata ctcatcacgt gttatacggg
     2701 cgttttcatt atcaaggctg aaaatgtcag aattatgaag aacatccgtg attatttgca
     2761 cgatttcctt gcgaattttt cttttcctgt ttctgccatt ttgcataaag ttctctgttg
     2821 gcacgagctt tatatcaata tcttctgaca ttctgttcaa tgagatacca gcttttgaca
     2881 atgcggtacc tcctgcaaac acgagagtat gtgtttctgg ttttatttct tttagtaact
     2941 ttaaaagatt aaccacaaaa taatcttttt cgacgatggc gacagattca atatccagtg
     3001 cgttggcaac gtcaggaaat aatgatgtta attcaggcat tgattttact tttccctaca
     3061 actaacactc ttttaaagcg aggaggtgtt ttgaactccg gagataccgg aatctgtgtg
     3121 gactgacctt tattgtagca ttctgttgcg ccgatcaagc tgtatctgat attcaaacga
     3181 tccagggctt cggtgattac agcggcagag ccaccaggtg ctgcaggcat gatttttcct
     3241 gtaataacgt tgcgacgggc tttggtgtaa acaccatagc ccactttcat caacttacct
     3301 tcgtttacca gcgtacgaag agctctgcca acctgatcat agccagcgat atccctgaag
     3361 tcatcccggg tgaacacata acgctttgaa cggttcagtc gggactgaat acggtctttt
     3421 gtagtcatgc ttcctccgaa aaatattaag aatgatagca gtaatacggc atgtttaata
     3481 gcggggcagg gtcacattac ctttcggtca atacatttgg atagtatttc gcaataatgt
     3541 cattcagtct gtcaaccagc tctggtttcc tgaacgtgat gtgcgcggag cccttcttaa
     3601 agtatctgat gctgaacatc tcatcttcat agtcctcttt accctgaaca gactggatgt
     3661 gatcatccag acgaatagtg atgttttccc ggttgtcggg gatcggtttg ccgctgaaca
     3721 ggtgtagcat tctttccaga tcggcaagtc gatcagcctg ttgcccggtg ataagatgaa
     3781 acccccaccg gtcccacctc accagattgt tgacgataat tttactgcca aatttgcagg
     3841 gtgaattggt tttgtaattc cagctcagtc ctctgaaaac gttgagcacc cctcgctcaa
     3901 acacttcatc tttgttctgg tgcagttgtt caaacgtgct caggatattt gcttcgctga
     3961 tttccggaaa attatcgtac tccagtgacc ggtaccatgt atcacgggct tgtgcatcca
     4021 ttaatgacag catccctgaa cgttgcatca ggtcacgcca gagttcgcga tcgagattcc
     4081 gggtgatggc tttcattgcg gtttccggtt tctccatcag ccagcaaccg cagcgaaaat
     4141 cctgtttcat ggcccagtcc cgggcggttt taccgccaat actttctgtc attgctgata
     4201 tttcagccag ttgccggata agtgtgtcga tttgcagcag tgtactcttt cgtacagcca
     4261 gaatgtggcg gatactggtt gagcaaataa cgtccgtatg cccggtgacc acttccggac
     4321 gggaggctgt gatatctgac atcatcgttt ttccttttgg tttataaacg aaaacgccag
     4381 ctgtattcag gctggcgtca ggggaatgaa gtatggtgag taagactcac cggttctgtt
     4441 gcagaagctg tagttggcgc agggtacgga tatcttccct ggcctgttca atgcagggca
     4501 acagtgctgc tgaatccgtt ctatcctcat cgttgtcgaa gataatcccg gattcacagt
     4561 caatattgtc ctgcagccac gcaatcagaa tatccagcgc cgtttctgtc gttaatgatt
     4621 tcatgttgtg aatttccctg attaccagtc gaaagtgggc agacccggca gacattcagc
     4681 gcaggcatcc agatgaatga gactgacgcc ataatggcga atgagcgtga cgatcagacg
     4741 acgaaacgtt tttgacaggc ccaggcgttt aagtcgcaga accgggtacg accacgcatc
     4801 ggtacgtaac agatatccgc taccggtaaa atgaatccat tctgattcgc cgaagtcatt
     4861 cgtctgatgt gacagcgagt acagccaggc gttgtccttt gccgtgatat gcgcggtact
     4921 gcagcgtatg ccggtaaggg ccgcagatgg tggcgtgcgc tctgctgatg tccggtcaga
     4981 ttcatcaaca acacgaaggg agtaaccgtt atcgcagacc acgttaatca gttcagcgag
     5041 agtaacacca tcgacgtcaa cgacaatgcg ccccatgttc agtgcctgca cgttaatgct
     5101 gtcggcttcc agcgtcaggg ccagtttcat tgtttcgccc ccggatgttt acccagggta
     5161 atgttattta ccgttctgta attgtcgcgg gccatcaggc cggttgcccg gcgggcccgg
     5221 aggatatcaa tactgtttat taactgagaa cgggtacagg cgctgaatcc cggctggtcg
     5281 gtacgtacca gtgcgtattt ttcaacgaga aagttcaccg catcgcacag tgaaatgcct
     5341 gcctcaatat gctgctcaat cacacgttca tcggcaaacg gtgtgtcatt cagtgtgagg
     5401 ccgtaatgct ggtccagcag tcgggacagc agtatctgcc agatggtgac gggagacggg
     5461 cagcgcgatg cctcccgtac atgcgtgtcg ggtaatgttt tcatactgaa gattttcctg
     5521 atatgagaga ttaaattttg aggagagtgg tgctgtagcg caccgggccg gagcagacga
     5581 ttattccggt ggtttacgtt ttcgtttcgg atgttggata aacagccaga taaacgtaac
     5641 cacagctgcc gagggtgtcg gcttcgcagg tcagccctct ggcatacagc gtgacggtat
     5701 gctgatggcg ggggctgagt tcaccgctgg tgagcatgag ttccagttgt ttcatcagca
     5761 gcggaaaggc ctggtccaga tggtacgcat ccgcgtcgct gaaccggcct ctgataccgg
     5821 cgcggtcggc aaggtaatgc aaccggttac cctcctgcac cagacgtgcc ccaaaacagg
     5881 gcgtcacggt gcagggtagc ccccaccagg ggcggtcgtt gttatcgtcg ggataatttg
     5941 tttcatggag tgagtctgac acgataaaat ccttacagaa aatcggtgaa tgatagttta
     6001 acgatgacga gtcagccggc gcgcagaccg gcttcggtga aggtgtattc gttgatgagc
     6061 agggcctcat ccacagcggc gtctgaggtc agccagtcgt actcgttttc cagctgacgg
     6121 tacaaccaga aggccaggtc gcgcaggact tcgctgactg cggcttccgc atcggttgtc
     6181 attacctgac cagtcggact gttccgggtg acggtgatat ccatgctgta cggatggcag
     6241 taacgtcccc ggtgactgat ttcagcctgt aactgccaga agttacgctt ctgtaccgcc
     6301 tgcagggcat ccgcgatacg atgaagttca cggtcctgcg gggcatactc cctgattttc
     6361 cggggtgccg ctggctgcca gtgccagagc ccttcaaaac aggctccgtc cccctggctg
     6421 cagaatccgc tgaaccagat gcacggctct tccataaacc tgccgttgct gagccggata
     6481 acacgctggc ggaggtcaat cccgaaaatg tcacagacct ccctgaagtc ttcatacacg
     6541 ttttcatacc agttgctgtc agcatgatgc tgacggtact cgtcgcgggc tttttcccgt
     6601 gcggtggagg atagctcatt cagtgtatac acggtggtgg tgataattct gctcatctga
     6661 atgcccgtcc tgattcagtt cagggtgacg ctcatcagcc ggacatacgg gccaaaactg
     6721 tccttacggc gttcagcaaa cacggccagc acaccgggaa tatcctgcac ttcacgaccg
     6781 gtatacgctt cagcactgcc gtgccagcgg tatttgccgg tacagaacgg aaaaagacgg
     6841 gatgccggat gctgacggtg aatacgcatg gcttcgccac gggtgatgat tttcatagtg
     6901 ggatacctct gaagacagaa gataaaagtg aaaacaggtg tgatatggtt gtggcggtga
     6961 cgggttaaag cagaccgtgt tccgcaaagg agaaaacctg gttgccacca actatcagat
     7021 ggtccggtac ccggatatcc accagggcca gtgcctgtac caggcgttcc gtgataaggc
     7081 gatctgcctt actgggagtg acttcaccgg acgggtggtt gtgtgccagt atcacggcag
     7141 cggcattgtg gtacagggca cgtttaatca cttcccgggg atggacttcc gtgcggttga
     7201 tggtgccggt gaagagggtt tcaccggcaa tcagctgatt ctggttgttc agatacagta
     7261 cccggaactc ttcccgctcc agtcccgcca tgttcagaat cagccattcc cgtgccgcat
     7321 gcgtggaggt gaaggccaca ccgggttcat gaagatggcg gtccagggtt ttcagggccc
     7381 gcagaatgag gctgcgctcg ccgggcgtca tctctccggg cagaaaggaa agctgttgca
     7441 ttgtgcttct ctccattcag tcgatgatgc gcataatggc gctgcattcc ggatgctgca
     7501 gggcgtaatc ccgcagccgg taatagtgga ccgtcatggc gtaacactcc gtacgacagg
     7561 cgtgatgact gtacgccatc agacaggcgg caatgccggc ggcttccggg ctcatttcag
     7621 cgcggttacc gttcatgacg ttaaacagca cccatgtttc gtcatcatcg tcatccggtt
     7681 cgggtgccat aaatgcgcca ccgttgttca gggtgtacag attccagata ccaccgcagt
     7741 agtcttcaca cagacggtcc atccagccga agacacgggg ctccagggtc acccattgtg
     7801 gaatgagacc aaaatgctgc ggccagaagc tgatgcgctg ttcatcaggg acgggagtgg
     7861 caaccaactg aggcgggtta ttccctgatg cagcggtcac ggaaacagaa ggtgtggtgg
     7921 aattatgcga agaggttgtc atgatattat tccttataaa aagcaaatga atgaagaagg
     7981 ctcgggggca gggacagacg tgattcaggt aaacggcatc agcgcaggtt ctccagcagc
     8041 gtttccgcca tcacccacaa tgcgcgattg agcttaatgt cggtgtcgat gttgtgaatg
     8101 gcccgggtat ggatacgttt tccttttgca ctgcggccgg aaatcccgcc tttcagcata
     8161 ttctcctgga tggtctgata agcactccac aggtccttac cgtaatcctc ccggcgtcgt
     8221 ggcgtcagaa tgtcggcagt ggtgactggc tgatgtttgt caccataacg gtaagtcagt
     8281 gccgcctgtg ccagcgcctg gcgtgccggt ggcggcagga ccagcgactg catggcatca
     8341 cgcttttcct caatccggtc aaacacgccc accacctcgt aagccccttc aatgactctg
     8401 tccaccacat ttccccggtg cggaacacgc acttccccca gagactgacc acagacgcag
     8461 ccgttctggc agacgaacct gaagtaaccc ggcagcatct ggtagctgga ggtaccgtca
     8521 tgggagttca gcaggatgat ttcagggaca tgttgtccgt ttatctcccc gtcacggcgc
     8581 agacgcagca tgtgtttggt atacccccga cggctctggt cgcgcacacg ggtctggcag
     8641 gcgaagaatg gctgaaagcc ttcccgctgc agattttcca ggacggtgat ggtgggaatg
     8701 tacgcatact gttcactgcg ggaggtgtgc cggtcttctc caaaaatact cgggacgtgg
     8761 tacatcagtt cttcgcgtgt cagtgggcgc tcccgacgga tctgatttac atgaccaaaa
     8821 cgactggcta attgcatact ttgttcctta tgaatgagtt agcggtataa acgaaaaggc
     8881 cgcgtctccc ggagaagacg cggcctgatg gatgaaatga agacgtttat tgcctgagaa
     8941 ttccctcact ggcgagatac ggatttagct gttttccggc atcaggttca aaattccagc
     9001 accgccagcg tagctgccct tcagcattgc gaataaccag ccggaaatgc gttccctggt
     9061 catcttcaat aagcacattg cggtaagctg ttgtgacagc catcgcctgt tcgcgtgaga
     9121 aagggccttc gggcaataaa acagggtgac agtccgacat atcggctatt ttccgataaa
     9181 ttcaacgctg gctccggtgg cattaccgtc agcaccaacc gtgacgatca ctttgtcgta
     9241 cgcacggtct tcaggattga acacttcaaa ctcaacctta ttgcctttta cttcgtaaga
     9301 gccaaaatca gcggtttctg ttgtttcctc gtcgtattta cgggcgtatg cctgtcgggc
     9361 agcctgcagt acggagtctt tattaatagt ggtcgtctgt ttcggggaaa aaccggcaag
     9421 tgatgtgaca aatttcggac agaccttcag catatcaccg cgggtgaatt ccattcgtac
     9481 accaaatgcc tgtaaaacgg acacatcacg ctctgctatg gagtgaacgg cttttttgac
     9541 catttccggc gcctgtccga tttccacctc aacagccctg aatcccgtct gctgattaca
     9601 catttgtgat gtggggatca ggttaccgtt aatactgata cggctgccca ggagcgtctg
     9661 tacaccattc gggcatccct gcatcaggcc ggtaatacca atcgtgccat ttttcagcat
     9721 taccagcgcc tgaccatctt ccgtcatggt ctgacaccag ccgtttttga cagcccagtt
     9781 gccggcggag gcggagaatg atgccacagt gaacagcagg ggaaaaagaa ttctggtatt
     9841 catgactgac ctctgaaatt aaagagcgtg ttattgataa tgacagacgg tatacggcag
     9901 tggttaccgt aatccgttgt cgtgtaaagg tatacggttg ccacggggga ctccggggcg
     9961 ctcagaacag gtgatcccac agtgtgacca gcaggtggat aatgcggtcc cgggttttgc
    10021 gcagaagctg taatatccag gctggaaagc tgaaccgggt aatggcagcg tcaaagcttt
    10081 ttccgatggc gtcaccaaaa gtctgttgtg cgtgtttccg ggcctgttca gagcggtttt
    10141 ccccccggat atgtgaataa accgcgctgg ttgcctgcgg tggcagtgcg tggatcatca
    10201 gcgacacaaa tgccggaagg ttccagccga caggtgcggc aacggggagt accggaaatg
    10261 atgaagggaa cagggttgcc acccgggctg ttactgttgc cagtgagagt tcctgctgac
    10321 gggacgggca tttttctgtg tcgttccatt cttcagcagg aaatacgcga tcggcatggc
    10381 tgagaacaaa cagaaaacgc gagggatctg caccttcatt cagtaaaaac tgatgcatgg
    10441 caatatcggc agcatacgca cgttcatcag cccgcaggat ccagataatc agatccagtt
    10501 ccggcagtaa ctgacggtaa agctcccggt attcctgatc atgctgcggt gtttcaccaa
    10561 tgccgggcag atcgaccagc gtcattctgc gttcaccgag ctgcagggta agacgatgag
    10621 cctggcgggt gcagccgttc aggggatgcg tggcgcagat acgggactga aaaatggcat
    10681 tacacaggct gcttttgccg gtgccggatt tacccataat gccaatcacg ggctcgtaat
    10741 gaataacacc gtggagatgt tcaaggattt tctggcgtat ttcttcagga agaaacgtca
    10801 gatatgattt cacttgtgca gagagggaag aatggctgaa tgacataccg gtatcctgtt
    10861 gatttcactg aaccggatca cactaatccg gtttatcatt caggtaatat ggacgtgaaa
    10921 aatgatggaa tgtctgaatt acggacatga ttgttaaagt gtaatggagt atgaagtgag
    10981 tttttccgaa cgggaactaa tgacatactg cggtttcagg agatgagagg atgaccggac
    11041 gtaaaaacaa atgtgtcaga aaaaattgta acaatcaggt tttctcagta aaaatggcag
    11101 tggagaaaag actgtgcaga aaacattatg agaggttttg tgagctcaaa aaaagaagag
    11161 agcaaaatcc tgttcacaga ttaaatgaag cattgccaag aaagataaaa ggaccaaaaa
    11221 gtaagaaaaa attagtaaaa gaaagagaaa tcattaacag cagaaaaaaa cataaacagg
    11281 agcaggggcc atcgctgact gaacttaata aaaaagttcg tgagatactg aaaactccgg
    11341 catggagaac gctggaaacg agaattcgaa gaaacggaat cacatttaag tcaaaagcag
    11401 agttagatgc gctttctgaa ctttatgcaa tttattcatg ggtttcatat tgcaaacggg
    11461 gcgataatta ttctgtgtgg tttttgaatt gtccggatgt aaatataaat cttcatgtgt
    11521 gcgaatgccg gtcttctgaa agcgattgtt atctgccatc tcgttttatt attcttccag
    11581 ctccattaag gcaaaaagta aaaagaaaat actctgctaa ttcgtctggg gcactaggac
    11641 ataatgattc aaaaagtctt tataagacag ttttgtctgt ttgtggtggg aaaagagaaa
    11701 ccgaaatgtt cttttattca ttgccagtaa agactaatca tcatccttac tggattaaaa
    11761 gtggtgtggt taaaagattt cctcctgttt ttggtttgtt gttttatgaa caggaaagat
    11821 taacaggagg tgtttttcgt tatgtgttga taaggtttta taatgctttt aaagagaatt
    11881 tgttt
//
LOCUS       DAPHDW010000136        11855 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.138, whole genome shotgun sequence.
ACCESSION   DAPHDW010000136 DAPHDW010000000
VERSION     DAPHDW010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.138"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            267..1655
                     /gene="narU"
                     /locus_tag="RH057_003629"
     CDS             267..1655
                     /gene="narU"
                     /locus_tag="RH057_003629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HDV3129946.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     TVFSTAILIIPCVWLGIAVQNTNTPFGVFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QKEAVTETAAALGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1737..5477
                     /gene="narZ"
                     /locus_tag="RH057_003630"
     CDS             1737..5477
                     /gene="narZ"
                     /locus_tag="RH057_003630"
                     /EC_number="1.7.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
                     /protein_id="HDV3129947.1"
                     /translation="MSKLLDRFRYFKQKGETFADGHGQVMHSNRDWEDSYRQRWQFDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWEIQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPLIRKRLIELWREALKQHSDPVLAWASIMNDPQKCLSYKQVRGRGGFIR
                     SNWQELNQLIAAANVWTIKTYGPDRVAGFSPIPAMSMVSYAAGTRYLSLLGGTCLSFY
                     DWYCDLPPASPMTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTIAITPDYSEVAKLCDQWLAPKQGTDSALAMAMGHVILKEFHLDNPSDYFINYCRRY
                     SDMPMLVMLEPRDDGSYVPGRMIRASDLVDGLGESNNPQWKTVAVNTAGELVVPNGAI
                     GFRWGEKGKWNLESIAAGTETELSLTLLGQHDAVAGVAFPYFGGIENPHFRSVKHNPV
                     LVRQLPVKNLTLVDGNTCPVVSVYDLVLANYGLDRGLEDENSAKDYAEIKPYTPAWGE
                     QITGVPRQYIESIAREFADTAHKTHGRSMIILGAGVNHWYHMDMNYRGMINMLIFCGC
                     VGQSGGGWAHYVGQEKLRPQTGWLPLAFALDWNRPPRQMNSTSFFYNHSSQWRYEKVS
                     AQELLSPLADASKYSGHLIDFNVRAERMGWLPSAPQLGRNPLGIKAEADKAGLSPTEF
                     TAQALKSGDLRMACEQPDSGSNHPRNLFVWRSNLLGSSGKGHEYMQKYLLGTESGIQG
                     EELGASDGIKPEEVEWQTAAIEGKLDLLVTLDFRMSSTCLFSDIVLPTATWYEKDDMN
                     TSDMHPFIHPLSAAVDPAWESRSDWEIYKGIAKAFSQVCVGHLGKETDVVLQPLLHDS
                     PAELSQPCEVLDWRKGECDLIPGKTAPNIVAVERDYPATYERFTSLGPLMDKLGNGGK
                     GISWNTQDEIDFLGKLNYTKRDGPAQGRPLIDTAIDASEVILALAPETNGHVAVKAWQ
                     ALGEITGREHTHLALHKEDEKIRFRDIQAQPRKIISSPTWSGLESDHVSYNAGYTNVH
                     ELIPWRTLSGRQQLYQDHPWMRAFGESLVAYRPPIDTRSVSEMRQIPPNGFPEKALNF
                     LTPHQKWGIHSTYSENLLMLTLSRGGPIVWISETDARELTIVDNDWVEVFNANGALTA
                     RAVVSQRVPPGMTMMYHAQERIMNIPGSEVTGMRGGIHNSVTRVCPKPTHMIGGYAQL
                     AWGFNYYGTVGSNRDEFIMIRKMKNVNWLDDEGRDQVQEAKK"
     gene            5474..7018
                     /gene="narH"
                     /locus_tag="RH057_003631"
     CDS             5474..7018
                     /gene="narH"
                     /locus_tag="RH057_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HDV3129948.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQIDDYYE
                     PFTFDYEHLHSAPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRNF
                     EAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCRG
                     WRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYDA
                     DRIEEAASTEREVDLYERQCEVFLDPHDPSVIEEALKQGIPQNVIEAAQRSPVYKMAM
                     DWKLALPLHPEYRTLPMVWYVPPLSPIQSYADAGGLPKSEGVLPAIESLRIPVQYLAN
                     MLSAGDTGPVLRALKRMMAMRHYMRSQTVEGVTDTRAIDEVGLSVAQVEEMYRYLAIA
                     NYEDRFVIPTSHREMAGDAFAERNGCGFTFGDGCHGSDSKFNLFNSSRIDAINITEVR
                     DKAEGE"
     gene            7018..7713
                     /gene="narW"
                     /locus_tag="RH057_003632"
     CDS             7018..7713
                     /gene="narW"
                     /locus_tag="RH057_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HDV3129949.1"
                     /translation="MQILKVIGLLMEYPDELLWECKEDALALIRRDAPMLTDFTHNLL
                     NAPLLDKQAEWCEVFDRGRTTSLLLFEHVHAESRDRGQAMVDLLAEYEKVGLQLDCRE
                     LPDYLPLYLEYLSVLPDDQAKEGLLNVAPILALLGGRLKQREAPWYALFDALLQLAGS
                     ILSSDSVTKQVNSEERDDTRQALDAVWEEEQVKFIEDNATACDSSPLNHYQRRFSQDV
                     APQYVDISAGGGK"
     gene            7710..8390
                     /gene="narI"
                     /locus_tag="RH057_003633"
     CDS             7710..8390
                     /gene="narI"
                     /locus_tag="RH057_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HDV3129950.1"
                     /translation="MIQYLNVFFYDIYPYICATVFFLGSWLRYDYGQYTWRASSSQML
                     DKRGMVIWSNLFHIGILGIFFGHLFGMLTPHWMYAWFLPVAAKQLMAMVLGGICGVLT
                     LIGGAGLLWRRLTNQRVRATSTTPDIIIMSILLIQCLLGLSTIPFSAQYPDGSEMMKL
                     VGWAQSIVTFRGGSSEMLNGVAFVFRLHLVLGMTIFLLFPFTRLVHVWSAPFEYFTRR
                     YQIVRSRR"
     gene            8470..9363
                     /locus_tag="RH057_003634"
     CDS             8470..9363
                     /locus_tag="RH057_003634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family isomerase"
                     /protein_id="HDV3129951.1"
                     /translation="MKPQVYHVDAFTSQPFRGNSAGVVFPADNLSEAQMQLIARELGH
                     SETAFLLHNDDSDVRIRYFTPTVEVPICGHATVAAHYVRAKVLGLGNCTIWQTSLAGK
                     HRVTIEKHNDDYRISLEQGTPGFEPPLEGETREAIINALHLTEDDILPGLPIQVATTG
                     HSKVMIPLKPEVDIDALSPDLAALSAISKQIGCNGFFPFQIRPGKNETDGRMFSPAIG
                     IVEDPVTGNANGPMGAWLVHHNVLPHDGKVLRVKGHQGRALGRDGVIEVTVTIRDNQP
                     EKVTISGTAVILFHAEWAIEI"
     gene            complement(9459..10304)
                     /gene="nhoA"
                     /locus_tag="RH057_003635"
     CDS             complement(9459..10304)
                     /gene="nhoA"
                     /locus_tag="RH057_003635"
                     /EC_number="2.3.1.118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-hydroxyarylamine O-acetyltransferase"
                     /protein_id="HDV3129952.1"
                     /translation="MTPILNHYFARINWSGAAAVNIDTLRALHLKHNCTIPFENLDVL
                     LPREIQLDDQSLEEKLVIARRGGYCFEQNGVFERVLRELEFNVRSLLGRVVLSNPPAL
                     PPRTHRLLLVELEEEKWIADVGFGGQTLTAPIRLVSDLVQTTPHGEYRLLQEGDDWVL
                     QFNHHQHWQSMYRFDLCEQQQSDYVMGNFWSAHWPQSHFRHHLLMCRHLPDGGKLTLT
                     NFHFTHYENGHAVEQRNLPDVVSLYAVMQEQFGLGVDDAKHGFTVDELALVMAAFDTH
                     PEAGK"
     gene            10477..11046
                     /locus_tag="RH057_003636"
     CDS             10477..11046
                     /locus_tag="RH057_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HDV3129953.1"
                     /translation="MSRFIPIELHHASRLLNHGPTVMITSFDEQSQRRNIMAAAWSMP
                     VEFEPPRVAIVVDKSTWTRELIERNGKFGIVIPGVAATNWTWAVGSVSGREEDKFNCY
                     GIPVVKGPVLGLPLVEEKCLAWMECRLLPATSAQQKYDTLFGEVVSAAADARVFVEGR
                     WQFDDDKLNTLHHLGAGTFVTSGKRVTAG"
     gene            complement(11050..11277)
                     /gene="pptA"
                     /locus_tag="RH057_003637"
     CDS             complement(11050..11277)
                     /gene="pptA"
                     /locus_tag="RH057_003637"
                     /EC_number="5.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tautomerase PptA"
                     /protein_id="HDV3129954.1"
                     /translation="MPHIDIKCFPRELDEQQKAALAADITDVIIRHLNSKDSSISIAL
                     QQIQPESWQAIWDAEIAPQMEALIKKPGYSM"
     gene            complement(11331..11855)
                     /locus_tag="RH057_003638"
                     /pseudo
     CDS             complement(11331..11855)
                     /locus_tag="RH057_003638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT         2798 a         3076 c         3089 g         2892 t
ORIGIN      
        1 tgtaaccctt tagattcacg atctgaaggg ttgaaacatg acatacgttc aaagattaac
       61 ccccgtttga aggttagctt gttagttaca tttagtaaca catatctggc tacttaatcc
      121 ccattctgcc cccgcggtta cgctttctta tctattcttt caatcagcac ttcacccaat
      181 tcatccttaa ttctaataac tggctattgc gccttacctc ctgttttgtc ttttttcatt
      241 accaatgtgt gcatgtgagg aacaatatgg cactgcaaaa tgagaaaaat agtcgttatc
      301 ttttacgcga ctggaaacca gaaaatccgg ccttctggga aaataaagga aaacatattg
      361 ctcgaagaaa cctctggata tcagtcagtt gtctacttct tgccttctgt gtctggatgc
      421 tatttagcgc agttaccgtt aatctcaata aaatcggttt taattttact accgatcaac
      481 tctttttatt aaccgcatta ccctccgttt ctggcgcatt attgcgtgtt ccctactcct
      541 ttatggtgcc tatattcggt ggacgccgat ggacggtttt tagtactgca atcctgatta
      601 ttccttgcgt ctggctcgga attgccgtgc aaaatacgaa tactcctttt ggggtattta
      661 tcgttatcgc tttgctatgc ggttttgcag gtgcaaactt tgcttcgagc atgggcaata
      721 tcagtttctt ctttccaaaa gccaaacaag ggagcgctct tgggattaat ggcggattag
      781 gaaacttagg tgtaagtgta atgcagctgg ttgcaccgct ggtcattttt gtacctgtat
      841 ttgcctttct cggcgtcaat ggcgtaccgc aggccgacgg ttcggtgatg tcgctggcga
      901 atgccgcatg gatttgggta ccgctactgg cgattgccac gatcgccgcc tggtcaggga
      961 tgaatgatat cgccagttca cgcgcctcaa ttgccgacca gctccctgtc ttacaacgcc
     1021 tgcatctctg gctgctgagc ctgctttacc ttgccacctt cggttcgttt atcggttttt
     1081 ctgcgggttt tgccatgctg gcaaaaaccc agttcccgga tgtgaatatt ctgcgcctgg
     1141 cgttctttgg cccatttatc ggtgccatcg cgcggtcggt tggtggtgct atttccgata
     1201 agttcggcgg cgtgcgggtg acgttgatca actttatttt tatggcgatt ttcagtgccc
     1261 tgctgttcct taccttaccg ggcacaggct ccggtaattt catcgccttt tacgccgtat
     1321 ttatggggct gtttctgact gcgggtctgg gaagtggttc tactttccag atgatcgccg
     1381 tcatctttcg ccagataacc atttatcggg taaagatgaa aggcggtagt gatgagcaag
     1441 ctcagaaaga agccgtcacc gaaacggcgg cggctctggg ctttatctca gccattggcg
     1501 cagtgggcgg cttttttatt ccgcaggcgt ttggcatgtc gctcaatatg accggctctc
     1561 cggtcggcgc gatgaaagtg tttttaatct tctacatcgt ttgtgtgctg ctgacctggc
     1621 tggtttatgg tcggcggaag ttcagccaaa aataagagat cgatgcctga tgtacaaccg
     1681 tgtcgcatcc gacaaggcat cagatcccaa ttcattcctg gagcaggagt catgtcatga
     1741 gtaaactttt ggatcgcttt cgctacttca aacaaaaggg cgaaaccttt gccgatggtc
     1801 acggacaggt gatgcatagc aaccgcgact gggaggacag ctatcgccag cgttggcagt
     1861 tcgacaaaat cgtgcgttcc acccacggtg ttaactgtac aggctcctgt agctggaaaa
     1921 tctacgttaa aaatggtctg gtgacctggg aaatccaaca gaccgactac ccgcgcactc
     1981 gccctgacct gcccaatcat gaacctcgcg gctgcccgcg tggcgcaagt tactcctggt
     2041 atctttacag cgctaaccgc ctgaaatacc cgctcattcg taaacgactg attgaactgt
     2101 ggcgcgaagc cctcaagcaa cacagcgatc cggtattggc gtgggcatcg attatgaacg
     2161 atccgcaaaa gtgcctgagc tacaaacaag tgcgtgggcg cggcgggttt atccgctcca
     2221 actggcagga actaaaccag ctgattgccg ccgctaacgt ctggaccatc aaaacctacg
     2281 gcccggatcg cgttgccggt ttctcgccga tcccggcgat gtcgatggtt tcttacgccg
     2341 ccggaacgcg ttatctgtca ctgcttggcg gcacctgttt aagcttctac gactggtatt
     2401 gcgacctgcc gcccgcctcg ccgatgacct ggggcgagca aaccgacgta ccggaatctg
     2461 ccgactggta taactccagc tacatcatcg cctgggggtc taacgtaccg cagacacgta
     2521 cgccggacgc ccacttcttt accgaagtac gctacaaagg cactaaaacc atcgccatta
     2581 cccctgacta ctctgaagtg gccaaattgt gcgaccagtg gctggcaccg aaacaaggca
     2641 ctgatagcgc cctggcgatg gcaatgggcc atgtgatttt aaaagagttt catctcgata
     2701 atcccagcga ctactttatc aactactgcc gccgctacag cgacatgccg atgctggtaa
     2761 tgctggagcc tcgcgacgat ggtagctacg ttcccgggcg catgatccgc gcatctgacc
     2821 tggtggatgg actgggcgaa agcaacaatc cgcagtggaa aaccgtagca gttaataccg
     2881 caggtgaatt ggtagtgccg aacggtgcga ttggtttccg ctggggagaa aaaggcaaat
     2941 ggaatctgga atccattgcc gccggtacgg aaaccgaatt gtcgttaacc ctgctcggtc
     3001 aacatgacgc tgttgcaggc gtggccttcc cctactttgg cggcattgaa aatccgcatt
     3061 ttcgcagcgt aaaacacaat ccggtgctgg tgcgccaatt gcccgttaaa aacctgacgt
     3121 tagtcgatgg caacacctgt ccggtggtca gcgtttatga tttggtactg gcgaattacg
     3181 gcctcgatcg cgggctggaa gatgaaaaca gtgcgaaaga ttacgctgaa atcaaaccgt
     3241 acaccccagc ctggggtgag caaattaccg gcgtgccgcg ccagtatatt gaatccatcg
     3301 ctcgtgaatt tgccgatact gcccataaaa cgcatgggcg ctcaatgatt atcctcggcg
     3361 ctggcgtcaa ccactggtat cacatggata tgaactaccg tgggatgatc aatatgctga
     3421 tcttctgcgg ttgtgtcggg caaagcggtg gcggctgggc acactatgtc ggccaggaaa
     3481 aactgcgccc acaaaccggc tggttgccac tggcctttgc gctcgactgg aaccgaccac
     3541 cgcgccaaat gaacagcacc tcgtttttct acaatcattc cagccaatgg cgctatgaaa
     3601 aagtctctgc tcaggagtta ctttcaccgc tcgccgatgc cagtaagtac agcggtcatc
     3661 tgattgattt caacgttcgc gccgaacgta tgggctggct accttctgcg ccgcagctgg
     3721 ggcgtaaccc gctcgggatt aaagctgaag ccgacaaggc cggattatcc cccacagaat
     3781 ttaccgccca ggcgctgaaa tcgggcgatt tacgtatggc ctgcgaacaa ccagatagcg
     3841 gcagcaatca tccgcgtaat ttgtttgtct ggcgctctaa cctgcttggc tcctccggca
     3901 aaggccacga gtatatgcag aagtatctgc tggggaccga aagcgggatt cagggcgagg
     3961 aactcggtgc cagcgacggg atcaaaccgg aagaagtcga gtggcaaact gcggcgattg
     4021 aaggcaagct cgacctgctg gtgacgctcg acttccgcat gtccagtacc tgcctgttct
     4081 ccgatatcgt tctgcccacc gccacctggt acgaaaaaga cgatatgaac acctcggata
     4141 tgcatccgtt tattcatccg ctttctgcgg cggtcgatcc ggcgtgggaa tcacgcagcg
     4201 actgggaaat ctacaaaggt atcgccaaag cattttcgca agtgtgcgtg ggtcatcttg
     4261 gcaaagaaac cgacgtggta ttacaacccc tactgcatga ctctccggca gagctctcac
     4321 agccgtgtga agtgctcgac tggcgcaaag gcgaatgcga tctgattcca ggcaaaaccg
     4381 cgccgaatat tgtggcggtg gagcgcgact accctgctac gtatgaacgc tttacctcgc
     4441 tcgggccatt gatggacaaa cttggcaatg gcggtaaagg gatttcgtgg aatacccagg
     4501 atgaaatcga tttcctcggc aaactcaatt acaccaagcg tgatggcccg gcacaggggc
     4561 gtccgctgat tgacaccgcc attgacgctt cagaagtgat tctggcactg gcaccggaaa
     4621 ctaacggtca tgttgcagtc aaagcgtggc aggcgctggg cgagatcacc gggcgcgaac
     4681 atacccatct ggcgctgcac aaagaggacg agaagattcg ctttcgcgat attcaggcgc
     4741 agccgcgtaa aattatctcc agccccacct ggtccggtct ggaaagcgat cacgtatcct
     4801 ataacgcggg atacaccaac gttcatgagt taattccgtg gcgcacgctg tcgggacgcc
     4861 agcagctcta tcaggatcat ccgtggatgc gtgcttttgg tgaaagcctg gtggcttatc
     4921 gcccgcctat cgacacccgt agcgtcagtg agatgcgcca gataccgcca aacggcttcc
     4981 cggaaaaagc acttaacttc ctgacgccgc accagaaatg gggcattcac tcaacctaca
     5041 gtgaaaacct gctaatgctg acgctctctc gcggtggacc gattgtctgg atcagcgaaa
     5101 cagatgcccg agaactgacc attgtcgata acgactgggt ggaagtattc aacgccaacg
     5161 gcgcgctgac tgcccgcgcg gtggtcagcc aacgtgtacc gccgggcatg accatgatgt
     5221 atcacgctca ggaacgcatt atgaatattc ctggttcgga agtaactggc atgcgcggcg
     5281 gcattcataa ctcggtcacc cgcgtttgcc cgaaaccaac gcatatgatt ggcggttacg
     5341 cgcagctggc ctggggcttt aactactacg gcaccgtcgg atcgaaccgc gatgagttca
     5401 tcatgatccg caagatgaag aacgttaact ggctggatga tgaaggtcgc gatcaggtac
     5461 aggaggcgaa aaaatgaaaa tccgttcaca agtcggcatg gtgcttaacc tcgacaaatg
     5521 tatcggctgc catacctgtt cggtgacctg taaaaacgtc tggaccgggc gcgaaggcat
     5581 ggagtacgca tggtttaaca acgtcgaaac caaaccgggc attggttatc cgaaaaactg
     5641 ggaagatcag gaagagtggc aaggcggctg ggtccgcgat gtgaatggca agatccgtcc
     5701 gcgtctgggc agcaagatgg gcgtaataac caaaatcttc gccaacccgg tggtgccgca
     5761 gattgatgat tactacgaac ctttcacctt cgactacgaa catttgcata gcgcaccgga
     5821 aggcaaacat atccctactg ctcgcccgcg ttcactgatt gacggcaaac ggatggacaa
     5881 agtgatctgg ggcccaaact gggaagaact gctgggcggc gagtttgaaa aacgtgcccg
     5941 cgaccgcaac ttcgaggcca tgcaaaagga gatgtacggg cagtttgaaa acaccttcat
     6001 gatgtacctg ccgcgcctgt gcgaacactg cctcaatccc agttgcgtgg cgacctgccc
     6061 aagcggcgct atctacaaac gcgaagaaga cggcattgtg ctgattgatc aggataaatg
     6121 ccgtggctgg cgtttgtgca ttagcggttg tccgtacaaa aaaatctact tcaactggaa
     6181 aagcggcaag tcagaaaaat gtatcttctg ttatccgcga attgagtcag gccaacccac
     6241 cgtgtgctca gaaacctgcg tgggtcgcat ccggtatctg ggcgtgctgc tttacgacgc
     6301 cgaccgcatt gaggaagcgg cgagcaccga gcgcgaagtt gacctctatg aacgtcagtg
     6361 cgaagtgttc ctcgatccac acgatccctc agtgatcgag gaagccctga aacaaggtat
     6421 tccacaaaac gtgattgaag ctgcccagcg ttcgcctgtc tacaaaatgg cgatggactg
     6481 gaaactggcg ctaccgctgc accctgaata tcgcaccctg ccaatggtct ggtacgttcc
     6541 tccgctgtcg ccgattcagt cctacgcaga tgcgggcggt ttgccgaaaa gcgaaggcgt
     6601 gctgcccgcc atcgaaagcc tgcgtattcc ggtgcaatat ctcgccaata tgttgagtgc
     6661 cggcgatacc ggtccggtac tgcgggcgct gaaacggatg atggcgatgc gccactatat
     6721 gcgttcacaa accgtggaag gcgttactga tactcgtgcc atcgacgaag taggcctgag
     6781 cgtcgcccag gtcgaagaga tgtatcgtta cctcgccatt gccaactatg aagatcgttt
     6841 tgtcatcccg acgagccatc gggaaatggc gggcgatgcc ttcgcagaac gcaacggctg
     6901 cggttttacc tttggcgacg gttgtcacgg ctcggacagc aaattcaacc tgttcaacag
     6961 tagccgtatc gatgccatca acatcaccga agtgcgcgac aaagcggagg gcgaataatg
     7021 cagatcctca aagtgatcgg cctgttgatg gagtatccgg atgagctgtt gtgggaatgc
     7081 aaggaggacg cgctggcgtt gatccgccgc gacgcgccga tgcttacgga tttcacccac
     7141 aacctgctta acgcgccgct gctggataaa caggccgaat ggtgcgaagt gtttgaccgc
     7201 gggcgcacca cgtcgctgct gctgttcgaa catgttcatg ccgagtcccg cgatcgcggc
     7261 caggcaatgg tggacctgct ggcggagtat gaaaaggtcg gcctgcagct ggattgtcgg
     7321 gaactgcccg attatctacc gctgtatctg gagtatttaa gcgtactgcc tgacgatcag
     7381 gcgaaagaag gattgctcaa tgttgcgccg atcctcgccc tgcttggcgg tcgcttaaaa
     7441 caacgcgagg caccgtggta cgcgttgttt gatgctctgc tgcaactggc gggaagtata
     7501 ctttcaagtg acagtgtcac aaaacaagta aacagcgaag agcgcgatga cacccgccag
     7561 gcgcttgatg cagtgtggga agaggaacag gtgaagttta ttgaagataa cgccacggcg
     7621 tgtgacagct cgccgcttaa tcactatcag cgacgcttta gccaggatgt cgcgccgcag
     7681 tatgtcgaca tcagtgcggg aggtgggaaa tgattcagta tctgaacgtc tttttttacg
     7741 atatctaccc gtacatttgt gcgacggtgt ttttcctcgg cagctggctg cgctacgact
     7801 acgggcagta cacctggcgc gcctcctcaa gtcaaatgct cgataaacgc gggatggtta
     7861 tatggtcgaa tctgttccat atcggcattt tggggatctt cttcgggcac ctgttcggca
     7921 tgttaacgcc gcactggatg tacgcgtggt ttttgccagt ggcagcgaaa caactaatgg
     7981 cgatggtgct cggcggtatt tgcggtgtgt tgacattgat tggcggcgca ggactgctgt
     8041 ggcgcaggct gacaaatcag cgcgtgcgtg caacttccac tacgccggat attatcatca
     8101 tgagcatttt gctgatccag tgtctgctgg ggctaagtac cataccgttt tcagcacagt
     8161 atcctgatgg tagcgaaatg atgaagctgg tgggttgggc gcaaagcatt gtcactttcc
     8221 gtggtggatc gtcagaaatg cttaacggcg tagcgttcgt tttccgcctg catctggtat
     8281 tgggaatgac catcttcctg ctcttcccgt tcaccagatt ggtgcatgtg tggagcgcgc
     8341 cgtttgagta ctttactcgt cggtatcaga ttgtgcgttc gcggcgataa ttttcaccct
     8401 ggccggatag cccttcgctc tccggccact tgcaggctat gatagttctc cagccacagg
     8461 agaacgttaa tgaaaccgca ggtgtaccac gtcgatgctt ttacctcaca accgtttcgc
     8521 ggcaattctg ccggtgttgt tttccccgcc gataatctta gcgaagcgca aatgcagctt
     8581 atcgcccgcg agttaggcca ttcggaaact gcttttctgc tgcacaacga cgacagcgat
     8641 gtgcgcatcc gctactttac gccaacggtt gaagtgccga tttgcggtca cgcgacagta
     8701 gctgcgcact atgtacgtgc gaaggtgtta ggtttaggaa attgcacgat ctggcaaaca
     8761 tcgctggcag gaaaacatcg cgtgactatc gaaaagcaca acgatgatta tcgtatttcg
     8821 ctggaacaag gtacgccggg ctttgagcca ccgctggaag gtgaaacacg tgaggcgatt
     8881 atcaacgcgc ttcatctcac tgaggacgat attctgccag gcttgccaat tcaggtggca
     8941 acaacaggac actcaaaagt gatgatcccg ttgaaaccag aagtggatat cgacgccctt
     9001 tcgcctgatc ttgctgcgct gagcgctatc agcaaacaga ttggctgcaa tggtttcttc
     9061 ccgttccaga tccgcccagg caaaaacgaa accgatggtc gcatgttctc gcctgcgatt
     9121 ggtattgtgg aagatccggt gactggcaat gccaatggcc cgatgggtgc atggttggta
     9181 catcacaacg tattgcccca cgatggcaag gtgctgcgtg ttaaaggcca tcaggggcgc
     9241 gcattggggc gtgacggcgt gattgaagtg acggtgacaa ttcgtgataa ccaaccggaa
     9301 aaagtcacca tttcaggtac agcggtgatt ttgtttcatg ccgaatgggc aattgaaatc
     9361 tgaaatcgtt gcctgatgcg cttcgcctgt caggtctaca accgggctaa tgtatgccgg
     9421 ataaggcgtt tacgccgcat ccggcaacct gacataaatt attttcccgc ctccggatgc
     9481 gtatcaaacg ccgccatcac cagcgctaac tcatccacag taaagccatg tttcgcatca
     9541 tccacgccca gaccaaactg ttcctgcatc acggcatata acgacaccac atccggtaga
     9601 tttcgctgct ccaccgcgtg cccattttca taatgggtaa aatgaaaatt ggtcagcgtt
     9661 agcttgccac cgtccggcaa atggcggcac atcagcaaat gatggcgaaa atgcgactgc
     9721 ggccagtgcg ccgaccagaa attgcccatc acataatcgc tttgttgctg ctcgcagaga
     9781 tcaaaacggt acatcgactg ccaatgctga tgatgattaa actgcaacac ccaatcatca
     9841 ccctcctgca acaaccgata ctctccgtgt ggcgtggtct gcacgagatc ggaaactaaa
     9901 cgaatcggcg cggttagcgt ctgcccaccg aaaccgacat cagcaatcca tttttcctct
     9961 tccagttcca ccaacagcaa acgatgggtg cgcggcggta atgctggcgg atttgataac
    10021 actacgcgcc ctaacaagct gcgaacgtta aactccagct cgcgtaacac ccgctcaaac
    10081 acgccattct gctcaaaaca gtaaccgcca cgacgggcta tcaccagttt ctcttccagc
    10141 gattgatcat caagctgtat ttccctcggc agcaaaacgt cgaggttttc aaacggaata
    10201 gtgcaattgt gtttcaggtg caatgcacga agcgtatcaa tattgaccgc agcagctccc
    10261 gaccagttaa tacgggcaaa atagtgattc agaatgggcg tcatcaggcg tttcccttag
    10321 tggttttctc aacttataac ctggaataga cgccgcaccg gctattttgt gcgatcgcgg
    10381 acatgcatca cgtttgcaaa tcgccaatcc cgttaatttc gttacttatt gcgttacgga
    10441 ctacgcttaa aaaaaccttc ttcgcgaggc gataccatga gccgattcat ccccatagaa
    10501 ttacaccatg ccagccgtct gttaaatcat ggcccaactg tcatgatcac cagtttcgat
    10561 gagcaatccc agcggcgtaa cattatggca gcagcctggt caatgccggt ggagtttgaa
    10621 ccgccacgcg tggcgattgt ggtagataaa tcgacatgga ccagggagtt gattgaacgt
    10681 aacggtaaat ttggcatcgt tatcccgggc gttgcagcaa ctaactggac gtgggcagtg
    10741 ggaagcgtgt cgggtcgtga agaagataaa tttaattgct atggcattcc ggtggtgaaa
    10801 ggtccggtgc ttggtttgcc acttgttgaa gagaaatgcc tggcgtggat ggagtgccgc
    10861 ctgctacctg cgacttctgc gcaacaaaaa tacgacacgc tgtttggcga ggtggtatca
    10921 gcagcggcag acgcacgggt atttgtcgaa ggccgctggc agtttgatga tgataagctc
    10981 aatacgttgc atcatttagg tgctggcacg tttgttacta gcggcaagcg tgttacggca
    11041 ggttaagcat tacatgctgt aaccgggttt ctttatcaaa gcctccattt ggggcgcgat
    11101 ttcggcatcc cagatagctt gccaagattc tggttgaatc tgctgtagag caatgcttat
    11161 cgaactgtct ttactgttca gatggcgaat aataacgtcg gtaatatctg cagcaagtgc
    11221 tgctttttgt tgttcgtcca gttcacgcgg aaagcattta atgtcgatgt gcggcatgat
    11281 tgtgctcctt ttgaaagatg tcagtaaaat taacacacat tgtgaatttt cagtcctttc
    11341 cattcaaacg tgaaatcaat aagttcatca gggacatcgc aaacaatatg aatacggatt
    11401 tcttctctgc cgtgactctt ttcacaaatt gcgtaactgt catgctctgg attatttaat
    11461 tctttcagcg gaaatttttc ctcaaaggct ttatttagcc acccctggtt tcctttcacc
    11521 gcgaataaat aatcacctcc ctgtttttgt atcttctctg ctatatcttt ctggcaaccc
    11581 atcgcatcag ttgtgatgat ttttccttta atatccagca tgttaagaag ttctgggata
    11641 gctgtaatct cattagattt atcatccgtc ttgatctgtc cgatgaccag actgtgcatt
    11701 gttgagaacg cactaatgac atgaatcgct cccctgcggc gactcttatc ataagaatgc
    11761 cggagcgttt ttccatcaat tgcaatgacg tctttatcat ctgaagaatg acaatcacgc
    11821 atccagttaa taaagcactc atgaaatttt gtagg
//
LOCUS       DAPHDW010000137        11493 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.139, whole genome shotgun sequence.
ACCESSION   DAPHDW010000137 DAPHDW010000000
VERSION     DAPHDW010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11493
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.139"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            19..621
                     /locus_tag="RH057_003639"
     CDS             19..621
                     /locus_tag="RH057_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HDV3129955.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            complement(623..1144)
                     /gene="ruvC"
                     /locus_tag="RH057_003640"
     CDS             complement(623..1144)
                     /gene="ruvC"
                     /locus_tag="RH057_003640"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HDV3129956.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGIGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLARGRLR"
     gene            complement(1179..1919)
                     /locus_tag="RH057_003641"
     CDS             complement(1179..1919)
                     /locus_tag="RH057_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3129957.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAIDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            complement(1948..2400)
                     /gene="nudB"
                     /locus_tag="RH057_003642"
     CDS             complement(1948..2400)
                     /gene="nudB"
                     /locus_tag="RH057_003642"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HDV3129958.1"
                     /translation="MTDMAYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAVREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            complement(2393..4165)
                     /gene="aspS"
                     /locus_tag="RH057_003643"
     CDS             complement(2393..4165)
                     /gene="aspS"
                     /locus_tag="RH057_003643"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HDV3129959.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADAMGALRLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            4475..5041
                     /locus_tag="RH057_003644"
     CDS             4475..5041
                     /locus_tag="RH057_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HDV3129960.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADHAEALKQPVDAPSPAKVLPENWWQHPAALGATDSDIEIIKR
                     QWGAFYGTDLELQLRRRGIDTIVLCGISTNIGVESTARNAWELGFNLVIAEDACSAAS
                     AEQHNNSINHIYPRIARVRSVEEILHAL"
     gene            5038..5856
                     /locus_tag="RH057_003645"
     CDS             5038..5856
                     /locus_tag="RH057_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HDV3129961.1"
                     /translation="MIYIGLPQWSHPKWVRLGITSLEEYARHFNCVEGNTTLYALPKP
                     EVVLRWREQTTDDFRFCFKFPATISHQAALRHCDDLVTEFLTRMSPLAPRIGQYWLQL
                     PATFGPRELPALWHFLDSLPGEFNYGVEVRHPQFFAKGEEEQTLNRGLHQRGVNRVIL
                     DSRPVHAARPHSEAIRDAQRKKPKVPVHAVLTATNPLIRFIGSDDMTQNRELFQVWLQ
                     KLVQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPEIGAVPAIPQQSSLF"
     gene            5909..6304
                     /locus_tag="RH057_003646"
     CDS             5909..6304
                     /locus_tag="RH057_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HDV3129962.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRLQYRVAYGDGGFSELQSAI
                     RIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            6345..7088
                     /gene="cmoA"
                     /locus_tag="RH057_003647"
     CDS             6345..7088
                     /gene="cmoA"
                     /locus_tag="RH057_003647"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HDV3129963.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            7085..8056
                     /gene="cmoB"
                     /locus_tag="RH057_003648"
     CDS             7085..8056
                     /gene="cmoB"
                     /locus_tag="RH057_003648"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HDV3129964.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     AVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(8221..10650)
                     /gene="torZ"
                     /locus_tag="RH057_003649"
     CDS             complement(8221..10650)
                     /gene="torZ"
                     /locus_tag="RH057_003649"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HDV3129965.1"
                     /translation="MTLTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHDRIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQTSWPLILENSQAVVLWGMNPL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAAWAAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYCTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYADFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQLANACAGNSALVYIEKYTGNAPK
                     LTAFDQPAIQA"
     gene            complement(10675..>11493)
                     /locus_tag="RH057_003650"
     CDS             complement(10675..>11493)
                     /locus_tag="RH057_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HDV3129966.1"
                     /translation="FVSGKIDSDDKFETHRQEMAETVWKELKATDSATCRSCHSFDAM
                     DIASQSESAQKMHNKAQKGGETCIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNG
                     AHIYPFKTSRIGELATVNPGADLTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLA
                     LATLSEEGIKALTVNGEWQADEYGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTY
                     CAGCHAPIAADHYTVNAWPSIAKGMGARTSMSENELDILTRYFQYNAKDITEKQ"
BASE COUNT         2596 a         3078 c         2831 g         2988 t
ORIGIN      
        1 tttcaatgga atgacaacgt gaatattaat taccctgctg aatatgaaat tggagatatc
       61 gtctttacat gtataggtgc tgccttattt ggtcaaatat cagctgcatc aaattgctgg
      121 agtaatcacg tcgggatcat tatcggtcat aacggtgaag acttcctggt tgcagaaagc
      181 cgggttcccc tttcaaccat caccacgcta tctcgtttta ttaaacgttc gtctaatcaa
      241 cgctatgcta taaagcgatt agacgctggg ctgacagaac aacaaaaaca acgaattgtt
      301 gaacaggttc cttcccggct acgcaaactt taccacacgg gttttaaata cgaatcttcc
      361 cgccagttct gttcaaaatt tgtctttgat atttataaag aggcgctatg tattccggtg
      421 ggtgaaatag agacgtttgg acaactgtta aatagcaatc cgaatgcaaa actcactttc
      481 tggaaattct ggttcttagg ttctattccg tgggagcgta aaaccgtcac gccagccagt
      541 ttgtggcatc atccgggttt ggtgttgatt cacgcggagg gagttgaaac gcctcagcct
      601 gaactgaccg aggcggtata acttaacgca gtcgccctct cgccaggttc agccgcgatt
      661 cgctcatctg catcgcattc tgactgacgt gacaatgggt aatcgcgata gctaacgcat
      721 cggcggcatc cgcctgtgga ttagcgggca gtttcagcaa ggtgcggacc atatgctgca
      781 cctggctttt ttcggcacta ccaataccta ccaccgtttg ctttacctga cgtgccgcgt
      841 attcaaatac cggcaattcc tgattcaccg ccgcaacaat cgccacgccg cgcgcctggc
      901 ccagtttcag ggctgagtca gcgttcttcg ccataaagac ttgttcaatg gcgaaataat
      961 caggctggaa ctgggtgatg atttccgtca cgcccgcata gatgagcttc agacgagacg
     1021 gtaaatcatc cactttggtg cgtatgcatc cgctacccag gtaggacagt tgcctgccta
     1081 cctggcgaat tacgccatag ccggtcacgc gcgaacccgg atcaatgccg agaataatag
     1141 ccatcacgcg tctccgtttt gctgtttagc aggcctcatc agagagtcgc tgcgacctca
     1201 tcagagattt caccgttatg gtaaacttcc tgcacgtcgt cgcaatcttc cagcatatcg
     1261 atcagacgca tcagtttcgg tgcggtttct gcatccatat cagctttggt ggacgggatc
     1321 atggaaactt ccgcgctgtc tgctttcaga cctgccgctt ccagagcgtc gcgcacttta
     1381 cccatttctt cccatgcagt gtagacatca atcgcgccgt catcataggt cacaacgtct
     1441 tcagcacctg cttccagagc tgcttccatg atggtgtctt catcgccttt ctcgaaggag
     1501 atcacgcctt ttttgctgaa cagataggct acggaaccat ctgtaccgag gttaccgcca
     1561 catttgctga atgcatgacg cacttcagca acggtacggt tgcggttgtc agacagacat
     1621 tcaatcatga ttgccgtgcc gccaggaccg taaccttcgt agatgatggt ttccatgttt
     1681 gcatcatcat caccgcccac accacgtgca attgcgcggt tcagtgtgtc gcgggtcatg
     1741 ttgttagaca gtgctttatc aattgctgca cgcagacgcg ggttagcgtc cggatcgcca
     1801 ccgcccagct tagccgcggt taccagctca cgaatgattt tagtgaagat tttaccgcgc
     1861 tttgcatcct gcgcagcttt acgatgtctg gtgttggccc atttactatg acctgccata
     1921 aaaatatctc cagatagccc tgcctgttca ggcagcgtta attacaaact gttcaatcgc
     1981 ctgccggttg ctccaggact tagtgagcgc cgccgcagca ggcgcatcaa gccacttgta
     2041 agccagatgt tcagtgaaaa cgatctgccg ctcgtgtggg agcgcaagac agaaccatga
     2101 ttccgtatta cgcgtcacgc ccggcgcata gcgatgacgt aaatgtgaaa aaatttcaaa
     2161 ctctaccgtg cgctgacagt caattaaggt cagttgttca gcgacaacat caatggtgac
     2221 ctcttccttt acttcgcgca cggcagcttg cggcgcggtt tccccctctt ccacgctgcc
     2281 ggttaccgac tgccagaaat cgggatcgtc acgccgctgc aacatcagca cccgtttcgt
     2341 atcttgtgcg tagatgacca ctaagatcga aacgggacgc ttataagcca tatcagttat
     2401 tctcagcctt cttcacaacc tgaatgctca gctcagccag tgcagtcggg ttagcaaagc
     2461 tcggtgcttc agtcatcaga cacgctgccg ccgtggtttt cgggaaggca ataacgtcac
     2521 ggatattgtc ggtgccggtc agcagcatgg tcagacggtc aagaccgaat gccagacctg
     2581 cgtgcggcgg agtaccgtat ttcagagcgt cgagcaggaa gccgaatttc tcgcgctgtt
     2641 cctcttcgtt gatacccaga ataccaaaca ccgtctgctg catatcacca ttatggatac
     2701 gtactgaacc accgcccact tcgtaaccat tgatgaccat atcgtaagcg ttcgccaccg
     2761 cattttccgg tgcagctttc agttctgcag cagtcatgtc tttcggtgag gtgaacggat
     2821 ggtgcattgc cgtcaggccg ccttcaccgt cgtcttcaaa catcgggaag tcgataaccc
     2881 acagcggtgc ccatttgctt tcgtcggtca gaccaaggtc tttacccact ttcaggcgca
     2941 gtgcgcccat cgcgtcggca acaattttct tgttgtcggc accgaagaaa atcatatcgc
     3001 catcttgcgc ggcagtacga tccaggatgg cttcgatgat ttctgcatta aggaacttcg
     3061 ctaccgggct gttgatacct tccagacctt tcgcgcgttc gttaactttg atgtaagcca
     3121 gacctttcgc gccgtagatt ttaacgaagt taccgtattc gtcgatctgc ttacgggtca
     3181 gcgatgcgcc gcccggaacg cgcagagcgg caacgcgacc tttcggatcg ttcgccggac
     3241 ctgcaaatac tgcaaactca acagatttca gcagatcagc aacgtcggtc agttccatcg
     3301 ggttacgcag atccggttta tcagaaccat aacggcgttc tgcttccgca aaggtcatta
     3361 ccgggaaatc gcccagatcc acgcccttca cttccagcca caaatgacgc accagcgctt
     3421 ccatcacttc acgcacttgc ggcgcggtca tgaaagaagt ttccacatcg atctgagtaa
     3481 attcaggctg acgatcagcg cgcaggtctt cgtcacggaa gcatttaacg atctgatagt
     3541 aacggtcaaa accggacatc atcagcagct gtttgaacaa ctgtggggat tgcggcagtg
     3601 cgtagaattt acctttgtgc acacgagaag gcaccaggta gtcacgcgcg ccttccggcg
     3661 tggctttggt cagcatcgga gtttcgatgt cgaggaagcc gtggtcatcc ataaaacggc
     3721 gcaccaggct ggtgatttta gcgcgggttt tcaggcgctg cgccatttcc gggcgacgca
     3781 ggtcgaggta gcggtatttc agacgcgctt cttcggtgtt gacgtggtta gagtcaagcg
     3841 gcagaacatc tgcgcggttg atgatagtca gcgaggacgc cagcacttca atttcgccgg
     3901 tcgccatatc gcggttaata tttttttcgt cacgcgcacg tacggtgccc gtaacctgaa
     3961 tgcagaactc attacgcagt tcagaggcca gctttaacgc gtccgcacga tccggatcga
     4021 aaaatacctg cacgatacct tcgcggtcgc gcatatcgat gaagatcagg ctaccaagat
     4081 cacgacgacg gttgacccaa ccacacagag tcacctgctg ccccacgtgg gacaaacgga
     4141 gctgtccaca atattctgta cgcatgagat atcccttaac ttagctgccg gcggatgccc
     4201 cctgctgcgc aggtgaccaa gtcgcagcgt tagctgtatg tcacaactga atgaaaaaag
     4261 gcggctatta tactggaaat tctgccgcac cgtaagagcc tggcccgcgc tggaacgcct
     4321 cgttaccact ttatatcggg cctgaaatca gactctacgc cagtttgcta taaaggtgtt
     4381 gcccgaactc ataaaaatta acaaaatttg tcgttccgcc atcggctaat cgcattaagg
     4441 tgtgaggcac gattttgttt tttcaggagt cagcatgctt gaacttaatg ctaaaaccac
     4501 tgcgttggtg gtgatcgatt tacaagaagg catcttacct tttgccggtg gtccacatac
     4561 tgccgatgag gtagttaatc gcgccgggaa gctggcggcg aaatttcgcg ccagcggtca
     4621 gcccgtgttt ctggtgcgcg ttggctggtc tgccgatcac gccgaagcat taaaacagcc
     4681 ggttgatgcc ccctcccccg ctaaagtgtt gcccgaaaac tggtggcaac atccagctgc
     4741 attaggtgca actgacagtg atatcgaaat catcaaacgt cagtggggtg cgttttacgg
     4801 tacggatctg gagttgcaac tgcgccgccg aggtatcgat acaatagtgt tatgtgggat
     4861 ctcgaccaat atcggtgttg aatccaccgc ccgcaatgcc tgggaacttg gctttaatct
     4921 ggtgattgcc gaagacgcct gtagtgccgc cagcgccgag cagcacaata acagcatcaa
     4981 ccatatctac ccacgcatcg cccgtgtgcg tagcgtggaa gagatccttc acgcgttatg
     5041 atttacatcg gtctaccgca atggtcgcat cctaaatggg tgcggttggg aatcaccagc
     5101 cttgaagagt atgcccgcca ctttaactgc gtggagggca acaccacgct ttacgccctg
     5161 ccgaaacccg aggttgtcct gcgctggcgt gaacagacca cagatgactt ccgcttctgt
     5221 tttaagtttc cggcgaccat ttcgcatcag gcagcattac gacattgcga tgatttagtg
     5281 actgaatttt tgacccgcat gtcaccgttg gctccgcgca ttgggcaata ctggctgcaa
     5341 ctgcctgcca cattcggccc acgggagctg cctgcgcttt ggcattttct cgattctctc
     5401 cctggcgaat ttaattatgg tgtggaagtc cgccatccac agtttttcgc caaaggggaa
     5461 gaggaacaaa cgcttaatcg cggtttacat cagcgcggcg ttaatcgggt gattttagac
     5521 agccgcccgg ttcatgcagc acgtccacac agtgaagcta ttcgcgacgc tcaacgaaaa
     5581 aaacctaaag ttccggtaca tgctgtactg acggcgacaa atccgctgat ccgttttatc
     5641 ggtagtgatg atatgacgca aaaccgggaa ttatttcagg tctggttaca aaaattagtg
     5701 cagtggcatc agaccactac gccttatctt tttttacata cgccagacat tgcccaggcc
     5761 ccggaactgg tacataccct gtgggaagac ttacgtaaaa cgcttccaga gatcggagca
     5821 gttccggcta ttccacagca atcttctctt ttctgaattt gccacctatc atagacaggt
     5881 gccatcggcc attttaaagg gagtttgtat ggtaagcgcg ctgtatgccg ttttaagtgc
     5941 gttgttatta atgaagttct cttttgatgt cgttcgcttg cgtttgcagt accgcgttgc
     6001 ctatggtgac ggtggtttta gcgaactgca aagcgctatt cgcattcatg gtaacgcggt
     6061 ggaatatatt cccatcgcga ttgtgctgat gctgtttatg gaaatgaatg gcgcagaaac
     6121 ctggatggtg catatttgcg gcatcgtttt gcttgctggt cgtctgatgc attattacgg
     6181 ttttcatcac cgtctgttcc gctggcgacg ttccggcatg agcgccacct ggtgtgcgct
     6241 gttgctgatg gtgctggcga atctttggta tatgccctgg gagttggttt tctccctgcg
     6301 ttagcgcaca atacgccact ttctttttcc cggattttta cgttatgtct caccgcgaca
     6361 cgctattttc tgcccctatc gccagactgg gcgactggac ctttgatgaa cgggtagctg
     6421 aagtcttccc ggatatgatc cagcgttccg ttcccggcta ttccaatatt atttccatga
     6481 ttggtatgtt agccgagcgc ttcgttcaac ctggtacgca ggtttacgat ctgggttgtt
     6541 ctctgggcgc ggcgacgctc tcggtgcgtc gcaacattca tcatgataat tgcaaaatta
     6601 ttgccatcga caactccccg gcgatgattg aacgctgccg tcgtcatatt gacgcctata
     6661 aagcccctac gccagtagac gtcattgaag gtgatattcg cgatatcgcc attgaaaacg
     6721 catcgatggt ggtgctgaat tttaccctgc aattcctgga accttccgag cgccaggcat
     6781 tactggataa aatttatcaa ggactgaacc cgggcggcgc gctggtgctt tcggaaaaat
     6841 tcagtttcga agatgccaaa gttggtgaac tgttgttcaa catgcaccac gactttaaac
     6901 gtgccaacgg ttacagcgaa ctggagatca gccagaagcg cagcatgctg gaaaacgtga
     6961 tgctgaccga ttccgtggaa acccataaag cacgcctgca taaagccggt tttgagcata
     7021 gcgagctgtg gttccagtgc tttaactttg gttcactggt ggcattaaaa gcagaggacg
     7081 ctgcatgatc gactttggta acttttattc tctgattgcc aaaaatcatc tttcacactg
     7141 gctcgaaacg ctgcccgcgc agattgctaa ctggcagcgc gagcagcagc acgggctgtt
     7201 taagcagtgg tccaacgcgg tggaatttct gcctgaaatt aaaccgtatc gtctggattt
     7261 attgcatagc gtaaccgccg aaagcgaaga gccactgagc accgggcaaa ttaaacgcat
     7321 tgaaacgctt atgcggaacc tgatgccatg gcgcaaaggg ccgttctcac tgtatggcgt
     7381 caacatcgat accgaatggc gttccgactg gaaatgggat cgtgttctgc cccatctttc
     7441 tgatttaacc ggacgcacca ttctggatgt cggctgcggc agcggttatc acatgtggcg
     7501 catgattggc gcaggcgcgc acctcgcggt ggggatcgac cctacgcaac tgtttctgtg
     7561 ccagtttgaa gcggtgcgta aactactggg taacgatcag cgcgcacatt tgttaccgtt
     7621 aggtattgaa cagcttccgg cactgaaagc ctttgatacc gtcttttcga tgggcgtgct
     7681 ttaccatcgc cgttcgccgc tggagcatct ctggcagttg aaagatcaac tggtgaatga
     7741 gggtgaactg gtgctggaaa cgctggttat tgatggcgat gagaacacgg tgctggtacc
     7801 aggcgatcgt tacgcgcaaa tgcgtaatgt ctatttcatc ccttccgcgc tggcgctgaa
     7861 aaactggctg aagaagtgtg gttttgtaga tatccgcatt gtggatgtgt gcgttaccac
     7921 tactgaagag cagcgacgca ctgaatggat ggttaccgaa tctctgtccg atttcctcga
     7981 cccacatgat ccgagtaaga cagttgaagg ttatcctgcg cctaaacgcg cggtgctgat
     8041 tgcgcgcaag ccgtaaaggt ctggtattac tgccggatgc ggcttgaatg ccttatccgg
     8101 cctacaaacc cttgctaatt caatctattg cagggactat gtaggcctga taagcatagc
     8161 gcatcaggca gttttacgtt tgcataacct cagcgcccgt ttccgggcgc tattcacttc
     8221 ttatgcctga atagctggct gatcaaacgc cgttaacttc ggcgcattgc ccgtatattt
     8281 ttcgatatac accagcgcag agttaccggc acaggcattt gccagctgcg agctggggat
     8341 atccgccgtt aacacgttcg cactgccgtt tttacacaag ccattttcca gatctggcca
     8401 tgcaccttca tgcacgcata ccacgccttt tttgatcccg tcagtcacca ccgcgcctgt
     8461 cagaatctga ccacgtttgt tccacacacg caccagatca ccattcgcaa taccaaagcg
     8521 agcagcatct tcggtgtgaa tagtgattgg ttcacgatct gcaaccgcat attttttacg
     8581 cagttccgca tagttaagct gactatgtaa acggtgtgcc ggatgtgcgg tcagaagctg
     8641 caactgcttc tcgtcggcgg tacccttcca ctcatcaggc gcaagccagg ttgggtgtgc
     8701 cgggcaatcc ttatagccaa atttttccag cgttttggag taaatctcaa ttttgccgct
     8761 tggcgtaccc agcgcatttt tcaccggatc ggcgcggaaa tcagcataac gaacgtactg
     8821 ttcgttcttc tcgctgcggc gcatttcgat cagtttattt tgctgccaga aggcattaaa
     8881 catcggcata gtcacgcgtt gcgcacgagc acctttctga gcggcatcat agaaaaattt
     8941 cagccacgcc atttcatctt taccttcggt atagatctct tttccgccag gtttcagtaa
     9001 ttcagcaaga tcggcaaata cgtcaaaatc gttacgcgct tcaaattgcg gagcaacagc
     9061 ctgcttcatc ggcacaatat gctggttgct gtaatcaccg gtcatcgtca agtcattgcg
     9121 ctcaaacgat gtggtgatcg gtaatacgat atctgcatgt ttagcagctg cggtacagta
     9181 gcattcagaa acgacgatca tctccggttt ctgccatgct ttgatcagac gattagtatc
     9241 ctggtgatgg gtaaagttac cgccacccgc ccaccagatc attttgatat tcggatacgt
     9301 ttgttcttta ccgttatgct gatatttgcc accaggattt tccagcgcat cgacgatacg
     9361 ggcaacagga atcgccgtca ttccgccatc atcagcggcc tcgctggcct gtccggcaat
     9421 cgccgcagac atttccggca gcacgccgcc aacacgcgtc ggattgccac cgttggagta
     9481 atgataagag aaaccaaaac cgccgcccgg tgtaccaatc tgccctaaca tcgccgccag
     9541 cgtcaccagc atccagtgtt tctgttcacc atattgctgg cgctgaattc cccagccagc
     9601 catcagcatt gtgcgattcg ccgccatcag ttcagcgagt ttgactatct gcgcttccgg
     9661 tacgccagta atttccgccg cccaggccgc gctcttcgga gtattatcgc tcttacctgt
     9721 cagatactct tcaaactgcg gatacccggt ggtgtatttt tcaaggaaca ctttatcgtg
     9781 tttaccttgt gtcatcaggg tatgcgcaat ccctaacatc agtgccacgt cggtgcccat
     9841 attcggcgcg atccaggtgg cattatcacc aaagaactcg atggtttcgg agcggatagg
     9901 atcaatggca atcactggtt tgccagattt tttcagctga tggaagtatt ccagcccttg
     9961 ctcatcggta ctgctccagg caatttttaa ggtattcagc gggttcatcc cccacagcac
    10021 cacagcctgg ctgttttcca gaatcagcgg ccaggaggtc tgctgttcat acacctctac
    10081 agaaccgacc acatgcggca tgatcacctg tgctgccccg gttgaataat cgccgctatg
    10141 cccggaataa ccgcccgcca ggttcatata acgttgcagt aaggtttgcg ctttatgcaa
    10201 cacgccagaa gagcgccagc cgtatgatcc ggcaaaaata gccgatggtc cgttagcttt
    10261 gcgaatgcga tcgtgttgct catgaatcag ttttaatgct tgctcccagc tcacctgtac
    10321 ataagtatct tcgccacgac ctttcgccgg ttgcagtgga ttatcgagat agctttttct
    10381 taccatcgga tgctgaatac gtgccgtggt gtgtacctga tccgccgccg tagactgtaa
    10441 ggagttcggt atggttttcg ccagcgcgcc tgtcgaagaa acaatcttgc cgtccttcac
    10501 ttctacgctc atcgctcccc aacggcccgc ggtgaggatt ttaccgccct tctcttctgc
    10561 ccatgcgggt agcggtgctg ccgatgtcac caccagcgcc ccagcggcaa taccgctgtg
    10621 tttaataaat tcacgtcttg ttaatgtcat aacttcctcc ctgatcaacg aggatcactg
    10681 tttctcggta atatctttgg cgttgtactg gaagtaccgc gtcaaaatgt ccagttcgtt
    10741 ttcgctcatg ctggttcgcg cacccattcc tttggcaatg gacggccatg cattgacggt
    10801 gtaatggtca gcggcaatag gcgcatggca accagcgcaa taggtatcgt caagtttttc
    10861 agcgtattgc catagcggtt tacggtccgc taatgcggga tcggtaagcg caccctgtaa
    10921 agacgcctga cgccattgat tgccgtattc gtcagcctgc cattccccgt ttaccgtgag
    10981 cgccttgata ccttcttcac ttaatgtggc tagcgccagc cgttgacctg ccgccaggta
    11041 gagcgtgttt tcactgccct gcatttgata accctgcaac agaacgatcg gctgtttgcc
    11101 actggcatca acgacggtga gatcggcacc aggattcacg gtagccagct cgcctatgcg
    11161 agaagttttg aaaggataaa tatgtgcgcc attagtcact gaagtagcgg cctgactttc
    11221 cagctcatgc gccgcgttgt catccatttt tatttctggc ggaaaatggg caatgccttt
    11281 atgacaatcg atacaggttt cgccgccctt ttgtgctttg ttatgcattt tctgcgcaga
    11341 ttcactttgc gaggcaatat ccatggcatc aaaagaatgg caactacggc acgttgcaga
    11401 gtcagttgct tttaattctt tccatactgt ttcggccatt tcctggcgat gagtttcgaa
    11461 cttatcgtca ctgtctattt tgccgctaac aaa
//
LOCUS       DAPHDW010000138        11467 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.140, whole genome shotgun sequence.
ACCESSION   DAPHDW010000138 DAPHDW010000000
VERSION     DAPHDW010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11467)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11467)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11467
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.140"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..130
                     /gene="ghoT"
                     /locus_tag="RH057_003651"
     CDS             <1..130
                     /gene="ghoT"
                     /locus_tag="RH057_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type V toxin-antitoxin system toxin GhoT"
                     /protein_id="HDV3129967.1"
                     /translation="NISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF"
     gene            complement(249..1766)
                     /gene="lysS"
                     /locus_tag="RH057_003652"
     CDS             complement(249..1766)
                     /gene="lysS"
                     /locus_tag="RH057_003652"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HDV3129968.1"
                     /translation="MSEQETRGANEAIDFNDELRNRREKLAALRQQGVAFPNDFRRDH
                     TSDQLHEEFDAKDNQELESLNIEVSVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     SLPEGVYNDQFKKWDLGDIIGARGTLFKTQTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEVRYRQRYLDLIANDKSRQTFVVRSKILAAIRQFMVARGFMEVETPMMQVIPGGA
                     SARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYHDLIELTESLFRTLAQEVLGTTKVTYGEHVFDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDAAKALAESIGITVEKSWGLGRIVTEIFDEVAEAHLIQPTFITE
                     YPAEVSPLARRNDVNPEITDRFEFFIGGREIGNGFSELNDAEDQAERFQEQVNAKAAG
                     DDEAMFYDEDYVTALEYGLPPTAGLGIGIDRMIMLFTNSHTIRDVILFPAMRPQK"
     gene            complement(2003..3460)
                     /gene="dtpC"
                     /locus_tag="RH057_003653"
     CDS             complement(2003..3460)
                     /gene="dtpC"
                     /locus_tag="RH057_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpC"
                     /protein_id="HDV3129969.1"
                     /translation="MKTPSQPRAIYYIVAIQIWEYFSFYGMRALLILYLTHQLGFDDN
                     HAISLFSAYASLVYVTPILGGWLADRLLGNRTAVIAGALLMTLGHVVLGIDTNSTFSL
                     YLALAIIICGYGLFKSNISCLLGELYDENDHRRDGGFSLLYAAGNIGSIAAPIACGLA
                     AQWYGWHVGFALAGGGMFIGLLIFLSGHRHFQSTRSMDKKALTSVKFALPVWSWLVVM
                     LCLAPVFFTLLLENDWSGYLLAIVCLIAAQIIARMMIKFPEHRRALWQIVLLMFVGTL
                     FWVLAQQGGSTISLFIDRFVNRQAFNIEVPTALFQSVNAIAVMLAGVVLAWLASPESR
                     GNSTLRVWLKFAFGLLLMACGFMLLAFDARHAAADGQASMGVMISGLALMGFAELFID
                     PVAIAQITRLKMSGVLTGIYMLATGAVANWLAGVVAQQTTESQISGMAIAAYQRFFSQ
                     MGEWTLACVAIIVVLAFATRFLFSTPTNMIQESND"
     gene            complement(3519..5666)
                     /gene="cadA"
                     /locus_tag="RH057_003654"
     CDS             complement(3519..5666)
                     /gene="cadA"
                     /locus_tag="RH057_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase CadA"
                     /protein_id="HDV3129970.1"
                     /translation="MNVIAILNHMGVYFKEEPIRELHRALERLNFQIVYPNDRDDLLK
                     LIENNARLCGVIFDWDKYNLELCEEISKMNENLPLYAFANTYSTLDVSLNDLRLQISF
                     FEYALGAAEDIANKIKQTTDEYINTILPPLTKALFKYVREGKYTFCTPGHMGGTAFQK
                     SPVGSLFYDFFGPNTMKSDISISVSELGSLLDHSGPHKEAEQYIARVFNADRSYMVTN
                     GTSTANKIVGMYSAPAGSTILIDRNCHKSLTHLMMMSDVTPIYFRPTRNAYGILGGIP
                     QSEFQHATIAKRVKETPNATWPVHAVITNSTYDGLLYNTDFIKKTLDVKSIHFDSAWV
                     PYTNFSPIYEGKCGMSGGRVEGKVIYETQSTHKLLAAFSQASMIHVKGDVNEETFNEA
                     YMMHTTTSPHYGIVASTETAAAMMKGNAGKRLINGSIERAIKFRKEIKRLRTESDGWF
                     FDVWQPDHIDTTECWPLRSDSTWHGFKNIDNEHMYLDPIKVTLLTPGMEKDGTMSDFG
                     IPASIVAKYLDEHGIVVEKTGPYNLLFLFSIGIDKTKALSLLRALTDFKRAFDLNLRV
                     KNMLPSLYREDPEFYENMRIQELAQNIHKLIVHHNLPDLMYRAFEVLPTMVMTPYAAF
                     QKELHGMTEEVYLDEMVGRINANMILPYPPGVPLVMPGEMITEESRPVLEFLQMLCEI
                     GAHYPGFETDIHGAYRQADGRYTVKVLKEESKK"
     gene            complement(5746..7080)
                     /gene="cadB"
                     /locus_tag="RH057_003655"
     CDS             complement(5746..7080)
                     /gene="cadB"
                     /locus_tag="RH057_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadaverine/lysine antiporter"
                     /protein_id="HDV3129971.1"
                     /translation="MSSAKKIGLFACTGVVAGNMMGSGIALLPANLASIGGIAIWGWI
                     ISIIGAMSLAYVYARLATKNPQQGGPIAYAGEISPAFGFQTGVLYYHANWIGNLAIGI
                     TAVSYLSTFFPVLNDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVVM
                     TAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPKR
                     TVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVSA
                     FTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTALM
                     ILITLMNSAGGKASDLFGELTGIAVLLTMLPYFYSCVDLIRFEGVNIRNFVSLICSVL
                     GCVFCFIALMGASSFELAGTFIVSLIILMFYARKMHERQSHSMDNHTASNAH"
     gene            complement(7446..8984)
                     /gene="cadC"
                     /locus_tag="RH057_003656"
     CDS             complement(7446..8984)
                     /gene="cadC"
                     /locus_tag="RH057_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylation/transport transcriptional
                     activator CadC"
                     /protein_id="HDV3129972.1"
                     /translation="MQQPVVRVGEWLVTPSINQISRNGRQLTLEPRLIDLLVFFAQHS
                     GEVLSRDELIDNVWKRSIVTNHVVTQSISELRKSLKDNDEDSPVYIATVPKRGYKLMV
                     PVIWYSEEEGEEIMLSSPPPIPEAVPATDSPSHSLNIQNTATPPEQSPIKSKRFTTFW
                     VWFFFLLSLGICVALVAFSTLDTRLPMSKSRILLNPRDIDINMVNKSCNSWSSPYQLS
                     YAIGVGDLVATSLNTFSTFMVHDKINYNIDEPSSSGKTLSIAFVNQRQYRAQQCFMSI
                     KLVDNADGSTMLDKRYVITNGNQLAIQNDLLESLSKALNQPWPQRMQETLQQILPHRG
                     ALLTNFYQAHDYLLHGDDKSLNRASELLGEIVQSSPEFTYARAEKALVDIVRHSQHPL
                     DEKQLAALNTEIDNIVTLPELNNLSIIYQIKAVSALVKGKTDESYQAINTGIDLEMSW
                     LNYVLLGKVYEMKGMNREAADAYLTAFNLRPGANTLYWIENGIFQTSVPYVVPYLDKF
                     LASE"
     gene            complement(9733..10014)
                     /locus_tag="RH057_003657"
                     /pseudo
     CDS             complement(9733..10014)
                     /locus_tag="RH057_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290259.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(10296..11300)
                     /locus_tag="RH057_003658"
     CDS             complement(10296..11300)
                     /locus_tag="RH057_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HDV3129973.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHYFYLVEQIAELESELSQSINADDTAQRIMTIPGV
                     GPITASLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKRGDKNLRRLL
                     VQCARSFIMRLERKHGKLAEWVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA
                     "
     gene            complement(11372..11467)
                     /locus_tag="RH057_003659"
                     /pseudo
     CDS             complement(11372..11467)
                     /locus_tag="RH057_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
BASE COUNT         3164 a         2662 c         2606 g         3035 t
ORIGIN      
        1 gaacatatcc tttgtcatta tctggtttat ctcacatgag aaaacacata ttcgtttact
       61 tagtgcattc ctggtcggaa taacctggcc aatgagtctg cctgtggcat tacttttttc
      121 tctcttttag gagctgtagt tgagtggacc cgctggttat cgtgaaacga agcgtctctc
      181 ctcaaaactg gatttgcgga acgacgaacg aagcagtcgc ctgactgctt cgttcattaa
      241 agtgaaattt atttctgtgg gcgcatcgcc gggaagagaa taacgtcgcg aatagtatga
      301 ctgtttgtaa acagcataat cattcggtcg ataccaatac ccagaccagc ggttggcggc
      361 agaccatatt ccagcgcagt cacgtaatct tcgtcataga acatggcttc gtcgtcacct
      421 gcagctttag cgttaacctg ttcctggaaa cgttcagcct gatcttctgc gtcgttcaat
      481 tcgctaaaac cattaccgat ttcacgacca ccgatgaaga attcaaaacg gtcggtgatt
      541 tccgggttaa catcattacg gcgcgccagc ggtgacactt ctgccggata ttccgtaata
      601 aaggttggct gaatcagatg tgcttctgcc acttcatcaa agatctccgt gacaatacgt
      661 cccagccccc agcttttctc taccgtaatt ccgatagatt cagctaatgc tttagcagca
      721 tcaaaattat ccaggtcggc catatcggtt tctggacgat attttttgat tgcttcgcgc
      781 atggtgagtt tttcaaacgg tttgccgaaa tcaaacacat gctcgccata agtgacttta
      841 gtggtaccca gaacctcttg tgccagggtg cggaacaggg actctgtcag ttcaatcaaa
      901 tcgtggtaat ccgcatacgc catgtagagt tccatcattg tgaactcagg attatggcga
      961 acagaaatac cttcattacg gaagttacgg ttgatttcga atacccgttc aaaaccgcct
     1021 acaaccagac gtttcagata cagctccggc gcgatacgca ggtacatatc taaatccaga
     1081 gcgttatgat gggtaataaa cgggcgagca gatgccccac ctggaattac ctgcatcatc
     1141 ggggtttcta cttccataaa gccgcgcgcg accatgaact ggcggatagc ggccagaatt
     1201 tttgaacgga caacaaacgt ttgacgggat ttatcgttag cgatgaggtc cagataacgt
     1261 tgacgataac ggacttcctg atcctgcaga ccatggaatt tatctggtaa aggacgtagt
     1321 gcttttgtca gcaggcgcaa ctcagtacag tgaatggaaa gctcaccggt ttgcgtctta
     1381 aacagcgtac cgcgggcacc gataatgtca cccagatccc attttttaaa ctgatcgtta
     1441 taaacacctt ctggcaggct atctcttgca acgtacagtt gaatacggcc accgacatcc
     1501 tgcaacgtta caaaggaggc tttccccatg atacgacggg tcatcattcg gccagcaacc
     1561 gagacttcaa tgtttaagga ttccagttcc tggttatcct tcgcatcaaa ctcttcgtgc
     1621 agctggtcag aggtatggtc gcggcgaaaa tcattgggaa acgccacacc ttgctgacgt
     1681 agtgccgcca gtttttcgcg gcgatttctc agttcatcgt taaaatcaat agcctcattg
     1741 gctccccgtg tttcttgttc agacattttg gttcctctaa atccagcttt caatttagcg
     1801 tagataaaga gacagatcgg tacactgtat tatctgcctc gactattaat aactcagttt
     1861 atcttatcga aactaacgct tacctcaagt tgatatcact aataaataac catcaaaatg
     1921 gtttaaatga cttattaatc tcgatttact agtgtgatta actgaaagtt aatttattca
     1981 taaccaattg tttttacgac agttaatcgt tgctctcctg tatcatattc gtcggcgtgc
     2041 taaacagaaa acgggtagca aaggccaata cgacgatgat cgcgacacaa gccaacgtcc
     2101 actctcccat ctgagaaaag aatcgctggt aagctgcaat tgccataccg ctaatttgcg
     2161 actctgtcgt ctgctgtgcc acgacgcctg ccagccagtt ggcgaccgcg cctgttgcca
     2221 gcatataaat cccggttaat acgccagaca ttttcagacg cgtgatttgc gcaatcgcca
     2281 ccgggtcaat aaagagttcg gcaaagccca ttagcgccag cccggatatc atcacgccca
     2341 ttgacgcttg accgtcagcc gctgcatgtc gggcatcaaa tgccaacaac ataaagccac
     2401 aagccatcag cagtaagcca aaggcaaact tcagccagac gcgcaatgtt gagttgccgc
     2461 ggctttctgg cgacgccagc caggccagta caaccccagc gagcatcacc gcaattgcat
     2521 tcaccgactg gaatagtgct gtaggtactt caatattgaa tgcctgacga ttcacaaagc
     2581 gatcgataaa caagctgatg gtactgccgc cctgttgtgc cagtacccag aacaacgtcc
     2641 cgacaaacat caacaataca atttgccaaa gagcacggcg gtgttcgggg aatttgatca
     2701 tcatgcgggc aatgatttgt gcggcaatga ggcaaacgat cgccagcaaa tatccggacc
     2761 agtcgttctc cagcagcagg gtaaaaaata ctggggctaa acagagcatc accactaacc
     2821 agctccatac tggtaaggca aatttcacgc tagtgagcgc ttttttatcc atactacgcg
     2881 tggactggaa atgacgatgc ccgcttaaga aaatcaacaa accgataaac atgccgccac
     2941 ccgcaagggc aaagccaaca tgccatccat accactgagc agccaggccg caggcgattg
     3001 gggcggcgat agaaccgata ttgcccgcag catacagcag cgaaaaaccg ccatcacgtc
     3061 gatgatcgtt ctcgtcgtag agctcgccaa gcaaacagct gatgtttgat ttgaataaac
     3121 cgtagccaca aataatgatt gccagcgcca gatacaggct aaaggttgaa tttgtatcaa
     3181 tgcccagcac cacatggcca agggtcatta acagcgcgcc ggcaatcact gcagtgcggt
     3241 tgccgagcag gcggtcggca agccagccgc cgagaatagg ggtaacgtaa accagagaag
     3301 catatgcgct gaacaggctg atggcatggt tatcatcaaa accaagctga tgggtgagat
     3361 agagaatgag taaggcacgc atgccgtaaa aactgaagta ctcccagatt tggatcgcca
     3421 cgatatagta tatcgcgcgc ggctgtgagg gtgttttcat gtgttctcct tatgagcaaa
     3481 aaagggaagt ggcaagccac ttcccttgta cgagctaatt attttttgct ttcttctttc
     3541 aataccttaa cggtatagcg gccatcagcc tgacggtatg caccgtgaat atcggtttca
     3601 aagcccggat agtgagcgcc gatttcacac agcatctgca ggaactccag aaccggacgg
     3661 ctttcttcgg tgatcatttc acccggcatt accagaggaa ctcccggcgg atatggaagg
     3721 atcatattgg cgttaatacg acctaccatt tcgtcgaggt aaacttcttc ggtcataccg
     3781 tgcagctctt tctggaacgc agcatacgga gtcattacca tcgtcggcag cacttcaaat
     3841 gcgcgataca tcagatccgg cagattgtgg tgaacaatca gtttgtggat attctgagcc
     3901 agttcctgaa tacgcatgtt ttcatagaat tcaggatctt cacgatacag agacggcagc
     3961 atgtttttca cacgcaggtt caggtcgaac gcacgtttga agtcagtcag agcacgcagc
     4021 aggctcagtg ctttggtctt atcgataccg atgctgaaca ggaacagcag gttatacgga
     4081 ccggttttct caacaacgat gccatgttcg tcgaggtatt tcgccacgat gctggccgga
     4141 ataccaaagt cgctcatggt gccgtctttt tccatccccg gagtcagcag ggtgactttg
     4201 atcgggtcaa gatacatgtg ctcgttatcg atgtttttga agccgtgcca ggtgctgtca
     4261 gaacgcagcg gccagcattc agtcgtatcg atatgatccg gctgccatac atcaaagaac
     4321 cagccatcag attccgttct cagacgtttg atctctttac ggaatttgat cgcacgttca
     4381 atggaaccgt tgatcagacg tttacctgca ttgcctttca tcatcgccgc agcggtttca
     4441 gtggacgcca cgataccgta gtgcggagaa gtggtggtgt gcatcatgta ggcttcgtta
     4501 aaggtttctt cgtttacgtc acctttaacg tggatcatgg aagcctgaga gaacgccgcc
     4561 agcagtttgt gagtggactg ggtttcgtaa atcactttcc cttctacacg gccaccgctc
     4621 ataccgcatt taccttcgta aatcggtgag aagttggtgt aaggcaccca cgcggagtca
     4681 aagtggatgg atttcacatc cagtgttttc ttgatgaagt cggtgttgta cagcagacca
     4741 tcataggtag agttggtaat tacagcatgt accggccagg ttgcgtttgg tgtttctttc
     4801 acgcgcttag caatggtagc gtgctggaat tcactctgtg ggataccacc aagaataccg
     4861 taagcgttac gggtcgggcg gaaatagatt ggcgtaacat cgctcatcat catcaggtgg
     4921 gtcagcgatt tgtggcagtt acggtcaatc agaatggtgc tgcctgccgg agcagagtac
     4981 atgccaacaa ttttgttcgc agtggaagta ccgttggtca ccatgtagct gcggtctgcg
     5041 ttaaagacgc gagcgatata ctgttctgct tctttgtgtg gaccactgtg atccagcaga
     5101 gaacccagtt cagatactga aatggaaata tcagatttca tggtattcgg accaaagaaa
     5161 tcatagaaca ggctacctac cgggcttttc tggaatgcag taccgcccat gtgaccagga
     5221 gtacagaaag tatatttacc ttcacgaaca tatttaaaca gtgctttagt cagcggaggc
     5281 agaatagtgt tgatatattc gtcagtggtc tgcttgatct tattagcaat atcttcagca
     5341 gcacccagcg catattcaaa gaagctaatc tgtaaacgca ggtcattcag gcttacatcg
     5401 agagtggaat acgtattagc gaacgcgtac aacggcaggt tctcgttcat tttgctaatt
     5461 tcttcgcaca gctcgagatt atatttatcc cagtcaaaaa taacgccgca cagacgcgca
     5521 ttgttttcga tcagttttaa taagtcgtca cggtcgttcg ggtaaacaat ctggaagttc
     5581 agacgttcaa gcgcgcgatg aagttcacgg atgggttctt ctttaaaata aacccccatg
     5641 tgattcaata ttgcaataac gttcatagtc atatctccag gtaaaaaagg cccctcccaa
     5701 cacatgggac aaaatgaaag gaggagcctc ggaaaatact tttaattaat gtgcgttaga
     5761 cgcggtgtgg ttatccattg agtggctctg gcgctcgtgc attttgcgag cgtagaacat
     5821 caggataatc aggctgacga tgaaggtacc tgccagctcg aaggagcttg cgcccatcag
     5881 cgcgatgaag cagaacacgc aacccagtac agagcagatc aggctgacaa agttgcggat
     5941 gttaacgcct tcaaaacgaa tcaggtcaac gcaagagtag aagtacggca gcatagtcag
     6001 cagtactgcg ataccggtca gttcaccgaa caggtcagat gctttaccac cggcagagtt
     6061 catcagagtg atgaggatca tcagggcagt cattttcact gcagccagca gcagaccttt
     6121 tttcggaata ccgttgctgt cgacttcacc ataaactttc gggaagttac cgtcgttagc
     6181 ggcacgtaca cctgcctggc ctaccaacat catccaggag cccagagaag tcaggcacgc
     6241 aaaggcggtg aatgcagaaa ccagcggcgc agcccagtta ccgaggatgg ttgaagcact
     6301 gattgcaaac ggagcaccgg aagctgccat tacagaagat ggatacatac cggaaagcac
     6361 ctgagtcgca gcgatgtaaa caatacctgc taaaccagta cccagcatgg ttgccagcgg
     6421 aacggtacgt ttcgggtttt taaccatacc agtacttact gctgcggatt caacacccac
     6481 gaaggcccac aggcagagca gaatactttt aatgatcgca tgaccatcag tggtatccgc
     6541 agtattccag ttagctgcat aagttgccgc atcaaaccaa tgccagccaa caatagcagt
     6601 catcaccaca ggaataagaa ccagcaccag accaatagtg gttaaacggc ttacccaagt
     6661 accgccgagc atatttacaa aggtaaatac ccagacgata gcaatacagg cgatacccgc
     6721 cggaacagga tcatttaata ctgggaagaa ggtggaaaga taagataccg cggtaatacc
     6781 aatcgccagg ttaccaatcc agttagcatg gtaataaaga acacctgtct gaaaaccaaa
     6841 tgcaggggaa atttctccgg cataagcaat tgggccacct tgttgcgggt tttttgttgc
     6901 cagtcgggca tatacatacg ccagcgacat tgcaccaata atagagataa tccaacccca
     6961 gatagcaata ccaccgatac ttgctaggtt cgcaggtaat aatgcaatac cgctccccat
     7021 catattaccg gcaacaacac cggtacaggc aaatagcccg atcttcttgg cagaactcat
     7081 gctcttctcc taatttcatt tttgaatttg gagtccgggt catgatgtat aactatttcc
     7141 tgaccagacc aaactggcgc taagaatact cacgaaaaaa ggattaatcc taaagattag
     7201 gtgaaataac acaaaagttt ctgtaagtga gaacttgagg ttttttatta acacatcagg
     7261 atcgcaagtt gatatcatga aaagataaac attcaatgtt tacaatggat tgcgggatat
     7321 tctctggtta aatttatgta ataaaaatta tgcggcaaat aaattgccgc aacatattat
     7381 accaacagaa acatacaaaa actcaacaac aaatattccc gagcataaat caacccggga
     7441 gttacttatt ctgaagcgag aaatttgtcg agataaggta caacataagg aacagaagtc
     7501 tggaatatac cattttcaat ccagtaaagg gtgtttgccc ctgggcgtaa attaaaggcg
     7561 gtgagatatg catcagccgc ttcccggttc atccccttca tttcataaac cttgccaagc
     7621 aacacataat ttagccagga catttcaaga tcaatgccag tatttatcgc ctggtaagac
     7681 tcatctgttt taccttttac cagagcactg accgctttta tttgatatat aatggatagg
     7741 ttattcaatt ccggtagtgt aacaatgttg tctatttctg tgttcagtgc tgctaattgt
     7801 ttttcatcta aagggtgttg agaatggcgc acgatatcaa ctaatgcttt ttctgctctc
     7861 gcgtaggtaa attctgggga tgattgaaca atctcaccta ataattcact ggcacggttc
     7921 aatgatttat catcgccatg cagtaaataa tcatgtgcct gataaaaatt agttaataac
     7981 gcaccacgat gaggcaaaat ttgctggagc gtctcctgca ttcgttgtgg ccacggttgg
     8041 tttaatgctt ttgataaact ctccagtaaa tcattttgaa tcgccagctg attaccgtta
     8101 gtgatgacat aacgtttatc cagcatggtt gaaccatctg cattgtctac caattttatc
     8161 gacataaagc attgttgagc acggtattgg cgctgattaa caaaggcaat agataatgtt
     8221 ttaccggaac tgctcggttc atcaatgttg tagttgattt tgtcatgcac cataaaggtg
     8281 gagaaggtgt taagtgatgt cgccaccaaa tcacccacgc ctatcgcgta agagagctga
     8341 tacggggaac tccagctgtt acaactttta tttaccatat taatgtcaat atcgcgtgga
     8401 ttgagcaaaa tacgcgattt gctcatagga agacgtgtat caagagttga aaacgctacc
     8461 agtgctacac agatacctaa cgacaacagg aaaaaaaacc atacccaaaa ggtagtgaat
     8521 cgtttgcttt taattgggga ttgttcaggt ggcgttgcgg tgttttgaat gttaagactg
     8581 tgggagggag aatctgtggc aggaaccgcc tctggtatag ggggaggcga agatagcatt
     8641 atttcctctc cctcttcttc gctgtaccag ataaccggca ccattaattt atagccgcgc
     8701 tttggtacag tagcgatata gacaggacta tcttcatcat tatcttttaa tgacttacgt
     8761 agttctgaga tactctgcgt cacaacatga ttggtgacaa tacttctctt ccagacatta
     8821 tcgataagtt catccctgct aagtacttcg ccactgtgtt gagcaaagaa aaccagaaga
     8881 tcgattaatc tcggctcaag ggtaagttga cgcccattgc ggctaatttg gtttatggac
     8941 ggagtaacaa gccattcgcc aacgcgaact acaggttgtt gcataataga aactcattcg
     9001 aaaagggaat gatgcaatga taattgccac aacctatttt taccatctat agatgggttt
     9061 atttacatat tattggtgaa tgtaagacgt tatttttacc agccataaac ttcttgatta
     9121 catagtatta cgaaaggatt ttactgagaa ccagaagtaa tattccttac catcaaaatt
     9181 catcatcttt gccaaagaaa aatgttcaga aaataatcca tggaaaattg tccggagcgc
     9241 ttactatttt aatggattgt tagtctttgc atgagcaagc ggactgatac atttctcttt
     9301 gttctcattc agaaaatctc atcagtcggc gttcagaccg gtgttgtgct ttatggatgc
     9361 gttacgctcc tgatgacgtc atttgacgtt caacaacatc acggggccgc acgacatttc
     9421 acgtcagtta gtgctatcgc tcaggaacaa attttcccga attgggatat gcccgcaaat
     9481 tgctggtgat gaggggagaa tctggttgag ttcgggagaa ttgatttgga ggcagaacgc
     9541 ttaaatcgtg gcgtcctgaa acgaaaaacg gacctccgtg gaggtccgtt tatatgaatt
     9601 tggtgccgga ctcggaatcg aagaatgctt ctatcatgtt gatattagtt tattttgttt
     9661 ggtttggtga aatatgtacc ctcttttgta cccgcagtcc cttttgatat cgaaagggaa
     9721 tcaaactcgg ggtcaggctg agagcacaga aggaaagtgc ctggctatta tatcgttaat
     9781 tctgtcaatc agctccagcc ttttgaaagt gatgtgtgca gatcccttct taaagtattt
     9841 aatgatgaac atctcatctt catagcgtcc tttaccctga acggactgta tgtggtcatc
     9901 cagattaatg gtgatattcc ctcggttgtc ggggatcggt ttaccgctga gcagatgcaa
     9961 cattctttcc aggtcagcca ggcgatctgc ctgcattcca ctgataagat gaaatctatg
    10021 gtcacccccg tttttgcaac actgattttt gattaatggt tggcttgctt aaatctatcc
    10081 ggcgtctctg tgggcaagga tgcccgcgcc ccgatgagtt ccgcacctga taatcctaaa
    10141 aatcccctcg gcttcagtga gccatttcat ctgttaggtt tttaatcagg tcgatttgcc
    10201 gttcatgtca tcaataatca gtcttcgcaa aaccagaaag gtaacttgta tcttattgtt
    10261 taaaataaac taattgagtt atatcagcat ctatatcagg cctgatattc ttttttctga
    10321 gtagtgattg cccaggcaat tcgagccagt ttatttgcca gagcgcaggt aaccacattc
    10381 gaatgcttct tgtttagttg ctcccgaacc cactcagcaa gttttccatg cttgcgttca
    10441 agtcgcatta tgaatgagcg ggcacactga accagtaacc ggcgtaaatt tttatcacct
    10501 cgcttactga tacccaacag agtcgatttt cctccagtgc tatactgccg gggaacaagt
    10561 cctgtggaag ctgcaaaatc cctgctgcat gagaactgtt tcccgtcacc aagttgcgac
    10621 gatagtaagc tggcagtaat cggaccaaca ccaggaattg tcataatgcg ctgagcggta
    10681 tcatcagcgt tgattgactg acttaattct gattccagct ctgctatttg ctcaacaagg
    10741 taaaagtagt gggtatgcaa cctcaccaaa aggcggctca gatattcggg gatttcgtgt
    10801 tcagccaaca cgagagacaa ccgttttatt acagcatcac ctatcggcag gctgattcca
    10861 aattccagca agaaaccgtg gatctggttg gttgttttca ccttatctct gatgagcgat
    10921 tctctgaccc ggtgtaacgc cctcatggcc tgctgggtct cagttcttgg ctggacaaaa
    10981 cgcattgatg gtcgtgaagc cgcttcgcat atggcttctg catcaacaaa atcattctta
    11041 ttacttttga caaacgggcg aacgaactgt ggagaaatca gcttagtttc atgcccaaaa
    11101 tcagagatac agcgagccat aaagtgtgca cccgcacagg cttccattac aacaactgag
    11161 ggcggacatg tcgccagaaa ctgcatcagt ttagtacggg taaatttttt acgcagaaga
    11221 gccttaccag acttgtcttg acaatgaatg tggaaggagt gctttccgag atcgatacca
    11281 ataagtgtga tgttctgcat gatggtctct ccgttaaagg gatttacctt aacagcgtac
    11341 tgctgttaag gtggggggga ccatatcatt atccccactg gtcccatctc accagattgt
    11401 tgacgataat tttactgcca aatttacagg gtgagttggt tttgtaatcc cagctcagcc
    11461 ctttgaa
//
LOCUS       DAPHDW010000139        11376 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.141, whole genome shotgun sequence.
ACCESSION   DAPHDW010000139 DAPHDW010000000
VERSION     DAPHDW010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11376
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.141"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            342..923
                     /gene="mdaB"
                     /locus_tag="RH057_003660"
     CDS             342..923
                     /gene="mdaB"
                     /locus_tag="RH057_003660"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HDV3129974.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYDVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGGLVQGKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            954..1268
                     /locus_tag="RH057_003661"
     CDS             954..1268
                     /locus_tag="RH057_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HDV3129975.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(1316..3208)
                     /gene="parE"
                     /locus_tag="RH057_003662"
     CDS             complement(1316..3208)
                     /gene="parE"
                     /locus_tag="RH057_003662"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HDV3129976.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQDLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(3237..3818)
                     /gene="yqiA"
                     /locus_tag="RH057_003663"
     CDS             complement(3237..3818)
                     /gene="yqiA"
                     /locus_tag="RH057_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HDV3129977.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(3818..4645)
                     /gene="cpdA"
                     /locus_tag="RH057_003664"
     CDS             complement(3818..4645)
                     /gene="cpdA"
                     /locus_tag="RH057_003664"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HDV3129978.1"
                     /translation="MESLLTLPLAGEARVRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(4670..5092)
                     /locus_tag="RH057_003665"
     CDS             complement(4670..5092)
                     /locus_tag="RH057_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HDV3129979.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(5093..5722)
                     /gene="nudF"
                     /locus_tag="RH057_003666"
     CDS             complement(5093..5722)
                     /gene="nudF"
                     /locus_tag="RH057_003666"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HDV3129980.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            5927..7408
                     /gene="tolC"
                     /locus_tag="RH057_003667"
     CDS             5927..7408
                     /gene="tolC"
                     /locus_tag="RH057_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HDV3129981.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLAREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            7556..8227
                     /locus_tag="RH057_003668"
     CDS             7556..8227
                     /locus_tag="RH057_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HDV3129982.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVFMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            8233..9393
                     /locus_tag="RH057_003669"
     CDS             8233..9393
                     /locus_tag="RH057_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HDV3129983.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDDIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(9431..10219)
                     /gene="ygiD"
                     /locus_tag="RH057_003670"
     CDS             complement(9431..10219)
                     /gene="ygiD"
                     /locus_tag="RH057_003670"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HDV3129984.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPVLAQHLVELLAPVPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWAMSFNEYVKANLTWQGAVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGAWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            10362..11135
                     /gene="zupT"
                     /locus_tag="RH057_003671"
     CDS             10362..11135
                     /gene="zupT"
                     /locus_tag="RH057_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HDV3129985.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQPLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(11193..11363)
                     /locus_tag="RH057_003672"
     CDS             complement(11193..11363)
                     /locus_tag="RH057_003672"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129986.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
BASE COUNT         2822 a         3061 c         2812 g         2681 t
ORIGIN      
        1 gtttgaattt tatgctttaa catatatcag tacgctcata aaaatccatt tataattatc
       61 aacatgacac gttgatattt tcatctttta cttattcaaa gccatatttt caatcccaaa
      121 aaaaaccgaa ccaaacgtgt aaattattcc ggtgcgagta agaattatga ggaatggcta
      181 tcagtattgt cattttcaga aaatatttat cctgcatcgg tgagtcagag caagatcaga
      241 cttttgctaa attcgcaaaa gactttgcac attttgctaa tttcaccgta ccgctctgtg
      301 acgtactata gtcggcaaac gtctcacctt gaggttaaaa aatgagcaac atcctgatta
      361 tcaacggcgc gaaaaaattc gcccactcca atggtcaact gaacgacacc ctgaccgaag
      421 tcgctgatgg cacactgcgc gacctcgggc atgatgtccg catcgttcgc gccgacagcg
      481 actacgatgt caaagcggaa gtacaaaact ttctctgggc tgatgtggtg atctggcaga
      541 tgccaggctg gtggatgggc gcgccgtgga cagtgaaaaa atatattgat gatgtattca
      601 ccgaaggtca tggaacgctg tatgccagcg atggccgtac ccgcaaagat ccgtcaaaaa
      661 aatacggttc cggcggcctg gtacagggca aaaaatatat gctttctctg acctggaacg
      721 caccaatgga agccttcacc gaaaaagatc aattcttcca cggcgttggc gtcgacggtg
      781 tgtatctgcc gttccataaa gcaaaccaat tccttggcat ggaaccgctg ccgacattta
      841 tcgctaatga cgtgataaaa atgcctgatg ttccccgcta tactgaagaa tatcgcaagc
      901 atcttgtgga aatttttggt taactagagc tcaggcttta gaaggagtta accatgctta
      961 ccgtaatcgc agaaatccgt actcgtcctg gtcaacatca ccgtcaggcg gtattggatc
     1021 agtttgctaa aatcgttcca accgtactga aagaagaagg ttgccacggc tatgcgccaa
     1081 tggtggattg cgctgctggc gtgagtttcc agtctatggc accggattct atcgtgatga
     1141 ttgagcagtg ggaaagcatc gcgcatcttg aagcgcatct gcaaaccccg cacatgaagg
     1201 cgtacagcga agccgtaaaa ggcgacgtgc tggagatgaa tatccgcatc ctgcaacctg
     1261 ggatttaaac ctgccttgtt tgcccggcca tcctgaccgg gcaatgttct ttcctttaaa
     1321 cctcaatctc cgccatgtcg cctttctctt gcaaccagtt gcggcgatct tccgagcgtt
     1381 tcttcgccag cagcatatcc atcatcgcgt cagtacgctg atcgtcttca tcatcgatag
     1441 tcaactgcac cagacggcga gtgttcggat caagcgtggt ttcacgcaat tgcatcgggt
     1501 tcatttcccc cagaccttta aaacgctgga cgttcggctt gcctttcttg cgttttaatt
     1561 gctcaagtac gccctctttc tcttcttccg tcagcgcgta ataaacctct ttcccgagat
     1621 caatacggta gagcggtggc agtgcgacgt aaacgtgacc gtgtttcacc aacgcgcgga
     1681 agtgttttac gaacaaagcg cagagcagcg tggcaatgtg cagaccatca gagtccgcat
     1741 ccgcgaggat acagattttg ccataacgaa gctggctcag atcgtcgctg tcaggatcga
     1801 taccgatcgc taccgaaata tcatgcactt cctgcgaggc cagcacttcg tcggaagaaa
     1861 cttcccaggt gttaaggatc ttacctttca gtggcatgat cgcctgatat tcgcgatcgc
     1921 gcgcctgctt ggcagatccg cctgcggagt caccttccac aaggaacagt tcggtacggt
     1981 taagatcctg cgcggtacag tcagccagtt tgccaggcag tgctgggccg ctggtcagct
     2041 ttttgcgcac cactttttta gccgcacgca tacggcgctg ggcgctggaa atcgccatct
     2101 ccgccagcag ctccgccgcc tgaacgttct ggttcagcca caggataaag gcatctttca
     2161 ccacgcctga aacgaatgcc gcgcattgac gcgaagagag acgctctttg gtctgcccgg
     2221 caaactgcgg atcctgcatt ttcaccgaca gaacataggc gcagcgatcc cagatatctt
     2281 ccgccgacag cttcacgccg cgcggcagaa tgttgcggta ttcacagaac tcacgcatcg
     2341 cgtccaacag gccctgacgc agaccattaa catgggtacc gccctgcatc gttgggataa
     2401 ggttgacgta gctttcggtc agcagttcac cgccttccgg cagccacagt agcgcccagt
     2461 ccacagcttc agtatcgcca gcgaaattac cgataaacgg tttttctggc agcgtcggta
     2521 aaccgtttac cgcttccgcc aggtaatcat tcagaccgtc ctgatagcac cagcgctgtt
     2581 cggtgttgtt gatctcatct ttaaaggtaa tctcaacgcc ggggcacagt actgctttgg
     2641 ccttcagcac atgcgtcagg cgtgaaacag aaaaacgcgg gctgtcaaag aaggtttcat
     2701 ccggccagaa gtgcacgctg gtaccggtat tgcgtttacc gcaagtgccg acaacctgta
     2761 aatcctgcac cttttcgcca ttttcaaagg cgatgttata aacctgacca tcgcggcgca
     2821 cgttaacttc tacgcgcttc gacagggcgt taaccaccga aatccccacg ccatgcaggc
     2881 cgccagagaa ctggtaattt ttgttagaga atttaccgcc tgcatgcaga cggcaaagaa
     2941 tcagttcaac cgccggtaca ccctcttccg ggtgaatatc caccggcatc ccgcgcccat
     3001 cgtcaataac ttctaacgac tggtcagcat gtaagattac atccacgcgt tttgcgtgac
     3061 ccgccagtgc ttcatcgaca ctgttatcaa taacttcttg ccccaaatgg ttagggcgag
     3121 tggtatcggt atacatcccc ggacggcggc gaaccggctc aagcccggtg agtacctcaa
     3181 tggcatcagc gttataagtt tgcgtcatgg tttaagttag caattcgagt tgatcgtcag
     3241 agatggtgca gaccaagaaa atcgacgatc gggttgaaat aatcttcgaa gcccgtgaat
     3301 gcgtggttgc cgccttctat gacagtctgg cggcaggaag cgtagtacgc caccgcctgg
     3361 cggtaatcca gcacttcatc tcccgtctgt tgcagcagcc agatcaaatc cggcgcttcc
     3421 agcgggtcaa tctgcatgac tttgagatcg taaatatggc gtgactctag cacatattgc
     3481 tgcccggtgt aggggttctc gttctgaccg agatagtccg tcagcagttc aaacgggcgt
     3541 accgccgggt ttaccaccac tgcgggcagc ataaaacatt gcgacaacca ggtggcgtaa
     3601 tatcccccca gtgacgaacc gacaataccc agcgaatcgc cgccatgttc caggacaatg
     3661 gattccaaca gctctgccgc gtcggaagga tacggcggca actgcggaat gatcatctca
     3721 acgtcagggt gatgttccgc cagccagttt tttaacaagc tcgcttttgc agagcgcggc
     3781 gagctgttga aaccgtgtaa ataaagaagc gtagacatca gtagccttct gaagcggtat
     3841 caggttggaa acgtgtgtcc gccaggcgat gcacctcggt ggtcagcgtg ccatcagcat
     3901 gtaactcgag agtacgccag ccgggcgcga tggtgtccag cgtaaagttg gaacagtgcg
     3961 gcttaaactg cacacaggtc gacggcgttg ccagcaggcg gcgaccgttc cagtcgagat
     4021 ccagctcctg atgaatatga ccgcacagca agtatttgac gtgcggaaac ttcgccagca
     4081 cggtatccag ttcgcccgcg ttacgcagac tgtgttgatc gagccaacta caacccgcag
     4141 gtagcggatg atgatgcagc agcagcaacg tatggcgttc tggcgcatcg gccagtttac
     4201 gttccagcca ctcaagctga aactcgctca gctcaccgtg cggcacgcca aacacctggc
     4261 tatccagcaa caggatttgc cattgctcac caataaacac gcgcttcgcc ggggagatac
     4321 ccgcatcctg taacgcgctg tacatcgcgg gctggaaatc gtggttgccc ggcagccaga
     4381 cgcagggcgc acgaaaactt gcgatgcctt cagcgaaatg ctgataggcc gcagaggatt
     4441 gatcctgcgc taaatcacct gtcgcgacaa tcaggtcgaa ttcgtgctga tgtggacgaa
     4501 tcgcctccag caccgcctgg tagctttccc aggtgtttac ccctaacagg gcttcgtgct
     4561 tttgtgcaaa caggtgagta tcggtaattt gtaaaatcct gactctggcc tcaccagcca
     4621 gaggaagggt taacaggctt tccaaatggt gtccttaggt ttcacgacgc tagtaaaccg
     4681 gaatcgccat cgctccatgt gctaaacagt atcgcaacca gtccgctaaa aactggttaa
     4741 tttgatgctt ttcgtcgcgt tgatgcaact ttttattagg ataatcatac cgcgctttga
     4801 agcgaaaaat ctgctggctt gaacacactt cagccaccat cgcgtcatga tacagacgca
     4861 ccgtcattga cggaaggctc cagtaactga tcgcgggcgc agtctgttct attgtcacca
     4921 gggtagtgta tcgggtcgat tccacaatcg tcagccgata ttgtgcgttt gccacctgat
     4981 agcttacagt ttcgccgggt gcgtcattgc gcggtaacaa acggcgcaat tgtgaaaagt
     5041 tcatctcgca caggcgcatc atttcaggaa agtcaggtgt gtaacgcttc atttatgccc
     5101 actcattttt taacgcttga tgatgcagct gcagccattg caaagcgatg accgacgctg
     5161 cgttgtcgat tttcccctct tctacccact ggtatgcctg ttcccggctt accacatgaa
     5221 cgcgaatatc ttcgttttca tcagccagac catgaatacc gcttgcggtc gtggcgtcca
     5281 cttcgcccac cataattgac gaacgctcac tggtgccccc cgggcttgcc aggaaactta
     5341 acaccggttt ggtccgtttg actatcagtc ccgcctcttc aatcgcttcg cgacgggcaa
     5401 catcttccac actttcaccc tcttctatca tcccggcaac catctccagt agccaggggg
     5461 tttcactggt gtcgtatgcg gcaatccgaa tctgctcaat cagcacaact tcatcacgca
     5521 ctgggtcaaa gggtagcaag actgcggcgt gaccgcgctc aaaaatttcc ctccgtacct
     5581 catgactcat ttgcccgttg aataaacgat gacgaaatct ataaagatct aatgaaaaaa
     5641 agccgcgata aagtgtttct cgtgcaataa tttctacatc gtttttgcca aatgtaacgg
     5701 gcaggttgtc tggcttaagc attgttaatg tcctggcact aatagtgaat taaatgtgaa
     5761 tttcagcgac gtttgactgc cgtttgagca gtcatgtgtt aaattgaggc acattaacgc
     5821 cctatggcac gtaacgccaa ccttttgcgg tagcggcttc tgctagaatc cgcaataatt
     5881 ttacagtttg atcgcgctaa atactgcttc accacaagga atgcaaatga agaaattgct
     5941 ccccattctt atcggcctga gcctttctgg gttcagttcg ttgagccagg ccgagaacct
     6001 gatgcaagtt tatcagcaag cacgccttag taacccggaa ttgcgtaagt ctgccgccga
     6061 tcgtgatgct gcctttgaaa aaattaatga agcgcgcagt ccattactgc cacagctagg
     6121 tttaggtgca gattacacct atagcaacgg ctaccgcgac gcgaacggca tcaactctaa
     6181 cgcgaccagt gcgtccctgc agttaactca atccattttt gatatgtcga aatggcgtgc
     6241 gttaacgctg caggaaaaag cagcagggat tcaggacgtc acatatcaga ccgatcagca
     6301 aaccttgatc ctcaacaccg cgaccgctta ttttaacgtg ttgaatgcta ttgacgttct
     6361 ttcctataca caggcacaaa aagaagcgat ctaccgtcaa ttagatcaaa ccacccaacg
     6421 ttttaacgtg ggcctggtag cgataaccga cgtgcagaac gcccgcgcgc agtacgatac
     6481 cgtgctggcg aacgaagtga ccgcacgtaa taaccttgat aacgcggtag agcagctgcg
     6541 ccagatcacc ggtaactact atccagaact ggcagcgctg aatgtcgaaa actttaaaac
     6601 cgacaaacca cagccggtta acgcgctgct gaaagaagcc gaaaaacgca acctgtcgct
     6661 gttacaggca cgcttgagcc aggacctggc gcgcgagcaa attcgccagg cgcaggatgg
     6721 tcacttaccg acgctggatt taacggcttc taccgggatt tctgacacct cttatagcgg
     6781 ttcgaaaact cgtggtgccg ctggtaccca gtatgacgac agcaatatgg gccagaacaa
     6841 agtgggcctg agcttctcgc tgccgattta tcagggcgga atggttaact cgcaggtgaa
     6901 acaggcccag tacaactttg ttggtgccag cgagcaactg gaaagcgcgc atcgtagcgt
     6961 cgtgcaaacc gtacgttcct ccttcaacaa cattaatgca tctatcagta gcattaacgc
     7021 ctacaaacaa gccgtagttt ccgctcaaag ctcattagac gcgatggaag cgggctactc
     7081 ggtcggtacg cgtaccattg ttgatgtgtt ggatgcaacc accacgctgt acaacgctaa
     7141 gcaagagctg gcaaatgcgc gttataacta cctgattaat cagctgaata ttaagtcagc
     7201 cctgggtacg ttgaacgagc aggatctgct ggcactgaac aatgcgctga gcaaaccggt
     7261 ttccactaat ccggaaaacg ttgccccgca aacgccggaa cagaatgcta ttgctgatgg
     7321 ttatgcgcct gatagcccgg cacccgtcgt tcagcaaaca tccgcacgca ctaccaccag
     7381 taacggtcat aaccctttcc gtaactgatg acgacgacgg ggcttcggcc ccgtctgaac
     7441 gtaaggcaac gtaaagatac gggttatctg ccgcattctt cccccttctc gcttcaattt
     7501 cgaccagcca tcctctattc tgatgggtat ttaccactgg tcccggaaga caaaaatgaa
     7561 acggacaaaa tccatacgcc acgcatcgtt ccgcaaaaac tggagcgcgc gccatctgac
     7621 accagtcgct ctcgcggttg ccactgtttt tatgctggct ggctgtgaaa agagtgatga
     7681 aacagtgtct ctctatcaaa atgctgacga ctgttcagct gcaaacccag gcaaaagcgc
     7741 cgaatgtacc accgcgtaca acaatgcgct gaaagaagcc gaacgtactg cgccgaaata
     7801 cgccacccgt gaagactgtg ttgctgaatt tggtgaaggt cagtgccagc aggcaccagc
     7861 ccaggctggc atggcaccag aaaaccaggc gcaggcccag caatccagcg ggagtttctg
     7921 gatgccgctg atggccggtt acatgatggg gcgtctgatg ggcggcggcg cgggatttgc
     7981 acagcagccg ctgttctcct cgaaaaaccc agccagcccg gcttacggta aatataccga
     8041 cgcgacgggt aaaaactatg gcgcagccca gccaggccgc accatgaccg taccgaagac
     8101 agccatggca ccaaaaccgg cgaccaccac taccgttacc cgtggcggtt ttggtgaatc
     8161 tgttgccaaa caaagcacta tgcagcgtag cgcaactggc acctcttctc gttcaatggg
     8221 tggctgatac cgatggaaag agtcagtatt accgagcgcc cggactggcg tgagaaagcc
     8281 cacgaatacg gtttcaattt tcacaccatg tacggcgagc cgtactggtg tgaagatgct
     8341 tactacaagt tgaccctcgc ccaggttgaa aagctggaag aagtcaccgc cgaactgcac
     8401 cagatgtgcc tgaaagtggt ggaaaaagtg atcgccagcg atgagctgat gaccaaattc
     8461 cgcattccaa aacacacctg gagttttgtg cgccagtcat ggctgacgca ccagccatcg
     8521 ctttattcgc gtcttgatct ggcgtgggat ggcactggcg aacctaaact tctggaaaat
     8581 aacgccgata cgccaacgtc actatacgag gcggcgttct ttcagtggat ctggctggaa
     8641 gatcagctta acgccggtaa cttgccggag ggcagcgacc agtttaacag tctgcaagaa
     8701 aaactgatcg atcgcttcgt tgagctgcgt gaacagtatg gcttccagtt gctgcatctc
     8761 acctgctgtc gcgacacggt ggaagatcgc ggaaccattc agtatttgca ggactgcgca
     8821 acggaagctg aaattgctac tgagttcctc tacatcgatg atatcgggtt aggtgaaaaa
     8881 ggtcagttca cggatttaca ggatcaggta atttccaacc tgttcaagct gtatccgtgg
     8941 gaatttatgt tgcgtgagat gttttccacc aagctggagg atgcaggcgt acgctggctg
     9001 gaaccggcgt ggaagagcat tatctccaac aaggcacttc taccgctact gtgggagatg
     9061 ttcccgaatc acccgaacct gctgcccgct tattttgcgg aagatgatca tccgcaaatg
     9121 gaaaaatatg tggttaaacc gatcttctcc cgtgaaggcg caaacgtgtc gatcattgaa
     9181 aacggcaaaa ccattgaagc agcggaaggt ccgtatggcg aagaagggat gattgttcag
     9241 caattccacc cgttaccgaa attcggcgac agctatatgc tgattggtag ctggctggtg
     9301 aacgatcaac ccgccggaat tggcattcgt gaagaccgtg cattgatcac ccaggatatg
     9361 tctcggtttt atccacatat ttttgttgag taatcaatga tgccggatgg cattcgccat
     9421 ccggtaattg ttaacctatc tgcaccgaca gcatactcag gctgcccatt tctataccat
     9481 cgaccggaat ggtaattggc tcctgcccat cccacgcacc taagacatac aacaacggca
     9541 gatagtgctc cggcgttggg ttcgataacg cgccaccttc atggtcgagg taattcacca
     9601 gaggatgctg ctctaccgcc ccttgccacg tcagattcgc tttcacatac tcattaaacg
     9661 acatcgccca aggatatggt gaactatcac cgtgccactt cactgtacgc aggttatgca
     9721 ccacgttgcc actggcgacc aacattattc cttcatctcg cagcgctgcc agtttgcgcc
     9781 ccatttcgaa atgccaggcg gcaggtttgc tactgtcgat actcaactgc accatcggga
     9841 tatcggcgtc aggatacatc ttaatcagca cgccccagga accgtggtca aagccccagg
     9901 cttctttatc cagcgtcacc gggaccggcg ctaacagctc aaccagatgc tgtgccagca
     9961 caggcgaacc cggagcagga taatgcgtat cgtacagcgc ctgcgggaag ccaccaaagt
    10021 catgaatcgt gggcggcgtc tccatcgcgg tcactcctgt tccacgggta aaccagtgag
    10081 ccgaaaccac cacaatcgct tgcgggcgtg gcaatgtcat ccccaacttc tgccagctgc
    10141 gggtatacaa attatcttcc agcacgttca tcggactacc gtgacctaaa aacaatgctg
    10201 gcatacgtgt tgaagacatg atgatatcct taactaaagg tgtcattttg atatcctcac
    10261 aatacgcttg ttcggcggag taagaacccg gataacaatg atgatgatca tcagttattt
    10321 tgacgatctg cctgaaggtg aagatttata aggagttgtc gatgtcagta cctctcattc
    10381 tgaccatact ggcgggggca gccacgttta ttggcgcgtt tctcggcgtt ctcgggcaaa
    10441 aaccctcgaa ccgcttactg gcgttttcgc taggatttgc ggcggggatc atgttgctca
    10501 tctcattaat ggaaatgctt cctgccgcac tagcagctga aggaatgtcg cctgtgttgg
    10561 gttatggaat gtttatcttc ggtctgcttg gctattttgg cctggaccgc atgttgccac
    10621 atgcgcatcc gcaggattta atgcaaaaat cggtgcagcc gttgccaaaa tcgatcaagc
    10681 gcacagccat tctgctcact ctcggcatca gtctgcataa cttcccggaa gggattgcca
    10741 cctttgtcac ggcgagcagc aacctggagc tgggatttgg catcgcactg gccgtcgctt
    10801 tgcacaatat ccctgaaggt ctggcagtgg caggcccggt ttatgcggca acggggtcta
    10861 aacgcaccgc gattctgtgg gcggggattt ctggactagc agaaattctt ggtggtgtgc
    10921 tggcgtggtt aatcctcggt agcatgattt ccccggtggt catggcggca atcatggcgg
    10981 cggttgcagg aattatggtg gcgctctcgg ttgatgaatt aatgccgctc gccaaagaga
    11041 ttgaccctaa taataacccc agctatggcg tactatgtgg aatgtcagtg atgggattca
    11101 gtttagtgct gctacaaacg gcgggaattg gttaaaaaca acatcagaag ttgtccagca
    11161 catggacaac ttctgccagc ttcacgaaag ccttattcta ccttttcggc tgtctcttct
    11221 ctcgtactgt ttaagtattt gttccgcgct tcaagcagtg cgtctctgtc ctggcgaaac
    11281 gcccgacaat aacgtttcaa atgcaggaaa taacccacca caaccagagc aatcaataca
    11341 atccagtacc aggcgataaa catcctttta ccctta
//
LOCUS       DAPHDW010000140        11219 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.142, whole genome shotgun sequence.
ACCESSION   DAPHDW010000140 DAPHDW010000000
VERSION     DAPHDW010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11219
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.142"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            18..341
                     /locus_tag="RH057_003673"
     CDS             18..341
                     /locus_tag="RH057_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001442732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HDV3129987.1"
                     /translation="MVNFKDKNMPAVIDKALDFIGAMDVSAPTPSSMNESTAKGIFKY
                     LKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIKNPEYFSTYMQEE
                     LKALV"
     gene            complement(461..640)
                     /locus_tag="RH057_003674"
     CDS             complement(461..640)
                     /locus_tag="RH057_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HDV3129988.1"
                     /translation="MFAGLPSLTHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGERIVARFEDEDE"
     gene            714..2075
                     /gene="pabB"
                     /locus_tag="RH057_003675"
     CDS             714..2075
                     /gene="pabB"
                     /locus_tag="RH057_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HDV3129989.1"
                     /translation="MKTLSPAVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTFGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTREQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            2079..2657
                     /locus_tag="RH057_003676"
     CDS             2079..2657
                     /locus_tag="RH057_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HDV3129990.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINRETLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVKP"
     gene            2841..4205
                     /gene="sdaA"
                     /locus_tag="RH057_003677"
     CDS             2841..4205
                     /gene="sdaA"
                     /locus_tag="RH057_003677"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HDV3129991.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVEERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            4336..5934
                     /locus_tag="RH057_003678"
     CDS             4336..5934
                     /locus_tag="RH057_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416329.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3129992.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSEVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVMEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQRFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTR
                     YVMAETIRQRHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSANPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMPLR"
     gene            complement(5938..7494)
                     /gene="yoaE"
                     /locus_tag="RH057_003679"
     CDS             complement(5938..7494)
                     /gene="yoaE"
                     /locus_tag="RH057_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HDV3129993.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPMPI
                     PEGAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLF
                     PVCRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRAR
                     GSFVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGDGWLVKGGTDLHALQ
                     QALDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIIEANDYRVDLVRIV
                     KEQPAHDEDE"
     gene            7956..8927
                     /gene="manX"
                     /locus_tag="RH057_003680"
     CDS             7956..8927
                     /gene="manX"
                     /locus_tag="RH057_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HDV3129994.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            8990..9790
                     /gene="manY"
                     /locus_tag="RH057_003681"
     CDS             8990..9790
                     /gene="manY"
                     /locus_tag="RH057_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HDV3129995.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            9803..10654
                     /gene="manZ"
                     /locus_tag="RH057_003682"
     CDS             9803..10654
                     /gene="manZ"
                     /locus_tag="RH057_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HDV3129996.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVSR
                     ITDQTGKEHVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            10709..11167
                     /locus_tag="RH057_003683"
     CDS             10709..11167
                     /locus_tag="RH057_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 domain-containing protein"
                     /protein_id="HDV3129997.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSVIFVGLIVILIYNNVTNHGALITTWLLSALALMGFYIFWIRVPKIIFKQKGFFFA
                     NVWIEYSRIKAMNLSEDGVLVMQLEQRRLLIRVRNIDDLEKIYKLLVSTQ"
BASE COUNT         2738 a         2835 c         2844 g         2802 t
ORIGIN      
        1 atagtcttat cgataacatg gttaatttta aggataagaa tatgcctgct gtaatagata
       61 aagccctgga tttcattggt gccatggatg tatcagcgcc aacaccaagt tcgatgaatg
      121 aaagcacggc gaagggaatc tttaaatatt taaaagaact gggagtaccc gcgagtgccg
      181 ctgatattac ggcgcgagct gaccaggaag gctggaaccc tgggttcacg gaaaaaatgg
      241 ttggatgggc aaaaaaaatg gagacaggtg aacgctctgt gattaaaaat cctgaatact
      301 tttcaacata tatgcaggaa gaactaaaag cactggtctg agttaaattt atatcagcat
      361 aaatgggtca ggacgctttt aatcacatat aaaaagcgtc ctttttctca caatcaaatg
      421 atcatcggct tctataacga aatcgatacg gattagtgtg ttactcgtct tcatcctcaa
      481 aacgcgccac gattcgctcg ccggagtggt tggcacgcag ctcttctgcc accagcgcga
      541 ttgcctggcc gctgctcatc ccttgtgcca tcagttcctg gatccgctcg acagcttttt
      601 gctgctgttc atgggtgagt gaaggtaaac ctgcaaacat tgttaactcc tgctaaattg
      661 ttggcgctaa ttatttcatg ctacccggca catagccagt agagtcagga ctgatgaaga
      721 cgttatctcc cgctgtgatt actttaccct ggcgtcagga cgccgctgaa ttttatttct
      781 cccgcttaag ccacctgccg tgggcgatgc ttttacactc cggctatgcc gatcatccgt
      841 atagccgctt tgatattgtg gtcgccgatc cgatttgcac tttaaccact ttcggtaaag
      901 aaaccgttgt tagtgaaagc gaaaaacgca caacgaccac tgatgacccg ctacaggtgc
      961 tccagcaggt gctggatcgc gcagacattc gcccaacgca taacgaagat ttgccatttc
     1021 agggcggcgc actggggttg tttggctacg atctgggccg ccgttttgag tcactgccag
     1081 aaattgcgga acaagatatc gttctgccgg atatggcagt gggtatctac gattgggcgc
     1141 tcattgtcga ccaccagcgt catacagttt ctttgctgag tcataatgat gtcaatgccc
     1201 gtcgggcctg gctggaaagc cagcaattct cgccgcagga agatttcacg ctcacttccg
     1261 actggcaatc caatatgacc cgcgagcagt acggcgaaaa atttcgccag gtacaggaat
     1321 atctgcacag cggtgattgc tatcaggtga atctcgccca gcgttttcat gcgacctatt
     1381 ctggcgatga atggcaggca ttccttcagc ttaatcaggc caaccgcgcg ccatttagcg
     1441 cttttttacg tcttgaacag ggtgcaattt taagcctttc gccagagcgg tttattcttt
     1501 gtgataatag tgaaatccag acccgcccga ttaaaggcac gctaccacgc ctgcccgatc
     1561 ctcaggaaga tagcaaacaa gcagaaaaac tggcgaactc agcgaaagat cgtgccgaaa
     1621 atctgatgat tgtcgattta atgcgtaatg atatcggtcg tgttgccgta gccggttcgg
     1681 taaaagtacc agagctcttc gtggtggaac ccttccctgc cgtgcatcat ctggtcagca
     1741 ctataacggc gcgactacca gaacagttac acgccagcga tctgctgcgc gcagcttttc
     1801 ctggtggctc aataaccggg gctccgaaag tacgggctat ggaaattatc gacgaactgg
     1861 aaccgcagcg acgtaatgcc tggtgcggca gcattggcta tttgagcttt tgcggcaaca
     1921 tggataccag cattactatc cgcacgctga ctgccattaa cggacaaata tactgctctg
     1981 cgggcggtgg aattgtcgcc gatagccagg aagaagcgga atatcaggaa acttttgata
     2041 aagttaataa gatattacgc caactggaga agtaagacgt ggaataccgt agcctgacgc
     2101 ttgatgattt tttatctcgc tttcaacttt tgcgcccgca aattaaccgg gaaaccctaa
     2161 atcatcgtca ggctgccgtg ttaatcccca tcgtccgtcg accgcaaccg gggttgttgc
     2221 tgactcagcg ttcaattcat ctgcgtaaac acgctggaca agtggcattc cctggaggtg
     2281 cagtcgatga cacggacgca tcagttatcg ccgccgcgct gcgcgaagct gaagaagagg
     2341 tcgctatacc gccttccgcc gttgaagtta tcggcgtgct gccgcccgtc gatagcgtaa
     2401 ctggctatca ggtaacccca gtggtcggca ttatcccgcc cgatctgccg tatcgcgcca
     2461 gtgaagatga agtctcggcg gtgtttgaaa tgccgctcgc ccaggcatta catctgggtc
     2521 gttatcaccc tttagatatc taccgccgtg gcgattcaca tcgggtatgg ctgtcctggt
     2581 acgaacagta ttttgtatgg ggaatgaccg caggcataat tcgtgagctg gcgctgcaaa
     2641 ttggtgtgaa accctgacta tacttatctt tacatctaca aaacactact tgagacaatc
     2701 atcgcaatat tagttaaatc gcggtttttg attagtttaa ttcatgtgaa tagttaagcc
     2761 agtcgccgcg ttccctctta cactatgcgc tgttattagt tcgttactgg aagtccagtc
     2821 accttgtcag gagtattatc gtgattagtc tattcgacat gtttaaggtg gggattggtc
     2881 cctcatcttc ccataccgta gggcctatga aggcgggtaa acagttcgtc gatgatctgg
     2941 tcgaaaaagg cttactggat agcgttactc gcgttgccgt ggacgtttat ggttcactgt
     3001 cgctgacggg taaaggccac cacaccgata tcgccattat tatgggtctt gcaggtaacg
     3061 aacctgccac cgtggatatc gacagtattc ccggttttat tcgcgacgta gaagagcgcg
     3121 aacgtctgct gctggcacag ggacggcatg aagtggattt cccgcgcgac aacgggatgc
     3181 gttttcataa cggcaacctg ccgctgcatg aaaacggtat gcaaatccac gcctataacg
     3241 gcgatgaagt cgtctacagc aaaacttatt attccatcgg cggcggtttt atcgtcgatg
     3301 aagaacactt tggtcaggat gctgccaacg aagtgagcgt gccgtatccg ttcaaatctg
     3361 ccaccgaact gctcgcgtac tgtaatgaaa ccggctattc gctgtctggt ctcgctatgc
     3421 agaacgaact ggcgctgcac agcaagaaag agatcgacga gtatttcgcg catgtctggc
     3481 aaaccatgca ggcatgtatc gatcgcggga tgaacaccga aggtgtactg ccaggcccgc
     3541 tgcgcgtgcc acgtcgtgcg tctgccctgc gccggatgtt agtttccagc gataaactgt
     3601 ctaacgatcc gatgaatgtc attgactggg taaacatgtt tgcgctggca gttaacgaag
     3661 aaaacgccgc cggtggtcgt gtggtaactg cgccaaccaa cggtgcctgc ggtatcgttc
     3721 cggcagtgct ggcttactat gaccacttta ttgaatcggt cagcccggac atctataccc
     3781 gttactttat ggcagcgggc gcgattggtg cattgtataa aatgaacgcc tctatttccg
     3841 gtgcggaagt tggttgccag ggcgaagtgg gtgttgcctg ttcaatggct gctgcgggtc
     3901 ttgcagaact gctgggcggt agcccggaac aggtttgcgt ggcggcggaa attggcatgg
     3961 aacacaacct tggtttaacc tgcgacccgg ttgcagggca ggttcaggtg ccgtgcattg
     4021 agcgtaatgc cattgcctct gtgaaggcga ttaacgccgc acggatggct ctgcgccgca
     4081 ccagtgcacc gcgcgtctcg ctggataagg tcatcgaaac gatgtacgaa accggtaagg
     4141 acatgaacgc caaataccgc gaaacctcac gcggtggtct ggcaatcaaa gtccagtgtg
     4201 actaatactt cttactcgcc catctgcaac ggatgggcga atttataccc gctttctcgt
     4261 ctgctgtaat attccccact acacttccac tgttgcgtca ggcgtttgtc gccatacgct
     4321 tacagggtgg cccgcatgca aaaagcacaa cggatcatta aaacctatcg ccgtaatcga
     4381 atgattgttt gtacgatttg cgcactcgtt acgctcgctt cgaccctgag cgtgcgattt
     4441 atttcacagc gtaacttaaa tcaacaacgg gtagtacaat tcgccaatca cgctgtagag
     4501 gaattagata aagtactgct tcccctacag gcaggtagcg aagtcttgct tccgctgatt
     4561 ggtctgccct gctctgtcgc ccatttgcca ttacgtaaac aggcggcaaa actccaaact
     4621 gtgcgatcca ttggcctggt gcaagacggc acactctatt gctccagcat ttttggttat
     4681 cgcaatgtgc ccgtcgtgga cattctggct gaacttcctg caccgcaacc acttttacgc
     4741 ctgacgatcg accgtgccct gattaaaggc agtccggttt tgattcaatg gacgcctgca
     4801 gcgggcagta gcaatgctgg ggtcatggag atgattaaca tcgacttact gacggcaatg
     4861 ctccttgagc cacaactgcc gcaaatcagt agcgccagcc tgacggtgga caaacggcat
     4921 ttgctctatg gtaatgggct ggtagattcc cttccgcaac ctgaagacaa tgaaaactat
     4981 caggtttctt cgcaacgctt tccttttacc attaacgtta atggtccggg ggctacggcg
     5041 ctggcatggc actatcttcc aacacaatta ccgctggcgg tgctgctaag tttactggtg
     5101 ggctacatcg cctggctggc gaccgcttac cggatgagct tttcccgcga aatcaatctg
     5161 ggcctggcgc aacatgagtt cgaattgttc tgtcagcctt tgcttaatgc gcgcagccag
     5221 caatgtattg gtgtagagat tttgctgcgc tggaacaatc cgcgtcaggg ctggatttca
     5281 ccggatgtgt ttattcctat cgcggaagaa catcatttaa ttgtgccact gacccgctat
     5341 gtgatggcag aaaccattcg tcagcgccat gttttcccga tgagtagtca gtttcatgtt
     5401 ggcattaacg tcgcacccag ccattttcgc cgtggtgtgc tgataaaaga tctcaatcag
     5461 tactggttta gcgctaaccc gattcagcaa ctgatcctcg aaatcaccga acgcgatgcc
     5521 ttactggatg ttgattatcg gattgcccgc gagctacatc gtaaaaacgt caaactggcg
     5581 attgatgact tcggcaccgg caacagttca ttttcctggc ttgaaacatt acgtcctgac
     5641 gtgctgaaaa ttgataagtc atttaccgca gctataggtt ctgacgcggt taactcgacg
     5701 gtgaccgata tcatcatcgc gctggggcaa agactgaata ttgaactggt ggcggagggc
     5761 gtggaaacgc aagaacaggc gaagtatttg cgccgtcatg gcgtgcatat tttgcaaggg
     5821 tatttgtacg cacagccgat gccgctacgt gattttccca aatggctggc gggcagccaa
     5881 ccgccgcccg cccggcataa tggacatatc acgcccatta tgccgttacg ttaacgctta
     5941 ctcatcttca tcgtgcgccg gttgctcttt aacaatgcga accagatcaa cacgataatc
     6001 attggcttca atgatggtga tatgcagtgg ccctacatca atcacatcgc ccacacgggg
     6061 aatgtgacca tttgccgaga tcaccaggcc cgcgaccgtc gcgatatcat cgtcatcggc
     6121 aaggtgctca acatcaagcg cctgctgcaa ggcatgcaaa tctgtaccgc cttttaccag
     6181 ccagccgtca ccatcggtaa tgatttccgg cgtttcgtca gcgtccggga attcacccgc
     6241 aatggcttcc agcacatcca gcggcgtgac caagccttgt accacaccaa actcgttggt
     6301 cacgataaca aagctcccgc gagcgcgacg cagcacgccc agcaggttga tcggatcgag
     6361 ggtttccggg acgataatcg ccggagacgc cgaagcaatc gccgccacat caacgccctc
     6421 ttccagcgcc accagcagtt ctttagcacg tacgattccg atgatttcat ccagttcacc
     6481 gcgacatacc gggaacaggc tgtgcggtga ggagagcagc tgctcgcgga tttcatcgac
     6541 cccgagatta gcgtcaaccc agcttatttc accgcgcggc gtcatgatcc cgcgcagaga
     6601 acgcgacgcc agcgtcagta cgccgttaat catgtaacgt tcttcttcgg caaatgcacc
     6661 ttccgggatc ggcatcggca tcgggttatc ggcatcgtgc tgaacattgg cctgacgttt
     6721 cccgcccatc aaacgcagga tggcatcggc agtacgcgct cgcagcggca aagtcgactg
     6781 gtggcgaata aagttgcgac gcgcaatctg gttaaacact tcgatgatga tcgagaagcc
     6841 aatcgcggca tacaggtaac ctttcggaat gtggaaaccg aaaccttctg ccaccagact
     6901 cagaccaatc attaacagga agctcagaca gagcaccacc accgtggggt gctggttaac
     6961 gaatcgcgtc agcggtttgg atgccagcaa cataaccgcc atcgcaatca ctaccgccgc
     7021 catcatcacc ggcagatggt taaccatccc tactgcagta attaccgcat ccaacgagaa
     7081 gacggcgtca aggatgacaa tctgtgtgac gaccacccag aaactggcgt agcctttacc
     7141 gtggccggaa tcatgatcgc ggttttccag ccgttcatgc agttcggttg ttgctttgaa
     7201 cagcaagaat atccccccga acaacataat caggtcgcgt ccggagaagg agaaatccat
     7261 gacggtaaat agcggtttgg tcagcgtgac catccatgaa atcagcgaca gcagccccag
     7321 acgcataatc agcgccagtg ataaccccag caaacgcgct ttatcgcgtt gttttggcgg
     7381 cagtttgtca gcaagaatgg cgatgaagac caggttatcg atacccagca caatttcgag
     7441 aacaacaagc gtgagtagcc ccgcccaaat tgaggggtcc attaagaatt ccatgacaag
     7501 ctcctgctta aggaatagct attcgacgcc agaaataatg caggcgtaac gacaaaatgc
     7561 aaacgaaagg tgcggcatag agagccagaa aggtaggcgt taaaaggcct gatgctgaaa
     7621 tgacgtcggt gacgatccat actgcgggct actgccctat actccatggt tgttaaacgg
     7681 gagttaaaca tatcagagac gcctctgatt tggcaaagat ttaccttcct ttgcaaacga
     7741 atgtgacaag gatattttac ctttcgaaat ttctgctaat cgaaagttaa attacggatc
     7801 ttcatcacat aaaataattt tttcgatatc taaaataaat cgcgaaacgc aggggttttt
     7861 ggttgtagcc cttatctgaa tcgattcgat tgtggacgac gattcaaaat acatctggca
     7921 cgttgaggtg ttaacgataa taaaggaggt agcaagtgac cattgctatt gttataggca
     7981 cacatggttg ggctgcagag cagttgctta aaacggcaga aatgctgtta ggcgagcagg
     8041 aaaacgtcgg ctggatcgat ttcgttccag gtgaaaatgc cgaaacgctg attgaaaagt
     8101 acaacgctca gttggcaaaa ctcgacacca ctaaaggcgt gctgtttctc gttgatacat
     8161 ggggaggcag cccgttcaat gctgccagcc gcattgtcgt cgacaaagag cattatgaag
     8221 tcattgcagg cgttaacatt ccaatgctcg tggaaacgtt aatggcccgt gatgatgacc
     8281 caagctttga tgaactggtg gcactggcag tagaaacagg ccgtgaaggc gtgaaagctc
     8341 tgaaagccaa accggttgaa aaagccgcgc cagcacccgc tgccgcagca ccaaaagcgg
     8401 ctccaactcc ggcaaaacca atggggccaa acgactacat ggttattggc cttgcgcgta
     8461 tcgacgaccg tctgattcac ggtcaggtcg ccacccgctg gaccaaagaa accaatgtct
     8521 cccgtattat tgttgttagt gatgaagtgg ctgcggatac cgttcgtaag acactgctca
     8581 cccaggttgc acctccgggc gtaacagcac acgtagttga tgttgccaaa atgattcgcg
     8641 tctacaacaa cccgaaatat gctggcgaac gcgtaatgct gttatttacc aacccaacag
     8701 atgtagagcg tctcgttgaa ggcggcgtga aaatcacctc tgttaacgtc ggtggtatgg
     8761 cattccgtca gggtaaaacc caggtgaata acgcggtttc ggttgatgaa aaagatatcg
     8821 aggcgttcaa gaaactgaat gcgcgcggta ttgagctgga agtccgtaag gtttccaccg
     8881 atccgaaact gaaaatgatg gatctgatca gcaaaatcga taagtaacgt attgtgttga
     8941 ttatcactca gttttcacac ttaagtctta cgtaaacagg agaagtacaa tggagattac
     9001 cactcttcaa attgtgctgg tatttatcgt agcctgtatc gcaggtatgg gatcaatcct
     9061 cgatgaattt cagtttcacc gtccgctaat cgcgtgtacc ctggtgggta tcgttcttgg
     9121 ggatatgaaa accggtatta ttatcggtgg tacgctggaa atgatcgcgc tgggctggat
     9181 gaacatcggt gctgcagttg cgcctgacgc cgctctggct tctatcattt ctaccattct
     9241 ggttatcgca ggtcatcaga gcattggtgc aggtatcgca ctggcaatcc ctctggccgc
     9301 tgcgggccag gtactgacca tcatcgttcg tactattacc gttgctttcc agcacgctgc
     9361 ggataaggct gctgataacg gcaacctgac agcgatttcc tggatccacg tttcttctct
     9421 gttcctgcaa gcaatgcgtg tggctattcc ggccgtcatc gttgcgctgt ctgttggtac
     9481 cagcgaagta cagaacatgc tgaatgcgat tccggaagtg gtgaccaatg gtctgaatat
     9541 cgccggtggc atgatcgtgg tggttggtta tgcgatggtt atcaacatga tgcgtgctgg
     9601 ctacctgatg ccgttcttct acctcggctt cgtaaccgca gcattcacca actttaacct
     9661 ggttgctctg ggtgtgattg gtactgttat ggcagtgctc tacatccaac ttagcccgaa
     9721 atacaaccgc gtagccggtg cgcctgctca ggcagctggt aacaacgatc tcgataacga
     9781 actggactaa caggtgagcg aaatggttga tacaactcaa actaccaccg agaaaaaact
     9841 cactcaaagt gatattcgtg gcgtcttcct gcgttctaac ctcttccagg gttcatggaa
     9901 cttcgaacgt atgcaggcac tgggtttctg cttctctatg gtaccggcaa ttcgtcgcct
     9961 ctaccctgag aacaacgaag ctcgtaaaca agctattcgc cgtcacctgg agttctttaa
    10021 cacccagccg ttcgtggctg cgccgattct cggcgtaacc ctggcgctgg aagaacagcg
    10081 tgctaatggc gcagagatcg acgacggtgc tatcaacggt atcaaagtcg gtttgatggg
    10141 gccgctggct ggtgtaggcg acccgatctt ctggggaacc gtacgtccgg tatttgcagc
    10201 actgggtgcc ggtatcgcga tgagcggcag cctgttaggt ccgctgctgt tcttcatcct
    10261 gtttaacctg gtgcgtctgg caacccgtta ctacggcgta gcgtatggtt actccaaagg
    10321 tatcgatatc gttaaagata tgggtggtgg cttcctgcaa aaactgacgg aaggggcgtc
    10381 tatcctcggc ctgtttgtca tgggggcatt ggttaacaag tggacacatg tcaacatccc
    10441 gctggttgtc tctcgcatta ctgaccagac gggcaaagaa cacgttacta ctgtccagac
    10501 tattctggac cagttaatgc caggcctggt accactgctg ctgacctttg cttgtatgtg
    10561 gctactgcgc aaaaaagtta acccgctgtg gatcatcgtt ggcttcttcg tcatcggtat
    10621 cgctggttac gcttgcggcc tgctgggact gtaagactgt tgtacactac cggggccttt
    10681 tggccccgtt tttttatctg gaggattaat gacaatcacg gacctggtac tgattctttt
    10741 catcgccgca ctcctggcct tcgcgatcta cgatcagttc atcatgcccc gccgtaacgg
    10801 ccccaccctg ctggcaattc ctttgctccg gcgtggtcgc atcgatagcg ttatcttcgt
    10861 cggattgatt gtcattctta tctataacaa cgtcacgaat catggtgcgt taataacgac
    10921 atggttatta agcgcactgg ctctgatggg tttttatata ttctggatcc gcgttccgaa
    10981 gatcatcttt aaacaaaaag gttttttctt cgccaatgtc tggattgaat atagccgaat
    11041 caaagcgatg aacttgtcgg aagatggcgt gttggtgatg caattagaac agcgtcggct
    11101 gttaatccgc gttcgaaata tcgacgatct ggaaaaaatt tataagcttc tcgtttcaac
    11161 tcaataagtt atgaatttag ccaaagctat gtttagtgta tttttaataa tcagacata
//
LOCUS       DAPHDW010000141        11120 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.143, whole genome shotgun sequence.
ACCESSION   DAPHDW010000141 DAPHDW010000000
VERSION     DAPHDW010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11120
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.143"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            22..222
                     /locus_tag="RH057_003684"
                     /pseudo
     CDS             22..222
                     /locus_tag="RH057_003684"
                     /inference="COORDINATES: protein motif:HMM:NF025054.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            660..806
                     /locus_tag="RH057_003685"
     CDS             660..806
                     /locus_tag="RH057_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001394772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3129998.1"
                     /translation="MVDLFGCTGAIAGQVPAVGSEVLSRVIASGLKVLTRVLVAGDEV
                     LPRE"
     gene            complement(941..2541)
                     /locus_tag="RH057_003686"
                     /pseudo
     CDS             complement(941..2541)
                     /locus_tag="RH057_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(2548..2940)
                     /gene="tnpB"
                     /locus_tag="RH057_003687"
                     /pseudo
     CDS             complement(2548..2940)
                     /gene="tnpB"
                     /locus_tag="RH057_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950651.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(2927..3256)
                     /locus_tag="RH057_003688"
     CDS             complement(2927..3256)
                     /locus_tag="RH057_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001325657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV3129999.1"
                     /translation="MSKPRWTIEQKRQHVAAWRASGLTRQQYCELNDIPFTSLREWPQ
                     DVAKAERRANEPTVLPVHIAPPLHADVPQPVTNEPVTLLLPGGVRMCCQPSQLTDVFR
                     ALKYAQS"
     gene            complement(3256..3802)
                     /locus_tag="RH057_003689"
                     /pseudo
     CDS             complement(3256..3802)
                     /locus_tag="RH057_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522305.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            complement(3805..4082)
                     /locus_tag="RH057_003690"
                     /pseudo
     CDS             complement(3805..4082)
                     /locus_tag="RH057_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_046881606.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(4443..5351)
                     /locus_tag="RH057_003691"
     CDS             complement(4443..5351)
                     /locus_tag="RH057_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3130000.1"
                     /translation="MANWAQKLKLHHLEMLVALGEQGNLTHVARMMNITQPALSKWLS
                     QLEDEIGITLFERHSKGLRPTEGGKLLLQHAQRLINDMERSQHELARFKQGGLVGSLK
                     IGCSPVATDCVSQAILHLLTKMPTLHLNIEEKVMTPLLQDLLAGSVDVVVGRIGGRAL
                     QLPLNYQVLYTEPVCFVARPEHPLAKYATLSWNDLANWRWIVWPTGTPIRISIDNALV
                     DHGVMLPENTIESASMNVSTNLLQSSDMISILSLRLAQRYASQGQLVILNLPKIEQKG
                     SVGVFWRNNETPSYALSQFLHFLQQN"
     gene            5478..6836
                     /locus_tag="RH057_003692"
     CDS             5478..6836
                     /locus_tag="RH057_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid/H+ symport family MFS transporter"
                     /protein_id="HDV3130001.1"
                     /translation="MTQRRDLQALIDAAPVGKMQWRVIICCFLVVMLDGFDTAAIGFI
                     APDIRTHWQLTAGDLAPLFGAGLLGLTAGALLCGPLSDRFGRKRVIELCVFLFGALSL
                     ASAFSPDLQTLVFLRFLTGLGLGGAMPNTITMTSEYLPARRRGALVTLMFCGFTLGSA
                     FGGIVSAQLVPVIGWHGILVLGGVLPLMLFVALLVVLPESPRWQVRRQLPQAVIAKTV
                     SAITRERYVDTHFYLIESASVTKGSIRQLFMGRQLPITLMLWVVFFMSLLIIYLLSSW
                     MPTLLNHRGIDLQHASWVTAAFQIGGTLGALALGVLMDKFNPFRVLALSYATGAICIV
                     MIGLSQDGLWLMALAIFGTGIGISGSQVGLNALTATLYPTQSRATGVSWSNAIGRCGA
                     IVGSLSGGVMMAMNFSFDTLFFIIAVPAAISAVMLTLLITVVRQSTSVPDSLPRAGVV
                     NE"
     gene            6848..7876
                     /gene="gtdA"
                     /locus_tag="RH057_003693"
     CDS             6848..7876
                     /gene="gtdA"
                     /locus_tag="RH057_003693"
                     /EC_number="1.13.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gentisate 1,2-dioxygenase"
                     /protein_id="HDV3130002.1"
                     /translation="MTDNNQNSREQFYQHISGQNLTPLWESLHHLVPKTPNANCAPAY
                     WNYQEIRPLLLESGSLIGAKEAVRRVLVLENPALRGQSSITATLYAGLQLIMPGEVAP
                     SHRHNQSALRFIVEGKGAFTAVDGERTPMNEGDFILTPQWRWHDHGNPGDEPVIWLDG
                     LDLPLVNTLGCGFAEDYPEDQQPVTRKEGDYLPRYAANMLPLRHQTGNSSPIFNYRYD
                     RSREVLHDLTRMGDADEWDGYKMRYVNPVTGGYPMPSMGAFLQLLPKGFSSRVARTTD
                     STIYHVVEGSGQVTIGNEIFTFSAKDIFVVPTWHGVSFQTTQDTVLFSFSDRPVQEAL
                     GLFREARY"
     gene            7892..8593
                     /locus_tag="RH057_003694"
     CDS             7892..8593
                     /locus_tag="RH057_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV3130003.1"
                     /translation="MTQYVFEPQAPVTVPVVGSDKQFPVRRVYCVGRNYAAHAREMGF
                     DPDREPPFFFCKPADAVIPVAAGETLALPYPAQTDNYHYEIELVVAIGKKGSDIPLEH
                     AHEYVWGYATGLDMTRRDRQMEMRQMGRPWEIGKAFDLSAPIAPLHKARDIGGIDNAP
                     IWLQVNGEDHQRSDIRHLIWSVNETISYLSGFFELQPGDLIFTGTPEGVGPVVKGDVI
                     TGNVEGLTPIAVKIV"
     gene            8602..9246
                     /gene="maiA"
                     /locus_tag="RH057_003695"
     CDS             8602..9246
                     /gene="maiA"
                     /locus_tag="RH057_003695"
                     /EC_number="5.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maleylacetoacetate isomerase"
                     /protein_id="HDV3130004.1"
                     /translation="MKLYSFFNSSASYRVRIALALKGINYQTEGVNIRIGQQNELAYR
                     RMNPVGLVPTLLTDEGESLGQSLAIIDWLERHYPQVPLVPQEEPARNKVLEIVYAIAC
                     DIHPLNNLRVLRYLTEELNVSEEEKKRWYAHWIQQGLSAVEQLLRQNQSGQFCVGETP
                     TLADCCLVPQWANALRMNCDLSGYPRCKAVYDACTQLPAFIAAAPENQQDKISA"
     gene            9261..10454
                     /locus_tag="RH057_003696"
     CDS             9261..10454
                     /locus_tag="RH057_003696"
                     /EC_number="1.14.13.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybenzoate 6-monooxygenase"
                     /protein_id="HDV3130005.1"
                     /translation="MTKVTRAVIVGGGIGGAATALSLARLGIKVVLLEKAHEIGEIGA
                     GIQLGPNAFSALDSLGVGEVARQRAVFTDHITMMDAVNAEEVVRIETGQAFRDHFGGP
                     YAVIHRVDIHASVWEAVLTHPDVEYHTSTNVVDIRETEDDVTVFDDRGNSWTGDILIG
                     CDGVKSVVRQSLLGDAPRVTGHVVYRAVIDCADMPEDLRINAPVLWAGPHCHLVHYPL
                     RGGTQYNLVVTFHSRQQEEWGVKDGSKEEVLSYFEGIHPRPRQMLDKPTSWRRWSTAD
                     REPVAKWGTNRITLVGDAAHPVAQYMAQGACMALEDAVTLGKALAQCEGDAAQAFALY
                     ESVRIPRTARIVWSTREMGRIYHAAGVERQVRNLLWKGKSQAEFYRGMEWLYGWKEDN
                     CLQPR"
     gene            10804..10944
                     /locus_tag="RH057_003697"
     CDS             10804..10944
                     /locus_tag="RH057_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001645505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130006.1"
                     /translation="MPPHKIRQEWLALDETAEVILQHSITLQSADLIRHSFIWRNRSI
                     CL"
BASE COUNT         2540 a         2899 c         2963 g         2718 t
ORIGIN      
        1 aaatgatggt gaaaatataa acttgctgat ataataatta atgaaggtta cagggggttt
       61 ggagtaggga gcgttctttt aacaaaagcg atagaatcag catcatcact cggggtaagg
      121 aaaatttacg gtgtcatgtg cggagatact aaaagattaa tcaaattcta caaatccttt
      181 gggtttgaaa ttcacggtaa tagtatagag ctccatctat aaatagataa cacaagaact
      241 ctatccaaac ataataatcc cctgaggcag gcagcagtga tcactgagag cattgcatgg
      301 aagcgccagt gataccccta cgcagtatcc tgacgccctg agcgactatc ccgtcctctg
      361 ttctctcatc cctgacgctg aaacccggca tcactactgc ctcagtgctt gctggcctga
      421 ccttattggc ccgtaatcac cgtaagccgg gaactggcgt tgtacgagtg gcacttgcca
      481 tcaatgcgag ttcaccgcaa cgttacgaat ttggttaatt attcatcctg caattgctaa
      541 ttgttgagaa aaccagctct gtatcgctca agtgtaaggt aagcttccgg gcatctcatc
      601 ttgacgtgtt tttcgggcgc tcgtcatcgg cggcgcatag gaacgatcgt gctctggcga
      661 tggttgattt atttggttgc acaggggcga tcgccgggca ggtaccggcg gtgggctctg
      721 aagtactgag tcgggtaatt gcgtcaggtc tcaaagtact gacacgggta ctggtggcag
      781 gtgatgaagt actgccccgg gaatgactcc gggctgattc aggttcttat tcgctgtcag
      841 cccgcgtggt tgctgccctg acgcatttcc tgtcgacagg cgttcaactc ccgactgcag
      901 gttatagccc gatgcacaaa cacctgacgg caggtatcac tcacccggag aaggtaaagc
      961 cctcaagagg tagcaactct gccagtcggt cctccggcca ctccggcaga cgcgtcagga
     1021 cgtctgtcag ccaggcatgt ggctccagac cgttgcgttt cgcggtttcc agcaagctca
     1081 ttatttgcgc ggcgcgttct cccgccatct gcgaaccggc gaacagccac gattttctgc
     1141 ccagagccac gtttttgatg gcccgttcac acacattatt atccagcggc accgcaccat
     1201 cttccaggaa gcagctcagt tccacgcgat gagacagcgc atagacaatg gctttgtgta
     1261 atgcccttcc cggaaaacat tgcggttcct gctcttcaag ccatgaccac agttcttcca
     1321 gtatcggacg ggcataacgc tgtcgccact ggcggatttt ttccacgggg cggctactga
     1381 tcttcttctc aagacggtac aaccccgcga ttttcttcat ggctatggca gcccgtggat
     1441 ctttactgat tttatacagg tcggcgaagc ccctgcgggc atggcccagc atccggccag
     1501 cgtgatctcc ggcactttgt ttgccagagt ccggtaagct ttatgtccgt cgacaaccag
     1561 cgtcccgccc cagccctgaa gccagttttc aggatactca tggctacgtc cggtccggca
     1621 gttgaagccc acaactgacg gtcccgtcct ttctccactg acgtatgccc acagataacc
     1681 ggagcaggat ttaccgcctt tcttcgtgtt caggagcttc agggtagtct catctgcatg
     1741 caccaccgga cggtttatca actcgcgatg gagtaacgcc gccaggggag ataatgcggc
     1801 acccgccgcg ccaaccatgt cagccatcga actgacggga agcccgacat ctgaacgggc
     1861 aaagacctgt tgctggcgat acagaggcag gtggtcaccg tatttattga tgatcacctg
     1921 tgcgatgact gatggctcaa cggcactttt cgggaggata tgtgccggca tttcaccgct
     1981 gaacaccttc tggcaacagg gacaactgta ttgcggacgg acataacagt tcaccacgaa
     2041 gtgagcggga atatactcca gcttttcact caccgcatca cggatatagt gcagcggctc
     2101 gtcacattca gggcaatggt cagtgtccgg ctggataatt ttttccaccc gtggaagatg
     2161 agccggtaag gatttgcgta tcgggcgtga acgggacgct ttttcgtctt cttcagggga
     2221 ttgcgggagc agtttatcca gatgtgcggt aagcgcggcg atatcgacat caacatcctc
     2281 ttcgaacagg gagcgttgca taccagccag cgtttcacat tttttaccga agcgctgttg
     2341 acgaaccagt ttcagcattt cttccagaag ctggatgcgc cggcttttct ccagtaattc
     2401 ccgctctttt tcggcattct ccgacatgac tttttgtacc atggcaagtg ccatggcacg
     2461 cagtttttcg atgtcattcg tggtgttgag agcggagatg tccatacagg ggagcatacc
     2521 actccggata cggatattaa ttatttttca attagttagc agtgtatttt gcgtgttttc
     2581 agggcgcagt tcaggttcat tctgccagac ccatttcgtc aggtcatgtc ccttaacctg
     2641 ctgccagtca acaccggcaa tcagccagtt aaactcgtcg ggagtgaggt gctaggcagc
     2701 gtcatttgca catggccagc ggaagctgcc tttgtgcaga cggcgggtac acaaccacac
     2761 cccgtgttta tcccaccgga gaactttgat acgcgaacgg gctttgttaa caaagacgaa
     2821 ggcggctccc ccatgccata tcgaccggag ttcatcctgt atatactgcg tcagtgcgtc
     2881 gatgccccgg cgcatgtcaa caggtttaat tgcgatgaag acattatcag gactgagcat
     2941 atttgagcgc cctgaagaca tcggtaagtt gtgacggctg acagcacatc cgtactccac
     3001 cgggaaggag gagcgtgact ggctcattcg tgacgggctg tggtacatca gcatgcagtg
     3061 gtggcgcaat atgaacggga agaacggtcg gttcatttgc ccggcgttca gcttttgcga
     3121 catcctgcgg ccattcgcga agtgatgtga agggaatatc gttgagttca cagtattgct
     3181 gacgtgtaag accactggcg cgccaggcag caacgtgctg ccttttttgt tcaatggtcc
     3241 atcggggttt tgacatcaat tatctccggt aagttgatga atgaatgtcg gtaatgctaa
     3301 ctgactgaaa atcgctgtga agatgacttc cccgtaacga cacagaatat cttccgcaat
     3361 acgctgacta ccagaccaga acagaccgtc cagtgcggtc accagtaatc cctcctctag
     3421 cgcatactta agtcattcac ggagcgcttt cactttccgg gcaatctgct gccaaggcga
     3481 tgatgtattt cattcccaac catccggcga atacggtgag acagatgata ccggaatgta
     3541 tccgcacgcc ggaaagactg gccttcaggc tatacacgca accaaagcgt ttatcgttaa
     3601 acaccacatt tttctggctg atatcccatt cttcccaaag cacagcaatc agctgtgacg
     3661 tgcgggttaa aagcagacga aaaggcagtt caaaactggt ggtataatcc acattcagca
     3721 gggcattacg cagccgctct cctgatccga cctctgcctg ccgacactcc tcctgaacat
     3781 cctgccactg ctggcgaagg cgtacaacaa cacgaacatt ctcagcgtat ttttttgagt
     3841 aaccaactga aaaatggtgg gaagatcatt tttcagttga gaattcccca acggtcaatc
     3901 cacccgcttt atcttgtttt taactaatgc tgtctggtta aatgacgccc gatgctgcta
     3961 agcgtgaggg aagcgccatt tattacagca tttgttgcgt caatcagtga tttttgatgg
     4021 tagaaatgca aagtggccag tatgttttta aggaattttt ggcataatga acgtgcaggc
     4081 atagtggtga tatccttgaa attgctagca caatcaatta gatcacatct cactatgcct
     4141 gtcttgtttt ctgggaattc ctaatgggtt gtccttccaa ttggttcttt cgaggcctgc
     4201 tcagattcac tcacattata acctgtatac tcactgaaac acctacagct tcttcatcaa
     4261 atacatcagc cgctttgtta cccacacgac tgctttgact gctaccgcct ggaacgacag
     4321 tcacctggtg ggattttcac cccatgaaag atcacgcctt atcacagtgc acgattcccc
     4381 gaaaaattac gcatcgttga attacaggga agtaacatcg tgcgaatgcc cagttcagca
     4441 cactaattct gctgtaaaaa gtgcagaaac tgacttaaag cataagaagg cgtttcgtta
     4501 ttgcgccaga acacaccgac gctgcctttc tgttctatct ttggcaggtt gagtatcacc
     4561 agttgtccct ggctggcata acgttgcgcc aggcgtaatg acaaaataga gatcatatcg
     4621 ctgctttgta acaggttggt actgacgttc atcgacgccg actcaatcgt gttttctggc
     4681 agcattacgc cgtggtctac cagcgcatta tcaatactaa tgcgaatggg ggtacccgtc
     4741 ggccagacga tccagcgcca gttggcaaga tcattccagc tgagggtggc gtatttcgcc
     4801 agtgggtgct cagggcgggc gacaaagcat actggctcgg tgtacagcac ctgataattc
     4861 agtggcagtt gcaaggcgcg accgccaatt cgcccaacga ccacgtcgac agaacctgcc
     4921 aacagatctt gcagcaatgg ggtcatcacc ttctcttcga tattcaggtg tagagttggc
     4981 atcttcgtaa gcaaatgcaa aatcgcctgg gatacgcagt cagtggcgac gggagaacag
     5041 ccaattttca gactgccgac cagtccacct tgtttaaaac gggccagttc atgttgcgat
     5101 cgctccatgt cattgattaa ccgttgggca tgttgcaata acaatttgcc gccttcagtc
     5161 ggacgtaatc ctttgctgtg ccgctcaaaa agtgtgattc caatctcatc ttcgagttgt
     5221 gacaaccatt tcgaaagcgc aggttgggtg atattcatca ttcgtgcgac atgggtcaga
     5281 ttgccctgtt cacccagcgc caccagcatt tcaaggtgat gtagttttaa tttttgcgcc
     5341 cagtttgcca tttaccataa cctccaggtt atctctctgc ccgaaatgcc attgtacatt
     5401 tcatctcttt aatcacaata ttgcaacata tacaaaacaa aagaaacatc taccttacct
     5461 gcactgaggc atttactatg actcaacgac gtgatctaca agcacttatc gatgctgctc
     5521 cggtcggcaa gatgcagtgg cgcgttatca tctgctgttt tctggtggta atgctcgatg
     5581 gttttgacac cgcggctatc ggatttatcg cccctgatat tcgcactcac tggcaactca
     5641 ccgcaggcga tctcgccccg ctttttggcg cgggtctgct ggggttaacc gcaggcgcac
     5701 tgctttgtgg cccgttgtcc gatcgctttg gtcgcaagcg ggtgattgag ctgtgcgtct
     5761 ttttgtttgg cgcgttaagc ctcgcctcag ctttctctcc ggatctgcaa actctggtct
     5821 tcctgcgctt tctcaccggc ctgggtctgg gcggcgcgat gccgaacacc atcaccatga
     5881 cctcggagta tctgcctgcc cgccgccgcg gcgcgctggt gacgctgatg ttttgcggct
     5941 ttacgctggg ttcggcgttt ggcgggattg tcagtgcgca actggtgccg gtgattggct
     6001 ggcacggcat tctggtgttg gggggcgtgt taccgctgat gctgtttgtc gcactgctgg
     6061 tagttctgcc ggaatcgcca cgctggcagg tgcgccgcca gctaccgcag gcggtgattg
     6121 cgaaaaccgt cagcgccatc acccgcgagc gttatgtcga tacccatttt tatctcattg
     6181 aatcagcctc cgttacaaaa ggcagcattc gtcaactgtt tatgggacgt cagttaccca
     6241 tcacgttgat gctgtgggta gtgttcttta tgagcctgct gattatttac ctgctctcaa
     6301 gctggatgcc gacgctactg aaccatcgcg gcatcgacct gcaacacgcg tcctgggtca
     6361 ccgccgcgtt ccagattggt ggtacgttag gcgcgctggc gctgggtgtg ctgatggata
     6421 agttcaatcc tttccgcgta ctggcgctga gctatgccac aggcgctata tgcatcgtga
     6481 tgattggctt aagccaggat ggattatggc tgatggcgct ggcgattttc ggcactggca
     6541 ttggcattag cggatcgcag gtggggctga atgcgctgac cgcaacactt tatccaacac
     6601 aaagtcgcgc tacgggcgtg agctggtcaa atgctatcgg ccgctgcggc gcgattgttg
     6661 gttcgctgtc tggcggcgtg atgatggcga tgaatttctc tttcgacacc ctgtttttca
     6721 ttatcgctgt gcctgcggcg atcagtgcgg tgatgttaac cctgctgatt accgtggttc
     6781 gccagtcgac ctctgtccct gactcactgc ctcgcgcagg cgttgtgaac gaataaggag
     6841 catcccaatg actgataaca atcaaaatag ccgcgagcaa ttttatcagc atatttccgg
     6901 gcaaaacctg accccgctgt gggagtcact gcaccatctg gtgccgaaaa cgcccaacgc
     6961 taactgtgcg ccagcgtact ggaattatca ggagatccgc ccgctgctgc tggagagcgg
     7021 cagcctgatt ggcgcgaaag aggcggtgcg ccgcgtgctg gtactggaaa acccggcgct
     7081 gcgagggcag tcttccatca ccgcgacttt atatgccggt ttgcaactga tcatgccggg
     7141 cgaagtggca ccgagtcatc gtcataacca gtcggcgctg cgttttattg ttgaaggcaa
     7201 aggggcattt accgccgttg acggcgaacg cacgccaatg aatgagggcg attttatcct
     7261 gaccccgcag tggcgctggc acgatcacgg caaccctggc gacgaaccgg ttatctggct
     7321 cgatgggttg gatctgccat tagtgaacac tctgggctgc ggttttgccg aagattaccc
     7381 ggaagatcaa caaccggtaa cgcgtaaaga gggcgattac ctgccgcgtt acgctgccaa
     7441 tatgttgccg ctgcgccatc agacggggaa ctcctcgccg atctttaact atcgttatga
     7501 ccgcagccgc gaagtgctgc acgatttaac ccgcatgggc gatgccgacg aatgggatgg
     7561 ctacaaaatg cgctacgtca acccggtcac cggcggctat ccgatgccgt cgatgggcgc
     7621 tttcctgcaa ctgctaccga aagggttctc ctcccgcgtg gcgcgcacca ctgacagcac
     7681 tatctaccat gtagtggaag gtagcggaca agtcaccatc ggcaatgaaa tcttcacctt
     7741 tagtgcaaaa gatatctttg tggtgccgac ctggcacggc gtgtcattcc agactacgca
     7801 agataccgtt ttattcagtt tttcggacag accggtacaa gaagccctgg ggcttttccg
     7861 cgaagcgcgt tattaatttg ggagagagaa aatgactcaa tatgtattcg aaccacaagc
     7921 gcccgttacc gtaccggttg ttggcagtga taaacaattc cccgtgcgtc gcgtttactg
     7981 cgtcgggcgt aactatgccg cccacgcccg cgaaatgggc tttgatccgg atcgtgaacc
     8041 acccttcttc ttctgcaaac cagcagatgc cgttattccg gtcgccgctg gcgaaacgct
     8101 ggcgctgcca tatccggcgc agaccgataa ctatcattac gaaattgaac tggtagtggc
     8161 gattggtaaa aaaggcagcg atatcccgct ggaacacgcc catgaatatg tctggggtta
     8221 tgccacgggt cttgatatga ctcggcgtga ccgtcaaatg gaaatgcgcc agatgggccg
     8281 cccgtgggaa atcggtaaag cgtttgatct ctcagcgccc atcgcgccgc tgcacaaagc
     8341 ccgcgatatc ggcggtattg ataatgcgcc aatctggtta caggttaacg gcgaagatca
     8401 tcagcgcagc gatattcgcc atctcatctg gtcggtgaat gaaaccatca gctatctgtc
     8461 aggcttcttt gagctgcaac cgggcgatct gatcttcacc ggtacgccgg aaggggttgg
     8521 cccggtagtg aaaggtgatg tgatcacagg caacgtcgag ggcttaacgc ctatcgcggt
     8581 gaagattgtc tgaggtgcat gatgaagctg tacagttttt ttaatagttc agcgtcgtac
     8641 cgggtacgca ttgcgctggc gctgaaaggc attaattacc agaccgaagg ggtgaatatc
     8701 cgtattggtc agcaaaatga actggcctat cggcggatga atccggtcgg gctggtgccg
     8761 acgctgctga ctgacgaggg cgaatcactc ggacaatcgc tggcgattat tgactggctg
     8821 gagaggcact acccgcaagt tccgctggtg ccgcaggaag aaccggcccg caacaaggtg
     8881 ctggaaattg tctatgccat cgcttgtgat attcatccat tgaataactt gcgcgtgctg
     8941 cgctacctga ccgaagaact gaacgtcagt gaagaagaaa aaaaacgttg gtatgcgcac
     9001 tggatccagc aagggttaag cgcggtggaa caactgttgc gccagaacca gtcggggcaa
     9061 ttttgtgtgg gcgagacgcc gacgctggca gattgctgcc ttgtcccgca gtgggcaaac
     9121 gcgctacgca tgaactgcga tttgagcggg tatccacgct gcaaagcggt gtacgacgcc
     9181 tgcacacagt tgccagcgtt cattgccgca gcgccagaaa atcaacaaga taaaatttca
     9241 gcctgaccag gagaactata atgacgaaag tgacacgcgc agtaattgtg ggaggcggga
     9301 tcggcggtgc ggcaactgcg ctgtcactag cccgcctggg gatcaaagtg gtgctgctgg
     9361 aaaaggcgca tgagatcggt gagattggcg cgggcatcca gttggggcca aatgcgtttt
     9421 cggcgctgga cagcctcggc gtcggcgaag ttgcccgcca gcgcgcggtg tttaccgatc
     9481 acattaccat gatggatgcg gtgaacgccg aagaagtggt gcgcattgaa accgggcagg
     9541 cctttcgtga ccatttcggc ggtccttacg cggtgatcca ccgggttgat attcacgcct
     9601 cggtgtggga agcggtactg acgcatccgg acgtggaata tcacacctcc accaacgtcg
     9661 tggatattcg cgagacggaa gacgatgtca ccgtgtttga cgatcgcggc aatagctgga
     9721 ccggcgatat tctgattggc tgtgacggcg tgaagtcagt ggtgcgccag agcttattgg
     9781 gcgatgcgcc gcgtgtcacc ggacatgttg tctaccgtgc ggttatcgat tgtgccgata
     9841 tgccggaaga tttgcgcatt aacgcaccgg tactgtgggc ggggccgcac tgtcacctgg
     9901 tgcattaccc tttgcgcggc ggcacgcagt ataacctggt ggtgacattc cacagccgcc
     9961 agcaggaaga gtggggcgtg aaggatggca gtaaagagga agtgctctct tactttgagg
    10021 ggattcaccc gcgtccgcgc cagatgctgg ataaaccgac ttcatggcga cgctggtcaa
    10081 ctgccgaccg tgaaccagtc gcgaaatggg gcactaaccg cattacgctg gtgggcgatg
    10141 cagcccatcc agtggcgcaa tatatggcgc aaggtgcctg tatggcgctg gaagacgcgg
    10201 taacgctggg taaggcgctg gcgcagtgtg agggtgatgc ggcgcaagcc tttgcgctgt
    10261 atgagtcggt acgtattccg cgcaccgcgc gtattgtctg gtcgacccgt gagatgggac
    10321 ggatttatca cgccgcggga gtagaacgcc aggttcgtaa cctgctgtgg aaagggaaat
    10381 cgcaggcaga gttttatcgc ggcatggagt ggctgtacgg ctggaaagaa gataactgtc
    10441 tgcaaccacg ctgagtactt cttcacctaa ccaggcgcac gggtgatctc cttgcccttc
    10501 ggagaggagc tattaagtcc tgttgatttc aacagggctc tctcctctcc ctttagcctc
    10561 ctttaaaata aggtatcatc agaaatcctt ctttcaacaa atagcacgcc acttcaagca
    10621 aatcgtctcc agagctattc agtgttacgt cctgtttttc cagtgtaaat catcaccaga
    10681 ttatgtctga aaagctcgtt acctacactg atcagtgtta gttgttttgg caaaaatatc
    10741 tcacaccggt tttcatcatc aacaagcact atacgcaacc aacaacacaa aaaggaacac
    10801 accatgcccc cacacaaaat ccgccaggag tggctggcgc tggacgagac cgcagaagtc
    10861 atcctacagc acagcatcac gttgcaatcc gccgacctta tccgccattc ttttatttgg
    10921 cgcaaccgca gtatctgtct gtgatgttgg tgttactgcc aagcgcttta tggttcggga
    10981 taacggcatg tatggtagca agtagcttta agcgatggtt ataagttgct caaaaaatcg
    11041 gtcctcacta caatacgaat tttatcaggt atggaattcg gattatgaat aaatctgtca
    11101 taaagctgtt gaataaaaaa
//
LOCUS       DAPHDW010000142        11063 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.144, whole genome shotgun sequence.
ACCESSION   DAPHDW010000142 DAPHDW010000000
VERSION     DAPHDW010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11063
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.144"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(45..1874)
                     /gene="glmS"
                     /locus_tag="RH057_003698"
     CDS             complement(45..1874)
                     /gene="glmS"
                     /locus_tag="RH057_003698"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HDV3130007.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEVNAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            complement(2036..3406)
                     /gene="glmU"
                     /locus_tag="RH057_003699"
     CDS             complement(2036..3406)
                     /gene="glmU"
                     /locus_tag="RH057_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HDV3130008.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDSTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGSKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            complement(3757..4176)
                     /gene="atpC"
                     /locus_tag="RH057_003700"
     CDS             complement(3757..4176)
                     /gene="atpC"
                     /locus_tag="RH057_003700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HDV3130009.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            complement(4197..5579)
                     /gene="atpD"
                     /locus_tag="RH057_003701"
     CDS             complement(4197..5579)
                     /gene="atpD"
                     /locus_tag="RH057_003701"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HDV3130010.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            complement(5606..6469)
                     /gene="atpG"
                     /locus_tag="RH057_003702"
     CDS             complement(5606..6469)
                     /gene="atpG"
                     /locus_tag="RH057_003702"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HDV3130011.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            complement(6520..8061)
                     /gene="atpA"
                     /locus_tag="RH057_003703"
     CDS             complement(6520..8061)
                     /gene="atpA"
                     /locus_tag="RH057_003703"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HDV3130012.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            complement(8074..8607)
                     /gene="atpH"
                     /locus_tag="RH057_003704"
     CDS             complement(8074..8607)
                     /gene="atpH"
                     /locus_tag="RH057_003704"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HDV3130013.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLAFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRAGDMVI
                     DGSVRGRLERLADVLQS"
     gene            complement(8622..9092)
                     /gene="atpF"
                     /locus_tag="RH057_003705"
     CDS             complement(8622..9092)
                     /gene="atpF"
                     /locus_tag="RH057_003705"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HDV3130014.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            complement(9154..9393)
                     /gene="atpE"
                     /locus_tag="RH057_003706"
     CDS             complement(9154..9393)
                     /gene="atpE"
                     /locus_tag="RH057_003706"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HDV3130015.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            complement(9440..10255)
                     /gene="atpB"
                     /locus_tag="RH057_003707"
     CDS             complement(9440..10255)
                     /gene="atpB"
                     /locus_tag="RH057_003707"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HDV3130016.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYSIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            complement(10264..10644)
                     /gene="atpI"
                     /locus_tag="RH057_003708"
     CDS             complement(10264..10644)
                     /gene="atpI"
                     /locus_tag="RH057_003708"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HDV3130017.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
BASE COUNT         2644 a         3021 c         2734 g         2664 t
ORIGIN      
        1 aaaagggcca ccggtcggca gccctttttt atctatgata gtgattactc aaccgtaacc
       61 gattttgcca ggttacgcgg ctggtcaacg tcggtgcctt tgatcagcgc gacatggtaa
      121 gccagcagct gcagcggaac ggtgtagaag atcggtgcaa tcacctcttc cacatgcggc
      181 atctcgatga tgtgcatgtt atcgctactt acaaaacccg catcctgatc ggcgaagaca
      241 tacaactgac cgccacgcgc gcgaacttct tcaatgttgg atttcagttt ttccaacagt
      301 tcgttgttcg gtgcaacgac gataaccggc atatccgcgt caatcagcgc cagcgggccg
      361 tgtttcagct cgcctgcagc gtaggcttca gcgtgaatgt aagagatctc tttcagtttc
      421 aatgcgcctt ccagcgcgat tgggtactga tcgccacggc ccaggaacag cgcgtgatgt
      481 ttgtcagaga aatcttctgc cagagcttca atgcgtttgt cctgagacag catctgctca
      541 atacggctcg gcagcgcctg cagaccatgc acgatgtcat gttcaatgga ggcatccaga
      601 cctttcaggc gagacagctt cgccaccagc atcaacagca cagttaactg agtggtgaat
      661 gctttagtgg atgccacgcc gatttccgta cccgcgttgg tcatcagcgc cagatcggat
      721 tcgcgcacca gagaagaacc cggaacgtta cagattgcca gcgaaccaag gtaacccagc
      781 tctttcgaca gacgcaggcc cgccagggta tccgcggttt cgccagactg tgacaaggtt
      841 atcatcaggc tgttacgacg cacggcagat ttgcgatagc ggaattcaga ggcgatttcg
      901 acgtcgcacg gaatacctgc cagcgattca aaccagtaac gggaaaccat accggagtta
      961 taagaagtac cacaggcgag gatctgaata tgctcaacct tcgacagcag ttcgtcggcg
     1021 ttcggtccca gctcgcttaa atcaacctga ccgtggctga tgcgcccggt aagggtgttt
     1081 tttatcgcgt tcggctgttc gtagatctct ttctgcatgt agtgacggta aataccttta
     1141 tcgcccgcgt catattgcag attggattcg atatcctgac gttttacttc cgcgccagtt
     1201 ttatcgaaga tgtttaccga acggcgagta atttccgcaa tatcgccctc ttcaaggaag
     1261 ataaagcgac gggtcaccgg caacagcgcc agctggtcag aagcgataaa gttttcgccc
     1321 atacccaggc caatcaccag cggactacca gaacgtgccg ccagcagggt atccgggtga
     1381 cgggagtcca tgatcactgt accgtacgca ccacgcagtt gcgggatagc acgcagaaca
     1441 gcctcacgca gagtaccgcc ctgtttcagc tcccagttca ccagatgggc aattacttcg
     1501 gtgtctgttt cagaaacgaa ggtatagcca cgcgctttta actcttcgcg cagcggttca
     1561 tggttttcga tgatgccgtt atgcaccacc acaatgtgtt cagaaacatg cggatgcgca
     1621 ttcacttctg aaggttcacc gtgggtcgcc cagcgagtat gagcaatacc cgtaccgcca
     1681 tgcagaggat gttcttccgc tgcctgagcc agcatctgga ctttaccgag gcgacgcagg
     1741 cgggtcatat gaccttccgc atcaacaacg gccagaccgg cagagtcata tccgcggtat
     1801 tccagacgac gtaaaccttc aagaaggatt tctgctacat cacgttgcgc gatcgcgcca
     1861 acaattccac acatagtttt tgattccgat ttatatcgtt gtcggtcaac ctgtatgccc
     1921 gttttttcgg gcgccccgag ccttgtagag agtggggtta tttttatagt tactgcttgt
     1981 gggagggaga ttatgttatc tcctcatccc atgtgaccgg attacccggc cagaatcact
     2041 ttttctttac cggacgacgc cagccttctt tctgagtctg cggcacacgg ctgatagcta
     2101 atgcattttc gccgacatta cgcgtcacag ttgtacccgc agcaatggtc gcgcctttgc
     2161 ctactgttac cggggccacc agctgagtgt cggaaccgac aaacacatcg tcgccgataa
     2221 tggtcttaaa tttattcgca ccatcgtagt tgcaggtaat ggttcccgcg ccgatgttaa
     2281 cgttatcgcc aatttccgca tcgcccaggt aagtcagatg accagctttc gagcctttac
     2341 ccagacgcgc ttttttcatc tcaacaaagt taccgacgtg tgcaccttcc agcaactcag
     2401 caccaggacg cagtcgggca aacgggccaa tagtacaggc cgctgccaga ttcgcgtctt
     2461 ccacaacggt atacggactg atttcgcaat catcgccaat cacgctgttt ttaatcacgc
     2521 aaccggtgcc gattttcacg cgatgaccga gagtcacgtt gccctcgatg ataacgttag
     2581 tatcaatttc aacatcgcgc ccgtgagtaa gcgtaccgcg caaatcaaaa cgcgccggat
     2641 cgcgcagcat aacgcctgct aacagcagtt tttcagcctg ttcggactgg taaacgcgct
     2701 ccagacggga gagttgcagg cggttattca caccttctac ttcgcttaaa cgttgcggat
     2761 gaacggcgac gatttcacgc ccttcctgat acgccagcgc aataatgtcg gtgatgtagt
     2821 attcgccctg agcattattg ttggtcagct tcgccagcca gcgtttcata tctgcgccgt
     2881 tagcgatcag aatgccggtg ttgatctcct gaatctgacg ctgctcgtcg gtggcatctt
     2941 tgtgctcaac aatgccggta actttgccat tttcacgggt gatacgtcca taaccggtcg
     3001 aatcatccag tttcaccgtc agcagaccaa tgccaccctg cggtttagca tcacgcaggc
     3061 gctggagtgt ttcgacagag atcagcggca cgtcgccgta gagcattaaa atgtcttcat
     3121 catcggcaaa gaaaggtgcg gcctgctgca ttgcatgacc cgtacccagc tgctctgcct
     3181 gaagcaccca gttaaggttg tcgtctttca gcgcctgttt aagcagatcg ccgccgtgac
     3241 cgtacaccag gtgaacgtgc gctgcgccta attcattcgc agcatcaatg acatgctgaa
     3301 ccatcgcttt cccggcaagg gtatgcagca ctttcggaag atcggaatac atgcgcgtgc
     3361 ctttgcctgc ggcaaggatc actacgctca tagcattatt caacatacgc gtcctgactg
     3421 taatttgaga acgaatttaa accgcttcac cttgaaaaaa ctacattttt ttcatcgtga
     3481 aatggacaga ggataaattg ttcaataacg gattatcccc gctgacgaaa gtgccatttt
     3541 cgaccatcgt cacgcctttt gtctgcagaa aattaaggca gtaccttcat ttaagcgtca
     3601 ggtggatgtt tttgctctta tttcgatcaa tgaataaacg ggaaaacagg ggtgttttat
     3661 atcagcagga tctatgtgaa cgctcttcag gacggatcac gcgtaaaaaa aagccagcct
     3721 gtttccagac tggcttttgt gcttttcaag ccggtgttac atcgcttttt tggtcaactc
     3781 gataacgcgc agctgcgcga tcgctttggc cagttccgca gacgcctgag cgtaatctac
     3841 gtcgccgtga gagctgctaa tgtgctcttc agccttacgt ttcgcttcca tggctcgcgc
     3901 ttcgtcgaga tcctggccgc gaattgcggt gtcggccaga acggtcacgt tgccaggctg
     3961 cacttcaaga atgccgccag acagatagat aaactcttcg tgaccgtgct gtttcacgat
     4021 gcgaatcata ccaggcttaa tggcggtgag cagcggtgcg tggccagggt agatccccag
     4081 ttcaccttcg ctacccgtta cctggatttt ctcgaccaga ccagagaaca tttgttgctc
     4141 tgcgctgacg acgtccaggt ggtaagtcat tgccatatca ccctccgatt aaggcgttaa
     4201 agttttttgg ctttttccac agcttcttcg atggaaccga ccatgtagaa cgcctgctcc
     4261 ggcaggtgat cgtattcgcc ttccatgatg cctttaaagc cacggatggt gtctttcagg
     4321 gagacgtatt tacccggaga accggtgaat acttctgcca cgaagaacgg ctgggacagg
     4381 aagcgctgga tcttacgagc acgcgctacc accagtttgt cttcttcaga cagttcatcc
     4441 atacccagga tggcgatgat gtctttcagt tcctgataac gttgcaggat ggactgaacg
     4501 ccacgcgcgg tgtcgtagtg ttcctgacca accaccagcg ggtccagctg acggctggtg
     4561 gagtccagcg ggtcaacggc cgggtagata cccagagacg cgatctgacg gctcagtacc
     4621 acggttgcgt caaggtgcgc aaaggtggtt gccggagacg ggtcagtcaa gtcatccgca
     4681 ggtacgtata ccgcctgtac ggaggtgata gaaccagttt tggtggaggt gatacgttcc
     4741 tgcagaacgc ccatctcttc cgccagggtc ggctgataac ctaccgctga aggcatacgg
     4801 cccagcagtg cggatacttc cgtaccggcc agggtgtaac gatagatgtt gtcaacgaac
     4861 agcagaacgt cacgaccttc gtcacggaat ttctcagcca tggtcagacc ggtcagagca
     4921 acgcgcagac ggtttcccgg cggctcgttc atctggccat acaccaggga tactttgtcg
     4981 ataacgttgg agtcggtcat ttcgtggtag aagtcgttac cctcacgagt acgttcacct
     5041 acgcccgcaa acacagagta accggagtgc tcgatcgcga tgttacgaat gagctccatc
     5101 atgtttacgg ttttacctac acccgcacca ccgaacagac caactttacc gcccttagcg
     5161 aacggacaca tcaggtcgat aactttgata ccggtttcca gcagttcctg agagtttgac
     5221 agctcttcgt aggaaggtgc tgcgcggtga atcgcccaac gctcttcttc accgatctcg
     5281 cctttcatgt cgaccggttc acccagtacg ttcatgatac ggcccagagt cgctttacct
     5341 accgggactt cgatcgggtg ttcgaggtct tttacatcca gaccgcgacg cagaccgtcg
     5401 gaggaaccca ttgcgatggt acgcacgata ccgccgccga gctgctgctg aacttccagc
     5461 accagacgct cattaccatt ttgcacctca agagcatcgt acacgcgcgg tacggcatcc
     5521 tgagggaatt cgacgtcaac tacggcgccg attacctgga caatctttcc agtagccatc
     5581 ttaaatcctc tacgaaataa cctgtttaaa ccgcggcggc ccccgagacg atctcggtga
     5641 gttcctgagt aatgctggcc tgacgagctt tgttgtatac caactgcagc tctttaatca
     5701 ggctgccgcc attgtcggtc gcggctttca tcgccaccat acgggcggcc tgctcgctgg
     5761 ccaggttttc aaccacgccc tgataaacct gagattcgac ataacgacgc agcagggtat
     5821 ccagcaacgc cttcggatcg ggttcgtaca ggtaatccca ggatttatgt ttcagatcat
     5881 catcatctga tgccggtaac ggcagcagct ggctgatggt cggaacctga gacatggtgt
     5941 taataaattt gttgctgaca atgtaaagct tgtccagacg gccttcgtcg taggcctgca
     6001 acatcacttt taccggaccg atcagttcgg acagggaagg gttatccccc atgccggtga
     6061 cctgggcaac aacattgccg cccacggagt tgaagaacga cacgcctttc gagccgatca
     6121 ttgcgaggtc gcattgaacg cctttgtcgg tccaggtctt catttccgcc agcagttttt
     6181 tgaacaggtt aatgttcaaa ccaccgcaca aaccacggtc ggtcgacacc accaggtagc
     6241 ccacgcgttt aacgtcgcgg tcttccaggt aagggtgctt atattccaga ttaccgtgtg
     6301 caaggtgacc aatcactttg cgcatggttt ctgcataagg acggctggcc gccatgcgat
     6361 cctgcgattt acgcattttg gaagcggcga ccatctccat cgctttagtg atcttttgcg
     6421 tgttctggac gcttgcgatc ttactacgta tctcttttgc gccggccatg agcttctcct
     6481 caatgccttg cggcctgccc taaggcaagc cgccagacgt taccaggatt gggttgcttt
     6541 gaaggaatcg aggatgcctt tcagcttgcc ttcgatttcg tcgttgtagc caccggtctg
     6601 gttgatctct tgcatcaacg gagcgtgatc acggtcgacg taagccagca gagcggcttc
     6661 gaagctgcca attttcgaca gttcaacatc cgccaggtaa ccacgttctg ctgcgaacag
     6721 aaccagagac tgctgcgcaa cggacatcgg cgcatactgt ttctgtttca gcagttcggt
     6781 cactttctga ccgtggtcaa gctgcttacg tgttgcatcg tcaaggtcgg atgcaaactg
     6841 agagaacgct gccagttcac gatactgtgc cagagcggta cggataccac cggacagttt
     6901 tttcatgatc ttggtctgtg ctgcaccacc aacacgggat acggaaatac ccgggttaac
     6961 cgcaggacga ataccggcgt tgaacaggtt ggtttccagg aagatctgac catcggtaat
     7021 ggagattacg ttggtcggaa cgaacgcaga aacgtcaccc gcctgagttt cgataatcgg
     7081 cagtgcggtc agagaaccgg ttttcccttt cacttcacct ttggtgaagg cttcaacgta
     7141 ttcggcgtta acacgtgcag cacgctccag cagacgagag tggaggtaga aaacgtcgcc
     7201 cgggaatgct tcacgtcctg gcggacgacg gagcagcagg gagatctgac ggtaagcaac
     7261 agcctgttta gacaggtcat cgtaaatgat cagcgcatct tcaccgcggt cacggaagta
     7321 ttcgcccatt gcgcaaccgg catacggtgc caggtattgc agtgcagcgg attcagacgc
     7381 ggttgctacc acaacgatgg tgttagccag tgcgccgtgc tcttccagtt tacgtaccac
     7441 gttagaaatg gtggacgctt tctggccgat agcgacatag atacatttga taccggaatc
     7501 gcgctggttg atgatggcat cgatagccag tgcggtttta cctgtctgac ggtcaccgat
     7561 gatcaattca cgctgaccac gaccgattgg gatcatggag tcaacggctt tataaccggt
     7621 ctgtaccggc tgatctacgg actgacgttc gataacgccc ggagcgattg cttctacagc
     7681 agagaagccg tcgtgatcca gcggaccttt accgtcgatt ggtgcaccca gagtgttaac
     7741 cacacggccc agcaggccac ggccaaccgg aacttccagg atacggccag tacacttaac
     7801 tttcatgcct tcggcaaggt cagcgtacgg acccataaca accgcaccta cagagtcgcg
     7861 ctcgaggttc agtgcgatag cgtaacggtt acccggcagg gagatcattt caccctgcat
     7921 acaatcggcc aggccgtgaa tgcggataac accgtcactt acagaaacaa tagtaccttc
     7981 gttgtgagct tcactcacaa cattgaactg agcaatgcgc tgcttgatca gttcgctgat
     8041 ttcggtggaa ttcagttgca tgctccagtc cccttaagac tgcaagacgt ctgcaaggcg
     8101 ctcaagacga ccgcgtacgc tgccatcaat gaccatatca cccgctcgga tgataacgcc
     8161 tgccattaca gacttatcga ttttgcaatt cagcttaact ttgcgtgaca gacgtttttc
     8221 catcgcagca gaaattttcg cgagctgttg ttcactcagt gcggcagcgg aaatgacgtc
     8281 tacctcagcg gtagcctcac tcacggcacg aaggtgaata aactgctcca gaacatccgg
     8341 gagcgcgtta agacgaccat tttcagccat tacccgaatc aggttctgac cgttttcgtc
     8401 cagttgctca ccacaaactg cgataaacga ctcggcgagc gtttctggcg caagcgcgcc
     8461 agagagaagc tctgccattt gttcgttttt ggttacctcg gcggcaaacg ccagcatgtc
     8521 ctgccagcgt tctacacttt ggtgttcgac ggcaaagtca aaagctgctt tggcgtaggg
     8581 gcgagctacc gtaataaatt cagacatcag cccctccctc cttacagttc agcgacaagt
     8641 ttatccacga tgtcgctgtt agcagcttca tccacggaac gttcgatgat cttctcggcg
     8701 ccagcaacag ccaggatagc aacttgctta cgcagctctt cacgggcacg tttacgctcg
     8761 gcttcaattt ctgcctgcgc ctgggccacg attttagtac gttcctgttc tgcctcagct
     8821 ttcgcttcgt ccagaatctg cgagcggcgt ttgttcgcct gctcgatgat tacctgggct
     8881 tccgctttcg cttttttcag ctggtcggtc gcgctggcct ttgcaaggtc aaggtcctta
     8941 tgtgctcgtt ctgcggaagc aaggccgtca gcaatttctt tttgacgttt ttcgatggct
     9001 gccattaatg gcggccatac gtacttcatg cagaacagaa cgaacaggac aaacgcgatg
     9061 gcctggccga ggattgttgc gttaagattc acagcacaat gcctctattt agttaacgtt
     9121 ctgatattgc tctttaaata aaagcaacgc ttactacgcg acagcgaaca tcacgtacag
     9181 acccagacct acagcgatca tcgggatagc atccaccaga cccataacga taaagaactg
     9241 agtacgcagc agaggaatca gatcaggttg acgcgctgcg ccttccagga atttaccccc
     9301 gaggatgccg ataccgatcg cagcaccgat tgccgccaga cccatcatca cagcggcagc
     9361 catgtacagc agatccatat tcaggttttc catgacagtc tccagtttgt ttcagttaaa
     9421 acgtagtagt gttggtaaat taatgttctt cagacgccat cgacagatag acgatcgtca
     9481 gaaccatgaa gatgaaggct tgcagcgtaa tgatcaggat gtggaaaatg gcccacggca
     9541 cattcaggat ccactgtgac caccacggca acagaccagc aatcagaatg aaaatcagct
     9601 caccggcata catgttaccg aacagtcgca aaccaagtga aactggtttg gacagcaggc
     9661 ttaccccttc aaggattaag ttgacaggaa tgaacgccca gtgattgaac ggctgcagcg
     9721 tcaactcttt cgtgaagccg ccgatgcctt tcattttgat gctgtagaac agaatcagga
     9781 taaatacgcc caatgccata gacagcgtta cgttcacgtc cgcagacgga accacacgca
     9841 gtgcaggcag acccagtaca tgttcagcaa tgtacggcag caggtcgata ggcagtaaat
     9901 ccatcaggtt catcaggaat acccagacga agatcgtcag ggccagcgga gcaatcagct
     9961 tgcttttgcc atggtacatg tctttcacgc taccattaac aaagccgatc accagctcaa
    10021 tcgcggtctg aaacttacct ggcacaccgc tggtcgcctt tttggctacg ctacggaata
    10081 aaaccaggaa caacagaccc agcaccaccg agaagaacat ggagtcaata ttgattgtcc
    10141 agaaggtggc tggggggttt tgtggatcca ccagcgagaa tgtacgcagg tccagctgaa
    10201 ggttattcag gtggtgtcct atgtaatcct gcggcgtcat attttctgaa gccatgatgc
    10261 cttttaccct ttgttgttaa ttacagccgg tgccagtatc tgaaccacca gcaccaaaac
    10321 ccacgtaacg atcagcggca agaataccgc ctttaaaacc gccaacgcca ccaccagtaa
    10381 caccaacatc gccagaactt tgaaagcttc gccaaatgcg aatgtccagg ccacccggcc
    10441 tttcgctggt gtatgcgcct ggtgacgcca ggcaaatatc ataaacaaaa cgttaggcag
    10501 aaagactgcc aggcccccgc ttattgcaga gacgccccag aaggggtctt tgaggctgaa
    10561 cagcaatcca cttgctatca ccaccagtaa ctgaacgagc agaagcttcc gagcaacgtt
    10621 tcgactcacg agcgacacag acatcacgtt tttcactcct gctcccttcg aggtatgccg
    10681 cgtgtcgtat aaaactttct ttaaggctta gagtcaagca tcaaaaagcg gtcaaattat
    10741 acggtgcgcc cccgtgattt caaacaataa gtagccaaaa ggtgaataaa tgtttaaata
    10801 tttttccagt gcatacaatt gcgacttttc tgctaaccct gttcgatcat gaaaaactgt
    10861 aaataacgcg taaacactgg tgataaagcg tgcttcagat cacatattgc gcatgttcgc
    10921 gcacagcata tttatttact tggcaaatga tgcctttgca agtttatgat atttcagtct
    10981 aaaaacagat actgttttaa taaatgacat ttacacaaca aaaaccaccc attgacattt
    11041 ttaataatgt tttaacagcc aat
//
LOCUS       DAPHDW010000143        11055 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.145, whole genome shotgun sequence.
ACCESSION   DAPHDW010000143 DAPHDW010000000
VERSION     DAPHDW010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11055
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.145"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            53..847
                     /gene="hyxR"
                     /locus_tag="RH057_003709"
     CDS             53..847
                     /gene="hyxR"
                     /locus_tag="RH057_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001304859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR family transcriptional regulator HyxR"
                     /protein_id="HDV3130018.1"
                     /translation="MKKPLVIISACQFTRLALESLIPADRYIVRVYSNVTTEVEFVLK
                     TSCGYLLADIPSLPSGELVLLACLVRLRSDAGQWQVCLTGDSDWFDNAPASSLYNGFP
                     HIGTLLKASRYQNQIIRWLLRPLPGDMSDFWLLTARELSVLKILMQGMSFSEIARYEQ
                     RSSKTLHAIATQALRKLGLGTLSDFRLLYTGCGDSRVKRNIRLHARYVGQHSAHRLTQ
                     YVQNMVSGILYPEKVIVGKSINSDAISGKVLSGQRKHMSKKRPGVL"
     gene            1001..1762
                     /gene="hyxA"
                     /locus_tag="RH057_003710"
     CDS             1001..1762
                     /gene="hyxA"
                     /locus_tag="RH057_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein HyxA"
                     /protein_id="HDV3130019.1"
                     /translation="MRFVLFCPSGIVPAQFEALSTGSVCNITCIRTEEELRNKLRHRP
                     QSVVISAGRPAECAEMWFRFYRDHSFVVVLCVAPFFLPPDVSISGVLKNLRLLKTGMS
                     VERAISVANASGGFSGLKHAENLPVMDSYSVFMNEVNNRTKTIVMSERFPEKQKKVLS
                     LLLAGHSWEYSAQFLKTGIRQIWLAEQSLKKRWGIPDSMSLREALLLSSNKFHGDGNA
                     LEKTNAMTLRENGNTNRYSVVVNAGTQALSLHKYK"
     gene            1804..2886
                     /locus_tag="RH057_003711"
                     /pseudo
     CDS             1804..2886
                     /locus_tag="RH057_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308377.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
     gene            2908..4101
                     /locus_tag="RH057_003712"
     CDS             2908..4101
                     /locus_tag="RH057_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3130020.1"
                     /translation="MTKATVNTGTFASQNGTLIVDASSENTLDISGKASGDLRVYSAG
                     SLDLINEQTAFISTGKDSTLKATGTTEGGLYQYDLTQGADGNFYFVKNTHKASNASSL
                     IQAMAAAPANVANLQADTLSARQDAVRLSENDKGGVWIQYFGGKQKHTTAGNASYDLD
                     VNGVMLGGDTRFMTEDGSWLAGVAMSSAKGDMTTMQSKGDTEGYSFHAYLSRQYNNGI
                     FIDTAAQFGHYSNTADVRLMNGGGTIKADFNTNGFGAMVKGGYTWKDGNGLFIQPYAK
                     LSALTLEGVDYQLNGVDVHSDSYNSVLGEAGTRVGYDFAVGNSTVKPYLNLAALNEFS
                     DGNKVRLGDESVNASIDGAAFRVGAGVQADITKNMGAYASLDYTKGDDIENPLQGVVG
                     INVTW"
     gene            4285..4950
                     /locus_tag="RH057_003713"
     CDS             4285..4950
                     /locus_tag="RH057_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130021.1"
                     /translation="MREQIKQDIDLIEILFYLKKKIRVILFIMAICMAMVLLFLYINK
                     DNIKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFHTSPDVKNREIK
                     LEWSGDKRDLPTAEAEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSG"
     gene            complement(5196..5891)
                     /locus_tag="RH057_003714"
     CDS             complement(5196..5891)
                     /locus_tag="RH057_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HDV3130022.1"
                     /translation="MDNRGEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAGRGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(5884..7311)
                     /locus_tag="RH057_003715"
     CDS             complement(5884..7311)
                     /locus_tag="RH057_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HDV3130023.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVANAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVWFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILSHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(7322..8041)
                     /locus_tag="RH057_003716"
     CDS             complement(7322..8041)
                     /locus_tag="RH057_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HDV3130024.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAVKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVAHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
     gene            complement(8571..9425)
                     /gene="rclR"
                     /locus_tag="RH057_003717"
     CDS             complement(8571..9425)
                     /gene="rclR"
                     /locus_tag="RH057_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RclR"
                     /protein_id="HDV3130025.1"
                     /translation="MDALSRLLMLNAPQGTIDKNCVLGSDWQLPHGAGELSVIRWHAL
                     TQGAAKLEMPTGEIFTLRPGNVVLLPQNSAHRLSHVDNESTCIVCGTLRLQHSARYFL
                     TSLPETLFLAPVNHSVEYNWLREAIPFLQQESRLAMPGVDALCSQICATFFTLAVREW
                     IAQVNTEKNILSLLLHPRLGAVIQQMLEMPGHAWTVESLASIAHMSRASFAQLFRDVS
                     GTTPLAVLTKLRLQIAAQMFSRETLPVVVIAESVGYASESSFHKAFVREFGCTPGEYR
                     ERVRQLAP"
     gene            9651..10976
                     /locus_tag="RH057_003718"
     CDS             9651..10976
                     /locus_tag="RH057_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HDV3130026.1"
                     /translation="MNKYQAVIIGFGKAGKTLAVTLAKAGWRVALIEQSNAMYGGTCI
                     NIGCIPTKTLVHDAQQHTDFVRAIQRKNEVVNFLRNKNFHNLADMPNIDVIDGQAEFI
                     NNHSLRVHRPEGNLEIHGEKIFINTGAQTVVPPIPGITTTPGVYDSTGLLNLKELPGH
                     LGILGGGYIGVEFASMFANFGSKVTILEAASLFLPREDRDIADNIATILRDQGVDIIL
                     NAHVERISHHENQVQVHSEHAQLAVDALLIASGRQPATASLHPENAGIAVNERGAIVV
                     DKQLHTTADNIWAIGDVTGGLQFTYISLDDYRIVRDELLGEGRRSTDDRKNVPYSVFM
                     TPPLSRVGMTEEQARESGADIQVVTLPVAAIPRARVMNDTRGVLKAIVDNKTQRILGA
                     SLLCVDSHEMINIVKMVMDAGLPYSILRDQIFTHPSMSESLNDLFSLVK"
BASE COUNT         3030 a         2584 c         2545 g         2896 t
ORIGIN      
        1 acattttgaa tcaaaatgtc aggttttgta gcaatggcgt ggacgagtct gaatgaagaa
       61 gcctctggtt attatcagtg cctgtcagtt caccaggctg gcactggagt ctctcatccc
      121 agcggacagg tatatcgtgc gggtgtacag caacgtcact acagaggttg aattcgtttt
      181 aaagacctcc tgtggctacc tgttggctga tatcccttcg cttccttcgg gggagttagt
      241 tcttctggct tgtcttgtcc gcttgcggag tgatgccggg caatggcaag tctgcctgac
      301 cggtgactcc gactggttcg ataatgctcc tgcttcaagt ctgtataacg gcttcccaca
      361 tatcggcacc ttgctgaaag cgagtcgtta tcagaatcaa attatccgct ggttactacg
      421 tcctttaccc ggcgatatga gtgatttctg gctcctgact gcacgggagt taagcgtgct
      481 taagattctg atgcagggca tgagtttcag tgagattgcc cgttatgaac aacgcagttc
      541 aaagactctg catgcgattg ccacacaggc attaaggaaa cttggactgg gaacactaag
      601 cgatttcagg ctgctctata caggatgcgg tgacagcaga gtcaaacgga atatacggct
      661 acatgcaaga tatgtcggac aacattccgc tcatcgactc actcagtatg tgcagaacat
      721 ggtgtccgga attctttatc cagagaaagt tatcgttggt aaatcaataa attctgacgc
      781 catatccgga aaagtgcttt ccgggcaaag aaaacatatg tctaaaaaaa gaccaggagt
      841 gttatgacac agcagcgttg ttactaccac ccactgcggc gcgtacggac tcgctacagt
      901 gtaggggcca aaatatttct actgctctgg gctgcaggag tgggaggcac gctgtacagc
      961 ctgtacagcg tgctggcgta ctgacatctg tgagggaagg atgcgtttcg ttcttttctg
     1021 tccctcagga attgtacctg cacagtttga agctctatca accggttctg tctgcaatat
     1081 tacctgcatc agaacagaag aagagttacg caataaattg cggcacaggc cgcagagtgt
     1141 cgtgatatcg gcagggcgac ctgccgaatg cgctgaaatg tggttccgat tttatcggga
     1201 tcactcattc gtggtggtgt tgtgcgtcgc gccctttttt ctgccaccgg atgtgagtat
     1261 ttctggcgtt ctcaaaaacc tgaggttact gaaaacagga atgtcagtag agcgtgctat
     1321 cagtgttgca aatgcctctg gtgggttttc aggactgaaa catgcagaaa atttgccagt
     1381 tatggacagt tactctgtct ttatgaatga ggtgaataac cgcacaaaga caatcgtcat
     1441 gtcagaacgc ttcccggaaa agcaaaagaa ggtgttgtcg ttgcttcttg ctggacacag
     1501 ctgggaatac agtgcgcagt ttctcaaaac ggggataaga cagatatggc ttgctgagca
     1561 gtcgctaaaa aagcgttggg gaataccgga cagcatgtca ttgcgggaag ccctgctgct
     1621 gagcagcaat aaatttcacg gtgatggaaa tgcattggaa aaaacgaatg ccatgacatt
     1681 gcgtgaaaac ggaaatacca acaggtattc cgtggtggta aatgcaggga cgcaggcttt
     1741 atctctgcat aaatataaat aagtgggaaa ttgaaattag ttaccagaat ggagaatttc
     1801 ttcatgaaaa acagtaaggc attttaccgc agcgcattag cgacagctat tgttatggct
     1861 ctttctgcac cagcattcgc tgctgataac gcggtatcaa ctacaccggt tacactggat
     1921 aaagataaga caactctgga tcaagatgtt gttattagca atacagcaga ccaacagatt
     1981 acagccgtaa caattaatgc ggcagataaa gatcttaatg ttacttttgg cggtcatgat
     2041 attactgctg aatcaacggc agacaaaaaa ttccttgaag gtgtaaaagt tagcggtgac
     2101 aaaaatattg tgattaatgc tacaggctcc accatcacag ctcaaggtga aggcacctat
     2161 gtccggactg caatggtcat ttcttcaact ggcgatgttg ttgttaatgg cggtaatttc
     2221 gttgcaaaaa atgaaaaaag tagtgcgaca ggaatatctc tggaaggggc cacgggaaat
     2281 aatgtaacgc taaatggtac aaccataaat gctcaaggta ataagagttc cagcaacggc
     2341 tctacggcaa tttttgctca aaagggtagc gtattgaatg gttttaaagg tgatgcaacc
     2401 gacaacatta cccttgctgg ctcaaatatt attaatggcc ggattgaaac aatagttatt
     2461 gccaaggaga atacgggaac tcatacagtc aatctgaata ttaaggatgg ctcagtaatt
     2521 ggggcggcta ataataaaca aacaatttat gcttctgctt cggcacaagg agcaggttca
     2581 gcaacgcaaa atttaaattt atctgtcgcc gattcaacca tctactctga tatccatgcc
     2641 ctttctgcaa gcgagaattc agccggtacc acaacaaatg taaacatgaa cgttgcccgc
     2701 tcttactggg aaggtaatgc ttataccttc aatagcggcg ataaagcggg tagtaatctg
     2761 gatataaatc tttccgatag ctcagtctgg aaaggcaaag tttcaggggc aggagatgcc
     2821 agtgtatctc tgcaaaacgg gtctgtctgg aatgttacgg gatcctcaac tgttgatgct
     2881 ctggcagtaa aagacagtac ggttaatatc acgaaggcta cagtcaatac tggcacgttt
     2941 gcttctcaga acggcactct gattgttgat gcctcttctg aaaacactct ggatatcagc
     3001 ggaaaagcga gcggtgactt gcgtgtttac agtgcgggtt cattggatct tatcaatgaa
     3061 caaacggcat ttatttctac cggcaaagac agcactctaa aagccacagg cacaacggaa
     3121 ggtggtctgt atcaatatga cctgacacag ggagctgatg gtaactttta tttcgtaaaa
     3181 aacacgcata aagcatccaa cgccagctcc ctgattcagg caatggcagc agctccggct
     3241 aacgttgcta atctgcaggc tgacacgctc tcagcccgtc aggatgctgt ccgtctgagc
     3301 gaaaatgaca agggcggcgt atggattcag tactttggcg gtaaacagaa acataccacc
     3361 gcgggaaatg catcctatga cctggatgta aacggtgtaa tgctgggtgg tgatacccgc
     3421 ttcatgactg aagatggtag ctggctggca ggtgtagcga tgtcttctgc gaaaggtgac
     3481 atgaccacca tgcagagcaa aggcgacact gaaggttaca gcttccacgc ttacctgagc
     3541 cgccagtata acaacggtat cttcattgat actgctgcac agtttggtca ctacagcaac
     3601 acggcagatg ttcgcctgat gaatggtggc ggtaccatca aagctgactt taacaccaat
     3661 ggtttcggtg cgatggttaa aggcggttac acatggaaag acggtaatgg cctgtttatt
     3721 cagccatatg ccaaactgtc tgcgctgacg ctggaaggtg tggattatca gctcaacggc
     3781 gtggacgttc attctgacag ctataactct gtgctgggtg aggccggtac gcgcgtgggt
     3841 tatgacttcg ctgtgggcaa ctcgaccgtt aaaccttatc tgaatctggc cgcactgaac
     3901 gaattctctg atggcaacaa agtccgtctg ggtgatgagt ctgtcaatgc cagcattgac
     3961 ggtgcagcat tccgcgtggg tgcaggtgta caggctgata tcaccaaaaa catgggagca
     4021 tatgcaagcc ttgactacac caaaggtgac gacattgaga acccgctaca gggtgtagtt
     4081 ggtatcaatg tgacctggta agaataaagc agtcggttcc cctgtcgact gttatacccc
     4141 ctgcaggcag catactgctg cctgcaggct ataaacatcc agtattcatg atgctggttt
     4201 tttgctgata tttaccccga acattcctga tacaggctat taatacaacc ttatctgcgg
     4261 cagattttat tttctggata tattatgcgt gaacaaatca aacaggatat cgatctgatt
     4321 gagattttat tttatctgaa gaaaaagatt cgtgttatcc tttttattat ggctatatgt
     4381 atggctatgg tgctgttgtt tctgtatatc aataaagaca atataaaagt gatttacagc
     4441 ctaaaaataa accagacaac gccaggtata cttgttagct gtgatagcaa taataatttt
     4501 gcctgtcaga ccacaatgac tgaagatgtt attcagcgaa ttactacatt ttttcacacc
     4561 agcccagatg tcaagaacag agaaataaag ctggaatggt caggagataa gagagattta
     4621 ccaactgctg aagcggaaat atctcgcgtg caggcgtcta ttatcaaatg gtatgcgtca
     4681 gaataccata atggcaggca agttctcgat gagatacaaa caccttcagc aattaacagt
     4741 gagctttata caaaaatgat atacctgacc aggaactggt cattgtatcc gaacggtgat
     4801 ggctgtgtaa ctataagctc accagaaata aaaaataaat accctgctgc catttgcctg
     4861 gctctgggat tttttctaag cattgtaatt tctgtaatgt tttgccttgt caaaaaaatg
     4921 gtagatgaat accaacaaaa ctctgggtag taataaacag cattctgata gagagtgcct
     4981 gtataaagct gaagtgacaa tgaccgggaa gggaacccgt tttcaggaat ggatgcctgt
     5041 ctttttcact gtaaatatcc tggcattact aatgccagga tatttacaga agagcagcat
     5101 aaccatgacg gaacggggtt aagtcaggtt gtccttaacg ctattttcaa cctcgtatgc
     5161 cttcttcagg tttatgttca gatctcatat ctctctcagc aatcctcaat aatcagatac
     5221 accgctttca ccggaccatg aaccccgacg actttgataa gctcaatatc cgccgttgaa
     5281 ctagggccgc taatgatgtt aatgcaggac ggcattcgtt caccggcctg cgctttctga
     5341 tgcaattttt ctgcgagttg cgctacacgc ggcaggattg tgcttttacg caggataaaa
     5401 agagaagatt ccgggagcag gctcaatgaa cgcccgcgcc cggcggcgga aaaaagaacc
     5461 acgcctcccg attcggttaa accatattca gcatacacga cacccacttt agcctgctcg
     5521 gcctgcgaga tattctcggc acctttcgcc ggatcccaaa caacggcatt gcattcctgc
     5581 tgcaaacgtt cactaatccc caattcctcg agcctcgtgt caccgctaat cacgaccgac
     5641 tgatctccca gctctttaca cagacgtatt gcagcttctg ccgccttcgc ctcgctggtc
     5701 agctcacagc gcgtcaacat aacatcgctg gcaaactgaa taaacgcgtc acagcgctgc
     5761 tgttggttaa gttgggtaag ccgctcgtta gcatagttgt taagcggcgc atcttctgct
     5821 tgcggttcaa gtcgcagcgg gcgacccagt gcctgagcaa cgttattcaa aaattcgcct
     5881 cgattatcca ttctttttct cctgcgcctg atgtttctta aaccaactac ggaaactctc
     5941 tccgtcagct tcaggaagat cgcgtgcttc catccagtcg ctaatcgcgc caaatttgag
     6001 tggtgttttg ccgccattga taaaccagct tgccgcatga gcgccggcca tcatcccgac
     6061 tttccacaat cctggatgac tattggcata agcgaacatt tttatcgccc gttgctctgc
     6121 ttttgcggtg atcccttttt cagccatcac ccgacgatga ctcaaaatca gttttgacag
     6181 cggaatacgc accggacaca cgctgtcaca agcagtgcat aaagagcagg cgtagggtaa
     6241 atctttaaaa tctttatagc cgccaagtag cggagaaatc accgcaccaa ttggccctgg
     6301 ataaatagag ccatatccat gaccgccaat atggcgatat gccggacaag tattcataca
     6361 agccccgcag cgaatacagc gcagcacatc ccgaaattca gaggccagca cctcagaacg
     6421 cccgttatcg acaataacca gatgaaactc ttcaggacca tcaacgtgcc ccgcttcgcg
     6481 cggtcctgtc agccaggtgt tgtatcccgt caaacgtgca ccaacggcac tgcgcgccag
     6541 catggtgatc aatacatcta cctcggcaaa cgtgggggca atacgctcca ttcccatcac
     6601 tgcaatatgc gttttaggca gcgtggtaca cattcgcgca ttaccttcat tggtcaccag
     6661 gcataccgaa cctgtctctg ccaccgcgaa attacagccg gtaataccta tttcagcact
     6721 gaggaaatct tcgcggattt tttgccggat gaataaggtc attgcttcag gcgtttccgg
     6781 cccctcatag cccagacgtt cgtgtagcac tcgacggatc tgatggcgat ctttatgaat
     6841 tgccgggacc acaacatgag atggcggatc ttgatccagc tggagaatat attcacccag
     6901 atcggtttca atcacctgaa tgccagcatc ctgcaataca tgattgacac caatctcttc
     6961 ggtcaccatc gatttagatt tcaccacctt ccgggcattt ttgcgttggg caacctgtaa
     7021 aatgtagcgg gtagcgtctt ctttggtttt tgcaaaccag acgtgaccgc cgttttgcgt
     7081 cactttttct gagagctggt acagataagc gtcgagatta ctcagaacat gatcacgtat
     7141 ctgggcagcc cgatcgcgcc actcctccca gtgccccaat tcatcgacca ttttttgccg
     7201 atttgcccca atacgctgct gcgcgtttgc caccgctttg cgcatgatcg gatcttcaat
     7261 ttgctgacgg atgcgtgtct taaaatctgt attactggtt ttgatcgaca tctttatatc
     7321 ctcagcggct catcaacact tcagcaatat gcatcacttt gactttctgc ccttcccgtt
     7381 gtaatcgccc actgatgttc agcaggcaac tcacgtcagc accaattaaa tactcagggc
     7441 ggacttccat caggtgcgca accttttctt tcaccatctc gccggatatt tcggccattt
     7501 tgaccgagaa cgtgccgcca aatccgcagc aggtatcctg ttcagcaaag gtcaacagct
     7561 ccagtccacg cacatttttc agcagcgtaa gtggttcgtc cttcaccccc agcttacggg
     7621 ccaggctaca agatgggtga tacaccgctc tcccttgcaa actggcacct acatcgacta
     7681 cccctaattt attaacaata aaagaggtga gatcctgcat acgcgcggca accttttcgg
     7741 ctcgtgatgc ccattcaggt tcatccgcca gatacgtcgg gtaacttttt acggcatagg
     7801 tgcaagagcc agctggtgaa ataatgggat cgtcgttatc ctccagtgcg gcgatcagat
     7861 ttttcatccc tggaatcgct tctttgatat aaccgctatt gatcgcaggc tgaccgcagc
     7921 atccctgttt ctccgggaaa tttacgcgac agccgagttt ttccagtagc agcacggagt
     7981 ctcgtgccat tcttgatttc agggcgtcac caatacaggt gacaaagaaa ttgacattca
     8041 caactaatac tccattactt catgcccatt tatgcccgat gtttcccccc caccggggga
     8101 tcaatcatca ataaatatgg cgttcttttt taattggcta gcaacatacc acccctcatt
     8161 tgtatgacaa atgataatct ttcatccaaa cttcaaatgc aataactaat tttatttctt
     8221 tcggcatcgc tcacatttta tggggaaatc gacgatcatt tttggcataa gaaatccatt
     8281 taaaaatggc cacatccctt atacctattc agagctactt attcaggggt tattgagata
     8341 tacactgatg gtgtacaaac aaaatcaccg acaataatca cgtatcaagt aatagtcgga
     8401 gttttattta attgaagcaa tttagtttgc atttttattt aaaaaccgag ctagagtcca
     8461 tcagaaaaaa aatatataaa gcccaaacca ccgttggaaa tagaaatagc tgaaaggctc
     8521 acaacacccg gaatgcggat tatctttgct ccgattcctg gcggttttat tcagggtgca
     8581 agctgtctga ccctttcccg atattctccc ggggtacaac caaactcgcg gacaaacgcc
     8641 ttgtgaaaag atgattcact ggcatagcct actgactcag cgatcaccac aacagggagc
     8701 gtttcccggg aaaacatctg ggccgctatt tgtagacgca actttgttaa tacagccagc
     8761 ggcgtggttc cggaaacatc acggaaaagc tgggcaaaac ttgcccggga catgtgggcg
     8821 atgctggcca gcgattcgac ggtccaggcg tgtcctggca tttccaacat ttgctgtatt
     8881 accgcaccaa gacgtggatg cagaagcaaa ctgagaatgt ttttctcagt attaacctgt
     8941 gcaatccact cacgcaccgc gagggtaaag aatgtagcgc agatctggct acacagtgca
     9001 tccactcccg gcattgccaa tctggattcc tgttgtaaaa acgggatcgc ctccctcagc
     9061 cagttatatt ctacgctgtg gttaaccggt gctaaaaaca gcgtttccgg caaagacgtt
     9121 aaaaaataac gcgccgaatg ttgcaaccga agagtgccgc agacaataca ggtcgattca
     9181 ttatcgacat gactcaggcg atgagctgaa ttttgtggta gcaggaccac atttcccggg
     9241 cgtaatgtaa aaatctctcc cgtcggcatt tccagcttcg ccgctccttg cgttaacgca
     9301 tgccaacgaa taaccgataa ttccccggca ccatgtggaa gctgccagtc acttcctaac
     9361 acgcaattct tatcgatcgt tccttgtgga gcgttaagca tcaacaaacg gctaagggca
     9421 tccatatcag actcctgagt ggtttttgat tacaaacacc aacaatctta gtaaataact
     9481 aatctaatac catcaaatag ttaatcatga tttttgtttt gtgattctga ttaagaaaaa
     9541 gaaaatacca gacgataaga caaaatatca agcgttgtga agaaatggta tttgctcttt
     9601 tgtgtctata gtcatgatgt caaatgaacg cgtttcgaca ggaaatcatc atgaataaat
     9661 atcaggcagt gattattggt tttggcaagg ctggaaaaac attagccgtc acgctggcaa
     9721 aagcaggttg gcgagtggct ctcatcgaac aatcaaatgc aatgtatggc gggacctgta
     9781 ttaatatcgg ctgcatccca accaaaacat tggttcatga cgcacagcag cacacagatt
     9841 ttgtccgtgc catacagcgt aaaaatgaag tggttaattt tttacgtaat aagaattttc
     9901 ataatcttgc ggatatgccc aatatcgacg tgatcgacgg ccaggcggag tttatcaata
     9961 atcatagcct gcgtgttcat cggcctgagg gaaatctgga aattcatggc gagaaaattt
    10021 ttattaatac cggtgcacaa accgtggttc cgccaattcc tggaattacc accacgcctg
    10081 gtgtatatga cagcaccgga ttacttaatc taaaagaatt gcctgggcat ttaggtattt
    10141 tgggcggcgg atatattggc gttgagttcg cctctatgtt cgctaatttt ggcagcaagg
    10201 taaccatttt agaagcagct tcgctgtttt tgcctcggga agatcgggat attgctgata
    10261 atatcgcgac gattttacgc gatcagggcg tcgatattat cctcaatgcc catgtggagc
    10321 gaatcagtca ccatgaaaat caagtgcaag tgcatagcga gcacgcccaa ctggcggtgg
    10381 atgcactgtt aatagcttcc ggtcgtcaac cggctaccgc ttcgttacat ccagaaaatg
    10441 ccggtatcgc agtaaacgag cgcggggcaa ttgtcgttga caagcaatta cataccaccg
    10501 cagacaatat ttgggcgatt ggagatgtta ccggcgggct gcaatttact tatatttcac
    10561 tggatgatta ccgcattgtg cgtgatgagt tgctgggaga aggcagacgt agtactgatg
    10621 atcggaaaaa tgtgccttat tccgtattca tgacaccgcc actgtccagg gttggtatga
    10681 cagaagaaca ggccagagag agtggtgctg atattcaggt ggtgacattg cctgtagctg
    10741 caattccgcg tgccagagtg atgaatgata ctcgtggggt attaaaagcg attgttgata
    10801 ataaaaccca acgtatatta ggggcatcac tgctgtgtgt tgactcccac gagatgatca
    10861 atatagtgaa aatggtgatg gatgccgggc tgccttatag catattacgc gatcagatat
    10921 ttactcatcc gtcgatgagc gaatcactca atgatctatt ttcattagtc aaataaactc
    10981 aaaatcagac gccagaacaa atattctggc gtctcagaga aaagaatctt attaattccc
    11041 tgttactcta taact
//
LOCUS       DAPHDW010000144        11052 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.146, whole genome shotgun sequence.
ACCESSION   DAPHDW010000144 DAPHDW010000000
VERSION     DAPHDW010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 11052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11052
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.146"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            109..723
                     /gene="evgA"
                     /locus_tag="RH057_003719"
     CDS             109..723
                     /gene="evgA"
                     /locus_tag="RH057_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HDV3130027.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            728..4321
                     /gene="evgS"
                     /locus_tag="RH057_003720"
     CDS             728..4321
                     /gene="evgS"
                     /locus_tag="RH057_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HDV3130028.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYTEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALEEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGQNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPDLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLNPGGWDIIPGAIYSEDRENNVSFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKVKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLV
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEHPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFQALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPVSDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(4377..5522)
                     /gene="yfdE"
                     /locus_tag="RH057_003721"
     CDS             complement(4377..5522)
                     /gene="yfdE"
                     /locus_tag="RH057_003721"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416872.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HDV3130029.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLCNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     PGTMEKLGFSWEMLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFNTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLARIHEVGVPVAPLLSVAEAIKLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(5596..6540)
                     /gene="yfdV"
                     /locus_tag="RH057_003722"
     CDS             complement(5596..6540)
                     /gene="yfdV"
                     /locus_tag="RH057_003722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HDV3130030.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(6610..8304)
                     /gene="oxc"
                     /locus_tag="RH057_003723"
     CDS             complement(6610..8304)
                     /gene="oxc"
                     /locus_tag="RH057_003723"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HDV3130031.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPMIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESTQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(8358..9608)
                     /gene="frc"
                     /locus_tag="RH057_003724"
     CDS             complement(8358..9608)
                     /gene="frc"
                     /locus_tag="RH057_003724"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HDV3130032.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(10121..10753)
                     /locus_tag="RH057_003725"
     CDS             complement(10121..10753)
                     /locus_tag="RH057_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV3130033.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQVQQTQKTAA
                     AERISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKT
                     NLNDDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVME
                     NQYLMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
BASE COUNT         3250 a         2472 c         2270 g         3060 t
ORIGIN      
        1 ttaaaggaag ctcagatttt cttattttta ttgagaaaat gagatgacgc cttatgtctg
       61 tattactaca gggagaaggg agatgcttca ttgcaaaggg aataatctat gaacgcaata
      121 attattgatg accatcctct tgctatcgca gcaattcgta atttattgat caaaaacgat
      181 attgaaatct tagcggagtt gactgaaggg ggaagcgccg ttcagcgggt ggaaacactt
      241 aagcctgata tcgtcatcat tgatgtcgat atccccggag ttaacggtat ccaggtgtta
      301 gaaacgctga ggaagcgcca atatagcgga attattatta ttgtctccgc taaaaatgac
      361 catttttacg ggaaacattg tgctgatgct ggcgctaatg gattcgtgag taaaaaagaa
      421 ggcatgaaca atatcattgc ggctattgaa gctgcaaaaa atggctactg ctatttcccc
      481 ttctctctca accggtttgt tggaagttta acgtccgacc agcaaaaact cgactcctta
      541 tcgaaacaag aaattagtgt catgcggtat attcttgatg gcaaggataa taatgacatt
      601 gctgaaaaaa tgttcatcag caacaaaact gtcagcactt ataaaagtcg cctgatggaa
      661 aaattagaat gtaaatcact gatggatctt tacacattcg cgcaacgtaa caaaatcggc
      721 taatcacatg aagtttttac cctatttttt tcttctctgt tgtggtcttt ggtcgaccat
      781 aagtttcgca gacgaagatt acaccgaata tcgtggcatc agtagtaaca accgtgtcac
      841 acttgatcca gtacgtctga gcaacaagga attacgttgg ttagcgagca aaaaaaatct
      901 tgtgattgca gtacacaagt cccaaacggc taccttgttg cataccgatt cgcagcaaca
      961 ggttcgtggt attaatgctg attatttaaa tcttttaaaa agagcgttaa atatcaaatt
     1021 aacactccgg gaatacgcag atcatcaaaa agcaatggac gcgctggaag aaggtgaagt
     1081 cgatatagtg ttatcacatt tagttacttc gccgcctctt aataatgaca ttgctgcaac
     1141 caaaccattg ataattacct ttccggcgct ggtaaccacc cttcacgact caatgcgacc
     1201 gcttacctca ccaaaaccag taaatattgc tcgggtagca aattaccccc cagacgaggt
     1261 aattcatcaa tcgtttccaa aagcaacaat tatctctttt acaaatttat atcaggcatt
     1321 agcatccgta tcagccggac agaatgatta ctttattggt agtaacatca ttaccagcag
     1381 tatgatttcc cgctatttca ctcactcctt aaatgtagtg aaatattata actcgccgcg
     1441 tcaatataat tttttcttga ccagaaaaga atccgtcatt cttaatgaag tactcaatag
     1501 atttgttgat gctttaacaa atgaagttcg ctatgaagtt tcacaaaatt ggcttgatac
     1561 aggaaacctg gcctttctga acaaaccatt agaactcact gaacatgaaa aacagtggat
     1621 taagcaacat cccgatttaa aggtgctgga aaatccttac tctcctccct attctatgac
     1681 ggatgaaaat ggctcggttc ggggcgttat gggggatatt cttaatatta ttaccttgca
     1741 aacaggttta aatttttctc cgatcaccgt ttcacacaat atccatgccg gaacacagct
     1801 taaccccggt ggatgggata taatacctgg cgctatttat agtgaagatc gagaaaataa
     1861 tgtttcattt gcagaagctt tcataacaac gccatacgtt tttgtcatgc aaaaagcacc
     1921 tgacagtgaa caaacattaa aaaaaggaat gaaagttgcc attccatatt attatgagct
     1981 gcattcgcaa ttaaaagaga tgtatccgga ggttgaatgg atacaggtcg ataatgccag
     2041 cgctgcattt cacaaggtta aggaaggtga acttgatgct ctggtcgcga cacagttaaa
     2101 ttcgcgttac atgatcgatc attactatcc taatgaactt tatcattttc ttattcctgg
     2161 cgttccgaat gcatcgcttt cgttcgcttt tcctcgcgga gaaccggaac ttaaggatat
     2221 tattaataaa gcactgaatg caattccccc aagcgaagtt ctgcgcctga cggaaaaatg
     2281 gattaaaatg cccaatgtga ccattgacac atgggaccta tatagcgagc aattttatat
     2341 tgttacgaca ttatccgttt tattagttgg cagtagcctt ttatggggat tctacctgtt
     2401 acgctcagtt cgtcgtcgta aagtcattca gggtgattta gaaaaccaaa tatcattccg
     2461 aaaagcactc tcggattcct taccgaatcc aacttatgtt gtaaactggc aaggtaatgt
     2521 cattagtcat aatagtgctt ttgaacatta tttcactgcg gattactaca aaaatgcaat
     2581 gttaccatta gaaaacagtg actcaccctt taaagatgtt ttttctaatg cgcatgaagt
     2641 cacagcagaa acgaaagaaa atcgaacaat atacacacag gtatttgaaa ttgataatgg
     2701 catcgagaaa agatgcatta atcactggca tacattatgc aatcttcctg caagtgacaa
     2761 tgcagtatat atttgtggtt ggcaagatat tactgaaacg cgtgatctaa ttaatgcact
     2821 cgaggtagaa aaaaataaag cgataaaggc taccgtagca aaaagtcagt ttctggcaac
     2881 gatgagtcac gaaataagaa caccaataag ctctattatg ggcttcctgg aacttctgtc
     2941 gggttctggt cttagcaagg agcaacgggt ggaggcgatt tcacttgcct acgccaccgg
     3001 acaatcactc ctcggcttaa ttggtgaaat tcttgatgtc gacaaaattg aatcgggtaa
     3061 ctatcaactt caaccacaat gggtcgatat ccctacttta gtccagaaca cttgtcactc
     3121 tttcggtgcg attgctgcaa gcaaatcgat cgcattaagt tgcagcagta catttcctga
     3181 tcattacctg gttaagatcg accctcaggc gtttaagcag gtcttatcaa atttactgag
     3241 caatgctctc aaatttacca ccgagggggc agtaaaaatt acgacctccc tggttcacat
     3301 tgatgacaac cacgctgtta tcaaaatgac gattatggat tctggaagtg gattatcgca
     3361 ggaagaacaa caacaactct ttaaacgcta cagccaaaca agtgcaggtc gccagcaaac
     3421 aggatctggc ttaggcttaa tgatctgcaa agaattaatt aaaaatatgc agggcgattt
     3481 gtcattagaa agtcatccag gcataggaac aacatttacg atcacaatcc cggtagaaat
     3541 tagccagcaa gtggcgactg tcgaggcaaa agcagaacac cccatcacac tacctgaaaa
     3601 gttgagcata ttaatcgcgg atgatcatcc gaccaacagg ctattactca aacgccagct
     3661 aaatctatta ggatatgatg ttgatgaagc cactgatggt gtgcaagcgc tacacaaagt
     3721 cagtatgcaa cattatgatc tgcttattac tgacgttaat atgccgaata tggatggttt
     3781 tgagttgact cgcaaactcc gtgagcaaaa ttcttcctta cccatctggg ggcttacagc
     3841 caacgcacag gctaacgaac gtgaaaaagg gttaagttgc ggcatgaact tatgtttgtt
     3901 caaaccgttg accctggatg tactgaaaac acatttaagt cagttacacc aggttgcgca
     3961 tattgcacct cagtatcgcc accttgatat cgaagccctg aaaaataata cggcgaacga
     4021 tctacaactg atgcaggaga ttctcatgac tttccagcat gaaacgcata aagatctacc
     4081 cgctgcgttt caagcactag aagctggcga taacagaact ttccatcagt gtattcatcg
     4141 catccacggt gcggctaaca tcctgaattt gcaaaagttg attaatatta gccatcagtt
     4201 agaaataaca cctgtttcag atgacagtaa gcctgaaatt cttcagttgc tgaactctgt
     4261 aaaagaacac attgcagagc tggaccagga gattgctgtt ttctgtcaga aaaatgacta
     4321 aatagcggct cccacaatgt tcaaatgtgg gagctattta ccagcacatc ttacatttat
     4381 gatgagaact cctggcgaat ttgttcccca tgctggtcga gcgttgccgc tcccggcata
     4441 acatgcgggt ccgcgcagcc gctgattttt atcggattac ccggcatcat tattcccccg
     4501 gcttcaatca acatatttct cgcctgagtt tgtggcaatt taatggcctc agccacactt
     4561 aataacggcg cgacgggtac accaacttca tgtattctgg ctaaccaaac ttcagctgcc
     4621 tgcgttttta acgtccgctc aatatattgt ttaagaatag cctggttttg tacgcgtaaa
     4681 atattgctgc taaatcgggg atcattaacc agttccgtaa gctccagtgc ctggcataac
     4741 gcagaaaaaa gcttgtcatt accacaacaa atcgtaatcg gcttatcctg agtattgaaa
     4801 acatcaaaag gtgccatgta gggatggcga tttcccagac gttgtggtga cttccctgtc
     4861 gcgatatatg ccatcagacc atgctccaga aaactcagcg tggcatcaaa catcgctata
     4921 tcgacatgcg cccctctctg gctcttttcg cggccataaa gtgcactcac tattccgctg
     4981 aataaataga caccgccgca tagatccgca agagatgtac caacgcgcac tggcggagca
     5041 tcaggatatc ctgtttccat cattatcccg ctcattgcct gaatgatggt atcgtaggca
     5101 ggagcatctt ttagcggacc ggtatgtccg aaacctgacg atgaagcata tatgaggcgc
     5161 gggttgattt cttgaagcat ttcccatgaa aaccccagtt tttccattgt acctgggcga
     5221 aaattctcag ctaatacatc agcttgtttg agcatattta taaatatact tttatcgtga
     5281 tcattcttta aatcaagaac cacactctct ttgccatgat taataaaact gtaatagagt
     5341 gactgtccat ccacataggg accaaatgtg cgggtatcat caccatgacc tggtggctca
     5401 actttaatta cccttgcgcc catattacaa agaagttgag ttccgaaagg tccattaagg
     5461 acatgtgtca tatcgataac taataagcct tcaaacggcc ctttgctttc attatttgtc
     5521 atttgcttat gccttgaaaa aagccgaagg tgaaaagaca cttcggcatt tatgtaaaat
     5581 caataacatg attaattatg aaaccagtcg actgacataa atccacaagg gagccgtgac
     5641 gacaaaaccc agtacgctca cagccaatga cgctgtacca gtgcgggtgt agacattaaa
     5701 ccggctggca ataatgattc cggagaatgc cggcggtaat gcgcctgcca gtaccatcat
     5761 ctgcaaatgt tcgctgttca aatgacatgc cataccaacg agaagcagcg ccagtggcat
     5821 cagaatcagc ttcaggaagg tgttataagc aatttcggca ctgaactcga atttatgtgc
     5881 agccagagtc aaccccgcag cgaataccgc tacccctgag ttagccttcg caatcagatt
     5941 aaaggttggg tcccatgctg ccggaatttt taccccaacc aacaccagga tcgttgccag
     6001 aacaggtgcc catacaactg gctcctttgc cgcagaaatt aatgcgctca gattactatt
     6061 cttcttacca tccgctcctg aggaaggatt cagcaaatac agaccaatag gaatagtaat
     6121 tgcgttaaca ataatagaaa taattgctac cactaaacct gttgatacgg aatcaccata
     6181 aataggatcg agaactgcaa accccaggaa tccaatggta ggtgaacctg caattaatgc
     6241 acatacagct gcttctgcat gggtacgttt aaaaaatttg tagcaaccga accaggagaa
     6301 aaagaaacat ccgacaataa ccacaagtga tacaagggtc agacgagtgt ccgcaaaaat
     6361 catttccctg tttgcccgag taatagatac aaatagagcc gcaggaagcg cgtagtttaa
     6421 taccagttta ttaaatgccc gagcttgatc ttctgaaaaa gtttctcgtc tgccgctaaa
     6481 ataacccaac agcatgatta cgataatagg caataaatcg ccaataaaaa atgttagcat
     6541 aatagtattt ccgataaggg tcccctcacc atcgcataat gagttggcaa gaggagacga
     6601 tgtcagtatt taattaccag cgacttgttt tgggttaagt ttggtaatat ggccactttc
     6661 agttcctgct gcagggtcga tgaccacatt aataatggtc ggtttgcgcg actgaatacc
     6721 ggtggttaaa gcatgacgaa gttcatctgt cgtggtgacg ttatagccaa cgccacgaaa
     6781 cgcatccatt aatttgtcat accttgcatg gtgcaacaga tccgttggtg atggtgcacc
     6841 agcgccactg agatcaacac cgtctcctct gtagatgccg ccattattaa aaataacgat
     6901 cgtcaccggc aggttatatc gacaaatcgt ttcaatttcc atcccactga aaccaaaagc
     6961 actatcacct tcaatggcga caaccggaga accagaagtc acgctagcac cgatggcata
     7021 gcccataccg atgcccatga caccccaggt accacaatcc agacgacgac gtggtttata
     7081 catatcaata atatttcgtg cattatccag ggtatttgca ccttcattaa ctaaataaat
     7141 atcctggttc tcgcgcaata cgtcgcgcac agcacttaat gcattaaagt aatttaatgg
     7201 ttgagtatct gtacttaatt tttcatgcat tttttgtgca ttttgctgct tgtggatatt
     7261 taaaatatcg cgccatacca gtggagtcgt aaatgtgttt tgtttcagtt ctgccagcat
     7321 accttgcata ctggatgcaa tatcaccaac gactggcaca gcaatggggc ggttgctgtc
     7381 aatttcctgc ggttcaatat ccagttgaat aaactgtgta tctgccgccc atcctttttt
     7441 accgtgtgcc aataaccaat tcagtcgtgc accaacaagc atgacaacgt cagcatttgc
     7501 cagggcaaac gaacgcgcag ctgccgcaga aagtggatgc gtatcttcaa ggatcccttt
     7561 cgccatagac attggcagga atggaatctg agtactttca ataaattcac gaagctgttc
     7621 atcagcttgt gaatacgccg cgcctttgcc aaggataatt aatggccgtt cagcttttgc
     7681 taaaagcgaa attgcgctag tgactgactt cgggcatggc aataatgctg gcgatggatt
     7741 ttcaacttta acaatcgtgg ttaacgcttc gtctttttcc atcgtcgcgg ccaggacatt
     7801 tgctggcaaa tcaagataaa ctccgccagg gcgacccgat acagagaccc ggatagcgcg
     7861 tgccaatgca atgccaagat cctgcggctg attaacgcga aatgctgctt tggcatacgg
     7921 ttttgccgca ttcatttggt ccagctcttc ataatcacct tgctgtaggt cgacgatcgc
     7981 gcggtcgctg gagccgctaa tcatgatcat cggaaaacca tttaccgttg cgttggccaa
     8041 tgcggtcaaa ccattgagga atcctggcgc agaaactgtc aggcagatcc ccggtttttg
     8101 ggtaagaaaa ccgcttgccg cagcggcata gcctgccgac tgctcatgac gaaaaccaat
     8161 ataacgaatg ccttccgcct gggcatggcg tgccatatcc gtcacaggaa tacctacaac
     8221 accataaata gtgtcaatat tattctgttt taatgcttca acgatgatat gcataccatc
     8281 tgtcatttga agttgatctg acatgattaa taacctcgaa atttttataa attgggagcc
     8341 agaagccccc gttaatatca gatggcgtgg ttttgcttca ttgcagcaat ttcatcgtcg
     8401 ctataaccca gctcctgcaa tacagcagcg gtatgttcac ctaatagcgg cgcagcttta
     8461 atatccggcg taaaggcaga gaatttcatt ggacaaccaa cggtcagata ttttccacgc
     8521 aacggttgtt ccacttcaac aacactgcca ctttggcgca aagagggatc aagtgaaatt
     8581 tctttcatac ttaaaaccgg tgcacaagga atatcaaact gagtcaaata ggccaccgct
     8641 tcatgtttat caatagtgac agtgtatttt tcgatttcag caaaaatatc gaaaatatgt
     8701 ggctgtcgtg catgggctgt actgtatgcc ggatcggtaa tccattctgg tttgccgatg
     8761 gctttacagg tgttttccca gttttgctcc tgaatagtga aataaatata ggcgttagga
     8821 tcggtttccc agcctttaca tttcaggatc cagccaggct gaccgccacc acctgcatta
     8881 ccaccgcggg gaactgcatc accaaatgta ccattcggat actgcgggta ttcttccaga
     8941 taacccaatt tatcgagacg ctgctggtca cgtaatttca cgcggcaaag gttcaatacg
     9001 gcatcctgca ttgacatggt gactcgttgc ccacgccccg ttttttcgcg atgcagcaaa
     9061 gcagcaagta aaccgatcag caaatgcatt ccggtgttgc tgtcacccaa cgctgcagcg
     9121 cttaccagcg gcggaccatc ccaaaaaccc gtagtggatg ccgcgccacc cgctgcctga
     9181 gcaacgtttt cataggcttt tacattcaca taaggcgaac actcatcaaa ccctttgatc
     9241 gaaccaaaaa tcagacgtgg attgatttct tgaatatgct cccaggtgaa gcccatgtga
     9301 tcaatggccc ctggatgaaa gttctcgact aagatatcag cttcgcggat cagcttttcc
     9361 attacctctt tgccttccgc tgttttggta tttaactcaa tagaacgttt gttactgtta
     9421 agcatggtga agtaaagcgc atcgatatca ggaatatctc gcagctggtg acgcgttacg
     9481 tcaccaacgc cgggacgttc aattttaata acgtcagcgc caaaccaggc cagcatttga
     9541 gtacaagatg ggccagattg cacaccggtg aaatcgagaa ctttaattcc ttgaagtgga
     9601 gttgacatag caatacctat tagttaatta aatatgtgtt aatttagcgt ttatttaagg
     9661 cgtaatatct tctataaata acccgcaaaa tatcgtcgta gctgcactcg atatttttaa
     9721 aatcctgtat caagtgctga aaatcatttc atcgccactt ccaattcctt tctgcatggt
     9781 aagtagtgag gacgaaaaga agaatatgat aaatattgat tcgtggtaat cagagagagt
     9841 cgcgacattt gtgatgttat tcttacaact gtaagaagcg ccggtgattt acaagtaagg
     9901 gtatgattac gctttttaaa gcgtaccata aaacataaca taataaatga tttgtgatga
     9961 cacgggatta ttgggattta ctttgaggaa gataaaatgc gtgtttatag aaccgcgagc
    10021 gccaaataat tttatgacca caacctgtcg tattgtggtc ataaaaaatt gtcggctgga
    10081 gggagttaaa tcctttacct caatatactg cctgtcagca ttagttcaca aacagtgctt
    10141 cgctgtcgac gatgattcca tcttccgcac ctttaagcgc caggtttgcc tcgtagtatt
    10201 gctttttatc aagcagtttc tgcgcatcag caagtgcatt acgagtctgt ttaagcggca
    10261 tcagatattg gttttccatt acgccaacac cggccaggcg aagttcttcc attgcgccct
    10321 ttttatcccc tttcgccatt ttttcattgg caatttttat cgcggcttct ttttcgggag
    10381 ttgctacata actctcagaa attccgacag atgcattaat gacaatatat tggtcatcat
    10441 tgagattggt ctttttacct ggcttagcga actttgccca ttcggtactg tcatctgaca
    10501 gcaaagcgga agcttcattg gtcagttctt ttgccttttc tggatcgcca tgaaacagag
    10561 ccaggcgcgc tacctgcacg tcacgcatcg catataaacc ttgttcagag atacgctctg
    10621 ctgctgccgt tttctgggtt tgctgaactt gctgagtttg ctgttgtgcc gctaccggtg
    10681 cattatcagc agcccataca gttgaagatg ccagaattgc tgtgaccatc gtagccataa
    10741 ttaaacgttt catacttacc tcattcttta acagttttag aaaacgccgc catcatattg
    10801 ggtgagcatg aaatgagaaa tcagagagca acggtcattt tgaaagggtt atcttacagt
    10861 tgtagttatt atcgcaagaa gtattttagt cttacaattg tagttattat cgcaagaagt
    10921 attttagtct tacaattgta ggaatctgct tcctgtcgca gagaactatt cttatgtacc
    10981 acaaagagat gtcatttatt atatcgatgt aaatattgat taattatatg taaatacagc
    11041 cataacatcg ta
//
LOCUS       DAPHDW010000145        10951 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.147, whole genome shotgun sequence.
ACCESSION   DAPHDW010000145 DAPHDW010000000
VERSION     DAPHDW010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.147"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            153..362
                     /locus_tag="RH057_003726"
     CDS             153..362
                     /locus_tag="RH057_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130034.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVAS"
     gene            479..2800
                     /locus_tag="RH057_003727"
     CDS             479..2800
                     /locus_tag="RH057_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3130035.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSARACVSSLDEESTTDTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAATAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKNETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDVN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELGDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRL
                     EAGSYVANLAAANTLFVMRLNDRAGEMRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGAELLTGGFTDSDSWRLGVMAGYARDYNSTHSSVSDYRSKGSVRG
                     YCAGLYAIWFADDISKKGAYIDAWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGASNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(2857..3192)
                     /locus_tag="RH057_003728"
     CDS             complement(2857..3192)
                     /locus_tag="RH057_003728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HDV3130036.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(3196..3540)
                     /locus_tag="RH057_003729"
     CDS             complement(3196..3540)
                     /locus_tag="RH057_003729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HDV3130037.1"
                     /translation="MKKKILAFGLISALFCSTPAMADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVGAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            complement(3542..3715)
                     /locus_tag="RH057_003730"
     CDS             complement(3542..3715)
                     /locus_tag="RH057_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130038.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3816..4001
                     /locus_tag="RH057_003731"
     CDS             3816..4001
                     /locus_tag="RH057_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130039.1"
                     /translation="MSRRYSSATITLVILFCRRPHCRKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3962..4081
                     /locus_tag="RH057_003732"
     CDS             3962..4081
                     /locus_tag="RH057_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HDV3130040.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            4116..4460
                     /locus_tag="RH057_003733"
                     /pseudo
     CDS             4116..4460
                     /locus_tag="RH057_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(4832..5098)
                     /gene="minE"
                     /locus_tag="RH057_003734"
     CDS             complement(4832..5098)
                     /gene="minE"
                     /locus_tag="RH057_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HDV3130041.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(5102..5914)
                     /gene="minD"
                     /locus_tag="RH057_003735"
     CDS             complement(5102..5914)
                     /gene="minD"
                     /locus_tag="RH057_003735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HDV3130042.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            complement(5938..6633)
                     /gene="minC"
                     /locus_tag="RH057_003736"
     CDS             complement(5938..6633)
                     /gene="minC"
                     /locus_tag="RH057_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HDV3130043.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALEDKIAQAPAFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTTPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            7153..7521
                     /locus_tag="RH057_003737"
     CDS             7153..7521
                     /locus_tag="RH057_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130044.1"
                     /translation="MNMMRIFYIGLSGVGMMFSSMASGHDAGGLQSPACGVVCDPYIC
                     VNSDGISPELTRKYLGEKAAENLQSLQGYDPSEFTFANGVFCDVKEKLCRDDRYFGVD
                     GKRSGKINQITTKMLFMCRE"
     gene            complement(7641..8042)
                     /locus_tag="RH057_003738"
     CDS             complement(7641..8042)
                     /locus_tag="RH057_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing protein"
                     /protein_id="HDV3130045.1"
                     /translation="MKIKSISKAVLLLALLTSTSFAAGKNVNVEFSKGHSSAQYSGEI
                     KGYDYDTYTFYAKKGQKVHVSISNEGADTYLFGPGIDDSVDLSRYSPELDSHGQYSLP
                     ASGKYELRVLQTRNDARKNKTKKYNVDIQIK"
     gene            8283..8576
                     /locus_tag="RH057_003739"
     CDS             8283..8576
                     /locus_tag="RH057_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HDV3130046.1"
                     /translation="MFCVIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            8648..9307
                     /locus_tag="RH057_003740"
     CDS             8648..9307
                     /locus_tag="RH057_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HDV3130047.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEQRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLATRV
                     L"
     gene            9399..9845
                     /locus_tag="RH057_003741"
     CDS             9399..9845
                     /locus_tag="RH057_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HDV3130048.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(10052..>10951)
                     /gene="hlyE"
                     /locus_tag="RH057_003742"
     CDS             complement(10052..>10951)
                     /gene="hlyE"
                     /locus_tag="RH057_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
                     /protein_id="HDV3130049.1"
                     /translation="VADKTVEVVKNAIETADGALDLYNKYLDQVIPWQTFDETIKELS
                     RFKQEYSQAASVLVGDIKTLLMDSQDKYFEATQTVYEWCGVATQLLAAYILLFDEYNE
                     KKASAQKDILIKVLDDGITKLNEAQKSLLVSSQSFNNASGKLLALDSQLTNDFSEKSS
                     YFQSQVDKIRKEAYAGAAAGVVAGPFGLIISYSIAAGVVEGKLIPELKNKLKSVQSFF
                     TTLSNTVKQANKDIDAAKLKLTTEIAAIGEIKTETETTRFYVDYDDLMLSLLKEAAKK
                     MINTCNEYQKRHGKKTLFEVPEV"
BASE COUNT         2884 a         2398 c         2601 g         3068 t
ORIGIN      
        1 gccgacttat ttatcattta tatattgtcg ctgtttatct atttaaattt tttgtgcttt
       61 tgtttttgtc tttaccctcc gttttggtaa tctgagctca taggttattt gaccaggaat
      121 gtttgttaat ccaatgaatg gatggttaat ctatgaaact gaaaaaactc cccgggttta
      181 gtttgggact tattgctctg gcggtgggta atgcatatgc tgaacagttt attcagcacg
      241 atgaatataa acatgctgtg gcaataaaag atggaaaagt aaaagatatt gataatgcgc
      301 tagttataac aacgggtaat ggaagttacg ggtatccgtt gcaggagagg gtagcaagtt
      361 aaccattaac aatggcgtca ttgtaacaac gggcggttta tatccttctg acaatagtcc
      421 tctaagcaca gcattaacag ctagcgcaat cgtaagtgaa tttggcggtg ctgttatttt
      481 gcagggtaaa aacacgattg tcacaacggg ggattactca attggtttgc taagtcaaac
      541 gagtggaaac ctgaatactg acaccataat aagagtcaac tctgacggtt cggttacccc
      601 ctctttttct gacggagatg atacatttat tgttactgcg ggtaatcacg cagtcggcgt
      661 tcttgcgtgt gcatctcccg gaagcgcgcg tgcgtgtgta tcttctcttg atgaagaaag
      721 tactaccgat acaggaagta atgaaaataa tgccatagca aaactggata tggcaaaagg
      781 ggagatcaca acccacggaa cagaaagtta tgccgcgtac gctaatggca ctgtcgtcaa
      841 ggctggtgac acgcttgatt ataccaatgc cagcgttaca ttaaccgatg ttgatatcac
      901 cacgcatggg gacaatgctc atgccatcgc tgcccgccag gggacagttt catttaacca
      961 gggagaaatt tatacaacgg gtcctgacgc cgcgactgct aaaatttata atggcggcac
     1021 ggtgacgctg aaaaatacat ctgcagtcgc gcatcagggg tctggaattg tgctggagtc
     1081 ttccataaat ggtcaggaag caacggtaga tattttatct ggtagctcac tacggtcagc
     1141 aaatgaaatc ctctaccata aaaatgagac gagtaacgtg accattacgg atagtgaggt
     1201 atcatcggct gcagatgttt ttatcaataa tattaaaggt catttgaccg tcgatgcaac
     1261 taattcaaaa ataacgggtt cagctaatat ttcaacagat gttaacactc atacctatct
     1321 gtcgctttca gataatagta cctgggatat taaagctgac tcaacggtga gcaacctcac
     1381 ggttgataat agtacggtct atatttcccg ggcagatgga cgggatgtcg aaccaacaag
     1441 attaacaata actgaaaatt acgttggtaa taatggcgta ttgcatctca gaactgaatt
     1501 gggtgatgat aattcagcta cggataaagt cgttattaat ggaaatacct ctggaacaac
     1561 ccgagttaaa gttaccaatg caggaggcag cggagcttac acgttaaatg ggatagagat
     1621 tatcagcgtt gagggggaat caaatgggga atttattaag gattcgagga ttttcgccgg
     1681 tgcctacgaa tattcattaa cccgaggtaa taccgaagcg accaataaaa actggtatct
     1741 gactaacttc caggcaacga gcggcggtga aacaaactcc ggaggaagtt cagcgcctac
     1801 tgttgcgcct acccccgtcc tgcgcctcga agctggaagt tacgtcgcca acctggcagc
     1861 cgctaacact ctttttgtta tgcgtctgaa cgaccgtgcg ggtgaaatgc gctacatcga
     1921 tcctgtaact gaacaggagc gttcaagccg actttggcta cgtcaaattg gcgggcataa
     1981 tgcctggcgt gacagcaacg gacagttgag aacgacctcg catcgctacg tctcgcagtt
     2041 aggggccgag ctgttaaccg gtggttttac cgacagtgac agttggcgtt tgggagtgat
     2101 ggctggttat gcccgcgact acaactcaac tcattccagc gtgtcggatt atcgttcgaa
     2161 agggagtgtc agaggctatt gcgcagggct gtatgccatt tggtttgccg atgacatcag
     2221 taaaaaaggc gcatacattg acgcctgggc gcaatatagc tggtttaaaa actcggtgaa
     2281 aggggatgaa ttagcctatg aatcctatag tgcgaaaggc gcaaccgtct cgctggaagc
     2341 gggttacggc tttgccctga ataaatcctt tggtctggaa gcggcgaaat acacgtggat
     2401 attccagcca caggcacagg ctatctggat gggcgtcgat cataatgcgc acaccgaagc
     2461 caatggctca cgtattgaga atgacgcaaa taacaacatc cagacccgac ttggtttccg
     2521 cacctttatt cgtactcagg agaaaaacag cggtccgcac ggtgacgact ttgaaccttt
     2581 tgttgaaatg aactggatcc ataacagtaa agattttgct gtctcaatga acggtgtgaa
     2641 agtcgaacaa gatggggcga gtaatttggg ggaaattaaa cttggcgtaa atggcaacct
     2701 gaatccagcg gccagcgtct gggggaatgt gggcgtgcag ctgggtgata atggctacaa
     2761 tgacaccgca gtgatggtgg gcctgaaata taagttctga tcccgccgtt agctaaaaaa
     2821 ccgcgtcgta ttcatcgacg cggtacatat gaaatattat tttttgccga tagcacgcat
     2881 ggtgtcatca attgccgtga tcaacagcat ttgcgggtct ttagcgcaaa cctgattcag
     2941 tttttctacc actttggcgc tcagttccgg agattgctca atttttaaat cacggctggc
     3001 aacgctggca ttacccatta ccgcaacaat ttctgcaacc tgtgcgctgt cagtttttgc
     3061 catttcgttg gcttctgcgc aagtaatata ggtttctgac ggcaaaccgt ttttaatatt
     3121 gtagtcctgc gcccaggtca ttggtgcgaa aacaaacagg cccgccagta aagcaaattt
     3181 tttcatcatc attccttatt tcattttacc cagaattgca ccacccgtac cgccaatcac
     3241 ggcaccttta atcgcccctt cgaggccatt gccggtcaga acgccagtga cagcaccaac
     3301 ggcggcacct acttttgcac ctttacgcgc atttttaccg tcgcggcctt tttctgttac
     3361 tgcaccaaca ccagcgccaa cagctgcacc tttcagtacg ccattaacac cattgccagt
     3421 aagtaaacca acgcctgcgc ctagcaatgc acctttcgtg gtgcgattca tatccgccat
     3481 cgctggcgtg gagcagaaca atgctgagat aagcccgaag gcaagtattt ttttcttcaa
     3541 cttagatgtc cggtattaag taagttgcac acacaataat ttcgtcttca attaagatct
     3601 gcttaactaa agaacgctcg ctattattca gataattcaa aatgagcgtg gctgtgatga
     3661 taggaattat gttttttacg tgaatgagaa taatcttaaa tgaggaataa ctcattgatt
     3721 gacaatattt ttattcaaga agtgtcattg actgttaacg caatgttgta aaggtaagat
     3781 aatctgattt atcaatatta ttgtgtgatt tttatgtgag cagaagatat tcatcagcaa
     3841 cgattacatt agtcatttta ttttgccgac ggcctcattg tcgaaagata agcgtacgac
     3901 agtattatct gaaaagagtg attttttatc caactacact tcagcgcact gcgtgtaaaa
     3961 aatgcctctt tcttatgcgg gatatcatca tttcatcatg atgtctttgg tgagcggtga
     4021 acacaataca cctgcgctgt ctcttcagga tgaatcccct ggtctggtgc ctgcgggctg
     4081 atgttgcagc agagcttagg tcacttagac gctactatca tttatccaat ggcatggaat
     4141 cgaaatcagt cgatacccgc agtatatatc gtgaactggg tgcaacgctg agttacaaca
     4201 tgcgtctggg gaacggtatg gaaattgaac cctgcctgaa ggcggctgtg cgcaaagaat
     4261 ttgtcgatga taatcgggtg aaagtgaata gtgacggtaa ttttgtcaat gatttgtcgg
     4321 gcagacgtgg aatataccag gcaggtatta aagcctcatt cagcagtacg ttaagcgggc
     4381 atttcggggt ggggtatagc catggtgccg gtgtggagtc cccgtggaac gcggtggctg
     4441 gtgtgaactg gtcgttctga ccatcaacga ttaaactgcg cttcggcgca gttttcgttt
     4501 acaggatgtt gaaagagaaa attctggggc aaaaaaagcc cgccagttac ggcgggaaac
     4561 ctcatcctat gggagaacaa tgaataatga aattgcgggg ttatcatctc ccagtatatc
     4621 cacactaaca acatggttat ttactcaacc aggcataaac attttgttct gtgcgtggga
     4681 acagccttaa ggtgtaaagg gggaggtgga aatagcaatg aggagtatca gcaagaatac
     4741 tcgccgcttt accacaacct ggatgagagg gatgaaaaac tcaaggcaga aatgactctg
     4801 ccttgaagat aaatgcgttt ttacagcggg cttatttcag ctcttctgct tccggtaagg
     4861 tcacgttcag ctcaagaata gaaatatcgc catctttttg ctcaagctgt acggttacca
     4921 tctcaggatc aatttgtacg tatttacaaa tgacctcaag aatatcttta cgcaactgcg
     4981 gcagataatg cggttctgca tcgctgcgac ggcgttcagc aacaataatc tgcagccgtt
     5041 cttttgcaat gttggctgtg tttttcttcc gcgagagaaa gaaatcgagt aatgccataa
     5101 cttatcctcc gaacaagcgt ttgaggaagc ctttcttctc ttcttcaatg aagcggaaag
     5161 gacgttcttc tcccaacaga cgttctacgg tatctgcgta ggctttaccc gcatcggcgt
     5221 taatgtcgag aatgaccggt tcaccctggt tagaggcgcg caatactgat tgatcctctg
     5281 ggatcacgcc gacgagtttg atgcgcagga tctccagcac atcttccatg ctcagcatgt
     5341 cacctctgct tacgcggcct gggttatagc gcgttaacag caggtgctct ttaataggct
     5401 cttcgccatt ttctgcgcgg cgtgatttcg acgccagaat gcctaaaata cggtcagagt
     5461 cgcgtactga ggagacttcc gggttggtgg taataatggc ttcgtctgca aaatagagtg
     5521 ccattaacgc accggtttca atccctgccg gggagtcaca aacgataaat tcaaaatcca
     5581 tcgctttcag atcatcaaga actttggcga ccccttcacg ggtgagggca tctttatcgc
     5641 gtgtttgcga tgccggaaga atatagagat tttcagtacg cttatcttta attaacgcct
     5701 gatttagcgt tgcatcgccc tgaatgacgt tgacgaaatc gtaaacgacc cggcgttcac
     5761 aacccataat caggtcgaga ttacgcaggc cgatatcaaa atctatcacg acagttttct
     5821 ttcccttctg ggccaaacca gtggcgatgg ccgcgctgga ggttgtctta ccaacacccc
     5881 ctttgcccga agtaacaaca ataatgcgtg ccatagaaat tccttgttaa aaagggatca
     5941 atttaacggt tgaacggtca aagcgttttc gactaactgc agtcgcgccg ctttgccata
     6001 aaattctgct gggatttgat cactcagcca gtattcacct gcgatggaca ccagttccgc
     6061 catcaggttc gtacaaaata tttgcgtttc ccggtcacca cttgcccctg caagcgcacg
     6121 accgcgcatc atgccataga catgaatgtt cccatcggca atcaattcgg ccccagcgct
     6181 aacgtggctt gtaacaatca gatcacattg tggagcataa atacgctgac cggaacgcac
     6241 cggggtatct attaaacgcg tttttgtgac cggcgttgta ttttgcgctg gagcctgtgg
     6301 tgcgggagct ggacgcggcg ctttttcctt tccttccgtc aggataggca gccccatctt
     6361 ttcaatttcg gctttaagtt gcgcatcttt gcagccactt acgccaataa cccgcaaacc
     6421 ggttgccgaa accgccttat gcatcgctga ccagtttacc gggtcttcca gtgcactgac
     6481 gttgagtaca acgggggcat gttttaaaaa tgcgggggcc tgagcgattt tgtcttccag
     6541 cgcctgatgg ataaccttag gttctgcctc atgcagatga accacagata aagtgaagct
     6601 actgccttta agctcgattg gcgtgtttga catcctggcc ttactcaatt agctattaat
     6661 catcgccagc gcgcgatgat gttccgaaga ctataaggca tgttatagtc tggattatat
     6721 tgaggcaagt caccctccca tttattcaga gtaaaagtct attctgtgat aaatggcgct
     6781 gattcatagc ttaaaaaata cccttgtcaa tcaacccatt gccgtcgtac ttttgattgt
     6841 tcttatttac gcttcctttt tccgcaccct aatcgtgaca gcatctccag acctgtttaa
     6901 atatccagta ttgaggcaag tgtatttttt caccacgcca ggctttctta actttccatt
     6961 ttttgcggca tcattaaaga acagataccc gttatcttaa tatccgccac agaattgctc
     7021 tatcatagaa accgcagaag acgtctgata cttctgtcag gtcatcgtaa ctcgtccagg
     7081 tcagcggttt tgattacgga ttgatgatga aatgagcata agtgttgtac ggttggatta
     7141 tcaggatgtg ctatgaatat gatgcgtatt ttttatatcg gattgtcagg tgtggggatg
     7201 atgttctcat caatggcgtc tggacatgac gctggcggac ttcaatctcc ggcgtgcggc
     7261 gttgtttgcg acccgtatat ttgtgtgaac tcagatggca tttccccaga gttaacaagg
     7321 aaatatctcg gcgaaaaagc cgctgaaaac ttacaatcat tacaaggcta cgatcccagc
     7381 gaatttacat tcgctaacgg tgtattttgc gatgttaaag aaaaattatg tcgtgatgat
     7441 cgttattttg gtgtggatgg taagcggagt ggaaaaatca atcaaatcac cacaaagatg
     7501 ttatttatgt gtcgtgagtg atgccttgtt tacattatgt tctattcggc attgtctgct
     7561 attcgtcatt aacctcttga gcgacagaga ggagtggggc gttaacgttt atgacgggga
     7621 gagtccccgt cggttgacat ttacttaatt tgaatatcga cgttatattt tttagttttg
     7681 tttttacggg cgtcgtttcg ggtttgcagc actcgcagct catatttgcc agaagcaggg
     7741 agtgagtatt gtccgtgact atcgagttct ggtgaatatc tggataaatc aactgaatca
     7801 tcgattcctg gcccgaagag gtaagtatca gcgccttcat tagagatact cacatgcact
     7861 ttctgacctt ttttggcata gaaggtatat gtatcgtaat cgtatccctt tatttcgccg
     7921 gagtactgag cgctgctatg gcctttgctg aactcaacat tgacattctt acccgcagca
     7981 aagctcgtgg aggttagcag tgcaagcagc aatacagcct tactgatgct cttgattttc
     8041 attggtagtc tcctgttcag aatgagtacg atgaactgtt ataatataac aatcccttac
     8101 gggagaattc cagtggcttt tctgcgttcg gtaaaatccc ggcgcggcat tttttgccgg
     8161 ggggattact tataaagggg catggactca acaaaccgta gaaatctaca gctggctggc
     8221 ttgtggcgcg ggttcattga tgttagctta tgccaaaacc tggcatctta aagagtaaaa
     8281 gtatgttttg tgtgatttat cgaagcagca agcgtgacca gacctattta tatgtcgaaa
     8341 aaaaagacga tttttcgcgt gttcctgagg aactgatgaa aggttttggt cagcctcagt
     8401 tagcgatgat tctgccgttg gatgggcgga agaaactggt caatgctgat attgaaaagg
     8461 ttaaactggc attaaccgag caaggttact atttgcagtt accgccacca cccgaagatt
     8521 tgctgaagca acatctttcc gtcatggggc agaaaacaga cgacactaac aaataaccga
     8581 tatccggcgg tggcattatc tttgtcggcg cgggttttca tatccacgat aaggtgaggg
     8641 gaacgttatg tatcaacatc acaactggca aggtgcgctg ctggattatc cggtgagtaa
     8701 agtagtctgt gttggcagta actatgccaa acatattaaa gagatgggca gcgcagtgcc
     8761 cgaagagcca gtgctgttta ttaaaccaga aacggcactg tgcgatctgc ggcagccatt
     8821 ggcgatccca tccgatttcg gttcagttca tcatgaagtc gaactggcgg tgttgattgg
     8881 cgcgacgctg cgtcaggcta cggaagagca tgtccgcaaa gccattgccg gttatggcgt
     8941 ggcgctcgat ctgacattgc gtgatgttca gggaaaaatg aagaaagccg ggcagccgtg
     9001 ggaaaaggct aaagcgtttg ataactcttg tccgctttcc gggtttattc ccgcggcgga
     9061 atttaccggc gacccgcaaa atacaacgct gggcctgagc gtaaacggcg agcaacgtca
     9121 gcaaggtacg actgcggaca tgatccataa aattgttccg ctgatcgctt atatgagcaa
     9181 gttttttacc ctcaaggccg gtgacgttgt gctgacaggc acgcctgatg gcgtcggccc
     9241 gttgcaaagc ggtgatgagc tgacagtcac tttcgatggg cattctttgg caactcgcgt
     9301 tttgtaatac tttttgccgc ctgaaagcgg cggcaaaact tgcatcgctg tgccagactg
     9361 gttataaggt gcgttttaac gtaatggcgg aacacctgat gagcgatgta cctttctggc
     9421 aaagtaaaac cctggacgaa atgagcgatg cggaatggga gtcgttgtgt gatggttgcg
     9481 gtcagtgttg cctgcataaa ctgatggatg aagacaccga cgaaatctac ttcactaacg
     9541 tcgcctgtcg ccagctcaat attaaaacct gtcaatgtcg gaactacgaa cgtcgtttcg
     9601 agtttgaacc cgactgcatt aaattaaccc gtgaaaatct gccaacattc gaatggctgc
     9661 caatgacctg cgcttatcgt ttgctggcgg aaggtaaaga tttacctgcg tggcatccgc
     9721 tacttactgg ttcgaaagcg gcaatgcatg gtgaacgtat ctctgtgcgt catatcgcag
     9781 tgaaagaatc agaagtgatt gactggcagg atcatatctt aaataaacct gactgggcgc
     9841 agtgataaat ttaatagatg ttgatggggg ctttcttaat atacgtctgg atgctcgcca
     9901 tttcgtagcc ctgtttttga aagcgctctg tcataatgag agttacccga taccttatta
     9961 tcaacaggta actctcaatc tttaaaaaac gtctgtgttt ttaaatgtga taaaccttat
    10021 accttgagta catggagaga ataatcgctt atcagacttc aggtacctca aagagtgtct
    10081 ttttaccgtg tcttttctga tactcattac aggtgttaat catttttttg gccgcttctt
    10141 ttagcaaaga aagcattaaa tcatcataat caacatagaa tctggttgtt tcagtttccg
    10201 tttttatctc cccgatggcg gctatttcgg tggttaattt caatttggcg gcatcgatat
    10261 ctttattcgc ttgtttaacc gtgttagaca gggtggtaaa gaaactctgc acagatttta
    10321 acttgttctt caattctggg atcagtttcc cttcaactac gcccgcagca atagaatagg
    10381 aaatgattaa tccaaatgga ccagcgacga caccggctgc ggcaccggca tacgcttcct
    10441 tcctgatttt atctacctgt gactggaaat agctgctttt ttctgaaaaa tcattggtta
    10501 actggctatc taacgccagc agtttcccgg aagcgttgtt gaaactttgt gagcttacca
    10561 gcagggattt ttgcgcttca ttcagcttcg tgatgccgtc atccagtacc ttaatgagaa
    10621 tgtctttctg ggcggatgct ttcttctcat tgtactcatc aaatagcaaa atatacgctg
    10681 cgagcaattg cgtcgcaaca ccacaccatt catacactgt ttgggttgct tcaaaatact
    10741 tatcctggct atccataagt aaggttttaa tatcgccgac taaaacggag gctgcctgtg
    10801 aatactcctg tttaaagcga cttaactctt ttatggtttc atcaaatgtc tgccagggga
    10861 tgacctgatc gagatattta ttataaagat ctaatgctcc atctgcggtt tcgattgcgt
    10921 ttttaactac ctctaccgtt ttatctgcaa c
//
LOCUS       DAPHDW010000146        10950 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.148, whole genome shotgun sequence.
ACCESSION   DAPHDW010000146 DAPHDW010000000
VERSION     DAPHDW010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10950
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.148"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            182..1396
                     /locus_tag="RH057_003743"
     CDS             182..1396
                     /locus_tag="RH057_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HDV3130050.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPAYLP
                     VARLEDGTLWNW"
     gene            1463..2746
                     /locus_tag="RH057_003744"
     CDS             1463..2746
                     /locus_tag="RH057_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3130051.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILMRIGLGIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQ
                     AIAPPALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSAPV
                     KPRPQLQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGW
                     VAAIPFLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSP
                     AQAVAFISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGW
                     VVDTTHSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            2998..3915
                     /gene="oxyR"
                     /locus_tag="RH057_003745"
     CDS             2998..3915
                     /gene="oxyR"
                     /locus_tag="RH057_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HDV3130052.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVPMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPRRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(3898..5298)
                     /gene="sthA"
                     /locus_tag="RH057_003746"
     CDS             complement(3898..5298)
                     /gene="sthA"
                     /locus_tag="RH057_003746"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HDV3130053.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIEGCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            5629..6279
                     /gene="fabR"
                     /locus_tag="RH057_003747"
     CDS             5629..6279
                     /gene="fabR"
                     /locus_tag="RH057_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HDV3130054.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            6279..6638
                     /locus_tag="RH057_003748"
     CDS             6279..6638
                     /locus_tag="RH057_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HDV3130055.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMPVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(6678..7778)
                     /gene="trmA"
                     /locus_tag="RH057_003749"
     CDS             complement(6678..7778)
                     /gene="trmA"
                     /locus_tag="RH057_003749"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HDV3130056.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IELDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQCETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      7892..8082
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            8147..9991
                     /gene="btuB"
                     /locus_tag="RH057_003750"
     CDS             8147..9991
                     /gene="btuB"
                     /locus_tag="RH057_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HDV3130057.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTANRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHFGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVENGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            9936..10793
                     /gene="murI"
                     /locus_tag="RH057_003751"
     CDS             9936..10793
                     /gene="murI"
                     /locus_tag="RH057_003751"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HDV3130058.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PYLHYIYAFDNVAFPYGEKSEEFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2518 a         2596 c         2931 g         2905 t
ORIGIN      
        1 acgtgttgtt ttaacgatgg tggttaatta ttcaggtggt ttttgagggt attttttctc
       61 ctcggcggaa atttttattt ttaatgtgat cacggttgaa taatcgctct ttctctcttg
      121 tatggtagta ggcgtaaaat taagtttgaa gcgtctattt acaacacagg agattgcaat
      181 aatgaaaatt attgctgcag atgtttttgt tacttgccca gggcgtaatt tcgttacgct
      241 taaaataacc actgaaagcg gactttgtgg attaggagat gccacattaa atggtcgcga
      301 attgtcggtc gcttcttatc tgaaagatca tctttgtcca caactaattg gccgcgatgc
      361 cagccgtatc gaagatatct ggcaattctt ttataaaggt gcttactggc gacgtggccc
      421 cgtcacgatg tcagccattt ctgccattga tatggcgttg tgggatatca aagctaaagc
      481 ggcaaatatg ccattgtatc aactgcttgg tggtgcttct cgtgaaggcg tgatggttta
      541 ttgccacacc accgggcgga cgattgatga agtccttgaa gactatgcaa agcaccagca
      601 gatgggcttt aaggcgattc gtgtgcagtg cggtgtaccg ggtatgcaaa ctacttatgg
      661 cctggcgaag ggcaaaggcc tggcgtatga accggcaacc aaaggcctgt ggccagaaga
      721 gcagttgtgg tcttcagaaa agtatctcga ttttacgccg aagttatttg aagcggtgcg
      781 taataagttc ggttttaacg aacatctgtt gcatgacatg caccatcgcc tgacccccat
      841 cgaagcggcg cgctttggga agagcattga ggattaccgc ctgttctgga tggaagatcc
      901 gacgcctgca gagaaccagg aatgttttcg tctgattcgc cagcataccg tgacgccgat
      961 tgcggtcggt gaagtgttta acagtatctg ggactgcaag cagttaatcg aagagcagct
     1021 gattgactat attcgcacga cgatcaccca tgcaggcggc attaccggga tgcggcggat
     1081 tgccgatttt gcatcgcttt atcaggtacg taccggttcg catggccctt cagatttatc
     1141 gcctgtttgt catgcggcgg cgctgcattt tgacctatgg gtaccaaatt ttggcgttca
     1201 ggagtacatg ggctattccg aacaaatgct tgaagttttc ccgcacagct ggcgcttcga
     1261 ggaaggttat atgcacccag gcgatgaacc ggggctgggt atttctttcg atgagaaact
     1321 ggcggcgaaa tatccgtatg acccggcgta tctccccgtc gcacgactgg aagatggcac
     1381 attgtggaac tggtaataac ggttcgctta attattttct gaatagtcat caaatcgatt
     1441 aattccgctc aaggagctaa aaatggacgt tgatgtgtca accagtgttg cgggtaataa
     1501 accgcaacgg attcgtcgta ttcagacagt gaccctggtt ttattattta tggcggggat
     1561 cgttaatttt ctcgaccgtt cgtcattgag cgtggcaggt gaagcgattc gtggcgagtt
     1621 aggattatcg gcgacagagt ttggtgtttt gctttctgca ttttctctgt cttatggttt
     1681 ttcacaacta ccttccggta ttttgctaga ccgctttggc ccacgaattg ttttaggcgc
     1741 aggcttaata ttctggtcat taatgcaggc attaacagga atggttaatt cttttagcca
     1801 ctttatttta atgcgtatcg gtctgggtat tggcgaagcg ccatttatgc ctgcgggagt
     1861 aaagtcgatc accgactggt atgcacaaaa agaacgcggc acggcgctgg ggatttttaa
     1921 ctcatctacc gttatcggtc aggctatcgc gcctcctgct cttgtattga tgcagctggc
     1981 atggggctgg cggacgatgt tcgttatcat cggcgtggca gggattctgg ttgggatctg
     2041 ttggtacgcg tggtatcgca accgggcgca gtttgtcctg actgacgaag aacggacgta
     2101 tctctccgcg ccggttaaac cgcgtccaca gctgcaattt agcgagtggc tggcgctgtt
     2161 taagcatcgg acaacctggg ggatgatttt aggtttctct ggcgtcaatt ataccggttg
     2221 gctctacatc gcgtggttac ccggttattt gcaagccgag caaggtttca gtctggcgaa
     2281 aaccggttgg gtggcggcga ttcctttcct cgcggcggca gtcgggatgt gggttaacgg
     2341 tattgttgtc gatcgactgg cgaaaaaagg ctacgacctg gcgaagacgc gtaaaacggc
     2401 tattgtctgc ggtttgatga tgtcggcatt aggcacgttg ctggtcgtgc aatcttcctc
     2461 gccagcccag gcggtagcgt ttatctcaat ggcgctattc tgcgtgcatt tcgctggaac
     2521 gtctgcatgg gggctggtgc aggtgatggt gtcagaaaca aaagtggctt ccatcgccgg
     2581 gattcaaaac tttggcagtt ttgtttttgc ttcctttgct ccgatcgtaa ccggttgggt
     2641 agtggatacc acacactcgt ttaatctggc gttggttatc gcggcctgtg tgacgttcac
     2701 cggggcgctg tgttacttct ttattgtcaa agatcgcatt gagtaatcgt tactatcagg
     2761 ctctcttgct gtgggcctgt agaataaaaa aaagcggatc ctggagatcc gcaaaagttc
     2821 acgttggctt tagttattcg agttgagaaa ctctcgaaac gggcagtgac ttcaagggtt
     2881 aaaagaggtg ccgctccgtt tctgtgagca attatcagtc agaatgcttg atagggataa
     2941 tcgttcattg ctattctacc tatcgccatg aactatcgtg gcgatggagg atggataatg
     3001 aatattcgtg atcttgagta cctggtggca ttggccgagc accgccattt tcggcgtgcg
     3061 gcagattcct gccacgttag ccagccgacg cttagcgggc aaattcgtaa gctggaagat
     3121 gagttgggcg tgatgttgct ggagcggacc agccgtaaag tgttgttcac ccaggcggga
     3181 atgctgctgg tagatcaggc gcgtaccgta ctgcgtgagg tgaaagtcct taaagagatg
     3241 gcaagccagc agggcgagac gatgtccggg ccgctgcaca ttggtttgat tcccacagtt
     3301 ggaccgtacc tgctaccgca tattatccct atgctgcacc agacctttcc aaagctggaa
     3361 atgtatctgc atgaagcaca gacccaccag ttactggcgc aactggacag cggcaaactc
     3421 gattgcgtga tcctcgcgct ggtgaaagag agcgaagcat tcattgaagt gccgttgttt
     3481 gatgagccaa tgttgctggc tatctatgaa gatcacccgt gggcgaaccg cgaatgcgta
     3541 ccgatggccg atctggcagg ggaaaaactg ctgatgctgg aagatggtca ctgtttgcgc
     3601 gatcaggcaa tgggtttctg ttttgaagcc ggggcggatg aagatacaca cttccgcgcg
     3661 accagcctgg agacgctgcg caacatggtg gcggcaggta gcgggatcac tttactgcca
     3721 gcgctggctg tgccgccgga gcgcaaacgc gatggggttg tttatctgcc gtgcattaag
     3781 ccggaaccac gccgcactat tggcctggtt tatcgtcctg gctcaccgct gcgcagccgc
     3841 tatgagcagc tggcagaggc catccgagca agaatggatg gccatttcga taaagtgtta
     3901 aaacaggcgg tttaaaccgt ttaacgcagc tacccgatag gcttccgcca tggtcgggta
     3961 gttaaaggtg gtgttgacga agtactcaat agtgttgccg ccacctttct gttccataat
     4021 cgcctgaccg atatgaataa tttcggcagc gcgctcgcca aagcagtgaa tacccagaat
     4081 ctcttttgtt tcccgatgga acaaaatttt cagcgtgccc acgttcatgc cgacgatttg
     4141 tgcgcgtgcc agatgtttaa actgggcgcg gcccacttca tatggcactt tcatcgcggt
     4201 cagctgctgt tcggttttgc ccacagagct gatttccggg atggtgtaaa tgccggtagg
     4261 gatatcttca atcagatgcg cggtggcttc gccttttacc agcgcctgcg cggcaatgcg
     4321 cccctggtca taggccgctg atgccaggct cggataacca atcacgtcgc ccaccgcgta
     4381 aacgtgcggc tgtgcggtct gatacatgct gttgaccttc agctgtccgc gactgtcagt
     4441 ttccagccca atgttctgta acgccagcga atcggtatta ccggtacgac cgttggcata
     4501 gagcaggcag tcagctttca gttttttacc cgacttcaga tgcatgatca caccgtcgtc
     4561 acagccttcg atcttctcgt actcttcgtt gtgacgaatc actacgccac tgttccagaa
     4621 gtgataggag agagaatctg acatctcttg atcgagaaat gccagcaggc gatcgcgggt
     4681 gttgatcaga tccactttta catccatacc gcggaagatc gacgcatatt cacagccgat
     4741 cactccagca ccatagataa gtacatggcg cggttcgtgg tgcatgctaa gaattgagtc
     4801 gctgtcgtaa atgcgtggat gggtgaaatc aacatctgtt ggatgatatg gacgagagcc
     4861 gcaggcaata acaaattttt cagcggttag tgtttcaacg ctgccgtccg ggcaatccag
     4921 cgccaacgta tgctcgtcaa caaagcgagc gtttccctgc aatatttcac agtgattacg
     4981 ttcgtaaaat ccctgacgca tgcgcgtttg ttgattaatc acgttatcgg catggttaag
     5041 gatatcggca aaagaagagc ggagcagtcg ggaatggtcg ctgtaaagtg ggttttgatt
     5101 gaattctata atgcggctga cggcgtgacg gagagctttc gacgggatgg tgccccagtg
     5161 ggtgcaaccg ccgccaacat tttgataacg ctcgataact gcgacgcgcg caccttgctt
     5221 aaccaggccc attgcagcgc cttcgccgcc ggggccggaa cctattacta tggcatcgta
     5281 atcgtaggaa tgtggcatgg tagggcttac ctgttcttat acataaaagc aacagaatgg
     5341 taacatttta tcgcgggtaa gccaattgat ccccgtcatt tatctggcta tatcctgagc
     5401 ggcctttgct ttgtctgttt cttacttttg ccctgacgtt ttattggatt tttatcgacg
     5461 atactctccg tttaagcggc aggtttccgc tgtacgtaaa agaaccggcc aaagaattgc
     5521 agtaaatatg ttttattgcg ttaccgttca ttcacaatac tggagcaatc cagtatgttc
     5581 attctctggt atagtgccag cagtactttt ggcaaggatt cagacatcgt gatgggcgta
     5641 agagcgcaac aaaaagaaaa aacccgccgt tcgctggtgg aagccgcatt tagccaatta
     5701 agtgctgaac gcagcttcgc cagcctgagt ttgcgtgaag tggcgcgtga agcgggcatt
     5761 gctcccacct ctttttatcg gcatttccgc gacgtagacg aactgggtct gaccatggtt
     5821 gatgagagcg gtttaatgct acgccaactc atgcgccagg cgcgtcagcg tatcgccaaa
     5881 ggcgggagtg tgatccgcac ctcggtctcc acatttatgg agttcatcgg taataatcct
     5941 aacgccttcc ggttattatt gcgggaacgc tccggcacct ccgctgcgtt tcgtgccgcc
     6001 gttgcgcgtg aaattcagca cttcattgcg gaacttgcgg actatctgga actcgaaaac
     6061 catatgccgc gtgcgtttac tgaagcgcaa gccgaagcaa tggtgacaat tgtcttcagt
     6121 gcgggtgccg aggcgttgga cgtcggcgtc gaacaacgtc ggcaattaga agagcgactg
     6181 gtactgcaac tgcgaatgat ttcgaaaggg gcttattact ggtatcgccg tgaacaagag
     6241 aaaaccgcaa ttattccggg aaatgtgaag gacgagtaat gaaacaagca aatcaagata
     6301 gaggtacgct gctgctggcg ttggttgctg gcttatcgat taatggtact ttcgccgcgc
     6361 tgtttagctc cattgtgcca ttttctgtat tcccgattat ttccctggtg ctgacggttt
     6421 actgcctgca tcaacgttat cttaatcgca ctatgccggt aggcttgccg ggtctggcag
     6481 ctgcctgttt tattctcggc gtactgctgt acagcacggt agttcgtgcg gaatatccgg
     6541 atatcggctc taacttcttc ccggcagtac tctccgtcat tatggtgttc tggattggcg
     6601 cgaagatgcg taaccgtaag caggaagttg ctgagtaatc ggtattatgc cggatgctgg
     6661 cgcatccggc atgggtttta cttcgcggtc agtaatacgc cgcactccat atggtgcgtg
     6721 taggggaact gatcaaacag agccagacgt tcgaccttgt gcgtctggct taatgtttcc
     6781 agattcttac ataacgtttc cgggttacag gagatgtaca aaatacgcgg atacgcctgc
     6841 accattttct cggtttcact gtccagaccg ctgcgcggtg ggtcgacaaa aatggtttcg
     6901 cactgataac tctttaagtc gatcccttgc aggcggttaa actcgcgcac accattcatc
     6961 gcctgagtaa attcttctgc cgccatacga ataatttgta cgttatcaat atggttagct
     7021 gcgatgttgt attgcgcagc agcaaccgac ggcttagcga tttcggtggc taatacccga
     7081 tcaaaattgc gcgccagcgc taatgaaaag ttaccgttgc cgcagtacag ctccagtaaa
     7141 tcgccttttg agcctttggt tacgtccagc gcccattcca gcatctgaat attcatcgcc
     7201 gcgttcggct gggtaaagct gttttctacc tgacggtaga tcatctcttt ccctgcgacc
     7261 ggcagacgtt catcgatgta atcttgatcc agctcgattt tggttttcgt tgcccgacca
     7321 atcagatgca cattcagatt ctgcgcgcgc agtgcatcgc gcagggcttc cgcttcctga
     7381 cgccactcat catccagctt tttatggtac aacagggaaa ccaccgcctg attactcagc
     7441 gtggtgaggt aatcaatctg gaacaacttg tggcgcagaa cgggattatt acgcacaccc
     7501 gcaatcatcg ccgtcatcaa ctggttgatg agttcactgg cagcggggaa gctatccacg
     7561 cggatgcggc ttttggtttg ttgatcgaaa atgatgtgat acaggtcatc gccatcgtgc
     7621 cagatgcgga actccgcacg catccggtaa tgactgaccg gcgagcgaaa cacttccgga
     7681 accaggtcag aaaacggtgc catcatactt tgcaaacgta ccactttttc ggctaactgc
     7741 gcttcatact gttctgttgg aaggtgttcg ggggtcatga tgtatcctga aaaattaaag
     7801 tacgcggcga ttgtagggat tgctcatcag atgtccagat cttgatgaat tcctatttgt
     7861 gagctacgtc tggacagtaa cttgttacaa cctgtagcat ccacttgccg gtcctgtgag
     7921 ttaataggga atccagtgcg aatctggagc tgacgcgcag cggtaaggaa aggtgcgatg
     7981 attgcgttat gcggacactg ccatccggtg ggaagtcatc atctcttagt atcttagata
     8041 cccctccaag cccgaagacc tgccggccaa cgtcgcatct ggttctcatc atcgcgtaat
     8101 attgatgaaa cctgcggcat ccttcttcta ttgtggatgc tttacaatga ttaaaaaagc
     8161 ttcgctgctg acggcgtgtt ccgtcacggc cttttccgct tgggcacagg ataccagccc
     8221 ggatactctc gtcgttactg ctaaccgttt tgaacagccg cgcagcactg tgcttgcacc
     8281 aaccaccgtt gtgacccgtc aggatatcga ccgctggcag tcgacctcgg tcaatgatgt
     8341 gctgcgccgt cttccgggcg tcgatatcac ccaaaacggc ggttcaggtc agctctcatc
     8401 tatttttatt cgcggtacaa atgccagtca tgtgttggtg ttaattgatg gcgtacgcct
     8461 gaatctggcg ggggtgagtg gttctgccga ccttagccag ttccctattg cgcttgtcca
     8521 gcgtgttgaa tatatccgtg ggccgcgctc cgctgtttat ggttccgatg caataggcgg
     8581 ggtggtgaat atcatcacga cgcgcgatga acccggaacg gaaatttcag cagggtgggg
     8641 aagcaatagt tatcagaact atgatgtctc tacacagcaa caactggggg ataagacacg
     8701 ggtaacgctg ttgggcgatt atgcccatac tcatggttat gatgttgttg cctatggtaa
     8761 taccggaacg caagcgcaga cagataacga tggtttttta agtaaaacgc tttatggcgc
     8821 gctggagcat aactttactg atgcctggag cggctttgtg cgcggctatg gctatgataa
     8881 ccgtaccaat tatgacgcgt attattctcc cggttcaccg ttgctcgata cccgtaaact
     8941 ctatagccaa agttgggacg ccgggctgcg ctataacggc gaactgatta aatcacaact
     9001 cattaccagc tatagccata gcaaagatta caactacgat ccccattttg gtcgttatga
     9061 ttcgtcggcg acgctcgatg agatgaagca atacaccgtc cagtgggcaa acaatgtcat
     9121 cgttggtcac ggtagtattg gtgcgggtgt cgactggcag aaacagacta cgacgccggg
     9181 tacaggttat gttgagaatg gatatgatca acgtaatacc ggcatctatc tgaccgggct
     9241 gcaacaagtc ggcgatttta cctttgaagg cgcagcacgc agtgacgata actcacagtt
     9301 tggtcgtcat ggaacctggc aaaccagcgc cggttgggaa ttcatcgaag gttatcgctt
     9361 cattgcttcc tacgggacat cttataaggc accaaatctg gggcaactgt atggcttcta
     9421 cggaaatccg aatctggacc cggagaaaag caaacagtgg gaaggcgcgt ttgaaggctt
     9481 aaccgctggg gtgaactggc gtatttccgg atatcgtaac gatgtcagtg acttgatcga
     9541 ttatgatgat cacaccctga aatattacaa cgaagggaaa gcgcggatta agggcgtcga
     9601 ggcgaccgcc aattttgata ccggaccact gacgcatact gtgagttatg attatgtcga
     9661 tgcgcgcaat gcgattaccg acacgccgtt gttacgccgt gctaaacagc aggtgaaata
     9721 ccagctcgac tggcagttgt atgacttcga ctggggtatt acttatcagt atttaggcac
     9781 tcgctatgat aaggattact catcttatcc ttatcaaacc gttaaaatgg gcggtgtgag
     9841 cttgtgggat cttgcggttg cgtatccggt cacctctcac ctgacagttc gtggtaaaat
     9901 agccaacctg ttcgacaaag attatgagac agtctatggc taccaaactg caggacggga
     9961 atacaccttg tctggcagct acaccttctg aaccacgtcc caccgtgctg gtgtttgact
    10021 ccggcgtcgg tgggttgtcg gtctatgacg agatccggca tctcttaccg tatctccatt
    10081 acatttatgc tttcgataac gtcgcttttc cgtatggcga aaaaagcgaa gagtttattg
    10141 ttgagcgagt ggtggcaatt gtcaccgcag tgcaagaacg ttatcccctt gcgctggctg
    10201 tggtcgcttg caacaccgcc agtaccgttt cacttcctgc attacgcgaa aagttcgact
    10261 tcccggttgt tggtgtcgtg ccggcgatta aacctgctgc acgtctgacg gcaaatggca
    10321 ttgtcggatt actggcaacc cgcggaactg ttaaacgttc ttatactcat gagctgatcg
    10381 cgcgtttcgc taatgaatgc cagatagaaa tgctgggctc ggcagagatg gttgagttgg
    10441 ctgaagcgaa gctgcatggc gaagatgttt ctctggatgc actaaaacgt atcctacgcc
    10501 cgtggttaag aatgaaagag ccgccagata ccgttgtatt gggttgcacc catttccctc
    10561 tactacaaga agaactctta caggtgctgc cagagggaac ccggctggtg gattctggcg
    10621 cagcgattgc tcgcagaacg gcctggctgt tagaacatga agctccggat gcaaaatctg
    10681 ccgatgcgaa tattgccttt tgtatggcaa tgacgccaga agctgaacaa ttattgcccg
    10741 ttttacagcg ttacggcttc gaaacgctcg aaaaactggc agttttaggc tgatttggtt
    10801 gaatgttgcg cggtcagaaa attattttaa atttcctctt gtcaggccgg aataactccc
    10861 tataatgcgc caccactgac acggaacaac ggcaaacacg ccgccgggtc agcggggttc
    10921 tcctgagaat tccgacagag aaagcaaaaa
//
LOCUS       DAPHDW010000147        10917 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.149, whole genome shotgun sequence.
ACCESSION   DAPHDW010000147 DAPHDW010000000
VERSION     DAPHDW010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10917
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.149"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            51..812
                     /gene="adiY"
                     /locus_tag="RH057_003752"
     CDS             51..812
                     /gene="adiY"
                     /locus_tag="RH057_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HDV3130059.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            949..2286
                     /gene="adiC"
                     /locus_tag="RH057_003753"
     CDS             949..2286
                     /gene="adiC"
                     /locus_tag="RH057_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HDV3130060.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            2390..4033
                     /gene="eptA"
                     /locus_tag="RH057_003754"
     CDS             2390..4033
                     /gene="eptA"
                     /locus_tag="RH057_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HDV3130061.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILVGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAALIACWIKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPGQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCSKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSE"
     gene            4030..4698
                     /gene="basR"
                     /locus_tag="RH057_003755"
     CDS             4030..4698
                     /gene="basR"
                     /locus_tag="RH057_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HDV3130062.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEAGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            4699..5799
                     /gene="pmrB"
                     /locus_tag="RH057_003756"
     CDS             4699..5799
                     /gene="pmrB"
                     /locus_tag="RH057_003756"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HDV3130063.1"
                     /translation="MNLMHFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(5775..5864)
                     /gene="pmrR"
                     /locus_tag="RH057_003757"
     CDS             complement(5775..5864)
                     /gene="pmrR"
                     /locus_tag="RH057_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HDV3130064.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(5976..7478)
                     /gene="proP"
                     /locus_tag="RH057_003758"
     CDS             complement(5976..7478)
                     /gene="proP"
                     /locus_tag="RH057_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HDV3130065.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKHWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFTGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(7742..8620)
                     /locus_tag="RH057_003759"
     CDS             complement(7742..8620)
                     /locus_tag="RH057_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HDV3130066.1"
                     /translation="MTKTLLDGPGRVLESVYPRFLVDLAQGDDARLPQAHQQQFRERL
                     MQELLARVQLQTWTNGGMLNAPLSLRLTLVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGEQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVALLLGDLRVKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLAGAMVEEIFSVNLA"
     gene            complement(8617..10845)
                     /gene="crfC"
                     /locus_tag="RH057_003760"
     CDS             complement(8617..10845)
                     /gene="crfC"
                     /locus_tag="RH057_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HDV3130067.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELRKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARHELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEVKHEIELALTSANHFLRQQQDALNAQLAALFQD
                     DSEPLSEIRTCCETLLQTAQNTISRDFTLRFAELESTLCRELTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHGWIYTDAQLLRDDIQTLFTAE
                     RY"
BASE COUNT         2731 a         2849 c         2691 g         2646 t
ORIGIN      
        1 gctttgcttt agcgagaact ggtcttttat ttgcactcag gagtacatgt atgaggattt
       61 gcagcgacca accttgtatt gttttattga ctgaaaaaga tgtctggata agggtgaatg
      121 ggaaagaacc tattagcctt aaagctaacc atatggcgtt attaaattgt gaaaataata
      181 ttatcgacgt ctcctctctt aacaacactt tggttgctca tattagtcac gacatcatca
      241 aagattacct ccggtttctg aataaagatc tctcgcaaat accagtatgg caacgtagcg
      301 ctacgcccat actcaccctg ccatgcctga cgccagacgt ctttcgcgtt gccgcgcaac
      361 acagcatgat gcccgcagaa actgagtcag aaaaggaacg aacacgcgca ttattattca
      421 cggtgctatc ccgttttctc gacagtaaaa aattcctttc attaatgatg tatatgttac
      481 gtaattgtgt aagtgacagc gtttatcaaa ttattgaaag cgatattcac aaagactgga
      541 atcttagtat ggtagccagt tgtttatgtc ttagcccaag tctgttaaag aaaaagttga
      601 aaagcgaaaa caccagttat agccaaataa tcaccacctg ccgcatgcgt tatgccgtaa
      661 atgaattaat gatggacggt aaaaatatct ctcaggtatc acagtcctgc ggctacaaca
      721 gtacgtcgta ctttatttct gtctttaaag acttctacgg tatgacgccg ctgcattatg
      781 ttagtcagca cagagaacgc actgtcgcct gatttttaac cttaacgaag agctatatta
      841 ataacggcat cagcgataac ccggtcgata ataattcaac tatcgaatgc aggcgtatga
      901 tatgacgtaa tttattgtca cgaagctcgc cttcgcagga gtttaattat gtcttcggat
      961 gctgatgctc acaaagtggg cttaatcccc gtcaccctga tggtgtcggg gaatattatg
     1021 gggtcaggtg tttttctgtt acctgcaaac ctggcctcta ctggcgggat tgccatttat
     1081 ggatggttgg tgacgattat cggtgcactg gggctctcga tggtatacgc caaaatgtcg
     1141 ttcctcgacc caagtcctgg tggttcttac gcttacgccc gccgctgctt tggcccgttt
     1201 ctcggttatc aaaccaacgt cctctactgg ctggcctgct ggatcggcaa tatcgccatg
     1261 gtggtcattg gcgtaggata tttaagttac ttcttcccga ttctgaaaga tccattggta
     1321 ttaaccatca cctgcgtcgt ggtgctgtgg atcttcgtcc tgctgaacat tgtcggtccg
     1381 aaaatgatca cccgtgtgca ggcagttgcc accgtgctgg cgctgatccc catcgtcggg
     1441 attgccgtat ttggctggtt ctggttccgt ggtgaaacct atatggcggc atggaacgtc
     1501 agcggcctgg gcaccttcgg tgcaattcaa agtaccctta acgttacgct gtggtcgttc
     1561 atcggtgtgg aaagtgcctc cgttgccgca ggtgtggtga aaaacccgaa acgcaatgtc
     1621 cctatcgcca ccattggtgg ggtattgatt gccgccgttt gctatgtact ttctaccacc
     1681 gcgattatgg ggatgatccc taatgccgca ctgcgcgttt ctgcttcgcc attcggtgat
     1741 gccgcacgga tggcgttggg tgacaccgcc ggggccattg tttccttctg cgcagctgcg
     1801 ggttgcttag gttcactggg cggctggacg ttgctggcgg gtcaaacggc gaaagccgct
     1861 gccgatgacg gactgttccc accaattttt gcccgtgtaa ataaagcggg tacgccagtg
     1921 gcggggctga ttatcgtcgg tattttgatg accatcttcc agctcagcag catttcacca
     1981 aacgcgacca aagagttcgg tctggtttct tccgtctcgg tcatctttac actggtgcca
     2041 tatctttaca cctgtgcggc gttactgctg ctcggacacg gtcactttgg taaagcacgc
     2101 ccggcatatc tggcagttac taccattgcc ttcctctact gcatctgggc cgtggtgggg
     2161 tccggagcga aagaggttat gtggtcattt gtcaccctga tggtcatcac cgccatgtat
     2221 gcgctgaatt acaaccggct acataaaaac ccgtatccct tagatgcacc aataagcaaa
     2281 gattaattcc ccttaatcca gcaaacataa aagccaacct taagaactta aggttggctt
     2341 aattttgctt tgcgagcata tgcgcacttt gttcgatgga aacaccgtga tgttgaagcg
     2401 cctactaaaa agaccctctt tgaatttact cgcctggcta ttgttggccg ctttttatat
     2461 ctctatctgc ctgaatattg ccttttttaa acaggtgttg caggcgctgc cgctggattc
     2521 gctgcataac gtactggttt tcttgtcgat gccggtcgtc gctttcagcg tgattaatat
     2581 tgtcctgaca ctaagctctt tcttatggct taatcgacca ctggcctgcc tgtttattct
     2641 ggttggcgcg gctgcacaat atttcataat gacttacggc atcgtcatcg accgctcgat
     2701 gattgccaat attattgata ccactccggc agaaagttat gcgctgatga caccgcaaat
     2761 gttattaacg ctgggattca gcggcgtgct tgctgcgctg attgcctgct ggataaaaat
     2821 caaacctgcc acctcgcgtc tgcgcagtgt tcttttccgt ggagccaata ttctggtttc
     2881 tgtactactg attttgctgg tcgccgcact gttttataaa gactacgcct cgttgttccg
     2941 caataacaaa gagctggtga aatccttaag cccctctaac agcattgttg ccagctggtc
     3001 atggtactcc catcagcgac tagcaaatct accgctggtg cgaattggtg aagacgccca
     3061 ccgcaacccg ttaatgcaga acgaaaaacg taaaaatttg accatcctga ttgtcggcga
     3121 aacctcgcgg gcggagaact tctccctcaa cggctacccg cgtgaaacta acccgcggct
     3181 ggcgaaagat aacgtggtct atttccctaa taccgcatct tgcggcacgg caacggcagt
     3241 ttcagtaccg tgcatgttct cggatatgcc gcgtgagcat tacaaagaag agctggcaca
     3301 gcaccaggaa ggcgtgctgg atatcattca gcgagcgggc atcaacgtgc tgtggaatga
     3361 caacgatggc ggctgtaaag gtgcctgcga ccgcgtgcct caccagaacg tcaccgcgct
     3421 gaatctgcct ggtcagtgca tcaacggcga atgctatgac gaagtgctgt tccacgggct
     3481 ggaagagtac atcaataacc tgcagggtga tggcgtgatt gtcttacaca ccatcggcag
     3541 ccacggtccg acctattaca accgctatcc gccgcagttc aggaaattta ccccaacctg
     3601 cgacaccaat gagatccaga cctgtagcaa agagcaactg gtgaacactt acgacaacac
     3661 gctggtttac gtcgactata ttgttgataa agcgattaat ctgctgaaag aacatcagga
     3721 taaatttacc accagcctgg tttatctttc tgaccacggt gaatcgttag gtgaaaatgg
     3781 catctatctg cacggtctgc cttatgccat cgccccggat agccaaaaac aggtgccaat
     3841 gctgctgtgg ctgtcggagg attatcaaaa acggtatcag gttgaccaga actgcctgca
     3901 aaaacaggcg caaacgcaac actattcaca agacaattta ttctccacgc tattgggatt
     3961 aactggcgtt gagacgaagt attaccaggc tgcggatgat attctgcaaa cttgcaggag
     4021 agtgagtgaa tgaaaattct gattgttgaa gacgatacgc tgttattgca gggattgatt
     4081 ctggcggcgc aaaccgaagg ctacgcgtgc gatggcgtga caaccgcgcg gatggcggaa
     4141 caaagccttg aggccggtca ttacagcctg gtggtactgg atttagggtt acccgacgaa
     4201 gatggactgc attttctcgc ccgtatccgg cagaaaaaat acaccctgcc agtactgatc
     4261 ctcaccgctc gcgatacgct gaccgacaaa atcgccgggc tggatgtcgg tgccgatgac
     4321 tatctggtga agccttttgc gctggaagag ttacacgccc gtatccgcgc cctgctacga
     4381 cgccataata atcagggcga aagtgagctg attgttggca atctgacgct gaatatgggt
     4441 cgccgtcagg tatggatggg cggtgaagag ttgattctga cgcccaaaga atatgctctg
     4501 ctgtcacggt taatgctcaa agcaggcagt ccggtgcatc gggaaattct ctacaacgac
     4561 atctataact gggacaatga accctcgacc aacaccctgg aagtgcatat ccacaatctg
     4621 cgcgacaaag tgggcaaagc ccgtatccgc accgtgcgcg gctttggcta tatgctggtc
     4681 gcgaatgagg aaaactaatt gaatctgatg cattttctgc gccgaccaat atcgctgcgc
     4741 caacggctga tattgaccat cggggccatt ttgttggtgt ttgagctgat cagcgtcttc
     4801 tggctatggc atgaaagtac cgagcagatt cagctgtttg agcaggcgct acgcgacaat
     4861 cgcaacaacg atcgccacat catgcgtgag atccgcgagg cggtcgccag cctgattgtc
     4921 cccggcgtgt ttatggtcag cctgacgcta tttatctgct atcaggcggt acgccgcatc
     4981 acccgcccgc tggcggagct gcaaaaagag ctggaagcgc gcaccgccga caacttaacg
     5041 cccattgcca ttcacagcgc caccctcgaa atcgaagcgg tggtttcggc gttgaacgat
     5101 ctggtcagtc gcctgaccag cacgctggat aacgaaaggt tgtttaccgc tgacgtcgcg
     5161 cacgaactgc gaacgccact ggctggggtg cgtttgcatc tggaactgct ggcgaaaacg
     5221 catcacattg atgtagcacc gttagtggca cggcttgatc agatgatgga gagcgtctcc
     5281 cagctgctgc aactggcgcg tgccggacag tcattttctt ccggtaatta tcaacatgta
     5341 aaactgctgg aagatgtcat tctcccctcg tatgacgaac tcagtaccat gctcgaccag
     5401 cgacagcaaa ccctgctatt gccagagagc gccgccgaca tcaccgttca gggtgatgca
     5461 accctgctgc ggatgttatt gcgaaacctg gtagaaaacg cccatcgtta cagcccgcaa
     5521 ggcagcaaca ttatgattaa gctgcaagaa gatggcggag cggtcatggc agttgaagat
     5581 gaaggaccag gtattgatga gagtaaatgc ggggagttga gtaaagcgtt tgtacgtatg
     5641 gacagccgtt atggcgggat tggtctgggg ttaagtattg tcagccgcat tacacagttg
     5701 catcacgggc agtttttcct gcaaaaccgg caagagactt ccggcacgcg ggcctgggta
     5761 cggctgaaga aagatcagaa cgtggcaaac cagatataaa gaaagctgct gaccaccagc
     5821 acgctgaaca cggtagttaa actttcataa acacggtttt tcattgcact ctcctccgtt
     5881 aacctggagg agagtatgcg cgtcagagat taagtgaacc ttaagagttc aattggcctg
     5941 ataagacagc gtcacatcag gccatccgtt tcagcttatt catcaattcg cggatgttgc
     6001 tgcaccaggc gggtacgttt cgcctgcaaa tcggcaatct cgtggtcaat attatcgatt
     6061 ttctgctcga tattatcgta atgctcgacg agaatttcct tcgcttcctg tatatctgac
     6121 gccgccggtg tcgcaccttt caacggacga tttgccgtct ctttcatggt tacgccggtg
     6181 attaaaccaa tcaccgccac taccatcagg taataggcag gcatcatcag attctgcgag
     6241 ctttcgacca gccaggccgc cagtgttggc gtcagaccgg caaccagcac cgaaatatta
     6301 aatgccgccg ccagcgcgct gtaacggata tgcgtcggga acatcgctgg caaggtagaa
     6361 gccataacgc ccgtaaagca gttaaggatc accgccagca tcagtaaccc ggcaaaaatc
     6421 aggccgatga cgttactgtt aatcagaata aacgccggga tcgccaacac aaacagggca
     6481 acactaccaa gtagcacaaa cggacgacgg ccaaaacggt cactcagcaa gcccatcacc
     6541 ggctggacaa acagcatacc gatcataatg gcgataataa tcagcacccc gtggtcttcg
     6601 gagtaatgca ggttatgcga caaataactc ggcatatagg tcagcaacat gtagtaagtc
     6661 acgttggtgg caattaccag accaatacat gtcaacaggc tgcgccagtg tttggtggca
     6721 atctctttaa acgagacttt cgggccatcc tgcaaacctt cgcggtcgcc ctgttccagt
     6781 ttatcgacat gctgctggaa cgccggagtt tcttccagcg catggcgcag gtaaagcccg
     6841 ataatcccta acggcagagc aataaagaac ggaatacgcc agccccagtc gaggaagttc
     6901 gcttcgccga caatggtcga aattaacacc actacgccag cacccagcac aaacccggca
     6961 atagaaccaa agtccagcca gctgcccata aagccacgtt tacggtcagg ggagtattcc
     7021 gcaacaaata tcgacgcccc ggtatattca ccgccgaccg agaaaccttg tgccatctta
     7081 cagatcaaca gcagaatcgg tgcccaaata ccaatcgtgt cgtaggacgg tattaagcca
     7141 atacagaacg tactgatcga cataatcaca atagtgatag cgaggatctt ctggcgacca
     7201 tatttatcgc ccaacatacc aaagaagagt ccgccaagcg gtcgaatcag aaagggaacg
     7261 gagaacgtgg caagtgcagc aaccatctgc acgctggggt cagcccccgg gaaaaaaact
     7321 ttacctaatg cgtaagcaac aaaaccataa acaccaaaat caaaccattc cattgcatta
     7381 cccagtgatg ctgcggtaat ggctttacgc agtttaccgt catcaataat ggtgacatca
     7441 cgaagggtaa tcggttttac ttttttcctt ttcagcatag ctttcctcgc agagttggcc
     7501 catttccgca aacgccagaa aaatcagcgc gacggccgca aaaggggagc gggaattgca
     7561 ggatgcaatc tctgtaacaa gcgtagcggg tttgcttaca ccctccggtt aaggaatgta
     7621 caatcaaacc agttgacgcc tgaattgggc agttaatgac ctttttaccc taccagtgtt
     7681 taaatttgtg atcaacttca cacatggatt tcgtttagga ctcattgatg taactgatta
     7741 tttacgccaa atttacagag aagatttcct cgaccattgc ccccgccaga cgctgcgctg
     7801 aacagagtcg tggcatcccc agcgcgacag tttgccagcg ttgggtcact gaccagcaaa
     7861 cgggttgttt atctgccgtg gtccactttc ccagccaact taaaaaatca gcatgatccc
     7921 cgcccgtcgg aacggtcgaa gaggcaaacc actgatggta atagtgacgc gtggtcgggg
     7981 ccgcattgat actctctgcc agatgttgtg tcgctttcac gcgcaaatca cccagcaaca
     8041 acgccaccgc ttgcgggctt cccagacgac aggcatcgcc aaatgccccc caacgcaact
     8101 cttcgagaac gataaaacag cgcccaggca acgaccaggc gttatacgcc ccagcacgcc
     8161 agcgggtaaa aatttgctcg ctctgttcac ccgcctgcac cagtaggcct cgttgcgtta
     8221 atgccttttc cttccagcga caacgggctg aaaaccactc atacaaggcg gcaatttgct
     8281 gggggagctc cgggaaagca gagtgctggc ggtgatccat tttttgcagc agcgccagat
     8341 gctgcgcgat ctgcgtcagt gccagatgac cgggatccag catcgacgcc agtttttcca
     8401 ccaatgtcag acgcaggcta agcggcgcat ttaacatgcc gccgttcgtc catgtctgaa
     8461 gctgcacgcg cgcaagaagt tcctgcatca gtcgttcacg aaactgctgc tgatgggctt
     8521 gtggaaggcg ggcatcatcg ccctgcgcca gatccactaa aaagcgggga taaaccgact
     8581 ccagcacgcg accggggccg tcaagtaacg tcttggtcaa tatcgttctg ccgtgaaaag
     8641 tgtttgaata tcatcgcgta acagctgggc gtcggtgtaa atccagccgt gagtcatcac
     8701 agtctgctgc aattgctgct gcatcagcct gaccaccgat tcattttgtt gacgcagagc
     8761 cagactttcg cgtaaacgcg tctgtaattc cgtcaaacat gaagcgaact cagcgaaaaa
     8821 agtattcatg cctgccgtaa cagattcatc gacctgctct gccagaactt tagccatttg
     8881 ttggcaataa agatcgactt ctgcgcttaa agctcgttgc aacacactgt aatcaaccgt
     8941 ttctgtcggg gatttctcat ttccccgtcc ccagtcgggc tgattcaacc agcgcgaaaa
     9001 agtctcacgc acaacgccta aacgcgtgct ctgctcgtcc gttgcttcct ggcgcgaaat
     9061 gacttcactg aacagttggc gagtgttgaa gtggggaact acgccgtgaa aaacaggaaa
     9121 atgaaaccca ggacgaaacc ctgactcgct caattccatt ttgacttgtt gctcaatggg
     9181 ccgaataaca tcggttaact cccggcaaag ggtggattca agctcggcaa aacgcagcgt
     9241 aaagtcgcga ctgatggtgt tctgcgccgt ctgtaacagt gtctcacagc aggtacgaat
     9301 ctcgcttaac ggctccgaat catcctgaaa caaggcggct aactgcgcat tcagcgcatc
     9361 ttgctgttga cgcagaaagt ggttggcgga ggtcagggcc agctcgattt catgtttaac
     9421 ctcgccgctc acctgcgcct gattgagttg caatagctgc aaactttctt cgacctgatg
     9481 gatattttgc cgcaattgtt cacaagcgac gtttaacccg tgcgcacgaa aatccaggta
     9541 ttcccgcgcc tgctgcgcgt aattcaacag tttatgcgca gcagatcgca aagcatacaa
     9601 cgaggcgtta gcgtaagcgg catgaagcaa cgcctgaatt ggctgggcga acagcgaatc
     9661 ttcccacaac tgatcggcag catgacgaat atgttcgagg tccgccagat cggcatgacg
     9721 ccagcgcctg ccgagcgcgg catgggcaaa atcttccacc cagcgttgtt gctctggcgc
     9781 tggtaactta ccgttgttgg ctaactcatg gcgcgcccga ttcgccaggt agccccacat
     9841 cgacgacacc ggaaatatct gctgtggcgt aatacagcct ttcatcagcg tcccggaaat
     9901 cagtgcccgc acctggtcgg cgtcgtcact gttacgatcc tgttgatcga acttattgac
     9961 cagcacatac agcggcaccg attgccccac cgccaaaatc gcctcacgga cctcttcatc
    10021 ggagatcgat ttcagttgcg tataatccag caccgccagt accgccgagg cgcgtgccag
    10081 ctgctggtta agcatttttt gcagatgcgg ttgcccggct tcatttggcc cgggggtatc
    10141 cagtaacgtc aactgaccgg gataactctc cagccccgcc agatggacaa actccacttc
    10201 aatcacggga atatgctcaa tggcggcgta agcagaaaaa ggaaaatcga cgtccagcgc
    10261 cttcgccagt cgcactaaat cattcaaact tttcagacaa tgaaaaatag gctgggcacc
    10321 cagataatat ttttcgaaag cgacgccatt ttcgatccgc tgcataagcg cacgcatatc
    10381 tttatctatt tccagcacat cggtcagatg cttaatatcg caatcacgca ggcgctgttg
    10441 taattgttga attaaacaat cgattggcgc gacatgtgaa aaatgcagta ccggttcctt
    10501 ttgcccgggc gtatggcgaa taagcgtcgg cagcgcagtc attgggcgat tacgattagg
    10561 cagaacctcc gtaccaacaa tggcattaat ggtggttgat ttccctgctt tcatggtacc
    10621 gacaattgcc agcaccattt ccagtcggga aattttacgc aactcattat tcagcatcgc
    10681 gtgacgttcg gcgatattag gctgactcca gggtaaagcc agttgtggcg cgtcgtctcc
    10741 gggtacagag agaggcattt tttccagtaa ctgcaactgt tggcgagaaa gctgtaacag
    10801 gcgttcagcc tcctgactta actcatacag ggtctgtgtg tacatagaaa attcttcctt
    10861 aaagcaaatt ttgttacttt tatttagcca gattgttttt gagttctgtt ttcggct
//
LOCUS       DAPHDW010000148        10867 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.150, whole genome shotgun sequence.
ACCESSION   DAPHDW010000148 DAPHDW010000000
VERSION     DAPHDW010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.150"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(102..1766)
                     /gene="glnS"
                     /locus_tag="RH057_003761"
     CDS             complement(102..1766)
                     /gene="glnS"
                     /locus_tag="RH057_003761"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HDV3130068.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHTTVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVESIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(1969..3915)
                     /gene="nagE"
                     /locus_tag="RH057_003762"
     CDS             complement(1969..3915)
                     /gene="nagE"
                     /locus_tag="RH057_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HDV3130069.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVVFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLNQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLIIKAQGHVVAGQTPLYEIKK"
     gene            4248..5048
                     /gene="nagB"
                     /locus_tag="RH057_003763"
     CDS             4248..5048
                     /gene="nagB"
                     /locus_tag="RH057_003763"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HDV3130070.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMILVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            5108..6256
                     /gene="nagA"
                     /locus_tag="RH057_003764"
     CDS             5108..6256
                     /gene="nagA"
                     /locus_tag="RH057_003764"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HDV3130071.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIGVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            6265..7485
                     /gene="nagC"
                     /locus_tag="RH057_003765"
                     /gene_synonym="nagR"
     CDS             6265..7485
                     /gene="nagC"
                     /locus_tag="RH057_003765"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HDV3130072.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            7533..8285
                     /gene="nagD"
                     /locus_tag="RH057_003766"
     CDS             7533..8285
                     /gene="nagD"
                     /locus_tag="RH057_003766"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
                     /protein_id="HDV3130073.1"
                     /translation="MTIKNVICDIDGVLMHDNVAVPGAAEFLHGIMDKGLPLVLLTNY
                     PSQTGQDLANRFATAGVDVPDSVFYTSAMATADFLRRQEGKKAYVVGEGALIHELYKA
                     GFTITDVNPDFVIVGETRSYNWDMMHKAAYFVANGARFIATNPDTHGRGFYPACGALC
                     AGIEKISGRKPFYVGKPSPWIIRAALNKMQAHSEETVIVGDNLRTDILAGFQAGLETI
                     LVLSGVSSLDDIDSMPFRPSWIYPSVAEIDVI"
     gene            8542..10206
                     /gene="asnB"
                     /locus_tag="RH057_003767"
     CDS             8542..10206
                     /gene="asnB"
                     /locus_tag="RH057_003767"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HDV3130074.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKTHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNAKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            10587..10663
                     /locus_tag="RH057_003768"
     tRNA            10587..10663
                     /locus_tag="RH057_003768"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10621..10623,aa:Met,seq:cat)
     gene            10672..10756
                     /locus_tag="RH057_003769"
     tRNA            10672..10756
                     /locus_tag="RH057_003769"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10706..10708,aa:Leu,seq:tag)
     gene            10780..10854
                     /locus_tag="RH057_003770"
     tRNA            10780..10854
                     /locus_tag="RH057_003770"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10812..10814,aa:Gln,seq:ttg)
BASE COUNT         2691 a         2811 c         2769 g         2596 t
ORIGIN      
        1 gaaaagcaga aaaaacgccg cagagtgcgg cgtttgggat tgtttgctat ttcttgccgg
       61 atgcgacgta aatgcctcac ccggcctggt agaacttaaa attactcgcc tactttcgcc
      121 caggtatcac gcagcccaac ggtgcggtta aataccggtt tttccgctgt ggaatggcgg
      181 ctgtcgaggc agaagtaacc ttcacgctca aactggaatg ctttacccgc aaccgcatct
      241 ttcagcgacg gttcagcaaa gccctgtttg atcaccagcg attccgggtt aatcaccgac
      301 aggaaatcat ccgcagcacc agggttcggc acgctgaaca gacggtcgta cagacggatt
      361 tcaaccggca gcgcatgtgc cgcgctcacc cagtgaataa cacctttgac tttacgacca
      421 tctgccggat ctttgcttaa ggtgtcggcg tcataagtac agaagatggt ggtgatattg
      481 ccttcggcat ctttctccac gcgttcagcc ttaatgacgt aagcattacg cagacgcact
      541 tctttaccca gcaccagacg tttgtactgc ttgttagctt cttcgcggaa atcggcgcga
      601 tcgatccaaa tctcaccgct aaacggcacc tgacgactcc ccatttccgg tttgttcgga
      661 tggttcggca tggtgaccat ttcgccttcg ccctgatagt tttcgataac cagtttcacc
      721 ggatcgataa ccgccattgc gcgcggcgca ttttcgttga gatcttcacg gatgcaggat
      781 tccagcgatg ccatctcaat ggtgttgtcc tgcttggtca cgccgatgcg tttgcagaac
      841 tcacgaatag aagcagcagt gtaaccacga cgacgcagac cggaaatggt cggcatacgc
      901 gggtcatccc agccttcaac gtgcttgtcg gtcaccagca ggttcagctt acgcttggac
      961 atcacagtgt attccagatt caggcgcgag aattcgtact ggcgcgggtg aacaggaatg
     1021 gtgatgttgt ccagcaccca gtcgtacagg cgacggttgt cctggaactc aagcgtacac
     1081 agagagtgcg taataccttc cagggcatcg ctgatgcagt gggtgaagtc gtacatcggg
     1141 tagatgcacc acttgttgcc agtctggtgg tgttcagcaa atttaatacg gtacagcacc
     1201 ggatcgcgca tcacgataaa cggtgaagcc atgtcgattt tcgcacgcag gcaggcttta
     1261 ccttcttcaa aaccaccggc acgcattttt tcgaacagcg ccaggttctc ttcaacgctg
     1321 cggtcgcggt acgggctgtt tttacccggt tgcgtcaggg tgccgcggta ttcgcggatc
     1381 tgttccggcg tcagttcatc aacgtacgcc aggcctttat tgatcagttc gatcgcatag
     1441 gcgtggagct gatcaaaata atcggaggag taacggacgt taccagacca gtgaaaacct
     1501 aaccactcta cgtcgttttt gatcgactca acatactcga tatcttcttt taccgggtta
     1561 gtgtcgtcga aacgcaggtt gcactggcct ttatagtcct gggcgatccc gaagttcagg
     1621 cagatagatt tcgcatggcc aatatgcaga tagccattcg gctccggcgg gaaacgggtg
     1681 tgtactgtgg tgtgcttacc actggccaga tcttcatcga tgatctgacg gataaagtta
     1741 gtcgggcggg cttctgcctc actcatcgtg gattcctcaa agcgtaaaca acgtataacg
     1801 gcgtatgatc ttataagcgg gacaggctga caactgttag ttttttaaaa ttgataatct
     1861 aacaaggatt atggggatga ttgatgaaaa aaaaagcggc agagaagatc tccaccgctt
     1921 gtcattgttg gatgcgacgc tcaagcgtcg catcaggcat aaagcagatt actttttgat
     1981 ttcatacagc ggtgtttgac ccgccacaac atggccctga gctttaatga tcaagccact
     2041 gaaatcgtcg atattgctgc aaaccaccgg gctaatcatc gagcgggcgt tagcgttcag
     2101 gtaatccaga tccatttcca gaatcggttg ccctgcgctt acctgcgcac cctcttccac
     2161 cagacgttta aagcctttac cttccagcgc tacggtgtcg atacccatat ggacgacgat
     2221 ctccgcgcct ttttcggttt ccaggcagaa cgcgtggttg gtgttgaaga ttttcacgat
     2281 tgtccctgcg gctggtgata cgacgatttt atctgtcggt ttcaccgcca caccgtcacc
     2341 caccgctttg ctggcgaatg cttcgtcagg aacctgatcc agtgccacga catcaccggt
     2401 aatcggcgat accagctccg cgatagatac cgcgtttggt acagcctgcg gttttgctac
     2461 aggcgcggca gttgccggag ttgcttcagc tgacgcagct gctaccggac cgcgggcaac
     2521 gactttcttc atcgcatcgc cgatggattc tgctttcgcg ccaacaatca cctgaatagt
     2581 ctgtttgttc agttttacta caccagaagc acccagacgt ttacacatcg tatcgttaac
     2641 gcgggcagag tcagccactg taaggcgcag acgggtgata caggcgtcaa ttgctttcag
     2701 gttgtcagtg ccgccaaccg cagcaatata gttggtcgcc agttgattga ggccttcttc
     2761 agtgttgctg ttggcttctt cagtaacgat ctcgtcttct ttatcttcac gacccggcgt
     2821 tttcaggttg aacatgcgga taaccaaact gaacaccacg aagtagatag cgaagaagac
     2881 aacacccatc accagcagca tccagacgtt ctggctggcg gccggcaggt tatacatcaa
     2941 cgcgtagtcg atagcccccg cagagaaaga gaagccagca tggataccca gcagtgttgc
     3001 cacaaacagg ctgataccgg tcagcagtgc gtgcaggagg tacagcagcg gtgcaaggaa
     3061 catgaacagg aattccagcg gctcagtcac accggtcagg aacgcagtaa cagcaacaga
     3121 aagcagcatc ccgccaacca tcggacgacg ctctttcggt gctgcgaagt acatcgccag
     3181 cgccgcaccc ggcagaccga acatcataat cgggaagaag ccggacatga acatccccgc
     3241 ggtgccgtca ccggcataga agcggttgat atcaccgtgg aaaaccgtac ccgccgcgtt
     3301 ggtgaattca ccaatctgga accaggcgat ggtgttcagc acctgatgca gaccggttgg
     3361 gatcagcaga cggttgatga aaccaaagat accggaaccc agcgcgcccg cagaaacgat
     3421 ccactcgcct cctgcatgga tagcgtgctg taccggcggc cagacgtaac caaaaatggc
     3481 cgccagcacc aggcagaaga agccggtggc aatcggcaca aagcgtttgc cgccgaagaa
     3541 actcaggaag tccggcagtt taatatcgga ccaacggtta taggctgcgc caccaaccag
     3601 accggtaatg atacccgcca gtacacccat gttaatttct gggttgatgg tcaccatcgc
     3661 tttggttaac acaaagtaac ctaccgcacc cgccagcgcc gccgcaccgg cgctgtcttt
     3721 cgaccagctg gatgccacac cgatggcgaa gattaatgcg aggttatcaa aaatcgcacc
     3781 gcccgcctgg gcaataaacg caacgttaag taaatctggc tgaccgaatc gcagcaacag
     3841 tgccgccacc ggcagcaccg cgatagggag ctgtaacgcc ctaccgagtc gctggaaaaa
     3901 acctaaaata ttcatcttat tccccctacg agaaccctat ttggctcgtt taaagccgta
     3961 tttttatttt gctgcaaatt gtactgccga tgttctgtaa tcagattgtt agatcatctg
     4021 ctacagagtg tgtgaaaatt taattcgtat cgcaaattaa acgcgtgtct tttgtgagtt
     4081 ttgtcaccaa atatcgttat catcactccc ttttactggc taaaccagaa aacttatttt
     4141 atcattcaaa aaatcaggtc ggattgacgc ctgtctgcgc aaatccaggt tacgcttaaa
     4201 gatgcctaat ccgccaacgg cttacatttt acttattgag gtgaataatg agactgatcc
     4261 ccctgactac cgctgaacag gtcggcaaat gggctgctcg ccatatcgtc aatcgtatca
     4321 atgcgttcaa accgactgcc gatcgtccgt ttgttctggg cctgccgact ggcggcacgc
     4381 cgatgaccac ctataaagcg ttagtcgaaa tgcataaagc aggccaggtc agctttaagc
     4441 acgttgtcac cttcaacatg gacgaatatg tcggtctgcc gaaagagcat ccggaaagct
     4501 actacagctt tatgcaccgt aatttcttcg atcacgttga tattccagca gaaaacatca
     4561 accttctcaa cggcaacgcc ccggatatcg acgccgaatg ccgccagtat gaagaaaaaa
     4621 tccgttctta cggaaaaatt catctgttta tgggcggtgt aggtaacgac ggtcatattg
     4681 catttaacga accggcgtct tctctggctt ctcgtactcg tatcaaaacc ttgactcatg
     4741 acactcgcgt cgcgaactct cgtttctttg ataacgatgt caatcaggtg ccaaaatatg
     4801 ccctgactgt cggtgttggt acattgctgg atgccgaaga agtgatgatt ctggtgctgg
     4861 gtagccagaa agcactggca ctgcaggctg cagttgaagg ttgcgtgaac cacatgtgga
     4921 ccatcagctg tctgcaactg catccaaaag cgatcatggt gtgcgatgaa ccttccacca
     4981 tggagctgaa agttaagact ttaagatatt tcaatgaatt agaagcagaa aatatcaaag
     5041 gtctgtaatt gttatccctg ccctggctcc ttgctcaggg caatattttt aaaaatcggg
     5101 ggtcagaatg tatgcattaa cccagggccg gatctttacc ggccacgaat ttcttgatga
     5161 ccacgcggtt gttatcgctg atggcctgat taaaagcgtc tgtccggtag cggaactgcc
     5221 gccagagatc gaacaacgtt cactgaacgg ggccattctc tcccccggtt ttatcgatgt
     5281 gcagttaaac ggctgcggcg gcgtacagtt taacgacacc gctgaagcgg tcagcgtgga
     5341 aacgctggaa atcatgcaga aagccaatga gaaatcaggc tgtactaact atctgccgac
     5401 gcttatcacc accagcgatg agctgatgaa acagggcgtg cgcgttatgc gcgagtacct
     5461 ggcaaaacat ccgaatcagg cgttaggtct gcatctggaa ggtccgtggc tgaatctggt
     5521 aaaaaaaggc acccataatc cgaattttgt gcgtaagcct gatgccgcgc tggtcgattt
     5581 cctgtgtgaa aacgccgacg tcattaccaa agtgaccctg gcaccggaaa tggttcctgc
     5641 ggaagtcatc agcaaactgg caaatgccgg gattgtggtt tctgccggtc actccaacgc
     5701 gacgttgaaa gaagcgaaag ccggtttccg cgcggggatt acctttgcca cccatctgta
     5761 caacgcgatg ccgtatatta ccggtcgtga accgggcctg gcgggcgcga tcctcgacga
     5821 agctgacatt tattgcggta ttatcgctga tggcctgcat gttgattacg ccaacattcg
     5881 taacgctaaa cgccttaaag gcgacaaact gtgtctggtt accgacgcca ccgcgccagc
     5941 aggtgccaac attgaacagt tcatttttgc gggtaaaaca atatactacc gtaacggact
     6001 ttgtgtggat gagaacggta cgttaagcgg ttcatcctta accatgattg aaggcgtgcg
     6061 taatctggtc gaacattgcg gtatcgcact ggatgaagtg ctgcgtatgg cgacgctcta
     6121 tccggcgcgt gcgattggcg ttgagaaacg tctcggcaca ctcgccgcag gtaaagtagc
     6181 caacctgacc gcattcacac ctgattttaa aatcaccaag accatcgtta acggtaacga
     6241 ggtcgtaact caataagaga aagtatgaca ccaggcggac aagctcagat aggtaatgtt
     6301 gatctcgtaa aacagcttaa cagcgcggcg gtttatcgcc tgattgacca gtacgggcca
     6361 atctcgcgga ttcagattgc cgagcaaagc cagcttgccc ccgccagcgt aaccaaaatt
     6421 acgcgtcagc ttatcgaacg cgggctgatc aaagaagttg atcagcaggc ctccaccggg
     6481 ggccgccgcg ctatctccat cgtcaccgaa acccgcaatt tccacgcaat cggcgtacgg
     6541 cttggtcgtc acgacgccac catcaccctg tttgatctca gcagcaaagt gctggcagaa
     6601 gaacattacc cgctgccgga acgtacccag caaacgctgg aacatgccct gttgaatgcc
     6661 attgctcagt ttattgatag ctaccagcgc aaactgcgcg agctgatcgc gatttcggtg
     6721 atcctgccag ggcttgttga cccggacagc ggcaaaattc attacatgcc gcatattcag
     6781 gtagaaaact gggggctggt agaagcactg gaagaacgtt ttaaagtgac ctgtttcgtt
     6841 ggtcacgata tccgtagtct ggcgctggcg gagcactact tcggtgcaag tcaggattgc
     6901 gaagactcca ttctggtgcg tgtccatcgc ggaaccgggg ccgggattat ctctaacggg
     6961 cgcattttta ttggccgcaa cggcaacgtc ggtgaaattg gccatattca ggtcgaaccg
     7021 ctgggtgaac gctgccactg cggcaacttt ggctgcctgg aaactatcgc tgccaacgcc
     7081 gctattgaac aacgggtgtt gaatctgtta aagcagggct accagagccg cgtgccgctg
     7141 gacgactgca ccatcaaaac tatctgcaaa gccgcgaaca aaggcgatag cctggcctcg
     7201 gaagtgattg agtatgtcgg tcgtcatctg ggcaaaacca tcgccattgc tatcaactta
     7261 tttaatccgc aaaaaattgt tattgccggt gaaatcaccg aagccgataa agtgctgctc
     7321 cctgctattg aaagctgcat taatacccag gcgctgaagg cgtttcgcac taatctgccg
     7381 gtggtacgtt ctgagctgga tcaccgctcg gcaatcggcg cttttgcgct ggtaaaacgc
     7441 gccatgctca acggtatttt gctccagcat ttgctggaaa attaatgtgc ttttatagtg
     7501 gcgcttattg ttgtcaatat tctgggtagt ccatgaccat taaaaatgta atttgcgata
     7561 tcgacggcgt gctgatgcac gataacgtcg ccgtaccggg tgcagcggaa tttttgcacg
     7621 ggattatgga taaaggcctg ccgctggtgt tgctgaccaa ctatccttcg cagaccgggc
     7681 aagatctggc gaaccgcttt gccactgccg gtgtcgatgt gccggatagc gtgttttata
     7741 cctctgcgat ggcgactgcc gatttcctgc gtcgccagga aggcaagaaa gcgtatgtgg
     7801 tgggcgaagg cgcactgatt catgaactgt acaaagccgg tttcactatt accgatgtga
     7861 accctgattt tgtgattgtt ggcgaaacgc gttcctacaa ctgggacatg atgcataaag
     7921 cggcctattt cgtcgctaac ggtgcacgct ttatcgccac caacccggac acccacgggc
     7981 gcggttttta tcccgcttgt ggcgcgttgt gtgcagggat tgagaaaatc tccgggcgca
     8041 aaccgttcta tgttggtaag cccagcccgt ggatcatccg cgcggcatta aacaaaatgc
     8101 aggcgcattc ggaagaaacg gtgattgtcg gcgataactt gcgtaccgat attctggcgg
     8161 gcttccaggc gggtctggag acgattctgg tgctttctgg tgtttcgtcg ctcgacgata
     8221 tcgacagtat gccgttccgc ccaagttgga tttacccatc ggtcgctgaa atcgacgtta
     8281 tctgaatagc aaccacgtct gcggacgtgg tttcttattt ttacccatca ttaaaatcat
     8341 cccattcaat aaaacccgtc aaaaattatc gatccatcaa taaatacgcc accacaccct
     8401 atcctttttc acaaatcagc aatcaccatt acgtttttta ttttttcacc gccaaatcgc
     8461 ttgcgcacag tgctgctttg cggcattttt ttaaacaagc aaacacaaca agcaacaaat
     8521 accaagttaa cggagaaggt tatgtgttca atttttggcg tattcgatat caaaacagac
     8581 gcagttgagc tgcgtaagaa agcactcgag ctgtcacgcc tgatgcgtca tcgtggcccg
     8641 gactggtccg gtatttatgc cagcgataac gccattctcg ctcacgaacg tctgtcaatt
     8701 gttgacgtta acgcgggggc gcaacctctc tacaaccaac aaaaaaccca cgtactggcg
     8761 gtaaacggtg aaatctacaa ccaccaggca ttgcgcgctg aatatggcga tcgttatcag
     8821 ttccagacag ggtctgactg tgaagtgatc ctcgcgctgt atcaggaaaa agggccggaa
     8881 tttctcgacg acttgcaggg catgtttgcc tttgccctgt acgacagcga aaaagatgcc
     8941 tacctgattg gtcgcgacca tctggggatc atcccactgt atatgggcta tgacgaacac
     9001 ggtcagctgt atgtggcctc agaaatgaaa tcgctggtgc cagtttgccg cacgattaaa
     9061 gagttcccgg cggggagcta tttgtggagc caggacggcg aaatccgttc ttattatcat
     9121 cgcgactggt tcgactttga tgcagtgaaa gataacgtga ccgacaaaaa cgagctgcgt
     9181 caggctctgg aagattccgt taaaagccat ctgatgtctg atgtgcctta cggtgtgctg
     9241 ctttctggtg gtctggattc ctcaattatt tccgctatca ccaagaaata cgcagcccgt
     9301 cgcgtggaag atcaggaacg ctctgaagcc tggtggccgc agttacactc ctttgctgta
     9361 ggtctgccgg gttcaccgga tcttaaagca gcccaggaag tggcaaacca tctgggcacg
     9421 gtgcatcacg aaattcactt cactgtacag gaaggtctgg atgccatccg cgacgtgatt
     9481 taccacatcg aaacttatga tgtgaccact attcgcgctt caacaccgat gtatttaatg
     9541 tcgcgtaaga tcaaggcgat gggcattaaa atggtgctgt ccggtgaagg ttctgatgaa
     9601 gtgtttggcg gttatcttta cttccataaa gcgcccaacg ctaaagaact gcatgaagag
     9661 acggtgcgta aactgctggc cctgcatatg tatgactgcg cgcgcgccaa caaagcgatg
     9721 tccgcctggg gcgtggaagc gcgcgttccg ttcctcgaca aaaaattcct tgatgtggcg
     9781 atgcgtatta acccacagga taaaatgtgc ggtaacggca aaatggaaaa acacatcctg
     9841 cgtgaatgtt ttgagtcata cctgcctgca agcgtggcct ggcggcagaa agagcagttc
     9901 tccgatggcg tcggttacag ttggatcgac accctgaaag aagtggcggc gcagcaggtt
     9961 tctgatcaac agctggaaac tgcccgcttc cgcttcccgt acaacacgcc gacgtccaaa
    10021 gaagcgtatc tgtaccggga gatctttgaa gaactgttcc cacttccgag cgccgctgag
    10081 tgcgtaccgg gcggtccttc cgtcgcgtgt tcttccgcta aagcgattga gtgggatgag
    10141 gcgttcaaga aaatggacga tccgtctggt cgtgcggttg gcgttcacca gtcggcgtat
    10201 aagtaagaaa tgcgaaacag ccccggtgaa atccggggct gtttcagtta ttgaggtgct
    10261 tgttaattct gatatgcagt aaggaaatgt tccgggtttg ccacatcact gtttgacaac
    10321 tcatcactgt aggccagtat ttcatagatt aatttcccac cactattgtt gaattccgaa
    10381 tttcatcctg tacgacaaaa gaatggctgt agggtgggga aaaaacacaa agtaaacaat
    10441 aattgacgaa tatagcgcca cgctgttcgc aacctaacca aacagtcact ttcgagcaat
    10501 tttccttgaa aaagaggttg acgctgcaag gctctatacg cataatgcgc cccgcaacgc
    10561 cgataaggta tcgcgaaaaa aaagatggct acgtagctca gttggttaga gcacatcact
    10621 cataatgatg gggtcacagg ttcgaatccc gtcgtagcca ccatcttttt tgcgggagtg
    10681 gcgaaattgg tagacgcacc agatttaggt tctggcgccg caaggtgtgc gagttcaagt
    10741 ctcgcctccc gcaccattca ccagaaagcg ttgtacggat ggggtatcgc caagcggtaa
    10801 ggcaccggtt tttgataccg gcattccctg gttcgaatcc aggtacccca gccatcgaag
    10861 aaacaat
//
LOCUS       DAPHDW010000149        10828 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.151, whole genome shotgun sequence.
ACCESSION   DAPHDW010000149 DAPHDW010000000
VERSION     DAPHDW010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.151"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..1667
                     /gene="yeaV"
                     /locus_tag="RH057_003771"
     CDS             57..1667
                     /gene="yeaV"
                     /locus_tag="RH057_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter YeaV"
                     /protein_id="HDV3130075.1"
                     /translation="MMSNVKKKDVPLISISLVAILFIAAALSLFPQQSADAANAIYTF
                     VTRTLGSAVQVLVLLAMGLVIYLATSKYGNIRLGEGKPEYSTLSWLFMFICAGLGSST
                     LYWGVAEWAYYYQTPGLNIAPRSQQALEFSVPYSFFHWGISAWATYTLASLIMAYHFH
                     VRKNKGLSLSGIIAAITGVRPQGPWGKLVDLMFLIATVGALTISLVVTAATFTRGLSA
                     LTGLPDNFTVQAFVILLSGGIFCLSSWIGINNGLQRLSKMVGWGAFLLPLLVLIVGPT
                     EFITNSIINAIGLTTQNFLQMSLFTDPLGDGSFTRNWTVFYWLWWISYTPGVAMFVTR
                     VSRGRKIKEVIWGLILGSTVGCWFFFGVMESYAIHQFINGVINVPQVLETLGGETAVQ
                     QVLMSLPAGKLFLAAYLGVMIIFLASHMDAVAYTMAATSTRNLQEGDDPDRGLRLFWC
                     VVITLIPLSILFTGASLETMKTTVVLTALPFLVILLVKVGGFIRWLKQDYADIPAHQV
                     EHYLPQTPVEALEKTPVLPAGTVFKGDN"
     gene            1699..2823
                     /gene="yeaW"
                     /locus_tag="RH057_003772"
     CDS             1699..2823
                     /gene="yeaW"
                     /locus_tag="RH057_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
                     /protein_id="HDV3130076.1"
                     /translation="MSNLSPDFVLPENFCANPQEAWTIPARFYTDQNAFEHEKENVFA
                     KSWICVAHSSELANANDYVTREIIGESIVLVRGRDKVLRAFYNVCPHRGHQLLSGEGK
                     AKNVITCPYHAWAFKLDGNLAHARNCENVANFDSDKAQLVPVRLEEYAGFVFINMDPN
                     ATSVEDQLPGLGAKVLEACPEVHDLKLAARFTTRTPANWKNIVDNYLECYHCGPAHPG
                     FSDSVQVDRYWHTMHGNWTLQYGFAKPSEQSFKFEEGTDAAFHGFWLWPCTMLNVTPI
                     KGMMTVIYEFPVDSETTLQNYDIYFTNEELTDEQKSLIEWYRDVFRPEDLRLVESVQK
                     GLKSRGYRGQGRIMADSSGSGISEHGIAHFHNLLAQVFKD"
     gene            2879..3844
                     /gene="yeaX"
                     /locus_tag="RH057_003773"
     CDS             2879..3844
                     /gene="yeaX"
                     /locus_tag="RH057_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HDV3130077.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHII
                     VQMSDGDNQYSNAYSLLSSPHDTSCYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIAGGIGITPFLSHMAELQHSDVDWQLHYCSRNPESCAFRDELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(3898..5013)
                     /gene="rnd"
                     /locus_tag="RH057_003774"
     CDS             complement(3898..5013)
                     /gene="rnd"
                     /locus_tag="RH057_003774"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HDV3130078.1"
                     /translation="MITTDDALASLCEAVRAFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEEALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(5095..6780)
                     /gene="fadD"
                     /locus_tag="RH057_003775"
     CDS             complement(5095..6780)
                     /gene="fadD"
                     /locus_tag="RH057_003775"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HDV3130079.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGMIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            complement(6985..7566)
                     /gene="yeaY"
                     /locus_tag="RH057_003776"
     CDS             complement(6985..7566)
                     /gene="yeaY"
                     /locus_tag="RH057_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HDV3130080.1"
                     /translation="MAVHKNVIKGILAGTFALMLSGCVTVPDAIKGSSPTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            complement(7606..8301)
                     /gene="tsaB"
                     /locus_tag="RH057_003777"
     CDS             complement(7606..8301)
                     /gene="tsaB"
                     /locus_tag="RH057_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HDV3130081.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLAAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQIFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            complement(8359..10269)
                     /locus_tag="RH057_003778"
     CDS             complement(8359..10269)
                     /locus_tag="RH057_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HDV3130082.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDNRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            10401..10745
                     /locus_tag="RH057_003779"
     CDS             10401..10745
                     /locus_tag="RH057_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HDV3130083.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
BASE COUNT         2564 a         2911 c         2731 g         2622 t
ORIGIN      
        1 aaataattac aactggcaaa agccagagac gggcttttat gcccaggaga tgactcatga
       61 tgagcaatgt taagaaaaaa gatgtgccgc tgataagcat cagcctggtg gccattcttt
      121 tcatcgcagc tgcattaagc cttttcccac aacaatcggc cgacgcggcc aacgcaatat
      181 acacttttgt tacgcgtacg ttaggttccg ccgtacaggt attggttttg ctggcaatgg
      241 gactggtgat ttatttagcc accagtaaat acggcaatat tcgtcttggc gaaggaaaac
      301 cggaatacag cacgctctcc tggctgttta tgtttatttg tgccggttta ggttcttcta
      361 cgctttattg gggggttgct gaatgggcct attattatca aacgcctgga ttaaatatcg
      421 caccgcgttc acaacaggca ctcgaattta gcgtccctta ctccttcttc cactggggca
      481 tcagcgcctg ggcaacttat acgctggcct cattaatcat ggcttatcac tttcatgtgc
      541 gtaaaaacaa aggtctgagt ctttccggca ttattgccgc gattaccggc gttcgcccgc
      601 aaggcccatg gggaaaactg gttgatttga tgttcctgat cgccactgtc ggcgcactga
      661 ctatttccct tgttgtcacc gcagcaacct ttactcgtgg actttccgcg ctgaccggtt
      721 tacccgataa cttcaccgtg caggcatttg tgatcctgct ttccggcggc attttttgcc
      781 taagctcgtg gattggtatc aacaacggtt tgcaacgtct gagcaaaatg gttggctggg
      841 gcgcgttcct gctgccatta ctggtgctga ttgtcggccc aaccgaattt attaccaaca
      901 gcatcatcaa tgccatcggt ctgaccacgc aaaacttcct gcaaatgagc ttattcaccg
      961 atccgcttgg cgatggttca tttacccgca actggaccgt tttctactgg ctgtggtgga
     1021 tctcatacac ccctggcgta gcaatgtttg tcacccgcgt ttcccgcggt cgtaagatta
     1081 aagaagttat ctggggactg atcctcggca gcaccgtcgg ttgctggttc ttcttcggcg
     1141 taatggaaag ctatgccatt catcagttta tcaatggcgt aatcaacgtc ccacaggtgc
     1201 tggaaacact gggcggcgaa acagctgtac agcaagttct gatgtcgttg ccagccggta
     1261 aattgttcct cgccgcatac ctgggcgtga tgattatttt ccttgcctcg catatggatg
     1321 cggtggccta caccatggct gcgaccagta cgcgtaatct ccaggaaggt gacgatcctg
     1381 accgtgggct gcgtcttttc tggtgcgtgg tgatcactct gatcccgctt tccatcttgt
     1441 ttaccggtgc ttcgctggaa acgatgaaaa ccaccgtcgt gctcacagcc cttcccttcc
     1501 tcgtcatttt actggtgaaa gtcggcggat ttattcgctg gctgaaacag gattacgccg
     1561 acattccggc tcatcaagtt gaacattatc tcccgcagac accggttgaa gccctggaaa
     1621 aaacgccagt gctccctgcg ggaaccgtat tcaaaggcga caactgagcg ccatcatcct
     1681 aacgataaag gtatccctat gagcaatctg agccctgact ttgtactacc cgaaaatttt
     1741 tgcgctaacc cgcaagaggc gtggaccatt cctgcccgtt tttataccga tcagaacgcg
     1801 tttgaacacg aaaaagagaa cgtcttcgcc aaaagctgga tttgcgtcgc tcacagcagc
     1861 gaactggcga atgccaatga ttatgtgacg cgtgagatca ttggcgaaag catcgtgctg
     1921 gtacgcggtc gtgataaggt tttgcgcgcg ttctataacg tgtgtccgca ccgtggtcat
     1981 cagttgttga gcggtgaagg aaaagcaaaa aatgtgatta cctgcccgta tcacgcatgg
     2041 gcattcaaac tcgatggcaa tctggcccat gcacgtaact gcgaaaacgt cgccaatttc
     2101 gatagcgaca aagcgcaact ggttccggtg cgtctggaag aatatgccgg attcgtcttc
     2161 atcaacatgg accccaacgc caccagcgta gaagatcagt tacccggtct gggcgcgaaa
     2221 gtgctggaag cctgcccgga agtccacgat ctgaaactgg cggcccgctt taccacccgc
     2281 acgcctgcca actggaagaa cattgtcgat aactatctcg agtgctatca ctgtggtccg
     2341 gcgcatccag gtttctccga ctccgtacag gttgatcgtt actggcacac catgcacggt
     2401 aactggacgc tgcaatacgg tttcgccaaa ccgtccgaac agtcgtttaa atttgaagag
     2461 ggtacggatg cggcattcca cggtttctgg ctgtggccgt gcacgatgct gaacgtcacc
     2521 ccgatcaaag ggatgatgac ggtcatttat gaattcccgg tggattctga aactaccctg
     2581 caaaactacg atatttactt caccaatgaa gagttaaccg acgagcaaaa atcgctgatt
     2641 gagtggtatc gcgatgtgtt ccgtccggaa gatttacgtc tggttgaaag cgtacagaaa
     2701 gggctgaaat cgcgtggcta tcgtggtcag gggcgcatca tggccgacag tagcggtagt
     2761 ggcatttccg aacatggtat cgcccatttc cataatctgc tggcgcaggt gtttaaggac
     2821 taatgacatc ggcggcggta ttttccgccg ctgggctgat ttttgatgga gtacagcaat
     2881 gtcagactat caaatgtttg aagtacaggt gagtcaggtg gaaccgctta ccgaacaggt
     2941 gaaacgcttc acgctggtgg caaccgatgg caaaccgctg cctgcgttca ccggaggaag
     3001 tcacatcatt gtgcagatga gcgatggtga taaccagtac agcaatgcgt attcactact
     3061 gagttcgccg catgacacct catgttatca gattgccgtt cggctggagg aaaactcgcg
     3121 cggcggttcc cgctttttgc atcagcaggt aaaagtgggc gatcggttaa cgatttcaac
     3181 gcctaataac ctgtttgcgc taattccctc agccagaaag catctgttta tcgcgggcgg
     3241 tattggtatc acccctttcc tgtcgcacat ggcagagctg caacacagcg acgtcgactg
     3301 gcagctacat tactgctcgc gaaatccaga aagttgcgca tttcgtgatg agctagtcca
     3361 gcatccgcag gctgagaaag tccatttgca tcattcatca accggaacac gactggaatt
     3421 agcgcgatta ttggcggata tcgaacctgg cacacacgtt tatacctgtg gccccgaggc
     3481 gctaattgaa gcggtaagaa gtgaagctgc gcgtctggac atcgccgccg atacgctgca
     3541 ctttgagcaa tttgctatcg aagacaaaac cggcgatgca tttaccctgg tgcttgcccg
     3601 ttccggaaaa gagtttgtgg tgccggaaga gatgactatt ttgcaggtta ttgaaaataa
     3661 taaagccgcg aaagtggaat gtttatgtcg tgaaggggta tgcggaacct gcgaaacagc
     3721 aatactggaa ggtgaagctg accatcggga tcaatatttt agcgatgaag agcgtgccag
     3781 ccagcaaagt atgttgatct gttgttcgcg tgcgaagggt aaacgcctgg tgttggattt
     3841 gtagtttgcc gaagccggat gtggcgctga gcgcgcccag tccggcttcg gaagatttta
     3901 ctgcggatat tcctgcaata aattgtgtaa tgcttccgcc atcagctcac cacgccagcc
     3961 ggaaatcagc tccggcaaat tgttctgcgg tttcagtttc cagtgccagt tcagcagttg
     4021 gttgatttgc cgacgcgatg ccagcaattc ggcgctgatc ttatgcgttt cgctcacgtc
     4081 agtaatcagc gacttaatcg ctttaaacgc tttacgataa cccggcatgt ccatcaggtt
     4141 aagcatcggc tgcggtaagg cctcttccgg caatgtctgc gctttttcca ccagcgccag
     4201 cagcgtttta ccgtgaaagc ggatttcgct accggataaa cccaggctgt ccagttcgcc
     4261 taaacttccc ggcatataac gcgctaccga ccacaaatgc tcttcacgca cgacaaagtt
     4321 caccgccaga tcgcgctctc gcgccttgcg cagtcgccag tcggctaaca gttgcagaca
     4381 ggccagttgg cgtgtgcgta attgccaggc attggtgata tcacgccagg catcttccgg
     4441 cgcaacgact tcctgacgac gcatttgcat caggcggcat tcatccagcg ccgcaggtag
     4501 ccagccggag gcctccgttt ctaccataag cttggcggtg atcggtaaca gataccagac
     4561 atccgccgct gcgtattcac actgacgttc ggtcagcggt ctggccagcc agtcggtgcg
     4621 cgattcactc ttgtccagcg taacgccgga atactcttcc accatggaag cgaaacccca
     4681 tgacatcggg cgtccgcaga aggcagcaag gatttgcgtg tcaatcaagg gttgtggtaa
     4741 ttcgccaaag acattgagga acacttccag atcttcactg cctgcatgga gaaattttgt
     4801 gatggacgga tcgcgcagga tcgctttcag cggtgaccag tcggtgatcc cgagtggatc
     4861 gattagcgcc agatgctcgc catcgaaaag ttgaatcaac cccagctgcg ggtaataagt
     4921 gcgcgtacga acaaattcag tatccagggc tatcgccgga aaggcacgga cggcttcaca
     4981 caaagaagcc agcgcatcgt ccgtggtaat catttggtaa ttcaaattgt tttctcttta
     5041 gtgggcgtca aaaaaaacgc cggattaacc ggcgtctgac gactgactta acgctcaggc
     5101 tttattgtcc actttgccgc gcgcttcgtc acgtaattct cgtcgcaaaa tttttccgac
     5161 gttagatttc ggtaactcat cacgaaactc caccagcttc ggtactttgt atcccgtgag
     5221 ctgacggcgg caaaaagtca ccagtgactc ttcggtaagc gatggatctt ttttcactac
     5281 gaagattttc accgcttcac cactggagcc ggaaggtacg ccaacagccg cgacttcctg
     5341 tacgccagga tgctgcatga cgacatcttc aatctcgttg ggatagacgt taaaaccgga
     5401 aaccagaatc atgtcttttt tacgatcgac aatgcgcagg aatccttctt catccattac
     5461 cgcgatgtcg ccggtgtgta accagccatt tttgatgatt tcatcggtag catcgggacg
     5521 ctgccagtaa cccagcatca cctgcggtcc tttgacacaa agctcacccg gttgacctgg
     5581 tggtacttca ttatcatcat catccaccag tttggcttcc gtcgacggca ccggcaaacc
     5641 gatgctacca ctatgataat caatatcata tgggttaacg ctgaccagcg gcgcacactc
     5701 ggtaaggcca tagccttcca gcagatactg tccggtcagt ttcacccaac gctctgccac
     5761 cacttgctgc actggcatcc caccgcctgc ggaaagatgc agactggaga aatccagctg
     5821 ctggaactct ttattgttca gcaacgcatt gaacaaggtg ttaacgccag tgatagcggt
     5881 aaacggatat ttcgctaact cttttaccaa ccctggaata tcacgcgggt tagtgataag
     5941 caggttctgc ccacccagtt cgataaacag caggcagtta atggtcaggg caaaaatgtg
     6001 atacagcggc agtgccgtta ctaccagttc tttacctgga tgcagcaacg gcccataggt
     6061 ggcgttgacc tgttccaggt tcgccagcat attgcggtga gtcagcatcg cgcctttcgc
     6121 cacacctgtg gtgccgccgg tgtattgcag gaaggctaaa tcttccggca ccagttcggg
     6181 tttgacgtac tgcatccggt agccgttatg cagtgcgcta cgaaatgaaa tggcatctgg
     6241 cagatggtat ttcggcacca aacgcttgat gtatttaaca acgaaattga ctaccgtgcc
     6301 ttttgccgta gatagctgat cgcccatacg ggtcagaatt acgtgctgaa cggcggtttt
     6361 atcaaccact ttttccagtg tgtgagcaaa gttagacacg ataacaatcg ccgatgcgcc
     6421 gctatcgtta agctgatgct caagctcacg cggggtatac aacgggttaa cgtttacgac
     6481 gatcatcccg gcacgcaaaa tgccaaacag cgccaccgga tattgcaata aattaggcat
     6541 catcaacgca acgcgatcgc ctttcttcag ccccaaccct tgttgcaaat aagcggcaaa
     6601 cgcgcgactg cgttcttcca gcttgcggaa ggtcattacc tcccccatat tcacaaacgc
     6661 aggctgatcg gcgtagcgcg cgaccgactg ctcaaacata tctaccagag attgataacg
     6721 gtcagggttg atctccgtcg gaacgtccgc gggataacgg ttaagccaaa ccttcttcaa
     6781 ttcttcacct ctaaaatgcg tgttcgtcgt catcgcaacc ccaaatgata tacatgccgt
     6841 taacataata ttaactcatc ataccagctt gataattacc caacgaaaag gttgcgaagc
     6901 gcgtcactat ttatttttat ctttaccgta agaatgcaga aacagcggac cagccgctgt
     6961 ttctttttct ttaaaaacaa gcaattattc agttacaact gtttgtactc tcgcggggcc
     7021 gggattatac cagccccatc cgccgtagcc atagggccag ccacgaccgc cataaaacca
     7081 tggatcaatc ggctgaggcg gcataatcac ctgctgggtt aaatgccaac gtttgtaacc
     7141 cgttacttgc atcaccataa atttataggg cgtattgccg attttgccgt caaccgcacc
     7201 agtgattggc ccgactaccg taaccagttg tccacggaaa tccaccgggt ccagaaaacc
     7261 gttcacatcg gcataaatgc gaccgcgaga aggttctccc agcgtcggtc tggctccgct
     7321 gtccagcggt acggtagcaa tttccaggcg ggttttccct tgctggtttt gtaccgcaac
     7381 cactttgcca ccaaagcgtg cctcctgacc aacgtacagc tgcggcgcac tcatcacccg
     7441 aactaaatct tgttgcggcg tgggactgct gcctttaatg gcgtccggca cagtgacaca
     7501 accgctcagc attagcgcaa acgtacctgc cagtatgcct ttgataacat tcttatgaac
     7561 cgccatgatg cgactccttt ttttctcagg gcatactctt aagattcatt ctttgcccgg
     7621 aagtttcttc catgcgacgt tgttacgcaa ataaaccggc tcggcatgtt ccaccgccac
     7681 cgttttaccc tcggcaaata tctgacaagc aatcggcagc atatcttctg cagcgggcag
     7741 taacacttct ccgtcgcgca aaaccagccc gctctcttta ccgagatccg gccaggcttg
     7801 ccagcccgtc cctaccgtta cccattcgcc ggaaagctgt tgcattcgtt catgaacgag
     7861 ttcgggtttg agtacggctt cggtttcttc accgtgccag ataccgtttt catcacgctg
     7921 atattcggcc cagtaaactt cgcccattcg cgcgtcaatg gctgccagca cgcgggttgc
     7981 gccgtttttg cgccacgccc cttgcgccat cgtcattagc gtggagacgc caatcatcgg
     8041 tagctctgcg ccaagtgcca gcccttgcgc aatgccaata ccaatgcgca cgccggtaaa
     8101 gctaccgggg ccgcgcccgt aagccagagc gttaatatca gtcagggaag ttccgctggt
     8161 ggtcaggata tcctgcacca tcggtaagat tcgttgagta tgttcacgag ggcaaagctc
     8221 aaaatgagcg ttgacagtac cgtcgttcca cagggcgaca gagcaggcct ctgtcgcggt
     8281 atcgatagcc agaattcgca tgggtcttcg tgcttagatc aataaaaagg cgcgcatcat
     8341 accatactcc gtaacaaatt acctggagga tggtatcgca aggaaacgaa ccgcacgggc
     8401 aatgtcacga gtgcgtggcg cgggcggcag actggcgaga aacgtcgcgc cgtaaggacg
     8461 catcaccagc cgattgtcac aaatcaccag cacgccacga tcgtcggcgt cgcgaatcag
     8521 tcgccctacc ccctgtttga gagtaatgac ggcatctggt agttgcactt catcgaacgg
     8581 gtcgccaccg cgcaaacgac aatcttccat gcgcgctttt aacagtggat catccggcga
     8641 ggtaaacggc aacttgtcga taatcaccaa tgacaatgta tcgccacgca cgtccacccc
     8701 ttcccagaaa ctactggttg ccacaagaag cgcgttaccg gcgctgacaa attgctgcaa
     8761 cagttgccct ttgctggttt ccccctgcaa caaaacggga agcgtcatgg tagcgcggaa
     8821 ctgctcggcc agatcacgca tcatggcgtg cgaggtacaa agcataaaac aacgaccgtt
     8881 gttagcttcg atgatcggtc gcagcattgc cgctaactgg cgagcagaac ctggctggtt
     8941 ggtttgcggc agattgcgcg gcacacagag taacgcctgg cggctgtaat caaacgggct
     9001 tggcaatagc aacgactccg cctgttctat cccaagccgc gaggtgaaat gatgcagatc
     9061 gtcgttcacc gacagcgttg ctgaggtgaa gatccagcta ccgggctttt gcgccattaa
     9121 ctctttgaat ttatccgcca cgctgagcgg cgtgagagcc agagtaaaat ggcgcgaagt
     9181 gcattcgtac cagtagctgt agcccggctg attgatctct tttagccgct tcagccgtgt
     9241 gcgatacaac gtggcgcgct caaatgccgc atccagcaag gcggaacgcc ccagtgacag
     9301 tttcgccacg tcataacaaa gttccagggt gtcatcgagc agtaaaaatg cccgctgaat
     9361 ttgcggatta gctaacagct cacgcaggtt accgcgataa cctggctcac cgagttgcag
     9421 acgaaaatcc tgcgcactct gggcaagacg atcagcgcac ttttgtaact gctgggtgtc
     9481 ttttaattcg gtgcggtagg cgatggtgat gtcttttgcc aggtcgagca gttgtcgact
     9541 ggagagtgac tgaccaaaat actggctggc aatgtccggt agctggtggg cttcgtcgaa
     9601 gatcatgacg tccgcttccg ggatcaactc gccaaatcca ctctctttaa ccaccatatc
     9661 cgccagaaag agatgatggt ttaccaccac cacatcggcg tccatcgctt ttttacgtgc
     9721 tttgaccaca aagcaatctt tatacatcgg gcagtcgctg ccaagacagt tgtcgttggt
     9781 gctggtgacc agcggccacg cctgtgaatc ttccgccacg ctgacgcagg tgctgatatc
     9841 accatcgact gtttgattag accaggagcg cagcaggatc acatcgctta agatttgtac
     9901 cggcagatcg ccccccgcca gcgcctgctg ttcgagacgt tcaaggcaga ggtagtttga
     9961 gcgccctttc agcagcgcca cgttgcccgt gtatttcaac gcctttgaga ctgttggcaa
    10021 atcgcggctg tagagctgat cctgcaacgc ttttgagccg gtcgagataa tgactttctt
    10081 tttcgcccgc agcgcaggag ccaggtaagc gtaggttttg cccgtaccgg ttcctgcttc
    10141 caccaccagc ggctggcctt tttctatcgc ctgggtgacg gctaccgcca tctgtcgctg
    10201 tggttctcgc ggcttaaagc ctggtatcgc tttcgccagc tgaccgtctg gtgcaaaatc
    10261 gtccgtcaca ctaccccctg ttgatttgaa cagggattat gtcaggatga ggtcgctttc
    10321 gccagttgaa gtggtgacgg cgacctcaca ttgtggcagt ctttgcagca cgaaaatgga
    10381 aggataatga ggacaaaata atgactatcg ttcgtatcga tgctgaagcc cgctggtctg
    10441 atgtagtaat ccacaacaac acgctctact acactggtgt accggaaaac ctcgacgccg
    10501 atgcctttga gcaaaccgcc aacacgctgg cgcagattga cgccgtgctg gaaaaacagg
    10561 gcagcaataa atcgagcatt ctggatgcca ccattttcct ggccgataaa aacgacttcg
    10621 cggcgatgaa taaagcgtgg gatgcctggg ttgtcgcggg tcatgcgccg gtgcgctgca
    10681 cggtacaagc gggtttgatg aacccgaagt ataaagttga aattaagatt gtggctgcgg
    10741 tgtaagcttt atcgaagcaa aataagtcag acgataattt atcgataata ctggtcggtt
    10801 ttacataaat cgaccagaga ttaagata
//
LOCUS       DAPHDW010000150        10721 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.152, whole genome shotgun sequence.
ACCESSION   DAPHDW010000150 DAPHDW010000000
VERSION     DAPHDW010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.152"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            72..1214
                     /locus_tag="RH057_003780"
     CDS             72..1214
                     /locus_tag="RH057_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3130084.1"
                     /translation="MECDLLMIKIEKVINKNDLKAFIAFPSSLYPDDPNWIPPLFIER
                     NEHLSAKNPGTDHIIWQAWVAKKAGQIVGRITAQIDTLHRERYGEDTGHFGMIDAIDD
                     PQVFAALFGAAEAWLKSQGASKISGPFSLNINQESGLLIEGFDTPPCAMMPHGKPWYA
                     AHIEQLGYHKGIDLLAWWMQRTDLTFSPALKKLMDQVRKKVTIRCINRQRFAEEMQIL
                     REIFNSGWQHNWGFVPFTEHEFATMGDQLKYLVPDDMIYIAEIDSAPCAFIVGLPNIN
                     EAIADLNGSLFPFGWAKLLWRLKVSGVRTARVPLMGVRDEYQFSRIGPVIALLLIEAL
                     RDPFARRKIDALEMSWILETNTGMNNMLERIGAEPYKRYRLYEKQI"
     gene            complement(1221..1985)
                     /gene="glcC"
                     /locus_tag="RH057_003781"
     CDS             complement(1221..1985)
                     /gene="glcC"
                     /locus_tag="RH057_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HDV3130085.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSRVARLNRVQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENNKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNLS"
     gene            2236..3735
                     /gene="glcD"
                     /locus_tag="RH057_003782"
     CDS             2236..3735
                     /gene="glcD"
                     /locus_tag="RH057_003782"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
                     /protein_id="HDV3130086.1"
                     /translation="MSILYEERLDGALPDVDRTSVLMALREHVPGLEILHTDEEIIPY
                     ECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLEKGV
                     LLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIGGNV
                     AENAGGVHCLKYGLTVHNLLKIEVQTLDGEALTLGSDALDSPGFDLLALFTGSEGMLG
                     VTTEVTVKLLPKPPVARVLLASFDSVEKAGLAVGDIIANGIIPGGLEMMDNLSIRAAE
                     DFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAGATDVRLAQDEAERVRFW
                     AGRKNAFPAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVFHAGDGNM
                     HPLILFDANEPGEFARAEELGGKILELCVEVGGSISGEHGIGREKINQMCAQFNSDEI
                     TTFHAVKAAFDPDGLLNPGKNIPTLHRCAEFGAMHVHHGHLPFPELERF"
     gene            3735..4787
                     /gene="glcE"
                     /locus_tag="RH057_003783"
     CDS             3735..4787
                     /gene="glcE"
                     /locus_tag="RH057_003783"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HDV3130087.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGAGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMNEIAQWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVAGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTAEDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            4798..6021
                     /gene="glcF"
                     /locus_tag="RH057_003784"
     CDS             4798..6021
                     /gene="glcF"
                     /locus_tag="RH057_003784"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HDV3130088.1"
                     /translation="MQTQLTEEMRQNARALEADSILRACVHCGFCTATCPTYQLLGDE
                     LDGPRGRIYLIKQVLEGNEVTLKTQEHLDRCLTCRNCETTCPSGVRYHNLLDIGRDIV
                     EQKVKRPLPERMLREGLRQVVPRPAVFRALTLVGLVLRPFLPEQVRAKLPAETVKAKP
                     RPPLRHKRRVLMLEGCAQPTLSPNTNAATARVLDRLGISVMSANEAGCCGAVDYHLNA
                     QEKGLARARNNIDAWWPAIEAGAEAILQTASGCGAFVKEYGQMLKNDALYADKARQVS
                     ELAVDLVELLREEPLEKLAVRGDKKLAFHCPCTLQHAQKLNGEVEKVLLRLGFTLTDV
                     PDSHLCCGSAGTYALTHPDLARQLRDNKMNALESGKPEMIVTANIGCQTHLASAGRTS
                     VRHWIEIVEQALEKE"
     gene            6026..6430
                     /locus_tag="RH057_003785"
     CDS             6026..6430
                     /locus_tag="RH057_003785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein GlcG"
                     /protein_id="HDV3130089.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRETKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKAAAAVLAK"
     gene            6452..8623
                     /gene="glcB"
                     /locus_tag="RH057_003786"
     CDS             6452..8623
                     /gene="glcB"
                     /locus_tag="RH057_003786"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HDV3130090.1"
                     /translation="MSQTITQGRLRIDANFKRFVDEEVLPGTGLDAAAFWRNFDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAALTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRHYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHQTNVQSVQANIAQTEFN
                     AEFEPLLDDLLTIPVAENANWSVEEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNADDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            8979..10661
                     /gene="glcA"
                     /locus_tag="RH057_003787"
     CDS             8979..10661
                     /gene="glcA"
                     /locus_tag="RH057_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate permease GlcA"
                     /protein_id="HDV3130091.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGAPVAITGALLVGLGFKPLYAAGLC
                     LIANTAPVAFGALGVPILVAGQVTGIDPFHIGAMAGRQLPFLSVLVPFWLVAMMDGWK
                     GVKETWPAALVAGGSFAVTQFFTSNYIGPELPDITSALVSIVSLALFLKVWRPKNTET
                     AISMGQSAGAMVVNKPSSGGPVPSEYSLGQIIRAWSPFLILTVLVTIWTMKPFKALFA
                     PGGAFYSLVINFQIPHLHQQVLKAAPIVAQPTPMDAVFKFDPLSAGGTAIFIAAIISI
                     FILGVGIKKGIGVFAETLISLKWPILSIGMVLAFAFVTNYSGMSTTLALVLAGTGVMF
                     PFFSPFLGWLGVFLTGSDTSSNALFGSLQSTTAQQINVSDTLLVAANTSGGVTGKMIS
                     PQSIAVACAATGMVGRESELFRYTVKHSLIFASVIGVITLLQAYVFTGMLVS"
BASE COUNT         2484 a         2707 c         3022 g         2508 t
ORIGIN      
        1 gattattaag gtgtcgttcg ttgctgttgc aacctgtttt tcaccgttaa ttatttgtta
       61 tccacagaga tatggaatgc gatctgctga tgattaaaat tgaaaaagta attaataaaa
      121 atgaccttaa ggcatttatc gcttttccgt catcacttta tcccgacgat ccaaactgga
      181 tacccccttt attcatagaa cgcaacgagc atttgtctgc gaaaaatccg gggacggacc
      241 atatcatctg gcaggcgtgg gtggcaaaaa aagcggggca gatagttggg cgtattaccg
      301 cgcaaataga taccttgcac cgtgagcgtt acggtgaaga taccggtcat ttcggcatga
      361 ttgacgccat tgatgatccg caggtttttg ccgcgttgtt tggcgcagcg gaagcgtggt
      421 tgaagtcaca aggtgcaagt aagatcagcg gtcctttcag cctgaatatc aatcaggaaa
      481 gcggattact gattgaaggt tttgacacac caccctgtgc gatgatgcca cacggcaaac
      541 cgtggtatgc cgcgcatatt gaacaactgg gttatcacaa aggcattgat ttactggcgt
      601 ggtggatgca gcgaaccgat ctcactttct ctccggcgct aaaaaaactg atggatcagg
      661 tgcgcaaaaa ggtgaccatt cgctgcatca atcgtcagcg gtttgccgaa gaaatgcaga
      721 tcctgcgtga gattttcaac tctggctggc agcacaactg gggatttgtg ccgtttaccg
      781 aacatgaatt cgcgaccatg ggcgatcaac ttaaatatct ggtgccggac gatatgatct
      841 atatcgctga gattgattct gcaccctgcg cgtttattgt cggcttgccg aacatcaacg
      901 aggcgattgc cgatctgaac ggatcgctct ttcccttcgg ctgggcaaaa ttgctgtggc
      961 gtttgaaagt cagcggtgtg cgaactgcgc gagtaccgct gatgggcgtg cgcgacgagt
     1021 atcagttcag ccgcatcggc ccggtgattg cgctgttatt gattgaagcc ttacgcgatc
     1081 cgtttgcccg ccggaagatc gatgcgctgg agatgtcatg gatcctcgaa accaatacag
     1141 gcatgaataa catgctggaa cgcattgggg cggagccgta caagcgttac cgattgtatg
     1201 aaaagcagat ttaataccag ctaactcagg ttcatctcca gcggcaccgc cgagcgaatc
     1261 aaatggtggc cttccagctc gatatcgtgg agattctttt tcacggtccg cacatgatcg
     1321 cgtgctgcgc gctgggcgac gtgcggcagc cgctgcaaca ccgcgttgta gatcctcgca
     1381 tgctggcgat cgatctgctt tttttgctgt ggtcgatggt agagattatt tacgctggca
     1441 aacactgaat taaacatcag atcggtcaat gattgcagcg taaacaccag cacctgattg
     1501 tgagaagcct gacaaatggc gagatggaaa gcgtgatcca actgcgcatg ttcgatcagc
     1561 gaaatctctt tgttgttctc actggcggcg agcatttttt cataacagcg ggttatcaca
     1621 acaaaatcag cctgcgttcc cagcgttgcc gccagccttg ccgattcgcc ctccagtaat
     1681 gcgcgaacgt cgagcagatc gtacagcgtt cgcggctgcg tactgaacag atggatcagc
     1741 gggctggtgt cctgcacccg attaagccgt gcgacacgag aatcgcgacc ctgcgccgtc
     1801 tcaataatcc cgcgcccgcg cagcacggtc agcccttcac gcagtgcgga gcgtgagaag
     1861 ccgagctttt cacacagtcg acgttccgag ggaagtggct gaccgacctt cagtacgccg
     1921 tcgataatta accgttcgat actctctgca accacttcgc aaatagggcg acgttcatct
     1981 ttcattcccg gacctcgtgc acaggtagga ccaatttttc tgttcttaac tcgcaaaaca
     2041 cgcacatcac gtaagtgatt atgttacatc aatttaacat tgagttaacc aaggcaaggt
     2101 cacagagctg gaaaaaaaat ggtctgaccg gtagctaaag agatagacga aaacgaaaag
     2161 cccgcttaat aactgttcac agaagcagcg cgcaaaaatc agctgccaca caacacaaca
     2221 aagcgaagcc tactcatgag catcttgtac gaagagcgtc ttgatggcgc tttacccgat
     2281 gtcgaccgca catcggtact gatggcactg cgtgagcatg tccctggact tgagatcctg
     2341 cataccgatg aggagatcat tccttacgag tgtgacgggt tgagcgcgta tcgcacgcgt
     2401 ccattactgg ttgttctgcc taagcaaatg gaacaggtga cagcgattct tgctgtctgc
     2461 catcgcctgc gtgtaccggt ggtgacccgt ggtgcaggca ccgggctttc tggtggcgcg
     2521 ctgccgctgg aaaaaggtgt gttgttggtg atggcgcgct ttaaagagat cctcgacatt
     2581 aaccccgttg gtcgccgcgc gcgcgtgcag ccaggcgtgc gtaacctggc gatctcccag
     2641 gccgttgcac cgcataatct ctactacgca ccggaccctt cctcacaaat cgcctgttcc
     2701 attggcggca atgtggcgga aaatgccggc ggcgtccact gcctgaaata tggtctgacc
     2761 gtacataacc tgctgaaaat tgaagtgcaa acgctggacg gcgaggcgct gacgctggga
     2821 tcggacgcgc tggattcacc tggttttgac ctgctggcgc tgttcaccgg atcggaaggt
     2881 atgctcggcg tgaccaccga agtgacggta aaactgctgc cgaagccgcc cgtggcgcgg
     2941 gttctgttag ccagctttga ctcggtagaa aaagccggac ttgcagttgg tgacatcatc
     3001 gccaatggca ttatccccgg cgggctggag atgatggata acctgtcgat ccgcgcggcg
     3061 gaagatttta ttcatgccgg ttatcccgtc gacgccgaag caattttgtt atgcgagctg
     3121 gacggcgtgg agtctgacgt acaggaagac tgcgagcggg ttaacgacat cttgttgaaa
     3181 gcgggcgcga ctgacgtccg tctggcacag gacgaagcag agcgcgtacg tttctgggcc
     3241 ggtcgcaaaa atgcgttccc ggcggtagga cgtatctccc cggattacta ctgcatggat
     3301 ggcaccatcc cgcgtcgcgc cctgcctggc gtactggaag gcattgcccg tttatcgcag
     3361 caatatgatt tacgtgttgc caacgtcttt catgccggag atggcaacat gcacccgtta
     3421 atccttttcg atgccaacga acccggtgaa tttgcccgcg cggaagagct gggtgggaag
     3481 atcctcgaac tctgcgttga agttggcggc agcatcagtg gggaacatgg catcgggcga
     3541 gaaaaaatca atcaaatgtg cgcccagttc aacagcgatg aaatcacgac cttccatgcg
     3601 gtcaaggcgg cgtttgaccc cgatggtttg ctgaaccctg ggaaaaacat tcccacgcta
     3661 caccgctgtg ctgaatttgg tgccatgcat gtgcatcacg gtcatttacc tttccctgaa
     3721 ctggagcgtt tctgatgcta cgcgagtgtg attacagcca ggcgctgctg gagcaggtga
     3781 atcaggcaat tagcgataaa acgccgctgg tgattcaggg cagcaatagc aaagcctttt
     3841 taggtcgccc tgtcaccggg caaacgctgg atgtgcgttg tcatcgcggc attgttaatt
     3901 acgacccgac cgagctggtg ataaccgcgc gtgccggaac gccgctggtg gcgattgaag
     3961 cggcgctgga gagcgccggg caaatgcttc cctgcgagcc gccgcattat ggtgaagaag
     4021 ccacctgggg tgggatggtc gcctgcgggc tggcggggcc gcgtcgcccg tggagcggtt
     4081 cggtccgcga ttttgtcctc ggcacgcgca tcattaccgg cgctggaaaa catctgcgtt
     4141 ttggtggcga agtgatgaaa aacgttgccg gatacgatct ctcacggtta atggccggaa
     4201 gctacggttg tcttggcgtg ctcactgaaa tctcgatgaa agtgttaccg cgaccgcgcg
     4261 cctccctgag cctgcgtcgg gaaatcagcc tgcaagaagc catgaacgaa atcgcccagt
     4321 ggcaactcca gccgctaccc attagtggct tatgttactt cgacaatgcg ttgtggatcc
     4381 gccttgaggg cggcgaagga tcggtaaaag cggcgcgtga actgctgggg ggcgaagagg
     4441 ttgcgggtca gttctggcag caattgcgtg aacaacaact gccgttcttc tcgttaccag
     4501 gtaccttatg gcgcatttca ttacccagcg atgcgccgat gatggattta cccggcgagc
     4561 aactgatcga ctggggcgga gcgttacgct ggctgaaatc gacagccgag gacaatcaaa
     4621 tccatcgcat cgcccgcaac gctggcggtc atgcgacccg ctttagtgcc ggagatggtg
     4681 gctttgcccc gctatcggct cctttattcc gctatcacca gcagcttaaa cagcagctcg
     4741 acccttgcgg cgtgtttaac cccggtcgca tgtacgcgga actttgagga gcaggctatg
     4801 caaacccaat taactgaaga gatgcggcag aacgcgcgcg cgctggaagc cgacagcatc
     4861 ctgcgcgcct gtgttcactg cggattttgt accgcaacct gcccaaccta tcagcttctg
     4921 ggcgatgaac tggacgggcc gcgcgggcgc atctatctga ttaaacaggt gctggaaggc
     4981 aacgaagtca cgcttaaaac acaggagcat ctcgatcgct gcctcacttg ccgcaattgt
     5041 gaaaccacct gtccttctgg tgtgcgctat cacaatttgc tggatatcgg gcgtgatatt
     5101 gtcgagcaga aagtgaaacg cccactgccg gagcgaatgc tgcgcgaagg attgcgccag
     5161 gtagtgccgc gtccggcggt cttccgtgcg ctgacgctgg tagggctggt gctgcgaccg
     5221 tttttaccgg aacaggtcag agcaaaactg cctgctgaaa cggtgaaagc taaaccgcgt
     5281 ccgccgctgc gccataagcg tcgggtttta atgctggaag gctgcgccca gcctacgctt
     5341 tcgcccaaca ccaacgcggc aactgcgcga gtgctggatc gtctggggat cagcgtcatg
     5401 tcagctaacg aagcaggctg ttgtggcgcg gtggactatc atcttaatgc gcaggagaaa
     5461 gggctggcac gggcgcgcaa taatattgat gcctggtggc ccgcgattga agcaggtgcc
     5521 gaggcaattt tgcaaaccgc cagcggttgc ggcgcgtttg tcaaagagta cgggcagatg
     5581 ctgaaaaacg atgcgttata tgccgataaa gcgcgtcaag tcagtgaact ggcggtcgat
     5641 ttagtcgagc ttctgcgcga ggaaccgctg gaaaaactgg cagttcgcgg cgataaaaaa
     5701 ctggccttcc actgcccgtg taccctacaa catgcgcaaa agctgaacgg cgaagtggaa
     5761 aaagtgttgc ttcgtcttgg atttacctta acggacgttc ccgacagcca tctgtgctgc
     5821 ggttcagcgg gaacatatgc gttaacgcat cccgatctgg cacgccagct gcgggataac
     5881 aaaatgaatg cgctggaaag cggcaaaccg gaaatgatcg tcaccgccaa cattggttgc
     5941 cagacgcatc tggcgagcgc cggtcgtacc tctgtgcgtc actggattga aattgtagaa
     6001 caagcccttg aaaaggaata aaaaaatgaa aactaaagtc attcttagcc agcaaatggc
     6061 gagtgcaatt attgccgcag gtcaggaaga ggcgcagaaa aataactggt ctgtttccat
     6121 tgctgttgcc gatgacggcg gtcatctgct ggcgttaagt cgcatggacg attgcgcgcc
     6181 gattgcggct tacatctccc aggagaaagc gcgtaccgcc gcgctggggc gtcgtgaaac
     6241 taagggctat gaagagatgg tgaacaacgg acgtaccgcg ttcgtgactg cgccgttact
     6301 gacgtcgctg gaaggtggcg taccggttgt tgttgatggg caaattattg gtgccgtggg
     6361 cgtttctggt ttaaccggag cacaggatgc gcaggtcgcg aaagcggcag cagcggtgtt
     6421 ggcgaaataa gcgaaaacga ggagataaac aatgagtcaa accataaccc agggccgttt
     6481 acgcattgac gccaatttta aacgttttgt ggatgaagaa gttttaccgg gaacagggct
     6541 ggacgctgcg gcgttctggc gcaattttga tgaaatcgtt cacgatctgg caccagaaaa
     6601 tcgtcagttg ctggcagaac gcgatcgcat tcaggcggcg cttgatgagt ggcatcgcag
     6661 caatccgggg ccggtaaaag ataaagcggc ctataaatct ttcctgcgtg aactgggcta
     6721 cctggtgccg caaccggagc gcgtgacggt ggaaaccacg ggcattgaca gcgaaatcac
     6781 cagtcaggcg gggccacagc tggtggttcc ggcaatgaac gcccgctacg cgctgaacgc
     6841 ggcgaacgct cgctggggct cactgtacga tgcgttatac ggcagcgaca tcatcccgca
     6901 ggaaggggcg atggtcagcg gctacgatcc gcaacgcggt gagcaggtta tcgcctgggt
     6961 tcggcgtttc ctcgatgaat ctctaccgct ggaaaatggc agctatcagg atgtggtggc
     7021 gtttaaggtg gtcgataaac aattacgcat ccagttgaaa aatggtaaag aaaccacgtt
     7081 acgtaccccg gcgcagtttg tcggttaccg tggcgatgcc gctgcgctga cctgcatttt
     7141 gctgaaaaat aacggcctgc atattgaact gcaaattgat gccaacgggc ggattggcaa
     7201 agacgatccg gcgcacatca acgatgttat cgtcgaagcg gctatcagta ccattctcga
     7261 ctgcgaagat tcggtcgcgg cggttgatgc ggaagataaa atcctgctgt accgcaatct
     7321 gctgggcctg atgcagggga ctctgcaaga gaaaatggag aaaaacggtc gacaaatcgt
     7381 gcgtaaactg aatgacgatc gtcattacac cgccgccgat ggctctgaaa tttctctgca
     7441 cggacgctcg ctgctgttta tccgcaacgt gggtcatttg atgaccattc ctgtgatttg
     7501 ggacagcgaa ggcaatgaaa tcccggaagg cattcttgat ggcgtcatga ctggcgcgat
     7561 cgccctctat gatttaaaag tgcagaaaaa ctcgcgcact ggcagcgtct atattgttaa
     7621 accgaaaatg cacggtccgc aggaagtggc gttcgccaac aaactgttta cccgcattga
     7681 gacaatgctc ggtatggcac cgaataccct gaaaatgggc attatggatg aagaacgtcg
     7741 gacctcgctg aacttgcgta gctgtatcgc tcaggcgcgc aatcgcgtgg cgttcatcaa
     7801 taccggtttc ctcgaccgta ccggcgatga aatgcattcg gtgatggaag ctggcccgat
     7861 gctgcgtaaa aaccagatga aatcgacgcc gtggatcaaa gcctacgagc gtaataacgt
     7921 gctttccggt ctgttctgtg ggctgcgcgg taaagcgcaa attggtaaag gcatgtgggc
     7981 aatgccggac ctgatggcag acatgtacag ccagaagggc gaccaactgc gtgccggggc
     8041 aaacacagcc tgggttccgt caccaaccgc tgctacgctc catgcgctgc actaccacca
     8101 aaccaacgta cagagcgtgc aagccaacat tgcccagacc gagttcaatg ctgaatttga
     8161 accgctgctg gacgatctgc tgactattcc ggtggctgaa aacgctaact ggtcggtgga
     8221 agagatccaa caagagctgg ataacaacgt gcaggggatt ctggggtacg tggtgcgctg
     8281 ggtggagcag gggattggtt gttcaaaagt tccggatatt cacaatgtgg cgctgatgga
     8341 agaccgcgca acgctgcgta tctccagcca gcatatcgcc aactggttac gtcacggtat
     8401 tctgaccaaa gaacaggtgc aggcgtcgct ggagaatatg gcgaaagtgg ttgatcagca
     8461 aaacgctgac gatccggctt atcgtccgat ggcggggaat ttcgctaact cgtgtgcttt
     8521 taaagctgcc agcgatttaa tcttcctcgg cgtgaaacag ccaaacggct ataccgaacc
     8581 gttattacac gcctggcgtt tacgcgaaaa agaaagtcat taatagcgtc aaaaaaagcc
     8641 ccgacaagtt ttattgtcgg ggctatgata gtaactgcgg tagtacattt atcccggaaa
     8701 taaccggagc gattatcagg gtttaacaat attaaatacc cgtagcgcat tactagcggg
     8761 cctataacaa caatatggac atcttaacga tactctgcgc ctgcgtgtgc agaacagaaa
     8821 cccttacccg atatacttgt tatgccagag gtagcaattg cgttagcggc gtactgttgc
     8881 tgtaaagtat aacgcctata ttttacagtt cagtcctctt ttgcttatta caaccttaag
     8941 tatatcaagc atataaagat aataagagac tgaacaatat ggttacctgg acccaaatgt
     9001 atatgccgat gggaggactg gggctatccg ctctggtcgc cctgatcccg ataatattct
     9061 tcttcgttgc actcgcggta ttacgtctga aaggacatgt cgctggagca ataaccctta
     9121 tattatctat cctgattgca atattcgcct ttaaaatgcc gattgatatg gcttttgctg
     9181 ctgcgggcta tggctttatt tatggattat ggcctattgc gtggattatt gtcgcggcgg
     9241 tgttcctcta taaattaacc gttgccagcg gtcagttcga tattatccgc agctcggtta
     9301 tctccatcac cgacgatcag cgtttgcagg tgttactgat tggtttctcc tttggtgcgt
     9361 tactggaagg agcggctggc tttggtgcgc cggtggcgat taccggtgcg ctgctggtgg
     9421 gcctgggctt caaaccgtta tacgcggcgg ggctgtgtct gattgccaac actgcgccag
     9481 tggcgtttgg tgcgttgggc gtgccgattc tggtcgccgg tcaggtaacg ggaatcgatc
     9541 cgttccacat tggcgcaatg gcgggacgtc agttaccgtt cctgtcggtt cttgtgccgt
     9601 tctggctggt agcaatgatg gacggctgga aaggggtgaa agagacgtgg ccagcggcgc
     9661 tggttgctgg gggaagcttt gctgtcactc agttctttac ctctaactat attggtccgg
     9721 aactgccgga tattacttcg gcgctggtga gtatcgtctc actcgcttta ttccttaaag
     9781 tctggcggcc gaaaaatacc gaaacggcaa tcagcatggg gcaatccgca ggtgcgatgg
     9841 tggtaaataa gccatcttct ggcggtcccg tgccttcaga atatagtctg gggcaaatca
     9901 ttcgtgcgtg gtcaccgttt ttaatcttaa cggtgctggt caccatctgg accatgaagc
     9961 cgtttaaagc gttatttgct ccgggcggcg cgttttattc actggtgatt aatttccaga
    10021 tccctcattt gcatcaacaa gtgttgaaag cggcacccat tgtcgcccaa ccaacgccaa
    10081 tggatgcggt gtttaaattc gaccctctct cggctggcgg caccgctatt tttattgcgg
    10141 cgattatctc tatcttcatc ctcggcgtgg gcatcaagaa agggattggc gtctttgccg
    10201 aaacgctaat tagtctgaag tggccgatat tgtcgattgg catggtgctg gcgttcgcct
    10261 tcgtcaccaa ctattccggc atgtccacca cgttggcgct ggtactggca ggtacaggcg
    10321 tgatgttccc gtttttctca ccgttcctcg gctggctggg ggtattcctt accggctcgg
    10381 acacttcttc aaacgccctg tttggttcac tgcaatcgac cacggcacag caaatcaacg
    10441 tctctgacac tctgctggtg gcagcaaata ccagcggcgg cgtaactggc aagatgatct
    10501 ccccgcaatc tatcgccgtg gcctgcgccg cgacgggcat ggtgggccga gaatctgaac
    10561 tattccgcta caccgtgaag cacagtctga tttttgccag cgttatcggc gttatcacgt
    10621 tgttgcaggc gtatgtgttt actggaatgt tggtctcgta aatcaataat gccggatgat
    10681 cgttaatatc atccggcttc atttacatta ttctgtctta t
//
LOCUS       DAPHDW010000151        10617 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.153, whole genome shotgun sequence.
ACCESSION   DAPHDW010000151 DAPHDW010000000
VERSION     DAPHDW010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10617
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.153"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..623
                     /locus_tag="RH057_003788"
     CDS             57..623
                     /locus_tag="RH057_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HDV3130092.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALYLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            627..1202
                     /locus_tag="RH057_003789"
     CDS             627..1202
                     /locus_tag="RH057_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HDV3130093.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTAKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(1244..2296)
                     /locus_tag="RH057_003790"
     CDS             complement(1244..2296)
                     /locus_tag="RH057_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HDV3130094.1"
                     /translation="MPVLHNRISNDALKAKMLAESEPRTTISFYKYFHIADPKATRDA
                     LYQLFTALNVFGRVYLAHEGINAQISVPASNVETFRAQLYAFDPALEGLRLNIALDDD
                     GKSFWVLRMKVRDRIVADGIDDPHFDASNVGEYLQAAEVNAMLDDPDVLFIDMRNHYE
                     YEVGHFENALEIPADTFREQLPKAVEMMQAHKDKKIVMYCTGGIRCEKASAWMKHNGF
                     NKVWHIEGGIIEYARKAREQGLPVRFIGKNFVFDERMGERISDEIIAHCHQCGAPCDS
                     HTNCKNDGCHLLFIQCPVCAEKYKGCCSEICCEESALPPEEQRRRRAGRENGNKIFNK
                     SRGRLNTTLGIPDPTE"
     gene            2521..3441
                     /gene="lpxL"
                     /locus_tag="RH057_003791"
     CDS             2521..3441
                     /gene="lpxL"
                     /locus_tag="RH057_003791"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HDV3130095.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPVIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            3613..4839
                     /gene="mdtG"
                     /locus_tag="RH057_003792"
     CDS             3613..4839
                     /gene="mdtG"
                     /locus_tag="RH057_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtG"
                     /protein_id="HDV3130096.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSITFLFSAIASPFWGGLADRKGRKLMLLRSALGMGIVMVLMG
                     LAQNIWQFLILRALLGLLGGFVPNANALIATQVPRNKSGWALGTLSTGGVSGALLGPM
                     AGGLLADSYGLRPVFFITASVLILCFFVTLFCIREKFQPVSKKEMLHMREVVTSLKNP
                     KLVLSLFVTTLIIQVATGSIAPILTLYVRELAGNVSNVAFISGMIASVPGVAALLSAP
                     RLGKLGDRIGPEKILITALIFSVLLLIPMSYVQTPLQLGILRFLLGAADGALLPAVQT
                     LLVYNSSNQIAGRIFSYNQSFRDIGNVTGPLMGAAISANYGFRAVFLVTAGVVLFNAV
                     YSWNSLRRRRIPQVSN"
     gene            4922..5296
                     /locus_tag="RH057_003793"
     CDS             4922..5296
                     /locus_tag="RH057_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HDV3130097.1"
                     /translation="MTMYATLEEAIDAAREEFLADNPGIDAEDANVQQFNAQKYVLQD
                     GDIMWQVEFFADEGEEGECLPMLSGEAAQSVFDGDYDEIEIRQEWQEENTLHEWDEGE
                     FQLEPPLDTEEGRAAADEWDER"
     gene            complement(5297..5524)
                     /locus_tag="RH057_003794"
     CDS             complement(5297..5524)
                     /locus_tag="RH057_003794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HDV3130098.1"
                     /translation="MRLIVVSIMVTLLSGCGSIISRTIPGQGHGNQYYPGVQWDVRDS
                     AWRYVTILDLPFSLVFDTLLLPIDIHHGPYE"
     gene            complement(5697..8240)
                     /gene="mdoH"
                     /locus_tag="RH057_003795"
     CDS             complement(5697..8240)
                     /gene="mdoH"
                     /locus_tag="RH057_003795"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HDV3130099.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
     gene            complement(8233..9768)
                     /gene="mdoG"
                     /locus_tag="RH057_003796"
     CDS             complement(8233..9768)
                     /gene="mdoG"
                     /locus_tag="RH057_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HDV3130100.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPSPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPQGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKTTEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            10162..>10617
                     /locus_tag="RH057_003797"
     CDS             10162..>10617
                     /locus_tag="RH057_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV3130101.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTL"
BASE COUNT         2608 a         2781 c         2612 g         2616 t
ORIGIN      
        1 attcattctc cgacacgcca ttttctttct ctcgcaaatt cgttttggat gcacttatgt
       61 cattcacaaa tacccctgaa cgctatggtg ttatatctgc ggccttccat tggctaagcg
      121 caattattgt ctacggcatg tttgctttag gtctgtggat ggtcacgctc agttattacg
      181 atggctggta tcacaaagca cccgaactgc ataaaagcat cggtattttg ttaatgatgg
      241 ggctggttat tcgcgttctg tggcgtgtca tatctccacc gccgggtccg ctgccgagtt
      301 attcgcccat gactcgtctt gccgcaaaag ctggacatct cgcattgtac ctattgctgt
      361 tcgccatcgg catcagcggc tatctgatct caactgccga tggtaaaccg atcagcgttt
      421 ttggctggtt tgacgtcccc gcgacccttg ccgacgctgg cgcacaggca gactttgccg
      481 gtgccctgca tttttggctc gcgtggagcg tcgtagtact gtccgttatg cacggattta
      541 tggccctgaa gcatcatttc atcgataaag acgacactct gaagcgcatg ctgggaaagt
      601 cgtcatctga ctatggagta taaaaaatga aaaaaagcct gcttggttta accttcgcgt
      661 ccctgatgtt ctctgccggt tcagcggttg ccgccgatta caaaattgac aaagaaggtc
      721 agcacgcctt tgttaatttc cgcatccagc accttggcta tagctggtta tacggcacct
      781 ttaaagattt cgacggtact tttacctttg acgaaaaaaa tccggctgcc gataaagtga
      841 atgtgacaat taacaccacc agcgtcgata ctaatcacgc cgaacgcgat aaacatcttc
      901 gcagtgcaga tttcctcaat accgcaaaat atccacaggc aacattcacc tccaccagcg
      961 tgaagaaaga cggtgacgaa ctggatatta ccggcgatct gacgctgaat ggcgtaacca
     1021 aacccgtcac gctggaagcg aaattaattg gtcagggcga cgacccatgg ggtggtaaac
     1081 gtgcaggctt cgaggccgaa ggcaaaatta agctcaagga cttcaatatc aagacagatt
     1141 taggtccagc ttctcaggaa gtggatctga ttatttcagt ggaaggcgta cagcagaagt
     1201 aagcgtgaaa tgttgccgga tggcacgcat ccggcaatga tatttattct gttggatcag
     1261 gaatgcccag tgttgtattc agacgtccac gagacttatt aaagatctta ttgccatttt
     1321 cacgtcctgc ccgacggcgt cgctgttctt ctggcggtaa cgcgctttct tcgcagcaaa
     1381 tctcactaca acaacctttg tatttttccg cgcatactgg acactgaata aacagcaggt
     1441 ggcagccatc atttttacag ttggtatggc tgtcgcacgg cgcgccgcac tggtggcaat
     1501 gcgcgataat ctcatccgat atacgttcgc ccatccgctc gtcaaaaaca aaatttttgc
     1561 caataaaacg caccggcaag ccctgctcgc gcgccttacg ggcgtattca ataattccgc
     1621 cctcgatatg ccagactttg ttgaatccgt tatgtttcat ccaggcactg gccttttcac
     1681 aacgaatgcc gccggtgcag tacatgacga tttttttatc tttatgtgcc tgcatcatct
     1741 cgactgcttt tggcagctgc tcacggaagg tatctgccgg aatttcgagc gcgttttcaa
     1801 agtgccccac ttcatactca tagtggttac gcatgtcgat aaatagtaca tcgggatcgt
     1861 caagcatggc gttcacttcc gccgcttgca gatactcacc aacattgctg gcatcaaagt
     1921 gaggatcgtc aataccgtcg gcaacaatgc gatcgcgtac cttcatgcgc agtacccaga
     1981 aggatttccc gtcatcatcc aacgcgatat tcaggcgtaa accctctaaa gccgggtcga
     2041 aggcatagag ctgcgcgcga aatgtttcaa cattgctcgc aggtacgctg atttgcgcgt
     2101 taatgccctc atgcgccaga tacactcgcc caaaaacatt cagcgcggta aacagctgat
     2161 ataaagcgtc acgggtcgcc ttaggatcgg cgatgtggaa atacttgtaa aacgaaatgg
     2221 tggttcgcgg ttcgctctca gccaacattt tggcttttag cgcgtcgttg gaaatgcggt
     2281 tgtgtaacac tggcatggtg tacggttcct gcgagatggg aaagtaaaaa tccgcggcat
     2341 gatatagcaa ttatcgataa ttaacatcca cacattttac gctacatttg cgcattaaaa
     2401 attatttgtt atttacaagc gcggcaattt cgcccagtct tcagccacaa attttggttg
     2461 cgggcgaaaa aatgcgacaa tacatacaat tgcccgaata ggttgaaaaa caggattgat
     2521 atgacgaatc tacccaagtt ctccaccgca ctgcttcatc cgcgttattg gttaacctgg
     2581 ttgggtattg gcgtactttg gttagtcgtg caattgccct acccggttat ctaccgcctc
     2641 ggttgtggat taggaaaact ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat
     2701 cgcaacctgg aactgtgctt cccggaaatg agcgaacaag aacgccgtaa aatggtggtg
     2761 aagaatttcg aatccgttgg catgggcctg atggaaaccg gcatggcgtg gttctggccg
     2821 gaccgccgaa tcgcccgctg gacggaagtg atcggcatgg aacacattcg tgacgtgcag
     2881 gcgcaaaaac gcggcatcct gttagttggc atccattttc tgacactgga gctgggtgcg
     2941 cggcagtttg gtatgcagga accggggatt ggcgtttacc gcccgaacga taatccactg
     3001 attgactggc tacaaacctg gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa
     3061 gatttaaaag gcatgattaa agccctgaaa aaaggcgaag tggtctggta cgcaccggat
     3121 catgattacg gcccgcgctc aagcgttttc gtcccgttat ttgccgttga gcaggctgcg
     3181 accacgaccg gaacctggat gctggcacgg atgtccggcg catgtctggt gcccttcgtt
     3241 ccacgccgta agccagatgg caaagggtat caattgatta tgctgccgcc agagtgttct
     3301 ccgccactgg atgatgccga aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc
     3361 atcatgatgg caccagagca gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa
     3421 ggcgttcctt cacgctatta aatctcccct gccggatgct tcagaatggc atccggcatt
     3481 accacagcaa atccccctga tttagcgata aaagctctct ggattgcgcc ccctggaagt
     3541 cgggcgcata attagtgtgc ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa
     3601 cagcggattg ctatgtcacc ctgtgaaaat gacaccccta taaactggaa acgaaacctg
     3661 atcgtcgcct ggctaggctg ttttcttacc ggtgccgcct tcagtctggt aatgcccttc
     3721 ctacccctct acgttgagca gcttggagtt accggtcact ccgccctgaa tatgtggtcc
     3781 ggtattgtct tcagcattac atttttattt tcggccatcg cctcaccgtt ttggggtgga
     3841 ctcgccgacc gtaaaggccg aaaactcatg ctattacgct ctgccctcgg catgggcatc
     3901 gtgatggtgt tgatgggact ggcacaaaat atctggcagt ttttgatcct acgggcgctt
     3961 cttgggttac ttggcggatt tgtccccaac gctaatgctc ttatcgccac acaagtaccg
     4021 cgtaataaaa gcggctgggc gctgggtacg ctctccacag gcggcgttag tggtgcgttg
     4081 ctcggcccaa tggctggcgg cctgctcgcc gatagctacg gcttacgtcc ggtattcttt
     4141 attaccgcca gtgtgctcat actctgcttt ttcgtcaccc tgttctgcat cagagaaaaa
     4201 ttccagccgg tcagcaaaaa agagatgctg cacatgcggg aagtggtgac atcacttaaa
     4261 aacccgaaac tggtactcag cctgtttgtc accacgttaa tcatccaggt ggcgacaggc
     4321 tcaattgccc ccattctgac gctgtatgtc cgcgaactgg cgggtaacgt cagtaacgtc
     4381 gcctttatca gtggcatgat cgcctcggtg ccaggcgtgg cggctctgct aagcgcacca
     4441 cgactcggca aacttggcga tcgaatcgga cccgaaaaga tcctgattac agcgctgatc
     4501 ttttctgtac tgctgttgat cccaatgtct tacgttcaga cgccattgca acttgggatt
     4561 ttacgttttt tgctcggtgc cgccgatggt gcactactcc ccgccgtaca gacactgttg
     4621 gtttacaact cgagcaacca gatcgccggg cgtatcttca gctataacca atcgtttcgt
     4681 gatattggca acgttaccgg accattgatg ggagcagcga tttcagcgaa ctacggtttc
     4741 agagcggtat ttctcgtcac cgctggcgta gtgttattca acgcagtcta ttcatggaac
     4801 agtctacgtc gtcgtcgaat accccaggta tcgaactgat ttttcgcctt tcatacttgc
     4861 aaaagcggag aatcagctat ccttttccct gaaacctcat caactcaaag ggagaatcgt
     4921 gatgaccatg tacgcaacgc ttgaagaagc cattgacgct gcacgcgaag aatttcttgc
     4981 agacaacccc ggcatcgacg ccgaagatgc gaatgtgcaa cagttcaatg cccaaaaata
     5041 cgttttgcag gacggcgaca tcatgtggca agttgagttt tttgccgacg aaggggaaga
     5101 aggtgaatgt ttacctatgc ttagcggtga agccgcgcaa agtgtttttg atggcgacta
     5161 tgatgagata gagatacgcc aggagtggca ggaagagaat acattacatg aatgggacga
     5221 gggggaattt cagcttgagc caccgctgga taccgaggaa ggacgcgcag cagctgatga
     5281 gtgggatgaa cgttaatcac tcatacgggc catgatgaat gtcgatcggc agcagtaaag
     5341 tatcaaaaac cagagagaat ggcagatcaa ggatcgtgac ataacgccag gcggagtcac
     5401 gcacatccca ttgcacaccg ggataatatt ggttgccatg cccctgcccc ggtatagtgc
     5461 gactaataat gctgccacag ccactcagca aggtcaccat gatgctcacc acaattaaac
     5521 gcatttgata cctctttcgt aaaaaaatac cggcataacg ccggtatttt tattggctcg
     5581 ttgtttttgc cggatgcggc gtgaacgcct tatccgaccc acaaaatctt gcaaaatcaa
     5641 taaattgcag gaacgatgta ggcctgataa gcgtagcgca tcaggcaact acgtttttat
     5701 tgcgaagccg catccggttt acgcaatgcc agtggattga gctttatccc ttcgtaataa
     5761 ctcacccatg aagaatatct ctccggggaa ttccagacgc ggaaatgcag acgggccatc
     5821 gtcaccggat cgcttagcag caccaggcga cgatcgcgat tcagcttctc tggcgtctcg
     5881 ttcagcgcct gttcaacgtg gcggtcacgg gcgatttcca gcaccttact ggcgcggtga
     5941 cgcgcggtcg ccattgcggt tgccagagcg ttaaatgacg ggttaaacac tgcgtgcata
     6001 aagccatcat caagggagcg ttgacgattc atctcaagga accgatcggt atcaaccagc
     6061 acctgcggcg gcgaatactc ttccgggatc aggaacagtt tccagcgttt ggtgcgcaga
     6121 ccaacggtgg cacggctgga aatcaccgaa acaaacggtg acaggatcaa cgagaagaca
     6181 atcggtgcca gccagaacag gaaacgcaga tccagccacg ccatcccaac agcccacact
     6241 aaccctaaca gcagctgtga gccgtggcgt ttgaacgctt caccccagga agtggagtca
     6301 tcatcacgct gcggtgaatt ccacaccact tcccagccaa ggaacgcgct gacaacgaag
     6361 accgtatgga acagcatgcg taccggagcc agcagcacgg aaaaaagcac ttccagcagc
     6421 aacgataatg taacgcgcca gaagccgccg tattctttcg ttcctttgca ccagataagc
     6481 aaaatgctca ataacttcgg caggaacaac agcaccatag tcgaagcaaa aagtgcaatc
     6541 gccagctcag gacgccactg cggccacacc gggaacaact gccgtggttg caggaagtat
     6601 tgcggttcgg tcaacgcatg tactacctgc aatgcagtag agagcgcgag gaacataaac
     6661 cacagcggag cggagagata agacatcacg cccgtcagga acaccgcacg gtgaaccggg
     6721 tgcataccct taaccaggaa cagacggaag ttcatcaggt taccgtggca ccagcggcgg
     6781 tcacgtttta gctcatcaag caagttaggc ggtaattctt cataagaacc cgggagatcg
     6841 taagcaatcc agacccccca acctgcacgg cgcatcaacg ccgcttccac gaagtcatgt
     6901 gacaggattg aaccggcaaa ggaaccttcg cccggcagcg gagccagtgc gcagtgctcg
     6961 ataaacggtt tcacgcggat aatcgcgtta tgtccccagt agtgcgactc gccaagttgc
     7021 cagaagtgca aaccggctgt aaacagtggc ccatacacgc gggtcgcgaa ctgctgacag
     7081 cgcgcataca gcgtatccat accggacgct ttcggcgacg actgaatgat cccggcgttc
     7141 gggttggctt ccatcaggcg caccagcccg cacaaacaat caccggtcat taccgagtca
     7201 gcatccagca ccaccatgta gctgtactgg ctgccccagc gacggcagaa gtcatcgata
     7261 ttaccgcttt tacgcttcac gcggcgacgg cggcggcgat agaaaatctg accttcgcca
     7321 ccgacttcag cgataagctc catccaggct ttttgctctg cgacgcagat atccgggtta
     7381 taactgtcac taagaatgta gacatcaaag tgtttggcat tcccggtggc ttttactgat
     7441 tcccacgttg cacgcaggcc agcaaaaaca cggttcacgt cttcgttaca gataggcatg
     7501 atcaacgccg tgcgatgctc cgggtttaat ggttcatcgc caactgttga cgcagatata
     7561 ctgtatttat cgcgaccaat aagcagttgc aggaagccca ttaacgccgt ccagaatccg
     7621 gcggacaccc aacagaacag taccgcaaag aggatcagga taccggtttg cagcatataa
     7681 ggcagaagct gcataaagga aacccacaaa tcctgaccaa ccatatccat aggattaatc
     7741 agcgcccaac cctgataagg aagaatggtc ttcatatacc aggtcgcgac gacagtttgc
     7801 gcgagcgtca ggatcaacag aatgtaacgg cggatggtac cgacggtacg ccacttttgc
     7861 tcgctctcct gctcttcttt ggtcaaacga gccagatagc gcggcgtgac atcgcgtcca
     7921 cgcaggcgat cccagaaacg gcctaccggg ttggtacgcc acgggtcggg aaacatcgag
     7981 gagcgttttg cttctggcat cgccttcagc tgatcgcgcc cttcgtcgtc tttaattaac
     8041 tgtccatcag caagtgaatc tggccaggct tgttccagac gcgcctttac cgagccttgc
     8101 ggggaatcat cctcccgcgc ccaggtgcgg tgttcggcat ccagcgcctg atgaacggcg
     8161 cggatatcag tcttcggcaa tgccgctttc tcgcttgcgg cgatgggcat tgcgtcaatg
     8221 tactcagttg tcttattcat tggcaggtaa ctggtagctc caggtttcac tcaacgtctg
     8281 atcggcattc accagcgcag cacgcatttc agtggttttc ttggcatctt tcactttcac
     8341 acgcatcacc agacgccagc ctttggtaac cgggttatag cgcaccgtgc tttcaactat
     8401 ctcaccatta tcaccaatgc tggtttgcgc tgtgaccggg gtatcctctg gcagtttttt
     8461 catctctgcg ccggtaaaat cgaccacaaa ggcgatagta ccgtcaggct ggcgaatcag
     8521 gttcgactgc ttcacatccc ccgttgaacg acgcgtttgt tgcacccatg cgttatctgg
     8581 cgcatgcagt ttgtcttcat cacggctgaa ggtgatggtg tatttaaagt tcatctcttt
     8641 acccggctcc ggcagctgat ccggcgtcca gtaagcgacg atgttatcgt tggtttcatc
     8701 gttggttgga atttctacca gctcaacgct gcctttaccc cactcccctt tcggagtcac
     8761 ccatgcgctt ggacgaagat cgtaacgatc atcgagatct tcaaagcggg agaaatcacg
     8821 accgcgctgc aacagaccga agccttgcgg gttttccata gagaagctgc tgaccgctaa
     8881 atgtttcggg ttattcaacg gacgccagat ccattcgcca ttaccggcat ggatagagag
     8941 accgttagag tcgtgcaact ccggacgata gttatttgca ggcgacggtt ggttcggccc
     9001 aaacaggaac atactggtta acggtgcaac ccccagtttg ccgactttat cgcgcagata
     9061 gattttcgac tgcacatcca caaccgtgtc acgccctggc ataaccacga atttgtaagc
     9121 acctgtcgcg cgcggcgagt caagcaatgc ataaatggtt aaacgtttat cagtcggttt
     9181 tggacgctcg atccagaact ctttgaagcg tggaaattct tcacccgatg gcaaggcggt
     9241 atcaattgcc aggccgcggg cagaaaggcc ataaacctga cctgcaccaa tcacgcggaa
     9301 atagctggcc ccgagcatgc tgacgatttc atcgttttta tctttgctgt tgatcgggta
     9361 aagcacttta aaaccggcaa aaccaaggtc ttttaccgtg tctttgtcat gctgaacatc
     9421 gccgaaagtg aaataatccg ggctgtattt gattcgtttg actgcggtgg cagtcacttc
     9481 atttattttg accggggtat cgaagtacat accctgatgg tagaactcga gtttgaatgg
     9541 ggtcttcaga ttgttccagt acgctttgtc atgattaaac tggatctgct gatagtccgc
     9601 gtatttcata tcgcggaaaa cggagggcaa gttgcttttg ggcgcctcat agcctttccc
     9661 ggctaaggat tgagcttgct ttgcgacatc atcaatactg aaagcccagc ttgaagatgt
     9721 atacagggtt aacattactg cagcactcaa ccaacgcatt ttcatcattt gtagtttatg
     9781 tttcataatt agtaagcact tccccctttg tgtgcttata tcgatccgat ctattttaat
     9841 ggataaccag ccattccgac aacagaatcc cggtatcgtt cagcacgctg gctcatggat
     9901 taagcacctg attgcaaccg ttttcacttt ccgtgcttct cctaaagaca gaagcatcga
     9961 cttcgtgtag ggtttgccag caattttatt tatcaaccgt cttacagacg cttccattct
    10021 ggttagtggt agcgatgatg ctatgcataa aaaccttgaa gcctgcgcct ggcgcggtca
    10081 ggtcggtgtc gctatggcaa caagcctaaa ggcataaccc gacagggata agacgaaaaa
    10141 tccgagatta gttaaccata tatgaatcca gtacccgcgc aacgtgaata tttcctcgac
    10201 tccatccgcg cctggctgat gttgttaggg atcccttttc atatttcttt aatctattcg
    10261 agccatacat ggcatgtgaa tagcgccgaa ccatcattgt ggctgaccct ttttaatgac
    10321 ttcatccact cgttccgcat gcaggtattt ttcgttatat ccggctactt ttcctacatg
    10381 ctttttttac gctatccctt gaaaaaatgg tggaaagtac gtgtcgaacg tgtaggtatc
    10441 ccgatgttaa cagccatccc cctactgaca ttaccgcaat ttattatgct gcaatatgtc
    10501 aaaggaaaag cggaaagttg gcctgggctg tcattgtatg acaaatataa tacgttggcc
    10561 tgggaattaa tatcacacct gtggttttta ctggtgttag tagtcatgac gacgctg
//
LOCUS       DAPHDW010000152        10511 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.155, whole genome shotgun sequence.
ACCESSION   DAPHDW010000152 DAPHDW010000000
VERSION     DAPHDW010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.155"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            152..727
                     /gene="ssuE"
                     /locus_tag="RH057_003798"
     CDS             152..727
                     /gene="ssuE"
                     /locus_tag="RH057_003798"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HDV3130102.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQVIDYHHRPQFTPNLQTR
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            720..1679
                     /gene="ssuA"
                     /locus_tag="RH057_003799"
     CDS             720..1679
                     /gene="ssuA"
                     /locus_tag="RH057_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HDV3130103.1"
                     /translation="MRNIIKLALVGLLSVSTIAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1676..2821
                     /gene="ssuD"
                     /locus_tag="RH057_003800"
     CDS             1676..2821
                     /gene="ssuD"
                     /locus_tag="RH057_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMNH2-dependent alkanesulfonate monooxygenase"
                     /protein_id="HDV3130104.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLGETVNFNGKHIHVRGA
                     KLLFPPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKIEQVRAKAAAH
                     GRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTDSVGQQRMAAL
                     HNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGIDSFVLSGYPH
                     LEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
     gene            2832..3623
                     /gene="ssuC"
                     /locus_tag="RH057_003801"
     CDS             2832..3623
                     /gene="ssuC"
                     /locus_tag="RH057_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415454.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter permease
                     SsuC"
                     /protein_id="HDV3130105.1"
                     /translation="MATPVKKWLLRVAPWFLPVGIVAVWQLASSVGWLSTRILPSPEG
                     VVTAFWTLSASGELWQHLAISSWRALIGFSIGGSLGLILGLISGLSRWGERLLDTSIQ
                     MLRNVPHLALIPLVILWFGIDESAKIFLVALGTLFPIYINTWHGIRNIDRGLVEMARS
                     YGLSGIPLFIHVILPGALPSIMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREF
                     LQTDVVVVAIILYALLGKLADVSAQLLERLWLRWNPAYHLKEATV"
     gene            3620..4387
                     /gene="ssuB"
                     /locus_tag="RH057_003802"
     CDS             3620..4387
                     /gene="ssuB"
                     /locus_tag="RH057_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HDV3130106.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLRLLAGLETPTAGDVLAGTTPLAEIQEDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAVGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQEHGFTVLLVTHDVSEAVAMADRVLLIEEGKIGLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
     gene            complement(4594..7206)
                     /gene="pepN"
                     /locus_tag="RH057_003803"
     CDS             complement(4594..7206)
                     /gene="pepN"
                     /locus_tag="RH057_003803"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HDV3130107.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEGALVISNLPERFTLKIINEISPAA
                     NTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNGN
                     RVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDRG
                     NLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKYV
                     LARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSDL
                     GSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMIH
                     TLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPIVTV
                     KDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVNS
                     VLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNDFSRWD
                     AAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVNE
                     MAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLRN
                     ACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKWH
                     QDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAFH
                     AEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLEN
                     LSGDLYEKITKALA"
     gene            7472..8674
                     /gene="pncB"
                     /locus_tag="RH057_003804"
     CDS             7472..8674
                     /gene="pncB"
                     /locus_tag="RH057_003804"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HDV3130108.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            8843..10243
                     /gene="asnS"
                     /locus_tag="RH057_003805"
     CDS             8843..10243
                     /gene="asnS"
                     /locus_tag="RH057_003805"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HDV3130109.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVTGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
BASE COUNT         2386 a         2708 c         2816 g         2601 t
ORIGIN      
        1 aatatgcgtg atatataaag ccctttcttt agtttatttt cagaaaaaga tacacaaaat
       61 ttatatttgg cattttcttg tctctccgac agactgaccg aattgttatc aatgttaaca
      121 aaaaaagaac aattggttat aaggagagag tatgcgtgtc atcaccctgg cgggtagtcc
      181 tcgctttcct tctcgctcca gctccttgct ggaatatgcg cgggaaaaac taaatggcct
      241 cgatgtagag gtttatcact ggaatctgca aaacttcgcc ccggaagatc tgctttatgc
      301 tcgtttcgat agtccggcac tcaagacctt caccgaacag ctgcaacagg ctgatggact
      361 gatagtcgct acacctgtgt ataaagccgc ctattccggc gcgctgaaaa ccctgctcga
      421 cctgctgcca gagcgcgctt tgcaaggcaa agtggtgcta ccgctggcga cgggcggtac
      481 cgtggcccat ctgctggcgg tcgattatgc ccttaaacca gttttaagcg cactgaaagc
      541 tcaggagatc ctgcacggcg tgtttgccga tgactcacaa gtaattgatt accatcacag
      601 accccagttc acgccaaatc tgcaaacccg tcttgatacc gcgctagaaa ctttctggca
      661 ggcattgcac cgccgcgatg ttcaggttcc tgaccttctg tctctgcgag gtaatgccca
      721 tgcgtaacat cattaaactg gcgctggtgg gattgcttag cgtctctacg attgcggttg
      781 ctgcagaatc ctcgcctgaa gcgttacgta taggctatca gaaaggcagt attggtatgg
      841 tactggcaaa aagccaccag ttactggaaa aacgctatcc gcaatcaaaa atctcctggg
      901 tggagttccc cgcgggtccg caaatgttgg aagcgttaaa cgttggcagt attgatctcg
      961 gcagtaccgg ggatattccg ccaatctttg cccaggctgc cggggctgat ttggtatatg
     1021 tgggcgtcga gccaccgaag cccaaagccg aagtgattct ggtggcagaa aacagcccga
     1081 tcaaaaccgt agccgatctt aaaggtcaca aagttgcctt tcagaaaggt tccagttcac
     1141 acaacctttt actgcgtgca ctgcgtcagg ccggacttaa gtttaccgat atccaaccca
     1201 cttacctgac gcccgctgat gcccgcgccg cgttccagca aggtaacgtt gacgcctggg
     1261 ctatctggga tccctactac tctgctgcat tattacaggg cggcgtgcgg gtgctgaaag
     1321 acggcaccga tctcaatcaa actggatcgt tttatctggc agctcgtccc tatgcagaaa
     1381 aaaacggcgc ttttattcag ggcgtactcg caacctttag tgaggccgat gcgttaaccc
     1441 gcagccagcg cgagcaaagc atcgctttac tggcaaaaac gatgggctta ccggcaccgg
     1501 tgattgcctc ttacttagat catcgccctc ctaccaccat caaaccggtt aacgccgagg
     1561 ttgccgcctt acagcagcaa acggcagatc tgttttatga aaatcgtctg gtgccgaaaa
     1621 aagtcgatat tcgccagcgc atctggcagc ccactcaact ggaaggaaaa caattatgag
     1681 tctgaatatg ttctggtttt taccgaccca tggtgacggg cattatcttg gaacggaaga
     1741 aggttcacgc ccggttgatc acggctatct gcaacaaatt gcgcaagcgg cggatcgtct
     1801 gggctatacc ggtgtgctga tcccaacggg gcgatcgtgt gaagatgcgt ggctggtggc
     1861 ggcgtcgatg atcccggtga cgcagcggct gaagtttctt gtcgccctgc gccccagcgt
     1921 aacctcacct accgttgccg ctcgccaggc cgccacgctt gaccgtctct caaatggtcg
     1981 tgcgttgttt aatctggtca caggcagcga tccacaagag ctggcaggcg acggcgtgtt
     2041 ccttgatcat agcgagcgct acgaagcttc ggcggaattt acccaggtct ggcggcgctt
     2101 attgctgggg gaaaccgtca atttcaacgg taaacatatt catgtgcgcg gagcaaaact
     2161 gctcttcccg ccgattcaac agccttatcc tccgctttac tttggcggat cgtcagacgt
     2221 cgcccaggag ctggcggcag aacaagttga tctctacctc acctggggcg aaccgccaga
     2281 actagtaaaa gagaagatcg aacaagtgcg ggcgaaagct gccgcgcatg gacgcaaaat
     2341 tcgtttcggt attcgtctgc atgtgattgt tcgtgaaact aacgacgaag cgtggcaggc
     2401 cgccgagcgg ttaatctcgc atcttgatga tgaaactatc gccaaagcac aggccgcatt
     2461 cgcccggacg gattccgtag gacaacagcg aatggcggcg ttacataacg gcaagcgcga
     2521 caatctggag atcagcccca atttatgggc gggcgttggc ttagtgcgcg gcggtgccgg
     2581 aacggcgctg gtgggcgatg gtcctacggt cgctgcgcga atcaacgaat acgccgcgct
     2641 tggcatcgac agctttgtgc tttcgggcta tccgcatctg gaagaagcgt atcgggtcgg
     2701 cgagttgctg ttcccacatc tggatgtcgc cattccggaa attccccagc cgcagccgct
     2761 gaatccgcaa ggcgaagcgg tggcgaatga ttttatcccc cgtaaagtcg cgcaaagcta
     2821 aggaagaaat aatggcaacg ccagtgaaga agtggttatt gcgcgttgcc ccctggtttt
     2881 taccggtggg catcgtggcg gtgtggcaac tggcctcctc agttggctgg ctgtcgacgc
     2941 gtattttgcc ctcaccggaa ggggtggtga cggcgttctg gacgctctcc gccagcggcg
     3001 aactgtggca gcatctggcg atcagttcct ggcgggcgct gattggcttt tcaattggcg
     3061 gatcgctggg gttgattttg ggactgatta gcgggctatc tcgctgggga gaacggctgc
     3121 tggatacctc aattcagatg ttgcgcaacg taccgcatct ggcgctgatc ccgctggtga
     3181 ttttgtggtt tggcattgat gaatccgcaa aaatctttct ggtggcgctc ggcacgctat
     3241 tccccattta tatcaacacc tggcatggca tccgtaatat cgatcgcggt ctggtggaaa
     3301 tggcgcgtag ctatggatta tccggcatac cgctgtttat ccatgtgatc ctgcctggtg
     3361 ccctgccctc aattatggtc ggcgtgcgtt ttgcgttggg cctgatgtgg ctgacgctga
     3421 ttgttgccga aaccatttct gccaattcag gcattggtta tctggcgatg aatgcgcggg
     3481 agtttttgca aacggacgtg gtggtagtcg ctattattct ttacgccctg ctcggcaaac
     3541 ttgctgacgt cagcgcgcag ctgttagagc gcctctggtt gcgctggaac ccggcttatc
     3601 atttgaagga ggccacggta tgaatactgc tcgtctgaac cagggcacac cattgttgct
     3661 caatgcagta agcaaacatt acgcggaaaa tatcgtcctg aaccaactgg atttacatat
     3721 tccggcaggt cagtttgtgg cggtggtggg ccgcagcggt ggtggcaaaa gtaccctgct
     3781 gcgcctgctg gccgggctgg aaacgccaac cgcaggcgat gtgttggcag gcaccacacc
     3841 gctggctgaa attcaggaag acacgcgaat gatgtttcag gatgcgcgtc tgctaccgtg
     3901 gaaatcggtg attgataacg ttgggttagg ccttaaaggc cagtggcgcg atgccgcgcg
     3961 tcgggcgttg gctgcggtag gactggagaa tcgcgccggg gaatggcctg ccgcactttc
     4021 aggcgggcag aaacagcgag tggcgctggc aagagcgttg attcatcgac cgggtttatt
     4081 gttgcttgat gaaccgctcg gggcactgga cgccttaacg cgactcgaga tgcaggattt
     4141 gattgtgtca ctttggcagg agcacggctt taccgtactg ttggtgacgc atgatgtgag
     4201 cgaagcggta gcaatggctg accgggtgtt gttaattgaa gagggaaaaa ttggtctgga
     4261 tttgacagtg gatatccccc gtccgcgtcg attagggtcg gtgcgattag cggagctgga
     4321 agcggaagta ttgcaacggg taatgcggcg tgggcattct gaacaactga ttcggcgtca
     4381 tggttaatat gccggatgtg gcgcacaaaa ccagcatttt caacggacta gtaggcctga
     4441 taagacacgt caagcatcgc atcaggcagt tgggcacggc tgccggatgc ggcgtgaacg
     4501 ccttatccag cctacaaaac cagcaatttc aacggagttg taggcctgat aagacgcgtc
     4561 aagcgtcgca tcaggcattt tggctcaaaa gtatcaagcc agtgctttgg tgatcttctc
     4621 gtacagatcg ccagagagat tttccagccc tttcaactgt tccagcgccg cgcgcatttt
     4681 ctcctgacgt ttggcatcat aacgtttcag gcgaatcagc ggttcaatca gacgtgaagc
     4741 tacctgcggg ttacggctgt tgaggtcggt aagcatttcc accaggaact ggtaaccgct
     4801 gccatcttcg gcatggaacg ctgccgggtt gctgcccgca aacgcaccaa tcaacgaacg
     4861 aatacggttc gggttgctca tggtaaatga gcgatgctgc aacagaccac gcaccgtctc
     4921 cagcacattc gccgccgggc tggtggcttg caggataaac catttatcca tcaccagacc
     4981 gtcctgatgc cacttatcgt cgtactcctg catcagcgcg tcacggcaag gcagctgtgc
     5041 agcaaccgcc gcagaaagcg ctgccagcgc atcggtcata ttgtttgctt catggtattg
     5101 cttgctcacc agcacgtcag ccagatgcgt ttcaccaaaa gcaaggaagc gcaggcaggc
     5161 attacgcaga gtgcgttttg caatatcttc atgctcaaca cggtactcgc tctggtaatt
     5221 cgcgttgtaa atagccagca gctcatccgc cagttcagtc gccagagtac gagtgagtgc
     5281 ttcgcgtact tcggcaatag caatcgggtc gatgatatcg aacagttcag ccatttcatt
     5341 gacagaaggc agcgtcagga tttctgccgc cagcgccgga tcaatcttct catcgagcag
     5401 caccgcgcgg aaagcgtcag ccacatgtac cggcagagac agcggctgcc cttgctgatg
     5461 acgggcgacg ttcagcttga tgtaggttgc cagcaaactt tgcgccgcat cccaacggga
     5521 gaaatcatta cgcgcatgac gcatcaggaa ggtcagttgc tgatcgctcc acttatattc
     5581 cagtttcact ggcgcagaga attcgcacag cagcgcaggc accggctgga agtagacatt
     5641 atcaaaaaca aaggtctgtt ccgcctgggt gacgttcagc acggaattca ccggatgacc
     5701 gcctttctgc aacgggatca ctttgccttc gttgtcatac aattcgatgg caaatggaat
     5761 atgcagcggc tgtttttctg cctgatccgg cgtggctggc gtgcgctggc tgatggtcag
     5821 ggtgtactgc tcggtttccg gattgtagtc gtctttgacg gtcacaatcg gcgtgccgga
     5881 ctggctgtac caacggcgga aatgggagag atcgacattc gacgcatctt ccatcgcctg
     5941 cacaaagtcg tcgcaggttg ccgcgctgcc atcatggcgc tcgaaataga gttgcatccc
     6001 tttctggaag ttttcttcgc ccagcagggt gtggatcatg cgaatcactt ccgcgccctt
     6061 ctcgtaaacg gtcagggtgt agaagttgtt catctcaatg accatatccg ggcggatcgg
     6121 gtgcgccatc gggctggcgt cttccgcaaa ctgcaatccg cgcatggtgc gcacattatt
     6181 gatacggttt accgcgcggg aaccaaggtc agagctgaat tcctgatcgc ggaagacggt
     6241 taaaccttct ttcaggctga gctggaacca gtcgcggcag gtcactcggt taccggtcca
     6301 gttatggaaa tattcatggc cgataacgcg ttcaatatcg agataatctt tgtcggtggc
     6361 ggtgtcggtg cgggccaaca catatttgga gttaaagata ttcagaccct tattctccat
     6421 tgcgcccata ttgaagaaat ccaccgcgac gatcatatag atgtcgaggt catactccag
     6481 gccaaagcgt tcttcatccc atttcatgga gtttttcagc gaggtcatcg cccacggcgc
     6541 gcgatcaagg ttgccgcgat cgacgtacag ctccagtgct acttcgcgac cagaacgcgt
     6601 ggtaaaggta tcgcgcagta catcaaagtc gcctgccacc agcgcaaaca ggtagcacgg
     6661 tttcgggaac gggtcctgcc actgtaccca atggcgtccg ttttcgagtt cgccttgcgc
     6721 aacgcggtta ccattggaaa gcaggaaggg atatttgatt ttatcggcaa taattttggt
     6781 ggtaaaacgc gccagcacgt ccgggcggtc gagataatac gtaatatggc ggaaaccttc
     6841 ggcttcacac tgggtgcaaa gcgcatcgcc tgactgataa agcccttcca gcgccgtatt
     6901 cgccgccggg cttatttcat taatgatctt aagcgtaaaa cgctccggca aattactgat
     6961 gaccagtgcg ccctcttctt ctttccaggc ggtccacggc tcatcattaa tatgaacaga
     7021 aaccagtttg aggtcttcgc cgttgagacg aagcggagca tctgatgcac catgacggac
     7081 agcctggctg accgcggtaa cgaccgtctt ttgcgcgtcg aggtcaaagg tcaagtcaat
     7141 atcagtaatc tggtaatccg gcgcacgata atcgtgacgg tatttggctt gtggctgttg
     7201 agtcataaat aacctttagc atcttttata gagtctggtg ttcagtctat tcctgttgcg
     7261 taaatcgcgc tatgcagaat cttcatcttt tcaggtacaa acgcctttat tgctacattt
     7321 ttataacata caccgcgtaa tgccatcgac cagaaaggtg gcatatggtg tgatcggggt
     7381 tcaataaatt gcgaaacaag gtatactcca gcagttcctg aagatgttta ttgtactaaa
     7441 cgctcctgta cgaggacgct actgcgcacc tatgacacaa ttcgcttctc ctgttctgca
     7501 ctcgttgctg gatacagatg cttataagtt gcatatgcag caagccgtgt ttcatcacta
     7561 ttacgatgtg catgtcgcgg cggagtttcg ttgccgaggt gacgatctgc tgggtattta
     7621 tgccgatgct attcgtgaac agattcaggc gatgcagcac ctgcgcctgc aggatgatga
     7681 atatcagtgg ctctctgctc tgcctttctt taaggccgac tatcttaact ggttacgcga
     7741 gttccgcttt aacccggaac aagtcaccgt atccaacgat aatggcaagc tggatattcg
     7801 tttaagcggc ccgtggcgtg aagtcatcct ctgggaagtt cctttgctgg cggttatcag
     7861 tgaaatggta catcgctatc gctcaccgca ggccgacgtt gcgcaagccc tcgacacgct
     7921 ggaaagcaaa ttagtcgact tctcggcgtt aaccgccggc cttgatatgt cgcgcttcca
     7981 tcttatggat tttggcaccc gtcgccgttt ttctcgcgaa gtacaagaaa ccatcgttaa
     8041 gcgtctgcaa caggaatcct ggttcgtggg caccagcaac tacgatctgg cgcgtcggct
     8101 ttccctcacg ccgatgggaa cacaggcaca cgaatggttc caggcacatc agcaaatcag
     8161 cccggatcta gccaacagcc agcgagctgc acttgctgcc tggctggaag aatatcccga
     8221 ccaacttggc attgcattaa ccgactgcat cactatggat gctttcctgc gtgatttcgg
     8281 tgtcgagttc gccagtcgct atcagggcct gcgtcatgac tctggcgacc cggttgaatg
     8341 gggtgaaaaa gccattgcac attatgaaaa gctgggaatt gatccacaga gtaaaactct
     8401 ggttttctct gacaatctgg atttacgcaa agcggttgag ctataccgcc acttctcttc
     8461 ccgcgtgcaa ttaagttttg gtattgggac tcgcctgacc tgcgatatcc cccaggtaaa
     8521 acccctgaat attgtgatta agttggttga gtgtaacggt aaaccggtgg cgaaactttc
     8581 tgacagccct ggcaaaacta tctgccacga taaagcgttt gttcgggcgc tgcgcaaagc
     8641 gttcgacctt ccgcatatta aaaaagccag ttaatatcat cagggagcta atcggctccc
     8701 tttttttacc tttaattccg aaatctttcg ctgcatttgc gaattctgct tgtctgattg
     8761 cagatgccag gtaacatagg tatcccccca tttacgggtg gacaagtgtt tattttttcc
     8821 gactattaac agagagaata ttatgagcgt tgtgcctgta gccgacgtac tccagggccg
     8881 cgtagccgtt gacagcgaag tcaccgtacg cggatgggta cgtacccgcc gagattcaaa
     8941 agctggcatc tccttcctcg ccgtttatga cggttcctgc tttgatcctg tacaggctgt
     9001 catcaataat tctctgccca attacaatga agacgtcctg cgtctgacca ccggctgctc
     9061 ggtcattgtg acgggtaaag tcgtggcgtc gccgggccag gggcaacaat ttgaaattca
     9121 ggccagcaag gttgaagtta ctggttgggt tgaagatcca gacacttacc cgatggcggc
     9181 aaaacgccac agcattgagt atctgcgtga agtcgctcac ctgcgtccgc gcacaaacct
     9241 gattggtgcc gtcgcgcgcg ttcgccatac gctggcgcag gcgctgcatc gcttctttaa
     9301 cgagcaggga ttcttctggg tttcaacgcc actgattacc gcatctgata ccgaaggtgc
     9361 aggcgaaatg ttccgcgttt ctacgctgga tctggaaaac ctgccgcgta acgatcaggg
     9421 caaagtggat ttcgacaaag acttctttgg taaagagtct ttcctgaccg tatctggcca
     9481 gttgaacggc gaaacctacg cttgcgcatt atccaaaatt tataccttcg gcccgacttt
     9541 ccgtgctgaa aactccaaca ccagccgtca cctggcggaa ttctggatgc tggagccgga
     9601 agtggcgttt gctaacctga acgatatcgc gggtctggct gaagccatgc tgaaatatgt
     9661 cttcaaagcg gttctcgaag aacgcgcaga cgacatgaaa ttcttcgctg aacgcgtaga
     9721 taaagatgcc gtttcacgtc tggaacgctt cattgaggcc gatttcgcgc aggtggatta
     9781 caccgacgca gtgaccattc tcgaaaactg cggcaggaag tttgaaaacc cggtttactg
     9841 gggcgtcgat ctctcttctg agcatgagcg ttatctggcg gaagaacact ttaaagcacc
     9901 ggtagtggtt aaaaactatc cgaaagatat taaagcgttc tatatgcgcc tcaacgaaga
     9961 cggtaaaacc gttgcggcta tggacgttct ggctccgggc atcggtgaga tcattggtgg
    10021 ctcccagcgt gaagaacgtc tggacgttct ggacgagcgt atgctggaaa tgggcctgaa
    10081 taaagaagat tactggtggt atcgcgatct gcgtcgctac ggtactgttc cgcattcagg
    10141 tttcggtctt ggttttgaac gcctgattgc ttacgtaact ggcgtgcaaa acgtgcgtga
    10201 tgtgattccg ttcccacgta ctccgcgcaa cgccagcttc tgattttctt cctcaaagag
    10261 ccagcgcctg ctggctcttt tttatccttt ctgtcaagtt atctgtttgt taagtcaagc
    10321 aatctatttg caaccccgcc ataacgatca tcctgttacg gaatattaca ttgcaacatt
    10381 tacgcacaaa aaataacccg cattcttatt gcggattagt tttttcttag ctgatagcac
    10441 aattttcata ctattttttg gcattctgga tgtctgaaag aggattttgt gccaggtcga
    10501 taaagtttcc a
//
LOCUS       DAPHDW010000153        10335 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.156, whole genome shotgun sequence.
ACCESSION   DAPHDW010000153 DAPHDW010000000
VERSION     DAPHDW010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10335
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.156"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            110..652
                     /locus_tag="RH057_003806"
     CDS             110..652
                     /locus_tag="RH057_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130110.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGPYVGVNASF"
     gene            752..1954
                     /locus_tag="RH057_003807"
     CDS             752..1954
                     /locus_tag="RH057_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HDV3130111.1"
                     /translation="MLKVEMLSTGDEVLHGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVVPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     IESMTLSV"
     gene            2009..2479
                     /locus_tag="RH057_003808"
     CDS             2009..2479
                     /locus_tag="RH057_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HDV3130112.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(2520..3422)
                     /locus_tag="RH057_003809"
     CDS             complement(2520..3422)
                     /locus_tag="RH057_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HDV3130113.1"
                     /translation="MTESTTSSPHDAVFKTFMFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRLMRYATAA
                     MQRHLDKGYDRVPLVVPLLFYHGETSPYPYSPNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDRDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIEEIAERSPLQKERLMTIAERLRQEGHQIGWQEGMHEQAIKIALRMLE
                     QGIDRDQVLAATQLSEADLAANNH"
     gene            complement(3616..4806)
                     /gene="glpC"
                     /locus_tag="RH057_003810"
     CDS             complement(3616..4806)
                     /gene="glpC"
                     /locus_tag="RH057_003810"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HDV3130114.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRKIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVITDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            complement(4803..6062)
                     /gene="glpB"
                     /locus_tag="RH057_003811"
     CDS             complement(4803..6062)
                     /gene="glpB"
                     /locus_tag="RH057_003811"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HDV3130115.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVTDIHSGLESLRQQAPAHPYSLLEPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRVTPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            complement(6052..7680)
                     /gene="glpA"
                     /locus_tag="RH057_003812"
     CDS             complement(6052..7680)
                     /gene="glpA"
                     /locus_tag="RH057_003812"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HDV3130116.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            7953..9311
                     /gene="glpT"
                     /locus_tag="RH057_003813"
     CDS             7953..9311
                     /gene="glpT"
                     /locus_tag="RH057_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HDV3130117.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFLYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            9316..>10335
                     /gene="glpQ"
                     /locus_tag="RH057_003814"
     CDS             9316..>10335
                     /gene="glpQ"
                     /locus_tag="RH057_003814"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HDV3130118.1"
                     /translation="MKLTLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTTDVNQLYDAL
                     YNKAGV"
BASE COUNT         2352 a         2587 c         2865 g         2531 t
ORIGIN      
        1 ttacaataag tgactagatc ccttttttcg ctcattgaat cctctgtgcc aggcagtaca
       61 atcccgcagc tttgggaatt ttgattaatt gcttgtggga aaggggataa tgaaaaaaat
      121 tgcgctagca ggtctggccg gaatgctgtt ggtttctgca tcggtcaatg caatgagcat
      181 cagcggccag gcgggtaaag aatacaccaa tattggtgtc ggttttggga ctgaatcgac
      241 gggcctggct ttaagcggta actggacaca taacgacgac gacggtgacg tcgcgggcgt
      301 ggggctgggg ttgaatctgc ctctcgggcc gttaatggcg accgttggcg gaaaaggcgt
      361 ttacaccaac ccgaattacg gcgatgaagg ttatgccgca gcggtaggag gtggtttgca
      421 gtggaaaatt ggcaacagct tccgtttgtt tggcgagtat tactactctc cggattcgct
      481 ctccagcggt attcaaagtt atgaggaagc gaatgctggc gcgcgttaca ccattatgcg
      541 tccagtcagt attgaggcgg gttatcgcta cctgaatctg tcgggtaaag acggtaaccg
      601 cgacaacgcc gtggctgacg gcccgtatgt tggggttaac gccagtttct gatcccttcc
      661 ggcgcggcat tctgtcgcgc cattcagttt cttactacgc cttatctccc tttgcgttat
      721 agtgattcga taacaaaagc aggagataac catgttaaaa gtggaaatgc tatccaccgg
      781 ggatgaagtg ttacacgggc aaatcgttga cactaacgct gcctggctgg ccgatttttt
      841 ctttcatcag gggctgccat tatctcgccg caatacggtg ggggataacc ttgatgactt
      901 agtcaccatt cttcgcgaac gtagtcagca cgccgatgtg ctgatcgtta acggcgggct
      961 gggaccgacc agcgatgatt taagcgcact cgccgctgcg acagcaaaag gtgaaggcct
     1021 ggtgctgcat gaagcctggc tcaaagagat ggaacgctat ttccacgaac gtggacgagt
     1081 aatggcaccg agcaaccgta aacaagcgga gctgcctgcc agtgctgaat ttatcaataa
     1141 cccggtaggc accgcctgtg gttttgccgt gcagcttaat cgttgcctga tgttctttac
     1201 tcccggcgta ccgtcagaat ttaaggtgat ggtcgagcac gaaatcctgc cgcgtctgcg
     1261 cgagcgtttt tctttaccac agccgccggt ttgtctgcgt ttaactacct ttggtcgttc
     1321 ggaaagcgat ctggcacaaa gcctggacac tctacaactg ccgccgggcg taacaatggg
     1381 ctatcgctct tcaatgccga ttatcgaact gaaactcacc ggaccggcaa gcgagcaaca
     1441 ggcgatggaa aaactgtggc tggacgttaa acgtgttgcc ggacagagcg tgattttcga
     1501 aggcaccgaa ggactgcccg cgcagatcag tcgcgaattg caaaaccgcc agttcagcct
     1561 gacgttgagc gagcaattca ccggtggttt attggctttg caactttctc gcgcaggtgc
     1621 tccattgctg gcgtgtgaag tggttccttc acaggaagaa accctggcgc aaactgcgca
     1681 ctggattaca gaacggcggg ccaaccattt tgccgggctg gcactggctg tttcgggttt
     1741 cgagaacgaa catctcaact ttgcgctagc cacaccagac ggcactttcg ctctgcgtgt
     1801 gcgtttcagc actacgcgct acagcctggc tatccgtcag gaagtgtgcg caatgatggc
     1861 actgaatatg ctgcgccgtt ggttaaacgg ccaggatatc gccagtgagc atggctggat
     1921 tgaggttatt gagtccatga ccttatctgt ctgattccgc cactctgtgc cggtttactg
     1981 caccggcaca ttccccctcc ggcaaaactt gtccaaccct ccctacggtg agcgtggtgt
     2041 tggggccagt gtggcacggg ctgcgcgctg gggacgcatt gagaattaca tggcgcaagt
     2101 caacgattcg ctttgtctgt tagtgcaggt ggaaagtaaa acggcactgg ataacctgga
     2161 cgagatcctc gacgtcgaag ggattgatgg cgtgtttatt ggaccagcgg atctttctgc
     2221 gtcgttgggc tacccggata acgccgggca cccggaagtg cagcgaatta ttgaaaccag
     2281 cattcggcgg atccgtgctg cgggtaaagc ggctggtttt ctggctgtgg ctcctgatat
     2341 ggcgcagcaa tgcctggcgt ggggagcgaa ctttgtcgct gttggcgttg acacgatgct
     2401 ctacagcgat gccctggatc aacgactggc gatgtttaaa tcaggcaaaa atgggccacg
     2461 cataaaaggt agttattgat atcaaaggcc catggggatc ggctgtgggc ctgtgttaat
     2521 tagtggttat tcgctgccag atcggcttcg cttagctggg tggccgcgag cacctggtca
     2581 cgatcaatgc cctgttccag catgcgcaaa gcaattttaa tggcttgttc atgcatacct
     2641 tcctgccagc caatttgatg cccttcctgc cgtagccgtt cagcaatagt cattaatctc
     2701 tccttttgta gtggtgaacg ttcggcaatc tcctcaataa aacgggtgaa acgggaggta
     2761 tcgccgcatt gcagcagata attaaacagt gtttgtagct ggctgtcatt agtgaagcct
     2821 ctaaccaaaa gcgtggtgat cctgtcgacc atgccgatta aatcgcggtc gcgaatatgc
     2881 ttttgaatca gttccagcag agctatacgc cgatgttgca tgatctcatc gtcaggtacg
     2941 atggtgatat ccaccaacgg aaaagcttcg gtgtacaact gccgggcaag ttgcggatcg
     3001 tcaaactcat ccagccagtt aggtgagtac gggtagggcg atgtttcgcc atgataaaac
     3061 agcaatggca ccaccagcgg aactctgtca tagcctttat ccaggtgacg ctgcatggcg
     3121 gcagtggcat agcgcattag ccgaaaagcc atattctttt ctgcagagct ttgatgttca
     3181 atcacgcagt agatatagcc gtcaccgtcg ctggtttcca cggaccacaa aacatccgag
     3241 tagtaagcgc gtaaactttt ttcaataaaa ctagtgggtt ccaggcgtaa ggtttgcagg
     3301 ttgcaaagct tgcgcagtgg ttctggtaaa tgtatttcga gaaaatcccg tgcggtttcg
     3361 ggtgtgaaca taaaggtttt aaacaccgca tcatgcgggg aggatgttgt tgattcggtc
     3421 atggttgtcc gtcacctgat atttatgatg acgcgacatt acgtctatcc cgggcctttg
     3481 acatctgtca gtttcagcct ttgcgagaca ttttcagagg aattttcaaa gcgttgcaac
     3541 gcatacatgg tgcgtggaat cgcgctcgca aaaatgcccg ataagcaatg tgcttaccgg
     3601 gcatcaaaag gtcggttaag ccagcgcctg ggccagtagc gtaatcggat gttcgcagcg
     3661 aaggctcgtg gacatctcaa tctgccattt gcaggtttcg cagtcggtga tgaccagatc
     3721 cgcgccgctc tcttctatct ggcggaacag tggcgcaccg atggcttgtg aggtggggta
     3781 gttctctttt ttgaaaccgt aagtacccgc aataccgcag cactgggaat ccagcaccgt
     3841 taactcaagc cccgggattt tacgcaacag ctccagggtg tagagcgtcc agcccatttt
     3901 ttccatatgg cacggagtgt gataaaccac tttcagcggc agcggtttca gcggtaacgt
     3961 tttgccttcg tccagcttgc gccacagcca gcgggttgcc agttcgatat gatcgcgcaa
     4021 gcctttgttg tcgacattca gcacttccgg gtattcgtcg cgcagggcaa atgtacaggt
     4081 tgaggaggtg gcaatcaccg gaatgccttt tactcccaca gcttcgcgga tcgactctac
     4141 attcgtaatt gcctgtttgc gtgctttatc ggtaaagccg ttggcgatta gcggtacgcc
     4201 gcagcatttt tctttgctga gcagttgtac accggtaccc attgcgttga gcactttaat
     4261 taaatcttta cctaactgcg gatggttgta gttaacgaag cagccgtgaa agaaagcgac
     4321 ctggtcttta tattgtgctt gctgagccgc tatgctgcga taccagcgac ggaacgtgcc
     4381 gaaggagtat ttcggtagcg tgcggcgatg atcgattttt aacgccgcat caagcagctg
     4441 ccgcaccggt ttcagcgagg tagcggtgtt gacgattggt gcgaacggcg tggaaacgct
     4501 acccatcagg tcggtatgac tcaacacaaa attacgcagc gacgggcgcg tggtgtcata
     4561 ttttgcccgc gcgcgctgga taatatcgcc aatcttcaca tcggacgggc aggcgacttc
     4621 acaacgtttg cagttgatgc aatatttcag cgcctcgtca tacagtgcgc catctttcaa
     4681 acgcagacgc tcgccatccg gcccggcttg ttttggccct ggataaccgg gattcacccg
     4741 gctcaccggg caggcggtgg tgcagacggt gcacttaatg cagttttcga agctggtgtc
     4801 gttcattgtt ggcctcctgc gcgttgggca atctgttgag cggcatgtaa agcactgacg
     4861 gcacaaacac cgccgccgca tccctgggcg atgggatcaa atccgcccag caccgaaccg
     4921 atggcaaaca ggttctcaat ggtttgccct gcctgcgacg ggcgtagcgt ctcatcagtg
     4981 gttacaccga actgctgcca cggttgcggc gcaaaaaaat cgcccttata ccattcaccc
     5041 cgcgtggcgg tttgtagcac atcaaggccg agaatcggct cgcgaatgcc gttacgttcc
     5101 gctaccagtc cgccactaaa gaaactgccg ctggcgagaa ccgcgaaacg tggacgtagc
     5161 ggaatatcgg cgtgattgcg ggtccagatt tcattcacta cgccattttt acaggtcact
     5221 tttttcactt catcgcccgg catccacacg ccaccctggc gcacaaactg gcgctgtaac
     5281 tggttttgca gacgaatgcc cagcacggaa ggcggcagcg ttggcaaaag catcagtgaa
     5341 caaggtagtt tttcattcaa ccaacgccac agtttgtcat cggccaggcc gaagcaggcg
     5401 ggcatcagga tcatttcgca ggtattggcg acaggaataa gcgcatcaag taacagcggc
     5461 cagttttctt cattatcaag gaaacgggcg atattcacag cgcgaaattc ggtggcgtta
     5521 ttgcgcagga catccagttc cggcagctct atttctgcgg tttcaaccga aagatccagt
     5581 tcgcgcaacg aagccgccgc aagatgcgcc tggaaatcca tcagcccgct tatccccact
     5641 acacatatct tcttcgcggg tagcggccag acgggaactt ctggcgaact taaccaggtt
     5701 gcacgcaaag tgccgagcgg ggtaacgcgc tgatgggcca gttccacgct gccctgcaat
     5761 tgcgcaccgc tttcagcgat caatgcctgc gcctggcaag cgagatcgag cacgcgttgt
     5821 ggttcgagaa gactgtaagg atgggctggt gcctgctgac gcaaagattc cagtccactg
     5881 tgaatgtctg tcaccggttg accatcaggc agatggctca gcaaatccag cgaaccggat
     5941 gagaaatgca gcgcgctttg accacgcgtg acaatggcac agcgcaggcc gtgtttttgc
     6001 agttgcaggc cacagagtaa tccggcgagg ccgccgccca taatgacagt atcaaagcgc
     6061 atctttctgc tccttctcca gaccacacag tccctgataa acccagcggg taaattcgct
     6121 ttcgcgcagt gcgtctcccc aggcgatggg ttgcacgcct ttccagcgtt cgttaaggaa
     6181 ggtggaaagt tgctcgatag attgcgcgga cgtcgtgacg ttaaaacgtt gcagcagtcc
     6241 ggcagcgcgg caggcgcaga gttcgccctg gcaggtaccc atccccacac gggtacgacg
     6301 gcgtaaatcc agcaggctat taacgtttaa attttctacc gcgtactgaa cttcacccgc
     6361 agttaccgct tcgcactcac atactaggct acgatgcaga cggccttcgc tcagccaggc
     6421 aggcgtgcga tcgccatgac gataaaccgc agaaccgcgc agcggggcag gcagggagat
     6481 gactttacgc aaggtaactt cagcgggttc ttgtgaacca ggcagtgcca gatcggcagt
     6541 cgtacagggg cgcgtgttgc ccagtttgcg gcataccgcg tcggtagccc attcagccat
     6601 cagccgatag gtcatcagtt tgccaccggt gatggtgata aatccgtcca gaccatcgcg
     6661 ttcagcatgg tcgagcagca cgatgccacg gctgacgtta cgtccgctcg ggtcgtcatc
     6721 gctggcaacc agcgggcgca cgccagaata ggcccgcaaa atgcgcgttt tcgccatcac
     6781 gggggccagt ttttcccctt cacgcagcag aatatcaacc tcttctgccg tcactcgatt
     6841 atcgtcaatc tcgttgtaat caatacgtaa agaggtggtg ccaatcagcg aaatggtatc
     6901 gccaggcacc agaatatcgg cgtcggaagg tttacggcag cggttgatca catgctggtt
     6961 aatgcggtga tccatgatca gcagcgatcc tttcgccggg aacatgcgaa tgcgcagatc
     7021 ggcatattcg gcaatgtgtt gcccccagat cccagcggca ttaaccacga caggtgcatg
     7081 aagggcctga gtttcgccgg tgagatggtt acgtacacga acaccgcaca ccgtcgcgcc
     7141 ttcacgaatc agccccgtga cttcatgagc ggtaaggata acggcaccgt gttctttggc
     7201 atccagcatg tttgctgcgg tcagacgaaa tggatcaacg gtgccatccg gaactttcac
     7261 cgcgccaatc agtgccgggt taacggcagg ttcgataatg cgcgcttgct gcgggtctat
     7321 agcttctgcg ctgatccctg cttcttcgca ggcgcgaata aaagtggcct ggaatgagag
     7381 gtcatcttcc ggcagggtga taaacaggcc gttggttggt tcaacgcagt gacgtgcaat
     7441 gcgtttcagg atctggtttt cactaatgca ttcgcgggcc gattccgcat cggttaccgc
     7501 atagcgcgca ccgctgtgca gcagaccgtg gttacgcccg gtggcaccgg ttgcgatgtc
     7561 gtggcgctca accaaaatca cgcgcagccc gcgcagggca cagtcgcggg caatcccggc
     7621 tcccgttgcg ccgccaccaa tgataatcac gtcacttgat tgcgagtcgc gagttttcat
     7681 tgttatccct ctgaagttcg ttttttacca tttagccata gtaaaaacat gaattgtttg
     7741 atttcgcgca tattcgctca taattcgaaa gtgaaacgtg atttcatgcg tcattctgaa
     7801 cattttgtaa atcttattta ataatgtgtg cggcaattca catttaattt ctgaatgttt
     7861 tcttaacatc gcggcaacta aagaaacggc aggttctccc actgaatcag tctgttaatc
     7921 ataaataaga ccacgggcca cggaggcttt caatgttgag tatttttaaa ccagcgccac
     7981 acaaagcgcg cttacctgct gcggagatcg atccgactta tcgtcgattg cgctggcaaa
     8041 ttttcctggg gatattcttt ggctatgcgg cttactattt ggttcgtaag aactttgcgc
     8101 ttgctatgcc ttatctggtc gagcagggat tctctcgcgg tgatttaggt ttcgcgcttt
     8161 cggggatctc gatcgcttat ggattttcga aattcataat gggttcggta tcagatcgct
     8221 cgaatccgcg cgttttcctc cccgcaggtt tgattctggc ggcggcagtg atgttgttta
     8281 tgggctttgt gccatgggcg acgtcgagca ttgcggtgat gtttgtactt ttgttcctct
     8341 gcggttggtt ccaggggatg gggtggccgc cgtgtggtcg tactatggtg cactggtggt
     8401 cgcagaaaga acgtggcggc attgtgtcag tgtggaactg tgcgcacaac gttggtggtg
     8461 gtattccgcc gctgctgttc ctgctgggga tggcctggtt caatgactgg cacgcggcgc
     8521 tctatatgcc cgctttctgc gccattctgg tggcattatt cgcctttgcg atgatgcgcg
     8581 ataccccgca atcctgtggc ttgccgccga tcgaagagta caaaaatgat tatccggacg
     8641 actataacga aaaagcggaa caggagctga cggcgaagca aatcttcatg cagtacgtac
     8701 tgccgaacaa actgctgtgg tatatcgcca tcgccaacgt gttcgtttat ctgctgcgtt
     8761 acggcatcct cgactggtca ccgacttatc tgaaagaggt taagcatttc gcgctggata
     8821 aatcctcctg ggcctacttc ctttatgaat atgcaggtat tccgggcact ctgctgtgcg
     8881 gctggatgtc ggataaagtc ttccgtggca accgtggggc aaccggcgtt ttctttatga
     8941 cactggtgac catcgcgact atcgtttact ggatgaaccc ggcaggtaac ccaaccgtcg
     9001 atatgatttg tatgattgtt atcggcttcc tgatctacgg tcctgtgatg ctgatcggtc
     9061 tgcatgcgct ggaactggca ccgaaaaaag cggcaggtac ggcagcgggc tttaccgggc
     9121 tgtttggtta cctgggcggt tcggtggcgg cgagcgcgat tgttggctac accgtggact
     9181 tcttcggctg ggatggcggc tttatggtaa tgattggcgg cagcattctg gcggttatct
     9241 tgttgattgt tgtgatgatt ggcgaaaaac gtcgccatga acaattactg caaaaacgca
     9301 acggaggcta atggcatgaa attgacgctg aaaaacctta gcatggcgat catgatgagc
     9361 actatagtca tgggaagcag tgcaatggcg gcggacagca acgaaaaaat agtcatcgcc
     9421 catcgcggtg ccagtggata tttgccggag catacgctgc cagcaaaagc gatggcgtat
     9481 gcgcagggag cggattatct ggaacaggat ttggtgatga ccaaagacga ccatctggtt
     9541 gttctgcatg accattatct cgatcgtgtt actgatgttg ccgatcgttt cccggatcgg
     9601 gcgcgcaaag acggtcgtta ctacgcgata gatttcacgc tggatgaaat taagtcgctg
     9661 aaatttaccg aaggtttcga tattgaaaac ggtaaaaaag tgcagactta tccggggcgt
     9721 ttcccaatgg gtaagtccga cttccgggtg cacacctttg aagaagagat tgaatttgtt
     9781 caggggttaa atcactctac cgggaaaaat atcggtattt atccagaaat caaagcgccg
     9841 tggttccatc atcaggaagg gaaggatatt gcggcaaaaa cgctggaagt gctgaagaaa
     9901 tatggttaca ccggtaaaga cgataaagtt tatttgcaat gttttgatgc tgatgagctg
     9961 aagcgtatta agaatgagct ggaacccaaa atgggcatgg agctcaatct ggtacagctg
    10021 attgcctata ccgactggaa tgaaacgcag cagaaacagc cggatggaag ctgggttaat
    10081 tacaactacg actggatgtt taagccgggt gccatgaaac aggtggcgga atatgcagat
    10141 ggtattggtc cggattacca tatgttgatt gaggaaacat cgcagccggg taatatcaaa
    10201 ctcactggca tggtgcaaga tgctcagcag aataaactgg tagtgcatcc ttataccgtg
    10261 cggtcggata aactgcctga atacactact gatgtgaatc agttatatga tgctctgtat
    10321 aacaaagcgg gtgta
//
LOCUS       DAPHDW010000154        10188 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.157, whole genome shotgun sequence.
ACCESSION   DAPHDW010000154 DAPHDW010000000
VERSION     DAPHDW010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10188
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.157"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(4..621)
                     /locus_tag="RH057_003815"
     CDS             complement(4..621)
                     /locus_tag="RH057_003815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HDV3130119.1"
                     /translation="MIKVYGVPGWGSTISELMLTLADIPYQFVDVSGFDHEGASRELL
                     KTLNPLCQVPTLALENDEIMTETAAIALMVLDRRPDLAPPVGRAERQQFQRLLVWLVA
                     NVYPTFTFADYPERWASDAPEQLKKNVIEYRKSLYIWLNSQLTAEPYAFGEQLTLVDC
                     YLCTMRTWGPGHEWFQDNAQNISAIADAVCQLPKLQEVLKRNEII"
     gene            888..2387
                     /gene="ansP"
                     /locus_tag="RH057_003816"
     CDS             888..2387
                     /gene="ansP"
                     /locus_tag="RH057_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparagine permease"
                     /protein_id="HDV3130120.1"
                     /translation="MSKHDTDTSDQHAAKRRWLNAHEEGYHKAMGNRQVQMIAIGGAI
                     GTGLFLGAGARLQMAGPALALVYLICGLFSFFILRALGELVLHRPSSGSFVSYAREFL
                     GEKAAYVAGWMYFINWAMTGIVDITAVALYMHYWGAFGGVPQWVFALAALTIVGTMNM
                     IGVKWFAEMEFWFALIKVLAIVTFLVVGTVFLGSGQPLDGNTTGFHLITDNGGFFPHG
                     LLPALVLIQGVVFAFASIEMVGTAAGECKDPQTMVPKAINSVIWRIGLFYVGSVVLLV
                     MLLPWSAYQAGQSPFVTFFSKLGVPYIGSIMNIVVLTAALSSLNSGLYCTGRILRSMA
                     MGGSAPSFMAKMSRQHVPYAGILATLVVYVVGVFLNYLVPSRVFEIVLNFASLGIIAS
                     WAFIIVCQMRLRKAIKQGKAADVSFKLPGAPFTSWLTLLFLLSVLVLMAFDYPNGTYT
                     IAALPIIGILLVIGWFGVRKRVAEIHSTAPVVEEDEEKQEIVFKPETAS"
     gene            complement(2502..3563)
                     /locus_tag="RH057_003817"
     CDS             complement(2502..3563)
                     /locus_tag="RH057_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HDV3130121.1"
                     /translation="MHLRHLFSSRLRGSLLLGSLLVASSFSTQAAEEMLRKAVGKGAY
                     EMAYSQQENALWLATSQSRKLDKGGVVYRLDPVTLEVTQAIHNDLKPFGATINNTTQT
                     LWFGNTVNSAVTAIDAKTGEVKGRLVLDDRKRTEEVRPLQPRELVADDATNTVYISGI
                     GKESVIWVVDGENIKLKTAIQNTGKMSTGLALDSKGKRLYTTNADGELITIDTADNKI
                     LSRKKLLDDGKEHFFINISLDTARQRAFITDSKAAEVLVVDTRNGNILAKVAAPESLA
                     VLFNPARNEAYVTHRQAGKVSVIDAKSYKVVKTFDTPTHPNSLALSADGKTLYVSVKQ
                     KSTKQQEATQPDDVIRIAL"
     gene            3805..5907
                     /locus_tag="RH057_003818"
                     /pseudo
     CDS             3805..5907
                     /locus_tag="RH057_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415968.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
     gene            complement(5943..6608)
                     /gene="mcbR"
                     /locus_tag="RH057_003819"
     CDS             complement(5943..6608)
                     /gene="mcbR"
                     /locus_tag="RH057_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HDV3130122.1"
                     /translation="MPGMGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVGKRQLDEINRIRYELELMAVALAVE
                     NLTPQDLAELQELLEKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWV
                     RMGPGLHYLYEAINPAELREHIENYHLLLAALKAKDKEGCRHCLAEIMQQNIAILYQQ
                     YNR"
     gene            complement(6806..7843)
                     /gene="curA"
                     /locus_tag="RH057_003820"
     CDS             complement(6806..7843)
                     /gene="curA"
                     /locus_tag="RH057_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HDV3130123.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATG
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHAIEVLGFDVCLDHHADDFAEQLVKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPGPDRLPLLMATVLKKR
                     IRLQGFIIAQDYGHRIHEFQKEMGQWVKEDKIHYHEDITDGLENAPQTFIGLLKGKNF
                     GKVVIRVAGDD"
     gene            8027..8545
                     /gene="mddA"
                     /locus_tag="RH057_003821"
     CDS             8027..8545
                     /gene="mddA"
                     /locus_tag="RH057_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HDV3130124.1"
                     /translation="MSIRFARKADCAAIAEIYNHAVLYTAAIWNDQTVDADNRIAWFE
                     ARTIAGYPVLVSEEDSVVTGYASFGDWRSFDGFRHTVEHSVYVHPDHQGKGLGRKLLS
                     RLIDEARDCGKHVMVAGIESQNQASLHLHQSLGFVVTAQMPQVGTKFGRWLDLTFMQL
                     QLDERTEPDAIG"
     gene            8542..8991
                     /locus_tag="RH057_003822"
     CDS             8542..8991
                     /locus_tag="RH057_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3130125.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMVRITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLMLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(8992..9225)
                     /locus_tag="RH057_003823"
     CDS             complement(8992..9225)
                     /locus_tag="RH057_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HDV3130126.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(9311..9484)
                     /gene="ortT"
                     /locus_tag="RH057_003824"
     CDS             complement(9311..9484)
                     /gene="ortT"
                     /locus_tag="RH057_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HDV3130127.1"
                     /translation="MSLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            9679..9774
                     /gene="yncL"
                     /locus_tag="RH057_003825"
     CDS             9679..9774
                     /gene="yncL"
                     /locus_tag="RH057_003825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HDV3130128.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
BASE COUNT         2459 a         2492 c         2479 g         2758 t
ORIGIN      
        1 cactcaaata atttcattcc tttttaatac ttcttgtaat ttcggtaact gacatacagc
       61 atcagcgatc gcgctgatat tctgcgcatt atcctgaaac cattcatgcc ccggccccca
      121 tgtgcgcatg gtgcagagat aacagtccac cagcgttagt tgctcaccaa aggcataagg
      181 ttcagcggtc aattgtgaat tcagccagat ataaagtgat ttgcgatatt caattacatt
      241 cttctttaac tgttcaggtg cgtcagatgc ccaacgctca gggtaatcgg cgaaagtgaa
      301 tgttggatag acattagcaa ccagccagac caatagccgt tgaaactgct gacgttcggc
      361 gcgcccaacc ggtggcgcaa ggtccgggcg acgatcgaga accatcaatg caatcgccgc
      421 tgtctccgtc ataatctcgt cattttccag cgccagtgtc ggaacctgac acaacgggtt
      481 cagggttttc aataactcgc gtgaagctcc ctcgtggtca aaaccactga catcaacgaa
      541 ctgataagga atatcagcca gtgttagcat cagctcactg attgtagagc cccatccggg
      601 tacgccatac accttaatca tatgcccctc acaatctttt aaagattaag tgtaggacag
      661 tacacaggtt aaccagcaaa aggatttgtc attttttcgt cagttgtatt aatccccttc
      721 aggattgtta ggggatgttt tgcatcgttt atcgataacg ggtaggatgc tgcgccgata
      781 gttttatacc cataaagaat aatggtgata actatcatcg ccaggatgaa taaacattgt
      841 tcatggcaac ttatatgact ttttcattaa agcaatcagg gagagcaatg agtaaacacg
      901 acaccgacac ttcagatcaa cacgccgcga aacgccgctg gcttaatgcc cacgaagagg
      961 ggtatcacaa agcgatgggc aatcgccagg tacagatgat cgccattggc ggcgcgattg
     1021 gcaccggctt gtttttaggt gcaggagccc gactgcaaat ggcgggtcca gcattggcac
     1081 tggtttattt aatttgtggc ttgttttcgt tttttattct gcgtgcattg ggtgagctgg
     1141 tgctacaccg cccttccagt ggcagttttg tctcttatgc ccgtgagttt ttgggtgaga
     1201 aagccgctta tgttgctggc tggatgtact tcatcaactg ggcgatgacg gggattgttg
     1261 atattaccgc cgtcgctctg tatatgcatt actggggtgc gtttggcggc gtgccgcagt
     1321 gggtctttgc gctcgctgca cttaccatcg ttggcaccat gaatatgatc ggtgtgaaat
     1381 ggtttgcgga gatggagttc tggtttgcgc ttattaaagt gctcgccatt gtgacctttt
     1441 tggtcgtggg tacagtgttc ctcggtagtg gtcagccgct ggatggcaac accactggct
     1501 ttcatttaat taccgataat ggcggcttct tcccccacgg tttgctgcct gctctggtgt
     1561 tgattcaggg cgtagtgttt gcttttgcct ccattgaaat ggtgggtaca gctgccggag
     1621 aatgtaaaga tccgcagacc atggtgccta aagcgattaa cagcgtgatt tggcgtattg
     1681 gcctgtttta cgtcggctcc gtggtgttgc tggttatgct attgccgtgg agcgcgtatc
     1741 aggcggggca aagtccgttc gtgacgtttt tctctaaact gggtgtgcca tatatcggca
     1801 gcattatgaa cattgtggtg ctgaccgctg ccctctccag cctgaactca ggtctgtact
     1861 gcaccggacg tattctgcgc tcaatggcga tgggcggttc cgcaccgagt tttatggcga
     1921 aaatgagtcg tcagcatgtg ccgtatgccg ggattctggc gacactagtt gtgtatgtcg
     1981 tcggcgtatt cctcaactat ctggtgccgt cgcgcgtatt tgagattgtg ttgaacttcg
     2041 cgtcgctggg aatcatcgct tcatgggcgt ttatcatcgt gtgccagatg cgcctgcgta
     2101 aagcaattaa acaaggcaaa gcagcggatg tcagttttaa actgcctggc gcgcccttca
     2161 cttcctggct gacattactg tttttactga gtgtcctggt gctgatggcg ttcgattacc
     2221 cgaacgggac ttatactatc gcggcgctgc cgattatcgg tattctgctg gttataggct
     2281 ggtttggtgt gcgcaaacgc gttgctgaaa ttcacagcac tgcgccagtc gtcgaggaag
     2341 atgaagaaaa acaggaaatt gtgtttaagc ctgaaacggc gagctaattg caaaattgcc
     2401 cggctacgtg ccgggcaaat ttagattact gacgaaggtg tgccgcatgc ggcgtgataa
     2461 aagtcaggca agtctctccg ttttcaccct cgccacatct attacagcgc aatacgaatc
     2521 acatcgtccg gctgggtagc ttcctgctgt ttagtggatt tttgtttcac actgacatac
     2581 agcgttttgc catcggcaga cagcgccagg ctgtttggat gagtcggcgt atcgaacgtt
     2641 ttcaccactt tatagctttt cgcgtcaatc acactgactt tacctgcctg acgatgcgtt
     2701 acgtaggctt cattacgcgc tgggttaaac agcacagcca gtgattctgg tgccgcaacc
     2761 ttcgccagaa tattgccatt acgggtatcg accactaaca cttctgcggc tttagaatcg
     2821 gtgataaatg cacgctgcct ggcggtatca aggctaatgt tgataaagaa gtgctctttg
     2881 ccgtcatcca gcagcttttt acggctgagg attttattgt cggcggtgtc gatggtaatc
     2941 aattcgccgt cagcgttagt ggtgtaaaga cgtttgcctt tgctatccag cgccagaccg
     3001 gtactcattt taccggtgtt ctggatggcg gttttcagtt tgatattctc gccatcaacg
     3061 acccaaatca cgctctcttt accaatacca ctgatgtaaa cggtgttcgt ggcatcatca
     3121 gctaccagct cacgcggttg cagcgggcgc acctcttccg tgcgcttacg atcatccagc
     3181 accagacggc ctttcacctc gcccgttttg gcatctatcg ccgtgaccgc gctgtttacg
     3241 gtgttaccaa accacaacgt ctgagtcgtg ttattgatgg tggcaccaaa cggcttgaga
     3301 tcgttatgga tcgcctgcgt cacttccaga gtgaccggat caagacgata aaccacgcca
     3361 cctttatcca gtttgcggct ttgcgaagtg gcgagccaca gtgcgttttc ttgctggcta
     3421 taagccattt cgtaggcacc tttacctacc gctttacgca gcatttcttc tgcggcctgc
     3481 gtactgaatg atgaagcaac aagcaatgaa cctaacagta atgaaccacg caggcgcgat
     3541 gaaaacagat gacgtaaatg catgacgact ccctttgatg aaccgatatt tttgctgctt
     3601 cacaatgctt cgctaagcgt aatcatcgtt acgctcttgt tataaatggg aatggtaatc
     3661 attattttct caaatgtgga gcatttcttg ccgttacgac tacaattttc tcaattactt
     3721 aacgtatcgt gccgtttcgt tttccataaa tttacaattt cattaccata acggcgtttt
     3781 cccattctta acctggcttt tttcatgaag attttttccg tccgacagac cgttttgccc
     3841 gcactgcttg tcctttcccc cgttgttttt gccgctgatg aacagactat gattgtcagt
     3901 gccgcaccgc aggtggtttc agaactggat accccagcag cagtaagcgt ggtggatggc
     3961 gaggagatgc gcctggcaac accgcgcatt aacttgtccg aatcactgac cggcgtgcct
     4021 ggtttgcagg tacaaaaccg gcagaactat gcgcaagatt tacagctgtc gattcgcgga
     4081 tttggctccc gctccactta cggtattcgc ggtattcgcc tgtatgtgga cggtattccc
     4141 gccaccatgc ccgacgggca agggcaaaca tccaacatcg atttaagcag tgtgcaaaat
     4201 gtggaagtgc tgcgtggccc cttctctgcc ctgtatggca acgcgtctgg tggggtaatg
     4261 aatgtcacca cccagaccgg acaacagcca ccaaccattg aagccagtag ttactacggc
     4321 agttttggca gctggcgcta tgggctgaaa gcaacgggcg caacgggaga cggcacacag
     4381 cctggcgatg tcgattacac cgtctcaacc acgcgtttta cgacccacgg ctatcgtgac
     4441 catagtggcg cacagaaaaa tttagctaat gccaaactgg gcgtacgcat tgatgaagcc
     4501 agcaaattaa gtctgatttt caatagtgtg gatatcaaag cagatgaccc aggtgggcta
     4561 accaaagcag aatggaaggc taatccacaa caagcgcctc gtgcagaaca gtacgacacg
     4621 cgaaaaacca tcaagcaaac tcaggctggg ttgcgctatg agcgtagcct gagcgcgcaa
     4681 gatgatatga gtgtgatgat gtatgccgga gagcgagaaa cgacccagta ccagtcaatc
     4741 ccgatggccc cccaacttaa cccgtcacat gcgggcggcg tgattaccct gcaacgccat
     4801 taccagggga tagacagccg ctggacacac cgtggtgaac tgggcgttcc ggtcacgttc
     4861 actaccggcc tgaactacga aaacatgagt gaaaaccgca agggctacaa taacttccgc
     4921 ctgaatagcg gcatgccgga gtacgggcaa aaaggtgagt tgcgtcgcga cgaacgcaat
     4981 ctgatgtgga acatcgatcc ctatttacag acgcagtggc agctgagcga aaaactgtcg
     5041 ctggatgctg gcgtgcgcta cagctccgtg tggtttgatt ccaacgacca ttacgttact
     5101 ccgggtaacg gcgatgacag cggtgatgcc agttatcata aatggctacc tgccggttcg
     5161 ttaaaatatg caatgaccga tgcctagaat atctatctgg cagccgggcg aggttttgaa
     5221 acgccgacga ttaatgagct gtcttatcgt gctgatgggc aaagcggtat gaactttggt
     5281 ttaaaaccat ccaccaacga tacaattgag atcggcagta aaacgcgtat tggtgatggg
     5341 ctgcttagtc tcgcattgtt tcagaccgac actgatgatg aaattgttgt cgatagcagt
     5401 agcggtgggc gtacgactta caaaaatgcc ggaaagaccc gtcgtcaagg cgctgaactg
     5461 gcatgggatc aacgtttcgc aggagatttt cgcgtaaacg cgtcctggac ctggcttgat
     5521 gcgacctatc gcagcaatgt ttgcaatgaa caggattgta acggtaatcg gatgccaggg
     5581 atcgcccgta atatgggctt tgcgtcgata ggttatgtac cggaagatgg ttggtatgca
     5641 ggcacggaag cgcgttatat gggcgatatt atggcagatg atgaaaatac ggcaaaagcg
     5701 ccgtcttata ctctcgtcgg cttattcacc gggtataaat acaattacca caatttaact
     5761 gtggatttat ttggtcgtgt cgataattta ttcgataaag aatacgttgg ttctgtcatt
     5821 gtcaatgagt caaacgggcg atattacgaa ccttcgcccg gacgaaatta tggtgtcggc
     5881 atgaatattg cgtggagatt tgagtaatta ccttgatgcc cggtatttgc cgggcattta
     5941 ctttaacgat tgtattgctg gtataaaata gcaatatttt gttgcataat ttcagcaaga
     6001 caatgtctgc atccctcttt gtcttttgct tttaacgcag cgagtaacag atgatagttt
     6061 tctatatgct cccgtaattc cgctggatta atcgcttcat agagataatg taaaccaggc
     6121 cccatcctga cccacagttg ctcaatcatc tcacacagga tgggcatatt tgagcgatga
     6181 taaatcgcta agcgaaatag cctgtttaca ttaatgattt gttccatgtc gcccttttct
     6241 tgcgcctgct gtaatttctc gagcaattcc tggagttccg ccaggtcttg cggggtgagg
     6301 ttttcaacag ccagagcaac tgccattaat tccagttcgt agcggatccg attgatttcg
     6361 tctaattgac gtttccccac ttccggaact gtaaatgctt gtgcaggtgc gacagaaagc
     6421 gcattcaccg aaaccagacg taataatgct tcacgcacag gtgtaatact catacctaat
     6481 tgctccgcca gatttttagt aatcaggcgt gcgccaggtt ttaatgcgcc aatactaagc
     6541 tgatgtttca ggtcgttctc aacctgcaga gtcaaactga catgtttcat ttttcccatt
     6601 ccaggcattt taattccttc gcgtgttttc tgtccaaccc tgcctgcacg gcagaaggag
     6661 ttcacctggt gtattaaagt gaataatcat aaaccaccca cctttgttgt tattagcagc
     6721 agaacaatga cgtggttagt taattaaagc tgaaaataag ctttttgcct ggatttttat
     6781 ggcggcttac gccgccgttg aaactttaat catcacccgc gacgcggatc accactttgc
     6841 cgaagttttt acccttcagc aggccgataa acgtctgtgg cgcattctct aaaccatcag
     6901 taatatcttc gtggtagtgg attttatcct ctttcaccca ttgccccatc tccttctgaa
     6961 actcatggat gcggtgacca taatcctgag cgataataaa cccttgcaag cgaatacgtt
     7021 ttttcagcac cgttgccatc aacaggggta aacgatccgg accgggtggt agctctgtag
     7081 cgttatagct gctcactaag ccacaaacgg gaatgcgcgc agatgtatta agtaatggta
     7141 gcactgcatc aaatacctta ccgcccacgt tttcataata gatatcaata ccttttgggc
     7201 acgctttcac cagttgttcg gcaaaatcat ccgcgtggtg atcaagacaa acatcgaagc
     7261 ccagcacctc gatagcatgg cggcattttt ccgcgccacc ggcaaccccc accaccctgc
     7321 aacctttaag tttgccgatt tgccccaccg ttgctcccac aggacctgtc gccgcagcta
     7381 ccaccaacgt ttcgccctct ttaggctgac cgatatccag cagccccata taagcggtaa
     7441 agcctggcat ccccagcaca cccagcgacc acgatggatt ttgcggatga tcgccaagtt
     7501 tcaccagatc atcaccactg gatatgtcat agtcctgcca tccactgtag cccagcaccc
     7561 agtcgccagg ctgataatca ggatgattcg actccacaac acggctcacc gtgccgccga
     7621 ccatcacgcc gccaatatca acaggcggtg aataagatgg ctcatcgctc atacgtccac
     7681 gcatatacgg gtccagggac aaataaactg tgcgcagtaa cacctgtcct tcacccggtg
     7741 tggcgacatc atcttcttca agacggaaat tctccggaac cggtgcgcca tgtggacgcg
     7801 aggccagaac ccaacggcgg ttacgctgct tttgttgccc cataatgctc tcctgtgctt
     7861 ttggcatgaa tccatcaata gtagcaggct cgcaggattg gttttgtttg cagccgtcac
     7921 atcccctctg caacacgcaa taatttcatc gaggaatccg ttgcttcggg ctgttttcga
     7981 tagtattgat aacatcatca tcatcattat tttattgagg ccagacatgt ccatccgttt
     8041 tgcccgcaaa gccgactgtg ctgccattgc ggaaatttat aaccacgccg tgttgtatac
     8101 ggcggctatc tggaatgacc aaacggtgga tgcagataat cgcattgcct ggtttgaagc
     8161 gcggaccata gcaggttatc cggtgctggt aagtgaagaa gatagcgtag tgacgggata
     8221 tgcctcgttt ggcgactggc gtagtttcga tggttttcgc cataccgtgg aacattcggt
     8281 ttatgtccat cccgatcatc agggcaaagg tctggggcgt aaattgttaa gccgattgat
     8341 tgatgaagcg cgggattgcg ggaagcatgt catggtcgcc gggatcgaat cgcaaaatca
     8401 ggcctcgctg catctgcacc agtcgctggg atttgtcgtc accgcacaaa tgccgcaggt
     8461 gggcactaaa tttgggcgct ggctggatct gacatttatg cagttgcaac tcgacgaacg
     8521 cactgaaccg gacgcgattg gatgaatcag tcgctcaccc ttgctttttt gattgccgcc
     8581 ggaattggtc tggtagtcca gaacacgctg atggtgcgca tcacccagac ctcctctacc
     8641 attctcatcg ccatgttgct gaactcactg gtggggattg tgctctttgt ttctatttta
     8701 tggtttaaac aaggcatggc ggggtttggc gaactggtgt ccagcgtgcg ctggtggaca
     8761 cttatacccg gcttgctggg atcgtttttt gtcttcgcca gtatcagcgg atatcagaat
     8821 gtgggagcgg cgacaactat tgcagtactg gtcgccagcc agctaattgg cggtctgatg
     8881 ctggatatct tccgcagtca tggcgtacca ctacgggcgt tgttcggccc aatttgtggc
     8941 gcaattttac ttgtggttgg cgcctggttg gtagccagac gctcatttta atcagaggat
     9001 ggtgccgcct ttggttaact gttcgtggcg cgcttccata tcttctttat gcttgcgacc
     9061 gtgatgagca atagcggtgc gcaaacgctg ctgctgggtg tagcgatctt cccgacttaa
     9121 ctccgcgtca tcgctcaggg cgattaataa ttcgttcata tgggcaatga cgctctcttc
     9181 aatcgcggca tccacgcgcg cgatgacttc atccagatgc gacatacttt ctccttaacc
     9241 acttgtgaaa tttatcgatt catccaaatg atataagtct gcgtggtgcc gtcaagccag
     9301 tgagtaagat ttaaaacagt gaaaacaaca gcgccaccgg gaaactcatc ggccatgttg
     9361 cccccaccag aaaggcgctt aagaaacgga tgcgtttctt atcgtgagaa agaaaccagg
     9421 tgataagaaa tgcgattgct gccataaccg cataaaaaac aagcatatgt tgatagagag
     9481 acatgtacgg gcgtccagcc agatgaataa aacgcgcgca aatatgcgtc attttttgac
     9541 gtgcatcaag ttttttcacc agtttgagaa aaaatcatca aacagcaact tgcccttatc
     9601 catcttcaga atgagtatta ttgcggattt tcttaacccg tactatacac ataggggcaa
     9661 tgataaaagg tggcaaaaat gaatgtttcc agtagaactg tagtactgat aaatgtcttt
     9721 gctgctgttg gtttgtttac tcttatctct atgagatttg gctggtttat ttgatgtagt
     9781 gattttgcac gcagcacgta ctgtcagaga aatcaactct ctgacagtac gcaagatctt
     9841 ctcatactct ccaggtttac aacattatcc ttaatattta cttctttttg ctggcatcta
     9901 cattgaaatg agtagaaaac atccccctac tgtcgtctat acaaacagct tactgattca
     9961 aacggaatct tgttttcaca tttttctatc atgaatactt tatacgcgcg ggagtctata
    10021 aatgctttat actttcaagg aataacgtaa atataaaaag atggcgtaaa gtatatgcat
    10081 ggcaggatgt ttgcaacctc cttgatgacg ctgccccatt gaaattgcaa accccgccct
    10141 ggttatcttg cccatttatc atgcaaaaca gataattata ggcctctg
//
LOCUS       DAPHDW010000155         9965 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.158, whole genome shotgun sequence.
ACCESSION   DAPHDW010000155 DAPHDW010000000
VERSION     DAPHDW010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9965)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9965)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9965
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.158"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..143)
                     /locus_tag="RH057_003826"
     CDS             complement(<1..143)
                     /locus_tag="RH057_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130129.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWPCG
                     EFFS"
     gene            221..1315
                     /gene="ddlA"
                     /locus_tag="RH057_003827"
     CDS             221..1315
                     /gene="ddlA"
                     /locus_tag="RH057_003827"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HDV3130130.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(1339..1551)
                     /locus_tag="RH057_003828"
     CDS             complement(1339..1551)
                     /locus_tag="RH057_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HDV3130131.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGLLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            1811..2119
                     /locus_tag="RH057_003829"
     CDS             1811..2119
                     /locus_tag="RH057_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HDV3130132.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLVGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(2178..3272)
                     /gene="yaiW"
                     /locus_tag="RH057_003830"
     CDS             complement(2178..3272)
                     /gene="yaiW"
                     /locus_tag="RH057_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HDV3130133.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVCSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(3285..4505)
                     /gene="sbmA"
                     /locus_tag="RH057_003831"
     CDS             complement(3285..4505)
                     /gene="sbmA"
                     /locus_tag="RH057_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HDV3130134.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIVGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            4857..6014
                     /gene="ampH"
                     /locus_tag="RH057_003832"
     CDS             4857..6014
                     /gene="ampH"
                     /locus_tag="RH057_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HDV3130135.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITRPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPGIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(6015..6638)
                     /gene="iprA"
                     /locus_tag="RH057_003833"
     CDS             complement(6015..6638)
                     /gene="iprA"
                     /locus_tag="RH057_003833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogen peroxide resistance inhibitor IprA"
                     /protein_id="HDV3130136.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNSEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     ITLIEQNQLWRDAFYWLAWQNRILELRDVQLIGHNSYEQIRATLLSMIDWNEELRSRI
                     GVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAINRLPSEY"
     gene            complement(6726..9728)
                     /gene="ehaB"
                     /locus_tag="RH057_003834"
     CDS             complement(6726..9728)
                     /gene="ehaB"
                     /locus_tag="RH057_003834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin EhaB"
                     /protein_id="HDV3130137.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYAADSYYNGDIYPVINNATVNGVISTYYLDDGISTNTNAN
                     SLTIKNSTIHGMIYSECMTTDCADRADDYYHDRLALTVDNSTIDDNYEHYTYNGTYNN
                     AADTHVVDVYNIGTAITLDQEVDLSITNNSHVAGITLTQGYEWEDIDDNTVSTGVNSS
                     EVFNNTITVKDSTVTSGSWTDEGTTGWFGNTGNASDYSGKSNFVTVDTDGDGVADSTI
                     ASWDDVALAVVAHPNTDNAMQTTADFSNSTLMGDVIFSSNFDENFFPRGADSYRDADG
                     EVDTNGWDGTDRLDLTLNNGSKWVGAAQSVHQTGSIDVDGDGKGDIATYGVGTEATAT
                     LIDIEDNSLWPLSTVGVENDDTSYSEFDHITGNQVYQSGLFNVTLNTGSQWDTTKTSL
                     IDTLSINSGSTVNVADSTLISDSISLTGLSALNINEDGHVATDSLTVDNSTVTISDEV
                     SAGWAVGDAALYANNIKVTNDGILDVGNTAANALQVDTLNLTSTTDTSGNIHAGVFNI
                     ESNRFVLDADLTNDRTNDTTKSNYGYGLIAMNSDGHLTINGNGDNDNTASIEAGQNEV
                     DNNGDHVAAATGNYKVRIDNATGAGSIADYNGNELIYVNDKNSNATFSAANKADLGAY
                     TYQAEQRGNTVVLQQMELTDYANMALSIPSANTNIWNLEQDTVGTRLTNSRHGLADNG
                     GAWVSYFGGNFNGDNGTINYDQDVNGIMVGVDTKIDGNNAKWIVGAAAGFAKGDMNDR
                     SGQVDQDSQTAYIYSSAHFANNVFVDGSLSYSHFNNDLSATMSNGTYVDGSTNSDAWG
                     FGLKAGYDFKLGDAGYVTPYGSISGLFQSGDDYQLSNDMKVDGQSYDSMRYELGVDAG
                     YTFTYSEDQALTPYFKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNF
                     SAYTDANYLGGGDVDQDWSANVGVKYTW"
BASE COUNT         2567 a         2464 c         2366 g         2568 t
ORIGIN      
        1 gaaaaaaact caccgcatgg ccatttaagc caggaataaa ttagcttccc gttcatcatt
       61 tttgtgatct tgcgcacatc tttgagataa cgcactttct tcaccagtac gttgcacgct
      121 ttgtcagcag aagattgcaa caatctttca gaaggccgcg aatcccccgt tttgtcgtgg
      181 taatgtatgc actttgtgtt agacgggata ggtttttaag atggaaaaac tgcgggtagg
      241 aatcgttttt ggtggtaaat cagcggaaca tgaagtgtct ctgcaatcgg caaaaaacat
      301 tgtcgatgcc attgataaaa gtcgcttcga cgttgtgctg ctgggcattg ataaacaagg
      361 gcaatggcac gtcagcgatg ccagcaatta tctgctaaat gcagacgatc ctgcccatat
      421 tgcgttgcgc ccttcggcga ccagccttgc gcaggtgcca ggtaaacatg agcatcagct
      481 tatcgacgcg caaaacggtc agccgttgcc gacggtggat gtcattttcc cgattgtcca
      541 cggtacgctg ggcgaagatg gttccttgca gggaatgctg cgggtcgcca atttaccgtt
      601 tgtaggttct gatgttctgg cttcagcagc ctgtatggat aaagatgtca ccaaacgtct
      661 gctacgcgat gccgggctga acattgcgcc atttattacc ctgacgcgcg ctaatcgtca
      721 caacatcagt tttgccgaag tggagtctaa actggggtta ccactgtttg taaaaccggc
      781 taatcagggc tcttctgttg gtgtcagcaa agtaaccagt gaagaacagt acgcaattgc
      841 cgtcgatctg gcgttcgagt tcgatcataa agtgatcgtt gagcaaggga tcaaaggtcg
      901 tgagatcgaa tgcgcagttc tgggcaacga caatccgcaa gccagcacct gtggcgagat
      961 cgtactcacc agcgatttct atgcctacga caccaagtac attgacgaag atggcgcgaa
     1021 agtggtagtt ccggcagcca ttgcaccaga gatcaacgat aagatccggg cgattgccgt
     1081 tcaggcttat cagacgttgg gatgcgcagg catggcgcgt gtagacgtgt ttttaacccc
     1141 agagaacgaa gtggtgatca acgagatcaa cacactgcct ggcttcacca acatcagtat
     1201 gtatccgaag ctgtggcaag ccagcggtct gggttacacc gatctgatca cacgtttgat
     1261 tgagctggcg ctggagcgtc acgctgcgga taacgcattg aaaaccacaa tgtaatattt
     1321 taacgcccga taatattctt attgggcgtt ttcagtttct tcatcgcgac gacgaataat
     1381 caaccccgcc agccagaaac taatcaccca cgtcaccaga ccgacggcat aggtctgcca
     1441 accttttgct tcaaatccca gtagccccac cacgccatta agaatgaata taaggccaat
     1501 ggcgaacgca tagtagtgcc agtcacggcg gatttttaca ggcagattca tggcagctcc
     1561 gttaacgaaa aacaacatcc ttgcatattc tgtaacaacc gtctgtggtg tagacaacat
     1621 ttttgtaatt gttaataaat ttaacttaag gttgccattt tgtgactcac aacgaagatt
     1681 cacgcttcgg gaatattccc aacatcaatt taccatgatt ctgatattga caatctaaat
     1741 gacctgtcag acttgccata agccgctggt atcacaccag aagagaagca gatgacttcc
     1801 ggaggttttt atggctgatt tcaccctgtc aaaatcgctg tttagcggaa aatatcgcaa
     1861 tgcctcttca acgcctggca acattgccta tgcgttgttt gtgctgtttt gcttttgggc
     1921 tggggcgcaa ttgctgaacc tgttagtgca tgcgcccggc gtctatgagc gtttaatgca
     1981 ggtccaggaa acaggtcgcc cacgggtgga aattggttta ggtgtcggca ccattttcgg
     2041 gctgatcccg tttttagtag gctgcctcat ttttgcagtg gtggcgctat ggctgcactg
     2101 gcgacatcgc cgccagtaag atttacgttg ccagatacgg cctaaaacgc cttatctggc
     2161 atgacattcc tgtttgatca ctgtttcata caacgcgccc gccgttcgtc tacgcgcttc
     2221 gcaaaccagg ccgtagtcag gttgcgggtg attttcgggc tttccagttg aatgccaggc
     2281 aacatttcgc ggggtagtga tttacccgtt ttcgcttcgg caagttgata aactttcttg
     2341 tacagcgccg tctcctcaaa agagaagctg tcacctttct ctaactgacg gcgaatttcg
     2401 ctgtcgctca tccccagttt tcccgccagt ttgcgcgttg ccagttcggt tttcccgggt
     2461 tctttactgt cgtaacgaat taaatcaccg tccagtgcca gcttcacgcc gctggcctta
     2521 ctgactgcgt tctgaaacgc ggcattacgg ctggcgtacc agccagcgtt aaaatcagca
     2581 aagcggtata tcggtgcgct atagctggcg ggataattca gtaaatggta agtaccaaac
     2641 cacaacccgc cgcggcgact gaagacttcc tgacgaactg taccgtccat tttccacgga
     2701 taccctttgg tatgctgttc ggcaaaagca atgctgacct gcatcggccc accggtgcgc
     2761 accggattaa gcgaaccaaa cagcgtctgc cccattggta ccatgctgat cagatcgtca
     2821 aaaatcgcgc tcaattgctt ttctgtacgc accgaatcta accgttcgct ataacttttg
     2881 ccgtttggcg atttgatttt tagcgcggta tgaaccagaa aagcaggaat gtgcatccgt
     2941 tcagcacgac ggtcaatctc ttgccaggcg atcttgctta agcccggaac ggccggatct
     3001 gcctgataat ttgactcctg ctgcgccacc gccagcaccg agcagacgtt ttccagcgtc
     3061 ggtgccagcc cctgactttc aaaggtggtg gcgagatctt tcgcccaggc atcgcggtct
     3121 ttcacgctcg caggcatctt ctgccgcacg acgctcgcta catctatcgc tttttcgccc
     3181 tttttcaacg gttgcggagc ctgactacta caccccgcta aaaccagaac cgccaacaat
     3241 gacagcgaag atagatgatt gacgcgcgac attacttctc ctttttagct caaggtatgg
     3301 gttacttcct gaattttgtc accatccagc tcatgttcaa agctgcgcag acgtttgtag
     3361 atagacatca actcaaccag tgtggtccat gagttaatca ggtactggaa agcaccgcga
     3421 acctgaccaa aaacgttggt aatctgcgtc atcaggccga gcgtaatcgt accggcaaca
     3481 attgacggaa acagcaagaa caaaccgaaa acgttatcga cctgcaaata gagaatgcgg
     3541 gcgatgttga aatacatata gtgaaaatag aggcgaaaat agtttttccg tacggcgcta
     3601 aacagctcgc gtaccgtagg cggcgtcgcg cgcgtggcat cgtcttcacc ataaaccagc
     3661 tctttacggt aggcggcctc tactcgctgg tttttaaact ccagccccgg cagtttgatc
     3721 cctaccactg ccagcaatcc ggtccccatc agcgaccaga cgatagcggc aatcaccaga
     3781 ccatacggaa tgtgcccgac aattggcagc tccggcacat gtgcggagag cgttaccagc
     3841 accggcagga aggcgatcaa cgtcatgatg gcgttgataa aactgacgcc catattctcc
     3901 agcgttgaag caaaacgcat ggtgtcttcc tgcacacgct gtgcggcccc ttcgatatga
     3961 cgcagttgtt gccagttcgc catgtaatat tcgttcatcg ccgtacgcca gcggaacacg
     4021 tagtgactga caaagaagtt gttcagtaca ctgatcacca ccgcgatcag cgcaatcccc
     4081 agaaagacgc ccacttcgcg gtaaaattgt tcgatagtga ctttatgcgg cgaacttagc
     4141 gcggtttgaa tcagatcata gaacggtgca taccaggcgt tgacggcgac cccgacttcc
     4201 accaaaaacc aggtgacgaa gatgatcagt gcagtaccga gtattgacca gtattgccaa
     4261 cggtgcgggc tgtagataaa ccagaaaaat gcaaaaagtc ctacgcaaac aatgtagtaa
     4321 gcgtaaaaaa tcaggaaatc caacgaccag aaacgcgcgg cgctaatcgg gatctgcccg
     4381 gaagcgccgg tgatacgcgc cacccagtca cccccaccgg cttgccagaa gataacggca
     4441 atcaatgccc aaacaaaggc cgagagaaaa aacgttcccg gctttgggaa aaaagactta
     4501 aacatatgca ctcctgctaa cttcttatcg ttttaacaac tgctgtgtat taaccgcatg
     4561 accgctacaa agcgtcagcc aaatgaaacc gcccattgtg acccatagca aggcgcttag
     4621 ttcgccagcc tgaccataag aattgtttcg acggatttca caaaccaaag attgtttaca
     4681 taagcggatt aacacgcgca ggatcacaaa tgcaggcatt ggtgagcagg aaaaagatcg
     4741 tgtttacgcg ccgttacgtt tttaacacta ttattgcact aattcgccct ttgcaatcca
     4801 tcaatgagta gtataaatac gctcagttac cttcattcaa tctatggaca ccaccgttga
     4861 aacgtagtct gcttttttct gccgtgctgt gtgcggcgtc attgacctcc gtccatgcgg
     4921 cgcagccgat cactgaaccg gagtttgcct ctgatattgt cgatcgttat gccgatcata
     4981 ttttttacgg cagcggtgcc acggggatgg cgctggttgt tatcgacggt aaccagcgcg
     5041 tctttcgtag ttatggcgaa acgcgacctg gtaataacgt tcgcccgcag ctggattcgg
     5101 tcgtgcgtat tgcttccctc accaaactaa tgaccagtga aatgctggtg aaattgctcg
     5161 accaggggac cgtaaagtta aacgatccgc taagtaaata tgctccgcca ggcgcacgcg
     5221 tgccgaccta caatgggacg cccattaccc tagtgaatct ggcaacccat accagcgccc
     5281 tgccccgtga acagcccggt ggcgcggcac atcgtccggt gtttgtctgg ccaacgcgcg
     5341 aacaacgctg gaaatacctt tctacggcga agctaaaagc cgcgccaggc agtcaggcgg
     5401 cgtactctaa ccttgcgttc gacctgctgg cagatgcact ggcgaatgcg tccggcaagc
     5461 cttataccca gttgtttgaa gagcagatta cccgtccatt ggggatgaaa gacaccacct
     5521 acaccccctc accggatcag tgccgccgtt taatggttgc tgagcgcggt gccagtccgt
     5581 gcaataacac gctggcggca attggcagcg gcggcgtcta ttccacgcct ggcgatatga
     5641 tgcgctggat gcagcagtat ttgtcatctg atttttatca gcgcagtaac caggcagatc
     5701 gcatgcagac gttgatttat cagcgcgcgc agtttaccaa agtgattggc atggatgtcc
     5761 ccggcaaagc ggatgcgctt ggtttaggct gggtatatat ggccccgaaa gagggtcgtc
     5821 cggggattat tcagaagaca ggcggtggtg gcggtttcat tacctatatg gcgatgatcc
     5881 cgcagaagaa tattggcgcg tttgtggtgg tcactcgttc gccgttgacg cgctttaaaa
     5941 atatgagcga tggcatcaat gacctggtga ccgagctaag cgggaataaa ccgttggtta
     6001 tccccgcatc ctgattaata ctctgaaggc aaacggttga tagcgaccag tttgccttta
     6061 ttcatttcga tatagccgcc tttacgcaaa gcagcgagaa cttctgcgac gacagaacgc
     6121 gatatgcgtg tacgttgatg gatataattc atcacaccaa tacgtgatcg caattcttca
     6181 ttccagtcaa tcattgataa taatgttgcg cggatttgtt cgtaggaatt atgcccaatg
     6241 agctgcacgt cgcgtaattc cagaattcta ttttgccagg ctaaccagta aaaagcgtct
     6301 cgccagagtt gattttgttc aataagcgta atggtttgtt tggctggtag atgatatccc
     6361 gtacaatttc cttctgatat taatttgtat ggtatatcgt ttttcattaa accatcagcc
     6421 agccccataa tataaggagc ctgggtaata ccgataagta cgttttcttc tctacgcaga
     6481 gagataacgc cctctaaaat aacgaaagta tcttcgctat tgacatcact ggaaaatata
     6541 acttgctttt cattattaaa ctcgaagcgc gtaccgtatc tggacaaaca tttatcgagc
     6601 ttaccaaatt cctgaagagg tttaactaca gataacattt gcgcgtcctt tgcagtaatg
     6661 cccgtcaaat ccttgacggg cattatttag attaaattac cagaatttct tcggagtgaa
     6721 gaatattacc aggtatattt aacacccacg ttcgcggacc agtcttgatc tacgtcacca
     6781 ccaccgaggt agttagcatc ggtataggcg ctgaagttct tggtgaagct gaactgagta
     6841 cccagaccaa cacgtaccgc agacccttca gtaccgttat cgatggaatc accgttcaca
     6901 tcgttatcgt tgttagagtc gtcgtagacg taagccagtt tgaagtacgg agtcagagcc
     6961 tggtcttcgc tgtaggtgaa ggtataacct gcatctacac ccagttcata acgcatgctg
     7021 tcgtaagact gaccgtcaac tttcatgtcg ttgctcagct ggtagtcatc accagactgg
     7081 aacagaccag aaatgctgcc gtaaggagtc acgtaaccag catcacccag tttgaagtcg
     7141 taaccggctt tcaaaccgaa gccccaagcg tcggagttgg tgctaccgtc aacgtaagta
     7201 ccgttgctca tggttgcaga caagtcgttg ttgaagtgag agtaactcaa gctaccatca
     7261 acaaagacgt tgttcgcgaa gtgagcagaa gagtagatgt aggcagtctg gctgtcttga
     7321 tccacctgac cagaacggtc attcatgtca cctttagcga agcctgcagc cgcaccgacg
     7381 atccacttag cgttgttacc gtcaattttg gtatcaacac cgaccatgat gccgttaaca
     7441 tcctgatcat agttgatggt gccgttgtcg ccgttgaagt taccaccgaa gtagcttacc
     7501 catgcgccgc cgttatcagc caggccatga cgagagttgg tcagacgagt accaacggtg
     7561 tcttgttcca ggttccagat attggtgttc gcagacggga tgctcagcgc catgttagcg
     7621 tagtcggtca gctccatctg ttgcagaaca acggtgttac cgcgctgttc agcctgatag
     7681 gtgtatgcac ccaggtcagc tttgttagca gcagagaagg tcgcgttgct gtttttgtcg
     7741 ttgacgtaga tcagctcgtt gccgttgtag tcagcgatag aaccagcacc agttgcgttg
     7801 tcgatacgaa ctttgtagtt accggttgca gctgcaacat ggtcaccgtt gttatcaact
     7861 tcgttctgac cagcttcgat agaagcagtg ttgtcgttat cgccgttacc gttaatggtc
     7921 aggtgaccat cagagttcat tgcgattaag ccataaccgt agtttgactt ggtagtatcg
     7981 ttggtacggt cgttggtcag gtctgcatca agtacgaagc ggttgctttc gatgttgaac
     8041 acaccagcgt gaatgttacc actggtatca gtagtgctgg tcaggttcag agtatcaacc
     8101 tgcagagcat tcgccgcagt gttacctaca tccagaatac cgtcgttagt cactttgatg
     8161 ttgttggcat acagagcagc atcacctaca gcccaaccag cagaaacttc atcagaaatg
     8221 gttacggtac tgttgtcgac agtcagtgaa tcagttgcaa catgaccatc ttcgttgatg
     8281 ttcagcgcag aaagacctgt cagagagata gagtcagaaa tcagcgttga atcagcaaca
     8341 ttaacagttg aaccactgtt gatgctcaga gtatcaatca gagaagtttt ggtggtatcc
     8401 cactgtgaac cggtattcag ggtcacattg aacagaccgc tctggtaaac ctgattacca
     8461 gtaatatgat cgaattcact gtaacttgta tcatcgtttt caacaccaac ggttgataac
     8521 ggccacaggc tattatcctc aatatcaatc agagttgcag ttgcctcggt gccaacaccg
     8581 tatgtagcga tgtcaccttt accatctcca tcaacatcaa tagaaccagt ttgatgaaca
     8641 gattgtgctg cccctaccca tttactaccg ttatttaatg tcagatccag gcgatctgtt
     8701 ccatcccaac cgttggtgtc tacttcacca tcagcatcgc gataactatc tgcaccacgt
     8761 gggaagaagt tttcatcaaa gttgctagag aagattacat cgcccatcaa agtagagtta
     8821 ctaaagtcag cggtggtctg catagcatta tcagtattcg gatgtgcaac tacagctaat
     8881 gcaacatcat cccagcttgc aatagtgcta tcagcaacac catctccgtc agtatctacg
     8941 gtgacaaagt tagatttacc actataatcg ctggcattac ctgtattacc gaaccaacca
     9001 gtagtacctt catcagtcca tgaaccagag gtcacggttg aatctttaac agtgatggtg
     9061 ttattaaaca cttcgctgct gtttacgcca gtgctgacag tgttgtcgtc aatatcttcc
     9121 cactcataac cctgagtcaa cgtaataccc gcaacgtgag agttattagt gatggacaga
     9181 tcaacttcct gatccagagt aatagccgta ccaatgttgt aaacgtctac aacatgagta
     9241 tcagcggcat tattgtaagt gccgttatat gtgtagtgtt cgtagttgtc gtcgatcgtt
     9301 gaattatcaa cagtcagcgc cagacggtca tggtagtaat catccgcacg atcagcacaa
     9361 tcagttgtca tgcactcgga ataaatcata ccgtgaatag tgctgttttt gattgtcaga
     9421 ctattggcgt tggtattggt agaaataccg tcgtccagat agtaggtaga aatcacgccg
     9481 ttaacggtag cgttattaat gaccgggtag atatcgccgt tgtaatagct atcagcggca
     9541 tagttgttcc agcctacata accatcatag taaacgtcat cagcataggt ggcgtcgttg
     9601 tagtgatgga aagtattgta agtttgacca gaaatatcgt tggtcgctgc attagcctga
     9661 gaggtgatag ccagagtgca agccaatgct aattgtgata ctacaagttt ctttttccag
     9721 gagtgcattt gtcatccctc ctcagggacg taataagttg atccatcaat tatcaatgca
     9781 tagaaaacat attatgtttc gcgctaacga ttatgctgcg ttacagacga atggttcaat
     9841 tattctttcc cctaagtccg aatgtggaca tatataattt atggagaaaa acacgcatca
     9901 aattgataaa aggacgatat ttcaacatgt tgcagataat gttatttttg atacatacgc
     9961 gttat
//
LOCUS       DAPHDW010000156         9964 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.159, whole genome shotgun sequence.
ACCESSION   DAPHDW010000156 DAPHDW010000000
VERSION     DAPHDW010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9964
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.159"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            301..1278
                     /gene="csiD"
                     /locus_tag="RH057_003835"
     CDS             301..1278
                     /gene="csiD"
                     /locus_tag="RH057_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HDV3130138.1"
                     /translation="MNALTAVHNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDEQNMQGGNSLLLHLDDWEHLDHYFRHPLARRPMRFAAPP
                     SKNVSKDVFHPVFDVDQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVP
                     VPVGKFLLINNLFWLHGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            1298..2566
                     /gene="lhgO"
                     /locus_tag="RH057_003836"
     CDS             1298..2566
                     /gene="lhgO"
                     /locus_tag="RH057_003836"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HDV3130139.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNDIRYDNCGKMLVATSDLEMERM
                     RALWERTAANGIEREWLNAEELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSGLSEHKSGVVIRTRQGGDYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILGSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            2589..4037
                     /gene="gabD"
                     /locus_tag="RH057_003837"
     CDS             2589..4037
                     /gene="gabD"
                     /locus_tag="RH057_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HDV3130140.1"
                     /translation="MKLNASNLFRQQALINGEWLDANNGEVIDVTNPANGDKLGSVPK
                     MGADETRAAIDAANRALPAWRALTAKERATILRNWFNLMMEHQDDLARLMTLEQGKPL
                     AEAKGEISYAASFIEWFAEEGKRIYGDTIPGHQADKRLIVIKQPIGVTAAITPWNFPA
                     AMITRKAGPALAAGCTMVLKPASQTPFSALALAELAIRAGIPAGVFNVVTGSAGAVGN
                     ELTSNPLVRKLSFTGSTEIGRQLMEQCAKDIKKVSLELGGNAPFIVFDDADLDKAVEG
                     ALASKFRNAGQTCVCANRLYVQDGVYDRFAEKLQQAVSKLHIGDGLDKGVTIGPLIDE
                     KAVAKVEEHIADALEKGARVVCGGKADERGGNFFQPTILVDVPANAKVSKEETFGPLA
                     PLFRFKDEADVIAQANDTEFGLAAYFYARDLSRVFRVGEALEYGIVGINTGIISNEVA
                     PFGGIKASGLGREGSKYGIEDYLEIKYMCIGL"
     gene            4051..5331
                     /gene="gabT"
                     /locus_tag="RH057_003838"
     CDS             4051..5331
                     /gene="gabT"
                     /locus_tag="RH057_003838"
                     /EC_number="2.6.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
                     /protein_id="HDV3130141.1"
                     /translation="MSSNKELMQRRSQAIPRGVGQIHPIFADRAENCRVWDVEGREYL
                     DFAGGIAVLNTGHLHPKVVAAVEAQLKKLSHTCFQVLAYEPYLELCEIMNQKVPGDFA
                     KKTLLVTTGSEAVENAVKIARAATKRSGTIAFSGAYHGRTHYTLALTGKVNPYSAGMG
                     LMPGHVYRALYPCPLHGISEDDAIASIHRIFKNDAAPEDIAAIVIEPVQGEGGFYAAS
                     PAFMQRLRALCDEHGIMLIADEVQSGAGRTGTLFAMEQMGVAPDLTTFAKSIAGGFPL
                     AGVTGRAEVMDAVAPGGLGGTYAGNPIACVAALEVLKVFEQENLLQKANDLGQKLKDG
                     LLAIAEKHPEIGDVRGLGAMIAIELFEDGDHSKPDAKLTAEIVARARDKGLILLSCGP
                     YYNVLRILVPLTIEDAQIRQGLEIISQCFAEAKQ"
     gene            5642..7042
                     /gene="gabP"
                     /locus_tag="RH057_003839"
     CDS             5642..7042
                     /gene="gabP"
                     /locus_tag="RH057_003839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HDV3130142.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAG
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDTPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLYPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            7063..7725
                     /gene="csiR"
                     /locus_tag="RH057_003840"
     CDS             7063..7725
                     /gene="csiR"
                     /locus_tag="RH057_003840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HDV3130143.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLR
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLARDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(7726..8175)
                     /gene="kbp"
                     /locus_tag="RH057_003841"
     CDS             complement(7726..8175)
                     /gene="kbp"
                     /locus_tag="RH057_003841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HDV3130144.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(8259..8417)
                     /locus_tag="RH057_003842"
     CDS             complement(8259..8417)
                     /locus_tag="RH057_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HDV3130145.1"
                     /translation="MGFWRIVITIILPPLGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
     gene            8600..8899
                     /locus_tag="RH057_003843"
     CDS             8600..8899
                     /locus_tag="RH057_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3130146.1"
                     /translation="MTELAQLQASAEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLIDSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            8909..9433
                     /gene="ygaP"
                     /locus_tag="RH057_003844"
     CDS             8909..9433
                     /gene="ygaP"
                     /locus_tag="RH057_003844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HDV3130147.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILIGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(9480..9884)
                     /gene="stpA"
                     /locus_tag="RH057_003845"
     CDS             complement(9480..9884)
                     /gene="stpA"
                     /locus_tag="RH057_003845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HDV3130148.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
BASE COUNT         2284 a         2560 c         2725 g         2395 t
ORIGIN      
        1 ataatatgtt tagtttacta acggtcattt tgcagtgaag ccatttactg ttttttatcg
       61 accagataat ctgttctcta atgttaactc cccctaacct gttgctttag ttattcattt
      121 cctgtctcac tttgccttaa taccctacgt taaatgttac taatttgttg cttttgatca
      181 caataagaaa acaatatgtc gcttttgtgc gcatttttca gaaatgtaga tatttttaga
      241 ttatggctac gaaatgagca tcgccatgtc accctacatc tcataagagg atcgcttctg
      301 atgaatgcac tgaccgccgt acacaataac gctgtcgatt caggccagga ctatagcgga
      361 ttcactctca tcccgtcggc gcaatccccg cgtctgctgg aactcacctt caccgaacag
      421 acgaccaaac agtttctcga acaggttgcc gaatggcccg tgcaggcgct ggagtacaaa
      481 tcgtttctgc gttttcgggt aggcaaaatt cttgacgatc tgtgtgcgaa tcagctgcaa
      541 ccattgctgt tgaagaccct gctaaaccgt gctgaaggtg cgctgttgat caatgcggtg
      601 ggtatcgatg atgtggcgca ggcagatgag atggtgaagc tggcaacggc ggtagcgcat
      661 ctgattggtc gctcaaattt cgatgcgatg agcggtcagt attacgcgcg tttcgtggtg
      721 aaaaatgtcg ataactcaga cagctatctg cgtcagccgc accgcgtgat ggaactgcac
      781 aacgacggca cgtatgtcga agagatcacc gattacgtgc tgatgatgaa aatcgatgag
      841 caaaacatgc agggtggaaa ttcgttgctg ctgcatctcg atgactggga acatctggac
      901 cactatttcc gccatccact ggcgcgtcgc ccgatgcgct ttgccgcgcc gccgagcaaa
      961 aacgtcagca aagatgtttt ccatccagtg ttcgacgttg atcaacaggg tcgcccggtg
     1021 atgcgctata tcgaccagtt cgtccagcca aaagattttg aagaaggcgt gtggttgagc
     1081 gagctttccg atgccattga aaccagcaaa ggcattcttt ctgtacccgt tcccgttggc
     1141 aaattcctgt tgataaacaa cctgttctgg ctgcacggtc gcgaccgctt tactccgcac
     1201 ccggatctgc gccgtgaact gatgcgtcag cgtggctatt tcgcttacgc cactcaccac
     1261 taccagacgc atcagtaagc gcaaaggaat tgagcggatg tatgattttg tgattattgg
     1321 cggcggcatc atcggcatgt cgaccgccat gcaactgatt gatgtttatc cggatgcccg
     1381 cattgcgttg ctggaaaaag agtccggccc ggcctgtcac cagacgggcc acaacagcgg
     1441 cgtgatccat gccggggtct attacacgcc cggcagcctg aaggcgcagt tttgcctggc
     1501 gggaaaccgc gccaccaaag ccttttgcga tcaaaacgac attcgctatg acaactgcgg
     1561 caagatgctg gtcgccacgt ccgatctcga aatggaacgg atgcgcgcgt tgtgggaacg
     1621 cacggcggcg aacggtatcg agcgcgagtg gttaaacgcc gaggaactgc gcgagcgcga
     1681 accgaatatc accgggctcg gcggcatttt tgtgccgtcc agcggcattg tcagctaccg
     1741 cgaagtaacg gcggcgatgg caaaaatttt ccaggccaga ggcggcgaga ttatctataa
     1801 cgccgaagtc agcggcctta gtgagcataa aagcggcgtg gtgatacgta cccgtcaggg
     1861 cggcgactat gaagcatcga cgctgattag ctgttccggg ctgatggctg accggctggt
     1921 gaaaatgctc ggcctcgaac cgggctttat catctgcccg ttccgcggcg agtatttccg
     1981 cctggcgccg gagcataacc agattgttaa ccatctgatt taccccattc ccgacccggc
     2041 aatgccgttt ttgggcgtgc atctcacccg aatgatcgat ggcagcgtca ccgtcgggcc
     2101 aaacgcggtg ctggctttca aacgcgaagg ctatcgcaag cgcgacttct catttagcga
     2161 cacgctggag attttgggct cgtcggggat tcgccgggtg ctgcaaaacc atctacgctc
     2221 aggactgggc gagatgaaaa actcgctgtg caaaagcggc tatctgcgcc tggtgcaaaa
     2281 gtattgtccc cggctttcgt taagcgatct ccagccctgg cccgccggtg tgcgggcgca
     2341 ggcagtatcg ccggacggca agctgattga cgattttctg tttgtcacca ccccacgcac
     2401 gatccacacc tgcaatgcgc cctccccggc agcgacatca gcaattccta ttggtgcgca
     2461 tattgtcagc aaggtacaaa cgctgttggc aagccagagt aaccccggac gcacgctgcg
     2521 tgcggcacgt agtgtggatg ccttacacgc cgcatttaat caataacctt tgaaaacagg
     2581 acgtaacaat gaaacttaac gccagtaact tattccgcca gcaggcattg attaacgggg
     2641 aatggctgga cgccaacaat ggcgaggtca tcgacgtcac caatccggcg aacggcgaca
     2701 agctgggtag cgtacccaaa atgggcgctg atgaaacccg cgcggctatc gacgccgcca
     2761 accgcgccct gcccgcctgg cgcgcgctca ccgccaaaga acgcgccacc attctgcgca
     2821 actggttcaa tttgatgatg gagcatcagg acgatttagc gcgcctgatg accctcgaac
     2881 agggtaaacc gctggctgaa gcgaaaggcg aaatcagcta cgccgcctcc tttattgagt
     2941 ggtttgctga agaaggcaaa cgtatttatg gcgacaccat tcccggtcat caggccgata
     3001 aacgcctgat tgttatcaag cagccgattg gcgtcactgc tgctatcacg ccgtggaact
     3061 ttccggcggc gatgattacc cgtaaagccg gtccggcgct ggcggcaggt tgcaccatgg
     3121 tgctgaagcc cgccagtcag acgccgttct ctgcgctggc gctggcagag ctggcgatcc
     3181 gcgcgggcat tccggctggc gtgtttaacg tggtcaccgg ttcggcgggc gcggtgggta
     3241 atgaactgac cagcaacccg ctggtacgca aactgtcgtt taccggctcg accgaaattg
     3301 gccgccagtt aatggaacag tgcgcgaaag acatcaaaaa agtgtcgctg gagctgggcg
     3361 gcaacgcgcc gtttatcgtc tttgacgatg ccgatctcga taaagccgtg gaaggcgcgc
     3421 tggcctcgaa attccgcaac gccgggcaaa cctgcgtctg cgccaaccgt ttatacgtgc
     3481 aggacggcgt gtatgaccgc tttgccgaaa aactacagca ggcggtgagc aaactgcaca
     3541 tcggcgacgg gctggataaa ggcgtcacca tcgggccgct gatcgatgaa aaagcggtag
     3601 caaaagtgga agagcatatt gccgatgcgc tggagaaagg tgcgcgtgtg gtttgcggcg
     3661 gtaaagcgga tgaacgcggc ggcaacttct tccagccgac cattctggtg gacgtgccgg
     3721 ccaacgccaa agtgtcgaaa gaagagacgt tcggccccct cgccccgctg ttccgcttta
     3781 aagatgaagc cgatgtgatt gcgcaagcca atgacaccga gtttggcctt gccgcctatt
     3841 tctacgcccg tgatttaagc cgcgtcttcc gcgtgggcga agcgctggag tacggcatcg
     3901 tcggcatcaa taccggcatt atttccaatg aagtggcccc gttcggcggc atcaaagcct
     3961 cgggtctggg tcgtgaaggt tcgaagtatg gcatcgaaga ttacttagaa atcaaatata
     4021 tgtgcatcgg tctttaactg gagaacgcga atgagcagca ataaagagtt aatgcagcgc
     4081 cgcagtcagg cgattccccg tggcgttggg caaattcacc cgattttcgc tgaccgcgcg
     4141 gaaaactgcc gggtgtggga cgttgaaggc cgtgagtatc ttgatttcgc gggcgggatt
     4201 gcggtgctca ataccgggca cctgcatccg aaggtggtgg ccgcggtgga agcgcagttg
     4261 aaaaaactgt cgcacacctg cttccaggtg ctggcttacg agccgtatct ggagctgtgc
     4321 gagattatga atcagaaggt gccgggcgat ttcgccaaga aaacgctgct ggttacgacc
     4381 ggttccgaag cggtggaaaa cgcggtaaaa atcgcccgcg ccgccaccaa acgtagcggc
     4441 accatcgctt ttagcggcgc gtatcacggg cgcacgcatt acacgctggc gctgaccggc
     4501 aaggtgaatc cgtactctgc gggcatgggg ctgatgccgg gtcatgttta tcgcgcgctt
     4561 tatccttgcc cgctgcacgg cataagcgag gatgacgcta tcgccagcat ccaccggatc
     4621 ttcaaaaatg atgccgcgcc ggaagatatc gccgccatcg tgattgagcc ggttcagggc
     4681 gaaggcggtt tctacgccgc gtcgccagcc tttatgcagc gtttacgcgc gctgtgtgac
     4741 gagcacggga tcatgctgat tgccgatgaa gtgcagagcg gcgcggggcg taccggcacg
     4801 ctgtttgcga tggagcagat gggcgttgcg ccggatctca ctacctttgc gaaatcgatc
     4861 gcgggcggct tcccgctggc gggcgtcacc gggcgcgcgg aagtgatgga tgccgtcgct
     4921 ccaggcggtc tgggcggcac ctatgccggt aacccgattg cctgcgtggc ggcgctggaa
     4981 gtgttgaagg tgtttgagca ggaaaatctg ctgcaaaaag ccaacgatct ggggcagaag
     5041 ctaaaagacg gattgttggc gatagccgaa aaacacccgg agatcggcga cgtacgcggg
     5101 ctgggagcga tgattgccat cgagctgttt gaagatggcg atcacagcaa accggacgca
     5161 aaactcaccg ccgagatcgt ggcacgcgcc cgcgataagg gcctgattct tctctcctgc
     5221 ggcccgtatt acaacgtgct gcgcatcctt gtaccgctca ccattgaaga cgctcagatc
     5281 cgtcagggtc tggagatcat cagccagtgt tttgctgagg cgaagcagta gctatgtccg
     5341 tttgtaagag ttaaacgttg ctaagggaat gataacgatg cagtaaaaaa taataacgat
     5401 gaggcgatcg acaaagttaa aaacgactgc ggactgtccc ctcgctcctc tggggagagg
     5461 gttagggtga ggggtaatca cgcggctaca ccctcacccc ggccctctcc ctaagggaga
     5521 gggagaaaac tctcccgctt tcgtttttaa gattttgttg accgtctcaa ttaaacgcgt
     5581 gacccgacgc gggaaatgtc ggggcgctct cccaagtgac acactttcga gaggattcag
     5641 gatggggcaa tcatcgcaac cacatgagtt aggcggcggg ctgaagtcac gccacgtcac
     5701 catgttgtct attgccggtg ttatcggcgc aagtctgttt gtcggttcca gcgtcgccat
     5761 cgccgaagcg ggcccggcgg tattactggc ctatctgttc gccggattac tggtggttat
     5821 gattatgcga atgctggcgg aaatggcggt tgccacgccc gataccggtt cgttttccac
     5881 ctatgccgat aaagccattg gccgctgggc gggttatacc atcggctggc tgtactggtg
     5941 gttttgggta ctggttatcc cgctggaagc caacatcgcc gctatgatcc tgcactcgtg
     6001 ggttccaggc attcccatct ggttattttc cctcgtcatt accctcgcct taactggcag
     6061 taatttatta agcgttaaaa actacggcga atttgagttc tggctggcgc tgtgcaaagt
     6121 catcgctatc ctggccttta ttttccttgg tgcagtcgca attagcggtt tttaccctta
     6181 tgccgaagtg agcgggatct caagattgtg ggatagcggc ggctttatgc ccaacggctt
     6241 cggtgcggta ttaagcgcga tgttgatcac catgttctcg tttatgggcg cagaaattgt
     6301 caccattgcc gccgcggaat ccgacacgcc ggaaaaacat attgtccgcg ctaccaactc
     6361 ggttatctgg cgtatttcta tcttctattt gtgctctatt tttgtcgtag tggcgttaat
     6421 accgtggaat atgccggggc tgaaagccgt tggttcttat cgctcggttc tggaattgct
     6481 caatattccc catgcgaaat taatcatgga ctgcgtgata ttactttccg taaccagctg
     6541 tctgaactcg gcgctgtata ccgcgtcaag gatgctctac tccttaagcc gtcgcggtga
     6601 tgcgcccgcg gtaatgggca aaatcaaccg cagtaaaacc ccgtatgtgg cggtgttact
     6661 ctccaccgga gcggcatttt taacggtggt ggtgaactat tacgcacctg cgaaagtgtt
     6721 taaattcctg atagacagct ccggtgctat cgccctgctg gtttatttag tcatcgccgt
     6781 ttcacagttg cggatgcgta aaattctgcg agcagaagga agcgaaattc gcttgcgcat
     6841 gtggctttac ccgtggctca cctggctggt aataggcttt attacctttg tgttggtggt
     6901 gatgttattc cgcccggcgc aacagttaga agtgatctct accggcttat tagcgatagg
     6961 gattatctgt accgtgccga ttatggcgcg ctggaaaaag ctggtattgt ggcaaaaaac
     7021 acccgttcat aatacgcgct gataatgatc aggagtcaca ccatgaccat tacgtctctg
     7081 gatggctatc gctggctgaa gaacgatatt attcgcggta attttcaacc ggatgaaaaa
     7141 ttacgaatga gtttgctgac atcgcgttat gcacttggcg ttgggccgtt acgggaagct
     7201 ctttcgcaac tggtggcgga acggctggtc acggtggtga atcaaaaagg gtatcgggtg
     7261 gcgtctatgt cagagcagga gctgctcgat attttcgacg cccgcgccaa tatggaagcg
     7321 atgctggtca gtctggcgat tgcccgcggt ggcgatgaat gggaggcaga cgttctcgca
     7381 aaagcgcatc tgctgagtaa gctggaggcc tgtgacgcca gcgagaaaat gcttgatgag
     7441 tgggatctgc gtcatcaggc gtttcataca gcaattgtgg cgggctgtgg ttctcactat
     7501 ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc gctatcgatt tatctggctg
     7561 cggcgaacgg tgctttcggt agaaatgctg gaggataaac acgatcagca ccagaccctg
     7621 actgcggcgg tactggcgcg agataccgcg cgcgccagtg agttaatgcg ccagcattta
     7681 ctgacgccaa ttcccattat ccagcaggcg atggctggca attaactact cttccggaat
     7741 acgcaacact tgccccggat aaattttatc cgggcttttt agcatcggtt tattcgcttc
     7801 gaagatttta ttgtacagat tagcgttacc gtagacctgt ttggaaatgg cactcagagt
     7861 gtcgccagac ttaacggtat aaaactggct ggcagtggct ggtgtcgccg ttttcacctg
     7921 atcatcaaca ctggcaatac cggaaatatt ccccaccgca acaaggattt tctccttcgc
     7981 ctcctgactc aggccgtcac cagtgaccgt cgctttgccg tcggcaattt gaatgttcac
     8041 tttatcggca tcaggtatac cggttttgct cagatgctcc tgcaccttct tcgcctgatc
     8101 gtctttatcg tgctgacctg taaccgcgtc ccagagtttt tctccggcat ctttcacaaa
     8161 attgaacaga cccatagcta cctcattcgt taacggaaac actcagaaag tgtagcagag
     8221 gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt aatcgcgggt ttgcacccag
     8281 aacgcgtgaa tcaaaccggg gatataccca agcagtgtca gcagaatatt aataatgaac
     8341 gcccaaccga accctttacc gagcagcacg ccgagaggcg gcagaatgat ggtgatgacg
     8401 attctccaga aacccatatg tactccctat aagaaaatta ctcattgttt aaaaagagat
     8461 tttatctctt aagcgtagta attttagcgg agcctgccag tttcagctta cgcttttact
     8521 ctcatttacg ctgctgatgc tggtatatgt tgcattttta gtttagagtt tacttaattt
     8581 agaaaatact taaataaata tgactgaact cgcgcaatta caggccagtg ccgaacaggc
     8641 agcggcctta ttgaaagcaa tgagccatcc taaacgattg ctgattctgt gcatgcttag
     8701 cggttccccc ggcaccagcg cgggagagct gacgcgcatt accggactga gtgcctctgc
     8761 gacatcacag catctcgctc gtatgcggga cgaagggctt atcgacagcc aacgggatgc
     8821 ccaacgcatt ctatattcca ttaaaaatga ggcggtaaat gccattatcg ccaccctgaa
     8881 aaatgtctat tgtccgtaag gagtcactat ggctttgaca accatttcgc cgcatgatgc
     8941 acaagaatta atcgcacgcg gcgcaaagtt aatcgatatt cgtgatgctg atgaatatct
     9001 tcgtgaacat attcctgaag cagatctggc tccattatcc gtgctggaac agtcaggtct
     9061 tccacctaaa ttacgtcgcg agcaaattat attccactgc caggcaggta aacgcaccag
     9121 taataacgcc gataaattag cagcgattgc cgcccccgca gaaatatttt tactcgaaga
     9181 tgggattgat ggctggaaaa gagctggatt gccagtagcg gtaaataaat ctcaaccctt
     9241 gccgttaatg cgccaggtgc agatcgctgc gggtggttta atattaatcg gcgttgtact
     9301 gggttatacc gtaaatagcg gtttcttctt attaagtggc tttgttggtg ccgggttact
     9361 gtttgcagga atcagcggtt tttgtggaat ggcaaggttg ttagataaga tgccgtggaa
     9421 ccaacgagct tgagaagcga cgccggacgc gccctagcag cgacatccgg cctcagtaat
     9481 tagatcagga aatcgtcgag agatttacct tctgccagcg cctgagcaat tggcttcggt
     9541 gtacggccct gaccggtcca ggttttagtt tcaccgttaa catcggtgaa tttatatttc
     9601 gccggacgcg gctggcgttt tttaccagcg cgtggtgcag cagcagagct attacccaat
     9661 aactcttccg ggttaattcc gtcagccttc atcagctcca gccaggtgct aattttttcc
     9721 tgacgctcag ccagttcacg ctgctgctgt tcttcttctt cacgtctttc tttagtgaca
     9781 accctgaatt tttcgagcat ttcttcaaga acgtcaatgg agaattcgcg agccatcgca
     9841 cggagggtgc gaatgttatt taaactttgt aacattacgg acataataaa gaaaaccttt
     9901 taacgccaaa acaaaaaagt atttcgtcgc gtagtgtaag cgatcactta ttatatttcc
     9961 acaa
//
LOCUS       DAPHDW010000157         9864 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.160, whole genome shotgun sequence.
ACCESSION   DAPHDW010000157 DAPHDW010000000
VERSION     DAPHDW010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9864
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.160"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(438..1685)
                     /locus_tag="RH057_003846"
     CDS             complement(438..1685)
                     /locus_tag="RH057_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide MFS transporter"
                     /protein_id="HDV3130149.1"
                     /translation="MALNIPFRNAYYRFASSYSFLFFISWSLWWSLYAIWLKGHLGLT
                     GTELGTLYSVNQFTSILFMMFYGIVQDKLGLKKPLIWCMSFILVLTGPFMIYVYEPLL
                     QSNFSVGLIPGALFFGLGYLAGCGLLDSFTEKMARNFHFEYGTARAWGSFGYAIGAFF
                     AGIFFSISPHINFWLVSLFGAVFMMINMRFKDKDHQCVAADAGGVKKEDFIAVFKDRN
                     FWVFVIFIVGTWSFYNIFDQQLFPVFYAGLFESHDVGTRLYGYLNSFQVVLEALCMAI
                     IPFFVNRVGPKNALLIGVVIMALRILSCALFVNPWIISLVKLLHAIEVPLCVISVFKY
                     SVANFDKRLSSTIFLIGFQIASSLGIVLLSTPTGILFDHAGYQTVFFAISGIVCLMLL
                     FGIFFLSKKREQIVMETPVPSAI"
     gene            complement(1757..2671)
                     /locus_tag="RH057_003847"
     CDS             complement(1757..2671)
                     /locus_tag="RH057_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoimidazole riboside kinase"
                     /protein_id="HDV3130150.1"
                     /translation="MSAKVWVLGDAVVDLLPESDGRLLPCPGGAPANVAVGIARLGGT
                     SGFIGRVGDDPFGALMQRTLLTEGVDITYLKQDEWHRTSTVLVDLNDQGERSFTFMVR
                     PSADLFLETTDLPCWRHGEWLHLCSIALSAEPSRTSAFTAMTAIRHAGGFVSFDPNIR
                     EDLWQDEHLLRLCLRQALQLADVVKLSEEEWRLISGKTQNDRDICALAKEYEIAMLLV
                     TKGAEGVVVCYRGQVHHFAGMSVNCVDSTGAGDAFVAGLLTGLSSTGLSTDEREMRRI
                     IDLAQRCGALAVTAKGAMTALPCRQELE"
     gene            2887..4320
                     /gene="cscA"
                     /locus_tag="RH057_003848"
     CDS             2887..4320
                     /gene="cscA"
                     /locus_tag="RH057_003848"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HDV3130151.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALAPGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWTFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRTISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLVLWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(4328..5386)
                     /locus_tag="RH057_003849"
     CDS             complement(4328..5386)
                     /locus_tag="RH057_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3130152.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKPGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            5566..5784
                     /locus_tag="RH057_003850"
                     /pseudo
     CDS             5566..5784
                     /locus_tag="RH057_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            5802..7130
                     /gene="dsdA"
                     /locus_tag="RH057_003851"
     CDS             5802..7130
                     /gene="dsdA"
                     /locus_tag="RH057_003851"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HDV3130153.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPGTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFPETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASGFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNTTHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(7238..8776)
                     /gene="emrY"
                     /locus_tag="RH057_003852"
     CDS             complement(7238..8776)
                     /gene="emrY"
                     /locus_tag="RH057_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HDV3130154.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPGLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQETMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(8776..>9864)
                     /gene="emrK"
                     /locus_tag="RH057_003853"
     CDS             complement(8776..>9864)
                     /gene="emrK"
                     /locus_tag="RH057_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HDV3130155.1"
                     /translation="IAFSGAYAYWSMELEDMISTDDAYVTGNADPISAQVSGSVTVVN
                     HKDTNYVRQGDILVSLDKTDATIALNKAKNNLANIVRQTNKLYLQDKQYSAEVASARI
                     QYQQSLEDYNRRVPLAKQGVISKETLEHTKDTLISSKAALNAAIQAYKANKALIMNTP
                     LNRQPQVVEAADATKEAWLALKRTDIKSPVTGYIAQRSVQVGETVSPGQSLMAVVPAR
                     QMWVNANFKETQLTDVRIGQSVNIISDLYGENVVFHGRVTGINMGTGNAFSLLPAQNA
                     TGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSMTATIDTKNEDIAEMPELASTVTSMP
                     AYTSKALVIDTSPIEKEISNIISHNGQL"
BASE COUNT         2774 a         2404 c         2228 g         2458 t
ORIGIN      
        1 cccttcaaac gaaatctacc ttcaccccgt aaaagatggg tttggcagca cacttgcctt
       61 atatctactc atttttactg caacaggttg aaatctcagc actgtcagaa agcgctgatg
      121 actaaacagc cctgagccgg gcgatgtaac catcacacag aatcctgata gcgaaatatg
      181 gcgtgactcg atacttcact ccgcaatgca ttccttgatg aattcgcagg accgtgatac
      241 acgggacagg tcactgaatg acgacaatgt cctggaaatc agcgaaccgc gcatctgaag
      301 tacatttgag cgactgtacc agaacatgaa tgaggcgttt ggattaggcg attattagca
      361 gggctaagca ttttactatt attattttcc ggttgaggga tatagagcta tcgacaacaa
      421 ccggaaaaag tttacgtcta tattgctgaa ggtacaggcg tttccataac tatttgctcg
      481 cgttttttac tcaagaagaa aatgccaaat agcaacatca ggcagacaat acccgaaatt
      541 gcgaagaaaa ctgtctggta gcctgcgtgg tcaaagagta tcccagtcgg cgttgaaagc
      601 agcacaatcc caagcgaact ggcaatttga aaaccaatca gaaagatcgt cgacgacagg
      661 cgcttatcaa agtttgccac gctgtatttg aagacggata tgacacaaag tggaacctca
      721 atggcatgta acaacttcac taatgaaata atccaggggt taacgaacag cgcgcaggaa
      781 aggatacgca acgccataat cacaactccg ataagtaatg cattttttgg ccctacccga
      841 ttcacaaaga aaggaataat cgccatgcac agcgcttcga gtaccacctg gaatgagttg
      901 agataaccat acaggcgcgt tcctacatcg tgtgattcga ataaacctgc ataaaagaca
      961 ggaaaaagtt gttgatcaaa aatgttatag aaagaccacg tccccacaat aaatatgacg
     1021 aaaacccaga agtttcgatc cttgaaaact gcgataaaat cctctttttt tacccctccc
     1081 gcatctgccg ctacgcactg gtgatcctta tctttaaaac gcatgttgat catcataaat
     1141 acagcgccaa atagcgagac caaccagaag ttgatatggg gactgatact aaaaaatatg
     1201 ccggcaaaga acgcgccaat agcatagcca aaagatcccc aggcgcgcgc tgttccatat
     1261 tcgaaatgaa aatttcgcgc cattttttcg gtgaagctat caagcaaacc gcatcccgcc
     1321 agatacccca agccaaaaaa tagcgccccc ggaattagac ctacagaaaa attgctttgc
     1381 agtaacggtt cataaacgta aatcataaac ggtccggtca agaccaggat gaaactcata
     1441 caccagatga gcggtttctt cagaccgagt ttatcctgaa cgatgccgta gaacatcata
     1501 aatagaatgc tggtaaactg gttgaccgaa taaagtgtac ctaattccgt ccctgtcaac
     1561 cctagatgtc ctttcagcca aatagcgtat aacgaccacc acagcgacca ggaaataaaa
     1621 aagagaaatg agtaactgga tgcaaaacga tagtacgcat ttctgaatgg aatattcagt
     1681 gccataatta cctgcctgtc gttaaaaaat tcacgtccta tttagagata agagcgactt
     1741 cgccgtttac ttctcactat tccagttctt gtcgacatgg cagcgctgtc attgcccctt
     1801 tcgccgttac tgcaagcgct ccgcaacgtt gagcgagatc gataattcgt cgcatttctc
     1861 tctcatctgt agataatccc gtagaggaca gacctgtgag taacccggca acgaacgcat
     1921 ctcccgcccc cgtgctatcg acacaattca cagacattcc agcaaaatgg tgaacttgtc
     1981 ctcgataaca gaccaccaca ccttctgcac ctttagtcac caacagcatg gcgatctcat
     2041 actcttttgc cagggcgcat atatcccgat cgttctgtgt ttttccactg ataagtcgcc
     2101 attcttcttc cgagagcttg acgacatccg ccagttgtag cgcctgccgc aaacacaagc
     2161 ggagcaaatg ctcgtcttgc catagatctt cacgaatatt aggatcgaag ctgacaaaac
     2221 ctccggcatg ccggatcgcc gtcatcgcag taaatgcgct ggtacgcgaa ggctcggcag
     2281 acaacgcaat tgaacagaga tgtaaccatt cgccatgtcg ccagcagggc aagtctgtcg
     2341 tctctaaaaa aagatcggca ctggggcgga ccataaacgt aaatgaacgt tccccttgat
     2401 cgttcagatc gacaagcacc gtggatgtcc ggtgccattc atcttgcttc agatacgtga
     2461 tatcgactcc ctcagttagc agcgttcttt gcattaacgc accaaaagga tcatccccca
     2521 cccgacctat aaacccactt gttccgccta atctggcgat tcccaccgca acgttagctg
     2581 gcgcgccgcc aggacaaggc agtaggcgcc cgtctgattc tggcaagaga tctacgaccg
     2641 catcccctaa aacccatact ttggctgaca tttttttccc ttaaattcat ctgagttacg
     2701 catagtgata aacctctttt tcgcaaaatc gtcatggatt tactaaaaca tgcatattcg
     2761 atcacaaaac gtcatagtta acgttaacat ttgtgatatt catcgcattt atgaaagtaa
     2821 gggactttat ttttataaaa gttaacgtta acaattcacc aaatttgctt aaccaggatg
     2881 attaaaatga cgcaatctcg attgcatgcg gcgcaaaacg ccctagcaaa acttcatgag
     2941 caccggggta acactttcta tccccatttt cacctcgcgc ctcctgccgg gtggatgaac
     3001 gatccaaacg gcctgatctg gtttaacgat cgttatcacg cgttttatca acatcatccg
     3061 atgagcgaac actgggggcc aatgcactgg ggacatgcca ccagcgacga tatgatccac
     3121 tggcagcatg agcctattgc gttagcgcca ggagacgata atgacaaaga cgggtgtttt
     3181 tcaggtagtg ctgtcgatga caatggtgtc ctctcactta tctacaccgg acacgtctgg
     3241 ctcgatggtg caggtaatga cgatgcaatt cgcgaagtac aatgtctggc taccagtcgg
     3301 gatggtattc atttcgagaa acagggtgtg atcctcactc caccagaagg aatcatgcac
     3361 ttccgcgatc ctaaagtgtg gcgtgaagcc gacacatggt ggatggtagt cggggcgaaa
     3421 gatccaggca acacggggca gatcctgctt tatcgcggca gttcgttgcg tgaatggacc
     3481 ttcgatcgcg tactggccca cgctgatgcg ggtgaaagct atatgtggga atgtccggac
     3541 tttttcagcc ttggcgatca gcattatctg atgttttccc cgcagggaat gaatgccgag
     3601 ggatacagtt accgaaatcg ctttcaaagt ggcgtaatac ccggaatgtg gtcgccagga
     3661 cgactttttg cacaatccgg gcattttact gaacttgata acgggcatga cttttatgca
     3721 ccacaaagct ttttagcgaa ggatggtcgg cgtattgtta tcggctggat ggatatgtgg
     3781 gaatcgccaa tgccctcaaa acgtgaagga tgggcaggct gcatgacgct ggcgcgcgag
     3841 ctatcagaga gcaatggcaa acttctacaa cgcccggttc acgaagctga gtcgttacgc
     3901 cagcagcatc aatctgtctc tccccgcaca atcagcaata aatatgtttt gcaggaaaac
     3961 gcgcaagcag ttgagattca gttgcagtgg gcgctgaaga acagtgatgc cgaacattac
     4021 ggattacagc tcggcactgg aatgcggctg tatattgata accaatctga gcgacttgtt
     4081 ttgtggcggt attacccaca cgagaattta gacggctacc gtagtattcc cctcccgcag
     4141 cgtgacacgc tcgccctaag gatatttatc gatacatcat ccgtggaagt atttattaac
     4201 gacggggaag cggtgatgag tagtcgaatc tatccgcagc cagaagaacg ggaactgtcg
     4261 ctttatgcct cccacggagt ggctgtgctg caacatggag cactctggct actgggttaa
     4321 cataatatca ggtggaacaa cggatcaaca gcgggcaagg gatccgcgtc actcttcccc
     4381 cttcacgacc ttcaataata tgcaatgcag cttcccgccc gataatgtca tgtggaagct
     4441 gaattgtggt cagcggcggt aaaaacagat gcccgacgcc aaccagatta tcaaagccca
     4501 ttacggcgac atcctgcggg atacgtaccc ccttcgccag gagaacctga taagcgacaa
     4561 aggctgcgcg atcgttacca catatcagaa catcaaaatc tggtttgccc ggtttgaagt
     4621 gggcattgag taaacttgcg agatcggtgt agtgatcatc acctgttgcc atgtgaaatt
     4681 gtttcacctc agccagatct cgtccagcat cacgccaggc ctgctcaaac ccctgccgac
     4741 gataccctgt tgccaacgca ctttccggta gccagaagca taacggttga cgatagcccg
     4801 ccgcgagcaa atgctgtgtt gattcatatt gtgcagtgta atcatcaggg atataactgg
     4861 gtaacgctgg gtcatccgcc acacagttcg ccaatacaat attttcacca tacagagact
     4921 caggcagcgt gatatgtcgc agccccattg tagtatagat aatgccatcc ggacggtggg
     4981 caagcagctg acgtgccgcg cgggcagcgt catcttcaga aaaaatattg attaaaaaac
     5041 tattccagcc gaactcgctg gcggtttgct caatagcaag cagaatatca acagagaaag
     5101 gagtggtagc cgtgtcctgc gccagcacgg cgagagtcga cggcttacgt ccttgagcgc
     5161 gcatcttacg ggcggaaaga tcaggaacat aattcagggt ctggattgcc tgcaatacgc
     5221 ggtcacgcgt tgcaggacgc acagattctg cattatgcat cacccgggag actgtcatca
     5281 tcgacactcc cgccaggcgt gcgacatcct ttaatgaagc catacccaag ccgtttgccg
     5341 taaaacgggc actgtagcag aaacagacgt cactggcgag atccaacgat attctcaacg
     5401 ccctatcacc caacacggca atacaataaa aaataacaat aattgccgga taattgtact
     5461 caattccgcc tctgttctcg ccaacgagta accatatagg caagtaagta aaacatgcca
     5521 actggcatcg ccaacctgga gaagaaaata taaatatgga tcattgtgct gccgctgtat
     5581 cccgacatca gcccggaaat tattgcgatt gctatcggtt caggtgcaat tggctgcacg
     5641 atcgttacgg actcgctttt ctggctagtg aagcaatatt gcggcgctac gctcaatgaa
     5701 acatttaaat actatacgac agcgacattt atcgcttcag tcgtcgctct ggcgggcaca
     5761 ttcctgctgt catttatcat ctaagcgcaa agagacgtac tatggaaaac gctaaaatga
     5821 actcgctcat cgcccagtat ccgttggtaa aggatctggt tgctcttaaa gaaaccacct
     5881 ggtttaatcc tggcacgacc tcattggctg aaggtttacc ttatgttggc ctgaccgaac
     5941 aggatgttca ggacgcccat gcgcgcttat cccgttttgc accctatctg gcaaaagcat
     6001 ttcctgaaac tgctgccact ggggggatta ttgaatcaga actggttgcc attccagcta
     6061 tgcaaaaacg gctggaaaaa gaatatcagc aaccgatcag cgggcaactg ttactgaaaa
     6121 aagatagcca tttgcccatt tccggctcca taaaagcacg cggcgggatt tatgaagtcc
     6181 tggcacacgc agaaaaactg gctctggaag cggggttgct gacgcttgat gatgactaca
     6241 gcaaactgct ttctccggag tttaaacagt tctttagcca atacagcatt gctgtgggct
     6301 caaccggaaa tctggggtta tcaatcggca ttatgagcgc ccgcattggc tttaaggtga
     6361 cagttcatat gtctgctgat gcccgggcat ggaaaaaagc gaaactgcgc agccatggcg
     6421 ttacggtcgt ggaatatgag caagattatg gtgttgccgt cgaggaagga cgtaaagcag
     6481 cgcagtctga cccgaactgt ttctttattg atgacgaaaa ttcccgcacg ttgttccttg
     6541 ggtattccgt cgctggccag cgtcttaaag cgcaatttgc ccagcaaggc cgtatcgtcg
     6601 atgctgataa ccctctgttt gtctatctgc cgtgtggtgt tggcggtggt cctggtggcg
     6661 tcgcattcgg gcttaaactg gcgtttggcg atcatgttca ctgctttttt gccgaaccaa
     6721 cgcactcccc ttgtatgttg ttaggcgtcc atacaggatt acacgatcag atttctgttc
     6781 aggatattgg tatcgacaac cttaccgcag cggatggcct tgcagttggt cgcgcatcag
     6841 gctttgtcgg gcgggcaatg gagcgtctgc tggatggctt ctataccctt agcgatcaaa
     6901 ccatgtatga catgcttggc tggctggcgc aggaagaagg tattcgtctt gaaccttcgg
     6961 cactggcggg tatggccgga cctcagcgcg tgtgtgcatc agtaagttac caacagatgc
     7021 acggtttcag cgcagaacaa ctgcgtaata ccactcatct ggtgtgggcg acgggaggtg
     7081 gaatggtgcc ggaagaagag atgaatcaat atctggcaaa aggccgttaa taacgtttca
     7141 acgcagcatc gcaatccttt ccctgggtga gcgatgctgc cgatggcgca gacttaagat
     7201 ccccggtctt acccgctata accccctcct tttatcatca cccaacgcct ttcgctgtaa
     7261 acggcggttt cgcaaaccaa accaaaaccg taagtaagat aaaagcaata gccgccatac
     7321 ggaaaatctc atttgcagaa atagaaagtg actgctgggt tatttcatta ttaatttcat
     7381 taagaactcc tgaaagcgaa ccatagtatt tatccataat ttgtgacgat gaattaaaca
     7441 cggggttaaa ttgatcgatg gttgctgtca actgactatg gtgtaacgat tcgcgtcgtc
     7501 cccacagcgt cattgtcaac gacgtaccaa ctgatcctga caaggtacga aaaaaattac
     7561 tcatactcga ggcattggca aatttattat ctggcaagcc tgaaaacgaa atcgttgtta
     7621 agggtaaaaa gaaacaggca acggcgaatc cctggaaaaa ctgtggcaaa atgatgcctg
     7681 taaaatcaat cgttggcata aatgtcacag aacgccagta atagcaaacc gcatacatca
     7741 aaaaactgaa tgtcactaac acccgcatgt ctattttatt gccataacgt cctatcaaag
     7801 gtgaaatcaa tagtggcatg atgccgatgg gcgcataagc aagcccggcc catatcgcat
     7861 tataccccat cgtttcctgg agtaactgcg gcataaggac gatcgctcca gagtaaaata
     7921 aatacgcgca ggtgatactc acaataccaa tggtgaaatt acgagactta aacaaactga
     7981 gatcaagaat cggattctct gaggtcgact cccaaatgac taaagagatc agagaaataa
     8041 ctgatactac tgttaatatt attattgtac tcgagttgaa ccaatccaga tcgcgccctt
     8101 tatcaagcat aatttgcaag ccaccaacac cgagcactaa cagggtcagt cctggtagat
     8161 tcatttttac cggtgaagtt tcagtttctc ttcctttaag taaggttaag cataatgtca
     8221 ggacgataat ccccataggg acattgatta aaaatatcca accccagcta aagttatcgc
     8281 aaatataacc gcccaatatc ggcccacata tcggagcgat aatcacggtc attgaccata
     8341 atgccagagc aaatgttctt ttttctggtg gataattcct caataataaa ctctgtgaca
     8401 gtggaattaa cggccccgcc attaatccct gaacgactct aaaaaatatc agcacatcaa
     8461 gattggtcga taaactacac attaaggaag acagcgaaaa aaaagtgact gaaagtaaaa
     8521 ataaccttaa ttcgcctatt ctttgtgcca gcctgccggt aacagggatc gcaatggcat
     8581 ttgctacgcc aaacgaggtg ataacccagg tgccttcgtc tgttgatgct cccagaaagc
     8641 cagatattgt cggtattgcg acgttagaaa tagtggaatc caacatttgc ataaatgtcg
     8701 ctaatgacaa tgcaatagtg acgcaccata acgtcccacc ggttaatggt gccggagttg
     8761 atttagtgat tgccattaaa gttgtccatt atgcgaaata atgttgctaa tttctttttc
     8821 tatcggactg gtatcgataa ctaaagcctt actggtataa gccggcatgg aggtcacggt
     8881 tgaagccagc tcaggcatct cggcaatgtc ttcgttcttc gtatcaatag ttgctgtcat
     8941 cgataaacca atacgcaagg gatgttccat gagttctttt ggatcaagag aaacttcaac
     9001 cggtacacgc tgaacgattt tgatccagtt ccctgtcgca ttttgtgcag gtaataagga
     9061 gaacgcattg ccggttccca tattgatccc tgtcacccga ccatgaaaca caacattttc
     9121 accataaaga tcgctgataa tattgaccga ttgaccaatc cgtacatccg taagttgtgt
     9181 ttctttaaag ttggcattaa cccacatttg acgtgccggt acgacagcca ttaacgattg
     9241 tccggggctc actgtttcgc cgacctgaac acttctctgg gcaatatagc cggtaaccgg
     9301 actcttaata tccgtacgtt taagcgccaa ccaggcttct ttagttgcat ccgccgcttc
     9361 aacgacttgt ggctgacggt ttaatggtgt gttcattatt aaagctttat tcgctttata
     9421 agcctggata gcggcattca atgccgcttt gctacttatt aacgtatctt tggtatgctc
     9481 cagcgtttct tttgaaataa ccccctgctt cgctaacggc actcgacggt tataatcttc
     9541 taaagattgt tgatactgaa tacgtgctga agcgacttcg gcactgtatt gtttatcctg
     9601 taagtatagt ttattcgttt gccgaacaat atttgccaga ttatttttag ctttattgag
     9661 tgcgatagtg gcatcagttt tatccagtga aactaaaatg tcaccttgtc gaacgtagtt
     9721 cgtatcttta tgattaacga cagtgacact acctgagact tgtgcagaaa ttggatctgc
     9781 attccccgtg acataggcgt catctgtact aatcatgtct tctaattcca ttgaccaata
     9841 ggcataggca cctgaaaacg caat
//
LOCUS       DAPHDW010000158         9835 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.161, whole genome shotgun sequence.
ACCESSION   DAPHDW010000158 DAPHDW010000000
VERSION     DAPHDW010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9835
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.161"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            155..370
                     /gene="bdm"
                     /locus_tag="RH057_003854"
     CDS             155..370
                     /gene="bdm"
                     /locus_tag="RH057_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-dependent modulation protein"
                     /protein_id="HDV3130156.1"
                     /translation="MFTYYQAENSTAEPALVNAIEQGLRAEHGVVTEDDILMELTKWV
                     EASDNDILSDIYQQTINYVVSGQHPTL"
     gene            472..609
                     /gene="sra"
                     /locus_tag="RH057_003855"
     CDS             472..609
                     /gene="sra"
                     /locus_tag="RH057_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary-phase-induced ribosome-associated
                     protein"
                     /protein_id="HDV3130157.1"
                     /translation="MKSNRQARHILGLDHKISNQRKIVTEGDKSSVVNNPTGRKRPAE
                     K"
     gene            766..2463
                     /gene="maeA"
                     /locus_tag="RH057_003856"
     CDS             766..2463
                     /gene="maeA"
                     /locus_tag="RH057_003856"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HDV3130158.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIISQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            2597..3607
                     /gene="adhP"
                     /locus_tag="RH057_003857"
     CDS             2597..3607
                     /gene="adhP"
                     /locus_tag="RH057_003857"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HDV3130159.1"
                     /translation="MKAAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMAEECIVVADYAVKVPDGLDSAAASSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMGADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RR"
     gene            3754..4038
                     /locus_tag="RH057_003858"
     CDS             3754..4038
                     /locus_tag="RH057_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HDV3130160.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVDVSRLRRLVTQ"
     gene            complement(4089..4742)
                     /gene="fdnI"
                     /locus_tag="RH057_003859"
     CDS             complement(4089..4742)
                     /gene="fdnI"
                     /locus_tag="RH057_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HDV3130161.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4735..5619)
                     /gene="fdnH"
                     /locus_tag="RH057_003860"
     CDS             complement(4735..5619)
                     /gene="fdnH"
                     /locus_tag="RH057_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HDV3130162.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5632..8679)
                     /gene="fdnG"
                     /locus_tag="RH057_003861"
     CDS             complement(5632..8679)
                     /gene="fdnG"
                     /locus_tag="RH057_003861"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(8092..8094),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HDV3130163.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPEYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGVLRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETLAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8911..9792
                     /gene="yddG"
                     /locus_tag="RH057_003862"
     CDS             8911..9792
                     /gene="yddG"
                     /locus_tag="RH057_003862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415990.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HDV3130164.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEELGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEICLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGDNGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGASLWVYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYAAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
BASE COUNT         2378 a         2572 c         2494 g         2391 t
ORIGIN      
        1 ataataccta tcaatatcat gaggttaaaa tataaaaatc atcaaccagg actaatctta
       61 acaacgaagc ggcaaatatt tgccatgctg aatgtgcttt ataaatctgc gatccgtagc
      121 agacaccata aatacacaga cacggagaat cactatgttt acttattatc aggcagaaaa
      181 ttcaacagca gaacccgctc tggttaatgc cattgagcaa ggccttcggg cagagcacgg
      241 tgttgtcact gaagatgaca ttctcatgga gctgaccaaa tgggtggaag cttctgataa
      301 cgacatcctc agtgatatct accagcaaac gatcaattat gtggtcagtg gccagcaccc
      361 tacgctttaa ggtgctatgc ttgatcggca acctaattta ggggtttagc acgtgtttct
      421 tcgctacggc gatgttgtcc ttaaaactag caacaggatt gaggagttaa aatgaaatcg
      481 aaccgtcagg cacgtcatat tcttggactg gaccataaaa tttctaacca gcgcaaaata
      541 gttaccgaag gtgacaaatc cagcgtggta aataatccaa ccggcagaaa acgccccgct
      601 gaaaagtaat tcataaccat cagtcctcaa tgacgattaa acaccattgc ctgcgcaatg
      661 gtgtttttgt ttttatctgc tctatacttg gggccgacgc cctggcggta aagcaaagac
      721 gataaaagcg tgccagggat ggatattcaa aaaagagtga gtgacatgga accaaaaaca
      781 aaaaaacagc gttcgcttta tatcccttac gctggccctg tactgctgga atttccgttg
      841 ttgaataaag gcagtgcctt cagcatggaa gaacgccgta acttcaacct gctggggtta
      901 ctgccggaag tggtcgaaac catcgaagaa caagcggaac gagcatggat ccagtatcag
      961 ggattcaaaa ccgaaatcga caaacacatc tacctgcgta acatccagga cactaacgaa
     1021 accctcttct accgtctggt aaacaatcat cttgatgaga tgatgcctgt tatttatacc
     1081 ccaaccgtcg gcgcagcctg tgagcgtttt tctgagatct accgccgttc acgcggcgtg
     1141 tttatctctt accagaaccg gcacaatatg gacgatattc tgcaaaacgt gccgaaccat
     1201 aatattaaag tgattgtggt gactgacggt gaacgtattc tagggcttgg tgaccagggc
     1261 atcggcggga tgggcattcc gatcggtaaa ctgtcgctct ataccgcctg tggcggcatc
     1321 agcccggcgt atacccttcc ggtggtgctg gatgtcggaa cgaacaacca acagctgctt
     1381 aacgatccgc tgtatatggg ctggcgtaat ccgcgtatca ctgacgacga atactatgaa
     1441 ttcgttgatg aatttatcca ggctgtgaaa caacgctggc cagacgtgct gttgcagttt
     1501 gaagactttg ctcaaaaaaa tgcgatgccg ttacttaacc gctatcgcaa tgaaatttgt
     1561 tcttttaacg atgacattca gggcactgcg gcggtaacag tcggcacact gatcgcagca
     1621 agccgcgcgg caggtggtca gttaagcgag aaaaaaatcg tcttccttgg cgcaggttca
     1681 gcgggatgcg gcattgccga aatgatcatc tcccagaccc agcgcgaagg attaagcgag
     1741 gaagcggcgc ggcagaaagt ctttatggtc gatcgctttg gcttgctgac cgacaagatg
     1801 ccgaacctgc tgcctttcca gaccaaactg gtgcagaagc gcgaaaacct cagtgactgg
     1861 gataccgaca gcgatgtgtt gtcactgctg gatgtggtgc gcaatgtaaa accagatatt
     1921 ctgattggcg tctcaggaca gaccgggctg tttacggaag agatcattcg tgagatgcat
     1981 aaacactgtc cgcgtccgat cgtgatgccg ctgtctaacc cgacgtcacg cgtggaagcc
     2041 acaccgcagg acattatcgc ctggaccgaa ggtaacgcgc tggtcgccac tggcagcccg
     2101 tttaatccag tggtatggaa agataaaatc taccctatcg cccagtgtaa caacgccttt
     2161 attttcccgg gcatcggcct gggggttatt gcttccggcg cgtcacgtat caccgatgag
     2221 atgctgatgt cggcaagtga aacgcttgct cagtattcgc cgctggtcct gaacggcgaa
     2281 ggtctggtac taccggaact gaaagatatt cagaaagtct cccgcgcaat tgcgtttgcg
     2341 gttggcaaaa tggcgcagca gcaaggcgtg gcggtgaaaa cgtctgccga agctctgcaa
     2401 caggccattg atgataattt ctggcaagcc gaataccgcg actaccgccg tacctccatc
     2461 taagcctgcg cccggtagtg aaggctaccg ggctatttct ctctcccttt ttcagatctc
     2521 atccatactg ggtagtggcg aataaatctc atttgcctca cctgctatgc agaacatcat
     2581 ccgaaaagga ggaactatga aggctgcagt tgttacgaag gatcatcacg ttgacgttac
     2641 ggataaaaca ctgcgctcac tgaaacatgg cgaagccctg ctgaaaatgg agtgttgtgg
     2701 tgtatgtcat accgatcttc atgttaagaa tggcgatttt ggtgacaaaa ctggcgtaat
     2761 tctgggccat gaaggcatcg gtgtggtggc agaagttggc ccaggtgtca cctcattaaa
     2821 accaggcgat cgtgccagcg tggcgtggtt ctacgaagga tgcggtcatt gcgaatactg
     2881 taacagtggt aacgaaacgc tctgccgttc agttaaaaat gccggataca gcgttgatgg
     2941 cgggatggcg gaagagtgca tcgtggtcgc cgattacgcg gtaaaagtgc cagatggtct
     3001 ggactcggcg gcggccagca gcattacctg tgcgggggtc accacctaca aagccgttaa
     3061 actgtcaaaa attcgtccag ggcagtggat tgctatctac ggtcttggcg gtctgggtaa
     3121 cctcgctctg caatacgcga agaatgtctt taacgcgaaa gtgatcgcca ttgatgtcaa
     3181 tgatgagcag ttaaaactgg caaccgaaat gggtgcagat ttagcgatta actcacgcac
     3241 cgaagacgcc gccaaaattg tgcaggagaa aaccggtggc gctcacgctg cggtggtaac
     3301 agcagttgct aaagctgcgt ttaactcggc agttgatgct gtccgtgcag gcggtcgtgt
     3361 tgtggctgtc ggtctgccgc cggagtctat gagcctggat atcccacgcc ttgtgctgga
     3421 tggcattgaa gtggtcggtt cgctggtcgg cacgcgccag gatctaaccg aagccttcca
     3481 gtttgccgcc gaaggcaaag tggtgccgaa agtcgccctg cgtccgttag cggacatcaa
     3541 caccatcttt accgagatgg aagaaggcaa aatccgtggc cgtatggtga ttgatttccg
     3601 ccgttaagag gcctttgctg cgagtgccag tcctggtgtc gcagcatcgc actctctccg
     3661 acattaaatg gctaaatgga ttattgttcc ttcctacgca acaataatga tagtgttcct
     3721 gatgaaggaa cactactgag agtaaagaca ctgatgaaaa tggccaatca tccccgcccg
     3781 ggggacatta ttcaggaatc gctggacgaa cttaatgtca gcctgcgcga gtttgccaga
     3841 gcaatggaaa ttgctccctc aacggcaagc cgattgctga ccggaaaagc agctttgaca
     3901 ccagaaatgg cgattaaact ttccgtggtg atcggcagtt cgccgcaaat gtggctgaat
     3961 ctgcaaaacg cctggagtct ggcagaggca gaaaaaacgg tggatgtgtc gcgactacgc
     4021 cgtctggtta cgcaataaaa aaatggcgct tcttaaagcg ccattttcag cttaaagttc
     4081 aaaagagatt atatcccttc ttcactctct tttttcgctt ctgccttctc gatttcacga
     4141 taccagcgcg gatggtgttt cttcgcccag cgacggctca ccttcccttc gatcatccct
     4201 ttaatcgacc ctttcaccca gaatgccata tacatatgga tcaggatggc gtggatcagg
     4261 atgatacccg cagccgcgtg gatcagcagg ctgtagcgaa caacctgcat cgggaagtac
     4321 tgcgcaaagt acggacgcca gataatcacc ccggtcacca gcagcacgaa aatcatgctc
     4381 atgatcgacc agaacatcat cttttgcccg gcgttgtact taccgacatc cgccacttta
     4441 tgctcattgc ctttcaatac ttcgacaatg ttcaacagcc acggaatatc tttcttatcc
     4501 gggatgttgt gatgcacaaa acgcacaaac ataaacatca gtgcgacgaa aatcgcaatg
     4561 ccgaaaaacg ggtgcaaaat gcgtcccatc tgcggcgtac cgaaggtttg cgtcagccat
     4621 tgcagcgtcg ggaagaagaa cgaaatcccg gacagcgcca ccaggaagaa gcaaatcacc
     4681 acggtccagt gacaggcgcg atcaataaat ttggtgcgca caatcatttt cgacttactc
     4741 atgatgatcc tcctcgtcat cgtccacttc cttattcggg ccaataccga tgtagtggaa
     4801 aatcaacccg gcaaaagtgg caataaagcc agccgctgcc agcggtttca acgcgccttt
     4861 ccacaggctt accgaggtgt cgatcttcgg atctttcggc agaccgtgat acagctccgg
     4921 ctgatcggcg tgatgcagca cgtacataac gtgcgtacca ccgacccctt ccgggttgta
     4981 gacgccagca tgttcgtaac cacgcgcttt cagtttcgcc acgcgctgtt ccgccagctc
     5041 cagcatctcc ttcttggtgc cgaagtggat agccccggtc ggacaggttt tcacacaagc
     5101 cggttcctgg ccgacgctga cgcgatcgac gcagagcgtg catttatata cccggttatc
     5161 ctctttgttg aggcgcggaa tattaaacgg acacccggca atgcagtaac cacagccgat
     5221 gcagttttcc gactggaaat cgacaatccc gttagcgtac tgaatgattg caccagcaga
     5281 cgggcacgcc ttcaggcagc cgggatcttc acagtgcata cagccgtctt tacggatcag
     5341 ccactccagc ttgccgttct gttcggtttc gctaaagcgc atcaccgtcc aggacttggc
     5401 gctcagatcg gcggggttat cgtaaacccc gacgcagtgc cccacttcat cacggatgtc
     5461 gttccactcc gaacacgcca cctgacaggc tttacagccg atacaggtgg aaacgtcgat
     5521 aagttttgcg acttctgctt tgtaatcacg cacctgagaa ggcggcgtga tggagttagt
     5581 tgcggacctt ttgataatgt cctgcgtttc catagccatc tgttcgcccc cttacgcctt
     5641 ctcgatgttg actaagaacg ctttatattc cggcgtttgc gagtttgcat caccgacatt
     5701 cggcgtcaga gtgttagcga tataaccttt acgcgcgaca ccctcaaagc cccagtggat
     5761 tggaataccc accgtttcaa cctgctgacc atttacattc agtggtttca gacgacgcgt
     5821 taccacagcc accgcgcgga taaagccacg cttgcttgag acagtgacac gatcgccatt
     5881 attaatgcct ttcgccgccg ccagcgtttc gctgatttcc acaaactgtt ccggctgagc
     5941 aattgcgttg agcaatgcgt gcttggtcca ggtgtggaag tgctcggtca gacgataggt
     6001 cgtacccaca tacgggaact gctctttttt acccatccgc agtgcgtctt gttcatacag
     6061 acgaacaacc gggttagaca ccacgttcgg atgcagcggg ttagtgccca gcggcgtttc
     6121 aatcggctcg tagtgttccg ggaacggacc ttccgccatt ttgttgatgg caaacaggcg
     6181 tcccatccct tccggctgca tgataaacgg cccggttggc gtacccggtg cggcattgcc
     6241 gaagtcagga atatcgttac ccgtccactt gctgccgttc cactggatca gcatccgttt
     6301 cggatcccac ggtttaccgt tgatatccgc cgaagcacgg ttgtacagca cgcgacggtt
     6361 gagcggccac gcccaggccc atcccagcgt attccccaga ccggacgggt cggagttatc
     6421 gcggttagcc atctggttgc cctgctctgt ccagctaccg gtgtagatcc agcaagaaga
     6481 tgcggtggta ccgtcatcac gcagatgcgc aaagctactc agcaactgac ctttcttcgc
     6541 aatcagcacg ccattagcat catagagatc ttccagcgca tagccgttgt tctctttagc
     6601 tacttcgtca gattgcggtt cgtgcggctg cttgtagttc cagctcatct tcatcagcgg
     6661 ttctacgcct ttaccacctt cggcctggta cagctcgcgc aggtgatggt agatacccgc
     6721 cagaatttca ccgtcgttac gcgcttcgcc cggcgcatcc tgacctttcc agtgccactg
     6781 cagccagcga ccggagttag caatagaacc atcttcttca gcaaagcagg tcgaaggcag
     6841 acggaatact tcagtctgaa tagacgccgg atcgacatcg ttcgactcgc cgtggttctg
     6901 ccagaaggtt gaggtttcag tcaccagcgg atcgataacc accatgtact tcagcttgct
     6961 taggcagctc accactttgt ttttgtccgg gaaggacgca accgggttaa agccctggca
     7021 gaaataaccg gtgactttgc cttcatccat catgttgaaa tacttgatga cgtcgtaggt
     7081 ctggtcccac ttcggcagcc agtcatagcc ccagttgttc tctttctgcg cggcatcgcc
     7141 atagaaagat ttcatcaggc taacgaagaa cttcggatag ttgctccagt agttcacctg
     7201 atcagccagc gtcgctttcg gcgtgttcgc ttccagatac gactgcaaat caacctgttt
     7261 ttctgacggc agcgtcagat aacctggcag gctggtagag agcagaccta agtcagtcag
     7321 gccctgaatg ttggagtgac cacgcaatgc gttcacgccg ccaccggcca tacccatgtt
     7381 accgagcagt aactggatca tcgccatagt acggatgttc tgcgcaccca cggtgtgctg
     7441 cgtccagccc agcgcgtaca ggaaggtggt tgtgcgatcc ggtgcgctgg tggaggccag
     7501 cacttcacac actttcagga agtcggcttt tggcgtaccg cagatgtttt caacgacgtc
     7561 cggcgtgtag cgggaaacgt gcgctttcag caggttccac acacagcgcg gatgagtcag
     7621 tgtttcatcg cgtttcgcat agccgttttc atcgaactga tagttccagg acgatttgtc
     7681 gtactggcgt ttttcagcgt cataaccgct gaacaggcca tcttcgaaag caaaatcatc
     7741 acgcaccagc aggctggcgt tggtgtaatg tttaacgtat tcggcgttga ttttgttgtt
     7801 ttcgatcagg tagcgcaaaa cgccagacag gaacgtaatg tccgtaccgg aacgaatagg
     7861 tgcgtaaata tccgccacag aagcggtacg cgtaaaacga ggatcgacaa cgatcaaggt
     7921 tgcatcgttg ttgtttttcg cttccatcgc ccagcggaaa ccgacgggat gcgcttcagc
     7981 agcgttaccg cccattacca ttacgacgtt agcgtttttg atatccaccc agtggttggt
     8041 catcgcaccg cgaccaaatg ttggagcaag acttgctacc gttggtccgt gtcagacgcg
     8101 cgcctggttg tctaccgcca gcatcccgag ggagcgggca aatttctgtg tcagcatccc
     8161 ggtttcgttg ctggcaccgg aggcacacag cataccggta gaaagccaac ggtttaccgt
     8221 tacgccctgc tcgttctttt caataaagtt agcgtcacgg tcagctttca tcagctttgc
     8281 aatacgggag aatgcttctt cccagctaat gcgctgccat ttgtcagaac ctggcgcacg
     8341 atattccggg tagcgcagac ggttttcgct gttgacgtaa tccagcaaac cggccccttt
     8401 tgggcacagc gcaccacggc ttaccggatg atccgggtca ccttcaatgt gataaatcgc
     8461 ttctctggcg tttttcgcgc catcacccag gctatacatc aatagcccgc aacctacgga
     8521 acagtatgtg caggtgttac ggatctcttt agcgcgtaat aatttgtagt ttcgcgcctg
     8581 agccagtgct tgcttcgggg caaagcccaa tgccgctacc gttgttccag ccataccgcc
     8641 cgcgcagatt ttaaaaaatt gtctgcgact gacgtccatt gctttcctct tttttcaggg
     8701 gtattactgc gcgaggaaac taacagtaaa attctgaatc tttttgcgcg aaatcaaaaa
     8761 gaacacgtgt ttgccacaaa ataatccgcc tgcgcaaatt gacctacctc aatagcggta
     8821 gaaaaacgca ccactgcctg acaggccagt taaaaaaatg ctataaaatt cagcttaatt
     8881 tttaacggca agagagacaa aacagcgagc atgacacgac aaaaagcaac gctcataggg
     8941 ctaatagcga tcgtcctgtg gagcacgatg gtaggattga ttcgcggtgt cagcgaggag
     9001 ctcggcccgg tcggcggcgc agctgctatc tattcattaa gcgggctgct gttaatcttc
     9061 acggttggat ttccgcgtat tcggcagatt ccgaaaggct atttactcgc cgggagtctg
     9121 ttattcgtca gctatgaaat ctgtctggcg ctttccttag ggtatgcggc gacccgtcat
     9181 caggcgattg aagtgggtat ggtgaactat ctatggccca gcctgacaat tctctttgcc
     9241 attctgttta atggtcagaa aaccaactgg ctgattgtac caggattatt attagccctg
     9301 gtcggcgtct gctgggtgtt aggcggtgat aatgggttac attatgatga aatcatcaat
     9361 aatattacca ccagcccatt gagttatttc ctggcgttta ttggtgcgtt tatctgggca
     9421 gcctattgca cagtaacgaa taaatacgca cgcggattta acggaattac cgtttttgtc
     9481 ctgctaacgg gagcaagtct gtgggtttac tattttctca cgccacaacc agaaatggta
     9541 tttagcacgc ccgtcatgat taaactcatc tctgcggcat ttaccttagg atttgcttat
     9601 gctgcatgga atgtcggtat actgcatggc aatgtcacca taatggcggt aggctcgtat
     9661 tttacgcccg tactttcctc agcgcttgca gccgtgctgc tcagcgcccc gctgtcgttc
     9721 tcattctggc aaggcgcgct aatggtctgc ggcggttccc tgctctgctg gctggcgaca
     9781 cgtcgtggtt aaaataattg ccgggtctac ggcccggcag ttaatcagat aaatt
//
LOCUS       DAPHDW010000159         9631 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.162, whole genome shotgun sequence.
ACCESSION   DAPHDW010000159 DAPHDW010000000
VERSION     DAPHDW010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.162"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(115..1758)
                     /gene="yeeO"
                     /locus_tag="RH057_003863"
     CDS             complement(115..1758)
                     /gene="yeeO"
                     /locus_tag="RH057_003863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416491.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
                     /protein_id="HDV3130165.1"
                     /translation="MLRHILTAKNLLSNPIFKFPNCLPFLSTVCCICRQFVGENLCSF
                     ADSPSLFEMWFHFLQLRSALNISSALRQVVHGTRWHAKRKSYKVLFWREITPLAVPIF
                     MENACVLLMGVLSTFLVSWLGKDAMAGVGLADSFNMVIMAFFAAIDLGTTVVVAFSLG
                     KRDRRRARVATRQSLVIMTLFAVLLATLIHHFGEQIIDFVAGDATTEVKALALTYLEL
                     TVLSYPAAAITLIGSGALRGAGNTKIPLLINGSLNILNIIISGILIYGLFSWPGLGFV
                     GAGLGLTISRYIGAVAILWVLAIGFNPALRISLKSYFKPLNFSIIWEVMGIGIPASVE
                     SVLFTSGRLLTQMFVAGMGTSVIAGNFIAFSIAALINLPGSALGSASTIITGRRLGVG
                     QIAQAEIQLRHVFWLSTLGLTAIAWLTAPFAGVMASFYTQDPQVKHVVVILIWLNALF
                     MPIWSASWVLPAGFKGARDARYAMWVSMLSMWGCRVVVGYVLGIMLGWGVVGVWMGMF
                     ADWAVRAVLFYWRMVTGRWLWKYPRPEPQKCEKKPVVSE"
     gene            1763..1838
                     /locus_tag="RH057_003864"
     tRNA            1763..1838
                     /locus_tag="RH057_003864"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1796..1798,aa:Asn,seq:gtt)
     gene            complement(1876..2826)
                     /gene="cbl"
                     /locus_tag="RH057_003865"
     CDS             complement(1876..2826)
                     /gene="cbl"
                     /locus_tag="RH057_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HDV3130166.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEL
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGTPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFRWHHSLLVPHDHPLTQISPLTLESIAKWPLITYRQGITGRSRIDDAFARKGLLA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMESSEEEIDYQI"
     gene            complement(2928..3845)
                     /gene="nac"
                     /locus_tag="RH057_003866"
     CDS             complement(2928..3845)
                     /gene="nac"
                     /locus_tag="RH057_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HDV3130167.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVATLEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQALSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEIVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQSVDVNAIAQMNLFLPSDYSAIRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            4172..4247
                     /locus_tag="RH057_003867"
     tRNA            4172..4247
                     /locus_tag="RH057_003867"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4205..4207,aa:Asn,seq:gtt)
     gene            complement(4303..5238)
                     /gene="ldtA"
                     /locus_tag="RH057_003868"
     CDS             complement(4303..5238)
                     /gene="ldtA"
                     /locus_tag="RH057_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV3130168.1"
                     /translation="MMRRVNILCSFALLFASHTSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKSGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNTRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSNWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQRRSGMPVQISSGSRQMF"
     gene            complement(5300..6379)
                     /gene="cobT"
                     /locus_tag="RH057_003869"
     CDS             complement(5300..6379)
                     /gene="cobT"
                     /locus_tag="RH057_003869"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HDV3130169.1"
                     /translation="MQTLADLLNTTPAIDPAAMSRAQRHIDGLLKPVGSLGRLEALAI
                     QLAGMPGLNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVVDVGIDSAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGMAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMEMRLGEGSGAALAMPIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(6391..7134)
                     /gene="cobS"
                     /locus_tag="RH057_003870"
     CDS             complement(6391..7134)
                     /gene="cobS"
                     /locus_tag="RH057_003870"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HDV3130170.1"
                     /translation="MSKLFWAMLSFITRLPVPRRWSQGLDFEHYSRGIITFPLIGLLL
                     GAISGLVFMVLQAWCGVPLAALFSVLVLALMTGGFHLDGLADTCDGVFSARSRDRMLE
                     IMRDSRLGTHGGLALIFVVLAKILVLSELALRGEPILASLAAACAVSRGTAALLMYRH
                     RYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVLLPGMHGVAAMVVTMVAIFILGQL
                     LKRTLGGQTGDTLGAAIELGELVFLLALL"
     gene            complement(7131..7676)
                     /gene="cobU"
                     /locus_tag="RH057_003871"
     CDS             complement(7131..7676)
                     /gene="cobU"
                     /locus_tag="RH057_003871"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HDV3130171.1"
                     /translation="MMILVTGGARSGKSRHAEALIGDSSQVLYIATSQILDDEMAARI
                     EHHRQGRPAHWRTVERWQHLDELIHADINPHEAVLLECVTTMVTNLLFDYGGDKDPDE
                     WDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNEVGMGIVPESRLARHFRDIAGRVNQ
                     QLAAAANEVWLVVSGIGVKIK"
     gene            9382..>9631
                     /locus_tag="RH057_003872"
     CDS             9382..>9631
                     /locus_tag="RH057_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130172.1"
                     /translation="MSGKRYPEEFKTEAVKQVVDRGYSVASVATRLDITTHSLYAWIK
                     KYGPDSSTNKEQSDAQAEIRRLQKELKRVTDERDILKKA"
BASE COUNT         2698 a         2542 c         2049 g         2342 t
ORIGIN      
        1 tgaactacag aggaatcgtg agaacgaggc gaatattagc gatgcccacc cacaatgtca
       61 aagcctgttt tttaaatttg aaatcgtttg ctgaaataat ctgcattttg tcgtttattc
      121 cgacacaact ggcttttttt cacacttttg cggctcgggt cgagggtatt tccatagcca
      181 acgtccagta accattcgcc agtaaaacag cacggcccgc acagcccagt cggcaaacat
      241 tcccatccag acaccaacca caccccagcc aagcatgatt cccagcacat aaccgactac
      301 aacccgacaa ccccacatgc tcaacatcga aacccacatg gcgtaacggg catcacgagc
      361 acctttaaat ccagcgggta gcacccatga ggcggaccaa ataggcataa ataaagcatt
      421 tagccaaatc agaatcacaa cgacatgttt aacctgtgga tcctgggtgt aaaacgatgc
      481 cataaccccg gcaaagggag ccgttagcca ggcgatggcc gttaatccaa gagtggaaag
      541 ccagaacaca tgccgcaact gaatctctgc ttgcgctatc tgccctaccc ccaaccttcg
      601 gcctgtaatg atcgtagaag cagagccgag cgcacttccg ggtaagttga taagagccgc
      661 aattgaaaac gcgataaaat ttccggcaat aacactggtc cccatcccgg caacgaacat
      721 ttgggttaat aaccgaccac tggtaaataa cactgattcg acactcgcgg gaataccaat
      781 ccccatgact tcccagataa tgctaaaatt cagcggttta aaatagctct ttaacgaaat
      841 ccttagcgca ggattaaaac caatcgccag cacccacaaa attgcaactg cgccaatata
      901 acgagaaatg gttaaaccca gccctgcccc gacaaatccc agtcccggcc aggagaaaag
      961 gccgtaaatc aatatgccgc taataataat attaagaata ttcaggctac cgttaatcaa
     1021 tagcggtatt ttcgtattcc ctgcaccacg aagtgccccg ctaccaataa gagtgatggc
     1081 agctgctgga taactgagta ccgtcagctc cagataagtc aacgccagtg ctttaacttc
     1141 tgtcgtggca tcacccgcga cgaaatcaat aatttgttcg ccaaaatgat gaataagcgt
     1201 tgccaacagt acggcaaaca acgtcatgat caccaatgac tgccgcgtcg ccaccctcgc
     1261 tcgtcgtcga tcccgcttac cgagactaaa tgccacaacg acagtagtac caagatcgat
     1321 agcagcaaaa aaagccataa tgaccatatt gaagctgtcc gccaatccca cgccggccat
     1381 cgcatctttt cccagccagc tgaccagaaa agtgctcaga acccccatca acaggacaca
     1441 ggcattctcc atgaagatag gaacagcaag cggggttatc tcgcgccaga acaacacttt
     1501 gtagctcttg cgtttagcgt gccagcgagt gccgtgaaca acctggcgta aagcagagga
     1561 gatattcaaa gccgacctta attgcagaaa gtgaaaccac atttcaaata atgagggaga
     1621 atcagcaaag ctgcaaagat tttcgccaac aaattgtctg caaatgcaac aaactgttga
     1681 tagaaacggc aaacagttgg ggaatttaaa aatcgggttt gacaaaagat ttttcgccgt
     1741 taagatgtgc ctcaacaacg attcctctgt agttcagtcg gtagaacggc ggactgttaa
     1801 tccgtatgtc actggttcga gtccagtcag aggagccaaa ttcctgaaaa gcccgctttt
     1861 atagcgggat ttttgctata tctgataatc aatttcctct tcactgcttt ccatcacctg
     1921 ccgcttgata tcctcaactg acagtcctgc attacaaagt tccagaaagc gccagacata
     1981 gttacgctga agttgtcctc gcttcagtcc caaccagaca gtattagcat caaaaagatg
     2041 ccgcgtatcc aggcggatta aattctcttc ctcttgttcg ccactggatt gctcggcaac
     2101 taatccgatc ccaagcccaa gagcaacata ggttttaatg acatcagaat cctgcgcact
     2161 taatacaata tctgccagca aacctttgcg ggcaaatgcg tcgtcaatac gtgagcgccc
     2221 cgtaatcccc tgtcggtaag tgattaacgg ccacttcgct attgattcca gcgtcaatgg
     2281 tgaaatttgc gtcaagggat gatcgtgtgg aacaagcaaa ctatggtgcc aacgaaacca
     2341 cgggaaggcg acgagctgcg ggtcattact caaacgctcg ctggcgatac caatatcagc
     2401 ttcgccattt tgcaacaatg tcgcaatttc ctgtggcgtc ccctggatta gctcgagccg
     2461 aacctccggg aaaagttcgc gaaaagcttt aatgacctct ggcaagctat aacgtgcctg
     2521 agtatgcgtc gttgcaatag tgagaacgcc agacgtatcg ttggtaaaca ggtctgcaag
     2581 ccgacgaaca ttactggctt cattcagaat acgttctgca atgaccagta atgctttgcc
     2641 cggttcagtc atgcccagca gtcgcttacc tcgtcgaaca aatatttcga tgccaagttc
     2701 atcctccagt tcccgaatat gacggctgac gcctgactgt gaggtaaaaa gcatattcgc
     2761 aacctctgtc aggttgtaat cctgacgtgc agcctcgcgg attatcttta gttgttggaa
     2821 attcacggta aactccgggc agttcagatt tcccgttatt gttaaagtct aatgcccggc
     2881 ataacaaata ataaaaaccc gcatcttatt ccatcccgat ataacactta gctcaccaat
     2941 tgccactgcc ttttttccat cactggagaa ctaatcactg acattaacaa ctctttcact
     3001 gcctgtgcct gtggcgataa gttcgctctg gcgggtaaat ttaatgacaa agagagactc
     3061 atggaaggag tggtaatgcg tgacatccac ccatttactg cgccacataa cgaacgcgcg
     3121 gccgattcgg gtaatactgc aacgcccatg ccgctggcaa tcgctgcggt aagcgtggca
     3181 atagactcaa tttcaccaat aacttttgcc gtgagtcgcc gtagggaaaa agcctcatca
     3241 acacgaagtc taatagcact gtaatcactg gggagaaaga ggttcatttg cgcaatagca
     3301 ttcacatcaa cgctttgccc cgggcaatct tgagttccta ccagaaaaag atcttctttc
     3361 agcaaagcct gactggatac accagccaca ggggaatgct cataaatcac cgccatatcg
     3421 agttggtgat ttatcaattt ttcgttaagc actgcaccac tattttcatg aagatagata
     3481 acgatctccg gaaattcagc gcgaaccgcc tgcaataagg gcatggtgat ggatgacgca
     3541 gcggttcctg gtgcaaagcc aatcgagact tgccccgata atgcctgacc aacgttatgc
     3601 accgccagtt gggcctgttc acactgacgt aaaatggccc gcgcatgggt atagagaatt
     3661 tttccggcgt ctgttggtgt aacgccccgc tttgtacgga tcaaaagttg ttgatttaac
     3721 tcaccttcca gtgtggcaac ctgctggctg agcgctggtt gtgcgatatg caatacttca
     3781 gcagcctggg tcaggctacc aatatctaca atttttacga agtatttcag gcgtctgaag
     3841 ttcatgttgc ctccggtttt taagaatcgg cccaagtgcc gccattactt acaaccagat
     3901 tgcaagatgc ttgccagttt tattttggtg ttgatgtaca agctaaccaa ctgtcaaata
     3961 agagattatg atagattcgt catttgctcc tttaatcagc tgtcgcgttc ccctgcccta
     4021 taaaaggagg gtatgcacca cgatggttca ttacccaata agattgaaag ctcaccactt
     4081 tgttgaaatt ggcagcaaac aaacaaaaaa atgcatttca ccctttgaca tcaccatgca
     4141 ctgccattaa tatgcgcccc gttcacacga ttcctctgta gttcagtcgg tagaacggcg
     4201 gactgttaat ccgtatgtca ctggttcgag tccagtcaga ggagccaaat tcaaaaaagc
     4261 ctgctttcta gcaggctttt tgctttctaa ttaccaacgc tcttaaaaca tctgtcttga
     4321 accagaacta atttgcacag gcattcccga tcgacgttgc aacgcagcat ttgcgcgatt
     4381 cacatcaact tcttgcccgt tgataaacgc ccgcaaagat ggggttaccg gcaatggcac
     4441 ttttcggtca gactcatatt ctgcacgatt gcgcgacagc ggctcatgaa cttccagcca
     4501 gttcgagcca tctggttcag tggtgtattt tactggctgg tcgataattt gcacacgcgt
     4561 tccaacagga acattatcaa acagatattt gatatcgtca ttgcgcagac gaatacagcc
     4621 ctgacttacc cgaagtccaa taccaaaatt ggcattggta ccgtggatgg catacaacct
     4681 gccaatataa atcgcgtaca gccccatggg attatcgggc cccgcaggaa caaatgcggg
     4741 caaactctcc cctcgtttcg catattcgcg ccgagtgttc ggcgttggcg tccaggttgg
     4801 agcttcttgt ttacgttcaa cggtagtcac ccagttacgc ggggtttctc gcccagcctg
     4861 gccgatacca ataggaaaga cttccacagt attactgtct ggcgggtagt aataaagacg
     4921 catctcagcg acgttaacca caatcccttt acgaacagtg gcgggcaaaa tcagttgctg
     4981 cggaatggtg agttgcgagc cagacttcgg caagaaaaca tcagcgcccg gattcgcttc
     5041 cagcatgtta cttaaccctt gcccgtattg tgcggcaaaa gtctccagcg gctgggtatt
     5101 gtgatcagga acagttacag taagcgactg ccccaccaaa cggctaccct ctggaggtaa
     5161 tggataagtt accgccaggc tagtatggct ggcaaaaagc agagcaaatg agcaaagaat
     5221 atttacacga cgcatcatgt ccctttccta tgtcgcgaaa gctatccgtt aagtatagct
     5281 tttatcagac ttttcgtttt taactgttca aatcagaagt cgtattcccc ggtagaacaa
     5341 tattactggc agcaagttcg cccatgttgt tatatatcgc acaggcagct tcgatgatgg
     5401 gcatcgccag agctgcgcca ctcccctcac ctaaacgcat ctccatattg agataaggct
     5461 ccagccccaa atgcgacagc gctatacgcg cccctttttc tgccgacagg tgagaaggaa
     5521 tgagatacgg tttgattgca ggagacatct ggcaggctgc gagcgcagca gcataagaaa
     5581 gaaatccatc cagcaacacg ggtaaaccgc aggaagcagc gcctaacatc acgccagcca
     5641 ttccgaccaa atcaaatccc cccacttttg ccagaacatc aacgccatcc tgcggatttg
     5701 gttgattcaa cgtaatcgcc cggcgcacaa catcaatttt attagccagt ttatctgtcg
     5761 gcaggtttgc gccaatccca accacttctt caggatcccg gccagtgatt gtgctgacta
     5821 ttgccgctgc cggtgtcgtg tttgccatcc ccagttcacc tacaccaaac agcgtgacac
     5881 cgttttttgc cagctcccgc gtataacata tgacgtccaa aagcaacttt tcagcctgac
     5941 ggcgactcat tgccggagct gaagcaatat tgccgctacc tcgtgcgaca cgcatgttga
     6001 taagcccggg gataggctca gcactatcaa taccaacatc aactacgtgg acgttagcgc
     6061 ccgcttgtgc tgccagcaca cacacgccgg ttgttccgcg ggtcatattt tcagcctgaa
     6121 tggctgtcac ttcttttggg gaaatagcga ccccttcctc ccagacgccg tgatcggcac
     6181 acataaccag taccgctttt ttgcccacat gcggtatgcc attcaacccc ggcattcccg
     6241 ccagttgtat ggcaagcgcc tccagccttc ccaggctacc aacaggtttg agtaacccgt
     6301 caatatgccg ttgtgcacgc gacatagcgg caggatcgat agcaggggtc gtattcagta
     6361 aatcggcaag tgtttgcatg tcacgtctct ttatagcagt gccagcagga agaccagttc
     6421 accaagctct atagcagctc ccagcgtatc gccggtttgc ccgcccagcg tacgtttgag
     6481 aagctgaccg aggatgaaaa tcgccaccat cgtcaccacc atagcagcca caccatgcat
     6541 acccggcaat aatacggcag caaaaatagc ggctaagccg agtgtgacgc aggtttgtcg
     6601 cccatcaatt ttgccaatga acacattgcc aagcccttct tcccgggcat aacgatgacg
     6661 atacatcaat aatgcggcag tcccgcggct gaccgcacat gctgccgcca gcgatgcaag
     6721 gatcggttcg ccacgcagag ccaactcgct taataccaga atctttgcca gtacaacaaa
     6781 aatcaatgcc agaccgccat gggtgcctaa acggctatca cgcatgattt ccaacatgcg
     6841 atcgcggcta cgtgcagaaa atacgccatc acaggtatcg gcgaggccgt ccagatggaa
     6901 tcccccggtc atcagcgcca gcacaagtac gctaaacagt gccgccagtg gtacgccaca
     6961 ccatgcctgc agcaccatga agaccagccc gctaatcgcg ccaagcaata atccaattaa
     7021 aggaaaagta ataataccgc gagaataatg ctcgaaatcc agtccctggg accagcgacg
     7081 cggtacaggc agacgcgtaa taaatgagag catcgcccaa aataatttac tcatttgatt
     7141 tttactccaa tacccgaaac caccagccat acttcatttg ccgcagcggc cagctgctga
     7201 tttacccgcc cggcaatatc acgaaaatgt cgtgccagac gactctccgg cacaatcccc
     7261 attcccactt cgttagtcac taatacaacc tttgcggggc aacgttggca ggcagcaatc
     7321 aacgactgaa tctcagcatt aatcgcctgt tccatcgcct gataatccca ttcatcaggg
     7381 tctttatcgc cgccataatc aaacaacaga ttagtcacca ttgtggtaac gcattccagc
     7441 aacacagctt catgcgggtt aatgtctgca tgaattaatt catcaagatg ttgccaacgc
     7501 tccaccgtgc gccagtgcgc cggacggcct tgccgatgat gttctatccg tgcagccatc
     7561 tcgtcatcaa ggatttgcga ggtagcgata tacagaacct gtgaagagtc cccaataagc
     7621 gcctctgcgt ggcgactctt cccgctccgt gcgccccccg tcaccaaaat catcataccg
     7681 actcctgatt caaatgtatg atggtattca ttgaacagaa caatggatcc cccaccgttg
     7741 ctgaccgcaa aacatttacg cgattaatac tgcgcgtaat ataattaaaa atccagcatt
     7801 ctcaatccat tacgaaataa gatggcatta cggaatagcc agactttctc gtgttaacgt
     7861 ccgacaaaat gcgtcattga cagcggtttc tgtggattga cacatttcat tcagataatg
     7921 aattaatgct actgccggac caaccagcaa acgggcattg tgttctgaaa tccaggctat
     7981 tgattcaacg tcagcgacag atctgcgctg taaaagaact tgtaaaacaa cgcaatagaa
     8041 ttatccggaa tggtggcgac tatgcactag aacaccacaa tttcgctctc tcggtaaggc
     8101 actgtcacag cattcaacag aatgtgactt gccagattta ttagcgccac cagatgttta
     8161 accgggtaac cacgacccag tcggggatat attccatctt ctcgctgaca tcatcaagaa
     8221 taaaaaggtt tatcacactc agagcaacgt tcttcagcag gcgggataat tcttcaacat
     8281 ttagaagatg tgttattaaa ggcatacaaa ctggacgtat tgtattttct tttggtggtt
     8341 gacctaaggt agcagtttat cctgatgcgc tgagatttct gtaatatcta cgtcaacatt
     8401 ctcctcgaat agaaaatgca gccccggcaa gtgattcaca ttttttaaca aaacgttgtt
     8461 agcgtgccat tttcagaagc tctccaagaa agcgaattct tttctcaaat tctgcattct
     8521 cagacacagc tttctactcc atggcaatgg cccaaatacg aagttgctca ggatcattag
     8581 tggtgctgag aagttaaata accatgcgga gtaccactcg tcatcacgta tttatatatc
     8641 atgaagctat tgttgtgttt tgtacatcag tagatatatt ctgatatact ccttttgcta
     8701 gacataacct ttcacctgct tgcaaagctt ctgtgttctg acattgccaa attgttgcaa
     8761 ttctgtatcc agccttcttt cagtcatagc ttcgggccgc gataagactc actgatctga
     8821 ccctgattcc tcttgcagac tttatagacc aattaaaatg cagtttctgc aggtcaacgt
     8881 ctgaccatca ttgtcatcac tctggccatt agagtaacct tctgcattca tccttttgta
     8941 aaaagtttat atcagtatca gcaattaacc ggacctgata ctgatatgag tcttaccgca
     9001 tatacggtta atttcagcaa ttaattacat tatccacgcc aaagtatttg tcatcacaat
     9061 gatggtacct tctttcagac accatttttt caactccgtt ttccacggac cgcactctta
     9121 tgtcaagagt gcggtccgtg gatacaacca gagaccgact gacacgagtc agaggaaacg
     9181 acggatatgt tcagtcgtaa aatatctatc aaaaaacatg attaaggtca aaaatgtttg
     9241 atatttacaa tttatgaaga tgacaataat tatagacaca tgagaacata aatgaaaata
     9301 attatcatta cagcaatctg aatcgccacg ggtttaacag acacctcaga gtcatttaag
     9361 atggcttaaa gagaggtgcc catgagcggt aagcgttatc ccgaagagtt taaaactgaa
     9421 gcagtcaaac aggttgttga tcgcggttat tctgttgcca gcgttgcaac acgtctcgat
     9481 atcaccaccc acagccttta tgcctggata aagaagtacg gtccggattc ttccactaat
     9541 aaagaacagt cagatgctca ggccgagatc cgccgtctcc agaaagagct gaaacgggtt
     9601 accgacgaac gggacatatt aaaaaaagcc a
//
LOCUS       DAPHDW010000160         9507 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.163, whole genome shotgun sequence.
ACCESSION   DAPHDW010000160 DAPHDW010000000
VERSION     DAPHDW010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9507
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.163"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..211)
                     /gene="cspA"
                     /locus_tag="RH057_003873"
     CDS             complement(<1..211)
                     /gene="cspA"
                     /locus_tag="RH057_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HDV3130173.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(492..782)
                     /locus_tag="RH057_003874"
     CDS             complement(492..782)
                     /locus_tag="RH057_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
                     /protein_id="HDV3130174.1"
                     /translation="MEYKDPMHELLSSLEQIVFKDETQKITLTHRTTSCTEIEQLRKG
                     TGLKIDDFARVLGVSVAMVKEWESRRVKPSSAELKLMRLIQANPALSKQLME"
     gene            1216..1926
                     /locus_tag="RH057_003875"
     CDS             1216..1926
                     /locus_tag="RH057_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HDV3130175.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQTDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(1976..2950)
                     /gene="ghrB"
                     /locus_tag="RH057_003876"
     CDS             complement(1976..2950)
                     /gene="ghrB"
                     /locus_tag="RH057_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HDV3130176.1"
                     /translation="MKPSVILYKALPDDLLQRLQEHFTVHQVANLSPQTVEQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRAHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(3054..3713)
                     /locus_tag="RH057_003877"
     CDS             complement(3054..3713)
                     /locus_tag="RH057_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HDV3130177.1"
                     /translation="MKKRVYLIAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSL
                     VGAGIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRS
                     GDNIILNMPNNVTFDSSSATLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDL
                     NMRLSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSP
                     L"
     gene            3866..6199
                     /gene="bisC"
                     /locus_tag="RH057_003878"
     CDS             3866..6199
                     /gene="bisC"
                     /locus_tag="RH057_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin sulfoxide reductase"
                     /protein_id="HDV3130178.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALDLIHQQHKRIR
                     EAYGPASIFAGSYGWRSNGVLHKASTLLQRYMALAGGYIGHLGDYSTGAAQAIMPYVV
                     GGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGLSYFSALRDSGKKL
                     ICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWHDEAFLTRCTTGYA
                     VFASYLLGESDGIAKNAEWAAEICGVNAAKIRELAALFHQNTTMLMAGWGMQRQQFGE
                     QKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGNPTRRSAVLSSMQGSLPGGCDAVDK
                     IPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQDTNRLIRAWQKPEL
                     VVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMKQVVPPRYEARNDF
                     DVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVELPPFAEFWQANQL
                     IEMSENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYGYPDCPGHPMWLEP
                     DEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVTIHPDDAQARGITE
                     GDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGICKNGAVNVLTKDL
                     PSSRLGNGCAGNTALAWLEKYNGPELTLTAFEPPASS"
     gene            complement(6168..6608)
                     /locus_tag="RH057_003879"
     CDS             complement(6168..6608)
                     /locus_tag="RH057_003879"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HDV3130179.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(6605..7168)
                     /gene="tag"
                     /locus_tag="RH057_003880"
     CDS             complement(6605..7168)
                     /gene="tag"
                     /locus_tag="RH057_003880"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HDV3130180.1"
                     /translation="MERCGWVSQDPLYIAYHDNEWGVPETDSKKLFEMICLEGQQAGL
                     SWITVLKKRENYRACFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFADFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYPGNKP"
     gene            7326..8024
                     /locus_tag="RH057_003881"
     CDS             7326..8024
                     /locus_tag="RH057_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3130181.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            8253..9455
                     /locus_tag="RH057_003882"
     CDS             8253..9455
                     /locus_tag="RH057_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3130182.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTWSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTMASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHI"
BASE COUNT         2227 a         2339 c         2483 g         2458 t
ORIGIN      
        1 acaggctggt tacgttacca gctgccgggc ctttagcgcc gctttcgatg gtgaaggaca
       61 ctttctgacc ttcgtccaga gatttgtaac catcgttctg gatagcagag aagtgtacga
      121 acacgtcttt agagccatcg tcaggagtga tgaagccgaa gcctttgtca gcgttgaacc
      181 attttacgat accagtcatt ttaccggaca tagtgtatta cctttaataa ttaagtgtgc
      241 ctttcggcga tatggcgtgc tttacagatt ttgaagcgtt aaaggaatgt gcactacgag
      301 gggtatcaac gataactctt gaagggactt gccttactac actgctttaa tggtctgtac
      361 gtcaaaccgt tgatgtgcat taagccacgc attggcgggt gatgcaacaa ttatttttca
      421 tatttatgat taatcggttg gaaaacggtc ctgtcatcag gaccgtaaac agcaataaag
      481 tggataaaag tctattccat caactgctta cttaatgccg ggttggcttg aatcaaacgc
      541 atcaatttta gttcggcact tgaaggcttc acgcgtctgg attcccattc ctttaccatg
      601 gcgactgata cgcccaaaac ccgggcgaaa tcatcgattt ttaaccctgt cccttttcgt
      661 aactgctcaa tttcggtaca ggacgttgtt ctgtgcgtca gggtaatttt ctgcgtttca
      721 tctttaaaaa caatctgttc caggctgctc aacagctcat gcattggatc tttatattcc
      781 attgaaaact cctcaaatca cactgcggga tcgtgaattg catcgaagct cattaagaat
      841 agtcgggaaa agcagacaca gcggggtgaa gtcagggcat gctctggcta tgattcgatt
      901 cagcctgtca tttagcgtga cttatagcca cctaacgatc tgataaatgc cgatatatct
      961 cttaccactc ctgttttgta aagatttcga aaatctaacc ttctgcgaag gcgtaaatct
     1021 ctggctacaa aaaaggcaac ggcactatca ttttttaaca atgaaacaac aaattaacct
     1081 tgtttcattt tctccatgat tttaccagga agattacgta gaaagggtat cctgcaagcc
     1141 tgtcctggac tatccttgtc acgtcagaca cgcgtgtgtc gttgtgcgct ttttttgggt
     1201 gaaaggagta agaaaatggc gacaggaaag tcctgctctc gctggtttgc gcctcttgcg
     1261 gcgttattaa tggtagttag cctgagtggg tgttttgata aagaaggcga tcagcgtaaa
     1321 gcgtttatcg acttcctgca gaatacagtg atgcgtagcg gtgaacgtct accaaccctg
     1381 actgccgatc agaaaaaaca gtttggtcct tttgtctctg attacgcgat tctgtatggt
     1441 tattctcagc aggtgaatca ggcgatggat tccggtctgc gcccggtcgt agacagcgtt
     1501 aacgcaattc gcgtaccgca ggattacgtt acgcaaagcg gtccgctgcg tgaaatgaac
     1561 ggttcgctgg gtgtgctggc gcaacaactg caaaatgcga agttgcaggc cgatgctgcg
     1621 cactctgcgc taaaacagac cgatgatttg aaaccggtct ttgatcaggc attcaccaaa
     1681 gtggtaacaa cgccagctga tgcattgcaa ccgttgattc cggctgcaca aactttcacg
     1741 caacaactgg tgatggttgg agactacatt gctcaacaag gtactcaggt gagctttgta
     1801 gcaaatggta tccagttccc gacttcgcag caggcaagtg aatataacaa actgattgcg
     1861 ccgttaccgg cacaacatca ggcgtttaat caggcctgga ctacagcagt tactgcaacc
     1921 caataaagag taaaagcccg agcgattatc gggctttact tcacgcagtc gcggcttagt
     1981 ccgcgacgtg cggattcaca cagttcttct caacctttcc ttgtaacgca tcaatcaaat
     2041 tatccacggc acaggcggcc atgccataac gcgtctcatg ggtggcagat ccaatatgcg
     2101 gtactgcgac gacgttggcc attgagagca acggcgaatc tacggacagt ggctcttgtt
     2161 cgaagacatc cagcccggcg gcgtgaattt cgcctttctg caatgcagca atcagtgcat
     2221 tttcatcaac caccgggcca cgtccggcat taatgaaaat ggcggaggat ttcattttgg
     2281 cgaattgttc tgcgccaaac agatgatgcg tctcatcagt taacggcagg atcaggcaaa
     2341 cgaaatctga ctcttgtaac agagtatcca aatcgcagta gcgggcgttg aagcgttctt
     2401 ctgcttcttt atggtggcgg cgcgcgttat agaggatggg catgttgaag ccaaagtgcg
     2461 cacgttgtgc cagcgccatg ccgatccgtc ccatcccgac aatgcccagt gttttatggt
     2521 gaacgtcagt gccgtaccag tccgggccta tgctcgcggt ccattcgcct gcttttaccc
     2581 gttctgctac ctccacaacc cgacgagcgg tagacaacac cagcgccatc agcgtatcgg
     2641 cgacggtttc tgttaatacg gttggcgtgt gcatcagcag aatttttcgg gcggtaagcg
     2701 catcgacatc aaaattgtca tagccgacgg agatcgttga tgtggcacgc agtttcggca
     2761 ttttttccag caatgcggca tctacattct cgtttgaacc cagtaaacct tcagcttcgg
     2821 caaaaattgc tgcattttgt tcgacggttt gtgggctgag gtttgccacc tggtgaacgg
     2881 tgaaatgctc ttgcaggcgt tgcagtaaat catcaggtaa ggctttgtag aggataacgg
     2941 acggcttcat gcttctctcc attcactgat aaggcgatca ccttagcctg aaatatgatg
     3001 aaggaaaact gaagaatgcc aggtgatttc tgcatcacct ggcatgaaag ggattacagc
     3061 gggcttaagg taatttctac acggcggttt tgcgccttac cttctgcggt gctgttgctg
     3121 gcgattgggt tagccgggcc aaggccctga gtacggatgc ggctggcgtc cacgccctgg
     3181 gtgatcaacg cgctggcaac ggaatccgca cgttgctggg agagacgcat gttcaggtcg
     3241 tgaccacccg tgctgtcggt ataaccaatc acgttaaccg ccgttttcgg atactctttc
     3301 agcaccattg ccacgccggt cagggtgtta gcgcctgccg gtttcagggt cgcgctgctg
     3361 ctgtcgaagg tcacattgtt cggcatattg aggataatgt tatccccgct gcgggttacg
     3421 ctaacaccag tgccgcgcat tttgtcacgc agcttcgctt cctgcacatc catgtaataa
     3481 cccacgccgc cgcccagagc tgcgcctgct gctgcgccaa tcagcgcgcc tttaccgcga
     3541 tctttcttcg aagaagagag cgcaccaata cccgcgccca cgagagagcc cagacctgcg
     3601 ccgatagcag atttacctgc ttcgcgttcg ccggtgtaag ggttagttgt gcagccagat
     3661 accgccagag cgccactcac tacggcggca ataagataaa cacgtttctt cattgttaat
     3721 ccttaataac ctttttattc tttgccacgg gttccgtggc gggagattat gccgcgtgaa
     3781 catgaagatt attcctggga atactcggaa atttgtaagt aatatttaac tgctcaatac
     3841 atctaacctt tcaggagtct tcggtttggc caactcatcc tcacgatatt ccgttctgac
     3901 tgccgcccac tgggggccca tgctggttga aaccgacggc gaaaccgtgt ttagctcgcg
     3961 tggcgcgtta gccacaggaa tggaaaactc cttgcagagc gcggttcgcg accaggttca
     4021 cagcaatacg cgggtacgat ttccaatggt gcgcaaaggc tttctcgcgt caccggaaaa
     4081 cccgcaaggc attcgtgggc aggatgaatt tgttcgcgtg agttgggatg aggcgctgga
     4141 tcttattcac caacaacata aacgcattcg tgaggcttat ggtccggcat cgatttttgc
     4201 tggttcctac ggctggcgtt caaacggcgt gctgcataag gcctcgacat tattacaacg
     4261 ctatatggcg ctggcaggcg gttatatcgg gcatctgggg gattattcga ccggtgcggc
     4321 acaggcgatc atgccgtatg tcgtgggtgg cagcgaagtt tatcaacagc aaaccagttg
     4381 gccgctggtg ctggaacata gcgatgtcgt ggtgttgtgg agtgctaacc cactcaatac
     4441 gctgaaaatt gcgtggaatg catctgatga gcaggggctt tcttactttt ccgcactgcg
     4501 tgacagcggg aaaaagctga tctgtattga tccaatgcga tcggaaaccg tcgatttctt
     4561 tggcgataaa atggagtggg tggcaccgca catgggcacc gatgttgcgc tgatgctggg
     4621 gatcgcccat acgctggtgg aaaatggttg gcacgacgaa gcgtttctga cgcgttgcac
     4681 cacaggttat gccgtcttcg cctcttattt gctgggcgag agtgacggaa tagcgaaaaa
     4741 cgccgaatgg gcggcagaga tttgtggggt taacgcagcg aaaatccgcg agctggcggc
     4801 tcttttccac caaaacacga ccatgctgat ggctggctgg ggaatgcagc gccaacagtt
     4861 tggtgagcaa aaacactgga tgatcgtcac gctggcggca atgttggggc aaatcggcac
     4921 acccggcggc ggttttggtc tttcttatca ttttgccaat ggtggtaacc ccacgcggcg
     4981 ttctgcggtg ctctcttcca tgcagggcag cttgccgggt ggctgcgatg cggtggataa
     5041 aatccctgtt gcccgcattg ttgaagcact ggaaaaccct ggtggcgcat atcaacacaa
     5101 cggtatgaac cgacatttcc cggatattcg ttttatctgg tgggcgggcg gtgccaactt
     5161 tactcatcat caggatacca atcgtctgat ccgtgcctgg caaaaaccgg agctggtggt
     5221 gatctctgaa tgcttctgga cggcggcggc gaaacacgcg gatatcgttc tgcctgcgac
     5281 tacctctttt gagcgtaatg atctcaccat gaccggtgat tacagtaatc agcatctggt
     5341 gccgatgaag caagtggtgc cgccacgcta tgaagcgcgt aatgattttg atgtttttgc
     5401 cgagttgagt gaacgttggg agaagggcgg ctatgcgcgg tttacggaag gaaaaagtca
     5461 gctgcaatgg ctggaaacgt tttataacgt tgcccggcag cgcggggcaa gccagcaggt
     5521 tgaattgccg ccatttgctg agttctggca agccaaccag ttaattgaga tgtcggaaaa
     5581 cccggacagc gagcggttta ttcgcttcgc tgatttttgc cgcgatccgc tggcgcatcc
     5641 gttaaaaacc gccagcggca agattgaaat cttctcacag cgtattgccg attacggtta
     5701 cccggattgc cctgggcatc caatgtggct ggagccggac gaatggcagg gcaatgccga
     5761 accagaacag ttgcaggtac tttctgccca tccggcgcac cgcctgcaca gccagctgaa
     5821 ttacagttct ctgcgcgaat tgtacgcggt agcaaaccgt gagcctgtca ccattcatcc
     5881 tgacgatgct caggcgcgcg gcataaccga aggggatatg gtgcgggtgt ggaactcccg
     5941 tgggcagatc cttgccggag cggtcattag cgagggaatt aaacctggcg tgatttgcat
     6001 tcacgaaggg gcatggccgg atctggattt aaccgctgac ggtatttgta aaaacggcgc
     6061 agtgaacgtg ctgaccaaag atctccccag ctcgcggctg gggaatggct gtgcgggtaa
     6121 tacggcgctg gcatggctgg aaaaatacaa cggtccggaa ctgacactta cagcgtttga
     6181 accaccggcc agctcataat ccaggtgggt agttgggttt catcctgcca tgcgcaatcg
     6241 acaatgtgaa aaccctgcgc acggtaaaaa tctatcgccg gttgattttt ttgataaacc
     6301 tccagcatca gatggggata gcgctgctgc acatactgca tcagcgcctt accaataccg
     6361 cgtctgacgg ccttcggtgc gacaaacatt gctgccagaa atcggccttc cataatgctg
     6421 acaaaaccga gaagcttacc gtcctcttcc cagacccagt tttgcgcgtt ggcaagatag
     6481 gcatcccgca ccagcggaat gcagtcacgc cagtaattcg cttttataaa gggatgcccc
     6541 caggttgtac tttccagcca cagttcgagg atcgcgggga gttctgaacg ttgcgcttcc
     6601 cgaatcatgg tttatttccc ggatagcaac agcagccaac cacatgatca ttcaccagcc
     6661 cacatgcctg cataaaggag taacagattg tggtgccgac aaacttaaaa ccacgttttt
     6721 tcagtgcctt agatagggcg tcggaggcgg acgtagatgt gggaatttcg ctcaacgttg
     6781 tggcttgtgt cacctgtggc tgatgattta caaacgacca gacaaagtcg gcaaacggtt
     6841 cgccgttctg ttccatttgc aggtacgccc gcgcattacc aataattgcc tgaattttcc
     6901 ctcgatggcg gataatcccg gcgtcctgta ccagtctttc gacatcctct tcctgcattg
     6961 ctgcgacctt caccggatcg aactgatgaa agcaggcgcg atagttttcg cgttttttga
     7021 ggacggtgat ccacgataac ccagcctgct gcccttcaag gcagatcatt tcgaacagtt
     7081 ttttactgtc agtttcaggc acgccccatt cattatcatg gtaggcaata taaagcgggt
     7141 cctgactcac ccagccgcaa cgttccatac tttccctcgc tatgtacccg atcgtaaaaa
     7201 tttcagccgc ttttcttgac gcatcggtga aaaagtcaat atttaataca gggctactat
     7261 gcccgatatc cttcatacaa tgacaataat attgccgaat tcggctcttc cctggagtcg
     7321 tgttaatgat cataaaaaaa agcggtggtc gctggcagct aagcctgctg gcgagcgtgg
     7381 taatcagtgc cttttttctc aacacagctt acgcctggca acaagaatat atcgttgata
     7441 cgcaacccgg gcattccaca gagcgttaca cctgggatag tgatcatcaa cctgattaca
     7501 acgatatttt gtcgcaacgt attcaaagta gccaaagggc gctgggactg gaagtcaatc
     7561 tggcggaaga aactcctgtg gatgtgacca gcagtatgag tatgggctgg aattttcctt
     7621 tatatgaaca ggttacaacc ggcccggtcg cggcattaca ttacgatggc acaaccacct
     7681 cgatgtataa cgagtttggc gacagtacta ccacgctgac cgatccgtta tggcatgcca
     7741 gcgtgagtag cttaggctgg cgtgttgact cccggcttgg cgatctccga ccctgggcgc
     7801 aaatcagcta taaccagcaa tttggcgaga atatctggaa ggcgcaatca ggcctgagcc
     7861 ggatgacggc gacaaaccag aacggcaact ggctggatgt caccgtaggc gctgatatgt
     7921 tgctcaatca aaatattgcc gcctatgccg cgctaactca ggcagaaaat accactaata
     7981 atagcgacta tctgtatacg atgggggtta gcgccagatt ttaacgtaac agtcacaatt
     8041 gaaaccattg aataacaaca gttgtggcga tagtgggtgc taacttacca aataataaat
     8101 ttggtgaata attgtcgcgt cattcattcc tgaactaagg catttcattc cgttctgatg
     8161 gcatttcatg ccgtttttcc ccaggcataa agtgcacttc gttatggttg tcggcagaga
     8221 tttttccttt ttattactgc aggaatactg ccatgacacc ttcaaattat cagcgtaccc
     8281 gctggctgac actcatcggt acaatcatta cccagtttgc gctggggtcg gtttatacct
     8341 ggagcctgtt taatggcgcg ctttccgcca agctggatgc gccggtaagc caggtcgctt
     8401 tctctttcgg cttgttaagt ctggggctgg caatttcgtc ttctgttgcg ggcaaattgc
     8461 aggaacgttt tggcgttaaa cgcgtcacca tggcttccgg cattttgctg ggattaggct
     8521 tcttcctgac cgcgcattcc aacaacctga tgatgctgtg gttaagcgcc ggtgtgctgg
     8581 tcggtctggc ggatggcgcg ggttacctgc tgacgctctc taactgcgtg aagtggttcc
     8641 cggagcgtaa aggtctgatc tccgcgttcg ctatcggttc ttatggtctg ggtagcctgg
     8701 gtttcaaatt tatcgacacg cagctgctcg aaacggtggg tttggaaaaa acatttgtga
     8761 tttggggagc gattgcgctg gtgatgattg ttttcggcgc aacgttaatg aaagacgcac
     8821 caaaacagga agtgaaaacc agcaatggtg tggtggagaa agattacacg ctggcagagt
     8881 cgatgcgtaa accgcagtac tggatgttag cggtaatgtt cctgactgcc tgcatgagcg
     8941 gcctgtacgt gattggggta gcgaaagata tcgcccaaag tctggcacac cttgatgtgg
     9001 tttccgcagc caatgcagtc actgttattt ccatcgctaa cctttcaggt cgtctggtgc
     9061 tgggcattct gtctgacaaa attgcccgta tccgtgttat caccattggt caggtgattt
     9121 cgctggtggg tatggcggcc ctgctgtttg caccattaaa tgcagtgacg ttctttgcag
     9181 cgattgcctg cgtggcattt aactttggcg gcactattac cgtctttcca tcactggtca
     9241 gtgagttctt cggcctcaat aacctggcga aaaactacgg tgtgatttac ctcggtttcg
     9301 gtatcggtag catttttggt tcgattatcg cctcactgtt tggcggtttc tatgtgacct
     9361 tctacgtgat tttcgccctg ctgattctgt cattggcgct ttctacgacg attcgtcagc
     9421 cagagcagaa aatgttgcgt gaggcgcata tttagaaata tgttgctact gaaataaata
     9481 cgagtaggga gacttcaaag tctccct
//
LOCUS       DAPHDW010000161         9263 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.164, whole genome shotgun sequence.
ACCESSION   DAPHDW010000161 DAPHDW010000000
VERSION     DAPHDW010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9263
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.164"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            42..1385
                     /locus_tag="RH057_003883"
     CDS             42..1385
                     /locus_tag="RH057_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310521.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3130183.1"
                     /translation="MVSMGTQKLKAQSFFIFSLLLTLILFCITTLYNENTNVKLIPQM
                     NYLMVVVALFFLNAVIFLFMLMKYFTNKQILPTLILSLAFLSGLIYLVETIVIIHKPI
                     NGSTLIQTKSNDVSIFYIFRQLSFICLTSLALFCYGKDNILDNNKKKTGILLLALIPF
                     LVFPLLAHNLSSYNADYSLYVVDYCPDNHTATWGINYTKILVCLWAFLLFFIIMRTRL
                     ASELWPLIALLCLASLCCNLLLLTLDEYNYTIWYISRGIEVSSKLFVVSFLIYNIFQE
                     LQLSSKLAVHDVLTNIYNRRYFFNSVESLLSRPVVKDFCVMLVDINQFKRINAQWGHR
                     VGDKVLVSIVDIIQQSIRPDDILARLEGEVFGLLFTELNSAQAKIIAERMRKNVELLT
                     GFSNRYDVPEQMTISIGTVFSTGDTRNISLVMTEADKALREAKSEGGNKVIIHHI"
     gene            1566..3056
                     /locus_tag="RH057_003884"
     CDS             1566..3056
                     /locus_tag="RH057_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3130184.1"
                     /translation="MKLHHRMLRHFIAASVIVLTSSFLIFELVASDRAMSAYLRYIVQ
                     KADSSFLYDKYQNQSIAAHVMRALAAEQSEVSPEQRRAICEAFESANNTHGLNLTAHK
                     YPGLRGTLQTASTDCDTIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDL
                     NDRYVYFYEPVNVEYFAMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQN
                     VMSLLTPVYVAGQLKGIVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIII
                     NQSEDNLFQYVSYVHDLPGGIRVSLSIDILYFITSSWKSVLFWILTALILLNMVRMHF
                     RLYQNVSRENISDAMTGLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKLINDT
                     LGHQEGDLAITLLAQAIKQSIRKSDYAIRLGGDEFCIILVDSTPQIAAQLPERIEKRL
                     QHIAPQKEIGFSSGIYAMKENDTLHDAYKASDERLYVNKQNKNSRS"
     gene            3099..3602
                     /gene="yeaK"
                     /locus_tag="RH057_003885"
     CDS             3099..3602
                     /gene="yeaK"
                     /locus_tag="RH057_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HDV3130185.1"
                     /translation="MTEMAKGSVTHQRLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTTDYLRIAQPEL
                     LNFRRTA"
     gene            complement(3603..3707)
                     /locus_tag="RH057_003886"
     CDS             complement(3603..3707)
                     /locus_tag="RH057_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130186.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERTG"
     gene            3877..4323
                     /locus_tag="RH057_003887"
     CDS             3877..4323
                     /locus_tag="RH057_003887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HDV3130187.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(4280..5101)
                     /gene="nimR"
                     /locus_tag="RH057_003888"
     CDS             complement(4280..5101)
                     /gene="nimR"
                     /locus_tag="RH057_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
                     /protein_id="HDV3130188.1"
                     /translation="MMHRLNLNGYEPDRHHEAAVAFCIHAGTDELTSPVHQHRKGQLI
                     LALHGAITCTVENALWMVPPQYAVWIPGGVEHSNQVTANAELCFLFIEPSAVTMPTTC
                     CTLKISPLCRELILTLANRTTTQRAEPMTRRLIQVLFDELPQQPQQQLHLPVSSHPKI
                     RTMVEMMAKEPVEWGSLGQWAGFFAMSERNLARLIVKETGLSFRQWRQQLQLIMALQG
                     LVKGDTVQKVAHTLGYDSTTAFITMFKKGLGQTPGRYIAGLTTVSPQSAKPDPRQ"
     gene            5198..6379
                     /gene="nimT"
                     /locus_tag="RH057_003889"
     CDS             5198..6379
                     /gene="nimT"
                     /locus_tag="RH057_003889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
                     /protein_id="HDV3130189.1"
                     /translation="MTCSTSLSGKNRIVLIAGILMIATTLRVTFTGAAPLLDTIRSAY
                     SLTTAQTGLLTTLPLLAFALISPLAAPVARRFGMERSLFAALLLICAGIAIRSLPSPY
                     LLFGGTAVIGGGIALGNVLLPGLIKRDFPHSVARLTGAYSLTMGAAAALGSAMVVPLA
                     LNGFGWQGALFMLMCFPLLALFLWLPQWRSQQHANLSTSRALHTRGIWRSPLAWQVTL
                     FLGINSLVYYVIIGWLPAILISRGYSEAQAGSLHGLLQLATAAPGLLIPLFLHHVKDQ
                     RGIAAFVALMCAVGAVGLCFMPAHAITWTLLFGFGSGATMILGLTFIGLRASSAHQAA
                     ALSGMAQSVGYLLAACGPPLMGKIHDANGDWSVPLMGVAILSLLMAIFGLCAGRDKEI
                     R"
     gene            6434..6781
                     /locus_tag="RH057_003890"
     CDS             6434..6781
                     /locus_tag="RH057_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HDV3130190.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYLAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(6803..7057)
                     /gene="yoaF"
                     /locus_tag="RH057_003891"
     CDS             complement(6803..7057)
                     /gene="yoaF"
                     /locus_tag="RH057_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HDV3130191.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            7240..8265
                     /gene="dgcP"
                     /locus_tag="RH057_003892"
     CDS             7240..8265
                     /gene="dgcP"
                     /locus_tag="RH057_003892"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HDV3130192.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVSWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLREANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            8298..8396
                     /locus_tag="RH057_003893"
     CDS             8298..8396
                     /locus_tag="RH057_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HDV3130193.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGGYAAPGA"
     gene            8399..8473
                     /gene="yoaJ"
                     /locus_tag="RH057_003894"
     CDS             8399..8473
                     /gene="yoaJ"
                     /locus_tag="RH057_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HDV3130194.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(8532..8780)
                     /locus_tag="RH057_003895"
     CDS             complement(8532..8780)
                     /locus_tag="RH057_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HDV3130195.1"
                     /translation="MGILSWIIFGLIAGILAKWIMPGKDGGGFFMTILLGIVGAVVGG
                     WISTLFGFGKVDGFNFGSFVVAVIGAIVVLFIYRKIKS"
     gene            complement(9007..>9263)
                     /locus_tag="RH057_003896"
     CDS             complement(9007..>9263)
                     /locus_tag="RH057_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130196.1"
                     /translation="GGQVLAVPPAYTSQRCACCGHTAKENRLSQSQFRCQVCGYTANA
                     DVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         2304 a         2227 c         2148 g         2584 t
ORIGIN      
        1 tgccatcaat aaaatccagc actcacattg ctctcctttt tatggtttct atgggtacac
       61 aaaaattaaa agctcaaagc ttttttattt tcagtttatt gctgacgtta attttatttt
      121 gcatcaccac attatataac gaaaacacaa atgtaaaact catcccacag atgaattacc
      181 tgatggttgt tgtggctttg tttttcctta acgccgtcat ttttcttttc atgttaatga
      241 aatatttcac taacaaacaa attttaccaa cactcatttt aagccttgca tttttaagtg
      301 gccttatcta tttagttgaa accattgtaa ttatccataa accaattaac ggcagtacac
      361 tgatccagac aaagtcgaat gatgtttcta ttttctatat tttccgccaa ctcagtttta
      421 tttgtttaac ctcgctggca ctcttttgtt atggaaaaga caacatcctt gacaacaata
      481 agaaaaaaac gggaatcctg ttgctggcgc tgattccttt tttagttttt ccccttctgg
      541 cacacaatct gagcagttat aacgctgact attctttgta tgtcgtcgac tactgtccgg
      601 acaaccatac tgcgacctgg ggaatcaact atacaaaaat attggtttgt ctgtgggcat
      661 ttttactgtt ctttattatc atgcgtacac gattagccag cgaactatgg ccgttaatag
      721 cattattatg tctggcatcg ctatgctgca acttacttct actgactctg gatgagtata
      781 attacaccat ctggtatatc agtcgcggga ttgaagtttc cagtaaactg tttgttgtgt
      841 cttttctgat ttataacatt tttcaggaac tgcaactctc cagcaaactg gcagttcatg
      901 atgtgctgac caatatttat aatcggcgct actttttcaa cagcgtagag tcattattgt
      961 cgcgacctgt tgttaaggac ttctgtgtca tgctggttga tattaatcag ttcaaacgca
     1021 tcaatgccca atggggacat cgtgtgggtg ataaagtgct ggtttcaatt gtcgatatta
     1081 tccagcaaag catccgccct gatgatattt tagcgcgact ggagggtgag gtgtttggct
     1141 tgctatttac cgaactcaat agtgcccagg caaaaatcat tgcggaacgt atgcgtaaaa
     1201 atgtcgaact cctgaccggc tttagtaaca gatatgatgt tcctgaacaa atgaccatca
     1261 gtattggcac ggttttttca acgggtgaca cgcgtaatat ctcgcttgtc atgacggaag
     1321 cagataaagc cttacgcgaa gcgaaaagcg aggggggcaa caaagtgatt attcatcata
     1381 tttaagtgca aaaatattca gagccatgct ttttgcgtgg cttttgcata caatttatta
     1441 ctattacccc taaatttccc ctcccctgcg gcgcagagat gagtataatt agcgcccctg
     1501 tgccaggccg caatcgaact ttatctggtt ttctcgtttc actaaccgaa ggagtgccat
     1561 ttatcatgaa attgcaccat agaatgctcc ggcattttat cgccgcaagt gtcattgtgc
     1621 tgacatcttc cttccttatt tttgaacttg tcgccagcga cagagcaatg agtgcctatc
     1681 tgcgctatat cgtgcagaaa gcagactcct cctttcttta tgataagtat cagaatcaga
     1741 gtattgccgc gcatgtgatg cgcgctctcg ctgctgagca gtcggaagtg tcgccagaac
     1801 agcggcgcgc catctgcgag gcttttgagt ctgccaataa tacccatggc ttaaacctga
     1861 ctgcccataa atacccgggc ttacgcggca cactacaaac cgcatccact gactgcgaca
     1921 caattgtgga agctgcagca ctattacccg cttttgatca ggcagtggaa ggcaaccgcc
     1981 accaggatga ttacggttca ggtcttggga tggccgaaga gaaatttcac tattatctcg
     2041 atctcaatga ccgctatgtc tatttttatg agccggttaa tgttgaatac tttgcgatga
     2101 ataactggtc cttcctgcag tcaggaagta ttggtatcga tcgcaaagat attgaaaagg
     2161 tatttaccgg gcgtaccgta ttgtcgagca tttaccagga tcagcgtact aaacagaacg
     2221 tgatgagttt gctgacgccg gtgtatgttg cagggcagct aaaagggatt gtgctgctgg
     2281 atattaacaa aaacaatttg cggaatatct tttacactca tgaccgccct ctcctctggc
     2341 gttttctcaa tgtcacgcta accgataccg attcggggcg cgacattatc atcaaccaga
     2401 gcgaagataa tctgttccag tatgtcagtt acgtccatga cttaccgggc ggcattcgtg
     2461 tctcgttatc cattgatatt ctttacttta tcacgtcttc gtggaaaagc gttctgttct
     2521 ggattttgac tgcgttaatt ttgctgaata tggtccggat gcacttccgt ttataccaaa
     2581 atgtgtcgcg agaaaatatt agtgatgcga tgactggact gtataatcgc aaaattttaa
     2641 cccctgaact ggaacagcgg ttgcagaaac tggtgcaatc cggttcttcg gtgatgttta
     2701 ttgcaattga catggacaag ttaaagctaa taaatgacac cctcggtcat caggaagggg
     2761 atttagcgat tacgttgtta gctcaggcga ttaaacaatc gattcgtaaa agtgattatg
     2821 ccatccgact cggtggcgat gaattctgca tcattcttgt cgattcgacg ccgcaaattg
     2881 cagcacaact gcctgaacgt atcgaaaaac gtctgcaaca tatcgcgccg cagaaagaaa
     2941 tcggcttctc ttccggtatt tacgcgatga aagaaaacga tacgttacat gatgcgtata
     3001 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     3061 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     3121 gcgtgacgca tcagcggtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     3181 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggc accgctttag
     3241 gccagggagc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     3301 tggcgatcct cgctgccgat caacaggccg atctgagcca acttgccagc catataggcg
     3361 gattacgcgc ttcgttggcc agcccggcgg aagtcgatga actcacaggc tgtgtcttcg
     3421 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     3481 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     3541 aaaccacgga ttatttgcgc attgcgcaac cagaactgct caatttccgc cgcactgcgt
     3601 aactagcctg tccgttcaat aagcagaacg gacaaaacca gcacaacagc aatggcaaaa
     3661 aacgatgacg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     3721 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     3781 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcgtaaag
     3841 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     3901 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     3961 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     4021 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     4081 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     4141 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacctt aatgggcagc
     4201 cagccgcaac tggtcgccgg gctgctggta ggcactgttt taggcgtggc gctgtttcgc
     4261 ggcgtaccgg tcggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     4321 tagttaatcc cgcaatatat cggcctggcg tttgccccag gcctttctta aacatagtaa
     4381 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     4441 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     4501 accccgtctc tttcactatc agccgggcaa gattgcgttc actcatcgca aaaaagccag
     4561 cccattgccc caatgagccc cactcgacgg gctctttcgc catcatctcc accatcgtgc
     4621 ggattttagg atgagaagaa acgggcaagt gcagctgttg ttgtggctgc tgagggagtt
     4681 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     4741 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     4801 ttgtcggcat tgtcactgcg ctgggttcga taaacagaaa acagagttca gcgttagctg
     4861 tcacttgatt gctatgttcc accccgcccg gtatccaaac cgcatattgc ggcggcacca
     4921 tccacaaagc attttccacc gtacaggtaa ttgcaccatg taatgctagt atcagctgtc
     4981 ctttgcggtg ctgatgtaca ggactggtta actcatccgt tccggcgtga atacaaaatg
     5041 cgaccgcggc ttcatggtgt cggtcgggtt catagccatt gagattcaac ctgtgcatca
     5101 ttttgtccga atttagcgat aatttgtcat tttagcttga ttcaacataa caataaaaac
     5161 ggtaaggtac agcctcgttt gtaacaatga gaagcatatg acctgttcaa cttcattaag
     5221 cggcaaaaac aggattgtcc ttatcgctgg cattctgatg attgccacta cattacgcgt
     5281 caccttcacc ggcgcagcac cgttactcga tacgattcgt tccgcttact cgctgacgac
     5341 agcgcaaacc ggcttattaa ccaccctgcc attattggcc tttgcgctaa tctcaccttt
     5401 ggctgccccg gtggcgcgac gttttggtat ggaacgtagc ctgtttgccg cgttactttt
     5461 gatctgtgca ggtatcgcaa ttcgttctct cccttcgcct tacttattat ttggcggtac
     5521 agcggtcatt ggcggtggga ttgcattagg caatgtctta ctgccaggat taattaaacg
     5581 cgatttccct cattccgtcg ccagacttac cggcgcatat tccctgacaa tgggagctgc
     5641 agcggcactg ggatcggcta tggtcgtgcc gctggctttg aacggttttg gctggcaagg
     5701 cgcgttgttc atgctgatgt gttttcctct gctggctctt tttttatggc tgccacagtg
     5761 gcgaagccaa caacatgcaa atttgagtac ctcgcgcgcc ttacatactc ggggtatctg
     5821 gcgttcgccg cttgcctggc aggtcacatt gtttcttggg atcaactcac tggtctatta
     5881 cgtgattatt ggctggcttc cggcgatcct catcagtcgc ggctatagcg aagcacaggc
     5941 gggttcactg catggtttgc tgcaactagc cacagcagct cccggtttgc tgatccctct
     6001 tttcttacat catgtgaaag atcagcgtgg tattgcagcg ttcgttgcct tgatgtgcgc
     6061 agtgggcgct gttgggctct gctttatgcc agcgcacgcg atcacctgga ctctgctttt
     6121 cggttttggt tccggcgcaa caatgatact ggggttgacg ttcattggtc ttcgggcaag
     6181 ttctgcgcat caggcggcgg cactctcggg gatggcacaa tccgtcgggt atttgctggc
     6241 agcctgtggg ccgccgctga tgggtaaaat acacgatgct aacggtgact ggtccgtacc
     6301 actgatgggt gttgccatac tttcactact gatggcgatt ttcggacttt gcgccgggag
     6361 agacaaagaa attcgctaat atccggtgct atagtgacgt gacaaatcat gcgtgaaagg
     6421 gagaacaaac acgatgaata ttcagtgcaa acgcgtttat gatccggctg aacagagcga
     6481 tggttatcgc gtactggtcg accgcctctg gccgcgcggt atcaaaaaaa ccgatttagc
     6541 ccttgatgag tgggataaag aaatcacgcc gtcaacggaa ctgcgcaaag cctttcacgg
     6601 cgaagtcgtc gattttgcaa cctttcgcga gcaatatctt gcagaactgg cgcaacacga
     6661 gcaagaagga aagcggctgg cggacatcgc caaaaaacag ccgctgaccc tgctctacgc
     6721 agcaaaaaac accacgcaga accatgcgct ggtgctggcc gactggctac gtagcttgtg
     6781 attttagtac agcatccggc ggttattttt caccagccgg atggtcccgt cgccacaatg
     6841 cccattcatc aatcgtttca ccgcccggta atttgcaatt gttgctgacc ccttgcgctg
     6901 tctgcactgg aatgagcgtc ccgcccttct gctggcaata gaccgacgcc ggatttgcca
     6961 taccaatctg cggcggttta ggtgcttctg gctgagaagg ggttgaacaa ccagccagga
     7021 ccagcaagca aggcagaaca aaactgataa ttttcattta ttgatctcac atatttatcc
     7081 agacttagag tatcgcggta tcgttttgtt ttgcagcact atttttatta cattcactca
     7141 aatcatatta cgccttgttt catctttgtt gatgatgttt tatcatgcct gcaaagatta
     7201 aataatcagc atttacccgc cgtatcctgg agttgtttcg tgtcagatca gattatcgcc
     7261 cgcgtctcgc aatcccttgc caaagaacag tcactggaaa gtctggtccg acagcttctg
     7321 gagatgctgg aaatggtcac tgatatggaa tcaacctacc tgaccaaagt ggatgtcgaa
     7381 gcgcgcctgc agcatattat gtttgcccgt aacagccaga aaatgcacat cccggagaat
     7441 tttaccgtct cgtgggatta ctcgttatgc aaacgcgcta ttgatgaaaa ctgctttttc
     7501 agcgatgaag tccctgaccg ttggggcgac tgtattgcgg cacgcaatct tggaatcacc
     7561 acatttctga gtacaccaat tcacttaccg gatggatcat tctatggcac gctttgcgcc
     7621 gccagcagtg agaagcgcca gtggagtgaa cgcgcggaac aggttttaca gttattcgcc
     7681 ggactgattg cacaatatat tcaaaaagag gcgctggttg aacagctgcg cgaagccaat
     7741 gccgcgctga ttgcgcaatc gtataccgac tcgttaaccg ggctaccgaa tcggcgggcg
     7801 atttttgaaa atctgacgac gctgttttct ctcgcccggc atcttaacca taagataatg
     7861 atcgcgttta tcgatctgga taacttcaaa ttaatcaatg accgttttgg tcataatagt
     7921 ggcgatctgt ttctcattca ggttggcgag cgccttaata cgctccagca aaatggcgaa
     7981 gttattggtc gtctcggcgg tgatgagttt ttggttgttt cactaaacaa cgagaatgcg
     8041 gatatttcgt cgctgcgaga acgtattcaa cagcaaatac gtggagaata tcacttaggt
     8101 gatgttgatt tgtattatcc cggtgccagt cttggcatag tagaagtcga tcctgaaaca
     8161 accgatgcag acagtgccct gcatgctgcc gatattgcga tgtatcagga gaaaaaacac
     8221 aaacagaaaa caccttttgt cgcgcatcca gcgctacatt cctgaggcgt attcacatcc
     8281 ttttgattgg tgataacatg cgaatcggta ttatttttcc ggttgtaatc tttattacag
     8341 cggtcgtatt tttagcatgg ttttttattg gcggctatgc tgccccggga gcataaagat
     8401 gaaaaaaaca acgattatta tgatgggtgt ggcgattatt gtcgttctcg gcactgagct
     8461 gggatggtgg taacgtcacc tctaaaaaat agcaaaggct gcctgtgtgc agcctttgtg
     8521 caatttaagc gttaactttt aatcttcctg tagataaata gcacgacaat cgcaccaata
     8581 acggcaacca cgaagctgcc aaaattgaag ccatcgactt taccaaagcc aaacagtgtg
     8641 ctgatccatc cgccgactac ggcaccgact atccccagca gaatagtcat aaagaatcca
     8701 ccaccatctt tacctggcat gatccacttc gccagaatac cggcaataag cccaaaaata
     8761 atccatgaca gaatgcccat tgtttcctca cttatctgtt ttgcattagc gggttagtcg
     8821 ctgataaaaa gcatagcaca acatcgggag ggcaagattt gtgacgagca tcacggagga
     8881 tttttttgcg atggcgcaga aattgcgcca tcaacgatcg gtgataatta ccaaccacaa
     8941 acatcatgtt cgttttccgt gtcttgacat cctccacgcc ctgaatgacg aggatccctg
     9001 ctacgttcag gctgtcgcct gaatcatttc ggtgggttcc tgcttcaacg agcggcctga
     9061 ctgcaccatc tctccacagg caagaacggc gtgccccgcc gctaaaatgt tacgagcgcc
     9121 gtttacatcg gcgttcgctg tatatccaca tacctggcat ctgaattgac tttgtgacag
     9181 gcgattttct ttcgctgtat gaccacagca cgcgcaacgc tggcttgtgt acgctggcgg
     9241 aacagcaagc acctgaccgc cac
//
LOCUS       DAPHDW010000162         9237 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.165, whole genome shotgun sequence.
ACCESSION   DAPHDW010000162 DAPHDW010000000
VERSION     DAPHDW010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 9237)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9237)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9237
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.165"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..181)
                     /locus_tag="RH057_003897"
     CDS             complement(<1..181)
                     /locus_tag="RH057_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HDV3130197.1"
                     /translation="MASVSISCPSCSATDGVVRNGKSTAGHQRYLCSHCRKTWQLQFT
                     YTASQPGTHQKIIDMA"
     gene            214..882
                     /locus_tag="RH057_003898"
     CDS             214..882
                     /locus_tag="RH057_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV3130198.1"
                     /translation="MSKLAASPKHRTWITEALRLHFEEHLPRVVAGRRLGVPKSTVCS
                     MFVRFRRAGLSWPLPAGMSEQELDACLYGQFSTVPVVRPESTVISEAPVVKKRPRRPN
                     FPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYRKGGLLPSGKNMPALLP
                     VTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLKGKLTPALLQTLIREIK
                     GSSH"
     gene            882..1229
                     /gene="tnpB"
                     /locus_tag="RH057_003899"
     CDS             882..1229
                     /gene="tnpB"
                     /locus_tag="RH057_003899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3130199.1"
                     /translation="MISLPAGSRIWLVAGITDMRNGFNGLASKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTKRLERGRFVWPVTRDGKVHLTPAQLSMLLEGINW
                     KHPKRTERAGIRI"
     gene            1249..2820
                     /locus_tag="RH057_003900"
     CDS             1249..2820
                     /locus_tag="RH057_003900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV3130200.1"
                     /translation="MDTSLAHENARLRALLQTQQDTIRQMAKYNRLLSQRVAAYASEI
                     NRLKALVAKLQRMQFGKSSEKLRAKTERQIQEAQERISALQEEMAETLGEQYDPVLPS
                     ALRQSSARKPLPASLPHETRVIRPEEECCPACGGELSSLGCDVSEQLELISSAFKVIE
                     TQRPKLACCRCDHIVQAPVPSKPIARSYAGAGLLAHVVTGKYADHLPLYRQSEIYRRQ
                     GVELSRATLGRWTGAVAELLEPLYDVLRQYVLMPGKVHADDIPVPVQEPGSGKTRTAR
                     LWVYVRDDRNAGSQMPPEVWFAYSPDRKGIHPQNHLAGYSGVLQADAYGGYRALYESG
                     RITEAACMAHARRKIHDVHARAPTDITTEALQRIGELYAIEAEVRGCSAEQRLAARKA
                     RAAPLMQSLYDWIQTQMKTLSRHSDTAKAFAYLLKQWGALNVYCSNGWVEIDNNIAEN
                     ALRGVAVGRKNWMFAGSDSGGEHAAVLYSLIGTCRLNNVEPEKWLRYVIEHIQDWPAN
                     RVRDLLPWKVDLSSQ"
     gene            complement(2852..3291)
                     /locus_tag="RH057_003901"
                     /pseudo
     CDS             complement(2852..3291)
                     /locus_tag="RH057_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            3899..7312
                     /locus_tag="RH057_003902"
     CDS             3899..7312
                     /locus_tag="RH057_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDV3130201.1"
                     /translation="MEQSLNFEMLRSQWPELAELACMAERYVHSDPESCLVKLRNYTE
                     LMVRWLYRQERLPEGIKANLYDLMNADVFTSMMPEAIIMKMDALRIHGNRAAHGGRIK
                     AKDTYWLLKEAYLLGIWLYVRYAHGNVDDCPKFTLPPLTQSSGRADEKRLEDAIRAQD
                     ESRERELALQRALQQEQEKAEHLTQRLNEARARNQHVADILSIDEAETRRRLIDSRLL
                     AADWNVGEGLKNTDQVTQEHPVKEQPTATGDGYADYVLWDEAHKPLAVVEAKKTSVNA
                     EQGRIQARLYADWLEKEYDQRPIIFYTNGYDIWLWDDHKTHGYPPRRVFGFYSKESLQ
                     YLIQQRETRLPLNSVPHVKDNEGKAVAGRLYQLETIARVSERFTNKYRQSLIVQATGT
                     GKTRVAIALSKLMIDARWVKRVLFLCDRKELRKQAANAFNQFTNEPLYVVGKSKKADR
                     QNARIYIATYPGMMKIMDHFDVGYFDLIIADESHRSIYNVYGDLFKYFDALQIGLTAT
                     PIDMVSKTTFGLFGCEGRIPTANYSLEDAIADNNLVPYEVVTHTTGFLREGIKREKLS
                     DAQIRELEEQGIDPNTLEFDGKALDEAIYNKDTNRYILRNLMENGLKDRDGQLPGKTI
                     IFARNHKHALLLNELFDDMYPQFAGRFCQVIDNYDPRAEQLIDDFKGLDESTNKELTI
                     AISVDMLDTGIDVPEIVNLVFAKPVKSKVKFWQMIGRGTRLCPGLYGYDDNGKPLDKQ
                     KFRIFDHWGNFEYHELHTEEAEVTATKSLAQKRFEAWVMLGAAAQRKFDKQAVDLVAH
                     QLREQINALDEKSIAVQEKWHQKAQYSDEKVLRQLSPKTQQDLLSVLAPLMQWLDVRG
                     QSDAMRFDMDILAAQTARYTNPEELDVLWPVIVEKVERLPPHLAQVQQQGPRINQLRD
                     LGWWKQASLEELEDIRIHLRGIMHLMEKDATPKFGSIQVDITEDANLIQTENRKTNIR
                     SIDFKLYRQQVQGALEPLFQQNPVLKKIRNGEPVTQSELDELAKLVLIQNPNVDIRAL
                     KEFYPQATASLDKLLRTIIGMDSDAVEVRFAQFAADNSLTSQQLRFLSLLKNHIRDYG
                     TIEMRQLFEQPFTHIHNEGVTGVFPDIEQIARLQKIVEELGVVTDAATV"
     gene            7376..9010
                     /locus_tag="RH057_003903"
     CDS             7376..9010
                     /locus_tag="RH057_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDV3130202.1"
                     /translation="MITGDLKSKIDGLWEDFWVGGITNPLTVIEQITYLMYSRMLDTQ
                     EQRDEKRKQIAGIDFKPRFAPEQQEFRFSHYSNLGSDEMMEVVRDGVFQHFRQLGQAD
                     ASKVTLLGNFMKDARLEIVKPSLLTKAVEVIKNLPLDRGDTKGDLYEYLLSKLTTAGI
                     NGQFRTPRHIIRTMVEMMEPNPARGETICDPACGTGGFLATSYEYLLEKYSSLESIHT
                     EIGTNERGELEEQKIFTGDLLTPWRNHVDNNMFHGYDFDTTMLRIAAMNLIMHGVEAP
                     DIHYQDTMSQSFSTNFPQASKNAFNLILANPPFTGSLDEEDIDSTLSAMVKTKKTELL
                     FLARILQMLKVGGRSATIVPQGVLFGSSKAHQSLRKTLVEDNQLEAVINLPSGVFKPY
                     AGVATAILIFTKGGQTDEVWFYDLQNDGYSLDDKRNPIKDNDLPHLLASWKHYRTLRG
                     LPVDNFMGEKLASLLKQQYPEGIDAGVDFKDRTQAAFIVPKEDIAAQKYDLSINRYKE
                     VVYQAEEYEDPKVILKRLKDLEKEILADLDELEGML"
     gene            9007..>9237
                     /locus_tag="RH057_003904"
     CDS             9007..>9237
                     /locus_tag="RH057_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HDV3130203.1"
                     /translation="MKKNQLVTLGKHIDILSGCAFPSSGFNRNNGVPLIRIRDILSGK
                     TETYYEGSYDLKYLIKKGDLLVGMDGDFNREYW"
BASE COUNT         2425 a         2333 c         2436 g         2043 t
ORIGIN      
        1 tggccatatc aatgattttc tggtgcgtac cgggttgaga agcggtgtaa gtgaactgca
       61 gttgccatgt tttacggcag tgagagcaga gatagcgctg atgtccggcg gtgcttttgc
      121 cgttacgcac caccccgtca gtagctgaac aggagggaca gctgatagaa acagaagcca
      181 ctggagcacc tcaaaaacac catcatacac taaatcagta agttggcagc atcaccgaaa
      241 caccggacct ggatcactga agctttacgt cttcactttg aagaacattt accccgggtt
      301 gtggccgggc gtcgcctggg tgtaccaaaa tcaacagttt gtagtatgtt cgtgcgcttt
      361 cggagagctg gcctttcgtg gcctttgccc gcaggcatgt cggagcagga acttgatgcc
      421 tgcctttacg gacaattttc cacggtacca gtcgtacgtc ctgaaagcac cgttatatcc
      481 gaagcccccg tggtaaaaaa acgtccccgg cggcccaact tcccttatga gtttaaaatc
      541 gccttagtgg agcagtcact gcagcccgga gcctgtgtgg cgcagatcgc ccgggaaaac
      601 ggaatcaacg ataacctgct cttcaactgg cgccatcaat accggaaagg tggcctgctg
      661 ccttccggaa aaaatatgcc ggcactgctt cccgtgacgt taacgccgga gccggataat
      721 aaaatcccgg cccccgcaca ggaaccagag cagataaata caccgtccga cagtctgtgt
      781 tgtgagctgg ttctgccggc cggaactctc aggcttaaag gtaaactgac gccggcgtta
      841 ttacagacac ttatccgcga aataaaaggg agcagccact gatgatatct ctccctgcag
      901 gttcgcgtat ctggctggtt gcaggtatca ccgatatgcg aaatggcttt aacggcctgg
      961 catcaaaagt tcagaacgtc ctgaaggatg acccgttctc cggacacctg ttcatcttcc
     1021 gcggacgccg gggtgaccag ataaaagtgt tgtgggctga cagtgacgga ctgtgcctct
     1081 tcaccaaacg cctggagcgg ggccgcttcg tctggccagt cacccgtgac ggcaaggtgc
     1141 accttactcc ggctcagtta tccatgcttc ttgaaggtat caactggaag cacccgaaac
     1201 gaacggaacg cgctggaatc cgcatataac ccgttgtaaa gtgaggatat ggacacctca
     1261 cttgctcatg agaacgcccg cctgcgggca ctgttgcaga cgcaacagga caccatccgc
     1321 cagatggcga aatacaaccg cctgctctca cagcgggtgg cggcttatgc ttccgaaatc
     1381 aaccggctga aggcgctggt tgcgaaactg cagcgcatgc agttcggtaa aagctcagaa
     1441 aaacttcgcg ctaaaaccga acggcagata caggaagcac aggagcgaat cagcgcactt
     1501 caggaagaaa tggcggaaac gctgggtgag caatatgacc cggtactgcc atccgccctg
     1561 cgccagtctt cagcccgtaa accgttaccg gcctcacttc cccatgaaac ccgggtcatc
     1621 cggccggaag aggaatgctg tcctgcctgt ggtggcgaac tcagttctct gggatgtgat
     1681 gtgtcagagc aactggagct tatcagcagc gcctttaagg ttatcgaaac acaacgtccg
     1741 aaactggcct gttgccggtg cgaccatatc gtgcaggcac cagtaccttc aaaacccatt
     1801 gcacgcagtt atgccggagc agggcttctg gcccatgttg tcaccgggaa atatgcagac
     1861 catctgccgt tataccgcca gtcagaaata taccgtcgtc agggcgtgga gctgagccgt
     1921 gccacactgg ggcgctggac aggtgctgtt gctgaactgc tggagccgtt gtatgacgtt
     1981 ctgcgccagt atgtgctgat gcccggtaaa gtccatgctg atgatatccc cgtcccggtc
     2041 caggagccgg gcagcggtaa aacccggaca gcccggctgt gggtctacgt ccgtgatgac
     2101 cgtaacgccg gttcacagat gcccccggag gtctggttcg cgtacagccc ggaccggaaa
     2161 ggcatccatc cacaaaatca cctggccggt tacagcggag tgcttcaggc cgatgcttac
     2221 ggtggctacc gggcgttata cgaatccggc agaataacgg aagccgcgtg tatggctcat
     2281 gcccggagaa aaatccacga tgtgcatgca agagcgccca ccgacatcac cacggaagcc
     2341 ctgcagcgta tcggtgaact gtatgccatc gaggcagagg tccggggctg ttcagcagaa
     2401 cagcgtctgg cagcaagaaa agccagagcg gcaccactga tgcagtcact gtatgactgg
     2461 atacagactc agatgaaaac actgtcgcgt cactcggata cggcaaaagc gttcgcatac
     2521 ctgctgaaac agtggggggc cctgaacgtg tactgcagta atggctgggt ggaaatcgac
     2581 aacaacatcg cagagaacgc cttacgggga gtggccgtag gccggaaaaa ctggatgttc
     2641 gcgggttccg acagcggtgg tgaacatgcg gcggtgttgt actcgctgat cggcacatgc
     2701 cgtctgaaca atgtggagcc agaaaagtgg ctgcgctacg tcattgaaca tatccaggac
     2761 tggccggcaa accgggtacg cgatctgttg ccctggaaag ttgatctgag ctctcagtaa
     2821 atatcaatac ggttctgacg agccgcttac tcttcaggcg atttacatga ccttcgacaa
     2881 caccgttact ccagcggctg ctggttgcct cacatatagc cgcagcatca gcttccatcc
     2941 ccgcagccac gcgccggagt tctgctgaac cgttttcgtg gactcgagtg aaccaactgc
     3001 ttagatttga tttatttggg gttttcagaa tacggtagaa ctcaagtact agttgctgcg
     3061 ctattttcag cgccggttct ttttcacaca tcagactaat aaatcgggaa gcataatttt
     3121 cttcacccct gattattatc cagggcatca accagcggct tacccgggac actgaaggaa
     3181 gtctggcggc agtagtaact ggtgggttcc agcctttacg ccatttggca acagcatccc
     3241 tgacgatggt ttcactgcct gtaacccccg ggccaccatt tcccgccata tccagtcgct
     3301 ggtgtgctga attggtcatg tatgaaggaa gatatcaatg gtggtatctc cctttcaaaa
     3361 ggaagtgaaa agcttgatgc ctgctgccag gcaatcaata ttacgtgctt ttaacgacta
     3421 tcctaaatgg tggtagctat gcagagctcc cggtcctagc gctgggagct tatcgcttat
     3481 ctttaccgta agcgtaaacg gaccgccgta tgtagccatt aaaccagtat tggtaacgta
     3541 gatacccacc ttttcaaaag ggaagtggat aaaagatagc gaaaatgcga agagttgatt
     3601 aatgcattga aacaaaattc attaatgatg aacttggaga aaagctccat cgttttattg
     3661 gctcaaaaaa catatcccat agatgatcag ccataccttc attggttagc aaggtatcct
     3721 gtgagtagcc atggctgaag atgcccattg atgggcgaaa accaaggatc tgaccgttag
     3781 agcagttatg tcgttaaaca catcaccacc atatgccatc cggctcactt cgtcggccaa
     3841 attagtgtga tattctttcc agaaatgctg ttgtatccac gacataaggc gatgattgat
     3901 ggagcagtct ctcaattttg aaatgttgcg cagccagtgg ccggaactgg ctgaactcgc
     3961 gtgtatggcg gaacgctatg ttcactccga tccggaaagt tgtctggtca agctgcgcaa
     4021 ctacaccgaa ttgatggtgc gctggttgta tcgtcaggag cggttgccgg aaggtattaa
     4081 ggctaatctt tacgatttaa tgaatgctga tgtctttacc agcatgatgc cggaagccat
     4141 catcatgaaa atggatgcgc tgcgtatcca tggcaaccgt gccgcgcacg gcggacgtat
     4201 caaagctaaa gatacttact ggctgctcaa agaagcgtat ttgttgggaa tttggctgta
     4261 tgttcgctac gcccacggta atgttgatga ctgccccaaa tttacactcc ctccattaac
     4321 acaatcttca ggtcgtgcag atgaaaaacg tctggaagat gcaatcaggg ctcaggatga
     4381 aagccgtgag cgcgaactgg cgctgcaacg cgcactacag caagaacagg aaaaggccga
     4441 acacctcact caacgcctga atgaagccag agcgcgtaac cagcatgttg ccgatattct
     4501 ctctattgat gaagcggaaa cccgccgtcg cctgattgac tctcgcctgc ttgctgctga
     4561 ctggaatgta ggcgaaggcc ttaaaaatac cgatcaggtt acgcaggaac atcccgttaa
     4621 agaacaacct accgcgaccg gagacggtta tgcggactat gtcttgtggg atgaggcaca
     4681 caaaccgctg gcggtggtgg aagcaaaaaa aaccagcgtc aatgccgagc aaggacgaat
     4741 tcaggcccgg ctgtatgcag actggctgga aaaagaatac gaccagcgtc cgatcatctt
     4801 ctacaccaac ggctatgata tctggctgtg ggatgaccat aaaactcatg gttatccccc
     4861 gcgtcgggtg tttggcttct acagcaagga aagcctgcaa tatcttattc aacagcgtga
     4921 aacccgtctt ccgctgaaca gtgtgccgca cgtaaaagat aacgaaggta aggccgttgc
     4981 cggacgtttg taccagcttg aaaccattgc ccgtgtcagc gagcggttta ccaacaaata
     5041 ccggcaatcg ctaattgttc aggctacggg tacaggcaaa acccgcgtgg caatagcgct
     5101 aagcaaacta atgattgatg cccgctgggt aaaacgcgtc ctgtttcttt gcgaccgcaa
     5161 ggaactacgt aaacaggcgg cgaatgcctt taatcaattc accaatgagc cgctgtatgt
     5221 ggtcgggaaa tcgaaaaaag cagacaggca gaatgctagg atctacatcg ccacctatcc
     5281 tggtatgatg aaaatcatgg accatttcga tgttggttat ttcgatttga tcatcgccga
     5341 tgaatcccat cgctctatct acaacgttta cggcgatctg tttaagtatt ttgatgccct
     5401 ccagattggc ctgacggcca cgccgattga catggtgagt aaaaccactt tcggcctgtt
     5461 tggttgtgaa ggacgtattc ctaccgccaa ctacagcctg gaagatgcca tcgccgataa
     5521 caatctggtt ccctatgagg ttgtcacgca caccacagga ttcctgcgcg aaggcatcaa
     5581 acgggagaaa ttaagcgacg cacaaatccg cgagctggaa gaacaaggca tagatcccaa
     5641 tacgctggag tttgatggca aggcgctgga tgaggcgatc tacaacaaag acaccaaccg
     5701 ctatatcctg cgtaacctga tggaaaatgg cctgaaagat cgggacggcc agttgccagg
     5761 taaaaccatc attttcgccc gtaaccacaa acacgcgctg ctgttgaatg aattattcga
     5821 cgatatgtac ccgcagtttg ccgggcgctt ctgccaggtc atcgacaatt acgatccccg
     5881 cgccgagcaa ctgattgatg attttaaagg gctggatgaa agcaccaaca aagagctgac
     5941 catcgctatc tccgttgaca tgctcgatac cggtattgat gtcccggaaa ttgtgaatct
     6001 ggtctttgcc aaaccggtca aatcaaaagt gaagttctgg cagatgattg gccgtggtac
     6061 gcgcctctgt ccggggttgt acggttacga cgataacggc aaacctctgg ataaacagaa
     6121 attccgcatt ttcgatcact ggggcaattt tgagtatcac gaactgcata ccgaagaggc
     6181 cgaagtcaca gcgacaaaat cgcttgcaca aaaacgcttc gaagcgtggg ttatgctggg
     6241 ggccgctgcg caacgtaaat tcgacaaaca ggcggtggat ttagttgctc accagctacg
     6301 cgagcaaatc aatgcactgg atgaaaagtc gattgccgtc caggaaaagt ggcatcaaaa
     6361 agcgcagtac agcgatgaaa aagtgctgcg ccagctttcc ccgaaaacac agcaggattt
     6421 gctatctgtc ctggccccgc tgatgcagtg gctggacgtg cgcgggcaaa gcgacgctat
     6481 gcgctttgat atggatattc tggcggcgca aactgcccgt tataccaacc cggaagagct
     6541 ggatgtgctc tggccggtca tcgtcgaaaa agtggagcgc ctgccgccgc atttggcgca
     6601 ggtccaacag caaggaccgc ggatcaatca gcttcgtgat ttaggctggt ggaagcaggc
     6661 cagcctggaa gagctggaag atatccgcat tcatctgcgc ggcatcatgc acctgatgga
     6721 aaaagacgcg acgccgaaat ttggttcgat acaggtggat attaccgaag atgcgaacct
     6781 gattcagacg gaaaaccgca aaaccaacat ccgctcgatt gacttcaaac tctatcgcca
     6841 gcaggtgcag ggagcgctgg agccgctgtt ccagcaaaat ccggtgctga agaaaatccg
     6901 caacggcgag cctgtgacgc aaagcgagct ggatgaactg gctaaactgg tgctgatcca
     6961 gaaccctaac gttgatatcc gggcactgaa agagttctac ccgcaggcga ccgccagcct
     7021 ggataaacta ttgcgtacca tcatcgggat ggacagcgac gcggtggaag tgcgctttgc
     7081 tcagttcgcc gctgataaca gcctgaccag ccagcaattg cgcttcctgt cattgctgaa
     7141 aaaccacatt cgcgattacg gcaccattga aatgcggcag ctctttgaac agccttttac
     7201 gcatatccac aacgaaggcg ttaccggtgt gttccctgat atagagcaga tcgcccgcct
     7261 acaaaagata gtcgaagagc tgggtgttgt gaccgacgca gcaacggtat aatacgcaca
     7321 tgacaagccc cggcagcggg gcttttgtat ttaaaacagg atataaaagc taacaatgat
     7381 cactggtgac ttaaaaagta aaatcgacgg cctgtgggaa gatttctggg tgggtggtat
     7441 caccaacccg ctgaccgtaa tcgaacagat cacctatcta atgtattccc ggatgctgga
     7501 tactcaggaa caacgcgacg agaagcgtaa acaaatcgcg ggtattgatt ttaaaccgcg
     7561 ttttgcacca gaacagcagg agttccgttt cagtcactat agcaatcttg gctcggatga
     7621 gatgatggaa gtggtgcgcg atggtgtatt ccagcatttt cgtcagctcg gccaggctga
     7681 tgcgtcgaag gtaacgctgc tgggcaactt tatgaaagat gcccgtctgg agattgttaa
     7741 gccttcattg ctgaccaaag cggttgaggt gatcaaaaac ctgccattgg atcgcggcga
     7801 caccaaaggc gacctttacg aatacctgtt aagcaagctg accactgccg gaatcaacgg
     7861 ccagtttcgc acaccgcgcc acattatccg cacgatggtt gaaatgatgg agccgaaccc
     7921 cgcccgcggc gagacgattt gcgatccggc ctgcggcacc ggtggctttc tggcaaccag
     7981 ctatgaatat ctactggaga agtacagctc gctggaatcc attcataccg agattggcac
     8041 caacgaacgt ggtgagctgg aagagcaaaa aatctttacc ggcgatctgt tgaccccgtg
     8101 gcgtaaccat gtggataaca atatgttcca cggttacgac tttgacacca caatgttacg
     8161 tatcgccgcc atgaacctga ttatgcacgg cgtggaagcg ccggatatcc actatcagga
     8221 caccatgagc cagagcttca gcacaaactt cccgcaggcc agtaaaaacg ctttcaacct
     8281 gattctggca aacccgccgt ttaccggttc actggacgaa gaagatatcg actccacgct
     8341 gtcggcaatg gtgaaaacca aaaaaaccga actactgttc ctggcgcgta ttctgcaaat
     8401 gctgaaagtg ggcgggcgca gtgccactat cgtgccgcag ggcgtgctgt ttggctccag
     8461 taaggcgcac cagtcacttc gcaaaacgct ggtggaagat aaccaactgg aagcggtgat
     8521 caacctgcct tccggcgtat ttaaacctta cgctggcgtg gcgacggcga tcctgatctt
     8581 taccaaaggc ggtcagacgg acgaggtctg gttctacgat ctgcaaaatg atggctacag
     8641 cctggatgac aagcgtaacc caattaaaga caacgacctg ccgcatctgc tggcaagctg
     8701 gaagcattac cgtactttac gcgggctgcc ggttgataac tttatgggtg agaagttagc
     8761 ctcgttgctg aaacagcagt acccggaagg gattgatgct ggcgttgatt ttaaagatcg
     8821 cactcaggcg gcgtttatag tgccaaaaga agatattgct gcccagaaat acgatctctc
     8881 tattaatcgt tataaagaag tggtgtatca ggctgaggag tatgaagatc cgaaggtgat
     8941 attgaagcgg ttaaaagatc tggaaaaaga gattctggcg gatctggatg agctggaggg
     9001 gatgctgtga aaaaaaatca attggtcaca cttggcaaac atatagacat attgtccggg
     9061 tgtgcattcc cttcaagtgg attcaatagg aacaatggtg ttccactaat ccgtattaga
     9121 gatattcttt caggaaaaac cgaaacttat tatgaaggca gttatgactt aaaatatctt
     9181 attaaaaaag gtgatttact agttgggatg gatggagatt tcaaccgtga gtattgg
//
LOCUS       DAPHDW010000163         8947 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.166, whole genome shotgun sequence.
ACCESSION   DAPHDW010000163 DAPHDW010000000
VERSION     DAPHDW010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8947)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.166"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            66..338
                     /locus_tag="RH057_003905"
     CDS             66..338
                     /locus_tag="RH057_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HDV3130204.1"
                     /translation="MALPRITQKEMTEREQRELKTLLDRARIAHGRVLTNSETNSIKK
                     EYIDKLMVEREAEAKKARQLKKKQAYKPDPEASFSWSANTSTRGRR"
     gene            complement(339..1211)
                     /gene="rluF"
                     /locus_tag="RH057_003906"
     CDS             complement(339..1211)
                     /gene="rluF"
                     /locus_tag="RH057_003906"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HDV3130205.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDVVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKVKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1423..2112
                     /gene="pepE"
                     /locus_tag="RH057_003907"
     CDS             1423..2112
                     /gene="pepE"
                     /locus_tag="RH057_003907"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HDV3130206.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTAKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(2203..3834)
                     /locus_tag="RH057_003908"
     CDS             complement(2203..3834)
                     /locus_tag="RH057_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HDV3130207.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNATTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(4054..7737)
                     /gene="metH"
                     /locus_tag="RH057_003909"
     CDS             complement(4054..7737)
                     /gene="metH"
                     /locus_tag="RH057_003909"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HDV3130208.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLV
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGEDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKTDDTANAQQAEWRSWEVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRTAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFTIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTRKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDYA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            7937..8761
                     /gene="iclR"
                     /locus_tag="RH057_003910"
     CDS             7937..8761
                     /gene="iclR"
                     /locus_tag="RH057_003910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HDV3130209.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
BASE COUNT         2061 a         2473 c         2307 g         2106 t
ORIGIN      
        1 ttatcagttc agctcgcaaa cgaattcccg tacactgaac tctctggctc actgagggca
       61 ccatcatggc actcccccgt attacccaaa aagagatgac tgaacgcgag cagcgtgaac
      121 ttaaaacact gctggatcgg gcgcggattg cgcatggtcg cgtactgacc aactccgaaa
      181 ccaacagcat taagaaagag tacatcgata aattaatggt cgagcgtgag gctgaagcga
      241 aaaaagcccg ccagttgaag aaaaagcagg cttataaacc ggatcctgaa gcgtcattta
      301 gctggtcagc aaatacgtcg acgcgcggca ggcgctgatt agcgcccctt ctttttacgc
      361 cccggagagg taaaacgctt accgttggac gccgggcgac cgcctttttc cgccgttttt
      421 tccatcttaa cgactggacg tttgatgccc gctgttttcg gtttggcctt cactttaggt
      481 tttgcctcgg aagaggaatt ttcgatgagc ttaaagaggt cgattaactc atcatcagtt
      541 aaatcgcgcc actcacccag cggaatgccg cttaagctga cgttcataat gcgcgtacgt
      601 tccagctttt tcacttcata gccgaaatgc tcgcacatgc gccggatctg acggttcagc
      661 ccctgcacca gggtaatgcg gaagacaaac ggcgcttctt ttttgacttt gcactttttc
      721 gtcaccgtcc cgaggatcgg cacccccgcc cccataccac gaataaattc atcggtaatc
      781 ggtttatcga ccgtcaccag atactctttc tcatgatcgt tacccgcacg gaggatctta
      841 ttcaccagat cgccgtgatt ggtgaggaaa atcagcccct gggagtcttt atccaggcgg
      901 ccaatcggga acacgcgttt gctgtggtta acgaaatcga caatgttatc gcgctcgcca
      961 tcttcggtgg tgctgacaat accgacgggc ttgttcaggg cgataagtac caaatcttcg
     1021 gcttcccgag gctcaatcaa ctgaccgttt actttcacaa cgtcgccggg tttcacctga
     1081 tcgccaatgg tggctcgctt gccattaagg aacacattgc cttgctcgat atagcgatcc
     1141 gcttcgcggc gtgagcaaat tccgctttcg ctgatgtatt tatttaaacg gactgatgag
     1201 tcgggcagca taaattctcc tgtaaaaaac ggaatatacc gtacctttgg gttgataaaa
     1261 aatagtcatg agcgaactat ttgttatttc cattggcttg aattcgttac ctgctcaaat
     1321 cactttccag aagcaatgtg cgacatcgtg agccatgtca gtaagtggtt aaattcgcag
     1381 tggtagcatg cctcccgtga tttcttacta accggagaag taatggaact gcttttattg
     1441 agtaactcga cgctgccggg taaagcctgg ctggaacatg cactgccgct aattgctgaa
     1501 cagttgcagg gtcgccgctc agcggtgttt atccctttcg ctggcgtaac gcagacctgg
     1561 gatgattaca cagcgaaaac ggctgcggtt ctcgctccgc tgggtgtttc tgtcaccggt
     1621 attcatagcg ttgtcgatcc cgttgccgcg attgaaaatg ctgagatcgt gattgtcggc
     1681 ggcgggaata ctttccagtt gctgaaacag tgccgcgagc gcgggctgct ggcaccaatt
     1741 actgacgtgg ttaaacgtgg cgctctgtat attggctgga gcgcaggcgc taaccttgct
     1801 tgcccaacca ttcgtaccac caacgatatg ccgattgtcg atccgcaagg tttcgatgcg
     1861 ctaaatctgt tcccgttgca aatcaacccg cacttcacca acgcgctgcc ggaaggccat
     1921 aaaggtgaaa cccgtgagca gcgtattcgc gaactgctgg tcgtcgcgcc agaactgacg
     1981 attattggtc taccggaagg taactggatc acagtgagta aaggtcacgc tacgctgggt
     2041 ggcccgaaca ccacttatgt gtttaaggct ggtgaagaag cggtgccgct agaagctggt
     2101 caccgttttt aagtgaattg ccggatgcat gatccggcat cttgttcata gtgcctgatg
     2161 cgacgcttga cgcgtcttat caggcctaca ggattcacaa tattaatact cgtcccgtcc
     2221 ttcatcttca tccggctgtt ccagcacact gtaagccacc gaacaaaaca gcgagttcag
     2281 gcgctgcata tctcccagta agcctaaatg tagcgaactg gtttcaatgc tctgcacgtt
     2341 ttgctgatgc aggcgatcga cgtgggcgtg ggaatagcgg cgattaagaa tgcgaaagcg
     2401 atgcttacta cggcgcaaac gacgagcgct ggtgacatcg ccagagaaga acaccgacat
     2461 tgccagtttt aaattactga gcaattgctc atagagcgca tccagttcct tcaagccatc
     2521 aagcgaaaat gcccgccgtg ctgccagcga tttatcggca atttcgctgc ccatgcgctc
     2581 gacgatatcg gaagcctgtt caaggttgag cgacatttca ataatctccg cccagcgacg
     2641 cgactcttct tctgccagtt cctcttttgg catccgcgcc agatacagct taatggcggt
     2701 atagagaacg ttgatatcat ctgccagctt acgcagctct ttctcctgcc gtggctcgcc
     2761 gtgcatcact ttattcagcc cttccatcat ctgttccatg gcgtcgccaa tgcgcagggt
     2821 ttcgcgcgcg gcgttcgcca gagcaagcgt gggcgtatcc agcgcgctga catccagatg
     2881 tttaggccgt agctgggtat ccagttccgg ttcatcgcga ataatcgttt tgcaaaaccg
     2941 tgccatcggg tcaacaaatg gcagcatgac caggcaacgt acaaggttgt agaagacgtg
     3001 gaaatagatc accagttccg cttttggcaa tgacaacttc cccattgtct ctgccagcaa
     3061 atggacaaac ggcaggataa tcaggctacc caccagctta aacagcaaac tacccagcgc
     3121 gacacggcgg gctgcggcat tggcggcact gttgttgagc atcgccagca ggccggaacc
     3181 gaggttagca ccaatcacca gacagagcgc cacggggaag gagataatgc ctgcggcggt
     3241 cagagtcgca gtcagcagta cagcagcaag gctggagtag ctgataatgg cgaacatcgc
     3301 gccaatcagc gcatccagca gaatatcgcc ggtcagcgag gcaaagatca cctgaacgcc
     3361 gtttgcctgg gtgatcggcg ttacggcctg cacaatcaac tccagcgcca gcaaaatcag
     3421 cccaagacca ataccgacgc ggcccagttg cccggcgcgt gactgtttgc gtccgaggaa
     3481 aaaaatcacg ccgataaaaa taagtaacgg tgacagccag gataagtcga aggtgagaat
     3541 acgcgccatt agcgccgtcc cgacatctgc acccagcaca atgaccagag ccggtgcgag
     3601 ggctaccaga tcctgagcga caaacgaggt caccagcatg gtggtggcat tactgctctg
     3661 caccagtgcg gtaacgccga tccccgcgca aaaggcgagc ggcttttttt cgacgctccg
     3721 gctaaggaca gtacgcaaac gcgcgccgaa gacgcgcatt acgccggttc gaacaatatg
     3781 agtcccccag accagcaggg cgacggcaga aagcaggtga agcagcgtta acacgagttc
     3841 aggttctcct tatcgttata tgttcctgaa gcaattgaat gaccaaagga ccggaaacac
     3901 gctgactggc atctttactg tgccttttga cattcgtttt tccggctaag attattccag
     3961 cctgtttcca gtataagtgt ttcatattaa taaagagaca gggcactcat tgagcgccct
     4021 gtttgttgta aggaaaagtg acagatttgt gaatcagtcc gcgtcatacc ccagattcgg
     4081 tgccagccag cgctcaacat cggagacgct cataccttta cggcgggcat agtcttcaac
     4141 ctgatcgcgc tgaatttgcg ccaccgcgta gtacttgctg tccgggtggc tgaaatacca
     4201 acccgaaacc gatgcaccgg gccacatggc gaaagattct gtgagtttca tgccggtatg
     4261 tttttccact tccagcagct cccagatggt ggctttttcc gtatgttccg ggcaggccgg
     4321 gtagcccggt gccggacgga tgccctggta gttttcgcgg atcagttctt cgttgctgag
     4381 gttctcgttc ggcgcatagc cccagtagac tttacgcaca cgctcatgga gatactccgc
     4441 aaaggcttcg gctaaacggt cggcaagcgc tttcaccatg attttgttgt aatcatcgtg
     4501 ctgcgcttca aaggcatcag ccagtgcgtc ctcttccagc ccgccagtca cggcaaatgc
     4561 gccgatgtaa tctgctttac cagaaagctt cggcgcaacg aagtcagcga gacagtagtt
     4621 agcgaatccc gttttttcgg tctgttgacg cagatggtgg ctgacgttga tcacatgggt
     4681 gcgcgtttcg tcacggtaga tttcaatgtc atcgcccaca cggtttgccg ggaacaggcc
     4741 caccacgcca cgcggattca gcgttttctc ggcgcttaat ttatccagca tgtcgttggc
     4801 gtctttaaac agccgctgcg cctcaacgcc caccacttca tcttccagaa tgcgcggata
     4861 cttcccggcc agcgaccagg tcataaagaa cggtgtccag tcgatgtaat tacgcagcgt
     4921 ttcgatgctg gcttcaactt cctgcacgcc gagacggtgc gccaccggcg gcgtgtaagc
     4981 ctgccagtca aaagcgaaat cgttatcgcg cgccgcttcc agcgtgaccg gtggtgtgcg
     5041 cggtttctta cgcccgtgct gaatacgtac ggtttcgtac tccttgcggg tacgagcgac
     5101 aaaatcatca cgctgagtat cggaaagcag cgccgccacc acaccaacgg tgcgcgatgc
     5161 gttctgtaca tacaccgtcg ggccgctgta gttctgctca attttcaccg ccgtgtgcgc
     5221 ttttgaggtc gtcgcgccgc caatcaatag cgggatggtg aagccctgac gctccatctc
     5281 tttcgccacg ttaaccatct cgtccagcga cggcgtgata agccccgaaa gtccaatcag
     5341 atcagcattc acttctttag cggtacggag aattttttcc gcaggcacca taacgccgag
     5401 atcgacaatt tcgtagttgt tacattgcag caccacacca acgatatttt taccgatgtc
     5461 gtggacgtcg cccttcacgg tggcgatcac catcttgccg ttggttttgc cctgctcttt
     5521 gctggcttca ataaacggtt cgagatacgc caccgcctgt ttcatgacgc gcgccgattt
     5581 gaccacctgt ggcaggaaca ttttcccttc gccaaacaga tcgccgacca cgttcatgcc
     5641 gtccatcaac gggccttcaa tcacttcaat cgggcgcgta gcctgctggc gggcttcttc
     5701 ggtatcctgc tcgataaact cggtaatgcc tttaaccagc gagtattcca gacgtttatt
     5761 cacttcccac gagcgccact ccgcctgctg ggcgttagcg gtgtcgtcgg ttttgctgcc
     5821 gcgatatttc tcggcaagct ccagtaaacg ctcagtgcca tcgtcgcgac ggttaagaat
     5881 cacatcttcc accgcgtcgc gcagttcagc gggcaggtcg tcgtaaatcg ccagttgccc
     5941 ggcgttgacg atccccatat ccatgccatt gcgaatagcg tagtagagga acaccgcgtg
     6001 aatggcttcg cgcaccggat cgttgccacg gaatgagaaa gaaacgttag atacaccgcc
     6061 ggaaatcagc gcgtgcggca gttcgcgttt gatatcttca cacgcgccga taaagtcctg
     6121 cgcgtagttg ttgtgctctt caatgccggt tgcgaccgcg aagatgttcg ggtcgaagat
     6181 gatatcttct ggcgggaagc caacctcttc ggtgaggatt ttgtacgccc gacggcaaat
     6241 ctcgattttc cgtgcgcggg tatcggcctg tccctgttcg tcaaaggcca ttaccaccac
     6301 tgccgcaccg taacgacgca agagcttcgc gtgatggata aaggcgtcga cgccctcttt
     6361 catcgagata gagttaacaa tgcctttgcc ctggatacac ttcagacctt tttcaatgac
     6421 atcccatttt gaggagtcga tcataatcgg cacgcgagcg atatccggtt caccggcaat
     6481 cagattgaga aaacgcacca tcgccgcttc ggcgtcgagc atcccttcat ccatgttgat
     6541 atcgataatc tgcgcaccgt tttccacctg ctggcgggcc acatccagtg cctcgctgta
     6601 tttctcttct ttgatcaggc gcttgaactt agcggaaccg gtgacgttgg tgcgttcacc
     6661 cacgttcaca aacaggctat cttcgccaat gttcagcggc tccaggccgg acaaacggca
     6721 ggctacgggg atttccggca gtttgcgcgg cgctaatcct tctactgcac gactcatcgc
     6781 tgcaatatgt tgtggcgtgg tgccacagca gccgccgacg atattgagaa aacccgcttg
     6841 cgcccattca cgtatctgtt ttgccatcgt gtcggcgtcg agatcgtact caccaaaggc
     6901 gttgggtagc ccggcgttcg ggtgcgcggt gacgtagcat tccgcaatcc gtgacagctc
     6961 ctgcacgtac tggcgcagtt catcgggccc cagcgcacag ttcaggccaa aggtcagagc
     7021 ttcggcgtgg cgcaatgagt tgtaaaatgc ttcggtggtc tgcccggaga gcgtgcgccc
     7081 ggaggcgtcg gtgatggtgc cggagatcat aatcggcagc tcaacgccca gcgcttcaaa
     7141 ctccgttttc accgcaaaaa ccgccgcttt ggcgttaagg gtgtcgaaaa cggtttcaat
     7201 caggatcaga tccgcaccac cttccaccag cgctttggtg gattcgcgat aggccgccac
     7261 cagctggtca aaagtgatgt tgcggaatgc cggatcgttg acgtccggag aaatagacgc
     7321 cgtgcggttg gttgggccga gaacaccggc aacgtagcgc ggtttctccg gcgtgcgcgc
     7381 ggtccactcg tcagcacagg cgcgcgccag tttcgccgcc gcaaagttga tttccgccga
     7441 cagggattcc atctggtaat ccgccatcgc aatggtcgtg gagttgaagg tgttggtttc
     7501 gatgatatcc gcgcccgctt caaagtaggc gttgtggata gcggcgatca cttccggttt
     7561 gctgagcacc agcaggtcgt tgttgccttt gagatcgcat ggccagtcgg caaagcgttc
     7621 accacgaaaa tcggcttcgt tcagtcgata gctctggatc atggtgccca taccgccgtc
     7681 cagcaccaga atacgttcat ttaactgcgc acgcagttgt tccactttgc tgctcacact
     7741 tgctcccgac acgctcaacc cagatttaat aaaaattcaa caaaccatac tggcataaac
     7801 gcatctgtgg taaaagcgac caccacgcaa catgagattt gttcaacatt aactcatcgg
     7861 atcagttcag caactattgc attagctaac aataaaaatg aaaatgattt ccacgataca
     7921 gaaaaaggag gctgtcatgg tcgcacccat tcccgcgaaa cgcggcagaa aacccgccgt
     7981 tgccaccgca ccagcgactg gacaggttca gtctttaacg cgtggcctga aattactgga
     8041 gtggattgcc gaatccaatg gcagtgtggc actcacagag ctggcgcaac aagccgggtt
     8101 acccaattcc acgacccacc gcctgctaac cacgatgcaa cagcagggtt tcgtgcgtca
     8161 ggtcggcgaa ctgggacatt gggcaatcgg cgcacatgcc tttatggtcg gcagcagctt
     8221 tctccagagc cgtaatttgt tagcgattgt tcaccctatc ctgcgcaatt taatggaaga
     8281 gtctggcgaa acggtcaata tggcggtgct tgatcaaagc gatcacgaag cgattattat
     8341 cgaccaggta cagtgtacgc atctgatgcg aatgtccgcg cctatcggcg gtaaattgcc
     8401 gatgcacgct tccggtgcgg gtaaagcctt tttagcccaa ctgagcgaag aacaggtgac
     8461 gaagctgctg caccgcaaag ggttacatgc ctatacccac gcaacgctgg tgtctcctgt
     8521 gcatttaaaa gaagatctcg cccaaacgcg caaacggggt tattcatttg acgatgagga
     8581 acatgcactg gggctacgtt gccttgcagc gtgtattttc gatgagcacc gcgaaccgtt
     8641 tgccgcaatt tcaatttccg gaccgatttc acgtattacc gatgaccgcg tgaccgagtt
     8701 tggcgcgatg gtgattaaag cggcgaagga agtgacgctg gcgtacggtg gaatgcgctg
     8761 actttttctg gcgggcagag gcaatattct gcccatcata cctgagtggc aatagaataa
     8821 gggtgtctgt taatcgcatt gacgccaaaa taacttaatg tcatacactt cacacacttt
     8881 tattaactca gcattatttt taaacatcaa actacttaat cattaacaaa atcgcaagat
     8941 ctcattc
//
LOCUS       DAPHDW010000164         8940 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.167, whole genome shotgun sequence.
ACCESSION   DAPHDW010000164 DAPHDW010000000
VERSION     DAPHDW010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8940
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.167"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(166..1266)
                     /gene="chaA"
                     /locus_tag="RH057_003911"
     CDS             complement(166..1266)
                     /gene="chaA"
                     /locus_tag="RH057_003911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HDV3130210.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLALVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQTKTHQSLFVYEHEDDSDDDDPHHGKPSAH
                     SSVWHAIWLIIHLIAVIAVTKMNASPLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            1536..1766
                     /gene="chaB"
                     /locus_tag="RH057_003912"
     CDS             1536..1766
                     /gene="chaB"
                     /locus_tag="RH057_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HDV3130211.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKDDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            1924..2619
                     /gene="chaC"
                     /locus_tag="RH057_003913"
     CDS             1924..2619
                     /gene="chaC"
                     /locus_tag="RH057_003913"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HDV3130212.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     EPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENFPDGVLRPGFA"
     gene            complement(2663..3016)
                     /gene="ychN"
                     /locus_tag="RH057_003914"
     CDS             complement(2663..3016)
                     /gene="ychN"
                     /locus_tag="RH057_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HDV3130213.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            3201..4595
                     /gene="ychO"
                     /locus_tag="RH057_003915"
     CDS             3201..4595
                     /gene="ychO"
                     /locus_tag="RH057_003915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HDV3130214.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDDGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLLDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSVEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGERASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            complement(4596..5246)
                     /gene="narL"
                     /locus_tag="RH057_003916"
     CDS             complement(4596..5246)
                     /gene="narL"
                     /locus_tag="RH057_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HDV3130215.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(5239..7035)
                     /gene="narX"
                     /locus_tag="RH057_003917"
     CDS             complement(5239..7035)
                     /gene="narX"
                     /locus_tag="RH057_003917"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HDV3130216.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            7374..8765
                     /gene="narK"
                     /locus_tag="RH057_003918"
     CDS             7374..8765
                     /gene="narK"
                     /locus_tag="RH057_003918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HDV3130217.1"
                     /translation="MSHSSAPERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATDTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
BASE COUNT         2181 a         2174 c         2308 g         2277 t
ORIGIN      
        1 aagagtacat tccggctttt caacagctgt tgcagtggtt tcgtgcaggt tgtggtacag
       61 gctcgcaatt ctgattaacg agctgtccag gtagtatgaa gaaatacaat aagcaggaat
      121 ttatcttatc tcccccgatt tccgtcatcg gaggagatat aactgtcagg caaatatcgt
      181 catcaaatag gcggcaaaca gtgccagatg cgctgcgcca ttgagcacgt tagtacgtcc
      241 ggtggagaag gagatatggc acagcactaa agaggccacc atcaccacca tttctggcgc
      301 accaagtgca aactgtaatt cgttacccgt cataaaggca attagcgtga cgacaggtac
      361 ggtaagcgaa atggttgcta acactgaacc aaagaacaga ttcatcgcgc gctggacctg
      421 gttgttcaac actgctttta acgcacctaa accttccggc gacagaatca acagagccac
      481 caggaagcca gtaaaggcga caggggcatt catgctgtcg agcaatgtct ccagcgggct
      541 ggcattcatt ttggtgactg caataacggc aatcagatgg ataatcaacc agatagcatg
      601 ccacacgctg ctatgggcag acggtttacc gtgatgcggg tcatcgtcat cactgtcatc
      661 ttcgtgctcg tagacaaaca aactttgatg cgttttggtc tggatcagca aaaatacgcc
      721 atacattgcc gcagaaatta atgctaccag taacgcctga ccggttgaaa aattcgccgc
      781 aggcagagcc attggaaata ccagtacgat tatcgccagg gggaacagcg caattaaata
      841 ctgcttgata ccaaacagat tcatatattg ggtggcaaac ttacgaccgc ccaacaataa
      901 tgaaaagcca accagcccac cggtaacaat cataatgatt gaatagagcg tatcacgcat
      961 tagcgttggc gcggcgtcgc cggttgccat taaagctgaa atcaaactga cttcaagaat
     1021 aaccactgaa aggctaagaa taagcgaacc gtaaggttct cccaggcgat gggctaatac
     1081 gtccgcatga cggacaacac taaaggcgct acttaaaata ccaataagcg caagaagatt
     1141 gatggcaatg accactggta gtgtctggct acttccccac aggaacagca ctaccagcgc
     1201 cagaaccggg aaaataagcg aagtctcctt gtggcgggtt tttaccgcct cttgagcatt
     1261 tgacattatg gttatccctt tgcagatgaa tttatcgaaa atgtaaaaaa taggtaggga
     1321 aaataacaga aagtgtctgg atatcggtaa cattttacga atttttaccc cgctgtcgat
     1381 tttttactca ttggggcata aaaataagta ttacgtttag acaatgtttg ttttagcgtt
     1441 tcttaagaag agtctgacat gaaaattctt atgttttggc aagagtagat attgttgacc
     1501 acacttaatg ttcaactttg taaaaggagt caacgatgcc gtataaaacg aaaagcgatc
     1561 tgccggaaag cgtaaagcac gttctaccgt ctcatgccca ggatatctat aaagaagcgt
     1621 tcaacagcgc atgggatcaa tataaagata aagacgatcg gcgtgatgac gccagtcgcg
     1681 aagaaaccgc gcataaagtg gcctgggctg ctgtgaagca tgaatatgcc aaaggggatg
     1741 atgataaatg gcataaaaaa tcgtaaaacc ggtagcttag ttaaagctat tcgtgcggtg
     1801 ttgccttgca agtggtccgt ggattgcata ttgtcccgtt agtggtttca aaatgagcag
     1861 taaaaatgtc cggaagacac caaaaagttg tcgcagggaa gtatgcagtg gcggaggtgt
     1921 aaggtgataa cgcgtgattt cttgatgaat gccgattgta aaacggcatt tggtgccatt
     1981 gaagaatcac tcttatggtc agcagaacaa cgggcggctt cgctggcggc gacgctggct
     2041 tgtcgacctg atgaggaacc ggtgtggatc ttcggctatg gatcgttgat gtggaatccg
     2101 gcactggagt ttaccgaatc gtgcaccggt acactggttg gatggcatcg cgcattttgc
     2161 ctgcgcctga ccgccgggcg cgggactgcg caccagccgg gacggatgct tgcactgaaa
     2221 gagggcggac gcaccacagg cgtcgcctat cgactgccag aagagacgct ggagcaggaa
     2281 ctaaccttgt tgtggaagcg agagatgatt accggctgtt atctgccaac ctggtgtcag
     2341 cttgatcttg atgatggacg cacagtaaac gccattgtgt ttattatgga tccgcgacat
     2401 ccagaatatg aatctgatac tcgcgctcag gtcatcgcgc cgttgattgc ggcggcgagc
     2461 ggtccgctgg gaaccaacgc acaatacctg ttttcactgg aacaggagct tatcaaactg
     2521 ggaatgcagg acgatgggct gaatgatttg ctggtatcgg taaaaaaact gctggcggag
     2581 aattttccgg atggtgtgtt acgtccggga ttcgcctgag taaacttccc gcatagtggg
     2641 gcgtcagacg ccccctcaaa cattaaaatg tgagcacttt atcggctgac agcgtccatt
     2701 gcgccagttc cacaagagta ccgatttcca ccccatcaat cagaggaagt gtactaatcc
     2761 cgcgcccgtc ggtacaggtt ttgcacaatt tcaccggtac attctgagcg gtaaggatct
     2821 ccagcatttg ctgaatgttg tagccttccc ctggtttttg cccgcgcaac ccggctgtga
     2881 ccgcatcaga catgaggaac agacgcagat ccagattgct ctcctgctct cgtaacgcaa
     2941 tggccagccg caagctgtta aacaaggatt cgctcccgta aggtgcgcca ttggcaacga
     3001 tcacgatttt ttgcattatt tactcctgta ttcagggaat tagacactca tcttctatct
     3061 tactgcttct gcagcgtctg accaatcggt cacattttta aggattttcc tgaaagcgcg
     3121 agaaaatacg acaaaagttg ccagtaatcg ttattcttta aggctatggt ttttcattat
     3181 taccggaagt taccgacgtt ttgagccgtt tcgttcctcg cattatcccg ttttatttac
     3241 tcttgcttgt agcaggcggt acagctaacg cacaatctac cttcgagcaa aaagcggcaa
     3301 atccctttga taataacaat gatggtctgc cggatttagg catggctccc gaaaatcatg
     3361 atggggaaaa acactttgct gaaattgtga aagatttcgg cgaaaccagt atgaatgata
     3421 acgggctgga tactggcgag caggcaaaag ctttcgcatt gggaaaagtc cgcgacgcgc
     3481 ttagtcaaca ggttaatcag cacgtagagt cctggctatc accgtgggga aatgccagtg
     3541 ttgacgtcaa agtggataac gaaggacatt tcaccggcag tcgtggaagc tggtttgtgc
     3601 cgttacaaga taatgatcgt tatctcacct ggagccagct tggtcttact cagcaggatg
     3661 atgggttggt gagcaatgtg ggcgttgggc aacgctgggc gcgcggcaac tggctggtgg
     3721 gttataacac tttttatgac aacttgctgg acgaaaatct tcagcgagcg ggctttggtg
     3781 ccgaagcgtg gggcgaatat ttgcgactat cggcaaactt ttatcagccg tttgctgcat
     3841 ggcatgaaca gacagccacg caggaacaac ggatggcgcg cgggtatgac ctgacagccc
     3901 ggatgcgcat gccgttctat caacacctca ataccagtgt cagcgtagaa cagtattttg
     3961 gtgatcgtgt tgatttgttt aactctggta cgggttatca caatcccgtc gcgttgagtc
     4021 tgggattaaa ttacacccct gtgccattag tcactgtgac ggcccagcat aaacagggtg
     4081 aaagtggcga gaatcaaaat aacctcgggc tgaatcttaa ttaccgcttt ggtgtaccgc
     4141 tcaaaaaaca actttctgcg ggtgaggttg ccgaaagtca gtcgttacgt ggtagtcgct
     4201 atgacaatcc gcagcgaaat aatctaccga ctcttgagta ccgacagcga aaaacgttaa
     4261 cggtgtttct ggcgacaccg ccgtgggatc taaaacctgg cgaaacagtg ccgctgaaat
     4321 tacaaatccg cagtcgttac ggtattcggc aactgatttg gcagggcgat acgcagatat
     4381 taagtttgac gccgggcgca caagccaaca gcgcggaggg ctggacgctg atcatgcctg
     4441 actggcagaa cggggaaagg gcgagcaatc actggcgatt gtctgtggtg gtggaagata
     4501 accaggggca gcgtgtctcc tccaatgaga tcacgctaac gcttgtcgaa ccgttcgatg
     4561 cattgtcaaa cgacgaactg cgctgggaac cgtaatcaga aaatgcgctc ctgatgcacc
     4621 cataccgctg cttccacgcg agacttgagc ttcattttct tcagcatgtg cttgacgtgc
     4681 acttttactg tgctttcggt gatgtccagg cggcgggcaa tcatcttgtt cggcaaaccc
     4741 tgggcaatca gcttgagaat atcgcgctcg cgtggggtta actggttaac atcgcgctca
     4801 gtagtggcac ggttagcacg caagctggcg gccagaacag gcgttaatgc ttcgcttaat
     4861 accatttcgc cagcagctgc ctgatgcaat gctttcagca gatcttccgg ttccatatct
     4921 tttaacagat agccatccgc gccgcgtttc agtgcggtga ccacatcttc ttcatggtta
     4981 gagacgctga ataccacaat gcgccctgag agggactttt cgcgcagttt atccagcgtt
     5041 tccagaccgt tcatgccggg catattgaga tctaacagga tcagatcggg atcaagagac
     5101 tccgccagtt caataccctg ttcgccatta ctcgcttcgc caaccacggt gatatctggt
     5161 gccatactga taagctgttt tacgccagtt cgcagcatcg ggtgatcgtc aatcagcagg
     5221 atagtagccg gttcctgatt actcatgggt atctccttgg acgtctgtga aagttttttc
     5281 gggaataaag gtgaccacca cttcggtgcc acctgattca cgacggcgga cgcggcaatc
     5341 gcctcgtaaa ctttgcgcac ggtcgcgcat tattatcatg ccgtagtgat tgctgcggat
     5401 ggcattttca ggcacgccgc agccgttatc ctgtacggtc agtttgacct gattatcgtt
     5461 ttgcgccacc gtcaccacga cctcactcgc ttgcgaatgt ttgagggcgt tacttaatgc
     5521 ctcacgggca atttgcaaca agtggattgc ctgatgcgaa ggcaccagac gaggcggcaa
     5581 ttgataatcc agcttcaccg ggaagccaaa tttggcgctg tactcttcgc aactcgcctc
     5641 cagcgccgga cgtaatccag gctcggtgag ctgcaagcgg aatgtggtga gcaattcacg
     5701 caactgcgcc caggatgcat tcagttcgtt acggatctga cttaacagtt cgcggctgct
     5761 ttctggcagc gcatcgccct gcatctgtaa acaactcacc tgcatcttca tacaagagag
     5821 agattgggca atagaatcat gcagttcgcg cgcaatggtg gcacgctctt ccatcacgat
     5881 caactgttgc tgacgttcct gatggcgatc caacgccagc gtggcggtga gttgttcaac
     5941 cagggtatcc accagttgtt gttgatcatg gctaagatga cgcccctgcg gcagggtcgc
     6001 cagcaaaata ccgtactgcg tatgagagtc agccagccgc cacttcaggg ttgtgccgcg
     6061 atcgccaacg ggtaatacgc cgcgcgggca gagctggcag cctttatcat cacaagtcat
     6121 atctggctgg caggtaaact cctgatgatt ctcttcatca tcagtatcat aaacccgcag
     6181 ttcgatatca cgtagcaggg ttaaattctg taagccgttg agtaccggtg acaggcgttc
     6241 acacagcggg gcgcgggaat gcaaacggcg gttagcctgc cataaaaaag agaggatctg
     6301 atttttatgc tccagcccgg cggttttctc ctgaacccgc tgctcaagta cggcataact
     6361 ttcggccagt tctgcagaca tattgttcaa cgcagttcca agcatcgcca tttcgttgcg
     6421 cccgctgatg tttgcgcgtt gggtaaaatc gcgatggctg acggcactcg ccattgccag
     6481 cagttgccgc cacggttgta gcagtcgcgc ccgcaaccag ataatagtga acaccagtaa
     6541 aagtgccata aataccgcca ttacccgatg gaccagtacc actgtctcga tgcgcatttc
     6601 cgtggtgcgg tcaaaaccag ataccagctg atcaagcccg gcaacaaact ggctgacatc
     6661 cgctgacacc gtttctcggt tttgtgcacg catcagcgca gggatcagtt cattacgcca
     6721 gtaatcttgt aaaccctgta attgcgccag ttgtccgtct cgttctgctg ctcgagtcaa
     6781 ctcggcgcta aatgccgttt gttccatctc tttaattaag ggcttgtctt tctcgcttaa
     6841 tggcactgcc gccaacagac ggtaactttg catgcgcagc gatcccgctt tgttgatcgc
     6901 atgggcgctg ccctgaacgc cttgcaccag ccagccagaa accgccatcc ctgccagtcc
     6961 aatagcagta gaaagcaaca caataagcgc aacctgatta accagggtga gcggagagag
     7021 acaacgttta agcatgtaaa cctcttcctt caggctttaa atgagcaata accttaatga
     7081 atgtgacgat gcattctgga atggcagtat tctcggctat tggctgaagt atacccatac
     7141 ccggaaagag ttactcctta tttgccgtgt ggttagtcgc tttacatcgg taagggtagg
     7201 gattttacag caccgtgaaa aatctcataa tttttatgaa atcactgtac tcactatggg
     7261 taatgataaa tatcaatgat agataaagtt atcttatcgt ttgatttaca tcaaattgcc
     7321 tttagctaca gacactaagg tggcagacat cgaaacgagt atcagaggtg tctatgagtc
     7381 actcatccgc ccccgaaagg gctactggag ctgtcattac agattggcga ccggaagatc
     7441 ctgcgttctg gcaacaacgc ggtcaacgta ttgccagccg taacctgtgg atttccgttc
     7501 cctgtctgct gctggcgttt tgcgtatgga tgttgttcag cgctgttgcg gtgaacttac
     7561 cgaaagtcgg ttttaatttt acgaccgatc agctatttat gttgactgcg ctgccttcgg
     7621 tttctggcgc gttattacgt gttccatact cctttatggt tcctatcttc ggtggtcgtc
     7681 gctggacggc gttcagcacc ggtattctga ttattccttg cgtctggctg ggttttgccg
     7741 tgcaggatac ctccacgcct tatagcgtct tcatcatcat ctctctgcta tgcggctttg
     7801 ctggcgcgaa cttcgcatcc agtatggcaa acatcagctt cttctttccg aaacagaagc
     7861 agggtggcgc gctgggtctg aatggtggtc tgggaaacat gggcgtcagc gtcatgcagt
     7921 tggttgctcc gctggtggta tcactgtcga ttttcgcagt atttggtagc cagggcgtca
     7981 aacagccgga tgggactgag ctgtatctgg cgaatgcgtc ctggatatgg gtgccgttcc
     8041 ttgccatctt caccattgca gcgtggtttg gcatgaacga tcttgctacc tcgaaagcct
     8101 ccatcaagga gcagttgccg gtactcaaac ggggtcatct gtggattatg agcctgctgt
     8161 atctggcaac cttcggctcc tttatcggct tctccgcggg ctttgcgatg ctgtcaaaaa
     8221 cgcagttccc ggatgttcag attctgcaat acgctttctt cgggccgttt attggtgcgc
     8281 tggcgcgttc tgcaggtggt gcattatctg accgtctggg cggaactcgt gtcacgctgg
     8341 tgaactttat tctgatggcg attttcagcg gcctgctgtt cctgacctta cctactgacg
     8401 ggcagggcgg aagcttcatg gcgttcttcg cggtcttcct ggcgctgttc ctgacagctg
     8461 ggctgggtag tggttccact ttccagatga tttcagtgat cttccgtaaa ctgacaatgg
     8521 atcgcgtgaa agcagaaggg ggttctgacg aacgtgcgat gcgtgaagcg gcaaccgaca
     8581 cggcggcggc gttgggtttc atctctgcga ttggcgcgat aggtggcttc tttatcccga
     8641 aagcgttcgg tagctcgctg gcattaacgg gttcgccagt tggcgcaatg aaagtatttt
     8701 tgattttcta tatcgcctgc gtagtgatta cctgggcggt atatggtcgg cattctaaaa
     8761 aataaaccgt tactcgtcat acttcgggtt acatgtgctg cggctgcgtt cattcacccc
     8821 agtcacttac tttagtaagc tcctgggatt cattcacttg ccgccttcct gtaaaccgaa
     8881 ttatatagag taaaatattt gattatcctt tgcgcggcat gatgtcgcgc tttttttatg
//
LOCUS       DAPHDW010000165         8828 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.168, whole genome shotgun sequence.
ACCESSION   DAPHDW010000165 DAPHDW010000000
VERSION     DAPHDW010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8828)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8828)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8828
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.168"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1145
                     /locus_tag="RH057_003919"
     CDS             <1..1145
                     /locus_tag="RH057_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001736301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HDV3130218.1"
                     /translation="FIAGLLHLNSIFPNLEKAQAIFTPVMNGSVNIMSIMIAFLVARN
                     MAISYEQDDLLCGLTAIGAFFIVYTPYQMIDGQAFLTTKYLGAQGLFVAVIVALITSE
                     IFCRLARNPKITITMPAAVPPAVARSFKVLLPIFFVMVFFSALNYCLALISPAGLNDL
                     IYTLIQTPLKHMGTNIFAVIILGAVGNFLWVLGIHGPNTTSAIRETVFSEANLENLSW
                     AAQHGTTWGAPYPITWTSINDAFANCGGSGMTLGLLLAIFIASKRAEYRDLAKMSFIP
                     GIFNINEPIMFGLPIVLNPIMMVPFIMVPIVNCAIGYFFVSMEIIPPVAYAVPWTTPG
                     PLIAFLGTGGNWLALLVGFLCLGVATMIYLPFVIAANKVNNMTTNG"
     gene            1173..1484
                     /locus_tag="RH057_003920"
     CDS             1173..1484
                     /locus_tag="RH057_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HDV3130219.1"
                     /translation="MFADEELVMELLVNAGQARSDAMEAIRCAGQKDWQGATQLMASS
                     ESACLQAHKIQTALISQDEGCGKIKVNLILIHAQDHLMNAILCQDLAREIISLRKELH
                     A"
     gene            1540..3213
                     /locus_tag="RH057_003921"
     CDS             1540..3213
                     /locus_tag="RH057_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate porin"
                     /protein_id="HDV3130220.1"
                     /translation="MNIKTLNVSLLSFSIITALFPLNAMATKLTIEQRLELLENELSQ
                     NKQELKATQNELGVYKSRLSTLQKSITENKYKSASLAEISATSPVADNIKNENGEQNS
                     FTAAHTINGSQQVAVIESKGDKTTIESVTLKDISKYIKDDIGFSYQGYFRSGWGTGNH
                     GSPQTYAAGSLGRFGNEMSGWFDLTLNQRVYNQDGKTANAVVTYDGNVGEQYNDAWFG
                     DSANENIMQFSDIYLTTRGFLPFAPEADFWVGKHKLPQYEIQMLDWKTLTTDVAAGVG
                     IENWALGVGLFDMSLSRDDVDVYSRDFSRTSQMNTNSVDVRYRNIPLWDDATLSLMAK
                     YSAPNKTDQQQDNENDNSYFEMKDSWMLTSVLRQNLQRDTFNEFTLQVANNSYASSFA
                     SFSDASNTMAHGRYYYGDHTNGIAWRLISQGEMYLTDNIIMANALVYSHGEDVYSHES
                     GAHSDFDSIRTVIRPAWIWNTWNQTGLELGWFKQQNKTQQGVTLNESAYKTTLWHALK
                     VGESILGSRPEIRFYGTYINILDNELSNFKFNENSKDEFMAGIQAEVWW"
     gene            3238..4677
                     /locus_tag="RH057_003922"
     CDS             3238..4677
                     /locus_tag="RH057_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV3130221.1"
                     /translation="MSGFKKGFLWGGAVAAHQLEGGWNEGGKGISIADVMTAGAHGVP
                     REVTEGVIDGLNYPNHEAIDFYHRYKTDIQLFAEMGFKCFRTSIAWTRIFPQGDEQEP
                     NEEGLQFYDDLFDECLKQGMEPVVTLSHFEMPYHLVTKYGGWRNRKLIDFFIRFASTV
                     FTRYKEKVKYWMTFNEINNQVNFSESLCPFTNSGILYSPEEDINEREQIMYQAVHYEL
                     VASALAVQTGKSINPEFNIGCMIAMCPIYPLTCAPNDMMMATKAMHRRYWFTDVHARG
                     YYPQHMLNYFARKGFNLDITPEDNAILASGCVDFIGFSYYMSFTTQFSPDNPQLDYVE
                     PRDLVSNPYIDTSEWGWQIDPAGLRYSLNWFWDHFQLPLFIVENGFGAVDQRQADGTV
                     NDHYRIDYFASHIREMKKAVVEDGVDLIGYTPWGCIDLVSAGTGEMKKRYGMIYVDKD
                     NEGKGTLERIRKASFYWYRDLIANNGENI"
     gene            complement(4734..4952)
                     /gene="marB"
                     /locus_tag="RH057_003923"
     CDS             complement(4734..4952)
                     /gene="marB"
                     /locus_tag="RH057_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HDV3130222.1"
                     /translation="MKPLSSAIAAALILFSAQGVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(4984..5367)
                     /gene="marA"
                     /locus_tag="RH057_003924"
     CDS             complement(4984..5367)
                     /gene="marA"
                     /locus_tag="RH057_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HDV3130223.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            complement(5387..5821)
                     /gene="marR"
                     /locus_tag="RH057_003925"
     CDS             complement(5387..5821)
                     /gene="marR"
                     /locus_tag="RH057_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HDV3130224.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            6033..6698
                     /gene="marC"
                     /locus_tag="RH057_003926"
     CDS             6033..6698
                     /gene="marC"
                     /locus_tag="RH057_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HDV3130225.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            complement(6723..7913)
                     /gene="ydeA"
                     /locus_tag="RH057_003927"
     CDS             complement(6723..7913)
                     /gene="ydeA"
                     /locus_tag="RH057_003927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HDV3130226.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALITLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(8063..8828)
                     /gene="yneK"
                     /locus_tag="RH057_003928"
                     /pseudo
     CDS             complement(8063..8828)
                     /gene="yneK"
                     /locus_tag="RH057_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416044.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="putative protein YneK"
BASE COUNT         2416 a         1895 c         2121 g         2396 t
ORIGIN      
        1 gttttattgc cggattactg catctgaata gcattttccc caaccttgaa aaagcacaag
       61 ctatttttac tccggtaatg aatggttctg taaatatcat gtcaattatg attgctttcc
      121 tggtcgccag gaatatggcg attagctatg agcaagatga tcttttatgc ggattaacgg
      181 caataggagc attttttatt gtttatacgc catatcagat gatagatggg caagcattcc
      241 tgacgaccaa atatctcggc gcgcaggggt tgtttgttgc tgttatcgtt gcattgatca
      301 ccagtgaaat attttgtcgc ttagctcgaa acccaaaaat caccataacg atgccggcag
      361 ctgtaccgcc tgcggtagcg cgttcattta aagttttatt gccaatattt tttgtcatgg
      421 tgttcttttc cgcacttaat tattgcctgg cactgatatc cccggcagga ctaaacgatc
      481 ttatttacac attaatccag acgccgctca aacatatggg aacgaatatc tttgcggtaa
      541 ttatcctggg ggctgtgggt aatttcctgt gggtgctggg gatccacgga cctaatacca
      601 catcggcaat tcgagaaacc gttttttctg aggctaatct ggagaatctc tcctgggccg
      661 ctcaacatgg cactacctgg ggcgcgccat atccgattac ctggacttct attaatgatg
      721 cattcgccaa ctgcggcggc tcaggtatga cgttggggtt attgttggct atttttatcg
      781 cttctaagcg tgcggaatac cgtgatctgg caaaaatgtc atttatcccc ggtattttca
      841 atatcaatga accgataatg tttggccttc ctattgtact taaccccatc atgatggtgc
      901 cgtttattat ggttcccatt gttaactgtg ccattggtta cttctttgtt tcgatggaaa
      961 ttattccacc ggttgcttat gccgtgccct ggactacgcc cggaccttta attgctttcc
     1021 tcggaaccgg ggggaactgg ctggctttac tggttggttt tttatgttta ggtgtggcga
     1081 caatgatcta tttacctttt gttattgccg ccaacaaagt caataacatg acaactaacg
     1141 gataattcca tttacccagg aaggagattg tgatgtttgc agatgaagaa cttgttatgg
     1201 agttactggt caacgcgggg caggcacgtt ctgacgcgat ggaagccata cgttgtgccg
     1261 ggcaaaaaga ctggcagggc gcaactcagc taatggccag ttctgaatct gcctgtcttc
     1321 aggcacataa gattcagacc gcattaatta gtcaggatga aggctgtggc aaaataaaag
     1381 ttaatttaat tctgattcat gcacaggatc atttgatgaa tgcaatatta tgccaggatt
     1441 tagccaggga aattatttca ttaagaaaag aactccatgc ctgatttttt cctccagtgc
     1501 gatagctgga ggaaattatt atactcttaa ggataagtca tgaatataaa gacgcttaac
     1561 gtcagccttt tgtctttttc tattattaca gcattgtttc cattgaacgc gatggcaaca
     1621 aaattaacca tagagcagcg ccttgaattg cttgaaaatg aattgtcgca aaataaacaa
     1681 gagctgaaag caacacagaa tgaactggga gtatataaat cccgactttc gaccttacaa
     1741 aaaagcatca cggaaaacaa atataaatca gcctcgcttg ctgaaatatc agcgacatct
     1801 cccgtcgctg ataacatcaa aaatgaaaac ggtgaacaga actcgtttac cgcagcgcat
     1861 actataaatg gatcgcagca agttgccgtt attgaaagta aaggcgacaa aaccactatc
     1921 gaaagcgtga ccctgaaaga tatcagtaaa tatataaagg atgatattgg gttcagctat
     1981 caggggtact tccgctcagg ctggggtact ggtaatcacg gctcaccaca aacttatgca
     2041 gcgggttctc tgggacgttt tggtaatgag atgagcggtt ggtttgacct gaccttaaat
     2101 cagcgtgttt ataatcagga cggtaaaacg gcaaatgcgg tcgttaccta tgatggcaac
     2161 gtgggtgagc agtataacga tgcctggttt ggtgacagtg ccaatgaaaa tatcatgcag
     2221 ttcagcgata tttacctgac aacgcgaggt tttttaccct tcgcaccaga ggcagacttc
     2281 tgggtcggca aacataaact cccgcaatat gagatccaga tgctggactg gaaaacctta
     2341 accacggatg tcgccgcggg tgtggggatt gaaaactggg cacttggtgt agggctgttt
     2401 gatatgtcct taagccgtga tgatgtcgat gtttactctc gtgatttttc gcgtaccagc
     2461 cagatgaata ccaattctgt ggatgttcgt tatcgcaata tcccgttatg ggatgatgcg
     2521 acattatcat taatggctaa atattccgca cctaataaaa cggatcaaca acaagataac
     2581 gaaaatgaca acagttattt tgaaatgaaa gatagctgga tgctgacttc tgttttacgg
     2641 caaaacctgc aacgcgatac gtttaatgaa tttacgttac aggttgccaa taattcctat
     2701 gccagcagtt ttgccagttt ctcagatgcc agtaatacga tggcgcatgg tcgctattac
     2761 tatggtgatc ataccaatgg gatcgcctgg cgtttaatct ctcagggcga gatgtatctt
     2821 actgacaata ttattatggc taacgcgctt gtctattctc acggcgaaga tgtttatagt
     2881 catgaaagtg gtgctcatag tgattttgac agtattcgca ctgtaataag accggcctgg
     2941 atctggaata catggaatca gacggggctt gaattaggct ggtttaagca acagaacaaa
     3001 actcagcagg gtgtaacact aaatgaatcg gcttataaaa cgacactctg gcatgcattg
     3061 aaagtgggtg aaagcatttt aggttcacga ccagaaattc gcttctatgg aacgtatatc
     3121 aatattctgg ataacgaatt atcgaatttt aagtttaatg agaacagcaa agacgagttt
     3181 atggccggca tccaggcgga agtctggtgg taatctattt tagttaagga gttgtgtatg
     3241 tcaggattta aaaaaggttt tttatggggc ggtgcggtag ccgcgcatca gttggaaggt
     3301 ggctggaatg aaggaggaaa aggcatcagc atcgctgatg tgatgactgc tggcgctcac
     3361 ggggtgccgc gtgaagtgac agaaggcgtt atcgacgggc ttaattatcc caatcatgaa
     3421 gcaattgatt tttatcatcg ctataaaaca gatattcagt tatttgccga gatgggattc
     3481 aaatgctttc gaacttccat tgcctggaca cgaatctttc cgcaaggtga cgaacaggag
     3541 ccgaatgaag agggtttaca attttatgat gatctgttcg atgaatgcct taagcaggga
     3601 atggaacctg tggtgacgct ttcacatttt gagatgcctt atcatctggt gacaaaatat
     3661 ggtggctggc gaaaccgtaa actgatcgac ttttttatcc gcttcgcatc aacggtcttc
     3721 acgcgctata aagaaaaagt aaagtactgg atgacgttta acgaaatcaa taatcaggtg
     3781 aatttcagcg aaagcctgtg tccatttact aattccggta tcttgtattc gccagaggaa
     3841 gatatcaatg agcgcgaaca aataatgtac caggcggtac attacgagtt agttgccagt
     3901 gccctggcgg tacagactgg aaaatcgatc aatcctgaat ttaacattgg ctgtatgatc
     3961 gccatgtgtc ccatctatcc tctgacgtgt gcacccaacg atatgatgat ggccacgaaa
     4021 gcaatgcatc gtcgttactg gtttactgat gttcatgctc gtggatatta tccacaacat
     4081 atgctgaatt actttgccag gaaaggattc aacctcgata tcacaccaga agataacgcg
     4141 attcttgcca gtggttgtgt cgactttatc ggctttagct actacatgtc ttttacgaca
     4201 caattttcgc ccgataaccc gcaactggat tatgttgaac cacgagattt ggtcagcaac
     4261 ccttatatcg atacatccga atggggatgg caaattgatc cggcagggct acgttattca
     4321 ctcaactggt tctgggatca tttccagttg ccgctgttta ttgtcgaaaa tggatttggt
     4381 gcggttgacc agagacaagc tgacggcacg gtgaacgatc actatcgcat tgattacttt
     4441 gcttcccata ttcgggaaat gaaaaaagcc gtagttgaag atggtgttga cttaattggc
     4501 tacaccccgt ggggctgcat tgacctggtt tctgccggaa caggggaaat gaaaaagcgc
     4561 tacggaatga tttatgtcga taaagacaac gaagggaagg gaacgctgga acggatacgt
     4621 aaagcgtcgt tttactggta tcgggatctc atcgccaaca atggcgaaaa tatttgagac
     4681 ttgaggaaga tattacggag atgtcaggct ctgtgatcag agcctgacaa taactacata
     4741 gcgtgttgat tataataggg cacgccgagc gcatccgact tatcactgcc agtacccatg
     4801 tgatttaaat caaacggtgg ttgttcctgc gatgggggaa caaccacgac attggcacaa
     4861 gaagtaacaa ctggctgcgt ggtttgttcc gcaacgccct gcgcggaaaa gagaataagc
     4921 gcagctgcta ttgcggatga aagtggtttc atgattgcct cagtgacgtt gtcacgttat
     4981 caactagctg ttgtaatgat ttaatggatg taaaaagcgc gattcgccct gcatattggt
     5041 catccggtat ttatgcggcg gaacatcaaa gtaatttttg aaggttcggg tcagagtttg
     5101 ttgcgactcg aagccatatc gttctgccag atagagtatc ggctcgttac tttccttcag
     5161 cttttgcgcg atttccgtca tcttacggct gcggatgtat tggcctaatg aatgaccggt
     5221 ttctttttta aacatccgtt gcaggtgcca tttagagtaa cctgaacgct ctgacacttt
     5281 ctccagtgac agtggcgatt ccaggttgtc ctcgatccag tccaaaatgc tatgaatggt
     5341 aatagcgtca gtattgcgtc tggacatcgt catacctctt ttttgtttac ggcaggactt
     5401 tcttaagcaa atgctcaagt gttgccactt cgtccgccgt caggtttttt gttaattctt
     5461 gatgcaggtc ctggccaact aattgatggc attgttcgca tattgccgcg ccgctggtgg
     5521 taagttttac cagtacgccg cgcttatcat tcgggttcgg caacctttca acccagcctt
     5581 tacagaccag gcgatccagc atacgggtca gtgctccaag gtcgacagac aacacttttt
     5641 tcagttcaac cggagtaata cacgccgcgc agcggataga gcagagcacc ttaaactgtg
     5701 ccgcggtaat atccagcgga gacagatact cgttaagcag acgatctttc ttctgattaa
     5761 ccatatggat taagcgaccc aatggaataa tttcattgaa cagatcgctg gtacttttca
     5821 cattagttgc cctggcaagt aattagttgc aggggataat attgcccagg caagtataag
     5881 tcaaataaat gaattgaccg atgccacgtt ttgctaaatc ggttcaagtt ttggtcaatg
     5941 cgatgcaagg ttagctaaag agtgacactt tatccttgtt taccccttat acttttcgct
     6001 gataacccag atacacagga taacaaccac taatgttaga tttgtttaaa gcaattggcc
     6061 tggggctggt ggtgttactg ccgttagcta acccattaac aactgtcgcg ttatttcttg
     6121 gcctggcagg caacatgaac agtgccgaac gtaatcgtca gtcgttgatg gcctcggtgt
     6181 acgtatttgc catcatgatg gtggcgtatt acgccgggca actggtgatg gatacatttg
     6241 gcatttcgat tcccggtctg cgaattgcag gcggcttaat cgtcgcgttt atcggttttc
     6301 ggatgctctt tccgcaacag aaagcgattg attcaccgga ggcgaaaagc aagtcggaag
     6361 agctggaaga tgaacccagt gccaatattg cctttgtgcc gctagcaatg ccaagtaccg
     6421 ctggtccggg aaccatagcg atgattatta gctcggcgtc cacagtgcgt cagagttcaa
     6481 ccttcgctga ttgggtactg atggttgcgc cgccgctgat ctttttcctg gtggcggtta
     6541 ttttgtgggg aagcctacgc agctccggcg caattatgcg gctggtgggc aagggtggca
     6601 ttgaagcgat ctcccgtctg atgggattcc tgctggtatg tatgggtgtg cagtttatta
     6661 ttaacggcat cctggaaatc attaaaacgt accattaaaa aaggcccgaa tgggcctttc
     6721 aactattgcg tctgttcttc gagtgtcact ggccagcggc gaaatataat gattgaccaa
     6781 attaacgcgg caaaagcagg caccgcgccc acataaccaa tcatcgacat tgaccagtgc
     6841 aaactcacct gattacctac caacgcaccc gccccgattc caatattaaa tatcccggag
     6901 aatagcgcca tcgcgacgtc ggtagcatct ggtgccagcg ccagcacttt aacctgcata
     6961 ccaagcccga tgatcatcat cgcgatcccc cagaaaatac tcagcacccc gaggtgtatt
     7021 tcactgttcg ccgcaggtaa cagcaatgcc aggcacacca gcaacagcgc aatcgccgta
     7081 ctcaccaacg cagacgcata ctgattaccc agtttaccga aaatcacgct gccaataatg
     7141 cccgcaccac cgagtaataa cagtaatgcc gtggcaaagt tggcgctgaa tcccgcaatg
     7201 ttttgtacaa aaggctcgat atagctgtat gccgtgtaat gggcggtgac aaccaccaca
     7261 gttaacaaat aaatgctcat caatgccggg cggcggaaca atagcgggag gcttttcagt
     7321 gaaccggaat gctcactggg cagtaaggga agtaacttaa tcaggcacaa aagggtgata
     7381 agcgccccaa taccaatcgc gaagaaggtc attcgccaac cgaaatactg gcccacaatg
     7441 cgcccgagag gtaaacctaa gaccatcgcc agtgctgtac cggtggcaat taaactcaat
     7501 gcctgtgctc gcttcccggc cggagccata cggatcgcca gagacgccgt aatcgaccag
     7561 aaaatcgcat gtgcaaaagc cacaccaatg cgactgatca ccagaacggt aaagctccac
     7621 gacaaaaacg acagtacgtg gctggcaata aacaccacaa acaggcagat cagtaatttg
     7681 cgccgttcaa cctgactggt cattaacata aaaggcaatg acattagcgc tactacccat
     7741 gcgtaaatgg tcaacatgat gccgacctga gcggtttgca tgtgaaaact ttgcgcaatg
     7801 tcagagagca ggccaacagg gacaaattcg gtggtgttga agatgaaggc ggcgactgcc
     7861 agcgtaacga cccgtagcca cgccactttg cgggaaacag tgtttgttgt cataacagat
     7921 tatcgggtga gttgcgaaaa agtgatgaaa tgatcttaaa acggttaatg atgtgaatac
     7981 aatgcatttg tgatctggat cgcgttttcc gtttctctga cgctgattct ttcacgcgtc
     8041 agagagtcgg taacatcacg gtttaatgac gttcagggag gtctggcgtt ttggaggttg
     8101 cgggtatagg tggtctgaca ggggagcgag gcctgttttt caccaggtct tcgtgagtaa
     8161 cttttggcgg cgtttcattt tttacaaaca gtttgagggt cctgcgcgcc atatgtttga
     8221 taattttatc atcacttaat gatgctggca tcaacacgtc attcagatca ctttcattgg
     8281 gattcataaa ttggtgatag tgcaataaca tattggcgaa aaataaaaaa aagtcctgaa
     8341 aatgaggaga ttcttgttct gctaaaaggg ataaaaagca gatgcagact tcgggtagta
     8401 catgtttgtt agaacctaat gaatccatca ggctttgcaa taaatcctct tttaattcgc
     8461 cgtctgttat gtcgagaatg gcggtgatca agttaggatc tttattgacg aaccatctgt
     8521 gaacctcaga gttactccat aatcggggga gtatgacaaa attaccgcga gagataaaat
     8581 tcaggacaag caaatcaaac tggcgtaagt aatctgcagc ggtatggcga tccggaggca
     8641 ggggttcaat atgtggtgta tgcgctcttt tgatgggaac gccaaggttg atcattggaa
     8701 attgttcaat aaaatcattc agaaacagaa atagcacacg ctcgacgccg gtctgaaata
     8761 cgcgccccat agtgacaggc tttgggttgg gagcgcaaaa atattaattt ctccatcatg
     8821 gcgaaaat
//
LOCUS       DAPHDW010000166         8812 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.169, whole genome shotgun sequence.
ACCESSION   DAPHDW010000166 DAPHDW010000000
VERSION     DAPHDW010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.169"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            13..837
                     /gene="gadX"
                     /locus_tag="RH057_003929"
     CDS             13..837
                     /gene="gadX"
                     /locus_tag="RH057_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HDV3130227.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTDRSAEGIRL"
     gene            1205..1933
                     /gene="gadW"
                     /locus_tag="RH057_003930"
     CDS             1205..1933
                     /gene="gadW"
                     /locus_tag="RH057_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HDV3130228.1"
                     /translation="MTHVCSVILIRRSFDIYHEQHKISLHNESIVLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2296..5409)
                     /locus_tag="RH057_003931"
     CDS             complement(2296..5409)
                     /locus_tag="RH057_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HDV3130229.1"
                     /translation="MANYFIDRPVFAWVLAIIMMLAGGLAIMNLPVAQYPQIAPPTIT
                     VSATYPGADAQTVEDSVTQVIEQNMNGLDGLMYMSSTSDAAGNASITLTFETGTSPDI
                     AQVQVQNKLQLAMPSLPEAVQQQGISVDKSSSNILMVAAFISDNGSLNQYDIADYVAS
                     NIKDPLSRTAGVGSVQLFGSEYAMRIWLDPQKLNKYNLVPSDVISQIKVQNNQISGGQ
                     LGGMPQAADQQLNASIIVQTRLQTPEEFGKILLKVQQDGSQVLLRDVARVELGAEDYS
                     TVARYNGKPAAGIAIKLATGANALDTSRAVKEELNRLSAYFPASLKTVYPYDTTPFIE
                     ISIQEVFKTLVEAIILVFLVMYLFLQNFRATIIPTIAVPVVILGTFAILSAVGFTINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVIAEDKLPPKEATHKSMGQIQRALVGIAVVLS
                     AVFMPMAFMSGATGEIYRQFSITLISSMLLSVFVAMSLTPALCATILKAAPEGGHKPN
                     ALFARFNTLFEKSTQHYTDSTRSLLRCTGRYMVVYLLICAGMAVLFLRTPTSFLPEED
                     QGVFMTTAQLPSGATMVNTTKVLQQVTDYYLTKEKDNVQSVFTVGGFGFSGQGQNNGL
                     AFISLKPWSERVGEENSVTAIIQRAMIALSSINKAVVFPFNLPAVAELGTASGFDMEL
                     LDNGNLGHEKLTQARNELLSLAAQSPDQVTGVRPNGLEDTPMFKVNVNAAKAEAMGVA
                     LSDINQTISTAFGSSYVNDFLNQGRVKKVYVQAGTPFRMLPDNINQWYVRNASGTMAP
                     LSAYSSTEWTYGSPRLERYNGIPSMEILGEAAAGKSTGDAMKFMADLVAKLPAGVGYS
                     WTGLSYQEALSSNQAPALYAISLVVVFLALAALYESWSIPFSVMLVVPLGVVGALLAT
                     DLRGLSNDVYFQVGLLTTIGLSAKNAILIVEFAVEMMQKEGKTPIEAIIEAARMRLRP
                     ILMTSLAFILGVLPLVISHGAGSGAQNAVGTGVMGGMFAATVLAIYFVPVFFVVVEHL
                     FARFKKA"
     gene            complement(5434..6591)
                     /gene="mdtE"
                     /locus_tag="RH057_003932"
     CDS             complement(5434..6591)
                     /gene="mdtE"
                     /locus_tag="RH057_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtE"
                     /protein_id="HDV3130230.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSRQNVLLVPQEGVTHNAQGKATALILDKDDVVQLREIE
                     ASKAIGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
     gene            6651..6929
                     /locus_tag="RH057_003933"
     CDS             6651..6929
                     /locus_tag="RH057_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130231.1"
                     /translation="MRDLQTSGIVGLSASKVGYRYSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNKVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(6930..7457)
                     /gene="gadE"
                     /locus_tag="RH057_003934"
     CDS             complement(6930..7457)
                     /gene="gadE"
                     /locus_tag="RH057_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HDV3130232.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(8256..>8812)
                     /gene="hdeD"
                     /locus_tag="RH057_003935"
     CDS             complement(8256..>8812)
                     /gene="hdeD"
                     /locus_tag="RH057_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HDV3130233.1"
                     /translation="ATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFVSGDILS
                     TVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPELGIFAIA
                     AFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVSVTLVST
                     LVGIELIFSAASLFSFASLFVKQQ"
BASE COUNT         2357 a         2052 c         2063 g         2340 t
ORIGIN      
        1 attaagtcaa ctatgcaatc attacatggg aattgtctaa ttgcgtacgc aagacataaa
       61 tatattctca ccatggttaa tggtgaatat cgctatttta atggcggtga cctggttttt
      121 gcggatgcaa gccaaattcg agtagataag tgtgttgaaa attttgtatt agtatcaagg
      181 gatacgcttt cattatttct gccgatgctc aaggaggagg cattaaatct tcatgcacat
      241 aaaaaaattt cttcattact cgttcatcac tgtagcagag atattcctgt ttttcaggaa
      301 gttgcgcaac tatcgcagaa taagaatctt cgctatgcag aaatgctacg taaaagagca
      361 ttaatctttg cgttgttatc tgtttttctt gaggatgagc actttatacc gctgcttttg
      421 aacgttttgc aaccgaacat gcgaacacga gtttgtacgg ttatcaataa taatatcgcc
      481 catgagtgga cactagcccg aatcgccagc gagctgttga tgagtccaag cctgttaaag
      541 aaaaaattgc gcgaagaaga gacatcatat tcacagttgc ttactgagtg tagaatgcaa
      601 cgtgctttgc aacttattgt tatacatggc ttttcaatta agcgagtcgc agtgtcctgt
      661 ggatatcaca gcgtgtcgta tttcatttac gtctttcgaa attattatgg gatgacgccc
      721 acagagtatc aggagcgatc ggcgcaggga ttgccgaacc gtgactcggc ggcaagtatt
      781 gttgcgcaag gaaattttta cggcactgac cgttctgcgg aaggaataag attatagaat
      841 tttactcaga cataaaaaaa acccggcata ggggaccggg aagaggatag tctgccgtct
      901 ccagactaat aaaccgttat aacactccct gttggcacgg gaaactttgt gctctcagta
      961 agttaaatat aacttttact ggaaataaga tcagccattt ttttataaac ataagctata
     1021 cgcagtgcga aaatatattc gtgctgcatt tacttattat caattaactg ttatgcaaaa
     1081 ctactttgtg gataaatttt ggtcctacca aatctggcag ttttttcgct aagaaacagt
     1141 ctggcaacat ttcattagta tactgaaatt gaaataatcg cagtatgaaa tataagggat
     1201 aatcatgact catgtctgct cggtgatcct cattcgtcgt tcattcgata tttatcatga
     1261 acagcataaa atatcgctgc ataacgagag tatcgtgctg ctggagaaaa atttggcaga
     1321 cgattttgcg ttttgttcac cggatacgcg acgactggat atcgatgagc tgacagtttg
     1381 ccattactta caaaatattc gtcagctacc acgcaattta gggttacata gcaaagaccg
     1441 tctattaatt aaccagtcac cccccatgcc gctggtgacg gcgatttttg atagcttcaa
     1501 tgaatccggg gtaaattcac cgatactgag caatatgctc tacctttcct gtttatcgat
     1561 gttttctcat aagaaagaac tgatcccctt acttttcaat agcatcagca ctgtttcagg
     1621 aaaagttgaa cgccttatta gctttgatat cgccaaacgt tggtatctgc gcgatatcgc
     1681 ggaaagaatg tataccagcg agagtctcat caaaaaaaag ttgcaggatg aaaatacctg
     1741 tttcagtaaa atattactcg cttccaggat gtcgatggcc agacgattac tcgagttacg
     1801 tcaaattcct ctgcatacta ttgcagaaaa atgtggctat agcagtacgt cgtactttat
     1861 aaacacattt cgacaatatt atggtgtaac gccacatcag tttgcgcaac attcgccagg
     1921 taccttttcc tgacatattt tgcatttgaa tattggtcag gatctcacac ctgcttcatg
     1981 tgaaactctt ccctgatgat ttctgccggg ctaccggcta gttctctttc gcagtttgcc
     2041 aggcggtata ggtattttca ggaggaaaag actcggcatg tttgggatta ttaagctgac
     2101 aattcatacg attacgggaa tgtgggtcag tattgtgctg ttcaaattga tgacgaatgg
     2161 ctggagtggt ttctactttc agtgctgtgt actaagcctg gtattcctga cggttagctg
     2221 gttgttgagt ggtgaatggt tagcaggaaa gagtaaggct gaaccttcac gttcaacctt
     2281 actctcattt acacgttacg cttttttaaa gcgggcaaag agatgttcca ctacaacgaa
     2341 aaagaccgga acgaagtaaa ttgccagcac tgttgcggca aacatcccgc ccatcacgcc
     2401 ggtacctacc gcgttttgcg cgccagaacc ggcaccatga ctgataacca gcggcagcac
     2461 gccgagaata aaggccagag aggtcatcag gattgggcgt aaacgcatcc gcgccgcttc
     2521 gatgattgcc tctatcggcg ttttcccttc tttttgcatc atctcaacgg caaattcgac
     2581 aatcaggatg gcgttcttgg cggaaagccc gatggtggtc agcaaaccaa cctggaagta
     2641 gacgtcatta cttaagccgc gcagatcggt ggccagtaat gcgccaacga cgcctaacgg
     2701 aacaaccaac atcaccgaga acggaattga ccagctctca tagagtgcgg cgagggcgag
     2761 gaacaccacg accagtgaaa tggcatacag tgcaggagcc tggtttgagg ataacgcttc
     2821 ctgatacgac agtccggtcc atgagtagcc gacgcctgcc ggaagtttag cgaccaggtc
     2881 tgccataaat ttcatagcgt caccggtact tttcccggcc gccgcttcac ctaaaatctc
     2941 cattgacggg atgccgttgt agcgttccaa tcgcggtgaa ccataggtcc attcggtaga
     3001 cgagtaggca gaaagcggtg ccatcgtgcc agaggcgttg cgtacatacc attggttgat
     3061 gttatccggc aacatacgga acggcgtgcc tgcctggaca tacacttttt tcacccgccc
     3121 ctggttgagg aagtcgttca cgtagctgct gccgaaggcg gtggaaattg tctggttgat
     3181 atcagacagc gccacaccca ttgcctcagc tttcgcggcg ttgacgttca ctttgaacat
     3241 cggcgtatct tccaggccgt tcgggcgtac cccggtgacc tggtcaggcg attgcgctgc
     3301 cagtgataac agctcgtttc gtgcctgggt tagtttttcg tgccccaggt taccgttgtc
     3361 cagcagttcc atatcaaaac ctgacgcggt acccagttca gccaccgcgg gtaagttgaa
     3421 cgggaagacg acggctttat tgatactgct taacgcaatc attgcccgct gaatgatcgc
     3481 ggtaaccgag ttttcctcac cgacacgttc agaccacggc ttgagactga taaacgccag
     3541 gccgttgttt tgcccctggc cgctgaagcc aaagccgcca acggtaaaca ccgactgaac
     3601 attatctttc tcttttgtaa gataataatc cgtcacctgt tgcagcactt tcgtggtgtt
     3661 aaccatggtg gcaccggaag gtaactgcgc ggtggtcata aataccccct gatcctcttc
     3721 tggtaagaaa gaggtcggtg tacgcaggaa cagcaccgcc atcccggcgc aaatcagcag
     3781 gtagaccacc atgtagcgac cggtacaacg caacagcgag cgggtgctat cggtatagtg
     3841 ctgagttgat ttttcaaaca gcgtgttgaa gcgtgcgaac agggcgttag gtttgtgacc
     3901 gccttccggc gcggctttca gaatggtggc gcacagggca ggggtcaggc tcattgccac
     3961 aaatactgaa agcagcatgg aggagatcag cgtgatggag aactggcggt agatctcccc
     4021 ggttgcgccg ctcataaagg ccatcggcat aaacactgcg gaaagaacaa cagcaatacc
     4081 gaccagcgca cgttggatct gccccatcga tttatgcgtc gcttccttcg gtggtagctt
     4141 atcttccgca atgacacgct cgacgttctc caccaccacg atggcgtcat ccaccagtaa
     4201 ccctatcgcc agcaccatcc cgaacatagt taacgtgttg atggtgaaac cgaccgccga
     4261 caagatcgca aacgtcccga gaataaccac cggtacggca atcgtcggga tgattgtggc
     4321 acggaaattc tgcaaaaaca gatacatgac caggaagacg aggatgatag cctcaaccag
     4381 tgttttaaaa acttcctgaa tagaaatttc gataaacggc gtggtgtcgt aaggataaac
     4441 cgtcttcaga cttgcgggga aataggctga taagcggttc agctcctctt tgactgcccg
     4501 cgaggtatcc agggcgtttg ctccggtagc cagtttgatg gcgatcccgg cagcaggttt
     4561 gccgttatag cgcgccacgg tggaataatc ttccgctcca agttcgacgc gggcgacatc
     4621 acgcagcagc acttgcgaac catcttgctg aactttcaac aggattttgc caaattcttc
     4681 cggcgtttgc agacgcgtct gcacaatgat cgaggcgttt agttgctggt ctgccgcctg
     4741 tggcatgcca cccagttgac caccggaaat ctggttgttt tgcaccttaa tctgggaaat
     4801 aacatcggaa ggtaccagat tgtatttatt gagtttttgc gggtccagcc agatacgcat
     4861 ggcgtactcg gaaccaaaga gctgtacgct accaacgccc gcggtacggc ttagcgggtc
     4921 tttgatatta gacgctacat agtccgcgat atcgtactgg ttgaggctgc cgttatcaga
     4981 aataaacgcc gctaccatca gaatattact gctcgactta tcaacgctaa tcccctgctg
     5041 ctgcaccgct tcaggtaatg aaggcatagc gagttgcagt ttattttgca cctgaacctg
     5101 tgcgatatca ggagatgtcc cagtctcgaa ggtcagagtg atagaggcat tacccgccgc
     5161 atcactggtt gaagacatgt acatcaggcc atcaagccca ttcatatttt gctcaatcac
     5221 ctgagtgacc gagtcttcta ccgtttgcgc atcggcacct ggataggtag cgctgacggt
     5281 aatggtcggt ggcgcaatct gcggatactg cgcaaccggt aagttcatga tcgccagacc
     5341 acctgcaagc atcataataa tggcaagtac ccaggcaaaa accgggcgat caataaaata
     5401 gttagccatg aaagtcccct taagcctgca acgttattgt ttcgattcgg tgctggcgtt
     5461 ttcctggctg gaggaaattg ctcgtgcttt gatacccgga cgaatgcgtt gcaaaccgga
     5521 aacgatcacc cgatcgccag cctgcaagcc agaggtgaca acccactggt cgccgatggc
     5581 tttgctggct tcaatttcgc gtagctgcac aacatcgtct ttatccagaa tgagcgccgt
     5641 tgctttaccc tgggcgttgt gggtgacgcc ttcctgcggc accagtaata cattctggcg
     5701 gctaccttca tccactaatg ccgtgacgta catgccaggc agcaagtcac catttgggtt
     5761 ggggaaaatc gcccgtaacg tcacggagcc cgtggtttca tccaccgtcg ggtcggagaa
     5821 tttcagcgtg ccggtctggc tgtagcgttt accattttcc agattgagct gtactggcgt
     5881 actgccctga acctgtttga tttgcccact ggcgacctct tctttcatgc gtaagaaatc
     5941 ttgcaccgac tgcgtgagat cgacataaat cgggtccaga cgttgtacgg taaccagcga
     6001 atctgcctga ttagcggtaa cgagtgcgcc gacggtcacc gacgatttcc cgctgacgcc
     6061 cgtaatcggc gaggtgacat tcgcgtattg cagattgatc gtcgcctgtt caacagccgc
     6121 tttggcgacg gtgacattgg cttctgcttc attcaactgg gtgcgcgcgg tgtcgtaatc
     6181 ctgacggcta acgtagttgg tcttcagcaa cgatgcctgg cggttaaagg tgatgcgggc
     6241 attgctggcg gtagagagcg ctttcgccag cgagcctttg gcggagttta gctcggcctg
     6301 taaaggtgca ggatcaatct gatacagcga atcgccctgg ttcactttat cgccttcgat
     6361 aaagttgcgt ttaatgataa taccgcccac ctggggacgt atctcggcaa cttcataagg
     6421 aacggttcta ccgggcaatt cgctcaacac attgaccgaa ccgggggaga gtgtgacgac
     6481 accgacctca ggcgtcatgg cggcggcgtt ttccgccgat ttgtcatcgc aggcggtgag
     6541 catcgcgccg cagaataaca acggtattaa cagctttctt cttctgttca ttttagtccc
     6601 tgaaaattct tgagaaataa cggttcttta aattgcgcgc gaggagagat atgagggatt
     6661 tacagaccag cggcatagtg gggttgtcag ccagtaaggt gggatacagg tatagtgatc
     6721 gacatggtga ggtcaacgac aaaaaaaatg ttgccgttct gccaacagtt cctgccagca
     6781 ttcgggcaac gaagggttct actggtggat acacatacca ggggaataaa gtcgttttag
     6841 cctctccttt agtaatcacc gggggtaatg aaatcagcat ctgcatccct cgtcatgcca
     6901 gccatcaatt tcagttgctt atgtcctgac taaaaataag atgtgatacc cagggtgacg
     6961 atgtcgctca tacgtcttag tttcagcttg tacataatgt tgaatttctg aatctttaat
     7021 gtttgttgcg aaatattgag cgtcgacgtg atattgcttt catttttatt tttcagaatc
     7081 agttgcataa ttttgaactg attatgtgaa aaacataatt gtgaattctt atggggcaag
     7141 tgtttaccat aagtaatctc aagaagattt ttgatacttt ctttgcggct aacaaaataa
     7201 ataggcgctt tagcttttag tttactgatg tgataaggtg ccaaaacaat aattctctcg
     7261 gcatctaatt tctccagaaa tttaatcgct tcttcatcaa ggatatgatt gtgataggta
     7321 tcaatgataa ccttgaaggg tttattgcct acttttttga tctctgacag ggaattgatt
     7381 tttatcattt cgtgattatc tttcaactgc caaaagccct gtaaaagaaa agaatctttc
     7441 gtcatgagaa aaatcataac ttgctcctta gccgttatcg tttcgaacat gtcatccttt
     7501 tcattattta catccttgtc cgaatcgttg ttcaatatag taaacgccta taagaacttt
     7561 gcccttcgtg aactaatacg tctattaaca ctaatcaata cttcagattt atccatcacg
     7621 cactccatat attgatccta agtaagattc ctggttgtta tcagtttgta aacagtaaac
     7681 aaaaagttaa cgtttgcgga ttactgccca agaataagtt atcaataacc agtttgtgat
     7741 ctctgaagaa tattactaaa gttaaaatgt aatccgattt aaatatcgag tctccttgtt
     7801 tcgacttaag ctggcaattg gattgccagc tttcttttaa cgcattgaaa ttacctgagt
     7861 aaataacgca ttcatgtctt caggttttta tatagcgtct ggagacacgg atatttatgc
     7921 aatgtaatca atgccagttg caaaaaaatg aacccggatt acttatttaa ccgcccatag
     7981 catcaacgct tacggaactc tttgttcact ttcccactgc acttttaaaa cattaaacct
     8041 gactacggaa aatatcagcc atgataatgt ttgaaatggc taatttgcca tggagtgaaa
     8101 aaaattagat gaaattcagt aggttgaaat aatcactagc aggtaattat ttcaatgata
     8161 gtgcgcaatt gatctacaac gctgcgtagc ggagagagta ttaatcggat catagtcaca
     8221 tcaagtgact atgatccggg tgacaaccgg gataattatt gctgcttaac gaacaaactg
     8281 gcgaagctga acaggctggc ggcgctaaat atcagttcaa ttcccaccag tgtggaaacc
     8341 agcgttacag acaccatcgg cgttgcacca aggaatatcc aggcaatgac gatatccagc
     8401 acaccaataa cgagctgtag ccagctgcct ttcattgaac gctgacgata ccaactcatc
     8461 aggcgaataa cccctgcaac acagaacaaa ccggcaataa atgccgcaat ggcaaaaatg
     8521 cccagctccg gtgcgcggat gaagaaatag ccgatcaata aataggcgac tgcgacgagg
     8581 aaaccggata ataccggcca gaaattatga ctgcggttgc tgaataaccc gacaataagc
     8641 gcaatgcccg agcagattaa taatgcaccc actactgtgc ttaaaatatc gccagagacg
     8701 aacgggaaac tgatacacag caacccgacg ataaacaaca gcacggcaat aaactggatt
     8761 gctctgcgat gttttttaag catctccaga tcaaacttca aaattgttgc ct
//
LOCUS       DAPHDW010000167         8703 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.170, whole genome shotgun sequence.
ACCESSION   DAPHDW010000167 DAPHDW010000000
VERSION     DAPHDW010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8703
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.170"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(279..542)
                     /gene="rpsT"
                     /locus_tag="RH057_003936"
     CDS             complement(279..542)
                     /gene="rpsT"
                     /locus_tag="RH057_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HDV3130234.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
     gene            645..863
                     /locus_tag="RH057_003937"
     CDS             645..863
                     /locus_tag="RH057_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HDV3130235.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGHFLPVICAMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            871..1812
                     /gene="ribF"
                     /locus_tag="RH057_003938"
     CDS             871..1812
                     /gene="ribF"
                     /locus_tag="RH057_003938"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HDV3130236.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            1855..4671
                     /gene="ileS"
                     /locus_tag="RH057_003939"
     CDS             1855..4671
                     /gene="ileS"
                     /locus_tag="RH057_003939"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HDV3130237.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFAVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWIPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELSAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            4671..5165
                     /gene="lspA"
                     /locus_tag="RH057_003940"
     CDS             4671..5165
                     /gene="lspA"
                     /locus_tag="RH057_003940"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HDV3130238.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVILAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            5253..5702
                     /gene="fkpB"
                     /locus_tag="RH057_003941"
     CDS             5253..5702
                     /gene="fkpB"
                     /locus_tag="RH057_003941"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HDV3130239.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            5704..6654
                     /gene="ispH"
                     /locus_tag="RH057_003942"
                     /gene_synonym="lytB"
     CDS             5704..6654
                     /gene="ispH"
                     /locus_tag="RH057_003942"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HDV3130240.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKSAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            6720..7634
                     /gene="rihC"
                     /locus_tag="RH057_003943"
     CDS             6720..7634
                     /gene="rihC"
                     /locus_tag="RH057_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HDV3130241.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLSQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNFAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVKVALDLDVKGFQQWVAEVLALAS"
     gene            7801..8622
                     /gene="dapB"
                     /locus_tag="RH057_003944"
     CDS             7801..8622
                     /gene="dapB"
                     /locus_tag="RH057_003944"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HDV3130242.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAVKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNSL"
BASE COUNT         1986 a         2162 c         2470 g         2085 t
ORIGIN      
        1 gtcttaagtt taatacctca atgtgtatca caatatccac attctttgta ggggagtctg
       61 gagattgagt agatattctt gttcagaatg tatcagccga tggttctacg attcttaagc
      121 cacgaagagt tcagaaagtg taacggcatg tctgcaaaag ccagcatctt ttgactatct
      181 ggcaaccggt agtgtgttct ctcacgcatc ataaaagcag caggcataaa aaaacccgct
      241 tgcgcgggct ttttcacaaa gcttcagcaa attggcgatt aagccagttt gttgatctgt
      301 gcagtcaggt tagccttatg acgtgcagct ttgtttttgt ggatcagacc tttagcagcc
      361 tgacggtcca cgatcggttg catttcgtta aatgctttct gtgcagcagc tttgtcgcca
      421 gcttcgatag ctgcgtatac tttcttgatg aaagtacgca tcatagagcg acggcttgcg
      481 ttgtgcttac gagccttttc agactgaatg gcgcgcttct tagctgattt gatattagcc
      541 aaggtccaac tcccaaatgt gttctatatg gacaattcaa aggccgagga atatgccctt
      601 ttagccttct tttgtcaatg gatttgtgca aataagcgcc gttaatgtgc cggcactcgt
      661 tacgtagtga tggcgcagga ttctaccagc ttgcggggtg tgaatacagc ttttccgcga
      721 taaaaattgc agcaggcggt cactttcttc ccgtgatttg cgccatggca atgaaaagcc
      781 acttctttct gatttcggta ctcaatcgcc ggttaacctt gaccgctgta caaggtatac
      841 tcggacgatt ttcactgttt tgagccagac atgaagctga tacgcggcat acataatctc
      901 agccaggccc cgcaagaagg gtgtgtgctg actattggta atttcgacgg cgtgcatcgc
      961 ggtcatcgcg cgctgttaca gggcttgcag gaagaagggc gcaagcgcaa cttaccggtg
     1021 atggtgatgc tttttgaacc tcaaccactg gaactgtttg ctaccgataa agccccggca
     1081 cggctgaccc ggctgcggga aaaactgcgt taccttgcag agtgtggcgt tgattacgtg
     1141 ctgtgcgtgc gtttcgacag gcgtttcgcg gcgttaaccg cgcaaaattt catcagcgat
     1201 cttctggtga agcatttgcg cgtaaaattt cttgccgtag gtgatgattt ccgctttggc
     1261 gctggtcgtg aaggcgattt cttgttatta cagaaagctg gcatggaata cggcttcgat
     1321 atcaccagta cgcaaacttt ttgcgaaggt ggcgtgcgta tcagcagcac cgctgtgcgt
     1381 caggcgctgg cggatgacaa tctggctctg gcagaaagtt tactggggca cccgtttgcc
     1441 atctccgggc gtgtagtcca cggtgatgaa ttagggcgca ctataggttt cccgacggcg
     1501 aatgtaccgc tacgccgtca ggtttccccg gtgaaagggg tttatgcggt agaagtgctg
     1561 ggcctcggtg aaaagccgtt acccggcgtg gcaaacattg gaacacgccc aacggttgcc
     1621 ggtattcgcc agcagctgga agtgcatttg ttagatgttg caatggacct ttacggtcgc
     1681 catatacaag tagtgctgcg taaaaaaata cgcaatgagc agcgatttgc gtcgctggac
     1741 gaactgaaag cgcagattgc gcgtgatgaa ttaaccgccc gcgaattttt tgggctaaca
     1801 aaaccggctt aagcctgtta tgtaatcaaa ccgaaacacg gaaccgagaa tctgatgagt
     1861 gactataaat caaccctgaa tttgccggaa acagggttcc cgatgcgtgg cgatctcgcc
     1921 aagcgcgaac cgggaatgct ggcgcgttgg actgatgatg atctgtacgg catcatccgt
     1981 gcggctaaaa aaggcaaaaa aaccttcatt ctgcatgatg gccctcctta tgcgaatggc
     2041 agcattcata ttggtcactc ggttaacaag attctgaaag acattatcgt gaagtccaaa
     2101 gggctttccg gttatgactc gccgtatgtg cctggctggg actgccacgg tctgccgatc
     2161 gagctgaaag tagagcaaga atacggtaag ccgggtgaga aattcaccgc cgccgagttc
     2221 cgcgccaagt gccgcgaata cgcggcgacc caggttgacg gtcaacgcaa agactttatc
     2281 cgtctgggcg tgctgggcga ctggtcgcac ccgtacctga ccatggactt caaaactgaa
     2341 gccaacatca tccgcgcgct gggcaaaatc atcggcaacg gtcacctgca caaaggcgcg
     2401 aagccagttc actggtgcgt tgactgccgt tctgcgctgg cggaagcgga agttgagtat
     2461 tacgacaaaa cttctccgtc catcgacgtt gcttttcagg cggtcgatca ggatgcactg
     2521 aaagcaaaat ttgccgtaag caacgttaac ggcccaatct cgctggtgat ctggaccact
     2581 acgccgtgga ctctgcctgc gaaccgcgca atctctattg cacctgattt cgactatgcg
     2641 ctggtgcaga tcgacggtca ggccgtgatt ctggcgaaag atctggttga aagcgtaatg
     2701 cagcgtatcg gcgtgaccga ttacaccatt ctcggcacgg taaaaggtgc ggagcttgag
     2761 ttgctgcgct ttacccatcc gtttatgggc ttcgacgttc cggcaatcct cggcgatcac
     2821 gttaccctgg atgccggtac cggtgccgtt cacaccgcgc ctggccacgg cccggacgac
     2881 tatgtgatcg gtcagaaata cggcctggaa accgctaacc cggttggccc ggacggcact
     2941 tatctgccgg gcacttatcc gacgctggat ggcgtgaacg tcttcaaagc gaacgacatc
     3001 gtcgttgcgc tgctgcagga aaaaggcgcg ctgctgcacg ttgagaaaat gcagcacagc
     3061 tatccgtgct gctggcgtca caaaacgccg atcatcttcc gcgcgacgcc gcagtggttc
     3121 gtcagcatgg atcagaaagg tctgcgtgcg cagtcactga aagagatcaa aggcgtgcag
     3181 tggatcccgg actggggcca ggcgcgtatc gagtcgatgg ttgctaaccg tcctgactgg
     3241 tgtatctccc gtcagcgcac ctggggcgta ccgatgtcac tgttcgtgca caaagacacg
     3301 gaagagctgc atccgcgtac tctcgaacta atggaagaag tggcaaaacg cgttgaagtt
     3361 gacggcatcc aggcgtggtg ggatcttgat gcgaaagaga tcctcggcga tgaagctgat
     3421 cagtacgtga aagtgccgga cacattggat gtatggtttg actccggatc tacccactct
     3481 tctgttgttg acgtgcgtcc ggaatttgcc ggtcacgcag cggacatgta tctggaaggt
     3541 tctgaccagc accgtggttg gttcatgtct tccctgatga tctccaccgc gatgaaaggc
     3601 aaagcgccgt atcgtcaggt actgacccac ggctttaccg tggatggtca gggtcgcaag
     3661 atgtctaaat ccatcggcaa taccgtttcg ccgcaggatg tgatgaacaa actgggcgcg
     3721 gatattctgc gtctgtgggt ggcatcaacc gactacaccg gtgaaatggc cgtttctgac
     3781 gagatcctga aacgtgctgc cgatagctat cgtcgtatcc gtaacaccgc gcgcttcctg
     3841 ctggcaaacc tgaacggttt tgatccagca aaagatatgg tgaaaccgga agagatggtg
     3901 gtactggatc gctgggccgt aggttgtgcg aaagcggcac aggaagacat cctcaaggcg
     3961 tacgaagcat acgatttcca cgaagtggta cagcgtctga tgcgcttctg ctccgttgag
     4021 atgggttcct tctacctcga catcatcaaa gaccgtcagt acaccgccaa agcggacagt
     4081 gtggcgcgtc gtagctgcca gactgcgctg tatcacatcg cagaagcgct ggtgcgctgg
     4141 atggcaccaa tcctctcctt caccgctgat gaagtgtggg gctacctgcc gggcgaacgt
     4201 gaaaaatacg tcttcaccgg tgagtggtac gaaggcctgt ttggcctggc agacagtgaa
     4261 gcgatgaacg atgcgttctg ggacgagctg ttgaaagtgc gtggcgaagt gaacaaagtc
     4321 attgagcaag cgcgtgccga caagaaagtg ggtggctcgc tggaagcggc agtaaccttg
     4381 tatgcagaac cggaactgtc ggcgaaactg accgcgctgg gcgatgaatt acgatttgtc
     4441 ctgttgacct ccggcgctac cgttgcagac tataacgacg cacctgctga tgctcagcag
     4501 agcgaagtac tcaaagggct gaaagtcgcg ttgagtaaag ccgaaggtga gaagtgccca
     4561 cgctgctggc actacaccca ggatgtcggc aaggtggcgg aacacgcaga aatctgcggc
     4621 cgctgtgtca gcaacgtcgc cggtgacggt gaaaaacgta agtttgcctg atgagtcaat
     4681 cgatctgttc aacagggcta cgctggctgt ggctggtggt agtcgtgctg attatcgatc
     4741 tgggcagcaa atacctgatc ctccagaact ttgctctggg ggatacggtc ccgctgttcc
     4801 cgtcgcttaa tctgcattat gcgcgtaact atggcgcggc gtttagtttc cttgccgata
     4861 gcggcggctg gcagcgttgg ttctttgccg gtattgcgat tggtattagc gtgatcctgg
     4921 cagtgatgat gtatcgctcg aaggccacgc agaagctaaa caatatcgct tatgcgctga
     4981 ttattggcgg cgcgctgggc aacctgttcg accgcctgtg gcacggcttc gttgtcgata
     5041 tgatcgactt ctacgtcggc gactggcact tcgccacctt caaccttgcc gatactgcca
     5101 tttgtgtcgg tgcggcactg attgtgctgg aaggtttttt gccttctaaa gcgaaaaaac
     5161 aataataaac cctgccggat aaggcctaca gattgctgcg aaatcgtagg ccgcatccgg
     5221 caaaaatcct caaaatataa gagcaaacct gcatgtctga atctgtacag agcaatagcg
     5281 ccgtcctggt gcacttcacg ctaaaactcg acgatggcac caccgccgag tctacccgca
     5341 acaacggtaa accggcgctg ttccgcctgg gtgatgcttc tctttctgaa gggctggagc
     5401 aacacctgtt ggggctgaaa gtgggcgata aaaccacctt ctcgttggag ccagatgcgg
     5461 cgtttggcgt gccgtcaccg gacctgattc agtacttctc ccgccgtgaa tttatggatg
     5521 caggcgagcc agaaattggc gcaatcatgc tttttaccgc aatggatggc agtgagatgc
     5581 ctggcgtgat ccgcgaaatt aacggcgact ccattaccgt tgatttcaac catccgctgg
     5641 ccgggcagac cgttcatttt gatattgaag tgctggaaat cgatccggca ctggaggcgt
     5701 aacatgcaga tcctgttggc caacccgcgt ggtttttgtg ccggggtaga ccgcgctatc
     5761 agcattgttg aaaacgcgct ggccatttac ggcgcaccga tatatgtccg tcacgaagtg
     5821 gtacataacc gctatgtggt cgatagcttg cgtgagcgtg gggctatctt tattgagcag
     5881 attagcgaag taccggacgg cgcgatcctg attttctccg cacacggtgt ttctcaggcg
     5941 gtacgtaacg aagcaaaaag tcgcgatttg acggtatttg acgcgacctg tccgctggtg
     6001 accaaagtgc atatggaagt cgcccgcgct agtcgccgtg gcgaagaatc tattctcatc
     6061 ggtcacgccg ggcacccgga agtggaaggc acgatgggtc agtacagcaa cccggaaggg
     6121 ggaatgtatc tggttgaatc accagacgat gtgtggaaac tgacggtcaa aaacgaagag
     6181 aaactctcct ttatgaccca gaccacgctg tcggtggatg acacgtctga tgtgatcgac
     6241 gcgctgcgta aacgcttccc gaaaattgtc ggtccgcgca aagatgacat ctgctacgcc
     6301 acgactaacc gacaggaagc ggtacgtgcc ctggccgaac aggcggaagt tgtgctggtg
     6361 gtcggttcga aaaactcctc taactccaac cgtctggcgg agctggccca gcgtatgggc
     6421 aaaagcgcgt ttttgattga tgatgcgaaa gatatccagg aagagtgggt gaaagaggtt
     6481 aaatgcgtcg gcgtgactgc gggcgcatcg gctccggata ttctggtgca gaatgtggtg
     6541 gcacgtttgc aacagctggg cggtggtgaa gccattccgc tggaaggacg tgaagaaaac
     6601 attgttttcg aagtgccgaa agagctgcgt gtcgatattc gtgaagtcga ttaagtcatt
     6661 agcagcctaa gttatgcgaa aatgccggtc ttgttaccgg cattttttat ggagaaaaca
     6721 tgcgtttacc tatcttcctc gatactgacc ccggcattga cgatgccgtc gccattgccg
     6781 ccgcgatttt tgcacccgaa ctcgacctgc aactgatgac caccgtcgcg ggtaatgtct
     6841 cggttgagaa aactacccgc aatgccctgc aactgctgca tttctggaat gcggagattc
     6901 cgctcgccca aggggccgct gtgccgctgg tacgcgcacc gcgtgatgcg gcatctgtgc
     6961 acggcgaatc gggaatggct ggctacgact ttgttgagca caaccgaaag ccgctcggga
     7021 taccggcgtt tctggcgatt cgggatgccc tgatgcgtgc accagagcct gttaccctgg
     7081 tggccattgg cccgttaacc aatattgcgc tgttactttc acaatgcccg gaatgcaagc
     7141 cgtatattcg ccgtctggtg atcatgggtg gttctgccgg acgcggcaac tgtacgccaa
     7201 acgccgagtt taatttcgct gccgatccag aagcggctgc ctgtgtcttc cgtagcggaa
     7261 tcgaaatcgt catgtgcggt ctggatgtca ccaatcaggc aatattaacc cctgactatc
     7321 tcgctacttt gccggagttg aatcgcaccg gaaaaatgct tcatgcactg tttagccact
     7381 accgtagcgg cagtatgcaa agcggtctgc gaatgcacga tctctgcgcc atcgcctggc
     7441 tggtgcgccc ggacttattc accctcaagc cgtgttttgt cgcggtagaa actcaaggcg
     7501 aatttacctc aggcacgacg gtggttgata tcgacggttg cctgggcaaa ccagccaatg
     7561 taaaggtggc attggatctg gatgtgaaag gcttccagca gtgggtggct gaggtgctgg
     7621 ctctggcgtc gtaacctgtc acatgttatc ggcatgcagt cattcatcga ctcatgcctt
     7681 tcactgatat ccccccctgt ttatcattaa tttctaatta tcagcgtttt tggctggcgg
     7741 cgtagcgatg cgctggttac tctgaaaacg gtctatgcaa attaacaaaa gagaatagct
     7801 atgcatgatg caaacatccg cgttgccatc gcgggagccg gggggcgtat gggccgccag
     7861 ttgattcagg cggcgctggc attagaaggc gtgcagctgg gcgcggcgct ggagcgcgaa
     7921 ggatcttctt tactgggcag cgacgccggt gagctggctg gtgcagggaa aacaggcgtt
     7981 accgtgcaaa gcagcctcga tgcggtaaaa gatgattttg atgtgtttat cgattttacc
     8041 cgtccggaag gtacgctgaa ccatctcgct ttttgtcgcc agcatggcaa agggatggtg
     8101 atcggcacta cggggtttga cgaagccggt aaacaggcga ttcgtgacgc cgccgccgat
     8161 attgcgattg tctttgccgc caattttagc gttggcgtta acgtcatgct taagctgctg
     8221 gagaaagcag ccaaagtgat gggtgactac accgatatcg aaattattga agcacatcat
     8281 agacataaag ttgatgcgcc gtcaggcacc gcactggcaa tgggagaggc gatcgcccac
     8341 gcccttgata aagatctgaa agattgcgcg gtctacagtc gtgaaggcca caccggtgaa
     8401 cgtgtgcctg gcaccattgg ttttgccacc gtgcgtgcag gtgacatcgt tggcgaacat
     8461 accgcgatgt ttgccgatat tggcgagcgt ctggagatca cccataaggc gtccagccgc
     8521 atgacatttg ctaacggcgc ggtaagatcg gctttgtggt tgagtggtaa ggaaagtggt
     8581 ctttttgata tgcgagatgt gcttgatctc aatagtttgt aaccacaaaa tatttgttat
     8641 ggtgcaaaaa taacacattt aatttattga ttgtaaaggg ctttaatttt tggccctttt
     8701 att
//
LOCUS       DAPHDW010000168         8655 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.171, whole genome shotgun sequence.
ACCESSION   DAPHDW010000168 DAPHDW010000000
VERSION     DAPHDW010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8655
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.171"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            210..1313
                     /gene="ompC"
                     /locus_tag="RH057_003945"
     CDS             210..1313
                     /gene="ompC"
                     /locus_tag="RH057_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV3130243.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDNKDVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRNTDFFGL
                     VDGLNFAVQYQGKNGSVSGEGMTNNGRDALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDAQNTAAYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGSLGWANKAQNF
                     EAVAQYQFDFGLRPSVAYLQSKGKNLGTIAGRNYDDEDILKYVDVGATYYFNKNMSTY
                     VDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            1425..2480
                     /gene="apbE"
                     /locus_tag="RH057_003946"
     CDS             1425..2480
                     /gene="apbE"
                     /locus_tag="RH057_003946"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HDV3130244.1"
                     /translation="MEISFTRVALLAAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MAEIVTTSLRIGAKTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYIDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLISEKN"
     gene            2554..3618
                     /gene="ada"
                     /locus_tag="RH057_003947"
     CDS             2554..3618
                     /gene="ada"
                     /locus_tag="RH057_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HDV3130245.1"
                     /translation="MKKATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANAQQHRLDKITHACRLLEQETPVTL
                     EALADHVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADESLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            3618..4268
                     /gene="alkB"
                     /locus_tag="RH057_003948"
     CDS             3618..4268
                     /gene="alkB"
                     /locus_tag="RH057_003948"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HDV3130246.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVTPGGYTMSVAMTNCGHLGWTSHRQGYLYSPIDPQTNKPWPAMPQSFHNLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            4344..5987
                     /gene="yojI"
                     /locus_tag="RH057_003949"
     CDS             4344..5987
                     /gene="yojI"
                     /locus_tag="RH057_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HDV3130247.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELVQGIILTIGSAAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFSYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGKPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAADQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            6205..7851
                     /gene="mqo"
                     /locus_tag="RH057_003950"
     CDS             6205..7851
                     /gene="mqo"
                     /locus_tag="RH057_003950"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HDV3130248.1"
                     /translation="MKKVTAMLFSMAVGLNAVSMAAKAKASEEQETDVLLIGGGIMSA
                     TLGTYLRELEPEWSMTMVERLEGVAQESSNGWNNAGTGHSALMELNYTPQNADGSISI
                     EKAVAINEAFQISRQFWAHQVERGVLRTPRSFINTVPHMSFVWGEDNVNFLRARYAAL
                     QQSSLFRGMRYSEDHAQIKEWAPLVMEGRDPQQKVAATRTEIGTDVNYGEITRQLIAS
                     LQKKSNFSLQLSSEVRALKRNDDNTWTVTVADLKNGTAQNIRAKFVFIGAGGAALKLL
                     QESGIPEAKDYAGFPVGGQFLVSENPDVVNHHLAKVYGKASVGAPPMSVPHIDTRVLD
                     GKRVVLFGPFATFSTKFLKNGSLWDLMSSTTTSNVMPMMHVGLDNFDLVKYLVSQVML
                     SEEDRFEALKEYYPQAKKEDWRLWQAGQRVQIIKRDAEKGGVLRLGTEVVSDQQGTIA
                     ALLGASPGASTAAPIMLDLLEKVFGDRVSSPQWQATLKAIVPSYGRKLNCDVAATERE
                     LQYTSEVLGLKYDKPQAADSTPKPQLKPQPVQKEVADIAL"
     gene            complement(8000..8488)
                     /gene="eco"
                     /locus_tag="RH057_003951"
     CDS             complement(8000..8488)
                     /gene="eco"
                     /locus_tag="RH057_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HDV3130249.1"
                     /translation="MKTILPAVLFAAFATTSAWAAESVQPLEKIAPYPQAEKGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAV
                     VR"
BASE COUNT         2078 a         2165 c         2331 g         2081 t
ORIGIN      
        1 tccgcaataa tcttaaaaag ttcccttgca tttacatttt gaaacatcta tagcgataaa
       61 tgaaacatct taaaagtttt agtatcatat tcgtgttgga ttattctgca tttttgggga
      121 gaatggactt gccgactgat taatgagggt taatcagtat gcagtggcat aaaaaagcaa
      181 ataaaggcat ataacagagg gttaataaca tgaaagttaa agtactgtcc ctcctggtcc
      241 cagctctgct ggtagcaggc gcagcaaacg ctgctgaagt ttacaacaaa gacggcaaca
      301 aattagatct gtacggtaaa gtagacggcc tgcactattt ctctgacaac aaagatgtag
      361 atggcgacca gacctacatg cgtcttggct tcaaaggtga aactcaggtt actgaccagc
      421 tgaccggtta cggccagtgg gaatatcaga tccagggcaa cagcgctgaa aacgaaaaca
      481 actcctggac ccgtgtggca ttcgcaggtc tgaaattcca ggatgtgggt tctttcgact
      541 acggtcgtaa ctacggcgtt gtttacgacg taacttcctg gaccgacgta ctgccagaat
      601 tcggtggcga cacctacggt tctgacaact tcatgcagca gcgtggtaac ggctttgcga
      661 cctaccgtaa cactgacttc ttcggtctgg ttgacggcct gaactttgct gttcagtacc
      721 agggcaaaaa cggtagcgta agcggcgaag gcatgaccaa caatggtcgc gatgctctgc
      781 gtcagaacgg cgacggcgtt ggcggttcta tcacttatga ttacgaaggc ttcggtatcg
      841 gtgctgcagt ttccagctcc aaacgtactg atgctcagaa caccgctgct tacatcggta
      901 acggcgaccg tgctgaaacc tacaccggtg gtctgaaata cgacgctaac aacatctacc
      961 tggctgctca gtacacccag acctataacg caactcgcgt aggttccctg ggttgggcga
     1021 acaaagcaca gaactttgaa gctgttgctc agtaccagtt cgacttcggt ctgcgtccgt
     1081 ctgtagcata cctgcagtct aaaggtaaaa acctgggtac catcgctggt cgtaactacg
     1141 acgacgaaga tatcctgaaa tatgttgatg ttggcgcgac ctactacttc aacaaaaaca
     1201 tgtccaccta cgttgactac aaaatcaacc tgctggacga caaccagttc actcgtgacg
     1261 ctggcatcaa cactgataac atcgtagctc tgggtctggt ttaccagttc taatctcgat
     1321 tgatatcgaa caaagggcct gcgggccctt ttttcattgt tttcagcgta caaactcatt
     1381 tttttggtgt actcttgcga ccgttcgcat gaggataatc acgtatggaa ataagcttta
     1441 cccgcgtggc actgctggct gccgcgctct tctttgttgg ttgcgatcaa aaaccacaac
     1501 ccgccaaaac ccacgctact gaagttaccg ttcttgaagg caaaaccatg ggtaccttct
     1561 ggcgtgccag catcccgggc attgatgcca aacgcagtgc cgaacttaaa gaaaagattc
     1621 agacccagct ggacgccgac gatcagctgc tttcgaccta taaaaaagat tccgcgctga
     1681 tgcgctttaa cgactcgcaa agtttgtcgc cgtggccagt aagtgaagcg atggccgaaa
     1741 tcgtcactac ctcgctgcgc attggcgcga agaccgatgg tgcgatggat attaccgtcg
     1801 ggccgctggt gaatctgtgg ggctttggcc cggaacaaca gccggttcaa attccgagcc
     1861 aggaacagat cgatgcgatg aaagccaaaa ccggcttaca gcacctgacg gtcatcaatc
     1921 agtcgcatca gcaatatctg caaaaagacc tgccggattt atatatcgat ctctccaccg
     1981 tcggtgaagg ttatgcggcg gatcacctgg cacgcttgat ggagcaggaa gggatttccc
     2041 gctatctggt gtcggtgggc ggcgcgctga acagccgtgg tatgaacggt gaaggccagc
     2101 cgtggcgggt agcgattcaa aaaccaaccg ataaagaaaa cgcggttcag gctgtggtgg
     2161 atatcaacgg tcatgggatt agcacctctg gcagctaccg taactattac gaactggacg
     2221 gcaaacgtct ttcccatgtt atcgatccgc aaaccgggcg tcccatcgaa cacaatctgg
     2281 tatccgtgac ggtgattgct ccgacggcgc tggaagccga tgcctgggat actggcttga
     2341 tggtactcgg gccggagaaa gccaaagagg ttgttcgccg ggaagggctg gcggtctata
     2401 tgatcaccaa agaaggcgat agctttaaaa cctggatgtc gccgcagttt aaaagcttcc
     2461 ttatcagcga aaaaaattaa agcgcaagat tgttggtttt tgcgtgatgg tgaccgggca
     2521 gactaaaggc tatccttaac cagggagctg attatgaaaa aagccacatg cttaactgac
     2581 gatcaacgct ggcaatctgt cttagcccgc gacccgaatg ccgacggcga attcgttttc
     2641 gccgtgcgta ccacaggcat cttttgccgt ccgtcttgcc gcgccagaca tgctttgcgg
     2701 gaaaacgtct ccttctacgc aaatgccagc gaggcactcg ccgctggctt tcgcccctgc
     2761 aaacgttgtc agccagacaa agccaatgcc cagcaacatc ggttggataa aatcacccac
     2821 gcctgtcgac tgctggaaca ggaaacgcct gtaacgctgg aagccttagc cgaccatgtg
     2881 gcgatgagtc cgttccatct gcatcggttg tttaaagcga ctaccggaat gacgcctaaa
     2941 gcctggcaac aggcctggcg cgctcgccgt ttgcgcgaat cgctggcgaa aggggagagc
     3001 gtgacgacgt ctattcttaa cgccggattc cccgacagca gcagttacta tcgcaaagct
     3061 gacgaatcgc tgggcatgac ggctaaacaa ttccgtcacg gtggcgaaaa tctggcggtg
     3121 cgttacgcgc tggctgattg tgagctgggt cgttgcctgg tggcagaaag cgagcggggg
     3181 atttgcgcga tattgctggg cgatgatgac gcgacgctaa tcagcgagtt gcagcagatg
     3241 tttcccgctg ccgacaacgc gcctgccgat ctgacgtttc agcaacatgt gcgtgaagtg
     3301 atcgccagcc tcaatcaacg cgatacgccg ctgacgttac cgctggacat tcgcggcact
     3361 gcttttcagc aacaagtctg gcaggcactg cgcacgatac cttgcggtga aaccgtcagt
     3421 tatcagcaac tggctaacgc catcggcaaa ccgaaagcgg tacgggccgt tgccagcgcc
     3481 tgtgccgcca acaagctggc tatcgtaata ccctgtcatc gggtggtccg tggtgatggc
     3541 acactttccg gttaccgctg gggcgtgtcg cgtaaagcgc aactgctgcg ccgcgaagct
     3601 gaaaatgagg agaggtaatg ttggatctgt ttgccgatgc tgaaccgtgg caagagccac
     3661 tggcggctgg tgcggtaatt ttacggcgtt ttgcttttaa cgctgcggag caactgatcc
     3721 gcgatattaa tgacgttgcc agccagtcgc cgtttcgcca gatggtcacc cccgggggat
     3781 ataccatgtc ggtggcgatg accaactgtg ggcatctggg ctggacgagc catcggcaag
     3841 gttatctcta ttcgcccatt gatccgcaaa caaataaacc gtggcccgcc atgccacaga
     3901 gttttcataa tttatgtcaa cgtgcggcta cggcggcggg ctatccagat ttccagccag
     3961 atgcttgtct tatcaaccgc tacgctcctg gcgcgaaact gtcgctgcat caggataaag
     4021 acgaaccgga tctgcgcgcg ccaattgttt ctgtttctct gggcttaccc gcgatttttc
     4081 aatttggtgg cctgaaacgt aatgatccgc tcaaacgttt gttgttggaa catggcgatg
     4141 tggtggtatg gggcggtgaa tcgcggctgt tttatcacgg tattcaaccg ttgaaagcgg
     4201 ggtttcatcc actcaccacc gactgccgct acaacctgac attccgtcag gcaggtaaaa
     4261 aagaataaaa ataagaatta ttattgctgt gcgcgcgaag atgtttaaac tgcgggctgt
     4321 aattcattgt ccgggttttc tgcatggaac ttcttgtact tgtctggcgg cagtatcgct
     4381 ggccatttat cagtgtgatg gcgctaagcc tcgccagtgc ggcattaggc attggcttaa
     4441 ttgcttttat caatcagcgc cttatcgaaa cggcggatac cagtctgctg gtattgccgg
     4501 agtttctggg attattgctg ctgttgatgg cagtcactct cggatcgcaa ctggcgctca
     4561 ccactttggg gcatcacttc gtttaccgac tgcgtagtga atttatcaag cggattctgg
     4621 atacccacgt cgagcgcatt gaacaactcg gtagcgcctc gttgctggcg ggattaacca
     4681 gcgatgtgcg caatatcacc attgcttttg tgcgtcttcc ggaactggtg caggggatca
     4741 ttctcactat cggttccgcg gcgtatctgt ggatgctgtc gggcaaaatg ttgctggtaa
     4801 cggctatctg gatggcgatc accatctggg gtggttttgt actggtggcg cgggtgtaca
     4861 aacatatggc gaccctgcgt gaaaccgaag acaagctgta cacggatttt caaaccgtac
     4921 tcgaagggcg caaagagctg actctgaacc gggaacgcgc cgagtatgtg tttaacaacc
     4981 tctacatccc cgatgcgcaa gagtatcgcc atcatattat tcgcgcagac accttccatc
     5041 ttagcgccgt gaactggtca aacatcatga tgctgggcgc tatcggcctg gtgttctgga
     5101 tggcgaacag tctcggttgg gctgatacca acgttgctgc gacctattcg ttgacgctgt
     5161 tattcctgcg tacgccgctg ctttcggcgg ttggcgcatt gccgacgctg ctgacggcgc
     5221 aggtggcgtt taacaagctg aacaaattcg cgctcgcgcc tttcaaagct gagtttccgc
     5281 gcccgcaggc ttttcccaac tggcaaacgc tggagctgcg taacgtgacg tttagctatc
     5341 aggataacgc gttttccgtt gggccgatta acctcaccat taaacgtggc gagctgctgt
     5401 ttctgattgg tggcaacggt agtggaaaat cgacgctggc gatgttgctg acgggcttgt
     5461 accagccaca aagcggcgaa atcttgctgg atggcaaacc tgtcagtggc gaacaaccgg
     5521 aagattatcg caaactgttt tcggcagtgt ttaccgatgt ctggctgttt gatcaactgc
     5581 tggggccgga gggtaaaccc gctaacccgc aactggttga gaagtggctg gcgcagctga
     5641 aaatggctca taagcttgag ttaagcaacg ggcgtattgt taacctgaag ttatcaaaag
     5701 ggcagaaaaa acgcgtggcg ctgttgctgg cgctggcaga agaacgcgat attatcctgc
     5761 tggatgaatg ggcggcggat caggatccac acttccgtcg tgagttttat caggtgttgc
     5821 tgccgctgat gcaggagatg ggtaaaacta ttttcgctat cagtcatgat gatcattact
     5881 ttatccacgc cgaccgcctg ctggaaatgc gcaatgggca acttagcgag ctgacgggcg
     5941 aagagcgcga tgccgcttcg cgtgatgccg ttgcccggac ggcataatat tacgacgctg
     6001 acctggcgga ctgctgccgt caggtcaata tgctaaaaat cccttcattt ctaataaccc
     6061 tataattaat caacgaaatt ataatgtttc taaaattaga atataattta taaacattat
     6121 ttaaatgttg ttacttaagt gttaccgttg atgccgcgca aatctcacct gcaataaagc
     6181 gactaaaagt aaggcattaa caagatgaaa aaagtgactg ccatgctctt ctcgatggcc
     6241 gtggggctta atgccgtttc gatggcggca aaagcgaaag cgtccgagga gcaggaaact
     6301 gatgtactgt tgattggcgg cggcattatg agcgccacgt tggggaccta tttacgcgag
     6361 ctggagcctg aatggtcgat gaccatggtg gagcgcctgg agggtgtcgc gcaggagagt
     6421 tcgaacggct ggaataacgc cggaaccggg cattctgcac tgatggaact gaactacacc
     6481 ccgcaaaacg ccgatggcag catcagtatt gaaaaagcag tcgccattaa cgaagcattt
     6541 cagatttccc gccagttctg ggcgcaccag gttgagcgcg gcgtgctgcg tactccgcgt
     6601 tcatttatca ataccgttcc gcatatgagc tttgtctggg gcgaggataa cgttaatttc
     6661 ctgcgcgccc gttacgccgc gttgcaacaa agctcgctgt ttcgcggtat gcgttactct
     6721 gaagatcacg cgcagatcaa agagtgggca ccgttagtga tggaagggcg cgatccgcaa
     6781 cagaaagtgg cagccacgcg tacggaaatt ggtaccgatg tgaactacgg cgagatcacc
     6841 cgccagttaa ttgcttcctt gcagaagaaa tctaacttct cgctgcaact cagcagcgaa
     6901 gtccgcgccc taaagcgtaa tgacgataac acctggaccg ttaccgttgc cgatctgaaa
     6961 aacggcactg cacagaacat ccgcgccaaa tttgtcttta tcggcgcggg cggtgcggcg
     7021 ctgaagctat tacaggaatc ggggattccg gaagcgaaag actacgccgg tttcccggtg
     7081 ggcggacagt tccttgtttc ggaaaacccg gacgtggtta atcaccatct ggcgaaggtt
     7141 tacggtaaag catccgttgg cgcaccaccg atgtcggttc cgcatatcga tacccgcgtt
     7201 ctggacggta aacgcgtagt gctgtttggg ccatttgcca ccttctcaac caaattcctc
     7261 aaaaacggtt cattgtggga tctgatgagt tccaccacca cctctaacgt gatgccgatg
     7321 atgcacgtcg ggctggataa tttcgatctg gtgaaatatc tggtgagtca ggtgatgttg
     7381 agtgaagagg atcgttttga agcgttgaaa gagtactatc cgcaggcaaa aaaagaggac
     7441 tggcgtttgt ggcaagcggg gcagcgcgtg cagattatca agcgtgatgc cgagaaaggt
     7501 ggcgtactgc gtctgggtac tgaagtggtc agtgaccagc aagggaccat tgccgcgctg
     7561 ctgggggcat cgccaggggc gtcaaccgcc gcgccgatta tgttggatct gctggaaaaa
     7621 gtatttggcg atcgcgtttc cagcccgcaa tggcaggcta cgttgaaagc gatcgttccg
     7681 tcttatggac gcaagctgaa ctgtgatgta gcggcaacag aacgcgagtt gcagtacacc
     7741 agcgaagtgc tggggctgaa atacgacaag ccgcaggcag cagatagtac gccaaaaccg
     7801 cagttgaaac cgcaacccgt tcaaaaagaa gtggcggata ttgcgttgta atgatgtgcc
     7861 acatccggca tggtatgccg gatgtggcgt atgctgataa gacgcgccag cgtcgcatca
     7921 ggcaccggct cgggcgttag atgtcggatg cggcgtgacc gccttattcg gcctacgggg
     7981 tgccgcactt cacagcattt tagcgaacta ccgcgttgtc aattttctct tccgccttcc
     8041 agacgcggta cttcacatct acattgtctg gcgtatacac cacgatcggc agcttgctgt
     8101 tgtaacgcag cattccagca tcgcccagat acgcggtgac aaatttcttc tctttcttgc
     8161 catccgggca ggccatcatc gtggaaaccg gcgagctaac tttatcaaag acataataat
     8221 catagcccca gccttccagc gttttgcttt ccagcttccc gccgagacga tgcaaattgc
     8281 aatcgacttc cagcgtctga ccgattaaca gttctacttt cagggtagat tcatcttctt
     8341 gcggggttaa ctgaatcacc tgacgcttca tccctttttc agcttgtgga taaggcgcga
     8401 ttttttccag tggctggacg ctttctgccg cccaggcgga agtggtagcg aaagcggcaa
     8461 acaatactgc aggtagaatg gtcttcattt ttttgctttc cttcttcatt tataggtttt
     8521 caccgatgat gtcgctgaag gttaacatat tcatcagatt tattaatttg ctttatgaat
     8581 aacttaaaga acattcagac aattttacct gctcttttag tgttaaattc tggtgaggga
     8641 gcaaaccagc agaaa
//
LOCUS       DAPHDW010000169         8602 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.172, whole genome shotgun sequence.
ACCESSION   DAPHDW010000169 DAPHDW010000000
VERSION     DAPHDW010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8602
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.172"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            43..747
                     /locus_tag="RH057_003952"
     CDS             43..747
                     /locus_tag="RH057_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS26 family transposase"
                     /protein_id="HDV3130250.1"
                     /translation="MNPFKGRHFQRDIILWAVRWYCKYGISYRELQEMLAERGVNVDH
                     STIYRWVQRYAPEMEKRLRWYWRNPSDLCPWHMDETYVKVNGRWAYLYRAVDSRGRTV
                     DFYLSSRRNSKAAYRFLGKILNNVKKWQIPRFINTDKAPAYGRALALLKREGRCPSDV
                     EHRQIKYRNNVIECDHGKLKRIINATLGFKSMKTAYATIKGIEVMRALRKGQASAFYY
                     GDPLGEMRLVSRVFEM"
     gene            complement(800..907)
                     /locus_tag="RH057_003953"
                     /pseudo
     CDS             complement(800..907)
                     /locus_tag="RH057_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            1033..3918
                     /locus_tag="RH057_003954"
     CDS             1033..3918
                     /locus_tag="RH057_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDV3130251.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRR
                     AHNRFGFALQLCVLRYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHE
                     HLAELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATS
                     TIERLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGS
                     NSSSANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLA
                     ILAVCVSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIG
                     GALVDAQDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRY
                     APRMLRLLDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDAR
                     LWEIAVLFHLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLAD
                     RQARLDMRLRELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPT
                     RITDLLLEVDAATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHT
                     FWELIRIGRWHVEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEA
                     MNLVNAKYGNTPGLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQ
                     FTDTGGFTDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAM
                     IERNWPDILRIAATIAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWI
                     LDAELQRRAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIF
                     WNTMKLGEVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
     gene            3988..4863
                     /locus_tag="RH057_003955"
     CDS             3988..4863
                     /locus_tag="RH057_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
                     /protein_id="HDV3130252.1"
                     /translation="MRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTS
                     DGCTTVAGVNAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTF
                     AVNEKGIVNTVNNIRAVYRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTL
                     GQLGDARLFSVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQ
                     WRETQPPFDAAAAKTILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIF
                     DINRDDAKFHGEVRG"
     gene            complement(5017..5832)
                     /locus_tag="RH057_003956"
     CDS             complement(5017..5832)
                     /locus_tag="RH057_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDV3130253.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(6168..7004)
                     /locus_tag="RH057_003957"
     CDS             complement(6168..7004)
                     /locus_tag="RH057_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDV3130254.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(7004..7807)
                     /locus_tag="RH057_003958"
                     /note="aph(3'')-Ib"
     CDS             complement(7004..7807)
                     /locus_tag="RH057_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDV3130255.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            8093..>8602
                     /locus_tag="RH057_003959"
     CDS             8093..>8602
                     /locus_tag="RH057_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISVsa5 family transposase"
                     /protein_id="HDV3130256.1"
                     /translation="MCELDILHDSLYQFCPELHLKRLNSLTLACHALLDCKTLTLTEL
                     GRNLPTKARTKHNIKRIDRLLGNRHLHKERLAVYRWHASFICSGNTMPIVLVDWSDIR
                     EQKRLMVLRASVALHGRSVTLYEKAFPLSEQCSKKAHDQFLADLASILPSNTTPLIVS
                     DAGFKVPWYK"
BASE COUNT         1978 a         2431 c         2313 g         1880 t
ORIGIN      
        1 tggtgataaa cttatcatcc ccttttgctg atggagctgc acatgaaccc attcaaaggc
       61 cggcattttc agcgtgacat cattctgtgg gccgtacgct ggtactgcaa atacggcatc
      121 agttaccgtg agctgcagga gatgctggct gaacgcggag tgaatgtcga tcactccacg
      181 atttaccgct gggttcagcg ttatgcgcct gaaatggaaa aacggctgcg ctggtactgg
      241 cgtaaccctt ccgatctttg cccgtggcac atggatgaaa cctacgtgaa ggtcaatggc
      301 cgctgggcgt atctgtaccg ggccgtcgac agccggggcc gcactgtcga tttttatctc
      361 tcctcccgtc gtaacagcaa agctgcatac cggtttctgg gtaaaatcct caacaacgtg
      421 aagaagtggc agatcccacg attcatcaac acggataaag cgcccgccta tggtcgcgcg
      481 cttgctctgc tcaaacgcga aggccggtgc ccgtctgacg ttgaacaccg acagattaag
      541 taccggaaca acgtgattga atgcgatcat ggcaaactga aacggataat caacgccacg
      601 ctgggattta aatccatgaa gacggcttac gccaccatca aaggtattga ggtgatgcgt
      661 gcactacgca aaggccaggc ctcagcattt tattatggtg atcccctggg cgaaatgcgc
      721 ctggtaagca gagtttttga aatgtaaggc ctttgaataa gacaaaaggc tgcctcatcg
      781 ctaactttgc aacagtgcca tcatagatat tgtcccgttc gacacctgcg gcgcgcaagg
      841 cgtcgtgctg caggtcgaga gactgcgagc catcggcttt ggagacgcgg gcatatccga
      901 tcagcatgta tcacaaacgt tggtttgagg cggcgcttcg gccacgattg cattgacctc
      961 tggaagtgta tctcaaccag cttcataaac aaagcgtctt gaacgctatc agattttgaa
     1021 aaaggaacat gtatgccgcg tcgcgtcact ctaaccgatc ggcagaaaga cgcgctgttg
     1081 cgcttgccga cttcacagac ggatttgctc aagcactata cgctgagtga tgaagacctt
     1141 gggcatatca ggctgcgtcg gcgcgctcac aacaggttcg gcttcgccct gcaattgtgt
     1201 gtcctgcgct atcccggccg ggtgctggct ccaggcgaac tgatccctgc agaggtcatc
     1261 gaatttatcg gagcgcagct tggcctgggt gccgacgatc tcgtagacta tgctgcccgc
     1321 gaggaaacac ggcacgagca tcttgccgag ttacgggggc tctacggctt ccgcaccttc
     1381 tccggacgtg gtgcgagcga gctgaaggaa tggttgttcc gagaagccga gatggcggtg
     1441 tcgaacgagg atatcgcccg tcgcttcgta gccgagtgcc gacgcacccg cactgtcctt
     1501 cccgcgacat ccacgatcga gcggctttgt gccgcggctc tcgtcgatgc cgagcgacgc
     1561 atcgagacga ggatcgccag tcggctgcct atgtcgatcc gagaacagtt gctggcattg
     1621 ctcgaggaga cggctgatga tcgggtgacc cgttttgtgt ggctgcgcca gttcgagcct
     1681 ggctcgaact cttcgtcggc caaccggctg ctcgaccggc tcgaatatct gcaacgcatc
     1741 gatctccccg aggatctgct tgccggcgtt cctgcccatc gggtgactcg tctgcgcagg
     1801 cagggtgaac ggtattatgc cgacggcatg cgcgatctcc cggaggacag gcggcttgcg
     1861 atcttggctg tttgcgtctc ggaatggcag gcgatgttgg ccgacgcagt ggtcgaaacc
     1921 cacgaccgga tcgtcggccg tctctaccgt gcttcggagc gtatttgcca tgcaaaggtc
     1981 gcagacgaag cgggggtggt gcgtgacacc ctgaaatcct tcgccgagat cgggggcgcc
     2041 ctggtcgatg cacaggatga tggccagccg ctgggcgatg tcatcgcgag tgggtcaggg
     2101 tgggacggct taaaaaccct tgttgcaatg gcaaccaggc tgaccgccac catggccgac
     2161 gatccgctca atcatgtgct cgacggttat caccgcttcc gccgatacgc tccacgcatg
     2221 ttgcgcctgc tcgatctgcg agctgcgccc gttgcactgc cgcttctgga agcggtgacg
     2281 gcccttcgta ccggtttgaa cgatgccgcg atgaccagct tcttgcggcc cagctcgaaa
     2341 tggcatcgcc accttcgggc ccagagggct ggcgacgctc gcctatggga gatcgcggtg
     2401 ctgttccatc tgcgcgatgc gttccgctcc ggagatgtct ggcttactag gtcccggcgc
     2461 tatggcgatc tgaaacacgc actcgttccg gcacaatcca tcgcggaagg cggtcgtctc
     2521 gctgtgccat tgcggccgga ggaatggctg gcagaccggc aagctcgcct cgacatgcgg
     2581 ttgcgcgagc ttggccgtgc cgctcgcgca ggcacgatcc cgggcgggtc gattgaaaac
     2641 ggcgttctgc atatcgagaa actcgaagcc gccgcgccga caggcgccga agatctggtg
     2701 ctcgatctct acaagcagat cccgcccacg cgcatcaccg atctcctgct ggaggtggat
     2761 gcggcgaccg gcttcaccga agcgttcacc catctgcgca caggagcacc ctgcgctgac
     2821 cggatcgggc taatgaacgt tatcttggcg gaagggatca acctcggctt gcgcaaaatg
     2881 gcggatgcga caaacaccca caccttctgg gaattgatcc gcattggacg gtggcatgtc
     2941 gagggcgaag cctatgaccg ggcgctggcc atggtggtcg aggcacaggc agcgttaccc
     3001 atggcccggt tctggggcat gggcacgtcg gcttcgagcg acggacagtt cttcgtcgct
     3061 acagagcaag gtgaggccat gaacctggtc aacgcgaaat atggcaatac cccgggcctg
     3121 aaagcctata gccacgtctc cgaccaatat gcgccgttcg caacccaggt gattcctgca
     3181 acggcaagcg aagcgcctta catcctcgat ggcctgctga tgaacgatgc tggacgccat
     3241 atccgcgagc agttcaccga cacgggcggc ttcaccgatc acgtctttgc cgcatgtgcc
     3301 attctcggct accggttcgc tccgcgcatc cgcgacctgc catccaaacg gctctacgcg
     3361 ttcaatccgt cggccgcccc ggcgcacctg cgagcgttga tcggcggaaa ggtcaaccaa
     3421 gccatgatcg agcgcaattg gcccgacatc ctgcgcatcg ccgccaccat tgctgccggg
     3481 accgtcgcgc caagccagat tctgcggaaa ctcgcctcct atccgcggca gaacgagctc
     3541 gcgacagccc tgcgggaagt cggtcgcgtc gagcgcaccc tgttcatgat cgactggatt
     3601 ctggatgccg aactccaacg gcgtgcccag atcgggctca acaaaggcga agctcatcat
     3661 gcgctgaagc gggcaatcag cttccaccgc cgcggtgaaa tccgcgaccg ttccgccgaa
     3721 ggccagcatt accgcatcgc cggcatgaat ctgctcgccg ccatcatcat cttctggaac
     3781 accatgaagc tcggcgaggt cgttgcaaac cagaaacgcg atggaaagct gctatcgccc
     3841 gatctcttgg cccatgtttc gccgctcgga tgggaacaca tcaatctcac cggagaatat
     3901 cgctggccaa agccttagcg taggattccg ccccctcccg caaacgaccc cgtgatgacc
     3961 ctgcaggcat cccctgagac ccggttaatg cgcgccgagg tgcagcggca ttatccgcac
     4021 atctggcagg agctggaggg gctggcgcag ggactggagg ccactgttga tcaggtcttt
     4081 gcctggaact gccgtggcga tctggtgcgc tcaacgtcgg atggctgtac gacggtggcc
     4141 ggggtaaacg ccgcaggcga gcggattatc gcgcataatg aagacggttt tcctcagtta
     4201 cgggatgaat gcttgctggc taccattact ccgagtcgcg gtctgagctt tacgagtttt
     4261 gcctatccgg ggtcgatttg cggccacacc tttgcggtaa atgaaaaagg tatcgttaac
     4321 acggtgaata acatccgtgc agtgtatcgt ccgcagggat taccgcgtca gatcctggct
     4381 cgcgcctcgc tgaatgcgaa caccctcgac gaggccatca ctatacttac cggccagccg
     4441 cgatccgggg cgtttcacca tacgctcggg cagctgggcg atgcgcggct gttcagcgtt
     4501 gaggccaccg gtcaaggctg ttccgttcta cccctgacga cggtaaccgg gcatgccaac
     4561 catctggtgc atgcggcact ggctggcgtt gagcagatcg ttaccgacag ttcagcgtcg
     4621 cggcagttac gcctcgtgca gtggcgggaa acgcaacctc cgtttgacgc cgcggcggca
     4681 aaaaccattc tctcagatac ccatgacgca gaactgccaa tttaccggct ggctgccgac
     4741 gatccggatg aggagaacac cctcgccacg gcagttttca ccctcgatgc caaccacgtc
     4801 aggtggcaaa ttttcgacat taaccgcgac gatgctaaat ttcatggaga agtgcgtggg
     4861 tgaaatagcc ggtcgtcagt cataaagggc aggggagtac cgttggcccg gcgttcgata
     4921 gtaccgtccg gatactgcca gatatcggcg tccagggaga tgtcctgaag cgcggtgtcc
     4981 ggaatttcag gtttgtgtct ctacaaagac taactatcag aaaaactcat cgagcatcaa
     5041 atgaaactgc aatttattca tatcaggatt atcaatacca tatttttgaa aaagccgttt
     5101 ctgtaatgaa ggagaaaatt caccgaggca gttccatagg atggcaagat cctggtatcg
     5161 gtctgcgatt ccgactcgtc caacatcaat acaacctatt aatttcccct cgtcaaaaat
     5221 aaggttatca agtgagaaat caccatgagt gacgactgaa tccggtgaga atggcaaaag
     5281 cttatgcatt tctttccaga cttgttcaac aggccagcca ttacgctcgt catcaaaatc
     5341 actcgcatca accaaaccgt tattcattcg tgattgcgcc tgagcgagac gaaatacgcg
     5401 atcgctgtta aaaggacaat tactaacagg aatcgaatgc aaccggcgca ggaacgctgc
     5461 cagcgcatca acaatatttt cacctgaatc aggatattct tctaatacct ggaatgctgt
     5521 tttcccgggg agcgcagtgg tgagtaacca tgcatcatca ggagtacgga taaaatgctt
     5581 gatggtcgga agaggcataa atgccgtcag ccagtttagt ctgaccatct catctgtaac
     5641 atcattggca acgctacctt tgccatgttt cagaaacaac tctggcgcat cgggcttccc
     5701 atacaatcga tagattgtcg cacctgattg cccgacatta tcgcgagccc atttataccc
     5761 atataaatca gcatccaggt tggaatttaa tcgcggcctc gagcaagacg tttcccgttg
     5821 aatatggctc ataacacccc ttgtattact gtttatgtaa gcagacagtt ttattgttca
     5881 tgatgatata tttttatctt gtgcaatgta acatcagaga ttttgagaca caacgtggat
     5941 ttgttgaata aatcgaactt ttgctgagtt gaaggatcag atcacgcatc ttcccgacaa
     6001 cgcagaccgt tccgtggcaa agcaaaagtt caaaatcacc aactggccca cctacaataa
     6061 aggggtcgtt tgcgggagag ggcgaaatcc tacgctaagg ctttggccaa cgatattctc
     6121 cggtaagatt gatgtgttcc caggggatag gagaagtcgc ttgatatcta gtatgacgtc
     6181 tgtcgcacct gcttgatcgc ggccgcgata gctagatcgc gttgctcctc ttctccatcc
     6241 gcgttccaag ctgcggaaag gcacccataa gcgtacgcct ggtcgagcag gcgacgcgga
     6301 tcgacgtcca gcgcacgaga gaatgcgtcc gccatctgtg caatgcgtct aggatcgaga
     6361 caaaggtcgt ctctgtcagc cggatcgtag aacatattgg cggcgccaaa gcccacttca
     6421 ccgaccagac cgacgggatc tatcaccagc cagccgcgac tggagaacat gatgttttca
     6481 tgatgcagat cgccatgtag cccacgcagt tccgaggcat tgctcatcat ttgatcggct
     6541 ataatcgccg cgtggacgta gtcagtttga caacctgcgt tttgatcatc gcgcgcccgc
     6601 tgaaacaaag ctgcaaagcg atcccggatc gggagaaggg cagaaggcag gggttcctca
     6661 gatgcggcat acagcttcgc cattagttcc gctgcaattt cggtcgcctg gtagtcgccg
     6721 tgctcggcaa cgatgtgaga gagcattcgc tccccggcat attcgagcaa catcagattg
     6781 ttctcacgac cgagcaaccg gactgctccc ctcccattgc gccataccag atagtcggcc
     6841 ccgcgcagtt catcagcaat gtcttctata ggtttcaatc ccttgacgat tgcaggagtc
     6901 ccgtctggca atgaaacttt ccaaacgagg ctggaaaagg tgtccgcaat gagaacaggt
     6961 tgcgaaacgt gccaatgagc aggaaaaaca ggcggcatga acatcaaccc caagtcagag
     7021 ggtccaatcg cagatagaag gcaaggcgtt cgcggtcggg ggcttcgatc cccaatacat
     7081 tgaataggac agcgaaggcg cgctctgctt catctggcgc tgcccagttc tcttcggcgt
     7141 tagcaatcat gagtgccaaa tcggcatagc gatctgctgt tccgagccgc ccaaggtcga
     7201 tcagacccgt gcattgaaga gttttagggt ccaccatgaa gttcggcatg cagggatcac
     7261 catggcaaac aaccatatcg gtgcgctctt ggtcgagccg caccggtagc tctcgttcga
     7321 cacgagccaa aagatcgagc tgcggcgtac tcttgtcctc gtccggtaag aagtcgggat
     7381 tgacggcatt gcgggacacc acatcaacgg cgcgtccgaa cattcgcgac agcctgcgct
     7441 caaacggaca ttgatcaacc gataggctgt gaacagcgcc aagttgctgc cccattgacg
     7501 gccacgcttt gagcaaatcc gctccagaca gatcagccgc cggtactccc ggaattgccg
     7561 ttatcaccaa gcatgcaccc tcctgttcct cctgccagtt gatcacctcg gggcaagcca
     7621 cacctcgacc tttgagccaa atgaggcggt cacgctctcc agcgagctca ccgcggcggg
     7681 aagcaggtgc gattttcgcg aaggcatgcc cgtcaccacg tcgaaaaaca aaatcaccag
     7741 attctccgcc tctgacaggc aaccagtcag aatgcgattc accaaaaaaa atattagttc
     7801 gattcaatgg aggttccttc agttttctga tgaagcgcgg aggtggctca acctgcgaaa
     7861 agaaacgagt tgctacgtaa gtccgagaac atgctttcca tggtcttgca ccggttgtgt
     7921 tccggtctat ctctcatttt aagcggcttt tttctcaaat gccaccggcg atttccagcc
     7981 gagtgctgat gaatccccta atgattttta tcaaaatcat taagttaagg tagatacaca
     8041 tcttgtcata tgatcaaatg gtttcgccaa aaatcaataa tcagacaaca aaatgtgcga
     8101 actcgatatt ttacacgact ctctttacca attctgcccc gaattacact taaaacgact
     8161 caacagctta acgttggctt gccacgcctt acttgactgt aaaactctca ctcttaccga
     8221 acttggccgt aacctgccaa ccaaagcgag aacaaaacat aacatcaaac gaatcgaccg
     8281 attgttaggt aatcgtcacc tccacaaaga gcgactcgct gtataccgtt ggcatgctag
     8341 ctttatctgt tcgggcaata cgatgcccat tgtacttgtt gactggtctg atatccgtga
     8401 gcaaaaacgg cttatggtat tgcgagcttc agtcgcacta cacggtcgtt ctgttactct
     8461 ttatgagaaa gcgttcccgc tttcagagca atgttcaaag aaagctcatg accaatttct
     8521 agccgacctt gcgagcattc taccgagtaa caccacaccg ctcattgtca gtgatgctgg
     8581 ctttaaagtg ccatggtata aa
//
LOCUS       DAPHDW010000170         8595 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.173, whole genome shotgun sequence.
ACCESSION   DAPHDW010000170 DAPHDW010000000
VERSION     DAPHDW010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8595
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.173"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(127..203)
                     /locus_tag="RH057_003960"
     tRNA            complement(127..203)
                     /locus_tag="RH057_003960"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(167..169),aa:Asp,seq:gtc)
     gene            complement(336..1067)
                     /gene="dnaQ"
                     /locus_tag="RH057_003961"
     CDS             complement(336..1067)
                     /gene="dnaQ"
                     /locus_tag="RH057_003961"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HDV3130257.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVEKKGGSCLWRA"
     gene            1132..1599
                     /gene="rnhA"
                     /locus_tag="RH057_003962"
     CDS             1132..1599
                     /gene="rnhA"
                     /locus_tag="RH057_003962"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HDV3130258.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            complement(1596..2318)
                     /locus_tag="RH057_003963"
     CDS             complement(1596..2318)
                     /locus_tag="RH057_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV3130259.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDVCLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            2352..3107
                     /gene="gloB"
                     /locus_tag="RH057_003964"
     CDS             2352..3107
                     /gene="gloB"
                     /locus_tag="RH057_003964"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HDV3130260.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLKAIAANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPPETQDKGTTQVVKDGETAFVLGHEF
                     SVIATPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTASQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSILPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINVINEETLLQQPEERFAWLRSKKDRF"
     gene            3179..4537
                     /gene="mltD"
                     /locus_tag="RH057_003965"
     CDS             3179..4537
                     /gene="mltD"
                     /locus_tag="RH057_003965"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HDV3130261.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDGDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAD
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAGSSAQR
                     LANNSDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMP
                     DS"
     gene            complement(4585..5355)
                     /locus_tag="RH057_003966"
     CDS             complement(4585..5355)
                     /locus_tag="RH057_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HDV3130262.1"
                     /translation="MTTQSHHDHVEKQFSSQASEYLTSTVHASGRDLQRLAVRLADYP
                     GASVLDMGCGAGHASFVAAQNVSTVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFSSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(5433..6233)
                     /locus_tag="RH057_003967"
     CDS             complement(5433..6233)
                     /locus_tag="RH057_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HDV3130263.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            6474..7388
                     /locus_tag="RH057_003968"
     CDS             6474..7388
                     /locus_tag="RH057_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3130264.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHICLGFTEPASLNTWPIARSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHVKTAPGGAVREA"
     gene            complement(7385..8188)
                     /gene="dkgB"
                     /locus_tag="RH057_003969"
     CDS             complement(7385..8188)
                     /gene="dkgB"
                     /locus_tag="RH057_003969"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HDV3130265.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKTIAAVGAENIATNQIELSPYLQ
                     NRKVVVWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNATPAQVILAWAMGEGYSV
                     IPSSTKRENLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
     gene            complement(8351..8427)
                     /locus_tag="RH057_003970"
     tRNA            complement(8351..8427)
                     /locus_tag="RH057_003970"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8391..8393),aa:Asp,seq:gtc)
BASE COUNT         2275 a         2075 c         2086 g         2159 t
ORIGIN      
        1 atatgtgatt aacacattta cagcataaca aaaccataga ccgaagaaat agcgggattt
       61 aaatgcaaaa aaaaccgcaa aattaaaaat cttgcggttc tctgaactca ttttcatgag
      121 tgaatttggc ggaacggacg ggactcgaac ccgcgacccc ctgcgtgaca ggcaggtatt
      181 ctaaccgact gaactaccgc tccgcgttgt gttccgttgg gaacgaggcg aatattacgg
      241 attgcctcac ccttcgtcaa cggtttttct catcttttga atcgtttgct gcaaaaatcg
      301 cccaagccgc tatttttagc gccttttaca ggtatttatg cccgccagag gcagcttccg
      361 cccttcttct ccaccagatc aagacgggct tcatgagctg caagctcttc atctgtcgca
      421 aaaacaacgc gtaacttact tgcctgacgt acaatgcgct gaattgttgc ttcaccttgt
      481 tgctgctgtg tctctccttc catcgcaaaa gccatcgacg tttgaccacc ggtcatcgcc
      541 agataaactt ccgcaaggat ctgtgcatcg agtaatgccc cgtgtagcgt tcgtttactg
      601 ttatctattt cgtagcgggc acataacgca tcgaggctgt tgcgcttacc aggaaacatt
      661 ttcctcgcca ccgcaaggct atcggtgacc ttacagaagg tattggtctt cggaatatcg
      721 cgcttaagca acgaaaactc gtagtccata aagccgatat cgaacgctgc gttatggatc
      781 accaactccg cgccgcggat atagtccatg aactcatcgg ctacctcggc aaacgtgggc
      841 ttatcgagca aaaattcatc ggcaatacca tgtacgccaa aggcttccgg atccaccagc
      901 cgatcgggtt tgagataaac atggaagtta ttgcccgtca ggcgacggtt caccacttca
      961 acggcaccaa tctcaatgat cttgtggcct tcatagtgcg caccaatctg gttcataccg
     1021 gtggtttcgg tatcgagaac gatctggcgt gtaattgcag tgctcatagc ggtcatttat
     1081 gtcagacttg tcgttttaca gttcgattca attacaggaa gtctaccaga gatgcttaaa
     1141 caggtagaaa ttttcaccga tggttcgtgt ctgggtaatc ctggacctgg gggttacggc
     1201 gctattttac gctatcgcgg acgcgagaaa acctttagcg caggctacac ccgcaccacc
     1261 aacaaccgta tggagttgat ggccgctatt gtcgcgctgg aggcgttaaa agaacattgc
     1321 gaagtcattt tgagtaccga cagccagtat gtccgccagg ggatcactca gtggatccat
     1381 aactggaaaa aacgtggctg gaaaacagca gacaaaaaac cagtaaaaaa tgtcgatctc
     1441 tggcaacgtc ttgatgctgc attggggcag catcaaatca aatgggaatg ggttaaaggc
     1501 catgccggac acccggaaaa cgaacgttgc gatgaactgg ctcgtgccgc agcgatgaat
     1561 cccacactgg aagatacggg ctatcaagtc gaagtttaag cctgtggttt acgacattgc
     1621 cgggtggctc caaccgcctg gcgaattcgt ggcttgtttt tactctgttt catcgggttt
     1681 agcgttaaag gaatagtccg tttccgggca acaataagtt gtaagcaacc aagcgcagga
     1741 atatgcgcat tcaatagttt tcctccgtgt ttgttccacg ggagaacgtg gaaacggctg
     1801 gcgtgcagca cttcaaaatt caacaaagag agccagtcca gttgccgcat cagagtaaac
     1861 atccggctgt tatagggcga ggtttttcgc aataccggca caagtttgcg taatcccatt
     1921 aaactgatgg gattgaagcc actaatcacc agccagccat catcaatcag tacccgatcg
     1981 gcttcacgca ataaacgatg cggatcggtg caccacggca aggtatgtgc cagtagacaa
     2041 acatcaacgg atttatcggc aaaaggaaga tgaagtggat ctgcctgcac ctggacgggc
     2101 attccttgcg cagaaacatt cacttgatga gaaaccgcgc acgcttcgca attgatttct
     2161 gcgcttaaat tgccaatctt aagcagatga aaaccataca ttttagtgaa ccacgggttg
     2221 agctggcgct ccagcgcctt gcgataaagc tctccccagg gcaaatcgcc ccagcaatca
     2281 ggagccacga cagtttgagg gactcttgcc ggtttcatca caaccttccg tttcacactg
     2341 agaggtaatc tatgaatctt aacagtattc ccgcctttga tgacaattac atctgggttt
     2401 tgaatgatga agcaggtcgc tgcctgattg tcgatcccgg agacgcagag ccggtattaa
     2461 aagccattgc cgccaataac tggcaaccag aggccatatt tctcacccac catcaccacg
     2521 atcacgttgg cggcgtaaaa gaactggtgg aaaagtttcc gcaaattgtg gtgtatggtc
     2581 caccagagac acaagataag ggaacaacac aggtagtcaa agatggcgaa actgccttcg
     2641 ttttggggca tgaatttagt gtaattgcca cgccgggtca cactttagga catatctgtt
     2701 acttcagtaa accttatcta ttttgcggcg acacgctgtt ttctggtggg tgtggtcggt
     2761 tgtttgaagg gacagcatca caaatgtatc aatcacttaa aaagttaagt gcgttacctg
     2821 acgatacatt ggtatgttgt gctcatgaat atacgttatc aaatatgaag tttgctttga
     2881 gtattcttcc gcacgatttg tccataaatg attattatcg taaagttaag gagttacggg
     2941 caaaaaatca aataacacta cccgtaattc tgaaaaatga gcggcaaatt aatgtttttt
     3001 taagaacgga agatattgat ttaattaatg taattaatga agaaacatta ttgcaacaac
     3061 ctgaagagcg ttttgcatgg ttaaggtcaa agaaagatag gttctgataa aactttcttg
     3121 tcatcggctc cgttcgccgt tatgatcggt cgtcttttaa gcaactattg acacacacat
     3181 gaaggcaaaa gcgatattac tcgcctctgt cctgctcgtg ggttgccaga gtaccggcaa
     3241 cgttcaacag cacgcacaga gcctttctgc agctggtcaa ggggaagcag caaagtttac
     3301 aagtcaggca cgatggatgg acgatgggac gtctatcgcg ccagatggtg acttgtgggc
     3361 tttcattggc gacgagctaa agatgggaat tccggaaaat gaccggattc gcgaacagaa
     3421 acagaaatat ttacgcaata agagctatct ccacgatgta actttacggg cagagccgta
     3481 tatgtactgg atagcagggc aagttaaaaa acgtaacatg cctatggaac tggtactact
     3541 acccatagtg gagagcgctt ttgatcctca cgcaacgtct ggcgccaatg ccgcaggcat
     3601 ctggcagatc attccgagca cggggcgcaa ttatggtttg aaacagaccc gcaattatga
     3661 cgcgcgtcgc gatgttgttg cttcaacaac tgccgcgctg aatatgatgc agcgtctgaa
     3721 caaaatgttt gacggcgact ggcttctgac cgtagcggct tataacagcg gcgaaggtcg
     3781 ggtcatgaag gcaattaaaa cgaacaaagc gcgtgggaaa tccacggact tctggtcgtt
     3841 accgttgccg caggaaacga agcagtacgt gcctaaaatg ctggcattga gtgatattct
     3901 caaaaacagc aagcgttatg gcgtacgtct gccaacgacc gatgaaagcc gtgctctggc
     3961 gcgtgtgcac ctgagcagcc cggttgaaat ggcgaaggtt gcagatatgg cggggatttc
     4021 cgtcagcaag ctgaagacat tcaacgctgg cgtgaaaggc tccacgctgg gcgcaagtgg
     4081 gccgcagtac gtgatggtgc caaagaagca tgcagatcaa ctgcgtgaat ctctcgcttc
     4141 aggcgaaatt gctgctgtac agtcgacgct ggttgccgac aatacgccgc ttaacagccg
     4201 tgtttacacc gtacgctctg gcgacacgct ttcaagcatc gcttcacgtc tcggcgtaag
     4261 caccaaagat ttgcagcagt ggaacaaact gcgcggatct aagctgaagc caggccaaag
     4321 tttgacgatt ggtgcaggca gtagcgcaca gcgactggca aacaacagcg atagcattac
     4381 gtatcgtgtg cgcaaaggcg attcgctttc aagcattgct aaacgccacg gcgtgaacat
     4441 caaagatgtg atgcgttgga acagcgacac tgcgaatctg caaccaggcg acaagctgac
     4501 gttgtttgtg aaaaacaaca acatgccaga ttcctgacaa accagttaat aaaaaggcac
     4561 cgattccccc ggtgcctttt ttatttatgc cgctttatgt gcttctacca tgatgatatc
     4621 actggtgaaa gagccgtcat tctgcaaggc aaaatacgtt ctcacctctg tcgatgcgct
     4681 ctgttggtaa atgcgaatag cgtctactaa cgcttctggc gtacgcattc tcgcgaccca
     4741 tgaagaaaat tccagcggta acttatctgt aattaaatta tcaactatca gattggcttc
     4801 attgattaac ctcaaccact caccgctggc gtagtttcgt acgtgagagg tatcgcgtaa
     4861 tgcttctacc gtctgtaacc agatgtcgcg cactggatga cccggagaca ttacgtccat
     4921 cacaatcatc ctaccgccag gtttcaatat ccggttcact tcacgcagtg ctgcaccaac
     4981 atcatgccag tgatgggcag aataacggct gataacaata tcaaatgcgt tatcggcaaa
     5041 tggcagactt tcggcatatc cctggcgggt ggtgatattt ttcagttgcc gggcttcggc
     5101 agcttgtgcc acgacatcca gcatttgagc agataagtca tacgccacca ccgtgctcac
     5161 gttttgcgca gcgacaaagc tggcatgccc tgctccgcag cccatatcaa gtacacttgc
     5221 accaggataa tcagccagac gcaccgccag gcgctgtaaa tctcgcccgg atgcatgtac
     5281 ggtactggtt aaatactcac tggcctgcga gctaaactgc ttttctacgt ggtcatggtg
     5341 ggattgtgtt gtcattgcgc tgtccttttg ttgtttgagt ataaaaggac agcgccaaac
     5401 gttggtctgc cctgtggcag acctgacata ccttatttat caggcttgcc gggactgaat
     5461 tcaacgagta gcggattgtg atcggaagcg cgcgtaacca atacagaagc ttcgctgacg
     5521 ttcagaccac ggtagaaaac aaaatcgagc gggcggccaa acgcccgacg gcgctgatca
     5581 tcggtaaaac gcacctggcg cagcgacatt tcccgcgcaa agcgatataa cgcgttcatc
     5641 cttctacggc tccaggcatt gaaatctcct gccataatga ccgggccgct gtggtgagct
     5701 atctgatcac caataggaag taactgctta ctatagacat ccacgcccag actgaagttg
     5761 acggcgtgta tattaaccac catcaacagg cgggtgtcag gtaatggata gaccgtcacc
     5821 agtgccgact tcgccagacg caaaatgggt tctcgttcac gtaacgggca gcaatacact
     5881 ggatgtgccg ccgaaagggt cattacgcca gaaggatgtt gtggcagtac gaaagcgggt
     5941 acctgatcgg cggcaagata gttagcggtc gcaaactgta ctaactctgg cgttgtctgc
     6001 gcttcctgca ataacaccag atgtgcatct ttgccgtagt tctttaatac cgacaaccat
     6061 tcagcgcgtt gctgtttgta tatgttccac accaggatcc gaatacgctc ttcggtactt
     6121 aacggttccc caggtggtaa tgcctggccg atgctcgcaa aagaccccgg cggtaagatc
     6181 ctttccgcag gttgtccggc aacatagcgc atggcatagg tgttttttcg cacttttgac
     6241 tttgaaacct ctattacgtc aacccttccg cactcgggag ggttctactg ttatagggat
     6301 tttagctcac tctttcaacg agcaattgta tattgttatg taagcaagtg cttacaccgg
     6361 tcagaatttt gctcattgtt gagaacatta aatcggatga catatcacgc atcctgatgg
     6421 tttaatgaca aataaattca aaatatctct tctattttgc aacaggagca aaaatgaaag
     6481 ccacgtcgga agaactcgcc atttttgttt cggtcgtcga aagcggcagc tttagccggg
     6541 cagcggaaca attagggcaa gcaaactcag cggtaagccg ggcagtgaaa aagctggaga
     6601 tgaaacttgg cgttagcctg cttaatcgga ccacgcgaca acttagcctg acggaagaag
     6661 gcgagcgtta ttttcgtcgc gtacagtcaa ttttgcagga gatggcagcg gcagaatcag
     6721 aaattatgga gacgcgtaat acaccgcgtg gactgttgcg catcgatgcc gcaactccag
     6781 tggtgctgca ctttctgatg ccgttgatta agcctttccg tgaacgctat ccggaagtca
     6841 ctttgtcgct agtctcctcc gaaacgatta ttaatttgat cgaaagaaaa gttgatgtcg
     6901 cgatacgcgc cggtacgtta acggattcca gcttacgtgc caggccgtta tttaatagtt
     6961 atcgaaaaat tatcgcctcc cccgattata tttcccgcta cgggaagcca gaaacgatcg
     7021 acgatttaaa gcaacatatt tgcctgggat tcactgaacc cgcttccctc aatacctggc
     7081 cgatagcccg tagcgatgga caattacatg aggtgaagta cggtttgtca tccaatagtg
     7141 gggaaacact gaaacagctt tgcctgagtg gcaacgggat tgcgtgtttg tccgactaca
     7201 tgatcgacaa agaaatcgct cgcggagaat tggtggagtt aatggcagat aaagtgttgc
     7261 cagtggaaat gccattcagt gcagtctatt acagcgaccg tgcggtaagt acgcgcatcc
     7321 gggcttttat cgatttcctt agcgagcatg taaaaacagc tcccggagga gctgtcagag
     7381 aggcttaatc ccattcagga gccagacctt ccgggctaac caggcggtcg ttgcaatcca
     7441 gtgcggcgat cgctttttta tcttcggcat caagctgtaa attttgtgcc ttaagattac
     7501 tttccaggtt ttcacgttta gtagaagaag gaattactga gtaaccttcc cccatagccc
     7561 acgccagaat cacttgtgcc ggagtcgcat tgtgtttagc tgcgatacga gcaataacct
     7621 catctttcag ggccttacca tacgccagcg tcatatagga agtaatatgg atgccgtgct
     7681 gtttagccca gacaaccact ttacggtttt gcagataagg agagagttca atctggttag
     7741 tagcgatgtt ttcagcgcca acagcagcaa tcgttttttc catcaacggg atcgtgaagt
     7801 tggaaatacc gatctcacgt gtcagacctt gttttttggc ttccagcagc gcctgcataa
     7861 actcttcaac agagacttca tcgtttggtg acggccagtg gattagcgtc agatcaacat
     7921 aatcggtacg caatttttgc aggctctctt tcagacttgg gatcaatttg tctttgctga
     7981 gattttcaat ccagatttta gtggtgatgt agagttcatg acgtggcacg ccactttctg
     8041 caatcgcctg acctactgcg gcttcgttat catagatttg tgcggtatca attgcgcgat
     8101 aaccaagttc aagcgccgtt ttcacagatg aaataacaac gtcgtctttc agacggaaag
     8161 tacctaaacc aaatgcaggg atagccataa tatgcctctt ttaatgctat tttcgttaac
     8221 tgaggtgatt atgggcttca gactgttgcg gaaaaagagc gtaaaacgca gaggattttt
     8281 gcgattctgg caataataga tatccagacg aaaaaaaacc tcgagcgtta actcgaggct
     8341 tcttaataag tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg
     8401 tattctaacc gactgaacta ccgctccacc gaattctttt acaaccaccg gttttatgac
     8461 cggcttactg cttaatttga tgcctggcag tttatgaatc acttcgtgat tcacccttcg
     8521 ggccgttgct tcgcaacgtt caaaagcttt cgcttttgtc ctactctctt atcgagtagt
     8581 gaactgcctc aaaga
//
LOCUS       DAPHDW010000171         8455 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.174, whole genome shotgun sequence.
ACCESSION   DAPHDW010000171 DAPHDW010000000
VERSION     DAPHDW010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.174"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(60..1652)
                     /locus_tag="RH057_003971"
     CDS             complement(60..1652)
                     /locus_tag="RH057_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HDV3130266.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFADPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            1871..2791
                     /gene="ldtB"
                     /locus_tag="RH057_003972"
     CDS             1871..2791
                     /gene="ldtB"
                     /locus_tag="RH057_003972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HDV3130267.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVERKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(2850..3968)
                     /locus_tag="RH057_003973"
     CDS             complement(2850..3968)
                     /locus_tag="RH057_003973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HDV3130268.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPLAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWSCLGLYIVFLTALE
                     FKQELWGLVIVAAGFALLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAIGLSQVISNVPSTILLLNYVPPSLLLVWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVILPAN"
     gene            complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="RH057_003974"
     CDS             complement(3965..4432)
                     /gene="mntR"
                     /locus_tag="RH057_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HDV3130269.1"
                     /translation="MSRRAGTPTAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            4618..4746
                     /gene="mntS"
                     /locus_tag="RH057_003975"
     CDS             4618..4746
                     /gene="mntS"
                     /locus_tag="RH057_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HDV3130270.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            5018..6601
                     /gene="opgE"
                     /locus_tag="RH057_003976"
     CDS             5018..6601
                     /gene="opgE"
                     /locus_tag="RH057_003976"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3130271.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGISSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYMVGIFIFVLGVIAIRRKKESEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSDDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGSIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="RH057_003977"
     CDS             complement(6650..7165)
                     /gene="ompX"
                     /locus_tag="RH057_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HDV3130272.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(7218..7283)
                     /locus_tag="RH057_003978"
     CDS             complement(7218..7283)
                     /locus_tag="RH057_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130273.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            7518..8405
                     /gene="rhtA"
                     /locus_tag="RH057_003979"
     CDS             7518..8405
                     /gene="rhtA"
                     /locus_tag="RH057_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HDV3130274.1"
                     /translation="MPGSLRKMPVWLPIIILLVAMASIQGGASLAKSLFPLVGAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVAILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
BASE COUNT         2079 a         2244 c         2027 g         2105 t
ORIGIN      
        1 attttgcatt tgtcatcagc cgccgtcacc ccgacagtat cggggtgacg gataatcact
       61 tactcgatcc ctttactacg caggtaatct tcgtaattac cgctaaagtc gatcacgcgt
      121 tccggggtga tttccagaat gcgggtcgcc agggagctta cgaactcacg gtcgtgtgaa
      181 acaaagatca gcgtgccctg atacagttcc agcgccatgt tcagtgactc gatggattcc
      241 atatccaggt ggttggtcgg ttcgtccatg atcagaatgt tcggcttctg catcattaac
      301 ttaccaaaca gcatccgccc tttttcccca ccggaaagca ctttagctgg ctttttgatg
      361 tcgtcctggc tgaacagcaa acgaccgaga atgctgcgta ccgcctgctc gtcatcgcct
      421 tcctgcttcc actgactcat ccattcgaac acggtcagat cattttcaaa ctcatattcg
      481 tggtcctggg cgtagtaacc aatgcgcgcg ttttcagacc attttacggt gccgctgtcc
      541 ggttgcagat cgcccaccag cgttttcagc agcgttgatt taccgacgcc gttggtaccc
      601 agtaccgcaa gtttttcacc gacctccagc agcaggttga gatttttaaa cagtggaccg
      661 ttatcaaacc ctttggtcag accttccact tccagcgcgt tacggaacag tttcttatcc
      721 tgttcaaaac ggatgaacgg gttctgacgg ctggaggctt tcacctcttc cagtttgatt
      781 ttatcaatct ggcgcgcgcg cgaagttgcc tggcgagatt tcgaggcgtt ggcgctaaag
      841 cggctaacga aagattgcaa ctcagcaatc tgcgctttct tcttggcgtt atcggccagc
      901 agacgttcac gcgcctgggt cgccgccgtc atgtactcat cgtagttacc cggataaacg
      961 cgcagctcgc cgtaatccag atccgccatg tgggtacaga ccatgttaag gaagtgacgg
     1021 tcgtgcgaga tgatgatcat ggtgctgtca cgctcgttca gcacctgttc cagccagcga
     1081 atggtgtcga tgtcgaggtt gttggtcggt tcgtcgagca ggagaatatc cggatcagca
     1141 aacagcgcct gcgccagaag cacacgcagc ttccagccag gagcaacttc actcatcggg
     1201 ccgtagtgct gttccactgg aattcccacg ccaagcaaca gttcaccggc gcgagcttcc
     1261 gcagagtaac cgtccatttc gccgtatttc acttccagat cggccacttt atagccgtct
     1321 tcttcactca tttccggcaa agcatagatg cggtcgcgct cctgcttcac ttcccacaac
     1381 tctttatgcc ccatgatcac cgtatccagc acagtgaact cttcaaaggc aaactgatcc
     1441 tgacgcagct taccaatgcg ctcgttggga tcgagggaaa cgttacccag cgtcggctca
     1501 aggtcgccgc cgagaatctt cataaaggtg gatttaccac taccgttcgc gccaatcagg
     1561 ccgtaacggt tgccgccgcc gaatttgacg gaaatgtttt caaacaacgg cttactgccg
     1621 aactgcatgg tgacgttact ggaaactaac acaggcgtat cctgaaaaga gatatgacaa
     1681 accgcgcatt atgccataag tctgaaagaa gatcctgctc ctcgcttaag gtctacactc
     1741 aagccaaagg caaaaaatgt gatttcgtac acatctgatt tcactgtgag ctggaatgaa
     1801 cttataatgc gcttccaata ctctaatatt ctcaacccaa tggcctgcca ggcacaaaat
     1861 ctcgcttaac atgaatatga aattgaaaac attattcgca gcggccttcg ctgttgtcgg
     1921 cttttgcagt accgcctctg cggtaactta tcctctgcca accgacggga gtcgcctggt
     1981 tggtcagaat caggtgatca ccattcctga aggtaacact cagccgctgg agtattttgc
     2041 cgcggagtac cagatggggc tttccaatat gatggaagcg aacccgggtg tggatacctt
     2101 cctgccgaaa ggcggtactg tactgaacat tccgcagcag ctgatcctgc cggataccgt
     2161 tcatgaaggc atcgtcatta acagtgcaga gatgcgtctg tattactatc cgaaagggac
     2221 caacaccgtt atcgtgctgc cgatcggcat tggtcagtta ggcaaagata cgcctatcaa
     2281 ctggaccacc aaagttgagc gtaagaaagc aggcccgacc tggacgccga ccgccaaaat
     2341 gcacgcagag taccgcgctg cgggcgagcc gcttccggct gtcgttccgg caggtccgga
     2401 taacccgatg gggctgtatg cactctacat cggtcgcctg tacgctatcc atggcaccaa
     2461 cgccaacttc ggtatcggcc tgcgtgtaag tcatggttgt gtgcgtctgc gtaacgaaga
     2521 catcaaattc ctgttcgaga aagtaccggt cggtacccgc gtacagttta ttgatgagcc
     2581 ggtaaaagcg accaccgagc cagacggcag ccgttatatt gaagtccata acccgctgtc
     2641 taccaccgaa gcccagtttg aaggtcagga aattgtgcca attaccctga cgaagagcgt
     2701 gcaaacagtg accggtcagc cagatgttga ccaggttgtt cttgatgaag cgattaaaaa
     2761 ccgctccggg atgccggttc gtctgaatta atcttcaaaa cttaaagcaa aaggcggact
     2821 cataatccgc cttttttatt tgccagaccc tagttggccg ggagtataac taacaaaaca
     2881 tatcccacca acgccgccca caacagcatc ggtattgaat agagatggaa acgccaccag
     2941 atgcggcgat cgttcgccat acgtagcgca atcaaatttg ccagcgatcc gggtaataac
     3001 ccaaagccac ctacgtttac cgcccatacc agtaataaag acggcggcac atagttcagc
     3061 aacaatatgg tactcggcac gttactgatc acctgcgata aaccgattgc cgttaaccat
     3121 aacccgggtt cagatagatg actcacgtta cccaacacgc cttgcaacgc tggaagctgg
     3181 gtcagtaaat ggacgtcgat aaacatcgcc ataaacacca gcagcagcgt ccagtccaca
     3241 ctgagcacca cgcgacgtgc cagcagcgca aagcctgccg ccacaatcac cagtccccac
     3301 agctcttgtt tgaactccag cgccgtcaga aagacgatat acagccccag acaactccac
     3361 accagccgcg gtttccactc cggtgtttgc acccccgtat ggtattgcag cgcctttcca
     3421 gggaaacaac accagcacag gagcaggagc gtcagcatca ttgcgccagc cagcggtgcc
     3481 atttgggcaa taaatccggc aaacgaaaga ccagaacgtc cccagataag aatattttgc
     3541 gggttgccaa ttggcgtcag tagcgaacca gcgttgactg ccagcgcctc aaaaataatc
     3601 agccgattaa ccgggatctc acacagtctt tttagcgtga tagtcagcgg aacaacaata
     3661 aacagcgcga catcgttggt cagaaaggta gaaagcagcg ccgccgccag caccataaac
     3721 atcgccagcc gacgctccgt agcaaagcgg cgcaccattt tgcgccccag cacatcaaaa
     3781 taaccgctta actccacacc tttggtcagc agcatcaggc cgcttaaggt gatgatggtg
     3841 tgccagtcga tagcagcagg ccaggatttc ggtgcaaagg gcacgaaaaa gcttaatccg
     3901 ataccaacaa gaattaataa ctgaaaaaaa cgatcgcctt gcagcgtgcg taaaaaaggc
     3961 aggctcattt ggcaccgtgt ttctgggtaa acaaacgaaa agcgtcgagc gtctcttcac
     4021 taacatggtg ctccatgcct tccgcgtcgc gacgggcgat ttccggactg acgcccaaca
     4081 ccaacaagaa attttcgact atctgatgtc gctcgcggct ttcctgcgcc agcttctctc
     4141 cttctgccgt taaaaacacg ccacgccagg ggatcatttc aatcagcccc atggttgcca
     4201 gccgcttaag cattttagcc accgtcggtt gcgaaactcc cagacgagca gccatgtcca
     4261 cctgacgagc ttcccccact tccctgatca agtcagaaat cagctcaacg taatcatcaa
     4321 taagctcgcg ccgatgcgcc tctctgacct ggcggaaccc ttcaacgtgc tcttccacgt
     4381 tcactaactg cgtcactttt tttgctgttg gcgtacctgc gcgacgactc attgtgcttc
     4441 ctcattatgg tgacgcatac atagcgttct catttcgtgc gcacattgta aaccagagtt
     4501 gcgaaggtac aaaaaattaa cgttttagca atagctatat aatatagcct gtgctatatc
     4561 tgtatgtaat gcaatcatcc ctcaaggatc gacgggatta gcaagtcagg aggtcttatg
     4621 aatgagttca agaggtgtat gcgcgtgttt agtcattctc cctttaaagt acggttaatg
     4681 ctgctctcta tgttgtgcga tatggtcaac aacaaaccgc agcaagataa accttccgat
     4741 aaatagcggc gtcgcggtac gccgcttcac tcctgctttc atgcaggcat aacgcgtttt
     4801 ggtctgaaaa accccacttt ttgtcggatt tgcaatcccc ttcgcaaaag atttgttcgt
     4861 cagtagttga cctgaacggc ggctcgctct atcttcttgc agccctgcgt atattgcggc
     4921 tcgcggatgc ggaccccttt cccctcttca cgcactcttg caggtattga cccttgacgc
     4981 cagggtaagc acatggcgtt tgttacgata gtggcatatg aatttaaccc tcaaagaatc
     5041 gcttgttacc cgtagccggg tatttagccc gtggactgcg ttctactttt tacagtcgct
     5101 attaattaac ctcggcttag gttacccctt cagtttgctc tacaccgctg cgtttacggc
     5161 tattttgctt ttgctatggc gaacattgcc tcgcgtacaa aaagttctgg tcggtatcag
     5221 ttcgctggtg gcggcttgtt atttcccttt tgctcaggcc tacggcgcgc ctaacttcaa
     5281 tacattgctg gcattgcact ccaccaatat ggaagagtcg accgaaatcc tgacgatttt
     5341 tccgtggtac agctacatgg tcggcatatt tatttttgtg ctcggcgtaa tagcaatcag
     5401 gcgaaaaaaa gagagtgaaa aagcgcgctg gaataccttc gacagcctgt gtctggtatt
     5461 cagtgtggcg acattttttg ttgctcccgt gcaaaacctg gcctggggcg gcgtatttaa
     5521 actgaaagat actggctatc cggtatttcg ttttgctaag gatgtcatcg tcaataataa
     5581 cgaggtgatt gaagagcaag aacggatggc aaaactttcc ggaatgaaag atacctggac
     5641 ggtcactgcc gttaagccga agtatcagac ctatgtggtg gtgatcggtg aaagcgcgcg
     5701 tcgcgatgcc ctcggtgcct ttggcggtca ctgggacaat accccgtttg ccagcagcgt
     5761 taacggtttg atatttgctg actacattgc cgccagtggc tccacgcaga aatcgcttgg
     5821 cttaacgctc aatcgcgtag tcgatggcaa accacagttt caggataact ttgtcaccct
     5881 ggcaaatcgc gcgggcttcc agacctggtg gttttccaac cagggccaaa tcggcgaata
     5941 cgatactgct atcgccagca tcgccaaacg agcagatgaa gtgtacttcc tgaaagaagg
     6001 taattttgaa gcagataaaa ataccaaaga cgaagcgtta ctggatatga ccgctcaagt
     6061 gctggcgcaa gagcactcgc aaccgcagct gattgttcta catctgatgg gctcacatcc
     6121 gcaggcctgc gacaggacac aaggcaaata cgaaaccttt gtgcagtcga aagaaacgtc
     6181 gtgctatctc tataccatga cgcaaacgga cgatttactg cgcaagctgt acgatcagtt
     6241 acgcaacagc ggcagcagct tctcgctggt ttacttttct gaccacggtc tggcctttaa
     6301 agagcgcggc aaagacgtgc aataccttgc ccatgatgat aagtatcagc aaaatttcca
     6361 ggtgcctttt atggtcattt ccagcgacga taaagcgcat cgggtgatta aagcccgccg
     6421 ctcagccaat gacttcttag gttttttctc ccagtggacg ggaattaaag cgaaggaaat
     6481 taacatcaaa tacccgttta tatctgagaa gaaagccggg tcgatataca tcaccaactt
     6541 ccagttacag aaggtggatt acaaccatct cggaaccgat attttcgacc cgaagcctta
     6601 aaaacaaaaa tccgccccga gaggcggatt ttttatatca ccaaagtgat tagaagcggt
     6661 aaccaacacc ggcaatccag gtgcctacgt caacgctacg aatacggctc tgctcgtaag
     6721 agaagtccag agcaacgttt tccatcgggt tgaactgcag accagcaccg taggagaaac
     6781 cgtagtcgct ggtgtcgtgt ttgtaggtcg ggtattcagt ggtctggaat ttaccataac
     6841 ccacacccac tacaccgtag atgcttgccc agtcgttaat gcggtaagcc ggaccagcag
     6901 tgatgccgta gtactggttt ttgttgtagt caccagagct tgcagtacgg cttttctcgg
     6961 tgtaagtgaa agaaccgatc acacccagcg ggctgttgtc ttcttcatag cggtatttca
     7021 ggttgaaacc gcccattttg ttcatttggc cctgagcgtc gctctgtgcg taaccgccag
     7081 ttacggtaga agtcgcagct acggaagtac ctgcggtgaa agccagaact gcggccagtg
     7141 ctgaaagaca tgcaattttt ttcataacca cctcaaatgt gattcaaata agtcctaagt
     7201 tttaaatata tcaaaaatta atgggaaact cttcgcgatt tgtgatgtct aacgggccat
     7261 ttcatgtaac agaacgtttc catacaccgc tatccatcta aatttaaatc actttttcag
     7321 agaactgcgt aagtattacg catgttttcc ctgtcattca tccagattat tcctaatcac
     7381 cagactaatg attccatcaa tcctggcgca ttttagtcaa aacgggggaa aattttttca
     7441 acaaatgctc aaccagcatt gggtatatcc agtacactcc acgctttact taagtctaga
     7501 tatttgtggg agaaaggatg cctggttcat tacgtaaaat gccggtctgg ttaccaataa
     7561 tcatattgct cgttgccatg gcgtctattc agggtggagc ctcgttagct aagtcacttt
     7621 ttcctctggt gggcgcaccg ggtgtcactg cgctgcgtct ggcattagga acgctgatcc
     7681 tcatcgcgtt ctttaagcca tggcgactgc gctttgccaa agagcaacgg ttaccgctgt
     7741 tgttttacgg cgtttcgctg ggtgggatga attatctttt ttatctttct attcagacag
     7801 taccgctggg tattgcggtg gcgctggagt tcaccggacc actggcggtg gcgctgttct
     7861 cttctcgtcg cccggtagat ttcgtctggg ttgtgctggc ggttcttggt ctgtggttcc
     7921 tgctaccgct ggggcaagac gtttcccatg tcgatttaac cggctgtgcg ctggcactgg
     7981 gggccggggc ttgttgggct atttacattt taagtgggca acgcgcagga gcggaacatg
     8041 gccctgcgac ggtggcaatt ggttcgttga ttgcagcgtt aattttcgtg ccaattggag
     8101 cgcttcaggc tggtgaagca ctctggcact ggtcggttat tccattgggt ctggctgtcg
     8161 ctattctctc gaccgctctg ccttattcgc tggaaatgat tgccctcacc cgtttgccaa
     8221 cacggacatt tggtacgctg atgagcatgg aaccggcgct ggctgccgtt tccgggatga
     8281 ttttcctcgg agaaacactg acacccatac agctgctggc gctcggcgct atcatcgccg
     8341 cttcaatggg gtctacgctg acagtacgca aagagagcaa aataaaagaa ttagacatta
     8401 attaaattta catttctgca tggttatgca taaccatgca gaatttctcg ctact
//
LOCUS       DAPHDW010000172         8329 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.175, whole genome shotgun sequence.
ACCESSION   DAPHDW010000172 DAPHDW010000000
VERSION     DAPHDW010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8329
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.175"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..289
                     /locus_tag="RH057_003980"
     CDS             <1..289
                     /locus_tag="RH057_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130275.1"
                     /translation="FNTNKLTRNDNIFILFSSVDDGPVMVVSSQRLHDMLNPTKDTNW
                     NSTYIYKSRHEMLPVNLTQETLFSSKSHGKYALFPIFTASWRAHRIMNKGV"
     gene            512..1009
                     /gene="ubiC"
                     /locus_tag="RH057_003981"
     CDS             512..1009
                     /gene="ubiC"
                     /locus_tag="RH057_003981"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HDV3130276.1"
                     /translation="MSHPALTQLRALRYFKEIPALEPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            1022..1894
                     /gene="ubiA"
                     /locus_tag="RH057_003982"
     CDS             1022..1894
                     /gene="ubiA"
                     /locus_tag="RH057_003982"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HDV3130277.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWILAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(2049..4472)
                     /gene="plsB"
                     /locus_tag="RH057_003983"
     CDS             complement(2049..4472)
                     /gene="plsB"
                     /locus_tag="RH057_003983"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HDV3130278.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RLYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            4643..5011
                     /gene="dgkA"
                     /locus_tag="RH057_003984"
     CDS             4643..5011
                     /gene="dgkA"
                     /locus_tag="RH057_003984"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HDV3130279.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            5121..5729
                     /gene="lexA"
                     /locus_tag="RH057_003985"
     CDS             5121..5729
                     /gene="lexA"
                     /locus_tag="RH057_003985"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HDV3130280.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            5802..7127
                     /gene="dinF"
                     /locus_tag="RH057_003986"
     CDS             5802..7127
                     /gene="dinF"
                     /locus_tag="RH057_003986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HDV3130281.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQELADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGVWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            7243..7452
                     /locus_tag="RH057_003987"
     CDS             7243..7452
                     /locus_tag="RH057_003987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HDV3130282.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(7494..8009)
                     /gene="zur"
                     /locus_tag="RH057_003988"
     CDS             complement(7494..8009)
                     /gene="zur"
                     /locus_tag="RH057_003988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HDV3130283.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
BASE COUNT         1897 a         2025 c         2270 g         2137 t
ORIGIN      
        1 ctttaacact aataaattaa cacgaaacga taatatattt atcctgttct cctctgtcga
       61 tgatgggcct gttatggtgg taagctccca gcgcttacat gatatgttga atcctacaaa
      121 agataccaat tggaattcca cgtatatcta caaatccaga catgagatgt tgcctgttaa
      181 tcttactcag gaaacacttt tcagctccaa atctcatggt aaatatgcgc ttttccccat
      241 ttttactgcg agttggcgag ctcatcgtat aatgaataag ggtgtttaag taaaggaaaa
      301 catcaccgtt cctggcatcc tgggcggtga tgcccctatg gttgccctct ccagcgcggg
      361 catcggcaaa gcgtaaggtt caacagcgtt ttaccacttc atgcgattgt tgcgtttttg
      421 ttgcgtatta gatcacttaa tttgctttac atctcccgta aacacttttc tgcgatacaa
      481 tgcctttacg ttatgtaacg gagagttcgg catgtcacac cccgcgttaa cgcaactgcg
      541 tgcgctgcgc tattttaaag agatccctgc cctggaaccg caactgctcg actggctgtt
      601 gctggaggat tccatgacaa aacgttttga acagcaggga aaaacggtaa gcgtgacgat
      661 gatccgcgaa gggtttgtcg agcagaatga aatccccgaa gaactgccgc tgctgccgaa
      721 agagtctcgt tactggttac gtgaaatttt gttatgtgcc gatggtgaac cgtggcttgc
      781 cggtcgtacc gtcgttcctg tgtcaacgtt aagcgggccg gagctggcgt tacaaaaatt
      841 gggtaaaacg ccgttaggac gctatctgtt cacatcatcg acattaaccc gggactttat
      901 tgagataggc cgtgatgccg gactgtgggg gcgacgttcc cgcctgcgat taagcggtaa
      961 accgctgttg ctaacagaac tgtttttacc ggcgtcaccg ttgtactaag aggaaaaaaa
     1021 tatggagtgg agtctgacgc agaataagct gctggcgttt catcgcttaa tgcgtacgga
     1081 taagccaatt ggcgcgttac tgctgctctg gccaacatta tgggcgttgt gggtggcgac
     1141 accgggcgtt cctcagctct ggatcctggc ggtatttgtc gcgggtgtct ggctgatgcg
     1201 cgctgctgga tgtgtggtga atgattatgc tgaccgaaag tttgatggtc atgttaagcg
     1261 cacggcgaac cgaccacttc ccagcggcgc ggtaacagag aaagaggcgc gcgcgctgtt
     1321 tgtagtgctg gtactgattt cgtttttact ggtactgacg ctgaatacga tgaccattct
     1381 gttgtcgatt gccgctctgg cgttggcgtg ggtgtacccg tttatgaagc ggtataccca
     1441 tctcccgcaa gtggtgctgg gcgcggcgtt tggctggtcg attccaatgg cttttgccgc
     1501 tgtgagtgag tcggtgccac tgagttgctg gttaatgttc ctcgccaata ttctctgggc
     1561 ggtggcttac gacacgcagt atgcgatggt tgaccgcgat gatgatgtga agattggcat
     1621 taaatccacg gcaatcctgt tcggccaata cgataaattg attattggta ttttgcagat
     1681 tggcgtactg gcactgatgg cgatcatcgg tgagttaaat ggcttaggct ggggatatta
     1741 ctggtcaatt gtggtggctg gcgcgctgtt tgtttatcaa caaaaactga ttgccaaccg
     1801 cgagcgtgaa gcctgcttta aagcatttat gaataataac tatgttggtc tggtactgtt
     1861 tttaggactg gcaatgagtt actggcattt ctgatgatgt aaaaaagccg gatgatcatc
     1921 cggctttctt ctgggttgcc ttatgcgcgg cgcttctcag gcctacacaa cacatcgcaa
     1981 tttattgaat ttgcagatta tggaaggccg gataaggcgt tttcgccgca tccggcaatt
     2041 ctctctgatt acccttcgcc ctgcgtcgca ctctcaatcg tcaaacgcac gtctgatgta
     2101 atcaactccg ccagcaactg ataaaccttc atcgtttctg ccggttcggc gtcgccgcta
     2161 tcgctgatat acccttcatc acgcagcgtc agcaccagag aactgaacac cgccttgtcg
     2221 aagaactccg gcgcgttgat gccgtgcagc acggagagac gttgcgcgac ggtgcggctc
     2281 tctttctcca gcgtaccgcg gttgatcgac gggttggcac tcaacaacca gaaggtgatg
     2341 gcataacgtt gcagcgtttc gcgcgcgcct gcggccagca gctgtagggt gcgagaatgc
     2401 gccgggttga tatgcaactc atcatcttgc agggtaatca gcccctgacg ttgcatctca
     2461 tttgccagcg catcaataac gtccggcaac tcgtcgcgat cccagcgcag gaacagctcc
     2521 gctttcagca ttgggtaaag cacattgacg tgctccatca atacgtcgcg ggagatgtgg
     2581 cgatgctggg tgacaattgc cgccatcagc gaaggcagca ccaacatatg cgcaatgttg
     2641 ttgcgatagt aggtcatcag caccgcttgc tcgcgtggca gaatgatgat gtcgccgata
     2701 gtgtctttct cgacttcaaa cttgttcatt tgcagcgcgt gatcgataag ctcgctggcg
     2761 ctggctgaag gaacggtaga gtccgtggag taaggtacgt tgcgcatcag atccaggtag
     2821 cagttgagtt gctcggttaa ctgctcgcgg gtgagtgagc gctgacgtga tgccagaagc
     2881 gcagtacaac acaggttcat ggcgtttgcc gcgcctgcgt tgttaatgcg taccatcaga
     2941 tcggcagcaa tattattgac cgtcggcgtt aaccatgccg gacgcaccgc ttcgatggga
     3001 tcgatagatt cacgccagtc tggtacgtgc tggttaaggt aggtcatcaa cggcattggt
     3061 tcaccgaagt tgacgtaacc ctgaccgaga ttacgcagct tgcttaaacc gcgcagcatc
     3121 tgcggcaggc tctctttctc tttcgtcgcg ccgcgcagtt ctttggcgta agtacccact
     3181 tccatgacgt gctcataacc gatatagatc ggaatcagcg taatcggacg cgtgccgcca
     3241 cgcagcatcg cctgaatggt catcgacagc gtaccggttt tcggatccag caaacgcccc
     3301 gtgcgggaac gaccgccttc cacgaagtac tcgacggaat aaccacggct gaacagttcg
     3361 ccgagatact cacggaaaac ggtggaataa agtttattgc ctttaaaagt acggcgaata
     3421 aagaacgccc ccagacggcg gaaaatcggc ccggccggcc agaagttcag gttgatcccg
     3481 gcggcgatat gcggcggcac cagcccctgg tggtacagca cgtaagaaag tagcaggtag
     3541 tccatgtgac tgcggtggca aggcacatac accagctcat gaccgtcgtg ggccagctgg
     3601 cgaacgcgct cggcgttatg gacgttgatg ccctgataaa gtcggttcca ggtgaagccc
     3661 agaatgcggt cagtcaggcg gatcatctcg taagagaaat tcgccgcaat ctcttccatc
     3721 agcgcaatcg cgttctgctg cgctttttca tgggagattt ttttgctgcg cgcttcatct
     3781 tctaccgctt tggcaatggc gcgggaggcg agcagcttat taaacagatc ctgacgagca
     3841 ggcagacgtg ggcccacagc agccagacgt tgacgggcaa agtgcatacg cgccacgcgc
     3901 gccagtttct gagcgatagt tttatctgtg ccgtgttcat cggccatgcg gcgcagagaa
     3961 actgacggcg agaaacgcac aaaactgtcg cgaccgagcc acagtacggc gaaaaatttt
     4021 tgtacgccgt taagcatacg cagcggcggg tttacttcgc ctttttcacg ccccggcgcg
     4081 cgaccaaaca tcaccgatac tggcaccatc tgcacatcca gatttgggtt gctgcggtgc
     4141 aaatcaagat agtcgtggaa cagcttaata gactcttctt tcggcgtgta ataggtgaac
     4201 acacgcggcc cgccgtgaat gaacacatag cgcggcagta gcgtgccgtc gatttccagc
     4261 ggctctaacg ggtcaggcaa gtcatgagcc agacactggg cgcgcaacgt cagcaaatct
     4321 gctttcgagt tgtacggtaa aacgtacata attggacgag aggtatccag ccccagttcc
     4381 ggggcaggat ctgccggaat agacttgctt tttaccagga tgcttaatgg taaattcagt
     4441 aatttgtagt aaattcgtgg ccagccggac ataaacgatg taaagcctct ggttaataat
     4501 gcaaatgcgc ggcaaggata gcagaaagtc atgggaaatt ctgtggtatc cgctcatgtt
     4561 tcgcgcggcg ttacgcaaac ccgaatcatc ggatttaacg gtacactgat attgacgctc
     4621 ataatgtaaa aaggttcttt caatggccaa taataccact ggattcaccc gaattatcaa
     4681 agctgctggc tattcctgga aaggtttacg cgctgcatgg atcaacgaag cggcattccg
     4741 tcaggaaggc gtagcggtat tgttggcggt ggtcatcgcc tgctggctgg atgtggacgc
     4801 aattacccgc gtgctgctta tcagctccgt gatgctggtg atgattgtgg aaatcctcaa
     4861 tagcgccatc gaagcagtgg ttgaccgaat tggctctgaa taccatgagc tttccggacg
     4921 cgcaaaagat atgggatccg ccgcggtgct gattgccatt atcgtcgccg tgattacctg
     4981 gtgcatcctg ttatggtcgc attttggata acccttccag atttcgataa atctctggtt
     5041 tattgtgcag tttatggttc caaaatcgcc ttttgctgta tatactcaca gcataactgt
     5101 atatacaccc agggggcgga atgaaagcgt taacggccag gcaacaagag gtgtttgatc
     5161 tcatccgtga tcacatcagc cagacaggta tgccgccgac gcgtgcggaa atcgcgcagc
     5221 gtttggggtt ccgttcccca aacgcggctg aagaacatct gaaggcgctg gcacgcaaag
     5281 gcgttattga aattgtttcc ggcgcatcac gcgggattcg tctgttgcag gaagaggaag
     5341 aagggttgcc gctggtaggt cgtgtggctg ccggtgaacc gcttctggcg caacagcata
     5401 ttgaaggtca ttatcaggtc gatccttcct tgttcaagcc gaatgctgat ttcctgctgc
     5461 gcgtcagcgg gatgtcgatg aaagatatcg gcattatgga tggtgacttg ctggcagtgc
     5521 ataaaactca ggatgtacgt aacggtcagg tcgttgtcgc acgtattgat gacgaagtta
     5581 ccgttaagcg actgaaaaaa cagggcaata aagtcgaact gttgccagaa aatagcgagt
     5641 ttaaaccaat tgtcgttgac cttcgtcagc agagcttcac cattgaaggg ctggcggttg
     5701 gggttattcg caacggcgac tggctgtaac atatctctga gaccgcgatg ccgccaggcg
     5761 tcgcggtttg tttttcatct ctcttcatca ggcttgtctg catggcattc ctcacttcat
     5821 ctgataaagc actctggcat ctcgccttac ccatgatttt ctccaatatc accgttccgt
     5881 tgctgggact ggtcgatacg gcggtaattg gtcatcttga tagcccggtt tatttgggcg
     5941 gcgtggcggt tggtgcaacg gcgaccagct ttctctttat gctgttgctg tttttacgca
     6001 tgagcaccac cgggctgact gcgcaggctt atggtgccaa aaatcctcag gcattagccc
     6061 gtgcgctggt gcaaccgttg ctgttggcgt tgggggctgg ggcgttaatt gcgctgctgc
     6121 gtacaccgat tatcgatctg gcgctgcata ttgttggcgg tagtgaagcg gtcctggaac
     6181 aggcgcggcg ctttcttgaa atccgctggt taagcgctcc ggcgtcgctg gcgaatctgg
     6241 tattactcgg ttggttactc ggcgtgcaat atgcccgtgc gccagtaatt ttgttagtgg
     6301 tcggcaatat cctcaacatt gtgctggatg tctggctggt gatggggctg catatgaacg
     6361 tgcagggcgc ggcgctggcg acggttattg cggaatatgc aacattgctg attggtctgc
     6421 taatggtgcg taaaatcctc aaactacgcg gaatttccgg cgaaatgctg aaaactgcct
     6481 ggcgaggaaa cttccgtcgc ttgctggcgc ttaaccgcga tatcatgctg cgttcgctgt
     6541 tgttgcaact ctgtttcggc gcgatcaccg tacttggtgc gcgactgggg agtgacatta
     6601 tcgctgttaa cgcggttctg atgacgctac tcacctttac cgcctatgcg ctggatggtt
     6661 ttgcctacgc ggttgaagca cattccggtc aggcatacgg tgcgcgcgac ggtagccagt
     6721 tgctggatgt ctggcgggca gcgtgccgcc agtcggggat cgtagcgcta ctgttttcgg
     6781 tggtttattt gctggcaggg gaacacatca ttgcgttact gacgtcgtta acccagattc
     6841 aggagctggc cgaccgctat cttatctggc aggtgatttt gccgttggtt ggcgtctggt
     6901 gttatctgct ggacggcatg tttataggcg caacgcgtgc cgccgaaatg cgtaacagta
     6961 tggcggtggc cgccgcaggt tttgcgctga cgctccttac gctgccgtgg ctgggtaatc
     7021 atggtgtgtg gctggcatta accgtctttc tggcgttgcg cgggctttct ctggcggcta
     7081 tctggcggcg tcactggcgc aacggtacct ggtttgccgc aacgtgacgg ttaaaaattc
     7141 tgaataaata atcctaagcc aaattgctga ctacacttaa tctcacgttc agaagaaaag
     7201 tgaacgtact ctcattcaca acctaacgat gaggtcttga ttatgaataa agatgaagcc
     7261 ggcggtaact ggaaacagtt taaaggtaaa gtgaaagagc aatggggcaa actgaccgat
     7321 gatgatatga cgatcattga aggtaaacgt gatcaactgg tcggtaaaat ccaggaacgt
     7381 tatggttatc agaaagatca ggcagaaaaa gaggtcgtgg attgggaaac ccgcaatgaa
     7441 tatcgctggt aattaatccc tcctgcccga cgtgtacaag gatgtacgcc ctcttaacgc
     7501 ggtttctttt tcacctgcac agagtgatca tgctggcact gttcaggatg acgacacgct
     7561 tccacttcca cgcatgccgc acagagccca tgtgcttcaa tcacattatg ccgcagggca
     7621 aaccccattt ttgccgccag cgtatgcata atgtcttcca cgccttctgc acactcttct
     7681 ttcaccgcgc cgcagcgatc gcaaataaac atggctgacg tatgcgtggg ctggtcgaac
     7741 agatgacaga gcacataact gttggtggat tccaccttat gcacaaaacc ttgctcaagc
     7801 agaaaatcca gcgcgcgata aaccgttggc ggcttggctt gcggttcagc ttcgcgcagc
     7861 aaatcaagca gatcataagc gctgatagcg ccatcctgca gactcatcag gcgcaacact
     7921 tccaggcgct gtggggtcag gcgcacatta cgctgcgcgc agattttttc agcctgcgct
     7981 aataactcct gcgttgtggt cttttccatc gggcacctca aagtgggggt taagaatgtc
     8041 cttactttac catgttccag gaaaaacacc gagaaccctt attgttgccg taatgttgat
     8101 tttctgtttt gtacgtaagg tgttgtgttg cttggtcgta ccattatcaa cacgataata
     8161 attaataatt catttttaaa taaattgctt caagttaact cgctatttat ttaactgggt
     8221 ttgatcgcat acctgtagtt cagaaattat gagcgatttt tgatagtgac cagatggcaa
     8281 aacacttata aggaggacac ggtacgacaa aaaaggtaga tgaatcatg
//
LOCUS       DAPHDW010000173         8193 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.176, whole genome shotgun sequence.
ACCESSION   DAPHDW010000173 DAPHDW010000000
VERSION     DAPHDW010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8193
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.176"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..552
                     /gene="ftnA"
                     /locus_tag="RH057_003989"
     CDS             55..552
                     /gene="ftnA"
                     /locus_tag="RH057_003989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HDV3130284.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(590..829)
                     /locus_tag="RH057_003990"
     CDS             complement(590..829)
                     /locus_tag="RH057_003990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HDV3130285.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGMTAGELVAFLAAKGKFIPSEDGFSTDQSKICRH"
     gene            1020..2231
                     /gene="tyrP"
                     /locus_tag="RH057_003991"
     CDS             1020..2231
                     /gene="tyrP"
                     /locus_tag="RH057_003991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HDV3130286.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(2293..2958)
                     /gene="yecA"
                     /locus_tag="RH057_003992"
     CDS             complement(2293..2958)
                     /gene="yecA"
                     /locus_tag="RH057_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HDV3130287.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(3154..3240)
                     /locus_tag="RH057_003993"
     tRNA            complement(3154..3240)
                     /locus_tag="RH057_003993"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3204..3206),aa:Leu,seq:taa)
     gene            complement(3253..3326)
                     /locus_tag="RH057_003994"
     tRNA            complement(3253..3326)
                     /locus_tag="RH057_003994"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3292..3294),aa:Cys,seq:gca)
     gene            complement(3381..3456)
                     /locus_tag="RH057_003995"
     tRNA            complement(3381..3456)
                     /locus_tag="RH057_003995"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3421..3423),aa:Gly,seq:gcc)
     gene            complement(3608..4156)
                     /gene="pgsA"
                     /locus_tag="RH057_003996"
     CDS             complement(3608..4156)
                     /gene="pgsA"
                     /locus_tag="RH057_003996"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HDV3130288.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYAGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(4213..6045)
                     /gene="uvrC"
                     /locus_tag="RH057_003997"
     CDS             complement(4213..6045)
                     /gene="uvrC"
                     /locus_tag="RH057_003997"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HDV3130289.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAARIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(6042..6698)
                     /gene="uvrY"
                     /locus_tag="RH057_003998"
     CDS             complement(6042..6698)
                     /gene="uvrY"
                     /locus_tag="RH057_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HDV3130290.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            6994..7170
                     /locus_tag="RH057_003999"
     CDS             6994..7170
                     /locus_tag="RH057_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130291.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTA
                     NFHCNMTRKINEYA"
     gene            7157..7381
                     /locus_tag="RH057_004000"
     CDS             7157..7381
                     /locus_tag="RH057_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HDV3130292.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(7448..8170)
                     /gene="sdiA"
                     /locus_tag="RH057_004001"
     CDS             complement(7448..8170)
                     /gene="sdiA"
                     /locus_tag="RH057_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
                     /protein_id="HDV3130293.1"
                     /translation="MQDTDFFSWRRTMLLRFQRMEAAEEVYHEIELQAQQLEYDYYSL
                     CVRHPVPFTRPKVAFYTNYPEAWVSYYQAKNFLAIDPVLNPENFSQGHLMWNDDLFSE
                     AQPLWEAARAHGLRRGVTQYLMLPNRALGFLSFSRCSTREIPILSDELQLKMQLLVRE
                     SLMALMRLNDEIVMTPEMNFSKREKEILKWTAEGKTSAEIAMILSISENTVNFHQKNM
                     QKKINAPNKTQVACYAAATGLI"
BASE COUNT         2034 a         2100 c         1913 g         2146 t
ORIGIN      
        1 taacaaatta tagtgacgcc acagcaacaa atataacctt tgtggagcac tatcatgctg
       61 aaaccagaaa tgattgaaaa acttaatgag cagatgaacc tggaactgta ctcttcactg
      121 ctttatcagc aaatgagcgc ctggtgcagc tatcatacct tcgaaggtgc tgccgcgttc
      181 ctgcgccgtc acgcccagga agagatgacg catatgcagc gtctgtttga ttacctgact
      241 gataccggca atttaccgcg tattaatacc gttgaatctc cgtttgctga atattcctca
      301 cttgatgaat tattccagga aacctataaa cacgaacaat taatcaccca gaaaattaac
      361 gaactggctc atgctgcaat gaccaatcag gactacccaa catttaattt cctgcaatgg
      421 tatgtttctg agcagcatga agaagagaaa ctgttcaaat cgattattga taaattaagc
      481 ctggcaggca aaagcggcga aggtctgtat tttatcgaca aagaactctc taccctcgac
      541 acacaaaact aatgctctcc ggcggcaggt ttactgccgc cgtttcaaat cagtgacggc
      601 aaatcttact ctgatcggtc gaaaaaccgt cttccgaagg tataaatttg ccttttgctg
      661 ccagaaacgc taccagctct cccgcagtca tcccttctgc cgagcaggtg tgaaaacgtg
      721 cctgttcacc aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc
      781 ctgattcaat catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc
      841 aaaaaaaagc ctagcgtagc gattaccgct tatgaagact ttgcgccagc gcaggactga
      901 atgcttttat tgtacattta tatttacacc atatgtaacg tcggtttgac gaagcagccg
      961 ttatgcctta acctgcgccg cagatatcac tcataaagat cgtcaggaca gaagaaagcg
     1021 tgaaaaacag aaccctggga agtgttttta tcgtggcggg aaccacaatt ggcgcaggca
     1081 tgctggcaat gccgctggct gcggcaggtg ttggttttag cgtcacgtta atcttgttga
     1141 ttgggctttg ggcgttgatg tgctacacgg cgctattact gctggaggtg taccagcatg
     1201 ttccggcaga taccggtctt ggcacgctgg caaaacgcta tctgggacgc tacggtcaat
     1261 ggctgacggg cttcagtatg atgttcttaa tgtatgctct gactgcggca tacatcagcg
     1321 gtgccggtga attgttggcc tccagcatca gcgactggac aggtatttct atgtcggcaa
     1381 ccgctggcgt gctgttgttc acttttgttg ccggtggcgt ggtttgtgtc ggaacttcac
     1441 tggtcgattt atttaaccgt tttctgttca gcgccaagat tatttttctg gtggtaatgc
     1501 tggtactact gctgccgcat attcacaaag tgaatctttt aaccctgccg ttgcaacagg
     1561 ggctggctct gtctgcaatc ccggtgattt ttacgtcgtt tggttttcac ggtagcgtgc
     1621 cgagtattgt cagctatatg gatggcaaca ttcgtaagct acgctgggtg tttataatcg
     1681 gtagtgcgat ccccctggtg gcatatattt tctggcaggt ggcgacgctt ggcagcattg
     1741 attcaacaac ctttatggga ttgctggcta atcatgctgg attaaacggg ctgttacagg
     1801 cgttacgcga aatggtggcc tctccgcatg ttgagctggc agtgcattta tttgctgatt
     1861 tagccctcgc cacgtcattt ctcggcgttg cgttaggctt atttgattat ctggctgatt
     1921 tatttcagcg ttcaaatacc gttggtggac ggttgcaaac tggtgcaatt acgtttctgc
     1981 cgccgttggc gtttgcactg ttttatccac gaggatttgt gatggcgctg ggttacgccg
     2041 gtgtggcgct ggcggtactg gcattgatta tcccttcgct gttgacctgg caaagcagaa
     2101 agcacaatcc tcaggcgggt taccgggtca aaggtggtcg tccggcgctg gtggtggtgt
     2161 ttctctgtgg tattgctgtg attggcgtgc aatttttgat tgcggcaggg ttgttaccag
     2221 aagtggggtg atcagatagc ctcaaattcc ttattgggtg ccagaattaa cgctgacacc
     2281 caatttggcc tcttaatgca ggcagcactg cttaaatttc ttaccactac cgcacgggca
     2341 aggatcgtta cgccccggtt tctcttctgc tttgatcggt tgctgaacag ctttttcctg
     2401 cggatgcgcc atccagtacg catgtagatc aagcgccgcc agtcgaatgg catctacgct
     2461 ctcttcaaac gcttctggcg acatcttttc tacccgctcg aagttttcct cagtaccgtg
     2521 cagcgcaatc gcctccagcg ctggttttaa cgaatcgggc aacgttgacc agtcagaaag
     2581 tgccacgccc cgcatatagc caaagcacca ctcctcaaca atcgtcagct cgctgccatc
     2641 aacttctcgc aagccgaata acggctcaaa ctgctccggg aattcgttca gacgctctgc
     2701 ggtatcggcc atatgttgaa aagccagatt cataaagcgc gtcatctctt tctctgacgc
     2761 ccagcgcggc acatagtcag ccccacccca cacggcaacc agccactgtt ccggttcaat
     2821 ctcttgcgga gaactcaaca ccgccgtcaa taaaccgtcc agctccgcca catcaaggat
     2881 ggcgtggtca gtgttgtatt tggtcagaat atcgtccagc cattccaact cactttcgtt
     2941 taacggtccc gttttcatac gcttttcctt gtggatctca actcgccagc acctatctta
     3001 catgccggtc cgtatcagag atactttttg agtggctttg ctggtgatta aaaattaggg
     3061 agggtgtaac gacaagttgc aggcacaaaa aaaccacccg aaggtggttt cacgacactg
     3121 cttattgctt tgattttatt cttatctttc ccatggtacc cggagcggga cttgaacccg
     3181 cacagcgcga acgccgaggg attttaaatc ccttgtgtct accgattcca ccatccgggc
     3241 tcgggaagaa agtggaggcg cgttccggag tcgaaccgga ctagacggat ttgcaatccg
     3301 ctacataacc gctttgttaa cgcgccaaat tcttcaggcc tttcagccag acatccgctt
     3361 gacgccgatg tcttttaaac tggagcggga aacgagactc gaactcgcga ccccgacctt
     3421 ggcaaggtcg tgctctacca actgagctat tcccgcattc atcaagcaat cagttaatca
     3481 cttgatttta ttatcgtctg gcaatcagtg ccgccgttcg atgcgttgca ttctacttac
     3541 ctggcgcgat gagtcaacga tatttttcac cacttttgat cgtttgctga aaattacgcc
     3601 gaaacgatca ctgatcaagc aaatctgcac gcgcagcgct caaatattgc aacattgacc
     3661 acagagtcag taccgcagcc acaaagaaaa gtgcaatacc ggcgtactca acccaaatgt
     3721 tcggacgcca cagcagccat gccaacgcca ccatctgggc agtggttttc actttcccaa
     3781 tccaggagac ggccacgcta ctgcgtttac ccaactccgc catccattcg cgtagcgcag
     3841 aaataataat ttcacgggcg atcatcgttg ccgccggtaa tgtcacccac cagctgtgat
     3901 aatgctcggt taccagcacc atggcgatag ccacgagaac tttatctgcc acagggtcaa
     3961 ggaaagcacc aaaccgggta ctctggttcc agcggcgtgc cagaaaacca tcgaaccagt
     4021 cagtcaccgc cgcgacgcag aaaatgagcg cggcggcaaa cggcgaccag gtgacaggca
     4081 gataaaagac caatacaaag aatgggataa ggatgacacg gaacagtgta agcaacgtag
     4141 ggatattaaa ttgcataatg acgggtaact atctgttgtc agtaagatta cccctatgtt
     4201 gctacagaga catcaatgtt tcaacgacca gaagatcttt tctgccagac cttgcgaaat
     4261 acccggcact tttgcaattt cctcgacgct ggcgttacgt aaaccttgca aaccgcccat
     4321 atatttcaac aacatttgcc gacgttttgg cccgacgcct tcaatggttt ccagggaact
     4381 ggtatttttg accttcgccc tttttttacg gtgcccgcca atcgcgtgat catgtgattc
     4441 atcgcgaata tgctggataa catgcagcgc gggtgaatct ggcggcaaac taaatccctc
     4501 accttccggc tcaaagaaca gtgtttccag cccagcctta cgatctgctc ctttggcaac
     4561 gccaagtagc agtggatgat ttttatccca tgagacatcc agttcggcga agacattttt
     4621 cgcctgcgca agctggcctt tgccgccgtc gataaggatc acatccggga tcttactgtc
     4681 gtcaatggct ttaccataac gccgacgcag cacctgattc atcgccgcat aatcatcgcc
     4741 cggcgtgata cctgtaatgt tatagcgccg atactccgca cgcagcgggc cgttagcatc
     4801 aaacaccaca caggaagcga cggtttgttc gcccatggta tggctgatgt caaagcactc
     4861 catccgcttc acttccggca atttcaacac gctggcaagc gcggtcagtc gctggtgaac
     4921 ggtagattgc tgcgaaagtt tgctggttaa ggccgtcgcc gcattggtgc gcgcgagttt
     4981 cagataacgc gccctatcgc cgcgaggttt ggtttgaaca ttaatcttgc gtcccgccag
     5041 ttctgaaagg gaatcggcga gcagcgtttt atcgctaaga ttaaaatcga gcaggatctc
     5101 acccggtaag gtgcgcatct ggctgccttg taaatagaac tggcctacga aggtttctac
     5161 cacctcgctc agttccgtac cgccaggcac tttcgggaaa tagctgcggc tgccgagcac
     5221 tttgccctga cgaatgaaca atacgtggac acaagccatg cccgcatcga acgccacacc
     5281 aataacgtcg aggtcgtcgc cggtattgga aacgaattgt ttttcggtga cgcgtcgcac
     5341 cgcctgaatt tggtcgcgaa tacgtgcagc ttcttcaaac tccagattct ggctggcagt
     5401 ttccatacga ctaatgagtt gcgtaagcac ctgatcatct ttgccagaca aaaacaggcg
     5461 cacatactcg acctgctgag cgtattcttc ttcactcacc agtccttcaa cgcacggtcc
     5521 cagacagcgc cctatctggt attgcagaca cggacgcgag cgattgcgat aaacactatt
     5581 ttcgcactgg cgaatgggga aaatcttttg cagtagcgcc agtgtttcac gtacggcata
     5641 gccattcggg aacgggccga aatattcacc tttggcatgc ttcgcaccac gatgcatcgc
     5701 cagacgcggg tgggtatcac cactcaggaa gataaaagga tatgatttat catcgcgtag
     5761 caaaacgttg taacgcggct gatagagttt gatgtagttg tgttccagca acagcgcttc
     5821 ggtttctgtg tgagtaaccg ttacatcaat ttgctggatc tgggcgacca gcgcttcggt
     5881 tttgcgcgaa gcgaggttgc tacggaaata gctggaaagc cgttttttca ggtctttcgc
     5941 tttgccgaca tagataaccg taccaccagc atcgtacatg cgataaacgc ctggctggct
     6001 ggttacggtt tttaaaaacg cttttgcgtc aaactgatca ctcactgact tgataatgtc
     6061 tccgcattac acagaccatg gcgaattgcc aggtgagtca gctcaacatc gccatgaatg
     6121 tttagtttac tgaacatacg atagcggtag ctgttcaccg ttttcggact gagattgagc
     6181 tgttctgaga tctcattgac cttctggccc ttggtgatca tcagcataat ctgcaattca
     6241 cgttcagaca aactggcaaa tgggctttct gttttttctg gttcgatctg gcttaacgcc
     6301 atttgttgag cgatgtcaga agcaatgtaa cgctgccctg aatagacaga acgaatcgca
     6361 ctcacaactt cctgcggagc cgcgcctttg ctgaggtagc ccgcagcacc ggcctgcatg
     6421 actttcgctg gtaaagggtt ttctgtatgg acggtaagca tgatgatttt gacatcagct
     6481 gtggaacgcg cgattttacg cgtcgcctca agaccgccaa tgcccggcat actcatgtcc
     6541 attagcacca cgtcaacggc atttgtccgg caccacttaa cggcgtcttc accgcacgat
     6601 gcctcaccga cgacttttat accctttata tcttccagaa tgcgtcgtat ccctgcgcgc
     6661 accagttcgt ggtcatcaac aagtagaacg ttgatcaaag gaatatctcc agaaataggg
     6721 ataacgctac tgatagttag tcattcgtat attagcggtt tttattgcaa ctttgaaacg
     6781 ttaaaaatgg tgggtattcg atttttctct cgtttttgtc aattcgattg tccacagtta
     6841 tggtcacgcc cgtcaccaac ggcttaccgg atcgtttcac agtaagccct acgatgaaat
     6901 gtgacaaaaa ttgactttat tcagcaaaaa tgaaaatcag cccgctgaat atgtaacatt
     6961 aattaacccg aacaatacgg gcaaaaaaca ttcatgataa aaatatttat tggtcattat
     7021 attaacgtat tttatagcac tgccgatatc acgctcaaaa aacaaccact gctattttta
     7081 gcaaagctta tggtatactc cgccgcctta acatttttca ccgcaaattt tcattgcaac
     7141 atgacgagga aaataaatga gtacgcctga tttttctact gccgagaata atcaagaact
     7201 ggcaaatgaa gtctcctgcc tgaaagcgat gctgacgctg atgctgcagg cgatgggaca
     7261 agctgacgcg ggccgcgtga tgttaaagat ggaaaaacag cttgcgctga tcgaagacga
     7321 aacccaggct gcagtatttt ccaaaacggt taagcaaatt aaacaagcct accgtcagta
     7381 atataaaacc ggctgagagc gtgcctttca gccggttttt gcatctggca cgcagaaaga
     7441 aaagcgatca aattaagcca gtagcggccg cgtaacaggc cacctgggtc ttatttggtg
     7501 cattgatttt tttctgcatg tttttctgat ggaaattgac cgtattctca gagattgaca
     7561 aaatcatcgc tatctctgct gatgtcttcc cttccgccgt ccatttcaga atttcttttt
     7621 cgcgcttgct gaaattcatc tctggcgtca tcactatttc atcatttaaa cgcatcagag
     7681 ccatcagact ttcgcgcacc agtaactgca tttttaattg cagttcatca ctaagaatgg
     7741 gtatttcgcg cgtgctgcaa cgggaaaagg acaaaaagcc cagcgcccga tttggcagca
     7801 ttaaatactg agtgacaccg cggcgtaaac catgtgcgcg cgcggcttcc cataacggct
     7861 gtgcttcgct gaataagtca tcattccaca ttaaatggcc ctgactaaag ttttcagggt
     7921 tcagcaccgg atcaattgcg agaaagtttt ttgcctgata ataactaacc cacgcctcag
     7981 ggtaattggt gtagaaagcc actttaggtc gcgtgaatgg taccgggtgg cgaacacata
     8041 acgaatagta atcgtactcc agctgctgag cctgaagctc tatttcatgg tagacctctt
     8101 ctgcggcctc catcctctga aaacgcaaca gcatcgtgcg acgccagctg aaaaaatccg
     8161 tatcctgcat agtaaaccgc aacgcccctg aga
//
LOCUS       DAPHDW010000174         8082 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.177, whole genome shotgun sequence.
ACCESSION   DAPHDW010000174 DAPHDW010000000
VERSION     DAPHDW010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.177"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1062
                     /locus_tag="RH057_004002"
     CDS             <1..1062
                     /locus_tag="RH057_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HDV3130294.1"
                     /translation="YKPHQDDYSSAEKFLTHFNKKCKNQTLALISSRPEGRCVAACGD
                     FGLVMKAYFDKMESNGISVMAAILLVDNHALTARLRIKNTTEGCTHYVVSVYDPNVTN
                     DKIRIMSESKEDIKHYSLMDFMNVDYSLLKWSNDHVINQSVAIIPALPKEQLLMLKGS
                     VDEIPPPLSPATMNLLMAIGQNHQLTQLMIQLQKMPELHRTEMLTAYNSGHMNVINTI
                     FNALPTLFNTFKFDKKNMKPLLLANNSNEYPGLFSAIQHKQQNVVETVYLALSDHARL
                     FGFTAEDIMDFWQHKAPQKYSAFELAFELGHRVIAELILNTLNKMAESFGFTDNPRYI
                     AEKNYMEALLKKASPHTVR"
     gene            complement(1031..2767)
                     /gene="aceK"
                     /locus_tag="RH057_004003"
     CDS             complement(1031..2767)
                     /gene="aceK"
                     /locus_tag="RH057_004003"
                     /EC_number="2.7.11.5"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
                     /protein_id="HDV3130295.1"
                     /translation="MPRGLELLIAQTILQGFDAQYGRFLEVTSGAQQRFEQADWHAVQ
                     QAMKNRIHLYDHHVGLVVEQLRCITNGQSTDAAFLLRVKEHYTRLLPDYPRFEIAESF
                     FNSVYCRLFDHRSLTPERLFIFSSQPERRFRTIPRPLAKDFHPDHGWESLLMRVISDL
                     PLRLRWQNKSRDIHYIIRHLTETLGTDNLAESHLQVANELFYRNKAAWLVGKLITPSG
                     TLPFLLPIHQTDDGELFIDTCLTTTAEASIVFGFARSYFMVYAPLPAALVEWLREILP
                     GKTTAELYMAIGCQKHAKTESYREYLVYLQGCNEQFIEAPGIRGMVMLVFTLPGFDRV
                     FKVIKDKFAPQKEMSAAHVRACYQLVKEHDRVGRMADTQEFENFVLEKRHISPALMEL
                     LLQEAAEKITDLGEQIVIRHLYIERRMVPLNIWLEQVEGQQLRDAIEEYGNAIRQLAA
                     ANIFPGDMLFKNFGVTRHGRVVFYDYDEICYMTEVNFRDIPPPRYPEDELASEPWYSV
                     SPGDVFPEEFRHWLCADPRIGPLFEEMHADLFRADYWRALQNRIREGHVEDVYAYRRR
                     QRFSVRYGEMLF"
     gene            complement(3048..4352)
                     /gene="aceA"
                     /locus_tag="RH057_004004"
     CDS             complement(3048..4352)
                     /gene="aceA"
                     /locus_tag="RH057_004004"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HDV3130296.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINSLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSAGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPELELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTAMTGSTEESQF"
     gene            complement(4382..5983)
                     /gene="aceB"
                     /locus_tag="RH057_004005"
     CDS             complement(4382..5983)
                     /gene="aceB"
                     /locus_tag="RH057_004005"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HDV3130297.1"
                     /translation="MTEQATTTDELAFIRPYGEQEKQILTAEAVEFLTELVTHFTPQR
                     NKLLAARIQQQQDIDNGTLPDFISETASIRDTDWKIRGIPADLQDRRVEITGPVERKM
                     VINALNANVKVFMADFEDSLAPDWNKVIDGQINLRDAVNGTISYTNEAGKIYQLKPNP
                     AVLICRVRGLHLPEKHVTWRGEAIPGSLFDFALYFFHNYQALLAKGSGPYFYLPKTQS
                     WQEAAWWSEVFSYAEDRFNLPRGTIKATLLIETLPAVFQMDEILHALRDHIVGLNCGR
                     WDYIFSYIKTLKNYPDRVLPDRQAVTMDKPFLNAYSRLLIKTCHKRGAFAMGGMAAFI
                     PSKDEERNNQVLNKVKADKALEANNGHDGTWIAHPGLADTAMAVFNDILGSRKNQLEV
                     MREQDVPITADQLLAPCDGERTEEGMRANIRVAVQYIEAWISGNGCVPIYGLMEDAAT
                     AEISRTSIWQWIHHQKTLSNGKPVTKALFRQMLGEEMKVIASELGEERFSQGRFDDAA
                     RLMEQITTSDELIDFLTLPGYRLLA"
     gene            complement(6252..7181)
                     /gene="metA"
                     /locus_tag="RH057_004006"
     CDS             complement(6252..7181)
                     /gene="metA"
                     /locus_tag="RH057_004006"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HDV3130298.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYFRDVEAGLGPEVPYNYFPHNDPQNTPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            7338..7781
                     /locus_tag="RH057_004007"
     CDS             7338..7781
                     /locus_tag="RH057_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HDV3130299.1"
                     /translation="MVISIRRSQHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNERDQPVGFMLLSGQHMDALFIDPDVRGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
     gene            complement(7899..8014)
                     /gene="rrf"
                     /locus_tag="RH057_004008"
     rRNA            complement(7899..8014)
                     /gene="rrf"
                     /locus_tag="RH057_004008"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         2003 a         2038 c         2035 g         2006 t
ORIGIN      
        1 tacaaacccc atcaagatga ttactcttca gctgaaaaat ttctcactca cttcaacaaa
       61 aaatgcaaaa accagacttt ggcgttgatt tccagccgtc ctgaggggcg ttgtgttgct
      121 gcctgtggtg atttcgggct agttatgaag gcatatttcg acaagatgga atcaaatggc
      181 atcagtgtta tggcagccat attattggtg gataaccatg ctttgacggc gcggctaaga
      241 ataaagaaca caactgaagg atgtacccat tacgtggttt cggtttatga tcctaatgta
      301 actaacgata aaataagaat tatgagcgaa agcaaagagg atattaaaca ctattctctg
      361 atggatttta tgaatgtaga ttatagcctc ctgaaatggt caaatgatca tgttattaac
      421 caatctgttg caataattcc agcacttccg aaagaacagc tattgatgtt aaaaggatct
      481 gtggatgaaa taccccctcc attatcacca gcaacgatga atttgctaat ggcaattggt
      541 cagaatcacc aacttacgca actgatgatt cagctccaga aaatgccaga actacataga
      601 acagaaatgt tgactgccta taatagtgga catatgaacg ttattaatac tatttttaac
      661 gcattaccca ctctgtttaa tacgtttaaa ttcgataaaa aaaatatgaa gcccctcctc
      721 ctggcaaata attctaatga atatcccggt ttgttttcag cgatacagca taaacaacaa
      781 aacgttgtag agacggttta tcttgcttta tctgaccatg cacgcctgtt tggatttacc
      841 gctgaagata ttatggattt ttggcaacac aaagccccac aaaaatactc tgcctttgag
      901 ttggcttttg aattgggtca ccgggttatt gctgaattaa tccttaatac attaaataag
      961 atggctgaaa gctttggctt tacggataac cctcgataca ttgcggagaa aaattatatg
     1021 gaagctttac tcaaaaaagc atctccccat accgtacgct aaatctttgc ctgcgccgat
     1081 acgcataaac atcttccaca tgcccttcac gtatgcggtt ttgtagtgcg cgccagtaat
     1141 cagcgcggaa caggtcggcg tgcatctctt caaacagcgg accaatacgc gggtcggcgc
     1201 atagccagtg gcgaaactct tccgggaaaa catcgcccgg cgagacgctg taccacggtt
     1261 cgctggcaag ttcgtcttcc ggatagcgcg gcggcgggat gtcgcggaaa ttcacttccg
     1321 tcatgtagca aatttcatcg taatcataaa aaaccacacg cccgtgacgg gtgacaccga
     1381 agtttttaaa cagcatgtcg ccaggaaaaa tgttagcagc ggcaagctgg cgaatagcgt
     1441 taccgtattc ttcaatggcg tcgcgcaact gctgaccttc cacttgttcc agccagatat
     1501 tgagcggcac catccgccgc tcgatataga gatggcgaat tacaatctgc tcaccaagat
     1561 cggtgatttt ttccgctgct tcctggagca gtaattccat taatgccggg gaaatatgcc
     1621 gcttctccag cacaaagttt tcaaactcct gggtgtccgc cattcggccc acacggtcgt
     1681 gctctttcac cagttgatag caggcacgaa cgtgagcggc agacatctct ttctgcggcg
     1741 cgaacttgtc tttgatgact ttgaataccc gatcaaagcc cggcagcgta aataccaaca
     1801 tcaccattcc acgaataccc ggcgcttcaa tgaactgctc attacagccc tgtagataaa
     1861 cgagatattc gcggtagctt tcggttttgg cgtgcttctg gcagccgata gccatataca
     1921 attcagcagt ggttttacct ggcagaattt cccgtagcca ctcgaccagc gctgcgggca
     1981 gcggtgcata aaccataaaa taagaacgcg caaagccaaa aacaatgctc gcttcggcgg
     2041 tcgtcgtcag gcaggtatca ataaataact cgccgtcgtc cgtctggtgg atcggcagta
     2101 agaagggcaa cgtgccggaa ggcgtgatca gtttgcccac cagccaggcg gctttattgc
     2161 ggtaaaacag ttcgttcgcc acctgtaaat gactttccgc gaggttgtct gtccccagcg
     2221 tttccgtcag atggcgaatg atgtaatgaa tgtcacggct tttattctgc cagcgcaggc
     2281 gcagcggcag gtcgctgata acgcgcatca gtagagattc ccagccgtga tcggggtgaa
     2341 agtctttcgc cagcgggcgg ggaatggtac gaaagcggcg ctctggctga gaactaaaga
     2401 taaaaagccg ctcgggagta agcgagcggt ggtcaaataa ccgacagtac acggagttaa
     2461 aaaagctctc cgcaatctcg aagcgcgggt aatccggcaa cagccgcgtg taatgctctt
     2521 taacgcgcag taaaaatgcc gcgtccgtac tttggccgtt agtaatgcag cgcagttgct
     2581 ccacgaccag accaacgtga tgatcgtaaa gatggatacg gtttttcatc gcctgctgga
     2641 cagcatgcca gtcggcctgt tcgaaacgct gctgcgcccc ggaagtcact tcgaggaatc
     2701 gaccatactg agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat tccaggccac
     2761 gcggcattta gcgccctcat caggagcaga gaattgccgg atgcggcgta aacgccttat
     2821 ccagcctacg ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc gcatcaggca
     2881 tcgtgcaccg attgccggat gcggcgtaaa tgccttatcc ggcctacgtt cggcaatggc
     2941 tgtaggcatg ataagacgcg caagcgtcgc atcaggcatc gtgcaccgat tgccggatgc
     3001 ggcgtgaacg ccttatccgg cctacagtca gcaacggttg ttgttgctta gaactgcgat
     3061 tcttcagtgg agccggtcat cgcggtgact gaagacgtgc cgccctgaat aatagtcgtc
     3121 actttatcga agtaacctgt acccacttcc tgctggtgag atacgaaggt atagccatct
     3181 ttcgcggcgg caaattccgg ctgctgcact ttctcaacgt agtgcttcat accctcgccc
     3241 tgggcatagg cgtttgccag gtcaaacatg ttgaaccaca tgctgtggat acctgccagg
     3301 gtgatgaact ggaacttgta gcccatatcc gacagctgct gctggaagct ggcaatagtt
     3361 ttgtcgtcga ggtttttctg ccagttgaac gacggcgagc agttataagc cagcagtttg
     3421 cccggatatt tcgcgtggat agcttgtgca aagcgacgcg ccagttccag ctccggcgtg
     3481 gaggtttcac accagaccag gtcagcatat ggcgcatacg ccaggccacg gctgatcgct
     3541 tgctcaatgc ccgcatgagt acggaagaag ccttcactgg tacgctcgcc agtaataaat
     3601 tcactgtcat acgggtcgca atcggaggta atcagatccg ccgcatcggc atcggtacga
     3661 gcaaccagca gggttggaac gcccgtcacg tcagctgcaa gacgcgccgc gaccagtttc
     3721 tgaatagctt cctgagttgg caccaaaact ttgccgccca tgtgaccgca tttcttcact
     3781 gacgccagct gatcttcgaa gtgaactgcc gctgcaccgg cttcaatcat cgctttcatc
     3841 agttcaaagg cattcaggac accgccaaaa ccggcttccg catcggcaac gatcggcagg
     3901 aagtaatcga catagcgcgg atcgcccggc tcaatgcctg cggaccattg gatctgatcg
     3961 gcacgacgga aggtgttgtt gatccgctcc accacagctg gcaccgagtt tgccggatag
     4021 agcgactgat ccggatacat gctggccgcc aggttagcgt ccgccgctac ctgccatccc
     4081 gacagataga ctgcttcaat acccgctttc gcctgttgca gcgcctgacc gccagtcagt
     4141 gcaccgaggc tgttgatgta gccttttttc gactcgccat gcagcagacg ccacattttc
     4201 gccgcgccca gttgcgccag ggtgcattca ggattgactg aaccgcgtaa tttcaccaca
     4261 tcttccgcgc tgtatgggcg agtaatgcct tcccaacgcg gttgagtcca ctctttctgt
     4321 aattcttcaa tttgttgtgt acgggttttc atgtgcagat gctccatagt tatgtggtgg
     4381 tttacgctaa caggcggtag cctggcaggg tcaggaaatc aattaactca tcggaagtgg
     4441 tgatctgttc catcaagcgt gcggcatcgt caaaacgccc ctgggagaaa cgttcttcgc
     4501 ccagttcgct ggcaatgact ttcatctctt cacccagcat ctggcggaac aaggctttgg
     4561 tcaccggttt gccattgctc aacgtttttt gatgatggat ccactgccag attgaggtac
     4621 gggaaatttc agccgtcgcc gcatcttcca tcaggccata aatcggcacg cagccgttgc
     4681 cggagatcca tgcttcgatg tactgcactg ccacgcgaat gttggcgcgc ataccttctt
     4741 cggtgcgttc accatcacaa ggtgccagca gctgatcggc agtaatcggc acgtcttgtt
     4801 cgcgcatcac ttcaagctga tttttacggg agccgagaat gtcgttgaat accgccattg
     4861 ccgtatccgc aaggcctggg tgagcaatcc atgtgccatc gtgaccgtta ttggcttcca
     4921 gcgctttatc cgcttttact ttgttgagca cctggttatt gcgctcttca tctttgctcg
     4981 gaataaacgc tgccatgccg cccatcgcaa aagcaccgcg tttatggcag gttttaatca
     5041 acaggcgtga gtaagcattc aggaatggtt tatccatcgt cactgcctgt ctgtctggca
     5101 ggacgcgatc gggatagttt ttcaacgttt tgatatagct gaagatgtaa tcccaacgac
     5161 cgcagttcag accaacaata tggtcacgca gcgcgtgaag gatttcatcc atctggaaca
     5221 cggcgggcag cgtttcaatc agcaacgtcg ccttgatggt gccgcgcggc agattaaagc
     5281 gatcttctgc atagctgaag acttcgctcc accaggccgc ttcctgccag gactgggttt
     5341 tcggcagata gaaataggga ccgctgccct ttgccaacag tgcctgatag ttgtggaaga
     5401 aatagagcgc aaaatcaaac aggctaccgg ggattgcctc accacgccag gtgacatgtt
     5461 tttccggcaa gtgcagaccg cgtacccgac aaatcaaaac cgctggattg ggcttgagct
     5521 gataaatttt gcctgcttca ttggtatagc tgatggtgcc gttaaccgca tcgcgcaggt
     5581 taatttgccc gtcgatcact ttgttccagt ctggtgccag tgaatcttcg aaatcggcca
     5641 taaagacttt cacattggcg ttcagtgcgt tgatcaccat cttgcgctca accgggccag
     5701 ttatctcgac tcgacgatct tgtaagtccg cgggaatacc acgaattttc cagtccgtat
     5761 cgcgaatgga agctgtttcc gaaataaaat caggcaaagt tccgttatca atatcttgct
     5821 gctgctgaat gcgcgctgcc agaagtttat tgcgttgtgg cgtaaaatgc gtcaccagct
     5881 cagtcagaaa ttctaccgct tcggcagtaa gaatttgctt ctcctgctcg ccatacggcc
     5941 ttatgaaagc cagttcatcg gttgttgttg cctgttcagt catcgtgcag ctcctcgtca
     6001 tggatccgaa aacttcccca ctgtgaacga aggatcgttg tgcgcttttc gttcagcaca
     6061 actaagacta ctcatttaaa atgcaaaatc aaaaacaatt tccattttaa ttaaaaacac
     6121 ttgataactt attgataaca atatgaataa aatttattca cctcctgagg tgcgtttcgg
     6181 aaataaaaaa ggcacccgaa ggtgcctgag gtaaggtgct gaatcgctta acgatcgact
     6241 atcacagaag attaatccag cgttggattc atgtgccgta gatcgtatgg cgtgatctga
     6301 tagacgtaat agttgagcca gttggtaaac agcaaattac cgtgactacg ccagctcgct
     6361 cgcggtgtat tttgcggatc attgtgcggg aaatagttat acggtacttc cgggcctagt
     6421 ccggcttcca catcgcggaa atattcctgc gccagcgttt gcgcatcata ttcgggatgg
     6481 cccgtcacaa aggcaatgcg cttatctttg ctggcaaaca gatatgcatc cccttcttcc
     6541 gtctctgcca gaatttccag atcggtgtaa tcacgaatca acgctgccgg aaagtcagca
     6601 tagcgcgaat gtggtgccag gaatgaatca tcaaagccac gcgtcagaag cgcatgagga
     6661 tggagaatat gatgctcgta aacgccagag agtttgtcgg tgcgagtttg cttaggaatg
     6721 ccgtagagga tattgagcgc ggcctgtacc gcccagcaga caaacagcgt cgaggtgacg
     6781 tgatctttcg accactccag cacctgtttg atctgcggcc agtaagcgac atcattaaac
     6841 tccaccaggc ccagcggcgc accggttaca atcagaccgt caaagttctg ttcctgaata
     6901 tcttcaaagt tacagtagaa gttgttcaga tgctctgcgg gcgtgttgcg cgattcacgg
     6961 gaatcgatgc gcaacagctg aatatcgacc tgcaaaggtg agtttgaaag caggcgcaga
     7021 aactgatttt cagtttcaat cttcttcggc atcaggttaa ggatcagaac cttaagtgga
     7081 cgaatttcct gaccagacgc acgagaagtt gtcatcacaa agacgttttc ttcacgcaag
     7141 aaattgacgg cgggtagctc gtccggcaca cgaataggca taacctgatt acctcactac
     7201 atacgcttat acgtttagac atccagatag ctgaagataa ccagaaaatt tgccaatgtc
     7261 gagcctgcat gttgaaggtg agaaagtttc aaggaaaagg ctacgttaga atataagaat
     7321 gacgaaaagg agagaaaatg gttattagta ttcgccgctc acagcatgag gaaggggagg
     7381 aactcgttgc gatttggtgt cgatctgtcg atgccaccca cgatttttta tcagcagagt
     7441 atcgggccga gctggaagag ctggttcgtt cctttctgcc ggaagcaccg ttgtgggtcg
     7501 cggttaatga acgggatcag ccggttgggt ttatgttgtt aagtgggcag catatggatg
     7561 cgctgtttat cgatcctgat gtgcgcggct gcggcgtggg tcggatgctg gtggagcatg
     7621 cgctctcgat ggcaccggaa ctgacaacca acgttaatga gcaaaatgag caggcggttg
     7681 ggttctataa gaaggtgggt tttaaggtta cgggacgctc tgaggtggac gatttgggga
     7741 aaccgtatcc gttgctgaat ctggcgtatg tgggggaata agccgcccct gccgtgcctg
     7801 cccaggctgg agaagacgcg ccagcgtcgc atccgacctt tttctgcacc agcgcaaaaa
     7861 ggcccagtct tccgactggg ccttctgctt aatttgatgc ctggcagttc cctactctcg
     7921 catggggaga ccccacacta ccatcggcgc tacggcgttt cacttctgag ttcggcatgg
     7981 ggtcaggtgg gaccaccgcg ctaaggccgc caggcaaatt ctgttttatc agaccgcttc
     8041 tgcgttctga tttaatctgt atcaggctga aaatctttct ct
//
LOCUS       DAPHDW010000175         8026 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.178, whole genome shotgun sequence.
ACCESSION   DAPHDW010000175 DAPHDW010000000
VERSION     DAPHDW010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.178"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            489..1772
                     /gene="ydfJ"
                     /locus_tag="RH057_004009"
                     /pseudo
     CDS             489..1772
                     /gene="ydfJ"
                     /locus_tag="RH057_004009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
     gene            1861..3321
                     /locus_tag="RH057_004010"
     CDS             1861..3321
                     /locus_tag="RH057_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HDV3130300.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLDHPMVAADVQNPHQPKTETG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDEKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVGDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRYAAYGLMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(3357..3560)
                     /gene="ydfZ"
                     /locus_tag="RH057_004011"
     CDS             complement(3357..3560)
                     /gene="ydfZ"
                     /locus_tag="RH057_004011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HDV3130301.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(3738..4424)
                     /gene="ydfH"
                     /locus_tag="RH057_004012"
     CDS             complement(3738..4424)
                     /gene="ydfH"
                     /locus_tag="RH057_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HDV3130302.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(4513..5259)
                     /gene="ydfG"
                     /locus_tag="RH057_004013"
     CDS             complement(4513..5259)
                     /gene="ydfG"
                     /locus_tag="RH057_004013"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HDV3130303.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELKDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWCNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            5396..7441
                     /gene="dcp"
                     /locus_tag="RH057_004014"
     CDS             5396..7441
                     /gene="dcp"
                     /locus_tag="RH057_004014"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HDV3130304.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELLTRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAVAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDGNDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLYRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(7486..8004)
                     /locus_tag="RH057_004015"
     CDS             complement(7486..8004)
                     /locus_tag="RH057_004015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HDV3130305.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQTYTAVMHFAGDCETVLALAV
                     RFALVQLLQLLL"
BASE COUNT         2010 a         1838 c         2044 g         2134 t
ORIGIN      
        1 atggattttt atgctgatga agatgtttta tttggcgatt cgtcaagcat cttcacttaa
       61 gtaatcgtat attttaagag tgcaaaagag aaagggacaa cgttgctcgc actctggatt
      121 aaatataggg tgttgttaaa ctgcgttgat acataattgt accggatatt gagtggtctg
      181 aagtcccagt cattaagtaa tcgtttcgtg atatgccgca gaaacgttgt atgcaataac
      241 gttctgcggt tggctgatgc actttcgaga gtgtattgaa taatttcaag ccgttattgt
      301 ttttacgcgt aacaccatga aaagattgct tatctgttgg ggtgtttttt ggggcatata
      361 tgggacagaa ataggccaca gatagatagt aagatcgaac tcaggatgct tttgaaaaaa
      421 tgtaactatc tgaaaaaacc tagaaagcgc caaggaaacc acagaatgag aaaaaacatc
      481 tgtgaattat ggatttccag ttatattcgc tcggcgcagc gttggtgttt catgaaatat
      541 tttttcctga atcatcaacg gcaatggcgt taattttggc aatgggaacc tacggtgcag
      601 gttatgtggc gcgtattgtc ggagcattta ttttcggcaa aatgggcgac agaatagggc
      661 gtaaaaaagt gctctttatt accatcacca tgatggggat ctgtaccacc ttaattggtg
      721 tgttaccgac ctatgcacag attggtgttt ttgcccccat cttgctggtg acgctgcgta
      781 ttattcaggg gttgggtgca ggtgcggaaa tttccggtgc cggtacgatg ctggcggaat
      841 atgcaccaaa aggtaagcgc ggaattatct cctcatttgt agctatggga actaactgcg
      901 gaaccttgag cgcaacggca atctgggcct ttatgttctt cattctcagt aaagaggaac
      961 tgctggcgtg gggatggcgt ataccgttcc tggcgagcgt tgtcgtgatg gtctttgcta
     1021 tctggttacg tatgaatctg aaagaaagcc cggtctttga gaaggttaat gacagcaacc
     1081 aacccacagc aaaacctgca cctgctggta gcatgttcca gagcaaatcc ttctggctgg
     1141 caacagggct gcgttttggt caggcgggta actccgggtt aattcagact ttccttgcag
     1201 gctatttagt gcagacgtta ttgtttaaca aagcaattcc aacagatgca ttgatgatta
     1261 gttcgattct cggctttatg accattccgt tccttggttg gttatccgat aaaattggtc
     1321 gccggatccc gtatattatt atgaatacct ccgcgattgt gctggcatgg ccaatgcttt
     1381 ctatcattgt agataaaagc tatgccccga gcaccattat ggttgcactg attgtgattc
     1441 ataactgtgc ggtgctggga ttatttgctc tggaaaacat taccatggca gaaatgttcg
     1501 gctgtaaaaa ccgctttacc cggatggcca tttctaaaga aattggtggt cttatcgctt
     1561 ccggttttgg tcctatcctg gcgggtattt tctgcaccat gacggaatcc tggtatccga
     1621 tcgccattat gatcatggca tattcagtga ttggtttaat ctctgcgctg aaaatgccag
     1681 aagtgaaaga ccgtgattta agtgcgctgg aagacgctgc ggaagatcaa ccgcgtgttg
     1741 taagagctgc gcaaccttcc agaagtctgt aaatcctcaa tcccttctct taccggagag
     1801 gggattttta ttcatataac aaaacatgta gcttgccata tttaaattta aggaattgtt
     1861 atgggaaata atttgttatc agcaaaagcg acactccctg tttatgatcg taataacctg
     1921 gccccaagaa ttgttcattt aggctttggt gcatttcacc gtgcgcatca gggcgtgtat
     1981 gccgatattc ttgctacaga acatttcagt gactggggat attatgaggt caacttaatc
     2041 ggcggcgaac agcaaattgc cgatttacag cagcaagata atctttatac cgttgcggaa
     2101 atgtcggccg atgcgtggac ggctcgcgtc gttggcgtcg ttaaaaaagc cttgcacgta
     2161 cagatggatg gcttagaaac cgtgttggct gcgatgtgtg aaccgcaaat cgcgattgtc
     2221 tctctgacaa tcaccgaaaa agggtatttc cactctccgg cgaccggaca gttaatgctc
     2281 gatcacccga tggtcgctgc cgacgtgcaa aatccccacc agccgaaaac agaaacagga
     2341 gtgattgttg aggcgctggc tcgccgtaaa gcggcaggac ttcccgcatt taccgtcatg
     2401 tcatgtgaca acatgccaga aaacggtcat gttatgcgtg acgttgtcac ttcctacgcg
     2461 caagccgttg atgaaaaact ggcacaatgg atcgaagata acgtgacttt cccatcaaca
     2521 atggtggacc gtattgtgcc cgcagtgaca gaggatacgc tggcgaaaat cgaacaactt
     2581 accggtgtgg gcgatcctgc gggcgttgcc tgtgaacctt tccgccagtg ggtaatagaa
     2641 gataactttg ttgccggacg tccagaatgg gaaaaagcgg gagccgaact ggttagtgat
     2701 gtgctgcctt atgaagagat gaagttgcgc atgctcaacg gcagtcattc attcctggcg
     2761 tatttgggtt atcttgctgg atatcagcac attaatgact gcatggaaga tgaacattat
     2821 cgttatgcgg cgtatggctt gatgttgcag gaacaagcgc cgacgctgaa agtgcagggc
     2881 gttgatttgc aagattacgc taaccgatta attgcacgct atagcaaccc ggcgttacgt
     2941 catcgaacct ggcagattgc gatggatggt agccagaaat tgccacagcg gatgttggat
     3001 tctgttcgct ggcatctggc gcatgacagc aagttcgatc tgctggcgct gggcgtcgcg
     3061 ggttggatgc gttatgtcgg tggtgttgat gaacagggaa atccgataga aatcagtgac
     3121 ccactgttac ctgttattca gaaggctgta caaagtagtg ccgaagggaa agcgcgcgtc
     3181 cagtcattgc tggcgattaa ggcaatcttt ggtgatgatt tgccagacaa tagcttgttt
     3241 actgcaaaag tgacggaagc gtacttgtct ttattagcgc atggcgcgaa agcgaccgtg
     3301 gcgaaatatt ccgtgaagta aaaaaagcaa tgccgccatc ggcggcattc acacacttag
     3361 ttcatgccga gacgaatcag gtccagaggt gccagtttct cttcacaacc ttcaacaact
     3421 acggtttttc cgcggcggat ttgctcagcg gtcagacctt cagtatcaat cgacaggatc
     3481 ttgccagtgt gaccggtgcc gctaaccata acacggctgc cagtggtgat tgcgttacgg
     3541 ttacgatcgt aggtcgtcat aggtgttttc tcctttctga tttacagtcg aagcccacgt
     3601 aacgggcgca atataaatac tcctgtcgag tgaaatattt tttgtttttg atcaagctca
     3661 catctttttt gatgcatgca tgagccgtaa ggcgggggga aaagcctctc atcccggatg
     3721 ggatgagagt aaatgaatta ctcttcgcta aaccagtcgc tgttttcctg gcggatctgg
     3781 cgcacggatt cgctgatttc ctgcaaatgt tgcgtcattg cacgttctac cgcatcgcca
     3841 tcacgttttt gcagggcaga gaaaatatca agatgctggc gtaacagcat ttctggtgga
     3901 gaaacatggt cgaaactcat atagcgcacg cgatcaacgg tcgctttcag gttctcaatg
     3961 gtatcccacg ccagttgaca gtcggcaatc tgcgtcagga gttgatggaa gttgtcatca
     4021 agttcaaaaa aatcatccag ttgcttgcgc tcaatggcaa tgcgttgctg gtgaagattt
     4081 tgttccagtt ggtagcactg gctttcggta atcatgctcg ccgcccgacg cgccaccgcg
     4141 cactcaatgg cctgacggat aaaactgccg tttcgcacct gggccatgga aattttgttg
     4201 acgtagctgc cacgctgcgg acgaatttga atcaggccgt tttccgccag tttaataaag
     4261 gcttcacgaa ccggctggcg tgacacattg aaacgaacag aaacttcttt ttccgacaac
     4321 ggtgtgcctg gtgcaatcag gcaatggaca atgtcgcgac gaagaatacg ataaatctgc
     4381 tgattgacag gctgtgtggg attaagttgc gtttcgacgg tcattcgttc ttgcttgtga
     4441 gtgagttaac tgcatgagtc taccactttt ttgtgacaag caataacccg gcagttacgc
     4501 cgggtataaa aattactgac ggtggacatt cagtccggca tagctttggg taaccggcat
     4561 catttccagg gtattgatat tgacgtgagc aggcagcgtt gacacccacc agacggcttc
     4621 gctgacatct tctggcgtca atgcaacggt attttgatag gttttttccg ctttaccgtc
     4681 atcgccttta aagcggacat tggaaaactc ggtgccaccc accagacccg gttcgatgtc
     4741 ggtgacgcgc accgccgtac catgcaggtc cgtacgcaga ttcaggctaa actgacgaac
     4801 aaacgctttc gtcgcaccgt aaacgttacc accggcataa ggccagctac ctgccgttga
     4861 gccaatgtta ataatatgac catgattacg ttcaaccata cccggtaaga cggcgcgcgt
     4921 catatatacc aggcctttgt tgttggtatc aatcatcgtt tcccagtctt caacgctggc
     4981 tttatgcgca ggctccatgc ccaacgccag gccggcatta tttaccagga tatcaatatt
     5041 gcaccactcg gcagggagcg atgccagcat ctcttcaata gcggcgcggt tgcgaacgtc
     5101 cagttgggcg atatacagat tatctcccag ttcgtctttt aactcctgca accgctcttg
     5161 gcgacggcca gtggcgataa ctttatgccc ttgttgaata aaacgacgag taatgcattc
     5221 accaaaacct gccgttgctc cagttactaa aacgatcatc tcactgttcc tcaacgcttt
     5281 tgtgtgtcaa taacatagca cgtgatgctt aagagcgggt aatactggtt tgcaagattg
     5341 cactccgtta tcagtaagcc tacgctatgg aacacctcgc gttcaggaga agaaaatgac
     5401 aacaatgaat cctttccttg tgcaaagcac actgccgtat ctggctcctc attttgatca
     5461 aattgccaat catcactatc gcccggcatt cgatgaggga atgcagcaaa agcgggcaga
     5521 aattgctgcc atcgcgctta acccgcaaac gcctgatttc aacaatacta ttctggcact
     5581 ggaacaaagc ggagaattac ttacccgcgt taccagcgtc ttttttgcga tgactgcggc
     5641 gcataccaat gatgaattac agcgtcttga tgaacagttt tccgctgaac tggcggaact
     5701 ggctaatgat atctatctga acggtgaatt attcgcgcgg gtagatgctg tctggcagcg
     5761 ccgtgaatcc ctggggcttg atagtgaatc catccgcctg gtggaggtta ttcatcaacg
     5821 ttttgtcctt gccggagcca aacttgcgca agctgataaa gcaaaattaa aagtactgaa
     5881 tacagaagct gcgaccctga ccagccagtt taaccagcgg ttacttgcag caaataaatc
     5941 cggcggtctg gttgtgaacg atatcgcgca gctggcagga atgagtgagc aagagattgc
     6001 gctggcggca gtggcggctc gcgagaaagg tctggataac aaatggctga ttccgctgct
     6061 gaataccacc caacaaccgg cgcttgccga aatgcgcgat cgtgcgacgc gtgaaaaact
     6121 gtttattgcg ggctggacgc gagcggaaaa aaatgatggc aatgataccc gcgctatcat
     6181 tcaacgtctg gtagagattc gcgcacagca ggcgaaactg cttggttttc ctcattatgc
     6241 cgcatggaaa atcgccgatc agatggcaaa aacacctgaa gcagcactca actttatgcg
     6301 ggaaattgtt ccagcggcgc gtcaacgtgc tagcgatgaa ttagcctcca tacaggcggt
     6361 tatcgataag cagcaaggcg ggtttagcgc gcagccgtgg gactgggcat tttatgccga
     6421 acaggtacgg cgggagaaat ttgatcttga tgaggcgcag ctcaagccat attttgaatt
     6481 aaacacggtg ttgaatgaag gtgtattctg gaccgcgaat cagctcttcg gtattaagtt
     6541 tgtcgaacgt tttgatattc ctgtctacca tcctgacgtt cgtgtgtggg aaatttttga
     6601 tcataatggc gtgggactgg cgttatttta cggtgatttc ttcgcccgtg attcaaaaag
     6661 cggcggtgca tggatgggca attttgttga gcaatcaacg cttaatgaaa cgcatccggt
     6721 aatttataac gtttgcaatt atcagaaacc cgctgccggt gagcctgcgt tgttactctg
     6781 ggatgatgta ataaccttat tccatgaatt tggtcatacg ctgcacggcc tttttgcccg
     6841 ccagcgttat gccacgcttt ccggcaccaa cacgccgcgt gattttgtcg aatttccgtc
     6901 gcaaatcaac gaacactggg caacgcatcc gcaggtattc gctcgctacg cccggcatta
     6961 tcagagcggg gcagcaatgc ctgacgaact gcaacagaaa atgcgtaatg ccagcctgtt
     7021 caacaaaggg tatgagatga gcgaactgct tagcgccgca cttctcgata tgcgctggca
     7081 ttgcctggaa gaaaacgaag caatgcagga tgtcgatgat tttgaattgc gggcgctggt
     7141 ggcggaaaat atggatcttc ctgctatacc gccacgctat cgcagcagtt atttcgccca
     7201 tatttttggt ggcggatatg ctgcaggtta ttacgcttat ctgtggacgc aaatgttggc
     7261 cgatgatggt taccagtggt ttgttgagca gggcggatta acgcgtgaaa atgggcagcg
     7321 ttttcgcgag gcgatccttt ccagaggtaa cagcgaagat ctggaacgcc tgtatcgaca
     7381 atggcgcggt aaggctcctc aaattatgcc gatgctgcaa catcgtggct tgaacatata
     7441 aacaccacca atggctggcc tggagatatc gccaggccag cctggttata gcagtaattg
     7501 cagcagttgg acgagggcaa acctcaccgc taaagctaat accgtttcgc agtcgccagc
     7561 aaaatgcata accgcagtgt aggtctggcc tttaatatgc cacgcgaacc agacggtacc
     7621 cgctggcgta ccatcttcac cgccctccgg tccgccgtag ccggtaatgg caatactgac
     7681 atcagcatcc gcacgctcta tggcacctgt tgccatttct gctgccactt tctcactcac
     7741 cgcagaatat cgttcaagag attgctggct tacgctgagg attttcatct ttgcctgatc
     7801 ggtgaaagta acaaagcctg caccgtaaaa tttgggtgta tcttcagctg cacacagggc
     7861 gctagccaac ttaccgccgg tgcatgattc ggcggtagtt aagcgtaatt gatccgcgat
     7921 aagtctttga ctcaacgcgg atgtcaggtt ttcaatagtg tcggtatctg cgagttgtac
     7981 aattttatct ttattaatgt tcactgtttt ttgtccacgc tattat
//
LOCUS       DAPHDW010000176         8020 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.179, whole genome shotgun sequence.
ACCESSION   DAPHDW010000176 DAPHDW010000000
VERSION     DAPHDW010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.179"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..840
                     /gene="potE"
                     /locus_tag="RH057_004016"
     CDS             <1..840
                     /gene="potE"
                     /locus_tag="RH057_004016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HDV3130306.1"
                     /translation="VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFL
                     GLESACANTDVVENPERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFG
                     LAFAQMFTPEVGKVIMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVD
                     APVQGMLTIVIIQSGLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVA
                     NVPPSKAKVANFVAFVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKN
                     KHG"
     gene            complement(896..2536)
                     /gene="pgm"
                     /locus_tag="RH057_004017"
     CDS             complement(896..2536)
                     /gene="pgm"
                     /locus_tag="RH057_004017"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HDV3130307.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAISNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDRANALMADGLKGVKRISLDEAMASGHVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(2562..3107)
                     /gene="seqA"
                     /locus_tag="RH057_004018"
     CDS             complement(2562..3107)
                     /gene="seqA"
                     /locus_tag="RH057_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HDV3130308.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            3292..4056
                     /gene="ybfF"
                     /locus_tag="RH057_004019"
     CDS             3292..4056
                     /gene="ybfF"
                     /locus_tag="RH057_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HDV3130309.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPRDPVMNYPAMAQDLVDTLDAQQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            4196..4489
                     /gene="ybfE"
                     /locus_tag="RH057_004020"
     CDS             4196..4489
                     /gene="ybfE"
                     /locus_tag="RH057_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HDV3130310.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            4629..5159
                     /gene="fldA"
                     /locus_tag="RH057_004021"
     CDS             4629..5159
                     /gene="fldA"
                     /locus_tag="RH057_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HDV3130311.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            5369..5455
                     /gene="uof"
                     /locus_tag="RH057_004022"
     CDS             5369..5455
                     /gene="uof"
                     /locus_tag="RH057_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HDV3130312.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            5448..5894
                     /gene="fur"
                     /locus_tag="RH057_004023"
     CDS             5448..5894
                     /gene="fur"
                     /locus_tag="RH057_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HDV3130313.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(5979..6305)
                     /locus_tag="RH057_004024"
     CDS             complement(5979..6305)
                     /locus_tag="RH057_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130314.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(6355..7761)
                     /gene="chiP"
                     /locus_tag="RH057_004025"
     CDS             complement(6355..7761)
                     /gene="chiP"
                     /locus_tag="RH057_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HDV3130315.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSLGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWVKADGQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFGAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
BASE COUNT         1921 a         2020 c         2017 g         2062 t
ORIGIN      
        1 gtggtaggcc tgtgcattat cggctggttc tggtttagcc cgacgcttta cgttgattcc
       61 tggaatccgc atcatgcacc gttcttcagt gcagtaggtt cttccatcgc tatgacgctg
      121 tgggcttttc tcggtctgga gtctgcgtgt gcgaatactg atgtagtgga aaacccggaa
      181 cgtaatgtgc caatcgcggt actcggcggt acgttaggtg cggcggtgat ttatatcgtc
      241 tccaccaacg tgattgcagg gattgtgcct aatatggagc tggcaaattc aacggcacca
      301 tttggcctgg ccttcgcaca gatgttcaca ccagaagtgg gtaaagtcat tatggcgctg
      361 atggtgatgt cctgctgcgg ttcgctgctt ggctggcagt tcaccattgc ccaggtgttt
      421 aaatcttcat ctgatgaagg ctacttccct aaaattttct cccgtgtaac caaagtggat
      481 gcaccggtgc agggaatgtt gaccattgtg attatccaga gcgggttggc gctgatgacg
      541 attagcccgt cgctaaacag tcagttcaac gtgctggtta acctggccgt ggtgaccaat
      601 atcattccgt atattctgtc aatggcggca ttagtcatta ttcagaaggt cgctaatgtg
      661 cccccctcaa aagcgaaagt tgcaaacttt gttgcttttg ttggcgcgat gtatagcttt
      721 tatgcgctgt actcatccgg ggaagaagcc atgctgtacg gttccatcgt gaccttcctt
      781 ggttggacac tgtatggtct ggtatcacca cgctttgaac tgaaaaataa acacggttaa
      841 aaaagcaaag taaaaaaggg cgatcttgcg accgcccttt ttttattaaa tgtgtttacg
      901 cgtttttcag aacttcgcta acaatctcaa ccgcttcttt ctcaatctgc ttgcgatgtt
      961 cttcaccgag gaagctttcg cagtagatct tatatgcgtc ttccgtgcct gacggacgcg
     1021 cggcgaacca gccgttgtcg gtcatcactt tcagaccgcc gatagaagca ccattgcccg
     1081 gtgccgccgt caggcgcgcg gtgatcgggt cgcctgcaag ggtgctggcg ctcaccattt
     1141 ccggagacag cttagacagc gccgcttttt gtgcggaagt cgcggctgcc tgcaaacggt
     1201 tgtagctcgg cgcaccaaag cgttttgcca gttcgttgta gtgttcctgc gggttcttac
     1261 cggtgacagc ggtgatttcc gccgccagca gacacataat gatgccgtct ttgtcggtgg
     1321 accacggcgt gccgtcgaaa cgcaggaagg aagcccctgc gctctcttcg ccgccaaagc
     1381 cgaagctgcc gtcgaacaga ccatcaacaa accatttgaa acctaccggg acttctacca
     1441 gcttacggcc caagtcattg accacacggt cgatcatcgc agaagaaacc agcgttttac
     1501 cgacagcaac atctttgccc cactgcggac gatgctggaa caggtagttg atcgccaccg
     1561 ccaggtagtg gttcggattc atcaaacctg ccggagtgac gataccgtga cggtcataat
     1621 ccgggtcgtt agcaaacgcc agatcgaact tatcacgcag tgccagcagg cccgccatcg
     1681 cacactcgga ggagcagtcc atacggatcg cgccgtcttt atcaaggtgc ataaagcgga
     1741 aggtttgatc gacctgatcg ttaacgatgg tcaggttgag gttgtaatac tcgccaatac
     1801 gcttccagta ttcgataccg gaaccgccca gcggatcaac gcccagcgtc aggcccgctt
     1861 tctgaattgc cgccatatca acgatatcgg ccagaccttc cacaaacggc tgcaccagat
     1921 cctgctcttt tacatgaccg gatgccatcg cttcgtcgag ggagatacgc ttcacgcctt
     1981 tcaggccatc ggccatcagt gcgttggccc tgtcttccac cactttggtg acgttggtat
     2041 cagccgggcc accatttggc gggttgtact tgataccacc atcttccggc gggttatggg
     2101 acggtgtaat cacgataccg tctgccagcg ggccaccttt tttattgtga accaggatgg
     2161 cattggaaat ggcaggtgtc ggagtgaagc cattgttttc ctgcacaatg acatcaacgc
     2221 cgttcgctgc cagcacttcc agtacggaaa tgaacgcagg ctcggacagg gcgtgagtat
     2281 ctttacccac atagcaaggg ccagtgatgc cgtttttcgc acgttcttca gcaattgcct
     2341 gagcgatcgc cagaatgtgc ggctcgttaa agctgtggcg cgctgcactg ccacggtgac
     2401 cggaagtacc gaatttcacc gcgtgctccg cattccctgc ttctggtttc agtacataat
     2461 attgcgccgt cagttgggcg acgttaatca aatcactctg ttgtgcaggt tggcctgcac
     2521 gattgtggat tgccattgct ttgtcctttg tctgcaacgt tttagatagt tccgcaaacc
     2581 ttctcaatca attccgccgg gaattgcatc gactgcatga tgtgttcgat catgctgcat
     2641 ttacggccgg tgttggtgtt ggtgatcacc caatacggcg tgcctggcac atgtttcggc
     2701 ttggtctgat taccattttt cagcagcgtt tgttcatctg ccgcaaagta aacgcgtgta
     2761 cgaccgtgca acgattccgt tgcttcggca aacgcctggg cgtcaagaga atatagtgta
     2821 gacaacagca gcataaagcg attgaccgct cgcttttgct ctgcgtattc atccgaaagc
     2881 agaagttcac gcattgcgcg aaccttgtct ttaatcgttt tgaccggctt cgcttcgacg
     2941 atagcaggtg acgcaacgcg aacctctttc gtcaccggag cagcaggctg tgatgcggcg
     3001 gaaaatttca acatacgccg taaaatgtcg gatgcgctct cgccgatatg cttagtgtgg
     3061 ctggcaatat agctgtagag ttcatcatca acttcaatcg ttttcatctt aatccagtgc
     3121 agtgtcttag ctgaatacaa gtcgccagga gtatatgaat aaatcccggc agcggatagc
     3181 gtcaagcctg gcttgcagaa aaactcggaa taaaccttat ttgttgcagc cctgtggcac
     3241 aatggtcaac ataataccct aacccgacaa cgcgaaaaaa agaactttgc catgaaattg
     3301 aatatccgcg cgcaaactgc acaaaaccag cacaataatt ctcccatcgt tcttgtccat
     3361 ggtctgtttg gtagcctcga caaccttggc gtactggctc gcgatctggt aaacgatcac
     3421 aatatcatcc aggttgatat gcgtaaccac ggtctttcac cgagagatcc ggtaatgaat
     3481 tacccggcga tggctcagga tcttgttgat accctggatg cacagcagat cgacaaagca
     3541 acatttatcg gtcactccat gggcggtaaa gcggtaatgg cacttactgc actggccccc
     3601 gatcggattg acaagctggt ggcaattgat attgctccgg tcgattatca cgttcgccgg
     3661 catgatgaga ttttcgcggc catcaacgca gtcagtgaat cggacgcaca aactcgccag
     3721 caagcagcgg caataatgcg ccagcatctt aatgaagaag gggtgattca gtttctgctg
     3781 aaatcttttg ttgacgggga gtggcgcttt aacgtgccgg tattgtggga tcagtatccg
     3841 catattgtgg ggtgggagaa aatcccggca tgggatcacc ctgccctgtt tatccctggc
     3901 ggcaattctc cgtatgttag cgagcagtac cgtgatgatt tactggcaca atttccacag
     3961 gcacgggcgc atgtgattgc aggcgcgggt cactgggtcc atgctgaaaa accggatgcg
     4021 gtattacgcg ctatccgtcg ctatctcaat gattaacgca actgattaaa aacccagcgt
     4081 ccgcgtcgct ttccgcgcgt gggcgttggc gtgactcatc gcgctgatgt attatggcgc
     4141 gctatccatc cgggcagagg cctggctgat tgtttccccc gaagtcacca agatcatggc
     4201 caaagaacaa acggaccgta cgacattaga tctgttcgcg cacgagcgtc gaccgggacg
     4261 accgaaaact aatccgcttt cgcgcgatga acagctgcgt attaataaac gcaaccagct
     4321 aaaacgcgac aaagtacgtg gccttaagcg tgtcgaactg aagctgaacg cggaagctgt
     4381 cgaggcgctg aacgagctgg cggaatcgcg caatatgagc cgtagcgaac tgatcgaaga
     4441 gatgttaatg cagcaactgg cggcgctgcg tagtcagggc atcgtttaaa tttccagttt
     4501 catgtagcac agtgtgcagt cctgctcgtt tgctgattcc gcaagactgc ctgttctgct
     4561 atgattgcct ttatccgtgg gcaattttcc acccccattt caataagttt caagaggtta
     4621 tttcactcat ggctatcact ggcatctttt tcggcagcga caccggtaat accgaaaata
     4681 tcgcaaaaat gattcaaaaa cagcttggta aagacgttgc cgatgtccat gacattgcaa
     4741 aaagcagcaa agaagatctg gaagcttatg acattctgct gctgggcatc ccaacctggt
     4801 attatggcga agcgcagtgt gactgggatg acttcttccc gactctcgaa gagattgatt
     4861 tcaatggcaa actggttgcg ctgtttggtt gtggtgacca ggaagattac gccgaatatt
     4921 tctgcgatgc attgggcacc atccgcgaca tcattgaacc gcgcggtgca accatcgttg
     4981 gtcactggcc aactgcgggc tatcatttcg aagcatcaaa aggtctggca gatgacgacc
     5041 actttgtcgg tctggctatc gacgaagacc gtcagccgga actgaccgct gaacgtgtag
     5101 aaaaatgggt taaacagatt tctgaagagt tgcatctcga cgaaattctc aatgcctgat
     5161 gtgatgcggc gtagactcat gtctacgccg tattaataga taatgccaat caaaataatt
     5221 gctacaaatt tgtaactttt gctgttgtac ctgtacaatg tcccggtgtt caagtggcct
     5281 tgccgttgta aatgtaagct gtgccacgtt tttattaaca atatttgcca gggacttgtg
     5341 gttttcattt aggcgtggca attctataat gatacgcatt atctcaagag caaattctgt
     5401 cacttcttct aatgaagtga accgcttagt aacaggacag attccgcatg actgataaca
     5461 ataccgccct aaagaaagct ggcctgaaag taacgcttcc tcgtttaaaa atcctggaag
     5521 ttcttcagga gccggacaac catcacgtca gtgcggaaga tttatacaaa cgtctgatcg
     5581 atatgggtga agaaattggt ctggctacgg tatatcgcgt actgaaccag tttgacgacg
     5641 ctggtatcgt cacccgccac aattttgaag gcggtaaatc cgtatttgaa ctgacacagc
     5701 aacatcacca cgatcacctg atctgcctcg actgcggcaa ggttatcgaa tttagtgatg
     5761 attccatcga agcgcgtcaa cgtgaaattg ccgcaaaaca tggcattcgc ctgactaacc
     5821 acagtctcta tctttacggt cactgtgccg aaggcgattg ccgcgaagat gaacatgcgc
     5881 acgaaggcaa ataagccagc ctgaatgaga aaaagccaac ctgcgggttg gcttttttat
     5941 gcaagggaaa taagaaaagc gagagttaca gctctcactt atttgttatt actgcgaatt
     6001 tcctgccaaa ctttatcaca atcggctttc accgcctgat catttccggt ttgcgttgca
     6061 cgcagacact gctgataatc cagtacgcgt acactttcct ggtcggcaaa ctgctgatgt
     6121 tgtttctctt tcttcagcac gtttaacacg ctctggcagg cttcaatttt ttctggcgaa
     6181 ccttgcgcgg tgttgataca ggcgctgtat gcctctttca gacggctgtc ttcctgtggt
     6241 gcggttgatt gcgcacaagc taccagcccc gatgccatca tggcgatgag aatcagtttt
     6301 ttcattgaaa attcctcaaa cgtgcggcag gcacaaaacc tgccgcgtcg ggcatcagaa
     6361 gatggtgaat ggtgcgatta ccataaattt cacgtcacgc tcatcctgga agatgttacc
     6421 gtaaccgccg ccccagcttg ggatgtcgga gtggttgtcg tattcggtga agtgcagttt
     6481 gaacatcgtg cctttggcgc gaccgtcctg aatggtgtag accgcatcca ggctgtatgc
     6541 agactcttca atagtccggt ttttgtcgta gtacgcatcc ggattgctct gccaggtcgc
     6601 aggtttagca tcccatgcgt aaacgtagga agcgccgatg gcgaagcctg gaagattcca
     6661 gtttttcagg tcatacatcg caccgaagaa gaccgctttt tcgccgttgg cgttgaagtc
     6721 ggaacggtta tcccaccaga tatccaggcg gccgttggag gaagcgtagg ttggagtcat
     6781 acgttgcagg aagtatccct gctgaccgtc agccttaacc caggtgcctt cgaggcgcaa
     6841 atccactacg tcagccgccc ggtaaccaaa ggtcaacgct tgcagccagg cggtgccgtc
     6901 ataaaggtcg ttgacgctgc gatcgtcaac tttatcgcgc gtaccataga actggtagct
     6961 ggtggttaac gggctaccgg cgatatcaaa tttgtagctg gctttggcaa aatattgatc
     7021 gatataccct tccgcctgac caaatgccgc ttccagtacg aagttatttt tgaagtcgta
     7081 tttcgcccca agggagtgca gataatcaac tttggtggtt ttatcgttct gataaaactc
     7141 atccatttcc agatgccacg gcgctttgta ttcgttggtc cacatgtagg agaaactcaa
     7201 ggcaccagca tcgccgtaat caaaattcgc cccggcttcc gccccctgat aagtacctgg
     7261 cataaagctc cagtgcggcg ctaacagcgt ttgaccagtt ggctgaatgt aacctgccct
     7321 cgcccaaacc ggaccgtatt taaatttggc cgcagcttta tacaggctta taccgctttt
     7381 gtcgccggac cagtcttcgt cataggcttt attacttttt gaaaacgcga tttcgttcgg
     7441 gtgggagctg tcgccgtttt ccgccatttc aatcgccgta aacgcagcaa tatcaaggcc
     7501 gaacatatca gcagcataac cggactgaaa atcgaggttg gcattccagg tggagtgaga
     7561 aaggttggtt ttgtatttgt cgccgtcggt aacatcttta cggtcgcgtt cacgctgcca
     7621 gtaatatata ccgccggtta aggttgaatc gtcgatgaat ccttcggcgc gagcctccgg
     7681 cattgccata aagcccgaca ttgctgtaac accggcgata gccagcgcca gcgtactacg
     7741 tttgccacta aacgtacgca tgggttaatc ctctttgacg tataaattgc tgcaccaaag
     7801 gtgaacagcg aaaaaataaa aattaaaaaa gtagcgttga gaaaagtctc gctgactaca
     7861 ggaacagact atttttcgcg acgcgaatta tcaatctctt tctgtaacca gaatgtaaga
     7921 acatgagcaa ccgcacatat tttgatgatt tttgttacaa ggcttaagtt tgcggttgaa
     7981 aggcttgcag aagaataaaa gcgtgaattt ccagcaagat
//
LOCUS       DAPHDW010000177         7888 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.180, whole genome shotgun sequence.
ACCESSION   DAPHDW010000177 DAPHDW010000000
VERSION     DAPHDW010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.180"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            210..1115
                     /locus_tag="RH057_004026"
     CDS             210..1115
                     /locus_tag="RH057_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130316.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLTQKGFCLALE
                     LGPHTYVGEHQLICRKGESANVKRVGLDNRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRADDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            1132..1494
                     /locus_tag="RH057_004027"
     CDS             1132..1494
                     /locus_tag="RH057_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HDV3130317.1"
                     /translation="METRLNLLCEAGVIDKDICKGMMQVVNVLETECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQVLLKEFALEVNPCEE
                     GYLLANLYGLWMAANEEV"
     gene            1578..2741
                     /locus_tag="RH057_004028"
     CDS             1578..2741
                     /locus_tag="RH057_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HDV3130318.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQVLENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLSVAHQ
                     GHLVQIPCHQVADAVEQGTDVITVFTLDKAREVSAAAVKAGRVQFVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVVAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLIQAR
                     DQLAKSGIALEQLNAPSATSCTSLPLLAEYGVTHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIHRGEPEIVGRYDSLGNSLGA"
     gene            2741..3967
                     /locus_tag="RH057_004029"
     CDS             2741..3967
                     /locus_tag="RH057_004029"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HDV3130319.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPALEKLGLINALGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIGRVEQALVSAGWQVERRGDDLQFLWVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVREQVQVGRVITFGGLLTDSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDKKVQVPQKLYEAGVPTVLVGKVADIVNNPYGVSWQNLVDSQRIMD
                     ITLNEFNTHPTAFICTNIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGMIATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            3964..4842
                     /locus_tag="RH057_004030"
     CDS             3964..4842
                     /locus_tag="RH057_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HDV3130320.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGTSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRETPSQFFQ"
     gene            4853..5206
                     /locus_tag="RH057_004031"
     CDS             4853..5206
                     /locus_tag="RH057_004031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HDV3130321.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGSFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            5218..6522
                     /locus_tag="RH057_004032"
     CDS             5218..6522
                     /locus_tag="RH057_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HDV3130322.1"
                     /translation="MDLYIQIIVVACLTGMTSLLAHRSAAVFHDGIRPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLI
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            6534..7619
                     /locus_tag="RH057_004033"
     CDS             6534..7619
                     /locus_tag="RH057_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HDV3130323.1"
                     /translation="MKTFPLQSLTIIEAQQKQFALVDSICRHFPGSEFLTGGDLGLTP
                     GLNQPRVTQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITVDFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVINRIRA
                     TLYSGGSQIQGAQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVPEVKSAVIANA
                     QSKVLIVEFHQPIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQSE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(7726..>7888)
                     /locus_tag="RH057_004034"
     CDS             complement(7726..>7888)
                     /locus_tag="RH057_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HDV3130324.1"
                     /translation="KFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISISKI
                     IGGCGPTAQ"
BASE COUNT         1806 a         1871 c         2280 g         1931 t
ORIGIN      
        1 caggcaagcg ccagtgggaa ttaccctttg ctcgcattgt cgaataaatc acttcgtcat
       61 cactctgaaa ccggggaaac ccggtttttt tatcctcatt tgcaataatc cgaaaaaatg
      121 tgaagcgcct cgccgtttcc acatctcaca gttgccactt atcttcattt taatgaagat
      181 ataaacattc attttattga aaatatatta tgcgtcgaac gtttatcaaa aaagaaggcg
      241 tcgttatcac gacgctggcc cgttatttgt tgggtgaaaa atgcggtaat aggttgaaaa
      301 ccatagatga gctggcaact gaatgccgtt catccgttgg cctgacgcag gccgcgttga
      361 aaacgctgga atcaagcgga gcgatacgga ttgaacgccg tgggcgcaat ggcagttatc
      421 tggtcgagat ggataacaaa gcattgctga gtcatgtgga tatcaacaac gtggtatgtg
      481 cgatgccttt gccctatacc cgcttgtacg aaggtttagc gagcggattg aaagcccagt
      541 ttgatggcat tcctttttac tatgcgcata tgcgcggcgc ggatattcgc gtggagtgtt
      601 tacttaacgg cgtgtatgac atggcggtgg tttcccgact ggcggcggaa agttatctca
      661 cgcaaaaagg gttttgcctc gcgctggagt tggggccgca cacctacgtt ggcgagcacc
      721 agttgatttg ccgtaaaggc gagtccgcaa acgtgaagcg cgtggggctg gataaccgtt
      781 cggcggatca gaaaatcatg accgatgtct tttttggcga tagtgatgtg gaacgtgtcg
      841 atctctctta tcacgagagt ttacaacgca ttgttaaagg cgatgtcgat gcggttatct
      901 ggaacgtggt ggcggaaaac gaactgacca tgctgggatt agaggcgacg ccgctcacag
      961 acgatccgcg atttttacag gccaccgaag ccgtggtcct gacgcgagcc gatgattacc
     1021 caatgcaaca actgctgcgt gccgttgtag ataaacacgc cctgctcgcc catcaacaac
     1081 gcgtggtgag tggggaacag gaaccgagtt attaaacgaa aggcatctga tatggaaacc
     1141 agactcaacc tgctttgcga ggcaggcgtt attgataagg acatctgcaa aggcatgatg
     1201 caggtcgtca acgtactgga aacagagtgc catctgccgg tgcgcagtga gcaaggaacg
     1261 atggcgatga cacatatggc gagtgcactg atgcgcagtc gccgtggtga agaaatagag
     1321 ccgctggata acgaattgct ggcagagctg gcgcaatcca gccactggca agccgttgtg
     1381 caattgcatc aggtgttgtt gaaggaattc gcactggaag ttaacccgtg tgaagaaggc
     1441 tatttgctgg ccaaccttta tggattatgg atggctgcta acgaagaggt ttgaagctcc
     1501 cgacaaatcc acgtcgcaca acctaaatat tcaaaaaaat gaatatttaa ttcaaggaac
     1561 agaaacgagg caacaagatg tttgtagaag cattgaaacg ccagaacccg gcgctgattt
     1621 ccgccgcact aagtttgtgg cagcagggca agatcgcccc ggacagctgg gtgatcgacg
     1681 tggatcaggt actggaaaac ggtaagcgac tgattgagac ggcgcggctt tacggcattg
     1741 aactgtatct gatgaccaag cagtttggcc gtaacccatg gctggcggaa aaattgctgg
     1801 cattaggtta cagcggcatt gtggcggtgg attacaaaga ggcgcgagtc atgcgccgcg
     1861 ctggtttgtc ggtggcgcat caggggcatc tggtacaaat cccttgtcat caggttgctg
     1921 acgccgttga acagggcacc gacgtcatca ccgtgtttac tctcgacaaa gcgcgggaag
     1981 tttctgcggc agcggtgaag gccggacgtg tgcagtttgt gctgctcaaa gtttatagcg
     2041 acgatgattt tctttatccg gggcaggaga gcggttttgt acagcattcg ctgcacgagg
     2101 tggttgctga aattaagaaa ctgccggggc tgcatttagc cggacttacc catttccctt
     2161 gcctgctttg ggatgaggct gccggaaaag ttttgccgac accgaatctt cacacgctga
     2221 tacaggcacg ggatcaactg gcgaaatctg gtattgcact tgagcaactg aacgcgcctt
     2281 cagcgaccag ctgcacttcg ctgccattgc tggcggaata cggtgtgact catgccgaac
     2341 ccggtcatgc gctgacgggc actattccgg caaaccagca gggcgatcag cctgaacgta
     2401 tcgcgatgct ctggttaagt gaaatctccc atcatttccg tggcgacagc tactgctacg
     2461 gcggcggtta ctaccgtcgt ggtcatgcgc aacatgcgct ggtgtttacg ccagaaaatc
     2521 aaaagattac tgaaaccaat ctcaaaactg tggatgacag cagtatcgac tacaccctgc
     2581 cgctggcagg cgagtatccg gtgagcagtg cagtggtgct ctgtttccgc acgcagattt
     2641 ttgtcactcg tagcgatgtg gtgctggtgt ccggtattca ccgtggcgaa ccagaaatcg
     2701 tcggtcgtta tgacagtctt ggaaactctc tgggggcgta atggcgcgat ttgtggtgtt
     2761 agtgattgat agctttggcg taggggcaat gaaagatgtc acgctggtgc gcccgcaaga
     2821 tgccggggcg aatacctgtg gtcacatcct gagccagttg ccgcatttgc agctaccagc
     2881 gctggagaag ctggggctaa tcaacgcatt gggttatgca ccaggtgata tgcaaccgtc
     2941 agattccgca acctgggggg tggcagagct gcaacatgaa ggtggcgata cctttatggg
     3001 gcatcaggaa attttaggca cgcgcccgtt accgccgctg cggatgccct ttcgcgatgt
     3061 gattggccgt gttgagcagg cattagtttc cgctggctgg caggtggagc gccgtggcga
     3121 tgatctgcaa tttctgtggg tcaatcaggc ggttgcgatt ggcgataatc tcgaggcgga
     3181 tttaggccag gtctataaca ttaccgccaa tctctctgtg atctcttttg acgacgcaat
     3241 caaaattggt cgtatcgtgc gtgagcaggt acaggtcggt cgggtcatta catttggtgg
     3301 cctgttaacc gacagtcaac gcattctcga tgccgcagaa agcaaagaag ggcgctttat
     3361 tggtatcaat gcgccgcgtt ctggcgctta tgacaacggt ttccaggtcg tgcatatggg
     3421 ctatggcgtc gataaaaaag tgcaggtgcc acaaaaactg tatgaagcag gcgtgccaac
     3481 cgtgctggtg ggtaaggtgg cagatatcgt caacaatcct tatggcgtga gctggcaaaa
     3541 tctggtggat agccagcgga ttatggatat caccctcaac gaatttaaca cccatccgac
     3601 ggcgtttatt tgcaccaaca ttcaggaaac cgacctcgct ggtcatgcag aagacgtcgc
     3661 acgttatgcc gaacgtttgc aggtcgttga ccgtaacctt gcccggcttg ttgaggcgat
     3721 gcagccagat gattgcctgg tcgtgatggc ggatcacggc aacgatccga ccattggtca
     3781 cagccaccat acccgcgaag tggtgccagt gctggtttat cagcaaggga tgatcgctac
     3841 gcagctcggt gtgcgcacca cgctttctga tgtgggggct accgtgtgtg aatttttccg
     3901 cgcgccaccg ccacaaaatg gtcgctcttt tctttcctcc ctccggtttg caggagacac
     3961 cctatgagtt ttgatccgac gggttacacc ctcgcccatg agcatctgca tattgatctc
     4021 tccggcttta aaaacaacgt ggactgccgc cttgatcagt atgcgttcat ttgccaggag
     4081 atgaacgacc tgatgacccg gggcgtgcgt aatgtgattg agatgaccaa ccgttacatg
     4141 gggcgcaatg cgcaatttat gcttgatgta atgcgcgaga cggggatcaa cgtggtggcc
     4201 tgtaccggtt attaccagga cgcgtttttc ccggaacatg tggtgacccg cagcgtgcag
     4261 gaactggcgc aggagatggt cgatgaaatt gaacagggta tcgatggcac ggagctgaaa
     4321 gccgggatca tcgcggagat cggcaccagc gaaggaaaga ttacgccgct ggaagagaag
     4381 gtatttattg ccgctgcgct ggcgcataac cagaccggac gcccgatctc cacgcatacg
     4441 tcgttcagca cgatggggct ggagcaactg gcgttgctac aagcccacgg ggttgatctt
     4501 tcgcgcgtca ccgttggtca ctgcgatctg aaagacaacc tcgacaacat tttgaagatg
     4561 atcgatctcg gcgcgtacgt gcagttcgac accattggta agaacagtta ctacccggac
     4621 gaaaagcgta ttgcgatgct tcatgcgcta cgtgaccgtg ggttgctgaa ccgcgtcatg
     4681 ctgtcgatgg atattacgcg ccgctcccat ttaaaagcca atggtggtta tggctatgac
     4741 tatttattga ccacctttat tccgcaattg cgccagtcag gattcagtca ggccgatgtg
     4801 gatgtgatgt tacgtgaaac tccctctcaa tttttccaat aaggacagac tcatgaaaaa
     4861 gattggcgtt gcaggcttac agcgtgagca gattaaaaaa accattgaag cgacggctcc
     4921 tggcagtttt gaagttttca ttcacaacga catggaagcg gcaatgaaag tgaaatccgg
     4981 gcaactggat tactacatcg gtgcgtgtaa taccggtgcg ggcgcggcat tgtcgattgc
     5041 catcgcggtg attggctata acaaaagttg caccattgcc aaaccaggca ttaaagcgaa
     5101 agacgagcat atcgccaaaa tgatcgctga aggaaaagtg gcgtttggcc tttccgttga
     5161 gcacgtcgaa cacgcgattc cgatgctgat taaccatctg aaataaaagg cacgactatg
     5221 gatctgtata ttcagattat cgtggtggcg tgcctgacgg gtatgacatc gcttctggcg
     5281 catcgctcgg cggctgtttt tcatgacggc atccgcccga tcctgccgca actgattgaa
     5341 ggctatatga accgtcgcga ggcggggagt atcgcttttg gtctgagcat tggttttgtg
     5401 gcctcggtgg ggatctcttt taccctgaaa accgggctgc tcaacgcatg gttactcttt
     5461 cttcctaccg atatcctcgg cgtcctggcg ataaacagcc tgatggcgtt tggtcttggc
     5521 gctatctggg gcgtgttgat ccttacttgc ctgttgccag taaaccagct gctgaccgcg
     5581 ctgccggtgg atgtattagg tagcctgggg gaattaagct cgccggtggt ttcagctttt
     5641 gcactgttcc cgctggtggc gattttctac cagtttggct ggaagcaaag tctgatcgcc
     5701 gccgtggtgg tactgatgac ccgtgtggta gtcgtgcgct atttcccaca tcttaaccct
     5761 gaatctatcg aaatctttat tggcatggtg atgctgctgg ggatcgcgat aactcacgac
     5821 ctgcgtcatc gtgatgaaaa tgacattgat gccagcgggc tttcggtgtt tgaagaacgc
     5881 acgtcacgga ttatcaaaaa cttaccctat atcgccatcg tgggagcatt gattgccgcc
     5941 gttgccagca tgaagatttt tgctggcagt gaagtgtcga tcttcacact ggagaaagca
     6001 tattccgcag gcgtaacgcc ggaacaatcg caaacgctga ttaatcaggc ggctctggca
     6061 gaatttatgc gcggactggg gtttgtgccg ttgattgcca ccaccgcgtt agcaacgggt
     6121 gtgtatgcag ttgcgggctt tacctttgtt tatgcggtgg gctatctctc gccgaatccg
     6181 atggttgcag cggtattagg tgcagtggtt atttcggcag aagttttatt gcttcgttcg
     6241 atcggcaaat ggctggggcg ctatccgtcg gtgcgtaatg cgtcggataa catccgtaac
     6301 gccatgaata tgctgatgga agtggcgctg ctggtcggtt cgattttcgc agcaattaag
     6361 atggcgggtt ataccggatt ctctatcgcg gttgccattt acttcctcaa cgaatccctg
     6421 ggccgtccgg tacagaaaat ggcggcaccg gtcgtggcag taatgatcac cggtattctg
     6481 ctgaatgttc tttactggct tggcctgttc gttccggctt aaggaaggca cctatgaaga
     6541 cgtttcctct gcaaagcctg acgattattg aggcgcagca aaagcagttt gcgctggtgg
     6601 atagcatttg tcgccatttc cccggcagcg agtttcttac tggcggtgat ttaggcttaa
     6661 cgccaggact gaatcaaccg cgcgttaccc agcgtgtgga acaggtgctg gctgatgcat
     6721 ttcacgcaca ggctgcggcg ctggtgcagg gcgcggggac tggcgcgatt cgcgccgggc
     6781 tggcggcttt gctcaaaccg gggcagcgtc ttctggtgca tgacgcgcct gtttacccga
     6841 cgacacgggt tattattgag cagatggggc tgacgcttat tactgttgat ttcaatgacc
     6901 tgtcggcact gaagcaggtc gtcgacgagc aacaaccgga tgcggcgctg gtgcagcata
     6961 cgcgccagca gccgcaggac agctacgtgc tggcagatgt gctggcaacg ttgcgcgcgg
     7021 caggtgttcc agcgttaacc gatgacaact atgcggtgat gaaggtggcg cgaatcggct
     7081 gtgaatgcgg cgcgaatgtc tcgacatttt cctgcttcaa gctatttggg ccagagggtg
     7141 ttggtgcagt ggtcggcgat gctgatgtta tcaaccgtat tcgcgccacg ctttactccg
     7201 gcggtagcca gatccagggc gcacaggcgc tggaagtatt gcgtggtctg gtgtttgcgc
     7261 cagtgatgca cgcggtgcag gcaggggtat ctgaacggtt gctggctttg cttaacggtg
     7321 gtgcggtgcc ggaagtgaaa agcgcggtga ttgctaatgc gcagtcgaag gtgttgattg
     7381 tcgagtttca tcagccgatt gccgccagag tgctggaaga ggcgcaaaag cgcggtgcct
     7441 tgccttaccc ggtgggtgca gagtcgaaat atgaaatccc gccgctcttt tatcgccttt
     7501 ccggaacgtt tcgccaggcg aatccacaat cagaacattg tgcgattcgc attaacccga
     7561 atcgcagcgg tgaagagacg gtgctgcgga ttttgcgtga gagtattgcc agtatttaat
     7621 cttgtgaatg atgttttcga aaagaatgca gaaacaaata cgccctcgga aaatccagag
     7681 ggcgtcgggc aattaaaccg gtgttagccg atttctgtca gagacttact gtgcagtagg
     7741 accgcaaccg ccaatgattt tagaaataga aattgccggg tgcagcaggt agtcgtaaga
     7801 gcagttctta tcacggtttt caatatgacc agtacatgca gttaacgtag ccagtactgc
     7861 acctaccagt gcaactttaa taaatttg
//
LOCUS       DAPHDW010000178         7877 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.181, whole genome shotgun sequence.
ACCESSION   DAPHDW010000178 DAPHDW010000000
VERSION     DAPHDW010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7877)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7877)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7877
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.181"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            105..194
                     /gene="kdpF"
                     /locus_tag="RH057_004035"
     CDS             105..194
                     /gene="kdpF"
                     /locus_tag="RH057_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HDV3130325.1"
                     /translation="MSAGVITGVLLVFLLLGYLVYALINAEAF"
     gene            194..1867
                     /gene="kdpA"
                     /locus_tag="RH057_004036"
     CDS             194..1867
                     /gene="kdpA"
                     /locus_tag="RH057_004036"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HDV3130326.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTTGVER
                     VLFRALGVSDREMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFALIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGALQNFQPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPGP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            1890..3938
                     /gene="kdpB"
                     /locus_tag="RH057_004037"
     CDS             1890..3938
                     /gene="kdpB"
                     /locus_tag="RH057_004037"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HDV3130327.1"
                     /translation="MSRKQLALFEPTLVVQALKEAVKKLNPQAQWRNPVMFIVWIGSL
                     LTTCISIAMASGAMPGNALFSAAISGWLWVTVLFANFAEALAEGRSKAQANSLKGVKK
                     TAFARKLREPKYGAAADKVPADQLRKGDIVLVEAGDIIPCDGEVIEGGASVDESAITG
                     ESAPVIRESGGDFASVTGGTRILSDWLVIECSVNPGETFLDRMIAMVEGAQRRKTPNE
                     IALTILLIALTIVFLLATATLWPFSAWGGNAVSVTVLVALLVCLIPTTIGGLLSAIGV
                     AGMSRMLGANVIATSGRAVEAAGDVDVLLLDKTGTITLGNRQASEFIPAQGVDEKTLA
                     DAAQLASLADETPEGRSIVILAKQRFNLRERDVQSLHATFVPFTAQSRMSGINIDNRM
                     IRKGSVDAIRRHVEANGGHFPTDVDQKVDQVARQGATPLVVVEGSRVLGVIALKDIVK
                     GGIKERFAQLRKMGIKTVMITGDNRLTAAAIAAEAGVDDFLAEATPEAKLALIRQYQA
                     EGRLVAMTGDGTNDAPALAQADVAVAMNSGTQAAKEAGNMVDLDSNPTKLIEVVHIGK
                     QMLMTRGSLTTFSIANDVAKYFAIIPAAFAATYPQLNALNIMRLHSPDSAILSAVIFN
                     ALIIVFLIPLALKGVSYKPLTASAMLRRNLWIYGLGGLLVPFIGIKVIDLLLTVCGLV
                     "
     gene            3947..4519
                     /gene="kdpC"
                     /locus_tag="RH057_004038"
     CDS             3947..4519
                     /gene="kdpC"
                     /locus_tag="RH057_004038"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HDV3130328.1"
                     /translation="MRGLRPALSTFIFLLLITGGVYPLLTTVLGQWWFPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFQGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAVWQIPRVAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            4512..7196
                     /gene="kdpD"
                     /locus_tag="RH057_004039"
     CDS             4512..7196
                     /gene="kdpD"
                     /locus_tag="RH057_004039"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
                     /protein_id="HDV3130329.1"
                     /translation="MNNEPLRPDPDRLLEQTAAPHRGKLKVFFGACAGVGKTWAMLAE
                     AQRLRAQGLDIVVGVVETHGRKDTAAMLEGLAVLPPKRQAYRGRHISEFDLDAALARR
                     PALILMDELAHSNAPGSRHPKRWQDIEELLEAGIDVFTTVNVQHLESLNDVVSGVTGI
                     QVRETVPDPFFDAADDVVLVDLPPDDLRQRLKEGKVYIAGQAERAIEHFFRKGNLIAL
                     RELALRRTADRVDEQMRAWRGHPGEEKVWHTRDAILLCIGHNTGSEKLVRAAARLASR
                     LGSVWHAVYVETPALHRLPEKKRRAILSALRLAQELGAETATLSDPAEEKAVVRYARE
                     HNLGKIILGRPASRRWWRRETFADRLARIAPDLDQVLVALDEPPARTINNAPDSRSFK
                     DKWRVQIQGCVVAAALCAVITLIAMQWLMAFDAANLVMLYLLGVVVVALFYGRWPSVV
                     ATVINVVSFDLFFIAPRGTLAVSDVQYLLTFAVMLTVGLVIGNLTAGVRYQARVARYR
                     EQRTRHLYEMSKALAVGRSPQDIAATSEQFIASTFHARSQVLLPDDNGKLQPLTHPQG
                     MTPWDDAIAQWSFDKGLPAGAGTDTLPGVPYQILPLKSGEKTYGLVVVEPGNLRQLMI
                     PEQQRLLETFTLLVANAFERLTLTASEEQARMASEREQIRNALLAALSHDLRTPLTVL
                     FGQAEILTLDLASEGSPHARQASEIRQHVLNTTRLVNNLLDMARIQSGGFNLKKEWLT
                     LEEVVGSALQMLEPGLSSPINLSLPEPLTLIHVDGPLFERVLINLLENAVKYAGAQAE
                     IGIDAHVEGENLQLDVWDNGPGLPPGQEQTIFDKFARGNKESAVPGVGLGLAICRAIV
                     DVHGGTITAFNRPEGGACFRVTLPQQTAPELEDFHEDM"
     gene            7193..7870
                     /gene="kdpE"
                     /locus_tag="RH057_004040"
     CDS             7193..7870
                     /gene="kdpE"
                     /locus_tag="RH057_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
                     /protein_id="HDV3130330.1"
                     /translation="MTNVLIVEDEQAIRRFLRTALEGDGMRVYEAETLQRGLLEAATR
                     KPDLIILDLGLPDGDGIEFIRDLRQWSAVPVIVLSARSEESDKIAALDAGADDYLSKP
                     FGIGELQARLRVALRRHSATAAPDPLVKFSDVTVDLAARVIHRGEEEVHLTPIEFRLL
                     AVLLNNAGKVLTQRQLLNQVWGPNAVEHSHYLRIYMGHLRQKLEQDPARPRHFITETG
                     IGYRFMP"
BASE COUNT         1591 a         2067 c         2360 g         1859 t
ORIGIN      
        1 ccatttttat acttttttta caccccgccc gcagattttt gcgaaatctt tgcagccaga
       61 attctaccct tccggtatca cttttaggcc actggaggtg cactgtgagt gcaggcgtga
      121 taaccggcgt attgctggtg tttttattac tgggttatct ggtttatgcc ctgatcaatg
      181 cggaggcgtt ctgatggctg cgcaagggtt cttactgatc gccacgtttt tactggtgtt
      241 aatggtgctg gcgcgtcctt taggcagcgg gctggcgcgg ctgattaatg acattcctct
      301 tcccggtaca acgggcgttg agcgcgtact ttttcgcgca cttggcgtct ctgaccgtga
      361 gatgaactgg aagcaatatc tttgtgccat tctcggcctg aacatgctgg ggctggcggt
      421 gctgtttttt atgttgctcg gtcagcacta tctgccgctt aatccacagc agttgccggg
      481 gctgtcgtgg gatctggcgc tgaataccgc cgtcagcttt gtcacgaata ccaactggca
      541 atcttacagc ggtgaaacca cgttgagcta tttcagccag atggctggct taacggtgca
      601 aaactttctt tctgccgcca gcgggattgc ggtgattttt gccctcatcc gtgcgtttac
      661 ccgccagagc atgagcacac tcgggaatgc ctgggtcgat ctgctacgca tcacgttatg
      721 ggtgctcgtc cctgtggcgt tgttgatcgc actgtttttt attcaacaag gtgcgctgca
      781 aaactttcag ccttatcagg ctgtgaatac cgttgaagga gcgcaacagc tgttacccat
      841 ggggcctgta gcttctcagg aagcgatcaa gatgctcggt actaacggcg gtggcttctt
      901 taatgccaac tcgtcgcatc cgtttgaaaa cccaaccgca ctgaccaact tcgtgcagat
      961 gctggcgatc ttcttgatcc caacggcgct gtgctttgcc tttggtgaag tgacgggcga
     1021 tcgccgccag ggacgcatgt tgctgtgggc gatgtcagtg atttttgtca tctgcgtagg
     1081 cgtggtgatg tgggcagaag ttcagggtaa tcctcatctg ctggcgctcg gcgcagacag
     1141 cagcatcaat atggaaggta aagagagccg tttcggcgtg ctggtcagta gcctgtttgc
     1201 ggtcgtgact acggcggctt cctgtggcgc ggtgattgcg atgcatgatt cgtttaccgc
     1261 tctcggtggc atggtgccga tgtggctgat gcaaattggt gaagtggtgt tcggcggtgt
     1321 cggttctggt ctttacggca tgatgctgtt tgtcctgctg gcggtgttta ttgccgggct
     1381 gatgattggt cgtacaccgg aatatctggg taaaaaaatc gacgtacgcg agatgaaact
     1441 gactgcactg gcgattctgg ttaccccgac gctggtgctg atgggcgcgg cgctggcaat
     1501 gatgaccgac gccggacgta gcgccatgct caaccctggc ccgcatggtt ttagcgaagt
     1561 gctgtacgcc gtgtcatccg ccgctaacaa caacggcagc gcctttgccg gattaagcgc
     1621 caactccccg ttctggaact gtttactggc gttctgcatg tttgtcggtc gcttcggggt
     1681 gattatcccg gtgatggcaa ttgccggttc gctggtgagt aaaaagagcc aacccgccag
     1741 ctccggcacg ctgccaacgc acggcccgct gtttgttggc ctgttaatcg gcaccgtgtt
     1801 gctggttggc gcactgacct ttatccctgc cctggcgctt ggtccggtgg cggaatatct
     1861 ctcctgatga tattgagtga gcactgaata tgagtcgtaa acaactggcg ctattcgaac
     1921 caacacttgt cgttcaggcg ctgaaagaag cggtgaaaaa attaaacccg caggcgcaat
     1981 ggcgcaatcc ggtgatgttt atcgtctgga tcggcagtct gctgaccacc tgtattagca
     2041 tcgcgatggc aagcggtgcg atgcctggca atgcgctgtt tagcgcggca attagcggct
     2101 ggctgtgggt caccgtactg ttcgctaatt tcgccgaggc gctggcagaa ggccgcagta
     2161 aagcgcaggc caacagtctt aaaggggtga aaaaaactgc ctttgcccgc aagctgcgtg
     2221 agccgaaata tggcgctgcg gcggacaaag ttcctgccga tcaactgcgt aaaggtgaca
     2281 tcgtgctggt agaagctggc gatattatcc cctgcgatgg tgaagtcatt gaaggcggcg
     2341 catcggtcga tgaaagcgcc atcaccgggg aatcggcacc ggtgatccgt gaatccggcg
     2401 gcgattttgc ctccgtcacc ggcggcacgc gtattctttc tgactggctg gtgattgagt
     2461 gtagcgttaa ccccggtgag acatttctgg atcggatgat cgcgatggtg gaaggcgcac
     2521 agcgacgcaa aacaccgaac gagattgccc tgactatcct gctgattgct ctgaccatcg
     2581 tctttttact ggcaaccgcc acgctgtggc cgttttccgc gtggggcggt aatgccgtga
     2641 gtgtaacggt actggtggcg ctgctggtct gtctgatccc aaccactatt ggcggcctgt
     2701 tgtcagcgat cggcgtcgcc gggatgagtc ggatgctggg cgcgaatgtg attgccacca
     2761 gcggacgtgc agttgaagcg gcaggtgacg ttgacgttct gctactggat aaaaccggca
     2821 ccatcacact cggtaaccgt caggcgtcgg agtttatccc tgcgcagggc gtggatgaaa
     2881 aaacgctggc tgacgccgca caactggctt cgctggctga tgaaacgccg gaaggccgca
     2941 gtattgtgat cctcgccaag caacgtttta acctgcgcga gcgcgatgtg cagtcgctcc
     3001 atgccacctt tgtaccgttt actgcgcaaa gccggatgag cgggatcaac atcgacaacc
     3061 gcatgatccg taaaggttct gtcgatgcca ttcgccgcca tgttgaggct aacggcggtc
     3121 acttccctac cgatgttgat caaaaagtcg atcaggttgc gcgtcagggg gccacgccgc
     3181 tggtggtggt ggaaggttcc cgtgtgctgg gcgttattgc gctgaaagat atcgtcaaag
     3241 gcggtattaa agagcgcttc gcccagctgc gcaaaatggg cattaaaacg gtgatgatta
     3301 ccggcgataa ccgtctgact gccgccgcca ttgctgcgga agcgggcgtc gatgattttc
     3361 tcgccgaagc gacaccggag gccaagctgg cattgattcg tcagtatcag gcggaaggcc
     3421 gtttggtggc gatgaccggc gacggcacca acgatgctcc agcgctggcg caggcagatg
     3481 tcgcggtggc gatgaactcc ggtactcagg cggcgaaaga agcgggcaat atggtcgatc
     3541 tcgactctaa cccgaccaag ctgatcgagg tggtacatat tggcaaacag atgctgatga
     3601 cccgtggctc gctgaccacc ttcagcattg ccaacgatgt ggcgaaatac ttcgccatta
     3661 ttccggcggc gttcgcggca acgtatccgc agttaaatgc gctgaacatc atgcgccttc
     3721 attcgcccga ctccgccatt ctcagtgcgg tcattttcaa cgccttgatt atcgtctttt
     3781 tgattcctct ggcgttaaaa ggcgtgagtt ataaaccgct taccgcttct gccatgttgc
     3841 gccgtaactt atggatttac ggtctgggtg ggctgctggt gccgtttatt ggtatcaaag
     3901 tcattgattt actgctgacc gtttgcggtc tggtgtgagg tttaccatgc gtggcttacg
     3961 tccggcatta tcgacattta tctttctgtt attgattact ggcggcgttt acccgctgct
     4021 gaccaccgtg ctggggcaat ggtggtttcc ctggcaggcc aacggttcgt tgattcgtga
     4081 aggtgatacg gtgcggggtt cggcattaat cgggcagaat tttaccggca acggctattt
     4141 tcagggtcgc ccgtcggcaa cggcagaaat gccctataat ccacaggctt ctggcgggag
     4201 caatctggcg gtcagtaacc ctgagctgga taaacaaata gccgcacgcg ttgctgcatt
     4261 acgggccgct aacccggatg ccagtacgaa cgttccggtt gaactggtga cggcatcggc
     4321 aagcgggctg gacaataata tcaccccgca agcggcggtc tggcaaatcc cacgcgtggc
     4381 gaaagcgcgt aatctcagcg ttgaacagct cacgcaactg atcgcaaaat acagccaaca
     4441 accgctggtg aaatatatcg gccagccggt cgtcaacatt gttgaactca atctggcgct
     4501 ggataaactt gatgaataac gaacccttac gtcccgaccc cgatcgtctg ctggaacaaa
     4561 ctgccgcgcc gcatcggggg aagctgaaag ttttcttcgg tgcctgtgca ggcgtcggga
     4621 agacctgggc gatgctggca gaagcccagc gactgcgggc gcaagggctg gatattgtgg
     4681 ttggcgtggt agaaacccat gggcggaaag ataccgccgc catgcttgaa gggctggctg
     4741 ttctgccgcc caaacgccag gcgtaccgtg ggcggcatat cagcgagttt gatctcgatg
     4801 ccgccctcgc ccgccgtccg gcgctgatct taatggacga actggcgcac agtaatgcgc
     4861 caggttctcg tcatcctaaa cgctggcagg atatcgaaga actgctggaa gctggcattg
     4921 atgttttcac aaccgtcaac gttcagcatc tggaaagtct gaatgatgtg gtcagcggcg
     4981 tcaccggaat tcaggtacgg gaaaccgtgc ccgatccttt tttcgatgcc gccgacgacg
     5041 tagtgctggt ggacttgccc ccggacgatc tgcgccagcg gctgaaagaa ggcaaagtct
     5101 atattgccgg gcaggcggag cgcgccattg aacatttttt ccgcaaaggc aatctgatcg
     5161 ccctgcgtga actggcactg cgccgtactg ccgatcgcgt agatgagcaa atgcgcgcct
     5221 ggcgggggca tcctggtgaa gagaaagtgt ggcacacgcg cgacgcgatc cttttatgca
     5281 tcgggcataa caccggcagt gagaaactgg tccgcgcggc ggcgcggttg gcctcgcgcc
     5341 tgggtagcgt ctggcacgcg gtgtatgttg aaacccctgc cctgcatcgc ttaccggaaa
     5401 aaaaacgtcg ggcaattctc agcgccttac gtctggcgca ggagctgggc gcggagacgg
     5461 ctacactttc tgatccagcg gaagaaaaag cagtagtgcg ttatgcccgt gaacataatc
     5521 tcggcaagat tattctcggt cgcccggcct cgcgccgctg gtggcgtcgg gaaacgtttg
     5581 ctgaccgact ggcgcgcatc gcccccgatc tcgatcaggt gctggtcgcg cttgatgaac
     5641 cacccgcccg cacgattaac aacgcgccgg atagccgctc ttttaaagac aagtggcggg
     5701 tacagattca gggatgcgtg gttgccgccg cgttatgcgc cgttatcact ttaattgcca
     5761 tgcagtggct gatggcgttt gatgccgcca acctggtgat gctgtatctg cttggcgtgg
     5821 tggtggtggc gctattttat ggacgctggc cttcggtggt tgctaccgtc attaatgtgg
     5881 tgagtttcga tctctttttt atcgccccac gcggcacgct cgctgtctct gatgtgcaat
     5941 atctgctgac cttcgcggtg atgttaaccg tcgggctggt gatcgggaac cttactgcgg
     6001 gtgtgcgtta tcaggcgcgg gtagcccgtt accgcgagca acgcacacgg cacttatatg
     6061 aaatgtcgaa agctctggcg gtgggccgca gtccgcagga catcgccgcc accagcgaac
     6121 aatttattgc ctccacgttt catgcccgca gtcaggtgtt gttgcccgat gacaacggta
     6181 aattgcagcc gttaacacat ccgcaaggaa tgacgccatg ggacgatgcc atcgcgcagt
     6241 ggagttttga taaaggcctg cctgcgggtg cgggcaccga cacgttaccc ggcgtgccgt
     6301 accagatttt gccgctaaaa agcggcgaga aaacctacgg actggtggtg gtggaaccgg
     6361 ggaatctgcg tcagttgatg atcccggagc agcagcgcct gctagagacg tttacgctgt
     6421 tagtcgccaa tgcgtttgag cggctgacgc tgaccgccag tgaagaacag gcgcggatgg
     6481 caagcgaacg tgaacagatc cgcaacgccc tgctggcggc gctttcgcat gatttacgca
     6541 cgccgcttac ggtgctgttt ggtcaggcag aaatcttaac gctcgatctg gcaagcgaag
     6601 gatcgcccca cgcccgccag gccagcgaga ttcgtcagca tgtgctgaac actacccgac
     6661 tggtgaataa tctactggat atggcgcgaa ttcagtccgg cggctttaat ttgaagaaag
     6721 agtggttaac gctggaagaa gtagtcggca gcgcgctgca aatgctggaa ccgggtttat
     6781 cgtcgcccat caatctttct ctgccagaac cgctgacctt aatccacgtt gacgggccac
     6841 tctttgaacg ggtgctgatt aatctgctgg agaacgcggt gaaatatgcg ggtgcgcagg
     6901 ccgaaattgg tatcgatgcc cacgttgagg gcgaaaatct acaactggat gtctgggata
     6961 acggccccgg tcttccgcca ggccaggagc agacgatatt tgataagttt gctcgcggga
     7021 ataaagagtc ggcagtaccg ggggtagggc ttggactggc aatttgtcgg gcgatagtgg
     7081 atgtacacgg gggcactatt accgcgttca accgaccgga aggtggtgcc tgttttcgtg
     7141 ttacacttcc ccagcaaacc gctcctgaac ttgaagactt tcatgaggat atgtgacaaa
     7201 cgttctgatt gttgaagatg aacaggctat tcgtcgcttt ctgcgcacgg cgctggaggg
     7261 cgacgggatg cgcgtctatg aggccgaaac gctgcaacgc ggcttgctgg aagcggctac
     7321 ccgtaagcca gatttgatta ttctcgatct cggcctgccc gatggtgatg ggattgagtt
     7381 tatccgcgac ctgcgccagt ggagcgcggt gccggtgatt gtgctttccg cacgcagcga
     7441 agagagcgac aaaatcgccg cgctggatgc cggagcggat gattatctga gtaagccgtt
     7501 tggcattggc gaattgcagg cccgtctgcg cgtcgcttta cgccgccact ccgccaccgc
     7561 cgcgcccgat ccgctggtga aattttccga tgttaccgtc gatttagccg cccgcgtgat
     7621 tcaccggggt gaggaagagg tgcatctcac gccgattgag ttccgcctgc tggcggtgct
     7681 gctgaataat gccgggaaag tgctcaccca gcgccagtta ctcaatcagg tgtgggggcc
     7741 aaacgcggtc gaacacagtc actatttgcg tatttatatg ggacatctgc gacaaaaact
     7801 ggaacaggat cccgctcgcc cccgccattt cattactgaa accggtattg ggtatcggtt
     7861 tatgccatga atgttta
//
LOCUS       DAPHDW010000179         7868 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.182, whole genome shotgun sequence.
ACCESSION   DAPHDW010000179 DAPHDW010000000
VERSION     DAPHDW010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.182"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1540
                     /gene="iucA"
                     /locus_tag="RH057_004041"
     CDS             <1..1540
                     /gene="iucA"
                     /locus_tag="RH057_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HDV3130331.1"
                     /translation="FAFPAHLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLCETFH
                     QRVLESYAHTQQTIDARHDWAILREKALNFGEAEQALLTGHAFHPAPKSHEPFNRQEA
                     ERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDNQWLF
                     PLHPWQGEYLLQQVWCQALFAKGLIRDLGEAGTSWLPTTSSRSLYCATSRDMIKFSLS
                     VRLTNSVRTLSVKEVERGMRLARLAQTDGWQMLQARFPTFRVMQEDGWAGLRDLNGNI
                     MQESLFSLRENLLLEQPQSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITVQQAA
                     HAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSAFMPH
                     ATEWLDTIDEAQAENIFTREQLLRYFPYYLLVNSTFAVTAALGAAGLDSEANLMARVR
                     TLLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFANPLQ
                     AQEV"
     gene            1541..2488
                     /gene="iucB"
                     /locus_tag="RH057_004042"
     CDS             1541..2488
                     /gene="iucB"
                     /locus_tag="RH057_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HDV3130332.1"
                     /translation="MSGANIVHSGYGLRCEKLDKPLNLGWGLDNSAVLHWPGELPTGW
                     LCDALDQIFIAAPQLSAVVLPWSEWCEEPQALTLFGQVQSDIIHRSAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQIAYLERQLTSKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGVTHYLLLNEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            2488..4230
                     /gene="iucC"
                     /locus_tag="RH057_004043"
     CDS             2488..4230
                     /gene="iucC"
                     /locus_tag="RH057_004043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HDV3130333.1"
                     /translation="MNHKDWDLVNRRLVAKMLSELEYEQVFHAESQGDDRYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCADEPVLAQTLLMQLKQVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLNADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     ANTFRLHWLAVKREHMIWRCDNEMDIHQLLTAAMDPQEFARFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEEFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMKKHPQMSERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLED
                     LQNPLWLVTQEYES"
     gene            4227..5504
                     /gene="iucD"
                     /locus_tag="RH057_004044"
     CDS             4227..5504
                     /gene="iucD"
                     /locus_tag="RH057_004044"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001354579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HDV3130334.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELDCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKRRLFLVQTSQGEYFARNICLGTGKQPYLPPCVK
                     HMTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLEEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWKLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFVVNASMQTHGIAEPQLSL
                     MAWRSARILNRVMGRDLFDLSMPPALIQWRSGT"
     gene            5586..7787
                     /gene="iutA"
                     /locus_tag="RH057_004045"
     CDS             5586..7787
                     /gene="iutA"
                     /locus_tag="RH057_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HDV3130335.1"
                     /translation="MMISKKYTLWALNPLLLTMMAPAVAQQTDDETFVVSANRSNRTV
                     AEMAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSRSRTNYGMNVRGRPLVVL
                     VDGVRLNSSRTDSRQLDSIDPFNIDHIEVISGATSLYGGGSTGGLINIVTKKGQPETM
                     MEFEAGTKSGFSSSKDHDERIAGAVSGGNEHISGRLSVAYQKFGGWFDGNGDATLLDN
                     TQTGLQYSDRLDIMGTGTLNIDESRQLQLITQYYKSQGDDDYGLNLGKGFSAIRGTST
                     PFVSNGLNSDRIPGTERHLISLQYSDSAFLGQELVGQVYYRDESLRFYPFPTVNANKQ
                     VTAFSSSQQDTDQYGMKLTLNSKPMDGWQITWGLDADHERFTSNQMFFDLAQASASGG
                     LNNKKIYTTGRYPSYDITNLAAFLQSGYDINNLFTLNGGVRYQYTENKIDDFIGYAQQ
                     RQIAAGKATSADAIPGGSVDYDNFLFNAGLLMHITERQQAWLNFSQGVELPDPGKYYG
                     RGIYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFTGNNLRTQIAAYYSISDKS
                     VVANKDLTISVVDDKRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGTWQKYDV
                     KTASPSKATAYIGWAPDPWSLRVQSTTSFDVSDAQGYKVDGYTTVDLLGSYQLPVGTL
                     SFSIENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
BASE COUNT         1819 a         2078 c         2116 g         1855 t
ORIGIN      
        1 ctttgcattt cctgctcatc tggtcacggc atcaggcagt tatcctgtcg actttaccac
       61 tctctcccgg cttattattg ataagctccg gcatcaactt tttctgccag ttcccctctg
      121 cgaaactttc caccaacgcg tgctggaaag ctacgcccat acgcaacaga caattgatgc
      181 ccgccatgac tgggccatcc tgcgtgaaaa agcgttgaat tttggcgagg ctgagcaggc
      241 actgctgaca ggacacgctt tccaccctgc acctaagtct catgaaccgt ttaaccgcca
      301 ggaagctgaa cgatacctgc ctgacatggc acctcacttc ccgctgcggt ggttttcggt
      361 ggataaaacg caaatcgctg gtgaaagtct gcatcttaac cttcaacagc ggttgacgcg
      421 atttgccgca gaaaatgcgc cccagttact caacgaatta agtgacaatc aatggctgtt
      481 cccgctgcac ccgtggcagg gagaatatct tttgcagcaa gtgtggtgcc aggcactttt
      541 tgctaaagga cttatcagag acttaggcga ggccggcacg tcgtggctgc cgaccacctc
      601 ttcccgctcc ctctactgtg ctaccagccg cgatatgatc aagttctccc tgagcgttcg
      661 gctgaccaac tccgtccgta ctctgtctgt gaaagaggtg gagcgaggaa tgcgcctggc
      721 acgtctggcg caaaccgacg gctggcagat gctacaggcc cgcttcccta ctttccgggt
      781 aatgcaggag gacggctggg ccgggctgcg cgatcttaac ggcaacatca tgcaggaaag
      841 tttgttttcc ctgcgtgaaa acctgctgct ggagcagccg caaagccaga ccaacgttct
      901 ggtttccctg actcaggccg caccggatgg cggtgattcg ctgctggttt cggcggtaaa
      961 acgcctgagc gatcgcctcg gtatcactgt gcaacaggcc gcccacgcat gggtcgatgc
     1021 ttactgtcag caggtgctga agcctctgtt tacggctgaa gcggattacg gcctggtgct
     1081 gctggcgcat cagcaaaata ttcttgtgca gatgcttggg gatctgccgg tcggatttat
     1141 ttaccgtgac tgccagggca gcgcgtttat gcctcacgcg acagagtggc tcgataccat
     1201 tgacgaggcg caggcggaaa atattttcac ccgtgagcag ttgctgcgct atttccctta
     1261 ttacctgctg gttaactcca cttttgccgt aactgctgcg ctgggtgctg ccgggctgga
     1321 cagcgaagcg aatctgatgg ctcgtgtacg aacattgctg gctgaagtgc gtgaccaggt
     1381 gactcataaa acctgtctca actatgttct ggaaagtccg tactggaacg taaaaggtaa
     1441 ctttttctgt tatctgaacg atcataacga gaacaccatc gttgaccctt cggtgatcta
     1501 ttttgatttc gctaacccgc tgcaggctca ggaggtctga atgtctgggg caaacattgt
     1561 tcacagcgga tatggactgc gctgtgaaaa actcgacaag cctctgaatc ttggctgggg
     1621 gctggacaat agcgcggtgt tgcactggcc cggggagctg ccaacagggt ggctgtgcga
     1681 cgcgctggat cagatattta tcgccgcacc acaactttca gcagtggttc ttccctggtc
     1741 cgaatggtgt gaggagccac aggcgctgac gcttttcgga caggtacaaa gcgacattat
     1801 ccatcgttcc gctttctggc agttaccgtt atggctgagt tctccggcaa accgggcctc
     1861 cggtgaaatg gtttttgatg cagagcgtga gatttatttc ccgcagcgcc ccccccgtcc
     1921 gcagggtgaa gtttatcgtc gttacgatcc acgcattcgc aggatgctga gtttccgcat
     1981 tgccgatccc gtttctgatg cagaacgttt cactcgctgg atgaacgatc cgcgcgttga
     2041 gtatttctgg gagcaaagtg gctcactgga ggtacagatc gcctatctgg aacgccagct
     2101 caccagtaaa catgcattcc cgctgattgg ttgtttcgac gatcggccgt tcagttattt
     2161 cgaaatctac tgggcggcgg aagaccgcat tggtcgccac tattcgtggc agccctttga
     2221 ccgtggcctg catctgctgg ttggtgaaca gcaatggcgc ggagcccact atgtgcaaag
     2281 ctggctgcgc ggggtgacac attacctgct gctgaatgag ccacgtacgc agcgcacagt
     2341 actggagcca cgcaccgata accagcgcct gttccgccat cttgagcctg cgggataccg
     2401 gacaattaaa gagttcgact tcccacataa acgctcgcgc atggtgatgg cggatcgtca
     2461 tcacttcttc acggaggtcg gtctgtaatg aatcacaaag actgggattt ggtcaaccgc
     2521 cgcctggtgg caaaaatgtt gtctgagctg gagtatgagc aggttttcca cgcggaatct
     2581 cagggcgatg accgctactg cattaacctg ccgggagcac aatggcgctt catcgctgaa
     2641 cgtggtatct ggggctggct ctggattgat gctcaaactc tgcgctgcgc ggacgagcca
     2701 gtactggctc agacgctgct gatgcagcta aagcaggtac tgtcaatgag cgatgcaacc
     2761 gttgctgagc atatgcagga tttgtatgcc acgctgctgg gcgacctgca actactgaaa
     2821 gcccgtcgcg ggctgagcgc cagtgacctg attaatctta atgccgaccg cctgcaatgc
     2881 ctgctgagcg gtcatcctaa attcgttttt aataaaggtc gccgtggctg gggtaaagag
     2941 gcgctggaac gatatgcgcc agagtatgcc aacaccttca gactgcactg gctggcggta
     3001 aaacgtgaac atatgatctg gcgctgtgat aacgagatgg atattcatca gttgttgacg
     3061 gccgcaatgg atccgcagga gtttgcccgc ttcagtcagg tctggcagga aaacggactg
     3121 gatcataact ggctgccgct gccggtacat ccgtggcagt ggcagcaaaa aatcgctacc
     3181 gacttcatcg ctgattttgc cgaaggcagg atggtgtctc tcggcgagtt tggcgaccag
     3241 tggctcgccc agcagtcgct gcgtaccctg accaacgcca gtcgccgggg cgggctggat
     3301 atcaagctgc cgctgaccat ctacaacacc tcatgctacc gggggatccc tggcagatac
     3361 atcgctgccg gaccactggc ttcacgctgg ctacaacagg tttttgcgac cgacgccacc
     3421 ctagtgcaaa gcggcgcagt gatacttggt gaaccggctg caggctatgt ctcccatgaa
     3481 ggctatgccg cgcttgcccg ggctccctat cgctaccagg aaatgcttgg tgttatctgg
     3541 cgggagaatc cgtgccgctg gctgaaaccg gatgaaagtc cggttctgat ggcaacactg
     3601 atggagtgcg acgaaaacaa tcagccgctg gcaggcgcat atatagaccg ctccgggctg
     3661 gacgctgaaa cctggcttac gcaactgttc cgggtggtcg tggttcctct gtatcacctg
     3721 ctttgccgct acggtgtcgc gcttattgca catggacaaa atataactct cgccatgaaa
     3781 gagggggttc cacagcgtgt tctgctgaaa gacttccagg gcgatatgcg actggtgaaa
     3841 gaagagttcc ccgaaatgga ctctttgcct caggaggttc gtgatgttac atcccgcctg
     3901 agtgcggact acttaatcca tgatttgcag acgggtcact tcgtgacagt actgcgtttt
     3961 atttcgccac tgatggttcg tcttggcgta cctgaaaggc gattttatca actgctggca
     4021 gcagtgttga gtgattacat gaaaaaacat ccacaaatgt cagagcgttt tgcgcttttc
     4081 tcacttttca ggccacaaat cattcgcgtg gtgctgaacc cggtaaaact gacctggcct
     4141 gatctggatg gcggcagccg catgctgccg aattaccttg aggatctgca aaatccgctg
     4201 tggctggtaa ctcaggaata tgaatcatga aaaaaagtgt cgattttatt ggtgtaggga
     4261 cagggccatt taatctcagc attgctgcgt tgtcacatca gatcgaagaa ctggactgtc
     4321 tcttctttga tgaacatcct catttttcct ggcatccggg tatgctggta ccggattgtc
     4381 atatgcagac cgtctttctg aaagatctgg tcagtgctgt tgcacctaca aatccctaca
     4441 gttttgttaa ctatctggtg aagcacaaaa agttctatcg cttccttaca agcagactac
     4501 gtacagtatc ccgtgaagag ttttctgact acctccgctg ggctgctgaa gatatgaata
     4561 acctgtattt cagtcatacc gttgaaaaca ttgatttcga taaaaaacgt cgattgtttc
     4621 tggtgcaaac cagccaggga gaatattttg cccgcaatat ctgccttggt acaggaaaac
     4681 aaccttattt accaccctgt gtgaagcata tgacacaatc ctgtttccat gccagtgaaa
     4741 tgaatcttcg tcggccggac cttagtggaa aacggataac cgtggttggt ggaggacaga
     4801 gtggtgcaga cctgttcctt aatgcattac gcggggaatg gggagaagcg gcggaaataa
     4861 actgggtctc ccggcgtaat aattttaacg cactggatga ggctgctttt gctgatgagt
     4921 attttacacc tgaatatatt tcaggcttct ccggactgga ggaagatatt cgccatcagt
     4981 tactggatga gcagaaaatg acatcggatg gcatcactgc cgattcttta ctgaccattt
     5041 atcgtgagtt gtaccaccgt tttgaagttc tgagaaaacc aagaaatatc cgtctgctac
     5101 ccagccgctc ggtaacaact ctggaaagta gtggtcctgg ctggaagttg ctgatggagc
     5161 atcatctgga tcagggcagg gagagcctgg aaagtgatgt ggtgattttc gccacaggtt
     5221 accgttctgc gttgccacaa atacttccct cactgatgcc cctgatcacc atgcacgata
     5281 agaacacctt taaagtgcgt gatgacttca ctctggaatg gagtggcccg aaagagaaca
     5341 acatcttcgt ggtcaacgcc agtatgcaaa cccatggcat cgccgaaccc cagctcagcc
     5401 tgatggcatg gagatctgca cgtattctta atcgcgtaat gggacgtgat ttattcgatc
     5461 tcagtatgcc gcccgccctg attcagtggc gcagcggcac ctagggaaaa cgcagccgga
     5521 ggctgcttct ttaactcgct acacagcatc tttgggctga ttttttccgc ccgtatggag
     5581 gaataatgat gataagcaaa aagtatacgc tttgggctct caacccactg cttcttacca
     5641 tgatggcgcc agcagtcgct caacaaaccg atgatgaaac gttcgtggtg tctgccaacc
     5701 gcagcaatcg caccgtagcg gagatggcgc aaaccacctg ggttatcgaa aacgccgaac
     5761 tggaacagca gattcagggc ggcaaagagc ttaaagacgc actggctcag ctgatccctg
     5821 gccttgacgt cagcagccgg agccgcacca actacggtat gaatgtgcgt ggccgcccgc
     5881 tggtcgtgct ggttgacggc gtgcgtctca actcttcacg taccgacagc cgacaactgg
     5941 actctataga tccttttaat atcgaccata ttgaagtgat ctccggtgcg acgtccctgt
     6001 acggcggcgg cagtaccggt ggcctgatca acatcgtgac caaaaaaggc cagccggaaa
     6061 ccatgatgga gtttgaggct ggcaccaaaa gtggctttag cagcagtaaa gatcacgatg
     6121 aacgcattgc cggagctgtc tccggcggaa atgagcatat ctccggacgt ctttccgtgg
     6181 catatcagaa atttggcggc tggtttgacg gtaacggcga tgccaccttg cttgataaca
     6241 cccagaccgg cctgcagtac tccgatcggc tggacatcat gggaactggt acgctgaaca
     6301 tcgatgaatc ccggcagctt cagttgatca cacagtacta taaaagccag ggcgacgacg
     6361 attacgggct taatctcggg aaaggcttct ctgccatcag agggaccagc acgccattcg
     6421 tcagtaacgg gctgaattcc gaccgtattc ccggcactga gcggcatttg atcagcctgc
     6481 agtactctga cagcgctttt ctgggacagg agctggtcgg tcaggtttac taccgcgatg
     6541 agtcgttgcg attctacccg ttcccgacgg taaatgcgaa caaacaggtg acggctttct
     6601 cttcgtcaca gcaggacacc gaccagtacg gcatgaaact gactctgaac agcaaaccga
     6661 tggacggctg gcaaatcacc tgggggctgg atgctgatca tgagcgcttt acctccaacc
     6721 agatgttctt cgacctggct caggcaagcg cttccggagg gctgaacaac aagaagattt
     6781 acaccaccgg gcgctatccg tcgtatgaca tcaccaacct ggcggccttc ctgcaatcag
     6841 gctatgacat caataatctc tttaccctca acggtggcgt acgctatcag tacactgaaa
     6901 acaagattga tgatttcatc ggctacgcgc agcaacggca gattgccgcc gggaaggcta
     6961 catccgccga cgccattcct ggcggctcag tcgattacga caacttcctg ttcaacgccg
     7021 gtctgctgat gcacatcacc gaacgccagc aggcatggct caacttctcc cagggcgtgg
     7081 agctgccgga cccgggtaaa tactatggtc gcggcatcta tggtgctgca gtgaacggcc
     7141 atcttcctct aacaaagagt gtgaacgtca gcgacagcaa gctggaaggc gtgaaagtcg
     7201 attcttatga gctgggctgg cgctttactg gcaataatct gcgtacccaa atcgcggcct
     7261 actattcgat ttctgataag agcgtggtgg cgaataaaga tctgaccatc agcgtggtgg
     7321 acgacaaacg ccgtatttac ggcgtggaag gtgcggtgga ctacctgatt cctgatactg
     7381 actggagtac cggagtgaac ttcaacgtgc tgaaaactga gtcgaaagtg aacggtacct
     7441 ggcagaaata cgatgtgaag acagcaagcc catcaaaagc gacagcctac attggctggg
     7501 caccggaccc gtggagtctg cgcgtgcaga gcaccacctc ctttgacgtg agcgacgcgc
     7561 agggctacaa ggtcgatggc tataccaccg tggatctgct cggcagttat cagcttccgg
     7621 tgggtacact cagcttcagc attgaaaacc tcttcgaccg tgactacacc actgtctggg
     7681 ggcagcgtgc accactgtac tacagcccgg gttacggccc agcgtcactg tacgactaca
     7741 aaggcagggg ccgaaccttt ggtctgaact actctgtgct gttctgaccg gtattccttt
     7801 acaacaaagg tacgctgata tcaacatggc cgctgacagc caagttgata tcatataata
     7861 cacgacat
//
LOCUS       DAPHDW010000180         7867 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.183, whole genome shotgun sequence.
ACCESSION   DAPHDW010000180 DAPHDW010000000
VERSION     DAPHDW010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7867
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.183"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(412..1160)
                     /gene="envY"
                     /locus_tag="RH057_004046"
                     /pseudo
     CDS             complement(412..1160)
                     /gene="envY"
                     /locus_tag="RH057_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308625.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
     gene            complement(1343..2233)
                     /locus_tag="RH057_004047"
     CDS             complement(1343..2233)
                     /locus_tag="RH057_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HDV3130336.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRLQGFDTLVLQWTRYGDAFTQPEQRALLFKRAAAAQQAGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGVTPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSPAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDTLYFSLRYLPVAHGILEY"
     gene            complement(2234..5206)
                     /gene="nfrA"
                     /locus_tag="RH057_004048"
     CDS             complement(2234..5206)
                     /gene="nfrA"
                     /locus_tag="RH057_004048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
                     /protein_id="HDV3130337.1"
                     /translation="MKENNLNRVIGWSGLLLTSLLSTSALADNIGTSAEELGLSDYRH
                     FVIYPRLDKALKAQKNNDEATAIREFEYIHQQVPDNIPLTLYLAEAYRHFGHDDRARL
                     LLEDQLKRHPGDARLERSLAAIPVEVKSVTTVEELLAQQKACDAAPTLRCRSEVGQNA
                     LRLAQLPVARAQLNDATFAASPEGKTLRTDLLQRAIYLKQWSQADTLYNEARQQNTLS
                     AAERRQWFDVLLAGQLDDRILALQSQGIFTDPQSYITYATALAYRGEKARLQHYLNEN
                     KPLFTTDAQEKSWLYLLSKYSANPVQALANYTVQFADNRQYVVGATLPVLLKEGQYDA
                     AQKLLATLPANEMLEERYAVSVATRNKAESLRLARLLYQQEPANLTRLDQLTWQLMQN
                     EQSREAADLLLQRYPFQGDARVSQTLMARLASLLESHPYLATPAKVAILSKPLPLAEQ
                     RQWQSQLPGIADNCPAIVRLLGDMSPSYDAAAWNRLAKCYRDTLPGVALYAWLQAEQR
                     QPNAWQHRAVAYQAYQVEDYATALAAWQKISLHDMSNEDLLAAANTAQAAGNGAARDR
                     WLQQAEQRGLGNNALYWWLHAQRYIPGQPELALSDLTRSINIAPSANAYVARATIYRQ
                     RHNVPAAVSDLRAALELEPNNSNIQAALGYALWDSGDIAQSREMLEQAHKGLPDDPAL
                     IRQLAYVNQRLDDMPATQHYARLVIDDIDNQALITPLTPEQNQQRFNFRRLHEEVGRR
                     WTFSFDSSIGLRSGAMSTANNNVGGAAPGKSYRSYGQLEAEYRIGRNMLLEGDLLSVY
                     SRVFADTGENGVMMPVKNPMSGTGLRWKPLRDQIFFLAVEQQLPLNGQNGASDTMLRA
                     SASFFNGGKYSDEWHPNGSGWFAQNLYLDAAQYIRQDIQAWTADYRVSWHQKVANGQT
                     IEPYAHVQDNGYRDKGTQGAQLGGVGVRWNIWTGETHYDAWPHKVSLGVEYQHTFKAI
                     NQRNGERNNAFLTIGVHW"
     gene            complement(5193..7430)
                     /gene="nrfB"
                     /locus_tag="RH057_004049"
     CDS             complement(5193..7430)
                     /gene="nrfB"
                     /locus_tag="RH057_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HDV3130338.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGDGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMLQLLLLLAYESLWPNAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRALKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            complement(7699..>7867)
                     /locus_tag="RH057_004050"
     CDS             complement(7699..>7867)
                     /locus_tag="RH057_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130339.1"
                     /translation="IRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAMDRNY
                     LASVLAGSGLS"
BASE COUNT         1832 a         2008 c         2059 g         1968 t
ORIGIN      
        1 tattgaatga ttatcacaat gcgctatctg tttttggttt aattatctgt tattgtttta
       61 tgttgcggtt ttactgtgtg gttttttatg cttttgtggt gtttttatct atttaagcgc
      121 catgccttta aaggcatata agcgaaaata gcatgaggtt tatcctcaat tgctatgttt
      181 tttagtacaa aaaagaggga caaaactgag acacataagg cctcacaatg gcttgcaagg
      241 ctttacatgt tttgaggtag tgggacgtgt gagcgcagag atggcgcggt aatttgttga
      301 cttaaaatgt cgttctagga acttctaagt cgtgggccgc aggttcgaat cctgcagggc
      361 gcgccatttc ttcttcattt atgcccgtct tatccgtttc cgctttgccc ttcaccacat
      421 cactttttgt cgctgtttgg cgagataatt caacggtgtc aggccgtaaa acgccttaaa
      481 aacagagata aagtatgacg tgctgctata gccacataat tgcgccacct gagtgatatt
      541 tttgttatcc atcaataaca tctgtacggc gtaacgcata cgacactctg tgacaatctg
      601 gctatagctg gtattttcgt tttttaattt ctttttgagc aggctggggc ttaaacatag
      661 cgaactggcg acaattcgca gattccagta atgctgaata tcgctttgaa taatgcggca
      721 gacgctgtcg cggacgctgc tgcgtaagat atacatcagt agggctataa accgcgattg
      781 ctcaagaaag ttagacaata cggtaaaaag taatgcgcgt gtcaactccg cttcgcaggg
      841 tttgctgggt tgctgcttgc tgtggttggc ggctagccgg aacacttccg gtgtacggct
      901 atgacaagcg ataaccgggg ttgccagccg cgaccagggc tttacacagg taaaaacaac
      961 agataatcgt tgatgatgtt acggttgatg tgggggatta atacgtgatt taacgttgag
     1021 agttcaatga cgttattgtt gcacgcgaag gcggccaggt agtttttcgg aagggtaaac
     1081 gtagcatggt tattgacgct tacctctacc tctttttcgg tcaggatcac cacgcaaggt
     1141 tcactgctgc tcaattgcat ttcgcactcc tcagatatca gaaactccgc tcaaaggatc
     1201 tatgcttcct gcatgagtga tcggcccgtt cgccgataac gatcttcttt ctttagcacg
     1261 ctttttagca attaatcttg atggaattct gacgagagcg aaagaggtaa gccaggtcgt
     1321 acccgactta cctggaggag atttaatact cgagaatgcc gtgcgcgacg ggcaaatagc
     1381 gcagagagaa atagagagtg tctttaccgc aagaggaacg tttcgctaac agcgaggcga
     1441 tctctgcatc cggtttcggt ttcgctgtaa aggttttgcc tttgccggcg acaaacagtt
     1501 cataaacaat gccgctggca ggggcgggac tttggcaatc ggcgctggcc tgtagataac
     1561 gttcacgctg ttcagcggta agtttatcca cgccgctgcc atcctgtacc cagacattaa
     1621 cgccagttgc tttaacttgt tccagcagtt ggcgatagcc atcgggcgac atgtttccgg
     1681 cgaaaaaact actgatataa accggttttg ctgaaacatc gctaatcagc cgctgcgcgt
     1741 tgtttaacca tgttagcaaa ggctgacggg cagcttcgct gcgccagttc aggtcgtcaa
     1801 tttccgcgct gatgtaccag ccatccggcg ttacgccagg cgcggcgctc cataatctgg
     1861 cttgctggag atcggcagcc agcaggcgat taagatagct ttccagcgct gcggacgact
     1921 gtttctggtg cataaaaaat tccggatcgg cgttcagccc gacaatcagc ttcaggccag
     1981 cctgttgcgc agctgcggca cgcttaaaca ataaagcgcg ctgttctggc tgggtgaatg
     2041 catcgccgta acgggtccat tgcaaaacca gggtatcgaa gccttgcaaa cgtaactgac
     2101 tcatcagccc ctgccactgg gtatcggtaa cctgactatc tcggttttgt ggttgccaga
     2161 taataccttt catcgcaaag gaaaaagggc tgaccaaaag cagtgtcagc aatacgaaaa
     2221 tgaacttacg catttaccag tgcactccaa tggtgagaaa cgcgttgttg cgctctccgt
     2281 tacgttgatt aatcgcctta aaggtatgtt gatactcgac gccgagactg actttgtgcg
     2341 gccaggcgtc gtagtgcgtc tcgccggtcc agatattcca gcggaccccg actccgccaa
     2401 gctgcgcgcc ctgagtgcct ttatcacgat agccgttgtc ctgaacgtga gcgtaaggct
     2461 caatagtctg tccgttagct accttctgat gccagctgac gcgataatct gccgtccacg
     2521 cctgaatatc ctggcggata tattgcgccg catcgaggta caggttttgg gcaaaccagc
     2581 ctgaaccgtt cgggtgccat tcgtcgctgt atttgccgcc attaaagaat gaggcgctgg
     2641 cgcgcagcat ggtatcggat gcgccatttt gcccgttcag cggcaactgc tgttcgacgg
     2701 cgaggaaaaa gatctgatcg cgcagcggct tccagcgcag accggtgccg gacatcggat
     2761 ttttcaccgg catcatcacc ccgttttctc cggtatcggc aaagacgcgg ctataaactg
     2821 agagcaggtc gccttccagc agcatattgc gtccgatgcg gtactcggct tccagttgcc
     2881 cgtagctacg atagcttttc cctggcgctg cgccgccgac attattgtta gcggtgctca
     2941 ttgccccgga acgcaagccg atagaagaat cgaaactgaa cgtccagcgg cgaccgacct
     3001 cctcatgcaa acggcggaaa ttgaagcgtt gctgattttg ctctggggtc agtggggtta
     3061 tcagcgcctg attatcaatg tcatcaatca ccagccgggc gtaatgctgc gtcgcaggca
     3121 tgtcatccag acgctggttc acataggcca gttgtcggat cagtgccgga tcgtccggaa
     3181 gccctttatg cgcctgttcg agcatttccc gcgactgtgc gatatcaccg ctatcccaca
     3241 aggcgtaacc aagcgctgcc tggatgttgc tattattcgg ttccagttcc agcgcggcgc
     3301 gcaaatcact caccgccgcc gggacattat gacgttggcg ataaattgtc gcccgcgcaa
     3361 cgtaagcgtt ggcagaaggc gcaatattga ttgagcgcgt gagatcgctc agtgcgagtt
     3421 ccggctgacc aggaatgtaa cgttgcgcat gcagccacca gtagagggca ttgtttccca
     3481 gtccacgttg ttctgcctgt tgtagccagc gatcgcgagc tgcaccattt cctgccgcct
     3541 gggcggtatt ggcagcagca agcagatcct cattgctcat gtcgtgaaga ctgattttct
     3601 gccaggccgc cagtgcggtg gcgtagtcct caacctgata cgcctgatag gctaccgcac
     3661 gatgttgcca ggcgttcggt tgtcgttgtt cggcctgaag ccatgcatac agcgccacac
     3721 cgggtagcgt gtcccgataa cactttgcca gacggttcca ggcggcagca tcgtaggaag
     3781 gcgacatatc gcccagcaag cgaactattg ccgggcaatt atctgcaata cccggcaact
     3841 gactttgcca ctgacgttgc tccgccagcg gtaagggttt cgataaaatc gccaccttcg
     3901 ccggcgttgc caggtaagga tgactttcca gcagagacgc cagtcgcgcc attaaagtct
     3961 ggctgacacg cgcatcgccc tggaaaggat agcgttgcag caataaatcg gcagcttcgc
     4021 gtgactgctc gttctgcatc agttgccagg tcagttgatc caggcgggta agatttgccg
     4081 gttcttgctg atacagcaat cgtgccagac gcagagattc agccttgtta cgggtcgcca
     4141 cgctgacagc ataacgctcc tcaagcattt cattggcggg gagggtggcg agcagttttt
     4201 gcgctgcgtc gtactgacct tcttttaaca gcaccggtag cgtcgcgcca acaacatact
     4261 ggcggttatc ggcaaactgc accgtataat tcgccaatgc ctgaacgggg ttggcgctgt
     4321 atttagataa cagatagagc caacttttct cttgtgcgtc cgtggtaaac agcggcttat
     4381 tttcattgag ataatgctgg aggcgtgctt tttcgccacg ataagccagc gcggtcgcgt
     4441 aagtaatata tgactgagga tcggtgaaga tcccctgtga ttgcagtgcc aggatccgat
     4501 cgtccagctg cccggcaaga agcacgtcaa accactgacg gcgttctgcc gcgcttaatg
     4561 tgttctgctg gcgtgcttca ttgtatagcg tatctgcctg ggaccattgt ttcaggtaga
     4621 ttgcccgttg cagcagatcg gttcgcagcg tttttccttc tggcgatgca gcaaacgtcg
     4681 catcgttcag ttgcgctctg gcgacaggta attgtgccag ccgcagggca ttctgcccga
     4741 cttcactgcg acaacgcagg gtcggcgcag cgtcgcacgc tttttgctgg gcaagcagtt
     4801 cttcaacagt cgtaacgctt ttcacttcaa ccggaatagc cgccagactg cgctcaagtc
     4861 gggcatctcc tgggtgacgt ttcagttgat cctcaagcaa cagccgcgcc cggtcatcat
     4921 gaccaaaatg gcgataggct tccgcaaggt ataaagtcag cggaatatta tccggcacct
     4981 gctggtgtat atattcaaat tcgcggatgg cggttgcttc gtcgttattt ttctgtgcct
     5041 tcagcgcctt atcgagacgg ggataaataa caaaatggcg ataatcgctc agccccagct
     5101 cttctgcgct ggtgccgata ttgtctgcga gtgcgctggt actcaataaa gacgtcagca
     5161 gtaaaccaga ccatccgatg acgcgattaa ggttattctc cttcattttc ggactccagt
     5221 tgcgcaacct gttctgtgtt taaacctgct ttgagtaata gtgattgcat cgaaacttgt
     5281 aattcgcgtt gaattgtcag gacgcgatcc aacgtttcct ggctgataac gccttcggtg
     5341 accaaaaact tgccgagcgg cagagaactg cgttcatggc gcaataacaa cacgttaatt
     5401 gctgaacgat taatatgacc gagcgtggtc agtatttcgg cgaacaggaa ctgatgcggc
     5461 acatattgcc gccagatttc accggtctgc tgttccgtga gccactgatg ctgaaccgca
     5521 ttgtacaaca ttgcccgcgg atcgtgaccg cgtcggcgtg cataccagtg acgtaacccc
     5581 gtgacaattt gtccccgcag aacaatgacg taacgcactt tgcgtccgac tttacgcgtc
     5641 agggccgcca gcgaaaccgg gtcaatacca tcttcactgc cgacaattaa ctcatcattt
     5701 tccagacgca gcggcagtac tgcataatgc agcgctacgg aggctggcat ttcggcaatc
     5761 agcgaggacg ggatctgcca ggcatcgatg gattcccacg ccacgccgtt ttgctctgcc
     5821 agcgcctgtg ccagctgctc ggcgctaatc agcccctgca tcagcattga accgcccagg
     5881 cgtagacctt cgacgcgatt acgcagtgct gtatcgagtt gttcttcagt gatgacctga
     5941 ttttccagca gaatttggcc taacgggcgc aacgagcggg tatcgccagt cacgctgggg
     6001 aagtcatgcg ttgttttatc ccacgcgacg cgacgtggat cgccgtgttg aagtacctgt
     6061 tttagcgcgc gccagttggc catgaagtta atcaggttgc cccagaaaag acgcaggaca
     6121 gatagcagcc cctgcgtcag accgtagtag ccagtgacga aaatcacccg ctgcacgatg
     6181 cggttaacca tcaagccaaa gtttagccac agcagggtca ttaaccatgc gctgccgcta
     6241 aaaatagaaa ggaaatgcca ggcattgggc cacaaacttt catacgccag caacagcaaa
     6301 agctggagca tcaccagcat cgcgaggaag ctgacaaagt tactgattgc ccctttgcgg
     6361 tcgcgccaga gaaagtagtt cagcgtcagg ctggaggtcc atttgtgggt tttaaagccc
     6421 tggaaaacaa tgccgatgat ccagcgagat ttttgtcgaa ctgcagtcga aaaggtatcg
     6481 gggaaatatt cgcgcacgca gatcatgttt gacgtccgtg cgtgctgtaa aaatttgcgc
     6541 tgctcgcgtt ctttggcttc gtccaccacc ggaaaacgga caaaaatttc cgtcatacct
     6601 ttctctttca ggcggaaacc aatgtcgtaa tcttcagtca gactctgcac gtcgaaagca
     6661 ataccgtcac cgtcagctaa cagagcggtc acggcgcggc ggctgaaaca ggtgccgacg
     6721 cctgcgctgg gcacttgtcc ggcgagggct tcacgcaccg gaacatcttt gccatgcagc
     6781 tctgaaaact catcaatgta agtcatgctg gtgaagtgcg tccattcgcg ttcgaacgga
     6841 tacaccggga tctgaatcag atctttacgc tcgaccagat agttgaacag acgcaattcc
     6901 atcggtgaaa tcacatcttc ggcgtcatgc agaataaaac cagcaaaagc gaaattggcg
     6961 ctacgttcaa actgggtgat ggcgtccagc acgttgttca gacagtcggc tttgctggtg
     7021 ggaccaggac gcgcgcagac taccttatgc acgttcggaa agcgagcgca cacttcgtca
     7081 acatcacgtt gagtatcggg gtcgttgggg taggtgccaa caaagatatg atagttttcg
     7141 tagtcgagcg tggtcgccgc cagctcggcc atattgccga tgacgcccgt ttcattccac
     7201 gccggaacca taatcgctaa cggtttttca tctggtttat acagttcgcg gtaactcatt
     7261 cgcgggtagc ggcgataaac actcaacttg cgtttaatgc ggcgtaccca gtagacgaca
     7321 tcaataaaaa aatcgtccag cccgctgatg aacatgatga ccgctaacgt tatcgcgatt
     7381 acttttaagc cgtagagcca ggttgcaaaa acatcaagaa gccagtccac acaaaaacct
     7441 tacattaacg ctggttatgt ttagggtggc gtatattaag gttttttatg aattgtgaca
     7501 gctttttacc attaataggt atgactattg cggcacgtta tttttacact ggttataaaa
     7561 gttgccgttt gcgtagaatg tagacaaaag cgtggtgtca aggaggggag gttaacgtca
     7621 tagtgaagag gagtggtggc accgatcagg tcatcatgta gtggaagtgc ctggaggagg
     7681 tgagcaggca gggcaagatt acgaaagccc gctccccgca aggactgacg ccagatagtt
     7741 tctgtccatg gctgcttttc gcatcttacg tcttaaccct gccttgaata ccttatcatt
     7801 cgtcaaaata ttaatagcga tgtgccgtat ccctgaaaat aattctgcgg cgtttcctct
     7861 tcttatt
//
LOCUS       DAPHDW010000181         7710 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.184, whole genome shotgun sequence.
ACCESSION   DAPHDW010000181 DAPHDW010000000
VERSION     DAPHDW010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7710
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.184"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(96..359)
                     /gene="cedA"
                     /locus_tag="RH057_004051"
     CDS             complement(96..359)
                     /gene="cedA"
                     /locus_tag="RH057_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HDV3130340.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            542..2803
                     /gene="katE"
                     /locus_tag="RH057_004052"
     CDS             542..2803
                     /gene="katE"
                     /locus_tag="RH057_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HDV3130341.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGSRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGIHR
                     MGIDTNPANYEPNSINDNWPRETLPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKALK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSADGSFMDELLTLMAAHR
                     VWSRIPKIDKIPA"
     gene            complement(2850..3608)
                     /gene="chbG"
                     /locus_tag="RH057_004053"
     CDS             complement(2850..3608)
                     /gene="chbG"
                     /locus_tag="RH057_004053"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HDV3130342.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDRSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLNV"
     gene            complement(3621..4973)
                     /gene="celF"
                     /locus_tag="RH057_004054"
                     /gene_synonym="chbF"
     CDS             complement(3621..4973)
                     /gene="celF"
                     /locus_tag="RH057_004054"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HDV3130343.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEDG
                     KEKLDIIFELCQRMIDNAGVPMKLYKTLDRREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIRDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKASSVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            complement(5078..5920)
                     /gene="chbR"
                     /locus_tag="RH057_004055"
     CDS             complement(5078..5920)
                     /gene="chbR"
                     /locus_tag="RH057_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HDV3130344.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLERGDFVFIPLGSHHQSFYEFGATRILNVGISKRFFE
                     QHYLPLLPYCFVASQVYRTNNAFLTYVETVISSLNFRETGLEEFVEMVTFYVINRLRH
                     YREEQVIDDVPQWLKSTVEKMHDKEQFSESALENMVTLSAKSQEYLTRATQRYYGKTP
                     MQIINEIRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTPKSYRKKLT
                     EFNQ"
     gene            complement(5928..6278)
                     /gene="chbA"
                     /locus_tag="RH057_004056"
     CDS             complement(5928..6278)
                     /gene="chbA"
                     /locus_tag="RH057_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HDV3130345.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(6329..7687)
                     /gene="chbC"
                     /locus_tag="RH057_004057"
     CDS             complement(6329..7687)
                     /gene="chbC"
                     /locus_tag="RH057_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
                     /protein_id="HDV3130346.1"
                     /translation="MSNVIASLEKVLLPFAVKIGKQPHVNAIKNGFIRLMPLTLAGAM
                     FVLINNVFLSFGEGSFFYSLGIRLDASTIETLNGLKGIGGNVYNGTLGIMSLMAPFFI
                     GMALAEERKVDALAAGLLSVAAFMTVTPYSVGEAYAVGANWLGGANIISGIIIGLVVA
                     EMFTFIVRRNWVIKLPDSVPASVSRSFSALIPGFIILSVMGIIAWALNTWGTNFHQII
                     MDTISTPLASLGSVVGWAYVIFVPLLWFFGIHGALALTALDNGIMTPWALENIATYQQ
                     YGSVEAALAAGKTFHIWAKPMLDSFIFLGGSGATLGLILAIFIASRRADYRQVAKLAL
                     PSGIFQINEPILFGLPIIMNPVMFIPFVLVQPILAAITLAAYYMGIIPPVTNIAPWTM
                     PTGLGAFFNTNGSVAALLVALFNLGIATLIYLPFVVVANKAQNAIDKEESEEDIANAL
                     KF"
BASE COUNT         2064 a         2024 c         1721 g         1901 t
ORIGIN      
        1 cagctttttg ttactggtgg tttgaatgaa tgtagcaatg aaatacagaa tttcaggtca
       61 tgtaactccc ggcaaaaccg ggaggtacgt aatctttact cagtcacttc cccttcctgg
      121 cggatctgat ttgcccaacg ctgggcggat tcaggcacag taaacgccgg tgagcgcaga
      181 aatgactctc ccatcagtac gaacgcaaca tatttgccac gcagcatcca gacatcacga
      241 aacgaatcca tctttatcgc atgttctggc ggcgcgggtt ccgtgcgtgg gacatagcta
      301 ataatctggc ggttttgctg gcggagcggt ttcttcatta ctggcttcac taaacgcata
      361 ttaaaaatca gaaaaactgt agtttagccg atttagcccc tgtacgtccc actttgcgtg
      421 tatttcataa caccgtttcc agaatagtct ccgaagcggg atctggctga tggtctatag
      481 ttagagggtt ttttgaccaa aacagcggcc ctttcagtaa taaattaagg agacgagttc
      541 aatgtcgcaa cataacgaaa agaacccaca tcagcaccag tcaccactac acgattccag
      601 cgaagcgaaa ccggggatgg actcactggc acctgaggac ggatctcatc gtccagcggc
      661 tgaaccaaca ccgccaggtg cacaacctac cgccccaggg agcctgaaag cccctgatac
      721 gcgtaacgaa aaacttaatt ctctggaaga cgtacgcaaa ggcagtgaaa attatgcgct
      781 gaccactaat cagggcgtgc gcatcgccga tgatcaaaac tcactgcgtg ccggtagccg
      841 tggtccaacg ctgctggaag attttattct gcgcgagaaa atcacccact ttgaccatga
      901 gcgcatcccg gaacgtattg ttcatgcacg cggatcagcc gctcacggtt atttccagcc
      961 atataaaagc ttaagcgata tcaccaaagc ggatttcctc tcagatccga acaaaatcac
     1021 cccagtattt gtacgtttct ctaccgttca gggtggtgct ggctctgccg ataccgtgcg
     1081 tgatatccgt ggttttgcca ccaagttcta tactgaagag ggtatttttg acctcgttgg
     1141 caataacacg ccaatcttct ttatccagga tgcgcataaa tttcccgatt ttgttcatgc
     1201 ggtaaaacca gaaccgcact gggcaattcc acaagggcaa agcgcccacg atactttctg
     1261 ggattatgtt tctctgcaac ctgaaactct gcacaacgtg atgtgggcga tgtcggatcg
     1321 cggcatcccc cgcagttacc gcaccatgga aggcttcggt attcacacct tccgcctgat
     1381 taatgccgaa gggaaggcaa cgtttgtacg tttccactgg aaaccactgg caggtaaagc
     1441 ctcactcgtt tgggatgaag cacaaaaact caccggacgt gacccggact tccaccgccg
     1501 cgagttgtgg gaagccattg aagcaggcga ttttccggaa tacgaactgg gcttccagtt
     1561 gattcctgaa gaagatgaat tcaagttcga cttcgatctt ctcgatccaa ccaaactgat
     1621 cccggaagaa ctggtgcccg ttcagcgtgt cggcaaaatg gtgctcaatc gcaatccgga
     1681 taacttcttt gccgaaaacg aacaagcagc attccatcca ggtcatattg ttcctggtct
     1741 ggacttcacc aacgatccgc tgttgcaggg acgtttgttc tcctataccg atacacaaat
     1801 cagtcgtctt ggtgggccga atttccatga gattccgatt aaccgtccga cctgccctta
     1861 ccacaacttc cagcgtgatg gcatacatcg tatggggatc gacactaacc cggcgaatta
     1921 cgaaccgaac tcgatcaacg ataactggcc gcgcgaaaca ctgccgggac cgaaacgcgg
     1981 cggttttgaa tcatatcagg aacgcgtgga aggaaataaa gttcgtgagc gcagtccatc
     2041 gtttggcgaa tattattccc atccgcgtct gttctggcta agtcagacgc cattcgagca
     2101 gcgccatatt gtcgatggtt tcagttttga gttaagcaaa gtcgttcgtc cgtatattcg
     2161 tgagcgcgtt gttgaccagc tggcgcatat tgatctcact ctggcccagg cggtggcgaa
     2221 aaatctcggt atcgaactga ctgacgacca gctgaatatc accccacctc cggacgtcaa
     2281 cggtctgaaa aaggatccat ccttaagttt gtacgccatt cctgacggtg atgtgaaagg
     2341 tcgcgtggta gcgattttac ttaatgatga agtgagatcg gcagaccttc tggccattct
     2401 caaggccctg aaggccaaag gcgttcatgc caaactgctc tactcccgaa tgggtgaagt
     2461 gactgcggat gacggtacgg tgttgcctat agccgctacc tttgccggtg caccttcgct
     2521 gacggtcgat gcggtcattg tcccttgcgg caatatcgcg gatatcgctg acaacggcga
     2581 tgccaactac tacctgatgg aagcctacaa acaccttaaa ccgattgcgc tggcgggaga
     2641 cgcgcgcaag tttaaagcaa caatcaaggt cgctgaccag ggtgaagaag ggattgtgga
     2701 agctgacagc gccgacggta gttttatgga tgaactgtta acgctgatgg cagcacaccg
     2761 cgtgtggtca cgcattccta agattgacaa aattccggcg taaatatgga taaggtcacg
     2821 tcccctgggg agtgacctta ccaccttact tacacattaa gataactacc caaccgataa
     2881 ccacgctcag caattgcata tttcaacgac gctgatgtca gcacttccag ctccgttaac
     2941 cgtgggaaac agtaagcact ctgacggata gtattatcga taaacgccgg atgacacatc
     3001 acctccagcg aacgatcacc ccgatggctt gcgtcatcca gcacttgcag gaacagtgcc
     3061 tcgctaatct cttcaccata aaacgcgcta ctgaatccct gagaactgcg cagattagcc
     3121 ggtaaatcac cagcgttaga tagcggctga cggtcgatgc gcaacgcaat cccctcttca
     3181 gccgcaaacc tggcaacaat cgggaaaatc tgcgggaaca tatgcacatg atgatggcta
     3241 tcaagatgcg taggtttgcg tccaaatagc tcaatgaaac gcaaatactg actggcaagc
     3301 tcctgagtaa tttcttccag cggtaaagca tcttcttctg ccaactgcca gatccatttt
     3361 cccagcacac catcgcgggt taaccccggc atagctgtca gtggcttgcc catagtaagg
     3421 acaaagtgca tccctatggc cagactcggt tcatcacgac tcaactgcac cgcatggtca
     3481 atagcctgcc cattcaccag cgccgtcgtt gatgtgacaa tcccattgcg acaggcctcg
     3541 ataatgccgt agttctgacc tttgcttaag ccaaaatcat cggcattaac aatcagtaag
     3601 cgttccataa tcagcctcgg ttaatgtgct tttttaagct ctgcgatgca gtcggcaaaa
     3661 tttggcagcc atttctcgtg cgccagaatc atctcgcgtg ccagcagctc agcatcgcga
     3721 tcggaatgca ccaacggact aaggtttagc gccagtaaca catcgttaaa ttctccgcta
     3781 agtgcggcgt tgctggcagc aatctcgaag cctttaatgg tgtgaatcag ccccatcact
     3841 ttatcatcga aatgcgtaat gcgcggatgt ggcgtcgcgc catcgcgccc cagcttacag
     3901 gtcatttcta ccgcccagtc tgccggaata ttatcaatat gcccatgatg cgggatatta
     3961 acgtaatgtt cagcttgctt gtcgttgtag atagcgttga tcacttcgca cgctgcatca
     4021 gagtaataag ccccaccgcg ctgttccagt tctttcggct taactttcaa ctccggattt
     4081 ttatacagct caaaaagttg tttctcgact ttctgtacta cctgtgctcg tgcgccgcct
     4141 ttgtagtatt cgcccatttc aatagccagc atctctttct gcttgaagta atacagcaga
     4201 taagaacatg gcagcagatt taacgaacga attaagccct cactaaatgg cagatcgaaa
     4261 atatttttta cagaggacgc ttttaactgc cctgacgcca caccatcaag caattcggca
     4321 aagcgcgact tgccatttac cagcacatcc ttaatgaaca ccatatggtt gaggccgaac
     4381 agatcgatag ataaatcatc gctgtctttc agcatcagaa catcgcgaat aaacatcttc
     4441 atgccgatcg gaatattaca cacgccgata aagcgtttaa atccggtatg acgataaacg
     4501 gcttcagtga ccattcccgc cgggttagtg aagttaatca cccatgcatt cggacaaagt
     4561 tcttcgacat cttttacgat gtcaaaaatc accggaatgg tacgcagacc tttaaacaga
     4621 ccgcccgcgc cgttggtttc ctgaccaaga taaccatgac ttaatggaat acgttcatcc
     4681 agttcgcgcg ccggtaattg gcctacgcgc agttgggtag taacgaaatc agcatctttc
     4741 aatgcttcgc ggcgatccag cgttttataa agcttcatcg ggacgccagc gttatcaatc
     4801 atccgttggc agagttcaaa aatgatatcc agtttctctt taccatcttc gacatccacc
     4861 agccataatt cgctgaccgg caattcgtga taacgcttaa taaatccttc cagtaactcc
     4921 ggggtatagc tgctcccgcc accaatagtg acgactttta atttctggct cataattttt
     4981 cccttcagta cagaatactg atatctggca tatctgcccc cccggacata aataatccag
     5041 caacaggaca gatatgtgaa ttgtcaggta taacgactta ctgattaaat tcagtcaatt
     5101 tcttacgata gctcttaggc gtaaaggacg ttaatttctt aaacgtttta ataaacaaac
     5161 tcgggctact ataaccggcc tcaaacgcaa tatccgtcac ggaatagttg gtcatttcca
     5221 gttgtttttt ggcaaaatta atacggatct cattaataat ctgcattggt gttttgccat
     5281 aatatcgttg agtcgctcgc gtcaaatatt cctgtgattt ggctgacaac gtcaccatat
     5341 tctccagcgc cgattcacta aactgctctt tatcatgcat cttttctacc gtacttttca
     5401 gccactgcgg tacatcatca atcacctgtt cttcgcggta atgacgtaaa cggttaatga
     5461 cataaaaagt aaccatctca acaaactctt ccagccctgt ttcgcggaaa ttcaatgaag
     5521 aaatcactgt ttccacatag gtgagaaaag cgttattggt ccggtatacc tgcgaagcga
     5581 caaagcaata aggcaacaac ggcaggtaat gctgctcaaa aaagcgttta ctgatcccaa
     5641 cgttcaatat acgcgtggca ccaaactcat aaaaactttg atggtgcgaa cctaacggaa
     5701 taaaaacaaa atcgccccgt tccagtaaca cgcgcttacc gttaatctcc tggaaataac
     5761 gcccggttaa taccagagta aattcataat agtcgtgctg atgcagcccg ctgatactct
     5821 cagttttgtt atagataaac acatggaagt ttttgccatt aaacaactgc tgttctcggg
     5881 cagtggcaat ttccggcgcg ttaatcactg gctgcatcat cgactcctta tgccttcagt
     5941 ttttcatgaa gctcaattaa ttcagtaatc agttcacgcg caagcatgga cgtcattaaa
     6001 tgatcctgag cgtggaccag caccagactc actttcatct taccttcgcc cgcatcgcct
     6061 tcaatcagtt tcgtctgtac cagatgcgct tcattcaatg ccatccgtga ctgatccatc
     6121 atggcttttg ctgcagcaaa atcgccctgc ttcgcctgtt tcagtgccgc atacgccagg
     6181 ctgcgcgctt gtccggagtt gatgataagc cccatcacca cttcttccag ctcttcagct
     6241 tccgtttgcg tatcgggaat gttatcgaga tccatcatac atcgttcctc tctattctta
     6301 ccggcacgat tacccgtacc ggcatcgatt aaaatttcag ggcgttagcg atatcttctt
     6361 cgctctcttc tttatcaatc gcgttttgtg ctttgttagc caccacaaca aagggcagat
     6421 aaattaacgt tgcgatacca aggttgaaga gtgcgaccag caatgcggcg acgctaccgt
     6481 tggtgttaaa gaaggctccc agaccggttg gcatggtcca cggtgcaata ttggtcaccg
     6541 gaggaatgat gcccatgtag tacgctgcga gggtgattgc cgccagaatc ggttgtacca
     6601 gtacaaacgg gataaacatc accgggttca tgataattgg cagaccaaac agaatcggtt
     6661 cgttaatctg gaagatgccg gacggcagcg ccagttttgc cacctgacga taatcagcac
     6721 ggcgagaggc gataaagata gccaggatca ggcctaaagt cgcaccactg cccccaagga
     6781 aaataaagga gtccagcatc ggcttggccc agatatggaa ggtcttaccg gctgccagcg
     6841 ccgcttcgac ggaaccatat tgctgatagg tcgcgatatt ttccagcgcc cacggcgtca
     6901 taatgccgtt gtccagtgcg gtcagcgcca gcgcgccatg aataccgaag aaccagagca
     6961 gtggaacaaa gatcacatag gcccagccca ccacgctacc caacgatgcc agtggggttg
     7021 agatggtatc cataatgatc tgatggaagt tggtgcccca ggtattcaac gcccaggcaa
     7081 taatccccat cacggaaaga ataataaagc cgggaattaa tgccgagaag gaacgcgata
     7141 ctgaagcagg tacgctgtcg ggcagtttaa tgacccaatt gcggcggaca ataaaggtaa
     7201 acatttctgc caccaccagg ccaataataa tcccggagat gatattcgcc ccacctaacc
     7261 agtttgcgcc aaccgcatag gcctcaccga cactatatgg ggtgacggtc ataaatgctg
     7321 caacggataa caacccagcc gccagcgcat cgactttacg ctcttctgcc agcgccatgc
     7381 caataaagaa cggtgccatt aaagacatta tccctaatgt tccgttatat acgttgccgc
     7441 caataccttt cagaccatta agtgtttcaa tggttgaggc gtcgaggcga atacctaagg
     7501 aataaaaaaa cgacccctcc ccaaagctta gaaaaacgtt gttaattaat acaaacatgg
     7561 cccccgcaag ggttaacggc attaagcgaa taaagccatt tttgattgca ttaacgtgtg
     7621 gctgctttcc tattttaact gcaaaaggga ggagtacctt ttcaagcgat gcaataacat
     7681 tactcataga aaaataccct taaaaaccgc
//
LOCUS       DAPHDW010000182         7681 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.185, whole genome shotgun sequence.
ACCESSION   DAPHDW010000182 DAPHDW010000000
VERSION     DAPHDW010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7681
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.185"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..222
                     /gene="yafQ"
                     /locus_tag="RH057_004058"
     CDS             <1..222
                     /gene="yafQ"
                     /locus_tag="RH057_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafQ"
                     /protein_id="HDV3130347.1"
                     /translation="QKRHKDMNKLKYLMTLLINNTLPLPAVYKDHPLQGSWKGYRDAH
                     VEPDWILIYKLTDKLLRFERTGTHAALFG"
     gene            378..1118
                     /locus_tag="RH057_004059"
     CDS             378..1118
                     /locus_tag="RH057_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HDV3130348.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVVN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            complement(1089..1856)
                     /locus_tag="RH057_004060"
     CDS             complement(1089..1856)
                     /locus_tag="RH057_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HDV3130349.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRQKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(2062..2640)
                     /gene="lpcA"
                     /locus_tag="RH057_004061"
     CDS             complement(2062..2640)
                     /gene="lpcA"
                     /locus_tag="RH057_004061"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HDV3130350.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            2880..5324
                     /gene="fadE"
                     /locus_tag="RH057_004062"
     CDS             2880..5324
                     /gene="fadE"
                     /locus_tag="RH057_004062"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HDV3130351.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLAIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLAGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYAHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMAVLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDDLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKVAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALAKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            complement(5367..5813)
                     /gene="ivy"
                     /locus_tag="RH057_004063"
     CDS             complement(5367..5813)
                     /gene="ivy"
                     /locus_tag="RH057_004063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HDV3130352.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     TIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            5994..6764
                     /gene="yafV"
                     /locus_tag="RH057_004064"
     CDS             5994..6764
                     /gene="yafV"
                     /locus_tag="RH057_004064"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HDV3130353.1"
                     /translation="MPGLKITLLQQPLVWMDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYYLAI
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHQATRIDAELSMVALREYREKFPAWQDADEFRLR"
     gene            complement(6806..>7681)
                     /locus_tag="RH057_004065"
     CDS             complement(6806..>7681)
                     /locus_tag="RH057_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV3130354.1"
                     /translation="ECFINWMRDCHSSDDKDVIAIDGKTLRHSYDKSRRRGAIHVISA
                     FSTMHSLVIGQIRTDEKSNEITAIPELLNMLDIKGKIITTDAMGCQKDIAEKIQKQGG
                     DYLFAVKGNQGRLNKAFEEKFPLKELNNPEHDSYAMSEKSHGREEIRLHIVCDVPDEL
                     IDFTFEWKGLKKLCVAVSFRSIIAEQKKEPEMTVRYYISSADLTAEKFATAIRNHWHV
                     ENKLHWRLDVVMNEDDCKIRRGNAAELFSGIRHIAINILTNDKVFKAGLRRKMRKAAM
                     DRNYLASVLAESGLS"
BASE COUNT         1786 a         1944 c         2010 g         1941 t
ORIGIN      
        1 caaaagcgtc ataaggatat gaataaattg aaatatctta tgacgcttct tatcaataat
       61 actttaccgc ttccagctgt ttataaagac cacccgctgc aaggttcatg gaaaggttat
      121 cgcgatgctc atgtcgaacc ggactggatc ctgatttaca aacttaccga taaactttta
      181 cgatttgaga gaactggaac tcacgcggcg ctctttgggt aaagcacgct gccgatgaaa
      241 tttgtaaacc ctgcgccgac aggcttgcgt aaaactcttt tggtgataat gtgagccttc
      301 caaatccaga ctattccgat ttgggatcta ttttgccgtc ctggcgtgtg taaccgtttt
      361 atcaaggaat aagcagtatg cgtaaaatcg cattaattct tgcgatgttg ttgatcccgt
      421 gcgtttcgtt tgccggtttg ctgggcagca gtagctcgac cacgccagtg agcaaagagt
      481 ataagcagca gttgatggga tcccctgtct acatccagat cttcaaggaa gaacgtacgc
      541 tcgatctcta cgtcaaaatg ggcgagcaat atcagctgct cgacagttat aaaatctgta
      601 aatattccgg cggcttaggg ccaaaacagc gtcagggcga tttcaaaagc ccggaagggt
      661 tttatagcgt ccagcgtaat cagttaaaac cagacagccg ttactacaaa gcgattaata
      721 ttggtttccc caatgcctat gaccgtgcgc atggttatga agggaaatac ctgatgatcc
      781 acggcgattg tgtttccatc ggctgctacg caatgaccaa tcagggtatt gatgagatct
      841 tccagttcgt tactggtgcg ctggtgtttg gtcagccgag cgtgcaagtg agtatttacc
      901 cgttccgcat gaccgacgcc aatatgaagc gccataaata ttccaacttt aaggacttct
      961 gggagcaact gaagccgggt tacgactact ttgagcaaac ccgtaagcca ccgaccgttt
     1021 ctgtggtcaa tggtcgttac gtggtcagca agccgttaag ccacgaagtt gtgcagccac
     1081 aactggcatc aaactacacg ctccccgagg caaaataagc gccattcgcc tggcataatc
     1141 ttttgccagg tttcattgcc cgtcagcggc tgtgttgcaa tcaccgtgac cacatcattc
     1201 ggtgtggtct gcgagctgaa gtcgatttcc acatcctgat ccagcagcgt tgccacgcca
     1261 aacggcgcgc ggcgggtgat ccagtgtaaa ttagtcgagc aatacgccat cacatagcgc
     1321 ccgtcagaaa gcagcatgtt gaaaacgccc ttctgccgca gttcatccgc cagtgaggcg
     1381 atatatttaa acactgccgc catgttgccc ggtgtgcgcg ggtaacgctg cgttaattta
     1441 tgcaggagcc agcagaaggc tttttcgcta tcggtttcac cgaccgggcg gaagttgccg
     1501 gtttccagtg atttgtagcc cgtcagttgt ccgttatggg cataagtcca gttacgcccc
     1561 cataactcgc gggtaaatgg gtgagtattt tccagcgcca cctcgccccg attagcctgg
     1621 cgaatatgag ccaccaccga acaggatttt atcgggtagt cctggacaag tttggcgatg
     1681 ggggaattaa agctgggttg tggatcttta aatgtgcgac agcctttacc ttcgtaaaag
     1741 gtaatgcccc agccatcttt atgtggcccg gttccaccac cacgctgtac aagtccggtg
     1801 aaactaaagc agatatcggt agggacgttg gcgctcatcc cgagcaattc gcacatgggt
     1861 aacctccaca acagcgggag cgcatggccc ccggcgaaga ttgatttaag aattttcagg
     1921 tcggatgcgg cgtaaacgaa ttattcggcc tacggttcgg gtacagattt gtaggcatga
     1981 taagacgcgt cagcatcgca tcaggcatca gcgcacaagt gccggatgcg gcgtaaacgc
     2041 cttatccggc ctacgccaga cttacttaac catctctttt tcaattaact ggatcaggat
     2101 atggatcact ttaatgtgaa tctcctgaat gcggtcggca taaccaaagt gcggtacgcg
     2161 aatttcgata tccgccgtgc cagccatttt gccgccgtct ttaccggtca gggtgatcac
     2221 tttcatcccc ttctcacgcg ccgctgcgat cgctttgatc acgtttgcag agttaccgga
     2281 ggtggagatc cccagcagta catcgccttc gcgacctacc gcttcaacgt agcgggagaa
     2341 aatatcattg aaaccgaaat cattaccgac gcaggaaata tgactaacgt cagaaatagc
     2401 aatcgccggg tagcccggac ggttttcacg gtagcgaccg gtcaactctt cggcaaagtg
     2461 catagcgtcg caatgggaac cgccgttgcc gcaggaaagc actttgccac cggctttaaa
     2521 gctgtctgct aacaggaccg ccgcgcgctg aatggcgtga atattggcgt catcttttaa
     2581 aaagttagcc agcgtttccg ccgcttcgtt cagttcgtta cgaataagat cctggtacat
     2641 gaggatatcc ttcagcataa atgtaataga caaaatgcag tgtaccggat accgccaaaa
     2701 gcgagaagta cgggcaggtg ctatgaccag gactttttga cctgaagtgc ggataaaaac
     2761 agcaacaatg tgagctttgt tgtaattata ttgtaaacac attgctaaat gtttttacat
     2821 ccactacaac catatcatca caagtggtca gacctcctac aagtaagggg cttttcgtta
     2881 tgatgatttt gagtattctc gctacggttg tcctgctcgg cgcgttgttc tatcaccgcg
     2941 tgagcttatt tatcagcagt ctgattttgc tcgcctggac agccgccctc ggcgttgctg
     3001 gtctgtggtc ggcgtgggta ctggtgcctc tggccattat cctcgtgcca tttaactttg
     3061 cgcctatgcg taagtcgatg atttccgcgc cggtatttcg cggtttccgt aaggtgatgc
     3121 cgccgatgtc gcgcactgag aaagaagcga ttgatgcggg caccacctgg tgggagggcg
     3181 acttgttcca gggcaagccg gactggaaaa agctgcataa ctatccgcag ccgcgcctga
     3241 ccgccgaaga gcaagcgttt ctcgccggcc cggtagaaga agcctgccgg atggcgaatg
     3301 atttccagat tactcatgag ctggcggatc tgccgccgga gttgtgggcg taccttaaag
     3361 agcatcgctt cttcgcgatg attatcaaaa aagagtacgg cggactggag ttctcagctt
     3421 atgcccagtc tcgcgtgctg caaaaactct ccggcgtgag cgggatcctg gctattaccg
     3481 tcggcgtgcc gaactcatta ggcccgggcg agctgctgca acattacggc actgacgagc
     3541 agaaagatca ctatctgccg cgtctggcgc gtggtcagga gatcccttgc tttgcactga
     3601 caagcccgga agcgggttcc gatgcgggcg cgattccgga caccgggatt gtctgcatgg
     3661 gcgaatggca gggccagcag gtgctgggga tgcgtctgac ctggaacaaa cgctatatta
     3721 cgctggcacc gattgcgact gtgcttgggc tggcgtttaa actctccgac ccggaaaagc
     3781 tgctgggtgg cgcagaagat ttaggtatta cctgtgcgct gatcccgacc accacgccgg
     3841 gcgtggaaat tggtcgtcgc cacttcccgc tgaacgtacc gttccagaac ggaccgacgc
     3901 gcggtaaaga tgtcttcgta ccgatcgatt acatcatcgg ggggccgaaa atggccgggc
     3961 aaggctggcg gatgctggtg gagtgcctct cggtaggccg cggcatcacc ctgccttcca
     4021 actcaaccgg cggcgtgaaa tcggtagcgc tggcaacggg cgcgtatgct cacattcgcc
     4081 gtcagttcaa aatctctatc ggtaagatgg aagggattga agagccgctg gcgcgtattg
     4141 ccggtaatgc ctacgtgatg gatgctgcgg catcgctgat tacctacggc attatgctcg
     4201 gcgaaaaacc tgccgtgctg tcggctatcg ttaagtatca ctgtacccac cgcgggcagc
     4261 agtcgattat tgatgcgatg gatatcactg gtggcaaagg cattatgctc gggcaaagca
     4321 acttcctggc gcgtgcttac cagggcgcac cgattgccat caccgttgaa ggggctaaca
     4381 ttctgacccg cagcatgatg atcttcggac aaggagcgat tcgttgccat ccgtacgtgc
     4441 tggaagagat ggaagcggcg aagaacaatg acgtcaacgc gttcgataaa ctgttgttca
     4501 aacatatcgg tcacgtcggt agcaacaaag ttcgcagctt ctggctgggc ctgacgcgcg
     4561 gtttaaccag cagcacgcca accggcgatg ccactaaacg ctactatcag cacctgaacc
     4621 gcctgagcgc caacctcgcc ctgctttctg atgtctcgat ggcagtgctg ggcggcagcc
     4681 tgaaacgtcg cgagcgcatc tcggcccgtc tgggggatat tttaagccag ctctacctcg
     4741 cctctgccgt gctgaagcgt tatgacgacg aaggccgtaa tgaagccgac ctgccgctgg
     4801 tgcactgggg cgtacaagat gcgctgtatc aggctgaaca ggcgatggat gatttactgc
     4861 aaaacttccc gaaccgcgtg gttgccgggc tgctgaatgt ggtgatcttc ccgaccggac
     4921 gtcattatct ggcaccttct gacaagctgg atcataaagt ggcgaagatt ttacaagtgc
     4981 cgaacgccac ccgttcccgc attggtcgcg gtcagtacct gacgccgagc gagcataatc
     5041 cggttggctt gctggaagag gcgctggtgg atgtgattgc cgccgaccca attcatcagc
     5101 ggatctgtaa agagctgggt aaaaacctgc cgtttacccg tctggatgaa ctggcgcaca
     5161 acgcgctggc gaaggggctg attgataaag atgaagccgc tattctggtg aaagctgaag
     5221 aaagccgtct gcgcagtatt aacgttgatg actttgatcc ggaagagctg gcgacgaagc
     5281 cggtaaagtt gccggagaaa gtgcggaaag ttgaagccgc gtaaatgaat aacggagccg
     5341 aaaggctccg tttctttatc cgctaattat ttaaaattaa agccatccgg atggttttcc
     5401 aggctgccgg tcaacgccgc gaacaacacc gttttaccat caatcgaaag cgcatcgttc
     5461 acattcagcc aggtgagttt ctcttgcgac gttttctcat caatagtcga gaacagcccc
     5521 gtcatctgat tagatttctc ggaccacatc acagcgatac gttgcgagcc acagtcatgc
     5581 ggtttgcacg cgctcatcac ctgatacgtc tcatctccca acgttacggt ttgtgcggga
     5641 gtataagtac cgcctttcat cacccaggca ggcagcttat gcccttgtac catctgatta
     5701 aatgcagctt tggtggtttc gccctttgca aggctgctaa tggttaaatc atcctgcgcc
     5761 attgcactgg tggcgatgac cagagcggcg actgtcgtta ttgccttaaa catcattcct
     5821 cccgagctta tcctgctcat gttattaacc ttctaaagcc tggcgataac acgataattt
     5881 gcaaatcaga tatttccaat atcagcattt gttatcaatt tattctgcgc taaggcaact
     5941 gcctgataag gtgttaaagt tataaaaccc ttcgcataaa ggagttgtac accgtgcctg
     6001 gtttgaagat tacgcttttg cagcaaccac tggtgtggat ggatggccct gccaacctgc
     6061 gtcattttga tcgtcaactg gaaggtatta ccgggcgcga tgtgatcgtt ctaccggaga
     6121 tgtttaccag cggctttgcc atggaagcgg cagcttcgtc gctagcacaa gatgacgtag
     6181 tgaactggat gacagctaag gcgcagcagt gcaatgcgct gattgcaggc agtgttgcat
     6241 tacaaacgga gtctggttcg gttaaccgct ttttgctggt tgagccgggc ggcacggtac
     6301 atttttatga taagcgtcat ctgttccgca tggcagatga gcatctacat tataaagcgg
     6361 gcaatgcgcg agtgattgtg gaatggcgcg gctggcgtat tttgccgttg gtgtgctacg
     6421 acttacgttt tccggtgtgg tcgcgcaatc tcaacgatta ttacctcgcc atctacgttg
     6481 ccaactggcc tgctccgcgc tctctgcact ggcaggcatt gctgacggcc cgcgcgattg
     6541 agaatcaggc gtatgtggcg ggatgcaatc gcgtcggcag cgatggcaac ggctgccatt
     6601 atcgcggtga cagccgggtg attaacccgc agggcgagat tatcgctact gccgacgcgc
     6661 atcaggcaac gcgcattgat gcggagctgt cgatggtggc attgcgggaa tatagagaaa
     6721 agtttccggc atggcaggat gcggatgagt ttaggttgcg gtgagtgggg gttgtgttta
     6781 aggctggggg agagtcgggg taagactacg aaagcccgct ctccgcaagg actgacgcca
     6841 gatagtttct gtccatagct gcttttcgca tcttacgtct taaccctgcc ttgaatacct
     6901 tatcattcgt caaaatatta atagcgatgt gccgtatccc tgaaaataat tctgcggcgt
     6961 ttcctcttct tattttgcag tcgtcttcat tcattaccac gtccagacgc cagtgcagct
     7021 tattctccac gtgccagtgg tttcggattg ctgtggcgaa cttttctgcg gttaaatcag
     7081 cagaactgat ataatatctg accgtcattt ctggctcttt cttttgttct gctattattg
     7141 accgaaagga gactgccacg cataatttct tcagtccttt ccattcaaac gtgaaatcaa
     7201 taagttcatc agggacatcg caaacaatat gaagacggat ttcttctctg ccgtgactct
     7261 tttcactcat tgcgtaactg tcatgctctg gattatttaa ttctttcagc ggaaattttt
     7321 cctcaaaggc tttatttagc cgcccctggt ttccttttac cgcgaataaa taatcacctc
     7381 cctgtttttg tatcttctct gcaatatctt tctggcaacc catcgcatca gttgtgatga
     7441 tttttccttt aatatccagc atgttaagaa gttctgggat agctgtaatc tcattagatt
     7501 tctcatccgt cctgatctgt ccgatgacca gactgtgcat tgttgagaac gcactaatga
     7561 catgaatcgc tcccctgcgg cgactcttgt cataagagtg ccggagcgtt tttccatcaa
     7621 ttgcgatgac gtctttatca tctgaagaat ggcagtcacg catccagtta ataaagcact
     7681 c
//
LOCUS       DAPHDW010000183         7640 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.186, whole genome shotgun sequence.
ACCESSION   DAPHDW010000183 DAPHDW010000000
VERSION     DAPHDW010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.186"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            312..4565
                     /gene="fdeC"
                     /locus_tag="RH057_004066"
     CDS             312..4565
                     /gene="fdeC"
                     /locus_tag="RH057_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000092554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intimin-like adhesin FdeC"
                     /protein_id="HDV3130355.1"
                     /translation="MSRYKTGHKQPRFRYSVLARCVAWANISVQVLFPLAVTFTPVMA
                     ARAQHAVQPRLSMGNTTVTADNNVEKNVASFAANAGTFLSSQPDSDATRNFITGMATA
                     KANQEIQEWLGKYGTARVKLNVDKDFSLKDSSLEMLYPIYDTPTNMLFTQGAIHRTDD
                     RTQSNIGFGWRHFSGNDWMAGVNTFIDHDLSRSHTRIGVGAEYWRDYLKLSANGYIRA
                     SGWKKSPDIEDYQERPANGWDIRAEGYLPAWPQLGASLMYEQYYGDEVGLFGKDKRQK
                     DPHAISAEVTYTPVPLLTLSAGHKQGKSGENDTRFGLEVNYRIGEPLEKQLDTDSIRE
                     RRVLAGSRYDLVERNNNIVLEYRKSEVIRIALPERIEGKGGQTLSLGLVVSKATHGLK
                     NVQWEAPSLLAEGGKITGQGSQWQVTLPAYRPGKDNYYAISAVAYDNKGNTSKRVQTE
                     VVITGAGMSADRTALTLDGQSRIQMLANGNEQRPLVLSLRDAEGQPVTGMKDQIKTEL
                     TFKPAGNIVTRTLKATKSQAKPTLGEFTETEAGVYQSVFTTGTQSGEATITVSVDGMS
                     KTVTAELRATMMDVANSTLSANEPSGDVVADGQQVYTLTLTAVDSEGNPVTGEASRLR
                     FVPQDTNGVTVGAISEIKPGVYSATVSSTRAGNVVVRAFSEQYQLGTLQQTLKFVAGP
                     LDAAHSSITLNPDKPVVGGTVTAIWTAKDANDNPVTGLNPDAPSLSGAAAVGSTASGW
                     TDNGDGTWTAQISLGTTAGELEVMPKLNGQDAAANAAKVTVVADALSSNQSKVSVAED
                     HVKAGESTTVTLVAKDAHGNAISGLSLSASLTGTASEGATVSSWTEKGDGSYVATLTT
                     GGKTGELRVMPLFNGQPAATEAAQLTVIAGEMSSANSTLVADNKAPTVKTTTELTFTV
                     KDAYGNPVTGMKPDAPVFSGAANTGSERPSAGNWTEKGNGVYVSTLTLGSAAGQLSVM
                     PRVNGQNAVAQPLVLNVAGDASKAEIGDMTVKVNNQLANGQSANQITLTVVDSYGNPL
                     QGQEVTLNLPQGVTSKTGNTVTTNAAGKADIELISTVAGELEIAAAVKNSQKTVTVKF
                     NADASTGQANLQVDTAVQKVANGKDAFTLTATVEDKNGNPVPGSLVTFNLPRGVKPLT
                     GDNVWVKANDEGKAELQVVSVTAGTYEITASAGNSQPSNTQTITFVADKATATVSGIE
                     VMGNYALADGKAKQTYKVTVTDANNNLVKDSEVTLTASPASLNLEPNGTATTNEQGQA
                     IFTATTTVAAIYTLKAQVSQTNGQVSTKTAESKFVADDKNAVLTASSDMQSLVADGKS
                     TAKLEVTLMSANNPVGGNMWVDIQTPEGVTEKDYQFLPSKNDHFVSGKITRKFSTSKP
                     GVYTFTFNALTYGGYEMKPVTVTITAVDADTAKDEEAMK"
     gene            complement(4686..5543)
                     /locus_tag="RH057_004067"
     CDS             complement(4686..5543)
                     /locus_tag="RH057_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3130356.1"
                     /translation="MIRQKILQQLLEWIECNLEHPISIEDIAQKSGYSRRNIQLLFRN
                     FMHVPLGEYIRKRRLCRAAILVRLTAKSMLDIALSLHFDSQQSFSREFKKLFGCSPRE
                     YRHRDYWDLANIFPSFLIRQQQKTECRLINFPETPIFGNSFKYDIEVSNKSPDEEVKL
                     RRHHLARCMKNFKTDIYFVSTFEPSTKSVDLLTVETFAGTVCKPTDSEMPKEWTINRG
                     LYASFRYEGEWEHYPEWARNLYLMELPARGLARVNGSDIERFYYNEDFVEKDSNDVVC
                     EIFIPVRPV"
     gene            5792..6661
                     /locus_tag="RH057_004068"
     CDS             5792..6661
                     /locus_tag="RH057_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308365.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HDV3130357.1"
                     /translation="MEFSVLSNNLKMPMMGFGVFQVTDKNVCKQSVLNAIRTGYRLID
                     TAAVYGNEDAVGEAVREAISEGLCTRDELFITSKLWVQDMLNQDTAAAGIEASLKKSG
                     LEYFDLYLLHQAMRDYFSAWRALEDAYEEGKLKAIGVSNFYPHVLANFCETVRVKPMV
                     NQVELHPYFAQPEALATMKYYNVQPEAWAPLGGGRHKPFENNLLQSIADAHQKSISQV
                     ILRWNIQRGVVVIPKSTHQQRIEENFAIWDFSLTEKEMAQISSLDLGYVGESVKHFNP
                     EFVRGCLAVKIHD"
     gene            complement(6821..7414)
                     /gene="rclC"
                     /locus_tag="RH057_004069"
     CDS             complement(6821..7414)
                     /gene="rclC"
                     /locus_tag="RH057_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reactive chlorine species resistance protein
                     RclC"
                     /protein_id="HDV3130358.1"
                     /translation="MEKYLHLLSRGDKIGLTLIRLSIAIVFMWIGLLKFVPYEADSIT
                     PFVANSPLMSFFYEHPEDYKQYLTHEGEYKPEARAWQTANNTYGFSNGLGVVEVIIAL
                     LVLANPVNRWLGLLGGLMAFTTPLVTLSFLITTPEAWVPALGDAHHGFPYLSGAGRLV
                     LKDTLMLAGAVMIMADSAREILKQRSNESSSTLKTEY"
     gene            complement(7426..>7640)
                     /locus_tag="RH057_004070"
     CDS             complement(7426..>7640)
                     /locus_tag="RH057_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HDV3130359.1"
                     /translation="ATLLSGVMAFSANADDKIILKHISVSSVSASPTVLEDTIADIAR
                     KYNASSWKVTSMRIDNNSTATAVLYK"
BASE COUNT         2104 a         1689 c         1946 g         1901 t
ORIGIN      
        1 tagttgttaa ttaaaggcag gccattatta ttcactgtcc gcagagtaaa aaatataact
       61 taggtgtcta tttaatgact tgcacaaaaa gctaaatttt cacccataaa taaaaatata
      121 atcccgcgcc caaccacctg atgagtggct acagacactg gatatattag gtggcggtgc
      181 actttcttac ataaaggtat ttccttttct gcggaaaagg aaatcgggaa atccccggtt
      241 tttctgacaa gcagacgcca ttatttgtgt ctgcctatgt tcgttaattc gttcatcagg
      301 aaattatctc aatgtcgcgt tataaaacag gtcataaaca accacgattt cgttattcag
      361 ttctggcccg ctgcgtggcg tgggcaaata tctctgttca ggttcttttt ccactcgctg
      421 tcacctttac cccagtaatg gcggcacgtg cgcagcatgc ggttcagcca cggttgagca
      481 tgggaaatac tacggtaact gctgataata acgtggagaa aaatgtcgcg tcgtttgccg
      541 caaatgccgg gacattttta agcagtcagc cagatagcga tgcgacacgt aactttatta
      601 ccggaatggc cacagctaaa gctaaccagg aaatacagga gtggctcggg aaatatggta
      661 ctgcgcgcgt caaactgaat gtcgataaag atttctcgct gaaggattct tcgctggaaa
      721 tgctttatcc gatttatgat acgccaacaa atatgttgtt cactcaggga gcaatacatc
      781 gtaccgacga tcgtactcag tcaaatattg gttttggctg gcgtcatttt tcaggaaatg
      841 actggatggc gggggtgaac acctttatcg accatgattt atcccgtagt catacccgca
      901 ttggtgttgg tgcggaatac tggcgcgatt atctgaaact gagcgccaat ggttatattc
      961 gggcttctgg ctggaaaaaa tcgccggata ttgaggatta tcaggaacgc ccggcgaatg
     1021 gttgggatat ccgcgcagag ggctatttac ctgcctggcc gcagcttggc gcaagcctga
     1081 tgtatgaaca gtattatggc gatgaagtcg ggctgtttgg taaagataag cgccagaaag
     1141 acccgcatgc tatttctgcc gaggtgacct atacgccagt gcctcttctg acactgagcg
     1201 ccgggcataa gcagggcaag agcggtgaga atgacactcg ctttggcctg gaagttaact
     1261 accgaattgg cgaacctctg gagaaacaac tcgatacgga tagcattcgc gagcgtcggg
     1321 tactggcagg cagccgctat gacctggttg agcgtaataa caacatcgtt cttgagtacc
     1381 gcaaatctga agtgatccgt attgctctgc ctgagcgtat tgaaggtaag ggcggtcaga
     1441 cactttccct ggggcttgtg gtcagcaaag caactcacgg actgaaaaat gtgcagtggg
     1501 aagcgccgtc attactggct gaaggtggca aaattaccgg tcagggtagt cagtggcaag
     1561 taacgctccc ggcttatcgt ccaggcaaag acaattatta tgcgatttct gcggttgcct
     1621 acgataacaa aggcaatacc tcaaaacgcg tgcagacaga ggtggtcatt accggagctg
     1681 gtatgagcgc cgatcgcacg gcgttaacgc ttgacggtca gagccgtatt caaatgcttg
     1741 ctaacggtaa tgagcaaaga ccgctggtgc tgtctctgcg cgacgccgag ggccagccag
     1801 tcacgggcat gaaagatcag atcaagactg aactaacttt caaaccggct ggaaatattg
     1861 tgactcgtac cctgaaggcc actaaatcac aggcaaagcc aacactgggt gagttcaccg
     1921 aaactgaagc aggggtgtat cagtctgtct ttactactgg aacgcagtca ggtgaggcaa
     1981 cgattactgt tagtgttgat ggcatgagca aaaccgtcac tgcagaactg cgggccacga
     2041 tgatggatgt ggcaaactcc accctgagcg ctaacgagcc gtcaggtgac gtggttgctg
     2101 atggtcagca agtctatacg ttgacgttga ctgcggtgga ctccgagggt aatccggtga
     2161 cgggagaagc cagccgcttg cgatttgttc cgcaagacac taatggtgta accgttggtg
     2221 ccatttcgga aataaaacca ggcgtttaca gcgccacggt ttcttcgacc cgtgccggaa
     2281 acgttgttgt gcgtgctttc agcgagcagt atcagctggg cacattacaa caaacgctga
     2341 agtttgttgc cgggccgctt gatgcagcac attcgtccat cacactgaat cctgataaac
     2401 cggtggttgg cggtacagtt acggcaatct ggacggcaaa agatgctaat gacaaccctg
     2461 taactggtct caatccggat gcaccgtcat tatcgggcgc agctgctgta ggttctacgg
     2521 catcaggctg gacggataat ggcgacggga cctggactgc gcagatttct ctcggcacta
     2581 cggcgggtga attagaagtt atgccgaagc taaatggaca ggatgcggca gcaaatgcgg
     2641 caaaagtaac cgtggtggct gatgcgttat cttcaaacca gtcgaaagtc tctgtcgcag
     2701 aagatcacgt aaaagccggc gaaagtacaa ccgtgacgct ggtggcgaaa gatgcgcatg
     2761 gcaacgctat cagtggtctt tcgttgtcgg caagtttgac ggggaccgcc tctgaagggg
     2821 cgaccgtttc cagttggacc gaaaaaggtg acggttccta tgttgctacg ttaactacag
     2881 gcggaaagac gggcgagctt cgtgtcatgc cgctattcaa cggccagcca gcagccaccg
     2941 aagccgcgca gttgacggtc atcgccggag agatgtcatc agcgaactct acgcttgttg
     3001 cggacaataa ggctccgacc gtcaaaacga cgacggaact caccttcacc gtgaaggatg
     3061 cgtacgggaa cccggtcacc gggatgaagc cagatgcacc agtgtttagc ggtgccgcca
     3121 acacggggag tgagcgtcct tcagcaggaa actggacaga gaaaggtaat ggggtctacg
     3181 tgtcgacctt aacgctggga tctgccgcgg gtcagttgtc tgtgatgccg cgagtgaacg
     3241 gccaaaatgc cgttgctcag ccactggtgc tgaatgttgc aggtgacgca tctaaggctg
     3301 agattggtga tatgacagtg aaggttaata accaactggc taatggacag tctgctaacc
     3361 agataaccct gaccgtcgtg gacagctatg gtaacccgtt gcaggggcaa gaagttacgc
     3421 tgaatttacc gcagggtgtg accagcaaga cggggaatac agtaacaacc aatgcggcag
     3481 gtaaagcgga tattgagttg atatcaaccg ttgcagggga acttgaaatc gcggccgcgg
     3541 tgaaaaactc tcagaagacg gtcacggtga aattcaacgc ggatgccagc accggtcagg
     3601 caaacctgca ggtagacacc gccgttcaaa aagtagcaaa cggcaaagat gcctttacgc
     3661 tgacggcgac cgttgaggat aaaaatggta accctgttcc agggagcctg gtgaccttta
     3721 atctgccccg gggtgtcaag ccgcttacag gcgataatgt ctgggtgaaa gccaacgatg
     3781 aggggaaagc agagttgcag gtggtttcag tgactgccgg aacgtatgag atcacggcat
     3841 cggcggggaa tagccagcct tcgaatacgc agactataac gtttgtagcc gataaggcta
     3901 ccgcaaccgt ctccggtatt gaggtgatgg gcaactatgc gctggcggac ggcaaagcca
     3961 aacaaacgta taaagttacg gtgactgatg ccaataacaa tttggtgaaa gatagcgaag
     4021 tgacgctgac tgccagcccg gcttcgttaa acctggaacc gaatggcact gcgacaacga
     4081 atgagcaagg gcaggctatt ttcaccgcta ccactactgt tgcggcgata tacacactca
     4141 aggcgcaagt gagtcagacc aacggtcagg tatcaacgaa aactgccgaa tctaaattcg
     4201 ttgcggatga taaaaacgcg gtactcaccg catcatctga tatgcaatct ctggtggcgg
     4261 atgggaaatc gactgcgaag ctggaggtga cactgatgtc ggcaaacaac cccgttggcg
     4321 ggaatatgtg ggtcgacatt cagacgccgg aaggggtgac ggagaaggat tatcagttcc
     4381 tgccgtcgaa aaatgaccat ttcgtgagcg gaaaaatcac gcgtaaattt agtaccagca
     4441 agcctggtgt ctatacgttc acatttaacg ccctgaccta tggcgggtac gaaatgaagc
     4501 cagtgacggt gaccattacc gcggtggatg ccgatacggc aaaggacgag gaggcgatga
     4561 aataatttta agaatatgaa agtaacattc taataaacaa taatggcgtt gacatcttca
     4621 acgccattat ttattgaatt tcctgacagt agtctcgtta taaattcatt tagatatagc
     4681 gaaaactaaa ccggacgaac ggggataaaa atttcgcaaa caacatcatt gctatccttt
     4741 tctacgaaat cttcattgta ataaaaccgc tcaatatcac tgccgttcac tctggctaac
     4801 cccctggcgg gtaattccat cagatagagg tttcgcgccc attcagggta gtgctcccac
     4861 tcaccttcat agcggaaaga ggcatacagt cctcggttaa ttgtccactc tttcggcatc
     4921 tctgaatcgg ttggtttgca taccgtacca gcaaaagttt caaccgtgag caaatcgacc
     4981 gattttgttg acggttcaaa cgtggaaacg aaatagatat ccgtcttaaa attcttcata
     5041 catctggcta aatgatgacg tcgtagtttg acttcttcat ccggtgattt attcgacact
     5101 tcaatgtcat atttaaatga gttgccaaaa ataggtgtct caggaaagtt gattaatcta
     5161 cactccgtct tttgctgttg acgtattaaa aaagaaggga agatatttgc gagatcccaa
     5221 taatcacggt ggcggtattc acggggagag cagccgaata actttttaaa ttcgcggctg
     5281 aatgactgct gtgaatcaaa atgcaaagag agtgcaatat caagcataga tttcgcggtg
     5341 agccggacaa gaatggcggc acgacaaagc cttcgtttgc gaatgtattc tcccaaaggc
     5401 acatgcatga aatttcggaa cagaagctgg atgttgcgtc tgctgtagcc agatttctgt
     5461 gcgatatctt cgattgaaat agggtgctca agattgcact caatccactc caggagctgc
     5521 tgtagaatct tctgcctgat catctcattc tccgttaatt ctttgtcata tcgcacattt
     5581 tcgttatacc ctgggcccta agtgtatcaa aaatagcaag ggcgaaggca aaagcactgg
     5641 tagcgtctgc ttcgttgtcc ccaaaaatag agaggtttca gtcctacatt attaatgaat
     5701 tttttgcata agtgatatca aaatccacgt actaatttga ggttacgttt taacgtagac
     5761 tcattgttca tgcctaatgg agggactgac agtggaattt tccgtattaa gtaacaatct
     5821 gaaaatgccg atgatgggat ttggtgtttt tcaggttacc gataaaaacg tgtgcaaaca
     5881 gtcagtgttg aacgctatcc gcacaggtta tcgactcatc gacactgcag cggtatacgg
     5941 taatgaagat gccgtcggtg aagccgttcg tgaagctata tctgaaggtt tatgtacccg
     6001 agatgagtta tttataacgt ctaaattgtg ggtgcaggat atgttgaatc aggatacggc
     6061 agcagcaggt attgaagcat cattaaaaaa atcgggacta gagtacttcg acctctattt
     6121 attgcaccag gctatgcgcg attatttcag tgcgtggcgt gcactcgaag atgcctatga
     6181 agagggcaaa ttaaaagcaa ttggggtttc caatttctat cctcatgttc tggcgaactt
     6241 ttgtgaaacg gtaagagtta aaccgatggt caaccaggtc gagttgcatc cctattttgc
     6301 ccaaccagag gcgctggcaa ccatgaagta ttataacgtg cagcctgaag catgggctcc
     6361 gttaggtggt ggacgacata aaccctttga aaataatctg cttcagagta ttgcagatgc
     6421 ccatcaaaaa tcgatttctc aagtcattct gcgttggaat attcaacggg gagtggtcgt
     6481 tattccgaaa tcgacacatc aacagcgtat cgaagaaaat tttgctatct gggatttctc
     6541 actgacagag aaagaaatgg cacaaattag ttcgcttgat ttgggttatg ttggggagtc
     6601 ggtaaaacat tttaatcctg aatttgttcg tggttgtctt gctgtaaaaa tacatgattg
     6661 atattaatca tatattttac ctgagacgac aagaatcttt taacagggga gtgatattga
     6721 tcttcactct gtcatatctc cggtaatatg gcgtcaggct tcatcatcag aaagggggcc
     6781 tgacgcctga aaaagtgaac aacagacagt gttcggatta tcaatattca gtttttaacg
     6841 ttgaactgga ttcattactg cgttgtttaa gaatttcccg cgccgaatct gccattatca
     6901 tgacagcacc tgccagcatc agagtatctt tcaataccag gcgaccagca ccggataaat
     6961 aagggaaacc atgatgagcg tcacccaatg cgggtaccca tgcctccggg gtggtgatta
     7021 aaaatgagag tgttaccagc ggcgtggtaa atgccatcag tcctcccaat aaacctaacc
     7081 agcgattgac aggattagcc aaaaccagca gagcaataat cacctccacg acgccaagac
     7141 cgttggaaaa accataggta ttattggccg tttgccatgc ccttgcttct ggtttgtatt
     7201 cgccttcgtg agtcagatac tgtttatagt cttccgggtg ttcataaaag aacgacatta
     7261 gtggactgtt tgcgacgaat ggtgtaatgc tgtctgcctc gtaagggaca aactttaata
     7321 acccaatcca cataaaaaca attgcaatac tcagacgaat caatgtcagg ccaattttat
     7381 ctccccgact taacaggtgc aggtattttt ccattgtgaa catccttatt tatacaatac
     7441 tgctgttgcg gttgaattat tatcaattcg catcgatgtg actttccagg atgaagcatt
     7501 atattttctg gctatatcag caatggtatc ctccagaact gtcggtgatg ctgatactga
     7561 cgagacgctg atatgtttca gaattatttt atcatctgca ttggcggaaa atgccataac
     7621 gccagataat agtgttgcaa
//
LOCUS       DAPHDW010000184         7474 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.187, whole genome shotgun sequence.
ACCESSION   DAPHDW010000184 DAPHDW010000000
VERSION     DAPHDW010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7474
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.187"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(93..916)
                     /locus_tag="RH057_004071"
                     /pseudo
     CDS             complement(93..916)
                     /locus_tag="RH057_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414735.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
     gene            complement(970..2688)
                     /gene="proS"
                     /locus_tag="RH057_004072"
     CDS             complement(970..2688)
                     /gene="proS"
                     /locus_tag="RH057_004072"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HDV3130360.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(2800..3507)
                     /gene="tsaA"
                     /locus_tag="RH057_004073"
     CDS             complement(2800..3507)
                     /gene="tsaA"
                     /locus_tag="RH057_004073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HDV3130361.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIAPYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKDSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(3504..3908)
                     /gene="rcsF"
                     /locus_tag="RH057_004074"
     CDS             complement(3504..3908)
                     /gene="rcsF"
                     /locus_tag="RH057_004074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HDV3130362.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKRMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(4026..4841)
                     /gene="metQ"
                     /locus_tag="RH057_004075"
     CDS             complement(4026..4841)
                     /gene="metQ"
                     /locus_tag="RH057_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HDV3130363.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(4881..5534)
                     /gene="metI"
                     /locus_tag="RH057_004076"
     CDS             complement(4881..5534)
                     /gene="metI"
                     /locus_tag="RH057_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HDV3130364.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(5527..6558)
                     /gene="metN"
                     /locus_tag="RH057_004077"
     CDS             complement(5527..6558)
                     /gene="metN"
                     /locus_tag="RH057_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HDV3130365.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            6746..7321
                     /gene="gmhB"
                     /locus_tag="RH057_004078"
     CDS             6746..7321
                     /gene="gmhB"
                     /locus_tag="RH057_004078"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HDV3130366.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
BASE COUNT         1837 a         1931 c         1897 g         1809 t
ORIGIN      
        1 catataatgc aacattttga attagcacga ttttgtaggc cggataaggc gtttacgccg
       61 catccggcat tttgtgcacg aggctggctt tatcactgag tcagcccaac aaagcgaccc
      121 gccttgatat aaatcccagg cttcaggtgc ccaacatact gtaactgcgt ttctggctta
      181 aacgcagaga catcaccaat acgcatatgc atcagatcgg cagggctaat ccagcccgat
      241 tgcgccagtg taagtggatg cccggctttg gcaaaggcat ccgtgacaaa ttccgaacaa
      301 aaccacgact ttttgtctcc ttcgcccaca ctgcttaact gcgctttcgc caggccgctg
      361 acgcactttg gcgaaaatcc tcggagaacg gattcagtga gcacatctgg cgagtcacca
      421 taaagggaat aaattcgaca atgccgcgat agttataacc gctatctttg attttattgg
      481 caaacgcggt gatatctgtg gcttgttgcg gggtaagatc cgggactcgt aagacgaaaa
      541 gcttatcact atgcttcatc gcttttttaa gggaaacaat ctggacgcca gcgcctgtcg
      601 cttctgcaac gttattatca cccagaaaga ttgcaacgtg actcacagag gaagtgctga
      661 agacgcggat tccaaatgag gttaccccaa ggcttgagga gaacagcaaa tcgccgggtt
      721 tgagatcagg tgccgttatt tcttttattg attgttcggt gaaagagctt tgatgctgga
      781 atttgacagc ccatgttttt gcctcggcat ctactgctgt ggctgatggg tctggctggc
      841 tgatatcaac ggtacaagcc gaaagtaaaa gaaagctggg gagaaacagg cggcagtacg
      901 cctttggttt atccatttta tacaatccat gtaaaaaaag ggccctgaaa ttcaggaccc
      961 tttctggcat cagcctttaa tctgtttcac cagatattcg acgatgtcac cagtcttaat
     1021 taactgtttc tcgccgttgc gacgatattt atattcgata tcgtcgttgt cgaggttacg
     1081 gtcgcccagc acaatagtgt gcggaatacc gatcagttcc atatcagcaa acatcacgcc
     1141 cggacgctct ttgcggtcat ccagcagcac ttcgatacct tgtgcacgca gttcgctgta
     1201 cagtttctca gcaagctctt gtacgcggaa ggatttgtgc atgttcatcg gcagaatcgc
     1261 cacctggaac ggcgcgatag cgtcaggcca tacgatgcct cgttcgtcgt agttctgctc
     1321 aatcgccgca gctaccacac gcgttacccc gataccgtag caacccatcg tcaggatttg
     1381 gttacggcca tcttcaccct gtacggaggc tttcagtgct tcggagtact tggtacccag
     1441 ctggaagatg tgaccaactt cgataccacg tttgatcagc agcgtaccct ggccatccgg
     1501 gcttggatcg ccagccacca cgttacggat atctgcaact tccggggtag cgacatcgcg
     1561 atcccagttg atgccgaagt agtgtttacc atcgatgtta gcaccagcag cgaaatcact
     1621 catcgccgca acggtacggt caatcaccac cggaatcggc atgtttaccg gacccagtga
     1681 acccggaccg gctttaacca cggcacgaat ttcttcttcg gtcgcgaaag tcagcgggct
     1741 tgcaacctgc ggcagttttt ctgctttaac ttcgttcagc tcgtgatcac cgcgcaccag
     1801 cagcgcaacc agcgggaagc tactgccttc aaccgcttta accagcagag tcttaaccgt
     1861 tttctcaatc ggcagattga actgttcaac cagttccgcg atggttttcg cgttcggcgt
     1921 atcaaccagc gtcatttcct gggtagcagc agcgcgcggt tctttcggcg cgatagcttc
     1981 tgccagttca atgttagctg catagtcaga ggtgtcggag aagaccacat cgtcttcacc
     2041 gctctgcgcc agcacctgga attcgtgaga ggcgctgccg ccgatagaac cggtgtcggc
     2101 ttgtacggcg cggaaatcca gccccatgcg gctgaagatt ttgctgtagg ccgcatacat
     2161 tgcatcgtag gtttcctgca gggattcctg agaagtatgg aaagagtaag catctttcat
     2221 caggaattcg cgggaacgca tgacgccgaa acgcggacgc acttcgtcgc ggaacttggt
     2281 ctggatctga tagaagttca gcggcagctg tttgtaagag ctaagctcgt tacgaatcag
     2341 gtcagtgata acttcttcat gagttgggcc gagtacgaac ggacgctcgc cacggtcaac
     2401 aaaacgcagc agttccggac cgtactgttc ccaacgacca ctctcttgcc acaaatcagc
     2461 tggctgaacc accggcatcg acacctcgat cgcaccggcg ttgttcatct cttcacgcac
     2521 gatgttttcg acttttttca gaacgcgcac gccggtcggc agccaggtat ataacccgga
     2581 ggccagcttg cggatcatcc cggcgcgcag catcagctga tggctgatca cctcggcgtc
     2641 ggcaggtgtc tccttgagag tggagagcag gtattggcta gtacgcatgt tgttacggtt
     2701 ccagttggaa ggtagaacag gctcaaggcg agcctgggac aaaaaaaagt gatttagttt
     2761 accagtgcaa aagaaatgtc aaaagagaag ggcgtgaatt taacgcggtt ccagcgcaaa
     2821 gacttcaaaa cctgcgtcgg tgacgcgcca gcgaacgtta aaatcatgta gccagacggc
     2881 ataggttttg cccgtttcct cacctttacg ataggccggg cgtgggtcct gcgccagtac
     2941 ttcgcggata aacagcgtta actgcggata acgcttctcc agcgtcaaaa gctgcttttc
     3001 gacctctgcg gtaaaactca ccgccatctc tgccgctggc gcactttgcg catagctggc
     3061 actggcatcg ggaagcgatt cggcaaaggg gagatacggt ttgatatcca ctaccggcgt
     3121 accatcgacc agatccagac tgccgagctt cagaatcacg ctgtctttat ggcaaacaac
     3181 ctctttcagc tctaccagcg acataccaat tgggttaggg cggaaagtag agcgtgttgc
     3241 gaaaaccccc attctggcgt taccgccgag gcgcggtgga cgcacagtcg gacgccagcc
     3301 gccttccatc gtttgatgaa agacgaaaag gatccataaa tggctgaacg cttccaggcc
     3361 gcgaacggcg tcggcctggt tgtagggagc aatgagatgc agttcgccgt tggcgctttt
     3421 taccagacct ggctggcgcg gaacggcgaa tttttcttta tagggcgagc gaataacgcc
     3481 tatttgctcg aactggaaac tgctcatttc gccgtaatgt taagcgcaga gccgatacat
     3541 acagcctgac gatagcagcc tggcgtaccg ctggtgactt cgcagctatg cagtaatacc
     3601 gcattggcct tcattttcga ggcgttgatt tgcatccgct tacgcgcggt tggaatgctc
     3661 ggcggagagt cctgattaga ggcctggcaa gagtcgccac tgacttcacc gagatcgcgg
     3721 aacggtttgc cgactaattc ttctgcattg gtataaattc ggaccggcgt ggcgcgcggc
     3781 gctttcggtt ttgcaggctc cgctttcggc tggggtgcag tgctttgaac gggttcgaca
     3841 ggggatctgc ttaacatgga acagccgctt agcatgagtg ctactaaaca gatcggtaaa
     3901 gcacgcatag tatttcctca atgtatgatc aaaacgtcaa tattgaatca ggagcttgta
     3961 aaaatgacaa gacgggcaag cgcccgtcct gaatgatatt acaaattgtg gaaacagcct
     4021 aaaaattacc agcctttaac agcgccgccg ttaaacactt tgtttgctgc ttcgtaaact
     4081 tcatcagact gataagcctg gacgaatttc ttcacgttct cggcgtcttt gttatcttca
     4141 cgcgttacga tcaggtttac gtacggggag tctttatctt cgacaaagat accgtctttc
     4201 gctggagtca ggccaatctg gctggcatag gtggtattga taactgccag agcgatttgc
     4261 gcgtcgtcca gagatcgagg cagctgcggt gcttccagct caacaatttt cagatttttt
     4321 gggttctcaa caacatcaag aacggtcggc agcaggccaa cgccatcttt cagtttgatc
     4381 aagcccactt tttgcagcag cagcagtgaa cgaccaagat tagttggatc gtttggcacg
     4441 gcaacctgcg aaccatcctg cagttcatcc agtgatttga ttttcttgga gtaaccagca
     4501 atcggataaa caaaagtgtt gcctactgcg accagtttgt agccacgatc tttcagttgc
     4561 tgatcaaggt acggtttatg ctggaaggcg ttggcgtcga tatcgccttt gctcaatgct
     4621 tcgtttggca gaacatagtc gttgaaggtt accagctcaa cgtccaggcc atatttgtct
     4681 ttcgcaactt tctgcgcgac ttctgctacc tgctgttcgg caccaacaat cacgccgact
     4741 ttaatgtggt ttggatcttt ttcatcctga ccgcagccta ccagtgccag tgatccaatc
     4801 agggctccca ctgccgcaaa ggttttgaat ttgaacgcca taccttattc cttcttcaat
     4861 tatttatgtt gtgttgaacg ttacttgcga gtgacagccc ggacgatgcg gtcgcctgcg
     4921 aactgaatta aataaaccag aatgaccagc aataccagta ccgtattcat caccgtcgcg
     4981 ttatagccga tgtagccata ctgatagcca atctgaccta aaccaccggc accgactgca
     5041 ccacccatcg cggaataacc gaccagggta atcagggtga tagttgccgc attcaccaga
     5101 cccggcaatg cttccggtaa cagcacttta cggacgatct gcatcggcgt ggcacccatt
     5161 gcacgggaag cttcaattaa cccggttggg atctccagca gagcgttctc gaccatacgg
     5221 gcaataaacg gtgctgcacc aacggttaac ggaacaatcg ctgcctgcaa accaatcgat
     5281 gtaccgacaa taacgcgggt aaacggaatc atccatacca gcaagataat gaacgggatg
     5341 gaacggaaaa tgttcacaat cgcagaaacg gtacgataca gcttcgcgtt agcaataatt
     5401 tgccccgggc gcgtaacata aagcaggacg ccgaccggca gaccaatcac aaagccaaaa
     5461 aatccggata cgaaggtcat tgccagcgtt tcccatacgc cacgaaccag caaccacatc
     5521 atcggctcag acataaccca gtacctctac ttttacatgg tgttcctgca gccaggcaat
     5581 ggcggcttgc gtatcttgtt gtgtgccgtg catttcagtc agcatgatgc cgaacttaac
     5641 gccaccggcg taatccatct gcgcgctaat aatgttgttg ttgacgttga aacgacgcgc
     5701 ggtttcagaa agcagcgggg catcgaccga ttgaccggta aactccaggc gcaacatggg
     5761 cacgcagtca gtaaatggct ccgcttgcag acgttcctgg taatcttccg ggatatccag
     5821 atgcagggtc gactgaataa acttctgcgc cagcggcgtt ttcggatgcg agaacacttc
     5881 acttaccgtg tcctgctcga tcagttcgcc attgctgatg accgccacgc aatcacaaat
     5941 acgcttcaca acgtccattt cgtgggtgat caacagaatc gtcaaaccca gacggcggtt
     6001 aatgtctttc agcagttcga gaatagaacg tgtcgttgcc gggtccagcg cgctggtggc
     6061 ttcatcacac agcaatactt tgggattgct ggctaatgca cgggcaatcg ccacacgttg
     6121 tttctgccca ccggaaagat tcgacgggta gctatcatgc ttatcgccaa gaccaaccaa
     6181 tgacagcaat tccgtcacgc gacgtttgat ctcgtctttc ggtgtgttgt ccagctccag
     6241 cggcagagcc acgttgccaa aaacagtacg cgaagagagc aggttaaaat gctggaaaat
     6301 cataccaatc tggcggcgag ctttggtcaa ctcggattct gacagcgtgg tcagttcctg
     6361 gccatcgacc agcacgctac cctcggttgg gcgctccagc aggtttacac aacgtataag
     6421 cgtactctta cccgcgcctg aggcaccgat aacgccataa atttgtccag ctggcacatg
     6481 caggctgacg ttgttcaacg cctggatggt gcgggtgccc tggtggaaca ctttggtgat
     6541 attcgaaagt tttatcattg attatttatt atcgtcatta agttagtcgt ggcatctcga
     6601 atgcctgaaa cgggcaacgc cgtcaatgaa atggatgtta aggcatccag acgtctaaat
     6661 caatcaggtt tatgcgaaga gcactttctt gcaggtcgaa acatgcgata ctagcgtccc
     6721 atgccttatt aaggagctat aaaaggtggc gaagagcgta cccgcaattt ttcttgaccg
     6781 tgatggcacc attaatgtcg atcacggcta tgtccatgag atcgacaact ttgaatttat
     6841 cgacggtgtt attgacgcca tgcgcgagct aaaaaaaatg ggctttgcgc tggtggtagt
     6901 aaccaaccag tctggcattg ctcgcggtaa atttaccgaa gcacagtttg aaacgctgac
     6961 cgagtggatg gactggtcgc tggcggaccg agatgtcgat ctggatggta tctattattg
     7021 cccgcatcat ccgcagggta gtgttgaaga gtttcgccag gtctgcgatt gccgcaaacc
     7081 acatccgggg atgcttttgt cagcacgcga ttatttgcat attgatatgg ccgcttctta
     7141 tatggtgggc gataaattag aagatatgca ggcagcggtt gcggcgaacg tgggaacaaa
     7201 agtgctggtg cgtacgggta aacctattac acctgaagca gaaaacgcgg cagattgggt
     7261 gttaaatagc ctggcagacc tgccgcaagc gataaaaaag cagcaaaaac cggcgcaatg
     7321 attaaaagat gagcggttga aataaaaatg catttttccg cttgtcttcc tgagccgact
     7381 ccctataatg cgcctccatc gacacggcgg atgtgaatca cttcacacaa acagccggtt
     7441 cggttgaaga gaaaaaatcc tgaaattcag ggtt
//
LOCUS       DAPHDW010000185         7236 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.188, whole genome shotgun sequence.
ACCESSION   DAPHDW010000185 DAPHDW010000000
VERSION     DAPHDW010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7236)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7236)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7236
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.188"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(121..756)
                     /gene="msrQ"
                     /locus_tag="RH057_004079"
     CDS             complement(121..756)
                     /gene="msrQ"
                     /locus_tag="RH057_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HDV3130367.1"
                     /translation="MRLTVKQVTWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLATLLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQAMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLRSLFNRLRKQVHNKLSV"
     gene            complement(757..1761)
                     /gene="msrP"
                     /locus_tag="RH057_004080"
     CDS             complement(757..1761)
                     /gene="msrP"
                     /locus_tag="RH057_004080"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
                     /protein_id="HDV3130368.1"
                     /translation="MKKNQFLKESDVTAESVFFMKRRQVLKALGISAAALSLPHAAHA
                     DLLSWFKGNDRPPAPAGKPLEFSKPAAWQNNLPLTPADKVSGYNNFYEFGLDKADPAA
                     NAGSLKTDPWTLKISGEVAKPLTLDHDDLTRRFPLEERIYRMRCVEAWSMVVPWIGFP
                     LHKLLALAEPTSNAKYVAFETIYAPEQMPGQQDRFIGGGLKYPYVEGLRLDEAMHPLT
                     LMTVGVYGKALPPQNGAPVRLIVPWKYGFKGIKSIVSIKLTRERPPTTWNLAAPDEYG
                     FYANVNPHVDHPRWSQATERFIGSGGILDVQRQPTLLFNGYADQVASLYRGLDLRENF
                     "
     gene            complement(1870..2283)
                     /gene="hiuH"
                     /locus_tag="RH057_004081"
     CDS             complement(1870..2283)
                     /gene="hiuH"
                     /locus_tag="RH057_004081"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HDV3130369.1"
                     /translation="MLKRYLVLSVVTAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            2416..3087
                     /gene="hprR"
                     /locus_tag="RH057_004082"
     CDS             2416..3087
                     /gene="hprR"
                     /locus_tag="RH057_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HDV3130370.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLRMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3087..4445
                     /gene="hprS"
                     /locus_tag="RH057_004083"
     CDS             3087..4445
                     /gene="hprS"
                     /locus_tag="RH057_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HDV3130371.1"
                     /translation="MKRLSITVRLTLLFILLLSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHGDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIVLCSVLSPLLIRTGLREIKKLSGVTEALNYNDSREPVEVSALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQTRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVECNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDTNGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLNKHNVFRITLPQ
                     RN"
     gene            complement(4553..5404)
                     /gene="hchA"
                     /locus_tag="RH057_004084"
     CDS             complement(4553..5404)
                     /gene="hchA"
                     /locus_tag="RH057_004084"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HDV3130372.1"
                     /translation="MTVQTSKSPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFHNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHGDNPLNGYSICAFPDAADKQTP
                     EIGYMPGHLTWYFGDELKKMGMNIINDDITGRVHKDRKLLTGDSPFAANALGKLAAQK
                     MLAAYAG"
     gene            5660..5856
                     /locus_tag="RH057_004085"
                     /pseudo
     CDS             5660..5856
                     /locus_tag="RH057_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353128.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5996..7189)
                     /gene="ompC"
                     /locus_tag="RH057_004086"
     CDS             complement(5996..7189)
                     /gene="ompC"
                     /locus_tag="RH057_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV3130373.1"
                     /translation="MKRKVLAMLVPALLVAGAANAAEVYNKDGNKLDFYGKVAGLHYF
                     SDNSGADGDMSYARIGFKGETQIADQFTGYGQWEYNLQANGTEGDGDNSATRLAFAGL
                     GFGQNGTFDYGRNYGVVYDIEAWTDMLPEFGGDTYAGADNFMNGRTNGVATYRNNGFF
                     GQVDGLNFALQYQGNDKSGGPFGQEGSGSGDGRSLSKENGDGFGMSTSYDFDFGLSLG
                     AAYSNSDRSDRQVGVGLNDRNHSNRNAGGETAEAWTVGAKYDANNVYLAAMYAETRNM
                     TPYGGGEFDNGESRSAIANKTQNFEVVAQYQFDFGLRPSIAYLQSKGKDLGGWAHDGN
                     GDPRYTNKDLVKYVEVGATSYFNKNMSTYVDYKINLLDNDDDFYEANGIATDDIVAVG
                     LVYQF"
BASE COUNT         1967 a         1569 c         1720 g         1980 t
ORIGIN      
        1 attttggtga tttgaggttg atgacaaacg aaacataacc tgatgcgatc tgtgtaggcc
       61 ggatagggcg ctcgcgccac atccggcagg gataacgcgt actttgtcgg caatatgaag
      121 ttatacagat aatttgttgt gaacctgctt gcgtaaccgg ttaaaaaggg aacgcaactt
      181 cttataccgt aaggctaaaa gcagtacagc cagcccagcg tagatgagcg gctgcggtga
      241 aataatcttc acggaccaca gataatgtat tggggccaga atcgcgacaa gatagacgaa
      301 attatgcaac tgttgccagt gcttgccgag ttttcgctgc atcgcctggg ttgaagtaaa
      361 cgctaaagca agcaaaataa cccagctgat aattcctaac gttaaataag gccgggtaat
      421 taactccttg cctaataacg ccagattatt cacgcccagc tccagcaatg cgtaactggt
      481 taaatgcagt gtcgcccagg caaagcacca taagcctaac aggcggcgag tgcgtatcaa
      541 taacggctgt tttgcgtagc gtgccagagg tgtgattaac aaggtcgcca gcaaaaattt
      601 cagtgcagtg cgaccggtaa aatgctgaat atctttcacc ggatcggcac ccagtccacc
      661 gtgattgatc gcccagacca gccagagaaa cggcaacaat ccggcaagat gcaggcaaac
      721 tttcagccat gtcacctgtt ttactgtcag acgcattcag aaattctccc gcaaatccag
      781 gccacgatac agcgatgcca cctggtcggc gtaaccatta aacagtagcg ttggctggcg
      841 ctgtacatcg aggatgccgc ctgaaccaat aaatcgttcg gtagcctgtg accagcgtgg
      901 gtgatcaaca tgcggattaa cgttggcgta aaaaccgtat tcgtcaggcg ctgccagatt
      961 ccaggtggtt ggcggacgct cgcgggtcag cttaatactg actatcgatt taatcccttt
     1021 aaagccatat ttccacggca caatcagtcg taccggcgcg ccattttgtg gcggtaacgc
     1081 cttgccataa acacccacgg tcatcagtgt gagcggatgc attgcttcgt cgagacgcaa
     1141 tccttcgaca taaggatatt tcagcccgcc gccgataaag cggtcctgct ggccaggcat
     1201 ctgttccggt gcataaattg tttcgaaagc gacatacttc gcattgctgg tgggttcggc
     1261 aagcgccagc aatttgtgca gcggaaaacc aatccacggc accaccatcg accacgcttc
     1321 cacgcagcgc atacgataaa tacgctcttc cagcgggaag cgacgggtta aatcatcgtg
     1381 atcgagggtc aatggttttg ccacttcgcc gctgattttc agtgtccatg gatcggtttt
     1441 caggctacca gcattagcgg cgggatcggc tttatccagc ccgaattcat agaagttgtt
     1501 atagccggaa actttatctg ctggcgtcag tggcaggtta ttttgccagg cggcaggctt
     1561 gctgaactcc agcggttttc cggcgggggc gggtgggcga tcgttccctt taaaccagct
     1621 aagcagatcg gcatgcgcag cgtgaggcaa agaaagtgca gctgcgctga tgcccagtgc
     1681 ttttaacacc tgccgacgct tcataaagaa taccgactcg gccgtaacat ctgattcttt
     1741 taaaaattga ttctttttca taagccatca cacttttttg tcgttagcca gcaagagccg
     1801 tttactgtta atctgtcagg gagtgtcctc cctaacagta gcagagatct gcttttacat
     1861 atttatgcat taactgccac gataggttga atacccatat tggctaagta ataaaggcac
     1921 atgataatgc tcgttcactt tattaatatg aaattcaacg gggatctcag ggaagaaact
     1981 ttcaagattt tgtttcttga aatagtcccc ggttttaaat acgacacggt aatcgcccgt
     2041 agttgcagtt tgctcgggcc acagtgcctt aattcgtcca tccttatctg ttttggcggt
     2101 attaagttgt aaccagccgt tgtccgcttt cttttcaaga gtgactgtca cgtcggcagc
     2161 gggttttcct gtttgctggt tcaaaatgtg cacgctaaga atgttttgtt gtgcagcata
     2221 gaccaaagaa ggtaatgaaa atgcagccgt tactacggag agtactaaat aacgctttaa
     2281 catgtttata tccttgtcat gtgaatgagt tttgaggata gcgaaaaagc gtccagggta
     2341 accttacagc agcattacaa ttttgtaatg aaccaggctg tttctataac atatgattta
     2401 tggcatatta ttttcatgaa gattttactt attgaagata atcaaaggac ccaggagtgg
     2461 gtaacgcagg ggctttccga agcgggttat gtcattgatg ccgtttctga tggcagagat
     2521 gggctttatc ttgcgctgaa ggatgattat gcattgatca ttctggatat tatgcttccg
     2581 ggtatggatg gctggcagat cttacaaacg ctaagaacag caaagcaaac ccctgttatt
     2641 tgccttactg caagggattc tgtcgatgac agagtcagag ggctggacag tggggcaaat
     2701 gattatctgg taaaaccttt ttcattttct gagttgctgg caagggttcg ggcacaatta
     2761 aggcaacatc acgctttgaa ttcaacatta gaaatcagcg gcttaagaat ggactctgtt
     2821 agtcaaagtg tgagcaggga caatatcagt attacactga cgcgcaagga gtttcagtta
     2881 ctttggctac tggcctccag agctggcgaa attataccca gaacggttat tgcgagtgaa
     2941 atttggggaa tcaactttga tagtgatacc aatacggtgg atgtcgccat tcgcaggctc
     3001 cgcgcaaaag ttgatgatcc ttttcctgaa aagctaattg ccacaatccg ggggatgggg
     3061 tattcattcg tagcggtaaa aaaataatga aaagattatc tataaccgtc cgtttaacct
     3121 tgctttttat attgctgctg tctgttgctg gcgctggaat tgtctggact ctctataatg
     3181 gcctcgcaag tgagttgaaa tggcgcgatg atacaacact cattaaccgg acagcgcaga
     3241 tcaagcagtt gttaattgat ggggtaaatc cagatacgtt acctgtatac tttaaccgga
     3301 tgatggatgt tagtcaggat atcttgatca ttcatggtga tggcatcaat aaaattgtta
     3361 accggacaaa tgtcagtgat gacatgttaa ataacatacc tgctagtgag acaatcagcg
     3421 cagctggcat ttacagaagc attattaatg atactgagat tgatgcttta cgaattaata
     3481 ttgatgaagt ttcgccatca ttaacggtta ctgtggctaa attggcttca gccagacata
     3541 acatgcttga acagtataaa attaatagca ttataatttg cattgtcgcc attgtacttt
     3601 gctcagtatt aagtccgctg ttaatcagaa cgggattacg agagatcaaa aagttgagtg
     3661 gtgtaacgga agcgctgaat tataacgata gccgagagcc tgttgaggtt agcgcattac
     3721 cgagagaact aaaacctctt gggcaggcgt tgaataaaat gcatcatgct ttagtcaaag
     3781 attttgagcg tctaagtcaa tttgctgacg atctcgctca tgaacttaga acgccaatta
     3841 atgcattact gggtcagaat caggttacgc tcagtcaaac cagaagtatc gctgaatatc
     3901 aaaaaacaat tgccggtaac attgaagagc tggaaaatat ttcgcggtta acagagaaca
     3961 tactgtttct tgccagggca gataaaaaca atgttttggt gaaactggac tcgctttctc
     4021 tcaataagga agtcgaaaat ttgttggact atcttgaata tctttcagac gagaaagaga
     4081 tttgctttaa ggtcgagtgc aatcagcaaa tctttgcgga taaaatttta ctgcaacgaa
     4141 tgttatcgaa tcttattgtt aatgccatta gatattcacc agaaaaatcg cgtattcata
     4201 taaccagttt tcttgatacc aacggatctc ttaatattga tatcgccagc cctggaacga
     4261 aaattaatga gcctgaaaaa ctcttccgta gattttggcg gggagataat tcgcgtcatt
     4321 ccgtaggtca gggactaggc ctttctttag tcaaagcgat tgccgaatta catgggggaa
     4381 gtgctacgta tcactatctc aataagcata atgtgttccg gattacgttg ccgcaaagaa
     4441 attaacatag ggcttcagta cgccataaat taaccgtgaa aaaacgcagc cacgtcgtga
     4501 gtactaacgc ggccgcgatt gattatgcgc ttacattcaa acgtaacagg gattaacccg
     4561 cgtaagctgc caacattttc tgcgccgcca gtttacccaa cgcattcgct gcaaaaggac
     4621 tgtcgccggt gagaagttta cggtccttat gtactcgccc ggtgatgtcg tcattaatga
     4681 tattcatgcc cattttcttc agttcgtcgc cgaagtacca ggtgagatga cccggcatat
     4741 agccaatctc tggcgtttgt ttgtctgcgg cgtctgggaa tgcgcaaatg gaataaccat
     4801 tcagtgggtt atcgccgtgg cgaagcgcca gaaaagccgc cgggccgtgg caaagggaga
     4861 taacaaaacg gtcatttttg attgcccact gtaaagcggc agccacgtcc tggctttcag
     4921 gtaaaccaat aagtgcgcca tgaccaccag gaacaaagat tgctgcatat tcgctatcag
     4981 cgttgaggct ggcgacgaca tccgcgagtt tcttcggatt gtggaacaac gatttatgct
     5041 gctcaaagaa tggcattact ttttcatctt tgtgcggcat agcccagtat tcaaacttgg
     5101 tcatcagacc ggaaatggtc gccacttcga attcgaaacc tgcagcatgg agatgataca
     5161 acggcagcaa cgtttcaatc ggatggttac cggtcgagaa cagttttccg ttatcggtcg
     5221 gcaaataacg ttcgtccgcg gcgatcacca gaattttatg tttaccgcga tacggttttg
     5281 gatagtccac gccatccaga tcagagacag gactggtata ttggctaagc gaatattctg
     5341 aagggaagaa ggcattatct tcagcaatat cgacctgcgg acttttactt gtttgaacag
     5401 tcatagtgta ttccttattg ttgcttagtt agggtagtca ctatatttgc gactgagctg
     5461 gtcattattt ttaaccaggc tggaaaattg agtcaatata tcgaaagcgg tagtcggcgg
     5521 gtgtaagtat gtaatgatga tgcttcactg gtgttgaata ttattcacgc caaattttcc
     5581 ttattgagca acgtcgtcgc aagtggcctc aatgatttta gaccattatc agaggtacgg
     5641 ggcggcgggg agagatttag tgctgtggaa tattgtggtg ctgatgaata tttatatccc
     5701 ggattggtgg agaaaaagct ttttccatgc actttttttg caaatatttc tatatgggaa
     5761 gcgtgtttgt tacggattaa ttcataaaga aatgcatttg ggtgttggct tgaagagtcg
     5821 ctggttgatg ttattgtgcc aggaggggca gggtaagggg gtgtatcgat aagctttggc
     5881 aattgcgtgt gccgcagatg gagatgaaat gccaataaaa aagcctgagt tcgatgaggg
     5941 acgaactcag gctagtgtta agttttacag taacacgccg cggcgtgttg gctgattaga
     6001 actggtagac taagcccaca gccacgatat catcggtagc gatgccgttt gcttcgtaga
     6061 agtcatcgtc gttgtccagc aggttgattt tgtaatcaac gtaggtggac atgtttttgt
     6121 tgaagtagga ggtcgcaccg acttcaacgt atttaaccag gtctttgttg gtgtaacgcg
     6181 gatcgccatt accgtcatga gcccaaccgc ctaaatcctt acctttagat tgcaggtaag
     6241 cgatggacgg gcgcaggcca aagtcgaact gatactgagc aacaacttca aagttttggg
     6301 ttttgttagc gatggctgat cttgattcac cattatcaaa ctcgccacca ccatatgggg
     6361 tcatattgcg agtttcagcg tacatagctg ctaaatacac attattagca tcatattttg
     6421 caccaacagt ccatgcttca gcggtttcac caccagcatt tctgttgcta tggttacgat
     6481 catttaaacc aactccaacc tgacgatctg aacgatcaga gtttgaatat gcagcaccca
     6541 gacttaaacc gaagtcgaag tcgtaggatg ttgacatacc gaaaccatca ccattttctt
     6601 tggaaaggct acggccatca ccactaccgg aaccttcctg cccgaatggt ccaccacttt
     6661 tatcattacc ctgatattgc aaagcaaagt tcaagccatc aacctgacca aagaagccat
     6721 tgttacggta ggttgcaaca ccgttggtac gaccgttcat gaagttgtcg gcacccgcgt
     6781 aggtatcgcc accaaattct ggcagcatat cggtccatgc ttcgatgtca tatacgacac
     6841 cgtagttacg accataatcg aaagtaccat tctgaccaaa acctaaacct gcgaatgcca
     6901 gacgggtagc agagttatct ccatcgcctt ctgtgccatt tgcttgcagg ttgtattccc
     6961 actgaccgta accagtgaac tggtcagcga tttgtgtttc gcctttgaaa ccgatacggg
     7021 catatgacat atcgccatca gcgcccgagt tgtcagagaa gtagtgcaga cctgctactt
     7081 ttccgtagaa atccagttta ttaccatctt tattatagac ttcagccgca tttgctgcgc
     7141 cagcaactaa taacgccggg accagcattg ccagaacttt tcttttcatt atgtattccc
     7201 ttgtgattat aatcttcatg aatatatcaa taagtg
//
LOCUS       DAPHDW010000186         7152 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.189, whole genome shotgun sequence.
ACCESSION   DAPHDW010000186 DAPHDW010000000
VERSION     DAPHDW010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.189"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            339..1376
                     /gene="dusA"
                     /locus_tag="RH057_004087"
     CDS             339..1376
                     /gene="dusA"
                     /locus_tag="RH057_004087"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HDV3130374.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIERELSQGTYLGHITRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            1510..1752
                     /gene="pspG"
                     /locus_tag="RH057_004088"
     CDS             1510..1752
                     /gene="pspG"
                     /locus_tag="RH057_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HDV3130375.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKVPKYQRYDRWRY"
     gene            complement(1918..2901)
                     /locus_tag="RH057_004089"
     CDS             complement(1918..2901)
                     /locus_tag="RH057_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HDV3130376.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDRVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGVGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            2984..4399
                     /gene="dnaB"
                     /locus_tag="RH057_004090"
     CDS             2984..4399
                     /gene="dnaB"
                     /locus_tag="RH057_004090"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HDV3130377.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            4452..5531
                     /gene="alr"
                     /locus_tag="RH057_004091"
     CDS             4452..5531
                     /gene="alr"
                     /locus_tag="RH057_004091"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HDV3130378.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            5784..6977
                     /gene="tyrB"
                     /locus_tag="RH057_004092"
     CDS             5784..6977
                     /gene="tyrB"
                     /locus_tag="RH057_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HDV3130379.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLQAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEATNGVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     GAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
BASE COUNT         1684 a         1763 c         1891 g         1814 t
ORIGIN      
        1 aaggcattac ataaaagaac ctaatactta ttgggttctt ttttcttcta tcagtgccat
       61 tagcaggaag agatatcacc ggagtttaat gtgtgatttt ttatttatcg tcgaacctgg
      121 attgtttatc attgtcctta acaaggctaa cggctaataa gattatttcc atcacttcgt
      181 gagagcttca tgccttgatt tgatctcaat tttcttttgc aatgagacag gcgcttcctg
      241 ttgttatggt atagtacccc gctattgagc ctcctgaaca gtgatgctga ataacataac
      301 ccaatgatat atcgataaaa taatctctac atttgaaaat gcacggtaat tctgaaatgc
      361 aaaaaatcaa ccaaaccagc gcaatgcctg aaaaaactga cgttcactgg agtggtcggt
      421 ttagcgttgc accaatgctc gactggacgg acagacattg ccgctatttc ttgcgtctgc
      481 tttcccgcaa tacgttgctg tataccgaaa tggtgaccac aggggcgatt attcacggta
      541 aaggtgatta cctggcgtac agtgaagaag aacatccggt agcgttgcaa ctcggcggta
      601 gcgatccggc ggcgctggca cagtgtgcga agctggcaga agcgcgtgga tatgatgaga
      661 tcaacctgaa tgtcggctgc ccgtctgacc gggtgcagaa cggcatgttt ggtgcgtgtc
      721 tgatgggtaa tgcgcagctg gttgccgact gtgtgaaagc gatgcgcgat gtggtgtcga
      781 ttccagtgac ggtgaaaacg cgtattggca tcgatgacca ggacagctat gaatttctct
      841 gcgatttcat caacactgtt tccggcaaag gcgagtgtga gatgtttatc atccacgcac
      901 gtaaagcctg gctttcgggg ttaagcccga aagaaaaccg tgaaatcccg ccgctcgatt
      961 atccgcgtgt gtatcaactg aagcgtgact ttccgcatct gacaatgtcg attaacggtg
     1021 gtatcaagtc gctggaagag gccaaagcac acctgcaaca tatggatggc gtgatggtcg
     1081 ggcgcgaggc gtatcagaat ccgggtattc tggcggcagt agaccgggag atctttggtt
     1141 cctcggatac cgatgccgat ccggtggcgg tagtgcgcgc catgtatccg tacattgagc
     1201 gtgaactcag ccaggggacg tatctcggcc atattacccg gcatatgttg ggcttgttcc
     1261 agggtattcc tggcgcgcgg cagtggcggc gttatttaag tgaaaatgcc cataaagcgg
     1321 gtgcagacat taatgtgctg gaacacgcgc tcaaactggt ggcggataag cgttaacttt
     1381 tcaccaaaaa gtagtcaaat tcaccacgcc ctgcgcaccg tcgcggggcg ttttgctgtt
     1441 aaatcaatag attatttttg gcatgattct tgtaatgcca gcaagagatt tcatatttgg
     1501 gagagcatca tgctggaact actttttgtg attggctttt ttgtcatgct gatggtcacc
     1561 ggcgtttcgt tgctgggcat tatcgccgcg ctggttgtgg cgacggccat tatgttcctc
     1621 ggcggtatgc tggcattgat gattaagttg ctgccgtggt tactactggc gattgcggtg
     1681 gtgtgggtta ttaaggcgat taaagcacca aaagtgccga aatatcagcg ttatgaccgc
     1741 tggcgttact aagggattgt gcggatgatc acaacctaag gttttatcct tagaacaaaa
     1801 taggaattga taatcaaatc tgtcactatt gcgcctctaa cagattcatc gtgctgtacc
     1861 ctacatacag ccgaactata aaaagaaagg gcttcccagg tggaagccct atttctttta
     1921 tggaatcaac aggctggaac cttgcgtcgc ccggctttcc agaatctcat gcgcacgctg
     1981 cgcatccttc agcggatatt tctgctgctc ggcgacatcg accttaatca caccgctggc
     2041 aatcaaagag aacagttcat tactggcctc ggttaattcc tcccgcgtgg tgatatagcc
     2101 ttgcagggaa gggcgtgtca catacaacga gcctttttga ttgagaatgc ctaagttcac
     2161 accggtaacc gcacctgatg agttgccaaa actgaccatt aagccgcggc gttgcaggca
     2221 atccagcgac cgttcccagg tgtctctgcc cacggaatcg tacactacgc gcactttctt
     2281 accgccggtg atctctttca atcgctcgac cagattctct tcacgatagt taataacctg
     2341 ccacgcgccg gcttttagcg cgctctgcgc tttttgcgcg gttcctacgg tgccgataag
     2401 tttcgcgccc agggcttttg cccactggca ggcaattaag ccaacgccgc cagccgctgc
     2461 gtggaacagg aactgttcat cgggtttaat ttcataggtt ttgcgcagca gataataaac
     2521 cgttaagcct ttcaggaaag atgctgcagc ttgttcaaaa gaaattgccg caggcagaat
     2581 cgccgcttta tccgcattaa tgttatgcac agagctgtaa gcgcctaacg ccgactgcgc
     2641 atagactaca cgatcgcctg ccttaatatg ctttacacca ctgccgactt tactcacgat
     2701 gcctgccgct tcggtaccta atccgctggg tagtgatggc ggcgggtaaa ggccgctgcg
     2761 gatatacgtg tcgataaaat tgatgccgat ggctttattt tcgacctgga tttcattctc
     2821 tgccggatcg gcaggagtga actctacggc ttgaagtact tccgggccac cgtgcttgtg
     2881 aaattcaatt cgtgttgcca tgtgtcctcc agaacgtatc gtcagggtct gcttcatatg
     2941 gtaaagtttc gacccattct ttatctcggt aactccattc actatggcag gaaataaacc
     3001 cttcaacaaa cagcaggctg aaccccgcga acgcgatcca caagttgccg ggctgaaagt
     3061 gcctccgcac tcgatcgaag cggagcagtc ggtgttgggc ggtttaatgc tggataacga
     3121 acgctgggac gatgtagccg agcgtgtggt ggcagacgat ttttacaccc gcccacaccg
     3181 ccatatcttt actgaaatgg cgcgtttgca ggaaagcggt agtcctatcg atctgattac
     3241 ccttgcggaa tcgctggaac gccaggggca actcgatagc gtcggtggtt ttgcttatct
     3301 ggcagagctg tcaaaaaata cgccaagtgc ggcgaacatc agtgcttatg ctgacatcgt
     3361 gcgtgaacgt gccgttgtcc gtgagatgat ctcggttgcg aatgagattg ctgaagccgg
     3421 ttttgatccg caggggcgta ccagcgaaga tctgctggac cttgctgaat cccgcgtctt
     3481 taaaattgcc gaaagtcgtg caaacaaaga cgaagggccg aagaacatcg ccgatgtgct
     3541 cgacgcaacc gtggcgcgta ttgagcagtt gtttcagcag ccacacgatg gcgttaccgg
     3601 agtaaacacc ggttatgacg atctcaacaa aaaaaccgct ggcttgcagc cgtcggattt
     3661 gatcatcgtc gccgcgcgtc cgtcgatggg taaaacaaca tttgcgatga acctcgtcga
     3721 aaacgcggcg atgttgcagg ataaaccagt acttatcttc tcgctggaga tgccttcaga
     3781 acagattatg atgcgttctc tggcgtcgct gtcgcgcgtt gaccagacta aaatccgtac
     3841 cgggcagctc gatgatgaag actgggcgcg catttccggc accatgggta ttttgctcga
     3901 aaaacgcaat atctatatcg atgactcctc cggcctgacg ccaacggaag tgcgttcccg
     3961 cgcacgccgt attgcccgtg aacacggcgg catcgggctt atcatgatcg actacctgca
     4021 actgatgcgc gtaccggcgc tttccgataa ccgtacgctg gaaattgcag aaatctcccg
     4081 ctcgctgaaa gcactggcga aagaactgaa cgtgccggtg gtggcgctgt cccagttgaa
     4141 ccgttctctg gaacaacgtg ccgacaaacg cccggtcaac tccgacctgc gtgaatctgg
     4201 ctctatcgag caggatgcgg acttgatcat gtttatctat cgtgatgagg tgtatcacga
     4261 aaacagtgat ttaaaaggca tcgcggaaat tattatcggt aaacaacgta acggcccaat
     4321 cgggacggta cgcctgacct ttaacggtca atggtcgcgc ttcgacaact atgcggggcc
     4381 gcagtacgac gacgaataat aattatttta tgaattaggt aattaaagca aacacttatc
     4441 aaggaacaca aatgcaagcg gcaactgttg tgattaaccg ccgcgctctg cgacacaacc
     4501 tgcaacgtct tcgtgaactg gcccctgcca gtaaaatggt tgcggtggtg aaagcgaacg
     4561 cttatggtca cggtcttctt gagaccgcgc gaacgctccc cgatgctgac gcctttggcg
     4621 tagcccgtct cgaagaagct ctgcgactgc gtgcgggggg aatcaccaaa cctgtactgt
     4681 tactcgaagg cttttttgat gccagagatc tgccgacgat ttctgcgcaa cattttcata
     4741 ccgccgtgca taacgaagaa cagctggctg cgctggaaga ggctagcctg gacgagccgg
     4801 ttaccgtctg gatgaaactc gataccggta tgcaccgtct gggcgtaagg ccggaacagg
     4861 ctgaggcgtt ttatcatcgc ctgacccagt gcaaaaacgt tcgtcagccg gtgaatatcg
     4921 tcagccattt tgcgcgcgcg gatgaaccaa aatgtggcgc aaccgagaaa caactcgcta
     4981 tctttaatac cttttgcgaa ggcaaacctg gtcaacgttc cattgccgca tcgggtggca
     5041 ttctgctgtg gccacagtcg cattttgact gggtgcgccc gggcatcatt ctttatggcg
     5101 tctcgccgct ggaagatcgc tccaccggtg ccgattttgg ctgtcagcca gtgatgtcac
     5161 taacctccag cctgattgcc gtgcgtgagc ataaagccgg agaacctgtt ggttatggtg
     5221 gaacctgggt aagcgaacgt gatacccgtc ttggcgtagt cgcgatgggc tatggcgatg
     5281 gttatccgcg cgccgcgccg tccggtacgc cagtgctggt gaacggtcgc gaagtaccga
     5341 ttgtcgggcg cgtggcgatg gatatgatct gcgtagactt aggtccacaa gcgcaggaca
     5401 aagccgggga tccggtcatt ttatggggcg aaggtttgcc cgtagaacgt atcgctgaaa
     5461 tgacgaaagt aagcgcttac gaacttatta cgcgcctgac ttcaagggtc gcgatgaaat
     5521 acgtggatta atcgttctgt aatatttgat tgtctgtgcc ggatgcggcg tgaatgcctt
     5581 atccggccaa taaaatccta aaaattcaat aagttgatgt tctttcatgc tcttataaag
     5641 gtcgtgcctc tggcggatgt acgtttgtca tgagtctcac tctgttgcta attgccgttc
     5701 gctcctgaac atccactcga tcttcgcctt cttccggttt attgtgtttt aaccacctgc
     5761 ccgtaaacct ggagaaccat cgcgtgtttc aaaaagttga cgcctacgct ggcgacccga
     5821 ttcttacgct tatggagcgt tttaaagaag accctcgcag cgacaaagtg aatttaagta
     5881 tcggtctgta ctacaacgaa gacggaatta ttccacaact gcaagccgtg gcggaggcgg
     5941 aagcgcgcct gaatgcgcag cctcatggcg cttcgcttta tttaccgatg gaagggctta
     6001 acagctatcg ccatgccatt gcgccgctgc tatttggtgc ggaccatcca gtactgcaac
     6061 aacagcgcgt agcaaccatt caaacccttg gcggctcagg ggcattgaaa gtgggcgcgg
     6121 atttcctgaa acgctacttc ccggaatcag gcgtctgggt cagcgatcct acctgggaaa
     6181 accacgtagc aatattcgcc ggggctggat tcgaagtgag tacttacccc tggtatgacg
     6241 aagcgactaa cggcgtgcgc tttaatgacc tgttggcgac gctgaaaaca ttacctgccc
     6301 gcagtattgt gttgctgcat ccatgttgcc acaacccaac gggtgccgat ctcactaatg
     6361 atcagtggga tgcggtgatt gaaattctca aagcccgcga gcttattcca ttcctcgata
     6421 ttgcctatca aggatttggt gccggtatgg aagaggatgc ctacgctatt cgcgccattg
     6481 ccagcgctgg attacccgct ctggtgagca attcgttctc gaaaattttc tccctttacg
     6541 gcgagcgcgt cggcggactt tctgttctgt gtgaagatgc cgaagccgct ggccgcgtac
     6601 tggggcaatt gaaagcaaca gttcgccgca actactccag cccgccgaat tttggtgcgc
     6661 aggtggtggc tgcagtgctg aatgacgagg cattgaaagc cagctggctg gcggaagtag
     6721 aagagatgcg tactcgcatt ctggcaatgc gtcaggaatt ggtgaaggta ttaagcacag
     6781 agatgccaga acgcaatttc gattatctgc ttaatcagcg cggcatgttc agttataccg
     6841 gtttaagtgc cgctcaggtt gaccgactac gtgaagaatt tggtgtctat ctcatcgcca
     6901 gcggtcgcat gtgtgtcgcc gggttaaata cggcaaatgt acaacgtgtg gcaaaggcgt
     6961 ttgctgcggt gatgtaatgc aggaaagcag gctggagcta cccagcctgc agtgaaatta
     7021 aactgtcgtc gctttcactc tttctttata gatgattttt ttgatgccat cgttctacgt
     7081 gagagataat aaacgttatt agttctttta ttgttaagct tatcctaatt atctggaatt
     7141 ccttatcctg tt
//
LOCUS       DAPHDW010000187         7142 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.190, whole genome shotgun sequence.
ACCESSION   DAPHDW010000187 DAPHDW010000000
VERSION     DAPHDW010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 7142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.190"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            91..1425
                     /gene="adeQ"
                     /locus_tag="RH057_004093"
                     /gene_synonym="yicO"
     CDS             91..1425
                     /gene="adeQ"
                     /locus_tag="RH057_004093"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HDV3130380.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAVGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDGNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            1478..1930
                     /locus_tag="RH057_004094"
     CDS             1478..1930
                     /locus_tag="RH057_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HDV3130381.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDGSEQNLLQWHRILQKAR
                     LAAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            2141..3331
                     /gene="nepI"
                     /locus_tag="RH057_004095"
     CDS             2141..3331
                     /gene="nepI"
                     /locus_tag="RH057_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HDV3130382.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(3372..3665)
                     /locus_tag="RH057_004096"
     CDS             complement(3372..3665)
                     /locus_tag="RH057_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130383.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPQASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            3887..4705
                     /gene="nlpA"
                     /locus_tag="RH057_004097"
     CDS             3887..4705
                     /gene="nlpA"
                     /locus_tag="RH057_004097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpA"
                     /protein_id="HDV3130384.1"
                     /translation="MKLTTHHLRAGAALLLAGILLAGCDQSGSDTKHIKVGVINGAEQ
                     DVAEVAKKVAKEKYGLDVELVGFSGSLLPNDATNHGELDANVFQHRPFLEQDNQAHGY
                     KLVAVGNTFVFPMAGYSKKIKSVAQIKEGATVAIPNDPTNLGRALLLLQKEKLITLKE
                     GKGLLPTALDITDNPRHLQIMELEGAQLPRVLDDPKVDVAIISTTYIQQTGLSPVHDS
                     VFIEDKNSPYVNILVAREDNKNAENVKEFLQSYQSPEVAKAAETIFNGGAVPGW"
     gene            complement(4709..5632)
                     /locus_tag="RH057_004098"
     CDS             complement(4709..5632)
                     /locus_tag="RH057_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HDV3130385.1"
                     /translation="MGSTRKGMLNVLIAAVLWGSSGVCAQYIMEQSQMSSQFLTMTRL
                     IFAGLILLTLSFVHGDKIFSIINNHKDAISLLIFSVVGALTVQLTFLLTIEKSNAATA
                     TVLQFLSPTIIVAWFSLVRKSRPGILVFCAILTSLVGTFLLVTHGNPTSLSISPAALF
                     WGIASAFAAAFYTTYPSTLIARYGTLPVVGWSMLIGGLILLPFYARQGTNFVVNGSLI
                     LAFFYLVVIGTSLTFSLYLKGAQLIGGPKASILSCAEPLSSALLSLLLLGITFTLPDW
                     LGTLLILSSVILISMDSRRRARKINRPARHE"
     gene            complement(5743..6927)
                     /locus_tag="RH057_004099"
     CDS             complement(5743..6927)
                     /locus_tag="RH057_004099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HDV3130386.1"
                     /translation="MQKTATTPSKILDLTAAAFLLVAFLTGIAGALQTPTLSIFLADE
                     LKARPIMVGFFFTGSAIMGILVSQFLARHSDKQGDRKLLILLCCLFGVLACTLFAWNR
                     NYFILLSTGVLLSSFASTANPQMFALAREHADRTGRETVMFSTFLRAQISLAWVIGPP
                     LAYELAMGFSFKVMYLTAAIAFVVCGLIVWLFLPSIQRNIPVVTQPVEILPSTHRKRD
                     TRLLFVVCSMMWAANNLYMINMPLFIIDELHLTDKLAGEMIGIAAGLEIPMMLIAGYY
                     MKRIGKRLLMLIAIVSGMCFYASVLMATTPAVELELQILNAIFLGILCGIGMLYFQDL
                     MPEKIGSATTLYANTSRVGWIIAGSVDGIMVEIWSYHALFCLAIGMLGIAMICLLFIK
                     DI"
BASE COUNT         1794 a         1598 c         1803 g         1947 t
ORIGIN      
        1 tgcaattgaa atgtgataat tatcactgaa ccgttgttgt catttgtttt acaaaagcaa
       61 gggattaaat ctcctccaat ggacaaaaaa atgaataatg acaataccga ttatgtgagt
      121 aatgaatcag ggacgctttc gcgattattt aaactacctc agcatgggac caccgtccgc
      181 acagaattga ttgcggggat gaccactttt ttaaccatgg tgtacatcgt ttttgtgaac
      241 ccgcaaatcc tcggcgcggc acaaatggac ccgaaagtgg tgtttgttac cacctgtttg
      301 attgccggta tcggcagtat tgcgatgggg atatttgcta acttacccgt ggcgctggct
      361 ccggcaatgg ggctgaacgc cttctttgcc ttcgtggtcg tgggggcgat gggcatctcc
      421 tggcagaccg ggatgggcgc aatattctgg ggcgcagttg gactattttt gctcacgctg
      481 tttcgtatcc ggtactggat gatctccaac attcccttaa gtttacgtat tggtatcacc
      541 agcggaattg gattatttat cgccttaatg ggattaaaaa atactggcgt tattgtcgcc
      601 aataaagaca cgctggtgat gattggcgat ttaagttctc acggcgtgtt gttaggtatt
      661 ttagggtttt ttattataac cgtgttgtca tcacgtcatt ttcatgccgc ggtgctggtt
      721 tctattgtgg tgacgtcttg ctgtggatta tttttcggtg atgttcattt tagcggcgtc
      781 tattccattc cgcctgatat tagcggcgtc attggtgaag tagatttgag cggcgcgtta
      841 acacttgaac tcgccggtat cattttctcc tttatgctga tcaacctatt tgattcatca
      901 ggaacattaa ttggtgtaac tgataaagcg ggcttaatag atggtaacgg taaattcccc
      961 aatatgaata aggcgctgta tgttgatagc gtcagttcgg tggcgggtgc gtttatcggc
     1021 acctcgtctg ttactgccta tattgaaagt acttctggtg tggcagtcgg tggccgcacg
     1081 gggctgactg cggttgtggt tggcgttatg ttcctgttgg ttatgttctt ctcaccgctg
     1141 gtggcgatgg ttcctcctta cgcaaccgcc ggagcgttaa tctttgttgg cgtgctgatg
     1201 acttcgagcc tggcgcgcgt taactgggat gattttaccg aatcggtgcc tgcgtttatt
     1261 accacggtga tgatgccctt tactttctcg atcaccgaag ggattgcact cggctttatg
     1321 tcgtactgca tcatgaaagt gtgcaccggg cgctggcgcg atctgaacct gtgtgtggtg
     1381 gtggtcgcag ctctgtttgc actgaagatt attctggtgg attagtaact ggcgagggtt
     1441 tccagatatc atgagttctg attacgcagg agaactcatg atctggataa tgctcgccac
     1501 gctggcggta gtgtttgtgg ttggttttcg ggtgctgaca tccggggcca gaaaagcgat
     1561 tcgccgtctc agcgatcggc tgaatatcga tgtcgtaccc gtggagtcga tggtcgatca
     1621 aatgggaaag tcagccggtg acgaattttt acgttatttg catcgcccgg atgagtcgca
     1681 cctgcaaaac gccgcgcagg tgttgctcat ctggcaaatt gtcattgtcg atggtagcga
     1741 acagaacctg ctgcaatggc atcggatttt acaaaaagcc cgtcttgccg cgccgattac
     1801 cgatgctcag gtcaggctgg cgctgggttt tctgcgcgaa accgaacctg aaatgcagga
     1861 tattaatgct tttcagatgc gctataacgc gttctttcag cctgccgagg gcgttcactg
     1921 gctgcattaa aaccgcttcg tcttatcagc ctacagaccg cgcctgacct gtcgggtgga
     1981 taagacgttc acgccgcatc cggtaatctt tgccctgttc cgtttcgatc ttaactccag
     2041 ctcaagcaac accgtaaaag ttgcaaaaac gttaaatgcg tcacacattt caatgttaaa
     2101 gtggtcggct tttcccctga aacatgccac gggtaacacc atgagtgaat ttattgccga
     2161 aaaccgcggc gcggatgcca tcacccgacc gaactggtca gccgttttct cggtggcgtt
     2221 ttgcgtcgcc tgtctgatta tcgttgagtt tttgcccgtc agtttgttga cgcctatggc
     2281 ccaggattta ggcatttcgg aaggggttgc cgggcaatcg gtgaccgtga ccgcctttgt
     2341 ggcaatgttt gccagtttgt ttattaccca gacaattcag gccactgacc gccgctacgt
     2401 tgttattttg tttgccgttt tgctgacgct ctcctgtttg ctggtttcct ttgccaactc
     2461 attcagtttg cttttaatcg gtcgtgcctg tctggggctg gcgctgggcg ggttctgggc
     2521 gatgtcggcg tcgctgacca tgcgtctggt accgccgcgt acggtgccga aggcgctgtc
     2581 ggtgatcttc ggcgcggttt ctattgcgct ggtgattgcc gcgccgttgg gcagtttttt
     2641 aggcgagctt atcggttggc gcaatgtctt taatgcggcg gcggtgatgg gcgtgttgtg
     2701 tattttctgg attatcaaat cattgccttc actgccaggc gaaccctcgc atcagaaaca
     2761 aaatactttc cgcttattac aacgtccggg tgtgatggca gggatgatcg ccatcttcat
     2821 gtctttcgcc gggcagtttg ctttcttcac gtatattcgc ccggtgtata tgaacctggc
     2881 gggattcggc gtggatggct taacgctggt gctgttgagt tttggtatcg ccagctttat
     2941 tggtacgtcg ctttcgtcgt tcattcttaa acgttcggta aaactggcct tagcaggcgc
     3001 gccgttaata ctggctgtga gtgcgttggt actgacgttg tggggaagcg ataaaatcgt
     3061 tgctaccggc gtggcgatta tctgggggct aacttttgca ttggttcccg tcggctggtc
     3121 aacgtggatc acccgctcgc tggccgatca ggcagaaaaa gccgggtcta ttcaggtggc
     3181 ggttattcag cttgctaata cctgtggcgc ggcaatcggc ggttatgcgc tggataatat
     3241 tggtctgact tcgccgttga tgttgtccgg cacattgatg ttgctgactg cattgttggt
     3301 tactgcaaag gtgaaaatga agaaatcctg atatcaggta aagaagcgtt ggtcagtagt
     3361 tgttgctgtt cttacatatc cgggcattct accttaccca acgcttccca ataattgctc
     3421 tgattattac gttccatcgc cacgatggct tcgcgtatat gttgtttatt gttttcatct
     3481 gacaacatta acttttccca cgcttcatct gataatgacg cctgtggcgt gcaaatttta
     3541 cgtaaatctt gtaataccgt ggttttctct tttgcggact gcagtgagct aaaaggcgac
     3601 tcggcgtata cgatgcctgg catagcgaaa aaagtgaaaa taagcagtag tgtcgttggt
     3661 ttcatggatt caacctttta caaatgtgta tcgaatgaag cataagtgta gtttgctttc
     3721 atcgctctca tcatgctgta cgaaacgcgc ttgcgtggtc agtaagaagt gccagacttt
     3781 atattccact tttattcctt tttattctta tcgatagcgt ttcgtttttt aaaccgcagc
     3841 gaccttaccg ctatagtcag gtaatcatta ataaaaggat aaaaaagtga aactgacaac
     3901 acatcatcta cgggcggggg ccgcattatt gctggcagga attctgctgg caggttgcga
     3961 ccagagtggc agcgatacaa aacacattaa agttggcgtt ataaatggcg cagaacaaga
     4021 tgtcgcggaa gtcgctaaaa aggtagcaaa agagaaatat ggtctcgatg ttgaactggt
     4081 aggatttagc ggttcgttgc taccgaacga tgcgacaaat catggcgaac ttgatgcgaa
     4141 tgtctttcag catcgaccct ttctcgaaca agataatcag gcgcatggtt ataaactggt
     4201 agcggtggga aatacgtttg tcttccccat ggcgggttat tccaaaaaaa tcaaaagtgt
     4261 ggcgcaaata aaagaaggcg cgacagtggc gatccccaac gacccgacca accttggtcg
     4321 tgcgctttta ctgttgcaaa aagagaagct aattactctg aaagagggaa aagggttatt
     4381 acctaccgcg ctggatataa ccgataatcc tcgtcatttg cagattatgg aactcgaggg
     4441 ggcgcagctc ccgcgcgtac tggatgatcc caaagttgat gtagcgatta tcagcaccac
     4501 ttacattcag cagacagggc tttctccggt gcacgacagc gtatttattg aagataagaa
     4561 ttcgccgtat gtgaatattt tggtggcacg ggaagataat aagaatgcag aaaacgtgaa
     4621 ggaatttctg caatcttatc aatcacccga agtcgctaaa gcggcagaaa ccatttttaa
     4681 cggtggcgcg gtgcctggct ggtaattctc actcatgccg cgccggacga tttatttttc
     4741 tggcgcggcg acgggaatcc attgaaatca aaatcaccga tgacagaatc agcagcgttc
     4801 ccagccagtc cggtaatgtg aacgtgatcc ccagcaacag caaagagagt agcgcgctac
     4861 ttaatggttc tgcacagctc aaaatgctgg cttttggacc gccaattaat tgtgctcctt
     4921 tcaggtacag actaaatgtc agggacgtac caatgaccac caaataaaaa aacgccagaa
     4981 tcaaactgcc gttaacgaca aagtttgttc cttgtctggc ataaaaaggc aacagaatca
     5041 gaccgccaat cagcatactc cagccgacga ctggtaacgt gccatagcgg gcaattagcg
     5101 ttgagggata ggtggtatag aatgcagcag caaatgccga ggcaatgccc cagaacaacg
     5161 cggcaggaga gatcgataat gacgtcggat taccgtgtgt caccaataaa aaagtcccga
     5221 ccagcgatgt caaaatagcg cagaaaacca gaatgcccgg gcgcgattta cgcaccagtg
     5281 agaaccaggc gacgataatc gtcggtgaga ggaattgcag caccgttgcc gtggctgcgt
     5341 tcgatttttc gatggttagc aaaaaagtga gctgtacagt tagcgcgcca accacggaaa
     5401 aaatcagcag gctaatggca tctttatgat tgttaataat agaaaagatt ttatcgccat
     5461 gaacaaatga cagcgtcagt agaatcaaac cggcgaatat caaacgcgtc atagtcaaaa
     5521 actgcgacga catctggctt tgctccatga tgtattgcgc gcagacccct gaacttcccc
     5581 acaacacggc ggcaatcaga acgttcagca tcccctttct ggtggaaccc atttttccct
     5641 ctgcatactg ttttgtcgtt tatgtatgca ggcatgatag caaaaatggc gaggatggct
     5701 ctgtttgcgc cacgccgatt tttcttagcc cagactggtg agctaaatat ctttaataaa
     5761 cagcaggcaa atcatcgcaa tacccaacat ccctatcgcc agacagaaca acgcatggta
     5821 gctccagatt tcaaccataa ttccgtcaac agagccggcg ataatccagc cgacgcgtga
     5881 agtatttgca tataacgttg tcgcagagcc tattttttca ggcatcaagt cctgaaaata
     5941 aagcatgccg ataccacaga gaataccaag gaagatggca ttaagaattt gcaattccag
     6001 ctcaaccgcc ggagtcgtcg ccatgagtac gctggcgtaa aaacacattc cactcacgat
     6061 agcaatgagc attaatagtc gcttgccaat acgtttcatg taatagcctg cgattaacat
     6121 catcggaatt tccagaccgg cagcgatacc aatcatttct ccagccagtt tatcggttag
     6181 atgcagttca tcaataataa atagcggcat atttatcatg tagagattat tcgccgccca
     6241 catcattgaa cagaccacaa aaagtagccg cgtatcccgc ttcctgtggg tggagggtaa
     6301 aatttctacg ggttgggtaa cgacaggaat atttctttgt attgatggca aaaacaacca
     6361 gactatcagt ccgcaaacaa caaatgcgat ggctgcggtg agatacatca ctttaaaact
     6421 aaatcccatt gccagttcat aagcgagcgg tggcccgata acccaggcaa gcgagatctg
     6481 agcacgtaaa aatgtactga acatgaccgt ctcacggcct gttctgtcgg cgtgttcacg
     6541 ggcgagggcg aacatttgcg ggtttgcggt ggaagcaaaa ctactcagaa gtacgcccgt
     6601 tgagaggaga atgaagtagt tgcgattcca cgcaaaaagc gtgcaggcca gcactccaaa
     6661 taagcaacat agcagaatca gtaatttacg gtcgccttgt ttatcggagt gccttgccag
     6721 aaattgactg accagaattc ccataatagc gctaccggtg aagaaaaaac ctaccattat
     6781 aggacgggct ttcagttcat ctgcgaggaa tatacttagg gtaggagtct gaagagcgcc
     6841 cgcaataccc gtcagaaagg cgacaagtaa aaatgccgcg gcagtgagat caagtatttt
     6901 tgatggagtg gtagccgttt tttgcattct tctgaatcac cgggcgaaaa aatataactg
     6961 agttacaaat aagtaccagt cgaatgagta ttgtattaag aatttttgag tatcgctgat
     7021 acaaccagaa agcaatgcta gcgcaatatt atagggccag caattgagat tattagtaaa
     7081 agcatgttaa gaattaatta ccgaatttat atattaatta tagtaaggca atgagctatt
     7141 tt
//
LOCUS       DAPHDW010000188         6991 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.191, whole genome shotgun sequence.
ACCESSION   DAPHDW010000188 DAPHDW010000000
VERSION     DAPHDW010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6991)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6991)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6991
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.191"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..2781)
                     /locus_tag="RH057_004100"
     CDS             complement(<1..2781)
                     /locus_tag="RH057_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HDV3130387.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLGNGLRYMI
                     YPHAHPKDQVNLWLQIHTGSLQEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMG
                     LRFGRDVNAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVI
                     TEEWRAHQDAKWRTSQARRPFLLANTRNLDREPIGLMDTVATVTPAQLRQFYQRWYQP
                     NNMTFIVVGDIDSKEALALIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRV
                     NGIALYYRLPMVQVNDEQSFIEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVK
                     IAPDYQSLFFRVNARDDNMQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAV
                     DQQAERDLRMLTSRLASSSLNNTPFLSPEETYQLSKRLWQQITVQSLAEKWQQLRKNQ
                     DAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPGRNLSLTVDADPQAEIS
                     SKETLAENLTSLTLSNGARVILAKSAGEEQKLQITAVSNKGDLSFPAQQKSLIALANK
                     AVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSARTNNPEPGFQLINQRIT
                     HSTINDNIWASLQNAQIQALKTLDQRPAEKFAQQMYETRYADDRTKLLQENQIVQFTA
                     ADALAADRQLFSSPADITFVIVGNVSEDKLVALITRYLGSIKHSDSPLAAGKPLTRAT
                     DNASVTVKEQNEPVAQFSQWKRYDSRTPVNLATRMALDAFNVALAKDLRVNIREQASG
                     AYSVSSRLSVDPQAKDISHLLAFTCQPERHDELLTLANEVMVKRLAKGISEQELNEYQ
                     QNVQRSLDIQQRSVQQLANTIVNSLIQYDDPAAWTEQEQLLKQMTVENVNTAVKQYLS
                     HPVNTYTGVLLPK"
     gene            complement(2838..5210)
                     /locus_tag="RH057_004101"
     CDS             complement(2838..5210)
                     /locus_tag="RH057_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HDV3130388.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMHFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRSDWLTSHIDENNKSAFNQGSSGSTYYSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGNVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIGKNVIARAN
                     YVYREAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFSLSFELAEPLHIRQVDINPQ
                     IVFSYIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFT
                     HQPSGLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSW
                     TPQFLKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            complement(5248..6933)
                     /locus_tag="RH057_004102"
     CDS             complement(5248..6933)
                     /locus_tag="RH057_004102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HDV3130389.1"
                     /translation="MITIPITFCMLIAKYLCLLKPFWLRKNNKTSVLLIIIILAMILG
                     VVKIQVWLNDWNNDFFNALSQKETDKLWQLVLWFPALLGIFVLISVNKTWLIKLLTIR
                     WREWLTDYYLNRWFADKNYYFTQIYGEHKNTDNPDQRIAEDILLLISKTLSLSFGFIQ
                     SLSMLITFTVILWQSAGTLSFTVGGTEWNIQGYMVYTVVLIVIGGTLFTHKVGKRIRP
                     LNVEKQRSEATFRTNLVQHNKQAELIALSNAESLQRQELSDNFHTIKENWHRLMNRQR
                     WLDYWQNIYSRSLSVLPYFLLLPQFISGQINLGGLMKSRQAFMLVSNNLSWFIYKYDE
                     LAELAAVIDRLYEFHQLTEQRPTNKPKNCQHAVQVANASIRTPDNKIILENLNFHVSP
                     GKWLLLKGYSGAGKTTLLKTLSHCWPWFKGDISSPADSWYVSQTPLIKSGLLKEIICK
                     ALPLPVDDKSLSEVLHQVGLGKLAARIHEHDRWGDILSSGEKQRIALARLILRRPKWI
                     FLDETTSHLEEQEAIRLLRLVREKLPTSGVIMVTHQPGVWNLADDICDISTVL"
BASE COUNT         1811 a         1600 c         1573 g         2007 t
ORIGIN      
        1 ttttggcaat aatactccgg tataagtatt taccggatga gaaagatatt gtttaacggc
       61 agtgttaaca ttctctaccg tcatttgttt caacaattgc tcctgctcag tccatgctgc
      121 aggatcgtca tattgaataa gactatttac aatagtgttc gctaattgtt gaacgctacg
      181 ctgttggata tcgaggctgc gctgaacgtt ttgctggtat tcattcagtt cttgctcact
      241 gatcccttta gccagacgct taaccatcac ttcattcgct aacgttaaca gttcatcatg
      301 tcgttctggt tgacaagtaa aagccagcaa atgactgata tctttggcct gaggatcaac
      361 cgagaggcga gaagaaacgc tgtatgctcc agatgcctgt tcacgaatat taacacgtag
      421 atcttttgcc agtgcgacgt taaaagcatc gagcgccata cgcgtcgcca gattaacagg
      481 tgtccgggaa tcataacgct tccactgtga aaactgtgcc acaggttcat tttgttcttt
      541 tacagtaacc gatgcgttgt ccgtcgcgcg agttaatggt ttacctgcgg ctaatggcga
      601 atcagagtgt ttgattgatc ctaagtaacg cgtaattaac gccacgagtt tgtcttctga
      661 gacattaccg acaatgacaa acgtgatatc cgctggagat gaaaacaact ggcgatcggc
      721 agccagcgca tctgcggcag taaactgtac aatctgattt tcttgcagta atttcgtgcg
      781 gtcatcagca tagcgcgtct catacatctg ctgggcgaat ttctccgctg gacgctggtc
      841 gagcgttttc aacgcctgaa tttgagcatt ttgtagcgat gcccaaatat tgtcgttaat
      901 cgtgctgtgg gtgattcgct ggttaatcaa ctgaaaacca ggttcagggt tattagtccg
      961 cgcgctaacg gagagcaacg tattcatgcc actgacttta ctgctcatgg ttaccgaatt
     1021 ttccgcactc cagcgtttca ggctggaaga ggagagctca ccaacgccgc ttccgcttac
     1081 tgctttattt gccagcgcga taagtgattt ttgctgcgca gggaaactta aatcgccttt
     1141 attcgatacg gcagtaattt gtagcttttg ctcttcaccc gcggattttg ccagaataac
     1201 cctggcacca ttggaaagtg ttaatgatgt cagattctca gccagcgttt ctttgctgct
     1261 aatttccgcc tgtgggtcag cgtctactgt taacgataaa tttctgcctg ggaagatgta
     1321 agccgccagc tttttgttgg cgtactcttt ttccagcgcc aggatagccg caggagacaa
     1381 tgcttttttg gcggcaagct cattgtttac catttgctcc caaaatgcgt cctggttctt
     1441 tcttaactgc tgccattttt ccgccagact ttgcacggta atttgctgcc acagacgttt
     1501 cgaaagttga tatgtctctt ccggcgacaa gaacggcgta ttatttaatg agctggatgc
     1561 caggcgactg gtcagcatac gtaaatcacg ttcagcttgc tgatcaaccg catttttcag
     1621 ccaggtgagg cgggtagatt tgacatcatc gagttcttca gcagaaaaac catgctgatc
     1681 aatggttgcc aactctgcca ttaatgcatt cgcagcatcc tgcatattat cgtctcgtgc
     1741 atttacacgg aaaaacagcg actgataatc gggtgcaatt ttaacgctgc gcgcagtgcc
     1801 gccagaaata gtcttcaact cgcccgactg tatgcgttcc tgcagacgtt gattgaacag
     1861 ctgaactaac atgctccatt cagcttgttc gataaagctt tgctcatcgt tcacttgtac
     1921 cattggcagg cgataataga gtgcgatgcc gttcacccgg ttttctttat cattaatgat
     1981 attaaagcgc aggtggtttt cggcttttgt cggccagacg cgattttcag ctgctttgtt
     2041 agccggaagc ttacttaaat tatcctttat cagcgccagc gcttctttac tgtcgatatc
     2101 gccgaccacg ataaaggtca tattatttgg ttgataccag cgttgataaa attggcgcaa
     2161 ttgtgccggt gtgaccgtgg cgacagtatc catcaggccg ataggttcac ggtctaaatt
     2221 acgggtatta gccagcagga aagggcggcg cgcctgagag gtgcgccatt tcgcatcctg
     2281 atgggcacgc cattcctcag taattacgcc acgttcagcg tctacttcga gtttttcaaa
     2341 ggttgcggca ttactccatt cactgaagat tgccatcact tgttgcagat tttgtttctg
     2401 cgtagtcggc aaactcacct gatacaccgt ttcgtcatag ctggtatagg cattaacatc
     2461 gcgaccaaaa cgcaggccca ttgactcaaa tgtttcgatg actttattac ccggccatgt
     2521 ttttgtgccg ttaaacatca tatgttctac aaaatgagcc acgccgcgct cattgtcttc
     2581 ttcctgcaat gaaccggtat gaatttgcag ccataaattt acctgatcct ttggatgagc
     2641 atgcggataa atcatatatc gcaagccatt gcccagttgc ccggtaatta acttttcatc
     2701 ctgcggtaag gcggcggcaa tcagccgccc ggggagcaac agagttgcca ctaacgtcag
     2761 taagaaacag aggtttctca taattatctc catgcgaaaa ccgggcgaat ttacccggtt
     2821 aagtaaaatc cgaactatta aaatttcatg ctgacatcaa gccagaaagt acggccactg
     2881 gcgtaggtcg ccacaccggt gttcgttgta tcaacagcgg ttttgctatc cagtacattg
     2941 agaatatcgg cactgatcgt caggttttgt tgtttcagaa attgtggcgt ccaggacaag
     3001 cgggtgtccc aggtcaggct gctatccagt ttttcgtcaa catactgctt gtaatctgaa
     3061 tattcgctga tgtattgcgc atttgtctta ccaaggataa tgcgagcttt acgcgcttct
     3121 tgccaggcca gcgtattcgc ccacactaac ccgctcggtt gatgcgtgaa atccatgttt
     3181 aaggagatct ttaatgggtt attaaaatct gccactggaa cgctatcgta agagaccaga
     3241 ttaccgttat aaaccacctg gttatcaccg gtattgctct cctcataacc attgtttaac
     3301 gacaagttgc ccttgctctt gatatagcta aagacaattt gtgggttaat atctacctgg
     3361 cggatatgca ggggttcggc cagttcaaaa ctgaggctga acgaatgcgt tttggttttg
     3421 ccatcgttgt tatattcagt aatggtggtt ttagtcgcgc tgtcggtacg actgcttttg
     3481 ctgatttgat catgcgcttc acggtaaaca tagtttgcgc gtgcaataac gttcttaccg
     3541 attttttgct gcaatcccat tgccagttca tcgttataag gcgttttcaa attctgataa
     3601 cgcgtcaggg ttttattacc tgataccgat tccgtccagc tattgcggat atcacgtaat
     3661 cccatatcaa gaatattccc gccatagtaa cggttataac cggcggtaat cattgaggtt
     3721 tgatcagcaa aaatatccca ttccgtcata aagcgcgggg agatattgtg gtttgacaga
     3781 tagttgtcat agtcgtaacg cacaccgggc attaacgaca cattccgcca gctaatgtga
     3841 tccgccatat acagcgtgta gttgtcaatt cccaggctgc ctttaccttt atggtaaatg
     3901 gtatggttag ttttctttcc tgcagcatta atcacatagg attcagactg gttatggcgt
     3961 tcagtccacg catcggaata gatgtattcc gcgccgaagt agggttgatg cgaaacatta
     4021 cccacggcga atttttgcca gtccaggcgt gttttgaagg tgtaattatc tacagcctgg
     4081 gaaatgtgtc ctaatccgcc acgggtgcaa cgccctgtaa tatcaccata tgtacatgaa
     4141 agttcggtgt accagatgtc atgatcgtga cgggtataat cactaatatg atcccaacca
     4201 acggtggtac gcagtttggc ccatgcgagc tgcgtatcca tatcccatgc cagaccgtag
     4261 gatttattac ccatttcgcg atcagactgc gggaaggtgc tggtattata atcacggctg
     4321 gagccggtat attttaaggt taaatcgtgg gtaaagcggt cgctggcaaa ccaggtaaat
     4381 ttgctcaatg cagtatcgat aacgttttta tactgtgccc gaccggcgac aatgccgtca
     4441 ttcgaaacat aatccgcgcg ggtgatatca gactggcggc gcgataaacc ggcggtaacg
     4501 ccgaagttat cagcgagttc ctgattaaac gacaaggtat aaaagttctt tttaaaatct
     4561 ggggagtaat aagtacttcc tgaagagcct tgattaaatg cgctcttgtt attctcatcg
     4621 atatgcgatg ttaaccagtc cgaacgcgta gtgcgataac ccaatttaac cttgctatca
     4681 tcagcgttga agcgtttgat ctttgcatca attaccccgc cattgaagcg accaaattca
     4741 accggcacaa aactgtcata aagcgtcaca ttgtccagta agctgacatc aagataatac
     4801 ccctgtgaca tcccgctaat attggttgca ctactggcat cggactcatt cgctgggttc
     4861 aggttattag ttgcactaat accgtcaatc aaataggcat tctggtaggg cgacgcaccg
     4921 tgaatagaga ttttctcagg gcgaatatct ccctggttca acgaggtact ttgcgttgaa
     4981 tccatgcgta ccgcagggtt ggttctcagc agatcgctga tattgccatt cccggtcggt
     5041 aaacgctcga tgctttcact ggtgtaatgc gtgttgccgt ttacgggtac agggacagga
     5101 gcatagaccg tcatctcttc aaaaaaatgc atgtacatgt ttttgtcatc gacggcctgc
     5161 gccacatcag cgccacataa aatgacgcca ggaataagaa ctcgcttcat ttatttatat
     5221 ctccgtacag cagggctttt attgttgtta taaaaccgtg ctaatgtcac aaatatcatc
     5281 agccaggttc cagacaccgg gttgatgtgt aaccataatg acgccgcttg tgggtagttt
     5341 ttcacgcact aaacgcagta agcgtatagc ctcttgttcc tcaagatgag aggtagtttc
     5401 gtcaagaaat atccatttcg gacgtcgtaa aattaatcgt gccaaggcga tacgttgttt
     5461 ttcgccgctg gaaagaatat ctccccagcg atcgtgctca tgaatacgcg cagccaattt
     5521 ccccagacca acctgatgca gtacttcgct caacgattta tcgtctacgg gcaggggaag
     5581 tgctttacaa ataatctctt tcagtaagcc gcttttgatt aacggtgttt gtgacacata
     5641 ccaactgtca gcaggagaag aaatatcacc tttaaaccac ggccagcagt gggataatgt
     5701 tttaagcagt gtggtttttc ccgcaccaga gtagcctttc agtaatagcc atttgcctgg
     5761 cgaaacatga aagttcaagt tctctaatat gatcttatta tcaggcgtac gaatactcgc
     5821 attagccact tgtaccgcat gttggcaatt tttaggctta ttcgtagggc gctgttcagt
     5881 gagttgatgg aactcataca agcgatcgat aaccgcagcc agctcagcaa gttcgtcata
     5941 tttataaata aaccagctta aattgttcga tactagcata aatgcctggc gcgatttcat
     6001 tagtccgccc agattgatct gcccgctaat aaactgcggc aataacagaa agtagggaag
     6061 aacgctaagc gagcgcgaat aaatattttg ccagtaatca agccaccgct gacgattcat
     6121 taaacgatgc cagttctctt taatggtatg aaaattgtcg ctcaactcct ggcgttgtag
     6181 agactcagca ttgcttagcg caatcagttc tgcctgctta ttatgctgca caagattagt
     6241 tcgaaatgtc gcttcgctac gttgtttttc cacattaagc ggacgaatac gcttaccaac
     6301 cttatgggta aataaagttc caccgatcac aatgagcaca acggtataga ccatataccc
     6361 ctggatattc cattctgttc caccaacagt gaatgagagc gtacccgcgc tttgccaaag
     6421 aataacggta aaggtgatca gcatgctaag tgactggatg aagccaaatg aaagactgag
     6481 cgttttgctg attagtaaga gaatgtcttc agcgatacgt tggtcagggt tatctgtatt
     6541 tttatgctcg ccgtagatct gcgtgaagta gtagttttta tctgcaaacc agcgattaag
     6601 gtagtaatct gttaaccatt cgcgccagcg aatagtcagc agtttaatta accatgtttt
     6661 gttcacggat attaatacaa agatccccag cagggcaggg aaccaaagga caagctgcca
     6721 aagcttatcg gtttctttct ggcttagcgc attgaaaaaa tcattattcc agtcgtttaa
     6781 ccagacctga attttaacca cgccaaggat catggcgagg ataataatga ttaacagcac
     6841 tgaagtttta ttattcttac gcagccaaaa gggcttcaac agacagagat actttgctat
     6901 caacatacaa aacgtaatgg gaatggttat cattagcgaa aattgattgt gccatccaat
     6961 gattatctat ggcaatgatt tactgatgaa a
//
LOCUS       DAPHDW010000189         6927 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.192, whole genome shotgun sequence.
ACCESSION   DAPHDW010000189 DAPHDW010000000
VERSION     DAPHDW010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6927
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.192"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(308..793)
                     /gene="sixA"
                     /locus_tag="RH057_004103"
     CDS             complement(308..793)
                     /gene="sixA"
                     /locus_tag="RH057_004103"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HDV3130390.1"
                     /translation="MQVFIMRHGDAALDAASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMNPCNLKMAKA
                     I"
     gene            complement(996..3140)
                     /gene="fadJ"
                     /locus_tag="RH057_004104"
     CDS             complement(996..3140)
                     /gene="fadJ"
                     /locus_tag="RH057_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HDV3130391.1"
                     /translation="MEMASVFTLNVRLDNIAIITIDVPGEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFVSAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEVAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPSSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNSAAHTIFASNTSSLPIGDIAAHATRPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            complement(3140..4450)
                     /gene="fadI"
                     /locus_tag="RH057_004105"
     CDS             complement(3140..4450)
                     /gene="fadI"
                     /locus_tag="RH057_004105"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HDV3130392.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPAVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLARVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SDLTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            complement(4630..4914)
                     /locus_tag="RH057_004106"
     CDS             complement(4630..4914)
                     /locus_tag="RH057_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HDV3130393.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKARSVESEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            5286..6626
                     /gene="fadL"
                     /locus_tag="RH057_004107"
     CDS             5286..6626
                     /gene="fadL"
                     /locus_tag="RH057_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HDV3130394.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAVYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
BASE COUNT         1744 a         1854 c         1631 g         1698 t
ORIGIN      
        1 gacaatataa tatataaaca ctatagccag aatttaatca agtttcaatt acatcctgcc
       61 tgattattca actctattat ctgtattaat aaatgacaat gagtgtcaaa cagacattat
      121 tcatttcaac atgtaacttt gggcaaaatc aagatacgcg cagaagaata atttccgaat
      181 gaatgtagaa agtgttttat ttatagataa ggagagtctt cgatagtagt gaggttgaca
      241 gatatccgct gcctgatgag caattatgcc caatgaatct acctcattgg gcataatttg
      301 gaacacatca gatagctttt gccatcttca gattacacgg gttcatctgc cagttgaatg
      361 taccgtttcc gctttcatca agcgttacgc tggcaatggc cgaggtggta aacataggcg
      421 gcgtttcgcc cggacataac tcggcaacca gatacccgac taacggtaag tgagagatca
      481 ccagtaccga agcaacacct tcattggtca acgcctgcaa ataggcactg accagaccaa
      541 catcgccgca gggcgttaac tccggcagaa cttctgcact ggaaggcagg ttcagacaat
      601 cccctacttc ttccagtgtt tgctcggctc gcaggaacgg gctcaccaga acgcgttcga
      661 tttccacttt ttgacctttc agccagttcg ccatcaggcg agattcgtca caaccattag
      721 tggtcaaagg acgaaccgaa tcactggcgg catcgagggc tgcgtcgccg tgacgcatga
      781 taaaaacttg catattgcac cgcttttgtt aaccagattc gcccgttttc tttcatctga
      841 aaccagagaa agaaaacggt ggccggcatt gtgccttatc cattcaccga atgaaacgct
      901 gttttttacc tcaatggcgt aagtatagtc aatatgcgtt tacattttga ccaacactcc
      961 gccattcagc gcggattcat atagctttga ccttcttatt gcaggtcagt tgcagttgtt
     1021 ttccaaaaac tttccccacg cgcgcccatc tcgaccaaac gctcgcaagg ggtaaaacgg
     1081 gaaccatact gcgtggcaag tcgttgcatt attgcaacca cttcgcccgc gccgagagaa
     1141 tcgatatagc ggaacggtcc accgagaaat ggcggaaaac caatgccaaa taccgcgcca
     1201 atatccccgt cacgcacgct acggataacc tgctcatcaa cacaacgtac tgcttcattc
     1261 agcatcaaca tcacacaccg ttcagcaacc tgcggtgcgg agagtcgccc ctgcccttgt
     1321 gcgccaatga gcgggtaaat ggcaggatcg acctgttttt tgcttttacg ccctttctga
     1381 ccataaagat agaaaccccg accatttttt ctgcctttgc gatcgtcgtt caaaattgaa
     1441 gaaacaacat ttgcaggcgc gctaaaacgt tctccataag cggcttccag tacaggaata
     1501 attttagtcc cggtgtcgat tcctacctca tccaaaagtt ggattggacc taccggaaaa
     1561 ccaaatttca ctagcgcagc atcaatgtgc tctacccgtt caccttcggt cagcatgcgg
     1621 atagcttcat taatgtaagg cgctaagatg cgattgacgt aaaacccggc tttgtcacgc
     1681 acgacaatgg gcgttttgcc ctgttttttc gccagtttta ccgtagtggc gatggtttgc
     1741 gctgatgtac ccgcatgagg aataatctcc accagcggca ttttttccac cggactgaag
     1801 aaatgcaggc cgataacttg ctcaggtcgc gtggcgtgag cggcgatatc accaatcggt
     1861 aaagatgacg tattcgaagc aaagatggta tgagcggcgc tattttgctc aacttccgcc
     1921 accatctgtt gtttcaattc gagattttca aacaccgctt caataatcag atcgcgatgg
     1981 gcaaagccgc gatagtccgt cgttccggag attaatgcca gctgtttgtc acgttcgctg
     2041 gctttgagat gacgacggcg aactttgccc tccagctgat cccaactgta cttcagcgca
     2101 tgatttatgc cctgcgggtt gatatcttta attctgaccg gaagccccgc tttacaagca
     2161 gtgacataag caataccgcc gcccatcaag ccaccaccta aaattcccac gctgtttaat
     2221 ggcgcaggcg gtgcatcact actgggatct ttcttcacgt ccgtactggc aaaaaagata
     2281 ctacgcagcg cctgcgattg tggcgtcatc gccagttcgc caaacgcccg agcttcggcg
     2341 tcataaccgc tgctggtgcc ctgcgctaat cccgtttcaa caacctccag gatgcgttct
     2401 gtcgccggat aattgccttg agttttgtgt tctgttttct tgccgaccat tttgaacagc
     2461 agcgcacgac ctaacggccc cgccagaata cgctcgcgta caggtagagg gcgggaagat
     2521 gggcgatcct tcttcgccag ctcaacagcg acttccagca aaatggagtg cggaacaacg
     2581 tcatccacca gccccagctt taatgcctgt ttcgcccgaa gttgttttcc ggtgaggatc
     2641 atctctaatg ctgtgctgac gcctatcaga cgcggtaaac gctgggtgcc gcctgaaccg
     2701 ggtaacaatc caagttgtac ttcaggcaaa ccgagcaccg ttttaggatc gtcagtacaa
     2761 acgcgaccgt ggcacgccag cgccaactcc agcccaccac ccaggcaagc gccatgaata
     2821 gccgcgataa ccggaatggg caaagcatga atctccgcca tcaactgttg gccctgccgc
     2881 gccagtgctt ccgcttcttg cgccgttttg cagttgccga tcatgttgat gtctgcgcca
     2941 gcaatgaagt tgtccggttt agcggagaca aacaccacgc ctcgcaactc tttgttttca
     3001 cggagttgct taataatggc gcgcacctgc gaggcaaact ccgccttcag ggtattcatt
     3061 ttctcacccg gtacgtcgat ggtgatgata gcaatgttgt ccagacgaac attaagagta
     3121 aacactgatg ccatttccat tattccgcct ccagaaccat tgccgcgcca agcccaccgg
     3181 cagcacaggc ggtaactaaa ccaaatccac cgccgcgacg gcgaagttca tgcaatgtct
     3241 gggtaatcat ccgcgcgccg gtcgccgcga agggatgccc gtaagcaatc gaaccgccaa
     3301 gcacgttaaa tttgctatcg tccacttcgc cagtggcatg tgcacgcccc agtacttcac
     3361 gagcaaaacg ttcactaccc agcaactgaa tattcgccag tgtctgagct gcaaaggctt
     3421 cgtgcatatc gatcaatgtc agatcgctca ttgtcaaacc ggcacgttcc agcgccagcg
     3481 gtgttgacca ggccggaccg agcaacatgt cctgccagac atcaatcgca gtaaatgcgt
     3541 agctgcgcag ataccccagc ggtaccagcc ctaattcttt cgcccgggat tcagtcatca
     3601 ggatcaccgc cgctgcgcca tcggtcagcg gcgtactgtt cgccgccgtt accgttccgt
     3661 gtttgcgatc aaacgccggg cgcagctttg cgtaatcggc aagcgaggaa ttaccgcgaa
     3721 tattgttgtc ttcgacaagc ggttgtttat aaggagggat aaaggcagtc atcacctctt
     3781 ctttgagttt accgtctgac cacgcctgag cggcacgctg atgcgaacgg tgcgctaatg
     3841 catcttgctg ttctcgggtg atgccgtagg ttttcgccat ttgttcggcg gtgtcgccca
     3901 tccgcaagcc agtagaatat tctgctaccg caggcggtac gggcattaag tcgcgcaaac
     3961 gcaggcgaga gaagagtttc agtcgctggc tcatggttcg agctttgttg acatcaacca
     4021 gcacgcgcgc cagtttttta ctgacgccaa ttggcaatac ggaagaggaa tctgccccac
     4081 cggcaatccc cgctcggata gttcccgcca tcaggctttc tgcgacgttt gcaaccgcct
     4141 ggaaactggt agcgcaagcg cggctgacgc tgtaagcatc ggtatgcaca ttcattccgg
     4201 taccgagaac aatttcacgc gcaatgttgg gggcttcagg catttgtacg acctgaccaa
     4261 agaccagctg ttcaatcact tcagcgggga tctcgctgcg tgccagcagt tcgcctacca
     4321 ccatcttccc taaatcaacc gcgggaatgc catgaaaagc cgtcgcctgg cgggcaaaag
     4381 gcgtacgtaa accgctaaca atggcgatac gatcgccctg gcgggtaacc agcggtaaaa
     4441 cctgacccat aacactcccc tgtaaaaaaa taaataaagt ggtctgacct gatcatagtc
     4501 ttaaccattt ttttacattt agccaagcgg agaaaaagga aagtgggagc tatgacacag
     4561 tgaagacagg agaaagaaaa gaaaacgccc ctgccagcaa ctgacagggg cgaacgtacc
     4621 cgagaggaat taacgcagac ccagctggaa gatcagcatt tcagcttcgc aggcaaaagt
     4681 gaaatcgata tccaggcgca caccgtcaga ctcttcagtg aaagtcgggg agattttgca
     4741 cggttcggat tccacgctac gggctttttc agtcagcgcc gccagcgttt gttctgcttc
     4801 tgcgcggttt gcaaacacgc ggctgtaaga cgcggtgcag tcggagttgt ccataatggt
     4861 gccaacatcc atacagcagc aaaccggggt ttcatcagca ctacatttac tcatcgttga
     4921 tttcctctgt atgtgcaccc aaggtgccag ataaacgttg tggatatttt acgcttccgg
     4981 aaagtgctgc tccagttgtt aattctgcaa aatcggataa gtgaccgaaa tcacacttaa
     5041 aaatgatcta aaacaaaatt cacccgaatc cgcgagtgcg caacctccaa attttgccag
     5101 ctggatcgcg tttcttagat catatttgaa aaaagatgga aacatacttg caacattcca
     5161 gctggtccga cctatactct cgccactggt ctgatttcta agatgtacct cagaccctac
     5221 acttcgcgct cctgttacag cacgtaacat agtttgtata aaaataaatc attgaggtta
     5281 tggtcatgag ccagaaaacc ctgtttacaa agtctgctct cgcagtcgca gtggcactta
     5341 tctccaccca ggcctggtcg gcaggctttc agttaaacga attttcttcc tctggcctgg
     5401 gccgggctta ttcaggggaa ggcgcaattg ccgatgatgc aggtaacgtt agccgtaacc
     5461 ccgcattgat taccatgttt gaccgcccga cattttctgc gggtgcggtt tatattgacc
     5521 cggatgtgaa tatcagcgga acgtctccgt ctggtcgtag cctgaaagcc gataacatcg
     5581 cgcctacggc atgggttccg aacatgcact ttgttgcacc gattaacgac caatttggtt
     5641 ggggcgcttc tattacctct aactatggtc tggcaacaga gtttaatgat acttatgcag
     5701 gcggctctgt cgggggtaca actgaccttg aaaccatgaa cctgaactta agcggtgcgt
     5761 atcgcttaaa taatgcatgg agctttggtc ttggtttcaa cgccgtctac gctcgcgcga
     5821 aaattgaacg tttcgcaggc gatctggggc agttggttgc tggtcagatt atgcaatctc
     5881 ctgccgggca aactcctcaa gggcaagcat tggcagctac cgccaacggt atcgacagta
     5941 ataccaaaat cgctcatctg aacggtaacc agtggggctt tggctggaac gccggaatcc
     6001 tgtatgaact ggataaaaat aaccgctatg cactgaccta ccgttctgaa gtgaaaattg
     6061 acttcaaagg taactacagc agcgatctta atcgtgcgtt taataactac ggtttgccaa
     6121 ttcctaccgc gacaggtggc gcaacgcaat cgggttatct gacgctgaac ctgcctgaaa
     6181 tgtgggaagt gtcaggttat aaccgtgttg atccacagtg ggcgattcac tatagcctgg
     6241 cttacaccag ctggagtcag ttccagcagc tgaaagcgac ctcaaccagt ggcgacacgc
     6301 tgttccagaa acatgaaggc tttaaagatg cttaccgcat cgcgttgggt accacttatt
     6361 actacgatga taactggacc ttccgtaccg gtatcgcctt tgatgacagc ccagttccgg
     6421 ctcagaatcg ttctatctcc attccggacc aggaccgttt ctggctgagt gcaggtacga
     6481 cttacgcatt taataaagat gcttcagtcg acgttggtgt ttcttatatg cacggtcaga
     6541 gcgtgaaaat taacgaaggc ccataccagt tcgagtctga aggtaaagcc tggctgttcg
     6601 gtactaactt taactacgcg ttctgataac gcgttcgcct ggataaagtc acctgcatag
     6661 caggtgactt taactccccc acttcaccga aagtagtgtc ctcattgctt acctcctgag
     6721 ttttgcaaac agcctgttgg cagcttgccc tttcaaatca ataagcggta tccacagaca
     6781 aacctgaagg gaaaggcata ttttcaggcg ttctgctcgt ccttctcaaa gagtttactt
     6841 ttctgcattt ccaggatact caacccccct ggctattgtg cgctcataca ctcaaattaa
     6901 agataggttc taaataaatg agcgttt
//
LOCUS       DAPHDW010000190         6923 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.193, whole genome shotgun sequence.
ACCESSION   DAPHDW010000190 DAPHDW010000000
VERSION     DAPHDW010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.193"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(126..1775)
                     /gene="pgi"
                     /locus_tag="RH057_004108"
     CDS             complement(126..1775)
                     /gene="pgi"
                     /locus_tag="RH057_004108"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HDV3130395.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHIAEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            2300..3649
                     /gene="lysC"
                     /locus_tag="RH057_004109"
     CDS             2300..3649
                     /gene="lysC"
                     /locus_tag="RH057_004109"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HDV3130396.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNDGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGASSHNLCFLVPGEDAEQVVQKLHFNLFE"
     gene            3794..4720
                     /locus_tag="RH057_004110"
     CDS             3794..4720
                     /locus_tag="RH057_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HDV3130397.1"
                     /translation="MATLMEKDALLNGASQCIAFLSNIIDNCSVSSHQDSGDALKRLV
                     SYRDYLYSTPAELVDFTQGKILLQQVRTQYQHEFNNTTHSENKASFDSIWQRLTNHEV
                     TPQQHPIGFVLGGQPGAGKSSLIELAKRETKDNIMIINGDDFRFLHPDFNYIYQNYGD
                     DFVTHTAKFSGETVERAIERAIVSKLNIVVEGTFRNAATPLQTLKKLKDAGYQTEVMI
                     KTTSAALSWESTNERYSKDKEAGNIARKVDKNHHDIVTGLLAENARKVFASNLSDKFA
                     VYSREKMIFSSQAATNDDIATLIQNEISGNTQ"
     gene            complement(4756..5547)
                     /locus_tag="RH057_004111"
     CDS             complement(4756..5547)
                     /locus_tag="RH057_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HDV3130398.1"
                     /translation="MPLNGVSAAMRERVSQQLKEIERRYGVKVLYACESGSRGWGFAS
                     PDSDYDVRFLYVHPLEWYLRVEPPRDVIELPIDDELDVSGWEWRKALGLLKGANPTLI
                     EWLDSPVVYQQDEETITALKAMVPMWFSPLRARWHYYSMAQKNFRGYLQGDEVRLKKY
                     FYVLRPLLAVRWVEAGKGVPPMRFAELLAGSELDAPLRAEIDELLERKQRAGEAEYGP
                     RRPLLHAFIRAEQARGEIPPLLPDSREGDVKELDSLMYQTVMRRA"
     gene            complement(5553..6680)
                     /locus_tag="RH057_004112"
     CDS             complement(5553..6680)
                     /locus_tag="RH057_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HDV3130399.1"
                     /translation="MIKKISVRKDQLALLSRNGDYYKVLHAGEHLLPWLNTPEVLLIT
                     LDGSEVPDVLADYLRRFQPDWVEKYCLVADLSEIEAGALYMDGILLEILPPSTRRLYW
                     RVEDDLTLVRMNTQQVQVQTEVMNAVLQPRRKGAVKGRDAILTVQVPAWHVGVLKIDG
                     ETQALLPPGLTAYWKINHLVEAEVVDTRLQVLEVSGQEILTKDKVNLRINLAANWRYS
                     DVLLAFSQLTKPIDHLYRELQFALREAVGTRTLDELLEDKQVIDDVVSEQVKSRMLPF
                     GMEIASLGVKDIVLPGDMKNILAQLVEAEKSAQANVIRRREETAATRSLLNTAKVMEN
                     NPVALRLKELETLERVAERIDNISVFGGLDQVLHGLVNIKG"
BASE COUNT         1707 a         1805 c         1660 g         1751 t
ORIGIN      
        1 atcacgatta aaaatctatt ttaggattaa cctcacggta tgatttccgt taaatcacag
       61 acaaggggat ttatctgata aaacaatgcc gacataacgt cggcattgtt attaaggttt
      121 ttaaattaac cgcgccacgc tttatagcgg ttaatcagac cattggtcga gctatcgtgg
      181 ctgctgattt ctttatcatc tttcagctct ggcagaatac ggtttgccag ttgtttaccc
      241 agctccacgc cccactggtc gaaggtgaag atgttcagga tcacgccctg agtaaagatt
      301 ttgtgctcat acagcgcaat caacgccccc aggctgaacg gagtgatttc gcgcagcagg
      361 atggagttgg tcgggcggtt accttcgaac actttgaacg gcaccacgta gtcaagcgtt
      421 gccggatctt taccctgatc gcgatattcc tgctcaacca cttcgcggga tttaccaaac
      481 gccagcgctt cggtctgggc gaagaagtta gacagcagtt tctggtggtg atcagagagc
      541 gggttatggg tgatagccgg ggcgatgaaa tcgcacggta ccattttggt tccctggtgg
      601 atcagctggt agaacgcgtg ctgaccgtta gtgcctggtt caccccagat aatcgggcca
      661 gtctggtaat ccacaacgtt accgttacgg tcaacatact taccgttgga ctccatattg
      721 ccctgctgga agtacgccgc gaaacggtgc atatactggt catacggcag aatcgcttca
      781 gtttccgcac caaagaaatt gttgtaccag atgccaatca gcgccagcag gacaggcagg
      841 tttttctcgg caggcgtggt ggagaaatgc ttgtccattg cgtgtgcgcc agaaagcagt
      901 tcaacgaagt tatcaaagcc gatggagaga acaatcgaca ggccaatcgc tgaccacaaa
      961 gagtaacggc cgccgaccca gtcccagaac tcgaacatgt tggcagtatc aataccaaac
     1021 tcgccaacgg ctttggcatt ggtggaaagc gccgcaaagt gttttgcaac gtgtttttca
     1081 tcacctgccg ctttcaggaa ccagtcacgc gcgctatggg cgttggtcat agtttcctga
     1141 gtggtgaagg ttttagatgc taccaggaac agcgtggttt ccgggtttac ttttttcagc
     1201 acttcagcga tgtgagtccc atcgacgtta gaaacaaagt gcatgttcag gtgatttttg
     1261 tacggacgca gtgcttcggt caccatgtac gggccgaggt cagaaccacc gatcccgatg
     1321 ttcactacgt cagtgattgc tttgccggta taacccttcc actcaccgga gataatcgct
     1381 tccgagaagg ttttcatctt ctccagcacc gcgttgactt ccggcattac gtctttgcca
     1441 tcaaccagaa tcggggtatt gctacggtta cgcagcgcta cgtgcagcac ggcgcggttt
     1501 tcagtgcggt tgatcttctc gccagagaac atcgacttaa tcgcgcccgc cagatcgcac
     1561 tctttcgcca gatcctgtaa tttcgccagc gtctcttcag tgatgcggtt tttggagtaa
     1621 tccaccagca tctgatcgtc gaaggttgcg gagaacttag aaaaacgatc gccgtcttta
     1681 gcaaaaagat cggcgatcgt aacgtctttc atttcatcga agtgtttctg tagtgcctgc
     1741 caggcagcgg tctgcgttgg attgatgttt ttcattagca atactcttct gattttgaga
     1801 attgtgactt tggaagattg tagcgccagt cacagaaaaa tgtgatggtt ttagtgccgt
     1861 tagcgtaatg ttgagtgtaa acccttagcg cagtgaagca tttattagct gaactactga
     1921 ccgccaggag tggatgaaaa atccgcatga ccccgtcgtt gacaaccgcc ccgctcaccc
     1981 tttatttata aatgtactac ctgcgctagc gcaggccaga agaggcgcgt tgcccaagta
     2041 acggtgttgg aggagccagt cctgtgataa cacctgaggg ggtgcatcgc cgaggtgatt
     2101 gaacggctgg ccacgttcat catcggctac aggggctgaa tcccctgggt tgtcaccaga
     2161 agcgttcgca gtcgggcgtt tcgcaagtgg tggagcactt ctgggtgaaa atagtagcga
     2221 agtatcgctc tgcgcccacc cgtcttccgc tcttcccttg tgccaaggct gaaaatggat
     2281 cccctgacac gaggtagtta tgtctgaaat tgttgtctcc aaatttggcg gtaccagcgt
     2341 agctgatttt gacgccatga accgcagcgc tgatattgtg ctttctgatg ccaacgtgcg
     2401 tttagtagtc ctctcggctt ctgctggtat cactaatctg ctggtcgctt tagcggaagg
     2461 gctggaacct ggcgagcgat tcgaaaaact cgacgctatt cgcaacatcc agtttgccat
     2521 tctggaacgt ctgcgttacc cgaacgttat ccgtgaagag attgaacgtc tgctggagaa
     2581 cattactgtt ctggcagaag cggcggcgct ggcaacgtct ccggcgctga cagatgagct
     2641 ggtcagccac ggcgagctga tgtcgaccct gctgtttgtc gaaatcctgc gcgaacgcga
     2701 tgttcaggca cagtggtttg atgtacgtaa agtgatgcgt accaacgacc gatttggtcg
     2761 tgcagagcca gatgtagccg cgctggcgga actggccgcg ctgcagctgc tcccacgcct
     2821 caatgacggc ttagtgatca cccagggatt tatcggtagc gaaaataaag gtcgtacaac
     2881 gacgcttggc cgtggaggca gcgattatac ggcagccttg ctggcggagg ctttacacgc
     2941 atctcgtgtt gatatctgga ccgacgtccc gggcatctac accaccgatc cacgcgtagt
     3001 ttccgcagca aaacgcattg atgaaatcgc gtttgccgaa gcggcagaga tggcaacttt
     3061 tggtgcaaaa gtactgcatc cggcaacgtt gctacccgca gtacgcagcg atatcccggt
     3121 ctttgtcggc tccagcaaag acccacgcgc aggtggtacg ctggtgtgca ataaaactga
     3181 aaatccgccg ctgttccgcg cgctggcgct tcgtcgcaat cagactctgc tcactttgca
     3241 cagcctgaat atgctgcatt ctcgcggctt cctcgcggaa gttttcggca tcctcgcgcg
     3301 gcataatatt tcggtagact taatcaccac gtcagaagtg agcgtggcat taacccttga
     3361 taccaccggt tcaacctcca ctggcgatac gttgctgacg caatctctgc tgatggagct
     3421 ttccgcactg tgccgggtgg aagtagaaga aggtctggcg ctggtcgcgt tgattggcaa
     3481 tgacctgtca aaagcctgcg gcgttggcaa agaggtattc ggcgtactgg aaccgttcaa
     3541 cattcgcatg atttgttacg gcgcatccag ccataacctg tgcttcctgg tgcccggcga
     3601 agatgccgag caggtggtgc agaagctgca ttttaatttg tttgagtaat atttccctac
     3661 tggcgtataa cgctcaccga ttgttatacg ccatcctcac tgaaaacata gattaaataa
     3721 tatctgaaaa gcaaaatcac tcctgtagaa cagtattata atcaccgaaa aatatcattt
     3781 aacaggagtg ttaatggcta ctttaatgga aaaagatgca cttttaaacg gagccagtca
     3841 gtgcatcgcg tttttatcaa atatcattga taattgctca gtttcgtcgc atcaggattc
     3901 aggcgatgct ctcaagcgat tagtcagcta tcgcgattat ctttattcta cccccgctga
     3961 acttgttgat tttacacaag gtaagattct ccttcagcag gtaagaactc aatatcagca
     4021 tgaattcaac aatactacgc attcagaaaa taaagcatct tttgattcta tttggcagcg
     4081 tctgacaaat catgaagtca caccgcaaca gcatcccatt ggttttgttt taggtgggca
     4141 acccggagca ggtaaatcat ctctgattga attagccaaa agagagacaa aagataatat
     4201 aatgattatt aacggtgatg attttcgatt tctacaccct gattttaatt atatctacca
     4261 aaattatggg gatgattttg ttacccatac agcgaaattt tccggcgaaa cggtcgaacg
     4321 tgcaattgag agagcgatag tcagtaaact caacattgtt gtcgaaggaa catttcgtaa
     4381 tgccgcgacg ccgttacaaa cattaaaaaa actcaaagat gcaggctacc aaacagaagt
     4441 catgataaaa acaacctcag cggcattaag ctgggaaagc accaatgaac ggtatagtaa
     4501 ggacaaagag gcgggaaata ttgcccgaaa agtggataaa aaccaccatg atatcgtcac
     4561 cggtttgctg gcagaaaatg cccgcaaggt gtttgcttcg aacctgtcag ataagtttgc
     4621 ggtgtattcg cgtgagaaga tgatatttag ttcacaagcg gcaacgaatg acgatattgc
     4681 aacacttata cagaatgaaa tcagcgggaa tacacaataa taataagagc tgatggcgtg
     4741 aaataacgcc gggctttatg cccggcgcat taccgtctgg tacattaagc tatccagttc
     4801 cttaacgtcc ccttctctgc tatccggtaa cagcggtgga atttccccac gcgcctgttc
     4861 cgcacgaata aacgcatgta acaatggacg gcgcggacca tattccgctt ctcctgcgcg
     4921 ctgtttacgt tccagtaatt cgtctatttc tgcacgtaac ggcgcatcaa gttcgcttcc
     4981 cgccagtaac tcagcaaagc gcattggcgg cacgcctttt cccgcttcga cccagcgcac
     5041 ggccagcagc ggacgcagca cgtaaaagta ttttttcagt cgaacttcat cgccctgcaa
     5101 atatccgcgg aagtttttct gcgccatcga atagtaatgc cagcgcgcac gcagcggaga
     5161 aaaccacatt gggaccattg ccttaagcgc cgttatcgtt tcttcatctt gttgatagac
     5221 caccggagaa tccagccatt ctatcagcgt cgggttcgcg cctttcaata atcccagcgc
     5281 cttacgccac tcccaaccgg aaacatctaa ctcgtcgtcg atgggcagct cgataacatc
     5341 gcgcggcggc tccacccgca gataccattc cagcgggtga acatacaaaa accgtacatc
     5401 gtagtcgctg tcgggcgagg caaaccccca gccgcggctc ccggattcac aggcatacag
     5461 cactttcacg ccgtagcgcc gttctatttc ttttaactgc tgcgataccc gttcgcgcat
     5521 cgcagcactg actccattta atggcatgtt actcaccctt tgatattaac caacccatgc
     5581 agaacctgat ccagaccgcc aaatacagag atgttatcga tccgctcagc cactctctcc
     5641 agcgtttcta gctcttttaa acgcaacgct accggattgt tctccatgac tttggcggtg
     5701 ttcagtaacg agcgcgtcgc tgccgtttct tcacgtcggc gaatcacatt cgcctgcgca
     5761 gatttctccg cctcaaccag ctgcgcaagg atatttttca tatcgccagg caggacgata
     5821 tctttcaccc ctaatgaagc aatctccata ccaaatggca acatgcgcga tttcacctgt
     5881 tcgctgacaa cgtcatcaat gacctgttta tcttccagca gttcatccag cgtacgagta
     5941 cctaccgctt cacgcagggc aaattgcaat tcccgataca aatgatcaat cggcttcgtt
     6001 aactgactaa atgccagcaa cacgtcgctg tagcgccagt tagcggcgag attaatccgc
     6061 agattgacct tatctttcgt taagatttct tgcccgctga cttccagtac ctgcaagcgg
     6121 gtgtcgacaa cttcagcctc aaccagatga tttattttcc agtatgctgt cagccccggc
     6181 ggtaacagcg cctgcgtttc accatcgatt ttcagcacgc ctacatgcca ggccggaacc
     6241 tgcaccgtga gtatcgcatc gcggccttta actgcccctt tgcgccgtgg ttgcaatacc
     6301 gcattcatca cctccgtttg tacctggact tgctgcgtat tcatccgcac cagtgtcaaa
     6361 tcatcttcta cacgccagta caaacggcgc gttgatggcg gcagaatctc cagcaaaata
     6421 ccgtccatat acaacgcccc ggcttcaatc tccgacaagt ctgccaccag gcaatatttc
     6481 tccacccaat caggctgaaa gcggcgcaaa taatccgcca gcacgtcggg aacttcgctg
     6541 ccatcgagcg taatcagcaa gacttctggc gtatttaacc agggtaaaag atgctcgcct
     6601 gcatgcagca ctttgtagta atcgccattt ctggagagta atgccagctg atcttttctt
     6661 acgcttattt tcttaatcat tatctattcc ttgcttaaca gccgtcgcgg aagcagacac
     6721 gaagcgacag gggaattccc ttttgcctgt tacccggcgt ctttatcggc gacgttatcg
     6781 gggatcgctg actgcgcaat ccctcacttt ttatttaata agttacaacc agtattcatt
     6841 tcaggaatga atgtgggaat cgaacccata attggcgtca aaggctgacg gaacacgccc
     6901 ggaaaacagc ctggccgttc ctg
//
LOCUS       DAPHDW010000191         6923 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.194, whole genome shotgun sequence.
ACCESSION   DAPHDW010000191 DAPHDW010000000
VERSION     DAPHDW010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6923
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.194"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            440..1429
                     /gene="araF"
                     /locus_tag="RH057_004113"
     CDS             440..1429
                     /gene="araF"
                     /locus_tag="RH057_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
                     /protein_id="HDV3130400.1"
                     /translation="MHKFTKALAAIGLAAVMSQSAMAENLKLGFLVKQPEEPWFQTEW
                     KFADKAGKDLGFEVIKIAVPDGEKTLNAIDSLAASGAKGFVICTPDPKLGSAIVAKAR
                     GYDMKVIAVDDQFVNAKGKPMDTVPLVMMAATKIGERQGQELYKEMQKRGWDVKESAV
                     MAITANELDTARRRTTGSMDALKAAGFPEKQIYQVPTKSNDIPGAFDAANSMLVQHPE
                     VKHWLIVGMNDSTVLGGVRATEGQGFKAADIIGIGINGVDAVSELSKAQATGFYGSLL
                     PSPDVHGYKSSEMLYNWVAKDVEPPKFTEVTDVVLITRDNFKEELEKKGLGGK"
     gene            1499..3013
                     /gene="araG"
                     /locus_tag="RH057_004114"
     CDS             1499..3013
                     /gene="araG"
                     /locus_tag="RH057_004114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HDV3130401.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLSIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIDNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKAPGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEDRKAEGIIPVHSVRDNINISARRKHVLGGCVINNGWEENNADHHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            3028..4014
                     /gene="araH"
                     /locus_tag="RH057_004115"
     CDS             3028..4014
                     /gene="araH"
                     /locus_tag="RH057_004115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HDV3130402.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGILCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            4181..4981
                     /gene="otsB"
                     /locus_tag="RH057_004116"
     CDS             4181..4981
                     /gene="otsB"
                     /locus_tag="RH057_004116"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HDV3130403.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITTALQQKRENNRSDDYESFSRSI"
     gene            4956..6380
                     /gene="otsA"
                     /locus_tag="RH057_004117"
     CDS             4956..6380
                     /gene="otsA"
                     /locus_tag="RH057_004117"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase"
                     /protein_id="HDV3130404.1"
                     /translation="MSRLVVVSNRIAPPDEHAASAGGLAVGILGALKAAGGLWFGWSG
                     ETGNEDQPLKKVKKGNITWASFNLSEQDLDEYYNQFSNAVLWPAFHYRLDLVQFQRPA
                     WDGYLRVNALLADKLLPLLQDDDIIWIHDYHLLPFAHELRKRGVNNRIGFFLHIPFPT
                     PEIFNALPTYDTLLEQLCDYDLLGFQTENDRLAFLDCLSNLTRVTTRSAKSHTAWGKA
                     FRTEVYPIGIEPKEIAKQAAGPLPPKLAQLKAELKNVQNIFSVERLDYSKGLPERFLA
                     YEALLEKYPQHHGKIRYTQIAPTSRGDVQAYQDIRHQLENEAGRINGKYGQLGWTPLY
                     YLNQHFDRKLLMKIFRYSDVGLVTPLRDGMNLVAKEYVAAQDPANPGVLVLSQFAGAA
                     NELTSALIVNPYDRDEVAAALDRALTMSLAERISRHAEMLDVIVKNDINHWQECFISD
                     LKQIVPRSAESQQRDKVATFPKLA"
     gene            complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="RH057_004118"
     CDS             complement(6387..6815)
                     /gene="uspC"
                     /locus_tag="RH057_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspC"
                     /protein_id="HDV3130405.1"
                     /translation="MSYSNILVAVAVTPESQQLLAKAVSIARPVKGHISLITLASDPE
                     MYNQLAAPMLEDLRSVMQEETQSFLDKLIQDAGYPVDKTFIAYGELSEHILEVCHKHH
                     FDLVICGNHNHSFFSRASCSAKRVIASSEVDVLLVPLTGD"
BASE COUNT         1748 a         1623 c         1805 g         1747 t
ORIGIN      
        1 taatgagcac agtgataatt aatggcgaaa tgaatgacgt gcattttcca catctttgag
       61 ttgcggttat tacaccattt caaaaactca acgccaggta atgcggccta ttgactggtt
      121 aaaaagaaga catcccgcat gggtaccaaa gacaacaagg atttccaggc taatcttatg
      181 gattaatctg ctgtgcattc gacaatttgt ctgacaaatt ggctttccct tatgtctttt
      241 cccgctaaat ttatgcacgt tctcactgta attctgcgat gtgatattgc tctcctatgg
      301 agaattaatt tctcgctaaa actatgtcaa cacggtcact tatcttttag ataaaaggta
      361 atgctttgtt ttccgattaa tttaacgaaa gtcattcgtt tttgccctac acaaaacgac
      421 actaaagctg gagagaacca tgcacaaatt tactaaagcc ctggcagcca ttggtctggc
      481 agccgttatg tcacaatccg ctatggcgga gaacctgaag ctcggttttc tggtgaagca
      541 accggaagag ccttggttcc agaccgaatg gaagtttgcc gataaagccg ggaaggattt
      601 agggtttgag gttattaaga ttgccgtgcc ggatggcgaa aaaacattga acgcgatcga
      661 cagcctggct gccagtggcg caaaaggttt cgttatttgt actccggacc ccaaactcgg
      721 ctctgccatc gtcgcgaaag cgcgtggcta cgatatgaaa gtcattgccg tggatgacca
      781 gtttgttaac gccaaaggta agccaatgga taccgttccg ctggtgatga tggcggcgac
      841 taaaattggc gaacgtcagg gccaggaact gtataaagag atgcagaaac gtggctggga
      901 tgtcaaagaa agcgcggtga tggcgattac cgccaacgaa ctggataccg cccgccgccg
      961 tactacggga tctatggatg cgctgaaagc ggccggattc ccggaaaaac aaatttatca
     1021 ggtacctacc aaatctaacg acatcccggg ggcatttgac gctgccaact caatgctggt
     1081 tcaacatccg gaagttaaac attggctgat cgtcggtatg aacgacagca ccgtgctggg
     1141 cggcgtacgc gcgacggaag gtcagggctt taaagcggcc gatatcatcg gcattggcat
     1201 taacggtgtg gatgcggtga gcgaactgtc taaagcacag gcaaccggct tctacggttc
     1261 cctgctgcca agcccggacg tacatggcta taaatccagc gaaatgcttt acaactgggt
     1321 agcaaaagac gttgaaccgc caaaatttac cgaagttacc gacgtggtac tgatcacgcg
     1381 tgacaacttt aaagaagaac tggagaaaaa aggtttaggc ggtaagtaat ttgccggaaa
     1441 aattcccctc tgcatgatgc agagggggtg tgaacgacca gtgattcacg gagacgttat
     1501 gcaacagtct accccgtatc tctcatttcg cggcatcggt aaaacgtttc ccggcgttaa
     1561 ggcgctgacg gatattagtt ttgactgcta tgccggtcag gttcatgcgt tgatgggtga
     1621 aaatggcgca ggaaaatcaa ctctcttaaa aatcctcagc ggcaactatg cgccaaccac
     1681 gggttctgta gtgattaatg ggcaggaaat gtccttttcc gacacgaccg cagcacttaa
     1741 tgcgggtgtg gcgattattt accaggaact gcatctcgtg ccggaaatga ccgtcgcgga
     1801 aaacatctat ctcggccagc tgccgcataa aggcggcatt gtgaatcgct cattgctgaa
     1861 ttatgaggcg ggtttacaac ttaaacatct tggtatggat attgacccgg acacgccgct
     1921 gaaatatctc tccattggtc agtggcagat ggttgaaatc gccaaggcgc tggcgcgtaa
     1981 cgctaaaatt atcgcctttg atgagccaac cagctccctt tctgcccgcg aaatcgacaa
     2041 tcttttccgc gttattcgtg aactgcgaaa agaggggcgg gtgatcttat acgtttctca
     2101 ccgtatggaa gaaatatttg ccctcagcga tgccatcact gtctttaaag atggacgtta
     2161 tgtcaaaacc tttaccgata tgcagcaggt tgaccacgac gcgctggtgc aggcgatggt
     2221 cgggcgcgac attggcgata tctacggctg gcaacctcgt agttatggcg aggagcgcct
     2281 gcgtcttgat gctgtgaaag caccaggcgt gcgtacgcca ataagtctgg cggttcgcag
     2341 tggtgaaatt gttgggctgt ttggtctggt aggagcgggg cgtagcgaat taatgaaagg
     2401 cttgtttggc gggacgcaaa tcaccgccgg tcaggtttat atcgaccaac agccgatcga
     2461 tattcgtaaa ccgagccacg ccattgccgc aggcatgatg ctctgcccgg aagatcgcaa
     2521 agcggaaggc attattcccg tgcactccgt tcgcgacaat atcaacatca gtgccagacg
     2581 taaacatgtg ctcggcggtt gtgtaatcaa caacggttgg gaagaaaaca atgccgatca
     2641 ccacattcgt tcgctcaaca tcaaaacgcc gggcgctgag caactgatca tgaatctctc
     2701 aggcggaaat cagcaaaaag ccattctggg ccgctggtta tcggaagaga tgaaggtcat
     2761 tttgctggat gaacctacgc gcggcattga tgttggtgct aagcatgaaa tttacaacgt
     2821 gatttatgcg ctggcggcgc agggtgtggc ggtgctgttt gcctccagcg acttacctga
     2881 agtcctcggc gttgccgacc ggattgtggt gatgcgggaa ggtgaaatcg ccggtgaatt
     2941 gttacacgag caggcagatg agcgtcaggc actgagcctt gcgatgccta aagtcagcca
     3001 ggcagttgcc tgagtaagga gagtatgatg tcttctgttt ctacatcggg gtctggcgca
     3061 cctaagtcgt cattcagctt cgggcgtatc tgggatcagt acggcatgct ggtggtgttt
     3121 gcggtgctct ttatcgcctg tgccattttt gtcccaaatt ttgccacctt cattaatatg
     3181 aaagggttgg gcctggcaat ttccatgtcg gggatggtgg cgtgtggcat gttgttctgc
     3241 cttgcttccg gtgactttga cctttctgtc gcctccgtaa ttgcctgtgc gggtgtcacc
     3301 acggcggtgg ttatcaacct gactgaaagc ctgtggattg gcgtggcagc ggggttgctg
     3361 cttggcattc tctgtggcct ggtcaatggc tttgttatcg ccaaactgaa aataaatgct
     3421 ctgatcacaa cactggcaac gatgcagatt gttcgaggtc tggcgtacat catttcagac
     3481 ggtaaagcgg tcggtatcga agatgaaagc ttctttgccc ttggttacgc taactggttc
     3541 ggtctgcctg cgccaatctg gctcaccgtc gcgtgtctga ttatctttgg tttgttgctg
     3601 aataaaacca cctttggtcg taacaccctg gcgattggcg ggaacgaaga ggctgcgcgt
     3661 ctggcgggtg taccggttgt tcgcaccaaa attattatct ttgttctctc tggcctggta
     3721 tctgcgatag ccggaattat tctggcttca cgtatgacta gtgggcagcc aatgacgtcg
     3781 attggttatg agcttattgt tatctccgcc tgcgttttag gtggcgtttc tctgaaaggt
     3841 ggcatcggaa aaatctcata tgtggtggcg ggtatcttaa ttttaggcac cgtggaaaac
     3901 gccatgaacc tgcttaatat ttctcctttc gcgcagtacg tggttcgcgg cttaatcctg
     3961 ctggcagcgg tgatcttcga ccgttacaag caaaaagcga aacgcactgt ctgatgcttt
     4021 tttccacaac aatttaacgt tttttcccac cacagccagc cgccacaacg gttggctgtt
     4081 cttcattgca aatggcgacc cccgtcacac tgtctatact tacatgtctg taaagcgcgt
     4141 tctgcgcaaa acaataagaa aagagaagga ggagagacgg gtgacagaac cgttaaccga
     4201 aacccctgaa ctatccgcga aatatgcctg gttttttgat cttgatggaa cgctggcgga
     4261 aatcaaaccg catcccgatc aggtcgtcgt gcctgacaat attctgcaag gactacagct
     4321 actggcaacc gcaagtgatg gtgcattggc attgatatca gggcgctcaa tggtggagct
     4381 tgacgcactg gcaaaacctt atcgcttccc gttagcgggc gtgcatgggg cggagcgccg
     4441 tgacatcaat ggtaaaacac atatcgttca tctgccggat gcgattgcgc gtgatattag
     4501 cgtgcaactg catacagtca tcgctcagta tcccggcgcg gagctggagg cgaaagggat
     4561 ggcttttgcg ctgcattatc gtcaggctcc gcagcatgaa gacgcattaa tgacattagc
     4621 gcaacgtatt actcagatct ggccacaaat ggcgttacag cagggaaagt gtgttgtcga
     4681 gatcaaaccg agaggtacca gtaaaggtga ggcaattgca gcttttatgc aggaagctcc
     4741 ctttatcggg cgaacgcccg tatttctggg cgatgattta accgatgaat ctggcttcgc
     4801 agtcgttaac cgactgggcg gaatgtcagt aaaaattggc acaggtgcaa ctcaggcatc
     4861 atggcgactg gcgggtgtgc cggatgtctg gagctggctt gaaatgataa ccaccgcatt
     4921 acaacaaaaa agagaaaata acaggagtga tgactatgag tcgtttagtc gtagtatcta
     4981 accggattgc accaccagac gagcacgccg ccagtgccgg tggccttgcc gttggcatac
     5041 tgggggcact gaaagccgca ggcggactgt ggtttggctg gagtggtgaa acagggaatg
     5101 aggatcagcc gctaaaaaag gtgaaaaaag gtaacattac gtgggcctct tttaacctca
     5161 gcgaacagga ccttgacgaa tactacaacc aattctccaa tgccgttctc tggcccgctt
     5221 ttcattatcg gctcgatctg gtgcaatttc agcgtcctgc ctgggacggc tatctacgcg
     5281 taaatgcgtt gctggcagat aaattactgc cgctgttgca agacgatgac attatctgga
     5341 tccacgatta tcacctgttg ccatttgcgc atgaattacg caaacgggga gtgaataatc
     5401 gcattggttt ctttctgcat attcctttcc cgacaccgga aatcttcaac gcgctgccga
     5461 catatgacac cttgcttgaa cagctttgtg attatgattt gctgggtttc cagacagaaa
     5521 acgatcgtct ggcgttcctg gattgtcttt ctaacctgac ccgcgtcacg acacgtagcg
     5581 caaaaagcca tacagcctgg ggcaaagcat ttcgaacaga agtctacccg atcggcattg
     5641 aaccgaaaga aatagccaaa caggctgccg ggccactgcc gccaaaactg gcgcaactta
     5701 aagcggaact gaaaaacgta caaaatatct tttctgtcga acggctggat tattccaaag
     5761 gtttgccaga gcgttttctc gcctatgaag cgttgctgga aaaatatccg cagcatcatg
     5821 gtaaaattcg ttatacccag attgcaccaa cgtcgcgtgg tgatgtgcaa gcctatcagg
     5881 atattcgtca tcagctcgaa aatgaagctg gacgaattaa tggtaaatac gggcaattag
     5941 gctggacgcc gctttattat ttgaatcagc attttgaccg taaattactg atgaaaatat
     6001 tccgctactc tgacgtgggc ttagtgacgc cactgcgtga cgggatgaac ctggtagcaa
     6061 aagagtatgt tgctgctcag gacccagcca acccgggcgt tcttgttctt tcgcaatttg
     6121 cgggagcggc aaacgagtta acgtcggcgt taattgttaa cccctacgat cgtgacgaag
     6181 ttgcagctgc gctggatcgt gcattgacta tgtcgctggc ggaacgtatt tcccgtcatg
     6241 cagaaatgct ggacgttatc gtgaaaaacg atattaacca ctggcaggag tgcttcatta
     6301 gcgacctaaa gcagatagtt ccgcgaagcg cggaaagcca gcagcgcgat aaagttgcta
     6361 cctttccaaa gcttgcgtag gagcggttaa tcacccgtaa gtggaactaa cagcacatcg
     6421 acctcacttg aggcaatcac tcttttcgcc gagcaggatg ctcgcgaaaa gaaactgtga
     6481 ttgtgattac cgcaaatcac caaatcgaaa tgatgtttgt gacatacttc cagaatatgt
     6541 tcgcttaact caccgtaggc aataaacgtc ttgtcaacgg gataccccgc atcctgaatt
     6601 aacttatcaa gaaagctttg cgtttcttct tgcatcacgc tgcgtaaatc ttcaagcatc
     6661 ggcgcggcta attgattgta catttccggg tcggaagcga gagtaattaa actgatgtgt
     6721 ccctttactg gcctggcgat agataccgct tttgccagca gttgctgact ttccggtgta
     6781 accgcaacag cgacaagaat attgctatag ctcataacct gttccttatt ctgtgaactt
     6841 caggtgacat taaagcttta ctcctaatgg agaaacgacg caatcccaac tcggtcaaac
     6901 aggtaaatat tagtctgatt att
//
LOCUS       DAPHDW010000192         6921 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.195, whole genome shotgun sequence.
ACCESSION   DAPHDW010000192 DAPHDW010000000
VERSION     DAPHDW010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.195"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            229..1761
                     /locus_tag="RH057_004119"
     CDS             229..1761
                     /locus_tag="RH057_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HDV3130406.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNYLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGWATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            complement(1813..2532)
                     /gene="fadR"
                     /locus_tag="RH057_004120"
     CDS             complement(1813..2532)
                     /gene="fadR"
                     /locus_tag="RH057_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HDV3130407.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            2754..4295
                     /gene="nhaB"
                     /locus_tag="RH057_004121"
     CDS             2754..4295
                     /gene="nhaB"
                     /locus_tag="RH057_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HDV3130408.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYKVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            4441..4971
                     /gene="dsbB"
                     /locus_tag="RH057_004122"
     CDS             4441..4971
                     /gene="dsbB"
                     /locus_tag="RH057_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HDV3130409.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGLEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5017..6285)
                     /gene="umuC"
                     /locus_tag="RH057_004123"
     CDS             complement(5017..6285)
                     /gene="umuC"
                     /locus_tag="RH057_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HDV3130410.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVVLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYPSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMTVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(6285..6704)
                     /locus_tag="RH057_004124"
     CDS             complement(6285..6704)
                     /locus_tag="RH057_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HDV3130411.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
BASE COUNT         1651 a         1763 c         1703 g         1804 t
ORIGIN      
        1 agctggctca catctccctg actctgcggc aatatggcgt ctttagtatc tcctgctgaa
       61 cgcccttttt atccagcata aaattccgtt cagaagcgga ttagtggcac tctgataata
      121 agaaatcgca aagaaaaaaa ttctccggcg acgcggtttt taacaggtgt tctatgcttg
      181 aaatgaggtg ctccacacag agagcgccga caacaatgag gtgcgcgtat ggcgacgatc
      241 gattctatga ataaggacac cacacgtttg agcgatggac ccgactggac gttcgacctg
      301 ctggatgttt atctggcaga gatagaccgg gtggcgaaac tctaccggct ggatacctac
      361 ccgcaccaga ttgaagtgat aacctcagaa cagatgatgg atgcctactc cagcgtcggc
      421 atgccaatta actatccgca ctggtcattc ggtaaaaagt ttatcgagac tgaacggctg
      481 tataagcacg gtcagcaagg actggcctat gaaatcgtca ttaactctaa cccgtgtatc
      541 gcttacctga tggaagagaa caccattacc atgcaggcgc tggtgatggc acacgcctgc
      601 tatgggcata actctttctt taaaaacaat tacttattcc gcagttggac cgacgccagt
      661 tcgattgtcg attatctgat ttttgcccgt aaatatatta ccgagtgcga agaacgttat
      721 ggcgttgatg aagtagaacg gcttctggac tcgtgccacg cgctgatgaa ctacggcgtg
      781 gaccggtaca aacgcccgca aaaaatctcg ctgcaagaag agaaagcccg gcagaaaagt
      841 cgcgaagagt atctacaaag tcaggtcaat atgctctggc gtaccctgcc gaagcgcgag
      901 gaagagaaaa cggttgctga agcgcgccgc tatccgtccg aaccacaaga aaacctgctc
      961 tattttatgg agaaaaatgc gccactgctg gaatcatggc agcgtgaaat cctgcgtatt
     1021 gtgcgtaagg tgagccagta tttttatccg caaaaacaga ctcaggtgat gaacgaaggc
     1081 tgggcgacct tctggcacta taccatcctt aaccatctgt atgatgaagg gaaagtaacg
     1141 gaacgtttta tgctggagtt tttgcacagc cacaccaatg tggtcttcca gcccccctat
     1201 aacagcccgt ggtacagcgg catcaacccg tatgccctcg ggttcgccat gttccaggat
     1261 attaaacgga tttgtcagtc gccaacggaa gaagacaaat actggttccc ggatatcgcc
     1321 ggttccgact ggctggaaac gctgcatttc gcgatgcgtg atttcaaaga tgagagtttt
     1381 atcagccagt ttctgtcacc gaaagtgatg cgtgatttcc gcttcttcac cgtgctggat
     1441 gatgatcggc ataattatct ggagatttcc gctattcata atgaagaagg ttatcgggag
     1501 atccgtaacc ggttatcgtc gcaatataac ttaagtaatc tggagccgaa tattcagatc
     1561 tggaacgtgg atttgcgcgg cgaccgttcg ctgacgctgc gttacattcc acataatcgc
     1621 gcaccgctgg atcgggggcg caaagaagtg ctgaagcatg tgcatcgcct gtggggattt
     1681 gatgtgatgc tcgaacagca aaacgaagac ggcagcatcg agttgctgga acgttgcccg
     1741 ccaagaatgg gaaatctgta aaaacaacaa aaaacccctc gtttgagggg tttgctcttt
     1801 aaacggaagg gattatcgcc cctgaatggc taaatcaccc ggcagatttt tctgcatccg
     1861 gtgccaaatc tcgccactct catgcccata gcgacgcact gtttcgtaca cctgatcgtg
     1921 cgcgccttca ctgcacaacg ccgacagttt gtggtagaag cccagcgcca gactgcgcgc
     1981 ttccggattg gcgaaatagt gacgaccaat gcgcgtatac agccctttca tcccgttaag
     2041 gatcagaccg taaatcgggt tgccggaagc aaacgccagg ccgcggaata tgttgtaatc
     2101 cagctcggca aaggcatcgg cgtgatcggc cacttcatta gcggtagcca gcacttcctg
     2161 cgctttatcg ggatgctgac gaaacgcggt gcgaataaaa atagtggaaa tattggtacg
     2221 caccgacagc aaattatcaa taagctgcgg cacactttcg tgatccagtc gcgccagtgt
     2281 ttcaaggata tttaaaccag aggtttccca gaaattattc accttcgtcg gcttgccatg
     2341 ttgaatggtc aaccagccat ctcgtgccag acgctgtaac acttcacgta acgtagtacg
     2401 cgttacgcca attaattctg aaagttcacg ttctgcgggc aaaatagtcc cgggagggaa
     2461 gcggttattc cagatacttt caataatgta ctcttccgcg aaacccgccg ggctttgcgc
     2521 cttaatgacc atagtgagat ttccataaca cagcaaaaca aagttggact catcatacca
     2581 gagggctaac tacgctgata gcagacaaaa agaagaaaaa agggatcaag gcttcatttt
     2641 ccgcatctct ttccccttga ctatcaagac ttgcctcact cctgcctgac cgttaagctt
     2701 tgcgtttaaa cataaaaaca tcattgatta tttgtaaggt aagggaaacc atcatggaga
     2761 tctcctgggg ccgcgcgcta tggcgcaatt ttttgggcca gtcccccgac tggtacaaac
     2821 tcgccctcat cattttctta atcgtaaacc cgttaatttt cctcatcagc cctttcgtcg
     2881 cgggctggtt gctggtagcg gaatttattt tcactctggc gatggccctg aaatgctacc
     2941 cgctgctccc cggtggtctg ttggctatcg aagcggtatt catcggcatg accagcgcgg
     3001 aacacgtccg tgaagaggtg gcggcaaatc ttgaagtctt gctgttattg atgtttatgg
     3061 tggcgggtat ctattttatg aaacagctgt tgctgttcat atttacccgt ttgctgttaa
     3121 gcattcgctc caaaatgctg ctgtcgctct ctttttgcgt ggcggcagcg ttcctctccg
     3181 cgttcctcga tgccttaacc gtcgtggcgg tggtgatcag cgttgctgtc ggtttttatg
     3241 gtatttatca ccgcgtagcc tcttcccgta ccgaagatac cgacctgcaa gacgatagtc
     3301 atatcgacaa gcattacaaa gtggttctgg aacagttccg tggctttctg cgtagcctga
     3361 tgatgcatgc cggtgtcggc actgcattag gcggcgtgat gaccatggtg ggcgaaccac
     3421 agaacctgat catcgctaaa gcggctggct ggcattttgg cgatttcttc ctgcgcatgt
     3481 cgccggtgac cgttccggtt ctgatttgtg gcctgttaac ctgcctgctg gtagagaagc
     3541 tgcgttggtt tggctacggt gaaacgctgc cggagaaagt ccgcgaagtg ttgcaacagt
     3601 ttgacgatca aagccgccac cagcgtaccc gtcaggataa aatccgtctg attgtccagg
     3661 cgattattgg cgtctggctg gtgactgcgc tggcgttgca tctggcggaa gttggcttga
     3721 ttggtttgtc agtcattatt ctggcgacat cattgaccgg tgtcaccgat gaacatgcta
     3781 tcggtaaagc cttcaccgaa tctctgccat tcaccgcact gttgacggtg tttttctcgg
     3841 tagtcgcggt gattatcgac caacaactgt tttcgccaat tattcagttt gtgttgcagg
     3901 catcggaaca tgctcagctg tcgctgttct atattttcaa cggtctgctg tcatccattt
     3961 cggataacgt cttcgtgggg acgatttata tcaacgaagc gaaagcggca atggaaagtg
     4021 gcgctatcac gttgaagcaa tacgagctgc tggcggtcgc cattaatacc ggtaccaatc
     4081 tgccctccgt cgctacgccg aacggtcagg ctgcgttcct gttcctgctg acctctgcac
     4141 tcgcgccatt gattcgcctc tcttatggcc gcatggtgtg gatggccctg ccttacaccc
     4201 tcgtactgac actcgtcggc ttgctctgcg tcgagtttac gcttgcccct gtaaccgaat
     4261 ggtttatgca aatgggctgg atagcaacgc tttgataaca acttaccggg catataaatg
     4321 cccggtttgc cttttcgccc gataattgtc caattgcttc ctatttttgc tgcctcctgg
     4381 tggcgcagcg aatgaattgg tttaaactgc gcactctatg catattgcag ggaaatgatt
     4441 atgttgcgat ttttgaacca atgttcacaa ggccggggcg cgtggctgtt gatggcgttt
     4501 actgctctgg cactggaact gacggcgctg tggttccagc atgtgatgtt actgaaacct
     4561 tgcgtgctct gtatttatga acgttgtgcg ttattcggcg ttctgggtgc tgcgctgatt
     4621 ggcgcgatcg ccccgaaaac tccgctgcgt tatgtagcga tggttatctg gttgtatagt
     4681 gcgttccgcg gtgtgcagtt aacttacgag cacaccatgc ttcagctcta tccttcgccg
     4741 tttgccacct gtgattttat ggttcgtttc ccggaatggc tgccgctgga taaatgggtg
     4801 ccgcaagtgt ttgtcgcctc tggcgattgt gccgagcgtc agtgggattt tttaggtctg
     4861 gaaatgccgc agtggctgct cggtattttt atcgcttacc tgattgtcgc ggtgctggtg
     4921 gtgatttccc agccgtttaa agcgaaaaaa cgtgacctgt tcggtcgcta atcccttcag
     4981 cgctcctgcg ggagcgcttt tttcctgccg ctatatttat ttgacccgca gtaaatcaga
     5041 acttcgcgtt gtataacgtg gtgaaagcat ggcacgcttc atctgccatt gttgctggat
     5101 cccctgcccg gcaaaataga gtgttcctct gccctcttta gcattcagtg tatccattac
     5161 cgtcatcaat tgctcactcc cggggcgcgg tgcgttgtca tcgaataaat tgagctgcgc
     5221 gactccctga ctgaagaaat cccccagcat cacgcccgct ttttggtagc gatggcccgc
     5281 ttgccagatg gcatccagag atcgcgtagc agcgttaatg atatccctgc tgtcctgagt
     5341 gggcgtcagc agttttaccg acgcgctatt gccgtaataa ggttcattga gcgcaaatgg
     5401 tgacgtctta ataaatgtgg agataaaccg acaatattga tgctcgctgc gaagtttttc
     5461 cgccgcccgg gcagcgtaac tacaaatggc ctgccgcatc gatggataat ccgtgatgcg
     5521 ttcaccaaac gagcgggaac agataatttc ctgcttcgtc ggtgcaaact cttccagttg
     5581 caaacagggt tcgccgcgca gttcacgcac cgttctttcg agcacgacat taaaatgttt
     5641 acggataaac cggatatctg tatccgccaa atcgagaacg gttttgatcc ccatcgcgtc
     5701 cagttttttg ctgatccgcc gtccaatccc ccagacgtca tccacgggga gagcagacat
     5761 taatttacgc tggcgttcca gatttgataa atccaccacc ccccccgtct gccgctgcca
     5821 tttttttgcc gcatgattgg caagcttagc caacgttttg gtctgggcga tccccacacc
     5881 aacagtaaga tgggtacgtt gtagcaccgt tgcgcgaatt tctctgccaa aatcagtcag
     5941 atcgcgacaa ttacgcacac ctgtcagatc gcagaatgcc tcatcaatac tgtaaatctc
     6001 gacgcggggc gatagttctt ccagcgtcga catcacccga ttgctcatgt ctgcgtaaag
     6061 ctcataattg ctgctaaagc aaaccacgcc acagcgacga aacagatctt tttgtttgaa
     6121 ccagggatcg cccattttaa cacccagcgc ctttgcctca gcgtttcggg cgataacgca
     6181 accgtcatta ttcgatagca caaccaccgg tttaccccat aaatcagggc gaaacaccgt
     6241 ctcacagctg gcataaaacg cgtttacatc acagagagca aacatcagcg catcgcctta
     6301 acgacgtgga tcaccacacc aaagacatcc agcgtatctt cgctactgat ggtaatgggc
     6361 gagtacgcac tgttcatggg aataagctgt accgtcgggc gtagttgcaa ttttttcacc
     6421 gtaaactcgc cgtcaacagc agcgatgaca atatcaccat ggctggcggt aatagcgcta
     6481 tcgacaatca gtaaatcacc gtcactaatt ccaccatcaa tcatagaatc accacttgct
     6541 ttgacgaagt aagtcgcgct gggatgctgg atcaacagtt gattcagatc gatgcgctgt
     6601 tcaacgtaat ctgctgccgg tgaaggaaag ccacactgaa caagatcgct aaatagcgga
     6661 aaagtcacaa tttcgcggag atccgcaggc ttgataaaca acataataat ctgcctgaag
     6721 ttatactgtt tttatataca gtagtctgtt cttgccagca gatcaatact gattcaggct
     6781 atcaatattt gtcgctgcat agactgctga tttttcgttc tcttatcctg tgctcacgtg
     6841 gccttctggc gacgacgctc atccagcaga aatgaaaaat atcacccggc taaaaaatag
     6901 aatagaagca ttcgccataa t
//
LOCUS       DAPHDW010000193         6881 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.196, whole genome shotgun sequence.
ACCESSION   DAPHDW010000193 DAPHDW010000000
VERSION     DAPHDW010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6881)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6881)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6881
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.196"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(71..1207)
                     /locus_tag="RH057_004125"
     CDS             complement(71..1207)
                     /locus_tag="RH057_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3130412.1"
                     /translation="MDLLIVLTYVALAWAVFKIFRIPVNQWTLATAALGGVFLVSGLI
                     LLMNYNHPYTFTAQKAVIAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQAR
                     VDRLQADLMTATHNIKTLRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFS
                     ERDIDDARQNFLAQDALVKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLE
                     QTVIRAPSNGYVTQVLIRPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGD
                     DAEVVFNALPGQVFHGKLTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPND
                     DIDALPDGIYAQVAVYSDHFSHVSVMRKVLLKMTSWMHYLYLDH"
     gene            complement(1210..1572)
                     /locus_tag="RH057_004126"
     CDS             complement(1210..1572)
                     /locus_tag="RH057_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV3130413.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATDPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            2109..4022
                     /gene="mtlA"
                     /locus_tag="RH057_004127"
     CDS             2109..4022
                     /gene="mtlA"
                     /locus_tag="RH057_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HDV3130414.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            4117..5265
                     /gene="mtlD"
                     /locus_tag="RH057_004128"
     CDS             4117..5265
                     /gene="mtlD"
                     /locus_tag="RH057_004128"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HDV3130415.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            5265..5852
                     /gene="mtlR"
                     /locus_tag="RH057_004129"
     CDS             5265..5852
                     /gene="mtlR"
                     /locus_tag="RH057_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HDV3130416.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAFENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISRISLKKAFQK"
     gene            complement(5864..6073)
                     /locus_tag="RH057_004130"
     CDS             complement(5864..6073)
                     /locus_tag="RH057_004130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130417.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            6358..6720
                     /locus_tag="RH057_004131"
     CDS             6358..6720
                     /locus_tag="RH057_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HDV3130418.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLQEMTGFSKTAF"
BASE COUNT         1726 a         1742 c         1760 g         1653 t
ORIGIN      
        1 ctaatgaagt aatatgcaaa aataattagt ccttacaagg agcaaagttc cataatctcc
       61 cccctccccc tcaatgatcc aaataaagat aatgcatcca gctggtcatt tttagcagca
      121 ctttccgcat caccgaaaca tggctgaaat ggtcggagta aaccgccacc tgggcgtaga
      181 tgccgtcggg taaggcatcg atatcatcgt tagggtccag ttcaatggtt cccagcacac
      241 cgtccgtgcc gggcacgacc gttaatgatt gcaatacccc ctgcgcctga taagaaccgc
      301 ctggcacgac aggtaaaata ctagtcagtt tgccgtgaaa cacctgccca ggtagcgcgt
      361 taaacaccac ttccgcatca tcgccgggtt tcagacgtaa cagcgagttt tgccgaaatt
      421 gggcgacaat ttgccgtttt tgctcgggga tgaagaccat caccggacgc agcggcaagg
      481 cagctgcgta tgtacctggg cggatcagta cctgagtaac gtagccattg ctcggcgcgc
      541 gaatgacagt ctgctcaagg ttatattttg cttcagtaag ttgcgctctt aagctcacaa
      601 tctgcgattg ctcgccgtta accatactgt cgagctggct ctggatctgc gcctgctccg
      661 ccaccgagcc tttcaccagc gcatcctgcg cgaggaaatt ttgccgcgca tcgtcgatgt
      721 cacgttccga gaacggattc accgccgcct ggctgccttt caagtaacgt tgataatttt
      781 taaacagacg gtcgcgctcc gctgaaacct gggtggtgtt ggcctgcgct tcagtgagct
      841 gcgcacgcag cgtctttata ttatgcgtcg ccgtcatcag gtcagcctga agtctgtcaa
      901 ctcgcgcctg gtaacgaacc gggtcgagct taaaaagcac ctcgcccttt tgaataagct
      961 gattattctt gtcagtgact tcagtaacaa ttcccgtcac ctgtggcgtg atagggatcg
     1021 ctatcactgc cttttgcgcg gtaaaagtgt aagggtggtt gtagttcatc aacaaaatca
     1081 aaccactcac cagaaacacg cctcccagcg ccgccgtcgc cagcgtccac tgatttaccg
     1141 gaatgcggaa gattttaaag accgcccacg ccagcgccac gtaagttaaa acaatcaata
     1201 gatccataat tagatctccg ggaacgtgga atagtcagtc tttttctcgg cggcgagttg
     1261 atgctccagc cgggaaattc ggtcagaaag tgcggcgatt tccgggtcag tcgctttctc
     1321 ctgcgacgca acatgtgact gcatacccca gccacgctcc ggttgataga gcgtcgccca
     1381 gatccacaga aacggccaga taacatgcag agtaaacagg ctcacccagc ccgccgtatg
     1441 aatagcgtcg gcatggggat ggttgcgctt tttggcaatc agataaggga tgtcatgtat
     1501 cgcgatgatt ccataaaaaa tcaccagaaa gacgaagatc agcactccca acgcgaaata
     1561 gtttagaaac atatctgcct cggattcacg tttatcagtg ttgtttttgg gctggcagcc
     1621 agaagggagt caggctgata ttttgacaat aatccgggtt cgcgattctc gccataacac
     1681 caaagaataa tttttagagg tgatgagttg cttagttaca taacgattgt atgacgaagg
     1741 cataacatgc tgtagatcac atcaggtgaa cgccgtaaga aaatatcttg tgattcagat
     1801 cacaaagatt caacaaacca tcaaaacaaa aatgtgacac tactcacatt taaatgccat
     1861 ttttagcgaa aatcgccgcc ttgttgcttt tttacacaag cgttttgtga tgaacgtcac
     1921 gtcaattacc tctctacccc ctatatttat gtgattgata tcacacaaaa ggccgtcgac
     1981 tggacagtta accgattcag tgccagattt cgcagtatct acaaggtccg gctacctctg
     2041 ccgccacatt aacaaaaaac ctcgggcttc cagcctgcgc gacagcaaac ataagaaggg
     2101 gtgtttttat gtcatccgat attaagatca aagtgcaaag ctttggtcgt ttcctcagca
     2161 acatggtgat gccaaatatc ggcgcgttta tcgcgtgggg tatcatcacc gcgttattta
     2221 ttccaacagg gtggttaccg aacgagacgc tggcgaagct ggtcgggccg atgatcactt
     2281 atctcctgcc gctgctgatc ggttataccg gtggtaagct ggtaggcggc gaacgtggcg
     2341 gcgtagtcgg tgccatcacc accatgggcg ttatcgtcgg cgcagacatg ccgatgttcc
     2401 tcggttctat gattgcaggt ccgctgggcg gctggtgcat taagcacttc gaccgctggg
     2461 tagacggtaa gatcaaatcc ggttttgaga tgctggtgaa taacttctcc gcaggcatca
     2521 tcgggatgat cctcgctatt ctggcattcc tcggcattgg cccgattgtt gaagccctgt
     2581 ccaaaatgct ggctgcgggc gttaacttca tggttgtcca tgacatgctg ccgctggcgt
     2641 ctatctttgt tgaaccggcg aaaatcctgt tcctcaacaa cgccattaac cacggtatct
     2701 tctcgccgct gggtattcag cagtcccatg aactgggtaa atcaatcttc ttcctgattg
     2761 aagctaaccc aggtccaggt atgggcgtgc tgctggcgta catgttcttt ggtcgtggta
     2821 gcgctaaaca gtctgcgggc ggtgcggcaa tcatccactt cctgggtggt atccacgaaa
     2881 tctacttccc gtatgtgctg atgaatccgc gtctgatcct cgcagtcatc ctcggcggta
     2941 tgactggcgt gttcacgctg actatcctgg gcggtggtct agtttctccg gcatctccgg
     3001 gttctatcct tgctgtactg gcgatgacac caaaaggtgc ttacttcgct aacatcgcgg
     3061 gtgtgtgtgc ggcgatggct gtctccttcg ttgtctctgc tattttgctg aaaaccagca
     3121 aagtgaaaga agaagatgat attgaagcag caactcgtcg tatgcaggac atgaaagctg
     3181 agtctaaagg cgcatctccg ctgtctgctg gcgatgtgac taacgacctg agccacgtac
     3241 gtaaaatcat cgttgcctgt gacgccggta tgggttccag tgcgatgggc gcaggcgttc
     3301 tgcgtaagaa aattcaggat gcaggtctgt cgcagatttc tgttactaac agcgcgatca
     3361 acaacctgcc gccagatgtg gacctcgtca tcactcaccg tgacctgacc gaacgcgcta
     3421 tgcgccaggt tccgcaggca cagcatattt cgctgaccaa cttcctcgac agcggcctgt
     3481 acaccagcct gaccgaacgt ctggttgctg cccaacgcca cacggcaaac gaagagaaag
     3541 taaaagacag cctgaaagac agctttgacg attccagtgc taacctgttc aagctaggcg
     3601 cggagaacat cttcctcggt cgcaaagcgg caaccaaaga agaagcgatt cgttttgctg
     3661 gcgagcagct ggtgaaaggc ggttacgttg agccggaata cgttcaggcg atgctggatc
     3721 gtgaaaaact gaccccgact tatctgggtg agtctatcgc ggtgccacac ggtacggttg
     3781 aagcgaaaga tcgcgtactg aaaacgggcg tcgtgttctg ccagtacccg gaaggcgtgc
     3841 gcttcggtga agaagaagat gacattgccc gtctggtgat tggtattgca gcccgtaaca
     3901 acgagcacat tcaggttatc accagcctga ccaatgcact ggatgatgag tccgtcatcg
     3961 agcgtctggc acacaccacc agcgtggatg aagtgctgga actgttggca ggtcgtaagt
     4021 aatccaatcc taccctctcc tcacggagag ggtttgggtg agggaaaagc ctcaccccag
     4081 ccctctcggg taaaaacatt gatgaaggtt aatactatga aagcattaca ttttggcgca
     4141 ggtaatatcg gtcgtggctt tatcggtaaa ctgctggcag acgcgggtat ccaactgacg
     4201 tttgccgatg tcaatcaggt ggtacttgat gccctgaatg cccgtcatag ctatcaggtt
     4261 catgtggttg gtgaaaccga gcaggtagat accgtttccg gcgtcaatgc tgtcagcagc
     4321 attggtgatg atgtcgttga tctgattgct caggtcgatt tagtcactac cgccgttggc
     4381 ccggttgtgc tggaacgtat tgctccggct atcgccaaag ggctggtgaa acgcaaagaa
     4441 caaggtaatg aatccccgtt gaacatcatc gcctgtgaaa acatggtacg cggtaccacg
     4501 cagctgaaag gccatgtgat gaacgccctg ccggaagacg ccaaagcgtg ggtagaagaa
     4561 cacgttggct ttgtcgattc cgccgttgac cgcatcgtac cgccttcggc ttcggcaact
     4621 aacgatccgc tggaagtgac ggtagaaact ttcagcgaat ggattgtcga taaaacgcag
     4681 ttcaaaggcg cactgccgaa catcccaggc atggagttaa ccgacaacct gatggcattt
     4741 gtcgaacgta aactcttcac cctgaacacg ggtcatgcta taaccgcgta cctcggaaaa
     4801 ctggccggtc atcagaccat tcgtgacgct attctcgacg agaaaatccg cgcggtggta
     4861 aaaggtgcga tggaagaaag tggtgcggta ctgatcaagc gctacggctt tgacgcagac
     4921 aaacatgcgg cgtacatcca gaaaatcctc ggtcgttttg agaacccgta tctgaaagat
     4981 gatgtagagc gcgtaggccg tcagccgctg cgtaaactga gtgctggcga ccgtctgatc
     5041 aagccattgc tcggtacgct ggaatacagc cttccgcaca agaatctgat tcaggggatt
     5101 gctggtgcaa tgcacttccg cagtgaagat gatccgcagg ctcaggaact ggcagcactg
     5161 atcgctgaca aaggtccgca ggcggcgctg gcacagattt ccggtcttga tgccaacagc
     5221 gaggttgtat ccgaggcggt aaccgcttat aaagcaatgc aataatggtg gaccaggcgc
     5281 aggacaccct gcgcccgaat aacagattgt cagatatgca ggcaacaatg gaacaaaccc
     5341 aggcctttga aaaccgtgtg cttgagcgtc tgaatgctgg caaaaccgtg cgaagctttc
     5401 tgatcaccgc cgtcgagctt ttgaccgagg cggtaaatct tctggtgctt caggtattcc
     5461 gcaaagacga ttacgcggtg aagtatgctg tagaaccgtt actcgacggc gacggtccgc
     5521 tgggcgatct ttctgtgcgt ttaaaactca tttacgggtt gggcgtcatt aaccgccagg
     5581 aatacgaaga tgcggagctg ctgatggcat tgcgtgaaga actaaatcac gacggcaacg
     5641 agtacgcctt taccgacgac gagatccttg gaccctttgg tgaactgcac tgcgtggcgg
     5701 cgttaccacc gccgccacag tttgaaccag cagactccag tttgtatgca atgcaaattc
     5761 agcgctatca acaggctgtg cgatcaacaa tggtcctttc actgactgag ctgatttcca
     5821 gaatcagctt aaaaaaagcc tttcaaaagt aagcaacgtc tgcttactgc ccctctacct
     5881 gcttcggccg ataaagccga cgataatact ccagacgttg tagatataac ggcacgcttt
     5941 catcgggtat tccggacgga atcgcgttac ggggaggaag ttttttcaga tactcccgga
     6001 acgcctggct tgatgccatg aaatctacgg ctttatcgat aagaagcgga acgttttcac
     6061 ctaatttgcc catgatgata tctccgtatt accccgcgcc gggaatgcgc ggccgccaat
     6121 tttagtttag gttcccggta aagatgacat taaggaaaac gtgctgaatc ctcaataatc
     6181 atccgctgct gtttattatc ttttttctat agatttctta attaatcaat aaattgcgta
     6241 gtttttaagc atgttaatga ttacgttaca gaactattgc aatgtcatca gttgtgaaca
     6301 aagcacctgg tcgcgcatac taggggctat aaatttatct ttatcagaag ccatcacatg
     6361 aaagaagtcg aaaaaaacga aatcaaacgt ctcagcgatc gtctggacgc catccgccac
     6421 cagcaggccg atctgtcgct ggttgaagcc gcagacaaat atgccgagct ggaaaaagag
     6481 aaagccacgc tggaagcaga aattgctcgc ctgcgtgaag ttcatagcca aaaactgagt
     6541 aaagaagcac agaagctgat gaagatgccg tttcagcgcg caattaccaa aaaagagcag
     6601 gctgatatgg gcaagctgaa gaaaagtgtt cgcggactgg tggttgtgca cccaatgacc
     6661 gcgctgggcc gtgaaatggg cctgcaggag atgactggat tctctaagac tgcgttttaa
     6721 gcgcctcaga cgactggttc gatcatatca acccgcatct ggcttgcctt agctggtcaa
     6781 tgcggggact ctcctgtcta ctcgcctaat taccaaacaa ttcactccct ttacttcgat
     6841 ttaccgctgg ttgatattct gaaaatgcat ttttaccgaa a
//
LOCUS       DAPHDW010000194         6822 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.197, whole genome shotgun sequence.
ACCESSION   DAPHDW010000194 DAPHDW010000000
VERSION     DAPHDW010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6822
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.197"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            233..733
                     /gene="tssB"
                     /locus_tag="RH057_004132"
     CDS             233..733
                     /gene="tssB"
                     /locus_tag="RH057_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     small subunit"
                     /protein_id="HDV3130419.1"
                     /translation="MSKKFEGSVAPRERINISYVPKTDGQTAEVELPLNMLVVGDTGN
                     TQETSPLDERQAVSVNKHNFGAVMAEAAIGLNFTVPATLKGSTTDDELNVALNIKSLD
                     DFSPDSVARQVPEVNKLLELREALTALKGPMGNLPAFRTQLQALLENEESREQLLKEI
                     GQVSNK"
     gene            768..992
                     /locus_tag="RH057_004133"
     CDS             768..992
                     /locus_tag="RH057_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130420.1"
                     /translation="MTAHKNISGTYHLSVADILQVVYQVCFSPSVEINQDGVAALITT
                     LDRRISDLLDEIIHFCEFQQSASHWQRVLH"
     gene            1043..2518
                     /gene="tssC"
                     /locus_tag="RH057_004134"
     CDS             1043..2518
                     /gene="tssC"
                     /locus_tag="RH057_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001501045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system contractile sheath
                     large subunit"
                     /protein_id="HDV3130421.1"
                     /translation="MSLQEEELVSSHAGQPEQASSLLDQIMAQTRIQPGSEGYDVARQ
                     GVTAFIASILQSTASAEPVNKLAVDSMIADIDERISRQMDVIIHAPAFQQVESFWRSL
                     KTMVDRVDFRENIKVNVLHVTKQELLEDFEFAPEIIQSGFYKHVYSSGFGQFGGEPIA
                     AVLGAYEFKNTAPDMKLLQYVSAVGAMAHAPFLSSVSPEFMGLNSWTELPNIKDLYAI
                     FEGPAYTKWRALRDSEDSRYLGLTAPRFLLRQPYSPTDNPVKNFNYYEDVSQNHEDYL
                     WGNTAWMLACNIADSFAKYRWCPNIIGPQSGGAVKDLPVHLFETMGQIQAKIPTEVLV
                     TDRREFELAEEGFITLTMRKDSDNAAFFSANSVQKPKHFPGKDAETNYKLGTQLPYLF
                     IINRLAHYIKVLQREQLGSWKERSDLERELNTWIRQYVADQENPPADVRSRKPLRAAK
                     VEVMDVEGEPGWYQVALSVRPHFKFMGANFELSLVGRLDRE"
     gene            2525..2938
                     /gene="tssE"
                     /locus_tag="RH057_004135"
     CDS             2525..2938
                     /gene="tssE"
                     /locus_tag="RH057_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HDV3130422.1"
                     /translation="MIPDRERTTGSLFERMEASSARNRQGGSIHSLRQSIRQNLRNIL
                     NTRSGSCRGAPELGIDEPEGAENFRESMSRAIEQCIERYEPRISHAEVQAVVSSASSP
                     LDMTFHITAWVTFNETHEVLEFDMAPNGSQHYRVD"
     gene            2942..3688
                     /locus_tag="RH057_004136"
                     /pseudo
     CDS             2942..3688
                     /locus_tag="RH057_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308256.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
     gene            3683..4234
                     /locus_tag="RH057_004137"
                     /pseudo
     CDS             3683..4234
                     /locus_tag="RH057_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308250.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            4231..4896
                     /gene="tagO"
                     /locus_tag="RH057_004138"
                     /pseudo
     CDS             4231..4896
                     /gene="tagO"
                     /locus_tag="RH057_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308249.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagO"
     gene            4896..5367
                     /locus_tag="RH057_004139"
                     /pseudo
     CDS             4896..5367
                     /locus_tag="RH057_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308244.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5294..5776
                     /locus_tag="RH057_004140"
     CDS             5294..5776
                     /locus_tag="RH057_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hcp family type VI secretion system effector"
                     /protein_id="HDV3130423.1"
                     /translation="MANISYLSLSGETQGLISAGCSTLDSVGNKAQPEHKDQIMVYAL
                     MHSISRSQNVNHHELIITKPVDKSSPLLAKALSDNEKMAICEFILYRTSKAGIYQPYY
                     KINLSKARISSIDFVTPHAVLEKELEPQERIAFIYEDISWEHTLAGTNAMSKWQDRVQ
                     "
     gene            5820..6734
                     /locus_tag="RH057_004141"
     CDS             5820..6734
                     /locus_tag="RH057_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130424.1"
                     /translation="MAGLRNNNNTQNAQWADYVGDILRGAQPINQLVPQHPYLNDVPL
                     IDELRHQNTHHVILLTLDVAKKILSPITSFDYIHFITTHPSGIKDTLAWLVNAGKLMT
                     EFDDNGKIIFNLNALKYTKASYFEILGEKYIKITTSSPWLLEKLGKYIFSSRAPQVLE
                     LAIGWRGALSESIKGVKFCIWFSVAWRTIEFIMSSERDLVNFLGDFSMDVAKAVIAGG
                     VATAIGSLASFACVSFGFPVILVGGAILLTGIVCTVVLNEIDAQCHLSEKLKYAIRDG
                     LKRQQELDKWKRENMTPFMYVLNTPPVI"
BASE COUNT         1788 a         1653 c         1752 g         1629 t
ORIGIN      
        1 cacctgacgc gaattgcaca ggttcacaca atctatttaa cgcttgcaga atacccctct
       61 ttacgatcga taaaaagtcc ttttcttatt tattttgaag cgtagaatgc ggggtgatga
      121 aggagtggtg attttatttt caccgccatt agggcggcat tgccgatatt tttttgcggt
      181 acaaatatgc cgtgttcatg gcctgtacga gataacatga cggatcactt ttatgagcaa
      241 aaaatttgaa ggcagcgtcg cgcctcgcga gcgtattaat atatcgtatg tcccaaaaac
      301 agatggccag acggctgaag tggaacttcc gcttaacatg ctcgttgtcg gcgataccgg
      361 aaatacgcag gaaacatccc cactggatga acgtcaggca gtttccgtta acaagcacaa
      421 tttcggtgca gtgatggctg aagcagccat tggcctcaat tttaccgtcc ccgccacgct
      481 gaagggaagc acaacggacg atgagctgaa tgtggcgctg aatatcaagt cactggatga
      541 tttttcacca gatagcgtgg cccggcaggt tccggaagtg aataaattgc tggaactgcg
      601 tgaagcactg actgcactga aggggccgat gggtaacctt ccggcattcc gtacccagct
      661 ccaggcattg ctggaaaatg aagagtcccg cgagcagttg ctcaaagaaa tcggccaggt
      721 gagcaataaa taaccaccac tatttcatta aacagggtta tccaggcatg acggcacaca
      781 aaaatatctc cgggacatac cacctctctg ttgcagacat tctgcaggtg gtttaccagg
      841 tatgtttcag cccgtccgtt gaaatcaacc aggatggtgt ggctgcgctg attacaacac
      901 tggatcgccg tatcagtgat ctattggatg agattatcca tttttgtgag tttcagcaga
      961 gcgcgtcgca ctggcagcgc gtacttcact gacggcgaaa agccggtttc agaaacacag
     1021 ctaaaacaga aggattttca gtatgtcttt acaggaagag gaactcgttt ccagtcatgc
     1081 cgggcagcct gagcaggcat cgtcgctgct tgatcaaatc atggcacaga cccgtattca
     1141 gcccggttcg gaaggttatg acgttgcccg ccagggtgtg acagccttta ttgccagcat
     1201 cctgcaatcc actgcatctg ccgaaccggt caataaactg gccgtggaca gcatgattgc
     1261 agatattgat gaacgcatca gccgccagat ggatgtcatc atccatgcgc ctgcctttca
     1321 gcaggtcgag tcgttctggc gctcactgaa aaccatggtg gatcgcgttg atttccgtga
     1381 aaacatcaag gtcaacgtgc ttcatgtcac caagcaggaa cttctggaag actttgagtt
     1441 tgcgccagaa attattcagt ccggatttta taagcacgtt tattcgtccg gtttcggaca
     1501 gtttggcggt gaacccatcg ctgccgtgct gggcgcttat gagttcaaaa ataccgcgcc
     1561 tgacatgaaa ctgctgcaat acgtcagtgc cgtgggcgca atggcgcacg caccgttcct
     1621 gtcctccgtt tccccggagt ttatggggct gaactcgtgg accgaactgc ctaatatcaa
     1681 agatctttat gccatctttg aaggtccggc ctacaccaaa tggcgcgccc tgcgtgactc
     1741 ggaagattcg cgctatttag ggcttacggc accccgcttc ctgcttcgcc agccctattc
     1801 accaacagat aaccctgtta agaactttaa ctattacgaa gatgtcagcc agaaccacga
     1861 agattatctg tggggtaata cggcctggat gctggcatgc aatatcgcag acagttttgc
     1921 caaataccgc tggtgtccaa acattatcgg tccgcaaagc ggcggcgcag tgaaagatct
     1981 gccggtgcat ctgttcgaaa cgatggggca gattcaggcc aaaatcccaa ccgaggtgct
     2041 ggtcaccgat cgccgcgaat ttgaactggc tgaagagggt tttatcaccc ttaccatgcg
     2101 taaagactct gataatgcag cctttttctc tgcaaactcg gtacaaaaac cgaagcactt
     2161 tccgggaaaa gatgcagaaa ccaactataa gctgggtacg cagcttcctt atctcttcat
     2221 catcaatcgg ttagcgcact acatcaaggt gttgcagcgt gaacaactgg ggtcatggaa
     2281 agaaaggagc gacttagaaa gagagcttaa tacctggatc cgtcagtatg ttgccgacca
     2341 ggaaaacccg cctgcagacg tgcgcagccg caaacctctg cgcgctgcca aagttgaggt
     2401 tatggatgta gaaggcgaac ccggctggta ccaggttgcg ctaagcgtga gacctcattt
     2461 taaatttatg ggggcaaatt ttgagctttc cctggttggc cggttagaca gggagtaaca
     2521 caccatgata ccggatcggg aaagaaccac aggttctctt tttgagcgta tggaagcctc
     2581 ttcggccaga aaccggcagg gaggcagtat tcattcgctc aggcaatcca ttcgccagaa
     2641 cctacgaaac attcttaata cccggtccgg tagttgccgg ggtgcgcctg agctgggcat
     2701 tgatgaaccg gaaggggctg aaaacttccg cgaatccatg agtcgggcca tcgagcaatg
     2761 tatagaacgc tatgaacccc ggatctcaca cgctgaagtg caggcagtgg tttcatccgc
     2821 ctccagcccg ctggatatga cgtttcatat tacagcctgg gtgacattta acgaaacgca
     2881 tgaggtgctt gaatttgata tggcaccgaa tggcagccag cattaccggg tggactgaca
     2941 aatggaattt gaagaacgct atttccggga agaactcgat tacctgcgcc agcttagcaa
     3001 gctgctggca acggaaaaac cccatctggc ccgcttcctg gccgaaaaag atgcggatcc
     3061 ggatattgaa cgcctgctgg aaggggtggc ttttcttacc ggcaatctcc gccagaaaat
     3121 tgaggatgaa ttcccagaac tgacgcacgg gcttattaag atgctatggc ctaattacct
     3181 gcgtccggtt ccggcaatga cccttattga atatacgccg gatatggata agtcttctgt
     3241 accggtgtta atcccccgta atgagcagtt tacaaccaac gccggggaaa tcagagttga
     3301 tgaagtgctg ccctctgatg ctaaaaagga ggagccgcct ccctgtacct tcaccctctg
     3361 ccgggatatc tggctgctgc ccgttcgcct ggagcagatt gaaaaccgca gtacgacccg
     3421 taatggtgtt atcaacatca ccttttcggt cgcaccggga acggacttcc gcacgctgga
     3481 tctgaacaaa cttcgcttct ggctcggcaa tgacgacaac tatacccgtg accagcttta
     3541 tttatggttc tgcgaatact tgcagggtgc cgacctgact gtgggtgaac agcatattcg
     3601 cctgcctgag tttatgctaa aagctgtcgg ttttgagccg caggatgcca tgctgccctg
     3661 gccgaaaaac gtccacagcg gctaccaggc gttcgataag ggcgaaatgg cagacggtga
     3721 aggccgcctg attgactgca aaaatatcgt cttcttcctg acgtccaacc tcggctacca
     3781 ggtaatagtc gagcatgccg atgacccgga aaccatgcag gaagtactgt atccggtgct
     3841 ggccgacttc ttcaaacctg ccctgctggc gcgtatggaa gtggtgccgt atctgccgct
     3901 gtcgaaagag acgctcgcca ccattatcgc cggaaaactg gcccgtctgg ataacgtgct
     3961 gcgcagtcgc tttggtgcag aagtggtcat tgaaccggaa gtgacggacg aaatcatgag
     4021 ccgcgtcacc cgcgcggaaa acggcgcgag gatgctggaa tcggtcatcg atggcgacat
     4081 gctaccgccg ctctcgctgc tgctgttgca gaaaatggcg gctaacacgg cgattgcccg
     4141 gattcggttg tcggcagtgg acggcgcatt tacggcagat gtggaagacg ctctggacga
     4201 cgagtccgtc acagaggatg aaccggtttt atgagcaggt tgcgcgtttt ccggcagaca
     4261 ggatggttta tcgcgggcct gatgacgggc ttacccgcca ctgccgcgcc cgcagaagca
     4321 tcgtcaatgg ccggtgtggc ggtcgccgtc gccaccacga ccccgccgga tgcgacagcc
     4381 acgttacagg ccatgcagtc ctgtcggcgg gagtccgcgg cgctggagcg gcttgactgc
     4441 tatgaccgcc tgctggcacc gctgtccccg tcagggtttg acggggcgct ggtcaaagcc
     4501 ggttttgtgg gcgaggcctg gacccgcgcc acagagcagg aaaaacgccg cgagggcaac
     4561 accacagaac tgctggtgac gcaggtgccg ggggagcgcc cgacggtggt gataaccacc
     4621 ccggctatcg gtcatgttcc gccacgcccg gtgctgatgt tcagttgcgt ggacaatatc
     4681 acccggatgc aggtggcgct gatgcaccct ctggatgtcc atgatatcgc cgtcactctg
     4741 aatgccgaca accgggcatt gcgcagccac tggtttgtgc gcgagaacgg caccctgctg
     4801 gagtccagcc gggggttatc cgggattgat gaaattaagc aactgttcgg cgcaaaaacg
     4861 ctgacggtgg ataccggcgc agacaatgcc gccggaaaca gcaacaggcc tcgacacaat
     4921 ggaatgaggc actcaaaacc cgcgcacaga gtagcccgca gttgcgtggc tggttgcaga
     4981 cccgccagga cttacatgct tttgcagatc tagtgatgca gcgcgagaaa gagggactaa
     5041 ccctttccta tatcaaaaac gtcatctggc aggcggagcg gggactgggg caggaaacac
     5101 ccgttgagtc tctgttgacg cagtaccagg atgcccgtgc gcagaagcag aatacagatg
     5161 cgctggaaaa acaaattaat gagcgactcg aaggcgtgtt aagccgctgg ctgctgctga
     5221 agaataacgt catgccagag gcggcaaccg gtaccacggc cgaaaaataa ttatttaatt
     5281 aaaggagatt atcatggcaa atataagtta tttatctttg agtggagaga cacaagggct
     5341 gatttcagca ggttgctcaa cgcttgattc tgtcggaaac aaagcgcagc cagaacataa
     5401 agatcagatc atggtttatg cgttaatgca ttccatctca agaagtcaaa atgtcaatca
     5461 tcatgaatta attatcacca aaccggttga taaatcctca ccattgctgg caaaagcact
     5521 tagtgacaat gaaaaaatgg caatatgtga atttatactc taccgaacca gtaaagcggg
     5581 tatttaccag ccttattata aaataaatct gtcaaaagcc cgaatatcaa gtattgattt
     5641 tgtcacaccg catgctgtcc tggaaaaaga actggaacca caggagagaa ttgcctttat
     5701 ttatgaagat atttcctggg agcatacgtt agccggtacg aatgcaatga gtaaatggca
     5761 ggatcgtgta cagtaactta ctgtaataac ataataaatt actattgcgg agagataaaa
     5821 tggctggttt aaggaataac aacaacacac agaatgcaca gtgggctgat tatgtgggtg
     5881 acattcttcg gggggctcag ccgataaacc agctagtacc tcagcaccct tatcttaacg
     5941 acgtcccact cattgatgag ttaaggcatc agaacactca tcatgttatt ctcttgacac
     6001 ttgatgttgc aaaaaaaata ctcagcccaa taacatcatt tgattacatt cattttatca
     6061 ctacacatcc ttcaggtata aaagatacac tcgcctggtt agttaacgcg ggtaaattaa
     6121 tgactgagtt tgatgacaat gggaaaatta tatttaacct taatgcctta aagtatacaa
     6181 aagcaagtta ctttgaaata cttggagaga aatatataaa aatcacaaca tccagcccct
     6241 ggcttttaga aaagctcggt aaatatattt tctcttcacg agcgcctcaa gttctggaac
     6301 tcgctattgg ctggcgagga gctttatctg aatcaataaa aggagttaag ttctgtatct
     6361 ggttttcagt agcctggaga accatagaat ttattatgag ttcagagcga gatctggtta
     6421 attttcttgg cgatttttca atggatgtgg ccaaagcagt tatagccggt ggtgttgcaa
     6481 ccgctattgg aagcctggct tcttttgcct gtgttagctt tggctttcca gtaattcttg
     6541 tcggaggagc aattttactg acagggatag tgtgtacagt tgttttaaat gaaatcgatg
     6601 ctcaatgcca tttatcagaa aaattaaaat atgcaattag agatggacta aaacggcaac
     6661 aggaacttga taaatggaaa agggaaaaca tgactccatt tatgtatgtt cttaacactc
     6721 cacccgtgat atgaggatta accatgcgtt tacatgttaa attaaaagag tttctttcga
     6781 tgttttttat ggccatatta tttttccctg cattcaatgc tt
//
LOCUS       DAPHDW010000195         6494 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.198, whole genome shotgun sequence.
ACCESSION   DAPHDW010000195 DAPHDW010000000
VERSION     DAPHDW010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.198"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..323)
                     /locus_tag="RH057_004142"
     CDS             complement(<1..323)
                     /locus_tag="RH057_004142"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130425.1"
                     /translation="MSELKVISHAMFNISVEQAEASRVDLEHGEGDFQKYCGQLLDEL
                     LENTRSKSFKFRAIEEFVPAHLNVLVNDQNEWDKRTLGIAEKLLSVEIDAQDKIKAMK
                     KKIKKG"
     gene            709..1329
                     /locus_tag="RH057_004143"
     CDS             709..1329
                     /locus_tag="RH057_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3130426.1"
                     /translation="MRVWAYLRASTKEQDADRALKELEAFAHSHDLKISKYFKENESG
                     ASLQRPQLFLLLDIAERGDILLCEQIDRISRLTAEDWKTLRGLIESKGIRVVSLDLPT
                     SHQLLHVQDEFTVRMFEAMNSMMLDMLAAISRKDYEDRRRRQKQGIEKAKREKKYRGR
                     PVDESLHHKVQELLSDGKSWSKIQALIGCSRATIAKVAKNSSLTAE"
     gene            1547..1774
                     /locus_tag="RH057_004144"
     CDS             1547..1774
                     /locus_tag="RH057_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HDV3130427.1"
                     /translation="MGKDQHVVPHDGGWAVKGAGNSKATSVHSTKQSAVDAARGIATN
                     QKSELVIHNKDGRIAKKDSHGNDPCPPKDKK"
     gene            2118..2471
                     /locus_tag="RH057_004145"
     CDS             2118..2471
                     /locus_tag="RH057_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 family protein"
                     /protein_id="HDV3130428.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAATQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(2513..4063)
                     /gene="pdeB"
                     /locus_tag="RH057_004146"
     CDS             complement(2513..4063)
                     /gene="pdeB"
                     /locus_tag="RH057_004146"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308537.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HDV3130429.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPEPGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FPEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDWLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(4227..4697)
                     /locus_tag="RH057_004147"
     CDS             complement(4227..4697)
                     /locus_tag="RH057_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HDV3130430.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(4813..5364)
                     /gene="maa"
                     /locus_tag="RH057_004148"
     CDS             complement(4813..5364)
                     /gene="maa"
                     /locus_tag="RH057_004148"
                     /EC_number="2.3.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
                     /protein_id="HDV3130431.1"
                     /translation="MSTEKEKMIAGELYRSADETLSRDRLRARQLIHRYNHSLAEEHT
                     LRQQILADLFGQVTEAYIEPTFRCDYGYNIFLGNNFFANFDCVMLDVCPIRIGDNCML
                     APGVHIYTATHPIDPVARNSGAELGKPVTIGNNVWIGGRAVINPGVTIGDNVVVASGA
                     VVTKDVPDNVVVGGNPARIIKKL"
     gene            complement(5535..5753)
                     /gene="hha"
                     /locus_tag="RH057_004149"
     CDS             complement(5535..5753)
                     /gene="hha"
                     /locus_tag="RH057_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HDV3130432.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(5779..6153)
                     /gene="tomB"
                     /locus_tag="RH057_004150"
     CDS             complement(5779..6153)
                     /gene="tomB"
                     /locus_tag="RH057_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HDV3130433.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQDLQKWRK
                     SGNRLFRCFVNATKENPASLSC"
BASE COUNT         1860 a         1398 c         1479 g         1757 t
ORIGIN      
        1 ccttttttaa tttttttctt catagcctta attttatctt gagcatctat ttccacggac
       61 aaaagttttt cagcaattcc aagtgttctc ttgtcccact cgttttgatc attaacaagc
      121 acgttaagat gagcagggac aaattcttct attgctctga atttaaagga tttgctgcga
      181 gtattttcca gtaactcatc taacaattgg ccgcaatact tctgaaaatc gccctcaccg
      241 tgttcaaggt ctaccctact tgcttctgcc tgttctacgg atatgttaaa cattgcgtgt
      301 gatataactt taagttcact cattgctgat aagtccttat aagaaatcaa atcacagcac
      361 tggttattca tacagtatat tccgaatgaa tctatctacc agcaccttta caaaaaatta
      421 gaagaggttg tcactaacat aaacactgcc gactgcgata catctaatta aacatgaaaa
      481 attctctata agccagacag ttagctcgat ttgtctatgc gtaaatggat attatttcat
      541 tggaaatagt aacttattgt tattgatgtg cagtattcct agtgcatcat atgtatacct
      601 ctagcagacc tacacatatc taattcaaaa tgattcatta aatggatttt acaacctaaa
      661 acagaccata tataatgaac catataaaac agactgatgg tgactatcat gcgtgtatgg
      721 gcatatctaa gagcttcgac gaaagagcaa gatgcggata gggcattgaa agagttggag
      781 gcatttgctc attcgcatga tctgaaaata tcaaagtact ttaaagaaaa tgaatcaggt
      841 gcaagcctac agcggcctca attattttta ctcttagata tcgcggaacg tggcgatatt
      901 ctgctctgtg agcaaataga tcgtataagc cgacttaccg cagaggactg gaaaacctta
      961 cgaggactca tcgaatctaa agggatcagg gttgtatcgt tagatttgcc tacgagccat
     1021 cagcttttac atgttcagga tgagttcacc gttcgtatgt ttgaagcaat gaattctatg
     1081 atgctcgata tgctggcggc aatcagtcgc aaggactacg aagatcgccg cagacgacaa
     1141 aagcagggta ttgaaaaggc caagagagag aaaaaatatc gtggtcgccc agttgatgag
     1201 agcctgcatc ataaagtaca agaattactg tctgacggta aaagctggag caaaattcag
     1261 gctttgatcg gttgttcaag ggctactatc gcaaaggtgg ctaaaaactc atctcttaca
     1321 gcagaatagt gggtaacaaa gggcagagta ggccagcact gccagattgt taacgaatat
     1381 agcggtatag ggttaaggaa gggtacttac agtttcgtag tgaaagaatc ctttcgatat
     1441 ctatccacgt tttgtgatta aaatcaattt aaacacaata tgtggtattg agtggttggt
     1501 tttgtcgcta tatgctgatg tttccatgga aatagagggt tcgataatgg gtaaagatca
     1561 gcatgttgtt cctcatgatg gaggttgggc cgttaagggg gcaggaaaca gtaaagcgac
     1621 ctccgttcac tcaacaaagc aatctgcggt ggacgctgca agagggattg ctacaaacca
     1681 aaaaagtgag cttgtgatcc ataacaagga tggcaggatc gcaaaaaaag attcgcatgg
     1741 taacgatcct tgtccaccaa aggataagaa gtaattgaat aaggggcttg tcgccccttt
     1801 tttatgtaca tcgggtaggc acttccgaca ctataaattt cgaaaagtgc atgtcatgtc
     1861 cccaaccatg acggcacgtt accttgcaag attttgtgct ggacggtact atctagagtg
     1921 gtacagtact gttgtagtac taccactata accaattgat taaccaacac aatttagtac
     1981 taatggctga tggcagggcc cccacccatt tctgcctccc accgtttcgt caaaaaaccc
     2041 caacatggct aaactttaac cacaactgac gtcgcaagaa ttgtctggct gcgcagtacg
     2101 cttcggaggt atgtctgatg aagtatgttg atggttttgt ggttgccgtt cctgccgata
     2161 aaaaggatgc ctatcgggaa atggccgcta aggctgcgcc attgtttaaa gagtttggcg
     2221 cgcttcgtat tgtcgaatgc tgggccagcg atgtaccgga tggcaaagtg accgattttc
     2281 gtatggcggt gaaagcggaa gagaatgaag aggttgtctt tagctggatt gaataccctt
     2341 caaaagaggt ccgcgacgct gctactcaaa agatgatgtc ggacccacgg atgaaagagt
     2401 tcggcgagtc catgccgttt gacggcaagc gaatgatcta tggcggattc gagtcaatca
     2461 tcgacgaata ggtagcgtga cgggctgtgc ggcgtgccgt cagcccaaaa attcacaaat
     2521 gttgctcggc ccagcgtaag aaatcttctt tcggtaatgc cttgctgtag agccagccct
     2581 gaccgtagtg cacgccatgc tggcgtagcc actcttcttg tttactggtt tcgattccct
     2641 ccgctaccat tttcagtttc agtgtttttg ccatttcgat gatatgcggc gtgacatttt
     2701 tatattccag cgcatcaacg aaagatttat cgatcttcag aatgtcgaca tccagatcct
     2761 gtaaataact taaacttgaa tatcccgtac caaaatcatc aagataaatt tcatggcccg
     2821 cctcccggta gcgagaaatt atcggggcgc tggttttcgg atcggcaaac tcgcgttcag
     2881 tgagttcaag cgcgatctgt ctgggattaa cctgatagtg attgatcatt tcacgcagca
     2941 attgcgggat tttttccgag gtgagcacgg tggattcaag attgatcgaa atatgctgct
     3001 gtggatgctg acgcagccag tcgcccatat cttcaaagac gcttcttata atcagtagcg
     3061 tcaatggctc agaaaggccc gtttgctgtg ccagcggaat aaaactatct ggtgacaacc
     3121 aactaccgtc tgtctgcggc cagcgcgcca gtgcctcagc accgacaatt ttgccattgg
     3181 ctaaggagac aatcggctga tagtgcacgc aaatatcacg attttcgata gcatcctgca
     3241 gccgatgatg cggtgactgg atacggcgca aaatacgcag cacaaacatc gctgccagca
     3301 ggccaatcac caacccgagc ggtaaccaaa taaagacttg ccgatgccag cctttctgca
     3361 acattttcgt tgaagcccag gtgatgatcg aaatattcat ctccgggaag ggcaggatat
     3421 cgtaaatgat tccattattt tcgatatgct caccgggtgt tttttgtagc ctggtaataa
     3481 ttccctgagc aatttcatcg ctgctggtta tgacaacgtt atgggcattg ccaataatgg
     3541 cggcatcaat ttgccatgag ctatagggaa tgacatcaat aaaggaagcg gggtcgatca
     3601 tgacgacata atgtgccgtt cccatggcga ccatgtaacg gataatgcct aaatcgttat
     3661 gcgatgttaa ccagacacga taaccatctt tcgaaatttt acctggctcg gggaaggtat
     3721 cgggcggact ttcatgctcc agagacgaac actggggaac gttgttatcg atataaacca
     3781 cttcctgaat atagcgataa ctgtaagata cccgacgcat ttccatgaga tgggcttcgc
     3841 tacaggcagc gccttgccat ctttccagct cttgcagcgc atctttccct tgtgtcgcca
     3901 ccttattggc ccgaatagcg acgcgggagg aataggtatc cagctcttca ataaacgatg
     3961 tttctacctg ctgatgggcc agccagatgc ttaagccgac aggcagcaat actgaaagaa
     4021 tcagtactcc cgaaataagg ccgaccagat gtcgtgttct caccagcata tccttattaa
     4081 cacagtgtag ggaaagtata tctgattgtg gaaaaaggct ggcaggagat aaggaggtag
     4141 agctggtatt tcatgaagtg atgggtggaa acgatcattg tcatttgacc aggcgcagag
     4201 ctttacgcct ggtatgtaac cctaacttaa gatgttgatt cggcgcgagc gagggtaaaa
     4261 atatcgtaaa aagacagttc acctttacgg acgatcattt tttgcagttg ttccttatga
     4321 acatccgcga cacgcggaga gttcaagatg gcttcaacca gcgcggcagg tacaaagcgc
     4381 gtgttgtacc attcgccgtt atagcaaacg cggaaatcca gcacgtcgat atcttcatag
     4441 cggtagcgtg ggtagacatc gaaaaagaag aaaccattaa gcaggataaa taatacaacc
     4501 agtagccaga cagagcctga cagcgtttcg gactgcaaca tcaccgccag ggtggcaaaa
     4561 aaagcaacgt acataccgat aaacagcccc ggatgtttac ggataaaact gatactaaag
     4621 cggggtttgt tgtcgcgttt ttcgcgggta ttcagagact caatcgtttc ggtcagcagg
     4681 cgttgtattt cggtcatcgt ttgccttcgt ggtatctgca actttccagg gaacacccta
     4741 ttttaacgtg ggaatagtag agaggaagta acagatttac cacaattttg cataacagtt
     4801 gcgaaaaacc gattacaatt ttttaattat tctggctgga ttaccgccca cgacaacgtt
     4861 gtccgggaca tcttttgtga caactgcacc tgaggctacc acgacgttat caccaatggt
     4921 cacaccaggg ttaatgaccg cgcgtccgcc aatccagacg ttattaccga tggtgacggg
     4981 tttccccagt tcagcaccgc tattacgtgc tacagggtcg atgggatgtg ttgccgtgta
     5041 gatatgaacg cctggtgcca acatacagtt atcaccgatg cgaatagggc agacatcaag
     5101 catcacgcaa tcgaagttgg cgaaaaaatt attaccgaga aaaatgttat agccataatc
     5161 acagcgaaac gttggctcaa tataagcctc tgtcacctga ccgaatagat cagcgagaat
     5221 ttgctggcgt aatgtgtgct cttccgccag ggaatgattg tatcggtgaa taagctgacg
     5281 agcgcgcagg cgatcgcgag ataacgtctc atctgccgag cgatacaact caccagcaat
     5341 catcttttct ttttctgtgc tcattactga cctccggttg ctgaaaatgc ggcaacacag
     5401 taagacgaaa agtcagtgaa gagaacgttc ccattacatc tgtgtgataa agatcacata
     5461 gggtatgtct tcatggtgaa aagtataaaa ttcttaataa acagccggtt atagctccga
     5521 aagcgaatta tttattagcg aataaatttc catactgagg aagggatctt gtcgtatagt
     5581 ttattcatgg tcaattcggc gaggcggtga tctgcggctg agtaaaatac cgccagttca
     5641 ttatctgata attcgtattt atttttctcg ataacacgct ccagcgtgtc aattgtctgg
     5701 caacgacgta aacgcattaa ataatcggtt ttcgttaaag gtttttcgga catacttcta
     5761 cccatggttg taataattct aacaagataa actcgcagga ttctctttcg tcgcattgac
     5821 aaaacaacgg aatagtcgat tacctgactt ccgccatttc tgaagatcct gcatattaat
     5881 accataacta ctgaacaaca taaaggtgtc atccagatat tcgtcgatct gctcaatgag
     5941 cttattgtct tcattatact taattttgta attaagtgcg aaggtcgcaa tatgctcaat
     6001 cagttcattc aactggaggt tgatcgccga ggttgggtcg ttaacccagc catgattgct
     6061 ttcttcaagg tttgcaaggc agtcatgata cagggtttca cagagaaact taagctgtgc
     6121 gatatcatgt cttttgggtg agtattcatc cataacgcgt ccccttctta gcggttgaac
     6181 taacggacac ctttcgggat ggaaaaaact tactgacctg gacttgccct ctcgtttgtt
     6241 agcttaacta taagccactc tttgcaggtt ttcatcgcat tattacgaaa aattacaatt
     6301 aggaaggaat cgggacagga ctcacttttt catactagca ctaacccgca gcaggtaaaa
     6361 gcagtctgct cgaactgttc ataacatttc aggattatcg ccagatgaaa ttggtgacac
     6421 taataccagg attgctctga atatgacgta ataaccgagg aatgaataaa gaattaccgc
     6481 aaataattaa gaat
//
LOCUS       DAPHDW010000196         6431 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.199, whole genome shotgun sequence.
ACCESSION   DAPHDW010000196 DAPHDW010000000
VERSION     DAPHDW010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6431
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.199"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..347
                     /locus_tag="RH057_004151"
     CDS             <1..347
                     /locus_tag="RH057_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130434.1"
                     /translation="IFAAIQKNESLLKFPSIISMLNINLLFNPLNLPGMGSGVLEDIM
                     SIPDSSLRKRLGYEVLSFSLQAHSLSQECIDKLDIFFAGDLFKYESVCIAAMEHLKSK
                     ATAPIQNGPLPA"
     gene            complement(351..1742)
                     /gene="tcyP"
                     /locus_tag="RH057_004152"
     CDS             complement(351..1742)
                     /gene="tcyP"
                     /locus_tag="RH057_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HDV3130435.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     LSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLAA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAASIPLNVEAQTRRLGVPESIASFA
                     ASFGATIGQNGCAGLYPAMLAVMVAPTVGINPLDPMWIATLVGIVTVSSAGVAGVGGG
                     ATFAALIVLPAMGLPVTLVALLISVEPLIDMGRTALNVSGSMTAGTLTSQWLKQTDKA
                     ILDSEDDAELAHR"
     gene            complement(1875..2465)
                     /locus_tag="RH057_004153"
     CDS             complement(1875..2465)
                     /locus_tag="RH057_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HDV3130436.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            complement(2628..3296)
                     /gene="hxpB"
                     /locus_tag="RH057_004154"
     CDS             complement(2628..3296)
                     /gene="hxpB"
                     /locus_tag="RH057_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HDV3130437.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            3443..3979
                     /locus_tag="RH057_004155"
     CDS             3443..3979
                     /locus_tag="RH057_004155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HDV3130438.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFTHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            complement(4020..4880)
                     /locus_tag="RH057_004156"
     CDS             complement(4020..4880)
                     /locus_tag="RH057_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HDV3130439.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACYWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4986..5276)
                     /gene="ghoS"
                     /locus_tag="RH057_004157"
     CDS             complement(4986..5276)
                     /gene="ghoS"
                     /locus_tag="RH057_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HDV3130440.1"
                     /translation="MASGDLVRYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            complement(5377..6306)
                     /gene="pfkB"
                     /locus_tag="RH057_004158"
     CDS             complement(5377..6306)
                     /gene="pfkB"
                     /locus_tag="RH057_004158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HDV3130441.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
BASE COUNT         1671 a         1596 c         1570 g         1594 t
ORIGIN      
        1 gcatttttgc tgcgattcaa aaaaatgaga gtctcctaaa gtttccttcg ataatatcaa
       61 tgcttaatat caatcttctt tttaatccac taaatcttcc gggaatggga agtggtgtgc
      121 ttgaagatat tatgagtatt ccagatagtt cacttagaaa acgtctcggg tatgaggttt
      181 taagcttctc gctacaggca cattcattga gccaggaatg tattgataag ttggacattt
      241 ttttcgctgg cgatctgttt aaatatgagt cagtatgcat cgccgcgatg gaacatttaa
      301 aaagtaaggc aacggcccct atacaaaacg ggccgttgcc agcataagaa ttaacggtgt
      361 gccagttcgg cgtcgtcttc gctatccaga atggctttat cggtttgctt cagccactgg
      421 ctggtcagcg tgccagctgt catcgagcca ctaacgttta gcgccgtacg gcccatgtcg
      481 ataagcggtt caacggagat taacagcgcc accagggtta ctggcaggcc catcgcaggc
      541 agcacaatca gtgcggcgaa agttgcacca ccaccgacac cggcaacgcc tgcggaacta
      601 acggtaacaa taccgaccag cgtcgcaatc cacatcgggt ccagcgggtt aatgccaacc
      661 gtaggcgcaa ccatcaccgc cagcattgcc ggatacaaac cggcgcagcc gttctgacca
      721 atggttgcac cgaaagaggc ggcgaaactg gcgatggatt caggaacgcc cagacgacgc
      781 gtttgtgctt ccacattcag tgggatagac gcagcactgg aacggctggt aaaggcaaac
      841 gtcagcacag gccatacctt acggaagtac ttcagcggac tcacgccatt aatgcccagc
      901 agaatgccat gcactgcaaa cataatcagc agaccgaggt aggacgcgac aacgaaactt
      961 cccagtttga tgatgtcttg caagttagaa cctgcaacca ctttggtcat tagagccaga
     1021 acgccgtaag gggtcaactg catgacaagg cgaaccagtt tcatcaccca gctttgtagg
     1081 gtatcgatag cggctaagac gcgttcacct ttcggcgcat catccttcag cagtttcaat
     1141 gcagctacgc cgaggaatgc ggcaaaaatt accacgctga taattgacgt cggattggct
     1201 ccggtaagat cggcaaacgg gtttttcggg ataaaggaca agaccagctg cggaacgctc
     1261 agatcagaga ctttaccaac atagttactt tcaatggcgt tcagacgtgc agtttctgca
     1321 ccaccctgaa ccagaccttc agccgtcaaa ccaaacaggt tggtgaccag cacaccgacc
     1381 agcgccgcaa tcagcgtggt aaacaaaagc gtaccgatgg tcagaaaact gattttgcct
     1441 aactgagatg cgttatgcag acgggcaacc gcgctcagaa tagaggcgaa gactaacggc
     1501 ataacgatca tttgcagcag ttgaacatag ccgttaccaa cgatgttaaa ccactgtaca
     1561 gaatctttaa gtacctggct gtcagaacca taaatggtat gcagggcaag gccaaaaacc
     1621 acacccatca ccagacccac cagcactttt ttcgccagac tccactgttt atggcgggtc
     1681 tgagccagcg caaacagcag tacaacgaac accacgatgt tcgcaattaa tggaaagttc
     1741 atccccgttc tcctgatctt attatttcta tcactgcgta gcagtcatag tgttggcgca
     1801 agattaacag aactgtgagc tggctcttat attcaaatgg aattgcttat tccagaaagg
     1861 tgatttttgc ctttttagta ttcaacctga tgcttaataa gccgatgaaa ctggatctgc
     1921 aaggtattga tcatttgcga tgaccaacga acagcgctgt tctcgggtaa tgaatggggc
     1981 atccataccg accagacaaa taatgccatg cagataaaac gttccagttg gttgcccttt
     2041 tggggaacca ggataggcaa gcggaaacgc cagcggcatg gccagagcag gggaacaccg
     2101 gcgggtgtca gcatatcggc aagtatgtgg ctcaaataac ccagcaccat tccttgtagt
     2161 gcatcagccg gaatgaacca gccttccgga accttaaggt aaaaggttgc cagcagcgca
     2221 aataccgcca gcagactgtg ggtaaaacca cgatgcccaa aagcgcgggc gatcggtttt
     2281 gatatccatt ttaatcgctg cccaagaaac gactttgggt gatcgatgtc cggtaacaaa
     2341 cacgtcagga ttgcggaagg gacaatatgc caccagtcac cctgtgccag cacgggcgtc
     2401 agctcggcat ttttggcaaa taccgcacaa gcaatagaaa agagaaggtg accttccgcc
     2461 gtcatgataa aactcactaa actgtcgata cgtacagtat aggattttta tacagtgcac
     2521 ggaagtggtg acggtgtaac tctttctctt aaattgacga ttaatccgtc agttgaatgc
     2581 aaattcagat aaagaaccag gggcagcgtg atcactgccc ctggtcatta accgagaagg
     2641 tcttttgcgg tgagttctgt cagcgatgag agtttgacgt ttgctaatac aaaacgtgga
     2701 tcattttgcg cttctggcgc aggaacgacg atggaacgca tgcgggctgc tttagaagcg
     2761 atcatgccat ttaccgagtc ttccagcgcc acgcaggtca gtgggtcaac gcccagtttt
     2821 gctgcgcagt cgagatatac ttgcggatgc ggcttgctgt aaggcagttt ttcagccgag
     2881 gcgagggcat cgaaactgtc gcgtaagtca aacatggtca acactttttc cagcatatgt
     2941 agtggtgacg cggaggccag tcccaccaat aaaccttgtt ctttgcataa cgcaacagct
     3001 tcgcgcacgc ctggtaataa tggacgtgtc tcttcaacca gtgaaatggc acgggcaata
     3061 acccgttcta ctacttcctg acggcttggc ccattccacg gttgccgggc gtaccaaaga
     3121 tcgaccacca tatcgatgcg taaacctaag gtgtccggca gctcgttacg acgggagata
     3181 tccaccccca ggcttgccat cacatccagt tcggctcgat cccataaagg ttctgagtcg
     3241 ataagtaatc catccatatc aaaaattgca gcaagaatct gacgcggggt tgacattaca
     3301 acctctcctt tagagcatta tgtgtaaggt attaagccgt gcttatgatt aagcgcctat
     3361 catatcgcct ttaaagaatg ggcgaaaaca gatatctaca ggtaaactaa gtgaaacgac
     3421 gcgtcttaat caaggagaac tcatgacgta tcaacaagct ggacgcattg ctgttttgaa
     3481 aaggattttg ggttgggtga tttttatacc tgcattgatc tctacattga tttctttgct
     3541 gaaatttatg aatacccggc aggaaaacca ggaaggcatt aatgcggtca tgctcgactt
     3601 tactcatgtc atgatcgata tgatgcaggc gaatacgcct tttttgaatc tgttctggta
     3661 taactccccg acccctaatt ttaatggtgg cgtgaacgtt atgttctggg tgattttcat
     3721 cctgattttt gtcggactgg cgttgcagga ttccggtgcc cggatgagtc gccaggcgcg
     3781 cttcttgcgg gaaggtgttg aagatcaact cattctggaa aaagccaaag gggaagaggg
     3841 gctaacgcgt gaacaaattg agtcccgtat tgttgttccg caccatacta ttttcctgca
     3901 gtttttctca ctatatatcc tgccggttat ttgtattgct gcaggttatg tgttcttttc
     3961 tctgcttggg tttatttaat caaaaggccg cacggagcgg ccattatcct caacccatat
     4021 catgctgcta ataatctatc caatgactgc tgagcaataa ccaaatgctg accgccaaat
     4081 aatcttgcac gatttaacag cgtgtagagt tggtaaaccg gttgacgttc aaggaaatcg
     4141 gcaggtagcg gggatactga ctgatagccg tcatagattt gtggcggctg ttcagtatgc
     4201 agcggtaaca tcgccaggtc gcactctcgg tcaccccagt agcaggcagg gtcgaaaatg
     4261 tacgggccat ccggacccag cgcacagttg ccggaccata aatcgccgtg caacagagaa
     4321 ggctgcggtt gatgcgaggc cagacgctgc tgaatatgct cgacgatggc gtcgatattg
     4381 ccgaaagcga tccctttctc tgctgccagt tccaactgcc agccaatccg ttgttcagca
     4441 aaaaacgttg accagcgacg ttgccaggtg ttgggctgtg gagttgtgga gagcgagtta
     4501 tcaaaatcga ggccaaattg tggttggtca ctccattgat gtaaacgcgc aatttgctga
     4561 ccaagaataa atgcgctatg cgcatccagc ggacgaggtg ggagataatc catcaccaga
     4621 aaactgtagt cacggtcagc gcctactgcc caaaccttag gcacggtgac ggttttacta
     4681 cgcgacagta actccagttg gtcggcttcg gcggtaaaac cgggaagcag ttccctttca
     4741 tcacatttga cgaaaaagtc atgtcctgca tagcgcaaat gccatgcggc atggacttct
     4801 ccgccaggca gttcattacg cagttcgatt tcgccttcac ctaactgctc gctcaaaaga
     4861 cgactgattg cctgccacat gatgtctctc ccatgttgtc tgccagatca taaggttagc
     4921 gcaagaatgc ggtgtaaaaa cacgagctaa cgcacaccac agactgatta ttgcccaaaa
     4981 accatttatt ttctgttgct attccattcc tcccaggtcg tgatggtgat ttcaggatct
     5041 ttgccggttg cactttgggt aagccccgaa accagttctc taatttcttc ttcactttgg
     5101 gtactgataa gtccaaaagt gttagtcccc agctcatgga tatttccctc atcatccgtc
     5161 atggtgagca aaaaaccgtc gcgagtcagg taattattca actcgttaat ttcagtcaat
     5221 gtatcctcat gcaacatgac ggttatgaca taacggacaa gatcgccact ggccatagtt
     5281 cacccctttg ttatcacatg acttttgagc atagtcggag aaacgcgttg ccgacaggtt
     5341 ggtggtgatt cccccaatgc tgggggaatg tttttgttag cgggaaaggt aagcgtaaat
     5401 tttttgcgta tcgtcatggg agcacagacg tgttccctga ttgagtgtgg ctgcactccc
     5461 cgcagctacg ccaaaacgga ccatctcttc aagagaggca ttttctgcca gtttcagtgt
     5521 catcgcgccg accatgctgt caccagcgcc aacggtactc tggcttttca ccggtggtgg
     5581 caccacctga atgcagtttt cactatcaac acccagcgct ccttgtggac caagggaaac
     5641 gacaacccgt ttggccttgc cgctattaac gatttcctgc gcggctttgc ggacatcgtc
     5701 tggctgggtg agttcgcgat tcaccagcgc actgagttct ttttggttag gcttaaccaa
     5761 ctcgatgtta ccaatcgcca gtgctgcact taacgcttcg ccagaactgt cgatgatgca
     5821 gcggatccct tggttttgcg cagcggaaat cagttgggtt aatttttcca gcttcacacc
     5881 tggcggcagg cttccgctta tgaccaggat ggccccggat tcaatttcca gaacttgctc
     5941 ttcaagctgg cgaaactcat cttcatttaa tgccgcgcct ggcataacaa aacgatactg
     6001 ctcaccgctt gcttccacat gtacgtgtaa attctgccgg gtccagtctt tggcttctac
     6061 agtagcgacg gggacatttt catccgccaa cagagaaacc aggtgttcgc cggtcgcgcc
     6121 acccgccggg aagatcgctg tggcactgcc tccaagatgg gcaatggcgc gggcgacgtt
     6181 gatgccgccg cccccgggtt cgaacaccgg tgcggtacag cgcagttttc cttcgggata
     6241 aatttgcggg gtaattgttg cgctatcgag agagggcgca agtgtcaacg tatagatacg
     6301 taccatcatt tcctcctata ggctgatttc agtctggcac cgaatcagcc gaaagtgaag
     6361 aaattaacaa tatgatttat tagagcttta tttaaaattt tgcagataaa tatatataaa
     6421 taaaaatctg t
//
LOCUS       DAPHDW010000197         6371 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.200, whole genome shotgun sequence.
ACCESSION   DAPHDW010000197 DAPHDW010000000
VERSION     DAPHDW010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6371
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.200"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(395..2065)
                     /gene="betA"
                     /locus_tag="RH057_004159"
     CDS             complement(395..2065)
                     /gene="betA"
                     /locus_tag="RH057_004159"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HDV3130442.1"
                     /translation="MQFDYIIIGAGSAGNVLATRLTEDPNTSVLLLEAGGPDYRFDFR
                     TQMPAALAFPLQGKRYNWAYETEPEPFMNNRRMECGRGKGLGGSSLINGMCYIRGNAL
                     DLDNWAQEPGLENWSYLDCLPYYRKAETRDVGENDYHGGDGPVSVTTSKPGVNPLFEA
                     MIEAGVQAGYPRTDDLNGYQQEGFGPMDRTVTPQGRRASTARGYLDQAKSRPNLTIRT
                     HAMTDHIIFDGKRAVGVEWLEGDSTIPTRATANKEVLLCAGAIASPQILQRSGVGNAE
                     LLAEFDIPLVHELPGVGENLQDHLEMYLQYECKEPVSLYPALQWWNQPKIGAEWLFGG
                     TGVGASNHFEAGGFIRSREEFAWPNIQYHFLPVAINYNGSNAVKEHGFQCHVGSMRSP
                     SRGHVRIKSRDPHQHPAILFNYMSHEQDWQEFRDAIRITREIMHQPALDQYRGREISP
                     GTECQTDEQLDEFVRNHAETAFHPCGTCKMGYDEMSVVDGEGRVHGLEGLRVVDASIM
                     PQIITGNLNATTIMIGEKIADMIRGQEALPRSTAGYFVANGMPVRAKK"
     gene            complement(2079..3551)
                     /gene="betB"
                     /locus_tag="RH057_004160"
     CDS             complement(2079..3551)
                     /gene="betB"
                     /locus_tag="RH057_004160"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HDV3130443.1"
                     /translation="MSRMAEQQLYIHGGYTSATSGRTFETINPANGNVLATVQAAGRE
                     DVDRAVKSAQQGQKIWAAMTAMERSRILRRAVDILRERNDELAKLETLDTGKAYSETS
                     TVDIVTGADVLEYYAGLIPALEGSQIPLRETSFVYTRREPLGVVAGIGAWNYPIQIAL
                     WKSAPALAAGNAMIFKPSEVTPLTALKLAEIYSEAGLPDGVFNVLPGVGAETGQYLTE
                     HPGIAKVSFTGGVASGKKVMANSAASSLKEVTMELGGKSPLIVFDDADLDLAADIAMM
                     ANFFSSGQVCTNGTRVFVPAKCKAAFEQKILARVERIRAGDVFDPQTNFGPLVSFPHR
                     DNVLRYIAKGKEEGARVLCGGDVLKGDGFDNGAWVAPTVFTDCSDDMTIVREEIFGPV
                     MSLLTYESEDEVIRRANDTDYGLAAGIVTADLNRAHRVIHQLEAGICWINTWGESPAE
                     MPVGGYKHSGIGRENGVMTLQSYTQVKSIQVEMAKFQSIF"
     gene            complement(3565..4152)
                     /gene="betI"
                     /locus_tag="RH057_004161"
     CDS             complement(3565..4152)
                     /gene="betI"
                     /locus_tag="RH057_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308386.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BetI"
                     /protein_id="HDV3130444.1"
                     /translation="MPKLGMQSIRRRQLIDATLEAINEVGMHDATIAQIARRAGVSTG
                     IISHYFRDKNGLLEATMRDITSQLRDAVLNRLHALPQGSAEQRLQAIVGGNFDETQVS
                     SAAMKAWLAFWASSMHQPMLYRLQQVSSRRLLSNLVSEFRRELPRQQAQEAGYGLAAL
                     IDGLWLRAALSGKPLDKPLAHSLTRHFITQHLPTD"
     gene            4281..6314
                     /gene="betT"
                     /locus_tag="RH057_004162"
     CDS             4281..6314
                     /gene="betT"
                     /locus_tag="RH057_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HDV3130445.1"
                     /translation="MTDLSHSREKDKINPVVFYTSAGLILLFSLTTILFRDFSALWIG
                     RTLDWVSKTFGWYYLLAATLYIVFVVCIACSRFGSVKLGPEQSKPEFSLLSWAAMLFA
                     AGIGIDLMFFSVAEPVTQYMQPPEGAGQTIEAARQAMVWTLFHYGLTGWSMYALMGMA
                     LGYFSYRYNLPLTIRSALYPIFGKRINGPIGHSVDIAAVIGTIFGIATTLGIGVVQLN
                     YGLSVLFDIPDSMAAKAALISLSVIIATISVTSGVDKGIRVLSELNVALALGLILFVL
                     FMGDTSFLLNALVLNVGDYVNRFMGMTLNSFAFDRPVEWMNNWTLFFWAWWVAWSPFV
                     GLFLARISRGRTIRQFVLGTLIIPFTFTLLWLSVFGNSALYEIIHGGAAFAEEAMVHP
                     ERGFYSLLAQYPAFTFSASVATITGLLFYVTSADSGALVLGNFTSQLKDINSDAPGWL
                     RVFWSVAIGLLTLGMLMTNGISALQNTTVIMGLPFSFVIFFVMAGLYKSLKVEDYRRE
                     SANRDTAPRPLGLQDRLSWKKRLSRLMNYPGTRYTKQMMETVCYPAMEEVAQELRLRG
                     AYVELKSLPPEEGQQLGHLDLLVHMGEEQNFVYQIWPQQYSVPGFTYRARSGKSTYYR
                     LETFLLEGSQGNDLMDYSKEQVITDILDQYERHLNFIHLHREAPGHSVMFPDA"
BASE COUNT         1299 a         1754 c         1763 g         1555 t
ORIGIN      
        1 tgatgattaa ttaaagaaga gtaaaaataa atcactgcca ataaattata cgaatcgtgt
       61 ttaaagctcc acgttgtttt ggtctgactt atttttgtcc tcgtttcaag aacccttttc
      121 gtatatgcta accgtgagcg ggagtgaaca agtcaccata gccaggaaca ctagcggcgt
      181 aattcttaag ctgagtggca tttcacctgc ggtttatcag tgcattcata gcaccttcat
      241 cagccagatt gaacgagtcc gcttagaatt tgctggcgag gttgtcacta tgaagcatga
      301 gagttacccc tatgttttat ataacgattc tgtccccata cctggaacgg taactctcaa
      361 tcagatcggt tcttgagtca gttcaaatca cgactcattt tttcgctctc accggcatcc
      421 catttgccac aaaatatccc gccgtgctcc tcggcagcgc ttcctgtcca cgaatcatat
      481 ccgctatttt ctcgccaatc ataattgtcg tggcgttcaa attcccggtg ataatctgcg
      541 gcataatcga cgcatccacc acacgcaggc cttctaaccc gtgtacgcgg ccttcgccgt
      601 caaccacgga catctcgtcg taacccattt tgcaggtacc gcacggatgg aaggcggttt
      661 cggcgtggtt acgcacgaac tcatcgagct gttcatccgt ctgacattcc gtgccggggc
      721 tgatttcgcg gccacgatac tgatccagcg cgggttgatg catgatctcg cgggtgatgc
      781 gaattgcgtc gcggaactcc tgccagtcct gctcgtgcga catgtagtta aacagaatcg
      841 ccggatgctg gtgcgggtcg cgggatttaa tccgcacatg cccacggctt ggcgagcgca
      901 ttgagccgac gtggcactgg aaaccgtgct ctttcactgc attcgagccg ttatagttaa
      961 tcgctactgg caggaaatgg tactgaatat tcggccacgc aaattcctca cggctgcgaa
     1021 taaatccacc tgcttcaaag tggttgctgg caccaacgcc agtgccgcca aacagccact
     1081 ccgcaccgat tttcggttgg ttccaccact gcagggcagg gtagagggaa accggttctt
     1141 tgcactcata ttgcagatac atctccagat gatcctgaag attttcgccg acgccgggta
     1201 attcatgcac cagcggaata tcaaactccg ccagcagttc agcgttgccg acgccggagc
     1261 gttgcaggat ctgcggtgag gcaatcgcgc ctgcacataa cagcacttct ttgttggccg
     1321 ttgcgcgggt tgggatggtg ctgtcgcctt ccagccattc gacgcccacc gcgcgtttgc
     1381 cgtcaaaaat gatgtgatcg gtcatagcgt gagtacgaat ggtcaggtta gggcgcgatt
     1441 tggcctgatc gagataaccg cgcgcggtac tggcgcgacg gccctgcggc gtgacggtgc
     1501 gatccatcgg gccaaaacct tcctgctgat agccgttgag atcgtccgtg cgcgggtagc
     1561 ctgcctgcac gcccgcttca atcatcgctt caaacagcgg attgacgccg ggtttggagg
     1621 tggtgacgct caccgggcca tcgccaccgt ggtagtcgtt ctcgcccaca tcgcgagtct
     1681 cggccttgcg gtagtagggc aggcagtcga gataactcca gttctccaga ccgggttctt
     1741 gcgcccagtt atcgagatcc agcgcattgc cacggatgta gcacatgccg ttgatcagcg
     1801 acgatccacc caggccttta ccgcgtccgc actccatgcg gcggttattc ataaacggtt
     1861 caggctctgt ttcataggcc cagttgtagc gtttgccctg tagtgggaac gccagcgctg
     1921 cgggcatctg ggtgcggaag tcaaagcgat agtccgggcc gcccgcttca agcagcagca
     1981 cggaggtatt cggatcttca gtcagacggg tagcgagaac gttgccggct gagccggcac
     2041 caataatgat gtagtcaaat tgcaaataaa cctcctggtt agaatatgga ctggaattta
     2101 gccatctcaa cctggatgga cttcacctgg gtgtaactct ggagcgtcat cacgccgttc
     2161 tcgcgaccaa tgccggagtg tttgtagccg ccaacgggca tctctgccgg ggattcgccc
     2221 caggtgttga tccagcaaat acccgcttcc agctgatgaa tgacgcgatg cgcgcggttc
     2281 aggtccgccg tcacgatgcc cgccgccagg ccgtagtcgg tatcattggc gcggcgaatc
     2341 acttcgtcct cagattcata agtgagaagc gacatcactg gcccgaagat ctcttcacgc
     2401 acgatggtca tatcgtcgct gcaatcggtg aacactgtcg gtgcaaccca tgcgccgtta
     2461 tcgaagccat cgcctttcag tacatcgccg ccgcacagta cgcgcgcgcc ttcctctttg
     2521 cctttggcga tatagcgcag cacgttatcg cgatgcggga agctgaccag cgggccgaag
     2581 ttagtttgcg gatcgaaaac gtcgcccgcg cgaatgcgct caacgcgcgc cagaattttc
     2641 tgctcaaatg cggctttgca tttcgccgga acgaagacgc gggtgccatt ggtacacacc
     2701 tgaccggagc tgaagaagtt tgccatcatg gcgatatcgg cggcgagatc gagatccgca
     2761 tcatcgaaaa cgatcagcgg tgatttaccg cccagttcca tggtcacttc tttcagggaa
     2821 gaggccgccg agttagccat cacttttttg ccgctggcga caccgccggt aaatgacact
     2881 ttggcaatgc ccggatgctc ggtcagatat tgcccggtct ccgcgcccac gcccggcaac
     2941 acgttaaata cgccgtccgg caggcccgct tcgctgtaaa tttcagccag ctttaacgcg
     3001 gtaagcgggg taacttcgct cggtttgaaa atcattgcgt tgcctgccgc cagcgccggg
     3061 gcggatttcc acagggcaat ctggatcggg tagttccatg cgccaatccc tgccactacg
     3121 cccagcggtt cgcggcgggt atacacaaag gacgtttcac gcaacgggat ctggctgcct
     3181 tccagcgccg ggatcagccc ggcgtagtac tccagcacgt ccgcaccggt aacgatatcg
     3241 acggttgagg tttccgaata tgcttttccg gtgtcgaggg tttccagttt tgcgagttcg
     3301 tcattgcgtt cacgcagaat atcaacggcc cgacgcagaa tacgcgagcg ctccatagcg
     3361 gtcatcgccg cccagatttt ttgcccctgt tgggcactct tcacggcgcg atcgacatcc
     3421 tcacgcccgg cggcctgcac ggtcgccagc acgttaccgt tggccgggtt aatggtctcg
     3481 aaggtgcgac cgctggtggc ggaggtataa ccaccatgta tataaagctg ctgttctgcc
     3541 attcgggaca tcacgtctcc tcggttaatc ggtgggtaaa tgctgagtaa taaagtgacg
     3601 ggtcagcgag tgtgcgagag gtttatccag cggtttgccg ctcagtgccg cgcgcagcca
     3661 taatccgtca atcagcgcgg caaggccgta gcccgcttcc tgtgcctgtt ggcgcggtaa
     3721 ctcgcggcga aattcgctca ccagattcga caataagcgg cggctactga cctgctgtaa
     3781 acgatagagc atcggctgat gcatactgct ggcccagaac gccagccagg ctttcatcgc
     3841 cgcactgctc acctgcgttt catcgaagtt tccgccaaca atcgcctgta atcgctgctc
     3901 tgcactgccc tgcggaagtg catgtaatcg attcaaaacc gcgtcacgca gctgactggt
     3961 gatatcgcgc atggttgctt ccagcagacc atttttgtcc ctgaaatagt ggctgatgat
     4021 ccccgtagaa acgcctgcac ggcgggcgat ctgcgcgatc gttgcatcgt gcatgcccac
     4081 ttcatttatt gcttccagtg tggcgtcgat cagttgtctg cgccggatcg actgcatccc
     4141 caatttgggc attttcgcca ctccattcat cagcgttgtt tatctattaa agcggttatt
     4201 gattggacgt tcaatataaa atgtgtctta attgttacga atttgatttt aaatggtaac
     4261 aataacagtg gggatactgg atgacagacc tttcacacag cagggaaaag gacaaaatca
     4321 atccggtggt gttttacacc tccgccggac tgattttgtt gttttccctg acaacgatcc
     4381 tgtttcgcga cttctcggcc ctgtggattg gccgcacgct ggactgggtt tctaaaacct
     4441 tcggttggta ctatctactg gcggcaacgc tctatattgt ctttgtggtc tgtatcgctt
     4501 gttcgcgttt tggttcggtg aagctcgggc cagaacaatc caaaccggaa ttcagcctgc
     4561 tgagttgggc ggcgatgctg tttgctgccg ggatcggtat cgacctgatg ttcttctccg
     4621 tagccgaacc ggtaacgcag tatatgcagc cgccggaagg cgcgggacag acgattgagg
     4681 ccgcgcgtca ggcgatggtc tggacgctgt ttcactacgg cttaaccggc tggtcgatgt
     4741 atgcgctgat gggcatggcg ctcggatact ttagctatcg ttataatttg ccgctcacca
     4801 tccgctcggc gctgtacccg atcttcggta aacggattaa cgggccgata gggcactcag
     4861 tggatattgc agcggtgatc ggcaccatct tcggtattgc cactacgctc ggtatcggtg
     4921 tggtgcagct taactatggc ttgagcgtac tgtttgatat tcccgattcg atggcggcaa
     4981 aagcggcact gatctccttg tcggtgataa tcgccacgat ctctgtcacc tccggtgtcg
     5041 ataaggggat tcgtgtgtta tcggaactta acgttgcgct ggcgctggga ttgatcctgt
     5101 tcgtattgtt tatgggcgac acctcgttcc tgcttaatgc gctggtgctg aatgttggcg
     5161 actatgtgaa tcgctttatg ggcatgacgc tcaacagttt tgccttcgac cgtccagttg
     5221 agtggatgaa taactggacg ctcttcttct gggcatggtg ggtggcatgg tcgccgtttg
     5281 tcggcttgtt cctggcgcgt atctcgcgtg ggcgtaccat tcgccagttc gtgctgggca
     5341 cgttgattat tccgtttacc ttcacgctgt tatggctctc ggtgttcggc aatagcgcgc
     5401 tgtatgaaat catccacggc ggcgcggcat ttgccgagga agcgatggtc catccggagc
     5461 gcggcttcta cagcctgctg gcgcagtatc cggcgtttac ctttagcgcc tccgtcgcca
     5521 ccattactgg cctgctgttt tatgtgacct cggcggactc cggtgcgctg gtgctgggga
     5581 atttcacctc gcagcttaaa gatatcaaca gcgacgcccc cggctggctg cgcgtcttct
     5641 ggtcggtggc gattggcctg ctgacgctcg gcatgctgat gaccaacggg atatccgcgc
     5701 tgcaaaacac cacggtgatt atggggctgc cgttcagctt tgtgatcttc ttcgtgatgg
     5761 cggggttgta taaatctctg aaggtagaag attaccgccg tgaaagtgcc aaccgcgata
     5821 ccgcgccgcg accgctgggg cttcaggatc gcctgagctg gaaaaaacgt ctctcgcgcc
     5881 tgatgaatta tccgggcacg cgttacacta aacagatgat ggagacggtc tgttacccgg
     5941 caatggaaga agtggcgcag gagctgcggt tgcgcggcgc gtacgtggag ctaaaaagcc
     6001 tgccgccgga agagggacaa cagttggggc atctggattt gttggtgcat atgggcgaag
     6061 aacaaaactt tgtctatcag atttggccgc agcaatattc ggtgccgggc tttacctacc
     6121 gcgcacgtag cggtaaatcg acctactacc ggctggaaac cttcctgtta gaaggcagcc
     6181 agggcaacga cctgatggac tacagcaaag agcaggtgat caccgatatt cttgaccagt
     6241 acgagcggca ccttaacttt attcatctcc atcgtgaagc gccgggccat agcgtgatgt
     6301 tcccggacgc gtgattgttt ttgcataaaa ccgcgccatg tctgcatatg gcgcgggaat
     6361 cattgactcg c
//
LOCUS       DAPHDW010000198         6202 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.201, whole genome shotgun sequence.
ACCESSION   DAPHDW010000198 DAPHDW010000000
VERSION     DAPHDW010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6202
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.201"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..419)
                     /locus_tag="RH057_004163"
     CDS             complement(<1..419)
                     /locus_tag="RH057_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tar ligand binding domain-containing protein"
                     /protein_id="HDV3130446.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNIDEKYKNYYTALTELID"
     gene            complement(564..1067)
                     /gene="cheW"
                     /locus_tag="RH057_004164"
     CDS             complement(564..1067)
                     /gene="cheW"
                     /locus_tag="RH057_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HDV3130447.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(1088..3052)
                     /gene="cheA"
                     /locus_tag="RH057_004165"
     CDS             complement(1088..3052)
                     /gene="cheA"
                     /locus_tag="RH057_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HDV3130448.1"
                     /translation="MSMDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASFDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQSPR
                     RIILSRLKAGEVDLLEEELGHLTTLTDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKISTPPVLKLAAEQAPTGRVEREKTTRSNESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(3057..3983)
                     /gene="motB"
                     /locus_tag="RH057_004166"
     CDS             complement(3057..3983)
                     /gene="motB"
                     /locus_tag="RH057_004166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HDV3130449.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRPHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(3980..4867)
                     /gene="motA"
                     /locus_tag="RH057_004167"
     CDS             complement(3980..4867)
                     /gene="motA"
                     /locus_tag="RH057_004167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV3130450.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGVVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(4994..5572)
                     /gene="flhC"
                     /locus_tag="RH057_004168"
     CDS             complement(4994..5572)
                     /gene="flhC"
                     /locus_tag="RH057_004168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HDV3130451.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(5575..5925)
                     /gene="flhD"
                     /locus_tag="RH057_004169"
     CDS             complement(5575..5925)
                     /gene="flhD"
                     /locus_tag="RH057_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HDV3130452.1"
                     /translation="MHTSELLKHIYDINLSYLLLAQRLIVQDKASAMFRLGINEEMAT
                     TLAALTLPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGIMLSTRLLN
                     DVNQPEEALRKKRA"
BASE COUNT         1476 a         1650 c         1491 g         1585 t
ORIGIN      
        1 taatcaatca gttcagttaa cgctgtgtaa tagtttttat atttttcatc aatattacga
       61 ctggtagcga ccatttcagg taacggtgcc atgcttttga attttttata atgcgtcgct
      121 gcctgcgcca atgttttcct ggcgctatcg agcaattcaa ctttggcgtt actttgttga
      181 ttagaggaat ccatcatcat ccgcaccgct gaacgactca ggttaatgcg cgtttgcagc
      241 attaaatccc aggttgacgt cagctcgccc tgctgttccc gcaattgatt ggaaaccaca
      301 aagctcttct ggctatggtg aagggaagaa aaaaacagac tgccggaaat aagctgtaac
      361 agtgcgaata cccccagcac cattaccaac agcgtgacta cgcggatacg gttaatcata
      421 aggcaccttc ctgaaaacaa gttgatctcg ttatcggcaa gaagggggga aactttattg
      481 ctgatgccac ccgccgcgaa attgaaataa aaaacccgat gcgcagatca tcgggttcat
      541 ttcaattgag gaaatcggga gaattacgcc acttctgacg ccgcgctatc taacagcgcc
      601 atctcttcgc tgttcagcag tttttcgatg ttcaccagaa tcaacatacg gtcgcccagt
      661 gcgcccagtc cagtgagata ttctgttgaa agcgtcacgg caaactctgg tgccggacga
      721 atttgctccg ccgtcaatga aagcacgtct gaaacgccgt caaccacgat gccgaccacc
      781 cgctgtccga gattcaggac gataactacc gtgttgtcgt tatagtccac atccacctgg
      841 ctgaacttaa ttcgtaagtc aacaatcggc acaataacgc cgcgcagatt cgtgacgcct
      901 ttgataaacg ctggcgtgtt cgcaatccgt gttacctgat cgtagccacg gatctcctgc
      961 actttcagga tatcaatacc gtactcttca tcaccaaggg taaataccag aaattcctgg
     1021 cctgacggct cgctggccag ctttgttaca ttcgtcatac cggtcatatt gttacctttt
     1081 tactcattca ggcggcggtg ttcgccatac gttgttcgcg gtttatcgcc tgcaaggcgg
     1141 agacatcaac aatcagtgcc acgctgccgt cgccaagaat ggtcgcagca gaaatgccgg
     1201 ggactttgcg atagttactt tcaaggtttt taaccacaac ctggtgttga ccaattaatt
     1261 gatccaccag caaggcgtag cggcgaccgc cactttgtaa gatcacaaca attccctggg
     1321 tggcttcggt tttcgcgccc gcgacgttga acactttcca cagttcgacg atgggcagat
     1381 attcaccccg cacttccagc acccgctcgc cgccggccag tggatgcaga tcggcttcac
     1441 ggggttgcag tgattccatg acagcattca gcggcagaat gaaaacttca tccgcaacgc
     1501 gtacggacat gccgtcgagg atggccaacg tcagcggcag taaaatgcgg atcgtagtgc
     1561 cagtaccctg cttcgactgg atttcgacat gaccgcccat ctcctggata ttacgtttaa
     1621 cgacgtccat gccgacgccg cgcccggaga cgtcggtgac ctgctccgcc gtggagaagc
     1681 caggtgcaaa tatcagcatt gcaacttcgt cgtcgctcat gttttcgctg acagtcaaac
     1741 cttgcgaggc cgcttttgcc agaattcgct cacggtttag ccccgcccca tcgtcggtca
     1801 cttcaatgca aatgttgccg ccctgatgtt cggcagacag aattaaattt ccgacgctgt
     1861 ttttacctgc ggcgagccgt ttttctggca gttcaatacc gtgatcgagg ctattgcgta
     1921 ccaggtgggt cagcgggtcg ataatgcgtt ctatcaggct tttgtcgagt tcagtagaac
     1981 tgcccaccag cgtcagttct acctgcttgc cgagttttcc cgccagatca cgcaccagcc
     2041 ggggatagcg actaaaaaca tattccatcg gcatcatgcg aatcgacatc actgattcct
     2101 gcaaatcacg ggcgttacgt tgtaactgcc ccatgctggt tatcaaatca ccatgattaa
     2161 ccgggtccag ttcgctggaa cgctgggcaa gcatggactg ggtgataacc agctcgccga
     2221 cgaggttaat taattgatca accttttcta ccgctacacg gatgctggtg gattcattgc
     2281 tgcgcgtcgt tttttcccgc tccacgcggc cggttggcgc ttgttcggct gccagtttaa
     2341 gcactggtgg ggtggatatt tttggcgaga cttctactgt ttcaaaagta atctgatcgg
     2401 cttcaatcac aaaacagagt accgctgtga tgtcatcttc ggcaatgtcg cccggtaata
     2461 ttgccgagag cgaatccgcc cctttcacca cgtcagttaa cgttgtcaga tgtcccagtt
     2521 cttcttccag caggtcgact tccccggcct tcaggcgcga aaggataatt cgtcgcggcg
     2581 actgactgcg actctgctca tcttgcggtt cacttttggc aaccacactt aatcgggtca
     2641 ctgcggatgg cgtttcgcct ttcgcttcta atgccagttg acgcaaggcc tggcagatat
     2701 aatcgaagct ggcggcatcc ggctcttgcg actgtttata agcgtcgagc tgttcttgca
     2761 tgatgtcctt cgtttccaaa aacagattga taatgtcggt gttgagttgc atctcacctc
     2821 gtctggcttc atcgagcagg ttttccatca gatgcgtggt ttcctgcaaa acgctgaagc
     2881 caaaagttcc tgcccctcct ttgatcgagt gggcagcccg aaagatggca ttcaattgtt
     2941 cggcatctgg cgcttccggc tgcaaaacca gcagatgctg ctccatgtca gccaacagtt
     3001 cgtccgcttc atcaaaaaat gtctgataaa aatcgcttat atccatgctc acgctgtcac
     3061 ctcggttcgg ctgatggcat tgtgggaaca ctgacctgtg gtgcaacctc aggtttttcc
     3121 agggcgctta ctggctcatt ctggctttcg gcgttttcat gcaaaatggc ctgttcggct
     3181 tgtttgttca gtaccagcag gctgatgcga cggttgacgg catcatcagg tccgcgatcg
     3241 cttaagcgca tcgttgccgc catgccgaca acacgtaaca ctttgccgct atccaacccg
     3301 ccgaccatca gttcgcggcg ggatgcattg gcccgatcgg cagaaagctc ccagttgctg
     3361 taacctttct caccgctggc gtagggaaaa tcatcggtat gacctgaaag gctaatacgg
     3421 ttgggaatac cgttcagtac aggcgcgatg gcgcgcagaa tgtcgcgcat atagggttcg
     3481 acatcggcac tgccggttct aaacatcggg cgattctggc tatcgatgat ctgaatacgt
     3541 agaccttcct ggaccagatc gattttgaga tgtggacgca acgcccgcag tttcggatcg
     3601 gactctatca actggtcgag atcaccccgc aatttccgca atcgactttg ctccatgcgt
     3661 tttttcaact cttcgatgtt cggctgctta ttcacttccc cctggctttg ggtgtaatca
     3721 tcaccaccgc cgggaattgg gctttcacta ttagaaatgc gatcgccgcc cgtaaccgca
     3781 gtcgccagtg gagtccggaa gtactccgca atctgaatca gctcttttgg gctggagatg
     3841 gagatcagcc acatcaccag aaaaaaggcc atcatcgcag tcataaagtc ggcataagca
     3901 atcttccacg atccatgtgc tgccccgtgg cttttggctt tgcgtcgttt gacgacaata
     3961 atcggatgcg cttgattctt catgcttcct cggttgtcgt ctgttgttgc ggatttttca
     4021 ccgcacgcac atgctcttcc agttcaataa acgacggacg ttcgctggaa tagagcgttt
     4081 tgcgaccaaa ctcaacggcg ataggcggtg cgtaaccgtt cagattagaa agcagagtga
     4141 ctttgacgca ctgcatcatt ttgctggttt cggcgctttt ctgacgtaaa acactcgcta
     4201 atggggaaat aaatccgtaa gccaataaaa tgccgaggaa agtccccacc atcgcatgtg
     4261 cgataagcgc acccagctcg gcggcaggac gatcggctga acctaacgcg tgaacgaccc
     4321 ccattacagc cgcgacaata ccaaacgccg gaagtgagtc cccgaccagc gccagactgt
     4381 tcgccgggac ttctgcctcg ctttcgtgcg tctcaatctc ttcatccatc agggcttcga
     4441 tttcgaaggt gttcatatga ccgctgataa tcagacgcag ataatcgacg ataaaatcaa
     4501 gcatgacgct atccgcgagg atgcgtgggt agctggcgaa gatctcgctc tcacggggat
     4561 tttcaatatc acgttccagc gaaaacatcc ccatctgccg cgatttcgcc atcaaccgat
     4621 aaagcagagc cagcagatcc atatacattg ctttggtgta tttggagcga cgaaacagca
     4681 acggcagcgc cttcagcgtg cctttaatcg ctttgccatt attgccgacg ataaacgacc
     4741 caatccctgc accggcaata atcaccagtt cagcgggttg atagagtgct ccaaggcttc
     4801 caccggtcat caaataaccg ccgaaaactg taccgagaac aaccaggtaa cctaataaga
     4861 taagcacgac atcatccttc cactgttgac catgacagga tgttcagtcg tcaggcgtta
     4921 acgcgcgatt ggggcaaaaa aaagcagcgg tacgtcgtta ccgctgctgg aatgttgcgc
     4981 ctcaccgtat cagttaaaca gcctgtactc tctgttcatc cagcagttgt gggataatat
     5041 cggcaggatt ctgggaaagt ttacgtcttt ttactgcccg ggatggcggt tgacataagc
     5101 tgcaggcaaa gctgccaaca ggctggtgag cgtgggtaat aaaattgccg ccgcagcagt
     5161 tgcagctgga aagttgcagt aatccacttt caacaaaccg caccaatgtc caggcacggg
     5221 ttaatgccag cagtggtcct tcttctgctt gtgggcactg ttcaaggtat aaacggtagg
     5281 ctttgatcac cgcatcgacg ccattacaca aaccggtttt cagtaaaaac tgccatgcat
     5341 tacagaacat cgaagcatga acgttttgtt cccaggtcat aaaccagtcg gttgagaatg
     5401 gcagcatgcc tttcggcggt gggcttccgc gcagttcttt ataaagtttt atcaggcgtc
     5461 cgcgacttaa ctgtgtttcg ctttccagca tctgcaaacg agcgcccagg gtgatcaatt
     5521 ccattgccag ctgaatatcc cgcgcttcct gaacaatgct tttttcactc atgatcaggc
     5581 ccttttcttg cgcagcgctt cttcaggctg attaacatca ttcagcaagc gtgttgagag
     5641 catgatgccg gtatgaattt gctggagatc gtcaacgcgg gaatcttgcg tcaactgagt
     5701 aatcgtctgg tggctgtcaa aacggaagtg acaaaccagt tgattggttt ctgccagctt
     5761 aaccatttgc ggaagagtca gtgccgctaa cgttgtcgcc atttcttcat ttatgccgag
     5821 acgaaacata gcggacgctt tgtcctgaac aatcaaacgc tgtgcaagta gtaaatatga
     5881 caagttgatg tcataaatgt gtttcagcaa ctcggaggta tgcattattc ccacccagaa
     5941 taaccaactt tatttttatg cggtttcacc gcaccccgtg atgtcgccgg gaagccccgg
     6001 taaaaaataa ttagcattag aatagttgcg ataagctgca ataagcagaa ccaccttttt
     6061 ggtttaatat gtccttacaa atagaaatgg gtctttacac ttatctaaga tttttcctaa
     6121 atcgacgcaa ctgtactcgt cactacacgc acatacaacg gaggggggct gcgattttca
     6181 ataatgtgtg atgcagatca ca
//
LOCUS       DAPHDW010000199         6190 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.202, whole genome shotgun sequence.
ACCESSION   DAPHDW010000199 DAPHDW010000000
VERSION     DAPHDW010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.202"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            124..900
                     /gene="exuR"
                     /locus_tag="RH057_004170"
     CDS             124..900
                     /gene="exuR"
                     /locus_tag="RH057_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ExuR"
                     /protein_id="HDV3130453.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGPFELLQARQ
                     LIESNIAEFAATQVTKQDIMKLMAIQEQARGEQCFRDSEWDLQFHIQVALATQNSALA
                     AIVEKMWTQRSHNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHL
                     ENTKIMLFNETSDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            1245..1907
                     /locus_tag="RH057_004171"
     CDS             1245..1907
                     /locus_tag="RH057_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HDV3130454.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1911..2294
                     /gene="mzrA"
                     /locus_tag="RH057_004172"
     CDS             1911..2294
                     /gene="mzrA"
                     /locus_tag="RH057_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HDV3130455.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            2441..2809
                     /locus_tag="RH057_004173"
     CDS             2441..2809
                     /locus_tag="RH057_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
                     /protein_id="HDV3130456.1"
                     /translation="MKYRIALAVSLFALSAGSYATTLCQEKEQNILKEISYAEKHQNQ
                     NRIDGLNKALSEVRANCSDSQLRADHHKKIAKQKDEVAERQQDLAEAKQKGDADKIAK
                     RERKLAEAQEELKKLEARDY"
     gene            2847..3152
                     /locus_tag="RH057_004174"
     CDS             2847..3152
                     /locus_tag="RH057_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HDV3130457.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            3155..3559
                     /locus_tag="RH057_004175"
     CDS             3155..3559
                     /locus_tag="RH057_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HDV3130458.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3549..3848
                     /locus_tag="RH057_004176"
     CDS             3549..3848
                     /locus_tag="RH057_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HDV3130459.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDLSASRREWLEATGAYDRRWN
                     MLLSLRSWALVGSSVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            4034..4426
                     /locus_tag="RH057_004177"
     CDS             4034..4426
                     /locus_tag="RH057_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HDV3130460.1"
                     /translation="MKKLEDVGVLVARILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLPLVILLEFGGGLAILFGFLTRTTALFTAGFTLLTAFLFHSNFAEGVNSLMFMKN
                     LTISGGFLLLAITGPGAYSIDRLLNKKW"
     gene            4496..5482
                     /gene="yqjG"
                     /locus_tag="RH057_004178"
     CDS             4496..5482
                     /gene="yqjG"
                     /locus_tag="RH057_004178"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HDV3130461.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5775..6140
                     /locus_tag="RH057_004179"
     CDS             5775..6140
                     /locus_tag="RH057_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HDV3130462.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTTIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEP"
BASE COUNT         1557 a         1525 c         1549 g         1559 t
ORIGIN      
        1 ataaatattc acaatattaa ttcaatacat taattcaatc accaaattta tatatgacca
       61 atggcatacc catgaataaa gtggtatgat aacttcgtcc gcattccact ccgggagacg
      121 catatggaaa tcactgaacc acgccgtttg tatcaacaac ttgccgctga cctgaaagag
      181 cgcatcgaac agggcgtcta tctggtaggt gataaactgc ctgcagaacg ctttattgcc
      241 gatgaaaaga acgtcagccg tacggtagtt cgtgaagcca tcatcatgct ggaagttgaa
      301 ggctatgtcg aagtgcgtaa aggttcaggt attcatgtgg tttccaacca gccgcgccat
      361 cagcaggcag ctgacaataa tatggaattc gccaattacg gtccgtttga gttgcttcag
      421 gctcgccagc tcatcgaaag taatattgcc gagttcgcgg caactcaggt aacgaaacaa
      481 gacatcatga aactgatggc cattcaggaa caggcgcgcg gcgaacaatg cttccgtgat
      541 tccgagtggg atttacagtt ccacattcag gtagccctgg cgacgcagaa ctccgccctg
      601 gcggccatcg ttgaaaaaat gtggacccaa cgtagccata acccgtactg gaaaaaactg
      661 cacgaacaca ttgattcccg taccgtcgat aactggtgtg atgaccacga tcaaatcctc
      721 aaggcgctga ttcgcaaaga tcctcatgct gctaagctgg caatgtggca gcacctggaa
      781 aacaccaaga tcatgttatt caacgaaacc agcgacgact tcgagttcaa tgccgaccgc
      841 tatctattcg ccgaaaaccc ggtggtgcat ctcgatacgg ccaccagtgg cagtaaatga
      901 aatacctgtc cgacaggcgc tgcgacgcgc ctgtttgtct cgtcggtaag cgaaaagtat
      961 aaagtgtcag cctgtgtaaa tcctctcgcc accctcccct gcattcagca aaatcagact
     1021 ccacggacat gtaattttga taacgaacaa cgttgacctt tgttacaatt agattcaatt
     1081 tgaatttatg tttttgaatg ctttcttatc tcacgattta acagggaata gttcaggctg
     1141 tgttgatgta tcaaacccgc agaacatacc aaaacagcaa taacattgcg gtagtgcatc
     1201 ttttaaaacc agcgtggcgt taaccgattc accaggaata atgaatggaa cttttgaccc
     1261 aattgctgca agccctgtgg gcgcaggatt ttgaaaccct ggccaatcca tcgatgattg
     1321 gcatgttgta ttttgtcttg tttgtaattt tgttccttga aaacggcttg cttccagcgg
     1381 cctttttacc gggcgacagt ttactggtat tggtcggcgt gttgattgcg aaaggcgcga
     1441 tgggttaccc gcaaacgatt ctgctgctga ccgttgccgc cagcctcggc tgctgggtca
     1501 gctatattca ggggcgatgg ctgggcaata cccgcaccgt acaaaactgg ctatctcatt
     1561 tacccgcgca ttatcatcaa cgcgcacacc atctttttca taaacacggt ttatcggcgc
     1621 tgttaattgg tcgctttatt gcgtttgtca gaacactgct gccgacgatt gccgggttat
     1681 cagggctgaa taacgcgcgc tttcagtttt tcaactggat gagcggtctg ctgtgggtat
     1741 tgatcctgac aactctgggt tacatgctcg gcaaaacgcc ggtattttta aagtacgagg
     1801 accagctgat gtcatgcctg atgctgctcc cggtggtgct gctggtgttt ggcctggcag
     1861 gttctctggt cgtgttatgg aaaaagaaat atggaaatcg ggggtaaggg atgcaaatac
     1921 ctcgcatgtc gcttcgccag ctagcctggt ccggcactgt tttacttctg gtcggcacgc
     1981 tgttgctggc ctggtccgcg gttcgccagc aagagtctac gctggcgatt cgtgccgttc
     2041 atcaaggcac aacgatgcca gacggttttt cgatctggca tcaccttgac gctcatggca
     2101 ttcctttcaa aagtatcacc cccaaaaacg acactctgtt aattacattt gactccagcg
     2161 accagagcgc cgccgcaaaa gcggtcctcg acagaacatt gccccatggc tacatcattg
     2221 cgcagcagga caataacagt caggctatgc agtggctgac ccggttacgg gataactctc
     2281 atcgcttcgg ataacttcct gttatccgaa acatatcact cactttggtg atttcaccgt
     2341 aactgtctat gattaatgag gcggtggtta acactaccgc ttcgttgtct ggacaatcgt
     2401 tcctttgtaa taggtccgaa tcacaatgga aggttcaaga atgaaatacc gcatcgcttt
     2461 agctgtttct ctctttgctc ttagtgccgg tagttatgcc actaccctgt gtcaggaaaa
     2521 ggagcaaaat atccttaagg agatcagcta tgccgaaaaa caccaaaacc agaatcgtat
     2581 tgacggtctg aataaagccc tgagtgaagt ccgggccaac tgttcagata gccagctgcg
     2641 tgccgatcat cacaagaaaa tcgcaaagca gaaagatgag gtggcggaac gccagcaaga
     2701 tttagccgag gcgaagcaaa aaggcgatgc cgataagatt gccaaacgcg aacggaaact
     2761 ggcagaagcg caggaagagc tgaaaaagct ggaagcgcgc gactactaac tcacaatagt
     2821 cactacttac tcacctggag aaaactatgt cgaaagaaca cactacggaa catctgcgtg
     2881 ctgagttgaa atccctttcc gatacgctgg aagaggtgct tagctcatct ggcgagaagt
     2941 cgaaagaaga gttgagtaag attcgtagca aagcggagca ggcactgaaa cagagccgtt
     3001 atcgcctggg tgaaaccggt gatgccatcg ccaaacaaac ccgtgtcgcg gcggcgcgtg
     3061 ctgatgagta tgtgcgtgaa aatccgtgga ccggcgtggg aattggcgct gcaatcggtg
     3121 tagtgctcgg cgttctgctg tcgcgtcgtt aattatggcg gacactcatc acgcgcaagg
     3181 gcccggtaaa agcgttctgg gcatcgggca gcgaattgtt tccatcatgg ttgaaatggt
     3241 ggaaacacgt ctgcggctgg cggtggtgga gctggaagag gaaaaagcta atctctttca
     3301 acttttactg atgctgggcc tgacgatgct tttcgctgca tttggtctta tgagcctgat
     3361 ggtgctaatt atttgggcgg ttgatccgca atatcgcctg aatgcgatga ttgccaccac
     3421 cgtggtgttg ctgctactgg cactgattgg cggtatctgg acgttacgta aatcgcgtaa
     3481 atccaccttg ctgcgccata cacgccatga gctggcaaac gatcggcaac tgctcgagga
     3541 ggagtcccgt gagcagtaaa gtcgaacgtg aacgacgtaa ggcgcaactg cttagccaga
     3601 tccagcaaca acggctggat ctttccgcca gtcgtcgtga atggctggag gcaacaggcg
     3661 cttacgatcg tcgctggaat atgctgctaa gtctgcgctc ctgggcgctg gttggcagta
     3721 gcgtgatggc gatctggacg attcgccatc ctaatatgct ggtccgctgg gccagacgcg
     3781 gttttggcgt atggagcgcc tggcgtctgg ttaaaacgac cctcaagcag caacagcttc
     3841 gcggttaact tccctctggc cggagcaatt ccggccttat ccctcaaatt ttttgaagat
     3901 ttttgacagt tttccttgct aacaatcatc attcaccacg tttatgattc tctccatcga
     3961 cagcaacgac gctaataccg cgcccttgca caaaaaaaca atcagcagcc tgagtggcgc
     4021 agtggagagt atgatgaaaa aattagaaga tgttggtgta ctggtagcgc gcattttaat
     4081 gccgattctg tttattaccg caggctgggg aaaaatcacc gcttactctg gtacccaaca
     4141 atatatggaa gcaatgggtg tcccgggttt tatgctgccg ctggtgattc tgcttgagtt
     4201 tggtggtggt ctggcaatcc tgttcggttt cctgactcgc accacagccc tgtttactgc
     4261 gggctttacg ctgctgacgg catttttatt tcacagcaac tttgctgaag gcgtcaactc
     4321 gctgatgttc atgaaaaacc tgacaatttc tggcggattc ctgctgctgg caattaccgg
     4381 tccgggcgcg tatagcatcg accgcctgct gaataaaaag tggtaagcac gcgctatact
     4441 taacgataaa aaacgaggag gaagctcctc gtttttgcta ttggaggaga gaaaaatggg
     4501 tcaactgatt gacggcgtct ggcatgacac ctggtacgat accaaatcta ccggcggtaa
     4561 atttcaacgt tcagcttccg catttcgtaa ctggctcact gccgatggcg cacctggccc
     4621 cactggcaca ggcggtttta tcgcagagaa agatcgttat catctctatg tttcactcgc
     4681 ctgcccgtgg gcgcaccgca cgctgatcat gcgcaaactc aaaggactgg aaccgtttat
     4741 ttccgtttcc gtagtgaacc cgctgatgct ggaaaacggc tggacctttg atgacagttt
     4801 tccgggagca accggagata cgctctatca acatgaattt ctgtatcagc tttatctcca
     4861 cgccgatcca cactacagcg gacgagttac tgttcccgtg ctgtgggaca aaaagaacca
     4921 caccatcgtc agcaacgaat cagcagaaat catccgcatg ttcaataccg cgtttgatgc
     4981 gctgggcgca aaagcgggtg attactaccc accagccctg caaacgaaaa ttgacgaact
     5041 taacggctgg atttatgaca ccgttaacaa cggcgtgtat aaagctggtt ttgccaccag
     5101 ccagcaagcc tacgacgagg cggtggcgaa agtgtttgaa tcgctggcgc gactggagca
     5161 gattttaggc cagcatcgtt acctgaccgg caaccagcta accgaagccg atattcgcct
     5221 gtggactacg ctggtgcgtt ttgatccagt gtatgtgacc cacttcaagt gtgataagca
     5281 ccgcatcagc gattacctga atctgtatgg cttcctacgc gatatctacc agatgccggg
     5341 gatcgccgaa actgtcaatt tcgatcacat ccgtaatcat tacttccgca gccataagac
     5401 catcaaccct acggggatta tttcaattgg tccatggcag gatctcgatg aaccgcatgg
     5461 acgagatgtt cgcttcggtt aaaaaaaggc accttacgag gtgcctctga taattagcta
     5521 agtgcatgtt gttcatgtcg gatgcggcgt gaacgcctta tcaaacctac aaaacattta
     5581 tattcaatat gttgcaatga ccgcgcaggc cagataagcg tagcgcatct ggcgattttg
     5641 cgcttgtcat cagtcttagc gcgccttcac agcactttat taattccttg aacgaatatt
     5701 tactgccatt tcattcacgt ctattcttaa tttgctgctt taaaaacaag tgattgagca
     5761 aaattgaggc aaaaatggac tggtatctga aagtactaaa aaattatgtc ggtttccggg
     5821 gtcgtgcgcg gcgcaaagag tactggatgt ttattctggt caacatcatc tttacgttcg
     5881 tgctggggct gctggataaa atgttaggct ggcaacgcgc tggtggcgaa ggcatcctga
     5941 cgacaatcta cggtattctg gtgtttttac cgtggtgggc agttcagttc cgccgcctgc
     6001 acgacaccga ccgctcggcg tggtgggcac tgttgttctt aatcccgttt atcggctggc
     6061 ttattatcat cgtctttaac tgccaggcgg gtacgccagg cgaaaaccgc tttggaccag
     6121 atccgaaact ggaaccttaa aacaaatctg ctcgccggat gtctgactca tccggctttt
     6181 atcatcatgt
//
LOCUS       DAPHDW010000200         6066 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.203, whole genome shotgun sequence.
ACCESSION   DAPHDW010000200 DAPHDW010000000
VERSION     DAPHDW010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 6066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.203"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            306..437
                     /locus_tag="RH057_004180"
     CDS             306..437
                     /locus_tag="RH057_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
                     /protein_id="HDV3130463.1"
                     /translation="MIAYCFCEWHCSSRIMSVLADGVLVAGVVVVAFPLLKKKTPDC"
     gene            804..1232
                     /gene="tolA"
                     /locus_tag="RH057_004181"
     CDS             804..1232
                     /gene="tolA"
                     /locus_tag="RH057_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001479285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HDV3130464.1"
                     /translation="MGFRSASILTLIISGIIIGCTDAVSTNYHDRTSYYSDKAIETQY
                     VSSSERTSDVSEDIRLYAHQIKSAIEKQFGDASKYSGKECTLRMHMAPNGLLLEVKRE
                     SGDLDLCREAMNAIKNADIPAPPSPEVYKVFQNGVLDFKP"
     gene            1404..1778
                     /locus_tag="RH057_004182"
     CDS             1404..1778
                     /locus_tag="RH057_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001348108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tolA family protein"
                     /protein_id="HDV3130465.1"
                     /translation="MLTFWVGFVAGCTPLHPSDCHKTTATGSCSSGRWDDQDEWGAQA
                     RGIRAAINAKLDEPHNWKGKKCRLHMEFSQDGTALKISTSNGDKAYCEAIKSAAHKAK
                     FPAFNNPEVYRDFQKSGFDMRG"
     gene            2030..2245
                     /gene="essD"
                     /locus_tag="RH057_004183"
     CDS             2030..2245
                     /gene="essD"
                     /locus_tag="RH057_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV3130466.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
     gene            2250..2594
                     /locus_tag="RH057_004184"
     CDS             2250..2594
                     /locus_tag="RH057_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdfR family protein"
                     /protein_id="HDV3130467.1"
                     /translation="MTQKYELIVKGIRNFENKVTVTLALRDKERFDGEIFDLDISLDR
                     VEGAALEFYEAAARRSIRQVFLDVAAGLCEGDELSPEKRPIILEAQNVWITYKGKLPG
                     RITGSLKTPPKW"
     gene            complement(2560..2832)
                     /locus_tag="RH057_004185"
     CDS             complement(2560..2832)
                     /locus_tag="RH057_004185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130468.1"
                     /translation="MDNSTISLQELLDCISKLRDDVNALTVAFSYLAFSIPKEQMQPT
                     LASLQLESLNPKWSQQQQNSFKWLAILLEEKYAGEITISAESSENQ"
     gene            2938..3480
                     /locus_tag="RH057_004186"
     CDS             2938..3480
                     /locus_tag="RH057_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV3130469.1"
                     /translation="MNVKIRYGLSAAVLALIAVGAPAPDILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATVVDGKPVFPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNADDRKGACEAIRWWIKDGGRDCRLTKGQKNGC
                     YGQVERRDQESALACWGLEQ"
     gene            3477..3797
                     /locus_tag="RH057_004187"
                     /pseudo
     CDS             3477..3797
                     /locus_tag="RH057_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700651.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3799..5439
                     /locus_tag="RH057_004188"
                     /pseudo
     CDS             3799..5439
                     /locus_tag="RH057_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            5439..6020
                     /locus_tag="RH057_004189"
     CDS             5439..6020
                     /locus_tag="RH057_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3130470.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDEASWHLVEDHRGKTVYDVASGDALFISELGSLPENVTWLSP
                     EGEYQKWNGTAWVKDTEAEKLFRIREAEETKNNLMQVASEHIAPLQDAADLEIATEEE
                     ISLLEAWKKYRVLLNRVNTTTAPDIEWPVAPIG"
BASE COUNT         1643 a         1261 c         1563 g         1599 t
ORIGIN      
        1 gatgaggcgt cggggtgttt catccttagg cagcgtattg atatagtcaa tgcagcacga
       61 gcaaaggcct tcagccgttt gacagttttg ttctgtgctc ctgatcgtct ttcgggaaga
      121 gacgttatta ttctgaatag tgatgctata cagagggttt gcgatgagtt catggttgct
      181 aattcagaat tatttgctct tgttcaggag tacaacagaa tagccaggac ctgtggtatg
      241 gatgaacttc ggattactca tctggggtag atacatatct ggattatcac ctgttacggt
      301 aaaaagtgat tgcttactgt ttttgtgaat ggcattgcag cagccggata atgtcagtgc
      361 tggctgacgg tgtgctggtg gcgggtgtgg tagttgttgc tttcccgttg ctgaaaaaga
      421 aaacgccaga ctgttagccg ggtatcagtt agcgggagaa atttttaaat acttcacaat
      481 tcaggcggtt gactgttctc tggtttgcgg ggagtttgtt aaaagaaact ggcatggtga
      541 atccccctgt gcggaggggc aatcagcgag taggtatatg ggataatcgt ggattcaggt
      601 gctggtactg aattcaccgg gaggcccccg gcaccatgca cttcaataga ttctctccac
      661 attatggata ttctttcaga atatcccacg cagactttgt gtaaatgtta acaaatgtgc
      721 gttttatttg atctgattcg ctgtttgagc gtccaaaaca acggtatata taatccttta
      781 ctatatgaca tatgtgagga aaaatgggtt ttcgtagcgc atcaattctt acgttgatta
      841 ttagtgggat tattatcggg tgcactgatg ctgtatcgac aaattatcat gaccgtacat
      901 catattactc cgataaagca atagagacac agtatgtgag ttcatctgaa cgtacttctg
      961 atgttagtga ggatatccgt ctgtatgccc atcaaatcaa gagcgccatc gaaaaacagt
     1021 tcggggatgc gagtaagtat tcaggaaaag agtgtacact gagaatgcat atggccccga
     1081 atggccttct actggaggtt aaaagagaaa gtggagacct cgatttatgt cgtgaagcga
     1141 tgaatgcgat aaagaatgct gatatacctg cccccccttc gccggaagta tataaagtat
     1201 ttcaaaatgg ggtgctggat tttaaaccct gatatttatt gttttgtaat aaacggtttc
     1261 ggcttaggct tgttctgaca caactacggc actgagctaa atctagcgga tagtcagctc
     1321 tgagccagtg gcggacgtaa caactactat tgctgagatt ttaatggatt gaggagcaag
     1381 aagtgggatt aaagaaaatc gttatgttga ctttttgggt cggttttgtt gcgggatgca
     1441 cacctttaca cccttcagat tgccacaaaa ctactgctac aggtagttgc agttcaggac
     1501 gctgggatga tcaggatgaa tggggggcgc aagcgcgggg aatcagagct gcaattaatg
     1561 ccaaacttga tgagccgcat aactggaaag ggaaaaaatg caggttgcat atggaattct
     1621 ctcaggatgg cacggcgtta aaaatatcta ccagtaacgg tgataaagcc tattgcgaag
     1681 cgataaagtc cgcagctcat aaagccaaat ttccggcctt caacaatccg gaagtctaca
     1741 gagattttca gaaatctggc tttgacatgc gaggttagct cttcaattac tatatctcat
     1801 tcatagcaaa ctgacagatt tgatgatgtt ctatatacga aacctgtgat gtcaagtctg
     1861 agctaataca aataaacata atatcagaga aatacatttt attagctcgc tacggcgagc
     1921 tttttatatt gcatcgtctc cagcatatat atcaattaag gctccgattg atgtgtctgg
     1981 aagcctacac ataataacta tgccatccgt tccgtgcgga ggtgaggcta tgaaatccat
     2041 ggacaaaatt tcaacaggca ttgcctacgg cacctccgca ggcagtgctg gctactggtt
     2101 tttacagctg ctcgataaag tcacgccctc acagtgggca gcaataggtg tgctgggtag
     2161 cctggtattt ggcctgctga cgtacctgac aaacctttat ttcaagatta aagaagacaa
     2221 gcgtaaggct gcacggggag agtaattcaa tgactcaaaa atatgaactg attgtgaaag
     2281 ggatccgcaa ttttgagaat aaagttacgg taactttagc gttacgggac aaagaacgct
     2341 ttgacggtga aatttttgac ctggacatct cgctggaccg tgttgaaggt gccgcgctgg
     2401 agttttatga ggcagcagcc agaaggagca tcagacaggt cttcctggat gttgctgccg
     2461 ggttatgtga aggggatgag ctgtcgccgg aaaagcgccc cataatttta gaggcgcaga
     2521 atgtgtggat aacctacaaa ggaaagctac cgggaagaat tactggttct ctgaagactc
     2581 cgccgaaatg gtaatttcac cagcatattt ttcttccagt aatatcgcca gccacttgaa
     2641 agaattttgt tgttgctggg accatttggg gttgagtgat tcaagctgga gcgatgccag
     2701 tgttggttgc atttgttcct tgggaattga gaatgccaga tatgaaaatg cgacagtaag
     2761 ggcatttaca tcatcccgaa gcttggaaat gcagtcgagc aactcctgta gagaaatggt
     2821 gctattgtcc ataaacaatc ctctctattg tatttaacta ttccttgcct gattcaacag
     2881 gccgggacag ataaacatat ccagggttca gaaaccgata aatcctgata aaaatccatg
     2941 aacgtaaaaa tcagatacgg cctgtcggct gccgttctgg cactgattgc cgtcggtgcg
     3001 cctgcgcctg atattctcga ccagtttctg aatgaaaaag aaggtaacca cacaacggca
     3061 taccgcgatg gttccggcat ctggaccatc tgtcggggtg ccacggtggt ggatggaaaa
     3121 cccgtttttc ccggtatgaa actgtcgaag gaaaaatgcg accaggttaa cgccattgaa
     3181 cgggataagg cgctggcatg ggtggagcgc aatattaaag tgccactgac cgaaccacag
     3241 aaagcgggta tagcgtcatt ttgtccctat aacattggcc ccggtaagtg tttcccgtcg
     3301 acgttttata agcggctgaa tgccgatgat cgtaaggggg catgcgaggc gattcgctgg
     3361 tggataaaag atggtgggcg tgattgccgc ctgacaaaag ggcagaagaa tggctgttat
     3421 gggcaggtcg agcggcgcga tcaggaaagc gcactggcgt gctgggggct ggagcaatga
     3481 aaattaatcc ggatcttata ggcgttgtcg ttattgctgg cctttcggtc gctctcgtca
     3541 agagttgctc ttacgccagt aatcttcaga gcgataacga cgttctgcga agtgacaact
     3601 ctatgctggg gcaggtaatc gccacccagg cattcaactt caatcgattc aatcaggttg
     3661 cagaacatgc caacagtctt aactccctga tcgacaccag caccgaaaaa accgtaatcg
     3721 aataccggga gattctccgc cgtgaaaaaa cctgtgatct gcctgttcct gctgatatcg
     3781 ctggtgggtt gctcgaataa cggatttgtt atgcctggtg gctggactga cagggggcgt
     3841 tatgcttatg gaatgttctg gcaatatcaa aacaatgaac gagccatcca ctcaataatg
     3901 atgagtaata agggcgatga tttgcgctct gtgttctatg ttgatggcgc tgctttccct
     3961 gtttttgcgt ttatcgaaga tggcctgtca atatccgcac ctggtgctga tctcgttgtt
     4021 aatgatacga cctataagtt tggggcaaca aatccggcga ctgaatgtat cgcggcggac
     4081 gttatccttg attttaagag tgggcgtggt ttttatgagt ctcattcatt aatcgttaac
     4141 gataacttgt cgtgcaaaaa actttttgcc acagacgaaa ttgtagcgcg tggtggtaat
     4201 cagattcgaa tgataggtgg ggagtatggg gcattatggc gtaatgatgg cgctaaaact
     4261 tacctgctgc ttaccaatca aggtgatgtt tatggtggct ggaatacatt aagaccgttt
     4321 gctattgata acgcaaccgg cgaactggtt attggaacca aactgtccgc aagtctgaac
     4381 ggtaatgcat taacagcaac aaagctgcaa acgccaagac gggtttctgg tgttgagttt
     4441 gatggttcca aagatattac tttaaccgcc gcgcatgtgg ctgcttttgc cagaagggca
     4501 acggatacat atgccgatgc ggatggtggc gttccctgga atgccgaatc aggcgcttat
     4561 aatgtcaccc gctctggcga cagctatatt ctggttaact tctataccgg agtcggaagt
     4621 tgccggacct tgcagatgaa ggcgcattac agaaatggtg gtctgttcta ccgttcttca
     4681 agagacggtt atggttttga ggaagactgg gcagaagttt atacctcgaa aaatcttcca
     4741 ccagaaagct acccagtcgg cgcaccaatc ccgtggccat cagataccgt tccgtctggt
     4801 tatgccctga tgcaggggca gacttttgac aaatctgcct acccgaaact tgcagccgct
     4861 tatccgtcag gcgtgatccc tgatatgcgt ggctggacga ttaagggcaa gcccgccagt
     4921 ggtcgtgccg tattgtctca ggaacaggac ggcataaaat cgcataccca cagcgccagc
     4981 gcatccagta ctgatttggg gacgaaaacc acatcgtcgt ttgattacgg cactaaatcc
     5041 acgaataaca ctggtgcgca tacctatagt ttaagtggca gcacgaatgc agctggtaat
     5101 cacagccata gagatggccg tcgatttaac cccagtgttt ttaaagatac ttatcaatat
     5161 ggttatacaa gctcaggtca aaatacctgg ggtgtacaag gctcagtagg tatgtctacg
     5221 ggggcgttag ctaataccag tacagatggt aaccatagcc actcactgtc cggcacagca
     5281 gcatctgcag gtgcacacgc acatactgtc ggtattggtg cacacacgca ctccgttgcg
     5341 attggttcac atggacacac catcaccgtt aacgctgctg gtaacgcgga aaacaccgtc
     5401 aaaaacatcg catttaacta tattgtgagg cttgcataat ggcattcaga atgagtgaac
     5461 aaccacggac cataaaaatt tataatctgc tggccggaac taatgaattt attggtgaag
     5521 gtgacgcata tattccgcct catacaggtc tgccagcaaa cagtaccgat attgcaccgc
     5581 cagatattcc ggctggcttt gtggctgttt tcaacagtga tgaggcatcg tggcatctcg
     5641 ttgaagatca tcggggaaaa accgtctatg acgtggcttc cggcgacgcg ttatttattt
     5701 ctgaacttgg ctcgttaccg gaaaatgtca cctggttatc gccggaaggg gaatatcaga
     5761 agtggaacgg cacagcctgg gtgaaggata cggaagcaga aaaactgttc cggatccggg
     5821 aggcggaaga aacaaaaaac aacctgatgc aggtagccag tgagcatatt gcgccgcttc
     5881 aggatgctgc agatctggaa attgcaacgg aggaagaaat ctcgttgctg gaagcctgga
     5941 agaagtatcg ggtgttgctg aaccgtgtta atacaacaac tgcaccggat attgaatggc
     6001 cagtagcacc tatagggtaa attcgtaatg attacctaaa tacgttattc ttttgttcaa
     6061 aaagtg
//
LOCUS       DAPHDW010000201         5944 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.204, whole genome shotgun sequence.
ACCESSION   DAPHDW010000201 DAPHDW010000000
VERSION     DAPHDW010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5944
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.204"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(458..1264)
                     /locus_tag="RH057_004190"
     CDS             complement(458..1264)
                     /locus_tag="RH057_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase family protein"
                     /protein_id="HDV3130471.1"
                     /translation="MSGSVIHSQSAAMVPAVYSAGQSAQLPVAIDYPAALALRQMSMV
                     HDELPKYLLAPEVSALLHYVPDLRRKMLLATLWNTGARINEALALTRGDFSLTPPYPF
                     VQLATLKQRTEKAARTAGRMPAGQQTHRLVPLSDSWYVSQLQTMVATLKIPMERRNRR
                     TGRTEKARIWEVTDRTVRTWIGEAVAAAAADGVTFSVPVTPHTFRHSYAMHMLYAGIP
                     LKVLQSLMGHKSISSTEVYTKVFALDVAARHRVQFAMPESDAVAMLKQLS"
     gene            complement(1265..1570)
                     /gene="ccdB"
                     /locus_tag="RH057_004191"
     CDS             complement(1265..1570)
                     /gene="ccdB"
                     /locus_tag="RH057_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HDV3130472.1"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1572..1790)
                     /gene="ccdA"
                     /locus_tag="RH057_004192"
     CDS             complement(1572..1790)
                     /gene="ccdA"
                     /locus_tag="RH057_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HDV3130473.1"
                     /translation="MKQRITVTVDSDSYQLLKAYDVNISGLVSTTLQNEARRLRAERW
                     QAENQEGMAEVARFIEMNGSFADENRDW"
     gene            2418..2648
                     /locus_tag="RH057_004193"
     CDS             2418..2648
                     /locus_tag="RH057_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HDV3130474.1"
                     /translation="MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVR
                     PTWGSFLELEKADPDFMTEREDVVSDEGRVNL"
     gene            2645..3061
                     /locus_tag="RH057_004194"
     CDS             2645..3061
                     /locus_tag="RH057_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HDV3130475.1"
                     /translation="MNKIYMLDTNICSFIMREQPEALLKHLEQSVLRGHRIVVSAITY
                     SEMRFGATGPKASPRHVQLVDAFCERLDAVLPWDRAAVDATTEIKVALRLAGTPIGPN
                     DTAIAGHAIAACAILVTNNVREFERVPGLVLEDWVR"
     gene            3136..4701
                     /locus_tag="RH057_004195"
     CDS             3136..4701
                     /locus_tag="RH057_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HDV3130476.1"
                     /translation="MPLITRLMLQNFKKFPELDLRFSNDRNILVGDNESGKSTILLAL
                     DLVLSDSRHRVEALGVESLLSQSAVRHFQEGERRADQLPVLTADVFLSNGGEPDLNGR
                     QNLAGIDADGLRMRIAPMMEEYGQDIHHVLQQDPDNFPYEYYSVQFSTFSGGHFAGFR
                     RYLRHLFLDSARIDNEHAAQEYTRTVYSVNVPVADRYRLENSYRQQKMHFCARHLSAI
                     NDTLKTYQFGVRSGAKSGLEANLDITEDGISIRHRGKGRQCFIKTEFALQRHQQQGGE
                     IHALLLEEPENHLSHVSMKRLVNQLATERQTQVFIATHSSHISSRLDLRKAILLGATR
                     PVLMNELSAETAAFFMKAPDNNVLEFALARRVLLVEGDAEFILIEAFYHRLYGRAPED
                     DGVHIIAIGGTSFRRYLELARLLENRVAALRDNDGNYQQNCDERYADVLCSRSRVFAD
                     HDNSRSTFEICLYQDNADLCDALFRGTRRTLTVQDYMLANKAEAAFQLLQLHAEKLTV
                     PDYIQEALAWIRE"
     gene            4686..5708
                     /locus_tag="RH057_004196"
     CDS             4686..5708
                     /locus_tag="RH057_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrD"
                     /protein_id="HDV3130477.1"
                     /translation="MDKRVIFAVAGSGKTTLLIRRLREDRRTLILTFTVNNEAHLRAQ
                     IIRRFGYIPYGIRVMTWFEFLHGFCFRPFLQEQLSSRGLSFNQPPSRIPRTNIRHYQD
                     PAGRLYHRRLAHLLTARGLLPDIRTRLARYYDELFVDEVQDFAGHDFNFLLELCRAEI
                     SVLCCGDFYQHTFDTSRDGNVNATLHEDITRYEARFRAAGIMVDCETLSRTWRCSATV
                     CEFITGQLNIRISAHGTHTTQIEIVTDEARSAALHADNTMIKLFYREHHRYGCHSMNW
                     GGSKGLDHFQDVCIVMGANHWMRLIQQKLAALPPSSRNRLYVACSRARGNIYFIPESH
                     LRRFRN"
BASE COUNT         1440 a         1601 c         1529 g         1374 t
ORIGIN      
        1 tttcaggcat cgtgtgtaag cagaatatat aagtgctgtt ccctggtgct tcctcgctca
       61 ctcgagggct tcgccctgtc gctcgactgc ggcgagcact actggctgta aaaggacaga
      121 ccaaacatca tggttctgtg ttcattaggt tgttctgtcc attgctgaca taatccgctc
      181 cacttcaacg taacaccgca cgaagatttc tattgttcct gaaggcatat tcaaatcgtt
      241 ttcgttaccg cttgcaggca tcatgacaga acactacttc ctataaacgc tacacaggct
      301 cctgagatta ataatgcgga tctctacgat aatgggagat tttcccgact gtctcgttcg
      361 cttctcagtg gataacagcc agcttctctg tttaacagac aaaaacagca tatccactca
      421 gttccacatt tccatataaa ggccaaggca tttattctca ggataattgt ttcagcatcg
      481 caaccgcatc agactccggc atcgcaaact gcacccggtg ccgggcagcc acatccagcg
      541 caaaaacctt cgtgtagact tccgttgaac tgatggactt atgtcccatc aggctttgca
      601 gaactttcag cggtataccg gcatacagca tgtgcatcgc ataggaatgg cggaacgtat
      661 gtggtgtgac cggaacagag aacgtcacac cgtcagcagc agcggcggca accgcctccc
      721 caatccaggt cctgaccgtt ctgtccgtca cttcccagat ccgcgctttc tctgtccttc
      781 ctgtgcgacg gttacgccgc tccatgggta ttttcagtgt tgccaccatc gtctgcagct
      841 ggctgacgta ccaggagtca gagagcggaa ccagccggtg agtctgctga ccggcgggca
      901 ttctccccgc cgtcctggca gctttttcgg tccgttgttt cagggtcgca agctgcacaa
      961 acggatacgg aggcgtgagc gaaaaatccc cccgcgtcag cgctagtgct tcattaatac
     1021 gcgcgccggt gttccacaga gtggccagca gcatcttgcg gcgcagatcc gggacataat
     1081 gaagcagggc actcacctcg ggagccagca gatatttcgg cagttcatca tgaaccatcg
     1141 acatctggcg gagtgccaga gctgccggat aatcaatggc aacaggaagt tgtgcagact
     1201 gtccggcaga atacactgcc ggtaccatgg ctgcagactg gctgtgtata acggagcctg
     1261 acatttatat tccccagaac atcaggttaa tggcattttt gatgtcattt tctcggtggc
     1321 tgagatcagc cacttcctcc ccgataacgg agaccggcac actggccata tcggtggtca
     1381 tcatgcgcca gctttcatcc ccgacatgca ccaccgggta aagctcacgg gagaccttat
     1441 ctgacaacag acgtgcactg gccaggggga tcaccatccg tcgcccaggc gtgtcaataa
     1501 tgtcactctg gacatccaca aacagacgat aacggctctc tcttttatag gtgtaaacct
     1561 taaactgcat ttcaccagtc cctgttctcg tcggcaaaag agccgttcat ttcaataaac
     1621 cgggcgacct cagccatccc ttcctgattt tctgcctgcc agcgttcggc acgcagacga
     1681 cgggcttcat tctgcagggt ggtgcttacc agcccggaga tattgacatc atatgccttg
     1741 agcaactgat agctgtcact gtcgacggtg actgtaatgc gctgcttcat aatgcacctc
     1801 atcttacata ctctctgtac accattaagt atacaccagt attacgctat aacatgcctc
     1861 cggcaacgga atattgttat ccagctatta ttaacaccac catcaataac tgacctgaaa
     1921 agtaatcatg acaataataa cctgcaatgt agtaaccatg ataaattaca ggatgactgt
     1981 ttttctatga tccgattttt atcggattca ggattttccg gaagtgaatc aggatcagtg
     2041 aataagcgaa gcgatctttt ctgccgctga tgactggctc gggaacacgg gaagaaacac
     2101 aacatacagt gtcatgtcag cggagcggga caccacatcc tgtgaaagcg gaccagcggg
     2161 cggcagcgaa ggatccgcgg atccggaacc accagaaacc ggatctgcac ggggaaagag
     2221 agaaaataac ataggctgaa catatccata tgcctgacat ctgcatattc atggtatacg
     2281 catatcctca ccatctgcat atgctgaatc tatacatatg cctaacatct gcctatacta
     2341 aacatatgca cacacccggt gcaatgaaca tcattccgga tatgcatcac atatccacac
     2401 ttacccggga gagaatcatg agaaccgtat ccatttttaa aaatggcaac aaccgtgcta
     2461 tccgccttcc acgcgatctg gattttgagg gggtgagcga gctggagatc gtccgggaag
     2521 gggacagcat cattctgcgc cccgtccggc cgacctgggg ctcattcctg gagctagaaa
     2581 aggccgatcc ggactttatg acggagcgcg aggacgttgt cagcgacgaa ggacgagtta
     2641 acctgtgaac aagatctata tgctcgacac gaacatctgc tcgttcatca tgcgcgagca
     2701 acctgaagca ttgcttaagc acctggagca gtcggtgctg cgcggtcacc gtatcgtggt
     2761 ctcggccatc acctactccg agatgcgctt cggtgccacc ggtccgaagg cctcgccacg
     2821 ccacgtgcaa ctggttgacg cgttctgtga gaggctcgat gccgtcctgc cgtgggaccg
     2881 cgccgcggtg gatgcgacca cggagattaa ggtggcactg cgtctcgccg gcacgccgat
     2941 cggtccgaac gacacggcga tagccggaca cgccatcgct gcctgtgcca ttctggtgac
     3001 gaataatgtg agagagtttg agcgggtacc gggtcttgtg ctggaagact gggtaagata
     3061 acgctgtaaa acaaaacagg cggtcaggcg cggccggccg ctctttcagg cagcggtata
     3121 taaaggaata aaaaaatgcc actgatcacg cggcttatgc tgcaaaactt caaaaagttt
     3181 ccggaactgg acctgcgctt cagcaacgat cgtaacattc tggttgggga caatgagtcc
     3241 gggaaaagca ctattctgct ggctcttgac cttgtgctga gtgacagcag gcatcgggtt
     3301 gaggcgctgg gggtggaatc gctcctgtca cagtcagcag tcaggcattt tcaggaaggt
     3361 gagcgccgtg ccgatcagtt acccgtgctg accgctgacg ttttcctgag taatggggga
     3421 gagcctgacc tgaacggcag gcaaaatctg gcgggtatcg atgcggatgg tctcagaatg
     3481 cgcattgctc ccatgatgga ggaatacggt caggatatac accatgtttt gcagcaggat
     3541 cctgataatt ttccctatga atactattcc gtgcagttca gcacattctc cggtggtcat
     3601 ttcgcaggct tcagacgcta tctgcgccat ctgttcttag acagtgcacg cattgataac
     3661 gagcatgccg cacaggagta tacgcgcaca gtttacagcg tcaatgtgcc ggtggccgat
     3721 cggtaccggc ttgaaaacag ttaccggcaa cagaaaatgc acttctgcgc ccggcatctg
     3781 tccgccataa acgacacgct gaaaacgtat cagtttggcg tgcgttccgg cgctaaatca
     3841 ggtcttgagg cgaacctgga cattactgag gacggtattt ccattcgtca ccgcggaaaa
     3901 ggaagacagt gtttcatcaa aacggagttt gcgctgcagc ggcatcagca gcagggggga
     3961 gagatccacg cgctgttgct tgaggagccg gagaatcatc tcagccatgt cagtatgaag
     4021 aggctggtta atcagcttgc cacggaacgg cagacgcagg tgtttattgc cacacacagt
     4081 agccatatct cctcccgcct cgacctacgt aaggctattc tcctcggagc tacccggccg
     4141 gtactgatga atgaactttc cgctgagact gccgccttct tcatgaaagc accggataat
     4201 aacgtgctgg aatttgcgct ggccagacgc gtgctgctcg tggaggggga tgctgagttt
     4261 attctgatcg aggcctttta tcaccgcctg tatggcaggg caccggagga tgacggggtt
     4321 cacatcatcg ctatcggcgg aacgagtttc cgtcgttatc ttgaactggc ccgtcttctg
     4381 gaaaatcgtg tcgcggccct tcgcgacaat gacggcaact atcagcaaaa ttgcgatgaa
     4441 cgctatgctg acgtgctttg ttcccgctcc cgggtatttg ctgaccacga caacagccgc
     4501 tccacatttg aaatctgcct gtatcaggat aatgccgacc tgtgtgatgc gcttttccgc
     4561 ggaacccgcc ggacactcac ggttcaggat tacatgctgg ccaataaggc agaagcagca
     4621 ttccagctgt tacagttgca cgccgaaaag ctgaccgttc ctgattatat ccaggaggca
     4681 ctggcatgga taagagagtg atatttgcgg ttgccggctc gggtaaaacc acgctgctga
     4741 tacgtcggtt aagagaggac cggcggacgc tgatcctgac cttcaccgta aataatgaag
     4801 cacatctgcg ggcgcagatt atcagacgct ttggctacat accgtacggg atccgtgtga
     4861 tgacctggtt tgagtttctg cacggtttct gttttcgccc tttcctgcag gagcaactgt
     4921 catcgcgtgg tctgagcttc aaccagcccc cttccagaat accgcgcacg aatatccggc
     4981 attatcagga tcccgccgga cggctgtatc accggcggct cgcacacctg ctgacagccc
     5041 gggggttgct gccagatatc cgtacccgac ttgcccgtta ctacgatgag ttgttcgttg
     5101 atgaagtcca ggactttgcc ggccatgact ttaattttct gctggagcta tgccgcgcgg
     5161 aaatctcagt gctgtgctgc ggggattttt atcagcatac ctttgacaca agccgcgacg
     5221 gtaacgtcaa tgctacgctg catgaagaca taacccgata cgaggctcgc ttcagggctg
     5281 cgggtatcat ggtggattgt gagacgctca gccggacatg gcgctgttca gctacagtgt
     5341 gtgagttcat taccggacaa ctgaacattc gcatttcggc tcatggaaca cataccaccc
     5401 agattgaaat cgtcacggat gaagcgcgtt cagcggccct gcacgcggac aacacgatga
     5461 tcaagctctt ctatcgcgaa catcaccgtt acggatgcca ctcaatgaac tgggggggca
     5521 gtaaaggact cgaccacttt caggacgtct gcatcgtgat gggggccaac cactggatgc
     5581 gcctcatcca acagaagctg gcagcacttc caccatcatc ccgtaacagg ctatacgtgg
     5641 cctgctcacg ggcacggggc aacatctatt ttattccgga aagccatctt cggagattca
     5701 gaaactaagt cagcggcttc tcaactgggc acaagccggt aaaaagtagt caacgacaaa
     5761 acttcaggcc gaaaaatgag gctccggttt cgccggaaac cggacctgcg tggagggaaa
     5821 cccgcagatg atatgatatt tatcattaat cgcataataa atcctggcct gatatttaca
     5881 ggcagagaaa taaatcattc gatatatgat tttatcgtca tcagccatta accccccagg
     5941 aagc
//
LOCUS       DAPHDW010000202         5931 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.205, whole genome shotgun sequence.
ACCESSION   DAPHDW010000202 DAPHDW010000000
VERSION     DAPHDW010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.205"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            126..572
                     /locus_tag="RH057_004197"
     CDS             126..572
                     /locus_tag="RH057_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HDV3130478.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQDFIHQLQQGDADQVLALLNQPLSS"
     gene            583..2034
                     /locus_tag="RH057_004198"
     CDS             583..2034
                     /locus_tag="RH057_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HDV3130479.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISQALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            2024..3094
                     /locus_tag="RH057_004199"
     CDS             2024..3094
                     /locus_tag="RH057_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3130480.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GFKIPGVIGSVAPHALTEKQKQQPLSFDEMFIDIGANSREEAEKRGVEIGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAVLKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RDFLTTLTAEKVNAFSQFRQVD"
     gene            3094..4842
                     /locus_tag="RH057_004200"
     CDS             3094..4842
                     /locus_tag="RH057_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative frv operon regulatory protein"
                     /protein_id="HDV3130481.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPRLKQRYERTCCLASRPGLGHFIDETEEKRVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLMQGDYLSSLILAIYALRNQLTDIWPQYPGNEIKQIV
                     EQCGLFLGDNAVRTLTGLIEKQHQQAQVISADHVLGLLQRVPGIASLNIIDTQLVENI
                     TGHLLRCLAAPVWIAEHRQSSMNNLKAAWPAAFDMSLHFITLLREQLDIPLFDSDLIG
                     LYFACALERHQNERQPIILLSDQNAIATINQLAIERDVLNCRVIIARSLSELVAIREE
                     IEPLLIINNSHYLLEDAVNNYITVKNIITAAGIEQIKHFLATAFIRQQPERFFSAPGS
                     FHYSNVRGESWQHITRQICAQLVAQHHITADEAQRIIAREGEGENLIVNRLAIPHCWS
                     EQERRFRGFFITLAHPIEVNNEIINHVLIACAAADARHELKIFSYLASVLCQHPAEVI
                     AGLTGYEAFMELLHKG"
     gene            complement(4892..>5931)
                     /locus_tag="RH057_004201"
     CDS             complement(4892..>5931)
                     /locus_tag="RH057_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="YiiG family protein"
                     /protein_id="HDV3130482.1"
                     /translation="KRNLLSSAIIVAIMALGLTGCDDKKAETETPPPANSQPAAPAPE
                     APVAKAEAKPETPAQPVVDEQAVFDEKMDVYIKCYNKLQIPVQSSLARYADWLKDFKQ
                     GPTGEERTVYGIYGISESNLAECEKGVKSAVALTPALQPIDGVAVSYIDAAVALGNTI
                     NEMDKYYTQENYKDDAFAKGKTLHQTFLKNLEAFEAVAESYHAAIQEINDKRQLAELK
                     NIEEREGKTFHYYSLAVMISAKQINNLISQEKFDVDAAMKKVSELETLVAQAKEADKG
                     GMNFSFINSAGQYQLEAKKYVRRIRDKVPYSDWDKEQLQDANSSWMVDDSFPRALREY
                     NEMVDDYNSLR"
BASE COUNT         1383 a         1509 c         1498 g         1541 t
ORIGIN      
        1 attactccgc aaatctgacc tttcactctg tctcaaattg tcattgttta ccgacatatc
       61 gcctctcatc acttaatcgc tccaccatta caattgatta aaatatattt tatggagtcc
      121 gattaatggc agctcttact gcaagctgta ttgacctgaa tattcagggc aatggcgctt
      181 attccgttct gaagcagttg gcgacaatag cgttacaaaa cggttttatc accgactcac
      241 accagttcct gcaaaccctg ctgctgcgcg aaaaaatgca ctctactgga tttggttccg
      301 gtgtcgccgt gccgcacggt aaaagcgcct gcgttaaaca accgttcgta ttattcgccc
      361 gcaaagcgca ggctattgac tggaaagcca gcgatggcga agacgtcaat tgctggatct
      421 gcctcggcgt gccgcaaagc ggcgaagagg atcaggtcaa aatcatcggc acactgtgtc
      481 gcaaaattat ccaccaggat tttattcatc aactgcaaca gggcgacgcc gaccaggtgc
      541 ttgccttgtt aaatcaaccc ctcagctcat aaggaagtgg cgatggagtc atccttacgt
      601 attgtcgcga tcaccaactg ccccgccggg atcgctcaca cctacatggt ggcggaagcc
      661 ctggaacaga aagcccgttc tctcggccat accataaaag tggaaactca agggtccagt
      721 ggcgttgaaa accgcttatc cagcgaagag attgccgctg ccgattacgt cattctcgct
      781 accgggcgtg gcctgagcgg tgatgatcgc gcgcggtttg ccgggaagaa agtttatgag
      841 attgccatct cccaggcgtt gaaaaatatc gaccagattt tcagcgaatt accgacaaac
      901 tcgcagcttt ttgccgcaga tagcggcgtg aagcttggca aacaggaagt gcaaagcggc
      961 agcgtgatga gccacctgat ggcgggcgtc tctgcggcgc tgccgtttgt catcggcggt
     1021 ggtatcctgg tggcgcttgc caacatgctg gtgcagttcg gtttgccgta taccgatatg
     1081 tcgaaaggcg cgccgtcgtt tacctgggta gttgaatcca tcggttacct tggcttcacc
     1141 tttatgatcc ccatcatggg cgcttatatt gcctcgtcga ttgccgataa accggctttc
     1201 gctccggcgt ttctggtttg ctatctggcg aacgacaaag cgctgctcgg cacccagtcg
     1261 ggtgcgggat tcctcggcgc ggtggtgctg gggctggcga ttggctattt cgtcttctgg
     1321 ttccgcaaag tgcgccttgg caaagcatta caaccgctgc tcggttcaat gctgatcccg
     1381 ttcgttactc tgctggtttt cggcgtgctc acttactacg ttatcgggcc ggtgatgtcc
     1441 gacctcatgg gcgggctgct ccacttcctg aataccattc ctccatcaat gaagtttgcg
     1501 gcagcgtttc tggtcggtgc gatgctggcg ttcgatatgg gcggccccat caataaaacc
     1561 gcctggttct tctgcttctc actgctggaa aaacacattt atgactggta cgccatcgtc
     1621 ggcgttgtcg cgttgatgcc gcccgttgcg gcgggtcttg cgacgttcat tgcgcccaaa
     1681 ctgtttaccc gacaggaaaa agaggctgcc agtagtgcca ttgtggtcgg tgccacagtc
     1741 gcaaccgaac ctgctattcc ttacgctctg gcagctccgt taccgatgat taccgccaat
     1801 acgctggcgg gcggtattac cggcgtgctg gtgatcgctt tcggaatcaa acgtctggca
     1861 cctggtttgg gtatctttga cccgctgatc ggcctgatgt cgccggtagg ttcgttttat
     1921 ctggtgctgg cgattggcct cgcgctaaac atttcattca ttatcgtcct gaaaggattg
     1981 tggttacgcc gcaaagcgaa agccgcgcaa caggagcttg tccatgaaca ttgagttact
     2041 gcaacagttg tgcgaagcca gcgccgtcag cggcgatgaa caggaagttc gcgacatcct
     2101 gataaacacg ctggaacctt gcgttaatga aatcaccttt gatggtctgg gcagctttgt
     2161 tgcccgtaag gggaataaag gtccaaaagt tgccgttgtc gggcatatgg atgaagtcgg
     2221 ctttatggtc acccacatcg acgagagcgg ttttctgcgc ttcaccacca ttggcggctg
     2281 gtggaatcag tcgatgctca accaccgggt aaccatacgc acacacaagg gatttaaaat
     2341 ccctggtgtg attggttccg tcgcgcctca tgcgttaacg gaaaagcaaa agcaacaacc
     2401 gctgtcattt gatgagatgt tcattgatat tggcgcgaac agtcgcgaag aagcggaaaa
     2461 gcgcggcgtt gaaattggcg attttattag cccggaagcc aattttgcct gctggggcga
     2521 agataaagta gtcggaaagg cgctggataa ccgcatcggc tgcgcgatga tggcggagct
     2581 actacagaca gtaaataacc cagaaattac gctgtacggc gttggcagtg tggaagaaga
     2641 agttgggcta cgcggggcac aaacctcggc ggaacacatt aaaccggatg tggtgattgt
     2701 gctggatacc gctgtcgcag gtgatgttcc gggcattgat aacattaaat acccgctgaa
     2761 actgggccag gggccggggc tgatgctgtt tgacaagcgc tacttcccca accagaaact
     2821 ggtagcagtg ttaaaaaact gtgccgcaca taacgattta ccgctgcaat tttccaccat
     2881 gaaaaccgga gcgacggatg gcgggcgcta caacgtgatg ggcggcgggc gtccggttgt
     2941 cgcgctgtgt ctgccaactc gttatctgca cgctaacagc ggcatgattt caaaagccga
     3001 ttacgatgct ctgctcacgc tgatacggga ttttctgacg accttaactg cggagaaagt
     3061 caacgcgttt agccagttcc gtcaggtgga ttaatgctaa acgaacgcca gttaaagatt
     3121 gtcgatctgc tggagcaaca gccgcgcacg cctggagagc tggcgcaaca gactggcgtt
     3181 tcaggcagga ccatcctgcg tgatattgac tatctcaact tcacccttaa cggcaaagcc
     3241 cgcattttcg ccagtggtag tgcgggctat cagctggaaa tcttcgagcg ccgcagcttt
     3301 tttcagttgc tgcaaaagca cgataacgac gatcggctgc tggcgctatt attactaaat
     3361 acctttactc cccgtgcgca actcgcctcg gcgcttaatt tgccagaaac gtgggtagca
     3421 gaacgtctgc cccggttaaa acagcgttat gaacgcactt gttgcctggc cagtcgcccc
     3481 ggtttgggcc atttcattga tgaaacagaa gagaagcgcg ttatcttgct ggcgaacttg
     3541 ctacgcaaag atccgttttt aattccgctg gcgggcataa cacgagacaa ccttcagcat
     3601 ttatccacgg cctgcgacaa ccaacaccgc tggccgctga tgcagggtga ttatctctcc
     3661 agcctgattc tggcgattta cgccctgcgt aatcagctta ctgacatatg gccgcaatat
     3721 cccggtaacg agataaaaca aatcgttgaa cagtgcggcc tgtttctcgg tgataacgct
     3781 gtaagaaccc tgacgggttt gatagagaaa cagcatcagc aagcgcaggt aatttcagcc
     3841 gatcatgtgc tggggttgct gcaaagggtg ccaggcatcg cgtcattgaa tattattgat
     3901 acgcagttgg ttgagaatat taccgggcat ttattacgtt gccttgccgc accggtgtgg
     3961 attgctgagc accgccaaag cagcatgaat aacctgaaag ccgcctggcc tgcggccttt
     4021 gatatgagtc tgcactttat tacgctactg cgtgaacagc tcgatattcc ccttttcgac
     4081 agcgatctga tcggtttgta ttttgcctgt gcgctggaac ggcatcaaaa cgaacgccag
     4141 ccgatcattt tgctctcgga ccagaacgcg attgccacta ttaatcagct cgccattgag
     4201 cgcgatgttt taaattgtcg ggtaattatt gcccgtagct taagcgaact tgttgccatt
     4261 cgcgaagaga ttgagccgtt attgatcatt aacaatagcc attatttact ggaagacgcg
     4321 gtaaataatt acatcaccgt aaaaaatatc attacggctg ccggtatcga acaaataaaa
     4381 cattttctgg cgacggcatt tattcgccag caaccggagc gttttttctc tgcccccgga
     4441 agttttcatt attcgaatgt acgcggtgaa agctggcaac atattacccg gcaaatttgt
     4501 gcgcaattag tggcacaaca ccatattacc gccgatgaag cacaacgcat catcgcccgc
     4561 gaaggcgaag gtgaaaacct gattgttaat cgcctcgcca tcccacattg ctggagcgaa
     4621 caggagcgac gttttcgtgg attttttatt accctcgccc acccgatcga ggtgaataac
     4681 gaaatcatta accatgtgtt gattgcctgc gccgccgccg atgcgcgtca tgagctaaaa
     4741 atatttagct atctggcaag cgtattgtgt cagcatccgg cagaggttat tgccgggtta
     4801 acaggatatg aggcatttat ggagttactt cacaaggggt gaactgtgtc ggatgcgccg
     4861 catccgacac tgcatttatc atcaaaaacg tttaacgcag gctattatag tcatcaacca
     4921 tttcgttgta ctcgcgtaat gctctcggga aagagtcgtc gaccatccag cttgagtttg
     4981 cgtcctgcag ttgctcttta tcccagtcag agtacgggac tttatctctg atgcggcgaa
     5041 cgtatttttt agcctcgagt tgatactggc ctgccgaatt aataaacgag aaattcatgc
     5101 cgcctttatc cgcttctttg gcctgcgcca ctagcgtttc cagttcagac actttcttca
     5161 ttgccgcatc aacatcaaac ttctcttgcg atatcagatt attaatttgt ttcgctgaaa
     5221 tcatgactgc cagagagtaa tagtggaatg tttttccttc tctttcttca atatttttca
     5281 gttccgcaag ctggcgctta tcgttaattt cctgaatcgc cgcatgataa gattctgcta
     5341 cagcttcaaa ggcttccaga ttttttaaga atgtctggtg cagcgttttc cctttcgcaa
     5401 acgcatcgtc tttataattt tcctgggtgt aatatttatc catttcgtta atggtattac
     5461 ccaacgccac ggcagcatcg atataactca ccgccacgcc atcaataggt tgcagcgcag
     5521 gcgttaacgc cacagcactt tttacgcctt tttcacactc agcgaggttg gattcactaa
     5581 tgccgtagat gccataaaca gtacgctctt caccggtagg cccctgttta aaatctttca
     5641 gccagtcagc ataacgcgcc agactgctct gtacagggat ctgtaacttg ttatagcact
     5701 tgatatagac atccattttt tcgtcgaaaa ccgcttgttc atcgaccacc ggttgcgcag
     5761 gtgtttcagg tttagcttct gcttttgcaa cgggagcttc aggagctggt gcggcaggtt
     5821 gactattggc aggcgggggc gtttctgttt cggctttttt atcatcacaa cccgtcagac
     5881 cgagggccat gatggcgact ataatagcgg aagataataa attccttttc a
//
LOCUS       DAPHDW010000203         5895 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.206, whole genome shotgun sequence.
ACCESSION   DAPHDW010000203 DAPHDW010000000
VERSION     DAPHDW010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5895)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5895)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5895
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.206"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            57..1208
                     /gene="yiaY"
                     /locus_tag="RH057_004202"
     CDS             57..1208
                     /gene="yiaY"
                     /locus_tag="RH057_004202"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HDV3130483.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDSMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHIK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVAIYRAAM"
     gene            1373..2911
                     /gene="aldB"
                     /locus_tag="RH057_004203"
     CDS             1373..2911
                     /gene="aldB"
                     /locus_tag="RH057_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HDV3130484.1"
                     /translation="MTNNPPSAQIKPGEYGFPLKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(3130..3273)
                     /locus_tag="RH057_004204"
     CDS             complement(3130..3273)
                     /locus_tag="RH057_004204"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130485.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            3456..3779
                     /locus_tag="RH057_004205"
     CDS             3456..3779
                     /locus_tag="RH057_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HDV3130486.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     HVAGWVSLFTLHVIWPFLWIWATLYRPERGWGMQSHDSSVMQLQQRIAGLEKQLADIK
                     SSSAE"
     gene            3785..4921
                     /locus_tag="RH057_004206"
     CDS             3785..4921
                     /locus_tag="RH057_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HDV3130487.1"
                     /translation="MDLLIILTYVAFAWAMFKIFKIPVNKWTIPTAALGGIFIVSGLI
                     LLMNYNHPYTFKAQKAVISIPVVPQVTGVVIEVTDKKNTLIKKGEVLFRLDPTRYQAR
                     VDRLMADIVTAEHKQRALGAELDEMAANTQQAKATRDKFAKEYQRYARGSQAKVNPFS
                     ERDIDVARQNYLAQEASVKSSAAEQKQIQSQLDSLVLGEHSQIASLKAQLAEAKYNLE
                     QTIVRAPSDGYVTQVLIRPGTYAASLPLRPVMVFIPDQKRQIVAQFRQNSLLRLAPGD
                     DAEVVFNALPGKVFSGKLAAISPAVPGGAYQSTGTLQTLNTAPGSDGVIATIELDEHT
                     DLSALPDGIYAQVAVYSDHFSHVSVMRKVLLRMTSWVHYLYLDH"
     gene            complement(4918..5892)
                     /locus_tag="RH057_004207"
     CDS             complement(4918..5892)
                     /locus_tag="RH057_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3130488.1"
                     /translation="MTKLQLKYRELKIISVIAASENISHAATVLGIAQANVSKYLADF
                     ESKVGLKVFDRTTRQLMLTPFGTALLPYINDMLDRNEQLNNFIADYKHEKRGRVTIYA
                     PTGIITYLSKHVIDKIKDIGDITLSLKTCNLERNAFYEGVEFPDDCDVLISYAPPKDE
                     SLVASFITQYAVTAYASQRYLEKHPISRPDELEHHSCILIDSMMIDDANIWRFNVAGS
                     KEVRDYRVKGNYVCDNTQSALELARNHLGIVFAPDKSVQSDLQDGTLVPCFQHPYEWW
                     LDLVAIFRKREYQPWRVQYVLDEMLREIRHQLAQSQQLRPEQAAESED"
BASE COUNT         1454 a         1417 c         1542 g         1482 t
ORIGIN      
        1 cgaatgaaaa tcctcagtaa gctgcccacc cttttttaca ctttcaggag tgtgttatgg
       61 catcttcaac tttctttatt ccttctgtga atgtcatcgg cgctgattca ttgactgatg
      121 caatgaatat gatggcagat tatggattta cccgtacctt aattgtcact gacagtatgt
      181 taacgaaatt aggtatggcg ggcgatgtgc aaaaagcact ggaagaacgc aatattttta
      241 gcgttattta tgatggcacc caacctaacc ccaccacgga aaacgtcgcc gcaggtttga
      301 aattacttaa agagaataat tgcgatagcg tgatctcctt aggcggtggt tctccacacg
      361 actgcgcaaa aggtattgcg ctggtggcag ccaatggcgg cgatattcgc gattacgaag
      421 gcgttgaccg ctctgcaaaa ccgcagctgc cgatgatcgc catcaatacc acggcgggta
      481 cggcctctga aatgacccgt ttctgcatca tcactgacga agcgcgtcat atcaaaatgg
      541 cgattgttga taaacatgtc actccgctgc tttctgtcaa tgactcctct ctgatgattg
      601 gtatgccgaa gtcactgacc gccgcaacgg gtatggatgc cttaacgcac gctatcgaag
      661 cgtatgtttc tattgccgcc acgccgatca ctgacgcttg tgcactgaaa gccgtgacca
      721 tgattgccga aaatctgccg ttagccgttg aagatggcag taatgcgaaa gcgcgtgaag
      781 caatggctta tgcccagttc ctcgccggta tggcgttcaa taatgcttct ctgggttatg
      841 ttcatgcgat ggcgcaccag ctgggcggtt tctacaacct gccacacggt gtatgtaacg
      901 ccgttttgct gccgcatgtt caggtattca acagcaaagt cgccgccgca cgtctgcgtg
      961 actgtgccgc tgcaatgggc gtgaacgtga caggtaaaaa cgatgcggaa ggtgctgaag
     1021 cctgcattaa cgccatccgt gaactggcga agaaagtgga tatcccggca ggcctacgcg
     1081 acctgaacgt gaaagaagaa gatttcgcgg ttctggcgac taatgccctg aaagatgcct
     1141 gtggttttac taacccgatc caggcaactc acgaagaaat tgtggcgatt tatcgcgcag
     1201 cgatgtaatc atcatttcca caacggctgg caaattgtca gccgcttttt caactatctc
     1261 tgtaaccctt gcccgtaaat tcgtgatagc tgtcgtaaag ctgttaccga ctggcgaaga
     1321 tttcgccagt cacgtctacc cttgttatac ctcacaccgc aaggagacga tcatgaccaa
     1381 taatccccct tcagcacaga ttaagcccgg cgagtatggt ttccccctca agttaaaagc
     1441 ccgctatgac aactttattg gcggcgaatg ggtagcccct gccgacggcg agtattacca
     1501 gaatctgacg ccggtgaccg ggcagctgct gtgcgaagtg gcgtcttcgg gcaaacgaga
     1561 catcgatctg gcgctggatg ctgcgcacaa agtgaaagat aaatgggcgc acacctcggt
     1621 gcaggatcgc gcggcgattc tgtttaagat tgccgatcga atggaacaaa accttgagct
     1681 gttagcgacg gctgaaacct gggataacgg caaacccatt cgcgaaacca gtgctgccga
     1741 cgtgccgctg gcgattgacc attttcgcta tttcgcctcg tgtattcggg cgcaggaagg
     1801 tgggatcagt gaagttgata gcgaaaccgt ggcctatcat ttccatgaac cgttaggcgt
     1861 ggtggggcag attatcccgt ggaacttccc gctgctgatg gcgagctgga aaatggctcc
     1921 cgcgctggcg gcgggcaact gtgtggtgct gaaacccgca cgtcttaccc cgctttctgt
     1981 actgctgcta atggaaatcg tcggtgattt actgccgccg ggcgtggtga acgtggtcaa
     2041 cggcgcaggt ggggaaattg gcgaatatct ggcgacctcg aaacgcatcg ccaaagtggc
     2101 gtttaccggc tcaacggaag tgggccaaca aattatgcaa tacgccacgc aaaacattat
     2161 tccggtgacg ctggagctgg gcggcaaatc gccaaatatc ttctttgctg atgtgatgga
     2221 tgaagaagat gcctttttcg ataaagcgct ggaaggcttt gcactgtttg cctttaacca
     2281 gggtgaagtt tgcacctgtc cgagtcgtgc cttagtgcag gagtcaatct acgaacgctt
     2341 tatggaacgc gccatccgcc gtgtcgaaag cattcgcagc ggtaacccac tcgacagcgt
     2401 gacgcaaatg ggcgcgcagg tttctcacgg gcaactggaa accatcctca actacattga
     2461 tatcggtaaa aaagagggcg cagacgtgct caccggcggg cggcgcaagc tgctggaagg
     2521 tgaactgaaa gacggctact acctcgaacc gacgattctg tttggtcaga acaatatgcg
     2581 ggtgttccag gaggagattt ttggcccggt gctggcggtg accaccttca aaacgatgga
     2641 agaagcgctg gagctggcga acgatacgca atatggcctg ggcgcgggcg tctggagccg
     2701 caacggtaat ctggcctata agatggggcg cggcatacag gctgggcgcg tgtggaccaa
     2761 ctgttatcac gcttacccgg cacatgctgc gtttggtggc tacaaacaat caggtatcgg
     2821 tcgcgaaacc cacaagatga tgctggagca ttaccagcaa accaagtgcc tgctggtgag
     2881 ctactcggat aaaccgttgg ggctgttctg atataagaag ctggtcgcat tgggtattca
     2941 ttgcctgatg cgacgcttac gcgtcttatc atgcctacgg gaacctgacc gtaggccgga
     3001 taaggcgtac acgccgcatc cggcataaac aaagcgcacg ttgttacaat ttacgtttta
     3061 aacctacgtg cgcaataacc cagactacgc caacagaagt gcgtttctgg ggcgtgtttt
     3121 gtcaggagtt taatctcgcg tttcgatggt gagcgggcga accaggccgt aaatcaccat
     3181 tggtgatccg aggccaatca gcaataaacg gtagataatt gaaggtttaa tacgcttaat
     3241 ctgtactgtg gatgttttac tggagttatt cattagtttg gccaatagtt ccactatttc
     3301 aatatgataa acctcgtcct gttgaaaaac cagacagcac atttaagaaa gtatataaaa
     3361 aacaatatgt cgttatataa ataaggaaat tgcactgcat catggcattt cataatatgc
     3421 gttaattgtt cttttttata ttctttgagg gaaatatgtt cctggactat tttgcactgg
     3481 gagtgcttat ttttgtattt ctggtgatct tctatgggat cattatttta catgatattc
     3541 cctacctgat tgccaaaaaa cgtaatcatc ctcatgccga cgccattcat gttgctggtt
     3601 gggtgagtct ttttacgtta catgttatct ggccgttttt gtggatttgg gccacgcttt
     3661 accgcccgga gcggggatgg ggaatgcaaa gccatgattc atccgttatg caactgcaac
     3721 agcgcattgc cgggctggag aaacagctcg ccgacatcaa atcctcttct gccgagtaat
     3781 acttatggat ttactgatta ttttgaccta tgtggctttt gcatgggcaa tgtttaagat
     3841 cttcaaaatt cccgtaaata aatggaccat tcccacagcg gccctgggtg gaatatttat
     3901 tgtcagcggt ctaattctgt taatgaacta taaccatccg tataccttca aagcgcaaaa
     3961 agcggttatt tctattcctg ttgtcccaca ggtgacaggc gtggtgatcg aagtgacgga
     4021 taagaaaaat acgctgatta aaaaaggtga ggtgctattt cgactggacc cgacgcgtta
     4081 tcaggcgcgg gtggatcggc tgatggcgga tatcgttacc gcagaacata aacagcgggc
     4141 gttgggcgca gagttagatg agatggcggc gaatactcag caggcaaagg ccacgcggga
     4201 taaattcgct aaagagtatc agcgttacgc acgcggcagc caggcgaaag taaacccgtt
     4261 ttcagaacgc gatattgatg tggcgcggca aaattatctg gcgcaggaag cctccgtaaa
     4321 gtcatcggcg gcggaacaaa aacagatcca gagccagctg gatagcctgg tgttgggtga
     4381 acattctcaa atcgccagcc tgaaagcgca gctcgcggaa gcaaaatata accttgagca
     4441 gacgatagtg cgtgcgccga gcgatggtta tgtgacccag gtgctgattc gtccgggtac
     4501 ctatgccgcg tcgctgccgc tacgtccggt gatggtgttt atacccgatc agaaacgaca
     4561 aattgtggcg cagttccgtc agaactcctt gctgcgactg gcccctggcg acgatgcgga
     4621 agtggtgttt aacgctctgc caggcaaagt gttcagcggt aagctggcag ccattagtcc
     4681 agctgttccc ggcggagctt atcagtcgac cggcacctta cagacgttaa acacagcgcc
     4741 gggttcagat ggcgttatcg cgaccattga actggatgag cacactgatt tgagcgcttt
     4801 accagacggt atttacgccc aggtggcggt ctactctgat catttcagcc atgtctcggt
     4861 gatgcgcaaa gtgctgttac gcatgaccag ttgggtgcat tacctttatc tcgatcatta
     4921 atcttcgctt tccgccgctt gttcaggccg cagttgctgc gactgagcaa gctggtggcg
     4981 aatttcacgc agcatttcat ccagcacata ctggacccgc cagggctggt attcccgttt
     5041 acggaagata gccaccagat ccagccacca ttcatacggg tgctgaaagc agggcaccag
     5101 cgtgccgtct tgcagatcgc tctgcacact tttgtctggc gcaaacacaa tccccaggtg
     5161 atttcgtgcc agctccagcg ccgattgcgt gttatcgcaa acgtaatttc ccttcacgcg
     5221 ataatcccgc acctctttac tcccggcgac gttgaagcgc cagatattcg cgtcatcaat
     5281 catcatcgag tcgatcaaaa tacaggagtg atgttcgagt tcgtcaggac ggctaatagg
     5341 atgtttctca agatagcgct ggctggcata agcggttacg gcatattggg taataaaact
     5401 ggcaaccagc gattcatctt ttggtggagc gtagctgatt aaaacatcac aatcatcagg
     5461 aaattcgacg ccttcataaa aggcattacg ctcaagattg caggttttta gcgataaggt
     5521 gatatcaccg atatctttaa ttttatcgat tacatgtttg gataaatagg taattatgcc
     5581 ggttggggcg tagatggtga cccgaccacg tttctcatgc ttataatctg caataaaatt
     5641 attaagctgc tcgtttctgt ccagcatgtc gttgatgtac ggtaacagcg cggtaccaaa
     5701 aggtgtgagc atcagctgcc gggtggttcg gtcaaagact tttaaaccca cttttgattc
     5761 aaaatcagca aggtatttgc tgacgttggc ctgtgcgatg ccaagtacag tcgcggcatg
     5821 gctgatattt tcactggcag cgattaccga gataattttt aactcccggt acttaagttg
     5881 taattttgtc atcgc
//
LOCUS       DAPHDW010000204         5812 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.207, whole genome shotgun sequence.
ACCESSION   DAPHDW010000204 DAPHDW010000000
VERSION     DAPHDW010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5812)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5812)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5812
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.207"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(328..549)
                     /locus_tag="RH057_004208"
     CDS             complement(328..549)
                     /locus_tag="RH057_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000545700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130489.1"
                     /translation="MHFRKMKVVFLFIHILAVVMISVMTVHLCSMAVEKVPEECDIPV
                     RVAALVVLWLVFSGLIYWLFTAFMKTDVM"
     gene            complement(564..755)
                     /locus_tag="RH057_004209"
     CDS             complement(564..755)
                     /locus_tag="RH057_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130490.1"
                     /translation="MSDFVRHFSVKDKKEDVVMQDMHFLLEDFICAHISRKSGRVPGR
                     LRQRMDKLRLQRIRKDRTG"
     gene            complement(868..1902)
                     /gene="f17g"
                     /locus_tag="RH057_004210"
     CDS             complement(868..1902)
                     /gene="f17g"
                     /locus_tag="RH057_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F17G fimbrial adhesin"
                     /protein_id="HDV3130491.1"
                     /translation="MTNFYKVFLAVFILVCCNISQAAVSFIGSTENDVGPSPGSYSRT
                     HAMDNLPFVYNTGNNIGYQNANVWRISKGFCVGLDGKVDLPVVGSLDGQSIYGLTEEV
                     GLLIWMGDTNYSRGTAMSGNSWENVFSGWCVGANTASTQGLSVRVTPVILKRNSSARY
                     SVQKTSIGSIRMRPYNGSSAGSVQTTVNFSLNPFTLNDTVTSCRLLTPSAVNVSLAAI
                     SAGQLPSSGDEVVAGTTSLKLQCDAGVTVWATLTDATTPSNRSDILTLTGASTATGVG
                     LRIYKNTDSTPLKFGPDSPVKGNENQWQLSTGTETSPSVRLYVKYVNTGEGINPGTVN
                     GISTFTFSYQ"
     gene            complement(1904..4372)
                     /locus_tag="RH057_004211"
     CDS             complement(1904..4372)
                     /locus_tag="RH057_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3130492.1"
                     /translation="MLSVPLWAAEDAEFDPSFLSRTPGSSVIDVRRFTHGNPVPSGNY
                     YSDIYLNGEWKGNAELSFRDMSSSQEESVLCVTPELLSILDVSPEAVAREPDTGNGRC
                     MPFSAVVPDAKIRFDLSLLRLNVEIPQAMLVSRPRGYISPSQWQTGVPAAFVSYDVNH
                     WRYRTPDMENNQSYLGLRAGFNAGGWAFRHRGTESWSDGHAEGYRSIETNLQHDIAML
                     RAQLTLGDFTTTGELMDSNSLRGIRLASDDRMLPGSLRGYAPVVRGMAGSNARVTVRQ
                     NGNIIYETTVPAGPFSISDLYPSGYGGDLTVTVTESDGQTRSFIVPFASVAQLVRPGF
                     SRWQVAAGRYRYGNRTFSDTVFQGTLQYGLTNDITLNTGMSTAPHYLSGLGGVAFNTP
                     LGAVATDITLARTTFPGSDTTHRGYSLHAGYSARIPETSTNVTLAAYRYSSRDFWSLR
                     DAILTRNRDVINDSSVRSATGYRPRNQLQLTVNQSLGEGWGNLYLTGATYHYWGHTGT
                     FNEYQAGYNNVWKKMSYQIGFSQTRRSGDYHHDNRFFFSFSLPLSDTGRTLLSTTVNV
                     SDKNSNSIQSSISGVSGDDNQLSYGVSLNTQENGPTGYAVNGSYRSPVATLQATAGND
                     SRHNRQISAGISGAIVAHPFGVTASNDLGDTFTVIHADGAGGAVINNAPGNRLDPWGN
                     GIVPYVTPYEKNYISIDPTALAADVELSATEREIIPRANSTTMVTFTTKTGKAMLFDV
                     KMPDGTPPPMAAEALTADGKSAGYIAQGGRLFVRDLDAEKGMLRIVWGSGQNDSCVIH
                     YSASGENARGNAVPENHHVVCMRQ"
     gene            complement(4455..5177)
                     /locus_tag="RH057_004212"
     CDS             complement(4455..5177)
                     /locus_tag="RH057_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3130493.1"
                     /translation="MKHILLFIFTVLLSLPSYGSVVIMGTRVIYPAEQKSINVRLNNG
                     DSTPSLIQAWLDTGDPSSPPDSVRVPFIITPPVFRMEPLSGQTMRIMYTGEKLPADRE
                     SLFWLNVLDIPAKPSFAGKSEKAQGYNYLQFAVRSRIKFFFRPDGLPFSPDDAYKKVT
                     WQLTGGDTLQASNPTPYFITFSTVSVGQGAQKVSSTQGGMAAPFSSVTFRLKQKVSPS
                     GKVEWTVVNDNGGYQKGESVLK"
     gene            complement(5245..5790)
                     /gene="f17a"
                     /locus_tag="RH057_004213"
     CDS             complement(5245..5790)
                     /gene="f17a"
                     /locus_tag="RH057_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021578761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F17A fimbrial adhesin"
                     /protein_id="HDV3130494.1"
                     /translation="MRKIQFILGILAAASSSATLAYDGTITFNGKVVDQTCSVTTESK
                     NLTVTLPTVSSNSLTSNGEVAGLTPFTISLEGCTSPASAGAENVKAYFEPNATTDYDS
                     HNLNIASGSGNATNVQIQLLNADGTTPILLGTDSAGQAVTPVAINGTNVTLRYNARYY
                     ATGQATAGNVSATVNYTIAYQ"
BASE COUNT         1561 a         1535 c         1271 g         1445 t
ORIGIN      
        1 cattcagacg aagagatttg atttacacgg taagagcata tggcacatta caacaggggt
       61 ctctgtgaat atatacagaa aacatatctc ataaaacccg ggaaatgccc catgaataca
      121 ggacagtgaa aacaggcatg ataatcagga aagccatgcc tgatacagaa aaagttacct
      181 gcggggataa tgcatgcgta ttttcatgga aggagatcac tcaataactt ccatcgagag
      241 cgggtaataa ctcaccgatc tccttcacgg taataagcaa tgcacaacca ggcacttact
      301 ggccggttat ccatgtctat attatattta catcacgtca gttttcataa aggctgtgaa
      361 cagccagtaa atcaggccac tgaataccag ccacagcaca accagggctg caaccctcac
      421 agggatatca cactcttccg gcactttttc cacagccata ctgcacagat ggactgtcat
      481 aacagaaatc atcacaacag caaggatatg aatgaaaaga aaaacgactt tcattttccg
      541 gaaatgcata tacaaaccct gactcagcct gtcctgtctt tcctgatcct ctgtaatctt
      601 aacttatcca tgcgctgacg aagtctgccc ggaacacgcc cgctctttct tgaaatatga
      661 gcacagataa aatcttcaag cagaaaatgc atgtcctgca ttaccacatc ttcttttttg
      721 tctttcactg aaaaatgcct gacaaaatcg ctcatgaaaa tgtttctgaa acgctgttat
      781 ccagcttcag aaaccggtac tgccttcacc aacacaaatc attttgttgt cactgtctga
      841 cctgttctcc cctcccggaa ctcgctgtta ctgataggaa aatgtaaatg ttgatattcc
      901 gttaaccgta cccggattaa ttccctcacc agtattcaca tactttacat acaaccggac
      961 tgagggtgac gtttccgttc ctgttgataa ctgccactgg ttttcatttc cctttaccgg
     1021 cgaatcaggt ccaaacttca ggggcgtact gtcagtgttt ttgtatattc tcagcccgac
     1081 tccggttgca gtcgatgccc ccgtcagtgt gagtatatcg cttctgttgg acggtgtggt
     1141 cgcatcagtc agtgttgccc atactgttac tccggcatca cactgtaatt tcagtgatgt
     1201 tgtcccggcg acaacttcat caccggatga tggcagttgt ccggcagaaa ttgcagccag
     1261 gctgacattg actgcggaag gtgtcagtaa tctgcacgat gttactgtgt cattcagcgt
     1321 aaatggattc aggctgaaat tcactgtggt ctgaacactg cctgcagatg aaccgttata
     1381 gggcctcatt ctgatactcc cgatgctggt cttctgtaca ctgtatcgcg cagaggaatt
     1441 tctttttaaa attacaggtg ttacacgaac agacagtccc tgggttgatg cagtattagc
     1501 tccgacacac catccggaaa agacattttc ccatgagttt ccgctcatcg cggtacccct
     1561 ggaataattc gtgtccccca tccatataag gagccccacc tcctctgtca gcccataaat
     1621 actctgcccg tcaagactgc caaccacggg taaatccact ttcccgtcca gcccgacaca
     1681 aaatccctta gaaatacgcc agacatttgc attctgatat ccaatgttgt taccggtatt
     1741 atagacaaat ggcaggttat ccattgcatg agttctggaa taagagcctg gagatggtcc
     1801 cacatcgttt tccgtactgc caataaatga aactgccgcc tgactgatat tgcagcaaac
     1861 cagaatgaat acagccagaa agaccttata aaaatttgtc atattattgc ctcatgcata
     1921 ctacatgatg gttttccgga acagcatttc ccctggcatt ttctccggac gcactatagt
     1981 gaatgacaca actgtcattc tgcccgcttc cccagactat cctgagcatc cctttttcag
     2041 catccaggtc acgtacaaat aaccggcctc cctgagcaat gtagcctgca gatttcccgt
     2101 ctgctgttaa tgcttccgcg gccatcggcg gcggcgttcc gtccggcatc ttcacatcaa
     2161 acaacatggc tttccccgtt tttgttgtga aggtcaccat ggtggtactg ttggcccggg
     2221 gaataatttc ccgctctgtt gcagacagtt ccacatccgc agccagcgct gtcgggtcga
     2281 tactgatata attcttttcg taaggtgtga catacggaac aatgccgtta ccccaggggt
     2341 ccagccggtt acccggcgca ttattaatca ccgccccacc ggcaccatca gcatgaataa
     2401 cggtaaatgt gtcaccaagg tcattactgg ccgtcacacc aaacggatgc gccactattg
     2461 ccccggatat accggcagat atctgacggt tatggcgact gtcattacct gccgtggcct
     2521 gcagggtggc caccggtgaa cgataactgc cattcaccgc ataccctgtc ggtccgtttt
     2581 cctgggtgtt cagggacaca ccatatgaca actgattgtc atccccgctg acaccactga
     2641 tactgctctg aatgctgtta ctgtttttat cgctgacatt cactgtcgtg gacagcaacg
     2701 tccttcccgt atctgaaagt ggcagggaaa aactgaaaaa gaaccggtta tcatgatgat
     2761 aatcaccact ccgtcgggtc tgggaaaagc caatctggta actcattttt ttccagacgt
     2821 tgttataacc ggcctggtac tcattgaatg tccctgtatg cccccagtaa tgataagtgg
     2881 ctccggtcag atacaggttt ccccatccct caccaagact ctggttcaca gtcagttgca
     2941 actgattcct tggccggtaa ccagttgcac tcctgaccga tgaatcatta atcacatcac
     3001 gattcctggt caggatggca tcccgaagac tccagaagtc acgggatgaa taacggtaag
     3061 ccgccagcgt cacatttgta ctggtttccg gtattcgtgc actgtaaccg gcatgcaggc
     3121 tgtagccgcg atgtgtcgtg tcagaacccg ggaaagtcgt tctggccagc gtaatatccg
     3181 tggcaacagc ccccagtggt gtattgaatg ccacaccacc cagccctgac agataatgag
     3241 gtgcggtact catccccgta ttcagggtga tatcatttgt cagcccgtac tgcagggtcc
     3301 cctggaatac ggtatcgctg aatgtcctgt taccgtaacg ataacgcccg gctgccacct
     3361 gccagcgact gaaccccggg cgaaccagtt gtgcaaccga agcaaacggc acgataaaac
     3421 tcctggtctg tccgtctgac tctgtcacag tgactgtcag gtcaccacca tatccactgg
     3481 gataaaggtc actgatggag aaaggccctg ccggcacggt agtttcataa ataatgttac
     3541 cgttctgccg gacagtaacc ctggcgttac ttccggccat cccccgaacc acaggggcat
     3601 aaccacgcaa cgaacccggc agcatccggt catcagaagc cagtcttatt cccctgaggc
     3661 tgttactgtc cataagctca cctgtggtgg tgaaatcccc cagtgtcagt tgcgcacgca
     3721 acatggctat gtcgtgctga aggttcgttt caatactccg gtaaccttct gcatgaccgt
     3781 cactccagct ttctgtcccc cggtgacgaa atgcccagcc tcctgcgtta aagccggcac
     3841 gaagaccaag ataactctga ttattttcca tatcgggcgt ccggtaacgc cagtgattca
     3901 cgtcataact gacaaaggca gccggcaccc ccgtctgcca ctgagaaggt gatatgtatc
     3961 cccgtggacg ggaaaccagc atggcctgcg gaatttcaac attcagccgc agcagtgaca
     4021 ggtcaaaacg gatttttgca tcaggtacaa ctgcagaaaa tggcatgcaa cggccatttc
     4081 cggtatccgg ttcacgggca actgcctcgg gtgacacatc cagaatggat aacagttccg
     4141 gcgtcacaca cagaacactc tcctcctgtg aagaggacat atcacggaat gacaattccg
     4201 catttccttt ccattcaccg ttcagataaa tgtctgaata ataattccct gagggaacag
     4261 gattcccgtg cgtgaaacga cggacatcaa tcacactgct ccccggcgtg cggctcagaa
     4321 aactcgggtc aaattctgca tcctctgccg cccacaaagg cacagacagc atatacagtc
     4381 ccgacagaca acataaataa atgacacagt acttcagacc ggataaagaa taatgactca
     4441 cttacgggcc cttattattt cagaacggat tcacctttct ggtaaccacc attatcattc
     4501 accaccgtcc attccacttt tcctgacgga gaaaccttct gcttaagtct gaaagtgaca
     4561 gaagaaaacg gtgcggccat tcccccctga gtactgctga ctttctgagc tccctgtccg
     4621 acactgacag tactgaaggt aataaaataa ggcgtgggat tactggcctg aagggtatca
     4681 ccgcctgtaa gctgccatgt cactttctta tatgcatcat caggagaaaa aggcagacca
     4741 tcaggacgga aaaaaaactt aatccggctg cgtacggcaa actgaaggta attatatccc
     4801 tgtgcttttt cagattttcc ggcaaaagaa ggtttggccg gaatatcaag aacattcagc
     4861 cagaacagtg attccctgtc tgccggaagt ttttctcccg tatacataat tcgcattgtc
     4921 tgtccggaaa gcggctccat ccggaaaaca ggaggcgtaa taataaacgg caccctgaca
     4981 gagtcaggag gggaagacgg gtcccctgta tcaagccatg cctgtatcag ggatggcgta
     5041 ctgtctccgt tattcagccg gacattaata cttttttgtt ctgcaggata aataaccctg
     5101 gtccccataa taaccacact gccatatgag ggcagagaca aaagaacggt aaaaatgaac
     5161 agcagaatat gtttcataca ggctccgtaa acacggggca aaatgccccg tgaacactta
     5221 agaaggcagg tattaagata aaaattactg ataagcgatg gtgtaattca cagtggcaga
     5281 aacgttgccg gcggtggcct gacccgttgc atagtaccgt gcgttataac gtaacgtcac
     5341 atttgtacca ttaatagcaa caggtgttac cgcctgacca gcagaatcag taccgagtaa
     5401 tatcggtgtg gtaccatcgg cattcagaag ctggatctga acatttgttg cattaccaga
     5461 gccgcttgca atattaaggt tatgactatc ataatcagtg gtcgcattag gctcaaaata
     5521 ggccttcaca ttctcagcac ctgctgaagc aggactggta cagccttcca gtgaaattgt
     5581 gaaaggggta aggcctgcca cctccccatt tgaagttaat gaattagagg agacagttgg
     5641 tagcgtaact gtcaaattct tgctttctgt cgtaacagaa caagtttgat caacaacttt
     5701 tccattaaaa gtaattgtac cgtcataagc aagcgtagca gaagatgacg cagccgccag
     5761 tattccaagg ataaattgaa ttttccgcat agaatcctca gtgaaactga ta
//
LOCUS       DAPHDW010000205         5782 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.208, whole genome shotgun sequence.
ACCESSION   DAPHDW010000205 DAPHDW010000000
VERSION     DAPHDW010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5782
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.208"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            171..821
                     /gene="csgD"
                     /locus_tag="RH057_004214"
     CDS             171..821
                     /gene="csgD"
                     /locus_tag="RH057_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HDV3130495.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            826..1215
                     /gene="csgE"
                     /locus_tag="RH057_004215"
     CDS             826..1215
                     /gene="csgE"
                     /locus_tag="RH057_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HDV3130496.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDFEKTVVFAL
                     IQTEEALNRRQINQALLSTGDLAHDEF"
     gene            1240..1656
                     /gene="csgF"
                     /locus_tag="RH057_004216"
     CDS             1240..1656
                     /gene="csgF"
                     /locus_tag="RH057_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HDV3130497.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            1683..2516
                     /gene="csgG"
                     /locus_tag="RH057_004217"
     CDS             1683..2516
                     /gene="csgG"
                     /locus_tag="RH057_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
                     /protein_id="HDV3130498.1"
                     /translation="MQRLFLLVAVMLLSGCLTAPPKEAARPTLMPRAQSYKDLTHLPA
                     PSGKIFVSVYNIQDETGQFKPYPASNFSTAVPQSATAMLVTALKDSRWFIPLERQGLQ
                     NLLNERKIIRAAQENGTVAINNRIPLQSLTAANIMVEGSIIGYESNVKSGGVGARYFG
                     IGADTQYQLDQIAVNLRVVNVSTGEILSSVNTSKTILSYEVQAGVFRFIDYQRLLEGE
                     VGYTSNEPVMLCLMSAIETGVIFLINDGIDRGLWDLQNKAERQNDILVKYRHMSVPPE
                     S"
     gene            complement(2580..3071)
                     /locus_tag="RH057_004218"
     CDS             complement(2580..3071)
                     /locus_tag="RH057_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HDV3130499.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(3173..3727)
                     /gene="ycdY"
                     /locus_tag="RH057_004219"
     CDS             complement(3173..3727)
                     /gene="ycdY"
                     /locus_tag="RH057_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HDV3130500.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMAQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMPL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(3751..4488)
                     /gene="ycdX"
                     /locus_tag="RH057_004220"
     CDS             complement(3751..4488)
                     /gene="ycdX"
                     /locus_tag="RH057_004220"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HDV3130501.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMIATIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPPER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(4543..5481)
                     /gene="ghrA"
                     /locus_tag="RH057_004221"
     CDS             complement(4543..5481)
                     /gene="ghrA"
                     /locus_tag="RH057_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
                     /protein_id="HDV3130502.1"
                     /translation="MDIIFYHPTFDTQWWIEALRKAIPQARVRAWKSGDNDSADYALV
                     WHPPVEMLAGRDLKAVFALGAGVDSILSKLQAHPEMLNPSVPLFRLEDTGMGEQMQEY
                     AVSQVLHWFRRFDDYRIQQNSSHWQPLPEYHREDFTIGILGAGVLGSKVAQSLQTWRF
                     PLRCWSRTRKSWPGVQSFAGREELSAFLSQCRVLINLLPNTPETVGIINQQLLEKLPD
                     GAYLLNLARGVHVVEDDLLAALDSGKVKGAMLDVFNREPLPPESPLWQHPRVTITPHV
                     AAITRPAEAVEYISRTIAQLEKGERVCGQVDRARGY"
BASE COUNT         1568 a         1419 c         1337 g         1458 t
ORIGIN      
        1 tttgtaatgg ctagattgaa atcagatgta atccattagt tttatatttt acccatttag
       61 ggctgattta ttactacaca cagcagtgca acatctgtca gtacttctgg tgcttctatt
      121 ttagaggcag ctgtcaggtg tgcgatcaat aaaaaaagcg gggtttcatc atgtttaatg
      181 aagtccatag tattcatggt catacattat tgttgatcac taaaccgtct ttgcaagcga
      241 cagctctctt gcagcacctt aaacaatcgc tggcaattac aggaaaatta cataatattc
      301 aacgttctct ggacgatata tcttcaggct ctattattct tctggatatg atggaagcag
      361 ataaaaagct tatccattat tggcaggata ctttgagcag gaaaaacaac aatatcaaaa
      421 tattgttgct aaatacgcct gaagattacc cgtaccgcga cattgaaaac tggcctcata
      481 tcaacggcgt tttttatgcc atggaggatc aagaacgtgt tgtcaatggg ttgcaaggcg
      541 tcctgcgcgg cgaatgctac tttacgcaaa agcttgccag ttacctgatt acacattctg
      601 gtaactatcg ttataacagc acggaatcag ccctccttac tcatcgggaa aaagagatcc
      661 tgaataagct gcgtatcggc gcgtctaata acgagatcgc tcgttcgttg ttcatcagcg
      721 aaaatacggt taaaacgcat ctttataatc ttttcaagaa gatagccgta aaaaaccgga
      781 cacaagcggt ttcctgggca aacgataacc tcaggcgata aagccatgaa acgttattta
      841 cgctggattg tggcggcaga atttctgttc gccgcaggga atcttcacgc cgttgaggta
      901 gaagtcccgg gattgttaac tgaccatact gtttcatcta ttggccatga tttttaccga
      961 gcctttagtg ataaatggga aagtgactat acgggtaact taacgattaa tgaaaggccc
     1021 agtgcacgat ggggaagctg gatcactata acggtcaatc aggacgttat tttccagact
     1081 tttttatttc cgttgaaaag agacttcgag aaaactgtcg tctttgcact gattcaaact
     1141 gaagaagcac taaatcgtcg ccagataaat caggcgttat taagtacggg cgatttggcg
     1201 catgatgaat tctaaataaa aaattgttcg gaggctgcaa tgcgtgtcaa acatgcagta
     1261 gttctactca tgcttatttc gccattaagt tgggctggaa ccatgacttt ccagttccgt
     1321 aatccaaact ttggtggtaa cccaaataat ggcgcttttt tattaaatag cgctcaggcc
     1381 caaaactctt ataaagatcc gagctataac gatgactttg gtattgaaac accctcagcg
     1441 ttagataact ttactcaggc catccagtca caaattttag gtgggctact gtcgaatatt
     1501 aataccggta aaccgggccg catggtgacc aacgattata ttgttgatat tgctaaccgc
     1561 gatggtcaat tgcagttgaa cgtgacagat cgtaaaaccg gacaaacctc gaccatccag
     1621 gtttcgggtt tacaaaataa ctcaaccgat ttttaagccc cagcttcata aggaaaataa
     1681 tcatgcagcg cttatttctt ttggttgccg tcatgttact gagcggatgc ttaaccgccc
     1741 cgcctaaaga agccgccaga ccgacattaa tgcctcgagc tcagagctac aaagatttga
     1801 cccatctgcc agcgccgtcg ggtaagatct ttgtttcggt atacaacatt caggacgaaa
     1861 ccgggcaatt taaaccctac ccggcaagta acttctccac tgctgttccg caaagcgcca
     1921 cggcaatgct ggtcacggca ttgaaagatt ctcgctggtt tataccgctg gagcgccagg
     1981 gcttacaaaa cctgcttaac gagcgcaaga ttattcgtgc ggcacaagaa aacggcacgg
     2041 ttgccattaa taaccgaatc ccgctgcaat ctttaacggc ggcaaatatt atggttgaag
     2101 gttcgattat cggttatgaa agcaacgtta aatcgggcgg tgttggggca agatattttg
     2161 ggatcggtgc agacacgcaa taccagctcg atcagattgc cgtgaacctg cgcgtcgtca
     2221 atgtgagtac cggtgagatc ctctcctcgg tgaacaccag taagacgata ctttcctatg
     2281 aagttcaggc cggggttttc cgctttattg actaccagcg cttgctggaa ggggaagtgg
     2341 gttacacctc aaacgaaccc gttatgctgt gtctgatgtc ggctatcgaa acaggggtca
     2401 ttttcctgat taatgatggt atcgaccgtg gtctgtggga tttgcagaac aaagcagaac
     2461 ggcagaacga cattctggtg aaataccgcc atatgtcggt tccaccggaa tcctgacaga
     2521 taaaaaagcc ggaattatca tccggcattt tactgtttaa gcgaaagcgt gagtgcccct
     2581 cacgctttat ttttgatttc ctgctcacgg tgagccgtct ttgcactacg cgccgccagc
     2641 cacaggccgc tgtttttcat tgcgtaacca aataccagcc caagcgccag cgaaggtaac
     2701 accagtttcc agtcaccctg cccggcaaac gtcgcgcatg cgcctataaa cgtccccgga
     2761 acaaatgaaa gcagcagctg tttggcctga atacacatca gaaacgcgac aataccggtt
     2821 atgacataac cgagaatttc cagatgtggt gccagtgcac taccgtaaat aatgaccatc
     2881 gcccacacca cgccactaag cagggttgca gcggagatcg ccagcccttt cagcccacct
     2941 tgcgggcagg caaagtaggc cgtacagccc aggaagcccg cccagcttag taagcccagg
     3001 gaaacagcca cccatcccca gataccggag agaatgcctg ttgtgattgc aatagagaga
     3061 agtatgttca tgccgcgcat gatagcagaa aacgcggcca tgaatgagat cgagcacaca
     3121 ttttatgcaa caacaaatta tctgttgcag ttatgagtga cgatgatcac atttactctt
     3181 cagaatcttc ttccagctcg tcccacattg cactaatggc atcacgggtt agcggtgcca
     3241 tggtgcgcca gaaaggcgtg gttgcatggg cctccacttt gccgaggaac gcaccacacc
     3301 agggtaacag atactcactg aacagtgttt ccagtgcttc gctctcatct tccgttgact
     3361 gatcttccag ccaggaagcc gcaagcagca atgtgccgat gtgatcggct ggcgtatccg
     3421 ccagtggcat ccctcgttcg gaaagaaaag cgcgcacttc cgcttccgtt gcgtcctcaa
     3481 cccatgcgct acgatatggc ggcacggcac attcatcgcc gataaacaac gcattgtaat
     3541 cggcagagac ttgcgccata tcacagcttt tctgtaaacg cgtcagtaac tcatcctgct
     3601 ccagtggcca gttcgcagcc agtttcccct cacgaatcag ggtaaacagc ggcaccagta
     3661 aaggatcttg cggttggcgg taatagagcg aacccagcac acgacagagg atagaaaact
     3721 cgttcattaa tatatttcca ttactagtaa ttaaaggtct gcaaattccg caatcggtgc
     3781 cataccgcga gattcaagga agttcagtaa gcggcgcggc gaaacattca aaatgcgctc
     3841 tggcggaaaa tctaccgcgt cgaggatttt aagacactct tcaaattccc ccatggtaaa
     3901 cgcggtgtga gaatccgagc ctaatgccac ccaacctcca gcatcacgta ccgctgcggc
     3961 taccgcacga cagttgtctt cactgccctt acgtgagtgt aaaaatgaag aattattgat
     4021 ttccagcgcc acctgatgtt tcgcggctgc ttcagcaacg gctttcacat ctatttcata
     4081 tttgggattt ccgggatggc ttattatatg cacattgccg ctggcgatag tggcgatcat
     4141 cgcttgtgta ttggtcgctt tgtcatgtgg tgcaaaaacc ggctcatgaa aaccggcaat
     4201 aatcagatcc agcgagtcaa acattttacc gctgcaatca atttcaccat caacattttt
     4261 aatgttagct tcgatgccgc gcaggatccc taccccatca accactcgcg gccagatacg
     4321 catgttaatg aagtgccagt gatgcggagc atcttccata tccgggccat gatcggtgat
     4381 cgcaaaaagt ttaatgccct tttgtttggc ctgggcaatg taatcactca atgtgctata
     4441 tgcatgtgtg ctggcaacgg tatgcatatg aaggtcgacg ggatacataa ctctctcctg
     4501 tattcaattc ccgccaagga tagcaggaat cctgatgctt tattagtagc cgcgtgcgcg
     4561 gtcgacttgc ccgcagaccc tctccccttt ttcgagctgg gcaatggtgc gagaaatgta
     4621 ctccacagct tcagcgggac gggtaatcgc ggcgacatgt ggtgttatcg tcacgcgtgg
     4681 atgttgccag agcggacttt caggcggtaa gggttcacga ttaaaaacat ccaacattgc
     4741 gcctttaact ttgccgctat ccagcgccgc gagcaggtca tcttccacaa catgaacacc
     4801 acgcgccagg ttgaggagat acgcgccatc cggtaatttt tcgagtaatt gttgattaat
     4861 aatgccgacg gtttcagggg tattcggtaa caaattaatc aatacccgac attggctcag
     4921 aaatgcagac agttcttccc gtccggcaaa gctttgcacg ccaggccacg atttacgggt
     4981 tcgactccag caacgcaacg gaaagcgcca ggtttgcaga ctctgagcaa ctttactgcc
     5041 cagtacgcct gcgcccaaaa tgccgatggt aaaatcttcc cgatgatatt caggcagcgg
     5101 ttgccaatgc gaactatttt gctggatgcg ataatcgtca aaacgtcgaa accaatgcag
     5161 cacctgactg acagcatatt cctgcatttg ctcgcccata ccggtatctt ccaggcgaaa
     5221 aagtggaaca gaagggttca gcatttcagg gtgtgcctgt agcttgctca aaatagaatc
     5281 aacaccggcc ccgagtgcga acaccgcttt aagatcgcgc cctgccagca tttcaacagg
     5341 aggatgccag actaaagcat aatcagcaga gtcattatct ccgcttttcc atgctctgac
     5401 tcttgcctga ggaatagctt tgcgcagtgc ctcaatccac cattgggtat cgaacgttgg
     5461 gtgataaaag atgatatcca tactgactcc cgaaaagcgt tgtgcgaaat ttattcgcac
     5521 ttatcgttat gatctacaaa ggccaccagc ataacaaatc cgtggtcggt ggcaaaaaaa
     5581 gcagatttcg cttattaaaa ccacacattg attgaagttt gaataaacgc gcgatttttt
     5641 caaaaagttt gttgacctca ggtcatgatt tccctaaatt agcgcccgtt ccagcaagac
     5701 aggaacgaca atttggtgag gtgtccgagt ggctgaagga gcacgcctgg aaagtgtgta
     5761 tacggcaacg tatcgggggt tc
//
LOCUS       DAPHDW010000206         5738 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.209, whole genome shotgun sequence.
ACCESSION   DAPHDW010000206 DAPHDW010000000
VERSION     DAPHDW010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5738
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.209"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            135..1415
                     /locus_tag="RH057_004222"
     CDS             135..1415
                     /locus_tag="RH057_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000821923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HDV3130503.1"
                     /translation="MEIRVNRILPLYLLLSVHSALASPLSTLLKDALLHDPSVMAARA
                     ESASARSRVEQARSAHWPVVTATGSKLLSQSHRYSYDYDSEDIFPGIRGEMNIFASGA
                     IEADVRRSESEAEYYYFKEKETGEETIRSFVSLYLDALREKQSIAVLEQSLSRHDAIL
                     NDLNTISIHDTGRESEFVQAEARRLMVRQQINTRTRVLKTTLGRLSTWTTNPVTESDL
                     ENPFSRMTESRLLTDYTQAPQKGNPSWLASSADVESKKAALKAQERARYPRVDLTGSV
                     TRDDRQIGISLSWDLFNRNASYGVSEKAAQIAAATGRLDSVARMIDETGRLSLITVRQ
                     SRMEMETLRRQEQASARVVDFYRLQFRVARKTLIELLNAENELYSVGLSRVQTEDQML
                     HGMLDYLYSQGMLLKWSGVNLSGAEEKVTGQKDR"
     gene            1420..3567
                     /locus_tag="RH057_004223"
     CDS             1420..3567
                     /locus_tag="RH057_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I secretion system permease/ATPase"
                     /protein_id="HDV3130504.1"
                     /translation="MKSIFEGLSQVTALLGTPVSAETLAAGTVRTDVSGIDFRSVGEF
                     LRSEGFDNHLSRRAPEDIPSLAVPVLLLLNAQEAIVVVRIEGAGLARQYHVLQPGGLR
                     KTLSHDELMAVYSGYCWFVQPQATQDRRSELPEYDMTGAWFWRVLWRFRPYYGQVILA
                     SFIINFLALVSSLYVMNVYDRVIPNRSWETLWMLSIGVIAAILFECAAKLIRSYLTDI
                     AGKKADLIISSALLRRVMAVKMSDRPASSGSYASNLREYESVREFMTSATLLAFVDLP
                     FILLFIGVIGLVGGWLAVVPLTLVPLVILAGILLQRPLSRHINASMRESSQRQGLLVE
                     ALDGIETLKLNNAVNWVQRRWDGYTASVARSSIRVKNTSNVIICFSTAVQQLNTVLLV
                     ILGTYLIHSDDPHNRITMGALIAAVILSGRALAPLGQIASLATRFQQARLAMQGVNDI
                     VRRPVERESQTHYLTVKDIQGEFRFSDVSFSYKPGLPDALCGLSLCIRPGERVGIIGS
                     IGCGKSTLLKMMVGLYRQNRGQITLDGVAMQQLDPCFVRDKVFLLEQNPRLFLGTLRE
                     NLQLSRLPGSLSDVDMINAAGRFGFDRFIRHHPCGLDMQIGEGGQGLSGGQRQMVALT
                     RMVLGSPRVVLLDEPTTGLDLQTENIVLKGLNHWSRGRTMVVVTHRLQVLNIVDRIIV
                     MNEGRVMMDGPRDEILKKLSGKQQSGESRAITS"
     gene            3628..4875
                     /locus_tag="RH057_004224"
     CDS             3628..4875
                     /locus_tag="RH057_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001380656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family type I secretion periplasmic adaptor
                     subunit"
                     /protein_id="HDV3130505.1"
                     /translation="MTEKTERNKASSEVVRQSDLALLTDVQAALQQEKHHALFLMVIF
                     LLVLVVTFVIWAWNSPLDEVTRGQGSIIPGSREQVIQTLDPGILKTLEVREGDIVEKG
                     QVLLTLDDTRSSAMLRESEARVNNLEAVRARLRAEAYSESLTFPDDVPADLRERESTV
                     YRLRKTELAQSIAGLKQSKALLDKEIAMTRPIVREGAMSEVELLRMQRQSAELQLQMD
                     EKQNKYLTEAGAELVKTEAELAQAKENMAGRADPVERSRLRAPLRGIVKNIRVNTLGG
                     VVSAGQDIMEIIPLEDQLLIEAYINPRDVAYVRTGMPALVKLTAYDYAIYGGLDGVVT
                     LVSPDTLRDQKRPGDLKLDPNEAYYRVLVTTSNNYLTDRNGKKLPVIPGMIASVDIKT
                     GRKTVFQYLIKPITRMKQALQER"
     gene            5164..5628
                     /locus_tag="RH057_004225"
                     /pseudo
     CDS             5164..5628
                     /locus_tag="RH057_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000781922.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1470 a         1184 c         1616 g         1468 t
ORIGIN      
        1 ttcaatgaat tctatttctg ttcgaggatc agaatggcgg tagcgtagct gaattcctgt
       61 gatgttatgt tgtctatttt cctcaattta cctcccggtt acatcgttat gtctgattat
      121 attgactgtt acttatggag attcgggtga acaggatatt acctttatat cttctgcttt
      181 ctgttcatag cgctctggct tctccattgt ctactttact gaaggatgct cttcttcatg
      241 atccatctgt catggccgcc agggctgagt ctgcctctgc acgttccaga gtggaacagg
      301 ctcgttctgc acactggccg gttgtcacgg caacgggaag taaactcctt tcacagagtc
      361 accgttattc ctacgattac gactctgaag atattttccc cggtattcgt ggtgaaatga
      421 atatatttgc ttctggagct attgaggctg atgtgcgtcg gagtgaatca gaagctgaat
      481 attattattt taaagagaag gaaacagggg aagaaacaat tcgctctttt gtttcattgt
      541 atcttgatgc gctaagggaa aaacaatcca ttgcggtgct tgaacagagt ctctcacggc
      601 atgacgcaat tcttaatgat cttaatacta tcagtattca tgataccggg cgggagtctg
      661 aatttgttca ggccgaagca agaaggttga tggtcaggca gcagataaat acccgcacca
      721 gagtgctcaa aaccacgctg ggaagacttt cgacctggac tacaaatccg gtaaccgaat
      781 ccgatcttga aaatcctttt tcccggatga cagagtccag attgttaact gattatacgc
      841 aggcgcctca gaaagggaat ccgtcgtggc ttgccagcag tgctgatgtt gagagtaaaa
      901 aagcggcgct gaaagcgcag gagcgtgccc ggtatccccg ggtggattta acgggatctg
      961 taacccggga tgacaggcag ataggtatca gtctgtcctg ggacctgttt aaccgtaatg
     1021 ccagttatgg tgttagtgaa aaagctgcgc agatagctgc agcgacgggg cgactggact
     1081 cagtcgcccg aatgattgat gaaaccggac gattatctct gataacggtc agacaaagtc
     1141 ggatggaaat ggaaacactc agacgtcagg agcaggcctc tgccagggtt gtggactttt
     1201 atcgtcttca gttccgggtg gcaagaaaaa cactgattga attactcaat gcagaaaacg
     1261 aactgtacag cgtcggactc tcccgggttc agacggagga tcagatgctc cacggaatgc
     1321 tggattatct gtattcccag ggaatgctcc tgaaatggag cggagtgaat ctttctggtg
     1381 cagaagaaaa agtaacaggg cagaaggaca gataaataag tgaagagcat ttttgaagga
     1441 ttatcacagg taacggcatt actgggtaca cctgtatctg ctgagacgct ggctgccggt
     1501 actgtccgta cagatgtctc cgggattgat tttcgttctg ttggcgagtt tcttcgcagt
     1561 gaagggtttg ataatcatct ttcccggcgg gctccggaag atattccttc acttgcggtt
     1621 cctgtgcttc ttttactgaa tgcacaggaa gccattgtgg tggtgcgtat tgagggtgcc
     1681 ggactggccc ggcagtatca tgtactccag cccgggggac tccgtaaaac actttctcac
     1741 gatgaattaa tggctgttta ttcaggttac tgctggtttg ttcagccaca ggcaacacag
     1801 gatcgtcgtt cggagctgcc ggagtatgac atgaccgggg catggttctg gcgggtactg
     1861 tggcggttca ggccatatta cggacaggtc attcttgcga gttttatcat taactttctt
     1921 gccctggtca gttccctgta tgtcatgaac gtttatgacc gggtcatacc caatcgctcc
     1981 tgggaaacgt tatggatgct gagtatcggg gtgattgcag ccattctgtt tgagtgtgct
     2041 gcaaagttga tccggagcta tctgacggat attgcgggta agaaggcaga cctgattatc
     2101 agctcagcac tgttacggcg ggtgatggca gtgaaaatgt cggacaggcc ggcgtcttcc
     2161 ggttcatacg ccagcaattt gcgggaatat gagtctgtca gggagtttat gaccagcgcc
     2221 acgctgctgg cttttgtgga tttgccgttt atcctgcttt tcatcggtgt catcgggctt
     2281 gtggggggct ggcttgctgt tgttcctctt acactggttc cgctggtgat tctggcagga
     2341 atattactgc agcgaccgct ttcccgtcat attaatgcct ccatgcggga atcatcgcag
     2401 cgccagggat tacttgtgga ggctctggac ggaatagaaa ccctgaagct gaataatgca
     2461 gttaactggg tccagcgccg ttgggatgga tatacagcca gtgtcgcccg ctcgtccata
     2521 cgggtaaaaa ataccagtaa tgtcataatc tgcttttcca cagcagttca gcaactgaac
     2581 actgtgctgc tggtcattct gggaacgtac cttattcaca gtgatgaccc acacaaccga
     2641 atcaccatgg gggcactgat tgccgcagtt attctttccg gtcgtgcgct ggcaccgcta
     2701 ggtcagatag catcactggc cacccgtttt cagcaggccc gcctggccat gcagggagtg
     2761 aatgacattg tccgccgtcc ggtggagcgg gaaagtcaga cacattatct gacagtgaag
     2821 gatatccagg gagagtttcg tttcagtgat gtctcctttt cttataagcc cggattaccc
     2881 gacgctttgt gcggtctgtc actctgtatc cggcccggag agcgggtggg tattattggc
     2941 tcaataggtt gtggtaaatc gacgctgctg aaaatgatgg tggggctgta ccgtcagaac
     3001 agggggcaaa ttactcttga cggggttgcg atgcagcaac tggacccctg ttttgtccgg
     3061 gataaagtgt ttcttctgga acagaatccc cggctttttc tgggaactct gcgtgaaaat
     3121 ctgcagcttt cgcgtcttcc gggaagcctg tcggatgtgg atatgattaa tgcagccgga
     3181 cgtttcgggt ttgaccgctt tattcgtcat cacccatgcg ggcttgatat gcagataggg
     3241 gaaggaggtc aggggttgtc aggaggacag cgtcagatgg tcgctctgac gagaatggta
     3301 ctgggttctc cccgggtagt tctgcttgat gaacccacga cgggtcttga tctgcagaca
     3361 gagaacattg ttctgaaagg gctgaatcac tggagccgtg gacgcacaat ggtggtggtg
     3421 acgcatcgtc tgcaggtgct gaatattgtt gaccgtatta tcgtgatgaa tgaaggccgg
     3481 gtaatgatgg atggtccccg cgatgaaata ctgaaaaaac tgtcagggaa acagcagagt
     3541 ggggagagca gagcgataac ttcatgagcg tcaaccggat aagaaataaa gagcatatat
     3601 gtttaacagt ttgtggaccg caggggtatg acagaaaaaa cggaacggaa taaagcgtcg
     3661 tcagaggtgg tcaggcagtc agacctggcc ctgttgacag atgtacaggc agcccttcag
     3721 caggaaaaac atcatgccct tttcctgatg gtgatttttc tgctggtact ggtagtgacg
     3781 tttgttatct gggcatggaa cagtcctctg gatgaagtga cccgtggtca ggggagtatc
     3841 attcccggca gcagggagca ggttatacag acccttgacc cgggtatttt gaaaacactt
     3901 gaggtcaggg aaggagatat tgttgagaaa ggtcaggtac tgctgacact ggatgatacc
     3961 cgaagttctg ccatgctgag ggaaagtgaa gcccgggtga ataacctgga agcagtcagg
     4021 gcaagactcc gtgcagaagc ttattccgaa tcactgacat ttccggatga cgtgcctgca
     4081 gatttgaggg agagggaaag tactgtttat cgactacgaa agactgaact ggcacaaagc
     4141 atcgccggcc ttaagcagag taaggctctg ctggataagg aaatagcaat gacccggcca
     4201 attgtgcgtg aaggggcaat gtcggaggtc gaactgctac gtatgcagcg tcagtcagcg
     4261 gaactgcagt tacagatgga tgagaaacag aataaatacc tgacggaagc cggtgctgaa
     4321 ctggtgaaga cagaagcaga gcttgctcag gcaaaagaga atatggctgg cagagcagac
     4381 ccggttgagc gttcacgtct cagggcaccc ctgagaggta tcgtaaaaaa tatacgggta
     4441 aacactctgg gaggggtggt ttctgcagga caggacataa tggaaattat tccgctggaa
     4501 gaccagttgc tgattgaagc ttatataaat ccacgggatg ttgcctatgt cagaacaggg
     4561 atgccggcac ttgtaaaact gacggcttat gactatgcca tttatggtgg tcttgacggt
     4621 gtggtgacac tggtaagtcc ggatacttta cgtgaccaga aacgtccggg agacttaaaa
     4681 ctggacccga atgaagctta ttaccgggta ctggttacaa ccagtaataa ttatctgaca
     4741 gacaggaatg ggaaaaaatt accggttatt cctggcatga tagccagcgt ggatataaaa
     4801 accgggcgta aaacggtatt tcagtatctg attaaaccga taaccagaat gaagcaggca
     4861 cttcaggaaa gataataaaa aggtcttccg ggaaaaatat tttaaatcaa ttttaaacgg
     4921 gcagaatgat ttcactttat tctgcccggg tcattgcgca tgcctgtatg taatgtgtgg
     4981 ctgaagataa tacaagggtt atatctgtca ggggaaaata aatcctaaaa aagcagaatg
     5041 attaaggagg gccagccaga gaaaataaat tatctgtaat ctgatttgtt tttatttaat
     5101 gactcccttt agtgcggata aaacataaga gcatacttgt tttattaacc tgaggatact
     5161 tatatgaaaa tgattttcac cggcaaggtc agtggagaaa aaacagtact ggcagccgga
     5221 gcccgtcata ctgtaaaggc acaggccggg gaacagtatg gactggtcga tgaggtgaca
     5281 ggtctggttc cggatggtgt ggaagcggac cgtacaggcg atgatttaat tctgcgtaaa
     5341 aaggaagatg atacagaaat ccggattgag ggattctggg aaaaatgcca gcctggtgaa
     5401 acacagtgta cagccgtgtt taatgttgta ggtgaaaatg gtcaggttac tgaagctgtc
     5461 ttgacccagg atggtcctgt actggagaat tttactgcag gacaaagtgg cacactggct
     5521 gaaggtatca gtaacgggat tatctggggc gggattgcat ttggtgcggg actggcggct
     5581 attgctctgc ttgctggagg tggtggcgga agcagtcgcc ataaaagtgc tgctggagag
     5641 gggcagaatc aggtacaggc tgaaggcagt caggaaactg ggaaaattga tggacctccg
     5701 acagtctcta cgaaaccggt aaatgggaat tctttcgg
//
LOCUS       DAPHDW010000207         5569 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.210, whole genome shotgun sequence.
ACCESSION   DAPHDW010000207 DAPHDW010000000
VERSION     DAPHDW010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.210"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            254..2686
                     /locus_tag="RH057_004226"
     CDS             254..2686
                     /locus_tag="RH057_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HDV3130506.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPEQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPDSDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPLPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVRKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2753..4222
                     /locus_tag="RH057_004227"
     CDS             2753..4222
                     /locus_tag="RH057_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HDV3130507.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4222..5403
                     /locus_tag="RH057_004228"
                     /pseudo
     CDS             4222..5403
                     /locus_tag="RH057_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110071.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
BASE COUNT         1652 a         1345 c         1345 g         1227 t
ORIGIN      
        1 tatttataag ggagaagtat aaaaccaagg agtagataaa ttagcagaat gacatctaat
       61 gccttagtac aaaaatattg tttcactcct tgcaagaacc gaacattctc catatgaatc
      121 cttatcctac tattgatgaa actcaagaat acctttaaaa cagtgactta acgctgataa
      181 tactctttga acagatgaat tcatctattt ttctgtgtag aatttgcgca tcgcatttag
      241 atgagaacac cccatggcgg aactgaacct aagtaacctg acggaagcag acatcattac
      301 caaatgcgtt atgccagcca ttctcaatgc aggctgggac aacacaacgc aaatcagaca
      361 ggaggtcaaa ctccgggacg gtaaagtcat cgtacgtggt aaagttgcgg cacgcagaac
      421 ggtaaaatct gctgacatcg tgctgtatca caaaccaggc attcccttag ccgtgattga
      481 agcaaaagcc aacaaacatg aaattggcaa agggatgcaa cagggcattg aatatgcgcg
      541 cctgctggac gttccttttg tttttgccac caacggtgat ggctttatct tccgcgatgc
      601 cacggcagcc gaaggtgaat gccttgaaaa gcaaataacg ctggatgact tcccctcccc
      661 cgctgaactc tggcaaaaat tctgcctctg gaaaggatac acacaagctc agcttccggt
      721 gattactcag gattattacg acgatggcag cggtaaatcg ccacgttatt accagcttca
      781 ggcaatcaac aaaaccattg aagccgtctc caacgggcaa aaccgcgttc tgctggtcat
      841 ggcgaccgga acagggaaaa cctataccgc attccagatc atctggcgcc tgtggaaatc
      901 aaaaaataaa aaacgcattt tgttccttgc cgatcgcaac attctggtcg accaaaccaa
      961 aaataatgat ttccagccat ttggtacggc aatgaccaaa gtcagcggac gcaccattga
     1021 tcccgcttat gaaattcacc tcgcgctcta tcaggctata actggcccgg aggaagacca
     1081 aaaagcgttt aaacaagtcg caccagattt cttcgatctg atcgtgatcg acgaatgcca
     1141 tcgcggcagc gcatccgaag acagcgcctg gcgagagatc cttgattatt tcagttccgc
     1201 cacccaaatt ggcttaaccg ccacgccaaa agagacgcat gaagtctcca gcacggatta
     1261 cttcggcgat ccggtttacg tctactcgct aaaagaaggg atcgaagacg gcttcctcgc
     1321 cccttataaa gttgtccgtg ttgatattga tgttgatctg caaggctggc gcccaaccaa
     1381 agggcaaact gacttaaacg gcgaagtgat cgacgatcgt atctataacc agaaagattt
     1441 cgatcgcacg atggtaatcg acgaacgcac agaactggtt gccagaacca ttaccgacta
     1501 cctcaagcgc accaatccga tggataaaac catcgtcttc tgtaacgaca tcgatcatgc
     1561 agaacgtatg cgccgcgccc tggttaatct caacccggag caggtgaaaa agaacgacaa
     1621 atacgtcatg aaaatcaccg gcgatgatga aattggcaaa gctcagttgg ataacttcat
     1681 caacccgaaa aaaccgtacc cggttatcgc gaccacttca gagctgatga ccaccggtgt
     1741 ggatgctaaa acctgcaaac tggtagtact ggaccagaac atccagtcga tgaccaaatt
     1801 caagcagatt atcggtcgtg gtacacgcat cgacgaacgt tacggcaaac tctggtttac
     1861 catcctcgac tttaaaaaag ccaccgaact gtttgccgat gagcgtttcg atggcattcc
     1921 cgaaaaagtc atggatacca caccagagga tatcgccgat ccagactctg attttgaaga
     1981 gaaactcgaa gaaatcagcg aacatgacga cgaacaggta acaggcgttg atgaaccgcc
     2041 tgcgccacca taccaggtta ccgataccga tgatgtcggc ccacttccgg aagaagacga
     2101 gaagaaaatc cgcaagtttc acgtcaacgg tgtagcagtg ggcgttattg cgcagcgtgt
     2161 tcagtattac gacgccgacg gtaaactggt taccgaatcc tttaaagatt acacccgcaa
     2221 aacactgctc aaagaatatg cctcgctgga tgactttacc cgcaagtggc aggacgccga
     2281 tcgcaaagaa gcgatcattc acgagctgga gcaacagggg atcatctggg aagtactggc
     2341 agaagaagtc ggtaaagatc tcgacccatt cgacatgctt tgccacgtag tgtatggtca
     2401 gccgccgtta acccgcaaag agcgcgccga gaacgtgcgc aagcggaact acttcacaaa
     2461 atactctgaa gcagcgcaag ccgtgctcga taatctgctg gataaatacg ccgatgcggg
     2521 cgtacaggag atcgaaagta ttcaggtgct gaaacttaag ccattcgaca gcatgggcac
     2581 cttaccggag attattaaaa ccggatttgg cgaccgtaac gggtataatc aggcgctcag
     2641 cgagctggaa aacgaaatct accaattacc gccccgctct gcttaacaac tctcttttgc
     2701 gccttctgca accatcatgt tgcagaaggc cttgtttttc atggaataca acatgtctat
     2761 cagttcagta atcaaatcat tacaagatat tatgcgcaaa gatgccggtg tggatggcga
     2821 tgcgcagcgt ctcggtcagc tctcctggct gctatttttg aaaatcttcg atgcccagga
     2881 agaggcgctg gaactggagc aggataacta tcaatatccg atcccacagc gttatttgtg
     2941 gcgcagttgg gccgccaacg cgcagggcat taccggtgat tccctgctgg aattcgttaa
     3001 tgatgatctg ttcccggcgt tgaaaaacct cactgcgcct atcgataaaa acccacgcgg
     3061 ctacgtggtg aagcaggcgt tcagcgatgc ctataactat atgaaaaacg gtacgctact
     3121 gcgtcaggtg atcaacaagc tgaacgaaat tgactttacc agcgccagcg aacgccatct
     3181 gtttggtgat atttacgaac agatccttaa agatctgcaa tctgcgggca atgctggcga
     3241 attctatact ccacgcgccg tcactcgctt tatggtggat cgcgttgatc cgaaactcgg
     3301 cgaatccatt atggacccgg cctgcggtac gggcggtttt cttgcctgcg cgttcgatca
     3361 tgtaaagaac aaatacgtga agagcgtcgc cgatcatcag acgctgcaac aacagatcca
     3421 cggtgtcgag aaaaaacagc ttccgcacct gctggcgacc accaatatgc tgctacacgg
     3481 catcgaagta ccggtacaaa ttcgtcacga caacaccctg aacaaaccgc tttcctcctg
     3541 ggatgagcaa ctggatgtca ttgttaccaa cccgccgttt ggtggaacgg aagaagacgg
     3601 tattgagaag aactttccgg cagagatgca aacccgcgaa acggcggatc tgttcctgca
     3661 actgattgtg gaagtactgg cgaaaaacgg tcgcgcggcg gtggtattgc cggatggcac
     3721 gctatttggc gaaggcgtta aaaccaaaat caaaaagcta cttaccgaag agtgcaacct
     3781 gcatactatc gtgcgtttac cgaatggggt gtttaacccc tataccggca ttaaaaccaa
     3841 cctgttgttc tttaccaaag gtcagccaac caaagagatt tggttctatg agcatccgta
     3901 tccggcgggc gtgaaaaact acagcaaaac caagccgatg aagtttgaag agtttcaggc
     3961 ggagattgac tggtggggta acgaggccga tggttttgcc agccgcgtcg agaatgagca
     4021 ggcatggaaa gtcagcattg atgacgtgat tgcgcgtaac ttcaatctgg atattaaaaa
     4081 cccacatcag gcggaaaccg tcagccatga tccggacgag ctgttagcgc agtatgcaaa
     4141 acagcaggcg gagatccaga cgctgcgtaa tcaactgcgc gatattcttg gcgctgcgct
     4201 gtctggcaag gaggttaact aatgagtgtg gaaaagctga tcgttgatca tatggaaacc
     4261 tggacctcgg cgttgcaaac ccgttccacc gccgggcgcg gcagttccgg aaaaattgat
     4321 ttatatggca ttaagaaatt acgtgagctg attctggaac tggctgtgcg cggtaaactg
     4381 gtgccgcagg acccgaacga tgaaccggcg tcggagctgc tgaagcgtat tgcggcggaa
     4441 aaagcggagc tggtgaagca aggtaaaatt aaaaagcaaa aaccgctgcc ggaaattagc
     4501 gaggaagaga agccgtttga attgccggag ggatgggagt gggtcacact ggcaaccgta
     4561 ggtgaaatag tcggaggagg aactccaaag tccgataatc cgcaattttg ggccaaaaat
     4621 ggtattaagt ggattactcc agcagacctc tacggactca aagggaagta cattacatct
     4681 ggagcccgtg atatttcacc cgcaggactg tcaaactcat cagcccggtt gatgccaaag
     4741 gggagtgtgc ttttctctag ccgagcacca attggatatg tcgctatagc agatgcagaa
     4801 ctgtcaacaa accagggttt taaatcttgt gttccatata tcaaagaaag tgcagaatat
     4861 atatattact ttctactggc atctgcaaaa aaaatagatg cagaggcaag tgggactaca
     4921 tttaaagaag tctctggggc aattgtttcg aaaatattat tacctttacc gccattgagt
     4981 gaacaactaa aaatcgtctc aagggcaaat gaacttatgt ccctatgcga ccaactggaa
     5041 cagcaatccc tgacaagtct ggacgcacat cagcaactgg ttgaaaccct gttgggaaca
     5101 cttacagaca gccaaaacgc cgaggaactg gctgaaaact ggacgcgtat tagcgagcat
     5161 ttcgacacac tatttaccac cgaagccagc gtggatgcgt taaaacagac cattctgcaa
     5221 ctggccgtaa tgggtaaact tgtgccgcag gatccgaatg acgaaccagc ctccgaactg
     5281 ctcaaacgta ttgcgcagga aaaagctcaa ctggtgaaag aagggaaaat aaaaaaacaa
     5341 aaatcgttgc caccgattag cgatgaggaa aaaccgtttg aacttccgga agggtgggag
     5401 tggagttacc tatcagatat tggcatactt gctagaggaa gatctaaaca tagacctcgt
     5461 aacgatccca ctctttatgc tgatggcaca atcccattag tacaaacagg tgatgttgca
     5521 agatctaatg gatgtataaa tacatattcc gcgctatata atcaactag
//
LOCUS       DAPHDW010000208         5473 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.211, whole genome shotgun sequence.
ACCESSION   DAPHDW010000208 DAPHDW010000000
VERSION     DAPHDW010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5473
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.211"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            220..492
                     /locus_tag="RH057_004229"
     CDS             220..492
                     /locus_tag="RH057_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130508.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYQSVFTDFIAGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
     gene            473..1681
                     /locus_tag="RH057_004230"
     CDS             473..1681
                     /locus_tag="RH057_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HDV3130509.1"
                     /translation="MTRSGGDFQPRPLKRLFTANQCWTSFLDAGGLRDIEVEAVTKML
                     ACGTRILGVKEYNCDKPECPHVRYVTNSCGSRACPSCGKKATDLWIATQLNRLPDCDW
                     VHLVFTLPDTLWPVFESNRWLLNDVCRLAVENLLYAARKRGLEPGIFCAIHTYGRRLN
                     WHPHVHVSVTCGGLNKHGHWKKLSFLKDAMRSRWMWNMRQLLLKAWSEGLAMPESLSH
                     ITTESQWRSLVLKAGGKYWHVYMSKKTAGGRNTARYLGRYLKKPPIAASRLAHYNGGA
                     SLSFRYLDHKTGETATETLTQRELVARLKQHIPEKFFKMVRYFGFLANRVCGEKLPQV
                     YRALGMDKPEPVAKVCYAQMVKQFLSRDPFECVLCGGRMVYRRAIAGLNVSGLKKNAR
                     DISLLRYMPA"
     gene            complement(1971..2075)
                     /locus_tag="RH057_004231"
                     /pseudo
     CDS             complement(1971..2075)
                     /locus_tag="RH057_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
     gene            complement(2077..2400)
                     /locus_tag="RH057_004232"
                     /pseudo
     CDS             complement(2077..2400)
                     /locus_tag="RH057_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            3143..4120
                     /locus_tag="RH057_004233"
     CDS             3143..4120
                     /locus_tag="RH057_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HDV3130510.1"
                     /translation="MDKSSGELVTLTPNNNNTVQPVALMRLGVFVPTLKSLKNSKKNT
                     LSRTDATEELTRLSLARAEGFDKVEITGPRLDMDNDFKTWVGIIHSFARHNVIGDKVE
                     LPFVEFAKLCGIPSSQSSRRLRERISPSLKRIAGTVISFSRTDEKHTREYITHLVQSA
                     YYDTERDIVQLQADPRLFELYQFDRKVLLQLKAINALKRRESAQALYTFIESLPRDPA
                     PISLARLRARLNLKSPVFSQNQTVRRAMEQLREIGYLDYTEIQRGRTKLFCIHYRRPR
                     LKAPNDESKENPLPPSPAEKVSPEMAEKLALLEKLGITLDDLEKLFKSR"
     gene            complement(4399..5139)
                     /locus_tag="RH057_004234"
     CDS             complement(4399..5139)
                     /locus_tag="RH057_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3130511.1"
                     /translation="MNNVIPLQNSPERVSLLPIAPGVDFATALSLRRMATSTGATPAY
                     LLAPEVSALLFYMPDQRHHMLFATLWNTGMRIGEARMLTPESFDLDGVRPFVRILSEK
                     VRARRGRPPKDEVRLVPLTDISYVRQMESWMITTRPRRREPLWAVTDETMRNWLKQAV
                     RRAEADGVHFSIPVTPHTFRHSYIMHMLYHRQPRKVIQALAGHRDPRSMEVYTRVFAL
                     DMAATLAVPFTGDGRDAAEILRTLPPLK"
     gene            complement(5260..5448)
                     /locus_tag="RH057_004235"
     CDS             complement(5260..5448)
                     /locus_tag="RH057_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001309252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HDV3130512.1"
                     /translation="MASDTFSCRRRLSAGFFSVSACSWHYPNSPKNGKPVLSPHHDWL
                     KTGSLRQNAPLRIWFYPQ"
BASE COUNT         1337 a         1373 c         1449 g         1314 t
ORIGIN      
        1 tttatatcct ttgactacat gcaagataag ggaaggttct atttattgac attaataccc
       61 tcgacaattg ctgagcagaa gcccccacga caagtgacag aatgacaaca tctacagtgg
      121 ggagcgtttt atcgatgaag aaccacggca gcctggcggc tgcaaatcgt ggtgtggcag
      181 aatatgagct ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac
      241 cagaaagtcc acaccagaat ggtgaaagag gttctgaaag ccgtcgccag ggccaataat
      301 cacccttatc agtctgtatt tacagacttt atcgccggtc atccctcctg tacggtctgt
      361 ttctgggaaa ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc
      421 catacctgcc gtcgttttga tttatacgaa actgaagcgg agatgaaggc ggatgacccg
      481 aagtggtggt gattttcagc cccgaccgct gaaacgcctg ttcacagcca accagtgctg
      541 gacatccttc ctggatgcgg gcggcctgcg cgatatcgag gttgaggctg tcacaaaaat
      601 gctggcctgc ggcacgcgga tactgggtgt gaaggaatac aactgcgata aacctgagtg
      661 cccccacgtc agatacgtca ctaactcatg tggcagccgt gcctgcccgt cctgcggaaa
      721 aaaggccaca gacctgtgga tagcgacaca gctgaatcgt cttcctgact gcgactgggt
      781 gcacctggtc ttcaccctgc cggacacgct gtggccggtg ttcgaaagca accgctggct
      841 gctgaatgac gtgtgccgtc tggcggtgga gaatctgctg tatgcagccc ggaaacgggg
      901 tctggaaccc ggtatcttct gcgccatcca tacgtatggc cgtcgtctca actggcatcc
      961 gcatgtgcat gtgtcagtga cctgcggagg tctgaataaa cacggacact ggaaaaagct
     1021 gagcttcctg aaagacgcga tgcgttcgcg gtggatgtgg aatatgcggc agctgcttct
     1081 gaaagcgtgg tcagaaggtc tggcgatgcc ggaatcgttg tcacatatca cgacggaatc
     1141 acagtggaga agcctggtgc tgaaagccgg cggaaaatac tggcatgtgt acatgtcgaa
     1201 gaagacagcc ggagggcgga atacggcacg ctacctgggt cgttatctga agaagccacc
     1261 aatagcggct tcccggctgg cgcattacaa cggaggagcg agtctgagct tccgttacct
     1321 ggaccacaaa acgggagaaa cggcgacgga aacgctgaca cagcgggagc tggtcgcgag
     1381 gctgaaacag cacatcccgg agaagttttt taagatggtg agatacttcg ggttccttgc
     1441 caaccgggtg tgtggagaga agctgccgca ggtgtaccgt gctctgggga tggataagcc
     1501 ggaaccagtg gcgaaagtgt gctatgcaca aatggtgaaa cagttcctga gtcgtgaccc
     1561 gttcgaatgc gtgctgtgtg gcggccggat ggtataccgc cgggccattg cggggctgaa
     1621 tgtatcaggg ctgaagaaaa acgcgcggga tatcagtctg ctgaggtata tgccggcctg
     1681 acacaggcta agtgtgcccg ggaatcggtg caggtataaa aaacagcctg ttacggtata
     1741 aaaatcgacc atgcagagag agaaaccacc ggagtggagg gaaaagcaga aaggctcacg
     1801 tggcataagg cgtggacagc ggtatcgtct ggtttttcaa tttcctatac gtgaacaaca
     1861 gcctggtgca tggcatcttt gtctgtgatt cgttcaccaa aactgcgact atgtcaacgc
     1921 cacgatgttt gaccgttatt tgccattttc atgcccgcta aatactttcg ttactgatgc
     1981 tgtccgcttt gcaccatttc atcatcactg attggcgttg tgtttttggt cagcactcca
     2041 gctttacgtt tctcttttaa ccggtaactc tctccttgtt ctttttgacg gacgcatttt
     2101 acgtttgggc tggatgtagt aacgcagcat ggaacgtccg cccgtataac ccattgcctt
     2161 aatctccgcg aagataacct cgctattcca gacattctct gccaggcgca tgtcgatgta
     2221 atccataaac ggtttcagct taaccatttt gtggcgggtc tttctggctg gcggttcagg
     2281 gtatttgagg tagcgtctga ccgtccgttc agagcaacca atctgagccg caatatcgac
     2341 aatgtacgcg ccctgctggc gcatttgctt tatcatgtaa aagtcctctc tgctcagcat
     2401 gttgatgtcc tttctggtgt gagaacctta aggaaacaac atgttgggtg gagcggacaa
     2461 tccaaatggt gaattaccgt cttatatcac tggcgctgac acctgtagtt tgtatacaca
     2521 atccctggca ttgaaacaca catatgaagt gtacttcgga tatgaccgta cgctcaggaa
     2581 gtacggcaga ttccgtcaaa aattacctgt aaagacagac atcagaaaaa acagtgctcg
     2641 ccgcagaccg gtgaccgggc gaagcccgcg aaccggcgag gaagcgcaat ggatctgtag
     2701 ttacagtgag caggtacgcc ctgaagcctt cataatcggt agtgtatgct gtttttctgg
     2761 aatgatttat ccacatatcc agaggcccga tccagtaaac agatccatga atgatcaaca
     2821 aaggatccat taaagatccc catatcgctg tcaaccttgt cactcatggg ccgggaccac
     2881 gttcacataa gcggtggcat gttatggata aactgtaaca ggttcatgat aagctgtatt
     2941 cagtaatgca tatactgaag taagttaatg acataaacta tggtcagtac gccagactca
     3001 gttgttaaat acagactgca ggtttttctt cagtcagttg acggggttct gacacacgat
     3061 tttctgttta ttcttttact gtccacaggc aggaggcttt ctggaaaacg aaaattcaga
     3121 catcaaaaaa ctgttcggcg aggtggataa gtcgtccggt gagctggtga cactgacacc
     3181 aaacaataac aacaccgtac aacctgtggc gctgatgcgt ctgggcgtct ttgtaccgac
     3241 ccttaagtca ctgaagaaca gtaaaaaaaa tacactgtca cgcaccgatg ccacggaaga
     3301 actgacacgt ctttccctgg cccgtgcaga aggattcgat aaggttgaga tcaccggccc
     3361 ccggctggat atggataacg atttcaagac ctgggtgggg atcattcatt cctttgcccg
     3421 ccataacgtg attggtgaca aagttgaact gccttttgtt gagtttgcaa aactgtgtgg
     3481 tataccttca agccagtcat cccgcaggct gcgtgagcgc atcagccctt ccctgaagcg
     3541 cattgccggt accgtgatct cgttttcccg taccgatgag aagcacaccc gggaatacat
     3601 cacccatctg gtacagtcag cctactacga tactgagcgg gatattgtgc agttacaggc
     3661 cgatccccgc ctgtttgaac tgtaccagtt tgacagaaaa gtccttctcc agcttaaggc
     3721 gattaatgcc ctgaagcgac gggagtccgc ccaggcactc tacaccttta tagagagcct
     3781 gccccgggat ccggcaccga tatcgctggc gcggctgcgt gcacgcctca atctgaagtc
     3841 tcctgtattt tcccagaacc agacggtcag acgggcaatg gagcagctgc gcgagattgg
     3901 atatcttgat tacacggaga tccagcgggg gcggacaaaa ctcttctgca ttcactaccg
     3961 gcgtccccgg ttaaaagcac cgaatgatga gagtaaggaa aatccgttgc caccttcacc
     4021 tgcggaaaaa gtcagtccgg agatggcgga gaagcttgcc ctgcttgaaa aactgggcat
     4081 cacgctggat gacctggaaa aactcttcaa atcccgctga acataaactg tagtcagtga
     4141 agagtgctcc tttactgact acagcttata ttatcaggtg cagtgagtgg tctgctcact
     4201 gcagtttata ttcagtttca tgcagtgctg cctgtggctg agctgccatc tgcctgtccc
     4261 ttacgtgagt caccccgtaa cctgatgctg aggcattgct cccttcataa aacatggttt
     4321 actcactaca gcttatctac ctgctccagc ttatgttatg tctgttctgc tgaccactgc
     4381 ttatataagg aggtgccgtt acttcagggg aggcagtgta cgcaggatct ccgcagcatc
     4441 ccgcccgtca cctgtgaaag gcactgccag cgtggctgcc atatccagtg caaacactct
     4501 ggtataaacc tccatagaac gtggatccct gtgaccagcc agtgcctgga tgactttccg
     4561 gggctggcgg tgatagagca tgtgcatgat atagctgtgc cggaaggtgt gtggtgtgac
     4621 cggaatcgaa aagtgtactc cgtcagcttc ggcccgtctg acagcctgct tcagccagtt
     4681 gcgcatggtt tcgtcggtca cggcccataa tggttcacga cgacggggcc gggtggtgat
     4741 catccagctt tccatctgcc tgacatagct tatatccgtc agcggaacca ggcggacttc
     4801 atcttttggc gggcgtccgc gtcgcgcacg cactttttcg gacaggatcc gcacaaacgg
     4861 tcttactcca tccaggtcaa atgattccgg tgtcagcatc cgggcttcgc caatacgcat
     4921 tccggtattc cagagggtgg cgaacagcat atggtgacgc tgatccggca tatagaaaag
     4981 aagggcactc acttccgggg ccagcaggta ggccggtgtg gcccctgtgg aagtggccat
     5041 tcttctcagg gagagcgctg ttgcaaaatc cacccccggc gcaatgggta acagggagac
     5101 gcgttctggt gaattctgca ggggaatgac attgttcatg agttattttt cacgtagcac
     5161 acgcactgtt tatggataca ggtattctga cctgaacgcc cggatccttc cacgacaata
     5221 aatcgcagcg cgcggatcct tttgtttgct tatcacgcat tattgcgggt aaaaccaaat
     5281 acgtaaaggc gcgttttgcc gcagggatcc tgtcttaagc cagtcatggt gcgggctgag
     5341 gactggtttg ccgtttttgg gagaattggg gtaatgccag gaacaggcag atacggagaa
     5401 aaatcctgca gataaccttc tacgacaact gaaggtgtct gacgccatat tcttagctac
     5461 ttctcagttt taa
//
LOCUS       DAPHDW010000209         5460 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.212, whole genome shotgun sequence.
ACCESSION   DAPHDW010000209 DAPHDW010000000
VERSION     DAPHDW010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5460
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.212"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(125..1408)
                     /gene="aroA"
                     /locus_tag="RH057_004236"
     CDS             complement(125..1408)
                     /gene="aroA"
                     /locus_tag="RH057_004236"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HDV3130513.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGVSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLGSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMTIATAALFAKGTTTLRNI
                     YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAEIATYNDHRMAMCFS
                     LVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
     gene            complement(1479..2567)
                     /gene="serC"
                     /locus_tag="RH057_004237"
     CDS             complement(1479..2567)
                     /gene="serC"
                     /locus_tag="RH057_004237"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HDV3130514.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            complement(2766..3458)
                     /locus_tag="RH057_004238"
     CDS             complement(2766..3458)
                     /locus_tag="RH057_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HDV3130515.1"
                     /translation="MKAFDLHRMAFDKVPFDFLGEVALRSLYTFVLVFLFLKMTGRRG
                     VRQMSLFEVLIILTLGSAAGDVAFYDDVPMVPVLIVFITLALLYRLVMWLMAHSEKLE
                     DLLEGKPVVIIEDGELAWSKLNNSNMTEFEFFMELRLRGVEQLGQVRLAILETNGQIS
                     VYFFEDDKVKPGLLILPSDCTQRYKVVPESADYACIRCSEIIHMKAGEKQLCPRCANP
                     EWTKASRAKRVT"
     gene            3588..5348
                     /locus_tag="RH057_004239"
     CDS             3588..5348
                     /locus_tag="RH057_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     accessory protein YcaO"
                     /protein_id="HDV3130516.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLTENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLEAEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFKKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
BASE COUNT         1414 a         1409 c         1281 g         1356 t
ORIGIN      
        1 caacttacat tgacaagcga cgctaccatt aatcaaaatg actcacaagg tccgaaaaaa
       61 aacgccgaca atgtgccgac gttttgataa agaaagattt ggctatttat tgcccgttgt
      121 tcattcaggc tgcctggcta atccgcgcca actgctcgaa ataatccgga aatgttttgg
      181 ccgtgcattt gggatcaaga atcgtcactg gtgtatctga caacgccacc agcgagaaac
      241 acatcgccat ccggtgatca ttgtatgtcg cgatctcggc aaagttcagt ttttccggag
      301 gagtgatacg aatgtaatcg tgcccctctt ccacttccgc gccgacttta cgcagttctg
      361 ttgccatcgc aaacaggcga tcggtctctt taacacgcca gttatagata ttgcgcagcg
      421 tggtggtgcc ttttgcaaat aacgccgccg tggcaatggt catcgccgca tcaggaatat
      481 ggttcatatc catatcaata gcgttcagtt caccacgcgt gcaggaaata taatcatcgc
      541 cccagcaaat ggtcgcgccc attttttcca gcacatcagc aaagcgaata tcaccctgca
      601 tactgttacg tccaataccg gtcactttta cagtgccgcc tttgattgct gctgctgcca
      661 gaaagtaaga agccgaagat gcatcgcctt cgaccaaata agtacccgga gactgataag
      721 actgcccgcc ttttacgaca aattgttgat agtgctgatt ttcaatttca acaccaaacg
      781 tcttcatcag attgagtgtg atgtcgatat aaggtttaga aaccagatcg cctttaatac
      841 gaatcaccgt atcttccggc gcaagaggcg cagtcattaa cagtgcggtg aggaattggc
      901 tggaaacgga gccatcaacg tcaacgttgc cgccagtaaa gccgccctgt aaacgcaacg
      961 gcggataatt ttcttgttcc aggtaagtga tcttcgcccc gcccaggcgc agcgcatcca
     1021 ccagatgacc aatcgggcgt tctttcatac gcggctcacc ggtcagcaca atatcattgc
     1081 tacccagaca aagagctgcc gccagcggac gcattgccgt tccggcgtta ccgaggaaca
     1141 actccagggc accttctgcg tgtaatggac cgccgttacc gataatttcg caacgcgtac
     1201 gatcggctga aagcgtatag cttaccccta acgctgttaa tgcattcagc atatggcgca
     1261 cgtcatcgct atccagcaga ttggttaata ctgttttgcc gtgtgctaat gccgccagca
     1321 ataaagcgcg gttagaaacg ctcttggaac cgggcagatt aatagtgcca tcgacacgag
     1381 cgatgggttg taacgtcagg gattccatga actcaactct ctacaacaga aataaaaacc
     1441 ccacaggctg gctgtgggga ttaagcaaaa tttcggcatt aaccgtgacg gcgttcgaac
     1501 tcaaccatga agtctgtcag cgctttaacg ccttccagcg gcatggcgtt ataaatagaa
     1561 gcgcgcattc cgccgaccac acggtgacct ttcagtgcat gaaggccagc agcaaaagac
     1621 tcttcaagga acaatttgtc aagcgcactg tccgccaact ggaacggcac gttcatccgc
     1681 gaacggttag ctttcgccac gtcattgcgg tagaaatcgc tgttatcaat caccccatat
     1741 agcagttctg ctttttgctg attgatttta tccatttcag ctacaccgcc gttcgctttc
     1801 agccatttaa agaccagacc agatagatac caggcaaatg tcggcggcgt gttaaacatg
     1861 gagccgttat cgttgaggat ggaataatcc agaatcgacg gacacgcgat attcgctttg
     1921 cccagcaaat cttcacgaac gatgacgatt gtcaggccag ccgggccgat atttttctgc
     1981 gcgccagcgt aaatcacgcc ataacggctg acgtcaatcg gacgggaaag aatggttgaa
     2041 gagaagtcgg cggcgaccac cacatctttg ccgaagtctg gcgtttcgtc gatggcgata
     2101 ccgtcgatgg tttcattcgg gcagtaatgc atataagcag cattatcaga gagttgccat
     2161 tcacgcattg gcttaaccgc gcgcagacca tcaacagtca ctttggcgtc aaagacatta
     2221 ggcgtgcagt attttttcgc ttctttaatg gcacttgccg cccagtaacc ggcatcaaca
     2281 taatctgcgg tggttttatc accgagaata ttcagcggta ccgcagcaaa ctgaccgcga
     2341 ccaccgccat ggcagaataa taccttgtag ttggagggga cattaagaag atcgcgaaaa
     2401 tccttctcgg cttcctctgc aacctgaatg aactctttgc cacggtgact cacttccatc
     2461 accgacgtac caagaccgtt ccagtcgcgc agttcctgtt gagcctgttt aagcacctct
     2521 gccggtagca ttgccggacc agaactaaaa ttgaagattt gagccatttc ccctcaccac
     2581 gttgcgttgc tattaacccg cggtcaatcg attgcgacca caggtaatac attattcctg
     2641 tggctatcgg ttttatcatt cagtgacaca catcgcaatg agtaaaaccg ttgagggcaa
     2701 aaatgtggct tgcatcacac aaaagaatct ctcggattat tagcaaaaag ccgatgtttt
     2761 tactgtcagg tcacccgttt tgcccgactt gccttcgtcc attctggatt tgcacagcgc
     2821 ggacataatt gtttttcccc cgctttcata tgaatgattt cactacaacg aatacaggca
     2881 tagtccgccg actccggcac cactttgtaa cgctgcgtac aatcactggg taaaataagt
     2941 aaacccggtt tcaccttgtc atcttcaaag aaatagacac tgatttgccc gttggtttcg
     3001 agaatcgcca gacgtacctg ccccagctgc tccacgccac gcaatcgtag ctccataaag
     3061 aactcaaatt ccgtcatgtt ggagttattg agtttcgacc aggccagctc gccatcttca
     3121 ataataacaa ccggcttacc ttccagtaga tcttccagtt tttcactgtg cgccatcaac
     3181 cacattacca ggcggtataa caacgccaga gtgataaaga cgataagtac cggcaccatc
     3241 ggcacatcat cataaaacgc cacatctccc gccgctgaac ccagcgtgag aatgattaac
     3301 acttcaaaca gcgacatctg tcgcacaccg cgtcttccgg tcattttgag gaacaaaaaa
     3361 accagtacaa aggtataaag actacgtagt gcaacttcgc caaggaaatc aaaaggcact
     3421 ttatcaaatg ccatacggtg gagatcgaac gccttcattt ttatgctctt atcgtggttt
     3481 atccgctaaa agcatagtca acagttcatc ggcatggcgg cggtgatagc gcctttgaga
     3541 taaaacccgt attattgcgc gctttccgta cgactaaagt gattttcatg acgcaaacat
     3601 ttatccccgg caaagatgcc gctctggaag attccatcgc tcgcttccag caaaaacttt
     3661 cagacctcgg ctttcagatt gaagaggcct cctggctgaa tcctgtgcct aacgtctggt
     3721 ctgtacatat tcgcgacaaa gagtgcgcac tgtgttttac caacggaaaa ggcgcaacca
     3781 aaaaagcggc gctggcttct gcacttggtg aatatttcga gcgtctctca accaactact
     3841 tttttgctga tttctggctg ggcgaaacca tcgccaacgg tccgttcgtg cattatccca
     3901 acgaaaaatg gttcccactg accgaaaatg acgatgtgcc agaaggacta ctcgatgacc
     3961 gtctgcgcgc gttttatgat ccggagaatg aactgaccgg cagcatgctg attgacctac
     4021 aatccggtaa cgaagatcgt ggtatttgcg gcctgccgtt tacgcgtcag tccgacaatc
     4081 agaccgttta tattccgatg aatatcattg gtaacctgta cgtctccaac ggtatgtccg
     4141 caggtaatac ccgcaacgaa gcacgcgttc agggattgtc tgaagttttc gaacgctacg
     4201 tgaaaaaccg cattattgct gaaagcatca gcctgccaga gatcccggca gacgtgctgg
     4261 cgcgttaccc agcagtagtt gaagccatcg aaacgctgga agcggagggt ttcccaatct
     4321 tcgcttatga cggttcgctt ggcggccagt atccggtgat ttgcgtggta ctgttcaatc
     4381 ctgctaacgg tacctgcttt gcctctttcg gtgcgcatcc tgattttggc gtagcactgg
     4441 aacgtaccgt gaccgagctg ctgcaaggtc gtggcctgaa agatttggat gtgtttactc
     4501 cgccaacctt cgatgatgaa gaagtcgctg aacataccaa cctcgaaacg cactttatcg
     4561 attccagcgg tttaatctcc tgggacctgt tcaagcagga tgccgattat ccgtttgtgg
     4621 actggaattt ctccggcacc acggaagaag agttcgccac gctgatggct atcttcaaaa
     4681 aggaagataa agaagtttat attgccgatt acgagcatct gggtgtctac gcttgccgca
     4741 ttatcgtgcc tggcatgtcc gatatttatc cggctgaaga tctgtggctc gcgaataaca
     4801 gtatgggcag ccatttacgt gaaacgattc tttcgctacc aggcagcgag tgggaaaaag
     4861 aagattacct gaacctcatc gagcaactgg atgaagaagg ttttgatgac tttacccgcg
     4921 tgcgtgagct gttgggtctg gcgaccgggt cggataacgg ttggtacacc ctgcgtatcg
     4981 gtgaattaaa agccatgctg gcgctggctg gtggcgatct ggaacaggct ctggtctgga
     5041 ccgaatggac gatggagttt aactcatcag tatttagtcc ggaacgcgcc aactattatc
     5101 gctgcctgca aacgttgtta ttactggcac aggaagaaga tcgccagccg ctgcaatatc
     5161 tgaatgcgtt tgttcgcatg tacggcgcag atgccgtaga agccgccagt gcggcaatga
     5221 gcggcgaagc ggcgttttac ggcctgcaac cggtagatag cgatctgcac gcgtttgctg
     5281 cacatcagtc gttgttgaag gcctacgaaa agctgcagcg cgccaaagca gcattctggg
     5341 caaaataaaa tcaaatagcc tacgcaatgt aggcttaatg attagtctga gttatattac
     5401 ggggcgtttt tttaatgccc cgctttacat atatttgcat taataaaata attgtaatta
//
LOCUS       DAPHDW010000210         5437 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.213, whole genome shotgun sequence.
ACCESSION   DAPHDW010000210 DAPHDW010000000
VERSION     DAPHDW010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5437
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.213"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(102..343)
                     /locus_tag="RH057_004240"
                     /pseudo
     CDS             complement(102..343)
                     /locus_tag="RH057_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            522..1172
                     /gene="inaA"
                     /locus_tag="RH057_004241"
     CDS             522..1172
                     /gene="inaA"
                     /locus_tag="RH057_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HDV3130517.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYAQHAVSPYSDEVRQAMLKAVALAFKKMHSINRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            complement(1226..1480)
                     /gene="yfaE"
                     /locus_tag="RH057_004242"
     CDS             complement(1226..1480)
                     /gene="yfaE"
                     /locus_tag="RH057_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HDV3130518.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEM"
     gene            complement(1480..2610)
                     /gene="nrdB"
                     /locus_tag="RH057_004243"
     CDS             complement(1480..2610)
                     /gene="nrdB"
                     /locus_tag="RH057_004243"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HDV3130519.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            complement(2699..4984)
                     /gene="nrdA"
                     /locus_tag="RH057_004244"
     CDS             complement(2699..4984)
                     /gene="nrdA"
                     /locus_tag="RH057_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HDV3130520.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPAIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
BASE COUNT         1319 a         1303 c         1432 g         1383 t
ORIGIN      
        1 gtaaatgggc tgtttactga tttccctgat aaggcagtaa aattccttaa taaagagtaa
       61 tgctactgca ggatggcgtt catgcgccat cctgtctcat atcaaatttt tccattcacc
      121 acgagttgtt ccgtacacgt ttgtagtaaa aacagtggac tgccttctac cggcttttct
      181 ttagccaaca acgaaatctg tcgatagaag gttggttcgt gaggaacgga cacaattcac
      241 ctgcaatgct tttcagcgtt aactctggtt gtaaggcgat gcctaatccc tgtcgaataa
      301 aattcatcgc tgtatctgac tgattaaatt cacattttat ctgctgggcg cactattagt
      361 ggtagtggct ggtggaatct atgcgctgca aatgaagggc aaaagcggtg tcacacctgt
      421 agcggcaaaa gaaattcatt aatacgacac gtttcattaa gattttcctc aggtaaccac
      481 ctatagtcat tgtcgttaat aaaaatgaca ggcgataggg tatggcagtt tcagcaaagt
      541 acgacgaatt taatcactgg tgggcaacag agggcgactg ggttgaagag cctaactatc
      601 gccgtaatgg tatgagcggg gtgcaatgcg tcgagcgcaa cggcaaaaag ctgtatgtaa
      661 agcgcatgac gcatcatctg tttcattctg tacgttatcc gttcggccga ccaacgattg
      721 tccgtgaggt tgcggtaatt aaagaactgg aacgggccgg tgtcatcgtg ccgaagatcg
      781 tttttggtga agcggtgaaa attgagggtg aatggcgagc gttgctggtg actgaagata
      841 tggcggggtt catcagcatt gctgactggt atgcccagca tgcagtatcg ccttattctg
      901 acgaagtacg gcaagccatg ttgaaagcag tggcgctggc gtttaagaaa atgcatagca
      961 ttaatcgtca gcatggctgt tgttatgttc gccatatcta tgtgaaaaca gaaggtaagg
     1021 cagaagctgg ttttcttgac ctggagaaaa gtcgtcgccg cttgcgtcgc gataaagcga
     1081 ttaatcatga cttccgccag ctggaaaaat atctggagcc aatccccaaa gcggactggg
     1141 agcaggtgaa agcgtattac tacgccatgt aatcgttgta ggccagataa gacgcataag
     1201 cgttgtatct ggcactacaa ccaattcaca tctcgatttc aatatcgcct tttgcccggc
     1261 aacaacaggg caaaatttcc cccggctgaa taaaggctaa cggttcggca atccagtcaa
     1321 cttgacctgc aaccaggcgt gtgcgacagg agccgcagta accttcgcga cactgatact
     1381 caaccgccac attgtgggat tccagcgccg ccagaaggga agggtgttca tcctggcaca
     1441 gcagttgtgt gccagtgatg cgcagggtaa cgcgggccat cagagttgga agttactcaa
     1501 atcgtcggtg tccacttccg cgtcaatctg cccgaccaga taagaactga cttccacttc
     1561 ctgcggagca acctgcacgt tatcagacac cagccaggtg ttgatccatg ggatcgggtt
     1621 ggagcgcgtc tggaacggca gatccaaacc gactgcctgc atacggatat tggtgatgta
     1681 ttcaacgtac tggcagagaa tatctttatt cagaccaatc atcgaaccgt cacggaacag
     1741 ataatccgcc cagtctttct cctgttgagc tgcctgaaca aacaggtcat agcactcctg
     1801 cttacactct tcggcaattt ccgccatctc aggatcgtcc gcgccgctgc gcagcagatt
     1861 cagcatatgc tgggtgccgg tcaggtgcag ggcttcgtcg cgggcaatca ggcgaataat
     1921 tttggcgttg ccttccatca attcgcgttc tgcaaaggca aaggaacaag caaagctgac
     1981 gtagaagcgg atcgcttcca gcgcgttaac gctcatcagg caaagataca gttttttctt
     2041 cagctcgcgc aggctaacgg ttacggtttt accgttaacg gtgtgggtgc cttcgcccag
     2101 aaggtgccag tagctggtca tttcgatcag ttcatcgtaa tagctggaga tcccttccgc
     2161 acgtttctgg atctgctcgt tggtgacgat atcgtcaaac acaacagacg gatcgttaac
     2221 gatattacga atgatatgag tataggaacg ggaatgaatc gtttctgaga acgcccaggt
     2281 ttcgacccag gtttccagtt ccggaataga aataagcggc aatagcgcca cgttcggact
     2341 acgaccctga atggagtcca gcagcgtctg atatttcagg ttgctgataa agatgtgttt
     2401 ttcgtgctcc ggcagcgcct ggtaatcgat acggtcgcgg gagacgtcaa cttcttccgg
     2461 acgccagaag aaagagagct gcttttcgat cagcttttcg aagatgtcat atttttgctg
     2521 atcgtagcga gccacgttga ccggctgacc aaaaaacatc ggttctttaa gctgatcatt
     2581 tttcgtctgt gaaaaggtgg tatatgccat gagtgtgtcc tgtggtttta ttacccctca
     2641 ccctaaccct ctcccgcaag gggagagggg acggtttggt gcaaaggctg ggggattatc
     2701 agatcttaca tgcgccgctt tcgcagccat cgtcctggat tgacggcacc agatcgtcct
     2761 gcgcgtcttc agcgccgtca cgggtgttct gataatacag cgttttgacc ccgaatttgt
     2821 aggcggtgag caggtctttc agcaactgct gcatcggcac ttttcctgac gggaaacgtg
     2881 acggatcgta gttggtgtta gcagagatcg actgatcgat aaatttctgc atgataccta
     2941 ccagttgcag gtagccatcg ttacccggca tttcccacag cagctcatag gcgtcgtgca
     3001 ggtgctcgta gtccggcacc acctggcgca aaataccgtc tttcgacgct ttgatgctga
     3061 cgtaaccgcg cggcggttca ataccgttag tggcgttaga gatctgcgaa gaggtctcgg
     3121 acggcatcag ggcagaaagc gtggagttac gcaggccgtg cgttttgatt gattcacgca
     3181 atgcttccca gtcgtaatgc agcggctcat tagcgatggc atccagatct ttcttatagg
     3241 tatcgatcgg caggatccct ttcgcgtaag tggtttcgtt aaaccacggg cacgcgcctt
     3301 gctctttagc cagctcatta gacgctttca gcaggtaata ctgaatcgct tcgaaggttt
     3361 tatgcgtcag gttgttggcg ctgccgtcgg agtagcgttt accgtgcttc gccagatagt
     3421 aagcgaagtt aatcacacca atacccagcg tacgacgccc catcgctcca cgtttggcgg
     3481 ccgggatcgg gtaatcctga taatccagca gcgcgtcaag tgcacgaacc gccagaattg
     3541 ccagctcttc cagttcatcc aggctattaa ttgcgcccag gttgaaagca gacagcgtac
     3601 acagcgcgat ttcaccgttc tcgtcgttga cgtcgttcag cggtttggtc ggcagggcta
     3661 tctccaggca caggttagac tgacgcactg gcgcgatggc cggatcaaac gggctatggg
     3721 tattgcagtg gtcaacgttc tgaatataga tacgaccggt agacgcacgt tcctgcatca
     3781 tcagcgagaa cagctcaacg gctttcacac gctgcttgcg gatgctgtcg tctttctcat
     3841 atttggtata caggcgttca aactcttcct gatcggcgaa gaatgcgtcg tacagccccg
     3901 gtacgtcgga cgggctgaac agggtgatat cttcaccttt cagcaggcgg gtatacatca
     3961 gtttgttgat ctgtaccccg tagtccatat ggcgcacgcg gttgccttcc acaccacggt
     4021 tgtttttcaa caccagcagg ctttccactt ccagatgcca catcgggtag aacagcgttg
     4081 ccgcaccgcc gcgcacgccg ccctgagagc aggatttcac cgctgtctgg aaatgtttgt
     4141 agaacggaat acagccggta tggaacgctt caccaccgcg aatcgggcta cccagcgcac
     4201 gaatacgccc ggcgttgatg ccgatcccgg cacgctggga aacgtatttc acaatcgcgc
     4261 tggaggtagc gttgatggaa tccagactgt caccgcactc gatcagtacg caggagctga
     4321 actgacgagt cggggtacgc acgccggaca tgattggcgt cggcagcgaa attttaaatg
     4381 tggaaaccgc gtcgtaaaaa cgcttcacat attgcaggcg cgtttcacgc gggtagttcg
     4441 agaacaggca cgcggcaacc agaatataaa ggaactgggc gctttcgtag atttcgccgg
     4501 tcacgcggtt ctgtaccaga tatttgcctt ccagctgctt aacggcagca taagagaagg
     4561 tcatatcacg gtcgtgatcg ataaacgtgt ccatctgctt gaactcttct tccgtgtagt
     4621 cttccagcag atgattatcg tatttgccca tctcgaccat tttcaccacg tggtcgtaca
     4681 gcgcaggcgg ctcaaactgg ccgtaggctt ttttacgcag gtggaagatc gccaggcgcg
     4741 cggcgagata ctgataatcc ggcgcatcac gggagatcag gtctgcggcc gccttgataa
     4801 tggtttcgtg gatgtcagag gtcttgatac cgtcataaaa ctgaatgtgg gagcgcagct
     4861 cgacctggga aatcgaaacg ttatgcagtc cttctgccgc ccaatccaga acgcgatgga
     4921 ttttgtcgag attgatgcgc tctgtgctac cgtcgcgctt tgtcaccagc agattctgat
     4981 tcatgtatgt cgtacctgtt tttggaattc tttccgcaat agtttcatgc agactacctg
     5041 tagtacggga agctgcttag aacactatat atagggggta tgtttgggat tcactgcaag
     5101 atagtgtgaa aatggccctc ttgcaagtgc ataactttgt ggataactca ggaaggaaaa
     5161 agtggctttc gcgcacctta ggtcagacaa ggtgtccggg aaagtcaatg ggaagagaaa
     5221 aatttgttaa aaataactgt tcggcgattt cttgaacggc agaataggtt ggataaattg
     5281 atatagatgg tttattaatc tttttgctaa tgattgcaaa aattgatcta agcacgagtc
     5341 atcacataat ctgatgtatc agttgtggtc aatatataag cgaatgtaag cgattgttga
     5401 tgtcgaaatg gttgttttct attcggttat tgttaaa
//
LOCUS       DAPHDW010000211         5314 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.214, whole genome shotgun sequence.
ACCESSION   DAPHDW010000211 DAPHDW010000000
VERSION     DAPHDW010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5314
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.214"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            162..860
                     /locus_tag="RH057_004245"
     CDS             162..860
                     /locus_tag="RH057_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000577178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HDV3130521.1"
                     /translation="MIFQKIARLLKSEINGNSWHVGDLLPSEAELAVRYNVSRNTLRK
                     ALSLLEGEGIIHRKHGSGTYIQKKNFVAHIDHMNSFSEIAHKSGKEAGSQIMKFEVQD
                     ASPTIATELNLVTGEQVYYIKRLRFIEDNAAQLEETWMSVARFPDLTVSHMQKSKFSY
                     IENECGIKIIGTFETFSPTFPTPEIASILRISPRDPILKIQTQAVDSNSIPLDYSLLY
                     SNIFEFQVKYFFPR"
     gene            complement(905..1804)
                     /gene="eamA"
                     /locus_tag="RH057_004246"
     CDS             complement(905..1804)
                     /gene="eamA"
                     /locus_tag="RH057_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HDV3130522.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVASLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVRG"
     gene            1999..3186
                     /gene="ydeE"
                     /locus_tag="RH057_004247"
     CDS             1999..3186
                     /gene="ydeE"
                     /locus_tag="RH057_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HDV3130523.1"
                     /translation="MNLSLRRSTSALLASSLLLTIGRGATLPFMTIYLSRQYSLSVDL
                     IGYAMTIALTIGVVFSLGFGILADKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLF
                     FALINCAYSVFATVLKAWFADNLSSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSI
                     NLPFWLAAICSAFPMLFIQIWVKRSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLA
                     SFVSGAFASCISQYVMVIADGDFAEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRAL
                     MTAGTLCFVIGLVGFIFSGNSLLLWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKAS
                     YFSAQSLGWLGAAINPLVSGVVLTSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQP
                     ALC"
     gene            3313..3408
                     /gene="mgtS"
                     /locus_tag="RH057_004248"
     CDS             3313..3408
                     /gene="mgtS"
                     /locus_tag="RH057_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HDV3130524.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHKWDD"
     gene            complement(3627..4517)
                     /gene="dgcZ"
                     /locus_tag="RH057_004249"
     CDS             complement(3627..4517)
                     /gene="dgcZ"
                     /locus_tag="RH057_004249"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcZ"
                     /protein_id="HDV3130525.1"
                     /translation="MIKKTTEIDAILLNLNKAIDAHYQWLVSMFHSVVARDASKPEIT
                     DNHSYGLCQFGRWIDHLGPLDNDELPYVRLMDSAHQHMHNCGRELMLAIVENHWQDAH
                     FDAFQEGLLSFTAALTDYKIYLLTIRSNMDVLTGLPGRRVLDESFDHQLRNTEPLNLY
                     LMLLDIDRFKLVNDTYGHLIGDVVLRTLATYLASWTRDYETVYRYGGEEFIIIVKAAN
                     DEEACRAGVRICQLVDNHAITHSEGHINITVTAGVSRAFPEEPLDVVIGRADRAMYEG
                     KQTGRNRCMFIDEQNVINRV"
     gene            complement(4772..5164)
                     /gene="ydeI"
                     /locus_tag="RH057_004250"
     CDS             complement(4772..5164)
                     /gene="ydeI"
                     /locus_tag="RH057_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HDV3130526.1"
                     /translation="MKFQAIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHKGEDRYVFRDKSGEINAVIPAAVFDGREV
                     QPDQMINISGSLDKKSAPAVVRVTHLQK"
BASE COUNT         1469 a         1269 c         1119 g         1457 t
ORIGIN      
        1 aaatatcata tcccgcagta gctatacgtc ctggtacatt gatcacagta atacagtgat
       61 aaagttgttc aacatcaggg tgattattaa acgactcggc catacgataa aacaatcagg
      121 actcaactcc ttgttttcaa tggctcttaa aaggtgcaaa agtgattttt caaaagattg
      181 cccgcttact taaatccgaa atcaatggca attcatggca tgttggtgat ttgttgccgt
      241 cagaagcgga actcgctgtt cgttataatg tttcgcgtaa taccctgcgt aaggcattat
      301 ccttgctgga aggcgaagga attattcaca gaaagcatgg ttcaggaaca tacattcaaa
      361 aaaagaattt tgttgcacac attgatcaca tgaacagttt cagtgaaatt gcgcataaaa
      421 gcggcaaaga ggcagggagt cagattatga aatttgaagt gcaggatgct tcacccacta
      481 ttgcgactga gctgaattta gtgactggtg agcaggttta ttacataaag agactgcgat
      541 tcattgagga taatgcagca caattagaag aaacgtggat gtcagtagca cgttttcctg
      601 atttaaccgt gtcacatatg caaaaatcca aattttcata tattgagaat gaatgcggga
      661 tcaaaatcat tggcaccttt gaaactttct cccctacttt tcctacccca gaaatcgcca
      721 gtattttacg gatcagccca cgggatccca tacttaaaat tcagacccag gctgtggaca
      781 gtaactctat tccgctggat tattcgttac tttacagcaa tattttcgag ttccaggtaa
      841 agtacttttt cccgcgataa aagcagacgc acagagtgct gacatcacat tctgtgcgta
      901 tggtttagcc tctaaccttt accgctttac gccaccgcaa gccaaataca ttgatataca
      961 gcccggtcat aatgagcacc gcacctaaaa attgcagacc cgttaagcgt tcatccaaca
     1021 atagtgccgc acttgccagt ccgactacgg gcaccagtaa cgataacggt gcaacccgcc
     1081 aggtttcata gcgtcccagt aacgtccccc agatcccata accaacaatt gtcgccacaa
     1141 acgccagata catcagcgac aagatggtgg tcatatcaat agtaaccaga ctgtgaatca
     1201 tggatgcgga accatcgaga atcagcgagg caacaaagaa gggaatgatt gggattaaag
     1261 cgctccagat taccagcgac atcaccgccg gacgcgttga gtgcgacatg atctttttat
     1321 tgaagatgtt gccacacgcc caactaaatg ctgcagccag ggtcaacata aagccgagca
     1381 tcgccacatg ctgaccgttc agactatctt cgattaacac cagtacgcca aaaatcgcta
     1441 aggcgatccc cgccaattgt ttgccatgca atcgctcccc gaaagtaaac gcgccaagca
     1501 cgatagtaaa aaacgcctgt gcctgtaaca ccagcgaagc cagtccagca ggcataccga
     1561 agttaatggc acaaaaaaga aaagcaaact gcgcaaaact gatggttaat ccatacccca
     1621 gcagcaaatt cagtggtact ttcggtcgtg cgacaaaaaa gatagccgga aaagcgacca
     1681 gcataaagcg caaaccggcc agcatcagcg gtggcatgtt atgaagcccc actttgatga
     1741 ccacaaaatt tagcccccat acgaccacta ccagtagcgc caacacccca tcttttcgcg
     1801 acattctacc gcctctgaat ttcatctttt gtaagcaatc aacttagctg aatttacttt
     1861 tctttaacag ttgattcgtt agtcgccggt tacgacggca ttaatgcgca aataagtcgc
     1921 tatacttcgg atttttgcca tgctatttct ttacatctct aaaacaaaac ataacgaaac
     1981 gcactgccgg acagacaaat gaacttatcc ctacgacgct ctaccagcgc ccttcttgcc
     2041 tcgtcgttgt tattaaccat cggacgcggc gctacgctgc catttatgac catttacttg
     2101 agtcgccagt acagcctgag tgtcgatcta atcggttatg cgatgacaat tgcgctcact
     2161 attggcgtcg tttttagcct cggttttggt atcctggcgg ataagttcga caagaaacgc
     2221 tatatgttac tggcaattac cgccttcgcc agcggtttta ttgccattcc tttagtgaat
     2281 aacgtaacgc tggtggtgct cttttttgcc ctcattaact gcgcctattc tgtttttgct
     2341 accgtgctga aagcctggtt tgccgacaat ctttcgtcca ccagcaaaac gaaaatcttc
     2401 tcaatcaact acaccatgct aaacattggc tggaccatcg gtccgccgct cggcacgctg
     2461 ttggtgatgc agagcatcaa tctgcccttc tggctggcag ctatctgttc cgcgtttccc
     2521 atgcttttca ttcaaatttg ggtaaagcgc agcgagaaaa tcatcgccac ggaaacaggc
     2581 agtgtctggt cgccgaaagt tttattacaa gataaagcac tgttgtggtt tacctgctct
     2641 ggttttctgg cttcttttgt aagcggcgca tttgcttcat gcatttcaca atatgtgatg
     2701 gtgattgctg atggggattt tgccgaaaag gtggtcgcgg ttgttcttcc ggtgaatgct
     2761 gccatggtgg ttacgttgca atattccgtg ggccgccgac ttaacccggc taacatccgc
     2821 gcgctgatga cagcaggcac cctatgtttc gtcatcggtc tggtcggttt tattttttcc
     2881 ggcaacagcc tgctattgtg gggtatgtca gctgcggtat ttactgtcgg tgaaatcatt
     2941 tatgcgccgg gcgagtatat gttgattgac catattgcgc cgccaggaat gaaagccagc
     3001 tatttttccg cccagtcttt aggctggctt ggtgccgcga ttaacccatt agtgagtggc
     3061 gtagtgctaa ccagcctgcc gccttcctcg ctgtttgtca tcttagcgtt ggtgatcatt
     3121 gctgcgtggg tgctgatgtt aaaagggatt cgtgcaagac cgtgggggca gcccgcgctt
     3181 tgttgattta agtcgaacac aataaaaatt taattcagcc ttcgtttagg ttacctctgc
     3241 taatatcttt ctcattgaga tgaaaattaa ggtaagcgag gaaacacacc acaccataaa
     3301 cggaggcaaa taatgctggg taatatgaat gtttttatgg ccgtactggg aataatttta
     3361 ttttctggtt ttctggccgc gtatttcagc cacaaatggg atgactaatg aacggagata
     3421 atccctcacc taaccggccc cttgttacag ttgtgtacaa ggggcctgat ttttatgacg
     3481 gcgaaaaaaa accgccagta aaccggcggt gaatgcttgc atggatagat ttgtgttttg
     3541 cttttacgct aacaggcatt ttcctgcact gataacggat cgttgacaca gtagcatcag
     3601 ttttctcaat gaatgttaaa cggagcttaa actcggttaa tcacattttg ttcgtcaata
     3661 aacatgcagc gatttcttcc ggtttgctta ccctcataca ttgcccggtc tgctcttcca
     3721 atgaccacat ccagaggctc ttcaggaaat gcgcgactca cacctgctgt cacggtaatg
     3781 ttgatatgcc cttcagaatg tgtgatggca tggttatcga ctaactggca aattctgaca
     3841 cctgcacgac atgcttcttc atcattagcc gctttgacaa taatgataaa ttcttcgccc
     3901 ccgtagcgat aaaccgtttc gtaatcacgc gtccaactgg ctaagtaagt tgccagggtg
     3961 cgtaatacta catcgccgat taaatgcccg taggtatcat taaccaattt aaatcggtca
     4021 atatccaaca acattaaata aagattcaga ggctcagtgt tgcgtaactg atgatcaaag
     4081 gattcatcaa gaacccgacg ccccggcaat cccgtcaaaa catccatatt gctacggatc
     4141 gtcagcaaat aaattttgta atcggttaat gccgcagtaa aagaaagcaa cccctcctga
     4201 aaggcgtcga aatgcgcgtc ctgccagtga ttttcaacaa tagccagcat taattcccga
     4261 ccacagttat gcatatgttg atgggcagaa tccattagcc gaacgtaagg taattcatcg
     4321 ttatcgagtg gccccagatg atcaatccac cgaccaaact ggcacagtcc ataagaatgg
     4381 ttatccgtta tttctggctt actggcatct ctcgcgacca cgctgtgaaa catactcacc
     4441 agccactggt agtgggcatc gatagcctta ttgagattta acaagatggc atcaatttcc
     4501 gttgtcttct tgatcattgc cactcctttt tcacagttcc ttgtgcgcgc tattctaacg
     4561 agagaaaagc aaaattacgt caatattttc atagaaatcc gaagttagga gtcatctctg
     4621 agataacatt gtgatttaaa acaaaatcag cggataaaaa agtgtttaat tctgtaaatc
     4681 acctctgcat tatcgtaaat aaaaggatga caaatagcat aacccaatac cctaatggcc
     4741 cagtagttca ggccatcagg ctaatttatt tttatttctg caaatgagtg acccgaacga
     4801 cggccggcgc gcttttctta tccagactgc cgctaatgtt gatcatctgg tccggctgaa
     4861 cttctcgtcc atcaaagacg gccgcaggaa taacggcatt aatttcaccg ctcttatcgc
     4921 gaaaaacgta acggtcctct cctttgtgag aaatcaaatt accgcgtagt gaaaccgaag
     4981 cgccatcgtg catgtttttt gcgaaatcaa cggtcatttt ttttgcatca tcggttccgc
     5041 gatagccatc ttctattgca tgaggcggcg gtggcgctgc atcctgtttt aaaccgccct
     5101 ggtcatctgc caacgcataa ggcatgacaa gaaaacttgc taatacaatg gcctgaaatt
     5161 tcatactaac tccttaattg cgtttggttt gacttattaa gtctggttgc tatttttata
     5221 attgccaaat aagaatactg ccaattgtta taaggcattt aaaatcagcc cactagctgt
     5281 caaatattca gagaatttaa ctcactaaag ttaa
//
LOCUS       DAPHDW010000212         5290 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.215, whole genome shotgun sequence.
ACCESSION   DAPHDW010000212 DAPHDW010000000
VERSION     DAPHDW010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5290
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.215"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..68)
                     /locus_tag="RH057_004251"
     CDS             complement(<1..68)
                     /locus_tag="RH057_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130527.1"
                     /translation="MKSSTTIITAYFDIGRGDWTAN"
     gene            372..1304
                     /gene="rfaD"
                     /locus_tag="RH057_004252"
     CDS             372..1304
                     /gene="rfaD"
                     /locus_tag="RH057_004252"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HDV3130528.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LNIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            1314..2360
                     /gene="rfaF"
                     /locus_tag="RH057_004253"
     CDS             1314..2360
                     /gene="rfaF"
                     /locus_tag="RH057_004253"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HDV3130529.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYIALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            2364..3356
                     /gene="rfaC"
                     /locus_tag="RH057_004254"
     CDS             2364..3356
                     /gene="rfaC"
                     /locus_tag="RH057_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HDV3130530.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            3353..4561
                     /gene="rfaL"
                     /locus_tag="RH057_004255"
     CDS             3353..4561
                     /gene="rfaL"
                     /locus_tag="RH057_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HDV3130531.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEIFKAII
                     ENTLLCTIAIPIILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVAIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(4597..>5290)
                     /locus_tag="RH057_004256"
     CDS             complement(4597..>5290)
                     /locus_tag="RH057_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HDV3130532.1"
                     /translation="KKYYQSYLANADIEIVPNGIDLETYQSNFQPITRSELNISPEEK
                     IIFYAGRIVPDKGILLLMQSFEKLAAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIA
                     KRLKDRCIMLGSIPPEKMHCYYPLADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRG
                     GMTEFVKENTTGFHLKEPMTADSISSDILKTLANPELTAVAKQGQDFVFEHYSWDGVT
                     QRFEEVIHNWFE"
BASE COUNT         1449 a         1173 c         1206 g         1462 t
ORIGIN      
        1 ttattagcag tccaatcccc tctaccaatg tcaaaatatg cagtgataat tgtggtggat
       61 gatttcatta ctaataagac cattaatata aaaaaagaat tatatcataa atattaacag
      121 aatgtatact gtatattatc cattcaaatg cttccgttat ttatcataca tcggaatatt
      181 gatactaaag cactattcac atgcaaaacc aacatccgcc atgaaggact agctaaaacc
      241 caaactagtt tgttgcaatt agcatccttg cacctctatg taaagggctg aagggattcg
      301 gatgtgatgg tatgattaca gacattcgtg tctgagattg tctctgactc cataattcga
      361 aggttacagt tatgatcatc gttaccggcg gcgcgggctt tatcggcagc aacatcgtta
      421 aagccctgaa tgataaaggc atcaccgata ttctggtggt ggacaacctg aaagacggca
      481 ccaagtttgt gaacctggtg gatctgaata tcgcagacta tatggataag gaagacttcc
      541 tgatccagat tatggctggc gaagagttcg gcgatgtcga agcgattttc cacgaaggcg
      601 cgtgctcttc caccaccgag tgggacggca agtatatgat ggataacaac tatcaatact
      661 ccaaggagct gctgcactac tgcctggagc gtgaaatccc gttcctgtac gcttcttccg
      721 cagccaccta cggcggacgc acctccgact ttattgaatc ccgcgagtac gaaaaaccgt
      781 tgaacgtcta cggttactca aaattcctgt ttgatgaata tgttcgccaa atcctgccgg
      841 aagcgaactc gcagattgtt ggcttccgct atttcaacgt ttatggaccg cgtgaaggcc
      901 ataaaggcag catggcgagc gtcgctttcc atctcaacac ccagcttaat aacggtgaat
      961 caccgaagct gttcgaaggt agcgagaact ttaaacgcga cttcgtctat gttggcgatg
     1021 tggcagatgt aaacctgtgg ttcctggaaa atggcgtttc cggcatcttc aacctcggca
     1081 ccggtcgtgc ggaatccttc caggctgtag ctgatgctac gctggcttat cacaagaaag
     1141 gccagatcga atacattccg ttcccggata agctgaaagg ccgctaccag gcgttcactc
     1201 aggcagatct gacaaatctg cgcgcggcgg gttacgacaa accgttcaaa accgttgctg
     1261 aaggtgtaac ggaatacatg gcctggctga atcgcgacgc ataagagctc tgcatgaaaa
     1321 tactggtgat cggcccgtct tgggttggcg acatgatgat gtcgcaaagt ctctatcgca
     1381 cgctccaggc gcgctatccc caggcgataa tcgatgtgat ggcaccggca tggtgccgtc
     1441 cattattatc gcggatgccg gaagttaacg aagctatccc tatgcctctc ggtcacggag
     1501 cgctggaaat cggcgaacgc cgcaaactgg gtcatagcct gcgtgaaaag cgctacgacc
     1561 gcgcctacgt cttacctaac tccttcaaat ccgcattagt gcctttcttc gcgggtattc
     1621 ctcatcgcac cggctggcgc ggcgagatgc gctacggttt actcaacgat gtacgcgtgc
     1681 tcgataaaga agcctggccg ctaatggtgg aacgctatat agcgctggcc tatgacaaag
     1741 gcattatgcg cacagcacaa gatctgccgc agccattgtt atggccgcag ttgcaggtga
     1801 gcgaaggtga aaaatcatat acctgtaatc aattttcgct ttcatcagaa cgtccgatga
     1861 ttggtttttg cccgggtgcg gagtttggtc cggcaaaacg ctggccacac taccactatg
     1921 cggagctggc aaagcagctg attgatgaag gttatcaggt ggttctgttt ggctcggcga
     1981 aagatcatga agcaggcaat gagattcttg ccgctttaaa taccgagcag caggcatggt
     2041 gtcggaacct ggcgggggaa acacagcttg atcaagcggt tatcctgatt gcagcctgta
     2101 aagccattgt cactaacgat tctggcctga tgcacgttgc ggcggcgctc aatcgtccgc
     2161 tggttgccct gtatggtcca agtagcccgg acttcacacc gccgctatcc cataaagcgc
     2221 gcgtgatccg tctgattacc ggctatcaca aagtgcgtaa aggtgacgct gcggagggtt
     2281 atcaccagag cttgatcgac attactcccc agcgcgtact ggaagaactc aacgcgctat
     2341 tgttacaaga ggaagcctga cggatgcggg ttttgatcgt taaaacatcg tcgatgggcg
     2401 atgttctcca tacgttgccc gcactcactg atgcccagca ggcaatccca gggattaagt
     2461 ttgactgggt ggtggaagaa gggttcgcac agattccttc ctggcacgct gccgttgagc
     2521 gagttattcc tgtggcaata cgtcgctggc gtaaagcctg gttctcggcc cccataaaag
     2581 ctgaacgcaa agcgtttcgt gaagcgctac aagcagagaa ctatgacgca gttatcgacg
     2641 ctcaggggct ggtaaaaagc gcggcactgg tgacacgtct ggcgcatggc gtaaagcatg
     2701 gattggactg gcaaaccgct cgcgaacctt tagccagcct gttttacaat tgtaagcatc
     2761 atattgcaaa acagcagcac gccgtagaac gcacccgcga actgtttgcc aaaagtttgg
     2821 gctatagcaa accgcaaacc cagggcgatt atgctatcgc acagcatttt ctgacgaacc
     2881 tgcctacaga tgctggcgaa tatgccgtat ttcttcatgc gacgacccgt gatgataaac
     2941 actggccgga agaacactgg cgagaattga ttggtttact ggctgattca ggaatacgga
     3001 ttaaacttcc gtggggcgcg ccgcatgagg aagaacgggc gaaacgactg gcggaaggat
     3061 ttgcttatgt tgaagtattg ccgaagatga gtctggaagg cgttgcccgc gtgctggccg
     3121 gggctaaatt tgtagtgtcg gtggatacgg ggttaagcca tttaacggcg gcactggata
     3181 gacccaatat cacggtttat ggaccaaccg atccgggatt aattggtggg tatgggaaga
     3241 atcaagtaga gtgtcgttct acaagtatgt ctcttgcaga tttgccagct caaaccgttt
     3301 ttcaaaattt aaatcttgaa ataataacca ataagttgac atcggagata agatgacctc
     3361 aacattattt ttctctctcg agaaaaaaaa ctggatagcg tactggaaca gagctctcgt
     3421 attcttattc attaccacct attttttggg tgggattaca aggtataaac atcttattgt
     3481 tattcttatg acaataacga caatcgtcta tctctgcaaa cggccaaaac actatctctc
     3541 actatttaaa acatttcttt ttggtagtgt tgccatatta actattgctg cattgctgtc
     3601 acttcttcaa tcccctgatg caggtgctag catgaaggaa attttcaaag ctattattga
     3661 gaatacttta ctatgcacaa tagcaatacc gatcatattg agagacgaga aaagagaaga
     3721 tgtcgaaaaa atcgttttct tctcatttat tagtgcgttg ggcttacgct gtttttctga
     3781 attgattacc tattataagg actatcaaca agggataatg ccattcgcag attatagaca
     3841 ccgtagcatt tctgactcga tggtcttttt attccctgca ttgttaaatc tctggcttat
     3901 caaatcagca aaataccgca tttcttttgt ggttctaagc gttattttta tttttctgat
     3961 attaggaact ttatccagag gggcctggct ttccgtgtta gtcattggat taatatggat
     4021 tctgatgttt aaacaatgga agttactatt agtaggagta atggttgcca tcattgcatt
     4081 gtcggttatt ttcacacata aggagatgac cgcaaagcta acgtataaac ttcaacaaac
     4141 taatagttct tatcgctatg caaatggtac tcaaggcagc gcactcgatc taatattaga
     4201 aaatcctgtt attggttatg gttacggtaa cgttgcatat aaagatgtct ataataaacg
     4261 tgtcattgat tatccagaat ggacctttag acaatcaata gggccacata attttgcgct
     4321 attcatctgg tttggcactg gtttattagg gctggtaagt cttatgatgc tatactgtgc
     4381 aatattgaaa gagtgtataa aaaatggcgt caagaataaa tatcgctcac catataatgc
     4441 atattatata atcttactat cttttatagg ttattttgtt atccgtggaa acgtagaaca
     4501 aattgaacca aatttattag gcgtttacgc cggcttatta ttagcgatga aaaacaagta
     4561 aaaaataaaa aaggctgcat aatgcggcct ttttatttat tcaaaccaat tatgaataac
     4621 ctcttcgaat ctttgagtta cgccatccca actgtagtgt tcaaacacaa agtcctgacc
     4681 ttgctttgca actgctgtta attcaggatt cgctaacgtt tttaaaatat cgctgcttat
     4741 ggagtctgca gtcataggtt cttttaaatg gaatcccgta gtattttctt tgacaaattc
     4801 cgtcatcccc ccacgcgtac tcactaaaac aggcttacca gcacccatag cttcaattgc
     4861 caccatgcaa aatggttcct ggaactggga aggaatgacg actaaatctg ccagtgggta
     4921 ataacaatgc atcttttcag gcggtatgct tcctaacata atgcaacgat ccttcaaacg
     4981 ttttgcaatt tccctgacat tgcgttgata tgcacctttg tcacttttac tcatttctgt
     5041 ataatcacca atcacaacta attttaaatt tttgtgagca gcggccaatt tttcgaatga
     5101 ttgcatcaac agcaatatgc ctttgtcagg gacgatacgc ccagcataaa aaataatttt
     5161 ctcttcaggg gagatattaa gttcgcttct agtgatcggc tgaaaattag attgataggt
     5221 ttctaaatca attccatttg gtacaatctc aatatctgca tttgctaaat aagattggta
     5281 atactttttc
//
LOCUS       DAPHDW010000213         5287 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.216, whole genome shotgun sequence.
ACCESSION   DAPHDW010000213 DAPHDW010000000
VERSION     DAPHDW010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.216"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            50..640
                     /locus_tag="RH057_004257"
     CDS             50..640
                     /locus_tag="RH057_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3130533.1"
                     /translation="MSRIFAYCRISTLDQTIENQRREIESAGFKIKPQQIIEEHISGS
                     AATSERPGFNRLLARLKCGDQLIVTKLDRLGCNAMDIRKTVEQLTETGIRVHCLALGG
                     IDLTSPTGKMMMHVISAVAEFERDLLLERTHSGIVRARGAGKRFGRPPVLNEEQKQVV
                     FERIKSGVSISAIAREFKTSRQTILRAKAKLQTPDI"
     gene            complement(738..1313)
                     /locus_tag="RH057_004258"
     CDS             complement(738..1313)
                     /locus_tag="RH057_004258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3130534.1"
                     /translation="MAFRMSEQPRTIKIYNLLAGTNEFIGEGDAYIPPHTGLPANSTD
                     IAPPDIPAGFVAVFNSDKASWHLVEDHRGKTVYDVASGDALFISELGPLPENVTWLSP
                     EGEFQKWNGTAWVKDAEAEKLFRIREAEETKNSLMQVASEHIAPLQDAVDLEIATEEE
                     TSLLEAWKKYRVLLNRVDTSTAQDIEWPALP"
     gene            complement(1313..2002)
                     /locus_tag="RH057_004259"
                     /pseudo
     CDS             complement(1313..2002)
                     /locus_tag="RH057_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415890.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber protein"
     gene            complement(2114..2359)
                     /locus_tag="RH057_004260"
                     /pseudo
     CDS             complement(2114..2359)
                     /locus_tag="RH057_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309657.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
     gene            complement(2334..2879)
                     /locus_tag="RH057_004261"
     CDS             complement(2334..2879)
                     /locus_tag="RH057_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein"
                     /protein_id="HDV3130535.1"
                     /translation="MEVNKKQLADIFGASIRTIQNWQEQGMPVLRGGGKGNEVLYDSA
                     AVIKWYAERDAEIENEKLRREVEELRQASETDLQPGTIEYERHRLTRAQADAQELKNA
                     RDSAEVVETAFCTFVLSRIAGEIASILDGIPLSVQRRFPELENRHVDFLKRDIIKAMN
                     KAAALDELIPGLLSEYIEQSG"
     gene            3268..3462
                     /locus_tag="RH057_004262"
     CDS             3268..3462
                     /locus_tag="RH057_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlcI/YnfO family protein"
                     /protein_id="HDV3130536.1"
                     /translation="MSTKNRTRRTTTRNIRFPNQMIEQINIALEQKGSGNFSAWVIEA
                     CRRRLTSEKRAYTSIQSDDE"
     gene            3822..4115
                     /gene="iss"
                     /locus_tag="RH057_004263"
     CDS             3822..4115
                     /gene="iss"
                     /locus_tag="RH057_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="increased serum survival lipoprotein Iss"
                     /protein_id="HDV3130537.1"
                     /translation="MKKMLFSAALAMLITGCAQQTFTVGNKPTAVTPKETITHHFFVS
                     GIGQEKTVDAAKICGGAENVVKTETQQTFVNGLLGFITLGIYTPLEARVYCSQ"
     gene            complement(4206..4388)
                     /gene="rzoD"
                     /locus_tag="RH057_004264"
     CDS             complement(4206..4388)
                     /gene="rzoD"
                     /locus_tag="RH057_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage lysis lipoprotein RzoD"
                     /protein_id="HDV3130538.1"
                     /translation="MRKLKMMLCVMMLPLVVVGCTSKQSVSQCVKPPPPPAWIMQPPP
                     DWQTPLNGIISPSERG"
     gene            complement(4538..4762)
                     /locus_tag="RH057_004265"
     CDS             complement(4538..4762)
                     /locus_tag="RH057_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HDV3130539.1"
                     /translation="MSPSLRKAVAAAIGGGAVAIASVLITGPSGDDGLEGVSYIPYKD
                     IVGVWTVCHGHNRDYLRSGYLHHRRPVMGC"
     gene            complement(4762..4977)
                     /gene="essD"
                     /locus_tag="RH057_004266"
     CDS             complement(4762..4977)
                     /gene="essD"
                     /locus_tag="RH057_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage lysis protein EssD"
                     /protein_id="HDV3130540.1"
                     /translation="MKSMDKLTTGVAYGTSAGSAGYWFLQLLDKVTPSQWAAIGVLGS
                     LVFGLLTYLTNLYFKIKEDKRKAARGE"
BASE COUNT         1431 a         1322 c         1178 g         1356 t
ORIGIN      
        1 ttctgagatg cattatgata tgaacaccaa tttcgtatag agtctcacta tgtctcgaat
       61 ttttgcttac tgtcggatat caacgctgga tcagaccatc gaaaatcaac gccgggaaat
      121 cgaaagtgca ggttttaaaa tcaaacctca gcaaataatc gaagaacaca ttagcggctc
      181 agcagcaacc agtgagcgtc ctggttttaa ccggttgctt gctcgcctga aatgtggtga
      241 tcaattgatt gtgacaaaac tggatcgcct tggttgtaat gcaatggata tcaggaaaac
      301 agtggaacaa ctgaccgaaa caggtatcag agtgcattgc ttagcattgg ggggcattga
      361 cctgaccagt ccaacaggaa aaatgatgat gcacgtaatt tcagcagtcg ctgaatttga
      421 acgagacctt ttacttgaac gcactcattc cgggatagta agagcccgcg gcgcagggaa
      481 acgttttggt cgaccacctg tgttaaatga agaacagaaa caggtggtat tcgaacgaat
      541 taagtcaggt gtaagtataa gtgccattgc ccgggaattc aaaacctcgc ggcaaaccat
      601 tttaagagcc aaagcaaaac ttcagacacc tgacatataa aaaataatct cggtgtgaga
      661 tgctttacgt cttccaagcc cccttccttg ccgtaaatgg aaagatacat ctaattatag
      721 aatttatatg ttttacccta cggcagtgct ggccattcaa tatcctgtgc agttgacgta
      781 tcaacacggt tcagcaacac ccgatacttt ttccaggctt ccagcaatga ggtttcttcc
      841 tccgttgcga tttccagatc tacagcatcc tgaagtggcg caatatgctc actggctacc
      901 tgcatcaggc tgttttttgt ttcttccgcc tctcggatcc ggaacagttt ttctgcttct
      961 gcatctttca cccaggctgt gccgttccac ttctgaaact ccccttccgg cgataaccag
     1021 gtaacatttt ccggtaacgg accgagttca gaaataaata acgcgtcccc tgacgctacg
     1081 tcataaaccg ttttaccccg atggtcttca acgagatgcc acgatgcctt atcactgttg
     1141 aaaacagcca caaagccagc aggaatatct ggtggtgcaa tatcggtact gtttgctggc
     1201 agacctgtat gaggcggaat atatgcgtca ccttcaccaa taaattcatt agttccggcc
     1261 agcagattat aaatttttat ggtccgtggt tgttcactca ttctgaatgc cattatgcaa
     1321 gcctcacaat atagttaaat gcgatgtttt tgacggtgtt ttccgcgtta ccagcagcgt
     1381 taacggtgat ggtgtgtcca tgtgaaccaa tcgcaacgga gtgcgtatga gcaccaatac
     1441 cgacagtatg cgcgtgtgca cctgcagatg ctgctgtgcc ggacagtgaa tggctatgat
     1501 taccatctgt actggtattc gctaaccacc ccgtagacat acctactgag ccttgtacac
     1561 cccaggtatt ttgacctgag cttgtataac catattgata agtatcttta aaaacactgg
     1621 ggttaaatcg acggccatct ctatggctgt gattaccagc tgcattcgtg ctgccactta
     1681 aactatgggt atgcgcacca gtgttattcg tggatttagt gccgtaatca aacgacgatg
     1741 tggttttcgt ccccaaatcc gtactggatg cgctggcgct gtgggtatgc gatttaatgc
     1801 cgtcctgttc ctgagacaat acggcccgac cactggcagg tttgccctta atcgtccagc
     1861 cacgcatatc agggatcacg cctgacggat aagcggctgc aagtttcggg tatgcagatt
     1921 tgtcaaaagt ctgcccctgc atcagggcat agccagacgg aacggtatct gatggccacg
     1981 ggattggtgc gccaggcgga taaaactgct ctgatggcgt atagagtgaa taaactgtac
     2041 cgtccgttaa cccttccggc ttattagcag aatatgctgg tgacgtatga atcgtcacgt
     2101 tggcattact ggtataaccg acacgggcag acttcaccac attcacctcg cggatgtagt
     2161 cgctgcccat cgcattcatg atggcccgct gaaagggcag tgtttcccag cgcccttcct
     2221 ggtatgcgga ttctttcggg agatagtaat tagcatccgc ccattcaacg gcggtctgtg
     2281 gctccggcct gaacagtgag cgaagcccgg cgcggacaaa atgccgtagc ctgttaacct
     2341 gactgttcga tatattcact cagcaacccc ggtatcagtt catccagcgc ggctgctttg
     2401 ttcatggctt tgatgatatc ccgtttcagg aaatcaacat gtcggttttc cagttccgga
     2461 aaacgccgct gcaccgacag ggggatcccg tcgagaatac tggcaatttc acctgcgatc
     2521 cgcgacagca cgaaagtaca gaatgcggtt tccaccactt cagcggagtc tctggcattt
     2581 ttcagctcct gtgcgtcggc ctgcgcacgc gtaagtcgat ggcgttcgta ctcaatagtc
     2641 cctggctgga gatctgtctc gctggcctgc cgcagttctt caacttcccg gcgcagcttt
     2701 tcgttctcaa tttcagcatc cctttcggca taccatttta tgacggcggc agaatcataa
     2761 agcacctcat tacccttgcc accgcctcgc agaacgggca ttccctgctc ctgccagttc
     2821 tgaatggtac ggatactcgc accgaaaata tcagccagct gctttttgtt gacttccatt
     2881 gttcattcca cggacaaaaa cagagaaagg aaacgacaga ggccaaaaag ctcgctttca
     2941 gcacctgtcg tttcctttct tttcagaggg tattttaaat aaaaacatta agttatgacg
     3001 aagaagaacg gaaacgcctt aaaccggaaa attttcataa atagcgaaaa cccgcgaggt
     3061 cgccgccccg taacctgtcg gatcgccgga aaggacccgc aaaatgataa taattatcat
     3121 ctacatgtca caacgtgcat ctacgccatc aaaccacgtc aaataatcaa ttatgacgca
     3181 ggtatcgtat taattgatct gcatcaactt aacgtaaaaa aaacttcaga caatacaaat
     3241 cagcgacact gaatacgggg caacctcatg tcaacgaaga acagaacccg cagaacaaca
     3301 acccgcaaca tccgctttcc taaccaaatg attgaacaaa ttaacatcgc tcttgagcaa
     3361 aaagggtccg ggaatttctc agcctgggtc attgaagcct gccgccggag actaacgtca
     3421 gaaaagagag catatacatc aatccaaagt gatgatgaat aaacatcccg gtttcttcca
     3481 ccatcgcacc ggaaaagcga ctatgagggt aaccctgcgt ctgtcagcac agtaaaaccc
     3541 ggtgtgcatc gtttttgatt attcccgcac actcacgcag aaggaattcc ccgtcgggct
     3601 acggtcatgg ttaatgcggg aatacggcga cgatacagcg cagctaaaag ggtaatggac
     3661 agaaagagcg gtttatttca ttccacagga ttctgagtgc ccccctcctc caataggctg
     3721 agcatccacc tatatagttt taattttcat caatccattt aactatcgtt taattgttgt
     3781 cacataggat tctgccgttt ttaacaatgc aggataataa gatgaaaaaa atgttgtttt
     3841 ctgccgctct ggcaatgctt attacaggat gtgctcaaca gacgtttact gttggaaaca
     3901 aaccgacagc agtaacacca aaggaaacca tcacccatca cttcttcgtt tcgggaattg
     3961 gacaggagaa aactgttgat gcagccaaaa tttgtggcgg cgcagaaaat gttgttaaaa
     4021 cagaaaccca gcaaacattc gtaaatggat tgctcggttt tattacttta ggcatttata
     4081 ctccgctgga agcgcgtgtg tattgctcac aataattgca tgagttgccc atcgatatgg
     4141 gcagctctat ctgcactgct cattaatata cttctgggtt ccttccagtt gtttttgcat
     4201 agtgatcagc ctctctctga gggtgaaata atcccgttca gcggtgtctg ccagtcgggg
     4261 ggaggctgca ttatccacgc cggaggcggt ggtggcttca cgcactgact gacagactgc
     4321 tttgatgtgc aaccgacgac gaccagcggc aacatcatca cgcagagcat cattttcagc
     4381 tttcgcatca gctaactcct tcgtgtattt tgcatcgagc gcagcaacat cacgctgacg
     4441 catctgcatg tcagtaattg ccgcgttcgc cagcttcagt tctctggcat ttttgtcgcg
     4501 ctgggctttg taggtaatgg cgttatcacg gtaatgatta acagcccatg acaggccgac
     4561 gatgatgcag ataaccagag cggagataat cgcggttatg cccgtgacat acagtccata
     4621 caccaacaat atctttatat ggtatgtagc tgacaccttc cagaccatcg tcaccactcg
     4681 gaccagtgat gagcacagac gctatggcaa cagccccacc accaatagca gctgctacag
     4741 ccttgcgtaa tgatggcgac attattcacc tctcgcagcc ttgcgcttat cttctttaat
     4801 cttgaaataa aggtttgtca ggtacgtcag caggccaaat accaggctac ccagcactcc
     4861 aattgccgcc cactgtgagg gcgtgacttt atctagcagc tgtaaaaacc agtacccggc
     4921 actacctgct gaggtgccat aggcgacacc cgttgttaac ttatccatgg atttcataac
     4981 cccacctcgc agacaaagcg ggtgtaaatt gagggaatac tacgaaacgt aacagactcg
     5041 gagtcagtga ataactcagg tattgggtta tcagctaata tcgagactca aaaaatggaa
     5101 aaacccgctc gacggcgggt ttaagctgtg tgacgaagta accactctta acagcataac
     5161 caatttttta cgtacgtaaa ccactaaatg atatttgcga gaatgctacc gagtattgaa
     5221 aacaccacta caaatacata agcaaatctc aacaaataac caacaaatta tttccagtgt
     5281 tattttt
//
LOCUS       DAPHDW010000214         5280 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.217, whole genome shotgun sequence.
ACCESSION   DAPHDW010000214 DAPHDW010000000
VERSION     DAPHDW010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5280
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.217"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            95..3532
                     /locus_tag="RH057_004267"
     CDS             95..3532
                     /locus_tag="RH057_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDV3130541.1"
                     /translation="MPLFRKNLITVSLLLSVFPGISWADRVDVPVYVEESLGPNWQSM
                     TIGTTSTSTSGMVPPVAHGTRSIAIGNNAVAGTEIEKPQVGEAIAIGSKANATKEQSV
                     AIGADTNATGWGSVVIGGDDIRPVHGKTYGRLTIPNAFLASTASGTGSVAIGSRTLSG
                     GELSLALGSIALAKGDASTAIGATAEAQDERSTAIGFNALSMARSALALGSESSATSE
                     SAMAFGSRAIAGKSSSIALGTDAVVSVGDNNVALGAGSQDKAATSVSSASVRSSDGRL
                     VTYQGFAASDPSSVISVGAQGKERQIVNVAAGAVTENSTDVINGSQLYSVAKSLQDDI
                     NAVTSAGFALKDQGNNEVRQVSGSAIQITGDDNITTTATDSDDNKSLKISLNDDLTVG
                     HDKAGSITVNNAAGNSSISLNGGDGTISLTSPAASGDTSTTATLGVTTGQGGLTDADG
                     TTKTRLIYTPDGSSEPEELASMNDGLSFVGDDGNVINKKLNETLSVTGGAGADAEMTD
                     NNIRVDNREGGLRVRMTRNLRDLASAVFGNGDNITTITDNGMSVTTGTGDNVRTVSLG
                     KEGLNNGGNIITHVASGLNGKQLSEADGDTLTNAANIGDLKSALQDTTSALVNSGYGV
                     QADDGNKVTRPLGEHVSIAGDGRNITTMVQDGKLLVQLSEHIDTGKNGSVTVGDTVIT
                     DEGLSITGGPSVTKSGINAGDQVISGVQDGVDAHDAVNVSQLNQQINNATAATTWSLK
                     TESSDASVAVSSQTVEVKHGLNTRVSAVQTDDKGNYSYEIDVTGIPVEYTDSKGNPLV
                     NIGGKFYTRTEDPDSGNITLSPAEPARVRISSDQPLVLTSVADGAVTPESVDAVNGGQ
                     LASAGNIVAGKNVVWNNGKATLSPDTFSELTTADGSNRTTTGTIQTVPDNVADAVNML
                     NREGTKYFKASSSGPAAKAEGRNSVAIGQSAISRAENSIAMGNGAEVSGDAAAGSVAI
                     GSGSRAGRAHTGNYSLNGQVVAGRTGSNTPVMSFGQAGQERQLQHVAPGVLSAESTDA
                     VNGSQLYAVHRQTEGNTQAINNLGKKFDHLNDRVDELNRDIRGVGASAAAMSGIPQAY
                     LPGKSLMGLGVGGYGGESAVAIGVSRISDNGKLIMKMNAGQNSRGNFSVGAGVGWQW"
     gene            3553..4332
                     /locus_tag="RH057_004268"
     CDS             3553..4332
                     /locus_tag="RH057_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000011714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV3130542.1"
                     /translation="MSFYKNILSGLMAVILTGCGNLSNVSPEGTTDEPVWPSPDRTMF
                     SHSGTQKGSWPVPEKLRLIKAGMNKDQIYQLAGRPHFGEGIAGVREWDYLFNFTGPDG
                     DYVCQFKILFDRHMDARSFFWKPENCSQEQEVKRYERTLQGDILFDHDSSVLTEKGRE
                     QLMIVAEELRKEGGRRFIIEGHTDHTGSEPYNNALSEKRAGTVKGWFISQGFDAGNIE
                     IRGKGDKEPLVVCENTGDEVMKECLRPNRRVVITAEEEGDR"
     gene            4422..4601
                     /gene="tnpB"
                     /locus_tag="RH057_004269"
                     /pseudo
     CDS             4422..4601
                     /gene="tnpB"
                     /locus_tag="RH057_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309368.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            4606..4856
                     /locus_tag="RH057_004270"
                     /pseudo
     CDS             4606..4856
                     /locus_tag="RH057_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(4995..5167)
                     /locus_tag="RH057_004271"
                     /pseudo
     CDS             complement(4995..5167)
                     /locus_tag="RH057_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT         1423 a         1189 c         1465 g         1203 t
ORIGIN      
        1 gcgaggagat aagacataat ataaactttt taccctctgt ttattcaaaa atcaatatat
       61 agtctgtaac accattgtta tttacgggtg atgtatgcct ttattcagaa aaaacctgat
      121 tacagtttct ctcctgctgt cagttttccc cgggatatcc tgggcagatc gcgttgatgt
      181 tcctgtatat gttgaagaat ctctggggcc aaactggcag agtatgacca ttggaaccac
      241 atccacaagc acatccggaa tggttcctcc ggtagcccac gggacccgtt caattgccat
      301 tggaaataac gctgttgctg gcactgaaat agaaaagccc caggtgggtg aagcgatcgc
      361 cattggctcc aaggcaaatg ctacaaaaga gcagtctgtg gcaattggtg ctgacaccaa
      421 cgccactggc tgggggagcg tcgtcatcgg gggcgacgat atacggcccg tacacggaaa
      481 aacttatggg cgcctgacca tcccgaatgc ctttctggct tcaacagcct caggaacagg
      541 ttctgttgcc attggcagcc gcacgctttc cggtggggag ttgtcgctgg ctctgggctc
      601 catcgccctt gcgaaaggtg atgcctccac ggctatcggt gccaccgctg aagcacagga
      661 tgaacgttcc accgccattg ggtttaacgc cctgtccatg gccagaagtg cactggctct
      721 tggcagtgaa tcatctgcaa cctccgagag tgccatggct ttcggcagca gggccattgc
      781 cggaaaatca tcttccattg cattaggcac tgatgcagtg gtttcggttg gcgacaataa
      841 cgttgccctg ggggccgggt cacaggataa agcggccaca agtgtgagca gtgccagtgt
      901 tcgctcatcg gatgggcgtc tggtcaccta tcagggcttt gccgccagtg atccatcctc
      961 tgttatttct gttggcgctc aggggaagga acgacagatt gtgaatgtcg cagccggtgc
     1021 cgtaacagag aacagtaccg atgtcattaa cggcagtcag ttgtattctg tggccaaatc
     1081 ccttcaggat gacataaatg ctgtcactag tgccggattt gcactgaaag accagggaaa
     1141 taatgaggtc agacaggtgt caggcagtgc aattcagatc accggtgatg acaatatcac
     1201 caccaccgcg acggacagtg atgataataa atcacttaag atttccctca atgatgacct
     1261 tacagtgggg catgacaaag cggggagtat aaccgtcaat aatgctgcag gtaacagcag
     1321 tatcagcctg aacggagggg acgggacaat ttctctgacc tcacccgcag cttccggtga
     1381 tacatccacc acagcaacac ttggtgtgac cacggggcag ggggggctga cggacgcaga
     1441 cggcaccacg aaaacccgtc tgatctatac acctgacggg agcagtgaac cggaagaact
     1501 ggcctccatg aatgacggtc tctcttttgt cggtgatgac ggaaatgtga ttaataaaaa
     1561 actgaatgaa acactgtcag tgacgggggg agccggcgca gatgctgaaa tgaccgacaa
     1621 caacattcgt gttgacaacc gggagggggg actcagggtc cgtatgaccc gtaatcttcg
     1681 tgatcttgcc agtgcggtct tcggaaacgg agataacatt acaacgataa ctgacaacgg
     1741 tatgtcggtg acaaccggta ccggagataa cgtgcgtacc gtttctcttg gtaaagaggg
     1801 gcttaataat ggcgggaata ttatcactca tgtggccagc ggactgaacg ggaaacaact
     1861 gtctgaagct gacggcgaca cactgacaaa tgcagccaat attggtgatc tgaaatcagc
     1921 cctgcaggac acaacgtcag cactggtgaa cagtggttac ggagtgcagg ctgatgacgg
     1981 aaacaaagtc acccgtccac tgggagaaca tgtcagtatt gccggtgatg gccgtaacat
     2041 cacgaccatg gttcaggacg gaaaactgct ggttcagctt tcagaacata ttgataccgg
     2101 taaaaatggc agtgtgacag tgggcgatac ggtgataaca gatgaaggac tgagcattac
     2161 aggcggacca tctgtcacaa aaagcgggat taatgcaggc gatcaggtta tttctggtgt
     2221 acaggatggc gttgatgctc atgatgccgt caatgtatct cagttaaacc agcaaattaa
     2281 taatgccact gctgcaacaa cctggtctct gaaaacggaa agcagcgacg cctctgttgc
     2341 agtatcgtct cagactgtgg aagtcaaaca tggcctgaac acacgtgtat cggctgtaca
     2401 gactgatgac aaagggaatt acagctatga aattgatgtg accggtattc ccgtggaata
     2461 taccgacagc aaaggcaacc cgctggtgaa tattggcggc aagttttata ccaggactga
     2521 agatcctgac agtggcaaca tcaccctttc tccggcagag ccggccaggg ttcgtatcag
     2581 cagtgatcag cccttggtcc tgacaagcgt ggcggacggt gcagtaactc cggagtctgt
     2641 ggatgcggta aacggaggac aactggcgtc agcagggaat attgttgccg gcaaaaatgt
     2701 ggtatggaat aacggaaaag cgaccctttc accggatacg ttcagtgaac tgaccactgc
     2761 cgacggaagc aaccgtacca cgacaggtac catacagact gtaccggata atgtggcaga
     2821 tgctgtcaat atgctgaacc gtgaagggac aaaatatttc aaggcatcct catcaggacc
     2881 ggcggcaaaa gcagaaggca ggaacagcgt ggccatagga caaagtgcca tttcccgtgc
     2941 tgaaaacagc attgcaatgg ggaatggtgc agaagtcagt ggtgatgcgg ctgccggctc
     3001 tgttgccatt ggcagtggct cacgggccgg acgggcacac accggtaatt attcgctgaa
     3061 tggtcaggtt gtggccgggc gtacaggaag taataccccg gtcatgtcat tcgggcaggc
     3121 cggtcaggaa cgccagcttc agcatgttgc accaggggta ttatccgcag aatcaacaga
     3181 tgccgtgaac ggaagccagt tatatgccgt tcaccgccag actgaaggga acactcaggc
     3241 aatcaataat ctcgggaaga aatttgatca tctgaatgac agagtggatg agctgaaccg
     3301 ggatattcgc ggagtcggcg catcagcagc tgcgatgagc ggtattcctc aggcttatct
     3361 gccggggaaa agtctgatgg ggcttggtgt tggtgggtac ggtggtgaaa gtgccgtggc
     3421 aataggggta tcccgtatat cagataacgg gaaacttatt atgaaaatga atgccgggca
     3481 aaattcacgc ggaaactttt ctgtgggagc aggtgtgggc tggcagtggt aacccggggt
     3541 aaggagtata agatgtcatt ttataaaaat atattatcag gtctgatggc ggttattctg
     3601 accgggtgcg gtaacctcag taatgtttca ccggaaggaa caactgacga acctgtgtgg
     3661 ccttctcctg acaggacaat gttcagtcac agcggaaccc agaaaggctc ctggcctgta
     3721 ccggagaagc tgcgtctgat aaaagccgga atgaataagg atcagatata tcagttggca
     3781 ggccgtcctc attttggtga agggatagcg ggagtgaggg aatgggatta cctctttaat
     3841 ttcaccgggc ctgacggaga ttatgtctgc cagttcaaga tccttttcga caggcacatg
     3901 gatgcaagat catttttctg gaaaccggaa aactgcagtc aggaacagga agtaaaaaga
     3961 tatgagagaa cattacaggg ggatattctg ttcgatcatg attcatcagt gctgacagaa
     4021 aaaggacgtg aacaactgat gatcgttgca gaagaactca ggaaggaagg gggcaggcgt
     4081 ttcattatag aaggtcatac agatcatacg gggtcagaac catataataa cgcactgtca
     4141 gagaaacggg ccggcacagt caaagggtgg tttataagcc agggttttga tgcaggaaat
     4201 atagagatcc ggggtaaagg tgacaaggaa cctctggttg tctgtgaaaa tacgggggat
     4261 gaggttatga aggagtgtct tcgtccaaac cgaagagtgg tgattactgc tgaggaggaa
     4321 ggagacaggt aaatagatct gatatacctg agcttgttat ttatatgctt ttatttttcg
     4381 gggcgcgtta gcgccctttt tattcttacc cttaaccatc tatgggggat ggtggtgatt
     4441 gccaatgcaa gaatatgcgc cggggcatcg acacactgat acagtacgtc cagaacgaac
     4501 ttaacgctgc atggcatgat ggtgctgctt ttgtcttcac caataaagcc cgctcccgca
     4561 taaaagttct ccgttgggat aaatatgatg tatggctgtg taaccgtgaa gaaaatcgcc
     4621 gggttgtacc gtcttgataa gaagatcagt agccgcctcg tggaaaaatc cgccagtggc
     4681 aacagcgtta tgcccgtccg ctactggaag atctgtggtc gtggttcgag gagcagtaac
     4741 cgaaatgttc ccctggcagg gcattacaca aagccattgt ctatgcgctg tctcatcgcg
     4801 tggaactgag ccgcttcctg gaagatggcg cggtgccgct agataataat gtgtgttaat
     4861 gggccatcaa aaacgtggtc ctgggcagaa ttaccatact cagtacttca aggcctgtcg
     4921 gcgtttatcc cgcacaggac ttcaacaccc gatcagggtc acctccgtcg cctgatcagg
     4981 ttacaatcgt cgtgtcaaga tgagatgcta ggaagcatac catcaacacg gttggaaggc
     5041 cattccggca atacgctcag gatatggcgc agatacgttt ccggatcgat accgttcagc
     5101 cggcaggtgc cgatcagtcc atgcacggta caccacgctt tccactgtaa tagttgccga
     5161 agaacacagt tatcaaatac atctggatta cttcaataca caaaaataat gaattttatg
     5221 tttgttcgca ttactgtgaa agaccgagag ttattgggta gtcttatttc atggaaaata
//
LOCUS       DAPHDW010000215         5192 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.218, whole genome shotgun sequence.
ACCESSION   DAPHDW010000215 DAPHDW010000000
VERSION     DAPHDW010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.218"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..1071)
                     /locus_tag="RH057_004272"
                     /pseudo
     CDS             complement(1..1071)
                     /locus_tag="RH057_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959889.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
     gene            complement(1090..1725)
                     /gene="hxsA"
                     /locus_tag="RH057_004273"
     CDS             complement(1090..1725)
                     /gene="hxsA"
                     /locus_tag="RH057_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016236322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser repeat protein HxsA"
                     /protein_id="HDV3130543.1"
                     /translation="MKKFNFAALLPGFLALNNSVWASDSSTGASDLPGMTLNEHDLVI
                     APLNTEVPFYIAGHRSHSSHRSHSSHRSSSGGGYYGGSTPYYPKTYSSPSSSGSSTSG
                     TNSPSSSSSVRSLRSNDADASTTTNSAGMNGTTRASSGLASDTEKRKRLIMRVQFALL
                     DKGFYNGNIDGSMGPATRTAIKNYRVAYGLPTPVRETLDAQLLNSLNILAR"
     gene            complement(1722..2834)
                     /gene="hxsC"
                     /locus_tag="RH057_004274"
     CDS             complement(1722..2834)
                     /gene="hxsC"
                     /locus_tag="RH057_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012134292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsC"
                     /protein_id="HDV3130544.1"
                     /translation="MSEVLRNDIFRFTADLPVQQGFYRLCKSKPENPQFYLPNLLVSE
                     TQLDSRLPAYFADFVVSTDLFNSIEDGDIGIVNNGNMIRVILSRRANHNTVLVTERCN
                     NRCLFCSQPPKTGNDDWLLYQSALAIASFSLNGVVGVSGGEPLLYGEDFLQFLDFIIE
                     NSPETALHVLTNGRKFADVSFTQQMKERSEKLKITFGIPLYSSRSSVHDYLVGSEGAF
                     DETVMGLINAGNSGINIELRIIPTLANYMELDKIIEFAGRVFSNINQISLMGLESIGW
                     ARKNWSSIFIEHDSYSEKILSAIGTAQRSGITLTIFNYPLCHLPERAWGFATQSISDW
                     KNYYPKECDECTQKSSCAGYFSSSKGRFHQPPRPIL"
     gene            complement(2827..4215)
                     /gene="hxsB"
                     /locus_tag="RH057_004275"
     CDS             complement(2827..4215)
                     /gene="hxsB"
                     /locus_tag="RH057_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007705390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system radical SAM maturase HxsB"
                     /protein_id="HDV3130545.1"
                     /translation="MKLMPFNFDRMPDGRVFISNLAGFHHFVGEQEFLDLVNGHISQE
                     ESSSLESKLFICGDESSSVTPYALSSAFAKRLMNELAIRPIFMIVPTLRCDHTCKYCQ
                     VSRASVTADGYDLNPELIPQIVSTIKKLGTPPYKIEVQGGEPLLRFDLVQSIYHECEI
                     SLGSDAFEMVIATSLSLLDESILSWVKERNITFSVSLDGNEAVHNKNRILTDFQAHNK
                     AVTGIRKITEELGANRVATVTTVTKELTKEPASIVDAHLSLGLTDMFIRPVSPYGFAQ
                     KQSFTFSMPEYFAFYKELMQEVLIQDEKGIPVIEHSAAIHLKRIFNPCFSGYADLKSP
                     SGVVLNCILFNYDGKVYGSDESRMLQKVNPEADFSAGEFASLSFSSNEYYRSALSSSF
                     NFAMPGCDTCAYQPFCGADPCQNISVHGEPVGDKSRSTFCQYHKGMFRFLLNEISQDG
                     PMAKMLKGWAYV"
     gene            complement(4215..4487)
                     /gene="hxsD"
                     /locus_tag="RH057_004276"
     CDS             complement(4215..4487)
                     /gene="hxsD"
                     /locus_tag="RH057_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His-Xaa-Ser system protein HxsD"
                     /protein_id="HDV3130546.1"
                     /translation="MWEKILQKNLYSEWVIRNTLYWMTPVSPWKLEENTSSWIIFFEI
                     GGPECEFEFGRLLNDFSLREKLHHQTGQLRDAIISKVLRSIDDRLA"
     gene            4887..5120
                     /locus_tag="RH057_004277"
     CDS             4887..5120
                     /locus_tag="RH057_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
                     /protein_id="HDV3130547.1"
                     /translation="MAIPGNVWLYDDGGALIKGGCDVADREFSIEFNRFHHNLSIPTD
                     NATGKPTGTLQHSPMIIVKEFGYSSPYFYGGLN"
     gene            5078..>5192
                     /locus_tag="RH057_004278"
     CDS             5078..>5192
                     /locus_tag="RH057_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1493 family protein"
                     /protein_id="HDV3130548.1"
                     /translation="MRLFQPILLRWFKLKREDVKRTRKPLTVKIFAESAEAG"
BASE COUNT         1549 a         1088 c         1028 g         1527 t
ORIGIN      
        1 aatgaattat ttttttcatt tcatcatcaa tagtcgatac aaaattccta tctgcaacat
       61 accatataga gtctcgaaga atatagttct gatcaccttc ttttatctcg gcatagaggc
      121 acctatataa agaccatttt tttatcgatt caaaatcggc attcaaaaca tgcagataat
      181 ttttttgagt tcatcaacag ttacttcagt tttcttacca tcaaaataat cacaaacatg
      241 gtatattgat aaggtttcat gaatgaatct ttgtctctta tcaaagcaat agcctatttg
      301 attttcccag tctactattt ctggctctcc aagccataaa tcattgaagt ctttttcctt
      361 tatccgatca atcaaaagcg aatccaatat ctcaacttct tgttcatcag cctccttgat
      421 attgttaacc cattcgaatt cttcaggaag aggtaataag taaatagagt ctattttggt
      481 caatagtgct ggaatagcct tcaattcaat atccggcatg atcacaaaag catctttgcc
      541 agtaatttta cttcctaaaa tatcttctgt cgaagtgcct gtgagagttg tgagaatatc
      601 catctcagaa tctattttta ggttgtatat atcgacttct ttactgctct gactgcgagt
      661 taacaggttc gtttcgttgt gactgccttt atctaaactg cgaatttttt tatgttctat
      721 actattaaga gttgttttaa gcccaaatcc tttgacaatg ctgccatcat tgattaagtg
      781 atgaccggtt ccaaagggaa taagatatct ggaattgtta gcttcaataa ctaatactgc
      841 tcctgtgcta ctattcatac cgaagaaatc aggttctatg ccaggatttt ggtccataaa
      901 aaaggtggtc cattcaggag gttttgggtc catgtactcc tttacatata gatatgctcg
      961 tgcatcctcc atctcgaatt caacaggtct tttgaccctt tccaaattca aaacctgatc
     1021 aatatcatcg ctaaaccttt ttgttttata aatagagagt tttatcttca ttctatttta
     1081 cctaatattt taacgagcca gtatattcaa agagttcaat aactgagcat ctaatgtttc
     1141 tcttactgga gtaggaagcc catatgcaac tctgtaattt ttgatagcag ttcgtgtcgc
     1201 aggccccata cttccgtcta tattgccgtt ataaaaccct ttatccagta atgcgaactg
     1261 cactcgcatg atcaaacgct tacgcttttc agtatcagaa gccagcccac tacttgcacg
     1321 agtagttcca ttcattccag cagagtttgt ggtcgtagag gcatcggcgt cattagagcg
     1381 aagcgaacga acagatgaag aagacgatgg cgaatttgta cctgaagtgg atgagcctga
     1441 tgagcttggt gaactgtatg ttttaggata ataaggagtg cttccaccat agtacccacc
     1501 accagacgaa gatctatgag aactatggct tcgatgggag ctatgactac gatgccctgc
     1561 aatgtagaaa ggtacttccg tgttaagtgg agctataact aaatcatgct cattaagagt
     1621 cataccgggc aaatcacttg caccagtgga ggaatcactt gcccatacag aattattgag
     1681 tgctaaaaat cccggaagca gagcagcgaa attaaatttt ttcataaaat tggtctcggt
     1741 ggttgatgaa aacggccttt tgatgaactg aaataaccag cacaggaaga cttctgagta
     1801 cattcatcac attctttggg gtagtaattt ttccagtcag aaattgactg agtagcaaat
     1861 ccccaagctc tttcaggaag atggcacaat gggtaattga agattgttag ggtgatgcct
     1921 gacctttgtg ctgttcctat ggcagagaga attttctcac tatagctatc atgctcaatg
     1981 aagattgatg accagttttt acgtgcccag ccgatagatt ctaaccccat aagagaaatc
     2041 tggttgatat tggaaaacac gcgaccagcg aactctatga ttttgtccag ttccatatag
     2101 ttagccaatg tcggaatgat tcttaactca atgttgatcc ctgagttacc ggcattgata
     2161 agccccataa ctgtctcatc aaatgcccct tcgcttccaa ccagatagtc atgaactgac
     2221 gaccttgaag aataaagcgg aataccgaaa gttattttga gcttttcgct tcgctctttc
     2281 atctgttgag taaaactgac atctgcaaac tttcgtccat tggtcaaaac atgcaaagct
     2341 gtctcaggtg agttttcgat tatgaagtcc agaaattgca agaaatcttc gccatacagt
     2401 agaggttctc ccccactcac tcctactaca ccattcaggg agaaagaggc gatggcaagt
     2461 gctgactggt aaagaagcca gtcatcgttt cctgtcttag gaggctgaga acaaaaaagg
     2521 caacggttgt tgcacctttc agttaccaga acagtgttat ggtttgctct ccgtgacaaa
     2581 atcaccctaa tcatgttgcc attgttaaca atccctatat caccatcttc tatggaattg
     2641 aaaagatcag tgctcacaac gaagtctgca aaatatgcag gaagtctgga atcaagttga
     2701 gtttcactga ccagtaaatt tggcagatag aattgaggat tttcgggctt agatttgcac
     2761 aagcgataga aaccttgctg aactggcaga tctgccgtaa atctgaaaat gtcgtttctc
     2821 agcacctcag acataagccc accctttaag catctttgcc atcggtccat cctgagagat
     2881 ttcattcaga aggaagcgga acatgccttt gtgatactgg cagaaagtag aacgactctt
     2941 gtcaccaaca ggttcgccat gaacactaat gttctggcaa ggatctgctc cacagaaagg
     3001 ctggtatgca caggtgtcac aacctggcat ggcgaagtta aatgaagatg agagagcgga
     3061 acggtagtat tcgttgctac tgaatgagag tgaggcaaac tctcctgcac tgaaatctgc
     3121 ttccgggttt actttctgaa gcatacgact ttcatcacta ccgtagactt taccgtcata
     3181 attgaacagg atgcagttca gcacaactcc gcttggcgat tttagatctg cataaccact
     3241 gaagcatgga ttgaaaattc gcttgagatg aatagcagca gagtgctcaa tgacaggtat
     3301 tcctttttca tcctgaatca acacttcctg catcaactct ttataaaaag caaaatactc
     3361 tggcattgaa aaagtgaagc tctgcttctg agcaaaccca taaggactaa caggcctgat
     3421 aaacatgtct gtaagaccca gagacaaatg agcatctact atggaagctg gctctttagt
     3481 cagctcctta gtaactgtgg taaccgtagc tacccggttt gcgccaagtt cttctgtgat
     3541 tttccgaata ccagttactg ctttgttatg agcttgaaag tcagtaagga tgcgattctt
     3601 gttatggaca gcttcattcc catcgagcga gaccgagaag gtgatattcc gctctttaac
     3661 ccatgagaga atgctttcat cgagtaatga cagacttgta gcaatcacca tttcaaaagc
     3721 atcactaccc aatgaaatct cacactcgtg atagattgac tgaacaagat cgaacctgag
     3781 aagtggttca ccgccctgta cttctatctt gtagggaggt gtacctagtt tctttatggt
     3841 gctgacaatt tgcggtatca gttcaggatt gaggtcataa ccatcagcgg tgaccgatgc
     3901 cctactgacc tggcaatatt tgcaagtatg atcacaacga agagtaggta caatcatgaa
     3961 gattggcctg atcgccagtt cattcatgag tcgtttggca aaagccgaac ttagtgcata
     4021 aggggtaacg gaggaagatt catctccgca aatgaaaagt ttgctttcaa gcgatgagga
     4081 ctcttcttga ctgatatgac cgtttaccag atcgagaaat tcctgctcac ctacaaaatg
     4141 atgaaagcca gcgaggttac tgatgaatac ccggccgtca ggcatcctgt cgaagttaaa
     4201 aggcatgagt ttcattatgc aagcctgtca tctatgcttc ggagaacctt gctgataatg
     4261 gcatcacgca actgaccagt ttgatgatga agtttctcgc ggaggctaaa atcgtttaac
     4321 agccttccga attcaaactc acattcagga ccacctatct caaagaagat aatccacgag
     4381 gatgtgtttt cttcaagttt ccatggactt acaggagtca tccagtatag ggtgtttcgg
     4441 ataacccatt ctgaataaag atttttttgt aatatctttt cccacatcgc cattaccttc
     4501 caaacgaaat aataacggaa agaaacttat tccatttagg gatttttctt atagattaat
     4561 gatataacac ttttcaaaac atgttactga gtatgtcaaa attctgagat tcagttcaac
     4621 tagaaattaa tcgaatcacg cactcacatg atgcagaagt accatttttc aatggttaac
     4681 ctgttgaagt aaaggaacaa agagattact agctatgttc tagtttactg gtcgcgtact
     4741 attctgtgaa tgtcggtgtg attggtagaa gcagataact tactcttcca gggagtaaaa
     4801 aaaaaccaca cattggttat ttactcacct ccactcaata gatacactat ttaaagggat
     4861 ttgcaacgac ataaggaaaa aagaacatgg ctattccggg taatgtgtgg ctctatgacg
     4921 atggtggagc actaatcaaa ggaggttgtg atgttgcaga cagggaattc agtatagagt
     4981 ttaataggtt tcaccataat ctctccattc ctactgataa tgccactggc aagcctaccg
     5041 gcactcttca gcactcacca atgatcatcg ttaaagaatt cggctattcc agcccatact
     5101 tctacggtgg tttaaattaa aacgggagga tgtaaaacga acccgtaaac ctttaactgt
     5161 caaaatattt gcagaatcag cagaagctgg aa
//
LOCUS       DAPHDW010000216         5070 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.221, whole genome shotgun sequence.
ACCESSION   DAPHDW010000216 DAPHDW010000000
VERSION     DAPHDW010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5070
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.221"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            25..1359
                     /gene="garP"
                     /locus_tag="RH057_004279"
     CDS             25..1359
                     /gene="garP"
                     /locus_tag="RH057_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HDV3130549.1"
                     /translation="MILDTVDEKKKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVIGFVLTALWIKLIHNPTDHPRMSAEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            1375..2145
                     /gene="garL"
                     /locus_tag="RH057_004280"
     CDS             1375..2145
                     /gene="garL"
                     /locus_tag="RH057_004280"
                     /EC_number="4.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
                     /protein_id="HDV3130550.1"
                     /translation="MNNDVFPNKFKAALAAKQVQIGCWSALSNPISTEVLGLAGFDWL
                     VLDGEHAPNDISTFIPQLMALKGSASAPVVRVPTNEPVIIKRLLDIGFYNFLIPFVET
                     KEEAEQAVASTRYPPEGIRGVSVSHRANMFGTVADYFAQSNKNITILVQIESQQGVDN
                     VDAIAATEGVDGIFVGPSDLAAALGHLGNASHPDVQKAIQHIFNRASAHGKPSGILAP
                     VEADARRYLEWGATFVAVGSDLGVFRSATQKLADTFKK"
     gene            2175..3065
                     /gene="garR"
                     /locus_tag="RH057_004281"
     CDS             2175..3065
                     /gene="garR"
                     /locus_tag="RH057_004281"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HDV3130551.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGEIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALACYYEKLAKVEVTR"
     gene            3162..4307
                     /gene="garK"
                     /locus_tag="RH057_004282"
     CDS             3162..4307
                     /gene="garK"
                     /locus_tag="RH057_004282"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HDV3130552.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNAG"
     gene            4340..4716
                     /gene="rnpB"
                     /locus_tag="RH057_004283"
     ncRNA           4340..4716
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="RH057_004283"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
BASE COUNT         1179 a         1217 c         1462 g         1212 t
ORIGIN      
        1 gcataagtac ccgaggtaaa taaaatgatt ctggacaccg ttgacgaaaa aaagaaaggc
       61 gtgcataccc gctatttaat attactgatt atttttattg ttaccgccgt taactacgcc
      121 gatcgtgcaa cgctgtctat tgctggtacc gaagtggcaa aagagttgca gttaagtgcg
      181 gtttcgatgg gttacatctt ttctgctttt ggctgggcct acttgctgat gcaaatcccc
      241 ggcggctggc tgcttgataa gtttggctcg aaaaaagttt atacctatag cctctttttc
      301 tggtcgctat tcactttcct gcaaggcttt gttgatatgt tcccgctggc ctgggcaggg
      361 atctccatgt tctttatgcg ctttatgctc ggcttctcgg aagcgccatc attcccggcg
      421 aacgcccgta ttgtcgccgc ctggttcccg acgaaagaac gtggtactgc ctccgccatc
      481 tttaactcgg cgcaatattt ctcgctggcg ctcttttcgc ctctgcttgg ctggctgact
      541 ttcgcctggg gctgggagca cgtctttacc gttatggggg tgattggttt tgtgctgacg
      601 gcgctgtgga tcaaattgat tcataacccg acagatcacc cacgtatgtc tgcggaagag
      661 ctgaagttta tctctgaaaa tggcgcggtg gtcgatatgg accacaaaaa gccgggcagc
      721 gcggcagcaa gcggtcccaa actgcattac atcaagcaat tgctctctaa ccgcatgatg
      781 ttgggcgtat ttttcggaca atattttatc aacaccatca cctggttctt cctcacctgg
      841 ttcccgattt atctggtgca ggaaaaaggc atgtcgattc tgaaagtggg tctggtcgcc
      901 tcgattccgg cactgtgtgg ttttgcgggc ggcgtgctgg gaggtgtctt ctcggattat
      961 ctgatcaaac gcggtttatc cctgaccctg gcacgtaagc taccgattgt gctgggaatg
     1021 ttgctggctt ccaccatcat cttatgtaac tacaccaaca acaccacgct ggtggtcatg
     1081 ctaatggcgc tggctttttt tggcaaagga tttggtgctc tgggctggcc ggtgatttct
     1141 gacaccgcgc cgaaagagat tgttggcctc tgtggcggcg tctttaacgt ctttggcaat
     1201 gttgcctcca ttgtcactcc actggtgatt ggctacctgg taagtgaact gcactccttc
     1261 aatgcagcac tggttttcgt gggatgttca gcgctgatgg cgatggtctg ctacctcttc
     1321 gtagttggcg acattaaacg tatggaattg cagaaataag caaaggtata agcgatgaat
     1381 aacgatgttt tcccgaataa attcaaagcc gcactggctg cgaaacaggt acaaattggt
     1441 tgctggtcag cactctctaa cccgattagc actgaagttc ttggtttggc tgggtttgac
     1501 tggctggtgc tggatggcga acatgcgcca aacgatatct ccacgtttat tccgcagtta
     1561 atggccctga aaggcagcgc cagcgcgcca gtagtgcgag tgccgaccaa cgagccggta
     1621 attattaagc gtcttctgga tatcggtttc tataacttcc tgattccttt tgtagaaaca
     1681 aaagaggaag cagagcaggc ggtggcatca acccgttacc caccggaagg cattcgcggc
     1741 gtctccgttt ctcaccgcgc caatatgttt ggcaccgtgg cggattattt cgctcagtcg
     1801 aacaagaaca tcaccattct ggtccagata gaaagtcagc agggcgtaga taacgtcgat
     1861 gccattgccg ctaccgaagg tgtagacggt atcttcgtcg gccccagcga tctggctgcg
     1921 gcattaggcc atctcggcaa tgcctcgcac ccggatgtac aaaaagcaat tcagcacatt
     1981 tttaaccgtg ccagtgcgca cggcaaaccc agcggtattc tcgcgccggt cgaagccgat
     2041 gcgcgtcgtt atctggaatg gggcgcgacg tttgtggctg tcggcagcga tcttggcgtc
     2101 ttccgctctg ccactcagaa actggctgat acctttaaaa aataaccacc accgcaacaa
     2161 gagagatgat tgatatgact atgaaagttg gttttattgg cctggggatt atgggtaaac
     2221 caatgagtaa aaaccttctg aaagcaggtt actcgctggt ggttgctgac cgtaacccag
     2281 aagctattgc tgacgtgatt gctgcaggtg cagaaacagc gtctacggct aaagcgatcg
     2341 ctgaacagtg cgacgtcatc ataaccatgc tgccaaactc ccctcatgtg aaagaggtgg
     2401 cgctgggtga gaatggcatc attgaaggcg cgaagccagg tacggtattg atcgatatga
     2461 gttctatcgc accgctggca agccgtgaaa tcagcgaagc gctgaaagcg aaaggcattg
     2521 atatgctgga tgctccggtg agcggcggtg aaccgaaagc catcgacggt acgctgtcag
     2581 tgatggtggg cggcgacaag gctattttcg acaaatacta tgatttgatg aaagcgatgg
     2641 cgggttccgt ggtgcatacc ggggaaatcg gtgcaggtaa cgtcaccaaa ctggcaaatc
     2701 aggtcattgt ggcgctgaat attgccgcga tgtcagaagc gctaacgctg gcaaccaaag
     2761 cgggcgttaa cccggacctg gtgtatcagg caattcgcgg tggactggcg ggcagtaccg
     2821 tgctggatgc caaagcgccg atggtgatgg accgcaactt caagccgggc ttccgtattg
     2881 atctgcatat taaggatctg gcaaatgcgc tggatacttc ccacggcgtc ggcgcgcaac
     2941 tgccgctcac agctgcggtt atggagatga tgcaggcact gcgagcagat ggtttaggaa
     3001 cggcggatca tagcgccctg gcgtgctact acgaaaaact ggcgaaagtc gaagttactc
     3061 gttaatgacg tcgtgcccgg atggcgtatt gcaatccggg cctggaatcc aggccgaata
     3121 agagaaacgc cctccggcgt catgtggtaa caggcatagg tatgaaaatc gtaatcgccc
     3181 cagactctta taaagaaagt ttatctgcca gcgaggttgc gcaggcgata gaaaaaggat
     3241 ttcgggaaat ttttcctgat gcacagtacg tttctattcc ggttgccgac ggtggcgaag
     3301 gaacggtgga agcgatgatt gcagccaccc agggttctga acgtcacgcc tgggttacag
     3361 ggccgctggg cgagaaagtg aatgccagtt gggggatctc cggcgatggc aaaaccgcgt
     3421 ttattgaaat ggcggcggcc agtgggctgg agctggtacc tgcggaaaaa cgcgatccac
     3481 tcgtgaccac ttcacgcggt acaggcgagt taatcctgca ggcgctggag agcggcgcga
     3541 caaacattat tatcggcatt ggcggtagcg ctacaaatga tggcggcgca ggcatggtac
     3601 aggcgctggg ggcgaaatta tgcgacgcca acggcaatga aattggtttt ggcggcggta
     3661 gtcttaatac tctgaatgat attgatattt ccggcctcga tccgcgctta aaagattgcg
     3721 tcattcgcgt cgcttgtgat gtcaccaatc cgctggtggg cgataacggc gcatcgcgca
     3781 tctttggccc acaaaaggga gccagtgaag cgatgattgt tgagctggac aataacctct
     3841 ctcactatgc cgatgtcatt aaaaaagcgc tgcatgttga tgtgaaagat gtccccggcg
     3901 caggagctgc gggcggtatg ggtgcggcgc tgatggcgtt tcttggtgcg gaactgaaaa
     3961 gtggtattga aatcgtcact acggcgctga atctggagga acatattcac gattgtacgc
     4021 tggtgatcac cggtgaaggg cgtattgaca gccagagtat tcacggcaag gtaccgattg
     4081 gcgttgcaaa cgtggcgaag aagtaccata aaccggtgat tggcattgcg ggtagcctga
     4141 ccgatgatgt tggcgttgta catcagcatg gcatcgatgc ggtcttcagc gtattgacca
     4201 gcataggtac gttggacgaa gcattccgcg gggcttatga caatatctgc cgtgcttcac
     4261 gtaatatcgc cgcgacactg gcgattggaa tgcgcaacgc ggggtgacaa gggcgcgcaa
     4321 accctctata ctgcgcgccg aagctgacca gacagtcgcc gcttcgtcgt cgtcctcttc
     4381 gggggagacg ggcggagggg aggaaagtcc gggctccata gggcagggtg ccaggtaacg
     4441 cctggggggg aaacccacga ccagtgcaac agagagcaaa ccgccgatgg cccgcgcaag
     4501 cgggatcagg taagggtgaa agggtgcggt aagagcgcac cgcgcggctg gtaacagtcc
     4561 gtggcacggt aaactccacc cggagcaagg ccaaataggg gttcataagg tacggcccgt
     4621 actgaacccg ggtaggctgc ttgagccagt gagcgattgc tggcctagat gaatgactgt
     4681 ccacgacaga acccggctta tcggtcagtt tcacctgatt taacgaaaac ccgccagcgt
     4741 aaaccggcgg gtttttgctt ttggaggggc agaaagatga atgactgtcc acgacgctat
     4801 acccaaaaga aagcggctta tcggtcagtt tcacctgatg tacgtaataa accgttccgg
     4861 cgggtttcag attgttgagt gcgctttatt catgccggat gcggcgtgaa cgccttatcc
     4921 tgcctacaaa agcacgcaaa ttcaatatat tgcagagatg gcgtaggcct gataagcgta
     4981 gcgcatcagg catttttgcg gttgccatca gtttcaaacc cgcgtcagtg agtttttgct
     5041 tttggtgcac agggtaagat gaataaatat
//
LOCUS       DAPHDW010000217         4997 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.222, whole genome shotgun sequence.
ACCESSION   DAPHDW010000217 DAPHDW010000000
VERSION     DAPHDW010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.222"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(20..478)
                     /locus_tag="RH057_004284"
     CDS             complement(20..478)
                     /locus_tag="RH057_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HDV3130553.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNPRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            598..1407
                     /gene="proC"
                     /locus_tag="RH057_004285"
     CDS             598..1407
                     /gene="proC"
                     /locus_tag="RH057_004285"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HDV3130554.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(1424..2539)
                     /gene="adrA"
                     /locus_tag="RH057_004286"
                     /gene_synonym="dgcC"
     CDS             complement(1424..2539)
                     /gene="adrA"
                     /locus_tag="RH057_004286"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HDV3130555.1"
                     /translation="MFPKIMNDENFFKKAAAHGEEPPLTPQNEHQRSGLRFARRVRLP
                     RAVGLAGMFLPIASTLVSHPPPGWWWLVLVGWAFVWPHLAWQIASRAVDPLSREIYNL
                     KTDAVLAGMWVGVMGVNVLPSTAMLMIMCLNLMGAGGPRLFVAGLVLMVVSCLVTLEL
                     TGITVSFNSAPLEWWLSLPIIVVYPLLFGWVSYQTATKLAEHKRRLQVMSTRDGMTGV
                     YNRRHWETMLRNEFDNCRRHNRDATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQ
                     ITLRGSDVIGRFGGDEFAVIMSGTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVG
                     VAPLNPQMSHYREWLKSADLALYKAKKAGRNRTEVAA"
     gene            complement(2641..2961)
                     /gene="psiF"
                     /locus_tag="RH057_004287"
     CDS             complement(2641..2961)
                     /gene="psiF"
                     /locus_tag="RH057_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HDV3130556.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQSLKGDDRNKFMSACL
                     KKAA"
     gene            complement(3080..4495)
                     /gene="phoA"
                     /locus_tag="RH057_004288"
     CDS             complement(3080..4495)
                     /gene="phoA"
                     /locus_tag="RH057_004288"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HDV3130557.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTGVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVSDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTCTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKDGNTLVIVTADH
                     AHASQIVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(4596..4856)
                     /gene="iraP"
                     /locus_tag="RH057_004289"
     CDS             complement(4596..4856)
                     /gene="iraP"
                     /locus_tag="RH057_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HDV3130558.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPRQ"
BASE COUNT         1167 a         1349 c         1229 g         1252 t
ORIGIN      
        1 tgtaaataat aaattacatt tagccacgac tacgttgcac ttccagccac cacttctcca
       61 gctccgcggc aaaggcctgg cggtcacgtt gtgaaaggct atctggtccg ccggtctgga
      121 tcccactggc acgtaaggta tccataaaat cgcgcatcgt caggcgctca cgaatggtcg
      181 ctggcgtgta acgttcgccg cgcggattaa gcgccgctgc gcctttctcg atggcttcag
      241 cagccaaagg tatatctgcg gtgatcacca aatcgcccgc ttcacactgc cggacaattt
      301 cgttatcggc aacgtcgaaa cctgccgcga cgcgcagcgt acgaataaat cgcgatggcg
      361 gcacgcgtaa actctggttt gctaccagta ccagcggcat ctgcatacgt tccgccgcgc
      421 gatacaaaat ctctttaatt acattgggac acgcgtcggc atccacccaa attgtcataa
      481 agtcatcctt tgttgggtaa tcctctattg tgtcgcgctt ttgccttccg gcatagttct
      541 gtttatgctt ctgccagcga ttatcaaaac aatgaatttc acggcaggag tgaggcaatg
      601 gaaaagaaaa tcggttttat tggctgcggc aatatgggaa aagccattct cggcggtctg
      661 attgccagcg gtcaggtgct tccagggcaa atctgggtat acaccccctc cccggataaa
      721 gtcgccgccc tgcatgacca gttcggcatc aacgccgcag aatcggcgca agaagtggcg
      781 caaatcgccg acatcatttt tgctgccgtt aaacctggca tcatgattaa agtgcttagc
      841 gaaatcacct ccagcctgaa taaagactct ctggtcgttt ctattgctgc aggtgtcacg
      901 ctcgaccagc ttgcccgcgc gctgggccat gaccggaaaa ttatccgcgc catgccgaac
      961 actcccgcgc tggttaatgc cgggatgacc tccgtaacgc caaacgcgct ggtaacccca
     1021 gaagataccg ctgatgtgct gaatattttc cgctgctttg gcgaagcgga agtaattgct
     1081 gagccgatga tccacccggt ggtcggtgtg agcggttctt cgccagccta cgtatttatg
     1141 tttatcgaag cgatggccga cgccgccgtg ctgggcggga tgccacgcgc ccaggcgtat
     1201 aaatttgccg ctcaggcggt aatgggttcc gcaaaaatgg tgctggaaac gggagaacat
     1261 ccgggggcac tgaaagatat ggtctgctca ccgggaggca ccaccattga agcggtacgc
     1321 gtactggaag agaaaggctt ccgtgccgca gtgatcgaag cgatgacgaa gtgtatggaa
     1381 aaatcagaaa aactcagcaa atcctgatga ctttcgccgg acgtcaggcc gccacttcgg
     1441 tgcggttacg tccggctttc tttgctttgt aaagcgccaa atctgccgat ttcaaccact
     1501 cacgatagtg actcatttgt gggttcagcg gcgcaacccc cacactaatc cgtaaagtta
     1561 cctgtggcgt attcggcaag cgtaacgtgt ttagcccttc acgcacccgt aacatggcgg
     1621 taatggcgct ctcagctggc gtaccggaca tgattactgc aaactcatcg ccgccaaacc
     1681 gaccaatcac atcgctaccg cgcagggtaa tttgtaactg tcgggtaagc gccacaatcg
     1741 cttcatcgcc cacatcatga ccccaggtat cgttgatgct cttgaaatgg tcgatatcga
     1801 taatcagtaa cgttgcatcg cgattatgcc gccgacagtt atcaaattca ttgcgtaaca
     1861 tagtttccca atgacgtcgg ttatacacgc ccgtcatgcc gtcgcgggta ctcatgacct
     1921 gcaacctgcg tttatgttcc gccagtttgg ttgccgtctg gtagctgacc cagccaaaca
     1981 gcagaggata aacgacaata atgggaaggg agagccacca ttccagcggc gcactattga
     2041 acgacacggt aatgcccgta agctcgaggg tgacaaggca ggaaaccacc atcaacacca
     2101 gacccgcgac aaacagacgg gggccgcctg cccccatcaa attcagacac ataatcatca
     2161 acatagcggt ggaaggcagc acgtttacgc ccattacgcc tacccacatt cccgctaata
     2221 ctgcatcggt ttttaagttg taaatttccc ggctaagcgg atcgacggcc ctgctcgcta
     2281 tctgccaggc taaatgcggc cagacgaacg cccagccgac caacaccagc caccaccagc
     2341 ccggcggcgg gtgcgaaacc agcgttgaag caatcggtaa gaacatgcca gccaggccaa
     2401 ccgcacgggg tagtctgacg cgacgggcga agcgcagccc ggaccgctga tgttcgtttt
     2461 gaggagttaa aggaggttcc tccccatgcg ccgccgcttt tttgaaaaag ttttcatcat
     2521 tcattatttt tgggaacatt cagaaacaat tttcccaaat tatagagacg gggctaaagg
     2581 cagaagtatg aaatttcggg tgagccactt tcgtagctca cccgttagca ctatcaggta
     2641 tcaggcggct ttcttgaggc aggcactcat aaacttatta cgatcatcac ctttcagaga
     2701 ttgttgcgtt gcttgattat tacattcgcg catcttttgc tgctgtggcg tcaaactttt
     2761 ttcaccaggc gcagacttgc tgttcttcag gcaatcactc atgtaggtct tacgagcatc
     2821 ccctttcaac gcctgcgccg tcgcctgctg attacaggat gtcatacgct gttgttgtgg
     2881 ggttaaggtt ctctcggcag cgccgacggt ggttaaaaaa accagaccaa aaagcaaggt
     2941 aaccagtaat gttattttca tagcaccatc cctcttcatg ttttaaccat gagcgtatgc
     3001 gcccgtgatc tgccattaag tctggttgct aacagcaaaa aaaccacccg gcagcgaaaa
     3061 ttcactgccg ggcgcggttt tatttcagcc ccagggcggc tttcatggtg tagaagagat
     3121 cggtctggtc ggtcagtcca acaacattgg cggcatgcgg gccatacgcc gcaatacgca
     3181 actgactgcc ggtatgttct tgtgaatcct cttcggagtt cccgtagctc atcaccatca
     3241 ctgcgccatc tttggtattt agcgcctggg tgaggcccgg agctttggta tccggcgcga
     3301 caatctggct ggcgtgggcg tgatcagcgg tgactatgac cagcgtattg ccatcctttt
     3361 tagcgaattc cagcgcccgt tgtacggctt catcgagatc gaccgtctcg ccaatttgcc
     3421 cacaaggatt cgcagcgtga tcctgtttat cgattgacgc accttcaact tgcaggaaaa
     3481 atcctttctc atttttactc aacaattcaa tggctttgtc ggtcatctgc gccagggttg
     3541 gtacactgtc attacgttgc ggattaggcg tacaggtgac tgcgggctta tcgatattgc
     3601 cgtggtacgt tgctttcggt cctagccagc gcactggcat attgccgtca gcaaacagtc
     3661 caagcagagg tttttgctga ttcgcttccg tcaccgaatt cagtgaggca gcatcgctga
     3721 ccaactgata accacgcgcc tgtgcctgtt cacgcagcgt ttttccctgc cattcaccgg
     3781 cggttgccgt ttcagcaaag gtttttgcgc caccgccaag cgtaacgtcg gcacgagcgt
     3841 taagcagctg ttcggtaatc gatccttttc cgcctttttc cagagcgtta cccggacatt
     3901 tttcactggt cgcgctcgga ccgtagcatt tgcgcgaagt cacatgtgcc accagcgcag
     3961 cgggcgtggc atcctgcaac tctgcggtag aaacgttacc ggtcgccaga cctgcggctt
     4021 ttgccatttc cagaatcgtt gggtgatctt tttcgtgaat atcgacgccc agcgcgccgt
     4081 tataggtttt gacaccggtt gaccaggcgg ttgctgatgc agccgagtcg gtgacgtagt
     4141 ccggtttgcc ggttttttta ttcagcgcat agtgagtgta ttgcccggta agcggtaagg
     4201 catcgatacc tttaaaaaag ccgcccgcac cttcggcata attacgtgcg gcagtaattt
     4261 ccgaatcccc catcccatcg ccaatcagca aaataatatt ttttgcaggt ttatcgctaa
     4321 gagaatcacg cagagcggcg gtctgatcac ccgttaagcg gcgagcaccg ccgggtgcag
     4381 taatatcgcc ctgagcagcc cggttttcca gaacaggcat ttctggtgtc cgggcttttg
     4441 tcacaggggt aaacagtaac ggtaagagtg ccagtgcaat agtgctttgt ttcactttat
     4501 tttctccatg tacaaataca ttaaaaaata aaaacaaagc gactataagt ctcggccgtg
     4561 acaactttat gacagctgtt gaaaagatta actttttact gacgaggatg cttcaataac
     4621 ttctttacgt aatcgcgcag cagctccgta tcgtcgtcag gaatgctggc atcgggcttt
     4681 acctcgtaca gcgccccctc tacctgatca atcaaccgct gttggtcatt ttgcgccata
     4741 ttgcgaagca ttgcagtgac gataatctcc aaagcttcta cctgcgcaca cagttctttc
     4801 gactcttctt ctttttgggc aagcttaaat aacaactcag caatgagatt tttcatgtct
     4861 gtatttcctt atccaaagta tggagaagtt aacattatgt ttcacaactt catagtgtct
     4921 ggaagtatta tttttatgac aaaggaaata ctttgcgcaa atattttttg tttgattacc
     4981 agcgaaacgt ttctctt
//
LOCUS       DAPHDW010000218         4974 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.223, whole genome shotgun sequence.
ACCESSION   DAPHDW010000218 DAPHDW010000000
VERSION     DAPHDW010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.223"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..787)
                     /gene="wzm"
                     /locus_tag="RH057_004290"
     CDS             complement(<1..787)
                     /gene="wzm"
                     /locus_tag="RH057_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O8 family O-antigen export ABC transporter
                     permease subunit"
                     /protein_id="HDV3130559.1"
                     /translation="MRDLLTTIYRYRGFIWSSVKRDFQARYQTSMLGALWLVLQPLSM
                     ILVYTLVFSEVMKARMPDNTGSFAYSIYLCSGVLTWGLFTEMLDKGQSVFINNANLIK
                     KLSFPKICLPIIVTLSAVLNFAIIFSLFLIFIIVTGNFPGWLFLSVIPVLLLQILFAG
                     GLGMILGVMNVFFRDVGQLVGVALQFWFWFTPIVYVLNSLPAWAKNLMMYNPMTRIMQ
                     SYQSIFAYHLAPNWYSLWPVLALAIIFCVIGFRMFEVVNKNWPP"
     gene            complement(789..2165)
                     /gene="rfbK2"
                     /locus_tag="RH057_004291"
     CDS             complement(789..2165)
                     /gene="rfbK2"
                     /locus_tag="RH057_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003596869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O9 family phosphomannomutase RfbK2"
                     /protein_id="HDV3130560.1"
                     /translation="MTQLTCFKAYDIRGELGEELNEDIAYRIGRAYGEFLKPGKIVVG
                     GDVRLTSESLKLALARGLMDAGTDVLDIGLSGTEEIYFATFHLGVDGGIEVTASHNPM
                     NYNGMKLVRENAKPISGDTGLRDIQRLAEENQFPPVDPARRGTLSKISVLKEYVDHLM
                     SYVDFSNFTRPLKLVVNSGNGAAGHVIDEVEKRFAAAGVPVTFIKVHHQPDGHFPNGI
                     PNPLLPECRQDTADAVREHQADMGIAFDGDFDRCFLFDDEASFIEGYYIVGLLAEAFL
                     QKQPGAKIIHDPRLTWNTVDIVTRNGGQPVMSKTGHAFIKERMRQEDAIYGGEMSAHH
                     YFRDFAYCDSGMIPWLLVAELLCLKNSSLKSLVADRQKAFPASGEINRKLRNAAEAIA
                     RIRAQYEPAAAHIDTTDGISIEYPEWRFNLRTSNTEPVVRLNVESRADVALMNEKTTE
                     LLHLLSGE"
     gene            complement(2189..3604)
                     /locus_tag="RH057_004292"
     CDS             complement(2189..3604)
                     /locus_tag="RH057_004292"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019725197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HDV3130561.1"
                     /translation="MLLPVIMAGGTGSRLWPMSRELYPKQFLRLFGQNSMLQETITRL
                     SGLEIHEPMVICNEEHRFLVAEQLRQLNKLSNNIILEPVGRNTAPAIALAALQATRYG
                     DDPLMLVLAADHIINNQSAFHDAIRVAEQYADEGHLVTFGIVPNAPETGYGYIQRGVA
                     LTDSAHSAYQVARFVEKPDRERAEAYLASGEYYWNSGMFMFRAKKYLIELAKYRPDIL
                     ETCQAAVNAADNGSDFINIPHDIFCECPDESVDYAVMEKTADAVVVGLDADWSDVGSW
                     SALWEVSPKDEQGNVLSGDAWVHNSENCYINSDEKLVAAIGVENLVIVSTKDAVLVMN
                     RERSQDVKKAVEFLKQNQRSEYKRHREIYRPWGRCDVVVQTPRFNVNRITVKPGGAFS
                     MQMHHHRVEHWVILAGTGQVTVNGKQFLLSENQSTFIPIGAEHCLENPGCIPLEVLEI
                     QSGAYLGEDDIIRIKDQYGRC"
     gene            complement(3882..4166)
                     /locus_tag="RH057_004293"
     CDS             complement(3882..4166)
                     /locus_tag="RH057_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130562.1"
                     /translation="MSRLSHQSQHNAVVVCKELSSPNARCFSGGEYDVSSYLSSYRKC
                     PFLMVAGISKSLDSFFLMAVRACVLPRVNEKVLMVNLAANVEIVGRFVAW"
     gene            complement(4807..>4974)
                     /locus_tag="RH057_004294"
     CDS             complement(4807..>4974)
                     /locus_tag="RH057_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130563.1"
                     /translation="FKYRNREFWCRGYYVDTVGKNTAKIQDYIKHQLEEDKMGEQLSI
                     PNPGSPFTGRK"
BASE COUNT         1226 a         1395 c         1211 g         1142 t
ORIGIN      
        1 tcggtggcca gtttttgttg accacttcaa acatcctgaa accgatgacg cagaaaataa
       61 tggcgagagc caatactggc catagcgaat accagttggg ggccagatga taggcgaaga
      121 tggactgata agattgcatg atccgagtca tcgggttata catcatcaga ttttttgccc
      181 atgcaggtaa tgaattcagt acataaacaa tgggtgtgaa ccaaaaccag aattgcagcg
      241 caacgccaac caattgcccc acatccctga aaaagacgtt catcacacca aggatcatcc
      301 ccagcccacc ggcaaacagg atctgcaaaa gcaggaccgg tatcaccgag agaaagagcc
      361 agccggggaa gttaccggtg acaatgataa aaattagaaa caaactgaaa ataatcgcga
      421 aatttagcac cgccgataac gtcacgatga tcggcagaca gattttcgga aaactgagtt
      481 tcttgatcag attagcattg ttaataaata cgctctgacc tttatccagc atctcagtaa
      541 ataatcccca ggtcagtacc ccggaacaga gataaatact ataggcaaac gacccggtat
      601 tatcgggcat tcttgccttc atcacctcgg aaaaaaccag ggtatagacc agaatcatag
      661 agagtggttg taaaacgagc catagtgcgc ccagcatact agtttgatag cgcgcctgaa
      721 aatcacgttt aacactgctc cagataaatc cccgataacg ataaatcgtc gttagtaaat
      781 ctctcacctt attccccgct taacaggtgt aacagctcgg tcgttttttc attcataagc
      841 gccacatcag ctctggactc aacgttcaga cgtaccaccg gctcggtgtt agacgtgcgc
      901 aggttaaagc gccattcagg gtattcaata ctgatcccgt ccgttgtgtc gatgtgtgca
      961 gccgccggct catactgcgc ccggatgcgg gcgatcgcct cagcagcatt ccttagcttg
     1021 cggttgatct ctcccgacgc agggaacgcc ttctggcggt ccgccaccag cgatttcagc
     1081 gagctgttct tcaaacacag cagctccgcc accagcagcc acgggatcat cccgctatcg
     1141 cagtaggcga aatcgcggaa gtaatggtgc gcactcatct ccccgccgta gatagcgtct
     1201 tcctgacgca tccgctcctt gatgaacgca tgccccgtct tcgacatcac cggctgaccg
     1261 ccgttgcggg tcacgatgtc taccgtgttc cacgtcaagc gcgggtcgtg aatgattttc
     1321 gctcccggct gcttctgcag gaacgcctca gccagcaggc cgacaatgta atacccctcg
     1381 ataaacgaag cttcgtcatc gaacaggaag cagcgatcga agtcgccgtc aaaggcaatc
     1441 cccatgtcgg cctgatgctc gcgcaccgcg tcggcggtat cctggcggca ctccggcagc
     1501 agcggattcg ggataccgtt agggaaatga ccatccggct ggtgatgcac cttgataaag
     1561 gttaccggca ccccagccgc cgcgaagcgt ttctccacct catcaatcac gtgccccgca
     1621 gccccgtttc cggagttcac caccaacttc agtggacggg tgaagttcga gaagtccacg
     1681 tagctcatca gatggtcaac atactccttc agtaccgata tcttgctcag ggtcccgcga
     1741 cgcgccgggt ccactggcgg gaactggttt tcctccgcca ggcgctggat atcccgcagg
     1801 ccggtgtcgc cgctgatggg cttcgcattc tcgcgcacca gcttcatgcc gttgtagttc
     1861 ataggattgt ggctcgctgt cacctcgatg ccaccatcta ccccaaggtg gaaggtggca
     1921 aagtaaatct cttcggtacc gctcaggccg atgtccagca cgtcggtacc ggcgtccatt
     1981 aacccgcggg ccagcgccag cttcagcgac tcgcttgtga ggcgcacatc gccccccacc
     2041 actatcttcc cgggtttcag aaattcgccg taggcgcgac cgatacggta ggcgatgtcc
     2101 tcgttcagtt cctcacccag ttcaccacgg atgtcataag ctttaaaaca agttaactgt
     2161 gtcattctgc gtcttgtccc gaaaataatt agcaacgacc atactggtct ttaatacgaa
     2221 taatgtcgtc ctcgccaagg tacgcccccg actggatctc cagcacttcc agcggaatac
     2281 agccagggtt ttccaggcag tgctcggcgc caatcggaat aaaggtggac tggttctcgg
     2341 acaacaggaa ctgcttaccg ttgacagtca cctgaccggt gccggcgaga ataacccaat
     2401 gctcgacgcg atggtggtgc atctgcatcg agaaggcacc gcctggtttc accgtgatac
     2461 ggttgacgtt gaagcgcggg gtctggacca ccacgtcgca gcggccccag gggcggtaaa
     2521 tctcacggtg gcgcttgtac tcgctgcgct ggttctgctt gaggaactcg accgccttct
     2581 tcacgtcctg ggaacgctcg cgattcatca ccagcacagc gtccttagtg ctgacaatca
     2641 ccagattctc tacgccaatg gccgccacta gcttctcgtc gctgttgatg tagcagtttt
     2701 cgctgttgtg tacccacgcg tcgccgctaa ggacgttgcc ctgctcgtct ttcgggctga
     2761 cctcccatag tgcggaccag gagccaacgt cgctccagtc agcatcgaga ccgaccacca
     2821 ccgcatcagc ggttttctcc atgacggcgt agtccacgga ttcatccggg cactcgcaga
     2881 aaatatcatg cgggatattg atgaaatcgc ttccattatc ggcggcattc accgcagcct
     2941 ggcaggtttc caggatatcc ggacggtatt ttgccagctc gatgaggtat ttcttggcgc
     3001 ggaacataaa catgccgctg ttccagtagt actccccgga ggcgaggtaa gcctcggcgc
     3061 gctcgcggtc cggcttctcc acaaagcggg ccacctggta cgcggaatgg gcactatcgg
     3121 tgagcgccac gccgcgctga atgtaaccgt agccagtttc cggggcattc ggcacgatac
     3181 cgaaggtgac cagatgacct tcatcagcat actgctcggc cacccggata gcgtcgtgga
     3241 aggccgactg gttattgatg atgtggtcgg cggcgagaac cagcatcagc gggtcgtcac
     3301 cgtagcgggt ggcctgaagg gccgccaggg cgatggccgg cgcggtgttg cgcccgactg
     3361 gctcaagtat aatgttattc gacagcttat ttagctggcg cagctgttca gccaccagga
     3421 agcgatgctc ttcgttacag atgaccatcg gttcatggat ttcaagaccc gagagtcggg
     3481 tgatggtttc ctgcagcatg gagttctgcc cgaacaggcg gaggaactgt ttcggataaa
     3541 gctcgcgtga catcggccag agacgactgc cggtaccacc agccataatt acaggaagta
     3601 acataatgaa atccttaaat aattgtttta ttttactatc aatcaatcga ccaacaggtt
     3661 gaagggcctt tttcaggtac gctaccgcca ttggctatcg caaccaaaat gcgtagtgct
     3721 cccgcgctcc aggctaaacg ccaccctctt ttgcttcaga atgtcggcaa ccccttcact
     3781 cagcggtctc aaaaaccatc cttacggaac gggcatcctg aaaacctaaa agcagagata
     3841 tatctcacag catatctctg cttttaatat gtatctgttc ctcaccaggc cacgaaacgc
     3901 cccacaattt ccacatttgc cgccagatta accatcagga ccttctcatt tacgcggggc
     3961 aaaacacatg ctctcaccgc catcaaaaaa aacgaatcaa gtgatttact gatgccggca
     4021 accatcagaa aggggcactt acgatacgag gaaagataag aagagacatc gtattcgccg
     4081 ccagagaagc agcgcgcgtt cgggcttgac agctctttgc acacgacgac ggcgttatgt
     4141 tgcgactggt gggataaacg actcatgcct ctacactgca cattccacaa aaaaatcttc
     4201 gcagattata gcgttgcatg ctagaaaaaa aatataaaag ccgactaatt aatggattac
     4261 tcaaataata tacacatcag acatttacca aactaattaa tgctaattca ttgatataaa
     4321 ggtaataata aagagaatca tgacattttt ttgcctggtt gctaaataat ctgctgataa
     4381 aaaaaacaga tttgtacaaa attacgccat gctcgctgtg agtaaccccg ttttgcgacg
     4441 gtacatgcta accaatgagg tgcctgattt gatgtatcac ctgctggtgc tgttgcaggt
     4501 tcaaatgctg aatttaacaa caggatagtg attctgcgca cgcggcatca gtgagttgcg
     4561 gggtaggcgg atgtgatgtt gctgtcactt ccggcaacaa ctcatccgtt attgcccggt
     4621 gtgcaggttt atcgtgccta caaattttct caacatatgt aagctggata agatacgccg
     4681 gcatcgcatc cgacaaaaat aaatatcctc cggcatagcc ggaggttttt caaatgcgcc
     4741 tataaggctc tcttaccagc cgcgccctaa caggcgcaca cgatctgaca tttgcatcca
     4801 acttcgttac ttacggcccg taaacgggct gcccggattg ggaatcgata actgctctcc
     4861 cattttatcc tcttcaagct ggtgctttat gtaatcctgt atcttcgccg tgttcttacc
     4921 cactgtatcg acgtagtacc ctctgcacca gaactccctg ttcctgtatt tgaa
//
LOCUS       DAPHDW010000219         4912 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.224, whole genome shotgun sequence.
ACCESSION   DAPHDW010000219 DAPHDW010000000
VERSION     DAPHDW010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4912
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.224"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..601
                     /locus_tag="RH057_004295"
     CDS             <1..601
                     /locus_tag="RH057_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HDV3130564.1"
                     /translation="GIGYAVIRRTIFIVYPPILSNGMFNFIVMQTLFYLPFFILGALA
                     FIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETESVITMVLGLWMVN
                     VVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHITSNWLGFLCGLI
                     FVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            complement(609..2090)
                     /gene="clsC"
                     /locus_tag="RH057_004296"
     CDS             complement(609..2090)
                     /gene="clsC"
                     /locus_tag="RH057_004296"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HDV3130565.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEIILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2032..2565)
                     /gene="ymdB"
                     /locus_tag="RH057_004297"
     CDS             complement(2032..2565)
                     /gene="ymdB"
                     /locus_tag="RH057_004297"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HDV3130566.1"
                     /translation="MKTCIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGALPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYGYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            complement(2660..2971)
                     /locus_tag="RH057_004298"
     CDS             complement(2660..2971)
                     /locus_tag="RH057_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3130567.1"
                     /translation="MFRPFLNSLMLGSLLFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSVPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            complement(3092..3424)
                     /gene="csgC"
                     /locus_tag="RH057_004299"
     CDS             complement(3092..3424)
                     /gene="csgC"
                     /locus_tag="RH057_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HDV3130568.1"
                     /translation="MNALLLLAALSSQITFNTTQQGDVYTIIPEVTLTQSCLCRVQIL
                     SLREGSSGQSQTKQEKTLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQSLHLSQQ
                     WPPSSEKS"
     gene            complement(3483..3938)
                     /gene="csgA"
                     /locus_tag="RH057_004300"
     CDS             complement(3483..3938)
                     /gene="csgA"
                     /locus_tag="RH057_004300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin major subunit CsgA"
                     /protein_id="HDV3130569.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGGGNNSGPNSE
                     LNIYQYGGGNSALALQTDARNSDLTITQHGGGNGADVGQGSDDSSIDLTQRGFGNSAT
                     LDQWNGKNSEMTVKQFGGGNGAAVDQTASNSSVNVTQVGFGNNATAHQY"
     gene            complement(3979..4434)
                     /gene="csgB"
                     /locus_tag="RH057_004301"
     CDS             complement(3979..4434)
                     /gene="csgB"
                     /locus_tag="RH057_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HDV3130570.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAIVVQRQSQMAIRVTQR"
BASE COUNT         1254 a         1209 c         1075 g         1374 t
ORIGIN      
        1 cggcatcggt tatgcggtaa taagaagaac gatttttatt gtgtatccgc ccattctgag
       61 taatggcatg ttcaatttta ttgtcatgca aacgctgttt tatttgccgt tctttatcct
      121 cggcgcactg gctttcattt tccctcatct taaagccttg tttaccacgc cgtctcgtgg
      181 ctgtaccctt gcagcagcat tggcgtttgt cgcttattta ctcaaccagc gctatggcag
      241 tggcgatgcc tggatgtacg aaaccgagtc ggtgatcacc atggtcctcg gtctgtggat
      301 ggtgaatgtg gtcttctcct ttggccaccg tttgcttaac ttccagtcag cgcgggtgac
      361 ttattttgtt aacgcatcgc tgtttatcta tctggttcac cacccgttaa cgctgttttt
      421 cggcgcatac attacaccgc acatcacctc caactggctt ggttttctct gtggcctgat
      481 atttgtagta gggattgcga taattctgta tgaaattcat ttgcgcatcc cgttactgaa
      541 gtttttgttc tctggtaaac cggttgttaa gcgtgagaac gataaagcac cagcccgtta
      601 agccacattt acaataacca ttccacgggc aatatcgacg ccagtctgac cataacccgc
      661 ttccagaaac tggtggcggg ttcttttttg agaataatct ctttctttgc atgacgatca
      721 acccagttga tccgtcccca cctgtccaga cggagctgcc aggccgcatc atactggctc
      781 tgaataaagc gtttatcaat taactgtgcc agcgtttcgc tctctatcac gaagcccatt
      841 tcagtattga gcaatgttga acgcggatcg aaattgaaag agccgataaa caccgtttta
      901 ccatcgatgc taaaggtttt agcatgcagg ctggctccgg aattaccggt tatgccgcga
      961 tcgtgtaacg tactactttg ttcacgcgtc ggcttgagtt catataattc cacgccatag
     1021 cggagcaatt ttttgcgcca gcgcgcgtat ccggcatgga cgacagcaac atcgttagcg
     1081 gcaagagaat tggttaggat cgcaatcttt accccttttc tcaccatccg taagagttgc
     1141 gccacacctg cgcgtgtcgg tacaaaatag gaagagataa tatcgatgcg ttcactgggt
     1201 gagcccatga tatcgaacag gcgctgcggt aacagtgaat gccgttttgc cttgccctcc
     1261 cctttcgccg gatcatcact taataaacgt gttttcgccc agataagcgg caacgttcca
     1321 tcaaccagat gatttataaa cggactggat tccattttgc gtaagtaacg atgcgtcata
     1381 gcatcgttat gccaggaggc gggtaactcg atgcgatccg ccatttcacc ttccgggaca
     1441 tccagcactt gctgtaaggg tgaaaccgat ttgcaatacc agtagcgggc gaaatcatcg
     1501 gcaacgtcct ctaccacggg ccctattgcc ataacatcta aatccgaaaa aagtggctct
     1561 tcccctgccc caaaataggc atcaccaata tttcgccccc ctaccagcgt caccacgcca
     1621 tcgacagtga aacttttatt gtgcatacgg cgattgagac gggaaaagtc ggtgatataa
     1681 ccaagcggac gcagcaagcg aaacgagaaa ggattaaaaa gccggacttc aatgcgggga
     1741 tggctgtcaa gcaagcgtaa aatgtcgtca agtccgggcg tattgttgtc gtccagcaac
     1801 aaacggacgc gaacgccacg ctttgctgcg gctaacaggg cggaaaacag taaccgaccc
     1861 gacatatcgt cctgccagat gtaatactga acatccagcg tatgctctgc catttcggcc
     1921 agacgatacc gggcggcaaa cgcatccaga cttttctcca gagggaaaag accacactga
     1981 ccgggatgtt gcgaacacag tggcagcacc gcgctcgcca gccggggcaa atcattcatc
     2041 tccttgttgg gtaaggagtc tttcgtagag gtgggcgttt tcttcatcat aacagacaaa
     2101 gtatacctgt tcgggtaaag cgtgacgggt aataaattct gaaacggttt taactgcgat
     2161 ttcagccgct gccgcacgag ggtaaccata aaccccagta ctgattgcag gaaaagccac
     2221 tgacgtatag ctgtttgccg ccaccagtcg taggctattg agataggcat cctgcaaaag
     2281 ctggtcttcg ttttgttcac caccacgcca gactggcccg acggtgtgca ctacggcttt
     2341 agcgggaaga gcgcctgcaa gcgtaataac ggcatgcccc gtagggcaat cgccctgctg
     2401 ttgcctgact tttaaacagg catccagcag ggccggaccc gctgcgcgat gaatggcccc
     2461 atcgacgccg ccgcctccca ttaatgacgg attagccgca ttcacaatca catcaacggc
     2521 cagtttggta atatcaccct gcacaacatg aatacacgtt ttcataacgc ctccttacac
     2581 cttcttgttt cttaagtgta cagcaggctt tttggttaaa tcgcattttt ctgctcagca
     2641 acgtgcgttg ttcagaaact tagttatatt cgatgttcat tacagccagc tttttctgtt
     2701 tatcaagata ctgcactttt actgaggcta tttgcgcgtt tttaacatta ccgctcatta
     2761 acgctttgct ggaataggtt ttgcccggaa cagaaccatt ctgtgggcag ttcatttcta
     2821 cgggtgatga ctgggtgctg acgtcacatg ccggttccac aatttggcca taaaaatgga
     2881 tcaccccccc ttgcgcggtg cttcctgcga tggcaataaa aggaaaaagc aaactgccga
     2941 gcataagaga gtttaaaaaa ggtctgaaca tttattctat ttcctcaatg attagtcatc
     3001 cttgagggtt gtgttatcca tactttcagg ttggctgatt cgcagcagac cattctctcc
     3061 agattcatct tatgctcgat atttcaacaa attaagactt ttctgaagag ggcggccatt
     3121 gttgtgataa atgaagtgac tgtccatcag aaacagtaac aactattttc acccgatctt
     3181 ccggggaaat atttaaactc aacttcgtca aagcaatagg ttgattagca ggcaatgaga
     3241 gggtcttttc ttgcttcgtc tgactttgcc ctgaactgcc ttcgcgcagg gacaatattt
     3301 gtactctgca cagacaagat tgagtaagag tgacttcagg aataatggta tacacatccc
     3361 cttgctgggt cgtattaaag gttatctggc tggaaagtgc cgcaaggagt aataacgcat
     3421 tcatattctt ctcccgaaaa aaaacagggc ttgcgccctg tttctgtaat acaaatgatg
     3481 tattagtact gatgagcggt cgcgttgtta ccaaagccaa cctgagtcac gttgacggag
     3541 gagttagatg cagtctggtc aactgcagca ccgttgccac caccgaactg tttaacagtc
     3601 atttcagaat ttttgccgtt ccactgatca agagtggcgc tgttaccgaa gccacgttgg
     3661 gtcagatcga ttgagctgtc atctgagccc tgacctacat ctgcaccatt accaccgcca
     3721 tgctgggtaa tagtcaagtc agagttacgg gcatcagttt gcagagcaag tgcagagtta
     3781 ccgccaccgt actggtaaat gttcagctct gaatttgggc cgctattatt accgccacca
     3841 ccgtggttac cgccgccgcc gtactgagga acaacacctg ccagagcgct accggagaat
     3901 acgattgctg caattgctgc tacttttaaa agtttcatgt aaaaccccca tcggattgat
     3961 ttaaaagtcg aatggaaatt aacgttgtgt cacgcgaata gccatttgcg actgtctctg
     4021 cactacaatt gccgtttttt gagtaccata ctgtgtaata tttgctttat taccagaacc
     4081 tttctggata atcatcgcag tattaccata agcaccttgc gaaatactgg catcgttggc
     4141 actgcccgcc tgatcaatat atgcaaggtt ataatctcct gtctggtcaa tctttgcccg
     4201 gttgctacta ccttcttgcg caacaaccgc caaaagtttt gagcctccct gccgtaactg
     4261 agcactatta ttcgtcccag cttgaccaat tatggctgcc tgattaaatg aagacttact
     4321 caattcattt accgcgaagt tatattctga attagctaaa tcataacctg ctgcggctgc
     4381 aatcccaggc gcacccagta ttgttaacat cataaataac aatttgtttt tcatgttgtc
     4441 accctggacc tggtcgtaca tttaagaaat taaatcattt caacttggtt gttaacgcaa
     4501 cctgtatttt gttaacgctg cgttacgatg gaaagtatgt ctgcggaaat atttttaata
     4561 actcacccgc gcgttgtatt tttctttttt tgttcatacc aaagtattaa aacccacgta
     4621 aatacgctgt tatctacgca aaaaatattt tgttttcttt taaacctccg ttttccgcta
     4681 tcaaaaatca ccagacagtc atttttcttg cccgtcgctg attgctgcga tatgtcttgc
     4741 gcacaagccg tgacaaacct cgtctttttg cagtaaaaaa ttgtccacgg aggtgcggag
     4801 aaaaaacaag aacgttttac atgacgaaag gactacaccg aaatattttt tatatgtatt
     4861 attagtaagt tatcaccatt tgtatgattt tttaaaattg tgcaataaaa ac
//
LOCUS       DAPHDW010000220         4901 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.225, whole genome shotgun sequence.
ACCESSION   DAPHDW010000220 DAPHDW010000000
VERSION     DAPHDW010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4901
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.225"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..395
                     /locus_tag="RH057_004302"
     CDS             <1..395
                     /locus_tag="RH057_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDV3130571.1"
                     /translation="FGHGSAQHVRAGFRLGSHNDMTFGEGTSSRAPLRDSAKHSVSEL
                     PVNWWVQPSVIRTFSSRGDMRVGTSTAGSGMTFSPSQNGTSLDLQAGLEARVRENITL
                     GVQAGYAHSVSGSSAEGYNGQATLNVTF"
     gene            516..3032
                     /locus_tag="RH057_004303"
     CDS             516..3032
                     /locus_tag="RH057_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130572.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALISTASALAGLILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAGGLLAVPLQIKNIQQNGITPEDISKEINGYYCCFYTAFFLMACSACAPLIALQYD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKKLTCAISQTLEEQPVLNSKSWLTSLQ
                     NDYSLPDSLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPDPEWYCSSLCRETETLCQEIYERPYNSFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNNGPMQLEVPRDQYAGAVETMRNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLSVVQGMLKHIDFSTASPT
                     VRLALQKGTGAGNISALNKVMKGTLVTSLALVAVTTGPDMIKMLRGRISGTQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNENLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGNMVNRLEEALPPELPA"
     gene            3108..3563
                     /locus_tag="RH057_004304"
     CDS             3108..3563
                     /locus_tag="RH057_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130573.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNDEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFNPQDYHPLLITVSGKNVN"
     gene            3642..3875
                     /locus_tag="RH057_004305"
     CDS             3642..3875
                     /locus_tag="RH057_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001119724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HDV3130574.1"
                     /translation="MPGYTEYVLAEGSFSYGQAVAVITAYRNVFIQDDPGMHFRRVIR
                     NAEGQRRWRCRNSEPDAGKVLNTRLASDGLLRQ"
     gene            3975..4793
                     /locus_tag="RH057_004306"
     CDS             3975..4793
                     /locus_tag="RH057_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HDV3130575.1"
                     /translation="MRLASRFGYANQIRRDRPLTHEELMHYVPGIFGEDKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVDRVIEGAYEVVGVFD
                     RIEEKRDAMQSLILPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     AYQTIQENMLKGGISGRSAKGKRIHTRAIHSIDTDIKLNRALWVMAETLLESLR"
BASE COUNT         1185 a         1271 c         1304 g         1141 t
ORIGIN      
        1 agttcgggca tggcagtgca caacatgtgc gtgccggttt ccgtctgggc agccacaacg
       61 atatgacctt tggcgaaggc acctcatccc gtgcccccct gcgtgacagt gcaaaacaca
      121 gtgtgagtga attaccggtg aactggtggg tacagccttc tgttatccgc accttcagct
      181 cccggggaga tatgcgtgtg gggacttcca ctgcaggcag cgggatgacg ttctctccct
      241 cacagaatgg cacatcactg gacctgcagg ccggactgga agcccgtgtc cgggaaaata
      301 tcaccctggg cgttcaggcc ggttatgccc acagcgtcag cggcagcagc gctgaagggt
      361 ataacggtca ggccacactg aatgtgacct tctgacagaa ccatcgcctc tctgtggtcc
      421 cggtcatcat gaccgggacc cggaccggcg caacggatct tcaacgccac attcgctggc
      481 attaacaata acatgatatt catcacggag tgactatgtt acagatagtc ggcgcgctga
      541 tcctgctgat cgcaggattt gccattcttc gccttttgtt cagagcatta atcagcacgg
      601 cttctgcgct ggcagggctc atattgctgt gtctgttcgg cccggcctta ctggctggct
      661 atatcaccga acgcataacc cggttgttcc atattcgctg gctggcaggc gtatttctga
      721 cgattgccgg aatgatcatc agcttcatgt ggggacttga tggtaaacat atcgcgctgg
      781 aggctcacac ctttgactct gtgaaattta ttctgaccac tgctctcgcc ggtggtctgc
      841 tggctgttcc cctgcagatc aaaaacattc agcagaacgg gatcacacca gaagatatca
      901 gcaaggaaat taacgggtat tactgctgtt tttatactgc ctttttcctt atggcgtgtt
      961 ctgcatgcgc accattgatc gcgttacagt acgatatttc accgtcactg atgtggtggg
     1021 gcgggttgtt gtactggctg gctgcattag tgacgctgct atgggcggcc agccagatcc
     1081 aggcgctgaa aaaactgacc tgtgccatca gccagacact ggaagaacaa ccggtgctca
     1141 acagtaaatc gtggctgacc agtttgcaaa acgattacag ccttcctgac tcactgacgg
     1201 agcgcatctg gctgacgctc atttctcaac ggatttcccg gggagagctg agggaatttg
     1261 aactggcaga cggaaactgg ttactgaaca atgcctggta tgaaagaaac atggcagggt
     1321 ttaacgaaca gttgaaagag aacctgtcat tcacacctga tgaactgaaa acgctcttcc
     1381 ggaaccgcct gaatttatca ccggaagcga atgacgattt tctcgatcgt tgcctggacg
     1441 gcggtgactg gtatcccttt tcagaaggtc gccgttttgt atcattccat cacgtggatg
     1501 agcttcgtat ctgtgcctcc tgcgggctga cagaagtaca tcatgccccg gaaaatcata
     1561 agccggatcc ggaatggtac tgctcctctc tttgtcgcga aacagaaaca ctgtgtcagg
     1621 aaatttatga acgcccttac aacagcttta tttccgatgc aacggcgaat ggtctgattc
     1681 tcatgaaact gccggaaacc tggagtacaa atgagaaaat gtttgcttcc ggagggcagg
     1741 gacatgggtt tgccgctgaa cggggaaacc atattgtcga cagagtccgt ctgaaaaacg
     1801 cacggatcct cggtgataat aatgccagaa atggagcaga cagactggtc agcggaacag
     1861 aaatccagac gaaatattgt tcaactgcag cccgtagcgt cggtgcggca ttcgacggac
     1921 aaaacggaca gtatcgttac atgggaaata atggccccat gcaactggaa gtcccccgtg
     1981 atcagtatgc cggcgctgtt gaaaccatga ggaataagat ccgcgaaggt aaagtacccg
     2041 gtgtaaccga tcccgcagaa gcatcccggc tgattcgtcg gggacatctg acttatactc
     2101 aggcccgtaa tatcacccgg ttcgggacca tcgaatcggt cacttatgat attgccgagg
     2161 gttcggttgt cagtctggcg gccggaggga tcagttttgc cctgacggca tcggtcttct
     2221 ggctcagcac cggcgatcgc gatgctgccc tgcagacagc tgctgtccag gcaggaaaaa
     2281 ccttcacccg cacactggct gtctacgtca caacccagca actccaccgg ctcagtgttg
     2341 ttcagggtat gctgaagcac attgattttt cgactgccag cccgactgtc cgactggctc
     2401 ttcagaaggg gaccggtgca ggaaatatca gtgccctgaa caaagtgatg aagggtacgc
     2461 tggtgacatc tctggcactg gtagctgtca caaccggccc tgacatgatc aaaatgttgc
     2521 ggggacggat ctccggtacg cagttcatca ggaatcttgc cgtggcatct tccggtgtgg
     2581 caggtggagc tgtcgggtca gtggcgggcg ggatactgtt cagtccactg ggaccatttg
     2641 gtgcactgac agggcgtgtg gttggcggtg ttctgggggg aatgattgcc tccgctgtat
     2701 caggaaaaat tgccggagcg ctggttgaag aagatcgcgt caaaattctg gcaatgattc
     2761 aggagcaggt gacatggctt gccggcagtt tcctgctgac cggacatgag attgaaaatc
     2821 tgaacgagaa tctggcccgt gttatcgatc agaatgctct ggagatcatt ttcgccgccg
     2881 gtatacaaca acgggccgcg accaatatgt taatcaaacc actggtggtc agtatcatca
     2941 ggcaacgccc cgtcatggaa tatgatgcat cccatctcgg caatatggtt aaccgactgg
     3001 aagaagcatt acccccggag ttaccggcat aacggctatt ctgcgccttt tacgctttat
     3061 cacctgattt gtcagtgtca tcgtcatgac tcagagaggt aaataccatg attcacctgt
     3121 tcaaaacctg catgattacc gccttcattc tggggttaac gtggtctgcc ccactccggg
     3181 cacaggatca acgttacatc agtatacgca atacagatac gatatggctc ccgggaaata
     3241 tttgtgctta ccagttccgg ctggataatg gcggaaacga tgaaggattt ggccccctca
     3301 ccatcactct gcaactcaaa gacaaatatg gtcagacgct ggtgaccaga aaaatggaaa
     3361 cggaagcttt tggtgacagt aatgccacgc gaaccacaga cgcatttctg gaaacggagt
     3421 gcgtggaaaa tgtcgccaca accgaaatca ttaaagcaac tgaagaaagt aacggccatc
     3481 gtgtcagtct gccgttatcg gttttcaatc cccaggacta ccatccactg ctgattaccg
     3541 tttccggaaa aaacgttaac tgatccctga agtcagtttc atgcttcagc ctctgtcaca
     3601 acgcgtggca gaggcttttt atttatcagg agaaaacaga tatgccgggt tacacagagt
     3661 atgtcctggc tgaaggatct ttctcatacg ggcaggcggt tgctgtcata accgcttacc
     3721 gcaatgtgtt tatccaggat gacccgggaa tgcatttccg tcgggttatc cgcaacgcgg
     3781 aaggtcagcg acgctggcgc tgccggaatt ctgaacccga tgccggaaag gtgcttaata
     3841 cgcggctcgc cagtgacggt cttctcagac aataaacgtc attcatttca cccatcaggc
     3901 cgcgtcttct ccgggagacg tggctttttc atttattaca ccagtaaatc ttaaccaccg
     3961 ataaggagca aattatgcga ctagccagtc gttttggtta tgcgaaccag atacgccgtg
     4021 accgtccgct gacacacgaa gaactgatgc actatgtgcc ggggattttc ggggaagata
     4081 aacacacgtc ccgaagccag aactatacgt acatccccac catcaccgta ctggaaagcc
     4141 tgcagcggga aggctttcag ccattcttcg cctgccagac ccgtgtgcgc gacccgggcc
     4201 gccggggata cacaaaacac atgctgcgtc tgcggcgggc cggagagata aacggagaac
     4261 atgtccctga aattattctg ctcaactctc atgacggtac ctccagctac cagatgctgc
     4321 cgggttactt caggttcgtc tgccagaacg gatgcgtctg tggtcagtct ctgggggaag
     4381 tgcgtgttcc gcaccgggga aatgtggtgg acagagtcat tgaaggggct tacgaggtgg
     4441 tgggggtttt tgaccggatt gaggaaaagc gtgatgccat gcagtcgctg attctgccgc
     4501 caccggcacg ccaggcgctg gcacaggcgg cactgactta ccgttatggt gacgaacatc
     4561 agcccgtcac caccgccgac attctgacgc cacgacgccg ggaggattac ggtaaggacc
     4621 tgtggagtgc ttatcagacc attcaggaga atatgctgaa aggcggaatt tcaggtcgca
     4681 gcgctaaagg aaaacgtatc catacccgtg ccattcacag cattgacact gacattaagc
     4741 tcaaccgcgc attgtgggtg atggcggaaa ccctgctgga gagcctgcgc tgattcagcc
     4801 attaccctga aaaccacgca tttatttgtc ctttaaggag tcatttcatg aaaaccattt
     4861 cacataattc caccacatct tctgtttccg taaccgctgc a
//
LOCUS       DAPHDW010000221         4888 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.226, whole genome shotgun sequence.
ACCESSION   DAPHDW010000221 DAPHDW010000000
VERSION     DAPHDW010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4888
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.226"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(69..830)
                     /gene="kduD"
                     /locus_tag="RH057_004307"
     CDS             complement(69..830)
                     /gene="kduD"
                     /locus_tag="RH057_004307"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HDV3130576.1"
                     /translation="MILSAFSLEGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPALLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(860..1696)
                     /gene="kduI"
                     /locus_tag="RH057_004308"
     CDS             complement(860..1696)
                     /gene="kduI"
                     /locus_tag="RH057_004308"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HDV3130577.1"
                     /translation="MDVRQSIHSAHAKTLDTQGLRNEFLVEEVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFDDMDHVAVKD
                     LR"
     gene            complement(1983..3164)
                     /locus_tag="RH057_004309"
     CDS             complement(1983..3164)
                     /locus_tag="RH057_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HDV3130578.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTSVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGAQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMTQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            3419..4648
                     /locus_tag="RH057_004310"
     CDS             3419..4648
                     /locus_tag="RH057_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HDV3130579.1"
                     /translation="MSNIWSKEETLWSFALYGTAVGAGTLFLPIQLGSAGAVVLFITA
                     LVAWPLTYWPHKALCQFILSSKTSAGEGITGAVTHYYGKKIGNLITTLYFIAFFVVVL
                     IYAVAITNSLTEQLAKHMVIDLRIRMLVSLGVVLILNLIFLMGRHATIRVMGFLVFPL
                     IAYFLFLSIYLVGSWQPDLLTTQVEFNQNTLHQIWISIPVMVFAFSHTPIISTFAIDR
                     REKYGEHAMDKCKKIMKVAYLIICISVLFFVFSCLLSIPPSYIEAAKEEGVTILSALS
                     MLPNAPAWLSISGIIVAVVAMSKSFLGTYFGVIEGATEVVKTTLQQVGVKKSRAFNRA
                     LSIMLVSLITFIVCCINPNAISMIYAISGPLIAMILFIMPTLSTHLIPALKPWRSIGN
                     LITLIVGILCVSVMFFS"
BASE COUNT         1278 a         1242 c         1066 g         1302 t
ORIGIN      
        1 gattatgatg atctacagat ataaaaaacc ctgccatgcg gcagggtcat aaaagtaaga
       61 agaatgaatt aacgcgccag ccaaccgcca tccacggcaa tggtataacc attcacataa
      121 tctgaagcgc tggaggcaag gaacactacc ggccccatca ggtcactcgg cagtccccaa
      181 cggccagctg gaatgcggtc gaggatttcc gcgctacgtt gttcatctgc ccgtagttgt
      241 tgagtattgt tggtcgccat gtaacccggg gctatcgcat taacattaat gttgtgttta
      301 gcccattcgt tcgccatcaa tcgcgtcaca cccatcacgc cgctttttga tgcggtataa
      361 gaaggcacac ggatcccgcc ctggaaggag agcattgacg cgatattgat aatcttgccg
      421 ccattgcctt gcgcgataaa gtgtttcgcc gctgcctgag acatgaagaa tacgctcttg
      481 atattcaggt tcatgacatc gtcccagtct ttttcgctga actcgagagc gtcttcgcgg
      541 cgaatcaatc cggcgttatt caccaggata tcaatatgac caaactccgc taccgcgcga
      601 tccagcagtg ctggaatacc atcaatcttt cgcagatcgg cggtcaggct taaaaaacga
      661 cgccccagcg ccgtgacctg ctcgatggtt tcagtcggtt caacgatgtt aatgccaaca
      721 atgtcacagc ccgcttgcgc cagccccaac gccatcccct ggcccagccc agtatcacaa
      781 ccagtgacga ccgcaacttt accttcgaga gaaaatgcac ttaaaatcat aacaatacct
      841 tattcgttta tgcccacaac tagcgcaaat ctttaacggc cacatggtcc atatcatcaa
      901 agacctggtt ttcaccgacc atgccccaga taaaggtata agctttggtt ccgacgccgg
      961 aatgaatcga ccagctcggg gagatcaccg cctgctcgtt atgcatcaca atgtgacgcg
     1021 tttcttgcgg ctgccccatc atgtggaaaa cgcaggcgtc atcatccata ttgaaataga
     1081 aataaacttc catccggcgc tcgtgagtgt gacacggcat ggtgttccac aagttacctg
     1141 gagccagctc cgtcagcccc atactcaatt ggcaggtttc cagcacatcc gggacaaaat
     1201 atttgttaat tgtgcgacgg ttactggtga ggttatcgcc taacgtgact ggagatactt
     1261 cgtccggtgt gacttttttg gtgggatacg tcgtatgcgc gggtgcgcaa ttgtaataaa
     1321 actttgccgg agtggcggta tcaatactgg caaagacaac ttcttttgca cctttaccca
     1381 catacagggc gtcgcggtga ccgatttcat agcattggcc atcgacagta atcgtaccgg
     1441 caccgccaat attgataaca cctaactcgc gacgttcaag gaaatagctt acgccgagtt
     1501 gtttaccaac ttccccgcca acggaaaccg ttttagttat cggcataatg ccgccaacaa
     1561 taattcggtc aatgtggctg taaaccatgg tgtactcatc ggcgacaaat acctcttcaa
     1621 ccaaaaattc attgcgcagc ccttgggtat ccagcgtttt tgcgtgcgca ctgtggatgc
     1681 tctgtcttac gtccacatca acctccgata atcagtgaag cgtgaaaata gaaaacgata
     1741 gtgaaacact gtttcgtttt ctctataaag ggatgatatg cccatgggct agggttttca
     1801 attaaaaata aaacaatgtt tcatttttaa gttaaggatt aaaaaaagtg cagtgtcgat
     1861 cacgaatatg tcatgagaat ggaaaaatga gggagcccag aaaatgtatg tcgacttgcc
     1921 agccatcatt attcaacaga ttaatgacgg cagatattta tcggtaagga ggaaatgcag
     1981 agctactcgt cacgttcaat ggtcaatgcc acgccctggc ccccgccgat acaaagcgtt
     2041 gccagtcctt tgcgggcatt acgtttcacc atttcatgaa ccagagaaac caggattcgg
     2101 caaccggaag cgcctatcgg gtgaccaagt gcgatcgcac caccattgac attgacccga
     2161 cgctcatccc actcaagcat cttgccaacc gaaagcgcct gtgcagcaaa cgcttcatta
     2221 gcctcgataa gatcgacatc agccaactgc cagcctacac gctccaggca acggcgggtc
     2281 gcatacaccg gcgcaattcc catcaatgcc ggatctacac caacgctggc aaatgcgcga
     2341 atgcgggcca gcacgggtaa attcaacgct cgtgctttgg cttcgctcat catcattacc
     2401 gcagctgcgc catcgtttat ggatgatgca ttacccgctg tcacagaacc gagactatca
     2461 aatgaaggat ttaaacgggc taaaccttct gcactggcgt cagtgcgtgg ctgttcatcg
     2521 gtatcaacaa ccaacgtctg accgttactt tgggtcatta ccgggacgat ctcatcttta
     2581 aatcgtccgg cgtcaatcgc cgctcgcgct ttttgttgcg agctaagtgc gtaagcatcc
     2641 tgcaattgac ggctgatgcc atattcgcga gccagatttt cggcggtgac accaatatga
     2701 taatcattga aggcatccca taacccatca tgcacaagac tgtcaaccag ctggctattg
     2761 ccaagctgtg caccggtgcg gctatcagtc agaacatgtg gtgcgcggct catgttttcc
     2821 tggccaccgg cgatgacaat atcagcctcg ccacactgta tcgcctgagt agccagatgc
     2881 agtgctttaa gtccggaacc gcagacgtca ttaatcgtga ttgcagaaac gctattaggc
     2941 agaccacctt taatagccga ttgccttgcc ggattctgcc ctgcccctgc agtcaacacc
     3001 tgaccaagaa ttacttcatc caccgcatat gcaggaacgc tggtacgttc tattaacgct
     3061 ttcacgacca gactaccaag ttccacggcg gaatgacccg ctaacgcacc gcgaaagcag
     3121 ccgataggtg tccgtaacgc cccgacaatc acaacgtctt tcatcacaac ctcgcaccaa
     3181 ttaacagtgc aatagtaaat gattgttaat tacagttatc ttaattgttt aaaaaaagtg
     3241 atttttatca caaaggaaat atgcctgagc agtagtcaga gtcataactg gcacgtaagg
     3301 tttgcaacca ctaacccacc aatagagggg tagatagggc gttaatctcc catacttaac
     3361 ctggtttatg gtaaattgcc ctccattttg tttaatttgt agatgatacg ttcagataat
     3421 gtctaatatt tggtctaaag aagaaactct gtggagtttc gcgctctacg gcacagccgt
     3481 tggtgcaggc acgctcttcc ttcctattca gttaggttcg gcaggggctg tggtcctgtt
     3541 tattactgct ctggtcgcct ggcctttaac atattggcca cataaagcct tatgccagtt
     3601 catcctctca tcgaaaacat cagcaggtga agggataacg ggcgcagtaa cacactacta
     3661 tggcaagaag attggtaatc tgattaccac gctgtacttc atcgcctttt ttgtcgtcgt
     3721 gttgatatat gcagtggcaa ttaccaactc acttacggaa cagctggcaa agcatatggt
     3781 tattgatctt cgcatccgta tgttggtgag tctgggtgtt gtattaatcc tgaatctcat
     3841 ttttctgatg ggacgccatg caaccattcg ggtaatgggt tttttggtat tcccattgat
     3901 tgcctatttc ttatttcttt ccatttacct tgtcggtagt tggcaacctg atctattaac
     3961 gacccaggta gagttcaatc agaataccct tcaccagata tggatatcga ttcccgtgat
     4021 ggttttcgcc tttagccata cgcccattat ttctacgttt gccatagaca gacgtgaaaa
     4081 atatggcgaa cacgctatgg ataaatgcaa aaaaattatg aaagtcgctt atctcatcat
     4141 ctgcataagt gtactgttct ttgtctttag ctgcctgctt tctattccac cttcgtatat
     4201 tgaagcggct aaagaagaag gggtcaccat tttatcggcg ctttctatgc tgccgaacgc
     4261 cccagcatgg ctgtcaattt ccgggattat tgtcgcagta gttgcgatgt cgaaatcatt
     4321 cctgggtacg tactttggcg ttattgaagg tgccacggag gtcgtcaaaa caacactaca
     4381 gcaggttggt gtaaagaaaa gtcgtgcatt taaccgcgca ctatcaatta tgttggtatc
     4441 gctgatcacc ttcattgttt gttgcattaa cccgaacgcg atttcgatga tttacgcgat
     4501 cagcggcccg ctcattgcca tgatactttt catcatgcct acgttgtcaa cgcatctgat
     4561 cccggcgctt aaaccctggc gttccatcgg aaatctgatt acgctgatcg tgggtatcct
     4621 gtgcgtatcg gtaatgttct ttagctgata tccattgtga atggttcaaa attgctacaa
     4681 ttttgaacca ttccagtttt cataatgttc attcgttatt attccgcact atcacttccc
     4741 tcatcatatc ctagtattat cagttagtta aacacacaaa gagatctgaa catcttaaat
     4801 aagactattt aagatgcata acttagattc gcaagatatc ttccggtcat tcaaaaacaa
     4861 tttgcgatat agatattctc ataccatg
//
LOCUS       DAPHDW010000222         4794 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.227, whole genome shotgun sequence.
ACCESSION   DAPHDW010000222 DAPHDW010000000
VERSION     DAPHDW010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4794
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.227"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            111..1085
                     /gene="hemB"
                     /locus_tag="RH057_004311"
     CDS             111..1085
                     /gene="hemB"
                     /locus_tag="RH057_004311"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HDV3130580.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            complement(1191..2042)
                     /gene="tauD"
                     /locus_tag="RH057_004312"
     CDS             complement(1191..2042)
                     /gene="tauD"
                     /locus_tag="RH057_004312"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HDV3130581.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQTITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSGLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(2039..2866)
                     /gene="tauC"
                     /locus_tag="RH057_004313"
     CDS             complement(2039..2866)
                     /gene="tauC"
                     /locus_tag="RH057_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HDV3130582.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            complement(2863..3630)
                     /gene="tauB"
                     /locus_tag="RH057_004314"
     CDS             complement(2863..3630)
                     /gene="tauB"
                     /locus_tag="RH057_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HDV3130583.1"
                     /translation="MLQISHLYADYGGKPVLEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSGGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLPLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            complement(3643..4605)
                     /gene="tauA"
                     /locus_tag="RH057_004315"
     CDS             complement(3643..4605)
                     /gene="tauA"
                     /locus_tag="RH057_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HDV3130584.1"
                     /translation="MAISSRNTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNT
                     FAKESGATVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKL
                     GNSEALVVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGQVEIVNLQPPA
                     IIAAWQRGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHP
                     EVVKAFAKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQ
                     QQTVELTGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
BASE COUNT         1100 a         1387 c         1232 g         1075 t
ORIGIN      
        1 tggtctctct ttcattctct tcttgtcaac atcacgacaa ctttcgtaaa acactcctgc
       61 cccgcttcag gtatactgtg caccttaatt ccacaaacat caggcagacc atgacagact
      121 taatccaacg ccctcgtcgc ctgcgcaaat ctcctgcgct gcgcgctatg tttgaagaga
      181 caacacttag ccttaacgac ctggtgttgc cgatctttgt tgaagaagaa attgacgact
      241 acaaagccgt tgaagccatg ccaggcgtga tgcgcattcc agagaaacat ctggcacgcg
      301 aaattgaacg catcgccaac gccggtatcc gttccgtaat gacttttggc atctctcacc
      361 ataccgatga aaccggcagc gacgcctggc gggaagatgg actggtggcg cgtatgtcgc
      421 gcatctgcaa gcagactgtg ccagaaatga tcgtcatgtc agacacttgt ttctgtgaat
      481 acacttctca cggtcactgc ggtgtgctgt gcgagcatgg cgtcgacaac gacgcgactc
      541 tggaaaattt aggcaagcaa gccgtggttg cagctgctgc aggcgcagac ttcatcgccc
      601 cttccgccgc gatggacggc caggtacagg cgattcgtca ggcgctggac gctgcaggct
      661 ttaaagatac ggcgattatg tcgtattcga ccaagttcgc ctcttccttt tatggtccgt
      721 tccgtgaagc tgccggaagc gcattaaaag gcgaccgcaa aagctatcag atgaacccaa
      781 tgaaccgtcg cgaggcgatt cgtgaatcac tgctggatga agcccagggc gcagactgcc
      841 tgatggttaa acctgctgga gcatacctcg acatcgtgcg tgagctgcgt gaacgtactg
      901 aattgccgat tggcgcgtat caggtgagcg gtgagtacgc gatgattaag ttcgccgcgc
      961 tggcgggtgc tatagatgaa gagaaagtcg tgctcgaaag cttaggttca attaagcgtg
     1021 cgggtgcgga tctgattttc agctactttg cgctggattt ggctgagaag aagattctgc
     1081 gttaattttt ttcctctcag aaagggggag ttaacctgtt gaaagtacgg tgaacgcccc
     1141 ctcaccctaa ccctctcccc aagggggcga gggaaccgtc cgcactcgta tcaccccgcc
     1201 cgataaaacg gtttatcccc aagaatcgtc gcccgatgca taatccgccg ctgtggcagg
     1261 taatcggcat tggcatagtg ctgagtcacg cggttatccc aaatcgcaat atcattcggt
     1321 tgccagcgcc aacgcacctg aaactccggt ttagtgatat gggcgaataa aaagcctaac
     1381 aaggcttcgc tctctttctc gctgacatcg acaattcgcg tagtaaagcc ttcattcaca
     1441 aacagcgcct gtttaccgct caccggatgc gttcgcacca ccggatgtag caacggagga
     1501 tttttcgcga ccgcctcgcg ccagcgttga tgttcctcct cggttttgcg gtatttgtat
     1561 tccgggaacg atttacggaa atcatgctcc gcacgcagcc cgctcagcag ctggcggaag
     1621 ggaacagaga gcgcctcata ggccgcaata ccgctggtcc agagcgtatc accgccggtc
     1681 gaaggtaact ctttagctgc cagaatcgcc cctgcgggtg gcgtttcaat aaatgtcaca
     1741 tcggtatgcc agttgtcgtt atccggcgga ttatcgttat gggtatccag cacgatgatc
     1801 tcgtcaaccc cttcggcatg agggtaaaca gggtgaatat gcagttcgcc aaaacgctgg
     1861 gccagcgcgc gttgctgctg cggcgtaata gtttgatcgc gcagaaacac cacctgatgg
     1921 cgcagcaccg catggtaaag ctgttcaaac tgattatcgc ttaacgggcg cgtcaggtcg
     1981 gcacccgaaa tttgtgcgcc gatatacggc cccagcgggg taatgctcag acgttcactc
     2041 attgtacttc tccatgccag ggcgtcagac ggcgctgtaa cgcgcgcaaa cctagttcta
     2101 aaagaaaggc gataatcgca atcaccgcga ttcccgccag caccacgtca gtcgcgagaa
     2161 attcacccgc tgactgaacc ataaatccta aaccgcgcgt cgcggcaatc agctccgccg
     2221 ctaccagcgt agaccagccc acacccagac caatgcgtaa tccggtgagg atttccggca
     2281 gcgcgccggg caaaatgaca aaccacagca cctgcgcacg gctggcaccc agcgactggg
     2341 cggcacgaat gcgaacctgc tgcgcgcttt tcacccccgc cagcgccgac atcgccaccg
     2401 gtgcaaaaat cgctaaatag atcagtaaga tcttcgaggt ttcaccaata ccaaaccaga
     2461 tcaccatcag cggcaaataa gccagcggcg gcacgggacg ataaagttcg attaccggat
     2521 ccagaatgcc gcgcaccgtc gggcttagcc ccatggcgat cccgaccgga ataccgatca
     2581 ccaccgccgc gagtagcgcc agcacaatgc gcgtcaggct ggctgccaga tgctgccaca
     2641 gcgtggcgtc cataaatccc tgcggtccgg caatggtgag cagttttgcc agtacttgtt
     2701 gcggcggcgg caaaaacagc gggctaatca gttgcagcgc cgccaccgtc caccatacag
     2761 tgagtaaaac cgctaacgtg ccaatgctta aggtcacctg acgcgagagc ggccagcgcc
     2821 atttcagccg ccgcgaatgc agtttttcat taatgagcac actcatgaga acgcctcccg
     2881 ttgctcaaat acgcggctta aaacatattc gcgcatggcg atgaattgtg gatcggactt
     2941 gatgctgcgg ctcgactctc ccgcaacaaa gcggcgagca aagttgagcg gcagccgctc
     3001 cagcacacgg ccagggccgg atgaaagcag aaccagttca gtcgccataa acaccgcttc
     3061 ttctatatcg tgggtaatca acagcacctg cttgcccgtc tcctgccaga gtttcagcag
     3121 cagagtttgc atctggtcgc gggtgaaggc gtccagcgca ccaaacggtt cgtcgagtaa
     3181 taacagctgg ggattcgccg ccagcgcgcg ggcaatcccc acccgctgac gttgaccacc
     3241 ggaaagctgc cagatatagc gtttttctgc gccttccagc cccacttttt tcagcatctg
     3301 gtgcgcgatt tccagtcgct gcattttctc tatacctgcc aattgcaggc cgaacgccac
     3361 gttgtcctgt acattgcgcc acggtagtag cccttcattc tgaaaaacca ccccgcgctc
     3421 tgctcccggt ccctcaatac gcttacccgc cagttgaatg ctgccatgct gataaggcac
     3481 aaaaccggca atcagattca gcagggtggt tttaccgcag ccggacggcc ccagcaccac
     3541 cagtagctcg ccgctttcca gcgtcaggtt gatatcttcc agtactggtt tgccgccata
     3601 atcggcgtaa agatgagaga tttgcagcat ctgcgcctcc ttttattgca cgaagcgcga
     3661 ggtaacgtac tggctgtaat cattcgctac agccgggacc ttgccctgct ctttcaaaaa
     3721 ctgcgcggtg tcgatgatcg ctttgttcac cggtccggtc agttctaccg tttgttgctg
     3781 cggcgtcaga taggtattcc ccttcaccag ccccggaacg tcaccttccg gcacgccgct
     3841 taaacgcgcc agtttgctga tgttttccgg ctgtttcagc cacgcgtctg ggttagcaat
     3901 gtacggttgc tgagcatcga tggcgctttt agcgaacgct ttcacgacct caggatgttt
     3961 ctcggcaaaa tctttgcgca ccacccagac gtccagcgtt ggcgcacccc actgcccgac
     4021 ctgttcagaa tcggtcaaca ccttgccgtc tttttccagg gcgttaaccg ccggtgccca
     4081 gacataagca ccatcaatat caccccgctg ccatgcggcg ataatcgcgg gcggctgcag
     4141 gttcacaatc tccacttgcc cgggtttaat cccccagtgt ttcagcgccg ccagcaggct
     4201 gtagtgggtg gtggagataa acggtacggc aatgcgtttg ccaatcaaat cttcgggttt
     4261 gctgatagtt ttctttacca ccagcgcttc ggagttaccc agttttgacg ccagcaagaa
     4321 gacttcaatc ggcacctgtt ggctggctgc aaccgctaat gggctggaac cgaggttgcc
     4381 gatttgcacg tcgccagaag ccagcgcccg cacgatgctg gctccgctgt caaacttacg
     4441 ccagtccacg gttgctccgc tttctttagc aaaggtgttg tcggcctgag ccactttcgc
     4501 cggttcggct gaggtttgat acgccacggt gacgttcacc gcctgtgcct gaaaagcgat
     4561 gaatgccagt gcggcaagaa gtgtgttacg cgatgaaatt gccatgattg tctgctcccc
     4621 tgtcttgtta tgtgagcagt attcaggaat aaaaacattc attaaaagaa ttagtcgtta
     4681 tcgcacagat gattttattc ttagcaaaaa aacggcaaag gaaagattaa atatgcaatt
     4741 actccgttct aacaattggg aaaattacta cacttcattt tagtgatgca taaa
//
LOCUS       DAPHDW010000223         4773 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.228, whole genome shotgun sequence.
ACCESSION   DAPHDW010000223 DAPHDW010000000
VERSION     DAPHDW010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4773
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.228"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(15..398)
                     /gene="quuD"
                     /locus_tag="RH057_004316"
     CDS             complement(15..398)
                     /gene="quuD"
                     /locus_tag="RH057_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein QuuD"
                     /protein_id="HDV3130585.1"
                     /translation="MRDIQMVLERWGAWAANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMIICGCMARLKKNNSDLHDLLVDYYVVGMTFMSLAGKHCCSDGYIGKRLQKA
                     EGIIEGMLMALDIRLEMDIVVNNSN"
     gene            complement(416..511)
                     /locus_tag="RH057_004317"
                     /pseudo
     CDS             complement(416..511)
                     /locus_tag="RH057_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416078.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
     gene            506..1129
                     /locus_tag="RH057_004318"
                     /pseudo
     CDS             506..1129
                     /locus_tag="RH057_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2303 family protein"
     gene            1257..1793
                     /gene="yfdR"
                     /locus_tag="RH057_004319"
     CDS             1257..1793
                     /gene="yfdR"
                     /locus_tag="RH057_004319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HDV3130586.1"
                     /translation="MSFIKTFSGKHFYYDKINKDDIDINDIAVSLSNICRFAGHLSHF
                     YSVAQHAVLCSQLVPQEFAFEALMHDATEAYCQDIPAPLKRLLPDYKRMEEKIDAVIR
                     EKYGLPPVMSTPVKYADLIMLATERRDLGLDDGSFWPVLEGIPATEMFNVIPLAPGHA
                     YGMFMERFNDLSELRKCA"
     gene            1784..2146
                     /locus_tag="RH057_004320"
     CDS             1784..2146
                     /locus_tag="RH057_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130587.1"
                     /translation="MRMNVFEMEGFLRGKCVPRDLKVNETNAEYLVRKFDALEAKCAA
                     LENKIIPVSAELPPANESVLLFDANGEGWLIGWRSLWYTWGQKETGEWQWTFQVGDLE
                     NVNITHWAVMPKAPEAGA"
     gene            2146..2451
                     /locus_tag="RH057_004321"
     CDS             2146..2451
                     /locus_tag="RH057_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130588.1"
                     /translation="MTTFTNKELIKEIKERISSLEVRDDIERRAYEIALVSLEVEPVE
                     REAYELFMEKRFGDLVDRRRAKNGDNEYMAWDMTLGWIVWQQRAGIHFSTMTQQEVK"
     gene            2678..3841
                     /locus_tag="RH057_004322"
     CDS             2678..3841
                     /locus_tag="RH057_004322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3130589.1"
                     /translation="MSLFRRNEIWYASYSLPGGKRIKESLGTKDKRQAQELHDKRKAE
                     LWRVEKLGDLPDVTFEEACLRWLEEKADKKSLDSDKSRIEFWLEHFEGIRLKDISEAK
                     IYSAVSRMHNRKTKEIWKQKVQAAIRKGKEPPVYEPKPVSTQTKAKHLAMIKAILRAA
                     ERDWKWLEKAPVIKIPAVRNKRVRWLEKEEAKRLIDECPEPLKSVVKFALATGLRKSN
                     IINLEWQQIDMQRRVAWVNPEESKSNRAIGVALNDTACKVLRDQIGKHHKWVFVHTKA
                     AKRADGTSTPAVRKMRIDSKTSWLSACRRAGIEDFRFHDLRHTWASWLIQSGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARKIDDIFGDNVPNMSHCGIMEDIKKA"
     gene            complement(3857..3933)
                     /locus_tag="RH057_004323"
     tRNA            complement(3857..3933)
                     /locus_tag="RH057_004323"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3897..3899),aa:Arg,seq:tct)
     gene            4180..>4773
                     /gene="fimZ"
                     /locus_tag="RH057_004324"
     CDS             4180..>4773
                     /gene="fimZ"
                     /locus_tag="RH057_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria biosynthesis transcriptional regulator
                     FimZ"
                     /protein_id="HDV3130590.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFLKRIKQIQSTVKVLFLSSKSECFYAGRAIQAGANG
                     FVSKCNDQNDIFHAVQMILSGYTFFPSETLNYIKSNKCSTNSSTVTVLSNREVTILRY
                     LVSGLSNKEIADKLLLSNKTVSAHKSNIYGKLGLHSIV"
BASE COUNT         1427 a         1068 c         1108 g         1170 t
ORIGIN      
        1 aaacaattgg catattaatt agagttatta acaacgatat ccatctctaa ccggatatct
       61 aatgccatta acatcccttc aattatgccc tcagccttct gtaacctttt cccgatataa
      121 ccatcagagc agcaatgctt acctgccagt gacatgaatg tcataccgac tacataataa
      181 tctactaata aatcgtgcaa atcgctgttg ttctttttca gacgggccat gcacccgcaa
      241 atgatcatcg cgtcatcgtc acaacattgc gggcgagatt ttacttttga aggaattaat
      301 cccttaaaac cggcggcaat ggacgaccag gtcacatctt catgattatt agccgcccac
      361 gctccccaac gttcaagaac catctgaata tcacgcatta actttctcca caaaatcagg
      421 ccagcacacc aatcgccagt gcgcgatcga taaaacgaaa tatcagctcc agctgggagc
      481 catacttctc ttcaaatgcc acggtacccg ttattctaaa gatcttgcag atgaaggcac
      541 ccgctgcttt atcgatgccg ataatatgcg tgccgtcagt gtgcttaacc tgggtactat
      601 tgatgagcca ggtcacgcag ataacactgc cactctcaaa ctgaaaaaga cagcaccgtt
      661 ctctgctctg ttgtctgtta acggcgagcg taactcccag aagtcactgg cagaatggat
      721 cgaagactgg gccgactacc ttgtgggctt tgatgctaat ggtgacgcca ttcaggcaac
      781 aaaagcggct gcggcagtcc gtaaaatcac gattgaagca aaccagaccg ctgattttga
      841 agataatgac ttcagcggca aacgctccct gatggagtct gtcgaagcga agaccaaaga
      901 cattatgcca gtggcatttg aatttaaatg cgttccgttt gaaggtctga aagaacgtcc
      961 gtttaaatta cgcctcagca ttatcactgg cgatcgtcct gtactggttc tgcgcattat
     1021 tcagctggaa gcggtgcagg aagaaatggc taacgaattt cgtgatctgc ttgttgagaa
     1081 attcaaagac agcaaagtag aaacctttat tggtactttc accgcctgat ttcattactg
     1141 caaatgcccc tgcgggggca tttatggaaa cgtaattaac tcaataatcg ccggatggtg
     1201 agggcttcct tttaccagaa ttcagcgcgg tgcagtgcat atacgtggag aacaaaatgt
     1261 catttattaa aactttttcc gggaagcatt tttattatga caagataaat aaagacgaca
     1321 tcgatattaa cgatatcgcg gtttcccttt caaatatctg tcgctttgcc ggtcatcttt
     1381 cgcacttcta cagcgtcgcc caacatgcgg ttctttgcag ccagctggtg ccgcaggaat
     1441 ttgcttttga agcgttaatg catgatgcaa cagaagcgta ttgccaggac attcccgcac
     1501 cactgaaacg ccttcttcct gactataaac ggatggaaga aaaaatagac gccgtaatcc
     1561 gtgagaaata cgggttaccc ccagttatga gtacacccgt gaaatatgcc gatctcatca
     1621 tgctggcaac cgaacgccgc gatctcgggc ttgatgatgg ctctttctgg cctgtactgg
     1681 aaggcatccc ggcaacagag atgttcaacg tgattccact ggcaccgggt catgcctacg
     1741 ggatgtttat ggaacgtttt aacgatttat cggagttacg caaatgcgca tgaatgtttt
     1801 cgaaatggaa gggtttcttc gcgggaaatg tgtaccgcga gatctgaaag tgaacgaaac
     1861 aaatgctgag tacctggtac gtaaattcga cgcgcttgaa gctaaatgtg cggcactgga
     1921 aaacaaaata ataccagtgt cagctgaact gccaccagca aatgaaagtg ttctgttatt
     1981 tgatgctaat ggagaaggct ggctgattgg ctggcgttct ctctggtaca cctggggaca
     2041 aaaagaaacc ggagaatggc agtggacatt tcaggtcggg gaccttgaaa acgtcaatat
     2101 cactcactgg gcagtaatgc caaaagcacc ggaggctgga gcataatgac cacatttacc
     2161 aataaagaac tgattaaaga aatcaaagaa cgaatcagca gcctagaggt tcgagacgat
     2221 attgagcgcc gtgcttatga aatcgcactc gtatctctgg aagtagagcc agttgaacgc
     2281 gaagcctatg aattattcat ggaaaagcgt ttcggtgact tagtagatcg tcggagagca
     2341 aaaaacggcg ataacgaata catggcatgg gatatgactc tcggttggat cgtctggcag
     2401 caacgagctg gtatccattt ttcaacaatg acacagcaag aggtgaaata atggagccat
     2461 acagcctcac actcgatgag gcctgtcagt ttcttaagat atccagacca accgccacca
     2521 actggatacg aacaggccgc ctacaggcaa cacgtaaaga tccaaccaag ccaaaatctc
     2581 cttacctcac aacacggcaa gcttgcattg cggcgcttca gtctccgctg catactgtcc
     2641 aggtgagcgc gggtgatggc ataacagagg aaagaaaatg tcactcttcc gcagaaatga
     2701 aatatggtat gcctcgtatt cgctcccggg cgggaaacga attaaggaat ctcttggcac
     2761 aaaggacaag cggcaagctc aggagttgca cgacaagcga aaagcagaac tctggcgagt
     2821 agaaaagcta ggggatttac ctgatgtcac ttttgaagag gcctgcctaa gatggcttga
     2881 ggaaaaagct gataaaaaat ctctcgattc agataaaagc cggattgagt tctggcttga
     2941 acattttgag ggtataaggc ttaaagatat ctcggaggca aagatttact ctgctgtaag
     3001 cagaatgcat aacagaaaga cgaaagaaat atggaaacag aaagttcagg ccgccatcag
     3061 gaaaggtaaa gaaccgcctg tttatgaacc aaagccagta tcaactcaga caaaggcaaa
     3121 gcatcttgcc atgataaagg ccattctccg tgctgcagaa cgcgactgga agtggctgga
     3181 aaaagcgcct gtcatcaaga taccagcggt cagaaacaag cgagtcagat ggctggaaaa
     3241 ggaggaagca aaacgcctta ttgatgagtg ccccgaacca ctgaaatctg tcgtcaagtt
     3301 tgcgctggca actggtctga gaaagtcgaa catcataaat ctggaatggc aacaaatcga
     3361 catgcagcga cgagttgcct gggtgaatcc agaagagagc aaatcaaacc gcgccattgg
     3421 tgtggcgctg aacgataccg cctgtaaagt gttgcgtgat caaataggca agcatcacaa
     3481 atgggtgttt gtacatacca aggcggctaa gcgagcagat ggaacatcaa cgcctgcggt
     3541 caggaagatg cgcatcgaca gcaagacatc atggctatca gcttgtcgtc gtgcaggaat
     3601 tgaagatttc cgtttccatg acctcagaca cacctgggca agctggctga tccagtcagg
     3661 cgtcccatta tctgtgcttc aggaaatggg cggatgggag tccatagaaa tggttcgtag
     3721 gtatgctcac cttgcgccta atcatttgac agagcatgca aggaaaatag acgacatttt
     3781 tggtgataat gtcccaaata tgtcccactg tggaattatg gaggatataa agaaggcgta
     3841 actgattgaa ttgtaatggc gcgccctgca ggattcgaac ctgcggccca cgacttagaa
     3901 ggtcgttgct ctatccaact gagctaaggg cgcgttgata ccgcaatgcg gtgtaatcgc
     3961 gtgaattata cggtcaaccc ttgctgagtc aatggctttt gatctggttg ctgaacaagt
     4021 gaacgaccgc gtctgatttt ctgatttatt tcgctatagc ggcaaacaaa cacacacaca
     4081 ccgctgcgcg tctgaatcaa gaaaacccgt attttcatgt atcaaagtac aatttaccgc
     4141 cctaacggaa aattgtccgc tcctatgaga ctggtaacta tgaaaccaac gtcggtgatc
     4201 attatggata ctcatcctat catcagaatg tctattgaag ttctgttgca aaaaaacagt
     4261 gaattgcaga ttgtcctgaa aacggatgat taccgcataa ccatcgatta tctccgaacc
     4321 cgtcctgttg atttaatcat tatggatata gacttgcccg gaacagacgg ttttaccttc
     4381 ctgaaaagga tcaaacaaat ccagagcaca gtgaaagtgt tatttttatc atcgaaatca
     4441 gaatgctttt atgctggcag agcgatacaa gctggtgcta acggttttgt cagtaaatgc
     4501 aatgatcaga atgatatttt tcatgccgtt cagatgatcc tctccggata cacgtttttt
     4561 cccagcgaaa cgcttaacta tataaaaagc aataaatgta gtacgaatag ttcaacggtc
     4621 actgtgctat ctaatcgtga agtgaccata ttacgttatc tggttagcgg attatctaat
     4681 aaagaaattg ccgataagtt attacttagc aataaaacag ttagtgcgca taaatctaat
     4741 atttatggca agctaggttt gcattcaatt gta
//
LOCUS       DAPHDW010000224         4733 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.229, whole genome shotgun sequence.
ACCESSION   DAPHDW010000224 DAPHDW010000000
VERSION     DAPHDW010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.229"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(359..1963)
                     /locus_tag="RH057_004325"
     CDS             complement(359..1963)
                     /locus_tag="RH057_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV3130591.1"
                     /translation="MNPDYVQSLQTSHHFPADLTYRLFPSELAYLIDDLYESTQLPLE
                     LIFNTVLATLSLSCQSLVDVVHPHTNMPEPCSLYLLAIAEPGAGKTTINRLVMNPCYE
                     FADRLIQQYEERNKDYKTELQIWNTRQKALAANLRKVVNRGYPGEQEEEALRNHERNK
                     PTRPVRPNFIYEDVSLKALVEGLNEHPEAGVISDEAVTFFRSYLKNYPGLLNKAWSGQ
                     PFDFGRADEKYHIAPRLTFSLMSQPDVFTNYINKNDVLAWGSGFLSRFLFSQVVSSSW
                     IRDYTRGEFRTISTLENFHKKINGFLLNHNINFPGMSTERKTLKLAKKALGEWQENQI
                     KIERKALAGGEWEHIRDIVLKAGSNILRIAGIFTCYCYKDAEEIESIALFKAMHLMDW
                     YLEEASTIFYPMSARCQFEQDACELYAWIMTRIRQNNWRAIRKTDIERYGPNRLRRAE
                     KLTPVLNQLICQGYLCIIRMRSHQALYVSVYCNNGYLLPLGAMYYEQFDIVLPENIHK
                     AKAYHVNIPPELIPSFASPFSEYSLF"
     gene            2396..3052
                     /locus_tag="RH057_004326"
                     /pseudo
     CDS             2396..3052
                     /locus_tag="RH057_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082157.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(3146..3877)
                     /locus_tag="RH057_004327"
     CDS             complement(3146..3877)
                     /locus_tag="RH057_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV3130592.1"
                     /translation="MNQPIHNAYWLSRFESILNSALVQHRAVSLIRVDLRFPEYMPVT
                     IMDPDPDSAVISRFFASLKAKIQAYQRHKRRANKRVRATTLRYFWCREFGKEKGRKHY
                     HVILLLNKDTWCSPGDFTVPSSLATLIKLAWCSALHLEPWQGNGLVHFSRRTPFRKPT
                     SSDARPSSDDTPLSGGCSDTRKASDKKSGGSAVLWVKRGDVEAMQKAMERACYLVKYE
                     TKQHDGSGQRNYGCSRGPGRLLDGR"
BASE COUNT         1408 a         1119 c          972 g         1234 t
ORIGIN      
        1 atcaaacgaa gttatatatg aaaactcaca caatatggtt aatgattagc tttaatctat
       61 gttaaaaata atctttcgat tcactttcga tcaataaaaa acgaaagcca tgcttccacc
      121 agtgcgcaca ctcctgtatt caaccagctc tgacagcagg tcattatgaa aacaaaatta
      181 aatcacagat gtggtgcatg tgctgctact gttgctaccg tagcggtttt ttcagagaaa
      241 ttctcattca ccccccataa ttggtgaaca tttgcatagg tgcaggatat gcaaaacgcc
      301 ttatcaccaa cctccgaatg aaggttggtt gattttgttt tatctgtgta aggttatttc
      361 aaaataagct atactctgaa aaaggtgacg caaatgacgg aattaattcc ggtggaatat
      421 taacgtgata tgctttcgct ttatgaatat tctcgggtag aacaatatca aactgttcgt
      481 aatacattgc gcctaaagga aggaggtaac cattgttaca ataaacagaa acataaagtg
      541 cctgatgcga tcgcattctg atgatacata aatacccctg acagattaac tgattgagta
      601 caggtgtaag tttttctgct cttctcagtc gattgggacc atatctttca atgtctgttt
      661 tcctgatagc acgccaatta ttctgcctta ttcgggtcat aatccatgca tacagttcac
      721 aggcatcctg ttcaaactgg catcgtgcag acatgggata aaatattgtg ctcgcctcct
      781 ccagatacca gtccatgaga tgcatagctt taaaaagcgc aattgattca atttcctcag
      841 catctttata gcaatagcag gtgaatattc cagctatcct cagtatatta gaacctgctt
      901 tcagaacaat atctctgatg tgttcccact ccccgcctgc aagcgctttt ctttcaatct
      961 taatctggtt ttcctgccac tcccccaacg ctttcttcgc aagttttaat gtcttccttt
     1021 cggtgctcat gccggggaaa ttaatgttgt ggtttaacag gaatccgtta atctttttat
     1081 gaaagttctc cagggttgat attgttctga actcacctct cgtataatca cgtatccagg
     1141 aagaacttac aacctgactg aacagaaacc gggaaagaaa tccgcttccc cacgccagta
     1201 cgtcattttt atttatataa ttcgtaaaga catccggctg agacattaac gaaaatgtca
     1261 gacgtggcgc gatatggtat ttctcgtcag cccgtccaaa atcaaacggt tgcccactcc
     1321 atgctttatt caacaggccc ggataatttt tcagatagct tctgaaaaaa gtgaccgcct
     1381 catcagaaat aacccctgcc tcaggatgtt cattgagccc ttccacaagc gctttaagcg
     1441 aaacatcttc atagataaaa ttcggtcgaa ccggacgtgt cggtttattt ctttcgtgat
     1501 tacgcagcgc ctcttcttcc tgttcccccg gatacccccg gttaacaacc tttcttaaat
     1561 tggcagcaag cgctttctgc cgggtattcc agatctgtag ttcagtctta taatctttgt
     1621 ttctctcttc gtattgttga atgagtcgat cggcaaattc gtaacagggg ttcatcacca
     1681 gtctgtttat cgttgttttt cccgcgcctg gctctgcgat tgccaacaga taaagtgagc
     1741 agggttccgg catgttggtg tgaggatgaa caacgtcaac caatgactga caggagagtg
     1801 agagcgttgc cagtacagta ttaaaaatga gctccaacgg aagttgggta ctttcatata
     1861 agtcgtcaat gagatacgca agctcactag gaaataatct gtaggttaaa tcagctggga
     1921 agtggtggga tgtctgcaag gattgcacat aatcgggatt cattgtaatg gacctccttc
     1981 gcatactgta atcaacgcaa aacttactgt tcagttcaga aatataccga cattgtgagt
     2041 atgttcgctg acgtcgataa taaaatggct tgtatccatt caccagtccc gacgcattta
     2101 tgatgctgac aaaagcaaaa cgacatggag gtgcctcaga agtctggcac cacagcaaca
     2161 aacgatgggg catgaaacac agggaacatt gcgcccctga gcgttgagga tcttccacct
     2221 tgtatcctgg ttcttcgtta tcatgagcag cgaacagatt tggcaaagcg tatgcagcct
     2281 ggtgcccctt agggtttaag cccatatgta agcaaatggc gcatacgctg agccactcat
     2341 ctgaaacccg aacagttgtc cgtactccat aacgcattag caagaatggg caataatgag
     2401 tctgccaaat ccactttgtg ttgataccaa tgtgtccaaa acaacgctgg atattgccgc
     2461 cagcaacact attgaacggt tttctgttgc cagtggtgct gacggcgttg atttcatcat
     2521 cgctgaactg aggcagcacg atatggcctt tgtgttgatg gaaggccgcc ataacctgtg
     2581 cgtttcagcc cgaaggtttt gagatcgccg tagttaaccc caggcaagct cgtgactttg
     2641 cccgtgcgat gggatatctg gcgaagactg acccgcatga tgcgcgtttt ttttgcccag
     2701 atggctgcag ttattaatta ccatccggaa cgagaccgct ttatccggac tttgccggat
     2761 gcagaacgtc aggttcttaa tgccatagtc gtttgcagac accagttgat cgcaatgcca
     2821 gttactgagc aaaacaggtt gtacccggct cacccgcaaa gcaaaaaaaa cattaatatc
     2881 atcaaagcgc tggaagatga acacccacat ccggaaccat tttaaagaac cagctgagca
     2941 cctgagcaac attaaaggca tcggcgtaat gactacggcg gtgctggtag cctgtatgcg
     3001 caaactgctg accatcctga atgcgatact cagaaagaac aaagaaatgt aatgaatcct
     3061 gccatcaggt tactccataa ttttgttggt caagacagtt aattacgtga cgtatgctca
     3121 ggcaccggat gccgttttac agggctcacc tgccatccag tagacgcccc ggcccacgac
     3181 tgcaaccata attacgttga ccagaaccgt catgctgctt cgtctcatac ttcacgagat
     3241 aacaggctct ctccatggct ttctgcatcg cttccacatc accacgcttg acccagagca
     3301 cagcggatcc accagatttt ttgtcagaag ctttccgggt atcagaacat ccacccgaca
     3361 aaggcgtatc atcggaagaa gggcgagcat cagatgatgt cggtttacgg aaaggcgtcc
     3421 gcctggaaaa atgaaccagt ccattaccct gccagggctc gagatgcaga gcactgcacc
     3481 atgccagctt gatcagcgtc gccagcgaag aaggaacggt gaaatcccct ggcgagcacc
     3541 aggtatcttt gttgagcagt aatatcacgt gataatgttt cctgcctttt tccttgccaa
     3601 actcccgaca ccagaaataa cgcagagtgg ttgcacgcac acgcttgttg gcacgtcgtt
     3661 tatgtcgctg gtaagcctga atttttgctt tcagggatgc gaaaaaacgg gagatcaccg
     3721 cagagtccgg atcgggatcc atgatggtga caggcatata ctcagggaaa cgtaaatcca
     3781 cccggattaa cgaaacggca cggtgttgca ccagggcact gttgagaata ctttcaaaac
     3841 gggataacca gtaagcattg tgtattggtt gattcatgat gagagatctc cattgatttg
     3901 gattgacttc acatcattaa tcaccgcggt aaaactttac gaactcccac cattgagcaa
     3961 cacaacctac tccggataaa aatggaatcc cctacaaacg aaaagatggt aaaaaaacac
     4021 aacaacagca aatgaattta aaaacggcat ggacgacgag aaaagtcaga agggaaacgg
     4081 atcaacagaa cgtgcaaaca aaacatcaaa aacgcaatca gaacgatttc tggcgctgtg
     4141 aatcaacaac aacaccaaaa cctatagctt aaccacacaa ctcaatcaca gagccgaata
     4201 gcgcgtttaa aacctgttta cagataaccc caccccaaga acaaaaacca ctaacatcag
     4261 aatcgatttt ctcatcctga aaatgttacg tttcactacg caccataaaa acgattaacc
     4321 atcgataacg taaacctcat ctgaatcaaa acaccagaac caatcatcac gctttttctg
     4381 aaaagagcct tgccctgaga aaaaaacacc agggccagtt aatcatctca tcaatctcat
     4441 cgctacaaaa tcatccataa aaccatttaa ctaaatcgtc gtaagcactc cgtaaatcac
     4501 gccaggttat tcagtcggat ttgcattttt acgtaaagat acttttctcc ggttaacggc
     4561 caaaacacga caactcataa aagattaaat gtaacatcag taaaatcgaa tagcatattg
     4621 actgcttaaa caggacaggg agttttgtag tgacgttaac tgattttagc tatcgtaaaa
     4681 acagaactaa caaatttact aaactggatg tgtatcagac aaagcaatgt gta
//
LOCUS       DAPHDW010000225         4715 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.230, whole genome shotgun sequence.
ACCESSION   DAPHDW010000225 DAPHDW010000000
VERSION     DAPHDW010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4715)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4715)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4715
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.230"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            189..899
                     /locus_tag="RH057_004328"
     CDS             189..899
                     /locus_tag="RH057_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3130593.1"
                     /translation="MQTTRTPYSISFMATVLLLLLFACHSTVANAAVALGATRVIYPA
                     NQKQVLLPVTNNDPASVYLIQSWIENAGDQKDTQFVITPPLFSMQGKKENTLRIINAT
                     NHQLPGDRESLFWVNVKAIPAMEKDQKNENTLQLAIISRIKMFYRPTNLAMAPEEAPA
                     MLRFRRSGSKLTLINPTPYFITVTNMKAGNSNLPNTMVPPKGEVSVDITHAATGDISF
                     QTINDYGALTPRIKATMQ"
     gene            941..3592
                     /locus_tag="RH057_004329"
     CDS             941..3592
                     /locus_tag="RH057_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3130594.1"
                     /translation="MTAIRAAFKAYRMHQVLIMPRFARLTIALGLATAVFPVDAEYYF
                     NPRFLSNDLAESVDLSAFTKGREAPPGTYRVDIYLNDEFMTSRDITFIADDNNAELIP
                     CLSTDLLVSLGIKKSALLDNKEHSAEKHVPDNSACTPLQDRLADASTEFDVGQQHLSL
                     SVPQIYVGRMARGYVSPDLWEEGINAGLLNYSFNGNSINNRSNHNAGKSNYAYLNLQS
                     GINIGSWRLRDNSTWSYYSGSSNSSDSNKWQHINTSAERDIIPLRSRLTVGDSYTDGD
                     IFDSVNFRGLKINSTEAMLPDSQHGFAPVIHGIARGTAQVSVKQNGYDVYQTTVPPGP
                     FTIDDINSAANGGDLQVTIKEADGSIQTLYVPYSSVPVLQRAGYTRYALAMGEYRSGN
                     NLQSSPKFIQGSLMHGLEGNWTPYGGMQIAEDYQAFNLGIGKDLGLFGAFSFDITQAN
                     TTLADGTRHSGQSVKSVYSKSFYQTGTNIQVAGYRYSTQGFYNLSDSAYSRMSGYTVK
                     PPTGDTNEQTQFIDYFNLFYSKRGQEQISISQQLGNYGTTFFSASRQSYWNTSRSDQQ
                     ISFGLNVPFGDITTSLNYSYSNNIWQNDRDHLLAFTLNVPFSHWMRTDSQSAFRNSNA
                     SYSMSNDLKGGMTNLSGVYGTLLPDNNLNYSVQVGNTHGGNTSSGTSGYSSLNYRGAY
                     GNTNVGYSRNGDSSQIYYGMSGGIIAHADGITFGQPLGDTMVLVKAPGADNVKIENQT
                     GIHTDWRGYAILPFATEYRENRVALNANSLADNVELDETVVTVIPTHGAIARATFNAQ
                     IGGKVLMTLKYGNKSVPFGAIVTHGENKNGSIVAENGQVYLTGLPQSGKLQVSWGNDK
                     NSNCIVDYKLPEVSPETLLNQQTAICR"
     gene            3606..4136
                     /locus_tag="RH057_004330"
     CDS             3606..4136
                     /locus_tag="RH057_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3130595.1"
                     /translation="MKYNNVIFLGLCLGLTTYSALSADSVIKISGRVLDYGCTVSSDS
                     LNFTVDLQKNSARQFPTTGSTSPAVPFQITLSECSKGTTGVRVAFNGIEDAENNTLLK
                     LDEGSNTASGLGIEILDGNMRPVKLNDLHAGMQWIPLVPEQNNILPYSARLKSTQKSV
                     NPGLVRASATFTLEFQ"
     gene            4149..4652
                     /locus_tag="RH057_004331"
     CDS             4149..4652
                     /locus_tag="RH057_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HDV3130596.1"
                     /translation="MKRLHKRFLLATFCALFTATLQAADVTITVNGRVVAKPCTIQTK
                     EANVNLGDLYTRNLQQPGSASGWHNITLSLTDCPVETSAVTAIVTGSTDNTGYYKNEG
                     TAENIQIELRDDQDATLKNGDSKTVIVDEITRNAQFPLKARAITVNGNASQGTIEALI
                     NVIYTWQ"
BASE COUNT         1365 a         1024 c         1041 g         1285 t
ORIGIN      
        1 gttaaactta ctgttattta aaatatttac tttgtagcaa ggttttccta ttaaaagaga
       61 accaacattt attacaaacg cattaagtct ggaaacccgg cgactcgcca atgtattagc
      121 aacattaatc gattgtctga tgtaacggtt ttcccgctta tcctcttaac acatggagaa
      181 tgcatcagat gcaaactacg cgtaccccct actctatttc ctttatggcg acagtgttac
      241 ttttattgct ctttgcttgc catagcaccg ttgccaacgc agctgtcgca ctgggtgcga
      301 cgcgggtaat ttatccggca aaccagaaac aggttttatt gcctgtcacg aataatgatc
      361 ctgccagtgt ctatctcatt cagtcatgga tagaaaatgc aggtgaccaa aaagacaccc
      421 agtttgtcat taccccaccg cttttttcca tgcaaggtaa aaaagagaat acgctgcgga
      481 taatcaacgc tactaatcat cagttaccag gcgaccgtga aagcctgttc tgggtgaatg
      541 ttaaagctat tcctgctatg gaaaaagacc agaagaatga gaatacatta cagctggcaa
      601 ttattagccg cataaaaatg ttttatcgcc ccacgaacct ggcaatggca cctgaagaag
      661 cgcctgcaat gcttcggttt cgtcgcagcg gtagcaaact cacgcttatt aatccaacgc
      721 cctatttcat taccgtaaca aatatgaaag caggaaatag caatctccca aacaccatgg
      781 tgccgccaaa aggtgaagta tcggtggata tcacgcatgc tgcaaccggg gatatcagtt
      841 ttcagacgat caatgattac ggtgcattaa ctccgcgtat taaggcaacc atgcagtaag
      901 ccagtgacct aattaataat aaactcaatc ggaattaata atgacagcca ttcgtgccgc
      961 atttaaggca tatcgtatgc atcaggtgtt aataatgcct cgctttgcac ggcttaccat
     1021 tgcccttggc ttagcaactg cggtatttcc cgttgacgca gagtattatt ttaatccacg
     1081 gtttttaagc aatgatcttg ctgaatctgt cgatttatcc gcctttacta agggacgcga
     1141 ggcaccacct ggtacatatc gggtcgacat ttatttgaac gatgaattta tgactagtcg
     1201 ggatattact tttattgcag atgataataa tgcagaactc atcccatgcc tgagtacaga
     1261 tcttcttgtc agtcttggta taaaaaaatc agcgttatta gacaataaag aacattcggc
     1321 agaaaaacat gtgccggaca acagcgcatg cacaccactt caggatcgtt tggccgacgc
     1381 atcaactgag tttgatgttg gtcagcaaca tttgtcactc tccgttccgc aaatttatgt
     1441 tggcagaatg gctcgcggct atgtttctcc tgatctgtgg gaagaaggaa taaatgctgg
     1501 gcttctaaac tatagtttta atggtaattc tattaataat cgtagtaacc ataatgcagg
     1561 aaaatcaaac tatgcatatt tgaatttaca gagtggcatc aacattggta gttggcgact
     1621 gcgcgataac tcaacgtgga gttattacag tgggagtagc aattcatctg acagcaataa
     1681 atggcagcat atcaatacgt cggctgaacg tgacattatt cccttacgct cacggttaac
     1741 ggtaggtgat agttataccg atggcgatat ttttgatagt gtgaactttc gtggcctcaa
     1801 aataaattca acagaagcga tgttgcccga tagccaacat ggtttcgctc cggtgattca
     1861 tggtattgcc cgtggaaccg cacaagtgag tgtaaaacag aacggatacg atgtttatca
     1921 gactactgtt ccacccggcc cttttactat tgatgatatc aactctgcgg ccaatggtgg
     1981 tgatctgcaa gtaaccataa aagaggcaga cggcagtatt cagacattat atgttcctta
     2041 ttcgtctgtt ccggttctcc aacgtgctgg atatacgcgt tatgcgcttg ccatggggga
     2101 atatcgtagt ggaaataacc tgcaaagctc tcccaagttc atacaaggta gcttgatgca
     2161 tggtctggaa ggaaactgga caccttatgg cggaatgcaa attgcagaag attatcaggc
     2221 cttcaacctt ggtattggga aagatttagg actttttggt gccttttctt tcgatatcac
     2281 gcaggccaat acgacacttg cagatggcac ccgtcacagt gggcaatcgg ttaaatccgt
     2341 ctacagcaaa tccttctacc agacaggaac caatatccag gtcgcaggat atcgctattc
     2401 tacgcaaggt ttttataact tatccgacag tgcctacagt cgaatgagtg gttacaccgt
     2461 caagcctcct accggagaca ccaatgagca gacacaattt attgattatt ttaatctgtt
     2521 ctacagtaag cgtggtcagg aacaaataag catctctcag cagcttggaa attacggtac
     2581 gacatttttc agtgccagtc gccaaagtta ctggaacacg tcacgcagcg accagcaaat
     2641 atcatttgga ttaaatgtgc cgtttggtga tattacgact tcgctgaatt acagctattc
     2701 caataatata tggcaaaacg atcgggatca tttactcgct tttacgctta atgttccctt
     2761 cagtcattgg atgcgtacag acagtcagtc ggcatttcgt aattcaaacg ccagttacag
     2821 tatgtcaaac gatttgaaag gcggcatgac caatctatcg ggggtttatg gcactctgct
     2881 gccggataat aacctgaatt atagcgttca ggtcggtaac acccacggag gtaatacatc
     2941 gtctggcacc agtggttaca gttctcttaa ttatcgtgga gcttacggta atacgaatgt
     3001 cggttacagt cggaatggtg acagcagcca gatttattac ggaatgagtg gtgggattat
     3061 tgctcatgct gatggcatca cctttggaca gccgctgggc gacacaatgg ttctggttaa
     3121 ggctcctggc gctgataatg tcaaaataga gaaccagacc ggaattcata ccgactggcg
     3181 tggctatgca atattaccat ttgcgacaga atatagagaa aatcgtgtcg ctcttaacgc
     3241 gaattccctt gcagataatg ttgaactgga tgaaaccgta gtcactgtca tcccaactca
     3301 cggtgctatt gccagagcaa catttaatgc acaaatcggc gggaaagtat taatgacgtt
     3361 gaagtacggt aataaaagcg ttccattcgg tgcaattgtc actcacggag agaataaaaa
     3421 tggcagcatt gtcgcggaaa atggtcaggt ttatctgact ggacttccac agtcagggaa
     3481 attacaggtt tcatggggca atgataaaaa ctcaaactgt attgtcgatt acaagcttcc
     3541 tgaagtctct cctgaaacct tgctgaacca gcagacagca atctgtcgct aaggaacaat
     3601 agattatgaa atacaataac gttattttcc tcggtttatg tctggggtta accacctatt
     3661 ctgctttatc cgcagatagc gttattaaaa ttagcgggcg cgtcctcgat tatggctgca
     3721 cagtctcatc ggattcgctt aattttaccg tagatctcca aaaaaacagt gccagacaat
     3781 ttccaacgac cggtagcaca agtccagccg tcccttttca gattacgtta agtgaatgca
     3841 gcaaagggac aacgggggtt cgggttgcat ttaacggtat tgaggatgca gaaaataata
     3901 ctctgttgaa actggatgag ggaagcaata cggcctccgg tttaggtata gaaatactgg
     3961 acggaaatat gcgtccggtg aaactgaatg accttcatgc cgggatgcag tggatcccac
     4021 tggtaccaga acagaacaat attttgcctt actccgctcg tctgaagtca actcagaagt
     4081 ccgtcaatcc gggactggtg agggcttcgg caacctttac ccttgaattt caataatcag
     4141 agtcagatat gaaaagactt cacaagaggt tcctgttagc tacgttttgc gcgttattca
     4201 cagcaactct ccaggccgcc gatgtcacta tcactgttaa tggtcgggta gtcgctaaac
     4261 cctgcactat tcaaaccaaa gaagctaacg ttaatctcgg ggatctttat acgcgcaatc
     4321 tgcaacaacc tggttctgca tctggctggc acaatattac tttgtcatta accgattgtc
     4381 cggttgaaac aagtgcagtg acggcaatcg tgacaggttc aactgacaat acgggttatt
     4441 acaaaaatga aggtactgcc gaaaatattc agatagagct gagggatgac caggatgcga
     4501 cgttaaaaaa tggcgatagc aaaacggtta ttgttgatga gatcactcgt aatgcacagt
     4561 ttccacttaa ggcaagagct atcacggtga atggaaacgc aagccaggga acgatcgagg
     4621 cgctaatcaa tgtgatctac acctggcaat aacgtcgatt cgattttctt agccttcttt
     4681 ttaaatcaat tttttaacct taggatatat catgg
//
LOCUS       DAPHDW010000226         4486 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.231, whole genome shotgun sequence.
ACCESSION   DAPHDW010000226 DAPHDW010000000
VERSION     DAPHDW010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.231"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            19..492
                     /locus_tag="RH057_004332"
     CDS             19..492
                     /locus_tag="RH057_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010670508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HDV3130597.1"
                     /translation="MRIRPSSRLIILSPENHVLLFRFCHKDDALRGKTYWATPGGGLE
                     KNESFEQAAIRELFEETGLTRTLAGPQIASRTFTMMLPSGETVLAEERFFMINANTVD
                     LDRSGWSSNEKEVIRDHHWWTIEELIHTNETIFPRDLIISILENKSAGSSTLTAS"
     gene            complement(528..1322)
                     /locus_tag="RH057_004333"
     CDS             complement(528..1322)
                     /locus_tag="RH057_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3130598.1"
                     /translation="MRNVKIEDVDKLDREVVALGNDYAQGFILPQHQHRRAQLLYGAT
                     GLMYVSTCNGEWVVPPQHAVWIPPETPHAVRFVGVTTRSLYIEPTCVTGSIEYRHCKV
                     ISVSPLLRQLLLDAVDLPLMYDSARDRALVNLLLLELAEMPVREFDIPLPQHPALLAL
                     CQAFLLTPSIHDPAERWASTLFMSASTFRRLFLRQTGMSFSAWRQRACVVSALTLLIT
                     GKPVNEVALTLGYDNASSFATMFRRVTGQPPSYCRLDFKSFTGHRS"
     gene            1432..2223
                     /locus_tag="RH057_004334"
     CDS             1432..2223
                     /locus_tag="RH057_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HDV3130599.1"
                     /translation="MNDIAIIALAGFTTGITTVLFGFGGGFVVVPFVYQLMLRQSELA
                     GNAMHIAVATSTAVMIFNAGWVSYRNWRAGKLSAQTLFPLLWFIAIGAVVGSCLAGVF
                     SENIVRALFILYMLVTIGDCLLRKGFFICSSPRRLSLSVVTGGGVIIGMIAALLGVGG
                     SVMTVPLLRRHGYAMGECISASNPLSLPVALCGAVTYAVIGWQNIPVSGFLGFISLKI
                     LGLLVLTGWAGIVFSRRVIPALPDIWHARIYVILLCLVLLAMLLQ"
     gene            complement(2615..3517)
                     /locus_tag="RH057_004335"
     CDS             complement(2615..3517)
                     /locus_tag="RH057_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130600.1"
                     /translation="MDLFPDIEQAVPLPHEEVLRYMVCKICDNRSEEWLTEMCRKNNL
                     SDVCQDYLQKGVLKALEGKPDVSFYPPRLTIELVPRTCWFDNVRSAVSSTDWKRLRQQ
                     TARTAGWKCQVCGGKGPRWPVECHEIWHYDDDHQYQTLKGLIALCPSCHEVKHMGFSE
                     LRGKKDEAVAHLALVNGWSLQGAFDYVDEAFDVWRARSRHAWQLDISWLETHGIKLKD
                     ETAAEGDNIPPENQLSLELEVPPVAMESTAVSAVVEQESNSCEMGQILKDKTVSRSSS
                     AWPAFWSVCQRVFYRVKKVIREQW"
     gene            3964..4170
                     /locus_tag="RH057_004336"
     CDS             3964..4170
                     /locus_tag="RH057_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3130601.1"
                     /translation="MNNQPTRLIRLPEVIARTGYGKAWIYHLISKGRFPQPVKIGLRA
                     IAFVESEVEEWIQSVIETTRKNVA"
     gene            4185..4481
                     /locus_tag="RH057_004337"
     CDS             4185..4481
                     /locus_tag="RH057_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001019123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130602.1"
                     /translation="MNIECFVSENHMCNLGERLFNEIFDLDAIAELADDKFTREAVYM
                     LSQYLVGNTSGPFKAINMLGAHEQIHIYEIWISIIKSADELRILFKAILRHCIE"
BASE COUNT         1127 a         1103 c         1101 g         1155 t
ORIGIN      
        1 tctttttaag gggggtatgt gcgcattcga ccatcatctc gattgatcat tctttcgcct
       61 gaaaatcacg tattgctctt taggttctgt cataaagatg atgctctgag gggaaaaaca
      121 tactgggcaa cgccgggtgg agggcttgaa aaaaatgaat ctttcgagca ggccgctata
      181 cgcgaattat ttgaagaaac gggtttgaca agaacattag caggaccaca aatagcgtcc
      241 cgaaccttca caatgatgct accaagtggg gagaccgtac ttgctgaaga gcgtttcttt
      301 atgattaatg caaatacagt agatctcgac cgctcagggt ggagtagcaa tgaaaaggaa
      361 gtcattcgcg atcatcattg gtggacaata gaagagctca tacacaccaa tgagactatt
      421 ttcccacgcg atttaatcat tagcatactt gaaaacaagt cagccggatc ctcgacgtta
      481 actgcatcat gatatcccga acgtcagctt ccggcacata actgacgtta tgaacggtgc
      541 cctgtgaagc ttttgaaatc aagccgacaa tacgaaggcg gctgtcccgt gacacggcgg
      601 aacatagtgg cgaaagatga tgcattgtcg tatccgagag tgagtgccac ttcattcacc
      661 ggtttacctg ttatcagcaa cgtcagtgcg ctgacgacac aggctcgctg tcgccatgca
      721 gaaaatgaca tgccggtttg ccgtaagaaa agtcgacgaa aggtactggc gctcataaac
      781 agcgtactgg cccagcgctc agcagggtca tggattgagg gggtgagtaa aaatgcctga
      841 caaagagcca gcagagccgg atgttgtggc agcggaatat cgaattcgcg aaccggcatt
      901 tccgccagtt ccagtaacag cagatttacc agcgcacggt cgcgagcgct gtcatacatc
      961 agcggcaaat ccacggcatc aagcaacaac tgacgtaaca atggcgatac gctgatcacc
     1021 ttacagtgtc gatactcaat cgatcccgtc acgcatgttg gttcaatata taagctacgc
     1081 gtggtcacac ctacaaaccg gacagcgtga ggggtttcgg gtgggatcca tactgcatgt
     1141 tgtgggggaa caacccactc tccgttacag gttgagacgt acatgagacc cgttgcgcca
     1201 tacagcagct gagcgcggcg atgttggtgt tgtggcagta taaatccctg cgcatagtca
     1261 ttgcctaacg ccaccacttc acgatcgagc ttatcgacat cttcaatttt tacgttgcgc
     1321 acaccggtct ccgtctgaac gatttacaaa ggtaattgag cgctgcgcaa atgggaagca
     1381 ggatgctacc ggtataagct gctcgctcat tttcaggtcg gggatattct cttgaacgat
     1441 atcgcaatta ttgcactggc aggctttact accggtatca ccacggtgct ctttggtttt
     1501 gggggcggtt ttgtcgtggt gccatttgtt tatcaactga tgctgcggca gtctgagctg
     1561 gcaggaaacg ccatgcatat cgcggttgct acctcaacgg cggtgatgat atttaacgcg
     1621 ggctgggtca gctatcgaaa ctggcgcgcc ggcaaacttt cagcgcagac attgttccca
     1681 ttattatggt ttatcgccat cggggctgtc gtgggttcct gtctggcggg agtcttcagc
     1741 gagaatattg ttcgcgcact gttcattctt tatatgctgg taaccattgg cgattgtttg
     1801 ttgcgcaaag gttttttcat ctgcagttcc ccgcgtcgct tgtcactttc tgtggtaacg
     1861 ggcggcggag tgatcatcgg catgatagcc gcattgctgg gcgtgggtgg cagcgtgatg
     1921 acagtgccat tattacggcg acacggctat gcaatgggtg agtgtattag cgcttctaac
     1981 ccgctttctc tgcctgtcgc gctgtgtggt gccgtgacgt atgcagtcat tggttggcaa
     2041 aatattccgg tgagcggatt tctcggtttt atcagcctga aaattttagg cctgttggta
     2101 ctgacgggct gggctggaat agtttttagc cgtcgggtta tacctgcttt acctgacata
     2161 tggcacgcac gaatctatgt aatactgctg tgcctggtgc tactggcgat gctgctccag
     2221 taaacccatc catcgcggcg actactgggc tacctgtacc aacgtccgct cctggcacaa
     2281 agcggacact cattccaaca tacaaaaatc aatgacatat ctcctgtaca agcttttccc
     2341 ttccattgcc aacaaccaaa ccgttggccc ggaggcagca ctggtggaaa ttacggcgtg
     2401 ctctgacccg ctatgattct gaaatacgct acagtctcat catctctatc catgtaacgg
     2461 agaccggtgt cgatctgttg acgccggtac agagtaaagt ggatacactt atcgccagtc
     2521 agatcgaaat aacatcatga aaacagcgat gcattttgat ccggctctga actcattcgc
     2581 ctctctcacc gggaatgcat cgccacgggt ttattcacca ctgctcccgt atcacctttt
     2641 tcacccggta gaacactcgc tggcagactg accagaacgc tggccatgcg gacgatgagc
     2701 gggatacagt tttgtccttc aggatttgcc ccatctcgca agaattgctt tcttgttcca
     2761 cgacggcgga cactgcagtg ctctccatcg cgacaggcgg aacctcaagc tccagtgaaa
     2821 gctgattttc aggaggaatg ttgtcgccct ctgctgctgt ctcatccttg agtttaatcc
     2881 catgcgtttc gagccacgag atatccagtt gccaggcatg acgagatcgt gcccgccata
     2941 cgtcaaaagc ttcatccaca tagtcaaatg cgccctgaag agaccagccg ttgaccagag
     3001 ccagatgagc caccgcttcg tcctttttac cccggagctc gctgaacccc atatgtttga
     3061 cttcatggca tgaagggcat aacgcgatga gccctttgag ggtctgatac tgatggtcat
     3121 catcgtaatg ccagatttca tggcactcta caggccatcg gggccccttt ccgccgcaga
     3181 cctggcattt ccaccctgct gttctggccg tttgttgccg taaccgtttc cagtccgttg
     3241 aagataccgc acttctgacg ttatcgaacc agcatgttcg gggcaccagc tcaatcgtca
     3301 gtcttggtgg gtaaaagctc acatcaggtt taccctccag agccttcagc actccctttt
     3361 gaagataatc ctgacagaca tcagaaaggt tatttttcct gcacatctct gtaagccatt
     3421 cttccgaccg gttatcacag attttacata ccatgtagcg aagcacttct tcatggggta
     3481 aaggtacagc ctgttcaata tcaggaaaaa gatccacgca gaaatccttt tgcagaaggc
     3541 gtcacacctc attatacgaa aggctttgag ctgattaaac gtggtaaagc cgcgctcttc
     3601 gtccttagtc atcgtgcatt tagtgccagt gaatggccaa ttggagcacc caactgcaag
     3661 ccacaataaa tacctgaaaa gacgtaaaag cgaatgcgac agtgtcttgg tcaactaaat
     3721 ttggccttag tactggcggt ataactggtt aggattaaaa aagttgttaa tgtcctacgg
     3781 tcacccgatc acgggtagct caactctact ggcactgttc agaactgggg aagttcaggc
     3841 aacgtgaaat tccccgactc tgggcagcac caacgtcgat aacctccgtc tcaggagctt
     3901 cactcctttc ttttctcgaa gatttgtata gtagccccat cgcaacgaca aggggaaaac
     3961 aaaatgaaca atcaaccaac ccgtctcatc cgtctaccag aagtcatcgc tcgaaccggt
     4021 tacggcaagg catggatcta ccacctgatc agcaaaggtc gcttcccgca gccggtcaag
     4081 attggacttc gcgctattgc cttcgttgaa agtgaagtcg aggaatggat tcagtctgtt
     4141 attgaaacaa ccagaaaaaa tgttgcctga gcgggagagg gaaaatgaat attgaatgct
     4201 ttgtaagtga gaatcatatg tgcaaccttg gtgagaggtt gtttaacgag atttttgatc
     4261 ttgatgccat tgctgaactc gctgatgaca agtttactag agaagctgtt tatatgctga
     4321 gccagtatct tgttggaaac acgagtggac catttaaagc aatcaatatg cttggagccc
     4381 atgagcaaat tcatatttat gagatatgga tttcaattat taaatcggct gatgagttaa
     4441 ggattttatt caaggcaata cttcgacatt gtattgaatg aaatca
//
LOCUS       DAPHDW010000227         4390 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.232, whole genome shotgun sequence.
ACCESSION   DAPHDW010000227 DAPHDW010000000
VERSION     DAPHDW010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4390
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.232"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RH057_004338"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="RH057_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3130603.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="RH057_004339"
     tRNA            complement(324..399)
                     /locus_tag="RH057_004339"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="RH057_004340"
     tRNA            complement(406..480)
                     /locus_tag="RH057_004340"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="RH057_004341"
     tRNA            complement(597..681)
                     /locus_tag="RH057_004341"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="RH057_004342"
     tRNA            complement(690..765)
                     /locus_tag="RH057_004342"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="RH057_004343"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="RH057_004343"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HDV3130604.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHAVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="RH057_004344"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="RH057_004344"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HDV3130605.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQYLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="RH057_004345"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="RH057_004345"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HDV3130606.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQYATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1122 a         1103 c          984 g         1181 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctggaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagcgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc gacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ctaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa acggttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttctgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttaccgaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgcg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcgagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agcttgcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcttgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggtactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca cccaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catactgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggccat
     4201 ccgtcaggat ggccttctgc ttaatttgat gcctggcagt ttatggcggg cgtcctgccc
     4261 gccaccctcc gggccgttgc ttcgcaacgt tcaaatccgc tcccggcgga tttgtcctac
     4321 tcaggagagc gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag
     4381 cctttcgttt
//
LOCUS       DAPHDW010000228         4291 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.233, whole genome shotgun sequence.
ACCESSION   DAPHDW010000228 DAPHDW010000000
VERSION     DAPHDW010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4291
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.233"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            54..626
                     /locus_tag="RH057_004346"
     CDS             54..626
                     /locus_tag="RH057_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long polar fimbria major subunit LpfA-O113"
                     /protein_id="HDV3130607.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVHFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTAIDAEGKAEM
                     KFIARYVATNATVTAGTANADSQFTVEYKK"
     gene            674..1405
                     /locus_tag="RH057_004347"
     CDS             674..1405
                     /locus_tag="RH057_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV3130608.1"
                     /translation="MSRKSCLIRLFKTTVSCKLFTAIILSVFLGQPALTYAGVVIGGT
                     RVVYLSNNADKSISVFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHISVMDVIVDGHDVPLNMIRPFETLTLPLPAN
                     SAGGQMTWRFINDYGAVSDPIKMTL"
     gene            1430..3952
                     /locus_tag="RH057_004348"
                     /pseudo
     CDS             1430..3952
                     /locus_tag="RH057_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
     gene            3963..>4291
                     /locus_tag="RH057_004349"
     CDS             3963..>4291
                     /locus_tag="RH057_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3130609.1"
                     /translation="MNKYIKQWCFAVFMLSLSSVALAAPKGICTPDNGVFHSTLDFSG
                     YLITANENKVGTTFNTTVTNGSSYPGRCHCDTGNVGEFPYIYYTSKINQALTYAGVHS
                     NINYYDL"
BASE COUNT         1180 a          914 c         1033 g         1164 t
ORIGIN      
        1 ttaatataag tgatgattca tggtaaagga tatattatat caatggagtt tttatgaagc
       61 gtaatattat aggcggtgca ttcactctgg catctctaat gctggccggg catgcactgg
      121 cagaagatgg tgttgttcac ttcgtcggtg aaattgtcga cactacttgt gaagttacct
      181 ccgatacagc cgatcaaatt gtcccactgg gtaaagtcag taaaaatgca ttttcaggtg
      241 taggtagtct ggcgtcgcca cagaagttca gtattaagct tgaaaattgc ccggcaacgt
      301 acactcaagc agccgttcgt tttgatggta cagaagcgcc tggcggcgac ggcgacctga
      361 aagtgggtac gccgcttaca gcaggcaacc ctggtgattt taccggtaca ggacaagcga
      421 ttgcggcaac cggcgttggt attcgtattt ttaaccagtc cgataattcg caggttaaac
      481 tttataacga ctctgcttat accgctatcg atgctgaagg caaggctgaa atgaagttta
      541 ttgcacgcta tgtggcaacc aatgcgaccg taacggctgg tacggcgaac gctgattcac
      601 aatttactgt cgaatataag aaataattta tcaagggcct ttttattcag ttggctttgg
      661 ccagggagtc gttatgtcca gaaaaagttg tttaatcagg ctctttaaaa caacagtttc
      721 atgtaagtta tttaccgcga ttatactcag cgttttttta ggccaaccag cattaactta
      781 tgcaggcgtt gtcattggtg gtacgcgggt ggtttactta agtaataatg ctgataaatc
      841 tatttctgta tttagcaaag aagaaaaaat accttatctc atccaggctt gggtagatcc
      901 atttaataaa gaggataaaa gcaaagcgcc gtttaccgtt attccgccag tatcacgatt
      961 agaaccttcg caagaaaaaa tattacgcat tattcacacc aaaggtgtgt cattgcctga
     1021 tgatcgcgag tcagtcttct ggctgaatat taaaaatatt ccgccatcag cgagcaacaa
     1081 agcgacgaac tcgctggaaa tagcagtaaa aacgcgtatc aagctgttct ggcgaccagc
     1141 aaatatccgc cttattcccg aagatgctgc accgaaagtg aaatggcgcc gggaagggcg
     1201 aaatctgata gcagagaatc ccaacccaat tcatatcagt gtgatggatg tcatcgttga
     1261 tggccatgat gtgcccctca atatgatccg tccttttgaa acgctcacgt tacctttacc
     1321 tgctaattct gcgggaggcc aaatgacctg gcgatttatt aatgattacg gcgcagtcag
     1381 tgacccgata aaaatgacgc tgtaagcaag ggataaggat aggcgtatta tgatgacgac
     1441 cagaatagtg gttggcctca cggcagggac gtgtctgatt ttctcgcaaa acctgatggc
     1501 cgaggtcagt gtattcaatc cggcgcttct ggaaatcgac catcaatccg gagtcgatat
     1561 tcgccagttt aatcgggcaa acctgatgcc cccaggtgtt tatagcgttg atatttttat
     1621 caacggtaaa atgtttgaac gtcaggatgt gacatttgtt caggataatc cagatgctga
     1681 tctgcacgct tgctttattg ccattaaaaa aacactgtcc tcctttggca taaaagttga
     1741 tgcgctcaaa tcgttcaatg atgtggatga gacggtttgc ctcgatcctg ctccacgtat
     1801 tgaaggctca tcctggcagt ttgacagtga taaattgcag ctgaatatat ccattcccca
     1861 aatctacatg gacgcgatgg cttatgatta catcagcccc acgcgttggg atgaggggat
     1921 taacgcgctc accatcaact acgatttttc tggttcacat acactacgtt cagattatgg
     1981 ttcacaagag acagatacca gttatctcaa tctgcgcaat ggactgaata ttggaccgtg
     2041 gcggctacgt aattacagta ctttaaacac cagcgatggc cgtgcggaat acaactccat
     2101 tagtacctgg atacagcgcg atattgccgc gttaagaagc cagattatga ttggtgatac
     2161 gtggacggcg agcgatattt tcgacagtac gcaaattcgc ggcgcgcgtt tgtatactga
     2221 taacgatatg ctacccgcca gccagaatgg ctttgctcct gtggttcgtg ggattgcaaa
     2281 gtccaacgcc accgtcatca ttcggcagaa tggctacgtg atttatcagt ctgccgttcc
     2341 acaaggtgct tttgagatca ccgatctcaa caccgccagt acaggtggcg atttggacgt
     2401 aaccatcaaa gaagaagacg gtagcgaaca acgattcacc caaccttatg cttcattggc
     2461 gattcttaaa cgtgaaggtc agacagatgt tgatgttagc gtgggtgaat tgcgcgatga
     2521 agacggattt acaccggacg tccttcaggc gcaaatactt catggttttt cccacgggat
     2581 cactttatat ggaggtatgc aggctgctga aaattatggt tctgcagctc tgggtgtcgg
     2641 taaagatctt ggcgctttgg gcgcaatttc tttcgatgtg acacatgctc gtgcgaattt
     2701 tagccatgat gatacagaaa cgggtcagtc atatcgcttt ctctattcaa aacgatttga
     2761 cgacacagac actagcttgc gcctggttgg ctatcgttac tccaccgagg gctactatac
     2821 cctcaatgag tgggcatcgc ggcgcaacag ccctgaagac ttttgggaaa caggtaaccg
     2881 acgtagtcgc gtggagggaa cgctaacgca gtcgttgggg agagattatg gcaatttata
     2941 cctgacatta agccggcaac aatactggca taccgatgat gtcgaacgat taatgcaatt
     3001 tggctacagc agtagctgga agcgtctctc gtggaacgtc tcctagagtt attccaatac
     3061 tgccagacag gggacgggga acaaccatgc cagtgataac accagtgagc agatctacat
     3121 gctctcttta tctgttcctt tatcgggctg gtggggtaat agttacgcca cctattctgt
     3181 ttcgcaaaac gataattccg gtagctcaca tcaactcgga ctcagcggta cggcgctgga
     3241 aagaaataac ctttcatgga atttaatgca gtcctataac agtcatgatg atgaggttgg
     3301 cggtaatatg tccctgacct atgatggctc ttatggcacg gtgaacggca gctataacta
     3361 cagccaaaat tcccagaggc tgaattatgg tatcagaggg ggaattctgg cacacagcga
     3421 aggggtaacg ttaagtcagg agttaggtga aactattgct cttgttaaag cacctggggc
     3481 cgccgggtta gaaatagata atatgcgcgg tgctgcgacg gactggcgcg gctatacggt
     3541 caagacacag ctaaaccctt atgatgaaaa tcgggtagca atcagcgata actatttctc
     3601 gaagtcgaat atagaacttg ataataccgt cgttacgatg gttcccacgc gtggtgcagt
     3661 ggttaaagcg gagtttgtga ctcatgtggg ttatcgcgtt ctcttcagag tgttaaatgc
     3721 aaatggtaaa ccggtacctt ttggagccat tgctgcgata caagatgcaa gtttggcaga
     3781 ttcaggaatt gtcggtgacc gtggcgaact ttatctttct ggtctaccag aaaaaggaca
     3841 ggttacgtta tcctggggag aaaacgcctc aacaaaatgc atcttcaatt attcactttc
     3901 gacaccagaa agtgagagcg gattaattga acagggtgtg acatgtcatt aaaaggattt
     3961 ttatgaacaa gtatatcaaa cagtggtgct ttgctgtgtt tatgctctcg ttaagtagtg
     4021 tagcacttgc agctcctaaa ggtatctgta cccctgataa tggcgtattt cacagtacgc
     4081 ttgatttctc gggatatctt attaccgcga acgaaaataa ggttggaacg acatttaaca
     4141 ccactgtaac taatggatcc tcatatcctg gacgctgtca ttgtgatact ggtaacgtag
     4201 gagaattccc ctatatctat tatacgtcaa aaataaacca ggcattaact tatgctgggg
     4261 ttcactctaa tattaattat tatgatctta a
//
LOCUS       DAPHDW010000229         4207 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.234, whole genome shotgun sequence.
ACCESSION   DAPHDW010000229 DAPHDW010000000
VERSION     DAPHDW010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4207)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4207)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4207
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.234"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(45..2021)
                     /gene="speA"
                     /locus_tag="RH057_004350"
     CDS             complement(45..2021)
                     /gene="speA"
                     /locus_tag="RH057_004350"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HDV3130610.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPAE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2030..2161)
                     /gene="yqgB"
                     /locus_tag="RH057_004351"
     CDS             complement(2030..2161)
                     /gene="yqgB"
                     /locus_tag="RH057_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HDV3130611.1"
                     /translation="MKKKPVAQLERQHSLLENPCAYGLLSQFQAAIVVNCFTLNKII"
     gene            2297..2512
                     /locus_tag="RH057_004352"
     CDS             2297..2512
                     /locus_tag="RH057_004352"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130612.1"
                     /translation="MGITSAGMQSRDAECGERIFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2816..3970
                     /gene="metK"
                     /locus_tag="RH057_004353"
     CDS             2816..3970
                     /gene="metK"
                     /locus_tag="RH057_004353"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HDV3130613.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
BASE COUNT         1073 a         1142 c         1009 g          983 t
ORIGIN      
        1 ccgatttacg taaataaatc gacccgccag cagaataatc ttaattattc atcttcaaga
       61 taggtataac cgtacaaacc tgcctcgaac tcttcaagga actgttgttg cagttcagca
      121 tcaagatcgg ttttcttcac ttgatcacgg aactgagtta acagcgtttt cggatcgagc
      181 tgtacatatt gcagcatgtc cgccacggta tcgccttcgt cagacagttc cacttcgaca
      241 ctaccgtcag ggaacacgaa cacgtcaacc gcttcggtat caccgaacag gttgtgcatg
      301 ttgccgagga tctcctgata tgcgccgacc ataaagaaac cgagcatcgg cggattctct
      361 ggatcgtact ccggcatagg cattgtcgtg gcaataccgt caccatcaat atagtggtcg
      421 atagcaccgt cagagtcaca ggtaatatcc agcagcacag cgcggcgttc cggcacttga
      481 tccagccctt ccagcggcag aaccgggaac aactggtcga tcccccacgc atccggcatc
      541 gactggaaca gcgagaagtt gacgtacatt ttgtccgcca tacgttcctg cagctcgtcg
      601 ataatcggac gatgagcacg gttttgcgga tccagctgct tttgcacttc atggcacatg
      661 ctcaaataaa gctgctcagc ccatgcgcgt tcttgcaggc taaaggtgcc ggaagagtag
      721 ccgatatgaa tgtcgtgcag atccatctga ctgtcgtgta accattcacg cagagaacgg
      781 cgagttcccg gttcgtgcat ctcctgccag gtttcccaca tgctttgcag cgcacgcggc
      841 gcatcttctg caggcgcggt cggcaccgtg tattcgttac gttccacgcc gatgatatta
      901 gacaccagca cggtgtgatg cgcagtcact gcacgacccg attcagtgat taccgtcgga
      961 tgtggcagac cgttttcttc acatgcatca ccaatcgccc agatgatgtt gttggcatat
     1021 tcattgaggc cgtagttcac cgaacagtcg gactgcgaac gagtaccttc ataatccacg
     1081 cccagaccgc cgccgacgtc gaagcactga atattgacgc ccagcttgtg cagttccaca
     1141 tagaaacgcg ccgattcacg aacgcccgtg gcgatatcgc gaatattcgc catctgcgaa
     1201 ccgaggtgga agtgcagtag ttgcaggctg tcgagacgcc cggcttcacg cagggtttca
     1261 accagttgca gtacctgagt cgcagccagg ccgaacttcg atttttcccc gccggaggac
     1321 tgccatttac ccgagccctg cgaagccaga cgtgcacgca cgcccagacg aggaacgaca
     1381 ttcagacgtt ctgcttcatc cagcacaatg gcgatttctg acatcttctc aatgaccaga
     1441 tagaccttgt ggcccatctt ctcgccaatt aatgccaggc ggatatattc gcggtcttta
     1501 taaccgttgc agacgatgac gctacgggtc atgccagcat gcgccagcac cgccatcaac
     1561 tctgctttcg agcctgcttc cagccccaac ggttcgccag aatgaatcag ggactcaatc
     1621 acgcggcggt gctggttaac tttgatcgga taaacaagga agtaatcgcc gttatagccg
     1681 taggattccc gcgcacgttt gaacgcggcg ttaatggaac gcaaacggtg ctgcaggatc
     1741 tgtgggaaac agaacagtgc aggcagacgc tgaccctgtg cttcacgagt tttcactaac
     1801 tgcgcgagat cgacgcgagc ttccgggacg tccgggtccg ggcacacgct gatgtggccc
     1861 agctcgttaa cgtcatagta gttattgccc caccaggcaa tattgtaagt acgcagcatc
     1921 ttgctggctt cctgggagct cattgcaacc tcctgcatgg agcgtagtac accgtgttcg
     1981 cccgctgacg aaggcaaacc catagacatg tcgtcagaca tagcgaacct caaattattt
     2041 tattaagtgt aaaacagtta acgactatcg cagcctggaa ctgcgataac aacccataag
     2101 cacatggatt ttccagcagt gaatgctgac gctccaactg cgcaaccggt ttctttttca
     2161 taacattatt aagcacataa ccgaacgtaa gtgtgacagt tcggcgaaac cacgagaaaa
     2221 ctcttgtttt tacaagagcg cccttgttca gtcctcagta actgtaacca gctcttgaat
     2281 cctgagaagc gccgagatgg gtataacatc ggcaggtatg caaagcagag atgcagagtg
     2341 cggggaacga atcttcacca gaacggtgag acaggttaag cagcagacaa cggttcatta
     2401 tttcgtatca cctccacggc cgcctgttaa gacgaaccca caagccaaaa ctctgatttc
     2461 aacccggctg gaagtggcaa cacgaaagaa acgtcgtgtg ctttttattt aagccgcgcg
     2521 ccgcgtttta taccccacaa tggcagaaaa ttgcaaaaga taaatacgca gaatgccggc
     2581 attgtcagga aaaatttcca gccacgtttt taacagaatg agacatgatt caaaaaaaag
     2641 tggaaatcgg gtgaagaatt gacctaaaat agccatccag atgttaatcc atccataccg
     2701 attaacactc agactgccag tgtttttaac ttgcagagtc gtggtaggat ccgctaccac
     2761 agaaaatcca cacaacagtt tgagctaacc aaattctctt taggtgatat taaatatggc
     2821 aaaacacctt tttacgtccg agtccgtctc tgaagggcat cctgacaaaa ttgctgacca
     2881 aatctctgat gccgttttag acgcgatcct cgaacaggat ccgaaagcac gcgttgcttg
     2941 cgaaacctac gtaaaaaccg gcatggtttt agttggcggc gaaatcacca ccagcgcctg
     3001 ggtagacatc gaagagatca cccgtaacac cgttcgcgaa attggctatg tgcattccga
     3061 catgggcttt gacgctaact cctgtgcggt tctgagcgct atcggcaaac agtctcctga
     3121 catcaaccag ggcgttgacc gtgccgatcc gctggaacag ggcgcgggtg accagggtct
     3181 gatgtttggc tacgcaacta atgaaaccga cgtgctgatg ccagcaccta tcacctatgc
     3241 acaccgtctg gtacagcgtc aggctgaagt gcgtaaaaac ggcactctgc cgtggctgcg
     3301 cccggacgcg aaaagccagg tgactttcca gtatgacgac ggcaaaatcg ttggtatcga
     3361 tgctgtcgtg ctttccactc agcactctga agagatcgac cagaaatcgc tgcaagaagc
     3421 ggtaatggaa gagatcatca agccgattct gcccgctgaa tggctgactt ctgccaccaa
     3481 attcttcatc aacccgaccg gtcgttttgt tatcggtggc ccgatgggtg actgcggtct
     3541 gactggtcgt aaaattatcg ttgataccta cggcggcatg gcacgtcacg gtggcggagc
     3601 attctctggt aaagatccat caaaagtgga ccgttccgct gcgtacgcag cacgttatgt
     3661 tgcaaaaaac atcgttgctg ctggcctggc cgatcgttgt gaaattcagg tttcctacgc
     3721 aatcggcgtg gctgaaccga cctccatcat ggtagaaact ttcggtaccg agaaagtgcc
     3781 ttctgaacaa ctgactctgc tggtacgtga gttcttcgac ctgcgcccat acggtctgat
     3841 tcagatgctg gatctgctgc acccgatcta caaagaaacc gcagcatacg gtcactttgg
     3901 tcgtgaacat ttcccgtggg aaaaaaccga caaagcgcag ctgctgcgcg atgctgccgg
     3961 tctgaagtaa tctttcttca cctgctttca aaggccagcc tcgcgctggc ctttttcttt
     4021 tacctcaccg cggtggatag gcattcacgc cgcatccggc aaaaagctgc ccgcacaata
     4081 acatcattct tcctcaacac gttttatcgc aaattatcat cactactgaa accgattaca
     4141 ccaataacac cagccaaata tttgtaatat tttcatatta ttattcagtt ttcacagttg
     4201 ttacatt
//
LOCUS       DAPHDW010000230         4200 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.235, whole genome shotgun sequence.
ACCESSION   DAPHDW010000230 DAPHDW010000000
VERSION     DAPHDW010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4200
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.235"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(220..756)
                     /gene="yghD"
                     /locus_tag="RH057_004354"
     CDS             complement(220..756)
                     /gene="yghD"
                     /locus_tag="RH057_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GspM family type II secretion system protein
                     YghD"
                     /protein_id="HDV3130614.1"
                     /translation="MLRDKFIHYFQQWRERQLSRGEHWLAQHLAGRSPREKGMLLAAV
                     VFLFSVGYYVLIWQPLSERIEQQETMLQQLVAMNTRLRSAAPDIMAARKSATTTPAQV
                     SRVISDSASAHSVVIKRIAERGENIQVWIDPVVFNDLLNWLNALDEKYALRVTQIDAS
                     AAEKTGMVNVQRLEFGRG"
     gene            complement(758..1936)
                     /gene="gspL"
                     /locus_tag="RH057_004355"
     CDS             complement(758..1936)
                     /gene="gspL"
                     /locus_tag="RH057_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000097227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein GspL"
                     /protein_id="HDV3130615.1"
                     /translation="MSSILEIFFPLCAADPIHWQRRTPDVEHGIWSDVANEQLQQWLQ
                     TDAIRLYIPGEWISVWQVELPVVARKQIPTILPALLEEELNQDIDELHFAPLKINQQL
                     ATVAVIHQQHMRNIAQWLQENGITRATVAPDWMSIPCGFMAGDAQRVICRIDECRGWS
                     AGRALAPVMFRAQLNEQNLPLSLTVVGIAPEKLSAWAGADAERLTVTALPAITTYGEP
                     EGNLLTGPWQPRVSYRKQWARWRVMILPILLILVALAVERGVTLWSVSEQVAQSRTQA
                     EEQFLTLFPEQKRIVNLRSQVTMALKKYRPQADDTRLLAELSAIASTLKSASLSDIEM
                     RGFTFDQKRQTLHLQLRAANFASFDKLRSALATDYVVQQDALQKEGDAVSGGVTLRRK
                     "
     gene            complement(1933..2283)
                     /locus_tag="RH057_004356"
                     /pseudo
     CDS             complement(1933..2283)
                     /locus_tag="RH057_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004004479.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein GspK"
     gene            complement(2258..3058)
                     /locus_tag="RH057_004357"
     CDS             complement(2258..3058)
                     /locus_tag="RH057_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001413967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial type II/III secretion system short
                     domain protein"
                     /protein_id="HDV3130616.1"
                     /translation="MFWRDITLSVWRKKTTGLKTKKRLLPLVLAAALCSSPVWAEEAT
                     FTANFKDTDLKSFIETVGANLNKTIIMGQGVQGKVSIRTMTPLNERQYYQLFLNLLEA
                     QGYAVVPMENDVLKVVKSSAAKVEPLPLVGEGSDNYAGDEMVTKVVPVRNVSVRELAP
                     ILRQMIDSAGSGNVVNYDPSNVIMLTGRASVVERLTEVIQRVDHAGNRTEEVIPLDNA
                     SASEIARVLESLTKNSGENQPATLKSQIVADERTNSVIVSGDPPANQH"
     gene            complement(3088..3534)
                     /locus_tag="RH057_004358"
     CDS             complement(3088..3534)
                     /locus_tag="RH057_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004986340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general secretion pathway protein C"
                     /protein_id="HDV3130617.1"
                     /translation="MLHRYQGKIERLSLAEEERSTVAATNKKAVSDEAKQAVAEPAVS
                     APVEIPTAVRQALAKDPQKIFNYIQLTPVRKEGIVGYAVKPGADRSLFDASGFKEGDI
                     AIALNQQDFTDPRAMIALMRQLPSMDSIQLTVLRKGARHDISIALR"
     gene            complement(3524..4200)
                     /locus_tag="RH057_004359"
                     /pseudo
     CDS             complement(3524..4200)
                     /locus_tag="RH057_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026189.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Accessory colonization factor AcfD"
BASE COUNT          920 a         1174 c         1067 g         1039 t
ORIGIN      
        1 ttcaccaaac caaacaaaat aaaataatat caacatgata gaagcattct tcgattccga
       61 gtccggcacc actaattctt aagaacccgc ccacaaggcg ggtttttgct tttggatctg
      121 acaataacct tcacgaaaaa agtttgctta taaagtctgg gggaattact ctcgccacgt
      181 taacgagagt aattttattg atactaatct cctgatactt tacccccgtc caaactccag
      241 ccgctgcaca ttcaccatcc cagtcttctc agcagcactg gcatcaattt gtgtcacccg
      301 cagcgcatac ttttcatcca gcgcgtttaa ccagttcagc aggtcattaa acaccaccgg
      361 atctatccag acctgaatat tctccccacg ctcggctatc cgcttgatga ctaccgagtg
      421 cgcggaagcg ctgtcactga taacccgcga tacctgcgca ggcgttgttg tggcggattt
      481 tcgcgccgcc ataatatccg gcgcggcgct cctcagtctg gtgttcatcg ccaccagttg
      541 ctgcaacatt gtctcctgtt gttctatccg ttcgctcaac ggctgccaga tgaggacgta
      601 atatccgacg ctaaacagga acaccaccgc tgccagtaac atgccctttt cacgcggaga
      661 acgccccgcc aggtgttgtg ccagccagtg ttcgccacgg cttaactggc gttcacgcca
      721 ttgctgaaaa tagtgaataa atttatcgcg taacatgtta tttcctccgc aacgttacgc
      781 cgccggaaac cgcatcaccc tctttctgta atgcgtcctg ttgcacaaca taatcggttg
      841 ccagtgcact acgcagtttg tcgaaactgg caaagttagc agcccgcaac tggaggtgaa
      901 gcgtctggcg tttttgatca aaggtgaaac cacgcatttc gatgtcggaa agtgacgctg
      961 atttcagggt gctggctatc gctgacaact cggcgagcag ccgggtatcg tcggcctgtg
     1021 ggcgatattt tttcagcgcc atcgtcacct gagagcgtaa attcacaatc cgcttctgct
     1081 ccgggaacaa cgttaagaac tgttcctccg cctgggtgcg gctttgcgcc acctgttcgc
     1141 tgacgctcca taacgtcacg ccccgctcca ctgccagcgc aaccagaatc agcaatatcg
     1201 gcagaatcat cacccgccag cgcgcccact gttttcggta gctgacgcga ggctgccacg
     1261 gccctgtcag caggttccct tccggttcgc cataagtggt aatggcgggc agagctgtaa
     1321 cggtcaggcg ttcagcgtct gcgccagccc atgctgatag cttttccggt gcaatgccga
     1381 ctacggttag cgaaagcggt aaattctgct cattgagctg ggcgcggaac atgaccggag
     1441 ccagcgcccg cccggcgctc catccccggc attcatcgat gcggcagata acccgttgtg
     1501 catcgccagc cataaaccca caaggaatgg acatccagtc cggcgcgacg gtagcgcggg
     1561 tgatgccgtt ttcctgcaac cactgcgcaa tgttgcgcat atgctgttgg tgaatcacgg
     1621 ctacggttgc cagttgctga ttgattttca acggggcgaa atgcagttca tcgatatcct
     1681 ggttcagttc ttcttccagt aaggcgggca gaatagtcgg tatctgcttg cgggcgacaa
     1741 caggcagttc aacctgccag acgctgatcc attcgccggg aatgtagagt cgaatcgcat
     1801 cagtttgcag ccattgctgg agctgttcgt tagcaacgtc agaccagata ccgtgctcca
     1861 catcgggtgt acggcgctgc caatggatgg gatcagcggc gcaaagcggg aaaaaaatct
     1921 caaggatgga actcactcac tttctcctgt ctgatgccag agaaccgaaa agtgttgtgg
     1981 gcccatgcgg acaattaacg agttcatcgt cagttcaatc tcattcacgg tgatatctga
     2041 acgcagccag aagtaattgc tgtccacgct caggacggtt tttagctgtt ttttagtacg
     2101 ctcatcgacg tcagcaagta gcggctgtgc aagaaactga tcgacatctt cccagccctt
     2161 cgccggacgt tgttgtaata acgcccgcgc ctgaacaggg cttaaccacg ggtcaaacag
     2221 cgcctcaaga atcacacttt gcgtgacgtc taaagtatta atgttgattt gctggcgggt
     2281 caccactgac aatcacactg ttggtgcgtt cgtcggcgac aatttgagat ttcagcgtcg
     2341 ctggctggtt ctcgccgctg tttttcgtca ggctttccag cacgcgggcg atttccgaag
     2401 ccgaggcgtt atccagcggg atcacctctt cggtgcgatt acccgcgtga tccacgcgct
     2461 ggatcacttc cgtaaggcgc tccacaacgg aggcgcgtcc ggtgagcata atcacgttgg
     2521 agggatcgta attaacaacg ttgcctgagc ctgcgctgtc gatcatctgg cgcagaatcg
     2581 gtgccaactc gcgtaccgac acattacgta ccggcactac tttggtgacc atttcatcgc
     2641 ccgcgtagtt gtcgctgcct tcaccgacca gcggcagcgg ctcgactttc gcagcgctgg
     2701 atttcaccac cttcagcacg tcgttttcca ttggtacgac ggcatacccc tgtgcttcca
     2761 gcaggttaag gaatagctgg taatactggc gttcattgag cggggtcatg gtgcgaatac
     2821 tcactttccc ctgtacgccc tgccccataa tgatggtttt attaaggtta gcgccgacgg
     2881 tttcgatgaa cgatttcagg tcggtatctt taaaattagc ggtgaaagtg gcttcttccg
     2941 cccagaccgg tgaactgcat aatgccgctg ccagcaccag cggcagtaaa cgcttttttg
     3001 ttttgaggcc agttgtcttc ttacgccaga ccgacaacgt aatatcacgc caaaacacga
     3061 tgaatgattc tcctggatta aatgcggtta gcgcagcgcg atggaaatgt cgtggcgcgc
     3121 acccttgcgt aaaaccgtaa gttgaatgga atccattgaa ggtaactgcc gcatcagagc
     3181 aatcattgct cgtggatcag tgaaatcctg ctgatttagc gcaatggcga tatcgccttc
     3241 cttgaaaccg ctggcatcga acagagaacg atctgccccc ggcttcactg cataaccgac
     3301 aattccctcc ttacgcacag gcgtaagctg gatatagtta aaaattttct gcggatcttt
     3361 cgccagtgcc tgacgcacgg cagtcggtat ctcaactggc gcactgacag caggttcagc
     3421 aacagcttgc tttgcttcgt cactgacagc ttttttgttg gtcgcggcaa cggtggaacg
     3481 ctcctcttca gccaggctca ggcgctctat ttttccctga taccggtgaa gtatgagtgg
     3541 atggcgcttt atcggtcgcg gcattgtttt ccacctcttc attgaccagc tttttgaact
     3601 cttccggtgc cagatcgatt tgcttataca gcgagtcgaa acgtttactc tcgatcaccg
     3661 aggagaactc caggcacacc tgttctgtat tcgccgggca actgttcatg gaggtgacca
     3721 gcgagagcgc attgcttttc ttgttgtcgg aacccgccag ttcttgcgcg tcctcaagac
     3781 taaacgacac tttttcaacc gcacgcaggc tacgcgcagc ttctgactgg gtgtcgaagg
     3841 tagcaattgt cgtgttgttc cctgccacac aggtgacttt gtcgcctggt gtaaaggtaa
     3901 agccatcgct ggattcatca ttacaggtga tatcaccagt tacccgcagg cttccgccca
     3961 gagtcagata acccgttttc gtaggaacag gttctggctc cggttctggt gttggctcag
     4021 gatcaggtgt cggttccggc gtaggttctg ggtccggcgt tggctcaggc gtcggctccg
     4081 ggtttggtgt tggatcaggt ttcacttccg gcaaagaccc tgttccagaa tctacaggcg
     4141 gcgtatcgga ggaagatccg gagccaccgc catcacaacc ggctaacagg gttgcactca
//
LOCUS       DAPHDW010000231         4192 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.236, whole genome shotgun sequence.
ACCESSION   DAPHDW010000231 DAPHDW010000000
VERSION     DAPHDW010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4192
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.236"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(142..1227)
                     /gene="dmlA"
                     /locus_tag="RH057_004360"
     CDS             complement(142..1227)
                     /gene="dmlA"
                     /locus_tag="RH057_004360"
                     /EC_number="1.1.1.83"
                     /EC_number="1.1.1.85"
                     /EC_number="1.1.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional
                     D-malate/3-isopropylmalate/tartarate dehydrogenase"
                     /protein_id="HDV3130618.1"
                     /translation="MMKTMRIAAIPGDGIGKEVLPEGIRVLQAAAERWGFALSFEQME
                     WASCEYYSHHGKMMPDDWHEQLSRFDAIYFGAVGWPDTVPDHISLWGSLLKFRREFDQ
                     YVNLRPVRLFPGVPCPLAGKQPGDIDFYVVRENTEGEYSSLGGRVNEGTEHEVVIQES
                     VFTRRGVDRILRYAFELAQSRPRKTLTSATKSNGLAISMPYWDERVEAMAENYPEIRW
                     DKQHIDILCARFVMQPERFDVVVASNLFGDILSDLGPACTGTIGIAPSANLNPERTFP
                     SLFEPVHGSAPDIYGKNIANPIATIWAGAMMLDFLGNGDERFQQAHNGILAAIEEVIA
                     HGPKTPDMKGNATTPQVADAICKIILR"
     gene            1330..2253
                     /gene="dmlR"
                     /locus_tag="RH057_004361"
     CDS             1330..2253
                     /gene="dmlR"
                     /locus_tag="RH057_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310535.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DmlR"
                     /protein_id="HDV3130619.1"
                     /translation="MNNLPLLNDLRVFMLVARRAGFAAVAEELGVSPAFVSKRIALLE
                     QTLNVVLLHRTTRRVTITEEGERIYEWAQRILQDVGQMMDELSDVRQVPQGMLRIISS
                     FGFGRQVVAPALSALAKAYPQLELRFDVEDRLVDLVNEGVDLDIRIGDDIAPNLIARK
                     LATNYRILCASPEFIAQHGAPKHLTDLSALPCLVIKERDHPFGVWQLRNKEGHHAIKV
                     TGPLSSNHGEIVHQWCLDGQGIALRSWWDVSENIASGHLVQVLPEYYQPANVWAVYVS
                     RLATSAKVRITVEFLRQHFAEHYPNFSLEHA"
     gene            2380..3018
                     /gene="leuE"
                     /locus_tag="RH057_004362"
     CDS             2380..3018
                     /gene="leuE"
                     /locus_tag="RH057_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine efflux protein LeuE"
                     /protein_id="HDV3130620.1"
                     /translation="MFAEYGVLNYWTYLVGAIFIVLVPGPNTLFVLKNSVSSGMKGGY
                     LAACGVFIGDAVLMFLAWAGVATLIKTTPILFNIVRYLGAFYLLYLGSKILYATLKGK
                     NNEAKSDEPQYGAIFKRALILSLTNPKAILFYVSFFVQFIDVNAPHTGISFFILATTL
                     ELVSFCYLSFLIISGAFVTQYIRTKKKLAKVGNSLIGLMFVGFAARLATLQS"
     gene            3191..3550
                     /gene="yeaR"
                     /locus_tag="RH057_004363"
     CDS             3191..3550
                     /gene="yeaR"
                     /locus_tag="RH057_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1971 domain-containing protein"
                     /protein_id="HDV3130621.1"
                     /translation="MLQIPQNYIHTRSTPFWNKQTAPAGIFERHLDKGTRPGVYPRLS
                     VMHGAVKYLGYADEHSAEPDQVILIEAGQFAVFPPEKWHNIEAMTDDTYFNIDFFVAP
                     EVLMEGAQQRKVIHNGK"
     gene            3554..3742
                     /locus_tag="RH057_004364"
     CDS             3554..3742
                     /locus_tag="RH057_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1869 domain-containing protein"
                     /protein_id="HDV3130622.1"
                     /translation="MGKATYTVTVTNNSNGVSVDYETETPMTLLVPEVAAEVIKDLVN
                     TVRSYPVRRKTPSFRAGI"
     gene            complement(3761..4189)
                     /gene="tnpA"
                     /locus_tag="RH057_004365"
                     /pseudo
     CDS             complement(3761..4189)
                     /gene="tnpA"
                     /locus_tag="RH057_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313209.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         1064 a          998 c         1049 g         1081 t
ORIGIN      
        1 ataaattaaa caattaagaa tacggaaagc cataaaacac gaaacggaaa caataaaagg
       61 tgaaatgacg caggttattt actgcaaaga acgaataaaa aaaccgcgtc agtgacgcgg
      121 ttcaaataaa ctggtttgac cttaacgcaa aataattttg caaatcgcgt cggcaacctg
      181 tggcgtggtg gcattgcctt tcatatcagg tgttttcggc ccgtgagcaa tcacttcttc
      241 aattgctgcc agaataccgt tatgcgcttg ctggaaacgc tcatcgccat tgccgagaaa
      301 atcgagcatc attgcaccgg cccaaatcgt ggcgataggg ttagcaatgt ttttcccgta
      361 gatatccggc gcggaaccgt ggactggctc gaagagcgac gggaaagtgc gttccggatt
      421 caggttggcg gatggggcaa tgccaatggt gccggtgcag gccgggccaa gatcggaaag
      481 gatatcgcca aataaattgg acgccaccac cacatcgaag cgttccggct gcatcacaaa
      541 acgcgcgcag agaatatcaa tatgctgctt gtcccagcgg atctccgggt aattctcggc
      601 cattgcttcc actcgctcat cccagtacgg catgctgatg gctaaaccgt ttgatttagt
      661 ggcagaagtt agcgtcttac gtgggcggct ttgcgcaagt tcgaaggcat aacgcaaaat
      721 gcgatcgaca ccacgacgcg taaataccga ttcctgaatg acgacttcat gctctgtacc
      781 ttcattcact ctaccgccga gcgaggaata ttcgccttcg gtgttttccc tgaccacgta
      841 aaaatcgatg tcgcctggct gttttcccgc cagcgggcag ggaacgccag gaaagagacg
      901 aaccgggcgc aggttgacgt actggtcgaa ttcacgacga aatttcagca gcgaacccca
      961 caacgaaata tggtcaggaa cggtgtccgg ccagccgacg gcaccaaaat agatggcgtc
     1021 gaaacggcta agttgctcat gccagtcgtc cggcatcatt ttaccgtgat ggctgtaata
     1081 ctcgcagctc gcccactcca tttgctcaaa actcaaggcg aagccccagc gctcagcggc
     1141 agcctgtaac acgcgaatcc cttcaggaag gacttctttg ccaatcccgt ctcccgggat
     1201 cgcagcaata cgcatcgttt tcatcattgg gttctcactt cctgaccatc aaaattgaca
     1261 acatcaccat cctataattg actgaaaagg aattaatccc cgtaactggt gaaacataaa
     1321 acacgaatca tgaataattt accgctgctg aatgatttgc gcgtctttat gctggtggct
     1381 cgtcgggctg gttttgccgc cgtggcggaa gaactgggcg tttcaccggc gttcgtcagc
     1441 aagcgcatcg ccttgctgga gcaaacgcta aacgtggtgt tgttacaccg caccacccgc
     1501 cgcgtcacca ttaccgaaga aggagagcgg atttatgaat gggcgcagcg gattttgcag
     1561 gatgtcggtc agatgatgga cgaactttct gacgtgcgtc aggtgccgca ggggatgctg
     1621 cgtattatca gcagctttgg ttttggacga caggtggtgg ctccggcgtt atcagcgctg
     1681 gctaaggcgt atccgcaact ggaattacgt tttgatgtgg aagaccggtt ggtcgatttg
     1741 gtcaatgaag gtgttgatct cgatattcgc attggcgatg acatcgcccc taatttaatc
     1801 gcccgcaaac tggcgactaa ttaccgcatt ctctgcgcgt cgccggaatt tattgcgcaa
     1861 catggcgcgc cgaaacattt aaccgatctt tccgcattgc cgtgcctggt cattaaagag
     1921 cgcgatcatc cttttggtgt ctggcaatta cgcaataaag aaggccacca tgcgattaaa
     1981 gtcaccggcc cactgtcgtc taatcatgga gaaattgtcc atcagtggtg tctcgatggg
     2041 caggggattg ctctgcgctc ctggtgggat gttagcgaaa acattgccag tggtcattta
     2101 gtgcaagtgc taccggaata ttaccagcca gcgaacgtct gggccgttta tgtttcaagg
     2161 ctggcgacgt cagcgaaagt gcggataacg gtagaatttt tacgccagca ttttgccgag
     2221 cactacccga atttttcact ggagcatgcc tgattaatga ttcaattatc gggttgatat
     2281 cgggttaaaa cctgattttc tcctttctaa gccgctacag attggttagc atattcacct
     2341 ttaatcgcgc atgatcgaaa gataattaaa gaggttaatg tgttcgctga atacggggtt
     2401 ctgaattact ggacctatct ggttggggcc atttttattg tgttggtgcc agggccaaat
     2461 accctgtttg tactcaaaaa tagcgtcagt agcggtatga aaggcggtta tcttgcggcc
     2521 tgcggtgtat ttattggcga tgcagtattg atgtttctgg catgggctgg agtggcgaca
     2581 ttaattaaga ccaccccgat attattcaac attgtacgtt atcttggtgc gttttatttg
     2641 ctctatctgg ggagtaaaat tctctacgcg accctgaagg gtaaaaataa cgaggccaaa
     2701 tccgatgagc cccaatacgg ggccattttt aaacgcgcgt taattttgag cctgactaat
     2761 ccgaaagcca ttttgttcta tgtgtcgttt ttcgtacagt ttatcgatgt taatgcccca
     2821 catacgggaa tttcattctt tattctggcg acgacgctgg aactggtgag tttctgctat
     2881 ttgagcttcc tgattatttc tggtgctttt gtcacgcagt acatacgtac caaaaagaaa
     2941 ctggctaaag ttggcaactc actgattggt ttgatgttcg tgggtttcgc cgcccgactg
     3001 gcgacgctgc aatcctgata cattcagccc gcgttgtcgc gggcttccca cttgtaatcc
     3061 tccctgattt ttctctgata tggtgctaaa aagtaaccaa taaatggtat ttaaaatgca
     3121 aattatcagg cgtaccctga aacggctgga ataaaccgtt ttcagcgcat tccccgaagg
     3181 agggaatagg atgctgcaaa tcccacagaa ttatattcat acgcgctcaa cgcctttctg
     3241 gaataaacaa actgcgcctg ccggaatatt cgaacgtcat cttgataaag gaacgcgccc
     3301 gggggtttac ccacgccttt ccgttatgca tggggcggtc aaatatctcg gctacgctga
     3361 tgaacacagt gcagagcctg atcaggtgat ccttatcgaa gcggggcagt ttgcggtgtt
     3421 ccctccagaa aagtggcaca acattgaagc catgactgac gatacttatt tcaacattga
     3481 cttcttcgtg gctcctgaag tcctgatgga aggtgcgcaa caacggaaag tcattcataa
     3541 cgggaaatga atcatgggca aagcaacgta taccgtgacc gtcaccaata acagcaatgg
     3601 cgtttctgtc gattatgaaa cagagacgcc gatgactttg ctggtgccag aagtggcggc
     3661 tgaagtgata aaagatctgg tgaataccgt gcgttcttat ccggtgcgcc gtaaaacccc
     3721 gtccttcagg gcggggatat aaggcgcggt tttccacctg accaggtgtt tgctgttgtt
     3781 caatgtattg gcggatgacg gatattggcg cacctccgca gctactggca aaatagccag
     3841 gactccacaa aacgcctttg tagtaatacc tgactgcaat atctggtcga tctcgtcgca
     3901 gtaacctacc cgatacgcct ttgaggctgt ttaccagact ggatatcgcc agtttgggag
     3961 gatagttaat taacaaatgg acgtgatctg gttcgccatc catttcaaca agttcagctt
     4021 caaaatcagc acatacattt gaaaagtaag tgcgtagttt ttctgtcgcg tcatggtcaa
     4081 aaatctggag tcggtatctg gtgacaaaga ccaggtgaac atgcatcagg aaaacacaat
     4141 gccttccacg ccggatatcg gtttcttttt tcacagacca aagtatcatt tg
//
LOCUS       DAPHDW010000232         4190 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.237, whole genome shotgun sequence.
ACCESSION   DAPHDW010000232 DAPHDW010000000
VERSION     DAPHDW010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.237"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(199..771)
                     /locus_tag="RH057_004366"
     CDS             complement(199..771)
                     /locus_tag="RH057_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Polarity suppression protein"
                     /protein_id="HDV3130623.1"
                     /translation="MESTALQQAFDTCQNNKAAWLQRKNELAAAEQEYLRLLSGEGRN
                     VSRLDELRNIIEVRKWQVNQAAGRYIRSHEAVQHISIRDRLNDFMQQHGTALAAALAP
                     ELMGYSELTAIARNCAIQRATDALREALLSWLAKGEKINYSAQDSDILTTIGFRPDAA
                     SVDDSREKFTPAQNMIFSRKSAQLASRQSV"
     gene            complement(845..1126)
                     /locus_tag="RH057_004367"
     CDS             complement(845..1126)
                     /locus_tag="RH057_004367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ogr/Delta-like zinc finger family protein"
                     /protein_id="HDV3130624.1"
                     /translation="MKRPKRMLVTGYCCRRCKGLALSRTSRRLSQEVTERFYVCTDPG
                     CGLVFKTLQTINRFIVRPVTPDELAESLHEKQELPPVRLKTQSYSLRLE"
     gene            1151..1465
                     /locus_tag="RH057_004368"
     CDS             1151..1465
                     /locus_tag="RH057_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5375 domain-containing protein"
                     /protein_id="HDV3130625.1"
                     /translation="MAVPPVLRAALYRRAVACAWLTVCERQHRYPHLTLESLEAAIAA
                     ELEGFYLRQHGEEKGRQIACALLEDLMESGPLKAAPSLSFLGLVVMDELCARHIKAPV
                     LH"
     gene            1480..3813
                     /locus_tag="RH057_004369"
     CDS             1480..3813
                     /locus_tag="RH057_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000783678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HDV3130626.1"
                     /translation="MKMNVTATVSHALGHWPRILPALGIQVLKNRHQPCPVCGGSDRF
                     RFDDREGRGTWYCNQCGAGDGLKLVEKVFGVSPSDAAAKVAAVTGCLPPADPAVTAAA
                     GAETDAARKNAAALAQTLMAKTRPGTGNAYLTRKGFPGRECRMLTGTHRAGGVSWCAG
                     DLVVPLYDDSGELVNLQLISADGRKRTLKGGQVRGTCHTLEGQNQAGKRLWIAEGYAT
                     ALTVHHLTGETVMVALSSVNLLSLASLARQKHPACQIVLAADRDLSGDGQTKAAAAAD
                     ACEGVVALPPVFGDWNDAFTQYGGEATRKAIYDAIRPPAESPFDTMSEAEFSAMSTSE
                     KAMRIYEHYGEALAVDANGQLLSRYENGVWKVLPPQDFARDVAGLFQRLRAPFSSGKV
                     ASVVDTLKLIIPQQEAPSRRLIGFRNGVLDTQSGTFHPHSPSHWMRTLCDVDFTPPVE
                     GETLETHAPAFWRWLDRAAGGRAEKRDVILAALFMVLANRYDWQLFLEVTGPGGSGKS
                     IMAEIATLLAGEDNATSATIETLESPRERAALTGFSLIRLPDQEKWSGDGAGLKAITG
                     GDAVSVDPKYRDAYSTHIPAVILAVNNNPMRFTDRSGGVSRRRVIIHFPEQIAPQERD
                     PQLKDKITRELAVIVRHLMQKFSDPMLARSLLQSQQNSDEALNIKRDADPTFDFIGYL
                     ETLPQTSGMYMGNASIIPRNYRKYLYHAYLAYMEANGYRNVLSLKMFGLGLPVMLKEY
                     GLNYEKRHTKQGIQTNLTLKEESYGDWLPKCDDPATT"
BASE COUNT          908 a         1213 c         1181 g          888 t
ORIGIN      
        1 cagccttagc gcgcaatgct ccccccgcca cgcccgcccg ctttgcgggg cggttttaat
       61 gcagttgcac tgacacgctc agaccgcgcc gggaatggcg cggtctgcag aaaatgaggc
      121 agggaaacgc atgcaaagcc atgcacctta tcgatgcatg gcttttttca gtaaaaacgg
      181 gcggattttc ggggaatttt acacagactg acgtgatgcc agttgcgcac ttttacgcga
      241 aaaaatcatg ttctgcgcag gggtgaattt ttcacggctg tcatccaccg aagccgcgtc
      301 aggcctgaat ccgatggtcg ttaaaatgtc gctatcctgt gcggaataat taattttttc
      361 ccccttcgca agccaggaca gaagggcttc acgcagggca tctgtggcac gctgtatggc
      421 acagtttcgg gcaatggccg tcagctcact gtagcccatc agctccggtg ccagtgccgc
      481 cgccagtgct gtgccgtgct gctgcataaa atcattcagc cggtcgcgga tgctgatgtg
      541 ctgaacggct tcatgcgaac gaatataacg accggcggcc tgattcacct gccattttct
      601 gacttcgata atattgcgta attcgtccag gcggctgacg tttctgcctt ctcctgacag
      661 aagccgcaga tattcctgtt cggccgctgc cagctcattt ttgcgttgca gccatgctgc
      721 tttgttattc tgacaggtgt caaaggcctg ctgtaaggct gtgctttcca tcgttatctc
      781 tttctcatca tgctgaagaa taaaaatacg gtgtgcggcg acggccggtg ttaaccggca
      841 gccctcattc cagacgcagc gaatatgatt gtgtttttaa gcgtactggc ggcagttcct
      901 gtttttcatg caggctttct gccagttcgt ccggcgtgac cgggcggaca atgaagcggt
      961 tgatggtctg aagcgtttta aacaccagac cacagcccgg atccgtgcac acataaaaac
     1021 gctcggtgac ttcctgagac agacgccgcg atgttcttga cagtgcaagg cctttacatc
     1081 tccgacaaca atatccggta acaagcattc ttttcgggcg tttcatactg ccggaggctg
     1141 acgtcagtga atcgcggtac cgcccgtctt acgcgctgcc ctttaccgtc gcgctgtcgc
     1201 ctgtgcctgg ctgaccgtgt gcgaacgtca gcaccgctac ccgcatctca cccttgagtc
     1261 actggaggcg gccatcgccg ctgagctgga gggcttttat ctgcgccagc acggtgagga
     1321 aaaagggcgt cagatagcct gtgccctgct ggaagattta atggaatccg gccccctgaa
     1381 ggccgcgccg tcgctgtcct ttctcgggct ggttgtgatg gatgaactct gtgcccgtca
     1441 cataaaagcg ccggtactgc actgaaggag aacaacacca tgaaaatgaa cgtaaccgcc
     1501 accgtcagcc atgcgctcgg ccactggccg cgtattctcc cggcgctggg gattcaggtg
     1561 ctgaaaaacc gtcatcagcc ctgtccggtc tgtggcggga gtgaccgctt ccgttttgat
     1621 gacagggagg ggcgcggcac ctggtactgc aatcagtgtg gtgccggtga cggcctgaaa
     1681 ctggttgaaa aggtgtttgg tgtttccccg tccgacgcgg ccgcaaaggt ggctgccgtg
     1741 accggctgtc tgcccccggc tgacccggca gtgacggccg ccgccggtgc tgaaacagac
     1801 gctgcccgga agaacgccgc cgcactggca caaaccctga tggcgaaaac ccgtcccgga
     1861 accggtaacg cctacctgac ccgcaagggt tttcccggcc gggaatgccg gatgctgacc
     1921 ggcacacaca gagccggtgg cgtgagctgg tgcgccggtg accttgtggt gccactgtat
     1981 gacgacagcg gcgaactggt taaccttcag ttaatcagcg ctgacggccg taagcgcacc
     2041 ctgaaaggcg gacaggtcag gggcacctgt cacacccttg aaggacagaa tcaggccgga
     2101 aaacgtctgt ggatagcgga gggatacgcg accgcactta ccgtgcatca cctgaccggt
     2161 gaaacggtga tggtggcgct ttcttccgtg aacctccttt ctctggccag ccttgcccgg
     2221 cagaagcatc cggcctgtca gattgtcctt gctgctgacc gtgacctcag cggtgacggc
     2281 cagacaaaag ccgccgcagc cgcagatgcg tgtgaaggtg ttgttgccct gccgccggtc
     2341 ttcggtgact ggaatgatgc cttcacgcag tacggcgggg aagccacccg taaggccatt
     2401 tatgatgcca tccggccacc ggctgaaagc ccgttcgaca ccatgagcga agcggagttt
     2461 tccgccatga gtaccagcga aaaggccatg cgtatctatg agcattacgg cgaggcgctc
     2521 gcggtcgatg ccaacggcca gcttctgtcc cgctatgaaa atggtgtctg gaaggtgctg
     2581 ccgccacagg actttgcccg ggatgtggcc gggctgtttc agcgtctgcg cgcgccgttc
     2641 tcctccggga aggtggcctc cgtggtggac accctgaagc tgattattcc gcagcaggaa
     2701 gccccctccc gccgcctgat tggctttcgt aacggcgtgc tcgacacgca gagcggcacg
     2761 ttccacccgc acagtccgtc acactggatg cgcaccctgt gcgatgtgga tttcaccccg
     2821 ccggtggaag gggaaacgct ggaaacccac gcccccgcgt tctggcgctg gcttgaccgt
     2881 gccgccggtg gtcgtgcgga aaaacgcgac gtgattctgg ccgcactgtt tatggtgctg
     2941 gcaaaccgct acgactggca gctctttctg gaggtgaccg gtcccggcgg cagcggcaaa
     3001 agtatcatgg ccgaaatagc caccctgctg gccggggagg ataacgccac gtcggccacc
     3061 atcgagacgc tggaatcccc gcgtgaacgt gccgcgttaa ctggcttctc actgatacgc
     3121 ctgccggacc aggaaaaatg gagcggcgac ggtgccggac tcaaggccat caccggcggc
     3181 gatgcggtgt ccgttgaccc gaaataccgg gatgcatact ccacgcatat cccggcggta
     3241 attctggccg tgaacaataa cccgatgcgc ttcaccgacc gcagcggcgg cgtgtcacgc
     3301 cggcgggtga ttattcactt cccggaacag atagccccgc aggagcgcga cccgcagctt
     3361 aaggacaaaa tcacccgcga gctggcggtc atcgtgcgtc acctgatgca gaagttcagc
     3421 gacccgatgc tcgcccggtc actgcttcag tcccagcaga actcagacga ggcgctgaac
     3481 atcaaacggg atgccgaccc gacgtttgat tttatcggct atctggaaac cctgccgcag
     3541 accagcggca tgtatatggg gaacgccagt atcatcccgc gcaattaccg taaatacctc
     3601 tatcacgcct atctggccta catggaggca aacggctacc ggaatgtact cagtctgaaa
     3661 atgttcgggc tggggctgcc ggtgatgctg aaggaatacg gactgaatta cgagaagcgc
     3721 cataccaaac aggggataca gaccaacctg acgctgaaag aggaaagcta cggcgactgg
     3781 ctgccaaaat gtgacgaccc tgcgacaacc tgaccatcat gactcatctg accggcatct
     3841 gccggtcttt ttttatccct gaattccccg aaggtgaaca atccactgtt cacccttcac
     3901 cgtgtattca cccgttatca cactgaaatt aaaagagaaa aacgaaaggt gaacagtgtg
     3961 aacaatcaaa tcaaaaaaaa cttttttttc tccctgtgtg atttcagtat gggggattaa
     4021 tcaccggtat gagtcacacc ggcagaatgc cggaggtgaa gaatcgaatg ttcacccttc
     4081 acccattatt caccacctat cactctgaaa taaaaggaga caaaagaaag gtgaacagtg
     4141 tgaacagttc tttcgaaaaa aaactttttt cttactttta attgtgtaaa
//
LOCUS       DAPHDW010000233         4142 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.238, whole genome shotgun sequence.
ACCESSION   DAPHDW010000233 DAPHDW010000000
VERSION     DAPHDW010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4142)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4142)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4142
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.238"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            60..>4142
                     /gene="rhsD"
                     /locus_tag="RH057_004370"
     CDS             60..>4142
                     /gene="rhsD"
                     /locus_tag="RH057_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhs element protein RhsD"
                     /protein_id="HDV3130627.1"
                     /translation="MSGKPAARQGDMTQYGGPIVQGSAGVRIGAPTGVACSVCPGGMT
                     SGNPVNPLLGAKVLPGETDLALPGPLPFILSRTYSSYRTKTPAPVGVFGPGWKAPSDI
                     RLQLRDDGLILNDNGGRSIHFEPLLPGEAVYSRSESMWLVRGGKAAQPDGHTLARLWG
                     ALPPDIRLSPHLYLATNSAQGPWWILGWSELVPGAEDVLPAPLPPYRVLTGLADRFGR
                     TLTYRREAAGDLAGEITGVTDGAGREFRLVLTTQAQRAEEARKQRTASLSSPDTPRPL
                     SASAFPDTLPGTEYGPDRGIRLSAVWLMHDPAYPESLPGAPLARYTYTEAGELLAVYD
                     RSNTQVRAFTYDAQHPGRMVAHRYAGRPEMRYRYDDTGRVVEQLNPAGLSYRYLYEQD
                     RITVTDSLNRREVLHTEGGAGLKRVVKKELADGSVTRSGYDAAGRLTAQTDAAGRRTE
                     YGLNVVSGDITDITTPDGRETKFYYNDGNQLTAVVSPDGLESRREYDEPGRLVSETSR
                     SGETVRYRYDDAHSELPATTTDATGSTRQMTWSRYGQLLAFTDCSGYQTRYEYDRFGQ
                     MTAVHREEGISLYRRYDNRGRLTSVKDAQGRETRYEYNAAGDLTAVITPDGNRSETQY
                     DAWGKAVSTTQGGLTRSMEYDAAGRVISLTNENGSHSVFSYDALDRLVQQGGFDGRTQ
                     RYHYDLTGKLTQSEDEGLVTLWHYDASDRITHRTVNGDPAEQWQYDGHGWLRDISHLS
                     EGHRVAVHYGYDDKGRLTGERQTVENPETGELLWQHETKHAYNEQGLANRVTPDSLPP
                     VEWLTYGSGYLAGMKLGGTPLVEYTRDRLHRETVRSFGSRAGSNAAYELTSTYTPAGQ
                     LQSQHLNSLVYDRDYGWNDNGDLVRISGPRQTREYGYSATGRLESVRTLAPDLDIRIP
                     YATDPAGNRLPDPELHPDSTLTVWPDNRIAKDAHYVYHYDEYGRLTEKTDRIPAGVIR
                     TDDERTHHYHYDSLHRLVHYIRIQYEEPLVESRYLYDPLGRRTGKRVWRRERDLTGWM
                     SLSRKPEVTWYGWDGDRLTTVQTDTTRIQTVYQPGSFAPLIRIETDNGEREKAQRRSL
                     AEKLQQEGSEDGHGVVFPAELVRLLDRLEEEIRADRVSSESRAWLAQCGLTVEQLARQ
                     VEPEYTPARKAHLYHCDHRGLPLALISEDGNTAWSAEYDEWGNQLNEENPHHVYQPYR
                     LPGQQHDEESGLYYNRHRYYDPLQGRYITQDPMGLKGGWNLYQYPLNPLQQIDPMGLL
                     QTWDDARSGACTGGVCGVLSRIIGPSKFDSTADAALDALKETQNRSLCNDMEYSGIVC
                     KDTNGKYFASKAETDNLRKESYPLKRKCPTGTDRVAAYHTH"
BASE COUNT          926 a         1152 c         1384 g          680 t
ORIGIN      
        1 attttatttt ttttgttgct aacacaggga tatgaacaat aactaaaagg gcactttata
       61 tgagcggaaa accagcggcg cgtcagggag atatgactca gtatggcggt cccattgtcc
      121 agggttcggc aggtgtaaga attggcgcgc ccaccggcgt ggcgtgctcg gtgtgtccgg
      181 gcgggatgac ttcgggcaac ccggtaaatc cgctgctggg ggcgaaggtg ctgcccggcg
      241 agacggacct tgcgctgccc ggcccgctgc cgttcattct ctcccgcacc tacagcagct
      301 accggacgaa gacgcctgca ccggtgggcg ttttcggccc cggctggaaa gcgccttctg
      361 atatccgctt acagctacgt gatgacggac tgatactcaa cgacaacggc gggcggagca
      421 ttcactttga gccgctgctg ccgggggagg cggtgtacag ccgcagtgag tcaatgtggc
      481 tggtgcgcgg tggtaaggca gcacagccgg acggccatac gctggcgcgg ctgtgggggg
      541 cgctgccgcc ggatatccgg ttaagcccgc atctttacct ggcgaccaac agcgcacagg
      601 ggccgtggtg gatactgggg tggtcagagc tggtgccggg tgctgaggac gtactgccag
      661 cgccgctgcc gccgtaccgg gtgcttaccg ggctggcaga ccgcttcggg cggacgctga
      721 cgtaccggcg tgaggccgcc ggtgacctgg ccggggaaat caccggcgtg acggacggtg
      781 ccgggcggga gttccgtctg gtgctgacca cgcaggcgca gcgtgcggaa gaggcccgta
      841 aacagcgcac cgcttcctta tcttcccctg acacaccccg ccctctctca gcctcagcgt
      901 tccccgacac actgcccggt accgaatacg gccccgacag gggcatccgt ctttcggcgg
      961 tgtggctgat gcacgacccg gcatacccgg aaagcctgcc cggtgcgcca ctggcgcggt
     1021 acacgtatac ggaagccggt gaactgctgg cggtatatga ccgcagcaat acgcaggtgc
     1081 gcgctttcac gtatgacgcg cagcacccgg gccggatggt ggcgcaccgt tacgcgggaa
     1141 ggccggagat gcgctaccgc tacgacgata cggggcgggt ggtggagcaa ctgaacccgg
     1201 cagggttaag ctaccgctat ctttatgagc aggaccgcat caccgtcacc gacagcctga
     1261 accggcgtga ggtgctgcat acagagggcg gggccgggct gaaacgggtg gtgaaaaaag
     1321 aactggcgga cggcagcgtc acgcgcagcg ggtatgacgc ggcaggaagg ctcacggcgc
     1381 agacggacgc ggcgggacgg cggacagagt acggtctgaa tgtggtgtcc ggcgatatca
     1441 cggacatcac cacaccggac gggcgggaga cgaaatttta ctataacgac gggaaccagc
     1501 tgacggcggt ggtgtccccg gacgggctgg agagccgccg ggaatatgat gaaccgggca
     1561 ggctggtatc ggagacatcg cgcagcgggg agacagtacg ctaccgctac gatgacgcgc
     1621 acagtgagtt accggcgacg acaacagatg cgacgggcag cacccggcag atgacctgga
     1681 gccgctacgg gcagttgctg gcgttcaccg actgctcggg ctaccagacc cgttatgaat
     1741 acgaccgctt cggccagatg acggcggtcc accgcgagga aggcatcagc ctttaccgcc
     1801 gctatgacaa ccgtggccgg ttaacctcgg tgaaagacgc acagggccgt gaaacgcggt
     1861 atgaatacaa cgccgcaggc gacctgactg ccgttatcac cccggacggc aaccggagcg
     1921 agacacagta cgatgcgtgg ggaaaggcgg tcagcaccac gcagggcggg ctgacgcgca
     1981 gtatggagta cgatgctgcc ggacgtgtca tcagcctgac caacgagaac ggcagccaca
     2041 gcgtcttcag ttacgatgcg ctggaccggc tggtacagca gggcggcttt gacgggcgga
     2101 cgcaacgtta tcattatgac ctgaccggaa aactcacgca gagtgaagat gaggggcttg
     2161 tcaccctctg gcactacgat gcgtcggacc gcatcacgca ccggacggtg aacggcgacc
     2221 cggcagagca gtggcagtat gacggccacg gctggctgag agacatcagc cacctgagcg
     2281 aaggccaccg tgtcgctgtc cactacggct acgacgataa gggccgcctg acgggcgaac
     2341 ggcagacggt ggagaacccg gagacggggg aactgctgtg gcagcatgag acgaaacacg
     2401 catacaacga acaggggctg gcaaaccgcg tcacgccgga cagcctgccg ccggtggagt
     2461 ggctgaccta cggcagcggt tacctggcgg gcatgaagct gggcgggacg ccgctggtcg
     2521 agtatacgcg ggacaggctg caccgggaga cggtgcgcag cttcggcagc agggcaggca
     2581 gtaatgccgc atatgaactg accagcacat acacccccgc aggccagtta cagagccagc
     2641 acctgaacag cctggtgtat gaccgtgact acgggtggaa tgacaacggc gacctggtgc
     2701 gcatcagcgg cccgcgacag acgcgggaat acggctacag tgccacgggc aggctggaga
     2761 gcgtgcgcac cctcgcaccg gacctggaca tccgcatccc gtatgccacg gacccggcgg
     2821 gcaaccggct gccggacccg gagctgcacc cggacagtac cctcacagtg tggccggata
     2881 accgcatcgc gaaggatgcg cactatgttt accactacga tgaatacggc agactgacgg
     2941 agaagacgga ccgcatcccg gcgggtgtga tacggacgga cgacgagcgc acccatcact
     3001 accattacga cagcctgcac cgcctggtcc attacatccg tatccaatat gaggagccgc
     3061 tggtcgagag ccgatacctt tacgacccgc tgggtcgccg gacagggaaa cgggtgtggc
     3121 gtcgcgagcg tgacctgacg ggatggatgt cgctgtcgcg gaaaccggag gtgacgtggt
     3181 acgggtggga cggcgaccgg ctgacgacgg tacagaccga caccacgcgt atccagacgg
     3241 tatatcagcc ggggagcttc gcgccgctca tccgcatcga aacagacaac ggcgagcggg
     3301 agaaagcgca gcgccgcagc ctggcggaga agctgcagca ggaagggagc gaggacgggc
     3361 acggcgtggt atttccggct gaactggtgc ggctgctgga cagactggag gaagaaatcc
     3421 gggcagaccg cgtgagcagt gaaagccggg cgtggcttgc gcagtgcggg ctgacggtgg
     3481 agcaactggc cagacaggtg gagccggaat acacaccggc gcgaaaagct catctttatc
     3541 actgcgacca ccggggactg ccgctggcgc ttatcagcga agacggcaat acggcgtgga
     3601 gcgcggaata tgatgaatgg ggcaaccagc ttaatgagga gaacccgcat catgtgtatc
     3661 agccgtaccg tctgccaggg cagcagcatg atgaggaatc agggctgtac tataaccgtc
     3721 accggtacta cgatccgttg caggggcggt atattactca ggacccgatg gggttgaaag
     3781 ggggatggaa tttatatcag tatcctttaa atccactaca acaaattgac cctatgggat
     3841 tattgcagac ttgggatgat gccagatctg gagcatgtac ggggggagtt tgtggtgttc
     3901 tttcacgtat aataggacca agtaaatttg atagtactgc agatgctgcg ttagatgctt
     3961 tgaaagaaac gcagaataga tctctatgta atgatatgga atactctggt attgtctgta
     4021 aagatactaa tggaaaatat tttgcatcta aggcagaaac tgataattta agaaaggagt
     4081 catatcctct gaaaagaaaa tgtcccacag gtacagatag agttgctgct tatcatactc
     4141 ac
//
LOCUS       DAPHDW010000234         4139 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.239, whole genome shotgun sequence.
ACCESSION   DAPHDW010000234 DAPHDW010000000
VERSION     DAPHDW010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.239"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            227..835
                     /locus_tag="RH057_004371"
     CDS             227..835
                     /locus_tag="RH057_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HDV3130628.1"
                     /translation="MKLVGSYTSPFVRKLSILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            933..2324
                     /gene="selA"
                     /locus_tag="RH057_004372"
     CDS             933..2324
                     /gene="selA"
                     /locus_tag="RH057_004372"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HDV3130629.1"
                     /translation="MTTEMRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLAAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            2321..>4139
                     /gene="selB"
                     /locus_tag="RH057_004373"
     CDS             2321..>4139
                     /gene="selB"
                     /locus_tag="RH057_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HDV3130630.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVNEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPGREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETAHAGQRIALNIAGDAEKEQINRGDWLLADAPPEPFTRVIVELQTHT
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQSDADALSVHLERGAVNLADFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLR"
BASE COUNT          929 a         1047 c         1220 g          943 t
ORIGIN      
        1 gctcatcctt gttttatcgg gttatttgtc tgcttccagg caaaacccta cagatttata
       61 agaaagcctt aagtgagata tccatcagat agaaacaatt aggaaaatat ttatatcacc
      121 atgataaata tgtgatttat ttggaagtaa caaaccatca acattttctg ccctttttcc
      181 catcaccagg ccatactgat atttttcctc gcacaaagga ctcggcatga aactcgtcgg
      241 tagctacacc agcccgtttg tacgcaaact ttctattctg ttgttagaaa agggcataac
      301 tttcgaattt attaatgaac tgccctataa cgcggacaac ggcgtggcgc aatttaaccc
      361 gttaggaaaa gtgccggtgc tggtgaccga agagggcgaa tgttggtttg attcgccgat
      421 catcgctgaa tatattgaat taatgaatgt cgctccggcg atgttgccgc gcgatccgct
      481 ggagtcgttg cgggtgcgca aaattgaggc actggcggat ggcattatgg atgccgggct
      541 ggtatcggtg cgtgaacagg cgcgtccagc ggcgcagcag tctgaagatg aattgttacg
      601 ccagcgggag aaaatcaacc gcagtctgga tgtgctggaa ggatatctgg tcgatggcac
      661 actcaaaacc gatacggtca atctggcgac tatcgccatt gcctgtgccg tcggatatct
      721 caatttccgc cgcgttgcgc caggctggtg tgtcgatcgc ccacacttag tcaaactggt
      781 cgaaaacctg tttagccgcg aaagttttgc tcgcaccgaa ccgccaaagg cttgatgcgc
      841 gatatgtcct cctgacccat ctcacgttac aatccgtggt tatgttaaac gcccttctcc
      901 gtgtgagagg gccttgatca gccaggtttc ctatgacaac cgaaatgcgt tccctctata
      961 gtcaacttcc ggctattgat cgcttattgc gcgatagctc cttcctttct ttgcgtgata
     1021 cttatggtca cacccgcgtg gtggaattgt tgcgtcagat gctcgacgaa gcgcgagaag
     1081 tgattcgtgg cagccagacg ctgcctgcgt ggtgtgaaaa ctgggcgcaa gaagtcgatg
     1141 cccggttgac gaaagaagcg cagagcgcgc tgcgtccggt gatcaacctg acgggaaccg
     1201 tgctgcatac caaccttggg cgagctttac aggcggaagc cgcggtggaa gccgttgcgc
     1261 aggctatgcg ttcgccagtg accctcgagt acgatctgga cgacgctgga cgcggacatc
     1321 gcgatcgtgc gctggcgcag ttgctgtgcc gcattacggg ggcggaagat gcctgtatcg
     1381 ttaataacaa tgcggcggcg gtgttattga tgttggcggc cactgccagc gggaaagagg
     1441 tggtggtatc tcgcggcgaa ctggtggaga ttggcggcgc gtttcgtatt cccgatgtta
     1501 tgcgtcaggc aggctgcacc ctacacgaag tagggaccac caaccgcacg cacgcgaatg
     1561 attatcgtca ggcggtgaat gaaaataccg cactgttgat gaaagtacat accagtaact
     1621 acagcattca ggggttcacc aaagcgatag atgaagcgga actggtggcg ctcggcaaag
     1681 agctggatgt tcccgtagtg actgatttag gcagtggctc gctggtcgat cttagccagt
     1741 acggtttgcc gaaagagcca atgccgcagg agttgattgc ggcgggcgtc agtctggtga
     1801 gtttctccgg cgacaagttg ttaggcgggc cgcaggcagg aattattgtt ggtaaaaaag
     1861 agatgatcgc ccgcctgcaa agccacccgc tgaagcgtgc attacgcgcg gataaaatga
     1921 ccctcgcggc gctggaagcc acgttgcgtc tttatttaca ccctgaagct ctgagtgaaa
     1981 aattaccgac cctgcgcctg cttacccgca gcgcagaggt cattcaaatc caggcacaac
     2041 gtttacaggc cccccttgcc gcacattacg gcgcggagtt tgcggtacag gttatgccat
     2101 gtctttcgca gattggcagt ggttcgctgc cggttgatcg cctgccgagc gcggcattaa
     2161 cgtttacacc ccatgatgga cgcggtagcc accttgagtc attagccgcc cgctggcgtg
     2221 aattgccagt gccggtgatt ggtcgtattt atgacggacg attgtggctg gatttacgct
     2281 gccttgaaga tgagcaacgg tttttggaga tgttgttgaa atgattattg cgactgccgg
     2341 acacgttgac cacggcaaaa caaccttatt gcaggcgatt actggcgtaa atgctgaccg
     2401 tctgccggaa gaaaaaaagc gcggcatgac catcgatctc ggctatgcct actggccgca
     2461 gccggatggt cgcgtgcctg gttttatcga cgtacccggt catgaaaagt ttctttccaa
     2521 catgctggcg ggcgttggtg gtatcgatca cgcgctgttg gtggtggcgt gcgatgacgg
     2581 cgtgatggca cagacccgtg agcatctggc gattttgcag ctgaccggta acccgatgct
     2641 gacagtggcg ctgaccaaag ccgatcgcgt ggacgaagcg cgtgttaatg aggttgaacg
     2701 ccaggtaaag gaggtgctgc gggaatacgg ttttgctgag gcaaaactgt ttatcaccgc
     2761 agcaaccgaa ggtcggggaa ttgatgccct gcgcgagcat ctgcttcagt taccaggacg
     2821 tgaacacgcc agccaacata gtttccgcct cgcgatcgac cgcgcattta ccgtaaaagg
     2881 tgccgggctg gtcgtcaccg gtacggcgtt aagcggggaa gtgaaggtag gcgattcact
     2941 ctggctgact ggtgtaaata aaccgatgcg tgtacgtgcg ctgcatgcac aaaaccagcc
     3001 aacagaaacc gcccatgccg ggcagcgtat cgcgcttaac atcgcgggtg atgcggaaaa
     3061 agagcagatt aaccgtggcg actggctgct tgccgatgcg ccgccagagc cgttcacacg
     3121 ggtgattgtc gagcttcaaa cccatacgcc gctgacccag tggcagccgc tgcatattca
     3181 ccacgccgcc agccacgtta cgggacgcgt ttcgctgctg gaagataacc ttgccgagct
     3241 ggtgttcgac acgccgctgt ggctggcgga taacgatcgc ttagtgctgc gcgatatctc
     3301 ggcccgcaac acgctggcgg gggcgcgtgt ggtgatgctt aacccgccgc gtcgcggtaa
     3361 acgtaagccg gaatatctgc aatggctggc gtctcttgca cgggcgcaga gcgatgccga
     3421 tgcgttatct gttcatctgg aacgcggcgc ggttaacctt gcggatttcg cctgggcgcg
     3481 ccagctcaac ggcgaaggga tgcgcgaatt gctgcaacag cctggctata ttcaggctgg
     3541 ttatagcttg ttgaatgcgc cggttgccgc tcgctggcaa cggaaaattc tcgacacatt
     3601 agcgacttat catgagcaac atcgcgatga acctgggcct gggcgcgaac gtctgcgacg
     3661 tatggcgttg ccaatggaag atgaagcgct agtactgttg ctgattgaaa agatgcgcga
     3721 aagcggcgac atcctcagtc atcacggttg gctgcatctg ccggatcaca aagcgggctt
     3781 cagcgaagag cagcaggcca tctggcaaaa agcagagccg ctgtttggtg acgagccgtg
     3841 gtgggtgcgt gatctggcaa aagagacggg taccgacgag caggcaatgc gcctgacgct
     3901 acgccaggcg gcgcagcaag gaataattac cgcgatcgtc aaagatcgtt attaccgtaa
     3961 cgatcggatt gtcgaatttg ccaatatgat ccgcgatctc gatcaggagt gtggttcaac
     4021 ctgcgcggcg gatttccgcg atcgcttagg cgtaggccga aagctggcaa ttcagattct
     4081 ggaatatttt gaccgcattg gctttacgcg tcgtcgtgga aatgatcatt tattgcgcg
//
LOCUS       DAPHDW010000235         3951 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.240, whole genome shotgun sequence.
ACCESSION   DAPHDW010000235 DAPHDW010000000
VERSION     DAPHDW010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3951
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.240"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..1713
                     /gene="dpiB"
                     /locus_tag="RH057_004374"
     CDS             55..1713
                     /gene="dpiB"
                     /locus_tag="RH057_004374"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase DpiB"
                     /protein_id="HDV3130631.1"
                     /translation="MLQLNENKQFAFFQRLAFPLRIFLLILVFSIFVIAALAQYFTAS
                     FEDYLTLHVRDMAMNQAKIIASNDSVISAVKTRDYKRLATIANKLQRDTDFDYVVIGD
                     RHSIRLYHPNPEKIGYPMQFTKQGALEKGESYFITGKGSMGMAMRAKTPIFDDDGKVI
                     GVVSIGYLVSKIDSWRAEFLLPMAGVFVVLLGILMLLSWFLAAHIRRQMMGMEPKQIA
                     RVVRQQEALFSSVYEGLIAVDPHGYITAINRNARKMLGLSSPGRQWLGKPIAEVVRPA
                     DFFTEQIDEKRQDVVANFNGLSVIANREAIRSGDDLLGAIISFRSKDEISTLNAQLTQ
                     IKQYVESLRTLRHEHLNWMSTLNGLLQMKEYDRVLAMVQGESQAQQQLIDSLREAFAD
                     RQVAGLLFGKVQRARELGLKMIIVPGSQLSQLPPGLDSTEFAAIVGNLLDNAFEASLR
                     SDEGNKIVELFLSDEGDDVVIEVADQGCGVPESLRDKIFEQGVSTRADEPGEHGIGLY
                     LIASYVTRCGGVITLEDNDPCGTLFSIYIPKVKPNDSSINPIDR"
     gene            1682..2362
                     /gene="dpiA"
                     /locus_tag="RH057_004375"
                     /gene_synonym="citB"
     CDS             1682..2362
                     /gene="dpiA"
                     /locus_tag="RH057_004375"
                     /gene_synonym="citB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component response regulator DpiA"
                     /protein_id="HDV3130632.1"
                     /translation="MTAPLTLLIVEDETPLAEMHAEYIRHIPGFSQILLAGNLAQARM
                     MIERFKPGLILLDNYLPDGRGINLLHELVQAHYPGDVVFTTAASDMETVSEAVRCGVF
                     DYLIKPIAYERLGQTLTRFRQRKHMLESIDSASQKQIDEMFNAYARGEPKDELPTGID
                     PLTLNAVRKLFKEPGVQHTAETVAQALTISRTTARRYLEYCASRHLIIAEIVHGKVGR
                     PQRIYHSG"
     gene            complement(2403..3788)
                     /gene="dcuC"
                     /locus_tag="RH057_004376"
     CDS             complement(2403..3788)
                     /gene="dcuC"
                     /locus_tag="RH057_004376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HDV3130633.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
BASE COUNT         1084 a          940 c         1018 g          909 t
ORIGIN      
        1 caacatttta attacatccg tcaaagaggc tcgggacaac ccgtaaggaa aacaatgttg
       61 cagcttaacg agaataaaca gtttgcattt ttccaaagac tggcatttcc gctgcgtatc
      121 tttttgctga ttctggtgtt ctcaatattt gtcattgcag ccctggcgca atattttacg
      181 gccagttttg aggactattt aacgcttcat gtacgcgaca tggcaatgaa tcaggcgaaa
      241 attattgcct ccaatgacag tgtcatctct gcggtgaaaa cgcgtgacta caaacggctg
      301 gcgaccatcg ctaacaaatt acaaagagat accgattttg attatgtggt gattggggac
      361 cggcactcga tccgccttta ccatcctaat ccggagaaaa ttggttatcc tatgcagttc
      421 accaaacagg gcgcgctgga gaaaggggag agctacttca ttaccgggaa agggtcaatg
      481 gggatggcga tgcgcgccaa aacgccaatc tttgatgacg atggaaaagt catcggcgtg
      541 gtgtcgattg gctacctggt gagtaaaatc gatagctggc gggctgagtt tttattaccg
      601 atggcaggcg tgtttgtcgt gctgttaggg attctgatgt tgctgtcgtg gttcctggca
      661 gcgcatatcc gtcggcaaat gatgggcatg gagccaaagc aaatcgcacg cgtggtccgt
      721 cagcaagagg cgctgtttag ttcggtttat gaagggctga ttgcggtgga tccgcatggt
      781 tacattaccg ccatcaatcg taacgcaaga aagatgctgg ggctgagctc tcccggacgg
      841 caatggttgg gtaaacccat tgctgaagtg gtcaggcccg ccgatttctt taccgaacag
      901 attgatgaaa aacgtcagga tgtggtggct aactttaacg gtctgagcgt tattgccaat
      961 cgggaagcta ttcgttcagg tgatgatttg ctgggggcca ttatcagctt tcgtagtaaa
     1021 gacgaaattt ccaccctcaa tgcgcaactg acgcaaataa aacaatacgt cgagagcctt
     1081 cgtacattgc gacacgagca tctcaattgg atgtcgacgc tcaatggtct gttgcagatg
     1141 aaagagtatg atcgcgtgct ggcgatggtg cagggggagt ctcaggccca gcaacagctt
     1201 attgacagcc tgcgtgaggc gtttgccgat cgccaggtgg cggggctgct ttttggtaaa
     1261 gtgcagcgcg cccgggaact ggggctaaaa atgatcattg tccccggtag ccagctttcg
     1321 caactgccgc caggactgga tagcaccgag tttgcagcca ttgtgggcaa tttacttgat
     1381 aacgccttcg aagccagcct gcgtagcgat gaaggaaaca agatcgttga attattcctc
     1441 agcgatgaag gcgatgatgt ggtgattgaa gtcgccgatc agggctgcgg cgttccagag
     1501 tctctacgag acaaaatatt tgagcagggg gtcagtacgc gtgctgacga gcccggtgaa
     1561 catggcattg ggttgtactt gattgccagc tacgtaacgc gctgcggtgg tgttatcact
     1621 ctcgaagata atgatccctg cggtaccttg ttttcaatct atattccgaa agtgaaacct
     1681 aatgacagct ccattaaccc tattgatcgt tgaggacgaa acgccgctgg cagagatgca
     1741 tgcggaatat attcgtcaca ttcccggatt cagtcagata ttactggcgg gaaacctggc
     1801 gcaggcccga atgatgatcg agcgttttaa gccggggcta atcttgctcg ataactatct
     1861 tcctgacggc agagggatta atttattgca tgaactggtg caggcgcatt atcccggcga
     1921 cgtggtgttt accactgcag ccagcgatat ggaaacggtg tctgaagccg tacgttgtgg
     1981 tgtatttgat tatctcatta agcccattgc ctatgaacgg ctggggcaaa cgctaacccg
     2041 tttccgccag cgtaaacata tgctggaaag tattgatagc gccagccaga agcaaattga
     2101 tgagatgttt aatgcctatg ctcgcggtga acctaaagac gaactgccga ccggcattga
     2161 tcccttgacg ctaaacgcgg tgcgaaaact gtttaaagag cctggtgtgc aacatacggc
     2221 agaaacggtg gcgcaggcac tgaccatcag tcgcaccact gccaggcgtt atcttgaata
     2281 ttgcgccagc cgccatctga ttattgctga aattgttcac ggcaaagttg gcagaccgca
     2341 acgcatatac cacagtgggt gacatgataa agccggagga aacttgcctc cggcattact
     2401 atttacttgc ctgtgaccgc tgctgccgtt cctggcacca tcagctctgt agcaacgata
     2461 acaatcacca aaccaacaag caccggtacc gaggtgcgtt ttacgacttc aaacggcgag
     2521 atcttcgcca tcccagcaac cgcaacgact acgccagaaa cgggcgaaag ggtacggccc
     2581 aggtttgacg cctgcagcat cgggatagtc aaatacgccg ggttaatgcc ggaagagtgc
     2641 gccagtttcg ggatcatctc aacaaacgca taaaacggcg cattgcctga accggtcgtg
     2701 actgccgcca gcatggtcag aatcaccaat accagcatca ggatgatact cgccgaacca
     2761 aacgaggtag cgatagaaat cagactttga ataaagccga tggtgctaag cccctgagcg
     2821 aataccccag cggcaaccag cagcatcacc acgttagcaa acgcatctgc cattccgcga
     2881 taagccactt ccagaccaga gaaaactttc tgggtattaa agctgcggag gaactccaga
     2941 atggaggcaa tcagcataca aatcaccaga atagtgatga tgtgtaattg cggaccccat
     3001 ttaccgtcaa aaatcagtac accgatgatc ggcgtgaacg gcaaaatggc ataaaacgca
     3061 ggagcagtgg tggtgatttc actgacatct aacatttcat gagagatgtg ctctttttta
     3121 tccagataac gttgccagaa gaagtgggcg atcgccatgc cgataattgc agcaattgag
     3181 ataggcagcg tcgttttgaa ggcgaagtca atcagcgaca tttcggaagc ttgcgccgcc
     3241 agcaccacat cccctgaagt cggtgcgaga ataatcgccg ccggggaggc acaaatggca
     3301 gcagctgcgc cacgactgat accaacgttt accatcaccg gaaatagggt tgccatcagc
     3361 aaaacaccca gaccggttgc ggaagagacg gccagagaca tcagacaggc gacaaaatag
     3421 gcggcaatca tcagcaggta aggggagtta atatactgca atggttttga cgccagcttg
     3481 accaccatat cattcgcgcc gatatgggtc atgtaagcgg caaatccaca cagcatcata
     3541 atcatcatgc cgaggtcgcc gccgcggctc attagtaata ttttaacgta ttcaacgata
     3601 tccgtggcgc tgtagcctgt tgaagcctgg ctggacggta acactttgtg ccccataatg
     3661 gcactgataa tcagcaataa caggccaccg acaaataaca caccagtggc ggaataccct
     3721 ttaatgatgt agcgagctac acccacaata accacaaccc caataaggag ttcaatgaat
     3781 gtcagcataa tttttcctgt ctccaggccc caaagtaaat aataaaaaat tcctaaagct
     3841 taaggaaaaa atatgcccaa taaattggcg atgaatgctg attaaaatca agaaaaactg
     3901 ccattaagac attgaagttg ctgtttttat acatagataa caaaacgcat a
//
LOCUS       DAPHDW010000236         3866 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.241, whole genome shotgun sequence.
ACCESSION   DAPHDW010000236 DAPHDW010000000
VERSION     DAPHDW010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3866
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.241"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(97..2577)
                     /locus_tag="RH057_004377"
     CDS             complement(97..2577)
                     /locus_tag="RH057_004377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HDV3130634.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSIEVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYTWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNSKSTYVRFNSGLNLLGWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQLLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGRSHIEGASKQSDFVQAGYQYGFNNLLTLYGGSMVANNYYAFTLGTGW
                     NTRIGAISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRT
                     FNDHVWANNKDNYRRDENDVYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLW
                     RDYWGRSGSSKDYQLSYSNNWRRISYILAASQAYDENHHEEKRFNIFISIPFDWGDDV
                     TTPRRQIYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSYQNQGNETTAGANL
                     TWNAPVATVNGSYSQSSTYRQAGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDA
                     YVNGQKYRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVL
                     VNFDTDQRKPWFIKALRADGQPLMFGYEVNDIHGHNIGVVGQGSQLFIRTNEVPPSVN
                     VAIDKQQGLSCTITFGKEIDESRNYICQ"
     gene            complement(2593..3267)
                     /locus_tag="RH057_004378"
     CDS             complement(2593..3267)
                     /locus_tag="RH057_004378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HDV3130635.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLDIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVSRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNVKSNNANNWYLTIIDDHGN
                     YISDKI"
     gene            complement(3348..>3866)
                     /locus_tag="RH057_004379"
     CDS             complement(3348..>3866)
                     /locus_tag="RH057_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3130636.1"
                     /translation="AVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFEAGGDSVSI
                     NMPTVPTTVFEGKAKYSTYDDAVGVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAI
                     ASSNDTVGYYLYLGDNSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGD
                     VLSSLVMRVAQD"
BASE COUNT         1080 a          962 c          715 g         1109 t
ORIGIN      
        1 acagataact cactataaca tgccgcatag ctcacaggca taaccatcat cattactata
       61 aataacatta tacgaatatt cataaccacc tgactattac tggcaaatat agtttctact
      121 ttcatcaatc tctttaccga aggtgattgt gcatgaaagt ccttgttgct tatcaattgc
      181 tacattaacc gatggcggta cttcattggt gcgaataaat aactggcttc cctgaccgac
      241 aacgccaata ttatgaccat ggatatcatt gacttcataa ccaaacatta atggttgccc
      301 atccgctctt aacgctttta taaaccaggg cttgcgctga tcggtatcaa aattaaccag
      361 tacaaccgcg ccgcgataag gggcggcaat tttccggttg ccacgtaatt ctgcttcgct
      421 atcgctttgc gacacatcca gcatcaggtg attttcccga taaggtgtca ttccgtcgta
      481 taccaccact ccattacggt ttgttgtgcg atatttttgc ccattgacat aagcatcttt
      541 aattcctggc gcattcatca cagcaaacgt ttcggaaaga cggttcgcca gattaacgcc
      601 ccccgaccag gcgacaatgc cccctgaaac actggctcca gcctgtcgat aagtactcga
      661 ctgactataa ctgccattca ctgtcgcaac cggcgcgttc caggtcaaat tcgccccagc
      721 tgtcgtttca tttccctgat tctgataact caggttgacc ccatagttaa actgatcccg
      781 gcttccaacg gttcctgata atcccgtatt atttgaggca aacccctgat catcaaacgt
      841 cgttgagtta gacatatata tttgccgacg aggcgtcgta acgtcatcac cccaatcaaa
      901 gggaatcgat ataaaaatat taaaacgttt ctcttcatga tgattctcgt catacgcctg
      961 gcttgccgcg aggatatagc ttatccgccg ccagttattg gaataactca actgataatc
     1021 cttactgctg ccgctacgcc cccagtaatc tcgccataac gtacttaagg acacagaccc
     1081 ccagccttct ggcaatgact ggctcatatt ggcagagaag ctatttttac ggccaaaatc
     1141 gttctggtaa taatcggcaa tgtcatagac atcgttttca tcacggcgat aattatcttt
     1201 attgtttgcc caaacgtgat cattaaatgt ccgataatca cgcgatgaat aacgccaggc
     1261 cgccagacca aaacgcgtcg acgtttggct cacaaatttg ttgtaggcaa tttgataact
     1321 ttgcccgtca aacacatcgc cgttgtcttg tttactatgc gacttcgtgg catcgacgga
     1381 aatggcacca atgcgtgtat tccagccagt ccccaaagta aacgcgtaat aattattcgc
     1441 gaccatcgag ccaccataca gcgtcaataa attattaaaa ccatactgat aacccgcctg
     1501 gacaaaatca ctttgtttgc tcgccccttc aatatggcta cgacccgccg caaaatcata
     1561 tttcgatacg ccgggttgca gcatatttgg cactgctgca taaggcacca gataggtggt
     1621 taccgagccg tccgcctctt tcacgctgac atcaagatct gctccaccac cggccaactg
     1681 caaatctgta atcgcgaacg ggccaggagg aacctctttc tgataaacca caaaaccgtt
     1741 ctgttcaata gttaccagcg cattactctg agcaatcccc tgcacccgtg gcgtaaaatt
     1801 ttgtttcgag ttaggcaaca tctgcatatc acgaaacaac cgcacaccgc tgaagcgaac
     1861 agaatcaaaa atatcgcttg atgtgtacat atcacccacg cgaagcgtgc cgagaagttg
     1921 ggcaaatcca cgttccagat acagggtatt gcttttccac acccctggat tgttatttgt
     1981 tttactgaaa ctggcatcag aatgcagttg ccaccccagt aaatttaacc cgctgttaaa
     2041 acgtacatat gtactcttac tattacccga cgctttatag tcactgtaat actgactcac
     2101 ataataagag gtataaaacg cattaatacc ccgctcccag ttttccggtg gaacatagcc
     2161 actttccagt tcttccaccc aggcctgcgg gacactgaaa tcgagacgaa aaaccccgat
     2221 atcccaggta tagctcccac cctgaacaag ttgctcaaat gttaaacatt gcttaccgct
     2281 ggcgaagtta tcgctattaa tgcctaaccg cttgataact tctattgata aacatgtttc
     2341 ttgcgggttg tctttaacaa taatctcata tttcccgcgc cattgcttat tgacataaat
     2401 atcgatgtca tactgccccg gtaagggttg attatcatca agacgaatat ttgcaacctg
     2461 ctggtctttc attccaccta tcataaaatg ggtatcaaag gtttcttcag ctgcataagc
     2521 ttcaatgccg agcaataacg ctacgattgc tgatgcaagt ggggtcattc tcaacatagg
     2581 cagctcctgc aattaaattt tgtcactaat atagttgccg tggtcatcga taatggtcag
     2641 ataccagtta tttgcattat tacttttgac attaacactc tgactttcta agggggcaat
     2701 cattatagtt tcataattga ctttgacatt attagctttg acatccgaaa tcgtcaccca
     2761 attagctgag tcatttttta tcaccaatcc attgccactg cgacttacca gtaacttttt
     2821 aaaggtcgct ttatttaccg gagcaatacc cgccgggcgg taaaacaatt tgattctgtt
     2881 ttgcatcgca aacttcagtg cattcttacc ttcttgttct ggactatttg gtggaatatc
     2941 aagaacattc agataaaaaa tgctttcttt attagtgggc agtttattcg gcataatttt
     3001 gatttttact tgctgcccgg aatttgcccc tatttttgcc actggtggcg ttaacatgaa
     3061 aggaacctga attttttctg gtggtaacga cgtatcgcca tcatcaatcc acgcctgcag
     3121 cagcgaagaa cggtttccct ggttcatcaa ctgcaccata acttctttat tttctgccgg
     3181 gtaaataatg cgcgtcccgt agataacaat tccggcatgt gcaggaagga ccatagaaaa
     3241 aatgagtaaa gataataatc ctttcatctt aatgcctctt aaattaaaaa gccgccatgg
     3301 ggatagcggc catgtttatt acctggagaa acttgcaatt ttatttatta atcctgcgcc
     3361 acacgcataa ccagagaaga taacacatca cctgattgca ctgagttatc tgtcagtttc
     3421 aggtattttg ctttaaacgg aatagcatat tgaccttccg ctgttttata actctcaatg
     3481 ttaaaaggtg cagaaacatc caggacatcg ctgttatcac ctaaatagag atagtaaccc
     3541 acggtatcgt tgctactggc tatcgcctta tcgttaccgg ttattttatc gttggtaatc
     3601 aacgagagtt ttacaccagc aacttctttc gggcagctaa tttttaacat gctgctggtt
     3661 acaccgactg catcatcata ggtagaatat ttagctttac cttcaaagac agtggttggt
     3721 acagtcggca tattaatact tactgaatca ccacccgctt cgaatttaca cggagattct
     3781 gcaatattcc ccttaatagt taatgttccg gtatcaacgt ctgcagcaaa tactccagcg
     3841 ctcataaaaa aagaagaaaa gacagc
//
LOCUS       DAPHDW010000237         3843 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.242, whole genome shotgun sequence.
ACCESSION   DAPHDW010000237 DAPHDW010000000
VERSION     DAPHDW010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.242"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..989)
                     /locus_tag="RH057_004380"
     CDS             complement(<1..989)
                     /locus_tag="RH057_004380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130637.1"
                     /translation="MNYAIDKFTGTLILAARATKYAQYVCPVCKKGVNLRKGKVIPPY
                     FAHLPGHGTSDCENFVPGNSIIVETIKTISKRYMDLRLLIPVGSNSREWSLELVLPTC
                     NLCRAKITLDVGGRSQTLDMRSMVKSRQIGAELSVKSYRIVSYSGEPDPKFVTEVERE
                     CPGLPSEGAAVFTALGRGASKGFPRAQELRCTETFAFLWRHPVAPDFPDELEIKSLAS
                     KQGWNLALVTIPEVPSVESISWLKSFTYLPVVPARTSITAIWPFLNQKTSINHVECVY
                     SDTILLSTNMAPTSSENVGPTMYAQGSSLLLSAVGVETSPAFFILNPGENDFVG"
     gene            complement(1011..2225)
                     /locus_tag="RH057_004381"
     CDS             complement(1011..2225)
                     /locus_tag="RH057_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4338 domain-containing protein"
                     /protein_id="HDV3130638.1"
                     /translation="MHKYPSIIVNINLREAKLKKKVREHLQSLGFTRSDSGALQAPGN
                     TKDVIRALHSSQRAERIFANQKFITLRAAKLIKFFASGNEVIPDKISPVLERVKSGTW
                     QGDLFRLAALTWSVPVSSGFGRRLRYLVWDESNGKLIGLIAIGDPVFNLAVRDNLIGW
                     DTHARSSRLVNLMDAYVLGALPPYNALLGGKLIACLLRSRDLYDDFAKVYGDTVGVIS
                     QKKKQARLLAITTTSSMGRSSVYNRLKLDGIQYLKSIGYTGGWGHFHIPDSLFIELRD
                     YLRDMDHAYADHYMFGNGPNWRLRTTKAALNALGFRDNLMKHGIQREVFISQLAENAT
                     SILQTGKGEPDLTSLLSAKEIAECAMARWMVPRSIRNPEYRLWKARDLFDFISNDSLN
                     FPPFDEIAKTVV"
     gene            3049..3777
                     /locus_tag="RH057_004382"
     CDS             3049..3777
                     /locus_tag="RH057_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001615471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDV3130639.1"
                     /translation="MKKIIAVSALAVAGMFSAQTLANESKTGFYMTGKAGTSVLSLSN
                     QRFVEGEGEWADRYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGKADSKY
                     NLDKDDWSRDDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTSISTW
                     DYGYGSSGRKSLSRSGSADNFAWSLGAGVRYDVAPDIALDLSYRYIDAGDSSVSYKDE
                     WGDKYKSEVDVKSHDIMLGMTYNF"
BASE COUNT         1113 a          835 c          750 g         1145 t
ORIGIN      
        1 cccacaaagt cattttctcc aggatttaga atgaagaaag caggtgatgt ttcaacacca
       61 accgctgaaa gtaataaaga ggaaccttgt gcgtacatag ttggtccaac attttctgat
      121 gatgttggtg ccatatttgt tgacaacaat attgtgtcag aataaacaca ttcgacatga
      181 ttaatacttg ttttttgatt taggaacggc caaattgctg taatagatgt tctggcagga
      241 acaacaggaa ggtatgtaaa agattttagc catgaaatac tctccacaga agggacttca
      301 ggaattgtaa caagagctaa attccatccc tgtttactag ctaaactttt tatttctaat
      361 tcatcaggaa aatctggagc aacagggtgt cgccaaagaa aggcaaatgt ttcagtacat
      421 cttaactctt gtgctcgtgg aaatcccttc gatgccccac gccctaaagc agtgaaaact
      481 gctgctccct cagaaggtaa acctgggcat tctctttcaa cttctgttac aaattttgga
      541 tctggttcac cactatatga aacaatacgg taagatttta ctgataattc agcaccaatc
      601 tggcgactct ttaccatact cctcatatca agcgtttggc ttctgcctcc tacatctaac
      661 gttatctttg ctctacataa attacaggtt ggcaacacta attctaatga ccactctcga
      721 ctattacttc cgacaggaat caataagcgc aaatccatat atcgctttga aatagtttta
      781 atagtttcga caatgataga atttccggga acaaaatttt cacagtctga cgtaccatgt
      841 ccgggcaaat gagcaaaata tgggggtata accttccctt tacggaggtt aacacctttt
      901 ttacaaactg ggcaaacata ttgagcatat ttcgttgctc gagctgctaa tatcagtgtc
      961 ccggtgaact tatcaatagc gtaattcact tactccccct tcagttaaga ttagacaact
     1021 gttttcgcta tctcgtcaaa cgggggaaag tttagcgagt cattactaat aaaatcaaat
     1081 agatctcttg ctttccaaag ccgatattct ggattgcgaa ttgatcgtgg aaccatccat
     1141 cgtgccatcg cacactcagc tatctcttta gcagaaagca aagaggttag atctggttca
     1201 cctttgcctg tttgcagaat actagttgca ttttctgcta gctgactgat aaacacttca
     1261 cgttgaattc catgcttcat caaattatct ctaaatccta gtgcatttaa agctgcctta
     1321 gttgtacgta aacgccagtt aggcccatta ccaaacatat aatgatctgc ataagcgtga
     1381 tccatatcac gtaagtaatc acgtaattca atgaacaagc tatcaggtat atgaaaatgc
     1441 ccccaaccgc ctgtatatcc aatcgatttt aaatattgaa ttccatccag ctttaaacgg
     1501 ttatataccg atgagcgccc catagacgat gttgttgtaa tagccaaaag acgtgcttgt
     1561 ttcttttttt gagatattac tccaacggta tcaccataga cctttgcaaa gtcatcataa
     1621 agatcgcggc tacgaagcag acatgcaatt aattttcctc ccagcagggc attataaggg
     1681 ggaagagcac cgaggacgta tgcatccatc aaattaacaa gccgggaact tctggcatga
     1741 gtatcccacc caatcaaatt atctcggact gcaaggttga acacagggtc accaattgcg
     1801 atcagcccta tcaattttcc gttgctttca tcccatacaa gataccggag acgccttcca
     1861 aatccgcttg aaacaggtac ggaccaagtt aatgctgcta acctaaagag atctccttgc
     1921 caggttcctg actttacacg ttcaagtacc ggtgaaatct tatccggaat gacctcattg
     1981 ccggatgcga aaaatttaat aagctttgcc gctcttagcg ttatgaactt ttggtttgca
     2041 aatatccgct cagctcgttg agaactatga agagcccgta ttacatcttt ggtatttccc
     2101 ggggcctgga gcgctccaga atcagatctt gtaaaaccca aggattgtaa atgctcacgt
     2161 accttctttt tcagtttggc ttctcgaagg ttgatattaa ctattataga gggatatttg
     2221 tgcattttta tctcacgttc caattagtat ttagtcccac taaatttcac ttagagcccc
     2281 agtgattaat cgcatggcac aagagagtcc cctccttgct ccctcgatcg ggacagcgcc
     2341 aatgatagca tttatacaag caattctggg agcttataga ttgtgtgcaa aacttttgat
     2401 agcaggagct tacaacagta ttgatgaaaa aaatcattca aatcgattta tagattttgt
     2461 taatgttatc tgttttcaat ctaataacca cgaatatagc taattatagg atgattacta
     2521 gttttactac cctatcaacc aaacgttcac cggacattag aaaacatgga aatttcttgt
     2581 ttttaaatgc tttttacacc aatagcaaaa tttacattcc aaaatcatta gtctttgcgt
     2641 aatcaaaaga tgtaatggtt ttttccttac tacttctaga taatccgccc actacagcaa
     2701 catcaactta cgctcatcta aatgctccgc cttatggata taagccaccc gcactgaatt
     2761 acattccatg cggctcatca gacgtccagt tgctttactg tcttcagggg gatgctcttg
     2821 taatcccaac ctctcgtgct ttctgagcat aacacatcca tgttgctgcg attatctctt
     2881 aaacccgtcc ttaccatttt tattgatacg ataaggaact ttcatcatac atgatcgatt
     2941 ttgtcgatcg attcgatcga attttctctt tttaccctgt cttttacatg gttttaaaat
     3001 aagcacccca tttattgttc ggggaaagtg atagcagagg taactgaaat gaaaaagatt
     3061 attgcggttt cggcacttgc tgtggcaggc atgttttcag cccagaccct ggctaatgag
     3121 agcaaaacag gcttttatat gaccggtaag gcgggaactt ccgttttatc tctttccaac
     3181 cagcgttttg tcgaaggtga aggagagtgg gcggacagat acaaaggtgg cgatgaccat
     3241 gatacggtat tcagtggcgg tattgcggcc ggttatgatt tttatccgca attcagtatt
     3301 ccggttcgta cggaactgga gttttacgcg cgtggaaaag ctgattcgaa gtataacctt
     3361 gataaagacg actggtcgcg tgatgacctg aagaatgagg tatcagtcaa cactcttatg
     3421 ctgaatgcgt actatgactt ccggaatgac agtgcattca caccatgggt atctgcaggg
     3481 attggctacg ccagaattca ccagaaaaca accagtatca gtacctggga ttatgggtac
     3541 ggaagcagtg gtcgcaaatc gttgtcacgt tcaggatctg ctgacaactt cgcatggagc
     3601 cttggcgcgg gtgtccgcta tgacgtagcc ccagatatcg ctctggacct cagctatcgc
     3661 tatattgatg cgggtgacag cagtgtgagt tacaaggacg agtggggcga taaatataaa
     3721 tcagaagttg atgttaaaag tcatgacatc atgcttggta tgacttataa cttctgacga
     3781 catttctcct gaacgataat tgcgtatatt ctgtaattaa gataattgca tatcttctgc
     3841 aat
//
LOCUS       DAPHDW010000238         3779 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.243, whole genome shotgun sequence.
ACCESSION   DAPHDW010000238 DAPHDW010000000
VERSION     DAPHDW010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3779
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.243"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(14..1222)
                     /locus_tag="RH057_004383"
     CDS             complement(14..1222)
                     /locus_tag="RH057_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HDV3130640.1"
                     /translation="MDEKQLQALANELAKNLKTPEDLSQFDRLLKKLSVEAALNAEMT
                     HHLGYEKNQSRPGANSRNGFSTKTVITGDGPLELRTPRDRDGTFEPQLVKKNQTRITG
                     MDNQILSLYAKGMTTREIAAAFKELYDADVSPALISKVTDAVMEQVVEWQNRPLDAVY
                     PIVYLDCIVLKVRQDSRVINKSVFLALGINIEGQKELLGMWLAENEGAKFWLNVLTEL
                     KNRGLNDILIACVDGLKGFPDAINTVYPKARIQLCIVHMVRNSLRFVSWKDYKAVTRD
                     LKAIYQAPTEEAGQQALEAFAAAWDCRYPQISRSWQANWPNLATFFAYPTDIRKVIYT
                     TNAIESLNSVIRHAIKKRKVFPTDDSVKKVVWLAIQAASQKWTMPLRDWRMAMSRFII
                     EFGDRLDGHF"
     gene            1283..1432
                     /locus_tag="RH057_004384"
     CDS             1283..1432
                     /locus_tag="RH057_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002208622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130641.1"
                     /translation="MYRLISHTRYKLTSAELEAFNSVVDHWLAELTMNKLYDRVPASV
                     WKYVI"
     gene            complement(1847..2047)
                     /locus_tag="RH057_004385"
     CDS             complement(1847..2047)
                     /locus_tag="RH057_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV3130642.1"
                     /translation="MVIRKKKCRDCGNAITHNTVYCPYCGSVDPFGYYRNTDRIVTIL
                     LALIIVVLLTTVSVSVYILCSW"
     gene            2247..2816
                     /locus_tag="RH057_004386"
     CDS             2247..2816
                     /locus_tag="RH057_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV3130643.1"
                     /translation="MTYKYNPFWQQRIRETVRHALNVHPRLTALRVDLRFPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHPTTLHYVWAREFGECKGKKHYHLMLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDVGCHATLVHFPAWPAVWLERDDDTGFQ
                     QVLERADYLAKEHTKAHCTGERNFGCSRG"
     gene            2983..3384
                     /locus_tag="RH057_004387"
     CDS             2983..3384
                     /locus_tag="RH057_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004020115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130644.1"
                     /translation="MYAKSFIALDGNGRLTGARTAQAAPYANYTCHLCGSALRYHPQY
                     DTERPWFEHTDDGLTAHGQQCPYVRPERREVRLIKRLQQFVPDALPVVRKANWHCRQC
                     HHDYYGEQYCTNCQTGGFSIPRTTQEEICEF"
     gene            3372..>3779
                     /locus_tag="RH057_004388"
     CDS             3372..>3779
                     /locus_tag="RH057_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001556209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HDV3130645.1"
                     /translation="MRILNCYMANDSKGHFVTAKEAAKHNRQDILCCVSCGCPLTLKR
                     GNHGQPPWFEHDQMTVAEKILLRCTWLDPAEKEARRLHLQGMTVPDYTVKVRKWFCVM
                     CDEDYEGEKCCPRCGTGVYSRAWGRQEVPSEDAR"
BASE COUNT          901 a          950 c          999 g          929 t
ORIGIN      
        1 taaatacctt ttctcagaag tggccgtcca ggcggtcacc gaactcgata ataaagcggc
       61 tcattgccat acgccagtcc cgcagtggca tcgtccattt ctgtgaagcc gcctggattg
      121 ccagccacac cacttttttc accgagtcgt ctgtcgggaa cactttacgc tttttgatcg
      181 catggcggat cacgctgttt agcgactcga tggcattcgt cgtatagatc actttgcgga
      241 tgtccgttgg ataagcgaag aacgtggcaa gattcggcca gttagcctgc cagcttcggc
      301 ttatctgagg atagcgacag tcccaggccg cagcgaacgc ttccagtgcc tgctggcctg
      361 cctcttccgt gggagcctga taaatcgctt tcaggtcgcg agtgacggct ttgtagtcct
      421 tccatgacac gaagcgcagg ctgttgcgca ccatatgcac gatgcataac tggatgcggg
      481 ccttcggata tactgtgttg atggcatccg ggaagccttt caggccatcc acacaggcga
      541 tgaggatatc gttcagaccg cggtttttca gttcagtcag cacattgagc cagaacttcg
      601 ccccttcatt ttcggccagc cacataccca gcagttcttt ctgaccttcg atattgatgc
      661 ccagtgccag gaacaccgat ttgttgatga cgcgactgtc ctgccgaact ttcaggacga
      721 tacagtcaag ataaacaatg gggtaaacag catccagtgg tcggttttgc cattctacaa
      781 cctgctccat cacggcatcg gtaacctttg atatcagtgc cggtgaaaca tctgcgtcat
      841 acagttcttt gaacgcagca gctatctcac gggtggtcat ccctttggca tacaacgaga
      901 ggatctggtt atccatcccg gtaatacggg tctgattttt ctttaccagt tgtggttcga
      961 aggtaccgtc acgatcgcgc ggagtacgca gttccagtgg accgtcgcct gtgataacgg
     1021 tctttgtgga aaaaccgttg cgggagttag ctcctggtct ggactgattt ttctcatacc
     1081 caagatggtg tgtcatctct gcattgagag cggcttcaac gctgagcttt ttcagcagcc
     1141 gatcaaactg actgaggtct tcaggggttt tgaggttttt ggccagttcg ttagccagag
     1201 cctgtaactg tttttcgtcc ataaattaac cttcatttga tgctggattg aacatatcaa
     1261 aatcaggcaa ttacacaaat ctatgtacag gctcatttcc cacacgcgtt ataaacttac
     1321 ctctgcggaa ctggaagcct tcaactctgt ggttgatcac tggctggcag aactgacaat
     1381 gaacaagctt tacgatcgtg ttcccgcttc agtctggaaa tatgtcatct gagcgttcgg
     1441 taatcacaac tgcatcgcac caggtgttat ccgctttgtg attcttattg gctcttctca
     1501 ggttattaaa agctgaatat gttcctgttg tcgggaacca ggataatacc gggaacgtta
     1561 agcggtatag cgacaacaaa gaattcgaaa tcaaaaggga atggtgccgg aggcgtgggc
     1621 ggtaatgggc tgattttcag gtattaccgg cttacagcgt gtggttgata ttgaggggct
     1681 gtgagtttca cgtatttgtc agaaacccag acgagttttc ctgtatggga cgaaataagg
     1741 cagcggcaat acctggaaaa atttaatatg gtatgaagga gaacctgtcg gttacgctta
     1801 atataaccgg tacactgtca gctcagggga tacatgaacc gttatctcac cagctacaca
     1861 gtatatacac actgacgctg acagtagtca gcaggaccac aataatcagt gccagcagta
     1921 tggtaacgat acggtctgta ttccggtaat agccgaaggg gtcgacagaa ccacaatacg
     1981 ggcaatacac agtattatga gtaatcgcgt ttccacagtc cctgcacttc tttttgcgta
     2041 tcaccataac gccctcctca cagccttacc cgtaaccggt tttttacatt aaaaaaacaa
     2101 cgctgaaaat tcaaccgtca gtccggagac gaccgttcgg gttatctcag agtgcctgag
     2161 acagtgtcct gccggaggtc acttcaggga ctctgcgcat tttttacgac gtggctattc
     2221 cgtatgaacc atacggagat ttaaccatga cctacaaata caaccccttc tggcagcaac
     2281 gtattcgtga gacggtgcgg cacgcactga atgttcatcc ccgcctgacg gcattgcggg
     2341 ttgacctgcg tttcccggat gtaccggcag caacggacgc agctgtgata tcccgcttca
     2401 tcaatgccct gaaagcccga atcgacgctt accagaaacg taagcatcgg gaaggtaaac
     2461 gcgtgcatcc caccaccctg cattacgtct gggcccggga gtttggggag tgcaaaggta
     2521 aaaaacacta tcacctgatg ctgctggtta accgggatac ctggtgtcgt gccggtgatt
     2581 accgtgctcc gggatcactg gccgggatga ttaaacaggc gtggtgcagt gccctgggag
     2641 tggatgtcgg gtgccatgcc acgctggtgc attttccggc ctggccggcg gtgtggctgg
     2701 aacgcgatga tgacactggc tttcagcagg tgctggaacg tgctgactat ctggcgaagg
     2761 aacataccaa agctcactgc accggtgagc gcaactttgg ctgcagtcgt ggctgagccc
     2821 gacagacgac ttatctgacc ttagtgtgat gacgcgttca cagcgaccac actgattttc
     2881 ccttctgaga ctgaactgaa aaccgctatt cacacctgac cacgttgccc gtaccgggac
     2941 ggctgttgcc tgcgtgtcgt ctggcggctg aggaatatga ctatgtacgc aaaatccttt
     3001 atcgctcttg atggcaacgg acgtctgacg ggcgcccgta ctgcacaggc cgcaccttat
     3061 gctaactaca cctgccactt gtgtggcagt gcactcagat accatccgca atacgacact
     3121 gaacgtccct ggtttgaaca cactgacgac gggctgacag cgcacggtca gcagtgtcct
     3181 tatgtcagac cggaacgcag ggaggtacgc ttgattaagc gtctgcagca attcgtaccg
     3241 gatgccttac ccgtggtgcg taaagccaac tggcactgca gacaatgtca ccacgattat
     3301 tatggggagc agtactgcac aaactgccag accggaggat tcagtattcc ccggacaact
     3361 caggaggaaa tatgcgaatt ctgaactgtt atatggcgaa tgacagcaaa ggccattttg
     3421 ttacggcgaa agaagctgcg aagcacaacc gacaggacat tttgtgctgt gtgtcctgtg
     3481 gatgcccttt aacacttaag cggggcaatc acggacaacc accgtggttt gaacatgacc
     3541 agatgactgt cgctgagaaa atcctgctgc gatgcacctg gcttgacccg gcagagaaag
     3601 aggcccgtcg tttgcatctg cagggtatga cggttccgga ttatacggtg aaggtgagaa
     3661 agtggttttg tgtgatgtgt gacgaagatt atgaaggaga aaagtgctgc ccgcgttgtg
     3721 gtaccggggt atacagcagg gcgtgggggc ggcaggaggt gccgtcggaa gatgccagg
//
LOCUS       DAPHDW010000239         3543 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.244, whole genome shotgun sequence.
ACCESSION   DAPHDW010000239 DAPHDW010000000
VERSION     DAPHDW010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3543)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3543)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3543
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.244"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..261
                     /locus_tag="RH057_004389"
     CDS             <1..261
                     /locus_tag="RH057_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HDV3130646.1"
                     /translation="LWIEHNLDQSLLLDDVANKAGYTKWYFQRLFKKVTGVTLASYIR
                     ARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRVMTPTY"
     gene            complement(255..527)
                     /locus_tag="RH057_004390"
                     /pseudo
     CDS             complement(255..527)
                     /locus_tag="RH057_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            651..2054
                     /locus_tag="RH057_004391"
     CDS             651..2054
                     /locus_tag="RH057_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001157239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HDV3130647.1"
                     /translation="MLEKAEAEEQLRQENDKKLLGQVLEIYDQKYVAELLRKVGKNEW
                     SRETLNRWINGKCSPKTLTSAEEELLRKMLPEAPAHHPNYAFRFIDLFAGIGGIRKGF
                     ETIGGQCVFTSEWNKEAVRTYKANWFNDAQEHTFNLDIREVTLSDKPEVPENDAYAYI
                     NEHVPDHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFECEAQGTLFFDVARIIRAKKPA
                     IFVLENVKNLKSHDKGKTFKVIMDTLDELGYEVADAAEMGKNDPKVIDGKHFLPQHRE
                     RIVLVGFRRDLNIHQGFTLRDISRFYPEQRPSFGELLEPVVDSKYILTPKLWEYLYNY
                     AKKHAAKGNGFGFGLVNPENKESIARTLSARYHKDGSEILIDRGWDMATGETDFANEE
                     NQAHRPRRLTPRECARLMGFEKVDGRPFRIPVSDTQSYRQFGNSVVVPVFEAVAKLLE
                     PYILKAVNADSCKVERI"
     gene            complement(2088..3302)
                     /locus_tag="RH057_004392"
     CDS             complement(2088..3302)
                     /locus_tag="RH057_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_143212653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II site-specific deoxyribonuclease"
                     /protein_id="HDV3130648.1"
                     /translation="MFMSVFHNWLLEIACENYFVYIKRLSANDTGATGGHQVGLYIPS
                     GIVEKLFPSINHTRELNPSVFLTAHVSSHDCPDSEARAIYYNSRHFGKTRNEKRITRW
                     GRGSPLQNPENTGALTLLAFKLDEQGGDCKEVNIWVCASTDEEDVIETAIGEVIPGAL
                     ISGPAGQILGGLSLQQAPVNHKYILPEDWHLRFPSGSEIIQYAASHYVKNSLDPDEQL
                     LDRRRVEYDIFLLVEELHVLDIIRKGFGSVDEFIALANSVSNRRKSRAGKSLELHLEH
                     LFIEHGLRHFSTQAITEGNKKPDFLFPSAGAYHDTEFPVENLRMLAVKTTCKDRWRQI
                     LNEADKIHQVHLFTLQEGVSLAQYREMRESGVRLVVPSSLHKKYPEAVRAELMTLGAF
                     IAELTELYADIP"
BASE COUNT          969 a          830 c          839 g          905 t
ORIGIN      
        1 ttatggattg aacataattt agatcagtct ttactgcttg atgatgtggc gaataaagcg
       61 ggttatacca agtggtattt tcagcggctg ttcaaaaaag taacaggggt cacactggct
      121 agctatattc gtgctcgtcg tttgacgaaa gcggctgttg agttgaggtt gacgaaaaaa
      181 actatccttg agatcgcatt aaaatatcaa tttgattccc aacaatcttt tacacgtcgg
      241 gtaatgactc caacttactg atagtgtttt atgttcaaat aatgcccgat gaccttgtca
      301 tgcaactcca ccgattttga gaacgacagt gacttccgtc ccagccttgc cagatgttgt
      361 ctcagattca ggttatgtcg ctcaatgcgc tgagtgtaac gcttgctgat aacgtgcagc
      421 tttcccttca ggcgggattc atacagcggc cagccatccg tcatccatac cacgacctca
      481 aaggccgaca gcaggcccag aagacgctcc agcgtggcca acgtgcgtta cctaaaacct
      541 gtttaaatat ccagataaaa acattcaatc tgggtcaaat gagtgataca gtttcaccca
      601 taagacccaa tggaggcaat atgtctgaat ttgaattact ggcgcaggat ctgcttgaga
      661 aagcagaagc ggaagaacaa ctgcgacagg aaaatgataa aaagctgctc gggcaggtgc
      721 tggaaatcta tgaccagaag tacgtggctg aactgcttag aaaagttggt aaaaatgagt
      781 ggagtcgcga gactcttaat cgctggatta atggtaagtg ctcacctaag acgctgacgt
      841 cagccgaaga ggaacttcta cgaaaaatgc ttccggaagc gcctgcacat caccctaact
      901 atgccttccg gtttattgac ctgtttgctg ggattggagg tatacggaag ggcttcgaaa
      961 ccatcggtgg ccagtgcgtt tttaccagtg aatggaataa agaggctgtg cgcacatata
     1021 aagctaactg gtttaacgat gctcaggaac acactttcaa tctcgatatt cgggaagtca
     1081 cgctcagtga taaacctgaa gtacctgaaa acgatgccta tgcttacatt aatgagcatg
     1141 tgccggatca tgatgtactt ctagcaggtt tcccctgtca accgttcagc cttgcgggcg
     1201 taagcaagaa aaactcgctc gggcgcgcgc atggtttcga atgtgaggct cagggaacac
     1261 ttttcttcga tgtggcgcgt attatccgcg caaaaaaacc tgccatcttt gttcttgaaa
     1321 acgttaaaaa cctgaagagc catgacaagg gtaaaacctt taaagtcatc atggataccc
     1381 tcgacgaact gggctatgaa gttgcggatg cagctgagat gggcaaaaac gatcctaaag
     1441 ttatcgacgg aaagcacttt ttacctcagc accgagaacg tatcgttttg gtcggtttcc
     1501 gtcgtgatct gaacattcac cagggcttta ccctgcgcga tattagtcgt ttttatccgg
     1561 aacagcgtcc gtcatttggc gaactgctgg aacccgtggt tgacagcaaa tatatactga
     1621 cgccgaaact ctgggagtat ctctataact acgccaaaaa gcacgcagct aagggtaacg
     1681 gattcggttt tggcctcgtt aatcctgaaa ataaagaaag cattgcccgt acgctttctg
     1741 ctcgctatca caaagacggg tctgaaattc tgatagaccg tggctgggat atggccacag
     1801 gtgaaacaga cttcgcgaac gaagaaaatc aggcgcatcg gccccgtagg ctgactccac
     1861 gagagtgcgc gcgccttatg ggttttgaaa aagtagatgg caggcctttt cgcattcctg
     1921 tgtcagacac tcagtcgtac aggcagttcg gtaactccgt agtggtgccc gtgtttgaag
     1981 ccgtagccaa actgcttgaa ccttatatcc tgaaagcggt taatgccgat tcgtgcaagg
     2041 ttgaacgaat ctgatcgctc ctcccggtat ttatgccggg agataatcta tggaatatct
     2101 gcgtaaagct ctgtcagctc agcaataaac gcacctagcg tcattagctc agctctcacc
     2161 gcctccgggt attttttgtg cagagatgat ggcacgacca atctgacacc cgactcccgc
     2221 atctcccgat attgagccag agaaactccc tcctggagtg taaacagatg cacctgatga
     2281 attttatcgg cctcattcag tatctgacgc cagcgatcct tacaggtagt cttgactgcc
     2341 agcatgcgca gattttctac gggaaactca gtatcgtggt aagcccctgc ggaagggaaa
     2401 aggaaatcgg gttttttatt accttctgtg atggcctgcg tcgaaaagtg tcgcaggccg
     2461 tgctcaatga atagatgctc caggtgcagt tccagcgact tcccggctct ggatttacgg
     2521 cgattgctga cagaattggc cagcgcaata aattcatcca cagagccaaa tcctttccgg
     2581 atgatatcca gaacatgcag ttcctcaacc aatagaaata tgtcgtactc cacgcgccgg
     2641 cggtcaagaa gttgctcatc cggatcaagg gaatttttca cataatggct ggctgcatac
     2701 tgaataattt cacttcccga cggaaagcgc aggtgccagt cttcaggtag aatatattta
     2761 tgatttactg gcgcttgctg tagagatagt ccgcctagaa tctgtcctgc ggggccggat
     2821 ataagcgctc cgggtataac ttcaccaata gcggtctcaa tgacgtcctc ttcatcagtg
     2881 ctggcgcata cccaaatatt tacttcctta cagtcccccc cttgctcatc aagcttgaaa
     2941 gctaggagcg tcagagcacc tgtattttca ggattctgaa gtgggctgcc tctaccccag
     3001 cgggtaatcc ttttttcatt ccgggtttta ccaaaatgac ggctgttata ataaattgcc
     3061 cgggcttcgc tgtcagggca atcatgcgat gacacatgtg cggtgagaaa aaccgaaggg
     3121 ttcagttcac gggtatggtt gatagacgga aagagttttt caacgatacc tgaagggata
     3181 taaagtccta cctggtgacc acctgttgcg ccggtatcgt tggcggaaag gcgtttgatg
     3241 tagacgaagt aattctcaca tgcgatctcg agtagccagt tgtggaaaac cgacataaac
     3301 atcccctgtt accctgaaac tctactcacc attttttcat aattatatac aaacagccag
     3361 aaaggctgtt acaggcgatt tgatctgcaa cctattggtt aaattaatgt atcaaaaacg
     3421 atggtttctg tgacacatcc ctataagcag ttgcctatac aataaaacgc tctccggctg
     3481 taacaaaacc cgttacagta tcaatacaac aaaaacgatg taacaaaata gggtggcaaa
     3541 atg
//
LOCUS       DAPHDW010000240         3214 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.245, whole genome shotgun sequence.
ACCESSION   DAPHDW010000240 DAPHDW010000000
VERSION     DAPHDW010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3214
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.245"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            156..1838
                     /locus_tag="RH057_004393"
     CDS             156..1838
                     /locus_tag="RH057_004393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HDV3130649.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFTPTEYSTKGKPNIIVLTMDDLGYGQLPFDKGSFDPKTMENREVVDTYKIGIDKAI
                     EAAQKSTPTLLSLMDEGVRFTNGYVAHGVSGPSRAAIMTGRAPARFGVYSNTDAQDGI
                     PLTETFLPELFQNHGYYTAAVGKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQ
                     NRGFDYFMGFHAAGTAYYNSPSLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPF
                     MLYLAYNAPHLPNDNPAPDQYQKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQ
                     YDNTIILFTSDNGAVIDGPLPLNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKL
                     ISAMDFYPTALDAADISIPKDLKLDGVSLLPWLQDKKQGEPHKNLTWITSYSHWFDEE
                     NIPFWDNYHKFVRHQSDDYPHNPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTD
                     LQQKDNLAAANPQVVKEMQGVVREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1890..3047
                     /locus_tag="RH057_004394"
     CDS             1890..3047
                     /locus_tag="RH057_004394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416014.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HDV3130650.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIAASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNALQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCLKCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(3013..3135)
                     /locus_tag="RH057_004395"
     CDS             complement(3013..3135)
                     /locus_tag="RH057_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130651.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
BASE COUNT          978 a          689 c          641 g          906 t
ORIGIN      
        1 atagaacaga aagtaatgtc aacgctttat ggactgttat ttcccttttt ttagctaaat
       61 ctgctatctc tttatgtgac taacttcact tacatccact tatttctctt cgtaaaatta
      121 cttcggaatt aagtacaata agaagaggaa catttatgaa gtctgcatta aagaaaagtg
      181 tcgtaagtac ctcgatatct ttgatactgg catctggtat ggctgcattt gctgctcatg
      241 cggcagatga tgtaaagctg aaagcaacca aaacaaacgt tgctttctca gactttacgc
      301 cgactgaata cagtaccaaa ggaaagccaa atattatcgt actaaccatg gatgatcttg
      361 gttatggaca acttcctttt gataagggat cttttgaccc aaaaacaatg gaaaatcgtg
      421 aagttgtcga tacctacaaa atagggatag ataaagccat tgaagctgca caaaaatcaa
      481 cgccgacgct cctttcatta atggatgaag gcgtacggtt tactaacggc tatgtggcac
      541 acggtgtttc cggcccctcc cgtgccgcaa taatgaccgg tcgagctccc gcccgctttg
      601 gtgtctattc caatacagat gctcaggatg gtattccgct aacagaaact ttcttgcctg
      661 aattattcca gaatcatggt tattacactg cagcagtagg taaatggcac ttgtcaaaaa
      721 tcagtaatgt gccggtaccg gaagataaac aaacgcgtga ctatcatgac aacttcacca
      781 cattttctgc ggaagaatgg caacctcaaa accgtggctt tgattacttt atgggattcc
      841 acgctgcagg aacggcatat tacaactccc cttcactgtt caaaaatcgt gaacgtgtcc
      901 ccgcaaaagg ttatatcagc gatcagttaa ccgatgaggc aattggcgtt gttgatcgtg
      961 ccaaaacact tgaccagcct tttatgcttt acctggctta taatgctccg cacctgccaa
     1021 atgataatcc tgcaccggat caatatcaga agcaatttaa taccggtagt caaacagcag
     1081 ataactacta cgcttccgtt tattctgttg atcagggtgt aaaacgcatt ctcgaacaac
     1141 tgaagaaaaa cggacagtat gacaatacaa ttattctctt tacctccgat aatggtgcgg
     1201 ttatcgatgg tcctctgccg ctgaacgggg cgcaaaaagg ctataagagt cagacctatc
     1261 ctggcggtac tcacacccca atgtttatgt ggtggaaagg aaaacttcaa cccggtaatt
     1321 atgacaagct gatttccgca atggatttct acccgacagc tcttgatgca gccgatatca
     1381 gcattccaaa agaccttaag ctggatggcg tttccttgct gccctggttg caagataaga
     1441 aacaaggcga gccacataaa aatctgacct ggataacctc ttattctcac tggtttgacg
     1501 aggaaaatat tccattctgg gataattacc acaaatttgt tcgccatcag tcagacgatt
     1561 acccgcataa ccccaacact gaggacttaa gccaattctc ttatacggtg agaaataacg
     1621 attattcgct tgtctataca gtagaaaaca atcagttagg tctctacaaa ctgacggatc
     1681 tacagcaaaa agataacctt gccgccgcca atccgcaggt cgttaaagag atgcaaggcg
     1741 tggtaagaga gtttatcgac agcagccagc caccgcttag cgaagtaaat caggagaagt
     1801 ttaacaatat caagaaagca ctaagcgaag cgaaataact aaaccttcat gcggcggaat
     1861 tttccgccgc cttattgagc gagatagcga tgcacgttac agccaagcca tccagttttc
     1921 aatgtaatct caaatgtgat tactgttttt accttgaaaa agagtcgcag tttactcatg
     1981 aaaaatggat ggatgacagc actctgaaag agttcatcaa acaatatatc gcagcgtctg
     2041 gcaatcaggt ctattttacc tggcaaggcg gtgaacccac tctggctggc ctggattttt
     2101 tccgtaaagt tattcactat caacaacgct atgcaggcca aaaacgtatt tttaatgcat
     2161 tacaaacgaa tggcatttta ttgaataatg aatggtgtgc ttttctcaaa gaacatgagt
     2221 ttctggttgg tatctcgatc gatggccccc aggagctaca tgaccgttac agacgcagta
     2281 attcaggtaa cggtactttt gcaaaagtga tagcagccat cgagcgtctg aaatcatatc
     2341 aagttgagtt taatacgtta accgtcatta ataacgttaa tgtccattac cctcttgagg
     2401 tttatcattt tttaaaatct atcggcagta aacatatgca atttatcgaa ttgctcgaaa
     2461 ccgggacgcc gaatattgat ttcagtggtc atagtgagaa cacattccgt atcattgatt
     2521 tttctgtgcc tcccacggct tatggcaagt ttatgtcaac catttttatg caatgggtta
     2581 aaaacgatgt gggtgaaatt ttcatccgtc agtttgaaag ctttgtcagc cgttttttag
     2641 ggaatgggca taccagttgt attttccagg agtcctgcaa ggataatctg gttgttgaaa
     2701 gtaatggaga tatttatgaa tgcgaccatt ttgtctatcc acagtacaaa attggaaaca
     2761 ttaataaatc tgaactcaaa acgatgaaca gtgtacaact gacagcgcaa aaaaaacgga
     2821 tttcagcgaa atgtctgaaa tgtgcatata aacctatctg caatggcggt tgtcctaagc
     2881 atcgtattac taaagtaaac aatgagactg tttcttattt ttgcgaaggt tataaaatcc
     2941 ttttttcaac catggtacct tatatgaacg ccatggtgga gttagctaag aacagagtac
     3001 cgctttacca cattatggat gttgcaaaac aaatggagaa taattaataa cgctctcttt
     3061 taactccatt cttagcctcc ttcttatgac cttaacaagg cacccgacat aaattcctgt
     3121 cgggtgtttc gtcatcatca tcttcccttg cgaaaatcct ggcttacgtg gtatgcatca
     3181 gggcattagc aatatccttg attatcaata gcat
//
LOCUS       DAPHDW010000241         3180 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.246, whole genome shotgun sequence.
ACCESSION   DAPHDW010000241 DAPHDW010000000
VERSION     DAPHDW010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3180)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3180)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3180
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.246"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            180..308
                     /locus_tag="RH057_004396"
                     /pseudo
     CDS             180..308
                     /locus_tag="RH057_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308261.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tube protein Hcp"
     gene            306..2075
                     /gene="tssI"
                     /locus_tag="RH057_004397"
                     /pseudo
     CDS             306..2075
                     /gene="tssI"
                     /locus_tag="RH057_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
     gene            2151..>3180
                     /locus_tag="RH057_004398"
     CDS             2151..>3180
                     /locus_tag="RH057_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS element protein"
                     /protein_id="HDV3130652.1"
                     /translation="MGGGKPAARQGDMTRKGLDIVQGSAGVLIGAPTGVACSVCPTKK
                     DSPNYGSPVNPLLGAKVLPVETDLALPGPLPFILFRAYSSYRTRTPAPVGVFGPGWKA
                     PFDIRLQIRDEGLILNDSGGRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLS
                     ALWQVLPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPPPPAYRVLTGVVD
                     GFGRTLAFHRAAKGDVAGAVTGVTDGAGRRFHLALTTQAQRAEAFRKQRASSLSSPAS
                     PRSVSSSQVFPDTLPAGTEYGADNGIRLEAVWLTHDPAYPDEQPTAPLARYTYTAGGE
                     LRAVYDRSG"
BASE COUNT          746 a          830 c          991 g          613 t
ORIGIN      
        1 ataagagatt taatgatttt aaaaatagtt tttacgacaa ccgtaaattc tagctcggtt
       61 tgtatgtaaa aaatactgaa aaatgatgcg gttccgtgaa gtgagctgca tctgcctgtc
      121 tgggattaac ggcaggtcat tgtaagtagt aattaaccac gttaacaagg agtttacaaa
      181 tgccaacacc gtgttatatc tccatcactg gtcagaccca gggaaatatt actgcaggtg
      241 catttaccgc tgattctgtc ggcaacatct atgtgcaggg gcacgaagac gagatgctgg
      301 tgcaggaaga aaacggtgtg accgagtgga gtccgctgtt cagcgagccg catccttccc
      361 gtgagttttg tgtccagtac ggcgaaaccg attacgattt cctgtgccgg atggcggcgg
      421 aggaaggcat cttcttttat gaggagcacg cgcaaaaaag taccgaccag agcctggtcc
      481 tgtgcgacac cgtgcgttat ctgccggagt cctttgagat cccctggaat ccgaacaccc
      541 gtaccgaggt gagcaccctc tgcatcagcc agttccgcta cagcgcacaa atccgtcctt
      601 cttccgtggt gacaaaagac tacaccttta aacgtcccgg ctgggcaggg cgttttgacc
      661 aggagggcca gcaccaggac taccagcgca cgcagtatga agtgtatgac taccccggac
      721 gtttcaaggg cgcccacggg cagaactttg cccgctggca gatggacggc tggcgaaaca
      781 acgcagaagt ggcgcgcgga acaagccgtt cgccggagat atggccggga cggcgaattg
      841 tgctgacggg gcatccgcag gcgaacctga accgggaatg gcaggtggtg gcgagcgatc
      901 ttcacggcga acagccgcag gcgataccgg gacgcagggg ttcaggcacc acgctggata
      961 accattttgc ggtaataccg gcagacagaa catggcgacc acagccgttg ctgaaaccgc
     1021 tggtggacgg cccgcagagc gccgtcgtga cgggaccggc aggcgaggaa atcttctgtg
     1081 atgaacatgg ccgcgtgcgg gtgaaattta actgggaccg ttataacccg tcaaaccagg
     1141 acagttcatg ctggatccgt gtggcacagg cgtgggcagg caccggattc ggtaacctgg
     1201 cgataccgcg tgtgggtcag gaggtgattg tggacttcct caacggcgat ccggaccagc
     1261 cgatcattat ggggcgcacc taccaccagg aaaaccgcac acccggcagc ctgccgggaa
     1321 caaagacgca gatgaccatt cgttcgaaaa cctataaggg cagcgggttt aatgaactga
     1381 agtttgacga tgcgacaggg aaagaacagg tctacatcca cgcgcagaag aacatgaaca
     1441 ccgaggtgct gaataaccgc accactgatg tgataaacaa ccatgccgaa aaaataggta
     1501 acaaccaggc gatcaccgtt accaataacc agatccagaa cattggcgtt aatcagatac
     1561 agacggttgg tgtcaaccag gtggaaacgg tgggcagtaa ccagattatc aaagtgggat
     1621 caaaccaggt tgaaaaggtg gggatcattc gtgcgctgac ggtgggtgta gcttaccaga
     1681 cgacggtagg cggcattatg aatacctcgg tggcgttgtt gcagtcctca caggtagggc
     1741 tgcataaatc actgatggtg ggaatgggct acagcgtcaa tgtggggaat aacgtcacct
     1801 tctcggtggg caagacgatg aaggaaaaca ccggacaaac agcagtttat tctgccggtg
     1861 agcatcttga actctgctgt ggtaaggcaa ggctggtgct gacgaaggac ggaagcatat
     1921 ttctcaacgg tacgcacatt catctggaag gggagtcgga tgtgaacggt gatgcgccag
     1981 tgattaactg gaactgtggt gccacacaac ctgtaccgga tgcgcctgtg ccgaaagatt
     2041 taccccccgg aatgccggat atgcggcaat tttgagtcag gtgcaaaaaa ttaaaacgga
     2101 actggtgcag tgaccagatg aaccatatta aaagcagaag gaattgattt atgggcggcg
     2161 gaaaaccggc ggcgcggcag ggtgacatga cccgcaaggg actggatatt gtgcagggtt
     2221 cagcaggggt gctgataggt gcgccaaccg gtgtggcctg ctcggtgtgt cccacaaaaa
     2281 aggacagtcc gaactatggc agcccggtga acccgctact cggtgcgaag gttctgcctg
     2341 ttgaaaccga cctggcactg cccggcccgc tgccgtttat tcttttccgc gcctacagca
     2401 gctaccggac caggacgccc gcgccagtgg gcgtgtttgg tcccggctgg aaagcgccct
     2461 tcgatatccg cttacagata cgcgatgaag gcctgatact caacgacagc ggtggtagga
     2521 gcattcactt tgagccgttg tttcccggcg agataagcta cagccgcagc gagtcactct
     2581 ggctggcgcg gggcggagta gcggcgcagc acagttcgca gccgctaagc gcgctctggc
     2641 aggtgctgcc ggaagatgtt cgcctgagtc cgcatgtgta cctggcgaca aacagccttc
     2701 aggggccgtg gtggatactc agctggccgg agcgggtgcc gggggcggac gaggtgctgc
     2761 cgcctccgcc gcccgcatac cgggtgctga cgggggtggt ggacggcttc gggcggacgc
     2821 tggcctttca ccgggcagct aagggtgacg tggcgggcgc ggtgacgggg gtgacggacg
     2881 gcgcgggccg ccgttttcac ctggcgctga ccacgcaggc gcagcgggcg gaagccttcc
     2941 gtaaacagcg cgcctcttcc ttatcttccc cggccagtcc ccgttctgtt tcctcttctc
     3001 aggttttccc cgacacgctg cccgccggta cagagtacgg tgccgataac ggtatccggc
     3061 tggaggcggt atggctgaca cacgacccgg catacccgga tgaacagccg actgcgccgc
     3121 tggcgcgcta cacgtacacg gccggtggtg aactgcgggc ggtgtatgac cgcagcggga
//
LOCUS       DAPHDW010000242         3106 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.247, whole genome shotgun sequence.
ACCESSION   DAPHDW010000242 DAPHDW010000000
VERSION     DAPHDW010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.247"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1822)
                     /locus_tag="RH057_004399"
     CDS             complement(<1..1822)
                     /locus_tag="RH057_004399"
                     /inference="COORDINATES: protein
                     motif:HMM:NF015188.1,HMM:NF027494.1,HMM:TIGR04415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV3130653.1"
                     /translation="MNRFSQIVWNESTGEVGSDLSTGQEGQQQVKIRQTVLAPLIASF
                     LLTPSSVLAENYSNETLPNSTTAGSVLNEGDIATNTTINSGGHQYVYSGGSATDTLIS
                     GGDQHVSSGGSAINTIINGLHYKDGSQYVSSGGNAINTIINDEGEQYVYNGGNAISTT
                     INSRGVQVVSNGGIATNTTINGGGQIVYSGGKVSVSRLNSGHQYVYSGGSATDIIIDR
                     FGNQIVYSGGRVSNTTINGGEQLVIGGHAISTTINSGGKQTVHTSGSVTSTTINNGGV
                     LWVGSYSTGTDIVQNDGGAIITDTDETVSGFNTNGEFSIADGRASNMLLENGGSLTVL
                     PGHSVKDTMVGSGGQLGVVKDILLLGNNTIADSGKIVSDEITLSITNNGHLHFLNNSA
                     ATYTFDLTGTGDLKVDGGHLVLSGTLSQDGGVSIVNSGTMVMDNLQATADLTMQADTS
                     LSLEQNSTLAGRIVGDGAGAGNVTVRSSLWELRGDSTVGALTMDNGTVHFHSPQSENW
                     QPAILTAGSLSGNGTFRMNTDIAAHTGDLLNVNGNASGNFVLNIRNTGQEPASAGSPL
                     RVVHTGSGDARFTLDGGKVDAGTWEYYLNKENTDWYLKADV"
     gene            complement(1815..3026)
                     /locus_tag="RH057_004400"
     CDS             complement(1815..3026)
                     /locus_tag="RH057_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008502399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter strand-loop-strand
                     O-heptosyltransferase"
                     /protein_id="HDV3130654.1"
                     /translation="MKTSFIYPPEIPTQSGPKHICYDFNDGARVLLPDGKWHVRLIDD
                     DSGNILFQCDSGKGWVTSTKKYFVRFRIQVYQQGSTHPILDETLNLTDKPVLISFPTG
                     TLGDLLGWFPYAERFQKQHQCRLECAMAQNIIDLLASQYPQIHFSTPDKLQTVLPYAT
                     YRVGLYFGGDTDNQPVDFRQVGFHRNAGYILGVDPREEPVRLDLSSPRTIQEPYVCIA
                     TQSTCQAKYWNNGTGWSEVVAHLKSLGYRVLCIDQYAHHGEGLIWNHIPWGTEDFTGS
                     HPLQERVNLLRHASFFIGLSSGLSWLAWATRIPVVLISGFSLPGSEFYTPWRVFNSHG
                     CNGCWDDTSLNFDHKDFLWCPRHKDTPRQYECTRLITGRQVNGVIDRLHLALAEQNGS
                     VTRDKKGVTDE"
BASE COUNT          819 a          798 c          652 g          837 t
ORIGIN      
        1 tgacatcagc tttcagatac cagtcggtat tttctttatt cagatagtat tcccatgtac
       61 ctgcatcaac ctttccaccg tccagtgtaa atctggcatc accactgcct gtatgaacaa
      121 ctcgcagcgg cgagcctgca gaagctggtt cctgtccggt atttctgata ttcagcacaa
      181 agttaccgct ggcatttcca tttacattca acaaatctcc ggtgtgagca gcaatatccg
      241 tattcatcct gaaagttccg ttcccagaca gacttccagc cgtcagaatt gcaggctgcc
      301 agttttctga ctgcggagaa tgaaaatgaa cagtgccatt atccattgta agtgcgccaa
      361 cagtggagtc tcccctcagt tcccataagg aactccgcac agtcacattt cctgcaccag
      421 cgccatctcc cacaatacgt cctgccaatg tgctgttctg ctccagactc aggcttgtgt
      481 cggcctgcat cgtaagatct gctgttgcct gcagattatc cataaccata gtgccactgt
      541 tcacgatgga gaccccacca tcctgtgaca gagtaccact gagcaccagg tgacccccat
      601 ccactttcag gtccccagtt cctgtcagat caaaagtata agttgctgca ctgttgttaa
      661 ggaaatgcaa atgtccgttg tttgtaattg aaagagttat ctcatcactt acaatcttac
      721 cactgtctgc tatcgtgtta ttgccaagca gcaagatgtc cttaacgacc ccgagttgac
      781 caccactgcc taccatggta tctttaacag aatgtcctgg aaggacagtc agactaccac
      841 cgttctccaa cagcatattg ctggcacggc catcagcgat actgaattcg ccattagtat
      901 tgaagccgct tacggtttca tccgtatctg tgataatagc ccctccatcg ttctgaacta
      961 tgtctgttcc agtggaataa ctcccgaccc acaaaacccc accattgtta atggtggtag
     1021 atgtcacact gccgctggta tgaacagttt gtttaccacc actattaatg gtggtagaga
     1081 tggcatggcc cccaatgaca agttgctccc ctccgttaat tgtcgtgttt gagacgcgac
     1141 caccagagta aacgatctgg tttccaaaac ggtcaatgat aatgtctgtg gcactgcctc
     1201 cactatagac atactggtgc ccactgttca gtctggaaac tgagactttg ccaccgctgt
     1261 aaacaatctg cccaccaccg ttaatggtag tgtttgtggc aatgcctccg ttggaaacaa
     1321 cttggactcc gcgactatta atagtggtag atattgcatt gccaccattg tagacatact
     1381 gctccccttc atcgttaatt atagtgttta tagcattacc accgctggag acatactgac
     1441 taccatcctt gtaatggaga ccattgatga tagtattgat ggcgctgccg ccgctggaga
     1501 catgctggtc tccgccacta attaatgtgt ctgtagcact gcctccacta tagacatact
     1561 ggtgcccacc gctgttaatg gttgtattcg ttgctatatc accttcattc aatactgagc
     1621 cagcagtagt gctgttgggc agagtttcat tgctgtagtt ttctgccaga acagatgatg
     1681 gcgtcaacag gaaactggca atcaatggcg ccagtacagt ttgtctgatt tttacttgtt
     1741 gttgaccttc ctgaccagtg ctgaggtcac tacccacttc ccctgtggat tcattccata
     1801 ctatctggga aaacctattc atcagttact ccttttttat cacgtgtcac cgaaccattc
     1861 tgttctgcaa gtgcaagatg aagtctgtcg atcacaccat taacctgcct gccagttatc
     1921 agtcgggtgc attcatactg tcgtggggta tctttatgcc tcggacacca gagaaaatcc
     1981 ttatggtcaa aattcagtga tgtatcatcc cagcaaccgt tacaaccatg ggaattaaac
     2041 acacgccacg gggtatagaa ctcgctcccc ggaagactga aaccacttat cagtaccacc
     2101 ggaatacggg ttgcccacgc cagccatgac agaccactgg aaagaccaat aaaaaaactg
     2161 gcatgtcgaa gcagattaac ccgctcctgc aaagggtgac tgccagtaaa atcctcagta
     2221 ccccacggaa tatggttcca tatcaaacct tctccgtgat gagcatactg gtcaatacac
     2281 aatacccggt aacccagaga tttcagatgt gccaccacct cactccagcc tgtaccgttg
     2341 ttccagtatt tcgcctgaca ggtggactgg gtagcaatac acacataagg ctcctgaata
     2401 gttcttggtg atgacaagtc aagacgtacc ggttcttcac gaggatcaac cccaagaatg
     2461 tagccagcat tacggtgaaa acctacctga cggaaatcca ctggctggtt gtctgtgtct
     2521 ccgccgaaat acagcccaac tcggtaagta gcataaggta aaactgtctg tagtttatct
     2581 ggcgtggaaa aatgtatctg aggatattga gatgccaaca ggtcgataat attctgtgcc
     2641 atcgcgcatt ctaaccggca ttgatgctgt ttctgaaaac gttcggcgta agggaaccaa
     2701 ccgagtaaat ctccaagagt accagtggga aatgaaatca gcacgggctt atctgtcaaa
     2761 ttcagggtct cgtccagtat tggatgtgtt gagccttgtt gatatacctg aatacggaaa
     2821 cgcacaaaat atttctttgt gctggtgacc cacccttttc cagaatcaca ctgaaaaaga
     2881 atgttaccag aatcatcatc tatcagacgt acatgccatt taccatcagg cagaagcacg
     2941 cgggctccat cattaaagtc ataacaaata tgcttaggtc cgctctgggt tggaatttct
     3001 ggtgggtaaa taaaagatgt tttcattttg tatctggata actcgcagta attaatggat
     3061 gcgggctcat taaacataat gaattcaatt gctgaactgg ctgcca
//
LOCUS       DAPHDW010000243         2953 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.248, whole genome shotgun sequence.
ACCESSION   DAPHDW010000243 DAPHDW010000000
VERSION     DAPHDW010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.248"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            199..477
                     /locus_tag="RH057_004401"
     CDS             199..477
                     /locus_tag="RH057_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130655.1"
                     /translation="MAHDTKLHNSDDSAVFASRCGRRFHAFKSDWFQHPPCTEEQAEW
                     IIQCYRRRGYEVKKALSLDYRHWIISVRLPYSERPPRPSHTFQQRIWR"
     gene            479..1528
                     /locus_tag="RH057_004402"
     CDS             479..1528
                     /locus_tag="RH057_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HDV3130656.1"
                     /translation="MRVLLRPVLVPELGLVIVKPGRESMPVFHNTRLLVEPEPKSMRN
                     LPSGVVPAARQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQVAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLALLPHAHQEQHPLQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYTRWVKTQPC
                     ACCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDL
                     IFRFLDHAFATGVLG"
     gene            1541..1915
                     /locus_tag="RH057_004403"
     CDS             1541..1915
                     /locus_tag="RH057_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HDV3130657.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRMGVKIYPIMHEEQVKK"
     gene            1912..2733
                     /locus_tag="RH057_004404"
     CDS             1912..2733
                     /locus_tag="RH057_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HDV3130658.1"
                     /translation="MKLEDLPKYYSPKSPCLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPLVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESVVFTNKVQYPDGKPPVWAKCTKGVYPSYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            2877..2952
                     /locus_tag="RH057_004405"
     tRNA            2877..2952
                     /locus_tag="RH057_004405"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2910..2912,aa:Ile,seq:cat)
BASE COUNT          746 a          685 c          807 g          715 t
ORIGIN      
        1 tggtgatctg gtaaaattgc ataacattaa aatttaattt atctaatcgc ttttaataat
       61 aagcgttgtg tttatcccaa caatctgttg tttgactttt attccattaa tgtaggggct
      121 ttacactgga accagtttat ttatacttta tacgccagcc tgaacaactg gcacctgctg
      181 cgccagcaga gacaaccgat ggcgcacgat accaaattac acaattctga tgattctgcc
      241 gtctttgcca gcaggtgcgg acggcgtttt catgcattca aatcggactg gttccagcat
      301 ccaccatgca ctgaagagca ggctgaatgg ataattcagt gttaccgcag gcgcggatac
      361 gaggttaaaa aagcccttag cctcgactac cgtcactgga taatctcagt cagattgcct
      421 tactccgaac gcccaccgcg tccgtcccac acattccagc aacgcatctg gaggtaacgt
      481 gcgggtatta cttcgacctg ttctggtacc ggaactcggg ctggtgatcg ttaagccggg
      541 ccgtgaatcc atgccggtat tccacaatac ccggttactg gtggagccgg aaccgaaaag
      601 catgcgtaat ctgccgtccg gggtcgttcc tgccgctcgc cagccgctgg tggaagacaa
      661 aacattgctg ccgtttttca gtaacgcacg ggtgattcgt gctgctggtg gtgctggtgc
      721 attgtctgac tggctgttgc gccatattaa atcctgccag tggccacacg gcgattatca
      781 tcacagcgaa accgtcattc accgttatgg taccggcgca atggtgttgt gctggcactg
      841 cgacaaccag ctgcgtgacc agacatccga atcactcgag caagttgctc atcaaaacct
      901 gtcagcatgg atgattgacg tcatcggtca cgcaataagc ggtacgcagg agcgtgaatt
      961 atctctggct gaattatcct ggtgggcggt ccgcaatcag gtggcggacg cgctaccgga
     1021 agcggtatta cgtcgttcgc tggggttgcg tgcggaaaaa atccgctcaa tgtaccgtga
     1081 aagcgacatc gtaccgggag agcagaccgc caccagcata ctgaaacagc gcacaaaaaa
     1141 tcttgcactg ttgcctcacg cccaccagga acaacaccca ctacaggaaa agacggtggt
     1201 aagcattgcc gttgatcctg agtctccgga atcttttatg aaacgaccta aacgtcgccg
     1261 ctgggttaac gagaaataca cacgctgggt gaagacacag ccgtgtgcgt gttgtggtaa
     1321 gccagccgac gatccccatc acctgattgg tcatggtcag ggcggaatgg ggacaaaatc
     1381 tcacgatatt ttcacgctac cgctgtgtcg ggagcatcac aacgagcttc atgcggatcc
     1441 gctggcgttc gaagaaaagc atggttctca ggttgattta atctttcgtt ttcttgatca
     1501 cgcctttgca actggcgtgc ttgggtaaaa gaggtgactg atgctcatag atttggtttt
     1561 accttacccg ccgacggtga acacttactg gcgacgccgt ggcagcacat attttatctc
     1621 ggaggaggga aagcgttatc gccgggctgt ggcgcttatt gttcgccagc agcggctgaa
     1681 attaagcctg tccggaaggc tggcgataaa ggtgattgca gagccaccgg ataagcgtcg
     1741 tcgcgacctg gacaatatcc ttaaagcacc gctggatgcg ctgacgcatg cgggagtgtt
     1801 aatggacgat gagcagtttg atgaaatcaa tatcgttcgt ggtcagccag tatctggtgg
     1861 acgtatgggg gtgaagattt accccataat gcatgaagag caggtcaaaa aatgaaactg
     1921 gaagatttac cgaaatacta ctccccaaaa tccccttgcc tgaccgatgc atcggcctca
     1981 acgtcaaaag atgcgctgag tatcactgat gtgatggccg cgcagggcat gacacagaat
     2041 cgggctgaga tggggttttc tgcgttcctg gggaaaatgg gcatcagtat gaatgacagg
     2101 gcgcgggcaa cagaattact ggcagattat gcactcagtc ggtgcgatcg tgtggcggcg
     2161 ttgagaaaac ttccggcaga aataaaaccg ttagtgatgc gcattatggc ttcgtacgct
     2221 tttgaggatt atgcccgcag cgcagcgagt aaaaagcagt gcccttgttg ctatggggaa
     2281 aaatttattg aaagcgtagt ttttacaaac aaggtccagt atccggatgg taagccgccg
     2341 gtatgggcaa agtgtacgaa aggtgtgtat ccgtcttact gggaagaatg gaaaaaagtc
     2401 agggaggtgg taaaagttgc ctgtccggag tgtggcggaa agggtgaggt ttccaccgcc
     2461 tgtaaggatt gccgtgggcg tggtgtcgcc attcaccgtg aagagtcggt aaaacgtggt
     2521 atgcctgtta tcagagactg ccagcgttgt ggtggtcgtg gctatgaaag actaccatca
     2581 acggaggcat ttaatgctat atgcgaggtg acaaaccaga taacacgcgc gtcatgggaa
     2641 aaaacagtta agaaattcta tgatgcgctg gtgacccggt ttgatattga agaagcatgg
     2701 gctgagcggc agttaaaaaa ggtaactagg taacaaggtt gatttttccg gaatctgtgg
     2761 taaattcgtc ataaagatgg gctttttata cctgacgtta gaagagtttc tacaacccgc
     2821 caccgagcgg gttttttatt gcggaattaa ttacggaccg ttattattct gctcccggtc
     2881 ctttagctca gtggtgagag cgagcgactc ataatcgcca ggtcgctggt tcaaatccag
     2941 caagggccac cat
//
LOCUS       DAPHDW010000244         2931 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.249, whole genome shotgun sequence.
ACCESSION   DAPHDW010000244 DAPHDW010000000
VERSION     DAPHDW010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.249"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..448)
                     /locus_tag="RH057_004406"
                     /pseudo
     CDS             complement(1..448)
                     /locus_tag="RH057_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(442..753)
                     /locus_tag="RH057_004407"
                     /pseudo
     CDS             complement(442..753)
                     /locus_tag="RH057_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308633.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            complement(781..2682)
                     /locus_tag="RH057_004408"
     CDS             complement(781..2682)
                     /locus_tag="RH057_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HDV3130659.1"
                     /translation="MSTGLRFTLEVDGLPPDAFAVVSFHLNQSLSSLFSLDLSLVSQQ
                     FLSLEFAQVLDKMAYLTIWQGDEVQRRVKGVVTWFELGENDKNQMLYSMKVHPPLWRA
                     GLRQNFRIFQNEDIKSILGTMLQENGVTEWSPLFSEPHPSREFCVQYGETDYDFLCRM
                     AAEEGIFFYEEHAYKSTDQSLVLCDTVRHLPESFEIPWNPNTRTEVSTLCISQFRYSA
                     QIRPSSVVTKDYTFKRPGWPGRFDQEGQYQDYQRTQYEVYDYPGRFKGAHGQNFARWQ
                     MDGWRNNAEVARGTSRSPEIWPGRRIVLTGHPQANLNREWQVVASELHGEQPQAVPGR
                     SGSGTTLNNHFAVIPADRTWRPQPLLKPLVDGPQSAVVTGPAGEEIFCDEHGRVRVKF
                     NWDRYNPADQDSSCWIRVAQAWAGTGFGHLAIPRVGQEVIVDFLNGDPDQPIIMGRTY
                     HQENRTPGSLPGTKTQMTIRSKTYMGSGFNELKFDDATGREQVYIHAQKNMDTEVLND
                     RTTTVKHDHRETVKNDQTVTIQEGNRLLTVEKGHKITGVLKGSLSEDVFQDRGTIAGS
                     VHVDAVNNGGEGDGIQAYTAIKEILLAVEESKIALTPDGIQLQVGESTVIRLSKDGIT
                     IVGGSVFIN"
BASE COUNT          633 a          807 c          728 g          763 t
ORIGIN      
        1 gactcttttc actgattgcg taactgtcat gctctggatt atttaattct ttcagcggaa
       61 atttttcctc aaaggcttta tttagccgcc cctggtttcc ttttaccgcg aataaataat
      121 cacctccctg tttttgtatc ttctctgcaa tatctttctg gcaacccatc gcatcagttg
      181 tgatgatttt tcctttaata tccagcatgt taagaagttc tgggatagct gtaatctcat
      241 tagatttctc atccgtcttg atctgtccga tgaccagact gtgcattgtt gagaacgcac
      301 taatgacatg aatcgctccc ctgcggcgac tcttgtcata agagtgccgg agcgtttttc
      361 catcaattgc aatgacgtct ttatcatttg atgaatggca atcacgcatc cagttaataa
      421 agcactcgtg aaattttgca ggactgatat ctggctaatc cgcccctgat tcccctcatc
      481 ttccggaccg ttccaggaat aatccagcag ccaggccggg gcctctgcca gagtgatttc
      541 ccggcaggca tgttcggtgt acccatcaag tttgccctgg tacagcgcca gcgtttcctg
      601 gtacagctct ggtgtcaggg ttccctgtgg aacggcttcg cgggtaatga ccaggttgat
      661 gccactgtta tctgtggaca ccaggacctg cattgactga tcctgccatt gagacggcag
      721 ttgaatgtgg ccttcattaa aagtgaactg cataaaatac tccctgataa tctgagtgaa
      781 tcagttgatg aaaacagaac cgcccacgat ggtgatgcca tccttcgaca gcctgattac
      841 cgtcgattcc ccgacctgta gctgaatgcc atccggcgtc agcgcaattt tgctttcctc
      901 cacggccagc aaaatttcct taatcgccgt ataagcctgt ataccgtcgc cttcgccacc
      961 attgtttaca gcgtcaacat gcaccgaacc ggcaatcgtg cctctgtcct gaaagacatc
     1021 ctcagataaa gaccctttca gtactccggt gatcttgtgg cctttttcta ccgtaagaag
     1081 gcggttacct tcctggatcg tgaccgtctg gtcatttttt acggtttcgc ggtgatcgtg
     1141 ttttacggtg gtggtacggt cgttgagcac ttcggtatcc atgttcttct gcgcgtggat
     1201 gtagacctgt tctctccctg tcgcatcatc aaacttcagc tcattaaatc cgctgcccat
     1261 gtacgttttg gagcggatgg tcatctgcgt ctttgttccc ggcaggctgc cgggggtgcg
     1321 gttttcctgg tggtaggtgc gccccataat gatcggctgg tccggatcgc cgttgaggaa
     1381 gtctacaatc acctcctgac ccacacgcgg tatcgccagg tggccaaaac cggtgcctgc
     1441 ccacgcctgt gccacacgga tccagcacga actgtcttgg tctgccgggt tataacggtc
     1501 ccagttgaac ttcacccgca cgcgaccatg ttcgtcgcag aagatttcct cgcctgccgg
     1561 tcctgtcacg acagcgctct gcgggccgtc caccagcggt ttcagcaacg gctgtggtcg
     1621 ccatgttctg tctgccggga tcaccgcaaa gtgattattc agcgtggtgc ctgaaccact
     1681 gcgtcccggc accgcctgtg gctgttcgcc gtgcagttca cttgccacca cctgccattc
     1741 ccggttcagg ttcgcctgcg gatgccccgt cagcacaatt cgccgtcccg gccatatctc
     1801 cggcgaacgg cttgttccgc gcgccacttc tgcgttgttt cgccagccgt ccatctgcca
     1861 gcgggcaaag ttctgcccgt gggcaccctt gaaacgtccg gggtagtcat acacttcata
     1921 ctgcgtgcgc tggtaatcct ggtactggcc ttcctgatca aaacgccctg gccagccggg
     1981 tcgtttaaag gtgtagtcct tggtcaccac ggaagaaggg cggatttgtg cgctgtagcg
     2041 gaactggctg atgcagaggg tgctcacctc ggtacgggtg ttcgggttcc atgggatttc
     2101 aaaagattcg ggcagatggc ggactgtgtc gcacagcacc aggctctggt cggtactttt
     2161 gtaagcatgc tcctcataaa agaagatgcc ttcctccgcc gccatccggc acaggaaatc
     2221 gtaatcagtc tcaccgtact ggacacaaaa ctcacgggaa ggatgcggct cgctgaacag
     2281 cggactccat tcggtcaccc cgttttcctg caacatcgtg ccgaggatgc ttttgatgtc
     2341 ctcgttctgg aagatacgga agttctggcg cagaccggca cgccacagcg gcgggtgcac
     2401 cttcatgctg tacagcatct ggtttttgtc gttctccccg agttcaaacc aggtcaccac
     2461 gcctttcacc cggcgctgta cttcatcgcc ctgccatatc gtcaggtagg ccattttgtc
     2521 cagcacctgc gcaaattcaa gggagagaaa ctgctggctg accagggaga gatcgaggga
     2581 aaagagcgaa gagagtgact ggttcagatg aaaggaaacc accgcaaaag catccggcgg
     2641 caggccgtcc acttccagtg tgaaacgtaa tccggttgac atagcaacct ccagtaaaat
     2701 gaataatgat ccgtaccgta caactgcgga taatgtgata agcgggacga cttcagacgt
     2761 tattagggaa ggaacagaaa atgctatggt tatgtgagaa ttttgtttaa atattacttt
     2821 acagaaacgg gtgtctgttc agtcaaaaaa ataatcacaa gctcaacctc ttgtttcttg
     2881 gcgttattca ggacaggtac gcatatcaca acgagtaaca aaaacgcaaa g
//
LOCUS       DAPHDW010000245         2863 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.250, whole genome shotgun sequence.
ACCESSION   DAPHDW010000245 DAPHDW010000000
VERSION     DAPHDW010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2863
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.250"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            74..706
                     /gene="comR"
                     /locus_tag="RH057_004409"
     CDS             74..706
                     /gene="comR"
                     /locus_tag="RH057_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HDV3130660.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(768..1307)
                     /locus_tag="RH057_004410"
     CDS             complement(768..1307)
                     /locus_tag="RH057_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HDV3130661.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(1534..2838)
                     /gene="ndh"
                     /locus_tag="RH057_004411"
     CDS             complement(1534..2838)
                     /gene="ndh"
                     /locus_tag="RH057_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HDV3130662.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGVNGKVNIAIVGGGATGVELSAELHNAVKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
BASE COUNT          688 a          785 c          699 g          691 t
ORIGIN      
        1 atctggactg gtgaacggag gtgattattt ttataataac ctttcattca cagtcaccag
       61 gtacaacggc atcatggcaa ctgactcaac acaatgtgta aaaaaaagcc gtggccgccc
      121 aaaagtgttc gacagggatg ccgcgcttga taaggccatg aaattgttct ggcaacacgg
      181 ttatgaagcg acttctcttg cggacctcgt cgaagcgacc ggagccaaag cgcccacgct
      241 atacgcggaa tttaccaaca aagaggggtt atttcgtgcc gttctcgacc gctatatcga
      301 tcgttttgcc gctaagcatg aagcacagct gttttgtgaa gagaaaagcg tggagtctgc
      361 gctggctgac tattttgctg ccatcgccaa ctgctttacc agcaaagaca ccccggcagg
      421 ctgcttcatg atcaacaact gcaccaccct ctccccagat tcaggagata tcgccaatac
      481 tttgaaatca cgccatgcga tgcaagagcg cactttgcag cagtttttat gtcaacgaca
      541 agcgcgcggg gaaatcccga cccactgtga cgtgacacat ctggcagaat tccttaattg
      601 tattattcag gggatgtcga tcagcgcacg cgaaggtgca tcgctggaaa aactaatgca
      661 gattgccgga acgactttgc gtttatggcc cgaactggtg aaataagtcg caaaaaaagc
      721 ccctcagcct gagcgaatga ggggccaaat tgcagagtac agccttgtta tactttgtta
      781 ttcaaaatca gttgcccatt actatctagc gggatctgcg tacccggatc gcggtccatg
      841 cggattttgc cctgctgatc gccaatctta taggtcacat cataaccgag cattttttct
      901 gacttgtcat acaccgtttt acaacgctgt tgcgtagtcg tgtaagtatc gctttcctgg
      961 agagagccct ggatctggtt accggcatat ccaccaccca gcgcccccac aacagtggcg
     1021 acatctttac cacgaccacc accaaactga tgccctatca cgccgccagc aacagcgccg
     1081 agcaccgacc cggtaatgcg attttcatcc tgcaccggtc gacgatgggt cactgtgacg
     1141 ttgcgacact cctgacgcgg tgttttaacc gtttccttga ttggggttgc agaaacaacc
     1201 tgagcgtatt gcgggccccg ttcaaacacg ttcagactgg ccactgccgc tacgcccagc
     1261 gcagctgcga caccaatccc gatacccgcc aacattgatt tattcacggg aacacctcct
     1321 tctttgcagt tatgccaatc atgcaagcga aggagaaaag cagctatcag acagaagatg
     1381 aaaagcgcgg gaatggctgg aaatacttaa ggattcggag gagtctgaag gaatgcctga
     1441 tgcgacgctt aacgcgtctt atcaggccta ccgctcgtac catttgtcca gggcggatga
     1501 ggcgtttatg ccacatccgc cagtgtacgt cgattaatgc aacttcaaac gcggacggat
     1561 aacgcggtta atactcccca ccagcatcat taatccggtt ttaaagtaac catgcagcgc
     1621 aatctgatgc attcggtata gcgagatata tacaaagcgc gcaattcgtc cttcaatcat
     1681 cattgagccg cgcgtcaggt tacccatcag gctaccgacg gtggagaagt tcgacagcga
     1741 taccagcgaa ccatgatctt tatactgata atttttcagc ggcttaccgt tcatctgcgc
     1801 cagtatgttg ttcattgcgc aagtcgccat ctggtgtgca gcctgagcac gcggcggaac
     1861 aaagccccct tccggacgcg ggcatgacgc gcagtcgcca atagcgtaaa tgtctggatc
     1921 gcgggtggtt tgcagcgtcg gttccaccac cagctggttg atacggttag tttcaagacc
     1981 accgatatct ttcaggaagt ctggcgcttt gatcccggct gcccacacca tcagatcagc
     2041 ctcaatatat tcgccatctt tagtgtgcag gccgccttca tcagcactgg tgaccatggt
     2101 ttgcgtcagc acgcgaacgc caagtttcgt tagctcgttg tgggccgcag cagagatacg
     2161 tggcggtaat gcaggcaaaa tacgttctcc cgcttctacc agcgttacgt tcagggcttc
     2221 gttggtcagg cctttgtaac cgtagctgtg cagttgcttg accgcgttgt gcaattcagc
     2281 ggagagttct acacccgtcg cgccgccgcc gacaatcgca atgttcactt tgccatttac
     2341 gcccaggttg gcggagtatt tcaggaagag attcagcatc tcctggtgaa agcgacgcgc
     2401 ctggtgcggg ttatcgagga aaatgcagtt ctctttgaca cctggcgtat tgaaatcgtt
     2461 agaggtgcta cccagcgcca ttaccagggt gtcataggcg attttacgtt ccggaaccag
     2521 cagttcacct ttctcatcgc gcagttctgc aatagtgatt gttttcgctt cacgatcaat
     2581 atcaatgacg gaacccagct ggaactggaa accatgattg cgcgcatggg ccagatagct
     2641 caacgcatcg acgccttcat caagcgagcc agtcgccact tcgtgcagca gcggtttcca
     2701 caggtggctg tggttacgat cgaccagcgt aattttggct tttttcttac gtcccagctt
     2761 atgccccagc tgtgttgcca tttccagccc accagcaccg ccgccgacaa tcacaatctt
     2821 tttcaatggc gtagtcaacg tgaccccctt aaatttatta acc
//
LOCUS       DAPHDW010000246         2833 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.251, whole genome shotgun sequence.
ACCESSION   DAPHDW010000246 DAPHDW010000000
VERSION     DAPHDW010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.251"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            249..1415
                     /gene="nhaA"
                     /locus_tag="RH057_004412"
     CDS             249..1415
                     /gene="nhaA"
                     /locus_tag="RH057_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HDV3130663.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMMMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLALKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMAVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            1481..2380
                     /gene="nhaR"
                     /locus_tag="RH057_004413"
     CDS             1481..2380
                     /gene="nhaR"
                     /locus_tag="RH057_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HDV3130664.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAAR"
     gene            complement(2419..2833)
                     /locus_tag="RH057_004414"
                     /pseudo
     CDS             complement(2419..2833)
                     /locus_tag="RH057_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          673 a          630 c          708 g          822 t
ORIGIN      
        1 acaagaaatt aattattact tttcctaatt aatccctcag aaatcctgac cttaagctat
       61 gattatcagg gcttagggtc acccgttagc gcttacagcc gtcaaaaact catctcaccg
      121 ctgatggcgc aaatccttta attgctcgta aaaaacgagt tattcctaca ctataatctg
      181 attttaacga tgattcgtgc ggggtaaaat agtaaaaacg atctattcac ctgaaagaga
      241 aataaaaagt gaaacatctg catcgattct ttagcagtga tgcctcggga ggcattattc
      301 ttatcattgc cgctatcctg gcgatgatga tggccaacag cggcgcaacc agtggatggt
      361 atcacgactt tctggagacg ccggttcagc tccgggttgg ttcactcgaa atcaacaaaa
      421 acatgctgtt atggataaat gacgcgctga tggcggtatt tttcctgtta gtcggtctgg
      481 aagttaaacg tgaactgatg caaggatcgc tagccagctt acgccaggcc gcatttccgg
      541 ttatcgccgc tattggtggg atgattgttc cggcattact ctatctggcc tttaactatg
      601 ccgatccgat tacccgcgaa gggtgggcga tcccggcggc tactgacatt gcctttgcac
      661 ttggtgtgct ggcgctgttg ggaagtcgtg ttccgttagc gctgaagatc tttttgatgg
      721 ctctggctat tatcgacgat cttggggcca tcattatcat cgcattgttc tacactaatg
      781 acttatcgat ggcctctctt ggcgtcgcgg ctgtagcaat tgcggtactc gcggtattga
      841 atctgtgtgg tgtacgccgc acgggcgtct atattctggt tggcgtagtg ttgtggactg
      901 cggtgttgaa atcgggggtt cacgcaaccc tggcgggggt aattgtcggc ttctttattc
      961 ctttgaaaga gaagcatggg cgttctccag cgaagcgact ggagcatgtg ttacacccgt
     1021 gggtggcgta tctgattttg ccgctgtttg catttgctaa tgctggcgtt tcactgcaag
     1081 gtgtcacgct ggatggcttg acctccattc tgccattggg gatcatcgct ggcttgctga
     1141 ttggcaaacc gctggggatt agtctgttct gctggttggc gctgcgtttg aaactggcgc
     1201 atctgcctga gggaacgact tatcagcaaa ttatggcggt ggggatcctg tgcggtatcg
     1261 gttttactat gtctatcttt attgccagcc tggcctttgg tagcgtagat ccagaactga
     1321 ttaactgggc gaaactcggt atcctggtcg gttctatctc ttcggcggta attggataca
     1381 gctggttacg cgttcgtttg cgtccatcag tttgacagga cggtttaccg gggagccata
     1441 aacggctccc ttttcattgt tatcagggag agaaatgagc atgtctcata tcaattacaa
     1501 ccacttgtat tacttctggc atgtctataa agaaggttcc gtggttggcg cagcggaggc
     1561 gctttattta actccacaaa ccattaccgg acagattcga gcgctggaag agcgcctgca
     1621 aggcaaatta tttaaacgca agggacgtgg tctcgaaccc agcgagctgg gagaactggt
     1681 ctatcgctat gccgataaaa tgttcacctt aagccaggaa atgctggata ttgtgaacta
     1741 tcgcaaagaa tccaatttat tgtttgacgt tggtgtggct gatgcacttt ccaaacgcct
     1801 ggtcagtagc gtacttaacg ccgcagtggt agaaggcgag cccattcatc ttcgctgctt
     1861 cgaatccacc cacgaaatgc tgctggagca attaagtcag cataaactgg atatgatcat
     1921 ttctgactgt ccgatagact ctacgcagca ggaaggcctg ttctccgtga gaattggcga
     1981 atgtggcgtg agtttctggt gtacaaatcc accaccagaa aaaccgttcc cggcttgtct
     2041 ggaagaacgg cgacttttga ttcctgggcg acgttcaatg ttagggcgca aattgcttaa
     2101 ctggtttaac tcccagggat taaacgtaga aatcctcggc gagtttgatg atgccgcgtt
     2161 gatgaaagct tttggtgcga tgcacaatgc aatcttcgtt gccccaacgc tttatgcata
     2221 tgacttttat gccgataaaa ctgtcgtaga aattggtcgc gtcgagaatg tgatggaaga
     2281 gtaccatgct atttttgctg agcggatgat tcagcacccg gcggtacagc gaatctgcaa
     2341 tacggattat tctgcgcttt ttagtccagc ggcgcgttaa tcggcacctc cccccaaagt
     2401 taaggtgggg gagatagatt agttgtacat aaccacgatt ttgactcggc tcattatttg
     2461 cccgcttgag acattatttc catatgtacg cgggcgaata aatagaggaa tctgattact
     2521 tccttcatgg ggatgctgaa aagagtagta attgctttta ccatactgga tttcattcgt
     2581 accatcattg gagagttaaa agcctacttg acccgcaccg tcctcactgt tttctaactc
     2641 attttttaat atgctggttc ctgactcctg tatcgtcact ggttcaaagt aatattcaac
     2701 atcactgggt tcaaaaacta attcaccttg ctcgccctgg atgcagtcaa atcttatatg
     2761 atgaaactga taattttcat tattgatacg agacagtaca aatcgtccag tgtcgatgga
     2821 ttgatcttca aaa
//
LOCUS       DAPHDW010000247         2792 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.252, whole genome shotgun sequence.
ACCESSION   DAPHDW010000247 DAPHDW010000000
VERSION     DAPHDW010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2792
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.252"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            3..1967
                     /locus_tag="RH057_004415"
     CDS             3..1967
                     /locus_tag="RH057_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent
                     hemoglobin/transferrin/lactoferrin family receptor"
                     /protein_id="HDV3130665.1"
                     /translation="MNVIRNVICTLIILPIGLQAETSHSSMAKDTITVVATGNQNTVF
                     ETPSMVSVVTNDTPWSQNAVTSAGMLKGVAGLSQTGAGRTNGQTFNLRGYDKSGVLVL
                     VDGIRQLSDMAKSSGTYLDPALVKRIEVVRGPNSSLYGSGGLGGVVDFRTADAADFLP
                     PGETNGLSLWGNIASGDHSTGSGLTWFGKTGKTDALLSVIMRKRGNIYQSDGERAPNK
                     EKPAALFAKGSVGITDSNKAGASLRLYRNNTTEPGNPTQTHGDSGLRDRKTVQNDVQF
                     WYQYAPVDNSLINVKSTLYLSDITIKTNGHNKTAEWRNNRTSGVNVVNRSHTLIFPGA
                     HQLSYGAEYYRQQQKPEGSATLYPEGNIDFTSLYFQDEMTMKSYPVNIIVGSRYDRYK
                     SFNPRAGELKAERLSPRAAISVSPTDWLMMYGSISSAFRAPTMAEMYRDDVHFYRKGK
                     PNYWVPNLNLKPENNITREIGAGIQLDGLLTGNDRLQLKGGYFGTDARNYIATRVDMK
                     RMRSYSYNVSRARIWGWDMQGNYQSDYFDWMLSYNRTESMDASSREWLGSGNPDTLIS
                     DISIPVGHGGVSAGWRAELSAAATHVKKGDPHQAGYAVHSFSLSYKPVSVKGFEASVT
                     LDNAFNKLAMNGKGVPLSGRTVSLYTRYQW"
     gene            1974..2765
                     /locus_tag="RH057_004416"
     CDS             1974..2765
                     /locus_tag="RH057_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4198 domain-containing protein"
                     /protein_id="HDV3130666.1"
                     /translation="MMKNTGYILALCLTASGHVLAHDVWITGKQAENNITAEIGYGHN
                     FPSKGTIPDRRNFFENPRIYNGKETITLKPASTDYVYKTESASKDNGYVLSTYMKPGY
                     WSRTSSGWKPVSREGRNDVAYCEFVTKYAKSFIPGVQQMPAQLYQSPTGHELEIIPLS
                     DISRFSEDVKLQVLYKTSPLAGAIMELDSVSYLKSSRHTHAVEHKHPVHKAELTFVTN
                     EDGIVTVPSLHIGQWLAKVQNKKSFQDKSLCDETVDVATLSFSRN"
BASE COUNT          796 a          649 c          672 g          675 t
ORIGIN      
        1 atatgaacgt aatcagaaac gtcatttgta cattaatcat actcccgata gggttacagg
       61 cagagaccag tcattcttct atggctaaag atacaatcac cgttgtcgcg acaggaaatc
      121 agaacacggt atttgaaacg ccgtcaatgg tcagtgtcgt cacgaatgac acgccatgga
      181 gtcagaatgc ggtcacatca gccggcatgc tgaaaggtgt tgccggtctc agccagactg
      241 gtgcaggacg gaccaatggg cagaccttta atctacgcgg ctatgacaaa agcggggtac
      301 ttgttcttgt tgacggcatc cgtcaactca gtgacatggc aaaaagcagt ggcacttatc
      361 tggatccggc actcgtcaaa cgtatcgaag ttgtccgcgg accaaactcc agtctgtacg
      421 gcagtggcgg gctgggaggt gttgtggact tcagaactgc cgatgcagca gatttccttc
      481 cccccggaga gacaaacggt ttaagtctgt ggggaaatat cgccagtggt gaccacagca
      541 caggctcggg gctcacctgg ttcggtaaaa ccggaaaaac agatgcgctc ctttctgtca
      601 ttatgcgtaa aagaggtaat atctatcaaa gtgatggtga gcgcgcacct aacaaggaaa
      661 aacctgcagc cctgtttgcg aaaggctctg tcggtataac agacagtaac aaagcaggtg
      721 ccagcttgcg tctctaccgg aataacacca ctgaaccggg caatcccact cagacacatg
      781 gtgacagcgg cctgcgtgac agaaaaacag tacaaaatga cgtacagttc tggtaccagt
      841 acgctcctgt ggataacagc ctcatcaatg taaagtcaac tttatatctc agtgatatca
      901 ctatcaagac aaacggtcac aacaaaacgg cagaatggag aaacaacaga acctccggtg
      961 ttaatgttgt caacaggagt catactctga tttttccggg agcccatcag ttaagttatg
     1021 gcgctgaata ttaccgtcag cagcagaagc cagaaggctc tgccacacta tatccggaag
     1081 gaaacattga ctttacatcg ttgtatttcc aggatgaaat gacaatgaaa agctacccgg
     1141 ttaacattat cgtcggttcc cgctatgacc ggtacaagag cttcaatccc cgtgccggag
     1201 aactgaaagc cgaacgcctg tccccaaggg cggcgatttc agtctcaccg acagactggc
     1261 tgatgatgta cggctccata tcctctgcat tccgtgcccc cacaatggca gaaatgtaca
     1321 gggatgatgt acatttttac cgcaagggta aacccaatta ctgggtacct aaccttaatc
     1381 tgaaaccaga aaataacatt acccgtgaga ttggcgcagg tattcaactg gacggcctgc
     1441 ttacaggcaa tgatcggctg cagttgaaag gcggatattt cggaacggat gccagaaact
     1501 atattgccac gcgcgtggat atgaaacgga tgcgttctta ttcttataat gtatcccggg
     1561 cccgtatctg gggatgggat atgcagggaa attaccagtc tgattatttt gactggatgc
     1621 tttcttataa ccgcacggaa agtatggatg ccagcagcag ggaatggctg ggctccggca
     1681 atcctgacac actcatcagt gacatcagta tacctgttgg tcatggagga gtttctgccg
     1741 gatggcgtgc tgaactttcg gcagcagcca cgcatgtgaa aaaaggcgat ccccatcagg
     1801 ctggctatgc cgtacattcc ttttcactgt cttataagcc tgtaagtgtt aaaggctttg
     1861 aggcatcagt gactctggat aatgccttca acaagcttgc catgaatggc aaaggagtgc
     1921 cgctttcagg cagaactgtc agtctttata cccgttatca gtggtaacaa atcatgatga
     1981 aaaatacagg ttatatctta gctctttgtc tgacagcatc ggggcatgtc ctggcccacg
     2041 atgtctggat tacaggtaaa caggcagaga acaacattac cgcagagatt ggttatggtc
     2101 ataatttccc ctcaaagggc acaattcctg acagaaggaa tttctttgaa aatccccgga
     2161 tttataacgg gaaagagaca ataacactga agccagcgtc cacggattat gtctataaaa
     2221 ctgagtctgc aagcaaagat aatggctacg ttttgtcaac atatatgaaa ccgggatact
     2281 ggtcgagaac ctcttcagga tggaaaccgg tcagccggga gggcagaaat gatgtggctt
     2341 actgtgagtt tgtcactaaa tatgcgaaat cttttattcc tggtgtacag cagatgccag
     2401 cacaactcta tcagtctcca acggggcatg agcttgaaat cattccgtta tccgatataa
     2461 gtcgtttcag tgaagatgtg aagctgcaag tcctgtataa aacgtccccg ctcgccggag
     2521 ctatcatgga gcttgactcg gtcagttatc tgaaatcatc ccgtcatact catgcagttg
     2581 agcacaaaca tcctgttcat aaagcagaac tcacctttgt aactaatgag gatggtatcg
     2641 tcacagtacc ttctcttcat attggacagt ggctggcgaa agtccaaaat aagaaaagtt
     2701 ttcaggacaa aagcctgtgt gatgaaactg tcgatgtggc aactttaagt ttctcccgaa
     2761 attaacaata ctattccctg ccctgaaatt gt
//
LOCUS       DAPHDW010000248         2775 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.253, whole genome shotgun sequence.
ACCESSION   DAPHDW010000248 DAPHDW010000000
VERSION     DAPHDW010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2775)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2775)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2775
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.253"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            79..663
                     /locus_tag="RH057_004417"
     CDS             79..663
                     /locus_tag="RH057_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3130667.1"
                     /translation="MSKKLGFALSGLMLAMVAGTASADMDGGQLNISGLVVDNTCETR
                     VDGGNKDGLILLQTATVGEIDAGVLNDTVGAKAKPFSITVDCSKANPNPGSTAKMTFG
                     SVFFGNSKGTLNNDMSINNPSDGVNIALHNIDGSTIKQVQINNPGDVYTKALDATTKS
                     AVYDFKASYVRAVADQTATAGYVKTNTAYTITYQ"
     gene            768..1508
                     /locus_tag="RH057_004418"
     CDS             768..1508
                     /locus_tag="RH057_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HDV3130668.1"
                     /translation="MFFNTKHTTALCFVTCMAFSSSSIADIVISGTRVIYKSDQKSVN
                     VRLENKGNNPLLVQSWLDTGDDNAEPGSITVPFTATPPVSRIDAKRGQTIKLMYTAST
                     SLPKDRESVFWFNVLEVPPKPDAEKVANQSLLQLAFRTRIKLFYRPDGLKGNPSEAPL
                     ALKWFWSGSEGKASLRVTNPTPYYVSFSSGDLEASGKRYPIDVKMIAPFSDEVMKVTG
                     LNGKASSAKVHFYAINDFGGAIEGNASL"
     gene            1543..>2775
                     /gene="htrE"
                     /locus_tag="RH057_004419"
     CDS             1543..>2775
                     /gene="htrE"
                     /locus_tag="RH057_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Yad fimbria usher protein HtrE"
                     /protein_id="HDV3130669.1"
                     /translation="MTIKPTKNYHNHLTRIATFCALLYCNSAFCAELVEYDHTFLMGQ
                     NASNIDLSRYSEGNPAIPGMYDVSVYVNDQPIINQSITFIEIEGKKNAQACITLKNLL
                     QFHINSPDINNEKAVLLARDETLGNCLNLTEIIPQASVRYDVNEQRLDIDVPQAWVMK
                     NYQNYVDPSLWENGINAAMLSYNLNGYHSETPGRRNDSIYAAFNGGMNLGAWRLRASG
                     NYNWMTDSGSNYDFKNRYIQRDIASLRSQLILGESYTTGETFDSVSIRGIRLYSDSRM
                     LPPTLASFAPIIHGVANTNAKVTITQGGYKIYETTVPPGAFVIDDLSPSGYGSDLIVT
                     VEESDGSKRTFSQPFSSVVQMLRPGVGRWDISGGQVLKDDIQDEPNLFQASYYYGLNN
                     YLTGYTGIQITDNNYTAGL"
BASE COUNT          790 a          582 c          600 g          803 t
ORIGIN      
        1 tgcttataac atttgtgtca tgaatagtca ttcttcaaac cagtagtctg actaatggaa
       61 ttttattcaa ggatttttat gtctaaaaaa ttaggttttg ccctgtctgg tcttatgctg
      121 gcaatggttg caggtactgc ttctgctgat atggatggtg gtcagttaaa tatcagtggt
      181 ctggttgttg ataacacctg tgaaacccgc gttgacggcg gtaataaaga tggtctgatc
      241 ctgctgcaaa ctgcaactgt tggtgaaatc gatgctggcg tgctgaatga caccgttggc
      301 gctaaagcca aacctttcag catcaccgtt gactgcagca aagcaaatcc gaatccaggt
      361 agtacagcta aaatgacctt cggttctgtt ttctttggta atagcaaagg aacgctgaac
      421 aacgacatgt ctattaacaa cccgtctgac ggcgtgaata tcgctctgca caatattgat
      481 ggttccacta tcaaacaggt tcaaattaac aaccctggcg atgtgtatac taaagccctg
      541 gatgcaacga caaaatctgc tgtttatgat tttaaagcgt cttacgttcg tgccgtcgca
      601 gaccaaacag caactgctgg ttatgtaaaa actaacactg catacaccat tacttatcag
      661 taagtattca ccaattaaat tgattgaatg aatatacagg gaatagtgtt aataatttct
      721 atttttatat tattccctgt tttaattaac tctgtcaggg atggtttatg ttttttaaca
      781 ccaaacatac aacagcttta tgcttcgtaa cctgtatggc ttttagttca tcgtctattg
      841 cggacattgt catttcgggt actcgcgtaa tatataaaag cgatcaaaaa agtgtcaacg
      901 tacgtctgga aaataaaggg aataacccgt tgcttgtcca gagttggtta gatactggcg
      961 atgacaacgc tgagcctggc agtattacag tcccttttac tgccacgccg ccagtatcgc
     1021 gtattgatgc caaacgtggg caaacaatca aattaatgta cacagccagc acctcactgc
     1081 ctaaagacag agagagcgtg ttctggttta acgtactgga agttccacca aaaccagatg
     1141 cagaaaaggt cgcgaatcaa agtctgctgc aactggcatt tcgcacacgt ataaaacttt
     1201 tctatcgccc ggatggattg aagggaaatc cctctgaagc cccgttagcc ctgaagtggt
     1261 tctggtcagg ttcagaaggt aaggcgtcat tgcgagtgac caatccaacc ccttactacg
     1321 tctcttttag cagtggtgat ttagaagcta gcggtaaacg ctatccgatt gatgtgaaaa
     1381 tgattgcacc atttagtgat gaggtcatga aagtcactgg ccttaatggc aaagcaagtt
     1441 ctgcaaaagt acatttttac gccattaatg attttggtgg cgccattgaa ggtaatgcca
     1501 gtctgtaatc aggcaaggat ataattccgc aggaagcata acgtgactat aaaaccaact
     1561 aaaaattatc ataatcatct gacccgtatc gctacgtttt gcgcgctgct gtattgcaat
     1621 agtgctttct gtgctgaact tgttgaatat gaccatacct tcctgatggg gcagaatgcc
     1681 tcaaatattg atctcagccg gtatagtgaa ggtaaccctg ctataccggg tatgtacgac
     1741 gtcagtgttt atgtcaacga tcaacctatt attaaccaaa gcattacatt tattgaaatt
     1801 gaaggaaaaa aaaacgcaca agcctgtatc acacttaaga atttattgca gtttcatata
     1861 aattctcccg atataaataa cgaaaaagcc gttctgcttg ccagggatga aacactcggc
     1921 aattgcctta atttgacgga aattatccct caggcttctg ttcgttatga cgttaacgag
     1981 caacgtctgg atatagacgt tcctcaggca tgggtaatga aaaattacca aaactatgtt
     2041 gatccatcgt tatgggaaaa tggcattaat gcggccatgt tgtcatacaa cctcaacgga
     2101 tatcatagtg aaactcctgg tcgaagaaat gacagcattt atgctgcttt taacggtggg
     2161 atgaatttag gagcatggcg actgcgtgcc tcaggcaact acaactggat gaccgattct
     2221 ggcagtaatt atgattttaa gaatcggtat atacagcgtg atatcgcctc gctgcgttct
     2281 caactcattc ttggtgagtc ttatacaacg ggcgaaacct ttgattctgt cagtatccgc
     2341 ggcattcgtt tatacagcga cagccgcatg ttgcctccga cattagcgag ttttgcgcct
     2401 atcattcatg gcgttgctaa taccaacgcc aaagtcacta ttacgcaagg tggctataag
     2461 atttatgaaa cgactgtgcc gccaggcgct ttcgtcattg atgatctgag tccgtcaggt
     2521 tacggcagcg atcttattgt tactgtcgaa gaatccgatg gctcaaagcg gacattctcg
     2581 caaccttttt catcggtcgt tcaaatgtta cgccctggcg ttggacgttg ggatattagc
     2641 ggcggccagg tcttaaaaga tgatattcag gatgagccta atttatttca ggccagctac
     2701 tactacggtc tgaataacta tctaacgggt tatactggta ttcagattac cgataataac
     2761 tataccgctg gtttg
//
LOCUS       DAPHDW010000249         2751 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.254, whole genome shotgun sequence.
ACCESSION   DAPHDW010000249 DAPHDW010000000
VERSION     DAPHDW010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.254"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(97..846)
                     /locus_tag="RH057_004420"
     CDS             complement(97..846)
                     /locus_tag="RH057_004420"
                     /inference="COORDINATES: protein motif:HMM:NF024784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HDV3130670.1"
                     /translation="MDHYCTVRDTKPLDRIKLYDHYTFDPTIPQLLRVKTPYGQSKRA
                     TGEPVSFYVHQNGYASVSVLGKRYRRSRVIYSLCKGHIPEGMFVDHIDGNRLNDHPDN
                     LRLVTPQQNQWNRDNLGGKLQNYLPKGIDFVKAEGRYRARIKIDGKVRARLSYEVREL
                     MVWLQEQRQKLHGEFAYDGWTDWTEADQKRADERYEVSKPEGYKPRKRRLPGYAKTHD
                     GLKVYIEPKTGRLYFKKLNKKHYIDEESVVV"
     gene            905..1927
                     /gene="istA"
                     /locus_tag="RH057_004421"
     CDS             905..1927
                     /gene="istA"
                     /locus_tag="RH057_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100 family transposase"
                     /protein_id="HDV3130671.1"
                     /translation="MVTFETVMEIKILHKQGMSSRAIARELGISRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPATVIAREIRDQGYRGGMTILRAFIR
                     SLSVPQEQEPAVRFETEPGRQMQVDWGTMRNGRSPLHVFVAVLGYSRMLYIEFTDNMR
                     YDTLETCHRNAFRFFGGVPREVLYDNMKTVVLQRDAYQTGQHRFHPSLWQFGKEMGFS
                     PRLCRPFRAQTKGKVERMVQYTRNSFYIPLMTRLRPMGITVDVETANRHGLRWLHDVA
                     NQRKHETIQARPCDRWLEEQQSMLALPPEKKEYDVHLDENLVNFDKHPLHHPLSIYDS
                     FCRGVA"
     gene            1927..2706
                     /gene="istB"
                     /locus_tag="RH057_004422"
     CDS             1927..2706
                     /gene="istB"
                     /locus_tag="RH057_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HDV3130672.1"
                     /translation="MMELQHQRLMVLAGQLQLESLISAAPALSQQAVDQEWSYMDFLE
                     HLLHEEKLARHQRKQAMYTRMAAFPAVKTFEEYDFTFATGAPQKQLQSLRSLSFIERN
                     ENIVLLGPSGVGKTHLAIAMGYEAVRAGIKVRFTTAADLLLQLSTAQRQGRYKTTLQR
                     GVMAPRLLIIDEIGYLPFSQEEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDA
                     ALTSAMLDRILHHSHVVQIKGESYRLRQKRKAGVIAEANPE"
BASE COUNT          682 a          711 c          698 g          660 t
ORIGIN      
        1 gtgtctgcca ttttccctat actgataatt tagcaccctc ctctacagac cagacgcacc
       61 aaggcctaca gaggcgctct aagttagcaa ccgtcatcaa accaccacag actcttcgtc
      121 gatgtagtgc ttcttgttca gcttcttaaa gtagagacgc ccagttttag gctcaatgta
      181 gactttcagt ccatcatgcg tcttagcgta gccgggtaaa cgtcgcttcc ttggtttata
      241 cccttcaggc ttggacacct cgtagcgctc gtcagctcgc ttctggtctg cctctgtcca
      301 gtcggtccac ccatcgtagg cgaattcacc gtgtagcttc tgacgctgtt cctgtagcca
      361 gaccataagc tccctgacct cataagagag tctcgcccgg accttgccgt ctatcttaat
      421 acgcgctctg tagcgaccct cagcttttac aaagtcaatc cctttcggca aatagttctg
      481 aagtttgcct ccaaggttgt ctctgttcca ctggttctgc tgtggtgtaa ctaggcgcag
      541 gttgtcagga tggtcattga gtcggttacc gtcgatatgg tcaacgaaca ttccctcagg
      601 gatgtgaccc ttacacaggc tgtagatgac tctggagcga cgatagcgct tacccagtac
      661 agagacagag gcatagccat tttgatgcac atagaagcta acaggctctc ctgtggcgcg
      721 tttagattgg ccgtaaggtg tcttgactcg gagtagctga ggaatggttg ggtcaaaggt
      781 gtaatggtcg tacagtttga ttcggtctag tggcttggtg tcacgaacgg tgcaatagtg
      841 atccacaccc aacgcctgaa atcagatcca gggggtaatc tgctctcctg attcaggaga
      901 gcttatggtc acttttgaga cagttatgga aattaaaatc ctgcacaagc agggaatgag
      961 tagccgggcg attgccagag aactggggat ctcccgcaat acggttaaac gttatttgca
     1021 ggcaaaatct gagccgccaa aatatacgcc gcgacctgct gttgcttcac tcctggatga
     1081 ataccgggat tatattcgtc aacgcatcgc cgatgctcat ccttacaaaa tcccggcaac
     1141 ggtaatcgct cgagagatca gagaccaggg atatcgtggc ggaatgacca ttctcagggc
     1201 attcattcgt tctctctcgg ttcctcagga gcaggagcct gccgttcggt tcgaaactga
     1261 acccggacga cagatgcagg ttgactgggg cactatgcgt aatggtcgct caccgcttca
     1321 cgtgttcgtt gctgttctcg gatacagcag aatgctgtac atcgaattca ctgacaatat
     1381 gcgttatgac acgctggaga cctgccatcg taatgcgttc cgcttctttg gtggtgtgcc
     1441 gcgcgaagtg ttgtatgaca atatgaaaac tgtggttctg caacgtgacg catatcagac
     1501 cggtcagcac cggttccatc cttcgttgtg gcagttcggc aaggagatgg gcttctctcc
     1561 ccgactgtgt cgccccttca gggcacagac taaaggtaag gtggaacgga tggtgcagta
     1621 cacccgtaac agtttttaca tcccactaat gactcgcctg cgcccgatgg ggatcactgt
     1681 cgatgttgaa acagccaacc gccacggtct gcgctggctg cacgatgtcg ctaaccaacg
     1741 aaagcatgaa acaatccagg cacgtccctg cgatcgctgg ctcgaagagc agcagtccat
     1801 gctggcactg cctccggaga aaaaagagta tgacgtgcat cttgatgaaa atctggtgaa
     1861 cttcgacaaa caccccctgc atcatccact ctccatctac gactcattct gcagaggagt
     1921 ggcgtgatga tggaactgca acatcaacga ctgatggtgc tcgccgggca gttgcaactg
     1981 gaaagcctta taagcgcagc gcctgcgctg tcacaacagg cagtagacca ggaatggagt
     2041 tatatggact tcctggagca tctgcttcat gaagaaaaac tggcacgtca tcaacgtaaa
     2101 caggcgatgt atacccgaat ggcagccttc ccggcggtga aaacgttcga agagtatgac
     2161 ttcacattcg ccaccggagc accgcagaag caactccagt cgttacgctc actcagcttc
     2221 atagaacgta atgaaaatat cgtattactg gggccatcag gtgtggggaa aacccatctg
     2281 gcaatagcga tgggctatga agcagtccgt gcaggtatca aagttcgctt cacaacagca
     2341 gcagatctgt tacttcagtt atctacggca caacgtcagg gccgttataa aacgacgctt
     2401 cagcgtggag taatggcccc ccgcctgctc atcattgatg aaataggcta tctgccgttc
     2461 agtcaggaag aagcaaagct gttcttccag gtcatcgcta aacgttacga aaagagcgca
     2521 atgatcctga catccaatct gccgttcggg cagtgggatc aaacgttcgc cggtgatgca
     2581 gcactgacct cagcgatgct ggaccgtatc ttacaccact cacatgtcgt tcaaatcaaa
     2641 ggagaaagct atcgactcag acagaaacga aaggccgggg ttatagcaga agctaatcct
     2701 gagtaaaacg gtggatcaat attgggccgt tggtggagat ataagtggat c
//
LOCUS       DAPHDW010000250         2747 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.255, whole genome shotgun sequence.
ACCESSION   DAPHDW010000250 DAPHDW010000000
VERSION     DAPHDW010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.255"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..1878)
                     /locus_tag="RH057_004423"
     CDS             complement(<1..1878)
                     /locus_tag="RH057_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adhesin"
                     /protein_id="HDV3130673.1"
                     /translation="MNKIFKVIWNPATGNYTVTSETAKSRGKKSGRSKLLISALVAGG
                     MLSSFGALANAGNDNGQGVDYGSGSAGDGWVAIGKGAKANTFMNTSGSSTAVGYDAIA
                     EGQYSSAIGSKTHAIGGASMAFGVSAISEGDRSIALGASSYSLGQYSMALGRYSKALG
                     KLSIAMGDSSKAEGANAIALGNATKATEIMSIALGDTANASKAYSMALGASSVASEEN
                     AIALGRSSVASGTDSLAFGRQSLASAANAIAIGAETEAAENATAIGNNAKAKGTNSMA
                     MGFGSLADKVNTIALGNGSQALADNAIAIGQGNKADGVDAIALGNGSQSRGLNTIALG
                     TASNATGDKSLALGSNSSANGINSVALGADSIADLDNTVSVGNSSLKRKIVNVKNGAI
                     KSDSYDAINGSQLYAISDSVAKRLGGGAAVDVDDGTVTAPTYNLKNGSKNNVGAALAV
                     LDENTLQWDQTKGKYSAAHGTSSPTASVITDVADGTISASSKDAVNGSQLKATNDDVE
                     ANTANIATNTSNIATNTANIATNTTNITNLTDSVGDLQADALLWNETKKAFSAAHGQD
                     TTSKITNVKDADLTADSTDAVNGSQLKTTNDAVATNTTNIANNTSNIATNTTNISNLT
                     ET"
     gene            complement(1922..2605)
                     /locus_tag="RH057_004424"
     CDS             complement(1922..2605)
                     /locus_tag="RH057_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HDV3130674.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IDLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
BASE COUNT          645 a          638 c          607 g          857 t
ORIGIN      
        1 cgtctcagtc aggttagaga tgttggtggt gttagtggca atattggaag tgttattggc
       61 gatattggtg gtattcgtcg ccacagcatc gttggtggtt ttcagctgag agccgttaac
      121 agcatcagtg ctgtcagccg tcaggtcggc atctttaacg ttggtgattt tgctggtggt
      181 atcctggccg tgagctgcac tgaatgcctt tttagtttcg ttccagagca gggcatcagc
      241 ctgaaggtca ccaacggaat ccgtcaggtt ggtgatattg gtggtattgg tggcaatatt
      301 tgccgtattc gtggcaatgt tgctggtatt agtagcgata ttggcggtgt tggcttcgac
      361 atcgtcattg gtagctttca gttgggaacc gttaaccgca tccttactgg aggctgaaat
      421 cgtgccatcc gcaacatcgg tgattacgct ggcagttggg ctactagtac catgagcagc
      481 gctgtatttg cctttggttt ggtcccattg cagggtgttt tcatcaagta cagcgagcgc
      541 agcccctacg ttatttttgc taccattttt taaattgtag gttggtgctg taacagtacc
      601 gtcatcaaca tctactgcag cccctcctcc aagccttttt gctaccgagt cgctaatggc
      661 ataaagctgt gaaccattaa tggcatcgta actgtcagac ttgatcgcgc catttttcac
      721 attaacgatc ttgcgtttta atgaactatt gccgacagag acggtattgt ctaaatccgc
      781 aatggaatct gcgcccagcg cgacagagtt aataccgttg gcactgctat tactaccaag
      841 cgcaagactc ttatcaccag ttgcattact ggctgtgcct aaggcaatgg tgtttaagcc
      901 tctcgactgg ctaccattac ccagagcgat ggcatccacg ccatcagctt tgttgccctg
      961 gcctatggcg attgcattat ctgccagagc ctggctgcca tttcctaatg cgatagtatt
     1021 gactttatcg gcaaggcttc cgaaccccat tgccatgcta ttagtccctt ttgccttcgc
     1081 attattgcca atagcagttg cattttcagc ggcttcggtc tcagcaccta tcgcaatagc
     1141 gttcgctgcg ctggcaagtg attgtctgcc aaatgcgagg ctgtcagtac cgctagctac
     1201 actgctacgc cccagggcaa ttgcgttttc ttcagatgcg acgctacttg ctcccagcgc
     1261 cattgaatac gcttttgacg cattggcggt gtcgccaaga gcaatactca taatctcagt
     1321 agctttagtg gcatttccca gggcaatggc gtttgctcct tccgctttgg aagagtcccc
     1381 catagcaata gacaatttac ccaatgcttt tgaataacgg ccgagggcca ttgagtattg
     1441 gcccaatgaa tacgaagagg cacccagtgc tatacttcta tcgccttctg atattgcact
     1501 aaccccaaag gccattgatg caccgccaat cgcatgggtt tttgacccga tggcagagct
     1561 atattggcct tcagctatag cgtcataacc cacagcagta ctggaaccac tggtgttcat
     1621 aaaagtattt gctttcgccc ctttgcctat agcaacccag ccgtcgccag ctgatccact
     1681 accgtaatca acaccctgac cgttgtcatt cccggcattc gccaatgccc caaacgacga
     1741 caacattcca cccgcaacca gcgcagaaat taacagctta ctgcgcccag atttcttgcc
     1801 acggcttttt gccgtttcgc tggtaacagt ataattccct gtcgcagggt tccagataac
     1861 tttaaatatt ttgttcatta atgatgctcg ctattccttg ttataagcgt actaactgca
     1921 tttatttttt gcttcttttc tgccgatttt cagcagaggg aagattgtcg aactgaatat
     1981 tttccatgtt gatggctaac ttcagatatc ccaggttatt gggcgataca cccttcaggg
     2041 tcaaattaat gtcttgccat tgacctggta ataatttcaa tgtggatttc attgatgtgt
     2101 tgaaaagtaa ttgttgccac cgggcaaact ctttggcatc gacaggtttt tcaccacccc
     2161 atgtcgcatt aaaaccaatg tttgagatag gtgtacttaa taagttgacc agtgagatat
     2221 gaataacact accattctct gtggcgtcaa tggataacac tcgacccgct aaagccaccg
     2281 aaccgttgtt caggatgaaa tacttcgttt ttgctgggtt aaactcggta aaaataaaag
     2341 tattttgttg gtctttaacg gatttaatta cgtgagttaa agtctttgta ctgtttatta
     2401 tgccagtttg ttgtttttga atagtttcga gttgactctt tagctgtgct tgttgttttt
     2461 caagtagatc aattttattg ttatgttctt cttgttgttt caataaacta ctttctggct
     2521 tgccttcttc aatgtagtca catcccgtta atgcgcagag aactgaaaca gtgacagcac
     2581 tgaaaaaaat cttcttcaag ttcatgtaaa aatccttatt tcctccgacc agcgatcaat
     2641 gccggtagag ttgtccattg tattattggt gagctatgtt gctttacatt aatgacagtg
     2701 atgtgtgtcg attaaatatt gttttaagca ttcatcataa aaatgta
//
LOCUS       DAPHDW010000251         2736 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.256, whole genome shotgun sequence.
ACCESSION   DAPHDW010000251 DAPHDW010000000
VERSION     DAPHDW010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.256"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            74..2353
                     /gene="ydeP"
                     /locus_tag="RH057_004425"
     CDS             74..2353
                     /gene="ydeP"
                     /locus_tag="RH057_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HDV3130675.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRQDVIAMFDMNKP
                     EGFDCPGCAWPDPKYSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFTPPQAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQATLPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            2601..>2736
                     /gene="safA"
                     /locus_tag="RH057_004426"
     CDS             2601..>2736
                     /gene="safA"
                     /locus_tag="RH057_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HDV3130676.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLG
                     I"
BASE COUNT          723 a          637 c          669 g          707 t
ORIGIN      
        1 taatcccttt gtatatactg tgcatcatcg ctattacaaa tcctaataat tcatttccac
       61 acaggataag tagatgaaga aaaaaattga atcctaccag ggtgctgccg gtggttgggg
      121 tgctgttaaa tccgtagcga atgcagtacg taagcagatg gatatacgcc aggatgttat
      181 tgccatgttt gacatgaata agccagaggg ctttgactgt ccgggttgtg catggccaga
      241 tcctaagtac agtgcgtcat tcgacatttg tgaaaacggc gcaaaagcaa tcgcctggga
      301 agtcacggat aagcaggtaa atgcctcttt ctttgctgag aatacggttc aatcattact
      361 tacctgggga gatcacgagc ttgaggctgc gggacgtctc actcagcctt tgaaatatga
      421 tgctgtcagc gactgttaca agccattaag ctggcaacaa gctttcgacg aaattggcgc
      481 acgccttcaa agctatagtg atcccaatca ggttgaattc tatacttcgg gccgcacttc
      541 caatgaagct gcctttcttt atcagctttt tgcccgtgaa tacgggagta ataactttcc
      601 cgactgctcc aacatgtgcc atgaaccgac cagtgtgggt ttggcagcga gtatcggtgt
      661 gggtaaaggg actgtgttgc tggaagactt tgagaagtgc gatttagtca tttgcattgg
      721 gcataaccct ggtacaaacc acccccgcat gctgacttcg ttgcgcgctt tagtgaaacg
      781 gggagcgaaa atgatcgcca tcaatcctct acaggaacgt ggcctggagc gatttaccgc
      841 accgcaaaac ccgtttgaaa tgctgacgaa ctctgagact cagttggcta gtgcctacta
      901 taacgtgcgc attggtggcg atatggcgtt gctcaagggg atgatgcgcc tgttaattga
      961 gcgcgatgat gctgcaagtg ccgcaggtcg gccctcattg ctggatgacg aatttattca
     1021 aacgcatacc gtcggctttg acgagctacg ccgtgacgtt ctcaattccg agtggaaaga
     1081 tatcgaacgt atttctggat taagtcagac tcaaatcgcc gaactggctg atgcatatgc
     1141 cgctgccgaa cgaaccatta tctgttacgg aatggggatc actcagcacg aacatggtac
     1201 ccagaatgta cagcaactgg tcaatctgct gttgatgaaa ggtaacattg gcaagcctgg
     1261 tgcgggtatc tgcccactac gtggccactc taatgtacag ggcgaccgaa ccgtcggtat
     1321 caccgagaaa ccgtctgcag agtttctggc tcgcctgggt gagcgctatg gcttcacacc
     1381 acctcaagca cctggacatg ctgcaattgc cagcatgcaa gcaatatgta cagggcaggc
     1441 tcgagcattg atctgcatgg ggggcaactt tgcactggca atgccagatc gggaagcgag
     1501 cgctgtaccg ttaacgcaat tagatctggc ggtacacgta gccactaagc ttaaccgctc
     1561 tcatctgctg accgcacggc atagctatat tctgccggtt ctggggcgta gcgagattga
     1621 tatgcaaaaa agcggtgcgc aggcggtaac cgttgaggat tcaatgtcga tgattcatgc
     1681 ctcgcgtggc gtgttaaaac ccgccggtgt aatgctgaaa tcagagtgtg ctgtggtcgc
     1741 gggaatcgcc caggcaacac taccccagag cgtggtagcc tgggagtatc tggtggaaga
     1801 ttatgatcgc attcgcaatg acattgaagc tgtgctgcca gagttcgccg actataacca
     1861 gcgcatccgt catcccggtg gttttcacct gataaatgca gctgctgaaa ggcgctggat
     1921 gacgccgtca ggtaaggcta atttcattac cagcaaaggg ctgttagaag atccctcttc
     1981 agcgtttaac agtaagctgg tcatggcgac agtacgcagc cacgatcagt acaacacgac
     2041 gatttatggt atggatgatc gctatcgagg ggtattcggt caacgagatg tggtctttat
     2101 gagtgctaaa caagctaaaa tttgccgtgt aaaaaacggc gaaagagtta atcttattgc
     2161 gcttacgcca gacggtaagc gcagctcacg ccgcatggat agattaaaag tggtcattta
     2221 ccctatggct gaccgctcac tggtgaccta ttttccagaa tcgaatcaca tgctaactct
     2281 tgataaccac gatccattaa gtggcattcc tggttataaa agtattccgg ttgaattaga
     2341 accatcaaat taattcgcct cctcatttct tctgctgtca tccgcacagc agaagaattt
     2401 ctcattgact attatcgcgc aatttgctca cattgattaa attaaaccac acactataag
     2461 atataaacct ctgcctacag ctgtaagaaa ctccgctcag tactgaagtg ccagtcctat
     2521 ttcctctttt ctccagcctg ttatattaag catactgatt aacgattttt aacgttatcc
     2581 gctaaataaa catatttgaa atgcatgcga ccacagtgaa aaacaaaatc acgcaaagag
     2641 acaactataa agaaatcatg tctgtaattg tggttgtctt attactgacc cttacgttga
     2701 tagccatttt ttcggcaatt gatcagctgg gtattt
//
LOCUS       DAPHDW010000252         2733 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.257, whole genome shotgun sequence.
ACCESSION   DAPHDW010000252 DAPHDW010000000
VERSION     DAPHDW010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.257"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..92
                     /locus_tag="RH057_004427"
     CDS             <1..92
                     /locus_tag="RH057_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid-binding protein"
                     /protein_id="HDV3130677.1"
                     /translation="QYSDHANQLILITDNDSMAASVTLPWAIK"
     gene            100..786
                     /locus_tag="RH057_004428"
     CDS             100..786
                     /locus_tag="RH057_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV3130678.1"
                     /translation="MANLIRKEVTFESSIAAIGAAMSDISRVKILSALMDGRAWTATE
                     LSSVANISASTASSHLSKLLDCQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHG
                     VHAKVSTPVHLRKARTCYDHLAGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMG
                     IDVPSKHSRKICCACLDWSERRFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKG
                     YAAFKTHFHI"
     gene            complement(764..1387)
                     /gene="tetR(B)"
                     /locus_tag="RH057_004429"
     CDS             complement(764..1387)
                     /gene="tetR(B)"
                     /locus_tag="RH057_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDV3130679.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1469..2674
                     /gene="tet(B)"
                     /locus_tag="RH057_004430"
     CDS             1469..2674
                     /gene="tet(B)"
                     /locus_tag="RH057_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDV3130680.1"
                     /translation="MNSSTKIALVITLFDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          704 a          533 c          606 g          890 t
ORIGIN      
        1 tgcaatacag tgaccatgct aaccaactga tattaataac ggacaatgat agtatggctg
       61 catctgttac gctcccttgg gcaataaagt gaacttgcga tggctaattt aatacgaaaa
      121 gaggttacct ttgagtcctc aatagccgcg ataggggcgg ctatgtctga catttcacga
      181 gttaaaatac tcagtgcttt gatggatggg cgagcttgga cggccactga gctaagttct
      241 gtggcgaata tatcagcttc aacggcgagc agtcatttat ctaaattatt agattgccag
      301 ctaatcacag tagtagctca aggcaagcat cgttattttc ggctagcagg aaaagatatt
      361 gctgaattga tggaaagtat gatggggatc tccttaaacc atggcgtaca tgccaaagtt
      421 tccacgccag tgcatttacg aaaagcacgt acttgctatg atcatttagc tggcgaagtt
      481 gccgttaaga tctatgattc cctttgtcaa cagcaatgga tcactgaaaa tggttcaatg
      541 atcacattaa gtggtattca atattttcat gaaatgggaa ttgacgttcc ttccaaacat
      601 tcacgtaaaa tctgttgtgc gtgtttagat tggagtgaac gccgtttcca tttaggtggg
      661 tacgttggag ccgcattatt ttcgctttat gaatctaaag ggtggttaac tcgacatctt
      721 ggttaccgtg aagttaccat cacggaaaaa ggttatgctg cttttaagac ccactttcac
      781 atttaagttg tttttctaat ccgcatatga tcaattcaag gccgaataag aaggctggct
      841 ctgcaccttg gtgatcaaat aattcgatag cttgtcgtaa taatggcggc atactatcag
      901 tagtaggtgt ttccctttct tctttagcga cttgatgctc ttgatcttcc aatacgcaac
      961 ctaaagtaaa atgccccaca gcgctgagtg catataatgc attctctagt gaaaaacctt
     1021 gttggcataa aaaggctaat tgattttcga gagtttcata ctgtttttct gtaggccgtg
     1081 tacctaaatg tacttttgct ccatcgcgat gacttagtaa agcacatcta aaacttttag
     1141 cgttattacg taaaaaatct tgccagcttt ccccttctaa agggcaaaag tgagtatggt
     1201 gcctatctaa catctcaatg gctaaggcgt cgagcaaagc ccgcttattt tttacatgcc
     1261 aatacaatgt aggctgctct acacctagct tctgggcgag tttacgggtt gttaaacctt
     1321 cgattccgac ctcattaagc agctctaatg cgctgttaat cactttactt ttatctaatc
     1381 tagacatcat taattcctaa tttttgttga cactctatca ttgatagagt tattttacca
     1441 ctccctatca gtgatagaga aaagtgaaat gaatagttcg acaaagatcg cattggtaat
     1501 tacgttattc gatgccatgg ggattggcct tatcatgcca gtcttgccaa cgttattacg
     1561 tgaatttatt gcttcggaag atatcgctaa ccactttggc gtattgcttg cactttatgc
     1621 gttaatgcag gttatctttg ctccttggct tggaaaaatg tctgaccgat ttggtcggcg
     1681 cccagtgctg ttgttgtcat taataggcgc atcgctggat tacttattgc tggctttttc
     1741 aagtgcgctt tggatgctgt atttaggccg tttgctttca gggatcacag gagctactgg
     1801 ggctgtcgcg gcatcggtca ttgccgatac cacctcagct tctcaacgcg tgaagtggtt
     1861 cggttggtta ggggcaagtt ttgggcttgg tttaatagcg gggcctatta ttggtggttt
     1921 tgcaggagag atttcaccgc atagtccctt ttttatcgct gcgttgctaa atattgtcac
     1981 tttccttgtg gttatgtttt ggttccgtga aaccaaaaat acacgtgata atacagatac
     2041 cgaagtaggg gttgagacgc aatcgaattc ggtatacatc actttattta aaacgatgcc
     2101 cattttgttg attatttatt tttcagcgca attgataggc caaattcccg caacggtgtg
     2161 ggtgctattt accgaaaatc gttttggatg gaatagcatg atggttggct tttcattagc
     2221 gggtcttggt cttttacact cagtattcca agcctttgtg gcaggaagaa tagccactaa
     2281 atggggcgaa aaaacggcag tactgctcgg atttattgca gatagtagtg catttgcctt
     2341 tttagcgttt atatctgaag gttggttagt tttccctgtt ttaattttat tggctggtgg
     2401 tgggatcgct ttacctgcat tacagggagt gatgtctatc caaacaaaga gtcatcagca
     2461 aggtgcttta cagggattat tggtgagcct taccaatgca accggtgtta ttggcccatt
     2521 actgtttgct gttatttata atcattcact accaatttgg gatggctgga tttggattat
     2581 tggtttagcg ttttactgta ttattatcct gctatcgatg accttcatgt taacccctca
     2641 agctcagggg agtaaacagg agacaagtgc ttagttattt cgtcaccaaa tgatgttatt
     2701 ccgcgaaata taatgaccct cttgataacc caa
//
LOCUS       DAPHDW010000253         2713 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.258, whole genome shotgun sequence.
ACCESSION   DAPHDW010000253 DAPHDW010000000
VERSION     DAPHDW010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.258"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            283..1155
                     /locus_tag="RH057_004431"
     CDS             283..1155
                     /locus_tag="RH057_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV3130681.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQRVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCIASVARTVLRAVRDS
                     VVSVARAVWNWIFF"
     gene            1527..>2713
                     /gene="ag43"
                     /locus_tag="RH057_004432"
     CDS             1527..>2713
                     /gene="ag43"
                     /locus_tag="RH057_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV3130682.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKGAGVAVALSLAA
                     VTSVPALAADSIVQAGETVNGGTLENHDNQIVLGTANGMTISTGLELGPDSEENTGGQ
                     WIQNGGIAGNTTVTTNGRQVVLEGGTASDTVIRDGGGQSLNGLAVNTTLNNRGEQWVH
                     EGGVATGTIINRDGYQSVKSGGLATGTIINTGAEGGPDSDNSYTGQKVQGTAESTTIN
                     KNGRQIILFSGIARDTLIYAGGDQSVHGRALNTTLNGGYQYVHKDGLALNTVINEGGW
                     QVVKAGGAVGNTTINQNGELRVHAGGEATAVTQNTGGALVTSTAATVTGINRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAV"
BASE COUNT          654 a          711 c          760 g          588 t
ORIGIN      
        1 atacactgaa aaatactcac agcagtaaac acagacacac ctgataatca tacagccgat
       61 aaatattttc ccctttacgg agaaatcagc ctgctgttta acaataacgg cgtattttca
      121 tggaaggaga tcacccagta acggataagt cctgatctcc ttcatgttaa tacgcaacaa
      181 tcccatcgat tcaaaacaat ttcagacaga tattatctcc gtgaatcccc cgccaccttt
      241 ccggtgcgcg gggttttgtc ttttttcacc gggaatacat gtatgaatcc gtctgatgcc
      301 attgaggcaa ttgaaaaacc gctctcctcc ctgccttact cactttcccg tcacatcctg
      361 gaacatctgc gcaaactcac ccgtcacgaa cccgtgattg gcattatggg taaaagcggg
      421 gccggtaaat cctcactctg taatgcactg tttcaggggg aggtcacccc ggtcagtgat
      481 gttcacgccg gcacccggga agtgcagcgc ttccgtctga gtggccacgg tcacagcatg
      541 gttatcactg acctgcccgg ggtgggcgag agccgggaca gggatgcaga gtatgaggcc
      601 ctgtaccgtg acattctgcc tgaacttgac ctggtactgt ggctgattaa agccgatgac
      661 cgtgccctgt ctgtggatga gtatttctgg cgacacatcc tgcaccgggg acatcagcgg
      721 gtactgtttg tggtgacgca ggccgacaaa acggagccct gccatgaatg ggatatggcc
      781 ggtattcagc cttctcctgc acaggcacag aatattcgcg aaaaaacgga ggcggtattc
      841 cgtctgttcc ggcccgtaca tccggttgtg gccgtatcgg cccgcaccgg ctgggaactg
      901 gatacgctgg tcagtgcgct catgacagcg cttcccgacc atgccgccag tcccctgatg
      961 acccgactgc aggacgagct gcgcacggaa tctgtccggt ctcaggcccg tgaacagttt
     1021 accggtgcgg tggaccggat atttgacacg gcggagagcg tctgtattgc ctctgttgca
     1081 cgcacggttc tgcgtgccgt ccgtgactcg gtggtctctg ttgcccgcgc tgtatggaac
     1141 tggattttct tctgaacact ctgccagggg aaatgcctga ttatattgca ttaacaaact
     1201 gaaaacatga cacaaacccc tcataccctc ctgtttctct tgcgtgaccg ccttgctgtt
     1261 aatagaataa aacgatcgat aaaacaggta tcgatcgctg ctatcgatcg tttatagtgg
     1321 ttaatggcct gcgaaaaggc agctgaatct cttcatcatg cagaacggaa ttgcacacaa
     1381 cggactgata cttctctggc ggatgagaga ggggaagcat ttatgtccgg gaaaaaccac
     1441 tttatctgac catgctgttt tgtacctgcc ggtatccaca tttgtgggta ccggcttttt
     1501 tattcaccct ctgtaaggaa aagctgatga aacgacatct gaacaccagc tacaggctgg
     1561 tatggaatca cattacgggc accctggtgg tggcctccga actggcccgc tcacggggaa
     1621 aaggcgccgg tgtggcggtt gctctgtctc ttgctgctgt cacgtcagtc ccggccctgg
     1681 ctgctgacag tattgtacag gcaggtgaaa ccgtgaacgg cggaacactg gaaaatcatg
     1741 acaaccagat tgtcctcggt acggccaacg gaatgaccat cagtaccggg ctggaactgg
     1801 ggccggacag tgaagaaaac accggtgggc aatggataca gaatggcggg atagccggaa
     1861 acaccactgt taccacaaat ggtcgtcagg tcgtgctgga ggggggaaca gccagtgata
     1921 cggttattcg tgacggcggg ggacagagcc tgaacgggct ggcggtgaac accacgctga
     1981 ataacagagg cgagcagtgg gttcatgagg gcggggttgc caccggtaca attatcaacc
     2041 gcgacggtta ccagagcgtt aaaagtggcg ggctggcaac aggaaccatc atcaacaccg
     2101 gcgcagaagg cggcccggat tctgacaact cgtacacggg tcagaaggtc cagggaacag
     2161 cagaatccac caccatcaac aaaaatggac ggcagattat cttattttcc gggatagccc
     2221 gtgacactct catttacgca ggtggtgacc agtcggtaca cggaagggcc ctgaatacca
     2281 cactgaatgg cggttaccag tatgtgcaca aggacggact tgcgctgaac acggtaatta
     2341 acgagggggg ctggcaggtt gttaaggcag gtggtgctgt cggtaacacc accataaatc
     2401 agaacggtga actgagggta catgccggcg gggaagccac tgcagtcacc cagaacacgg
     2461 gcggtgcact ggttaccagt acggctgcaa ccgttaccgg cataaaccgc cttggagcat
     2521 tctcggttgt ggagggtaaa gctgataatg tcgtactgga aaatggcgga cgcctggatg
     2581 tgctgaccgg acacacagcc actaataccc gcgtggatga tggcggaacg ctggatgtcc
     2641 gcaacggtgg caccgccacc accgtatcca tgggaaatgg cggtgtactg ctggccgatt
     2701 ccggtgccgc tgt
//
LOCUS       DAPHDW010000254         2651 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.259, whole genome shotgun sequence.
ACCESSION   DAPHDW010000254 DAPHDW010000000
VERSION     DAPHDW010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2651
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.259"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(155..373)
                     /locus_tag="RH057_004433"
     CDS             complement(155..373)
                     /locus_tag="RH057_004433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130683.1"
                     /translation="MNNSNNLDYFTLYIIFSIAFMLITLLVILIAKPSTGLGEVLVTI
                     NLLNALVWLAINLVNRLRERLVSHRDQQ"
     gene            complement(505..2028)
                     /locus_tag="RH057_004434"
     CDS             complement(505..2028)
                     /locus_tag="RH057_004434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HDV3130684.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECNLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVFPDSNLLLAPAIDTVNRLPILLYQNQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTGDVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIACAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLDYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
     gene            complement(2360..2608)
                     /locus_tag="RH057_004435"
     CDS             complement(2360..2608)
                     /locus_tag="RH057_004435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130685.1"
                     /translation="MNNSIPERFIFQCALFKNLEREVFMTHGYVDSHIIDQALRLRLK
                     DETSVILSDLYLQILQYIEMHKTTLTDIIINDRESVLS"
BASE COUNT          820 a          570 c          543 g          718 t
ORIGIN      
        1 gttgcaataa aaattatttc aatgacatat aaaaacaact gtgataacac cagggttgac
       61 aactatttgc gcaataattc ctttacttca atgatccagg cattcactgc cagcaaccca
      121 ttcagccgca tactgacagt gagaaactga aagattattg ctgatccctg tggctgacga
      181 gtctttccct taatcgatta accagattga tcgccagcca aacaagggca ttaagcaaat
      241 ttatcgtcac aagcacttct cccagcccgg tactgggttt tgcaataagg atgaccagga
      301 gggtgatcag cataaatgca atggaaaaga tgatatagag agtgaaataa tccagattat
      361 tactattgtt catgctacta atggcaatcc tgtcaaaact ttcaaacaat tatagcaaac
      421 aaaataatga acaatagtaa caagaggcaa cgagctaatt agctgtctat aattacatcg
      481 cactatggcc ttactgatta attttcactc aaccacaacc ttcaccttcg gtttagaaag
      541 gataattttg accaggtcga tgtatgtaac aggtttatag aaataataac cctgctgaaa
      601 tgtgatatta ttttggttca gatagtcaag ttgttctttc gtttcgacac cttcagcgac
      661 gatactgatc gaaagtttac gcgccagatc caatacacaa tccagaattc gggttgattc
      721 tgggtctgcg tttacgcggc caacgaaact gtgatcaatt ttgatataat caatatgcag
      781 gtcatgaaga taagaaagcc ctgagtagcc agtaccgaaa tcatccagcg cgatgacaaa
      841 accattttca tgcagaatgt tcaaccgctg aaccagactt tcatcaacat tcaatggctc
      901 acgctcggtg acttccagaa caaggtttaa atcgcggcgg gtaaaactgt cacggaaatt
      961 taaacactcg tcgacaaacg tcggcgaaat aatatgcgaa gcgctaaaat taattccaat
     1021 atgaaaacct tccggcaatt tactcgcgat agcgttcatc tgtctggcaa cctgattcat
     1081 caggctttgc gtaagcggaa cgattaatcc ggatttttca gcaagtggaa taaatgccgc
     1141 gggtgatata tatccaccgt gaggttgttt ccagcgggct aacacctcaa ctccccgcaa
     1201 tgtcccttcc cgaccattta ccacaggttg gtaaaaaggg acgatctccc cattatttat
     1261 tgcccgtcgc aggatctctt caggcgttgc gcttttattg aaatatctat caagcaggaa
     1321 ggcagcggcg caggcgatta acagaataaa tattaaaatc ccaaagcctt tatcgataag
     1381 tctgtagaag ctaaagagtg gcggcgggtt aaagataata gtgaaatgat attttgtgga
     1441 gtgaaccttc tcggtatagg gataatgtcc attaagcgtc atcacatcac ccgttggtcc
     1501 aataatgtca tccgccacgc gtaatacata ccttacccct ttcaagggta cattaagtgc
     1561 cccacgaata tgctgatcgc ttatcgtaac caaaatgcgc gtatctgcaa attggttctg
     1621 atagagcagg ataggtaatc tattaacggt gtcgattgct ggagccaaca gtaaattact
     1681 gtccgggaaa acaggaatac gagacaacag gacccgattc ccaggcaggg atgtacacca
     1741 gactattccc tgtttgagaa tgatgattgt gcgcaggtga ggtttcagag ctgcttcagt
     1801 gccaagctga tattgctcct cgaggttgca ttccttcccg gcaatgttaa ccgctgtttg
     1861 agtcgcttgt gacgcttctt caagtatatg attgatattg ttggcggcat atctcgctcc
     1921 cgccaggtac tctgcttttg cggaatacca gagctggatg ttcaaaatag cgacaccggt
     1981 aataaacaag catatcgcaa cgaggatggg tatgagtgta ttgcgcattt gcttgtcccg
     2041 gccaaagggt ttaatacatg aaaatgccat tcattcgctc gcgctggcat ctacagagag
     2101 catggtagag agcataagaa atatccacgc cgaagataaa attttctcca tcttgatcta
     2161 acccattaat tattgagcat aatgtaggca gtacaaaata agttaggcgg gatatcaggc
     2221 gtcaagaatg gagagcgaga actctccatt cttgacacct gatattgcgg acataataag
     2281 aaagcataac gcctgaaatg ctcactttgc atcagcatgg tgatacagct gatgtttatt
     2341 ctaaaacctt actcaagttc taagagagca cggattccct gtcattaata atgatatccg
     2401 ttagtgtagt cttatgcatt tcaatgtact gcaatatttg cagatagaga tcagaaagaa
     2461 tcacactggt ttcatctttt aaacgcaagc gtaaagcctg atcaataata tgactatcaa
     2521 catacccatg cgtcatgaac acctccctct cgagattttt aaacaaagca cattgaaaaa
     2581 taaatctctc tgggattgaa ttattcatgt cagcctcgta gaaatattaa cccaataaaa
     2641 ttatgctgtg t
//
LOCUS       DAPHDW010000255         2635 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.260, whole genome shotgun sequence.
ACCESSION   DAPHDW010000255 DAPHDW010000000
VERSION     DAPHDW010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.260"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            126..284
                     /locus_tag="RH057_004436"
                     /pseudo
     CDS             126..284
                     /locus_tag="RH057_004436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323645.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            281..778
                     /locus_tag="RH057_004437"
                     /pseudo
     CDS             281..778
                     /locus_tag="RH057_004437"
                     /inference="COORDINATES: protein motif:HMM:NF024728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemagglutinin repeat-containing protein"
     gene            815..1928
                     /locus_tag="RH057_004438"
                     /pseudo
     CDS             815..1928
                     /locus_tag="RH057_004438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001531944.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VENN motif pre-toxin domain-containing protein"
     gene            2108..2608
                     /locus_tag="RH057_004439"
                     /pseudo
     CDS             2108..2608
                     /locus_tag="RH057_004439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000931688.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          724 a          593 c          797 g          521 t
ORIGIN      
        1 atcgtcaata ataatattta cagcattaaa ctccatggta attttggtgg cgaggcaggg
       61 gattaccgtt gttcattccg gcgctgatgc tgagagtttg tttccctgtc agagtaaacg
      121 gggttatgaa ttcggaggtt tgtttgcatc tggtgaaaga gcggtccggg attctgctgc
      181 ccggggaagt atcagaaagg catttctggt tattgattga aatatccggg atgcacagtg
      241 agaaagtgat taatgcgctc agggagcatc ttgttatggg atatatcagc acgatatcgt
      301 ctccggctca actctcagcg ccggtaacaa tgtctccgtc actgccacgg ggaaaaataa
      361 ggaccataac aacagcggcg atattctcat taccgggagc cagattaagt ccggcaatga
      421 caccagcctg aatgcacaga acgatattct gctggctgcc gccgctgaca cccgacagac
      481 caccgggaag aacagcagca aaggtggcgg cgtgggcgtc agctttggtg gtggtactaa
      541 tggtggcggg ctgagtatct tcgccagcat taacggctcc aaaggcagtg agaaaggcaa
      601 cggcacgacc tggaccgaaa ccacgctgga tgcgggtaaa aatgtctccc tcacaagcgg
      661 acgtgatacc accctgtcag gggctcaggt cagtggcgag aaagtcacag cagatgtcgg
      721 caacaacctg accatttcga gccttcagga cagtgaccgt tacgacagca gacagaacag
      781 cgtggccgca ggtggcagct tcacgtttgg ttcaatgtcc ggcagcggct atgccagcat
      841 cagtcaggac aaaattaaaa gcaattacga ttctgtccgg gaacagagtg gtatctacgc
      901 cggaaaagac gggtttgatg tcactgtggg gaatcacact cagctcaatg gtgcggtaat
      961 tgcgtccacg gcaacggatg acaaaaacag cctcagtacc ggcacgctgg gctggtcgga
     1021 tatccataac caggcagact ataaagccag ccataccgga atcagttcat cgggtggctc
     1081 agggatgtca gccagccaga tggtggcctc aaatgcaata gccggtgcgg cgaacgcact
     1141 taccggtatg tccggcagca gtggacatgc tgaaggtacc acgtcatccg ccatcagcgg
     1201 cggaaatatc attatccgtg ataaggagag tcagaagcag gatattgccg ggctcagccg
     1261 ggacccggaa aatgccaacg gcagcattgc accgatattt gacagggaga aagagcagaa
     1321 gcgtcttcag gaagcgcagg ttatcagcca gattagcggt cagatgagta acatcgtcat
     1381 gacgtatggc gaaactgaag cgatgaaagc agccagggcg aaatatccag ggctgagtga
     1441 tgcacagttg cgggagaccc cggaatacag agaggtcatg aaaggctacg gtacgggcag
     1501 tacgccgcag atggtggtac aggccatcac gggcgtgctg ggcggactga atgcagggaa
     1561 tccggggcag gtactggctg gcggtctgaa tccagcagtg gcacagctta taaaacaggc
     1621 taccggtgac aaccgggaag caaacctgat ggctcacgca gtctggggag cgcttgctgc
     1681 acaactgggc ggaaacaatg cggcttccgg tgctgcaggg gcattcagtg gtgaacttgc
     1741 tgcccggtat atcattgata actactacgg tggttgtacc gataacctga gtgaacagga
     1801 acgtcagcaa atcagcatgc tggtcaccat tgcctcaggt attgcgggcg ggcttgccgg
     1861 caacagtaca agtgctgccg gtacgggtgc acaggcaggt aggaactcgg ctgaaaacaa
     1921 tatgctgaac gtgatagcca ctgaaacagc agctaatacg gcagcagcaa tagctggtgc
     1981 tacaggtaat gccagtaagg ataccgggaa tgataatagc tgggataccg gtggtggttg
     2041 tgaaggctca cgggaacagt gtgctgtacg tgatccgagt cgcgatggca cccggggacc
     2101 ggggcatgtg ctgaatggcg acaatacaga cgaagataaa cagcctaacc agggtaaaga
     2161 actggctggc gcaacggaca atgagaaagc cgagcttggt agtgcaggtg ctggaacacc
     2221 gggggatggg gaccagataa tgaagaaaaa gcccgtcatc aggaggttca gcaacaacga
     2281 tttgatgaac tgagtaaaat atatgataaa agtcacccgg ttggagagtt aacggtggat
     2341 gggcagacta tcaggcagag tttggtaagt aaccgttatg gtacgacgaa agtgtttgaa
     2401 agccagaatt tgacggataa acagatccac aattacgcac agcaactggc aggcgataca
     2461 ccgctgaaag aggttcggcc ggggtgcaaa gctggataac ggaacatcga tcacgttgcg
     2521 aaatttatcg tcgtctcaag aacaaacagg tgccagatgg actattgata ttaaggtgcg
     2581 tattaccgtg aaggagatcg gtgagtaacg gcgtttttca aggtagttgt cgcct
//
LOCUS       DAPHDW010000256         2605 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.262, whole genome shotgun sequence.
ACCESSION   DAPHDW010000256 DAPHDW010000000
VERSION     DAPHDW010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.262"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            45..998
                     /gene="ompT"
                     /locus_tag="RH057_004440"
     CDS             45..998
                     /gene="ompT"
                     /locus_tag="RH057_004440"
                     /EC_number="3.4.23.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease OmpT"
                     /protein_id="HDV3130686.1"
                     /translation="MRAKLLGIVLTTPIAISSFASTETISFTPDNINADISLGTLSGK
                     TKERVYLAEEGGRKVSQLDWKFNNAAIIKGAINWDLMPQISIGAAGWTTLGSRGGNMV
                     DQDWMDSSNPGTWTDESRHPDTQLNYANEFDLNIKGWLLNEPNYRLGLMAGYQESRYS
                     FTARGGSYIYSSEEGFRDDIGSFPNGERAIGYKQRFKMPYIGLTGSYRYEDFELGGTF
                     KYSGWVEASDNDEHYDPGKRITYRSKVKDQNYYSVAVNAGYYVTPNAKVYVEGAWNRV
                     TNKKGNTSLYDHNDNTSDYSKNGAGIENYNFITTAGLKYTF"
     gene            1185..>2605
                     /locus_tag="RH057_004441"
     CDS             1185..>2605
                     /locus_tag="RH057_004441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HDV3130687.1"
                     /translation="MRKIITHFKVVLTLLLPVTVSAQQIQWQSCMASQFNHWFGEEKP
                     SPDLLCGYLSVPLKYTDTGGDASYEKKSQVKLALTKLPAKSKHKGSILIISGGPGLPG
                     INPYINFDWPVTNLRESWDIIGFDPRGVGQSTPTINCRQSDTETQENITEKQQVLNKI
                     NACIHNTGAEVIRHIGSNEAVYDIDRIRQALGDKQLTAVAYSYGTQIAALYAERFPYN
                     VRSIVLDGVVDIDDLEDNFTWQLKQAQSYQETFDRFASWCARTKSCPLSSDRDKAITQ
                     FHELLSKLHHKPLLDSKGENISSDELISLTTDLLLWRSSWPTLATAIRQFSQGIVSNE
                     IETALSAPIASEESSDASGVILCVDQGDEQLTPEERKSRKDALANAFPAINFDNGRSD
                     SPDFCELWPIHSDLNKTRLKNTVLPSGLLFVAHKYDPTTPWINARKMAEKFSSPLLTI
                     NGDGHTLALTGVNLCVDKAVVHHL"
BASE COUNT          821 a          559 c          544 g          681 t
ORIGIN      
        1 aaaatacata ttcaatcatt aaaacgattg aatggagaac ttttatgcgg gcgaaacttc
       61 tgggaatagt tctgacaacc cctattgcga tcagctcttt tgcttctacc gagactatat
      121 cgtttactcc tgacaacata aatgcggaca ttagtcttgg aactctgagc ggaaaaacaa
      181 aagagcgcgt ttatctagct gaagaaggag gccgaaaagt cagtcaactc gactggaaat
      241 tcaataacgc tgcaattatt aaaggtgcaa ttaattggga tttgatgccc cagatatcta
      301 ttggggctgc gggctggact actctcggca gccgaggtgg caatatggtc gatcaggact
      361 ggatggattc cagtaacccc ggaacctgga cagatgaaag taggcacccg gatacacaac
      421 tcaattatgc caacgaattt gatctgaata tcaaaggctg gctcctcaac gaacccaatt
      481 accgcctggg actcatggcc ggatatcagg aaagccgtta tagctttaca gccagaggtg
      541 gttcctatat ctacagttct gaggagggat tcagagatga tatcggctcc ttcccgaatg
      601 gagaaagagc aatcggctac aaacaacgtt ttaaaatgcc ctacattggc ttgactggaa
      661 gttatcgtta tgaagatttt gagctaggtg gcacatttaa atacagcggc tgggtggaag
      721 catctgataa cgatgagcac tatgacccag gaaaaagaat cacttatcgc agtaaagtca
      781 aagaccaaaa ttactattct gttgcagtca atgcaggtta ttacgtaaca cctaacgcaa
      841 aggtttatgt tgaaggcgca tggaatcggg ttacgaataa aaaaggtaat acttcacttt
      901 atgatcacaa tgataacact tcagactaca gcaaaaatgg tgcaggcata gaaaactata
      961 acttcatcac tactgctggt cttaagtaca ccttttaacg aagttaacca gattttctcc
     1021 ccggtggtat tgtataaccc cggggaattt gtttacagtt acaagtaggc aatatccact
     1081 aattttgtaa gcatttaacc tatgtcataa cgtgtaatct cttaccgcaa cacaagacct
     1141 caatatattc aaatcttatc tttattccga tattcagaga cacaatgcga aaaataatta
     1201 ctcatttcaa agttgtttta acgttacttc taccagtaac cgtatctgcc cagcagatac
     1261 agtggcaatc ctgtatggcc agtcaattca accactggtt tggtgaggaa aaaccgtctc
     1321 ctgacttact atgtggttat ttgtctgttc cattaaaata tacagacaca ggcggagatg
     1381 cttcttatga aaaaaaatca caagtcaaac tagcgttgac aaaattgccg gcaaaaagca
     1441 agcataaagg aagtatcctg ataataagtg gtggtcccgg gttaccaggc ataaatcctt
     1501 atattaactt tgactggcca gtcacaaatc ttcgtgagtc atgggatatt attggatttg
     1561 atcctcgagg cgtcggacag tccactccga caataaactg ccggcaatca gatacagaga
     1621 ctcaggaaaa cataaccgaa aagcaacaag tattaaataa aattaatgcc tgtatccata
     1681 ataccggagc cgaagtcatt cgccatatcg gctctaacga ggctgtatac gatattgatc
     1741 gtattaggca agccttgggg gataaacaac tgacagccgt ggcgtattcg tatggaactc
     1801 aaattgcagc cttatatgca gaacgttttc cctacaacgt aagatctatc gttcttgatg
     1861 gagtcgtcga tatcgatgac ctggaggaca acttcacatg gcaactcaaa caggcacaga
     1921 gttatcagga aacgtttgat cgctttgcat cctggtgtgc gcgtacaaaa agttgcccgc
     1981 tttcttcaga cagagataag gcaataactc agttccatga gctattatca aaattacatc
     2041 acaaaccttt attagacagt aagggagaaa atatatcttc agatgaactc atatcattaa
     2101 caacagacct tctgctatgg cgttcatcat ggccaaccct tgcaactgcc atacgccagt
     2161 tctctcaggg gattgtcagt aatgaaattg aaactgcgct cagtgctccg atagcctcag
     2221 aagagtcaag cgatgcttcg ggggtaatcc tctgtgtaga tcagggggat gagcaattaa
     2281 caccagaaga gcgaaaatcc cgaaaagacg ctcttgcgaa tgccttcccg gctattaact
     2341 ttgacaatgg acgttccgat tcacctgatt tttgtgaatt atggccaata catagcgacc
     2401 tgaacaaaac tcgcctgaaa aatactgttc tgccctctgg tttactgttt gtagcacaca
     2461 aatacgaccc aacaacgccc tggattaatg cccgtaagat ggcagagaaa ttttccagcc
     2521 cgttactaac aataaatggt gatgggcata cattagctct caccggagtt aatttatgtg
     2581 tagataaagc agttgtacat cacct
//
LOCUS       DAPHDW010000257         2577 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.263, whole genome shotgun sequence.
ACCESSION   DAPHDW010000257 DAPHDW010000000
VERSION     DAPHDW010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.263"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..2502
                     /locus_tag="RH057_004442"
                     /pseudo
     CDS             1..2502
                     /locus_tag="RH057_004442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          606 a          676 c          904 g          391 t
ORIGIN      
        1 catgcggacg ggagcattac ccgcagcgag tatgatgagg cggggaggct gaaggcacag
       61 acggatgcgg cggggcggcg gacggagtac agcctgcata tggcgtcggg tgcggtgacg
      121 gcggtgacgg ggccggacgg caggacggta cggtatggct ataacagcca gcggcaggtg
      181 acgtcggtga cgtacccgga cgggcttcgc agcagccggg agtatgatga gaagggaagg
      241 ctgacggcgg agacctcgcg cagcggtgag acaacgcggt acagctatga tgacccggcg
      301 agtgagctgc cgacagggat acaggacgcg acgggcagta caaaacagat ggcatggagc
      361 cgttacggtc aactgctggc cttcacggac tgctcggggt acacgacgcg gtatgagtac
      421 gaccggtacg gtcaacaaat cgccgttcac cgggaggaag gcatcagcac ttacagcagt
      481 tataacccgc gtgggcaact ggtcagtcag aaggatgcgc aggggcgtga aatccgttat
      541 gagtacagcg ccgcaggcga cctgacagca acgatatcac cggacggtaa acgcagcacc
      601 atcgaatatg ataaacgcgg gcgtccggtg agtgtgacgg aaggcggtct tacgcgcagc
      661 atggggtatg acgctgccgg gcgcatcacc gtgctgacca acgagaacgg cagccagtcc
      721 acgttccggt atgacccggt ggacaggctg actgaacagc gcggttttga cggccggacg
      781 caacgttacc actatgacct gaccggaaaa ctcacgcaga gtgaagacga ggggcttatc
      841 accctctggc actacgatgc gtcggaccgc atcacgcacc ggacggtgaa cggcgacccg
      901 gcagagcagt ggcagtatga tgagcacggg tggttaacca ccctcagcca tacctgtgaa
      961 ggccaccgcg tgtcggtcca ctacggctat gacgataaag gccgcctgac gggtgaacgg
     1021 cagacggtgg agaacccgga gacaggggag atgctgtggg agcatgagac ggggcatgcg
     1081 tacagcgaac aggggctggc gacccgtcag gagccggacg gtctgccgcc ggtggagtgg
     1141 ctgacgtatg gcagcggtta tcttgcgggg atgaagctgg gcggaacgcc gctggtcgag
     1201 tacacgcggg accggctgca ccgcgagacg gcccgtagct tcggcggggc gggcagtaca
     1261 gcggggtacg aacaggccac ggcgtacacc ctcacagggc agctacagag caggcacctg
     1321 aaccttccgc agcttgactg tgactatacg tggaacgata acggacaact ggtgcgcatc
     1381 agcggcccgc aggagtgccg ggagtaccgt tacagcggca cgggaaggct gacgggcgtc
     1441 cacactacgg cagcgaacct ggatatcgat atcccgtatg cgacggaccc ggcaggaaac
     1501 cggctgccgg acccggaact gcacccggac agcaccctca cggcgtggcc ggataaccgc
     1561 atcgcggaag atgcgcacta tgtctaccgc tacgatgaat acggcaggct ggcggagaag
     1621 acggaccgca tcccggaagg ggtgatacgg atgcacgacg agcggaccca ccactaccac
     1681 tacgacagcc agcaccgtct ggtgttctac acgcggatac agcacggtga gccgcaggtg
     1741 gagagccgct acctctatga cccgctgggc cgccggacgg gaaaacgggt gtggcggcgg
     1801 gagcgtgacc tgacggggtg gatgtcgctg tcgcgtaaac cggaggagac ctggtacggg
     1861 tgggacggtg acaggctgac cactgtacag acccaacaga caagaatcca gacggtatac
     1921 cagccgggaa gcttcacgcc gctcctgaga atcgaaacag agaatggtga acaggcgaag
     1981 gcgcggcacc gtagcctggc ggaggtgttg caggaggaca cgggtgtgac gctaccggcg
     2041 gagctggcgg tgatgctggg gaggctggag cgggaactgc ggcagggcag cgtgagtgaa
     2101 gaaagccagc agtggcttgc gcagtgcggg ctgacggcgg aacagatggc cgcgcaactg
     2161 gaggcggaat acatcccgga gaggaaactt catctttacc actgtgacca ccgtgggctg
     2221 ccgctggcgc ttatcagccc ggaaggggaa acggcgtggc agggggagta tgacgagtgg
     2281 ggaaacctgc tgggcgagga aagcgcgcag caccttcagc agtcactccg tctgccggga
     2341 cagcagtatg atgaggagtc ggggctgtac tacaaccgca accggtacta tgatccgttg
     2401 caggggagat atatcaccca ggacccgata gggctgagag gtgaatggaa tctgtataaa
     2461 tatccattaa accctgttcg gtttatagat tctttgggtt taaaattcca tgttaacggt
     2521 gatccaagtg attttaatca ggctgttgag tatttaaaac aagattcaca aatgaaa
//
LOCUS       DAPHDW010000258         2569 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.264, whole genome shotgun sequence.
ACCESSION   DAPHDW010000258 DAPHDW010000000
VERSION     DAPHDW010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2569)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2569)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2569
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.264"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            131..1297
                     /gene="ugd"
                     /locus_tag="RH057_004443"
     CDS             131..1297
                     /gene="ugd"
                     /locus_tag="RH057_004443"
                     /EC_number="1.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HDV3130688.1"
                     /translation="MKITISGTGYVGLSNGLLIAQNHEVVALDILPSRVAMLNDRISP
                     IVDKEIQQFLQSDKIHFNATLDKNEAYRGADYVIIATPTDYDPKTNYFNTSSVESVIK
                     DVVEINPYAVMVIKSTVSVGFTAAMHKKYRTENIIFSPEFLREGKALYDNLHPSRIVI
                     GERSERAERFAALLQEGAIKQNIPTLFTDSTEAEAIKLFANTYLAMRVAYFNELDSYA
                     ESLGLNTRQIIEGVCLDPRIGNHYNNPSFGYGGYCLPKDTKQLLANYQSVPNNLISAI
                     VDANRTRKDFIADAILSRKPQVVGIYRLIMKSGSDNFRASSIQGIMKRIKAKGVEVII
                     YEPVMKEDSFFNSRLERDLATFKQQADVIISNRMAEELKDVADKVYTRDLFGSD"
     gene            1463..1720
                     /locus_tag="RH057_004444"
                     /pseudo
     CDS             1463..1720
                     /locus_tag="RH057_004444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1788..2549
                     /locus_tag="RH057_004445"
                     /pseudo
     CDS             1788..2549
                     /locus_tag="RH057_004445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_047234906.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS30 family transposase"
BASE COUNT          787 a          557 c          567 g          658 t
ORIGIN      
        1 tcttataaag aaattctgaa ttgtttttcg ttgcgttgac catcgaacaa cgtagcgtta
       61 aaacttttat ctcttatcag gatgctaaaa acatcatgat tcacagttaa gttaattctg
      121 agagcatgaa atgaaaatca ccatttccgg tactggttat gtaggcttgt caaacgggct
      181 tctaatcgca caaaatcatg aggttgtggc attagatatt ttaccgtcac gcgttgctat
      241 gctgaatgat cggatatctc ctattgttga taaggaaatt cagcagtttt tgcaatcaga
      301 taaaatacac tttaatgcca cattagataa aaatgaagcc taccgggggg ctgattatgt
      361 catcatcgcc actccaaccg actatgatcc taaaactaat tatttcaata catccagtgt
      421 agaatcagta attaaagacg tagttgagat aaatccttat gcggttatgg tcatcaaatc
      481 aacggtttcc gttggtttta ccgcagcgat gcataagaaa tatcgtactg aaaatattat
      541 attctcaccc gaatttctcc gtgaaggtaa agccctttac gataaccttc atccgtcacg
      601 tattgtcatc ggtgagcgtt cagaacgcgc agaacgtttc gctgcgttat tacaggaagg
      661 ggcaattaag caaaatatcc caaccctgtt taccgactct actgaagcag aagcgattaa
      721 actatttgca aatacctacc tggcgatgcg cgtggcgtac tttaacgaat tggatagcta
      781 tgcagaaagt ttaggtctga atactcgcca gattatcgaa ggcgtttgtc tcgatccgcg
      841 tattggcaac cattacaaca acccgtcgtt tggctatggt ggttattgtc tgccgaaaga
      901 taccaagcag ttactggcga actaccagtc tgtgccgaat aatctcattt cggcaattgt
      961 cgatgctaac cgcactcgta aagactttat tgccgatgcc attttgtcac gcaaaccgca
     1021 agtggtgggt atttatcgtc tgattatgaa gagcggttca gataacttcc gtgcgtcttc
     1081 cattcagggg attatgaaac gtatcaaagc gaaaggcgtt gaagtgatca tttacgagcc
     1141 ggtgatgaaa gaagactcat tcttcaactc tcgcctggaa cgtgatctcg ccaccttcaa
     1201 acaacaagcc gacgtcatta tctccaaccg tatggcagaa gagcttaagg atgtggcaga
     1261 caaagtctac acacgagatc tctttggcag cgactaatct tctctgatca gggcggtttc
     1321 gccctgattg tcttttgtaa cctttgcaat aattcattgt ttttatcatt tatcctatat
     1381 gaagtggcac tgaagtggca cactgaattt ggccacctga acagaggtga tatgctcacc
     1441 tcagaacaac acaggtgctc caatgaaaaa aagaaatttc agcgcagagt ttaaacgcga
     1501 atccgctcaa ctggttgttg accagaacta cacggtggca gatgccgcca aagctatgga
     1561 tgttggcctt tccacaatga caagatgggt caaacaactg cgtgatgagc gtcagggcaa
     1621 aacaccaaaa gcctctccga taacaccaga acaaatcgaa atacgtgagc tgaggaaaaa
     1681 gctacaacgc attgaaatgc agaatgaaat attaaaaaag gctactgtag attcaattgg
     1741 tcaacgcaac agttatgtga aaacatgggg ttgcggaggt tttttgaatg agacgaacat
     1801 ttacagcaga ggaaaaagcc tctgtttttg aactatggaa gaacggaaca ggcttcagtg
     1861 aaatagcgaa tatcctgggt tcaaaacccg gaacgatctt cactatgtta agggatactg
     1921 gcggcataaa accccatgag cgtaagcggg ctgtagctca cctgacactg tctgagcgcg
     1981 aggagatacg agctggtttg tcagccaaaa tgagcattcg tgcgatagct actgcgctga
     2041 atcgcagtcc ttcgacgatc tcacgtgaag ttcagcgtaa tcggggcaga cgctattaca
     2101 aagctgttga tgctaataac cgagccaaca gaatggcgaa aaggccaaaa ccgtgcttac
     2161 tggatcaaaa tttaccattg cgaaagcttg ttctggaaaa gctggagatg aaatggtctc
     2221 cagagcaaat atcaggatgg ttaaggcgaa caaaaccacg tcaaaaaacg ctgcgaatat
     2281 cacctgagac aatttataaa acgctgtact ttcgtagccg tgaagcgcta caccacctga
     2341 atatacagca tctgcgacgg tcgcatagcc ttcgccatgg caggcgtcat acccgcaaag
     2401 gcgaaagagg tacgattaac atagtgaacg gaacaccaat tcacgaacgt tcccgaaata
     2461 tcgataacag acgctctctg gggcattggg agggcgattt agtctcaggt acaaaaaact
     2521 ctcatatagc cacacttgta gaccgaaaat aacgttatac gatcatcct
//
LOCUS       DAPHDW010000259         2568 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.265, whole genome shotgun sequence.
ACCESSION   DAPHDW010000259 DAPHDW010000000
VERSION     DAPHDW010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.265"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            82..1695
                     /gene="mppA"
                     /locus_tag="RH057_004446"
     CDS             82..1695
                     /gene="mppA"
                     /locus_tag="RH057_004446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HDV3130689.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKDRV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFTPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSTHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(1746..>2568)
                     /gene="ynaI"
                     /locus_tag="RH057_004447"
     CDS             complement(1746..>2568)
                     /gene="ynaI"
                     /locus_tag="RH057_004447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HDV3130690.1"
                     /translation="KFITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITS
                     ARIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIM
                     LYFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPG
                     RMTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNI
                     MVYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          735 a          582 c          625 g          626 t
ORIGIN      
        1 ttttgatgga agttgtttgc atttctttaa ggcgaaacaa ataattacgc atcaatttta
       61 atgtcggtta gagggaaact tatgaagcac tctgtttcag tcacgtgttg tgcgctgttg
      121 gtcagcagca tttctctttc gtatgctgca gaagttccga gcggcacagt actggcagag
      181 aagcaggagc tggtgcgcca cattaaagat gagcctgcgt cgctggatcc cgctaaagcc
      241 gtgggcctgc cagagattca ggtcattcgc gatctgtttg aaggactggt gaatcagaac
      301 gagaaagggg agattgtccc cggcgttgcg actcagtgga aaagtaatga caaccgtatc
      361 tggactttta ccctgcgcga taacgcaaaa tgggcggatg gcacaccggt aacggcgcaa
      421 gattttgtct acagctggca gcgtctggtg gacccgaaaa cgctatcgcc atttgcatgg
      481 tttgccgcgc tggcgggaat caacaacgca caggcgatta ttgatggtaa agctacgcct
      541 gaccagcttg gcgtcaccgc agttgatgcc cataccttga aaattcagct tgataaaccg
      601 ttgccgtggt ttgtgaattt aaccgctaac tttgccttct tcccggtgca aaaagccaac
      661 gtagaaagcg gtaaagagtg gacgaaaccc ggaaatctga tcggcaatgg cgcttatgtt
      721 cttaaagatc gcgtagtcaa tgaaaaactg gtcgtggtac cgaataccca ttattgggat
      781 aacgccaaaa cggtactgca aaaagtgacc ttcctgccaa ttaatcagga atccgcagcc
      841 actaagcgtt acctcgcggg ggatattgat atcaccgaat ccttcccgaa aaatatgtat
      901 cagaagctgt tgaaggatat tccggggcag gtttatacgc cgccacagct cgggacctat
      961 tattatgcgt ttaacacgca aaaagggccg acggcagatc agcgcgttcg cctggcatta
     1021 agtatgacga tagatcgccg cctgatgacc gaaaaagtgt tgggaacggg cgaaaagcca
     1081 gcgtggcatt ttacaccaga tgttaccgcc ggatttacgc cggaaccttc gccgtttgaa
     1141 caaatgagtc aggaagaact gaatgcacag gcaaaaactt tgttgagcgc agctggttat
     1201 ggtccgcaaa aaccgctgaa gctgacgctt ttgtataaca cttcagaaaa ccatcaaaaa
     1261 attgcgattg ctgtagcatc gatgtggaaa aagaaccttg gcgtagatgt taaattgcaa
     1321 aatcaggaat ggaaaaccta tatcgatagc cgtaacaccg gcaattttga tgttatccgt
     1381 gcctcatggg tgggggatta taatgaaccc tccactttcc tgacattatt aacgtcaacg
     1441 cattcaggaa atatttcacg ctttaacaat ccggcatacg acaaagttct ggcccaggca
     1501 tcgacggaaa ataccgttaa agcgcgtaat gccgattaca acgcggcaga aaaaatcctc
     1561 atggagcaag caccgattgc accaatttat caatatacca atggacgatt aatcaagccg
     1621 tggctgaaag gttatcccat taataacccg gaagatgtgg cgtacagccg gactatgtat
     1681 attgtgaagc attgatgtga tgggaactgg cgttaccctt gtggataacg ccagtgatgt
     1741 ctgatttagc gaccctgttc cggcggtgta atgttatcca tatacagcgt ctggctgggg
     1801 aaggcaaagt ccgcgccgtg tgactgtaca atatcgataa tcttcaaata aacgtcttgc
     1861 tgtgcagcaa gccactcagc ccataccgtg gttttggtaa agcaataaac cataatattc
     1921 aatgacgagt cggcaaactg gttgaaataa accagtaagg tttgtcgctg gtcgatggcc
     1981 gggtgatttt tcagcatctc acgtacggct tcgacaataa cgcccacttt tgccgcatcc
     2041 tcataacgta aaccaatggt cgtggtaatg cggcggttgg tcattcgtcc tgggttttct
     2101 acgctgatcg atgaaaacag cgagttcggt acgtacaatg gacgattatc aaaggtcgta
     2161 attttggtaa ttcgccagcc aatttccgct actgtacctt cgatatttct gtccggtgaa
     2221 cggatccagt cgccaatact gaaaggacgg tcgaaataga gcatgatccc ggaaaagaaa
     2281 ttactcagaa tatctttacc ggccataccg acagccagac caccaatacc accaaaggtc
     2341 agcaagccag aaaggctcat gccgaaatgt tcgccataaa gcagaacaag caccacaata
     2401 atggtgattt tgataatgcg cgacataatc cgcgcactgg tgatatcgcg acctttttta
     2461 atctgctgtt tttcaaactg attaatcagc aaaaatagct taatcgtcag aataaccgca
     2521 atcagggacg tacagataaa atcgataacg cctggggtga taaatttg
//
LOCUS       DAPHDW010000260         2514 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.266, whole genome shotgun sequence.
ACCESSION   DAPHDW010000260 DAPHDW010000000
VERSION     DAPHDW010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.266"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            343..486
                     /locus_tag="RH057_004448"
     CDS             343..486
                     /locus_tag="RH057_004448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HDV3130691.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            499..708
                     /gene="cspE"
                     /locus_tag="RH057_004449"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             499..708
                     /gene="cspE"
                     /locus_tag="RH057_004449"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HDV3130692.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            874..1683
                     /gene="rlmA"
                     /locus_tag="RH057_004450"
     CDS             874..1683
                     /gene="rlmA"
                     /locus_tag="RH057_004450"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HDV3130693.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMIQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADVLPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSDELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1680..2246)
                     /gene="mntP"
                     /locus_tag="RH057_004451"
     CDS             complement(1680..2246)
                     /gene="mntP"
                     /locus_tag="RH057_004451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HDV3130694.1"
                     /translation="MNITATVLLAFGMSMDAFAASIGKGATLHKPKFSEALRTGLIFG
                     AVETLTPLIGWGMGMLASRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRR
                     HGFWLLVTTAIATSLDAMAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGS
                     IIGKKAEILGGLVLIGIGVQILWTHFHG"
BASE COUNT          671 a          597 c          609 g          637 t
ORIGIN      
        1 cgttaactga ctgttttatg agaaaaagtg atataacttt ttattcattg catagcaaaa
       61 aatgtgatat tgcacgcact atgtaataac ttctcccact ggcctggaac aactgaactt
      121 attgaactat gttagaaaat acgccagttt aagtatctgc ctgaactggc aaggttaagc
      181 acaatgatat atcggcgcgt attccgttgc ataagtgtgc aaaaaaagtg gaagacgtat
      241 cgagatttgt gcgtctgatc gagacatgtt taaaaatggc ttgccataat taacgttgta
      301 tgtgataaca gatttcgggt taaacgaggt acagttctgt ttatgtgtgg cattttcagt
      361 aaagaagtcc tgagtaaaca cgttgacgtt gaataccgct tctctgccga gccttatatt
      421 ggtgcctcat gcagtaatgt gtcagtttta tctatgttat gcctgcgggc gaagaaaaca
      481 atctaaggaa tttttcaaat ggcaaagatt aaaggtcagg ttaagtggtt caacgagtct
      541 aaaggttttg gcttcattac tccggctgat ggcagcaaag atgtgttcgt acacttctcc
      601 gctatccagg gtaatggctt caaaactctg gctgaaggtc agaacgttga gttcgaaatt
      661 caggacggcc agaaaggtcc ggcagctgtt aacgtaacag ctatctgatc gaatccactg
      721 atctgaagtg tgaatacgct tcaatctcgc tataaagcct cgtcgaatgc gaggcttttt
      781 actatgcttt atcttcgctc ctggcgttcg gatatttgcc cgccgcgtga ttcgcgttac
      841 acttgcggcc tttagtatcc tgccggagtt gtcatgtcgt tttcctgtcc actttgccat
      901 cagcctcttt cgcgtgaaaa aaacagctat atctgtcccc agcgacatca gtttgatatg
      961 gcgaaagaag ggtatgtcaa tctgctgccc gttcagcata aacggtctcg tgatccgggc
     1021 gacagcgcgg aaatgataca agcacgccgc gcattcttag atgccggaca ttatcagccg
     1081 ctgcgtgatg caattgtcgc ccaactgcgg gaacggcttg atgaaaaggc cacggcggtg
     1141 ctggatattg gctgtggtga agggtattac acacacgcat ttgccgatgt gttgcccgaa
     1201 atcaccacgt ttggcctgga tgtctcgaag gtagcgataa aagcggcggc gaaacgctat
     1261 ccgcaggtca ctctttgtgt cgcttccagc caccgtttgc cgttttccga taccagtatg
     1321 gacgccataa tacgtattta cgcgccgtgt aaagcagaag aattagcacg agtagtgaag
     1381 cccggcggct gggtcattac tgccacgccg ggaccgcgac atttgatgga gctgaagggg
     1441 ctgatttaca atgaagtaca tcttcatgca cctcatgcag aacaactgga aggttttaca
     1501 ttacagcaga gtgatgagtt gtgttatccg atgcgtcttc gcggtgatga agccgtcgca
     1561 ttattgcaga tgacgccgtt tgcctggcgt gcgaagccag aagtctggca aacactggca
     1621 gcaaaagaag tgttcgactg ccagacggac tttaatattc acctctggca gcgttcttat
     1681 taaccgtgga agtgcgtcca gaggatctgg acgccgatgc cgatcagcac cagcccgccg
     1741 agaatttccg cttttttccc aataattgag ccgataaagc gaccaaccat catccctaat
     1801 gtcgacataa tcaaggttgc acaaccaatg gccaatgcgg tcgcgataat gttgacctgc
     1861 aggaaagcaa gaccaacacc cacagccatg gcatccaggc tggtggcaat cgcggtggtt
     1921 accagtagcc agaaaccgtg ccgacggcgc ggctcttcat cttcatcatc tgcgccacga
     1981 aaaccctcaa taatcattcg cccgccgagg aatatcagca gcacaaacgc aatccagtgg
     2041 ttccattcaa ggacaaaccg gctggctaac atgcccattc cccagccgat cagcggcgtc
     2101 agggtttcga cggcaccaaa aataaggccg gttcgcaatg cttcagaaaa tttcggttta
     2161 tggagggtgg cacctttacc gattgatgca gcaaatgcat ccatcgacat accaaacgca
     2221 agaagaacag tagcagtgat attcatgaca atgtcctgac cggggtatcc ataaaacaca
     2281 tcatcgcccc cagcaaacat gcatacatga acaacaagcc gtgatggttt tcgcgttcat
     2341 gcaggcatag cgattgatgt gcatatggtc tcgcctgact gaaagtattc agtccgtacg
     2401 tgccacgttt tgcaacgagt atgttgacac gtacatttcc ggaatctgga aatcggctac
     2461 tccccaaatg agggcgcaac cttaacatat tttgagaata taaaacaact gttg
//
LOCUS       DAPHDW010000261         2494 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.267, whole genome shotgun sequence.
ACCESSION   DAPHDW010000261 DAPHDW010000000
VERSION     DAPHDW010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.267"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            55..1179
                     /locus_tag="RH057_004452"
     CDS             55..1179
                     /locus_tag="RH057_004452"
                     /inference="COORDINATES: protein
                     motif:HMM:NF017411.1,HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3130695.1"
                     /translation="MASGAVTAVTGPDGRTVRYGYNSQRQVTSVTYPDGLRSSREYDE
                     RGRLTAETSRSGETTRYSYDDPASELPTGIQDATGSTKQMAWSRYGQLLAFTDCSGYT
                     TRYEYDRYGQQTAVHREEGISTYSSYNPRGQLVSQRDAQGRETRYEYSAAGDLTATVS
                     PDGKRSTIEYDKRGRPVSVTEGGLTRSMGYDAAGRITVLTNENGSQSTFRYDPVDRLT
                     EQRGFDGRTQRYQYDLTGKLTQSEDEGLITLWHYDASDRITRRTVNGEPAEQWQYDDH
                     GWLTEISHLSEGHRVAVHYGYDDKGRLTGERQTDGEDGPHPDGCYPDGRRADPPLPLR
                     QPAPPGVPHADTAWRATGREPLPLRPAGTANGETGMAAGA"
     gene            1148..2338
                     /locus_tag="RH057_004453"
     CDS             1148..2338
                     /locus_tag="RH057_004453"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3130696.1"
                     /translation="MAKRVWRRERDLTGWMSLSRKPEETWYGWDGDRLTTVQTDTTRI
                     QTVYQPGSFAPLIRIETDNGEREKAQCRSLAEKIQQEGSEDGHGVVFPAELVGLLDRL
                     EGEIRANCVSSESRQWLAQCGLTVERLAAQIEPVYLPERKIHLYHCDHRGLPLALISE
                     DGNTAWSAEYDEWGNQLNEENPHHLHQPYRLPGQQYDKESGLYYNRHRYYDPLQGRYI
                     TPDPIGLRGGWNMYQYPLNPIQVIDPMGLDAIENMTSGGLIYAVSGVPGLIAANSITN
                     SAYQFGYDMDAIVGGAHNGAADAMRHCYLMCRMTKTFGSTIADVIGKNHEAAGDRQGQ
                     PAKERIMDLKNNTVGIACGDFSAKCSDACIEKYNTGQLFGLDGIKADNPIKTKQGSSD
                     ASNY"
BASE COUNT          639 a          591 c          797 g          467 t
ORIGIN      
        1 aggctgaagg cgcagacgga tgcggcgggg cggcggacgg agtaccggct gcatatggcg
       61 tcgggcgcgg tgacagcggt gacggggccg gacggcagga cggtgcggta tggctataac
      121 agccagcggc aggtgacgtc agtgacgtac ccggacgggc ttcgcagcag ccgggagtat
      181 gatgagaggg gaaggctgac ggcggagacc tcgcgcagcg gtgagacaac gcggtacagc
      241 tatgatgacc cggcgagtga gctgccgaca gggatacagg acgcgacggg cagtacaaaa
      301 cagatggcat ggagccgtta cggtcaactg ctggccttca cggactgctc ggggtacacg
      361 acgcggtatg agtacgaccg gtacggtcaa caaaccgccg ttcaccggga agaaggcatc
      421 agcacttaca gcagttataa cccgcgcggt caactggtca gtcagaggga tgcgcagggg
      481 cgggagaccc gttatgagta cagcgccgca ggcgacctga cagcaacggt atcaccggac
      541 ggtaaacgca gcaccatcga atatgataaa cgcgggcgtc cggtgagtgt gacggaaggc
      601 ggtctgacgc gcagcatggg gtatgacgct gccgggcgca tcactgtgct gaccaacgag
      661 aacggcagcc agtccacgtt ccggtatgac ccggtcgaca ggctgacgga gcagcgcggc
      721 tttgacggcc ggacgcaacg ttaccagtat gacctgaccg gaaaactcac gcagagtgaa
      781 gatgaggggc ttatcaccct ctggcattac gatgcgtcgg accgtatcac ccggcgcacg
      841 gtgaacggtg aaccggcgga gcagtggcag tatgacgacc acggctggct gacagaaatc
      901 agccacctga gtgaaggcca ccgtgtggcg gtccactacg gctatgacga taaaggccgt
      961 ctgacgggcg aacggcagac tgacggagaa gacggaccgc atcccgacgg gtgttatccg
     1021 gacggacgac gagcggaccc accactacca ctacgacagc cagcaccgcc tggtgttcca
     1081 cacgcggata cagcatggcg agccactggt cgagagccgc tacctctacg acccgctggg
     1141 acggcgaatg gcgaaacggg tatggcggcg ggagcgtgac ctgacggggt ggatgtcgct
     1201 gtcgcgtaaa ccggaggaga catggtacgg ctgggacggc gacaggctga cgacggtaca
     1261 gaccgacacc acgcgtatcc agacggtata ccagccgggg agcttcgcgc cgctcatccg
     1321 catcgaaaca gacaacggtg aacgggagaa agcgcagtgc cgcagcctgg cggagaagat
     1381 acagcaggaa gggagcgagg acgggcacgg cgtggtattt ccggctgaac tggtggggct
     1441 gctggacagg ctggaggggg aaatccgggc aaactgcgtg agcagtgaaa gccgccagtg
     1501 gcttgcgcag tgcgggctga cggtggagcg gctggcggca cagatagagc cggtttattt
     1561 gcctgagcga aaaatacatc tttatcactg tgaccaccgg ggcctgccgc tggcgcttat
     1621 cagtgaagac ggcaatacgg cgtggagcgc ggaatatgat gaatggggca accagcttaa
     1681 tgaggagaac ccgcatcacc tgcaccagcc gtaccggctg ccggggcagc agtatgataa
     1741 ggagtcgggg ctgtactata accgtcaccg gtactacgat ccgttgcagg ggcggtatat
     1801 caccccggac ccgattgggt tgagaggtgg atggaatatg tatcagtatc cgttgaatcc
     1861 catacaagtg atagacccaa tggggttaga tgcgattgag aatatgacat caggtggact
     1921 aatttatgcc gtatctggtg tacctggatt gattgctgca aacagcatta ctaacagtgc
     1981 ttaccagttc ggttatgata tggatgctat tgttggcgga gctcataatg gggccgccga
     2041 tgcaatgaga cattgttact tgatgtgtcg aatgactaag acatttggat caacaatagc
     2101 tgacgtgata ggtaaaaatc atgaggcggc aggggataga caaggtcagc cagctaaaga
     2161 aagaatcatg gatcttaaaa ataacactgt cggtattgct tgtggcgatt tttctgccaa
     2221 atgtagcgat gcatgtattg aaaaatataa cactgggcaa ctcttcgggt tagatggtat
     2281 aaaagcagat aatccaataa aaacaaagca agggagttca gatgcttcaa attattagag
     2341 gcaaacttgt cattttttta attacccttt gtttatttgt tgtttacctt gggtttgata
     2401 acaattcaaa ttctgacatc gtattttatg gacataaaac accaaagagc gttgagatat
     2461 atctttctga aaaaaatatt atttataaaa taat
//
LOCUS       DAPHDW010000262         2482 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.268, whole genome shotgun sequence.
ACCESSION   DAPHDW010000262 DAPHDW010000000
VERSION     DAPHDW010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2482)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2482)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2482
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.268"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(103..440)
                     /locus_tag="RH057_004454"
                     /pseudo
     CDS             complement(103..440)
                     /locus_tag="RH057_004454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001604131.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="filamentous hemagglutinin"
     gene            complement(418..777)
                     /locus_tag="RH057_004455"
     CDS             complement(418..777)
                     /locus_tag="RH057_004455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF596 domain-containing protein"
                     /protein_id="HDV3130697.1"
                     /translation="MTLLLNDKQYNELCEAAEGRNLGAVFSYSEPEEPPPLNFSFEER
                     KKVFLWVLTRLLKEGRIKLAKHGKFLEGSVEEQVERFRQAFPKTEEEMEDGIWFFDES
                     CPGGAVWGLEDGSLEWT"
     gene            926..2461
                     /locus_tag="RH057_004456"
                     /pseudo
     CDS             926..2461
                     /locus_tag="RH057_004456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          701 a          577 c          555 g          649 t
ORIGIN      
        1 tgggtgtgta ttatcgtgaa ggagatcggt ggggataacc tccattgaaa ttggagttac
       61 tcgccgatca ctttaacaat aatacgcatc tgtggtatca ttccccacca cctcgccgga
      121 ggcaacacct gcagcaccgg taagggtatt atttccctgt gccaccgcca gtacagcatt
      181 caccacggca tgggctacca cacgccccgc accgtccggg ggtctgtttg ataatttcag
      241 caatatacgg cgcagcacca tctgccaagg ctgcactcag attaccgcca gccagtccct
      301 gtaccgctgc cgttgcagcc tgtatcgcct gctggtattg ccccccatcc caaaaccgga
      361 gccgccatat tgttgttata cgctcgctgc gccgcatcct gcgctgtgaa gggtttatca
      421 ggtccattcg agagagccat cttcaagccc ccaaacagct ccccccggac aactctcatc
      481 aaaaaaccag atgccgtctt ccatctcttc ttctgttttc ggaaatgcct gtcggaaacg
      541 ctcaacctgc tcttcaacac ttccttccag aaacttaccg tgcttggcga gtttgatacg
      601 cccttctttc agaagacggg tcaataccca taaaaaaact tttttccttt cttcaaatga
      661 aaaatttaac ggcgggggtt cttctggttc actataggaa aaaacagccc ccaaattacg
      721 accttctgca gcttcacata attcattata ttgcttgtca ttaagtaata atgtcattta
      781 aattttatct ccacatcttt gtatttatat gcaatgtcag aaagttgttt attcccctta
      841 atgcgtattt tcatgaaagg agatcactca acaacccctt gtgtcagcat agttgtcgcc
      901 tagattttta cacaggagag agactatgcc cgcacatgca gtaccaattg atataaaaga
      961 acagggcgtt acctggttat taccacctta tagttggtcc atacggcata ttgctcgtaa
     1021 actggacctg agcccttcag taatagcaag atggagactt gaactggtgc aacaaggcct
     1081 gttacctgaa aatgaaaaaa tatcttctgt cgatggaaat gaatggacac cggagcgacg
     1141 attttctgtt gtaattgaaa ctgcaacatt atctgaaatg gaactggctg attattgtcg
     1201 gagaatgggg ttatttgtcg agcaagttaa agaatggaga gctatatcca ttcaggctca
     1261 tgaaatgaaa atggcagaaa gtcgccgtgc tgataaagaa ttacgcaaag caaagacccg
     1321 gttacgggaa ctggaaaaag aacttctccg taaagataaa gctctggcgg aagcaactgc
     1381 tcttttgatg ttaagggaaa agttcaatgc cctctgggac aacagcgagg aaggctgact
     1441 cctcttccag agcggcagaa gattattaat ctggtcagcg aggctgtcag ttcaggagcg
     1501 agaaaaacac aagcctgcca gtgtattggt atttcagtca gaacacttcc acgttggatg
     1561 cagggtggtg tggttcatgc agatcagaga ccaggtgcat caaggcgtac cccggggaat
     1621 aaactgtcag aaacagaaag attaagaata cttgatgtat gcaattctcc ggagtttgca
     1681 tcattgtctc cgaatattat tgttccgact cttgccgaca gaggggttta tatcgcttca
     1741 gaatcaactt tttatcgcgt attaaaatca gctaaccaac tgacaaggag aacccgtgaa
     1801 caccaatata agcggcctga agtactcaaa gctgtagccc ccaaccaggt gtggatctgg
     1861 gatattagtt atatcccatc atgggttaag gggcagcatt tttatctcta tatgatcgtc
     1921 gatatttata gccgaaaaat tatagctgcc gaagtattta cgaaagaatc tgggcagtat
     1981 gcggcagaac tgcttcaacg tgctgtatgg aatgaaaaat gttcatcccg caatctcgtg
     2041 cttcattctg ataacggagg accaatgcgc agttgtacgt tactggcaaa aatgtacgct
     2101 cttggcgtat taagttctta ttcccgtccc ctggtcagca atgataaccc ttattccgaa
     2161 tcgctcttcc ggacgattaa gtattgtcca tggtggccag agaatggttt tcgaaccata
     2221 gatgaagcca gaagttggtt aagcaggttc acgcgatggt acaacctgga acacaagcca
     2281 gcggtattaa gtatgttact ccggatgaac gccatcgcgg aattgatgca caaatactgg
     2341 aagccagaaa aaagtttatc gcgaagctcg caaacgtcat cctgagcgct ggagcaaaca
     2401 gttgcgtgac tgggaactca ttcaggcagt ttatctgaat ccagaaaaag aagcggcata
     2461 actgtaattt tattacatca gg
//
LOCUS       DAPHDW010000263         2481 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.269, whole genome shotgun sequence.
ACCESSION   DAPHDW010000263 DAPHDW010000000
VERSION     DAPHDW010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2481
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.269"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            98..970
                     /locus_tag="RH057_004457"
     CDS             98..970
                     /locus_tag="RH057_004457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HDV3130698.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTRHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVRRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILHRGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQAQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            1298..>2481
                     /gene="ag43"
                     /locus_tag="RH057_004458"
     CDS             1298..>2481
                     /gene="ag43"
                     /locus_tag="RH057_004458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV3130699.1"
                     /translation="MKRHLNTSYRLVWNHITGTLVVASELARSRGKRAGVAVALSLAA
                     VMSVPALAADIVVRPGETVNGGTLANHDNQIVFGTTNGMTISTGLEYGPDNEANTGGQ
                     WVQDGGTANKTTVTSGGLQRVNPGGSVSDTVISAGGGQSLQGRAVNTTLNGGEQWMHE
                     GAIATGTVINDKGWQVVKPGTVATDTVVNTGAEGGPDAENGDTGQFVRGDAVRTTINK
                     NGRQIVRTEGTANTTVVYAGGDQTVHGHALDTTLNGGYQYVHNGGTASDTVVNSDGWQ
                     IVKKGGVAGNTTVNQKGRLQVDAGGTATNVTLKQGGALVTSTAATVTGINRLGAFSVV
                     EGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADSG
                     AAV"
BASE COUNT          570 a          661 c          721 g          529 t
ORIGIN      
        1 tcgattcaaa acaatttcag acagatatta tctccgtgaa tcccccgcca cctttccggt
       61 gcgcggggtt ttgtcttttt tcaccgggaa tacatgtatg aatccgtctg atgccattga
      121 ggcaattgaa aaaccgctct cctccctgcc ttactcgctt tcccgtcaca tcctggaaca
      181 tctgcgcaaa ctcacccgtc acgaacccgt gattggcatt atgggtaaaa gcggggccgg
      241 taaatcctca ctctgtaatg cactgtttca gggggaggtc accccggtca gtgatgttca
      301 cgccggcacc cgggaagtgc ggcgcttccg tctgagtggc cacggtcaca gcatggttat
      361 cactgacctg cccggggtgg gcgagagccg ggacagggat gcagagtatg aagccctgta
      421 ccgtgacatt ctgcccgaac tggacctggt gctgtggctg attaaagccg atgaccgcgc
      481 cctgtctgtg gatgagtatt tctggcgaca catcctgcac cggggacatc agcaggtgct
      541 gtttgtggtg acgcaggccg acaaaacgga gccctgccat gaatgggata tggccggtat
      601 tcagccttct cccgcacagg cacagaatat tcgcgaaaaa acggaggcgg tattccgtct
      661 gttccggccc gtacatccgg ttgtggccgt atcggcccgc accggctggg aactggatac
      721 gctggtcagt gcgctcatga cagcgcttcc cgaccatgcc gccagtcccc tgatgacccg
      781 actgcaggac gagctgcgca cggaatctgt ccgctctcag gcccgtgaac agtttaccgg
      841 tgcggtggac cggatatttg acacagcgga gagcgtctgt gttgcctctg ttgtccgtac
      901 ggccctgcgc gctgttcgtg acacggtggt ctctgttgcc cgtgcggtat ggaactggat
      961 tttcttctga acctgtcgtg actgatgccc tccctgactc tgagtctgct cacaaaagcg
     1021 ctgttttcgt tactgtctct cttgtccgtg caatagctca ataatagaat aaaacgatcg
     1081 atatctattt tatcgatcgt ttatatcgat cgataggcta ataataacct ttgtcagtaa
     1141 catgcacaga tacgtacaga aagacattca gggaacaaca gaaccacaat tcagaaactc
     1201 ccacagccgg acctccggca ctgtaaccct ttacctgccg gtatccacgt ttgtgggtac
     1261 cggctttttt attcaccctc aatctaagga aaagctgatg aaacgacatc tgaacaccag
     1321 ctacaggctg gtatggaatc acattacggg caccctggtg gtggcctccg aactggcccg
     1381 ctcacgggga aaacgcgccg gtgtggcggt tgctctgtct cttgctgctg tcatgtcagt
     1441 cccggcactg gctgctgaca tcgttgtacg cccgggagaa accgtgaacg gcggaacact
     1501 ggcaaatcat gacaaccaga ttgtcttcgg tacgaccaac ggaatgacca tcagtaccgg
     1561 gctggagtat gggccagata acgaggccaa taccggcggg caatgggtac aggatggcgg
     1621 aacagccaac aaaacgactg tcaccagtgg tggtcttcag agagtgaacc ccggtggaag
     1681 tgtctcagac acggttatca gtgccggagg cggacagagc cttcagggac gggcagtgaa
     1741 caccacgctg aatggtggcg aacagtggat gcatgagggg gcgatagcca caggaaccgt
     1801 cattaatgat aagggctggc aggtcgtcaa gcccggtaca gtggcaacgg ataccgttgt
     1861 taataccggg gcggaagggg gaccggatgc agaaaacggt gataccgggc agtttgttcg
     1921 cggggatgcc gtacgcacaa ccatcaataa aaacggtcgc cagattgtga gaactgaagg
     1981 aacggcaaat accactgtgg tttatgccgg cggcgaccag actgtacatg gtcacgcact
     2041 ggataccacg ctgaatgggg gataccagta tgtgcacaac ggcggtacag cgtctgacac
     2101 tgttgtgaac agtgacggct ggcagattgt caaaaaaggg ggtgtggccg ggaataccac
     2161 cgttaatcag aagggcagac tgcaggtgga cgccggtggt acagccacga atgtcaccct
     2221 gaagcagggc ggcgcactgg ttaccagtac ggctgcaacc gttaccggca taaaccgcct
     2281 gggagcattc tctgttgtgg agggtaaagc tgataatgtc gtactggaaa atggcggacg
     2341 cctggatgtg ctgaccggac acacagccac taatacccgc gtggatgatg gcggaacgct
     2401 ggatgtccgc aacggtggca ccgccaccac cgtatccatg ggaaatggcg gtgtactgct
     2461 ggccgattcc ggtgccgctg t
//
LOCUS       DAPHDW010000264         2400 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.270, whole genome shotgun sequence.
ACCESSION   DAPHDW010000264 DAPHDW010000000
VERSION     DAPHDW010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.270"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(59..497)
                     /locus_tag="RH057_004459"
                     /pseudo
     CDS             complement(59..497)
                     /locus_tag="RH057_004459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            501..671
                     /locus_tag="RH057_004460"
                     /pseudo
     CDS             501..671
                     /locus_tag="RH057_004460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014227705.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase"
     gene            complement(748..1576)
                     /locus_tag="RH057_004461"
                     /pseudo
     CDS             complement(748..1576)
                     /locus_tag="RH057_004461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465694.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            complement(1782..2012)
                     /locus_tag="RH057_004462"
     CDS             complement(1782..2012)
                     /locus_tag="RH057_004462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV3130700.1"
                     /translation="MQRHGSIETRTARDKAAERQMSHMDVTQRRPRIIHKAEYLDERK
                     LMLQWWVDFLDANRERAITPFDYAKINRGNGE"
     gene            2244..>2400
                     /locus_tag="RH057_004463"
     CDS             2244..>2400
                     /locus_tag="RH057_004463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130701.1"
                     /translation="MSAQEKTVQIDSFDAKFLDINTLSKLIYERKSFVIENVSDVSET
                     VKRVEHEI"
BASE COUNT          625 a          598 c          513 g          664 t
ORIGIN      
        1 ttaccttatg gagagattta tattttaaac gatatatttg tattacataa tggattttct
       61 accatatttc cagacatctg ttatcactta acccattact gttccaccgc cgcagacact
      121 cctgtggcga gtggtaacac caaagcacta tgcagatacc attagttata gtgtccgaat
      181 gcctccgcaa gttttttgct gccgttaacc cgtccggttt gggcatgata ctgatgtaat
      241 catttcacga agctctctgc tattccgtta ctctccggac tccgcaccgc cgtgttcttc
      301 ggttcaagtc ccaacatccg ggcgaactgg cgtgtttcat tagcccggta gcatgaacca
      361 ttatccgtta gccactccac tggatacgcc ggaagctcgc tgccaaagcg gcgttccacg
      421 gctcttggca tgacgtcctg tattgtttca ctgtcgaagc agcccgtagt gaccgcccag
      481 tgtagtgcct cacgctcgct gaagcgtttt ttcttggcgg tgtagagaca acaaaagtgc
      541 tgaaggcgtt taggccacac attttctttg atgataagga tgttgatttg atcgaggctg
      601 caaaggtagt accttctggt aaagtacctt atgccagtaa ctccgaactg gcagaacgtg
      661 aagcatcgtg attatcacta acaaaccatt tagtcattaa ggctggatgg tttgtatacc
      721 acacgcaaaa aactgcccta ccccagatca atgttctcct ctgaacacct cttttctctg
      781 ccattccaca aactgttcgc ccggataatt ttctttaacc ggtggcaacg caatcgcttt
      841 tcccgcaaaa tcccagaaca gtcgctatac cactccacca ccatttaccg caccggaaac
      901 caccacacgc atattttcat ccaggccaat cgaaccgcga tcaaacactt tatgatggat
      961 tgcgcacaat gccaggccat taggtatttc acaagggcca tggtgctgct tccatctgat
     1021 atgagcaact tccagagcaa tcggcgcatt gtcgtggcgc atgttaaagc cgcagatagc
     1081 gcactgataa ttgtaagcac gtagaaccat cttgcgaaac cccggatcgc gttgacggag
     1141 agaagtgcga atatcaaaat ccatctcgtc agcgatctct tcctgaatac tggccgggaa
     1201 attttgtcac caacgcaaaa ttatcggcat caaatccacc agcgacatta tattcaatga
     1261 gttcacgtcg gggcggctgg cggctaccac tggttgagca cagttccgcg ttatgtggtt
     1321 cccagaagcc atcgcccttc agacgccaga acggcatgtc cgggcgctgg tcacggcgct
     1381 gcggtccata acgttccagg agatccaaca ggggctcgta gatttcagaa gcatagttaa
     1441 acaggcgacc atgaccctgc cgatagtgtg agagaacgta cagcagcaat aatggcttat
     1501 gtggcgcacg ttgttcgcct ttgcgccaga tggtgatatt tgatatcgct tgctttaggg
     1561 ttttgtcaga ggtcattgcg ctggagaatc catgagcaca tgtttgcgat gatgctcgca
     1621 actatatgta cattcaaggc attattgcag acatcctttt tgcctgacgg ctctgtacct
     1681 ggcagatgga attctgaaaa aaatacagcg accaaccagt cctaagcggt ctggttgatc
     1741 ttttaaattt aaacatcagc taaggagtaa cggcattcaa ctcactcgcc attaccccga
     1801 ttgatcttcg catagtcaaa cggcgtaatc gctctttccc gattggcatc cagaaaatcg
     1861 acccaccact gcaacatcag cttacgctca tccagatatt ccgctttatg gattatacgc
     1921 ggcctgcgct gagttacgtc catgtggctc atctgacgtt cagccgcttt atctcgtgct
     1981 gttctggtct caatacttcc atgccgttgc aattacctct taaaccattc cttaccattt
     2041 tcatgtcttc gataagaaaa tttcatcatg catgatcgat tttgtcgatc gattcgatcg
     2101 aattttctct ttttaccctg ccttttgcat ggttttaaaa taggcaaaat tacttgtcat
     2161 tcgtggaaaa tgatggcgga gataactgag cttaaaaagt ttctcttgtc tccgtcactt
     2221 gccataccag ccaggaaaag aagatgtcag cacaagaaaa aacagtacag attgattctt
     2281 ttgatgcgaa atttttagat ataaatacat taagcaagct tatctatgaa cgtaagagtt
     2341 ttgtgataga aaatgtttcc gatgttagtg aaacggtaaa aagagtggag catgaaatag
//
LOCUS       DAPHDW010000265         2380 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.271, whole genome shotgun sequence.
ACCESSION   DAPHDW010000265 DAPHDW010000000
VERSION     DAPHDW010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.271"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..867)
                     /locus_tag="RH057_004464"
     CDS             complement(<1..867)
                     /locus_tag="RH057_004464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HDV3130702.1"
                     /translation="MQRKLATWAVTDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTK"
     gene            complement(1540..1740)
                     /locus_tag="RH057_004465"
                     /pseudo
     CDS             complement(1540..1740)
                     /locus_tag="RH057_004465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1740..2339
                     /locus_tag="RH057_004466"
                     /pseudo
     CDS             1740..2339
                     /locus_tag="RH057_004466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099160.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          512 a          701 c          631 g          536 t
ORIGIN      
        1 tttggtgcct ttgacgatga ggacaaaatc atcggcatag cggcagtacg ccaccgcggg
       61 tttccactgc cagttttctc tgaccgccgt acttcggccc cgttggatac tgttattcca
      121 gtaccaccga tcttttctgg ctttcccgct caggtagcgc tcatgcaggt attgatcgaa
      181 ctcattcagc atgatgttcg ataatagcgg cgatataaca ccgccctgtg gcacaccttc
      241 actggccgcc cgaaagagac cgacatcgat atgtcccgcc ttgatggttt tccacagcag
      301 agtcatgaaa cgtgcgtcac tgatcctgcg gcgtacagcc ttcatcagca gtcgatgatg
      361 tacggtgtcg aagtaactgg acaggtcgcc ttcaatcacc cagcgtcccc gggtttcacc
      421 acagtctgtg agctgtaatt tcaccgtgcg gatagcgtgg tggacactgc gctcaggccg
      481 gaagccatat gagagcgtat gaaaatcact ctcccatatc ggctccatcg ccatcagcat
      541 ggcccgctga acaatacgat cccgcaacgc ggggataccc agtggtcgca gtttgccgtt
      601 gcttttaggg atgtaaaccc gtctggcggg caagggctgg tagtggcctg agagtaattc
      661 atccctgagg atttgcagct caacagccag tctggcctgt agcattgttt tgttcacgcc
      721 atcaacgccg ggggtatggg ccccctttga tgaaagcgtg atccgcgccg cttcagccag
      781 ccattctggt tgtgttatca gacgcagcag ccgttgaatc cgtagggacg gatcggtgac
      841 tgcccatgtg gcaagcttgc gttgcatttc gctgattatc aaaggtcttc acctcgttag
      901 gtcagttaat tcacgtcgca aacacattca aactgcttcc cttcgccatg taatgggctt
      961 tccccatcgc ggactactac ggaagctccg ccagccagcg cgtcatcgga gccatgcccc
     1021 cttaacatcc gtcgctgacc ttccccggtt tacctgcctg gactcaggca tactgaggag
     1081 gctgcccgtc gcactcttta tccttgcttg ccgcaagttg gcagaagtca gcaacgcaag
     1141 cgtgatagac gctgctgccc cagtgtttcg catacatgtc aaaacacctt cgaccggcag
     1201 tgcttacgta tcactgccag ttcctcctgc acggcctgtc agatcacgta ggccgtggtg
     1261 acgttttcaa cccacagagg cggattaacg ggttcatgtt cttcagcctt tcagtactta
     1321 accttgagga tcatctcggc ttagtgatct cgcctcaatc cccgttgtca gcgggttaca
     1381 tcaccctgcg ggcatgccgc aggtcactgc cgctcaggtt ctccaccgtc acacccggtg
     1441 ggattgttgg gtttctcatc gtgagttacc ggttcaatat tccagacaga ctcgcggttc
     1501 atttaagcat ccatgcccgc cctgaactcc gggcacaccg taagtaaaat cagccaccca
     1561 caactggtca ggacgttctg ccacaaactg acggtttacg cggtcgcctg cggcaacggc
     1621 tttccggctg acggtagtgc ggaccttttt accccggaga acatcggcaa gtcccataac
     1681 cgccatgagg cgcgccactg tacatctggc taccctgatt ccttcccgta acaactgacg
     1741 ccgggttcaa cgagctgtac cgggatggcc ggataacgga agccgcctgt tgggctcacg
     1801 cccgccgtaa aatccacgat gtgcacgttc gcaccccgtc agccctgacg gaggaagcgc
     1861 tgaaacggat cggcgaactg tacgccatcg aggcagagat aaggggaatg acggcggagc
     1921 agcgccttgc cgaacgtcag ttgaaaacga aaccgctgct gaaatccctg gaaagctggc
     1981 tgcgtgaaaa gatgaaaacc ctgtcgcgac actcagaact ggcgaaagcg ttcgcatacg
     2041 ccctgaacca gtggccggcg ctgacgtact atgcagatga tggctgggct gaggcggaca
     2101 ataacatcgc tgaaaatgcg ttgcggatgg tcagtctggg ccgcaaaaac tacctgttct
     2161 tcggttcgga tcatggagga gagcggggag cgctgctgta cagcctgatc gggacgtgca
     2221 aactgaatgg agtggagcca gaaagctacc tccgctatgt ccttgacgtc atagccgact
     2281 ggccgataaa ccgggtcggc gaactgctcc cctggcgcgt agcactgccg actgaataac
     2341 acatccccgt caatacggtt cttgctgcac gcttacaatt
//
LOCUS       DAPHDW010000266         2363 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.272, whole genome shotgun sequence.
ACCESSION   DAPHDW010000266 DAPHDW010000000
VERSION     DAPHDW010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2363
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.272"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            80..1309
                     /locus_tag="RH057_004467"
     CDS             80..1309
                     /locus_tag="RH057_004467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HDV3130703.1"
                     /translation="MSYPVQKKIKTGENVLRAAVERINWVFDTFAEICLSFSGGKDST
                     VLFHLVADIARRKKRRFSVLFIDWEAQYLCTVRHVQKMRELYMDVTESFYWVALPLTT
                     VNGVSQFQPEWICWESGVEWVRQPPDYAVTDPNFFPFYQYAMTFEEFIPAFSSWMARS
                     KRGLAVLTGVRADESLNRFMGLASQHKLRYADDKPWTTASQDGFYYTMYPLYDWKTRD
                     IWIYNAKTHAIYNPLYDLMFRAGIPLRNMRVCEPFGPEQRKGLWLYHVLEPETWARMC
                     ARVSGAASGALYANESGAYFALRKRISKPDHHTWRSYAMFLLNVMPEKTAEHYRNKIA
                     VYLHWYQTHGYPDDIPDEQDNDLGSRDVPSWRRICKTLIKNDFWCRTLSFSPNKPRHY
                     ERYLQRMKERRKEWGIL"
     gene            1294..1932
                     /locus_tag="RH057_004468"
     CDS             1294..1932
                     /locus_tag="RH057_004468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HDV3130704.1"
                     /translation="MGDPLTPEILTVMIRQYFCQHLSEEETINALNHLRRVLHEISPF
                     AREPVDCVLWVKSDEVIANDYNPNVMAPGEKKLLKQSLEQDGFTQPVVVSEEKDHYLV
                     VDGYHRQLLGRGAGTEKRLRGWLPVTCINPERKGQATRMAATIRHNRARGKHQITSMS
                     DIVRDLSRLGWSDERIGTELGMDQDEVLRLKQISGLTELFQDEDFSPAWTVR"
     gene            1917..2273
                     /locus_tag="RH057_004469"
     CDS             1917..2273
                     /locus_tag="RH057_004469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HDV3130705.1"
                     /translation="MDGSLIQLNKILVDEFLSTQKRALEAVDDLIALKLEAAGCWRRA
                     SARWLVVMGAGDITDAQREWLLRRRAYCMAQTTSHVLHEKMNIRGVAKAADETLKRMG
                     IADLSEEMFRKRPSYY"
BASE COUNT          624 a          473 c          651 g          615 t
ORIGIN      
        1 gcactgtgct tgattacatg gttaccagag gtgtctctgt gtaagtgatg tttctgtatg
       61 tttattacat tgagaacaaa tgagttatcc ggtacagaaa aaaataaaaa caggtgaaaa
      121 tgttctccgg gctgctgtgg aacggattaa ctgggttttt gatacttttg cggagatttg
      181 tctgtctttt tccggaggta aggattccac tgttttattt catctggttg cggatattgc
      241 ccgcagaaaa aaacgacgtt tttccgtgtt gtttatcgac tgggaagcgc agtatttatg
      301 tactgtccgc catgtacaaa aaatgcgtga attgtatatg gatgtcacag agtcttttta
      361 ctgggtggcc ctgcctctga caacggtgaa tggggtttcc cagttccagc ctgaatggat
      421 atgctgggag tctggagtgg aatgggtccg tcagccacct gattatgctg ttactgaccc
      481 gaactttttc cctttttatc aatatgccat gacatttgaa gaattcattc cggctttttc
      541 atcttggatg gccagaagta aacgtgggct ggcagtgctg accggagttc gtgcagatga
      601 gtccctgaac aggtttatgg ggctggcttc gcagcataag ctacgctatg cggatgataa
      661 accatggacg actgcatcac aggacggttt ttactacacg atgtaccctc tgtatgactg
      721 gaaaacccgg gatatatgga tatacaacgc gaaaacgcat gccatttata accccctgta
      781 tgacctgatg ttcagagctg gcattccatt acggaacatg cgtgtctgtg aaccctttgg
      841 ccctgaacag cgtaagggat tatggcttta ccatgttctg gagcctgaaa cctgggccag
      901 aatgtgtgcc cgggtctcag gagcagccag tggagcgctg tatgcaaatg aaagtggtgc
      961 ttattttgcg cttcgaaaac gtatatcaaa gcctgaccat catacctggc gcagctatgc
     1021 gatgttttta ctgaatgtga tgccggaaaa aacggcggaa cattaccgga acaaaatagc
     1081 cgtatatttg cactggtacc agacacatgg atatcccgat gatattcctg atgagcagga
     1141 taatgatctg gggagtcgtg atgttccgtc atggcggcgt atatgtaaga cgctcataaa
     1201 gaatgatttc tggtgccgga ccctgtcctt cagcccaaac aaaccccgtc actatgaacg
     1261 ttatctgcaa agaatgaaag agaggagaaa ggaatggggg atcctttaac gccggaaata
     1321 ctgacagtca tgatccgtca gtatttctgt cagcacctgt cagaagaaga gacaattaat
     1381 gcgctgaatc atctgcgcag ggtgcttcat gagatcagcc cttttgcccg ggaaccggtg
     1441 gactgtgtgt tatgggtgaa atcggatgag gtgatagcca acgattataa tcccaatgtc
     1501 atggcgccgg gtgagaagaa actgctgaag cagtcactgg agcaggatgg gtttacacaa
     1561 ccagtcgtgg tatcggaaga aaaagatcat tatctggtgg tggatggtta tcatcgtcag
     1621 ttgctgggaa gaggggccgg tacagaaaaa cgcctccggg gatggcttcc tgttacctgc
     1681 attaatccgg agagaaaggg gcaggcgacc cgtatggccg cgaccatccg tcataaccgg
     1741 gccagaggaa agcatcagat tacatcaatg tcagatatcg ttcgtgacct gtcccgtctg
     1801 ggatggtcgg atgaaagaat tggtactgag ctgggtatgg accaggatga agtgctgagg
     1861 ctgaaacaaa tcagcggact gactgagctg tttcaggatg aagatttcag tccggcatgg
     1921 acggttcgtt aattcaatta aataaaattc tggtggatga gtttttgagt acgcagaaga
     1981 gagcactgga agcagtggat gatctgatcg cactgaagct tgaggctgca ggatgctggc
     2041 gcagggcttc tgcacgatgg ctggtcgtga tgggggcggg cgatattaca gatgcacaac
     2101 gggagtggct tttacgacga cgggcatatt gcatggcgca gaccacttct catgttttac
     2161 acgaaaagat gaatatccgg ggggtggcaa aagctgcaga tgagaccctg aagcgtatgg
     2221 gaattgcaga tctgtcagaa gagatgttca gaaaaagacc atcatactac tgataacggg
     2281 ctctctgtgg ctggcttctg aagtggaata tatgtgaaag agaccggata tattgcgctt
     2341 tttactttca ataaatcagt aaa
//
LOCUS       DAPHDW010000267         2325 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.273, whole genome shotgun sequence.
ACCESSION   DAPHDW010000267 DAPHDW010000000
VERSION     DAPHDW010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.273"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            95..598
                     /locus_tag="RH057_004470"
     CDS             95..598
                     /locus_tag="RH057_004470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3130706.1"
                     /translation="MNNIAPQSPVMRRLTQQDNPAIARVIRQVSAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYDGEVVGGGGIAPLTGSESDICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIGLYEHLGFQHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(644..1060)
                     /gene="rraB"
                     /locus_tag="RH057_004471"
     CDS             complement(644..1060)
                     /gene="rraB"
                     /locus_tag="RH057_004471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HDV3130707.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            1222..2226
                     /gene="argF"
                     /locus_tag="RH057_004472"
     CDS             1222..2226
                     /gene="argF"
                     /locus_tag="RH057_004472"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HDV3130708.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRTLAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     "
BASE COUNT          599 a          549 c          577 g          600 t
ORIGIN      
        1 caaaaacttt attactttat aagtcgcttg cgcacgtaag aagagagtgg tattaaacaa
       61 atgtataatt actttatttt tattgggtta aactatgaat aacattgcgc cgcaatcacc
      121 tgtaatgcgt cgcctgacgc agcaggataa tcccgccatc gcccgcgtca ttcgtcaggt
      181 atccgccgaa tacggtctta ccgctgataa aggctacacc gtcgccgatc cgaacctgga
      241 cgagttgtat caagtgtata gtcagccggg tcatgcatat tgggtggttg agtacgatgg
      301 tgaagtggtc ggcggcggtg ggattgcgcc attaaccggg agtgagtcgg acatttgcga
      361 actgcagaag atgtattttc ttccggctat tcgcggtaaa gggctggcga aaaaactggc
      421 cttaatggcg atggagcagg cacgagagat gggcttcaaa cgctgctatc tggaaacgac
      481 cgcttttttg aaagaagcga ttggtttgta tgaacattta ggttttcaac atatcgacta
      541 tgcacttggc tgcacgggcc atgtagattg tgaagtgcgg atgctacgcg aactctaaaa
      601 aaatgcccgc tagtgtgtgc tatcgggcat tttaatcaat taattagtgg cgaaccccgt
      661 catcgtcttc atcgacaaaa tcttcatcgt cgccgtcttc gccattggga tcttcaaagt
      721 aagtgcccca accgtcgtac tcaacgtcaa atttctccgc cagcgtcatc agttgttcaa
      781 cctgggcatc gatcagatcg gcattcaacg cgcactcgct gaggatgtcg cagcaaatca
      841 cgatatcacc atcttcaact tccagctctt ctggatcggt cacttcgtaa ccgagtttaa
      901 acgcttcaac tgctgctttt tccagggttt ccagatcgtc tgcggaaaga tggtgttcga
      961 tggtgtacag cgcgtccgga tcgctgccat cttccagtaa ttcttcaata atcaaacgtg
     1021 tttcttcacg ctgttcttcc agttgttccg ggtttgccat ggctcattcc tcttaatgtc
     1081 ctgccgatac tcttattgtc acacaccact gacattgcct ccacctttgt gacaaagatt
     1141 tatgctttag acttgcaaat gaataatcat ccatataaat tgaattttaa ttcattaagg
     1201 cgttagccac aggagggatc tatgtccggg ttttatcata agcatttcct gaaattactc
     1261 gatttcacgc cagctgaact caacagcctg ctgcagttag ccgcgaagct gaaagccgat
     1321 aagaaaagcg gcaaagaaga agccaaactc actggtaaaa acatcgcgct catcttcgaa
     1381 aaagactcga cccgtacccg atgctctttc gaagttgccg catatgacca gggtgctcgc
     1441 gttacttatc tcggcccaag cggcagtcag attggtcata aagagtcgat taaagacact
     1501 gcccgcgtgc tgggtcgcat gtatgacggt attcagtatc gcggctatgg tcaggagatt
     1561 gtcgaaacac tggcggaata cgctggcgtg ccggtatgga acggcctgac caatgagttt
     1621 caccccacgc agttgctggc ggatctcctc accatgcaag agcatttgcc cggtaaagcg
     1681 ttcaacgaaa tgacgctggt ctatgcaggt gacgcacgta acaacatggg caattcgatg
     1741 ctcgaagctg cggcgcttac cggtctggat ttgcgtctgg tcgcgccaca ggcatgctgg
     1801 ccggaagctg cgctggttac ggaatgccgc accctggcac agcaaaatgg cgggaatatt
     1861 acgctgacgg aagatgtagc caaaggagtt gaaggtgctg actttatcta taccgatgta
     1921 tgggtgtcga tgggggaagc aaaagagaaa tgggcagaac ggattgcatt actgcgtgat
     1981 tatcaggtga acagcaagat gatgcagttg accggtaacc cggaggtcaa attcctccac
     2041 tgcctgcccg cgtttcatga cgaccagacg acgcttggta agaaaatggc ggaagagttt
     2101 ggcctacatg gcggaatgga agtgactgat gaggtcttcg aatctgccgc cagcattgtt
     2161 tttgatcagg cggaaaaccg catgcatacc atcaaagcgg tgatggtcgc gacgctcagt
     2221 aaataattga tatcgctata aagatgccgg gaaatacaaa aacatcccgg catcacatta
     2281 ttttatgcag taatagttca attattccat ccatattttt ctatt
//
LOCUS       DAPHDW010000268         2304 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.274, whole genome shotgun sequence.
ACCESSION   DAPHDW010000268 DAPHDW010000000
VERSION     DAPHDW010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2304
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.274"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            176..745
                     /locus_tag="RH057_004473"
     CDS             176..745
                     /locus_tag="RH057_004473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
                     /protein_id="HDV3130709.1"
                     /translation="MTYKYNPLWQQRIRETVRHALDVHPRLTALRVDLRLPDVPAATD
                     AAVISRFINALKARIDAYQKRKHREGKRVHSSTLHYAWAREFGELKGKKHYHLLLLVN
                     RDTWCRAGDYRAPGSLAGMIKQAWCSALGVDAGCYATLVHFPVRPAVWLERDDETGFQ
                     QVLERAGYLAKEHTKARGTGERNFGCSRG"
     gene            912..1238
                     /locus_tag="RH057_004474"
     CDS             912..1238
                     /locus_tag="RH057_004474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130710.1"
                     /translation="MYAKSFLALDGNGRLTGARTAQTAPYDRYTCHLCGSTLRYHPQY
                     DTELPWFEHTDEGLTKHGHECPYVRPECREIQLIKRLQKFVPDALPVVRKASWHCTKQ
                     GIGSCR"
     gene            1204..1488
                     /locus_tag="RH057_004475"
                     /pseudo
     CDS             1204..1488
                     /locus_tag="RH057_004475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842637.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1494..1868
                     /locus_tag="RH057_004476"
     CDS             1494..1868
                     /locus_tag="RH057_004476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130711.1"
                     /translation="MTLFDECREALSADFNIVEGLAQQEAMGILNKYPLAKGRVTWSE
                     IRHSDYESFDELLSANSVKNDDMFVFADDASIPVFRSNLRLIAENIYDVTALSPKLFI
                     FNDEVIIQPLFPTDMFRLGIKK"
     gene            2015..2304
                     /gene="tnpA"
                     /locus_tag="RH057_004477"
                     /pseudo
     CDS             2015..2304
                     /gene="tnpA"
                     /locus_tag="RH057_004477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT          614 a          518 c          615 g          557 t
ORIGIN      
        1 ccggcttttt gcattaaaaa aacaacgctg aaaattcagc cgtcagtccg gagacgacag
       61 tcgggttatc tcagagtgcc tgagatattg cctggttgga gatcacttca gggactctgc
      121 gcatttttta cggcgtggtt aatccgtatg aacgaaacca tacggagatt taaccatgac
      181 ctacaaatac aaccccctct ggcagcaacg tattcgtgag acggtgcggc acgcactgga
      241 tgttcatccc cgtctgacgg cattgcgggt tgatctgcgt ctcccggatg taccggcagc
      301 aacggacgcg gctgtgatat cccgcttcat caatgccctg aaagcccgca tcgacgctta
      361 ccagaaacgt aagcatcggg aaggtaaacg cgtgcattcc tcgaccctgc attacgcctg
      421 ggcccgggag ttcggggagc ttaaaggcaa aaaacactat cacctgctgc tgctggtcaa
      481 ccgggatacc tggtgtcgtg ccggcgatta ccgcgctccg gggtcactgg ccggaatgat
      541 taaacaggcc tggtgcagtg ccctgggggt ggatgccgga tgttatgcca cgctggtgca
      601 ttttccggtc aggccggcgg tgtggctgga acgcgatgat gaaaccggct ttcagcaggt
      661 gctggaacgt gctggctatc tggcgaagga gcataccaaa gctcgcggca ccggtgagcg
      721 caactttggc tgcagtcgtg gctgagcccg acagacgact tatctgacct tagtgtgatg
      781 acgcgttcac agcgaccaca ctgattttcc cttctgagac tgaactgaaa accgctattc
      841 acacctgacc acgctgcccg taccgggacg gctgttgcct gcgtgtcgtc tggcggctga
      901 ggaatatgac gatgtacgca aaatcctttc tcgcacttga tggcaacggg cgtctgactg
      961 gtgcccgtac cgcacagact gcaccttacg atcgctacac ttgccacctt tgcggcagta
     1021 cactcagata ccatccgcaa tacgacactg aacttccctg gtttgaacac actgacgaag
     1081 ggctgactaa gcacggtcat gagtgccctt atgtcagacc ggagtgcaga gaaatacaac
     1141 tgattaagcg tctgcagaaa ttcgtaccgg atgccttacc cgtggtgcgt aaagccagct
     1201 ggcactgcac gaaacaaggc attggatctt gtaggtaatt taggagcgga ctctaagcca
     1261 gttatcggac gtttggaagt cagcgctggt aatgggaaag tcatagggcg gcaatccagc
     1321 gacggaaaag ttggttggcg tgttgattat gatcctgaaa aaggcacaca catcaatata
     1381 tgggattact cacagggtaa aggtcctgga aaagcggtga agcaagtcat accttttgaa
     1441 ggcaatgaaa agtcttttga aacaaaattg aaacagctaa acaggtagag taaatgactt
     1501 tatttgatga gtgtcgggaa gcactgagtg ctgattttaa tatagtagag gggctggctc
     1561 agcaggaggc tatgggcatt ttaaataagt atcctctcgc gaaaggacgt gttacttggt
     1621 cagagatcag gcattctgat tatgagagtt ttgatgagtt attgagtgca aactctgtaa
     1681 aaaacgatga catgtttgtc tttgcagatg atgcaagtat tccagttttt aggtcaaatc
     1741 taagattaat cgctgaaaat atctatgacg taacagcttt atcgcccaaa ttattcattt
     1801 ttaatgatga agtgataata caacctttat tcccaactga tatgttccgt ttaggaatca
     1861 agaaatagtg ggaaagctcg caaatcccgg ctaggctggg ggttccggaa agctttcagc
     1921 tttgagccag ttattaaaac cccttttgat ttgttaaaac accttgcggt ctggcaactg
     1981 caagtgtcaa acatgaaatc aaaagggggt cccaatgggg aacgaaaaga gtttagcgca
     2041 cacccgatgg aactgtaaat atcacatagt ttttgcgcca aaataccgaa gactggtgtt
     2101 ctacagagag aagcgtagag caacaggcag tattttgaga aagctgtgtg agtggaaaag
     2161 cgtacggatt cttgaagctg aatgctgtgc agatcatgtc catatgattg tggagacccg
     2221 cccaaaatga gcgtatccgg ctttatggga tatctgaaag ggaaaagcag tccgatgctt
     2281 tacgagcagt ctggggattt gaaa
//
LOCUS       DAPHDW010000269         2228 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.275, whole genome shotgun sequence.
ACCESSION   DAPHDW010000269 DAPHDW010000000
VERSION     DAPHDW010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2228)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2228)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2228
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.275"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..447
                     /locus_tag="RH057_004478"
     CDS             <1..447
                     /locus_tag="RH057_004478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012822426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130712.1"
                     /translation="DDVYIANCIEKGKTEFKVSKDIAALPFKGEPIEKIRGELIRSIK
                     EHLISASWHSTKKVIDRYEATFDIKVQKDCPIEAIELATLNRNHLVHRGGKDKEGNLV
                     VITDQDLETLIENASNLAIMLYNSLNVATNKTTILQPDDKPFIHEF"
     gene            463..1464
                     /locus_tag="RH057_004479"
     CDS             463..1464
                     /locus_tag="RH057_004479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013811181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130713.1"
                     /translation="MDIRTRKTKFLESLDSTEVIRKAVSLAIDCIIDNHNSNEDIPLV
                     ITSYDDLCRIQVLNYVQEFCEAAFPDMDEYYFSPNILRINGKTSEEACINLIKLLRST
                     KGMLFWSDAPSWFASLPDGLFHVVNIDQKIVTRGLNKKNSKPTIINKDYSVDTLLSEL
                     FLNGAHMEQPNVHNVSEGNMKFYDECHAGLIRPIPAPIGASYDEEITINSPDWQKLAC
                     VALRRYQSKECHDGMQWDTTDHGWTDVIAYPFVEEIQSMDNSGYRQCLVGLVTINNSN
                     ANSPYLSTVWIHPFYRRRGLLSKLWPKLQELYGSNFEIERPNENMKAFLKSAKYADY"
     gene            2082..>2228
                     /gene="hokD"
                     /locus_tag="RH057_004480"
     CDS             2082..>2228
                     /gene="hokD"
                     /locus_tag="RH057_004480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HDV3130714.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRLRTGQTEVAVF
                     TAYEP"
BASE COUNT          708 a          434 c          496 g          590 t
ORIGIN      
        1 gatgatgtct acatagccaa ttgtatagaa aaaggtaaga ctgaatttaa agtaagcaaa
       61 gatattgctg cgttgccttt caagggtgaa cccatcgaga aaattagggg agagctaatc
      121 aggtcaatca aggaacactt gatcagtgca agctggcata gcactaaaaa ggtaatcgac
      181 cgctatgaag ctacattcga tataaaagta caaaaagact gtccgataga agctattgaa
      241 ttggcaaccc tcaatcgaaa tcacttagtg catcgtggtg gaaaggataa agaagggaac
      301 ctcgtagtga ttacagacca ggatcttgaa acgctgattg aaaatgcatc gaacctggca
      361 attatgctct acaacagttt aaatgtagca acgaataaaa ccaccatttt gcaaccagat
      421 gacaaaccgt tcattcatga attttaatag cgcaggtcat acatggatat tcgcacacga
      481 aagacaaaat ttttagagtc gttagattca actgaagtga tacgcaaagc agtaagtctc
      541 gctatagatt gcattattga taatcataat agtaatgaag atatcccctt agttattacc
      601 agctatgatg atctctgtag aatccaggtg ctaaattatg tacaggagtt ttgcgaagct
      661 gcattccctg atatggatga atattacttt agtcctaaca tacttcgcat caacggcaag
      721 actagtgaag aagcttgtat taacttaatt aaactcttaa gaagtacaaa aggcatgcta
      781 ttctggtccg atgccccatc gtggttcgct agtctccccg acggattatt ccatgttgtt
      841 aacattgatc aaaaaatcgt tactcgcggc cttaataaga aaaactctaa gccgacaata
      901 atcaataaag attacagtgt agatacacta ctatcagagc tatttttgaa tggcgcacac
      961 atggagcaac ccaacgtaca caacgtttct gagggaaaca tgaagttcta tgatgaatgc
     1021 catgctggat taatcagacc aatacccgct ccaataggcg cgtcatacga tgaagagatc
     1081 acaattaatt ctccagattg gcaaaagctc gcgtgtgtag ctttacgccg ctatcagtcg
     1141 aaggaatgtc atgacggaat gcaatgggat acaaccgatc atggttggac tgatgtaatc
     1201 gcgtatccat tcgtcgaaga aatacaatca atggataata gtggttatcg acaatgttta
     1261 gttgggctgg tcacaataaa caattcaaat gcaaattctc cgtacctatc taccgtttgg
     1321 atacatccat tttatcgcag gagagggtta ttaagtaagt tatggccaaa attacaagag
     1381 ttatatggaa gtaacttcga aattgagcga cctaatgaaa atatgaaagc atttctgaaa
     1441 agtgcgaaat acgcagatta ctaatttatg aatcagtctg tggagttagg gttggtgtca
     1501 acaagtaggg taagagcctc aacgtgatgc tatgccgacc tgctccctga gaattcacac
     1561 agaaggctgc tagcaatgtc tacagatcgc tcaaagcgga ctagaaggtt agcttgcgtc
     1621 ggacttggcg tatttaaaga agtgctggtg gtgactggtt gttgtgttcc atttctacag
     1681 aacaaaatca cagaaactat acccaatagt tatattgaat caatgatgag acagcctcat
     1741 atttatcagg actggtgtac gtccaataca ggaggttgtc gtgctggttc tcaaatgtgc
     1801 gctagctatt gcggctgtaa tggcaattta ttgtcttgct gttgttctta cggatcgcct
     1861 ttctgattga ttttatattg gcgaggtgac gtgagttaag tagaattgct gcgggtgctt
     1921 gaggctatct gcctcaggca tgaacaccaa aggcagatag agaaaagccc cagttaacat
     1981 tacgcgtcct gcaagacgct taacattaat ctgaggccaa tttcatgcta gacacatgta
     2041 ggttagcctc ttacgtgccg aaaggcaagg agaagcaggc tatgaagcag caaaaggcga
     2101 tgttaatcgc cctgatcgtc atctgtttaa ccgtcatagt gacggcactg gtaacgagga
     2161 aagacctctg cgaggtacga ctccgaaccg gccagacgga ggtcgctgtc ttcacagctt
     2221 acgaacct
//
LOCUS       DAPHDW010000270         2204 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.277, whole genome shotgun sequence.
ACCESSION   DAPHDW010000270 DAPHDW010000000
VERSION     DAPHDW010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2204)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2204)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2204
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.277"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..974)
                     /locus_tag="RH057_004481"
     CDS             complement(<1..974)
                     /locus_tag="RH057_004481"
                     /inference="COORDINATES: protein
                     motif:HMM:NF024418.1,HMM:TIGR04415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV3130715.1"
                     /translation="MNKIYNIVWNESSGMWVVTSELTRKGGQRHRKIKRTMLAGLIAG
                     LMLPAIPAMAQMYNDKTLEYSDSAMELSAGDTATNTTINGGEQYISSGGNATSTTINE
                     GVQIVFDGGTASTTTINGGYQEISSGGLATNTIIDGGDQYISSGGNAIDTTIENGYQT
                     VFSGGNATSTIINGGFQEVSSGGSATSAIINAGFQTLYDNSIASSTVINNGFQLISSG
                     GSSVDTTIQGGIQEVGEGGSASATTINDGFQILLSGGSATNTTINNGWQDISSGGSAT
                     QTIISGGIQTIYDGGSASEITINSGYQVISSGGSVTTTTIYRGGEQSIT"
     gene            complement(967..2187)
                     /locus_tag="RH057_004482"
     CDS             complement(967..2187)
                     /locus_tag="RH057_004482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter strand-loop-strand
                     O-heptosyltransferase"
                     /protein_id="HDV3130716.1"
                     /translation="MSKLINTYFITPPEKPTQSGPEGIRYDFNDGARVLLPEGKWHVR
                     LMDADSGNILFSCDADNGWVRSCKKYFIRFRIQVFHRGNDTPLMDETLNLKNQPVLIS
                     FPTGTLGDLLGWFPYAERFQTLHKCQLECTMAQEIIELLAPQYPQIQFSTPDNPHTIT
                     PYATYRVGLYFGGDTTNQPIDFRQVGFHRSAGYILGVDPRESPVRLNLSAPRSINEPY
                     VCIATQSTCQAKYWNNGTGWSEVVAHLKSLGYRVLCIDLHAHYGQGFVWNHIPWGAED
                     FTGKIPLQERVNLLHHASFFIGLSSGLSWLAWATHIPVVLISGFTLPVSEFYTPWRIF
                     SSHGCNGCWDDTSLNFDHKDFLWCPRHKNTPRQFECTRLITGKQVNGVIDKLHASLAE
                     QSSIGMNHSRWSNE"
BASE COUNT          605 a          538 c          445 g          616 t
ORIGIN      
        1 gtaatactct gctccccacc acggtaaatg gtcgtggtgg ttacactgcc accgcttgag
       61 atgacctggt aaccactgtt gattgtaatc tccgacgctg agcctccatc gtaaatcgtc
      121 tggatacccc cgctaatgat cgtctgagtt gcagagccac cactggatat atcctgccaa
      181 ccattattaa tagtggtatt tgtggcactt cctccactga ggaggatctg aaaaccatca
      241 tttatagtcg tagcacttgc acttcccccc tcaccaactt cctgaattcc gccttgaata
      301 gtggtgtcaa cagaactgcc accactagaa atgagctgga agccattatt aatgacggtg
      361 ctactagcaa tactgttatc gtagagagtc tggaatccag cattaataat ggcactggta
      421 gcactgcctc cactggaaac ttcctggaac ccgccattaa taattgtact tgtagcgttg
      481 cctccactga aaactgtttg gtatccattt tcaatagtgg tatcaatagc attacctcca
      541 ctggagatat attgatctcc cccatcaatg atcgtattag tggctaggcc gccacttgag
      601 atttcttggt aaccaccatt gatggttgtc gttgaggctg tgccgccatc aaatactatc
      661 tgaactcctt cattaatagt cgtacttgta gcgttacctc cactggagat atattgttct
      721 cccccattaa tggtagtatt ggttgccgta tcgccagcgg ataactccat ggcggaatca
      781 ctatactcca gagttttatc attatacatt tgtgccatag ccggtatggc cggcaacata
      841 agtcccgcaa ttagtccggc taacattgtt ctctttattt ttcgatgccg ttgcccacct
      901 ttgcgggtta gctcagaagt caccacccac ataccagaag attcgttcca tacaatatta
      961 tagattttat tcattactcc accttgaatg attcatacca atactacttt gttctgccag
     1021 agaggcatga agtttgtcga tgacgccgtt tacctgcttc cccgtaatca ggcgtgtaca
     1081 ttcaaattgt ctgggggtgt ttttatgtct cggacaccac agaaaatctt tatgatcaaa
     1141 attcagtgaa gtatcatccc agcatccgtt acatccatgg gaactaaata ttctccatgg
     1201 ggtataaaat tcactgacag gaagcgtaaa accacttatt agtacaacag ggatatgagt
     1261 tgcccatgcc agccaggaga gcccgctgga taaaccaata aaaaaactgg catgatgtaa
     1321 cagattcacc cgttcctgca gaggtatttt ccctgtaaag tcttctgctc cccacggaat
     1381 atggttccag acaaagccct gaccataatg agcatgcaga tcaatacaca gtacccgata
     1441 ccccagcgat ttgagatggg ctacaacctc actccagcca gtaccgttgt tccagtattt
     1501 cgcctgacaa gtggattgtg tcgcgataca tacataaggc tcattaatgc ttctgggggc
     1561 tgaaagatta agtcggactg gtgattctcg gggatccact cccagaatgt atccagcgga
     1621 acgatgaaaa ccgacctggc gaaaatcaat tggctggtta gttgtatcgc caccaaaata
     1681 cagtcctaca cgataagttg catatggcgt aatcgtatga ggattatctg gagtagaaaa
     1741 ttgaatctgc ggatattgcg gtgccagtag ttcaataatc tcctgtgcca tagtgcattc
     1801 cagttgacac ttatgcagcg tctgaaacct ttcagcataa ggaaaccatc cgagtaaatc
     1861 acctaatgta ccggtaggaa acgaaatcag cacaggctga ttttttaaat tcaacgtttc
     1921 atccattaag ggcgtgtcgt ttcccctatg gaatacctga atacgaaaac gaatgaagta
     1981 ttttttacag ctccttaccc agccattatc tgcatcgcag ctaaaaagaa tgttccccga
     2041 atcagcatcc atcagacgaa catgccattt cccctcaggg agcagtacac gcgccccatc
     2101 attaaaatca tatcggatac cctcaggtcc ggactgagtc ggtttctctg gcggagtaat
     2161 aaaatatgtg tttatcaatt tggacatatc tttatcatta aaat
//
LOCUS       DAPHDW010000271         2181 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.278, whole genome shotgun sequence.
ACCESSION   DAPHDW010000271 DAPHDW010000000
VERSION     DAPHDW010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.278"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..1224)
                     /locus_tag="RH057_004483"
                     /pseudo
     CDS             complement(1..1224)
                     /locus_tag="RH057_004483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
     gene            complement(1220..1876)
                     /gene="tssI"
                     /locus_tag="RH057_004484"
                     /pseudo
     CDS             complement(1220..1876)
                     /gene="tssI"
                     /locus_tag="RH057_004484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310087.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
BASE COUNT          423 a          629 c          620 g          509 t
ORIGIN      
        1 gctctcgacc agtggctcgc catgctgtat ccgcgtgtgg aacaccaggc ggtgctggct
       61 gtcgtagtgg tagtggtggg tccgctcgtc gtccgtccgg ataacacccg ccgggataag
      121 gtccgtcttc tccgtcagtc tgccgtattc atcatagtgg taaacatagt gcgcatcctc
      181 tgcgatgcgg ttatccggcc acgccgtgag ggtactgtcc gggtgcagct ccgggtccgg
      241 caggcggttt cccgccgggt cggtggcata cgggatgcgg atgtccaggt ccggtgcgag
      301 ggtacgcacg ctctccagcc tgcccgtggc gctgtagccg tattcccgcg tctgtcgcgg
      361 gccgctgatg cgcaccaggt cgccgttgtc attccacccg tagtcacggt catacaccag
      421 gctgttcagg tgctggctct gtaactggcc tgcgggggtg tatgtgctgg tcagtttata
      481 tgcggcatta ctgcctgcca tgctgccaaa gctgcgcacc gtctcccggt gcatgcggtc
      541 gcgcgtgtac tccagcagcg gcgtgtcgcc aagcttcatg cccgccagat aaccgctgcc
      601 gtaggtcagc cactccaccg gcggcaggct gtccggcgtg acgcggtttg ccagcccctg
      661 ttcgttgtag gcgtgtgttg tctcatgctg ccacagcagt tcccccgtct ccgggttctc
      721 caccgtctgc cgttcgccgg tcaggcggcc cttatcgtcg tagccgtagt ggacagcgac
      781 acggtggcct tcgctcaggt ggctgatgtc tgtcagccag ccgtggtcat catactgcca
      841 ctgctctgcc ggttcgccgt tcaccgtgcg gtgagtgata cggtccgatt catcgtagta
      901 ccagagggtg acaagcccct catcttcact ctgcgtgagt tttccggtca ggtcataatg
      961 ataacgttgc gtccgcccgt caaagccgcc ctgctgtacc aaccggtcca gcgcatcgta
     1021 gctgaagtcg ctgtggctgc cgttctcgtt agtcagactg atgacacgtc cggcagcatc
     1081 gtactccata ctgcgcgtca gcccgccctg cgtggtgctg accgcctttc cccacgcatc
     1141 gtactgtgtc tcgctccggt tgccgtccgg ggtgataacg gcagtcaggt cgcctgcggc
     1201 gttgtactca tactgcgttt tgtgcactgt agcggaactg gctgatgcag agggtgctca
     1261 cctcggtacg ggtgttcgga ttccagggga tctcaaagga ctccggcaga taacgcacgg
     1321 tgtcgcacag gaccaggctc tggtcggtac ttttttgcgc gtgctcctca taaaagaaga
     1381 tgccttcctc cgccgccatc cggcacagga aatcgtaatc ggtttcgccg tactggacac
     1441 aaaactcacg ggaaggatgc ggctcgctga acagcggact ccactcggtc acaccgtttt
     1501 ccttcaggat cgtagcgagg atgctttcga tgtcctcatt ctggaagata cggaagttct
     1561 ggcgcagccc tgtgcgccac agcggcgggc acaccttcat gctgtacagc atctggtttt
     1621 tgtcgttctc cccgagttca aaccaggtca ccacgccttt cacccggcgc tgtacttcat
     1681 cgccctgcca tatcgtcagg taggccattt tgtccagcac ctgcgcaaat tcaagggaga
     1741 gaaactgctg gctgaccagg gagagatcga gggaaaagag cgaagagagt aactggttca
     1801 gatgaaagga aaccaccgca aaagcatccg gcggcaggcc gtccacttcc agtgtgaaac
     1861 gtaatccggt tgacatagca acctccgtga aaatgaataa taatccgcac atattgcatg
     1921 tacaacatgt cgggcggatg agataagcga gtcgacatct ggcgttaccg gggaatataa
     1981 gaaaaatgtc atggttatgt gatgatttag tctgggattt tacttcagag aaacgggtgt
     2041 ctgtttagtc aacaaaataa tcacaaacgc aacggatggg ttctcgacgt tattcacgac
     2101 aggctcgtac attacagcga gtaataaaaa acctgatatg caaagtcaaa ccaatattca
     2161 taatgtcttt ttattcacaa c
//
LOCUS       DAPHDW010000272         2119 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.279, whole genome shotgun sequence.
ACCESSION   DAPHDW010000272 DAPHDW010000000
VERSION     DAPHDW010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.279"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            73..405
                     /gene="hdeA"
                     /locus_tag="RH057_004485"
     CDS             73..405
                     /gene="hdeA"
                     /locus_tag="RH057_004485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HDV3130717.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            521..847
                     /gene="hdeB"
                     /locus_tag="RH057_004486"
     CDS             521..847
                     /gene="hdeB"
                     /locus_tag="RH057_004486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HDV3130718.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            911..1558
                     /locus_tag="RH057_004487"
     CDS             911..1558
                     /locus_tag="RH057_004487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HDV3130719.1"
                     /translation="MTAEFIIRLILAAIACGAIGMERQMRGKGAGLRTHVLIGMGSAL
                     FMIVSKYGFADVLSLDHVGLDPSRIAAQVVTGVGFIGAGNILVRNQNIVGLTTAADIW
                     VTAAIGMVIGSGMYELGIYGSVMTLLVLEVFHQLTFRLMNKNYHLQLTLVNGNTVSML
                     DWFKQQKIKTDLVSLQENEDHEVVAIDIQLHATTSIEDLLRLLKGMAGVKGVSIS"
     gene            complement(1600..>2119)
                     /locus_tag="RH057_004488"
     CDS             complement(1600..>2119)
                     /locus_tag="RH057_004488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3130720.1"
                     /translation="ITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVIIDTL
                     MNNVFHSNFLTQIERLKPVHVIIFSPFNIKRCLGKVPVTFVPRTITIIDFVALINGSY
                     CSVPEAAVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNLKRIN
                     ELVRHQHIDYLV"
BASE COUNT          621 a          414 c          483 g          601 t
ORIGIN      
        1 aaaccaggtt ataacctcag tgtcgaaatt gattcgtgac ggctctttca ctttatagtt
       61 gaggatatta cgatgaaaaa agtattaggc gttattcttg gtggtctgct tcttctgcca
      121 gttgtgagca atgcagcgga tgcgcaaaaa gcagctgata acaaaaaacc ggtcaactcc
      181 tggacctgtg aagatttcct ggctgtggac gaatccttcc agccaactgc agttggtttt
      241 gctgaagcgc tgaacaacaa agataaacca gaagatgcgg ttttagatgt tcagggtatt
      301 gcaaccgtaa ccccagctat cgttcaggct tgtactcagg ataaacaagc caactttaaa
      361 gataaagtta aaggcgaatg ggacaaaatt aagaaagata tgtaattccg ggaatgcgtt
      421 acatcgtact tccttgcata ttgaacaggc cggaatatct tctttaaaag cagctattcc
      481 tcctgttcat atataatctc tatattgaat gggttacaaa atgaatattt catctctccg
      541 taaagcgttt atttttatgg gcgctgtagc ggctttgtca ctggtgaacg cacaatctgc
      601 gctggcagcc aatgaatccg ctaaagatat gacctgccag gaatttattg atctgaatcc
      661 aaaagcaatg accccggttg catggtggat gctgcatgaa gaaacagtat ataaaggtgg
      721 cgataccgtt actttaaatg aaaccgatct cactcaaatt cctaaagtga tcgaatactg
      781 taagaaaaac ccgcagaaaa atttgtatac cttcaaaaat caagcatcta atgacttgcc
      841 gaattaatga ggtacaagta aaaaggagta gcaagttgag ccatcttgct gctccttttt
      901 gcatttttat atgacagcag aatttattat acgtcttata ctcgcggcaa ttgcctgtgg
      961 cgctattggc atggaaaggc aaatgcgcgg caaaggagca gggttacgca cacatgtatt
     1021 aattggcatg ggaagcgccc tgtttatgat tgtttcgaaa tatggttttg ctgacgtgct
     1081 gtctttagat cacgtcggac tcgaccccag ccgtattgct gctcaggtgg tgacgggcgt
     1141 cgggtttatc ggtgcaggta acattctcgt tcgtaaccaa aatattgtag gtctgacgac
     1201 ggcagcggat atctgggtga ccgccgccat aggtatggtt attggcagcg gtatgtacga
     1261 actgggtatt tatggttcag tgatgacctt gttggtgctg gaagtcttcc atcaattaac
     1321 cttccgcctg atgaacaaaa attaccattt acagctgaca ttagtgaacg gtaatacggt
     1381 atcaatgctc gactggttta agcagcaaaa aataaaaacg gatttagttt cgttgcagga
     1441 aaatgaagat catgaagtgg tcgccattga tatccagctt catgccacaa cgtcgataga
     1501 agacctgttg cgattattaa aaggtatggc aggggtgaaa ggcgtttcaa ttagctaatc
     1561 tgtgtggcag cgtagccaga ctcaccgtaa gcctgaaatt cacaccagat aatcaatatg
     1621 ctgatggcgt accagctcat tgatccgctt aagattgagg atcatcatga tattgtgttt
     1681 atggcagtag aacgttttta gcgatatttt cagtttctcc agaatatctt ccgttgtcat
     1741 ttgattcgca atgcagctca gaacctgatg ttgcttgcgc gaaagtgaca cagccgcttc
     1801 aggcacagag cagtaactgc cattgatgag tgcgacaaaa tcaatgatag tgatagtccg
     1861 cggaacaaag gtcaccggca ctttccccag gcagcgttta atattaaagg gggagaaaat
     1921 aatcacatgg acaggtttta atcgttcaat ttgagtgaga aaattagaat gaaatacatt
     1981 attcattaat gtatcaataa tgacgaaggc attctgatgc aacattacgt cgatctcttc
     2041 ttcgttctgt atatgaacga cattaccttt actcagaatg tttttcatcg cggtgaagaa
     2101 catcgtatcc ctggtaatt
//
LOCUS       DAPHDW010000273         2106 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.280, whole genome shotgun sequence.
ACCESSION   DAPHDW010000273 DAPHDW010000000
VERSION     DAPHDW010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2106
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.280"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1393..2052)
                     /locus_tag="RH057_004489"
     CDS             complement(1393..2052)
                     /locus_tag="RH057_004489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130721.1"
                     /translation="MLFLLTQLDFSEGTSDLVEMLMLLALTAAIVTIGILPGAIHLNE
                     RRNGDKFTGFIVVLICIPVVITVFSMWYRPIPNMIINMVMSLSGISDWRTHQYYIENK
                     THSHSMFNGLIWNTRHYTEIPERFFITGVSTFTLGDIKLVCPTEITEARKESLKFTIN
                     DINEYEQKGKQLKAVAMKCIPFNKNDIHTWDSPLSEPIYYEKVKQTIDNSMLKILHTL
                     K"
BASE COUNT          591 a          459 c          518 g          538 t
ORIGIN      
        1 tgtaatggtt gcctccggcg cagtttgcgg tgatgccgtt cccgggaatg gattgccagt
       61 gcggggacag tggagcttaa ttccggtatt ggtggccttt ccgttgattt tgagccaaca
      121 gccctcctgt gactgacagg ctgcctcgtc attccatacg tatcttcagg tgaacagatg
      181 catgtctgag cgtactcacg cagccggccc ggcaaccgcg gtggtactac gctggctgcg
      241 agaaaataca gaaagcacca gaatacgctc tcctgttgct gatcaatcag gatctcatgc
      301 acctaccggg atctagggct agaactgatg aaaccgattc gcacctggct gagttaatgc
      361 tacaggccag ggcatcacta ccccctgacg agcactgtac tgtggtatgc agttacaggg
      421 ggcgtgtgca gccacacctg aaagcagtga gtcgttcacg gcattcaaaa acacgtgcct
      481 ttcgttcgcc gttcctgtga tggccggcat catttgctaa gccgttaccg acgttattcc
      541 ctccgggaca cgcctctgat tgaggctttg cccggagcaa tcgaatgaaa ataagataag
      601 tcaccgaaag ggaggatgta gtcaaccggg ggaacagtaa taagccgtcg tgcttttatc
      661 gtacaagtgt gttgcgtttt gcaccgaaaa tacgatagag gtcgtttatg gattccgcag
      721 ggagtgctga ttattattca ctggcagcag acagcgatgt gtgtgcaggc attcaggggg
      781 atgttgtcag cagaagggtg agtatagcag attgttgtta tacatggcgt atgaagtatt
      841 accagtcatg cggagtagtg tggtgagggg ttgtatgaga aatcgccacc ttcttgtcag
      901 agagggaacg ctaccatgaa agtcaaaagc gataacgcag accggtacga tcggtaggta
      961 aaactgtagc ggtgtgctgc cgacgaggtc agaataatct gcgcgttttt acgccagcgc
     1021 caccgacgaa cagcaggaga ttgaggggaa tttgacggcg ataaagacgt agaagagaaa
     1081 aacgggcaat ttgccgcatg cccacagaat cagtgagatc gttagcgaca gcaaaatgat
     1141 cggcagccag cggcggcctg caatatcggg atgaaggaga tcctacgata tcgatgctgc
     1201 acctgatatg tacgatcagt aaatagagta cgaaacagaa aacacgacga taataacgct
     1261 ggcagcggtg cctgtcaggc aaacgcccac acgcaataaa ataaatcaac catcgcaggg
     1321 ccgtgatcat ccgcatacac cgctgatgac gcgtctaaaa acgcgtcaat atgacttccc
     1381 cggaacatta cctcatttca gggtatggag aatttttaac atcgagttat ctatggtctg
     1441 tttgactttc tcgtaataga tcggttcaga taatggcgaa tcccaggtat gaatatcgtt
     1501 cttattaaaa ggaatgcatt tcatggctac tgcctttaac tgtttccctt tttgctcata
     1561 ctcattgata tcattaatgg taaatttcaa actttctttt cttgcttctg ttatttcagt
     1621 tgggcatacc agctttatat ctcctaaggt aaatgtactg accccggtga tgaaaaatct
     1681 ctcggggatt tctgtatagt gccgagtatt ccagattaag ccattaaaca tactatgaga
     1741 atgagtttta ttttcgatat aatactgatg cgttctccag tcactaatgc cagagagact
     1801 cattaccata ttaatgatca tatttgggat agggcgatac cacatactga atacagtaat
     1861 tacaactggt atgcatatta aaacaactat gaatccagtg aatttatcac catttctgcg
     1921 ctcattaaga tgtatggcac caggcagaat gccaatagta acaatagctg ctgttaaggc
     1981 aaggagcatt agcatttcaa ccaaatcact tgtcccctca ctaaaatcaa gttgggtaag
     2041 cagaaataac ataggtaata tttgaacgat cccgggagtc ataaattgca agggtaaaaa
     2101 acataa
//
LOCUS       DAPHDW010000274         2073 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.281, whole genome shotgun sequence.
ACCESSION   DAPHDW010000274 DAPHDW010000000
VERSION     DAPHDW010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.281"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>2073
                     /gene="rhsB"
                     /locus_tag="RH057_004490"
     CDS             <1..>2073
                     /gene="rhsB"
                     /locus_tag="RH057_004490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDV3130722.1"
                     /translation="AEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHD
                     PEYPENLPAAPLVRYGWTPRGELAAVYDRSNTQVRSFTYDDKYRGRMVAHRHTGRPEI
                     RYRYDSDGRVTEQLNPAGLSYTYQYEKDRITITDSLDRREVLHTQGEAGLKRVVKKEH
                     ADGSVTQSQFDAVGRLRAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHNQ
                     LTSATGPDGLELRREYDESGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTM
                     TWSRYGQLLSFTDCSGYQTRYDHDRFGQMTAVHREEGLSQYRAYDSRGQLIAVKDTQG
                     HETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAVRTTQGGLTRSMEYDAAGRVIRLT
                     SENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRL
                     THRTVKGETAERWRYDERGWLTDISHISEGHRVAVHYRYDEKGRLTGERQTVHHPQTE
                     ALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGWLAGMKLGDTPLVDFTRDRLH
                     RETLRSFGRYELTTAYTPAGQLQRQHLNSLQYDRDYTWNDNGELIRISSPRQTRSYSY
                     STTGRLTGVHTTAANLDIRIPYATDPAGNRLPDPELHPDSTLSMWPDNRIARDAHYLY
                     RYDRYGRLT"
BASE COUNT          481 a          653 c          629 g          310 t
ORIGIN      
        1 ggcagaagaa gcccggcagc aggccatttc cggcgggacg gaaccgtccg cttttcctga
       61 taccctgccg ggttacaccg aatatggccg ggacaacggc atccgtctgt ctgccgtgtg
      121 gctgacgcac gacccggaat acccggagaa tttacctgcc gcgccgctgg tgcgctatgg
      181 ctggacgccg cgcggcgaac tggcggcagt gtatgaccgc agcaatacac aggtgcgcag
      241 ctttacttac gatgataaat accggggccg gatggtggcg caccgtcaca cgggccggcc
      301 ggaaatccgt taccgttacg acagcgacgg gcgggtgaca gaacagctaa acccggcagg
      361 cttaagctac acgtatcagt atgagaaaga ccgcatcacc atcaccgaca gcctggaccg
      421 ccgtgaagtg ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca
      481 cgcggacggc agcgtcacgc agagtcagtt tgacgccgtg ggcaggctca gggcacagac
      541 ggatgccgca ggcaggacaa cagagtacag cccggatgtg gtgacgggcc tcatcacgcg
      601 cataaccacg ccggatggca gggcatcggc gttttactat aaccaccaca accagttaac
      661 gtcagccacc gggcctgacg ggctggaatt gcgccgggaa tatgatgaat ccggccgtct
      721 gattcaggaa accgcccctg acggcgatat cacccgctac cgttatgata atccacacag
      781 tgacctcccc tgcgcaacgg acgatgccac cggcagccgg aaaaccatga cgtggagccg
      841 ttacggccag ttgctgagct tcaccgactg ttccggctac cagacccgtt atgaccatga
      901 ccgcttcggg cagatgacgg cggtgcaccg cgaggaaggg ctgagccagt accgcgcata
      961 cgacagccgt ggacagttaa ttgccgtgaa agacacgcag ggccatgaaa cgcggtatga
     1021 atacaacatc gccggtgacc tgaccgccgt cattgccccg gacggcagca gaaacgggac
     1081 acagtacgat gcgtggggaa aggccgtccg taccacgcag ggcgggctga cgcgcagtat
     1141 ggaatacgat gctgccggac gggtcatccg cctgaccagt gaaaacggca gccacaccac
     1201 cttccgttac gatgtacttg accggctgat acaggaaacc ggctttgacg gccgcacaca
     1261 gcgttatcac cacgacctga ccggcaaact tatccgcagc gaggatgagg gtctggtcac
     1321 ccactggcac tatgacgaag cagaccgcct cacgcaccgc accgtgaagg gtgaaaccgc
     1381 agagcggtgg cggtatgacg aacgtggctg gctgacagac atcagccata tcagcgaagg
     1441 gcaccgggta gcggtgcatt acaggtatga tgagaaaggc cgcctgaccg gtgagcgcca
     1501 gacggtgcat cacccgcaga cggaagcact gctctggcag catgagacca gacatgcgta
     1561 caacgcgcag ggactggcga accgctgtat accggacagc ctgcccgccg tggaatggct
     1621 gacctacggc agcggctggc tggcaggcat gaagctcggc gacacaccgc tggtggattt
     1681 cacccgcgac cgcctgcacc gggaaacgct gcgcagcttc ggtcgttatg aactcaccac
     1741 cgcttacacc cctgccgggc agttacagcg tcagcacctg aacagcctgc agtatgaccg
     1801 cgattacacc tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg
     1861 gagttacagc tacagcacca ccggcaggct gaccggcgtt cacaccaccg cagcgaatct
     1921 ggatatccgc atcccgtatg ccacggaccc ggcgggcaac cgcctgcccg acccggagct
     1981 gcacccggac agcaccctca gcatgtggcc ggataaccgt atcgcccgtg acgcgcacta
     2041 tctttaccgg tatgaccgtt acggcaggct gac
//
LOCUS       DAPHDW010000275         2071 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.282, whole genome shotgun sequence.
ACCESSION   DAPHDW010000275 DAPHDW010000000
VERSION     DAPHDW010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.282"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(31..>2071)
                     /locus_tag="RH057_004491"
     CDS             complement(31..>2071)
                     /locus_tag="RH057_004491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3130723.1"
                     /translation="VSGTLINNGEQRVSSGGSAIDTTINNGGTMSIAAGGSGTGIIQN
                     NGGAIRANTSAILSGTNVNGSFSITNGSARNMLLENGGLLMVLQGHQAIDTQVSKGGE
                     LQVESGSELLGTTLIKDDGKLSGNQITNNGVLHFLDNSSANYSGTLDGTGSLNLNGGK
                     LTLSGNFSQDRGIFIQNHGTMIMDNLRAMANVTIEPDATLRLEDGSLLAGRITGDGKK
                     GGNLIVADSVWKLSGDSTINSLDMSNGTVDFHSIQSPQFKTKLEGVTLNIGQLSGNGT
                     FLMNTDIASYTGDKLNITGDANGNFVLNIKNTGQEPVSPGNPLPVVYTGGGDAVFTVN
                     GGKVDAGVWEYYLSKDNTNWYLKSDTTQPGNPEPGIPNPPVRHTTKSTDAVLNMASAP
                     TYIFNSELQGLRFRNSEKSENSRTSGGVWGHYIGSDKHISSKGAGYSLTQYGIVTGGD
                     KRFDLSDGVLSLGAFVSFSDNNIRHNRGGKSSIDSKSVGLYMTYDTHGYYIDGVLKFN
                     RFKNELRTHMSDGTMVSGDYHQNGFGGSLEVGKYFSLNENTWLLPYARTTALAVNDNN
                     ISLSNGMKAEINTTHSFQGEVGVNLGMDLDVGKTKIRPYLTAGVSHEFSNHNKVKINK
                     AYDFTNDISGTTGKYGLGMDVQLTSNASIWGEANYLKGNNVESPVTGTVGFKINF"
BASE COUNT          544 a          509 c          372 g          646 t
ORIGIN      
        1 tatggtcggg catctgatgc acatcaggcg ttaaaagttt atcttgaagc caacagtccc
       61 tgttaccggt gactctacat tgttaccttt taaataatta gcctctcccc atatactggc
      121 atttgatgtt agctgaacat ccatacccaa accgtacttt ccggttgtcc cggatatgtc
      181 gttagtaaaa tcataggctt tattgatttt aaccttatta tgatttgaaa attcatgact
      241 gactcctgcg gtcaggtagg gccttatttt ggttttacca acatcaaggt ccatccctaa
      301 attaacacca acctcaccct ggaaagaatg tgttgtgttt atttcagcct tcattccatt
      361 agatagactg atattgttat cattcacagc aagagctgtt gttcgggcat aaggaagtaa
      421 ccaggtattc tcattcagag aaaaatactt gcccacctcc aggctaccac cgaaaccatt
      481 ttgatgataa tctcccgaaa ccatagttcc atcactcata tgggtacgca actcattttt
      541 gaaacggttg aatttgagta caccatctat atagtatccg tgagtatcat acgtcatgta
      601 caagccaaca cttttactat caatagaact tttccctcct cgattatgcc tgatattgtt
      661 atcagagaag gaaacaaatg cccctaatga caacacccca tcgcttaggt caaaacgctt
      721 atctccgccc gtcactattc cgtattgggt cagggagtaa cccgctcctt tgctgctgat
      781 gtgtttatct gatcctatat agtgccccca tactccccca gaagtacgtg agttttctga
      841 tttttcactg ttacggaatc gcagcccttg taattcactg ttaaagatat atgtcggagc
      901 gcttgccata ttcagtacag catcagtaga ctttgtggtg tgccgtactg gaggattggg
      961 tatacccggt tccggattac ctggttgtgt agtatcagat tttagatacc agttagtatt
     1021 atctttactc agataatatt cccaaactcc tgcatccact tttcctccgt ttactgtaaa
     1081 tacagcatct cctccaccgg tataaaccac tggtaatggg ttacccgggg atacaggctc
     1141 ctgaccggta tttttgatat tcaaaacaaa attaccgttt gcatcccctg tgatgtttaa
     1201 tttatcacca gtatatgatg caatatcagt attcatcaag aaggtgccat ttcctgataa
     1261 ctgtccgata tttaacgtta ctccctcaag ttttgtttta aactgagggg actgtattga
     1321 atgaaaatcc acggttccat tactcatgtc taatgagtta atagtagagt ctccggataa
     1381 tttccagaca gagtcagcca ctatcagatt tccacctttc ttcccatccc cagtaatgcg
     1441 tccagccagg aggctaccat cctctaagcg cagagttgca tcaggctcga ttgtcacatt
     1501 tgccatagcc cgtagattat ccattatcat tgttccatga ttttggataa aaatacctct
     1561 atcctgagaa aaattaccac tgagagttaa ttttccccca ttcagattca gactaccggt
     1621 accatccagt gtgccgctat agtttgctga actattgtcg agaaaatgca gaaccccatt
     1681 attggttatc tggttgcctg acagtttgcc atcgtcttta atcaaggttg tacccaatag
     1741 ctcagaaccg ctctcaacct gtaattcacc acctttagat acctgggtat ctatagcctg
     1801 atgaccctgc agaaccatca ggagtccacc attttctaac aacatatttc tggcgctacc
     1861 attagtaatg ctgaagctac catttacgtt cgttccactc aggatggccg atgtgttcgc
     1921 tctgattgcg ccaccattat tttgaattat tcccgttcca ctaccacctg cggcaatact
     1981 cattgtccct ccattgttaa tggttgtgtc aatagcactg ccaccactgg agactcgctg
     2041 ctccccgtta ttaatgagag tccctgagac a
//
LOCUS       DAPHDW010000276         2016 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.283, whole genome shotgun sequence.
ACCESSION   DAPHDW010000276 DAPHDW010000000
VERSION     DAPHDW010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.283"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            81..671
                     /locus_tag="RH057_004492"
     CDS             81..671
                     /locus_tag="RH057_004492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001545742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130724.1"
                     /translation="MTQSRRPSPLQRRVLIVLAALDEKRPGPVLTRDIERVLERSGEA
                     PVYGPNLRASCRRLEDAGWLRTLRAPNLQLAVELTDAGRAVAQPLLLAEQDRLRAEQR
                     AAEVVVLPLVPAAGLPADGTSATDLAVELNGITYQACRGDFVVRLDGSTCLQLWNKEG
                     RVVCLEGVPLEVAQWLQACHDAGIEVRVQINESSVP"
     gene            complement(689..1036)
                     /locus_tag="RH057_004493"
     CDS             complement(689..1036)
                     /locus_tag="RH057_004493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130725.1"
                     /translation="MPFEQKKARAIALMDSKKMWRSNYAPPLLRILWRLGIRLPPLPF
                     MPFWQVAVLTGGLWGTSWGCAMWFIYWGPSGMVAGEAIIISITGGFLFGLLTASFHWW
                     RRKVNRLPPWDDV"
     gene            complement(1121..1402)
                     /locus_tag="RH057_004494"
     CDS             complement(1121..1402)
                     /locus_tag="RH057_004494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HDV3130726.1"
                     /translation="MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLA
                     GQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKVYILRVLHTAQKWP"
     gene            complement(1399..1668)
                     /locus_tag="RH057_004495"
     CDS             complement(1399..1668)
                     /locus_tag="RH057_004495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130727.1"
                     /translation="MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQ
                     HNTWFRDQVAAGRQQLEHGDVLPHDMVESSAAAWRDEMSRKVAGK"
BASE COUNT          452 a          589 c          547 g          428 t
ORIGIN      
        1 aatcacgcac aggcccggga acgcatcgat accgattatc agcgcctcaa aaaactcatc
       61 tggtcggagg gtgacagtta atgactcaga gccgccgacc gtcgccactg cagcggagag
      121 tgctgattgt gctggctgcc cttgatgaaa aacggccggg acccgtgctg acgcgggaca
      181 tagaacgggt gctggagcga agcggggaag ccccggtcta cgggccaaac ctgcgcgcct
      241 cctgccgtcg tctggaggat gccggctggc ttcgtaccct gcgcgctccg aacctgcagc
      301 tggcagtgga gctgacggac gccggccggg ccgtggcaca gcctctgctg ctggcagaac
      361 aggaccgcct ccgggcagaa cagcgtgcgg cagaagtcgt cgtactgccg ctggtcccgg
      421 cagcaggtct gcccgcagac ggcacttctg ccactgatct ggcggtagag ctgaacggca
      481 taacgtatca ggcctgccgt ggggattttg tggtgcgtct cgacggcagc acctgtctgc
      541 agttatggaa taaagaaggc agggtggtat gcctggaggg cgttccgctg gaagtggcgc
      601 agtggctgca ggcctgtcat gacgcaggaa tagaggttcg cgtacagata aatgaaagta
      661 gcgtcccgta aaaacatgcc tgcatggttt acacgtcatc ccatggtggc agccggttaa
      721 ctttcctgcg ccaccagtgg aaagaggccg tgagcagtcc gaacaaaaaa ccacccgtga
      781 tacttataat aatggcctca cctgccacca ttcctgacgg cccccagtag ataaaccaca
      841 ttgcacatcc ccaggaagtg ccccataagc cccccgtcag caccgcgacc tgccagaacg
      901 gcataaaggg taatggcgga agccggatac ccagccgcca gagaatacgc agaagaggag
      961 gggcataatt gctccgccac atctttttgc tgtccatcag ggcaatggcc cgggcttttt
     1021 tctgctcaaa gggcacagga gcacctccgt tccatgatgt aaacgtgaca cagcttattg
     1081 ttattgatta ttatcatgct gtgatatccg gacagcccct ctatggccac ttctgtgcag
     1141 tatgcaatac tcgcaggata tacacttttc cccactggct gtcgacctca taaaccagaa
     1201 caaaatgtgg gtgaataacc agttcccggg taccttccac acgaccgttg cggcctttat
     1261 acggctgccc ggccaggctg ctggctgaat aaccaatccg ttcgtccagt tcaatggcgg
     1321 ccatcaggtt ctgctccgca atgtactcac gaatgcgctt cctgtcctgg ctggccagca
     1381 tggtccagaa tatctccatc atttaccggc gaccttcctg ctcatttcat ccctccatgc
     1441 tgcagcagat gattcgacca tgtcatgggg aagcacatcg ccgtgctcga gttgctgacg
     1501 tcctgccgca acctggtcgc ggaaccaggt attatgctgc cgttcaacgg tctggcgcat
     1561 aaaatcccgg attaactgag agccattacg atccatgctt tttgcagcgg ccataaaagc
     1621 atccttcagt tcagcgtcga ttcttaaact catattaacc tgtgccattt atcacctcgt
     1681 cattactacg tatatacaca gtatataacg attcagatat cgtcaccagg atatgccgcg
     1741 tcagcggcat ggaaggcggc actccattgt tgcatatgat accggagtaa aaccgccgaa
     1801 gcccggcgta agccggtact gattgataga tttcacctgt cccatccccg ccctgctaag
     1861 ccatacccgc tttcagccat gatagcgctt ctgtgcgcgg tcggagtggt cccgacgagg
     1921 gtttacccga agtcggggcg tatctccgcg ttagcgggcc gtcagggccg cttacgagcg
     1981 tgtactcgat acttccagcg agaagactga cagcga
//
LOCUS       DAPHDW010000277         2006 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.284, whole genome shotgun sequence.
ACCESSION   DAPHDW010000277 DAPHDW010000000
VERSION     DAPHDW010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2006)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2006)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2006
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.284"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            249..503
                     /gene="bssS"
                     /locus_tag="RH057_004496"
     CDS             249..503
                     /gene="bssS"
                     /locus_tag="RH057_004496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HDV3130728.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            618..1736
                     /gene="solA"
                     /locus_tag="RH057_004497"
     CDS             618..1736
                     /gene="solA"
                     /locus_tag="RH057_004497"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HDV3130729.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVVSDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDKKSDFDLTPFRLSRFQ"
     gene            1784..1897
                     /locus_tag="RH057_004498"
     CDS             1784..1897
                     /locus_tag="RH057_004498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HDV3130730.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
BASE COUNT          533 a          475 c          457 g          541 t
ORIGIN      
        1 ttattttact tacaaatttt gagtacaaaa tttctacact tcctcaaaat aattgcacaa
       61 tatgctaaaa aaatatgaat gacatgctgt tttattgttc atataaatct taaaagcaga
      121 aatttatctg ttcccccctt taaaaattcg gtgaataccc ttacttattg gtatactgaa
      181 gttgcttcag ataaaaacgc attgaacctc gaataacgtt gtctagtaac acgaattagg
      241 gggccatgat ggaaaaaaat aatgaagtca ttcagactca tccgctcgta gggtgggaca
      301 tcagcaccgt tgatagctat gatgcgctga tgttgcgttt gcactaccag accccaaata
      361 agtccgagca ggaagggact gaagttggtc agacgctctg gttaaccact gatgttgcca
      421 gacaatttat ttcgatatta gaagcaggaa tcgccaaaat tgaatccggt gatttcccgg
      481 taaacgagta tcggcgtcat taacatgaca tgaccatcca cccaaaaggc acctcaccgg
      541 tgcctttacc atttctgggt tgtatatctg cattcagtta attactctgc ttaaaccatg
      601 agagcagaga gaaaaaaatg aaatacgatc tcatcattat tggcagcggt tccgtaggcg
      661 ctgccgccgg gtattatgca acccgcgccg gtttaaacgt gctaatgacc gacgcccata
      721 tgccaccgca tcaacacggc agccaccacg gcgatacgcg attaattcgc catgcttatg
      781 gtgaaggcga aaagtatgtc ccgttggtcc tccgcgcgca aacgctgtgg gatgaacttt
      841 cccgccacaa cgaagatgat cccatttttg tacgctctgg tgtcattaac ctcggcccgg
      901 ctgactccgc atttctcgcc aacgtcgccc acagcgccga acagtggcaa ctcaacgttg
      961 aaaagctcga tgcgcaaggg attatggccc gctggccaga aatacgcgtc ccggacaact
     1021 acatcggctt atttgagact gattccggtt ttttgcgcag cgaactggcg attaaaacct
     1081 ggatccaact ggcgaaggaa gcgggctgtg cgcaactgtt caactgcccg gtcaccgcaa
     1141 ttcgtcatga cgatgatggc gtaactattg aaaccgttga cggtgagtat caggcgaaaa
     1201 aagcgattgt ctgcgcggga acatgggtaa aagacctgct cccggagctg cctgtccagc
     1261 ccgtacgtaa agtatttgcc tggtatcagg ccgatggccg ctatagcgtg aagaataaat
     1321 tcccggcgtt taccggtgaa ctgcccaatg gcgatcaata ttatggtttt ccggcagaaa
     1381 acgacgcgtt gaagattggc aaacataacg gaggccaggt tatccattca gcggatgaac
     1441 gtgttccgtt tgcggaagtg gtcagcgatg gttcggaagc cttcccgttc ttgcgcaatg
     1501 tattgccggg tatcggttgc tgcctgtacg gcgctgcctg cacctatgat aattcgcctg
     1561 acgaagattt tattatcgat accctacccg gccacgataa tacactgctc attaccggcc
     1621 tgagtgggca cggttttaaa tttgcgtcag ttttagggga aatagctgcc gattttgcgc
     1681 aagacaaaaa aagcgatttt gatttgacgc cattcaggct ttcccgcttc caataatcat
     1741 cattacggcc tccagaatga ggccgttttt acaggagtat ttaatgcgtc ggctgttgca
     1801 ctatctcatc aataatattc gcgagcatct gatgctttat ctttttctct ggggattgtt
     1861 ggcaattatg gatctgatct atgtgtttta tttttagttt tccgcactga cataaactga
     1921 caaatattta atcccccttt catttagcgt gaaaaattga atgagattat tcctgcttct
     1981 acagcactca ccagacatct ctgatg
//
LOCUS       DAPHDW010000278         1918 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.285, whole genome shotgun sequence.
ACCESSION   DAPHDW010000278 DAPHDW010000000
VERSION     DAPHDW010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1918
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.285"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(76..1488)
                     /gene="pykF"
                     /locus_tag="RH057_004499"
     CDS             complement(76..1488)
                     /gene="pykF"
                     /locus_tag="RH057_004499"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HDV3130731.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEQGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT          487 a          445 c          468 g          518 t
ORIGIN      
        1 cttctggtta tcgattaaat aaaaaaagcg cccatcaggg cgcttcgata tacaaattaa
       61 ttcacaaaag caatattaca ggacgtgaac agatgcggtg ttagtagtgc cgctcggtac
      121 cagtgcacca gaaaccataa ctacaacgtc acctttgtgt gccagaccgc tctgcagagc
      181 tagttcttta cccagacggt agaaatcatc agtagaagtg atctctttaa ccagctgcgg
      241 cacaacgcct ttgctcagta ccaactgatg agccgttttt tcgttggtgg tcagtgccag
      301 gatggtggca tccgggaagt atttacgtac tgcgcgagca gatttaccgc cctgagtagc
      361 aaccacgatc agcggagcat ccagtttttc agcagtttca acggcaccac ggcataccgc
      421 ttcggtaatg cgcagtttac ggttgtcatt gttgaactcg agacggctgt tcatcacgcg
      481 gtcggtacgt tcgcaaatgg tcgccatgat agaaaccgct tccagcgggt atttaccttt
      541 tgcggattca ccagacagca tcactgcatc agtaccgtcg aggatggcgt ttgcaacgtc
      601 accggcttct gcgcgagtcg ggcgtgggtt tttgatcatg gaatccagca tctgggtcgc
      661 agtgataacg actttacgtg cacggataca tttttcgatc atcatcttct gggcgaagat
      721 aacttcttct accgggattt ctacacccag gtcgccacgc gcaaccatga tgccgtcaga
      781 ggcttcgagg atttcgtcga agttgttgag gccttcctgg ttttcgattt tggagatgat
      841 gtggatgttt tcgccgccgt gcgctttcag gtgctcacgg atttcgataa cgtcagaacg
      901 cttacgaata aaggaagcag caacaaagtc tacgccttgt tcgcaaccaa agatcaggtc
      961 ctgtttgtct ttttcagcca gtgctggcag agcaatggaa acgccaggca ggttcacacc
     1021 tttgttttcg cccaggtcac cgttgttcag cactttacag ataactttgt taccttcaat
     1081 ggcggtaact tccataccga tcagaccatc gtcaaccagt acggtgttgc caactgacag
     1141 gtcagtagtg aaaccttcat acgttaccgc aaccatttcg ctgttgccga taacagattt
     1201 atcagtggtg aaagtaaagg tctgaccagc tttcagagaa acgtcgttac cgccttccag
     1261 tttcatggtg cggatttccg gacctttggt atcaagcagg atagcggcgg ttttaccagt
     1321 tttgctcatc acgttgcgca gattctgaat gcgctgaccg tgttctgcat agtcaccatg
     1381 agagaagttc agacgcataa cgttcatgcc agcgtccagc attttagcta acatctcttc
     1441 agattcggtt ttcggtccga tggtgcaaac aattttggtc tttttcatga cagtcttagt
     1501 ctttaagttg agaaggatgg gagaaacttg ctttctgggc gcactgccgg aaagcgaatc
     1561 cggtattacg aaagtggtgg tgcctcgctc taaggatagg tgacatcgaa aaagcgtgca
     1621 gaggaatgtg tgcttgtgtc tcagcccaac gtgagtgcct gtttttgagt cgttgagttc
     1681 tacagtccaa agtgctgaaa ccattcaaga gtcaattggc gcgcattata cgctgaaatt
     1741 aatccaaaag gaaaacaaaa acagcgtttc aaaaaatata caacttgtta gacaataaat
     1801 tgttatcaaa gatttaacgt tccagggaaa aggttacgca aaaacttcgc tgtttgcgca
     1861 acgtctggtg ccaaagttgt cagtgtgagg ataagtgaaa gttcatccgg tgtataaa
//
LOCUS       DAPHDW010000279         1915 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.286, whole genome shotgun sequence.
ACCESSION   DAPHDW010000279 DAPHDW010000000
VERSION     DAPHDW010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.286"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..918
                     /locus_tag="RH057_004500"
     CDS             <1..918
                     /locus_tag="RH057_004500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001045641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HDV3130732.1"
                     /translation="HLTKSAEAVLGMATAPVYIFNSELQNLRFRHGDVIENTRLPGGV
                     WGRYMGSDKRISGAVGAGYTLTQNGMETGGDKVFEFGDSQLAVGAFVSYTDNSVSHNH
                     GGNSKVGSTGGGLYATWFDNDGYYVDGVIKFNRFSNKLRTWMSDGVAVKGDYTQNGFG
                     GSLEAGKTFTLNENAWVQSYVRTTAFRANAKNIRLDNGMAARIEAARSLQGEAGVNLG
                     MNLDVNGVTVKPYLTAAVSHEFSDKNKVRINNSYDFINDISGTTGKYGAGVNAQLTPN
                     AGIWAEAGYQKGNKMESPVTGTVGFRINF"
     gene            1262..1417
                     /locus_tag="RH057_004501"
                     /pseudo
     CDS             1262..1417
                     /locus_tag="RH057_004501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907654.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1454..>1915)
                     /locus_tag="RH057_004502"
     CDS             complement(1454..>1915)
                     /locus_tag="RH057_004502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130733.1"
                     /translation="LYAIEADIRGMPAEQRLAERQRKTKPLLKSLESWLREKMKTLSR
                     HSELAKAFAYALNQWSALTYYANDGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGE
                     REALLYSLIGTCKLNDVDPESYLRHVLGVIADWPVNRVSELLPWRIALPAE"
BASE COUNT          465 a          447 c          534 g          469 t
ORIGIN      
        1 cacctgacca aatcggctga agccgtgctg ggtatggcaa ccgcgccagt ttatattttt
       61 aacagtgaac tgcagaatct acgtttccgt catggtgatg tcatagagaa cacccgttta
      121 ccgggtggag tctggggacg ttatatgggg tcagataaac gcatcagtgg tgcagttggt
      181 gctggctata cgttgacaca gaacggaatg gaaacaggcg gagataaggt ttttgagttt
      241 ggcgacagcc aactggcagt gggtgccttt gtttcctaca ctgataacag tgtcagccat
      301 aatcacggag gaaacagcaa ggttggcagc acgggggggg ggctgtatgc aacatggttt
      361 gataatgacg gttattacgt ggatggggtt atcaaattca accgtttcag caataagctt
      421 cgcacctgga tgagcgacgg tgtggcagtg aaaggtgatt acacccagaa tggttttggc
      481 ggtagtctgg aggcaggtaa aactttcact ctgaatgaaa atgcctgggt acagtcttat
      541 gtacggacaa cggcattcag ggcaaatgca aaaaatatcc gtcttgataa tggtatggca
      601 gccaggatag aagcagccag gtcgcttcag ggagaggccg gagtgaatct gggcatgaat
      661 cttgatgtaa atggcgtgac tgtgaagcct tatctgacag cggcagtgag tcatgagttt
      721 tcggacaaga ataaagtcag gataaacaac agttatgact ttatcaacga tatatcaggt
      781 acgacgggga aatatggggc cggggtgaat gctcagttga cgccaaacgc cggtatatgg
      841 gcagaggctg gctatcagaa agggaataag atggagtcac cagtgacagg aactgtcggt
      901 ttccgcataa acttctgact gtagccagaa ataccagacc gggggagcgt tccgcccccg
      961 gattttagcc tgtacagaga tttgtgtaat tgcctgattt tgagtgagcg taaaccgact
     1021 gccgtatgta accattaagc cagattactg atgcagtgaa gaccttcccg catcctgact
     1081 cacacaacaa tcgactcttt gtgtcctgtc tggacctgtc ggttgccgga agcgccttca
     1141 tgcgaggcgt ctcctcaccg atgcgcgtga ctcaagaagg gcctgacggc ttgtctcgtt
     1201 actgtcctgt ccgggttatc tgtctggaga ttcaactctg tttcctcaca ggagctctgt
     1261 catggctgat aaatataccg aagccgctgt tgtgggtggc gtggatacac ataaagatct
     1321 gcacgttgct gctgtcgtag atgagaacaa taaagtcctt gggacccggt atttctccac
     1381 aacacgacaa ggttaccggc agatgctggc ctggatgtaa gcgtacagcg agagccgtat
     1441 tgacggggat gtgttattca gctggcagtg ctatgcgcca cggaagtagt tcgctgaccc
     1501 ggttgaccgg ccagtctgct atgacgccaa gcacatggcg aaggtagctt tctggatcca
     1561 cgtcattcag tttgcacgtc ccgatcaggc tgtacagtag cgcttcccgc tcaccaccat
     1621 ggtcagagcc gaagaacagg aagtttttac gacccagact gaccgcccgc agggcatttt
     1681 cagcgatgtt gttgtcgatt tccacccagc catcgttcgc atagtacgtc agtgccgacc
     1741 actggttaag tgcgtacgcg aacgccttcg ccaactctga gtgtcgcgac agggtcttca
     1801 tcttttcacg caaccagctt tccagggatt tcaacagcgg tttcgttttt cgctgacgtt
     1861 cagcaagccg ctgctctgcc ggcattcccc ttatatccgc ctctatggcg tacaa
//
LOCUS       DAPHDW010000280         1910 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.287, whole genome shotgun sequence.
ACCESSION   DAPHDW010000280 DAPHDW010000000
VERSION     DAPHDW010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.287"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1910
                     /gene="rhsB"
                     /locus_tag="RH057_004503"
     CDS             <1..>1910
                     /gene="rhsB"
                     /locus_tag="RH057_004503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhs element protein RhsB"
                     /protein_id="HDV3130734.1"
                     /translation="AEEARQQAISGGTEPSAFPDTLPGYTEYGRDNGIRLSAVWLTHD
                     PEYPENLPAAPLVRYGWTPRGELAVVYDRSGKQVRSFTYDDKYRGRMVAHRHTGRPEI
                     RYRYDSDGRVTEQLNPAGLSYTYQYEKDHITITDSLDRREVLHTQGEAGLKRVVKKEH
                     ADGSVTQSQFDAVGRLKAQTDAAGRTTEYSPDVVTGLITRITTPDGRASAFYYNHHSQ
                     LTSATGPDGLEMRRKYDEYGRLIQETAPDGDITRYRYDNPHSDLPCATDDATGSRKTM
                     TWSRYGQLLSFTDCSGYVTRYDHDRFGQVTAVHREEGLSQYRAYDSRGQLIAVKDTQG
                     HETRYEYNIAGDLTAVIAPDGSRNGTQYDAWGKAICTTQGGLTRSMEYDAAGRVIRLT
                     SENGSHTTFRYDVLDRLIQETGFDGRTQRYHHDLTGKLIRSEDEGLVTHWHYDEADRL
                     THRTVNGETAEQWQYDERGWLTDISHISEGHRVTVHYGYDEKGRLTGERQTVHHPQTE
                     ALLWQHETRHAYNAQGLANRCIPDSLPAVEWLTYGSGYLAGMKLGDTPLVEYTRDRLH
                     RETLRSFGRYELTTAYTPAGQLQSQHLNSLLSDRDYTWNDNGELIRISSPRQTRSYSY
                     STTGRLTGVHTT"
BASE COUNT          452 a          581 c          583 g          294 t
ORIGIN      
        1 ggcagaagaa gcccggcagc aggccatttc cggcgggacg gaaccgtccg cttttcctga
       61 taccctgccg ggttataccg aatatggccg ggacaacggc atccgtctgt ctgccgtgtg
      121 gctgacgcac gacccggaat acccggagaa tttacctgcc gcgccgctgg tgcgctatgg
      181 ctggacgcca cgcggcgaac tggcggtggt gtatgaccgt agtggcaaac aggtgcgcag
      241 ctttacttac gatgataaat accggggccg gatggtggcg caccgtcaca cgggccggcc
      301 ggaaatccgt taccgttacg acagcgacgg gcgggtgaca gaacagctta acccggcagg
      361 cttaagctac acgtatcagt atgagaaaga ccacatcacc atcaccgaca gcctggaccg
      421 ccgtgaagtg ctgcacacgc agggcgaagc cgggctgaag cgggtggtga aaaaggaaca
      481 cgcggacggc agcgtcacgc agagtcagtt tgatgcggtg ggcaggctta aggcacagac
      541 ggatgccgca ggccggacaa cagagtacag cccggatgtg gtgacgggcc tcatcacgcg
      601 catcaccacg ccggatggca gggcatcggc gttttactat aaccaccaca gccagttaac
      661 gtcagccacc gggccagacg ggctggaaat gcgccgcaaa tatgatgagt acggacgtct
      721 gattcaggaa accgcccctg acggcgatat cacccgctac cgttatgata atccacacag
      781 tgacctcccc tgcgcaacgg acgatgccac cggcagccgg aaaaccatga cgtggagccg
      841 ttacggtcag ttgctgagct tcactgactg ttccggttat gtaacccgct atgaccatga
      901 ccgttttggt caggtgacgg cggtgcaccg cgaggaaggg ctgagtcagt accgcgcata
      961 cgacagccgt ggacagttaa ttgccgtgaa agacacgcag ggccatgaaa cgcggtatga
     1021 atacaacatc gccggtgacc tgaccgccgt cattgccccg gacggcagca gaaacgggac
     1081 acagtacgat gcgtggggaa aggccatctg taccacgcag ggcgggctga cgcgcagtat
     1141 ggaatacgat gctgccggac gggtcatccg cctgaccagt gaaaacggca gccacaccac
     1201 cttccgttac gatgtactcg accggctgat acaggaaacc ggctttgacg gccgcacaca
     1261 gcgttatcac cacgacctga ccggcaaact tatccgcagc gaggatgagg ggctggtcac
     1321 ccactggcac tatgacgaag cagaccgcct cacgcaccgc accgtgaatg gcgaaaccgc
     1381 agagcagtgg cagtatgacg aacgcggctg gctgacagac atcagccata tcagcgaagg
     1441 gcaccgggtg acggtgcatt acgggtatga tgagaaaggc cggctgaccg gtgagcgtca
     1501 gacggtgcat cacccgcaga cggaagcact gctctggcag catgagacca gacatgcgta
     1561 caacgcgcag gggctggcga accgctgtat accggacagc ctgcccgccg tggaatggct
     1621 gacctacggc agcggttacc tggcaggcat gaaactcggc gacacaccgc tggtggagta
     1681 cacccgcgac cgcctgcacc gggaaacgct gcgcagcttc ggccgttatg aactcaccac
     1741 cgcttatacc cctgccgggc agttacagag ccagcacctg aacagcctgc tgtctgaccg
     1801 cgattacacc tggaacgaca acggcgaact catccgcatc agcagcccgc gccagacccg
     1861 gagttacagc tacagcacca ccggcaggct gaccggcgtt cacaccaccg
//
LOCUS       DAPHDW010000281         1859 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.288, whole genome shotgun sequence.
ACCESSION   DAPHDW010000281 DAPHDW010000000
VERSION     DAPHDW010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1859
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.288"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..634
                     /locus_tag="RH057_004504"
     CDS             <1..634
                     /locus_tag="RH057_004504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130735.1"
                     /translation="VLHHYRGKLTEVLKVVIKKENVFSERTGESLHINARRFRYTLGT
                     RAAREGYGEFIIAELLDHSTIKSVGVYVQNHVDNAYKIDKTMGQALTGISCVFRGEVK
                     RKEDIEYDITPDIKIKNHDGEDTGSCKQCSTCSANVPIPCYTCMHFTPWLDGPHEKVY
                     QYLIGERERIYSITNDSIVTESLDRTIIAVKEVINHCNIMRSSHKGNKSI"
     gene            631..>1859
                     /locus_tag="RH057_004505"
     CDS             631..>1859
                     /locus_tag="RH057_004505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV3130736.1"
                     /translation="MTDSNVLFFRKKIDINAELNLKEFLRFCRYDLTALGSDFNWESY
                     EWRGVAVFTKINLKRKVKTDKEQLLDEEYIDFAKAYMRYQSALTKSRWKLSASLTILK
                     VVEQTLLMVNKNANITGVSIPVLDEAIQTIKSLYKEHPAYDYGLELQKFVKFLGENGL
                     LIAGVLSWVSPIKPPEPVNRVSKKADEARLSKLPDETALNAMAEIFSLPDEALSCRDI
                     FTTSIFALLMCAPSRISEILALPCDCEITETDSNGVERYGLRFYSLKGYGADIKWIPD
                     VMVPVARRAIERLKKLSKNAREFAQWVEENPSTFFRRDSLPETGENEILKPWQICEGL
                     GYNLERKNGNVKFLSGVSFYNSQKKYTMNSLWEIILSRLPEGFPWYDKNKGIKYSNAL
                     CLLNIHQLEKRNETIIY"
BASE COUNT          628 a          316 c          415 g          500 t
ORIGIN      
        1 tgtattgcat cattatagag ggaaattaac agaggttttg aaagttgtca tcaagaagga
       61 aaatgtcttc tcagaaagga ctggggaatc cttacatata aatgccagaa ggtttcgtta
      121 tacgttagga acacgagcag caagagaagg ctatggagaa ttcattatcg cagagctact
      181 tgatcactcc acgattaaga gtgtaggagt ctatgttcag aatcatgttg ataatgctta
      241 taagattgat aaaactatgg ggcaggcatt gactggaata tcatgcgtgt tcagaggtga
      301 agtaaaaaga aaagaagata ttgaatatga cataactcca gatataaaaa ttaaaaatca
      361 tgatggcgaa gacactggca gttgtaagca atgttccaca tgttcagcta atgtccccat
      421 tccttgttat acatgtatgc attttacccc ctggctggat ggaccgcatg aaaaggttta
      481 tcagtatttg attggcgaaa gagaacgaat atacagcata actaacgaca gcattgtaac
      541 ggaatcgctc gacagaacga tcattgctgt aaaggaagta attaaccact gcaacataat
      601 gcgaagctca cacaagggga ataaatccat atgaccgata gtaatgtatt attcttcagg
      661 aaaaagattg atataaatgc agagttaaat ctgaaagagt ttctccgatt ttgcagatac
      721 gatcttaccg ctttagggag tgattttaac tgggagtctt acgagtggag aggtgtcgct
      781 gtttttacca aaataaattt aaaacgtaag gtaaaaacag ataaagaaca attgctcgat
      841 gaggagtata tcgattttgc taaagcttat atgagatatc agtcggcttt aaccaaaagc
      901 agatggaagc tgtcagcttc attgaccata ttaaaagttg tcgaacaaac attactgatg
      961 gtcaataaaa atgcaaatat caccggggtc agcatacctg ttcttgatga agccatccaa
     1021 accatcaaga gtctttacaa agaacatccg gcctatgatt atggactgga acttcagaaa
     1081 tttgtgaagt tccttggtga aaatggtcta ttgatcgcgg gtgtcctttc atgggtcagc
     1141 ccaataaaac caccggaacc tgttaaccgt gtgtctaaaa aagcagatga agcaaggtta
     1201 agtaaacttc cggatgaaac ggctttgaat gctatggcag agatcttctc actgcctgat
     1261 gaggcattga gttgccggga tatttttact acttcaattt ttgcgctcct gatgtgtgct
     1321 cccagtcgga tatcggagat tttggcactt ccctgtgatt gcgagattac ggaaacagac
     1381 tctaacgggg tggaaaggta tgggctaagg ttttactccc tcaagggtta tggtgcggac
     1441 attaaatgga ttccagacgt tatggttccc gttgccagga gggcaataga aaggttaaag
     1501 aaactttcaa aaaatgcccg tgaattcgca cagtgggtgg aggagaatcc atctacattc
     1561 ttcagacggg atagccttcc tgaaacagga gaaaacgaaa tattaaaacc ctggcagata
     1621 tgtgaaggat taggatataa ccttgaaaga aaaaatggga acgtaaagtt tttatctgga
     1681 gtgagttttt ataatagcca gaaaaaatac accatgaact cattatggga aattatcctc
     1741 tccagattac cagaaggttt tccatggtat gataaaaata aaggcataaa atacagcaat
     1801 gcactttgcc tgttgaacat ccatcaactg gagaaaagaa atgaaactat catttacga
//
LOCUS       DAPHDW010000282         1855 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.289, whole genome shotgun sequence.
ACCESSION   DAPHDW010000282 DAPHDW010000000
VERSION     DAPHDW010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.289"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            170..661
                     /locus_tag="RH057_004506"
     CDS             170..661
                     /locus_tag="RH057_004506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014838325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HDV3130737.1"
                     /translation="MDTDKLVTLFRYKRWIDIETLQAIKRIDTSVNAEKHHLTLRLMN
                     HIHVVDSIFRANVTGQKHSYTALNTPETPSADELLITMTECTDWYIQHVNSMSSAELA
                     ETIKFQFVDGGHGEMKAFDMLNHVLFHGTYHRGAVGWLISESGVVPPKDVLTVFLRDH
                     NHE"
     gene            867..1535
                     /locus_tag="RH057_004507"
     CDS             867..1535
                     /locus_tag="RH057_004507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001192368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HDV3130738.1"
                     /translation="MQSVYAIVTVNLYSYHGHHKMKKVILFLCQGLEEYEASVFTDTI
                     GWTTTYGLEPIELVTVGLRQKVKCAWNFTIEPEYQLHEIDDDGFDAIAIPGGMSRAGF
                     YEDAFDERLLRLIQKFNAKNKIIASVCVGALAIAKSGALQGRTGTTYHLSSKRQAQLL
                     EMGVHVVQHPIVVDGNIITSQSPATAINVAFTLIEKLTSRANVERIKEGMGFAPLSID
                     PSGM"
BASE COUNT          553 a          380 c          401 g          521 t
ORIGIN      
        1 gccctcaagg tctgcatttt ctgagagctt gaatcccact caacgtccgc tcttcgctca
       61 cagcggacct gagtacctag tttctgtccg ttcagtgcca aaagcggaag taagtagaga
      121 accttttatg ctgctgatat gataataaaa tgtataaaag aggtggtgaa tggatactga
      181 caagttagtt acgctgttta gatacaagcg ctggatagat attgaaacac tacaagcgat
      241 caaaagaatc gatacgtctg taaatgccga aaagcaccac ctgacgctca gattaatgaa
      301 tcacatacat gtggttgact cgatcttcag agctaacgtt acaggtcaga agcattccta
      361 cactgcactg aacacgccag agaccccatc tgctgatgag cttctgataa cgatgactga
      421 atgtacagat tggtatattc aacatgtcaa ttccatgtct tccgctgagc ttgctgaaac
      481 aatcaaattc cagtttgttg atggcgggca tggtgaaatg aaggcgttcg atatgctcaa
      541 tcatgtgctt tttcacggga catatcatcg tggcgcagta ggttggctaa tttcagaaag
      601 tggtgtagtc ccgcccaagg acgtactgac cgtttttctt agagatcata accacgaata
      661 aacgactggc aggggcatgc aaatttataa ctccattgaa cccttacaca aaaaagtaag
      721 tgcataaatg aatgtcatgg agtgtccgct tctcgctcaa agtagtcctt cagtgtcacg
      781 tgcttgtctg ctgtgtgcca aaagcggaaa ttcactccat agtctctcaa gtggtagatt
      841 aaacccgtac cgacaacagg ttaaaaatgc agagtgttta tgctatcgtt actgtgaatt
      901 tatatagtta ccatggacat cataagatga agaaagttat cttattcctt tgccaaggat
      961 tagaagaata tgaggctagt gtattcacag atactattgg ctggacaaca acatacggat
     1021 tagaacccat agaattagtg acagttggtc tacgtcaaaa agttaaatgc gcctggaatt
     1081 tcactatcga accagaatat caactacatg aaattgatga tgatggcttt gacgccatag
     1141 ccattcctgg ggggatgagt cgggccggtt tttatgaaga tgcatttgat gagcgccttc
     1201 taaggcttat tcaaaaattt aacgcaaaaa acaagattat tgcttctgtt tgcgtaggtg
     1261 ccttagcaat tgccaagagt ggcgctttac aaggaagaac gggcacaacg tatcatctga
     1321 gcagtaaacg tcaagctcag ttgctagaaa tgggagtcca tgttgttcag caccctatag
     1381 tggtggacgg aaatattatt acgtctcaaa gtccggcaac agctattaat gtagcattta
     1441 ctttaataga aaagctgaca tctagagcta atgttgaacg aataaaagaa ggtatgggat
     1501 ttgcccccct ctctatcgat ccatccggaa tgtagtcctc ttctcgctca ttacagacct
     1561 tctgttccaa gaggttttcc acttagtgcc aagagcggaa gttcctaacg tcatgctgca
     1621 ttcgtttaag gggagcagat cagcacttct caaacatggg cagtctctag ggcatttgaa
     1681 gcttttgata agtactatag ttgccgttag ttattcttaa tctgctgtag aacttgtcct
     1741 tccctaagtt aactactcga caaagttgta cctcaactga ataaagtaca gtaaaaaggg
     1801 agattttttc ttttcctcga acaagtaatt ttgaaacatc atactgcact gcatg
//
LOCUS       DAPHDW010000283         1854 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.290, whole genome shotgun sequence.
ACCESSION   DAPHDW010000283 DAPHDW010000000
VERSION     DAPHDW010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.290"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(70..393)
                     /locus_tag="RH057_004508"
     CDS             complement(70..393)
                     /locus_tag="RH057_004508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HDV3130739.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAVPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            657..743
                     /gene="azuC"
                     /locus_tag="RH057_004509"
     CDS             657..743
                     /gene="azuC"
                     /locus_tag="RH057_004509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HDV3130740.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            858..1109
                     /locus_tag="RH057_004510"
     CDS             858..1109
                     /locus_tag="RH057_004510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HDV3130741.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTNHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(1188..1691)
                     /locus_tag="RH057_004511"
     CDS             complement(1188..1691)
                     /locus_tag="RH057_004511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HDV3130742.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
BASE COUNT          528 a          408 c          440 g          478 t
ORIGIN      
        1 acgaatggga ataaaacgca ttaccgctat ttctttaatc agcgaagatc ccctcgctga
       61 ttaaaatatt tagagaaaga cagggaaggc ggaatgatgg cactgatatg acaaagtgaa
      121 ttccgtattc aaacataagg aacccgcaaa aaggctacag ttgcctactg gctgaccaaa
      181 ggggaccgcg tgggtatagc cttcctgctg gcatgcgcga tcggcgatcc cgcgactgac
      241 ataacgatcg gtatgggcat gctgcaaaaa agcctggtca taaactaacc gtacaatacc
      301 tgttgccgcg tcaacttcgc tcacatgggc ctggcgagta accgtacacc ccgccagcgt
      361 tattaatgaa agagtcagaa tcagaagtct catggtccag ccagaagagt aagagaaagc
      421 actattttac gcgttacagt gctgcgacaa tggccggaaa agacgcctgg caggcgctat
      481 ttcttgccat ctgtaggtgt cagaaatagg gggggctgtt ttttgattat catttttata
      541 acattaaagg ggtgtttatg tgatttgtat cacataaaga ttgatttttg tgatggttgt
      601 catattataa gcgcaaagac aataacacct gtataacaaa tggtcggagt gccgcgatga
      661 aactgcgcaa aatcctgaaa agtatgttca ataactattg caagacgttc aaagacgtac
      721 cgccaggcaa tatgttccga taacaaaaaa cctgctccgg caggtttttt tgtgtcctga
      781 tgacggtgga tgtgactatc gctgaagatg tgaagccgct actataacgg ctttcaaaat
      841 gctatcagga gtttacgatg tcccagccgc tgaatgccga tcaggaactg gtttctgatg
      901 tggttgcttg ccagttggtt atcaaacaaa ttctggatgt tctcgatgta atcgcgcccg
      961 ttgaagttcg tgaaaaaatg tccagtcagc tgaagaatat cgactttact aaccatcccg
     1021 ccgccgcaga cccggtcacc atgcgggcaa tccaaaaagc catcgcgctt attgaactca
     1081 agtttacccc acagggtgaa tcccattaaa aaaaacgctg cttcaaacag atgaggcagc
     1141 ggtttctcaa aaccttcatc ggcgcaatgc attagcgccg atgatgatca atgcagctgg
     1201 tgtgacacga cattcagaac atgttggtcg gtctgcacag ggcacatacc cgcaagtttc
     1261 gcactgcgca cttcatcaag aatggtttgc agcaacagac catcatgctg ctgttctttt
     1321 tccagatcgc gcaggaaatt gacggttgaa tcatcattca gttctttcgc ttcatcggct
     1381 aactgtgcca acgtactaga acgttgctcg tattcttcca tcgttttttg gaacagttct
     1441 tccagagagt tcagtttttc accgggaaca tcaatggctt taacgatggg ggtagcgccg
     1501 acactcttca taaagttaaa catgcgcatc atttgggtca cattactctg tgcctgggcg
     1561 cgaaggaaag tggcggtgcc gttcagactc tgttcagaac accagttact caggtgaagg
     1621 tagagattgg atgcgtaaaa ctcgcggttc atttgagagt tgagtttgag aagcattcca
     1681 gcggttgcca tacctaatat ccttatatcc agaagtggag aggtgcaaga taaatcaaaa
     1741 caccataatt actgtttgca gaataattcc tttctgccgg tccgaaatgt gatcgtggag
     1801 tcaattctga cgatgatctg aaataagaat aacgtctgaa cgggtataag aaaa
//
LOCUS       DAPHDW010000284         1853 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.291, whole genome shotgun sequence.
ACCESSION   DAPHDW010000284 DAPHDW010000000
VERSION     DAPHDW010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1853)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1853)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1853
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.291"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..778
                     /locus_tag="RH057_004512"
     CDS             <1..778
                     /locus_tag="RH057_004512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HDV3130743.1"
                     /translation="CGSGTVIKNNGTTDAPKWHKWNKVILNNHLGTFPNLQSLNQVRE
                     GMTKDQLYYLIGRPQYDDGWRPREWNYLFHFYTPGKGVNNVTTCQYKILFDSKMIARS
                     FYWHPVIPEDSVCPPQKSSASQKFTQRYTLNADALFPFDKSDASDINQQGIVSLDNLA
                     MKLNSFEQLNSIRVIGYTDRLGSPDYNSILSKKRADTIRQYLIAKGIPAGKIIAIGMG
                     KNQPVKECDYENNRTALINCLQPNRRVEVEVDGIIRISDE"
     gene            775..1774
                     /locus_tag="RH057_004513"
                     /pseudo
     CDS             775..1774
                     /locus_tag="RH057_004513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          576 a          342 c          448 g          487 t
ORIGIN      
        1 ttgtggttca gggactgtta ttaagaataa tggtacaacc gatgctccca aatggcataa
       61 atggaataaa gtgattttga acaatcacct tggaactttt cctaacctgc aaagcctaaa
      121 tcaggtacgt gagggtatga ccaaagacca gttatattac ttgattggtc gaccgcaata
      181 cgatgatgga tggcgaccac gggaatggaa ctatctgttc catttttata ctcccggtaa
      241 aggtgttaat aatgtcacaa catgtcaata taaaatctta tttgacagta agatgatagc
      301 ccgtagtttt tactggcatc cggtaattcc ggaagatagt gtgtgtccac ctcagaaatc
      361 atcggcatca caaaaattta cacagcgata tacgctgaat gccgatgcac ttttcccatt
      421 tgataaaagt gatgcgtctg atatcaatca acagggaatc gttagcctgg ataatctggc
      481 tatgaaactg aattcttttg agcaactcaa tagcatcagg gttattggct atacggacag
      541 gttggggagt cctgattata atagtatttt atcgaagaaa cgtgctgata caattcgtca
      601 gtacctgatt gctaaaggaa tacctgcagg caagattatt gctattggta tggggaaaaa
      661 ccagcctgta aaagaatgcg attatgaaaa taacaggact gcacttatca actgcttaca
      721 accaaaccgg cgtgtggagg tagaggtgga tggtatcatt agaataagtg atgaataagt
      781 agaactcctc aggaagaaat cgtcattatt cagcagacaa tagagccagg agtgactgtg
      841 tcccttatgg cgcgtttgca tggtattcag cccagccagc ctgttgttta aatgaaaaac
      901 aatataagga aggaggtctc cctgctgttg ctgccggaga acaggttgtt cctgctgccg
      961 ctataaaata aataaaagag ttacagcact tgctgggtaa gacaaccatg gaggtttata
     1021 tcatcaacga agctgtggag tataattact cgtgaaaatc gatagagcat gcgccttggt
     1081 gcccaacgga taaggaacaa ctactgtaag ctgtattctg ggcgtgtcgc gtgctcaact
     1141 gacacaacgg atgaaaacat ctggtaataa gctggataaa cgtcgtcgca gacgcgatga
     1201 tgctgcggat gcggaagtgt tagcacggat atgccgacat atggttatcg tggtgtctgt
     1261 gccattctca gataacagtc ccgtaacgaa ggactgctgt ttgtgaatgc aaagcgggtt
     1321 tatcggataa tgaccgaaaa taacctgttg ttacatgacg aaccttcatt gccacagcgg
     1381 aaacatataa aggtcgaata tcggtgaagg aaagcgacca gctctggtgt tacgatggct
     1441 ttgagttagg ttgcgatgat ggcgaaaaac tgcgtgtcac gttcgcactt aactgttgcg
     1501 atcgtgaagc cataaactgg gccgcaagta cgtgaggtta tgacaaaaaa cagtgcagga
     1561 tgtcatgtta ggagcgatag ataacggaat ggcagaaagg tttttgaaaa cgatgaagga
     1621 agactatatc gttttcatgc acaaaccaaa tgtaagacca gctttgcata accttgtagt
     1681 ggcgatcgag cattacaatg aaaacaatcc gcatagtgcg ttaggttatc gctcgccacg
     1741 gaaatatcga cgtcagcggg taacgttaac ttaagataca acaactgtct gaatatagtg
     1801 gactaaggac atcctgttgt caggatatgg gtttttgtta attattaaaa ata
//
LOCUS       DAPHDW010000285         1836 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.292, whole genome shotgun sequence.
ACCESSION   DAPHDW010000285 DAPHDW010000000
VERSION     DAPHDW010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.292"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            47..829
                     /locus_tag="RH057_004514"
     CDS             47..829
                     /locus_tag="RH057_004514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HDV3130744.1"
                     /translation="METKQKERIRRLIEILKKTDRIHLKDAARMLEVSVMTIRRDLHQ
                     EDEPLPLTLLGGYIVMVHKPAPSMPVIQDVPRNHRDDLPIAILAAGMVNENDLIFFDN
                     GQEIPLVISMIPDAITFTGICYSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNS
                     SPLDSLNPRKIFISASGVHDHFGVSWFNPEDLATKRKAMARGLRKILLARHALFDEVA
                     SASLAPLSAFDVLISERPLPADYVTHCRNASVKIITPDSEDE"
     gene            complement(831..1057)
                     /locus_tag="RH057_004515"
                     /pseudo
     CDS             complement(831..1057)
                     /locus_tag="RH057_004515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005229544.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
BASE COUNT          501 a          466 c          396 g          473 t
ORIGIN      
        1 actaatgcca taatgttatt tttataacat tttacggaga gagttgatgg aaacgaagca
       61 aaaagagcgt atccgacgtt tgattgaaat acttaagaaa accgacagaa tccatttgaa
      121 agacgcggca cgaatgctgg aagtttctgt aatgactatt cgtcgcgatc tccatcagga
      181 agatgaacct ctgccactga ccctactggg tggctatatt gtaatggtgc ataaacccgc
      241 accatccatg ccagtaatcc aggacgttcc gagaaatcat cgtgatgact tacctattgc
      301 aattctggcc gccggaatgg ttaatgaaaa tgatctgatc ttctttgata atggccagga
      361 gataccgctc gttataagca tgatcccgga tgcaatcacc ttcactggca tctgttactc
      421 acatcgtgtc tttgttgcgt tgaatgaaaa acctaatgtg acagcaatac tttgtggtgg
      481 tacgtatcgt gccagaagtg atgcttttta cgatgccagt aactcttcgc cattagactc
      541 tctcaatccg cgaaaaatat ttatttccgc cagcggtgta catgatcact ttggcgtcag
      601 ctggtttaat cccgaagatc ttgccactaa gcgtaaagcg atggcccgtg gactaaggaa
      661 aattttgctc gcccgccacg ccttgttcga tgaagtagcc tctgccagcc tcgcaccgct
      721 ctctgcattt gatgttctga ttagcgagcg tccgttaccg gcagattatg ttacgcactg
      781 ccggaatgct tctgtaaaga tcattacacc tgattcagaa gacgaatgac ttactgaaaa
      841 aacaccgtac tcttgttaaa catcgtcgga ttggactgat tacgttgcac tttcatcaca
      901 tattccagtt tatcaatttg gcttatcatc tactccagac gctggtcatc attgaccaat
      961 agtctgatat ggcttttgtc gctgtcctga atcggcaggc agaaaatgcc ttcaacgtta
     1021 aaagcacgac gggaaaaagc ccacaaaggt tagtcattac gcccggataa taaacaggct
     1081 cctccctgtt atggaagccg ttgtattgtc cataatgtct gggtttttaa tgcacactgg
     1141 ataacaaccg gacgaatctt caaatattac tggcgctgac agccggtttg aattcatctc
     1201 acaaccctgc atagagcgaa tctcctgcct gcacgtcact ccactccatg gtatcaactt
     1261 cacactcttt atctgcggct agtttcagtc accagataag catcgactat gaaaggtgag
     1321 ccatgacaac ataacgttgg taacgctctg acgccttaac ggaagatgcc tgccaccaca
     1381 gggaatgtaa acgactgaag tgtggccttt aatgccgtga acggctcatg gtctcctggc
     1441 acggttgccg ccccaacctg taacaacatt ccacagtaca atgtctgtca gagccggagc
     1501 ctcccatgcc tgttgaataa aaccccggat gcttcccccg attctgcaag taatacttcg
     1561 gttatttaca ctattttatc cctgttgaat ttggttaagc cggtgcagac gcgccgggaa
     1621 acgcacgcct gaaccggcac attaaaatac aatacaaccc cctgttactg gagatacgaa
     1681 tggaatgacg aggcagcctg tcagtcacag gagggctgtt ggctcaaaat caacggaaag
     1741 gccaccaaca ccggaattaa gctccacagt ccccgcactg gcaatccatt cccgggaacg
     1801 gcatcaccgc aaactgcgcc agaggcaacc attaca
//
LOCUS       DAPHDW010000286         1829 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.293, whole genome shotgun sequence.
ACCESSION   DAPHDW010000286 DAPHDW010000000
VERSION     DAPHDW010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1829
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.293"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1829
                     /locus_tag="RH057_004516"
                     /pseudo
     CDS             1..1829
                     /locus_tag="RH057_004516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family protein"
BASE COUNT          638 a          362 c          345 g          484 t
ORIGIN      
        1 aaatttccct ttctccagat acaacttatc gtgagttgaa taaagcagca cagcgtagca
       61 ttacagcgcc agacaggata ggagaaggaa aatcatgggt ttatcaaaga gatacaatga
      121 ttgaaagagg tgataacagc ggtgttcatc agtatggtcc tgctgaacat ttcacccaca
      181 ttatatctga caaaccgtcc ccaaaagatg agtatattgc atatgctatt aacattcctg
      241 actatgagct ggcagccgat gtatataata ttaacgtgac gtcaccttcc gaacagcaag
      301 aaacatttaa aatactaatc aatccagaac atctacggca aacacttgag cgtaaatctc
      361 ttactgctgt tcagaaatca caatgtgaaa tcataacccc caaaaaacct ggcgaagcga
      421 ttcttcatgc ttttaatgcc acctaccagc aaatcaggga aaatatgtct gagtttgcac
      481 gttgccatta tgggtatata caaatccctc cagtgacaac tttccgcgcc gacggaccag
      541 aaactcccga agaagaaaag ggttactggt ttcatgctta tcaacccgaa gatctttgta
      601 ccatccataa tccaatggga gatttgcagg attttatcgc attggttaaa gatgctaaaa
      661 aatttggtat cgatatcatt cctgattata cctttaactt tatgggaatt gggggtagtg
      721 gtaaaaatga cctggattat ccctctgctg atatacgagc gaagatcagt aaagatatag
      781 aaggtggtat ccctggctat tggcaaggtc aggttttgat tccattcatt aaagatccag
      841 taacaaaaga acgtaaacaa atccatccag aagatataca tctcactgca aaagacttcg
      901 aagcaagtaa agataacata tctaaggatg aatgggaaaa cctccatgca ttaaaagaaa
      961 agcgtttaaa tggaatgcct aaaacaacac ccaaaagtga ccaggttatt atgttgcaaa
     1021 atcaatacgt tcgtgaaatg cgaaaatatg gcgtacgagg tttacgttac gatgcagcaa
     1081 aacactcaaa acatgaacaa atagaaagat caataactcc accgcttaaa aattataatg
     1141 agcggttaca caatactaat ttatttaacc caaaatatca aaaaaaagcc gttatgaatt
     1201 acatggaata cctggtaact tgtcagttgg atgaacaaca aatgtcatcg ctgctttatg
     1261 aaagagatga tttaagcgcc attgattttt cattgctcat gaaaacgata aaagcctttt
     1321 catttgatgg cgatctccaa acccttgcat caaaaccggg ttccacaatc tcaagtatcc
     1381 catcagaaag acggatattg attaatcatt aaccacgatt ttccaaacaa tggcaatctt
     1441 ttcaatgact ttctatttaa ccatcaacaa gatgaacaat tagcaatggc atatatagcc
     1501 gctctgccgt tcagcaggcc tttagtttac tgggatggcc aagtattaaa atcaacgact
     1561 gaaattaaaa attatgatgg gtccacgcgt gtcggcggtg aggcgtagct taataaaggt
     1621 tgctctacct atcagcagct ctacaatgaa ttccacgcat tatatataga taaagcagga
     1681 atatggagcg catttgaggg tgtatctgca actaaaaacg ttctggcctt tagtcgtggg
     1741 gattctgtga acattaatca ctctcctcat gatggactag ttataataaa taaaggaaac
     1801 gaagaagttg aaggtacctg gcctaacaa
//
LOCUS       DAPHDW010000287         1819 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.294, whole genome shotgun sequence.
ACCESSION   DAPHDW010000287 DAPHDW010000000
VERSION     DAPHDW010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.294"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            261..833
                     /locus_tag="RH057_004517"
     CDS             261..833
                     /locus_tag="RH057_004517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001315613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HDV3130745.1"
                     /translation="MASATSENTSSTKNEAQTVLSERSLKAEQRGMEIMDDMQYARLA
                     LFSGDTGAADKLINRAEDLLKSDDKTWETVVRKDKKTPQEGDVYIIINSSLMLSEDFT
                     VTPEKQKAIDEANKKLDKGDKKGAIDVLHLAGIDISETQWLMPLKQTQKKVNSAQKLM
                     SEGKYYEANLALKGAEDGVITDTVSLNEGD"
     gene            989..1723
                     /locus_tag="RH057_004518"
     CDS             989..1723
                     /locus_tag="RH057_004518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016232889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HDV3130746.1"
                     /translation="MFPYCFAIIVRPLCASFYNEELDFARNEILFLKRNDIQYITDAC
                     EGCVRFIDIPCHIISDYLYIRLRDADALCITDRKNSFFRKKAYGTFLNSLMSLAEQQK
                     NTNLYESVLFVLLDYLNDKNQLLPVLSGGLNNYSLRVEAIVAAEPAKKWYLSDVASAL
                     CISASQLKRKLHSEGAGFSRIITEVRMKKAITLMKCGNANIFIVARECGYSSLSYFIL
                     SFRKYYSVTPCQWLKQYGIKDTTGDG"
BASE COUNT          569 a          319 c          414 g          517 t
ORIGIN      
        1 attttttgtg tgggggtaag tggtcagatt tcttatatca gttgcagaac ataaaatatg
       61 catgccggat aggggagtat aaatgtccgg gagggggaca tccttgtccg gcttcctgag
      121 tatatgacta atcgtcaggg ggaaaatcat attatgttta ttatcaggta tgattctttc
      181 tgcctgatgt cggagtaaaa aatatgaaac atatctttaa tgctgtagca atcgctctgt
      241 ccctgacctc tgctgtcgca atggcctcag caacttcaga aaacacttca tccacaaaaa
      301 atgaggctca gacagtttta tcagaaagga gtctgaaggc agaacagcgt ggtatggaaa
      361 ttatggatga catgcagtat gcacgtcttg ccttattcag tggtgacaca ggagcagcag
      421 ataagctgat aaaccgggct gaagatttgc ttaaatctga tgataaaaca tgggaaacag
      481 ttgtccgcaa ggataaaaaa acaccacagg aaggcgatgt ctacattatt attaacagca
      541 gcttaatgtt gtctgaagac tttactgtca ccccggaaaa acagaaagct attgatgagg
      601 ccaataaaaa actggataaa ggtgacaaaa aaggagcaat tgacgtactg catctcgccg
      661 gaattgatat ttcagaaacg cagtggctga tgccattaaa acagacgcag aaaaaggtta
      721 acagtgcgca aaaattaatg agcgaaggga aatactacga agctaatctt gcactgaaag
      781 gtgcagaaga tggtgtgatt acggatactg tcagcctgaa tgaaggtgac tgataacatt
      841 tatatgcgcg tataaccacg gtgaccggac tgtattttgt ccggttactt taataataaa
      901 accgaacttc ttaatataat gtcaggaagt aagtctgtca gctataataa gaaaaatacc
      961 ggatatttct aaataacacc tgtactgtat gtttccgtat tgttttgcca ttatagtgag
     1021 gccactctgt gcttcatttt acaatgaaga gcttgatttt gcccggaatg aaattctctt
     1081 tcttaaaaga aatgacattc agtatattac ggatgcctgt gaaggatgtg tgaggtttat
     1141 tgatataccc tgtcatatca tcagcgacta tctgtatatc agactgaggg atgccgatgc
     1201 gttatgcatc actgacagga aaaattcttt tttcagaaag aaagcgtatg gtacctttct
     1261 gaactcgctg atgtcacttg cggaacaaca gaaaaatact aacctttatg agtcagtatt
     1321 atttgtgctg ctggattatc ttaatgataa aaaccagttg ctgcctgtat tgtccggtgg
     1381 gctgaataac tactcgttac gtgttgaagc tattgttgcc gcagagccgg caaagaaatg
     1441 gtatctgtca gatgttgcat cagcgttatg tataagtgcc agtcagctta aaagaaaact
     1501 gcacagtgaa ggcgcaggat tcagccggat aatcacagaa gtacgtatga aaaaggcaat
     1561 aactctgatg aagtgcggga atgctaacat ttttattgtc gccagagagt gcggatatag
     1621 cagtctgtcg tattttattt taagtttcag aaaatactac tcagtcactc cctgtcagtg
     1681 gttaaaacag tatggtatta aggacacaac tggtgacggg taaaccctga ttcacataaa
     1741 ccggttttct ggcgtataca tgctgtacag tcatccaggc cttataaggg gtatattgat
     1801 tgttttgtga tatgtttca
//
LOCUS       DAPHDW010000288         1819 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.295, whole genome shotgun sequence.
ACCESSION   DAPHDW010000288 DAPHDW010000000
VERSION     DAPHDW010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1819)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1819)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1819
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.295"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1324
                     /locus_tag="RH057_004519"
     CDS             <1..1324
                     /locus_tag="RH057_004519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HDV3130747.1"
                     /translation="LSDNYNAITLGNGWNTPLGAISFYAARSSSKLNNDTRHEGTSYQ
                     VAYNKYLVQTATRFSVAAWRYASQDYRTFSDHLYENDKHYHQSDYDDFYDIGRKNSLS
                     ANIMQPLSNNLGNVSLSALWRNYWGRSGNAKDYQFSYSNNWQHISYTFSASQSYDENN
                     KEEERFNLFISIPFYWGDDIAKTRHQINLSNSTSFSKDGYSSNNTGITGIAGEHDQLN
                     YGIYVNQQQQNNDTSLGTNLSWRTPIAIIDGSYSHSKNAWQSGGSISSGLVVWPGGIN
                     ITNQLSDTFAILDAPGLEGAHINGQKYNRTNSKGQVVYDLIIPHRENHLVLDIANSES
                     ETELQGNRQIIAPYRGAVSYVQFTTDQRKPWYIQALRPDGSPLTFGYDVLDLQENNIG
                     VVGQGSRLFIRVDEIPTGIKVALNDEQNLFCTITFQHVIDENKTYICQ"
     gene            1337..1819
                     /locus_tag="RH057_004520"
                     /pseudo
     CDS             1337..1819
                     /locus_tag="RH057_004520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
BASE COUNT          579 a          353 c          351 g          536 t
ORIGIN      
        1 tttatccgat aactataatg ctataactct gggaaatggt tggaatacac cgctgggtgc
       61 catctctttt tacgctgccc gatcgagtag taagttaaat aatgatacca gacacgaagg
      121 tacgagctat caggtcgcct ataataaata tctagtacaa acagccactc gatttagcgt
      181 ggccgcctgg cgttatgctt cgcaggatta caggacattc agcgaccatc tttacgaaaa
      241 tgataaacac tatcatcaga gtgattatga tgatttttat gatattggca gaaaaaatag
      301 cctttctgct aatattatgc aacctttatc caataatctg ggaaatgtat cattaagtgc
      361 gctttggcgg aattactggg ggcgaagtgg aaatgctaaa gattaccaat tcagttactc
      421 caataactgg caacacatta gttatacttt ctctgccagc caatcttatg atgaaaataa
      481 taaagaagag gagcgtttta atctgtttat ctccattcct ttctactggg gggatgatat
      541 tgccaaaaca cgtcaccaaa ttaacttatc gaattcgacc tcattttcca aagatggcta
      601 ttcctccaac aatactggaa ttactggcat agccggtgaa catgatcagt taaattatgg
      661 tatatatgtt aatcagcaac aacaaaataa tgatacctcg cttggtacga atttaagctg
      721 gagaactccc atcgccataa tagatggcag ctatagtcat tctaaaaacg cctggcaaag
      781 tggtggaagt attagtagtg gattagttgt ctggcccggt ggtattaata tcactaacca
      841 gttatccgat acatttgcaa ttctggatgc gcctgggtta gaaggtgcac atattaatgg
      901 acaaaaatac aatcgaacaa acagcaaagg ccaggttgtt tacgacctga taatacctca
      961 tcgtgaaaac catctggtac ttgatatagc aaacagtgaa agtgaaacag aattgcaggg
     1021 caatcgtcaa attattgcac cttaccgtgg agcagtttct tatgtgcagt ttacaactga
     1081 ccaacgtaag ccctggtata tacaggcact gcgtcccgat ggttcgccat taacctttgg
     1141 ctatgacgta ctggatctcc aggaaaataa tattggagtc gttggccagg gtagtcgcct
     1201 ttttattcgc gtagatgaaa ttcccactgg aataaaagtt gctctcaatg atgaacagaa
     1261 cttattctgt actattactt ttcaacacgt tatcgatgaa aacaaaacat atatatgcca
     1321 gtaaaaggag tttactatga agtggctttt attaatcacc ttgagtctat attcttttat
     1381 tgtgcaaagt gctccctgtg cattgaccaa tgtcggtgaa caacgtggaa catatatact
     1441 caagccactg agtatgaaag gtaatctaac cgccgaaaaa ttgaaaacac cggaaatatc
     1501 attgagtgta cagacttgca agagacaacc aacgccatta aagttcattt tttaacacag
     1561 aacgctttaa ggtttaaagt cggaagtcgt cattatatta ttaatttccc ctttgaatct
     1621 gacggtaaaa aaatagagct tccagggaag tcatcttata atttagaaga aatgctatct
     1681 gcgataaatt tcaccattcc atattctatc gcatctgtat cagatagttc taacaccgca
     1741 gacatttttc ctggaactcc ttttcctttg ttgttcgaaa ttgaagtgag ttcatgtaca
     1801 gggaataata ctaattgta
//
LOCUS       DAPHDW010000289         1772 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.296, whole genome shotgun sequence.
ACCESSION   DAPHDW010000289 DAPHDW010000000
VERSION     DAPHDW010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.296"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            31..1437
                     /gene="gndA"
                     /locus_tag="RH057_004521"
     CDS             31..1437
                     /gene="gndA"
                     /locus_tag="RH057_004521"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HDV3130748.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSRGKTEEVIA
                     ENPGKKLVPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCITYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLTNKELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQPAGDKGEFSEKVRRALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGKIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            1637..>1772
                     /locus_tag="RH057_004522"
     CDS             1637..>1772
                     /locus_tag="RH057_004522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130749.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     C"
BASE COUNT          463 a          408 c          457 g          444 t
ORIGIN      
        1 cgcgcggtaa ccccctgata ggagtaaaca atgtcaaagc aacagatcgg cgtcgtcggt
       61 atggcagtga tggggcgcaa ccttgcgctc aatatcgaaa gccgtggtta taccgtctct
      121 attttcaacc gttcccgtgg gaagacggaa gaagtgattg ccgaaaatcc aggcaagaaa
      181 ctggttcctt attatacggt gaaagagttt gttgaatctc tggaaacgcc tcgtcgcatc
      241 ctgttaatgg tgaaagcagg tgcaggcacg gatgctgcta ttgattccct caagccatac
      301 ctcgataaag gtgacatcat cattgatggt ggtaatacct tcttccagga caccattcgc
      361 cgtaatcgtg agctttctgc agaaggcttt aacttcattg gtaccggtgt ttccggaggc
      421 gaagaaggtg cgttgaaagg tccttccatt atgcctggtg ggcagaaaga agcctatgag
      481 cttgttgcgc cgatcctgac caaaatcgcc gcagtggctg aagacggtga gccatgcatt
      541 acctatatcg gtgccgatgg tgcaggtcac tatgtgaaga tggttcacaa cggtattgaa
      601 tatggtgata tgcaactgat tgctgaagcc tattctctgc ttaaaggtgg cctgaaccta
      661 accaacaaag aactagctca gacctttact gagtggaata acggtgaact gagcagctac
      721 ctgatcgaca tcaccaaaga tatcttcacc aaaaaagatg aagacggtaa ctacctggtt
      781 gatgtgattc tggatgaagc ggctaacaaa ggtaccggta aatggactag ccagagtgcg
      841 ctggatctcg gcgaaccgct gtcgctgatt accgagtctg tgtttgcacg ttatatctct
      901 tctctgaaag atcagcgtgt agccgcgtct aaagttctct ctggcccgca agcgcagcca
      961 gcaggcgaca aaggtgagtt cagcgaaaaa gttcgtcgtg cgctgtatct gggtaaaatc
     1021 gtttcttacg ctcagggctt ctctcagctg cgtgctgcgt ctgaagagta caactgggat
     1081 ctgaactacg gtaaaatcgc gaagattttc cgtgctggct gcatcatccg tgcgcagttc
     1141 ctgcagaaaa tcaccgatgc atatgccgaa aatccgcaga tcgctaacct gctgttggct
     1201 ccgtacttca agcaaattgc cgatgactac cagcaggcgc tgcgtgatgt cgttgcttat
     1261 gcagtacaga acggtatccc ggttcctacc ttcgccgctg cggttgctta ttatgatagc
     1321 taccgtgccg ctgttttgcc tgcgaacctg atccaggcac agcgtgacta tttcggtgcg
     1381 catacttata agcgcattga taaagaaggt gtgttccata ccgaatggct ggattaatct
     1441 gatttaacca acaataaaat tgaggcccgg cttatattgc gtcgggcttt tttgtgcacg
     1501 gaaaaccccc agctaggctg ggggttccgg aaagctttca gctttgagcc agttattaaa
     1561 accccttttg atttgttaaa acaccttgcg gtctggcaac tgcaagtgtc aaacaagaaa
     1621 tcaaaagggg gtcccaatgg ggaacgaaaa gagcttagcg cacacccgat ggaactgtaa
     1681 atatcacata gtttttgcgc caaaataccg aagacaggtg ttctacagag agaagcgtag
     1741 agcaataggc agtattttga gaaagctgtg tg
//
LOCUS       DAPHDW010000290         1721 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.297, whole genome shotgun sequence.
ACCESSION   DAPHDW010000290 DAPHDW010000000
VERSION     DAPHDW010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1721
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.297"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1267
                     /gene="speC"
                     /locus_tag="RH057_004523"
     CDS             <1..1267
                     /gene="speC"
                     /locus_tag="RH057_004523"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3130750.1"
                     /translation="DYILFDSAWVGYEQFIPMMADCSPLLLDLNENDPGILVTQSVHK
                     QQAGFSQTSQIHKKDSHIKGQQRYVPHKRMNNAFMMHASTSPFYPLFAALDINAKMHE
                     GVSGRNMWMDCVVNGINARKLILDNCQHIRPFVPELVDGKPWQSYETAQIAVDLRFFQ
                     FVPGEHWHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATILANFLRENGV
                     VPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLPSIYKQHEERY
                     AGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAYLRGEVELVRL
                     PDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPGFAPELQGVYI
                     EEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            1264..>1721
                     /locus_tag="RH057_004524"
     CDS             1264..>1721
                     /locus_tag="RH057_004524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3130751.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSIT"
BASE COUNT          413 a          411 c          451 g          446 t
ORIGIN      
        1 tgactacatt ctgtttgact ccgcatgggt tggctatgag caatttatcc cgatgatggc
       61 tgactgttcg ccgctgttgc tggatcttaa tgagaacgat ccgggtattc tggttacgca
      121 atccgtacat aaacagcagg ctggtttttc tcaaacttca caaatccata aaaaagacag
      181 ccacatcaaa gggcaacagc gttatgtacc gcacaaacgc atgaacaacg cctttatgat
      241 gcacgcctcc accagcccgt tctatccgct gtttgccgca ctggatatca acgccaaaat
      301 gcatgaaggt gtcagcggtc gtaatatgtg gatggattgt gtggtaaatg gcattaatgc
      361 ccgcaaactg atcctcgata actgtcagca tattcgtccg ttcgtacctg aactggtgga
      421 tggtaaaccc tggcagtcgt atgaaacagc gcaaattgcg gttgatctgc gcttcttcca
      481 gtttgtacca ggggaacact ggcattcttt tgaaggctat gcagagaatc aatactttgt
      541 cgatccttgc aaactgttgc tgacaacccc aggtattgat gcacgtaacg gcgaatatga
      601 agcgttcggt gtacccgcga cgattcttgc taacttcctg cgcgaaaatg gcgtagtgcc
      661 ggaaaaatgc gatcttaact ccatcctctt cctgctgact ccggcagaag atatggccaa
      721 gcttcagcaa cttgttgccc tgctggtacg cttcgaaaaa ctgcttgagt ccgacgcgcc
      781 attagcagaa gtgctgcctt ccatctacaa acagcatgaa gagcgctacg ccggttatac
      841 cctgcgtcag ttgtgtcaag aaatgcatga tttgtatgcc cgtcacaacg tgaaacaact
      901 gcaaaaagag atgttccgta aggaacactt cccacgcgta agcatgaatc cgcaagaagc
      961 caactacgcc tatttacgcg gtgaagtgga gctggttcgt ctgccggatg cagaaggccg
     1021 tatcgctgcc gaaggtgcgc ttccttatcc tccgggtgtg ctgtgcgttg ttccgggtga
     1081 aatctggggt ggtgctgttc tgcgttactt cagcgctctg gaagaaggga tcaacctgtt
     1141 gccaggtttt gcaccggagc ttcagggtgt ctatatcgaa gaacatgatg gtcgtaagca
     1201 agtttggtgc tatgtcatca agcctcgtga tgcgcaaagt accctgttga aaggggaaaa
     1261 attatgagtc aggctaaatc gaacaaaatg ggcgtcgttc agttaaccat actgacgatg
     1321 gtcaacatga tgggctccgg tatcatcatg ctgccgacaa agcttgccga agttgggaca
     1381 atttcaatta tctcctggct ggtgacagcc gtaggctcaa tggcactggc atgggcattc
     1441 gcaaaatgcg gtatgttcag ccgtaaatca ggtggtatgg gcggttatgc cgagtatgct
     1501 ttcggtaagt ccggcaattt tatggcgaac tacacctacg gtgtctcgct gctgattgct
     1561 aacgtcgcga ttgctatttc ggcggttggt tacggcaccg aactgctcgg cgcgagtttg
     1621 tcgccagtgc agattggtct tgcgaccatc ggagtgctgt ggatttgtac cgtggctaac
     1681 tttggtggtg cgcgcattac cgggcaaatc agtagcatta c
//
LOCUS       DAPHDW010000291         1712 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.298, whole genome shotgun sequence.
ACCESSION   DAPHDW010000291 DAPHDW010000000
VERSION     DAPHDW010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1712
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.298"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(39..1210)
                     /locus_tag="RH057_004525"
                     /pseudo
     CDS             complement(39..1210)
                     /locus_tag="RH057_004525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
     gene            complement(1327..1712)
                     /locus_tag="RH057_004526"
                     /pseudo
     CDS             complement(1327..1712)
                     /locus_tag="RH057_004526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          472 a          395 c          260 g          585 t
ORIGIN      
        1 tagttcaaac ttccacaacc gcagggcaag agcatgaatt aaatttgtgt taaatttttc
       61 attttgtcag accgaaatcc aggcattgcg caggatgcaa ttactttctc tatgtaactt
      121 gtatttaacg cagccttctt tctttttctc tttaccccgc ccttaaaacc tttgcaatct
      181 ctcaataaat tcagggccac tttctttatt ccagatattc ttttggctgc gtttcctctt
      241 cttattctgc ttccatcttc attcattttt acatcgagca cccaatgatg attattctcg
      301 attttccagt gagctctgat agcatgtgca aattctttag catccatatc ctttgatgaa
      361 atgtaatatc gaatgattaa accttccgct gatttatctt ctttctttta cctgaatgac
      421 aatgccacac aaagcttttt taatcccttc cattcaaatt caaaatcaca aaattcaggc
      481 gttacgttac tgataatatg taaacgtgtt tcttttcttc catggcttat ctcctgagta
      541 tcaaatgaat cgcctttata attagagaac atttttacag ggaatttttc ttcgaatgca
      601 tgatgtaatt tcccctgatt gccttttact gccagaagat aatctgcctt tttatcttta
      661 attttagaag cgatatcttt ctgacagccc atagcatcaa tggttatcaa acttttcttt
      721 aaatccagta ggttaagcaa ctctggaatg gctgtaacct cattactttt ggcttccgtt
      781 ttcacctgcc ccagtacaat accattttcg gtcgagaatg cactcaccat atggattgct
      841 ccttttcttt ttcccttatc aaaggagcct cttatggtct ttccatctat tgctataatt
      901 tcgccatcag tgatttcatg gcattcctgc atccattcaa taaacatctt ttcaaaggcc
      961 aaactgtcaa tgttactcac aacgcgtgca atggtgtcat ccaccggaat gccattagca
     1021 aaatcaccat atttctttag ccattccagt ctttcatgtc caaaatcttc aatttcctgc
     1081 cactcatcgg caccagcaat tactgcacag acggtgagga gcaaaatatc agataattta
     1141 tgtctaactt tccttgttgt cgtccatcag ggatcacaga aatataatca agcaaacttt
     1201 gaatactcat ctgggtgtcc ttttatcttt ttagttattc caaagattaa attttaatca
     1261 cccgtgagag ttaaaaccag gataaaaaga gtgacttata tcacgctttt cacgatcttg
     1321 ccctgcttta atatccaata tgttaagaag ttcagggata gttgtgatct cattggattt
     1381 ctcatctgtc ttgatctgtc cgatgaccag actgtgcatt gttgagaacg cactaatgac
     1441 atgaatcgct cccctgcagc gactcttgtc ataagaatgc cggagtgttt tccatcaatt
     1501 gcgatgacgt ctttatcatt tgaagaatgg cagtcacgca tccagttaat aaagcactcg
     1561 tgaaattttg caggactgat acaggataca accctggcaa ttgtatcgtg aacaggaata
     1621 ccattttcaa aatcaccata ttgcttcaaa aaatcgagat gagtttcccc aaaaccctct
     1681 atatcacccc agccttctgc accagaaata ac
//
LOCUS       DAPHDW010000292         1696 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.299, whole genome shotgun sequence.
ACCESSION   DAPHDW010000292 DAPHDW010000000
VERSION     DAPHDW010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.299"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..221
                     /locus_tag="RH057_004527"
     CDS             <1..221
                     /locus_tag="RH057_004527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HDV3130752.1"
                     /translation="PRKNKTAINIEYMKASIRARVEHPFRIIKRQFGFVKARYKGLLK
                     NDNQLAMLFTLANLFRVDQMIRQWERSQ"
     gene            complement(443..672)
                     /locus_tag="RH057_004528"
                     /pseudo
     CDS             complement(443..672)
                     /locus_tag="RH057_004528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012602590.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
     gene            851..1588
                     /locus_tag="RH057_004529"
     CDS             851..1588
                     /locus_tag="RH057_004529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001389182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDV3130753.1"
                     /translation="MKKVIAVSTLAMAGMFSAQTLADENKTGFYVTGKAGVSVMSLSE
                     QRFVDGEGAWADKYKGSDKSDTVFGAGLAVGYDFYQHYNVPVRTEVEFYGRGNAESKY
                     RLSYWESAGGAEFDDAQNKLSVNTLMLNAYYDFRNSSAFTPWISAGLGYARVHHKTSY
                     IYTDNSPAGSEVYSASASKYENNLAWSLGAGVKYDVTQDFSLDLSYRYLDAGDSTLTY
                     KDEDGAKYKSSVDVRSNEFMLGATYNF"
BASE COUNT          467 a          372 c          395 g          462 t
ORIGIN      
        1 atccgcgcaa gaacaaaacg gccatcaaca tcgaatacat gaaagccagc atccgtgcca
       61 gggtggagca cccgtttcgc atcatcaagc ggcagttcgg cttcgtgaaa gccagataca
      121 aggggctgct gaaaaacgat aaccaactgg cgatgttatt caccctggcc aacctgtttc
      181 gggtggacca aatgatacgt cagtgggaga gatctcagta aaaaccggaa ataacgccag
      241 aaatggtgga aaaaatagcc taaataggct gattcgatgt gtttgcggga aaaaaatcgg
      301 cccagatccg agaaatttta atcagcgagt cagcttggga agaaatgacc tgcttattcg
      361 caccttccct agtgaaatag ccaaccaatc gtccctataa ggcaggttaa atagttttaa
      421 tttagcaaat ttgataaaca actcactcgc cattaccccg attgatcttc gcatagtcaa
      481 acggcgtaat cgctctctcc cgattggcat ccagaaaatc tgcccaccat tgcagcatca
      541 gcttgcgctc atccagatgc tccgctttat ggatatacgc tgcctgcact gagttacgtc
      601 catgtggctc atctgacgtt cagccgcttt atctcgtgct gttctggtct caatacttcc
      661 atgccgttgc aattacctct taaaccattc cttatcattt tcatgtcttc gataagaaat
      721 cttcatcatt catgatcgat tttgtcgatc gattcgatcg aattttctct ttttaccttg
      781 tcttttgcat ggttttaaaa tgagcaacct tctttatcgg gtggggtaag aaccaacgga
      841 gatgattgaa atgaaaaagg ttattgctgt ttcaacactt gcaatggcag gcatgttttc
      901 ggcccagact ctggctgatg agaacaaaac tggcttttat gtgaccggta aagccggtgt
      961 ttctgttatg tcactttcag agcaacgttt cgttgatggt gaaggggcat gggcggataa
     1021 atacaaaggc agtgacaaaa gtgatacggt ttttggcgca ggccttgcgg tgggatacga
     1081 tttttatcag cactacaatg tgccggtgcg tactgaagtg gagttttatg gccgcggtaa
     1141 cgctgaatca aaatatcgtc tgagttattg ggaaagtgcc ggtggtgccg agttcgatga
     1201 tgcacagaac aagctcagtg taaacacgct gatgctgaat gcgtattatg atttcagaaa
     1261 cagcagtgca ttcacgccat ggatatctgc tggtctgggt tatgcacggg ttcatcataa
     1321 aacttcgtac atatataccg ataacagccc ggcaggcagt gaagtttatt cagcttcagc
     1381 gtcaaaatac gaaaacaacc tggcatggag cctgggtgcc ggtgttaaat acgatgtgac
     1441 gcaggatttc agccttgacc tcagctaccg gtatctggat gcaggggatt ccactctgac
     1501 ttataaagat gaggatggtg ctaaatataa atcctctgtt gatgtcagaa gtaacgagtt
     1561 tatgttaggc gcaacgtata acttctgaca gccatttcta cctgcgcttt ggggcacagt
     1621 aaagtgcagg tatgacaaat tgtcaacaac agatatcccg atgatattct ccctctggca
     1681 actaataatt ttagat
//
LOCUS       DAPHDW010000293         1686 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.300, whole genome shotgun sequence.
ACCESSION   DAPHDW010000293 DAPHDW010000000
VERSION     DAPHDW010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.300"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..527
                     /locus_tag="RH057_004530"
     CDS             <1..527
                     /locus_tag="RH057_004530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HDV3130754.1"
                     /translation="VKGINREQLQTMITESGIALDISCDNTPRQQVIGGTQASLNEFA
                     TLLMAAGYEPVKLGVSGAWHTRLMEDGVQAMRDYLAGLDIASPEHQVLMNVTAKSEVA
                     PSIIKENLSLHLTHTVKWTESLDIFLNMPTPVAFLEISNKPYLGNMLNDFAGVDQQRV
                     MHCRKAFSDAKVFK"
     gene            524..1510
                     /locus_tag="RH057_004531"
     CDS             524..1510
                     /locus_tag="RH057_004531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130755.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRYAFLGRLFLQEIDLFFSSENALSTFFAKPLWLRLFILLVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRCLWHNDFMMACDMYFRWGHFNK"
BASE COUNT          414 a          328 c          403 g          541 t
ORIGIN      
        1 cggttaaagg catcaaccgc gaacaactgc agacgatgat tacggaatca ggcattgcgc
       61 tggatattag ctgtgataac acgccgcgcc agcaggttat tgggggaacg caggcctcgt
      121 tgaatgaatt tgccacgctg ctgatggccg ctggatacga accggttaaa ctcggtgtga
      181 gcggtgcctg gcacacgcgt ttgatggaag acggcgttca ggcaatgcgc gattatcttg
      241 ccggactgga tatcgcttcg ccagagcatc aggttctgat gaacgtaaca gcaaaaagcg
      301 aggtcgctcc gtcaattatc aaagaaaatc tctcattaca cttaacccat acggtgaaat
      361 ggactgaatc tctcgatata tttctgaata tgccaacccc ggtagctttc cttgaaataa
      421 gtaataagcc ctatttgggg aatatgttaa atgattttgc tggcgtggat cagcaacgag
      481 ttatgcattg tcgtaaagca tttagtgatg caaaggtatt caaatgattc ctgattattt
      541 aacttttatt cgctttcagg ataaacgaaa tctgatatac atttatgcta ttggacttat
      601 tctgataggc ttttattgga aaaatgcagg atttactttt ccatcagagg atattggtgt
      661 cgttagtggg attctggccc tggtgctgta taattttatt tttgatctca aggcgtactg
      721 ggcttataaa tgcgtcacga agaatatcga tttttcgtgg tttaagaaaa agcagaacca
      781 caaaatagaa ttatttctta cacaacctct ggtggcagga tttctgtcgt taatcatgtt
      841 gagtgcaatg agttgggggc tataccagct tctaccctcg ttatatgcgc tgttcctgat
      901 ttcgttactt gggccgttgg tcatctttct gctgtttcgg atgatccgca ccagttatgt
      961 caagcaggtc gctatttcag tagcgaaaaa agtaaaatat aaaagtctga ctcgctatgt
     1021 gctgctttcg gtgtgcatct caacggttgt taacctgctt actatcagcc cgttacgtaa
     1081 cagtgattct tttgtgacag aggggcagtg gttaacgttt aaatcgataa ttgcattgct
     1141 cattctttgt ggcgtagtgt tggcgattaa tctgtttttt ctgcgcttct ccaagcggta
     1201 cgcttttctg ggcaggcttt ttttgcagga aatcgatctg tttttctcca gtgaaaatgc
     1261 gttgtcgacc ttttttgcca agccgctttg gcttcggtta ttcatattgc tggttattga
     1321 aatgatgtgg attacgctgg tgtcggtatt ggcaacgctt gtagaatggc ggatttggtt
     1381 tgaagcctat tttttactct gctatgtacc gtgcttaatt tactattttt tccattgtcg
     1441 atgcctctgg cataacgatt ttatgatggc atgtgacatg tatttccgtt gggggcattt
     1501 taataagtga ggaagttaca ggaagtgacc agataacgca tatatgttct ctactatctt
     1561 gcgcattttg attgttgact gagtaaccag acagttgatg tgcacaattt cccctcgccc
     1621 taacagacgt gggcgggggc acccggtgtg tgcaatatac gaaaaaaaag cccgtacttt
     1681 cgtacg
//
LOCUS       DAPHDW010000294         1686 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.301, whole genome shotgun sequence.
ACCESSION   DAPHDW010000294 DAPHDW010000000
VERSION     DAPHDW010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1686
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.301"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(641..880)
                     /locus_tag="RH057_004532"
     CDS             complement(641..880)
                     /locus_tag="RH057_004532"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130756.1"
                     /translation="MEREFPEALERMKERLPLYLIMVLENQYNRLNELDSLIEDIEKQ
                     LTSVAKQNETCKRLLDIPGVGPLIATAAVAIIGEA"
BASE COUNT          452 a          407 c          367 g          460 t
ORIGIN      
        1 ttgaagacag atatgcctgc agacctggag ggccgtggcg ggatatgggg ctttatatga
       61 atgcatcccg tttacacagc ttccattgtt ctggcattga cggtaggttg cagcctttat
      121 atccggcatt attgcagcca taaagctgcg agcccctatg taattcgctg actcacactt
      181 catttactgc gcgggattca agccaaaaaa acattcaggt ttagtgagtc ccggtcttac
      241 ctgtctttgt catcactacg gctgccctcg caacctttca gcatccccaa ttttgttaag
      301 agttcattgt tgaacggtat cagcgattaa tatggtctga tactgacatg gttcccgtca
      361 tacttacgac catgtgcggc tattgcccac actgttcgtg ccagcttgtt tgccatggcg
      421 acgttgccac gccgaccgga cgacgttttt tcagttcggt tatccacggc ccaggttctc
      481 tggtcagtaa tgctgccgcc cttgcaccat gaataaacag ggttcgcaga tatttatcac
      541 cacgtttgct tatccccggc aggcggattt tccctcatga gccagtctgt cttggctcca
      601 gaccagcata agccgcgaac tctcgccctg atttaaacgc ttatgcttcc cctatgatag
      661 ccaccgctgc cgtcgcaata agtggcccaa cgccgggaat atccagcaac cgcttacagg
      721 tctcattctg tttcgccacg ctggtaagct gcttttcaat atcctctatc agtgagtcca
      781 gctcattcag tcggttgtat tggttttcca gaaccataat gagatacagt ggcagtctct
      841 ctttcatccg ctccagcgct tcagggaact cccgttccat cgctgcccgg cctttgtgga
      901 tggttttacc aaactccagt agcgtcccgt gcagggcatt aacggtctaa catcgtggcg
      961 ttgacaccaa taaaaggaga aataaaaata cctaccataa taaatggcag ataaaactgt
     1021 gataagggag gaaaacaggt aggtgtatgc tctcctgtcg gtcatcaggt aatctgatac
     1081 aagaagatac tgttcagtta aaccgcggga gataatctgt tatgaaatat gtgcgtcact
     1141 tctcagaaat cgtgtaataa caaacaaaat taatcaaaag aaattcatcg caatgatatc
     1201 ccaatatgcc tgtgagcata taaaaaaagc ccgaggacac ttacgggctg attgagcgac
     1261 attgatatta agtaatgatt ttttctttaa attctacgat aaaggacgtg gctttaactt
     1321 tgtcggaaat cacaacaaca caacgaataa aatatagcga gggggagacg tgttcatggg
     1381 cattcgccag ggatgaataa aacagcaaca aaaaaatgcc cgtacctctc gtacgggcca
     1441 gcgtcacaca ttatcatgga gttgtcagcg ggatttatta ttagctttga tggaatatat
     1501 aatcagatac tggcgttgtt ctgtgatttt tatcacacct ttaaatgtat taaatcagcc
     1561 acacatcttt atgtgttcac cagtccaggt aataccggat gcccctgcat ccacatccgt
     1621 aacagcaaac ctgaatcatt gtagtggaat aacctctcag accagtgata cggatgggta
     1681 acagga
//
LOCUS       DAPHDW010000295         1680 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.302, whole genome shotgun sequence.
ACCESSION   DAPHDW010000295 DAPHDW010000000
VERSION     DAPHDW010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1680)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1680)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1680
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.302"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            91..519
                     /locus_tag="RH057_004533"
     CDS             91..519
                     /locus_tag="RH057_004533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130757.1"
                     /translation="MTNKKTQFLNTKDAKHSTSKCNLLPQELRIHCVSVRLNNTELAK
                     LNTLREHYSKGEWLRMASLQKLPPVIPAINTKAWIALTDISQKLNRIAIHIDGKSKDS
                     KLTHTELFAVKRQLEELRQHLLNADIWSKPYEGYAEDQKG"
     gene            488..1648
                     /locus_tag="RH057_004534"
     CDS             488..1648
                     /locus_tag="RH057_004534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HDV3130758.1"
                     /translation="MKGMQKIKRGKSFAGVVQYALKPGSHHKSNPIVIGGNMLGGSAL
                     ELISEFNGTKQLRPDVQKAVWHNSLRLPEGETLSNDQWVSIADDYMKKMGFSDTHLRC
                     YVLHDDEGQHIHIIASRIDIAGGKLYLGRNENLISTRIISELEMSHGLTVTKTASPPS
                     QAQPKRKRVSRNEQMLSARTGVPCPKEALQQIIDKSMTDKPDLLTFVKRLEDSEVGCK
                     ANIASTGKMNGFSFEYRNIAFKASQLGKAYSWVNLQKQLNYNQNHLEVLRATKEAIPI
                     PVPVPVPVPVPVPIESTARTTAQSESIGEKIAEIEQRLREDRRNEIVEKILQKNAVKQ
                     QKHLRLIGWLPFLRRLAELLRSYGKSILHKPLPGFSKLDATRTLHKGRKIHL"
BASE COUNT          549 a          379 c          346 g          406 t
ORIGIN      
        1 gcttaacatt gacaactaat aattatttac tataacaaga gataaagtta gttaatcact
       61 tttaaagcat gacatataaa aggagtttat atgacaaata aaaaaacgca gtttttgaac
      121 actaaggatg caaaacattc aacctcaaag tgtaatttac taccacagga attacgaatt
      181 cattgtgtta gtgttcgtct taacaatact gaattggcca agcttaatac attgagggag
      241 cattattcga aaggagagtg gcttcgtatg gcttcccttc aaaaattgcc tccagttata
      301 ccagctatta acaccaaagc ctggatagct ttaactgaca tatcgcaaaa gctaaatcga
      361 attgcgatcc atatcgatgg taagagcaaa gacagcaagc ttacgcatac agagcttttc
      421 gcagtgaaac gacaactgga agagcttcgc cagcacctgc taaatgctga catatggagc
      481 aaaccctatg aagggtatgc agaagatcaa aaggggtaaa agcttcgctg gtgtcgtgca
      541 atatgcattg aagccaggtt cacatcataa aagtaatcca atcgtcattg gcgggaacat
      601 gttaggtggc tctgctcttg aattgatttc cgaattcaat ggcacaaaac aacttcgccc
      661 agatgttcag aaggccgttt ggcacaactc gttacgattg cctgagggag aaacattatc
      721 gaatgaccaa tgggtgtcca tagctgacga ctacatgaag aagatggggt tcagtgatac
      781 tcacctccgt tgctatgtct tacatgacga cgaaggccag cacatacaca ttatcgccag
      841 cagaatcgac atagccggtg ggaagctcta cttgggtagg aacgaaaacc ttataagcac
      901 acggatcatt agtgaactcg aaatgtctca cggtctgaca gtgaccaaaa ctgcatctcc
      961 cccctcgcaa gcacaaccga agcggaagag agtgtcccgt aacgaacaaa tgctatcggc
     1021 ccggactgga gttccctgcc ctaaggaagc tctccaacag atcattgata aaagtatgac
     1081 tgataagccc gaccttctta cttttgttaa acgactcgaa gattcagaag tcggctgtaa
     1141 ggccaatatt gcttctaccg gtaagatgaa cggcttctct ttcgaatacc gcaatatagc
     1201 tttcaaggca tctcaacttg gaaaggcata ctcatgggta aatcttcaaa agcagcttaa
     1261 ctacaaccaa aaccacttag aagttcttcg agcgacaaag gaagctatcc ccatccctgt
     1321 tcctgttcct gttcctgttc ctgttcctgt tcctatagaa tcgacagcaa gaacgactgc
     1381 ccagagcgaa agcataggcg aaaaaattgc tgaaatagaa cagcgactca gagaagacag
     1441 acgaaacgag atcgttgaga agattcttca aaagaatgca gttaaacagc aaaagcatct
     1501 aaggcttatc ggctggcttc catttcttag aaggcttgca gagcttctcc ggagctatgg
     1561 taagagcatt cttcacaagc ccctccctgg attctcaaaa ctcgatgcca cacggactct
     1621 acacaaagga agaaaaattc atttatagac aaaaatttac cgcctatccc tgacagcaat
//
LOCUS       DAPHDW010000296         1649 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.303, whole genome shotgun sequence.
ACCESSION   DAPHDW010000296 DAPHDW010000000
VERSION     DAPHDW010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1649
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.303"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            123..731
                     /locus_tag="RH057_004535"
     CDS             123..731
                     /locus_tag="RH057_004535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HDV3130759.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVIRNKKPYVFEAVGPVKYTPLKQWIAHGEKGKYVVRRVEGGLSVEQQQK
                     LAQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            876..1551
                     /locus_tag="RH057_004536"
                     /pseudo
     CDS             876..1551
                     /locus_tag="RH057_004536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          433 a          417 c          389 g          410 t
ORIGIN      
        1 atattgcaat ttattgaatt tgcacgaact tgtaggccgg ataaggcgtt cacgccgcat
       61 ccggcataaa caacgagcac gttgtctgcg acccaccgct ttttatacat ggacgtttaa
      121 ctatgaaaaa caggctgctg atcctcagcc tgctggtttc tgtacctgcc tttgcctggc
      181 agccgcaaac tggcgacatc atctttcaaa tctctcgctc atcgcaaagt aaagcgatcc
      241 aactggcaac ccattccgat tacagccaca ccggtatgct ggtgatacgc aacaaaaagc
      301 cctacgtttt tgaagcggtc ggcccggtga aatacacccc gctcaagcag tggatcgccc
      361 atggtgaaaa gggcaaatac gtcgttcgcc gcgttgaagg cggactgagt gttgaacaac
      421 agcaaaaact ggcgcaaacg gcaaaacgtt atctcggtaa accgtatgat ttcagcttct
      481 catggagcga cgatcgccag tactgttcgg aagtggtgtg gaaggtttac cagaacgcgc
      541 tgggaatgcg cgtgggcgaa cagcagaagt tgaaagagtt tgatctcagc aacccgctgg
      601 tccaggcgaa gctcaaagaa cgctacggta aaaatattcc gcttgaggaa acggtagttt
      661 caccacaagc cgttttcgac gcgccacaac tcaccacggt tgccaaagag tggccgctgt
      721 tttcgtggta atggatgatg aaaaaaataa ttcccgccgt agtctgtttg ttgctttgta
      781 gttgcacgac ctttccgcaa gctgtcgctc cggtaaacgg tgatgcctcc aattagttga
      841 attaaggata taatactcgc ttttaaggtt gtttcatggt cacagttaac cttcattgcc
      901 cccgttgcca gtccgttcag gtttaccgtc acgggcagaa cccaaaaaga catgatcgat
      961 tccgcggccg tgactgtcac cgggtatttc agctcagtta ctgttacgag ccccgtaaac
     1021 cgggagttaa agaccaaatc gctgaaatga ctttcaacgg tgccggggtt cgcgatactg
     1081 cccgtacgct gaaaatcggc attaatacag tcatccgaac tttaaaaaac tctcgccacg
     1141 aagaataacg tcatccccgg tcgctcatgc tgatatggca ctcatctgca aacttagtca
     1201 gcaatggagc tttgtcggca gtaaagcgcg acagcactgg ctctggtacg tgtacaacac
     1261 gaaaactggt ggtgttctgg cctacacttt tggcccccgg actgacgaaa cctgtcgtga
     1321 attgcgggcg ctgcttaccc ttttaccatc ggcatgttga ccactgacga atggggtagt
     1381 tacgctagag agttaccgaa ggagaagcat ctgactggta agatttttac tcagcacatt
     1441 gagcgtaaca atctgaccct gagaacccga atcaaacgtc tggcccgcag aaccatctgt
     1501 ttctcgcgtt ctgtagaact tcatgaaaaa gtcatcggtg cttttatcga aaatatatgt
     1561 tctactaatt ggagtcatca cccttctgat aagctttcct tggatgatta ccagactgaa
     1621 tacacagcca aagattttta cctcattct
//
LOCUS       DAPHDW010000297         1640 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.304, whole genome shotgun sequence.
ACCESSION   DAPHDW010000297 DAPHDW010000000
VERSION     DAPHDW010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.304"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1010..1612
                     /gene="fimB"
                     /locus_tag="RH057_004537"
     CDS             1010..1612
                     /gene="fimB"
                     /locus_tag="RH057_004537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
                     /protein_id="HDV3130760.1"
                     /translation="MKNKADNKKRNFLTHSEIESLLKAANTGPHAARNYCLTLLCFIH
                     GFRASEICRLRISDIDLKAKCIYIHRLKKGFSTTHPLLNKEVQALKNWLSIRTSYPHA
                     ESEWVFLSRKGNPLSRQQFYHIISTSGGNAGLSLEIHPHMLRHSCGFALANMGIDTRL
                     IQDYLGHRNIRHTVWYTASNAGRFYGIWDRARGRQRHAVL"
BASE COUNT          492 a          290 c          356 g          502 t
ORIGIN      
        1 aaaatcacgc aatccataca acaaaaccag atttgcaatt cgtgtcacaa aatatgtcga
       61 tctttttcta agaggaagat gccatgtgaa gccagacgaa cactttcggt ggtcttcaaa
      121 aactaaagat ctttgtttaa ctatttgttt tataaataat ttatttagag tctaaacaag
      181 ggaagctttg caagctaact cagtgagctt ggtgaaaatc agtgtttacc cgccatcagg
      241 ctgagcataa ttctcatcat gaaatatgtt tcctggtttg tggcttgtaa ctggtcactt
      301 ctgaagtcga tctggagagg cttgttgatg ttggtgtttt caggatgatg tttcacttag
      361 tttgtttgcc gtatcgcccg gcgaatggct gtgattgagg aaggtttaag tcgtagtgac
      421 caaagctata tttaccaacg aatgtagatg aaaaaatcat ctcctgcgtt cccccatatc
      481 tccaggataa aaaggaatgt aacaatctca tggcgtaagc tgacgaatca gccaggaata
      541 atcgctaggg acctaagaat tagcatgata atagccacta agaaattact gcgctccatg
      601 aaatagccat tttgtggcaa atggagttga ctaataatgt catatgtgag acggctagtt
      661 gaacgaatat taaattttgc tgaatttttt atgttgattt tacttgttac agaacatatc
      721 acatgatata tagataagat tagttgcatt aatgatgagg gttattatta gattcgtatc
      781 cgattgataa atatataaag gtacatagca tgcaagagca tggcgtttgt atggcaacgt
      841 tattataatt aactgttgct actccattta agttcactca gaagaactgg tccacttacg
      901 ttagttatta agcaaacgtt cgcttttata aacataatca ggataaaaat gttggattat
      961 tgctaaccca gcacagctag tgcgcgtctg taattataag ggaaaaacga tgaagaataa
     1021 ggctgataac aaaaaaagga acttcctgac ccatagtgaa atcgaatcac tccttaaagc
     1081 agcaaatacc gggcctcatg cagcacgtaa ttattgtctg actttgcttt gttttattca
     1141 tggtttccgg gcgagtgaaa tttgtcgatt gaggatttcg gatattgatc ttaaggcaaa
     1201 gtgtatatat atccatcgat taaaaaaagg cttttcaaca acgcatccgc tattgaacaa
     1261 agaagttcag gctttaaaaa actggttgag tatccgtact tcgtacccgc atgctgagag
     1321 cgagtgggta tttttatctc gtaagggaaa tccgctttct cggcaacagt tttaccatat
     1381 tatctcgact tccggtggta atgccgggtt gtcactggag attcatccgc acatgttacg
     1441 ccattcgtgt ggttttgctt tggcgaatat gggaatagat acgcgactta tccaggatta
     1501 tcttgggcat cgcaatattc gtcatactgt ctggtatacc gccagcaatg cagggcgttt
     1561 ttacggcatc tgggatagag ccagaggacg acagcgtcac gctgttttat agagatagtt
     1621 gagataccag ggatggtgtt
//
LOCUS       DAPHDW010000298         1636 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.305, whole genome shotgun sequence.
ACCESSION   DAPHDW010000298 DAPHDW010000000
VERSION     DAPHDW010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1636
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.305"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..238)
                     /locus_tag="RH057_004538"
                     /pseudo
     CDS             complement(1..238)
                     /locus_tag="RH057_004538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            complement(341..1467)
                     /locus_tag="RH057_004539"
                     /pseudo
     CDS             complement(341..1467)
                     /locus_tag="RH057_004539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027427.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          455 a          367 c          231 g          583 t
ORIGIN      
        1 aggatacaac tctggcaata gtatcgtgaa caggaatacc attttcaaaa tcaccatatt
       61 gcttcaagaa atcgagatgt gtttccccga aatcctctat atcttcccaa ctttctgcac
      121 cagaaataac ggcaaaaata gtcaatagta ggatgcctga caatttatgc tctactttcc
      181 aggcttatct gtaatcggga ataatagaaa tatgttccat caatttttta agttccattt
      241 gttcacctta tttatgtaag gagtatttga tcatgtataa gcaataaaaa acagcttcag
      301 gtaatgagga atatctcaat ttttaaacat aaaatgcgaa ttatttagta caaaaagtga
      361 acttctgatg atctttccct gtgtttaacg cagccttctt tcttttcctc ttcaccctta
      421 atgtctttgc aatctcttaa taaattcagt gccatcttct ttattccaga tattatttag
      481 gctgcgtttc ctcttcttat ccggctggca tcttcattca tttttacatc taacacccaa
      541 tgaagactgt gctcgatcag ccagtgcgct ctgatagcat gtgcaaattc tttagcatcc
      601 atatcctttg atgaaatata atatcggatg cttacacctt ctgctgattt atcttctttc
      661 ttctgcctga atgacaatgc tacacaaagc ttttttaatc ccttccattc gaattcaaaa
      721 tcacaaaagt tcaggcgtta cgttactgac aatatgcaaa cgtgtttctt ttcttccatg
      781 acttatctcc tgcgtactaa acgaatcgcc tttataatta gaaaacacat ttacaggaaa
      841 tttttcctcg aatgcatgat gtaatttccc ctgattgcct tttactgcca gaagataatc
      901 tgctttttta tctttgatct tcgaagcgat atctttctga cagcccatag catcaatggt
      961 tatcaaattt ttctttaaat acagtaggtt aagcaactct ggaatggctg taatctcatt
     1021 acttttggct tccgttttca cctgccccag tacaacacca ttttcgttcg agaatgcact
     1081 caccatatgg attgctcctt ttctttttcc cttatcaaag gagcctctta tggtctttcc
     1141 atctattgct ataatttcac catcagtgat ttcatggcac tcctgcatcc attcaataaa
     1201 catcttttca aaggccaaac tgtcaatgtt actcacaacg cgtgcaatgg tgtcatcgac
     1261 cggaatgcca ttatcaaaat caccatattt ctttagccat tcaagtcttt catgtccaaa
     1321 atcttcaatt tcctgccact catcggcacc tgcaattact gcacatacgg tgagaaacaa
     1381 aatagcagat aatttatgtt taacctttcc ttgttgtcgt atatcaggga tcactgaaat
     1441 ataatcaagc aaactttgaa tactcacctg ggtccttttt tatcttttta gttattccaa
     1501 aggttaaatt ttgatcacct gtaggcgtta aagccaagat gaaaatcgtg atttacatca
     1561 caattttcat gatcttgccc tgccccgtcc cttgctctca tgtctttctg tatgtatttt
     1621 atatttttct ctttct
//
LOCUS       DAPHDW010000299         1622 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.306, whole genome shotgun sequence.
ACCESSION   DAPHDW010000299 DAPHDW010000000
VERSION     DAPHDW010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.306"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(162..815)
                     /gene="ribB"
                     /locus_tag="RH057_004540"
     CDS             complement(162..815)
                     /gene="ribB"
                     /locus_tag="RH057_004540"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HDV3130761.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(919..1067)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            1189..1479
                     /gene="ubiK"
                     /locus_tag="RH057_004541"
     CDS             1189..1479
                     /gene="ubiK"
                     /locus_tag="RH057_004541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HDV3130762.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRMSELENRSTEIKKQPDPETLPPTL"
BASE COUNT          464 a          404 c          358 g          396 t
ORIGIN      
        1 ttatatgatt ttgatcataa tgaaaaagaa tgcctgatat taatgaaatt ttctcataga
       61 aagagaagcg gaattaacat cttgcatagc ggggcaaaat aaaaaccgaa gccagctaca
      121 acttcggttt cttgattaag gcagtaatta agcagcggtt ttcagctggc tttacgctca
      181 tgtgcctgac ggtatgccac caggtcttca atagtcacga gcgccatatt gtgtttattg
      241 gcaaactcaa tgcactctgg tgcacgcgcc atcgtgccat cgtcattagt cagctcacac
      301 agcacaccag ccggtttaaa gcctgccagc gtcatcaaat caatagtcgc ttcagtatga
      361 ccgccacgcg tcagtacacc acctgcctgc gcgcgaagtg ggaaaacgtg gcccgggcga
      421 ttcagatcag aaggttttgc gccatcggca atcgctgcgc gaacggtcgt aatacgatca
      481 gcggcagaaa caccggtagt cacaccttca gccgcttcaa tggtcacggt aaaaccggtg
      541 ccataggcgc tggtgttatt ttctaccatc attggcagat caagttgttt acggcgatct
      601 tcagtgatgc acaggcaaac aataccgcta ccgtggcgaa tggtcagcgc catctgctca
      661 acggtcatgg tttctgccgg gaagatcata tcgccttcgt tttcacggtc ttcatcatca
      721 agcaccatta caccgcgtcc ttcacgcagc gcagccagtg cattttcaac acgttcgaaa
      781 ggcgtaccaa aagaggaaag tagcgtctga ttcatggtaa aaaaacctca ctaaaattat
      841 ggttaccaga atcagggcag tcttaggagt ggcggcatat agccaaaata acgtgagcgg
      901 gtccatgccc gacagaatcg ttactctctc ccatccggac tctaaccgtc ggccccggaa
      961 ttacaccgga tctgctgtcc tttgagttcg cacccaaagc gctcgcgggc tttcaactga
     1021 gttgatttac cgccggtggg gaatttcgcc ccgccctgag aataagcgga ttcactataa
     1081 cgctaatgat tagcggcagc aacgcatagc ttcacataat tctggtttat gacttaccct
     1141 tatcgcacta caatggtact caacacctat cagtacaggg aaactacaat gattgacccg
     1201 aaaaaaattg agcaaatcgc tcgccaggtt cacgaatcaa tgcctaaagg aatcagggag
     1261 ttcggggaag atgtggagaa aaaaatccgc caaaccctac aagctcagct gacgcgcctc
     1321 gaccttgtaa gccgtgaaga gttcgacgtc caaacgcaag tcctgttacg gactcgtgag
     1381 aaactggcgc tgcttgaaca aagaatgagc gaactggaaa atcgcagtac tgagattaaa
     1441 aagcagccag atccagagac tctcccccca acgctgtaaa catttgtctt taatgcactg
     1501 taaattatga attaatcctc taatttacag tgctttctat taccttatcg cttaattcca
     1561 ttcagaatat catcacaaaa atgaatgagt gcaattgaaa atataattgt tatgtatttt
     1621 tt
//
LOCUS       DAPHDW010000300         1609 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.307, whole genome shotgun sequence.
ACCESSION   DAPHDW010000300 DAPHDW010000000
VERSION     DAPHDW010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.307"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1085
                     /locus_tag="RH057_004542"
                     /pseudo
     CDS             1..1085
                     /locus_tag="RH057_004542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1182..>1609
                     /locus_tag="RH057_004543"
     CDS             1182..>1609
                     /locus_tag="RH057_004543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3130763.1"
                     /translation="MTHSTIKQLTIKAILEYIEDNIEVKSIDIESIVCFSGYSRRYLQ
                     SLFKSHMGVPIGRYIQLRRVSRAAVLLRLTSLSLASIAGKLCYDSQQTFTREFKKNSG
                     FTPLQYRNSNLWTFKNLTGPKEPSIVIPKPKLCYLDENKF"
BASE COUNT          459 a          351 c          425 g          374 t
ORIGIN      
        1 tggcgtaagc aataccagga aggaagtctt actgctgtgg ccgccggaga acaggttgtt
       61 cctgcctctg aacttgctgc cgccatgaag cagattaaag aactccagcg cctgctcggc
      121 aagaaaacga tggaaaatga actcctcaaa gaagccgttg aatatggacg ggcaaaaaag
      181 tggatagcgc acgcgccctt attgcccggg gatggggagt aagcttcgtc agccgttgtc
      241 tccgggtgtc gcgtgcgcag ttgcacgtca ttctcagacg aaccgatgac tggatgaatg
      301 gccgccgcag tcgtcacact gatgatacgg atgtgcttct ccgtatacac catgttatcg
      361 gagagctgcc cacgtatggt tatcgtcagg tatgggcgct gcttcgcaga caggcagaac
      421 ttgatggtat gcctgcgatc aatgccaaac gtgtttaccg gagcatgcgc cagaatgcgc
      481 tgttgcttga gcgaaaacct gctgtaccgc catcgaaacg ggcacataca ggcagagtgg
      541 ctgtgaaaga aagtaatcag cgatggtgct ctgacgggtt cgagttccgc tgtgataacg
      601 gagaaaaact gcaagtcacg ttcgcgctgg actgctgtga ccgtgaggca ctgcactggg
      661 cggtcactac gggcggcttc aacagtgaaa cagtacagga cgtcatgctg ggagcggtgg
      721 aacgccgctt cggcaacgag cttccggcgt ctccagtaga gtggctgacg gataatggtt
      781 catgctaccg ggctaatgaa acacggcagt ttgcccggat gttggggctt gaaccgaaga
      841 gcacggcggt gcggagtccg gagagtaacg gcatagcaga gagcttcgtg aaaacgataa
      901 agcgtgacta catcagtgtc atgcccaaac cagacgggtt aacggcagca aagaaccttg
      961 cagaggcgtt cgagcattat aacgaatggc atccgcatag tgcactgggt tatcgctcgc
     1021 cacgggaata tctacggcag caagccagta atgggttaag tgataacagg tgtctggaaa
     1081 tataggggca aatccatgca ttgtgtgctg cttgcgcaca ataagctaat gcgaacgtat
     1141 agtaaccaca ttagacttta tgcgtctaaa tgaggttaat gatgacgcac agcacaatta
     1201 agcaattgac aataaaagca attctcgaat acatcgaaga caatattgag gttaaaagta
     1261 tcgatattga atcgatagtc tgtttttctg gttacagtag gagatatttg caatctctgt
     1321 ttaagtctca catgggagta cccataggga ggtatataca actcagaaga gtttcaaggg
     1381 ctgcggtatt attacgattg acatcattat cattggccag tattgcaggc aaactctgtt
     1441 atgattctca acaaacattt acaagagagt ttaaaaaaaa ctcaggtttt acgcctttac
     1501 aatatagaaa cagtaatcta tggaccttta aaaatcttac cggtccaaaa gaaccatcaa
     1561 ttgtcatacc caaaccgaaa ctttgttatc ttgatgaaaa taaatttaa
//
LOCUS       DAPHDW010000301         1606 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.308, whole genome shotgun sequence.
ACCESSION   DAPHDW010000301 DAPHDW010000000
VERSION     DAPHDW010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1606
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.308"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..124
                     /locus_tag="RH057_004544"
     CDS             <1..124
                     /locus_tag="RH057_004544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage repressor protein CI"
                     /protein_id="HDV3130764.1"
                     /translation="ITRLPGGRIFVEGGNRAFECKIEDVEIIGKIISLTIKYVR"
     gene            140..331
                     /locus_tag="RH057_004545"
     CDS             140..331
                     /locus_tag="RH057_004545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001513672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130765.1"
                     /translation="MLGKVFFVVLSCSLLLNPLTTYAKNYPCSGKKGGVSHCTSDGKF
                     VCNDGTISKSKKICTKNSR"
     gene            518..1534
                     /locus_tag="RH057_004546"
     CDS             518..1534
                     /locus_tag="RH057_004546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HDV3130766.1"
                     /translation="MAVRKLTTGKWLCECYPAGRSGRRVRKQFATKGEALAFERHTME
                     ETEAKPWLGESVDRRTLKDVIELWFKLHGKSLTAGQHVYDKLLLMVDALGNPLATDLT
                     SKMFAHYRDKRLTGEIYFSEKWKKGASPVTINLEQSYLSSVFSELSRLGEWSYPNPLE
                     NMRKFTIAEKEMAWLTHEQIVELLADCKRQDPILALVVKICLSTGARWREAINLTRSQ
                     VTKYRITFVRTKGKKNRSIPISKELYEEIMALDGFNFFTDCYFQFLSVMEKTSIVLPR
                     GQLTHVLRHTFAAHFMMSGGNILALQKILGHHDIKMTMRYAHLAPDHLETALRFNPLA
                     TLEV"
BASE COUNT          486 a          347 c          359 g          414 t
ORIGIN      
        1 cattactcgt ttacccggtg gaagaatctt cgtcgagggt ggaaacagag ccttcgagtg
       61 caagatagaa gatgttgaaa taattgggaa aattataagt ttaacaatta agtacgttag
      121 gtaataccgg gaggaaacta tgcttggtaa ggtatttttt gtggttttat catgctcttt
      181 gttattaaac ccactaacta cctatgctaa aaattatcct tgttctggga aaaagggagg
      241 tgtctctcac tgtacctccg atggaaagtt cgtttgcaat gatggaacta ttagtaaatc
      301 aaaaaaaatc tgtactaaaa actcgcgata aattttgctt ttatatctgc gcctaatata
      361 acaatgagcc gcaggctaac cgcaaaagtc acatactcac atagcaaaaa atagctaact
      421 tcattatggc ttcagtgaga tgtatggtcg caggatttca tacattgaca ctggttatac
      481 atacagtaaa aatgctctct actggagggc attttttatg gcagtacgaa aactcaccac
      541 aggaaaatgg ctttgcgaat gttaccccgc cggacgtagt ggacgtcgtg tgcgtaaaca
      601 attcgccacc aaaggcgaag ctctggcttt tgagcgtcac acgatggaag aaaccgaagc
      661 aaagccctgg ctgggcgaat cagtggatcg tcggacattg aaagacgtga ttgagctatg
      721 gttcaaacta catggtaaat ctctgacagc tgggcagcat gtctatgaca aactgctgtt
      781 gatggttgac gctctgggca atccccttgc aactgatcta acctctaaaa tgtttgccca
      841 ctatcgagat aaacgcctga caggtgagat ctacttcagc gagaaatgga agaaaggagc
      901 aagcccggtc accattaacc tggagcaaag ctatctaagt agtgttttta gcgaactatc
      961 ccgcctgggc gaatggtcgt atccgaaccc actggagaac atgcgaaaat tcaccatcgc
     1021 agaaaaagag atggcatggc ttacccatga gcagattgtt gaactgctgg ctgattgcaa
     1081 acgtcaggac ccaattctgg cactggtagt caagatatgc ctaagcacag gcgcacgctg
     1141 gcgagaagca ataaatctta cccgctcgca agtgaccaaa taccgaatta cctttgtaag
     1201 aacgaagggg aagaaaaaca gaagcatccc tatcagtaaa gagctttacg aagagatcat
     1261 ggcgcttgat gggttcaatt tctttacaga ctgctatttt caatttttat ccgtgatgga
     1321 aaaaacgtct atcgtgctcc ctcgcggtca actgacacac gttctgcgcc atacgtttgc
     1381 ggcgcacttc atgatgtcgg gtggaaatat ccttgctttg caaaaaatcc tcggacatca
     1441 cgatataaaa atgactatgc gctatgccca tttagcaccc gatcatcttg agacggctct
     1501 gagattcaac cccctcgcaa cgctagaagt gtgatctact ggtgcaaaaa taacatcatc
     1561 ccccactagc tgagttaatt gcgtcagagt aaacttttat cagtat
//
LOCUS       DAPHDW010000302         1565 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.309, whole genome shotgun sequence.
ACCESSION   DAPHDW010000302 DAPHDW010000000
VERSION     DAPHDW010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.309"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..136)
                     /locus_tag="RH057_004547"
     CDS             complement(<1..136)
                     /locus_tag="RH057_004547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130767.1"
                     /translation="MGNEKSLAHTRWNCKYHIVFAPKYRRQVFYREKRRAIGSILRKL
                     C"
     gene            complement(331..1308)
                     /gene="wzzB"
                     /locus_tag="RH057_004548"
     CDS             complement(331..1308)
                     /gene="wzzB"
                     /locus_tag="RH057_004548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HDV3130768.1"
                     /translation="MRVENNNVSGQNLEPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLVVAKEKWTSTAIVTQPDVGQIAGYNNAINVIYDSAAPKVSELQSILIGRFSTT
                     FSALAETLDNQEEPEKLTIEPTVKNQSLPLAVSYVGQSPEAAQKQLAQYIQQVDDQVN
                     EELEKDLKDNIALRMKNLQDSLKTQEVVAQEQKELRIRQIQEALQYANQAQVTKPQIQ
                     QTQDVTQDTMFLLGSDALESMVKHEASRPLVFSSTYYQTRQNLLDIESLKVDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
BASE COUNT          389 a          384 c          327 g          465 t
ORIGIN      
        1 cacacagctt tctcaaaata ctgcctattg ctctacgctt ctctctgtag aacacctgtc
       61 ttcggtattt tggcgcaaaa actatgtgat atttacagtt ccatcgggtg tgcgctaagc
      121 tcttttcgtt ccccattggg accccctttt gatttcttgt ttgacacttg cagttgccag
      181 accgcaaggt gttttaacaa atcaaaaggg gttttaataa ctggctcaaa gctgaaagct
      241 ttccggaacc cccagcctag ctgggggttt tctgtgcaca aaaaacgggc acggtgtgac
      301 caccctgtcc gctttcttta agctcaaaaa tcacttcgcg ttgtaattac gcagcgcgtt
      361 acgccccagt acaatacccg cgccaaccat gccgcccagc agcaccgcca gaatcaaggt
      421 gattgccttc ttcgggctat cacgacgaat gggtaacgta ggtttcatca catagcggta
      481 agcgtgaata tcaagatcat caacctttaa gctttcgata tccagcagat tttgacgagt
      541 ctgatagtag gttgacgaga acaccaacgg acggctcgct tcgtgcttaa ccatcgactc
      601 cagtgcatca ctccccaaaa ggaacatggt atcctgggtc acgtcctgtg tctgctgaat
      661 ctgcggcttt gtcacctgtg cctgattcgc atactgcaac gcttcctgaa tttgacgaat
      721 acgcagctct ttttgctcct gagcgaccac ttcctgggtc ttcagagaat cctgcaagtt
      781 tttcatacgc agcgcgatgt tgtctttcag atctttttca agttcttcat ttacctggtc
      841 gtcaacttgc tggatatatt gagccagctg tttctgcgca gcttcaggcg attgcccaac
      901 ataagacacc gctaaaggta atgactgatt tttaacagta ggttcaatgg tcagtttttc
      961 aggttcttcc tgattatcca gcgtttctgc taatgcagaa aatgtcgtgc tgaagcgacc
     1021 aattaaaatc gactggagct cagacacttt cggcgcggct gaatcataaa tcacatttat
     1081 ggcattgtta taaccagcga tttgccccac atcaggttga gtaacgatag ctgtggaagt
     1141 ccatttttct ttggcaacga ccagatatcc tattgccagg gcaatagcga caatgacaga
     1201 aatgatgatt gtcatcttgc cacgccatag ttgcaccagt aaatcaatca aatcaatctg
     1261 ttccggctca aggttttgcc cagaaacatt attattttct actctcatca ttaccctaac
     1321 ggacgttata attttaggtc tgcatagtgt agtgataatc ttagtgaata ctattgaagt
     1381 ggtcaacaaa aactggccac cgagttaggg aaggtgcgaa taagcgggga aattcttctc
     1441 ggctgactca gtcatttcat ttcttcatgt ttgagccgat tttttctccc gtaaatgcct
     1501 tgaatcagcc tatttagacc gtttcttcgc catttaaggc gttatcccca gtttttagtg
     1561 agatc
//
LOCUS       DAPHDW010000303         1545 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.310, whole genome shotgun sequence.
ACCESSION   DAPHDW010000303 DAPHDW010000000
VERSION     DAPHDW010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1545)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1545)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1545
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.310"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..71)
                     /locus_tag="RH057_004549"
     CDS             complement(<1..71)
                     /locus_tag="RH057_004549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HDV3130769.1"
                     /translation="MNLALRKIIYDPISYIHPQRVSL"
     gene            complement(87..821)
                     /locus_tag="RH057_004550"
     CDS             complement(87..821)
                     /locus_tag="RH057_004550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscJ/YscJ/HrcJ family type III secretion inner
                     membrane ring protein"
                     /protein_id="HDV3130770.1"
                     /translation="MKYISLLLFILLLCGCKQQELLNHLDQQQANDVLAVLQRHNINA
                     EKKDQGKTGFSIFVEPTDFASAVDWLKIYNLPGKPDIQISQMFPADALVSSPRAEKAR
                     LYSAIEQRLEQSLKIMDGIISSRVHVSYDVDNGDSGKTALPIHISVLAVYEKDINPEI
                     KINDIKRFIVNSSASVQYENISVVLSKRRDIIEQAPTYEISEPVFAYDKAMPVSILLA
                     LISVATCWLLWKYRAILTNLVRLKIK"
     gene            complement(862..1077)
                     /gene="sctI"
                     /locus_tag="RH057_004551"
     CDS             complement(862..1077)
                     /gene="sctI"
                     /locus_tag="RH057_004551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000566300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod subunit
                     SctI"
                     /protein_id="HDV3130771.1"
                     /translation="MIDLNDRVLNLDNPDDKMISAFANYAVQTENWQQNALQALRSDK
                     EGLTPEKLLVLQDHVLNYNVEVSLVEH"
     gene            complement(1169..1409)
                     /locus_tag="RH057_004552"
                     /pseudo
     CDS             complement(1169..1409)
                     /locus_tag="RH057_004552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311745.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle complex
                     protein"
     gene            complement(1423..>1545)
                     /locus_tag="RH057_004553"
     CDS             complement(1423..>1545)
                     /locus_tag="RH057_004553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001365813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgH/EprH family type III secretion apparatus
                     protein"
                     /protein_id="HDV3130772.1"
                     /translation="GKQYVRFNVNLKNQDTNNSSFSYGDNRFEKSQGSNWTFQE"
BASE COUNT          459 a          311 c          257 g          518 t
ORIGIN      
        1 ttcaagctta ctcgttgagg atgaatataa ctaattggat catatataat ctttcttagg
       61 gcaagattca taacgctctc atatgtctac ttaattttca acctgactaa attagttaga
      121 atggccctat acttccataa cagccagcaa gtcgctacgg atattaatgc aagtaagata
      181 gaaaccggca tagccttatc ataagcaaaa acaggttcgc taatttcata tgttggtgct
      241 tgctcaataa tgtctcttcg ttttgacaat acaacagaaa tattttcata ttgtacgctt
      301 gcagagctat taacaataaa tctcttgata tcatttattt ttatttctgg gttgatatct
      361 ttttcatata ctgcaagtac agaaatatga attggtaaag cagttttacc actatcgcca
      421 ttatcaacat cgtaactaac atgtactctc gaagaaataa tgccatccat aattttgaga
      481 gattgctcta accgctgctc aatagcagaa tatagccttg ctttttccgc tcgtggagat
      541 gaaacgagtg catctgcagg gaacatctgc gatatttgaa tatcaggctt acccggtaga
      601 ttgtagattt ttagccaatc caccgcagaa gcaaaatccg ttggttcgac aaatattgaa
      661 aatcctgttt tgccttgatc cttcttttca gcattaatat tatgtctttg taaaacagca
      721 aggacatcat tagcttgctg ttgatcaaga tggttcaata attcctgctg cttgcagccg
      781 cacaacagca ggataaacaa taataatgat atatatttca tgagcgggtc aacgtctcaa
      841 ctgcagcgac aatttttctt gctaatgttc caccaatgaa acctcgacat tataattaag
      901 cacatgatcc tgcaagacta ataatttttc aggagttaac ccctctttat cagaccttaa
      961 tgcttgtaac gcattttgct gccaattttc agtttgtaca gcatagttgg caaaagcgga
     1021 gatcatttta tcatccgggt tgtcaagatt gagaacccgg tcatttaaat caatcacttt
     1081 cgcttgctgt atttcatgag cagttgatta ctcagctcta tcaataggtg gcatattgct
     1141 tactgacata ataaaatcct tatccagttt agcggaagtt ttgtatgatt gctgaatcaa
     1201 gatctttata cgcttttaca acgttagatt gcgcatttcg atataatgta tactcagcta
     1261 atgcactttg atattcggca agaaatttcg tgtcggaggg attggttggc taaatcctcc
     1321 aacgcttttt caacttgctg gttcagatca tcaacgccct gatcaaattg attgctgata
     1381 tccataatat aaccattcca atctgccata agtcctcctc aattattcct gaaatgtcca
     1441 gttgctacct tgagacttct cgaatcggtt atctccgtag ctaaatgaac tattatttgt
     1501 gtcctgattt ttcaaattca cattaaatct tacatattgt ttacc
//
LOCUS       DAPHDW010000304         1540 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.311, whole genome shotgun sequence.
ACCESSION   DAPHDW010000304 DAPHDW010000000
VERSION     DAPHDW010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1540
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.311"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            480..707
                     /locus_tag="RH057_004554"
     CDS             480..707
                     /locus_tag="RH057_004554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130773.1"
                     /translation="MQAAPQREETEWRVQSKRELMPAYRGEAGQQVNIKIMEYSERNV
                     RQLASNEQEEYIPRKINVGVINTPTLIRSDY"
     gene            complement(742..882)
                     /gene="ykgO"
                     /locus_tag="RH057_004555"
     CDS             complement(742..882)
                     /gene="ykgO"
                     /locus_tag="RH057_004555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HDV3130774.1"
                     /translation="MKVLNSLRTAKERHPDCQIVKRKGRLYVICKSNPRFKAVQGRKK
                     KR"
     gene            complement(882..1145)
                     /locus_tag="RH057_004556"
     CDS             complement(882..1145)
                     /locus_tag="RH057_004556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HDV3130775.1"
                     /translation="MKPNIHPEYRTVVFHDTSVDEYFKIGSTIKTDREIELDGVTYPY
                     VTIDVSSKSHPFYTGKLRTVASEGNVARFTQRFGRFVSTKKGS"
BASE COUNT          454 a          294 c          349 g          443 t
ORIGIN      
        1 tatattggtc aggaactttg ccctgaagtg ttaggaatag tcttaatgcg ctgtgtgatg
       61 gactagtaaa aaaacaagtt ttaagggtaa gggatagaat ataaaactat aatgagctgt
      121 cattgaagat gatttgccaa tcagtaagaa attttaatgg attaacatgc cgcacaaaat
      181 ggttataggt gttcattaca ataagtgatg gaaaattgaa cgggcgtatg tacgtcatct
      241 gttcggtaag cccccaagag ccagattaag tgaagttaaa cgaaggctgc cagctgtcac
      301 aggaacgatg gcctctttgt cagggcactg agtgcagtta tctgtgtgcg ttactctaac
      361 tggcactcga acgtcggcga cggggttacc ggtggatcag actgttcctg tggcgtaaac
      421 gtttttatgt tagtaccaac agatttacca catttatcat cttaacagtt tgagtgaaaa
      481 tgcaggctgc gccgcaaagg gaggagactg agtggcgtgt tcagtctaaa agagagttga
      541 tgccagcata caggggcgaa gctggtcaac aagtcaacat taagataatg gaatattcgg
      601 agagaaacgt caggcagttg gcctcaaatg aacaggagga atatatcccc aggaaaatta
      661 atgttggtgt tataaacaca ccaacattaa tccgatctga ttattaatca gacaaatatc
      721 gttaatccgt cgaattttga atcaacgttt tttcttacga ccctgaacgg ccttaaaacg
      781 tggattagat ttacaaatca catatagccg tccttttcgc ttcacaatct gacagtctgg
      841 atggcgttct tttgcggtac gcagagagtt aagaactttc atcacgaccc ctttttcgtg
      901 ctaacaaaac gaccaaaacg ttgggtgaat cgtgcaacat ttccttctga tgccactgtt
      961 ctcagcttcc ctgtatagaa cgggtgcgat ttagaagaga catcaattgt cacgtatgga
     1021 tacgttacgc catccagctc aatctcacgg tctgttttga tagtcgagcc gattttaaag
     1081 tactcatcaa cactggtgtc gtggaacacc acagtacgat actcaggatg gatattgggc
     1141 ttcatcattt ttacctgtta tgttataaca taaccataaa gatacatgct catccggatc
     1201 atttcaacac ccaatccctg tgactctttg tggatgtgga attttcgcaa cggggaactc
     1261 tggcattgtt agcagccatt ggtgacgctc ccataaacgc ctggatgagt gattgatgat
     1321 ggttttgctg agagtatgga tttttcgaca atggaggtta tctccacttt actggccgat
     1381 gatttatcgc agtatgattg attacctgac agcgtgtatt cctctcccac ggatataata
     1441 attacttaca aaaagggaga ggatgcatat tttaaatatc actgaagtga acagtttatt
     1501 gccgttatta atagaaatgg agaaataaat aggcgtattc
//
LOCUS       DAPHDW010000305         1526 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.312, whole genome shotgun sequence.
ACCESSION   DAPHDW010000305 DAPHDW010000000
VERSION     DAPHDW010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.312"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..536)
                     /locus_tag="RH057_004557"
     CDS             complement(<1..536)
                     /locus_tag="RH057_004557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1705 domain-containing protein"
                     /protein_id="HDV3130776.1"
                     /translation="MNIRKLFCPGNTPRIILFLFFFVVSVITTIACGYTEKNTTGNVL
                     LLFFLLLLAHRNTLTSITALLFLFCCALYAPAGMTYGKINNSFIVALLQTTADEAAEF
                     TGMIPVYHFLVSVTVLVFMVIFWQTHHRGHRNWLALLLFVLCSVNSWPLRMVKGTVVG
                     TTDTLREMQHYKQLSQHGA"
     gene            complement(718..1446)
                     /locus_tag="RH057_004558"
     CDS             complement(718..1446)
                     /locus_tag="RH057_004558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001586155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HDV3130777.1"
                     /translation="MKKVFAVSVVGAACVFAVNAGAEEGKSGFYLTGKAGASVVSLSH
                     QRFLSGNEEETSKYKGGDDHDTVFSGGIAAGYDFYPQFSIPVRTELEFYARGNADTKY
                     NLYKDDWSRGDLKNEVSVNTLMLNAYYDFRNDSAFTPWVSAGIGYARIHQKTTGISTW
                     DYGYGYSGRESLSRSGSADNFAWSLGAGIRYDVTPDIALDLSYRYLDAGDSSVSYKDE
                     WGDKYKSEVDVKSHDIMLGVTYNF"
BASE COUNT          458 a          373 c          302 g          393 t
ORIGIN      
        1 gccccgtgct gactcagttg tttataatgc tgcatttcac gcaatgtgtc agttgtcccc
       61 acaactgttc ctttaaccat ccgcaacggc cagctgttta ctgagcataa tacgaacagc
      121 agcagtgcca gccagttacg gtgaccgcgg tggtgtgtct gccagaaaat caccatgaat
      181 accagaaccg tgacactaac cagaaaatga taaacaggaa tcatcccggt aaactctgca
      241 gcctcatcag ccgtggtctg cagcaacgcg acaataaaac tgttgttgat tttaccgtac
      301 gtcatacccg caggcgcata cagtgcacaa cagaacagaa ataacagcgc tgtaatggat
      361 gtgagggtat ttctgtgtgc aagaagcaga agaaagaaca gcagcaacac attcccggtg
      421 gtattcttct cagtgtatcc gcatgcaatt gtggttatga cagaaacaac aaaaaagaat
      481 aaaaacaata taatccgggg agtgttgccc ggacaaaaca gttttcttat attcatcgga
      541 gtatatcgac aacattatta tgaagagaac aggataataa aaatcagaaa ttatctgtaa
      601 aacagataac agacaaccct gcagcaatag actgcagggt gtttctgttt aattgcagaa
      661 gatatacaat tatattaatt acagaatatt cgtgattatc tctcgggaac agtgttgtca
      721 gaagttataa gtcacaccaa gcatgatgtc atgactttta acatcaacct ctgatttata
      781 tttatcgccc cactcgtcct tgtaactcac actgctgtca cccgcatcaa gatagcgata
      841 gctgaggtcc agagcgatat ccggagtaac gtcatagcgg atacccgcgc caaggctcca
      901 tgcgaagttg tcagcagagc ctgaacgtga caacgattca cgaccactgt atccgtaccc
      961 gtaatcccag gtactgatac cggttgtttt ctggtgaatt ctggcgtagc caatccctgc
     1021 agatacccat ggtgtgaatg cgctgtcatt ccggaagtca tagtacgcat tcagcatcag
     1081 agtgttgact gacacctcat tcttcaggtc accacgcgac cagtcgtctt tataaagatt
     1141 atatttcgtg tcagcatttc cacgagcata aaactccagt tctgtacgaa ccggaatact
     1201 gaactgtgga taaaaatcat aaccggctgc gataccgcca ctgaataccg tgtcatggtc
     1261 atccccacct ttatactttg acgtttcttc ttcatttcct gacaggaaac gctggtgtga
     1321 aagtgacacc acagaggcac cggctttacc ggtcagataa aaaccgcttt taccttcctc
     1381 agcacctgca tttactgcaa atacacaggc tgcgcccacc actgaaacag caaaaacctt
     1441 tttcatttca attatctccg ttattgtatc atcactttaa tatatatttt taatatttaa
     1501 aaaccggact aaaggcagcc tccccc
//
LOCUS       DAPHDW010000306         1513 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.313, whole genome shotgun sequence.
ACCESSION   DAPHDW010000306 DAPHDW010000000
VERSION     DAPHDW010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1513
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.313"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..892
                     /locus_tag="RH057_004559"
     CDS             <1..892
                     /locus_tag="RH057_004559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HDV3130778.1"
                     /translation="PYTVRKRNVVNRATKHFRLTNIANYLSWLSQILKQQQGSINEFV
                     DKIRERRPKLRRRSRQDQPEKSLDGNQINHLLEVIKCGAAFNPFGENLQIRNRLIILV
                     LYSLGIRSGELLNIRISDINFSDSSLAIRRRADDKNDPRVKQPLVKTMERKLPLSSAL
                     AKELHNYITSVRRKFKNARKHEYLFVTHKSGPTQGMPLSSMAYHKVIDSIRNVMPELC
                     NLTGHKLRHTWNYEFSKKLDEMPERVSEAEQEKMRSNLMGWTPGSGTAQIYNKRFIQD
                     KSHKVAIKLQEDINKKGEVE"
     gene            889..>1513
                     /locus_tag="RH057_004560"
     CDS             889..>1513
                     /locus_tag="RH057_004560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130779.1"
                     /translation="MNSVHKVLMSKDLIPAKMDGYYFSPQEDVWVLDRNNQINKKAVT
                     DVLNDNLKEGYLNTIALFARDYSASFASKIHGAFLAFIKEEQCVYVSKASVLNFRSKK
                     HVKDELLFRLRIFITKWYSLGYNGIASEAVQIMKTWKLVNGKPGDVVKRKEPIQGPLT
                     DIELQEFNEGAIRAYEKKEISLFMLTMALIISHTGRRPLQVTQMKITD"
BASE COUNT          551 a          249 c          307 g          406 t
ORIGIN      
        1 tccatatact gtcaggaaga ggaatgttgt aaaccgtgca accaaacact tcagattaac
       61 caatatagca aactatttga gttggttaag tcagatactt aaacagcaac aaggaagtat
      121 aaatgaattc gtcgataaga ttagagagag aagaccaaaa ctaagaaggc gcagccgtca
      181 agaccaacca gaaaaatctc ttgatggaaa ccaaattaat catttattag aagtcataaa
      241 atgtggagcg gcatttaatc cattcggtga aaatcttcag ataagaaacc gacttattat
      301 tctggtgctt tattctttgg gaataaggtc gggagagctt cttaatattc gtatatcgga
      361 tattaacttt agtgattcca gtctggctat ccgtcgtcgg gctgatgata agaacgatcc
      421 acgagttaaa caaccattgg ttaagacgat ggaaagaaaa ctacctctat cttcggcatt
      481 ggcaaaagaa ttgcacaatt acattaccag tgtccgtagg aagtttaaga atgccagaaa
      541 gcatgagtac cttttcgtaa cccataagtc tggcccaaca caaggtatgc ctttatcgag
      601 catggcttac cataaagtta tcgatagcat cagaaatgtc atgcctgagc tttgcaatct
      661 tacggggcac aaactcagac atacctggaa ctatgaattc tctaaaaaat tagatgagat
      721 gcctgagcga gtaagtgagg ctgaacaaga aaaaatgcgt tctaatctta tgggatggac
      781 tcccggttct ggaactgcac agatatataa caaaagattt attcaggata aatcacataa
      841 agtcgcaata aaactacagg aagatattaa taaaaaaggg gaggttgaat gaattcagta
      901 cataaagtgt taatgagtaa agatcttatt ccagctaaaa tggatgggta ttatttttca
      961 ccacaagaag atgtatgggt acttgacaga aacaatcaga taaataaaaa agcagttact
     1021 gatgttctta acgacaattt aaaagaagga tatctaaata caattgcact atttgcgagg
     1081 gattatagcg caagttttgc ctccaagata catggagcat ttcttgcttt tataaaggaa
     1141 gagcaatgcg tatatgtcag taaagcttca gttttaaatt tcagaagcaa aaagcatgtt
     1201 aaggatgaat tattatttcg tctgaggata tttatcacta aatggtactc tcttggctac
     1261 aatggaatag cttcggaagc tgtacagata atgaaaacat ggaaacttgt taacggaaag
     1321 ccaggtgatg tcgttaaaag aaaagaaccc atacaaggtc ctttgactga tattgaattg
     1381 caagaattta acgagggagc aataagagcg tacgagaaaa aagaaatatc tctttttatg
     1441 ttaactatgg cacttattat cagccacaca ggtcgtagac ccttgcaagt aactcagatg
     1501 aagataacag ata
//
LOCUS       DAPHDW010000307         1485 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.314, whole genome shotgun sequence.
ACCESSION   DAPHDW010000307 DAPHDW010000000
VERSION     DAPHDW010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1485
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.314"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..211)
                     /locus_tag="RH057_004561"
     CDS             complement(<1..211)
                     /locus_tag="RH057_004561"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130780.1"
                     /translation="MTRHEIQNRCPELLITNYSMLEYMLMRPIERNIFEQTKEWLKAD
                     EMNELILVLDEAHMYRGAGGAEIALL"
     gene            332..487
                     /locus_tag="RH057_004562"
     CDS             332..487
                     /locus_tag="RH057_004562"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130781.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQQLVAASCDPGISISKLALENGI
                     NANLLFK"
     gene            709..1056
                     /gene="tnpB"
                     /locus_tag="RH057_004563"
     CDS             709..1056
                     /gene="tnpB"
                     /locus_tag="RH057_004563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019104956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3130782.1"
                     /translation="MIPLPSGTKIWLVAGITDMRNGFNGLAAKVQTALKDDPMSGHVF
                     IFRGRSGSQVKLLWSTGDGLCLLTKRLERGRFAWPSARDGKVFLTQAQLAMLLEGIDW
                     RQPKRLLTSLTML"
     gene            1106..>1485
                     /locus_tag="RH057_004564"
     CDS             1106..>1485
                     /locus_tag="RH057_004564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV3130783.1"
                     /translation="MNDISSDDIFLLKQRLAEQEALIHALQEKLSNREREIDHLQAQL
                     DKLRRMNFGSRSEKVSRRIAQMEADLNRLQKESDTLTGRVYDPAVQRPLRQTRTRKPF
                     PESLPRDEKRLLPAAPCCPNCGGSL"
BASE COUNT          364 a          411 c          378 g          332 t
ORIGIN      
        1 ttaaaagggc tatctctgct ccccctgctc ctctatacat atgcgcttca tcaagcacta
       61 agataagctc attcatctca tcagctttga gccattcctt agtctgctca aaaatattac
      121 gctcgatagg tcgcatcagc atatactcaa gcatggagta gttcgttatc agtaattctg
      181 gacagcgatt ctgtatttca tgccgggtca ttagctcacg atcctcaggc tgggtaattg
      241 taagcgtaca gcctgaaccg tctggtcaga atctgacgaa ttagacaaag tggtgtccac
      301 caaataagta gtgggaacca aagtgtcaga tatgcagaaa aatgtgactc ccggcaggcg
      361 aaaaggctgc cctaattatc ctcccgaatt taaacagcag ctcgttgctg cctcctgtga
      421 ccccgggata tccatctcaa aacttgctct tgaaaatggc attaacgcca atctgttgtt
      481 caaatgacga caacaatggc gcgagggaaa gctgctatta ccttcttcag agagccccca
      541 gctacttcct gtgactcttg atgcagttgc cgaacagcca gaatcgctcg cagaggaccc
      601 ggaaaccctc agtatcagct gtgaggtaac gttccggcac gggacgctcc gcttcaatgg
      661 caatgtcagc gaaaagctcc tgactctgct gatacaggaa ctgaagcgat gatcccgtta
      721 ccttccggga ccaaaatttg gctggttgcc ggtatcaccg atatgagaaa tggcttcaac
      781 ggcctggctg cgaaagtaca gacggcgctg aaagacgatc ccatgtccgg ccatgttttc
      841 attttccggg gccgcagcgg tagtcaggtt aaactgctgt ggtccaccgg tgacggactg
      901 tgcctcctga ccaaacggct ggagcgtggg cgcttcgcct ggccgtcagc ccgtgatggc
      961 aaagtgttcc ttacgcaggc gcagctggcg atgctgctgg aaggtatcga ctggcgacag
     1021 cctaagcggc tgctgacctc cctgaccatg ctgtaaatct ctttatcctg gttgtcacag
     1081 aataagcccg gtaaaatacg ggcttatgaa cgacatctct tctgacgaca tcttcctgct
     1141 gaaacagcgc ctggccgaac aggaagcgct gatccacgcc ctgcaggaaa agctgagcaa
     1201 ccgggagcgc gaaatagacc atctgcaggc gcagctggat aaactccgcc ggatgaactt
     1261 cggcagtcgt tccgaaaaag tctcccgccg tatcgcacaa atggaagccg atctgaaccg
     1321 gcttcagaaa gagagcgata cgctgactgg tagggtgtat gacccggcag tacagcgtcc
     1381 gttgcgtcag acccgcaccc gtaagccgtt ccctgaatca ctaccccgtg acgaaaagcg
     1441 actgttgcct gcggcgccgt gctgcccgaa ctgcggcggt tcact
//
LOCUS       DAPHDW010000308         1461 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.315, whole genome shotgun sequence.
ACCESSION   DAPHDW010000308 DAPHDW010000000
VERSION     DAPHDW010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.315"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1297
                     /locus_tag="RH057_004565"
     CDS             <1..1297
                     /locus_tag="RH057_004565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HDV3130784.1"
                     /translation="LVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYF
                     IYDRRQFSNCILSLAFLSCLIYFVKTVIIIQQIIEGRLTSSVVQNDIAIYYLFRQMSL
                     CILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAE
                     LTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLL
                     FMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFF
                     NELTVQSASAKKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLL
                     ARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQE
                     NALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP"
     gene            complement(1338..>1461)
                     /locus_tag="RH057_004566"
     CDS             complement(1338..>1461)
                     /locus_tag="RH057_004566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV3130785.1"
                     /translation="SREIMRATVFNYIECDYNRWRRHSWCGGLSPEQFENKNLA"
BASE COUNT          407 a          259 c          324 g          471 t
ORIGIN      
        1 tttggttttg gtgaatagtt ggtttaatca gccaggcgtt gaggaagttg ttccacgatc
       61 aacctatctg atggtaatga ttgctttatt ttttatcgat actgttgcat ttatttttat
      121 gcagttgtat ttcatttatg accgtaggca attttcaaac tgtatactta gtttggcttt
      181 tctgagttgt ttgatttact ttgttaaaac cgtcattatc attcagcaaa ttattgaggg
      241 gcgtttgaca agcagtgttg tccaaaatga cattgcaatc tattatttgt ttcgtcagat
      301 gagtttgtgc atattaatat ttctggcatt ggtgaataaa gttagtgaaa acacaaaaca
      361 gcgcaattta ttttccaaaa aaatgacttt gtgcataagt ttgttttttg tttttggggg
      421 gccgatagtt gctcacatac tttctagtca ttatgagagc tacaatttac acatagccga
      481 gttaaccaat gaaaatggtc aggtggtttg gaaagcctca tatgttacca taatgatttt
      541 catgtggtta accttgcttt cggttaatct atactttaat ggtttacgct atgatatctg
      601 gaatggagtg acggtgattg ctttttgtgc cgtactgtac aatatctctc tgttatttat
      661 gagtaggtat agcgtgtcca cttggtatat tagtcgcact attgaagtag tcagtaagtt
      721 aaccgttatg gtaatattta tgtgccatat attcagtgcg ctacgagtaa caaagaacat
      781 tgcacatcgc gatcccttaa ccaatatatt taacagaaat tattttttta atgaactgac
      841 agttcaatca gcatcagcca aaaaaacgcc ttattgcgtc atgattatgg atatcgacca
      901 cttcaaaaaa gtcaacgaca cctgggggca tccggttggc gatcaggtga taaaaacagt
      961 ggtgaatatc attgggaaaa gcatacgacc agatgacctt ttagcgcgcg tcggcggcga
     1021 agagtttggc gtcttgctga cggacatcga tactgaacgt gcgaaagctt tagcggaaag
     1081 gattcgggaa aatgttgagc gtttaactgg cgataatcct gaatacgcta taccacaaaa
     1141 agtgacgatt agtattggcg ctgttgtgac tcaggaaaat gcgttaaacc caaatgagat
     1201 ctatcgactg gctgataacg cactttacga ggcgaaagag accgggcgta ataaggtggt
     1261 tgtgagggat gtggtgaatt tttgtgagtc accataaagc ggcattttga tcctacccac
     1321 gtaatatgga cacaggccta agcgaggttc ttgttttcaa attgttccgg actgaggccg
     1381 ccacaccaac tgtgccgccg ccaccgattg taatcacatt cgatataatt aaacaccgtt
     1441 gcccgcatta tttcccggct g
//
LOCUS       DAPHDW010000309         1454 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.316, whole genome shotgun sequence.
ACCESSION   DAPHDW010000309 DAPHDW010000000
VERSION     DAPHDW010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1454
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.316"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            11..1192
                     /locus_tag="RH057_004567"
     CDS             11..1192
                     /locus_tag="RH057_004567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HDV3130786.1"
                     /translation="MEDTPQKTLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTNLSLS
                     VWAALLMLGSMLFLPSSPWWGKQISLTGSKTVVLWALGGYGVSFTLLGLGSVLMATGA
                     VTTAVGLGILIIARIVYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSCGRLF
                     GPLCAAAMLVIHPLAPVWMLMAAPVWAVVMLLRLPGTPPQPTPERKSVSLKRDCLPYL
                     LCAMLLAAAMSMMQLGLSPALTRQFDTDTTTISQQVAWLLGLSAIAALIAQFVVLRPQ
                     RLTPVALLLSAGVLMSSGLAIMLTEQLWLFYLGCAVLSFGAALATPAYQLLLNDKLAD
                     GAGAGWLAASHTLGYGLCALLVPLASKTGVSIALIVTALFAAVLFTIVSACIWHYRTI
                     K"
BASE COUNT          285 a          373 c          424 g          372 t
ORIGIN      
        1 gtcatctaag atcgaagata cgccccaaaa aaccctgtcc tgctggccac tggcgttcag
       61 tgccggtctt ctcggtatcg gacagaacgg tctgctggtt gtactccctg ttctggtcat
      121 acagacaaat ctgagtctgt ctgtatgggc tgccctgctg atgctgggct caatgctgtt
      181 tctgccatct tccccatggt ggggaaagca aatttccctt actggcagta agactgtggt
      241 gctgtgggct ctgggaggat atggcgtaag ctttaccctg cttgggctgg gaagcgtgct
      301 gatggctacc ggtgccgtaa caacagcggt ggggttggga atattaatca tcgcccggat
      361 cgtctacggt ctgaccgtgt cagcaatggt gccagcctgt caggtctggg cattgcagag
      421 agcgggagaa gggaatcgca tggccgctct ggcaaccatc agctccggcc tgagctgcgg
      481 caggctattc gggccgctgt gcgcggcggc aatgttggtc attcaccctc tggcgccagt
      541 gtggatgctg atggcagctc cggtgtgggc tgtggtgatg cttctgcggt tgcccggcac
      601 accaccacag cccacaccgg agcgcaagag cgtcagcctg aagcgggatt gcctgcctta
      661 tctgctttgc gcaatgttac tggctgcggc aatgagcatg atgcagcttg gactttcgcc
      721 agcccttact cgccagttcg atactgatac caccactatt agccaacagg tggcgtggtt
      781 gttggggctg tccgcaatag ctgcgcttat cgcgcagttc gtggtactcc gtccacagcg
      841 cctgactcca gtggctctgc tcctgagtgc cggggtgttg atgagtagtg gtctggctat
      901 catgctcact gaacagctat ggttgtttta cctgggctgt gcagtgctgt catttggggc
      961 cgctctggca acacccgctt atcaactttt actgaatgat aagctggccg acggcgcagg
     1021 cgcgggctgg cttgcggcca gtcacacact tggctatggg ctgtgcgcat tgttggtgcc
     1081 actggcgtcg aaaacaggtg tctcaatcgc gctgattgtg acggcattat ttgccgctgt
     1141 attatttacc atagtgtctg catgtatttg gcattaccgt acaatcaagt aaggctatga
     1201 tgttgtactc acagccggca taacgtcctg tgcaatttcg ctgtcgaaac cgcctgtgct
     1261 gaccgcccag tgcactcacc tgaagcacat cgcagcatgc caccaggata ccagatcgtc
     1321 ttctgaaatg ggtggataca ttggcaacgt taaccgcaac aatttttcag agagaatttt
     1381 ggggggtgag ggccagtgat gtaaaaaata cgccaagagg ttaattcatt gtttatactg
     1441 atatgtcagg taat
//
LOCUS       DAPHDW010000310         1442 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.317, whole genome shotgun sequence.
ACCESSION   DAPHDW010000310 DAPHDW010000000
VERSION     DAPHDW010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1442
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.317"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     repeat_region   38..1347
                     /inference="COORDINATES: alignment:crt:1.2"
                     /rpt_family="CRISPR"
                     /rpt_type=direct
                     /rpt_unit_range=38..66
                     /rpt_unit_seq="gtgttccccgcgccagcggggataaaccg"
BASE COUNT          325 a          428 c          425 g          264 t
ORIGIN      
        1 aaccgccagg gtgagccagc gccgcaggaa gacacgcgtg ttccccgcgc cagcggggat
       61 aaaccgcgtt cccaacgctg aatattgctg gcatagccgt gttccccgcg ccagcgggga
      121 taaaccgcca atacggagaa ccttcatcgg taatggggtg tgttccccgc gccagcgggg
      181 ataaaccgga gcgtagagat tctcgaaacc gatccggacg gtgttccccg cgccagcggg
      241 gataaaccgg aagttgcgcg cactaagaaa aacaggttcg agtgttcccc gcgccagcgg
      301 ggataaaccg ctgaaatcaa tcgccggtcg tcgccacgtt acgtgttccc cgcgccagcg
      361 gggataaacc gtcatcgaaa accagcggat cgaatgggac gccgtgttcc ccgcgccagc
      421 ggggataaac cgagacggcg cgtaaaactc atacggcgac aaatgtgttc cccgcgccag
      481 cggggataaa ccgctcgcgg gcgtggaatt taacgaacgg ttaccgtgtt ccccgcgcca
      541 gcggggataa accgtcggga cgctgcgcga tctgcatagc aacgcagtgt tccccgcgcc
      601 agcggggata aaccggtatt tttgctgacg gattctcaga gagtttcgtg ttccccgcgc
      661 cagcggggat aaaccgtttc tatctcccag tgggagagag atgacagtgt gttccccgcg
      721 ccagcgggga taaaccggtt ttggcgatat cacctgatgc ctgcaatccg tgttccccgc
      781 gccagcgggg ataaaccggg gtatcgcact gcggcagatg ttccggggcc gtgttccccg
      841 cgccagcggg gataaaccgc atgaatatgg acgatgaaaa aataagagag ggtgttcccc
      901 gcgccagcgg ggataaaccg ttaaaacatt ttccgagctt tgcgatttat atgtgttccc
      961 cgcgccagcg gggataaacc gtaaaaatca tcatggaaaa tcaacccggc gacgtgttcc
     1021 ccgcgccagc ggggataaac cgagcgcgcg cgggctactg cactcggtga taacgtgttc
     1081 cccgcgccag cggggataaa ccggcgatct cgcggaatac accgacgagg cgggcgtgtt
     1141 ccccgcgcca gcggggataa accgtaaggc cgtcgccgga tcagcctggc tatgccgagc
     1201 tccccgcgcc agcggggata aaccgttctt gcgggtgttg caaatattct tcacgtagag
     1261 ctccccgcgc cagcggggat aaaccggacg ccgccgccgc gaagccgttt ccgatgttga
     1321 gctccccgcg ccagcgggga taaaccacca tataacccgt tatctctttc tcaagttttt
     1381 atattagcag tacttgtaat aagcaacata tccacgtaac acctcatgtt caaaatagtt
     1441 ct
//
LOCUS       DAPHDW010000311         1420 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.318, whole genome shotgun sequence.
ACCESSION   DAPHDW010000311 DAPHDW010000000
VERSION     DAPHDW010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1420
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.318"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..902)
                     /locus_tag="RH057_004568"
     CDS             complement(<1..902)
                     /locus_tag="RH057_004568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3130787.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVSDVE
                     RGKLDEIDATGYDIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YETQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPQR
                     AKEARPFRLAVIQLGTYDGTIYNAR"
     gene            complement(1213..1317)
                     /locus_tag="RH057_004569"
     CDS             complement(1213..1317)
                     /locus_tag="RH057_004569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HDV3130788.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
BASE COUNT          421 a          315 c          316 g          368 t
ORIGIN      
        1 cgggcgttat aaatcgtacc gtcgtaagtg cctagctgaa taacggcgag gcggaaagga
       61 cgagcctctt ttgcccgctg cggtgcaact tctgtaatca gctcacgcag gtaactttct
      121 tcaaaacagt gagcgtcaat accaccgata aaaccatacg ggttacgtgc ggtttccaga
      181 taaaccggtg tcgcgccagc ctgtagcaac gcaccgtggt ggttagattt atggttattg
      241 cggtcaaaca acaccagatc gcccggcgtc agcaatgcgt ttaagaccac tttattagaa
      301 gatgaagtac cgtttaaaac gaaataggtt ttatcggcat taaagacttt agccgcatgc
      361 tgttgtgcaa tacatggcgc accttcatga atcagcagat cgcccattgc tacgtcagca
      421 ttacacaagt cagagcggaa gagcatttcg ccaaagtatt ccacgaactg attgcctgcc
      481 ggatggcgac ggaaaaactc acccccctga tgaccagggc agtcaaaagc actattgccc
      541 tgattaacat aatcgaccag tgcgcggaag aagggcgggc gcagttgcgt ttcataatgg
      601 ctggcagcgg tttctaactg acgtccataa aactctttac gtgattcgca atgttcgaag
      661 acaccagaaa tacgtggcag atattctgcg ggtacacgtt cttcattttc cgttgcaata
      721 aaaacaggaa tgtcatagcc agtagcgtct atttcatcga gttttccacg ttcaacatca
      781 ctgactgata aaataatggc ggcaacgtca atataattac tttcgtctat attcacgcat
      841 gcacgatgag tggaaaaaca atccgggcaa gaacgactaa cggcaatttt taattctgac
      901 attttgttca tctctttatt tcaggtaata gcaggtcctc aatttctcaa aggagaaatc
      961 gatcacgccg gaaaaaaagc aaagctccac cgctgataaa taaatcagta gtttgctttt
     1021 tgcagatgca ataacccaga ccagcccggt aactggctat actcgtcata cctcaagttg
     1081 caagtgctgc gactcgaatt atttagagtt taaaagggtt agcagtatat gagcaccgag
     1141 gaatacaggt attaacctgt aatgcttaaa gtcagggaga cctgtacgtt ggggtgccct
     1201 ttagtcagaa gactagcggg cattaaagaa atgaaagtca aaactattac gatgggcaaa
     1261 cttcatgata tgggttgtcc gccttatatg aggcattaag cggttattat tttccattta
     1321 cctttacctc ttgctgtgtc agttgaaggt agagttattc tatccatgaa atggctataa
     1381 tctgtgaaga agatcaacaa aaaacgatca taccccaaat
//
LOCUS       DAPHDW010000312         1378 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.319, whole genome shotgun sequence.
ACCESSION   DAPHDW010000312 DAPHDW010000000
VERSION     DAPHDW010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.319"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            31..106
                     /locus_tag="RH057_004570"
     tRNA            31..106
                     /locus_tag="RH057_004570"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:64..66,aa:Val,seq:tac)
     gene            111..186
                     /locus_tag="RH057_004571"
     tRNA            111..186
                     /locus_tag="RH057_004571"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:144..146,aa:Lys,seq:ttt)
     gene            217..342
                     /locus_tag="RH057_004572"
     ncRNA           217..342
                     /ncRNA_class="other"
                     /locus_tag="RH057_004572"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(451..1335)
                     /gene="xapR"
                     /locus_tag="RH057_004573"
     CDS             complement(451..1335)
                     /gene="xapR"
                     /locus_tag="RH057_004573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator XapR"
                     /protein_id="HDV3130789.1"
                     /translation="MERVYRTDLKLLRYFLAVAEELHFGRAAARLNMSQPPLSIHIKE
                     LENQLGTQLFIRHSRSVVLTHAGKILMEESRRLLVNANNVLARVEQIGRGEAGRIELG
                     VVGTAMWGRMRPVMRRFLRENPNVEVLFREKMPAMQMALLERRELDAGIWRMATEPPT
                     GFTSLRLHESAFLVAMPEEHHLSSFSTVPLEALRDEYFVTMPPVYTDWDFLQRVCQQV
                     GFSPVVIREVNEPQTVLAMVSMGIGITLIADSYAQMNWPGVIFRPLKQRIPADLYIVY
                     ETQQVTPAMVKLLAALTQ"
BASE COUNT          356 a          369 c          320 g          333 t
ORIGIN      
        1 atttcttgta ataatgtgaa gtaccgaagt gggtgattag ctcagctggg agagcacctc
       61 ccttacaagg agggggtcgg cggttcgatc ccgtcatcac ccaccacttc gggtcgttag
      121 ctcagttggt agagcagttg acttttaatc aattggtcgc aggttcgaat cctgcacgac
      181 ccaccaatgt aaaaaagcgc cctaaaggcg cttttttgct atctgcgata ctcaaagatt
      241 cgaacctgca gcaggtttga gttgagcgca gcgaaacaac ggagccgctc gcggcgacgg
      301 cccgaagggc gagcgaagcg agtcatcctg cacgacccac caatgtaaaa aagcgcccta
      361 aaggcgcttt tttgctatct gcgatttgcg aaattgcctg atgcgcttca cttagcagac
      421 gactatttcc ggcaattcct gtctcctcac ctactgtgtc aatgcagcca acagcttaac
      481 catcgcgggc gtcacctgct gtgtttcata aacaatatat aaatctgcag ggatgcgctg
      541 tttgagcgga cggaaaatga cacctggcca gttcatttgt gcgtagctgt ccgctatcaa
      601 tgtgatacca atacccatac tgaccatggc gagtaccgtt tgcggttcat taacttcgcg
      661 aataacaacc ggtgaaaatc ccacctgctg gcaaactcgc tgcaaaaaat cccagtcagt
      721 gtacacgggc ggcattgtaa caaaatactc gtcacgtagc gcttccagcg gaacggtgga
      781 aaatgatgag agatgatgct cttcaggcat cgccaccaga aacgccgatt catgcaaccg
      841 taagctggta aaacctgtcg gtggttctgt cgccattcgc cagatcccgg catcgagttc
      901 acgtcgttcc agcagggcca tttgcatcgc gggcatcttt tcgcgaaaaa gaacttcaac
      961 gttaggattt tccctgagga atcgccgcat aaccgggcgc atccgtcccc acatagccgt
     1021 tcccactacg ccgagttcaa tccgccctgc ttctccccga cctatttgtt caacccgagc
     1081 caatacatta ttagcattca ccagcaatcg acgcgattct tccatcaaga ttttgcccgc
     1141 gtgtgtcagt acgacgctgc gcgaatggcg aataaaaagc tgcgtgccga gttgattttc
     1201 cagctcttta atatgaatac tgagcggagg ctgagacata tttaaacgcg ctgctgcgcg
     1261 gccaaaatgc aactcttccg ctacggcaag aaaataacgg agcaacttaa gatctgttct
     1321 gtatacgcgt tccataatta agtgctccat ataataaata catcgtatta aattatca
//
LOCUS       DAPHDW010000313         1370 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.320, whole genome shotgun sequence.
ACCESSION   DAPHDW010000313 DAPHDW010000000
VERSION     DAPHDW010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.320"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            94..1305
                     /gene="bluF"
                     /locus_tag="RH057_004574"
     CDS             94..1305
                     /gene="bluF"
                     /locus_tag="RH057_004574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="blue light-responsive regulator BluF"
                     /protein_id="HDV3130790.1"
                     /translation="MLTTLIYRSHIRDDEPVKKIEEMVSIANRRNMQSDVTGILLFNG
                     SHFFQLLEGPEEQVKMIYRAICQDPRHYNIVELLCDYAPARRFGKAGMELFDLRLHER
                     DDVLQAVFDKGTSKFQLTYDDRALQFFRTFVLATEQSTYFEIPAEDSWLFIADGSDKE
                     LDSCTLSPTINDHFAFHPIVDPLSRRIIAFEAIVQKNEDSPSAIAVGQRKDGEIYTAD
                     LKSKALAFAMAHALELGDKMISINLLPMTLVNEPDAVSFLLDEIKANALVPEQIIVEF
                     TESEVISRFDEFAEAIKSLKAAGISVAIDHFGAGFAGLLLLSRFQPDRIKISQELITN
                     VHKSGPRQAIIQAIIKCCTSLEIQVSAMGVATPEEWMWLESAGIEMFQGDLFAKAKLN
                     GIPSIAWPEKK"
BASE COUNT          396 a          289 c          293 g          392 t
ORIGIN      
        1 agaaacagtt atgttttggt attaaaagaa agacacaaga agatatgtct gttaccgtct
       61 tactctcgcc tcacccatta ccctggattg ttaatgctta ccacccttat ttatcgtagc
      121 catatacgtg acgacgaacc tgtcaaaaaa atcgaagaaa tggtttcgat agcaaatcgc
      181 aggaacatgc agtctgacgt aacagggatc ttactgttta atggttctca ttttttccag
      241 cttctggaag gtccggaaga acaggttaaa atgatatatc gggctatatg ccaggatcca
      301 cggcactata atattgttga gctgctgtgc gattacgcgc ctgctcgccg ttttggcaaa
      361 gcgggaatgg aattatttga tttgcgcctg cacgagcgag atgacgtttt acaggccgta
      421 ttcgacaaag gcacatcaaa atttcagcta acttatgatg acagagcgct acaatttttt
      481 cgtacttttg tccttgcaac cgaacaatca acctatttcg agatccctgc cgaagactcc
      541 tggcttttta tcgctgacgg atctgataaa gaacttgatt cctgtaccct ttcaccaact
      601 ataaacgacc actttgcctt tcatcctatt gtcgatccct tatcgcggcg gataatcgct
      661 tttgaagcca ttgtgcaaaa aaatgaagat agcccatcag ccatagcggt tgggcagcgt
      721 aaagacgggg aaatctacac agcggatctc aaaagtaagg cgcttgcatt cgcgatggca
      781 cacgcacttg agctcggtga taaaatgatt tcaatcaatc tattacctat gacccttgtt
      841 aacgaacctg acgcagtctc ttttttactt gatgaaataa aggccaatgc tctggtgcct
      901 gaacaaatca tcgttgaatt tactgaaagt gaagttatat ctcggtttga tgagtttgca
      961 gaagcgatta aatcgcttaa ggctgccggt atcagtgtag caattgatca ttttggcgca
     1021 ggttttgccg gtttgttact cctgtcacgc ttccagcctg acagaattaa aatcagtcag
     1081 gaattgatta ccaatgttca taaaagcggg ccacggcagg caattattca agcgatcata
     1141 aaatgctgta catcacttga aattcaagtc agtgctatgg gcgtggcaac accagaagag
     1201 tggatgtggc ttgaatccgc aggaattgag atgtttcagg gagatctgtt tgctaaagct
     1261 aaattgaatg gtatcccttc catagcgtgg ccagagaaaa aataattttc agcacattct
     1321 ttcacatgat ttcagtaaat catcgtgaaa atgaatacgt tacgtttaaa
//
LOCUS       DAPHDW010000314         1351 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.321, whole genome shotgun sequence.
ACCESSION   DAPHDW010000314 DAPHDW010000000
VERSION     DAPHDW010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.321"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..350)
                     /locus_tag="RH057_004575"
     CDS             complement(<1..350)
                     /locus_tag="RH057_004575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HDV3130791.1"
                     /translation="MDQVVVFQKMFEQVRKEQNFSWFYSELKHHRIAHYIYYLATDNI
                     RIITHDDTVLLLRGTRNLLKVSTTKNPAKIKEAALLHICGKSTFREYCSTLAGAGVFR
                     WVTDVNHNKRSYYA"
     gene            complement(571..1302)
                     /gene="bluR"
                     /locus_tag="RH057_004576"
     CDS             complement(571..1302)
                     /gene="bluR"
                     /locus_tag="RH057_004576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor BluR"
                     /protein_id="HDV3130792.1"
                     /translation="MAYYSIGDVAERCGINPVTLRAWQRRYGLLKPQRSEGGHRLFDE
                     EDIQRIEEIKRWISNGVPVGKVKALLETTSQDTEDDWSRLQEEMMSILRMANPAKLRA
                     RIISLGREYPVDQLINHVYLPVRQRLVLDHNTSRIMSSMFDGALIEYAATSLFEMRRK
                     PGKEAILMAWNVEERARLWLEAWRLSLSGWHISVLADPIESPRPELFPTQTLIVWTGM
                     APTRRQNELLQHWGEQGYKVIFHAP"
BASE COUNT          346 a          313 c          305 g          387 t
ORIGIN      
        1 atggcgtagt aactgcgttt gttatgatta acatcagtaa cccaccggaa aacgcccgcg
       61 cctgctagtg ttgaacagta ttcccgaaat gtagattttc cgcaaatatg aagcaatgcg
      121 gcctctttta ttttagcagg gttcttggtc gtactaactt ttaacaggtt cctggttcct
      181 cttaataaca aaaccgtgtc atcgtgagta ataattctga tgttatccgt agccagataa
      241 taaatgtaat gtgcaatacg gtgatgtttt aattctgaat aaaaccagga gaagttttgc
      301 tcttttctca cttgctcaaa catcttttga aaaacaacga cctgatccat caggataata
      361 acctcttgtt agttgtgaga ctgcgtagtg tgcacgatcg gttttaccac ttcaatctgg
      421 tctgtccttt ggctgtgata tgtacagagt gtgatagagg gaatatctga attctcccgg
      481 tgagcatttt gcaacggacc agctccggta caaacgctgt tgtgggttca gattataaca
      541 ttctgtctaa ggggcgggat aaaggtgaaa ttagggggca tgaaagatga ctttataacc
      601 ttgctcaccc caatgttgta aaagttcgtt ttgccttctc gttggtgcca tgcctgtcca
      661 gacaatcaat gtttgcgtcg ggaacagttc cgggcgcggc gattcaatgg gatcagcaag
      721 aacagaaatg tgccatcctg acaatgataa acgccatgct tccagccaca atcgtgctct
      781 ctcttcaaca ttccacgcca tcagaatggc ttctttaccg ggcttacggc gcatttcgaa
      841 aagcgaggtt gctgcgtatt caattaatgc gccgtcaaac atactgctca taatgcggga
      901 ggtgttgtga tcaagcacga gacgctggcg aacaggaagg taaacatgat taatcaattg
      961 atctactggg tactctcgac ccagtgaaat aattctcgcg cgtagtttgg caggattagc
     1021 catgcgaaga attgacatca tctcttcttg caggcggctc cagtcatctt ccgtatcctg
     1081 gctggtggtt tccagtaatg ctttaacttt gcctacaggg acgccattac ttatccaacg
     1141 cttgatctct tcgatgcgtt gtatgtcttc ttcatcaaag agtcggtgtc cgccttcact
     1201 gcgctgtggt tttaacaaac cgtagcggcg ttgccaggcc cggagagtga caggattaat
     1261 cccgcaacgt tcagcaacat caccaatgct gtaataagcc acaattcctc cttgcggtca
     1321 caaatctccg tcgcctgtac acgacccaat a
//
LOCUS       DAPHDW010000315         1339 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.322, whole genome shotgun sequence.
ACCESSION   DAPHDW010000315 DAPHDW010000000
VERSION     DAPHDW010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1339
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.322"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..902)
                     /locus_tag="RH057_004577"
     CDS             complement(<1..902)
                     /locus_tag="RH057_004577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3130793.1"
                     /translation="MSELKIAVSRSCPDCFSTHRACVNIDESNYIDVAAIILSVNDVE
                     RGKLDEIDATGYGIPVFIATENEERVPAEYLPRISGVFEHCESRKEFYGRQLETAASH
                     YEMQLRPPFFRALVDYVNQGNSAFDCPGHQGGEFFRRHPAGNQFVEYFGEMLFRSDLC
                     NADVAMGDLLIHEGAPCIAQQHAAKVFNADKTYFVLNGTSSSNKVVLNALLTPGDLVL
                     FDRNNHKSNHHGALLQAGATPVYLETARNPYGFIGGIDAHCFEESYLRELITEVAPHR
                     AKEARPFRLAVIQLGTYDGTIYNAR"
BASE COUNT          373 a          303 c          312 g          351 t
ORIGIN      
        1 cgggcgttat aaatcgtacc gtcgtaggtg cctaactgaa tcacagcgag gcggaacgga
       61 cgcgcctctt ttgcccggtg cggtgcaact tctgtaatca gttcacgcag ataactttct
      121 tcgaagcagt gcgcatcaat accaccgata aaaccatacg ggttacgtgc ggtttccaga
      181 taaaccggtg ttgcaccagc ctgtagcaac gctccgtggt ggttagattt gtggttattg
      241 cggtcaaaca gcaccagatc acccggtgtc agcagggcgt ttaaaaccac tttgttggag
      301 gaggaggtgc cgtttaaaac aaagtacgtt ttatcggcat taaacacttt agccgcatgt
      361 tgctgtgcaa tgcatggcgc accttcatga atcagtagat cgcccattgc tacgtcggca
      421 ttacacaagt cggagcggaa gagcatttcg ccaaaatatt ccacgaactg attgcctgcg
      481 ggatggcgac ggaaaaactc accgccctga tgacccgggc agtcaaaagc gctattgccc
      541 tgattgacat aatcgaccag tgcgcggaag aagggcgggc gcagttgcat ttcataatgg
      601 ctggcagcgg tttctaactg acgtccgtaa aactctttcc gtgattcgca atgctcgaag
      661 acaccagaaa tacgtggcag atattctgcg ggtacacgtt cttcattttc cgttgcaata
      721 aaaacaggaa tgccgtagcc agtagcgtct atttcatcga gttttccacg ttcaacatca
      781 ttgactgata aaataatggc ggcaacgtca atataattac tttcgtcaat attcacgcat
      841 gcacgatgag tggaaaaaca atccgggcaa gaacgactaa cggcaatttt taattctgac
      901 attttgttca tctctttatt tcaggtaata gcaggtcctc aatttctcaa aggagaaatc
      961 gatcgcgccg gaaaaaagca aaactccaca gctgataaat aaatcagtag tttgcttttt
     1021 gcagatgcaa taacccagac cagcccggta actggcgata ctcatcatac ttcaagttgc
     1081 ctatgctgcg actcgaatta tgtagagttt aaaagggtta gcaatatatg agcaccgagg
     1141 gatacgggta ttaacctgta atgcttaaag tcagtgagac ctgtacgctg gggtgccctt
     1201 tagtcagaag actaacgggc attaaagaaa tgaaagtcaa aactattacg atgggcaaac
     1261 ttcatgatat gtgttacgcg cctgatatgg ggcatttttc ggttattgtt ttccatttca
     1321 tatacctttc tgtatcact
//
LOCUS       DAPHDW010000316         1330 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.323, whole genome shotgun sequence.
ACCESSION   DAPHDW010000316 DAPHDW010000000
VERSION     DAPHDW010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1330
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.323"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            219..>1330
                     /gene="pgaA"
                     /locus_tag="RH057_004578"
                     /gene_synonym="hmsH"
                     /gene_synonym="ycdS"
     CDS             219..>1330
                     /gene="pgaA"
                     /locus_tag="RH057_004578"
                     /gene_synonym="hmsH"
                     /gene_synonym="ycdS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309297.1"
                     /note="outer membrane protein essential for the synthesis
                     of poly-beta-1,6-N-acetyl-D-glucosamine (PGA); adhesin
                     required for biofilm formation; PgaA (HmsH) seems to
                     translocate and/or dock PGA to the cell surface; member of
                     the hmsHFRS (ycdSRQP or pgaABCD in Escherichia coli)
                     operon in Yersinia; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine export
                     porin PgaA"
                     /protein_id="HDV3130794.1"
                     /translation="MYSSSRKRCPKTKWALKLLTAAFLAASPAAKSAVNNAYDALIIE
                     ARKGNTQPALSWFALKSALSNNQIADWLQIALWAGQDKQVITVYNRYRHQQLPARGYA
                     AVAVAYRNLQQWQNSLTLWQKALSLEPQNKDYQRGQILTLADAGHYDTALVKLKQLNS
                     GAPDKANLLAEAYIYKLAGRHQDELRAMTESLPENASTQQYPTEYVQALRNNQLAAAI
                     DDANLTPDIRADIHAELVRLSFMPTRSESERYAIADRALAQYAALEILWHDNPDRTAQ
                     YQRIQVDHLGALLTRDRYKDVISHYQRLKKTGQIIPPWGQYWVASAYLKDHQPKKAQS
                     IMTELFYHKETIAPDLSDEELADLFYSHLESENYPGA"
BASE COUNT          379 a          312 c          291 g          348 t
ORIGIN      
        1 gccgtattcc tgaagatcct catcattgga atggattttc gggcgagaaa aggattttat
       61 atggacactc tgctcatcat ttcttcttct catcatcaac aattcacgtc tctcttccgc
      121 gtttaataac ggattatgag gtgcaaaaat atctttcttt tcagttacct gtaattagat
      181 acagagagag attttggcaa tacatggagt aatacaggat gtattcaagt agcagaaaaa
      241 ggtgcccgaa aaccaaatgg gctttgaaac ttcttactgc cgcattttta gcagcgagtc
      301 ccgcggcgaa gagtgctgtt aataacgcct atgatgcatt gattattgaa gctcgcaagg
      361 gtaatactca gccagctttg tcatggtttg cactaaaatc agcactcagc aataaccaaa
      421 ttgctgactg gttacagatt gccttatggg ccgggcaaga taaacaggtt attaccgttt
      481 acaaccgcta ccgtcatcag caattaccag cgcgtggtta tgcagctgtc gccgtcgctt
      541 atcgtaacct gcaacaatgg caaaactcgc ttacactgtg gcaaaaggcg ctctctctgg
      601 agccgcaaaa taaggattat caacggggac aaattttaac cctggcagat gctggtcact
      661 atgatactgc gctggttaaa cttaagcagc ttaactctgg agcaccggac aaagccaatt
      721 tactcgcaga agcctatatc tataaactgg cggggcgtca tcaggatgaa ttacgggcga
      781 tgacagagtc attacctgaa aatgcatcta cgcaacaata tcccacagaa tacgtgcagg
      841 cattacgtaa taatcaactt gctgccgcga ttgacgatgc caatttaacg ccagatattc
      901 gcgctgatat tcatgccgaa ctggtcagac tgtcgtttat gcctacgcgc agtgaaagtg
      961 aacgttatgc cattgccgat cgcgccctcg cccaatacgc tgcattagaa attctgtggc
     1021 acgataaccc agaccgtact gcccagtacc agcgtattca ggttgatcat cttggcgcgt
     1081 tattaactcg cgatcgttat aaagacgtta tttctcacta tcagcgatta aaaaagacgg
     1141 ggcaaattat tccgccctgg gggcaatatt gggttgcatc ggcttatctc aaagatcatc
     1201 agccgaaaaa agcacagtca ataatgaccg agctctttta tcacaaggag accattgccc
     1261 cggatttatc cgatgaagaa cttgcggatc tcttttacag ccacctggag agtgaaaatt
     1321 atccgggcgc
//
LOCUS       DAPHDW010000317         1305 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.324, whole genome shotgun sequence.
ACCESSION   DAPHDW010000317 DAPHDW010000000
VERSION     DAPHDW010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1305
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.324"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..885
                     /locus_tag="RH057_004579"
     CDS             <1..885
                     /locus_tag="RH057_004579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV3130795.1"
                     /translation="FINKMWDGSVLSVDRKNEPEQMIENARMTLSLMVQPGLFDRYME
                     RKGSVARDSGFLARCLISKPATTQGKRFINGAVIPGGSLTAFHERLMELARGSIEKSS
                     EDERYCLHFSPEAQKIFIEHYNVLEQDLSPSGPLSPFRGHVSKKTENIARIAALFQYF
                     SYGEGKISADIMTSAVVISSWYTDEYKKLFALPDESELQQKDAEELFDWLIEECRGEC
                     PPRVRKNYILQCGPGRFRNRKKLNALLNILESQFRLSVVPEGKTMYVLLPQIASLKLS
                     DVSGIFTSGYHYNKLRAK"
     gene            1101..1301
                     /locus_tag="RH057_004580"
     CDS             1101..1301
                     /locus_tag="RH057_004580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001427457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130796.1"
                     /translation="MQRVTERTESETAGRQAVQSALAPCPDTSFPETTGIRCAYRQPL
                     CGMPGARTDPSDPSVLRSASDG"
BASE COUNT          345 a          284 c          331 g          345 t
ORIGIN      
        1 tttattaaca aaatgtggga tggctctgta ttgtctgtgg acagaaagaa tgaacccgaa
       61 cagatgattg aaaatgccag aatgacgtta tccctcatgg tgcaacccgg tctttttgac
      121 cgatatatgg agcgaaaagg ttctgttgcc agggacagtg gttttcttgc ccgttgtctt
      181 atcagtaaac ctgcaaccac gcaggggaaa cgctttatta acggagctgt gattccggga
      241 ggatcattaa cggcatttca tgaacgatta atggaactgg ccagaggcag tattgaaaaa
      301 tccagtgagg atgaacggta ttgtctgcat ttttcgcctg aagcgcaaaa aatatttatt
      361 gagcactata atgttctgga gcaggattta tccccttccg gacccttaag cccgtttcgg
      421 ggacatgttt caaaaaaaac agaaaacatt gcaagaattg ctgcgttatt tcagtatttc
      481 agctatggtg aagggaaaat ctctgccgat atcatgacgt ctgctgtcgt aataagttcg
      541 tggtacacag atgaatataa aaaattattt gcgttacctg atgaatctga actgcagcag
      601 aaggacgccg aggagctgtt cgactggctc attgaggagt gccgcggaga atgcccgccc
      661 cgtgtcagga aaaattatat tctccagtgt ggcccgggcc ggttcagaaa caggaaaaaa
      721 ctgaacgcat tgctgaacat tcttgaatct cagttcaggc tttctgtcgt gccggaagga
      781 aaaaccatgt atgttctgct tccccagata gccagtttaa agttgtctga tgtctctggt
      841 atttttacct cgggttatca ctacaataag ctgagagcga agtaaccggt gagcagtttt
      901 tctccattat gcttcagact tagatgattg attattatgg ggatttcatc accgatattc
      961 agatgtatga atatcggtga tgaactaaag ctcaggagct gcgtggtggt gatgaatcaa
     1021 caggcatata actgataccc gccggtcaga agttccagcc tgcgactttc ctgtctgcgt
     1081 gcccgcagtc cggcgttcag gtgcagcgtg tgacagaacg cacggagagc gaaacggcag
     1141 gcagacaggc cgttcagtca gccctggcac cctgcccgga taccagcttc cctgaaacaa
     1201 ctggcatcag gtgcgcttat cgtcaaccgt tgtgcggtat gccaggagca cgtactgatc
     1261 catcagatcc gtcagtgctg cgctctgctt ctgacggttg atgat
//
LOCUS       DAPHDW010000318         1303 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.325, whole genome shotgun sequence.
ACCESSION   DAPHDW010000318 DAPHDW010000000
VERSION     DAPHDW010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1303)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.325"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            728..919
                     /locus_tag="RH057_004581"
                     /pseudo
     CDS             728..919
                     /locus_tag="RH057_004581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1014..1202)
                     /locus_tag="RH057_004582"
     CDS             complement(1014..1202)
                     /locus_tag="RH057_004582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000654305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FaeA/PapI family transcriptional regulator"
                     /protein_id="HDV3130797.1"
                     /translation="MKDNILTFLTELKTPCRTTEIAIHFGLSAYQARYYLMTLEKEGK
                     IKRSPVRRGASTLWEMNS"
BASE COUNT          322 a          285 c          293 g          403 t
ORIGIN      
        1 tttcattctg cttttaattt tattcatttt tattgaatac ctgacgtgta tatttatgag
       61 tacaaactgc taaacaaaac acgatccact ttcattgtaa tatacatccg gagctatgtt
      121 ggactttgta tcgagtttga aaaaattcca agttatggaa gtctaccatt ttgggcagaa
      181 aaaccattta ctatgactaa atataatgat catcgtcaca aaaaagaatt tgagtgtgta
      241 gtcttgtctt ttaggagcag tccagtcatt ggcgaccaca tgttgttgaa cggcatcggt
      301 acttactggt gctaccgaaa cgctgtctgg aataactcct ggctcttttg cctgagggcc
      361 ctgaacccgc ctttgagctt gttcatcatg caattattgg ttcaccctat attgtgaacg
      421 ccgattgggt ttgaacaggc ctgctgatca tcggactaat tgcactatcc ccgattctga
      481 ctgcttcggg gattaacagc ggcttgttgc ctttaggaac aattttttag atcctgttaa
      541 tcaaacgtac tggtgagcgg ctgggccaat ccgtgatcga atagagcgga atacgcagtg
      601 tagtgacttt taacgtcagg tgatgcataa gggcctttcg ctgagagacc ctgtttacac
      661 aattaaataa gtcaagcccg accagcaatt ttgacgttga cggcaggatt attgaggggc
      721 aatcaatgtg ttattcggca gcgattacgg tagtgaacgg cgctctactg tacggactga
      781 gcgggacctg ctgtctgaat ggtatctgcg ccatatcctg agcgtactgc cggaatggcc
      841 ttccagccgt gttgatgaac tcctgctgca tattagcatg gaggtgttcg gtgagtgccg
      901 gacgacgtat tctctgtaat aattcttaca cctgcgaaat ggcatggttc atcgtctgac
      961 aacccgggaa aaccgttgac catttttata tttcaggcac agatattttc ccgtcacgaa
     1021 ttcatttccc ataaggtact ggcacctctc cttactggcg agcgttttat ctttccctcc
     1081 ttttcgagag tcatcaggta atatctggcc tgataggcac tcagcccaaa atgtatggca
     1141 atttctgttg tccggcacgg agttttcagc tccgtgagga acgtcagtat attgtctttc
     1201 atattctgtt aattcccgtg tggacctgaa tatcgtgcat ggtatatcaa accatacgaa
     1261 ctcttgtcgt tgttaatttc agccatgtaa agccatcagg gct
//
LOCUS       DAPHDW010000319         1300 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.326, whole genome shotgun sequence.
ACCESSION   DAPHDW010000319 DAPHDW010000000
VERSION     DAPHDW010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1300)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1300)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1300
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.326"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..447)
                     /locus_tag="RH057_004583"
     CDS             complement(<1..447)
                     /locus_tag="RH057_004583"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130798.1"
                     /translation="MIAFDTQRLRDFMAGLVVFIICVILAVLTFRKVSNYSRNKGRGK
                     FRTLITSLGMSFIIFVVSVGIGSGVVSQDKSTDVKASTSTSTEKAKTNVYKCDKFDAI
                     IQTRQGTKSDSGYYSDKGILIYYTISDNELISRMPIPGDKDSVYTAK"
     gene            981..>1300
                     /locus_tag="RH057_004584"
     CDS             981..>1300
                     /locus_tag="RH057_004584"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130799.1"
                     /translation="MDENNKEVIEIRPEDKDFFAEFIDCEGPIIQDSIDHKKITLALD
                     KAHDIRKFEIDLYWKRATYFFAFFTVITAAFGYLFTHDNYTFLAPALAIIGSVFALCF
                     CYVN"
BASE COUNT          390 a          254 c          250 g          406 t
ORIGIN      
        1 tttagcagta tacacactat ctttatcgcc gggaattggc attcgactga ttaattcatt
       61 atcggaaata gtataataaa ttaaaattcc tttatctgaa tagtaacctg aatcgctttt
      121 tgttccctgt cgagtttgta ttattgcatc gaacttatcg catttataaa cattggtttt
      181 ggccttttca gtactggtag acgttgaagc ctttacatca gtacttttat cttgtgaaac
      241 aaccccggaa ccgataccta ccgaaacgac aaagataata aaactcatgc ctaacgacgt
      301 aatcaaagta cggaactttc cccgaccttt atttctggaa tagtttgaaa ctttcctgaa
      361 agtaagtact gcaaggatca cacaaatgat gaaaacgaca agcccggcca taaaatccct
      421 caaacgttgt gtatcaaagg caatcatagt atcacttcgc aatacattga tcgattaaag
      481 cgatcaattt ttgattttcg atcggttaaa tctatcaata tctattaatt gaagacaaat
      541 caataggtaa aaacgatcag acgttggtca gtacttcgga aacaccagaa gtatggtttg
      601 ggtctgccgt ttaaacgccc tcagaaaacc gctaaggtct ctttatcgca tatggcttgg
      661 gatttcagcc tttggctaaa atcgctttca ggggggcttc ttgtgcgttt tgtcgggaac
      721 accatcatgg agggggataa atcggatcga agtcgcttcg ctctggtcag tactggaagg
      781 gttagcttat ctggcgaggg aagatgaatt gccaggcttc atgaagatgg ttggttttgt
      841 ggacaagaag aactgccgtt ttgagtattc cccctcacac ccaacaaacc tacccattag
      901 caagggggag tactttctcg tagtatacag aacgaataaa ttctttgtat acttccgttt
      961 tatcctaaag cgatttgatt atggacgaga ataataaaga agttattgaa ataagaccag
     1021 aagataagga ctttttcgca gaatttattg attgtgaagg tccaattatc caagattcaa
     1081 ttgatcataa aaaaatcacc ctcgcacttg ataaagccca cgacataaga aagtttgaaa
     1141 tagatctcta ttggaagcgt gctacatact tcttcgcatt cttcactgta ataacagctg
     1201 catttggtta tttgttcacc catgacaact atactttctt agcccctgca cttgcgatca
     1261 ttggttctgt ttttgcttta tgtttttgtt atgtgaatat
//
LOCUS       DAPHDW010000320         1296 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.327, whole genome shotgun sequence.
ACCESSION   DAPHDW010000320 DAPHDW010000000
VERSION     DAPHDW010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1296)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1296)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1296
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.327"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            165..767
                     /locus_tag="RH057_004585"
     CDS             165..767
                     /locus_tag="RH057_004585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HDV3130800.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADIPDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDATFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            complement(806..1231)
                     /gene="nudI"
                     /locus_tag="RH057_004586"
     CDS             complement(806..1231)
                     /gene="nudI"
                     /locus_tag="RH057_004586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HDV3130801.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
BASE COUNT          355 a          284 c          272 g          385 t
ORIGIN      
        1 tttttatatt ttttcttatt taggctttgc atttggcaaa attttgaggc attttgccga
       61 catcgtagga tttttaatat tacaccaact gctaattatc gccagaaatg tcgctcaatt
      121 ttacggtaat tgtctggttg cgcttgtcta taggtggagt ttacgtgtta gctttttgcc
      181 gctcatcatt gaagtcaaaa aaatatttca tcattttact ggcgctcgct gcaattgccg
      241 gactgggtac tcatgccgcc tggagtagca atggtttgcc acgtatcgac aataaaacat
      301 tggccagact ggcacagcag cacccggttg tcgttttgtt tcgccatgct gaacgttgcg
      361 atcgttcaac caatcaatgc ttgtcagata aaacaggtat tacggttaaa ggtacccagg
      421 atgcccgtga actgggcaac gcttttagcg ctgacatccc tgatttcgat ctttattcca
      481 gtaataccgt ccggaccatt cagtcggcga cctggttttc cgcgggtaaa aagctgacgg
      541 tagataaacg acttcttcag tgcggtaatg agatttatag tgcaattaag gacttacaaa
      601 gcaaagcgcc tgataaaaat atcgttattt tcacccataa tcattgcctg acatatattg
      661 ctaaaaataa gcgtgatgcg acatttaaac ctgattatct ggatggttta gtcatgcatg
      721 tggaaaaagg caaagtttat ctggatgggg aattcgttaa ccactaaatt ttcaatctga
      781 cagccagtga tggctgtcat cattgttaaa gaagaccttt caaacgtaac gtttttcggg
      841 tggcaacatt taaatcataa tgcaccagat cttcaggttt tacccacgcg tagtcctgaa
      901 actcttcgtt tattttcact tctcggttgg cagaaacgca gtcaaaaatc aggtaaatca
      961 tataaatctc ttccttgcga ccatctgcat acgtcttggt gcgaatatca tcgctgaagg
     1021 tccacggcgt gatttctgtc aaaagcagct gttctcccag ttcttcgcga atttcgcggc
     1081 gtagtgcctc ttcaattcgt tcgccagact ccacgccgcc acccgaaagc gcccattgac
     1141 cggggaaaac gccgcgatcg tcggccattt tacacagcaa ataagcacca tcattttgaa
     1201 tcaaagggca tacaatagtc cgttgtcgca taggggtatt ccttataaat aaaaagaaac
     1261 catgagaata cggtaactca gaaatatgtg aaagtg
//
LOCUS       DAPHDW010000321         1285 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.328, whole genome shotgun sequence.
ACCESSION   DAPHDW010000321 DAPHDW010000000
VERSION     DAPHDW010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1285
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.328"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1285
                     /locus_tag="RH057_004587"
     rRNA            <1..>1285
                     /locus_tag="RH057_004587"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          320 a          298 c          408 g          259 t
ORIGIN      
        1 gtaggtgggg taacggctca cctaggcgac gatccctagc tggtctgaga ggatgaccag
       61 ccacactgga actgagacac ggtccagact cctacgggag gcagcagtgg ggaatattgc
      121 acaatgggcg caagcctgat gcagccatgc cgcgtgtatg aagaaggcct tcgggttgta
      181 aagtactttc agcggggagg aagggagtaa agttaatacc tttgctcatt gacgttaccc
      241 gcagaagaag caccggctaa ctccgtgcca gcagccgcgg taatacggag ggtgcaagcg
      301 ttaatcggaa ttactgggcg taaagcgcac gcaggcggtt tgttaagtca gatgtgaaat
      361 ccccgggctc aacctgggaa ctgcatctga tactggcaag cttgagtctc gtagaggggg
      421 gtagaattcc aggtgtagcg gtgaaatgcg tagagatctg gaggaatacc ggtggcgaag
      481 gcggccccct ggacgaagac tgacgctcag gtgcgaaagc gtggggagca aacaggatta
      541 gataccctgg tagtccacgc cgtaaacgat gtcgacttgg aggttgtgcc cttgaggcgt
      601 ggcttccgga gctaacgcgt taagtcgacc gcctggggag tacggccgca aggttaaaac
      661 tcaaatgaat tgacgggggc ccgcacaagc ggtggagcat gtggtttaat tcgatgcaac
      721 gcgaagaacc ttacctggtc ttgacatcca cggaagtttt cagagatgag aatgtgcctt
      781 cgggaaccgt gagacaggtg ctgcatggct gtcgtcagct cgtgttgtga aatgttgggt
      841 taagtcccgc aacgagcgca acccttatcc tttgttgcca gcggtccggc cgggaactca
      901 aaggagactg ccagtgataa actggaggaa ggtggggatg acgtcaagtc atcatggccc
      961 ttacgaccag ggctacacac gtgctacaat ggcgcataca aagagaagcg acctcgcgag
     1021 agcaagcgga cctcataaag tgcgtcgtag tccggattgg agtctgcaac tcgactccat
     1081 gaagtcggaa tcgctagtaa tcgtggatca gaatgccacg gtgaatacgt tcccgggcct
     1141 tgtacacacc gcccgtcaca ccatgggagt gggttgcaaa agaagtaggt agcttaacct
     1201 tcgggagggc gcttaccact ttgtgattca tgactggggt gaagtcgtaa caaggtaacc
     1261 gtaggggaac ctgcggttgg atcac
//
LOCUS       DAPHDW010000322         1230 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.329, whole genome shotgun sequence.
ACCESSION   DAPHDW010000322 DAPHDW010000000
VERSION     DAPHDW010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.329"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..776
                     /locus_tag="RH057_004588"
     CDS             <1..776
                     /locus_tag="RH057_004588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HDV3130802.1"
                     /translation="EHWHSFEGYAENQYFVDPCKLLLTTPGIDARNGEYEAFGVPATI
                     LANFLRENGVVPEKCDLNSILFLLTPAEDMAKLQQLVALLVRFEKLLESDAPLAEVLP
                     SIYKQHEERYTGYTLRQLCQEMHDLYARHNVKQLQKEMFRKEHFPRVSMNPQEANYAY
                     LRGEVELVRLPDAEGRIAAEGALPYPPGVLCVVPGEIWGGAVLRYFSALEEGINLLPG
                     FAPELQGVYIEEHDGRKQVWCYVIKPRDAQSTLLKGEKL"
     gene            773..>1230
                     /locus_tag="RH057_004589"
     CDS             773..>1230
                     /locus_tag="RH057_004589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HDV3130803.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMYSRKSGGMGGYAEYAFGKSGNFMANYTYGVSLLIANVA
                     IAISAVGYGTELFGASLSPVQIGLATIGVLWICTVANFGGARITGQISSIT"
BASE COUNT          282 a          300 c          340 g          308 t
ORIGIN      
        1 gggaacactg gcattctttt gaaggctatg cagagaatca atactttgtc gatccttgca
       61 aactgttgct gacaacccca ggtattgatg cacgtaacgg cgaatatgaa gcgttcggcg
      121 tacccgcgac gattcttgct aacttcctgc gcgaaaatgg cgtagtgccg gaaaaatgcg
      181 atcttaactc catcctcttc ctgctgactc cggcagaaga tatggccaaa ctgcagcaac
      241 ttgttgccct gctggtacgc ttcgaaaaac tgcttgagtc cgacgcgcca ttagcagaag
      301 tgctgccttc catctacaaa cagcatgaag agcgctacac cggttatacc ctgcgtcagt
      361 tgtgtcagga aatgcacgat ttgtatgccc gccacaacgt gaaacaactg caaaaagaga
      421 tgttccgtaa ggaacacttc ccacgcgtca gcatgaatcc acaggaagcc aactacgcct
      481 atttacgcgg tgaagtggag ctggttcgtc tgccggatgc cgaaggccgt atcgctgccg
      541 agggtgcgct tccttatcct ccgggtgtgc tgtgtgttgt tccgggtgag atctggggtg
      601 gtgctgttct gcgttacttc agcgctctgg aagaagggat caacctgctg ccaggttttg
      661 caccggagct gcagggtgtc tatatcgaag aacatgatgg tcgtaagcaa gtctggtgct
      721 atgtcatcaa gccccgtgat gcgcaaagca ccctgttaaa aggggaaaaa ttatgagtca
      781 ggctaaatcg aacaagatgg gcgttgttca gttaaccata ctgacgatgg tcaacatgat
      841 gggctctggc atcatcatgc tgccgactaa actggcagag gtcgggacga tctcgattat
      901 ctcctggctg gttacagctg taggctcaat ggcgctggcc tgggccttcg caaaatgcgg
      961 tatgtatagc cgtaaatcag gtggtatggg cggttatgcc gaatatgcgt tcggtaaatc
     1021 cggtaacttt atggcgaact acacctacgg tgtctcgctg ctgattgcca atgtcgccat
     1081 tgcgatttcc gccgttggtt acggcaccga actgttcggc gcgagtttgt cgccagtgca
     1141 gattggtctt gcgaccatcg gagtgctgtg gatttgtact gtggctaact ttggtggtgc
     1201 gcgcattacc gggcaaatca gtagcattac
//
LOCUS       DAPHDW010000323         1216 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.330, whole genome shotgun sequence.
ACCESSION   DAPHDW010000323 DAPHDW010000000
VERSION     DAPHDW010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.330"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            52..>1216
                     /locus_tag="RH057_004590"
     CDS             join(52..310,310..>1216)
                     /locus_tag="RH057_004590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611881.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HDV3130804.1"
                     /translation="MNKKTKRTFTPEFRLECAQLIVDKGYSYRQASEAMNVGSTTLES
                     WVRQLRRERQGITPSATPITPDQQRIRELEKQVRRLEEQNTIFKKGYRALNVRLAERF
                     TIAARLSDSHTVVSLCSALEIHRSSYRYWRKRRDTVNPARVRLCSEIRRAWNQSRGSA
                     GARTLAEMLTQNGVPMSRYRAGRLMKYLNLSSCQPGKHQYKNARQEHTSLPNLLERQF
                     AVPEPDRVWCGDITYLWAGNRWCYLAVVMDLFARRVIGWSLSAHADTALISSALRMAY
                     ETRGQPRDVVFHSDQGSQYTGLKYQQLLWRCRINQSVSRRGNCWDNSPMERFFRSLKT
                     EWVPTNGYAGKDEARQQINDYILNYYNSVRPHHYNGGLTPEESENRYHFYCKTVA"
BASE COUNT          328 a          314 c          329 g          245 t
ORIGIN      
        1 gccacctgat gaaaggtgat attctcacca caacacaaaa caggtgactt aatgaacaag
       61 aaaaccaaac gaaccttcac ccctgagttc aggctggaat gtgcacagct gattgttgat
      121 aagggctact catatcgaca ggccagtgaa gcgatgaatg tcggttcaac cacgcttgag
      181 agctgggtac gccagctcag gcgagagcgc cagggtatta cgccctctgc cacacccatt
      241 actccagacc agcaacgtat ccgcgagctg gaaaagcaag ttcgccgtct ggaggaacaa
      301 aatacgatat taaaaaaggc taccgcgctc ttaatgtccg actcgctgaa cggttcacga
      361 tagccgccag actaagtgac agccacacgg ttgtcagcct gtgttctgct ctggaaatac
      421 accgcagcag ttaccggtac tggcgaaaac gacgcgatac ggttaatccg gcacgagtca
      481 ggttgtgcag cgaaatacgc cgggcgtgga accaaagtcg gggctctgcg ggcgcgcgca
      541 ctctggctga aatgctgact caaaacggcg tcccgatgag ccgttaccgt gccggacgtc
      601 tgatgaaata tctgaacctg agcagttgtc agcccggaaa acatcagtat aaaaatgccc
      661 gtcaggagca taccagcctg ccgaatctgc ttgagcgtca gttcgcagta ccggagccag
      721 accgggtatg gtgtggagat attacgtatc tctgggcagg aaatcgctgg tgctatctgg
      781 cggttgttat ggatcttttt gcccgcaggg ttatcggctg gagtctgtca gcgcatgccg
      841 ataccgcact gataagcagt gccctgcgga tggcctatga gacgcgtggc caaccccggg
      901 atgtcgtgtt ccatagcgac cagggaagcc agtatacagg ccttaaatat caacaacttc
      961 tctggcgttg cagaataaat caaagcgtca gtcggcgggg aaactgctgg gataacagcc
     1021 ccatggaacg cttcttccgc agtctgaaaa cagaatgggt gccaacgaat ggttacgcag
     1081 gcaaggacga ggcccggcag caaattaatg attacatatt gaactactac aacagcgtca
     1141 gacctcacca ttataacggt gggctgacgc cggaagagtc agagaacaga taccattttt
     1201 actgtaaaac cgtggc
//
LOCUS       DAPHDW010000324         1198 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.331, whole genome shotgun sequence.
ACCESSION   DAPHDW010000324 DAPHDW010000000
VERSION     DAPHDW010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1198
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.331"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            121..555
                     /locus_tag="RH057_004591"
     CDS             121..555
                     /locus_tag="RH057_004591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001362728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence hypothetical
                     protein"
                     /protein_id="HDV3130805.1"
                     /translation="MDKPTDWRSGTRRIFSNEFKLHMVELASKPNANVAQLAREHGVD
                     NNLIFKWLRLWQREGRISRRMPPTIVGPTVSQSFPASPTLVPVELIDTPRCATDAPAP
                     EALSVACAASCHVEFHYGKMMLENPSPELLTVLIRELTGRGR"
     gene            552..902
                     /gene="tnpB"
                     /locus_tag="RH057_004592"
     CDS             552..902
                     /gene="tnpB"
                     /locus_tag="RH057_004592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017901265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HDV3130806.1"
                     /translation="MISLPSGTRIWLVAGVTDMRKSFNGLGEQIQHVLDDNPFSGHLF
                     IFRGRRGDTIKILWADADGLCLFTKRLEEGQFIWPAVRDGKISITRSQLAMLLDKLDW
                     RQPKTSRLNALTML"
     gene            933..>1198
                     /locus_tag="RH057_004593"
     CDS             933..>1198
                     /locus_tag="RH057_004593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HDV3130807.1"
                     /translation="MSQKYLIRIAELERLLSEQAEALRQKDQQLSLVEETEAFLRSAL
                     ARAEEKIEEDEREIEHLRAQIEKLRRMLFGTRSEKLRREVEQAEA"
BASE COUNT          309 a          305 c          296 g          288 t
ORIGIN      
        1 ccagacaacc aatatcactt aaataagtga tagtcgtaag cgtaaagcca atgccgtctg
       61 taacacctgc tccttgcaga ctaaattaga gctccttcta aattagacgg agttctattg
      121 atggataaac ctacagactg gcgctccgga acccgccgga tattttctaa tgaatttaaa
      181 cttcatatgg ttgaactggc ttcgaaacca aatgccaatg ttgcacaact ggcccgggaa
      241 catggcgttg ataacaacct gatttttaaa tggctacgcc tctggcaaag agaaggacgt
      301 atttctcgta gaatgcctcc aactattgta ggccctacag tatcacaatc ttttccggcc
      361 tctccgactc tggttccagt ggaacttatc gacaccccgc gctgtgctac agatgctcct
      421 gctccggagg cattatcagt tgcttgtgca gcttcctgcc atgtggaatt ccattacggt
      481 aaaatgatgc tggaaaatcc ttcaccagag ctgctcacgg tgttgatccg tgagctgacc
      541 gggaggggac gatgatttca ctcccatcag gtacccgtat ctggctcgtt gccggcgtta
      601 ctgatatgcg taaatccttc aacggtctgg gggagcagat acagcatgtg ctggatgata
      661 accccttctc cggtcacctg tttatcttcc gtggccgacg gggagacacg attaaaatcc
      721 tgtgggctga tgctgatggt ctgtgcctgt tcaccaaacg ccttgaggaa ggtcagttta
      781 tctggcctgc ggtgcgtgac ggtaagatat ccattacccg ctcgcaactg gcaatgctcc
      841 tcgataagct ggactggcgt cagccaaaaa catcccgcct taacgcactg acaatgttgt
      901 aaaaaactcc tgaccgcatt ataaaaacgg ccatgagtca gaaatacctc attcgcatcg
      961 ctgagctgga aaggctgctc tctgagcagg ctgaagccct ccgtcagaaa gaccagcaac
     1021 tgagtctggt tgaagagacg gaggccttcc tgcgctctgc actggcacgt gccgaagaaa
     1081 agatcgaaga agatgaacgg gaaatagagc atctgcgggc tcagatagaa aaactgcgcc
     1141 ggatgctgtt cggaacccgt tctgaaaaac tgcgtcgtga ggttgaacag gctgaagc
//
LOCUS       DAPHDW010000325         1177 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.333, whole genome shotgun sequence.
ACCESSION   DAPHDW010000325 DAPHDW010000000
VERSION     DAPHDW010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.333"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            108..866
                     /locus_tag="RH057_004594"
     CDS             108..866
                     /locus_tag="RH057_004594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HDV3130808.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            complement(919..1026)
                     /locus_tag="RH057_004595"
                     /pseudo
     CDS             complement(919..1026)
                     /locus_tag="RH057_004595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          289 a          273 c          283 g          332 t
ORIGIN      
        1 gcgccttttt ataattcgtt acctttccag ggacgctaat ctttgatcct taagaaagtt
       61 tccgggaccg cgatggtctc tttttttgtt gtacggactc ttgataaatg aagcttttga
      121 agacagttcc tgccatcgtt atgttggcgg ggggcatgtt tgcgtcactg aatgcagctg
      181 ccgatgattc cgtttttact gtcatggacg atcctgccag cgctaaaaaa cctttcgaag
      241 gtaatctgaa cgcaggatat cttgcacaat caggcaatac gaaaagctcc tcacttacgg
      301 ctgacaccac catgacctgg tatggacaaa ccactgcctg gtcgctgtgg gggaacgcca
      361 gtaatacttc ttctaatgat gagcgttctt cagaaaaata tgcggcgggc gggcgtagcc
      421 gttttaattt aactgattat gactatttat ttggtcaggc aagctggctg acagaccgtt
      481 ataacggcta tcgagagcgc gatgtgttga ccgcgggtta tggtcgtcag tttttgaatg
      541 gcccggtaca cagtttccgc tttgaattcg gtccaggtgt gcgttacgac aaatataccg
      601 acaatgccag tgaaactcag ccgctgggct atgcctccgg tgcttatgca tggcagttga
      661 ctgacaacgc taaatttacg cagggtgttt ccgtctttgg cgcggaagac acaacgctga
      721 actccgaaag cgcattaaat gttgctatta atgaacattt tggactgaaa gtggcttaca
      781 acgtcacatg gaactctgaa cctccagagt ctgcaccgga acataccgat cgtcggacaa
      841 ccttatcgct gggctacagc atgtaaaatt atctgccgga ccgtgttatg cggtctggct
      901 gtcttcgttt gtcactgctc agccccaaat cattgctgcc caacgtagca acaacatagc
      961 gccacagatt gccagtagca ccaccaccat ctttcagtgt tttttatcaa agcgtttagt
     1021 cggcatacca gtgtcctgca tatctcttgt gcgtgataac ggagtgtaac aaaagggaca
     1081 ggcatcaagg gattttttgt tattacagcc gtcgggtggc gttgtacacc tgatcactgt
     1141 ttctgcgttt tatgaaaaaa ataaaattat gtatcga
//
LOCUS       DAPHDW010000326         1171 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.334, whole genome shotgun sequence.
ACCESSION   DAPHDW010000326 DAPHDW010000000
VERSION     DAPHDW010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1171)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1171)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1171
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.334"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..482)
                     /gene="sfmF"
                     /locus_tag="RH057_004596"
     CDS             complement(<1..482)
                     /gene="sfmF"
                     /locus_tag="RH057_004596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria assembly protein"
                     /protein_id="HDV3130809.1"
                     /translation="MRRALFSCFCGLLWISSGWAADPLGTININLHGNVVDFSCTVNT
                     ADIDKTVDLGRWPTTQLLNAGDTTALVPFSLRLEGCPPGSVAILFTGTPASDTNLLAL
                     DDPAMAQTIAIELRNSDRSRLALGAASPTEEVDANGNVTLNFFANYRALASGVRPGVA
                     "
     gene            complement(493..>1171)
                     /gene="fimH"
                     /locus_tag="RH057_004597"
     CDS             complement(493..>1171)
                     /gene="fimH"
                     /locus_tag="RH057_004597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 1 fimbrin D-mannose specific adhesin FimH"
                     /protein_id="HDV3130810.1"
                     /translation="VDSVMDIQFPPQNYIRMGTDPNVSQNLPFGVMDSRLIFRLKVIR
                     PFINMVEIPRQVMFTVYVTSTPYDPLVTPVYTISFGGRVEVPQNCELNAGQIVEFDFG
                     DIGASLFSAAGPGNRPAGVMPQTKSIAVKCTNVAAQAYLTMRLEASAVSGQAMVSDNQ
                     DLGFIVADQNDTPITPNDLNSVIPFRLDAAAAANVTLRARPISITGQKPTEGPFSALG
                     YLRVDYQ"
BASE COUNT          319 a          311 c          268 g          273 t
ORIGIN      
        1 ttcgccacac ctggccgaac accgctggct aacgctcgat aattggcaaa aaagtttagt
       61 gtgacattgc catttgcatc tacttcctca gtcgggctcg ccgctcccag tgcgagccgg
      121 gagcgatcgc tattacgtaa ttcgatggcg atggtttgcg ccattgcggg atcatccaga
      181 gccagcaggt tggtatcgga tgccggcgtt cccgtaaata aaatcgcaac tgaacccgga
      241 ggacatccct ccagccgcag gctaaaaggg acgagtgccg tggtatcgcc agcgttcagt
      301 aattgtgtcg taggccatct gcctaaatct accgtcttat caatatccgc agtgtttacg
      361 gtacaggaga aatcaacaac gttaccgtgc aaattgatat taatcgttcc caaagggtca
      421 gctgcccatc cactggaaat ccacagtaac ccgcagaaac agctaaagag tgctcttctc
      481 attctccgta cctcattgat aatcgacgcg taaatacccc agcgcgctaa acggcccttc
      541 ggtcggtttt tgaccggtaa tactgatagg cctggcgcga agtgtgacat tggctgccgc
      601 agctgcatcc agacggaaag gaataacgct attgagatcg ttaggcgtga tcggcgtatc
      661 gttctgatcg gcgacaataa aacctaaatc ctgattgtcc gacaccatcg cctgaccaga
      721 aacggcactg gcttccagac gcattgttaa ataagcctgc gcagcaacat tcgtacattt
      781 gaccgcaatg ctcttggttt gcggcatgac accagcaggt cgattacccg gccctgccgc
      841 actaaataac gatgcgccga tatcaccaaa atcaaattca acaatctgcc cggcatttaa
      901 ttcgcagttt tgcggtactt caacccggcc accaaaacta atggtataaa caggtgtaac
      961 caacggatcg taaggcgttg atgtcacata cacggtaaac atcacctgtc tggggatctc
     1021 caccatgtta ataaagggac gaataacctt taaacgaaat attaaacgag aatccatcac
     1081 cccgaatgga aggttttgtg aaacgttagg atctgttccc atccgaatat aattttgcgg
     1141 ggggaactga atatccatca cactatcaac c
//
LOCUS       DAPHDW010000327         1167 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.335, whole genome shotgun sequence.
ACCESSION   DAPHDW010000327 DAPHDW010000000
VERSION     DAPHDW010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1167)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1167)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1167
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.335"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..267)
                     /locus_tag="RH057_004598"
     CDS             complement(<1..267)
                     /locus_tag="RH057_004598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HDV3130811.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQEFSEYIAPLQD
                     AVDLEIATEEERSLLEAWNKYRVLLNRVDTSVALGMKLFVTHVTV"
     gene            324..992
                     /locus_tag="RH057_004599"
     CDS             324..992
                     /locus_tag="RH057_004599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HDV3130812.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     INHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
BASE COUNT          347 a          265 c          213 g          342 t
ORIGIN      
        1 tactgtaaca tgagttacaa acagcttcat acccagagct actgatgtat caactcgatt
       61 caacaatacc cgatatttat tccatgcctc cagcaacgat ctttcttcct ccgttgcgat
      121 ttccagatct acagcatcct gcagtggcgc aatatactca ctgaattcct ggatgtagaa
      181 ctgtgtagtg acggtcttcc agccattcgg ctcctgctgt atcgaagcat accaggctat
      241 ttcaatatcg ctatgctgcg gcagcattta accccttgta attcatcgcc ataattgatt
      301 taattcacaa ataaaactat aacatggtga aattaatgaa aaaaaacaca gatgatgggg
      361 ctaaaattta cacaccactt accctaaagc tttatgactg gtgggttttg ggagtatcaa
      421 atcggcttgc atggggatgt cctacaaagg aacaccttct tccacacttt ctggaacatt
      481 taggtaacaa ccatctggat attggtgttg gaactgggtt ttaccttact cacgtacctg
      541 agagtagtct gatatcttta atggatttga acgaagctag cctgaacgcg gcatctacaa
      601 gggctgggga atcaaaaatt aaacataaaa ttaaccatga tgtttttgaa ccttatcccg
      661 cggcgttaca tggtcaattt gattccattt ccatgtttta ccttcttcac tgcctgcctg
      721 gaaatatatc tacaaaaagc tgtgtaatac gcaatgcggc gcaggcctta actgacgatg
      781 gaactctata cggagccaca attcttggcg atggagttgt gcacaatagc ttcggtcaaa
      841 aactgatgcg catttacaat cagaaaggca tcttttcaaa cacaaaagat tccgaagaag
      901 gcttaacaca tatactctca gagcatttcg agaatgttaa aaccaaggtt caaggtactg
      961 tagtaatgtt ttccgcttca gggaaaaaat agcatccaac cgcagcacgt tcttgcataa
     1021 ggcgtgtctg cggtttttca actattcaga tacatcactc ccatcacatt cattcctccg
     1081 catcataggc atataggcta tatcaccttg atatttttct tcttcagata aaaactgtta
     1141 tctatgtata cttttaaacc caatccg
//
LOCUS       DAPHDW010000328         1152 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.336, whole genome shotgun sequence.
ACCESSION   DAPHDW010000328 DAPHDW010000000
VERSION     DAPHDW010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1152
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.336"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1050
                     /gene="ompC"
                     /locus_tag="RH057_004600"
     CDS             1..1050
                     /gene="ompC"
                     /locus_tag="RH057_004600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_063070036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HDV3130813.1"
                     /translation="MAMSAQAAEIYNKDSNKLDLYGKVNAKHYFSSNDADDGDTTYAR
                     LGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAFAGLKFGDYGSIDYGRN
                     YGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNNDFFGLVDGLNFAAQYQ
                     GKNDRSDFDNYTEGNGDGFGFSATYEYEGFGIGATYAKSDRTDTQVNAGKVLPEVFAS
                     GKNAEVWAAGLKYDANNIYLATTYSETQNMTVFADHFVANKAQNFEAVAQYQFDFGLR
                     PSVAYLQSKGKDLGVWGDQDLVKYVDVGATYYFNKNMSTFVDYKINLLDKNDFTKALG
                     VSTDDIVAVGLVYQF"
BASE COUNT          321 a          258 c          256 g          317 t
ORIGIN      
        1 atggcgatgt ctgctcaggc agctgaaatt tataataaag acagtaacaa gctggatctg
       61 tacgggaaag ttaatgctaa gcactacttc tcctctaatg atgcagatga tggtgatact
      121 acttatgccc gtcttggctt caaaggtgaa acccaaatca acgatcaact gactggtttc
      181 ggtcagtggg aatatgaatt caaaggcaac cgcgctgaat ctcaaggttc ctccaaagac
      241 aaaacccgtc ttgcatttgc aggcctgaaa ttcggtgact acggctcaat cgattacggc
      301 cgtaactacg gtgtagcata cgacatcggt gcgtggactg acgttctgcc agaattcggt
      361 ggcgatacct ggacccaaac agatgtgttc atgactggtc gcaccactgg tgttgcaacc
      421 tatcgtaaca acgacttctt tggtctggtt gatggtctga actttgctgc tcagtaccaa
      481 ggcaaaaacg atcgtagcga tttcgataac tacactgaag gtaacggtga tggcttcggt
      541 ttctctgcta cctatgaata cgaaggattc ggtatcggtg caacttatgc gaaatctgat
      601 cgtaccgaca ctcaagttaa tgcagggaaa gttcttcctg aagtatttgc ttccggtaaa
      661 aatgcagaag tttgggccgc aggtctgaaa tatgacgcta acaacattta cctggccact
      721 acctattctg aaacccagaa tatgactgta tttgctgatc acttcgttgc taataaagcc
      781 caaaacttcg aagctgttgc acaatatcag ttcgatttcg gtctgcgtcc gtccgttgct
      841 tacctgcaat ctaaaggtaa ggatcttgga gtatggggcg atcaggactt agtcaaatat
      901 gttgatgtag gtgcaaccta ttacttcaac aaaaatatgt ctactttcgt tgattacaaa
      961 atcaacctgc ttgacaaaaa tgacttcact aaagcactcg gtgtaagcac tgatgacatc
     1021 gttgctgtag gtctggttta ccagttctaa tctgattacg aaaaagatat gttgcgggag
     1081 gcgttgcctc cccaacatat aagtggctcc ctcaagccac ttcctttaga agcacaacct
     1141 tgcttctaac ta
//
LOCUS       DAPHDW010000329         1141 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.337, whole genome shotgun sequence.
ACCESSION   DAPHDW010000329 DAPHDW010000000
VERSION     DAPHDW010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.337"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..706
                     /locus_tag="RH057_004601"
     CDS             32..706
                     /locus_tag="RH057_004601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001404119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3130814.1"
                     /translation="MLNEENPHQLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYIT
                     QDPIGLKGGWNFYQYPLNPVQYIDSMGLASKYGHLNNGGYGARPNKPPTPDPSKLPDI
                     AKQLRLPYPIDQASSAPNVFKTFFRALSPYDYTLYCRKWVKPNLTCTPQDDSQYPGMD
                     TKTASDYLPQTNWPTTQLPPGYTCAEPYLFPDINKPDGPATAGIDDLGEILAKMKQRT
                     SRGIRK"
     gene            703..>1141
                     /locus_tag="RH057_004602"
     CDS             703..>1141
                     /locus_tag="RH057_004602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130815.1"
                     /translation="MKRVLFFLLMIFVSFGVIADCEIQAKDHDCFTIFAKGTIFSAFP
                     VLNNKAMWRWYQNEDIGEYYWQTELGTCKNNKFTPSGARLLIRVGSLRLNENHAIKGT
                     LQELINTAEKTAFLGDRFRSYIRAGIYQKKSSDPVQLLAVLDNS"
BASE COUNT          361 a          226 c          268 g          286 t
ORIGIN      
        1 atggtgcgca gaatacgatg aatggggcaa cctgctgaat gaagagaacc cgcatcagct
       61 gcagcagctt atccgcctgc cggggcagca gtatgatgag gagtccggcc tgtattacaa
      121 ccgccaccgc tattatgacc cgctgcaggg gaggtatatc actcaggatc cgattgggct
      181 gaagggggga tggaattttt atcagtatcc gctgaatccg gttcagtata tagattcaat
      241 gggactggca tcaaaatatg gacacttaaa taatggcgga tatggagcga gacccaacaa
      301 accgcctacg cccgatccaa gtaaattgcc ggacatagcg aaacaattaa gactgccata
      361 tcctattgac caggccagta gtgcgcctaa tgttttcaaa acattcttca gagcattaag
      421 cccttacgac tacacactgt attgcaggaa gtgggtaaaa ccaaatctga cttgtacgcc
      481 acaggatgat tcccagtatc cagggatgga tacaaagaca gcaagtgatt acctgccaca
      541 gacaaattgg ccaacaactc aattaccacc aggatatact tgtgcagaac cctatttatt
      601 cccagacatt aataaacccg atgggccagc aacagcaggg atagatgatt tgggtgaaat
      661 tttagctaag atgaaacaga gaacatcgag aggaataaga aaatgaaaag agttttgttc
      721 tttttgctga tgatatttgt tagttttggt gttatagctg attgcgaaat acaagctaaa
      781 gatcatgatt gttttactat tttcgctaag ggtactattt tttccgcgtt tcctgtctta
      841 aataataaag ccatgtggag atggtatcag aatgaggaca tcggtgagta ttattggcaa
      901 acagaactgg gtacatgtaa aaataataaa ttcacaccaa gcggggcgag actcttaatt
      961 cgcgtcgggt cactacgcct taatgaaaat catgccatca aaggaacgtt gcaggagttg
     1021 ataaatacag cagaaaaaac ggcgtttctc ggcgatcggt ttagaagtta tataagagcg
     1081 ggtatctatc aaaaaaagag cagtgatcca gttcagttgt tggcagtcct tgataattca
     1141 a
//
LOCUS       DAPHDW010000330         1107 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.338, whole genome shotgun sequence.
ACCESSION   DAPHDW010000330 DAPHDW010000000
VERSION     DAPHDW010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.338"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..1107
                     /locus_tag="RH057_004603"
                     /pseudo
     CDS             1..1107
                     /locus_tag="RH057_004603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080172.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66-like element ISEc43 family transposase"
BASE COUNT          277 a          276 c          333 g          221 t
ORIGIN      
        1 accgcctgga gcctgaagaa agctgttgcc cggagtgtgg cggtgagctt gattatctgg
       61 gggaagtcag cgcagaacaa ctggaactgg tgagcagcgc cctgaaagtg atccgcacag
      121 aacgggtaaa aaaagcctgt acaaaatgtg actgcatcgt tgaagcaccg gcaccatccc
      181 gtccgataga gcgtggtatc gcgggcccgg ggttacttgc ccgcgtgtta acgggaaaat
      241 actgcgaaca cctgccactg tatcgtcaga gtgaaatctt tgcccgccag ggtgtcgaac
      301 tgagccgtgc cttactctcc aactgggttg atgcgtgctg ccagttaatg acgccgctga
      361 atgatgccct gtacagttat gtgatgaaca cccgcaaggt tcacactgat gacacaccag
      421 taaaagtact ggcaccgggc aggaagaagg cgaaaacagg atatatctgg acgtatgtcc
      481 gggatgaccg aaatgccggt tcgccagaac ctccggcggt ctggttcgcc tactcaccgg
      541 accatcaggg taaacatccg gagcaacacc ttcgtccctt ccgggtatcc tgcaggcaga
      601 tgcgttcgca ggttacgatc ggctgttcag tgccgaacgt gaaggcggcg cgttgacgga
      661 agcaggatgc tgggctcatg cgcggcgcaa aatccacgat gtatatatca gtaccaaaag
      721 cgcgacggcg gaagaagcac tgaaactaat cggcgaactg tacgccattg agcacgaaat
      781 acgcgggttg ccggtgtctg aacgcctggc ggtcaggcaa atgcagagta aaccgctact
      841 gacttccctg tataagctga tgcaggagaa agaacacacg ttatcgaaaa aatgccgtct
      901 gagagatgcg ttccggtata tcaggaagca ctgggttgcg ttgtgcaact tctgtgatga
      961 cggtctggcg gaggcggaca ataacacagc ggaaagagcg cttcgtgcag tctgtctcgg
     1021 aaagaaaaat tacgtgttct tcggtagcga tcacggcggc gagcgtggtg cactgttgta
     1081 cgggctgatc ggcacctgcc ggttgaa
//
LOCUS       DAPHDW010000331         1105 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.339, whole genome shotgun sequence.
ACCESSION   DAPHDW010000331 DAPHDW010000000
VERSION     DAPHDW010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1105
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.339"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            43..204
                     /locus_tag="RH057_004604"
     CDS             43..204
                     /locus_tag="RH057_004604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001593559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HDV3130816.1"
                     /translation="MMQNQTEEAKRILKTDGYSGSNPVLSRLMEEVRNEKNTTVGSRY
                     DRVHNRHNR"
     gene            211..>1105
                     /locus_tag="RH057_004605"
     CDS             211..>1105
                     /locus_tag="RH057_004605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HDV3130817.1"
                     /translation="MLDTILIKTASRCNLDCTYCYVYRGADTSWQDQPYRMNDATIEK
                     VVERITEYSLLQETGFAIVLHGGEPLLLGERRLESLLSGLRRVLNPQKYPISIQTNGV
                     LITEKILNLCSKYRVSISVSIDGTRKANDIARFTRGGNSSFEDVINGIKKLSSHQDHA
                     FLFAGTLSVIQPNIPAEDTYHYLKSINSPSIDFLFQDGNYDRMPYGKKDLQTTEYGEW
                     LAKVFELYMEDSNPVPIKILDDILKIYLGGSPIKEGKGNNEFGILIIESDGEIRKNDT
                     LRASFEGVDFFKGRPSVHNTSL"
BASE COUNT          386 a          159 c          237 g          323 t
ORIGIN      
        1 cttattattg tactattttg ataacaattc aatgaggata gtatgatgca aaaccaaacg
       61 gaagaagcaa aaagaatctt aaaaacagat ggttacagcg gatctaaccc ggtgttatct
      121 agactgatgg aagaagttag aaatgaaaaa aacacaacgg taggtagccg ttatgatcga
      181 gtccataatc gccataaccg ttaaatttct atgcttgata caattttaat caagactgcc
      241 agtaggtgca atttggattg cacctactgc tatgtatatc gcggggctga tacaagctgg
      301 caggatcaac cctataggat gaatgatgcc acgatagaaa aagtggtgga aaggattacg
      361 gaatatagtc ttttacagga aactggattt gccatagttt tacatggagg tgaacctctt
      421 cttttggggg aaagaagact tgagagttta ttgtcaggtt taaggagagt tttaaatcct
      481 caaaaatacc cgataagtat acaaaccaat ggtgttttga taactgagaa aattttgaac
      541 ttatgttcaa aatatagagt aagcatttcg gttagcattg acggcacaag aaaagctaat
      601 gatatcgcga ggtttacacg tggtggtaac agtagttttg aggatgtaat taacggaatt
      661 aaaaaattaa gttcacacca agaccacgcc ttcttgtttg cagggacatt atcagtaata
      721 caaccgaata ttcctgctga ggatacatat cattacctga agagtataaa ctctccaagt
      781 atagattttc tatttcagga tggaaattat gataggatgc cttatggaaa aaaagatctt
      841 caaactactg aatacggaga gtggttagct aaagtatttg aactctatat ggaagatagc
      901 aatcctgttc cgataaaaat actggatgat attctcaaga tttatttggg tggttctcca
      961 ataaaagaag ggaaaggaaa taatgagttt ggtattttga taattgaatc tgatggagag
     1021 attagaaaga atgatacatt gagagcatca tttgaaggtg tcgatttttt caaaggacgt
     1081 ccgtctgttc ataatacttc attaa
//
LOCUS       DAPHDW010000332         1085 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.340, whole genome shotgun sequence.
ACCESSION   DAPHDW010000332 DAPHDW010000000
VERSION     DAPHDW010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1085
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.340"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..617
                     /locus_tag="RH057_004606"
     CDS             <1..617
                     /locus_tag="RH057_004606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HDV3130818.1"
                     /translation="GCQKDIAEKIQKQGGDYLFAVKGNQGRLNKAFEEKFPLKELNNP
                     EHDSYAMSEKSHGREEIRLHIVCDVPDELIDFTFEWKGLKKLCVAVSFRSIIAEQKKE
                     PEMTVRYYISSADLTAEKFATAIRNHWHVENKLHWRLDVVMNEDDCRIRRGNAAELFS
                     GIRHIAINILTNDKVFKAGLRRKMRKAAMDRNYLASVLAGSGLS"
     gene            647..>1085
                     /locus_tag="RH057_004607"
     CDS             647..>1085
                     /locus_tag="RH057_004607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016247180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130819.1"
                     /translation="MLVGAAVGAVVIGGGIVIYNNREAVGDAIEKSMEMRGEAELLNA
                     QSQMEFWRDPYGAFSSVFGNDDSSKENPNVGKDLTDEEKNCLGSGSTGGSGGWEPDED
                     GEIRNTYRSIKDAPQYPRGVRNVQNGTTRNVVKDQQLLKQLRKV"
BASE COUNT          347 a          173 c          311 g          254 t
ORIGIN      
        1 tgggctgcca gaaagatatt gcagaaaaga tacaaaaaca gggaggtgat tatttattcg
       61 cggtaaaagg aaaccagggg cggctaaata aagcctttga ggaaaaattt ccgctgaaag
      121 aattaaataa tccagagcat gacagttacg caatgagtga aaagagtcac ggcagagaag
      181 aaatccgtct tcatattgtt tgcgatgtcc ctgatgaact tattgatttc acgtttgaat
      241 ggaaagggct gaaaaaatta tgcgtggcag tctcctttcg gtcaataata gcagaacaaa
      301 agaaagagcc cgaaatgacg gtcagatatt atatcagttc tgctgattta accgcagaga
      361 agttcgccac agcaatccga aaccactggc acgtggagaa taagctgcac tggcgtctgg
      421 acgtggtaat gaatgaagac gactgcagaa taagaagagg aaatgcagcg gaattatttt
      481 cagggatacg gcacattgct attaatattt tgacgaatga taaggtattc aaggcagggt
      541 taagacgtaa gatgcgaaaa gcagccatgg acaggaacta tctggcgtca gtccttgcgg
      601 ggagcgggct ttcgtaatct tgccctgggt aggaggccct ccagggatgt tagtcggtgc
      661 ggcagttgga gcggtggtta ttggtggtgg tatagtaata tacaacaatc gcgaggctgt
      721 tggggatgcg attgagaaaa gtatggagat gcggggtgaa gccgaattat taaatgcaca
      781 aagtcaaatg gaattctggc gagatcccta tggtgctttt tccagcgtgt ttggaaatga
      841 tgattcatca aaagagaatc caaatgtcgg gaaggatttg acggatgaag agaagaactg
      901 tttgggcagt ggcagtacag gaggttcggg agggtgggag ccagatgagg atggcgaaat
      961 aagaaatacc tacaggtcta ttaaggatgc tcctcagtat cccagagggg ttaggaatgt
     1021 ccaaaatggc actacaagga atgttgtaaa ggatcagcag ttattgaaac aattacgtaa
     1081 agtag
//
LOCUS       DAPHDW010000333         1082 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.341, whole genome shotgun sequence.
ACCESSION   DAPHDW010000333 DAPHDW010000000
VERSION     DAPHDW010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1082)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1082
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.341"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..1054
                     /locus_tag="RH057_004608"
     CDS             <1..1054
                     /locus_tag="RH057_004608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HDV3130820.1"
                     /translation="LIFISLSVNAAHTGATFSQKGGEMSNQASPDGWTISQEGRVGAG
                     NATDKRISIGTESEYTNKSVGYDPFTGKDIDGSQNATISIGNSNYKRQIKNVENGIDD
                     YDAVNVSQLKAVSNSASEAISIVNNANTSINQAVNIANSLNDTVSKGINIGADNGPVV
                     KRELGDTVTVTGDSNIRTKTTSTGVKVALNQNINLGENGSVNTGNTTVNSHGVIINEG
                     PSMTRSGFNAANNRITNVAPGINNTDAVNVGQLNNVVSNVGKSIQNLNNRINHVEKNA
                     NAGIAEAMATAGLPQAYLPGKSMMAIGGGSYSGETGYAIGYSTISDNGRWMFKGTGSG
                     NSRGNYGGTLGVGYQW"
BASE COUNT          360 a          168 c          258 g          296 t
ORIGIN      
        1 tttaatcttc atttcattat ctgtaaatgc tgcacatact ggagcaacct tttctcaaaa
       61 aggaggagaa atgtcaaatc aggcctcgcc ggatggctgg actatatctc aggaaggacg
      121 tgttggagca ggtaatgcta cagataaaag aatttccatt ggtacagaaa gtgaatatac
      181 taataaaagt gtagggtatg accctttcac aggcaaagat atcgatggta gccagaatgc
      241 aacaataagt attggtaata gtaattacaa acggcagata aaaaatgtag agaatggtat
      301 tgatgattat gatgctgtta acgtttctca gttaaaggct gtcagtaata gtgcatctga
      361 ggctattagc attgtcaata atgctaatac tagtataaac caggcggtta atatagctaa
      421 cagtttaaac gatacggtca gtaaaggaat aaatataggt gctgataatg gtcctgtagt
      481 aaaacgagaa ttaggagata cagttactgt aaccggagac agtaatataa gaaccaaaac
      541 caccagtacc ggggtcaaag tcgctttaaa tcagaatatt aatcttggtg agaatggtag
      601 cgtgaatact ggtaacacaa ccgttaattc tcatggtgta attattaatg aaggcccgag
      661 catgacgcgg agtggcttta acgctgctaa taatagaatc accaatgtag ctcctgggat
      721 aaataataca gatgcagtta atgtgggaca gctaaataat gtagtatcta atgtagggaa
      781 gagtattcaa aatttgaata accgcatcaa ccatgtggag aaaaatgcaa atgcaggcat
      841 agctgaggct atggccacag ccggactgcc gcaggcttat ctgcctggta agagtatgat
      901 ggcgataggt ggcggttctt atagtggtga aacaggctat gcaattggct attccaccat
      961 atcggataat ggcagatgga tgtttaaagg tactggcagt ggtaattcac gtggtaacta
     1021 tggtggaacc cttggtgttg gttatcagtg gtaatactat attcttggtg tacattttta
     1081 ta
//
LOCUS       DAPHDW010000334         1067 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.342, whole genome shotgun sequence.
ACCESSION   DAPHDW010000334 DAPHDW010000000
VERSION     DAPHDW010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.342"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..214)
                     /locus_tag="RH057_004609"
     CDS             complement(<1..214)
                     /locus_tag="RH057_004609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3130821.1"
                     /translation="MKACLLLFFYFSLIGQLHGADVKIKENESVMGSTAMTYDLSEEK
                     LMKLKYKSQHGDSEASFRLYQYYCFTK"
     gene            complement(226..>1067)
                     /locus_tag="RH057_004610"
     CDS             complement(226..>1067)
                     /locus_tag="RH057_004610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3130822.1"
                     /translation="NQMDPVYTPARKIHLYHCDHRGLPLALISTEGATAWCAEYDEWG
                     NLLSDENPHHLQQLIRLPGQQYDEESGLYYNRHRYYDPLQGRYITQDPIGLKGGWNFY
                     QYPLNPVINVDPQGLVDINLYPESDLIHSVADEINIPGVFTIGGHGTPTSIESATRSI
                     MTAKDLAYLIKFDGNYKDGMTVWLFSCNTGKGQNSFASQLAKELHTNVIGPDTLWTWW
                     GRGTNGKLKMDTVLTAPTNLNSNKDLMAITTKDLGNWITYGPSGHPISNMQGTPEKPS
                     DIR"
BASE COUNT          275 a          251 c          209 g          332 t
ORIGIN      
        1 ttttagtaaa gcagtaatat tgatatagtc gaaatgatgc ctctgaatca ccatgttgtg
       61 atttatactt cagtttcatc agcttttctt cacttaaatc ataggtcatt gctgtagatc
      121 ccatcacact ttcgttttct tttattttca catcagcacc atgcaattga ccaataagag
      181 aaaaataaaa aaatagtaac aagcatgctt tcatacatct acaacctatc ttatatcact
      241 gggtttttct ggcgtacctt gcatattaga aatggggtgc ccagatggcc catatgttat
      301 ccaattacca aggtcttttg ttgttatagc cattagatcc ttatttgaat taaggttcgt
      361 tggtgctgtt agcactgtat ccatttttaa cttaccatta gttcctcgcc cccaccacgt
      421 ccatagcgtg tcaggtccta ttacatttgt atgtaactct ttagctaatt ggctagcaaa
      481 tgaattttgt cctttacctg tattacaaga aaataaccaa actgtcatcc catctttata
      541 attcccatca aatttaatta gatatgctag atctttagct gtcatgatac tgcgcgttgc
      601 ggattcaata gatgtggggg taccatgccc cccgattgtg aaaacgcctg ggatattaat
      661 ctcatcagct acagaatgga taagatcact ttcggggtat aaatttatat caaccaaacc
      721 ttgcggatct acatttatga ccggattcaa cggatactga taaaaattcc atcccccctt
      781 cagcccaatc ggatcctgag tgatataccg cccctgcagc gggtcataat agcggtggcg
      841 gttgtaatac aggccggact cctcatcata ctgctgaccc ggcaggcgaa tgagctgctg
      901 cagatgatgc gggttctcat cactcagcag gttgccccat tcatcatatt ctgcgcacca
      961 cgctgttgcc ccttccgtgc tgatgagcgc cagcggcagg ccgcgatggt cgcagtggta
     1021 caggtggatt tttcgcgccg gcgtgtacac cgggtccatc tggtttt
//
LOCUS       DAPHDW010000335         1066 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.343, whole genome shotgun sequence.
ACCESSION   DAPHDW010000335 DAPHDW010000000
VERSION     DAPHDW010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1066
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.343"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1066
                     /gene="gltS"
                     /locus_tag="RH057_004611"
     CDS             <1..>1066
                     /gene="gltS"
                     /locus_tag="RH057_004611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HDV3130823.1"
                     /translation="LIGMFVVKFTPFLQKNHIPEAVVGGFIVAIVLLIIDKTSGYSFT
                     FDASLQSLLMLTFFSSIGLSSDFSRLIKGGKPLVLLTIAVTILIAIQNTVGMSMAVMM
                     NESPFIGLIAGSITLTGGHGNAGAWGPILADKYGVTGAVELAMACATLGLVLGGLVGG
                     PVARHLLKKVSIPKTTEQERDTIVEAFEQPSVKRKINANNVIETISMLIICIVVGGYI
                     SALFKDTFLQLPTFVWCLFVGIIIRNTLTHVFKHEVFEPTVDVLGSVALSLFLAMALM
                     SLKFGQLASMAGPVLIIIAVQTVVMVLFACFVTFKMMGKDYDAVVISAGHCGFGMGAT
                     PTAIANMQTVTKAFGPSHKAF"
BASE COUNT          285 a          201 c          229 g          351 t
ORIGIN      
        1 actcatagga atgtttgtcg taaaatttac cccgttccta caaaaaaacc acataccaga
       61 agccgtcgtt ggtggcttta ttgttgcaat tgttctgtta attattgata aaacatcagg
      121 ttattcgttt acttttgatg cttcattgca aagtttatta atgctcacat tcttttcctc
      181 tatcgggcta agttctgact tttctcgact gattaaagga ggaaagccgt tagttctatt
      241 aactattgca gtaacgatcc taatcgccat tcaaaatact gtcggcatga gtatggctgt
      301 catgatgaat gaaagtccat ttattggctt aattgcaggt tcaattactc taacaggtgg
      361 tcatggtaat gccggagcat ggggccctat tctcgctgat aaatatggtg taacaggcgc
      421 cgttgaatta gcgatggctt gtgcaacact tggattagtg ttgggtggtt tagttggcgg
      481 ccctgttgcc cgtcatcttt tgaaaaaggt ctctattcct aaaacaaccg agcaagagcg
      541 cgacactatc gttgaagctt ttgagcaacc aagcgtcaaa agaaaaatca atgcaaataa
      601 cgttattgaa accatttcaa tgctgattat ctgtattgtt gttggtggct atatcagtgc
      661 attgtttaaa gatacttttc tgcaactgcc tacttttgtc tggtgtttat ttgtcggtat
      721 tattatccgt aatacactga ctcatgtatt taaacacgaa gtgtttgagc cgaccgtcga
      781 tgtattaggt agcgttgctt tatcgctttt cttggcaatg gcgttaatgt cattaaaatt
      841 tggtcaattg gcaagcatgg cagggccagt attaattatc attgctgtac aaactgttgt
      901 catggtgcta tttgcctgct ttgtcacctt caaaatgatg ggcaaagatt atgatgctgt
      961 cgtgatcagc gcgggtcact gtggctttgg tatgggagca acaccaacag caattgcgaa
     1021 tatgcaaaca gtcacaaaag catttggacc atcacataaa gctttc
//
LOCUS       DAPHDW010000336         1039 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.344, whole genome shotgun sequence.
ACCESSION   DAPHDW010000336 DAPHDW010000000
VERSION     DAPHDW010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1039)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.344"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          396 a          236 c          152 g          255 t
ORIGIN      
        1 actcagggga aactcatcac aaggctgacc gaatgacata gtaacctcac tatacacaga
       61 tataaaagca ctgcaattct ggctattaat agcaaaaata gcagtcaaat caatatgagg
      121 taaaaatttt tgttgattta atggttaaat aataaccaca gtgtgttttg ctgctgaaga
      181 aagacaaaat gaatctgtgt gtcccctcct tcccttcaga gcacatccac tattcaaatt
      241 ttacacaacc acgtacgaac aaaaagcatc atcccgggta ttgcagatac cggtttcctg
      301 ccgggaagaa tcataaacag aacaccggga ataacgcctt caggaatcac cccccctcct
      361 gaagaagaat aaaataaaga agactacagt tcctgcactg aatgctgagt acaatattct
      421 atggacagga tgacattttt ataaataaca aaaaccattt aatactgata catcaataaa
      481 atacttcttc cctgctgaca cagataaaat gcaccatttt tccttcaaat acttctgtat
      541 aatcgccagc caatgatgag aatggattaa aataacactt cttcgacaaa cacaaaaaca
      601 gccttcattt tacatcatca tccagtttca gttaaccccg gacgagcgac agcgtttcag
      661 tacaggaatt aaaagccagc ttttgctcag taaaaacaca ctcaactaat aatatctcag
      721 cagatattat cctgaccagg acacctcaca ttactgaatt ctcttccgac agtatcactg
      781 cacaaataaa aaagaacggg ataaatattc agaccggaag aatggtgtca caaaagcatt
      841 taatcattac acatgaccag gaagggaaac acttaaccac atggtatcaa cagatatcat
      901 tacgtgtgca ctcttataaa aaacacacag aagaagtaag ataattataa atgtaaattt
      961 aatatctccc actgacatct cccccacaat acactgaaaa atactcacag cagtaaacac
     1021 agacacacct gataatcat
//
LOCUS       DAPHDW010000337         1029 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.345, whole genome shotgun sequence.
ACCESSION   DAPHDW010000337 DAPHDW010000000
VERSION     DAPHDW010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1029
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.345"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..282)
                     /locus_tag="RH057_004612"
     CDS             complement(<1..282)
                     /locus_tag="RH057_004612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130824.1"
                     /translation="MKPRNINNSLPLQPLVPDQENKNKKNEEKSVNPVKITMGSGLNY
                     IEQESLGGKYLTHDLSIKIADISEEIIQPAILSAMSIYKFSITDDLMSMA"
     gene            476..901
                     /locus_tag="RH057_004613"
     CDS             476..901
                     /locus_tag="RH057_004613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311733.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130825.1"
                     /translation="MDIEFSQIDEMVYMHDIVNSDSKKKPRIPLKKFLNAEKVLTQTT
                     SWALNSRFVNVNSVNKVNVKSKVKSSYISRSVNDEFSLTDDEINSFKETLVLSSIDSL
                     SKLVLNNPLSVLFTSTVRRNNNRAKMNVEFDSWICTRCC"
     gene            937..1017
                     /locus_tag="RH057_004614"
     CDS             937..1017
                     /locus_tag="RH057_004614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130826.1"
                     /translation="MKDVDQNFDALDCHILREYLILLFYD"
BASE COUNT          327 a          155 c          182 g          365 t
ORIGIN      
        1 agccatactc attaaatcat ctgttatcga aaatttatat atgctcatag cagataatat
       61 tgctggctga attatctctt cagaaatatc cgctatcttt attgacaaat catgtgttag
      121 atattttcct ccaagagatt cttgttcaat ataatttaaa ccagacccca ttgtgatttt
      181 aactggatta acggatttct cttcattttt cttatttttg ttctcctgat caggaactaa
      241 tggttgcagt ggtaggctat tattaatatt tcgtggcttc attttacatt tctctcattt
      301 gattagagca aaatccgaat tcgcagcttc tgataatgtc aaatatcacc ttatggagac
      361 gttacgtaga atgactatat ttgttatcgg cacattaggg gtattaattt tagtattgat
      421 ggaaggtgat tattgaacag taacatcgag tcacggtaaa aatgtgaggt atataatgga
      481 cattgagttt tcgcagattg atgaaatggt ctatatgcat gatattgtaa attcggactc
      541 gaaaaagaaa ccaaggattc cgttaaaaaa attccttaat gctgagaagg ttctcacgca
      601 aacaacatca tgggcattaa attcaagatt tgtgaatgtt aattctgtca ataaagtcaa
      661 cgtaaagagt aaagttaaaa gcagttatat ctcacgatct gtcaatgatg agtttagttt
      721 aacagatgat gaaatcaatt cttttaaaga aacgttagtc ctttcctcta ttgattcact
      781 gagtaaactg gttcttaata atcctctttc tgtattgttt acatcgactg tacgaaggaa
      841 taataatcga gctaagatga atgtcgagtt cgactcatgg atttgcacca gatgttgttg
      901 atacatgata ttttacattg caaggagatg tctaaaatga aagatgttga tcaaaacttt
      961 gatgctttag actgccatat actgcgagaa tatttaattt tattgtttta tgattaacga
     1021 ggttccatc
//
LOCUS       DAPHDW010000338         1028 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.346, whole genome shotgun sequence.
ACCESSION   DAPHDW010000338 DAPHDW010000000
VERSION     DAPHDW010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1028
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.346"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            116..>1028
                     /gene="tdcA"
                     /locus_tag="RH057_004615"
     CDS             116..>1028
                     /gene="tdcA"
                     /locus_tag="RH057_004615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HDV3130827.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCR"
BASE COUNT          285 a          196 c          237 g          310 t
ORIGIN      
        1 gcgtaacgat accatggaaa gtattgaaag ttaatttgtg agtggtcgca catatcctgt
       61 tcatttcatt ttgatacact tcatgccgtc aatgaggtaa ttaacgtagg tcgttatgag
      121 cactattctt cttccgaaaa cgcagcacct ggtagtcttt caggaagtca ttagaagtgg
      181 ctctatcggc tcggctgcaa aagaattagg gttaactcaa ccggccgtca gtaaaatcat
      241 taacgatatt gaagattatt ttggtgtgga attagtggtg cggaaaaata ccggtgtaac
      301 attaacacct gccggtcaat tgttactctc ccgttccgaa tccattaccc gtgaaatgaa
      361 aaatatggtt aatgagataa gcggtatgtc ttctgaggcg gtggtggaag tctcatttgg
      421 ttttccttca ttgattggtt ttacttttat gtcagggatg atcaacaagt tcaaagaggt
      481 gttcccgaaa gcgcaggttt ctatgtatga agcgcaactg tcttcgttct taccggcaat
      541 ccgcgacggt cgactggatt ttgcgattgg tacgttaagt gcagaaatga agcttcagga
      601 tttacatgtt gagccgctgt tcgagtccga gtttgtgctg gtagccagta agtcccgaac
      661 atgcaccggc accaccacgc tggagtcgtt gaagaacgaa cagtgggtgt tgccacaaac
      721 caatatgggg tactacagcg agcttcttac tacgttacaa agaaatggca tcagtattga
      781 aaacatcgtt aaaaccgact cagtcgtgac aatttataat cttgttctca atgctgattt
      841 cttaactgta attccttgtg atatgacgtc accttttggt tcaaatcaat ttattactat
      901 tccggttgaa gaaacattac ctgtggcaca atatgccgcg gtatggtcga aaaattatcg
      961 tattaaaaaa gcagcatcgg ttttggtgga attagccaaa gagtattcat cttataatgg
     1021 gtgtagac
//
LOCUS       DAPHDW010000339         1026 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.347, whole genome shotgun sequence.
ACCESSION   DAPHDW010000339 DAPHDW010000000
VERSION     DAPHDW010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.347"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1026
                     /locus_tag="RH057_004616"
     CDS             <1..>1026
                     /locus_tag="RH057_004616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HDV3130828.1"
                     /translation="LAGRPMMDTLVNQHSDINKTHEILQDESKCEMIVIIENFMTSSA
                     KYADILLPDLMTVEQEDIIPNDYAGNMGYLIFLQPVTSEKFERKPIYWILSEVAKRLG
                     PDVYQKFTEGRTQEQWLQHLYAKMLAKDPALPSYDELKKMGIYKRKDPNGHFVAYKAF
                     RDDPEANPLKTPSGKIEIYSSRLAEIARTWELEKDEVISPLPVYASTFEGWNSPERRT
                     FPLQLFGFHYKSRTHSTYGNIDLLKAACRQEVWINPIDAQKRGIANGDMVRVFNHRGE
                     VRLPAKVTPRILPGVSAMGQGAWHEANMSGDKIDHGGCVNTLTTLRPSPLAKGNPQHT
                     NLVEIEK"
BASE COUNT          292 a          263 c          238 g          233 t
ORIGIN      
        1 cttggctggt cggcccatga tggataccct cgttaatcag cattctgaca tcaacaaaac
       61 gcatgaaatt ctgcaggacg aatcgaaatg cgaaatgatt gtgatcatcg aaaactttat
      121 gacctcatcg gcaaaatatg ccgacattct gctgccagac ctgatgaccg ttgagcagga
      181 agatattatt cctaacgact acgccggtaa catgggatat ctcattttcc tccagcctgt
      241 caccagcgaa aaattcgaac gcaaaccgat ttactggatc ctgagtgaag tcgcgaaacg
      301 tcttggacct gacgtctatc aaaagttcac agaaggtcgc acgcaggaac aatggttaca
      361 acatctgtac gccaaaatgc ttgccaaaga tccggcgtta ccgtcttacg acgaactgaa
      421 aaaaatgggt atctataagc gtaaagatcc caatggccat tttgtcgcct acaaagcatt
      481 tcgtgacgac cccgaggcaa atccacttaa aacgccttcc ggtaagattg aaatttattc
      541 cagcaggctg gcggaaattg cccgtacctg ggaactggaa aaagatgaag tgataagccc
      601 attgccagtt tatgcctcaa cctttgaagg ctggaactcc cctgaacgta gaaccttccc
      661 actgcaactg tttggtttcc attacaaatc ccgtactcac tcgacctacg gcaatattga
      721 tctcctgaag gctgcctgcc gtcaggaggt gtggatcaac cctatagatg cgcagaaacg
      781 tgggattgcc aacggcgata tggtgcgggt gtttaaccat cgtggcgaag ttcggctacc
      841 agccaaagta acaccacgta ttctccctgg agttagcgct atgggccagg gagcctggca
      901 cgaggccaat atgtctggcg ataaaatcga ccatggcggc tgtgtgaata cgctaaccac
      961 tctgcgccct tcaccactgg cgaagggaaa cccgcagcac actaatctgg tcgagatcga
     1021 aaaaat
//
LOCUS       DAPHDW010000340         1026 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.348, whole genome shotgun sequence.
ACCESSION   DAPHDW010000340 DAPHDW010000000
VERSION     DAPHDW010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1026
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.348"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            229..771
                     /locus_tag="RH057_004617"
                     /pseudo
     CDS             229..771
                     /locus_tag="RH057_004617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          292 a          194 c          255 g          285 t
ORIGIN      
        1 taaatcccag attacttaca gaaatgaatg gaatgaggaa tttaaatcca gagtttcagt
       61 cagaagtaat gatattatgc ttggcgtgac atatgatttc tgacatacca tattgaaata
      121 ttacattagt ttatcaaaat gttataaaat taccggatgt tctgggagtg gaacatccgg
      181 tttgtgccta atgagggaat tgtggcactc cgttactgtt ccagtcgaat tgcggataat
      241 actcaactaa tcagtctaac ctgcggttgt tcacaatcac cgacaccgta aatggcgtcc
      301 ccctgatatc tgccagttca tgacattagt cgaagtcggt gttgcgttat gtgccaggag
      361 catggttgtc gggagactgc ctgtcccgtg ggctggttcc ggcaaccggt atactctgtg
      421 gttcgaattg ttcgtgctct catggttaaa aatcagtact gttgaaggcg tcaaaaacca
      481 gttggaatgc tgttgacggc attatgacac gtgcggttaa gtgcggcctg tcccggataa
      541 tccgtatctt cctggtgaaa gtcgttatca ggaaaaggga atttgcttcc ggtggcagta
      601 cagcgataag tggatgacca aatccaggca ggaaaagctg atatggctgc gtacgcagat
      661 gaagctgacg agccatttcc tggcgcgatg aaagaactgg caaaggatat caggagcaga
      721 ccatgtatcg aggaaagacg caatgactgg cagagatgga tggcgttggc ggtcaacatg
      781 gaaaatccat gaatgtgaaa tttattattg tcatggttat aatgactata ttaacggggt
      841 atgctttacc tgccatacct gaacggttaa agatatgatt gctgccgttc cggtttatca
      901 gttgaaatac agaatcctat tctataatgc tctgagaatg aaaacatctg cgtatacaat
      961 tacagaatgt gcactgacgc ctgcggagga aatattagag gacttgacat ctcattatta
     1021 tatccg
//
LOCUS       DAPHDW010000341         1022 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.349, whole genome shotgun sequence.
ACCESSION   DAPHDW010000341 DAPHDW010000000
VERSION     DAPHDW010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1022)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1022
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.349"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..213)
                     /locus_tag="RH057_004618"
     CDS             complement(<1..213)
                     /locus_tag="RH057_004618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130829.1"
                     /translation="MADKAILWALISASNKEGRKACSLSYFACKAAEAELGLAYMAAN
                     DNKEFLTSLSNIMRYKIDAGLSESYTC"
     gene            complement(920..>1022)
                     /locus_tag="RH057_004619"
     CDS             complement(920..>1022)
                     /locus_tag="RH057_004619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HDV3130830.1"
                     /translation="EFAVNNGNIDTKSKLQELKKLKRMDRRKNKENP"
BASE COUNT          295 a          220 c          176 g          331 t
ORIGIN      
        1 gcatgtatag gattcggaaa gtccagcatc tattttgtac ctcattatat tagataagga
       61 tgtgagaaat tccttgttat cattcgcagc catatatgcc agtccgagtt cagcctctgc
      121 ggccttacag gcaaaatagc ttagtgaaca cgcttttcgt ccctctttat tactggctga
      181 tattagtgcc cataatattg ctttgtctgc caaatctaac tctctctgaa attgtcttgc
      241 agtaactgca ctcgcagaaa tagagaaaaa aatacaattg ttaataacct tgccatattt
      301 tttagtccgt aactgttaat gtgctatctg gctctgaaaa tattaactca tttaattttt
      361 gcatgtcatt agataaacca gttatacaac cctcggaaca gaatcgaggt cctaaagacg
      421 gaccaaaagc gtttaaacaa ctatgtattg aaaatccatt gcgattataa gttcctgtat
      481 taactgacgg tactaatcgt atgccgtcag gttttctatt gcttgctccc gggtcattaa
      541 cattccaact ccataggcct tgaggtattg gccatctatt tataaatgtg tacaacgggg
      601 attattttta cattgcatat tattaccaga agcgactgga atactaagca catcataaaa
      661 attatcatca catggtatat ctgaaacaca cgaaagcata cctaaagtta tactgtataa
      721 acaggtggct aatcccaagg ggatcgattc cagaaatcgg gttcaacgga tactgataaa
      781 aattccacct accttccagc ccaatcggag cctgagtgtt ataccgcccc tgctgcggtt
      841 aatgttaccg ataatagtta tagtacagcc ctgattcatc atcatactac tgctgcggca
      901 gtctgatgaa ctattgcttt tacggattct ccttattctt ccttctatcc attcgcttta
      961 gctttttgag ctcttgaagt ttgctctttg tatcaatatt accgttattt acagcgaatt
     1021 cc
//
LOCUS       DAPHDW010000342         1020 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.350, whole genome shotgun sequence.
ACCESSION   DAPHDW010000342 DAPHDW010000000
VERSION     DAPHDW010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1020)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1020
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.350"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>1020
                     /locus_tag="RH057_004620"
     CDS             <1..>1020
                     /locus_tag="RH057_004620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130831.1"
                     /translation="EVNDQDGNGIPDFIDSQLAFFNKASSPNVSGDLYPNAPRFSWST
                     ESNKISTVNKNWEGILNANPGAYVTRETISTNVEQYNYNGINGSDIISIKNSLFGLDR
                     TQVDRQPVGLFTGEGNDIIVTGQDYGRNTSLGYTDHANITNMGGGDDTLVVGAGNNNV
                     TLYVNDDGQLRATTDGYDGLYSINYNQINPGVTGGTISGTDISMGEGNDTILALGTEG
                     NTADAIINTNIDLGQGNDFIYINGNVSSAWNTSVNIRGNEGVDTLFIDNGDISSLQFS
                     GFEVIKLGRNGGVNIREVDLLKSGADAIEGGDLKIIREASSRVKLEGDFIQKGIETVE
                     GVNYL"
BASE COUNT          329 a          148 c          240 g          303 t
ORIGIN      
        1 cggaagtgaa cgatcaggac ggtaacggca tccctgattt tattgattct cagttggcct
       61 tctttaataa ggcatcgtct ccgaatgttt cgggggactt ataccctaat gcaccgagat
      121 tttcttggag tactgagtca aataaaatat ctacagttaa caaaaactgg gaaggaatat
      181 taaatgctaa tccaggagct tatgttacac gagagaccat cagcacaaat gtagaacaat
      241 ataattacaa tgggattaat ggttctgata ttatttcaat taaaaactcg ttgtttggat
      301 tagacagaac acaagtcgac agacaacctg ttggtttatt cacaggagaa gggaatgata
      361 taattgttac tggtcaagat tatgggcgta atacatcttt aggatataca gaccatgcta
      421 atataactaa catggggggg ggcgatgata ctcttgttgt tggtgctgga aataataatg
      481 taacattgta cgttaatgac gatggtcaac ttcgtgctac tactgatggt tatgatggcc
      541 tgtattctat taactataac cagattaatc ctggcgtaac tggtggaaca atttcaggga
      601 ctgatatctc aatgggggaa gggaatgata ccattctggc tctgggtact gaagggaata
      661 cagctgatgc cattattaat acaaatatag atttgggtca gggaaacgac tttatctata
      721 tcaatggtaa tgtttcatca gcatggaata cttcggtaaa tatcagaggt aatgaaggtg
      781 ttgatacact ttttattgat aacggtgata tttcctcttt acaattctct ggtttcgaag
      841 taattaaact tggaaggaac ggaggggtga atataagaga agtcgattta ctgaagagtg
      901 gagcagatgc tatagaggga ggtgacctta aaataataag agaagcttcc tcgagagtta
      961 aacttgaagg tgattttatt cagaaaggaa ttgaaacggt ggagggtgta aactatttag
//
LOCUS       DAPHDW010000343         1008 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.351, whole genome shotgun sequence.
ACCESSION   DAPHDW010000343 DAPHDW010000000
VERSION     DAPHDW010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1008)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1008
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.351"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            66..959
                     /locus_tag="RH057_004621"
     CDS             66..959
                     /locus_tag="RH057_004621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130832.1"
                     /translation="MPTISSSKVPSPKSWDEFEEITLSAAKLRWNSTDFYRHGRQGQK
                     QDGVDIWGYDGKRHIGVQCKNTTGELGLAVVETEIANAEKFMPKLDHLYIVTTAPRDA
                     KLQKSVREISQKHSKAGDFRIDVLFWDDICNDLAKDEDVFFGHYPQFKSDDNPGKKHD
                     IALWSELTTLLRSDGVIGFLDRTNMAGFSFRESQLEPIREFYFEWNNPEHEFITPELE
                     KINKELWSEVDRYYNLIANETFPANNPQFSTVPPEWEFEQPDRFWRVVNALHDHAGKI
                     VKLHADIVRTGREKFLNSGNK"
BASE COUNT          324 a          189 c          231 g          264 t
ORIGIN      
        1 gttagcaaca taatagtcaa catatgatat aaacataaaa ccaaacaaac tttcatgaga
       61 aaataatgcc tacaatctcg tcgtctaaag taccatcccc aaagtcttgg gatgaatttg
      121 aagaaattac cttatccgca gccaaattgc gttggaactc tactgatttt tatcgtcatg
      181 gacggcaggg acagaaacag gacggtgttg atatctgggg atatgatggt aaacgtcaca
      241 tcggagttca atgcaagaat acgacaggcg agctgggttt ggctgttgtt gaaactgaga
      301 tagcgaatgc tgaaaaattc atgccaaaac tggatcatct ctacattgtc acgacggccc
      361 caagagatgc gaaacttcaa aaatcagtaa gggaaatctc ccagaaacat agcaaagctg
      421 gagatttcag gatcgatgtg cttttctggg atgacatttg caatgatttg gcaaaggatg
      481 aagatgtatt cttcgggcac tatcctcaat tcaagtctga tgataatccg gggaaaaagc
      541 atgacattgc attatggagt gaactcacga cgcttcttcg ctctgatggt gtgattggct
      601 tcttggatcg tacaaacatg gccggatttt cgttccgtga atcacagctt gaaccgattc
      661 gtgaatttta tttcgagtgg aacaacccgg agcacgagtt cattacgcct gagttagaaa
      721 aaattaataa ggaactttgg tcagaggttg ataggtacta caacctcatt gccaatgaaa
      781 cctttccagc aaataatcca caattcagta cagtaccccc tgaatgggag tttgaacaac
      841 cagataggtt ttggcgggtt gtgaatgcgc tacatgatca cgctgggaag atcgtaaaac
      901 tgcacgcaga tattgtacgt accgggcgag agaaattttt gaatagtggt aataaataat
      961 tatttgataa tcagaaaagt aaatgtaagg ggaccggata tgactacc
//
LOCUS       DAPHDW010000344         1001 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.352, whole genome shotgun sequence.
ACCESSION   DAPHDW010000344 DAPHDW010000000
VERSION     DAPHDW010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.352"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(218..889)
                     /gene="queE"
                     /locus_tag="RH057_004622"
     CDS             complement(218..889)
                     /gene="queE"
                     /locus_tag="RH057_004622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HDV3130833.1"
                     /translation="MQYPINEMFQTLQGEGYFTGVPAIFIRLQGCPVGCAWCDTKHTW
                     EKLEDREVSLFSILAKTKESDKWGAASSEDLLAVIGRQGYTARHVVITGGEPCIHDLL
                     PLTDLLEKNGFSCQIETSGTHEVRCTPNTWVTVSPKLNMRGGYDVLSQALERANEIKH
                     PVGRVRDIEALDELLATLTDDKPRVIALQPISQKDDATRLCIETCIARNWRLSMQTHK
                     YLNIA"
BASE COUNT          278 a          245 c          211 g          267 t
ORIGIN      
        1 gtaaatattt aaaataactc tatacaaact aaaatctacc aaactttacc gcaataattt
       61 tcactccagc gaaaaattaa tgccacagaa tttgtagaga atgctaatga ttagccctgg
      121 atgggtaaaa tggaggagtt ttcagaggcg acaaacaata taaatgagta gaagtcttta
      181 caggttccac ccatttataa cgcttataaa tgtttaatca ggcaatattt agatatttat
      241 gtgtttgcat cgacaaacgc caattacgcg caatgcaggt ttcaatgcac aaacgtgtgg
      301 catcatcctt ttggctaatc ggctgcagtg caatgactcg cggtttatca tcggtcagcg
      361 ttgccagtag ttcatccagt gcttcaatat cacgtacgcg ccccactgga tgcttgattt
      421 cgttggctcg ctccagtgcc tgtgacaaca cgtcatagcc gccgcgcatg ttcagctttg
      481 gcgatacggt aacccaggta ttcggtgtgc agcgtacttc atgagttcca ctggtttcga
      541 tctggcagct aaaaccgttc ttttcgagca ggtcagtcag tggcagcaaa tcatgaatgc
      601 aaggctcacc acccgtaatc accacatgcc gcgcggtata tccctgccga ccaatgacag
      661 ccagcaaatc ttcactgctc gccgcccccc acttatcact ctctttggtc ttcgccagaa
      721 tgctgaaaag ggagacttcc cgatcctcaa gcttttccca ggtgtgtttg gtgtcgcacc
      781 aggcacagcc aaccgggcat ccctgtaaac gaataaaaat ggcggggacg ccggtaaagt
      841 aaccctcacc ttgcagggtc tggaacatct cgttaatcgg gtactgcata gcattctctg
      901 tgaagtggat aattgttaat tattgcagat cctgccacaa caatcatgtc ttattaacat
      961 tctgttacag gcagatttaa agaaaaatgc gaaacatatc g
//
LOCUS       DAPHDW010000345         1000 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.353, whole genome shotgun sequence.
ACCESSION   DAPHDW010000345 DAPHDW010000000
VERSION     DAPHDW010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.353"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(259..909)
                     /gene="zinT"
                     /locus_tag="RH057_004623"
     CDS             complement(259..909)
                     /gene="zinT"
                     /locus_tag="RH057_004623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HDV3130834.1"
                     /translation="MAIRLYKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDANVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYHKGYA
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
BASE COUNT          291 a          184 c          201 g          324 t
ORIGIN      
        1 tatttaaaaa attgatagtg catattgtaa ccagccaata taagcaatgg cgtgagcaca
       61 ggtatctttc tctgttggcc gtattgtgta tggaatccgt tattggttaa ctcaatgcgg
      121 gttagcttat taatatacag acacgtcata gtgataagcg tgtctgtagc tagcttgcgt
      181 tcagtggcgt ttgtggatgg accagtggca gttatgataa cttactaaag cggcatcgag
      241 gcgttatcat gagaatactc aatgagacat catttcctcg accacttctt cgctactcaa
      301 ctgatatgga taatacgttg gccagttttc catttcattt aataaagatt gctgtgaatc
      361 attacccata aaaatgtgaa aatgagatga ttttcgtggt gcaattatat gatcgctaaa
      421 ttgtatatat ttaggggctt tgctttcagg atctttacat tcgaataagt aacgaacgcc
      481 tttcttgcct gatttatagg tgagtatttt gtatccatcg taatcatatt tacacgatgt
      541 tgtttcatta tttctatgga attcaacaat gccgtcctca atgccaatca tctcgatatc
      601 tgttgcataa cctttgtgat aataatcttt aatttcagca aatgttttag ttttatctgc
      661 atccgctttc ttctgaaaga cggggtcaag tttgccactt tgcagtaaag gataaacgga
      721 ttgccagact ccatcccagt cactgagcgt tcggttttgt acattggcat catcaaaaac
      781 accattagca gctttttgtt cgacctctgt taagggtttg ccgtgtgagt gatgaccatg
      841 cgaaaaggca ggagcgctaa caataaagac acctaaagca acagccagtt tgtaaagacg
      901 aatcgccaaa acagtttcct ccagagttaa tgtatatgat gtgtaatgtt atattgtaac
      961 atatagcaaa atgcaacaac tcttaggaaa cgagagcatg
//
LOCUS       DAPHDW010000346          963 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.354, whole genome shotgun sequence.
ACCESSION   DAPHDW010000346 DAPHDW010000000
VERSION     DAPHDW010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..963
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.354"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(27..320)
                     /gene="cas2"
                     /locus_tag="RH057_004624"
     CDS             complement(27..320)
                     /gene="cas2"
                     /locus_tag="RH057_004624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated endoribonuclease
                     Cas2"
                     /protein_id="HDV3130835.1"
                     /translation="MSMIVVVTENVPPRLRGRLAIWLLEVRAGVYVGDTSKRIREMIW
                     QQISQLAGCGNVVMAWATNTESGFEFQTWGENRRIPVDLDGLRLVSFLPVDNQ"
     gene            complement(317..832)
                     /gene="cas1"
                     /locus_tag="RH057_004625"
                     /pseudo
     CDS             complement(317..832)
                     /gene="cas1"
                     /locus_tag="RH057_004625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311636.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas1"
BASE COUNT          266 a          258 c          190 g          249 t
ORIGIN      
        1 ttccttattt ttaaagaacg gataacttat tgattatcaa caggaagaaa agaaaccaaa
       61 cgcaacccat ccaaatccac cggaatacgt ctattttcac cccaggtctg aaattcaaaa
      121 cctgactcgg tattggtcgc ccaagccatc actacatttc cgcaacctgc cagttgagag
      181 atttgctgcc agatcatctc ccgaatacgt ttggatgtat ctccgacata cacaccggca
      241 cgcacttcca gtagccagat agcgagccgt ccacgtaaac gtggaggaac attttccgtt
      301 acaactacga tcatactcat ccgccgcgtc ctcgatgacc gctatcgcct aacgtttcag
      361 gttcagggat ggcaggcggt aacatatccg gcgcgggttg tggtggttca atttcaccag
      421 cggcaagcac ttcctcaatt aacggaatta acttgcccgt taatttacta ctacggaaaa
      481 tatcgcgaca ggccaacctg acttctttat ctggttctgc gggttgcctc gctgctattt
      541 caaatgcctt tggcacaacc aaatcaaatt taatgatatc ggctatgtca taaacaaatg
      601 aaagcggttt gccgctatgg ataaaaccaa tagcgggcgc atatcccgca gctaatactg
      661 cagcttcaga aataccgtac aggcatgatg tggcagcact gatgcaacga ttgacaacat
      721 cgcctttttc ccagtcttta ggatcgtatt tgcgaccatt ccatttcaca ccatattgtt
      781 tcgccagtaa tgcatacgtc tggcgaacgc gggatccctc aattccccgt acatccagcg
      841 aatgccagca aagaaggtga tagtcgtcgc ccccgtcttt ctcgccgcgt cgggtttttc
      901 cccagaaatc aaaaaaagtc acaatatttt tatccttcag taaacttaaa ggatatttac
      961 gca
//
LOCUS       DAPHDW010000347          955 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.355, whole genome shotgun sequence.
ACCESSION   DAPHDW010000347 DAPHDW010000000
VERSION     DAPHDW010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 955)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 955)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..955
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.355"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(81..>955)
                     /locus_tag="RH057_004626"
     CDS             complement(81..>955)
                     /locus_tag="RH057_004626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HDV3130836.1"
                     /translation="LLLMAGKVFAFSCNVDGGSSIGAGTTSVYVNLDPVIQPGQNLVV
                     DLSQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVTYPFPLTTNTNVL
                     DIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGSGNPRNFTWNIIS
                     NNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFYLSGATTDSARQV
                     FANTAPDATKASGVGVSLMRNGKILATGENVSLGTVNKSKVPLGLSATYGQTGNKVSA
                     GTVQSVIGVTFIYE"
BASE COUNT          267 a          219 c          203 g          266 t
ORIGIN      
        1 tgtaggataa tagcctttac aaaataatta tccgcgcaat tttcgtgaat ggagcgcata
       61 gactaagatc aaaatcacaa ttattcatag ataaaagtga caccaatgac tgactggacg
      121 gttccggctg aaaccttgtt tcctgtttgt ccataggttg cgcttaaacc cagaggtact
      181 ttgcttttat ttactgttcc cagggagaca ttttctcccg tagccagtat ttttccatta
      241 cgcatcagag agacacccac tcccgatgct tttgttgcat caggtgctgt gttagcaaac
      301 acttgtcggg cactgtcagt ggtggcaccc gaaagataaa atgagagctt ttgttcgctt
      361 gagcaatata cgccaagggg tatttctgcg cttccaggaa agtccgggag atccacggtg
      421 acattacgac tatcaacagt gcagccgcct gttggcataa caacactgtt atttgaaata
      481 atattccacg taaaattacg cggatttccg cttcctaatg ttgcaatttt atacatatga
      541 atacgggcaa taacttcccc ggcttttatg acaactccac cggccgcccc gactggggta
      601 atatagagtt ttaacggtaa tggcatcggt gtcttatcac caatatccaa tacgttggta
      661 tttgtcgtca acgggaaggg gtaggtcaca ttattccagt acagcgatcc tttatatgac
      721 tgtagagagc ctgcgaaagc tgagccttgt accaggttta tatgatcagt gtcgtaccag
      781 ccgccgtaat cattccagca acttatatgc tgagacaagt ctacaaccag attctggccc
      841 ggctgtatta cagggtcgag attaacataa acagaggttg taccagctcc aatacttgac
      901 ccgccatcga cattacaact gaaggcaaaa actttacctg ccattaatag aagat
//
LOCUS       DAPHDW010000348          942 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.356, whole genome shotgun sequence.
ACCESSION   DAPHDW010000348 DAPHDW010000000
VERSION     DAPHDW010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..942
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.356"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            125..>942
                     /locus_tag="RH057_004627"
     CDS             125..>942
                     /locus_tag="RH057_004627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130837.1"
                     /translation="MKNWAPEHTKEIKLWLDISTYRKFEDLSLIQFYHELWARNLFFK
                     EYREDFESKELMVYFSKIFSGNPFLIEEGQLGYMAPANKLFQPPHFFLTTLDRLAETS
                     IIAMQRGGFVWHEGDNYSINRELREESLSDIMPDQFSRTVMFEIDLASGTDEEIAESL
                     KTALPQWRKVKGIDENPLESVRFGYGTIKKLISYRVIPMLDILVWAAVKKIRVSDDRL
                     SRLLYTDNDEESEVRLPNQIKDTDRPLALKACTIAFIRQFNHFINKNNHLKQIKV"
BASE COUNT          311 a          161 c          197 g          273 t
ORIGIN      
        1 ttataccaac aagtagctaa agacatttca actgtgtgtg gaaagtcgca taacttgcat
       61 aagttgagta agtctgacaa ttttaacact aacagattat ggatttttgt tggagtgtat
      121 acttatgaaa aattgggctc cagagcatac gaaagagatc aagttatggc tggatattag
      181 tacttaccgc aaatttgaag acctctcatt gatacagttc taccatgagt tatgggctcg
      241 gaacttgttc tttaaggaat atcgagaaga tttcgagagc aaagaactta tggtgtattt
      301 ctcgaaaata ttcagcggta atcccttttt gatcgaagaa gggcaattgg gatacatggc
      361 tcctgccaac aagctctttc aaccgccaca tttttttcta accacacttg accgactcgc
      421 tgaaacgagc attattgcaa tgcagcgtgg aggatttgtt tggcatgaag gtgataatta
      481 ttctataaac agagagcttc gggaagaatc attatcagat attatgccag atcagttttc
      541 aagaacagtt atgttcgaaa ttgatcttgc gagtggtaca gacgaagaaa tcgctgagtc
      601 gcttaaaaca gctctccctc aatggcgtaa agtgaagggt atcgatgaga acccattaga
      661 gtcagttcgc tttggatatg gaacgatcaa gaagttaatc agctatcgtg taattcctat
      721 gctggacata ttggtatggg ctgccgttaa aaaaattaga gtttctgatg acagattatc
      781 cagattgtta tatacagata atgacgaaga aagcgaagta agattgccta atcaaataaa
      841 agataccgac agacctttag cacttaaagc gtgtacgatt gcctttatca ggcagtttaa
      901 tcacttcata aataagaata accatctaaa acaaattaaa gt
//
LOCUS       DAPHDW010000349          941 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.357, whole genome shotgun sequence.
ACCESSION   DAPHDW010000349 DAPHDW010000000
VERSION     DAPHDW010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 941)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..941
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.357"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(361..941)
                     /locus_tag="RH057_004628"
                     /pseudo
     CDS             complement(361..941)
                     /locus_tag="RH057_004628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308587.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
BASE COUNT          209 a          296 c          195 g          241 t
ORIGIN      
        1 aacagattct gtcgatgatt ctggattatc aacatccact acataagtat acgcaaaatg
       61 ccccccaact ccgccgccaa ccgttacacc tactgaattg ctgactcccg atgtaggtaa
      121 agacttggtc tcagaatatg tgatagccgc atttatccca ccaccaattc ctgcatgtcc
      181 gcatgctgta gcataaatac aaatatcaga taacatttca cctttctttt tagcaaacgc
      241 aacgccaagt cctgcatgac cacctaatga tattagatct ccactgccag taatatcaat
      301 tgatgatgtt ccatatgaat catcatgcca aacatcatat aataattgat ataaattata
      361 caaacccaaa ggatccatcc ttattatcgg attcagtggg taattataca ggttccatcc
      421 ccccgccagc ccaatggggt cttgtgtaat atacctcccc tgtagcgggt catagtaccg
      481 gttccggtta tagtacagcc ctgattcctc atcatgctgc tgccccggca gacggtatgg
      541 ctggtgcagg taatacgggt tctcttcatt aagctggttg ccccattcat catactcccc
      601 gcgccacgcc gtattgccgt cttcgctgat gagcgccagc ggcaggcccc ggtggtcgca
      661 gtggtaaaaa tgaacttttc gcgccggtgt gtattccggc tccacctgtc tggccagttg
      721 ctccaccgtc agcccgcact gcgcaagcca cgcccggctt tcactgctca cgcggtctgc
      781 ccggatttct tcctccagcc tgtccagcag ccgcaccagt tcagccggaa ataccacgcc
      841 gtgcccgtcc tcgctccctt cctgctgcag cttctccgcc aggctgcggc gctgcgcttt
      901 ctcccgctcg ccgttctctg tctcgacccg gatgagcggc g
//
LOCUS       DAPHDW010000350          935 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.358, whole genome shotgun sequence.
ACCESSION   DAPHDW010000350 DAPHDW010000000
VERSION     DAPHDW010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 935)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..935
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.358"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..434
                     /locus_tag="RH057_004629"
     CDS             <1..434
                     /locus_tag="RH057_004629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TIGR04141 family sporadically distributed
                     protein"
                     /protein_id="HDV3130838.1"
                     /translation="FIHHGGGKSKIEFCDLIRGQSDFIHVKYYTGSQSMSHLFSQGFI
                     GSELFISDSEFRGKLNEKLPAHIKLADHINRPDAKKYKLVFAIASNKNLPEDLPLFSK
                     VNLKNFHKSITNFGYEVRICKIAVDPDIYKKKLCKPQKNKL"
     gene            complement(594..806)
                     /locus_tag="RH057_004630"
                     /pseudo
     CDS             complement(594..806)
                     /locus_tag="RH057_004630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003845876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
BASE COUNT          298 a          171 c          167 g          299 t
ORIGIN      
        1 aattcattca tcatggtggt ggtaaaagta agattgaatt ttgtgatctt atcagaggtc
       61 aatcagattt tatccatgta aaatattata ctggttcgca aagtatgagc catctttttt
      121 cacaaggatt tataggttct gaacttttta taagtgactc tgaatttcga ggcaaactta
      181 atgagaaact acctgctcat ataaaattag ccgatcacat aaatagacca gatgctaaaa
      241 aatataaact agttttcgcc atagcttcta ataaaaatct tcccgaagat ttacctttgt
      301 tttctaaagt aaacttgaaa aatttccaca agagtattac caattttggg tatgaggtca
      361 ggatctgcaa aattgctgtg gacccagata tttataaaaa gaagctatgt aaaccacaaa
      421 agaacaagct ttaaataaat ttttcaatgc cagagacgtt tgccagttca tgcagaaaat
      481 cgcttttgta agttacagtc catgtatctt ggtttagaac aaagtggtgt caaaaaaggt
      541 ttatgccttg ggagggaata aggggctggg aaatttaacc ccttatagag ttgttatgcc
      601 gcaatagctt taaatttttg agcaatgaca taatcactcc accactgcat gagaaccatt
      661 cgttctgcga gatactcggc acgattatag gctgcaatta tttaatcttt cttcgagtgg
      721 gcaagggccg cttctaagac accagtcctg aacttaccag attcctctgt cgctgttctc
      781 gcaatagatc tcattctgtt aaccataagt tcacctcctt aaaccgttcg aataattacg
      841 gcttatactg cctacttatg ctaatatcta agagaagcca ttgtggttgt gtaagtatac
      901 ttcttggttt atttcgacta tagaaaacca tacct
//
LOCUS       DAPHDW010000351          931 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.359, whole genome shotgun sequence.
ACCESSION   DAPHDW010000351 DAPHDW010000000
VERSION     DAPHDW010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.359"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(567..742)
                     /locus_tag="RH057_004631"
                     /pseudo
     CDS             complement(567..742)
                     /locus_tag="RH057_004631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612254.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          290 a          187 c          193 g          261 t
ORIGIN      
        1 gtataagtga cttcaaaata catagctaca ttattaaaac aagtgaatat ccggaggagt
       61 ttttttccaa tgcagtatgg ggtaaaaaaa atataaaacc gggggaatat tactctgtgc
      121 aggctgtttc tatgtttacg acaaaccagt ttattctcct ttgtcccaag gatattataa
      181 ggttttatcg ggaaagctgg aagttcgacc tgcttaatgt agacttcgat acaaatacca
      241 ggaagaaact ccaggaagaa gcagcatatt gcgtaccaat ttctgcaata tctgtaaaac
      301 gatgggatat gcctcttcag ggctcgaaac cctcaaatta gaaggccgct cgtgcgactg
      361 tattagatag taatgcggta tggaaaaata gtgatggcaa gatgaacttg acttggctaa
      421 tcaaataccg gcgtagtgat taggaaacag ggctcggata acattattgc gcggttcatt
      481 gaacctactg atgacgccgg atatatagtc gtaaaccgtt tcgaaccagt acttatatca
      541 ccaggagagt ccatatatga ccaccgttta cagtaaaaac ggtatctgtt ttctgatccc
      601 tccagcgtca aactaccgtt gtaatggtga ggtctggtgc tattgtaata attcagaata
      661 taatctctga tttatttccg aacctcgccc ttgcctgcgt aaccatccgt cgcccccatc
      721 ctgttttcag actgcggaaa aaggcaagtt tttatccccc cacagagatt caatcatata
      781 tcgcgtgttt tggaagtgac agttcttatt cctgagaata cagcatgtgc aacacactcg
      841 gcatccatcg ataccacttt tatctctaat aggcaggata aaaaacaaga ctgtgaatta
      901 aagtaatcgg caaatgaatg agttagaaga t
//
LOCUS       DAPHDW010000352          931 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.360, whole genome shotgun sequence.
ACCESSION   DAPHDW010000352 DAPHDW010000000
VERSION     DAPHDW010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.360"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(107..439)
                     /locus_tag="RH057_004632"
     CDS             complement(107..439)
                     /locus_tag="RH057_004632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HDV3130839.1"
                     /translation="MAGWFELDKSSNEQYKFVLKAGNGEIILTSELYTTKSSAENGII
                     SVQANSPLDERYDLKDSSNGKSYFNLRAANHHVIGTSQMYSTKQSREKGIASVKTNGT
                     TTTIKDNT"
BASE COUNT          254 a          170 c          191 g          316 t
ORIGIN      
        1 taatgattcg gaatcatttc taccgttggc tggggtacat aaaagcgtaa tatttttatt
       61 cattctgata aagccaagtc acatggcttg gcttcagata tattgattaa gtattatctt
      121 taatcgttgt tgttgttccg ttggttttca cggaagcgat acctttttca cgggattgtt
      181 tggtcgagta catctggctt gtaccgatca catgatgatt agccgcccgt aggttaaaat
      241 aggacttgcc gttagatgag tctttcagat cgtagcgttc atcaagtggg ctgtttgcct
      301 gaactgatat aatgccattt tctgcagaac tcttggttgt gtaaagctcg ctggtgagga
      361 taatttctcc attaccggct ttcagaacaa atttatactg ttcattactg ctcttatcca
      421 attcgaacca accagccata ttaactcctt gataaagttg tgatttttac ggcccagtga
      481 tgaagaacga agcgggcaag ttcagtatgc ctgaaacaaa aattgtttcc aatatgttat
      541 gtttgtatcg gtagctggga acaagactac acactacgaa atgaggctta gctgtcgccc
      601 accagtgttg gcaggtatgc cttagtggga gtcttagagc ggagggtatc ttgaatcaat
      661 gtctattgcc atatttacac gacacacgat atattttctt tagagtcata tcttaggaaa
      721 caataacggc gacttgagca aaagtatcta atatcaatag attttcgtta atttaaaaaa
      781 ataggtgctc tctcataaaa tctgtagggt acttggtcgt gaataaattg ctgagttcat
      841 gttgcgatgt cattatttgt catcttccat gtctcataat tatcctaacg tataggttca
      901 atgaaattcc tttattcctg tgtctaatct a
//
LOCUS       DAPHDW010000353          928 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.361, whole genome shotgun sequence.
ACCESSION   DAPHDW010000353 DAPHDW010000000
VERSION     DAPHDW010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 928)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..928
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.361"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..492)
                     /gene="sctT"
                     /locus_tag="RH057_004633"
     CDS             complement(<1..492)
                     /gene="sctT"
                     /locus_tag="RH057_004633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     subunit SctT"
                     /protein_id="HDV3130840.1"
                     /translation="MGEAILYQLHSLLAATALGFCRLAPTFYLLPFFASGNIPTVVRH
                     PIIIVVSCALVQHYHYELLNLNEIDIALFAAREIIIGLFIACLLASPFWIFLAIGSFI
                     DNQRGATLSSTLDPATGVDTSELARLFNLFSAAVYLTKGGMNFILETLWQSYNLWPSG
                     NFNF"
     gene            complement(494..753)
                     /locus_tag="RH057_004634"
                     /pseudo
     CDS             complement(494..753)
                     /locus_tag="RH057_004634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscS/YscS/HrcS family type III secretion system
                     export apparatus protein"
     gene            complement(763..>928)
                     /locus_tag="RH057_004635"
     CDS             complement(763..>928)
                     /locus_tag="RH057_004635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EscR/YscR/HrcR family type III secretion system
                     export apparatus protein"
                     /protein_id="HDV3130841.1"
                     /translation="DLVISSVLLTLGMMMMSPVTISTPIKLILFVAMDGWTMLSKGLI
                     LQYFDLSINP"
BASE COUNT          324 a          208 c          156 g          240 t
ORIGIN      
        1 aaaattaaaa ttgcctgaag gccataaatt ataactttgc caaagagttt ccagaataaa
       61 gttcattcca cctttagtaa gataaaccgc agcagagaaa aggttaaata atcttgctaa
      121 ttccgaggta tcgacacctg tagccggatc caaagtacta cttaatgttg caccgcgttg
      181 gttatcgata aagctaccta tagcaaggaa aatccaaaac ggggatgcta ataagcatgc
      241 aataaacagc cctatgataa tttctctggc agcgaacaaa gcaatatcga tttcatttaa
      301 gttcaataat tcatagtggt aatgctgaac taatgcacat gataccacta ttattattgg
      361 gtgacgaact actgttggta tattcccact ggcgaaaaat ggtaacaaat agaaagttgg
      421 tgccaatcgg caaaaaccga gcgctgtagc agccagcaga ctatgcaact gatagagtat
      481 cgcctccccc atgctatcct ctcgcgaatg ctaaattcag catttcgata ccaaatgaat
      541 ataatttttc accataccac ccgctcatta aaaaaagcaa atacttacgc acaataattt
      601 cacgccaaat ggcaacgtct gttcttgcaa ctgcgttacc gtttgaaaaa gccctaccaa
      661 taaaccaaca aatgtcgcaa ctgcaatagg tcctgcagac ataactaaaa tcagatacag
      721 cgccctattc cccgcaaaaa caatatcgtc catatttttt cctcagggat taatggatag
      781 gtcaaaatat tgtaaaatga gcccttttga taacattgtc caaccgtcca tcgcgacaaa
      841 caaaattaat tttatcggtg tagatatggt taccggactc atcatcatca tacctaacgt
      901 taacagaacg ctggatataa ccagatcc
//
LOCUS       DAPHDW010000354          925 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.362, whole genome shotgun sequence.
ACCESSION   DAPHDW010000354 DAPHDW010000000
VERSION     DAPHDW010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.362"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(220..717)
                     /locus_tag="RH057_004636"
                     /pseudo
     CDS             complement(220..717)
                     /locus_tag="RH057_004636"
                     /inference="COORDINATES: protein motif:HMM:NF024997.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          244 a          209 c          201 g          271 t
ORIGIN      
        1 ttatattgac atcttattat ctactcagac gtgatgagta tatcattggc gttatttcca
       61 tagccggaga tcggtgagtt acggcgtttt tcaatatggt tgtcgcctga tgtaacaaaa
      121 ttacgttatg ccgcttcttt ttctggattc aggtagactt ctgatatata gcccagttac
      181 gtagatgctt gctccaacgt tcaggatgtt gctcgcgtgc tagctgttat accacttttc
      241 tgactcggct ggtgtgacat attttatccc accgtgctta tgttcaaagt tgtaccagtt
      301 tacaaaaccg tttacccata ctctgacttc atcaatagta caaaaaccgt tctcaggcca
      361 ccagggacaa tatttgagag tccggaacaa agattctgag taagggttat cattgctgac
      421 tcttggccgg aaataagaac ttagcacacc aagcgcatac attttcgcca acaacgtgca
      481 actgcgcatg ggaccaccgt tatcagaatg gagtaccaga ttgttggatg aacatttttc
      541 attccatact gcacgctgga gtagttctgc tgcatgtttt ccagactcgc tggcaaatac
      601 ctctgcagca ataattttgc gactgtaaat atcgacgatc atatagaggt aaaaatgctg
      661 gcctctgacc caggacggca aatagctgat gtcccaactc cagacttgat tcggcgcaac
      721 ggccctgagt acatcaggcc gcttatactg gcattaacgg gatcttttcg tcaactggtt
      781 ggctgatttc agtacacgat aaaagtttga ttcagaagca atataaattc ctctgtctgc
      841 cagagttggt acgatcacat ttggaggtaa ggaagcgaac tctggcgatt acaatgtagc
      901 atagcgccag gttatcgctc gccac
//
LOCUS       DAPHDW010000355          921 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.363, whole genome shotgun sequence.
ACCESSION   DAPHDW010000355 DAPHDW010000000
VERSION     DAPHDW010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.363"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            67..852
                     /locus_tag="RH057_004637"
     CDS             67..852
                     /locus_tag="RH057_004637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HDV3130842.1"
                     /translation="MNSKKLCCICVLFSLLAGCASESSIDEKKKKAQVTQSNINKNTP
                     QQLTDKDLFGNETTLAVSEEDIQAALDGDEFRVPLNSPVILVQSGNRAPETIMQEEMR
                     KYYTVSTFSGIPDRQKPLTCNKNKDKNENEDVASAENMNWMQALRFVAAKGHQKAIIV
                     YQDTLQTGKYDSALKSTVWSDYKNEKLTDAISLRYLVRFTLVDVATGEWATWSPVNYE
                     YKVLPPLPDKNEASTTDMTEQQIMQLKQKTYKAMVKDLVNRYQ"
BASE COUNT          306 a          185 c          195 g          235 t
ORIGIN      
        1 agtatataat taaaaatcgt tagctaaaca ttaccatgat tatgacaggc gagggagtgt
       61 ccaattatga attcaaaaaa gctttgttgc atatgtgtgt tattctcgct gcttgcagga
      121 tgtgcctctg aatcttctat tgatgaaaag aagaaaaaag ctcaagtcac acaaagtaat
      181 attaataaaa acactcccca gcaactgaca gacaaagatt tattcggtaa tgaaaccact
      241 ctggccgtat ccgaagagga tattcaagct gcgcttgatg gagatgagtt ccgcgttccc
      301 cttaattctc ctgtaatact tgttcaatcc ggcaaccgcg caccggaaac cattatgcag
      361 gaggagatgc gaaaatatta tactgtttcc acattctctg gtatcccgga caggcaaaag
      421 cctctgactt gtaacaaaaa caaagataaa aatgagaacg aggatgttgc cagtgctgag
      481 aatatgaact ggatgcaggc actgcgtttt gtggctgcaa aaggacatca gaaagcgatt
      541 attgtttacc aggatacatt gcagacagga aaatatgact ctgcgctgaa atcaacagta
      601 tggtccgact ataaaaatga aaaactcacc gacgctatct ccctgcgcta cctggtacgt
      661 ttcacgctgg tggatgtggc aacaggtgag tgggctacct ggtcgccggt gaattatgaa
      721 tataaagtgc ttccaccatt gcccgacaag aatgaagcca gtacgactga tatgacagag
      781 cagcaaatca tgcaacttaa gcaaaagacc tataaagcga tggtaaaaga tttggttaac
      841 cgctatcaat aataaattat atctgccgcc aggaatttct ggcggcaata atacaaaatt
      901 ctttgcataa gaaatttttc t
//
LOCUS       DAPHDW010000356          911 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.364, whole genome shotgun sequence.
ACCESSION   DAPHDW010000356 DAPHDW010000000
VERSION     DAPHDW010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.364"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..757
                     /gene="ydeO"
                     /locus_tag="RH057_004638"
     CDS             <1..757
                     /gene="ydeO"
                     /locus_tag="RH057_004638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HDV3130843.1"
                     /translation="LVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPYER
                     HFKEIGFTENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERNHH
                     NFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCLYI
                     SESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYAFR
                     KHFGNSPKRVSKEYRCQSHTGMNTGNTMSALAI"
BASE COUNT          273 a          182 c          179 g          277 t
ORIGIN      
        1 gctcgtttgt tctgttatat ttattcatca tgccttcaac gctaacattt tagataaaga
       61 ttacgccttc tctgacggcg agatcctgat ggtagataac gctgttcgta cgcattttga
      121 accttatgag cggcatttta aagagatcgg atttactgaa aataccatta aaaaatatct
      181 acaatgcact aacatccaga cagtgacggt gcctgttcct gcgaagtttt tacgtgcttc
      241 aaatgtaccg actggattgc ttaatgaaat gattgcttat ctcaactcgg aagaacgcaa
      301 tcatcataat ttttcagaac ttttgctttt ttcttgcctg tctatttttg ccgcatgcaa
      361 aggtttcatt acactattaa ctaacggtgt gctatccgtt tccggaaaag tgagaaatat
      421 tgtcaacatg aagctggcgc acccatggaa gctgaaagat atttgtgact gcctgtacat
      481 cagtgaaagc ctgttgaaga aaaaactaaa gcaagagcaa acgacattct cacagattct
      541 attagatgcc agaatgcagc acgcaaaaaa tttgatacgc gtagaaggtt cagtcaataa
      601 aattgccgaa caatgtggtt atgccagtac atcttatttt atttatgcgt tccgcaaaca
      661 tttcggcaac agtccgaaga gagtttctaa ggagtaccgt tgtcaaagtc acacgggtat
      721 gaatacgggc aacacgatga gtgctttagc tatttgatta tttgctaacg agtagtcaac
      781 cacgcacgct acgtaagaat tatatggggc agccattccc tgccccgcgt tgtttttagg
      841 cgatatattt attgaaataa ataagtgaca tccatcacat atttatgcac ttgcataacc
      901 tgttgcatga t
//
LOCUS       DAPHDW010000357          873 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.365, whole genome shotgun sequence.
ACCESSION   DAPHDW010000357 DAPHDW010000000
VERSION     DAPHDW010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..873
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.365"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..655
                     /locus_tag="RH057_004639"
     CDS             <1..655
                     /locus_tag="RH057_004639"
                     /inference="COORDINATES: protein motif:HMM:NF016159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HDV3130844.1"
                     /translation="FDEIESKIKKGLPLNRQRLQKSCLVSFSNTYDVEEILISDSGAS
                     ISEYWWKNFLSTVELQSSELNTKNAFGSIENFLKREVEKHSSVDYWYMRNDIIAYFRN
                     NEQFAYDELVEKVRSHKPESPAIDERFDQIIEKFKALPDHKNSKFDRQFQLEPNVIRA
                     RIKKTIALSDNIELRINGEVADFRKKIVPESDALGKYLKIYSDTGYEEFSAGKEDNV"
     gene            652..>873
                     /locus_tag="RH057_004640"
     CDS             652..>873
                     /locus_tag="RH057_004640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130845.1"
                     /translation="MNIVSKIADLFMPSQEVSSCDNFHLFKAEYRILETTIHQDYSQR
                     VEELTNEVIANNLDIEIDFKFETCDSFTLL"
BASE COUNT          322 a          135 c          155 g          261 t
ORIGIN      
        1 ttttgatgag atagagtcaa aaatcaaaaa aggacttcct cttaatcgtc aaaggctcca
       61 aaaatcatgt ttagttagct tcagcaacac atatgatgtt gaagagattc ttatatcgga
      121 tagcggagct tcaattagtg aatattggtg gaaaaacttc ctcagtactg tagaacttca
      181 aagttctgaa cttaatacaa aaaatgcgtt tggttcaatt gaaaactttc ttaaaaggga
      241 agtagaaaag cactcttccg tggattattg gtatatgcgt aatgacatta ttgcttactt
      301 tagaaacaat gagcaatttg catacgatga attagtagag aaagtacgtt cacataagcc
      361 agaaagccca gctattgatg aacgtttcga ccaaataatc gagaaattta aagcattacc
      421 agatcataaa aactcgaaat ttgataggca atttcaatta gaaccaaatg taattcgtgc
      481 tagaataaag aaaacaatag ctttgtcaga caacattgaa ttaagaatca acggggaagt
      541 tgctgatttc cgtaaaaaaa tagttcccga atcagatgcg ttaggtaaat atctgaaaat
      601 ctactcagat actggttacg aagaatttag tgcaggaaaa gaagataacg tatgaatata
      661 gtatctaaaa tagccgattt attcatgcca tcgcaagaag tttcttcttg cgacaacttt
      721 catttattta aggctgaata tcgtatttta gaaactacta tccatcaaga ttattctcaa
      781 cgggtggaag aattaacaaa tgaagtcata gctaacaatc tagatattga aattgacttt
      841 aagtttgaga cttgtgattc attcaccctt ctt
//
LOCUS       DAPHDW010000358          868 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.366, whole genome shotgun sequence.
ACCESSION   DAPHDW010000358 DAPHDW010000000
VERSION     DAPHDW010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..868
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.366"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            49..681
                     /locus_tag="RH057_004641"
     CDS             49..681
                     /locus_tag="RH057_004641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3130846.1"
                     /translation="MGLCSRYKSLTCNSCSMHCQIMPEESPRLQYCANSCFCMWPEES
                     SYFNRGVVEGILTKNHNARLSGYIFVDFSVSFLRLFLEKDWIDYLASTDMGIVLVSDR
                     NMQSLANYWRKHNSAISAVIYNDDGLDVANEKIRQLFIGRYLSFTRGNTLTQMEFTIM
                     GYMVSGYNPYQIAEVLDMDIRSIYAYKQRIEKRMGGKINELFIRSHSVQH"
BASE COUNT          286 a          149 c          167 g          266 t
ORIGIN      
        1 atcaccaatg aaattatgca attatatacg gatagggagg ttcttaacat ggggctttgt
       61 agtcgttata aaagtcttac atgcaatagt tgctcaatgc attgccaaat aatgccagaa
      121 gagtcaccgc gtttgcagta ctgcgcaaac tcgtgttttt gtatgtggcc cgaagaaagt
      181 tcatatttta atcgtggtgt ggtagaaggt atcttaacaa aaaaccacaa tgcgagatta
      241 agcggatata tttttgtcga tttttcagta agttttttac gtctattcct tgaaaaagac
      301 tggattgact atcttgcaag tactgacatg gggattgttt tagtcagcga ccgcaatatg
      361 caatcgttag ctaattactg gcgaaaacac aactcagcaa tatctgctgt catatataat
      421 gatgatggtc ttgatgttgc caatgaaaag atcagacaac tatttattgg tcgttattta
      481 tcatttacca gaggtaacac attaactcag atggaattca ccataatggg gtatatggtt
      541 tctggttata atccatatca gattgctgaa gttctggata tggatatccg tagcatctac
      601 gcgtacaagc aacgaatcga aaagagaatg ggtggtaaaa taaacgaatt atttattcgt
      661 tcacattcgg tccaacattg atactcaacg accagccaga atcatactct gtttatacgg
      721 gcatttattg aaatgtccgt attttataaa taggccgtcc cctcaagagt aaacaccata
      781 gtatttaaaa ccatcacacg aaaaattcag aagcattacg aaatatgaat ttacaaacaa
      841 tagtggcata aatgttaacc atgttaat
//
LOCUS       DAPHDW010000359          855 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.367, whole genome shotgun sequence.
ACCESSION   DAPHDW010000359 DAPHDW010000000
VERSION     DAPHDW010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..855
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.367"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..364
                     /locus_tag="RH057_004642"
     CDS             <1..364
                     /locus_tag="RH057_004642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001767016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RNA-dependent DNA polymerase"
                     /protein_id="HDV3130847.1"
                     /translation="LSTELNLAYVARMLSKENSEKSTLILSEIYNNNPESILVKNIVT
                     QSMAKINSYAWLSDIKKNFSAMHPLQRRLLIVSSYILGDEGRHWREHNKKTFNFVEVI
                     YRDWASKRHTARNLEDAL"
     gene            361..>855
                     /locus_tag="RH057_004643"
     CDS             361..>855
                     /locus_tag="RH057_004643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130848.1"
                     /translation="MISELTFSRKFTSFWNQLLPNANNFIRIINGSLIEDVYPPLDDC
                     ANRSNNVFVNECAFNLYRAIQNDSLDRNILSAHDIFHNADFQVVFEKTKEYLQRFAYG
                     SNFKLPLSMVEYNAIREIARNILSRYGMENQIEVSPQFDGCGVINNSYGDIYYSNVLV
                     EIKSG"
BASE COUNT          294 a          150 c          158 g          253 t
ORIGIN      
        1 tttgagtact gagctcaact tagcctatgt agcccgaatg ctctcaaaag aaaattcaga
       61 aaaatccacc ctaatcctta gtgaaatata caataacaat ccagaaagca tcttagtcaa
      121 gaacatagtt acacagtcaa tggcaaaaat taattcttac gcatggcttt ctgatatcaa
      181 aaaaaatttc tctgcaatgc atccgttgca gagaagacta ttgatcgttt ccagttacat
      241 cttaggtgat gaaggacggc actggagaga gcataataag aaaacattca actttgtaga
      301 ggtgatttac agggattggg caagtaaaag gcataccgca agaaatcttg aggatgcgct
      361 atgatatctg aattaacgtt ttctcgaaaa ttcacttcat tttggaatca attgcttcca
      421 aatgctaata atttcatacg catcattaac ggcagtctca tcgaggacgt ttatcctcct
      481 ctagatgact gcgctaatag gtcaaataac gtctttgtta atgagtgcgc atttaattta
      541 tatagggcaa tacagaatga ttcgttagac agaaatattc tttcagcaca tgatatcttc
      601 cataatgctg attttcaggt tgtttttgaa aaaacaaaag aatatctaca gcggttcgct
      661 tacggttcta acttcaagct acccttaagc atggttgagt acaatgccat aagggaaata
      721 gcaagaaaca ttttgtctcg atatggaatg gaaaaccaaa ttgaagtgtc tccacaattc
      781 gatggatgcg gagtaataaa taattcatat ggcgatattt attattcaaa tgttcttgtg
      841 gaaataaaat cagga
//
LOCUS       DAPHDW010000360          854 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.368, whole genome shotgun sequence.
ACCESSION   DAPHDW010000360 DAPHDW010000000
VERSION     DAPHDW010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..854
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.368"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          261 a          166 c          159 g          268 t
ORIGIN      
        1 ggaccgcaca ggaaattact gctggtacga ccttaatgtc tgctacatca ttttgtcata
       61 caaaatattt ctttctgata cattctaagt tctatcacag cttcgccagt caatgttatt
      121 ttttctgtat ataagcttga aattctgtat gaaatggtgg gaaaatgata aggcacatag
      181 ggattagcct atttttgttt atgatcattg ccggcgtttt tcgccggctt ttttatgtct
      241 ccagagttat tacttttcga tattacggtg cttttcaact ggtctgcctg taaagccctg
      301 gaaaaaacga aaggtattta tgataaccga attttcgcga tgacgccctg accacattat
      361 gatttgagtc cggacatctc attgtcgtta tacaagaagc atgattatca atcacggaca
      421 ttacgacaat gcagcactgc ataccagaac tggatacctg cgggtccggc gacaactctg
      481 cctccctcat atataacacc agcacacgga cgataaagca gtacagggaa gaaatacagt
      541 gatatgtaac agcaagcctg ttaatgaaaa aagcctgcca tacggacata tggcaggtaa
      601 gtatttaagg gaataaccga agatgcgata gtgttgcatc cctcattcat agcccttaac
      661 aattatgcat caaaataaaa acatataaag gtattttgtt tcatgagtgt tgtttttgtg
      721 tgttatgtcg caaaactacc atgctgtatt atcctttaca tacttaacaa taaaattata
      781 tacattattt attatatgaa tatgatctgt ttctgataat aaatgtcctc cagaattaaa
      841 cgggctgccc ggat
//
LOCUS       DAPHDW010000361          851 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.369, whole genome shotgun sequence.
ACCESSION   DAPHDW010000361 DAPHDW010000000
VERSION     DAPHDW010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..851
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.369"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..487
                     /locus_tag="RH057_004644"
     CDS             <1..487
                     /locus_tag="RH057_004644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130849.1"
                     /translation="SPQLLGLKDNVYFCKIDSSQMLFPNQVGVGLTQIAPLIIAANIV
                     QDGLIAIEQPELHIHPALQLAVGDLFTQYPLDVKRPMFLVETHSEHILLRILKRIRQT
                     TDNELPESNYPVKPDFISVIVFEDNNGSTVTRKIDITDDGDFKQKWPKGFFEERRGEL
                     F"
     gene            487..>851
                     /locus_tag="RH057_004645"
     CDS             487..>851
                     /locus_tag="RH057_004645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001680722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130850.1"
                     /translation="MFYECAISPEALFEIAIDRRNYRDFIKGFSTGGNFLYSELPKLK
                     RNKKQLLGLLNANHSELQKKRLEDLIIFLKNNKVSRVYDYVGDMSWSDNISAVNRIEQ
                     FDHVVSSTPCDNLDVTNID"
BASE COUNT          282 a          139 c          159 g          271 t
ORIGIN      
        1 ctcccctcaa cttttaggtc tgaaagataa tgtttacttc tgtaaaatag attcaagcca
       61 aatgttattt ccaaatcaag tcggtgtcgg attaactcag atagcacctc ttattattgc
      121 agccaatata gttcaggatg gattaattgc tatagagcag cctgagctac atattcatcc
      181 cgctttacaa ctcgctgtag gtgatctgtt tacccaatat ccccttgatg ttaaaagacc
      241 tatgttttta gtcgaaacac atagtgagca cattttgctt cgaattttaa aaagaattag
      301 acaaacaacg gacaatgaat tacctgaatc aaattaccca gtaaaacctg attttatatc
      361 agtgatcgtt ttcgaggata ataatggttc gactgttact aggaaaatag atattactga
      421 tgatggtgac ttcaaacaaa aatggcccaa aggtttcttc gaagaaagaa ggggagagct
      481 tttttaatgt tctacgaatg cgccatttca ccagaggctt tgtttgagat cgcaattgat
      541 cggagaaatt atcgtgactt cataaaagga ttttctacgg gcggtaactt tctctatagt
      601 gaattaccaa agctgaagag aaataaaaaa caattgcttg ggctattaaa tgctaatcat
      661 agtgaacttc aaaaaaaacg attagaggac cttattattt ttttgaaaaa taataaggtt
      721 agtagggttt atgattatgt tggtgacatg tcatggtcag ataacatctc tgctgtaaat
      781 cgaattgaac aatttgatca tgtagtgagt tctactcctt gtgataacct tgatgtaact
      841 aatatagatg a
//
LOCUS       DAPHDW010000362          848 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.370, whole genome shotgun sequence.
ACCESSION   DAPHDW010000362 DAPHDW010000000
VERSION     DAPHDW010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..848
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.370"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            196..357
                     /locus_tag="RH057_004646"
     CDS             196..357
                     /locus_tag="RH057_004646"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130851.1"
                     /translation="MQKNVTPGRRKGCPNYPPEFKQLLVAASCEPGISISKLALENGI
                     NANLLFKWR"
     gene            573..848
                     /gene="tnpB"
                     /locus_tag="RH057_004647"
                     /pseudo
     CDS             573..848
                     /gene="tnpB"
                     /locus_tag="RH057_004647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
BASE COUNT          203 a          231 c          224 g          190 t
ORIGIN      
        1 catctggtag cctctccggg ttgccattgt ggcgatgctt cttgctccgg ccgaaccgtg
       61 gctgatgcca tgtagctcaa gtacctgact gcgtaataca gccgtaagcg tacagcctga
      121 accgtctggt cagaatctga caaattagac aaagtggtgt ccaccaaata agtagtggga
      181 accaaagtgt cagatatgca gaaaaatgtg actcccggca ggcgaaaagg ctgccctaat
      241 tatcctcctg aatttaaaca gctgctcgtt gctgcctcct gtgaacccgg gatatccatc
      301 tcaaaacttg ctcttgaaaa tggcattaac gccaatctgt tgttcaaatg gcgataacaa
      361 tggcgcgagg gaaagctgct attaccttct tcagagagcc cccagctact tcctgtgact
      421 ctcgatgcag ctgccgaaca gccagaatcg ctcgcagagg acccggaaac cctcagtatc
      481 agctgtgagg taacgttccg gcacgggacg ctccgcttca atggcaatgt cagcgaaaag
      541 ctcctgactc tgctgataca ggaactgaag cgatgatccc gttaccttcc gggaccaaaa
      601 tttggctggt tgccggtatc accgatatga gaaatggctt caacggcctg gctgcgaaag
      661 tatagacggc gctgaaagac gatcccatgt ccggccatgt tttcattttc cggggccgca
      721 gcggcagtca ggttaaactg ctgtggtcca ccggtgacgg actgtgcctc ctgaccaaac
      781 ggctggagcg tgggcgcttc gcctggccgt cagcccgtga tggcaaagtg ttccttacac
      841 aggcgcag
//
LOCUS       DAPHDW010000363          844 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.371, whole genome shotgun sequence.
ACCESSION   DAPHDW010000363 DAPHDW010000000
VERSION     DAPHDW010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 844)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..844
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.371"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..687
                     /locus_tag="RH057_004648"
     CDS             <1..687
                     /locus_tag="RH057_004648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001339197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HDV3130852.1"
                     /translation="LGWYWLSRVRGKVQYADLGAENWKPISNLHDMSSSHSKTLGYKR
                     LTKSNPISCQILLYKSRSKGRKNQRSTRTHCHHPSPKIYSASAKEPWILATNLPVEIR
                     TPKQLVNIYSKRMQIEETFRDLKSPAYGLGLRHSRTSSSERFDIMLLIALMLQLTCWL
                     AGVHAQKQGWDKHFQANTVRNRNVLSTVRLGMEVLRHSGYTITREDSLVAATLLTQNL
                     FTHGYVLGKL"
     gene            complement(703..822)
                     /locus_tag="RH057_004649"
                     /pseudo
     CDS             complement(703..822)
                     /locus_tag="RH057_004649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227464.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
BASE COUNT          259 a          196 c          178 g          211 t
ORIGIN      
        1 ctgggttggt actggttaag tcgagtaaga ggaaaagtac aatatgcaga cctaggagcg
       61 gaaaactgga aacctatcag caacttacat gatatgtcat ctagtcactc aaagacttta
      121 ggctataaga ggctgactaa aagcaatcca atctcatgcc aaattctatt gtataaatct
      181 cgctctaaag gccgaaaaaa tcagcgctcg acacggactc attgtcacca cccgtcacct
      241 aaaatctact cagcgtcggc aaaggagcca tggattctag caactaactt acctgttgaa
      301 attcgaacac ccaaacaact tgttaatatc tattcgaagc gaatgcagat tgaagaaacc
      361 ttccgagact tgaaaagtcc tgcctacgga ctaggcctac gccatagccg aacgagcagc
      421 tcagagcgtt ttgatatcat gctgctaatc gccctgatgc ttcaactaac atgttggctt
      481 gcgggcgttc atgctcagaa acaaggttgg gacaagcact tccaggctaa cacagtcaga
      541 aatcgaaacg tactctcaac agttcgctta ggcatggaag ttttgcggca ttctggctac
      601 acaataacaa gggaagactc actcgtggct gcaaccctgc ttactcaaaa tctattcaca
      661 catggttacg ttttggggaa attatgaggg gatctctcag tgctcagcat gtttgagtgc
      721 tttactcgcc gcgggttgag aaatatgtaa aacttcagcc gcacgcgtta aggaaccaca
      781 agtcattaca gcataaaaga tatcgagatg tcttaatttc attccatgta gcctactgat
      841 tatt
//
LOCUS       DAPHDW010000364          836 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.372, whole genome shotgun sequence.
ACCESSION   DAPHDW010000364 DAPHDW010000000
VERSION     DAPHDW010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.372"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(135..698)
                     /gene="ipbA"
                     /locus_tag="RH057_004650"
                     /gene_synonym="fimX"
     CDS             complement(135..698)
                     /gene="ipbA"
                     /locus_tag="RH057_004650"
                     /gene_synonym="fimX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_060710320.1"
                     /note="IpbA is also known as HbiF and FimX; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase IpbA"
                     /protein_id="HDV3130853.1"
                     /translation="MTRKYLTQDEVYRLMDAAQSMSFPERNRCLIMMAFIHGFRASEL
                     LDLRLSDIDASGKQLNIRRIKNGFSTTHPLLPDEYNLIKLWLKQRKLIENGVEGDWLF
                     LSRKRRPISRQHFFSIIREAGKRAGLAVKAHPHMLRHACGFALADNGVDTRLLQDYLG
                     HRNIQHTVRYTASNAARFKGVWKKKPR"
BASE COUNT          262 a          159 c          163 g          252 t
ORIGIN      
        1 aaatcatggg gttattttac tttagcgaga aatatcaaca tgttatgtgt tttatttaat
       61 tgaatgggct gtttttttat ggaagaattg aggaaaacgt aggaatctga cattttgaat
      121 cagaaggtac tgttttaacg aggctttttt ttccacaccc ctttaaaacg agcagcatta
      181 ctggctgtgt atctgacagt atgctgaata tttcgatgac ccagataatc ctgaagtagt
      241 cgggtatcga caccattgtc agccagagca aaaccacagg catgacgtaa catatgagga
      301 tgtgctttta cagctaatcc tgcacgtttt ccagcctcac gaatgataga aaaaaaatgt
      361 tgtctgctga tagggcggcg tttccgggag agaaaaagcc agtctccttc aactccgttt
      421 tcaattaact tcctctgctt aagccacaac ttgatcaaat tatattcatc aggaagaaga
      481 ggatgtgttg tcgagaaacc atttttaata cgccgaatat taagttgttt tccagatgca
      541 tcaatatcgg ataaacgtaa atcaagaagt tcactggccc taaaaccgtg aatgaaagcc
      601 atcataatta gacagcgatt tctttcagga aaggacatac tctgggctgc atccatcagc
      661 ctgtagactt catcctgtgt gagatatttt ctcgtcatac gccaccgtgt acagttttaa
      721 aaaaaagaga ggcacaccca gataagtata cctgcaatca gaacgttatc gatgtaatct
      781 cattatctca ttattaagat tttttgaata gtagttctac gcaacagatt aaatat
//
LOCUS       DAPHDW010000365          836 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.373, whole genome shotgun sequence.
ACCESSION   DAPHDW010000365 DAPHDW010000000
VERSION     DAPHDW010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 836)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..836
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.373"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>836)
                     /locus_tag="RH057_004651"
     CDS             complement(<1..>836)
                     /locus_tag="RH057_004651"
                     /inference="COORDINATES: protein
                     motif:HMM:NF027494.1,HMM:TIGR04415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV3130854.1"
                     /translation="GGSAVDTTIEEGLQTVLNGGNVSGTHISGGEQRVSSGGSAVDTT
                     IEEGFQTVLNGGNVSGTHISGGEQNISSGGSAVDTTIEVGLQTVFDGGSVNGTIIDGG
                     EQHISSGGSAINTTLDGYQTVFNGGNATGTTINGGFQNISSGGSATSTIINAGFQEVS
                     SGGSATSTTINAGFQALYDSSIASGTVINNGFQLISSGGSAINTTIKGGFQEVSDGGR
                     AIETTITSGWQNVLSGGVATETLIVGGVQTIYDGGSASEITINSGYQVISSGGSVTTT
                     TI"
BASE COUNT          193 a          248 c          188 g          207 t
ORIGIN      
        1 taaatggtcg tggtggttac actgccaccg cttgagatga cctggtaacc actgttgatt
       61 gtaatctccg acgctgagcc tccgtcgtaa atcgtctgga ctcccccgac aatgagcgtc
      121 tcagttgcaa ccccaccact taatacattc tgccagccac tagtaattgt ggtctcaata
      181 gctctgccac catcgctaac ctcctggaat ccccctttaa tggtggtgtt gatggcagag
      241 ccaccactgg aaatgagctg gaagccatta ttaatgacgg tgccgctggc tatactgcta
      301 tcgtagagag cctggaaccc ggcattaata gtggtgctgg tagcactgcc accactggaa
      361 acctcctgga atccggcatt aataatggtg ctggtagcac tgccaccact ggaaatattc
      421 tggaacccgc cattaattgt ggtgcctgtt gcgttgccac cattgaaaac tgtctgatag
      481 ccatccagtg tggtgttaat cgcacttccc ccactggaaa tgtgctgctc cccaccatca
      541 atgatcgtgc cattaacact tccgccatca aaaacagtct gtagtcccac ctcaatggtg
      601 gtgtctacag cactaccacc gctggaaatg ttctgctcac ctccgctaat gtgagtcccg
      661 gagacatttc cgccattgag aacagtctga aacccctcct caatggtggt gtctacagca
      721 ctaccaccgc tggaaactcg ctgctctcct ccgctaatgt gagtcccgga gacatttccg
      781 ccattgagaa cagtctgaag cccctcctca atggtggtgt caacggcact gccgcc
//
LOCUS       DAPHDW010000366          830 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.374, whole genome shotgun sequence.
ACCESSION   DAPHDW010000366 DAPHDW010000000
VERSION     DAPHDW010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.374"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            39..>830
                     /locus_tag="RH057_004652"
     CDS             39..>830
                     /locus_tag="RH057_004652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3130855.1"
                     /translation="MYWIINDNIEFWPEHRKLISVHNADLNVVLTTPASRCLSLLLEA
                     FPDVVAQQDFFTRVWEEEGMRVPTNTLYQNISIIRRGFRAVGDTTQSLIATVPRRGFK
                     IHNDINIQNHVINSSTDAHTHNAPPAIKVNAGYKESIGGAKNFNNKILKHIKSHLIML
                     SAFVIGAYSAYWLWNNNQPKPFFKDYKTVAEINGCHFNVTEDTIDGLKEFDKYKTRIL
                     DSGINCKKHPWLYFPLAKSSPGMIVMACNKNYNQHEVADCLTLSYR"
BASE COUNT          270 a          164 c          163 g          233 t
ORIGIN      
        1 gtgatctcca tttcgattga tttagtgttt attgacgtat gtactggatt attaacgata
       61 atatcgagtt ctggcctgag caccgaaaat taatatcggt acataacgcc gatcttaacg
      121 tcgttctgac aacgccagcc agtcgatgtt tatcacttct acttgaagct tttcctgatg
      181 tggttgcaca acaagatttt ttcaccagag tctgggaaga agaaggtatg cgtgtgccta
      241 ctaacacgtt atatcagaac atatccatta tcagacgcgg atttcgcgct gttggtgata
      301 ctacccagtc gctaattgca accgtgccga gaagaggatt caagatccat aatgacatta
      361 acatacaaaa tcatgtaata aactcgtcaa cagacgcaca tacacacaat gccccacctg
      421 ccataaaagt taatgcggga tacaaagaga gcattggtgg cgcaaagaat ttcaataaca
      481 aaatcctcaa acatataaaa agtcatctaa ttatgttgag cgcatttgtc ataggcgcat
      541 attctgcata ttggctatgg aataataatc aaccgaagcc atttttcaag gattacaaga
      601 ctgttgcgga aattaatggc tgccatttta atgtaacaga agatacaatt gacgggttga
      661 aagagttcga taaatataaa acacggatac tggattcggg gattaactgt aaaaaacatc
      721 cgtggttata ctttcctctt gcaaaatcct caccggggat gattgttatg gcatgcaata
      781 aaaattataa ccaacatgaa gtggcagact gtttaaccct gtcttaccgt
//
LOCUS       DAPHDW010000367          823 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.375, whole genome shotgun sequence.
ACCESSION   DAPHDW010000367 DAPHDW010000000
VERSION     DAPHDW010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..823
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.375"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..263
                     /locus_tag="RH057_004653"
     CDS             <1..263
                     /locus_tag="RH057_004653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HDV3130856.1"
                     /translation="SNAIASTPVYDNYFNNTTAFRPKPEDIFKCGQTLKKTKQKYGHY
                     GLGVDQHQKIDNYTMGLNSWKIAPNERGDKKGVPGLSCFQPH"
     gene            525..>823
                     /locus_tag="RH057_004654"
     CDS             525..>823
                     /locus_tag="RH057_004654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130857.1"
                     /translation="MKIGTVAGTNDSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIRDDKISFTIQTNHSEKYSMLSDMRAHIL"
BASE COUNT          283 a          137 c          159 g          244 t
ORIGIN      
        1 gaagcaatgc tatagcatcc acacctgtat atgacaatta ttttaataac actactgctt
       61 tcagaccaaa acctgaagat atcttcaaat gtggacagac gctcaaaaaa actaaacaga
      121 aatatggcca ttatggatta ggggtagatc agcatcaaaa aattgataat tataccatgg
      181 ggctaaattc ctggaagata gctcctaacg aacgtggtga taaaaaaggt gtcccagggc
      241 ttagttgttt tcaaccacat taaaactcca tgagtgctgt ggtatatctt tttaatcctg
      301 ccagtatttc ataggttttc ttaatcgatt gtagaggcat gttctgatga cgcctctaca
      361 atctttaggg actttctctg ctatgagata agaaatttca tattatgctt taaggctgaa
      421 tgatttgtgc aaaatataat tatttttcca tcgaacatga ggtgtatgtc ataagattaa
      481 atgttaacta tgcattcctc gcggtgaatt atatgaggta aaagatgaaa attggaactg
      541 tggcaggcac taacgacagt acaacgacaa tcgcaacaaa tgatatggtg caagagcatg
      601 ttactaactt cactaaagaa ctgtttgggt atattgcaaa tggtataggt gatgatatat
      661 caagtattgc caggacaatg ctcggtgaag ttgtggaaaa aattgatgat tggcaaatag
      721 aacgtttcca acaatctatt cgagatgaca aaatatcatt taccatacaa acgaatcatt
      781 cagaaaaata tagtatgcta tcggacatgc gcgctcatat tct
//
LOCUS       DAPHDW010000368          803 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.376, whole genome shotgun sequence.
ACCESSION   DAPHDW010000368 DAPHDW010000000
VERSION     DAPHDW010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..803
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.376"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            6..>803
                     /locus_tag="RH057_004655"
     CDS             6..>803
                     /locus_tag="RH057_004655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HDV3130858.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNRHPRITDNSTYEQIMAEEFTQLISKSAGVDDIQMEIDER
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNSYRDFLNLKEIKL"
BASE COUNT          252 a          175 c          145 g          231 t
ORIGIN      
        1 agtgtatggc caatctctac gacttgaaaa agttcgactt aaaccttctt gtcatatttg
       61 agtgtattta ccagcattta agcattagca aagctgctga atcactatat atcaccccat
      121 cagccgtgag ccagtcgtta caacgcttac gtgcgcaatt taatgaccct ttattcattc
      181 gctcaggaaa aggtatcgcg ccgacgacaa cagggttgaa tctgcatcac catcttgaga
      241 aaaacctcaa aggccttgaa caaacaatta atatagtcaa taagtccgaa ctaaagaaaa
      301 actttattat ttacggccct cagctcattt cttgctctaa taatagtatg ctgatccgct
      361 gcctacggca agatgcctct gtcgagattg agtgccatga tatcctcatg tcagcagaga
      421 atgctgaaga actgttggtt caccgcaaag ccgatctggt gatcacccaa atgccagtga
      481 tcagccgttc cgtcatttgc atgccattgc atacgattcg caatactcta atttgtagta
      541 ataggcatcc acgaattact gacaatagca cttatgaaca aattatggca gaagagttca
      601 cccaattaat atctaaatca gcaggggttg atgatattca aatggaaata gacgagagat
      661 ttatgaatcg caaaatctca tttcgtggtt cctctttgct gacaatcatt aatagcattg
      721 cagttacaga tttactggga atagtcccct atgaacttta taattcctac cgtgactttc
      781 ttaatttaaa agagataaaa cta
//
LOCUS       DAPHDW010000369          800 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.377, whole genome shotgun sequence.
ACCESSION   DAPHDW010000369 DAPHDW010000000
VERSION     DAPHDW010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..800
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.377"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(34..561)
                     /locus_tag="RH057_004656"
                     /pseudo
     CDS             complement(34..561)
                     /locus_tag="RH057_004656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014641070.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            619..798
                     /locus_tag="RH057_004657"
     CDS             619..798
                     /locus_tag="RH057_004657"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130859.1"
                     /translation="MRKLLSATGAELTNGVTLPSGWLVSGMSKGANNPENLRAFAVCM
                     RQWQHKNSRINFCLN"
BASE COUNT          218 a          172 c          177 g          233 t
ORIGIN      
        1 taacccatca ggggagagtt atgcggcaat aacttagcat ttttgagacg caacatagtc
       61 actccaccat tgcatgagaa ccacccgttc tgtgggatat tctgcacgat tgtaggctgc
      121 aattatttca tctttcttcg agtgggtaag ggcggcttcg aagacatcag tcctaaactt
      181 tccagactca tcagcagcat cgcataccgt gagctacaag cttacctccg aaccccatac
      241 ggattatggc cgcattagct gtttgctcat gcatatggtt aagaggagcc tttatgcttg
      301 gaaacaccca ttcacgatgc ccacttatca ctttcattga ctctaggact cgtaaagctt
      361 ctttgcttag tggaactttg tgaggcttct tcattttcat aaactcagcc gggatgttcc
      421 acataccagt ttctatatca atatctgtcc atcttgtgcg aaccgcttca gcgggcgaac
      481 ccatgttaga agttgccact caatcagtag ccttgtttct aaccggatag aagcattgtt
      541 tagagcaact aggaagcggg gggattactg tttctggtaa agactctttt ggtggtgaac
      601 tggacgcctg gaatcaatat gagaaaacta cttagtgcaa caggagctga attaactaat
      661 ggtgtaacat tacctagtgg ttggcttgtc agtggtatga gcaagggagc caataatcct
      721 gaaaatttaa gagcgtttgc tgtttgtatg agacaatggc agcataaaaa ttctaggata
      781 aacttttgcc ttaattaaaa
//
LOCUS       DAPHDW010000370          790 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.378, whole genome shotgun sequence.
ACCESSION   DAPHDW010000370 DAPHDW010000000
VERSION     DAPHDW010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..790
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.378"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..790
                     /locus_tag="RH057_004658"
                     /pseudo
     CDS             1..790
                     /locus_tag="RH057_004658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          184 a          206 c          238 g          162 t
ORIGIN      
        1 tggcaaaacg ggatgaagag ctggctatcc tccaaaaggc cgcgacatac ttcgcgaagc
       61 gcctgaaatg aagtatgtct ttattgaaaa acatcaggct gagttcagca tcaaagcaat
      121 gtgccgcgtg ctccgggtgg cccgcagcgg ctggtatacg tggtgtcagc ggcggacaag
      181 gataagcacg cgtcagcagt tccgccaaca ctgcgaaagc gttgtcctcg cggcttttac
      241 ccggtcaaaa cagcgttacg gtgccccacg cctgacggat gaactgcgtg ctcagggtta
      301 cccctttaac gtaaaaaccg tggcggcaag cctgcgccgt cagggactga ggacaaaggc
      361 ctcccggaag ttcagcccgg tcagctaccg cgcacacggc ctgcctgtgt cagaaaatct
      421 gttggagcag gatttttacg ccagtggccc gaaccagaag tgggcaggag acatcacgta
      481 cttacgtaca gatgaaggct ggctgtatct ggcagtggtc attgacctgt ggtcacgtgc
      541 cgttattggc tggtcaatgt cgccacgcat gacggcgcaa ctggcctgcg atgccctgca
      601 gatggcgctg tggcggcgta agaggccccg gaacgttatc gttcacacgg accgtggagg
      661 ccagtactgt tcagcagatt atcaggcgca actgaagcgg cataatctgc gtggaagtat
      721 gagcgcaaaa ggttgctgct acgataatgc ctgcgtggaa agcttctttc attcgctgaa
      781 agtggaatgt
//
LOCUS       DAPHDW010000371          787 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.379, whole genome shotgun sequence.
ACCESSION   DAPHDW010000371 DAPHDW010000000
VERSION     DAPHDW010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..787
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.379"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..291
                     /locus_tag="RH057_004659"
     CDS             <1..291
                     /locus_tag="RH057_004659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HDV3130860.1"
                     /translation="LDQHYGLTLNDTPFSDERVIEQHIEAGISLCDAVNFLVEKYALV
                     RTDQPGFSAGTSSQLINSIDILRARRATGLMTRDNYRTVNNIALGKHPGAKQ"
     gene            288..776
                     /locus_tag="RH057_004660"
     CDS             288..776
                     /locus_tag="RH057_004660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130861.1"
                     /translation="MKLALKLEADSINVQALNMGRIVVDIDGVNLAELINVACDNGYS
                     LRVVDESDRTSADCTPPFAALTGIRCSTAHITAKDNAWLYSLSHQTNDTGESEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSKTFRRLFVTLIRRYGVSLIHLDAGAECLPGLPTF
                     NW"
BASE COUNT          189 a          214 c          202 g          182 t
ORIGIN      
        1 ctggaccagc attatggcct cacactgaat gacacaccgt tttccgatga acgtgtgatt
       61 gagcagcata ttgaggcagg tatttcactg tgtgatgcgg tgaactttct cgttgaaaaa
      121 tacgccctgg tacgtaccga ccagccggga ttcagcgcag gaacttcgtc gcagttaatc
      181 aacagcattg atattctccg ggcacgccgg gcaaccggcc tgatgacccg cgataactac
      241 agaacggtaa ataacattgc cctgggtaaa catccggggg cgaaacaatg aaactggccc
      301 tgaaactgga agccgacagc attaacgtgc aggcactgaa catggggcgc attgtcgttg
      361 acattgatgg tgttaatctc gctgaactga ttaacgtggc ctgcgacaat ggctactccc
      421 ttcgtgttgt tgatgaatct gaccggacct cagcagactg cacgccacca tttgctgccc
      481 tgaccggcat acgctgcagt accgcacata tcacggcaaa ggacaacgcc tggctgtact
      541 cgctgtcgca tcagacgaat gacaccggcg aatcagaatg gattcatttt acaggcagcg
      601 gatatctgtt acgtaccgat gcgtggtcat acccggttct gcggcttaag cgcctgggcc
      661 tgtcaaaaac gttccgccgt ctgtttgtca cactcatccg acgttatggc gtcagtctca
      721 ttcatctgga tgccggcgct gaatgcctgc cgggtttacc cactttcaac tggtaaccag
      781 gagcaac
//
LOCUS       DAPHDW010000372          771 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.380, whole genome shotgun sequence.
ACCESSION   DAPHDW010000372 DAPHDW010000000
VERSION     DAPHDW010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.380"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          188 a          154 c          170 g          259 t
ORIGIN      
        1 cctgtggcgt atgcatgatg caaggttgaa cgcatgtaat atgatattta tcacgtaaat
       61 gcagtgtaat cttcttgcat tgtttttagg ttgctattcc gtttatctga ggactgctgg
      121 ctattgttgt gcagttttcc tgatacagct ttatgcaggt tttccagcat tacagtaaaa
      181 cccgggaaca tcagtgctgc tcgtttcttt ctcctgtatc cactcccgct tatagatact
      241 tccttaaata ttctctcgtc tgactaaagc atatataccc gttcatggga tgttggctgt
      301 aatgattttc ctgagcggat tattactgat gaccataagt atgtctcagc acagtcatcg
      361 taacgtgctg gttaacctgg ccatggtagc caaatcatca tcctgtatat tctgttgagg
      421 gaggcactgg tgattgttca gaatacggct gatgcgaaaa agtgcaggct gtttccttta
      481 ttggtgtgat gtacagcctc tttacgctct acttttccgg ggaagaagcc atgctgtagc
      541 actttattgt tacttttcct ggctggacac tgtatggtct ggtatccccc cacgttttga
      601 acggaaaaaa cagatttaaa gttgccagta gtctgtaagt ttctgcatcc catttcccca
      661 cagacagtat tctcctgcaa tgtgatgaat ggtttcctgg aacaggaaag gaactaagtc
      721 agatagtgga gaaagtggtt aacacgttgt ttttaattaa aattttggta a
//
LOCUS       DAPHDW010000373          743 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.381, whole genome shotgun sequence.
ACCESSION   DAPHDW010000373 DAPHDW010000000
VERSION     DAPHDW010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 743)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 743)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..743
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.381"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            65..346
                     /locus_tag="RH057_004661"
                     /pseudo
     CDS             65..346
                     /locus_tag="RH057_004661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001434876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS domain-containing protein"
     gene            434..>743
                     /locus_tag="RH057_004662"
     CDS             434..>743
                     /locus_tag="RH057_004662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001448399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130862.1"
                     /translation="MKRLNLGGTDQFFHCMAFCRVSKLNDAGVSRSAKGLGYEKEIRD
                     YGLNMFGMYGRKVKLSHSEMIEDNKKDLAVNEHGLTCPLTQDCSNRCIDYINPEHKKT
                     I"
BASE COUNT          239 a          113 c          197 g          194 t
ORIGIN      
        1 gtgtatttat tatagaaatt aataagaaaa atggatgtgt tttgaatttc ctacatagta
       61 aataatgctg gcgctgatgg atgcggatgg aaacattgcg tggagcgggg agtatgatga
      121 gtggggcaac cagctgaatg aagagaaccc gcatcacctg caccagccct accggctgcc
      181 ggggcagcag tatgataagg agtcggggct gtactacaac cggaaccggt actacgatcc
      241 gttgcagggg cggtatatca ctcaggaccc gatagggctg gaggggggat ggagtctgta
      301 tgcgtatccg ctgaatccgg tgaatggtat tgatccatta gggttaagtc ctgcagatgt
      361 agcgttaatg cgaaaaaaag aacaactaaa ccatcaaaga gcatgggata tattatctga
      421 tacttatgat gatatgaaga gattaaattt aggtgggact gatcaatttt tccattgtat
      481 ggcattttgt cgagtgtcta aattaaatga cgctggtgtt agccgatcag cgaaagggct
      541 gggttatgaa aaagagatta gagattacgg gttaaatatg ttcggtatgt acggcagaaa
      601 agtaaagtta tcccattctg agatgattga agataataaa aaagacttgg ctgtaaatga
      661 acatgggttg acatgtccat taacacaaga ttgctcaaat agatgtattg attatattaa
      721 tccagagcat aaaaaaacga taa
//
LOCUS       DAPHDW010000374          740 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.382, whole genome shotgun sequence.
ACCESSION   DAPHDW010000374 DAPHDW010000000
VERSION     DAPHDW010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.382"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>740
                     /locus_tag="RH057_004663"
     CDS             <1..>740
                     /locus_tag="RH057_004663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HDV3130863.1"
                     /translation="IVIVTGESVRRDYMSVYGYPVPTTPWLNTAPGLFIDGYTSAAAS
                     TVPSLSRTLIYDYEQNPDSGNNVVALAAKSGYSTWWISNQGKLGEHDTRISVIASDAE
                     HTVFLKKGSFASRKTDDMLLLQETERALADTSSPKVIFLHMIGSHPNPCDRLNSWPNH
                     YLEQYPRKIACYLASISKLDNFLGQLDGILRRHSRHFAMLYFSDHGLSVSDSANPVHH
                     DGHVQGGYSVPLIITASDITSHQSVSRK"
BASE COUNT          174 a          191 c          179 g          196 t
ORIGIN      
        1 attgttatcg ttactggtga gagtgtgcgc agggattata tgtcggtata tggttatccc
       61 gtgccaacca caccgtggct gaatacggct cccggtttat ttattgacgg ctatacatcg
      121 gcagcagcca gtaccgtacc ttccctgagc cggacactga tttatgacta tgagcagaac
      181 cctgattccg gcaacaacgt ggtggcgctg gcagcaaaat caggatacag cacatggtgg
      241 atatccaatc agggaaaact gggagagcat gacacacgca tctctgttat tgcttctgat
      301 gcggagcata ccgttttcct caagaaaggc agcttcgctt cccgtaaaac ggatgatatg
      361 ttgttgttac aggaaacaga acgtgcgctg gcggatacat cctctccgaa ggtgattttc
      421 ctgcacatga ttggctctca tccgaatccg tgtgaccgac ttaactcctg gccgaatcat
      481 tacctggagc agtatccccg aaagattgcc tgttacctcg ccagcatcag taaactggat
      541 aactttctcg gtcagcttga tggtatcctt cgccggcatt cccgtcattt cgccatgctt
      601 tacttttctg accatgggct gtcggtcagc gacagtgcta atcctgttca tcatgatggt
      661 catgtgcagg gaggctacag cgttcccctg attattaccg ccagtgacat cacgtctcat
      721 cagtccgtca gcagaaaaat
//
LOCUS       DAPHDW010000375          733 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.383, whole genome shotgun sequence.
ACCESSION   DAPHDW010000375 DAPHDW010000000
VERSION     DAPHDW010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.383"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            46..>733
                     /locus_tag="RH057_004664"
     CDS             46..>733
                     /locus_tag="RH057_004664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HDV3130864.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPTDLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQ"
BASE COUNT          243 a          127 c          163 g          200 t
ORIGIN      
        1 acgatttacc agtaatgtaa ataaattttc gaggagatca ttccagtggg acgtaaatgg
       61 gccaatattg ttgctaaaaa aacggctaaa gacggtgcaa cgtctaaaat ttatgcaaaa
      121 ttcggtgtag aaatctatgc tgctgctaaa caaggtgaac ccgatccaga attaaacaca
      181 tctttaaaat tcgttattga acgtgcaaag caggcacaag ttccaaagca cgttattgat
      241 aaagcaattg ataaagccaa aggcggcgga gatgaaacgt tcgtgcaggg acgttatgaa
      301 ggctttggtc ctaatggctc aatgattatc gccgaaacat tgacttcaaa tgttaaccgt
      361 acgattgcta acgttcgcac aattttcaat aaaaaaggcg gcaatatcgg agcggcaggt
      421 tctgtcagct atatgtttga caatacgggt gtgattgtat ttaaagggac agaccctgac
      481 catatttttg aaattttact tgaagctgaa gttgatgttc gtgatgtgac tgaagaagaa
      541 ggtaacattg ttatttatac tgaacctact gaccttcata aaggaatcgc ggctctaaaa
      601 gcagctggaa tcactgagtt ctcaacaaca gaattagaaa tgattgctca atctgaagtt
      661 gagctttccc cagaagattt agaaatcttt gaagggcttg ttgatgccct tgaagatgac
      721 gacgatgtac aaa
//
LOCUS       DAPHDW010000376          732 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.384, whole genome shotgun sequence.
ACCESSION   DAPHDW010000376 DAPHDW010000000
VERSION     DAPHDW010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 732)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 732)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..732
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.384"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(1..732)
                     /locus_tag="RH057_004665"
                     /pseudo
     CDS             complement(1..732)
                     /locus_tag="RH057_004665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000420980.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
BASE COUNT          206 a          169 c          121 g          236 t
ORIGIN      
        1 gatataatat ctgaacgtca tttctggctc tttcttttgt tctgctatta ttgaccgaaa
       61 ggagactgcc acgcagaatt ttttcagccc tttccattca aacgttaaat caataagttc
      121 atcagggaca tcgcaaacaa tataaagacg gatttcttct ctgccgtgac tcttttcact
      181 cattgcgtaa ctgtcatgct ctggattatt taattctttc agcggaaatt tttcctgaaa
      241 ggctttatta agcccccctg gtttccgtgt acagcgaata aataatcacc tccctgtttt
      301 tgtatcttct cagcaatatc tttcaggcaa cccatcgcat cagttgtgat aatttttcct
      361 ttaatatcca acgtgttaat aagctcaggg atagctgtaa tctcattgga tttctcatct
      421 gtcctgaacc agactgtgca ttgttaagaa cgcactaatg acatgaatca ctcccttgcg
      481 acgactcttg tcataagatt gccggagtgt tttccatcaa ttgcgatgac gtctttatca
      541 tttgaagaat ggcatccacg catccagtta ataaggcact cgtaaaattt tgcaggactg
      601 atacaggata taactctggc aatggtaccg tgaacaggaa taccattttc aaaatcacca
      661 tattgcttca aaaaatcgag atgagtttcc caaaaaccct atatcttccc agccttctgc
      721 accagaaata ac
//
LOCUS       DAPHDW010000377          731 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.385, whole genome shotgun sequence.
ACCESSION   DAPHDW010000377 DAPHDW010000000
VERSION     DAPHDW010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.385"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..237
                     /locus_tag="RH057_004666"
     CDS             <1..237
                     /locus_tag="RH057_004666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HDV3130865.1"
                     /translation="AHAAEVYNKDGNKLDLYGKVTALRYFTDDKRDDGDKTYARLGFK
                     GETQINDQMIGFGHWEYDFKGYNDEANGSRGNNL"
     gene            261..>731
                     /locus_tag="RH057_004667"
     CDS             261..>731
                     /locus_tag="RH057_004667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HDV3130866.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFNATNYDRLVKLSLNSNTLESINFPQGRNVSITHI"
BASE COUNT          266 a          154 c          116 g          195 t
ORIGIN      
        1 gcacacgctg ccgaagtcta taacaaggat ggtaataaac tcgaccttta tggcaaggtt
       61 accgctctac gttattttac tgatgataag cgtgacgatg gtgataaaac ttatgcccgt
      121 ctcggcttta aaggagaaac gcaaatcaat gatcaaatga ttggttttgg tcactgggaa
      181 tatgatttta aaggctataa cgatgaagcc aacggctcgc gcggcaacaa tctctgattg
      241 agttaagtgc ccatcacaat atgatcactg accttatatt acacaatcat cccaggatga
      301 aaacaatcac tttaaacgac aaccatattg cacatttaaa cgccaaaaac actacaaaac
      361 tggaatattt aaacttaagc aataacaatt tactgccaac caatgacatt gatcaactaa
      421 tatcatcaaa acatctttgg catgtattag ttaacggcat caacaatgat ccacttgcac
      481 aaatgcagta ctggactgca gtaagaaata taattgatga cactaatgaa gtgaccattg
      541 atttatcagg acttaattta accactcaac caccagggct gcaaaacttc acctctatca
      601 atcttgataa taaccaactc acacatttta atgcaaccaa ctacgataga ctcgtaaagc
      661 taagtctgaa tagtaatact cttgagtcaa taaattttcc tcaaggcaga aatgtaagta
      721 ttacacatat a
//
LOCUS       DAPHDW010000378          719 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.386, whole genome shotgun sequence.
ACCESSION   DAPHDW010000378 DAPHDW010000000
VERSION     DAPHDW010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..719
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.386"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>719
                     /gene="ag43"
                     /locus_tag="RH057_004668"
     CDS             <1..>719
                     /gene="ag43"
                     /locus_tag="RH057_004668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HDV3130867.1"
                     /translation="AINGATTEEGAFVQGNKLQAGAFNYSLNRDSDESWYLRSENAYR
                     AEVPLYASMLTQAMDYDRIVAGSRSHQTGVNGENNSVRLSIQGGHLGHDNNGGIARGA
                     TPESSGSYGFVRLEGDLMRTEVAGMSVTAGIYGAAGHSSVDVKDDDGSRAGTVRDDAG
                     SLGGYLNLIHNASGLWADIVAQGTRHSMKASSDNNDFRVRGWGWLGSLETGLPFSITD
                     NLMLEPQLQYTWQGLSLDDGK"
BASE COUNT          155 a          195 c          223 g          146 t
ORIGIN      
        1 agccattaac ggtgccacca cggaggaagg ggcctttgtc caggggaata agctgcaggc
       61 cggtgccttt aactactccc tcaaccggga cagtgatgag agctggtatc tgcgcagtga
      121 aaatgcttat cgtgcagaag tccccctgta tgcctccatg ctgacacagg caatggacta
      181 tgaccggatt gtggcaggct cccgcagcca tcagaccggt gtaaatggtg aaaacaacag
      241 cgtccgtctc agcattcagg gcggtcatct cggtcacgat aacaatggcg gtattgcccg
      301 tggggccacg ccggaaagca gcggcagcta tggcttcgtc cgtctggagg gtgacctgat
      361 gagaacagag gttgccggta tgtctgtgac cgcagggata tatggtgctg caggccattc
      421 ttccgttgat gttaaggatg atgacggctc ccgtgccggc acggtccggg atgatgccgg
      481 cagcctgggc ggatacctga atctgataca caacgcctcc ggcctgtggg ctgacattgt
      541 ggcccaggga acccgccaca gcatgaaagc ctcatcggac aataacgact tccgcgtccg
      601 gggctggggc tggctgggtt cgctggaaac cggtctgccc ttcagtatca ctgacaacct
      661 gatgctggag ccacaactgc agtatacctg gcagggactt tccctggatg acggtaagg
//
LOCUS       DAPHDW010000379          717 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.387, whole genome shotgun sequence.
ACCESSION   DAPHDW010000379 DAPHDW010000000
VERSION     DAPHDW010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..717
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.387"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..297
                     /locus_tag="RH057_004669"
     CDS             <1..297
                     /locus_tag="RH057_004669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HDV3130868.1"
                     /translation="ASLFCSTELNSKSQSPALGLGHEFAHAQYYLLDKENFMALLSRT
                     DKKYENKEEARVITIIESRAAKTLGECTRGAHSGLPFYRVDGPLQTMKITGTPE"
     gene            309..>717
                     /locus_tag="RH057_004670"
     CDS             309..>717
                     /locus_tag="RH057_004670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005141515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130869.1"
                     /translation="MMKYLMVLLSLFSGSVLGMGRVNELCGIDSVKTIEIINLPSYVT
                     TLVPLSKEGLNEIYRYKVVVNEISDLYAGKIIDLLQMKYFRKEKYNNIRWGVSIISKG
                     NNKCEIYFDAFGECGSVNGINVCFEKNEMIGWIK"
BASE COUNT          234 a           81 c          176 g          226 t
ORIGIN      
        1 gcatctttat tttgttcaac tgaattaaac tcgaaatcac aatcgcctgc attaggtttg
       61 ggacatgagt ttgctcatgc gcagtattac cttcttgata aggaaaattt catggctctt
      121 ttaagccgaa cagataagaa atatgagaat aaagaagagg caagggttat tacaataatt
      181 gaatcgcgtg cggcgaaaac tttaggtgag tgtaccagag gagctcatag tggactgcct
      241 ttttatcggg tggatggacc gttgcagacg atgaaaataa cgggtacgcc agaatagtga
      301 ggttttttat gatgaaatat ttgatggttt tattgtctct attttctggt tcggtactgg
      361 ggatggggcg agtgaatgaa ttgtgtggaa tagatagcgt gaaaacgata gagataatta
      421 atcttccatc gtatgttacc acgttagttc cgcttagcaa ggaaggtctt aatgaaatat
      481 acagatataa agtggtggtg aatgaaatta gcgatcttta tgctggtaag ataattgatt
      541 tattgcaaat gaagtatttc agaaaagaaa aatataacaa tataagatgg ggggttagta
      601 tcatttcgaa aggtaataat aaatgtgaaa tttattttga tgcttttggt gaatgtggaa
      661 gtgtgaatgg cattaacgtg tgttttgaaa aaaatgaaat gataggttgg attaaaa
//
LOCUS       DAPHDW010000380          706 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.388, whole genome shotgun sequence.
ACCESSION   DAPHDW010000380 DAPHDW010000000
VERSION     DAPHDW010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..706
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.388"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            31..663
                     /locus_tag="RH057_004671"
                     /pseudo
     CDS             31..663
                     /locus_tag="RH057_004671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT          244 a          101 c          148 g          213 t
ORIGIN      
        1 cgttaaagca tttacactca ggatgaatat atgggaaaaa ttaaaattgt agtttcagat
       61 cagcagccgt ttatgattga tgggataatt ggatttctcg gacattatcc tgatttatat
      121 gaggttgttg ggggctataa agatctgaag aaatctatag cggagtgtaa caaatctaca
      181 gcctaaatat ttattttagg agaatttgca ggtggaatga tggggtccga actcgtaaaa
      241 tgggttaaat cgcataagat agatgctcat attattacat ttgtagcaaa gatgccgtat
      301 attgattcaa taaaattgct tgaagcaggt gcgaaaggat gcgtatggaa aaccagtcac
      361 ccggcgaaac taaatcgtgc tattgattcg attagtaatg gttacactta ttttgatagt
      421 gtacatatgg attgtgaaaa aatatcttcc aggtattcct ctgataatca acttacaaat
      481 cgtgagtcag aaatattaca actcatagct gatggtaaaa caaacaaaga aatcgctaat
      541 ttcttgcagt taagcagaaa aacagtcgaa actcatagac tcaatatcat gaagaaatta
      601 gatgttcaca gtggtatcga gttgatcaaa acagctttac gtatgggtgt ttgcactata
      661 taaaaaaaag aggctttagg ctcattttgc tgaagcctta cgattg
//
LOCUS       DAPHDW010000381          702 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.389, whole genome shotgun sequence.
ACCESSION   DAPHDW010000381 DAPHDW010000000
VERSION     DAPHDW010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.389"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            69..635
                     /gene="slp"
                     /locus_tag="RH057_004672"
     CDS             69..635
                     /gene="slp"
                     /locus_tag="RH057_004672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HDV3130870.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
BASE COUNT          210 a          146 c          154 g          192 t
ORIGIN      
        1 cagaatcaga tgaaaactat aaagaaatat ctattatggt tttaatattt gttgataagg
       61 atagtaacat gaacatgaca aaaggtgcac tcatcctcag cctttcattt ttgcttgccg
      121 catgtagttc aattccgcaa aatatcaaag gcaataacca acctgatatt caaaaaagtt
      181 ttgttgctgt tcataaccag ccggggttat atgttggtca acaagcgcgc tttggtggga
      241 aggttatcaa cgttatcaat ggcaaaacgg atacgttgtt agaaatcgct gtattaccgt
      301 tggatagcta tgcgaagcct gatattgaag ccaactatca gggccgactg ctcgccagac
      361 aaagcggctt ccttgatcca gtgaactatc gtaatcactt tgttaccatc ctcggtacca
      421 ttcagggtga acaaccaggc tttatcaata aagtcccgta taacttcctg gaagtgaata
      481 tgcagggcat ccaggtgtgg catttgagag aagtggttaa taccacctat aacctgtggg
      541 attacggcta tggtgcattc tggccggaac cgggctgggg tgcgccttac tacaccaatg
      601 cggtgagtca ggtaacacct gagctggtca aataacacca ccgaaagatg cagatgcttt
      661 ctcagcatct gcatcatgta ttacataaaa ttaatataca gt
//
LOCUS       DAPHDW010000382          701 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.390, whole genome shotgun sequence.
ACCESSION   DAPHDW010000382 DAPHDW010000000
VERSION     DAPHDW010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 701)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 701)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..701
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.390"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..620)
                     /locus_tag="RH057_004673"
     CDS             complement(<1..620)
                     /locus_tag="RH057_004673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HDV3130871.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAGKPTDKPNLVCG
                     PVQICWHKFARYWDVELREIPMRPGQLFMDPKRMIEACDENTIGVVP"
BASE COUNT          171 a          176 c          170 g          184 t
ORIGIN      
        1 ggcaccacgc cgatggtgtt ttcgtcacag gcttcaatca tgcgtttcgg gtccataaac
       61 aactgaccgg ggcgcatagg gatctcacgc agctccacat cccagtagcg ggcgaattta
      121 tgccagcaga tttgtaccgg accgcacacc aggtttggtt tatccgttgg tttgcctgca
      181 gcttccatac gcttgcgcca acgccatttc atcgccatcc cgccgagcat acaggcctcg
      241 gaagaaccaa tggtgttggt gccaacggcc tgaccatttt tcggcgcagg cgcatgccac
      301 agatcggcaa ccatatttac gcaacgcagg tcgatggctg cggattgcgg atattcttct
      361 ttgtcgatcc agtttttatt gatcgacaaa tccatcaatt tatggacgtt ttcgtcgtcc
      421 caggtctggc agaaagtggc caggttctga cgagcgttgc catcaagata taattcatca
      481 ttgataatct ggaatgcgac atcatcgcgc atttcgtgca gcggaaatcg ttttgactcc
      541 gcgatagtag aaatggcctt tgcgccaaaa cgtgaatcga gtagttctga gcggaaatcc
      601 gttaacagct tctggtccat ttcgaactcc ttaaatttat ttgaaggcaa taaaaaagta
      661 ggatttatcc gcaatggaag caaggcatta caggcttaat t
//
LOCUS       DAPHDW010000383          696 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.392, whole genome shotgun sequence.
ACCESSION   DAPHDW010000383 DAPHDW010000000
VERSION     DAPHDW010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..696
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.392"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>696
                     /locus_tag="RH057_004674"
     CDS             <1..>696
                     /locus_tag="RH057_004674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter domain-containing protein"
                     /protein_id="HDV3130872.1"
                     /translation="TEEGAFVQGNKLQAGAFNYSLNRDSDESWYLRSENAYRAEVPLY
                     ASMLTQAMDYDRILAGSRSHQTGVSGENNSVRLSIQGGHLGHDNNGGIARGATPESSG
                     SYGFVRLEGDLLRTEVAGMSVTAGVYGAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYL
                     NLTHTSSGLWADIVALGTRHSMKASTDNNDFRARGWGWLGSLETGLPFSITDNLMLEP
                     QLQYTWQGLSLDDG"
BASE COUNT          145 a          191 c          216 g          144 t
ORIGIN      
        1 acggaggaag gggcctttgt ccaggggaat aagctgcagg ccggtgcctt taactactcc
       61 ctcaaccggg acagtgatga gagctggtat ctgcgcagtg aaaatgctta tcgtgcagaa
      121 gtccccctgt atgcctccat gctgacacag gcaatggact atgaccggat tctggcaggc
      181 tcccgcagcc atcagaccgg tgtaagcggt gaaaataaca gcgtccgtct cagcattcag
      241 ggcggtcatc tcggtcacga taacaacggc ggtattgccc gtggagccac gccggaaagc
      301 agcggcagct atggcttcgt ccgtctggag ggtgacctgc tcagaacaga ggttgccggt
      361 atgtctgtga ccgcgggggt atatggtgct gctggccatt cttccgttga tgttaaggat
      421 gatgacggct cccgtgccgg cacggtccgg gatgatgccg gcagcctggg cggatacctg
      481 aatctgacac atacatcctc cggcctgtgg gctgacattg tggccctggg aacccgccac
      541 agcatgaaag cgtcaacgga caataacgac ttccgcgccc ggggttgggg ctggctgggt
      601 tcactggaaa ccggtctgcc cttcagtatc actgacaacc tgatgctgga gccacaactg
      661 cagtatacct ggcagggact ttccctggat gacggt
//
LOCUS       DAPHDW010000384          695 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.393, whole genome shotgun sequence.
ACCESSION   DAPHDW010000384 DAPHDW010000000
VERSION     DAPHDW010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..695
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.393"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>695
                     /locus_tag="RH057_004675"
     CDS             <1..>695
                     /locus_tag="RH057_004675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000132386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HDV3130873.1"
                     /translation="LRFENIFTRHRYIGPEGKALHLRSHQPRHLLNTIGQRNGMSDLD
                     LAKWSGRSLVTQNPVYNHVSEDEMLRKAETLDISTIGSQAFMIDSVKPNLPVTPGMIN
                     LWEHGAVHATEFGYCVHDYMMSPCDKFRDCINCNEQVCVKGEEEKLQRLKERRNMTEG
                     LLSRARESMTEEELGADRWVQHHELTLTRLNELIAILSNNEIPVGSRIKPGGESFTQL
                     GRVLNKFENLENN"
BASE COUNT          208 a          154 c          173 g          160 t
ORIGIN      
        1 gctaaggttt gaaaacatat ttaccagaca tcgctatatt ggcccggaag gaaaagctct
       61 gcatttgaga agccatcagc ctcgtcattt actcaatacg attggacagc gaaacgggat
      121 gtcagatctc gacctagcga agtggtccgg gagatcgctg gttacccaaa acccggttta
      181 caaccacgta tcagaagatg agatgctccg taaggccgag acactggata tcagcactat
      241 cggctcacag gccttcatga ttgattcagt aaagccgaac cttcctgtta ctccgggtat
      301 gatcaatctc tgggaacatg gtgcagtgca tgccaccgag ttcggctact gtgttcatga
      361 ctatatgatg agcccctgcg acaagttcag ggattgtatc aactgcaacg agcaagtttg
      421 tgtgaaaggc gaagaagaga agctccagcg gctcaaggaa aggcgcaata tgacagaagg
      481 gcttctctcc agagccaggg agtcaatgac ggaagaagaa ctgggtgctg accgatgggt
      541 tcagcatcat gaactgaccc ttactcgatt gaatgaattg atcgcaatcc tttcaaataa
      601 cgaaatacca gtcggttcac gtataaaacc cggtggggaa agtttcactc agcttgggcg
      661 agtattaaat aaatttgaaa atctcgaaaa taata
//
LOCUS       DAPHDW010000385          689 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.394, whole genome shotgun sequence.
ACCESSION   DAPHDW010000385 DAPHDW010000000
VERSION     DAPHDW010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..689
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.394"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(390..>689)
                     /locus_tag="RH057_004676"
     CDS             complement(390..>689)
                     /locus_tag="RH057_004676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HDV3130874.1"
                     /translation="CYNLMLSEPGTKWTANKVARYLYISVSTLHRRLASEGVSFQSIL
                     DDVRLNNALSAIQTTVKPISEIARENGYKCPSRFTERFHNRFKITPRELRKASRE"
BASE COUNT          204 a          149 c          120 g          216 t
ORIGIN      
        1 ataatagttc aaataaaagg gctcacgatg aaaaaattaa cagtggcaat ttctgctgta
       61 gctgcatctt aacgtataaa ctagatttaa taacaacgat atccgtacct atccggatat
      121 ctaatgtcat aagcatccct ttaattatcc cctcaacctc ttgtaacttt cccactcttg
      181 aactccacat agtccaaacg ccggatgcaa aaaaatcaca cgtgcatttc tgaaattgtg
      241 atggaggaag atagtatcaa ttatatgtgc ttataaacgt ccccgattag cgcagaagtc
      301 accggattgt tcaggctcca ataggttgag tatgccgtat ttattttcca aaataaaaat
      361 cgatgctgtt gctccttcat aaaatacttt tactctctgg acgcttttct tagctctctt
      421 ggtgttatct taaaacgatt atggaacctt tcagtaaaac gagaaggaca cttgtaacca
      481 ttttccctgg caatctcgct gatgggtttt actgtcgttt gtatagcaga caacgcatta
      541 tttaacctca cgtcgtccaa tatactttga aaacttactc cctcgcttgc cagacggcga
      601 tgcaatgtgg aaacagaaat gtagagatat ctcgctacct tgtttgctgt ccattttgtt
      661 ccaggttcgg atagcattag gttataaca
//
LOCUS       DAPHDW010000386          687 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.395, whole genome shotgun sequence.
ACCESSION   DAPHDW010000386 DAPHDW010000000
VERSION     DAPHDW010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..687
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.395"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..103
                     /locus_tag="RH057_004677"
     CDS             <1..103
                     /locus_tag="RH057_004677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GhoT/OrtT family toxin"
                     /protein_id="HDV3130875.1"
                     /translation="LSHEKTNIRLLSAFLVGITWPISLPVALLFSLF"
     gene            complement(171..>687)
                     /locus_tag="RH057_004678"
     CDS             complement(171..>687)
                     /locus_tag="RH057_004678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014343890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HDV3130876.1"
                     /translation="QMIAVFSGTSGLLFQLFSVRNLPILALIMGLSFALYGLIRKFIH
                     YDAITSVTLETFWSLPVSLILFIYTDPDVTTPSEIPCILYIMTAPVTVIPLVLFAVAL
                     NHTSLIVTGLAQYIEPSMQFLIAVFIFGEVIHYDELFCFSAVWLGLLLCIIESCYRHY
                     RQIPVCKHHTR"
BASE COUNT          237 a          125 c          145 g          180 t
ORIGIN      
        1 tctctcacat gagaaaacta acatccgttt acttagtgcc tttctggtgg ggataacctg
       61 gccaataagt ctgcctgtag cattactctt ttctcttttt taattataat ggtcagcatt
      121 tttatacttt tgaaagtaat aaaaaatacc tgaaatataa cggatgctta ttaccgggta
      181 tggtgtttac agacaggaat ctgacggtaa tgacgataac agctttcgat aatgcacaac
      241 aataacccca accagactgc agaaaagcag aataattcat cataatgtat aacttcaccg
      301 aatataaaca cagcaatcag gaattgcata gaaggttcga tatattgagc aaggccggtt
      361 acgataagtg atgtatgatt cagtgcaaca gcaaacagca ccaacgggat aacagtgact
      421 ggtgcagtca taatgtacag aatacatggg atctcagagg gcgtggttac atccgggtcg
      481 gtatatataa aaaggataag tgaaacaggt aatgaccaga atgtttcaag cgtgactgag
      541 gttatagcat catagtgaat gaattttctt atcagtccgt ataatgcaaa tgaaagcccc
      601 atgattagtg ccagtatggg aaggttgcgt accgaaaaca actgaaatag cagtccagac
      661 gtaccggaaa aaacagcaat catctgg
//
LOCUS       DAPHDW010000387          684 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.396, whole genome shotgun sequence.
ACCESSION   DAPHDW010000387 DAPHDW010000000
VERSION     DAPHDW010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..684
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.396"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            1..247
                     /locus_tag="RH057_004679"
                     /pseudo
     CDS             1..247
                     /locus_tag="RH057_004679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026224.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
BASE COUNT          204 a          123 c          182 g          175 t
ORIGIN      
        1 gtggtgcgca gaatatgatg aatggggcaa cctgctgaat gaagagaacc cgcatcagct
       61 gcagcagctt atccggctgc cggggcagca gtatgatgag gagtccggcc tgtattacaa
      121 ccgccaccgc tattatgacc cgctgcaggg gcgatatatc actcaggatc cgattgggct
      181 gaagggggga tggaattttt atcagtatcc gttgaatccg atctcaaata tagatccatt
      241 aggattagaa acactaaaat gcattaagcc actgcattca atgggcggaa ctggtgaaag
      301 aagcggtcca gatatatggg ggaatccgtt ctatcatcaa tatctttgtg tcccagatgg
      361 taaaggggac tatacttgtg gtggccaaga ccaacgggga gaatcaaaag gagatggtct
      421 atgggggcca ggtaaagcaa gtaatgatac aaaagaagct gctggccgtt gtgacctcgt
      481 tgaaaccgat aatagttgtg tggagaactg tttaaaaggg aagtttaaag aggtaaggcc
      541 gcgttattct gtattgcctg atatattcac acctataaat ttagggctat ttaaaaactg
      601 ccaagactgg tctaatgatt ctttagaaac atgtaagatg aagtgctccg gaaataacat
      661 tggacgtttt attagatttg tatt
//
LOCUS       DAPHDW010000388          683 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.397, whole genome shotgun sequence.
ACCESSION   DAPHDW010000388 DAPHDW010000000
VERSION     DAPHDW010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.397"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..362)
                     /locus_tag="RH057_004680"
     CDS             complement(<1..362)
                     /locus_tag="RH057_004680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130877.1"
                     /translation="MKGFPIAHIFHPSIPPMHAVVNNHNRNIDYWTVKRKFAEIVSTN
                     DVNKIYSISNELRRVLSAITALNFYQGDVPSVMIRIQPENMSPFIIDISTGEHDDYII
                     QTLDVGTFAPFGEQCTCSA"
     gene            complement(618..>683)
                     /locus_tag="RH057_004681"
     CDS             complement(618..>683)
                     /locus_tag="RH057_004681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HDV3130878.1"
                     /translation="LINVNFFSSDRTSFCECNRFP"
BASE COUNT          203 a          134 c          133 g          213 t
ORIGIN      
        1 gctgagcaag tacattgttc gccaaaaggt gcaaaagtgc ctacatctaa tgtttgtatg
       61 atataatcat catgttcccc tgtagaaata tctatgatga atggactcat attttccggt
      121 tggattcgga tcattacaga aggaacatcg ccttgataga aattcaatgc agtaattgca
      181 gataatactc tccgcagttc attacttata ctgtaaattt tattaacgtc attggtggag
      241 acaatttctg caaactttct ttttaccgtc caataatcaa tatttctatt gtgattgtta
      301 accactgcgt gcattggcgg gattgaaggg tgaaaaatat gtgcaattgg gaagcctttc
      361 attgatgcct gccttgtcgt catgacaaac aataatagat gcttattttc ctcgcagggt
      421 tctttcgcac tatcaaaaaa cgctcattta cccggcgtta cagtaaacga agcaacattc
      481 tgtggcctac gacgatcaaa agctctgtga aaatttccct tgttgtcttt gtctgacctt
      541 atcgcggcga ccagcaatta ttcgttttgt gatcctcaac acaaaatgct attcgtcgcc
      601 tatgagggga gaagaattta tggaaaacga ttgcattcac aaaatgacgt cctgtcagaa
      661 gagaaaaagt tgacgttaat caa
//
LOCUS       DAPHDW010000389          683 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.398, whole genome shotgun sequence.
ACCESSION   DAPHDW010000389 DAPHDW010000000
VERSION     DAPHDW010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..683
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.398"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..206)
                     /locus_tag="RH057_004682"
     CDS             complement(<1..206)
                     /locus_tag="RH057_004682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001564245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3130879.1"
                     /translation="MITPVSLMDDQMVDMAFITQLTGLTDKWFYKLIKDGDFPAPIKM
                     GRSSRWLKSEVEAWLQARIAQSRP"
BASE COUNT          189 a          141 c          201 g          152 t
ORIGIN      
        1 tacggacggg actgtgcaat acgcgcctgc agccaggctt ccacttcgct tttcagccat
       61 cgggagctgc ggcccatttt gatgggcgca ggaaagtcac catccttgat gagtttgtaa
      121 aaccacttat cggtcaggcc ggtcagttga gtgataaatg ccatgtcgac catctggtca
      181 tccatcagtg aaactggggt gatcatgaga atgttcctcc ggatgttgtt aaaccagccg
      241 gtgtggtcat gaaaacaacg caccaccttc accggcataa caaccgggga gaaacgtaaa
      301 aaataatcgg gaatgtgacc gccaggagat aaacggggag gttacggtgg ctgagccgta
      361 ccggtatgcc gggtggttgt tacgaaacga tgcttccgca cgcgcgcgcg ataaagaagg
      421 gatgattttc gtgaggaaga ggggaaaact ggcgttctga taattgcaga gttatttacg
      481 gggcggttac gatggggcag accgtgtccg gtgggcaggc gacaggtatc accgatggga
      541 taagactgtc tgattggtat tataaatata taagagagat caaacacatg agatcaaata
      601 catgagttca tatatgtgaa ataaacacgg tgagtgtgat cagtgggcat gatgagaacg
      661 cagtagcaac agactgcgct atc
//
LOCUS       DAPHDW010000390          682 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.399, whole genome shotgun sequence.
ACCESSION   DAPHDW010000390 DAPHDW010000000
VERSION     DAPHDW010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.399"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>682
                     /locus_tag="RH057_004683"
     CDS             <1..>682
                     /locus_tag="RH057_004683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 family protein"
                     /protein_id="HDV3130880.1"
                     /translation="IQRITHNLWHARKKNVFLIEQITNLKVWVNKYIDDCTDEDLNDR
                     DFIASVVDRAIFHFAINSICNPGDNKDATPIERCTFDVETKNGLPSTVQLFYEESKDN
                     EPLANIHFQAIGSGFLTFVNACQEHDDNSLKLFASLLISLSYSSAYTDLAGAEKVNIN
                     EYNENYLTAQFEELSQRDMKKYLGEMKHLADRGGMKFDDYLDKMSLLVNEGKLEPDIL
                     SKMRDAAPK"
BASE COUNT          222 a          107 c          144 g          209 t
ORIGIN      
        1 attcaaagaa tcactcataa tttatggcat gcacgtaaaa aaaatgtgtt tttaattgag
       61 caaataacta atctcaaagt gtgggtcaat aagtatattg acgattgcac tgatgaggat
      121 ttaaacgatc gtgactttat tgcatcagtt gttgaccggg ctatttttca tttcgcgatt
      181 aatagtatat gtaatcctgg ggataataaa gatgcgactc ccattgaacg atgtactttt
      241 gatgtagaaa ctaagaatgg ccttccctcc acggttcagc tattttatga ggaatctaag
      301 gataatgaac ctttagcgaa tatacatttt caagcaatag gttctggttt tttaacgttt
      361 gttaatgcct gccaggaaca tgatgacaac agcttaaaat tatttgcttc gctgttaatt
      421 tcactttcat attctagtgc ctacacagat ttagccggag cagaaaaagt gaatattaat
      481 gaatataatg agaactatct gacagctcag tttgaagaat tatctcaacg tgatatgaag
      541 aagtacctgg gagagatgaa gcatctggcg gaccgggggg ggatgaaatt tgatgactat
      601 ctggataaaa tgtcacttct ggtgaatgaa ggaaagctcg aacctgatat tttaagcaaa
      661 atgcgagatg ctgcaccgaa at
//
LOCUS       DAPHDW010000391          682 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.400, whole genome shotgun sequence.
ACCESSION   DAPHDW010000391 DAPHDW010000000
VERSION     DAPHDW010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..682
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.400"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            33..>682
                     /locus_tag="RH057_004684"
     CDS             33..>682
                     /locus_tag="RH057_004684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130881.1"
                     /translation="MIFSALKEIYDGDPGFIFDKISHKLRHTVTEFDESGNSEPTDLF
                     TWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGDSLTQYCSENARS
                     ASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRAIRYNFQVDDAKF
                     RRDNVKEIISTLFANKVDVDHPENKYKDFKDLEDKVEKRLQNRQTEYQNEINQLSAP"
BASE COUNT          256 a          116 c          137 g          173 t
ORIGIN      
        1 ttgagaagac tcatgatatc aataacaagg aaatgatctt cagcgcatta aaagaaatat
       61 atgatggaga tcctggtttt atcttcgata agatatcaca caaactcaga catacggtaa
      121 cggaatttga tgagagcggg aatagcgaac caacggactt atttacctgg tacggtaaag
      181 ataaaaaagg cgactctctc gctattgtaa ttaaaaataa aaacggaaat gattacttat
      241 ctctcggtta ctacgatcag gacgactacc acattcaaag aggaattcgt attaatggtg
      301 atagtctcac ccaatattgt agtgaaaacg ccaggagtgc ttcagcgtgg tttgaaagca
      361 gcaaagctat catggcagaa tcatttgcaa caggttccga tcatcaggtt gtaaacgagc
      421 tcaacgggga aagactgaga gaacctaacg aagtttttaa acgtttaggt cgagcaataa
      481 gatataattt tcaagtggac gatgcaaaat ttagacgcga taatgtaaaa gaaataattt
      541 ctactttatt cgctaacaaa gttgacgttg accatcctga aaataaatac aaagacttta
      601 aggatctcga agataaagtt gaaaaaaggc ttcaaaatcg ccagacggaa tatcaaaatg
      661 aaattaatca actatctgcg cc
//
LOCUS       DAPHDW010000392          668 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.401, whole genome shotgun sequence.
ACCESSION   DAPHDW010000392 DAPHDW010000000
VERSION     DAPHDW010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.401"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>668
                     /gene="tuf"
                     /locus_tag="RH057_004685"
     CDS             <1..>668
                     /gene="tuf"
                     /locus_tag="RH057_004685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HDV3130882.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILELAGFLDSYIPEPERAIDKPFLLPIEDVFSISGRGTVVTGRVERGII
                     KVGEEVEIVGIKETQKSTCTGVEMFRKLLDEGRAGENVGVLLRGIKREEIERGQVLAK
                     PGTI"
BASE COUNT          154 a          160 c          199 g          155 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aactggctgg cttcctggat
      361 tcttatattc cggaaccaga gcgtgcgatt gacaagccgt tcctgctgcc gatcgaagac
      421 gtattctcca tctccggtcg tggtaccgtt gttaccggtc gtgtagaacg cggtatcatc
      481 aaagttggtg aagaagttga aatcgttggt atcaaagaga ctcagaagtc tacctgtact
      541 ggcgttgaaa tgttccgcaa actgctggac gaaggccgtg ctggtgagaa cgtaggtgtt
      601 ctgctgcgtg gtatcaaacg tgaagaaatc gaacgtggtc aggtactggc taagccgggc
      661 accatcaa
//
LOCUS       DAPHDW010000393          663 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.402, whole genome shotgun sequence.
ACCESSION   DAPHDW010000393 DAPHDW010000000
VERSION     DAPHDW010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.402"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..555)
                     /gene="fimE"
                     /locus_tag="RH057_004686"
     CDS             complement(<1..555)
                     /gene="fimE"
                     /locus_tag="RH057_004686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HDV3130883.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWER"
BASE COUNT          198 a          171 c          136 g          158 t
ORIGIN      
        1 tctttcccat aatccggcaa aacgagcagc attactggcg gtataacgca cagtatggcg
       61 aatatttcga tgcccgagat aatcctgaat taaacgagta tctgcaccac gctccgccag
      121 ttcataaccg caagcatgcc ttaacatatg aggatgagtc tgcgttacgg ttccagcttc
      181 aataccggca tcgcgaataa tgcgataggc ctgctggcga gaaagccgac tcccgcggcg
      241 agaaataaat atagcgtcag tccggtcagc gcctttccag ttagcacgtt cctgggtcca
      301 tcgttccacg gcttcacgct catcaaaacg taacgggtga acggtagaaa atccgttctt
      361 cagtcggcga atatttattc taccttcatt aaggtcaagg tcctgataat gcagatcaag
      421 cagttcacta atacgcatcc catgccgata tgccaacaga ataagacaat aatctctggc
      481 tcccgttgcc ccgtaacaaa ccgcctgcat catggcctga acttctttac cggtaagata
      541 acgacgttta ctcacaatag tagtactcct aactgagata tatttgaatg aatacctata
      601 ggaaacctca atcggtcaaa attagcccaa tagataaaaa gataaccaca cacggagtga
      661 tgt
//
LOCUS       DAPHDW010000394          658 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.403, whole genome shotgun sequence.
ACCESSION   DAPHDW010000394 DAPHDW010000000
VERSION     DAPHDW010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..658
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.403"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          208 a          146 c          118 g          186 t
ORIGIN      
        1 gatttatgaa tttcgttcat aacccctgac aaatcctgcc gtctaatcga gcttttccct
       61 ttacgttatg cttctcatga acgagaacac aacttattca tgaattaacc attccggata
      121 aactatggaa tcaaattcag gcatcgaaag gaactggctg gtttccgtta atagattaaa
      181 tatctattac tctcccgaga gtaaaactat caaatcaatg agtaaccgat acagcctcac
      241 aaattctttc agataattaa cccgtcactg cggcacgttc tctcataagg cgtgcctgca
      301 gtgtaatcca gtatgtatcc ggcatagttg acggtttaat agaactcaat tcctttccca
      361 ggttaccaac aatgatatct tgtataacac cccaggagat acgacattat gatgaaatga
      421 ggtatgctcc tcagcgccaa tcaacgcccg gactgggcca gtcttgagat cagactcatt
      481 cccggaactg acttcaggcg acatatatca atgacgttgg tgcaataaaa gatagccgat
      541 gtaatacatc tattcaaatt tgtgatacat atcaaaaaat tggttgacca aactcgttat
      601 ttatataagg gcacttacga agtgcgctct tttttaaagc gaggaagtac caatgaaa
//
LOCUS       DAPHDW010000395          642 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.404, whole genome shotgun sequence.
ACCESSION   DAPHDW010000395 DAPHDW010000000
VERSION     DAPHDW010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 642)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 642)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..642
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.404"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..370)
                     /locus_tag="RH057_004687"
     CDS             complement(<1..370)
                     /locus_tag="RH057_004687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130884.1"
                     /translation="MIFYLIDKEVKDREMSFNTTHEKSEIYRLILRESELITAWVKSG
                     DTPSAVYGKLRDKNPDIIFSINGFLYNLRNFNYALYETATKNKSKTRLIILNHYDDIA
                     SAIRAGHTLKGVYKLVCPHIT"
BASE COUNT          188 a          113 c          119 g          222 t
ORIGIN      
        1 atgttatatg tgggcatacg agtttgtaaa cacctttaag cgtatgacca gcacggatag
       61 ctgatgcaat atcatcataa tggttgagta ttattaacct tgttttgctt ttgttctttg
      121 tggctgtttc atatagtgca tagttaaagt tacgtaggtt atataaaaag ccatttatcg
      181 aaaagattat atccgggttt ttatcacgta gttttccgta aactgctgac ggcgtgtctc
      241 cagattttac ccatgctgta atgagttcac tttctctaag gatcagacga tagatttcag
      301 atttctcgtg tgtggtatta aaactcatct ctcggtcctt aacttctttg tcaatgagat
      361 aaaaaatcat atttctataa tttagtcaac actctgtgct acatgcgata tggatatacc
      421 agcaatatgc aatgcgtgag aaaatgatgt ttttattgca tcatctatga tgagtgaaaa
      481 caactaaagt tgaaagctac acaaaactgg ccccatagtt atcgggactg aataccataa
      541 accattcata gtcaagcgtg tattcaacca tatcagttaa accggtttga ctggcctgaa
      601 agtatctcca tatccgttgt gataattttg ttctctttag tg
//
LOCUS       DAPHDW010000396          640 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.405, whole genome shotgun sequence.
ACCESSION   DAPHDW010000396 DAPHDW010000000
VERSION     DAPHDW010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..640
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.405"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
BASE COUNT          181 a          146 c          137 g          176 t
ORIGIN      
        1 ccacgttttc agtgggctga ttagtccaaa agctgaagtt cttggtgctg aataaatcct
       61 gaatgaattt tccttgtagt ggtcaataaa aaccgaactc cattttattt tttatttctc
      121 tgcacacctc agtcagtttg atcagctgct gaaaaaactc agcgttgaag ccgctttcaa
      181 tgcagaaatg gcacaccatc tcgggtatga aaaaaatcag ccccgtatta ccgggacgga
      241 taactgcaag tatcccgcat aatcgtgcca ttcatattta gaaatcatcc ggcataatca
      301 atctgccaac gaaggagatc gctatgcgta cagcccgttt tactgagcat cagatcatcg
      361 ctgtgattaa gtcgattgaa gccggacgaa ttgttaaaga tgtttgctga cctgagcctg
      421 gagaaccgag tgctgaagga tgttatcgaa aaagctttaa aacaagcctg taagcgtgag
      481 ttggttactc atctgataac gacattcggg ctcagcatcc gtcaggccta ccagagtttg
      541 aacctgagca gaacggctta ccattaccgc ccagatacca cgcgtgatga acccgttata
      601 aacggggcta tttatatgct taccttttgt gtattaccct
//
LOCUS       DAPHDW010000397          635 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.406, whole genome shotgun sequence.
ACCESSION   DAPHDW010000397 DAPHDW010000000
VERSION     DAPHDW010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.406"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>635
                     /locus_tag="RH057_004688"
     CDS             <1..>635
                     /locus_tag="RH057_004688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS2 family transposase"
                     /protein_id="HDV3130885.1"
                     /translation="MPAINAKRVYRIMRQNALLLERKPAVPPSKRAHTGRVAVKESNQ
                     RWCSDGFEFCCDNGERLRVTFALDCCDREALHWAVTTGGFNSETVQDVMLGAVERRFG
                     NDLPSSPVEWLTDNGSCYRANETRQFARMLGLEPKNTAVRSPESNGIAESFVKTIKRD
                     YISIMPKPDGLTAAKNLAEAFEHYNEWHPHSALGYRSPREYLRQRACNGLS"
BASE COUNT          157 a          152 c          198 g          128 t
ORIGIN      
        1 gtatgcctgc gatcaatgcc aaacgtgttt accggatcat gcgccagaat gcgctgttgc
       61 ttgagcgaaa acctgctgta ccgccatcga aacgggcaca tacaggcaga gtggccgtga
      121 aagaaagcaa tcagcgatgg tgctctgacg ggttcgagtt ctgctgtgat aacggagaga
      181 gactgcgtgt cacgttcgcg ctggactgct gtgatcgtga ggcactgcac tgggcggtca
      241 ctaccggcgg cttcaacagt gaaacagtac aggacgtcat gctgggagcg gtggaacgcc
      301 gcttcggcaa cgatcttccg tcgtctccag tggagtggct gacggataat ggttcatgct
      361 accgggctaa tgaaacacgc cagttcgccc ggatgttggg acttgaaccg aagaacacgg
      421 cggtgcggag tccggagagt aacggaatag cagagagctt cgtgaaaacg ataaagcgtg
      481 actacatcag tatcatgccc aaaccagacg ggttaacggc agcaaagaac cttgcagagg
      541 cgttcgagca ttataacgaa tggcatccgc atagtgcgct gggttatcgc tcgccacggg
      601 aatatctgcg gcagcgggct tgtaatgggt taagt
//
LOCUS       DAPHDW010000398          631 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.407, whole genome shotgun sequence.
ACCESSION   DAPHDW010000398 DAPHDW010000000
VERSION     DAPHDW010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 631)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 631)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..631
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.407"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>631
                     /locus_tag="RH057_004689"
     CDS             <1..>631
                     /locus_tag="RH057_004689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3130886.1"
                     /translation="IESVWKNIIVSDESLTRCIYSLRCIFEKIGYDRCIETIYRKGYR
                     FSGQVFKTKINEDNTSDYSIAIFPFTTSLNTLDPLILNQELVQIISNKKIDGLYTYPM
                     AATNFCNDHISQNSFLSRFKPDYFVTGRINQNNAVNTLYIELIDAKNLFLIASNHLPV
                     DELHNTSQFIIDNILQTVHKPERSVRLAKQDQGYKNHYLSDEMLAGKKEL"
BASE COUNT          225 a          114 c           99 g          193 t
ORIGIN      
        1 atcgaatcag tatggaaaaa tattattgtt agtgacgagt ccctgacaag atgtatctat
       61 tctttgcgct gcatttttga aaaaattggc tatgatcgtt gcatagaaac aatctaccgg
      121 aaaggttatc gtttcagcgg gcaggttttc aaaactaaaa ttaatgaaga taatacttca
      181 gactattcca tagctatatt ccctttcact acttcattga atacactgga tccattaata
      241 cttaatcagg aattagtgca aatcatttca aataaaaaaa tcgatggtct ctatacctat
      301 ccgatggctg cgacaaattt ttgtaatgat cacatatctc aaaattcatt cttgagcaga
      361 ttcaagccag attatttcgt tacaggaaga ataaaccaga ataatgcagt gaacacttta
      421 tacattgagt tgatcgacgc taaaaacctt ttcctcatcg ccagtaatca tctccctgtt
      481 gatgaactac ataatacatc acaatttatt atagataata tccttcaaac ggtacataaa
      541 ccagaacgat ctgtaagatt agctaagcag gaccaaggat ataagaatca ttatttatca
      601 gatgaaatgt tagccggaaa gaaagaactt t
//
LOCUS       DAPHDW010000399          622 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.408, whole genome shotgun sequence.
ACCESSION   DAPHDW010000399 DAPHDW010000000
VERSION     DAPHDW010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..622
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.408"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>622)
                     /locus_tag="RH057_004690"
     CDS             complement(<1..>622)
                     /locus_tag="RH057_004690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308264.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion protein Rhs"
                     /protein_id="HDV3130887.1"
                     /translation="GRSIHFEPLFPGEISYSRSESLWLARGGVAAQHSSQPLSALWQV
                     LPEDVRLSPHVYLATNSLQGPWWILSWPERVPGADEVLPPEPPAYRVLTGVVDGFGRT
                     LAFHRAAEGDVAGAVTGVTDGAGRRFHLVLTTQAQRAEVFRKQRATSLSSPAGPRSAS
                     SSLVFPDTLPAGTGYGTDNGIRLEAVWLTHDPAYPDEQPTAPLARYT"
BASE COUNT          108 a          226 c          193 g           95 t
ORIGIN      
        1 acgtgtagcg cgccagcggc gcagtcggct gttcatccgg gtatgccggg tcgtgtgtca
       61 gccataccgc ctccagccgg ataccgttat ccgtgccgta tcctgtaccg gcgggcagcg
      121 tgtcggggaa aaccagagaa gaggaagcag aacggggacc ggcaggggaa gataaagacg
      181 tggcgcgctg tttacggaat acttccgccc gctgtgcctg tgtggtcagc accaggtgaa
      241 aacggcggcc cgcgccgtcc gtcacccccg tcaccgcgcc cgccacatca ccctccgccg
      301 cccggtgaaa ggccagcgtc cgcccgaagc cgtccaccac gcccgtcagc acccggtatg
      361 cgggcggctc cggcggcagc acctcgtccg cccccggcac ccgctccggc cagctgagta
      421 tccaccacgg cccctgaagg ctgtttgtcg ccaggtacac atgcggactc aggcgaacat
      481 cttccggcag cacctgccag agcgcgctta gcggctgcga actgtgctgc gccgctactc
      541 cgccccgcgc cagccagagt gactcgctgc ggctgtagct tatctcgccg ggaaacagcg
      601 gctcaaagtg aatgctcctg cc
//
LOCUS       DAPHDW010000400          614 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.409, whole genome shotgun sequence.
ACCESSION   DAPHDW010000400 DAPHDW010000000
VERSION     DAPHDW010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..614
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.409"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..245)
                     /locus_tag="RH057_004691"
     CDS             complement(<1..245)
                     /locus_tag="RH057_004691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YmgA"
                     /protein_id="HDV3130888.1"
                     /translation="MKTSDNERIKYEITGQAVLQILRMKINFSLQTLIKQLLVMKSAE
                     EDAFRRDLIDSIIRDFSNSDSGGPNRRTATADNKSMF"
     gene            complement(288..524)
                     /locus_tag="RH057_004692"
     CDS             complement(288..524)
                     /locus_tag="RH057_004692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component-system connector protein YcgZ"
                     /protein_id="HDV3130889.1"
                     /translation="MHQNSVTSDSAGAITRYFAKANLPTQQETLGEIVTEILKDGRNL
                     SRKSLCAKLLCRLEQATGEEEQKHYNALIGLLFE"
BASE COUNT          177 a          129 c          110 g          198 t
ORIGIN      
        1 ttgaacatac ttttattgtc agctgtcgct gttcttcggt ttggccctcc tgaatcacta
       61 ttgctaaaat cacgaattat gctgtcaatt aaatcgcgtc ggaaagcatc ttcctcagcc
      121 gatttcatta cgagtaattg cttaataaga gtctgcaatg aaaaatttat cttcatgcgc
      181 agtatctgga gcaccgcctg gccagtaatt tcatatttta tacgttcatt atcagatgtc
      241 ttcataacga cttcatagct aacctgttaa ataatcaggc tgttgcatta ttcaaaaagc
      301 aacccaatta gtgcattata atgtttctgt tcctcttccc ctgtcgcttg ttccagtcgg
      361 caaagaagtt tggcacaaag cgactttcga ctcagattac gtccatcttt caaaatctca
      421 gtcacaattt ccccgagagt ttcctgctga gtaggcaagt tagcttttgc aaagtaacga
      481 gtgattgctc ccgcagaatc tgaagtcact gaattttgat gcatgctacg cctctgttaa
      541 aaatgttaat caatgtgaag tgaggtaaca atagcaaact tgtacgaaat atgtgtacag
      601 aatgataaat taat
//
LOCUS       DAPHDW010000401          605 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.410, whole genome shotgun sequence.
ACCESSION   DAPHDW010000401 DAPHDW010000000
VERSION     DAPHDW010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.410"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..216
                     /locus_tag="RH057_004693"
     CDS             <1..216
                     /locus_tag="RH057_004693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130890.1"
                     /translation="FSSVYKSLTSNLLQRLNNKQGVLRELNSLVNYIDNNQEKAEEIY
                     ATVRAQYEMKVIEKELIHEVRVRTIRL"
     gene            complement(268..582)
                     /locus_tag="RH057_004694"
     CDS             complement(268..582)
                     /locus_tag="RH057_004694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008322889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-binding protein"
                     /protein_id="HDV3130891.1"
                     /translation="MKIYSILTVILTTCLLTACNSEPSQDDIYNVFKNVVDRSNASMK
                     ALNSSIPEKDLLRIDYVKKVSCTEEANNVYNCIVDASISNMKQTKPVKLVKADGVWKE
                     VQ"
BASE COUNT          207 a          107 c          101 g          190 t
ORIGIN      
        1 tttagctcgg tatacaaaag tctgacaagc aatctactac aacgactaaa taacaaacaa
       61 ggtgttctta gagagctaaa ctcgcttgtt aattacatag ataacaatca ggaaaaagca
      121 gaagaaatct atgctactgt cagagcacaa tatgaaatga aagtaattga gaaagagtta
      181 attcatgaag ttcgcgtgag aaccatcaga ttataggcaa taaaaagccc ctatgcaggg
      241 gctaaaagag acttctcgat attactacta ctgaacttct ttccagacac catcagcttt
      301 cacaagctta acaggctttg tctgtttcat attgctaatc gaggcatcga caatgcagtt
      361 ataaacattg tttgcttctt ctgtgcaact tactttcttc acataatcga ttcttaaaag
      421 atctttttcg ggaatacttg agttcagagc tttcatactt gcatttgacc tgtctacaac
      481 atttttaaaa acattgtata tatcgtcttg cgatggttca ctgttacatg cagttagaag
      541 acatgtagta aggataactg taaggattga gtatattttc attttattat ccaaaaataa
      601 ttaat
//
LOCUS       DAPHDW010000402          590 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.411, whole genome shotgun sequence.
ACCESSION   DAPHDW010000402 DAPHDW010000000
VERSION     DAPHDW010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.411"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>590
                     /locus_tag="RH057_004695"
     CDS             <1..>590
                     /locus_tag="RH057_004695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HDV3130892.1"
                     /translation="KAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGG
                     TLAGTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGSLTGNGSVEKSGSGTLTVSN
                     TTLTQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGAT
                     WNIPDNATVLSVVDDLSHAGQIHFTSTRTGKFVPATL"
BASE COUNT          144 a          186 c          161 g           99 t
ORIGIN      
        1 aaggcattca gtatcggagg cggtcaggcg gatgccctga tgctggaaaa aggcagttca
       61 ttcacgctga acgccggtga tacggccacg gataccacgg taaatggcgg actgttcacc
      121 gccaggggcg gcacactggc gggcaccacc acgctgaata acggcgccat acttaccctt
      181 tccgggaaga cggtgaacaa cgataccctg accatccgtg aaggcgatgc actcctgcag
      241 ggaggctctc tcaccggtaa cggcagcgtg gaaaaatcag gaagtggcac actcactgtc
      301 agcaacacca cactcaccca gaaagccgtc aacctgaatg aaggcacgct gacgctgaac
      361 gacagtaccg tcaccacgga tgtcattgct cagcgcggta cagccctgaa gctgaccggc
      421 agcactgtgc tgaacggtgc cattgacccc acgaatgtca ctctcgcctc cggtgccacc
      481 tggaatatcc ccgataacgc cacggtgctg tcggtggtgg atgacctcag ccatgccgga
      541 cagattcatt tcacctccac ccgcacaggg aagttcgtac cggcaaccct
//
LOCUS       DAPHDW010000403          587 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.413, whole genome shotgun sequence.
ACCESSION   DAPHDW010000403 DAPHDW010000000
VERSION     DAPHDW010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.413"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..526)
                     /locus_tag="RH057_004696"
     CDS             complement(<1..526)
                     /locus_tag="RH057_004696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HDV3130893.1"
                     /translation="MANKTEKPTQKKLQDASKKGQILKSRDLTISVIMLVGTLYLGYV
                     FDVHHIMSILEYILDHNAKPDIWDYFKAMGVGWLKTNIPFLLVCMFTTILVSWFQSKM
                     QLATEAVKFKFDSLNPVNGLKRIFGLKTVKEFVKAILYIVFFALAIKVFWSNHKSLLF
                     KTLDGDIISLLSDWG"
BASE COUNT          203 a          104 c           90 g          190 t
ORIGIN      
        1 ctccccaatc tgataataaa gatatgatat ctccatcaag agttttaaaa agcagtgatt
       61 tatgattact ccaaaatact ttgatcgcca atgcaaaaaa aacaatataa agaattgctt
      121 taacaaattc ttttacggtt tttaagccaa atatacgctt taagccattt actggattca
      181 atgaatcaaa cttaaattta acagcttcag ttgctaattg cattttactt tgaaaccagg
      241 agacaagtat tgttgtgaac atgcaaacca gcaaaaaagg aatgttcgtt ttcaaccaac
      301 caacccccat agctttaaaa tagtcccaaa tatctggttt agcgttatga tcaaggatgt
      361 attcaagaat cgacataatg tgatgcacat caaaaacata tccgagatat aaagtaccca
      421 caagcattat tacagagatg gttaagtctc tactttttag aatttgccct tttttagaag
      481 catcctggag tttcttttgt gttggctttt cagttttatt ggccatgaaa cactctattt
      541 ctcaatgtat agttgcaatt gttctgggaa aaaggataac ggcatta
//
LOCUS       DAPHDW010000404          581 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.414, whole genome shotgun sequence.
ACCESSION   DAPHDW010000404 DAPHDW010000000
VERSION     DAPHDW010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..581
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.414"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>581
                     /locus_tag="RH057_004697"
     rRNA            <1..>581
                     /locus_tag="RH057_004697"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          164 a          127 c          177 g          113 t
ORIGIN      
        1 ataatgggtc agcgacttat attctgtagc aaggttaacc gaatagggga gccgaaggga
       61 aaccgagtct taactgggcg ttaagttgca gggtatagac ccgaaacccg gtgatctagc
      121 catgggcagg ttgaaggttg ggtaacacta actggaggac cgaaccgact aatgttgaaa
      181 aattagcgga tgacttgtgg ctgggggtga aaggccaatc aaaccgggag atagctggtt
      241 ctccccgaaa gctatttagg tagcgcctcg tgaattcatc tccgggggta gagcactgtt
      301 tcggcaaggg ggtcatcccg acttaccaac ccgatgcaaa ctgcgaatac cggagaatgt
      361 tatcacggga gacacacggc gggtgctaac gtccgtcgtg aagagggaaa caacccagac
      421 cgccagctaa ggtcccaaag tcatggttaa gtgggaaacg atgtgggaag gcccagacag
      481 ccaggatgtt ggcttagaag cagccatcat ttaaagaaag cgtaatagct cactggtcga
      541 gtcggcctgc gcggaagatg taacggggct aaaccatgca c
//
LOCUS       DAPHDW010000405          573 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.416, whole genome shotgun sequence.
ACCESSION   DAPHDW010000405 DAPHDW010000000
VERSION     DAPHDW010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.416"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>573
                     /locus_tag="RH057_004698"
     CDS             <1..>573
                     /locus_tag="RH057_004698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HDV3130894.1"
                     /translation="KGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIG
                     RFCSIASNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPII
                     GNDVWIGNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVID
                     ELLRIKWWNYNYSDLPDNNKCDDINYFVEEM"
BASE COUNT          185 a           81 c          121 g          186 t
ORIGIN      
        1 aaaggtgaca agataacaat tgaagataat gttattgctg aagaatattc aacaatgcct
       61 gtgaaaaatt tctccagtgt cggcgctttt tcatttccga cctgccattt ttcagggaat
      121 atacgtatag gtcgattttg tagtattgca tcaaatgtaa aaataatggg tggaaatcat
      181 cctttgaatc gttttactac tcacatgatg acttataacg gtgaatttga taagtttgct
      241 atgagtgaat ttgaacgtag ctggacattg aaaccattca ttaccaaacc tgaaaatccg
      301 attataggaa atgatgtctg gataggaaat gatgttgtat tgaaaggtgg catcgcaata
      361 ggtgatggtg ctgttattgc tgccaattcc gttgttacca aagatgtgcc cccatatgcc
      421 atagttgcag gagttcctgc aaaaataatc agatttaggt ttgattcaaa tgttattgat
      481 gaactgttga gaataaaatg gtggaattat aactactcag atcttccgga taataacaag
      541 tgtgatgata taaattattt tgttgaagag atg
//
LOCUS       DAPHDW010000406          572 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.417, whole genome shotgun sequence.
ACCESSION   DAPHDW010000406 DAPHDW010000000
VERSION     DAPHDW010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 572)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 572)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..572
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.417"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..536
                     /locus_tag="RH057_004699"
     CDS             <1..536
                     /locus_tag="RH057_004699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial-like protein"
                     /protein_id="HDV3130895.1"
                     /translation="SLLVAGVAMILSNNVFADEGHGIVKFKGEVISAPCSIKPGDEDL
                     TVNLGEVADTVLKSDQKSLAEPFTIHLQDCMLSQGGTTYSKAKVTFTTANTMTGQTDL
                     LKNTKETEIGGATGVGVRILDSQSGEVTLGTPVVITFNNTNSYQELNFKARMESPSKD
                     ATPGNVYAQADYKIAYE"
BASE COUNT          169 a          129 c          130 g          144 t
ORIGIN      
        1 agtcacttct tgttgctggc gtagcaatga ttctttccaa taacgtattt gctgacgaag
       61 gtcacggcat tgttaaattt aaaggtgaag ttatctctgc tccatgttca attaaaccag
      121 gtgacgaaga cttaactgta aatctgggtg aagtagcaga taccgttctg aaaagcgacc
      181 agaaatcttt ggctgagcct ttcactattc acctgcaaga ctgcatgttg agccagggtg
      241 gaaccactta ttcaaaagcg aaggttactt tcaccacagc gaatactatg acgggtcaga
      301 ctgacctgct gaaaaacacc aaagaaactg aaatcggtgg tgccacaggc gttggcgtac
      361 gtattctgga tagccagagc ggcgaagtga ctttaggtac tccggtagta attacattca
      421 acaatactaa cagctatcag gaactgaact tcaaagctcg tatggaatcc ccaagcaaag
      481 atgctacccc gggcaacgtt tacgctcagg ctgactataa gattgcttac gagtaatcac
      541 attaggccgg gttcgcccgg ccacatcatt aa
//
LOCUS       DAPHDW010000407          570 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.418, whole genome shotgun sequence.
ACCESSION   DAPHDW010000407 DAPHDW010000000
VERSION     DAPHDW010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.418"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..557)
                     /locus_tag="RH057_004700"
     CDS             complement(<1..557)
                     /locus_tag="RH057_004700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001405821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HDV3130896.1"
                     /translation="MSFGQPCDEFPLSALPPLIRDAVIEAQQITQAPLGLVAASALGA
                     VSLVCQNLIDVCRLNTLRGPVSLFFLTLAESGERKTAVDKLLMKPLYQQEMQLYSRYK
                     SELAVWKNKEELLKAQKKALLSKLNKELRKGADESETLRQLEVLQKNSAEEPVRYKFI
                     FNDATTAAIKNQLCGKWRSVGIMSD"
BASE COUNT          148 a          143 c          122 g          157 t
ORIGIN      
        1 tcatctgaca taatacccac tgaacgccat ttaccacaaa gctgattctt aattgccgca
       61 gtcgtggcat cattaaaaat gaatttatac ctcacaggtt cttccgcact gtttttctga
      121 agtacttcca gttgtctgag ggtttctgac tcatccgcgc ctttgcggag ttctttattc
      181 agttttgaca acaatgcttt tttctgagcc tttaaaagct cttccttgtt tttccagaca
      241 gccagttcac ttttgtatct ggaatataac tgcatctcct gctggtacag gggcttcatt
      301 aataatttgt ccactgcggt ttttcgttct ccggactcgg caagcgtcag gaaaaaaaga
      361 gagaccggcc cccgcagtgt attcagtctg cagacatcaa tcagattctg gcagacaagt
      421 gaaacagcgc ccagggcaga cgctgccaca agcccaaggg gagcctgcgt aatttgctga
      481 gcttcaataa cggcatcacg aatgagtgga ggcagcgcac tcaggggaaa ctcatcacaa
      541 ggctgaccga atgacatagt aacatcacta
//
LOCUS       DAPHDW010000408          566 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.419, whole genome shotgun sequence.
ACCESSION   DAPHDW010000408 DAPHDW010000000
VERSION     DAPHDW010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..566
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.419"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            30..497
                     /locus_tag="RH057_004701"
     CDS             30..497
                     /locus_tag="RH057_004701"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130897.1"
                     /translation="MAKKERIYNNKKLEEALKRVTYSGLTSEATLIINQTIQECKSLG
                     KYKLPVKDVFYIVKHATELSKVQVKYYLTLTRSDQMKKFAASESSIEKYKLACTAVAK
                     ALDKFVEEGGTLCGLKKALPGKPLTDEQKNAVQELLNSNASAKDLIAYLRTIV"
BASE COUNT          214 a           97 c          105 g          150 t
ORIGIN      
        1 aattaacatc ttccataggg gtatttatca tggcaaaaaa agaaaggatt tataacaata
       61 aaaagttaga agaagcacta aaacgtgtta cttacagtgg cctaacatca gaagcaactc
      121 taataattaa ccagacgatt caagagtgta aatcattggg aaagtacaag ttacctgtta
      181 aagatgtctt ctacatcgtc aaacacgcta cagagctttc aaaagtacag gttaagtatt
      241 atcttacctt aacccgttca gatcagatga agaaatttgc agcaagtgaa tcatctatcg
      301 agaagtacaa attagcctgt accgcagttg ctaaagcatt ggataagttt gtagaagaag
      361 gtggtacgct atgtggacta aagaaagcat tgcctggtaa gccattaacc gacgaacaga
      421 agaacgcagt acaggaacta ttgaacagta atgcctcagc gaaagatctg attgcatatc
      481 tacgaaccat tgtttaatcg tacctataac cgtaacttaa aactaagaaa tacggttatc
      541 atgattatta gtgaattatt attcaa
//
LOCUS       DAPHDW010000409          549 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.420, whole genome shotgun sequence.
ACCESSION   DAPHDW010000409 DAPHDW010000000
VERSION     DAPHDW010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.420"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            175..250
                     /locus_tag="RH057_004702"
     tRNA            175..250
                     /locus_tag="RH057_004702"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Ile,seq:cat)
BASE COUNT          154 a           96 c          132 g          167 t
ORIGIN      
        1 taacatttgc ttgttgactg cgagtatcga ctttgcgaat ggaagcggtg tcgggtttta
       61 tgtcgcgtgt aatgacgagc aaggatgaat gagaacgcat aaaaatctgt tatttacatc
      121 atatgatcat catgtttatt gcgtggttta taagtattgt tattgcacac tcaaggccct
      181 ttagctcagt ggttagagca ggcgactcat aatcgcttgg tcgctggttc aagtccagca
      241 agggccacca aatcattatt tacgtatcag gaagagttaa gggtacaagg ggtactcgat
      301 ggcggtctat atttcatgac taacgccatt tcaaggaaga agtgcggtgg gtggtttcga
      361 atgagggctg gtgttttggt acgtggcacg ccactgcaat gtatttatcc atcagtactc
      421 attcaaaagt acctggaaaa aatgcctgct atgccaagcg ggcaaattgc ttacatgtgg
      481 aattaagtaa ttaacgataa agccagagta ttatcattta ttatacccct taatttaatc
      541 aggttaatt
//
LOCUS       DAPHDW010000410          548 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.421, whole genome shotgun sequence.
ACCESSION   DAPHDW010000410 DAPHDW010000000
VERSION     DAPHDW010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.421"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>548)
                     /locus_tag="RH057_004703"
     CDS             complement(<1..>548)
                     /locus_tag="RH057_004703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000019445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5-like element IS5 family transposase"
                     /protein_id="HDV3130898.1"
                     /translation="RPYPLETMLRIHCMQHWYNLSDGAMEDALYEIASMRLFARLSLD
                     SALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIEAPSSTK
                     NKEQQRDPEMHQTKKGNQWHFGMKAHIGVDAKSGLTHSLVTTAANEHDLNQLGNLLHG
                     EEQFVSADAGYQGAPQREELAE"
BASE COUNT          100 a          146 c          168 g          134 t
ORIGIN      
        1 ctcggccagc tcctcgcgct gtggcgctcc ttggtagccg gcatcggctg agacaaattg
       61 ctcctctcca tgaagcagat tacccagctg attgaggtca tgctcgttgg ccgcggtggt
      121 gactaggctg tgggtcaggc cactcttggc atcgacacca atgtgggcct tcatgccaaa
      181 gtgccactga ttgcctttct tggtctgatg catctccgga tcgcgttgct gctctttgtt
      241 cttggtagag ctgggtgcct caatgatggt ggcatccacc aaagtgcctt gggtcatcat
      301 gacgcctgct tcggccagcc agcgattgat ggtcttgaac aattgacggg ccagttgatg
      361 ctgctcgagc aggtggcgga aattcatgat ggtggtgcga tccggcaggg cgctatccag
      421 ggataatcgg gcaaacaggc gcatggaggc gatttcgtac agggcatctt ccatggcacc
      481 gtcgctcagg ttgtaccaat gctgcatgca gtgaatacgc agcatggtct ccagcggata
      541 gggccgtc
//
LOCUS       DAPHDW010000411          544 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.422, whole genome shotgun sequence.
ACCESSION   DAPHDW010000411 DAPHDW010000000
VERSION     DAPHDW010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..544
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.422"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..489
                     /locus_tag="RH057_004704"
     CDS             <1..489
                     /locus_tag="RH057_004704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031587700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130899.1"
                     /translation="ETTELYTKLVKWEKDRRFVVSRVLKPEKERAQLSLLEGSEYDYF
                     FFVTNTTLLSEKVVIYYEKRGNAENYIKEAKYDMAVGHLLLKSFWANEAVFQMMMLSY
                     NLFLLFKFDSLDSSEYRQQIKTFRLKYVFLAAKIIKTARYVIMKLSENYPYKGVYEKC
                     LV"
BASE COUNT          194 a           83 c          104 g          163 t
ORIGIN      
        1 gaaactacag aactgtatac aaaattagtt aaatgggaaa aagacagaag atttgtcgta
       61 tctcgcgtac tgaaaccaga aaaagaaaga gcacaattat cacttttaga aggttccgaa
      121 tacgactact ttttctttgt aacaaatact accttgcttt ctgaaaaagt agttatatac
      181 tatgaaaagc gtggtaatgc tgaaaactat atcaaagaag ccaaatacga catggcggtg
      241 ggtcatctct tgctaaagtc attttgggcg aatgaagccg tgtttcaaat gatgatgctt
      301 tcatataacc tatttttgtt gttcaagttt gattccttgg actcttcaga atacagacag
      361 caaataaaga cctttcgttt gaagtatgta tttcttgcag caaaaataat caaaaccgca
      421 agatatgtaa tcatgaagtt gtcggaaaac tatccgtaca agggagtgta tgaaaaatgt
      481 ctggtataat aagaatatca tcaataaaat tgagtgttgc tctgtggata acttgcagag
      541 ttta
//
LOCUS       DAPHDW010000412          532 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.423, whole genome shotgun sequence.
ACCESSION   DAPHDW010000412 DAPHDW010000000
VERSION     DAPHDW010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.423"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..453
                     /locus_tag="RH057_004705"
     CDS             <1..453
                     /locus_tag="RH057_004705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF943 family protein"
                     /protein_id="HDV3130900.1"
                     /translation="LLCCIYNLWTLRPVQILYTYSDAGNSVFLVVDHLPWTDSDKINW
                     YLKHQNEIKNQHPLPEGSWHTWYVIDIGNGFTDYKKYIEGPYEDLYCFPTIKSNDNCI
                     VKNYLMVINEYPYRNTHIGINDFTEYQLTQENKIERVFNPHDFKKDNF"
BASE COUNT          182 a           96 c           91 g          163 t
ORIGIN      
        1 cttctatgct gcatctataa tttatggaca ttgcgtcctg tacagatttt gtatacatac
       61 tctgatgctg gcaattcagt ctttctggtc gttgatcatc tgccatggac tgacagcgat
      121 aaaataaatt ggtatctgaa gcatcagaat gaaattaaaa atcaacatcc cctaccagaa
      181 ggtagttggc atacctggta tgtcattgat atcggtaatg gcttcactga ctataaaaaa
      241 tatatcgaag ggccgtatga agatctctac tgtttcccga caattaagag caatgataat
      301 tgcatcgtga aaaattactt aatggttatt aatgaatacc cttatcgtaa cactcatatt
      361 ggtattaacg attttactga gtatcaatta acacaagaaa ataaaataga gcgagttttc
      421 aatccgcatg actttaaaaa agacaatttt tagaaataag ccggataaaa aaatagcttt
      481 atccggcact catattacaa ccagttcttc cgcttaaagt acagatacgg tg
//
LOCUS       DAPHDW010000413          522 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.424, whole genome shotgun sequence.
ACCESSION   DAPHDW010000413 DAPHDW010000000
VERSION     DAPHDW010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..522
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.424"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            32..>522
                     /locus_tag="RH057_004706"
     CDS             32..>522
                     /locus_tag="RH057_004706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HDV3130901.1"
                     /translation="MFVIWSHGTGFIMSHQLTFADSEFSSKRRQTRKEIFLSRMEQIL
                     PWQNMVEVIEPFYPKAGNGRRPYPLKTMLRIHCMQHWYNLSDGAMEDALYEIASMRLF
                     ARLSLDSALPDRTTIMNFRHLLEQHQLARQLFKTINRWLAEAGVMMTQGTLVDATIIE
                     APSS"
BASE COUNT          127 a          148 c          131 g          116 t
ORIGIN      
        1 gaaggtgcga acaagtccct gatatgagat catgtttgtc atctggagcc atggaacagg
       61 gttcatcatg agtcatcaac ttaccttcgc cgacagtgaa ttcagcagta agcgccgtca
      121 gaccagaaaa gagattttct tgtcccgcat ggagcagatt ctgccatggc aaaacatggt
      181 ggaagtcatc gagccgtttt accccaaggc tggtaatggc cggcgacctt atccgctgaa
      241 aaccatgcta cgcattcact gcatgcagca ttggtacaac ctgagcgatg gcgcgatgga
      301 agatgctctg tacgaaatcg cctccatgcg tctgtttgcc cggttatccc tggatagcgc
      361 cttgccggac cgcaccacca tcatgaattt ccgccacctg ctggagcagc atcaactggc
      421 ccgccaattg ttcaagacca tcaatcgctg gctggccgaa gcaggcgtca tgatgactca
      481 aggcaccttg gtcgatgcca ccatcattga ggcacccagc tc
//
LOCUS       DAPHDW010000414          517 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.425, whole genome shotgun sequence.
ACCESSION   DAPHDW010000414 DAPHDW010000000
VERSION     DAPHDW010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.425"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..359)
                     /locus_tag="RH057_004707"
     CDS             complement(<1..359)
                     /locus_tag="RH057_004707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005460020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-NS histone family protein"
                     /protein_id="HDV3130902.1"
                     /translation="MSESIKILNNIRTLRAQAREFSLETLDEMLEKLTTVVEERRQEY
                     VSYRKEREERQAKLEAFRLQLLEDGIDPADLLSGLSSSSKPKSNREPRPAKYKYIDEN
                     GEQKTWTGQGRTPKAIAT"
BASE COUNT          136 a          113 c          104 g          164 t
ORIGIN      
        1 gtggcaatag ctttaggagt gcgaccttga ccagtccaag tcttctgttc accgttttca
       61 tcgatgtatt tgtacttagc cggtcgtggt tcacggttgg attttggttt agaggacgaa
      121 ctcaaaccag agagcagatc tgccgggtca atgccatcct caaggagttg caggcggaaa
      181 gcttcaagct tggcctgacg ctcttccctc tccttgcgat aggaaacgta ttcctgccga
      241 cgctcttcta ccacagtagt cagcttctca agcatttcgt caagagtttc aagagaaaac
      301 tcacgcgcct gagcacgaag cgtacgaatg ttgtttagta tttttataga ttcgctcata
      361 tgtcttcctt cctggtgatg tttagagcat tgctttatta atgattttct ttatcaaatg
      421 caagagttta cattaaaaac acagaacaac aaacatttag ttacaataat cttcgttaat
      481 cttattcatt aagttttgac aactccaaaa ttgactt
//
LOCUS       DAPHDW010000415          512 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.426, whole genome shotgun sequence.
ACCESSION   DAPHDW010000415 DAPHDW010000000
VERSION     DAPHDW010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..512
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.426"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            58..>512
                     /locus_tag="RH057_004708"
     CDS             58..>512
                     /locus_tag="RH057_004708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010349363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HDV3130903.1"
                     /translation="MSLALHGKSELELAAQKALELLDYVSDITTVDGKILAIMGLITG
                     LSGQAKVSHILFEQAKIHSTDIASLYYYRALVHFHNEKIEEARICIDKSLQLEPRRRK
                     AVVIKECVDMYVPNPLKNNIKLYYKETESESHRVIIDNILKLKQLTRIC"
BASE COUNT          191 a           89 c           93 g          139 t
ORIGIN      
        1 aaaagtgata tacagacgct aaaaacagaa tgttattgcc ttctagcgga atgccatatg
       61 tctttggcac ttcatggaaa aagtgaactt gaacttgctg ctcaaaaagc attagagctt
      121 ttagattatg tatcagacat aaccactgtc gatggaaaaa ttttagctat tatgggactg
      181 ataactggtc tgtctggaca agcaaaagta tctcatatct tatttgaaca ggctaagata
      241 cactcaactg atatagcctc tctctactac tatagggcac tagtccactt tcataatgaa
      301 aaaattgaag aggcaaggat ttgtatagac aaatcactac aactcgaacc cagaagacga
      361 aaagcagttg tgataaaaga atgtgtagat atgtatgtac ctaacccgct caaaaacaac
      421 attaaactct actataaaga aactgagagt gaaagccatc gagttataat tgacaacatt
      481 ttgaaattaa agcagctgac gagaatttgt at
//
LOCUS       DAPHDW010000416          511 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.427, whole genome shotgun sequence.
ACCESSION   DAPHDW010000416 DAPHDW010000000
VERSION     DAPHDW010000416.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.427"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>511
                     /locus_tag="RH057_004709"
     CDS             <1..>511
                     /locus_tag="RH057_004709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
                     /protein_id="HDV3130904.1"
                     /translation="MKELGLISCQQPTHRYKRGGHEHVAIPNYLERQFAVTEPNQVWC
                     GDVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALEMAWETRGKPGGVM
                     FHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRSLKNEWIPVVGYVS
                     FSEAAHAIT"
BASE COUNT          122 a          125 c          160 g          104 t
ORIGIN      
        1 tcatgaaaga gctggggctg atcagctgtc agcagccgac tcaccggtat aaacgtggtg
       61 gtcatgaaca tgttgctatc cctaactacc ttgaacggca gttcgccgtg accgagccaa
      121 atcaggtgtg gtgcggtgat gtgacctata tctggacggg taagcgctgg gcgtacctcg
      181 ccgttgttct cgacctgttc gcaagaaaac cagtgggctg ggccatgtcg ttctcgccgg
      241 acagcaggct caccatgaaa gcgctggaaa tggcatggga aacccgtggt aagcccggcg
      301 gggtgatgtt ccacagcgat cagggcagtc attatacgag caggcagttc cggcagttat
      361 tgtggcgata ccagatcagg cagagtatga gccggcgcgg aaactgctgg gataacagcc
      421 caatggaacg cttcttcaga agtctgaaga acgaatggat accggtggtg ggttacgtaa
      481 gcttcagcga ggcagctcac gccataacgg a
//
LOCUS       DAPHDW010000417          498 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.428, whole genome shotgun sequence.
ACCESSION   DAPHDW010000417 DAPHDW010000000
VERSION     DAPHDW010000417.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.428"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(<1..>498)
                     /locus_tag="RH057_004710"
     CDS             complement(<1..>498)
                     /locus_tag="RH057_004710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SopA family protein"
                     /protein_id="HDV3130905.1"
                     /translation="SDLDLTSCHFKGDVISKVSFLSSNLQHVTFECKEIGDCNFTTAI
                     VDNVIFRCRRLHNVIFIKASGECVDFSKNILDTVDFSQSQLTQSNFREYQIRNSNFDN
                     CYLYASHFTRAEFLSAKEISFIKSNMTAVMFDHVRISTGNFKDCITEQLELTIDYSDI
                     FGNEDL"
BASE COUNT          175 a           91 c           71 g          161 t
ORIGIN      
        1 agatcttcat tcccaaatat atctgaataa tcaatagtta attccaattg ttctgtaatg
       61 caatctttaa aattccctgt cgatattcgc acatgatcaa acataacagc tgtcatattc
      121 gatttaataa atgatatttc tttggcagac agaaactctg ctctggtgaa gtgcgaagcg
      181 taaagataac aattatcgaa gtttgaattt ctaatctgat attcgcgaaa attactctga
      241 gtaagttgac tctgcgagaa gtcaactgta tcaagaatat ttttgctgaa atcgacacat
      301 tcaccactcg ctttgataaa aatcacattg tgtaaacgtc gacatctaaa tatgacatta
      361 tcaactattg cagtagtaaa attgcaatcc ccaatttctt tacattcgaa tgttacatgt
      421 tgtagatttg atgataaaaa agacaccttc gatataacgt cacctttaaa atgacaactt
      481 gttaaatcca aatcagac
//
LOCUS       DAPHDW010000418          498 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.429, whole genome shotgun sequence.
ACCESSION   DAPHDW010000418 DAPHDW010000000
VERSION     DAPHDW010000418.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..498
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.429"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..>498
                     /locus_tag="RH057_004711"
     CDS             <1..>498
                     /locus_tag="RH057_004711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HDV3130906.1"
                     /translation="LERAYKNFFRKRAAFPRFKKRGQNDAFRYPQGVKLDQENSRIFL
                     PKLGWMRYRNSRQVTGVVKNVTVSQSCGKWYISIQTESEVSTPVHPSASMIGLDAGVA
                     KLATLSDGTVFGPVNSFQKNQKTLARLQRQLSRKVKFSNNWQKQKRKIQRLHSRIANI
                     RRDYL"
BASE COUNT          139 a          126 c          129 g          104 t
ORIGIN      
        1 ccttgagcgg gcttacaaaa acttcttccg gaagcgggct gcttttcccc gattcaaaaa
       61 gcggggacag aatgatgcat tccgctaccc gcagggcgta aagctcgatc aggaaaacag
      121 ccgtattttt ctgccgaaac tgggctggat gcgctaccgg aatagccgtc aggtcacggg
      181 tgttgtgaaa aatgtcacgg tcagccagtc ctgcggtaag tggtacatca gtattcagac
      241 agaaagtgaa gtatcaactc cggttcaccc ttcagcatca atgatcgggc tggatgctgg
      301 cgtggctaaa ctcgccacgc tgtcagatgg cacagtcttt gggcctgtaa acagttttca
      361 gaaaaaccag aagacgctgg cgagacttca gcgacagtta agccgcaagg tcaaattcag
      421 caataactgg cagaagcaga aacgcaaaat acagcgactg cattcccgta tcgcaaatat
      481 ccgcagggac taccttca
//
LOCUS       DAPHDW010000419          479 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.denovo.430, whole genome shotgun sequence.
ACCESSION   DAPHDW010000419 DAPHDW010000000
VERSION     DAPHDW010000419.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.denovo.430"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            60..416
                     /locus_tag="RH057_004712"
     CDS             60..416
                     /locus_tag="RH057_004712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001579890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130907.1"
                     /translation="MKRALSVLLSIVPLTTIAATIPDGKTEKPFVIDGEVICLKEIQK
                     QIPPYLKSFEEESGVKYKYNVKSGLSYKDTVGLRLQINRVDSGLDMRVADLDYYWLQE
                     RGSLCQPTYMVVDRSQ"
BASE COUNT          150 a           86 c          114 g          129 t
ORIGIN      
        1 taatgcaacc attacacaaa tttgttctaa acaatgatta tattcaaggg gaagaacaga
       61 tgaaaagagc attatcagta ctgttgtcga tagttccatt aaccacaatt gcggctacta
      121 ttcctgacgg taaaactgaa aagccgttcg taattgatgg ggaagttatt tgtttgaaag
      181 aaattcagaa gcaaatcccc ccttatctca aatcttttga agaagaaagc ggggttaagt
      241 acaagtacaa tgttaaatct ggtttatcgt acaaagatac tgtagggctg cggttacaga
      301 tcaatcgtgt ggattcgggg ttggatatgc gtgttgcgga tcttgattac tactggttac
      361 aggaacgcgg cagcctgtgt caaccaacat acatggtagt tgatcgttct caatgagcac
      421 gggctagata aggtcgcgga agcggccttc gtcgaggaca attgaaacgc cgaaaacat
//
LOCUS       DAPHDW010000420        17970 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.guided.088, whole genome shotgun sequence.
ACCESSION   DAPHDW010000420 DAPHDW010000000
VERSION     DAPHDW010000420.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 17970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17970
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.guided.088"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            complement(122..667)
                     /gene="orn"
                     /locus_tag="RH057_004713"
     CDS             complement(122..667)
                     /gene="orn"
                     /locus_tag="RH057_004713"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HDV3130908.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEAYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
     gene            762..1814
                     /gene="rsgA"
                     /locus_tag="RH057_004714"
     CDS             762..1814
                     /gene="rsgA"
                     /locus_tag="RH057_004714"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HDV3130909.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            1911..2879
                     /gene="psd"
                     /locus_tag="RH057_004715"
     CDS             1911..2879
                     /gene="psd"
                     /locus_tag="RH057_004715"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HDV3130910.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            2901..6224
                     /gene="mscM"
                     /locus_tag="RH057_004716"
     CDS             2901..6224
                     /gene="mscM"
                     /locus_tag="RH057_004716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HDV3130911.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQISQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQEVIDNYPKLSATLRAQLNNMRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMDLVASQQRQATSQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNGQLEDALKEVNEATHRYLFWTSDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIVPLIMALMMFDNLDDRE
                     FSGSLGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVA
                     ILASAVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAE
                     MLAQRARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLW
                     SEIHSAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLE
                     LAILQHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFG
                     LQEIFANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNK
                     AFITEQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFL
                     VDLQQGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNG
                     KQTGRTLTSAGKGRQAGSL"
     gene            complement(6253..6567)
                     /locus_tag="RH057_004717"
     CDS             complement(6253..6567)
                     /locus_tag="RH057_004717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeO family protein"
                     /protein_id="HDV3130912.1"
                     /translation="MSARMFVLCCIWFIVAFLWITITSALDKEWMIDGRGINNVCDVL
                     MYLEEDDTRDVGVIMTLPLFFPFLWFALWRKKRGWFMYATALAIFGYWLWQFFLRYQF
                     CL"
     gene            complement(6564..6878)
                     /locus_tag="RH057_004718"
     CDS             complement(6564..6878)
                     /locus_tag="RH057_004718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130913.1"
                     /translation="MDDTSRDPAITEDEIRALQFSAGDVAEIEQTILSFVDACHTRKV
                     AMVVGSTINTLKDRDGKRWGNLPDIYCAYLIRCLVFRGELVGYGDLFRMRYSEIKRPV
                     TL"
     gene            complement(6930..8432)
                     /gene="yjeM"
                     /locus_tag="RH057_004719"
     CDS             complement(6930..8432)
                     /gene="yjeM"
                     /locus_tag="RH057_004719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
                     /protein_id="HDV3130914.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMMAEMGAAYRKEEGGIYSWMNNSVGPRFAFIGTFMWFSSYIIWMVST
                     SAKVWVPFSTFLYGSDMTQHWRIAGLEPTQVVGLLAVAWMILVTVVASKGINKIARIT
                     AVGGIAVMCLNLVLLLVSITILLLNGGHFAQDINFLASPNPGYQSGLAMLSFVVFAIF
                     AYGGIEAVGGLVDKTENPEKNFAKGIVFAAIVISIGYSLAIFLWGVSTNWQQVLSNGS
                     VNLGNITYVLMKSLGVTLGNALHLSPEASLSLGVWFARITGLSMFLAYTGAFFTLCYS
                     PLKAIIQGTPKALWPEPMTRLNAMGMPSIAMWMQCGLVTVFILLVSFGGGTASAFFNK
                     LTLMANVSMTLPYLFLALAFPFFKARQDLDRPFVIFKTHLSAMIATVVVVLVVTFANV
                     FTIIQPVVEAGDWDSTLWMIGGPVFFSLLAMAIYQNYCSRVAKNPQWAVE"
     gene            complement(8651..9628)
                     /gene="epmA"
                     /locus_tag="RH057_004720"
     CDS             complement(8651..9628)
                     /gene="epmA"
                     /locus_tag="RH057_004720"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HDV3130915.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            9953..11761
                     /gene="frdA"
                     /locus_tag="RH057_004721"
     CDS             9953..11761
                     /gene="frdA"
                     /locus_tag="RH057_004721"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HDV3130916.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAMNMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LGHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANKKEKANG"
     gene            11754..12488
                     /gene="frdB"
                     /locus_tag="RH057_004722"
     CDS             11754..12488
                     /gene="frdB"
                     /locus_tag="RH057_004722"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HDV3130917.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            12499..12894
                     /gene="frdC"
                     /locus_tag="RH057_004723"
     CDS             12499..12894
                     /gene="frdC"
                     /locus_tag="RH057_004723"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HDV3130918.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FGLFALKNGPEAWAGFVDFLQNPVIVIINLITLAAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            12905..13264
                     /gene="frdD"
                     /locus_tag="RH057_004724"
     CDS             12905..13264
                     /gene="frdD"
                     /locus_tag="RH057_004724"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HDV3130919.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGIVTI"
     gene            13327..14460
                     /locus_tag="RH057_004725"
     CDS             13327..14460
                     /locus_tag="RH057_004725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HDV3130920.1"
                     /translation="MFKTTLCALLITASCSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGEAIARGEIKLS
                     DPTTKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPAEEKNYAWG
                     YREGKAVHVSPGALDAETYGVKSTIEDMACWVRSNMNPRDINDKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSGNKIALAAHPVKAITPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAAWQILNALQ"
     gene            14549..15082
                     /gene="blc"
                     /locus_tag="RH057_004726"
     CDS             14549..15082
                     /gene="blc"
                     /locus_tag="RH057_004726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HDV3130921.1"
                     /translation="MRLLPLVAAATAAFLVVACSSPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(15079..15396)
                     /gene="sugE"
                     /locus_tag="RH057_004727"
     CDS             complement(15079..15396)
                     /gene="sugE"
                     /locus_tag="RH057_004727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV3130922.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(15578..15724)
                     /gene="ecnB"
                     /locus_tag="RH057_004728"
     CDS             complement(15578..15724)
                     /gene="ecnB"
                     /locus_tag="RH057_004728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HDV3130923.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(15835..15960)
                     /gene="ecnA"
                     /locus_tag="RH057_004729"
     CDS             complement(15835..15960)
                     /gene="ecnA"
                     /locus_tag="RH057_004729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HDV3130924.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTARGFGEDIKHLGNSISRAAS"
     gene            complement(16012..16578)
                     /gene="efp"
                     /locus_tag="RH057_004730"
     CDS             complement(16012..16578)
                     /gene="efp"
                     /locus_tag="RH057_004730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HDV3130925.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            16620..17648
                     /gene="epmB"
                     /locus_tag="RH057_004731"
     CDS             16620..17648
                     /gene="epmB"
                     /locus_tag="RH057_004731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HDV3130926.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLHQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKDHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     LDLQLRQQ"
BASE COUNT         4492 a         4738 c         4619 g         4121 t
ORIGIN      
        1 ccaaaaaagt tttttttgcg tttttatctc aagtgctgaa attatcagca agacgttcaa
       61 tttagcgtca aaatcacgcc gccgcaagtg gattccggcg cttacgtgac caggaaaaat
      121 tttacagctt gataaaatgc tcgcggtagt aagccagctc cgccaccgat tcacggatat
      181 catccatcgc ctgatgcgtc ccctgcttgg taaaaccatc cagaatttcc ggcttccagc
      241 ggcgcgccag ctctttcagg gtgctgacat cgagataacg gtagtggaag taggcttcca
      301 gctccggcat gtatttaaac aggaaacgac ggtcctgacc gatgctgtta ccgcaaatcg
      361 gcgattttcc cgcaggcacc cactgtttta aaaattcgag cgttgccagt tcagcttccc
      421 gatcgcccat cgtgctcgct ttcacgcgct ctaccagccc gctggcggta tgggtgcgca
      481 cgttccagtc atccatcagc gccagctgtt catcagactg gtgtactgca atggtcggcc
      541 cttctgccag aatattcagg ttggcatcgg tcaccagcgt ggcaatctca ataatgcgat
      601 cgcgctcggg atccagaccg gtcatctcaa gatcgatcca aatcaggttg ttttcattgg
      661 cactcatgct attttccacc cattcacagt gactatattg atccacaatt gcgtgtatca
      721 tagatgtttt gcccatcagg ggcgaccagg agtctgtacg attgagtaaa aataaactct
      781 ccaaaggcca gcagcgccgc gtgaacgcca atcaccagcg tcgtcttaaa acgtctaagg
      841 agaagcccga ctacgacgac aatctgtttg gcgagcctga tgaaggtatc gtcatcagcc
      901 gctttggtat gcacgctgat gtggaatccg ccgatggcga cgttcaccgc tgcaatattc
      961 gccgtaccat ccgttcgctg gtaaccggcg accgcgtagt ctggcgtccg ggtaaaccgg
     1021 cggcggaagg cgtaaatgtc aaagggatcg tggaagcggt gcatgagcgt acctcggtgt
     1081 tgacgcgtcc ggatttctac gacggcgtaa aacctattgc cgccaacatc gaccagattg
     1141 tcattgtctc cgccattttg ccggagctgt cgctcaatat tatcgaccgt tacctggtgg
     1201 cctgcgaaac cttgcagatt gagccgatta ttgtgctcaa caagatagac ctgctggacg
     1261 acgaaggcat ggcgttcgtc aacgagcaga tggatatcta ccgcaatatc ggttatcgcg
     1321 tattgatggt ttccagccat actcaggatg ggctaaaacc gctggaagag gcgttgaccg
     1381 ggcgcatcag catttttgcc gggcagtctg gcgtcggcaa atccagcctg ctgaatgcgt
     1441 tgctggggct gcaaaaagag atcctgacca acgatgtctc tgacaactcg gggctcggtc
     1501 agcacaccac gaccgccgct cggctgtatc acttcccgca cggcggtgat gtgattgatt
     1561 cccctggtgt gcgtgagttc ggcctctggc acctggagcc ggaacaaatc actcagggct
     1621 ttgtcgaatt ccatgactat ttaggtctgt gtaaatatcg cgattgcaaa cacgataccg
     1681 atccgggctg cgctatccgg gaagcggttg aggaagggaa aatcgcggaa acccgtttcg
     1741 aaaactatca ccgtattctg gaaagcatgg cgcaggtaaa aacgcgtaaa aacttttctg
     1801 atacggatga ctgacaatta agctaaccgt cgttagaatc gtcccctttt tttcaggatc
     1861 cggcatgttg gccggatcag gaacgacaaa acaatggcct ggaggctacc ttgttaaatt
     1921 catttaaact ttcgctacag tacattctgc cgaaactatg gcttactcgc ctggcgggtt
     1981 ggggcgcaag caagcgggca ggatggctga caaaactggt tatcgatctg ttcgttaaat
     2041 actacaaggt cgacatgaaa gaggcgcaaa agccggacac cgccagctac cgcaccttta
     2101 acgaattctt tgtccgtccg ctgcgtgacg aagtacgccc aatcgatacc gatccgaatg
     2161 tactggtcat gcctgccgat ggcgttatca gccagttggg taaaatcgaa gaagataaaa
     2221 tcctgcaagc caaaggccac aactacagcc tcgaagccct gctggcaggc aactatctga
     2281 tggcggacct gttccgcaac ggtacgtttg tgaccactta cctctccccg cgtgactacc
     2341 atcgcgtaca catgccgtgc aacggtattc tgcgtgagat gatctacgta ccgggcgatc
     2401 tcttctccgt taaccatctc acggctcaga acgtgccgaa tctgtttgcc cgtaacgaac
     2461 gcgtgatttg ccttttcgat accgaatttg gcccaatggc gcagattctg gtcggagcga
     2521 cgattgttgg cagcattgag acggtctggg cgggcaccat tacgccgccg cgcgaaggta
     2581 tcatcaagcg ctggacctgg cctgccgggg aaaacgacgg ttctgtggca ctgctgaaag
     2641 gccaggaaat gggtcgcttt aaactcggtt ccaccgttat caacctgttt gcaccgggta
     2701 aagtgaatct ggttgagcaa ctggaaagcc tgtctgtcac gaaaattggc cagccgctgg
     2761 cagtatctac cgaaaccttt gttacgccag acgctgaacc ggccccgctt cctgctgaag
     2821 agatcgaagc agaacacgac gccagcccat tggttgacga taaaaaagac caggtctaat
     2881 ccatcaaagg aaacgctgac gtgcgcctga ttatcacttt tctgatggcc tggtgcctca
     2941 gttggggggc gtacgccgcg acggcccccg atagcaaaca aatctcacaa gaactggagc
     3001 aggcaaaagc ggcgaaaccc gcgcagccgg aagtcgtaga ggcgctccag tccgccttaa
     3061 atgcgcttga ggaacgaaaa ggttcccttg agcgcatcaa gcaatatcaa gaagtcattg
     3121 ataattaccc gaaactttcc gctactctgc gcgcacaatt aaacaacatg cgtgacgaac
     3181 cgcgcagcgt gtcgccgggg atgtctaccg acgcgctgaa tcaggaaatt ctccaggtca
     3241 gcagccagtt gctggataaa agccgtcagg cccagcaaga gcaggagcgc gcccgcgaga
     3301 ttgccgattc gctgaatcaa ctgccgcaac agcaaaccga cgcccgccgt cagttaaatg
     3361 agatcgagcg ccgcctggga acgcttactg gcaatacccc gctcaatcag gcacaaaatt
     3421 tcgcgttgca gtctgactcc gcacgcctga aagcgctcgt tgatgaactg gaactggcgc
     3481 agctgtctgc caataaccgt caggaattag cgcgcttacg ctcagagctg gcggaaaaag
     3541 agagccagca actggatgcg tatttgcagg ccttacgtaa tcagttgaac agccaacgtc
     3601 agcttgaggc ggagcgggcg ctggaaagta ccgaattgct ggcagaaaac agcgccgatt
     3661 tgccgaaaga catcgtcgcg caattcaaaa ttaaccgcga actatcggcg gcactgaatc
     3721 aacaggcgca gcggatggat ctcgttgcct cgcaacagcg ccaggccacc agccagacgt
     3781 tacaggttcg gcaggcgctg aatacgctgc gtgaacagtc gcaatggctg ggatcgtcca
     3841 acctgctcgg cgaagcgttg cgggcgcagg tggcacggct gccggaaatg ccgaaaccac
     3901 aacagcttga taccgaaatg gcgcagttgc gtgtgcaacg gttacgttat gaggatctgc
     3961 ttaataaaca gccgctgcta cggcaaattc atcaggccga cggtcagccg ctgaccgccg
     4021 agcaaaaccg tattctggaa gcacagctac gcactcagcg tgagttgctg aactcattgt
     4081 tgcagggtgg cgacacgcta ctgctggaac tgaccaagct gaaagtctcc aacgggcaac
     4141 ttgaggatgc gctgaaagag gtgaatgaag cgacgcatcg ctatctgttc tggacctctg
     4201 acgtgcgccc gatgaccatc gcctggccac tggaaatcgc ccaggatctg cgtcgtctca
     4261 tttcgctgga caccttcagt cagttgggca aagccagtgt gatgatgctg accagcaaag
     4321 agacgatttt gccgctgttt ggcgcgttga ttctggtcgg ttgcagtatt tactcgcgcc
     4381 gctatttcac ccgttttctt gaacgttcgg cggcgaaagt cggcaaagtg actcaggatc
     4441 acttctggct gacgttgcgc actcttttct ggtcgattct cgtcgcgtca ccgctgccgg
     4501 tgctgtggat gacgctgggt tacggcttgc gcgaggcgtg gccttatccg ctggcggtcg
     4561 cgattggcga tggcgtaacg gccaccgtgc cgctgctgtg ggtagtgatg atttgcgcca
     4621 cctttgcccg cccgaacggc ttgtttatcg ctcattttgg ctggccgcgc gaacgtgttt
     4681 cccgtgggat gcgctactac ctgatgagca tcgggcttat tgtgccgctg attatggcgc
     4741 tgatgatgtt cgataacctc gacgaccgtg aattctccgg ttcgctggga cggctttgct
     4801 ttatcctcat ttgcggtgcg ctggcggtgg tcaccctcag cctgaaaaag gccgggatcc
     4861 cactgtatct caacaaagag ggcagcggcg acaacattac caaccatatg ctgtggaaca
     4921 tgatgattgg cgcgccgttg gttgccattc tggcgtcggc tgtgggttat ctggcaacgg
     4981 cacaggcgct gttagcgagg cttgaaacct cggttgccat ctggttcctg ctactggtgg
     5041 tttatcacgt tatccgccgc tggatgctga tccagcgtcg caggctggcg tttgaccggg
     5101 cgaagcatcg ccgggcagag atgttagcgc aacgcgcgcg tggcgaagag gaagcgcatc
     5161 atcacagtag cccggaaggg gcaattgaag tcgatgaaag cgaagtcgat ctcgatgcca
     5221 tcagtgcgca atccttgcgg ctggtgcgct caattttgat gttgatcgcc ctgctttcgg
     5281 tcattgtgct gtggtcagaa atccattccg ctttcggctt cctcgaaaat atttctctgt
     5341 gggatgtcac ttccactgta cagggcgtag aaagcctgga gccgattacc ctcggtgcag
     5401 tgctaattgc cattctggta tttatcatca ccacgcagct ggtgcgcaac ctgccagcgc
     5461 tgctggaact ggcgattttg cagcacctgg atttaactcc gggtacgggt tacgccatca
     5521 ccaccatcac caaatatctg ctgatgctga ttggcgggct ggtcggcttc tcaatgattg
     5581 gtattgagtg gtcgaaattg cagtggctgg ttgccgcgct cggtgttggt ctcggttttg
     5641 gtttgcagga aattttcgcc aactttatct ctggtctgat tatcctgttc gaaaaaccga
     5701 ttcgcattgg cgatacggtg acaattcgcg atctcaccgg tagcgtgacg aaaattaaca
     5761 cccgcgccac caccatcagc gactgggacc gtaaagagat aatcgtgccg aacaaggcgt
     5821 ttattaccga gcagtttatc aactggtcgc tctctgactc ggtcacgcgc gtggtgttga
     5881 cgataccggc ccctgccgat gccaatagcg aagaagtgac ggaaatcctg ctcaccgcag
     5941 cgcgtcgctg ctcgctggtg atcgacaacc cggcaccgga agtcttcctg gtggatctgc
     6001 aacaggggat tcagattttc gagctgcgta tttacgccgc tgagatgggt caccgtatgc
     6061 cgctacgcca tgagatccac cagctgattc tggcaggctt ccacgcccac ggtatcgata
     6121 tgccattccc gcccttccag atgcgtctgg aaagcctcaa cggtaaacaa acggggagaa
     6181 cgctgacgtc tgcgggcaaa ggtcgtcagg cgggaagttt gtaagtttgt gtgccggatg
     6241 aaccaatccg gctcacaaac aaaactgata gcgcagaaaa aactgccaca gccagtagcc
     6301 gaaaatagcc agcgcggtgg cgtacataaa ccagccgcgt ttttttcgcc acaaagcgaa
     6361 ccagagaaaa gggaaaaaga gcggtaacgt cattatcaca ccaacatcac gggtatcgtc
     6421 ttcttcgaga tacatcaaca catcgcagac gttgttaatc ccgcgtccat caatcatcca
     6481 ctctttgtcc agcgcggaag tgatggttat ccagaggaac gccacaataa accagatgca
     6541 gcacaagaca aacatgcgcg cactcataag gtaaccggtc gcttaatttc gctatagcgc
     6601 atacgaaaca aatcgccgta tcccactaat tccccgcgaa aaaccagaca tcggataaga
     6661 taagcgcaat aaatatccgg caggtttccc caccgtttac catcgcgatc tttgagggtg
     6721 ttaatggtgc tacccaccac catcgccact ttacgggtgt ggcaggcatc gacaaaagag
     6781 agaatcgtct gctctatttc cgctacatcc ccggcagaaa attgcagcgc cctgatttca
     6841 tcttcggtta ttgccgggtc ccggctggtg tcatccataa catttcctgt ctttacgccc
     6901 tatcgtcagg cagacgatag ggcatcgggt tattccaccg cccactgcgg gttttttgcc
     6961 actctgctgc aatagttctg gtaaatcgcc atcgctaaca gcgagaagaa gacagggccg
     7021 ccaatcatcc acaatgtgct gtcccagtct ccggcttcaa ccacaggctg aataatggtg
     7081 aagacgttcg caaatgtcac caccagtacg acaaccactg tcgcaatcat tgccgacaaa
     7141 tgcgttttga aaatcacaaa tggtctgtcg agatcctgac gtgctttaaa gaatgggaaa
     7201 gccagcgcga ggaacaggta aggaagcgtc atagacacgt tcgccatcag cgtcagcttg
     7261 ttaaagaacg cggatgcggt accgccacca aacgaaacca gcaggatgaa gacagtaacc
     7321 aacccgcact gcatccacat ggcgatagac ggcatcccca tcgcattcag gcgcgtcatc
     7381 ggttccggcc acaacgcttt cggcgtcccc tggatgatgg ctttcagcgg tgaatagcaa
     7441 agcgtaaaga acgcaccggt ataagcgagg aacatcgaaa gtccggtaat acgcgcaaac
     7501 catacgccca gcgacaatga cgcttcaggt gacaaatgca gtgcgttacc cagcgtcacc
     7561 ccgaggctct tcatcagcac ataggtaata ttgccaaggt taacggaacc attacttaat
     7621 acctgctgcc agtttgtgct gacaccccat aaaaatattg ccagcgaata accgattgaa
     7681 ataacaatag cggcaaaaac aatacctttg gcaaagttct tttctggatt ttccgtttta
     7741 tcgaccagac caccaaccgc ttcaattccg ccataggcaa aaatagcaaa taccacaaac
     7801 gatagcattg ccagaccgga ctgataacct gggttcggtg atgcaaggaa attaatatcc
     7861 tgcgcgaaat gcccaccatt taataacaaa atagtaatgc ttactaacag caatactaaa
     7921 ttcagacaca ttactgcaat accgcccacc gcagtaatgc gggcaatttt attaatcccc
     7981 tttgaagcaa cgacggtgac cagaatcatc catgccactg ccagcagacc aaccacctgc
     8041 gtaggctcca gtccagcaat acgccagtgc tgggtcatgt cgctaccata gaggaatgtt
     8101 gagaacggta cccaaacttt cgcggaggta ctcaccatcc agatgatata agaggaaaac
     8161 cacataaacg taccaatgaa ggcaaaacgt ggtccgacac tattattcat ccaggaatag
     8221 ataccgcctt cttctttgcg ataagcagct cccatttcag ccatcattaa ggcgaatgga
     8281 ataaagaata acaatgcaga aaatatataa aagggaatcg cactataacc cattaagtaa
     8341 taagccgatg ggctattggc aaatccaaat acggaagtaa agatcatcaa tatgagtcct
     8401 atcagactca tcttttttat cgtgtgaggc atgaaaccat ccttcatttc agcgcgtgta
     8461 taacacgtgc ccactgaact gcactgcgca acattgagca ggcagcgtaa aaaccaatac
     8521 agaattgata cctggatgat atcaaatatc cagtcttaaa ttgtggcctt taacggaaaa
     8581 atgaaaacat atgctacaga atggcgctta tcacgccatt cttcgctgtt aattcagtaa
     8641 tttttcagaa ttatgcccgg tcaacgctaa aggcgatgac ttcagccagt gtctccgcgc
     8701 ccagcgccaa catcaccaga cgatcaacac ctaatgccac gccggaacag tcaggcatac
     8761 cgactttcaa ggcttcaatc agattctggt caatggggtg ctgcggcaaa ccgcgcgccg
     8821 cgcgcttacg gttatcttgt tcaaagcgtt gttgctgctc acgggcatcc gtcaattcat
     8881 ggaaaccatt cgccagctca atacctttat aataaacctc aaagcgttca gcgacccgat
     8941 gatcttcggt actgatttgc gccagtgatg cctggctggc tggaaagtgg tacacaaagg
     9001 tcggtttttc tttgccaata tttggctcta cgccaaaggt aaacagcaat tgtagcagcg
     9061 tgtcgcggtc ttcttcggta tcagcaacat tgctcaaatc cagtttcgct gcgacttccc
     9121 gcagttgcgt tttgtcggca gagagcgggt caatttccag ataacgcaag aaagcttgtt
     9181 gataagaaag gctttctgct gccgggcagt ccagcacctg ttgtaagaga tcgtccacct
     9241 cgttcatcaa ccggtacata tcatagtgcg gtcgatacca ctccagcata gtgaactcag
     9301 ggttgtgata acgccccatc tcttcattac ggaagctgcg gcacagctgg aataccggcc
     9361 cacaaccggc aaccagcagg cgtttcatat ggtattccgg gctggtcatt aaccagagat
     9421 tcatcccctg cgaatgcccg gggccaacga aacgtgtctc aaacgggacc aaatgaatat
     9481 cggttaccgt cgcctggctc atacaaggcg tctccacctc cagcactcca cgatcggcaa
     9541 agaaacgacg gatctccgcc ataatcgccg cgcgttttaa taagttagga atggatgcgc
     9601 tcggctgcca ggatgccgtt tcgctcatag ttaaatctcc agtttttgac aagggcacga
     9661 agtctactcg caacgcgacg gcgagacaaa ttttacgcag gaatcaaaca gcggtgggca
     9721 gtgactaaaa aaagcacgat ctgatggttt agtaattaaa ttaatcatct tcagtgataa
     9781 tttagccctc ttgcgcacta aaaaaatcga tctcgtcaaa tttcagactt atccatcaga
     9841 ctatactgtt gtacctataa aggagcagtg gaatagcgtt cgcagaccgt aactttcagg
     9901 cacttaccct gaagtacggg gctgtgggat aaaaacaatc tggaggaatg tcgtgcaaac
     9961 ctttcaagcc gatcttgcca ttgtaggcgc cggtggcgcg ggattacgtg ctgcaattgc
    10021 tgccgcgcag gcaaatccaa atgcaaaaat cgcactaatc tcaaaagtat acccgatgcg
    10081 tagccatacc gttgctgcag aagggggctc cgccgctgtc gcgcaggatc atgacagctt
    10141 cgaatatcac tttcacgata cagtagcggg tggcgactgg ttgtgtgagc aggatgtcgt
    10201 ggattatttc gtccaccact gcccaaccga aatgacccaa ctggaactgt gggggtgccc
    10261 atggagccgt cgcccggatg gtagcgtcaa cgtacgtcgc ttcggcggca tgaaaatcga
    10321 gcgcacctgg ttcgccgccg ataagaccgg cttccatatg ctgcacacgc tgttccagac
    10381 ctctctgcaa ttcccgcaga tccagcgttt tgacgaacat ttcgtgctgg atattctggt
    10441 tgatgatggt catgttcgcg gcctggtagc aatgaacatg atggaaggca cgctggtgca
    10501 gatccgtgct aacgcggtcg ttatggctac tggcggtgcg ggtcgcgttt atcgttacaa
    10561 caccaacggc ggcatcgtta ccggtgacgg tatgggtatg gcgctaagcc acggcgttcc
    10621 gctgcgtgac atggaattcg ttcagtatca cccaaccggt ctgccaggtt ccggtatcct
    10681 gatgaccgaa ggctgccgcg gtgaaggcgg tattctggtc aacaaaaatg gctaccgtta
    10741 tctgcaagat tacggcatgg gcccggaaac tccgctgggc gagccgaaaa acaaatatat
    10801 ggaactgggt ccacgcgaca aagtttctca ggccttctgg cacgaatggc gtaaaggcaa
    10861 caccatctcc acgccgcgtg gcgatgtggt ttatctcgac ctgcgtcacc tcggcgagaa
    10921 aaaactgcat gaacgtctgc cgttcatctg cgaactggcg aaagcgtacg ttggcgtcga
    10981 tccggttaaa gaaccgattc cggtacgtcc gaccgcacac tacaccatgg gcggtatcga
    11041 aaccgatcag aactgtgaaa cccgcattaa aggtctgttc gccgtgggtg aatgttcctc
    11101 tgttggtctg cacggtgcaa accgtctggg ctccaactcc ctggcggaac tggtggtctt
    11161 cggccgtctg gccggtgaac aagcgacaga gcgtgcagca actgccggta atggcaacga
    11221 agcggcaatt gaagcgcagg cagctggcgt tgaacaacgt ctgaaagatc tggttaacca
    11281 ggatggcggc gaaaactggg cgaagatccg cgacgaaatg ggcctggcaa tggaagaagg
    11341 ttgcggtatc taccgtacgc cggaactgat gcagaaaacc atcgacaagc tggcagagct
    11401 gcaggaacgc ttcaagcgcg tgcgcatcac cgacacctcc agcgtgttca acaccgacct
    11461 gctctacacc attgaactgg gccacggtct gaacgttgct gaatgtatgg cgcactccgc
    11521 aatggcacgt aaagagtccc gcggcgcaca ccagcgtctg gacgaaggtt gcaccgagcg
    11581 tgacgacgtc aacttcctca aacacaccct cgccttccgc gatgctgatg gcacgactcg
    11641 cctggagtac agcgacgtga agattactac gctgccgcca gctaaacgtg tttacggtgg
    11701 cgaagcggat gcagccgata aggcggaagc agccaataag aaggagaagg cgaatggctg
    11761 agatgaaaaa cctaaaaatt gaggtggtgc gctataaccc ggaagtcgat accgcaccgc
    11821 atagcgcatt ctatgaagtg ccttatgacg caactacctc attactggat gcgctgggct
    11881 acatcaaaga caacctggca ccggacctga gttaccgctg gtcctgccgt atggcgattt
    11941 gtggttcctg cggcatgatg gttaacaacg tgccaaaact ggcatgtaaa accttcctgc
    12001 gtgattacac cgacggtatg aaggttgaag cgttagctaa cttcccgatt gaacgcgatc
    12061 tggtggtcga tatgacccac ttcatcgaaa gtctggaagc gatcaaaccg tacatcatcg
    12121 gcaactcccg caccgcggat cagggtacta acatccagac cccggcgcag atggcaaagt
    12181 atcaccagtt ctccggttgc atcaactgtg gtctgtgcta cgccgcgtgc ccgcagtttg
    12241 gcctgaaccc agagttcatc ggtccggctg ccattacgct ggcgcatcgt tataacgaag
    12301 atagccgcga ccacggtaag aaggagcgta tggcgcagtt gaacagccag aacggcgtat
    12361 ggagctgtac tttcgtgggc tattgctccg aagtctgccc gaaacacgtc gatccggctg
    12421 cggccattca gcagggcaaa gtagaaagtt cgaaagactt tcttatcgcg accctgaaac
    12481 cacgctaagg agtgcaacat gacgactaaa cgtaaaccgt atgtacggcc aatgacgtcc
    12541 acctggtgga aaaaattgcc gttttatcgc ttttacatgc tgcgcgaagg cacggcggtt
    12601 ccggctgtgt ggttcagcat tgaactgatt ttcgggctgt ttgccctgaa aaatggcccg
    12661 gaagcctggg cgggattcgt cgacttttta caaaacccgg ttatcgtgat cattaacctg
    12721 atcactctgg cggcagctct gctgcacacc aaaacctggt ttgaactggc accgaaagcg
    12781 gccaatatca ttgtaaaaga cgaaaaaatg ggaccagagc caattatcaa aagtctctgg
    12841 gcggtaactg tggttgccac catcgtaatc ctgtttgttg ccctgtactg gtaaggagcc
    12901 tgagatgatt aatccaaatc caaagcgttc tgacgaaccg gtattctggg gcctcttcgg
    12961 ggccggtggt atgtggagcg caattattgc gccggtgatg atcctgctgg tgggtattct
    13021 gctgccactg gggctgtttc cgggtgatgc gctgagctac gagcgcgttc tggcgttcgc
    13081 gcagagcttc attggtcgcg tattcctgtt cctgatgatc gttctgccgc tgtggtgtgg
    13141 tttacaccgt atgcaccacg cgatgcacga tctgaaaatt cacgtgcctg cgggcaaatg
    13201 ggttttctac ggtctggctg ctatcctgac agttgtcacg ctgattggta tcgttacaat
    13261 ctaacgtatc gccaatgtaa atccggcccg cctatggcgg gccgttttgt atggaaacca
    13321 gaccttatgt tcaaaacgac gctctgcgcc ttattaatta ccgcctcttg ctccacattt
    13381 gctgcccctc aacaaatcaa cgatattgtg catcgcacaa ttaccccgct tatagagcaa
    13441 caaaagatcc ccggtatggc ggtggcggta atttatcagg gtaaacctta ttactttacc
    13501 tggggctatg cggacatcgc caaaaagcag cccgtcacac agcaaacgtt gtttgagtta
    13561 ggttcggtca gcaaaacatt tacgggcgtg cttggtggcg aggctattgc tcgaggggaa
    13621 atcaagttaa gcgatcccac aacaaaatac tggcctgaac ttaccgctaa acagtggaat
    13681 gggatcacac tattacatct cgcaacctac actgctggcg gcctgccatt gcaggtgccg
    13741 gatgaggtga aatcctcaag cgacttgctg cgcttctatc aaaactggca gcctgcatgg
    13801 gcgccaggaa cacaacgtct gtatgccaac tccagtatcg gtttgttcgg cgcactggct
    13861 gtgaagccgt ctggtttgag ttttgagcag gcgatgcaaa cacgtgtctt ccagccactc
    13921 aaactcaacc atacgtggat taatgtaccg cccgcagaag aaaagaatta cgcctgggga
    13981 tatcgcgaag gcaaggcagt tcatgtttcg ccaggggcgt tagatgctga aacttatggt
    14041 gtgaagtcga ccattgaaga tatggcctgc tgggtacgaa gcaatatgaa tccccgtgat
    14101 atcaacgaca aaacacttca gcaagggata caactggcac aatctcgcta ctggcaaacc
    14161 ggcgatatgt atcagggcct gggctgggaa atgctggact ggccggtaaa tcctgacagc
    14221 atcattaacg gcagtggcaa taaaattgca ctggcagcac accccgtaaa agcgattacg
    14281 cccccaactc ctgcagtacg cgcatcatgg gtacataaaa caggggcaac cggcggattt
    14341 ggtagctatg tcgcgtttat tccagaaaaa gagctgggta tagtgatgct ggctaacaaa
    14401 aactatccca atccagcgag agtcgccgcc gcctggcaga ttctcaacgc tctacagtaa
    14461 aatttcatcg ggtccgaatt ttcggacctt ttctgcgctt ttccttgctg tcatctacac
    14521 ttagaaaaaa accagtaagg aaacatttat gcgcctgctc cctctcgttg ccgcagcgac
    14581 agctgcattt ctggtcgttg cctgcagttc tcctacgccg ccgcgtggcg taacagtagt
    14641 gaataatttc gacgccaaac gttatctcgg tacctggtat gagattgccc gttttgatca
    14701 ccgttttgaa cgtggactgg aaaaagtcac cgcaacatac agcctgcgtg atgacggcgg
    14761 cctgaatgtc attaataaag gctataaccc tgacagagga atgtggcagc agagtgaagg
    14821 gaaagcgtac tttaccggcg caccaactcg cgctgcgctg aaagtgtcat tctttggtcc
    14881 tttctatggc ggttataacg ttattgcact cgatcgggaa taccgccatg cgctggtttg
    14941 cggcccggac cgcgactacc tgtggatact ctcccgcacg ccaaccattt ctgacgaagt
    15001 gaaacaggag atgctggcag tcgcgacccg ggaagggttt gatgtcagta agtttatttg
    15061 ggtacagcag cccggtagtt agtgagtgct gagtttcaga ccaataatcc ccaatacgat
    15121 taacgccaga ctcgccaggc gcatcgggtt agcggactca ccgagcagca caatgccggt
    15181 gatagccgcg ccgactgcgc caatacccgt ccacacggca taagccgtcc ctactggtaa
    15241 cgatttcatc gcccaggcaa gtagcgccat actgacaatc atcgccgtca cggtaataac
    15301 actcggcgtc aaacgactaa agccgtgggt atatttcagg ccaacggccc ataccacttc
    15361 cagcagacca gcaataacta agataatcca ggacatatca ggctccggag aattggggcc
    15421 gtccccggtg aaaagaggcg tttgcaggtc gtcctgcaaa gctgatggtt gaacggatat
    15481 tttgccaggt ggcaggggaa tttcaactat taccttagtg actatcgcag aaaaaacaaa
    15541 cggcacgaca tacctgtcgt accgttattg cttattgcta ttgctgcgct ttcgttgctg
    15601 cgccagaaat cgcgttaccg ccatcagaaa tgtcttcacc aacgccacgc gtggtgttgc
    15661 aggcagttaa tactgttgaa agcaccagaa cagaaaagat cgctgcaatt gtcttcttca
    15721 ccataacgtc ttccttttag gtaatgttat ttatgtttgc ctatagcaaa ttaataatag
    15781 acaacatcgc caaaaatgac ggaaatctga tgatttttcg gaagagaaga aaaattagct
    15841 ggcagcgcga gagatggagt tgccgagatg tttgatgtct tcgccgaaac cgcgagcggt
    15901 gttacagccc gtgagcagcg tgctggcaag caaaacaaga acgataaggc gtttcatcat
    15961 ctctgacctg aataagtgat ggtgcagcct gcaggccgca ccacaaccgc attacttcac
    16021 gcgagagacg tattcaccag agcgggtatc cactttgatg acttcgccga tttgtacgaa
    16081 cagcggaacc ttaaccacag cgccagtaga cagggtagcc ggtttgccgc cagtacctgc
    16141 ggtatcacct ttcaggcccg gatcggtatc aacgatttcc agttcaacga agttcggcgg
    16201 agtaacggag atcggctgac cattccacag agttacgata cactctgcct gatccagcag
    16261 ccatttagcg ttgtcgccaa ttgcttttgc atcagcagac agctgctcga aagtttcgtt
    16321 gttcatgaag tgccagaact caccgtcgtt gtacaggtaa gtcaggttca tatcgacaac
    16381 atcagcgcct tcagcggaat cagtagattt gaaggttttt tctacgcgag taccggtcag
    16441 cagacgacgc agtttaacgc gagcaaatgc ctggccttta cccggtttta cgaattcact
    16501 cgcttcaacc gcgtaaggtt cgccgtctaa catgatttta agaccagcac gaaaatcgtt
    16561 gctatagtac gttgccataa ggccctctga aatttgttaa ttggtagcta agccacaaaa
    16621 tggcgcatat tgtaacccta aataccccat ccagagaaga ttggttaacg caacttgccg
    16681 atgttgtgac cgatcctgat gaacttctgc gtcttttgaa tatagacgcg gacgaaaaac
    16741 tgttggccgg acgcagcgcc aaaaagctgt ttgccctgcg tgtgccccgc tcatttatcg
    16801 atcgcatgga gaaaggcaat ccggacgatc ctcttttgca tcaggtactt acctcgcaag
    16861 atgagtttgt cgtcgcgtcc ggattctcca ccgacccgct ggaagaacag cacagcgtag
    16921 tgcctggttt gttgcataaa taccacaacc gggcgctttt gctggtcaaa ggcggctgcg
    16981 cggtaaattg ccgctattgc ttccgtcgcc acttccccta tgccgaaaat cagggcaaca
    17041 agcgtaactg gcagactgct cttgagtatg ttgctgcgca tccggaactg gacgagatga
    17101 ttttctccgg cggcgatccg ctgatggcga aagatcacga gctggactgg ttgctcacac
    17161 aactggaagc catcccgcat atcaaacgtc tgcggattca cagccgtctg ccgattgtga
    17221 tcccggcacg tatcaccgag gcgctggttg aacgctttgc ccgttctacg ctgcaaatct
    17281 tgctggtgaa tcacatcaat catgccaatg aggtagatga aacgttccgt caggcgatgg
    17341 ctaaattgcg ccgtgtcggt gtcaccctgc ttaaccagag cgttctgtta cgtggtgtga
    17401 acgataacgc acaaacgctg gcaaacctga gtaatgcgtt gttcgatgcc ggcgtaatgc
    17461 cgtattacct gcatgtgctc gataaagtac agggcgcggc gcattttatg gtgagtgatg
    17521 acgaagcacg gcagattatg cgtgagttgc tgacactggt gtcgggttat ctggtgccga
    17581 aactggcgcg agaaatcggc ggcgaaccca gcaaaacgcc gctggatctc cagctacgcc
    17641 agcagtaact gattaaacag ggaagttaaa cattagcctg atgcgctacg cttatcaggc
    17701 ctacattgtg cattgaaatt gaatggattt gcaatgaact gtaggccgga taaggcgttt
    17761 acgccgcatc cggcaagagc aacaaacgca agtacagcaa cctgtcatta aactgggaaa
    17821 tttaatcttt gcccgttgca taacctttaa ttattcatat acattgctgt tgttactctt
    17881 tatatagcca acaccaataa ttaacctttc ttttctgttt atatagagtg agtacggcta
    17941 tttatgttat tttaacaact attactacca
//
LOCUS       DAPHDW010000421        14107 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.guided.121, whole genome shotgun sequence.
ACCESSION   DAPHDW010000421 DAPHDW010000000
VERSION     DAPHDW010000421.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 14107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14107
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.guided.121"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            306..1451
                     /locus_tag="RH057_004732"
     CDS             306..1451
                     /locus_tag="RH057_004732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C beta-lactamase CMY-2"
                     /protein_id="HDV3130927.1"
                     /translation="MMKKSLCCALLLTASFSTFAAAKTEQQIADIVNRTITPLMQEQA
                     IPGMAVAVIYQGKPYYFTWGKADIANNHPVTQQTLFELGSVSKTFNGVLGGDAIARGE
                     IKLSDPVTKYWPELTGKQWQGIRLLHLATYTAGGLPLQIPDDVRDKAALLHFYQNWQP
                     QWTPGAKRLYANSSIGLFGALAVKPSGMSYEEAMTRRVLQPLKLAHTWITVPQNEQKD
                     YAWGYREGKPVHVSPGQLDAEAYGVKSSVIDMARWVQANMDASHVQEKTLQQGIALAQ
                     SRYWRIGDMYQGLGWEMLNWPLKADSIINGSDSKVALAALPAVEVNPPAPAVKASWVH
                     KTGSTGGFGSYVAFVPEKNLGIVMLANKSYPNPVRVEAAWRILEKLQ"
     gene            1545..2078
                     /locus_tag="RH057_004733"
     CDS             1545..2078
                     /locus_tag="RH057_004733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin family protein"
                     /protein_id="HDV3130928.1"
                     /translation="MRILPVVAAVTAAFLVVACSSPTPPKGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLDKVTATYSLRDDGGINVINKGYNPDREMWQKTEGKAYFTGDPSRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEMKQQMLAIAT
                     REGFEVNKLIWVKQPGA"
     gene            complement(2075..2392)
                     /gene="sugE"
                     /locus_tag="RH057_004734"
     CDS             complement(2075..2392)
                     /gene="sugE"
                     /locus_tag="RH057_004734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HDV3130929.1"
                     /translation="MSWIVLLIAGLLEVVWAIGLKYTHGFTRLTPSIITIAAMIVSIA
                     MLSWAMRTLPVGTAYAVWTGIGAVGAAITGILLLGESASPARLLSLGLIVAGIIGLKL
                     STH"
     gene            3120..8606
                     /locus_tag="RH057_004735"
     CDS             3120..8606
                     /locus_tag="RH057_004735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4165 domain-containing protein"
                     /protein_id="HDV3130930.1"
                     /translation="MINFKPKIPAMLGALAVLTAGAAHAELLEYTFKAPDGTQRSLTP
                     NANYANPTGNISFALSAGIDRKVKISVLRSDGTVVSTATSHLLGATDRITVGGKSYYG
                     AELQLPAPVGGAYTIRAEILASDGSTVQTDEYPLTVDVTPPTYSSLAPVYSNYGQVTS
                     GDVWKLGLGGSEDNAFLLSGISDESPIKGVKAKLYRQDGSLYKDVSVNYDDANGQARQ
                     SFQSGFFPASDLDEVFTLQFEISDSAGNSYLSPRQKVMFDSITNAPSAPFGVYDPSST
                     NNLGPGLTGFVAYTEGMTVKTNPIKLAWRVPRDNWHEYREGGINMTNALGEMSKVGED
                     ASYVYLVTTAPYGNTDGNYWRWVNFGQWGGGGIAYNLTLSPSAPKSPRLLGVDYNYSD
                     IGWSSFYRYWVNNSVLPVTVSSIRVKVEPRPYVQTAVHRGSCEIPVGQDSCVIANSFT
                     MAKGTTGYVHDNATVFNPDKSLRSNPLWAEVNWNDQHYPQLSQQFDQNSKVFTLFVNQ
                     PGRGAYFDRLRLRSAWIEDSKGNKLSPTGGLIANNWENYTYQWDLKTLPEGQYSLVAA
                     AEEMHGPLTRQPMFQITSDRTPPTMTLSVADGAAIQTLDDVVITLADAIDPSPKLTSI
                     ALVGGPANDKVQLSWREESKGRFRLEYPVMFPSLKEGESYTLTVSGEDAQGNAVQKAV
                     GFEYKPRQVMLADGMDGKVMVPAVTHEFVHADGKRIIETKPLTLSDGAVVTGSYDVFA
                     TLRSDAKVPLVVNGVRIEPGQTMGIMSQHDFGASGGRLSIPVKPAVPDVVGSSSLLVM
                     TSAPNSPILVVDINTWKGTAKLSAESWTIRQVIDPVKIYALPESGVPCRFTTKEDVAM
                     AADPIRDPVCLLQWDRTPDEAEQTTQDNNGMKVAGLVGQAVSIGEQPVEYSLYLFSGD
                     GSKVKVGSGSQNLTVTTAYGSVGYTPIDDIAQVNRVIEDFDVNFKQSKGPDCSITLSA
                     DRAKKEAANKAVGSASRACLFEWQQIPDGLVQDPLSESPSLSGSLASNGVHPLGWRVS
                     IFTRNGTRVTLNDETFNVEAVDPPAPTVELASDYNFKDNIYLVPMTGNYLGDAIINSE
                     RADLDIAISRNSDVLESETFTPGWGATNKVYRRINTDERALWEETTYKVNAAYNKVPD
                     VKTEVVYRAISAPSDSIRPIVEVKGDTAIDTQALPVRVLIRDQYKPDGDYDANTMGVW
                     KVRLIQQKAYNETVALTDYAEASNGEAQFSVDLSGVDTSSVRIAAEAVLESPVEGYNR
                     TELSIRPAFLTVLRGGAIGAGVEARKLSGEAPFTAVFKLSLDDRQDLRATGQVVWETS
                     KDDGKTWEQFIPEDRYKYQLVKTFDKGEYQVRAKVVNVNSGAEKYTEAVSVVAYDKPD
                     IAVIGPTTLFVGSEGKYTANLTLNDEPISGGNAIVEWSTDGGKTYAQTGDSITLSSDE
                     ETRYRLWARVRSATAPADDGYAYEVAKTAVDFRAVKAPRPYVTGPRVIETGKKYVFKA
                     ETSLPYRGMDVKLNGFFTLPDGSIVQGDTAEYEPSDTDLNQATVETKYTTWIEGYRDQ
                     GAEASHSLRSRVWQYVWPSFGMQVRKNADVAPATITASVRPIAFNGKLEEPTYEWELP
                     EGAVIQDQRQDIVRSFVINEPGDYNIKVTVRDARGHETVIEQPLKIGQAAPYAIDLQY
                     SGSNKYEREPLDVLLRPYISGGHPRDRISTRVYSVDGTPLESSGYYGRATLGAGEHSI
                     KLKITSEMGHEAEGEVNINVAENKLPACSLSSRETVGSWIVYANCEDTDGRMKSYEWT
                     IAGELQSISSDRVTISKGTYETMPTISLVGVDDSGGKSEAVTMN"
     gene            8755..9462
                     /locus_tag="RH057_004736"
     CDS             8755..9462
                     /locus_tag="RH057_004736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001259344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbC family protein"
                     /protein_id="HDV3130931.1"
                     /translation="MRTKLLGALMVFGIITGTAHASSKLEITDPRAAKIEDIVELPIK
                     GVRAVQSDGQIMFLSENGRFVISGQIYDLWSKKPLNTMSQMRDVAERIHFKSMGMDVD
                     TLNTVSMGRGDKEVVVFVDPRCAVCHQLMGDAKSLVDDYTFKFIVIPALGAESNRLAK
                     NLYCAKDKTHALDALMNNTLGSLPSKETCDPGQYDQTLLTAHFIGIEGVPFVVAPDGR
                     VSKGRPKNLKSWLESAE"
     gene            9459..10160
                     /locus_tag="RH057_004737"
                     /pseudo
     CDS             9459..10160
                     /locus_tag="RH057_004737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000637386.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraC"
     gene            10461..10805
                     /locus_tag="RH057_004738"
     CDS             10461..10805
                     /locus_tag="RH057_004738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ProQ/FinO family protein"
                     /protein_id="HDV3130932.1"
                     /translation="MTKRQRKNRKRINRLVEHWPELFNREKPQPRKVEIPDDLIQDIA
                     IRELAFSAGALRAAVASYVQSPRYYRALMAGGARYDLKGQPCGEVTPQEQKEAETRLM
                     VLNDRQKARKPR"
     gene            complement(10824..11372)
                     /locus_tag="RH057_004739"
     CDS             complement(10824..11372)
                     /locus_tag="RH057_004739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001167424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130933.1"
                     /translation="MQITEALISEPGDIRRFVQQAVDHWPNLLAFHFTLYSAEGNING
                     QQIHAFCTSFYRQVHERITERNHTACPSSPVVLRWLREQHGGATIRCLLLLSQTSICH
                     PRASATVDEECSQRVDLLQQTWKVISAGGQCQVERCFRVARGDTSGQYVALKTVALSL
                     GLPVVTAITHRPVQRCTLITAQ"
     gene            complement(11641..11877)
                     /locus_tag="RH057_004740"
     CDS             complement(11641..11877)
                     /locus_tag="RH057_004740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HDV3130934.1"
                     /translation="MLTSITGHDSVLLRAEDPLIDMNYITSFTGMTDKWFYKLISEGH
                     FPKPIKLGRSSRWYKSEVEQWMQQRIEESRGAAA"
     gene            complement(11946..12518)
                     /locus_tag="RH057_004741"
     CDS             complement(11946..12518)
                     /locus_tag="RH057_004741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HDV3130935.1"
                     /translation="MNAIPYFDYSLAPFWPSYQNKVIGVLERALREQSGSRIRRILLR
                     LPCEYDNTFNSRTTWFGMDFIETVSALMNATPGRDLCWLLTRHPEKPEYHVVLCVRQE
                     YFDGPELDRLILDAWSNVLGFASPGEAKPYQKQITRDVVLDRRSPDCEALFKDLIWAF
                     SDFARDRRGVCDPEARCLAGNPWYPVAGQL"
     gene            complement(12776..13342)
                     /locus_tag="RH057_004742"
     CDS             complement(12776..13342)
                     /locus_tag="RH057_004742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HDV3130936.1"
                     /translation="MPKSYTPNWFFTALLDNHINQLVARYSRLRALRMDFFYRKDTPD
                     FLQPDHRWLELQLRMLLEQVEQFENIVGFFWVIEWTADHGFHAHAVFWLDRQRVKKIY
                     PFAERITECWRSITHNSGSAHRCTYQPHYTYNINIPVRHNDPESIDNIRGALHYLAKE
                     EQKDGLCAYGCSEVPERPAAGRPRKPHF"
BASE COUNT         3677 a         3467 c         3624 g         3339 t
ORIGIN      
        1 cagccgagaa gaatttcccc gcttattcgc accttcctta agtatcattg cagcaaagat
       61 gaaatcaatg atttatcaaa aatgattgaa aggtggttgt aaataatgtt acaatgtgtg
      121 agaagcagtc taaattcttc gtgaaatagt gatttttgaa gctaataaaa aacacacgtg
      181 gaatttagga aaaacttata tctgctgcta aatttaaccg tttgtcaaca cggtgcaaat
      241 caaacacact gattgcgtct gacgggcccg gacacctttt tgcttttaat tacggaactg
      301 atttcatgat gaaaaaatcg ttatgctgcg ctctgctgct gacagcctct ttctccacat
      361 ttgctgccgc aaaaacagaa caacagattg ccgatatcgt taatcgcacc atcaccccgt
      421 tgatgcagga gcaggctatt ccgggtatgg ccgttgccgt tatctaccag ggaaaaccct
      481 attatttcac ctggggtaaa gccgatatcg ccaataacca cccagtcacg cagcaaacgc
      541 tgtttgagct aggatcggtt agtaagacgt ttaacggcgt gttgggcggc gatgctatcg
      601 cccgcggcga aattaagctc agcgatccgg tcacgaaata ctggccagaa ctgacaggca
      661 aacagtggca gggtatccgc ctgctgcact tagccaccta tacggcaggc ggcctaccgc
      721 tgcagatccc cgatgacgtt agggataaag ccgcattact gcatttttat caaaactggc
      781 agccgcaatg gactccgggc gctaagcgac tttacgctaa ctccagcatt ggtctgtttg
      841 gcgcgctggc ggtgaaaccc tcaggaatga gttacgaaga ggcaatgacc agacgcgtcc
      901 tgcaaccatt aaaactggcg catacctgga ttacggttcc gcagaacgaa caaaaagatt
      961 atgcctgggg ctatcgcgaa gggaagcccg tacacgtttc tccgggacaa cttgacgccg
     1021 aagcctatgg cgtgaaatcc agcgttattg atatggcccg ctgggttcag gccaacatgg
     1081 atgccagcca cgttcaggag aaaacgctcc agcagggcat tgcgcttgcg cagtctcgct
     1141 actggcgtat tggcgatatg taccagggat taggctggga gatgctgaac tggccgctga
     1201 aagctgattc gatcatcaac ggcagcgaca gcaaagtggc attggcagcg cttcccgccg
     1261 ttgaggtaaa cccgcccgcc cccgcagtga aagcctcatg ggtgcataaa acgggctcca
     1321 ctggtggatt tggcagctac gtagccttcg ttccagaaaa aaaccttggc atcgtgatgc
     1381 tggcaaacaa aagctatcct aaccctgtcc gtgtcgaggc ggcctggcgc attcttgaaa
     1441 agctgcaata actgacgatg aggcccagga tattgggcct cctttctttc tctttttttc
     1501 ctgttgtcat ctacacttaa caaaaataca gcaaggaaaa tcccatgcgc attttgcccg
     1561 tcgttgctgc agttacggct gcattcctgg ttgtcgcgtg tagctccccg acaccgccga
     1621 aaggcgttac cgtggtaaat aactttgatg ccaaacgcta tctgggaacc tggtatgaaa
     1681 ttgcgcgctt cgaccatcgt ttcgagcgcg gattggataa agtgaccgca acatacagct
     1741 tgcgcgacga cggcggcatc aacgttatta acaagggcta taaccctgac agggagatgt
     1801 ggcagaaaac ggaagggaaa gcctatttca ccggcgaccc aagcagagcc gcgcttaagg
     1861 tttctttttt cggccccttc tatggcgggt ataacgtaat tgcactcgac cgggaatatc
     1921 gtcacgcgct ggtttgtggt ccggatcgcg actacctgtg gatcctttca cggaccccta
     1981 ctatttcaga tgaaatgaaa cagcaaatgt tagccatcgc gacccgggaa gggtttgaag
     2041 tgaataaact gatttgggtg aaacagcctg gcgcttagtg agtgctcagc ttcagaccaa
     2101 taatgccagc aacgatcagc ccaaggctca gcaaacgtgc cgggctggca gactcaccca
     2161 gcagcaaaat ccctgtaatg gccgccccaa cagcgccaat accggtccag accgcataag
     2221 cggttcctac aggcaacgtg cgcattgccc aagagagcat ggcgatactg acgatcatcg
     2281 ccgcaatagt gataatgctt ggcgtaagac gcgtaaaacc gtgggtgtat ttcaggccaa
     2341 tcgcccagac aacttcgagc aaacctgcaa ttaataaaac gatccaggac atatcaggct
     2401 ccagaacaat ggggccgtcc ccggtgaaag aagcgtttgc aggtcgtcct gcaaagctaa
     2461 tgtgtgaaat ggcatttttg cccggaagaa aatgaatttc aaccttttta ttcaccgcct
     2521 gctaaaagca agaattaagc ataattagcg gcgtagttcc cgcatttggc actcatcgga
     2581 gttctctatg atgaagggct ttctgaaacc agaaagtcga ttgcccctct tctgaatgcg
     2641 aaacgtttat gcttaaaatt ttagtgattg accggtgcca ctttacccgc acagggatag
     2701 aggccttgct taatcattct ggcaggttca gctcctcatt tctggtatca ggaatcaata
     2761 atctcctgtt agcaaaagag catattttgc aatggaaacc gcatctggtg atcgcggatt
     2821 tatacagttt tattagcgag acgcactcca gtccgccaat taaacctttt tttatgagtt
     2881 gtggcgtcat cccactaatt ttactgcaat cagcagacag acagcatgca cccatcgtgc
     2941 cctctcaatc cgtagcccat tcggtcttga ccaagcacac cacgctgaac acactctccc
     3001 acacaattca ggatttcctt atttgctcaa aaagggcact tccacagccc atgtttatgc
     3061 actccctccc gtaacctcca gcattgggtt cttgtaactc aaaacgaatg gagaatgcga
     3121 tgatcaattt caagcctaaa attccggcga tgcttggggc tctggcggtt cttaccgctg
     3181 gtgctgctca tgcagagctg ctggaataca cctttaaggc tccagatggt acgcaacggt
     3241 ctctgactcc gaatgccaac tatgccaacc caacgggcaa tatctcgttt gccttgagtg
     3301 ccggtatcga ccgaaaggta aagatttcgg tgcttcggtc ggatggaaca gtggtttcga
     3361 cagcgaccag ccaccttctt ggggctactg atcgcatcac agtgggtgga aaatcctact
     3421 atggtgcgga acttcaacta ccagctccag ttggtggggc atacactatc agggcggaaa
     3481 ttcttgcctc tgatggttct acagtacaga cggatgaata ccctcttact gtcgatgtga
     3541 cgccgccaac ctactcttcg cttgccccgg tttatagtaa ctatgggcag gttactagcg
     3601 gtgatgtttg gaaactgggt ctaggtggct ctgaggataa tgcctttttg ctgtcaggaa
     3661 tctctgatga gtcacccatc aaaggcgtca aagcaaagct gtatcgtcaa gacggttcac
     3721 tctacaaaga tgtatccgtg aactacgatg acgctaacgg acaagctaga cagtctttcc
     3781 aatctggctt tttcccagcc tctgacttgg atgaagtgtt tacacttcag tttgagataa
     3841 gcgatagcgc tggaaatagc tatctttcac ctcgtcaaaa agtgatgttt gatagcataa
     3901 ccaatgctcc gtctgcacct tttggtgttt atgacccttc aagtacaaac aacttaggcc
     3961 ctggattaac cggatttgtt gcttacactg agggcatgac agttaaaact aacccaatca
     4021 agttagcttg gcgtgttccg cgtgataact ggcatgagta tagagaaggt gggataaata
     4081 tgaccaatgc cttgggagag atgtccaagg taggagaaga tgccagctat gtttatctcg
     4141 tcacaaccgc accttatggc aataccgacg gcaattattg gcgttgggtg aacttcggcc
     4201 agtggggggg aggcggtatt gcttataacc tcacactctc tccatcagcg cctaaaagtc
     4261 ccagactttt gggtgtggac tacaattaca gtgatatagg atggtctagt ttttaccgct
     4321 actgggtgaa caattctgtt cttcctgtca ctgttagtag tatccgtgtc aaagttgaac
     4381 caaggcctta cgttcaaact gccgttcaca ggggaagctg tgaaattcct gttggtcaag
     4441 atagctgcgt gattgcaaat agcttcacaa tggctaaagg cacaacaggg tacgtccacg
     4501 ataatgcgac agttttcaat ccggataaat ctcttcgctc gaatcctctt tgggcagagg
     4561 ttaactggaa cgatcagcac taccctcagc taagtcagca gttcgatcaa aatagcaagg
     4621 tatttacctt attcgttaac cagccaggac gaggcgctta ctttgatagg ctgcgtctgc
     4681 gaagtgcatg gattgaagat agcaaaggca acaagctgtc tccaactggt gggttaatag
     4741 ccaacaactg ggagaactac acttaccagt gggatcttaa aactctccct gaggggcaat
     4801 acagtcttgt tgccgcagca gaggagatgc acgggccttt aacgcgacaa ccaatgttcc
     4861 agataacgtc tgatagaacg ccaccaacca tgacactcag tgtggcagat ggagcagcta
     4921 tccagactct agatgatgtt gttattacct tggctgatgc tatcgaccct tcacctaaat
     4981 tgacctctat tgcccttgta ggggggcctg ccaatgacaa ggtgcagttg tcttggcgtg
     5041 aggagtcgaa aggtcgattc cgccttgagt acccggtaat gttcccttct ttgaaggaag
     5101 gggaatctta cacgctgact gtttccggtg aggatgcaca aggcaatgcg gttcaaaagg
     5161 ctgttggctt cgagtacaaa cctcgtcagg taatgctggc ggatgggatg gatggcaagg
     5221 tcatggtccc cgctgtcact catgaatttg ttcatgcaga tggcaaacgg atcatcgaaa
     5281 ccaagccgct gacgctcagt gatggtgctg tcgtgacagg ttcatacgac gtgtttgcga
     5341 ccctacgttc tgatgcgaaa gtaccgctgg tggttaatgg ggtgcgtatc gagccaggcc
     5401 agacaatggg gatcatgagc caacatgatt tcggtgcgtc aggtggtcgt ttgagcattc
     5461 cggttaaacc tgctgttcct gatgtggtcg gctcttccag ccttcttgtc atgacctccg
     5521 cgccgaactc acccatcttg gttgtggaca tcaatacctg gaaagggacg gctaagctct
     5581 ctgctgaatc atggacaatt cgccaggtca ttgacccagt gaaaatctat gccctgccag
     5641 agtcgggtgt gccttgccgg ttcaccacga aagaggatgt ggctatggcc gcagacccaa
     5701 ttcgtgaccc ggtgtgtttg ctccagtggg acagaactcc agatgaggct gaacaaacta
     5761 cgcaggacaa caacgggatg aaagttgctg ggctggtggg gcaggctgtg agcattggtg
     5821 aacaacctgt cgaatacagc ctgtacctgt tcagtggtga cggttccaag gtaaaagttg
     5881 ggtctggctc tcagaacctg acggtaacta ctgcctatgg ctctgttggc tacaccccga
     5941 ttgatgacat tgctcaggtg aatcgcgtca ttgaagattt tgatgtgaac ttcaagcaga
     6001 gcaaggggcc agactgttct ataaccctca gcgcagaccg tgcgaaaaaa gaggccgcga
     6061 acaaggctgt tggtagtgca agccgtgcct gtctgttcga gtggcagcag atccccgatg
     6121 gattggtcca agacccgtta tcggaatcac cttcgctctc tggttcattg gcctccaacg
     6181 gcgttcatcc actggggtgg cgggtaagta ttttcacccg taacggcact agggtgacgt
     6241 tgaacgacga gactttcaat gtcgaagcgg ttgacccacc agctccgacc gttgaactag
     6301 cctccgacta caacttcaaa gacaacatct acttggtgcc gatgacaggt aactacctgg
     6361 gtgatgccat tatcaactct gaacgagctg atctggatat tgccatatcg cgcaactcgg
     6421 atgttcttga gtctgagacc ttcactccgg gatggggtgc caccaataag gtgtatcgcc
     6481 gcatcaatac cgatgagcga gcgttgtggg aggagaccac ctacaaggtg aacgcggcct
     6541 acaacaaggt gcctgatgtg aagactgagg ttgtctaccg ggctatctct gcaccttctg
     6601 acagtatccg tcctatcgtt gaagttaaag gtgataccgc gattgacacc caggcattgc
     6661 cggttagggt tctcatccgt gatcagtaca aacctgatgg tgactatgac gctaacacga
     6721 tgggggtgtg gaaagtacgc ctgatccagc aaaaggccta caacgagacg gttgcgctca
     6781 ctgattatgc ggaagcatcg aacggtgaag ctcagttctc agtagacctg tctggtgtgg
     6841 atacttcctc cgtccgtatc gctgctgagg ctgttctgga aagccctgtt gagggttaca
     6901 accgcacaga gttgtctatc agacctgcct tcttgacagt gcttcgcggt ggtgccatcg
     6961 gtgctggcgt ggaggctcgc aagttgtctg gtgaagctcc gttcactgct gtgttcaagc
     7021 tatctttgga cgaccgtcag gatctccggg ctaccggcca ggttgtgtgg gaaaccagta
     7081 aggacgacgg taaaacctgg gagcagttca tcccagaaga tcgatacaag tatcagcttg
     7141 tgaagacctt cgacaagggg gagtaccagg ttcgggccaa ggtggtgaac gtcaactcag
     7201 gtgcggaaaa gtacaccgaa gcggtcagtg ttgtcgctta cgacaaacct gatattgctg
     7261 ttatcggccc gaccacgttg tttgtcggaa gtgaaggcaa gtacacagcg aacctgacgt
     7321 tgaacgatga gccaatctcc ggtggcaatg ccattgttga gtggtctact gacggtggca
     7381 aaacctacgc gcagacaggg gatagcatca cgctttcgag cgatgaagaa acccgatacc
     7441 gcctgtgggc tcgtgtgcgc tctgccacgg caccggctga tgacggctat gcctatgaag
     7501 ttgcgaaaac ggcggttgac ttccgagcag tgaaggcacc tcgtccttac gtgacagggc
     7561 cgcgagttat tgagaccggt aagaagtatg tgttcaaagc cgaaaccagc ctgccttacc
     7621 gtgggatgga cgtgaagctg aacgggttct tcacgctgcc tgatggctcg attgtgcagg
     7681 gtgatactgc tgagtacgaa ccctctgaca ctgacctcaa tcaggctact gtagaaacga
     7741 agtacaccac ctggatcgaa ggataccgag atcagggtgc agaggcttcg catagcctac
     7801 gctcccgcgt ttggcagtat gtatggccga gcttcggaat gcaggttagg aagaacgcag
     7861 acgtggctcc tgcgacgatc accgcgtcag tgcggccaat tgccttcaac ggcaagctgg
     7921 aagagccgac ctacgagtgg gagttgccgg aaggcgctgt gattcaggat cagcggcagg
     7981 atattgtccg gtcctttgtg atcaatgagc cgggtgatta caacatcaag gtcactgtcc
     8041 gtgatgctcg cggccatgag accgtgatcg agcaaccgct caagatcggc caggcagcgc
     8101 cctatgccat tgacctgcaa tactctggct cgaacaaata cgagcgtgag cctctggatg
     8161 tgctgttgcg accgtacatt tctggcggcc acccacgcga ccgtatttcg actcgtgttt
     8221 attcggtaga tggtacaccg ttggaaagca gtggttacta cggcagagca actctgggcg
     8281 ctggtgagca cagcatcaag ctgaaaataa cctcagaaat ggggcatgaa gctgagggcg
     8341 aggtgaacat caatgttgca gagaacaagt tgcctgcatg tagcctgagc tcacgagaga
     8401 ccgttgggtc gtggatcgtc tatgcgaact gcgaagatac cgatggccgc atgaagtcct
     8461 acgaatggac cattgccggt gagttgcaga gcatcagctc tgatcgagtg actatcagca
     8521 agggcaccta tgaaacgatg ccgaccatct ctctggttgg ggtcgatgac tctgggggca
     8581 aatccgaagc tgttaccatg aactaaggtt cattcctctc aaagcccggt tcagaccggg
     8641 cttttttttg tatcgcgctg gaattgtcca aattgggcta ttacagaggc gagtctattt
     8701 ccatcccccg gatacactgc ccagcatcca gtaatccaat cttaggggat agacatgcgg
     8761 acaaaattac ttggggcgct gatggtgttc gggattatta ccggcacggc tcatgcgtca
     8821 tcgaaattgg aaatcaccga tcccagagcg gcgaagatag aggacattgt agagctaccc
     8881 atcaaagggg ttcgagccgt ccaaagtgat gggcagatca tgttcctctc tgaaaacgga
     8941 cgatttgtta tttcaggaca aatctacgac ctgtggagca agaagcccct caacacgatg
     9001 tcccaaatga gggatgtagc ggagcgtatc cacttcaaga gcatgggcat ggatgtggac
     9061 acgctgaaca ctgtgtcgat ggggcgtggt gacaaagagg tggtggtctt tgttgatcct
     9121 aggtgcgcgg tttgccatca gctcatgggt gatgccaaat cgctggtgga tgattacacc
     9181 tttaaattta tcgtgattcc agctctgggt gctgagtcca accgcctggc aaaaaacttg
     9241 tactgcgcga aagacaaaac ccacgcgctt gatgcgctga tgaacaacac cctgggttcc
     9301 cttccttcaa aagaaacctg cgaccccggc caatacgacc aaacgctgct gacagctcat
     9361 ttcattggga ttgagggcgt tccgttcgtt gttgctccgg atggtcgtgt cagcaaagga
     9421 cgtccgaaga acctgaaatc atggttggag agtgctgaat gatcgtaacc atcaaaaaga
     9481 aactcgaaga aacgttgatc ccggagcact tgcgagctgc cgggattatt cctgtcctgg
     9541 cctatgacga agacgatcat gtcttcctta tggatgatca cagtgcaggc tttggtttca
     9601 tgtgtgagcc cctgtgtggt gccgatgaaa aagttcagga gcgaatgaac ggtttcctga
     9661 atcaggagtt cccgtcgaag actacgctcc agtttgtcct gttccgctcc ccggacatca
     9721 atcaggagat gtaccggatg atgggtttgc gtgatggctt ccgtcacgag ctgctgacat
     9781 ctgttatcaa ggaacggatt aacttcctcc agcaccacac gacagatcgc atatttgcca
     9841 agaccaacaa aggtatctac gacaatggct tgatccaaga cctcaagctg ttcgttacgt
     9901 gcaaagtccc catcaagaac aataacccga ctgaaagcga actccagcag ctcgcacagc
     9961 ttcgcacgaa ggtcgaatca tcgcttcaaa ccgttggtct gcgtcctcgc acaatgacgg
    10021 cggtgaacta catccggatc atgagcacca tcctgaattg ggggccagat gcttcatggc
    10081 gacatgactc tgtggattgg gagatggata agcccatctg cgagcaaatc ttcgattacg
    10141 gcaccgacgt ggaagtcagc ttacctgctg attgcttcac acccgggctc ctcgcactgc
    10201 gcgcgggtta ttgtttcgtt ttcagccccg gggtcgccaa ggctctggat ggggtactac
    10261 ttccattgac agcatgaggt aacaatactg atggtaacgc tgaccattaa ccgtaaacca
    10321 cagggcattt tcagtaaatc accggcaacg ccgctgcagg ataaaatgaa cactgcacac
    10381 aacgtgaagg ccagggaacg gataccacag ttgaacgata aacagagccc tgctctgctg
    10441 aaaaaacacc gtggcggcat atgacaaaac gccagcgtaa aaaccgtaag cgtattaatc
    10501 gtcttgtgga acactggcct gagttattca accgggaaaa accccaaccg cgtaaagttg
    10561 agatacctga cgacctgata caggatatcg ccatcagaga actggcgttt agcgcaggtg
    10621 cattacgcgc ggcagtagcg tcttatgtgc agtctccgcg ctattaccgt gcgttaatgg
    10681 ctggcggcgc ccgttacgac ctgaaggggc agccgtgcgg ggaagtgact ccacaggaac
    10741 agaaagaggc agagacccgg ctaatggtgc tgaatgaccg acaaaaagcc agaaaacctc
    10801 gttgactaca caactgcgct gattcactga gctgtaatca atgtacagcg ctgtaccgga
    10861 cgatgggtaa tggcggtcac aaccggtaac cccagagaca atgcgacggt ttttaacgct
    10921 acatactgac cggatgtatc accccgggca acccgaaaac acctttcaac ctggcattgt
    10981 ccgccagcgc ttatcacctt ccaggtctgt tgcaataaat ccacccgttg cgaacattct
    11041 tcatcaaccg tggcactggc ccgcggatgg caaatgctcg tctggctgag cagcaacagg
    11101 catcgtattg ttgctcctcc atgttgttcc cgcaaccagc gtaataccac cggcgaggat
    11161 ggacaggcag tgtgattacg ctcagtaata cgttcgtgca cctgtcggta aaaggaagta
    11221 cagaatgcat gaatctgttg cccgttgatg tttccttctg ccgaatagag cgtgaagtga
    11281 aatgccagca gatttggcca gtggtccacc gcctgctgaa caaaacgccg gatgtctccc
    11341 ggctctgaaa ttaaggcttc ggttatttgc acgaaatgtt ccttttcaaa ccctgttgaa
    11401 ttttgttaag ccggtgcaga cacacgggaa ggtgcgcatc aacaccggtg taatgccagt
    11461 tataaacaat gtgaatggaa tgacgaatca gtcaggaaag agctacaacc tgctggctga
    11521 ccggagaggg tcagcaggaa aatgaacggt aatgtcgcca gcaccaggga cagggctgat
    11581 ttccgcaata ccagcgccgg cattttgcac attgccagcg taccggcatc acaacacgtt
    11641 tcatgctgct gctcctcgtg attcctcaat tcgctgctgc atccactgct ccacttcact
    11701 tttgtaccag cggctgctgc ggcccagctt gatgggtttc gggaaatggc cttcactgat
    11761 cagcttgtaa aaccatttat cggtcatgcc ggtgaaactg gtgatgtagt tcatgtcgat
    11821 caggggatcc tcggcacgca gcaatacact gtcgtggcct gttattgagg tcaacatagt
    11881 cagaatatcc tctgtgagat ttatgaaggt tgggcaaatg cgggtgaatc tgctgaactg
    11941 agctttcaga gctggcctgc tacgggatac cagggattgc cggcaaggca acgggcttcc
    12001 ggatcgcaca ctccacggcg atcgcgggcg aaatcactga acgcccagat aaggtcctta
    12061 aacagggctt cgcagtccgg tgaccggcga tccagtacca catcccgggt gatctgcttc
    12121 tggtacggct ttgcttcacc tggtgacgcg aaacccagca cgttactcca ggcatccaga
    12181 atcaaccgat ccagttcggg gccgtcgaaa tactcctgtc tgacgcataa caccacgtgg
    12241 tattccggct tttcaggatg acgggtcagg agccagcaaa ggtcacgtcc gggtgttgca
    12301 ttcatcagcg cactgacggt ttcgataaaa tccataccaa accaggtcgt tctgctgttg
    12361 aaggtattgt catattcaca cggcagacga agcaggatcc gccgtatccg tgagccggac
    12421 tgctcacgca gcgcacgctc aaggacgccg atgactttgt tctgataaga tggccagaag
    12481 ggggccaggc tatagtcgaa ataagggata gcattcataa gatatgctcc gttatgcgca
    12541 gcccacaatg cgtcctgccc gtgaataagc tgacgtgtta tggtttgcgg atggggttga
    12601 tggaataagt gaagaaccgg ggcagaacgg gaagatcagg tcaccgacaa gggacagaac
    12661 accggatgtg gttcagagtg aatgatctcc gttgacttca gttcacatca ttaagcaagg
    12721 cggtaaggtt ttaccgacgg aatgtttcca ggacaggaag ctcaggattt aagcttcaga
    12781 agtgaggctt acgaggacgc cctgcagcag gtcgttcagg aacttcactg caaccgtatg
    12841 cgcacagtcc gtctttttgc tcttctttcg ccagataatg cagggcaccg cgaatattat
    12901 cgatgctttc aggatcattg tggcgcacag gaatgttgat gttgtatgta taatgcggct
    12961 gatatgtaca gcggtgtgcc gaaccgctgt tatgcgtaat agaccgccag cattccgtaa
    13021 tccgctccgc aaagggatat atttttttaa ccctctgacg atcgagccag aaaaccgcat
    13081 gcgcatgaaa accatgatca gccgtccatt caatcaccca gaagaaacca acgatatttt
    13141 caaattgttc cacctgctcc agcagcatac gcaactgcaa ttcaagccag cgatgatcag
    13201 gttgtaagaa atcaggcgta tctttcctgt agaagaaatc catgcgtaag gcccgcaggc
    13261 gggaatagcg tgcgaccaac tgattgatgt gattgtcaag taaagcggta aaaaaccagt
    13321 ttggtgtgta agatttaggc atgctgatga ctcctgaaag ttaatgacgt tttaagtgct
    13381 gtaaacgaat taatgctttc gctcataatc agatctaaaa cggtaataac ttacagacgg
    13441 tatttatcag gcatgctgta acagaatgga tacgtcggtt attaacgaca cgtagcgttg
    13501 tgcagaaata aatggattat ctgatggtat tagctaaaga gatgagataa acgaggagcg
    13561 aataaaatac agaatcaata aacatcacaa aatactgcaa aacacgcgat aaaaacccac
    13621 gaccagaagt tcaagaaaca tattacgacc aataacatca taaactacaa acttgctctc
    13681 agtggttcta aaaaaaacgt atacaaggcc aacacaataa acttatacgc cctcatccaa
    13741 tctgttctta aggacatgaa cgttaaccgc acacattacc agagtaaaaa ataccactgg
    13801 catgattttt accctgcatc ttaaacaata caacccgaaa aaaacataat caggaattca
    13861 ctctgttaat ttcattgttt atcctgcact gttaaacatg acgaaatcat ggacacctta
    13921 tcaaagagta aacattcagt taatcagtga tgacagaagt attgatattt accagaatgg
    13981 ttaaaacata tcagataaac aatcattgtt aagcaaatac agctcatgat ggataaaatt
    14041 aaaataaatt attattttac tgaaaacagg aacagatgga tatcagcaat agcgtagtag
    14101 agaataa
//
LOCUS       DAPHDW010000422        10590 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.guided.154, whole genome shotgun sequence.
ACCESSION   DAPHDW010000422 DAPHDW010000000
VERSION     DAPHDW010000422.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 10590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10590
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.guided.154"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            <1..851
                     /gene="motA"
                     /locus_tag="RH057_004743"
     CDS             <1..851
                     /gene="motA"
                     /locus_tag="RH057_004743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001169515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HDV3130937.1"
                     /translation="GLLVVLGCVFGGYFEAGGQLVAIWQPAEMIIILGAGFGAMIIGN
                     PKHVLKEIAHQIKGVISKKQLGPEFQRQLLMCLYELLEMVQNGGLRMLDQHIEQPEES
                     TIFQKYPLVLTQKRLVTFIADNFRLMAMGKIDAHELEGILDQELDTAEESLLTPSRSL
                     QRTAEAMPGFGICAAVLGIIITMQSIDGSIALIGLKVAAALVGTFLGVFICYCLMDPL
                     ANAMEQQARAEHSLLECVRTVLVAQAGGKPTLLAVDAGRKLLHLASKPTFANLDAWVN
                     AMLEQE"
     gene            854..1777
                     /gene="lafU"
                     /locus_tag="RH057_004744"
     CDS             854..1777
                     /gene="lafU"
                     /locus_tag="RH057_004744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HDV3130938.1"
                     /translation="MRKTGNRRERGAKTTIVRRQIKKNHAGHHGGAWKVAFADFTLAM
                     MALFMTLWIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSHPPKPATVAVPEE
                     TEKKARDVNEKTALLKKKSATELGELATSITTIARDAHMEANLEMEIVPQGLRVLIKD
                     DQNRNMFERGSAQIMPFFKTLLVELAPVFDSLDNKIIITGHTDAMAYKNNIYNNWNLS
                     GDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDSKNPQSAGNRRIEIMVLTKSASDT
                     LYQYFGQHGDKVVQPLVQKLDKQQVLSQRTR"
     gene            1848..2903
                     /gene="dinB"
                     /locus_tag="RH057_004745"
     CDS             1848..2903
                     /gene="dinB"
                     /locus_tag="RH057_004745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HDV3130939.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFSELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLAKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            2900..3352
                     /locus_tag="RH057_004746"
     CDS             2900..3352
                     /locus_tag="RH057_004746"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HDV3130940.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTASQHYSPQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPLIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGEWFINFYMRYKQQH"
     gene            3598..4737
                     /locus_tag="RH057_004747"
     CDS             3598..4737
                     /locus_tag="RH057_004747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HDV3130941.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSVGRFYPALVGNDIGCGMALWQTDILTRKYNADKFEKRLSD
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGVGQSILQRHIASFSHHGLPQGSDDALRYIAEHDDALAFACINRQM
                     IALRIMQQVKATGSPVLDVAHNFVSACQIGDQQGWLHRKGATPDDNGLVIIPGSRGDY
                     SWIVQPVANEKTLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQL
                     IFEEAPQAYKSAESVVQCLVQAGLIIPVARLRPVLTLKNSGGKKG"
     gene            4734..5348
                     /locus_tag="RH057_004748"
     CDS             4734..5348
                     /locus_tag="RH057_004748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
                     /protein_id="HDV3130942.1"
                     /translation="MILLQLSSAQGPEECCLAVKKALDRLIKEAARQDVAVTVLETET
                     GRYSDTLRSALVSLDGDNAWALSESWCGTIQWICPSPYRPHHGRKNWFLGIGRFTADE
                     QEQSDAIRYETLRSSGPGGQHVNKTDSAVRATHLASGISVKVQSERSQHANKRLARLL
                     IAWKLEQQQQENSAVLKSQRRMFHHQIERGNPRRTFTGMAFIEG"
     gene            complement(5405..6862)
                     /gene="pepD"
                     /locus_tag="RH057_004749"
     CDS             complement(5405..6862)
                     /gene="pepD"
                     /locus_tag="RH057_004749"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HDV3130943.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRKPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDVL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            7123..7581
                     /gene="gpt"
                     /locus_tag="RH057_004750"
     CDS             7123..7581
                     /gene="gpt"
                     /locus_tag="RH057_004750"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HDV3130944.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            7673..8917
                     /gene="frsA"
                     /locus_tag="RH057_004751"
     CDS             7673..8917
                     /gene="frsA"
                     /locus_tag="RH057_004751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HDV3130945.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACAEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDDA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            8975..9376
                     /gene="crl"
                     /locus_tag="RH057_004752"
     CDS             8975..9376
                     /gene="crl"
                     /locus_tag="RH057_004752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HDV3130946.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPADDFRDEPVKLTA"
     gene            complement(9415..10470)
                     /gene="phoE"
                     /locus_tag="RH057_004753"
     CDS             complement(9415..10470)
                     /gene="phoE"
                     /locus_tag="RH057_004753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HDV3130947.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNDSKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDIVAVGMTYQF"
BASE COUNT         2597 a         2568 c         2795 g         2630 t
ORIGIN      
        1 taggtttact ggtggtgctc ggctgcgtat ttggcggtta ttttgaagcg ggcgggcaat
       61 tagtggctat ctggcaaccg gcggaaatga tcattatttt gggtgccggt ttcggggcga
      121 tgattattgg taacccgaaa catgtgctga aagagattgc ccatcaaatt aaaggcgtga
      181 tcagtaaaaa acagctcggg ccggagtttc agcgtcagct attaatgtgc ctgtatgagt
      241 tgctcgaaat ggtgcaaaac ggtggtctgc gcatgctgga tcaacatatt gagcaaccgg
      301 aagagagcac cattttccag aaatatccgc tggtgttaac ccagaagcgg ctggtgacct
      361 ttattgccga taactttcgc ctgatggcga tgggtaaaat cgacgcccat gaactggaag
      421 gcattctcga tcaggaactg gacactgcgg aagagtcgct gctcacgccg tcgcgctcgc
      481 tgcaacgtac cgccgaagcg atgccgggat ttggtatttg tgcagcagtg ctggggatca
      541 tcatcaccat gcagtcgatt gacggctcta ttgccctgat cggcctgaaa gtggcggcag
      601 cgctggtggg gacgttttta ggcgtcttta tctgctactg cctgatggac ccgctcgcca
      661 acgccatgga acaacaggcg cgggcagaac attctttgct ggaatgcgtg cgcactgtgc
      721 tggtggcgca ggcaggcggc aaaccgacgc tgctggcggt ggatgccggg cgtaagctgc
      781 tgcatctggc gtccaaaccg accttcgcca acctcgacgc gtgggtgaat gcgatgctgg
      841 agcaagaata agcatgagaa agacgggcaa ccgccgcgag cgtggggcaa aaaccaccat
      901 cgtccggcgg cagatcaaaa agaatcatgc cgggcatcat ggcggggcgt ggaaggtggc
      961 gttcgccgac tttactttgg caatgatggc gctgtttatg accctgtgga ttgtcaacag
     1021 cgtcagtaaa tccgagcgtg aaagcattat cgccgcgctg cacgggcagt cgatttttaa
     1081 cggtggtggg ctgtcgccgt taaataaaat cagcccgtct catccgccaa aacctgccac
     1141 cgtggcggtg ccggaagaaa cggagaaaaa agcgcgtgat gtgaatgaga aaacggcgct
     1201 gctgaagaag aaatccgcca ccgagcttgg cgagctggca accagtatca ccaccattgc
     1261 ccgtgatgcg catatggaag cgaatctgga gatggagatt gttccccagg gattacgcgt
     1321 gctgattaaa gacgaccaga atcgcaatat gttcgaacgc ggcagcgcgc agattatgcc
     1381 gttctttaaa acgctgctgg tggagctcgc gccagttttc gactcgctcg ataacaaaat
     1441 tattattacc gggcataccg atgcgatggc ttacaaaaac aatatctaca acaactggaa
     1501 cctttcgggt gaccgcgcgc tttcggctcg tcgggtgctg gaagaggccg gaatgccgga
     1561 agataaagtg atgcaggtaa gcgcaatggc ggaccagatg ctgctggatt ccaaaaatcc
     1621 gcaaagcgcg ggcaaccggc gcattgagat tatggtgctg accaaaagtg cgtccgatac
     1681 gctgtatcaa tactttggtc agcatgggga taaagtggtg cagccgctgg tgcaaaagct
     1741 ggataagcag caggtgcttt cgcagcgaac gcgttaaatg ctgaatcttt acgcatttct
     1801 caaaccctga aatcactgta tactttacca gtgttgagag gtgagtaatg cgtaaaatca
     1861 ttcatgtgga tatggactgc tttttcgcag cggtggagat gcgcgacaat cccgccctgc
     1921 gcgatatccc tattgctatt ggcggcagcc gcgaacgtcg gggggtgatc agcaccgcca
     1981 attatcccgc gcgtaaattt ggcgtacgta gcgctatgcc gacagggatg gcgctcaaat
     2041 tatgcccgca tctcaccttg cttccggggc gctttgacgc ctacaaagaa gcctcaaatc
     2101 atatccgtga aatcttctcg cgctacacct cgcgtattga accattgtca ctggatgagg
     2161 cttatctcga cgtcaccgat agcgtccatt gccacggttc tgcgaccctc atcgcccagg
     2221 aaatccgcca gacgattttc agcgagctgc aactgacagc gtctgcgggc gtggcaccag
     2281 taaagtttct cgccaaaatc gcctccgaca tgaataaacc caacggtcag tttgtgatta
     2341 cgccggcaga agttccggca tttttacaaa ccttaccgct ggcaaaaatc cccggcgtcg
     2401 gcaaagtctc ggcggcaaaa ctggaagcga tggggctgcg gacctgcggt gatgtacaaa
     2461 agtgtgatct ggtgatactg cttaaacgct ttggcaaatt tggccgcatt ttgtgggagc
     2521 gtagtcaggg gattgacgag cgcgacgtta acagcgaacg gttgcgaaaa tccgtcggcg
     2581 tggaacgcac gatggcggaa gatatccacc actggtctga atgtgaagcg attatcgagc
     2641 ggctgtatcc ggaacttgaa cgccgtctgg caaaggtaaa acctgattta ctgattgccc
     2701 gccagggggt gaaattaaag ttcgacgatt ttcagcaaac cactcaggag cacgtctggc
     2761 cgcggctgaa taaagctgac ttaatcgcca ccgcgcgtaa aacctgggat gaacgccgcg
     2821 gcgggcgcgg tgtgcgactg gtggggctgc atgtgacgtt gcttgacccg caaatggaaa
     2881 gacaactggt gctgggatta tgaataacat acaaataaga gactatcagc ctggcgattt
     2941 tcagcaacta tgcgctattt tcattagagc ggttacgatg actgccagtc agcattattc
     3001 accacaacaa attgccgcct gggcgcagat tgacgaatct cgctggaagg agaaactcgc
     3061 gaaatcacaa gtgcgggttg cagtcattaa tgcaaaactg gtcggcttta ttacctgcgt
     3121 tgaacactat atcgatatgt tatttgttga ccctgaatac acccgccgtg gggttgccag
     3181 cgctttgtta aaacctttga ttaagtctga atccgaactt acggtggacg caagcataac
     3241 cgcaaaaccc ttttttgaac gttatggttt tcagacagtt aagcagcaac gcgttgaatg
     3301 tcggggagag tggtttatta atttttatat gcgttataaa cagcaacatt aaatccagct
     3361 tgcaatgaaa ataacgcaag cctagtatgt gcgcgttttc ttattccaca aactgcaagg
     3421 aggtaaatca tgacaaatcc tttatcaatg actctttgca gacctttcca ggattaattc
     3481 tttttttctt gccctggatt cgtctgccat ttcctgattt tttaattgtt actctcaata
     3541 attcaagtcg caacacatgc aacttgaagt atgacgagtc tatttacgga atggtatatg
     3601 ggcaattata ttcgtccctt atccgatgcg gtatttacca tcgcatctga tgacctgtgg
     3661 atcgagagtt tagcgatcca acaattacac accacggcaa atttacccaa catgcagcgc
     3721 gtagtgggga tgccagattt acatcccgga cgcggctatc cgattggcgc agcgttcttc
     3781 tctgttggtc gtttttatcc ggcactggtc ggcaatgata tcggctgcgg tatggcgcta
     3841 tggcaaacag atattctcac tcgcaaatac aacgccgata agtttgaaaa gcgattatca
     3901 gatctggatg acgttgctga agaaagctgg ctggaggaaa acctgccgtc agcatttgca
     3961 cagcatccgt ggcgcagctc gcttggctct atcggtggcg gcaatcattt cgctgaactg
     4021 caacaagttg atcaaatcat caacgctgaa ctgtttgcac tggcaggtct ggatgcacaa
     4081 catctgcaac tgttggttca tagcggctcg cggggggtgg ggcagtctat tttacagcgg
     4141 catattgcct cgttctcgca tcatggtttg cctcaaggca gtgacgacgc gctacggtat
     4201 attgcggaac atgacgatgc gctggcgttt gcgtgtatta atcgccagat gatcgctttg
     4261 cgcataatgc aacaagttaa ggccacggga agtccggttc tggatgtggc gcataacttt
     4321 gttagcgcgt gtcaaatcgg cgatcaacag ggctggttgc atcgtaaagg tgccacgccg
     4381 gatgacaacg gtctggtgat tattcccggt tcacgtggtg attactcctg gattgtacag
     4441 cctgttgcga acgaaaaaac gctccactcg ctggcgcatg gggcagggcg taaatggggg
     4501 cgcaccgagt gtaaagggcg tctggcagcg aaatacacgg cgacgcaact ctcgcgtact
     4561 gaacttggca gtcgggtaat ttgtcgcgat aaacaactca tctttgaaga agcgccacaa
     4621 gcctataaat cggctgaaag cgtggtgcaa tgtctggtgc aggccggttt aattattcct
     4681 gtcgcgcggc tgcgtccggt gctaacgctc aaaaacagtg gagggaaaaa aggatgatct
     4741 tgctacaact ctcctcagcg caggggccgg aagaatgttg tctcgcagtg aaaaaggcgc
     4801 tggacaggct aattaaagaa gctgcccgac aagacgtcgc ggtaacggtg ttggaaacag
     4861 aaacgggccg ctactctgac acgctgcgtt cagctctggt ttctctggat ggcgataacg
     4921 catgggcatt aagcgaaagc tggtgcggca ctattcagtg gatttgtccg agtccgtatc
     4981 ggcctcatca tgggcgcaaa aactggtttc tgggcattgg gcgttttacc gctgatgagc
     5041 aggaacaatc ggatgcaatc cgttatgaga cgttgcgttc gtcggggccg ggcggtcaac
     5101 atgtcaataa aaccgactcg gcggtacgcg ccacgcattt ggcatccggt attagcgtga
     5161 aggttcagtc agagcgtagt cagcatgcta acaagcggct ggcacgattg ctgattgcct
     5221 ggaagctgga gcagcagcaa caggaaaata gcgcggtgct gaaatcgcag cggcgaatgt
     5281 tccatcacca gattgaacgt ggcaacccga gacggacatt tacagggatg gcttttatcg
     5341 aaggataatg aaggatgaaa ctgccggaag gcgattaaac gccatccggc agcaaatcaa
     5401 ataattactt cgccggaatt tctttcagca gttcagtcag cagtgtccag taatgaccta
     5461 cgctttcgat gtgaacttgc tcatccggag agtgtggacc ggtgatagtt ggcccgatag
     5521 aaaccatgtc catttccgga tacggttttt tgaacagacc acattccagg cccgcgtgga
     5581 taatctggat gttcggtgtt ttgttgaaca gacgctgata ggtttcacgt accagatgca
     5641 tcaccggaga attagcgtcc ggctgccagc caggatatgc gcctttcgct tcggttttcg
     5701 cgccagctag tttacccagc gaatccagca tgctcaccac gtagtcttta ccgctgtcga
     5761 tcagtgaacg gatcaggcag tgaatttcta cgttattgtc agtcatggtc accacaccga
     5821 cgttcaggga ggtttcgacc acaccttttg ccacgtcgga attgcggatc acaccgttcg
     5881 gggtggcgtt cagcagacga ataaaggtat cgcgagattt cgcaatcagg gcagctttat
     5941 cgttcgctac agagtccagc aacaaggcca gatttttctc tttctctgcc agctcgtttt
     6001 tcaggatctc ctgataggta ttcaccagag atttcaggac gtcgacttta tcagctgcga
     6061 cagcaatggt cgcaaaggct tcacgcggga tggcgttacg cagtgtgccg ccgttgaaat
     6121 cgataaggcg cagatccagt tcttccgcat gacccgccag gaagcgcacc agtagtttgt
     6181 tggcattacc caggccaacg tggatttcac cgccggagtg accgcctttc agacctttta
     6241 aggttaactt gaaggtttca aaaccagctg gaaccgcttc acgatctaaa tgcaggttgg
     6301 aggtgaagtc gatacccccc gcacaaccca tgtagatttc accttcttct tcggagtcgg
     6361 tgttaatcag aatatcagcc tgcaaccagt tgctctgtaa gccgaacgca ccgtccatac
     6421 cggcttcttc ggtcatggtc agcagcactt ccagcgggcc gtgaaccacg ttttcgtcag
     6481 ccagaaccgc cagcgcagac gccataccaa tgccgttatc tgcacccagc gtggtgccgc
     6541 gcgctttaac ccattcgcca tcaatataag gctggatagg atctttcgtg aagtcatgca
     6601 cggtgtcgtt atttttctgc ggcaccatat cgaggtgggc ctgtaagacg accggtttac
     6661 gattttccat acctgcggta gcaggtttac gaatcaggat attacctacc tgatcgcgtt
     6721 cgacatggaa acctttctct tttgcccaac caacaatgta ttcagcgagt tgctcttcat
     6781 gataggacgg gtgaggaata gaacagattt tggcaaaaat atcccacagc ggctgtggag
     6841 ataattgaga cagttcagac acgttaagtc tccttgtcga tcacccgcaa aacagtattg
     6901 caggtcacag ggttagcaga aaatgttgtc aacacaagac aggcttgcga gatatgtttg
     6961 agaataccac tttatcccgc gtcagggaga ggcagtgcgt aaaaagacgc ggactcatgt
     7021 gaaatactgg tttttagtgc gccagatctc tataatctcg cgcaacctat tttcccctcg
     7081 aacacttttt aagccgtaga taaacaggct gggacacttc acatgagcga aaaatacatc
     7141 gtcacctggg acatgttgca gatccatgca cgtaaactcg caagccgact gatgccttct
     7201 gaacaatgga aaggcattat tgccgtaagc cgtggcggtc tggtaccggg tgcgttactg
     7261 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     7321 aaccagcgcg agcttaaagt gctaaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     7381 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     7441 gcacattttg tgactatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     7501 gttgatatcc cgcaagatac ctggatcgaa cagccgtggg atatgggcgt cgtattcgtc
     7561 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     7621 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     7681 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     7741 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggctcttg atggtaaaac
     7801 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     7861 acgtgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgctgaac gtaccgatga
     7921 tgatttatac gataccgtga ttggctaccg tggtggcaac tggatttatg agtgggccac
     7981 ccaggcgatg gtgtggcaac aaaaagcctg tgcggaagaa gatccgcaac tcagtggtcg
     8041 acactggctg catgcggcta cgttgtacaa cattgccgcc tatcctcatc tgaaaggaga
     8101 tgacctggcc gagcaagcgc aggctttatc aaaccgcgcc tatgaagagg ccgcccagcg
     8161 tctgccgggc acgatgcggc agatggagtt taccgtaccc ggcggtgcgc ccatcaccgg
     8221 ctttttgcat atgccgaaag gcgatggccc gttcccgaca gtattaatgt gtggtggtct
     8281 ggatgcgatg cagacggact attacagcct gtatgaacgt tattttgcgc cgcgcggcat
     8341 tgcgatgctg actattgata tgccgtcggt gggcttttct tcaaaatgga agctcaccca
     8401 ggactccagc ctgttgcatc agcacgtctt aaaggcgctg cctaacgtac cgtgggtgga
     8461 tcacactcgc gtcgcggcct ttggtttccg tttcggcgct aacgttgccg tgcgtctggc
     8521 ataccttgaa tcgccgcgtc tgaaagcggt tgcctgtctt ggtccggtag ttcataccct
     8581 gttgagtgat tttaagtgcc agcaacaggt gccggaaatg tatcttgacg ttctggcgag
     8641 tcgtttgggg atgcatgatg cttccgatga cgcgttgcgc gtggagctga atcgctattc
     8701 attaaaagtg caaggattgc tgggacgtcg ctgcccaacg ccaatgttat caggctactg
     8761 gaagaacgat ccgttcagcc cggaagagga ctcacgctta atcacctcat catctgctga
     8821 cggtaaatta ttagagatcc catttaaccc ggtgtatcgg aattttgaca aaggtcttca
     8881 ggaaatcacc ggctggatcg aaaaacgctt gtgttaaaaa tttgctaaat tttgccaatt
     8941 tggtaaaaca gttgcatcac aacaggagat agcaatgacg ttaccgagtg gacacccgaa
     9001 gagcagattg atcaaaaaat ttaccgcact aggcccgtat attcgtgaag gtaagtgcga
     9061 agataatcga ttctttttcg attgtctggc tgtatgcgtc aacgtgaaac cggcaccgga
     9121 agtgcgtgaa ttctggggct ggtggatgga gctggaagcg caggaatccc gttttaccta
     9181 cagttaccag tttggtctgt tcgataaagc aggcgactgg aagagtgttc cggtaaaaga
     9241 cactgaagtg gttgaacgac tggagcacac cctgcgtgag tttcacgaga agctgcgtga
     9301 actgctgacg acgctgaatc tgaagctgga accggcggat gattttcgtg acgagccggt
     9361 gaagttaacg gcgtgagtga aatgtgccgg atgcatcaca tccggcaata atcattaaaa
     9421 ctgataggtc atgccaaccg cgacaatatc atcattatta atattcaatt tgttatcgct
     9481 atccagttgg ttgattttat aatcaacaaa cgctgacata tttttgttga aataatatgt
     9541 agccccgaca tcgatataat tgaccagatc ttcatcaccg ataccttcaa tatctttccc
     9601 tttcgataag acataaccca gcgatggacg cagaccaaag tcaaactggt attgagcgac
     9661 cgcttcaaag ttctgtgtct tattggcaaa gccgccagtt attggcgtca ttttgcgtgt
     9721 ttcagaataa aaagttgcca gataaatatt attggcatcg tatttcagac ctgttgccca
     9781 tgcttctgca cgcttgcctg tgccacggct ttgcaggttc tgctcgttgg tgcgatctga
     9841 gttggtatag gccccactaa tggcgaaatc gctgccgcca aagtcatatg tcaatgacgt
     9901 gccgaagcca tcgccgtttt gctttttaac gtcgcggttt tcgtttttcc cttgatattg
     9961 cagggttaag ttcaggccat cgataacgcc gaagaagtcg gtgttccgat acgtcgccag
    10021 accgctggcg cgtttggtca taaagttgtc ggtctgcgcc gaggagtcgc caccaaattc
    10081 cgggaacata tcggtccagg cttccacgtc atacaacgcg cccaggttac ggccatagtc
    10141 gaaagaaccc aaatctttat acttcaatcc ggcaaaagcg agacgcgttt tttgctgtgc
    10201 agtatcactc tccgctttat ttccggcaaa ctccgcttcc caacggccat agccagtcag
    10261 ttgatcgtta atttgtgttt cgcctttaaa accaaaacgg atataactct ggtcgccatc
    10321 tttactgtcg ttatcactca tataatgcat ggctttaact ttgccataga catccagttt
    10381 attaccgtct ttgttatata tttctgcggc ctgtacggat gcagatgcca caatgcccat
    10441 caccactaat gccagagtgc tctttttcat tttcattcct gattttaatt aacgcgcgaa
    10501 tattcagcgg gagagccccg ttgaaaacag gaaagttttt aacctgagat tgttaaagat
    10561 atattacaga ttaataatat tcttaaaatg
//
LOCUS       DAPHDW010000423         5099 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.guided.219, whole genome shotgun sequence.
ACCESSION   DAPHDW010000423 DAPHDW010000000
VERSION     DAPHDW010000423.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.guided.219"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            28..843
                     /locus_tag="RH057_004754"
     CDS             28..843
                     /locus_tag="RH057_004754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(3')-I family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HDV3130948.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGEFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(997..1872)
                     /locus_tag="RH057_004755"
     CDS             complement(997..1872)
                     /locus_tag="RH057_004755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
                     /protein_id="HDV3130949.1"
                     /translation="MRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTS
                     DGCTTVAGVNAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTF
                     AVNEKGIVNTVNNIRAVYRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTL
                     GQLGDARLFSVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQ
                     WRETQPPFDAAAAKTILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIF
                     DINRDDAKFHGEVRG"
     gene            complement(1942..4827)
                     /locus_tag="RH057_004756"
     CDS             complement(1942..4827)
                     /locus_tag="RH057_004756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDV3130950.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRR
                     AHNRFGFALQLCVLRYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHE
                     HLAELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATS
                     TIERLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGS
                     NSSSANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLA
                     ILAVCVSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIG
                     GALVDAQDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRY
                     APRMLRLLDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDAR
                     LWEIAVLFHLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLAD
                     RQARLDMRLRELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPT
                     RITDLLLEVDAATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHT
                     FWELIRIGRWHVEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEA
                     MNLVNAKYGNTPGLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQ
                     FTDTGGFTDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAM
                     IERNWPDILRIAATIAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWI
                     LDAELQRRAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIF
                     WNTMKLGEVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
     gene            4953..>5099
                     /locus_tag="RH057_004757"
     CDS             4953..>5099
                     /locus_tag="RH057_004757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3130951.1"
                     /translation="MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDGTVAKLAM
                     RQPFV"
BASE COUNT         1070 a         1448 c         1493 g         1088 t
ORIGIN      
        1 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg
       61 aggccgcgat taaattccaa cctggatgct gatttatatg ggtataaatg ggctcgcgat
      121 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
      181 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
      241 ctaaactggc tgacggcatt tatgcctctt ccgaccatca agcattttat ccgtactcct
      301 gatgatgcat ggttactcac cactgcgctc cccgggaaaa cagcattcca ggtattagaa
      361 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagcgttcct gcgccggttg
      421 cattcgattc ctgttagtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
      481 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
      541 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat
      601 tcagtcgtca ctcatggtga attctcactt gataacctta tttttgacga ggggaaatta
      661 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
      721 ctatggaact gcctcggtga attttctcct tcattacaga aacggctttt tcaaaaatat
      781 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
      841 tgatagttag tctttgtaga gacacaaacc tgaaattccg gacaccgcgc ttcaggacat
      901 ctccctggac gccgatatct ggcagtatcc ggacggtact atcgaacgcc gggccaacgg
      961 tactcccctg ccctttatga ctgacgaccg gctatttcac ccacgcactt ctccatgaaa
     1021 tttagcatcg tcgcggttaa tgtcgaaaat ttgccacctg acgtggttgg catcgagggt
     1081 gaaaactgcc gtggcgaggg tgttctcctc atccggatcg tcggcagcca gccggtaaat
     1141 tggcagttct gcgtcatggg tatctgagag aatggttttt gccgccgcgg cgtcaaacgg
     1201 aggttgcgtt tcccgccact gcacgaggcg taactgccgc gacgctgaac tgtcggtaac
     1261 gatctgctca acgccagcca gtgccgcatg caccagatgg ttggcatgcc cggttaccgt
     1321 cgtcaggggt agaacggaac agccttgacc ggtggcctca acgctgaaca gccgcgcatc
     1381 gcccagctgc ccgagcgtat ggtgaaacgc cccggatcgc ggctggccgg taagtatagt
     1441 gatggcctcg tcgagggtgt tcgcattcag cgaggcgcga gccaggatct gacgcggtaa
     1501 tccctgcgga cgatacactg cacggatgtt attcaccgtg ttaacgatac ctttttcatt
     1561 taccgcaaag gtgtggccgc aaatcgaccc cggataggca aaactcgtaa agctcagacc
     1621 gcgactcgga gtaatggtag ccagcaagca ttcatcccgt aactgaggaa aaccgtcttc
     1681 attatgcgcg ataatccgct cgcctgcggc gtttaccccg gccaccgtcg tacagccatc
     1741 cgacgttgag cgcaccagat cgccacggca gttccaggca aagacctgat caacagtggc
     1801 ctccagtccc tgcgccagcc cctccagctc ctgccagatg tgcggataat gccgctgcac
     1861 ctcggcgcgc attaaccggg tctcagggga tgcctgcagg gtcatcacgg ggtcgtttgc
     1921 gggagggggc ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat
     1981 gtgttcccat ccgagcggcg aaacatgggc caagagatcg ggcgatagca gctttccatc
     2041 gcgtttctgg tttgcaacga cctcgccgag cttcatggtg ttccagaaga tgatgatggc
     2101 ggcgagcaga ttcatgccgg cgatgcggta atgctggcct tcggcggaac ggtcgcggat
     2161 ttcaccgcgg cggtggaagc tgattgcccg cttcagcgca tgatgagctt cgcctttgtt
     2221 gagcccgatc tgggcacgcc gttggagttc ggcatccaga atccagtcga tcatgaacag
     2281 ggtgcgctcg acgcgaccga cttcccgcag ggctgtcgcg agctcgttct gccgcggata
     2341 ggaggcgagt ttccgcagaa tctggcttgg cgcgacggtc ccggcagcaa tggtggcggc
     2401 gatgcgcagg atgtcgggcc aattgcgctc gatcatggct tggttgacct ttccgccgat
     2461 caacgctcgc aggtgcgccg gggcggccga cggattgaac gcgtagagcc gtttggatgg
     2521 caggtcgcgg atgcgcggag cgaaccggta gccgagaatg gcacatgcgg caaagacgtg
     2581 atcggtgaag ccgcccgtgt cggtgaactg ctcgcggata tggcgtccag catcgttcat
     2641 cagcaggcca tcgaggatgt aaggcgcttc gcttgccgtt gcaggaatca cctgggttgc
     2701 gaacggcgca tattggtcgg agacgtggct ataggctttc aggcccgggg tattgccata
     2761 tttcgcgttg accaggttca tggcctcacc ttgctctgta gcgacgaaga actgtccgtc
     2821 gctcgaagcc gacgtgccca tgccccagaa ccgggccatg ggtaacgctg cctgtgcctc
     2881 gaccaccatg gccagcgccc ggtcataggc ttcgccctcg acatgccacc gtccaatgcg
     2941 gatcaattcc cagaaggtgt gggtgtttgt cgcatccgcc attttgcgca agccgaggtt
     3001 gatcccttcc gccaagataa cgttcattag cccgatccgg tcagcgcagg gtgctcctgt
     3061 gcgcagatgg gtgaacgctt cggtgaagcc ggtcgccgca tccacctcca gcaggagatc
     3121 ggtgatgcgc gtgggcggga tctgcttgta gagatcgagc accagatctt cggcgcctgt
     3181 cggcgcggcg gcttcgagtt tctcgatatg cagaacgccg ttttcaatcg acccgcccgg
     3241 gatcgtgcct gcgcgagcgg cacggccaag ctcgcgcaac cgcatgtcga ggcgagcttg
     3301 ccggtctgcc agccattcct ccggccgcaa tggcacagcg agacgaccgc cttccgcgat
     3361 ggattgtgcc ggaacgagtg cgtgtttcag atcgccatag cgccgggacc tagtaagcca
     3421 gacatctccg gagcggaacg catcgcgcag atggaacagc accgcgatct cccataggcg
     3481 agcgtcgcca gccctctggg cccgaaggtg gcgatgccat ttcgagctgg gccgcaagaa
     3541 gctggtcatc gcggcatcgt tcaaaccggt acgaagggcc gtcaccgctt ccagaagcgg
     3601 cagtgcaacg ggcgcagctc gcagatcgag caggcgcaac atgcgtggag cgtatcggcg
     3661 gaagcggtga taaccgtcga gcacatgatt gagcggatcg tcggccatgg tggcggtcag
     3721 cctggttgcc attgcaacaa gggtttttaa gccgtcccac cctgacccac tcgcgatgac
     3781 atcgcccagc ggctggccat catcctgtgc atcgaccagg gcgcccccga tctcggcgaa
     3841 ggatttcagg gtgtcacgca ccacccccgc ttcgtctgcg acctttgcat ggcaaatacg
     3901 ctccgaagca cggtagagac ggccgacgat ccggtcgtgg gtttcgacca ctgcgtcggc
     3961 caacatcgcc tgccattccg agacgcaaac agccaagatc gcaagccgcc tgtcctccgg
     4021 gagatcgcgc atgccgtcgg cataataccg ttcaccctgc ctgcgcagac gagtcacccg
     4081 atgggcagga acgccggcaa gcagatcctc ggggagatcg atgcgttgca gatattcgag
     4141 ccggtcgagc agccggttgg ccgacgaaga gttcgagcca ggctcgaact ggcgcagcca
     4201 cacaaaacgg gtcacccgat catcagccgt ctcctcgagc aatgccagca actgttctcg
     4261 gatcgacata ggcagccgac tggcgatcct cgtctcgatg cgtcgctcgg catcgacgag
     4321 agccgcggca caaagccgct cgatcgtgga tgtcgcggga aggacagtgc gggtgcgtcg
     4381 gcactcggct acgaagcgac gggcgatatc ctcgttcgac accgccatct cggcttctcg
     4441 gaacaaccat tccttcagct cgctcgcacc acgtccggag aaggtgcgga agccgtagag
     4501 cccccgtaac tcggcaagat gctcgtgccg tgtttcctcg cgggcagcat agtctacgag
     4561 atcgtcggca cccaggccaa gctgcgctcc gataaattcg atgacctctg cagggatcag
     4621 ttcgcctgga gccagcaccc ggccgggata gcgcaggaca cacaattgca gggcgaagcc
     4681 gaacctgttg tgagcgcgcc gacgcagcct gatatgccca aggtcttcat cactcagcgt
     4741 atagtgcttg agcaaatccg tctgtgaagt cggcaagcgc aacagcgcgt ctttctgccg
     4801 atcggttaga gtgacgcgac gcggcataca tgttcctttt tcaaaatctg atagcgttca
     4861 agacgctttg tttatgaagc tggttgagat acacttccag aggtcaatgc aatcgtggcc
     4921 gaagcgccgc ctcaaaccaa cgtttgtgat acatgctgat cggatatgcc cgcgtctcca
     4981 aagccgatgg ctcgcagtct ctcgacctgc agcacgacgc cttgcgcgcc gcaggtgtcg
     5041 aacgggacaa tatctatgat ggcactgttg caaagttagc gatgaggcag ccttttgtc
//
LOCUS       DAPHDW010000424         5099 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.guided.220, whole genome shotgun sequence.
ACCESSION   DAPHDW010000424 DAPHDW010000000
VERSION     DAPHDW010000424.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 5099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5099
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.guided.220"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            28..843
                     /locus_tag="RH057_004758"
     CDS             28..843
                     /locus_tag="RH057_004758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032492145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3')-Ia"
                     /protein_id="HDV3130952.1"
                     /translation="MSHIQRETSCSRPRLNSNLDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTAFMPLPTIKHFIRTPDDAWLLTTALP
                     GKTAFQVLEEYPDSGENIVDALAAFLRRLHSIPVSNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     gene            complement(997..1872)
                     /locus_tag="RH057_004759"
     CDS             complement(997..1872)
                     /locus_tag="RH057_004759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001201005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA--6-aminopenicillanic acid
                     acyl-transferase"
                     /protein_id="HDV3130953.1"
                     /translation="MRAEVQRHYPHIWQELEGLAQGLEATVDQVFAWNCRGDLVRSTS
                     DGCTTVAGVNAAGERIIAHNEDGFPQLRDECLLATITPSRGLSFTSFAYPGSICGHTF
                     AVNEKGIVNTVNNIRAVYRPQGLPRQILARASLNANTLDEAITILTGQPRSGAFHHTL
                     GQLGDARLFSVEATGQGCSVLPLTTVTGHANHLVHAALAGVEQIVTDSSASRQLRLVQ
                     WRETQPPFDAAAAKTILSDTHDAELPIYRLAADDPDEENTLATAVFTLDANHVRWQIF
                     DINRDDAKFHGEVRG"
     gene            complement(1942..4827)
                     /locus_tag="RH057_004760"
     CDS             complement(1942..4827)
                     /locus_tag="RH057_004760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
                     /protein_id="HDV3130954.1"
                     /translation="MPRRVTLTDRQKDALLRLPTSQTDLLKHYTLSDEDLGHIRLRRR
                     AHNRFGFALQLCVLRYPGRVLAPGELIPAEVIEFIGAQLGLGADDLVDYAAREETRHE
                     HLAELRGLYGFRTFSGRGASELKEWLFREAEMAVSNEDIARRFVAECRRTRTVLPATS
                     TIERLCAAALVDAERRIETRIASRLPMSIREQLLALLEETADDRVTRFVWLRQFEPGS
                     NSSSANRLLDRLEYLQRIDLPEDLLAGVPAHRVTRLRRQGERYYADGMRDLPEDRRLA
                     ILAVCVSEWQAMLADAVVETHDRIVGRLYRASERICHAKVADEAGVVRDTLKSFAEIG
                     GALVDAQDDGQPLGDVIASGSGWDGLKTLVAMATRLTATMADDPLNHVLDGYHRFRRY
                     APRMLRLLDLRAAPVALPLLEAVTALRTGLNDAAMTSFLRPSSKWHRHLRAQRAGDAR
                     LWEIAVLFHLRDAFRSGDVWLTRSRRYGDLKHALVPAQSIAEGGRLAVPLRPEEWLAD
                     RQARLDMRLRELGRAARAGTIPGGSIENGVLHIEKLEAAAPTGAEDLVLDLYKQIPPT
                     RITDLLLEVDAATGFTEAFTHLRTGAPCADRIGLMNVILAEGINLGLRKMADATNTHT
                     FWELIRIGRWHVEGEAYDRALAMVVEAQAALPMARFWGMGTSASSDGQFFVATEQGEA
                     MNLVNAKYGNTPGLKAYSHVSDQYAPFATQVIPATASEAPYILDGLLMNDAGRHIREQ
                     FTDTGGFTDHVFAACAILGYRFAPRIRDLPSKRLYAFNPSAAPAHLRALIGGKVNQAM
                     IERNWPDILRIAATIAAGTVAPSQILRKLASYPRQNELATALREVGRVERTLFMIDWI
                     LDAELQRRAQIGLNKGEAHHALKRAISFHRRGEIRDRSAEGQHYRIAGMNLLAAIIIF
                     WNTMKLGEVVANQKRDGKLLSPDLLAHVSPLGWEHINLTGEYRWPKP"
     gene            4953..>5099
                     /locus_tag="RH057_004761"
     CDS             4953..>5099
                     /locus_tag="RH057_004761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HDV3130955.1"
                     /translation="MLIGYARVSKADGSQSLDLQHDALRAAGVERDNIYDGTVAKLAM
                     RQPFV"
BASE COUNT         1069 a         1448 c         1493 g         1089 t
ORIGIN      
        1 tacataaaca gtaatacaag gggtgttatg agccatattc aacgggaaac gtcttgctcg
       61 aggccgcgat taaattccaa cctggatgct gatttatatg ggtataaatg ggctcgcgat
      121 aatgtcgggc aatcaggtgc gacaatctat cgattgtatg ggaagcccga tgcgccagag
      181 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
      241 ctaaactggc tgacggcatt tatgcctctt ccgaccatca agcattttat ccgtactcct
      301 gatgatgcat ggttactcac cactgcgctc cccgggaaaa cagcattcca ggtattagaa
      361 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagcgttcct gcgccggttg
      421 cattcgattc ctgttagtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
      481 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
      541 ggctggcctg ttgaacaagt ctggaaagaa atgcataagc ttttgccatt ctcaccggat
      601 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
      661 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
      721 ctatggaact gcctcggtga attttctcct tcattacaga aacggctttt tcaaaaatat
      781 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
      841 tgatagttag tctttgtaga gacacaaacc tgaaattccg gacaccgcgc ttcaggacat
      901 ctccctggac gccgatatct ggcagtatcc ggacggtact atcgaacgcc gggccaacgg
      961 tactcccctg ccctttatga ctgacgaccg gctatttcac ccacgcactt ctccatgaaa
     1021 tttagcatcg tcgcggttaa tgtcgaaaat ttgccacctg acgtggttgg catcgagggt
     1081 gaaaactgcc gtggcgaggg tgttctcctc atccggatcg tcggcagcca gccggtaaat
     1141 tggcagttct gcgtcatggg tatctgagag aatggttttt gccgccgcgg cgtcaaacgg
     1201 aggttgcgtt tcccgccact gcacgaggcg taactgccgc gacgctgaac tgtcggtaac
     1261 gatctgctca acgccagcca gtgccgcatg caccagatgg ttggcatgcc cggttaccgt
     1321 cgtcaggggt agaacggaac agccttgacc ggtggcctca acgctgaaca gccgcgcatc
     1381 gcccagctgc ccgagcgtat ggtgaaacgc cccggatcgc ggctggccgg taagtatagt
     1441 gatggcctcg tcgagggtgt tcgcattcag cgaggcgcga gccaggatct gacgcggtaa
     1501 tccctgcgga cgatacactg cacggatgtt attcaccgtg ttaacgatac ctttttcatt
     1561 taccgcaaag gtgtggccgc aaatcgaccc cggataggca aaactcgtaa agctcagacc
     1621 gcgactcgga gtaatggtag ccagcaagca ttcatcccgt aactgaggaa aaccgtcttc
     1681 attatgcgcg ataatccgct cgcctgcggc gtttaccccg gccaccgtcg tacagccatc
     1741 cgacgttgag cgcaccagat cgccacggca gttccaggca aagacctgat caacagtggc
     1801 ctccagtccc tgcgccagcc cctccagctc ctgccagatg tgcggataat gccgctgcac
     1861 ctcggcgcgc attaaccggg tctcagggga tgcctgcagg gtcatcacgg ggtcgtttgc
     1921 gggagggggc ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat
     1981 gtgttcccat ccgagcggcg aaacatgggc caagagatcg ggcgatagca gctttccatc
     2041 gcgtttctgg tttgcaacga cctcgccgag cttcatggtg ttccagaaga tgatgatggc
     2101 ggcgagcaga ttcatgccgg cgatgcggta atgctggcct tcggcggaac ggtcgcggat
     2161 ttcaccgcgg cggtggaagc tgattgcccg cttcagcgca tgatgagctt cgcctttgtt
     2221 gagcccgatc tgggcacgcc gttggagttc ggcatccaga atccagtcga tcatgaacag
     2281 ggtgcgctcg acgcgaccga cttcccgcag ggctgtcgcg agctcgttct gccgcggata
     2341 ggaggcgagt ttccgcagaa tctggcttgg cgcgacggtc ccggcagcaa tggtggcggc
     2401 gatgcgcagg atgtcgggcc aattgcgctc gatcatggct tggttgacct ttccgccgat
     2461 caacgctcgc aggtgcgccg gggcggccga cggattgaac gcgtagagcc gtttggatgg
     2521 caggtcgcgg atgcgcggag cgaaccggta gccgagaatg gcacatgcgg caaagacgtg
     2581 atcggtgaag ccgcccgtgt cggtgaactg ctcgcggata tggcgtccag catcgttcat
     2641 cagcaggcca tcgaggatgt aaggcgcttc gcttgccgtt gcaggaatca cctgggttgc
     2701 gaacggcgca tattggtcgg agacgtggct ataggctttc aggcccgggg tattgccata
     2761 tttcgcgttg accaggttca tggcctcacc ttgctctgta gcgacgaaga actgtccgtc
     2821 gctcgaagcc gacgtgccca tgccccagaa ccgggccatg ggtaacgctg cctgtgcctc
     2881 gaccaccatg gccagcgccc ggtcataggc ttcgccctcg acatgccacc gtccaatgcg
     2941 gatcaattcc cagaaggtgt gggtgtttgt cgcatccgcc attttgcgca agccgaggtt
     3001 gatcccttcc gccaagataa cgttcattag cccgatccgg tcagcgcagg gtgctcctgt
     3061 gcgcagatgg gtgaacgctt cggtgaagcc ggtcgccgca tccacctcca gcaggagatc
     3121 ggtgatgcgc gtgggcggga tctgcttgta gagatcgagc accagatctt cggcgcctgt
     3181 cggcgcggcg gcttcgagtt tctcgatatg cagaacgccg ttttcaatcg acccgcccgg
     3241 gatcgtgcct gcgcgagcgg cacggccaag ctcgcgcaac cgcatgtcga ggcgagcttg
     3301 ccggtctgcc agccattcct ccggccgcaa tggcacagcg agacgaccgc cttccgcgat
     3361 ggattgtgcc ggaacgagtg cgtgtttcag atcgccatag cgccgggacc tagtaagcca
     3421 gacatctccg gagcggaacg catcgcgcag atggaacagc accgcgatct cccataggcg
     3481 agcgtcgcca gccctctggg cccgaaggtg gcgatgccat ttcgagctgg gccgcaagaa
     3541 gctggtcatc gcggcatcgt tcaaaccggt acgaagggcc gtcaccgctt ccagaagcgg
     3601 cagtgcaacg ggcgcagctc gcagatcgag caggcgcaac atgcgtggag cgtatcggcg
     3661 gaagcggtga taaccgtcga gcacatgatt gagcggatcg tcggccatgg tggcggtcag
     3721 cctggttgcc attgcaacaa gggtttttaa gccgtcccac cctgacccac tcgcgatgac
     3781 atcgcccagc ggctggccat catcctgtgc atcgaccagg gcgcccccga tctcggcgaa
     3841 ggatttcagg gtgtcacgca ccacccccgc ttcgtctgcg acctttgcat ggcaaatacg
     3901 ctccgaagca cggtagagac ggccgacgat ccggtcgtgg gtttcgacca ctgcgtcggc
     3961 caacatcgcc tgccattccg agacgcaaac agccaagatc gcaagccgcc tgtcctccgg
     4021 gagatcgcgc atgccgtcgg cataataccg ttcaccctgc ctgcgcagac gagtcacccg
     4081 atgggcagga acgccggcaa gcagatcctc ggggagatcg atgcgttgca gatattcgag
     4141 ccggtcgagc agccggttgg ccgacgaaga gttcgagcca ggctcgaact ggcgcagcca
     4201 cacaaaacgg gtcacccgat catcagccgt ctcctcgagc aatgccagca actgttctcg
     4261 gatcgacata ggcagccgac tggcgatcct cgtctcgatg cgtcgctcgg catcgacgag
     4321 agccgcggca caaagccgct cgatcgtgga tgtcgcggga aggacagtgc gggtgcgtcg
     4381 gcactcggct acgaagcgac gggcgatatc ctcgttcgac accgccatct cggcttctcg
     4441 gaacaaccat tccttcagct cgctcgcacc acgtccggag aaggtgcgga agccgtagag
     4501 cccccgtaac tcggcaagat gctcgtgccg tgtttcctcg cgggcagcat agtctacgag
     4561 atcgtcggca cccaggccaa gctgcgctcc gataaattcg atgacctctg cagggatcag
     4621 ttcgcctgga gccagcaccc ggccgggata gcgcaggaca cacaattgca gggcgaagcc
     4681 gaacctgttg tgagcgcgcc gacgcagcct gatatgccca aggtcttcat cactcagcgt
     4741 atagtgcttg agcaaatccg tctgtgaagt cggcaagcgc aacagcgcgt ctttctgccg
     4801 atcggttaga gtgacgcgac gcggcataca tgttcctttt tcaaaatctg atagcgttca
     4861 agacgctttg tttatgaagc tggttgagat acacttccag aggtcaatgc aatcgtggcc
     4921 gaagcgccgc ctcaaaccaa cgtttgtgat acatgctgat cggatatgcc cgcgtctcca
     4981 aagccgatgg ctcgcagtct ctcgacctgc agcacgacgc cttgcgcgcc gcaggtgtcg
     5041 aacgggacaa tatctatgat ggcactgttg caaagttagc gatgaggcag ccttttgtc
//
LOCUS       DAPHDW010000425         2609 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.guided.261, whole genome shotgun sequence.
ACCESSION   DAPHDW010000425 DAPHDW010000000
VERSION     DAPHDW010000425.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2609
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.guided.261"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            8..631
                     /locus_tag="RH057_004762"
     CDS             8..631
                     /locus_tag="RH057_004762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HDV3130956.1"
                     /translation="MSDISRVKILSALMDGRAWTATELSSVANISASTASSHLSKLLD
                     CQLITVVAQGKHRYFRLAGKDIAELMESMMGISLNHGVHAKVSTPVHLRKARTCYDHL
                     AGEVAVKIYDSLCQQQWITENGSMITLSGIQYFHEMGIDVPSKHSRKICCACLDWSER
                     RFHLGGYVGAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(609..1232)
                     /gene="tetR(B)"
                     /locus_tag="RH057_004763"
     CDS             complement(609..1232)
                     /gene="tetR(B)"
                     /locus_tag="RH057_004763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HDV3130957.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            1314..2519
                     /gene="tet(B)"
                     /locus_tag="RH057_004764"
     CDS             1314..2519
                     /gene="tet(B)"
                     /locus_tag="RH057_004764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HDV3130958.1"
                     /translation="MNSSTKIALVITLFDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVTFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
BASE COUNT          666 a          504 c          580 g          859 t
ORIGIN      
        1 ggcggctatg tctgacattt cacgagttaa aatactcagt gctttgatgg atgggcgagc
       61 ttggacggcc actgagctaa gttctgtggc gaatatatca gcttcaacgg cgagcagtca
      121 tttatctaaa ttattagatt gccagctaat cacagtagta gctcaaggca agcatcgtta
      181 ttttcggcta gcaggaaaag atattgctga attgatggaa agtatgatgg ggatctcctt
      241 aaaccatggc gtacatgcca aagtttccac gccagtgcat ttacgaaaag cacgtacttg
      301 ctatgatcat ttagctggcg aagttgccgt taagatctat gattcccttt gtcaacagca
      361 atggatcact gaaaatggtt caatgatcac attaagtggt attcaatatt ttcatgaaat
      421 gggaattgac gttccttcca aacattcacg taaaatctgt tgtgcgtgtt tagattggag
      481 tgaacgccgt ttccatttag gtgggtacgt tggagccgca ttattttcgc tttatgaatc
      541 taaagggtgg ttaactcgac atcttggtta ccgtgaagtt accatcacgg aaaaaggtta
      601 tgctgctttt aagacccact ttcacattta agttgttttt ctaatccgca tatgatcaat
      661 tcaaggccga ataagaaggc tggctctgca ccttggtgat caaataattc gatagcttgt
      721 cgtaataatg gcggcatact atcagtagta ggtgtttccc tttcttcttt agcgacttga
      781 tgctcttgat cttccaatac gcaacctaaa gtaaaatgcc ccacagcgct gagtgcatat
      841 aatgcattct ctagtgaaaa accttgttgg cataaaaagg ctaattgatt ttcgagagtt
      901 tcatactgtt tttctgtagg ccgtgtacct aaatgtactt ttgctccatc gcgatgactt
      961 agtaaagcac atctaaaact tttagcgtta ttacgtaaaa aatcttgcca gctttcccct
     1021 tctaaagggc aaaagtgagt atggtgccta tctaacatct caatggctaa ggcgtcgagc
     1081 aaagcccgct tattttttac atgccaatac aatgtaggct gctctacacc tagcttctgg
     1141 gcgagtttac gggttgttaa accttcgatt ccgacctcat taagcagctc taatgcgctg
     1201 ttaatcactt tacttttatc taatctagac atcattaatt cctaattttt gttgacactc
     1261 tatcattgat agagttattt taccactccc tatcagtgat agagaaaagt gaaatgaata
     1321 gttcgacaaa gatcgcattg gtaattacgt tattcgatgc catggggatt ggccttatca
     1381 tgccagtctt gccaacgtta ttacgtgaat ttattgcttc ggaagatatc gctaaccact
     1441 ttggcgtatt gcttgcactt tatgcgttaa tgcaggttat ctttgctcct tggcttggaa
     1501 aaatgtctga ccgatttggt cggcgcccag tgctgttgtt gtcattaata ggcgcatcgc
     1561 tggattactt attgctggct ttttcaagtg cgctttggat gctgtattta ggccgtttgc
     1621 tttcagggat cacaggagct actggggctg tcgcggcatc ggtcattgcc gataccacct
     1681 cagcttctca acgcgtgaag tggttcggtt ggttaggggc aagttttggg cttggtttaa
     1741 tagcggggcc tattattggt ggttttgcag gagagatttc accgcatagt ccctttttta
     1801 tcgctgcgtt gctaaatatt gtcactttcc ttgtggttat gttttggttc cgtgaaacca
     1861 aaaatacacg tgataataca gataccgaag taggggttga gacgcaatcg aattcggtat
     1921 acatcacttt atttaaaacg atgcccattt tgttgattat ttatttttca gcgcaattga
     1981 taggccaaat tcccgcaacg gtgtgggtgc tatttaccga aaatcgtttt ggatggaata
     2041 gcatgatggt tggcttttca ttagcgggtc ttggtctttt acactcagta ttccaagcct
     2101 ttgtggcagg aagaatagcc actaaatggg gcgaaaaaac ggcagtactg ctcggattta
     2161 ttgcagatag tagtgcattt gcctttttag cgtttatatc tgaaggttgg ttagttttcc
     2221 ctgttttaat tttattggct ggtggtggga tcgctttacc tgcattacag ggagtgatgt
     2281 ctatccaaac aaagagtcat cagcaaggtg ctttacaggg attattggtg agccttacca
     2341 atgcaaccgg tgttattggc ccattactgt ttgctgttat ttataatcat tcactaccaa
     2401 tttgggatgg ctggatttgg attattggtt tagcgtttta ctgtattatt atcctgctat
     2461 cgatgacctt catgttaacc cctcaagctc aggggagtaa acaggagaca agtgcttagt
     2521 tatttcgtca ccaaatgatg ttattccgcg aaatataatg accctcttga taacccaaga
     2581 gggcattttt tacgataaag aagatttag
//
LOCUS       DAPHDW010000426         2218 bp    DNA     linear   BCT 07-SEP-2023
DEFINITION  TPA_asm: Escherichia coli isolate ARS-CC16537
            SAMN37211189-rid19350763.guided.276, whole genome shotgun sequence.
ACCESSION   DAPHDW010000426 DAPHDW010000000
VERSION     DAPHDW010000426.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN37211189
            Sequence Read Archive: SRR25824663
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Escherichia coli
  ORGANISM  Escherichia coli
            Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Escherichia.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-SEP-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-SEP-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001875387.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001875387.1
            Genome Coverage       :: 62x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 09/07/2023 02:32:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,766
            CDSs (total)                      :: 4,690
            Genes (coding)                    :: 4,535
            CDSs (with protein)               :: 4,535
            Genes (RNA)                       :: 76
            rRNAs                             :: 3, 2, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 3 (5S)
            partial rRNAs                     :: 2, 2 (16S, 23S)
            tRNAs                             :: 61
            ncRNAs                            :: 8
            Pseudo Genes (total)              :: 155
            CDSs (without protein)            :: 155
            Pseudo Genes (ambiguous residues) :: 0 of 155
            Pseudo Genes (frameshifted)       :: 61 of 155
            Pseudo Genes (incomplete)         :: 94 of 155
            Pseudo Genes (internal stop)      :: 31 of 155
            Pseudo Genes (multiple problems)  :: 26 of 155
            CRISPR Arrays                     :: 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Escherichia coli"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN37211189-rid19350763.guided.276"
                     /isolate="ARS-CC16537"
                     /isolation_source="dairy calf feces"
                     /db_xref="taxon:562"
                     /country="USA"
                     /collection_date="2017"
                     /collected_by="USDA ARS"
     gene            233..1036
                     /locus_tag="RH057_004765"
                     /note="aph(3'')-Ib"
     CDS             233..1036
                     /locus_tag="RH057_004765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HDV3130959.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            1036..1872
                     /locus_tag="RH057_004766"
     CDS             1036..1872
                     /locus_tag="RH057_004766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HDV3130960.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
BASE COUNT          499 a          565 c          607 g          547 t
ORIGIN      
        1 gtgtatctac cttaacttaa tgattttgat aaaaatcatt aggggattca tcagcactcg
       61 gctggaaatc gccggtggca tttgagaaaa aagccgctta aaatgagaga tagaccggaa
      121 cacaaccggt gcaagaccat ggaaagcatg ttctcggact tacgtagcaa ctcgtttctt
      181 ttcgcaggtt gagccacctc cgcgcttcat cagaaaactg aaggaacctc cattgaatcg
      241 aactaatatt ttttttggtg aatcgcattc tgactggttg cctgtcagag gcggagaatc
      301 tggtgatttt gtttttcgac gtggtgacgg gcatgccttc gcgaaaatcg cacctgcttc
      361 ccgccgcggt gagctcgctg gagagcgtga ccgcctcatt tggctcaaag gtcgaggtgt
      421 ggcttgcccc gaggtgatca actggcagga ggaacaggag ggtgcatgct tggtgataac
      481 ggcaattccg ggagtaccgg cggctgatct gtctggagcg gatttgctca aagcgtggcc
      541 gtcaatgggg cagcaacttg gcgctgttca cagcctatcg gttgatcaat gtccgtttga
      601 gcgcaggctg tcgcgaatgt tcggacgcgc cgttgatgtg gtgtcccgca atgccgtcaa
      661 tcccgacttc ttaccggacg aggacaagag tacgccgcag ctcgatcttt tggctcgtgt
      721 cgaacgagag ctaccggtgc ggctcgacca agagcgcacc gatatggttg tttgccatgg
      781 tgatccctgc atgccgaact tcatggtgga ccctaaaact cttcaatgca cgggtctgat
      841 cgaccttggg cggctcggaa cagcagatcg ctatgccgat ttggcactca tgattgctaa
      901 cgccgaagag aactgggcag cgccagatga agcagagcgc gccttcgctg tcctattcaa
      961 tgtattgggg atcgaagccc ccgaccgcga acgccttgcc ttctatctgc gattggaccc
     1021 tctgacttgg ggttgatgtt catgccgcct gtttttcctg ctcattggca cgtttcgcaa
     1081 cctgttctca ttgcggacac cttttccagc ctcgtttgga aagtttcatt gccagacggg
     1141 actcctgcaa tcgtcaaggg attgaaacct atagaagaca ttgctgatga actgcgcggg
     1201 gccgactatc tggtatggcg caatgggagg ggagcagtcc ggttgctcgg tcgtgagaac
     1261 aatctgatgt tgctcgaata tgccggggag cgaatgctct ctcacatcgt tgccgagcac
     1321 ggcgactacc aggcgaccga aattgcagcg gaactaatgg cgaagctgta tgccgcatct
     1381 gaggaacccc tgccttctgc ccttctcccg atccgggatc gctttgcagc tttgtttcag
     1441 cgggcgcgcg atgatcaaaa cgcaggttgt caaactgact acgtccacgc ggcgattata
     1501 gccgatcaaa tgatgagcaa tgcctcggaa ctgcgtgggc tacatggcga tctgcatcat
     1561 gaaaacatca tgttctccag tcgcggctgg ctggtgatag atcccgtcgg tctggtcggt
     1621 gaagtgggct ttggcgccgc caatatgttc tacgatccgg ctgacagaga cgacctttgt
     1681 ctcgatccta gacgcattgc acagatggcg gacgcattct ctcgtgcgct ggacgtcgat
     1741 ccgcgtcgcc tgctcgacca ggcgtacgct tatgggtgcc tttccgcagc ttggaacgcg
     1801 gatggagaag aggagcaacg cgatctagct atcgcggccg cgatcaagca ggtgcgacag
     1861 acgtcatact agatatcaag cgacttctcc tatcccctgg gaacacatca atcttaccgg
     1921 agaatatcgt tggccaaagc cttagcgtag gatttcgccc tctcccgcaa acgacccctt
     1981 tattgtaggt gggccagttg gtgattttga acttttgctt tgccacggaa cggtctgcgt
     2041 tgtcgggaag atgcgtgatc tgatccttca actcagcaaa agttcgattt attcaacaaa
     2101 tccacgttgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat atatcatcat
     2161 gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg agccatat
//
